LOCUS       DASSNN010000001         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1512882, whole genome shotgun sequence.
ACCESSION   DASSNN010000001 DASSNN010000000
VERSION     DASSNN010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512882"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2173
                     /locus_tag="VFL84_00005"
     CDS             <1..2173
                     /locus_tag="VFL84_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7607028.1"
                     /translation="EGFFSVFTEPLHMGRVFSPEDGFNNTILSYQTWRDVFGSDPNIL
                     GATIRVNNAGLRVLGVAAEGFEFPLGTAIWTKIYQANGSENLFNLDGYARVKPGVSAA
                     QFQAELDVFAGRLESATAAVVARGGAVGWEAGRKLQFVGRPLLHDVVGDFRPTLFVVS
                     GATAILLLIACLNVANLLFSRGIARTSEIAVREALGAGRWRVFRQLMTESLVLCALGG
                     VLGSILAVVAVRVLQAIGPEDLPRFDAVSVSQDVFAFAAACVLLTVVIVGVAPALRSS
                     RGDLSSLMNEGGRSSSGGRARNRLFGTLVAAEIALAVVLVIGAGLLVRSYSAAHSADP
                     GFDPSRTLTFVLNVPGRLDLRAPRLDANRQVIGYEGTGYLPVARFYEELTRRIEALPG
                     VAAAGAVSAAPLQAGQFPIYPDSFGIVGDPESNVAGGGRVGYASQVAPEFFDALELRP
                     LAGRLLTADDRRDGAGVVVVNETFARAYLGANPIGRRITLPNPDIWRPLGLAFTLGER
                     LVNEAEVVGVIPDVKQRSITSPVQPAVYVPHEQWTMRRMSVVVRAEIDDPGTLIPAIR
                     NEIAQMDPTIPAVFALYSDVMTASVARQKLGAVSLVVFGLVSLTLAAVGIFGLVSYSA
                     RQRYNEIAVRSAMGADRGRVLKMFLGRALRLAGLGIGVGIAGAIALGQVIASQLYGVS
                     ALDPAVFLLVPLTMLGVTLLASYWPARRASRIDLSVALREN"
     gene            2224..2916
                     /locus_tag="VFL84_00010"
     CDS             2224..2916
                     /locus_tag="VFL84_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607029.1"
                     /translation="MRSQATHRWTQLGAAVAGALALGHAAAQEGALRYEITPYAAYRV
                     GGEFEPQQAEPKGTARELELEEGNAAGLILDIRTGAGNELAGGNGQWEVLYAHQSTEL
                     DTEPSFVGGPTLGLDVTHFQLGGAYLFNGDSATLVPFIAMTAGLARLEPRLDDADAED
                     FFSWSLGGGVHLRADKRIGVRLEARVFGTLIDDDGALFCVVSPTVNSCAVSVEGDQLY
                     QLETRVGIVGRF"
BASE COUNT          428 a         1062 c         1042 g          545 t
ORIGIN      
        1 ggagggcttc ttctccgtgt tcacggagcc gctccacatg gggcgggtgt tctcgcccga
       61 ggacggcttc aacaatacga tcctgagcta tcagacctgg agagacgtgt tcggctcgga
      121 cccgaacatc ctcggcgcga cgatccgcgt caacaacgcg ggattgcgcg tgctcggcgt
      181 cgccgccgag gggttcgagt ttccgctcgg cacggcaatc tggacgaaga tctatcaagc
      241 gaacggcagc gagaatcttt tcaacctcga cggttacgcg cgcgtcaagc cgggcgtctc
      301 ggccgcgcaa ttccaggccg agctcgacgt cttcgccggt cggctcgaat cggccacggc
      361 cgccgtcgtc gcgcgcggcg gcgccgtcgg ctgggaggcg ggccgcaagc ttcagttcgt
      421 cggccggccg ctgctgcacg acgtcgtcgg cgacttcagg cccacgctgt tcgtcgtctc
      481 gggcgcgact gcgattctgc tcctgatcgc gtgcttgaac gtcgccaacc tgctcttcag
      541 ccgcggcatc gcgcgcacga gcgaaattgc ggtccgagaa gcgctcggcg cgggacggtg
      601 gcgcgtgttt cggcagctca tgaccgagag cttggtgctc tgcgcgctcg gcggggtctt
      661 gggctcgatc ctcgccgtcg tcgcagtgcg cgtcctgcag gcgatcgggc ccgaagacct
      721 gccgcgcttc gatgccgtgt ccgtttcgca agacgtgttc gcgtttgccg ccgcgtgcgt
      781 gctgctgacc gtcgtcatcg tcggcgtcgc accggcgctg cgctcctcgc gcggcgatct
      841 cagcagcctc atgaacgaag gcgggcgctc ctcgtcggga gggcgcgcgc gcaaccgctt
      901 gttcggcacg ctcgtggccg ccgagatcgc gttggccgtc gtgctcgtca tcggcgccgg
      961 gctgctcgtg cgcagctact ccgccgctca ctccgccgac cctggcttcg atccgagccg
     1021 cacgttgacg ttcgtgctga acgttcccgg ccgcctcgac ttgcgcgcgc cgcgcctcga
     1081 cgcgaatcgc caagtcatcg gctacgaagg cacgggatac ctgccggtcg cgcgcttcta
     1141 cgaggagctc acgcggcgca tcgaggcgct gcccggcgtc gcggcggcgg gcgccgtatc
     1201 cgcggcgccg ctgcaagccg gtcagttccc gatttacccc gactcgttcg gcatcgtcgg
     1261 cgaccccgaa agcaacgtcg ccggcggcgg gcgcgtcggg tacgccagcc aggtcgcgcc
     1321 ggaattcttc gacgcgctcg agctccggcc gctcgcgggc cggctgctga ccgcggacga
     1381 ccggcgcgac ggcgccggtg tcgtcgtcgt gaacgagacg ttcgcgcgcg cgtacttggg
     1441 cgcgaatccc atcggccggc gcatcacgtt gcccaacccc gacatttggc ggccgctcgg
     1501 gctcgcgttc acgctcggcg agcggctcgt caacgaggcc gaggtcgtcg gcgtgatccc
     1561 cgacgtcaag cagcgcagca tcacctcccc ggtccagccg gcggtctacg tgccgcacga
     1621 gcagtggacg atgcgcagaa tgtcggtcgt cgtgcgtgcc gagatcgacg atcccggcac
     1681 gctgatcccg gccatccgca acgagatcgc gcagatggac ccgacgatcc ccgccgtctt
     1741 tgctctctac tcggacgtca tgacggcgtc ggtcgcgcgg cagaagctcg gagcggtgtc
     1801 gctcgtcgtg ttcgggctcg tctcgctgac gttggcggcc gtcggcatct tcggcctcgt
     1861 ctcgtattcg gctcgccagc ggtacaacga gatcgccgtg cggtcggcga tgggcgccga
     1921 ccgaggccgc gtcctcaaga tgttcctcgg gcgcgcgttg cggctcgcgg gcctcggcat
     1981 cggcgtcggc atcgccggcg cgatcgcgct cgggcaagtc atcgcgagcc agctttacgg
     2041 cgtcagcgcg ctcgacccgg ccgtgttctt gctcgtcccg ttgacgatgc tgggcgtgac
     2101 gctgctcgct tcctactggc ccgcgcgcag agcgtcgcga atcgatcttt cggtcgcgct
     2161 gcgagagaac tgatcccatt gtcgttccgc gcatccacgc tcgtcttgaa gtatttttgc
     2221 tctgtgcgat cgcaagcgac tcatcgatgg acgcagctcg gcgcggcagt ggccggcgcg
     2281 ctcgcgctcg ggcatgccgc ggcgcaagag ggcgcgctac gctacgagat cacgccgtac
     2341 gccgcgtacc gagtaggcgg cgaattcgag ccgcagcagg cggagccgaa aggcacggcc
     2401 cgcgagctcg agctcgagga aggcaacgcc gcaggactga tcctcgacat tcgcacgggc
     2461 gcgggcaacg agctcgccgg gggcaatggg cagtgggaag tgctctacgc gcatcagagc
     2521 accgagctcg acacggagcc ttcgttcgtc ggcggaccga cgctcggcct cgacgtgact
     2581 cattttcagc tcgggggcgc ctaccttttc aacggcgaca gcgcgacgct cgtgccattc
     2641 atcgcgatga ccgcgggtct ggctcgtctc gagccgcggc tcgacgacgc cgacgccgaa
     2701 gattttttct cttggtcgct cggcggcggc gtgcacttgc gcgcggacaa gcgcatcggc
     2761 gtgcggctcg aggcacgcgt gttcggcacg ctgatcgacg acgacggcgc gctgttctgc
     2821 gtggtctctc cgaccgtcaa ttcctgcgcc gtctcagtcg aaggcgacca gctctaccag
     2881 ctcgagacgc gcgtaggcat cgtcggccgc ttctagcgtt cccggtgccc gcgcggcgag
     2941 tatagttgcg tgcgcgcccg gctactcatc aacactcggg gagaaaagca atgacgaaaa
     3001 ccaccggaat cttggcgctc gcagtcttct gcgtcgtcgg cggtctcgct cgtgccgcag
     3061 acgtcagcac acgtgga
//
LOCUS       DASSNN010000002         5640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_504325, whole genome shotgun sequence.
ACCESSION   DASSNN010000002 DASSNN010000000
VERSION     DASSNN010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504325"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..830)
                     /locus_tag="VFL84_00015"
     CDS             complement(162..830)
                     /locus_tag="VFL84_00015"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7607030.1"
                     /translation="MKLRRSSLIAALAISCSAIVSAQQLPADLNATSRARLPYVQRKD
                     TDEASKRLFDIFVRNSNSPTDNLTGPLAFAAYNPYVATALLDLHDGAVGKGTLDAHVR
                     ELAILVACRATNYNFEWNGHEPAAVRAGVDQKVIDAVRTNGALTGLPDADAAVIRFGR
                     ELLNDRKMSSATFAKAKELYGPKGAMDLVAVMSTYAVSGFYAIAVDEHPAPGQKALPA
                     VAAH"
     gene            complement(851..1912)
                     /locus_tag="VFL84_00020"
     CDS             complement(851..1912)
                     /locus_tag="VFL84_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7607031.1"
                     /translation="MRARGLAAVVAIVAGLSASAQSLPIENIQLRGDRFRGLHYREMS
                     EEQRALFYNVVNSARAVTNTTGNGPFNTLLRSPQVGEQSQQLGNAIRFSSGLSGRERE
                     LATLMAGRAWTSTYEWYAHARYALDEGIDASVVTAIAEHRTPDLAAMPAEDAMVWRVS
                     DELLYTRRITDETYKQALAVLGERRLLSTVTIAAYYEYVSMLLNVDRYPLPDDAPPAA
                     AAAAHALEALSAAALYREPHGSVTLRTAIPNEDTERAIAALGASVEREEELSADARDF
                     ARRAVLRQWNRTRVHGTDNIAHKFATELLDTRFVSDTTFAAARDALGERGLVNLMVFM
                     GYSNILCAQQALAGSACEL"
     gene            complement(1915..3207)
                     /locus_tag="VFL84_00025"
     CDS             complement(1915..3207)
                     /locus_tag="VFL84_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607032.1"
                     /translation="MRRRHRVLAWIVAPMLAVSFECAAQMANLSERYVKLVLAVGVHD
                     ADYVDAYYGPPEWRTAAAEERRSIADLDADAVGWLRELADEVLAPGAGELERLRHSYL
                     ATQVESLRARLKMLRGERLTFDEESRALYDAVAPVHTEEEFQAVLRDLDARLPGRGPL
                     LDRYQAFRARFEIPKDRLAPVFAAAIEGCRARTLEHIALPPGESFTIEYVTNKSWSGY
                     NWYQGNYRSLIQINTDLPIYIERAIDLACHEGYPGHHVYNVLLEQHLVRERGWQEFTV
                     YPLFSPQSLIAEGTANYGIEVAFPTAERRRFERDVLFPAAGLDASRVDEYYDVLELVD
                     KLSYAGNEAARRYRDGKIDAAAAAKWLETYALYSPERAAQRVRFIDQYGAYVINYNLG
                     KDLVGSYVERHAGTDPERRWSEFAKLISSPRLPSGLKD"
     gene            complement(3235..3735)
                     /locus_tag="VFL84_00030"
     CDS             complement(3235..3735)
                     /locus_tag="VFL84_00030"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7607033.1"
                     /translation="MNSLRSCCVALAAAALAGTAFAEPEYTTIKLEIDIAKPAKEVWA
                     KVGGYCDISKWLSNVDCKITSGDGGIGTVRVLAGGRVTEILVAQTELSYGYTQPVREG
                     GFYNLYHGFMEARPVTATTSKMLYTLMYDVSNLADQAAKDADVARRKGMFETALKNMK
                     ALAEGQ"
     gene            complement(3816..4472)
                     /locus_tag="VFL84_00035"
     CDS             complement(3816..4472)
                     /locus_tag="VFL84_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HET7607034.1"
                     /translation="MRIDVVSDVACPWCAIGMSALDRALTRIGAELGEIEVHLQPFEL
                     NPAMGPEGADAAQYLSEKYGLSPEQLSANRARIVERGAAEGFRFGTRSHVWNTFDAHR
                     LLFWAGAEGPPGAQRGLKRALLTAYHGEDRNIGSADVLVELAASAGLPAGRAREVLER
                     GEFAEEVRQAERFWQEVGIHSVPAVIVDRKHLISGGQPSAVFEQALRQIAAQAASAGP
                     "
     gene            complement(4543..>5640)
                     /locus_tag="VFL84_00040"
     CDS             complement(4543..>5640)
                     /locus_tag="VFL84_00040"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET7607035.1"
                     /translation="TTPKPREVIAAVARYYETLGANVHRGLYPLAEAATEEYERARHR
                     VAALIGAQPAEIVFTRNATDSFNLVARALRLSADDEVVFPASEHHSNYLPWRVMAKPV
                     LVDIDEEAVPKWSQLKQKLGPRTRLVSVAHVSNVTGAVAPIEEWIETAHAAGIPVLVD
                     ASQSVAHLPLNVHALDVDFLGFSSHKMFGPNGVGVLYVRRDRFGDLGLGNVGGGMVAM
                     LADDRFEPLEAPFRYEAGTPNIEGVIGLGAAVDYLLRIGMANVAAHSRALAAQLMEGL
                     AGLPEATVLGRSVRDRIALATVSMPWPAMRQQDIARLLADAHSIFVSGGFHCAHVLHH
                     RLKLDGTLRASAHLYNDEGDIEAFLKALHDL"
BASE COUNT          908 a         1885 c         1871 g          976 t
ORIGIN      
        1 tgtattgctt gccgtcgacc tcgtaactga cgggtccgtt gacgatctgg ccgccgaggc
       61 tcgctctcca gagcatctcg cccgtgcgcg cgtcgagact cccggcgtgg tcgcttttag
      121 tccaagactc ttttcctgcg cctgtccgtc cgcggcggcg gtcagtgtgc cgcgactgct
      181 ggcagcgcct tctggccggg cgcggggtgc tcgtcgaccg caatggcgta gaagccgctg
      241 acggcgtagg tgctcatgac ggcgacgagg tccatcgcgc ctttcgggcc gtagagctcc
      301 ttcgctttcg cgaaagtcgc cgagctcatc ttgcggtcgt tcagcagctc gcggccgaag
      361 cggatcactg cggcgtcggc gtcggggagg cccgtcagcg cgccgttcgt gcgtacggcg
      421 tcgatgactt tctgatcgac accggcgcgc acggcggccg gctcgtgtcc gttccattcg
      481 aagttgtagt tcgtggcgcg gcacgcgacg aggatcgcga gctcgcgaac gtgcgcgtcg
      541 agcgtgccct tgccgactgc gccgtcgtga aggtcgagca gcgccgtcgc gacgtacgga
      601 ttgtaagcgg cgaacgcgag tgggcccgtc aagttgtccg tcgggctgtt gctgttgcga
      661 acgaagatgt cgaacaggcg cttcgacgcc tcgtcggtgt cctttcgttg gacgtacggt
      721 agccgcgcgc gcgacgtcgc gttgagatcg gccggcagtt gttgagcgct cacgatggcg
      781 ctgcatgaaa tggcgagagc ggcaatcagt gacgaacggc gaagcttcat cgggaatctc
      841 ctagttgtga ttagagctcg cacgcagagc cggccaaggc ctgctgcgcg cagaggatat
      901 tagagtagcc catgaacacc atgagattga cgaggccgcg ctcgcccaaa gcatcacggg
      961 ccgccgcgaa ggtcgtgtcc gagacgaagc gcgtatcgag cagctcggtc gcgaacttgt
     1021 gcgcgatgtt gtcggtgccg tgcacgcgcg tgcgattcca ttggcgcaac accgcgcgcc
     1081 gcgcgaaatc acgcgcatcc gcggacagct cctcctcacg ctcgacgctg gcgccgagtg
     1141 ccgcgattgc gcgctcggtg tcctcattcg gaatcgccgt ccgcagcgtc accgagccgt
     1201 gcggctcgcg gtacagcgca gcggcgctca gcgcctcgag cgcatgcgcg gcagccgcag
     1261 ccgccggcgg cgcatcgtcg ggcaacggat agcggtcgac gttcagcagc atcgacacgt
     1321 actcgtagta cgccgcgatc gtgaccgtgc tcagcagccg ccgctcgccg agcaccgcga
     1381 gcgcttgctt gtacgtctca tcggtgatgc gccgcgtgta gagcagctcg tcagaaacgc
     1441 gccagaccat cgcgtcctcg gccggcattg ccgcgaggtc gggcgtgcgg tgctcggcaa
     1501 tagcggtgac gaccgacgcg tcgatgcctt cgtcgagcgc gtaccgcgcg tgcgcgtacc
     1561 attcgtacgt cgacgtccac gcgcgcccgg ccatcagcgt cgcgagctcg cgctcgcggc
     1621 cggacaagcc ggacgagaag cggatcgcgt tgccgagctg ctgcgactgc tcgcctacct
     1681 gcggcgagcg cagcagtgtg ttgaacggcc cgttgccggt cgtgttcgtg acggcgcgcg
     1741 cgctgttgac gacgttgtag aacagcgcac gctgctcctc gctcatctct cgataatgca
     1801 agccgcgaaa gcgatcgccg cgcagctgga tgttttcgat cggcagcgac tgcgctgaag
     1861 ccgataggcc tgccacgatc gcgacgactg cggcgaggcc acgcgcgcgc actgtcagtc
     1921 cttgagcccg gacggcaagc gcggcgacga gatcagcttc gcgaactcgc tccaccgccg
     1981 ttccggatct gttcccgcat gccgctcgac gtagctcccg actaagtcct tgccgaggtt
     2041 gtagttgatg acgtacgcgc cgtactgatc gatgaagcgc acgcgctgcg cagcgcgctc
     2101 cggcgaatag agcgcgtagg tttcgagcca tttcgccgcc gcagcagcgt cgatcttgcc
     2161 gtcgcgatag cggcgtgccg cttcgttgcc ggcgtacgag agcttgtcga cgagctccaa
     2221 gacgtcgtaa tactcgtcga cgcgggacgc atcgagaccg gcggccggga acagcacgtc
     2281 gcgctcgaag cggcgccgct cggcggtcgg aaacgcgacc tcgatgccgt agttcgccgt
     2341 gccctctgcg atcaacgatt gcggcgagaa caatgggtac accgtgaact cctgccagcc
     2401 gcgctcgcgc acgaggtgct gctcgagcag cacgttgtag acgtgatggc cggggtagcc
     2461 ttcgtggcac gcgagatcga tcgcgcgctc gatgtaaatc ggcagatcgg tgttgatctg
     2521 gatcaggctt cgatagttgc cctgatacca gttgtagccg ctccagctct tgttcgtgac
     2581 gtactcgatc gtgaagctct cgccgggcgg cagcgcgatg tgctcgagcg tgcgagcgcg
     2641 gcatccttcg atcgccgccg cgaacacggg ggccaagcgg tccttcggaa tctcgaagcg
     2701 ggctcggaat gcctgataac ggtcgagcaa cggcccgcgt cccggcagcc gcgcgtcgag
     2761 atcgcgcaac accgcttgaa actcttcctc cgtgtgcacg ggcgcgacgg cgtcgtagag
     2821 cgcgcgggac tcctcgtcga acgtcaagcg ttcgccgcgc aacatcttca ggcgcgcgcg
     2881 caacgactcg acctgcgtcg cgaggtaact atgccgcaga cgctcgagct cgccggcgcc
     2941 gggcgccagc acttcatcgg cgagctcgcg cagccacccc accgcgtcgg catcgaggtc
     3001 ggcgatgctg cggcgctctt ccgccgcagc ggtgcgccac tcgggcggcc cgtaataggc
     3061 gtcgacgtag tccgcgtcgt gcacgccgac ggcgagcacg agcttcacgt agcgctccga
     3121 aaggttagcc atctgcgcgg cgcattcgaa cgacacggca agcatcgggg cgacgatcca
     3181 tgccagcacg cgatgccgtc gcctcatcgt tcatgctcct cgaaagacga agcgctattg
     3241 gccctcggcg agcgctttca tgttcttgag cgccgtctcg aacatgccct tgcggcgcgc
     3301 gacgtcggca tccttcgccg cttgatccgc gagattcgat acgtcgtaca tgagcgtgta
     3361 cagcatcttc gacgtcgtcg ccgtcaccgg gcgcgcttcc atgaacccgt gatacagatt
     3421 gtagaatccc ccttcccgca ccggctgcgt gtagccgtac gacagctctg tctgtgcgac
     3481 gaggatctcc gtgacgcgcc cgcccgcgag cacgcgcacc gtgccgatgc cgccgtcgcc
     3541 ggacgtgatc ttgcagtcga cgttgctgag ccacttcgag atgtcacagt agccgccgac
     3601 cttggcccag acttctttcg caggcttggc gatgtcgatc tcgagcttga tcgtcgtgta
     3661 ctcgggctcg gcgaaggccg tgccggccag ggcggccgcg gccagcgcga cgcaacagga
     3721 acgaaggcta ttcatttgtt ctccttattt agcgcgaatc gggtcggcgc cggctgatgg
     3781 tgttgctaaa cgaccgccat tgcaacgcct tgttttcaag gcccggccga cgcggcttgt
     3841 gccgcgatct gccgcagcgc ctgctcgaac acggcgctcg gctgcccgcc cgagatcaga
     3901 tgcttgcgat cgacgatgac ggccggcacc gaatggatgc cgacctcctg ccagaaccgc
     3961 tccgcttgcc gcacctcttc ggcgaactcg ccgcgctcga gcacttcgcg cgcgcgcccc
     4021 gccggcaacc cggccgaggc agcgagctcg acgagcacgt cggcggagcc gatgttgcgg
     4081 tcttcgccgt gataggccgt gagcagcgcg cgcttcaacc cacgctgcgc gccgggcggg
     4141 ccttccgctc cggcccagaa caacaggcga tgcgcatcga acgtgttcca gacgtgcgag
     4201 cgcgtgccga accgaaaccc ctcggccgcg ccgcgctcga cgatgcgtgc tcggttcgcg
     4261 ctcagctgct cggggctcag gccgtacttc tcgctgagat actgcgcggc gtcggcgcct
     4321 tcgggtccca tcgcgggatt cagctcgaac ggctgcaggt gcacctcgat ctcgcccagc
     4381 tcggcgccga tgcgcgtcaa ggcgcgatcc agagcgctca tgccgatcgc gcaccacggg
     4441 cacgcgacgt cggatacgac atcgattctc attgggcgat tctcatgggt tgcacctcgc
     4501 tcgcggcgac gacacttcag cgaattgtgc cacgcgcgcc gctcagaggt cgtgcagggc
     4561 cttgaggaag gcctcgatgt cgccttcgtc gttatagaga tgcgccgacg cgcgcaacgt
     4621 cccgtcgagc ttgagccgat ggtgcagcac gtgcgcgcaa tggaagccgc ccgagacgaa
     4681 gatcgagtgc gcgtcggcaa ggaggcgcgc gatatcctgc tggcgcatcg cgggccaggg
     4741 catcgatacc gttgcgagcg cgatgcggtc gcgcaccgag cggccgagca cggtcgcctc
     4801 cggcaagccc gcgagccctt ccatgagctg cgccgcgagc gcgcgtgaat gcgcggcgac
     4861 gttcgccatg ccgattcgca gcaagtagtc gacggcggcg ccgaggccga tgacgccttc
     4921 gatattcggc gtgccggcct cgtagcgaaa cggcgcctcg agcggctcga agcggtcgtc
     4981 ggccagcatc gcgaccatgc cgccgccgac gttgcccaat cccaaatctc cgaaccggtc
     5041 gcggcgcacg tacagcacgc cgacgccgtt cggcccgaac atcttgtggc tcgagaaccc
     5101 gaggaagtcc acgtcgagcg catgcacgtt cagcggcaga tgtgcgacgg actgcgaagc
     5161 gtcgacgagc acgggaatac cggccgcgtg cgcagtctcg atccactctt cgatcggcgc
     5221 aacggcgccc gtgacgttcg agacgtgcgc aacgctgacg agccgcgtgc gcggcccgag
     5281 cttctgcttc agctgcgacc acttcggaac agcctcctcg tcgatgtcga cgagaacggg
     5341 cttggccatg acgcgccacg gcaagtagtt cgaatgatgc tcggacgccg ggaacacgac
     5401 ttcgtcgtcg gcggaaaggc gcagcgcgcg agcgacgaga ttgaacgaat cggtcgcgtt
     5461 gcgcgtgaac acgatttccg cgggctgcgc gccgatgagc gccgcgacgc ggtggcgcgc
     5521 gcgctcgtac tcttccgtcg cggcctcggc gagcggatag aggccgcgat ggacgttcgc
     5581 gccgagcgtt tcgtaatagc gcgcgacggc cgcgatgacc tcgcgcggct tcggcgtcgt
//
LOCUS       DASSNN010000003         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_756581, whole genome shotgun sequence.
ACCESSION   DASSNN010000003 DASSNN010000000
VERSION     DASSNN010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756581"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFL84_00045"
     CDS             <1..315
                     /locus_tag="VFL84_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET7607036.1"
                     /translation="TKAGLNPRQMCDIINAGSGRNAATQDKFPRSILPGTFDFGFATG
                     LSYKDVRLCLQEADAQGVPMPVGAEVFQVLGMTKARYGADSDFTCIAKLYEEWAGVEM
                     RG"
     gene            329..1726
                     /locus_tag="VFL84_00050"
     CDS             329..1726
                     /locus_tag="VFL84_00050"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET7607037.1"
                     /translation="MATANRPASASAPLAVRMAHALAAYDYARLNPEVVAKAKLCVYD
                     LLSSALAAGEQPWSRQVIEVARRNSGGAAQGAGIIGTSDVVSLHDAALANGTLSHGLV
                     RDDMHVGSVSHLGTVLVPTLLALTQSAKASGKDFLTALVAGYEVGGKVGRMIMDVEVS
                     KIFRPTGTVGPIAGAAAGAKLLALTPEQTTAALALAANTAAGYNDWAGTGGSEMFFHN
                     GLAARSAVTAVQLAAEGAYVSPTALDGDAGILAAFRRPRPPTVPGLFADRPEILAVFF
                     KPVPACNFAQTPAQAALALAQRHAVSAADIDSVVVRVTKAAALYPGCDVSGPFEHILQ
                     AKMSIHYNVAAALAHGNFAERNYVPQQNLEVQALAKRIKVEIDPGFTSAFPAKQGAAV
                     VVRTRDGKTIEQSADNVEPTDADGVHERFFAAASATLGENRARELDALIGTLESCANT
                     AELARLTSKAKGTSQ"
     gene            1723..>2270
                     /locus_tag="VFL84_00055"
     CDS             1723..>2270
                     /locus_tag="VFL84_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7607038.1"
                     /translation="MSVTTHNGSAPRDSTHAASPRESVWRVALASMVGTSIEWYDFFI
                     FGAASALVFGRLFFPNVSELAGTLAAFATFAVGFVARPVGGLVFGHFGDRLGRKTMLV
                     VTLTMMGLATFAMGLMPTFATIGVWAPILMVVLRFVQGLAVGGEWGGAVLMATEHAGG
                     KRRGFFGSFAQIGSAVGGLMSTG"
BASE COUNT          343 a          789 c          789 g          349 t
ORIGIN      
        1 actaaggcgg gcttaaaccc gcggcagatg tgcgacatca tcaatgcggg cagcggccgc
       61 aacgcggcca cgcaggacaa gttccctcgc tcgatcctgc ccggcacctt cgacttcggc
      121 ttcgcgacgg ggctctcgta caaggacgtg cgcctgtgcc tgcaggaagc cgacgcgcag
      181 ggcgtgccga tgccggtcgg cgccgaggtg ttccaggtgc tcggcatgac gaaggcgagg
      241 tacggggcgg actcggactt cacgtgcatc gccaagctct acgaggagtg ggccggcgtc
      301 gagatgcgcg gctgaacgcg cgttcgacgt ggctaccgca aaccgccctg cttccgcctc
      361 cgcgccgctc gccgtgcgca tggcgcacgc gctcgccgcc tacgactatg cgcgcctgaa
      421 ccccgaagtc gtggcgaagg cgaagctctg cgtctacgac ctgctgtcgt cggcgctcgc
      481 ggccggcgag cagccgtgga gccgccaggt catcgaagtc gcgcggcgca actcgggcgg
      541 cgcggcgcag ggcgccggca tcatcggcac gtccgacgtc gtgtcgctgc acgacgccgc
      601 gctcgcgaac ggcacgttga gccacggtct cgtgcgcgac gacatgcacg tcggcagcgt
      661 gagccatctc ggcacggtgc tcgtgcccac gctgcttgcg ctgacgcaaa gcgcgaaagc
      721 gagcggcaag gatttcctga cggcgctcgt cgcgggctac gaggtcggcg gcaaagtcgg
      781 ccgcatgatc atggacgtcg aggtctcgaa gatcttccgg ccgacgggca cggtcggccc
      841 gatcgccggc gccgcggccg gcgcgaagct gctcgccctg acgccggagc agacgaccgc
      901 ggcgctcgcg ctcgcggcga ataccgcggc cggctacaac gactgggccg gcacgggcgg
      961 cagcgagatg tttttccaca atggcctcgc cgcgcgcagc gccgtgacgg ccgtgcagct
     1021 cgcggccgag ggcgcgtacg tgtcgccgac ggcgctcgac ggcgacgccg gcatcctcgc
     1081 ggcgttccgc cgccctcgcc cgccgaccgt gccggggctg ttcgcggacc ggcccgagat
     1141 actcgccgtg ttcttcaagc ccgtgccggc gtgcaacttc gcgcagacgc cggcgcaagc
     1201 cgcgctcgcg ctcgcgcagc ggcacgccgt aagcgccgcc gacatcgaca gcgtcgtcgt
     1261 gcgcgtgacg aaggccgcgg cgctctatcc gggctgcgac gtgagtggac cgttcgagca
     1321 cattttgcaa gcgaagatga gcatccacta caacgtcgcc gcggcgctcg cgcacggcaa
     1381 cttcgccgag cgcaattacg ttccgcagca gaacttggaa gtgcaagcgc tcgcgaagcg
     1441 catcaaagtc gagatcgatc ccggcttcac gagcgctttc ccggcgaagc aaggcgccgc
     1501 cgtcgtcgtc cgcacgcgcg acggcaagac gatcgagcaa agcgctgaca acgtcgagcc
     1561 cacggatgcc gacggcgtgc acgagcgctt cttcgcggcc gcatcggcca cgctcggcga
     1621 gaaccgcgcg cgtgagctcg acgcgctgat cggcacgctc gaatcctgcg cgaacaccgc
     1681 ggagctcgcg cgcctcacca gcaaagcaaa aggcacctcg caatgagtgt gacgacgcac
     1741 aacggttctg cgccgcggga ttccacgcat gctgcgtcgc cgcgcgaatc ggtgtggcgc
     1801 gtcgccttgg cgagcatggt cggtacctcc atcgagtggt acgacttctt catcttcggc
     1861 gccgcgtcgg cgctcgtgtt cggccggctg ttctttccca acgtctccga gctcgcgggc
     1921 acactcgccg cgttcgcaac gttcgccgtc ggcttcgtgg cacggcccgt cggcggtctc
     1981 gtgttcggtc acttcggcga ccgcctcggg cgcaagacga tgctggtcgt cacgttgacg
     2041 atgatgggcc tcgcgacgtt cgcgatgggc ttgatgccga cctttgccac gatcggcgtg
     2101 tgggcgccga tcctcatggt cgtgctgcgc ttcgtgcagg gcctcgccgt cggcggcgag
     2161 tggggtggcg cggtgctgat ggccaccgag cacgcgggcg gaaagcggcg cggcttcttc
     2221 ggcagcttcg cgcaaatcgg cagcgccgtc ggcggcttga tgtcgacggg
//
LOCUS       DASSNN010000004         4232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_252891, whole genome shotgun sequence.
ACCESSION   DASSNN010000004 DASSNN010000000
VERSION     DASSNN010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252891"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFL84_00060"
     CDS             <1..378
                     /locus_tag="VFL84_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607039.1"
                     /translation="LPERIAAIAPMAGLRWPGQCRGRAVPVLTFHGLADPQNTYDGHV
                     ASRAGEWDESVPEALAGWAKHNGCRGDAVLDDPPGPLSTLSYKGCDAEVRLVRVDGLV
                     HAWARNEVDATAEMWRFFSSQHL"
     gene            511..1125
                     /locus_tag="VFL84_00065"
     CDS             511..1125
                     /locus_tag="VFL84_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7607040.1"
                     /translation="MYKAITVAGVLLALTAGPALAQRNRDDDRAGRNLEGFYLGGGVG
                     DFSSEIRDIDRPGDVNDVGIDFTDSSNAMKVFAGWNFNRFFAVQGDYIDFGDSSGAVA
                     PSVGSTAQMGSNDVQGLAPSIVGTIPIGPIELFGRLGVIFYEIDANLTGGRLIDDSGS
                     DMVWSAGLGIDILDRANLRLEYEEIDIPQLDKADALWLNFAWKF"
     gene            complement(1248..2873)
                     /locus_tag="VFL84_00070"
     CDS             complement(1248..2873)
                     /locus_tag="VFL84_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET7607041.1"
                     /translation="MTTPTRAVALLLRRAALALLLLPALAGAQDAARMDAVISARVAD
                     KSFMGAVLVARGDQVILSKGYGFANLEWNVPNTPSTKFRLGSITKQFTAASILLLAER
                     GKLTVEDPIKKHWPDAPSAWEAVTIFHLLTHSSGIVNFTNQPDFMEWKMHPATPEQTL
                     AHIRDKPLEFTPGERMSYSNSGYVLLGMIVERVSGQSYADFVRDNIFKPLGMNDSGYD
                     VSAAILPQRAAGYTPGFSNAPYTDMSWPHGAGGLYSTTEDLLRWTQGLFGNKVLSAAS
                     VEKMTTPYKGDYAFGLSVSTVGGRKIIQHNGGIEGFNTHLAYYPDGKITVAVLANVNG
                     QAPGLLAGQLGQIANGNEVRLSSERKAIELPRDKLDRLAGSYELTPMATMRITVVGTQ
                     LQSQLGGQPVVPLFAESETVFFPRVVEAELTFELDAAGKATALVLKQNGQERRAPRIA
                     ERKEVVLPLAVLQRYPGTYRLQPGFDIVITLENGQLMSQATGQAKAPLYAESERKFFL
                     KVANAQIEFVGDGERATELVLNQGGRETHAPRQ"
     gene            complement(3055..3822)
                     /locus_tag="VFL84_00075"
     CDS             complement(3055..3822)
                     /locus_tag="VFL84_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7607042.1"
                     /translation="MPEPVSTAQYFPWNGGAVFVGTAGTFPAHAHQAIQICFLFEGRV
                     RLRPTDADEWRDYEVAIVPSRRSHGMDGASIHCGATIFVEPETREGRILTERYLQGGI
                     TELGREPLDDKVRDVRAAALERRGASAIVERARAIVQALTQHTEPSATSDERILRAVK
                     YINEHLTQPITLAQVARVAYLSPSRFRHLFAEQTGMRLREYILWRRFVSVWEQRMKGV
                     SLSEAAHAAGFADSAHLARTSRRMIGIPPSLMDVQRE"
     gene            complement(3849..>4232)
                     /locus_tag="VFL84_00080"
     CDS             complement(3849..>4232)
                     /locus_tag="VFL84_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HET7607043.1"
                     /translation="GLDVFALAPSEFLSANEIGAAALASQGGRFNPQQQFPVTWPAEP
                     VVARAYVDQLRRAPSASAATLDEISGALDLVAPQLTAGGKDVRLAERLTSLAQSVSRG
                     GGDALTIKRKKGLADTLTGLSARLR"
BASE COUNT          687 a         1408 c         1413 g          724 t
ORIGIN      
        1 ctccccgaga gaatcgccgc gatcgcgccg atggccggcc tgcgctggcc cgggcaatgc
       61 aggggccgcg ccgtccccgt gctcacgttc cacggcctcg ccgatccgca aaacacctac
      121 gacggccacg tcgcaagccg cgcgggcgaa tgggacgaga gtgtgccgga agcgctcgcg
      181 ggctgggcca agcacaacgg ctgccgcggc gacgccgtcc tcgacgatcc gccggggccg
      241 ctatcgacgc tgagctacaa aggctgcgac gccgaagtgc gcctcgtgcg cgtcgacggt
      301 ctcgtgcacg cgtgggcgcg caacgaggtc gacgcgacgg ccgagatgtg gcgcttcttc
      361 agcagccagc acctgtagcg cgtaagccac tttcgcgcgc gtagcgcgga agttccgcgc
      421 cattcccaaa agttctctgc gctagggagc gcctaaactg gcatggagat tgccttcttc
      481 tctggcttcc agaccaaaag gaggtgtgag atgtacaagg caatcactgt cgcgggagtg
      541 ctactggcgt tgacggcggg acccgcgctc gcccagcgaa accgagatga cgatcgtgcc
      601 ggccgtaacc tcgaggggtt ctatctcggc ggcggcgtcg gcgatttttc atccgagatc
      661 cgcgatatcg accgtcccgg cgacgtaaac gacgtaggga tcgacttcac cgacagcagc
      721 aacgccatga aggtgttcgc cggctggaac ttcaatcgct tttttgcggt gcaaggcgac
      781 tacatcgact tcggcgattc gagcggcgcc gtcgcgccct cggtcggctc gactgcgcag
      841 atgggttcga acgacgtgca aggcctcgcg ccgagcatcg tcggcacgat accgattggg
      901 ccgatcgagc tgttcggccg cctcggcgtg atcttctacg agatcgacgc gaacctgacc
      961 ggcggccgcc tcatcgacga tagcggctcg gatatggtgt ggagcgccgg tctcggcatc
     1021 gacatcttgg accgcgcgaa cctgcggctc gagtacgagg agatcgacat cccgcagctc
     1081 gacaaagccg acgcgctctg gctcaacttc gcgtggaagt tctaacgccc taggcccgaa
     1141 aaaggggaca gatctatttt ccgcgcgcag atggaccagc actcgagcga cacacgtgta
     1201 gcgcggaaaa tagatctgtc ccctttttcg gtcgtcccct ttttcggtca ttgccgcggg
     1261 gcgtgcgtct cgcggccgcc ctgattcagc acgagctcgg tagcgcgctc gccgtcgccg
     1321 acgaactcga tctgcgcgtt cgcgactttc aagaaaaatt tgcgctcgga ttcggcgtac
     1381 agcggcgctt tggcctggcc cgtcgcctgc gacatgagct gaccgttctc gagcgtgatg
     1441 acgatatcga agccgggttg tagccgatac gtgcccggat agcgctgcag cacggcgagc
     1501 ggcagcacga cctctttgcg ttccgcgatg cgcggcgcgc gccgctcctg cccgttttgc
     1561 ttcagcacga gcgccgtcgc cttgccggcc gcgtcgagct cgaacgtgag ctcggcctcg
     1621 acgacacgcg ggaagaacac cgtctcgctc tccgcgaaca gcggcacgac cggttgcccg
     1681 ccgagctggc tctgcagctg cgtgccgacg accgtgatgc gcatcgtcgc catcggcgtg
     1741 agctcgtacg agcccgcgag ccgatcgagc ttgtcgcgcg gcagctcgat cgcttttcgc
     1801 tccgacgaga ggcgcacctc gttgccgttg gcgatctggc cgagctggcc cgcgagcagc
     1861 ccgggcgcct ggccgttgac gttcgcgagc acggcgaccg tgatcttgcc gtcggggtag
     1921 tacgcgagat gcgtgttgaa gccttcgatg ccgccgttgt gctggatgat cttgcggccg
     1981 ccgaccgtgc tcacgctgag gccgaacgcg tagtcgccct tgtacggcgt cgtcatcttc
     2041 tcgacggaag cagcggagag caccttgttg ccgaacaggc cttgcgtcca acgaagcaaa
     2101 tcctcggtcg tcgagtacag cccgccggcg ccgtgcggcc agctcatatc ggtgtaagga
     2161 gcattgctga accccggcgt gtagccggcc gcgcgctgcg gcaggatcgc ggcgctgacg
     2221 tcatagccgc tgtcgttcat gccgagcggc ttgaagatgt tgtcgcgcac gaaatccgcg
     2281 taactctggc cgctcacgcg ctcgacgatc atgccgagca gcacgtaccc cgagttgctg
     2341 tagctcatgc gctcacccgg cgtgaactcg agcggcttgt cgcgaatgtg cgcgagcgtt
     2401 tgctccggcg tcgccggatg catcttccac tccatgaagt cgggttggtt cgtgaagttc
     2461 acgatgccgg agctgtgcgt gagcagatga aagatcgtga cggcctccca cgcggacggc
     2521 gcatccggcc agtgcttctt gatgggatct tcgacagtga gcttgccgcg ttccgcgagc
     2581 agcaagatcg aagccgccgt gaactgcttc gtgatcgagc ccaagcgaaa tttcgtgctc
     2641 ggcgtgttcg gcacattcca ttcgaggttc gcgaagccgt agcccttgct caagatgact
     2701 tggtcgccgc gcgcgacgag caccgcgccc atgaagctct tgtcggcgac gcgggcgctg
     2761 atcaccgcgt ccatgcgcgc ggcgtcttgc gcgccggcga gcgcgggcag cagcagaagc
     2821 gcgagcgctg ctcggcgcag taggagggcg acggcacggg tcggcgttgt cattgttgaa
     2881 ccccggggag tggtcggccg gaagccattc tcgacccgcg ccgtagggcg cacaagacgc
     2941 cgtcagcgga gatgtctcga agttgcgcgg cagccgggcg ctgccgcgct cctcgactgc
     3001 gccgttacgc cgccactgta ccggcggctc gcggaagcgc cttgaacgaa acggctattc
     3061 tcgttgcacg tccatcaacg acggcgggat gccgatcatg cgccggctcg tgcgcgcgag
     3121 atgcgccgag tccgcgaagc ccgcggcgtg cgcggcctcc gacagcgaga cgcccttcat
     3181 cctctgctcc caaacgctga cgaagcgccg ccacaggatg tactcgcgca agcgcattcc
     3241 cgtctgctcc gcgaacagat gacgaaagcg gctcggcgac agatacgcga cgcgcgcgac
     3301 ctgcgcgagc gtgatcggct gcgtgagatg ctcgttgatg tacttgaccg cgcgcaggat
     3361 gcgctcgtcg ctcgtcgcgg acggctcggt gtgctgcgtc agcgcctgca cgatggcgcg
     3421 ggcgcgctcg acgatcgcgc tcgcgccgcg ccgctcgagc gccgcggcgc gcacgtcgcg
     3481 tactttgtcg tcgagcggct cccgaccgag ctccgtgatg ccgccttgca gataccgctc
     3541 ggtgagaatg cgcccctcgc gcgtctcggg ctcgacgaag atcgtcgcgc cgcagtggat
     3601 cgacgcgccg tccatgccgt gcgaacgccg cgacggcacg atcgcgacct cgtagtcgcg
     3661 ccattcgtcg gcatcggtcg gacgcagccg gacgcggccc tcgaacagga agcagatttg
     3721 gatcgcctga tgcgcgtgcg cggggaacgt gccggccgtg ccgacgaaga cggctccgcc
     3781 gttccacggg aaatactgcg ccgtgctgac gggctcgggc atcgagtgca gcttcgcgag
     3841 cgcgcgcgtt accggagccg tgccgagagg cccgtcagcg tgtcggcgag gcccttcttg
     3901 cgcttgatcg tgagcgcgtc gccgccgccg cgtgagacgc tctgcgcgag cgaggtcaag
     3961 cgctcggcca aacgcacgtc tttcccgccc gcggtgagct gcggcgcaac gagatcgagc
     4021 gcgccggaaa tttcgtcgag cgtcgctgcc gatgccgacg gcgcgcgccg caactgatcg
     4081 acgtacgcgc gcgcgacgac cggctcggcg ggccacgtca ccggaaactg ctgctgcgga
     4141 ttgaagcggc cgccctgcga agcgagcgcg gcggcgccga tctcgttcgc cgacaggaat
     4201 tcgctcggcg ccaacgcgaa cacgtcgagc cc
//
LOCUS       DASSNN010000005         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_378985, whole genome shotgun sequence.
ACCESSION   DASSNN010000005 DASSNN010000000
VERSION     DASSNN010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378985"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFL84_00085"
     CDS             <1..387
                     /locus_tag="VFL84_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607044.1"
                     /translation="VRFKPVAGTIDRAAGLVWRYRDENNYYLVRASALEDNVALYKIE
                     GGRPVSLEPAGKGGERAVAYLVPAGRWSTLRVAFMAARFVVSLDSKPLFEVEDTTFTG
                     PGKVGVWTKADSVTYFDEFDLLVGAE"
     gene            complement(406..1308)
                     /locus_tag="VFL84_00090"
     CDS             complement(406..1308)
                     /locus_tag="VFL84_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7607045.1"
                     /translation="MKNLIAIGTLAALAVVCGGAHAQQRGQQPPPQGVGTPSADPGAG
                     FGPAQLTTFKVRDNFYMIRSGQSGNCSVLIADDGVVLIDNKFEMDHDGIIAKLREFTD
                     KPLLYVINTHMHGDHSGGNAKNQALGAKVIAHENARFQMAMTQTAGLPTITLEDHMRL
                     YHGEFILDIYWLGRGHTDGDVVVHLPKQNMVFTGDLFATWDPYVTLIDYPGGGSLREW
                     TRTLERIEALPFDTVIPGHGGPTDRAHLATYKAQLVRMQDMVREMNRAKKSREDIQKM
                     LETEFGWRGFVTTLGLDGVIREMQ"
     gene            complement(1308..2342)
                     /locus_tag="VFL84_00095"
     CDS             complement(1308..2342)
                     /locus_tag="VFL84_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607046.1"
                     /translation="MKSRVSSLCALGACALGACALAACTQQSDVSVAEQATPPSDWQH
                     PVTEWGEPDLRGMWPIGHLTGVPLQRPANFGERRLLTDEELAARSQQYTGRQTAYQQE
                     IDTNKMGMGHWVEWGTANRLTSLIVDPPNGQLPALTPEGERRRAAMRSGWMTIAFDHW
                     TDFDNWDRCITRGLPASMLPAYYNNGVEILQSPGYVVVRLEMIHEARVIPITSEPVDS
                     GAKAWFGYSRGHWEGNTLVVETTNFNGQGSSTNIHTIGSPPFNNTPISEEYVLTERFT
                     RTGPDTLTYQATLNDPVIWTAPWTVELPWQRDDSYEIFEYACHEDNSMIRNYIVTSRA
                     ERANQRGARR"
     gene            complement(2426..2722)
                     /locus_tag="VFL84_00100"
     CDS             complement(2426..2722)
                     /locus_tag="VFL84_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607047.1"
                     /translation="MSTLVFCVFDLSHATREDYLYAYMDLADAGLRQTIKSESGPSFR
                     LPASAVMGTFDGRSVEDIRSTVSKKVQGIFKSRGLKGTFFLVASGDWACAGEAT"
     gene            complement(2843..>3041)
                     /locus_tag="VFL84_00105"
     CDS             complement(2843..>3041)
                     /locus_tag="VFL84_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607048.1"
                     /translation="TAASRAEAERLGLGPFLEEAGIATGTIVVLDGRTKEILAWINGS
                     RDFDDYRTAIDAALGAPQRAL"
BASE COUNT          473 a          987 c         1015 g          566 t
ORIGIN      
        1 gtgcgcttca agcccgtcgc cggcaccatc gatcgcgcgg cgggtctcgt ctggcggtat
       61 cgcgacgaga acaactacta cctcgtgcgc gcgagcgctc tcgaagacaa cgtcgcgctc
      121 tacaagatcg agggcggccg gccggtctcg ctggagccgg cggggaaggg cggcgagcga
      181 gccgtcgcct acttggtgcc ggccgggcgg tggagcacgc tgcgcgtcgc gttcatggcc
      241 gcgcggttcg tcgtgtcgct cgacagcaag ccgctgttcg aggtcgagga cacgacgttc
      301 accgggcccg gcaaagtcgg cgtgtggacg aaggccgaca gcgtcacgta cttcgacgag
      361 ttcgacttgc tcgtcggcgc cgagtgagca gcggcggcgc cgggcctact gcatctcgcg
      421 aatgacgccg tcgaggccga gcgtcgtgac gaatccgcgc cagccgaact ccgtctcgag
      481 catcttctga atgtcttcgc gcgacttctt cgcgcggttc atctcccgca ccatgtcctg
      541 cattcgcacg agctgcgcct tgtacgttgc caggtgcgcg cgatccgtcg gtccgccatg
      601 gcccggaatc acggtgtcga acggcagcgc ctcgatgcgc tcgagcgtcc gcgtccactc
      661 gcgcaggctg ccgccgcccg ggtagtcgat cagcgtcacg tacgggtccc acgtcgcgaa
      721 cagatcgccc gtgaagacca tgttctgctt cggcaagtga acgacgacgt cgccgtcggt
      781 gtggccccgc ccgagccagt agatgtcgag gatgaactcg ccgtggtaga ggcgcatgtg
      841 atcttcgagc gtgatcgtcg gcaggcccgc cgtctgcgtc atggccatct ggaaccgcgc
      901 gttctcgtgc gcgatgacct tcgcgcccaa tgcctgattc ttggcgttgc cgcccgagtg
      961 atcgccgtgc atgtgcgtgt tgatgacgta cagcagcggc ttgtccgtga actcgcgcag
     1021 cttcgcgatg atgccgtcgt ggtccatttc gaacttgttg tcgatcagca cgacgccgtc
     1081 gtcggcgatc agcaccgagc agttgccgga ttggccgctg cggatcatgt agaaattgtc
     1141 gcggaccttg aacgtcgtga gctgtgccgg gccgaagccg gcgccgggat ccgcggaagg
     1201 ggtgccgacg ccttgcggcg gcggttgttg cccacgctgc tgcgcgtgcg cgccgccgca
     1261 aactacggcc agcgccgcga gcgtaccgat tgcgatcagg ttcttcatta gcgtcgggct
     1321 cccctctgat tggcgcgctc ggcgcgcgac gtcacgatgt aattgcggat catcgagttg
     1381 tcctcgtggc acgcgtactc gaagatctcg tagctgtcgt cgcgctgcca cggcagctcg
     1441 acggtccagg gcgcggtcca gatcacgggg tcgttcagcg tggcctgata cgtcagcgtg
     1501 tccggccccg tgcgcgtgaa acgctcggtc agcacgtact cctcgctgat cggagtgttg
     1561 ttgaacggcg gcgagccgat agtgtggatg ttcgtcgagc tgccttggcc gttgaagttc
     1621 gtagtctcga cgacgagcgt gttgccttcc cagtggccgc gcgagtagcc gaaccaggct
     1681 ttcgcgccgc tgtcgacggg ctcgctcgtg atcggaatca cgcgtgcctc gtgaatcatc
     1741 tcgagccgga cgacgacgta gcccggcgac tgcaggatct cgacgccgtt gttgtagtac
     1801 gccggcagca tcgatgcggg caagccgcgc gtgatgcacc ggtcccagtt gtcgaagtcg
     1861 gtccagtgat cgaacgcgat cgtcatccag ccgctgcgca tggccgcgcg gcggcgctcg
     1921 ccctcgggcg tcaacgcggg cagctggccg ttcggcggat cgacgatcag cgacgtcaaa
     1981 cgattcgccg tgccccattc gacccagtgg cccatgccca tcttgttggt gtcgatctct
     2041 tgctgatagg cggtctgcct gccggtgtac tgctgcgaac gcgcggcgag ctcctcgtcg
     2101 gtcaagagcc gccgctcgcc gaagttggcg ggccgctgca gcggcacacc ggtcaagtgc
     2161 ccgatcggcc acatgccgcg cagatccggc tcgccccatt cggtgaccgg gtgttgccag
     2221 tcggagggcg gcgttgcctg ctccgcgaca ctgacgtcgg attgctgcgt acatgccgcc
     2281 aacgcgcagg cgccaagcgc gcaggcgcca agcgcgcaca gcgaggagac tctgcttttc
     2341 atgcgattga cctcttcgag cgcgcgtggc tcggcgtcga acgcggattg tcgaacagtg
     2401 gcaatgccat ggcaacgaat ggccgttacg tcgcttctcc tgcgcaggcc cagtcgccgc
     2461 tcgcgacgag gaagaaagtg cctttcagcc cgcgcgactt gaagattccc tgcaccttct
     2521 tgctgacggt gctgcggatg tcctcgacgc tgcggccgtc gaacgtgccc atgaccgcgg
     2581 aagcgggcag cctgaagctc gggccgctct cggacttgat cgtttggcgc agccccgcgt
     2641 ccgcgagatc catgtatgcg tagagataat cttcacgagt cgcgtgcgac agatcgaaaa
     2701 cgcaaaaaac cagagtcgac atggctcacc catccaaaaa ttcgaccggt atagtacgcc
     2761 gcgcgcatga gcacggcgcg cgaatgcgag gacgtgcttc tgctcacggc gtccggcaca
     2821 ccgtcatcat gcccgtgacg gcttagagcg cgcgttgcgg cgcgccgagg gctgcgtcga
     2881 tcgcggtgcg atagtcgtcg aagtcgcggc tgccattgat ccacgcgagg atctccttgg
     2941 tgcggccgtc gagcacgacg atcgtgccgg tcgcgatgcc ggcttcctcg aggaacgggc
     3001 cgaggccgag ccgctcggcc tcggcgcgac tcgcggccgt c
//
LOCUS       DASSNN010000006         2064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1135425, whole genome shotgun sequence.
ACCESSION   DASSNN010000006 DASSNN010000000
VERSION     DASSNN010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135425"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VFL84_00110"
     CDS             complement(<1..841)
                     /locus_tag="VFL84_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET7607049.1"
                     /translation="MTTRALIGISGWRYAPWRGKFYPPGLPERDELKFAAERFPTIEL
                     NGSFYSLQRPEYYERWYEQVPDDFVFAIKGSRYITHMLRLRGVEKALANLFASGVLAL
                     EDKLGPMLWQLPPTLKLDVQRLEDFFALLPRTTKEAAKLARRHDERVKGRAYLRAHAD
                     RDLRHALEVRHESFATAELVELLRAHDIALVVADTAGRWPFLEDVTADFVYVRLHGDV
                     ELYKSGYTDAALERWAARIRAWRDGRTPPGAKLLAEPARRKHRDVYVYFDNDVKVHAP
                     YDAM"
     gene            910..>2064
                     /locus_tag="VFL84_00115"
     CDS             910..>2064
                     /locus_tag="VFL84_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET7607050.1"
                     /translation="MDESPVERALEPRWPRRVLLLGASGLIGSAVLARFGAQGVAVRA
                     VARRLEPGVQHGDVEWRALDLARLVEPADWLPHLDGVDAVVNCAGVLQGKGARAVHVD
                     SALALYAACESAGVRRVVHLSAIGVEREALSEYSTTKRAGEQALMATELDWVVLRPSV
                     VVGRAAYGGSALFRGLAALPVVLEFPDAGPLQVVQLDEVVATIVALSRHGAPARVALD
                     VAGPERLELPEIVDTFRSWLRFPPASHWRVPRWLAACGFALGDFARLLGWRPPIGAVA
                     RRELKRGAVGDPSAWRELMAAEPARLADALAREPASVQERWFARLYFLKAAVFVVLAL
                     FWIGTGMISLTVGWEEGLSYLLEGGVPERFAAPGVVAGALADLAIGVGIAV"
BASE COUNT          257 a          713 c          758 g          336 t
ORIGIN      
        1 tcatggcgtc gtacggtgcg tgcaccttga cgtcgttatc gaagtagacg tagacgtcgc
       61 gatgtttccg tcgcgccggc tccgcgagca gcttcgcccc cggcggcgtg cgcccgtcgc
      121 gccacgcgcg aatgcgcgcg gcccagcgct cgagcgccgc gtccgtgtag ccgctcttgt
      181 agagctcgac gtcgccgtgc aagcggacgt agacgaagtc ggccgtcacg tcttcgagaa
      241 acggccagcg gcccgccgtg tcggcgacga cgagcgcgat gtcgtgtgcg cgcagcagct
      301 cgacgagctc cgccgtcgcg aagctttcgt gcctgacctc gagcgcatgg cgtaggtcgc
      361 gatcggcgtg cgcgcgcaga tacgcgcggc ctttgacgcg ctcgtcgtga cggcgcgcga
      421 gcttcgcggc ctccttcgtc gtgcgcggca gcagcgcgaa gaagtcctcg agccgctgca
      481 cgtcgagctt cagcgtaggc ggcagctgcc agagcatcgg cccgagcttg tcctcgagcg
      541 cgagcacgcc ggacgcgaac agattcgcga gcgccttctc gacgccgcgc aatcgaagca
      601 tgtgcgtgat gtagcgcgag cccttgatcg cgaacacgaa gtcgtcgggc acctgctcgt
      661 accagcgctc gtagtactcg ggccgctgca gcgagtagaa gctgccgttg agctcgatcg
      721 tcggaaaccg ctcggccgcg aacttcagct cgtcgcgttc gggcagtccc ggtggataga
      781 acttgccgcg ccacggcgcg tagcgccaac ccgagatgcc gatcaatgcc cgcgtcgtca
      841 tgccgcagcg acgatgcaaa ccatatgcca gcgcgggcgc cgtggcatgc tccttgctat
      901 cttgcggcga tggacgaatc ccccgtcgaa cgcgcgctcg agccgagatg gccgaggcgc
      961 gtgctgctgc tcggcgcgag cggattgatc ggcagcgccg tgctcgctcg cttcggcgcg
     1021 caaggcgttg cggtgcgagc cgtcgcgcgg cggctcgagc cgggcgtcca gcacggcgac
     1081 gtcgagtggc gcgcgctcga tctcgcgagg ctcgtcgagc ccgccgactg gctgccgcat
     1141 ctcgacggcg tcgacgccgt cgtgaactgc gccggcgtgc tgcaaggcaa gggcgcgcgc
     1201 gccgtgcacg tggacagcgc gcttgcgttg tacgcggctt gcgagagcgc cggcgtgcgc
     1261 cgcgtcgtgc atctctccgc gatcggagtc gagcgcgagg ctttgagcga gtactcgacg
     1321 acgaagcgcg ccggcgagca agcgctgatg gcgacggagc tcgactgggt cgtcttgcgg
     1381 ccctccgtcg tcgtcggccg cgccgcctac ggcggcagcg cgctgttccg cggcctcgcg
     1441 gcgctgccgg tcgtgctcga gtttcccgac gcgggtccgc ttcaggtcgt gcagctcgac
     1501 gaggtcgtcg cgacgatcgt cgcgttgtcg cggcacggag cgccggcgcg cgtcgcgctc
     1561 gacgtcgcgg ggcccgagcg gctcgagctg ccggaaatcg tcgacacgtt ccgcagctgg
     1621 ctgcgctttc cgccggcgtc gcactggcgc gtgccgcgct ggctcgccgc gtgcggcttc
     1681 gcgctcggcg acttcgcgcg gctgctcggc tggcggccgc ccatcggcgc cgtggcgcgc
     1741 cgcgagctca agcgcggcgc ggtcggcgac ccgagcgcgt ggcgcgagct catggccgcc
     1801 gagcctgcgc ggctcgccga cgcgctcgcg cgcgagcccg cgtcggtcca ggagcgctgg
     1861 ttcgcgcggc tgtatttcct gaaggcggcg gtgttcgtcg tgctcgcgtt gttctggatc
     1921 ggcacgggca tgatctcgct gacggtcggg tgggaggaag gcctgtcgta tctcctcgaa
     1981 ggcggagtgc ccgagcgatt cgccgccccc ggcgtcgtcg cgggcgcgct cgccgatctc
     2041 gcgatcgggg tcggcatcgc ggtg
//
LOCUS       DASSNN010000007         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1514538, whole genome shotgun sequence.
ACCESSION   DASSNN010000007 DASSNN010000000
VERSION     DASSNN010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514538"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VFL84_00120"
     CDS             <1..1023
                     /locus_tag="VFL84_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7607051.1"
                     /translation="PHAVLFEMLFGAWTAKILAEVVRLDVPDALARGPLTADELVRKA
                     GIRAHPGALRRALRACAALGIVTEDSAGRFGPTRLSALLTRDTPGTLKRFVEYSGGTL
                     WKVWTGLPEALATGHSQARAQLGLDFFDYLGANPQALEEFGETLKAHSAVANIGVLER
                     YDFGGIRTLVDVGAGYGPLTIAVLEKYAEIRGVVFDLPEVIEAAPSSLPVKSDAVARR
                     LTYTAGDMFEEVPPADAYVLKLIMHDWDDASCAKILANVCARLARGGRVICIDNVMPP
                     LGDVSDVPQKLLDVNMMLLLPGKERTRVEWEALYRGAGLSISAMIPVPDAFNMFVIEG
                     RRAGEA"
     gene            1082..1657
                     /locus_tag="VFL84_00125"
     CDS             1082..1657
                     /locus_tag="VFL84_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607052.1"
                     /translation="MRNKPLSTGWAIFWFAIASGVFFQLVSIRYGPDEEARVAEPIEV
                     VVPSSLEEDVRAAQNLLAGDPDSALRHIERALERGNEASPELRALLQTWAANMQARRW
                     HWHRARAALSSAVSLAPNDERRRALAATEEACRNRPIKSCSWRTRRRTRAPFPRCAGA
                     AASATGRSRSSTSAAVVRGRRDRDRDVLAAA"
     gene            complement(1659..>2369)
                     /locus_tag="VFL84_00130"
     CDS             complement(1659..>2369)
                     /locus_tag="VFL84_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607053.1"
                     /translation="AVAAQAERRAAFRAKYPPTTPLIVGQLEEIRIVEDDNELYAADS
                     YLGGRVLVFALDTLAFKRGWGAYGKPLAEISVDDADHHYTPNGPMPRDFVGHLTINVS
                     RDGLVYAADRNANRIHVTRKDGTFVAEWLLAPGTGVGGSTGGVAFSPDREQRLLYVSD
                     LTNNRIWFLNRADGRVVGEMGRMGENGGQFFGLHMIAVDSQGNVYTGEVFQGERVQRF
                     VPADGAKGRLLERLPDTP"
BASE COUNT          352 a          798 c          844 g          375 t
ORIGIN      
        1 ccgcacgccg tcctgttcga gatgctgttc ggcgcgtgga ccgcgaagat cctcgcggag
       61 gtcgtgcgcc tcgacgttcc ggacgcgcta gcgcgaggcc cgctgacggc ggacgagctc
      121 gtccgcaagg ccggcatccg cgcccatccc ggcgcgctgc gtcgcgcgct gcgcgcgtgc
      181 gcggcgctcg gtatcgtgac cgaggactcc gcaggacggt tcgggccgac gcgcttgtcg
      241 gcgctgctga cgcgcgacac gccgggcacg ctgaagcgct tcgtcgagta ttcgggcggc
      301 acgctctgga aggtctggac gggcctgccg gaagcgctcg cgacgggcca ctcgcaagct
      361 cgcgcgcagc tcggcttgga cttcttcgac tatctcggcg ccaacccgca agcgctcgag
      421 gaattcggcg agacgctgaa ggcgcacagc gccgtcgcca acatcggcgt gctcgagcgc
      481 tacgacttcg gcggcatccg cacgctcgtc gacgtcggcg ccgggtacgg cccgctcacg
      541 atcgccgtgc tcgagaaata cgccgagatt cgcggcgtcg tgttcgactt gcccgaggtc
      601 atcgaggcgg cgccgagcag cttgcccgtc aagagcgatg ccgtcgcccg gcgcctgacc
      661 tacacggccg gcgacatgtt cgaagaagtt ccgccggccg acgcgtacgt gctcaagctg
      721 atcatgcacg actgggacga tgcgtcgtgc gcgaagatac tcgcgaacgt ttgcgcgcgg
      781 ctcgcgcgcg gcgggcgcgt catctgcatc gacaacgtga tgccgccgct cggcgacgtt
      841 tccgacgtcc cgcagaagct gctcgacgtg aacatgatgc tgctcctgcc cggcaaagag
      901 cgcacacgcg tcgagtggga ggcgctctac cgcggcgccg gattgtcgat ctcggcgatg
      961 atccccgttc cagacgcgtt caacatgttc gtcatcgagg ggcggcgcgc cggcgaggcc
     1021 tgaagcatac ggcgagcggc ggatcgcgta tcctcgccgc agcagtcgcg gggggcgact
     1081 cgtgcgcaac aaaccgctgt cgacgggatg ggccattttc tggttcgcga tcgcgagcgg
     1141 cgtctttttc cagctcgtgt cgatccggta cgggccggac gaggaagcgc gcgtcgccga
     1201 gccgatcgag gtcgtcgtgc catcctcgct cgaggaggat gtccgcgcgg cgcagaacct
     1261 gctcgccggc gatccggatt cggcgttgag acacatcgag cgcgcgctgg aacgggggaa
     1321 cgaggcgagc ccggagctgc gcgcgttgct gcaaacgtgg gcggccaaca tgcaggcgcg
     1381 ccgctggcac tggcatcgcg cccgtgcggc gctgtcgagc gcggtgagcc tcgcgccgaa
     1441 cgacgaacgc cggcgcgcgc tcgcggccac cgaagaggcg tgcaggaatc ggccgatcaa
     1501 gtcatgctcg tggcgcactc gccgcagaac gcgcgctcct tttcctcgat gtgccggcgc
     1561 ggcggcgagt gcgactggga gatctcgctc gtctacgagc gctgctgttg ttcgtggccg
     1621 tcgtgatcgc gaccgtgatg tcctagccgc cgcgtgagct acggcgtgtc cggcaaccgc
     1681 tcgagcagcc ggcctttcgc gccgtcggcg ggcacgaagc gctgcacgcg ctcgccctgg
     1741 aacacctcgc ccgtgtagac gttgccttgc gagtcgacgg cgatcatgtg caggccgaag
     1801 aactgaccgc cgttctcgcc catgcggccc atttcgccga cgacgcggcc gtcggcgcga
     1861 ttcaagaacc agatgcggtt gttcgtgaga tcggagacgt agagcaggcg ctgctcgcgg
     1921 tcgggcgaga acgcgacgcc gcccgtcgag ccgcccacgc ccgtgccggg cgcgagcagc
     1981 cactccgcga cgaacgtgcc gtccttgcgc gtcacgtgaa tgcgattcgc gttgcgatcg
     2041 gccgcgtaga cgagcccgtc gcgcgatacg ttgatcgtga gatggccgac gaagtcgcgc
     2101 ggcatggggc cgttcggcgt gtaatggtgg tcggcgtcgt cgacgctgat ctcggcgagc
     2161 ggcttgccgt aggcgcccca accgcgcttg aacgcgagcg tgtcgagcgc gaacacgagc
     2221 acgcggccgc ccaagtagct gtcggcggcg tagagctcgt tgtcgtcctc gacgatgcgg
     2281 atctcctcga gctgaccgac gatcagcggc gtcgtgggcg gatacttcgc gcggaacgcg
     2341 gcgcggcgct cggcctgcgc cgcgacggc
//
LOCUS       DASSNN010000008         4549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_253643, whole genome shotgun sequence.
ACCESSION   DASSNN010000008 DASSNN010000000
VERSION     DASSNN010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253643"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..975)
                     /gene="xerC"
                     /locus_tag="VFL84_00135"
     CDS             complement(76..975)
                     /gene="xerC"
                     /locus_tag="VFL84_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HET7607054.1"
                     /translation="MGRDELASFLKRLKTERRLSPHTCDAYERDVEALLAFCAREQIA
                     SLKELDSFGVRRFAAESHRKGLSARSVARRLSAVRTFLNYLIESGVVKSNAGVHVQAP
                     KAPRRLPATLDADQVASLLAISGDDPMTLRDRAILELFYSSGLRLAELVNLDLGDVDA
                     ADRTVRVVGKGSKVRVVPVGKHALAALRDWLAVRAGLARSGELALFVGRRGLRVSPRT
                     VQRQVNEWAKRRGAPTGVHPHMLRHSFATHLLESSGNLRAVQELLGHASLSTTQMYTH
                     VDFQHLAKVYDQAHPRARKRPAE"
     gene            complement(978..1688)
                     /locus_tag="VFL84_00140"
     CDS             complement(978..1688)
                     /locus_tag="VFL84_00140"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HET7607055.1"
                     /translation="MSQQQSGAAVGAPLIETASEDSIADYLKSHPDFFERHTPLVLGL
                     KLPHRAGGATISLVERQVSLLRQRNAQLERQLKDLVAVAKQNDALVEKIHQLCLKLMR
                     APTLLQRLERLETGLREDFAAERAVLVLFPAAPAEAVRDGFVKRANPDDADVRPFAAF
                     LRAAKPRCGPLRDRQKNIFDRDADAVSSAALVPLGAESRLGFLIIGSRDPDHFHPGKR
                     MDFLARLGEVLAVAIRNE"
     gene            complement(1685..2506)
                     /gene="dapF"
                     /locus_tag="VFL84_00145"
     CDS             complement(1685..2506)
                     /gene="dapF"
                     /locus_tag="VFL84_00145"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET7607056.1"
                     /translation="MKLAFTKMNGLGNDFMVVEWPSGRPPPEPELIRRWADRRRGVGF
                     DQLLLVTGERANGADASYRNFNADGGEVEQCGNGVRCLARFLAPRMGSELTLRSPAGA
                     VTARVRSPEDVSVNLGVPDFRPSALPFLVEQERDRYRVEVAGETVELGAVSMGNPHVV
                     IAVPSVAAAPVERLGAAFQRHQSFPRRVNVGFMERVDEQRIRLRVYERGVGETLACGT
                     GAAAAVAVGRRWGQLRERVAVELPGGTLEVNWSGPGTPLWQTGPTAAVYEGYIDI"
     gene            2661..3308
                     /locus_tag="VFL84_00150"
     CDS             2661..3308
                     /locus_tag="VFL84_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET7607057.1"
                     /translation="MRHILVTNAKGGCGKSTIATSLAAYFASEGYATALADFDPQASA
                     LSWLEQRPADCAPIKAVRGFESGLRTVPHDTEFVVIDSPAGSYGEPLRELVRRAETIV
                     VPVLPSPIDIKAAERFIDELLNISKVADKKAKAALVANRVRENTLIFEELDDVLSRLK
                     VPYVAQFREAQNYNRAYQRGLGVHELPPYLAWPDWEQWDPLIEWLDSRRSQPKAG"
     gene            complement(3305..>4549)
                     /locus_tag="VFL84_00155"
     CDS             complement(3305..>4549)
                     /locus_tag="VFL84_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HET7607058.1"
                     /translation="LARAAQRDAVMRFGVGIVLAVLLAAFGAHFLLADRGYVLVNFHE
                     LVIEMSVPGLIIVLVLAYLLVRALAALARAPRRWRASRRQRKLERRESDLTAGIAQLV
                     EGNWARSERTLTKGLKDADAPLANCLLAAHAAELQGAADRRDQWLDLAQGIGESAVAS
                     AALTRAELQLKAGDAAAAAATLTAMKPEDAAQPAAIALLARAYRALGRSEQLFALLPQ
                     LSRAALAPAEREQLETLAVQGELGRGDLTAERLAEAWAAVPSELRAAPAIVAQRARAL
                     ARLRCGEQAERELRGALKRDWHAALVEAYGEVRGADPAKQLKQAETWLKSHPDDAALL
                     GALARLSLANELWGKARSYLEARLAIAPEPEAYALYGQLLTQLGEDEAALAAFRSGLQ
                     LVTSAAAEPLPPAREPERKAQG"
BASE COUNT          687 a         1610 c         1572 g          680 t
ORIGIN      
        1 gcagacgatc gtcgtgccgt ggaagttgct cattggccga ttttaagcca gccgcgcgcg
       61 cggcgccgct tacggctact cggccgggcg cttgcgcgcg cgcggatggg cttggtcgta
      121 gaccttggcg aggtgctgga agtcgacgtg cgtgtacatc tgcgtcgtcg acaggctcgc
      181 gtggccgagc agctcctgca cggcgcgcag gttgccgctc gactcgagca ggtgcgtcgc
      241 gaacgagtgg cgcagcatgt gcgggtgcac gcccgtcggg gcgccgcgcc gcttcgccca
      301 ctcgttgacc tgtctctgca cggtgcgggg gctcacgcgc agcccgcggc ggccgacgaa
      361 cagcgcgagc tcgccgctgc gcgcgagccc cgctcgcacg gcgagccagt cgcgcagggc
      421 cgcgagcgcg tgcttgccga ccggcacgac gcggaccttc gagcccttgc cgacgactcg
      481 cacggtgcgg tcggccgcgt cgacgtcgcc caaatcgaga ttgacgagct ccgcgagccg
      541 caagccggac gagtagaaca gctcgaggat cgcccggtcg cgcagcgtca tcgggtcgtc
      601 cccgctgatc gcgagcaggc tcgcgacttg atcggcgtcg agcgtcgccg gcaaccggcg
      661 cggcgccttc ggtgcctgga cgtgaactcc cgcgttgctc ttcacgactc cggactcgat
      721 caggtaattc aggaacgtgc gcacggccga cagccgccgc gcgacgctgc gcgccgagag
      781 ccctttgcga tggctctcgg ccgcgaaccg gcgcacgccg aacgaatcga gctccttcag
      841 cgacgcgatc tgctcgcgcg cacagaacgc gagcaacgct tcgacgtctc gctcgtacgc
      901 atcgcacgtg tgcggcgaca gccgcctctc ggtcttgagc cgcttcaaga acgaggcgag
      961 ctcgtctcga cccacgccta ttcgttgcga atggcgacgg ccagcacttc gcccaagcgc
     1021 gcgaggaaat ccattcgctt gcccggatga aagtgatcgg gatcgcggct gccgatgatc
     1081 aggaagccga gccgcgactc cgcgccgagc ggcacgagcg ccgcgctgct cacggcgtcg
     1141 gcgtcgcggt cgaagatgtt cttctgccgg tcgcggagcg gtccgcagcg cggcttcgcg
     1201 gcgcgcagga acgcggcgaa cggccgaacg tcggcatcgt cgggattcgc gcgcttgacg
     1261 aagccgtcgc gcacggcctc ggccggtgcg gcggggaaca gcacgagcac ggcgcgctcg
     1321 gccgcgaagt cctcgcgaag ccccgtctcg agacgctcga gccgctgcag cagggtcggc
     1381 gcgcgcatga gcttcaggca gagctgatga atcttctcga cgagcgcgtc gttttgcttc
     1441 gcgacggcga cgagatcctt gagctggcgc tcgagctgcg cgttgcgttg ccgcagcagc
     1501 gagacttgcc gttcgacgag cgagatcgtg gccccgcccg cgcgatgagg cagcttgagc
     1561 ccgagcacca acggggtgtg gcgctcgaaa aaatcggggt gactcttcag atagtcggcg
     1621 atcgagtctt cggaagccgt ctcgatcaat ggagcgccga cggccgcccc gctctgctgc
     1681 tgactcatat gtcgatgtaa ccctcgtaca ccgctgccgt aggtccagtt tgccacagcg
     1741 gcgtgccggg tccagaccaa ttcacttcga gcgtgccgcc cggcagctcg accgcgactc
     1801 gctcgcgaag ctgtccccag cggcggccga ccgcgacggc cgcggccgca ccggtgccgc
     1861 aggcgagcgt ctcgccgacg ccgcgctcgt aaacgcgcag ccgaatgcgc tgctcgtcga
     1921 cgcgctccat gaaaccgacg ttgacgcgcc gcggaaagct ttgatggcgc tgaaacgcgg
     1981 cgccgaggcg ctcgaccggc gcggcggcga cggacggcac ggcgatgacg acgtgcggat
     2041 tccccatcga gaccgcgccc agctcgaccg tctcgcccgc gacctcgacg cgatagcgat
     2101 cgcgctcttg ctcgaccagg aacggcagag cgctcgggcg aaagtcgggc acgcccaagt
     2161 tgacgctgac gtcctcgggc gagcgcacgc gagccgtgac ggcgccggcg gggcttcgga
     2221 gcgtgagctc gctccccata cgcggcgcga ggaaccgcgc gaggcagcgc acaccgttgc
     2281 cgcattgctc gacctcgccg ccgtcggcgt tgaagtttcg gtagctcgcg tcggcgccgt
     2341 tcgcgcgctc gcccgtcacg agcagcagct ggtcgaaccc gacgccgcga cgccggtcgg
     2401 cccagcgccg gatcagctcg ggctccggcg gcgggcgccc cgacggccat tcgacgacca
     2461 tgaagtcgtt gccgaggcca ttcatcttgg tgaaagcgag cttcatggcg gtgcgtgatc
     2521 ggcggcggtc gagggcggcg gacggcgctt ggaacgcgcg tatcttaggc gaaaaggccg
     2581 aactcgacat ttgtcataag cctggctaag ttacgcccat ttcccccgcg cgccagaaca
     2641 acaactcgag cggagtgccc atgcgacaca ttctcgtcac caacgcgaag ggcggctgcg
     2701 gcaagagcac gattgccacg agcctcgcgg cctacttcgc gtccgaaggt tatgcgacgg
     2761 cgctcgccga cttcgacccg caggcgtccg cgctgtcgtg gctcgagcag cgccccgccg
     2821 actgcgcgcc gatcaaggcc gtgcgcggct tcgagtcggg tttgcgtacc gtgccgcacg
     2881 acacggagtt cgtcgtcatc gattcgccgg cgggctccta cggcgagcct ttgcgggagc
     2941 tcgtgcgcag ggccgagacg atcgtcgtgc ccgtgctgcc ttcgccgatc gacatcaagg
     3001 ccgcggagcg gttcatcgac gagctgctga atatctcgaa ggttgcggac aagaaagcga
     3061 aggcggcgct cgtcgcgaac cgcgtccgcg agaacacgct gatcttcgag gagctcgacg
     3121 acgtgctgtc gcggctcaaa gtgccgtacg tcgcgcagtt ccgcgaggcc cagaactaca
     3181 accgcgcgta tcagcgcggc ctcggcgtgc acgagctgcc gccgtatctc gcctggccgg
     3241 actgggagca gtgggatccg ttgatcgagt ggctcgattc gcgccgctcg cagccgaagg
     3301 ccggctagcc ctgcgcttta cgctcgggtt cgcgcgccgg cggcagcggc tcggccgcgg
     3361 ctgaagtcac gagctgaagc cccgaccgaa acgccgcgag cgcggcctcg tcctcgccga
     3421 gctgcgtcag gagctggccg tacagcgcgt aggcctcggg ctccggggcg atcgccaagc
     3481 gcgcctccag atagctgcgc gccttgcccc agagctcgtt cgcgagcgag agacgcgcga
     3541 gcgccccgag cagcgccgcg tcgtcgggat gggactttag ccacgtctct gcctgcttga
     3601 gctgcttcgc cgggtcggcg ccgcgaacct cgccgtaagc ttcgacgagc gccgcgtgcc
     3661 agtcgcgctt cagcgcgccg cgcagctcgc gctcggcttg ctcgccgcat ctcaagcgcg
     3721 cgagcgcccg ggcgcgttgc gcgacgatcg ccggcgccgc acgcagctcg ctcggcaccg
     3781 ccgcccacgc ttcggcgagc cgctcggccg tcaagtcgcc gcgaccgagc tcaccctgca
     3841 cggccagcgt ctcgagctgc tcgcgctccg cgggcgcgag cgccgcccgc gaaagttgcg
     3901 gcagcagcgc gaacagctgc tcgctgcgcc cgagcgcgcg ataggcgcgc gcgagcagcg
     3961 caatcgcggc cggttgcgcc gcgtcctcgg gcttcatggc cgtgagcgtc gcggccgctg
     4021 ccgcggcatc gccggctttg agctgaagct ctgcgcgcgt gagcgccgcg ctcgcgaccg
     4081 cgctctcgcc gatgccttgc gcgagatcga gccattggtc gcgccggtcc gccgctccct
     4141 gaagctctgc agcgtgcgcg gcgagcaagc agttcgcgag cggcgcgtcc gcatccttca
     4201 accccttcgt caaggtccgt tcgctgcgcg cccagttgcc ctcgacgagc tgcgcgatgc
     4261 cggccgtcag atcgctctcg cggcgttcga gcttgcgttg ccgccgcgac gcgcgccagc
     4321 gtcgcggcgc tcgcgcgagc gcggcgagcg cgcgcacgag caggtacgcc agcacgagca
     4381 cgatgatgag tcccggcacc gacatctcga tgacgagctc gtggaagttc acgagcacgt
     4441 agcctcgatc ggcgagcaag aagtgcgcgc cgaatgcggc gagcagcacc gcgaggacta
     4501 tcccgacacc gaagcgcatc accgcgtctc gttgcgctgc tcgcgcaag
//
LOCUS       DASSNN010000009         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_380159, whole genome shotgun sequence.
ACCESSION   DASSNN010000009 DASSNN010000000
VERSION     DASSNN010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380159"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1471
                     /locus_tag="VFL84_00160"
     CDS             146..1471
                     /locus_tag="VFL84_00160"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7607059.1"
                     /translation="MSNELPVADRRLDPAEMESRFQGLVQSPLRAAILRFLCARPDDA
                     FDVESLMQTFGRLRLDVENCVRELVAFGVASRSSSGQGSPKYRFVPPVNDVLRELLDS
                     FLERRATVSHEDRSPAVQRFREMIGRDEKMLVIFEWIRTAAKSDISVLILGPTGSGKE
                     VVARLIHELSRRGTNKFQAVNCAALPDTLFESEIFGYEKGAFTGAHDRKQGRLELANN
                     GTLFLDEIGDMSIVAQAKLLRVLEERRFERLGGNTSIEVNFRLISATNRPLEQFVRDS
                     RFREDLYYRVNAFSIRLPSLRERATDIPVLAQRFLARYCAANGLPLDGKALSKEAADL
                     LMQYHWPGNIRELESTVSRAALSSPGRVIRATDIEFLHAPEAPSEASSDRLPSLAQAE
                     RAHVLRVLESVQWNKKQAAEILEISRGTLYRKIVEYGLETEPKPVVKAR"
     gene            1692..>2282
                     /locus_tag="VFL84_00165"
     CDS             1692..>2282
                     /locus_tag="VFL84_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7607060.1"
                     /translation="MLRRLFLPALLAVLCVPAHADLAASPLDGIVRAVTVDGASSASA
                     ERVSAAVGADWVRQSLGYDGSGVTIAVIDSGITDAHDDLSSSHVARFADFVNFRAQPY
                     DDYGHGTHVAGILAGDGFDSSGTRRGIAPGVSLLVEKVLDETGQGYISNVIAAIDYAI
                     ANRDALGIRIINLSVAASVAESYMNDPLALAAKRAVD"
BASE COUNT          394 a          774 c          742 g          372 t
ORIGIN      
        1 tcgaacgtcg tcatcgagac gagcggcggg tcggacccgg tgctgtgcca tggccaccgt
       61 aagcgtgacg cgatcgagat gaagaaactg atcaaccagt accagacggc ctattacggc
      121 aagggacaga caccggcctg agcgcatgtc gaacgagctg cccgtcgccg accgccgtct
      181 cgatcccgcc gaaatggagt cccgcttcca ggggctcgtc cagtcgccgc tgcgcgccgc
      241 gattctccgg ttcctctgcg cgcggcccga cgacgcgttc gacgtcgagt cgctgatgca
      301 gacgttcggg cgcctgcgcc tcgacgtcga gaactgcgtg cgcgagctgg tcgcgttcgg
      361 cgtcgcgagc cgatcgagca gcggacaggg gtcgccgaaa taccggttcg tgccgccggt
      421 caacgacgtg ctgcgcgagc tgctcgattc gttcctcgag cggcgcgcca cggtgagcca
      481 cgaggatcga tcgccggccg tccagcgctt ccgcgagatg atcgggcgcg acgagaagat
      541 gctcgtcatc ttcgagtgga tccgcaccgc cgcgaagtcg gacatttcgg tgctgattct
      601 cgggccgacc ggctcgggca aggaagtcgt cgcgcggctg attcacgaac tcagccggcg
      661 cggcacgaac aagttccagg cggtcaattg cgcggcgctt cccgacacgc tcttcgaatc
      721 ggaaatcttc ggctacgaga agggcgcgtt caccggcgcg cacgaccgca agcaggggcg
      781 gctcgagctc gccaacaacg ggacgctgtt cctcgacgag attggcgaca tgtcgatcgt
      841 ggcgcaagcc aagctgctgc gcgtcctcga agagcggcgg ttcgaacggc tcggcggcaa
      901 cacgtcgatc gaggtgaact tccggttgat ctccgcgacg aaccggccgc tcgagcagtt
      961 cgtccgcgac agccgcttcc gcgaagacct ctactaccgc gtgaacgcgt tctcgatccg
     1021 cctgccgtcg ctccgcgagc gggcgaccga cattccggtg ctggcgcagc ggttcctcgc
     1081 gcgctactgc gccgccaacg ggctgccgct cgacggaaag gcgttgtcga aagaggccgc
     1141 ggatctgctc atgcagtatc actggccggg gaacatccgc gagctcgaaa gcacggtgtc
     1201 ccgcgcggcg ctgtcgtcgc cgggccgcgt gattcgcgcc accgatatcg aattcctgca
     1261 cgcgccggag gcgccctccg aggcctcgtc cgaccggctg ccgtcgctcg cgcaagccga
     1321 acgcgcacac gtgctgcgcg tgctcgagag cgtgcagtgg aacaagaaac aggcggcgga
     1381 gattctcgag atcagccgcg gaacgctcta tcgcaagatc gtcgaatacg ggctcgagac
     1441 agagccaaaa cccgtagtga aagcgcgctg acgcgaatgg ccacggcggt ggcacctctt
     1501 cagcaactga ccttttcagc cgatacatgt cacggaacgg aacacggctg gtagatcggg
     1561 acaaaaacca ccagaattct gccttgagga agacgcgtaa gatcatccgg atcagcgatt
     1621 taggcagcaa aacgcccacc tcatccggcg gcagcctgct tgcaccggcg gagggctctg
     1681 gccggctccg tatgcttcga cgccttttcc ttccggcgct gctcgcggtc ttgtgcgttc
     1741 ccgcacacgc ggatctcgcc gcgtctccgc tcgacggcat cgtccgcgcg gtcactgtcg
     1801 acggcgcttc gtccgcgtcc gcggaacgcg tgtcggcggc ggtcggcgcg gactgggtgc
     1861 ggcagtcgct cggctacgac ggctcgggcg tgacgatcgc cgtcatcgat tcgggcatca
     1921 cggacgcgca cgacgatctc tcgtcgtccc acgtcgcgcg ctttgccgac ttcgtcaatt
     1981 tccgcgcgca gccgtacgac gactacggcc acggcacgca cgtggcgggc atcctcgcgg
     2041 gcgacgggtt cgactcatcg ggcacgcgcc gcggcatcgc gccgggcgtc tcgctgctcg
     2101 tcgagaaggt gctggacgaa accggccagg gttacatcag caacgtgatc gcggcgatcg
     2161 actacgccat cgccaaccgc gacgcgctcg gcatccggat catcaacctg tccgtcgccg
     2221 catccgtcgc cgagtcctac atgaacgacc cgctcgcgct tgccgcgaag cgcgccgtgg
     2281 ac
//
LOCUS       DASSNN010000010         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_128465, whole genome shotgun sequence.
ACCESSION   DASSNN010000010 DASSNN010000000
VERSION     DASSNN010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128465"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..2085
                     /locus_tag="VFL84_00170"
     CDS             412..2085
                     /locus_tag="VFL84_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET7607061.1"
                     /translation="MRPESPRSRRERGPKGRVVDDAALADVRALLGARPRRRDLLIEH
                     LHLIQDKFGCLEARHLRALAEEMRLSQAEVYEVASFYDHFDVVKEDEPKPAPVTVRVC
                     ESISCMIAGADELIETLSAGVDPKQVRVVRAACIGRCAGAPAARVGDREVENATVEGL
                     LRDVRERRTGVVVPEYVALAEYRKNGGYALYESVRKGELTAEAIIAKLQSSGLRGLGG
                     AGFPPGKKWQFVRSYAGPRLMSINGDEGEPGTFKDRWFLERDPHRTLEGALIAAHAVE
                     AQRIYFYMRNEYPGVLEILRREIAALEVAGIAAPGFIELRRGAGAYICGEESAMIESI
                     EGKRGLPRNRPPYIAEVGIFGRPTLNQNVETLWWIRDILEKGPEWFAKQGREGHPGPR
                     SWSVSGRVKKPGVVQAPAGVTVRELIDDYCGGMLDGHEFTAYLPGGASGGILPASMGD
                     IPLDFGGELAKIGAFVGSHAVVVFSDQDDVKEAVINLLKFFRHESCGQCTPCREGTDK
                     MVGLLAAKGALNEPALRDLEAVMRDSSICGLGQAAPNPVAHLLTLFRKD"
     gene            2092..>3470
                     /locus_tag="VFL84_00175"
     CDS             2092..>3470
                     /locus_tag="VFL84_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET7607062.1"
                     /translation="MTDSIRFKLDGKEVAARAGETIWDVAKREGTKIPHLCHVDLPGY
                     RVDGNCRACMVEVKGERVLTASCIRKPTAGMEVATQTERATKSRKLVFELLASNMRPR
                     EQGPDNQAPFWKWAESMGIKGSDRFGSKFNDGHHEPGLDLTNPAIAVNLDACISCGAC
                     VRACREVQVNDVIGMGLRGSHAVPVFDIHDPMGLSTCVTCGECVQACPTGALYEKSLM
                     DKTATKRAVQKFDKVVNTLCPFCGVGCQTAVAVKDNRIVQVDGRNGYANENRLCVKGR
                     FGYDYVSSPERLTKPLVRRDDAPKVDSPETMRYEDRFSVFREATWEEALTRAAAGIKK
                     ILDERGGKALSGFGSAKGSNEEAYLFQKLVRTGFETNNVDHCTRLCHASSVAALMEGI
                     GSGAVSAPFNAAMDAECVIVIGARPTVNHPVAATFFKQAAKRGAKLIVMDPRGQDLMH
                     HATHALKFKP"
BASE COUNT          587 a         1137 c         1206 g          540 t
ORIGIN      
        1 cgtcgccctc ggcggccgcg actgactcga ttgtcaggaa ggcggcgagc gcgacggcga
       61 atcgcgcggc agtgctgaca atcgggcgca ggcgccgcgt cacggcgggt ctccggcggc
      121 tgacgacgac catgcgcagc ctatctcacg gcaacctgtt gcgcgcggcc cgctggcgtc
      181 gatcggtgca tagtaaggac aaacggcgcg caatggactt cattgctcgg cgaaacagct
      241 ggattcccga tacccagcgg gttccgcgcg agggcgccgg gctggtatcc tcaccggcgg
      301 atagcgacgg cgcgctcggg ctcgccgaac cgggacagat cgttgattgt tgttacatat
      361 gcctctctgc tctgacggac ccgcgcaatc gagcagggag agggattcca aatgagacct
      421 gaatctccgc gctcgcgccg cgagcgcggg ccgaaaggcc gcgtcgtcga cgacgcggcg
      481 ctcgcagacg ttcgcgcgtt gctgggcgcg cgcccgcgcc gtcgcgacct gctgatcgag
      541 cacctgcacc tgatccagga caaattcggc tgcctcgagg cgcggcactt gcgcgcgctg
      601 gccgaggaaa tgcgcttgtc gcaggccgag gtctacgagg tcgcttcgtt ctacgaccac
      661 ttcgacgtcg tcaaagagga cgagccgaag ccggcgcccg tgacggtgcg cgtctgcgag
      721 tcgatcagct gcatgatcgc cggcgccgac gagctcatcg aaaccttgag cgccggcgtc
      781 gatccgaagc aagtccgcgt cgtgcgggcc gcctgcatcg gtcgctgcgc gggcgcgccg
      841 gccgcgcgcg tcggcgaccg cgaggtcgag aacgcgacgg tcgaggggtt gctgcgcgac
      901 gtgcgcgagc ggcgcaccgg cgtcgtcgtg ccggagtacg tcgcgctcgc cgagtaccgc
      961 aagaacggcg gctacgcgct ctacgagtcc gttcgcaaag gcgagctcac ggccgaggcg
     1021 atcatcgcga agctccagtc gtcgggcctg cgcggcctcg gcggcgccgg cttcccgccc
     1081 ggcaagaagt ggcagttcgt gcgctcgtac gcggggccgc gcctcatgtc gatcaacggc
     1141 gacgagggcg agcccgggac cttcaaggac cgctggttct tggagcgcga cccgcatcgc
     1201 acgctcgaag gcgcgttgat cgccgcgcac gccgtcgagg cgcagcgcat ctacttctat
     1261 atgcgcaacg agtatcccgg cgtgctcgag atcttgcggc gcgagatcgc cgcgctcgag
     1321 gtggccggca tcgctgcgcc cggtttcatc gagctgcgcc gcggcgcggg cgcgtacatc
     1381 tgcggcgaag agagcgcgat gatcgagagc atcgaaggca agcgcggctt gccgcgcaac
     1441 cggccgccgt acatcgccga ggtcggcatc ttcggccggc cgacgctgaa tcagaacgtc
     1501 gagacgctct ggtggatccg cgacatcctc gagaaaggcc ccgagtggtt cgcgaagcaa
     1561 gggcgcgaag gccatcccgg cccgcggtct tggtccgtgt cgggccgcgt caagaagccc
     1621 ggcgtcgttc aggcgccggc cggcgtcacg gtccgcgagc tcatcgacga ttactgcggc
     1681 ggcatgctcg acggccacga gttcacggcc tatctgcccg gcggcgcctc gggcggcatc
     1741 ctgcccgcgt cgatgggcga cattccgctc gatttcggcg gcgagctcgc caagatcggc
     1801 gcatttgtcg gctcgcatgc cgtcgtcgtg ttctcggacc aggacgacgt caaggaggcc
     1861 gtcatcaact tgctgaaatt cttcaggcac gaaagctgcg gccagtgcac gccttgccgc
     1921 gaaggcacgg ataaaatggt cgggctgctt gcggcgaaag gcgctttgaa cgaaccggcg
     1981 ctgcgcgatc tcgaggccgt catgcgcgat tcgtcgatct gtggcctcgg ccaagccgcg
     2041 ccgaaccccg tcgcccactt gctgacgctg tttcgcaagg actgaagtga aatgaccgat
     2101 tcgattcgtt tcaagctcga cggcaaagag gttgccgcgc gcgccggcga gaccatctgg
     2161 gacgtcgcga agcgcgaagg cacgaagatt ccgcatcttt gccacgtcga cctgccgggt
     2221 tatcgcgtcg acggcaactg ccgcgcctgc atggtcgagg tcaaaggcga gcgcgtgctg
     2281 acggcgtcgt gcatccgcaa gccgacggcc ggcatggagg tcgcgacgca gacggagcgc
     2341 gcgacgaagt cgcggaagtt ggtgttcgag ctgctcgcga gcaacatgcg gccgcgcgag
     2401 cagggccccg acaatcaggc gccgttctgg aagtgggccg agtcgatggg catcaagggc
     2461 agcgaccgct tcggctcgaa gttcaacgac ggtcaccacg agcctggcct cgatctcacg
     2521 aatcccgcga ttgcggtcaa tctcgacgcg tgcatctcgt gcggcgcgtg cgtgcgcgcg
     2581 tgtcgtgagg tgcaagtcaa cgacgtcatc ggcatgggac tgcgcggcag ccacgccgtg
     2641 cccgtgttcg acatccacga tcccatgggc ctctcgacgt gcgtgacgtg cggcgagtgc
     2701 gtgcaggcgt gcccgacggg cgcgctgtac gagaagtcgc tgatggacaa gacggcgacg
     2761 aagcgcgccg tgcagaagtt cgacaaggtc gtcaacacgc tgtgcccgtt ctgcggcgtc
     2821 ggctgccaga cggccgtggc cgtcaaggac aaccgcatcg tgcaggtcga cggccgcaac
     2881 ggctacgcga acgagaaccg gctctgcgtc aaagggcggt tcggctacga ctacgtgtct
     2941 tcgcccgagc gtttgacgaa gccgctcgtg cggcgcgacg acgcgccgaa agtcgattcg
     3001 ccggagacga tgcgttacga agatcgcttc agcgtcttcc gcgaggcgac ctgggaagaa
     3061 gcgctgacgc gggcggcggc gggcatcaag aagatcctcg acgagcgcgg cggcaaagcg
     3121 ttgtccggct tcggctcggc gaaaggctcg aacgaggagg cgtacctgtt ccagaagctc
     3181 gtgcgcacgg gcttcgagac gaacaacgtc gatcactgca cgcggctctg ccacgcgtcg
     3241 tccgtcgccg cgctcatgga aggcatcggc tccggcgccg tgtccgcgcc gttcaacgcc
     3301 gcgatggatg ccgagtgcgt catcgtcatc ggcgcgcgcc cgaccgtgaa ccacccggtc
     3361 gccgcgacgt tcttcaagca ggccgcgaag cgcggcgcga agctcatcgt catggatccg
     3421 cgcggccagg acctcatgca ccacgcgacg cacgcgctga agttcaagcc
//
LOCUS       DASSNN010000011         4455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_883991, whole genome shotgun sequence.
ACCESSION   DASSNN010000011 DASSNN010000000
VERSION     DASSNN010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883991"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1258)
                     /gene="aroC"
                     /locus_tag="VFL84_00180"
     CDS             complement(155..1258)
                     /gene="aroC"
                     /locus_tag="VFL84_00180"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET7607063.1"
                     /translation="MAGDTYGRLFTVTTFGESHGPAMGCVVDGCPPGLALTEADIQKD
                     LDRRRPGRSRHVTQRQEPDQVKILSGVFEGRTTGTAIGLLVENVDQKSGDYSKIKDSF
                     RPGHADYTYQQKYGIRDYRGGGRSSARETVMRVAAGAIARKYLDEQLRLKIRGYLAEI
                     GPFKLRALDLEHVDENPFFCADPDKLPQLEVFLDELRRAGDSIGARINLIASGVPVGL
                     GEPVFDKLEGEIAKGLMSINAVKGVEFGDGFECVRQRGSEHRDEMTPDGFKKNSSGGT
                     LGGISSGQDILVSIALKPTSSIQVPGSTVDKEGRATEIVTTGRHDPCVGLRATPIAEA
                     MLALTLIDHYLRHRAQNADVRSSVPVLAARKGP"
     gene            complement(1330..2235)
                     /gene="prmB"
                     /locus_tag="VFL84_00185"
     CDS             complement(1330..2235)
                     /gene="prmB"
                     /locus_tag="VFL84_00185"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018949924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET7607064.1"
                     /translation="MGTPSLERRLRAARDCDEWIAALADYFAGHGLAFGHGTDNAGDE
                     AFWLLRHLQGWGDADFAAPPAPDVTARAIAIAERRVRDRKPLAYLLNEAWFADLKFYV
                     DERVLVPRSPLAEVVERGFAPWVKLARGDRVLDIGTGSGCIAIAAAHYCPDVEVHATD
                     ISAAALAVAARNVERHGLEGRVRLFEADLFPPLDERYRVIVSNPPYVPEREVAALPPE
                     YRHEPVLGLASGADGFDAAERILRGARDRLTPDGALFIELGAGADAFAAAHRELPLIA
                     LECERGGDGVLATTAAEIREFLRTR"
     gene            2306..2950
                     /locus_tag="VFL84_00190"
     CDS             2306..2950
                     /locus_tag="VFL84_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET7607065.1"
                     /translation="MLGGMVRIVVVFLVVLVAAMFIFRGRHVAAPENATELPQPTPLA
                     DVRFVDKSGEETHLSDLRGDFTVVFFGFTNCPDVCPLTLAMLAQARADIASRAPRFTP
                     RVLFVSVDPERDTPEKIGAYLDRFDAAFLGVTAPDAELEPLLKQLGVAVEREHDHAGG
                     GSYNVVHNSAIYVLDANADWIAVSTGPHDPKVFASDYLRIRQRHAGGGSRPPGA"
     gene            2947..3789
                     /gene="asd"
                     /locus_tag="VFL84_00195"
     CDS             2947..3789
                     /gene="asd"
                     /locus_tag="VFL84_00195"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HET7607066.1"
                     /translation="MTSSNWIRLTQHLLPKNLSSAVVYRATRSRRPWLKKPLTRWFAK
                     AYGVDLTEAANADLESYATFNDFFTRELKPNARPLEGGPGTVTSPADGVLTEHGAIEG
                     GRLYQAKGSAYTLAELLGESGAAVAALEGGRYFTIYLAPHNYHRVHSPLDAQLTLTRY
                     IPGSRYSVSRATVAAIPRLFCRNERVVLRFETSFGPMIVVLVGALNVSSISTFAHGEI
                     ASGAARQWVEQPPLPVARGAEIGRFNMGSTVVVLLGSDRFRFSPDLSDGLAVRMGQAL
                     GSFA"
     gene            3799..>4455
                     /locus_tag="VFL84_00200"
     CDS             3799..>4455
                     /locus_tag="VFL84_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET7607067.1"
                     /translation="MSYERGASLETLRQRAAMLAAVRAFFAARGVLEVETPALSSAGA
                     TDPALESIAANARSLGSRQQYLQTSPEFAMKRLLAAGSGDIYQLCRVFRDDELGRWHQ
                     PEFMLLEWYRVGFDEQRLMSEVAELIAAALAAAAPGGASAAPRIVRLTYAQALEAALG
                     LLPDAPTAELVRALAKQGIDVPANLSHDAVLDLAFGTAVLASFDGTAVTFVYDYPASQ
                     A"
BASE COUNT          660 a         1567 c         1529 g          699 t
ORIGIN      
        1 ggaatttaac aatttacggc gcccgcgaac tgtgattctc gttcgacttt cgcgatggcg
       61 cgccgcgagg ccggcgcggt tcttttacca taatacccac gccggcgcgg ccctggcgag
      121 gctcgagggc gtcgcggccg cccggcggcg gccttcaggg gcctttgcgc gcggcgagga
      181 cgggcacgct cgaccgaacg tcggcgttct gggcccggtg gcgtaaataa tgatcgatca
      241 gcgtcagcgc gagcatcgcc tcggcaatcg gcgtcgcgcg cagcccgacg cacgggtcgt
      301 ggcgcccggt cgtcacgatc tcggtggcgc gcccttcctt gtccacggtc gagccgggca
      361 cctggatgct cgacgtcggc ttcagcgcga tgctgacgag aatgtcctgg ccgctcgaga
      421 taccgccgag cgtgccgccc gaagagttct tcttgaagcc gtcgggcgtc atctcgtcgc
      481 gatgctcact gccgcgctgg cgcacgcact cgaagccgtc gccgaactcg acgcccttga
      541 cggcgttgat gctcatgagc cctttcgcga tctcgccctc gagcttgtcg aacacgggct
      601 cgccgaggcc gacgggcact cccgaagcga tcaagttgat gcgcgcgccg atcgaatcgc
      661 ccgccctgcg cagctcgtcg aggaacacct cgagctgcgg cagcttgtcg ggatcggcgc
      721 agaagaacgg attctcgtcg acgtgctcga gatcgagcgc gcgcagcttg aacgggccga
      781 tctcggcgag ataaccgcgg atctttagcc taagctgctc gtcgagatac ttgcgcgcga
      841 tcgcgcccgc cgcgacgcgc atgacggtct cgcgcgcgga cgagcggccg ccgccgcggt
      901 aatcgcgaat gccgtacttc tgctgatacg tgtaatcggc gtgaccgggc ctaaagctgt
      961 ccttgatctt cgagtagtcg ccgctcttct gatcgacgtt ctcgacgagc aagccgatcg
     1021 cggtgccggt cgtacggcct tcgaacacgc ccgacaggat cttcacctgg tccggctcct
     1081 gccgctgcgt gacgtgccgc gagcggccgg ggcggcgccg atcgaggtcc ttttggatgt
     1141 cggcctcggt caacgcgaga cccggcgggc agccgtcgac gacgcagccc atcgcgggcc
     1201 cgtggctctc gccgaaggtc gtgaccgtga acagccgtcc gtacgtatcg cctgccatct
     1261 gccctgctcc actcgcgagc cggctcttcg gcccgccgcg cctcgcgccg gtgcgcacat
     1321 gatgcgcgct tagcgcgtgc gaaggaattc ccggatctcg gccgccgtcg tcgcgagcac
     1381 gccgtcgccg ccgcgctcgc actcgagggc aatcagcggc agctctcgat gcgcggccgc
     1441 gaacgcgtcg gcgccggcgc cgagctcgat gaacagcgcg ccgtccggag tcagccggtc
     1501 gcgcgcgccg cgcaggattc gctcggcggc gtcgaagccg tccgcgccgc tcgcgaggcc
     1561 gagaaccggc tcgtgccggt actcgggcgg caacgccgcg acctcgcgct cgggcacgta
     1621 gggcgggttc gacacgatca cgcggtagcg ctcgtcgagc ggcggaaaga gatccgcctc
     1681 gaacaggcgg acgcgacctt caaggccgtg gcgctcgacg ttgcgcgccg cgacggcgag
     1741 cgcggccgcg gaaatgtcgg tcgcgtgcac ctcgacgtcc gggcaatagt gcgcggcggc
     1801 gatcgcgatg cagccgctgc ccgtgccgat gtcgagcacg cggtcgccgc gcgcgagctt
     1861 cacccacgga gcgaagccgc gctcgacgac ttcggcgagc ggcgagcgcg gcacgagcac
     1921 gcgttcgtcg acgtagaatt tcaggtccgc gaaccaggct tcgttgagca aataggcgag
     1981 cggcttgcgg tcacggacgc gccgctccgc gatcgcgatc gcgcgcgcgg tcacgtccgg
     2041 cgccggcggc gcggcgaaat cggcgtcgcc ccacccctga aggtgacgca gcagccagaa
     2101 ggcctcgtcg ccggcattgt cggtgccgtg gccgaacgcg aggccgtgcc cggcgaagta
     2161 atccgcgagc gcggcgatcc actcgtcgca atcgcgcgcg gcgcgcaagc gccgctcgag
     2221 acttggggta cccatgcggc gcgcatgata acccttggac cttggcctgc ctccggcacc
     2281 cccgccggcg tgcgataatc gcaacgtgct gggcggaatg gtgcgcatcg tcgtcgtctt
     2341 cttggtcgtg ctcgtcgcgg ccatgttcat attccgcggt cgacacgtcg cggcgccgga
     2401 gaacgcgacc gagctgccgc agccgacacc gctcgcggac gttcgcttcg tggacaagtc
     2461 cggcgaggaa acgcacctga gcgacctgcg cggcgatttc acggtcgtgt tcttcggctt
     2521 cacgaattgc cccgacgtgt gtccgctcac gctcgcgatg ctcgcgcaag cgcgcgcgga
     2581 catcgcgagc cgcgcgccgc gcttcacgcc gcgcgtgctg ttcgtcagcg tcgatccgga
     2641 gcgcgacacg ccggagaaga tcggcgcgta cttggaccgc ttcgacgcgg cgtttctcgg
     2701 cgtcacggcg cccgacgccg agctcgagcc gctgctgaag cagctcggcg tcgcggtcga
     2761 gagagagcac gatcatgccg gcggcggcag ctacaacgtc gtgcacaact ccgcgatcta
     2821 cgtgctcgac gcgaacgcgg actggatcgc cgtgtcgacg ggaccgcacg atccgaaggt
     2881 cttcgcttcc gactatctac gcatccggca gcggcacgcc ggaggcggca gccgcccgcc
     2941 cggcgcatga cgtcgtccaa ctggatccgg ctgacgcaac acctgcttcc gaagaacctg
     3001 tcgtcagccg tcgtctaccg cgcgacgcgc tcgcggcgcc cctggctcaa gaagccgctg
     3061 acgcgctggt tcgcgaaagc ctacggcgtc gacctgaccg aagccgccaa tgccgatctc
     3121 gagagctacg cgacgttcaa cgacttcttc acgcgcgagc tgaagcctaa cgcccggccg
     3181 ctcgagggcg gacccggcac ggtgacctcg ccggccgacg gcgtgctgac cgagcacggc
     3241 gcgatcgaag gcgggcgtct ctatcaagcc aaaggaagcg cgtacacgct cgcggagctg
     3301 ctcggtgaaa gcggcgccgc cgtcgcggcg ctcgaaggcg ggcgctactt cacgatttat
     3361 ctcgcgccgc acaactatca ccgcgtgcat tcgccgctcg acgcgcagct cacgctcacg
     3421 cgctacatcc cgggctcgcg gtacagcgtg agccgcgcga cggttgccgc gatcccgcgc
     3481 ctcttctgcc gcaacgagcg cgtcgtcctg aggttcgaga cgagcttcgg gccgatgatc
     3541 gtcgtgctcg tcggcgcgct gaacgtgtcg agcatcagca cgttcgcgca cggcgagatc
     3601 gcaagcggcg ccgcgcggca gtgggtcgag cagccgccgc tgccggttgc gcgcggggcc
     3661 gagatcgggc gcttcaacat ggggtcgacc gtcgtcgtgc tgctcggctc cgatcgattt
     3721 cggttttcgc cggacctgtc cgacggcctc gccgtgcgca tgggacaagc gctcggctcg
     3781 ttcgcttagg cacgagccgt gagctacgag cgcggcgcat cgctcgagac gctgcggcaa
     3841 cgcgccgcga tgctggccgc cgtgcgcgcg ttcttcgccg cgcgcggcgt gctcgaggtc
     3901 gagacgcccg cgttgtcgag cgcgggcgcg acggatcccg cgctcgaaag catcgcggcg
     3961 aacgcgcgca gcttgggctc gcggcagcag tacttgcaga cctcgcccga gttcgcgatg
     4021 aagcgcctgc tcgccgcggg cagcggcgac atctatcagc tgtgccgcgt gtttcgcgac
     4081 gacgagctcg gccgctggca tcagcccgag ttcatgctgc tcgagtggta ccgcgtgggc
     4141 ttcgacgagc agcggctcat gagcgaagtc gccgagctca tcgcggcggc gctggctgcc
     4201 gcggcgcccg gcggcgcgag cgccgcgccg cgcatcgtgc ggctcacgta cgcgcaggcg
     4261 ctcgaggccg cgctcgggct actgcccgac gcgccgaccg cggagctcgt gcgcgcgctc
     4321 gcgaaacaag gcatcgacgt gccggcgaat ttgagccacg acgccgtgct cgatctcgcg
     4381 ttcggcaccg cagtgctcgc gagcttcgac ggaactgcgg tgacgttcgt ttacgactat
     4441 ccggcaagcc aggcg
//
LOCUS       DASSNN010000012         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1011500, whole genome shotgun sequence.
ACCESSION   DASSNN010000012 DASSNN010000000
VERSION     DASSNN010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011500"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFL84_00205"
     CDS             <1..588
                     /locus_tag="VFL84_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7607068.1"
                     /translation="LVFVGRNDGRLTALDSDTGLMLWEFQTGAGMNAPATTFERDGKQ
                     YVDALSAGNLLIGSAKGDSVWLFGLDGTLPPAAERDTENRTTAVPPPAPAPVAAAATA
                     PAAAAAPGSASAPDALVSAGHEVFATACVPCHGEDGLGGHGGGAPLDQVKDPSLVVAM
                     INDGRGSMPPLAGLLSPEQIRAVAAYVTSPGGLFE"
     gene            600..1619
                     /locus_tag="VFL84_00210"
     CDS             600..1619
                     /locus_tag="VFL84_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009022223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7607069.1"
                     /translation="MIIDCHGHYTTAPKELGDYRDQQKADLKKNPQHQAAKGTLKISD
                     DQLRASVEGAQLKLQRERGTDVTIFSPRASWMGHHLGNASTSKAWTEHCNDLIYRLTK
                     LYPKNFVGVAQLPQSPGAPIDSSVTELTRCVEQLGFVGCNLNPDTSGGYFNGPSLADK
                     YWYPLYEKMCELEVPAMIHVSEACNTPYFQTTGSYYLAADTVAFMQMMTSDLFKDFPD
                     LKLIIPHGGGAVPYHWGRFRGLADMLKRPPLKESVLNNVYFDTCVYHQPGIDLLLDVI
                     PIDNILFASEMVGAVRGIDPETGHYFDDTKRYIDGNKKLSAADRAKIFEGNARRVFPR
                     FPASL"
     gene            1734..>2136
                     /locus_tag="VFL84_00215"
     CDS             1734..>2136
                     /locus_tag="VFL84_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018619118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HET7607070.1"
                     /translation="MNRLLARACIAVTALVLCFSAAAQVLSAKVTGGELQGVASNGVA
                     SFKGVPFAAPPVGDLRWKKPQPAKTWQGVRAANAFGPSCMQDPAMLQVQEAPPATAED
                     CLYLNVWTPAKSASERLPVMVWIYGGGFAIGT"
BASE COUNT          381 a          716 c          693 g          346 t
ORIGIN      
        1 ctcgtgttcg tcggccgcaa cgacggccgc ctgacggcgc tcgactccga taccgggctc
       61 atgctctggg agtttcagac cggagccggc atgaacgcac cggcgacgac gttcgaacgc
      121 gacggcaagc agtacgtcga cgcgctgtcg gccggcaatt tgctgatcgg ctccgcgaaa
      181 ggcgacagcg tgtggctgtt cggcctcgac ggcacgctgc cgccggcggc cgagcgcgac
      241 accgagaacc gcacgaccgc cgtgccgccg ccggcaccgg cgcccgtcgc ggcggctgcg
      301 actgcacccg ctgccgcagc ggcgccgggc tccgcctccg cgcccgacgc gctcgttagc
      361 gcaggccacg aggtattcgc gacggcttgc gtcccgtgtc acggcgagga cggcctcggc
      421 ggccacggcg gcggcgcgcc gctcgatcag gtcaaagatc cgagcttggt cgtggcgatg
      481 atcaacgacg gacgcggcag catgccgccg ctcgcgggcc tgctgtcgcc ggagcagatt
      541 cgcgcggtcg ccgcgtacgt cacgagcccc ggcggtttgt tcgagtagcc aatagaccaa
      601 tgatcatcga ttgccacggc cactacacga cggcgccgaa agagctcggc gactatcgcg
      661 atcagcagaa agccgatctg aagaagaatc cgcagcatca agcggcgaaa ggcacgctca
      721 agatcagcga cgatcagctg cgcgcgagcg tcgaaggcgc gcagctcaag ctgcaacgcg
      781 agcgcggcac cgacgtcacg atcttcagcc cgcgcgcgag ctggatgggg catcacctcg
      841 gcaacgcgtc gacgagcaag gcgtggaccg agcactgcaa cgatctgatc taccggctca
      901 cgaagctgta cccgaagaac ttcgtcggcg tcgcgcagtt gccgcagtcg ccgggcgcgc
      961 cgatcgattc gtcggtgacg gagcttacgc gctgcgtcga gcagctcggc ttcgtcggct
     1021 gcaacttgaa tccggacacg agcggcggct acttcaacgg cccgagcctc gcggacaagt
     1081 actggtatcc gctctacgag aagatgtgcg agcttgaagt gcccgccatg atccacgtca
     1141 gcgaggcgtg caacacgccg tacttccaaa cgacgggctc gtactacctc gccgccgaca
     1201 cggtcgcgtt catgcagatg atgacgagcg atctgttcaa ggactttccg gacctcaagc
     1261 tcatcatccc gcacggcggc ggcgccgtgc cgtatcactg ggggcgcttc cgaggcttgg
     1321 ccgacatgtt gaagcggccg cctttgaagg agtcggtgct gaacaacgtc tacttcgaca
     1381 cctgcgtcta ccatcagccc ggcatcgact tgctgctcga cgtgattccc atcgacaaca
     1441 ttctgttcgc gtcggagatg gtcggcgccg tgcgcggcat cgacccggaa accgggcact
     1501 acttcgacga cacgaagcgc tacatcgacg gcaacaagaa attgagcgcg gccgatcgcg
     1561 ccaagatctt cgaaggcaac gctcgccgcg tgttcccgcg ctttcccgcg tcgctctagc
     1621 catcgccact tcgctctttt cgcgatggcg cggatcgtcg accgaccggg cattgtcgcg
     1681 acgcgtgccc gggcgaataa tggatccccg caatccactt cgggggatca ccgatgaacc
     1741 gcctgctcgc gcgtgcgtgc attgccgtca cggcgctcgt tctttgcttc tcggcggccg
     1801 ctcaagtcct ttccgcgaag gtcaccggcg gcgagctgca aggcgtagca tcgaacggtg
     1861 tcgcgtcgtt caagggagtg ccgttcgcgg cgccgcccgt cggcgatctg cgctggaaga
     1921 agccgcagcc cgcgaagact tggcaaggcg tgcgcgcggc gaacgcgttc ggcccgagct
     1981 gcatgcaaga ccccgcgatg ctgcaagttc aggaggcgcc gcccgctaca gctgaagact
     2041 gcctctattt gaacgtgtgg acgcccgcaa agagcgcaag cgagcggctg cccgtgatgg
     2101 tgtggattta cggcggcggg ttcgcgatcg gcacgt
//
LOCUS       DASSNN010000013         3145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1516268, whole genome shotgun sequence.
ACCESSION   DASSNN010000013 DASSNN010000000
VERSION     DASSNN010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516268"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFL84_00220"
     CDS             <1..465
                     /locus_tag="VFL84_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7607071.1"
                     /translation="IVREDLLGKARRETPGVINYKTMAENDSMWNTPSTLSWYIAGLV
                     FDWLKEQGGLEAMGKVNERKAAKLYRAIDGSGFYKNPVDKAYRSWMNIPFTLPSPELD
                     KPFLQEAKAAGLTNLKGHRVVGGMRASLYNAMPEAGVDALIAFMADFAKRHG"
     gene            476..1654
                     /locus_tag="VFL84_00225"
     CDS             476..1654
                     /locus_tag="VFL84_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET7607072.1"
                     /translation="MFKVRTLNSIAVAGLDRLPRDRYEVASEIARPDAILLRSFKMHD
                     MPLPESLLAVGRAGAGTNNIPVAKLSKIGVPVFNTPGANANAVKELVIAGLLMAARNL
                     PQALDFVRTLKGSGEELDKAVEAGKKAFVGFELPKRTLGVVGLGAIGVEVANAALRLG
                     MNVLGYDPAITVQHAWALSSGVQKAVNLDDLFARADVVTVHVPLTKQTDKLVNAPRLE
                     AMRRGGVILNFSRDAIVDSEAVVAALNAGKLRAYVTDFPTRALLDHPKAICLPHLGAS
                     TVEAEENCAVMVVDNVREFLENGNVTRSVNFPETVLSRTRPHRVAIPHKNVPNMVAQI
                     LTCLAAQNINIADMLNHSRDELSYTLVDLDTQPTEETLERIRGIDGILSVRVLPIIDA
                     "
     gene            1671..2876
                     /gene="pheA"
                     /locus_tag="VFL84_00230"
     CDS             1671..2876
                     /gene="pheA"
                     /locus_tag="VFL84_00230"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET7607073.1"
                     /translation="MKGKPKSKAKLKSRGAAAASVERGRKNASARAALPQSTAVADDE
                     LAALRGRIDAVDERIQALIAERAGLAKEVGILKGLKQTVDYYRPEREAQVLRKVVDRN
                     DGPLRDEEMVRLFRELMSACLAQEEPLKVAYLGPEGTFTQSAVFKHFGHSVHALAVPT
                     VDEVFHEVESGTADFGVAPIENSSEGTVNIHTLDMFLTSPLKICGEIELRIHQHLMGR
                     MQDLKDIKRVCSHPQSLAQCRGWLSQHLPDVEKVPVVSNAEAARRARDEEGTAALAGE
                     AAANVYNLRILFNTVEDRDDNTTRFVVIGRKLFPASGQDKTSVLVSAAETETPGVLLH
                     LLAPLSKHGVNMTRIESRPSRKRKWDYVFFLDLDGHADDEHVRKALDAMKAQASLFKV
                     LGSFPKAVL"
     gene            2917..>3145
                     /locus_tag="VFL84_00235"
     CDS             2917..>3145
                     /locus_tag="VFL84_00235"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019571279.1"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET7607074.1"
                     /translation="MPTNRIGGRITVPGDKSISHRALMLGAIAEGTTVVRGFLASEDC
                     LATQAALEAMGVSIERQPGGVVRIAGVGRHGL"
BASE COUNT          554 a         1040 c         1059 g          492 t
ORIGIN      
        1 atcgtgcgtg aggacctgct cggcaaggcc cgccgcgaga cgcccggcgt catcaactac
       61 aagacgatgg ccgagaacga ttcgatgtgg aacacgccgt cgacgctgtc gtggtacatc
      121 gcggggctcg tcttcgactg gctcaaggag caaggcggcc tcgaggcgat gggcaaggtc
      181 aacgagcgca aggccgcgaa gctctatcgc gcgatcgacg gctcgggctt ctacaagaac
      241 cccgtcgaca aggcgtatcg ctcgtggatg aacattccgt tcacgctgcc gagcccggag
      301 ctcgacaagc cgttcctgca agaggcgaag gccgcgggtc tcacgaactt gaagggccat
      361 cgcgtcgtcg gcggcatgcg cgcgagcctc tacaacgcga tgccggaagc cggcgtcgac
      421 gcgttgatcg cattcatggc cgacttcgcg aagcggcacg ggtgagcgcg tgagcatttt
      481 caaagttcgc acactgaaca gcatcgcggt cgcggggctc gaccggctgc cgcgcgatcg
      541 ctacgaagtc gcgtccgaga tcgcgcgtcc cgacgcgatt ctgctgcgct cgttcaagat
      601 gcacgacatg ccgctgccgg agtcgctgct cgcggtcggc cgcgccggcg cgggcacgaa
      661 caacattccg gtcgcgaagc tgagcaagat cggcgtgccg gtcttcaaca cgccgggagc
      721 taacgcgaat gccgtcaagg agctcgtcat cgcgggcttg ctgatggccg cgcgcaatct
      781 gccgcaggcg ctcgacttcg tccgcacgct caaggggtcc ggcgaggagc tcgacaaggc
      841 ggtcgaggcg ggcaagaagg cgttcgtcgg cttcgagctg ccgaagcgca cgctcggcgt
      901 cgtcggtctc ggcgcgatcg gcgtcgaggt cgcgaatgcc gcgctgcggc tcggcatgaa
      961 cgtgctcggc tacgatcccg cgatcaccgt gcagcatgct tgggcgctgt cgtcgggcgt
     1021 gcagaaggcc gtcaacctcg acgatctgtt cgcgcgcgcg gacgtcgtca cggtgcacgt
     1081 gccgctcacg aagcagacgg acaagctcgt gaacgcgcct cgcctggagg ccatgcgccg
     1141 cggcggcgtg atactgaatt tctcgcgcga cgccatcgtc gattccgagg ccgtcgtcgc
     1201 cgcgttgaac gcgggcaagc tgcgcgcgta cgtcacggac ttcccgacgc gcgcgctgct
     1261 cgatcatccg aaggcgatct gcctaccgca cctcggcgcg tcgacagtcg aagccgaaga
     1321 gaactgcgcc gtcatggtcg tcgacaacgt gcgcgagttc ctcgagaacg gcaacgtcac
     1381 gcgctccgtc aacttccccg agaccgtgct gagccgcacg cggccgcacc gagtcgcgat
     1441 tccgcacaag aacgtgccga acatggtcgc gcagatcctg acgtgcttgg cggcgcagaa
     1501 catcaacatc gccgacatgc tgaatcattc gcgcgacgag ctttcgtaca cgctcgtcga
     1561 tctcgacacg cagccgaccg aggagacgct cgagcggatt cgcggcatcg acggcatcct
     1621 gtctgtgcgc gtgctgccca tcatcgacgc gtgaacgcgc gggagcgctc atgaagggga
     1681 aacccaagtc gaaggctaaa ctcaagtcgc gcggtgccgc ggccgcgagc gtcgagcgcg
     1741 gccgcaagaa tgcttctgcg cgtgcggcgc ttccgcaatc cacggcggtc gccgacgacg
     1801 aactcgcggc gctgcgcggc aggatcgacg ccgtcgacga gcggatccaa gcgctgatcg
     1861 ccgagcgcgc gggcctcgcc aaagaggtcg gcattctgaa agggctcaag cagaccgtcg
     1921 actactaccg cccggagcgc gaggcgcagg tgctgcgcaa ggtcgtcgat cgcaacgacg
     1981 ggccgctgcg cgacgaggag atggtgcgtc tatttcgcga gctgatgtcg gcctgcctcg
     2041 cgcaggagga gccgctcaag gtcgcgtatc tcgggccgga aggcacgttc acgcagtcgg
     2101 ccgtcttcaa gcacttcggc cactcggtgc acgcgctcgc ggtgccgacc gtcgacgagg
     2161 tgttccacga ggtcgagagc ggcacggcgg acttcggcgt cgcgccgatc gagaactcga
     2221 gcgagggcac cgtcaacatc cacacgctcg acatgtttct cacgtcgccg ctcaagatct
     2281 gcggcgagat cgagctgcgc atccatcagc atctgatggg gcgcatgcaa gacctgaagg
     2341 acatcaagcg cgtctgctcg cacccgcagt cgctcgcgca atgccggggc tggctttcgc
     2401 agcatttgcc ggacgtcgag aaagtgcccg tggtcagcaa cgcggaagcg gcgcggcgcg
     2461 cgcgcgacga ggagggcacg gcggcgctgg ccggcgaggc cgccgccaac gtctacaact
     2521 tgcgcatcct gttcaacacc gtggaggacc gcgacgacaa cacgacgcgc ttcgtcgtga
     2581 ttgggcgcaa gctgttcccg gcgagcggcc aggacaagac gtcggtgctc gtgtcggccg
     2641 ccgagaccga gactccgggc gtgctgctgc acttgctcgc gccgctgtcg aagcacggcg
     2701 tcaacatgac gcgcatcgag tcgcggccct cgcgcaagcg caagtgggac tacgtgttct
     2761 ttctcgatct cgacggtcac gccgacgacg agcacgtccg caaggcgctc gacgcgatga
     2821 aggcgcaggc ctcgctgttc aaagtcctgg gctccttccc caaagccgtg ctttgattcg
     2881 agctccgtgt cgcggtaccc caatcgcttt cgcgccatgc cgacgaatcg catcggcggg
     2941 cgcatcacgg tccccggcga caagtcgatc tcgcaccgcg cgctgatgct cggcgcgatc
     3001 gcggaaggaa cgaccgtcgt tcgcggcttt ctcgcgagcg aggattgcct cgcgacgcag
     3061 gccgcgctcg aggcgatggg cgtatcgatc gaacggcagc cgggcggcgt cgtccgcatc
     3121 gccggcgtcg ggcggcacgg ccttg
//
LOCUS       DASSNN010000014         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_381838, whole genome shotgun sequence.
ACCESSION   DASSNN010000014 DASSNN010000000
VERSION     DASSNN010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381838"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VFL84_00240"
     CDS             <1..1321
                     /locus_tag="VFL84_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HET7607075.1"
                     /translation="TRPIERRLWPRDKSSAARTPYWLLFARFAYAMLRDFLHGDLTLR
                     AMSLVYTTMLAIVPLLAAAFSAMKGLGIHRDLEPVLRGFLAPLGPRSGEISASIIGFV
                     DNVSGSTLAITGIVLLLYTAFSMAQKVESSFNFVWRVDRPRSFARRFGEYLSFMLLGP
                     FIMSVAMGFTATLASAAAMSRLHEIGVIGRMLDALSWFTPYAVIIASFTVLYLIIPNT
                     RVRFKPALIGGVFAGILWAGGGSLFTSFIVSVSRYEAIYAGFAIVLVAMIWLYLSWVI
                     LLLGAQLAFYVQHPEYLPLGQRAPSASNATRERLALSVMLSVGRDFDKPGHGWRIESL
                     AASLRVQRHQLEPIVTALIDEGLLTRTRENRLIPARDLRRIGVAEIFAAVRSSEHDSH
                     HEFDDEWNATVARVAGEVERAIRGTLGERTLADLVDADKKGTDLFSG"
     gene            complement(1540..>2216)
                     /locus_tag="VFL84_00245"
     CDS             complement(1540..>2216)
                     /locus_tag="VFL84_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET7607076.1"
                     /translation="RRALQRLNARTLSTRKVPVLYPAELAKGLFGHLIAAVRGTAQYR
                     RASFLLDAVGKQVLPSFIDIDEDPWIPRALASAPFDAEGVATRQRRLVSRGVLEGYVL
                     SSYSARRLGLVTTGNAGGIHNLLVKPTAGPLEALIKDCNEAFVVGELLGQGVNTVTGD
                     YSRGAAGFWVERGEIVHPVHEVTIAGNLKDLFREIQAVGSDVDPRGTVRCGAVRVDGL
                     TLAGAG"
BASE COUNT          363 a          739 c          721 g          393 t
ORIGIN      
        1 cacccggccg atcgagcgcc gcctctggcc gagggacaag agcagcgcgg cgcggacgcc
       61 gtactggctc ctcttcgcgc gctttgcgta cgcgatgctg cgcgatttct tgcacggcga
      121 cctgacgctg cgcgcgatga gcctcgtcta cacgacgatg ctcgcgatcg tgccgctgct
      181 cgccgcggcg ttctcggcca tgaagggctt gggcatccac cgcgatctcg agccggtcct
      241 gcgcggtttc ctcgcgccgc tcggcccgcg ctcgggcgag atctcggcga gcatcatcgg
      301 cttcgtcgac aacgtcagcg gctcgacgct cgcgatcacg ggcatcgtgc tgctgctgta
      361 caccgcgttt tcgatggcgc aaaaagtcga gagcagcttc aacttcgtct ggcgcgtcga
      421 ccggccgagg agctttgcgc gccgcttcgg cgaatacctg agcttcatgc tgctcggccc
      481 gttcatcatg agcgtagcga tgggcttcac ggcgacgctg gcgagcgcgg ccgcgatgag
      541 ccggctgcac gagatcgggg tcatcggccg aatgctcgac gcgctgtcgt ggttcacgcc
      601 gtacgccgtc atcatcgcga gcttcacggt cttgtacctg atcattccga acacgcgcgt
      661 gcgcttcaaa ccggcgctga tcggcggcgt gttcgccggc atcctctggg ccggcggcgg
      721 cagcctgttc acgagcttca tcgtgtcggt gagccgctac gaagcgatct acgcggggtt
      781 cgcgatcgtg ctcgtcgcaa tgatttggct gtatctgtcg tgggtgattc tgctgctcgg
      841 agcgcagctc gcgttctacg tccagcaccc cgagtacttg ccgctcggcc agcgggcgcc
      901 gagcgcgtcg aacgcgacgc gcgagcggct cgcgctgtcc gtcatgcttt ccgtcggccg
      961 cgacttcgac aagcccggcc acggctggcg catcgaaagc cttgccgcga gcctgcgcgt
     1021 gcagcggcac cagctcgagc cgatcgtcac ggcgctgatc gacgaagggc tgctcacgcg
     1081 cacgcgcgag aaccgcctga tacctgcgcg cgacctgcgc cgcatcggcg tcgccgagat
     1141 cttcgcggcc gtgcgcagca gcgagcacga ctcgcaccac gagttcgacg acgagtggaa
     1201 cgcgaccgtc gcgcgcgtcg cgggcgaggt cgagcgcgcg atccgcggca cgctcggcga
     1261 gcgcacgctg gccgatctcg tcgacgccga caaaaagggg acagatctat tttccggatg
     1321 aaaaggggac cggatgaaaa ggggacagat ctattttcgg tcgaaaatga aatttatccc
     1381 cctaaagcct ggagctaaaa cagatctgtc cccttttcag ctctaaatag atctgtcccc
     1441 ttttcgcgct tttgctagcc gaaaaataga tctgtcccct tttcgaaaat agatctgtcc
     1501 ccttttctag ccgaaaatag atctgtcccc ttttcggggc tagccggcgc cggcgagggt
     1561 gaggccgtcg acgcgcacgg cgccgcagcg caccgtgccg cgcgggtcga cgtcggagcc
     1621 gaccgcctgg atctcgcgga acaggtcctt gaggttgccg gcgatcgtga cctcgtgaac
     1681 cggatggacg atttcgccgc gctcgaccca gaaaccggcg gcgccgcgcg agtagtcgcc
     1741 cgtgacggtg ttgacgcctt ggccgagcag ctcgccgacg acgaacgctt cgttgcaatc
     1801 cttgatcagc gcctcgagcg ggccggccgt cggcttcacg agcaggttgt gaatgccgcc
     1861 ggcattgccc gtcgtgacga ggccgagccg ccgcgccgag tagctcgaca gcacgtagcc
     1921 ttcgagcacg ccgcgcgaga cgaggcggcg ctggcgcgtc gcgacgccct cggcatcgaa
     1981 cggtgcgctc gcaagcgcac gcggaatcca cggatcttcg tcgatgtcga tgaaggacgg
     2041 caggacctgc ttgccgaccg catcgagcag gaacgacgct cgccggtatt gcgccgtgcc
     2101 gcgcacggcc gcaatcaaat ggccaaacag ccctttggcg agctcggccg gataaagcac
     2161 gggcaccttg cgcgtcgata gcgtacgcgc gttcaagcgc tgcagcgcgc gccgcg
//
LOCUS       DASSNN010000015         2925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1138692, whole genome shotgun sequence.
ACCESSION   DASSNN010000015 DASSNN010000000
VERSION     DASSNN010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138692"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VFL84_00250"
     CDS             complement(<1..624)
                     /locus_tag="VFL84_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607077.1"
                     /translation="MRNWLVVAALLILPIVVYWPTVTHEYGFRDDYSNLREVRERPGW
                     LMTLTSSSGRPVYGVLLEGSLSQIYQVTELAKLRALSAVLAGVVGVLLWWQLRRSGWG
                     EMQAAAMGAAVILLPGIQVVVGWAIAWPIMLALAFALIAFSLVERGMHTARLVGSVCI
                     AAGAALYFIVGLTYQTSALFGIMPFTAALLLRPETSARNDSRWIIVHV"
     gene            complement(627..1322)
                     /locus_tag="VFL84_00255"
     CDS             complement(627..1322)
                     /locus_tag="VFL84_00255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7607078.1"
                     /translation="MDEAEFDRFADEYRAMHAANIRLSGEDPEYFAEYKIVDIAAELA
                     RNAIVPRRVLDFGAGVGYSVPFFARHLPAARLTCLDVSRKSLDVGVAHHGGAADFEHF
                     DGRSIPFEDGTFDAALASCVFHHIPHDEHVALLGEIRRVLRPGGRLFVFEHNPLNPLT
                     RHAVNTCEFDVHAKLVGAPTMRRRMRDAGFVDAAIRYRIFFPRALRGMRPLEAKMTWL
                     PLGAQYYVVARKS"
     gene            complement(1325..2167)
                     /locus_tag="VFL84_00260"
     CDS             complement(1325..2167)
                     /locus_tag="VFL84_00260"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HET7607079.1"
                     /translation="MSGVKLIVNADDFGIAEAVNRGIVEAHDRGIVTSTSIMATSPQF
                     EHAVELARLRPSLAVGVHLVLTEQRPLTGAAAASLVRGDGTFEPHLKQLLAKRLRGRV
                     SMAEVRRELDAQIRRVRTAGIAVNHLDGHQHVHVLPGVAQVVAELAAAHGISAVRYPA
                     ERLRGYMLRSVKHAPRVAEQAALNLFCAASPLKHLRRSDEFVGFYFGGRLDEANLETV
                     LAGLPRGGTVELMCHPGHEDMRPRADWQYSWAAERDALTSPRIRALVMARGMQLVSHP
                     NIRG"
     gene            complement(2164..2571)
                     /locus_tag="VFL84_00265"
     CDS             complement(2164..2571)
                     /locus_tag="VFL84_00265"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET7607080.1"
                     /translation="MPLFRLRHPFARFAVVGCANFVVSFAAFYASYTYLPLGDSSGRG
                     AVANVLAYCAGLVNSFVLNRLWTFRAEGHVASHALKFGLLNAATLVGSTVVVLLLVDR
                     AGLPALAVWLPLTLAILATHYLGMKHWAFAERR"
     gene            complement(2581..>2925)
                     /locus_tag="VFL84_00270"
     CDS             complement(2581..>2925)
                     /locus_tag="VFL84_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607081.1"
                     /translation="WHGVSSFSLVPLRIGIAVGLLSSTFAFLGTAYAIVGWWVDDAVV
                     PGWASSVAMTSFLFGVLFAFLAVLAEYVGRIVVEVRRRPRFLVRETTRPALASADRRT
                     PRRGPADARAEG"
BASE COUNT          549 a          990 c         1001 g          385 t
ORIGIN      
        1 gacgtgaacg atgatccaac gagagtcgtt gcgcgcgctc gtctcggggc gcagcagcag
       61 cgcggccgtg aacggcatga tcccgaacag cgcgctcgtt tgatacgtga gccctacgat
      121 gaaatagagc gcggcgccgg ccgcgatgca caccgagccc acgagccttg ccgtgtgcat
      181 gccgcgctcg acgagcgaga acgcgatcag cgcgaaggcc agtgcgagca tgatgggcca
      241 cgcgatggcc caaccgacga cgacctggat gccgggaagc agtatgacgg cggcccccat
      301 tgccgcggcc tgcatctcgc cccagccgga acgccggagc tgccaccaga gcagcacgcc
      361 gacgacgccg gcgagcacgg cggagagcgc gcgcagcttc gcgagctccg tgacctggta
      421 gatctggctc agcgagcctt cgagcagcac accgtacacg gggcggccgc tcgacgaggt
      481 cagcgtcatg agccagcccg gccgctcgcg gacctcgcgc aggttcgaat agtcgtcgcg
      541 aaagccgtat tcgtgcgtga cggtgggcca gtagacgacg atcggtagga tcagcagagc
      601 ggcgacgacg agccaattgc gcatggctac gacttccgcg ccacgacgta gtactgcgcg
      661 ccgagcggca gccaggtcat cttcgcctcg agcggacgca tgccgcgcag agcgcgagga
      721 aagaaaatgc ggtaccggat cgcggcgtcg acgaacccgg catcgcgcat gcgccgccgc
      781 atcgtcggag cccccacgag cttggcgtgc acgtcgaact cgcacgtgtt gacggcgtgg
      841 cgcgtcaacg gattcagcgg attgtgctcg aagacgaaca ggcgcccgcc cgggcgaagc
      901 actcggcgga tctcgccgag cagcgcgacg tgctcgtcgt gcggtatgtg atggaacacg
      961 cacgaagcga gcgcggcgtc gaacgtgccg tcctcgaacg gaatgctgcg gccgtcgaag
     1021 tgctcgaaat cggcggcgcc gccatgatgc gcgacgccga cgtcgaggct ttttcgcgag
     1081 acgtcgaggc aggtcagccg cgccgccggt aaatggcgcg cgaagaacgg caccgagtag
     1141 ccgacgccgg cgccgaaatc gagcacgcgc cgcggcacga tcgcgttgcg cgcgagctcg
     1201 gccgcgatgt cgacgatctt gtattcggcg aagtactcgg ggtcctcgcc ggacagccgg
     1261 atgttcgccg cgtgcatcgc gcggtactcg tcggcgaacc tgtcgaactc ggcctcgtcc
     1321 acgctcaacc tcgaatgttc ggatgcgaaa cgagctgcat gccgcgcgcc atgacgagcg
     1381 cgcgaatacg cgggctcgtc agcgcgtcgc gctccgccgc ccacgagtac tgccaatcgg
     1441 ctcgcggccg catgtcctcg tggcccggat gacacatgag ctccaccgtt ccgcccctgg
     1501 gcaagcccgc gagcacggtc tcgaggttgg cctcgtcgag ccgcccgccg aagtagaagc
     1561 ctacgaattc gtcgctgcgc cgcaaatgct tcaagggcga agcggcgcaa aagagattca
     1621 atgccgcctg ctcggcgacg cgcggggcat gcttcacgct gcgcagcatg tagccgcgca
     1681 gccgctcggc gggatagcgc acggccgaga tgccgtgcgc ggccgcgagc tcggcgacga
     1741 cctgcgcgac accgggcagc acgtggacgt gctgatggcc gtcgaggtga ttgaccgcga
     1801 tgccggccgt gcgcacgcgg cggatctgcg cgtctagctc gcgacgaacc tcggccatgg
     1861 atactcgacc gcgcaaccgt ttggccagca actgcttcag gtgcggctcg aacgtgccgt
     1921 cgccgcgaac gaggctcgca gcggccgcgc cggtcagcgg ccgctgctcc gtcagcacga
     1981 ggtgtacacc gacggcgagg ctcgggcgca atcgcgcgag ctcgacggcg tgctcgaatt
     2041 gcgggctcgt ggccatgatc gacgtcgacg tcacgatgcc gcggtcgtgc gcctcgacga
     2101 tcccgcggtt gacggcttcg gcgattccga agtcgtcggc gttgacgatg agcttcacgc
     2161 cgctcatcgc cgctccgcga acgcccaatg cttcatgccg agataatgcg tcgcgaggat
     2221 tgcgagcgtc agcggcagcc agacggcgag cgccggcaat cccgctcgat cgacgagcag
     2281 cagcacgacg acggtgctgc ccacgagcgt cgcggcattg agcagcccga acttcagcgc
     2341 atgcgaagcg acgtgtcctt cggcgcgaaa cgtccagagg cgattcagca cgaagctgtt
     2401 gacgagaccg gcgcaatacg cgagcacgtt cgcgacggcg ccgcggccgc tcgagtcgcc
     2461 gagcggcagg tacgtatacg acgcgtagaa cgccgcgaag ctcacgacga aattggcgca
     2521 cccgacgacg gcgaagcgcg caaagggatg ccgcagacgg aagagcggca cgcgcagcgc
     2581 tcaaccttcc gctcgagcgt ccgcgggccc gcgccgcggc gtccgccgat cggccgaggc
     2641 gagggccggc cgcgtcgtct cgcgcacgag gaaccgcggc ctgcggcgca cttcgacgac
     2701 gattcgcccg acgtattcgg ccaagaccgc gagaaacgcg aacagcacgc cgaacaagaa
     2761 cgaggtcatg gccaccgaag atgcccagcc aggcacgaca gcgtcgtcaa cccaccagcc
     2821 gacgatcgcg taggccgtgc cgaggaacgc gaacgtcgag gacaggagcc cgaccgcgat
     2881 accgattcgc aacggcacga gcgagaacga gctcacaccg tgcca
//
LOCUS       DASSNN010000016         3375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_758654, whole genome shotgun sequence.
ACCESSION   DASSNN010000016 DASSNN010000000
VERSION     DASSNN010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758654"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFL84_00275"
     CDS             <1..280
                     /locus_tag="VFL84_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018935472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7607082.1"
                     /translation="SRFVKDLRETREGAALVAAIVGMAHGLGIRVIAEGVETVDALNM
                     LKELGCDEAQGFLISPPVGAREAFALAGTEFAFRNEPAVERRPSTAVG"
     gene            complement(287..1195)
                     /locus_tag="VFL84_00280"
     CDS             complement(287..1195)
                     /locus_tag="VFL84_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7607083.1"
                     /translation="MTRPGWLAAAALFLAAGCACAQEPPREEAVTTEQIAPDLHVLFG
                     AGGGRVSGNVLALTAAQGTLVVDTGYAVHVPKYRAAIAALHGGPITRVVNTHWHDDHS
                     EGNKLLGPGGALIVAHTNAREMLRHDNKINVVRTILDQAAFPPAALPVVTFDGRSELY
                     FAGERIELVHVAPAHTAGDIVVILREHNVAHTGDVFLPAAYPFSDVDNGGDFDGEIAF
                     VTALLTELNRDTVIVPGHGRVARYDELIAYVDMLKAVRERIAALIAGGATLEQVVAAE
                     PTKEWDAKYGNPRTYFLDRSYKSLSR"
     gene            complement(1263..>3375)
                     /locus_tag="VFL84_00285"
     CDS             complement(1263..>3375)
                     /locus_tag="VFL84_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7607084.1"
                     /translation="ALTTRDERVHMNDMTAMNGWPARALLAATLASLGAATAAAQPPA
                     PGSREDEPQTIVVTASRRAQELKDAPAAMTVIGGAEIERLPADDYGDLLRNVPGLNVA
                     QTSVRDINLTGRGSTSTLANTQLVLLDGRSVYLDFFGFVMWDLLPIQANEIERIEVVR
                     GPGSAVWGANAMAGVVNVIGRRPKDMAGTTVLVGTPYASVVHAAASDGFGYKVSAGYF
                     EQPAYDRPTGTIPGSNPPQTYPDYENEGTEQARVNALLEWETGDDAYVSVAAGYAETD
                     GILHSGIGPFDIEKGSNLSYVKSDWNSGSLHLGASATMLDGDAVNLLTRGADGLPLPL
                     SFVSDAYTLDVSNTSELGPRHVLTYGASYRTTKFDLGIAPAADDRDEIGGFLEDEIRL
                     GEHLRWVLGARYDDIDPLAHAVVTPRTSLIIAPSAVHSFRVSYNEAFRTPSAINGYLD
                     VTILQQLGPFFVPADADGNPLLDEERLEAYEIGYVGTFDNGLVVSAAVYRNDIEDSID
                     FYVRDVYGPANLPTPSATLPPAVVPCFLFAPGMGPAGCPFAGLAGLVPSDYSYRNIGT
                     TLNRGVELSLERAVDEWSWFVNLSWQDEPEIGGGIDAVEINVAPAWRANLGLAHDAGR
                     HFWDVGLNYQDDAYWADVLFARASTPAFTQVNAAFGWRFASDKVTLKLIAQNLFDEDV
                     QQHIFGDIIGRKLAAQVSVEL"
BASE COUNT          516 a         1134 c         1168 g          557 t
ORIGIN      
        1 ctcgcggttc gtcaaagacc tccgcgaaac gagggagggt gcggcgctcg tggccgccat
       61 cgtcggcatg gcgcatggtc ttggcattcg cgtcatcgcg gaaggcgtcg agaccgtcga
      121 tgcgctgaac atgctcaagg agctcggctg cgacgaggcg caaggcttcc tgatctcgcc
      181 gccggtgggt gcgcgcgaag ctttcgcgct cgcagggacg gaatttgcgt ttcgcaacga
      241 acccgccgtc gagcggcgcc cttctaccgc cgtcggctga cggtagtcag cggctgaggc
      301 tcttgtagga gcggtccagg aagtacgtgc gcgggttgcc gtacttcgcg tcccactctt
      361 tggtcggctc ggccgcgacg acctgctcga gcgtggcacc gcccgcgatc agcgcggcga
      421 tgcgctcgcg cacggccttc agcatgtcga cgtaggcgat gagctcgtcg tagcgcgcga
      481 ccctgccgtg ccccggcacg atcacggtgt cgcggttcag ctccgtgagc aacgccgtca
      541 cgaacgcgat ctccccgtcg aagtcgccgc cgttgtcgac gtcggagaac ggataggccg
      601 cgggcaagaa cacgtctccc gtgtgcgcga cgttgtgctc gcgcaggatc acgacgatgt
      661 cgccggccgt gtgcgcgggc gcaacgtgca cgagctcgat gcgctcgccg gcgaagtaga
      721 gctcgctgcg gccgtcgaac gttacgaccg gcagcgcggc cggcggaaac gcggcctgat
      781 cgaggatcgt gcgcacgacg ttgatcttgt tgtcgtgcct cagcatctca cgcgcgttcg
      841 tgtgcgcgac gatcagcgcg cccccggggc cgagcagctt gttgccctcc gagtggtcgt
      901 cgtgccagtg cgtgttgacc acgcgcgtga tcgggccccc atgtagagcc gcgatcgccg
      961 cgcgatactt cggcacgtgc accgcgtagc cggtgtcgac gacgagcgtg ccttgcgccg
     1021 ccgtcagcgc cagcacattg ccggagaccc ggccgccgcc ggcgccgaac agcacgtgca
     1081 agtcgggcgc gatctgctcc gtcgtcacgg cctcttcgcg cggcggctct tgagcgcacg
     1141 cgcagccggc ggccaagaac agcgcggccg ccgcaagcca tcccggtcga gtcattgcag
     1201 atccttcgat cgccgccatc caagacttgt acggggcgcg cggcggccgt gtcaatcggg
     1261 gctcagagct cgacgctgac ttgcgcggcg agcttgcggc cgatgatgtc gccgaagatg
     1321 tgctgctgaa cgtcctcgtc gaacaggttc tgcgcgatga gcttcaacgt gactttgtcc
     1381 gacgcgaagc gccagccgaa cgccgcattg acctgggtga acgcgggcgt ggaggcgcgc
     1441 gcgaacagca cgtcggccca gtaggcgtcg tcctgatagt tcaggccgac gtcccagaag
     1501 tgccggccgg cgtcgtgcgc caagcctaag ttggcgcgcc aagccggcgc tacgttgatc
     1561 tcgacggcgt cgatgccgcc gccgatctcg ggctcgtcct gccacgagag attcacgaac
     1621 cagctccact cgtcgacggc gcgctcgaga gacagctcga cgccgcgatt caaagtcgtg
     1681 ccgatgttgc ggtagctgta gtcggaaggc actaaacctg cgaggcccgc gaacgggcac
     1741 cccgcgggcc ccatgcccgg cgcaaacaag aagcacggca cgacggccgg cggcagcgtc
     1801 gcgctcggcg tgggcaagtt ggccgggccg tagacgtcgc gcacatagaa gtcgatcgag
     1861 tcctcgatgt cgttgcgata gaccgccgcg ctgacgacga gcccgttgtc gaacgttccg
     1921 acgtagccga tctcgtacgc ctcgagccgc tcctcgtcga gcagcgggtt gccgtcggca
     1981 tcggccggca cgaagaacgg cccgagctgc tgcagtatcg tcacgtcgag atagccgttg
     2041 atcgccgagg gcgtgcggaa cgcctcgttg tacgacacgc ggaacgagtg cacggccgac
     2101 ggcgcaatga tcaagctcgt gcgcggcgtg acgacggcgt gcgcgagcgg atcgatgtcg
     2161 tcgtagcgcg cgccgagcac ccagcgcaga tgctcgccca accggatctc gtcttcgagg
     2221 aagccgccga tctcgtcgcg gtcgtcggcc gcgggcgcga tgcccaaatc gaacttcgtc
     2281 gtgcggtagc tcgcgccgta cgtcagcacg tgccggggcc cgagctcgga cgtgttcgag
     2341 acgtcgagcg tatacgcgtc ggagacgaag ctgagcggca gcggcaagcc gtcggcgccg
     2401 cgcgtcagca ggttgacggc gtcgccgtcg agcatcgtgg ccgatgcgcc gaggtggagg
     2461 ctgccgctgt tccaatcgct tttcacgtac gagagattcg agcccttctc gatgtcgaac
     2521 ggcccgatgc cgctgtgcag aatgccgtcc gtctcggcgt atccggccgc gacggaaacg
     2581 tacgcgtcgt cgccggtctc ccattcgagc aaggcgttga cgcgcgcctg ctcggtgcct
     2641 tcgttctcgt aatcggggta ggtttgcggc ggattggagc ccgggatcgt cccggtcgga
     2701 cggtcgtacg ccggctgctc gaagtagccg gccgacacct tgtagccgaa gccgtcgctc
     2761 gcggccgcgt gcacgacgct cgcgtacggc gtgccgacca gcaccgtcgt gccggccatg
     2821 tctttcggcc gccggccgat cacgttgacg acgccggcca tcgcgttcgc gccccagacc
     2881 gcgctgcccg ggccgcgcac gacctcgatg cgctcgatct cgttcgcctg gatcggcagc
     2941 aagtcccaca tcacgaagcc gaagaaatcg agatagacgg aacggccgtc gagcagcacg
     3001 agctgcgtgt tcgcgagcgt cgacgtggag ccgcggccgg tcaggttgat gtcgcgcacg
     3061 ctcgtctgcg cgacgttgag gccgggaacg ttgcgcagca gatcgccgta gtcgtcggcc
     3121 ggcagccgct cgatctccgc gccgccgatc acggtcatcg cggccggcgc gtctttgagc
     3181 tcctgcgctc ggcgcgaggc cgtgacgacg atcgtttgcg gctcgtcctc gcgcgagccg
     3241 ggcgcgggcg gttgcgccgc ggcggtcgcg gcgccgagcg aagccaacgt cgccgcgagc
     3301 aacgctcgcg cgggccatcc gttcatcgcg gtcatgtcgt tcatgtgcac tctctcgtct
     3361 cgagtggtga gcgcc
//
LOCUS       DASSNN010000017         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_382446, whole genome shotgun sequence.
ACCESSION   DASSNN010000017 DASSNN010000000
VERSION     DASSNN010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382446"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFL84_00290"
     CDS             complement(<1..450)
                     /locus_tag="VFL84_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7607085.1"
                     /translation="MFERRIAAAAALFVWLACAAAQEGPKLGVPATPEQIAGWDVSIG
                     PDGAGLPPGSGTAAAGKTVYEAKCLACHGVDGKGQPNDQLVGGQGTLREAAPVRTIGS
                     YWPYATTVFDYIRRAMPYVAPHSLTADETYALTAYLLALNGVIDKDAV"
     gene            complement(443..1711)
                     /gene="soxC"
                     /locus_tag="VFL84_00295"
     CDS             complement(443..1711)
                     /gene="soxC"
                     /locus_tag="VFL84_00295"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HET7607086.1"
                     /translation="MTKREGLDSLEQVAGNGLLHRRLFLQGGAAALAGATLLTARAAP
                     PDLPTWMKAPGAPMRGYGERSPHEAKVQRTVNAAPGTTGTGGSRTPLEALEGIITPSS
                     LHFERHHSGVPDIDPAQHQVLIHGLVERPLIFSMEALARYPMVSRIQFLECSGNSAAN
                     NNAEAPQQTAGGLHGLVSCSDWAGVPLAVLLDEAGVKPEGKWLLAEGADAASMSRSVP
                     MAKAMDDAILALYQNGERLRPEQGYPLRLFLPGYEGNMNVKWLRRIKVTAEPTMTKDE
                     TSKYSDLQPNGRAKLFTFPMEVKSVITSPSGMQTMQGPGLYQISGVAWSGFGRVRRVE
                     ISADGGKSFAPAALDGPVLPQALTRFRMAWRWDGSPSVLMSRAVDETGAVQPTRSALL
                     AARGAQFRYHYNAIQSWNVAPGGAVSNVYV"
     gene            complement(1729..2169)
                     /locus_tag="VFL84_00300"
     CDS             complement(1729..2169)
                     /locus_tag="VFL84_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7607087.1"
                     /translation="MHQYRWPSLLGAIAVCSIAFAAEDEKGFKWLAPSELVYQPNASG
                     PDIAIVAGDPTKEGFYIIRARFKPGVMSRPHYHPTARHVTVISGTWWAGRGATFDPNS
                     TIPLGPGSYMLHPAGEIHYDGAKDVEAIVEIKGIGPAPTIPAAQ"
BASE COUNT          325 a          777 c          743 g          380 t
ORIGIN      
        1 gacggcgtct ttgtcgatga cgccgttcaa cgcgagtaag tacgcggtca gcgcataggt
       61 ttcgtcggcc gtcagcgaat gcggcgcgac gtacggcatc gcgcggcgga tatagtcgaa
      121 caccgtcgtc gcgtacggcc agtagctgcc gatcgtgcgg accggcgccg cttcgcgcag
      181 cgtgccctgc ccgccgacga gctgatcgtt cggctggccc ttgccgtcga cgccgtgaca
      241 cgcgaggcac ttcgcttcgt agacggtctt gccggcggcc gccgtgccgg agcccggcgg
      301 caagccggcg ccgtcggggc cgatgctgac gtcccagcca gcgatctgct cgggcgtcgc
      361 cggcacgccg agcttgggcc cttcttgcgc cgccgcgcat gcgagccaaa cgaacaatgc
      421 ggcagccgcc gcgatgcgcc gctcaaacat agacgttgct cactgcgccg cccggcgcga
      481 cgttccagct ctggatcgcg ttgtagtggt agcggaactg tgcgccgcgc gcggccaaca
      541 gcgcggagcg cgtcggctgc acggcgcccg tttcgtcgac ggcgcggctc atcagcacgc
      601 ttgggctccc gtcccagcgc cacgccatgc ggaagcgcgt cagcgcttgc ggcagcaccg
      661 ggccgtcgag cgcggcgggc gcgaagctct tgccgccgtc cgccgaaatc tcgacgcggc
      721 gcacgcggcc aaagccggac cacgccacac ccgagatctg atacagcccc ggaccttgca
      781 tcgtctgcat gccggacggc gaagtgatga cggacttcac ctccatcggg aacgtgaaca
      841 gcttcgcgcg gccgttcggc tgaaggtccg agtacttcga cgtctcgtcc ttcgtcatcg
      901 tcggctcggc cgtcaccttg atgcggcgca gccacttcac gttcatgttg ccttcgtagc
      961 ccggcaggaa cagccgcaag ggatagccct gctcgggccg cagccgctcg ccgttctgat
     1021 agagcgcgag gatcgcgtcg tccatcgcct tcgccatcgg cacgctgcgg ctcatcgacg
     1081 cggcgtcggc gccttcggcg agcagccatt taccttccgg tttcacaccg gcttcgtcga
     1141 gcaacaccgc gagcggcacg ccggcccagt cgctgcacga gacgaggccg tgcaggccgc
     1201 cggccgtctg ctgcggcgct tcggcgttgt tgttcgcggc gctattcccc gagcactcga
     1261 ggaactggat gcgcgagacc atcggataac gcgcgagcgc ttccatgctg aagatgagcg
     1321 gccgctcgac gagaccgtgg atcaacacct gatgctgcgc gggatcgatg tcgggcacgc
     1381 cgctgtggtg ccgctcgaag tgcaacgagc tcggcgtgat gatgccttcg agcgcctcga
     1441 gcggcgtgcg cgagccgccc gtgcccgtcg tgcccggtgc cgcgttgacc gtgcgctgca
     1501 ctttcgcctc gtgcggcgag cgctcgccgt agccgcgcat cggcgcgccg ggcgctttca
     1561 tccatgtggg caaatccggc ggcgcggcgc gcgccgtcaa aagcgtcgcg cctgcgagcg
     1621 cggcggcgcc gccttgcagg aacaaccgcc gatgcagcaa gccgttgccg gccacttgct
     1681 cgagcgagtc gagcccttct cgtttcgtca tcgcatgagc ctcgccggct actgcgcggc
     1741 cggaatcgtc ggcgccgggc cgatgccttt gatctcgacg atcgcctcga cgtctttcgc
     1801 gccgtcgtaa tggatctcgc cggccggatg cagcatgtag ctgccggggc caagcggaat
     1861 cgtgctgttc ggatcgaagg tcgcgcctct gcctgcccac cacgtgcccg agatcacggt
     1921 gacgtgccgg gccgtcggat ggtaatgcgg ccgcgacatc acgcccggct tgaatcgcgc
     1981 gcggatgatg tagaagccct ctttcgtggg atcaccggcg acgatcgcga tgtcgggccc
     2041 cgaagcgttc ggctggtaca cgagctcgct cggcgcgagc catttgaagc ccttctcgtc
     2101 ctctgccgcg aacgcgatgc tgcagacggc gatcgcgccg agcagcgatg gccaacgata
     2161 ctggtgcatt tggttccccc gtcgcgcgct gcatggcgcg tgcaatgttt cggccaacgc
     2221 tacta
//
LOCUS       DASSNN010000018         4818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1012914, whole genome shotgun sequence.
ACCESSION   DASSNN010000018 DASSNN010000000
VERSION     DASSNN010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012914"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VFL84_00305"
     CDS             complement(<1..611)
                     /locus_tag="VFL84_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607088.1"
                     /translation="MRLFERRHAAARRMVAPLACCALAACGSGDGFVGSGGVVGPLEP
                     SFNSIQANVFDQYCEHCHSGANAPVGLRLDAANSYASLVGVPSGERSGVLRVAPGDAN
                     NSYLIQKLEGTAAVGERMPAGLPPLPQSDINVIRQWIGDGALPAAAGLGPIRVTSVTP
                     EPSSKMLALPASIVVGFDRELNAPSVTTATFTLTRAGADGLLGT"
     gene            complement(728..2434)
                     /locus_tag="VFL84_00310"
     CDS             complement(728..2434)
                     /locus_tag="VFL84_00310"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET7607089.1"
                     /translation="MRLSLFVRASTLAAVASASFLAQAVVAQQAALSGRVTSSAEGAM
                     EGVLVSAKREGSNKTVTVVSHADGRYSFPSDRLEPGRYAVTVRAVRYVLPKHAASVDV
                     AAGKPARLDLELTSANPLELALQLTDPEWLASWPIEDRTKWELFRDCSRCHTLRRPAM
                     STYDAEELSWVLMRRVYSAGSSPMRFQLPANLVPHWGRTEGGEPSALQKRQAEAVASI
                     NLRSGMWPYELKTMPRPKGKETEVVYTTWDLPATARPHDTRIAADGMIWFNHFNDNAI
                     GRLDPKTGEVKEWHWPYRAKEGSFQPTGARTLMGPDARGRWYIGNQAQSGVVVFDPKT
                     EQFEYHDPPGGGEMIDVSGARVDGKAWRSVAAPVAGGAVYQIDLDTWQHVEIKGTNEK
                     PLYAYDIAADSKNNVYGSARNAPYVWRVDAKTHAVQYFDIPVEPRGAGSFGTGMRRGI
                     TDKQDRLWWGGFDGGFVGMLDPRKPKGEEMKLYAVPFPYFFPYDAYYDEQGYTWTAGI
                     YADRVARMNVETGEWNFYLLPFEANIRDINLKPAERNGLSGLWLGHTHQAMITLVEPL
                     AR"
     gene            complement(2553..3284)
                     /locus_tag="VFL84_00315"
     CDS             complement(2553..3284)
                     /locus_tag="VFL84_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607090.1"
                     /translation="MRTLLGIGALTAAALASGTASAAQVVVGESRIAIDVEFWGRQRL
                     VNPDPRDPTNDVITFGDPVHGTFRISANDAPLPRATGGVSFDGKIYGQVTGGNPPSGA
                     SFVTSRWLSPFPKGPVGDITHQVSPFPGSVADDYVIVGDDVRLRAPDLLHADWFEVSD
                     RFGPSSADDRLSGEQLTIGIRSPLDFIDGFGLNQAFELDDLQEKDHTSAFGFFRAKLD
                     TSATAVAFFDFALDRIRVTPRVCKP"
     gene            complement(3411..>4818)
                     /locus_tag="VFL84_00320"
     CDS             complement(3411..>4818)
                     /locus_tag="VFL84_00320"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HET7607091.1"
                     /translation="VPHGRDSALVVVLDVGDPANAYSRMLDLAYTDRATTLPTREYFL
                     EELARFTDIARLKELRAAVICLSIEQSEGHANAFSGQSQDLVLKELASRLVLSLRGVN
                     SHSELDYERYSVAARIDYRQYGVLLPSIQSGADAESVALRLVEALELPVEVGDRQVAV
                     TARAGIALFPQDGADVDTLFQNALTAMEDARSSQTDSYKFHSGTSKLRALQRNELELE
                     LKTALEREQFHLEYLPIVNARSGSVAAVEALLRWPQAAFGTQRTQKVVALAEHTGLIV
                     PIGEWVMQRSFSDLRKWHDAGHKGLRLAINLSMQQFSRPDLPPRLAAMIAAQGIDASC
                     VDVEITEYMLFRDALANHAAARALRDIGVGVVVDDFGTGACSIAHLSRSPATAVKIDL
                     SFVAGLTSRAADRGVCAAMIALAHELGRAVAAEGVETAAQADVLREQGCDLLEGFLFL
                     KPSPAPAIGAFLAARSQG"
BASE COUNT          779 a         1588 c         1636 g          815 t
ORIGIN      
        1 gttccgagca ggccgtccgc gccggcgcgc gtgagcgtga acgtcgcggt cgtgacggac
       61 ggcgcgttca gctcgcggtc gaagccgacg acgatcgaag caggcagcgc gagcatcttc
      121 gagctcggct ccggcgtgac cgacgtgacg cggatcggcc ccaagcctgc cgccgcgggc
      181 agcgcgccgt cgccgatcca ttggcgaatg acgttgatgt cgctctgcgg cagaggaggc
      241 aggccggccg gcatgcgctc gccgacggcg gcagtgcctt cgagcttctg gatgaggtag
      301 ctattgttcg cgtcgccggg cgcgacgcgc agcacgcccg agcgctcgcc gctcggaact
      361 ccgacgaggc tcgcgtagct gttcgcggcg tcgagccgca gcccgaccgg cgcgttcgcg
      421 cccgagtggc agtgctcgca gtactggtcg aagacgttcg cttggatcga gttgaagctc
      481 ggctcgagcg gaccgacgac gccgccgctg cccacgaacc cgtcgccgga gccgcacgcc
      541 gcgagcgcgc agcaggcgag gggcgccacc atgcggcgcg ctgccgcatg ccgccgttcg
      601 aatagtcgca tgtcgatttc ccactgtgac cgtctggcta agtcccgggc acgacagcgg
      661 aaggttcaac gcgatcgcgg cgctgcgcgc gcggcgtcgc aggcgggcga ccagagcggg
      721 gcgcgattca gcgcgcgagc ggctcgacga gcgtgatcat ggcctggtgc gtgtggccga
      781 gccacaggcc cgataggcca ttgcgctcgg cgggtttcaa gttgatgtcg cggatgttcg
      841 cttcgaacgg caacagatag aaattccatt cgccggtctc gacgttcatg cgcgcgacgc
      901 gatcggcata gatgccggct gtccaggtgt agccctgctc gtcgtaatag gcgtcgtacg
      961 ggaagaagta cgggaacggc accgcgtaca atttcatctc ctcgcctttc ggcttgcgcg
     1021 gatcgagcat tccgacgaag ccgccgtcga agccgcccca ccagagccga tcttgcttgt
     1081 ccgtgatgcc gcgcctcatg cccgtgccga acgagccggc accgcgcggc tcaacgggaa
     1141 tgtcgaagta ctgcacggca tgcgtcttcg cgtcgacgcg ccagacgtag ggcgcgttgc
     1201 gcgcgctgcc gtagacgttg ttcttcgagt cggcggcgat gtcgtacgcg tagagcggct
     1261 tctcgttcgt gcccttgatc tcgacgtgct gccaggtgtc gagatcgatc tgatagacgg
     1321 cgcctccggc gacgggtgcg gcgacggagc gccacgcttt gccgtcgacg cgcgcaccgg
     1381 aaacgtcgat catctcgccg ccgccgggcg ggtcgtgata ttcgaactgc tccgtcttgg
     1441 gatcgaacac gacgacgccg ctttgcgcct gattgccgat gtaccagcgc ccgcgcgcgt
     1501 cgggccccat cagcgtgcgc gcgcccgtcg gctggaacga gccttccttg gcgcgatagg
     1561 gccagtgcca ttctttgact tcgccggttt tcggatcgag cctgccgatc gcgttgtcgt
     1621 tgaagtggtt gaaccagatc atgccgtcgg cggcgatgcg cgtgtcgtgc ggccgtgccg
     1681 tcgcgggcaa atcccacgtc gtatacacga cctcggtctc tttgcccttc ggccgcggca
     1741 tcgtcttgag ctcgtacggc cacatgccgc tccgcagatt gatcgacgcg acggcctcgg
     1801 cttggcgctt ctgcagcgcg gacggctcgc cgccctcggt gcggccccaa tgcggcacca
     1861 agttggccgg cagctggaag cgcatcggcg agctgccggc cgaatagacc ctgcgcatca
     1921 ggacccacga cagctcctcg gcgtcgtagg tcgacatcgc cggccggcgc agcgtgtggc
     1981 agcggctgca gtcgcggaac agctcccatt tcgtgcgatc ctcgatgggc cagctcgcga
     2041 gccactcggg atccgtcagc tgcagcgcga gctcgagcgg attcgcgctc gtaagctcga
     2101 gatcgagccg cgccggcttg ccggccgcaa cgtcgacgct cgctgcgtgc ttcggcagca
     2161 cgtacctcac ggcgcgaacg gtgacggcgt agcggcccgg ctcgaggcgg tcgctcggga
     2221 acgaatagcg gccgtcggcg tggctgacga ccgtgacggt cttgttcgag ccttcgcgct
     2281 tcgcgctgac gagcacgcct tccatcgcgc cttcggccga cgacgtcacg cggccggaaa
     2341 gtgcagcctg ttgagcgacg accgcctgtg ccaagaacga tgcgctcgct acagccgcaa
     2401 gcgtcgaagc gcgaacgaac agactcagcc gcatgaaatc ccccaagaca gctcggtctc
     2461 ggtggacagt gtaacgacat cttcgtcccg ccagtgggcg ggaacggcgc ccgtcggcga
     2521 gagcgccgac gagcactacg gatctgcgtc tgctagggct tgcagacgcg cggggtcacg
     2581 cgtattcgat cgagggcgaa gtcgaagaac gcgaccgcag tcgcgctcgt atcgagcttg
     2641 gcgcggaaaa agccaaaggc ggaggtgtga tccttttcct gcaggtcgtc gagctcgaac
     2701 gcttggttca ggccgaagcc gtcgatgaag tcgagcggcg accggatgcc gatcgtgagc
     2761 tgctcgccgg agagcctgtc gtccgccgag ctcgggccga accggtccga gacttcaaac
     2821 cagtccgcat gcaacaggtc cggcgcccgc aggcggacgt cgtcgccgac gatgacgtag
     2881 tcgtccgcaa cgctgcccgg gaacggcgac acttgatgcg tgatgtcgcc tacggggccc
     2941 ttgggaaacg gagaaagcca gcggctcgtc acgaatgacg ccccgcttgg agggttaccg
     3001 ccggttacct ggccgtagat ctttccatcg aaagagacgc cgcccgtagc cctcggcagc
     3061 ggcgcatcgt tggcggagat gcggaaggtg ccatgcaccg gatcgccgaa ggtgatgacg
     3121 tcgttcgttg gatcccttgg atctgggttg acgagacgtt gtcgtcccca gaattcgacg
     3181 tcgatcgcga ttctcgactc cccgacgacg acttgagcgg cggaagcggt tcccgatgcg
     3241 agggcagccg cggtgagagc gccgattccc aacaatgtcc tcatgacccc actcccttgg
     3301 ttgttgagga caagaaagat gcacttgtcg tgccagtcaa accgtcatcg gtaacgtcga
     3361 cgtatcgaga gatcgtgcgc gacgaaaggc cgcggaggtc tgtcgcggcg tcaaccctgg
     3421 ctgcgcgccg ccaggaacgc gccgatggcc ggcgcggggc tcggcttcag aaacaggaat
     3481 ccttcgagga ggtcgcagcc ctgctcgcgc agcacgtccg cctgcgcggc cgtttcgacg
     3541 ccctcggcgg cgaccgcgcg gccgagctcg tgcgcgagcg cgatcatcgc cgcgcacact
     3601 ccgcggtcgg cggcgcggct cgtcaggccg gcaacgaagc tcaaatcgat cttgaccgcc
     3661 gtcgcggggc tccgcgagag atgcgcgatc gagcacgcgc ccgtgccgaa gtcatcgacg
     3721 acgaccccga cgccgatgtc gcgcagcgca cgggccgcgg cgtggttcgc gagcgcgtcg
     3781 cggaacaaca tgtattccgt gatctcgacg tcgacgcagc tcgcgtcgat gccttgcgcc
     3841 gcgatcatcg ccgcgagacg cggcggcaag tcgggccgcg agaactgctg catcgagaga
     3901 ttgatcgcga gccggagccc cttatgcccc gcgtcgtgcc acttcctcag gtcactgaag
     3961 ctccgctgca tgacccattc gccgatcggc acgatgagcc cggtgtgctc ggcgagcgcg
     4021 acgactttct gcgtgcgctg cgtgccgaag gccgcctgcg gccagcgcaa caacgcttcg
     4081 acggcggcga cgctgcccga cctcgcgttc acgataggta gatattccag atggaattgc
     4141 tcgcgttcga gcgcggtctt gagctccagc tcgagctcgt tgcgctgcag cgcgcgcagt
     4201 ttggaggtgc ccgagtggaa cttgtagctg tcggtctggc tgctgcgcgc gtcttccatc
     4261 gcggtgagcg cattctgaaa caacgtgtcg acgtcggcac cgtcctgcgg aaacagcgcg
     4321 atcccggctc gcgcggtcac cgcgacttgg cggtccccga cctcgaccgg cagctcgaga
     4381 gcctctacga gccgtaacgc cacgctctcg gcgtctgcgc cgctctgaat gctcggcagc
     4441 agcacgccgt attgcctgta gtcgatgcgc gcagcgaccg agtaacgctc gtagtcgagc
     4501 tcgctgtggc tgttcacgcc gcgcaacgag agcacgaggc gactcgcgag ctccttcagc
     4561 acgaggtcct ggctctggcc gctgaatgcg ttggcgtgcc cctcgctttg ctcgatgctg
     4621 aggcaaatga cggcggcgcg gagctctttg agccgtgcga tgtcggtaaa gcgcgcgagc
     4681 tcttcaagga agtactcgcg cgtcggcagc gtcgtggccc ggtccgtgta cgcgagatcg
     4741 agcatgcgcg aataggcatt cgcgggatcg ccgacgtcga gcacgacgac gagcgcgctg
     4801 tcgcggccgt gcggcacg
//
LOCUS       DASSNN010000019         5541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_884931, whole genome shotgun sequence.
ACCESSION   DASSNN010000019 DASSNN010000000
VERSION     DASSNN010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884931"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1287
                     /locus_tag="VFL84_00325"
     CDS             157..1287
                     /locus_tag="VFL84_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607092.1"
                     /translation="MATGCAHRTLLVLAISGSAAAAAQEPAPVSVSALRSVPAETTAA
                     AHVDKGWRAPRTSWGHPSFEGSWSTDDFRGVPLSRPREQGERTSLTAEEFAKRAQEIE
                     SGRDFSVNVGTFLRHEFGLRTFGYTSMIVDPPDGQLPQLTPHGEELAKQRTRGTFGPG
                     PFDDLDDFSLYDRCITRGVLGSLLPVIYGNGVRITQNPDSVAISYEMIHDTRIIPLDG
                     RPALDNGIRQYMGSARGRFEGDTLVVETTNFTDKTSVGTNGGGPPNSEKLKLTERFTR
                     VDPAMIEYVATVDDPGAFTAPWTIRLMITSQPGYEVFEYSCHEGNGAVAHALSGERAY
                     ELQVAEAKAKGLPIPARAVEHEQIRNGVPGEGQHVFDINAGE"
     gene            1295..1522
                     /locus_tag="VFL84_00330"
     CDS             1295..1522
                     /locus_tag="VFL84_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607093.1"
                     /translation="MVRRAFDIVMRIVTSLVGLLMILMGSVWMMQGLGVGPAAIMQGF
                     MVNDIQWTFYGAILVVVGIAKIVWSLLRAKV"
     gene            complement(1519..2043)
                     /locus_tag="VFL84_00335"
     CDS             complement(1519..2043)
                     /locus_tag="VFL84_00335"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HET7607094.1"
                     /translation="MKKQQLDHVLRAAGRITGETQFVIVGSQSLHGRYPDAADEIVHS
                     AEVDLIAKNAPQRTEWLNAIGQDSPFHEEFGYYADPVDETTATLPRGWKGRLVNLPPG
                     DTEGVRGLCLDPHDLAISKYVARREKDRTFTRELARRALVDRERLLALVDKTPVGEDV
                     RKRIRAYIAADFAD"
     gene            complement(2024..2461)
                     /locus_tag="VFL84_00340"
     CDS             complement(2024..2461)
                     /locus_tag="VFL84_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET7607095.1"
                     /translation="MRLTIRRIGNSLGVIIPRSTLDAWGLTEGDTLELTARSIGPPRR
                     LDAHDALDEHKRKLAAAVATRFPANAIRAHSLSNLHRWREAGAWVSAYDEWTDILRSG
                     DDGALFAAMLGRDERANRLRQSPPYVGLLPRDEVAKLNEEAAA"
     gene            complement(2714..2869)
                     /locus_tag="VFL84_00345"
     CDS             complement(2714..2869)
                     /locus_tag="VFL84_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607096.1"
                     /translation="MTYALMLAAAFAHGVLRARDERGGQSDGDLGLRHAADHTAALRR
                     QIHICDS"
     gene            3063..4463
                     /locus_tag="VFL84_00350"
     CDS             3063..4463
                     /locus_tag="VFL84_00350"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7607097.1"
                     /translation="MRTCVFLVLACLAGGAHAQAPSDAERAAALEIYRKIVSLDTSVD
                     GGETPAMASYLADRFRAAGFAEEDVHVLPLPSTAALAVRYRGDGTQRGALLLLAHMDV
                     VPARRSEWERDPFTLIEEDGFFFGRGSADNKSGVALITATFLRLRAAGFKPTRDVVLW
                     FSGDEETTGATTMEMLAKHRDWLGDVELALNSDAGGGTLAHDGRATAYMLQTAEKTYA
                     DFKLTARNPGGHSSMPRADNAIFDLMDALGRIRGFEFPVMWNDTTIASFRSTGKEVGG
                     DDGAAMLRFAQRPGDAEAAATLSRNPVYVGQVRTTCVPTMLAAGHATNALPQTATANV
                     NCRIFPGVAPAAVQAELQRLAGEAVEVEQDGDVHFSDASPLRADVVAAVTAAVHARYP
                     GIPITPAMEVGATDGLFFRAAGIPTYGVSELFIRDEDLFMHGLNERNPVASFYDGLRH
                     WRVLIEALAGPNAGSR"
     gene            4549..5157
                     /locus_tag="VFL84_00355"
     CDS             4549..5157
                     /locus_tag="VFL84_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607098.1"
                     /translation="MANGQDNADFSLENAASTPGGDVGGPVSPPPHWLERGGITRGMV
                     GSLTPAIYNAGNEIVQSPGYVVIRNEMIHEARVVPLDGRPPLAAAIKQWVGDSRGHFD
                     GDTLVVETTNFTDRTNVGQARHSDKLKITERYLRVADDKLVLRLTVEDPETWAAPWTL
                     ELPLDRDDSYGMFEYACHEGNLAMFNILSGARADERAAARER"
     gene            complement(5167..5481)
                     /locus_tag="VFL84_00360"
     CDS             complement(5167..5481)
                     /locus_tag="VFL84_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607099.1"
                     /translation="MSEKFVDVLEHELAEEKAGALGRCARLLEQALERFRRHDETHER
                     PDVDAKRERLLWDLAERVEAFVVQREACGLRDSRHALKFYGVPREAIARVGARRPVRL
                     TR"
BASE COUNT          893 a         1831 c         1905 g          912 t
ORIGIN      
        1 gttcgagcgc ttgctcggtc cgccgaccaa ggttgcgcgg accggcacgg accgataggc
       61 gacgcaacct cgcgctcgcg ccagcgctcc gatgaagctg ggcgcgcgca acatcggtta
      121 tagtcaggcc ggtcgctccc aaagaggggt cacggcatgg ctacgggctg cgcacatcga
      181 acgctgcttg ttttggctat ctcgggctcg gcggccgccg cagcccaaga gccggctccc
      241 gtcagcgtct cggcgctgcg ctccgtgccg gcggagacga cggccgccgc ccacgtcgac
      301 aagggctggc gcgcgccgcg cacgtcgtgg ggccacccga gcttcgaagg cagttggagc
      361 accgacgatt tccgcggcgt gccgctgagc cggccgcgcg agcagggcga gcggacgtcg
      421 ctgaccgccg aggagttcgc gaagcgcgcg caggagatcg agagcggccg cgacttctcg
      481 gtcaacgtcg gcacgttctt gcggcacgag ttcggcctgc gcacgttcgg ttatacgtcg
      541 atgatcgtcg atcccccgga tggccagctg cctcagttga cgccgcacgg cgaggagctc
      601 gcgaagcagc gaacgcgcgg cacgttcggg cctgggccat tcgacgactt ggacgacttc
      661 tcgctgtacg accgctgcat cacgcgcggc gtgctcggct cgttgctgcc cgtcatctac
      721 ggcaacggcg tgcgcatcac gcagaacccc gactccgtgg cgatcagcta cgagatgatc
      781 cacgacactc gaatcattcc gctcgacggc cggcccgcgc tcgacaacgg catccgccag
      841 tacatgggca gcgcgcgcgg ccgattcgaa ggcgacacgc tcgtcgtcga gacgacgaac
      901 ttcacggaca agacgagcgt cggcacgaac ggcggcggcc cgccgaacag cgagaagctg
      961 aagctgaccg agcgcttcac gcgcgtcgat cccgcgatga tcgagtacgt cgcgaccgtc
     1021 gacgatcccg gcgcgttcac ggcgccgtgg acgattcggc tcatgatcac gtcgcagccc
     1081 ggctacgagg tgttcgagta ctcgtgccat gaaggcaacg gcgccgtcgc gcacgcattg
     1141 agcggcgagc gcgcgtacga gctgcaggtc gcggaagcga aggcgaaagg cttaccgatc
     1201 cctgcgcgcg ccgtcgagca tgagcagatt cggaacggcg tgccaggcga aggccagcac
     1261 gtcttcgaca tcaacgcggg cgagtagcgg agagatggtg cggcgcgcgt tcgacatcgt
     1321 catgcggatc gtcacgagcc tcgtcggcct gctgatgatc ctcatgggct cggtctggat
     1381 gatgcagggt ttgggcgtcg gccccgcggc gatcatgcag gggttcatgg tcaacgacat
     1441 ccagtggacc ttctacggcg cgatcctcgt cgtcgtcgga attgccaaga tcgtctggag
     1501 cttactgcgc gccaaagtct aatcggcgaa gtcggctgcg atgtaagcgc ggatgcgttt
     1561 gcgaacgtct tcgccgacgg gcgtcttgtc tacgagcgcg agtagtcgct cgcggtcgac
     1621 aagcgcgcgc cgcgcgagct cgcgagtgaa agtgcgatcc ttctcgcgcc gcgcgacgta
     1681 tttcgagatt gcgaggtcgt gcggatcgag gcacaggccg cgcacaccct cggtgtcgcc
     1741 gggcggcaga ttcacgagtc gacccttcca gccgcgcggc aacgtggccg tcgtttcgtc
     1801 cacgggatcc gcgtagtaac cgaattcctc gtgaaacggc gaatcttgtc cgatggcatt
     1861 gagccattcg gtgcgctgtg gagcgttctt ggcgatcaag tcgacttccg ccgaatgcac
     1921 gatctcgtcc gcggcgtcgg gatagcgccc gtgcagcgac tggctgccga cgatcacgaa
     1981 ttgcgtctcg cccgtgatgc ggccggccgc gcgcagcacg tgatcaagct gctgcttctt
     2041 cattgagctt cgctacttcg tcacgcggta gcagaccgac gtaaggtggg gactggcgca
     2101 gccgattcgc gcgctcgtct ctgcccagca tcgcggcgaa cagggcgccg tcgtcgccgc
     2161 tgcgcagtat gtccgtccat tcatcgtatg cggacaccca cgcgccggcc tcgcgccagc
     2221 gatgtaggtt cgagaggctg tgcgcgcgga tcgcattcgc ggggaaacgc gtggcgacgg
     2281 cggccgcgag cttgcgcttg tgctcgtcga gcgcatcgtg cgcgtcaagt cggcgaggag
     2341 ggccgatgct gcgagccgtg agctccagcg tatcgccctc ggttaggccc cacgcgtcga
     2401 gcgtcgatct cgggatgatg acgcccaagg agttgccgat gcgccggatc gtgagtcgca
     2461 tgcgcacgtt atatcatgtt ataacagttg ccggtcaaga ggcgccgcgg tttgcgattc
     2521 ggaggctctc ctagacgccg ggcctgacgt tcgtcgtcgt cgcgatgctc gcgtgcttgg
     2581 tgccggcggc gcgggcggct gctacgccca aggttgatgc gttgcgctca ggctgatgca
     2641 tctccatagc gtccgcggcg ccgtccaggt ccgcaaattt tcgaccgagg cgttgcgata
     2701 cgaataaccg gtctcagctg tcgcatatgt ggatttgccg cctcagtgcc gcggtatgat
     2761 ccgcggcatg acgaaggcca agatcgccgt cactttgccc cccgcgctcg tcgcgcgcgc
     2821 gcagcacgcc gtgcgcgaag gccgcggcga gcatcagcgc gtacgtcacg tcggccctcc
     2881 aggaaaaggc gaagctcgac gagctcgaga cgatgctcga cgagatgctt gcagagacgg
     2941 gcgtgcctct cactgatcgt gagcaggctg cggccgacgc tgcgctgggc gtcgcatccg
     3001 ctagaaaacg cagacgccgg cgtaaacgcg cggcctttac gcactcgagg ggtggatagc
     3061 tcatgagaac gtgtgtcttt ctcgtgctcg cgtgcctcgc cggcggcgcg cacgcgcaag
     3121 cgccgagcga cgcggagcgc gccgcggcgc tcgagatcta ccgcaagatc gtctcgttgg
     3181 atacgtcggt cgacggcgga gagacgccgg ccatggcgag ctatctcgcg gataggtttc
     3241 gagcggcggg gttcgccgaa gaggacgtgc acgtgctgcc gctcccgagc acggcggcgc
     3301 tcgcggtgcg gtatcgcggc gacggcacgc agcgcggcgc gcttcttttg ctcgcgcaca
     3361 tggacgtcgt gcccgcacgg cgcagcgagt gggagaggga tccgttcacg ttgatcgagg
     3421 aggacgggtt cttcttcggt cgcggctcgg ccgacaacaa gtccggtgtc gcgctgatca
     3481 ccgcaacttt tctgcgcttg cgcgcggccg gcttcaagcc gacgcgcgac gtcgtgctct
     3541 ggttctcggg cgacgaggag acgaccggcg cgacgacgat ggagatgctc gcgaagcatc
     3601 gcgattggct cggcgacgtc gagctcgcgt tgaactcgga tgccggcggc ggcacgctcg
     3661 cgcacgatgg ccgcgcgacc gcgtacatgc tgcagaccgc cgagaagacc tacgcggact
     3721 tcaagctgac ggcgcgcaat ccgggcggcc actcgtcgat gccgcgcgcc gataacgcga
     3781 tcttcgatct catggacgcg ctcggccgca ttcgcggctt cgagttcccc gtgatgtgga
     3841 acgacacgac gatcgcgagc ttccgcagca cgggcaagga agtcggcggc gacgacggcg
     3901 ccgcgatgct gcgtttcgcg caaaggcccg gggacgcgga agcggccgcc acgctgtcgc
     3961 gcaatcccgt gtacgtgggg caggtgcgca cgacgtgcgt gcccacgatg ctcgcggccg
     4021 ggcatgcgac gaatgcgttg ccgcagacgg cgaccgcgaa cgtgaactgc cggatctttc
     4081 ccggcgtcgc gccggccgcc gtgcaggccg agctgcagcg gctcgccggc gaagccgtcg
     4141 aggtcgagca ggacggggac gtccacttca gcgacgcctc gccgctgcgc gccgacgtcg
     4201 tcgccgccgt gacggccgcc gtgcacgctc gctatcccgg catacccatc acgccggcga
     4261 tggaggtcgg cgcgacggac gggttgtttt tccgcgccgc cggcatcccg acttacggtg
     4321 tcagcgagct cttcatacgc gacgaggatc tgttcatgca cggcttgaac gaacggaacc
     4381 ctgtcgcgag cttttacgac ggccttcgcc actggcgcgt gctgatcgaa gcgctcgcgg
     4441 ggccgaacgc gggctcgcgg tagctcgttc cgttgcgccg cgcggtcgcg cggcggcaag
     4501 atgggccgaa cgaccgatgg ggggcggcat gcggatactc gaacggtctt ggctaacggc
     4561 caggacaacg ccgacttctc gctcgagaac gcggcctcga cgccgggcgg cgacgtcggc
     4621 ggccccgtct cgccgccgcc gcattggctc gagcgcggcg gcatcacgcg cggcatggtc
     4681 ggctcgttga cgccggcgat ctacaacgcg ggcaacgaga tcgtgcagtc gccgggctac
     4741 gtcgtgatcc gcaacgagat gatccacgaa gcccgcgtcg tgccgctcga cggccgcccg
     4801 ccgctcgcgg ccgccatcaa gcagtgggtc ggcgactcgc gcgggcactt cgacggcgac
     4861 acgctcgtcg tcgagacgac gaacttcacg gaccgcacga acgtcggcca ggcgcgccac
     4921 agcgacaagc tcaagatcac ggagcgctac ctgcgcgtcg ccgacgacaa gctcgtgcta
     4981 cgcctcacgg tcgaggatcc cgaaacgtgg gccgcaccgt ggacgctcga gttgcctttg
     5041 gaccgcgacg attcttacgg catgttcgag tacgcgtgcc acgagggaaa tctcgcgatg
     5101 ttcaacatct tgagcggcgc gcgcgccgac gagcgcgcgg cggcgcgaga gcgctgaaga
     5161 cgccgtctat cgcgtgagtc gaacggggcg tctcgcgccg acacgcgcga tcgcctcgcg
     5221 cggcacgccg tagaacttca gagcgtggcg cgagtcgcgc aggccgcacg cctcgcgctg
     5281 aacgacgaac gcctcgacgc gctcggcgag gtcccaaagc agccgctcgc gtttcgcatc
     5341 cacatccggc cgctcgtgcg tttcgtcgtg acggcggaac cgttcgagcg cctgctcgag
     5401 taaccgggcg caacggccca gcgcgccggc cttctcttcg gcgagctcgt gctcgaggac
     5461 gtcgacgaac ttttcgctca tgccgtgcgc gtcattctac tggcttcccg cggcccgatg
     5521 ggtatgactt ctcccaattc t
//
LOCUS       DASSNN010000020         5837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1263162, whole genome shotgun sequence.
ACCESSION   DASSNN010000020 DASSNN010000000
VERSION     DASSNN010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263162"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VFL84_00365"
     CDS             complement(<1..842)
                     /locus_tag="VFL84_00365"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HET7607100.1"
                     /translation="MKQYRFHHRILLLAVALVFTTQLVMLFPVLDLIKRDSDAQADRT
                     VGLAGALFDQYIQNRSEGQLTNVNLLASDYAFKQTVVSENDAATIRSLLRNHMGRVGA
                     NVAAVLDLDGAVRVSESVDDRTVSAFPSATFAAIEDGARHNVLNIGGVPYQTFTVPLR
                     APDLRAYVMLGFPIDFALASQLKELTGLDVSFLSSSAVEPRVLTSTLPVEARQNALAG
                     LDLNVTGPQRAGKGAAAHVSLARPFDEGSDDVYVVMQLSQSVATASYRRVRDSLYAIT
                     GVSLL"
     gene            952..1647
                     /locus_tag="VFL84_00370"
     CDS             952..1647
                     /locus_tag="VFL84_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HET7607101.1"
                     /translation="MRTTAFVRVACLRLGSLLAALAPAAYSAAATLEISVVDEQGRPI
                     DDVAVYATPAHAFDHDGAGAAHAAPAPKAAMDQQHLQFVPHLLVVQTGTEVSFPNSDD
                     VSHHVYSFSETKPFELALYKGDQYPPVTFDRPGVVVLGCNIHDGMLGYIVVVDSPHFA
                     KTDERGIALIDDIASGDYNVAVWTPRARPAALPPALQLTVGEAGVAAEIRITGRLSPA
                     HEHNVSSLTWDRY"
     gene            1632..2918
                     /locus_tag="VFL84_00375"
     CDS             1632..2918
                     /locus_tag="VFL84_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607102.1"
                     /translation="MGPILTRSGPAAALAAVCAALSVSAQSERSDRNELYLGASAGYV
                     SASTDLNAWTEGGFSKLRYADDGFEAFRLFGEYHGRITPTLRARVVADYVDDASGGID
                     FTEAVLDWRPIPRSRNQQQLRFGAFYPPFSLENGDRGWQSPFTYSSSAINTWLGEEIR
                     PFGAEWSLRHRFDGFRSNHEVRAFAAGFYGNDPAGTLLFWRGWSLHDRQSRFNDELDI
                     PPRPFTSAPPRKLEPFEEIDHRPGVYAGVEWRYARRALVQLARYDNRADPFAFSDGQW
                     AWGTDFDHLAVQLDLPAQLGLIVQWMEGYTEWLTAALPDGTRLPISEHAHDEFESSFV
                     LLTRKLGAAQRLAVRYDTFSYERPGAVPELYGDEGDAWTLSYRIEPTRRFSGGIEWLR
                     IDSRRDLWPMFYFMPAEQSESQIRLQLSYRLAAPAR"
     gene            3015..3995
                     /locus_tag="VFL84_00380"
     CDS             3015..3995
                     /locus_tag="VFL84_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET7607103.1"
                     /translation="MKDVIVVGASAGGIRALKGVLAGLPRELPASVLAVVHIPRHGTS
                     VLPLILDAAGPLPAKAAEDGEALARGTVYVAPPDRHLLVEGGRLRLTRGPRENRVRPC
                     VDALFRSAAVDLGPRVVGVVLSGTLDDGTAGLWAIKDRGGVAVVQQPDEAEYPDMPAS
                     ALANVEVDHVLRVAEMGPKLAALALEKSEAQAPVPDAMAAEAEIELGGHALQKGVLEL
                     GVPSPNSCPECRGSLVEIREGPIVRYRCHTGHAYSLQTLLAETDADIEKALWNAVRAI
                     EERSFLRKAMERRAREAGDVALADRLAADAARDVADAQRVRRLTAGPAAA"
     gene            4164..>5837
                     /locus_tag="VFL84_00385"
     CDS             4164..>5837
                     /locus_tag="VFL84_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET7607104.1"
                     /translation="MADNEHNAATRLTVVGIGASAGGIDALRTFFSAVPADLGVAYVV
                     VVHLAPDYESELASILGRSTKMSVVEVSDHEELELKPNCVYVIAPDRKLVLTPKTVAA
                     APFSDLRERRAAIDVFFRSLAESHGDGFAIVLSGGGSDGSLGARAVKETGGLVLVQDP
                     REATHEAMPRAVIAAELADVVAPVAELASRLVELVRHRRTLAPLIRAPREAVTESDEA
                     VLRRFFELVRARTGHDFTRYKRPTILRRLARRMQLNHRADFEHYLALLRESTEEIQAL
                     FDDLLISVTAFFRDAGTWEALRSQVVVPLVERAHGEESIRVWVPGCATGEEAYTLAIL
                     FREEIARRDVACELVVFGSDVDQSALATAREGVYPAAIAADVSEARLARYFRAEGDHF
                     RVTNEIRDSVVFAVHSVLRDPPFSKLHLISCRNLLIYLDRELQQQLQNVFRYGLRDDG
                     FLLIGVSETADPDLFEAVDKHNRIFRARARSPGPPVRLPQLPDVPHVPPTIERVRDRN
                     VRARRSAAETHIEALEELAPPSILVDEHWNVEHLSASAGRFLQQRGGPPT"
BASE COUNT          880 a         2004 c         2000 g          953 t
ORIGIN      
        1 agcagcgaca cgcccgtgat cgcgtagagg gaatcgcgca cacgccggta cgaagccgtc
       61 gcgaccgatt gagagagctg catgacgacg tagacgtcgt cgctgccctc gtcgaacggc
      121 cgcgcgagcg agacgtgcgc ggcggcgccc ttgccggcgc gctgcgggcc ggtgacgttc
      181 aagtccagcc cggcgagcgc gttctgtcgc gcctcgaccg gcagcgtcga ggtcaggacg
      241 cgcggctcga ccgcgctcga ggacaggaac gagacgtcga ggcccgtgag ctccttcagc
      301 tgcgacgcga gcgcgaagtc gatcgggaag ccgagcatga cgtacgcgcg cagatcgggc
      361 gcgcgcagcg gcaccgtgaa cgtttgatac gggacgccgc cgatgttgag cacgttatgg
      421 cgggcgccgt cctcgatcgc ggcgaatgtg gcgctcggaa aggcggacac ggtccggtcg
      481 tcgacggact cgctgactct gacggctcca tccaagtcga gcacggcggc gacgttggcg
      541 ccaacgcggc ccatgtgatt gcgcagcaac gagcgaatcg tggcggcgtc attctcgctg
      601 acgacggttt gcttgaaggc atagtccgac gcgagcaagt tgacgttcgt cagctgcccc
      661 tcggaacggt tctggatgta ctggtcgaac agcgcgccgg cgaggccgac cgtgcgatcg
      721 gcctgcgcgt cggagtcgcg tttgatgaga tcgagaaccg gaaacagcat cacgagttgc
      781 gtcgtgaaca cgagcgcaac ggccagcagc agaattctgt gatgaaacct gtactgtttc
      841 attcagttgc agatattacg ttcgagactc ttgaggttgt gtgcggccga tcacggttgc
      901 gcttcgagcg tcgacataac attcggactc cttacttatt gcgaagcttg aatgcgcacg
      961 accgcgttcg tccgcgtcgc ctgcttgcga ttgggctcgc tgctcgcagc gctcgcgccg
     1021 gccgcgtatt cggcggcggc gacgctcgag atctccgtcg tcgacgagca aggcaggccg
     1081 atcgacgacg tcgccgtata tgcgacgccc gcgcacgcgt tcgatcatga cggtgccggc
     1141 gcggcgcatg cagcgcccgc gcccaaagcg gccatggatc agcagcacct gcagttcgtg
     1201 ccgcatctgc tcgtcgtgca aacgggcacc gaggtttcgt tcccgaacag cgacgacgtc
     1261 agccatcacg tctactcgtt ctcggagacg aagccgttcg agctggctct gtacaagggc
     1321 gaccaatacc cgccggtcac gttcgaccgg cccggtgtcg tcgtgctcgg ctgcaacatc
     1381 cacgacggca tgctcggcta catcgtcgtc gtcgattcgc cgcacttcgc gaagaccgac
     1441 gagcggggaa tcgcgctgat cgacgacatc gcgagcggcg actacaacgt cgcggtctgg
     1501 acgccgcgcg cgcggcccgc tgcgttgccg ccggcactgc aactcacggt cggcgaagcc
     1561 ggggtcgcgg ccgagattcg catcacgggc cggttgtcgc cggcgcacga gcacaacgtt
     1621 tcgagcctca catgggaccg atactgacgc ggagcgggcc ggcggccgcg ctcgcggcgg
     1681 tttgcgccgc gctctccgtc tcggcccaga gcgagcgcag cgaccgcaac gagctctatc
     1741 tcggcgcttc ggccggctat gtctccgctt ccaccgacct gaacgcgtgg accgaaggcg
     1801 gcttcagcaa gctgcgctac gccgacgacg gcttcgaggc gttccgcctg ttcggcgaat
     1861 accacggccg catcacgccg acgctgcgcg cgcgcgtggt cgccgactac gtcgacgacg
     1921 cgtccggcgg catcgacttc acggaggcgg tcctcgactg gcgcccgatc ccgcgctcgc
     1981 gcaatcagca gcagctgcgc ttcggcgcgt tctatccgcc gttctcgctc gagaacggcg
     2041 accgcggctg gcagagcccg ttcacctact cgagctcggc gatcaacacg tggctcggcg
     2101 aggagatccg gccgttcggc gcggagtggt cgctgcgtca ccgcttcgac ggcttccgca
     2161 gcaatcacga ggtgcgcgcg ttcgcggccg ggttctacgg caacgacccg gcgggcacgc
     2221 tgctcttctg gcgcggctgg tctctgcacg atcggcaatc gcgcttcaac gacgagctcg
     2281 atattccgcc gcgcccgttc acgagcgcgc cgccgcggaa gctcgagccg ttcgaggaga
     2341 tcgatcatcg tcccggcgtc tacgccggcg tcgaatggcg ctacgcgcgc cgcgcgctcg
     2401 tccagctcgc gcgttacgac aatcgcgcgg acccgtttgc gttctcggac ggccaatggg
     2461 cctggggcac cgacttcgac cacctcgcgg tgcagctcga ccttcccgcc cagctcgggc
     2521 tcatcgtgca atggatggaa ggctacacgg agtggctgac cgcggccttg cccgacggca
     2581 ctcgcctgcc gatctcggag cacgcgcacg acgagttcga gtcgagcttc gtcctgctca
     2641 cgcgcaagct tggtgcagcg cagcgcctcg ccgtgcgcta cgacacgttc tcgtacgagc
     2701 ggcccggcgc cgtccctgag ctctacggcg acgagggcga cgcgtggacg ctgagctatc
     2761 ggatcgagcc gacgcggcgc ttcagcggcg gcatcgaatg gctgcgcatc gattcgcgcc
     2821 gcgacctctg gccgatgttc tacttcatgc ccgccgagca atcggagagc cagatccgct
     2881 tgcagctctc gtaccgcctc gccgcgccgg cgcgttagcg ccgtcggcgt tcgatggcat
     2941 gtcccttgca ctgagctgac cctacgcgtc gcgcctgcac cccgccggcg ttgcgtcgct
     3001 ggaggggcag ggctatgaag gacgtgatcg tcgtcggcgc gtcggcgggc gggatccgcg
     3061 cgctgaaggg tgtcctcgcc ggtctgccgc gcgagctgcc cgcctcggtt ctcgccgtcg
     3121 tccacatccc gaggcatggg actagcgtct tgccgctcat tttggacgcg gccggaccgt
     3181 tgccggccaa agcggccgag gacggcgagg ctctcgcgag gggcaccgtt tacgtcgcgc
     3241 cgcccgatcg ccatctgctc gtcgaaggcg gccgtctgcg gctcacgcgc ggcccgcgcg
     3301 agaatcgcgt gcggccgtgc gtcgacgcgt tgttccggtc ggccgcggtc gacttgggcc
     3361 cgcgcgtcgt cggcgtcgtg ctgtccggca cgctcgacga cggcacggcc ggtctctggg
     3421 cgatcaagga tcgcggcggc gtcgcagtcg tccagcaacc ggacgaggcc gagtatcccg
     3481 acatgccggc gagcgcgctc gcgaacgtcg aggtcgatca cgtgctgcgc gtggccgaaa
     3541 tgggaccgaa gctcgccgcg ctggcgctgg agaagtccga ggcgcaggcg cccgtgccgg
     3601 acgcgatggc cgccgaagcg gaaatcgagc tcggcggcca cgcgctgcag aaaggcgtgc
     3661 tcgagcttgg cgtgccgtcg ccgaactcgt gccccgaatg ccgcggttcg ctcgtcgaga
     3721 tccgtgaagg cccgatcgtg cgttatcgct gccacacggg ccacgcctac tcgctgcaaa
     3781 cgctgctcgc cgagaccgac gccgatatcg aaaaagcgct gtggaatgcc gtgcgcgcga
     3841 tcgaggagcg gtcgtttctg cggaaggcca tggagcgccg cgcgcgcgag gccggcgacg
     3901 tggcgctggc cgaccggctg gcggccgacg cggcccgcga cgtggccgat gcccaacgtg
     3961 ttcggcggct gacggccggg cctgccgccg cctagcgacg cggcattcag tatttatccg
     4021 tagttgctat gttctccgtg ccaggcggac aatccgggca gaggcgcccc tcggggaaca
     4081 aggcgcgccg catagcaaag agcctcgaaa cggatcggtc gtttgggccg aacgatgcga
     4141 ctttcatcga agggcccgtg cgcatggcgg acaacgagca caacgcggcg acgcgtctga
     4201 cggtcgtcgg catcggagcg tctgcgggcg gcatcgatgc gctgcgcacg ttcttcagcg
     4261 cggttccggc cgatctcggc gtcgcgtacg tcgtcgtcgt gcacctcgcg ccggactacg
     4321 agagcgagct cgcttcgatt ctcggccgca gcacgaagat gagcgtcgtc gaggtcagcg
     4381 accacgagga gctcgagctc aagccgaact gcgtctacgt tatcgcgccc gaccgcaagc
     4441 tcgtgctgac gccgaagacg gtcgccgccg ccccgttcag cgaccttcgc gagcggcgcg
     4501 ccgcgatcga cgtgttcttt cgttccttgg ccgagagcca cggcgacggg ttcgcgatcg
     4561 tgctctcggg cggcggctcg gacggctcgc tcggcgcgcg cgccgtcaag gaaaccggcg
     4621 gcctcgtgct cgttcaggac ccgcgcgaag cgacgcacga ggccatgccg cgcgccgtca
     4681 tcgccgccga gctcgccgac gtcgtagcgc ccgtcgcgga gctcgcctcg cggctcgtcg
     4741 agctcgtgcg gcatcggcga acgttggcgc cgctgatccg cgcgccgcgc gaggcggtta
     4801 ccgagagcga cgaggccgtg ctgcggcgct tcttcgagct cgttcgcgcg cggaccggcc
     4861 acgacttcac gcgctacaag cggccgacga tcctgcggcg gctcgcgcgg cgcatgcagc
     4921 tgaaccaccg cgcggacttc gagcactatc tcgcgctgct gcgcgagagc acggaagaga
     4981 tacaggcgtt gttcgacgat ctgctgatct cggtcaccgc gttcttccgc gacgccggta
     5041 cctgggaggc gctgcgctct caggtcgtcg tgccgctcgt cgagcgcgcg cacggcgaag
     5101 agtcgatccg cgtctgggtg ccgggctgtg ccacgggcga ggaagcgtac acgctcgcga
     5161 tcctgttccg cgaggagatc gcgcggcgcg acgtcgcctg cgagctcgtg gtcttcggct
     5221 ccgacgtcga ccaaagcgcg ctcgcgacgg cgcgcgaagg cgtgtatccg gccgcgatcg
     5281 ccgccgacgt ctccgaagcg cggctcgcgc gctatttccg cgccgagggc gaccacttcc
     5341 gcgtcacgaa cgagatccgc gactccgtcg tgttcgcggt gcacagcgtg ctgcgcgatc
     5401 cgccgttctc gaagctgcac ttgatctcgt gccgcaattt gctcatttat ctcgaccgcg
     5461 agctgcagca gcaactgcag aacgtgtttc gctacggcct gcgcgacgac ggcttcctgt
     5521 tgatcggcgt ctccgagacg gccgatcccg acttgttcga ggccgtggac aagcacaatc
     5581 ggatttttcg cgcgcgcgca cgctcgcccg ggccgccggt gcgtttgccg cagctgcccg
     5641 acgtgccgca cgtcccgccg acgatcgagc gcgtgcgtga ccgcaatgtg cgggcgcgcc
     5701 gctcggcggc cgagacgcac atcgaagcgc tcgaagagct cgcgccgccg agcatcctcg
     5761 tcgacgagca ctggaacgtc gagcacttgt ccgccagcgc ggggcgtttc ttgcagcagc
     5821 gcggcggtcc gccgacg
//
LOCUS       DASSNN010000021         4845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_632919, whole genome shotgun sequence.
ACCESSION   DASSNN010000021 DASSNN010000000
VERSION     DASSNN010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632919"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VFL84_00390"
     CDS             complement(<1..818)
                     /locus_tag="VFL84_00390"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HET7607105.1"
                     /translation="MKRQLGYGLVVAATLALLGGCDSFGAKQAAYGSPEQAGEALLAA
                     VRSGETSEMLRVLGEAAQPVVESGDAVDDANTRSEFLAAYDAAHRWVNQTPEIATLVV
                     GDDDWTFPFPLVNDDDGWRFDTTYGIEEILDRRIGENELAAIQASLAYVDAQREYYWS
                     NPDGAPMLHYARNLVSTPGEKDGLFWESAEDEAPSPLGALFAAARSEGYLLDGTTRPV
                     PYYGYHFRILTAQGEHAAGGAYDYVVGDLMIGGFGLVAYPAERGTSGVMTFIVN"
     gene            complement(818..2335)
                     /locus_tag="VFL84_00395"
     CDS             complement(818..2335)
                     /locus_tag="VFL84_00395"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HET7607106.1"
                     /translation="MPRARLDSLFRAAVPPGIAALMLAVQTPSAQQSPGIAPPTPVGG
                     PPAPAAPASATPTAAKSGTFSTEELEQMAAPIALYPDPLLAQVLMASTYPLEVVEAAR
                     WKKANASLTGDAVAEALKKEDWDASVKSLVTFPDVLAMMDEQIGWTQKLGDAFLAQQP
                     ELMDSVQRLRAKAKAEGTLMSSKEQNVIEEPAPPPPAGAVAPAAAAAAPAPTTIIKIE
                     PSDPEVVYVPTYNPTVVYGAWPYPAYPPYYYYPPMYPPGAAFFSFTAGVIVGGALWGS
                     CGWGWGHSEVNINVNRYNNFTGSVGGGGLGGVGGVGGVGGVGGVGGVGGAGGVGGVGG
                     AGGIGGAGQPKWQHNPEHRKGAQYRDSATQDKFGRGQSSNSVQSREQFRGRAEQGRRD
                     IAAGGATRDLAGNQLGGANASNRLGGGQAGAGNRAGTQPSAGNRAGNTASTRPSAGTS
                     NRSSSGRSSSAFGGTSSGSSSRSAGQRGYSSRSAGGMSRGGGFSGGGFRGGGGRR"
     gene            complement(2427..4184)
                     /locus_tag="VFL84_00400"
     CDS             complement(2427..4184)
                     /locus_tag="VFL84_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7607107.1"
                     /translation="MTRRSMPFGLAILALLAAQVAAAQRAITPVTDAMLRNPDPADWL
                     MWRRTLDSWGYSPLDDIDRGNVARLALAWSVDLDAAPSQEGIPLVHDGVLYFPSPSDV
                     TTAFDAATGAKLWEHRRDLPADLGQFVPFPQTNRNLAIYDRLIIDNGADDFVYALDAA
                     TGKQVWETKILDYKIHHVKQGSAPLVANGVLIAGRNCQPNGGPDACVITGHDARTGQE
                     LWRKRTIPKPGEPGGDSWGDVPDEKRWHVGAWMIPSYDPELDLVYIGTSVTAPAPKFM
                     LAGNDKQYLYHNSTLALRPKTGETVWYYQHAVDHWDLDHPFERILVDTAVAPDASEVP
                     WINPRLRAGERRRVVTGIPGKTGLVYTLDRVTGEFLWARPTITQNVIERIDGATGAVA
                     IRADALFTGPSQSRLICPTTNGGKNWPAGAYSPRTKTMYFAMANTCAWTTSAATATPT
                     PEMIYAIATENVMAPDAGGKAGTLRAISVETGKQDWRYDQRAALMALLATGGDLVFGG
                     DVAGLLRAFDATTGEVLWQFGLGASVTGHPVTFAVAGKQYVAVSTGRSNVTGGLTRLT
                     PEAAPADSANKLFVFALPD"
     gene            complement(4181..>4845)
                     /locus_tag="VFL84_00405"
     CDS             complement(4181..>4845)
                     /locus_tag="VFL84_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7607108.1"
                     /translation="ERCYMRPGDFIVTPSWTWHEHRNEGAAPTIWLDVLDVPLIRFLG
                     AGFSEHYLEPEHPQTGPPGDSHFRYGRNLVPVGFERGGASPVFAYPFEHAYEALDHLR
                     AHSEWDPWHGLKMQYIDPTSGGPAIPTISTFLQLLPQGFATQPYRATASSIVTVVRGR
                     GSVTVGRGAGAVTFEYGPKDLWAVPSWQHVSVSAVEESVLFSASDEAVHRKLGVWREQ
                     RA"
BASE COUNT          700 a         1677 c         1680 g          788 t
ORIGIN      
        1 tgattgacga tgaacgtcat gacgccgctc gtgccgcgct cggctggata cgcgacgagg
       61 ccgaacccgc cgatcatgag gtcgccgacg acgtagtcgt aggctccgcc cgcggcgtgc
      121 tcgccttgcg ccgtcagaat gcggaagtgg tatccgtaat acggcaccgg ccgtgtcgtg
      181 ccgtcgagga gatagccttc cgagcgcgcg gcggcaaaca gcgcgccgag cgggctcggg
      241 gcttcgtctt cggcgctctc ccagaacagc ccgtccttct cgcccggcgt gctgacgagg
      301 ttgcgcgcgt agtgcagcat cggcgcgccg tcgggattcg accagtaata ctcgcgttgc
      361 gcgtcgacgt aggcgagcga cgcctggatc gcggcgagct cgttctcgcc gatgcgccga
      421 tccaagattt cttcgatgcc gtacgtcgtg tcgaagcgcc aaccgtcgtc gtcgttgacg
      481 agcgggaacg ggaatgtcca atcgtcgtcg ccgacgacca gcgtcgcgat ctcgggcgtt
      541 tgattgaccc agcgatgcgc cgcgtcgtac gcggcgagaa actcgctccg cgtgttcgcg
      601 tcgtcgaccg cgtcgcccga ctccacgacc ggctgagccg cttcgccgag cacgcgcagc
      661 atttcgctcg tctcgccgga ccgcacggcc gcgagcagag cctcgccggc ttgctcgggt
      721 gagccgtagg cggcctgctt tgcgccgaac gagtcgcagc cgccgagaag cgcgagcgtc
      781 gccgcgacga cgaggccgta gccgagttgt ctcttcatca gcgccgccct ccgccgccgc
      841 ggaaaccgcc tccggagaag ccgcctccgc gagacatgcc gccggcgctg cggctcgagt
      901 agccgcgctg gcccgcggaa cgcgacgagc tgccgctgct cgtgccgccg aaggcgctcg
      961 agctgcgccc ggacgagctg cgattcgaag tgccggccga cggccgcgtc gaagccgtgt
     1021 tgccggctcg attgccggcg ctcggctgcg tgccggcccg attgccggct ccggcctgtc
     1081 cgccgccgag ccgattgctg gcattcgcgc cgccgagctg gttgccggcg aggtcgcgag
     1141 tcgcgccgcc cgccgcgatg tctcggcggc cttgctccgc gcggccgcgg aactgctcgc
     1201 gcgactgcac ggaattcgag ctctgaccgc ggccgaactt gtcttgcgtc gcggaatcgc
     1261 ggtactgcgc gcctttgcgg tgctcgggat tgtgctgcca tttcggttgg ccagcgccgc
     1321 cgattccgcc agctccgccc acaccgccga ccccgccggc gccaccaacc ccgcccactc
     1381 cgccgacgcc gccgacgcca ccgacgccgc cgacgccgcc aagtccgccg ccaccgacgc
     1441 tgccggtgaa gttgttgtag cggttgacgt tgatgttgac ttcgctgtga ccccaacccc
     1501 aaccgcagct gccccacaag gcgccgccga cgatgacgcc ggccgtgaag ctgaaaaacg
     1561 ccgcgcccgg cggatacatc ggcgggtaat agtaataggg cgggtaggcg gggtacggcc
     1621 aagcgccgta cacgaccgtg gggttgtagg tcggcacgta cacgacctcg gggtcggacg
     1681 gctcgatctt gatgatcgtc gtcggcgcgg gcgcggcggc ggcagcggca ggcgcaacgg
     1741 cgcccgcggg cggcggaggc gccggctcct cgatgacgtt ctgctccttg ctcgacatca
     1801 gcgtgccctc ggctttcgcc ttggcgcgaa gtctctgcac ggagtccatc agctcgggct
     1861 gttgcgcgag gaacgcgtcg ccgagcttct gcgtccagcc gatctgctcg tccatcatcg
     1921 cgaggacgtc gggaaacgtc acgagcgatt tgacgctcgc atcccagtcc tcctttttca
     1981 gagcttccgc gacggcgtca ccggtcagcg acgcgttcgc cttcttccag cgcgccgctt
     2041 cgacgacctc gagcggatac gtcgacgcca tcagcacctg tgcaagcagc gggtcgggat
     2101 agagcgcgat cggcgccgcc atctgctcga gctcctcggt cgagaacgtg ccggatttgg
     2161 ccgccgtcgg cgtggccgaa gcgggcgcgg ccggagccgg cggccctccg acaggcgtcg
     2221 gcggggcgat ccccggcgac tgctgtgccg acggcgtctg gaccgcaagc atcaacgcgg
     2281 cgataccggg cggcacggca gcgcgaaaga gcgaatcgag acgggctctg ggcataggtt
     2341 tctccccgcg acactatcgc cagggcgcgc ccgcgccggt aataggacct taggccacga
     2401 ggggatccgt gctcgtcgcg tcggcgctag tccggcagcg cgaacacgaa tagcttgttt
     2461 gcgctgtcgg cgggcgcggc ctccggcgta agccgcgtga gcccgcccgt cacgttcgag
     2521 cggccggtgc tgacggcgac gtactgcttg ccggcgaccg cgaacgtcac gggatggccc
     2581 gtgacggaag cgccgaggcc gaactgccag agcacctcgc cggtcgtcgc gtcgaaggcg
     2641 cgcaacaagc cggcgacgtc gccgccgaac acgaggtcgc cgcccgtcgc gagcagcgcc
     2701 atcagcgcgg cgcgttggtc gtagcgccag tcttgcttgc ccgtctcgac cgagatcgcg
     2761 cgcagcgtgc cggctttgcc gccggcgtcg ggtgccatga cgttctcggt cgcgatcgcg
     2821 tagatcatct ccggtgtcgg cgtcgccgtc gcggccgacg tcgtccacgc gcacgtgttc
     2881 gccatggcga agtacatcgt cttcgtgcgc gggctgtacg cgccggccgg ccagttcttg
     2941 ccgccgttcg tcgtcggaca gatgagccgg ctctggctcg gccccgtgaa caacgcgtcg
     3001 gcgcggatcg cgacggcgcc ggtggcgccg tcgatgcgct cgatgacgtt ttgcgtgatc
     3061 gtcgggcgcg cccagaggaa ttctcccgtc acgcggtcga gcgtgtagac gaggcccgtc
     3121 ttgccgggga tgccggtcac gacgcgccgc cgctcgccgg cgcgcagacg cgggtttatc
     3181 cacggcacct cgctcgcgtc gggcgcgacc gccgtgtcga cgagaatgcg ctcgaacgga
     3241 tgatcgagat cccagtgatc gacggcgtgc tggtagtacc agaccgtctc gccggtcttc
     3301 ggccgcagcg cgagcgtcga gttgtgatag aggtactgct tgtcgttgcc ggcgagcatg
     3361 aacttcggcg ccggcgccgt caccgacgtg ccgatgtata cgagatcgag ctcggggtcg
     3421 tagctcggga tcatccaggc tccgacgtgc caccgcttct cgtcgggcac gtcgccccag
     3481 ctgtcgccgc cgggctcgcc cggtttcggt atcgtgcgct tgcgccagag ctcctggccc
     3541 gtgcgcgcgt cgtggccggt gatgacgcac gcgtcgggcc cgccgttcgg ctggcaattg
     3601 cggcccgcga tcaagacgcc gttcgcgacc agcggcgccg acccttgctt cacgtggtgg
     3661 atcttgtagt cgaggatctt ggtttcccaa acctgcttgc cggtcgcggc atcgagcgcg
     3721 tagacgaagt cgtcggcgcc gttgtcgatg atgagccggt cgtagatcgc gagattgcga
     3781 ttcgtctgcg ggaacggcac gaactgcccg agatcggcgg gcaagtcgcg gcggtgctcc
     3841 cagagcttcg cgcccgtcgc cgcatcgaac gcggtcgtga cgtccgacgg gctcgggaag
     3901 tacagcacgc cgtcgtgcac gagcgggatg ccttcctgcg aaggtgcggc gtcgagatcg
     3961 acggaccacg cgagcgcaag ccttgcgaca ttgccgcggt cgatgtcgtc caacgggcta
     4021 taaccccagc tgtcgagcgt gcgccgccac atcagccagt cggcgggatc gggattgcgc
     4081 agcatcgcgt cggtgacggg cgtgatcgcg cgctgcgccg ccgcgacctg cgcggcgaga
     4141 agcgcgagga tcgccaaacc gaacggcatc gaccgtctcg tcatgctcgc tgctcgcgcc
     4201 acacgccgag cttgcggtgc acggcctcgt cggacgcgct gaatagcacc gactcctcga
     4261 ccgcgctcac gctgacgtgc tgccagctcg gcacggccca aagatccttg gggccatact
     4321 cgaacgtgac cgcgccggcg cctcggccga ccgtcacgct gccgcggccg cgcacgaccg
     4381 tgacgatcga gctcgcggtg gcgcggtacg gctgcgttgc gaacccctgt ggcaggagtt
     4441 gcaggaacgt cgagatcgtc ggaattgccg ggccgcccga ggtcgggtcg atgtactgca
     4501 tcttgaggcc gtgccacggg tcccactcgc tgtgggcgcg cagatggtcg agcgcctcgt
     4561 acgcgtgctc gaacgggtaa gcgaacaccg gcgacgcgcc gccgcgctcg aagccgaccg
     4621 gaacgagatt gcgtccgtag cgaaagtggc tgtcgcccgg aggtccggtt tgcggatgct
     4681 cgggctcgag atagtgctcc gagaatccgg ctcccaggaa acggatcagc ggcacgtcga
     4741 gcacgtcgag ccagatcgtc ggcgccgcgc cttcgttgcg gtgctcgtgc cacgtccacg
     4801 acggcgtcac gatgaaatcg ccggggcgca tatagcagcg ctcgc
//
LOCUS       DASSNN010000022         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1013582, whole genome shotgun sequence.
ACCESSION   DASSNN010000022 DASSNN010000000
VERSION     DASSNN010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013582"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="VFL84_00410"
     CDS             complement(<1..1388)
                     /locus_tag="VFL84_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7607109.1"
                     /translation="MARIAATPADLVFGNTHAQLAQIVDQTAAAVFVKDLAGRLIFAN
                     PAFESITGLPLDAIVGRLDRDVFPSTAAELHGNDRRVVETGQAMDFEETIATVDGLRT
                     YLSHKFPLFDESGAVCAVCGIATDITERKRVEEALRAAALAVSTVEGASVFGELVRSL
                     ATILRVDAAMIAVFVDGDSTRMRTLAARLDGNTLRNFEYPLAGSPCRDVVGREFRYVG
                     SGVNREFPPGTLFAAKAMDSYAALPLTGANGRPLGLIAAMDRQPMRDAALAEAMLKIF
                     AVRAAAEIERTLGEAQLRQSQKMEAIGHLTGGIAHDFNNLLTSIMGYVALAAERVTGA
                     DPKLATYLDQATASCLRARDLIQQMLTFSRGGRGDPQVIALAPRIGEALRLLRASLPA
                     TVAMRMELDDDAPPVLLDPVQLEQVLMNLVINARDAMRSSGELRVTLHKAAAAAVCTS
                     CRTPFSGEWVELA"
     gene            1630..>2190
                     /locus_tag="VFL84_00415"
     CDS             1630..>2190
                     /locus_tag="VFL84_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607110.1"
                     /translation="MQAAATLGASQRYAKAVEVSKRIRWDIDRDVVRGRTFDFARKFL
                     PDALSRADRVDSLSASEKRLLSQIQGRTYANMFGLVERFIGAKLLELSRDHWLGDQAA
                     LEALVRFTDEELKHQELFRRIERMIGDGMPAGYRFVPHPNEVARAVLGKATWSVLALT
                     TMIELFTQAHYRSSIDPDADLSPLWKD"
BASE COUNT          365 a          755 c          732 g          338 t
ORIGIN      
        1 gccagctcga cccactcgcc gctgaacggc gtgcggcaac tcgtgcacac ggccgcggcc
       61 gcggctttgt gcagcgtcac gcggagctcc ccgctcgatc gcatggcgtc gcgggcgttg
      121 atcaccagat tcatcagcac ctgttcgagc tgcaccgggt cgagcagcac cggcggcgca
      181 tcgtcatcga gctccatccg catcgcgaca gtggcgggca gcgaggcgcg cagcagccgg
      241 agcgcctcgc cgatgcgcgg cgcgagcgcg atcacctgcg gatcgccgcg accgccgcgg
      301 ctgaacgtga gcatctgctg gatcaggtcg cgggcacgaa gacacgaggc ggtcgcctgg
      361 tccagatagg tggcgagctt cggatccgcg cctgtcacgc gctccgccgc gagcgccacg
      421 taccccatga tgctggtgag cagattgttg aagtcgtgcg cgatgccgcc ggtcaagtgc
      481 ccgatcgcct ccatcttctg cgactgacgc agttgcgcct cgccgagcgt ccgctcgatt
      541 tcggcggccg cgcgcacggc gaagatcttg agcatcgcct ccgcgagcgc tgcatcgcgc
      601 atcggctgcc ggtccatcgc cgcgatgaga ccgagcgggc gcccgttggc gccggtgagc
      661 ggcagcgcgg cgtagctgtc catcgctttt gcggcgaaca gcgtgcctgg cgggaactcc
      721 cggttcacgc cgctgccgac gtagcggaac tcgcgcccga cgacgtcgcg gcacggcgag
      781 ccggcgagcg gatactcgaa attgcgcagc gtgttgccgt cgagccgtgc ggcgagcgtg
      841 cgcatcctcg tcgagtcgcc gtcgacgaac acggcgatca ttgccgcatc gacgcgcagg
      901 atcgtcgcga gcgagcgcac gagctcgccg aacacgctcg cgccttcgac ggtggacacg
      961 gcgagcgccg ctgcgcgcag cgcctcctcg acacgcttgc gctcggtgat gtcggtcgcg
     1021 attccgcaca cggcgcagac cgcgcccgat tcgtcgaaca atggaaactt gtgcgacaga
     1081 tacgtgcgca agccgtccac cgtcgcgatc gtctcctcga agtccatcgc ctgtccggtc
     1141 tccacgacgc gccggtcgtt gccatgcaac tccgcggcgg tggatgggaa gacgtcgcga
     1201 tcgaggcgac cgacgattgc gtcgagcggc aggccggtga tcgactcgaa cgccggattc
     1261 gcgaagatca gccggccggc gagatccttg acgaaaaccg cggctgccgt ctggtccacg
     1321 atctgcgcga gctgggcgtg cgtgttgccg aaaacgaggt cggcaggcgt tgcggcgatt
     1381 cgagccatga cgcgaacccg gggggaaatc gcagcagtct accggcccgc ggggtgaccg
     1441 cgcgactgta gctgagggct acacgcgaaa tccgcgcgct gcggaccgcc gtcgatacac
     1501 atgcaacaaa tccgcgtccg tctgttgccc ggcggaaatc atcccgacac gtgcgcttgg
     1561 cacactgcgc acgtgcctcg gacggcaacc ggccaggccc gcacgacatc attccacggg
     1621 agagatttca tgcaagcagc cgccaccctc ggcgcctcgc agcggtacgc gaaggccgtt
     1681 gaagtttcga agcgcattcg ctgggacatc gaccgcgacg tcgtcagggg acgcaccttc
     1741 gacttcgcca ggaagttcct gcccgacgct ctgtccaggg ccgatcgggt cgacagcctg
     1801 tccgcatccg aaaagcggct gctgtcgcag atccagggcc ggacgtacgc caacatgttc
     1861 gggctcgtcg agcgcttcat cggcgccaag ctgctcgagc tgagccgcga ccactggctc
     1921 ggcgaccagg ccgcgctcga ggcgctcgtg cgcttcaccg acgaggagct caagcaccag
     1981 gagctgttcc gccgcatcga gcgaatgatt ggcgacggaa tgcccgccgg ctaccggttc
     2041 gtcccgcatc cgaacgaagt cgcgcgtgcc gtgctcggca aggcgacgtg gtcggtgctc
     2101 gcgctcacga cgatgatcga gctcttcacg caggcgcact accggtcgag catcgacccc
     2161 gatgccgatc tgtcgccgct gtggaaagac
//
LOCUS       DASSNN010000023         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_255432, whole genome shotgun sequence.
ACCESSION   DASSNN010000023 DASSNN010000000
VERSION     DASSNN010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255432"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1052)
                     /locus_tag="VFL84_00420"
     CDS             complement(198..1052)
                     /locus_tag="VFL84_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET7607111.1"
                     /translation="MNEVQTFVLEDSGIRGALVRLEETWQRVVAAHDYPPEVRALLGD
                     GVVSTLLLASGLKTTPSVSLQLQGEGPLKLLLVQCTDGKRVRGMASFKTYAAGTPLLG
                     DGRLVVNLDTGKPNGFFQGIVPLTSAKLEECLESYFAKSEQLDTRLVLRSDGERVAGL
                     LLQVLPGHGEERAVEAFETAAACAATVSAGELMRSTAEDLLPKLFAGQAIRLFEARSV
                     AHDCRCTPERLAGVIRMLGADEVKSLLAEQGHVELKCEFCNRAFRYDDAQIDAIMRGA
                     ATESSAVH"
     gene            complement(1063..>2197)
                     /gene="gatB"
                     /locus_tag="VFL84_00425"
     CDS             complement(1063..>2197)
                     /gene="gatB"
                     /locus_tag="VFL84_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET7607112.1"
                     /translation="VDVVLEDGSTRRVGIVRAHLEEDAGKSLHEDFEGMSGIDLNRAG
                     TGLLEIVSAPDLRSAAEAGAYMRKIHALVRYLGICDGNMQEGSFRCDANVSVRKRGEA
                     KLGTRTELKNLNSFRFVEKAIEIEAERQIELIESGGKVKQETRLYDPDRNETRPMRSK
                     EEANDYRYFPDPDLLPVSIDDRLVEEVRASLPELPDFKRERFMRDYALPAYDAQVLTV
                     ERALADYFETVAKSTKAAPKIAANWVMGELTGALNREGLDVGAARVSAAALASLLDKL
                     AAGTISGKIAKDVFEAVWRGEGSVDSIIEKRGLTQISDSASIDKLVADVIAANPAQVE
                     QFRAGKQQVLGFLVGQVMKASRGKANPQQVNEALRKQLGPPSN"
BASE COUNT          323 a          757 c          725 g          392 t
ORIGIN      
        1 gccgcggtcg cgcttgcgct cgtcgtcgga cagcgccgcg cacgccgcgt acagcctgcc
       61 gttcatccac gcgttgtact cggccagcag ccgcatttca tcgctacgca tcgcctctcc
      121 caagaggtgc cggggttacg gttcggttca accgaagtgc cggagttacg gttcaaccga
      181 aaccgtaaca ccggcaccta gtggacggca gaagattccg ttgcggcgcc gcgcatgatc
      241 gcgtcgatct gagcgtcgtc gtagcggaat gcgcggttgc agaattcgca tttgagctcg
      301 acgtggcctt gctccgcgag caggctcttg acctcgtcgg cgccgagcat gcgaatgacg
      361 ccggccaaac gttccggcgt gcagcggcag tcgtgcgcga cggagcgcgc ctcgaacagg
      421 cgaatcgcct ggcccgcgaa cagcttcggc aagaggtcct cggcagtcga gcgcatcagc
      481 tcgccggcgg atacggtggc cgcgcatgcg gccgcggtct cgaacgcctc gaccgcgcgc
      541 tcctcgccgt ggcccggcaa gacttgcagc agcagccccg cgacgcgctc accgtcggag
      601 cgcagcacga gccgtgtgtc gagctgttcc gatttcgcga agtacgactc gaggcattcc
      661 tcgagcttcg cgctcgtcag cggcacgatg ccttggaaga atccgttcgg cttgccggtg
      721 tcgaggttga cgacgagccg gccgtcgccg agcagcggcg tccccgcggc gtacgtcttg
      781 aacgacgcca tgccgcgcac gcgcttaccg tccgtgcatt ggacgagcag cagcttcagc
      841 ggcccttcgc cctgcagctg cagcgacacg ctcggcgtcg tcttgagccc gctcgcgagc
      901 agcagcgtcg acacgacgcc gtcgcccagc agcgcgcgca cctcgggcgg gtagtcgtgc
      961 gccgcgacga cgcgctgcca cgtttcctcg agccgcacga gcgcgccgcg gatgccgctg
     1021 tcctcgagca cgaaagtctg gacctcgttc atggcgggtt agtcaattcg acggcggccc
     1081 gagttgcttg cgcagagcct cgttgacctg ctgcggattc gccttgccgc gcgacgcttt
     1141 catgacctgg ccaacgagga agccgagcac ttgctgcttg ccggcgcgga actgctcgac
     1201 ctgcgccggg ttcgccgcga tgacgtcggc gacgagcttg tcgatgctcg cggagtccga
     1261 gatctgcgtg aggccgcgct tctcgatgat cgagtcgacg ctgccttcgc cacgccacac
     1321 cgcttcgaag acgtccttcg cgatcttgcc cgagatcgtg ccggccgcga gcttgtcgag
     1381 cagcgaggcg agcgcggccg ccgagactcg cgcggcgccg acgtcgagcc cttcgcgatt
     1441 cagcgcgccc gtcagctcgc ccatgaccca gttcgcggcg atcttcggcg ccgccttcgt
     1501 gctcttcgcg acggtttcga aatagtcggc gagcgcgcgc tcgacggtca gcacttgcgc
     1561 gtcgtaggcg ggcagggcgt agtcgcgcat gaaccgctcg cgcttgaagt cgggcagctc
     1621 gggcagcgac gcgcgcactt cctcgacgag ccggtcgtcg atcgagacgg gcagaagatc
     1681 gggatcgggg aagtagcggt agtcgttcgc ttcctccttg ctgcgcatcg gccgcgtctc
     1741 gttgcggtcc ggatcgtaga gccgcgtctc ctgcttgacc ttgccgccgc tctcgatgag
     1801 ctcgatctgc cgctcggcct cgatctcgat cgctttctcg acgaaccgga acgagttcag
     1861 attcttgagc tcggtgcgcg tgccgagctt cgcttcgccg cgcttgcgca ccgacacgtt
     1921 ggcgtcgcag cggaacgagc cttcctgcat gttgccgtcg cagatcccga gatagcgcac
     1981 gagcgcgtgg atcttgcgca tgtaggcgcc ggcctcggcg gccgagcgca ggtcgggagc
     2041 ggagacgatc tcgagcaggc cggtccccgc gcgattcaga tcgatgcccg acattccctc
     2101 gaagtcctcg tgcagcgatt tgccggcgtc ctcttcgagg tgcgcgcgca cgatgccgac
     2161 tctgcgcgtg ctgccgtcct cgagcacgac gtcgacc
//
LOCUS       DASSNN010000024         4229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1767587, whole genome shotgun sequence.
ACCESSION   DASSNN010000024 DASSNN010000000
VERSION     DASSNN010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767587"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="VFL84_00430"
     CDS             <1..1401
                     /locus_tag="VFL84_00430"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7607113.1"
                     /translation="WEHRRKMPDDVGQYFPAAETNRNLAIYDNLILDNSADGYAYALD
                     ARTGEQVWETKILDYRTGAKNSSGPIIVNGKVVSGRSCEPEGGPEACVITAFDAKTGR
                     ELWRTRTIPKPGEPGDETWGDIPYEKRWHVGMWMVPSFDPELNRIYVGTSVTAPAPKY
                     MLGGNQEQHLYHNSTLALDADTGKIVWYFQHVVDHWDLDHPFERLLVDTVVAPDPKSV
                     RWINPRVRSGERRKVITGIPGKTGIVYTLDRETGDFLWARETTMQNVISDINVESGRA
                     TVNPDKIFTAPGQEKLICPSTSGGKNYPAGTYSPLTDVMFYPLNNTCMSATSGNNPVD
                     TDVTYALRNRVQITPGTDKVGVIEAISVRTGETVWKREQRAGQLSLMSTAGGLLFGGD
                     ANGRFRAYDQRSGDVLWEINLGAPVNGYPVTFAVDGKQYVAVSTGGSGLALGFVSLTP
                     ELKPGLRNQLFVFALP"
     gene            1526..2797
                     /locus_tag="VFL84_00435"
     CDS             1526..2797
                     /locus_tag="VFL84_00435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7607114.1"
                     /translation="MQATASEASRNGILTTLGATVGLALGPSVIANLTVTGYITPIEQ
                     EFGWLRSDVSFAFSLVAYMIVAMSPLQGVLVDRFGPRRVVLTSIPLFALSLAAIYFTP
                     ANMYVYYLLWAIVPVASIGLWPLGYLQAVTPWFDRKLGLALGCANAGIGVGSTFLPML
                     VLGPMIAAYGWREAVLSVAALVLFVSWPIVAYCLREPSAVEAAARKLTAAGKSFGLSF
                     QQSMREPTFWILNIGFFLLGVTATSLVTQQVPLLRDAGWTAAETTRLQTIFGFGLLFA
                     RVAVGFVIDHIFAPRVLTTVSIGGAVACLLYALYPEYGYVSAILIGFLLGAEFDVLAF
                     LIKRYYGNVAYGRVYGVIFGVFYLGSAVGIWGLPKLRELSVDLSYDKPLYAAAAILLA
                     SAVLMAFMPKYRYAAGHTVAASAGTPAAQQA"
     gene            2815..>4229
                     /locus_tag="VFL84_00440"
     CDS             2815..>4229
                     /locus_tag="VFL84_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7607115.1"
                     /translation="MRPLLLKTSALLVAVAICGGAAAQQRTLRPVTDEMLQNPDPADW
                     LSWRRTLNHWGYSPLTEINKRNVAQLRLVWTRPLAVGVQEGTPLVHDGVMFFPNPNDI
                     TQALDAATGDLIWEYRRPVQADNNDYIPFPSINRNLAIYGNLILDNGADNYAYALDAA
                     TGELKWETQILDYRHGAQHSSGPIVANGKVISGRSCEPEGGPEACVLTAFDAATGNEL
                     WRTRTIPKPDEPGGDTWGNIPYEERRHVGLWMVPSFDPELRRIYVGTSVTSPAPKFML
                     AGNDKQYLYHNSTLAIDVDTGKIVWYLQHNVDHWDLDHPFERILIDTEVAPDPDAVAW
                     INPRVRRGEMHKVITGIPGKTGLVYTLDRETGQFLWSRPTVQQNVITRVNTESGEGVV
                     NTEKLFTGPNQEILVCPSLTGGKNYHAGAYSPDTKLIYMPLQNVCMTATSTASQPSLD
                     ELYAVRTKDQIAPGEQNVGTI"
BASE COUNT          753 a         1414 c         1342 g          720 t
ORIGIN      
        1 tgggagcatc ggcgcaagat gccggacgac gtcggccaat atttcccggc cgcggaaacc
       61 aaccgcaatc tcgcgatcta cgacaacttg atcctcgaca acagcgcgga cggttatgcc
      121 tacgcgctcg acgcacgcac cggcgagcag gtctgggaaa cgaagatcct cgattaccgc
      181 accggcgcga agaacagctc gggcccgatc atcgtgaacg gcaaggtcgt ctcgggacga
      241 agctgcgagc ccgagggcgg gcccgaggcc tgcgtcatca cggcgttcga cgcgaagacc
      301 ggccgcgagc tatggcgcac gcgcacgatc ccgaaacccg gcgagcccgg cgacgagact
      361 tggggcgaca tcccgtacga gaagcgctgg cacgtcggca tgtggatggt gccgagcttc
      421 gacccggagc tgaaccgcat ttacgtcggc acgtcggtca cggcgcccgc gccgaagtac
      481 atgctcggcg gcaatcagga gcagcacctc tatcacaact cgacgctcgc gctcgacgcc
      541 gacacgggca agatcgtctg gtacttccag cacgtcgtcg atcattggga tctcgaccat
      601 ccgttcgagc gcctgctcgt cgacacggtc gtcgcgccgg atccgaagtc ggtgcggtgg
      661 atcaatccgc gcgtacgcag cggcgagcgg cgcaaggtca tcaccggcat tccgggcaag
      721 accggcatcg tctacacgct cgatcgcgag acgggcgact tcttgtgggc gcgcgagacg
      781 acgatgcaga acgtcatctc cgacatcaac gtcgagtcgg gccgcgccac cgtgaacccg
      841 gacaagatct tcacggcgcc gggtcaagaa aagctgatct gcccgagcac gtcgggcggc
      901 aaaaattacc cggccggcac gtacagcccg ctgacggacg tcatgttcta tccgctgaac
      961 aacacctgca tgtcggcgac gtccggcaac aaccccgtcg ataccgacgt cacctacgcg
     1021 ctgcgcaacc gcgtgcagat cacgcccggc acggacaagg tcggcgtcat cgaggcgatc
     1081 tcggtgcgca ccggcgagac ggtctggaag cgcgagcagc gcgcgggaca actctctctg
     1141 atgtcgacgg ccggcggcct cctgttcggc ggcgacgcca acggccggtt ccgcgcctac
     1201 gaccagcgct ccggcgacgt gctctgggag atcaatctcg gcgcgcccgt caacggctat
     1261 cccgtcacgt tcgcggtcga cggcaagcag tacgtggcgg tcagcacggg cggctcgggc
     1321 ctcgcgctcg gcttcgtgag cttgacgccg gagctgaagc ccggactgcg caatcagctg
     1381 ttcgtgttcg ctctgccgta gactcgtgcc cgcgacccgg cagccttaca aatggggaca
     1441 gatctatttt ctttcgaaaa tcgatctatc cccgggccgg gccgacgccg gccattaata
     1501 acgcatcact ctcgggggat ttgagatgca agccacagca agcgaagcct cgcgcaacgg
     1561 aattctgacg acgctcggcg ccacggtcgg gctcgcgctc ggtccgagcg tcatcgcgaa
     1621 cttgacggtg accggctaca tcacaccgat cgagcaggag ttcggctggc tacgctccga
     1681 cgtgtcgttc gcgttctcgc tcgtcgccta catgatcgtc gcgatgtcgc cgctgcaagg
     1741 agtgctcgtc gaccggttcg gcccgcgccg cgtcgtgctg acgtcgattc cgttgttcgc
     1801 gctgagcctg gcggcgatct atttcacgcc cgccaacatg tacgtctact acctgctctg
     1861 ggccatcgtg ccggtggcca gcatcggcct ctggccgctt ggctacctgc aggccgtgac
     1921 gccctggttc gatcgcaagc tcgggctcgc gctcggctgc gccaacgccg gcatcggcgt
     1981 cggcagcacc ttcctgccga tgcttgtgct cggcccgatg atcgcggcgt acggctggcg
     2041 cgaggcggtg ctcagcgtcg cagcgctcgt cctgttcgtc agctggccga tcgtcgctta
     2101 ttgcctgcgc gagccgtctg cggtcgaagc cgcggcgcgc aagctcacgg cggcgggcaa
     2161 gtcgttcggg ctgtcgttcc agcaatcgat gcgcgagccg acgttctgga tcctgaacat
     2221 cggtttcttt ctgctcggcg tcacggcgac gagcctcgtc acgcagcaag ttccgttgct
     2281 gcgcgatgca ggctggacgg ccgcggagac gacgcgcttg cagacgatct tcggcttcgg
     2341 cctgctgttc gcgcgcgtcg ccgtcggttt cgtcatcgat cacatcttcg cgccgcgcgt
     2401 gctgacgacg gtgtcgatcg gcggcgccgt cgcgtgcttg ttgtacgcgc tctatccgga
     2461 gtacggctat gtcagcgcga tcttgatcgg cttcttgctc ggagccgagt tcgacgtgct
     2521 cgccttcctc atcaagcgct attacggaaa cgtcgcttac ggccgcgtct atggcgtgat
     2581 cttcggcgtg ttctacttgg gctcggccgt cggcatctgg ggattgccca agcttcgcga
     2641 gctgtccgtg gatctctctt acgacaaacc cctctacgcc gcggccgcta tcctgctcgc
     2701 ttcggcagtg ctcatggcgt tcatgccgaa gtaccgatac gcggcgggac acaccgtggc
     2761 ggcgtcggcc ggcacgcccg cggcccaaca agcttgatcg agagaggagc agtcatgcgg
     2821 cccctgttgc tgaagacgtc tgcgcttctc gtcgccgtcg cgatctgcgg gggcgccgct
     2881 gcgcagcagc gcacgctgcg gcccgtcacc gacgagatgc tgcagaatcc ggacccggcc
     2941 gactggctgt cgtggcggcg cacgttgaat cactggggct atagcccgct gacggagatc
     3001 aacaaacgca acgtcgcgca gctgcgcctc gtctggacgc ggccgctcgc cgtcggcgtg
     3061 caagaaggca cgccgctcgt gcacgacggc gtcatgttct tcccgaatcc gaacgacatc
     3121 acgcaggcgc tcgacgccgc gaccggcgac ttgatctggg agtaccggcg gccggtgcag
     3181 gcggacaata acgactacat cccgttcccg tcgatcaacc gcaatctcgc gatctacggc
     3241 aacttgatcc tcgacaacgg cgccgacaac tacgcgtacg cgctcgacgc cgcgaccggc
     3301 gagctgaagt gggagacgca aatactcgac taccggcacg gcgcgcagca cagctcgggc
     3361 ccgatcgtcg cgaacggcaa agtcatctcg ggccgcagct gcgagccgga aggcggaccg
     3421 gaagcgtgcg tgctgacggc gttcgacgcg gcgacgggca acgagctctg gcgcacgcgc
     3481 acgatcccga agccggacga gccgggcggc gacacgtggg gcaacattcc ttacgaggag
     3541 cggcgccacg tcggcctctg gatggtgccg agcttcgatc ccgagcttag gcgcatctac
     3601 gtcggcacgt cggtcacgtc gccggcgccg aagttcatgc tcgcgggcaa cgacaagcag
     3661 tacctctatc acaacagcac gctcgcgatc gacgtcgaca cgggcaagat cgtttggtac
     3721 ttgcagcaca acgtcgacca ttgggatctc gatcatccgt tcgagcgaat cttgatcgac
     3781 acggaagtcg ctcccgatcc cgacgcggtc gcgtggatca acccgcgcgt ccgccgcggc
     3841 gagatgcaca aggtcatcac gggaattccg ggcaagacgg ggctcgtcta cacgctcgat
     3901 cgcgagacgg gccagttcct ctggtcgcgg ccgacggtcc agcagaacgt catcacgcgc
     3961 gtcaacacgg aatccggcga aggcgtcgtg aacacggaaa agctgttcac gggcccgaat
     4021 caagagattc tcgtgtgccc gagcctgacg ggcggcaaga actatcatgc cggcgcgtac
     4081 agcccggaca cgaagcttat ctacatgccg ttgcagaacg tctgcatgac cgcgacgtct
     4141 accgcgtcgc agccgagcct cgacgagctc tacgcggtac gcaccaagga tcaaatcgct
     4201 ccgggcgagc aaaacgtcgg cacgatcga
//
LOCUS       DASSNN010000025         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1518735, whole genome shotgun sequence.
ACCESSION   DASSNN010000025 DASSNN010000000
VERSION     DASSNN010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518735"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VFL84_00445"
     CDS             complement(<1..1175)
                     /locus_tag="VFL84_00445"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET7607116.1"
                     /translation="MKIGLAQINTRVGAIDANTRKVLDCAERARGMGCDLVLFPELTL
                     CGYPPEDLLLHRGLRARVEAAFALVRDEIRGIAAYVGYPEYEGDAIYNSAAMLRDGEV
                     LANHRKVALPNYAVFDEKRYFQPGQGATVADLGDYRFGLIICEDVWVPEPCRAPAAAG
                     AHAILVINGSPFHRTQQQAREQVLAARAEENSLPLLYVNMVGGQDELVFDGGSVVVDA
                     AGQVQFRAPLFEENVYVVELERRGGLAVPVAGTVTPVPPLAERVYNALVLGTRDYVEK
                     NGFAGVVLGLSGGIDSALTLAIAVDALGARRVHAVMMPSRYTSQMSRDDAHAQAKALG
                     VRYDVLSIEPMFETTLAALKDVFAGLPPDTTEENIQARCRGVLLMAISNKTGKMVLTT
                     "
     gene            complement(1172..2041)
                     /gene="sucD"
                     /locus_tag="VFL84_00450"
     CDS             complement(1172..2041)
                     /gene="sucD"
                     /locus_tag="VFL84_00450"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET7607117.1"
                     /translation="MSILVDKDTKVICQGLTGKQGTFHTQQCLEYGTKVVAGVTPGRG
                     GETHLNLPVFDTVADAKKATNATVSMIYVPAPFAADAILEAADAGIELVVCITEGVPV
                     LDMVKVKAVLKGTPTRLIGPNCPGIITPGACKIGIMPGSIHKPGSIGIVSRSGTLTYE
                     AVHQTTLRGLGQSTCVGIGGDPVRGMNFIDCIALFEKDPATKGIIMVGEIGGSDEEAA
                     AEYIAKSVTKPVVGYIAGVTAPPGKRMGHAGAIISGGKGTAKEKFAALEKAGVRTVRS
                     LADLGEAMATALK"
     gene            complement(2038..>2411)
                     /gene="sucC"
                     /locus_tag="VFL84_00455"
     CDS             complement(2038..>2411)
                     /gene="sucC"
                     /locus_tag="VFL84_00455"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006993812.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HET7607118.1"
                     /translation="GLAMATMDIIKLYGGEPANFLDVGGGATAERVTQAFKIILTNKN
                     VRAILVNIFGGIVRCDTIAEGVITAVKQVHVGVPVVVRLEGTNVEKGREMLDKSGLTV
                     VAATGLTDAASKVVKLAGAAR"
BASE COUNT          372 a          822 c          796 g          421 t
ORIGIN      
        1 gtcgtcaaca ccatcttgcc ggtcttgttc gagatcgcca tcagcagcac gccacggcac
       61 cgcgcctgga tgttctcttc cgtggtgtcg ggaggcagcc ccgcgaatac gtctttcagc
      121 gccgcgagcg tcgtctcgaa catcggctcg atcgacagca cgtcgtagcg cacgccgagc
      181 gctttcgctt gggcgtgtgc gtcgtcgcgg ctcatctgcg acgtgtaccg cgacggcatc
      241 atgacggcgt gcacacggcg cgcgccgagc gcgtcgacag cgatcgcgag cgtcaacgcg
      301 gagtcgatgc cgcccgacaa tccgagcacg acgccggcga agccgttctt ctcgacgtaa
      361 tcgcgcgtgc cgagcacgag cgcgttgtac acgcgctccg cgagcggcgg caccggcgtc
      421 acggtgcccg cgacgggaac ggccagcccg ccgcgccgct cgagctcgac gacgtacacg
      481 ttctcctcga acaacggcgc ccggaactgg acctggccag cggcgtcgac gacgaccgag
      541 ccgccgtcga acacgagctc gtcctggcca ccgaccatgt tgacgtagag gagcggcagt
      601 gaattctctt cggcccgtgc cgcgagcacc tgctcgcgcg cctgctgttg cgtgcggtga
      661 aacggcgagc cgttgatgac gagaatggcg tgcgcgcccg ccgcggccgg tgcgcggcag
      721 ggctccggca cccagacgtc ctcgcagatg atgaggccga acctgtagtc gccgaggtcg
      781 gcgacggtgg cgccctgccc cggctggaag tagcgcttct cgtcgaacac ggcgtaattc
      841 ggcagcgcga ccttgcgatg attggcgagg acttcgccgt cgcgcagcat cgcggcgctg
      901 ttgtagatcg cgtcgccttc gtactcggga tagccgacgt aggccgcgat gccgcggatc
      961 tcgtcgcgca cgagagcgaa cgcggcctcg acgcgagcgc gaaggccgcg atgcaggagc
     1021 aagtcctcgg gcggatagcc gcagagcgtg agctccggga acagcacgag atcgcagccc
     1081 atgccgcggg cgcgttctgc gcaatcgagt accttccggg tgttcgcgtc gatggcaccg
     1141 acgcgcgtgt tgatctgggc gagcccgatc ttcacttcag cgccgtagcc atcgcctcgc
     1201 cgagatccgc gagcgagcgg accgtgcgca caccggcttt ctcgagcgcc gcgaacttct
     1261 ccttcgcggt gcccttgccg ccggagatga tcgcgccggc gtggcccatg cgcttgccgg
     1321 gcggcgccgt cacgccggcg atgtagccga cgacgggctt cgtgacgctc ttcgcaatgt
     1381 actccgccgc ggcctcctcg tcggagccgc cgatctcgcc gaccatgatg atgcccttcg
     1441 tcgcggggtc tttctcgaac aacgcgatgc agtcgatgaa gttcatgccc cgcacggggt
     1501 cgccgccgat gccgacgcag gtgctttggc ccaagccgcg cagcgtcgtc tgatggacgg
     1561 cttcgtaggt cagcgttccc gagcgcgaca cgatgccgat cgagcccggc ttgtgaatcg
     1621 agcccggcat gatgccgatt ttgcacgcgc cgggcgtgat gataccgggg cagttcgggc
     1681 cgatcagccg cgtcggcgtg cccttcagca cggccttgac cttgaccatg tcgagcacgg
     1741 gcacgccttc cgtgatgcag acgacgagct cgatgccggc atcggctgct tcgagaatcg
     1801 cgtcggccgc gaacggcgcc ggcacgtaga tcatgctgac cgtggcattc gtcgccttct
     1861 tcgcgtcggc gaccgtgtcg aagaccggca gattcaaatg agtctcgccg ccgcggccgg
     1921 gcgtaacgcc ggcgacgacc ttcgtgccgt actcgaggca ctgctgcgta tggaacgtgc
     1981 cctgcttgcc ggtcaggccc tggcagatca ccttcgtgtc cttgtcgacc aaaatgctca
     2041 tcgggcggct ccggccagct tcacgacttt cgacgccgcg tcggtgagcc ccgtcgcggc
     2101 gacgaccgta aggccgctct tgtcgagcat ctcgcgtccc ttctcgacgt tcgttccctc
     2161 gaggcgcacg acgacgggca cgccgacgtg cacttgtttg acagcggtga tcacgccttc
     2221 cgcgatcgtg tcgcagcgca cgattccgcc gaagatgttg acgaggatcg ccctgacgtt
     2281 cttgttggtg aggatgatct tgaacgcctg cgtcacgcgc tcggccgtcg cgccgccgcc
     2341 gacgtcgaga aaatttgccg gctcgccgcc atacagcttg atgatgtcca tggtggccat
     2401 ggcgagaccg g
//
LOCUS       DASSNN010000026         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_510161, whole genome shotgun sequence.
ACCESSION   DASSNN010000026 DASSNN010000000
VERSION     DASSNN010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510161"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VFL84_00460"
     CDS             complement(<1..601)
                     /locus_tag="VFL84_00460"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004555217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET7607119.1"
                     /translation="MSEPLQTRVLVTGGAGFLGSHLCDRLVESGADVLCVDNFYTGRK
                     RNIAHLLPHASFELLRHDVTFPLYVEVDEIYNLACPASPIHYQHDPVQTTKTSVHGAI
                     NMLGLAKRLKARILQASTSEVYGDPALHPQREAYWGNVNPVGRRSCYDEGKRCAETLF
                     MDYRRQHGLTVKVARIFNTYGPRMHPEDGRVVSNFIVQAL"
     gene            complement(598..1542)
                     /locus_tag="VFL84_00465"
     CDS             complement(598..1542)
                     /locus_tag="VFL84_00465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7607120.1"
                     /translation="MRRLRVLTWHTHGSYLYYLTQAPHEFFVVSKPGRPPGYAGVCGH
                     LPWGANVHDLPASDVSRQRLDCVLFQDDAQYLTDQHELLSPEQRRLPKIYLEHDPPRA
                     NPTDELHPADDKSVLVVHVTPFNALMWRHERSPIRVIEHGVTEPEGVAYGGELPRGIV
                     VVNHIARRGRRLGFDLFVAARERVPLDLVGMAAEEAGGVGEVRHDALPEFASRYRFFF
                     NPIRYTSLGLAVIEAMMIGLPVVGLATTEMSTVVQNGATGYVDTSLDRLVARMQELVA
                     APAEARRLGAAARAYARERFGIGRFVADWNAAFLAVAV"
     gene            complement(1530..>2483)
                     /locus_tag="VFL84_00470"
     CDS             complement(1530..>2483)
                     /locus_tag="VFL84_00470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7607121.1"
                     /translation="LIGLPWARELVARLPGYVDEFLEFPGFPGLPERRCEPQAVLGFL
                     RATQDRRFDLALQLHGSGEFSNPFVALLGARRTAGYFREGRYCPDGATFLPWRDDEPE
                     VERGVRLLRALGVEPSGLELEAPVLPGDLRELAALAGERELPRRGYVCVHPGAQLESR
                     RWYPERFAIVADALAARGLRIVLTGTAGEAALAGRVARRMRAPVVDLVGRTTLGGLTA
                     LVDGARLLVTNDTGVRHIAAARGTPCVAVACGSDVRRWPASSPTQRLLHADMPCRPCT
                     HERCPIGHACAHAVESRDVLAAADELLVPALTIAGSSPCAA"
BASE COUNT          397 a          864 c          872 g          350 t
ORIGIN      
        1 gcagcgcctg cacgatgaag ttcgagacga cgcggccgtc ctcggggtgc atgcgtgggc
       61 cgtaggtatt gaagatgcgt gcaaccttga cggtaaggcc gtgctggcgg cgatagtcca
      121 tgaacagcgt ttcggcgcag cgtttgcctt cgtcgtagca cgagcggcgg ccgaccggat
      181 tcacgttgcc ccagtacgct tcgcgctgcg ggtgcaacgc cgggtcgccg taaacctcgc
      241 tcgtcgaggc ctgaaggatg cgagctttga gccgcttcgc gaggcccagc atgttgatcg
      301 cgccgtgcac gctcgttttc gtcgtctgca cgggatcgtg ctggtagtgg atcggcgacg
      361 ccgggcacgc gaggttgtaa atctcgtcga cctcgacgta gagcgggaac gtgacgtcgt
      421 gacgcagcag ctcgaagctc gcatgcggca acagatgcgc gatgttgcgc ttgcgtcccg
      481 tatagaagtt gtcgacgcag agcacgtcgg ccccgctctc gacgagccgg tcgcacagat
      541 gcgagccgag gaacccggcg ccgcccgtca cgagaacgcg cgtttgcagc ggctcgctca
      601 tacggccacc gcaaggaacg ccgcattcca gtctgcgacg aaacggccga tgccgaaccg
      661 ctcgcgcgcg tacgcgcgcg ccgcagcccc cagccgccgc gcttcggccg gcgccgcgac
      721 gagctcctgc atgcgcgcga cgagccggtc gagcgacgtg tcgacgtagc ccgtcgcgcc
      781 attttggacg acggtcgaca tctccgtggt cgcgagcccg acgacgggca ggccgatcat
      841 catcgcctcg atgacggcca atcctaagct cgtgtagcgg atcggattga agaagaaccg
      901 gtagcgcgac gcgaattcgg gcagcgcgtc gtggcgcacc tcgccgacgc cgccggcttc
      961 ctcggccgcc attccgacga ggtcgagcgg cacgcgctcg cgcgcggcaa cgaagaggtc
     1021 gaagccgagc cgccggccgc ggcgcgcgat gtgattgacg acgacgatgc cgcgcggcag
     1081 ctcgccgcca taagccacgc cctcgggctc cgtcacgccg tgctcgatga cgcgtatcgg
     1141 cgagcgctcg tggcgccaca tcagcgcatt gaacggcgtc acgtgcacga cgaggacgct
     1201 cttgtcgtcg gccgggtgca gctcgtcggt cggattcgcg cgcggtggat cgtgctcgag
     1261 gtagattttc ggcaaccgcc gctgctccgg gctcaagagc tcgtgctgat cggtcagata
     1321 ctgagcgtcg tcttggaaca gcacgcagtc gagcctctgg cggctgacgt cgctcgcggg
     1381 cagatcgtgg acgttcgcgc cccacggcag gtgcccgcaa acgccggcat aacccggggg
     1441 gcggccgggc ttcgagacga cgaagaactc gtgcggcgcc tgcgtcaggt agtacagata
     1501 gctgccgtgc gtgtgccagg tcagcactct caggcggcgc atggcgagct ccccgcgatc
     1561 gtaagcgccg gaacgaggag ctcgtcggcc gccgcgagca cgtcgcggct ctcgacggca
     1621 tgcgcgcagg cgtgcccgat cgggcagcgc tcgtgcgtgc acgggcggca cggcatgtcg
     1681 gcgtgcagca gccgctgcgt cgggctgctc gccggccagc gccgcacgtc gctgccgcac
     1741 gcgacggcga cgcacggcgt gccgcgcgcc gcggcgatgt gccgcacgcc cgtatcgttc
     1801 gtgacgagga gccgcgcgcc gtcgacgagc gcggtcagtc cgccgagcgt cgtgcggccg
     1861 accaagtcga cgacgggcgc gcgcatgcgc cgcgcgacgc ggcccgcgag cgccgcctcg
     1921 cccgccgtgc ccgtcaagac gatgcggagc ccgcgcgccg cgagcgcgtc cgccacgatc
     1981 gcgaagcgct cgggatacca gcggcgcgac tcgagctgtg cgccgggatg cacgcagacg
     2041 tagccgcggc gcggaagctc gcgctcgccg gcgagcgccg cgagctcgcg caagtcgccc
     2101 ggcaaaaccg gcgcttcgag ctcgagcccg ctcggctcga ccccgagcgc tcgaagcaac
     2161 cgcacgccgc gttcgacttc cggctcgtcg tcgcgccacg gcagaaacgt tgcgccgtcg
     2221 gggcagtagc gaccttcacg aaaatagccc gcggtgcgcc gcgctccgag cagcgcgacg
     2281 aacggattgc tgaactcgcc gctgccgtgc agctgcagcg ccaggtcgaa gcgcctgtcc
     2341 tgagtggccc gtagaaagcc gagcacggcc tgcggctcgc agcgccgctc gggcaacccc
     2401 ggaaatcccg ggaactcgag gaactcgtcg acgtaacccg gaagccgagc cacgagctcg
     2461 cgcgcccatg gcaagccgat caa
//
LOCUS       DASSNN010000027         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_885641, whole genome shotgun sequence.
ACCESSION   DASSNN010000027 DASSNN010000000
VERSION     DASSNN010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885641"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFL84_00475"
     CDS             complement(<1..740)
                     /locus_tag="VFL84_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET7607122.1"
                     /translation="MSTDSVTPPSRLAVASAFAIIYVIWGSTFLGILFAIQTLPPFLM
                     ASARFFIAGGLLYAWSRLVNGAAPPSLANWRATLAVGTLLLLGGNGLLVWSELRIPSG
                     VAALLVGTVPCFMVLLDWLRPGGTRPTGLVIAGLLLGLLGLVWLVGPDALMGGGRADL
                     LGAVVVVLGSFLWALGSVYSRHAATPQSPFLSTAMQMLGGGAALLLVSVVLGEPWAFD
                     ASGVSLRSVLGLLYLIVFGSIVAFSAYI"
     gene            complement(787..2133)
                     /locus_tag="VFL84_00480"
     CDS             complement(787..2133)
                     /locus_tag="VFL84_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607123.1"
                     /translation="MTRVATTRAVLASGALSVDALAAAAVWHARGVDSRTAPAAEAPI
                     GYPPTAPATGSMLRYETEYPTIPYTTGKRTDRVATLIRRIERGETELTFDSERGYLES
                     LLAQLEIDPSSQTLVYSQTSLQGKLIGPKTPRAIYFNDEVYVGYVQDGPIEIASMDSN
                     LGPVFYLLDQSAPNPKFTAELGRCMSCHDSYSLTGGGVPRFIVGSGYTGTLGQLVSHE
                     GWILVSDRTPLKSRWGGWYVTGMHGSQVHLGNIVIKTIYDFDKLEELRIGNRATLEGL
                     VDTKPYLTNGSDIVALLVLQHQADVQNLITRVSYDARSPADKREEPIEETVERLLRMM
                     LFVDAVEYTDPISGDAKFVQQFASRAVRDSQGRSLRDFDLTRRLFRYPLSYVIYSPAF
                     DALPADVRGMFYTRLNEVLTGADTSESFRHLSAADRSSVLEILRDTKPDAIVGAAN"
     gene            2204..2944
                     /gene="trxA"
                     /locus_tag="VFL84_00485"
     CDS             2204..2944
                     /gene="trxA"
                     /locus_tag="VFL84_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET7607124.1"
                     /translation="MDVSTATFEQEVLEASKTVPVVVDFWAPWCGPCRALGPTLDKVA
                     EAFGGRVKLVKINSDENPELSQAFNIRSIPNVMAFKDGRAVAQFTGAIPEAQVREFFG
                     QLVPSPAEETLRAAEAAFAAGNIDTAAELLAQVPRDIAVAERIAALERGISFAKAGAD
                     GPGEAELREALELDPLDHESRMKLASVLAGRRRFREAMDELLEVVRLGRHNQANAARE
                     QLLSLFALAASEPALVAEYRRKLASALH"
     gene            2947..>3057
                     /locus_tag="VFL84_00490"
     CDS             2947..>3057
                     /locus_tag="VFL84_00490"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HET7607125.1"
                     /translation="MQRTLITIGIAVLIAGLLWPWLSKLGLGRLPGDFRIQ"
BASE COUNT          552 a         1010 c         1025 g          470 t
ORIGIN      
        1 cagatgtagg cgctgaaggc gacgatcgag ccgaacacga tcagatacag cagtccgagc
       61 acggagcgca gcgatacccc gctcgcatcg aacgcccatg gctcgccgag cacgacgctg
      121 accaagagca gcgccgcgcc gccaccgagc atctgcatcg ccgtcgacaa gaatggggac
      181 tgcggcgttg ccgcgtgccg tgaatacacc gagcctagcg cccataagaa agaaccgaga
      241 acgacgacga ctgctccgag cagatccgca cggccgccgc ccatcagtgc gtcggggccg
      301 acaagccaaa cgaggcccag taacccgagc aataggcctg cgatgacgag gcctgtgggc
      361 cgcgttccgc ccggccggag ccagtcgagc agcaccatga agcaaggcac ggtgccgacc
      421 agcagcgccg cgacgccgga aggaatccgc agctccgacc agacgagcaa gccgttgccg
      481 ccgagcagaa gcagcgtgcc gacggctaaa gtggcgcgcc aattcgcgag gcttggaggc
      541 gcggcaccgt tcacgagacg cgaccacgcg tagagcagcc cgcccgcgat gaagaatcgc
      601 gcgctcgcca tcaggaaagg cggcaaagtt tgaatcgcga acaagatgcc gaggaacgtc
      661 gagccccaga tcacgtagat gatcgcgaat gcgctcgcca cagcgagccg ggacggaggt
      721 gtgacggagt ctgtgctcat ggggatggcg cggttgcggg ctcggacggc cgcgcaacgc
      781 aacgcttcag ttggctgcgc ctacgatggc gtcgggtttg gtgtcgcgaa ggatctcgag
      841 aacgctcgag cggtccgcgg cgctcaggtg ccggaagctt tcgctcgtgt cggcccccgt
      901 cagcacttcg ttcagacgcg tgtagaacat gccgcggacg tcggccggca gcgcgtcgaa
      961 cgccggcgaa tagatgacgt agctcagcgg atagcggaac agacgccgcg tcaaatcgaa
     1021 atcgcgcagc gaccggccct gcgagtcgcg cacggcgcgg ctcgcgaatt gctgcacgaa
     1081 tttcgcgtcg cccgagatcg ggtcggtgta ttcgaccgcg tcgacgaaca gcatcatgcg
     1141 cagcaaccgc tcgaccgtct cctcgatcgg ctcctcgcgt ttgtcggccg gcgagcgcgc
     1201 gtcgtagctc acgcgcgtga tcaggttctg cacgtcggcc tgatgctgca gcacgagcag
     1261 cgcgacgatg tcgcttccgt tcgtgaggta cggcttcgtg tcgaccaagc cctcgagcgt
     1321 ggcgcggttg ccgatacgca gctcctcgag cttgtcgaaa tcgtagatcg tcttgatgac
     1381 gatgttgccg agatggacct ggctgccgtg catgcccgtc acgtaccaac cgccccagcg
     1441 gctcttcagc ggcgtgcggt cggacacgag tatccagccc tcgtgcgaga cgagctggcc
     1501 gagcgtgccg gtgtagccgg agcccacgat gaagcgcggc acgccgccgc cggtcagcga
     1561 gtacgaatcg tggcagctca tgcagcggcc gagctcggcg gtgaacttag ggtttggcgc
     1621 gctctggtcg agcaaataaa agacgggccc gagattcgaa tccattgacg cgatctcgat
     1681 cggcccatct tgcacgtagc cgacgtagac ttcgtcgttg aagtaaatcg cgcgcggcgt
     1741 cttcgggccg atgagcttgc cctgcaagct cgtctgcgag tacacgagcg tttgcgacga
     1801 cggatcgatc tcgagctgcg cgagcagcga ttcgagatag ccgcgctcgc tgtcgaacgt
     1861 gagctcggtc tcgccgcgct cgatccggcg gatcagcgtc gcgacgcggt ccgtgcgctt
     1921 gcctgtcgta tacggaatcg tcggatattc ggtttcgtag cgcagcatcg agcccgtcgc
     1981 gggcgcggtc ggcgggtaac cgatcggagc ctccgcggcc ggtgccgtcc tcgaatcgac
     2041 gccgcgcgcg tgccacacgg cggcagccgc gagcgcgtcg acgctcaggg ctccggatgc
     2101 aagaacagca cgcgtagtgg cgactcgagt catgccgcgt agcttgccaa aacctcggca
     2161 ccgcggccac gggccgctcg agccgtacag tacaatggct cgcatggacg tcagcaccgc
     2221 aactttcgag caagaggttc tcgaagcttc gaagaccgtt cccgtcgtcg tcgatttctg
     2281 ggcgccgtgg tgcggcccgt gccgcgcgct cgggccgacg ctcgacaaag tcgccgaagc
     2341 attcggcggc cgcgtcaagc tcgtgaagat caactccgac gagaaccccg agctctcgca
     2401 agcattcaac atccgctcga tcccgaacgt catggcgttc aaggacggcc gcgccgtcgc
     2461 gcagttcacg ggcgccattc ccgaggcgca ggtccgcgag ttcttcggcc agctcgtgcc
     2521 gtcgcccgcc gaggagacct tgcgcgccgc cgaggccgca ttcgcggcgg gcaacatcga
     2581 tacggcggcc gagctgctcg cgcaggtgcc gcgcgacatc gccgtcgcgg agcggatcgc
     2641 ggcgctcgag cgaggcatct cgttcgcgaa agcgggcgcc gacgggcccg gcgaagccga
     2701 gctgcgcgag gcgctcgagc tcgatccgct ggaccacgag tcacgcatga agctcgccag
     2761 cgtgctcgcc ggccgccgcc gttttcgcga ggcgatggac gagctgctcg aggtcgtgcg
     2821 cctcgggcgg cacaaccaag cgaacgcggc gcgcgagcag ctgctgtcgc tgtttgcgct
     2881 tgcggcgtcg gagccggccc tcgtcgccga gtaccgccgc aagcttgcga gcgcactgca
     2941 ctagccatgc agcgcacgtt gatcacgatc ggaatcgccg tgctcatcgc gggcctgctc
     3001 tggccgtggc tgtcgaagct cgggctcggc cggttacccg gcgacttccg cattcaa
//
LOCUS       DASSNN010000028         5791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_6276, whole genome shotgun sequence.
ACCESSION   DASSNN010000028 DASSNN010000000
VERSION     DASSNN010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6276"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFL84_00495"
     CDS             <1..225
                     /locus_tag="VFL84_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607126.1"
                     /translation="AEVLWMLPYYIAVSAVYGGLTWAANSILPALVLHAAGDVVVLTR
                     SSLTGRPEWQIGPQRPLLVSESGVDRASTD"
     gene            365..2020
                     /locus_tag="VFL84_00500"
     CDS             365..2020
                     /locus_tag="VFL84_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HET7607127.1"
                     /translation="MASRHGKDGSKLDRRTFMSASVATGASLAFGGLTLRAAAQDRRL
                     TTVGGTAKTKYGQVRGLLKDGVQQFWCVPYGAPTGGANRFMPPQKPAAWSGVKDHFEI
                     TWAAPMDPNGEEPSPVVTALNRKTPQSEDCLTVNVFTPGLDNKARPVMVWMHGGGFSA
                     GSGNYLLYDGTNLAKKEDVVVVSVNHRLNIFGYLHLAGLGGEKWAGATNVGVQDLVAA
                     LQWVKDNIENFGGDPSRVTIFGQSGGGGKTTTVMAMPSARGLFHRSIAQSGSAIRGQT
                     ADDATEGAERFIAKLGIPKNQLERIQEIDWKAIQAAYYGEPRIARLGNGPVIDGTIIP
                     RHQWDPTAPSYSANVPFMAGSVENEDGWVGPPPYELPDDEMLQLFTNELANKNAADGQ
                     KLLALYKRRHPQTRNRMLWLMAESDDSRRWNAQLLCRLKHEQGAAPAYLYFFDWQSPV
                     HNNRMGAYHTLDIPFVFYNMDLGASMTGSSNARYQLGHVMSAAWAAFARTGDPNHADM
                     PTWPKFEPSNLPTMMFGPTVRVANDPNKEERLALAELRAKRPS"
     gene            2022..3047
                     /locus_tag="VFL84_00505"
     CDS             2022..3047
                     /locus_tag="VFL84_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607128.1"
                     /translation="MRARNVFSGLAVAAALAAVNPSLHAQNRESGATVEEQFTLPVPA
                     VEPLPRAFKTAEEHYNFLLERAHGGTKHTMQTIPVWDGLWGSGNNTMPSIFLEGGTLA
                     NAWRPGATKVKEGVLTPPYEKAFRERRAEIDQYGEQRYDRLTNCEYPGVPRWLWEPYI
                     KEFVNMPHQSWLMNDMMNETRRIYIGKEHVNIENKHSPLGDSIGFWDGDKLTIWTKWV
                     NPADYVRGMPLTSNQFEMVETWQERRAGDKRELVTQVTFYDPIGLVKPQQAVYVHESR
                     PDLEEAGVRIRMWECTTSSNSYKDAEGNTQFYLPGDPQYKDPRGFTDFPDIPGQSLNP
                     IFDSEQQ"
     gene            3049..3513
                     /locus_tag="VFL84_00510"
     CDS             3049..3513
                     /locus_tag="VFL84_00510"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7607129.1"
                     /translation="MNRAIVATAIVLSAASGTALAHHSFAMFDNSQHKLVEGVVTEWN
                     YNNPHSWLVIEAPGADGKMTTWSFEGAAIVHAARQGVNGKSYAKGEHVRVVMHPLRDG
                     RNAGALCFVIKEDGTFTRPNDGICNSDAMYARWQEKGWLAAGKHLDTHPTAD"
     gene            complement(3607..4461)
                     /locus_tag="VFL84_00515"
     CDS             complement(3607..4461)
                     /locus_tag="VFL84_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7607130.1"
                     /translation="MLELHRHSDARAFLTRAEPWLVAREIENGVVLQSARQARADDSR
                     YERPIYWATLEEDGRLVGCAYRTPPFKIGVTALPDAAIGPLVADLAATYPGPIGGFSG
                     PDPTVTILASAWVERRGGSWSVNTRGRLMSFSGGVTDDGAGKKGVLRLANATDTALAQ
                     SWGAAASIDSGITALDGNLCVELIRAKLLYFFVDDRARCMVGLLRETRDSVAVGIIYT
                     PATFRAQGYGTAALRALSRLLDERGIKNRYLWIDPKSDAAQALAAKLGCSFVYDSLDI
                     DCAEGRHA"
     gene            4531..5190
                     /locus_tag="VFL84_00520"
     CDS             4531..5190
                     /locus_tag="VFL84_00520"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET7607131.1"
                     /translation="MTLRIGETAPDFEADTTEGRIRFHDWIGNSWAVLFSHPKDFTPV
                     CTTELGTMARLKPEFDKRNVKIIGLSVDPVDSHSKWANDIKETQGYAPNYPLIGDPEL
                     KVSKLYNMLPAEAGATSQGRTAADNQTVRNVIVIGPDKKVKLILVYPMTTGRNFDEVL
                     RVVDSLQLTAKHRVATPANWKQGDDVIIAGSVTDDEAKKIYPTGWKAPKPYLRIVPQP
                     R"
     gene            5308..>5791
                     /locus_tag="VFL84_00525"
     CDS             5308..>5791
                     /locus_tag="VFL84_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HET7607132.1"
                     /translation="MRRRQIAGALEEWHASAFEHALDQFDAAARRLKLTENQIAMTKL
                     PRRVTEVLLPVRMDDGEIRNFRAFRVQHNMARGPAKGGIRFHQDVTVDEIKALAFWMT
                     YKCAVVGIPMGGAKGGVIVDPRTLSTGERERLARRYMAELIDLFGPDSDVPGPDVNTG
                     P"
BASE COUNT         1096 a         1879 c         1947 g          869 t
ORIGIN      
        1 gccgaggtct tgtggatgct gccgtactac atcgccgtct cggccgtgta cggcggattg
       61 acctgggcag cgaactcgat cttgcccgcg ctcgtcttgc acgcggccgg cgacgtcgtc
      121 gtgctgacgc ggtcgtcgct caccggccgg cccgaatggc agatcggccc gcagcggccg
      181 ctgctcgtat cggagagcgg cgtcgaccgc gcgagtaccg attgactcgc gtggctgcgg
      241 cgcccaacgc aggacccgtc gattggcttt gttcagccga accgggcggt agggcgcgtt
      301 tcccaaggct tggcgtatag tcggcgaagc caaccgctca atcagaaatt cgggggaacg
      361 aacgatggct tcacggcacg gcaaggacgg ctccaagctc gaccgcagga ctttcatgag
      421 cgcgagcgtc gctacgggcg cgagcctcgc tttcggcggg ctcacgctgc gcgccgcggc
      481 gcaagatcgg cgcctcacga cggtcggcgg caccgcgaag acgaaatacg gtcaggtgcg
      541 cggcttgctc aaggacggtg tgcagcagtt ctggtgcgtg ccgtacggcg cgccgacggg
      601 gggcgcgaac cgcttcatgc cgccgcagaa gccggccgca tggtcagggg tcaaggatca
      661 cttcgaaatc acctgggccg ctccgatgga tccgaacggc gaggagccgt cgcccgtcgt
      721 gacggcgctg aaccgcaaga cgccgcagag cgaggactgc ctcaccgtca acgtcttcac
      781 gccgggcctc gacaacaagg cgcggcccgt catggtctgg atgcacggcg gcggcttctc
      841 ggcgggctcc ggcaactact tgctctacga cggcacgaac ctcgcgaaga aggaggacgt
      901 cgtcgtcgtc tccgtcaatc accgcctcaa catcttcggc tacctgcacc tcgcggggct
      961 cggcggcgag aagtgggccg gcgcgacgaa cgtcggcgtg caagacctcg tcgccgcgct
     1021 gcagtgggtc aaggacaaca tcgagaattt cggcggcgac ccgagccgcg tcacgatctt
     1081 cggccagtcc ggcggcggcg gtaagacgac gaccgtcatg gcgatgccgt cggcgcgcgg
     1141 cttgttccat cgctcgatcg cgcagagcgg ctcggcgatc cgcggccaga cggccgacga
     1201 cgcgaccgag ggcgccgagc gcttcatcgc gaagctcggc attccgaaga atcagctcga
     1261 gcgcattcaa gagatcgatt ggaaggcaat ccaagccgcg tactacggcg agccgcgcat
     1321 cgcgcggctc ggcaacgggc ccgtcatcga cggcacgatc atcccgcgcc atcagtggga
     1381 tccgacggcg ccgtcgtact ccgcgaacgt gccgttcatg gcgggctccg tcgagaacga
     1441 ggacggttgg gtcggcccgc cgccgtacga gctgcccgac gacgagatgc tgcagctctt
     1501 cacgaacgag ctcgcgaaca aaaatgcggc cgacggccag aagctgctcg cgctgtacaa
     1561 gcgccgccat ccgcagacgc gcaaccgcat gctctggctc atggccgagt cggacgactc
     1621 gcggcgctgg aacgcacagt tgctctgccg cttgaagcac gagcagggcg cggcgccggc
     1681 ctatctctat ttcttcgact ggcaatcgcc cgtgcacaac aaccgcatgg gcgcgtatca
     1741 cacgctcgac attccgttcg tgttctacaa catggacctc ggcgcctcga tgacgggctc
     1801 ttcgaacgcg cgctaccagc tcggccacgt catgagcgcg gcatgggccg cgttcgcgcg
     1861 cacgggcgac ccgaaccacg ccgacatgcc gacctggccc aagttcgagc cgagcaactt
     1921 gccgacgatg atgttcggcc cgacggtgcg cgtcgcgaac gatccgaaca aggaagagcg
     1981 gctcgcgctc gcggagctgc gcgcgaagcg gccgtcgtaa catgcgcgcc cgcaacgtct
     2041 tctcgggatt ggccgtcgcg gccgcgttag cggcggtcaa tccctcgctg cacgcgcaga
     2101 accgcgagag cggcgccacg gtcgaggagc agttcacgct gccggtgccg gccgtcgagc
     2161 cgttgccgcg cgcgttcaag acggccgagg agcactacaa cttcctgctc gagcgcgcgc
     2221 acggcggcac gaagcacacg atgcagacga tccccgtgtg ggacggcctc tggggcagcg
     2281 gtaacaacac gatgccgtcg attttcttgg agggcggcac gctcgcgaac gcgtggcggc
     2341 ccggcgcgac gaaggtcaag gagggcgtgc tgacgccgcc gtacgagaaa gcgtttcgcg
     2401 agcgccgcgc cgagatcgac cagtacggcg agcagcgcta cgaccgcctc acgaactgcg
     2461 agtatcccgg cgtgccgcgc tggctctggg agccgtacat caaagagttc gtgaacatgc
     2521 cgcatcagtc gtggctcatg aacgacatga tgaacgagac gcggcgcatc tatatcggca
     2581 aagagcacgt caacatcgag aacaagcact cgccgctcgg cgacagcatc ggcttttggg
     2641 acggcgacaa gctcactatc tggacgaagt gggtcaaccc cgccgactac gtgcgcggca
     2701 tgccgctcac gagcaatcag ttcgagatgg tcgagacgtg gcaagaacgg cgcgccggcg
     2761 acaagcgcga gctcgtcacg caggtcacgt tctacgatcc gatcgggctc gtgaaaccgc
     2821 agcaagcggt gtacgtgcac gagtcgcggc ccgatctcga ggaagccggc gtgcgcatcc
     2881 gcatgtggga gtgcacgacg agcagcaact cgtacaagga cgccgaaggc aacacgcagt
     2941 tctatctgcc cggcgatccg cagtacaaag acccgcgcgg gttcacggat tttccggata
     3001 tcccgggtca atcgttgaac ccgatcttcg attcggagca gcagtgaaat gaaccgagcg
     3061 atcgttgcaa ccgccattgt gctgagtgcg gcttcgggta ctgcgctagc gcatcattcg
     3121 ttcgcgatgt tcgacaactc gcagcacaag ctcgtcgaag gtgtcgtcac ggagtggaac
     3181 tacaacaacc cgcatagctg gctcgtcatc gaggcgcccg gcgccgacgg caagatgacg
     3241 acgtggagct tcgaaggcgc cgcgatcgtg cacgccgcgc gccaaggcgt gaatggcaag
     3301 agctacgcga aaggcgagca cgtgcgcgtc gtcatgcacc ccttgcgcga cggccgcaac
     3361 gccggcgcgc tgtgcttcgt catcaaagag gacggcacgt tcacgcggcc gaacgacggc
     3421 atctgcaact cggatgcgat gtacgcgcgc tggcaggaga agggctggct cgcggcgggt
     3481 aagcatctcg acacgcaccc gacggccgac tgacctgcaa tgagctgcag gcgggtgtaa
     3541 gtttcgcctt gcgccgctag cggcgagcgg caaccaacgc cagccgcgat gtcgaagctc
     3601 gcaatgtcac gcgtgccgcc cttcggcgca gtcgatatcg agtgagtcgt agacgaagct
     3661 gcaaccgagc ttcgcggcga gcgcctgcgc ggcgtcgctc ttcgggtcga tccacaagta
     3721 gcggttcttg atgccgcgct cgtcgagcag gcggctcaag gcgcgcagcg cggccgttcc
     3781 gtaaccttgc gcacggaacg tcgcgggcgt gtagatgatg ccgacggcga cggagtcgcg
     3841 cgtctcgcgc aagaggccga ccatgcagcg cgcgcgatcg tcgacgaaga agtagagcaa
     3901 tttggcgcgg atcagctcga cgcacagatt gccgtcgagc gccgtgatgc cgctgtcgat
     3961 cgacgccgcg gcgccccagc tctgcgcgag cgccgtatcg gtcgcgttgg cgagccgcag
     4021 cacgcctttc ttgccggcgc cgtcgtcggt cacgccgccc gagaacgaca tgaggcgccc
     4081 gcgcgtgttg acggaccagg agccgccgcg ccgctcgacc cacgcgctcg cgagaatggt
     4141 gaccgtcgga tcggggccgc tgaagccgcc gatcgggccg gggtaggtgg ccgcgaggtc
     4201 cgcgacgagc ggtccgatcg ccgcgtccgg caatgccgtg acgcctatct tgaaaggcgg
     4261 cgtgcggtag gcgcagccga cgagccggcc gtcctcttcc agcgtggccc aatagatcgg
     4321 ccgctcgtag cgggaatcgt cggcgcgcgc ctggcgcgcg ctctgcaaaa cgacgccgtt
     4381 ctcgatttcc cgcgccacga gccaaggctc ggcgcgcgtg aggaaggcgc gcgcgtctga
     4441 gtggcggtga agctcgagca tggggtgctc actataccgc gcgaagggct agaatcattc
     4501 cctcaaccgc acgtgaaacg gagaacgacc atgaccctgc gcatcggcga gacggctccc
     4561 gacttcgagg ccgacaccac tgaaggccgc atccgcttcc acgattggat aggcaactcg
     4621 tgggcggtct tgttctcgca tccgaaagac ttcacgccgg tctgcacgac ggagctcggc
     4681 acgatggcgc ggctgaagcc cgagttcgac aagcgcaacg tgaaaatcat cggcttgagc
     4741 gtcgatcccg tcgacagcca ttcgaagtgg gccaacgaca tcaaggagac gcagggctac
     4801 gcgccgaact atccgctgat cggcgatccc gagctcaagg tgtcgaagct ctacaacatg
     4861 ctgccggccg aggcgggcgc aacctcgcag ggccgcacgg cggccgataa ccagacggtg
     4921 cgcaacgtca tcgtcatcgg gccggacaag aaggtgaagc tgatcctcgt gtatccgatg
     4981 acgacagggc gcaacttcga cgaggtgctg cgagtcgtcg attcgctgca gctgacggca
     5041 aagcatcgcg tcgcgacgcc ggcgaactgg aagcaaggcg acgacgtcat catcgcgggc
     5101 tccgtcaccg acgacgaggc gaaaaagatc tatccgacgg gttggaaggc gccaaagccg
     5161 tatttgagga tcgttccgca gccgcgctga agcgccgcta caagggcaac ggacaataac
     5221 aatgacgaac tgcaagctct gtcttcgcga atgccgcgtg aaaaattagc gaaggcgcag
     5281 cgcaaacaga tcgagcgcaa ggcggccgtg cggcgacgcc agatcgcggg cgcgctcgag
     5341 gagtggcacg cgtcggcgtt cgagcatgcg ctcgatcaat tcgacgcggc ggcgcggcgg
     5401 ctaaagctca ccgagaatca gatcgcgatg acgaagctgc cgcggcgcgt gaccgaggtg
     5461 ctgctcccgg tgcgcatgga cgacggcgag atccgcaact tccgcgcgtt tcgcgtgcag
     5521 cacaacatgg cgcgcggccc ggcgaagggc ggcatccgct tccatcaaga cgtcacggtc
     5581 gacgagatca aggcgctcgc gttctggatg acgtacaagt gtgccgtcgt cggcattccg
     5641 atgggcggcg cgaaaggcgg cgtcatcgtc gatccgcgca cgctgtcgac gggcgagcgc
     5701 gagcgtctgg cgcgccgcta catggcggag ctgatcgacc tcttcgggcc cgacagcgac
     5761 gtgccgggcc cggacgtcaa caccgggccg c
//
LOCUS       DASSNN010000029         5964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_886061, whole genome shotgun sequence.
ACCESSION   DASSNN010000029 DASSNN010000000
VERSION     DASSNN010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886061"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFL84_00530"
     CDS             complement(<1..473)
                     /locus_tag="VFL84_00530"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET7607133.1"
                     /translation="MNDVSSAAPRAAYSGFWRRFAAWAIDCLVISVGSVVLRIVAPLV
                     GIGGDTRFWLVFVAAYFFYCTLLESSPWQASIGKRALGIKVTNENSARIGFARAAVRF
                     AAKLLSVLTLCLGYLLIVVTKRGQALHDLIAGTLVAHDAESSRPAWVVSAAACAP"
     gene            complement(483..1244)
                     /locus_tag="VFL84_00535"
     CDS             complement(483..1244)
                     /locus_tag="VFL84_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7607134.1"
                     /translation="MELRGKTILITGAGRGLGAAMAKRLAARGAGLALLDLDDRGLVA
                     VRDACRALGVRAETYSANVAVEQEVVGTFDRVVADFGRLDGSIANAGILRDGLLVKAK
                     DGEVTGKLSLEQWQSVIDVNLTGVFLCGREAAERMIRCGNGGCIINISSLSRAGTFGQ
                     SNYSAAKAGVAALTVVWAKELARHEIRVNAIAPGFIKTEMVAGMKPEMLEKMAAGIPV
                     KRLGEPDEIAAAAEFIFTNDYLNGRILEIDGGQRL"
     gene            1434..1820
                     /locus_tag="VFL84_00540"
     CDS             1434..1820
                     /locus_tag="VFL84_00540"
                     /inference="COORDINATES: protein motif:HMM:NF015180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HET7607135.1"
                     /translation="MLLSKYVDRVAIALSTICIVHCLAMPFLIALLPVAALTFGGDGH
                     FHALMLWFVVPTSIIGLGLGVRVHRRFAIVALGALAVAVLATAALWGHAAWDPTVEVF
                     VNVAASIALAAAHWRNFREVRRLHHH"
     gene            complement(1814..3274)
                     /locus_tag="VFL84_00545"
     CDS             complement(1814..3274)
                     /locus_tag="VFL84_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7607136.1"
                     /translation="MTAAPLVTLDHVDVDLAGSPVLRDVTWRLVPGEHWGVVGANGSG
                     KSTLLGLIAGTVWPAPERGLRRYDFGFGEETDAVRARDEIVLVGHELQDRYARRDWNF
                     TALEVVLSGIYRTDVPRRKPAADQRERALDVLRRLGVERLAERRFLELSRGEQRRVLI
                     ARGVAFGPSVLLLDEPASGLDARARVELDAMLELVARECTLVCAAHRPEELPSSIARY
                     LLIEGGRVEPVELDRSTRGPAQLAAGAAPAIAAPRAPAASTPLIRLERADVWLAGRHV
                     LRDVSWRLMPGEQWLVTGANGSGKSTFLRMLHGQLRPALGGSVSWPALGFPRDVWELR
                     KQVAWVSAELQAAYRYPSTVRACIASGFTSSVGATRRPTAEESRRIEELLDELELVPL
                     ADRLLSTLSYGQARRALLGRLLANRPRVLLLDEPWEGLDAPMAELLNATLERVAADGT
                     QLVCASHLSTHRSHFTHELTLDAGAVVHAGATRALQ"
     gene            3405..4670
                     /locus_tag="VFL84_00550"
     CDS             3405..4670
                     /locus_tag="VFL84_00550"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET7607137.1"
                     /translation="MRRALPWLAGLVCAFAASAGGIYESVPIDKDEWRLIEESKDLEG
                     WFGAQSLLYTEPKALELVRRVGHEIRPQPTDDYIEYEFFVLRDPSPNAFALPNGHVYV
                     HTGMLARLDDEDELAALLAHEINHVAGHHGIIEHRATRKKTIAGMVIGSLGPWGGVIS
                     LGLQTSVYGYSRELEQEADDRAIEVLRASRYDPHALPDVLDILGRDYEGLSPRTPTVW
                     STHPEVRARAATSRALVAAVPPRERHGDGFEAAVMEMRAMTIEDYVQDDFPHTAVALA
                     ESLVARYPSDLELLNLLGDAWQGMGAQGPLDLDSLSDSDKRHNLRDRARKTRAQRLAE
                     ALQTEEGRAAYAANLQRAEETYQRAIAADATFAPAYRGLGEVYEQQARTRDAAEAYLT
                     YIRQAPAAPDRAIVMGRLRLITTQLKAGQ"
     gene            4672..5433
                     /locus_tag="VFL84_00555"
     CDS             4672..5433
                     /locus_tag="VFL84_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607138.1"
                     /translation="MSISIRAVVTLAAALSCSVAAFAAERGFTNEFLSGATRPKSIAL
                     LPAEATITRQQVVETTGLIDESVVFGAQFNADVAKVLADKGYEVQIVDADRINADREL
                     QEYVVDAKRAYDEMIARYRPKKLPDRIYNAGDSARLLGAHLGVDAIAFSKLSLTITPA
                     GKAIVSALIGGTTSGANSMIGIVDGDTGDLEVVQIAIAIVTPGEKTPDEIAAYVAGLA
                     VNNTKRLPGADPNARVDVAVSDDEVLDEVESLIKE"
     gene            5513..>5964
                     /locus_tag="VFL84_00560"
     CDS             5513..>5964
                     /locus_tag="VFL84_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7607139.1"
                     /translation="MTNVLPTVADVVDAEARLAGWVVETPVLESQAINARVGARVLLK
                     AECLQHTGSFKIRGAMNRLLQLSADERAAGVVAFSSGNHAQAVALAARWLKVPATIVM
                     PADAPKIKVERTRENGAEIVLYDREREDREAIAAGIAERRGAAIVPPF"
BASE COUNT          982 a         2060 c         2047 g          875 t
ORIGIN      
        1 aacggcgcgc aggccgctgc cgagacgacc cacgcgggcc gcgacgactc cgcatcgtgc
       61 gcgacgagcg ttcccgcgat caggtcgtgc agcgcctgcc cgcgcttcgt cacgacgatg
      121 agcagatacc cgaggcagag cgtcagcacc gacagcagct tcgccgcgaa gcgcacggcc
      181 gcgcgcgcga acccgatgcg cgcgctgttc tcgttcgtga ccttgatccc gagcgcgcgc
      241 ttaccgatgc tggcctgcca cggcgagctc tcgagcagcg tgcagtagaa gaagtacgcc
      301 gcgacgaaga cgagccagaa gcgcgtgtcg ccgccgatgc cgaccagcgg cgcgacgatg
      361 cgcagcacga cgctgccgac cgagatcacg aggcagtcga tcgcccaagc ggcgaatcgg
      421 cgccagaagc ccgaatacgc cgcgcgcgga gccgcgctcg acacgtcgtt cacgtcaatc
      481 tcctagagtc gctggccgcc gtcgatctcg aggatgcgtc cgttcagata atcgttcgtg
      541 aagatgaatt ccgccgccgc cgcgatctcg tcgggctcgc cgagccgctt gaccggaatt
      601 ccagccgcca tcttctcgag catctcgggc ttcatgccgg ccaccatctc agtcttgatg
      661 aagcccggcg cgattgcatt gacgcggatc tcatgccgcg cgagctcctt ggcccagacg
      721 accgtcagcg ccgctacgcc ggccttggcg gccgagtaat tgctctgccc gaaagtgccg
      781 gcgcgcgaca agctcgagat gttgatgatg cagccgccgt tgccgcagcg aatcatgcgc
      841 tcggccgctt cgcggccgca caagaaaact ccggtcaagt tgacgtcgat gacggactgc
      901 cattgctcga ggctcagctt gccggtcacc tcgccgtcct tcgctttgac gagcaagccg
      961 tcgcgcagga tgccggcatt cgcgatgctg ccgtcgagcc gcccgaagtc ggcgacgacg
     1021 cgatcgaaag tgccgacgac ctcttgctcg acggcgacgt tcgcggagta ggtctcggcg
     1081 cggactccga gcgctcggca agcgtcgcgg accgcgacga ggccgcggtc gtcgagatcg
     1141 aggagcgcga ggccggcgcc gcgcgcggcg agccgtttgg ccatcgcggc gccgagcccg
     1201 cggccggcgc ccgtgatcag gatggttttg ccgcgaagtt ccatgcgtca ccccgagcga
     1261 cttgctcgcg cacttcgaga acgatagtag cgtgtggagc cgcaaaatgc gtcctgcatt
     1321 tacggcactc ccgccatcca cggcggtcgg gcgcagccgc cgaaaacagg ccgagggccg
     1381 cgaaactacg gtatgctcgc gggccgaccg aagaatctgc tctcgagctt ccgatgctgc
     1441 tttcgaaata tgtcgaccga gtggcgatcg cgctctcgac gatctgcatc gtccactgcc
     1501 tggcgatgcc gttcctcatc gcgctattgc ccgtcgccgc gctcacattc ggcggcgacg
     1561 gccattttca tgcgttgatg ctctggttcg tggtgccgac gagcatcatc gggctcggcc
     1621 tcggcgtgcg cgtgcatcgg cgtttcgcga tcgtcgcgct cggcgcgctc gcggtggcgg
     1681 tgctcgcgac cgcggcgctc tggggtcacg ccgcatggga cccgaccgtc gaggtcttcg
     1741 tcaacgtcgc ggccagcatc gcgctcgcgg cggcgcactg gcgcaacttc cgcgaagttc
     1801 gccgcctgca tcatcactga agcgcgcgcg tcgccccggc gtgcacgacc gcgccggcgt
     1861 cgagcgtaag ctcatgcgtg aagtgcgagc ggtgcgtcga gagatgcgac gcgcacacga
     1921 gctgcgttcc atcggccgcc actctttcga gcgtggcgtt caacagctcg gccatcgggg
     1981 cgtcgagccc ttcccacggc tcgtcgagca acagcacacg cggtcgattc gcgagcagcc
     2041 ggccgagcag cgcgcgccgc gcctgcccgt acgacagcgt cgacaacagc cggtccgcga
     2101 gcggcacgag ctcgagctcg tcgagcaact cctcgatgcg ccgcgactct tcggccgtgg
     2161 gccggcgcgt tgcgccgacg ctcgacgtga accccgaggc gatgcacgcg cgcaccgtcg
     2221 acgggtagcg gtatgccgct tgcagctcgg ccgacaccca ggccacttgc ttgcgcagct
     2281 cccagacgtc gcgcggaaac ccaagcgcgg gccacgacac gctgccgccg agcgcgggcc
     2341 gcagctgacc gtgcagcatg cgcaggaagg tgctcttgcc cgagccgttc gcgccggtca
     2401 cgagccactg ctcgccgggc atgagccgcc aactgacgtc gcgcagcacg tgccggccgg
     2461 cgagccagac gtccgcgcgc tcgagccgga tcagcggcgt cgaggcggcc ggagctcggg
     2521 gcgccgcgat cgcaggagcc gcgcccgccg cgagctgcgc tgggccgcgc gtgcttcggt
     2581 cgagttcgac cggctcgacg cggccgcctt cgatgagaag atagcgcgcg atcgacgagg
     2641 gcaactcttc gggccgatgc gcggcgcaca cgagcgtgca ttcgcgcgcg acgagctcga
     2701 gcatcgcgtc gagctcgacg cgcgcgcgag cgtcgagccc gctcgcgggc tcgtcgagca
     2761 gcaacaccga cggcccgaac gcgacgccgc gcgcgatcag cacccgccgc tgctcgccgc
     2821 gcgatagctc gaggaagcgg cgttccgcga gccgctcgac gccgaggcgg cgcaacacgt
     2881 cgagcgcgcg ctcgcgttgg tcggccgcgg gcttgcggcg cgggacgtcg gtgcgataga
     2941 tgccggagag cacgacctcg agcgccgtga agttccagtc cctgcgcgcg tatcgatcct
     3001 gcagctcgtg accgacgagc acgatctcgt cgcgcgcgcg cacggcgtcg gtctcttcgc
     3061 cgaagccgaa gtcgtagcgg cggagcccgc gttcaggcgc gggccagacg gtgcccgcga
     3121 tcaggccgag cagcgtgctc ttgccgctgc cgttcgcgcc gacgacgccc caatgctcgc
     3181 cgggcacgag tcgccacgtg acgtcgcgca gcacgggcga gccggcgaga tcgacgtcga
     3241 catgatcgag cgtgacgaga ggtgcggcgg tcatagggcc gcgcgagttt accaagtgcc
     3301 gtccgcggtg tggcgcgcgt cacagtccgg cgagcgctct gcggcgggga gcggcggccc
     3361 gcgctacgat tcgggcctcg tccaatggcc acggacttcc cttcatgcga cgagcgctcc
     3421 catggcttgc cggtctcgtt tgcgcgttcg ccgcgtccgc cggcggcatc tacgaatccg
     3481 ttccgatcga caaagacgaa tggcggctca tcgaggagtc gaaggacctc gaaggctggt
     3541 tcggcgcgca gtcgctgctc tacacggagc ccaaagcgct cgagctcgtg cgccgcgtcg
     3601 ggcacgagat ccgtccgcaa ccgacggacg actacatcga gtacgagttc ttcgtgctgc
     3661 gcgatccgtc gccgaacgct ttcgcactgc cgaacggcca cgtctacgtg cacaccggca
     3721 tgctcgcgcg gctcgacgac gaggacgagc tcgcggcgct gctcgcgcac gagatcaatc
     3781 acgtcgcggg ccatcacggc atcatcgagc accgcgcgac gcgcaagaag acgatcgcgg
     3841 gaatggtgat cggcagcctc gggccatggg gcggcgtgat ctcgctcggc ctgcagacgt
     3901 cggtctacgg ctatagccgc gagctcgagc aggaggcgga cgaccgcgcg atcgaagtgc
     3961 tgcgcgcgag ccgctacgac ccgcacgcgc tgccggacgt gctcgatatc ttgggccgcg
     4021 actacgaggg gttgagcccg cgtactccga ccgtctggag cacgcacccc gaggtccgcg
     4081 cgcgcgccgc gacgagccgc gcgctcgtcg ccgcagtgcc gccccgcgag cgccacggcg
     4141 acgggttcga agccgccgtc atggaaatgc gcgcgatgac gatcgaggac tacgtccagg
     4201 acgattttcc gcacacggcc gtcgcgctcg ccgagtcgct cgtcgcgcgt tatccgagcg
     4261 acctcgagct ccttaatctg ctcggcgatg cgtggcaagg catgggcgcg caagggccgt
     4321 tggatctcga ttcgctgtcc gattccgaca agcggcacaa cttgcgcgac cgcgctcgca
     4381 agacgcgcgc gcagcgcttg gccgaggcgc tgcaaaccga ggaaggccgt gccgcgtacg
     4441 cggcgaattt gcagcgcgcc gaggaaacgt atcagcgcgc gatcgcggcc gatgcgacgt
     4501 tcgcgcccgc ttaccgcggc ctcggcgaag tctacgagca gcaagcgcgg actcgcgacg
     4561 ccgcagaggc ctatctcacc tacatcaggc aggcgccggc cgcgcccgat cgcgcgatcg
     4621 tgatgggccg gctacgactc attaccacgc aattgaaggc agggcagtga catgagcatc
     4681 agcattcgag cagtcgtgac gttggcggcc gcgctctcgt gcagcgtggc ggcgttcgcc
     4741 gcggaacgag ggttcacgaa cgagttcttg agcggcgcga cgcggccgaa gtcgatcgcg
     4801 ctgctgccgg ccgaggcgac gatcacgaga cagcaagtcg tcgagaccac gggtctcatc
     4861 gacgagagcg tcgtgttcgg cgcgcagttc aacgccgacg tcgcgaaggt tctcgccgac
     4921 aagggctacg aggtgcaaat cgtcgacgct gaccgcatca acgccgatcg agagctgcag
     4981 gagtacgtcg tcgacgcgaa gcgcgcgtac gacgagatga tcgcgcggta ccggccgaag
     5041 aagctgcccg atcgcatcta caacgcgggc gactccgcgc ggctgctcgg cgctcacttg
     5101 ggcgtcgacg caatcgcgtt ctcgaagctc agcctcacga tcacgcccgc gggcaaagcc
     5161 atcgtgtccg cgttgatcgg cggcacgacc tcgggcgcca attcgatgat cggcatcgtc
     5221 gacggcgaca cgggcgatct cgaggtcgtg cagatcgcga tcgcgatcgt tacgccgggc
     5281 gagaagacac cggacgagat agccgcgtac gtggcgggcc tcgccgtgaa caacacgaag
     5341 cgcctgccgg gggccgaccc gaacgcgcgc gtcgacgtcg ccgtcagcga cgacgaagtg
     5401 ctcgacgagg tcgagtcgct gatcaaagaa tagtcgcgaa ccgggaacga tctgccgtcg
     5461 gtggggcgcc gctgacattc ttttgcgcgg cggcgctaga ctagcgcccg ccatgacgaa
     5521 tgtgctgccc acagtcgcgg acgtcgtcga cgcagaggcg cgcttggccg gctgggtcgt
     5581 cgagacgccg gtgctcgagt cgcaggccat caacgcgcgc gtcggcgcgc gcgtcctgct
     5641 caaggccgaa tgcctgcagc acacgggcag cttcaagatc cgcggcgcga tgaatcggct
     5701 gctgcagctc agtgccgacg agcgtgccgc cggcgtcgtc gccttctcgt cgggcaatca
     5761 cgcgcaggca gtggcgctgg cggcgcgctg gctcaaagtg ccggccacga tcgtgatgcc
     5821 ggccgacgcg ccgaaaataa aggtcgaacg aacgcgcgag aacggtgccg agatcgtgct
     5881 ctacgatcgc gagcgcgagg accgcgaggc catcgcggcc ggcatcgcgg agcggcgcgg
     5941 agcggcgatc gtgccgccgt tcga
//
LOCUS       DASSNN010000030         3516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1015320, whole genome shotgun sequence.
ACCESSION   DASSNN010000030 DASSNN010000000
VERSION     DASSNN010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015320"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFL84_00565"
     CDS             <1..223
                     /locus_tag="VFL84_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607140.1"
                     /translation="GLAGSLMPRGETGNAMGIVSLGAGLFGYFGPQMLGILRDATGSF
                     AAGFYMIAACDLMTLALIVVLYRLTRSAR"
     gene            236..1054
                     /locus_tag="VFL84_00570"
     CDS             236..1054
                     /locus_tag="VFL84_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7607141.1"
                     /translation="MNRSVFVFIVAWLGVTATAAQSAPATGDQHRTYVFAPTGAEMPY
                     RVYVPTSWDGKRSLPILLFLHGAGANESTYLDIADGLLRKLAEQHGYIVVSPLGFTPL
                     GAYGNPLRLPAVFGESEAAAAQRAAVTPQRRRELDLSELEVITALELVTEEYGADRSR
                     TFLAGHSMGSGGVWHLAARYPERWRAVAPMSGPFIDKESYPFDLIRRLPIFVTEGTGA
                     TPSLAGSRALAEFMRAGDFDFEYLEVDGNHGTMVPMVWPAIFEFFERVAARAHR"
     gene            1058..1987
                     /locus_tag="VFL84_00575"
     CDS             1058..1987
                     /locus_tag="VFL84_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HET7607142.1"
                     /translation="MRARVSRLVICTGTALWSLAAAHAQTEIRLWPGKAPGSERWSIA
                     EATTRSPSGDRVITNVSDPTLTAFLPDPKLANGAAAVIAPGGALRALAFDNEGVKLAA
                     WLNERGIAGFVLKYRTLQQDPSAPRGPAAGIPGPAGPRQELAIVHGNANPAPNDAALA
                     TVLELAVADAQAALKLVRARADEWHVDPRRVGIIGFSAGGGVAVGTALAPQSDASPAF
                     LVSVYGPSLQDVDVPAHAPPLFVAVGAAHFNVTNGCLALFAAWKAAGKPAEIHIYDGI
                     SAGFGMTKRGSPVDGWTDRLHEWLVARELTRQN"
     gene            2015..3031
                     /locus_tag="VFL84_00580"
     CDS             2015..3031
                     /locus_tag="VFL84_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7607143.1"
                     /translation="MKSSTRLARSFGFALVIGHLGGVTTAALAQTPAAAAITKLTAVE
                     YQQTGQPPTGPFAIVIEHDPGLATHTIYRPRELTLDKHPVLVWGEGGCAKNGLFFPEY
                     LSEIASHGFVVIADGPPIPRPAGARPPGGGGPPPGAGGPPPGGGPPPGGAGGPPADRF
                     QMVNGAALVAAMDWLEAQSKDRGSRFYKKVDVERVAAMGMSCGGLMSYGASNDPRVAT
                     VGIWNSGLFEDERNAKIYAGIHSSVIIVTGGESDIAYENGKRDFEVMPPRVPVFYGVH
                     PAVGHGGTYNQDNGGPFGVVAVAWLKWQLQGDKSAAGRGYFVGDNCGICKDASWKVAS
                     RALP"
     gene            complement(3044..>3516)
                     /locus_tag="VFL84_00585"
     CDS             complement(3044..>3516)
                     /locus_tag="VFL84_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7607144.1"
                     /translation="VPAMLRGAEEVFEYPMIDRDPVSTWVDGRVALLGDAAHVMYPTG
                     SNGASQAIVDSRVLGAAMLEHGVTEAALQAYNRKLCAEISAVVLRNRGAGPFGLLNLL
                     DERCGGVFENIDAVIPPQERDEFMSRYRSAAGFAKDRLNAAPPTIAPGAKVAAT"
BASE COUNT          544 a         1205 c         1203 g          564 t
ORIGIN      
        1 cgggctggcg ggcagcttga tgccgcgcgg cgaaacgggc aatgcgatgg gcatcgtgag
       61 cctcggcgcg ggcctgttcg gctatttcgg gccgcagatg ctaggtatcc tgcgcgacgc
      121 gacgggctcg ttcgcggcgg gcttctacat gatcgcggcg tgcgacctga tgacactcgc
      181 cttgatcgtt gtgctgtatc gactgacgcg aagcgcgaga tagaaggaga cgacaatgaa
      241 ccgatccgtg ttcgtcttca tcgtcgcctg gctgggcgtc accgctactg ctgcgcagtc
      301 tgcgcccgcg accggcgatc agcaccgcac ctacgtgttc gcgccgacgg gcgcggagat
      361 gccctatcgc gtctacgtcc cgaccagctg ggacggcaag cgctcgctgc cgattctctt
      421 gttcctgcac ggcgccggcg caaacgagag cacgtacctc gacatcgcgg acggcctgct
      481 gcgcaagctc gccgagcaac acggctacat cgtcgtctcc ccgctcggct tcacgccgct
      541 cggcgcctac ggcaacccgt tgcgcctgcc cgccgtgttt ggcgagtcgg aagctgcggc
      601 cgcgcagcgc gcggccgtca cgccgcagcg ccgccgcgag ctcgacctga gcgagctcga
      661 ggtcatcacg gcactcgagc tcgtgaccga ggaatacggc gcggaccgat cgcgaacgtt
      721 cctcgcggga cactcgatgg gcagcggcgg cgtctggcac ctcgccgcgc gctatccgga
      781 acgctggcga gccgtcgcgc cgatgtcggg tccgttcata gataaggagt cgtatccgtt
      841 cgacctgata cggcgcctac cgattttcgt gaccgagggc actggcgcca cgccgtcgct
      901 cgccggtagc cgcgcgctcg ccgagttcat gcgcgccggc gacttcgact tcgaatatct
      961 cgaggtcgac ggcaatcacg gcacgatggt cccgatggtt tggcccgcga tcttcgagtt
     1021 cttcgagcgc gttgccgcgc gcgcacacag atagacaatg agagcgcgcg tcagccgact
     1081 cgtgatctgc accggcaccg cgctgtggtc gctggcggcc gcgcacgccc aaaccgagat
     1141 ccgtctctgg cccggcaaag cgcccggctc cgagcggtgg tcgatcgccg aagcgacgac
     1201 gcggtcaccg tccggcgacc gcgtcatcac gaacgtcagc gatccgaccc tgaccgcctt
     1261 cttaccggac ccgaagctcg cgaacggtgc agcagccgtc atcgcgccgg gtggcgcgct
     1321 gcgcgcgctc gcgttcgata acgaaggcgt gaagctggcc gcgtggctca acgagcgcgg
     1381 catcgccggc ttcgtgctga agtaccgcac gctgcagcag gatccgtcgg cgccgcgcgg
     1441 ccccgcggcg gggatcccgg gaccggcggg cccgcggcag gagctcgcca tcgtccacgg
     1501 caacgcgaac cctgcgccga acgacgcggc gctcgccacg gttctcgagc tcgccgtcgc
     1561 cgatgcgcaa gcggcgctga agctcgtgcg tgcgcgcgcc gatgaatggc acgtcgatcc
     1621 gcggcgcgtc ggcatcatcg ggttttcggc cggcggcggc gtcgccgtcg gcacggcgct
     1681 ggcgccgcaa tcggacgcgt cgcccgcttt cctcgtctcc gtctatggcc cgtcgctgca
     1741 agacgtcgac gtgcccgcgc acgcgccgcc gctgttcgtc gcagtcggcg ccgcgcactt
     1801 caacgtcacg aacggctgcc tcgcgttgtt cgcggcatgg aaagccgcgg gcaagcccgc
     1861 ggagatccac atctacgacg gcatcagcgc ggggttcgga atgacgaagc gcggcagccc
     1921 cgtggacggc tggaccgatc gtctacacga atggctggtg gcgcgcgagc tgacgcgcca
     1981 gaattgatcc tgatcgaacc actggggggt acaaatgaaa tcgtcgactc gcttggcacg
     2041 atcgttcgga ttcgcgctcg tgattggtca tctcggcgga gttacgaccg cggcgctggc
     2101 gcagacgccg gcagccgccg cgatcacgaa gctcacggct gtcgagtacc agcagaccgg
     2161 ccaaccgccg accggcccgt tcgcgatcgt catcgagcat gaccccggcc tcgcgacgca
     2221 cacgatctac cggccgcgag agctgacgct cgacaagcac cccgtgctcg tctggggcga
     2281 aggcggttgc gcgaagaacg ggctgttttt ccccgaatac ttgagcgaga tcgcgtccca
     2341 cggcttcgtc gtcatcgcgg acggcccgcc gattccacgc ccggccggtg cgcggccccc
     2401 gggtggcggt ggtcctcctc ccggtgccgg cgggcctccc cccggtggcg gacctcctcc
     2461 gggcggcgcc ggcggtccgc cggcggaccg cttccagatg gtgaacggcg ctgccctcgt
     2521 cgccgcgatg gactggctcg aggcgcagag caaggaccgc ggcagccgct tctacaaaaa
     2581 ggtcgatgtc gagcgcgtcg cggcgatggg catgtcttgc ggcggcttga tgtcgtacgg
     2641 agcgtcgaac gatccgcgcg tcgcgacggt cgggatctgg aacagcggtc tgttcgagga
     2701 cgagcgcaac gcgaagatct acgcgggcat tcacagctcc gtgatcatcg tgacgggcgg
     2761 cgaatcggac atcgcgtacg aaaacggcaa gcgcgacttc gaggtcatgc cgccgcgcgt
     2821 tccggtcttc tacggcgtgc atccggcggt cggccatggc ggcacttaca accaggacaa
     2881 cggcgggccg ttcggcgtcg ttgccgtcgc gtggctgaag tggcagctcc agggggacaa
     2941 gagcgcggca ggccgcggct acttcgtcgg cgacaattgc gggatctgca aggatgcgag
     3001 ctggaaggtc gcttcgcgag cgctgccctg aagcgcgagc cgatcaagta gccgcgactt
     3061 tcgcgccggg cgcgatcgtc ggcggcgccg cgttcagccg gtccttggca aaaccggctg
     3121 cagagcggta gcggctcatg aactcgtcgc gctcttgcgg cgggatgacg gcgtcgatgt
     3181 tttcgaagac gccgccgcaa cgctcgtcga gcaaattcaa gaggccgaac gggccggctc
     3241 cgcggttgcg cagcacgacc gctgagatct ccgcgcatag ttttcggttg taggcttgca
     3301 gcgccgcttc cgtgacgccg tgctcgagca tcgccgcccc gagcacgcgc gagtcgacga
     3361 tcgcttggct ggcgccattc gagccggtcg gatacatgac gtgggccgcg tcgccgagca
     3421 gcgctacgcg gccgtcgacc cacgtcgaga ccgggtcacg gtcgatcatc gggtattcga
     3481 acacttcctc cgcgccgcgc agcatcgcgg gcacgt
//
LOCUS       DASSNN010000031         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_134104, whole genome shotgun sequence.
ACCESSION   DASSNN010000031 DASSNN010000000
VERSION     DASSNN010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134104"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1820
                     /locus_tag="VFL84_00590"
     CDS             <1..1820
                     /locus_tag="VFL84_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7607145.1"
                     /translation="ELVGGKRFDVALTGRNVDATTGTASVKPVGDLRVVGTSVPRYDI
                     PGKVDASLTWAVDMKVPGMLHARNVRPPVAGATLKGIDEASVKQMPGFVRVVRGGNYV
                     AVVCEREEQAIAASRAVKCEWTPPATAPFPASTELFNYIRGATPTSEETDVDGDPDAA
                     FASAARVLEAEYEVPFQGHTAIGPAHAMADPTNDQLTIYSNDMKSYGLRNGVAQFLGV
                     PRERVRVVYMDGPQVYGRTAADDVGFEAAFLAKELGRPVRVQWMRHEETAWDTKGPPY
                     VFKLKGALSAQGKLTALDYDARAVDYAHLGYNEPDTVLIAQLMGKRPERIAPGDAARP
                     SVKYAIPNQRMASHVVALPLVWETPLRTGNLRDPNGPQVTFAFEGFIDELAAAAKADP
                     VQFRLEMLEASAEDNVFRKARSLAVVKAAAEKYGWDARPSPKPQAAGNVATGRGIAYT
                     YRSNTVVAVIAEVEVDRETGRVWVKRLVCAHDCGLAINPRGLRNTVECGMLHGLSRAL
                     HEEVQFDAEKVTSVDWATHPSLRHSDTPEKIDIVLVNGDPNPNRPDLPHYGAGETSHK
                     PIIAAVANAVYDATGVRLRRAPFRKERVLAALRAAGANT"
     gene            1955..2413
                     /locus_tag="VFL84_00595"
     CDS             1955..2413
                     /locus_tag="VFL84_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7607146.1"
                     /translation="MSIEARSLLVVVAGCLAASAALAFEAVTLEDYSGEELFDRFCAS
                     CHGAQARGDGPVSRSLNVAVPDLTRIAARYGEFPTAVIRDVIDGRGIDMRAHGTREMP
                     VWGYEFWVEEGADVAAQRTVRDAINKLVEYLRSIQRGEDDARTPESVARR"
BASE COUNT          418 a          846 c          894 g          358 t
ORIGIN      
        1 ccgagctcgt cggcggcaag cggttcgacg tcgcgcttac cgggcgcaac gtcgatgcga
       61 cgacgggcac cgccagcgtc aagcccgtgg gcgacttgcg cgtggtcggc acgtcggtgc
      121 cgcgctacga cattcccggc aaggtcgacg cgtcgctgac gtgggccgtc gacatgaaag
      181 tgcccggcat gctgcacgcg cgcaacgtgc gcccgccggt cgccggcgcg acgttgaagg
      241 gcatcgacga ggcgtcggtc aagcaaatgc ccggtttcgt gcgcgtcgtg cgcggcggca
      301 actatgtcgc cgtcgtctgc gagcgcgagg agcaggcgat cgcggcctcg cgcgcggtca
      361 agtgcgagtg gacgccgccg gcgacggcgc cgttcccggc gtcgacggag ctattcaatt
      421 acatccgcgg cgcaacgccg acgagcgagg agacggacgt cgatggcgac ccggacgccg
      481 cgttcgcgag cgccgcgcgc gtgctcgagg ccgagtacga ggtgccgttc caaggccaca
      541 cggcgatcgg ccccgcgcat gcgatggcgg atccgacgaa cgatcagctc acgatctatt
      601 cgaacgacat gaaatcgtac ggcttgcgca acggcgtcgc acagttcctc ggcgtgccgc
      661 gcgagcgcgt gcgcgtcgtc tacatggacg ggccccaggt ttacggccgc acggccgcgg
      721 acgacgtcgg tttcgaggcc gcgttcctcg cgaaagagct gggcaggccg gtccgtgtgc
      781 agtggatgcg ccacgaggag acggcctggg atacgaaagg cccgccctac gtgttcaagc
      841 tcaaaggcgc gttgagcgcg caaggcaaac tcacggcgct cgactacgac gcgcgcgccg
      901 tcgactacgc gcacttgggc tacaacgagc ccgacacggt gctgatcgcg cagctcatgg
      961 gcaagcgccc cgagcgcatc gcgccgggcg acgctgcgag gccgtcggtc aagtacgcga
     1021 ttccgaacca gcgcatggca tcgcacgtcg tcgcgctgcc gctggtgtgg gagacgccgc
     1081 tgcgcacggg caacctgcgc gacccgaacg gcccgcaagt gacgttcgcg ttcgaaggct
     1141 tcatcgacga gctcgcggcc gcggcgaaag ccgaccccgt gcagttccgc ctcgagatgc
     1201 tcgaggcgag cgcggaggac aacgtgttcc gcaaagcgcg gtcgctcgcc gtcgtgaagg
     1261 ccgccgccga gaagtacggc tgggacgcgc ggccctcgcc gaagccacag gccgcgggca
     1321 acgtcgcgac gggccgcggc atcgcgtaca cgtaccgcag caacacggtc gtcgccgtga
     1381 tcgccgaggt cgaagtcgat cgcgagacgg gccgcgtctg ggtgaagcgc ctcgtctgcg
     1441 cgcacgactg cggtctcgcg atcaatccgc gcggcctccg caacacggtc gagtgcggca
     1501 tgttgcacgg gttgagccgc gcgctgcacg aagaggtgca gttcgacgcc gagaaggtca
     1561 cgagcgtcga ctgggccacg cacccgagcc tgcgccactc cgatacgccg gagaagatcg
     1621 acatcgtgct cgtcaacggc gacccgaacc cgaatcgccc ggacttgccg cactacggcg
     1681 ccggcgagac gagccacaag ccgatcattg cggcggtcgc gaacgcggtg tacgacgcga
     1741 cgggcgtgcg cttgcggcgc gcgccgttcc gcaaggagcg cgtgctcgcg gcgctgcgcg
     1801 cggcgggcgc gaacacgtag ccgtcacaag gcgacgctcg cgcgggtaag tacaacccgc
     1861 agcgctcggc tgcggtcctc ggtgtaactt gatcgcgcgg ttgctgctcc ggtccgcggt
     1921 aagctcgagg aactcgcgtt cgaggtgcca gaacatgtcg attgaagcgc gctcgttgct
     1981 agtcgtggtt gcgggttgct tggccgccag cgccgcgctc gcgttcgagg ccgtcacgct
     2041 cgaggactac agcggtgaag agcttttcga ccgcttctgc gcgagctgtc atggcgcgca
     2101 agcgcgcggc gacggcccgg tgagccgctc gctgaacgtc gccgtgccgg atctcacgag
     2161 aatcgcggcg cgttacggcg agtttccgac ggccgtgatc cgcgacgtca tcgacggccg
     2221 cggcatcgac atgcgtgcac acggcacgcg agaaatgccg gtctggggtt acgagttctg
     2281 ggtcgaagag ggcgccgacg tggctgcgca gcgcaccgtc cgcgacgcaa tcaacaagct
     2341 cgtcgagtat ctgcgctcca ttcagcgcgg cgaggacgac gcacgcacgc cggaatcggt
     2401 cgcgcgccgg taatcgctcg tcgagaacac cgtgcccgaa gcttacgtcg ccgcgctcga
     2461 cagcgtctcg atgcgcgacg tcgcgcgcgt cggcggcaag aatcgcctcg cggcga
//
LOCUS       DASSNN010000032         3841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1142449, whole genome shotgun sequence.
ACCESSION   DASSNN010000032 DASSNN010000000
VERSION     DASSNN010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142449"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFL84_00600"
     CDS             complement(<1..443)
                     /locus_tag="VFL84_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006483697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607147.1"
                     /translation="MTTRRSVLAYGATLTLLAPFTGLQAAANPDSRFVLVILRGGLDG
                     LAAVPPYAEPAYAQLRGPLALPKPGTQDGALDLDGTFGLHPALANLHAMYRAKEALVL
                     HAAATPYRERSHFDAQNVLEDGGTAPNASGGGWLNRALAALKSAG"
     gene            complement(446..1909)
                     /locus_tag="VFL84_00605"
     CDS             complement(446..1909)
                     /locus_tag="VFL84_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HET7607148.1"
                     /translation="MLTKDAAIAANRFGLGARPGDARAIGSDAKGWLLEQLAHAPSKA
                     PAGAPESARVLAEVRDLRVARQIAQRARANLVRAPNSDAPPPAPGIDQQAIREFGAFV
                     RERYVAQTAERHKLAIETERPFVERLVHFWSNHFAVSADKQPLAALAGLYEQEAIRPH
                     VTGNFYDLLLAVERHPAMILYLDNEASMGPSSQAARAARVVRRNGGPELGLNENLGRE
                     ILELHTLGVDGGYTQADVTEFAKVLTGWSIGGGLGEGRPLLARLGGDAGAPGEFHFRA
                     PMHEPGDKMILGKRYRERGVDEGEDVLRTLALHPKTARHLATKLARHFVADDPPATLV
                     GRMADAYSKSEGELTSVYRVLIEADESWREPLAKLKTPHDFVISAYRLLGSRPDNLQP
                     ITAFLTEAGQRPYAPGSPAGWPDTAASWNGGDALLKRIELATAVGRRVGSSIDPSARA
                     GEVLGALGEHTAMSIRGAESGAQAFALLLASPEFQRR"
     gene            complement(1987..3771)
                     /locus_tag="VFL84_00610"
     CDS             complement(1987..3771)
                     /locus_tag="VFL84_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7607149.1"
                     /translation="MKRASIAVGLLAMAVATAPPAQPSDASPRLGAVAAAPVTDAMLR
                     NPDPADWLMYSRTYDAQRYSPLTQINRDNVGSLARAWSKPLPEGPLEIIPLVYRGVMY
                     LTTPGSRAGGSRVVALDAATGETLWEYVPPNNASSRIKSLAIYGDSIYYTAPAPSGEP
                     NPVVALDAATGAVRWQTPVTVETHTAGAIVVEGKVISGRACNTARSNCYIAAHDARTG
                     KEAWRFYTSPGAGEPGDASWGGKPVDERRAAAWGLPGTYDPQRRLVYWGIANPMPNTR
                     AERHGGNVDAVARRAPADLYSNSTVALNPDTGELVWHYQHLPGDDWDMDINEERVLIR
                     TPVDPDPRHVKWINPNVRKGVERDVAITVGEGGGVWLNDRATGEFLWAMPFPYDTEHF
                     IIEDIDVETGVAHINSKVLLEEPGQTKLVCYWNTRSFWPTAYSPKLNSLYVPYIDHCL
                     SMTRASLSGDPERRTSGLRPGADQAKLAGIAKIDMRTGEIRRLYESSIAGNGAMLATA
                     GDLLFWGDIGQVLRAFDAETGKVLWQSEPLGATVQTSTITYAVDGKQYVAVVNAESLL
                     GAPRLAAVAGASIPPHRVSSINVFALPR"
BASE COUNT          547 a         1307 c         1364 g          623 t
ORIGIN      
        1 tcgccggcgg acttcagcgc cgcgagcgcg cggttcaacc agccgccgcc gctcgcgttc
       61 ggcgccgtgc cgccgtcctc gagcacgttc tgcgcgtcga agtgcgagcg ctcgcggtac
      121 ggtgtcgccg cggcgtgcag cacgagcgct tccttcgcgc gatacatcgc gtgcagattc
      181 gcgagcgcgg gatgcaggcc aaacgtgccg tcgagatcga gcgcgccgtc ttgcgtgccg
      241 ggcttgggca gcgcgagcgg gccgcgcaat tgcgcgtacg cgggctcggc gtacggcggc
      301 acggcggcaa ggccgtcgag gccgccgcgt agaatgacga gcacgaagcg gctgtcgggg
      361 ttcgccgccg cttgtaggcc cgtgaacggc gcgagcagcg tcagcgtcgc gccgtaggca
      421 agcaccgagc gtcgagtcgt catcgtcatc tcctttgaaa ctcgggcgac gcgagcagaa
      481 gcgcgaacgc ttgggcgccg ctttcggcgc cgcgaatgct catcgccgtg tgctcgccga
      541 gcgcgccgag cacttctccg gcgcgcgcgc tggggtcgat gctgctgccg acgcgccggc
      601 ctaccgcggt cgcgagctcg atgcgcttca gcagcgcgtc gccgccgttc cagctcgcgg
      661 ccgtgtcggg ccagcctgcc ggcgagcctg gcgcgtacgg gcgctgcccg gcttccgtca
      721 ggaatgcggt gatcggctgc aagttgtcgg ggcgcgagcc gagcaggcgg tacgccgaga
      781 taacgaagtc gtgcggtgtc ttgagcttcg cgagcggctc gcgccagctc tcgtcggcct
      841 cgatcaaaac gcgatagacg ctcgtgagct cgccttcgct cttcgagtac gcgtccgcca
      901 ttcggccgac gagcgtcgcg ggcggatcgt cggcgacgaa atgccgcgcg agcttcgtcg
      961 cgagatggcg cgccgtcttc ggatgcagcg cgagcgtgcg cagcacgtcc tcgccttcgt
     1021 cgacgccgcg ctcgcggtag cgtttgccga gaatcatttt gtcgcccggc tcgtgcatcg
     1081 gcgcgcggaa atggaattcg cccggagcgc cggcgtcacc gcccaagcgc gcgagcagcg
     1141 ggcggccttc gccgagcccg ccgccgatcg accagcccgt cagcaccttc gcgaattccg
     1201 tgacgtcggc ttgcgtgtag ccgccgtcga cgccgagcgt gtgcagctcg aggatctcgc
     1261 ggccgagatt ctcgttgagc ccgagctcag gcccgccatt acggcgcacg acgcgcgcgg
     1321 cgcgcgcggc ttgcgagctc ggccccatcg aggcttcgtt gtcgagatac aagatcatcg
     1381 cgggatgccg ctcgacggcg agtagcagat cgtagaagtt gcccgtgacg tgcggccgga
     1441 tcgcttcctg ctcgtagagg cccgcgagcg ccgcgagcgg ctgcttgtcg gccgagacgg
     1501 cgaagtggtt gctccagaaa tgcacgagcc gctcgacgaa cggccgctcc gtctcgatcg
     1561 cgagcttgtg ccgctcggcc gtctgcgcga cgtagcgctc gcgcacgaac gcgccgaatt
     1621 cccgaatcgc ctgctggtcg atgccgggcg caggcggcgg tgcgtcggaa ttcggagcgc
     1681 gcacgaggtt cgcgcgcgct cgctgcgcga tctgccgggc gacgcgcagg tcgcgcacct
     1741 cggcgagcac gcgcgccgac tccggagcac cggcgggcgc cttggacggc gcatgcgcga
     1801 gctgctcgag cagccagcct ttcgcgtcgg agccgatcgc acgcgcgtcg ccgggcctcg
     1861 caccgagccc gaaccggttc gcggcgatcg cggcatcttt tgtcaacatc ggcggtcctc
     1921 gatcgaagga gcgtactcgc tgcaacggcc gaagccgggc gccggtttac aagggctacg
     1981 gggttttcac cgcggcagcg caaagacgtt gatcgagctc acgcggtgcg gcgggatcga
     2041 agccccggcg accgcggcca gccgcggcgc gccgagcagc gactcggcgt tgacgacggc
     2101 gacgtactgc ttgccgtcga ccgcatacgt gatcgtgctc gtctgaaccg tggcgccgag
     2161 cggctcggac tgccaaagga ccttgcccgt ctcggcgtcg aaggcgcgca ggacctggcc
     2221 gatgtcgccc cagaacaaaa ggtcgccggc cgtcgcgagc atcgcgccgt tgcccgcaat
     2281 gctggattcg tagaggcgcc ggatctcgcc cgtccgcatg tcgatcttcg cgatgccggc
     2341 gagcttcgcc tggtcggcac cggggcggag gccgctcgtg cgccgctcgg ggtctccgct
     2401 caagctcgcg cgcgtcatgc tcaagcagtg atcgatgtac ggcacgtaca gcgaattcaa
     2461 tttcggcgaa tacgccgtcg gccagaagct gcgcgtgttc cagtagcaga cgagcttcgt
     2521 ctgcccgggc tcctcgagca gcaccttcga gttgatgtgc gcgacgccgg tctcgacgtc
     2581 gatatcctcg atgatgaagt gctcggtgtc gtacggaaac ggcatcgccc agaggaactc
     2641 gcccgtcgcg cgatcgttga gccagacgcc tccgccttcg ccgacggtga tcgcgacgtc
     2701 gcgctcgacg cccttgcgca cgttcgggtt gatccatttg acgtggcgcg gatcgggatc
     2761 gacgggcgtg cgaatcagca cgcgctcctc gttgatgtcc atgtcccagt cgtcgccggg
     2821 caaatgctgg tagtgccaga cgagctcgcc cgtgtccgga ttcagcgcga cggtcgagtt
     2881 gctgtaaagg tcggccggcg cgcggcgcgc aacggcatcg acgttgccgc cgtgccgctc
     2941 cgcgcgcgtg ttcggcatcg gattcgcgat gccccagtag acgagccgac gctgagggtc
     3001 gtacgtgccg ggcaagcccc acgcggccgc tcggcgctcg tcgacgggct tgccgcccca
     3061 cgaagcgtcg ccgggctcgc cggcgcccgg gctcgtgtag aagcgccacg cttccttgcc
     3121 ggtgcgcgcg tcgtgcgccg cgatgtagca attcgagcgc gccgtattgc aggcgcggcc
     3181 cgagatgacc ttgccttcga cgacgatcgc gccggcggtg tgcgtctcga cggtcacggg
     3241 cgtttgccag cgcacggctc cggttgcggc gtcgagcgcg acgacggggt tcggctcgcc
     3301 gctcggcgcc ggtgccgtgt agtaaatcga gtcgccgtaa atcgcgagcg acttgatgcg
     3361 cgacgacgcg ttgttcggcg gcacgtattc ccacaacgtc tcgccggtcg cggcgtcgag
     3421 cgcgacgacg cggctgccgc cggcgcggct gcccggcgtc gtcaaataca tgacgccgcg
     3481 atagacgagc ggaatgatct cgagcggacc ctcgggcagc ggcttcgacc acgcacgcgc
     3541 gagcgacccg acgttgtctc ggttgatttg cgtgagcggg ctgtagcgct gcgcgtcgta
     3601 ggttcggctg tacatgagcc aatcggcggg atcgggattg cgcagcatcg cgtcggtgac
     3661 gggagcggcc gcgactgcgc cgagcctcgg gcttgcgtcc gacggctgcg ccggcggcgc
     3721 ggttgccacc gccatggcga gcagaccgac cgcaatcgaa gcgcgtttca tgaacgtccc
     3781 cccaacctga gctcacgacg atggtaacgg gttcgccgag cggcgcccat tgcgcttgaa
     3841 g
//
LOCUS       DASSNN010000033         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_134777, whole genome shotgun sequence.
ACCESSION   DASSNN010000033 DASSNN010000000
VERSION     DASSNN010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134777"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFL84_00615"
     CDS             <1..339
                     /locus_tag="VFL84_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014872183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HET7607150.1"
                     /translation="WQGRSFVRKALEAYFAVLLELCLTKERILELYLNVAELGPQVFG
                     VEAAAREYFGTTASALDLEQAALLAAVLPNPHRLLAAQPSEYVRGRQAEIVAEVELLE
                     SRGHYRGIHW"
     gene            336..872
                     /locus_tag="VFL84_00620"
     CDS             336..872
                     /locus_tag="VFL84_00620"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HET7607151.1"
                     /translation="MSRARVELSVGFPPIEDASARVLVLGSLPGRKSLEMREYYAQPY
                     NAFWRIMGELFGAGPALEYPARMVRLRAHGVAVWDVLAAGEREGSLDSSIVRASIVVN
                     DFHSFFAHHRELELICFNGNTAARLFERKVLPGLPPEWAALERRALPSTSPAYASLRF
                     EQKLARWRAALSGPSAAL"
     gene            888..1781
                     /locus_tag="VFL84_00625"
     CDS             888..1781
                     /locus_tag="VFL84_00625"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7607152.1"
                     /translation="MGTTNSGWIYRARVSSDAAGASVLAFHVARYRHSGEVEWRAAIE
                     LGRVLVNGRRVRPEQPLEAGDELEFHRAPWREPDAPESFTVAFEDAHVLVVVKPANLQ
                     VLPAGPFSARTLLALVRSSDASRAEAAPAHRLGRGTSGLIAFGKTAAARSSLSRQLRE
                     LTLGKTYLAWTCGERLPSSFAARQPIARVPHGPLTIHAAAAHGRPSLTRVRVLRRAGA
                     RTLVAAQPITGRPDQIRIHLAAAGAPIVGDPLFGPGGVPKSDAPPGAGGYLLHSAGLK
                     FAHPESGARVTLRSLPDWLDG"
     gene            1784..>2433
                     /locus_tag="VFL84_00630"
     CDS             1784..>2433
                     /locus_tag="VFL84_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607153.1"
                     /translation="MVSEKSSTLRLAAVLMAAALSACRAEPPPTAPAFRARAVAFPGA
                     AGSAQPRLNVGADGLAILSWLEPAGDGSVLRYAVFDGAAFSMPREVVRGDDLMVNWAD
                     MPSVQPITADVWAAHWLRLDPEQAGAYHIATAVSNDGGVTWSGQVQLNDDSAAAEHGF
                     VDLFAWNGAIGAFWLDGRQLAEWSFDEPDKLLGTSLRVAELDYSGNVVAREIVDELV"
BASE COUNT          311 a          860 c          881 g          381 t
ORIGIN      
        1 tggcaaggcc gcagcttcgt gcgcaaagcg ctcgaggctt acttcgccgt gctgctcgag
       61 ctgtgcttga cgaaggagcg catcctcgag ctctacctca acgtcgccga gctcgggccg
      121 caagtattcg gcgtcgaagc ggccgcgcgc gagtacttcg gcacgaccgc gagcgctctc
      181 gacctcgagc aggccgcgct gctagcggcc gtgctgccga acccgcatcg attgctcgcc
      241 gcgcaaccga gcgagtacgt gcgcgggcgg caagccgaga tcgtcgccga ggtcgagctg
      301 ctcgagtcgc gcggccacta tcgcggaatc cattggtgag ccgcgcgcgc gtcgagctgt
      361 ccgtcggttt cccgccgatc gaagacgctt cggcgcgagt gctcgtgctc ggcagcctgc
      421 ccggccgcaa gtcgctcgag atgcgcgagt actacgcgca gccgtacaac gcattctggc
      481 gcatcatggg cgagctcttc ggcgccggcc ccgcgctcga gtacccggcg cgaatggtgc
      541 ggctgcgcgc gcacggcgtc gcggtctggg acgtgctcgc ggccggcgag cgcgagggca
      601 gcctcgattc ctcgatcgtg cgcgcgagca tcgtggtcaa cgactttcac tcgttcttcg
      661 cgcaccaccg cgagctcgag ctgatctgct tcaacggcaa cacggcggcg cgcctgttcg
      721 agcgcaaggt gttgcccggg cttccgcccg agtgggcggc gctcgagcgg cgggcgctgc
      781 cgtcgacgag ccccgcctac gcgagcttgc ggttcgagca gaagcttgcg cgctggcgcg
      841 cggccctgag cggtccctcg gctgctcttt gatctatgct gagcggcatg ggcacgacga
      901 attcgggctg gatctatcgc gcgcgcgtgt cgagcgatgc ggcgggcgcg agcgtgctcg
      961 cgttccacgt cgcgcggtat cgccactccg gcgaggtcga atggcgtgcc gcgatcgagc
     1021 tcggccgagt gctcgtcaac ggccgtcgcg tgcggcccga gcagccgctc gaggccggcg
     1081 acgagctcga gttccaccgc gcgccgtggc gcgagcccga cgcgcccgag tcgttcacgg
     1141 tcgcgttcga ggatgcgcac gtgctcgtcg tcgtcaagcc cgccaatctg caagtgctgc
     1201 cggcgggccc gttctcggca cgcacgcttc ttgcgctcgt gcgctcgagc gatgcgtcgc
     1261 gcgcggaggc cgcgccggcg cataggctcg gccgcggcac gtcggggctc atcgcgttcg
     1321 gcaagacggc ggccgcgcgc tcgtcgctga gccgccagct gcgcgagctg acgctcggca
     1381 agacctatct cgcgtggacc tgcggcgagc gcctgccgag ctcgtttgcg gcgcggcagc
     1441 cgatcgcgcg cgtgccgcac ggcccgctga cgattcacgc cgccgcggcg cacgggcggc
     1501 cgtcgctgac acgcgtgcgc gtgctgcggc gcgccggcgc gcgcacgctc gtcgcggcgc
     1561 agccgatcac gggccgcccc gatcagatcc gcattcacct cgccgcggcc ggcgcgccga
     1621 tcgtcggcga tccgctgttc gggccgggcg gagtcccgaa gagcgacgcg ccgcccggag
     1681 ccggcggcta cttgctgcac tcggccgggc taaaattcgc tcatcccgag agcggcgcgc
     1741 gcgtgacgct gcgctcgctg cccgactggt tggatggatg agcatggttt ccgaaaaatc
     1801 gtcgacgctg cgcctcgcgg ccgtcctcat ggccgccgcg ctcagcgcgt gtcgcgcgga
     1861 gccgccgccc acggcgcccg cgtttcgcgc gcgcgcggtt gcgttcccgg gcgcggcagg
     1921 cagcgcgcag ccgcggctca acgtcggcgc cgacggcttg gccatcttga gctggctcga
     1981 gccggccggc gacggcagcg tgttgcgtta tgccgtgttc gacggcgccg cgttctcgat
     2041 gccgcgcgag gtcgtgcgcg gcgatgacct catggtgaac tgggccgaca tgccgtccgt
     2101 gcagccgatt acggccgacg tctgggccgc gcactggctg cggttggacc cagagcaggc
     2161 aggcgcgtat cacatcgcga cggccgtctc gaacgacggc ggcgtcacgt ggtccggcca
     2221 ggtgcagctc aacgacgaca gcgcggccgc cgagcacggc ttcgtcgatt tgttcgcgtg
     2281 gaacggcgcg ataggggcgt tctggctcga cgggcggcag ctcgccgaat ggtcgttcga
     2341 cgagcccgac aagctgctcg gcacgagctt gcgagtcgcc gagctcgact actcgggcaa
     2401 cgtcgtcgcg cgcgagatcg tcgacgagct cgt
//
LOCUS       DASSNN010000034         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_511962, whole genome shotgun sequence.
ACCESSION   DASSNN010000034 DASSNN010000000
VERSION     DASSNN010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511962"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2454
                     /locus_tag="VFL84_00635"
     CDS             <1..>2454
                     /locus_tag="VFL84_00635"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7607154.1"
                     /translation="HGRAHQAEGRAIDALLCYRLALREAPDGIDARFHLGEIAWHLGN
                     AGDAIAAWQGVAARSTGHLPSLHALADAYAATGQFDAATQAASRVLALAPKEPRASAL
                     AVLLRVARGESVDDETLVPAVQSNPRWPLALLVAACVRALESAQHYAKALPLLLDASE
                     RAPTTHATEDALRVLALALHRAGDMTRASAFAERYAQSCRALHAPAMPMSWPLRATGP
                     SLRVGALVAGNSAAGSIGRLETIGRTLDSRWTCAVFVAAAAEAGMPSSAALDVRALPA
                     DPDVAARGIAALDLDVLLDCTRLQAPIGPLLARHPARAIWIVDDARAPVGKSLADRVV
                     HAPTDEPVAAALAALHADIARQPAASMTVGDLAALWDQSVNAHRGGDAQAAAAGYAKV
                     LAVQPDFAPALYLRSEIARARGDIARAREDLRAAVRVAPGFVDAVAALANLETAAGNA
                     HEAMAVARRGLEHAQSAAALWRALGQAALAAADGHTAAEALSEAAVRDTTHAETHYNH
                     GVALQMAGRPNEAAHAYQRALAFRPDLHDADFNLGVIFDQQGNAGAAIAAFSNVLKRA
                     PSHARAYKALAETLLSSGRIDAWFANFERFERHCPNHLALAAHALEACAYRADFARLE
                     RYLDGLREGRFTEGAPDEIQDALEQLLYLLHFFDVEPALIGRYARMHDTLARKAHGEP
                     RPPHAPRRPGKPRIGYLSGDFRNHVMGKMMWQALRHHDRDAFEVFGYTTTDVRDEWTK
                     RYESIFDSFTSIAARSDASAADRIAADALDVLVDLSTHTKGARPGILARKPARVQITH
                     VASAGTLAMSAIDFKLTDR"
BASE COUNT          364 a          867 c          850 g          373 t
ORIGIN      
        1 gcacggtcgc gcgcatcagg cggagggcag ggcgatcgat gcgctcctgt gctatcggct
       61 cgcgctgcgc gaggcgccgg acggcatcga cgcgcgcttt cacttgggcg agatcgcatg
      121 gcatctcggc aacgccggcg atgcgatcgc ggcatggcaa ggcgtggccg cgcgatcgac
      181 gggccacctg ccgagcttgc atgcgctggc cgatgcctat gccgcgaccg gtcagttcga
      241 cgcggcgaca caagccgcga gccgcgtgct ggcgcttgcc ccgaaggaac cgcgcgccag
      301 cgcgcttgcg gtgctgcttc gcgtcgcgcg aggggaatcc gtcgacgacg agacgctcgt
      361 gcctgccgtc cagtcgaatc cacgctggcc gctggcactg ctcgtggcag cctgcgtgcg
      421 cgcactcgag agcgcgcagc actacgcgaa agcgttgccg ttgctgctcg acgcgtccga
      481 gcgcgcaccg accacgcacg cgacggagga tgcgctgcgg gtacttgcgc tggccttgca
      541 tcgcgcaggc gacatgacgc gcgcttcggc gttcgccgag cgctacgcgc aaagctgccg
      601 tgcgctgcac gcgccggcaa tgccgatgtc gtggccgctg cgcgcgaccg gtccctccct
      661 ccgggtcggc gccctcgtcg caggaaattc ggcggcaggc tccattgggc ggctcgaaac
      721 gatcggccgg accctcgatt cgcgatggac gtgcgcggtc ttcgtcgccg cggcggccga
      781 agccgggatg ccctcatccg cagcgctcga cgtgcgcgcg ctcccggcgg atccggatgt
      841 cgcggcgcgt ggcatcgcgg ctctcgacct cgacgtgctg ctcgattgca cccgtctgca
      901 agccccgatc ggtccgctgc tcgcgcggca ccccgcacgt gcgatctgga tcgtcgatga
      961 cgctcgtgcc ccggtcggga aaagccttgc agatcgcgtc gtgcatgcgc cgaccgacga
     1021 gccggtggcg gcagcgttgg cagcgctgca cgccgacatc gcccggcaac ccgcggcgtc
     1081 gatgaccgtc ggcgatctcg cggcgctttg ggaccagtcc gtgaatgcgc accgcggcgg
     1141 ggatgcgcag gcggcggctg caggttacgc gaaggtgctc gccgtgcagc cggattttgc
     1201 gccggcgctt tacttgcggt cggagatcgc ccgcgcccgc ggcgacatcg cgcgcgcgcg
     1261 cgaagatctg cgcgcggcgg tgcgtgtggc gccgggcttc gtcgacgcgg tcgccgcgct
     1321 cgcgaatctc gaaacggcag caggcaacgc gcacgaagcg atggcggtcg cgcggcgcgg
     1381 actcgagcac gcgcagtccg cagccgcgct ctggcgcgcg ctcgggcagg cggcgctcgc
     1441 ggcggcggac gggcacaccg ccgcggaagc gctctccgaa gccgcggtgc gggacacgac
     1501 gcacgcggag acgcattaca accacggcgt cgcgctgcag atggccggca ggccaaacga
     1561 ggctgcgcat gcgtatcagc gcgctctggc gttcaggccc gatcttcacg atgccgattt
     1621 caatctcggc gtcattttcg accagcaggg caatgcgggc gccgcgattg cagcattctc
     1681 gaacgtgctg aagcgcgcgc cgtcgcacgc acgcgcctac aaggctctgg ccgagacgct
     1741 gctttcgtcg ggaaggatcg acgcgtggtt cgccaacttc gagcgcttcg agcgccattg
     1801 tccgaaccat ctcgcgctcg cggcgcacgc gctcgaagcg tgcgcttacc gcgccgattt
     1861 tgcccgcctc gaacgctacc tcgacggcct gcgcgagggc cgcttcaccg aaggcgcacc
     1921 ggacgagata caggatgcgc tcgagcagct cctttacctg ctgcacttct tcgacgtcga
     1981 gcccgcactg atcggccgtt acgcccgcat gcacgacacg ctggcgcgca aggcgcacgg
     2041 cgagccgcgg cccccgcatg cccctcggcg ccccggcaag ccgcggatcg gctacctttc
     2101 cggcgatttt cgcaaccatg tcatgggcaa gatgatgtgg caggcgctgc gtcatcacga
     2161 tcgtgacgcg ttcgaggtgt tcggctacac gacgaccgac gtccgcgacg agtggacgaa
     2221 gcggtacgag tcgatcttcg acagcttcac gtcgatcgcg gcacgcagcg acgcgagcgc
     2281 cgccgaccga atcgcagcag acgctctcga cgtgctcgtc gacctgtcga cgcatacgaa
     2341 gggcgcgcgt cccggcatcc ttgcgcgcaa gcccgcgcgc gtacagatca cgcatgtggc
     2401 gagcgccggc acgctcgcga tgtcggcgat cgatttcaag ctcaccgatc gcta
//
LOCUS       DASSNN010000035         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_886726, whole genome shotgun sequence.
ACCESSION   DASSNN010000035 DASSNN010000000
VERSION     DASSNN010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886726"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VFL84_00640"
     CDS             complement(<1..910)
                     /locus_tag="VFL84_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607155.1"
                     /translation="MTAQSQLEAYLGEFRQRLKALIVARGAALLAVAALVVTAVAVYF
                     GIRRAFDPQIIVSARVVLLLLLAGIAIGLVALPLRALKRNRGIKDIERRAPDFNGRLE
                     TYDGIVHGPPERATPFLGLLAEDALKLARKIPIALKVPAMQVRVPAVLATVAVALLVG
                     TALFGPDNWRYGVRNLWAGWLVKDTLPPQYLAVLPGDGTVRRGGDLRVTATAEGFEPS
                     RMQVFAQFQEGCNWESAQMTRSADGEFDFTFFALREPMRYYVAAAGLRSPEYAVDVVD
                     LPRITSLKLTYNYPNWTKLEPQVVEPG"
     gene            complement(907..>2289)
                     /locus_tag="VFL84_00645"
     CDS             complement(907..>2289)
                     /locus_tag="VFL84_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607156.1"
                     /translation="GALAPRRPAEIVIHDVTDGAAENWTVESFAASAISGELTASVRS
                     FAPDAATRTVTLTQNGRTVGEQTVEVAAHARADVTFPALDLASGSNRVEVALSPGDDL
                     AGDDRRYLAIKRPEPRKVLIVSPDAKGRAPLYVSAALETLTTLSLTADVRASPLGDPP
                     LLDYSFVVVTDVGTLDAAQATAVEDYVENGGRALLAAGPRSSGLTALPVTGQALRANP
                     QMGAQASVAIGEVDRSHPALRGIEELRSANFTRAVNVEPSEADRVLLRLADGTALLLE
                     RSLGSGRVLLFTSSLDKEWNDLPLQPSFVPLMAGIANELLGGAGFTSEADLGSTLAVR
                     ALGFAGGQIFDPSGDAALGLGAGSEDVLLDQVGFYEVVGGGTSQLVAVNFDGRESDLA
                     PVDAATLERWQGLGVRPGESAAPVPVAAESDKVPRSLGPLLVMLLLMLVVVESLVGNW
                     HLRIRRGVAA"
BASE COUNT          371 a          825 c          770 g          323 t
ORIGIN      
        1 cgccgggctc gaccacctgc ggctcgagct tcgtccagtt cggatagttg tacgtgagct
       61 tcaggctcgt gatgcgcggc agatcgacga cgtcgaccgc gtactcgggg ctgcgcaacc
      121 ccgccgccgc gacgtagtaa cgcatcggct cgcgcaaggc gaagaacgtg aaatcgaact
      181 cgccgtcggc cgagcgggtc atctgcgcgc tttcccagtt gcagccctcc tggaactgcg
      241 cgaacacctg catgcgcgac ggctcgaagc cctcggccgt ggccgtgacg cgcagatcgc
      301 cgccgcggcg caccgtgccg tcgccgggca gcaccgcgag gtactgcggc ggcaacgtgt
      361 ctttgacgag ccaaccggcc cacagattgc ggacgccgta gcgccagttg tcgggcccga
      421 acagcgcggt tccgacgagc agcgcgacgg caaccgtggc gagcacggcc gggacgcgca
      481 cctgcatcgc gggcactttc aacgcgatcg gaatcttgcg cgcgagcttc agcgcatcct
      541 cggcgagcag gcccaagaac ggcgtggcgc gctcgggcgg gccgtgcacg atgccgtcgt
      601 aagtctcgag gcggccgttg aagtccggcg cgcgccgctc gatgtccttg atgccgcggt
      661 tgcgcttgag cgcgcgcaac ggcagtgcga cgagaccgat ggcgatgccg gccaagagca
      721 gcagcagcac gacgcgcgcg ctgacgatga tttgcggatc gaatgcgcgg cggatgccga
      781 agtagaccgc gaccgcggtc acgaccagtg ccgcgaccgc gagcagcgct gcgccgcgcg
      841 cgacgatgag cgctttcagg cgctggcgaa actcgccgag gtaggcttct agctgggact
      901 gggcagtcat gcggccactc ctctacgaat cctaagatgc cagtttccta ccagggattc
      961 cacgacaacg agcataagca gcaacattac gagcaatggc ccgagcgatc gcggaacttt
     1021 gtccgactcc gccgccacgg gcaccggcgc cgcgctctcg ccgggccgca cgccgagccc
     1081 ttgccagcgc tcgagcgtcg cggcatcgac gggcgccaaa tccgactcgc gcccgtcgaa
     1141 attcacggcg acgagctggc tcgtcccgcc gccgacgacc tcgtagaacc ctacctggtc
     1201 gagcagcacg tcctcgctgc ccgcgccgag cccgagcgcg gcgtcgccgc tcggatcgaa
     1261 tatctgcccg cccgcgaacc cgagcgcccg caccgccagc gtgctgccga gatcggcctc
     1321 gctcgtgaac ccggcgccgc cgagcagctc gttcgcaatg cccgccatca atggcacgaa
     1381 cgacggctgc aacggcagat cattccactc cttgtcgagc gacgatgtga acagcagcac
     1441 gcgccccgac cccagcgacc gctcgagcaa cagggcggtc ccatcggcca gacgcaatag
     1501 gacacgatcg gcttcactcg gttcgacgtt gacggcgcgc gtaaagttcg cggatcgcag
     1561 ctcctcgata cctcgcagcg ccgggtgact ccggtcgacc tcgccgatcg cgacgctcgc
     1621 ctgtgcgccc atttgcggat tcgcgcgcag cgcctgcccc gtcacgggca gcgccgtgag
     1681 cccgctcgac cgcggaccgg ccgccagcaa cgcgcggccg ccgttctcga cgtagtcctc
     1741 gacggcggtg gcctgggcgg cgtcgagcgt gccgacgtcg gtcactacga cgaagctgta
     1801 gtcgagcagc ggcggatcgc cgagcggcga cgcgcgaacg tccgccgtca acgacagcgt
     1861 cgtcagagtc tcgagcgcgg cgctcacgta caacggcgcg cggcctttcg cgtccggcga
     1921 cacgatcagc actttgcgcg gctcgggacg cttgatcgcg aggtagcggc ggtcgtcgcc
     1981 ggcgagatcg tcgcccgggg aaagcgcgac ctcgacgcgg ttcgacccgc tcgcgagatc
     2041 gagcgcgggg aacgtgacgt ccgcgcgcgc atgagccgca acctcgacgg tctgttcgcc
     2101 gaccgtgcgc ccgttctgcg tcagcgtcac ggttcgcgtc gcggcatcgg gcgcgaagct
     2161 gcgcacgctc gccgtgagct cgcccgagat cgcggacgcc gcgaagctct cgacggtcca
     2221 gttctcggcc gcgccgtccg tgacgtcgtg gatcacgatc tcggcgggcc gccgcggcgc
     2281 gagcgcgcc
//
LOCUS       DASSNN010000036         3767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_8283, whole genome shotgun sequence.
ACCESSION   DASSNN010000036 DASSNN010000000
VERSION     DASSNN010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8283"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFL84_00650"
     CDS             complement(<1..347)
                     /locus_tag="VFL84_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7607157.1"
                     /translation="MPKPPPGIAERDFQAALRELEGVVGREWLFATDEDVLLYRDGYS
                     PLWGEAEERLPSAAVAPNSVEQVQAIVKIANRYALPLYPISTGRNLTYGGAAPAYTGS
                     VVVDLKRMNRILDV"
     gene            complement(340..777)
                     /locus_tag="VFL84_00655"
     CDS             complement(340..777)
                     /locus_tag="VFL84_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607158.1"
                     /translation="MIDRRRFMQSSVALGAAVATWSASGAETSAGGAPRIVVVDRQLA
                     GSAAFLFERRSRGLAAFEFAGDPAALWMRELEPRLRSGALVIEGYTSAATAFCLEYLA
                     RDYGASAVRREEAAGTVTWILSSSPARRAALAPIDPRGSAFHA"
     gene            complement(813..1637)
                     /locus_tag="VFL84_00660"
     CDS             complement(813..1637)
                     /locus_tag="VFL84_00660"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET7607159.1"
                     /translation="MSRLYFAIASVLSLAAATAGAQERWREYPSIERGWYGRDTHPDE
                     APGEFVVGRLMFPQIRRGYMLGAGPGDWRAGGTAWTVDYPEGDRTFARLLARLTTIDV
                     RLVEQPVNLDDDIDAYYWPFLISGLVGAWDLTDGQALKLREYLLRGGFLLCDSFYGTV
                     EWEGFIASLQRVFPDRPIVELPDTHPIFHAVYDLTKRPQIPTWQHLPRGGYRNDGAIP
                     RWRAIVDDDERVMVMITFNNDVADGWQRADEYDYPQEAANLAISLGVNFAVYALSH"
     gene            1675..3201
                     /locus_tag="VFL84_00665"
     CDS             1675..3201
                     /locus_tag="VFL84_00665"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7607160.1"
                     /translation="MTVQRLSIRGLAAVRIVVAAALVARLPAAADEVVVMTSGAFTAP
                     FEVAAPWFERRTGHDVVAVFGASTGGAPDSIPVRLARGERADVVIASAQAVDALIAAG
                     AVERGSRRDLVLSRIGVAVRAGARKPDISSVDALVRAVREARSIAYSASVSGTYLATE
                     LFPRLGLAAEIAAKGRRIESERVGAVVARGDAELGFQQISELVTIDGLEYVGPLPDEV
                     QRVSTFSAGIAAGAKSPPAARELVAFLASIALAPVVERYGLDQVEYTASDWRPLFNGR
                     DLAAWTPKIRRHALGDNFAGTFRVRDGMIEVGYEGYDTFAEQFGHLFYREPFSRYRLR
                     VEYRFVGAQAPDAPAWAARNSGVMVHSQAPETMLRDQDFPISIEVQFLGGLGDGKARP
                     TGNVCSPGTRLVYAGAPDTSHCIQSTAPTIDGDAWVTADVVVLGDERIVHYIDGVPVI
                     EYGGIAYGGGNVNGHDPKDKPDGSPLERGYISLQSESHPIQFRRVELLDLTGCTEACR
                     "
     gene            3225..>3767
                     /locus_tag="VFL84_00670"
     CDS             3225..>3767
                     /locus_tag="VFL84_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET7607161.1"
                     /translation="MRLKNLCLPHAGIVAAAAAFIGAAAAEPVRYELDPAHTTIAFLV
                     EHAGYASTLGQFLRTSGAYTFDDATGAVSDVRVVVETASVDTHHEARDRHLVSRDFLD
                     SGSHPEMTFVAASAQRTGESTFVVTGELTLLGKRRPLTLEATLNKSAPYPFGDRAPVM
                     GVSARAKLKRSEFGMTYGVAD"
BASE COUNT          576 a         1303 c         1318 g          570 t
ORIGIN      
        1 acgtccagga tgcggttcat gcgcttcaaa tcgacgacga cgctgcccgt gtaggccggc
       61 gcggcgccgc cataggttag attgcggccc gtcgagatcg gatagagcgg caaggcgtat
      121 cggttcgcga tcttgacgat ggcctggacc tgctcgacgg aattcggcgc gacggccgcc
      181 gaaggcaagc gctcctcggc ctcgccccag agcggcgaat agccgtcgcg gtagagcagc
      241 acgtcctcgt ccgtggcaaa tagccactcg cggccgacga cgccttcgag ctcgcgcagc
      301 gcggcttgga aatcgcgttc ggcgatgccg ggcggtggct taggcatgga aggcactccc
      361 gcggggatcg atcggcgcaa gcgcggcccg gcgcgcgggg ctcgaggata ggatccacgt
      421 gacggtcccg gccgcctctt cacggcgcac ggcgctcgcg ccgtagtcgc gcgccaggta
      481 ttcgaggcag aacgcggtcg cggcgctcgt gtagccctcg atgacgagcg cgcccgagcg
      541 cagccgcggc tcgagctcgc gcatccagag cgcggcaggg tcgcccgcga actcgaaagc
      601 agcgaggccg cgcgagcgcc tctcgaagag aaacgccgcg ctgccggcga gctgccgatc
      661 gacgacgacg attctcggcg caccgccggc gctcgtttcg gcgccgctcg cggaccaggt
      721 cgcgacggcg gcacccagtg cgacgctcga ttgcatgaag cggcggcggt cgatcatctt
      781 tctcccccgg ggcaagagtg tagccgcatg gctcaatgcg atagcgcgta gacggcgaaa
      841 ttgacgccga ggctgatcgc gaggttcgcg gcttcctgcg gatagtcgta ctcgtcggcg
      901 cgctgccagc cgtccgcgac gtcgttgttg aacgtgatca tgaccatgac gcgctcgtcg
      961 tcgtcgacga tcgcgcgcca ccgaggaatc gccccgtcgt tgcggtaacc gccgcgcggc
     1021 agatgctgcc aggtcgggat ctgcggccgc ttcgtcaagt cgtacacggc atggaagatc
     1081 ggatgcgtgt cgggcaactc gacgatcggc cgatcgggga acacgcgctg caggctcgcg
     1141 atgaaccctt cccattcgac ggtgccgtag aagctgtcgc agagcagaaa gccgccgcgc
     1201 agcagatact cgcgcagctt gagcgcctgc ccgtccgtga ggtcccaggc tccgacgagc
     1261 cccgagatca agaatggcca gtaatacgcg tcgatatcgt cgtcgagatt cacgggctgc
     1321 tcgacgagcc ggacgtcgat cgtcgtcaag cgcgcgagca ggcgcgcgaa ggtgcgatcg
     1381 ccctcgggat aatcgacggt ccacgccgtg ccgcccgccc gccagtcgcc ggggccggcg
     1441 ccgagcatgt agccgcggcg gatctgcgga aacatgagcc gcccgacgac gaactcgccg
     1501 ggcgcttcgt cgggatgggt gtcgcggccg taccagccgc gctcgatgct cgggtactcg
     1561 cgccaccttt cttgcgcgcc ggccgttgcg gcggcgagcg acaagacaga cgcgatggcg
     1621 aagtagagcc ggctcattca ccattactgt agcaagcggg gggcgcggag cgacatgacc
     1681 gtacaacgtc tctcgattcg ggggctcgcg gcggtccgga tcgtcgtcgc ggccgcgctc
     1741 gtggcgaggc tgcccgccgc ggccgacgag gtcgtcgtca tgacgtcggg ggcttttacc
     1801 gcaccgttcg aggtcgcggc cccctggttc gagcggcgca cgggccacga cgtcgtcgcg
     1861 gtgttcggcg cctcgacggg cggcgcgccc gactcgatcc ctgtgcggct cgcgcgcggc
     1921 gagcgcgccg acgtcgtcat cgcctcggcg caggccgtcg acgcgctgat cgcggcggga
     1981 gccgtcgagc gcggctcgcg ccgcgacctc gtgctgtcgc ggatcggcgt ggccgtgcgc
     2041 gccggggcgc gcaagcccga catctcctcg gtcgacgcgc tcgtccgagc ggtgcgcgag
     2101 gcgcgctcga tcgcgtactc ggcgagcgtc agcggcacgt atctcgcgac cgagctgttc
     2161 ccgcggctcg gcctcgccgc cgagatcgcg gccaagggcc gccgcatcga gagcgagcgc
     2221 gtcggcgccg tcgtcgcgcg cggcgacgcc gagctcggct tccagcagat cagcgagctg
     2281 gtcacgatcg acgggctcga gtacgtcggc cccttgcccg acgaggtcca gcgcgtcagc
     2341 acattctcgg ccggcatcgc ggccggcgcc aaaagcccgc ccgccgcgcg cgagctcgtc
     2401 gctttcctcg cgtcgatcgc gctcgctccc gtcgtcgagc gctacgggct cgatcaggtc
     2461 gagtacaccg cgagcgattg gcggccgctc ttcaacggcc gcgacctcgc ggcctggacg
     2521 ccgaagattc ggcggcatgc gctcggcgac aatttcgccg gcacgttccg cgtccgcgac
     2581 ggtatgatcg aggtcggcta cgaaggctac gatacgttcg ccgagcagtt cggccacctc
     2641 ttctatcgag agccgttctc gcgctaccgg ctgcgcgtcg agtatcgctt cgtcggcgcg
     2701 caggcgccgg acgcgccggc ctgggccgcg cgcaacagcg gcgtcatggt gcactcgcag
     2761 gcgcccgaga cgatgctgcg cgatcaggat tttccgatct cgatcgaggt gcagttcctc
     2821 gggggacttg gtgacggcaa ggcgcggccg acgggcaacg tttgctcgcc gggaacgcgc
     2881 ctcgtctacg ccggcgcgcc tgacacgtcg cattgcattc aatcgacggc gccgacgatc
     2941 gacggcgacg cgtgggtcac ggccgacgtg gtcgtgctcg gcgacgagcg catcgtgcac
     3001 tacatcgacg gcgtgcccgt catcgagtac ggcggcatcg cctacggcgg cggcaacgtc
     3061 aacggccacg atccgaaaga caagccggac ggctcgccgc tcgagcgggg ctacatctcg
     3121 ctgcaaagcg agagccatcc gatccagttt cggcgcgtcg agctgctcga cctcacgggc
     3181 tgcacggagg cttgccgcta gcaccgccct gtatcattgc cggcatgcga ctcaagaatc
     3241 tctgtctgcc ccatgccggc atcgtcgcgg ctgccgccgc cttcatcggc gccgcggccg
     3301 ccgagcccgt gcgatacgag ctcgaccccg cccatacgac gatcgcgttc ctcgtcgagc
     3361 acgccggcta cgcgagcacg ctcggccaat tcctgaggac gagcggggcc tacaccttcg
     3421 acgacgcgac gggcgcggtc tcggacgttc gcgtcgtcgt cgagacggcg agcgtcgaca
     3481 cgcatcatga ggcgcgcgat cgacacctcg tgagccgtga cttcctcgac agcgggtcgc
     3541 atccggagat gacgttcgtc gcggcgagcg cccagcgcac gggcgagagc acgttcgtcg
     3601 tgaccggcga gctcacgctg ctcggcaagc gcaggccgct gacgctcgag gcgacgctga
     3661 acaagagcgc gccctacccg ttcggcgatc gtgcaccggt aatgggcgtc tcggcgcgcg
     3721 cgaagttgaa gcgcagcgag ttcggcatga catacggcgt cgcggac
//
LOCUS       DASSNN010000037         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1143591, whole genome shotgun sequence.
ACCESSION   DASSNN010000037 DASSNN010000000
VERSION     DASSNN010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143591"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VFL84_00675"
     CDS             complement(<1..484)
                     /locus_tag="VFL84_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7607162.1"
                     /translation="MPLSRVRITTLRCLREVELELDPSRNYIFGPNGAGKTSLLEGLF
                     VLGRGRSFRTRQMRRLVRHGADGFAVFGAADVDGAERRLGVAYRAGRLEKKIDGQPAV
                     GMAQLAALLPVHAIDPSMHAIVEGGPSERRRFLDWGVFHVEPAYLDAWKSYRRVLSQR
                     N"
     gene            complement(515..1612)
                     /gene="dnaN"
                     /locus_tag="VFL84_00680"
     CDS             complement(515..1612)
                     /gene="dnaN"
                     /locus_tag="VFL84_00680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET7607163.1"
                     /translation="MKFSAARETLLKPLQTVVGVVERRQTMPILSNVLLSTQGGKLSI
                     TATDLEVEMVAEAEVKGDSGGDITVPGRKLHDIFRALPEGASVEFSLNGDRLTIKAGR
                     SRFTLSTLRAADFPTIDEIGAKQTLRLNKPDLRRLVEKTHFSMAQQDVRYYLNGLLLE
                     TDKKRLRAVATDGHRLALSEVELLGAASREEQLIVPRKGVLELSRLLEGDGEVQVALG
                     ANHIRVQLDGVRLTSKLIDGRFPEYSRVVPSQPKNTIKADRNLFRQALQRTAILSNEK
                     YRGVRLELSANNVVLQANNPEQEEAVETLEVEYAGDAMEIGFNVNYLLDALAAVDSEQ
                     VEFGVSDANSSCLIREPGSERTKFVVMPMRL"
     gene            complement(1798..3105)
                     /gene="dnaA"
                     /locus_tag="VFL84_00685"
     CDS             complement(1798..3105)
                     /gene="dnaA"
                     /locus_tag="VFL84_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET7607164.1"
                     /translation="MQCARALEAELSEQQFNTWIRPLQLVEDGPLLKLLAPNRFVVDW
                     VKTHCLEQIRTWWRRHVEGAPEILVEVGSRPAVRRDAPAAVPEPEVRPATTIGGRLNV
                     AFTFDSFVEGKSNQLARAAAFQVAQNPGSAYNPLFIYGGVGLGKTHLMHAIGNQILSR
                     NPAAKVAYVHSERFVSDMVRGLQHNTIAEFKRSYRSLDALLIDDIQFFAGKDRSQEEF
                     FHTFNALLEGQRQIVLTCDRYPKEVAGLEERLKSRFGWGLTVAIEQPELETSVAILMS
                     KAAAEGETLPEEVAFFLAQRIRSNVRELEGALRRVLANSRFTGQPITLEFAKDALRDV
                     LALQEKLVTVENIQKTVAEYFKIRVADLLSKRRSRSIARPRQIAMALAKELTTHSLPE
                     IGDAFGGRDHTTVLHACRRVKELRETETRVAEDYNNLLRTLTT"
BASE COUNT          536 a         1094 c         1022 g          627 t
ORIGIN      
        1 cgttgcgttg gctcagcacg cgccggtagc ttttccaggc gtccaggtac gctggttcca
       61 cgtggaacac tccccagtcc aggaaccgcc ggcgctcact gggaccgccc tcgacgatcg
      121 cgtgcatgct gggatcgatc gcgtgcacgg gcaatagcgc ggccagctgc gccatgccga
      181 cggccggctg gccgtcgatc ttcttctcga gccggccggc gcgatacgcg actccgagcc
      241 ggcgctccgc gccgtcgacg tcggccgcgc cgaacacggc aaagccgtcc gcgccatgcc
      301 ggacgagcct gcgcatctgc cgcgtccgaa acgaccgccc gcggccgagc acgaataggc
      361 cttcgagcag gctcgtcttg ccggcgccgt tcggtccgaa gatgtagttg cggctcggat
      421 cgagctcgag ctcgacctcg cgcaggcagc gcagcgtcgt gatgcggact cggctcagcg
      481 gcaagacagg tgcctccgag cgccgacggg cctgctagag gcgcatcggc atgacgacga
      541 acttcgtacg ctcgctgccc ggctcgcgga tcaggcaact cgagttcgca tcgctgacac
      601 cgaactcgac ttgctcgctg tcgacggccg ccagcgcgtc caacaggtag ttgacgttga
      661 agccgatctc catcgcgtcg ccggcgtact cgacctcgag cgtctcgacg gcttcctctt
      721 gctcggggtt gttcgcttgc agcacgacgt tgttcgcgct gagctcgagc cgcacgccgc
      781 gatacttctc gttcgagaga atcgccgtgc gctgcagcgc ctggcggaac agattgcggt
      841 cggccttgat cgtattcttc ggctggctcg gcactacgcg cgaatattcg gggaaacgac
      901 cgtcaatgag cttcgaagtg agtcgaactc cgtccaattg aacgcgaatg tgattcgcgc
      961 cgagcgcgac ctggacctcg ccgtcgcctt cgagcagccg gctcagctcg agcacgccct
     1021 tgcgcggcac gatcagctgc tcctcgcgcg acgcagcgcc cagcagctcg acctcggaca
     1081 gcgcgagccg atggccgtcg gtcgccacgg cgcgcaggcg tttcttgtcc gtctcgagca
     1141 gcaacccgtt caggtaatag cgaacgtcct gttgggccat cgaaaagtgc gttttctcta
     1201 ccaatcggcg cagatcgggc ttgtttagcc gcaaagtttg tttggcaccg atctcgtcga
     1261 tcgtcggaaa gtcggccgcg cgcagcgtcg acaacgtgaa gcggctgcgg cccgctttga
     1321 tcgtgagtcg atcgccattg aggctgaact cgactgacgc cccctccggc agcgcgcgga
     1381 aaatgtcgtg cagcttgcgg cccgggaccg tgatgtcccc tccgctgtcg cccttgacct
     1441 cggcctcggc gaccatctcc acctcgagat ccgtcgccgt gatgctgagc tttccgccct
     1501 gtgtgctgag cagcacgttg ctcaagatcg gcatggtttg ccgtcgttcc acgaccccaa
     1561 cgacggtctg caaaggcttc aggagggttt cccgcgctgc actaaacttc attgattgcc
     1621 tctacgtatc tatctgtttg ctgtagttct tagtgtccgc caaacgtgtt gttaaccggc
     1681 tttttctcta tgccgccagc gacttgcgct cgccgcaaca tgtgagtaac cgaacgccaa
     1741 acggtggttg gcctgtggag caatttcctg cgccgaccta cccaccgaca actcacgcta
     1801 tgtcgtcagc gttctcaaca ggttgttata gtcctccgcg actctcgtct ccgtctcgcg
     1861 cagctccttc actcgccggc acgcgtgcag cacggtcgta tggtcgcggc cgccgaacgc
     1921 atcgccgatc tcgggcaagc tgtgcgtcgt gagctctttc gcaagcgcca tcgcgatctg
     1981 gcgcgggcga gcgatcgagc ggctgcgccg tttcgacaat aagtccgcga ctctgatttt
     2041 gaaatactcg gcgacggtct tttggatgtt ctcgaccgtc acgagcttct cttgcagcgc
     2101 gagcacgtcg cgcagcgcgt ccttcgcgaa ctcgagcgtg atcggctgac ccgtgaaacg
     2161 cgagttcgcg agcacgcggc gcagcgctcc ctcgagctcg cgcacgttcg agcggatccg
     2221 ctgcgcgagg aagaacgcga cctcctccgg cagcgtctcg ccttcggcgg ccgccttgct
     2281 catcaggatc gcgacgctcg tctcgagctc gggctgctcg atcgcgaccg tgagacccca
     2341 accgaaccgc gacttcaacc gctcctcgag gcccgcgacc tccttcggat agcggtcgca
     2401 ggtcagcacg atctgccgtt gcccctcgag cagcgcgttg aacgtgtgga agaactcctc
     2461 ctgcgagcgg tccttccccg cgaagaactg gatgtcgtcg atcagcagcg catcgagcga
     2521 gcggtagctg cgtttgaact ccgcgatcgt gttgtgctgc agcccgcgca ccatgtcgct
     2581 gacgaaccgc tccgaatgca cgtacgcgac cttcgcggcg gggttgcgcg agagaatctg
     2641 attgccgatc gcgtgcatca agtgcgtctt gccgagaccg acgccgccgt agatgaagag
     2701 cgggttatag gcgctgccgg gattctgcgc gacctgaaac gcggccgcgc gcgcgagctg
     2761 gttgctcttg ccctcgacga agctgtcgaa cgtgaaggcg acgttgagcc gcccgccgat
     2821 cgtcgtggcg gggcgtacct cgggctcggg gaccgcggcc ggcgcgtcgc ggcgcacggc
     2881 gggacggctg ccgacctcga ccaagatctc gggcgcgccc tcgacgtgcc ggcgccacca
     2941 ggttcgaatc tgctcgaggc agtgcgtctt gacccagtcg acgacgaagc ggttcggcgc
     3001 cagcagcttc agcagcggcc catcctcgac gagctgcagc ggccgaatcc acgtgttgaa
     3061 ctgctgctcc gatagctcgg cttcgagcgc gcgtgcgcac tgcatccaaa gagacgagta
     3121 aaccgccgag ttctctgcca cgggagctcg cgaaaaaagt tggttgcggg acggccaggg
     3181 agtggggggc cagtctaaga aagttcgccg tggttatcca caaccatcga cgacatcgcg
     3241 tgcgagcgct tcgcgtttta gtggcggctc ttcgcggtt
//
LOCUS       DASSNN010000038         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_513010, whole genome shotgun sequence.
ACCESSION   DASSNN010000038 DASSNN010000000
VERSION     DASSNN010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513010"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VFL84_00690"
     CDS             complement(<1..1176)
                     /locus_tag="VFL84_00690"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET7607165.1"
                     /translation="MKVNKVHAAVVAALTSATGLTSTAAFAQEALEEIVVTATRREQN
                     LQDVPVSIVAVTGENLTVRGIDTLEELSQAIPNVVATGGGGGTAGTGFSVRGIPNVGT
                     YVDGVWQIDTAGALVREFVDLDRIEVLRGPQGTRFGRDSTGGAIRIWTKRPTEEFGGN
                     VTAVAGSLDRRDVKATVDVPFSDNVLSKWTAASLSRDGYIENLTTGEKDGFVDQTIFR
                     GDIVWNATDKLSMRFNYLYNDNTFTEPKVVDAIFRTYGDPGIMDVIGLPEFYSQVPGV
                     EPFSAANNQAGFPGGKVGQWQNRSNMTVANNMEAEQVMVDINWDFGETMSVEFLTAYT
                     DQYNKLANDWDGSQYDIVYDLNIEDNELWSQEIQFSGGKGRVSWLGGAYYWEQSTLQR
                     "
     gene            complement(1441..>2382)
                     /locus_tag="VFL84_00695"
     CDS             complement(1441..>2382)
                     /locus_tag="VFL84_00695"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET7607166.1"
                     /translation="LKRRMSIEFHRRMLADRVRHEAFRAALKKAIEPGKSAVADIGAG
                     LGVLAFFARELGAREVWLYDPGPALELAEVVAARNGIEGLHFVRERSLDVADPPLVDV
                     VVAEVLGNFAYEEDVLETLRDAQRFLRPGGTLIPASIEQWVAPVTADRFERDLSSWRE
                     VGFGLDWSDAERMSRNNMYVFPIEPKDLLADAAQRWDSLDFHGAIESRRSGRAAWRMR
                     EARPLYGFALWWDCTLAPGVVLSTSPHAPRTHWDQIYLPLLTPIAAEPGDEITVTIES
                     ETGGGETGIEVRWTVRHARGGRPADEQALSIGSGWLA"
BASE COUNT          406 a          798 c          746 g          432 t
ORIGIN      
        1 ccgctggagg gtgctttgct cccaatagta ggcgccgccg agccagctca cgcgtccttt
       61 gccgcccgaa aactggatct cttggctcca gagctcgttg tcctcgatgt tcaggtcgta
      121 gacgatgtcg tactggctgc cgtcccagtc gttcgcgagc ttgttgtact ggtcggtgta
      181 cgccgtcaag aactccacgc tcatggtctc gccgaagtcc cagttgatgt cgaccatcac
      241 ctgttcggct tccatgttgt tggccacggt catgttcgag cggttctgcc actgaccgac
      301 cttgccgccc gggaagcccg cctggttgtt cgcggcgctg aagggttcga cgcccggcac
      361 ctggctgtag aattccggca agccgatcac gtccatgatt ccggggtcgc cgtacgtgcg
      421 gaaaatcgcg tcgacgactt tcggctcggt gaacgtgttg tcgttgtaga ggtagttgaa
      481 ccgcatcgac agcttgtcgg tggcattcca cacgatgtcg ccgcggaaga tcgtctggtc
      541 gacgaagccg tctttctcgc cggtcgtcag gttctcgata tagccgtcgc gggataggct
      601 cgcggccgtc catttcgaga gcacgttgtc gctgaacggc acgtcgacgg tcgccttgac
      661 gtcgcgccga tcgagcgagc cggccaccgc ggtgacgttg ccgccgaact cctcggtggg
      721 gcgcttcgtc cagattcgga tcgcgccacc ggtcgagtcg cggccgaacc gcgtgccttg
      781 cggaccacgc agcacctcga tgcgatcgag gtcgacgaac tcgcgcacga gcgcgccggc
      841 cgtatcgatc tgccaaaccc cgtcgacata ggtgccgacg ttcggaatgc cgcgaacgct
      901 gaagcccgtg cccgccgtgc cgccgccgcc gcccgtggcg acgacgttcg gaattgcttg
      961 gctcagctcc tcgagcgtat cgatgccgcg aacggtgaga ttctcaccgg tgactgcgac
     1021 gatcgaaacc ggaacatctt gaagattctg ctcacggcgt gtggccgtga cgacgatctc
     1081 ctcgagagct tcttgagcga aagcggccgt agacgtcaga ccggtcgcgc tcgtcagtgc
     1141 tgctacgaca gcagcgtgga ctttgttgac cttcataagc atcccccaaa ttggtttcgt
     1201 gaaccggccg aaacgttgtc gccacgaccg cctagcaaac ttgcgaccga gccgcggacc
     1261 ccggtctcaa tccaacagcg aaaacatgag atttgtgacc gccgagtctg caaaaatccg
     1321 cgtccagggt caacggggac gcgttgcttt tgcccgactg ttattggtaa taccaatcaa
     1381 atcgacgcga ccgccgcgaa ttccgcgccc aaccgctgtt ggatcggggc aacggccggt
     1441 tcaggcgagc cagcccgagc cgatcgacag tgcctgctcg tcggccgggc ggcccccgcg
     1501 cgcgtggcgg acggtccagc gcacctcgat accggtctcg ccgccgcccg tctcgctttc
     1561 gatcgtgacc gtaatctcgt ccccgggctc ggccgcgatc ggcgtcagca ggggcagata
     1621 gatctggtcc caatgcgtcc tgggcgcgtg cgggctcgtc gacagcacga cgccgggcgc
     1681 gagcgtgcag tcccaccaga gcgcgaagcc gtacaacggc cgcgcctcgc gcatccgcca
     1741 cgcggcccgg ccactgcggc ggctctcgat cgcgccgtga aaatcgagcg agtcccacct
     1801 ctgcgccgcg tcggcaagca ggtctttcgg ctcgatcggg aacacgtaca tgttgttgcg
     1861 cgacattcgc tcggcatccg accaatcgag accgaagccg acctcgcgcc agctcgaaag
     1921 gtcgcgctcg aagcggtcgg ccgtgaccgg cgcgacccac tgctcgatcg acgcggggat
     1981 cagcgtgccg ccgggccgca agaagcgctg cgcgtcgcgc agcgtctcga gcacgtcctc
     2041 ttcgtaggca aaattgccga gcacttccgc gacgacgacg tcgacgagcg gcggatcggc
     2101 gacgtcgagc gagcgctccc gcacgaagtg cagaccttcg atgccgttgc gcgcggcgac
     2161 tacctccgca agctcgagcg cgggcccggg gtcgtacagc caaacttcgc gcgcgccgag
     2221 ctcgcgcgcg aagaacgcga gcacacccaa gccggcgccg atgtcggcga cggcgctctt
     2281 accgggctcg atcgcttttt tcaaagccgc gcgaaacgcc tcgtggcgaa cgcggtctgc
     2341 cagcatgcgt cgatggaatt cgatcgacat gcgccgctta ag
//
LOCUS       DASSNN010000039         4245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_136012, whole genome shotgun sequence.
ACCESSION   DASSNN010000039 DASSNN010000000
VERSION     DASSNN010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136012"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VFL84_00700"
     CDS             <1..521
                     /locus_tag="VFL84_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET7607167.1"
                     /translation="GSLLLLRTGAQTRDEDVGYRNSQIYFSGDYYVRNLGRHLFSVSL
                     DALATDELDPETQVLLGGDNGLRGYPIRYQAGENSVVLNIEQRFFTDFYPWRLIRVGW
                     AVFADIGKVTGRDPRATEPLGTLYDVGAGLRLSSPRAAGRQVLHVDLAFPLKRHPDID
                     SVQFVIETKRSF"
     gene            complement(612..1223)
                     /locus_tag="VFL84_00705"
     CDS             complement(612..1223)
                     /locus_tag="VFL84_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7607168.1"
                     /translation="MIRVHATKASINGYKVRLLLALLRQPYELVELDMYAGEHKREPF
                     LTLNPFGQMPALTDGAFTIADSHACLVYLARKYDASGTWLPADAEGEAKVAEWLSKSA
                     NEVHQGPWMKRAKIRRPDAIKLSNEEIDARCDHILKLMDAWLAKRDWLALDRATIADI
                     SCFGPISMLKVSGYDTDQWPNVTRWLERVKALPGAHDIDGNPF"
     gene            1274..2185
                     /locus_tag="VFL84_00710"
     CDS             1274..2185
                     /locus_tag="VFL84_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7607169.1"
                     /translation="MKPTTTATAQIHFCVVLWGFTAILGKLISLAALPLVWWRMLIVV
                     AALAFVPSVLRSLRALPARLWLAYAGVGGLVALHWLTFYGAIKLANASVAATCIALAA
                     PLTALVEPWLTRSRFSARELALGLAVLPGVALVVGGVPAGMREGVVVGAVSALLVAVF
                     GSLNKRLAHRGEALTATALELGAGLVVLSALAPLLPMVVPKLAGPLLEWPTPRDGILL
                     ITLALACTLLPFSLSFVALRHISAFSAQLAINLEPVYAIVLAALLLGEQRELTVQFYV
                     GVAIILAGVSLQPLFGRAREVGVSSGA"
     gene            2238..2762
                     /locus_tag="VFL84_00715"
     CDS             2238..2762
                     /locus_tag="VFL84_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7607170.1"
                     /translation="MHESSRSFPAEGGCDCRAVRYRMQTAPLFVHCCHCRWCQRESGA
                     SFALNAMIEADRVVELGQPAELVRTPSASGSGQLVARCSTCGIAVWSHYAGAGPVIKF
                     VRVGTLDDPDLLPPDIHIFTSSKQPWVVLPEGIPAVPEYYDRDEHWPEESKARRIAIL
                     PLIEAYKASRRGRG"
     gene            complement(2763..3986)
                     /locus_tag="VFL84_00720"
     CDS             complement(2763..3986)
                     /locus_tag="VFL84_00720"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7607171.1"
                     /translation="MIIAFARAAHGADASALFADGERAFAAGEYAEALRLFTAAREAG
                     STGPSSYYNIGVCQYRLRQFDAAEATFAMLADEFPAMRELAEYNRGLALRAGGNLAGA
                     RVAFERARQSTDEKIVALANGQLADLGPRVVADEARWTGYFASALGFDDNVALLNELV
                     LASSEASSPLAEVLGVVTRDFGSRPLRFDASGYAVHYPDVGDFDQTALRLSLEGELSF
                     GRWSLFVGPTLGRTTLNGEGFEELAGADMRLRRGFGDGFVFEARAVYDDTSAADSQFA
                     YLDGWHRLLRLSVQHTGTARVRAAYDVERDDRADPGVSPSRERLGIFYQRRLSQTWSA
                     DATAAYRTSRYSAASVPREEQLLELTFAARRTLGREWTLGVEYQWFNNDSTVEDFAYD
                     GQRFALGLSRSFYGP"
BASE COUNT          628 a         1486 c         1399 g          732 t
ORIGIN      
        1 cgggcagcct gttgctgctc cgaacgggcg cccaaacgag agatgaggat gtaggctacc
       61 ggaactccca gatctatttc tccggcgact actatgtgcg caatctcgga agacacctat
      121 tctcggtcag cttggatgcg ctcgcgaccg acgagctgga ccccgagacg caagtcctgc
      181 tcggcggcga caacgggctc cgcggctacc ctattcgcta ccaagccggc gagaacagcg
      241 tggtcctcaa catcgagcag cggttcttca cggacttcta cccgtggcgc ctcattcgcg
      301 tcggctgggc cgtgttcgcc gacatcggca aggtcacggg ccgcgatccg cgcgcgaccg
      361 agccgcttgg gacactctac gacgtcggcg cgggcctgcg cctgagctcg ccgcgcgcgg
      421 ccgggcgcca agtcttacac gtcgacctcg cgtttccgct gaagcgccac cccgacatcg
      481 acagcgtgca gttcgtcatc gagacgaaac gctcgttctg aggtgccggt gttacggttt
      541 cggttcaacc gcaactccgg cacttcggtt gaaccgaaac cgtgacaccg gcacctcaga
      601 aggccggcac ctcagaaggg gttgccgtcg atgtcgtgcg cgccgggcaa cgccttgacg
      661 cgctcgagcc agcgcgtcac gttcggccat tggtcggtgt cgtagcccga caccttcagc
      721 atcgagattg ggccgaagca cgagatgtcg gcgatcgtcg cgcggtcgag cgcgagccag
      781 tcgcgttttg cgagccacgc gtccatgagc ttcaagatgt ggtcgcagcg cgcgtcgatc
      841 tcctcgttcg agagcttgat cgcgtcgggc cggcgaatct tcgcgcgctt catccacggc
      901 ccctggtgaa cctcgttcgc cgacttcgac aaccattcgg caaccttcgc ctctccctcg
      961 gcgtcggccg gcaaccacgt cccgctcgcg tcgtacttgc gagcgagata cacgagacac
     1021 gcatgcgaat ccgcgatcgt gaacgcgcca tccgtcagcg ccggcatttg cccgaacggg
     1081 ttcagcgtta ggaacggctc gcgcttgtgc tcgccggcgt acatgtcgag ctcgacgagc
     1141 tcgtacggct gacgcagcag cgcgagcagc agccgcacct tgtagccgtt gatcgacgct
     1201 ttggtcgcgt gcacgcgaat catcgtttgc tctcctatgg cggcgcccag tcgctctaat
     1261 atagcgagac gccatgaagc ccacgaccac cgcgaccgcg caaatccact tctgcgtcgt
     1321 gctgtgggga ttcacggcga tcctcggcaa gctgatctcg ctcgccgcgc tgccgctcgt
     1381 gtggtggcgc atgctgatcg tcgtggccgc gctcgcgttc gtgccgagcg tgctgcgctc
     1441 gctgcgcgcg ttgccggcgc ggctctggct cgcctacgcc ggcgtcggcg gactcgtcgc
     1501 gctgcactgg ctcacgttct acggcgcgat caagctggcg aacgcctccg tcgccgcgac
     1561 ctgcatcgcg ctcgcggcgc cgctcacggc gctcgtggag ccgtggctca cgcgcagccg
     1621 cttctccgcg cgcgagctcg cgctcgggct ggccgtgctg cccggcgtcg cgctcgtcgt
     1681 cggcggcgtt ccggccggca tgcgcgaggg cgtcgtcgtc ggcgcggtgt cggcgctgct
     1741 cgtcgccgtg tttggctcgc tcaacaaacg gctcgcgcat cgcggcgagg cgctgacggc
     1801 gacggcgctc gagctcggcg cgggcctcgt cgtgctctcg gcgctcgcgc cgctcttgcc
     1861 catggtcgtt cccaagctcg cggggccgct gctcgaatgg ccgacgccgc gtgacggcat
     1921 cttgctgatc acgctcgcgc tcgcgtgcac gctgctgccg ttctcgctgt cgttcgtggc
     1981 gctgcggcac atcagcgcgt tctcggcgca gctagcgatc aacctcgagc cggtctacgc
     2041 aatcgtgctc gcggcgctgc tgctcggcga gcagcgtgag ctcaccgtgc agttctacgt
     2101 cggcgtggcg atcattctcg cgggcgtgtc gttgcagccg ctgttcggcc gggcgcgcga
     2161 ggtcggcgtc tcttccggcg cttgaacgtt gacgctaccg ggcgcgcggt gcttagctcg
     2221 gcgcatcgga gagcgacatg cacgagagct cgagatcctt cccggccgaa ggcggctgcg
     2281 actgccgcgc cgtgcgctac cgcatgcaga ccgcgccgct gttcgttcac tgctgtcact
     2341 gccgctggtg tcagcgcgag tccggcgcgt cgttcgcgct gaacgcgatg atcgaggccg
     2401 accgcgtcgt ggagctcggc caaccggccg agctcgtgcg gacgccgtcg gccagcggct
     2461 ccggtcagct cgtcgcgcgc tgctccacgt gcggaatcgc ggtctggagc cattacgcgg
     2521 gcgccggccc cgtgatcaag ttcgtgcggg tcggcacgtt ggacgatccc gacttgctgc
     2581 ctccggacat tcatatcttc acgagctcga agcagccgtg ggtcgtgctg ccggaaggaa
     2641 tacccgccgt ccccgagtac tacgaccgcg acgagcactg gcccgaggag agcaaagcgc
     2701 ggcgcatcgc gatcctgccg ctgatcgagg cctacaaagc ctcgcggcgc ggccgcggtt
     2761 agctacggac cgtagaagct tctgctcaag ccgagcgcga accgctgccc gtcgtaggcg
     2821 aaatcttcga ccgtcgagtc gttgttgaac cattggtatt cgacgccgag cgtccactcg
     2881 cggccgagcg tgcgccgcgc ggcgaacgtg agctcgagca gctgctcctc gcgcggcacg
     2941 ctcgccgcgc tgtaccggct cgtgcggtac gcggctgtcg catccgcgct ccacgtctgc
     3001 gatagccgcc gctgatagaa gatgcccaac cgctcccgcg acggcgacac gccgggatcg
     3061 gcgcggtcgt cgcgctcgac gtcgtacgca gcgcgaacac gagccgtgcc cgtgtgctgc
     3121 accgacaaac gcagcagccg gtgccaaccg tccaaatacg cgaactgcga atcggctgcg
     3181 ctcgtgtcgt cgtagacggc gcgcgcttcg aaaacgaagc cgtcgccgaa gccgcgccgc
     3241 agccgcatgt cggcgcccgc gagctcctcg aatccttcgc cgttcagcgt cgtgcgcccg
     3301 agcgtggggc cgacgaagag gctccatcgc ccgaacgaga gctcgccctc gagcgacaga
     3361 cgaagcgcgg tctggtcgaa gtcgccgacg tcgggatagt gcacggcgta gccgctcgca
     3421 tcgaagcgca acggtctcga tccgaagtcg cgcgtcacga cgccgagcac ttcggcgagc
     3481 gggctcgatg cttcactgct cgcgagcacg agctcgttca agagcgccac gttgtcgtcg
     3541 aagccgagcg cactcgcgaa atatccggtc cacctcgcct cgtcggcgac gacgcgtgga
     3601 ccgaggtcgg caagctgccc gttcgcgagc gccacgattt tctcgtccgt gctctggcgc
     3661 gcgcgctcga acgcgacgcg ggcgccggcg agattgccgc ctgcccgcag cgcgaggccg
     3721 cggttgtact ccgcgagctc gcgcatcgcc ggaaactcat cagccagcat cgcgaacgtc
     3781 gcctcggccg cgtcgaattg ccgaagccgg tactggcaga cgccgatgtt gtagtagctg
     3841 ctggggcccg tggaacctgc ttcgcgagcg gccgtgaaca gccgcagcgc ttccgcgtac
     3901 tcaccggccg cgaacgcgcg ctcgccgtcc gcaaacaagg cgctcgcgtc ggcgccgtga
     3961 gccgcacgag cgaacgcgat gatcacgcag accgttgcga agagcgaagc ccgaaacatc
     4021 accgaccgta gaaaaagctg tgtcgccggt agtgtacaag gcactaccgc ggcgcttcag
     4081 actgggctaa aggctatgga cgcccgggcg gtgtttccgg tggtcctcgc gggttgtcgg
     4141 gcgggcccgg agtctcgggc ggcccgcgcc ccggcgcgtc atccggtcgt ccggcattct
     4201 cgcggttccc acgcgcctgc tctgcggttt cttgaccgag ctcgc
//
LOCUS       DASSNN010000040         4950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_136189, whole genome shotgun sequence.
ACCESSION   DASSNN010000040 DASSNN010000000
VERSION     DASSNN010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136189"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFL84_00725"
     CDS             complement(<1..188)
                     /locus_tag="VFL84_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HET7607172.1"
                     /translation="MQNNVAVRNIDRADPSVVKELGEHGVATVHEAQGRTGLLHPYLR
                     PIYSGARIGGSAVTVLLHP"
     gene            276..1448
                     /locus_tag="VFL84_00730"
     CDS             276..1448
                     /locus_tag="VFL84_00730"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7607173.1"
                     /translation="MTDSAPLTGIKVVDTTEALAGPYCAMLLGDLGADVIKVERPGDG
                     DQARGWGPPFLEGESAYFLSTNRNKRSIELDTKNAADARVLETLIGRADVFLTNNPRL
                     ESLARVSLDPKTLRARHPRLVYGAISGYGHTGPKANRGGYDIIAQGEAGLMALTGPVD
                     GGPTRFPSPMADISGGIYTCMGVLAALYARDNRASGTGMGQLIDVALVDAQLSWLANI
                     GASYFATGKRPARLGNAHPNVTPYQPVRARDKIMIIAVGNERLWAKFCKILGAESTLM
                     ADARFATNAERSAHRVELIDEIEKILVRRTAAEWVEELVAAGIPAGPINFPDEALTDE
                     HVVARRMIVELEHPLIGVVRSIAVPMRFESNGPTYRRYPPRLGEHNAEIRALAATW"
     gene            complement(1500..2372)
                     /gene="galU"
                     /locus_tag="VFL84_00735"
     CDS             complement(1500..2372)
                     /gene="galU"
                     /locus_tag="VFL84_00735"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET7607174.1"
                     /translation="MPGIRTAVFPVAGRGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     ALDAGAERLVFITGSSKRAIEDHFDTDAELERQLAESGKHDLLALVRDIIPPEATCIF
                     VRQGVPLGLGHAVLCAKHAVGNEPFFVHLADDLIYGEVGCLKQMRMHFDKHGTSVLGV
                     ETVPRDQTGSYGIVAVQKEPSGAQRVVKIVEKPKPADAPSNLAVVGRYILTPAIFGKL
                     ERTQRGAGGEIQLTDGIAALLADEPVHVLPFVGTRYDCGSKLGYLRAMVEYGLRHPEL
                     GDSFRAYLENVRER"
     gene            complement(2518..2802)
                     /locus_tag="VFL84_00740"
     CDS             complement(2518..2802)
                     /locus_tag="VFL84_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7607175.1"
                     /translation="MRSILFRLVPALAFGCAFAAAAGPVNINTADAAKLASELQGVGP
                     ALAEAIVADRKANGTFATPEALMRVKGIGARIVEINKANILVADPAPPAR"
     gene            complement(2897..4081)
                     /locus_tag="VFL84_00745"
     CDS             complement(2897..4081)
                     /locus_tag="VFL84_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7607176.1"
                     /translation="MPTDATFLLAGLFIALGAIVYIYKLYTDRDRDLPPQISADYLRG
                     LNLVLNRRTDEALELFVQMAKVDDDTLETHFALGHLFRRRGEMERAIRVHENLLARPN
                     LNEAQRHQALFSLAEDYLGAGLFDRAEQLFSQLRDSTTLAEAALEKLVSIYEREQEWQ
                     KAIEALRKLEILRGSKSSQVAHYYCELAEIARAAGDRALAREHLRNTVRSESGALRGT
                     LIRATLAREEQDYAQAIPLYEQVIAADRHFISEVLPPLLDCYRAAGRLREFDAYVDEL
                     IAKERSAYRDVAYAAIIGDLRGSNALEGAVERFVFEHPVLANLVNAAELRALPAAERA
                     AAIQRIAGSLRSIAMANARYRCSNCGYSTQRFIWHCPSCKLWETVRPIQSVPLENVLA
                     RP"
     gene            complement(4088..4345)
                     /locus_tag="VFL84_00750"
     CDS             complement(4088..4345)
                     /locus_tag="VFL84_00750"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HET7607177.1"
                     /translation="MLVALVLLTGVFAYSNPQPIDVDIGVVRFEQVSMAVAFAVALAV
                     GFVLGVLSAGLTLWRSAGEKRRLEQDLKYAEAELRARQPPS"
     gene            complement(4472..4777)
                     /locus_tag="VFL84_00755"
     CDS             complement(4472..4777)
                     /locus_tag="VFL84_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HET7607178.1"
                     /translation="MMTKSELIESIARSQKHLPAKDVELAVKHILELMSEALANGERI
                     EIRGFGSFSLHYRPPRMGRNPKTGEAVALAGKNVPHFKPGKDLRERVNESRHLPVRD"
BASE COUNT          825 a         1642 c         1647 g          836 t
ORIGIN      
        1 ggatgcagca gcaccgtgac cgcgctgccg ccgatgcgcg cgcccgagta gatcggccgc
       61 aggtacgggt gcaacaggcc ggtgcggccc tgcgcctcgt gcaccgtcgc gacgccgtgc
      121 tcgccgagct ccttgacgac actcgggtcg gcacgatcga tgttgcggac cgcgacgttg
      181 ttctgcacga cactctctcc tgtcactcgt gaaaagaccg gcgcttatac tagccgccga
      241 tcagctaccg atcattacag cacaccggag aggccgtgac cgacagcgcc ccgctgacag
      301 gaatcaaagt cgtcgacacg acggaggcgc tcgcgggccc gtactgcgcg atgctgctcg
      361 gcgatctcgg cgcggacgtg atcaaggtcg agcgccccgg cgacggcgat caggcgcgcg
      421 gctggggacc gccgttcctc gaaggcgaga gcgcgtactt tctctcgacg aatcgcaaca
      481 agcgcagcat cgagctcgac acgaagaacg ccgccgacgc gcgcgtgctc gagacgctga
      541 tcggccgcgc cgacgtgttt ctcacgaaca atccgcggct cgagtcgctc gcgcgcgtga
      601 gcctcgaccc gaagacgctg cgcgcgcgcc atccgcggct cgtctacggc gcgatcagcg
      661 gctacgggca cacggggccg aaggcgaatc gcggcggtta cgacatcatc gcgcaagggg
      721 aggccggcct gatggcgctg accgggcccg tcgacggcgg cccgacgcgg tttccgtcgc
      781 cgatggccga tatctcgggc ggcatctaca cctgcatggg agtgctggcg gctctgtacg
      841 cgcgcgacaa tcgcgcgagc ggaaccggca tggggcagct catcgacgtc gcgctcgtcg
      901 acgcgcagct ctcgtggctc gcgaacattg gcgcgagcta cttcgcgacg ggcaagcgcc
      961 cggcgcgcct cggcaacgcg catccgaacg tcacgccgta tcaacccgtg cgcgcgcgcg
     1021 acaagatcat gatcatcgcc gtcggcaacg agcggctgtg ggcgaagttc tgcaagatcc
     1081 tcggcgccga gtcgacgctg atggcggacg cgcgattcgc gacgaacgcc gagcgcagcg
     1141 cgcaccgcgt ggagctcatc gacgagatcg agaagatcct cgtgcgccgc acggccgccg
     1201 aatgggtcga ggagctcgtc gccgcgggca ttccggccgg gccgatcaat tttcccgatg
     1261 aggcgttgac cgacgagcac gtcgtcgcgc ggcgcatgat cgtcgagctc gagcatccat
     1321 tgatcggcgt cgtgcggtcg atcgccgtgc cgatgcggtt cgagtcgaat ggcccgacgt
     1381 atcgccgcta tccgccgcgc ctcggcgagc acaacgcgga aatccgcgcg ctggctgcaa
     1441 cttggtgaga gctaaagggg acagatctat tttccgaaaa tagatctgtc cccttttcgc
     1501 tagcgctcgc ggacgttttc gaggtaggcg cggaagctgt cgccgagctc cgggtgacgc
     1561 aggccgtatt cgaccatcgc gcgcagatag ccgagcttgc tgccgcagtc gtagcgcgtg
     1621 cccacgaacg gcagcacgtg cacaggctcg tcggcgagca acgccgcgat gccgtccgtc
     1681 aattgaatct cgccgccggc gccgcgttgc gtgcgctcga gcttgccgaa gatggccggc
     1741 gtcagaatgt agcggccgac gacggccaag ttcgacggcg cgtccgccgg cttcggcttc
     1801 tcgacgatct tgacgacgcg ttgtgccccg ctcggctcct tctgcacggc cacgatgccg
     1861 tagctgccgg tttgatcgcg cggcaccgtc tcgacgccga gcacgctggt tccgtgcttg
     1921 tcgaaatgca tgcgcatctg cttgaggcac cccacttcgc catagatgag gtcgtcggcg
     1981 aggtgcacga agaacggttc gttgccgacc gcgtgcttcg cgcagagcac ggcgtggccg
     2041 agcccgagcg gcacgccctg gcggacgaag atgcaggtgg cctcgggcgg gatgatgtcg
     2101 cggacgagcg ccaacaagtc gtgcttgccg gactccgcga gctggcgctc gagctccgcg
     2161 tccgtgtcga agtgatcttc gatcgcgcgc ttcgacgagc ccgtgatgaa gacgaggcgc
     2221 tcggcgccgg cgtcgagcgc ttcctcgacg gcgtactgga tcagcggttt gtcgacgatc
     2281 ggcagcatct ctttcggaga cgccttcgtc gccggcagga accgcgtccc gcgacccgcg
     2341 acagggaaca ctgcagtccg aatgcctggc atcgatatct ctctcaaaac tcttcacttg
     2401 agatgatacc taacgccacg tgatcacgtg cggcggtgtt cacaatcgcg ccaaaacgga
     2461 cgcgggaccc gtaggccccg cgcccgcttt tggagcgcac gaacggtcgg cgaccggtca
     2521 gcgggcgggc ggcgccggat ccgcgacgag aatgttcgct ttgttgatct cgacgatgcg
     2581 cgcgccgatg cccttgacgc gcatcaacgc ctcgggcgtc gcaaaggtgc cgttcgcttt
     2641 gcggtctgcg acgatcgcct cggcgagcgc cgggccgacg ccttgcagct cgctcgcgag
     2701 cttcgccgcg tcggccgtgt tgatgttgac gggacccgcc gccgcggcga acgcgcaacc
     2761 gaacgcgagc gcggggacga gtcggaacag gatcgatctc atgagcatgc tcctatggtt
     2821 gttaaggagc ggccatgcta gtcggcgccg actgcgctag cagtgcggca aactgggctg
     2881 atcgagcagt ttttcgttac ggccgcgcga gcacgttctc gagcggcacg ctctggatcg
     2941 gacggaccgt ttcccacagc ttgcagctcg ggcaatgcca gatgaatcgc tgcgtgctgt
     3001 aaccgcagtt cgagcagcga tagcgagcgt tcgccatcgc gatgctgcgc aagctgccgg
     3061 cgatccgctg gatcgcggcc gcgcgctccg ccgctggcaa agcgcgcagc tcggcggcgt
     3121 tgacgagatt tgcgagaacc ggatgctcga acacgaaccg ctcgacggcg ccttcgagcg
     3181 cgttcgagcc gcgcaagtcg ccgatgatcg ccgcgtacgc gacgtcgcgg tacgccgaac
     3241 gctccttcgc gatgagctcg tcgacgtagg cgtcgaactc gcgcagccgg cccgccgcgc
     3301 gatagcagtc gagtagcggc ggcaacacct cgctgatgaa gtggcggtca gccgcgatga
     3361 cttgctcgta gagcgggatc gcttgcgcgt agtcctgctc ctcgcgcgcc aacgtcgcgc
     3421 ggatcaacgt gccgcgcagg gcgccggact cgctgcgcac cgtgttgcgc aggtgctcgc
     3481 gcgcgagcgc gcgatcgccg gccgcgcgcg cgatctcggc gagctcgcaa tagtaatgag
     3541 cgacctggct cgacttgctg ccgcgcaata tctcgagctt gcgcagtgcc tcgatcgcct
     3601 tctgccattc ttgctcgcgc tcgtagatgc tgacgagctt ttcgagcgcg gcctcggcga
     3661 gcgtcgtgct gtcgcgcagt tgactgaaca gctgctcggc tcgatcgaac aagcccgcgc
     3721 ccaagtagtc ctcggcgagc gagaacagcg cctgatgccg ttgcgcctcg ttcaagttcg
     3781 gacgcgccaa caggttctcg tggacgcgga tcgcgcgctc catctcgccg cgccggcgga
     3841 acaggtggcc gagcgcaaaa tgcgtctcga gcgtgtcgtc gtcgaccttc gccatctgca
     3901 cgaacagctc gagcgcctcg tcggtgcggc ggttcagcac gaggttcaac ccgcgcagat
     3961 agtccgcgct gatctgcggc ggcaggtccc ggtcgcggtc ggtgtagagc ttatagatgt
     4021 agacgatcgc accgagggcg atgaacagtc cggcgagcag gaacgtggcg tcggtaggca
     4081 tcgtgcttta agacggcggc tgccgtgcgc gcagctcggc ctcggcgtac ttgaggtcct
     4141 gctcgaggcg gcgcttctcg cccgccgagc gccagagcgt caggccggcc gacagcacgc
     4201 cgagcacgaa accgacggcc agcgcgacgg cgaacgcgac ggccatcgag acctgctcga
     4261 aacgcacgac cccgatgtcg acgtcgatcg gctgcggatt gctgtacgcg aacacgcccg
     4321 tcagcagcac caacgcaacc aacacggtgg cgaatacgag gcggcgcact tcaagaggtg
     4381 ccggtgttac ggtttcggtt caaccgaagc aatggtaccg gtgtccttgg ttcggctgaa
     4441 ccgaaccgta acaccggcac caccggagcg gctagtcgcg cacgggcagg tgccgggact
     4501 cgttgacgcg ctcgcggagg tccttgccgg gcttgaagtg agggacgttc ttgcccgcca
     4561 gcgccaccgc ctcgccggtc ttggggttgc gccccatgcg cggcgggcgg tagtgcagcg
     4621 agaacgagcc gaagccgcga atctcgattc gctctccgtt ggccagcgct tcgctcatga
     4681 gctcgaggat gtgcttgacc gccagctcca cgtccttcgc gggcaaatgc ttctgactgc
     4741 gcgcgattga ctcgatcagc tcggattttg tcatcagtac ggaccctata gctcgaccac
     4801 ggcactcccc gcgctttgga cgcgaagacc tacctcaacc tcattcggga cccatttgct
     4861 ccttgagcaa atcgccaagc gtggtgccgc tcgtcgggcg gccggactca ctcttgtagt
     4921 tctgcagcgc ctgctgctcc tcgtgaactt
//
LOCUS       DASSNN010000041         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_635353, whole genome shotgun sequence.
ACCESSION   DASSNN010000041 DASSNN010000000
VERSION     DASSNN010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635353"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFL84_00760"
     CDS             complement(<1..277)
                     /locus_tag="VFL84_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HET7607179.1"
                     /translation="MLFMVIERFKDNDMIPVYRRLREKGRMLPEGLKYIDSWIEPNFS
                     RCFQIIECDDLRRLQEWILQWRGSGTSFEIVPVLSSAETREVVAPYLD"
     gene            complement(294..914)
                     /locus_tag="VFL84_00765"
     CDS             complement(294..914)
                     /locus_tag="VFL84_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7607180.1"
                     /translation="MTAHLPILYGSRESGHAYKVKLALSLLGIAHEYREVDLSVPFAE
                     RRSDFRHASPYGEVPVWVDGSLRLAQSNAILIHLARETGRLGGDDIDLVTQWLFWEAN
                     RIGFSVPNLRHSLVFAKDTAPEVVAWLRSRAEQDLSRLDLQLADSAFILGAESSVVDV
                     ACCAYLFWPDQANLDLSAWPNVARWLDGIKSLRGWASPYELLKERH"
     gene            complement(911..1525)
                     /locus_tag="VFL84_00770"
     CDS             complement(911..1525)
                     /locus_tag="VFL84_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7607181.1"
                     /translation="MPPDITVRPATPDRWPDVEAIFGAKGCSVARGCWCMHYRRSGSA
                     ASLSKVSSRSRTYRAGLKALVDSGKPPGLVAYRDDVAVGWVSLAPREQFARLERSPVM
                     KPVDDTPVWSIICFVVPPEHRGQGVARALLKGAIAYAREQGATVIEAYPVDKPARSKA
                     DALWFGAKSMYDNAGFKEVARRKPARPVMRLVVSDRANARRSHR"
     gene            complement(1551..2309)
                     /locus_tag="VFL84_00775"
     CDS             complement(1551..2309)
                     /locus_tag="VFL84_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7607182.1"
                     /translation="MDLQLKNKTCLVTGASRGIGRGIARVLAAEGCRVAVVARRASLL
                     KELAEEIVAAGHARPLLLAEDLTADGVMQRIRDRVLAELGGLDVLVNNAGSSRPVPWN
                     ATEEQWQEGMRLNFELVRRLTNEFIPTMRAQRFGRIINITGANEPPNVNIASVAKAAL
                     HNWAKGLSRELAKDGITINCLPPGRINSEQILERLHPTAESRRAFIDANIPVGYFGEP
                     EDMAYLVAFLASPLARYITGEVIHVDGGMHRFAG"
     gene            2396..>2539
                     /locus_tag="VFL84_00780"
     CDS             2396..>2539
                     /locus_tag="VFL84_00780"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HET7607183.1"
                     /translation="MNHFFRRFAQATATAMGSHWAFGVAAIACLIWALTGPTFHYSNT
                     WQLV"
BASE COUNT          420 a          834 c          843 g          442 t
ORIGIN      
        1 tatccagata cggagcaacg acctcgcgcg tttcagcgct gctcagcacg ggcacgatct
       61 cgaacgacgt gccggagccg cgccactgaa ggatccattc ctgcagccgc ctcaagtcgt
      121 cgcactcgat gatctggaag cagcggctga agttcggctc gatccagctg tcgatgtact
      181 tgagtccttc gggcagcatg cggcctttct cgcgaagccg ccggtacacc gggatcatgt
      241 cgttgtcctt gaaacgctcg atcaccatga acagcatgtg ccacctcgtg cgttcagtgc
      301 cgctccttga gaagctcgta cggcgaggcc cagccgcgca ggcttttgat tccgtcgagc
      361 cagcgcgcga cgttcggcca ggcggagaga tcgagattgg cctggtccgg ccagaacaag
      421 taggcgcagc aggcgacgtc gacgacgcta gattctgcgc ccaagatgaa cgcgctgtcg
      481 gcgagctgaa ggtccaggcg cgagaggtcc tgctcggccc gagagcgtag ccacgcgacg
      541 acctcgggcg cggtgtcctt cgcgaagacg agcgagtgcc gcaggttggg gaccgagaag
      601 ccgatccgat tggcttccca aaagagccac tgcgtgacga ggtcgatgtc gtcgccgccg
      661 agtcggcccg tctcacgcgc cagatggatc aagatcgcgt tggactgtgc cagccgcaag
      721 ctgccgtcga cccacacggg cacttcgccg tacgggctcg cgtgccggaa gtcgcttcgc
      781 cgctcggcaa acggcacgct caggtcgact tctcgatact catgggcgat gccgagcaag
      841 ctcagcgcga gcttcacctt gtaggcatgg cccgactctc ggctgccgta caggatcgga
      901 agatgggcag tcatcgatgg ctccgtcttg cgttcgctcg atcgctcact acgagccgca
      961 tgacgggccg cgccggcttg cgtcgcgcga cttccttgaa gccggcgttg tcgtacatcg
     1021 actttgcgcc gaaccacagc gcgtcggcct tcgatcgcgc cggcttgtcg acgggataag
     1081 cctcgatgac cgtcgccccc tgctcgcgcg cgtaggcgat ggcgcctttg agcagcgcac
     1141 gggcgactcc ttgaccgcga tgctcgggcg gcacgacgaa gcagatgatc gaccagaccg
     1201 gcgtgtcgtc gacgggcttc atgaccggcg agcgctcgag cctcgcaaat tgctcgcgcg
     1261 gcgcgagcga gacccacccg acggcaacgt cgtcgcggta ggcgacgagg ccgggcggct
     1321 tgccgctgtc gacgagcgcc ttgaggccgg cacgataggt tctggagcgg ctcgaaactt
     1381 tcgacaagga agccgcgctg ccgctgcggc ggtagtgcat gcaccagcag ccgcgcgcga
     1441 cggagcaccc tttcgcgccg aagatcgcct cgacgtccgg ccaccggtct ggcgttgccg
     1501 ggcgcacggt gatgtccggg ggcataggcg agctcatcga ggccgaccgc tcaccccgcg
     1561 aagcggtgca tgccgccgtc gacgtggatg acctcgcccg tgatgtagcg agcgagcggc
     1621 gacgccagaa acgcgacgag gtacgccatg tcctcgggct cgccgaagta gcccacgggt
     1681 atgttggcgt cgatgaacgc gcggcggctc tcggccgtcg gatgcagccg ctccaggatc
     1741 tgctcgctgt tgatgcgtcc cggcggcaag cagttgatcg tgatgccgtc cttcgcgagc
     1801 tcgcgcgaga ggcccttcgc ccagttgtgc agcgcggcct tcgcgaccga tgcgatgttc
     1861 acgttcggcg gctcgttcgc ccccgtgatg ttgatgattc ggccgaagcg ctgcgcgcgc
     1921 atcgtcggaa tgaactcgtt cgtgagccgg cgcacgagct cgaagttgag ccgcatgcct
     1981 tcctgccact gctcttcggt cgcattccaa ggcacggggc gcgagctgcc ggcgttgttg
     2041 acgagcacgt cgaggccgcc gagctcggcg agcacgcggt cgcggatgcg ctgcatgacg
     2101 ccgtcggccg tcaaatcttc ggcgagcagc aacggccgag cgtggccggc ggccacgatc
     2161 tcttcggcga gctccttgag caggctcgcg cgccgtgcca cgacggcgac gcggcagcct
     2221 tccgcggcca gcacgcgcgc aatgccgcgg ccgatgccgc ggctcgcgcc cgttaccagg
     2281 caagtcttgt tcttcagttg cagatccatg gcgacggaat cttagtccag acgccgttcg
     2341 aaatggcatg cgagatgcag cgtcacgcgc accgaggcgg atcgcaggta tcactatgaa
     2401 tcatttcttt cgtcgatttg cgcaggccac cgcgacagcg atgggctcgc attgggcatt
     2461 cggcgtcgcg gccatcgcgt gcctgatctg ggcgctgacg ggccctacgt ttcactactc
     2521 gaacacgtgg cagctcgtc
//
LOCUS       DASSNN010000042         3523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_387553, whole genome shotgun sequence.
ACCESSION   DASSNN010000042 DASSNN010000000
VERSION     DASSNN010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387553"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VFL84_00785"
     CDS             complement(<1..427)
                     /locus_tag="VFL84_00785"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HET7607184.1"
                     /translation="MRFLLDFFYLIVCILLSPWLVYRLCTAGRRDFALRFGVGLRDSL
                     PSCIWLHGSSAGEVTVLRPLVALLERDHPGTPLVISAFTATGLAAARKLYARHRVVPL
                     PVDLSFVVARYLRSLNPRLLVVAESEFWPNLISSAHARGV"
     gene            complement(484..1824)
                     /locus_tag="VFL84_00790"
     CDS             complement(484..1824)
                     /locus_tag="VFL84_00790"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HET7607185.1"
                     /translation="MAEAQEENSSGKKQDARTRFIDQFLRQGQQGRLAGLVLGQLDAF
                     NRIATTFGHEQCDLFCADFADRLRETLPARTPILRLSERRFALLVDCDSMSAVMDIAM
                     ALTEQNPPQLTVGGDHFVVDITLGIAVYPSHADDAATLFRRAELALKEAREKDIAFDI
                     YRPDATQQQAALWKFETELQSAVSKGQLEVYYQPKVEIATQRVCGVEALVRWRTDSGN
                     FVPASDFIPLAETNGAIVPLTWLVFDKIIAQAASWTSMPEGFSVAVNVSPQIITHPEF
                     ATRVKALKAAVDAHKLKLTIELTEESLVQGDASSKASIERLRKLGIDLAIDDFGKGYS
                     SLTYLKQIPASEIKIDKRFIGTVAVDKTDKEIVKAIISLAHALGMRVVAEGVDSAEAV
                     ATVAELQCEMAQGFYIGRPMRGDLVPEWMAHYAAATPRRNTGVVRKLPEKARRQ"
     gene            2077..2355
                     /locus_tag="VFL84_00795"
     CDS             2077..2355
                     /locus_tag="VFL84_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607186.1"
                     /translation="MAKVKPSIRPTETYTEPNFLRVYTAEQPESNGQRLATIEHLSYT
                     AAGAGKRSGWSCTTIVDGEAMTKEDALFIARSYAAEHNIPVIYESHAE"
     gene            complement(2461..3186)
                     /locus_tag="VFL84_00800"
     CDS             complement(2461..3186)
                     /locus_tag="VFL84_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET7607187.1"
                     /translation="MLTKSFSRRALLLGLGLAAGAIALGDAGPAAAQQPLKIGIIGTG
                     NIGGALARHWVNAGHEVFVSSRHPEELEPLVKELGPRAHVGTPREAAAFGSVVLVSVP
                     YAAMPQIGADYASELAGKVIIDTSNPVERRDGAQAVEWQRKGAGLSTAELLKNNRVVR
                     AFNCIPAASLKGQANRQPARIAIPIGGDDAAAIAITERLVRDAGFDPVMVGTLAESRQ
                     FDLGQPLAQGNLSATELRSRMGR"
     gene            complement(3227..>3523)
                     /locus_tag="VFL84_00805"
     CDS             complement(3227..>3523)
                     /locus_tag="VFL84_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007818998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7607188.1"
                     /translation="LSVEQGYRAARLTALAMLGSIERELGDLDRVRAWTRVFGMVNSA
                     TGFNQQSRVMNGFSDLILELFGPDRGAHSRSSVGMAELPFDLPVEIEAELEIEP"
BASE COUNT          614 a         1155 c         1196 g          558 t
ORIGIN      
        1 gaacaccgcg cgcgtgcgca gaggagatca agttcggcca gaactcggat tcggcgacga
       61 cgagcagccg cggattcaac gagcgcaagt agcgcgcgac gacgaaagac aaatccacgg
      121 gcagcggcac gacgcggtgc cgcgcataga gcttgcgcgc cgccgcgagc cccgttgccg
      181 tgaatgccga aatgacgagc ggcgtgccgg gatggtcgcg ctcgagcagc gcgacgagcg
      241 ggcgcagcac ggtcacctcg ccggcggaag agccgtgcag ccagatgcac gacggcagcg
      301 agtcccggag gcccacgccg aaacgcagcg cgaagtcgcg tctccccgcc gtgcagaggc
      361 ggtacacgag ccacggcgag agcaggatgc agacgatgag gtagaaaaaa tcgagcagaa
      421 atctcatcgg caacttcgtg ggcgcctcga gcggcgcccg aagtttaccg catcgcagcg
      481 cacctattgg cggcgcgctt tctccggaag cttacgaacg acgccggtgt tgcggcgcgg
      541 cgtcgccgcg gcgtagtgcg ccatccactc cggcacgagg tcgccgcgca tcggccggcc
      601 gatgtagaag ccctgcgcca tctcgcattg gagctcggcg accgtcgcaa cggcttcggc
      661 gctgtcgacg ccttcggcga cgacgcgcat gccgagcgcg tgcgcgagcg agatgatcgc
      721 cttcacgatc tccttgtcgg tcttgtccac ggcgaccgtg ccgatgaagc gcttgtcgat
      781 cttgatctcg gaggccggga tctgcttcag atacgtcaac gacgagtaac ccttaccgaa
      841 atcgtcgatg gcgagatcga tcccgagctt tcgcaaccgc tcgatgctcg ctttggacga
      901 ggcgtcgcct tgcaccaagc tttcctcggt gagctcgatc gtgagcttga gcttgtgcgc
      961 gtcgacggcc gccttcaggg ctttgacgcg cgtcgcgaac tcggggtgcg tgatgatctg
     1021 cggcgagacg ttgaccgcca cggagaaccc ctcgggcatc gacgtccagc tcgcggcctg
     1081 cgcgatgatc ttgtcgaaca cgagccacgt cagcggaacg atggcgccgt tcgtctcggc
     1141 gagcggtatg aagtcgctcg ccggcacgaa gttgccggag tcggtccgcc agcgcacgag
     1201 agcttcgacg ccgcagacgc gctgcgtcgc gatctcgact ttgggctggt agtacacctc
     1261 gagctggccc ttcgaaacgg cgctctgcag ctcggtctcg aatttccaca gcgccgcctg
     1321 ctgctgcgtc gcatcgggcc ggtagatgtc gaacgcgatg tccttctcgc gcgcttcctt
     1381 gagcgcgagc tcggcgcggc gaaatagcgt cgccgcatcg tcggcgtgcg acgggtacac
     1441 cgcgattccg agagtgatgt cgacgacgaa gtgatcgccg ccgaccgtga gctgcggcgg
     1501 attctgctcg gtcagcgcca tcgcgatgtc catgaccgcc gacatcgagt cgcagtcgac
     1561 gagcagcgcg aagcggcgct ccgaaagcct caggatcggc gtgcgtgcgg gaagcgtctc
     1621 gcgcaagcga tccgcgaaat cggcgcagaa gaggtcgcac tgctcgtgac cgaacgtcgt
     1681 cgcgatgcga ttgaaggcgt cgagctggcc gagcaccagt cccgcgagcc tgccctgctg
     1741 cccctgccgc agaaactggt cgatgaatcg agtgcgcgcg tcctgctttt ttccggagct
     1801 gttttcttct tgagcctcgg ccacgggaaa tcccattagc gtcttgctag gaccggagcg
     1861 gcgatttgac cagaattcgc cgttcgtggc tgtgagagga atcacgacca cccgctttgg
     1921 ggtgccacga atttcgtgtc cacttgtgac gagccgcgtc agtcgcgcgt tgacataacg
     1981 gagcgggcgg ggccttggat cgccgctgcc gcgcgcctgg cacggccctt gcttttggct
     2041 tcggaagcca gcggatggcg agggtggaag cgagctatgg ccaaagtgaa gccgagcatc
     2101 aggcccaccg agacctacac ggagccgaac ttcctgcgcg tctatacggc cgagcaaccg
     2161 gagtcgaacg gccagaggct ggcgacgatc gagcacctgt cgtatacggc agccggcgcc
     2221 ggtaagcgct cgggctggag ctgcacgacg atcgtcgacg gcgaagccat gacgaaggaa
     2281 gacgcgctgt tcatcgcgcg cagctacgcg gccgagcaca acatcccggt catctacgaa
     2341 agccacgcgg agtaagcgcg cgagccggtc ggcaccgcgc gggcgagccg gttggatgcg
     2401 cgcgggcgag ccggttggtt acgcgcgggc gagccggctg gatccacgca ggcgagctgc
     2461 ctagcgaccc attcgcgagc ggagctcggt cgcgctgagg ttgccttgcg cgagcggctg
     2521 gccgagatcg aattgccgcg actctgcgag cgtcccgacc atgaccggat caaagcccgc
     2581 gtcgcgcacg aggcgctccg tgatcgcgat ggccgccgcg tcgtcgccgc cgatcggaat
     2641 cgcgatgcgc gccggttggc ggttcgcctg ccccttcaac gaagccgcgg gaatgcagtt
     2701 gaaagcgcgc acgacgcggt tgttcttgag gagctcggcc gtcgacaggc ccgccccttt
     2761 gcgctgccac tcgaccgcct gcgcgccgtc gcgccgctcg accggattgc tcgtgtcgat
     2821 gatgaccttg cccgcaagct cgctcgcgta atccgcgccg atctgcggca tcgccgcgta
     2881 gggcaccgag accagcacga ccgagccgaa tgcggcggcc tcgcgcggag tgccgacgtg
     2941 cgcgcgcggg ccgagctcct tcacgagcgg ctcgagctcc tcgggatgcc gcgacgacac
     3001 gaacacctcg tggccggcat tgacccaatg ccgtgccagc gcgccgccga tgttgcccgt
     3061 gccgatgatg ccgatcttca gcggctgctg cgcggcagcg ggccccgcgt cgccgagcgc
     3121 gatggcgccg gcagccagac ccaagccgag gagcagcgcg cgacgagaaa acgacttcgt
     3181 gagcatggcg ttccttcagt cgacgtgtgt cggtgacgat gatagctcag ggctcgatct
     3241 cgagctccgc ttcgatctcg accggcagat cgaacggcag ctccgccatg ccgacggagc
     3301 tgcgcgagtg cgcgccacgg tcggggccga acagctcgag gatcaagtcg gaaaagccgt
     3361 tcatgacgcg cgactgctga ttgaatcccg tcgccgaatt caccatgccg aagacgcgcg
     3421 tccaggcgcg cacgcgatcg aggtcgccga gctcgcgctc gatgctgccg agcatcgcga
     3481 gcgccgtgag ccgggccgcg cggtaaccct gctcgacgga caa
//
LOCUS       DASSNN010000043         2646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_887479, whole genome shotgun sequence.
ACCESSION   DASSNN010000043 DASSNN010000000
VERSION     DASSNN010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887479"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFL84_00810"
     CDS             complement(<1..647)
                     /locus_tag="VFL84_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7607189.1"
                     /translation="MTVRSVVGYAAVLAAAAGHSGFAAAQTVAVLSSNGVRAPIERLM
                     TQCTRAASREVSVTYGTSASIRQRVTGGEAVDAVLATSEVVGELAEAGQLDARSIAPL
                     GRAGIGVGIRTGTHRHEIGTAAEIKQALQDAKSVAYAADGASRPHIERMFERLGIAAE
                     MKAKSLLEQGSTRAAEKVVGGDAEILLTLVSEILPVEGLELLGPVPDEFQSYISF"
     gene            complement(644..1102)
                     /locus_tag="VFL84_00815"
     CDS             complement(644..1102)
                     /locus_tag="VFL84_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7607190.1"
                     /translation="MKSLARLFTAALGILTLCIPAFSNADVDPSVITFVAPKDIKFVR
                     NQAGTNETAVLYGDPSKPGPYVIQLRWLPGNMSRPHFHSTDRFFVVLEGTWWLGTGTK
                     FDPDSTVPVKAGTYVFHKAGGIHYDGAKDEPALIQVWGTGPMTTTQAEQK"
     gene            complement(1162..2406)
                     /locus_tag="VFL84_00820"
     CDS             complement(1162..2406)
                     /locus_tag="VFL84_00820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7607191.1"
                     /translation="MRDLKKALRGNRALLFGMALLTLGAGLQATLLGVRATLEGLSTF
                     ATGAVLASYYAGFVIGSIAAPTLVRRVGHIRVFAALTAMAAATILLQGIYFGPWAWAV
                     LRALSGVCFAGIYVVAESWLNDRADSTSRGVLLASYMVVVYVGLGAGQFLLNLADPSG
                     PTLFALIGILISLAVVPIALTAQRAPDFALPRRVSFRELLEISPLGAAGALFTGATAG
                     TLYTLGPVYAARSGFDTGAVATFMASAIMPAIALQLPLGRLSDRVDRRTVLMAISIGA
                     AVAAAIALQLGSASPSLFVAVATYGGLSLTAYAVVLAHVNDHLHPTQMVAASATVLLA
                     NGIGSALGPVAVAAAMQLAGPGAFFGSVIVLHATFTLYAAWRKHRTDAVPPPEKARFV
                     SASPQVAPTGRLAAPEAKPGET"
     gene            complement(2408..>2646)
                     /locus_tag="VFL84_00825"
     CDS             complement(2408..>2646)
                     /locus_tag="VFL84_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7607192.1"
                     /translation="LLVLADPEGAILPYDAIVLIARKRASDGVLRRALAPLIDAIPLA
                     LMQEANYRVDRDRDKETPAAAARWLEAEIAARGN"
BASE COUNT          467 a          896 c          888 g          395 t
ORIGIN      
        1 ctgaatgaaa tgtagctttg aaattcgtcg ggcacaggcc cgagcagctc gaggccttcg
       61 accggtagta tctcgctgac gagcgtcagc aaaatctcgg cgtccccgcc gacgactttc
      121 tcggccgcgc gcgtggaacc ctgctcgagc agcgacttcg ccttcatctc ggccgcgatg
      181 ccgagccgct cgaacatgcg ctcgatatgc gggcggctcg cgccgtccgc cgcgtaggcg
      241 acggacttcg cgtcctgcag ggcctgcttg atctcggcag ccgtgccgat ctcgtggcga
      301 tgcgtgccgg tgcgaatacc gaccccgatg ccggcgcggc cgagcggtgc gatcgatctc
      361 gcatcgagct ggcctgcctc cgcgagctcg ccgacgactt cgctggtcgc aagcactgcg
      421 tcgacggcct caccgcccgt cacgcgctgg cggatcgaag ccgacgtgcc gtacgtgacg
      481 gagacctcgc gggaagcggc acgcgtgcat tgcgtcatga gccgctcgat cggggcgcgc
      541 acgccgttcg acgacagcac ggcgaccgtt tgcgcagccg cgaacccgct gtgaccggcc
      601 gcggccgcga gcaccgccgc gtagcccacg acggaacgaa ccgtcacttc tgctcggcct
      661 gcgtcgtcgt catggggccc gtgccccaga cctggatcaa cgccggctcg tccttcgcgc
      721 cgtcgtaatg gatgccgccg gccttgtgaa acacgtacgt gccggccttc acgggcacgg
      781 tgctgtcggg atcgaacttc gttccggtgc cgagccacca cgtgccctcg agcacgacga
      841 agaaccggtc cgtcgaatgg aaatgcggcc ggctcatgtt gcccggcagc cagcgcagct
      901 gaatgacgta cggcccgggc ttcgacggat cgccgtagag aaccgccgtc tcgttcgtgc
      961 cggcttggtt gcgcacgaat ttgatgtcct tcggcgcgac gaaggtgatg acgctgggat
     1021 cgacgtcggc attcgagaac gcggggatgc aaagggtcag gattccgagg gctgcagtga
     1081 agagtcgagc gagacttttc atggcgcgtc cttcttttgc atgcgaagtg ggacttgcct
     1141 gatggtaggc cgaaacaaag ctcatgtctc gccgggcttg gcttcgggcg cggcgagccg
     1201 gcccgtcggc gcgacctgcg gcgacgcgct cacgaagcgc gccttttccg ggggaggcac
     1261 ggcgtcggtc ctgtgtttgc gccacgcggc gtacaacgtg aacgtcgcgt gcagcacgat
     1321 gacgctgccg aagaacgctc ccggccccgc gagctgcatt gcggccgcaa cggcgacggg
     1381 tccgagcgcc gacccaatgc cgttcgcgag cagcacggtg gcgctcgccg cgaccatttg
     1441 cgtcggatgc agatgatcgt tgacgtgcgc gaggaccacg gcataggccg tcagcgacaa
     1501 tccgccgtag gtcgcaaccg caacgaacaa cgaaggcgag gcgctgccga gctgcaaagc
     1561 gatcgcggcg gcaacggcag cgccgatcga tatcgccatg agaacggtgc gacggtcgac
     1621 gcgatccgac aaccggccga gcggcaactg caacgcaatg gccggcatga tcgcgctcgc
     1681 catgaatgtc gccaccgcgc ccgtatcgaa gccgctgcgc gccgcgtaca ccggaccgag
     1741 cgtgtacagc gtgccggccg tcgcgcccgt gaacagcgcg ccggcggcgc cgagcggcga
     1801 aatctcgagc agctcgcgaa agctgacgcg gcgcggcagc gcgaagtccg gcgcgcgttg
     1861 cgccgtcaac gctatcggga ccactgcaag cgagatcagg atgccgatca gcgcgaacag
     1921 cgtcgggccg ctcggatcgg cgagattcaa caagaactgg ccggcgccga gaccgacgta
     1981 gacgacgacc atgtacgacg cgagcagcac gccgcgcgag gtcgagtcgg cgcggtcgtt
     2041 gagccagctc tccgcgacga cgtaaattcc ggcaaagcac acgccggaaa gtgcgcgcag
     2101 gacggcccag gcccacgggc cgaagtagat tccctgcaac aggatcgtgg cggcggccat
     2161 cgccgtcagc gcggcgaaca cgcgaatgtg cccgacacgg cgcaccagag tgggcgcggc
     2221 gatcgagccg atcacgaagc ctgcgtaata cgaggccagg acggcgccgg tcgcgaacgt
     2281 cgacagtccc tcgagcgtgg cacgcacgcc gagtagcgtc gcctgcagcc cggccccgag
     2341 cgtcagcaac gccatgccga acagcaacgc tcggttgccg cgaagcgcct tcttcagatc
     2401 acgcatttca gttgccgcgc gccgcgatct cggcctcgag ccaccgtgcg gcggctgcgg
     2461 gcgtctcctt gtcccggtcg cgatcgacac gatagttcgc ctcttgcatg agagccagcg
     2521 gaatcgcatc gatcagcggt gcgagcgccc ggcgcaatac gccgtcgctc gccctcttcc
     2581 gcgcgatcag cacgatcgcg tcatacggca aaattgcgcc ctctgggtcg gcgagcacga
     2641 gcaagt
//
LOCUS       DASSNN010000044         3748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_136864, whole genome shotgun sequence.
ACCESSION   DASSNN010000044 DASSNN010000000
VERSION     DASSNN010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136864"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..522
                     /locus_tag="VFL84_00830"
     CDS             76..522
                     /locus_tag="VFL84_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607193.1"
                     /translation="MRNRNSGDCDAAAAAEAVTTQQQINVSLDVPHPEALECEATTVT
                     QYSQRNTVARVTGSVDIVNCPAGSTGAFTVVARVRDDSGEIKPLEFNETWQRNDATDV
                     NFTSDYPIGDNVELMNVRVRNLKCTCAAAADAAASEAPATANAPTN"
     gene            complement(519..1502)
                     /locus_tag="VFL84_00835"
     CDS             complement(519..1502)
                     /locus_tag="VFL84_00835"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HET7607194.1"
                     /translation="MSDAVPYERLTPDVIVGAVESYGFETTGSILGLASYENRVYQIG
                     VEQGFVVVKFYRPGRWTTDAILEEHEFALELAAQDIPVVPPIVHDGESLHVQDGYRFA
                     VYERRGGRWPELGTSEDREWLGRFLGRIHAVGATRRFTERPTLDRGEMGERAAEHVLE
                     GDWLPPHLVASYENVTEQLVDAVEQCFAEVGPYRELRIHGDCHRNNVLWTDDGPHFVD
                     LDDCMNGPAVQDLWLFLAGSRDEMAAQLGDILEGYGKFADFDYRELKLIEALRALRLI
                     HYTAWITRRWHDPAFPRAFPWLGESRYWEQHVQALLEQRATLDEPPLAIVV"
     gene            complement(1499..2401)
                     /locus_tag="VFL84_00840"
     CDS             complement(1499..2401)
                     /locus_tag="VFL84_00840"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET7607195.1"
                     /translation="MTKHVRTVAVVGTGDMGSAVGGALARAGYRVVTAGAGRSDLSRR
                     LADEAGIEDVGTLDAVVREAQLVLSIVPPAAATTFAAEAVGAMRVAGTRPAFADCNAV
                     APETVRAIARAIEATGAPFVDVGIVGRGPGVGRERTRFYVSGKARSAVLALGVPEIEL
                     IDLGEDVGTASAMKMAYAALNKGIDALLAAVLLAAERLGVRETLMTELQFSQAEALER
                     MQARVPFLGATAKRFAPEMAEIARTYESVGVTPDFHRGAEWVYSLFAATPFASETRAT
                     QPKRRSLDEALAALTAALTRERRA"
     gene            2460..>3748
                     /locus_tag="VFL84_00845"
     CDS             2460..>3748
                     /locus_tag="VFL84_00845"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HET7607196.1"
                     /translation="MRHSRRAVLSAGLGLAGTAVLRARSSRAAEITFADDPFTLGLAS
                     GYPEPTGVVLWTRLAPQPLVPGGGMPAAPVAVDWEVGSDEALRSVVRRGTVQATPDSA
                     HSVHVEVAGLTPARDYWYRFTSGGARSAVGRTRTAPASGTLPNRLTLAVACCQHFEQG
                     HYAAYRAIAADAPDLVVHVGDYIYEGSGTKQVRAHDAPECYTLDDYRQRYAIYKSDPH
                     LKAAHAAGPWMLTTDDHEVANNYAGEHSQLFTPPEVFLRRRAAAYKAYYEHQPLPRVM
                     APSEGRMRLYTSRAFGELAALHMLDGRQYRSPQACPPLPLVACDELYGRERTMLGGEQ
                     EQWLRGALAASSARWNLLGQQTVLAHFDQSGSDPRAYWADAWNGYPAARERLVATLLE
                     RPVANPVVFSGDIHAFLVNDVHAQALDNDSPIVAAELV"
BASE COUNT          585 a         1336 c         1284 g          543 t
ORIGIN      
        1 tcacctacgg ctaatcaggg cagcgccacc ggcaaaaacg tacagcagtg tcaagccgcg
       61 ccgggcggct cgcgcatgcg caaccgcaat tcgggggact gcgatgcggc cgctgccgca
      121 gaagctgtaa caacccagca acagatcaac gtctcgctag acgtgccgca tccggaagcg
      181 ctcgagtgcg aggccaccac ggtgacgcag tacagccagc gcaacaccgt cgcgcgcgtg
      241 acgggcagtg tcgacatcgt gaattgcccc gccggctcta cgggtgcgtt cactgtcgtc
      301 gcacgcgtca gggacgacag cggcgagata aagccgctcg agttcaacga gacatggcaa
      361 cgcaacgacg cgacagacgt gaatttcacg agcgactatc cgatcggcga caacgtcgag
      421 ctcatgaacg tgcgcgtgcg caacttgaag tgcacgtgcg cggccgcggc ggatgcggcg
      481 gcgtctgaag cgccggcgac cgcaaacgcg ccgacgaact agacgacgat cgcgagcggc
      541 ggctcgtcga gcgtggcgcg ctgctcgagc agcgcttgca cgtgttgctc ccagtagcgc
      601 gactcgccta gccacgggaa cgcgcgcggg aacgccggat cgtgccagcg gcgcgtgatc
      661 cacgccgtgt agtgaatgag ccgcaacgcg cgcagcgctt cgatcagctt gagctcgcgg
      721 taatcgaagt ccgcgaactt gccgtagcct tcgagaatgt cgccgagctg cgcggccatc
      781 tcgtcgcgcg agcccgcgag gaacaaccat aggtcctgca cggcggggcc gttcatgcag
      841 tcgtcgaggt cgacgaaatg cggtccgtcg tcggtccata gcacgttgtt gcggtgacag
      901 tcgccgtgga tgcgcagctc gcgatacggc ccgacctcgg cgaagcattg ctcgacggcg
      961 tcgacgagct gctccgtgac gttctcgtag ctcgcgacga gatgcggcgg cagccagtcg
     1021 ccctcgagca cgtgctcggc ggcgcgctcg cccatctccc cgcgatcgag cgtcggccgc
     1081 tccgtgaagc ggcgcgtcgc gccgacggcg tgaatgcggc cgaggaaccg gccgagccat
     1141 tcgcgatcct cgctcgtgcc gagctcgggc caacggccgc cgcgccgctc gtagacggcg
     1201 aagcggtacc cgtcttgcac gtgcaggctc tcgccgtcgt ggacgatcgg cggcacgacc
     1261 gggatgtctt gcgcggcgag ctcgagcgcg aactcgtgct cctcgaggat cgcgtccgtc
     1321 gtccagcggc cgggccggta gaacttcacg acgacgaagc cttgctcgac gccgatctga
     1381 tagacgcggt tctcgtagct cgcgagcccg aggatgctgc ccgtcgtctc gaagccgtag
     1441 ctctcgacgg cgccgacgat gacgtccggc gtcaggcgct catagggaac ggcgtcactc
     1501 acgcgcgccg ctcgcgcgtc agggcggccg tcagcgcggc gagcgcttcg tcgagcgagc
     1561 gccgcttcgg ctgcgtcgcg cgcgtctcgc tcgcgaacgg cgtcgcggcg aacaacgagt
     1621 agacccactc tgcgccgcga tgaaagtccg gcgtgacgcc gacgctttcg taggtgcgcg
     1681 cgatctcggc catctcgggc gcgaaccgct tcgccgtcgc gccgaggaac ggcacgcgcg
     1741 cttgcatgcg ctcgagcgcc tcggcttggc tgaactggag ctccgtcatc agcgtctcgc
     1801 gcacgccgag ccgctccgcc gcgagcaaca cggcggcgag cagcgcgtcg atgcctttgt
     1861 tcagcgcagc gtacgccatc ttcatcgcgg acgccgtgcc gacgtcttcg ccgaggtcga
     1921 tgagctcgat ctccgggacg ccgagcgcga gcaccgcgga gcgcgccttg cccgagacgt
     1981 agaagcgcgt gcgctcgcgg ccgacgccgg ggccgcgccc gacgatgccc acgtcgacga
     2041 acggcgcgcc cgtcgcctcg atcgcgcgcg cgatcgcgcg caccgtctcg ggcgcgacgg
     2101 cgttgcagtc ggcgaacgcg gggcgtgtgc cggcgacgcg catcgcgccg acggcctcgg
     2161 ccgcgaacgt cgtcgcggcg gcaggcggca cgatcgacaa cacgagctgc gcctcgcgca
     2221 cgaccgcgtc gagcgttccg acgtcctcga tgccggcttc gtccgcgagc cgccgcgaca
     2281 aatcgctgcg gccggcgccc gccgtcacga cgcgatatcc ggcacgcgcc agcgcgccgc
     2341 ccacggcgct gcccatgtca cccgtgccga ccactgccac cgttcgtaca tgcttcgtca
     2401 tcgcgcctac ccgtcaggtt gcgagcgtac aatcgcccga tcatagcggg aggtcgcgca
     2461 tgaggcattc gcggcgcgcg gttctgtcgg ccggtctcgg tctcgcggga acggcggtgc
     2521 tgcgcgcgcg ctcgtcgcgc gcggccgaga tcacgttcgc cgacgatccg ttcacgcttg
     2581 ggctggcctc gggctacccg gagccgaccg gcgtcgtgct ctggactcgg ctcgcgccgc
     2641 aaccgctcgt gccgggcggc ggcatgcccg cggcgcccgt cgccgtcgat tgggaggtcg
     2701 gctccgacga agcgctgcgc agcgtcgtgc ggcgcggtac cgtgcaggcg acgccggatt
     2761 cggcgcactc cgtgcacgtc gaggtcgccg gccttacgcc tgcgcgcgac tactggtatc
     2821 ggttcacgag cggcggcgcg cgcagcgcgg tcggccgcac gcgcacggcg cccgcgagcg
     2881 gcacgctgcc gaatcgcctg acgctcgccg tcgcatgctg tcagcacttc gagcaaggcc
     2941 actacgcggc ctatcgcgcg atcgccgcgg acgcgccgga tctggtcgtg cacgtcggcg
     3001 actatatcta cgaagggagt ggcacgaagc aggtgcgcgc gcacgacgcg ccggaatgct
     3061 acacgctcga cgactatcgg cagcgctacg cgatctacaa atccgatccg catctgaaag
     3121 ccgcacacgc agcgggaccg tggatgctta ctaccgacga ccacgaggtc gcgaacaact
     3181 acgccggcga gcattcgcaa ctgttcacgc cgcccgaggt gtttctgcgg cggcgcgccg
     3241 ccgcctacaa ggcgtactac gagcatcagc cgttgccgcg cgtgatggcg ccgtccgaag
     3301 gccgcatgcg cctctacacg agccgcgcat tcggagagct cgcggctctc cacatgctcg
     3361 acgggcgcca gtaccgctcg ccgcaggcct gcccgccgtt gccgctcgtc gcatgcgacg
     3421 agctctacgg acgcgagcgc acgatgctcg gcggcgagca ggagcaatgg ctgcgcggcg
     3481 cgctcgcggc gagcagcgcg cgctggaact tgctcggcca gcagacggtg ctcgcgcact
     3541 tcgaccagag cggcagcgac ccgcgcgcgt attgggcgga cgcctggaac ggctatcccg
     3601 ccgcgcgcga gcggctcgtc gcgacgttgc tcgagcggcc cgtcgcgaat cccgtggtgt
     3661 tcagcgggga catccacgcg ttcctcgtca acgacgtcca cgcgcaggct ttggacaacg
     3721 actccccgat cgtcgcagcc gagctcgt
//
LOCUS       DASSNN010000045         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1769989, whole genome shotgun sequence.
ACCESSION   DASSNN010000045 DASSNN010000000
VERSION     DASSNN010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769989"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2295)
                     /locus_tag="VFL84_00850"
     CDS             complement(<1..>2295)
                     /locus_tag="VFL84_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7607197.1"
                     /translation="DGHLVRLAEVAKVEIASREQNRIFRTNGALTTGFGIVKASTANT
                     VEVLDAVKAEVAQVNSELPEGMRLITSGDESLYIRAAIEEIYWTIGITTVLVGFVIFI
                     FLGSIRATLIPLVTIPVCLIATFSVLSLFDYSINLVTLLALALSIGLVVDDAIVVLEN
                     AHRRIEDGEAPLLAAFNGTRQVAFAVIATTVVLVSVFAPVAFLRDSIGRVFAELAVTI
                     AAAVIFSAVLALSLSPMMCSKLLRSSTKESKLTHTLDRAFVWLSNRYQSVLRSTLHAP
                     WLSVALCAAIAFGAYVLVQNIPQEYAPVEDRGQFNGQIQAPEGTSYDRLLASATKVEA
                     ALQKYFDDGSIQRGIVSVPGWGNNGSGMVNVTLKPWGERKIGTQDMLNDLNKQWEEIP
                     DIRVNAFMNSGNRGSGGGGGSPIQLVLGGPNYEELARWRDLIIARASENPGLVRLDSD
                     LRETQPQVLVRIDTDRAASLGVTARSIGSTLQTLMSERQVTTYVVDGEEYDVVLQAKP
                     EQRATYSDLSNIFVRADRTGELVPLSNLTKLEDMAGPSSLQRHNRMRAVTISGNLAPG
                     YTLGEALAYLENIIRTELPSTAQIDYRGESLEYKEASGALYFTFGTALFVVFLVLAAQ
                     FESFVHPLVIMVTVPLAVAGGLLGLWVSGKTLNIYSQIGLIMLVGIAAKNGVLIVEFI
                     NQLRDAGHEFTDAIIEAARIRFRPVIMTASATFMGSVPLILAEGPGSASRAALGVVIF
                     SGVTFATFFTLFIVPAVYNLMARRT"
BASE COUNT          421 a          731 c          771 g          372 t
ORIGIN      
        1 cgtgcggcgc gccatcaggt tgtagacggc cggcacgatg aacagcgtga agaacgtcgc
       61 gaacgtcacg ccggaaaaga tcacgacgcc gagcgccgcg cggctcgcgg agcccgggcc
      121 ttcggcgaga atcagcggca cggagcccat gaaggtggcc gacgcggtca tgatgacggg
      181 ccggaagcga atgcgcgccg cctcgatgat cgcgtccgtg aactcgtggc ccgcgtcgcg
      241 cagctgattg atgaactcga cgatcaagac gccgttcttg gcagcgatgc cgaccagcat
      301 gatcaggccg atctggctat agatgttcag cgtcttgccc gagacccaca gaccgagcag
      361 tcctccggcg accgcgagcg gcaccgtgac catgatgacg agcgggtgca cgaagctctc
      421 gaactgcgcc gcgagcacga ggaaaacgac gaagagcgcg gtgccgaacg tgaaatagag
      481 agcaccggaa gcttccttgt actcgagcga ctcgccgcgg tagtcgatct gcgccgtcga
      541 gggaagctcg gtgcgaatga tgttctcgag ataagcgagc gcctcgccga gcgtgtagcc
      601 gggcgccaga ttgcccgaga tcgtaacggc gcgcatgcgg ttgtgccgct gcagcgagct
      661 cggtccggcc atgtcctcga gtttcgtcag attcgagagc ggcacgagct cgcccgtgcg
      721 atcggcgcgc acgaagatgt tgctcaagtc cgaatacgtc gcgcgctgct cgggcttggc
      781 ctggagcacg acgtcgtatt cctcgccgtc gacgacgtag gtcgtgactt ggcgctcgct
      841 catcagcgtt tgcaaagtcg agccgatgct gcgcgccgtc acgccgaggc ttgcggcgcg
      901 gtccgtgtcg atgcgcacga gcacctgcgg ctgcgtctcg cgcaaatccg aatcgagccg
      961 cacgagcccc gggttctccg aggctctagc gatgatgagg tcgcgccagc gcgcgagctc
     1021 ctcgtagttc gggccgccga gcacgagctg gatcggcgat ccgccgccgc caccgctgcc
     1081 gcggttgccg ctgttcatga acgcgttgac gcgaatgtcg gggatctcct cccactgctt
     1141 gttcaggtcg ttcagcatgt cctgcgtgcc gatcttgcgc tcgccccacg gcttcagcgt
     1201 gacgttgacc atgccggacc cgttattgcc ccagcccggc acgctgacga tgccgcgctg
     1261 gatgctgccg tcgtcgaagt acttctgcag cgccgcctcg accttagtgg ccgatgcgag
     1321 caggcgatcg tagctcgtgc cctcgggcgc ctggatctgg ccgttgaact ggccgcgatc
     1381 ctcgacgggc gcgtattcct gcggaatgtt ttgcacgagc acgtaggcgc cgaacgcaat
     1441 cgccgcgcag agcgcgaccg acagccacgg ggcatgcagc gtcgagcgca gcaccgactg
     1501 gtagcggttc gatagccaga cgaacgctcg atcgagcgtg tgcgtgagct tgctctcctt
     1561 ggtgctcgag cgcagcagct tcgagcacat catcggcgac agcgacagcg cgagcaccgc
     1621 cgagaagatc acggcggccg cgatcgtcac ggcgagctcg gcgaagacgc ggccgatgct
     1681 gtcgcgcaag aacgcgacgg gcgcgaacac ggatacgagc acgacggtgg tcgcgatgac
     1741 ggcgaacgca acctggcgcg tgccgttgaa cgccgcgagc agcggcgcct cgccgtcctc
     1801 gatgcgtcga tgcgcgttct cgagcacgac gatcgcgtcg tcgacgacga gaccgatcga
     1861 gagcgcgagc gcgagcagcg tcacgaggtt gatcgagtag tcgaacaacg agagcaccga
     1921 gaacgtcgcg atcaggcaga ccgggatagt gacgagcgga atcagcgtcg cacggattga
     1981 gcccaagaag atgaagatca cgaagccgac gagcacggtc gtgatgccga tcgtccagta
     2041 gatctcctcg atcgcggccc ggatgtacag cgactcgtcg ccgctcgtga tgaggcgcat
     2101 gccctccggt agctcggaat tgacctgggc gacctcggct ttgacggcat cgagcacctc
     2161 gacggtgttc gcggtcgagg ccttgacgat gccgaagccc gtcgtcagcg cgccgttcgt
     2221 gcggaagatg cggttctgct cgcgcgacgc gatctcgacc ttcgcgacct cggccaagcg
     2281 aaccaagtga ccgtc
//
LOCUS       DASSNN010000046         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_388299, whole genome shotgun sequence.
ACCESSION   DASSNN010000046 DASSNN010000000
VERSION     DASSNN010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388299"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VFL84_00855"
     CDS             <1..1095
                     /locus_tag="VFL84_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7607198.1"
                     /translation="MLAQGVPNHLLFLDRDLRIVFANDVFLEAVGWSARKVVGLHISD
                     IVGMERFMERQQYYEKALAGETVEYDTTGAAGSEHGYFHFSYRPSFDDAGNVVGIFSM
                     ATDISERRKIQLELESKQAELLRSNKDLEQFAYVASHDLKAPLRAIELLVQWITEGLN
                     GYDVNNVQENLGLLAKRTQRLNRLLDDLLAYSRAGRKVGAHRMTDTHALVLDIVQMLN
                     PPETISISIDGQLPKFKTHPTPLETVMRNLISNAIKHHPGPQGRIVVSCQEEGDRYVY
                     SIEDDGEGIPKQYAERVFEMFQTLKPRDQVEGSGMGLAIVNRIVQWQGGRVWFDSPAP
                     SGKGTVFKFQWKKNQPVAANVETKACEAAG"
     gene            1077..1496
                     /locus_tag="VFL84_00860"
     CDS             1077..1496
                     /locus_tag="VFL84_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7607199.1"
                     /translation="MRSSRVVTILLVEDDEVDVKALKWAFDKLKVANPLVIARDGTEA
                     LEMLKDLPRPYLVITDINMPRMNGIELLRKIRATPELRDAIVFVLTTSNDEQDKIDAY
                     DLNVAGYMLKTDMGTSFSRAISLIDNYWKVVEFPDHE"
     gene            complement(1555..2805)
                     /locus_tag="VFL84_00865"
     CDS             complement(1555..2805)
                     /locus_tag="VFL84_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012809792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET7607200.1"
                     /translation="MQQNIVAHPELAAEGQRKIEWVRRNMPILRQLERQLGAERPFAG
                     RRIAVCIHLEAKTAYLALVLRAGGAAVAVTGSNPESTKDDVVAALAAEGLHVYARHGA
                     GPDEMRRYLGAALEIEPHVCIDDGGDLVELLHESHRAFGKEMLGACEETTSGVLRARA
                     RAKAGRLRFPVVLVNDARCKYLFDNVHGTGQSVWEGVMRSTNLVLAGKTVVIVGYGWC
                     GKGCALRAQGLGAHVVVCEVDPVKAADALMHGCRVLPLAEACRVADVLLTVTGVRHTL
                     RAEHFPLLKSNVLLANAGHFSEEIDVAALAKLAAERKELRANVEGFRLADGRWLNLLG
                     GGNIVNIACGDGHPAEIMDMSFALQALAARHVVERAGSLAADCHPVPVSVDEQVARLR
                     LAAAGVSIDTLTLDQQQYLAHWQA"
BASE COUNT          518 a          941 c          913 g          455 t
ORIGIN      
        1 atgctcgccc agggcgtgcc gaaccatctg ctgtttctgg acagggatct gcgcatcgtg
       61 tttgccaacg acgtctttct cgaggccgtc ggctggtcgg ctcgaaaggt cgtcgggctc
      121 catatctccg acatcgtcgg catggagcgc ttcatggagc gccagcagta ctacgagaaa
      181 gctctggccg gcgagaccgt cgagtacgac acgacgggcg cggccggcag cgagcacggc
      241 tacttccact tctcgtaccg gccgagcttc gacgacgccg ggaacgtcgt cggcattttc
      301 tcgatggcga ccgacatctc ggagcgccgc aagattcagc tcgagctcga gtcgaagcag
      361 gccgagctgc tgcgctcgaa caaggacctc gagcagttcg cgtacgtcgc gtcccacgac
      421 ttgaaagcgc cgttgcgcgc gatcgagctg ctcgtgcagt ggatcaccga aggtctgaac
      481 ggctacgacg tcaacaacgt tcaggaaaac ctcggccttc tggcgaagag gacgcagcgg
      541 ctgaaccggc tgctcgacga cttgctcgca tactcgcgcg ccggccgaaa agtcggcgct
      601 catcgcatga cggacacgca cgcgctcgtg ctcgacatcg tgcagatgct gaatcctccg
      661 gagacgatct cgatctcgat cgacggtcag ctgccgaagt tcaagacgca tccgacgccg
      721 ctcgagaccg tgatgcgcaa cctcatcagc aacgcgatca agcaccatcc cggcccgcag
      781 ggccgcatcg tcgtctcgtg ccaggaagag ggcgaccgct acgtctactc catcgaggac
      841 gatggcgaag gcataccgaa gcaatacgcg gagcgcgtat tcgagatgtt ccagacactg
      901 aagccgcgcg accaggtcga gggcagcggc atggggctcg cgatcgtgaa ccgcatcgtc
      961 cagtggcaag gcggccgcgt ctggttcgat tcccccgcgc cctcgggtaa agggaccgtt
     1021 ttcaaattcc agtggaagaa gaatcaaccg gtggccgcga acgtggagac aaaggcatgc
     1081 gaagcagcag ggtagtcacg atccttctcg tcgaggacga cgaggtcgac gtcaaagcgc
     1141 tcaaatgggc tttcgacaag ctcaaggtcg ccaatccact cgtgattgcg cgcgacggca
     1201 ccgaggcttt ggagatgttg aaggacttgc ctcggccgta tctcgtcatc acggacatca
     1261 acatgccgcg catgaacgga atcgagctgc tgcgcaagat cagggcgacg cccgagctgc
     1321 gcgacgcgat cgtgttcgtg ctgacgacgt cgaacgacga gcaggacaag atcgacgcct
     1381 acgatctcaa tgtcgcgggc tacatgctca agaccgacat gggaacgagc ttttcgcgag
     1441 cgatcagcct catcgacaac tactggaagg tcgtcgagtt ccccgaccac gagtgagcga
     1501 tcactcggcc cgagggcgct gacgcagcgc tcgcgcgcgt ccgcggcggc agcgtcacgc
     1561 ctgccagtgc gcgaggtact gctgctggtc caaagtcagc gtgtcgatcg agaccccggc
     1621 ggccgcgagc ctgagccgcg cgacctgctc gtctacgctc accggtaccg gatggcaatc
     1681 cgccgcaaga gagccggcgc gctcgacgac gtgccgcgcg gcgagcgcct gcagcgcgaa
     1741 gctcatgtcc atgatctcgg ccggatgccc gtcgccgcac gcgatgttga cgatgttgcc
     1801 gccgccgagc aggttcagcc agcgcccgtc ggcgaggcga aagccttcga cgttcgcgcg
     1861 cagctccttg cgctcggccg cgagcttcgc gagcgccgcg acgtcgatct cctccgagaa
     1921 gtgccccgcg ttcgcgagca gcacgttgct cttgagcagc gggaagtgct cggcgcgcag
     1981 cgtgtggcgc acgcccgtga cggtcagcaa gacgtcggcg acgcggcacg cttcggcgag
     2041 cggcaagact cggcagccgt gcatcaacgc gtcggccgct ttgacggggt cgacctcgca
     2101 gacgacgacg tgcgcgccga gcccttgcgc gcgcagcgcg cagcccttgc cgcaccagcc
     2161 gtagccgacg atgacgacgg tcttgccggc cagcacgaga ttcgtcgacc gcatgacgcc
     2221 ttcccaaacc gattgacccg tgccgtgcac gttgtcgaag agatacttgc agcgcgcgtc
     2281 gttgacgagc acgacgggga aacgcagccg gccggctttc gcgcgcgcac gcgcgcgcag
     2341 cacgccggac gtcgtctcct cgcacgcgcc gagcatctcc ttcccgaacg cgcgatgcga
     2401 ctcgtgcagc agctcgacga gatcgccccc gtcgtcgata cagacgtgcg gctcgatctc
     2461 gagcgccgca cccaagtagc ggcgcatctc gtcgggcccg gcgccgtggc gcgcgtagac
     2521 gtgcagccct tcggccgcga gcgccgcgac gacgtcgtcc ttcgtcgatt ccgggttgct
     2581 gcccgtgacc gcgacggccg ctcctccggc ccgcagcacg agcgcgaggt aggcggtctt
     2641 cgcttcgaga tggatgcaaa cggcgatgcg gcggcccgcg aacggcctct ctgcgccgag
     2701 ctgacgctcg agctgccgta gaatcggcat gttgcgccgc acccattcga tcttgcgctg
     2761 cccctcggcg gcgagctcgg gatgcgcgac gatgttctgc tgcatggcgg attccaagta
     2821 gagtcgc
//
LOCUS       DASSNN010000047         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1524038, whole genome shotgun sequence.
ACCESSION   DASSNN010000047 DASSNN010000000
VERSION     DASSNN010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524038"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VFL84_00870"
     CDS             complement(<1..746)
                     /locus_tag="VFL84_00870"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7607201.1"
                     /translation="MRFARFATARHANADDLCHRAEGSRDPLLHDAVPETEAMRYLAL
                     AVDYDGTAATHGQLAESARLAIERLRTSGRRVVLVTGRRVEDLLEVCPAIGSFDLVVA
                     ENGAVVYDPWRREDIVLGAAVPNSFVERLRERGVAPLEAGKVLVATTEPHGAAVFDAI
                     RELGLELQVIVNRSSIMVLPPGINKSTGLRFALHKLGLSQHEAVAIGDAENDHSFLRY
                     CECGVAVANAVASIKDAADFVTRGANGEGVA"
     gene            complement(836..1051)
                     /locus_tag="VFL84_00875"
     CDS             complement(836..1051)
                     /locus_tag="VFL84_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HET7607202.1"
                     /translation="MSKRLKVGDRVSWNSEAGRVRGTIVKVHTKDFAFKGYTHHATSD
                     DPQYEIKSDKTEHIAAHKGSALRKMRR"
     gene            complement(1075..2076)
                     /locus_tag="VFL84_00880"
     CDS             complement(1075..2076)
                     /locus_tag="VFL84_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HET7607203.1"
                     /translation="MSTKVAINGLGRIGRAILKLLIDEPRLELVAVNDLVDAENLAYL
                     LRFDTVYGRYSKPVAVIDGQLNVDGRKLHTLRERDPAALPWQELGVDVVFECTGALKK
                     REDLEKHIRAGAKFVLLSAPSQGAEVETVVHGANVLEGAPIIVSCASCTTNCITPLVE
                     IIGRRIGFKKAVMTTVHAYTSSQSIVDGASKNFRRGRAGAANLVPATTGAALATTRAL
                     PEHAGRFDGIAIRAPIPVGSIADLTFLTSRTTNAEEVNGILTAEAATARYAGVLGVSH
                     DPLVSADIVGDSRASIVDLELTKVVDGDLLKIMSWYDNEWGYANQMVRQAVSMTAAR"
     gene            complement(2073..>2359)
                     /locus_tag="VFL84_00885"
     CDS             complement(2073..>2359)
                     /locus_tag="VFL84_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HET7607204.1"
                     /translation="HTINTLPRKTSAAFSEHGKAAATLEQGLEEADDVLRDLAAAGID
                     LSQVTHRLLEEGIEKFVESFDDILQTIAAKSAGPAAGNASTTERARRPSA"
BASE COUNT          387 a          793 c          738 g          441 t
ORIGIN      
        1 gccacacctt cgccgttggc gccgcgcgtc acgaaatcgg cggcgtcttt gatcgaggca
       61 actgcgttcg ctaccgcgac tccacattcg cagtagcgaa ggaaagagtg gtcgttttcc
      121 gcatcgccga tcgcaaccgc ctcgtgctgc gatagaccga gcttgtgtag cgcgaatcgc
      181 agcccggtcg atttgtttat ccccggcggc aacaccatga tcgaggaccg attcacgatg
      241 acctgcagct cgagcccgag ctcccggatg gcgtcgaaca cggccgcgcc gtgcggctcc
      301 gtcgtggcga cgagcacctt gccggcctcg aggggagcga cgccgcgctc tcgcaggcgc
      361 tcgacgaagc tattcggcac cgctgcgccc agtacgatgt cctcacggcg ccaggggtcg
      421 taaacaaccg cgccgttctc ggccacgacg aggtcgaacg agccgatggc cgggcacacc
      481 tcgagcaggt cctcgacacg gcggcccgtg acaagcacta cgcgccgccc tgacgtgcgc
      541 agccgctcga tcgccagccg agcgctttcc gcgagctggc cgtgagttgc ggccgtgccg
      601 tcgtagtcga cggcgagagc caagtaacgc attgcctccg tctccggcac ggcgtcgtgg
      661 agcaacgggt cgcggctacc ttcggctcga tggcaaaggt cgtccgcatt cgcgtgccgg
      721 gccgtcgcga agcgtgcaaa cctcatgccg tatcgcatcc gagcgccgcg atcgccgacc
      781 cgcgacgaat tctatgcgaa gcgcccgcct gcacggcaaa cgccgctggc cgccctcacc
      841 ggcgcatttt tctcagcgcc gagcccttgt gggccgcgat gtgctcggtc ttgtcgctct
      901 tgatctcgta ttgagggtcg tcgctggtcg cgtggtgcgt ataacctttg aacgcgaagt
      961 ccttcgtgtg caccttgacg atcgtgccgc gcacacggcc cgcttcggaa ttccagctga
     1021 cgcgatcgcc gaccttgagt ctcttgctca tggcggacgt ttatcccgcc gtgctcagcg
     1081 agccgcggtc atggagacgg cctgacgtac catctgattc gcataacccc actcgttgtc
     1141 gtaccagctc atgatcttga gcaggtcgcc gtcgacgact ttcgtcagct ccaagtcgac
     1201 gatggacgcg cgcgaatcgc caacgatatc ggcggagacg agcggatcgt gcgacacgcc
     1261 aagcacaccg gcgtaccgcg ccgtcgcggc ttccgcggtg aggattccat tcacctcctc
     1321 ggcgttcgtg gtacgagacg tcagaaaggt caagtcggcg atcgagccga cggggatggg
     1381 cgctcgaatc gctatgccgt cgaaccggcc ggcatgctcg ggcagcgcgc gcgtcgtcgc
     1441 gagcgcggcg ccggtagtcg ccggcacgag gttggcggcc ccagcccggc cacggcggaa
     1501 gttcttgctg gcgccgtcta cgatcgactg cgacgacgta tacgcgtgca cggtcgtcat
     1561 cacggctttc ttgaacccga tgcgccggcc gatgatctcg acgagcggtg tgatgcagtt
     1621 cgtcgtgcag ctcgcgcacg agacgatgat aggcgcgccc tcgagaacgt ttgcgccatg
     1681 cacgacggtt tccacctccg cgccctgcga cggcgccgag aggagcacga atttggcgcc
     1741 ggcccggatg tgcttttcga gatcctcgcg cttcttgagc gcaccggtgc actcgaacac
     1801 aacgtcaacg ccgagctctt gccatggaag cgccgccgga tcgcgctcgc gaagcgtgtg
     1861 cagcttgcgc ccgtcgacat tgagctgacc gtctatcacg gcgaccggct tcgagtagcg
     1921 gccgtacacc gtgtcgaaac gaagcaagta ggcgaggttt tccgcgtcga ccagatcgtt
     1981 gaccgcaacc agctcgagcc gcggctcgtc gatcagcaac ttcagaatcg cacggccgat
     2041 gcgtcccaga ccattgatcg caactttggt gctcatgccg atggcctccg agccctttcg
     2101 gttgtgctgg cgttgcctgc cgccgggccg gcgcttttcg ccgcgatcgt ctgcaagatg
     2161 tcgtcgaatg actcgacgaa cttctcgatg ccttcctcga gtagccggtg cgtcacttga
     2221 gagagatcga ttcctgccgc tgccagatcc cgcagtacgt cgtctgcctc ctcgagcccc
     2281 tgctcgagcg tcgccgccgc cttgccatgc tcggaaaaag cggcgctggt ctttcgcggc
     2341 agcgtgttga tcgtgtgcg
//
LOCUS       DASSNN010000048         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1019062, whole genome shotgun sequence.
ACCESSION   DASSNN010000048 DASSNN010000000
VERSION     DASSNN010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019062"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VFL84_00890"
     CDS             <1..926
                     /locus_tag="VFL84_00890"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7607205.1"
                     /translation="TPEKYEQRTSGIHDLALHPKFAENSLVYFSYNKLGEVIPDSNPP
                     GRRQSAITVQRGKLEGMKLTNVQEIFTGEWSTGSSGSRLAFGTDGALYVSTGAPFGAT
                     TARDPASVYGKILRLRDDGKSAPGNPFATKAGARPEVFTMGHRDQLGLTVHPSGAVLA
                     AEHGPNGGDEVNLIQAGKDYGWPTWTYGRNYDGTRMSSLPVTEGIEQPLVLWVPSIAP
                     TGLTVYTGDKIPAWKNNLFVGSARRGEIPRTGGLERVVLNDKLEELRRERLLETLHQR
                     IRDVRQGPDGLLYVITDEDDGALLRIEPTAM"
     gene            949..1599
                     /locus_tag="VFL84_00895"
     CDS             949..1599
                     /locus_tag="VFL84_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607206.1"
                     /translation="MRARWQLGQWRGIPISLHWTVFIGIPWFLWQTRSLVDTGVAFVA
                     FGVLLLLHELGHAAVALSRRVEVHEIQLFFLHGSCAHELPDYELDDVLIAWGGVAAQF
                     VVLVVAFGADVLLAAFSPIAHVLASPLLRVFIATNIFIMIFNLLPIASFDGAKAWRIL
                     PLLADWAKETSLARKWRRRSAARETARTKKLEAESERIAADIIDKLKKGKSDAERS"
     gene            1655..>2281
                     /locus_tag="VFL84_00900"
     CDS             1655..>2281
                     /locus_tag="VFL84_00900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7607207.1"
                     /translation="MRALLAAVGTRGDVQPAIALALELRKRGHEARMCISPNFVEWAK
                     SLGLDARPMGVEMRAPSRGAGPPPKLTAEELRKLRESMPDLVTDEFVTIGAAVEGCDV
                     IVGANAHQYAGPSIAETRGIGCVTAVYAPVALPSPELAPPPTPGEPANARLGIDELWR
                     NTKKTWNERALERVNFNRGRLGLPPIDDVLEYTLTDHTWLAADAALALE"
BASE COUNT          387 a          757 c          768 g          369 t
ORIGIN      
        1 gcacacccga aaagtacgag cagcgcacga gcggcataca cgacctcgcg ctgcacccca
       61 agtttgccga gaactcgctc gtctacttct cgtacaacaa gctcggcgag gtcatccccg
      121 actcgaaccc gccgggccgc cgccagtcgg cgatcacggt gcagcgcggc aagctcgagg
      181 gcatgaagct aaccaacgtc caggagatct tcacgggcga gtggtcgacg ggctcgagcg
      241 gctcgcggct cgcgttcggc accgacgggg ccctctacgt gtcgaccggc gcgccgttcg
      301 gcgcgacgac ggcgcgcgat cccgcgagcg tctacggcaa gatcctgcgc ttgcgcgacg
      361 acggcaagtc ggcgccgggc aatccgttcg cgacgaaggc cggcgcgcgg cccgaggtct
      421 tcacgatggg ccaccgcgat cagctcgggc tcacggttca tccgagcggc gccgtgctcg
      481 cggccgagca cgggccgaac ggcggcgacg aggtcaatct gatccaggcc ggcaaagact
      541 acggctggcc gacgtggacg tacggccgca actacgacgg cacgcgcatg tcgtcgctgc
      601 ccgtcaccga gggcatcgag cagccgctcg tgctctgggt gccgtcgatc gcgccgacgg
      661 gcctgaccgt ctacaccggc gacaagatcc cggcgtggaa gaacaatctg ttcgtcggca
      721 gcgcgcgccg cggcgagatc ccgcgcacgg gcggtctcga gcgcgtcgtg ctcaacgaca
      781 agctcgagga gcttcgccgc gaacggctgc tcgagacgct gcatcagcgc attcgcgacg
      841 tccgccaagg gccggacggg ttgctctacg tgatcacgga cgaggacgac ggcgcgctgc
      901 tgcgcatcga gccaacggcg atgtaagggg agcttcggct cgccttagat gcgtgcacgg
      961 tggcagctag ggcagtggcg cgggattccg atctcgctcc actggacggt tttcatcggc
     1021 attccttggt tcctttggca aacgaggagc ctcgtcgaca cgggcgtggc gttcgttgcg
     1081 tttggcgttc ttcttctgct ccacgagcta ggtcatgccg cggttgcgct gtcgcgccgg
     1141 gtcgaagtgc acgagatcca attgttcttc ctgcacggca gttgcgcgca cgagctgccg
     1201 gattacgagc tcgacgacgt gctcatcgcg tggggcggtg ttgccgcgca attcgtggtg
     1261 ctcgtcgtcg cgtttggagc agacgtgttg ctcgcggcat tctcgccgat cgcgcacgtc
     1321 ctcgcttcgc cgctgcttcg agtgttcatc gcgacgaaca tcttcatcat gatcttcaat
     1381 ctgctgccca ttgcgtcgtt cgatggcgcc aaggcatggc gcattcttcc gctactcgcg
     1441 gactgggcca aagagacatc gttggcacgg aagtggcgtc ggcgatccgc tgcacgcgaa
     1501 acggcacgga caaagaagct cgaggccgag tccgagcgca tcgccgccga catcatcgac
     1561 aagctcaaga agggcaagtc ggatgctgaa cggagttaaa gtgaattcta ggacccctcc
     1621 accgagggcg atcggcgttc tctgagggcg tggcatgcgt gccctgttgg cagcagtagg
     1681 aacacgaggc gatgtgcagc ccgccatcgc gctcgcgctc gagcttcgca agcgcgggca
     1741 cgaggcgcgc atgtgcatct cgccgaactt cgtcgagtgg gcgaagagcc tcgggctcga
     1801 cgcgcggccg atgggcgtcg agatgcgcgc gccctcgcgg ggcgcgggtc cgccgccgaa
     1861 gctgacggcc gaagagttgc gcaagcttcg cgaatcgatg cccgacctcg tcacggatga
     1921 gttcgtgacg atcggcgcgg cagtcgaggg ctgcgacgtc atcgtcggcg ccaacgcgca
     1981 ccagtacgca ggtccgtcga ttgccgagac gcgcggcatc ggctgcgtga ccgcggtcta
     2041 tgcgccggtc gcactgccgt cgcctgagct cgcgccgccg ccgacgccgg gcgaacccgc
     2101 aaatgcgcgg ctcggcatcg acgagctctg gcgcaacacc aaaaaaacct ggaacgagcg
     2161 cgcgctcgag cgcgtcaact tcaaccgcgg ccggctcggc ctaccgccga tcgacgacgt
     2221 gctcgagtac acgctcacgg accacacgtg gctcgcggcc gacgccgcgc tcgcgctcga
     2281 g
//
LOCUS       DASSNN010000049         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1397518, whole genome shotgun sequence.
ACCESSION   DASSNN010000049 DASSNN010000000
VERSION     DASSNN010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397518"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..>2914)
                     /locus_tag="VFL84_00905"
     CDS             complement(95..>2914)
                     /locus_tag="VFL84_00905"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7607208.1"
                     /translation="LYLQVFYRDPKKDGTWRKPKSRTLSRAVVDGLPNQDDRYILTFL
                     AGATQIYSDYGYGGGYYGYETSLPARYRLVDPQTELLLPALCKTGRCRLRLRPEHDVN
                     DWLPVTWDEAEPWQFRLEVRRNERDERYEVRGALRRGNEWMDLAAPPLLVDAGIVFTR
                     DRAARLEHHGAFKWIALLRSQPSLSVPVSQADDFLVELFGRSAVPPLDLPEELRYEEV
                     ADTPRPRLAVKPMKESWQRDRLRGELSFDYGGTVITRGKPSRGVVQPAQRRVLLRDLG
                     AESAAEERLRELGWRAAPMAYSTAAEPPLELAANRLPRVVRELVAEGWHIEAEGKLYR
                     SPGRFELRVGSGIDWFELHGTVEFDGEVAQLPALLAALRRGETAVRLGDGSFGLLPEE
                     WLKRYGLFAELGSVQEDHVRFARNQIGVLDALLAAQPEIHCDELFAHARAKLERFGGV
                     HAAQPPAGFVGELRPYQREGVGWLGFLRDFGFGGCLADDMGLGKTVQILALLEARREL
                     RERSRSGNGPPSGAARALAAPSGPSLVVVPKSLIFNWRQEAARFAPRLRVLDYTGPLR
                     RRGRERFGDYDAVLTTYGTLRNDVADLKDVRFDYVILDEAQAIKNADSASAKAARLVQ
                     ADHRLALSGTPVENHIGELWSLVEFLNPGMLGAASVFRTARDAANALDDEMRTMLARA
                     LRPFILRRTKEQVIRDLPPKVEQTLYCELDGKQRKLYEELRDHYRGSLMKRIAREGIA
                     RAKIQVLEALLRLRQAAIHPGLLDERRAEDPSAKLEMLLHRLDEVVDEGHKALVFSQF
                     TAMLGIVRAELDRRGVRYEYLDGKTRNREARVARFQSDTDCKFFLISLKAGGLGLNLT
                     AAQYVFLLDPWWNPAVEAQAVDRAHRIGQTNQVFAYRLIARDTIEDKILELQNAKRDL
                     ADAIVGARDGLIRRLTKEDLELLLA"
BASE COUNT          492 a          989 c          937 g          496 t
ORIGIN      
        1 ggcacgcatg atttcatggc caacaacaag atccggatag cgtccgccgc gaacctcctg
       61 ctgggggcag ccatgtggtg aatgcatcac gcgctcacgc cagcagcagc tcgagatcct
      121 ctttggtcag gcgccggatc aagccgtcgc gcgcgccgac gatcgcatcg gccagatcgc
      181 gcttcgcgtt ctggagctcg aggatcttgt cttcgatcgt gtcgcgggcg atcagccgat
      241 acgcgaacac ctgattcgtc tgcccgattc gatgtgcgcg gtcgaccgct tgcgcctcga
      301 cggcgggatt ccaccagggg tccagcagaa agacgtactg cgcggccgtg aggttcaacc
      361 cgagaccgcc cgctttcaag ctgatcaaga agaacttgca atcagtgtcg ctctggaaac
      421 gcgcgacgcg cgcctcacgg ttccgcgtct tgccgtccag gtattcgtat cgaacgcccc
      481 ggcgatcgag ctccgctcgc acgattccaa gcatcgcggt gaactgcgag aaaaccaatg
      541 ccttgtggcc ctcgtcgacg acctcgtcga gccggtgcag cagcatctcg agcttggcgg
      601 aagggtcctc cgcgcgccgc tcgtcgagca atcccggatg aatcgccgct tgccgcagcc
      661 gcagtagcgc ttcgagaacc tggatcttcg ctcgcgcgat cccctctcgt gcgatgcgtt
      721 tcatgagact gccgcggtag tgatcgcgga gctcttcgta gagcttgcgc tgtttaccgt
      781 cgagctcaca gtagagcgtc tgctcgacct tcggcggcaa gtcgcgaatc acttgctcct
      841 tcgtgcgccg caaaatgaac gggcgaagcg cgcgtgcgag catcgtccgc atctcgtcgt
      901 cgagcgcgtt ggcagcatcg cgtgccgtcc tgaataccga cgcggcgccg agcattcccg
      961 gattcaggaa ctcgacgaga ctccagagct cgccgatatg gttctcgacc ggcgtgccac
     1021 tcagcgctag gcggtggtcc gcttgaacca gccgcgctgc ttttgccgaa gcggagtcgg
     1081 cgttcttgat cgcttgcgcc tcgtcgagga tcacatagtc gaagcgcacg tccttcaggt
     1141 cggcgacgtc gttgcggaga gtgccgtagg tcgtgagcac cgcgtcgtag tcgccgaaac
     1201 gctcgcggcc cctgcgccgc agcggtcccg tgtagtcgag cacgcgaagg cgcggagcga
     1261 atcgtgccgc ctcctgcctc cagttgaaga tcagcgactt cggcacgacc acgagcgaag
     1321 ggccgctcgg cgcagcgagc gctcgcgcgg cgccgctcgg cggcccgttg ccgctgcggc
     1381 ttcgctcgcg tagctcgcgc cgcgcttcga gaagcgcgag gatctgcacg gtcttgccga
     1441 gccccatgtc gtcggcgagg cagccgccga agccgaagtc gcgcaagaaa ccgagccagc
     1501 ccacgccttc ccgttgatat ggccgcagct cgccgacgaa accggcgggt ggctgcgcgg
     1561 cgtgtacgcc gccgaagcgt tcgagcttcg cccgagcgtg cgcgaacagc tcgtcgcaat
     1621 ggatctccgg ctgcgcggcg agcagtgcat cgagcacccc gatctggttg cgcgcgaagc
     1681 ggacgtggtc ttcttgtacg ctgccgagct cggcgaaaag accgtagcgc ttcagccatt
     1741 cttccggcaa caagccgaag gagccgtcgc cgagccggac cgctgtctcg ccgcgccgca
     1801 gcgccgcgag cagcgccggc agctgcgcga cctcgccgtc gaactcgacc gtcccgtgca
     1861 gctcgaacca gtcgataccg gagccgacgc gaagctcgaa tcgcccgggc gaccggtaga
     1921 gcttgccttc ggcttcgatg tgccagcctt ccgcgacgag ctcccggacc actcgaggaa
     1981 ggcggtttgc ggcgagctcg agcggcggtt ccgccgcagt cgaataggcc atgggggctg
     2041 cgcgccaacc gagctcccgc aagcgctcct cggccgcgga ttccgcgcca agatcgcgca
     2101 acagcacgcg gcgttgcgcc ggctggacca cgcctcgact cggttttccc cgcgtgatga
     2161 ccgtcccgcc atagtcgaag ctcaattcac cgcgcagccg gtcccgctgc cagctctcct
     2221 tcatgggctt gacagcgagc cgcgggcgcg gcgtgtccgc gacttcctcg tagcgcagct
     2281 cctcgggcaa atcgagcggc ggcacagcgg atcggccgaa cagctcgacg aggaaatcgt
     2341 cggcttgcga aacgggaacg gacagcgatg gttgcgatcg cagcaacgcg atccacttga
     2401 acgcgccgtg atgctcgagg cgcgcggcgc gatcgcgcgt gaacacgatg cccgcgtcga
     2461 cgagcaacgg cggtgccgcg agatccatcc attcgttgcc gcgccgcagc gctccgcgca
     2521 cctcgtagcg ctcgtcgcgc tcgttgcgcc gcacctcgag ccggaattgc catggctcgg
     2581 cttcgtccca cgtgacgggc agccaatcgt tcacgtcgtg ctcggggcgc agtcgcaacc
     2641 ggcatcggcc ggtcttgcac agcgccggca atagcagctc cgtctgcgga tcgacgagcc
     2701 ggtagcgagc cggaagcgag gtctcgtaac cgtagtaacc gccgccatac ccgtagtccg
     2761 aatagatctg agtcgcgccc gcgagaaacg tgaggatgta tcggtcatcc tggtttggga
     2821 gcccgtcgac cacggcgcga gagagcgtgc gcgatttcgg cttccgccag gtgccgtcct
     2881 ttttcggatc gcggtagaac acctgcaaat agag
//
LOCUS       DASSNN010000050         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_761983, whole genome shotgun sequence.
ACCESSION   DASSNN010000050 DASSNN010000000
VERSION     DASSNN010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761983"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..>2017)
                     /gene="ftsH"
                     /locus_tag="VFL84_00910"
     CDS             complement(33..>2017)
                     /gene="ftsH"
                     /locus_tag="VFL84_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET7607209.1"
                     /translation="RYIWWWFLGALLINFVITRFMVPPADAPLAIPYTVFKAQVESRN
                     VAEIYAQGATVEGAFVMPLTYPPEDEARSESADRPGRAERVEPRTSTTFTTTVPAFVD
                     PGLEALLVSHGVEISAVPIRNGASPLATLLLGFGPALLLIGFYVWIYRRAARGGIGGG
                     GLMGIGRSQAKRYDAESAAKVTFDDVAGIDEAENELVEIVDFLKAPERYTRLGGTAPK
                     GVLLVGAPGTGKTLLAKAVAGEAGVPFFSMSAAEFMEMIVGVGAARVRDLFKQARANA
                     PAIIFIDELDAIGRARGQFAIGGSNEQEQTLNQILTEMDGFSSRAGVIVLAATNQPDV
                     LDKALLRPGRFDRRVVVNLPDKTGRAEILKVHTRNVPLAGDVQLEEIAAATPGFSGAD
                     LKNVVNEAALLAARREQDVVRSKDFFDALEKIMLGPERPILLSRDDRERIAYHESGHA
                     VLGLVVPGADPVHRVTIVPRGQALGVTYQRPSTDRYNYPEAYLRARIVGMLGGRAAEE
                     LVYGTRTTGAESDIEQATSLARNMVTRWGMSEKLGLVQLAPRANPYLGGAPAFGADRP
                     FSEATAQTIDAEVLRIIRESYDEALKLLRMHRKVLDALVVALLEKETLDETEILATTG
                     LPRAAELPAAPGDALVRDATAGRAGREAAATPRARR"
BASE COUNT          290 a          714 c          687 g          326 t
ORIGIN      
        1 gcccgagctc gtgacggtgc cgctcctcga tctcaccgcc ttgctcgagg agtagcagcg
       61 gcctcgcgtc cggcgcgtcc ggccgtggcg tccctaacga gcgcgtcacc cggcgcagcc
      121 ggcagctcgg ccgcacgcgg caagcccgtg gtggcgagga tttccgtctc gtcgagcgtc
      181 tctttctcga gcagcgccac gacgagcgcg tccaagacct tgcggtgcat gcgcaacagc
      241 ttgagcgcct cgtcgtagct ttcgcggatg atccttagca cctcggcgtc gatcgtctgc
      301 gccgtcgcct cgctgaacgg ccggtcggcg ccgaacgccg gcgccccgcc cagatatgga
      361 ttcgcgcgcg gcgcgagctg gacgaggccg agcttctcgc tcatgcccca gcgagtcacc
      421 atgttccgcg caagcgaggt cgcctgctcg atatcgctct cggcgcccgt cgtgcgcgtc
      481 ccatagacca gctcttcggc cgcacggccg ccgagcatgc cgacgatgcg cgcgcgcagg
      541 tacgcttcgg gatagttgta gcggtcggtg ctcggccgct gatacgtcac gccgagcgcc
      601 tggccgcgcg gcacgatcgt cacgcggtgc acggggtcgg cgcccggcac gacgagcccg
      661 agcacggcgt gcccgctctc gtgataggcg atgcgctcgc ggtcgtcgcg gctcagcaaa
      721 atgggccgct ccggcccgag catgatcttc tcgagcgcgt cgaagaagtc cttcgacctg
      781 accacgtcct gctcgcgccg tgcggcgagc agcgccgcct cgttgacgac gttcttgaga
      841 tcggcccccg agaaccccgg cgttgcagcc gcaatctcct cgagctgaac gtcgccggcg
      901 agcggcacgt tgcgcgtgtg caccttcagg atttcggcgc ggcccgtctt gtcgggcagg
      961 ttcacgacga cgcggcggtc gaagcggccc gggcgcagca atgccttgtc gagcacgtcg
     1021 ggctgattcg tcgccgcgag cacgatgacg ccggcgcggc tcgagaagcc gtccatctcc
     1081 gtgaggatct ggttcagcgt ctgctcctgc tcgttcgagc cgccgatcgc gaactgaccc
     1141 ctcgcgcggc cgatcgcgtc gagctcgtcg atgaagatga tcgccggcgc gttcgcgcgg
     1201 gcttgcttga acaggtcgcg cacgcgcgcg gcgccgacgc cgacgatcat ctccatgaac
     1261 tcggcggcgc tcatcgagaa gaacggcacg cccgcttcgc ccgctacggc tttcgcgagc
     1321 agcgtcttgc cggtccccgg cgcgccgacg agcaagacgc ccttcggggc cgtgccgccg
     1381 agccgcgtat atcgctccgg cgccttgagg aagtcgacga tctcgacgag ctcgttctcg
     1441 gcttcgtcga ttcccgcgac gtcgtcgaac gtgaccttcg cggcgctttc ggcgtcgtag
     1501 cgcttggcct ggctgcggcc gatgcccatg aggccgccgc ccccgatgcc gccgcgcgcg
     1561 gcgcgccgat agatccagac gtagaagccg atcaggagca aagcaggccc gaaaccgagc
     1621 agcagcgtcg cgagcgggct tgcgccgttg cgaatcggca cggcgctgat ctcgacgcca
     1681 tgcgagacga gcagcgcctc gagccccggg tcgacgaacg ccggcacggt cgtcgtgaaa
     1741 gtcgtcgagg tccgcggctc gacgcgttcc gcgcggcccg ggcggtcggc gctctcggag
     1801 cgtgcctcgt cttcgggcgg gtacgtgagg ggcatgacga acgcgccttc gacggtcgcg
     1861 ccctgcgcgt agatttccgc gacgttgcga ctctcgacct gcgctttgaa gacggtgtag
     1921 ggaatcgcca gcggcgcgtc ggccggcggc accatgaagc gcgtgatcac gaagttgatc
     1981 agcagcgcgc cgaggaacca ccaccagata tagcgcg
//
LOCUS       DASSNN010000051         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_389213, whole genome shotgun sequence.
ACCESSION   DASSNN010000051 DASSNN010000000
VERSION     DASSNN010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389213"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..560
                     /locus_tag="VFL84_00915"
     CDS             138..560
                     /locus_tag="VFL84_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607210.1"
                     /translation="MSSIAGARITQVPVRHHARQFGQSKYGLSRIYKVLLDLISIRLV
                     VAYANRPLAWFFKMAIVPAALAAAFTAGGVVLALGGTYSLPLFGSALILALAVGFLLA
                     LGSLGELAKHLARRDVVPLVTASARYSKPVPFEPDVRI"
     gene            562..1551
                     /locus_tag="VFL84_00920"
     CDS             562..1551
                     /locus_tag="VFL84_00920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7607211.1"
                     /translation="MTDIDVIVPVGVRTDDLAALHRRRGEALRAAGYRASFVYVLDGE
                     IPQARETLAALARASDDARVIQLSRRFGETAAVLAGFASTKTEQLMILPAFEQVETAR
                     LGSVLDALAGADLVTVRRHPRCDSAVRRGQSYLFEAFLRRVGNTAFRDPGCTVHALRR
                     SVLEETQLYGEQHGFLPLLAANVGFKVVEIDLPQARRDAARRIERPRTYVHRLVDVLS
                     VFFLTRFTRRPLRFFGPVGAACTGLGSLGLAIVVVQRLILGVPLAERPALILSSLFVA
                     LGLQVLAIGLIGELIVFIHARSMRQYRVRDIIEAGSQAQRPKPALRSQAGGVD"
     gene            1556..>2014
                     /locus_tag="VFL84_00925"
     CDS             1556..>2014
                     /locus_tag="VFL84_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607212.1"
                     /translation="MVLVDGHVHVHECFDVGRLFDAAAANFAAAASALKGGRGSATAL
                     GAAAPRASARAHDAVLCLVESRGERFLAAVRAGRDRVWRGDHGYWEIELGPEPETCVA
                     RRGGTRLTLIAGRQIVTSERLEVLALGTTAPFPDGVAIEATLAAVRDAGAA"
BASE COUNT          263 a          701 c          696 g          354 t
ORIGIN      
        1 cgcgcgttgc taactggctt attggcaaga tcaccgggat tccgatccgg gacaacggct
       61 gctcattgaa ggcgtaccgt gcgtccgtca tgcggcacgt gcctttgtac tctgagatgc
      121 atcgcttcat cccggcgatg agctcgatcg caggcgcgcg catcacacag gtgcccgtcc
      181 ggcatcacgc ccggcagttc ggccagtcga aatacgggct ctcgcgtatc tacaaagtcc
      241 tgctcgactt gatctcgatc cgcctcgtcg tcgcgtacgc gaacaggccg ctcgcgtggt
      301 tcttcaagat ggctatcgtg ccggccgcgc tggccgcggc gttcacggcg ggcggcgtcg
      361 tgctcgcgct cggcggcacg tattcgctgc cgctgttcgg ctcggccttg attctcgcgc
      421 tcgcggtcgg cttcctgctc gcgctcggat cgctcggcga gctcgccaaa catctggcgc
      481 ggcgcgacgt cgtgccgctc gtgaccgctt cggcaaggta ttccaagccg gttccattcg
      541 agccggacgt gaggatctga catgacagac atcgacgtga tcgttcccgt gggagtcagg
      601 accgacgatc tcgccgcgct gcatcggcgc cgcggcgagg cgttgcgcgc ggccggctat
      661 cgcgcgtcgt tcgtctacgt cctcgacggc gagatcccgc aagcgcgcga gacgctcgcc
      721 gcgctcgcgc gcgcatcgga cgacgcgcgc gtgattcagc tgtcgcgccg gttcggcgag
      781 accgcggccg tgctcgcggg tttcgctagc acgaagacgg agcaactgat gatcctgccg
      841 gcgttcgagc aggtcgagac ggcgcgcctg ggaagcgtgc tcgatgcgct cgccggagcc
      901 gatctcgtga ccgtgcgccg tcacccgcgc tgcgactccg cggtcagacg cggccagagc
      961 tacctgttcg aggctttctt gaggcgggtc ggaaacacgg cgttccgcga cccgggctgc
     1021 accgttcacg ctctccgacg ctcggtgctc gaggagacgc agctatacgg cgagcagcac
     1081 ggcttcttgc cgttgctcgc cgcgaacgtc gggttcaagg tcgtcgagat cgacttgccc
     1141 caagcgcgcc gcgacgcggc acggcgcatc gagcgcccgc gcacctacgt gcaccggctc
     1201 gtcgacgtgc tgtccgtgtt cttcctgacg cgcttcactc gccggccgct gcgcttcttc
     1261 ggcccggtcg gagcggcgtg cacggggctc gggtcgctcg ggctcgccat cgtcgtcgtc
     1321 cagaggctga tcctcggcgt gccgctcgcc gagcggccgg cgctgatctt gagctcgctg
     1381 ttcgtggcgc tggggctgca agtgctcgcg atcgggctca tcggcgagct gatcgtcttc
     1441 atccatgcgc gctccatgcg ccagtatcgc gtacgcgaca tcatcgaggc cggctcgcag
     1501 gcgcagcggc cgaagcccgc gcttcgcagc caagcaggcg gcgtggactg atccgatggt
     1561 cctcgtcgac ggtcacgttc acgtgcacga atgcttcgac gtgggccgtc tgttcgacgc
     1621 cgcggcggcg aacttcgcgg ctgcggcgag cgcgctcaag ggggggcgcg gctcggcaac
     1681 cgctttaggc gccgccgcgc cgcgcgcatc cgcgcgcgcg cacgacgccg tgctctgcct
     1741 cgtcgagagc cgaggcgagc gattcctcgc ggccgttcgc gcggggcgcg accgcgtatg
     1801 gcgcggcgac cacggctact gggagatcga gcttggccct gagcccgaga cctgcgtcgc
     1861 gcgccgaggc ggcactcgtc tcacgctgat cgcagggcgc cagatcgtga cgagcgagcg
     1921 gctcgaggtc ctggcgctcg gcacgaccgc gccgttcccg gacggcgtcg ccatcgaggc
     1981 tactcttgcg gcggtgcgcg acgcaggcgc cgcg
//
LOCUS       DASSNN010000052         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1770486, whole genome shotgun sequence.
ACCESSION   DASSNN010000052 DASSNN010000000
VERSION     DASSNN010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770486"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2421)
                     /locus_tag="VFL84_00930"
     CDS             complement(<1..>2421)
                     /locus_tag="VFL84_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7607213.1"
                     /translation="IAQVDVQNRIQSVLPQLPQEVRDLGITVRKGSTDILLIVHMFAT
                     DSGVDRKYIANYANLQVRERLLRTPGVGDVNTNAARDYAMRVWIDPERAAARDLTVQE
                     IVAGLRNNNLQVAGGAVGAPPFDDNPAAYQLSIRAQGRLETPEEFADAIIKRDAQGRV
                     TRIGDVARVELGAQNYAINAYMNREPAVSLSVQQAPNTNALETWDNIKRTMEELERDF
                     PPGVAYDIIYNPVSFTRAAVDAVQETLVEAMLLAVIVVMVFLQRWRTAIIPLLAIPIS
                     LIGTLTIMSAFGFSLNNLSMFGLVLSIGIVVDDAIVVVENAERHLRAGMAPREAAHLT
                     MDEVGGALIGIALVLVAVFVPTAFLGGIAGQFYKQFALTIASATVISLVVSLTLSPAL
                     AAVILKPHGETRARTALGARLHALGERANSWLESVSGGYARLTGLLLRRGALLCVLYL
                     GLLVLTAWRVLDTPRGFIPAQDQGVVALTVTMPPGTSLARTDAVIQQVNPIVLGTPAV
                     SSTSVYAGMDGITFSPATNSGQLWAMFVPYEERLPQGLTANAIATEMRERLAPITAAE
                     IRIVNPASVRGMGNTGGFRMMIEDRGGLGYRALEDAVQQLAEAATADPAISLAFSNFN
                     VRNPTLDAVVDRDKAEMLGVPVRNVFATLQTYLAGVYVNNINLLGHTFQVIAQGDAAY
                     RQDEEWVGQLKTRSASGAMVPINAIATLKPATAPYRVLRYNLYPAADIQGDTAPGYSS
                     GEAIAAMERIAREVLPAGMYFEWTDLAYQQRISGDAGALAFALGVVFVFIFLAALYES
                     LTLPLAVI"
BASE COUNT          396 a          801 c          843 g          381 t
ORIGIN      
        1 aagatcacgg cgagcggcag cgtcaggctc tcgtagagtg ccgccaggaa gatgaacacg
       61 aacacgacgc cgagcgcgaa cgcgagcgcg ccggcgtcgc cagaaatgcg ctgctggtac
      121 gcgaggtcgg tccactcgaa gtacatgccg gccggcagca cttcgcgcgc gatgcgctcc
      181 atcgccgcga tcgcctcgcc cgacgagtag cccggcgccg tgtcgccctg gatgtccgcg
      241 gccgggtaga ggttgtagcg cagcacgcgg tacggcgcgg tggcgggctt cagcgtcgcg
      301 atcgcattga tcgggaccat cgcgccggaa gccgagcggg tcttgagctg cccgacccac
      361 tcctcgtcct gccgatacgc ggcgtcgcct tgtgcgatga cctggaacgt gtggccgagc
      421 agattgatgt tgttgacgta gacgccggcc aagtacgtct gcagcgtcgc aaagacgttc
      481 cgcaccggca cgccgagcat ctcggccttg tcgcggtcca cgacggcatc gagcgtgggg
      541 ttgcgcacgt tgaagttgct gaacgccagc gagatcgcgg gatccgcggt cgctgcctcg
      601 gcgagctgct gcacggcgtc ctcgagcgcg cggtagccta agccgccgcg gtcctcgatc
      661 atcattcgga agccgccggt gttgcccatg ccgcgcaccg atgcggggtt cacgatgcgg
      721 atctcggccg ccgtgatcgg ggcgagccgc tcgcgcatct cggtagcaat cgcgttcgcc
      781 gtgagccctt gcggcaggcg ctcctcgtac ggcacgaaca tcgcccagag ctgacccgag
      841 ttcgtcgccg ggctgaacgt gatgccgtcc atgccggcgt agacggacgt cgacgagacg
      901 gccggcgtgc cgagcacgat cggattgacc tgctggatga cggcatccgt gcgcgcgagg
      961 ctcgtgccgg gcggcatcgt gaccgtcagc gcgacgacgc cctggtcttg cgccggaatg
     1021 aagccgcgcg gcgtgtcgag cacgcgccaa gcggtcagca cgagcagccc caagtacagc
     1081 acgcacaaca gcgcgccgcg ccgcagcagc aggcccgtga gccgcgcgta gccgccgctt
     1141 acgctctcga gccacgagtt ggcgcgctcg ccgagcgcgt gcagccgtgc gccgagggcg
     1201 gtccgcgcgc gcgtctcgcc gtgcggcttc aggatcacgg ccgcgagcgc gggcgacaag
     1261 gtcagcgaca cgacgagcga gatcaccgtc gccgacgcga tcgtcaacgc gaactgcttg
     1321 tagaactggc ccgcgatgcc gcccaagaat gccgtcggca cgaatacggc gacgagcacg
     1381 agcgcgatgc cgatcagcgc gccgccgacc tcgtccatcg tgaggtgcgc cgcctcgcgc
     1441 ggcgccatgc cggcacgcaa gtggcgctcg gcgttctcga cgacgacgat cgcatcgtcg
     1501 acgacgatgc cgatcgagag cacgaggccg aacatcgaca ggttgttcag cgagaagccg
     1561 aacgccgaca tgatcgtcag cgtaccgatc agcgagatcg gaatcgcgag cagcgggatg
     1621 atcgccgtgc gccagcgctg caggaacacc atgacgacga tgacggcgag gagcatcgcc
     1681 tcgacgagcg tttcctgcac cgcgtccacg gcagcgcgcg tgaacgacac ggggttgtag
     1741 atgatgtcgt aggcgacgcc gggaggaaaa tcgcgctcga gctcctccat cgtcctcttg
     1801 atgttgtccc aggtctcgag cgcgttcgtg ttcggcgcct gctgcacgga aagagagacg
     1861 gcgggctcgc ggttcatgta cgcattgatc gcgtagttct gcgcgccgag ctcgacgcgc
     1921 gccacgtcgc cgatgcgcgt cacgcggcct tgcgcgtcgc gcttgatgat cgcgtcggcg
     1981 aactcttcgg gcgtctcgag ccggccttgc gcgcgaatgc tgagctgata agcggcggga
     2041 ttgtcgtcga acggcggcgc gccgacggcg ccgccggcga cttggagatt gttgttgcga
     2101 agccccgcga cgatctcctg gaccgtgaga tcgcgggcgg ccgcgcgctc gggatcgatc
     2161 cagacgcgca tcgcgtaatc gcgcgccgcg ttcgtgttca cgtcgccgac gcccggcgtg
     2221 cgcaggaggc gctcgcgtac ctgcaggttc gcgtagttcg cgatgtactt tcggtcgacg
     2281 ccgctgtcgg tcgcgaacat gtgcacgatc agcaggatgt ccgtcgagcc cttccgaacc
     2341 gtgatgccga ggtcgcggac ttcctgcggc agctgcggca gcacggattg gatgcggttt
     2401 tgcacatcca cctgtgcgat g
//
LOCUS       DASSNN010000053         4912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_888492, whole genome shotgun sequence.
ACCESSION   DASSNN010000053 DASSNN010000000
VERSION     DASSNN010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888492"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1089
                     /locus_tag="VFL84_00935"
     CDS             163..1089
                     /locus_tag="VFL84_00935"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HET7607214.1"
                     /translation="MRRSLAALIGVAAGACLAACAGRGDEQAGATAPDAPAAAARPDH
                     KNIAYAIGGQTVRLVDGVAEVQAAPGSAAKVVARYFGNEARGDLNADGRADVAFLLTL
                     EPGGSGTFFYAVAALDLPGGLVGSQGLLIGDRIAPQSTELRPDGVIAVNYADREPDES
                     FASAPSVAKTILLKLNPSTMRLGEVAQDFEGEADPASMKLDMKTWTWVRAVQDGNEIV
                     PRNAQAFTLTFGTDGMFAATTDCNRVRGGYTARGRDLSFDDALVATRMFCADSQEQVF
                     TALLQNTSGYTFTSRGELVLQLTDGAGSATFR"
     gene            complement(1086..1964)
                     /locus_tag="VFL84_00940"
     CDS             complement(1086..1964)
                     /locus_tag="VFL84_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006381822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET7607215.1"
                     /translation="MKVVITGGSGQLARRVAELLLVKIAPQDLIVTTRTPARLRSLAE
                     RGVVVRQADFAAPQTLRGAFAGAGRMLLVSATDLGLRTAQHRAAIDAAKAAGVRHVVY
                     TSGLRPEPPNPAVVAPSHYATEQALMQSGLAWTILRNSLYADYQIPEGSQAIAAGALV
                     HNRGAGKVAYVARDDCAAVAAAVLVAAGQAGKCYDVTGPKAYAAAELAALYAELGGQP
                     VVERCVSDAELVAGIVGKAEGDDHLRYGAELVASFGRSIREGYMDARTAHVEKLTGRA
                     PKSLRQVLEPLRAVRR"
     gene            complement(2016..>4912)
                     /locus_tag="VFL84_00945"
     CDS             complement(2016..>4912)
                     /locus_tag="VFL84_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7607216.1"
                     /translation="LLDGRRAMPINAGMSVSINTIPAAAIARVETITGGASSVYGADA
                     VAGVVNFITKRDFEGVDVDTQYGQTAEGDGMEFRYSVVLGTNAFDNKGNVMLGFERSK
                     RDEIFAVERDFRLAGYADPSALSQTDFYSAAGYSPVATNMHNRNVLLGIFGVPNTPAF
                     ATAGQYYMNSDGTLYKETADGAFRYNGPFVDEQGATWRKREVSNASTNGQLRENELAE
                     YLQIPLDRYSLFARGHYDLTDNLRAFADVLYSRSQTEQRQAASPMLGGWRASAPHGNG
                     VYAPSVVSLGADGLPGTGDTGENMATRAEYLPGGRFGLACGPAGGCTKSQAFPTPPEL
                     TLLLDSRPNPEADWEFRQTTLFADPRRSYVEVGSYQLIGGLEGDLEFKDWTWELYASS
                     GSTYTESTYGGGVSLERYRFVMRQPNYGRGLLYIGNELGAGFASGSISCTSGLPAVYG
                     VSGYDEDFVPSQDCQAAIRARSKASGEMRQNIVEYNLQGAIAELPAGEMRFALGLADR
                     ENRFEFKPDPLNSQESVLDQLAGFFPVGDAEGKTEATELYGELLLPIVSDVWGARDLS
                     LELGYRATDNEPSNDVDTWKALLDWRVVERVRVRGGRQVANRAPNIGELFQSQEQFAP
                     FSLFQGDPCSELNPATLTYTANPTLNPAGPATAAQVKGLCQQLMGPTGAAEYYGGGTP
                     QPSGFVSPRISNLSGNPDLRSEEARTLTLGVVANLTDRATFTLDYWRIELDDMIAPQV
                     PDTLYRECLNFDTNPTYSPTHPSCELIVRNPINGAVATVDVTYTNEAAADLAGYDLQF
                     DWSTNDAGPGALSLNVLATLTDHWKTRVNPTSPWLDFTGTTGPSDVRGVNQFAYDYRL
                     FTTVGYRTSKWGTTLRWRYLPSILPEGTIRTQARYHETPSYGVFDVAGRYMFADKWEL
                     RFGVDNVLDEEPPIVNLRTVAEGYTQTGVTNASFYDVLGRRYYVGMTVEF"
BASE COUNT          804 a         1651 c         1644 g          813 t
ORIGIN      
        1 tcgtgtggcg tggcacggtg tccgacacgt tgcccgacaa cgtgcagaag gccgagaagc
       61 tcgtggtcag ggcgatcaac aagatggcgg agcaaggacg caagctctgg gaaaaggagc
      121 tggaccgccg agaggacacg gcacgcggca aggactgagc ccgtgcgccg aagcctcgcg
      181 gcgttgatcg gcgttgccgc cggcgcgtgc ctcgctgcct gcgccggccg cggcgacgag
      241 caagcgggcg cgacggcgcc ggacgcgcct gcggcggccg cgcggccgga tcacaagaac
      301 atcgcctacg cgatcggtgg ccaaaccgtt cggctcgtcg acggcgtcgc cgaggtgcag
      361 gctgcgccgg gctccgccgc caaagtcgtc gcgcgctact ttggcaacga agcacgcggc
      421 gacctgaacg ccgacgggcg cgccgacgtc gcattcctgc tgacgctgga gccgggaggc
      481 agcggcacgt ttttctacgc ggtagcggcg ctcgatctgc cgggcggcct cgtcggatcg
      541 caggggctct tgatcggcga tcgcatcgca ccgcagtcga cggagctgcg gcccgacggc
      601 gtcatcgccg tcaactacgc cgaccgcgag cccgacgaga gcttcgcgag cgcgccgtcg
      661 gtcgccaaga cgatcctgct gaagctcaat ccaagcacga tgcggctcgg cgaagtcgcg
      721 caagatttcg agggcgaagc cgatcccgcc tccatgaagc tcgacatgaa aacgtggacg
      781 tgggtacgcg cagtgcaaga cggcaacgag atcgtgccgc gcaacgcgca agcgttcacg
      841 ctgacgttcg gcacggacgg catgttcgcg gcgacgacgg attgcaaccg tgtccgaggc
      901 ggctacaccg cgcgaggacg cgacctcagt ttcgacgacg cactggtcgc gacgaggatg
      961 ttctgcgcgg actcgcaaga gcaggtcttc acggccctgc tgcaaaacac gtcgggctac
     1021 acgttcacgt cgcgcggtga gctcgtcctt cagcttacgg acggcgcagg ctccgcgacg
     1081 tttcgctagc ggcggaccgc acgcagcggc tcgagcactt gccgcagcga cttgggcgcc
     1141 cttcccgtga gcttttccac gtgcgccgtg cgtgcgtcca tgtagccctc gcggatcgag
     1201 cggccgaacg acgcgacgag ctcggcgccg tagcgcagat ggtcgtcgcc ttccgccttg
     1261 ccgacgatcc cggccacgag ctcggcgtcg ctgacgcagc gctcgacgac gggctgcccg
     1321 ccgagctcgg catacagcgc cgccagctcg gccgcagcgt aggccttcgg ccccgtcacg
     1381 tcgtagcact tgccggcttg accggccgcg acgagcacgg cggccgcaac ggcggcgcag
     1441 tcgtcgcgcg cgacgtacgc gaccttgcca gcgccgcggt tgtggacgag agcgccggcg
     1501 gcgatcgctt gcgacccctc cggtatctgg tagtcggcgt acaagctgtt gcgcaggatc
     1561 gtccacgcca ggccgctctg catgagcgcc tgctcggtcg cgtagtggct cggcgcgacg
     1621 acggccggat tcggcggctc cggtctgagg cccgaggtgt agacgacgtg gcgcacgccg
     1681 gcggccttcg ctgcatcgat cgcggctcga tgctgtgcgg tgcggaggcc tagatccgtg
     1741 gcgctgacga gcagcatccg cccggcaccc gcgaaagcgc cgcgcagcgt ctgcggcgcc
     1801 gcgaagtcgg cctgacgaac cacgacgccg cgctcggcga gcgagcgcag cctggccggc
     1861 gtgcgcgtcg tgacgatcag gtcctgcggc gcgatcttca cgagcaatag ctcggcaacc
     1921 cgtctcgcga gctgccccga gcctcctgtg atcacgactt tcattgcatg aaccggcccc
     1981 ggcccttcac cggggccggc tctcgcgcag cggcatcaaa actcgaccgt catccccaca
     2041 tagtagcggc gcccaagcac gtcgtagaac gacgcgttgg tcacgccggt ttgggtgtag
     2101 ccttcggcca ccgtacgcag attgacgatc ggcggctcct catcgagcac attgtcgacg
     2161 ccgaagcgca gttcccattt gtccgcgaac atgtaccgtc ccgcgacgtc gaagacgccg
     2221 tagctcggcg tctcgtgata tcgcgcctgc gtgcggatcg tgccttcggg caggatgctc
     2281 ggcagatacc gccagcgcag cgtcgtgccc cacttggagg ttcggtaacc gaccgtcgtg
     2341 aacaggcggt agtcgtaggc gaactggttc acgccgcgca cgtcgctcgg cccggttgtg
     2401 cccgtgaaat cgagccacgg cgaggtaggg ttcacgcgcg tcttccaatg gtcggtgagc
     2461 gtagcaagca cgttgaggct gagtgcgccc gggccggcgt cgttagtact ccagtcgaac
     2521 tgcaggtcgt agcccgcgag atccgccgcc gcttcgttcg tgtacgtgac gtcgaccgtc
     2581 gccactgcgc cgttgatagg attgcgcacg atcagctcgc atgacggatg cgtgggactg
     2641 taggtcgggt tcgtgtcgaa gttcagacac tcacggtaca acgtgtccgg cacctgcggc
     2701 gcgatcatgt cgtcgagctc gatgcgccag tagtcgagag taaacgtagc tctgtcggtg
     2761 agattcgcga cgacgccgag cgtcagcgtt cgggcctcct cggagcgcag gtccggattg
     2821 ccgctcaagt tcgagatgcg cggcgagacg aacccgctcg gctgcggtgt accgccgccg
     2881 tagtactccg ccgcgcccgt cggacccatc aattgctggc aaagcccctt cacctgcgcg
     2941 gccgtcgcgg gccccgcggg gttcaaggtc ggatttgccg tgtacgtcaa ggttgcggga
     3001 ttgagctccg agcacggatc gccctgaaag agactgaacg gcgcgaactg ctcctgcgac
     3061 tggaacagct cgccgatgtt cggcgcgcgg ttcgcgacct gccggccgcc gcgcacgcgg
     3121 acgcgctcga cgacccgcca gtcgagcagc gccttccacg tgtcgacatc gttgctcggc
     3181 tcgttatcgg tcgctcgata gccgagctcg agactgagat cccgtgcgcc ccaaacgtcg
     3241 ctcacgatag gcaacagcag ctcgccgtac aactccgtcg cctcggtttt tccctccgca
     3301 tcgccgaccg gaaagaaacc cgcgagctgg tcgagcacgg actcttgcga gttcagcgga
     3361 tccggcttga actcgaagcg gttctcgcgg tcggcgaggc ccagagcgaa acgcatctcg
     3421 ccggccggaa gctccgcgat agcgccctgc aggttgtact cgacgatgtt ctggcgcatc
     3481 tccccggacg ccttcgaccg cgcgcgaatg gcggcctgac agtcttgcga cggcacgaaa
     3541 tcctcgtcgt acccgctgac gccgtagacg gcaggcagcc cgctcgtgca gctgatcgag
     3601 cccgaggcga atcctgcgcc gagctcgttg ccgatgtaga gcaggccccg gccgtaattc
     3661 ggttgccgca tgacgaagcg gtagcgctcg agcgagacac cgccgccgta tgtgctctcc
     3721 gtgtacgtcg aacccgacga cgcgtacagc tcccaggtcc agtccttgaa ttccagatcg
     3781 ccttccaacc cgccgatgag ctgataggaa ccgacctcga catagctgcg gcgtggatcg
     3841 gcaaacagcg tcgtttggcg gaattcccag tcggcctcgg gattcggccg actatcgagc
     3901 aacagcgtga gctccggcgg cgtcggaaag gcctgcgact tcgtgcaacc gcccgcgggt
     3961 ccgcaggcga ggccgaagcg gccgcccggc agatactcgg cgcgcgtcgc catgttctcg
     4021 ccggtgtcgc cggtgccggg caaaccgtcc gcgccgagag aaacgaccga cggcgcgtag
     4081 acgccgttgc cgtgcggcgc gctcgcgcgc cacccgccga gcatcggcga ggcggcctgc
     4141 cgctgctccg tctgtgaccg gctgtataga acgtcggcga acgcgcgcag gttgtcggtc
     4201 agatcatagt ggccgcgcgc gaacaacgaa tagcgatcga gcgggatctg caggtattcc
     4261 gccagctcgt tctcgcgcag ctgaccgttc gtcgacgcgt tcgatacctc tcgcttcctc
     4321 catgtggccc cttgctcgtc tacgaaaggc ccgttgtagc ggaaggcgcc gtccgcggtc
     4381 tccttgtaca gcgtgccgtc cgagttcatg tagtactggc cggccgtagc gaatgcgggc
     4441 gtgttcggca cgccgaagat gcccaacagg acgtttcgat tgtgcatgtt ggtcgcgacg
     4501 ggcgagtacc cggcggccga gtagaagtcg gtttgcgaaa gcgcgctcgg atccgcgtaa
     4561 cccgcgagcc tgaagtcgcg ctcgacggcg aagatctcgt cgcgcttcga acgctcgaag
     4621 ccgagcatca cgttgccctt gttgtcgaat gcgttcgtgc cgagcacgac ggagtaacga
     4681 aactccatgc cgtcgccttc cgctgtctgg ccgtactgcg tgtcgacgtc gacgccctcg
     4741 aagtcgcgct tcgttatgaa gttgacgacg cccgcgacgg cgtcggcgcc gtagacggac
     4801 gatgcgccgc ccgtgatcgt ttcgactcgc gcgatcgcgg ccgccgggat cgtgttgatg
     4861 ctcaccgaca tgccggcgtt gatcggcatc gcgcggcggc cgtcgagcag ca
//
LOCUS       DASSNN010000054         3754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1525289, whole genome shotgun sequence.
ACCESSION   DASSNN010000054 DASSNN010000000
VERSION     DASSNN010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525289"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VFL84_00950"
     CDS             <1..574
                     /locus_tag="VFL84_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HET7607217.1"
                     /translation="LRAFTPRDQKAVRSAAETMRPNPSFSIEQVITELGVGEALVSLL
                     DEKGRPGVTERALVLPPGSQLGPIPDSERARLLAASSLAGVYDKAVDRESAYEMLKGR
                     AEAPPSAERSPSPRQREAPAPRPPQSPRRADGGSLASDILFGRRGPRGGRQSKGLIEA
                     MANSAARSMGSQAGRALIRGVLGSLLGGRR"
     gene            complement(904..1257)
                     /gene="ssrA"
                     /locus_tag="VFL84_00955"
     tmRNA           complement(904..1257)
                     /gene="ssrA"
                     /locus_tag="VFL84_00955"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1333..1803)
                     /gene="smpB"
                     /locus_tag="VFL84_00960"
     CDS             complement(1333..1803)
                     /gene="smpB"
                     /locus_tag="VFL84_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011370832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET7607218.1"
                     /translation="MTKKEKKEQSSIAQNKKARFDYFIEDRLEAGLALQGWEVKSMRA
                     GKAQLTDSFVIFRDGEAWLLGSHVTPLSTVSTHVVAEPKRTRKLLMNRREIDRLIGLV
                     ERKGYTLIALELYWSKNRVKVAVGLAKGKKQHDKRDTEKDRDWKRDKARALKAH"
     gene            1860..2300
                     /locus_tag="VFL84_00965"
     CDS             1860..2300
                     /locus_tag="VFL84_00965"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HET7607219.1"
                     /translation="MPTVKRSARVPYTTEQMFDLVNDIEQYPKFLHWCRGARIDLKQG
                     NTVEATLDIGVLGFHQSFRTRNTLERPKRIGIDLVSGPFRRLRGEWRFVAAPDRGSDI
                     SLTLTFEVTLSPFGLVFTRVFEDIAGSQMKAFVDRANQLYAAKT"
     gene            complement(2310..2630)
                     /gene="bamE"
                     /locus_tag="VFL84_00970"
     CDS             complement(2310..2630)
                     /gene="bamE"
                     /locus_tag="VFL84_00970"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HET7607220.1"
                     /translation="MRFRGLVIAAAAVALTACVYRIDIQQGNLLDEDDINQVDLGMTR
                     SQVQFVLGTPMISDSFHRDRWDYAYYYRRGRSPDADRRWIVVYFENDRVQRIDRDLEL
                     QPES"
     gene            2703..3125
                     /gene="fur"
                     /locus_tag="VFL84_00975"
     CDS             2703..3125
                     /gene="fur"
                     /locus_tag="VFL84_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HET7607221.1"
                     /translation="MAVEGQDLRKAGLKVTSPRLKVLEVLEHSRQRHLSAEAIYRALL
                     ENGEELGLATVYRVLTQFERAGLVSRHNFEGGQAVFELADRTHHDHMVCLETGRVTEF
                     YDERIERIQREIADKHDYDIEEHSLVLYVRPKKKPAPT"
     gene            complement(3122..>3754)
                     /locus_tag="VFL84_00980"
     CDS             complement(3122..>3754)
                     /locus_tag="VFL84_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET7607222.1"
                     /translation="KRTAEARTQFLAAARRLSGLRAKHAAALSREVTALLVELGMPHG
                     EFRVELTEKPAERADATGLERVEFAVKLNPGLPFGPLAKVASGGELSRVSLAVEVARS
                     GASPVTAFVFDEVDAGVGGRVADIVGRKLRQLSGARQVLCVTHLPQVASHGETHYRVV
                     KQTDGKVSRTEVRKLTSKERVEELSRMLGGVEVTARTRAHAAEMIDRAGR"
BASE COUNT          618 a         1244 c         1214 g          678 t
ORIGIN      
        1 gctgcgcgcg ttcacgccgc gcgatcagaa agcggtccgg tccgccgccg agacgatgcg
       61 gccgaatccg agcttctcga tcgagcaggt catcacggag ctcggcgtcg gcgaggcact
      121 cgtgtcgctg ctcgacgaga agggccggcc cggcgtcacg gagcgcgcgt tggtgctgcc
      181 accgggtagc cagctcgggc cgatcccgga ttctgagcga gcgcgtctgc tcgcggcctc
      241 gtcgctcgcg ggcgtttacg acaaggccgt cgatcgcgag tcggcctacg agatgctgaa
      301 gggccgcgcc gaagcgcccc cgtcggcgga gcgctcgccg tcaccgcggc agcgcgaggc
      361 gccggcgccg cgcccgccgc agtcgcctcg gcgcgccgac ggcggaagtc tcgcatccga
      421 cattctgttc ggccggcgag gcccgcgcgg cggccggcaa tcaaaagggc tcatcgaagc
      481 gatggcgaac agcgctgcac gttcgatggg ctcgcaagcc ggacgcgcgc tgatccgcgg
      541 cgtcctcggc tcgcttctcg gcggccgtcg ctagcgaatg atgctgggtt cctccggccc
      601 ggcggccgga ggaacgaatt ggtggaggcg gcgggacagc tgttccatct ctttcgagac
      661 gggctggact atgccttcgt ccggccaaga gcgccggacg cggggcgtgt tatccggatc
      721 gaaagcgacc cggatcctag tgcaggccct tgcagcgaca tcggcctgtc tatgagtctc
      781 tacagggcga gggaagttgg ccttcccgca cccctcggcg ttgccacgtg actctctcga
      841 gccacacagg gttcaccgat tgagccccgt tatcactttg ccctcgcggg caaaggcgac
      901 cattgcgtta atcgaacccg cgtccgcaag ccctctgcca tcagctctac atgcgtagct
      961 acgtctactg ttttaaccgg cggcaacccg acgagcaggg tacaccgacg gcgagtccgt
     1021 aagtttttag cggccgagtc ccggacttac ccggccgcga tcctgcgaag atgacgcctg
     1081 attaagccct tgcgggcctg gacgcgcagg cgcggctcca gtcagacggc ataagccggt
     1141 ttttaggcgg ctagtgcgta gttgtcgtcg ttggcaacta acagtttgca gactgtttta
     1201 cgaggtatct gcacctcggc atgcacctca ggtttcgcga cccacgtcga agccagtcgc
     1261 ccccaaagtc aggctctagt ttagggccgc acctcgccta tgcaagggcc tgcgtcgcag
     1321 cggctcggcg gctcagtgcg ccttcagcgc gcgcgccttg tcgcgcttcc agtcgcggtc
     1381 cttttccgtg tcgcgcttgt cgtgctgctt cttgcccttc gcgaggccga cggcgacctt
     1441 gacgcgattc ttgctccagt agagctcgag cgcgatcagc gtgtagccct tgcgctcgac
     1501 gaggccgatc agccgatcga tctcgcggcg attcatcagc aatttgcgcg tgcgcttcgg
     1561 ctccgcgacg acgtgcgtcg agacggtgct caacggcgtg acgtgcgagc cgagcagcca
     1621 ggcttcgccg tcgcgaaaga tcacgaagct gtcggtcagc tgcgctttgc cggcgcgcat
     1681 gctcttgacc tcccagcctt gcagcgcgag cccggcctcg aggcggtctt cgatgaaata
     1741 gtcgaagcgc gccttcttgt tctgcgctat ggagctttgt tcttttttct ccttcttggt
     1801 catacctgcg ccatgatcct agaatcacct gcaaaaaaca ccggccgacg gggaacctct
     1861 tgccaactgt caagcgcagc gcgcgtgttc cgtacacgac ggagcagatg ttcgatctcg
     1921 tcaacgatat cgagcagtac cccaagttcc tgcattggtg cagaggggcg cgcatcgatt
     1981 tgaagcaagg caataccgtc gaggcgacgc tcgacatagg cgtgctcggg ttccaccaaa
     2041 gcttcaggac gcgcaacacg ctcgagcggc cgaagcgcat cggcatcgat ctcgtctcgg
     2101 ggccgttccg ccgcttgcgc ggcgagtggc gtttcgtcgc cgcgccggat cgaggcagcg
     2161 acatctcgtt gacgctcacg ttcgaggtca cgctgtcgcc tttcggcctc gtcttcacga
     2221 gagtgttcga agatatcgcg gggtcgcaaa tgaaggcgtt cgtcgaccga gcgaatcagc
     2281 tctacgcggc gaagacctga ccgggcgcgt cagctctccg gttgcagctc gagatcgcgg
     2341 tcgatgcgct gcacgcgatc gttctcgaag tagacgacga tccagcgcct gtcggcatcc
     2401 ggcgaacggc cgcggcgata gtaataggcg tagtcccacc ggtcgcggtg gaagctgtcg
     2461 gagatcatcg gcgtgccgag cacgaattga acctggctgc gcgtcatgcc gaggtcgact
     2521 tggttgatgt cgtcttcgtc gagcaggttg ccctgctgaa tgtcgatgcg atagacgcaa
     2581 gcggtgagcg cgaccgcagc ggcagctatc acgaggcctc gaaatctcat gcgttgcggt
     2641 ggagcgccag cagggcttcg gtcataatcg gaccggcatc ataccggata gttctcgagc
     2701 gagtggcggt ggagggacaa gatcttcgca aagccggcct caaagtcacc tctcctcgtc
     2761 tgaaggtgct cgaggtcttg gagcacagcc gccagcggca tctgtcggcc gaggcgatct
     2821 atcgcgcgct gctcgagaac ggcgaggagc tcgggctcgc cacggtctac cgcgtgttga
     2881 cgcagttcga gcgggcaggg ctcgtgagcc ggcacaactt cgaaggcggg caggccgtct
     2941 tcgagctcgc cgatcggacg caccacgacc acatggtttg tctcgagacg ggccgagtaa
     3001 ccgagttcta cgacgagcga atcgagcgaa tccagcgcga gatcgcggac aagcacgact
     3061 acgacatcga agagcacagc ctcgtgctct acgtgcgccc caagaaaaag ccggctccga
     3121 cctagcgccc ggcccggtcg atcatctccg ccgcgtgcgc gcgcgtgcgc gccgtgactt
     3181 cgacgccgcc gagcatccgc gacaactcct cgacgcgctc cttgctcgtc agcttgcgca
     3241 cttccgtgcg gctcaccttg ccgtcggttt gcttcacgac gcgatagtgc gtttcgccgt
     3301 ggctcgcgac ttgcggcaga tgcgtgacgc acagcacctg gcgagctccc gacagctgcc
     3361 gcagcttgcg cccgacgatg tcggcgacgc gccctccgac gcccgcgtcg acctcgtcga
     3421 acacgaacgc ggtgaccggc gatgcgcccg agcgcgcgac ctcgaccgcc aggctcacgc
     3481 gcgacagctc gccgcccgat gcgaccttcg cgagggggcc gaacggcagc cccgggttca
     3541 gcttgaccgc aaactcgact cgctcgagcc ccgtcgcgtc ggcgcgctcg gcgggcttct
     3601 ccgtgagctc gacgcgaaac tcgccgtgcg gcatgccgag ctcgacgagc agcgcggtga
     3661 cttcgcggct caacgcggcc gcgtgcttgg cccgcaggcc gctcaggcgg cgcgcggccg
     3721 cgagaaactg cgttcgcgct tccgcggttc gctt
//
LOCUS       DASSNN010000055         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_12239, whole genome shotgun sequence.
ACCESSION   DASSNN010000055 DASSNN010000000
VERSION     DASSNN010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12239"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VFL84_00985"
     CDS             complement(<1..1023)
                     /locus_tag="VFL84_00985"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7607223.1"
                     /translation="MSMRFVIGSCVALLAAIAAPLASAHHAIVAKFDDTKTREVRGIV
                     TNVDWRNPHVHVFVNTAKDDGTLENWAVELESTVLLVRSGWAHDTLKPGDSVRASGPV
                     ARDGSRQIWGTRLALANGRQVYDVKDAPPTVSRSQQKPVPRGSDGKPLLGVVDASGGY
                     WAYPTSTVLQQNGANVRMDKDGVLARLSDAPRVAPFQPWALGLYEHRQRRHLADDPGF
                     LNCKNPGAVRQFQTPYGVQFLQDAPRKRVFVLIGGGNRNYRIIYMDGRDQKGQVQGDD
                     DNPLYYGRAVAHWEGDTFVVNTTGFNEDFWFTNGGLPHTNQLSLVERFTRTDFNTLRY
                     EVVVDDP"
     gene            complement(1020..1421)
                     /locus_tag="VFL84_00990"
     CDS             complement(1020..1421)
                     /locus_tag="VFL84_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607224.1"
                     /translation="MKRLMTIAAAAVLALAFMVPSSAQEGHPLKGSWLGEWKGNTVHG
                     DNILLILDWDGKAITGMINPGTDNIPLKSASLEPKGWVVHIEADGKDKDGSTVSYVID
                     GKIENLELPNRSIVGTWSSNKGRGMFTASRQ"
     gene            complement(1538..2668)
                     /locus_tag="VFL84_00995"
     CDS             complement(1538..2668)
                     /locus_tag="VFL84_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7607225.1"
                     /translation="MRAIAIVSLGLLAAVPAAAQPTAGEDSQHILTVDHYVPNRSIVP
                     SIQGETTQIYVRERVKPATIQRGAPRDDRVVLFVHGAGTPAEVAFDVPYSDYSWMAVL
                     AAAGFDAFSMDMTGYGRSTRPNVMNDVCNLSAEQQTALGRTPCQPSHDAQLTTIASDW
                     HDLDAVVEYIRKLRDVPRVSLVGWSMGGPRAGGYASRNPDMVAKLVLLAPAYSRDAGL
                     EPPQPNPGRGVAFNAQSHADFTANWDRQVGCANQYEPAAAAAVWRAMLESDPVGASWG
                     QGVRRAPNTTVWGFGKAAVERMQTPTLMVAGVHDKQVDPARVRDYFADLGAENKVLLD
                     LGCSSHNAMWESNHLLLFRASLEWLTAGTVQGMAQGVVRLGY"
BASE COUNT          457 a          961 c          947 g          495 t
ORIGIN      
        1 cgggtcgtcg acgactactt catatcgtaa cgtgttgaag tcggtgcgcg tgaagcgctc
       61 gacgagcgag agctggttcg tatgcggcaa tccgccgttc gtgaaccaga agtcctcgtt
      121 gaaccccgtg gtgttcacga cgaacgtgtc gccctcccag tgcgcgacgg cgcggccgta
      181 atagagcgga ttgtcgtcgt cgccctgcac ttgccccttc tgatcgcggc cgtccatgta
      241 gatgatgcgg tagttgcggt tgccgccgcc gatcagcacg aagacgcgct tgcgcggcgc
      301 gtcctgcagg aattgcacgc cgtacggcgt ctgaaactgg cgcacggcgc ccgggttctt
      361 gcaattcaag aagcccggat cgtcggcgag atggcgccgc tggcggtgct cgtacaaccc
      421 tagagcccac ggttgaaacg gagcgacgcg cggcgcatcg gagagccgcg cgagcacgcc
      481 gtctttgtcc atacggacgt tggcgccgtt ctgctggagc accgtgctcg tcggatacgc
      541 ccaatacccg ccgctcgcat cgacgacgcc gagcagcggc ttgccgtccg agccgcgcgg
      601 cacgggtttt tgctgcgagc gcgacacggt aggcggcgcg tccttgacgt cgtagacctg
      661 acggccgttc gcgagcgcga ggcgcgtgcc ccagatctgg cgcgagccgt cgcgcgcgac
      721 ggggccgctc gcgcgcacgg aatcgccggg cttcaacgta tcgtgcgccc agccgctgcg
      781 aacgagcagc accgtgctct cgagctcgac ggcccagttc tcgagcgtgc cgtcgtcctt
      841 cgcggtgttg acgaagacgt gcacgtgcgg gttgcgccag tccacgttcg tcacgatgcc
      901 acgcacttcg cgggtcttgg tgtcgtcgaa cttcgcgacg atcgcgtgat gcgcggacgc
      961 gagcggcgcc gcgatggcgg cgagcaaagc gacgcacgag ccgatgacga aacgcatgct
     1021 cattgccggc tcgccgtgaa catgccgcgg cctttgttgc tcgaccatgt gccgacgatc
     1081 gagcggttcg gcagctcgag gttctcgatc ttgccgtcga tgacgtagct cacggtgctg
     1141 ccgtctttgt ccttgccgtc ggcctcgatg tggacgaccc agcctttcgg ctcgagcgac
     1201 gccgacttca gcgggatgtt gtcggtgccc gggttgatca tgcccgtgat ggccttgccg
     1261 tcccagtcga ggatcaacag gatgttgtcg ccatggaccg tgttgccctt ccattcgccg
     1321 agccacgagc ctttgagcgg atgcccttcc tgcgcgctgc ttgggaccat gaaagccagc
     1381 gccaaaaccg ccgccgccgc gatcgtcatt agtcttttca tcaaccttct ctcggcctta
     1441 ctagccatcc agtgtaccgg cgccgcgcgg tgcctcaata aagaattgtc ggtccgcccg
     1501 ctgcaagcgc atgccgcgcc gcaatgtgag cggcgagtca gtaaccgagc cggacgacgc
     1561 cctgcgccat cccttgcacg gtccccgccg tcagccattc gagcgaggcg cgaaacaaca
     1621 gcaagtgatt cgactcccac atcgcgttgt gagacgagca gccgagatcg agcaagacct
     1681 tgttctcggc gccgagatcg gcgaagtaat cgcgcacccg cgcgggatcg acctgcttgt
     1741 cgtggacgcc ggcgaccatc agcgtcggcg tctgcatgcg ctcgacggcc gctttgccga
     1801 agccccagac ggtcgtgttc ggcgctcgcc gcacgccctg tccccacgac gcgccgaccg
     1861 ggtccgactc gagcatcgcg cgccacacgg ccgcggcggc ggccggctcg tattgattcg
     1921 cgcagccgac ctggcgatcc cagttcgcgg tgaagtcggc gtgcgattgc gcgttgaagg
     1981 cgactccgcg cccgggattc ggctggggag gctcaaggcc cgcatcgcgc gagtacgcgg
     2041 gcgcgagcag cacgagcttc gccaccatgt ccggattgcg cgatgcgtag ccgcccgcgc
     2101 gcggcccgcc catcgaccaa ccgacgaggc tcacgcgcgg cacgtcgcgg agcttgcgga
     2161 tgtactcgac gacggcgtcg aggtcgtgcc agtccgaagc gatggtcgtg agctgcgcgt
     2221 cgtgcgaggg ttgacaaggc gtgcgtccga gcgccgtttg ctgctcggcc gagagattgc
     2281 agacgtcgtt catgacgttc ggccgcgtcg agcggccgta acccgtcatg tccatcgaga
     2341 acgcatcgaa gccggccgcg gcgaggaccg ccatccagct gtagtcggag tacggcacgt
     2401 cgaacgcgac ctcggccgga gtcccggcac cgtgcacgaa cagcacgacg cggtcgtcgc
     2461 gcggagcgcc gcgctgaatc gtggccggct tcacgcgctc gcgcacgtag atctgcgtcg
     2521 tctctccttg aatggaagga acgatcgagc ggttcggcac gtagtggtcg accgtcagaa
     2581 tatgctggct gtcctcgccg gccgtcggtt gagcggcggc gggaaccgcc gcgagcaaac
     2641 ccaaggacac gatcgcgatc gcgcgcacgt taggcccccc taagaaatcg agcccaatcg
     2701 tcgcatgacg aacgggacag cgcctagctt tgcggtgcgg cggccgtcat cagccgcgcg
     2761 agcgcggcat cgtcgtcgcg ccccgacccc tgcagctgcg cgacgacgcc agcgcggtcc
     2821 gcctcggagc ggttctggct cagcttgaac ttgccctcga
//
LOCUS       DASSNN010000056         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1020547, whole genome shotgun sequence.
ACCESSION   DASSNN010000056 DASSNN010000000
VERSION     DASSNN010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020547"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2175
                     /locus_tag="VFL84_01000"
     CDS             <1..2175
                     /locus_tag="VFL84_01000"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET7607226.1"
                     /translation="WELAIRKLRAGLMPPADGGPTLERDERQDLAAWLEREIDKDAEP
                     YLPAPGLHRLNRTEYTNAIRDLLGLNIDATKFLPSDDSSHGFDNMAGTLTTSPALMEA
                     YLSAAGKISRLSVGTETAPTLAVFDAPHDTSQNAYVEGLPFGTRGGMLIEHEFPADGN
                     YTFTVKGMTGYFTAVLGNVKGEQLEISIDGTRVYLYDWDKEIATQEGNGGRSPEIPIK
                     AGFHKVGVTFLATSDLPDTGLNKSFVRTMNSPGSISGYTFYPHVGQVFIEGPFQGQVA
                     KSTPSRDRIFECYPAESSQEAECARKIISTLAGKAFRRPATDADIETMMTFFDAGRDE
                     GGAFDYGIEAVVQRLLADPEFIYRSELEPANVAPGQPYRISDLELASRLSFFLWSSIP
                     DRELVELASQGRLHERKVLEQQVARMIADERSNAFVENFTGQWLNVRGMQASEPVVEL
                     FPDFDSTLRDAMRREIELFFGSIIQEDRSILDLLTADYTFVNERLAKHYGIPDVYGSQ
                     FRRVELGPELDARRGLLGKGALLTITSDVARTSPVKRGKWFLETFLDISPPDPPPGVN
                     TTLPVEKDAAPKTLRARMEAHRTDPNCAACNKIFEPMGLAMENFDGVGAWRTLDAGQP
                     IDTTGVTNDGASLDGVKSLRDYTMSNADLFAQSVTEKLLTYAIGRGLEYEDMPLVRKI
                     THDAADDDYKFSSLLMGVVESPAFTMNVKSAAAANTTAANTE"
     gene            2180..>2572
                     /locus_tag="VFL84_01005"
     CDS             2180..>2572
                     /locus_tag="VFL84_01005"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET7607227.1"
                     /translation="MFITKKHLSRRTLLKGAGVALGLPLLDSMFPAATAFAQSAAAPP
                     RRFFAGFVPHGAAPGYWVPEKEGALPAKLPFIWEPLEPFRQSLTVLTGLHSTSSEPPP
                     GETGADHWVAAAFLCADKPKKTAGADVRA"
BASE COUNT          465 a          882 c          842 g          383 t
ORIGIN      
        1 tgggagctcg ccatccgcaa gctgcgcgcg ggcctgatgc cgcccgccga cggcgggccc
       61 acgctcgagc gcgacgagcg ccaggatctc gccgcgtggc tcgagcgcga aatcgacaag
      121 gacgccgagc cgtatctgcc cgccccgggc ctgcatcggc tgaaccgcac cgagtacacg
      181 aacgcgatcc gcgatctgct cggcctcaac atcgacgcga cgaagttcct gccctccgac
      241 gactcgagcc acgggttcga caacatggcc ggcacgctga cgacgtcgcc ggcgctgatg
      301 gaggcgtatc tgtcggcggc gggcaagatc agccggctta gcgtgggcac ggagacggcg
      361 ccgacgctgg ccgtgttcga cgcgccgcac gacacgtcgc agaacgcgta cgtcgaaggg
      421 ctgccgttcg gcacgcgcgg cggcatgctg atcgagcacg agttccccgc ggacggcaac
      481 tacacgttca cggtcaaggg aatgacgggc tatttcacgg ccgtgctcgg caacgtcaaa
      541 ggcgagcagc tcgaaatatc gatcgacggc acgcgcgtct atctctacga ctgggacaaa
      601 gagatcgcga cacaggaagg caacggcggg cgctcgccgg agattccgat caaggcgggc
      661 ttccacaaag tcggcgtcac gttcctcgcg acgagcgact tgcccgacac cgggctcaac
      721 aagtcgttcg tgcggacgat gaactcgccg ggctcgatct cgggctacac gttctacccg
      781 cacgtcggcc aggtcttcat cgaagggccg ttccaaggcc aagtggccaa gagcacgccg
      841 agccgcgaca ggatcttcga gtgctacccg gccgagagca gccaagaggc cgagtgcgcg
      901 cgcaagatca tctcgacgct cgcgggcaaa gcgttccgcc gtcccgcgac cgatgcggac
      961 atcgaaacga tgatgacgtt cttcgacgcc ggccgcgacg aaggcggcgc gttcgattac
     1021 ggcatcgagg ccgtcgtgca gcggctgctc gcggatcccg agttcatcta tcgctcggag
     1081 ctcgagccgg ccaacgtcgc gccgggccag ccgtatcgaa tcagcgatct cgagctcgcc
     1141 tcgcgcctgt cgttcttcct ctggagcagc atccccgacc gagagctcgt cgagctcgct
     1201 tcgcagggcc gcctgcacga gcggaaagtc ctcgagcagc aagtcgcgcg catgatcgcc
     1261 gacgagcgtt cgaacgcgtt cgtcgagaac ttcaccggcc agtggctcaa cgtgcgcggc
     1321 atgcaggcga gcgagcccgt cgtagagctg ttccccgatt tcgacagcac gctgcgcgac
     1381 gcgatgcggc gcgagatcga gctcttcttc ggcagcatca ttcaggaaga ccgcagcatc
     1441 ctcgacctgc tgacggccga ttacacgttc gtcaacgagc ggctcgcgaa gcactacggc
     1501 attcccgacg tctacggctc gcagttccgc cgcgtagagc tcggacccga gctcgacgcg
     1561 cggcgcgggc tgctcggcaa gggcgcgctg ctgacgatca cgtccgacgt cgcgcgcacg
     1621 tcgccggtca agcgcggcaa gtggttcctc gagacgttcc tcgacatcag cccgcccgac
     1681 ccgccgcccg gcgtcaatac gacgctgccc gtcgagaagg acgcggcgcc gaaaacgctg
     1741 cgcgcgcgca tggaagcgca tcgcaccgat ccgaactgcg cggcgtgcaa caagatcttc
     1801 gagccgatgg ggctcgcgat ggagaacttc gacggcgtcg gcgcgtggcg cacgctcgat
     1861 gccggccagc cgatcgatac gaccggcgtc acgaacgacg gtgcgagctt ggacggcgtc
     1921 aagagcctgc gcgactacac gatgagcaac gccgacctgt tcgcgcaatc ggtcaccgag
     1981 aagctgctga cgtatgcgat cggccgcggc ctcgagtacg aggacatgcc gctggtccgc
     2041 aagatcacgc acgacgcggc cgacgacgac tacaagttct cgtcgctgct catgggcgtc
     2101 gtcgaaagcc ctgcgttcac gatgaacgtg aagagcgcag ccgcggcgaa caccaccgcg
     2161 gcgaatacgg agtagagcga tgttcatcac gaaaaagcac ttgtcgcgac gcacgctgct
     2221 caaaggcgcc ggtgtggcgc tcggcttgcc gttgctcgac tcgatgtttc cggccgcgac
     2281 ggcgttcgcg caatcggccg cggctccgcc tcgccgcttc ttcgcgggct tcgtcccgca
     2341 cggcgccgcg cccgggtatt gggttcccga gaaggaaggc gcgctgcccg ccaagctgcc
     2401 gttcatctgg gagccgctcg agccgtttcg gcagagcctc acggtcctga cgggcctgca
     2461 ctcgacttcg tccgagccgc cgcccggcga aacgggcgcg gaccactggg tcgccgcggc
     2521 gttcttgtgc gccgacaagc cgaagaagac cgcgggcgcc gacgttcgcg cc
//
LOCUS       DASSNN010000057         2487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_888714, whole genome shotgun sequence.
ACCESSION   DASSNN010000057 DASSNN010000000
VERSION     DASSNN010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888714"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFL84_01010"
     CDS             <1..276
                     /locus_tag="VFL84_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607228.1"
                     /translation="SALDPTQVVSPLSTELGAQQSYDVFDLTGSWNATSKTAVRFGIT
                     NLFDTPPVWTGGRTAADNHPSDGSGQTEAGYYDLLGRQFYLGVSTTF"
     gene            complement(312..1985)
                     /locus_tag="VFL84_01015"
     CDS             complement(312..1985)
                     /locus_tag="VFL84_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006321819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HET7607229.1"
                     /translation="MTAVETKHSEVEAIKLRSRLLRGTLAAGLEDPVTATISDDDAQI
                     SKFHGVYRQDDRDVRAERAAQKLEPAYIFMVRLRVPGGVLTSAQWLAVDEIARTYGNG
                     AARLTTRQSIQLHGVLKRNLKAAMRAIGDAGLTTIAACGDVNRNVVCTPLPEQSTLHG
                     AVYEHAMSVVRLLTPQTGAYREIWMDEPGRANEREPLYGAAYLPRKFKVGFVVPPGND
                     IDVFAQDLGFIAVQREGVLAGFNVAVGGGLGMTHGDAKTYPRLAEVIGFCLPEEVLGI
                     VEHVVGIQRDYGDRGDRSHARLKYTIDDRGTPWFKQELERRSGFALEAAVPVKLLHSG
                     DRFGWQRGEDGRSHLTLLVPSGRVRDDERRRLRTGINAIAKIHSGDFRLTGNQNLIVA
                     NVPPERVEALQRIVSEHGLDTHERLAPLRRNAMSCVALPTCGLAMAEAERYLPELLAK
                     LEPLLARHGLEDEDLVLRLTGCPNGCARPYLAEIALVGKAPGRYSLFLGGSVTGERLN
                     RLYRQNLSEQEILGVLDGLFGEFARGRLKGERFGDFVQRRGLVGAEAAR"
     gene            complement(1993..>2487)
                     /locus_tag="VFL84_01020"
     CDS             complement(1993..>2487)
                     /locus_tag="VFL84_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607230.1"
                     /translation="SIYVEPNDRFRLPADSALPIIMIGAGTGVAPYRAFVQHRREHGA
                     RGENWLIYGDRNMRSDFLYQIEWLRHLRDGTLNRMTVAFSRDQERKIYVQHRIAEEAR
                     EIYAWLERGAHVYVCGEAENMAPAVHAALRGAIQSAGGRTREAAEAYLQELKVSRRYQ
                     RDVY"
BASE COUNT          424 a          834 c          792 g          437 t
ORIGIN      
        1 agcgcgctcg acccgacgca agtcgtgtcc ccgctatcga ccgagctcgg ggcgcagcag
       61 tcgtacgacg tgttcgacct gaccggctcc tggaacgcca cgtccaagac ggcggttcgc
      121 ttcggaatca cgaacttgtt cgacaccccg cccgtttgga cgggcggccg gacggccgcc
      181 gacaatcatc caagcgatgg ctcgggtcag accgaggcgg ggtactacga cctcctcggg
      241 cggcagttct atcttggcgt cagcacgact ttctagtcgg tcttttcgct gcaagcggac
      301 ggaggccgcg cctatcgcgc ggcctctgct cccaccagac ccctccgctg gacgaagtcg
      361 ccgaagcgct cgccctttag ccggccgcga gcgaattcgc cgaagagtcc gtcgagcacg
      421 ccgaggatct cctgttccga cagattctgg cgatacagcc gattgagccg ttcgccggtg
      481 acgctgccgc cgaggaagag gctgtagcgg cccggggctt tgccgaccag agcgatctcg
      541 gcgagataag gccgcgcgca gccgttcgga cagcccgtga ggcgcagcac gagatcctcg
      601 tcctcgagcc cgtgcctcgc gagcaaaggc tcgagcttgg cgagcagttc gggcagatag
      661 cgctccgctt cggccatggc caaaccgcag gtcggcaggg cgacgcagct catcgcgttg
      721 cgccgcaaag gcgcgagccg ctcgtgggta tcgagcccgt gctcgctgac gatgcgctgc
      781 agcgcctcga cccgctcggg cgggacgttc gccacgatga gattttggtt gccggtcaag
      841 cgaaaatcgc cgctatgaat tttcgcgatg gcgttgatgc cggtacggag ccgccggcgt
      901 tcgtcgtccc ggactcggcc cgacggaacg agcaaggtca gatgcgagcg tccatcctct
      961 ccgcgctgcc aaccgaaacg atcgccgctg tgcagcagct tcacgggcac ggcggcttcg
     1021 agcgcgaagc ccgatctccg ctcgagctcc tgcttgaacc acggcgtgcc gcggtcgtcg
     1081 atggtgtact tgagcctcgc atggcttcgg tcgccgcgat cgccgtaatc gcgctggatg
     1141 ccgaccacgt gctcgacgat gcctagcacc tcttccggca ggcagaaacc gatgacttcg
     1201 gccagacgcg gataggtctt cgcatcccca tgcgtcatgc cgagaccgcc gccgacggcg
     1261 acgttgaagc ccgcgagcac gccttccctc tgcacggcga tgaagccgag atcctgcgcg
     1321 aacacgtcga tgtcgttgcc cggcggcacg acgaagccga ccttgaactt gcgcggcaga
     1381 taagcggcgc cgtacagagg ctcccgctcg ttggccctgc ccggctcgtc catccagatc
     1441 tcgcgatagg cgcccgtctg cggcgtcagc agccgcacga cgctcatcgc atgctcgtac
     1501 acggcgccat gcagcgtcga ctgctccggc aagggcgtgc agacgacgtt gcggttgacg
     1561 tcgccgcagg ccgcgatcgt cgtgaggccc gcgtcgccga tcgcccgcat ggcagccttt
     1621 agattgcgct tcaaaacgcc atgcagctga atcgactgac gggtcgtcaa acgagccgcg
     1681 ccgttgccgt aggttcgggc gatctcatcg acggccagcc actgcgcgga agtgagcact
     1741 cctcccggaa cgcggaggcg caccatgaag atgtacgcgg gctcgagctt ctgcgccgcg
     1801 cgttcggcgc ggacgtcccg gtcgtcctgg cgatagacgc cgtggaactt ggaaatctgc
     1861 gcgtcgtcgt cgctgatcgt ggccgtgacg gggtcttcga ggcctgccgc aagcgtgccg
     1921 cgcaacagcc ggctgcgaag cttgatggcc tcgacttccg aatgtttcgt ctcgacagcc
     1981 gtcatgctct tctcagtaaa cgtcccgctg ataacggcgg ctaaccttta gctcttgcaa
     2041 ataggcttcg gccgcttccc gcgtgcgtcc cccggcgctt tgaatggccc ctctcaacgc
     2101 cgcgtgcacg gccggagcca tgttctcggc ctctccgcaa acataaacgt gcgcgccgcg
     2161 ctcgagccag gcatagattt cgcgggcctc ctcggcgatt cgatgctgca cataaatctt
     2221 gcgctcctga tcgcgcgaga acgcgaccgt catgcgattc agcgtgccgt ccctgaggtg
     2281 gcgcagccat tcgatttgat agaggaaatc ggaacgcatg ttgcgatcgc cgtaaatgag
     2341 ccaattctcg ccgcgcgcgc cgtgctcgcg gcgatgctgc acgaaagcgc gatagggcgc
     2401 tacgccggta ccggcgccga tcatgatgat gggcagcgcg gaatcggccg gcaggcggaa
     2461 acgatcattg ggttcgacat agatcga
//
LOCUS       DASSNN010000058         3302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1399585, whole genome shotgun sequence.
ACCESSION   DASSNN010000058 DASSNN010000000
VERSION     DASSNN010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399585"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VFL84_01025"
     CDS             complement(<1..733)
                     /locus_tag="VFL84_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7607231.1"
                     /translation="MRSTHVVIALAALVCTAAAFAQAPDPRAVFTNACAACHGDDGRG
                     RSSSELGFDTPLPDFTDCDFAVREPNADWYAIVHRGGPVRGFDRMMPAFGEALAPEEI
                     EAALERIRSFCTDARWPRGELNVPRALFTEKAYPEDEAVITTTLIGEGNDAITHEFVW
                     EQRFGPLNQMELILPITRADLGDSQGWQSGTGDLGIGVKHTLRHSLERGAILAVGGEL
                     VLPTGDEDKGFGNESTVLEAHVLYDK"
     gene            811..2412
                     /locus_tag="VFL84_01030"
     CDS             811..2412
                     /locus_tag="VFL84_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607232.1"
                     /translation="MRLRIVRICVAAALLAGAQARAQDEASAFVPSSQCIACHSQVTA
                     ASGEDVSIGPHWRATIMANSARDPYWHAAVRREALDHPALQTDIEDTCSTCHMPMARF
                     DAAAAGRKGQVFANIAAAAPQHELAFDGVSCTVCHQISAENLGSHASFDGGFVIETPS
                     GGRVAFGPHDVDAGRQALMRSAATFKPSMSTHIQQSELCATCHTLFTTARDDAGREIG
                     TLPEQVPYQEWLHSDYRATNSCQSCHMPAVAAESPITSVLGQPRPNVSQHTFLGGNAF
                     MLDILRRYRGELGVKALPQELEASAAATRAFLSTRAATLSIAAPQRTAAGVELTVDVK
                     STTGHKLPTAYPSRRAWLHVVVRDAAGRAVFESGAVRPDGSIAGNDNDADAQRFEPHH
                     EVVTSQDEVQIYEAIMVDSRDALTTGLLNAVRFVKDNRLLPRGFDKRTAEPDFAVRGA
                     AADDADFIGGADRVRYRIPLAAGLSGPLTVEAELLYQSISYRWAENLRAYDAEEAQRF
                     GRYYAENAAASAAVIATAGATIPAR"
     gene            2484..2945
                     /locus_tag="VFL84_01035"
     CDS             2484..2945
                     /locus_tag="VFL84_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET7607233.1"
                     /translation="MRLKVSLSIALVMLCVFGIGRAAPPPALQPVDGCVIWVDFWASW
                     CVPCRRSFPWLNSMHRKYGGEGLQVIGVNLDKDRALADAFLAEVPAEFALRFDPTGAL
                     AKQYGVQAMPSSFLIDADGNVLASHAGFRTADSADYEKAITDALSKPRLQR"
     gene            2957..3181
                     /locus_tag="VFL84_01040"
     CDS             2957..3181
                     /locus_tag="VFL84_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005214962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HET7607234.1"
                     /translation="MKLAGKGMVALFVTTAIALSACARVEPWDRDVLARPDMQIVADP
                     IESAADEHIYFSKEASSGGQGFGGGGCGCN"
BASE COUNT          520 a         1099 c         1147 g          536 t
ORIGIN      
        1 gcttgtcgta gagcacgtgc gcctcgagaa ccgtgctctc gttgccgaat cctttgtcct
       61 cgtcgccggt cggcaatacg agctcgccgc ccacggcgag gatcgcgccg cgctcgaggc
      121 tatgccgcag cgtgtgcttg acgccgatgc cgagatcgcc ggtgccgctc tgccagcctt
      181 ggctgtcgcc gagatcggcg cgcgtgatcg gcaggatcag ctccatctga ttcaagggcc
      241 cgaagcgctg ctcccagacg aactcgtgcg tgatcgcgtc gttaccttcg ccgatcagcg
      301 tcgtcgtgat gacggcctcg tcctcgggat acgctttctc cgtgaacagc gcgcgcggca
      361 cgttgagctc gccgcgcggc caacgcgcgt cggtgcagaa gctgcgaatg cgctcgagcg
      421 cggcttcgat ctcctcgggc gcgagcgctt cgccgaacgc cggcatcatg cggtcgaagc
      481 cgcgcaccgg gccgccgcga tgcacgatcg cgtaccagtc ggcgttcggc tcgcggaccg
      541 cgaagtcgca gtcggtgaag tcgggcagcg gagtgtcgaa gccgagctcg ctcgagctgc
      601 ggccgcggcc gtcgtcgccg tgacacgctg cgcacgcgtt cgtgaagacg gcgcgcgggt
      661 cgggcgcttg cgcgaacgcc gccgcggtgc agacgagcgc cgcgagcgcg atgacgacat
      721 gagtcgagcg cacgagcttg cgactccgat tgagtaaacg acgcgccgca gtatagtgac
      781 ggcccgaccg ccgaaaaagc tcggggaatc atgcgcctgc gaatcgttcg tatttgcgtt
      841 gcagcggcct tgctcgcggg cgcgcaggcg cgggcgcaag acgaagcgag cgcgttcgtt
      901 ccttcctcgc agtgcatcgc ctgtcactcg caggtcacgg ccgcgagcgg cgaggacgtc
      961 tcgatcggtc cccattggcg cgcgacgatc atggcgaact cggcgcgcga tccttactgg
     1021 cacgcggccg tgcggcgcga ggcgctcgat catccggccc tgcagaccga catcgaggac
     1081 acgtgctcga cgtgccacat gccgatggcg cgcttcgacg ccgccgcagc cggccggaag
     1141 gggcaggtgt tcgcgaacat cgcggccgcc gcgccgcagc acgagctcgc gttcgacggc
     1201 gtctcgtgca cggtctgtca ccagatctcg gcggaaaatt tgggtagtca cgcgagcttc
     1261 gacggcgggt tcgtgatcga aacgccgagc ggcggccgcg tcgcgttcgg cccgcacgac
     1321 gtcgacgcgg gacggcaggc cctgatgcgc tcggccgcga ccttcaagcc gagcatgagc
     1381 acgcacatcc agcagtcgga gctctgcgcg acgtgtcaca cgctgttcac gacggcacgc
     1441 gacgatgcgg gccgggaaat cggcacgctg cccgagcaag tgccgtatca agagtggctg
     1501 cacagcgact atcgcgcgac gaatagctgc cagagctgtc acatgcccgc ggtcgccgcc
     1561 gaatcgccga tcacgtccgt gctcggccaa cctcggccga acgtctcgca gcacacgttc
     1621 ctcggcggca acgcgttcat gctcgacatc ttgcggcgct atcgcggcga gctcggcgtg
     1681 aaggctctgc cgcaggagct cgaggcgtcg gccgccgcga ctagggcgtt cttgagcacg
     1741 cgtgcggcga cgctgtcgat tgcggcaccg cagaggacgg ccgccggcgt cgagctcacc
     1801 gtcgacgtga agagcacgac gggccacaag ctgccgaccg cttatccgtc gcggcgtgcg
     1861 tggctgcacg tcgtcgtgcg cgatgcggcg ggtcgcgccg tcttcgaatc cggcgcggtc
     1921 cggccggacg gcagtattgc cggcaacgac aacgacgccg acgcgcagcg cttcgagccg
     1981 catcacgagg tcgtgacttc gcaggacgag gtccagatct acgaggcgat catggtcgat
     2041 tcgcgcgatg cgctgacgac ggggttgctc aacgccgtgc gcttcgtcaa ggacaaccgc
     2101 ctgctgccgc gcggcttcga caagaggact gccgagcccg atttcgcggt tcggggcgcc
     2161 gcggccgacg acgccgattt catcggcggc gccgatcgcg tgcgctaccg gatcccgctc
     2221 gctgcgggac tgtccggtcc gctgaccgtc gaagcagagc ttctgtacca atcgatttcg
     2281 tatcgttggg ccgaaaattt gcgcgcgtac gacgccgagg aggcgcagcg cttcggccgt
     2341 tactacgcgg agaacgcggc cgcgtcggcc gcagtcatag cgacggcggg ggccacgatc
     2401 ccggcgcgct gattgcggcg accggttttg cggccggcgg ccgtcctacg gacgccgtcg
     2461 ctcgatccgg acaaggagga ctcgtgcgct tgaaggtttc gttgtcgatc gcccttgtca
     2521 tgctctgcgt cttcgggata ggacgcgcgg cgccgccgcc ggcgctgcaa cccgtcgacg
     2581 gctgcgtgat ctgggtcgac ttctgggcat cctggtgcgt gccgtgccgg cgctcgtttc
     2641 cgtggctgaa ctcgatgcat cgcaagtacg gcggcgaggg cttgcaggtc atcggcgtga
     2701 atctcgacaa ggatcgagcg ctcgccgacg cgttcctcgc cgaagtcccc gccgaattcg
     2761 cgctgcgctt cgatcccacc ggcgcgctcg cgaagcaata cggcgttcag gcaatgccga
     2821 gctcgttttt gatcgacgcc gacggcaacg tgctcgcgag ccacgcgggc tttcgcacgg
     2881 cggactcggc cgactacgaa aaagcaatta cggacgcgct ttcgaagccg cgcctgcagc
     2941 gttaggaggg agcacgatga agctcgctgg caagggaatg gtcgccctgt tcgtgacgac
     3001 ggcaatcgcg ctctctgcgt gcgcgcgcgt cgagccgtgg gatcgcgacg tgctcgcgcg
     3061 gcccgacatg cagatcgtcg cagatccgat cgagtcggcg gccgacgagc acatctattt
     3121 cagcaaggaa gcctcgagcg gtggccaagg cttcggtggc ggaggttgcg gatgcaactg
     3181 aaacgccgaa acgccgcgct gcgcgagatc ggcacgccgc tcgcggcggc cacgtgcgcg
     3241 ttgctggggc acgcagcgcc tgcgagcgtg caggcgcagg agctgatgcc ctgggacatc
     3301 ga
//
LOCUS       DASSNN010000059         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_637518, whole genome shotgun sequence.
ACCESSION   DASSNN010000059 DASSNN010000000
VERSION     DASSNN010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637518"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /locus_tag="VFL84_01045"
     CDS             complement(<1..1489)
                     /locus_tag="VFL84_01045"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HET7607235.1"
                     /translation="MNGKKIVGLGYNGLAKAVYVACLGMAGQQALAQEPVEEVTVTGS
                     RLIQSGVNTPTPVTAVSAAELQTMAPTTLIESLSQLPVFYNDLTSQQAVGGSVASGGS
                     NLNLRGLESRRTLVLMDGHRLGPSNKFGTVDVSVIPEMLVSSVEAVTGGASAAYGADA
                     VAGVVNFRLDRNYTGIKYQFQAGQTTESDGESYKAGLAWGGDVGEKGHIIVSGEWWEK
                     EGIRDFESLLARSDYLDLKAQVTNPDPVNGPDFLARPFVSPTNFTEGGLLLQPGSAVD
                     HIEFLPDGSGNFRPLPFSGVGLLSGGCNCQARPTLEYGIDRDYEVDTPADRGVAFVHY
                     DHELSDRNSFYLETLLANSSQANVWQSAALLGPWGGRIYADNPFLPAPLAATLLSEGR
                     QFAGFGIFTPNLEGNPFDTAELIAKNRYGQFVAGFAHDMSEDFLRGNWVLDSYVQYAQ
                     NRQETVVPGGIRTDLLPIAMDVVTGPGGQPVCRVTLFISGPFDDCV"
BASE COUNT          352 a          639 c          651 g          393 t
ORIGIN      
        1 gcacgcagtc gtcgaagggt ccggagatga acagcgtcac gcggcacacg ggttggccgc
       61 ctgggccggt cacgacatcc atcgcgatgg gcaagaggtc cgttcgaatg cctccgggaa
      121 cgaccgtttc ctgccggttc tgagcgtact gcacgtagct gtcgagcacc cagttgccgc
      181 gcaggaagtc ctcgctcatg tcgtgagcga agccggcgac gaactggccg tagcgattct
      241 tcgcgatcag ttcggccgta tcgaacgggt tgccctcgag gttcggcgtg aagatcccga
      301 agcccgcgaa ttgccggcct tcgctcagca acgtcgccgc gagcggcgcc ggtaagaacg
      361 gattgtcggc gtagattctg ccgccccacg gtccgagcaa cgcggcgctc tgccagacgt
      421 tggcttgcga ggaattcgcg agcagcgtct ccaagtagaa actgttgcgg tcgctcaact
      481 cgtggtcgta atgcacgaat gcgacgccgc gatccgccgg cgtgtcgact tcatagtccc
      541 ggtcgatgcc gtattcgaga gtcggccgcg cttggcaatt gcagccgccc gacaagagac
      601 cgacgccgct gaacggcaac ggacggaaat tgccgctgcc gtcgggtaag aactcgatgt
      661 gatcgaccgc cgaacccggc tgcagcagca ggccgccctc ggtgaaattc gtcgggctca
      721 cgaacggccg cgccaggaaa tcgggcccgt tcacgggatc cggattcgtg acctgggctt
      781 tgagatccaa gtaatcgctg cgagcgagca gcgattcgaa gtcgcggatt ccctcttttt
      841 cccaccattc gcccgacacg atgatgtgac ctttttcgcc gacgtcgccg ccccacgcga
      901 ggcccgcttt gtagctctcg ccgtcggact ccgtcgtctg gcccgcttgg aactgatact
      961 tgatgcccgt gtagttgcgg tcgagacgaa agttcacgac gcctgcgacg gcatcggcgc
     1021 cgtaggccgc cgatgcgcca ccggtgacgg cttcgacgct cgacacgagc atctccggaa
     1081 tcacgctgac gtcgacggtg ccgaatttgt tgcttggccc aaggcgatgc ccgtccatca
     1141 gcacgagcgt tcgccgcgat tccaggcccc gcaagttcaa gttgctgccg ccggacgcaa
     1201 ccgagccgcc gaccgcctgc tggctcgtga ggtcgttata gaacacaggc aactggctca
     1261 aggattcgat cagcgtcgtc ggcgccatcg tttgcagctc ggcggcgctg acggccgtca
     1321 cgggcgtggg cgtgttgact cccgactgga tgagccgtga gccggtgacg gtcacctcct
     1381 cgaccggttc ttgcgcgaga gcctgctgtc ccgccattcc aagacaagca acgtagaccg
     1441 ccttggcaag cccgttgtaa ccgagaccca cgatcttttt cccgttcatg gtttcaaccc
     1501 cccgaggttt taggccgcag aaaaaaagaa agcgaaagcg ttgcccgacc gcgttgcgcc
     1561 ggtagcaggg cgcatcgtga gtaggcctgg gcgcgtcgct tagttcatac atatagtagt
     1621 agcgcacgct gtaagcggta aacggctttt atcggagcgg ggggcagcct gtagctccgt
     1681 cacaacatgg gagtgggttt cgcggcgggt tgcgatcgac tcgttggcat ggcgcgtgcc
     1741 gcgaacgcga tgcgcgtcga gcggcttcgg ttcttcggcg gcggagcttc gatcggtttt
     1801 gcgcgtcgct gctggccggt ttgaaccttc ggctgcggtg ccgggacgcg gcgtggatct
     1861 cgtggtgcgc gcgcaaccgt cgtcgccatt tgacacgcgt tgccgcgcaa cctaacgtct
     1921 ccttcatgac acacgtcgcg tgtcacacac ctgttcacca agtcacagcg ctggctcagg
     1981 acgcgggctc cgcgttctgc gcatgcctat cggtttgagg ggggaggggc cccgt
//
LOCUS       DASSNN010000060         4888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1267593, whole genome shotgun sequence.
ACCESSION   DASSNN010000060 DASSNN010000000
VERSION     DASSNN010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267593"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1538
                     /locus_tag="VFL84_01050"
     CDS             135..1538
                     /locus_tag="VFL84_01050"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HET7607236.1"
                     /translation="MQPIGKVKPPAERAFELLKDVPLGVFLVDSELRIRGANEAARQS
                     FSNAPVGHELREVADVLHARGGVICYLGGATDTEPERRLAELLRSGECGPPSSSRAKD
                     DFLATVSHELRSPLQGILGWVTLLQGGRLDAAQTTRALQSVERSVRLQAQLVNDIMDI
                     ARIESGKVDLDETAVDLAQLLTTTAEEFLPQARARRIDLRADMKPCGIVSGDKERLHQ
                     VFANLLSNALKFTPTGGSVSIACHREGGDVVTTVTDSGDGIEPAFIPHLFKRFSQADT
                     SITRRHGGLGLGLAIVRHLVELHGGTVEAKSDGRDRGATFTVRLRAANDDGARRPGAD
                     TESREPGRLDGLEILLVENDQDTLEAMTHGLTALGASVRAATSVDEAWRSFVHRAPHL
                     VLTDLSMPHEDGYSLLRRIHSQQNRPPVVALTGLTRPEDKEQVERAGFAAFVAKPIDL
                     RQLVAILNDLYPKGAHS"
     gene            complement(1529..4198)
                     /locus_tag="VFL84_01055"
     CDS             complement(1529..4198)
                     /locus_tag="VFL84_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HET7607237.1"
                     /translation="MALQAEIASDVRYALRGFKRTPGLTIITVLTLAIGLGATAAIFS
                     VVNAVLLKPLPYADPERLVQIVENVPAAEGFGGAAQRRTGINASELDRWRTGLKTISQ
                     IAVMQREGRTFATADGTVQIYGQRVSPALFSMRAVQPLLGRGLLPDDERPDTDVVVIS
                     EATWREYFNSAPDIVGQRMQLDTTTYTVVGVMPPAFGEEAFWTAFFVPPMQPGQFFIL
                     PAQAQLAPGVSLEAASAEANTVGLQLRASAPAVPPGPGARPAPGGGPAPGGGPAPGAR
                     PPGAAVGPAPGAAPAAGQPAGSSVVPRPRFEVVRTQDELTAAVAPALRVLVVAVGVVL
                     AIVCTNVANLLLVRGARRQQEIAIRRSLGATRGRIIRQVLTESLVLAGFAAITAIVFA
                     FAGVSFLKTLSTSYVNPRFAFAATVLPRLDEIAIDPAVLAFVVVLSVVTAMLFGLLPA
                     LRLSKYGEHGHASESQLSAVTRNSSLGRVLATVQLACAMALLIGAGLLVNSFMKLTGI
                     DPGFDPSGVLSFELIVPPDSTAERKLEIAETFVARLESDARVTGAGFSDIPPLTAGIM
                     IFMGMWIPEGKTQAEMAEERRAQPPEETTQTRQVSSGYLRGLGARLVAGNWLDDRAET
                     GPAVLVSRPYADQYFPNGNAVGATLTAGGPTGTSAVTIAGVVDDIHLGRLEGKAERVI
                     FLDPRYNLARQPTPRPPQADRAFLTIGGGGIAFAARTTGDPAAIIRDLRGIARDIDPR
                     LAVDAAVPMERIVSSLTTRPRFYAALLSTFGAIAGFIAVIGLYGVLSYVVGQRTKELG
                     IRMALGAQRAAVLKLVLRQGAIIVAVGVAAGVAGAAALTRYLAAMLYDLGALDVTTFT
                     VVAVVFAAAAMAAAYVPARRATAIDPLAALRYE"
     gene            complement(4296..>4888)
                     /locus_tag="VFL84_01060"
     CDS             complement(4296..>4888)
                     /locus_tag="VFL84_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7607238.1"
                     /translation="PSPVGLSAIYIPVPQAPVWPFTQIIVKRLGEESRARSTIFEALE
                     RVDPTIAPDVMVYDDMLERMTFFASTMTKLFGACGAFAILLAVTGIYGMSSNSVLVRS
                     HEIGLRRALGASNNAVVATFVSQGVKQLARGLAVSALLCAGVLVVLQLGFSIGYWAMG
                     LLAGVVLVVSGCVLLSIYLAVRRVIRLEPSAALRAG"
BASE COUNT          847 a         1662 c         1647 g          732 t
ORIGIN      
        1 atctcttcgg acagcttcag ctcggtcagt accgggggtg cggcggcgct catcgaatga
       61 ctccttccat cagggcgagt cggatactag cgcatcccga gcggttggca cgcttctggc
      121 agcttcggcg gagcatgcag cccatcggga aagtcaaacc gcccgccgag cgggcgttcg
      181 agctcttgaa ggacgtgccg ctcggcgtgt ttctcgtcga cagcgagctc cgaattcgcg
      241 gcgcgaacga ggccgcgcgc cagagcttct cgaacgcgcc ggtcgggcac gagctgcgcg
      301 aggtggccga cgtgttgcac gcccgcggcg gcgtgatctg ctatctcggc ggcgcgaccg
      361 atacggagcc ggagcggcgc ctggccgagc tgctgcggtc gggagaatgc gggccccctt
      421 cgtcgagccg cgcgaaagac gacttcttgg cgacggtttc gcacgagctg cgctcgccgc
      481 tacaaggcat cctcggctgg gtgacgctgc tgcagggcgg caggctcgac gcggcacaga
      541 cgacgcgcgc gctgcaatcc gtcgagcgca gcgtgcggct ccaagctcag ctcgtgaacg
      601 acatcatgga catcgcgcgc atcgagtcgg gcaaggtcga cctcgacgag accgccgtcg
      661 acctcgccca gctgctcacg actaccgccg aggagtttct gccgcaggcg cgagcgcgcc
      721 ggatcgacct gcgcgccgac atgaaaccgt gcggcatcgt gagcggcgac aaggagcgcc
      781 tgcatcaagt ctttgcgaac ctactgtcga acgcgctgaa gttcacgccg accggcggct
      841 ccgtgtcgat cgcctgccat cgcgaaggag gcgacgtcgt gacgaccgtc accgatagcg
      901 gcgacggcat cgaacccgcg ttcatcccgc atctgttcaa gcgcttctcg caagccgata
      961 cgtcgatcac gaggcggcac ggcggcctcg gcctcgggct cgcgatcgtc aggcacttgg
     1021 tggagctgca cgggggcacg gtcgaggcca aaagcgatgg gcgcgaccgc ggcgccacgt
     1081 tcaccgttcg gctacgcgcg gcgaacgacg atggcgcgcg ccgcccgggc gcggataccg
     1141 agagccgcga gccgggcagg ctcgacgggc tcgagatcct gctcgtcgaa aacgatcagg
     1201 acacgctcga agcgatgact cacgggctca cggcgctcgg cgcgagcgtg cgcgccgcga
     1261 cctcggtcga cgaggcgtgg cgctctttcg tgcatcgagc gccgcatctg gtcctgacgg
     1321 atttgagcat gcctcacgag gacggctact cgttgctgcg cagaattcat tcgcagcaga
     1381 accgcccgcc ggtcgtcgcg ctgacggggc tcactcgacc cgaggacaaa gagcaagtcg
     1441 agcgcgccgg ctttgccgcg ttcgtcgcga agccgatcga tttgcggcag ctcgttgcga
     1501 tcttgaacga cctgtacccg aagggcgctc actcgtaacg caatgccgcg agcggatcga
     1561 tcgccgtcgc tcggcgcgcc ggcacgtacg cggcggccat cgcggccgcc gcgaacacga
     1621 cggcgacgac ggtaaatgtc gtcacgtcga gcgcgccgag gtcgtacagc atcgcggcga
     1681 gataacgcgt cagtgcagcg gcgccggcca cgccggccgc gactccgacg gcaacgatga
     1741 ttgctccttg ccgcagcacg agcttcaaca ccgcggcccg ctgcgcgccg agggccatgc
     1801 gaatgccgag ctctttcgtg cgctgcccga cgacgtacga cagcacgccg tagaggccga
     1861 tcacggcgat gaagccggcg atggcgccga acgtgctcaa tagcgcagcg tagaagcggg
     1921 gtcgcgtcgt aaggctcgag acgatgcgct ccatcggtac ggcggcgtca acggcgagcc
     1981 gcggatcgat gtcgcgcgcg ataccgcgca ggtcacgaat gattgccgct gggtcgcccg
     2041 tcgttcgcgc cgcgaacgcg atgccgccgc cgccgatcgt caggaacgcc cggtcagcct
     2101 gcggcgggcg cggcgtcggc tgccgcgcca agttgtaacg cgggtcgaga aagatcacgc
     2161 gctccgcctt gccttcgagg cggccgagat gaatgtcgtc gacgacgccg gcgatcgtca
     2221 cggcgctcgt tccggtgggt ccgccggccg tcagcgtggc gccgacggca ttgccgttcg
     2281 ggaagtattg gtcggcatac gggcgactca cgagcacggc agggccggtc tccgcccggt
     2341 cgtcgagcca attccctgca acgaggcgcg cacccaatcc gcgcagatag ccggagctta
     2401 cttggcgcgt ctgtgtggtc tcttcgggag gttgcgcccg ccgctcttcc gccatctcgg
     2461 cctgcgtctt accttccggg atccacatgc ccatgaagat catgatgccc gcggtcagcg
     2521 gcgggatgtc cgagaagccc gcgcccgtca cgcgtgcatc gctttcgagg cgagctacga
     2581 acgtctccgc aatctcgagc ttgcgttccg ccgtggagtc tggaggcacg atgagctcga
     2641 agctcaagac gccgctcgga tcgaagcccg ggtcgatgcc ggtgagcttc atgaaactgt
     2701 tcacgagcag gccggcgccg atcagcagcg ccatcgcaca cgccaactgc accgtcgcaa
     2761 gcacgcgacc caaactcgag ttgcgcgtaa ccgcagagag ttgcgactcg gaggcgtgac
     2821 cgtgctcgcc gtacttcgac aggcgcagcg ccggcagcag accgaacagc atagcggtca
     2881 cgaccgacag cacgacgacg aacgcgagca cggccggatc aatcgctatc tcgtcgagtc
     2941 gcggcagcac cgtagcggca aaagcgaagc gcgggttgac gtacgacgtc gatagggtct
     3001 tgaggaagct cacgcccgcg aatgcgaaca cgatcgcggt tatcgcagcg aaccctgcga
     3061 gcacgaggct ttcggtaagc acctgccgta tgatccggcc gcgcgtcgcc ccgagtgaac
     3121 gccggatcgc gatttcttgt tgacgccgag cgccgcgcac gagcagcaag ttcgcgacgt
     3181 tcgtgcacac aattgcgagc acgacgccga cggcgacgac gagcacgcgc aacgccggcg
     3241 ccacggctgc cgtgagctcg tcctgcgtgc gcacgacctc gaaccgcggc cgcggtacca
     3301 cgctgctacc tgctggctgc cctgccgccg gcgccgcgcc gggcgccggc ccaacggcag
     3361 cgccgggcgg ccgagcgcct ggcgccggcc caccgccggg tgccggccca ccgccgggtg
     3421 ccggccgggc acctggccca gggggcaccg ctggcgcgct ggcgcgcagc tgcaacccta
     3481 ccgtgttcgc ctcggcgctc gccgcctcga gcgaaacgcc cggcgcaagc tgcgcctgcg
     3541 ccggcaggat gaagaattgg ccgggctgca tcggcggcac gaagaacgcc gtccaaaacg
     3601 cctcctcgcc aaatgccggc ggcatgacgc cgacgaccgt gtaagtcgtg gtatcgagct
     3661 gcatcctttg cccgacgatg tctggcgcgg agttgaagta ctcccgccac gtcgcttcgc
     3721 tgatcacgac gacgtcggtg tccggccgct cgtcgtcggg caacaagccg cggccgagca
     3781 gaggctgcac cgcgcgcatg ctgaacagcg cgggcgaaac gcgctggccg tagatctgca
     3841 cggtgccgtc cgcggtcgcg aacgtccgcc cttctcgctg catgacggcg atctgcgaga
     3901 tcgtcttcaa gcccgtccgc catcggtcga gctcggatgc gttgatcccg gtcctgcgct
     3961 gtgcggcgcc gccgaagccc tccgcggccg gcacgttctc gacgatttgc acgagccgtt
     4021 ccggatccgc atagggcagc ggcttgagca ggacggcatt cacgacgctg aagatcgccg
     4081 ccgtcgcgcc gagcccgatg gcgagcgtca gcaccgtgat gatggtgaga ccgggtgtgc
     4141 gcttgaagcc gcgcaatgcg taacgaacgt cggaagcgat ttcggcttgc aatgccatga
     4201 cgctagtccc ccccgggtca gcgctcgctt gagcgtgacg gatcgtaaca ccagtactta
     4261 ttctcgccag agctcgaaaa gttccctgcg tcgagctaac cggcgcgcag cgcggcgctc
     4321 ggctccaacc gaatcacgcg gcgcacggcg agatagatcg agagcagcac gcagccggaa
     4381 acgacgagca cgactccggc gagcagcccc atcgcccagt agccgatcga aaagcccagt
     4441 tgaagaacga cgaggactcc ggcgcagagc agcgcgctga cggcgagccc gcgagcgagt
     4501 tgcttcacgc cctgggacac gaacgtcgcg acgacggcgt tgttgctcgc gccgagcgcc
     4561 ctgcgcaacc cgatctcgtg gctgcgcacg aggacggaat tgctgctcat gccgtagatg
     4621 ccggtgactg cgagcaggat cgcgaacgca ccgcaggcgc cgaacagctt cgtcatcgtg
     4681 ctcgcgaaaa acgtcatgcg ctcgagcatg tcgtcataga ccatgacgtc cggcgcgatc
     4741 gttggatcga ctcgctcgag cgcctcgaat atcgtgctgc gtgcgcggct ttcctcgccg
     4801 agccgcttga cgatgatctg agtgaacggc cacaccggcg cttgcggcac cgggatgtag
     4861 attgccgaga ggccgacggg agacggcg
//
LOCUS       DASSNN010000061         7079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_260055, whole genome shotgun sequence.
ACCESSION   DASSNN010000061 DASSNN010000000
VERSION     DASSNN010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260055"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VFL84_01065"
     CDS             <1..647
                     /locus_tag="VFL84_01065"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HET7607239.1"
                     /translation="LIKILRLPLRSFLPALTLGNTGNLGLPLCLFAFGEEGLGLAVAV
                     YVTNSLLQFTFVPLLHSRVSVVRTLFSTPMLYGAAAGLAVLIAEVPVPVWLANTLGTL
                     GDLLIPLMLMALGNTIGGLKAHNLPRALGLGGARLAISFTVAVAVSFSLGLRGVAQGV
                     LVLQGAMPAAVFSYLFAAHYERDADDIAGIVLVSTLLGALALPFLVSYALWLAR"
     gene            782..1618
                     /locus_tag="VFL84_01070"
     CDS             782..1618
                     /locus_tag="VFL84_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018948164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET7607240.1"
                     /translation="MSTTIRLSMAALLAVAAAACATSPTGRRQLALVSEESAIVASAE
                     AYSMLIGELRKEGKIVPESSRVARRVEGITERLVAQAVEKRPETADWDWSIEVIDDPE
                     VVNAWCMAGGRMAVYTGLIQKLDPTDDELAQVLAHEISHALANHTAERMSVALATNVG
                     ILLAGVLSEDSQKTMSRAAIAAALAVQLPNSRSAESEADEIGIELAAKAGYNPNAAIS
                     LWEKMANVSGSGPPQFLSTHPSPANRQERLAELAPQMMAYYEEARRNAPTRSVARVRR
                     VD"
     gene            complement(1642..2925)
                     /locus_tag="VFL84_01075"
     CDS             complement(1642..2925)
                     /locus_tag="VFL84_01075"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET7607241.1"
                     /translation="MLGVDARRIAVIGGIRIPFCRAHTAYARCSNQDMMTAVLQALVA
                     RYSLSGVRLGDVSLGAVIKHSRDFNLARESTLSSGLAPETPAFDIQRACGTSLSAAII
                     IGTKISTGLIEAGIAGGTDSISDVPLVYPDAYRALLLESARSRSFGGRLSPWLKLRPK
                     HFKPIFPDVMEPRTGLSMGESMEVTAKQWHLSREEQDKLALASHQNAARAYAEGFYDD
                     LVVEFKGVKRDNNIRTDTSLEQLAKLKPVFDRGPGGTLTAGNSTPLTDGASAVLLSSE
                     DWARARGLPISAYLTHARETAVDFIAKEGLLMAPAYAVSEMLAEANLALQDFDFYEIH
                     EAFAAQTLVTLKAWESEQFCRERLGRDRAMGSIDRAKLNIKGGSVAVGHPFAATGTRI
                     LATLAKLLRERGRGRGLISVCTAGGMGVTAILEAA"
     gene            3045..3479
                     /locus_tag="VFL84_01080"
     CDS             3045..3479
                     /locus_tag="VFL84_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607242.1"
                     /translation="MGFASALAVFATGCAASDPEAEIRALIGAAEQAAEARDVGFFSA
                     VLAESFRDGRGDDRDATIRALRGYFIANQRIEILSRVDEIVIEGGTAARVVVHAGMVG
                     RRAGAAVIDGIDADLYRFELELVDDGGEWKVIGADFGRAVGE"
     gene            3481..3954
                     /locus_tag="VFL84_01085"
     CDS             3481..3954
                     /locus_tag="VFL84_01085"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HET7607243.1"
                     /translation="MRYVAAFFAAVVLGSAPASSASAQPQVAGLRVALQRLEPLEPTG
                     RGQRFRAALLVDNMNTEPFKIRNIEFKLNLADQGIVDGNTGAFTIEALDQQTLTLELS
                     SEIVSSVSRLLSFVQGPDNKLPYEIFGKVTLDRRNMQTLQFSAHGLVPLVMPGPR"
     gene            3968..6013
                     /locus_tag="VFL84_01090"
     CDS             3968..6013
                     /locus_tag="VFL84_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7607244.1"
                     /translation="MSLRLQLLAVALLTLVLPWTGFWYVQEMEAALRASLEQSLLARA
                     ATVATALVEQNTALCVPPDCDPLRAGATVYATELAREPELDGVPEDNWNVAPETGMAI
                     GADHRVWAGTYGRFVYLYVTVADGDLVYQKQAGQPPYGDRFVIASEPGTPRWWLLATA
                     APGTFRAQETGPDRFEPSEVYDGRILGAWLETPSGYALEVRVPLNLVGGALGVGVIDV
                     DRSGATHTVRLDATWDEATGTLGRFVRQVPELTTSLAQFGGAGGRFRVLDKDGWVLAT
                     TGRVAPRARADDGAGLPGDFLRWLLEREDPPYPPERPTGRVADATLRQALDGHEATAW
                     YGSGPDREAIVAAAVPILGAGGVEGAVLLEQASDPILTVTNRALVRLMSLTLLVTLVV
                     GIGLLGYATWLSLRVRRLAGAAQTALGPRGEIRSGMPGAAAGDELGALARSFAELLER
                     LREHTEYLRTLASKLSHELRTPLAVVTTSLDNLEHEIAGAQTDEYLKRLRQGTERLDR
                     ILAAMSEATELEQAIRETPAQPFDLAAVVASCCAAYRDVYPEREIEYRSEAGLATIVG
                     SDELIAQLLDKLVDNAVSFSAPGSRVDIVLGAAADELVLSVANRGPRLPEKMRGRLFD
                     SLVSIREYRDGRPHLGLGLHIAALVADFHGGRCEADDLPDGSGVIFKVWFPRANGAR"
     gene            complement(6164..>7079)
                     /locus_tag="VFL84_01095"
     CDS             complement(6164..>7079)
                     /locus_tag="VFL84_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7607245.1"
                     /translation="LDKGVVDRRVELLKASGIEFVTGADVGKNVDPKKLRAEHDALLL
                     TTGATLPRDLKIPGRGLGGIHFAMEFLTANTKSLLASNLEDGNYISAKDKRVIVIGGG
                     DTGADCIGTSLRHGCKSLINLELLDQSPAKRALDNPWPLWPKIHRSDYAHEEAIARFG
                     ADPRNYAVVSKEFLGSDGRVTGVRINRVQWVDVNGAMKMMDVEGSAETLEADLVLLAM
                     GFLGPESYLADALGLERDQRSNYRAAHGQFATSSPGVFAAGDCRRGQSLVVWAINEGR
                     GAARAIDAFLMGESQLPAPKLTLGGVTR"
BASE COUNT         1089 a         2360 c         2470 g         1160 t
ORIGIN      
        1 cgctgatcaa gatcctcagg ttgccgctcc gttcgttctt gcccgcgttg acgctcggca
       61 ataccggcaa cctcggattg ccgctctgcc tgttcgcgtt cggcgaggaa gggctcggcc
      121 tcgccgtagc cgtctacgtg acgaactcgt tgctgcagtt cacgttcgtg ccgctgctgc
      181 acagccgcgt gtcggtcgtg cgcacgctgt tctcgacgcc gatgctgtac ggcgccgccg
      241 ccggtctcgc cgtcttgatc gccgaggtcc ctgtgccggt ctggctcgcg aacacgctcg
      301 gcacgctcgg cgacttgctg atcccgctca tgctcatggc tcttggcaac acgatcggcg
      361 ggttgaaggc acacaacttg ccgcgcgcgc tcggcctggg cggcgcccgg ctcgcaattt
      421 cgttcacggt cgccgtcgcc gtcagcttct cgctgggctt acgcggcgtc gcgcaaggcg
      481 tgctcgtgct gcagggcgcg atgccggcgg cggtcttcag ctatctgttc gcggcgcatt
      541 acgagcgcga cgccgacgac atcgcgggca tcgtgctcgt ctcgacgctg ctcggcgcgc
      601 tggcgttgcc gttcctcgtg tcgtatgcgc tgtggctcgc gcgctgacgg cgtccggccg
      661 aagtcgagtg gcgcgggcga gctgagctag ccgataccaa gccgcccgcg gcgccgcgct
      721 gtctcgcgct ggcatcggtt ttgcacctcc gcggagacaa atcgagccgg aggttcagcc
      781 gatgagcacg accattcgtc tttcgatggc ggccttgctg gccgtcgccg cggccgcctg
      841 tgcgacgagc ccgacgggtc gccgccagct cgcgctagtg tccgaggaga gcgcaatcgt
      901 cgcgtcggcg gaagcctact cgatgctgat cggcgagctg cgcaaggagg gcaagatcgt
      961 gcccgagtcg tcgcgcgtgg cgcggcgagt cgaggggatc acggagcgcc tcgtcgcgca
     1021 ggcggtcgag aagcggcccg agaccgcgga ttgggactgg agcatcgagg tcatcgacga
     1081 ccccgaggtc gtcaacgcct ggtgcatggc gggcggccgc atggccgtgt acacgggcct
     1141 catacagaag ctcgacccca ccgacgacga gctcgcgcaa gtcctcgcgc acgagatcag
     1201 ccacgcgctc gcgaatcaca cggcggagcg catgtcggtc gcgctcgcga cgaacgtcgg
     1261 catcctgctg gccggcgtgc tgtccgagga ctcgcagaag acgatgagcc gggcggccat
     1321 cgccgccgcg ctcgcggtgc agctgccgaa cagccgttcc gccgaatcgg aggccgacga
     1381 gatcggcatc gagctcgcgg ccaaggcagg ctacaacccg aatgcggcca tcagcctgtg
     1441 ggagaagatg gcgaacgtgt cgggcagcgg gccgccgcaa ttcttgagca cgcacccgag
     1501 ccccgcgaac cggcaagaaa ggctcgccga gctcgcgccg cagatgatgg cctactacga
     1561 ggaggcgcgc cgcaatgcgc cgacgcgctc cgtcgcgcgc gtccggcgag tggactgacc
     1621 cagcagctcg ccggcagggc gtcaggccgc ttcgaggatc gccgtgacgc ccatgccgcc
     1681 ggccgtgcaa acggagatca agccgcggcc gcgcccgcgc tcgcgcagca gcttcgcgag
     1741 cgtcgcgagg attcgcgtgc cggtcgccgc gaacggatgg ccgacggcga cgctgccgcc
     1801 tttgatgttg agcttggcgc ggtcgatcga gcccatcgcg cgatcgcggc cgagccgctc
     1861 gcggcagaac tgctccgatt cccacgcttt cagcgtcacg agcgtctgcg ccgcgaacgc
     1921 ttcgtggatc tcgtagaaat cgaaatcctg cagcgccaaa ttggcttccg cgagcatctc
     1981 cgagacggcg taggccggag ccatcagcaa gccttccttg gcgatgaagt cgacggccgt
     2041 ttcacgcgcg tgggtcaggt atgccgagat cggcaggccg cgcgcgcgag cccagtcctc
     2101 ggaggagagc agcacggccg acgcgccgtc cgtcagcggc gtgctgttgc cggctgtcag
     2161 cgtgccaccg gggccgcggt cgaaaaccgg cttgagcttc gcgagctgct cgaggctcgt
     2221 gtcggtgcga atgttgttgt cgcgcttgac gcctttgaac tcgacgacga ggtcgtcata
     2281 gaagccttcg gcgtacgcgc gcgccgcatt ctggtggctc gcgagcgcga gcttgtcttg
     2341 ctcctcgcgc gacaagtgcc actgcttcgc ggtgacttcc atgctctcgc ccatcgacag
     2401 tcccgttcgc ggctccatga cgtcggggaa aatgggcttg aaatgcttcg gccgcagctt
     2461 gagccatggc gagaggcggc cgccgaacga gcggctgcgt gcgctctcga gcagcagcgc
     2521 gcgataggcg tcggggtaga cgagcggcac gtcgctgatc gagtccgtgc cgcccgcaat
     2581 gccggcctcg atgaggcccg tcgaaatctt ggttccgatg atgatcgccg ccgacaggct
     2641 cgtgccgcag gcgcgttgga tgtcgaacgc cggagtctcg ggcgcgaggc ccgacgacag
     2701 cgtcgactcg cgcgcgagat tgaagtcgcg cgagtgcttg atgacggcgc cgagcgaaac
     2761 gtcgcccagg cgcacgccgg agagcgaata acgcgcgacg agcgcctgca gcacggccgt
     2821 catcatgtcc tggttcgagc agcgggcgta agccgtgtgg gcgcggcaga acgggatgcg
     2881 aatgccgccg atgacggcga tccgacgagc atcgacaccg agcataagac gctccgctca
     2941 aacggtcctt gtgccaagta taaaggtcca acagttgcgc cgggtgcggt acggcgtcgt
     3001 gtttttgcgc gtgcaggcac tctacaatcg ccgccatgaa agccttgggg tttgcttcgg
     3061 cgctcgccgt tttcgcgacc ggctgcgcag ccagcgaccc ggaagccgag atccgagctc
     3121 tgatcggcgc ggccgagcag gcggccgagg cgcgcgacgt cggtttcttc agcgcggttc
     3181 tggccgaaag tttccgcgat gggcgcggcg acgatcgcga cgcgacgatc cgcgcgctgc
     3241 gcggctactt catcgccaac cagcgcatcg agattttgag ccgcgtcgac gagatcgtca
     3301 tcgagggcgg cacggccgcg cgcgtggtcg tgcatgccgg catggtcggc cggcgcgcgg
     3361 gcgccgcggt catcgacgga atcgacgccg acctctatcg cttcgagctc gagctcgtcg
     3421 acgatggcgg cgagtggaaa gtcataggcg ccgacttcgg gcgcgcggtg ggagagtgag
     3481 atgcgatacg tagcggcatt cttcgcggcg gtcgtgctcg gcagcgcgcc ggcaagctcg
     3541 gcgtcggcgc agcctcaagt ggcgggactc cgagtcgcgt tgcagaggct cgagccgctc
     3601 gagcccaccg gtcgaggcca gaggttccgc gccgcgctgc tcgtcgacaa catgaatacg
     3661 gagccgttca agatccgcaa catcgagttc aagctgaatc tcgccgatca gggcatcgtc
     3721 gacggcaaca cgggcgcgtt cacgatagag gcgctcgatc agcagacgct cacgctcgag
     3781 ctcagcagcg aaatcgtctc gtcggtttcg cgcctcttgt cgttcgtgca aggtcccgac
     3841 aacaagttgc cctacgagat cttcggtaag gtcacgctcg acaggcgcaa catgcagacg
     3901 ctgcaattca gcgcgcacgg cttggtgccg ctcgtcatgc ccgggccgcg ctgacagctc
     3961 acgcgccatg tcgctgcggc tgcagttgct cgccgtcgcc ctgctcacgc tcgtccttcc
     4021 atggacgggc ttttggtacg tgcaggagat ggaagccgcg ctgcgagcga gcctcgagca
     4081 atcgttgctg gcgcgagcgg cgaccgtcgc gaccgcgctc gtcgagcaaa acaccgcgct
     4141 ctgcgtgccg cccgattgcg atccgctgcg cgccggcgcc accgtctacg cgaccgagct
     4201 cgcgcgtgag cccgagctcg acggtgtccc ggaggataat tggaacgtcg cgcccgaaac
     4261 cggtatggca atcggcgccg accaccgggt ctgggccgga acgtacgggc ggttcgtcta
     4321 tctctacgtc accgtcgcgg atggcgatct cgtctatcag aagcaggcgg ggcagccgcc
     4381 gtacggcgat cgtttcgtga tcgcctccga gcccggcacg ccgcgttggt ggctgctcgc
     4441 gacggccgcg ccgggcacgt ttcgcgccca ggaaacgggc cccgaccggt tcgagccgag
     4501 cgaggtctac gacggccgga ttctcggagc gtggctggag acaccgtccg gctatgcgct
     4561 cgaggtgcgc gttccactga acctcgtcgg cggggcgctc ggcgtcggcg tcatcgacgt
     4621 cgaccgcagc ggcgccactc atacggtccg gctggacgcg acctgggacg aggcgaccgg
     4681 cacgctcggg cggttcgtgc gccaggtgcc cgagctcacg acgtcgctcg cccagttcgg
     4741 cggagccggc ggccgtttca gagtgctcga caaagacggc tgggtgctcg cgacgacggg
     4801 ccgcgtagcg ccgcgcgcga gggccgacga cggcgccggg ctcccgggcg atttcttgcg
     4861 ttggctgctc gagcgcgagg acccgcctta tccgcccgag cgtccgaccg gccgcgtcgc
     4921 cgacgcgaca ttgcggcaag cgctcgacgg ccacgaggcg acggcatggt acggcagcgg
     4981 accggatcgc gaggcgatcg ttgccgctgc cgtaccgatt ctaggcgccg gcggcgtcga
     5041 aggcgcagtg ctgctcgagc aggcgagcga tccgatcttg acggtgacga accgcgcgct
     5101 cgtgcggctc atgtcgctga cgttgctcgt gacgctcgtc gtcggcatcg gcctgctcgg
     5161 ctacgcgacg tggctgtcct tgcgcgtgcg ccgcctcgcc ggcgcggccc aaacggcgct
     5221 tgggccgcgc ggcgagattc gcagcggaat gccgggcgcc gcagccggcg acgagctcgg
     5281 agcgctcgcg cgcagcttcg ccgagctgct cgagcgcctg cgcgagcaca cggagtacct
     5341 gcgcacgctc gcgagcaagc tctcgcacga gctgcgcacg ccgctcgcgg tcgtcacgac
     5401 gtcgctcgac aatctcgagc acgagattgc gggagcgcag accgatgaat atctgaagcg
     5461 tttgcgtcag ggaacggaac ggctcgaccg gattctcgcg gcgatgagcg aggcgacgga
     5521 gctcgagcag gcgatccgcg agactccggc gcagccgttc gacctcgcgg cagtcgtcgc
     5581 gtcgtgctgc gccgcgtatc gcgacgtgta tccggagcgc gagatcgagt atcgcagcga
     5641 ggcgggactg gccacgatcg tgggctccga cgagctcatc gcgcagctgc tcgacaagct
     5701 cgtcgacaat gcggtgagct tcagcgcgcc gggcagccgc gtcgacatcg tgctcggcgc
     5761 ggccgcagac gagctcgtgc tctccgtcgc gaaccgcgga cctcgcttgc ctgagaagat
     5821 gcgcggacgg ctgttcgact cgctcgtctc gatccgcgag taccgcgacg gccggccgca
     5881 cctggggtta gggcttcaca tcgccgcgct cgtcgccgac ttccacggcg gccgctgcga
     5941 ggccgacgat ctgcccgacg gcagcggcgt catcttcaaa gtctggttcc cgcgggcaaa
     6001 cggcgcgcgt taggtaggga tttctcgctg tgtgcgaaga ggccctcccg agagcgtgtg
     6061 agcacatgga tgtgcgaaca cgagccgcag ggacgctctt ccggcgtctc tcgggagggc
     6121 ctcttcgcgc gcagcgagaa atcagcggtg atgcaaaacg aagctaacgc gtgacgccgc
     6181 cgagcgtgag cttcggcgcc gggagctggc tttcgcccat caggaacgcg tcgatggcgc
     6241 gcgcggcgcc gcggccttcg ttgatggccc agacgacgag cgactggccg cggcggcagt
     6301 cgccggccgc gaacacgccg ggcgagctcg tcgcgaattg cccgtgcgcg gcgcgataat
     6361 tcgagcgctg atcgcgctcg aggccgagcg cgtcggcgag ataggactcc ggccccaaga
     6421 agcccatcgc gagtagcacg aggtcggcct cgagagtttc ggcggagccc tcgacatcca
     6481 tcatcttcat cgcgccgttg acgtcgaccc attgcacgcg gttgatgcgc actcccgtga
     6541 cgcgtccgtc ggagcccaag aactccttcg agacgaccgc gtagttgcgc gggtcggcgc
     6601 cgaagcgcgc gatggcttcc tcgtgcgcgt aatcggagcg atggattttc ggccacagcg
     6661 gccacggatt gtcgagcgcg cgcttcgccg gcgactggtc gagcagctcg aggttgatca
     6721 agctcttgca gccgtgacgc agggacgtgc cgatgcagtc ggcgcccgta tcgccgccgc
     6781 cgatgacgat gacgcgtttg tccttcgcgc taatgtagtt gccgtcctcg aggttgctcg
     6841 cgagcaagct cttcgtgttg gccgttagga actccatcgc gaaatggatg ccgccgagcc
     6901 cgcggccggg gatcttgaga tcgcgcggca gcgtcgcgcc cgtcgtcagc agcagcgcat
     6961 cgtgctcggc gcgcagcttc ttcggatcga cgtttttgcc gacgtcagcg cccgtgacga
     7021 actcgatgcc ggatgctttg agcagctcga cgcggcggtc gacgacgccc ttgtcgagc
//
LOCUS       DASSNN010000062         5335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_518915, whole genome shotgun sequence.
ACCESSION   DASSNN010000062 DASSNN010000000
VERSION     DASSNN010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518915"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /gene="ftsZ"
                     /locus_tag="VFL84_01100"
     CDS             <1..861
                     /gene="ftsZ"
                     /locus_tag="VFL84_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET7607246.1"
                     /translation="FITAGLGGGTGTGAAPVVAQLAKEMGILTVAVVTKPFGMEGPKR
                     MRVAEQGIAELGRFVDSLITIPNEKLLSVLGPSTTLLDAFRSANEVLQGAVQGIAELI
                     TRPGLINVDFADVRTVMSEMGMAMMGSGVASGQDRARTAAEAAVSSPLLEDINLSGAN
                     GVLVNVTAGVDLSIGEFQEVGDTVKQFASDDATVVIGTVIDPEMTDEIRVTVVATGLG
                     QAIGMAERPMKVVRRAAPPAPKGREPNYAELERRPAVERKRAVGDGVTGNDSLEDLLD
                     IPAFLRRQAD"
     gene            1019..1927
                     /gene="lpxC"
                     /locus_tag="VFL84_01105"
     CDS             1019..1927
                     /gene="lpxC"
                     /locus_tag="VFL84_01105"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HET7607247.1"
                     /translation="MRQRTLKEAIRASGIGLHSGEKVYMTLRPAPANTGIVFRRLDLP
                     EPVDVPAEATRVTETTLGTTLERESARIGTVEHLMSAMAGLGIDNAFVDLTAAEVPIM
                     DGSAAPFVFLLQSAGIEEQGAPKRFIRVLKRVEVQEGDKWVRFDPHDGFRLNVEIDFE
                     HPALKKYRQAMTMDFSTQSFLKEISRARTFGFLKDLEGARARDRALGGSLDNAIVMDD
                     YRVINEDGLRYRDEFVRHKVLDALGDLYLLGSSLLGEFSGYKCGHKLNNLLLRALLAE
                     PSAYESVVFEGDAQRSPVSYQLATSH"
     gene            complement(2052..2219)
                     /locus_tag="VFL84_01110"
     CDS             complement(2052..2219)
                     /locus_tag="VFL84_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607248.1"
                     /translation="MLPAEEAAHIVSAATNEAGELVLVMDTPGWAARVRYCLRALPGG
                     DVKIRVLPRSG"
     regulatory      2403..2488
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2586..3509
                     /locus_tag="VFL84_01115"
     CDS             2586..3509
                     /locus_tag="VFL84_01115"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7607249.1"
                     /translation="MQLILLSKDRGQLGNVRLTSGRVWLGLVAVTLLVAASAFYGGIT
                     AARAFGISNPHAQVDTWREELAQQQAIVDSTRRTLQQNIDALALRIGQMNAHVVRLDA
                     LGTRLTQMAGLKDGEFDFTSAPSLGGPEEPILDTEAMQINGVVGALDVLDEQLLDRSR
                     QLTVLEDLLLNRKLGDEVRPEGRPVTAGYVSSQFGGRTDPFTGRRAFHKGVDFAGREG
                     AEVVAVASGVVIWSGDRYGYGQLVEINHGNGYVTRYAHNVDNLVAVGDTVRRGQVIAR
                     MGDTGRATGPNLHFEVLLNDKPVDPLTYIGK"
     gene            3631..>5335
                     /gene="secA"
                     /locus_tag="VFL84_01120"
     CDS             3631..>5335
                     /gene="secA"
                     /locus_tag="VFL84_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET7607250.1"
                     /translation="MVGVFGSRNQRLLKRYATLVAAANALEDGLKGLSDDALRAKTDE
                     LRQRYTESQDLDALLPEAFALVREAARRVLGMRHFDVQLIGGIALHQGKIAEMRTGEG
                     KTLVATLNAYLNALTGKGVHVVTVNEYLAQRDADWMGPVYRFLGLTVGVIKSNQPTEE
                     KRAAYAADITYGTNNEFGFDYLRDNLAFRPEDRVQRTLNYAIVDEVDSILIDEARTPL
                     IISGRSEESTELYVRINQLIPSLKVHKPPAGNANDEIESTQVELIDLKGNSLGVMSTA
                     AALAKAKESGLHLIEFEPEAKPPKAQLFVPGHYSIDEKAKQAHLTEEGHEYVEKLLLK
                     AGLLAEGESLYDPSNIRLMHHLTAALRAHGLFKREVDYIVKDGEVIIVDEFTGRTMIG
                     RRWSDGLHQAVEAKEGVRVKEENQTVASITFQNYFRMYKKLAGMTGTADTEAYEFQQI
                     YGLEVVVIPTHMPMIRDDAPDLVYLTQKDKFDAIIEDIKDCNTRGQPVLVGTTSIETS
                     EFLSGLLKKQKIPHQVLNAKFHEREAEIIMQAGRPGTVTIATNMAGRGTDIKLGGNLE
                     AE"
BASE COUNT          984 a         1742 c         1729 g          880 t
ORIGIN      
        1 ttcatcacgg cgggcttggg cggcggcacg gggaccggcg cggcgcccgt cgtcgcgcag
       61 ctcgcgaagg agatgggcat cctcacggtc gccgtcgtca cgaagccgtt cggcatggaa
      121 gggccgaagc gcatgcgcgt ggcggagcag ggcatcgcgg agctcggccg gttcgtcgat
      181 tcgctgatca cgattccgaa cgagaagctg ttgtcggtgc tcgggccctc gacgacgctg
      241 ctcgacgcgt tccgttcggc gaacgaagtg ctgcagggtg ccgtgcaggg aattgccgag
      301 ctcatcacgc ggccgggcct catcaacgtc gacttcgccg acgtgcgcac cgtgatgtcg
      361 gagatgggca tggcgatgat gggctccggc gtcgcgagcg gtcaggaccg cgcgcggacc
      421 gcggccgagg cggccgtgtc gagcccgttg ctcgaggaca tcaacttgtc gggcgcgaac
      481 ggcgtgctcg tcaacgtcac ggccggcgtc gatctgtcga tcggcgagtt ccaagaggtc
      541 ggcgataccg tcaagcagtt cgcgtcggac gacgcgaccg tcgtcatcgg cacagtgatc
      601 gaccccgaga tgaccgacga gatccgcgtc actgtcgtcg cgacgggctt gggccaagct
      661 atcggcatgg cggagcgtcc gatgaaggtc gtgcgcaggg cagcgccgcc ggcgccgaag
      721 ggccgcgagc cgaactacgc ggagctcgag cggagacctg ccgttgagcg caaacgcgcc
      781 gttggcgacg gcgtgacggg caacgacagc ctcgaggact tgctcgatat cccggccttc
      841 ctgcggcggc aggcggactg aggcgcggcg cgggagccgc ttcagttcct tgctgccgcc
      901 gatggcgcta tggtaggttc gtcggccgtt tcgcgacccc ctgcgcgtcg ccgacggtct
      961 aaccccgcgc cgctcatggc tgggcggcgt agtgaaactt ctaggagcga cggctagcgt
     1021 gcgtcaacgc actctcaagg aagcgattcg cgcctcgggc atcggcctgc actcgggcga
     1081 gaaggtctac atgaccttgc gccccgcgcc ggccaatacc ggcatcgttt tcaggcgcct
     1141 cgatctgccc gagcccgtcg acgttccggc cgaggcaaca cgcgtgacgg agacgacgct
     1201 cggcacgacg ctcgagcgcg agtccgcgag gatcggcacc gtcgagcatt tgatgtcggc
     1261 catggcgggt ctcggtatcg acaatgcgtt cgtcgacttg accgccgccg aagtgccgat
     1321 catggacggc agcgctgcgc cgttcgtgtt cttgctgcag tccgcgggta tcgaggagca
     1381 gggcgcgccg aagcgtttca ttcgcgtgct gaagcgcgtc gaggtgcagg aaggcgacaa
     1441 gtgggttcga ttcgaccccc atgacggctt ccgcctgaac gtcgagatcg atttcgagca
     1501 ccccgcgctg aagaaatacc gtcaagcgat gacgatggat ttctcgaccc agtcgttctt
     1561 gaaggagatc agccgcgcgc gcacgttcgg tttcctgaag gacctcgaag gagcccgcgc
     1621 acgagatcgc gcgctcggcg gcagcctcga caacgcgatc gtcatggacg attaccgcgt
     1681 catcaacgag gacggcctgc gataccgcga cgagttcgtt cgtcacaagg tcttggacgc
     1741 gctcggcgac ttgtacctgc tcggctcgtc gctgctcggc gagttttcgg gctacaagtg
     1801 cggccacaag ctcaacaatc tgctgctgcg cgcgctgctc gccgaaccga gcgcatacga
     1861 atccgtcgtc ttcgaaggcg acgcgcagcg ctcgcccgtc tcctatcagc tcgccacgag
     1921 ccactaacca aaaaggggac agatgggaca gatctatttt ccaacaaaac ggggacagat
     1981 ctatttttcg agaacagagc cgtcgctttt gttgccgaaa ataaatccgt cccctttttc
     2041 gccgtccatt tttagccgct tcgcggcagg acgcgaatct tgacgtcccc gcccggtagc
     2101 gcgcgcaggc aatagcgcac gcgcgcggcc cagcccggcg tgtccatgac gagcacgagc
     2161 tcgccggctt cgttcgtcgc ggcgctgacg atgtgcgccg cttcctctgc gggaagcaac
     2221 tttcgaacgc gagctgtctc caatcggcga cgctcggact cgcgtatgac gcgcgccacc
     2281 ggacctgcgt tcaggagttc tgacaaatgg cgcggctcgt ccggcatggc ttctgagacc
     2341 aacgtcaacg tttgacacca agaaccttgg cttggaccgg gtttagctgc attctggccg
     2401 tgctcgatga aaggcaaacc gttcgaaaga gcgggacgca aagccaccga tctgccggag
     2461 tctctccaat gatagcgggg ttgccgggtg acttttgaca aatcgtccct ctatactcgc
     2521 ggtgcatgcc ggcgcctgcc ctggcgctgg atcgaagaat tagaagccag gagctgaggc
     2581 accgcatgca gttgatactc ctgtcaaagg atcggggaca gctgggcaac gtacgcttga
     2641 cctcgggtcg cgtctggctc gggctcgtcg ccgtcacttt gctcgtagct gcatcggcct
     2701 tctacggcgg catcacggcg gcgcgcgcgt tcggcatcag caacccgcac gcgcaggtcg
     2761 acacgtggcg cgaggagctc gcgcagcagc aggcgatcgt cgacagcacg cgccgcactc
     2821 tgcagcagaa catcgacgcg ctcgctctac ggatcggcca aatgaatgcc cacgtcgtgc
     2881 gcctcgacgc gctcggcacg cgcctgacgc agatggccgg cctcaaggac ggcgaattcg
     2941 acttcacttc cgccccgtct ctcggtggac ccgaggagcc gatcctcgat accgaggcga
     3001 tgcagatcaa cggcgtcgtc ggcgcgctcg acgtgctcga cgagcaactc ctcgatcgca
     3061 gccggcagct caccgtgctc gaggatctgc tgctgaaccg caagctcggc gacgaggttc
     3121 ggccggaggg caggcccgtc acggcgggct acgtctcgtc gcagttcggc ggccgcacgg
     3181 acccgttcac gggccgccgc gcgttccaca agggcgtcga cttcgccggc cgcgagggtg
     3241 ccgaagtcgt cgcggtcgcg tccggggtcg tgatctggtc cggcgaccgc tacggttacg
     3301 gccagctcgt cgagatcaac cacggcaacg gctacgtgac gcgctatgcg cacaacgtcg
     3361 acaacctcgt ggccgtcggc gacacggtgc gccgcggcca ggtcatcgcg cgcatgggcg
     3421 acacgggccg tgccacgggc ccgaacctcc acttcgaggt tttgctgaac gacaagcccg
     3481 tcgacccttt gacctacatc ggcaagtaag ccgccattaa cacgccgtaa cctgtcagcc
     3541 ggtgcccaaa accggcccca gtccttagaa taggcggctt tagcgctgcc cttaggaccc
     3601 tcccccgtgc tcagcaccgt ctcgaatttc atggtcggcg tgttcggcag ccgcaaccag
     3661 cggctgctca aacgctacgc aacgctcgtc gccgctgcca acgcgctcga ggacgggctt
     3721 aagggcttga gcgacgatgc gctgcgcgcc aagacggacg agctcaggca gcgctatacg
     3781 gaatcgcagg acctcgatgc gctgctgccc gaggcgtttg cgctcgtccg cgaggccgcg
     3841 cggcgtgtgc ttggcatgcg ccacttcgac gtgcagctga tcggcggcat cgcgctgcac
     3901 cagggcaaga tcgccgagat gcgcaccggc gagggcaaga ccctcgtcgc gacgctgaac
     3961 gcttacttga acgcgctgac cggcaagggc gtccacgtcg tcacggtcaa cgaatatctc
     4021 gcgcagcgcg acgccgactg gatggggccc gtgtaccgct tcctcggtct cacggtcggc
     4081 gtcatcaaga gcaaccagcc gaccgaggaa aagcgcgctg cgtacgcggc cgacatcacg
     4141 tacggcacga acaacgagtt cgggttcgat tatctacgcg acaacctcgc gttccgcccc
     4201 gaggaccgcg tgcagcgcac gctgaattac gcgatcgtcg acgaggtcga ctcgattctg
     4261 atcgacgaag cccgcacgcc gctgatcatc tcgggccgtt ccgaggagag cacggagctc
     4321 tacgtccgta tcaatcagct gatcccctcg ctgaaggtcc acaagccgcc ggccggcaac
     4381 gccaacgacg agatcgagtc gacgcaggtc gagctcatcg acttgaaggg caactcgctc
     4441 ggggtcatgt cgacggctgc cgcgctcgcg aaggcgaagg agagcggcct tcacttgatc
     4501 gagttcgagc ccgaggcgaa gccgcccaag gcgcagctgt tcgtgcccgg tcactactcg
     4561 atcgacgaga aggcgaagca ggcgcacctg accgaggagg gccacgagta cgtcgagaag
     4621 ctgctcctga aggcggggct gctcgcggaa ggcgagagtc tctacgaccc gtcgaacatc
     4681 aggctcatgc accatttgac ggccgcgctg cgcgcgcacg gcctcttcaa acgcgaggtc
     4741 gactacatcg tcaaggacgg cgaggtcatc atcgtcgacg agttcacggg ccgcacgatg
     4801 atcggccgcc gctggtccga cggcctgcac caggccgtcg aggccaaaga aggcgtgcgc
     4861 gtcaaggaag agaaccagac ggtcgcttcg atcacgttcc agaactactt ccgcatgtac
     4921 aagaagctcg ccggcatgac gggcacggcg gacacggaag cgtacgagtt ccagcagatc
     4981 tacgggctcg aggtcgtcgt gatcccgacg cacatgccga tgatccgcga cgacgcgccg
     5041 gacctcgtgt acctgacgca gaaagacaag ttcgacgcga tcatcgagga catcaaggac
     5101 tgcaacacgc gtgggcagcc cgtgctcgtc ggcacgacgt cgatcgagac gtccgagttt
     5161 ctgtccggcc ttctgaagaa gcagaagatc ccgcaccagg tcttgaacgc gaagttccac
     5221 gagcgcgagg ccgagatcat catgcaggcc ggccgccccg gcaccgtgac gatcgccacg
     5281 aacatggccg gccgcggcac cgacatcaag ctcggcggca atctcgaggc cgagc
//
LOCUS       DASSNN010000063         6506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_15221, whole genome shotgun sequence.
ACCESSION   DASSNN010000063 DASSNN010000000
VERSION     DASSNN010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6506
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15221"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFL84_01125"
     CDS             <1..429
                     /locus_tag="VFL84_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607251.1"
                     /translation="CKTLVELGDKLREGSERPFAHALDALCHYALADDTAPLDAALKD
                     LRAADAKHRLAYTLTRAALLDVERQRPEAAIARAGEALGYAEALDRPTEIVLAHAALA
                     RARKALADTAGHAKHLAALKSLDHASVARWARDRALTLAT"
     gene            445..966
                     /locus_tag="VFL84_01130"
     CDS             445..966
                     /locus_tag="VFL84_01130"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HET7607252.1"
                     /translation="MTANLFLERTFDEPLTREDVIEGGRESAQCFDFHRVRWHGSFLA
                     RDGRTMICRFSAADMESARLALRDPDTDLSRLWSGTVHKSGKPGAPSVVVERAFASPV
                     RFEDIAAIGLGKPWCWETHRVTHTHSFFSLDGKRMLCFYDAPDAEAVRVAQREAAMPA
                     SAVWAGTLVTPQR"
     gene            1078..1638
                     /locus_tag="VFL84_01135"
     CDS             1078..1638
                     /locus_tag="VFL84_01135"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET7607253.1"
                     /translation="MNVTAIKVDNGVNVEALLGARAALTEAPEAAHFKWRATCDWVRG
                     THSRTTVQGFYGLGAEQSRNKSFRYDADHPELFAAEDNGATPVEYVLIALASCLTAGI
                     ASVAQNRNIQLRSVRATLEAGMDMQGILGIDSEVRNGFDGIKVVFHIDADATRDEINA
                     LVAQSQRRSAVFDIVTNPTFVAVEVA"
     gene            1638..2942
                     /locus_tag="VFL84_01140"
     CDS             1638..2942
                     /locus_tag="VFL84_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET7607254.1"
                     /translation="MSGAARRPRVTGIVDAVVVGAGHSGLAMSRALAAHGVEHVVLER
                     GEIANAWRRERWDSLRLLTPNWQSRLPGYRYAGPDPDGYMTSAEVAEFIGGYARVVGA
                     PVRSGTTVTKLRAIDGGYVVETDRGAWRCRAAVLASGAHELPVVPEIAARLPSGIRTL
                     TAKQYRNPAALDPGRVLVVGASATGLQLADEIRRSGRPVTIAVGEHIRMPRLYRGRDV
                     QWWLEAVGILDERYDEVDDIVRARRVPSPQLLGSTERRTLDLNALIELGVDVVGRLVG
                     VDDTRLQFSGSLANHCAMADLKLNRLLERIDRWVDEHRVGDMVAVPERLGPTRIEATP
                     RLVLDSAKEPIATVIWATGFRPDYSWLEVAALDTKGRLRHDGGVAAAPGLYALGLNFM
                     RRRKSSYMHGAEDDVRDLSDHLVRHLRGSARRRPAQAAGGNR"
     gene            3019..5490
                     /locus_tag="VFL84_01145"
     CDS             3019..5490
                     /locus_tag="VFL84_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607255.1"
                     /translation="MDAPLNDLRQALRALRKSPVFSATVVLTLALGIGANTAIFSVFS
                     AVVLQPLPFPEADRLVQLVNTSADGTPGDTGVSPIVYRHFREQTDVLEDVAAYRDAWV
                     NYSAGDVLERLAATQVTEPYFRAFRASMALGRPFTADEDLPGAAKTAIVSYDFWMRRL
                     GGETAAIGSTLRLNGVVHTVVGVAAEAFDTRKLGRTEVWIPLQLTSTGTDGGATLAAA
                     ARLKAGVSLQQAQARLAASTASFDRVAVGLRDGLFGAVSFKEARIATASGSLFRNDPR
                     NVLWLLFGAVSCVLLIACANVASLMLVRASAREREIAVRTALGAGRWRIVRQLLAESA
                     ILSAAGGAFGLLLGFGGIRALSSASASGLSWLGVDGALLGVDSRVVAFTMAVSMVTVI
                     ISGLLPALITSRADPNTVIKQASGLSATARGRNKSRSILVVAEVGVAVVLLIGAGLLI
                     KTTAALNATDPGVNVEDVVVMRTLLSEERFLAANATRELVRGTLERIRAIPGVEAAGA
                     TCCVPLQGSWGDVFKVVGRDDGGRPFTSGGDVTVSTGDYFDVFEIAVLRGRAFDDRDN
                     AGGAPVIVINRALADRWWPNGEDPIGQKMRVGGSHDEPEREVIGIVENVRQARLELVR
                     QTMYVPFAQLSDTWHQGLLPDDALAWIIRTSTDPLGTAAPIRREIERSTGVPVTDVAL
                     MSEIVADSISRQRANMLLMSVFGGVALVLAAIGVYGLVAYSARERTHEIGIRMALGAR
                     GERIVSMLLRQGLALVAGGVVLGVTAAYSLAGVLASILYGVEPRNTGVFVVAPVLLAL
                     VALAAISVPAYRASRVDPLQALRQE"
     gene            complement(5534..6079)
                     /locus_tag="VFL84_01150"
     CDS             complement(5534..6079)
                     /locus_tag="VFL84_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7607256.1"
                     /translation="MRRKDWANFGDFIAGGFGPGFPGGRRHRRWGRWFEPGEMRLVIL
                     RLVREKPRHGYEIMKALEERMHGCYTPSAGSVYPTLQLLEDQGYVRIVGEDGKKVYHI
                     TPEGERFLDENEDALTDIGERIKETVRGVAGGAVADVNAAFAQLAKRVFTTAWRGGPD
                     SERTAKIAEILRRATADIEAL"
     gene            6216..>6506
                     /locus_tag="VFL84_01155"
     CDS             6216..>6506
                     /locus_tag="VFL84_01155"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET7607257.1"
                     /translation="MAKVTSKLQVTIPKHIADEYGIVPGDEIDFVPAGDSIRVIPPRR
                     RPQTMLSLEERLRLFDESRARQREREKNMVLPKDPPKERDWKREDLYTRGKPR"
BASE COUNT         1015 a         2197 c         2223 g         1071 t
ORIGIN      
        1 tgcaagacgc tcgtcgagct cggcgacaag ctgcgcgagg gcagcgagcg gccattcgcg
       61 cacgcgctcg acgcgctctg tcattacgcg ctcgctgacg acaccgcgcc gctcgacgcc
      121 gcactgaagg acttgcgcgc ggccgacgcc aagcaccggc tcgcctacac gttgacgcgg
      181 gcggcgctgc tcgacgtcga gcggcagcgg ccagaagccg cgatcgcgcg cgcgggcgaa
      241 gcgctcggct atgcggaagc gctcgatcgg cccacggaga tcgtgcttgc gcatgcggcg
      301 ctcgcgcggg ctcgcaaagc cctcgcggat accgcgggac atgccaagca tctcgcggcg
      361 ctgaagtcgc tcgaccatgc atcggtcgcg cgctgggcgc gagatcgcgc gctgacgctg
      421 gccacgtaga agaggagacg gaccatgacg gcgaatctat tcctcgagcg cacgttcgac
      481 gagccgctga ctcgcgaaga cgtcatcgaa ggcggccgtg aatccgcgca gtgcttcgat
      541 tttcaccgcg tccgttggca cggcagcttc ctcgcacgcg atggccgcac gatgatctgc
      601 cggttctccg cagcggacat ggagtcggcg cgcctcgcgc tacgcgatcc cgacacggat
      661 ctgtcgcggc tttggtccgg cactgtgcac aagagcggga agcccggcgc gccgagcgtc
      721 gtcgtcgagc gcgcgttcgc gtcccccgta cgcttcgagg acatcgcggc gatcgggctc
      781 ggcaagccgt ggtgctggga aacgcaccga gtcacgcata cgcattcgtt cttctcgctc
      841 gacggcaagc gcatgctgtg cttctacgac gcgcccgacg ccgaggccgt gcgcgtcgct
      901 caacgcgaag cggccatgcc ggcctcggcc gtctgggccg gcaccctcgt gaccccgcaa
      961 cgttaggcac cacgcaaatt ccacggccgc ttccacgctc ggtccaacgg ccgtcgcgca
     1021 tcgttccttc ccgtccccgg ccgccggccg gatcaaccac gagacgagga acatgcaatg
     1081 aacgtcaccg caatcaaagt cgacaacggc gtcaacgtcg aagcactgct cggcgcgcgc
     1141 gccgcgctca cggaggcgcc cgaggccgcc cacttcaagt ggcgcgcgac gtgcgactgg
     1201 gtcaggggca cgcatagccg cacgaccgtg caaggctttt acggcttggg cgccgagcag
     1261 tcgcgcaaca agtcgttccg ctacgacgcc gaccatcccg agctcttcgc cgccgaagac
     1321 aacggcgcaa cgccggtcga gtacgtgctg atcgcgctcg cgtcgtgcct gacggccggc
     1381 atcgcttccg ttgcgcagaa ccgcaacatt cagctgcgct cggtccgcgc gacgctcgag
     1441 gccggcatgg acatgcaggg catcctcggt atcgacagcg aggtacgcaa cggtttcgac
     1501 ggcatcaagg tcgtcttcca catcgacgcc gatgcgacgc gcgacgagat caacgcgctc
     1561 gtcgcgcaat cgcagcgccg ttccgccgtg ttcgacatcg tgacgaaccc gactttcgtc
     1621 gcggtcgagg tcgcgtgatg agcggcgccg cgcgccgccc aagggtgacc ggcatcgtcg
     1681 acgccgtcgt cgtcggtgcc gggcattccg gcctcgcgat gagccgcgcg ctcgccgcgc
     1741 acggcgtcga gcacgtcgtg ctcgagcgcg gcgagatcgc gaacgcgtgg cggcgcgagc
     1801 gctgggactc gctgcggctc ttgacgccga actggcagag ccgcctgccg ggctaccgtt
     1861 acgcgggccc cgatccggac ggctacatga cgtcggccga ggtcgcggaa ttcatcggcg
     1921 ggtacgcaag agtcgtcggc gcgcccgtgc gcagcggcac gacggtcacg aagctgcgcg
     1981 ccatcgacgg cggctacgtc gtcgagacgg accgcggcgc atggcgctgc cgcgccgcag
     2041 tgctcgcgag cggcgcccac gagctgcccg tcgtgcccga gatcgcggcg cgcttgccga
     2101 gcggcattcg aacgttgacg gccaagcagt accgcaatcc ggcggcgctc gatcccggcc
     2161 gtgtgctcgt cgtcggcgct tcggcgacgg ggctgcagct cgccgacgag atccggcgct
     2221 cgggccggcc cgtcacgatc gcggtcggcg agcacattcg catgccgcgt ctctatcgcg
     2281 gccgcgacgt ccagtggtgg ctcgaagccg tcggcattct cgacgagcgc tacgacgagg
     2341 tcgacgacat cgtgcgcgcg cgccgcgtgc cgtcgccgca gctcctcggc tcgacggagc
     2401 ggcgcacgct cgatttgaac gcattgatcg agctcggcgt cgacgtcgtc ggccgcctcg
     2461 tcggcgtgga cgacactcga ctgcaatttt cgggctcgct cgcgaatcac tgcgcgatgg
     2521 cggaccttaa gctgaatcgc ttgctcgagc gcatcgaccg ctgggtcgac gagcaccgtg
     2581 tcggcgacat ggtcgcggtg ccggagcgat tggggccgac gcgcatcgag gcgacgccgc
     2641 ggctcgtgct cgactccgcc aaggagccga tcgcaaccgt catttgggca acgggttttc
     2701 gtccagacta ttcatggctc gaagtggcgg cgctggacac gaagggtcgg ctgcgtcacg
     2761 acggcggcgt cgcggcggcg cccggtctct acgcgctcgg cctgaatttc atgcggcggc
     2821 gcaagtcgag ctacatgcac ggcgccgagg acgacgtgcg cgacttgagc gaccacctcg
     2881 tgcggcactt gcgcggctcc gcgcgtcgac gtcccgcgca ggcggccggg ggcaatcgct
     2941 agcagaccgc tcctcgggca aggagcgcat gacgccgtcg cttgcccgcg ccacgcgtgg
     3001 cgcgtaggtt ggcgggccat ggacgcgccg ctcaacgacc tgaggcaggc cttaagagct
     3061 ctgcgcaaga gtcccgtttt ctcggcaacc gtcgtcctca cgctggcact gggcatcggc
     3121 gcgaacacgg cgatcttctc ggtgttcagt gcggtcgtgc ttcagcctct gccgttcccc
     3181 gaggcggacc ggctcgtgca actcgtgaac acgagtgccg acggcacacc aggcgatacc
     3241 ggtgtgtcgc caatcgtgta ccggcacttc cgcgagcaaa ccgacgtctt ggaggacgtc
     3301 gccgcttacc gcgacgcctg ggtcaactac tcggccggcg acgttctcga gcggctcgcc
     3361 gcgactcagg tgacggaacc gtatttccgc gcgttccggg cctcgatggc gttgggccga
     3421 ccgttcaccg ccgacgagga cttgccgggc gcggcgaaga cggcaatcgt cagctacgac
     3481 ttctggatgc ggcggctcgg cggggagacg gccgcgatcg gcagcacgct gcgactcaac
     3541 ggcgtcgtgc acactgtcgt cggcgtcgcc gcagaggcgt tcgacacgcg caagctcggc
     3601 cgcaccgaag tctggattcc gctgcagctg acgtcgaccg gcaccgacgg tggagccacg
     3661 ttggcggctg ccgcgcggct gaaagcgggc gtgtcgttgc agcaagcgca agcgcgcctc
     3721 gccgcgtcga cggcgtcctt cgatcgtgtc gccgtcgggc tgcgcgacgg cctgttcggc
     3781 gcggtgtcgt tcaaagaggc gcgcatcgca acggcgtcgg gctcgctgtt ccgcaacgac
     3841 ccccgcaacg tcctctggct gctgttcggc gccgtctcgt gcgtactgct gatcgcgtgc
     3901 gcgaacgtgg cgagcttgat gctcgtgcgc gcgagcgcgc gcgaacgcga gatcgccgtt
     3961 cgtacggcgc tcggcgcggg acgctggcgg atcgtgcggc aactgctggc ggaaagcgcg
     4021 atcttgtccg ccgccggcgg cgcgttcggc ttgcttctcg gcttcggcgg aatccgtgcg
     4081 ctgtcgtctg cgagcgcgag cggcttgtct tggctcggcg tggacggcgc gctgctcgga
     4141 gtcgactcca gggtcgtcgc gttcacgatg gccgtgtcga tggtgacggt catcatctcc
     4201 ggcctgttgc ccgcgctgat cacgtcgcgc gccgacccca acaccgtgat caagcaggcg
     4261 agcggcctgt cggcaaccgc gcgcggtcgg aacaagagcc ggtcgattct cgtcgtcgcg
     4321 gaagtcgggg tggccgtcgt tcttctgatc ggcgccggac ttctgatcaa gacgacggca
     4381 gccctgaatg cgacggatcc tggcgtcaac gtggaggacg tcgtggtcat gcgcacgcta
     4441 ctgtcggagg agcgattcct cgcggcgaac gcgacgcgcg agctcgtgcg cggcacgctc
     4501 gagcgaatac gcgcgatccc gggcgtcgaa gccgcggggg caacgtgttg cgttccgctg
     4561 cagggcagct ggggtgacgt tttcaaggtc gtcgggcgag acgacggcgg caggccgttt
     4621 acgagcggcg gcgacgtgac ggtcagcacc ggcgactact tcgacgtctt cgagattgcc
     4681 gtcttgcgcg gccgcgcatt cgacgaccgc gacaatgcgg gcggcgctcc cgtgatcgtc
     4741 atcaaccgtg cgctcgccga ccggtggtgg ccgaacggtg aagacccgat cggccagaag
     4801 atgcgggtcg gcggcagcca cgacgagccc gagcgcgagg tgatcggcat cgtagagaac
     4861 gttcgccagg ctcgcctgga gctcgttcgc caaacgatgt atgtgccgtt cgcgcagctc
     4921 tccgatacat ggcaccaggg cctgctgccc gacgacgcgc ttgcgtggat tattcgaacc
     4981 agcacggatc cgctcggcac tgccgcgccg attcggcgcg agattgaacg aagcacgggc
     5041 gtgcccgtca cggacgtcgc gttgatgagc gaaatcgtcg ccgactcgat ctcgcgacag
     5101 cgagccaaca tgctgctgat gagcgtattc ggcggcgtcg cgttagtgct cgcggcgatc
     5161 ggcgtctacg ggctcgtcgc ttactcggct cgtgagcgaa ctcacgaaat tggaattcga
     5221 atggcgctcg gtgcccgagg cgagcgcatc gtcagcatgc tgcttcgaca aggtctcgcg
     5281 ctcgtcgcgg gcggcgtggt gctcggcgtg acggcggcgt attcgttggc cggcgtgctc
     5341 gcgtcgattc tgtatggcgt cgagccgaga aacacggggg ttttcgtggt tgcccccgtc
     5401 ctgctcgccc tcgtagctct tgctgcgatt tcggttcccg cgtatcgagc gagccgagtg
     5461 gacccgcttc aggcattgcg ccaggagtag tcgccgacgc ggcgaaccgc gaaaacaccg
     5521 ctacgtctcg ggctcagagc gcttcgatgt cggccgtggc tcggcgcagg atctcggcga
     5581 tcttcgccgt ccgctcgctg tcgggcccac cgcgccaggc agtcgtgaac acacgcttcg
     5641 cgagctgcgc gaacgcggca ttgacgtcgg ccaccgcgcc gcccgcgacg ccgcgcacgg
     5701 tttccttgat gcgctcgccg atgtcggtca gcgcgtcctc gttctcgtcg aggaaccgct
     5761 cgccttcggg cgtgatgtgg tagaccttct tgccgtcctc cccgacgatg cgcacgtacc
     5821 cctggtcctc gagcagctgc agcgtcggat agacgctgcc ggcggacggc gtatagcagc
     5881 cgtgcatgcg ctcctcgagc gccttcatga tctcgtagcc gtgccgcggc ttttcgcgca
     5941 cgagccgcag gatcacgagc cgcatttcac cgggctcgaa ccagcggccc cagcggcgat
     6001 gccgacgtcc gcccggaaag ccggggccga atccgcccgc aatgaagtcg ccgaaattgg
     6061 cccagtcctt acgcctcatg gggttagctc cttacgatag atcgtgcgct atatcgcggc
     6121 gacatagcgt gcgatatatc gttgctgcca gcaagcgatg gatcgagcgg cgccgcagct
     6181 ttgagcaagg atgctgtcct tgctaaagta aggccatggc caaagtcacg agcaagctcc
     6241 aggtcacgat cccgaagcac atcgccgatg agtacgggat tgtgccgggc gacgagatcg
     6301 acttcgtgcc ggccggcgac agcatccgcg tgatccctcc acgccggcgc ccgcagacca
     6361 tgctgagcct cgaggaacga ctgcggttgt tcgatgaatc gagagcgcgg cagcgcgagc
     6421 gcgagaaaaa tatggtcctt cccaaggacc ctccgaaaga gcgcgactgg aaacgagagg
     6481 atctgtacac gcgtggcaag cctcgt
//
LOCUS       DASSNN010000064         4982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1528448, whole genome shotgun sequence.
ACCESSION   DASSNN010000064 DASSNN010000000
VERSION     DASSNN010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528448"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VFL84_01160"
     CDS             <1..361
                     /locus_tag="VFL84_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607258.1"
                     /translation="KGQVVAWNVDDLHEDRNNIRKPTALDEKGEEVNGVGDQPNQHDI
                     LTGSDSTGRLVPGNVTLTTCNNWTSNSAGNAMLGHHDRLGGPNASWNSVHSSRSCSQA
                     DLVATGGAGLFYCFAAQ"
     gene            481..1218
                     /locus_tag="VFL84_01165"
     CDS             481..1218
                     /locus_tag="VFL84_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607259.1"
                     /translation="MLRTLNKAVMVASALTLLVAFFNRNDLPGTIDFDPKLKGEPRQR
                     ETHKPPVTMSYGGVQYRVEPQYDYELYGLVVSYRQHDGESSMHRWSNDHLNMADVCVV
                     WSDTAFSPTLSRLDFWNGIFTCNVQTRDSVAWSNFKMNQLSNNHLISASPGIRDRVAE
                     IRIGDQIRIKGSLSRYGAVGGGGMRGTSTTREDTGDGACETILVDEFEILVPAFSPWR
                     AAMWTSLAMLIGALAVHFMMPYRPYAD"
     gene            1245..2702
                     /locus_tag="VFL84_01170"
     CDS             1245..2702
                     /locus_tag="VFL84_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7607260.1"
                     /translation="MSGFTGKNLIGGEWRAAASGRAFEQRNPAFLHEVTGTFADSSAE
                     DVDAAIAAAQAAFPAWRALSPLARKAYLDRALRSMIARRDEIAVVITRENGKALREAR
                     GEVDSAIKEMDYQIAEGVRLCGETVPVEVGGTLAYSTREALGPVAIVTPWNFPFNVPC
                     RKGTPALMGGNTIVLKPASLTPRTGLLFAELLAESGLPPGVVNCVTGAGGSVGAALVG
                     DARIKAVSFTGSTPVGKSIQLAAARNLTPTQLELGGKNPAIVLPDADLDLTVAEIVKG
                     AFACSGQWCTSTSRVIVLEEIAEELTRRIVAAAKALAVRDGTAEDCGMGPVCGAKQKV
                     DILAYIAKGRAEGARLLTGGAALDGEDYDHGCFIAPTVFDRVTPEMTIAREEIFGPVL
                     AIMTVRSFEEAVDVANGVAYGLSSSIFTNDLRRALTYMERAEVGLAHVNLMTALKEPQ
                     LTFGGVKESGAGLPEAGSSGIEFFTHHKVCYVKYR"
     gene            complement(2716..3204)
                     /locus_tag="VFL84_01175"
     CDS             complement(2716..3204)
                     /locus_tag="VFL84_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family B"
                     /protein_id="HET7607261.1"
                     /translation="MTTIRACRPTDAAAIAAIYNHYVRETVVTFEETPVAAAEMAQRM
                     EDVGARFPWLVWDDGDAVVGYAYAAAWKTRAAYRLSVETTIYLAESHVGRGIGSKIYA
                     ALLDEIRERGFHSAIGGVALPNPASVALHEKLGFKKVAQFAEVGRKFDRWIDVAYWQL
                     IF"
     gene            3394..>4982
                     /locus_tag="VFL84_01180"
     CDS             3394..>4982
                     /locus_tag="VFL84_01180"
                     /inference="COORDINATES: protein motif:HMM:NF026288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7607262.1"
                     /translation="MSAARVSRGSVRYALCIACCCATVASVSAQSPPAAVQLHEGRPQ
                     TASQSTEDRFRYDVDIGAGTYVVRLEQRGLDLKLAVESPGGATETHDSPTLRDGEEIA
                     VLTGPGRYRVDVYSDESTDARGGHAIALVNASNAAANELEAWRLAGAGAAANFAGGEE
                     GWSKAADAYARAAELWHGLGRTREEADALFAAATVQYRQLVAWQTAAEQAARAAALYE
                     QLGDAALAANALHLQGASLVEQALEVRQSAEADAPLAAESEELFGEALRLLEQARATH
                     ERLGRLYDLGKVVNNFGYTYFSRGEFARARPYYEQAAALFASAGEWGAQVQPLANAAV
                     LDAEAGRLASAIATFEQMLKIMPPGKLLARRSVTLENLGATQLLRGDAEIALRTFADA
                     LALYRVREDEHGESRALRRIGEAYYTLGELDLAEQYLRQALPLAERTNEARTQEAALR
                     NLGNVAFLRRDYAAALELHRRALESSASLSDRAYLQVLVTKDLVALGRAAEARAAAGE
                     ARAFAQSSGSERLLAAADAAVG"
BASE COUNT          803 a         1705 c         1726 g          748 t
ORIGIN      
        1 gaagggccag gtcgtcgcgt ggaacgtcga cgatctgcac gaggaccgca acaacatccg
       61 caagccgacc gcgctcgacg agaagggcga ggaggtcaac ggcgtcggcg atcagccgaa
      121 ccagcacgac atcctcacgg gctcggactc gaccggccgg ctcgtgccgg gcaacgtcac
      181 gctgacgacc tgcaacaact ggacgagcaa cagcgcgggc aacgcgatgc tcggccatca
      241 cgaccggctc ggcggcccga acgcgtcgtg gaactccgtg cacagctcgc gcagctgcag
      301 ccaagcggat ctcgtcgcga cgggcggcgc cggtctcttc tactgcttcg ccgctcagta
      361 gcgattcgac ggccgccgcc gcgcgacgca taggtcccgg cggcggccgg agacgtccat
      421 cacgctcgtt tcgtgacgtc gctcggcgcg cgggcgcgcc tacgcgcgta ccgtgcgcgc
      481 atgctgcgga ctttgaacaa ggccgtgatg gtcgcgagcg cgctgacgct gctcgtcgcg
      541 ttcttcaatc gcaacgactt acccggcacg atcgacttcg atccgaagct caagggcgag
      601 ccgcgccagc gcgaaaccca caagccgccc gtcacgatgt cgtatggcgg cgtccagtat
      661 cgcgtcgagc cgcagtacga ctacgagctg tacgggctcg tcgtctcgta tcggcagcac
      721 gacggcgaga gctcgatgca tcgctggtcg aacgaccatc tgaacatggc cgacgtctgc
      781 gtcgtatgga gcgacacggc gttcagcccg accctctcga ggctcgactt ctggaacggc
      841 atcttcacgt gcaacgtgca gacgcgcgac agcgtcgcgt ggtcgaactt caagatgaac
      901 cagctctcga acaaccattt gatctcggcg agcccgggca ttcgcgatcg cgtcgccgag
      961 atccgcatcg gcgatcagat ccgcatcaaa ggctcgctgt cgcgctacgg cgcggtcggc
     1021 ggcggcggca tgcgcggcac gagcacgacg cgcgaggata cgggcgacgg cgcctgcgag
     1081 acgatcctcg tcgacgagtt cgaaatcctc gtgccggcct tcagtccgtg gcgggccgcg
     1141 atgtggacct cgctggcaat gctgatcggc gcgctcgccg tgcacttcat gatgccctat
     1201 cgcccctatg ccgattgacc ccgagccagc acaatggctg ctccatgagc ggcttcacgg
     1261 gcaagaatct gatcggcggc gagtggcggg ccgcggctag cggccgcgct ttcgagcagc
     1321 gcaatcccgc gttcctgcac gaggtgacgg gcacgttcgc cgactcgtcg gcggaggacg
     1381 tcgatgccgc gatcgcggcg gcgcaagcgg cgttcccggc ctggcgtgcg ctgtcgccgc
     1441 tcgcgcgcaa ggcctatctc gaccgcgcct tgcgctcgat gatcgcgcgc cgcgacgaga
     1501 tcgcagtcgt catcacgcgc gagaacggca aggcgctgcg cgaagcgcgc ggcgaggtcg
     1561 actccgcgat caaagagatg gactatcaga tcgccgaggg cgtgcgcctc tgcggcgaga
     1621 ccgtgcccgt cgaggtcggc ggcacgctcg catattcgac gcgcgaagcg ctcgggcccg
     1681 tcgcgatcgt gacgccgtgg aatttcccgt tcaacgtgcc gtgccgcaag ggcacgccgg
     1741 cgctgatggg cggcaacacc atcgtgctga agcctgcgag ccttacgccg cgcacggggc
     1801 tcctattcgc ggagctgctc gccgagagcg ggctgccgcc gggcgtcgtc aattgcgtga
     1861 ccggcgccgg cggcagcgtc ggcgctgcgc tcgtcggcga cgcgcgcatc aaagccgtct
     1921 cgttcacggg ctcgacgccg gttggcaagt cgatccagct cgcggccgcc aggaatctca
     1981 cgccgacgca gctcgagctc ggcggcaaga atcccgccat cgtgctcccg gacgccgacc
     2041 tcgatctcac cgtcgccgag atcgtcaaag gcgcgttcgc atgctcgggc cagtggtgca
     2101 cgtcgacgag ccgagtcatc gtgctcgagg agatagccga agagctcacg cggcggatcg
     2161 tcgccgctgc gaaagcgctc gccgtccgcg acggcacggc ggaagactgc ggtatgggcc
     2221 cggtctgcgg cgcgaagcaa aaagtcgaca tcttggccta catcgcgaaa ggccgcgcgg
     2281 aaggcgcgcg cctgttgacg ggcggcgccg cgctcgacgg cgaagactac gaccacggct
     2341 gcttcatcgc gccgacggtg ttcgatcgcg taacgcccga aatgacgatc gcgcgcgagg
     2401 agatcttcgg ccccgtgctc gcgatcatga cggtgcgaag cttcgaggag gccgtcgacg
     2461 tcgcgaacgg cgtcgcgtac ggcctctcgt cgtcgatctt cacgaacgac ctgcggcgcg
     2521 cgttgaccta catggagcgc gccgaggtcg gcctcgcgca cgtgaatctg atgacggcgc
     2581 tgaaagagcc gcagctgacc ttcggcggcg tcaaggaatc gggcgcggga ttacccgaag
     2641 cgggctcgtc cggcatcgag ttcttcacgc accacaaagt ctgctacgtg aaataccgct
     2701 gacgcggctc ggcgctcaaa agatcagctg ccagtaagcg acgtcgatcc aacggtcgaa
     2761 cttgcggccg acctcggcga actgcgcgac ttttttgaag ccgagcttct cgtgcagcgc
     2821 gacgctcgcc ggattcggca gcgcgacgcc gccgatggcg ctgtggaagc cgcgctctcg
     2881 gatctcgtcg agcaaggccg cgtagatctt cgagccgatc ccgcggccga cgtggctctc
     2941 cgccaggtag atcgtcgtct cgaccgatag ccgatacgcg gcgcgcgtct tccacgccgc
     3001 cgcgtaggca tagccgacga cggcgtcacc gtcgtcccac acgagccacg ggaagcgcgc
     3061 gccgacgtct tccatgcgtt gcgccatctc ggccgccgct accggcgtct cctcgaacgt
     3121 gacgacggtt tcccgcacgt agtggttgta gatcgcggcg atcgcggcgg cgtcggtcgg
     3181 tctgcaggcg cgaatcgtcg tcacggtatg ctttgcggcg gcgctacggc ggcgtcgccg
     3241 attctagccg atgaccgctg ctgccgggcg cggcacgggg cggcgcgtga gatacttccg
     3301 ccggccgcga cagctcgcct tgtgacgcct tcgagccgtt cgggccggcc ttttgccttc
     3361 gacgcacgac gacaaggcca tcgggagggg agattgagcg ccgccagggt ttcgcgaggc
     3421 tccgtccgct acgcgttgtg cattgcgtgc tgctgcgcga ccgtcgcaag cgtatccgcg
     3481 cagtcgccgc cggcggccgt tcagctccat gaaggccggc cgcaaacagc cagccagtcc
     3541 acggaggacc gcttccgtta cgacgtcgat atcggcgccg gcacttacgt cgtgcggctc
     3601 gagcaacgcg ggctcgatct gaagctggcg gtcgagagcc cgggcggcgc gaccgagacg
     3661 cacgactcgc cgacgcttcg cgacggcgag gagatcgccg ttctcacagg ccccgggcgc
     3721 tatcgcgtcg acgtctattc cgacgaaagc acggatgcgc gcggcggcca cgccatcgcg
     3781 ctcgtgaacg cgtcgaacgc cgcggcgaac gagctcgagg cgtggcgcct cgccggcgcg
     3841 ggcgcggccg ccaacttcgc gggcggcgag gagggctggt cgaaggcggc cgacgcgtac
     3901 gcgcgcgccg cggagctctg gcacggtctt ggccgcacgc gcgaggaagc ggacgccttg
     3961 tttgccgcgg ccacggtcca gtatcggcag ctggtcgcct ggcaaacggc cgcggagcag
     4021 gcggcgcgcg cagccgcgtt gtacgagcag ctcggcgacg cggctttggc cgcgaacgcg
     4081 ctgcatcttc aaggcgcttc gctcgtcgag caggcgctcg aggtgcggca gtcggcggaa
     4141 gcggacgctc cgctcgccgc agagtccgag gagctgttcg gcgaggcgct gcggctgctc
     4201 gagcaggcgc gcgccacgca cgagcggctc ggccgcctct acgatctcgg caaggtcgtg
     4261 aacaatttcg gctacacgta cttcagcagg ggagaattcg cgcgcgcacg cccgtactac
     4321 gagcaagcgg cggcgctgtt cgcgagcgcc ggcgaatggg gcgcgcaggt ccagccgctc
     4381 gcgaatgcgg ccgtgctcga cgccgaggcg ggccggctcg cgagcgcaat cgcgacgttc
     4441 gagcagatgc ttaagatcat gccgcccggc aagctgctcg cgcgccggtc ggtcacgctc
     4501 gaaaacctcg gcgcgacgca gctgttgcgc ggcgacgccg aaatagcctt gcgcaccttc
     4561 gccgatgcgc tcgctctgta ccgcgtccgc gaggacgagc acggcgagag ccgagcgctg
     4621 cgccgcatcg gggaggccta ttacacgctc ggcgagctcg acctcgcgga gcagtatctg
     4681 cggcaggctc tgccgctcgc cgagcgcacg aacgaagcga gaacgcagga agcggcgctg
     4741 cggaacctgg gcaacgtcgc gttcttgcgg cgcgactacg cggctgcgct cgagctccac
     4801 cggcgcgcgc tcgagagctc cgcctcgctc tccgatcgcg catatctgca agtgctcgtg
     4861 acgaaggatc tcgtcgcgct cggccgcgcc gccgaagctc gagcggcggc gggcgaagct
     4921 cgcgcgttcg cgcagagctc gggctcggag cggctgctcg cggccgcaga cgcggccgtg
     4981 gg
//
LOCUS       DASSNN010000065         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1528527, whole genome shotgun sequence.
ACCESSION   DASSNN010000065 DASSNN010000000
VERSION     DASSNN010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528527"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFL84_01185"
     CDS             complement(<1..333)
                     /locus_tag="VFL84_01185"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7607263.1"
                     /translation="MKRLPPSKLGAWREISRETIADCRIFTVERSIAESPVDGAPRPF
                     HRIRSVDWAQIVPITRRNEAVLVRQYRHGSQSITLEIPGGLVDPGEDPATAALRECLE
                     ETGYRARVA"
     gene            complement(330..>2234)
                     /gene="feoB"
                     /locus_tag="VFL84_01190"
     CDS             complement(330..>2234)
                     /gene="feoB"
                     /locus_tag="VFL84_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HET7607264.1"
                     /translation="DVLFGRVAGTPKPEAILAVIDSTRLYQGLYLLQQLTALGRPMIV
                     ALTMIDAAEREGRRIDFEALEEALGGVPVYPVVATSGRGFPALNAALATILAVPPPRE
                     AARWPELERAAEALVAHWRARGADLHRVEAERLLIDASAPTLVPQAGADIAAARTALF
                     GIEPPLAAEARRRYAWVLETLARVESPGSGVVAQTARLIDWINRPWPGTAALIVLMFL
                     VFQAVFAWATPLVDLIDLGFAGLGSLAAGVLPDGTWIESLVVKGIIAGVGSVIVFLPQ
                     ILILFLFINLLEDTGYLARAAFLVDRAMRSVGLSGQSVIPLLSSFACAVPSIMATRVI
                     PNRRDRIATILAAPFMTCSARLPVYALLIGAFVPALRVGFVNLQGLVLFGLYAIGIVG
                     GVATALLLRSSVLRGENPTFILALPEFRLPNVRTVAMKLLDRVRVFLKRAGTIIFAVA
                     VVVWALTYFPRSPEIEQARAAAEAEARATLSGPVLEQRLAEIGNQAAADHIAQSWLGR
                     AGHAVEPAFRPLGWDWRVSAAVIAGFPAREVVVAVLGTIYAVGEEADEETLAGRLTAA
                     QHPDGAPVFTLPMVLGLLLFYAWCLQCAATLAVIRRETNSWRWPLFAWSYMTALGYGG
                     ALLIYQLGTA"
BASE COUNT          396 a          785 c          771 g          282 t
ORIGIN      
        1 cgcgacccgc gcccggtagc ccgtctcctc gaggcattcg cgcagcgcgg ccgtagcggg
       61 atcctcgccc gggtcgacga gcccacccgg aatctcgagc gtgatgctct gcgagccgtg
      121 gcggtactgc cgcacgagca cggcctcgtt gcggcgtgtg atcggcacga tctgcgccca
      181 gtccacggac cggatgcggt gaaacggccg cggcgcgccg tcgacgggcg actctgcgat
      241 cgaccgctcg accgtgaaga tgcggcagtc cgcgatcgtc tcgcgcgaga tctcgcgcca
      301 cgcaccgagc ttcgacggcg gcaagcgttt catgccgttc ccagctgata gatgagcagc
      361 gcgccgccgt agccaagcgc ggtcatgtag ctccacgcga acagcggcca gcgccagctg
      421 ttcgtctcgc gccgaatcac ggcgagcgtg gcggcgcact gcaagcacca cgcatagaac
      481 aacagaaggc ccaagaccat cggcaacgtg aagacgggag cgccgtcggg atgttgcgcc
      541 gccgtgagtc gtccggccag cgtttcttcg tcggcttcct cgccgacggc gtagatcgtc
      601 cccagcacgg cgacgacaac ctcgcgcgcc gggaatcccg cgatgaccgc cgcggagacg
      661 cgccaatccc aaccgagcgg acggaacgcg ggctccacgg cgtgcccggc ccggccgagc
      721 caactttgcg caatgtgatc ggcggccgcc tgattgccga tctcggcgag ccgctgctcg
      781 agcactgggc cggagagcgt ggccctcgcc tcggcctccg ccgccgcgcg cgcctgctcg
      841 atctccggcg aacgcgggaa atacgtgagc gcccagacga cgaccgcgac cgcgaaaatg
      901 atcgttccgg cacgcttcaa gaacacgcgc acgcggtcga gcagcttcat cgcgaccgtt
      961 cgaacgttcg gtaaccgaaa ctccggcagc gccaggataa aggtcggatt ctcgccgcgc
     1021 agcacggagc tgcgcagcag cagcgccgtg gcgacgccgc cgacgatgcc gatcgcgtac
     1081 aaaccgaaca gcacgagccc ttgcaggttc acgaagccga cgcgcagcgc gggcacgaat
     1141 gcgccgatca gcagcgcgta gacgggaagg cgtgcggagc acgtcatgaa cggcgcggcc
     1201 aggatcgtcg cgatgcggtc gcgccgattc gggatcacgc gcgtcgccat gatcgacggc
     1261 accgcgcacg cgaagctcga cagcagcggg atcaccgact gccccgacag gccgacgctg
     1321 cgcatcgcgc ggtcgacgag gaatgccgcg cgcgcgaggt agcccgtgtc ttcgaggaga
     1381 ttgatgaaca agaacaggat caggatctgc ggcagaaaga cgatgacgct gccgacgccg
     1441 gcgatgatgc ccttgacgac gaggctctcg atccacgtgc cgtcgggtaa gacgccggcg
     1501 gccagcgatc ccaaacccgc gaagcccaag tcgatcagat cgacgagcgg cgtggcccac
     1561 gcgaacacgg cttggaacac gaggaacatc agcacgatca gcgccgcggt acccggccac
     1621 ggacgattga tccagtcgat gaggcgggcg gtttgcgcga cgactccgga gcccggcgac
     1681 tcgacgcgcg ccagcgtctc gagaacccag gcgtagcggc ggcgcgcttc ggccgcgagc
     1741 ggcggctcga tgccgaacag cgccgtgcgt gcggccgcga tgtccgcccc ggcctgcgga
     1801 acgagcgtcg gcgcgctcgc gtcgatcaag agccgctcgg cctcgacgcg atgcaggtcg
     1861 gcgccgcggg cgcgccaatg agcgacgagc gcttcggccg cgcgctcgag ctcgggccaa
     1921 cgcgccgcct cgcgcggcgg cggcaccgcg agaatcgtcg cgagcgcggc gttgagcgcg
     1981 gggaaaccgc ggcccgaggt cgcgacgacg gggtacacgg gcacgccgcc gagcgcctct
     2041 tccagtgcct cgaagtcgat cctgcggcct tctcgctccg cggcgtcgat catcgtcagc
     2101 gccacgatca tcggccggcc gagcgccgtg agctgctgca gcaaatagag gccctgatag
     2161 agccgcgtcg agtcgatgac ggcgagtatc gcttcgggct tcggcgtgcc cgcgacgcgg
     2221 ccgaacagca cgtc
//
LOCUS       DASSNN010000066         3776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_15404, whole genome shotgun sequence.
ACCESSION   DASSNN010000066 DASSNN010000000
VERSION     DASSNN010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15404"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..2577)
                     /locus_tag="VFL84_01195"
     CDS             complement(61..2577)
                     /locus_tag="VFL84_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607265.1"
                     /translation="MGSWLADFRFDLRRGLKLLRKSPGFTAAAVLTLALGIGATSAIF
                     SVLHSIVLRPLPFPNPEQLVSVLEVNQRDGRIRPPTIEIQEAWRERSGAVLDVGVGVT
                     GGVDVSVTGPAGAARISLGGIALDTLSVLGVRPVVGRWFERDEVTVEGDTAATLVISY
                     GLWQEYFGGDPDVVGKSIPGWDAAWGRTIIGVMPPDFWVEPSMGDVKGWFAFNVGRFP
                     GARAPTLARLAPGVDYAQAEAELTTIALGIEAAGPNAADAADWRIRLEPLHDLVTAEY
                     EQTLYLLLGAVAFVLLISAVNVANLQLSRSVTRQSEMATRVALGAGRWRLIRQLLTEN
                     VLLALIGGALGIAVAYLGIWVFVTLAPGFYPPSQDIAIRPAVLLFTFGVSVSTGILAG
                     LFPALRISRPDLQDALKQSARGGGSGIRLGLRRALVVVEVAMALVLLVGAGLMINSYA
                     RVMRVDMGFEPDRLLTMEINLSGLSRYRTRRNASHFTVTPQVAELYREVMDRVQRLPG
                     VRSVGVTSGLPPRTSMTVPFRVVGQAGDPSDEFRNAQYYEVSADFFETMGIGLRRGRA
                     FTDADGENAAGVAIINETLARQYFGDADALGQIIEARVNRGNPNLADDRPREIVGIVA
                     DTRMRLRDDPRPMIYVPYRQHLTDYAGTGPFYVHARMDFAIRTELADPAAVTRAVRQI
                     VADVDPTVALDKVIPMRERLFDSAANDRFWLRLLGLFGGLAVFLATVGIYGVIAYAVE
                     QRAHEFSIRTAMGAGRRDIVLLVLREGIVVTVVGILIGIVAAFGLTRLIASQLYGVAP
                     TDPLTVAVVVLLLAFIAMLACVIPARHAANAEPLRALRVE"
     gene            2737..3603
                     /locus_tag="VFL84_01200"
     CDS             2737..3603
                     /locus_tag="VFL84_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7607266.1"
                     /translation="MKRALGNTGLAVGPIGLGGMPLSIQNRPDERTALAVIEAFIAAG
                     GDHIDTAISYCLDEHDFGHNERLIAKALRKVGRSDVVVATKGGLTRPDGRWEVDCSPQ
                     WLRHCCEQSAKTLGAPIPLYYLHAVDPAVPLAESLGELVRLRDEGNIAAIGLSNVNAR
                     QLDEALRMTPIAAVQNRCNVLDTRDFETGLVEHCRELGVAYVAYSPVGGHSGHRRLDQ
                     YPALARIADKHGVTPYVIALAWLLAQGPHIVPIPGATKISSVESSLSAPQVRLDAEDL
                     AALAALGSRKRH"
BASE COUNT          662 a         1323 c         1251 g          540 t
ORIGIN      
        1 ccagcgcaca taaccgggcc gtccaaacca gcctcttagc cgttacaacg atcgggcgcg
       61 ctattcgacg cgcagcgccc ggagcggctc cgcgttggcg gcatgacgtg ctgggatcac
      121 gcacgcgagc atcgcgatga aggccagcaa cagcacgacg acggcaaccg tgagcggatc
      181 ggtcggcgcg acaccgtaga gctggctcgc gatgagccgc gtcaagccaa acgccgcgac
      241 gatgccgatc agtatcccga cgacggtgac gacgatacct tcgcggagca cgagaagaac
      301 gatgtcgcgc cgcccggcgc ccatcgccgt gcggatgctg aattcgtgcg cgcgctgctc
      361 cacggcgtag gcgatgacgc cgtagatgcc gacggtcgcg agaaagaccg cgagcccacc
      421 gaagaggccg agcagccgca accagaatcg gtcgttcgcg gccgagtcga acaggcgctc
      481 gcgcatcgga atcactttgt cgagggcgac cgtcgggtcc acgtccgcca cgatctggcg
      541 aacggcccgc gtcacggccg cggggtcggc gagctcggtc cgtatcgcga agtccatgcg
      601 cgcgtgcaca tagaacggac ccgttccggc gtaatccgtc agatgctgcc ggtacggaac
      661 gtagatcatc ggcctcgggt cgtcgcgcag ccgcatccgc gtatcggcaa cgatgccgac
      721 gatctcgcgc ggccggtcgt cggcaagatt cggattaccg cgattgacgc gcgcctcgat
      781 gatctgaccc aacgcatcgg cgtcgccgaa gtactggcgc gccagcgtct cgttgatgat
      841 cgcgacgccg gcggcgtttt cgccgtccgc atcggtgaac gcgcggccgc gccgcaggcc
      901 gatccccatc gtctcgaaga agtcggcgct gacctcgtaa tactgcgcgt tgcggaactc
      961 gtcgctcgga tcgccggctt gcccgacgac acggaacggc accgtcatcg acgttcgcgg
     1021 cggcagcccg ctcgtgacgc cgactgagcg cacgccggga aggcgttgca cgcgatccat
     1081 gacctcgcgg tacagctcgg cgacttgcgg cgtgacggtg aagtgactcg cgttgcgccg
     1141 cgtccggtag cgcgagaggc cggacagatt gatttccatc gtgagcagcc ggtcgggttc
     1201 gaagcccatg tcgacgcgca tgacgcgcgc gtagctgttg atcatgaggc cggcaccgac
     1261 cagcaagacc aacgccatcg cgacctcgac gacgacgagc gctcgccgta accccaagcg
     1321 tatgccgctg ccgcctccgc gcgccgattg cttcagcgcg tcctgcaagt cgggacgcga
     1381 aatccgcagc gccgggaaca agccggccaa tattcccgtc gacaccgaca cgccgaacgt
     1441 gaagagcaac acggccggtc ggatcgcgat gtcttgcgac ggcggataga agccgggcgc
     1501 gagcgtcacg aacacccaaa tcccgagata cgcgacggcg atgccgagcg cgccgccaat
     1561 cagcgcgagc agcacgttct cggtcaagag ctggcggatc aggcgccagc ggccggcccc
     1621 gagcgcgacg cgcgtcgcca tttccgactg gcgcgtcacg cttctgctca gctgcaggtt
     1681 cgcgacgttg acggcggaaa tcagcagcac gaacgcgacc gcgccgagca gcaggtacag
     1741 cgtctgctcg tactcggcgg tgacgaggtc gtgcagcggc tcgaggcgga tgcgccagtc
     1801 cgcggcatcg gccgcattcg gcccggcggc ctcgatgccg agggcgatcg tcgtcagctc
     1861 cgcctccgct tgggcgtagt cgacgcccgg cgcaagccgc gccagcgtcg gcgcccgcgc
     1921 gcccgggaat cgcccgacgt tgaacgcgaa ccaccctttg acgtcgccca tggacggctc
     1981 gacccagaag tcgggcggca tgacaccgat gatggttctg ccccaggccg cgtcccatcc
     2041 cgggatggac ttgccgacga cgtcgggatc gccgccgaag tactcctgcc agaggccgta
     2101 gctgatgacg agcgtggcgg ccgtgtcgcc ctcgaccgtg acttcgtcgc gctcgaacca
     2161 gcggcccacg acgggccgca cacccagcac ggacagagta tcgagcgcga tcccgccgag
     2221 gctgatgcgc gcggcgccgg caggtcccgt gaccgagacg tcgacgcctc ccgtgacgcc
     2281 gacgccgacg tcgagcacgg cgccgctgcg ctcgcgccag gcttcttgaa tctcgatcgt
     2341 cggcggccgg atgcggccgt cgcgctggtt cacttcgagg accgagacca gctgctcggg
     2401 attcgggaac ggcagcggcc gcagcacgat cgagtgcagc acgctgaaga tcgcgctcgt
     2461 ggcgccgatg ccgagcgcca gagtcagcac ggccgcggcc gtgaagcccg ggctcttccg
     2521 cagcagcttc aaacctctgc gcagatcgaa gcggaagtcc gcgagccagg agcccaccta
     2581 accctccccg cgccgccaat ctcaatggcg cgccgtacct tagcaccgca tgccggtggt
     2641 gtccgcttcc tttgatgcgc gggtcgcgtc caaggttact cgcgagccgc tgcgcgggct
     2701 gacggcgcac gcgccgcacg ctgctacact cgtgcaatga agcgagcgct cggcaacacc
     2761 ggtctcgccg tcgggcccat cggcttgggc ggcatgccgc tctcgatcca gaaccggccc
     2821 gacgagcgca ccgcgctcgc cgtgatcgag gccttcatcg ccgccggcgg cgatcacatc
     2881 gacaccgcga tcagctactg cctcgacgag cacgacttcg gccacaacga acgcctcatt
     2941 gccaaggccc tgcgcaaagt tggccgaagc gacgtcgtcg tggcgacaaa aggcgggctc
     3001 acgagacccg acggccgctg ggaagtcgac tgcagcccgc agtggctgcg gcactgctgt
     3061 gagcagagcg cgaagacgct cggcgctccg atcccgttgt actacttgca cgcggtcgat
     3121 cccgccgtgc cgctcgcgga atcgctcggc gagctcgtgc ggctccgcga cgaaggcaat
     3181 atcgccgcga tcggtctatc gaacgtcaac gcgcgccagc tcgatgaagc cttgcgcatg
     3241 acgccgatcg ccgccgtgca gaaccgctgc aacgtgctcg acacgcgcga cttcgagact
     3301 ggcctcgtcg aacattgccg cgagctcggc gtcgcgtacg ttgcgtactc gcccgtcggc
     3361 gggcactcgg gtcaccggcg gctcgatcag tacccagcgc tcgcgcgcat cgccgacaag
     3421 cacggcgtga cgccgtacgt catcgcgctg gcgtggctgc tcgcgcaggg gccgcacatc
     3481 gtgccgattc cgggcgcgac gaagatctcg agcgtcgaga gcagcctctc cgcaccgcaa
     3541 gttcggctag atgcggagga tctcgcggcg ctcgccgcgc tcggctcgcg caagcggcac
     3601 tgattcgccg gaggggtcgg caaacatgca gaaaaagaag aacgcgcccc cgcgtcgggc
     3661 gctcagcttc gacgacgtgc gcgagatcgc gttgtcgatg cccgaagtcg aggagatcac
     3721 ggcgtacggc atgcccggat tcaaggccgg caagacccgc ttcgcggtgc aaccgt
//
LOCUS       DASSNN010000067         3093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_260456, whole genome shotgun sequence.
ACCESSION   DASSNN010000067 DASSNN010000000
VERSION     DASSNN010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260456"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFL84_01205"
     CDS             <1..241
                     /locus_tag="VFL84_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HET7607267.1"
                     /translation="VDGVVCGHIHRAELADVGGRLYCNDGDWVESCTALVEDQTGELS
                     LLEWRPTAATIAAAAVSVAAWAETIVSVDSEQEAA"
     gene            complement(280..843)
                     /locus_tag="VFL84_01210"
     CDS             complement(280..843)
                     /locus_tag="VFL84_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607268.1"
                     /translation="MDRLLLDRWTDTLAFRCLLLLSALAVLPVLAAGVLTTVIGGAAM
                     LYSRAAIELEQAVFGLLALGGMLGFIGYLRAHVAKRDPERLSVTTTLMFLAAGVVTAL
                     VVVGFAVAGVLDVWKAPWNARPLVVGSALFAAANLTWAIGGVAWMVRLPRLYAEDTGR
                     AFDGLPVVLMFVSLALAISAALAATTV"
     gene            complement(860..1216)
                     /locus_tag="VFL84_01215"
     CDS             complement(860..1216)
                     /locus_tag="VFL84_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607269.1"
                     /translation="MIIETTYLLYLAISVALTVWVARTLSKNGRVFLVNGFANDERLA
                     DSVNHLLVVGFYLINLGYVTLALKLGEKPVSTQEAIEFLSTKIGLVMLVLGAMHFFNV
                     FVVAKWGKRAAEAIRV"
     gene            complement(1288..1827)
                     /locus_tag="VFL84_01220"
     CDS             complement(1288..1827)
                     /locus_tag="VFL84_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7607270.1"
                     /translation="MNLTPVMTRFILHWGEMGTRWGVNRTVAQIHALLYLAPGPLPAD
                     EIAETLNVARSNVSTSLKELQGWGLVSIAHVMSDRRDHFTTRADPWDMLVTIMEQRKR
                     RELEPTLNFLRECARELEDDRVTPKEAKARIENMLGFLTSVTTWFDQMMALPRSTVMT
                     LMKLGTKIANFIPKTAKES"
     gene            complement(1950..2426)
                     /locus_tag="VFL84_01225"
     CDS             complement(1950..2426)
                     /locus_tag="VFL84_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HET7607271.1"
                     /translation="MKHATRWFCSLLLVLNGIAPIAFVSAQTADRAADEKAIRGVIQA
                     FLDTREKNDAAALAKLMTEDVDQQQTSGNVRRGRDAVVSGSLATQQSTGGKRTITLES
                     IRLIGSDVALADGRYDSVGRADGTDRHMLTSMVLRREGGAWKIAAIRNMLPSNPQR"
     gene            complement(2437..2988)
                     /locus_tag="VFL84_01230"
     CDS             complement(2437..2988)
                     /locus_tag="VFL84_01230"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HET7607272.1"
                     /translation="MNRMRRFSSSLVAMFALGLAGGAAAQAPAAASAPLPDGYMRAPA
                     VQKGLAPQMKVTESTGPAHVFDVAFTTGDEILSGLTDLAIKHNITSGYVTGLGGLSTA
                     LLGFGDPPLNAFRRIPVDEKSELVSLTGHIQTRDGVPVVHLHAIVALKDGSTKAGHVF
                     EAKVAPIAEISVVATSIGGAASH"
BASE COUNT          543 a         1043 c          992 g          515 t
ORIGIN      
        1 cgtcgacggc gtcgtctgcg gccacatcca ccgcgccgag ctcgccgacg tcggcggcag
       61 gctctattgc aacgacggcg actgggtcga gagctgcacg gcgctcgtcg aagaccagac
      121 cggcgagctg tcgctgctcg aatggcggcc gacggccgcg acgatcgcgg cggccgcggt
      181 atcggtcgcg gcttgggccg agacgatcgt cagcgtcgac tccgagcagg aagccgccta
      241 ggccttttga gatccgcccg gacccctcgg cggaggtgct caaactgtcg tcgccgcgag
      301 cgccgccgat atcgcgagcg caagcgagac gaacatcagc acgacgggca atccgtcgaa
      361 cgcgcggccg gtatcttcgg cgtagaggcg cggcaagcgc accatccaag ccacgccgcc
      421 tatcgcccac gtcaagttgg cggctgcgaa cagggccgag ccgaccacga gcggccgagc
      481 attccaagga gctttccaga cgtcgagcac gccggcgacc gcgaaaccga ccacgacgag
      541 cgccgtcacg acgccggccg cgagaaacat cagcgtcgtc gtcacactga gacgctcggg
      601 gtctctcttc gcgacgtgag cgcgcagata gccgatgaag ccgagcatcc cgcccagcgc
      661 gagcaggccg aagacggcct gctcgagctc gatggcagcg cgcgaataga gcatcgccgc
      721 gccgccgatg acggtcgtga gaacgcccgc cgcgagcacc ggcagcacgg ccagcgccga
      781 gagcagcagc aggcaacgaa atgccagcgt gtcggtccag cgatcgagca gcagtctgtc
      841 catggcaccc tcctcttcgc tacacgcgaa tcgcttcggc cgcgcgcttg ccccacttcg
      901 cgacgacgaa gacgttgaag aagtgcatcg cgccgaggac gagcatcacg aggccgatct
      961 tcgtgctcaa gaactcgatc gcttcctgcg tcgatacggg cttctcgccg agcttcagcg
     1021 cgagcgtcac gtaacccaag ttgatcagat agaaccccac gacgagcaag tgattgacgg
     1081 agtccgcgag tcgctcgtcg ttcgcgaagc cgttcacgag aaacacgcgg ccgttcttgc
     1141 tcagcgtgcg cgcgacccag accgtcaggg cgacgctgat ggcgagatac aggaggtagg
     1201 tcgtttcgat gatcatggcg ttctccttgc ttggcattac aggcatttct gtcgactcag
     1261 aaacacatgg gcaaaaaaac tccgcgttta gctttccttc gctgttttcg ggatgaagtt
     1321 cgcgatcttc gtgccgagct tcatcagcgt catgaccgtc gagcgcggca acgccatcat
     1381 ctggtcgaac cacgtcgtca cgctcgtcag gaagccgagc atgttctcga tgcgcgcctt
     1441 cgcctctttc ggcgtcacgc gatcgtcctc gagctcgcgc gcgcattcac gcaagaagtt
     1501 cagcgtcggc tcgagctcgc gccgtttgcg ctgctccatg atcgtcacga gcatgtccca
     1561 tggatcggct cgcgtcgtga aatggtcgcg gcggtcgctc atgacatgcg cgatgctgac
     1621 gaggccccag ccctgaagct ccttcaagct cgtgctgacg ttcgaccgcg ccacgttcag
     1681 cgtctcggcg atctcgtcgg ccggcagcgg acccggcgcg agatagagca gcgcgtggat
     1741 ctgcgcgacg gtgcgattga cgccccaccg ggtgcccatc tcgccccagt gcaggatgaa
     1801 tcgcgtcatg accggcgtta ggttcatttc ctgatatttc tgtcttttcc gaaataacga
     1861 taatccagtg gacggctggc gtcaagccct cgacccgccc acgaggtgct cgtcgcgtgc
     1921 ggccaaaagc acagggcggc ggccttaagt cagcgctgcg ggttgctcgg cagcatgttg
     1981 cgaatcgcgg cgatcttcca cgcgccgcct tcgcggcgca gcaccatcga cgtcagcatg
     2041 tgccgatccg tgccgtccgc tcggccgacg ctgtcgtagc ggccgtcggc gagcgcgacg
     2101 tccgagccga tgagtcggat cgactcgagc gtgatggtgc gcttgccgcc cgtgctctgt
     2161 tgcgtcgcca gcgagcccga gacgaccgcg tcgcggccgc gccggacgtt gcccgacgtc
     2221 tgttgctggt cgacgtcctc cgtcatgagc ttcgcgagcg cggccgcgtc gttcttctcg
     2281 cgcgtatcga gaaacgcctg gatcacgccg cggatggcct tctcgtcggc cgcgcggtcg
     2341 gccgtttgcg ccgacacgaa ggcgatcggc gcgatgccgt tcagtacgag gagcagcgag
     2401 cagaaccagc gggtggcgtg tttcatgtgg gaaccctcag tgcgacgcgg cgccgccaat
     2461 cgacgtcgcc acgaccgaga tctcggcaat cggcgccacc ttggcctcga acacgtgccc
     2521 ggcctttgtg ctgccgtcct tcagcgcgac gatcgcgtgc aggtgcacga ccggcacgcc
     2581 atcgcgcgtt tggatatggc ccgtcaacga gacgagctcg ctcttctcgt cgaccggaat
     2641 cctacggaac gcattgagcg gcggatcgcc gaagccgagc agcgccgtcg ataggccgcc
     2701 gaggcccgtg acgtatcccg acgtgatgtt gtgcttgatc gcaaggtcgg tgaggcccga
     2761 caaaatctcg tcgccggtcg tgaacgcgac gtcgaacacg tgcgcggggc cggtgctctc
     2821 cgtgaccttc atctgcggcg caaggccctt ctgcacggcg ggcgcgcgca tgtagccgtc
     2881 gggcaacggc gccgacgccg cggccggtgc ttgagccgcg gcgccgccgg cgaggcccaa
     2941 ggcaaacatg gcgacaaggg acgagctgaa acgacgcatg cggttcatgg tttcccccca
     3001 ttggccggcc tctccccaat ctaagcacga acccgcaacc agggggaatg gcccacgctg
     3061 cgccgcccgc cgctcgcctg actccacagg aag
//
LOCUS       DASSNN010000068         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1528994, whole genome shotgun sequence.
ACCESSION   DASSNN010000068 DASSNN010000000
VERSION     DASSNN010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528994"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VFL84_01235"
     CDS             complement(<1..838)
                     /locus_tag="VFL84_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HET7607273.1"
                     /translation="MARCLQQSGVIGKSKNDEQRAIADLARGIREQRVILFAGAGLSA
                     GLGLPSWQDFIGRLGTDLGFDAEAFLSLSPDFRSLAEYYCLEKGSLDGLRERMKREWA
                     VSDEKLRASAVYRHIVRLGFPLIYTTNYDDLLERAFELYGLPFNKVTTAKDIATRDQS
                     VPTIVKFHGDLAEGALVLTETDYFRRVAFEEPLDIQLMADALNRGILFLGYSASDVNL
                     RLLLFRLRQMWSARAEGGSRPTSYIVMTRPDRIQQRILDSWGVVPLIASSRESDDESA
                     ALM"
     gene            complement(849..2681)
                     /locus_tag="VFL84_01240"
     CDS             complement(849..2681)
                     /locus_tag="VFL84_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7607274.1"
                     /translation="MIRRFRRVSAAALAAWLLAFASSALAQAPVADAAMRRDTAEVRR
                     LLQSGADVKAAQADGATALHWAAYHGDASLASLLLDAGADVAAANRNGSTPMWLAASR
                     GDAAVVKALLAKGADANEPLPLGRRPLMLAARSGSVEAVRALLEKGADPNAKESARGT
                     TALMQAADQGHADVIEVLIEHGADFAAASAPVLRDARTAALGKSDDPRAAVRRQVIAI
                     LCDQPTPDLSVLQGLVAEQTEDQVLAQRSEPPKAPAGNPCSQDLQRDALGFVLAASGG
                     GRRGRAPREPDGGELTALVYAARSGSIEAARVLLDAGADVNQVTKYGWSPLLAATQNR
                     NYQLGKFLIEHGADVNLANKGGWTPLYLATDNRNLEGGDYPTRTPDMDHLEYIKLLLD
                     AGANVNAQITESTETRTVFTNQWLDEEGATAFLRASQSGDVELMKLLLERGADPKIYT
                     KLGVTPLAVAAGIGWVEGITYEHSPEQTVEAVKMLLDLGIDPNYQADTGRTALHGAAH
                     KGATEVVRVLVAAGARMDIRDFGNTDNRGSAELAAHTWLPIDYADGLVRVGVQSAIPH
                     PETAKVLRELMIAAGLDVPPEGRTLESICIVDVCSPEYNPIDYN"
BASE COUNT          407 a          924 c          970 g          463 t
ORIGIN      
        1 ccatgagcgc ggcggactcg tcgtcggact ctcgcgagct cgcgatcaac ggaacgacgc
       61 cccacgagtc gagtatgcgt tgctgaatcc ggtcgggccg cgtcatcacg atgtacgacg
      121 tcggccgcga tccgccctcg gcgcgagcgg accacatttg gcggagccgg aacagcagca
      181 gccgcaggtt cacgtcggat gcgctgtagc cgagaaacaa aattcctcgg ttgagcgcgt
      241 ccgccatgag ctggatgtcg agcggctcct cgaacgcgac gcggcggaag tagtcggttt
      301 cggtcagcac gagcgcgccc tcggcgaggt cgccgtggaa cttcacgatc gtgggaacgc
      361 tctggtcgcg agtcgcgatg tctttggcgg tcgtcacttt gttgaacggc aggccgtaca
      421 gctcgaacgc ccgctcgagc aggtcgtcgt agttcgtcgt gtagatcagc gggaaaccga
      481 ggcgaacgat gtgccgatag acggcggagg cccgcagctt ttcgtcggag acggcccatt
      541 cgcgcttcat gcgctcgcgc aagccgtcga gcgatccctt ctcgagacag taatactcgg
      601 cgagcgatcg aaaatcggga ctcaggctca agaacgcctc ggcgtcgaag ccgaggtcgg
      661 tgccgagacg gccgatgaag tcctgccagg agggcagccc gagcccggcc gataggccgg
      721 cgcccgcgaa caagatcacg cgctgctcgc gaatgccgcg cgcgagatcc gcgatggcac
      781 gctgctcgtc gttcttggac ttcccgatca cgcccgactg ctgcaagcag cgggccatgg
      841 cgccgagatc agttgtagtc gatcgggttg tattccggcg agcagacgtc gacgatgcag
      901 atcgactcga gcgtgcgtcc ctcgggcggg acgtcgagcc cggccgcgat catgagctcg
      961 cgcagcacct tcgccgtttc gggatgcggg atcgcggatt gcacgccgac gcgcacgagg
     1021 ccgtcggcgt agtcgatcgg cagccaggta tgcgcggcca gctcggcgct gccgcggttg
     1081 tcggtgttgc cgaagtcgcg gatgtccatg cgcgcgccgg ccgcgacgag cacgcgcacg
     1141 acctcggtgg cgcccttgtg cgccgcgccg tgcaacgccg tgcggcctgt gtcggcctga
     1201 tagttgggat cgatccctaa gtcgagcagc atcttcacgg cttccacggt ctgctcgggc
     1261 gagtgctcgt acgtgatgcc ctcgacccag ccgatgccgg ccgcgaccgc gagcggcgtc
     1321 acgccgagct tcgtgtagat cttcggatcg gcgccgcgct cgagcagcag cttcatgagc
     1381 tcgacgtcgc cggactgcga ggcgcgcagg aacgccgtcg cgccttcttc gtcgagccac
     1441 tgattcgtga agacggttcg cgtctcggtg ctctcggtga tctgggcatt cacattcgcg
     1501 cccgcgtcga gcagcagctt gatgtactcg aggtgatcca tgtcgggcgt gcgcgtcgga
     1561 tagtcgccgc cctcgagatt gcggttgtcg gtcgcgaggt agagcggcgt ccagccgcct
     1621 ttgttcgcga gattgacgtc ggcgccgtgc tcgatcagga acttgccgag ctgatagttg
     1681 cggttctgcg tcgccgcgag cagcgggctc cagccgtact tcgtcacttg gttcacgtcg
     1741 gcgccggcgt cgagcagcac acgcgcggct tcgatgctgc cggagcgagc cgcgtagacg
     1801 agcgccgtga gctcgccgcc gtcgggctcg cgcggcgcgc ggccgcgtct gccgccgccg
     1861 ctcgcggcga gcacgaagcc gagcgcatcg cgctgcaggt cttggctgca tggattgcct
     1921 gcgggagctt tgggcggctc cgaacgctgc gccagcacct ggtcctcggt ctgctcggca
     1981 acgagcccct gcagcacgct gagatccgga gtcggctggt cgcagagaat cgcgatgacc
     2041 tgccgccgca cggcggcccg aggatcgtcg gacttgccga gcgcggccgt gcgcgcgtcg
     2101 cgcaggaccg gagcggaggc ggccgcaaag tcggcgccat gctcgatcag cacttcgatc
     2161 acgtcggcat ggccttgatc ggcggcttgc atcagcgccg tcgtgccgcg cgcgctttct
     2221 ttcgcgttcg ggtccgcgcc cttctcgagc agcgctcgca cggcctcgac gctcccggag
     2281 cgcgcggcga gcatcagcgg cctgcgcccg agcggaagcg gctcgttggc gtccgcgcct
     2341 ttcgcgagca gcgccttgac gacggccgcg tcgccgcggc ttgccgcgag ccacatcggc
     2401 gtcgagccgt tgcggttggc cgccgcaacg tccgcgccgg cgtcgagcag cagcgaagcc
     2461 aagctcgcat cgccgtgata cgcggcccag tgcagcgccg tggcgccgtc cgcctgcgcg
     2521 gccttcacgt cggcaccgct ctgcaggagg cggcgcacct ccgcggtgtc gcgccgcatc
     2581 gcggcgtcgg cgaccggcgc ctgcgcgagc gcggagctcg cgaacgcgag cagccacgct
     2641 gcgagcgcgg ctgccgagac tctgcggaag cggcggatca tagggtttcg agccgtcccg
     2701 tgctgtcgcc gatgctgtct tggtgaatgt cgaactggtc gagcaggctc aacagcaaat
     2761 tgcc
//
LOCUS       DASSNN010000069         3781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_260629, whole genome shotgun sequence.
ACCESSION   DASSNN010000069 DASSNN010000000
VERSION     DASSNN010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260629"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1100)
                     /locus_tag="VFL84_01245"
     CDS             complement(30..1100)
                     /locus_tag="VFL84_01245"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HET7607275.1"
                     /translation="MTHPTDGQIELIDKPGLLTPSRSYKPLRYPWALDFWRRQQQIHW
                     MPEEIPLGEDCKDWSTRLTDAERNLLTQVFRFFTQSDVEVQDNYMERYARVFRPTEIK
                     MMLAAFANMETVHVAAYALLLETLGMSDSEFAAFLDYAAMRDKHDYLGTFGVDTEADI
                     LRTLAMFGGFAEGLQLFASFAILLNFPRHNKMRGMGQVVSWSVRDETLHCEGIIRLFH
                     TFASETGALTNAVRDDIVECARTVVRLEDRFIDLAFEQGPIEGLAAQEVKGYIRYVAD
                     WRLKQLALPALYGVKEHPLSWLTAQLNGVEHANFFETRATEYAKAATGGAWHGAEGAW
                     GLFDRRVSRRVQRELLGPTAHD"
     gene            complement(1097..2914)
                     /locus_tag="VFL84_01250"
     CDS             complement(1097..2914)
                     /locus_tag="VFL84_01250"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HET7607276.1"
                     /translation="MATQAAHVFDSQLTLDARRDARLTPFGRATLADRYLLPGETPQD
                     LFARVARTYADDGAHAQRLYDYMSCLWFMPATPILSNGGTDRGLPISCFLNAVEDDLA
                     SIVETWSENVWLASNGGGIGTYWGGVRSIGEPVRGCGHTSGIIPFVRVMDSLTLAISQ
                     GSLRRGSAAVYVDVHHPEIEEFIEIRKPSGDFNRKSLNLHHGVSITDEFMHAVRDDAP
                     FALRSPRTGEPLREVSARLLWQRLLETRLQTGEPYLLFIDTANRQLAEHQRAAGLVVR
                     QSNLCSEILLPTGRDHLGRNRTAVCCLASVNLERWDEWCGDDRFVEDVLRMLDNVLDD
                     FIERAPDSMAAARYSASRERSVGLGAMGFHSYLQLNGIPFESALARSANLRMFRHLRR
                     AADAASRRLAAERGACLDAAERGIAERFSHKLAIAPTASISVICGGTSACIEPIPANV
                     YTHKTLSGSFSVRNEALQKVLAAKGLDTEAVWQSILAHEGSVQHLEELSEHEKAVFRT
                     AFEIDQRWVVELAGDRAPLLCQGQSLNLYLRSDIHKWDLLMLHWMAWERGVKSLYYCR
                     SKSVQRAGFAGVDGDNTRAWRSRPLEANAEKYDECLACQ"
     regulatory      complement(3004..3212)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3254..>3781)
                     /locus_tag="VFL84_01255"
     CDS             complement(3254..>3781)
                     /locus_tag="VFL84_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7607277.1"
                     /translation="FSPAVAALLSKASYRKPDMVFDDELTLDLGGVHVRLSGVGPAHT
                     RGDTVMYVDEDKVLMAGDVVMPVFPAASAQAASIERWLASIAEFQRFAPRVVVPAHGR
                     LIDAATLARTREYLTAVQAQTRAAKREGQSVEAAQAAIAPKLAQQFGDLAPQGGGPAT
                     GRINAAIQAAYRESP"
BASE COUNT          616 a         1250 c         1270 g          645 t
ORIGIN      
        1 ttacggaacg actcggcctt cgataggcgt cagtcatgag ccgtcgggcc caatagctct
       61 ctctgaacgc ggcgcgacac gcgtcgatcg aacagccccc atgccccttc ggcgccatgc
      121 caggcgccgc ccgtcgcggc cttcgcgtac tccgtcgcgc gcgtctcgaa aaaattcgcg
      181 tgctcgacgc cgttcagctg cgccgtcagc catgacagcg ggtgctcttt gactccgtag
      241 agcgccggta acgccagctg cttgagccgc cagtcggcga cgtagcggat gtagcccttg
      301 acctcctgcg ccgcgaggcc ttcgatcggg ccctgctcga acgcgaggtc gatgaagcga
      361 tcctcgagcc gcacgacggt cctcgcgcac tcgacgatgt cgtcgcgcac ggcgttagtc
      421 agagcgccgg tctcgctcgc gaacgtatgg aacaggcgaa tgattccttc gcagtgcagc
      481 gtctcgtcgc gcacggacca cgagacgacc tggcccattc cgcgcatctt gttgtggcgc
      541 ggaaagttga gcaggatcgc gaagctcgcg aacagctgca ggccttcggc gaatccgccg
      601 aacatcgcga gcgtgcgcaa gatgtcggcc tcggtgtcga ctccgaacgt ccccaagtaa
      661 tcatgcttgt cgcgcatcgc ggcgtagtcg aggaaagccg cgaactcgct gtcggacatg
      721 ccgagcgtct cgagcagcaa cgcgtaggcg gcgacgtgca ccgtctccat gttcgcgaac
      781 gcggcgagca tcatcttgat ctcggtcgga cgaaacacgc gcgcgtagcg ctccatgtaa
      841 ttgtcctgga cctcgacgtc ggactgcgtg aagaagcgaa acacttgcgt gagcaggttg
      901 cgctcggcgt cggtgagccg cgtgctccaa tccttgcagt cctcgccgag cggtatttcc
      961 tcgggcatcc aatggatctg ctgctgccgc cgccaaaagt cgagcgccca cgggtagcgc
     1021 agcggcttgt agctgcggct cggcgtcagg aggcccggct tgtcgatgag ctcgatctgg
     1081 ccgtcggttg ggtgtgtcat tggcatgcga ggcactcgtc gtatttttca gcgttggctt
     1141 cgagcgggcg cgagcgccac gcgcgcgtgt tgtcgccgtc gacgccggca aacccggcgc
     1201 gctgcacgga tttcgagcgg cagtaataga ggctcttcac gccgcgttcc cacgccatcc
     1261 agtgcagcat caaaagatcc cacttgtgaa tgtcgctgcg caaatagaga ttcaacgact
     1321 ggccctgaca gagcagcggc gcccgatcgc cggcgagctc gacgacccaa cgctgatcga
     1381 tctcgaacgc cgtgcggaac acggccttct cgtgctcgct gagctcttcg aggtgctgga
     1441 cggagccttc gtgcgcgagg atcgactgcc agaccgcctc ggtgtcgagc cctttggccg
     1501 ccagcacctt ttgcagtgcc tcgttgcgca cgctgaacga gcccgacagc gtcttgtgcg
     1561 tgtagacgtt cgcgggaatg ggctcgatgc acgcgctcgt gccgccgcag atgacactga
     1621 tgctcgccgt cggcgcgatc gcgagcttgt ggctgaaccg ctcggcaatg cctcgctcgg
     1681 cggcgtccaa gcaggcgccg cgctcggcgg ccagacgcct ggacgcggca tccgcggcgc
     1741 gccgcaggtg gcggaacatg cgcaggttcg ccgagcgcgc gagcgcgctt tcgaacggga
     1801 tcccgttcaa ctgcaagtac gaatgaaagc ccatcgcgcc gagcccgacc gagcgctcgc
     1861 ggctcgcgga gtaacgcgcg gcggccatgc tgtcgggcgc gcgctcgatg aagtcgtcga
     1921 gcacgttgtc gagcatgcgc agcacgtcct cgacgaaacg gtcgtcgccg caccattcgt
     1981 cccagcgctc gaggttcacg gacgccaggc agcagactgc ggtgcggttc cggcccaagt
     2041 ggtcgcgtcc ggtcggcaac aggatctcgc tgcagagatt cgattggcgc acgacgagtc
     2101 cggctgcccg ttggtgctcc gcgagctgcc ggttcgcggt atcgatgaac agcaggtacg
     2161 gctcgccggt ctgcaagcgc gtctcgagca gccgctgcca gagcaagcgc gcagagacct
     2221 cacgcagcgg ctcgcccgtg cgcggactgc gcagcgcgaa cggcgcgtcg tcgcgaacgg
     2281 catgcatgaa ctcgtcggtg attgagacgc cgtggtgcag gttcaggctc ttgcggttga
     2341 agtcgcccga cggcttgcgg atctcgatga actcctcgat ctccgggtga tgcacgtcga
     2401 cgtagacggc cgccgagccg cgccgcagcg agccctgcga gatcgcgagc gtgagggagt
     2461 ccatgacgcg cacgaacggg atgatccccg acgtgtggcc gcagccgcga acgggctcgc
     2521 cgatcgagcg cacgccgccc cagtaagtgc cgatgccgcc gccgttcgac gcgagccaga
     2581 cgttctcgga ccaggtctcg acgatgctcg ccaaatcgtc ctcgacggcg ttcaagaagc
     2641 aggagatcgg caagccgcgg tccgtgccgc cgttgctcaa gatcggcgtg gccggcatga
     2701 accagaggca cgacatgtag tcgtagagcc gctgcgcgtg cgcgccgtcg tccgcatagg
     2761 tgcgtgcgac gcgcgcgaac aagtcttgcg gcgtttcacc gggcagcaag taccggtcgg
     2821 ccaacgtcgc gcggccgaac ggcgtcaacc gtgcgtcgcg ccgcgcgtcg agcgtgagtt
     2881 gcgaatcaaa gacgtgagcc gcctgcgtcg ccattctgcc ctcccgaagg cgctggggag
     2941 gtgggtaggg cgtggacgac cgactggccg tcgcgacgcg cctgtccagc acaccccggc
     3001 tgtcagtacg ttgcgcgccg cggcaggtct cctggctcgc cgctcgactg gagtcgtccg
     3061 gtcttcccga ggcggaaggc ctcagtgacg agaggggaca actccgttgc ggcttacagt
     3121 tgcgggggca gcttcggttt tgcggcgcaa gccgcgcacc gaattccctt ttcatcgcac
     3181 gacgagtgcg aaccgctgcg ccacaagata ttgggtccgc cagcgggatg ctgtcaacta
     3241 attgtagttt cggctagggc gactcgcggt aggccgcttg gatggcggcg ttgatgcggc
     3301 ccgtggccgg ccctccgcct tgcggcgcga gatcgccgaa ttgctgcgcc agcttcggcg
     3361 cgatcgccgc ttgcgcggct tcgaccgact gaccctcgcg ttttgcggcg cgcgtttggg
     3421 cttgcacggc ggtcaggtac tcgcgcgtgc gcgcgagcgt cgcggcgtcg atgagccggc
     3481 cgtgcgcggg caccacgacg cgcggcgcga accgttggaa ctccgcgatg ctggcgagcc
     3541 agcgctcgat gctcgcggct tgcgcgctcg cggccgggaa tacgggcatc acgacgtcgc
     3601 ccgccatgag aaccttgtcc tcgtcgacgt acatgaccgt atcgccgcgc gtgtgcgcgg
     3661 gcccgacgcc cgaaagacgc acgtgcacgc cgccgagatc gagcgtcagc tcgtcgtcga
     3721 acaccatgtc cggcttgcgg tagctcgctt tcgacagcaa ggccgcgacg gccggtgaaa
     3781 a
//
LOCUS       DASSNN010000070         3565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_16211, whole genome shotgun sequence.
ACCESSION   DASSNN010000070 DASSNN010000000
VERSION     DASSNN010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16211"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="VFL84_01260"
     CDS             complement(<1..1354)
                     /locus_tag="VFL84_01260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET7607278.1"
                     /translation="MTPTEPTQQRERSRSLVREVLGVQLAITAIGGLIAVAGLAWTSG
                     AVVRDNLEHWAAQWASELNELGAPFYLDDAGAAMLDVERFIAKYPEIERVTWYDTDGR
                     ALTSLDGDGTTFVDAKPLDAATAAELAARAGLYPPYLLTQNVSPGQRYRLSGPIWTES
                     FADDALFSSDPVAAATSVNRLGFVSVDLDFSGYEDALWPRLAMASGILLLLLGISWAC
                     GRWFLKSALSPLSELEHSLTELASETPNVRFPSSPYTELRAIVVALEDTTRALQKRER
                     RLLHLANHDQLTGLANRHRFVAELDTELARCADSGARSALFFVDLDQFKYVNDTCGHA
                     AGDHLLQLAAQQLRYAVRAEDFVARFGGDEFVVLVRNVSRSDARAAATKVLELMRGLK
                     HVEREQVFHLQCSIGVALLHGNRASAHEIIAQSDIACRSAKAHGRNRVEIYSAAGKQS
                     E"
     gene            complement(1341..2318)
                     /locus_tag="VFL84_01265"
     CDS             complement(1341..2318)
                     /locus_tag="VFL84_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607279.1"
                     /translation="MFLLLAGCAATGPATVGPDLEPIVVGAELLPVPPRPDPTPPPPA
                     PPREAPRADIALVVDPGAAAHADVADAIEAALPARRYRITRFTTAGQELEALRTRRVT
                     VVAVGPEAVIAARAALPDKPLVFCQVPAYTAALRPGTPTWGVETLPPLSLQLKSWQSL
                     DPSLRTIALIVGASGAALAEQARHAATELAADLLVEKSGSDRETLYIFRRIAPTVDGL
                     WLLPDNDALSPQVLLEMLTLSSTRGIGVLTFNEALLTRGALLTATAVPSDVAETVARI
                     VERVVAGRTADLPAMTPLRAAELAVNVDVAAALGLPPVTVPRWVARDPD"
     gene            complement(2438..2932)
                     /locus_tag="VFL84_01270"
     CDS             complement(2438..2932)
                     /locus_tag="VFL84_01270"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HET7607280.1"
                     /translation="MLDSLMDWLISTPINGLVLNYAWSWPTLESLHFLALCTLMGSLL
                     IMDLRLIGMNRLIPLQAVHSLMPVAIVSFAINLITGLGFIFGDPHTYFANWAFWAKMI
                     LILAAGANFLLYYTAVEPKLLRLGPAEATPPLAKAVGALSLVFWFGVLSFGRLLPYLG
                     TGGG"
     gene            complement(2945..3406)
                     /locus_tag="VFL84_01275"
     CDS             complement(2945..3406)
                     /locus_tag="VFL84_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607281.1"
                     /translation="MGFLTWLEATGLAEWVRSSLPGYPSMIALHALGMAIMVGLSLLL
                     DLRILGWFGGIPLQALRRFFGIAWIGFGINFISGSMLFSAQATSYIVDWVFMTKMTLV
                     LLGAITAGMLQPAVAKVAPGAQLSGGTKAIAGVAIVIWLVAIVMGRLTAYL"
BASE COUNT          614 a         1171 c         1277 g          503 t
ORIGIN      
        1 gctcgctctg cttgccggcg gcgctgtaga tctcgacgcg gtttcggcca tgcgccttcg
       61 cggaacggca agcaatgtcg ctttgggcaa tgatttcgtg cgcgctggcg cggttgccgt
      121 gcaacagcgc gacgccgatg ctgcactgca gatggaacac ctgctcgcgc tcgacgtgct
      181 tgaggccgcg catgagctcg agcactttcg tcgcggccgc gcgcgcgtcg ctgcgcgaca
      241 cgttgcgaac gagcacgacg aactcgtcgc cgccgaagcg cgcgacgaag tcctcggcgc
      301 gcaccgcgta gcgcagctgt tgcgcggcga gctggagcaa atggtcgccg gcggcgtggc
      361 cgcacgtgtc gttgacgtac ttgaactggt cgaggtcgac gaagaatagc gcgctgcgcg
      421 cgccggagtc ggcgcagcgc gcgagctccg tgtcgagctc ggcgacgaag cgatggcggt
      481 tcgcgagacc cgtcagctgg tcgtggttgg cgaggtgcag cagccggcgc tcgcgctttt
      541 gcagcgcgcg agtcgtgtcc tcgagcgcta ccacgatcgc gcgcagctct gtgtacggcg
      601 agctcgggaa ccggacgttc ggcgtctccg acgcgagctc ggtgagcgag tgctcgagct
      661 cggacagcgg cgacagcgcg ctcttcagga accagcggcc gcacgcccac gagatgccga
      721 gcagcaggag caggatgccg cttgccatcg cgaggcgcgg ccagagcgca tcctcgtaac
      781 cggagaagtc gaggtcgacg ctgacgaacc cgagccggtt gacggacgtc gccgcggcca
      841 cggggtcgga gctgaacaac gcgtcgtcgg cgaatgactc ggtccagatg gggccggaca
      901 ggcggtaacg ctgcccgggt gagacgttct gcgtgagcag gtacggcgga tagaggcccg
      961 cccgggccgc aagctcggct gcggtcgcgg cgtcgagcgg ctttgcgtcg acgaacgtcg
     1021 tgccgtcgcc gtcgagcgag gtcagcgcgc gcccgtccgt gtcgtaccag gtgacgcgct
     1081 cgatctcggg gtacttggcg atgaagcgct cgacgtcgag catcgcggcg cccgcgtcgt
     1141 ccaagtagaa cggcgcgccg agctcgttca gctccgacgc ccactgcgcg gcccagtgct
     1201 cgaggttgtc gcggacgacc gcgcccgagg tccacgcgag cccggcgacg gcgatcaatc
     1261 cgccgatcgc ggtgatggcg agctgtacgc cgagcacctc gcggacgaga ctgcggctgc
     1321 gctcgcgctg ttgcgtcggc tcagtcgggg tcacgggcca cccagcgcgg tacggtgacg
     1381 ggcggcaagc cgagagccgc cgcgacgtcg acgttgacgg cgagctccgc ggcgcgtaac
     1441 ggcgtcatcg cgggaaggtc ggccgtgcgg ccggcgacga cgcgctcgac gatccgggcc
     1501 acggtctcgg cgacgtcgga aggaaccgcc gtggccgtca gcaatgcgcc gcgcgtgagc
     1561 agcgcttcgt tgaacgtcag cacgccgatg ccgcgcgtcg acgacagcgt cagcatctcg
     1621 agcaagactt gcgggctcag cgcgtcgttg tcgggcagca gccagagccc gtcgacggtc
     1681 ggggcgatgc gccggaaaat atagagcgtc tcgcgatcag accccgactt ttcgacgagc
     1741 aagtcggcgg cgagctcggt cgcggcatgg cgcgcctgtt ccgcgagcgc cgcgccggac
     1801 gcaccgacga tcaacgcgat cgtgcgcagg gacggatcca gggactgcca gctcttgagc
     1861 tgcagcgaga gcggcggcag cgtctcgacg ccccaggtcg gcgtgcccgg ccgcagcgcc
     1921 gcggtgtagg ccgggacttg gcagaagacg agcggtttgt caggcagcgc cgcgcgcgcg
     1981 gcgatcacgg cttcggggcc cactgcaacg accgtgacgc gccgggttcg aagggcctcg
     2041 agctcttgcc ccgccgtcgt gaagcgagtg atgcgatagc ggcgcgcggg cagggcggct
     2101 tcgatcgcgt cggcgacgtc ggcatgcgcc gccgcgcccg ggtccaccac gagcgcgata
     2161 tcggccctgg gcgcttcgcg cggcggcgca ggcggcggcg gcgtggggtc gggccgcggg
     2221 ggcaccggca gcagctctgc accgacgacg ataggctcga ggtccggccc aacggtagcg
     2281 ggccccgtgg ccgcacagcc ggcgagcagc aggaacatga ccgccgccgg cggcccggtt
     2341 ggtcggtagg cgctccgcat ggaactgcca gcctacgggt ccggcgcccc gggccggcgt
     2401 gctggccttc acagtccgcg cggcgcgcgg agagacgtca gccgccgcct gttcccaaat
     2461 acggcagcaa tcggccgaac gacagcacgc cgaaccagaa cacgagcgac agcgcgccga
     2521 ccgccttcgc gagcggcggc gtagcctcgg cagggccgag cctcaggagc ttcggctcga
     2581 cggccgtgta gtacaggagg aagttcgcgc cggcggcgag gatcaggatc atcttggccc
     2641 agaacgccca attcgcgaag tacgtgtgcg ggtcgccgaa tatgaagccc aagccggtga
     2701 tcaagttgat cgcgaacgac acgatcgcga cgggcatgag gctatgcacg gcttgcagcg
     2761 ggatcagccg attcatgccg atcaggcgca ggtccatgat cagcagcgag cccatgagcg
     2821 tgcacaacgc gaggaagtgc agcgactcga gcgtcggcca gctccacgcg tagttcagaa
     2881 cgaggccgtt gatcggcgtc gagatcagcc agtccattag cgagtcgagc atgttctgag
     2941 ctccttacag gtacgccgtc agccggccca tgacgatcgc gacgagccag atgacgatcg
     3001 caactcccgc gatggctttc gtgccgcccg acaattgtgc gccgggcgcg accttcgcga
     3061 cagccggttg cagcatcccg gccgtgatcg cgccgagcag gacgagcgtc atcttcgtca
     3121 tgaagaccca gtcgacgatg tagctcgtcg cttgcgccga gaacagcatc gagcccgaga
     3181 tgaagttgat gccgaagccg atccacgcga tgccgaagaa acgccgcagc gcttgcagcg
     3241 ggatgccgcc gaaccagccg agaatgcgca aatcgagcag cagagacagg ccgaccatga
     3301 ttgccatgcc gagggcatgc agtgcgatca tcgacgggta gccggggagc gacgatcgca
     3361 cccactcggc aagcccggtg gcctcgagcc aagtcaagaa tcccacggaa aagcccccta
     3421 acgtgacgtc gtagaaatta ttctggcggc agcgtagccg caggcagtgc ggctgtccac
     3481 ttaagaaacc caatgcgacg cgtgcgcgtc gcattgttgc caacctcgcg gaccgtcacc
     3541 tatagtcggc cgccgaatga ccgtc
//
LOCUS       DASSNN010000071         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_16212, whole genome shotgun sequence.
ACCESSION   DASSNN010000071 DASSNN010000000
VERSION     DASSNN010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16212"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFL84_01280"
     CDS             complement(<1..361)
                     /locus_tag="VFL84_01280"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HET7607282.1"
                     /translation="MDAFIDWIYGTRLSWFVTAYPWVWPILESLHFCALTVLAGTVGL
                     FDLRVLGLVKGITPGALHATLRWGVAAFAVSAITGVMFVAGASEQYFYNDAFRLKVVF
                     LVLMGANAALFYWREFAL"
     gene            complement(361..867)
                     /locus_tag="VFL84_01285"
     CDS             complement(361..867)
                     /locus_tag="VFL84_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607283.1"
                     /translation="MMAFFANVEQTGLSLWVRESPSLWAFPFILFLHTLGLAMLAGLN
                     VGLDAWLLGRRKLPPFELANIYRVMWLGFGINALSGLVLLAAYPAKALTNWVFFAKLM
                     FIAIAVWVLQRLKGELAVAGGTVERDVTRRARHLALASLVLWTATIFAGRLLAYTHHV
                     LLVSEGLS"
     gene            complement(864..>2011)
                     /locus_tag="VFL84_01290"
     CDS             complement(864..>2011)
                     /locus_tag="VFL84_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7607284.1"
                     /translation="VIEPPEPEVPQPAVDERALDAAVALLAAAKRPMIMCGAGAQHAP
                     REVALLAETLQAPVTAFRSGRGVVPEDHALGVASVAARELWDDVDVLVGVGSRLEMPY
                     MRWGDSMRYERKPSHGPKLIRIDIDPEEMRRFEPDVGVVADSATACRLLAERLRGRVA
                     PSRTRVAEIAAAKRVAEAAIERIQPQTAFLRAIRAALPRDGLLVPELSQVGFTTYTGA
                     FPVLAPRTYISEGFQGTLGFGFPTALGVKVANPSKAVVAVTGDGGFMFGVQELATAAQ
                     YGIALVTIVFNNNSFANVLRDQQLQFGGRTLGSRLHNPDFVRLAESFGVAARRVKEPH
                     ELRRVLEAELAAGRPALIEVPLEAGVEQSPWPLIHMRKRPSELGAHR"
BASE COUNT          373 a          683 c          679 g          276 t
ORIGIN      
        1 tcagcgcaaa ctcgcgccag tagaacaacg cggcgttcgc gcccatgagc acgagaaaca
       61 cgaccttgag cctgaacgcg tcgttgtaga agtactgctc ggatgcgccc gcgacgaaca
      121 tcacgccggt aatcgcgctg accgcgaaag ccgcgacgcc ccaccgcaac gtcgcgtgca
      181 aggcgcccgg cgtgatgcct ttgacgagcc cgagcactcg caagtcgaac aggcccacgg
      241 tgccggcgag aaccgtcagg gcgcagaaat gcaggctttc cagaatcggc cacacccaag
      301 ggtacgcggt gacgaaccag gaaagccgcg tgccgtagat ccaatcgatg aaagcgtcca
      361 tcacgacagg ccttcgctga cgagcaagac gtggtgcgtg taggcgagca gccggccggc
      421 gaagatcgtt gcagtccaca gcacgagcga cgcgagtgcg aggtgccgcg cgcggcgcgt
      481 gacgtcgcgt tcgaccgtcc cgcccgccac ggcgagctct cccttcaagc gctgcagcac
      541 ccagactgcg atcgcgatga acatgagctt cgcgaagaac acccaattgg tcagcgcttt
      601 ggccggatag gcggcgagca gcacgagccc cgagagcgcg ttgataccga agccgagcca
      661 catcacgcga tagatgttag cgagctcgaa cggcggcagc ttgcggcgcc cgaggagcca
      721 ggcgtcgagg ccgacgttca ggcccgcgag cattgcgagc cccagcgtgt gcaggaacag
      781 gatgaacgga aaggcccaca gcgacggcga ttcgcgcacc cagaggctca gccccgtctg
      841 ctccacgttc gcgaagaacg ccatcatcga tgcgcgccga gctcgctggg acgcttgcgc
      901 atgtgaatca ggggccatgg cgactgctcg acgccggcct cgagcggcac ctcgatcagc
      961 gccgggcgcc cggccgcgag ctcggcctcg agcacgcgcc gcagctcgtg cggctctttg
     1021 acgcgccgcg cggcgacgcc gaaactctcg gcgagccgca cgaaatccgg attgtgcaag
     1081 cgcgagccga gcgttcgacc gccgaattgc agttgctggt cgcgcagaac gttggcgaac
     1141 gagttgttgt tgaagacgat cgtcacgaga gcgattccgt attgcgccgc cgtcgcaagc
     1201 tcctgcactc cgaacatgaa gccgccatcg ccggtcacgg ccacgaccgc tttgctcgga
     1261 ttcgcgacct tgacgccgag agccgtcgga aaaccgaagc cgagtgtgcc ctggaaaccc
     1321 tcggagatat aggtccgcgg cgccaagaca ggaaacgcgc ccgtgtacgt cgtgaagccg
     1381 acttgcgaga gctcgggcac gagcaggccg tcgcgcggta gagcggcgcg gatcgcgcgc
     1441 aggaacgccg tctgcggttg aatgcgctcg atcgcggcct cggcgacgcg ctttgccgcc
     1501 gcgatctcgg cgacgcgtgt gcggctcggc gcgacgcggc cgcgcagccg ctccgcgagc
     1561 aatcggcacg cagtcgccga gtcggcgacg acgcctacat cgggctcgaa ccgccgcatt
     1621 tcctcgggat cgatgtcgat gcggatcagc ttgggcccat gcgacggctt gcgctcgtag
     1681 cgcatcgaat cgccccagcg catgtacggc atctcgaggc ggctgccgac gccaacgagc
     1741 acgtcgacgt cgtcccagag ctctcgagcc gcgacgctgg cgacgccgag cgcgtgatct
     1801 tcgggcacga cgccgcggcc gctgcgaaac gccgtcacgg gcgcctgcag cgtctcggcg
     1861 agcagggcga cttcgcgcgg cgcatgctgc gcgcccgcgc cgcacatgat catcgggcgc
     1921 ttggctgccg cgagcagcgc gacggccgca tcgagcgcgc gttcgtcgac ggcgggctgg
     1981 ggcacttcgg gctcgggcgg ctcgatcacg a
//
LOCUS       DASSNN010000072         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1402689, whole genome shotgun sequence.
ACCESSION   DASSNN010000072 DASSNN010000000
VERSION     DASSNN010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402689"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VFL84_01295"
     CDS             <1..808
                     /locus_tag="VFL84_01295"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7607285.1"
                     /translation="SVTLDGVVPPPLALGPDVAREAQRALEQIFDRCANDSACAARFA
                     SLPQLFAEVLARLDAGAADETDPPPISALELRALVRFMSYSAATVALLPVLISEAHAG
                     NYAPLEGQARTLLRELPESLSFPMSNSVACTEDLPYADGATSDLAETYLGTGVMDALK
                     QICARWPVGTIDASFKTPVVSDTPVLLLSGEYDPITPPSYAERVKAEGLGNSVHLVGR
                     GQGHGLVGVGCVPRLMRAFLEDPAPAKLDAACLALEPPTPFFLTLLGPAP"
     gene            805..1539
                     /locus_tag="VFL84_01300"
     CDS             805..1539
                     /locus_tag="VFL84_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7607286.1"
                     /translation="MIEIDAISKSFGAVRALDRVGFEARDGRITGLLGPNGAGKSTCL
                     RILSTVLEADGGTARVGGIDLKTSPLAARRVLGVLPHSSGLYQQLTGRENIEYYGRLH
                     GLREDALAERVDALIERLELGLIANRKAKGFSQGERTKIALARALVHDPQHLLLDEPT
                     SGLDVMATRHLREWLRELRGQGRCVLLSSHVMQEVEALVDELVIIAAGRVTLTGSPAE
                     LRQRFPGLSLEEIFVEAVRGTERSAA"
     gene            1536..>2681
                     /locus_tag="VFL84_01305"
     CDS             1536..>2681
                     /locus_tag="VFL84_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607287.1"
                     /translation="MSALFTVCRKEIIETLRERRTLFSLLLGPLFGPILFTVLINVMV
                     SRNLSSQDEVLDVPTLGAEHAPNLIAFLRARSIEPVAKHDIADVEDAAALVATGAYDV
                     VLVIDDKFDEDFGTDRAARVALVFDRSKNRAASRVSRVRGAVEGYGQQIGALRLVANG
                     VDPSLLRPLVVDELDVSTPASRSALMLGVLTYFLLFATLTGGLHLAIDTTAGERERKS
                     LEPLLTLLVPRSSLIVGKMTATVCYMLASLALTLVAFTIALKQMPLEQIGMSSTFGFT
                     TAAVAFLLLAPFAPLGAALMTTVASFTKTYREAQTYLTFVLLIPTLPLAFATILNIEP
                     APKLMWIPSLSQHLLVTTLIKDQPLAPAFVALSAASTLLFGVLLGLLA"
BASE COUNT          373 a          943 c          931 g          434 t
ORIGIN      
        1 ctcggtgacg ctcgatggcg tcgtgccgcc gccgctcgcg ctggggcccg acgttgcgcg
       61 cgaagcgcag cgcgcgctcg agcagatttt tgatcgctgc gccaacgact cggcctgcgc
      121 ggcacggttc gcgtcgctgc cgcagctctt cgccgaggtg ctcgcgcggc tcgacgccgg
      181 cgcggccgac gagaccgatc cgccgccgat cagcgcgctc gagctgcgcg cgctcgtgcg
      241 gttcatgagc tacagcgccg cgaccgtcgc gctgctgccc gtgctgatca gcgaggcgca
      301 cgccggcaac tacgcgccgc tcgagggcca ggcgcgcacg ctgctgcgcg agctgcccga
      361 atcgttgagc tttccgatga gcaactccgt ggcctgcacg gaggacttgc cgtacgccga
      421 cggcgcgacg agcgatctcg cagagaccta tctcggcacg ggcgtaatgg acgcgctgaa
      481 gcagatctgc gcgcgctggc cggtcggcac gatcgacgct tcgttcaaga cgcccgtcgt
      541 cagcgacacg cccgtgctgc tcctgtccgg cgaatacgac ccgatcacgc cgccgtcgta
      601 cgcggagcgc gtcaaagccg aagggctcgg caacagcgtg cacctcgtcg gccgcggcca
      661 aggccacggc ctcgtcggcg tcggctgcgt gccgcgcctg atgcgcgctt tcctcgagga
      721 ccctgctccc gcgaagctgg acgccgcgtg tcttgcgctc gagccgccga cgccgttttt
      781 tctgacgctg ctcgggccgg cgccgtgatc gagatcgacg ccatcagcaa gagcttcggc
      841 gccgtgcgcg cgctcgatcg cgtcggattc gaagcgcgcg acgggcgcat cacgggcttg
      901 cttgggccga acggcgccgg caagtcgacg tgcctgcgaa tcctgtcgac ggtgctcgag
      961 gccgatggcg gcacggcgcg cgtcggcggc atcgacttga agacgtcgcc gctcgcggcg
     1021 cgccgcgtgc tcggcgtgct gccgcacagc tcgggcctct atcagcagct cacgggccgc
     1081 gagaacatcg agtactacgg ccgcctgcac gggttgcgcg aggacgcgct cgccgagcgc
     1141 gtcgacgcgc tgatcgagcg gctcgagctt gggctcatcg cgaaccgcaa ggcgaaaggc
     1201 ttctcgcagg gcgagcgcac gaagatcgcg ctcgcgcgcg cgctcgtcca cgacccgcag
     1261 cacctgctgc tcgacgagcc gacgagcggc ctcgacgtca tggcgacgcg gcacctgcgc
     1321 gaatggctgc gcgagctgcg cggccagggc cgctgcgtgc tgctgtcgag ccacgtcatg
     1381 caggaggtcg aagcgctcgt cgacgagctc gtcatcatcg cggcgggccg cgtcacgctg
     1441 acgggcagcc cggccgagct caggcagcgc ttcccgggct tgagcctcga ggagattttc
     1501 gtggaagccg tgcgcggcac cgagcggagc gccgcatgag cgcgctcttc acggtgtgcc
     1561 gcaaggagat catcgagacg ctgcgcgagc ggcgcacgct gttctcgctg ctgctcgggc
     1621 cgctgttcgg gccgatcctg ttcacggtgc tgatcaacgt catggtctcg cgcaatcttt
     1681 cgtcgcagga cgaggtgctc gacgtgccga cgttgggcgc agagcacgcg ccgaatctga
     1741 tcgcgttctt gcgggcgcgc agcatcgagc ccgtcgcgaa gcacgacatc gcggacgtgg
     1801 aggacgccgc ggcgctcgtc gcgacgggcg cgtacgacgt cgtgctcgtc atcgacgaca
     1861 agttcgacga ggacttcggc acggatcgcg cggcacgcgt cgcgctcgtc ttcgaccgct
     1921 cgaagaatcg cgcggccagc cgagtcagcc gcgtgcgcgg cgccgtcgaa ggctacgggc
     1981 agcaaatcgg cgcgctgcgg ctcgtcgcga acggcgtgga cccgagcttg cttcggccgc
     2041 tcgtcgtcga cgagctcgac gtctcgacgc cggcgagccg ctcggcgctg atgctcggcg
     2101 tgctgacgta tttcctgctg ttcgcgacgc tgacgggcgg gctgcatctc gcgatcgaca
     2161 cgacggccgg cgagcgcgag cgcaagtcgc tcgagccgct gctgacgctg ctggtcccgc
     2221 ggtcgagcct gatcgtcggc aagatgacgg cgacggtctg ctacatgctg gcgtcgctcg
     2281 cgttgacgct cgtcgcgttc acgatcgcgc tgaagcagat gccgctcgag cagatcggca
     2341 tgagctcgac gttcggcttc acgacggccg ccgtcgcgtt cctgctgctc gccccgttcg
     2401 cgccgctcgg cgccgcgctg atgacgacgg tggcgtcgtt cacgaagacc tatcgcgaag
     2461 cgcagactta cctcacgttc gtgctgctga tcccgacgct gccgctggcg ttcgcgacga
     2521 tcctcaacat cgagcccgcg ccgaagctga tgtggatccc gagcttgagc cagcacctgc
     2581 tcgtgacgac gctgatcaag gatcagccgc tggcgccggc gttcgtcgcg ctctcggccg
     2641 cgagcacgct tctcttcggc gttcttctcg gcctcctcgc c
//
LOCUS       DASSNN010000073         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_16430, whole genome shotgun sequence.
ACCESSION   DASSNN010000073 DASSNN010000000
VERSION     DASSNN010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16430"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VFL84_01310"
     CDS             complement(<1..663)
                     /locus_tag="VFL84_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607288.1"
                     /translation="MGLTTAEPIATQLATKAIGGLAAALFGSLLAGLCAGVGAFGARM
                     AAPLTRIGRWPAPLAAIAAGMFVVGLQFALASLALPDAPRWPGSAWGSQALPVAGGLL
                     SGAGFIAFASLELFVVYVVARLTRGFSQRLWLAVAVVVVLECAAALVQGRSNLAGALV
                     SGLIAGAAASSVLLLLLRYDPRLVPAFAATVVLMTGAVKAAQAGAWLPFAVDAATTVI
                     VAA"
     gene            653..>2007
                     /locus_tag="VFL84_01315"
     CDS             653..>2007
                     /locus_tag="VFL84_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607289.1"
                     /translation="MRPIATEGHALRDDSTAMTNEAQDADDERGKARSEEQDDCDEAQ
                     DLQPVVALALGTRPRFRHVHEAAHSRDDIAGDGDGVARFAARTDDDAWCAEHERPVGA
                     RRALLLVGRDERELVRIDFERRRERPARERQRRLTRQARTGARLGNRVRERAHAPIVG
                     DRDTPFLCALDDVAVEFRIAHFPHRRREDRPHEAAVGVGTRLAPHELVPCPLPGLLAR
                     ELHGREALPFGRQRHAARGERRVGGGDGVRPGKPQRRNVRAERRKHGPRTDARDTQHR
                     QRALERARDPARGERVGDRAIVGVRAGAPRAIAQYVGQFRPAPGLPAAQDDDRERDER
                     GDHDERLLRACARRVDQEERNRKEREVERRVQQDRGQQAEAQEDQRPHDRGRQQLDRP
                     RIRREPRIVGVRAAEYERLQDHGERDAQRAMAVPCADQRAERKRYRAEDAFLHESRLQ
                     R"
BASE COUNT          358 a          731 c          718 g          200 t
ORIGIN      
        1 ggcggcgacg atcaccgtgg tcgccgcgtc gacggcgaac ggcagccacg cgccggcctg
       61 cgcagctttg accgcgccgg tcatcagcac gacggtcgcg gcgaacgcag gcacgagccg
      121 cgggtcgtag cgcaacagca gcagcagcac ggagctggca gcggcgccgg cgatcaatcc
      181 ggacacgagc gcgcccgcga ggttgctgcg accctgcacg agcgccgccg cgcattcgag
      241 cacgacgacg acggcaaccg cgagccacag ccgctgcgaa aagccgcgcg tcaggcgggc
      301 caccacgtag acgacgaaca gctcgaggct cgcgaacgcg atgaagccgg cgccggacag
      361 caatccgccg gcgacgggca gcgcctgcga accccacgcg gagcccggcc aacgcggggc
      421 gtcgggcaat gcgagcgagg cgagcgcgaa ctgcagtccg acgacgaaca tgccggccgc
      481 gatcgccgca agcggcgccg gccagcgtcc gatgcgcgtg agcggcgcgg ccatgcgcgc
      541 gccgaacgcg cccacgcctg cgcagagtcc cgcgagcaac gagccgaaga gcgccgctgc
      601 aaggccgccg atcgccttgg tcgcaagctg cgtcgcgatc ggctccgcag tcgtgagacc
      661 catcgcgacc gaaggccacg cattgaggga cgacagcacc gcgatgacga acgaagccca
      721 ggatgccgac gacgagcgcg gcaaggcccg cagcgaagaa caggatgact gcgacgaggc
      781 ccaggatctc cagccggtgg ttgcgctcgc gctcggaacg cgtccacgtt tccggcacgt
      841 gcacgaagcg gcccattccc gcgacgacat cgccggcgac ggtgacggcg tagcgcgctt
      901 cgccgcccgc accgacgacg atgcttggtg cgccgaacac gaacgaccag tcggtgcgcg
      961 ccgggcgctg ctcctcgtcg gccgcgacga gcgtgagctt gtccgcatcg acttcgaacg
     1021 acgtcgcgag cgcccggctc gcgagcgcca gcgccgcctc acgcgacagg cgcggaccgg
     1081 cgcgcgtctc gggaaccgcg tgcgagaacg cgcgcatgcg ccgatcgttg gtgatcgcga
     1141 cacgccattc ctctgcgcgc tcgacgacgt cgccgtcgaa tttcgcatag cgcacttccc
     1201 acatcggcgg cgcgaggatc gtccccacga ggcggcggta ggcgtcggga cccgcctcgc
     1261 gccacacgaa ctggtgccat gtccattgcc cgggctcctc gctcgcgagc ttcacggtcg
     1321 cgaagcgctg ccattcggcc gacagcgtca cgccgcgcgc ggcgagcgcc gcgtcggcgg
     1381 cggcgatggc gtccgcccgg gaaagccgca gcggcggaac gtccgcgctg aaaggcgcaa
     1441 gcatggccca cgcaccgatg cccgcgacac ccagcaccgg cagcgcgcgc tggaacgcgc
     1501 gcgagacccg gcccgcggcg agcgcgtcgg ggaccgcgcg atcgtcggtg tgcgcgccgg
     1561 ggcgccacgc gccattgcgc agtacgtcgg gcaattccgc ccagcgcccg gcctgccagc
     1621 ggcgcaggac gacgaccgcg agcgggacga gcgcggcgat cacgacgagc gcctgttgcg
     1681 cgcgtgcgcc cggcgcgtcg atcaggaaga gcggaatcga aaagagcgcg aggtcgaacg
     1741 tcgcgtgcag caggatcgtg ggcaacaggc cgaagcgcag gaagatcagc gcccacacga
     1801 ccgagggcgc cagcagctcg accggccgcg aatacgccgg gaaccccgga tagttggcgt
     1861 gcgcgccgcc gaatacgagc gcctgcagga tcacggcgag cgcgatgccc agcgtgcgat
     1921 ggccgtaccg tgcgccgatc agcgcgccga gcgaaagcgg tatcgcgcgg aagacgcatt
     1981 cctccatgaa tcccgcctgc agcgaca
//
LOCUS       DASSNN010000074         5884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1024906, whole genome shotgun sequence.
ACCESSION   DASSNN010000074 DASSNN010000000
VERSION     DASSNN010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024906"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="VFL84_01320"
     CDS             <1..1305
                     /locus_tag="VFL84_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7607290.1"
                     /translation="RAFLIFSDWDERKHTADEIMQPVLAEARKLPGVRVSMGRPGGLG
                     RGGLQSPIVAVIGGPDYEHLAMWSDQMLQLAQANPGLANVQSNYKERKPQIRVSIDRD
                     RAADLGISLESVGRTLETVLGSRIVTTYIDRGREYNVILQGRDDIRETTTDLTNIRVR
                     SARTDELIPLSSVVRLEETSGTMQLSRFDRLRAIKISSDLAPGYTMGEAVKWFEDAVA
                     RELPEATLDWDGQTREFTRTGQQLYFTFLLALAVVYLVLAAQFESFVHPIIIMVTVPL
                     ALLGAVFGLKLFGLTVNIFSQIAVIMLIGIAAKNGVLIVEFANQLRDRGLGFVDAVVQ
                     AAVTRLRPVLMTSLCTAFGALPLMLASGAGAEQRRPIGIVVFFGMTVAVFLTLFAVPT
                     VYGLLARKTKSPQHVSRLVDRLMGGGGAGSAGEAVVHDVHRE"
     gene            complement(1311..1694)
                     /locus_tag="VFL84_01325"
     CDS             complement(1311..1694)
                     /locus_tag="VFL84_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET7607291.1"
                     /translation="MRVAIDTNRYVDLCKGVAETVEVLETAEVVVLPFVVLAELRAGF
                     LLGRRQVENERALHGLLLKDGVEVLYADDQTTHHYASVYRQLRKQATPIPTNDMWVAA
                     LVLQHNLALHARDRHFEHLPQLVRI"
     gene            complement(1691..1933)
                     /locus_tag="VFL84_01330"
     CDS             complement(1691..1933)
                     /locus_tag="VFL84_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607292.1"
                     /translation="MQYTIRRIPKAVDDAVRERARVTGKSLNEAAVDALADGAGLKGI
                     PRKRRDLSDIAGTWKADKAFEAALADQDRIDKDLWR"
     gene            complement(2053..2484)
                     /locus_tag="VFL84_01335"
     CDS             complement(2053..2484)
                     /locus_tag="VFL84_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607293.1"
                     /translation="MQTRPQGWLYLAACAVLVAGCASTSENDNRDARNARLPTGVTLL
                     DSNREECQGSVAIDDSLIAGARREDLVIQRGGNATFELDAKSDDDIEIDWTCVGSANT
                     QRERIECPEGTSWLRVTRATGDDFLLECYGERGSTTRRARR"
     gene            complement(2562..3236)
                     /locus_tag="VFL84_01340"
     CDS             complement(2562..3236)
                     /locus_tag="VFL84_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607294.1"
                     /translation="MSFQDRSLRLAAVFAVGLGLASAAFAGPPLICHPFTTGAAAPLL
                     PWAEGNDHWHKPDPGYDRAHLVADTLRLLSADAAILDRMENMRRATIYAEEDPSMAAE
                     LLRAVVERTKTKPASARAEALEWFDAGYLVETYRQLGLIYEHEMLPGHGRWTSLVPVE
                     LTELDGYALVQKAAALAPEFEAELDFASALLSREPLTAAHLRQAASRAAQGSLLAQNL
                     AHYDVP"
     gene            3410..4342
                     /locus_tag="VFL84_01345"
     CDS             3410..4342
                     /locus_tag="VFL84_01345"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607295.1"
                     /translation="MSPSGDDVRRQLDRLLASAVFANAGRMSRFLKFVVERTLAGEGE
                     RLKEYVIGVEVFDRDASYDPRLDSIVRVEAARLRSKLAEYYAGEGRADAVVLSLPKGG
                     YAPIVTLEQRPVAASGAPPTNGTSALPPGQTTEAARRTQARWRNWTPAVLLVAGVAIA
                     AWIWIPDLLPVGPVGPLRIAVLPFTPDGDDADTAALAQRITEGVTAELVRAGRFGVVA
                     SSAARAEYAVTRRPRDVAAALNADAVVEARLSVDGPLVRVEARAAAGDTEEKLWVQSF
                     AADVADTDALEREIASALSAAFSYAKLVDETGSQ"
     gene            complement(4336..5484)
                     /locus_tag="VFL84_01350"
     CDS             complement(4336..5484)
                     /locus_tag="VFL84_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HET7607296.1"
                     /translation="MRRSTEKILTTHVGALPGPLETWQGGAGDAALRSAVRDVVAAQL
                     AAGVDVVNEGELTKGGNWVTFINSRLSGFAAVPAEGTAALLRSSADWKEFDDFYSKAL
                     AGGTLFEQTASVPTQTSGIRDWVCREPIRYTGQQALAREIELLKSTLGAHAVGDAFLT
                     STAPASIEVGRRNEHYRSDEEFLEALAEALRVEYEAIANAGLLVQIDDAWLPALWDRI
                     GIRMGLPAYRRYCMVRVEALNHALRNVPEEQVRYHLCWGSWHGPHKHDIPLADLVDVM
                     LAVKAQAYLFEAANARHEHEHTLWQSVKLPAGKILAPGVVTHATTIVEHPELVSQRIQ
                     RFARLVGRENVVASTDCGLGLRCHAQIAWAKLAALSEGAALASKALRY"
     gene            complement(5501..>5884)
                     /locus_tag="VFL84_01355"
     CDS             complement(5501..>5884)
                     /locus_tag="VFL84_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607297.1"
                     /translation="ANNMPTAIKNGWFDGTVSGSWNYAMEKTVDAIVAERDEKDAAKA
                     ERLWGDGLMALHRTVTNSDKGYRLHSAYKLCTWMRGLIPSPFLRAPQRRLPVEQVVEL
                     RDALKAAHYEVISDAAMRKAYPGLS"
BASE COUNT          960 a         1998 c         1931 g          995 t
ORIGIN      
        1 cgcgcctttc tgatcttctc cgactgggac gagcgcaagc acaccgccga cgagatcatg
       61 cagcccgtgc tcgccgaggc gcgcaagctg cccggcgtgc gcgtctcgat gggccggccc
      121 ggcggcctag gccgcggcgg ccttcaatcg ccgatcgtcg ccgtcatcgg cggaccggac
      181 tacgagcacc tcgctatgtg gtccgatcag atgctgcagc tcgcgcaagc gaacccaggc
      241 ctagcgaacg tacagagcaa ctacaaggag cgcaagccgc agatacgcgt ctcgatcgac
      301 cgcgaccggg cggccgatct cggcatctcg ctcgagagcg tcggccggac gctcgagacc
      361 gtgctcggct cgcgcatcgt cacgacgtac atcgatcgcg gccgcgagta caacgtcatt
      421 ctgcagggac gcgacgacat ccgcgagacg acgacggacc tcacgaacat ccgcgtgcgc
      481 tccgcgcgca cagacgagct gatcccgctg tcgagcgtcg tgcggctcga ggagacgtcg
      541 ggaacgatgc agctctcgcg gttcgatcgc ctgcgcgcga tcaagatctc gtccgacctc
      601 gcgccgggtt acacgatggg cgaggccgtg aagtggttcg aggacgccgt cgctcgcgag
      661 ttgcccgagg cgacgctcga ctgggacggc cagacgcgcg agttcacgcg caccggccag
      721 cagctctact tcacgttcct gctcgcgctc gccgtcgtct atctcgtgct cgcggcgcag
      781 ttcgagagct tcgtgcatcc gatcatcatc atggtcaccg tgccgctcgc gctgctcggc
      841 gccgtgttcg gtctgaagct gttcgggctg accgtcaaca tcttcagcca gatcgccgtg
      901 atcatgctga tcggaatcgc ggccaagaac ggcgtgctga tcgtggaatt cgcgaatcag
      961 ctgcgcgacc gcgggctcgg attcgtcgac gccgtcgtgc aagcggccgt gacgcgactg
     1021 cgtcccgtgc tcatgacgag cctgtgcacg gcgttcggcg cgttgccgct catgctcgcg
     1081 agcggcgccg gcgccgagca gcggcggccg atcggtatcg tcgtgttctt cgggatgacg
     1141 gtcgcggtgt tcctgacgct gttcgccgtg ccgaccgtct acgggctatt ggcacggaag
     1201 acgaagtcgc cgcagcacgt cagccgcctc gtcgaccggc tcatgggcgg cggcggcgcc
     1261 ggcagcgccg gcgaagccgt cgtccacgac gtgcaccgcg aatagcaagg tcagatcctt
     1321 actagctgcg gtaggtgctc gaagtgccga tcacgggcgt gcaacgctag attgtgttgc
     1381 agcacgagcg ccgccaccca catgtcgttc gtcggaatgg gcgttgcctg cttgcgcaat
     1441 tggcgataga ccgacgcgta atgatgcgtc gtttggtcgt ccgcatagag aacctcgaca
     1501 ccgtccttca gcaacaagcc gtgcaaggcg cgctcgtttt cgacttgccg acgaccaaga
     1561 aggaaccctg cgcgcagctc tgcaagtacc acgaacggca gaaccaccac ctcggccgtt
     1621 tcgagcacct cgactgtttc ggcgactccc ttgcaaagat cgacgtaacg attcgtgtcg
     1681 atcgcgactc tcatcgccac aaatccttgt cgatgcgatc ttgatcggcg agcgccgctt
     1741 cgaaggcctt atcggctttc cacgtaccgg caatgtcgct gagatcacgc cgcttccgtg
     1801 gaatgccctt cagccccgcg ccgtcggcca aagcgtcgac ggccgcttcg ttgaggctct
     1861 ttccagtgac ccgcgcgcgc tcgcgcacgg cgtcgtcaac cgcttttgga atgcgacgaa
     1921 tggtgtactg catgcaccaa gtataggcgg tgcatgcact atcagcaagc taacgcaagt
     1981 agggcgtaga cgagagctaa cggaaagcgg gcagcccgcg tcgtgcacgg gctacccacc
     2041 ccattggacg gatcagcgcc gcgcgcgtct cgtggtcgag ccgcgctcgc cgtagcactc
     2101 gagcaagaag tcgtcgcccg tggcgcgcgt gacacgcagc cacgacgtgc cctcggggca
     2161 ctcgatcctc tcgcgctgcg tgtttgcgct gccgacgcaa gtccagtcga tttcgatatc
     2221 gtcatcggac ttcgcgtcga gctcgaacgt cgcgttccct ccgcgctgga tcacgagatc
     2281 ctcgcgccgc gcgcccgcaa tgaggctgtc gtcgatcgcc accgagccct ggcactcctc
     2341 gcgattggaa tcgagcagcg tgacgccggt cggcagccgc gcgttgcgtg cgtcccggtt
     2401 gtcgttctcc gacgtcgatg cgcagccggc taccagcacc gcgcaagccg ccaaatagag
     2461 ccaaccctgc ggtcgtgttt gcatgtcctc tctccttgag gttttttcgc gtactcaaga
     2521 agcaacatcc atgccaaaca aaccgcagga tcggtaacgt ttcaagggac gtcgtagtga
     2581 gcgagatttt gcgcgagcaa cgatccctgc gccgctctcg aggccgcctg gcgcagatgc
     2641 gccgccgtca acggctcgcg cgacaacaag gccgacgcaa aatcgagctc ggcttcgaat
     2701 tccggcgcga gcgcggcggc cttctgcacg agcgcataac cgtcgagctc cgtgagctcg
     2761 acgggcacga gcgacgtcca gcgtccgtgg cccggcagca tttcatgctc gtagatcagc
     2821 ccgagctgac gatacgtctc gacgaggtag cccgcgtcga accattcgag cgcttcggcg
     2881 cgcgcgctgg ccggcttcgt cttcgttcgt tcgacgacgg cgcgcagcag ctcggccgcc
     2941 atgctcggat cctcctcggc gtagatcgtg gcgcggcgca tgttctccat gcggtcgagg
     3001 atcgccgcat cggccgagag caagcgcagc gtatcggcga cgagatgagc gcggtcgtaa
     3061 ccagggtcgg gcttgtgcca gtggtcgttg ccttcggccc acggcagcaa cggcgccgcg
     3121 gcgccggtcg tgaacgggtg acagatcagc ggcgggcccg cgaacgccgc ggacgcgagc
     3181 ccgagcccta cggcgaacac tgcggcgagg cgcaacgagc ggtcttggaa cgacatgggg
     3241 gtctcctcaa ggttgcggga aagtccgcga gattcagtgt cgtcggccga ttctaagcgg
     3301 cgggagtcag tcgccgttag gttctaaccg ttagtaaccc cacgcgggcc cgtggccgcg
     3361 cccgttttgg cgacggccca ggccgcgcag ttgtgggaca ctacgcgcca tgtcaccgag
     3421 cggagacgac gttcgccgcc agctcgaccg gctgctcgcg agcgccgtgt tcgcgaacgc
     3481 cggacgcatg agccggttct tgaagttcgt cgtcgagcga acgctcgccg gcgaaggcga
     3541 gcgcctcaag gaatacgtga tcggcgtcga ggttttcgac cgcgacgcga gctacgaccc
     3601 gcgcctcgac tcgatcgttc gcgtcgaggc ggcccggttg cgctcgaagc tcgccgagta
     3661 ttacgccggt gaaggccgcg ccgatgccgt ggtcttgagc ctgccgaagg gcggttatgc
     3721 gcccatcgtg acgctggaac aacggccagt tgccgcgagc ggcgcaccgc ccacgaacgg
     3781 cacatccgcg cttccgccgg gtcagacaac ggaagcggca cgtcggacgc aggcgagatg
     3841 gcggaattgg acgcccgccg tcttgctcgt cgcgggcgtc gcaatagcgg catggatatg
     3901 gattcccgat cttctgccgg tagggcccgt cggacccctt cggatcgctg tgctgccctt
     3961 cacaccggac ggcgacgatg cggacaccgc cgcgctcgcg cagcgcatca ccgagggtgt
     4021 aactgcagag ctcgttcgcg ccgggcgctt cggggtagtc gcgagctcag cggcgcgcgc
     4081 cgagtacgca gtaacccgca ggccgcgcga cgtcgccgca gcgctaaacg cggatgccgt
     4141 cgtcgaagcg cgactctcgg tggacgggcc gctcgttcgt gtcgaagcgc gcgccgccgc
     4201 cggcgacacc gaagaaaaac tttgggtcca gagctttgcc gctgacgtcg cagacaccga
     4261 cgcgctcgag cgggaaatcg cgtctgcgct ctccgccgcg ttcagctacg ccaagctcgt
     4321 agacgagacc ggaagtcagt agcgcaaagc cttgctcgcg agcgcggcgc cttccgacaa
     4381 cgcggccagc ttcgcccatg cgatctgggc atggcagcgc aagccgagcc cgcagtccgt
     4441 cgacgcgacg acgttctcgc ggccgacgag gcgcgcgaag cgttggatgc gctgcgacac
     4501 gagctccgga tgctcgacga tcgtcgtcgc gtgcgtcacg acaccgggcg ccaagatctt
     4561 gcccgccggc agcttcacgc tctgccacag cgtgtgctcg tgctcgtggc gcgcattcgc
     4621 ggcctcgaac aggtacgcct gcgccttcac ggcgagcatg acgtcaacca agtcggcgag
     4681 cggaatgtca tgcttgtgcg ggccgtgcca gcttccccag cagaggtggt accgcacctg
     4741 ctcctcgggc acgttgcgaa gcgcgtggtt caaggcttcg acgcgcacca tgcagtagcg
     4801 ccggtacgcg ggcaggccca tccggatgcc gatgcgatcc cagagcgccg gcagccacgc
     4861 gtcgtcgatc tgcacgagca ggccggcgtt cgcgatcgct tcgtactcga cgcgcagcgc
     4921 ttccgcgagc gcttcgagga attcctcgtc gctccggtag tgctcgttgc ggcgcccgac
     4981 ctcgatgctc gccggcgccg tcgacgtcag gaacgcgtcg ccgacggcgt gcgcgccgag
     5041 cgtcgacttc aagagctcga tctcgcgcgc gagcgcttgc tgacccgtgt agcggatcgg
     5101 ctcgcggcag acccagtcgc ggatcccgga ggtctgcgtc ggcacgctcg cggtttgctc
     5161 gaacagcgtg ccgccggcga gcgccttcga gtagaagtcg tcgaactcct tccagtccgc
     5221 gctcgagcgc agcagcgccg cggtgccttc ggcgggcacg gcggcgaagc ccgagaggcg
     5281 cgaattgatg aaggtgaccc agttgccgcc cttcgtgagc tcgccctcgt tgacgacgtc
     5341 gacgccggcc gcgagctgcg cggcgacgac gtcgcggaca gccgaccgca gcgccgcgtc
     5401 gccggcgccg ccctgccagg tctcgagcgg gcccggcagc gccccgacgt gagtcgtcag
     5461 gatcttctcg gtgctacggc gcaagcaacg tggctggcgg tcagctcaat ccaggatatg
     5521 ccttgcgcat cgcggcgtcg gagatgacct cgtagtgcgc ggccttcaac gcgtcgcgca
     5581 gctcgacgac ctgctcgacg ggcagccggc gctgcggcgc gcgcaggaac gggctcggaa
     5641 tcaggccgcg catccacgtg cagagcttgt acgcgctgtg gagccgataa cccttgtcgg
     5701 agttcgtgac cgtgcgatgc agcgccatca agccgtcgcc ccacaacctc tcggccttcg
     5761 ccgcgtcctt ctcgtcgcgc tcggctacga tcgcgtcgac ggtcttctcc atcgcgtagt
     5821 tccacgagcc gctgacggtg ccgtcgaacc agccgttctt gatcgcggtc ggcatgttgt
     5881 tggc
//
LOCUS       DASSNN010000075         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1773325, whole genome shotgun sequence.
ACCESSION   DASSNN010000075 DASSNN010000000
VERSION     DASSNN010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773325"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VFL84_01360"
     CDS             <1..1108
                     /locus_tag="VFL84_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HET7607298.1"
                     /translation="IGRKQARLRVDDVGFSVGPVGDHARAAEVLLDRLLPGSGASAGR
                     LVAIGHRIVHGGTQFTGPVRITTDVELELDALRDLAPLHNPPALAVIRATATRYSRVP
                     LYAVFDTSFFFTLPAHAREYALPPEWRYGRGIRRFGFHGLAHEYLARQSRRSPALTRR
                     LVTLQLGYGCSAAALLDGKPVDTSMGFSPLEGLIMATRPGDLDAGVLLELERRGEAWS
                     TIAHVLNREAGLKGLSGATGDVRELLELEAHGHAGAALAMAAFHHRILKYLGAYTAIL
                     GGLDAIAIGGGIGENAPQVRAQICRGLEWLGVELDAEANDNAVGVAARITKASSSVTV
                     DVIPVDEELLIAERVRDALPIAAHADEGIARSAS"
     gene            1105..>2339
                     /locus_tag="VFL84_01365"
     CDS             1105..>2339
                     /locus_tag="VFL84_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HET7607299.1"
                     /translation="MSGKLADDDLGALDAYWRAANYLSVGQIYLLSNPLLEEPLTLEH
                     VKPRLLGHWGTTPGLNFIYAHLNRVIKARELPMLLVIGPGHGGPALVANTWLEGTYSE
                     LYPDVSRDAEGMRRLFKQFSFPGGIPSHCAPEIPGSIHEGGELGYSLAHAFGAAFDNP
                     QLVVACVIGDGEAETGALAASWHSRVFLHPVDDGAVLPILHLNGWKIANPTVLARMPE
                     EELRELLRGYGWDPRFVSGDEPMRMHRAMASALDAAVDEIARIKAAARGEASKGDVPR
                     WPMLVLRTPKGWTGPHEVDGVPVEGTWRSHQVPLASLAENPAHLRALEQWLRSYRAEE
                     LFTAEGAPRPELLAHVPAGTLRLGATPYANGGVLRRNLRLPDYRDYAVAVPSPGTVRA
                     EATRVLGAYLRDVFVKNEKS"
BASE COUNT          344 a          827 c          817 g          351 t
ORIGIN      
        1 catcggccgt aagcaggcgc ggttacgcgt cgacgacgtc ggattctccg taggcccggt
       61 cggcgatcac gcacgggccg cggaggtgct gctcgatcgg ctgctgccgg gctcgggcgc
      121 atcggccggc aggctcgtcg cgatcgggca ccgcatcgtg cacggcggca cgcagttcac
      181 ggggccggtc cgcatcacga ccgacgtcga gcttgagctc gatgcgctgc gcgacctcgc
      241 gccgctgcac aacccgccgg ctctggcggt catccgcgcc acggcgacgc gctactcgcg
      301 ggttcccctc tacgcggtat tcgatacgtc gttcttcttc actctgccgg cgcacgcgcg
      361 cgagtacgcg ctgccgcccg aatggcgcta cgggcgcggc attcgccgct tcggcttcca
      421 cgggctcgcg cacgagtacc tggcccgcca gtcgcggcgc tcgccggcgc tgacgcggcg
      481 gctcgtgacg ctgcagctcg gctacggctg ctcggccgcg gccttgctcg acggcaagcc
      541 cgtcgacacg agcatgggat tctcgccgct cgaaggtctg atcatggcga cgcgcccggg
      601 cgatctcgac gcgggcgtgc tgctcgagct cgagcgtcgc ggcgaggcgt ggtcgacgat
      661 cgcccacgtg ctcaatcgtg aagcggggct caagggcctc tccggcgcga ccggcgacgt
      721 gcgcgagctg ctcgagctcg aagcgcacgg ccacgcgggg gcggcgctcg cgatggcggc
      781 gttccatcac cgcatcttga agtacctcgg cgcgtacacg gcgatcctcg gcgggctcga
      841 cgccatcgcg atcggcggcg gcatcggcga gaacgcgccg caagtgcgcg cgcagatctg
      901 ccgcgggctc gagtggctcg gtgtcgagct cgacgccgag gccaacgaca acgccgtcgg
      961 ggtcgcggcc cgcatcacga aggcgagctc gtccgtcacg gtcgacgtca ttccggtcga
     1021 cgaggaactg ttgatcgccg agcgcgtccg cgacgcgctt ccgatcgccg cacacgcgga
     1081 cgaaggcatc gcgcggagcg cgtcgtgagc ggaaagctcg ccgacgacga cctcggcgcg
     1141 ctcgacgcgt actggcgcgc cgcgaactac ctctccgtcg ggcaaatcta tctgctctcg
     1201 aacccgctgc tcgaggagcc gctcacgctc gagcacgtga agccgcgcct gctcggccat
     1261 tggggcacga caccggggct caatttcatc tacgcgcatt tgaatcgcgt catcaaagcg
     1321 cgcgagctgc caatgctgct cgtgatcggg cccggccacg gcggcccggc gctcgtcgcg
     1381 aacacgtggc tcgagggcac gtactcggag ctgtatcccg acgtctcgcg tgatgccgaa
     1441 ggcatgcggc ggctgttcaa gcagttttcg ttcccgggcg gcattccgag ccactgcgcg
     1501 cccgagatac cgggatcgat ccatgaaggc ggcgagctcg gttactcgct cgcacatgcg
     1561 ttcggcgccg cgttcgacaa tccgcagctc gtcgtcgcgt gcgtcatcgg cgacggcgag
     1621 gccgagaccg gtgcgctcgc cgcgagctgg cactcgaggg tgttcctgca ccccgtcgac
     1681 gacggcgccg tgctaccgat cctgcatttg aacggctgga aaatcgcgaa cccgaccgtg
     1741 ctcgcccgca tgcccgaaga ggagctccgc gagctcctgc gcggctatgg ctgggatccg
     1801 cgcttcgtga gcggagacga gccgatgcga atgcaccgcg cgatggcgag cgcgctcgat
     1861 gcggccgtcg atgaaatagc gcggatcaaa gccgcggcac gcggcgaggc gtcaaagggc
     1921 gacgtgccac gctggccgat gctcgtgctg cgtacgccga agggctggac cggaccgcac
     1981 gaggtcgacg gcgtccccgt cgaaggcacc tggcgctcgc atcaggtgcc gctcgcgtct
     2041 ctcgccgaga atccggcgca tctgcgcgcg ctcgagcaat ggctacgcag ctatcgcgcc
     2101 gaagagctct tcacggcaga aggcgcgccg aggcccgagc tgctcgcgca cgtgccggcg
     2161 ggcacgctgc ggctcggcgc gacgccgtat gcgaacggcg gcgtgctgcg ccgcaacctg
     2221 cggctgcccg actaccgcga ctacgcggtg gccgtgccga gccccggcac cgtgcgcgcc
     2281 gaagccacgc gcgtgctcgg ggcttacctg cgcgacgtgt tcgtcaagaa cgagaagtc
//
LOCUS       DASSNN010000076         4956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1152463, whole genome shotgun sequence.
ACCESSION   DASSNN010000076 DASSNN010000000
VERSION     DASSNN010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152463"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFL84_01370"
     CDS             complement(<1..724)
                     /locus_tag="VFL84_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7607300.1"
                     /translation="MSAASRRDCDVLIAGGGAVGSALACALGELPLDVVLVEALKPQL
                     LAQPSFDARVTALANGSQQILSGLGLWRELAGYVESIRSIHISERGRFGAARIDAREE
                     HVPALGYTVENQTLGRVLWERLEHTRRLTVLAPAKVVALKQEPGHAEVTVERGGAAEI
                     VRAKLVVAADGARSAVRAALGVATSEDDYAQRAVVFNCSTEARLDGRAFERFSDRGPI
                     ALLPLTGGRGAVIWTLPGDDAGG"
     gene            complement(721..2028)
                     /locus_tag="VFL84_01375"
     CDS             complement(721..2028)
                     /locus_tag="VFL84_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HET7607301.1"
                     /translation="MNAKEFARRRRHLMELIGANGIAVLAAAPERVRSRDTHYVYRPD
                     SDFFYLTGFREPEAVAVLVPGRPQGEFLLFCRERNPEREQWDGPRAGPEGAVAQFGAD
                     DAFPISDVDDILPGLLERSDRVYYSIGGNEFDQKLLSFIQTLNSKRQIGHAPSELVSL
                     DHLLHEMRLFKSRSETGVMRQAARIAAGAHGRAMRACRAGMKEYELEAEFVHEFRRHG
                     AECSYPPIVAAGANACILHYRNNDAPLVDGELVLIDAGCELDMYASDVTRTFPVNGRF
                     SAPQRELYEIVLDANRAATAKAKAGNHWNDPHDAAVGVVTRGLKELGLLDGRVPQLVK
                     SLAYRQFFMHKTGHWLGLDVHDVGDYKVADQWRLLEPGMVMTIEPGVYVSANDKSVDK
                     RWRGIGIRIEDDVQVTRLGPEVLTEGVPTTVAEIERHMNGRGA"
     gene            complement(2048..2617)
                     /locus_tag="VFL84_01380"
     CDS             complement(2048..2617)
                     /locus_tag="VFL84_01380"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HET7607302.1"
                     /translation="MTPSSATPPLDYERLRDSLAASGAVVALAELHGGVCGALCAGGS
                     VAARRWLTDSLDDVELGASAASLAEDLEELIGTSSKMLEDDELTFEPLLPSDEAPLPE
                     QVEALAVWCQGFLSGVGSTAPAAEGDAFREVLHDFAEISRAGLSDEEAAGEGQPDFAL
                     AQIHEYVRVSVQLVFEELAPVRAAARDLH"
     gene            2611..2943
                     /locus_tag="VFL84_01385"
     CDS             2611..2943
                     /locus_tag="VFL84_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HET7607303.1"
                     /translation="MSRERSAQCARAEGNASTRVLLTLDQRGSILPRMTPDQANHRVD
                     LELKRLEKRLEELVVVCRQLQEENRSLRHRQDSLMSERATLLQKNEQVRGRVEAMIGR
                     LKGMEQVS"
     gene            2940..3260
                     /locus_tag="VFL84_01390"
     CDS             2940..3260
                     /locus_tag="VFL84_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET7607304.1"
                     /translation="MSGTGTFAHVTIKILEKEYNVACPIEEKPQLLASAELLNAKMRE
                     IRDSGKVVGLDRVAVMAALNIANELLKAQGQDEELKNIVGLRIRAMREHLDSALGPTK
                     QLTL"
     gene            3271..3455
                     /gene="ssrS"
                     /locus_tag="VFL84_01395"
     ncRNA           3271..3455
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFL84_01395"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            3487..4044
                     /locus_tag="VFL84_01400"
     CDS             3487..4044
                     /locus_tag="VFL84_01400"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET7607305.1"
                     /translation="MRRELRAKRRALPAAARAQADRAIHAQLRALPEYRRARRLAVFM
                     AFDGEPSLRSLVETARRHGKQLYVPVLRGMTMRFAQLEPGAKLAANLFGILEPELGAS
                     IDARELDLVLTPLVGFDEHGTRLGVGRGYYDRTFKFLRHRSHWRRPKLLGVAYELQRV
                     QEPLAPNSWDVPLWGVVTEARVRRF"
     gene            4059..4526
                     /locus_tag="VFL84_01405"
     CDS             4059..4526
                     /locus_tag="VFL84_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HET7607306.1"
                     /translation="MAYWLMKSEPDVFGIDDLAKLKAKTTHWDGVRNFQARNFMRDGM
                     RRGDLAFFYHSSCDEPGVAGIVEIVKEGYPDSTAFDQNDPHFDPDSDRSKPRWYMVDL
                     KLVRKFRRTITLATLREHEQRALKGLALLKRGNRLSITPVSAAHWKFIVDLAE"
BASE COUNT          861 a         1654 c         1645 g          796 t
ORIGIN      
        1 taccgccggc gtcgtctccg ggcagcgtcc agatcacggc gccgcggccg cccgtgagcg
       61 gcagcagcgc gatcgggccg cggtcgctga agcgctcgaa cgcgcggccg tcgagccgcg
      121 cctcggtgct gcaattgaag acgacggcgc gctgcgcgta gtcgtcctcg gaagtcgcga
      181 cgccgagcgc cgcgcgcacg gcggaacgcg cgccgtcggc cgcgacgacg agcttcgcgc
      241 gcacgatttc cgccgcgccg ccgcgctcga ccgtgacctc ggcgtggccg ggctcttgct
      301 tcagcgcgac gaccttggcg ggcgcgagca ccgtcaggcg ccgcgtgtgc tcgaggcgtt
      361 cccagagcac gcggccgagc gtctgattct cgacggtgta gccgagcgcc ggcacgtgct
      421 cctcgcgggc gtcgatgcgc gcggcgccga agcggccgcg ctcggagatg tggatcgagc
      481 ggatcgactc cacgtacccc gcgagctcgc gccagagccc caagcccgac agaatttgct
      541 gcgagccgtt cgcgagcgcc gtgacgcgcg cgtcgaagct cggctgcgcg agcagctgag
      601 gcttcagcgc ctcgacgagc acgacgtcga gcggcagctc cccgagcgcg cacgcgagcg
      661 ccgacccgac ggcgccgccg cccgcgatca ggacgtcgca gtcgcgccgc gacgcggcgc
      721 tcacgctccc ctcccgttca tgtgccgctc gatctcggca accgtcgtcg gcacgccttc
      781 cgtcagcacc tcgggcccca agcgcgtgac ctgcacgtcg tcctcgatgc ggatgccgat
      841 gccgcgccaa cgcttgtcga cgctcttgtc gttggccgac acgtagacgc cgggctcgat
      901 cgtcatgacc atgcccggct cgagcagccg ccactggtcc gcgactttgt agtcgccgac
      961 gtcgtggacg tcgaggccga gccagtgtcc ggtcttgtgc atgaagaact gccgatacgc
     1021 gagcgacttc acgagctgcg gcacacggcc gtcgagcagg ccgagctcct tgagcccgcg
     1081 cgtcacgacg ccgactgcgg catcgtgcgg gtcgttccaa tgattgccgg ccttcgcttt
     1141 ggccgtcgcg gcgcggttcg catcgagcac gatttcgtag agctcgcgct gcggagcgct
     1201 gaaacggccg ttgacgggaa acgttcgcgt cacgtccgag gcgtacatgt cgagctcgca
     1261 gccggcgtcg atcaacacga gctcgccgtc gacgagcggc gcatcgttat tgcggtagtg
     1321 caggatgcac gcattggccc ccgccgcgac gatcggcgga tacgagcact cggcgccgtg
     1381 gcgccgaaac tcgtgcacga actcggcctc gagctcgtat tccttcatgc cggcgcggca
     1441 cgcgcgcatg gcgcggccgt gcgcgcccgc ggcgatgcgc gcggcctggc gcatcacgcc
     1501 ggtctcggag cggctcttga agagccgcat ctcgtgcagc agatgatcga gcgagacgag
     1561 ctcactcggc gcgtggccga tctggcgctt cgagttcaac gtctgaatga acgacagcag
     1621 cttctgatcg aactcgttgc cgccgatcga gtagtagacg cggtcgctgc gctcgagcag
     1681 cccgggcaag atgtcgtcca cgtccgagat cgggaaagcg tcgtcggcgc cgaactgcgc
     1741 cacggcgccc tctgggcccg cgcgcggccc gtcccactgc tcgcgctcgg gattgcgctc
     1801 gcggcagaac agcaggaact cgccttgcgg gcggcccggc acgagcacgg cgacggcctc
     1861 gggctcgcgg aagccggtca ggtagaagaa gtcgctgtcg ggccggtaga cgtagtgcgt
     1921 atcgcggctg cgcacgcgct cgggcgccgc cgcgagaacg gcgatgccat tagcgccgat
     1981 gagctccatc aagtgcctgc ggcggcgcgc gaattccttg gcgttcactg cttcgaaacc
     2041 ctcgccgtca gtgcaaatcg cgcgcggcgg ccctcacggg cgccagctcc tcaaatacga
     2101 gctgcacgct gacgcgcacg tattcgtgga tctgcgcgag cgcgaaatcc ggctggccct
     2161 cgccggcggc ctcctcgtcg ctcaaacccg cgcggctgat ctcggcgaag tcgtgcagga
     2221 cctcgcggaa cgcatcccct tcggccgcgg gcgccgtgct gccgacgccg ctcaagaagc
     2281 cttggcacca gaccgcgagc gcttcgactt gctccggcag cggcgcttcg tcgctcggca
     2341 gcagcggctc gaacgtgagc tcgtcgtctt cgagcatctt cgagctcgtg ccgatcagct
     2401 cttcgagatc ttccgcgagc gaggcagccg atgcgccgag ctcgacgtcg tcgagagaat
     2461 ccgtgagcca gcgccgcgcg gcgaccgagc cgcctgcgca caacgcgccg cacacgccgc
     2521 cgtgcagctc cgcgagcgcg acgacggcac ccgaagcggc gagcgaatcg cgcaggcgtt
     2581 cgtagtcgag cggaggagtt gcagagctcg gtgtcacgtg agcgctccgc gcaatgtgcg
     2641 cgcgctgaag gaaatgctag cacgcgcgtg ttgttgaccc tcgatcagcg cggttctata
     2701 ttgccgcgca tgacccccga ccaagcgaat catcgtgtcg acctcgaatt gaagaggctc
     2761 gagaaacggc tcgaagagct cgtcgtcgtt tgccgacagt tgcaggaaga aaacaggtcg
     2821 ctgcggcacc gtcaagattc tctgatgtcg gaacgcgcca cgctgctgca aaagaacgag
     2881 caggtgcgcg ggcgtgtcga agcgatgatt gggcggctca agggaatgga gcaggtttcg
     2941 tgagcggcac gggcacattt gctcacgtca cgatcaagat cctggagaaa gagtacaacg
     3001 tcgcgtgccc gatcgaggaa aagccccagc tgctcgcgtc ggccgagctc ctgaacgcga
     3061 agatgcgcga gatccgcgat agcggtaagg ttgtggggct cgaccgcgtg gccgtgatgg
     3121 ccgcgctcaa tatcgcaaac gagctgctca aggcacaagg gcaggatgaa gagctgaaga
     3181 acatcgtggg cctgcggatc cgcgccatgc gcgagcatct cgactcggct ctcggtccga
     3241 cgaagcagct cacactataa ttggccttgg tgctccctgc ggtgttcgac acggacgaag
     3301 gtctctcgga ccctaaatca tgcctttggg gttgaagcct gtcggtgatg gtgtgcaaat
     3361 ccggttgaac cggaaagcct taagtcctga cggtgtaccc cacctgttgc tccggttcga
     3421 gagcaacggt tcgtgacggc acaggcgggg agcatcactc cctcggcgga cccggtccgc
     3481 cgcgagctgc gccgcgagct gcgcgcgaaa cgccgcgccc tgcccgccgc cgctcgcgcg
     3541 caagccgatc gagcgatcca tgcgcagctg cgagctctcc ccgaataccg gcgcgcgcgc
     3601 cgcctcgcgg tgttcatggc gttcgacggc gagccgtcgc ttcggtccct cgtcgagacc
     3661 gcaaggcggc acggcaagca gctctatgtt cccgtcctgc gcggcatgac gatgcggttt
     3721 gcgcagctcg agcccggcgc gaagctcgcc gccaacttgt tcgggatcct ggagcccgag
     3781 ctcggcgcga gcatcgacgc gcgcgagctc gacctcgtcc tgaccccgct cgtggggttc
     3841 gacgagcatg gaacgcggct cggcgtcggc cgcggctact acgaccgcac gttcaaattc
     3901 ctgcgccacc gctcgcactg gcgcaggccc aagctcctgg gtgtggcgta cgagctacaa
     3961 cgcgtgcagg agccgttggc gccgaacagc tgggacgtcc cgctgtgggg agtcgtgacc
     4021 gaggcccgag tgcggcgctt ctagcggagg acttcggcgt ggcctattgg ctcatgaaat
     4081 cggaacccga cgtgttcggc atcgacgacc tcgccaagct caaggccaag acgacgcact
     4141 gggacggcgt ccgcaatttt caggcgcgca atttcatgcg agacggcatg cgccgcggcg
     4201 atctcgcgtt cttctatcac tcgagctgcg acgagcccgg cgtcgccggc atcgtcgaga
     4261 tcgtcaaaga agggtatccg gactccacgg cgttcgatca gaacgatccg cacttcgatc
     4321 ccgacagcga tagatcgaag ccgcgctggt acatggtcga cctgaaactc gtgcgcaagt
     4381 ttcgccgcac gatcacgctc gccacgttgc gagagcacga gcagcgcgca ctgaaagggc
     4441 tcgcgctgct gaaacgcggc aatcgtttgt ctatcacgcc ggtcagcgcc gcgcattgga
     4501 aattcatcgt cgatctcgcg gagtgaagcg cgaacgcgct gcgtgagctc gttcgtgatt
     4561 cgtttcgcga gagtgtgcaa ccaatcacac tcggtgttcg tttgcacacg atcacggtgc
     4621 gcatgtcaat gacgcgctgg aatttcgaat cgttttatat atactcaacc accgtctatc
     4681 ccgacaacgg agactaccca tggcttctgt gaagaaaacg tcaacgaaaa agaagacgcg
     4741 aagaggcaaa aagcgccgcg tcgcgcgacg cactgcgcga aaagtccggc gcaagggccg
     4801 ccgcggcggc aggaaggtac gacgcacggg gcgccgcgtc ggaagaaaag tccggcgcaa
     4861 ggctcgcagc aagcgctaac ggattagcaa cgtcagtcga gccacggtgt ggctcagaca
     4921 agtgtagtgc ccgcaaagtc gcgggcactc gctttt
//
LOCUS       DASSNN010000077         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1774165, whole genome shotgun sequence.
ACCESSION   DASSNN010000077 DASSNN010000000
VERSION     DASSNN010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774165"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..690
                     /locus_tag="VFL84_01410"
     CDS             136..690
                     /locus_tag="VFL84_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HET7607307.1"
                     /translation="MRSLRVARAARIVRAGGVIAYPTEAVFGLGCSPRYTEAVMRVLA
                     IKRRSWRKGLILIGADLGQLERYVVLPPEPRRSEVLATWPGPNTWVLDARPDAPRWIT
                     GGRASVAVRVTAHALAAELCRAAGEALVSTSANVSRRPPHRRLLTLRRDLGRSVDYVL
                     AGALGGLEAPTAIRDGRTGRLLRP"
     gene            908..>2091
                     /locus_tag="VFL84_01415"
     CDS             908..>2091
                     /locus_tag="VFL84_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7607308.1"
                     /translation="MAWLAAVMLMPAAAQPMHVQVTEPLRLVGPLSPVKGGQAVYIVK
                     LRQPGAASFKNLRTALMAEKPSEQARASRAAAAAAYARQLEQSHDRLLAGIGASNEKL
                     YSYRYSVNGFAARLTAAQVSRLAQSAEVERIWQDTDQHLNTNNSSRFLGLQNPVGGLR
                     ADLGLRGENIVVGVIDSGIAPNHPSLQDTEDRTPRACRSEWSRSSILGLWLCMAFHHD
                     PPTELVYDPPIGFTGACQAGPQFEATSCNNKVVGARFYLEGFLAQHELDPGEVRSPKD
                     ADGHGTHIATIVAGNSVTATLLGARVGRVSGIAPRARIAVYKACWLEPGATRGTCTTS
                     DLVRAIDDAVADGVDLINYSVGGLETDLDAPDDLALLDALDAGVLTVVAAGNDGPELD
                     TIG"
BASE COUNT          334 a          756 c          696 g          305 t
ORIGIN      
        1 gaagcaaaag aagaccaaga aaaagaagag gaaagccgcg gcgtcgccga aggccggcga
       61 cgcgggcgat acggcggccg acgcgagcga gacgacgccc ggcggcgaag gcgcgggcga
      121 cccgccggcg gagtgatgcg ctctttgcgc gtagctcgcg ccgcgcgcat cgtgcgcgcc
      181 ggcggtgtga tcgcgtatcc cacggaagcc gtcttcggct tgggctgcag cccgcgctac
      241 accgaagcgg tcatgcgcgt gctcgcgatc aagcgccgct cgtggcgcaa gggtctgatt
      301 ctgatcggcg ccgacttggg gcagctcgag cggtacgtcg tgctgccgcc cgagccgcgc
      361 cgcagcgagg tgctcgcgac ctggcccggc ccgaatacct gggtgctcga cgctcggccc
      421 gacgcgccgc gctggatcac gggcggccgc gcgagcgtcg ccgtgcgcgt gacggctcac
      481 gcgctcgcgg ccgagctctg ccgcgccgcc ggcgaagccc tcgtctcgac gagcgccaac
      541 gtgagccgcc gcccgccgca ccggcgcttg cttacgctgc gccgcgacct cggccgcagc
      601 gtcgactacg tgctcgcggg cgctctcggc ggcctcgaag ccccgacggc cattcgcgac
      661 ggccgcaccg gccgcctgct gcgcccctag cgctaaaagg ggacagatct attttcccag
      721 caccgctaaa aggggacaga tctattttcc cagcacagcg cggggacgga tccatttcgg
      781 cttctggaaa atagatctgt ccccttttag cgtgtcccct tttagcgctc gctagctgtg
      841 ctaacgtttc agcaccgcgg cgcaacggcc cgaattagca atagaaatgc cgatcctccg
      901 cagagccttg gcatggctcg ccgccgtcat gttgatgccg gcggcggcgc agcccatgca
      961 cgtgcaggtc acggagccgc tccggctcgt cggcccgctg tcgcccgtca agggagggca
     1021 agccgtctac atcgtgaagc tgcgccagcc cggggccgcg agcttcaaga acctgcgcac
     1081 ggcgttgatg gccgagaagc cgagcgagca agcgcgcgcg tcgcgcgccg ccgcggccgc
     1141 cgcctacgcg cgccagctcg agcagtcgca cgatcggttg ctcgccggta tcggcgcatc
     1201 gaacgaaaag ctctatagct atcgctactc ggtcaacggc ttcgcggcgc ggctgacggc
     1261 cgcgcaagtg tcgcggctcg cgcagagcgc cgaggtcgag cgcatctggc aggacaccga
     1321 tcagcacctg aacacgaaca atagctcgcg gttcctcggt ttacagaacc ccgtcggcgg
     1381 cttgcgcgcc gacctcgggc tgcgcggcga gaacatcgtc gtcggcgtca tcgacagcgg
     1441 catcgcgccg aatcatccgt cgctgcagga caccgaggac cgtacgccgc gcgcgtgccg
     1501 cagcgaatgg tcgagaagct cgatcctcgg gttgtggctc tgcatggcct tccaccacga
     1561 cccgcccacg gagctcgtct acgatccgcc gatcggtttc acgggcgcgt gccaagccgg
     1621 acctcagttc gaggcgacgt cttgcaacaa caaggtcgtc ggcgcccgct tctatctcga
     1681 aggcttcctc gcgcagcacg agctcgatcc cggcgaagtg cgctcgccga aggacgccga
     1741 cggccacggc acgcacatcg cgacgatcgt cgccggcaac tccgtcaccg cgacgctgct
     1801 cggcgcgcgc gtcgggcgcg tcagcggcat cgcgccgcgc gcgcgcatcg ccgtctacaa
     1861 agcgtgctgg ctcgagcccg gcgcgacgcg cggcacttgc acgacgtccg atctcgtccg
     1921 cgcgatcgac gatgccgtcg ccgacggcgt cgatctcatc aattactccg tcggcggcct
     1981 cgagaccgac ctcgatgcgc ccgacgacct cgcgctgctc gatgcgctcg acgccggcgt
     2041 gctgaccgtc gtcgcggccg gcaacgacgg cccggagctc gacacgatcg g
//
LOCUS       DASSNN010000078         4664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1531342, whole genome shotgun sequence.
ACCESSION   DASSNN010000078 DASSNN010000000
VERSION     DASSNN010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531342"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VFL84_01420"
     CDS             complement(<1..645)
                     /locus_tag="VFL84_01420"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET7607309.1"
                     /translation="MSRRTGTTIAAAAFAAVAACGWLGVARGQSADARLSAPPEAEFH
                     FARLVYRNSPYSRRGYYGRGGGAWTTDYPDAEYHVMQGIGRFTGIDIAEVDPDGNGGR
                     WIQLVDERVFDYPWLYAVEVGQWLLDESEAKILREYLVRGGFLMVDDFWGEREWEIFV
                     DSMQRVFPDKPIEELGEEHALLHVLYDLNQRTQIPGRGGPRPEKPHWRGIFDEDG"
     gene            776..1495
                     /locus_tag="VFL84_01425"
     CDS             776..1495
                     /locus_tag="VFL84_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HET7607310.1"
                     /translation="MRTGRVAPWVAIVVALASTGAARAQDPESELSGYATLSSGYWKH
                     GLSQIDGASLQLGIEYQHYSGFFAYARAMNVDYPQNLPGQTRNVETSAYVGYHDRGDR
                     WSWTVSLGRYVYPGAADYNYGEWSGSVGFRDRVFYTASYNDEYYARGTSALNQEISVA
                     FPLPANFEIGGAVGYFDISRGAQITHWNLGASKLLGRMAIDLRYYDGNYYYRNYLGDP
                     HASNYVLSVTSALKRRHGAAR"
     gene            complement(1492..2769)
                     /locus_tag="VFL84_01430"
     CDS             complement(1492..2769)
                     /locus_tag="VFL84_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7607311.1"
                     /translation="MKLVLLVAAALLAGCASSTGRRTDETAAAAEQTASRQILVTVRQ
                     PATLAAGLTGAPNQRYLQRRYGPAPSVDRVLTQLAHEHDLRRVEGWPIQSLDVYCEVL
                     SIPEGRNVAEVLDALNRDSRVDLAQRMNLFTTQTTKYDDPYLDLQSAATEMEVEAAHE
                     LATGRGVSIAIIDSAIDSDHPDLRGRVRLARNLVTEHPTARDGEIHGTAIAGVIASAV
                     NNREGIIGVAPDVSIAALRACWAISEDGPTAQCSSFSLARALELALGLQPNLINLSLA
                     GPDDPLLSRLLDRVIEQGIVVVAAYPATSESSYAFPSSHPHVLTAHSSKVRVNSDSPF
                     TLAAPASEVLTTTPGAGYAFLSGNSLAAAHTTGVIALLMERQPDIDAERIAAILTATT
                     TYSAGTASINACRAVARLAGSEPCLSPTANVRF"
     gene            complement(2766..3404)
                     /locus_tag="VFL84_01435"
     CDS             complement(2766..3404)
                     /locus_tag="VFL84_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607312.1"
                     /translation="MTEPAALSHTRAFELLPWLINGTLAGGEREAVEQHVRACIVCRR
                     ELKEQNALHAAVRARRTVDVSAEAGFDRLDRELDAVPGAASSRWRVRYGSAAAFALAT
                     AAGVAVLAVLLWLTPLPGLDRGGYETLATPPAEAALLDIVFADDTTAAQMQELLDRID
                     GEIVAGPTEVGRYSVRVVGDQAKRRKLDELLRVLAADPRVRFAGPSLADTEP"
     gene            complement(3401..4003)
                     /locus_tag="VFL84_01440"
     CDS             complement(3401..4003)
                     /locus_tag="VFL84_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7607313.1"
                     /translation="MDQGLNVRPARARFSDDTADLALLQRIANGDRTALRQLYTTYYH
                     PLLRFIYRITGRLDLAQEGVNDVMLVVWRNGRSFGGRSSVSTWIMGIGYRKALKLLAS
                     SRRWTDRFAGAPFDEIIERSDSLAEQSDDRDLRDLLDAALRHLSAEHRAVVELTYFYG
                     CSYEEIAAIAACPVNTVKTRMFHARAKLKKLLPALGKDPL"
     gene            4200..>4664
                     /locus_tag="VFL84_01445"
     CDS             4200..>4664
                     /locus_tag="VFL84_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7607314.1"
                     /translation="MTETDPASDDGVDVRLLAEIAAGVEQAFVELYRRRHNDVYRFAF
                     AMAKSRSFAQDVTQEVFLNVLENARRFDGAKGSVRAWLFGCARYVTLDRLRVERRWTD
                     DLPADLTALDSDERLLADQRIERLHAAIARLPVEYREALVLVELQELTYAETA"
BASE COUNT          768 a         1500 c         1618 g          778 t
ORIGIN      
        1 gccgtcctcg tcgaagatgc cgcgccagtg cggtttctcg ggccgcgggc cgccgcggcc
       61 gggaatctgc gtgcgttgat tgaggtcgta gagcacgtgc agcaaggcgt gctcctcacc
      121 gagctcctcg atgggcttgt cggggaacac gcgctgcatc gagtcgacga agatctccca
      181 ctcgcgttcg ccccagaagt cgtcgaccat gaggaaaccg ccgcggacga ggtactcgcg
      241 caagatcttg gcttcgctct cgtcgagcag ccactggccg acctcgacgg cgtatagcca
      301 cggatagtcg aacacgcgct cgtcgacgag ctggatccac cggccgccgt tgccgtcagg
      361 gtcgacttcc gcgatgtcga tgcccgtgaa ccggccgatg ccttgcatga cgtggtactc
      421 ggcatcggga tagtccgtgg tccacgcccc gccgccgcgg ccgtagtagc cgcgccggct
      481 atacggcgaa ttacggtaga cgaggcgggc gaagtgaaac tcggcctcgg gcggcgccga
      541 caggcgcgca tcggcggact ggccgcgcgc gacgccgagc cagccgcagg ccgcgacggc
      601 ggcgaacgcg gccgcggcga tcgtcgttcc ggtacggcgg ctcatgccag gtagcgtaac
      661 ccagccgcat gccgaccccg aatagcgctg cgccccgtgg gtccttgggc ccggtagcgc
      721 ggcacggccg cgcaggccta gaatcgaatc gatcgtctgc gggaggagct cattcgtgcg
      781 tactgggcgc gtggcgccgt gggtcgcgat cgtcgtcgcg ctggcttcga ccggcgccgc
      841 gcgcgctcaa gatccggagt cggagctcag cggctacgcg accttgagca gcggttactg
      901 gaagcacgga ctgtcgcaaa tcgacggcgc gtcgttgcag ctcggcatcg agtatcagca
      961 ctacagcggg ttcttcgcct acgcccgtgc gatgaacgtc gactatccgc agaaccttcc
     1021 gggccagact cgcaacgtgg agacgagcgc ctatgtcggc tatcacgatc gcggtgaccg
     1081 ctggtcgtgg accgtcagtc tcggccgcta tgtctatccc ggtgcggccg actacaacta
     1141 cggcgagtgg agcggcagcg tcggtttccg cgatcgcgtc ttctacaccg cttcgtacaa
     1201 cgacgagtat tacgcgcgcg gaacttcggc gctgaaccag gaaatctcgg ttgcgttccc
     1261 gttacccgca aacttcgaga tcggcggcgc ggtcgggtat ttcgacatct ccagaggcgc
     1321 gcagattacg cattggaacc tcggcgcctc gaagctcttg ggaagaatgg cgatcgattt
     1381 gcgctattac gacggcaact actattaccg caattacttg ggcgacccgc acgccagcaa
     1441 ctacgtgctc agcgtcacgt cggcgctgaa gcgaaggcac ggtgccgcgc gctagaagcg
     1501 cacgttcgcg gtcggcgaga ggcagggctc cgagccggcg agccgcgcga cggcgcggca
     1561 agcgttgatc gaagccgttc ccgcggaata ggtcgtcgtc gcggtcagga tcgccgcgat
     1621 tcgctcggca tcgatgtccg gctggcgttc catcagcagc gcgatgacgc ccgtcgtgtg
     1681 cgcggccgcg agcgagttgc cgctcaagaa ggcgtagcct gctcccggcg tcgtcgtcag
     1741 gacctcgctc gccggcgcgg ccaacgtgaa cggcgaatcg gaattcacgc gaactttcga
     1801 ggagtgcgcc gtcaacacat gcgggtgcga cgacggaaat gcgtacgagc tctcggacgt
     1861 cgcggggtac gcggcgacga cgacgatgcc ttgctcgatc acgcgatcga gcagccgcga
     1921 cagcagcgga tcgtcgggcc ccgccaagct caaattgatg agattcggct gcagaccgag
     1981 cgcgagctcg agcgcgcgcg cgagcgagaa gctcgagcac tgcgctgtgg gcccgtcttc
     2041 gctgatggcc cagcaggcgc gcagagcagc gatgctgacg tcgggcgcga ctccgatgat
     2101 cccttcgcga ttgttcacgg cggacgcgat gacgccggcg atcgcggtgc catggatctc
     2161 gccgtcgcgc gcggtcggat gctccgtgac gaggttgcgc gcgaggcgca cgcgcccgcg
     2221 caggtcgggg tggtcactgt cgatggcgga gtcgatgatc gcgatcgaaa cgccgcgccc
     2281 cgtcgcgagc tcgtgcgcgg cctcgacctc catttccgtc gcggccgatt gcaagtcgag
     2341 gtacggatcg tcgtacttcg tggtctgcgt cgtgaacagg ttcatgcgct gcgcgagatc
     2401 gacgcgcgaa tcgcggttca aagcgtcgag gacttcggcg acgttgcggc cttccggaat
     2461 ggaaagcacc tcgcaataga cgtcgagcga ttgaatcggc cagccttcga cgcggcgcaa
     2521 gtcgtgctcg tgcgcgagct gcgtcaaaac tcggtcgacg ctcggcgccg gaccgtagcg
     2581 gcgctgcaga tagcgctgat tcggagcgcc cgtgagcccc gcggcgagcg tcgcgggttg
     2641 gcgcacggtg acgagtatct ggcgcgacgc cgtctgctcg gcggccgcgg cggtttcgtc
     2701 ggttcgcctt ccggtcgagc tcgcgcaacc cgcaagcagc gccgcggcca cgaggagcac
     2761 gagtttcatg gctccgtgtc cgcgagcgac gggccggcga agcgaacgcg cgggtcggcg
     2821 gcgaggacgc gcagaagctc gtcgagcttc ctgcgtttgg cctggtcgcc gacgacgcgc
     2881 acgctgtaac ggccgacctc ggtcggtccc gcgacgattt cgccgtcgat gcgatcgagc
     2941 agctcctgca tctgcgcggc cgtcgtgtcg tccgcgaaga cgatgtcgag cagcgcggct
     3001 tcggccggcg gcgtcgcgag cgtttcgtat ccgccgcggt cgaggccggg cagcggcgtc
     3061 aaccacaaga gcacggcaag caccgcgacc ccggcggcgg ttgcgagcgc gaacgcggcg
     3121 gcgctgccgt agcggactcg ccagcgtgac gacgcggcgc cgggaacggc gtcgagctcg
     3181 cggtcgaggc gatcgaagcc ggcttccgcg gaaacgtcga cggttcgccg cgcgcgaacg
     3241 gcggcgtgca gcgcgttctg ctctttgagc tctcgccggc acacgatgca cgcgcgcacg
     3301 tgctgctcga cggcctcgcg ctcgcctccc gcgagcgtgc cgttgatcag ccagggcagg
     3361 agctcgaatg cgcgcgtgtg tgacaacgcc gcgggctccg tcacagcggg tccttgccca
     3421 gcgccggcag aagcttcttc agcttcgcgc gggcgtggaa catccgtgtc ttgaccgtat
     3481 tgacgggaca agcggcgatg gcggcgatct cctcgtatga gcagccgtaa aagtacgtga
     3541 gctccacgac ggcgcgatgc tctgccgaca ggtgacgtaa cgcggcatcg agcaggtcgc
     3601 ggaggtctcg gtcgtcgctt tgttccgcca gcgagtcgga acgttcaatg atctcgtcga
     3661 acggggcgcc cgcgaaccga tcggtccagc ggcgcgacga ggccaatagt ttcaaggcct
     3721 tgcggtagcc gatacccatg atccaggtcg atacgctcga gcgcccgccg aacgaacgcc
     3781 cgttgcgcca gacgacgagc atcacgtcgt tgacgccttc ctgggcgaga tcgagccggc
     3841 ccgtgatgcg gtagatgaac cgcagcagcg gatggtagta cgtggtgtag agctgccgca
     3901 gggcggtgcg gtcgccgttc gcgatgcgct gcagcaacgc gagatcggcg gtgtcgtcgg
     3961 agaaccgcgc ccgggcagga cgaacgttca gcccctgatc caatcgttct ccgttcacgt
     4021 ttccgccgac gaggctttag tctacgcacg ccgaagacaa gttgcacaac ttggccgcgc
     4081 ggggcgctct cggcggcagg ccgagcgctc accgggtttt gtgaaatctc gagtcggagc
     4141 gcctacctgg aacttttggc ggggctcgcg cgaatagctt ctgaggaaga cagctgcgca
     4201 tgaccgaaac cgatccagct tcggacgacg gagtcgacgt ccgtctgctc gccgagattg
     4261 cggcgggcgt cgagcaggct ttcgtcgagc tttacagacg ccggcacaac gacgtctatc
     4321 ggttcgcgtt cgcgatggcc aagtcgcgca gcttcgcgca agacgtgacg caagaggtgt
     4381 tcttgaacgt actcgagaat gcgcggcgct tcgacggcgc caaaggctcc gtgcgtgcgt
     4441 ggctgttcgg ctgcgcgcgt tacgtgacgc tcgatcggtt gcgcgtcgag cgccgttgga
     4501 ctgacgactt accggccgat ctcacggcgc tcgacagcga cgagcgcttg ctcgcggacc
     4561 agcgcatcga acggctgcat gcggcgatcg cgagattgcc cgtcgagtat cgcgaggcgc
     4621 tcgtgctcgt cgagcttcag gaattgacct atgccgagac ggcg
//
LOCUS       DASSNN010000079         2685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1648021, whole genome shotgun sequence.
ACCESSION   DASSNN010000079 DASSNN010000000
VERSION     DASSNN010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648021"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VFL84_01450"
     CDS             <1..1087
                     /locus_tag="VFL84_01450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET7607315.1"
                     /translation="AEDLAAGPVPAPRLASKSHYLIDFTTKKVLSASGADVQLPPASL
                     TKLMTAYVVFGALQSGRIRLDDRAHVSTKAWRTGGTRMFIEVNSDVALEDLLRGLLIQ
                     SGNDAAVALAEHVAGSVDAFVAEMNAAARKLGMQNTAFRNPHGLPTRGHYTTARDLAV
                     LAKAIIDEFPDFYSLYAEREFSYNGIAQHNRNALLWRDPSVDGMKTGYTASAGYCLVT
                     SAKRGGMRLIAVVLGAPTPRVRNDGAQKLLEYGFANFETHKLYSAGQELDNARVWGGE
                     AEFVHLGLTEDIYVTIPRGGYPKLAASMDVLAQLAAPLSRGTKVGEVKISFDGAPLAK
                     SPLVVLTNVLDGGVWARVRDELELIWE"
     gene            1084..1347
                     /locus_tag="VFL84_01455"
     CDS             1084..1347
                     /locus_tag="VFL84_01455"
                     /inference="COORDINATES: protein motif:HMM:NF016262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HET7607316.1"
                     /translation="MSGSDESPLKFPAELPIKVFGRNDAEFRAAVVAIIEKHYGTAYT
                     ITEVASKQAAYVSLTITVRAESRAQVDAVYQDFVASERVLMAL"
     gene            1418..1975
                     /gene="lipB"
                     /locus_tag="VFL84_01460"
     CDS             1418..1975
                     /gene="lipB"
                     /locus_tag="VFL84_01460"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008900371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET7607317.1"
                     /translation="MQAFTAERAGDTRDEIWFVEHPPVYTLGMRASREHLLAPGDIPV
                     VQIDRGGQVTYHGPGQLMVYVLLNLERRALTPRKLVQTLESAVIDTVAGYGITAVARR
                     DAPGVYVDGAKLAAVGLRVKRHCSYHGMAVNVAMDLTPFAGINPCGYANLPVTDLRAL
                     AGIESVARFRESFEPRLLARLAPSC"
     gene            complement(1953..>2685)
                     /locus_tag="VFL84_01465"
     CDS             complement(1953..>2685)
                     /locus_tag="VFL84_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607318.1"
                     /translation="SQLVISPNRGSRDYGVYVSWALATLLIAIFLENESLPPGTATLH
                     YAFVPQIVALMSLHVWISYRLEPWLVTLGASAAAATIVVGALLGVTTDLVGPAYWVAL
                     SLLGVLLAFLWWRAISTQRAFVNASSIYLQSKETLGTTSAPQKPWLGLPQWVGLIAAS
                     LGLGAANALLRGRAIEDLPAVEVAAESGLLMAMTALICVVPAGAYWVMRKAWMPELTR
                     FVWLAWIVVGFALTYGNYLSSLAPA"
BASE COUNT          460 a          928 c          909 g          388 t
ORIGIN      
        1 ggccgaggat ctcgcggccg ggccggtccc ggcgccgcgt ctcgcttcga agagccacta
       61 cctgatcgac ttcacgacga agaaggtgct gtcggcgagc ggggccgacg tgcagcttcc
      121 gcccgcgagc ctcaccaagc tgatgaccgc gtacgtcgtg ttcggcgcgc tgcagagcgg
      181 ccgcatccgg ctagacgacc gcgcgcacgt cagcacgaag gcctggcgca cgggcggcac
      241 gcgcatgttc atcgaggtca actccgacgt ggctttagag gacctgctgc gcggcctgtt
      301 gatccagtcc ggcaacgacg ccgccgtcgc gctggccgag cacgtcgcgg gctcggtcga
      361 cgcgttcgtc gccgagatga acgccgcggc gcgcaagctc ggcatgcaga acacggcgtt
      421 ccgcaatccg cacgggctcc cgacgcgcgg gcattacacg acggcgcgcg atcttgccgt
      481 gctcgcgaag gccatcatcg acgagttccc cgacttctac agcctctacg ccgagcgcga
      541 gttcagctac aacggcatcg cgcagcacaa ccgcaacgcg ctgttgtggc gggatccgag
      601 cgtcgacggc atgaaaacgg gctacacggc gtcagccggc tactgcctcg tcacgtcagc
      661 gaagcgcggc ggaatgcgcc tgatcgccgt cgtgctcggc gcgccgacac cgcgcgtgcg
      721 caacgacggc gcacagaagc tgctcgagta cggcttcgcg aacttcgaga cgcacaagct
      781 ctattccgcc gggcaagagc tcgacaacgc gcgcgtctgg ggcggcgagg ccgagttcgt
      841 gcatctaggc ctcaccgagg acatctacgt cacgattccg cgcggcggct atccgaagct
      901 cgccgcgagc atggacgtgc tcgcgcagct cgcggccccg ctctctcgcg gcacgaaggt
      961 cggcgaggtc aagatttcgt tcgacggcgc cccgctcgcc aagagcccgc tcgtcgtgct
     1021 cacgaacgtc ttggacggcg gcgtctgggc gcgcgtgcgc gacgagctcg agctgatctg
     1081 ggaatgagcg gctccgacga gtcgccgctg aagtttccgg ccgagctccc gatcaaggtg
     1141 ttcggccgca acgacgccga gtttcgcgcg gccgtcgtcg cgatcatcga gaagcactac
     1201 ggcacggcct acacgatcac cgaagtcgcg agcaagcaag cggcctacgt gagcctcacg
     1261 attaccgttc gcgccgagag ccgcgcgcag gtcgacgccg tctaccaaga cttcgtcgcg
     1321 agcgagcggg ttctgatggc gctatgagaa gcgcagcgtg gacgcgccgc tggttcgcga
     1381 cttagggttc cgtccgctgc cggacgtctg ggcgaagatg caggcgttca cggcggagcg
     1441 cgccggcgat acgcgcgacg agatctggtt cgtcgagcac ccgcccgtct atacgctcgg
     1501 catgcgcgcg agccgcgagc acctgctcgc gcccggcgac atcccggtcg tgcagatcga
     1561 tcggggcggg caggtgacgt accacggccc gggccagctc atggtctacg tcttgctgaa
     1621 cctcgagcgg cgcgcgctca cgccgcgcaa gctcgttcaa acgctcgaga gcgcggtcat
     1681 cgacacggtc gcgggttacg gaatcacggc agtcgcgcgc cgcgacgcgc ccggcgtcta
     1741 cgtcgacggc gccaagctcg cggccgtcgg cctgcgcgtc aagcgccact gctcgtatca
     1801 cggcatggcc gtcaacgtcg cgatggatct tacgccgttc gcgggcatca atccgtgcgg
     1861 ctacgcgaac ctgcccgtga cggatctacg cgcgctcgcc gggatcgaga gcgtcgcgcg
     1921 ctttcgcgag agcttcgagc cgcgcttgct cgctaggctg gcgccaagct gctgagatag
     1981 ttgccgtagg tcagcgcgaa gccgacgacg atccacgcga gccagacgaa gcgcgtgagc
     2041 tcgggcatcc acgccttgcg catgacccag taggcgccgg cgggcacgac gcagatgagc
     2101 gcggtcatcg ccatcagcaa gccggactcg gcggcgacct cgacggccgg caggtcctcg
     2161 atcgcgcggc ctcgcagcag cgcgttcgcc gcgccgagcc ccaagctcgc cgcgatcaag
     2221 ccgacccatt gcggcaggcc gagccacggc ttttgcggcg ccgacgtcgt cccgagcgtc
     2281 tctttggact ggagatagat cgagctcgca ttgacgaacg cgcgctgcgt cgagatggcc
     2341 ctccaccaca agaaggcgag cagcacgccg agcagcgaca gtgcgaccca gtacgccggc
     2401 ccgacgagat ccgtcgtgac gccgagcagc gcgccgacga cgatcgtcgc ggcggccgcg
     2461 gacgcgccga gcgtcacgag ccacggctcg aggcgatacg agatccagac gtgcaggctc
     2521 atgagcgcca cgatctgcgg cacgaacgcg tagtgcaacg tggcggtgcc gggcggcagc
     2581 gattcgttct cgaggaaaat cgcgatcagc aacgtcgcga gcgcccacga cacgtagacg
     2641 ccgtaatcgc gcgagccgcg attcggcgag atcacgagct ggctg
//
LOCUS       DASSNN010000080         4445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_891428, whole genome shotgun sequence.
ACCESSION   DASSNN010000080 DASSNN010000000
VERSION     DASSNN010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891428"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1400
                     /locus_tag="VFL84_01470"
     CDS             174..1400
                     /locus_tag="VFL84_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7607319.1"
                     /translation="MRFLIIDDDAGYRQVLRYHLEVEWPDAVIDEHQPSSAGFSEQDF
                     DLVGVDLVLLGHPLVGHEGFEFLKSLRRRADCPPVLLFAQVSDEFLAVDALKSGAANF
                     FPKDRVKHQRLIEVVRGELRVHRLDSTGELLANHKVLNGPRRRRVIAKLYAGDLSSVY
                     LAESEDGGERIALKVLRHVPDVGGGRLFDRFLQEYEVIARVRHANVVRIFDLGVADDH
                     AYIAMEYLGAGSLGERLKQALAPERAVDYLRQIAGALAAIHEAGILHRDLKPANIMFR
                     DEGSLALIDFGLAKQMRLHAAITGTGQIFGTPYYMSPEQGHAEPTDERSDLYSLGCIL
                     YEMLTGERPFTSNSPMGVIYKHAHAPRPRLSRALTHFQPVLDRLLAVDRFERYQSARE
                     LLAALDEHAPAHRAAR"
     gene            complement(1397..2116)
                     /locus_tag="VFL84_01475"
     CDS             complement(1397..2116)
                     /locus_tag="VFL84_01475"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HET7607320.1"
                     /translation="MTTLFISDLHLTAGDAETMRRFVEFMDGPARAARELYILGDLFE
                     AWIGDDDDDPRLEPIVVALKRLTDSGVACSLMHGNRDFLLGPRFCEATGTRLLGDYER
                     ITLDGQPVLLTHGDLLCTDDTRYMTLRAELRSKSWQRDFLARPLAERRQIASDLRQLS
                     ATEVAAKDDYIMDVNQGTVERTMREHDVTLLVHGHTHRPAVHRFSLEGRPAARIVLGA
                     WYHDPSIMRWTEAEFALEQLD"
     gene            complement(2113..2700)
                     /locus_tag="VFL84_01480"
     CDS             complement(2113..2700)
                     /locus_tag="VFL84_01480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7607321.1"
                     /translation="MRKLSLLLCLLFLPQALLAQDGASPADGQRVRVVTTAGNFVIEL
                     DHNRAPRTVDAFLKYVKEGFYSGVIFHRVVAGFIAQAGGYTADMQPKPVTENVVNESG
                     NGLSNLRGTVGFARSAEPHSGTSQFYINLADNVDLNPRPTRWGYAVFGKVVEGMEVVD
                     DIGHRPTAGGGPFQSSVPVEPIVIERIELIETAAK"
     gene            complement(2778..3704)
                     /locus_tag="VFL84_01485"
     CDS             complement(2778..3704)
                     /locus_tag="VFL84_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HET7607322.1"
                     /translation="MTATLLDGKAVAKNIRDELAARVTNVRERLGRPPALAIVLVGDD
                     PASEVYVRNKLKTAEDAGMGVSLERLPSSTSLAAVLAVVDRPNKDPKFDGILVQSPLP
                     KALGADAEQRVFDTIDPNKDVDGFSPVSAGRVIQNREGFAPCTPAGVIELLVRTKTPI
                     EGKHAVVIGRSDIVGKPMAALLLHKNATVTICHSRTRDLPAMARTADILVAAIGRPAF
                     VTPDFVKPGAVVVDVGINSVTSADEAKRIFARYPERLQVFEKKGSVLVGDVHPDVAEK
                     AGALTPVPGGVGPLTIAMLMANTVRAAELRLR"
     gene            3833..3909
                     /locus_tag="VFL84_01490"
     tRNA            3833..3909
                     /locus_tag="VFL84_01490"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3867..3869,aa:Pro,seq:tgg)
     gene            3930..4005
                     /locus_tag="VFL84_01495"
     tRNA            3930..4005
                     /locus_tag="VFL84_01495"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3963..3965,aa:Arg,seq:tct)
     gene            4039..4114
                     /locus_tag="VFL84_01500"
     tRNA            4039..4114
                     /locus_tag="VFL84_01500"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4072..4074,aa:His,seq:gtg)
     gene            4128..4203
                     /locus_tag="VFL84_01505"
     tRNA            4128..4203
                     /locus_tag="VFL84_01505"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4161..4163,aa:Lys,seq:ctt)
BASE COUNT          745 a         1428 c         1472 g          800 t
ORIGIN      
        1 caaaaagggg acagatctat tttctcggac gcaacgcgca aaaaggggac ggatctattt
       61 tcttgggaaa acgcgtttgt cccgcggtgc tcttctgaaa atagatccgt cccctttttg
      121 aagaaaatag atctgtcccc attttgagca gtcccctttt tgagcagcgt cacttgcgat
      181 tcttgatcat cgacgacgac gcgggctaca ggcaggttct ccgttatcac ctcgaggtcg
      241 aatggcccga tgccgtcatc gacgagcatc agccgagctc ggccgggttc agcgagcagg
      301 atttcgactt ggtcggcgtc gatctcgtgc tgctcggcca tcctctcgtc ggccacgaag
      361 gcttcgaatt cctgaagtcg ttacgccggc gcgccgattg cccgccggta ttgctgttcg
      421 cgcaggtcag cgacgagttc ctcgccgtcg acgcgctgaa gtccggcgcg gcgaacttct
      481 tcccgaaaga ccgcgtcaag caccagcgcc tcatcgaggt cgtgcgcggc gagctgcgcg
      541 tgcaccggct cgattcgacg ggcgagctgc tcgcgaatca caaggtgctg aacggccctc
      601 gcaggcgccg cgtcatcgcg aagctctatg ccggcgatct gtcgtcggtg tatctcgcgg
      661 agtccgagga cggcggcgag cgcatcgcgc tcaaggtgct gcggcatgtc cccgacgtcg
      721 gcggcgggcg gctgttcgac cgcttcctgc aggaatacga ggtcatcgcg cgcgtgcgcc
      781 acgcgaacgt cgtgcgcatc ttcgatctcg gcgtcgccga cgaccacgcg tacatcgcga
      841 tggagtactt gggcgccggc agcctcggcg agcggttgaa gcaagcgctc gcgcccgaga
      901 gggccgtcga ttacttgcgg cagatcgccg gagcgctcgc ggcgattcac gaagccggca
      961 tcctgcaccg cgacctcaag cccgcgaaca tcatgttccg cgacgagggc agcctcgcgc
     1021 tgatcgattt cggcttggcg aaacagatgc ggctgcacgc cgcgatcacg gggacggggc
     1081 agatcttcgg cactccttac tacatgagcc ccgagcaagg tcatgcggag ccgaccgacg
     1141 agcgcagcga cctctatagc ttgggatgca ttctctacga gatgctgacg ggcgagcgcc
     1201 cgttcacgtc gaattcgccg atgggcgtga tctacaaaca cgcgcacgcg ccgcggccgc
     1261 gcctctcccg cgcgctgacg cacttccagc ccgtgctcga ccggctgctc gccgtcgacc
     1321 gcttcgagcg ctatcaatcg gcgcgcgagc tgctggccgc gctcgacgag cacgcgccgg
     1381 cccaccgagc ggcacgctag tcgagctgct cgagcgcgaa ctcagcttcc gtccagcgca
     1441 tgatgctcgg gtcgtgatac caggcgccca gcacgatccg cgccgcgggg cggccttcca
     1501 gactgaagcg gtgcacggcg ggccgatgcg tgtggccgtg cacgagcagc gtcacgtcgt
     1561 gctcgcgcat cgtgcgctcc accgtgccct gattgacgtc catgatgtag tcgtccttgg
     1621 cggcgacctc ggtcgcggag agctggcgca agtcgctcgc gatctggcga cgctcggcga
     1681 gcgggcgcgc gaggaagtcg cgctgccagc tcttcgagcg aagctccgcg cgcagcgtca
     1741 tgtagcgcgt gtcgtcggtg cagagcaagt cgccgtgcgt cagcagaacg ggctgaccgt
     1801 cgagcgtgat gcgctcgtag tcgccgagca gacgcgtgcc ggttgcctcg caaaaccgcg
     1861 ggccgagaag aaagtcgcga ttgccgtgca tgagcgagca tgcgacgccg gaatcggtga
     1921 gcctctttag cgcgacgacg atcggctcga ggcgagggtc gtcgtcgtcg tcgccgatcc
     1981 atgcttcgaa caagtcgccg aggatgtaga gctcgcgcgc ggcgcgcgcg ggcccgtcca
     2041 tgaactcgac gaatcgccgc atcgtctccg cgtctcccgc agtcaagtgc aggtcggaga
     2101 tgaagagcgt ggtcacttcg cggctgtctc gatcagctcg atgcgctcga tgacgatcgg
     2161 ctcgacgggg acgctgcttt ggaacggacc gccgcctgcc gtcggccggt ggccgatgtc
     2221 gtcgacgacc tccatgccct cgacgacctt gccgaacacg gcgtaacccc agcgcgtcgg
     2281 ccgcggattc aggtccacgt tgtcggcgag gttgatgtag aactggctcg tgcccgaatg
     2341 gggctccgcg ctgcgcgcga acccgaccgt gccgcgcaag ttgctcaagc cgttgcccga
     2401 ttcgttgacg acgttttccg tgacgggctt cggctgcatg tcggccgtat agccgccggc
     2461 ttgcgcgatg aaccccgcga cgacgcggtg gaagatgacg ccggaataga agccttcctt
     2521 cacgtacttc aagaacgcgt cgaccgtgcg cggcgcgcga ttatggtcga gctcgatgac
     2581 gaagttgccc gcggtcgtga cgacgcgaac gcgctggccg tcggccgggc tcgccccgtc
     2641 ctgggcgagg agcgcctgcg gcaggaacag caggcagagc aacaggctca atttgcgcat
     2701 gggcttcgac ctcgagctca agcctcgtaa gaacggcctc ttcgactcat gataacggct
     2761 cggcgttcct cggcgagcta gcggagccgc agctcggccg ccctgacggt gttcgccatc
     2821 agcatcgcga tcgtcagcgg cccgacaccg cccggcaccg gcgtcaatgc gcccgccttc
     2881 tcggcgacgt cgggatgcac gtcgccgacg agcacggagc ccttcttctc gaagacctgc
     2941 agccgctcgg ggtagcgcgc gaagatgcgc ttcgcttcgt cggccgacgt gacggagttg
     3001 atgccgacgt cgacgacgac ggcgcccggt ttgacgaagt cgggcgtcac gaaggcgggc
     3061 cggccgatcg cggcgacgag gatgtcggcc gtgcgcgcca tcgccggcaa atcgcgcgtg
     3121 cgcgagtggc agatcgtgac ggtcgcgttc ttgtgcagca gcagggccgc catcggcttg
     3181 ccgacgatgt cggagcgccc gatcacgacg gcgtgcttgc cttcgatcgg cgtcttcgtc
     3241 cgcacgagca gctcgatgac gccggccggc gtgcacggcg cgaagccttc gcggttctgg
     3301 atcacgcgcc ccgccgagac ggggctgaag ccgtcgacgt ccttgttggg atcgatcgtg
     3361 tcgaagacgc gttgctcggc atcggcgccg agcgctttcg gcagcggcga ctgtacgaga
     3421 atgccgtcga atttcgggtc cttgttcggg cggtcgacga cggcgagcac ggctgcgagc
     3481 gaggtcgagc tcggcagccg ctcgaggctc acgcccatgc cggcgtcctc ggccgtcttc
     3541 agcttgttgc gcacgtagac ctcggacgcc gggtcgtcgc ctacgagcac gatcgcgagc
     3601 gcgggagggc ggccgagccg ctcgcgcacg ttcgttacgc gagccgcgag ctcgtcgcgg
     3661 atgtttttgg cgacggcctt gccgtcgagc agcgtcgcag tcacgtgcac cttgagtagc
     3721 ctaggaggga cgcggatttt cgcacgagct tcttcggccg ctcaaggcaa cggcgacgcg
     3781 ttcgtttgcc gcggcatcgg cggcggcata agatgccccg ctgaaagtga aacggggcat
     3841 ggcgcagcct ggtagcgcat ctgctttggg agcagagggt cgcaggttca aatcctgctg
     3901 ccccgaccaa aataacctcg atgaacgttg cgcccgtagc tcaagggata gagcaccggc
     3961 cttctaagcc gggggttgca ggttcgaatc ctgccgggcg cgccatgact catacgatag
     4021 aatgacgaaa acacgctggt ggacgtagct cagttggtag agccccggat tgtgattccg
     4081 gccgtcgtgg gttcgagtcc catcgtccac cccactttcc gacccgcggg ccgttagctc
     4141 aattggtaga gcagtggact cttaatccat tggttgtagg ttcgagtcct acacggccca
     4201 ccacttaatc aacgcgttac gccggttcag gggccttact gaaccggtaa ttcccaaaac
     4261 acgcggccaa ttcccaaaac ccgtttaaca gggcgagagg gtgacccact tcttgaatca
     4321 tggcccgagc tttctggtat aggtggtggt cgcgggagca cccaggggtt actcacgttg
     4381 ggaagagccg gcgccattct gatcgtcatt gcggccatcg cgctgccgtc ggtcggtagc
     4441 gcgca
//
LOCUS       DASSNN010000081         3256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1531510, whole genome shotgun sequence.
ACCESSION   DASSNN010000081 DASSNN010000000
VERSION     DASSNN010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531510"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFL84_01510"
     CDS             <1..231
                     /locus_tag="VFL84_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7607323.1"
                     /translation="FVQQGLALSAVGAVIGLVAAAGLGRLMSSLLFGVGPLDPVAYAA
                     ALGATIAAAGVASYVPARRAAKIDPMNTLRAE"
     gene            272..2944
                     /locus_tag="VFL84_01515"
     CDS             272..2944
                     /locus_tag="VFL84_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607324.1"
                     /translation="MHDWQSEVRARLAALRLKPEREADIVDEIAQHLAERYREATQAG
                     ASREDATRAALAEFQAGNALAQRIAALKQAHAPATVTPGASTGHLLADLVQDLRYAAR
                     AFAKKPGFAATAVLILALGLGATTAIFSLVYGVLLKPLPFPEPQALVSLRHYANFGET
                     SQGPATYFTYRENQRSFEDIGAWDQATVAITGGGETPERVEALIVSSSTLQLLRAQPR
                     LGRFFGTDDDVIGSAPRAILTAGYWQRRFGGAEDILGRVITIDGTPTEIIGVLPPSFE
                     FLSTRPGVLLPMPLNPNARWISFGFNAVARLKPGVTPEQASADVEHMISLLPSIFAPL
                     ELRPNVRPLADDVTGNVADVLWILLAAVGVVLLIACGNVANLFLVRAEARHQELATRA
                     ALGASRGRLARTLLAESVALALAGGVLGVALASLGLALLRAIGPAQLPRLEEVGIDPA
                     VLLFAAAVSIVSGVLFGMFAVARYGNPGIAMLKSGSRAASESRERNRARNAVVIGQVA
                     LALTLLIVSGLMARTLVAMLGVDPGFTLPKQVQTFVVAVPDTLIADDEQAARTHQSIA
                     ERLQQVPGVLSVGLSSSITMDGEDNGNYLTADGLPDKGLEGPQRRFKSVGPGYFETMG
                     NPIVAGRSVEWSDVLARRHPVVISETLARELWQEPSEAIGSRVRCCGNEEWSEIVGVS
                     GDERDDGLNRPATAIVYWAMLNDSYRWRAMAYAVRSERVGTPGFMRELEQAVWNVNPD
                     LPLASIQTLEQIQTTSMAKTSFTLVMLVIAASVALLLGVVGIYGVISYVAAQRTREIG
                     IRIALGAKLADVRKLFLSHGLWLTGSGIALGLVLAVVVTRVMSSYLFGVGPVDPLTYA
                     AVSALLAAISLLAIYLPARRAARIDPNVALRADT"
     gene            2973..>3256
                     /locus_tag="VFL84_01520"
     CDS             2973..>3256
                     /locus_tag="VFL84_01520"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HET7607325.1"
                     /translation="MHDWKSEVRARLAPLRLKPEREADIVDEIAQHLAERYREAISGG
                     ASHQEATRVALAEFQAGNALAQRIAALKQAHAPAPVTAGASTGRLFADLK"
BASE COUNT          497 a         1113 c         1125 g          521 t
ORIGIN      
        1 ttcgtgcagc aagggctcgc gttgagcgcc gtgggtgccg tcatcgggct cgttgcggcc
       61 gcggggctgg gccgcttgat gtcgtcgttg ctgttcggcg tcggcccgct cgatcccgtt
      121 gcctacgccg cagctctcgg cgccacgatc gcggcggcag gggtcgcgag ctacgtgccg
      181 gcgcgccgcg cggcgaagat cgatccgatg aacacgctca gggccgaata accgcaggcg
      241 ggcatctgct gcatgatcac cggagcccac catgcatgac tggcagagcg aggtccgcgc
      301 gcgactggcc gcgctgcgcc tgaagccgga gcgcgaggcc gacatcgtcg acgagatcgc
      361 gcagcacctc gccgaacgct accgcgaggc gacgcaagcc ggcgcgtcgc gcgaggacgc
      421 gacacgcgcg gcgctcgcgg agttccaagc cggcaatgcg ctcgcgcagc gcatcgccgc
      481 gctcaagcaa gcgcacgcgc cggccactgt cacgccgggc gcttcgacgg gccacttgct
      541 cgccgatctc gttcaagacc ttcgctacgc ggcgcgcgcg ttcgcgaaga agccgggctt
      601 cgccgccacg gccgtcctga tcctcgcgct gggcctaggc gcgacgacgg cgatcttcag
      661 cctggtctac ggcgtgttgc tgaagccgct gccgttcccg gagccgcagg cgctcgtgtc
      721 gctgcgccac tacgcgaatt tcggcgagac gagtcaagga ccggccacct atttcaccta
      781 tcgcgagaat caacggtcgt tcgaggacat cggtgcctgg gaccaagcca ccgtcgccat
      841 cacaggcggc ggcgaaacgc ccgaacgcgt cgaagcgctg atcgtgagct catcgacgtt
      901 gcagctgctg cgcgcgcaac cgcggctcgg ccggttcttc ggcacggacg acgacgtgat
      961 cggcagcgcg cctcgagcca tcctgaccgc gggctactgg cagcgccggt tcggcggcgc
     1021 cgaggacatc ctcggccgcg tgatcacgat cgacggcacg cccacggaga tcatcggcgt
     1081 gctgcctccg tccttcgagt tcttgagcac gcggcccggc gtgctgctgc cgatgccgct
     1141 caatccgaac gcgcgctgga tcagcttcgg gttcaatgcc gtcgcgcgcc tgaagcccgg
     1201 cgtgacgccg gagcaggcga gcgccgacgt cgagcacatg atctcgctgc ttccatcgat
     1261 cttcgcgccg ctcgagctgc ggccgaacgt gcgtccgctg gcggacgacg tcacaggcaa
     1321 cgtcgccgac gtgctgtgga tcttgctcgc ggccgtcggc gtcgtgctgc tcatcgcgtg
     1381 cggcaacgtc gcgaacctgt tcctcgtgcg cgcggaggct cggcatcaag agctcgcgac
     1441 gcgcgcggcg ttaggcgcca gccgcggccg cctcgcgcgt acgctgctcg cggagagcgt
     1501 cgcgctcgcg ctcgcgggcg gcgtgctcgg tgttgccctc gcgagcttag ggctagcgct
     1561 gctgcgcgcg atcggcccgg cgcagctgcc gcggctcgag gaagtcggca tcgatccggc
     1621 ggtgctgttg ttcgcagcag cggtttcgat cgtcagcggc gtgctgttcg gaatgttcgc
     1681 cgtcgcgcgc tatggcaacc ccggcatcgc gatgctgaag agcggcagcc gcgcggccag
     1741 tgaatcgcga gaacgcaacc gcgctcgcaa tgcggtcgtc atcggtcaag tcgcgttggc
     1801 tttgacgctg ctgatcgtct cgggcctcat ggctcgcacg ctcgtagcga tgctcggcgt
     1861 ggacccaggc ttcacgcttc cgaagcaagt gcagacgttc gtcgtcgcgg ttccggacac
     1921 gctcattgcc gacgacgagc aagcggcgcg cacgcaccag agcatcgcgg aacgactcca
     1981 gcaagtcccc ggcgtgctct cggtcggcct ttcgtcctcg atcacgatgg acggcgagga
     2041 caacggcaat tacctcacgg ccgacgggct tcccgacaaa gggctcgaag gcccgcagcg
     2101 acgcttcaag agcgtcggcc ccggctattt cgagacgatg ggcaacccga tcgtggcagg
     2161 acgttcggtc gagtggagcg acgtcctcgc gcggcggcac ccggtcgtca tctccgagac
     2221 gctggcgcgc gagctctggc aggagccttc agaggcgatc ggcagccgcg tgcgctgctg
     2281 cggcaacgaa gagtggagcg agatcgtcgg cgtgagcggc gacgagcgcg acgacgggct
     2341 caaccggccg gcgacggcga tcgtgtactg ggcgatgctg aacgacagct atcggtggcg
     2401 cgcgatggct tacgcggtgc ggtcggagcg cgtcggcacg ccgggcttca tgcgcgagct
     2461 cgagcaagcg gtgtggaacg tgaaccccga tctgccgctc gcgtcgattc aaacgctcga
     2521 gcagatccaa acgacgtcga tggcgaagac ctcgttcacg ctcgtgatgc ttgtgatcgc
     2581 cgcgagcgtc gcgttgctgc tcggcgtagt cggcatctac ggcgtgatct cgtacgtcgc
     2641 cgcgcaacgc acgcgcgaga tcgggattcg catcgcgctc ggcgcgaagc tcgccgacgt
     2701 gcggaagcta ttcttgagcc acggcctatg gctcacgggc agcggcatcg cgctcgggct
     2761 cgtgctcgcg gtggtggtga cgcgcgtgat gtcgagctat ctgttcggcg ttgggcccgt
     2821 ggacccgctc acctacgcgg ccgtgtccgc gctgcttgcg gcgatttcgt tgcttgcgat
     2881 ctacctgccg gcgcggcgcg ccgcgcgcat cgaccccaac gtcgcgttgc gcgccgatac
     2941 ctaagagctc gtcgagatca ccggagatcc acatgcacga ctggaaaagc gaagtccgcg
     3001 cgcggctcgc gccattgcgt ttgaagcccg agcgtgaggc cgacatcgtc gacgagatcg
     3061 cgcagcacct cgcggagcgc taccgcgaag cgatctcggg cggcgcatcg catcaagaag
     3121 caacgcgcgt cgcgctcgcg gagtttcaag ccggcaacgc gctcgcccag cgcatcgccg
     3181 cgctcaagca agcgcacgcg ccggcacccg tcacggcggg cgcctcgacg ggacgcctgt
     3241 tcgcggatct caagca
//
LOCUS       DASSNN010000082         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_396740, whole genome shotgun sequence.
ACCESSION   DASSNN010000082 DASSNN010000000
VERSION     DASSNN010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396740"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1488
                     /locus_tag="VFL84_01525"
     CDS             52..1488
                     /locus_tag="VFL84_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7607326.1"
                     /translation="MRLTQALGAGSVAALCAVLLAGAGATQEGDATARLAALEAELAT
                     LEHDVGLLEDTKAIKRLQRAYGYYVDKKLSREIGALFADAPSTTAELGGSGVYVGRAR
                     IAEFYDRIIGGEGLKPGQLFNHMILQGVVHVAPDGRTAKGRWRALIQIGQHGESAVWS
                     EGPYENEYVKESGVWKFSKVHWYMTVAAPYSPGWHKAPQPMEPPLADFPPDRPPTVVY
                     ASYPGVYQPPYHYANPVSGRCEPGVCAVETKAADASARAPAASPVGSVEDARARLAAV
                     QARAVRIDDVNAIQNLQGSYGYYTDKMLWDEVVDLFADDGTIEIGPSGVYAGKDSIRR
                     YLLSLRDGKQGPLESVLNDHFQLQPIVTVADDGLTAKGRWRLFLMTGESGSGSGGNWG
                     EGVYENEYVKDNGVWKIRSLHWYANFIAPYEGGWLNADPKAIESHAMGRGVTPDRPST
                     VSYEPYPGVFVPPFHYVNPVTGQTGARQ"
     gene            1485..>2018
                     /locus_tag="VFL84_01530"
     CDS             1485..>2018
                     /locus_tag="VFL84_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7607327.1"
                     /translation="MSRTTLGLAAVCCALACSACGSRGNDAETLAAIAELETAVADAR
                     REVDRLKDVDELENLASIYGHYVDKSRHDDVADLFAPEGVVEILGRGVFIGQDRVREY
                     MRNLTPGNVGPREGSLFNHMHLQPVVHVSPDGDTAHVRSRLFVMFGIMNTNAQWGDGI
                     YENVFVKQDGVWKLDYLH"
BASE COUNT          352 a          657 c          705 g          304 t
ORIGIN      
        1 ggcaggcccg cccggcgcct agactccggg cgggcacggg gggagaaagc catgcgtctg
       61 acccaggctt tgggcgcggg aagcgttgcg gcgctctgtg ccgtgctgct cgcaggggcc
      121 ggcgcaacgc aagaaggcga cgcgaccgcg cggctcgcgg cgctcgaggc cgagctcgcg
      181 acactcgagc acgacgtcgg cctgctcgag gacaccaaag cgatcaagcg gctgcagcgc
      241 gcgtacggct attacgtcga caagaagctg agccgcgaga tcggcgcgct gtttgcggat
      301 gccccaagca cgaccgcgga gctcggcggc tcgggggtct atgtcgggcg cgcgcgcatc
      361 gcggagttct acgaccgcat catcggcggc gaaggcttga agcccggcca gctcttcaat
      421 cacatgatct tgcagggcgt cgtgcacgtc gcgcccgacg gccgcacggc gaaaggccgc
      481 tggcgcgcgc tgattcagat cggccagcac ggcgagagcg ccgtgtggtc ggaagggccg
      541 tacgagaacg agtacgtcaa ggaaagcggc gtctggaagt tctcgaaggt ccactggtac
      601 atgacggtcg cggcgcctta ctcgccgggc tggcacaaag cgccgcagcc catggagccg
      661 ccgctcgcgg acttcccgcc ggaccggccg cccacggtcg tgtacgcgtc ttaccccggc
      721 gtctatcaac cgccgtatca ctacgcgaac ccggtgtcgg gacgttgcga gcccggcgtc
      781 tgcgccgtcg agacgaaggc cgcggacgct tcggcacgcg cgccggccgc ctcgccggtt
      841 ggcagcgtcg aggacgcgcg cgcgcggctc gccgccgtgc aggcgcgcgc cgttcgcatc
      901 gacgacgtca acgccatcca aaacctgcag ggcagctacg gttactacac cgacaagatg
      961 ctttgggacg aggtcgtcga tctgttcgcg gacgacggca cgatcgagat cggcccgagc
     1021 ggcgtctatg ccggcaagga cagcattcgc cgctatctgc tgagcttgcg cgacggcaag
     1081 caagggccgc tcgaaagcgt gctgaacgat cacttccagc tacagccgat cgtcacggtc
     1141 gccgacgacg gcctcacggc gaagggccgc tggcggctgt tcttgatgac cggcgaatcg
     1201 ggctcgggat cgggcggcaa ctggggcgag ggcgtctacg agaacgagta cgtcaaggat
     1261 aacggcgtct ggaagatccg ctcgctgcac tggtatgcga atttcattgc gccgtatgaa
     1321 ggcggctggc tgaacgcgga cccgaaggct atcgaaagcc acgcgatggg ccgcggcgtg
     1381 acgccggacc ggccgtcgac ggtgtcgtac gagccctacc ccggcgtgtt cgtgccgccg
     1441 ttccactacg tgaatcccgt caccgggcag acgggagcgc gccaatgagc cgaacgacgt
     1501 tgggcttggc cgccgtctgc tgcgctctcg cctgctcggc atgcggctcg cgcggaaacg
     1561 atgccgaaac gctcgcggcc atcgccgagc tcgagacggc cgtcgccgac gcgcggcgcg
     1621 aggtagaccg cttgaaggac gtcgacgagc tcgagaacct cgcgagcatc tacgggcact
     1681 acgtcgacaa atcgcgtcat gacgacgtcg cggatctgtt cgcgccggag ggtgtcgtcg
     1741 agatcttggg ccgcggcgtc ttcatcggtc aggaccgcgt gcgcgagtac atgcgcaacc
     1801 tgacgccggg caacgtgggc ccgcgcgaag ggtcgctgtt caatcacatg cacctgcagc
     1861 ccgtcgtgca cgtctcgccc gacggcgaca cggcgcacgt gcggtcgcgg ctgttcgtga
     1921 tgttcggcat catgaacacg aacgcgcaat ggggcgacgg aatttacgag aacgtcttcg
     1981 tcaagcagga cggcgtctgg aagctcgact atttgcac
//
LOCUS       DASSNN010000083         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1648080, whole genome shotgun sequence.
ACCESSION   DASSNN010000083 DASSNN010000000
VERSION     DASSNN010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648080"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1197
                     /locus_tag="VFL84_01535"
     CDS             217..1197
                     /locus_tag="VFL84_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HET7607328.1"
                     /translation="MTKPPVEARAFWIAEPKRGEIRTEALRAPASGEALVRTLYTGIS
                     RGTEALVFNGKVPLGERERMRAPFQAGEFPAPVKYGYSSVGIVERGPAELVGRRVFCL
                     YPHQTRYVVPVAALHVLPDDVPPARAVLAANVETAVNVLWDAAPRVGDRVAVVGAGTV
                     GCVAAWLAARIVGTEVELVDVDERKAAAARALGVPFRSGTAARRDADVVIHTSGTAAG
                     LATALELAGVEATIVEASWYGDELPAVPLGGAFHSRRLTLKSSQVGAVAAAQRARWSH
                     ARRMELVMRLLAGAELDALVTGESPFEDLPNVLARLAASPGYTLTHRIAY"
     gene            1205..1609
                     /locus_tag="VFL84_01540"
     CDS             1205..1609
                     /locus_tag="VFL84_01540"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET7607329.1"
                     /translation="MFTVSVRDHFMIAHSFEGQVFGPAQKLHGATYVVDVEFRRAALD
                     PDGIVVDIGRATDALRDVLAALNFRNLDDEPAFRGRNTTTEFLASVIHERIAAKIAAG
                     ELGPHASGLASLRVTLHESHVAWAAYEAKLGR"
     gene            1587..>2584
                     /locus_tag="VFL84_01545"
     CDS             1587..>2584
                     /locus_tag="VFL84_01545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7607330.1"
                     /translation="MKPSSAASSRGFDFVVPGDIRTPTGGYIYDREIVAGLTERGFRI
                     AVHSLDASFPSPTPAALRAARATFASLADGRVVVIDGLALPGLDRLLADEAKRLTLVA
                     LVHHPVALETGLDPIQAERYGALERSALGYVQRVITTSQWTARTLAAEGVPVAQLRVV
                     EPGVDRRKTRGSSDPKASAAPGAAQDVLNLLCVGTLTPRKGHAVLLEALNEIRDRHWH
                     LTCAGSLLRDAPTVAAIQHQIDRLSLRKRVSLLGDLDREALERQYARADVFVLPSYLE
                     GYGMALAEAVAFGLPVVSTTAGAIPETVPANAALLVAPGDSRALAKALASLIDDPAR"
BASE COUNT          345 a          963 c          901 g          375 t
ORIGIN      
        1 gagccagctc gcgagccagg ccgcggcgcc aacggttgcc gccgccgcga gcacggccgc
       61 gacggccgcg ccgcgcggcc aagccacgtg ctcgccggcg cggcgctcga tctcgacggc
      121 ggggggaacg gccatgagac gtgacgcttg cacgccggcc gaccggtttg caagtatgcc
      181 gccgcgggcg ccgtgcgcct cgcgggccag ctcgccatga cgaaaccgcc agtcgaagcg
      241 cgcgcattct ggatcgccga gccgaagcgc ggcgagattc ggacggaagc gttgcgcgcg
      301 ccggcgtccg gcgaggcgct cgttcgaacg ctctacaccg gcatcagccg cgggaccgag
      361 gcgctcgtct tcaacggcaa ggtgccgctc ggcgagcgcg agcgcatgcg tgcgcctttc
      421 caggccggcg aatttccggc gccggtcaag tacggctact cgagcgtcgg catcgtcgag
      481 cgagggcccg cggagctcgt cggccggcgc gtgttctgcc tctatcctca tcagacgcgc
      541 tatgtcgtgc ccgtcgcggc gctgcacgtg ctgcccgacg acgtgccgcc cgcgcgcgcc
      601 gtgctcgccg ccaacgtcga gacggccgtc aacgtgctct gggacgcggc gccgcgcgtc
      661 ggcgaccgcg tcgccgtcgt cggcgcgggc acggtcggct gcgtcgccgc ttggctcgcc
      721 gcgcgcatcg tcggcaccga ggtcgagctc gtcgacgtcg acgagcgcaa agccgcggcc
      781 gcgcgcgcgc tcggcgtgcc gttccgctcg ggaaccgcgg cgcgccgcga tgcggacgtc
      841 gtcattcaca cgagcggcac ggccgcgggg ctagcgacgg cgctcgagct cgccggcgtg
      901 gaagcgacga tcgtcgaggc gagctggtac ggcgacgaac tgcccgcggt gccgctcggc
      961 ggcgcgttcc acagccggcg tctcacgctc aagtcgtcgc aggtcggcgc cgtcgccgcg
     1021 gcacagcgcg cgcgctggag ccatgcgcgg cgcatggagc tcgtcatgcg gctcctcgcc
     1081 ggcgcggagc tcgacgcgct cgtgaccggc gaaagcccgt tcgaggatct tccgaacgtg
     1141 ctcgcgcggt tggccgcgtc gcccggttac actttgacgc accgcatcgc ttactgagcg
     1201 caacatgttc accgtttccg tacgcgacca cttcatgatc gcgcacagct tcgagggcca
     1261 ggtcttcggt cccgcccaga agctgcacgg cgcgacatac gtcgtcgacg tcgaattccg
     1321 ccgtgccgcg ctcgaccccg acggcatcgt cgtcgacatc ggccgcgcaa ccgacgcgtt
     1381 gcgcgacgtg ctcgccgctt tgaattttcg caacctcgac gacgagccgg ctttccgcgg
     1441 ccgcaacacg acgacggagt tcctcgcgtc cgtgattcac gagcgcatcg cggcgaagat
     1501 cgcggccggc gagctcggcc ctcacgcgag cgggctcgcg agcttgcgcg tgacgctgca
     1561 cgagtcgcac gtcgcctggg ccgcctatga agccaagctc ggccgctagc tcgcgcggct
     1621 tcgacttcgt cgtgcccggc gacattcgca cgccgacggg cggctacatc tacgaccgcg
     1681 agatcgtcgc cggcctcacg gagcgcggct tccgcatcgc cgtgcactcg ctcgacgcga
     1741 gcttcccttc gccgacgcct gccgcgctgc gcgccgcgcg cgcgacgttc gcttctctcg
     1801 ccgacggccg cgtcgtcgtc atcgacggcc tcgcgttgcc ggggctcgat cggctgctcg
     1861 ccgacgaggc caagcgcctc acgctcgtcg cgctcgtgca tcatcccgtc gcgctcgaga
     1921 cggggctcga cccgatccag gccgagcgct acggcgcgct cgagcgcagc gcgctcggct
     1981 acgtgcagcg cgtgatcacg acgagccaat ggacggcgcg cacgctcgcc gccgaaggcg
     2041 tgcccgttgc gcagctgcgc gtcgtggagc ccggcgtcga tcggcgcaag acgcgcggca
     2101 gctccgaccc gaaagcttcg gcggctcccg gcgcggcgca agacgtgctg aacctgctct
     2161 gcgtcggcac gttgacgccg cgcaagggac acgccgtgct gctcgaggcg ttgaacgaga
     2221 tccgcgaccg ccattggcac ttgacttgcg ccggcagctt gctgcgcgat gcgccgaccg
     2281 tcgccgcgat ccagcatcag atcgaccgcc tgagcttgcg caagcgcgtg agcctgctcg
     2341 gcgatctcga ccgcgaggcg ctcgagcgcc aatacgcgcg agccgacgtg ttcgtgctgc
     2401 cttcgtatct cgagggctat ggcatggcgc tcgccgaggc cgtcgcgttc gggctgcccg
     2461 tcgtgagcac cacggccggc gcaatcccgg agacggtgcc cgcgaacgcg gcgctactcg
     2521 tcgcgccggg cgacagccgc gcgctcgcga aagcgctggc ctccttgatc gacgatccgg
     2581 cacg
//
LOCUS       DASSNN010000084         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_396878, whole genome shotgun sequence.
ACCESSION   DASSNN010000084 DASSNN010000000
VERSION     DASSNN010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396878"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFL84_01550"
     CDS             <1..282
                     /locus_tag="VFL84_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7607331.1"
                     /translation="EARSRFYNAVSLKLSNPELIPAARNAIKLLPEVASTELDEKNGR
                     ITAFPAPGKLPLAPINALAERQRWPLSELHLESGRLDEVFRSITGGATA"
     gene            279..1013
                     /locus_tag="VFL84_01555"
     CDS             279..1013
                     /locus_tag="VFL84_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607332.1"
                     /translation="MSGVFAVFKRELASYFATPIAYVFIVIFLIVSGWFTFFYGGFYE
                     AGQADLGAFFYWHPWLYLLLVPALSMRLWAEERKSGSIELLLTLPLETWQVVAGKYLA
                     AWAFTAISVALTFPIWITVNYLGEPDNGVIFAAYLGSILMAGGFLAVGACLSAATKNQ
                     VIAFILSVVTCFALLVPGLGVVQQVLSGIAPQWLVDGLTSLGPQAHFDYISKGVIDLR
                     DIIYFALLIGMALYANTVVLEIKKAD"
     gene            1018..>2788
                     /locus_tag="VFL84_01560"
     CDS             1018..>2788
                     /locus_tag="VFL84_01560"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HET7607333.1"
                     /translation="MSTTRNKLGYVTLVLVVLAFVVAVVAANAWLRGMRVDLTENNLY
                     TLGEGTQAVLDDIDEPINLYFFFSNDATANLPSLRTYAARVREMLEEFAARAPEGKLV
                     LNIVDPQPFSEEEDRAEQLGLQSAMVGPGGEAVYFGLAGTNSVGTSDRIAIFDPRKES
                     FLEYDIARLVYNLANTDKTVVGLLSSAPVGGGFDPVSQQPAQPWVVVEQAKQVLEVRT
                     LPPSVISIDEDVDVLWIVHPAMLDDGTLYAVDQFIMRGGRALVFVDPNAEILQGPDPS
                     GLGIGGAASTSTLGRLFDAWGVKFDTTKVVADNRYALSIGGRFQPMRHVGLIGIDAEA
                     MSQDDPITSGLGSVNFGVAGHFELAEGAKATLSPLVKSSVESQLMPADRFQFLPDPEE
                     LLNGFTPSGKEYVIAARLSGPLSSAFPDGPPPAQGSEAPVDAALASKHLASTENANLV
                     LVGDVDVLSDRLWVQAQSFLGQRIYNAFANNGDFVINALDNLSGSAALIGLRSRATYT
                     RPFTTVEELRRRADLEFRATEQRLEGELTQTEQRLGELQKSRSDGNSLLMSPEQQAEI
                     QRFLDEQVRIRQELRAVRRNLDAD"
BASE COUNT          455 a          933 c          915 g          485 t
ORIGIN      
        1 gaggcgcgct cgcgcttcta caacgccgtg tcgctgaagc tatcgaatcc cgagctcatc
       61 cccgcggcgc gcaacgcgat caagctgctg cccgaggtcg cgagcaccga gctcgacgag
      121 aagaacgggc ggatcacggc attcccggcg cccggcaagc tgccgctcgc cccgatcaac
      181 gcgctcgccg agcgccaacg ctggccgctc tcggagctgc acttggagtc cggccggttg
      241 gacgaggtgt tccgctcgat cacgggcgga gcgacggcat gagcggcgtc ttcgcggtgt
      301 tcaagcgcga gctcgcgagc tacttcgcga cgccgatcgc gtacgtcttc atcgtgatct
      361 tcctgatcgt ctcgggttgg ttcacgttct tctacggcgg cttctacgag gcggggcagg
      421 cggacttggg cgcattcttc tactggcacc cgtggctcta cctgctgctc gtgccggcgc
      481 tctcgatgcg gctctgggcc gaagagcgaa agtccggaag catcgagctc ttgttgacgc
      541 tgccgctcga gacttggcaa gtcgtcgcgg gcaagtatct ggccgcgtgg gcgttcacgg
      601 cgatctcggt cgcgctgacg ttcccgatct ggatcaccgt caactacctc ggcgagcccg
      661 acaacggcgt gatcttcgcc gcctatttgg gcagcatcct gatggccggc ggcttcctcg
      721 ccgtcggcgc atgcttgtcc gctgcgacga agaaccaggt catcgcgttc atcctgtcgg
      781 tcgtcacgtg ctttgcgctg ctcgtgcccg gcctcggcgt cgtgcagcag gtgctgtccg
      841 ggatcgcgcc gcaatggctc gtcgacgggc tcacgagctt ggggccgcag gcgcacttcg
      901 actacatcag caaaggcgtc atcgatctgc gcgatatcat ctatttcgcg ctgctgatcg
      961 gcatggcgct ttacgcgaac acggtcgtgc tcgagatcaa gaaggcggac tgacgcgatg
     1021 agcactacta ggaacaagct cgggtacgtg acgctggtgc tcgtcgtcct cgcattcgtg
     1081 gtcgccgtcg tcgcggcgaa tgcctggctg cgcggcatgc gcgtcgatct gaccgagaac
     1141 aacctctaca cgctcggcga gggcacgcag gcggtcctcg acgacatcga cgagccgatc
     1201 aatctctatt tcttcttctc gaacgacgcg acggcgaact tgccgagcct gcgtacctac
     1261 gccgcgcgag tgcgggagat gctcgaggaa ttcgcggcgc gcgctcccga gggcaagctc
     1321 gtcttgaaca tcgtcgatcc gcagccgttc tcggaggaag aggatcgcgc ggagcagctc
     1381 gggctccagt cggccatggt cggtcccggc ggtgaggccg tctatttcgg gctcgcgggc
     1441 accaacagcg tcggcacgtc ggatcgaatc gcgatcttcg acccgcgcaa ggagtcgttc
     1501 ctcgagtacg acatcgcgcg cctcgtctac aacctcgcga acaccgacaa gaccgtcgtc
     1561 ggcttgctgt cgagcgcgcc ggtcggcggc ggattcgatc ccgtctcgca gcagccggcg
     1621 caaccgtggg tcgtcgtcga gcaagcgaag caggtgctcg aggtgcgcac gttgcccccg
     1681 agcgtgatct cgatcgacga ggacgtcgac gtgctctgga tcgtgcatcc cgcgatgctc
     1741 gacgacggca cgctctacgc cgtcgaccag ttcatcatgc gcggcgggcg cgcgctcgta
     1801 ttcgtcgacc cgaacgccga gatactgcaa ggacccgacc cgtcgggcct cgggatcggc
     1861 ggcgcggcat cgacctcgac gctggggcgc ctcttcgacg cctggggcgt caagttcgac
     1921 acgacgaagg tcgtcgccga caaccgttac gcgctcagca tcggcggtcg atttcaaccg
     1981 atgcgtcacg tcgggctgat cgggatcgac gccgaggcga tgagccagga cgacccgatc
     2041 acgtcgggct tgggctcggt caacttcggc gtcgcgggac acttcgagct cgccgaaggg
     2101 gccaaagcga cgttgtcgcc gctggtcaag tcgagcgtcg agtcgcagct catgccggcc
     2161 gaccggtttc agttcttgcc ggatcccgag gagctcttga atggcttcac gccgagcggc
     2221 aaggaatacg tcatcgccgc gcgcctctcg ggcccgctga gctcggcttt ccccgacggc
     2281 ccgccgcccg cgcagggaag cgaggccccc gtcgatgccg cgctcgcaag caagcatctc
     2341 gcctcgaccg agaacgcgaa cctcgtgctc gtcggcgacg tcgacgtctt gagcgaccgg
     2401 ctctgggtgc aggcgcagag cttcctcgga cagcgcatct acaacgcctt cgcgaacaac
     2461 ggcgacttcg tgatcaacgc gctcgacaat ctctcgggca gcgccgcgct gatcggcttg
     2521 cgcagccgcg cgacgtacac gcggccgttc acgaccgtcg aggagctgcg ccgccgcgcc
     2581 gacctcgagt tccgcgcgac ggagcagagg ctcgagggcg agctcacgca aacggagcag
     2641 cggctgggcg agctccagaa gtcgcgcagc gacggcaact cgctgctgat gagcccggag
     2701 cagcaagcgg agattcagcg cttcctcgac gagcaagtgc ggatccgcca ggagctgcgc
     2761 gccgtgcgcc gcaacctcga tgcggaca
//
LOCUS       DASSNN010000085         5304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1648130, whole genome shotgun sequence.
ACCESSION   DASSNN010000085 DASSNN010000000
VERSION     DASSNN010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648130"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VFL84_01565"
     CDS             complement(<1..1200)
                     /locus_tag="VFL84_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET7607334.1"
                     /translation="MTAASPLSPDIERLLRGRLHEPRQVLGVHASSEQDVVVRVLLPN
                     ALRVRLVEPAVELERVAGTALFEWKGPRDGLTTPYRLRFESNDGRWHEIYDPYSFPLE
                     IDEHDLARFAGGTHIHAHRFLGAHERTIAGVRGIRFAVWAPNAERVSVVGTFNHWDGR
                     YHPMSVRGSSGVWELFVPEIGAGELYKYEVFTRDSRELKLKSDPYAAAFEERPATAGV
                     TTAPSTFGWDDAAWLMQRARRDWLKEPMSVYEVHLGSWRRNAAGNFLNYRDIAAQLAA
                     YVKDLAFTHVELMPITEHPFDDSWGYQCTGYFAPTSRHGSPDDLRAFVAELHRNGIGV
                     LLDWVPGHFPKDDHALARFDGTALYEYGDWQKGEHPDWSTLVFNYTRHEVQSFLLSSA
                     ICWLDDFH"
     gene            1278..2543
                     /gene="glgC"
                     /locus_tag="VFL84_01570"
     CDS             1278..2543
                     /gene="glgC"
                     /locus_tag="VFL84_01570"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET7607335.1"
                     /translation="MRQIPRRRLVSQLTRSTVAVVMAGGRGQRLMQLTDQRAKPATPF
                     GGKYRIIDFSLSNCVNSGIRQIFILTQYKAHSLIQHVQRGWGYLHGELGEFIDIVPAQ
                     QQLGEIWYRGTADAVYQNLDIIHQHRPETVLILAGDHVYKMDYGPLIAFHKESGADIT
                     IGGVQVPVAQAHEFGVMTLGSDHRVISFDEKPKQPRPMPGRESVALASMGIYVINPEF
                     LREALERDAAVPESQHDFGRNIVPEAVTNRRVFAYPFEDVETKAQRYWRDVGTVDAYY
                     NANMELIHVSPELNLYDQAWPIWTYQQQVPGAKFILDEDNRRGLAINSMIAGGCIVSG
                     AVVRESMLFFSVFVDEGTEVRRSVVLPEVEIGRSCYIANAIIDEGCSIPHGTIIGRDR
                     DFDREHFHVTEQGIVLVTRRMLAGLRRAN"
     gene            2552..4018
                     /gene="malQ"
                     /locus_tag="VFL84_01575"
     CDS             2552..4018
                     /gene="malQ"
                     /locus_tag="VFL84_01575"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET7607336.1"
                     /translation="MNLFAERRAGVLLHPTSLPGKGPRGTFGADARHFVDWLVAGAFS
                     VWQTLPLGVTDAHGSPYALRSAHAGDPRFIDAEDLAAVAELPAGIDAAGDREARYASF
                     TALATAEQQRAFAQFTRAHRRWLEPYALFEHLSRRFEQAPWWEWPTPYRDRDAAALRE
                     CRAASRVPLRALVFEQYLFELQWSALKRYANQRGVYLFGDLPFYVDRNNVDVWWERHL
                     FAVDASGHPLAVAGVPPDYFNADGQLWGNPLYDWGAMQRSGFRWWLERIGTQLARFDL
                     LRIDHFRALESYWAIPAEAPSARLGEWQPCPGDALLTALRAEMGGEIPLVAEDLGIIT
                     DEVRALRDRFGIPGMAVLQFAFDGSPDNPYLPEHHRRNSVVYTGTHDNDTTVGWYASL
                     DSRAREIVHETLGVGPAPPVPDFLIDAAYASRAALAIIPMQDLLARDSGARMNTPGTV
                     VGNWRWRFEWADVPSSVAAVSLDRAVRSGRAPRGAAQR"
     gene            complement(4216..>5304)
                     /locus_tag="VFL84_01580"
     CDS             complement(4216..>5304)
                     /locus_tag="VFL84_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HET7607337.1"
                     /translation="RLSAAAVALSAADGWRPDVLHCHDWHTALVPVFQRLPPRSPARS
                     VLTLHNVGYQGVFAAAVFAEHGFAALEQVLPADARTGSDTNFLRAGLRAADRVTTVSP
                     TYAEEIRRPEFGMGLEDVLRERGGDLVGILNGVDYSVWSPERDPFLPYRYDAKDLAPK
                     RLLKKRLVERLKLVVDVDAPLVGVVSRLVPQKGIDLLAAVMPALLQDTSASFAVLGNG
                     DAAIAAELRALVDAYRTRVSFIEGYDEALAHEIFAGSDLTVVPSRYEPCGLTQMYALR
                     YGSIPVVRATGGLADTIRHFDPSTGLGNGSVFNDADPQGVSWGVRTALRWYADPDLWS
                     QLVRNAMAADFSWPRQIPHYEELYRSML"
BASE COUNT          836 a         1781 c         1820 g          867 t
ORIGIN      
        1 gtggaaatca tcgagccagc agatcgcgct cgacaacagg aagctctgca cctcgtggcg
       61 cgtgtagttg aatacgagcg tgctccaatc ggggtgctcg cctttctgcc agtcgccgta
      121 ctcgtagagg gccgtgccgt cgaagcgggc gagagcgtgg tcgtccttcg ggaaatggcc
      181 cggcacccaa tcgagcagca caccgatgcc gtttcggtgc agctccgcga cgaaggcgcg
      241 caggtcgtcg ggcgagccgt ggcggctcgt cggcgcgaag tagcccgtgc actgatagcc
      301 ccacgagtcg tcgaacgggt gctccgtgat cggcatcagc tcgacgtgcg tgaacgccaa
      361 gtccttgacg tacgccgcga gctgcgccgc gatgtcgcgg tagttcaaga agttgccggc
      421 cgcattgcgg cgccacgacc cgaggtgcac ctcgtagacc gacatcggct ccttgagcca
      481 gtcgcggcgc gcgcgctgca tgagccacgc ggcgtcgtcc cagccgaacg tcgacggcgc
      541 cgtcgtgacg ccggccgtcg cgggccgctc ctcgaacgcc gcggcgtacg ggtccgactt
      601 cagcttcagc tcacggctgt cgcgcgtgaa gacctcgtac ttgtagagct cgccggcgcc
      661 gatctcgggc acgaacagct cccacactcc cgagctgccg cgcacgctca tcgggtggta
      721 gcggccgtcc cagtggttga acgtgccgac gacgctgacg cgctcggcat tcggcgccca
      781 gaccgcgaat cgaatgccgc gcacgccggc aatcgtccgc tcatgggcgc cgaggaagcg
      841 atgcgcgtgg atgtgggtac cgcccgcgaa gcgggcgagg tcgtgctcgt cgatctcgag
      901 cgggaacgaa tacgggtcgt agatttcgtg ccagcggccg tcgttcgatt cgaaacgcaa
      961 tcgatacggc gttgtcaggc cgtctctggg tcccttccat tcgaacagcg ccgtgccggc
     1021 gacgcgctcg agctcgacgg ccggctcgac gagcctgacg cgcaaagcgt tcggcaacag
     1081 cacgcgcacg acgacgtcct gctcgctcga ggcgtgcacc ccgagcactt ggcgcggttc
     1141 atgcagccgg ccgcggagca ggcgctcgat gtcgggcgag agcggcgaag cggctgtcat
     1201 cacgcggttc gtcggcggtg gcattcgtgc ggttctcggt tagacaggcg gagtccttcg
     1261 gtagtagcgt gtagcgaatg aggcaaattc cgcgccgtcg tctcgtcagt cagctcaccc
     1321 gcagcaccgt cgccgtcgtc atggcgggcg gccgcggtca acgtctgatg caactcacgg
     1381 accaacgcgc caagcccgcc actccgttcg gcggcaagta ccgcatcatc gatttttcgc
     1441 tgtcgaactg cgtcaactcc ggaatccgtc agatcttcat cctcacgcag tataaggcgc
     1501 actccctgat ccagcatgtg cagcgcggct ggggctactt gcacggcgag ctcggcgagt
     1561 tcatcgacat cgtgccggcc cagcagcagc tcggcgaaat ctggtaccgc ggcacggccg
     1621 acgccgttta ccaaaacctc gacatcattc atcaacatcg tccggagacg gtcctcattc
     1681 tcgccggcga tcacgtctat aagatggact acggaccgtt gatcgcgttc cacaaggaaa
     1741 gcggcgcgga catcacgatc ggcggcgtgc aggtgccggt cgctcaagcg cacgagttcg
     1801 gcgtcatgac gctcggctcg gatcatcgcg tgatcagctt cgacgagaag ccgaagcagc
     1861 cgcggccgat gccggggcgc gagagcgtcg cgctcgcgtc gatgggcatc tacgtcatca
     1921 acccggagtt cctgcgcgaa gcgctcgagc gcgacgccgc cgtcccggaa tcgcagcacg
     1981 acttcggccg caacatcgtg cccgaggccg tcacgaaccg gcgcgtgttc gcgtatccgt
     2041 tcgaggacgt cgagacgaag gcgcagcgct attggcgcga cgtcggaacg gtcgacgcct
     2101 attacaacgc gaacatggag ctgatccacg tgagccccga gctgaacctc tacgaccagg
     2161 cgtggccgat ctggacgtat cagcagcagg tgccgggcgc gaagttcatt ctcgacgagg
     2221 acaaccggcg cggcctcgcg atcaactcga tgatcgcggg cggctgcatc gtctcgggcg
     2281 ccgtcgtgcg agagtcgatg ctgttcttca gcgtgttcgt cgacgagggc acggaggtgc
     2341 gacgctcggt cgtgctgccc gaggtcgaga tcggccgcag ctgctacatc gcgaatgcga
     2401 tcatcgacga agggtgcagc attccgcacg gcacgatcat cggccgcgac cgcgacttcg
     2461 accgcgagca ctttcatgtc acggagcaag gcatcgttct cgtgacgcgg cgaatgttgg
     2521 cgggcttgcg ccgcgccaac tagcggttgt catgaaccta ttcgccgagc gccgcgccgg
     2581 cgtcctgctc catccgacct cgctgccggg caaagggccg cgcggcactt tcggcgccga
     2641 cgctcggcac ttcgtcgatt ggctcgtcgc cggcgcgttc agcgtttggc agacgctgcc
     2701 gctcggcgtc acggacgcgc acggctcgcc gtacgcgctg cgctcggcgc acgcgggcga
     2761 cccgcgcttc atcgacgccg aggaccttgc ggcggtcgcg gagcttcccg ctggcatcga
     2821 cgcggccggt gatcgcgagg cgcgctacgc ttcgttcacg gcgctcgcga cggcggagca
     2881 gcagcgcgcg ttcgcgcaat tcacgcgggc gcatcgccgt tggctcgagc cgtatgcgtt
     2941 gttcgagcac ttgagccgcc gcttcgaaca ggcgccgtgg tgggagtggc ctacgccgta
     3001 ccgcgatcgc gatgcggcgg cgctgcgcga gtgccgcgcc gcgagccgcg ttccgcttcg
     3061 cgcgctcgtg ttcgagcaat atctgttcga gctccagtgg tccgcgctga agcgttacgc
     3121 gaaccagcgc ggcgtctacc tcttcggcga cctgccgttc tatgtcgatc gcaacaacgt
     3181 cgacgtctgg tgggagcgcc atctattcgc ggtcgacgca agcggccacc cgctggcggt
     3241 agcgggcgtg ccgccggact atttcaacgc cgacggccag ctctggggca atccgctcta
     3301 cgactggggc gcgatgcagc ggagcggttt ccgttggtgg ctcgagcgca tcggcactca
     3361 gctcgcgcgt ttcgatctgt tgcgcatcga tcacttccgc gcgctcgaat cgtattgggc
     3421 gattccggcc gaagccccgt ccgcgcgcct cggcgaatgg cagccctgtc caggcgacgc
     3481 gctgctgacg gcgctgcgcg cggagatggg aggcgagatc ccgctcgttg cggaagactt
     3541 gggcatcatc acggacgagg ttcgcgcgct gcgcgaccgg ttcgggattc cgggcatggc
     3601 ggtgctgcag ttcgcgttcg acggctcgcc ggacaacccc tatcttcccg agcaccatcg
     3661 gcgcaactcg gtcgtctaca cggggacgca cgacaacgac acgacggtcg gctggtatgc
     3721 ctcgctcgat tcgcgcgcgc gcgagatcgt gcacgagacg ctgggcgtgg gtccggcgcc
     3781 gccggtgccg gacttcctga tcgacgcggc gtacgcgtcg agggccgcgc tcgcgatcat
     3841 accgatgcag gacctgctcg cgcgcgactc gggcgcgcgc atgaatacgc cgggcaccgt
     3901 cgttggcaac tggcgctggc gattcgagtg ggccgacgtg ccttcgtcgg tcgctgccgt
     3961 ttcgctggac cgtgctgttc gaagcgggcg tgctccccgg ggcgcggcgc agcggtgaac
     4021 tgtgtcgcgc ggtgcggcgc cgccgcgccg aggctggatc ggtgtggcgt cgatgtgaga
     4081 gggggcgctt ccgagagcgt ctgagcacag ggatgtgcga agacgagccg cagggacgct
     4141 attccggcgt ctctcggaag cgccccctct cgcagcgacg ccacaccgcg cgatcgctcg
     4201 cggcgccgcc tccggtcaaa gcatcgagcg gtagagctct tcgtagtgcg ggatttgccg
     4261 tggccacgag aagtcggcgg ccatcgcgtt gcggacgagc tgcgaccaga ggtcgggatc
     4321 ggcgtaccag cgaagcgccg tgcggacgcc ccacgacacg ccttgcgggt cggcgtcgtt
     4381 gaatacgctg ccgttgccta agccggtcga gggatcgaag tgccggatcg tgtcggcgag
     4441 gcccccggtc gcgcgcacca ccgggatcga gccgtagcgc agcgcgtaca tctgcgtcag
     4501 gccgcacggc tcgtagcgcg acggcacgac cgtcaggtcg gagccggcga agatctcgtg
     4561 cgcgagcgcc tcgtcgtagc cttcgatgaa cgacacgcgc gtgcgatacg cgtcgacgag
     4621 cgcgcgcagc tcggccgcga tcgcggcgtc gccgttgccg agcaccgcga agctcgcgct
     4681 cgtgtcctgc agcagcgcgg gcatgacggc cgcgagcagg tcgatgccct tctgcgggac
     4741 gaggcggctc acgacgccga cgagcggggc gtcgacgtcc accacgagct tcagacgctc
     4801 gaccagccgc ttcttgagga ggcgtttcgg ggccagatcc ttcgcatcgt agcggtacgg
     4861 cagaaacgga tcgcgctccg gcgaccagac gctgtagtcg acgccgttca ggattccgac
     4921 gagatcgccg ccgcgctcgc gcagcacgtc ctcgagaccc atgccgaatt ccggacgccg
     4981 aatctcctcc gcatacgtcg ggctgaccgt cgtcacacga tcggccgcgc gcagccccgc
     5041 gcgcaggaag ttcgtgtcgc tgcccgtgcg cgcatcggcc ggcagcactt gctcgagcgc
     5101 cgcgaaccca tgctccgcga acaccgccgc cgcgaagact ccttggtagc cgacgttgtg
     5161 cagcgtcagc acgctgcggg cagggctgcg cggcggcagg cgctgaaaca cgggcacgag
     5221 cgcggtatgc cagtcgtggc agtgcagcac gtcggggcgc caaccgtcgg ccgcgctcaa
     5281 ggcgaccgcg gcggccgaga ggcg
//
LOCUS       DASSNN010000086         2698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_145562, whole genome shotgun sequence.
ACCESSION   DASSNN010000086 DASSNN010000000
VERSION     DASSNN010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145562"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..2571)
                     /locus_tag="VFL84_01585"
     CDS             complement(187..2571)
                     /locus_tag="VFL84_01585"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET7607338.1"
                     /translation="MTRGCATLLNRGLLAAFAAAALAGCSGEPSPEALLAQGKKLWPL
                     VERYCVDCHNDADLAGDIAFDRLGPGDVAAHADIFERAVRKLRGRMMPPPGESRPGIA
                     ETDAFVAWLEATLDAASPTPEPGYVTPHRMNRTEYANAIRDLLALDVDPATLLPVDGA
                     ERGFDNIANALTVSPSFIDQFLSAARSLSAQAVGNPSPRAVGVPYTIGNARAQQFHVD
                     GLPLGTRGGGVIEHYFPADGEYKLNIGDLVTGVWWLNVEHENTVVALLDGRKFFEVTI
                     GGGQDSKELDQIRAPAVDAINARLKNIPFTATAGVHRLGVTFLHRSFAESDRQLYSQV
                     PGGGQDSVLTLSQLEIFGPVNATGLSSTPSRDKIFVCRPEVSAGERPCAEKIVASLAQ
                     QAFRGAFGSDDLPRLMRLYDEGAASGGFEKGVEHALAGVLAHPKFLYRFEPRPEGIAP
                     GGSYPLDSIELASRLSFFLWSSIPDAELLELAAADKLAEPDVLRAQVERMLADPRAET
                     LASNFAYQWLGLGELDSLAPDPFLFADVDRDIRKYFVTESRLFVDSIFRSDVSVLDLL
                     TARHTFVNEALARHYGIDDVYGTRFRRVELEDQNRFGLLGKGGVLLVSSYPNRTSPVL
                     RGQWLLENVLGVPPHAPPPNVEALVETVEGQKATTVRARLEAHRTNASCNACHAIIDP
                     LGFALENFDAVGRWRTLDREAREPIDASGVLADGTAVSGPKELRDALLERPDQFVQTL
                     TEKLMTYGLGRGLEHTDMPTVRRIVRDAAENDYRFSEIVWGIVNSTQFRMKGGA"
BASE COUNT          438 a          893 c          918 g          449 t
ORIGIN      
        1 acggcacggt cgtgacgggc cccatcgagc tgcgcgaggc gctgctcgag cgcccggacc
       61 agctcgtgca aactttgacc gagaaattaa tgacttacgg gctcggccgc gcgctcacgc
      121 acaccgacat gccgacggtg cggcgcgaca gatgtttcct cgcgatgaac atgcgcgctg
      181 ccccctttag gcgccgccct tcatccgaaa ctgcgtgcta ttcacgatac cccaaacgat
      241 ctccgaaaac cggtagtcgt tctccgcggc gtcgcgcacg atgcgccgga ccgtcggcat
      301 gtcggtatgt tcgagcccgc gcccgagccc gtaagtcatt aatttctcgg tcaaagtctg
      361 cacgaattgg tcgggcctct cgagcagcgc atcgcgcagc tccttaggcc ccgatacggc
      421 cgtgccgtct gcgagcacgc ccgaggcgtc gatcggctca cgggcttcgc gatccagagt
      481 gcgccagcgc ccgacggcgt cgaaattctc gagcgcgaag ccgagcggat cgatgatggc
      541 gtggcacgcg ttgcacgacg cattcgtgcg atgcgcctcg agccgcgcgc gcacggtcgt
      601 cgccttctgc ccttcgacgg tctcgacgag cgcctcgaca ttcggcggcg gcgcatgcgg
      661 cgggacccca agcacgttct cgagcagcca ttggccgcgc agcacgggag aggttctgtt
      721 cggatacgac gacacgagca gcacgccgcc tttgccgagc aagccgaacc ggttctgatc
      781 ttcgagctcg acgcggcgga agcgcgtgcc gtagacgtcg tcgatgccgt agtggcgcgc
      841 gagcgcctcg ttgacgaacg tgtgccgcgc cgtcagcaag tcgagcacgc tgacgtccga
      901 acggaaaatg ctgtcgacga agaggcgcga ctcggtcacg aaatatttgc gaatgtcgcg
      961 atcgacgtcg gcgaacagga acgggtccgg cgccaagctg tcgagctcgc ccaggccgag
     1021 ccattgatac gcgaagttcg acgcgagcgt ctcggcgcgc ggatccgcga gcatgcgctc
     1081 gacttgtgcg cgcagaacgt cgggctccgc gagcttgtcg gccgccgcga gctcgagcag
     1141 ctcggcgtcg gggatcgagc tccataagaa gaaggagagc cgcgacgcga gctcgatgct
     1201 gtcgagcgga tacgagccgc cgggcgcgat gccttcgggt cgaggctcga accgatacag
     1261 gaacttcgga tgcgcgagca cgcccgcgag cgcgtgctcg acgcctttct cgaatccgcc
     1321 gctcgcggcg ccttcgtcgt agaggcgcat caggcgcggc agatcgtccg agccgaacgc
     1381 gccgcggaac gcctgctgcg cgagcgacgc gacgattttc tcggcgcacg gcctctcgcc
     1441 cgccgacact tcgggccggc agacgaagat cttgtcgcgg ctcggcgtgc tcgagagtcc
     1501 cgtcgcgttc acgggcccga agatctcgag ctggctcagc gtcagcacgg agtcttggcc
     1561 gccgcccggc acctgcgagt agagctgccg gtcggactcc gcgaacgagc gatgcaggaa
     1621 cgtgacgccg agccgatgca ctcccgcggt cgccgtgaac ggaatgtttt tgaggcgcgc
     1681 gttgatcgcg tcgacggccg gcgcgcggat ctggtcgagc tccttcgaat cttggccgcc
     1741 gccgatcgtg acctcgaaga acttacggcc gtcgagcagc gcgacgaccg tgttctcgtg
     1801 ctctacgttg agccaccaga ccccggtgac gagatcgccg atgttgagct tgtactcgcc
     1861 gtcggccggg aaatagtgct cgatgacgcc gccgccgcgc gtgccgagcg gcaggccgtc
     1921 gacgtggaat tgctgcgcgc gcgcgttgcc gatcgtgtac ggcacgccga cggcgcgtgg
     1981 gctgggattg cccacggcct gcgcgctcaa gctgcgcgcc gcgctcagga actgatcgat
     2041 gaacgacggc gacaccgtca gcgcgtttgc gatgttgtcg aagccgcgct cggcgccgtc
     2101 gacgggcaag agcgtcgcgg ggtcgacgtc gagcgcgagc agatcgcgga tcgcgttcgc
     2161 gtactcggtg cgattcatgc gatggggggt cacgtagccg ggctcgggtg ttggcgatgc
     2221 ggcatcgagc gtcgcctcga gccacgcgac gaatgcgtcg gtctcggcaa ttccgggtcg
     2281 cgattcgccg ggcggcggca tcatgcggcc gcgcagcttg cgcacggcgc gttcgaatat
     2341 gtcggcatgc gccgcgacgt cgccggggcc gagcctgtcg aatgcgatgt cgcccgcgag
     2401 gtccgcgtcg ttgtggcagt cgacgcagta ccgctcgacg agcggccaca gtttcttccc
     2461 ttgcgcgagc aacgcctcgg gcgacggctc gccgctgcaa cctgcgagtg cagcggctgc
     2521 gaaagcggca agcagcccgc ggttaagcaa tgttgcgcaa ccgcgtgtca tgcgcgtctc
     2581 tcagatggtt ccaggtgctt tgcagcgcca gcgtcacgaa ttgcgtcgga tcgccgtact
     2641 ccgcgtcgaa ctcggccgtc ggtttcgtcg ccaacacctc gtcgacgccg agcgcctt
//
LOCUS       DASSNN010000087         3019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1405542, whole genome shotgun sequence.
ACCESSION   DASSNN010000087 DASSNN010000000
VERSION     DASSNN010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405542"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1494)
                     /gene="hmgA"
                     /locus_tag="VFL84_01590"
     CDS             complement(202..1494)
                     /gene="hmgA"
                     /locus_tag="VFL84_01590"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HET7607339.1"
                     /translation="MSNDPLRYQSGFGNDFASEALPGALPVGQSNPQRPKYGLYTEEI
                     NGTSFTAPRASSRRCWTYRIRPSAVHEPFREISAGLIRSAPFDEAPASPNQLRWRPLP
                     IPQKPTDFVAGLVTMGGNGDPAAQSGTAVHMYAANASMTDRFFYDADGELLLVPQEGA
                     LTVRTELGILEVAPGEICVVPRGVKFRVELAGFARGYVCENYGAHFRLPELGPIGTSG
                     LANSRDFLAPVAAFEERDGDFRVVAKFLGKLWEAKIDHSPLDIVAWHGTYVPYKYDLK
                     RFNTINTVSYDHPDPSIFTVLTAPTATPGTANVDFGVIPDRWSVAENTFRPPPFHRNV
                     CSEFVGLVKGRYIGKAEGFEPGCASLHNCMSGHGPDNDAFEAGSRAENAPQYFADTLT
                     IIFETQLVIKPTRFALETELLERDYYKHWQGLKKNFRR"
     gene            1585..2556
                     /locus_tag="VFL84_01595"
     CDS             1585..2556
                     /locus_tag="VFL84_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HET7607340.1"
                     /translation="MRAIRVHAYGGPEVLKYDEVPEPKPQAGEALVEIAAAGINFIDV
                     QHREGRYKGPSLPFIVGSEAAGTVRAVGAGVTEVAVGDRVAYAMVVGSYAEYAVVPAK
                     RLVKVPAAIDFQTAAAVMLQGLTAHYLTHSTYAIKPGDTALVHAAAGGAGQLVTQVAR
                     LRGATVYGTAGGEEKCAVARAAGAAAAIDYRKQDFEAEIKKLTNGRGVDVVYDSVGKD
                     TFDKSLNCLRPRGLLALFGFSSGPVAPFDPAILGAKGSLFLTRPGLNQYIATREELVA
                     RATDLFTWLATAKLKVTIDRVRPLAEAAEAHRDLEARRTAGKLLLVP"
BASE COUNT          509 a          987 c         1010 g          513 t
ORIGIN      
        1 tattccggcg cctgcgcagc gagcgcggcc tcgatgcctc cgcccgccac gcgctgaccg
       61 acctgattgc cgttctcctc caaaaagggg acagatctat ttttgacgga aaataaatct
      121 gtccccattt tgaaaaaggg gacggatcta tttttcgaga gaaataaatc tgtccccatt
      181 ttgggttgtc ccccttttgg gttagcggcg gaagttcttc tttaggcctt gccagtgctt
      241 gtagtagtcg cgctcgagca gctcggtctc gagcgcgaag cgcgtcggct tgatgacgag
      301 ctgcgtctcg aaaatgatcg tcagcgtatc ggcgaagtat tgcggcgcgt tttccgcgcg
      361 tgagcccgct tcgaacgcgt cgttgtcggg cccgtggccc gacatgcagt tgtgcaggct
      421 cgcgcagccc ggctcgaagc cttcggcctt gccgatgtag cggcctttga cgaggccgac
      481 gaactcgctg cagacgttgc gatgaaacgg cggcggccgg aaagtgttct cggcgacgga
      541 ccagcggtcc gggatcacgc cgaagtcgac gttcgcggtg cccggagtcg cggtcggcgc
      601 cgtcagcacc gtgaagatcg acgggtccgg atggtcgtag ctgaccgtgt tgatcgtgtt
      661 gaagcgctta agatcgtact tgtacggcac gtacgtgccg tgccacgcga cgatgtcgag
      721 cggcgagtgg tcgatcttcg cttcccagag cttgcccaaa aatttcgcga cgacgcgaaa
      781 atcgccgtcg cgctcctcga acgcggccac gggcgccaag aagtcgcgcg agttcgcgag
      841 gccgctcgtg ccgatcggcc cgagctcggg caagcggaag tgcgcgccgt agttctcgca
      901 gacgtagccg cgtgcgaatc cggcgagctc gacgcggaac ttcacgccgc gcggcacgac
      961 gcagatctcg cccggcgcga cctcgagaat gccgagctcc gtgcgcaccg tcagcgcgcc
     1021 ttcctgcggc actagcaaga gctcgccgtc ggcgtcgtag aagaagcggt ccgtcatcga
     1081 cgcgttcgcg gcgtacatgt gcacagcagt gccggactgc gcggccgggt cgccgttgcc
     1141 gcccatcgtg acgagccccg cgacgaagtc ggtcggcttt tgcggaatcg gcagcggccg
     1201 ccagcgcagt tgattcggcg acgccggcgc ctcgtcgaac ggcgcgctgc ggatgagccc
     1261 cgccgagatc tcgcgaaatg gctcgtgcac ggccgacggc cgtattcgat aagtccagca
     1321 gcgccggctc gaggcgcgcg gcgccgtgaa gctcgtgccg ttgatctcct ccgtgtagag
     1381 cccgtacttc ggccgctgcg gattgctctg cccgacgggc agcgcgcccg gcagcgcctc
     1441 gctcgcgaag tcgttgccga acccggactg atagcgaagc ggatcgttgc tcatgcgagt
     1501 cgcgcggtcg aagtcacgcg gggaatagta aaccggcgtc gcggccgcgt gcgatatagt
     1561 tcgatcattc attagggagc cctcatgaga gcgattcgtg tacacgcgta cggcggaccg
     1621 gaagtcctga agtacgacga ggtgccggag ccgaagccgc aggccggcga agcgctcgtc
     1681 gagatcgcgg ccgccggcat caacttcatc gacgtgcagc accgcgaggg gcgctacaag
     1741 gggccgtcgc tgccgttcat cgtgggctcc gaggccgcag gcaccgtcag ggccgtgggg
     1801 gccggagtca ccgaagtcgc cgtcggcgat cgcgtggcct acgcgatggt cgtcggctcg
     1861 tatgccgaat acgccgtcgt gccggcgaag cgcctggtca aggtgccggc cgcgatcgat
     1921 ttccaaacgg ccgccgccgt catgctgcaa ggcttgaccg cgcactacct gacgcacagc
     1981 acctacgcga tcaagcccgg cgacaccgcg ctcgtgcacg cggcggccgg cggagcgggg
     2041 cagctcgtca cgcaggtcgc gcgcctgcgc ggcgcgaccg tgtacggcac cgcaggcggc
     2101 gaagagaaat gcgccgtggc gcgcgcagcc ggtgccgctg cggcgatcga ctatcgcaag
     2161 caggatttcg aagccgagat caaaaagctc acgaacggcc gcggcgtcga cgtcgtctac
     2221 gactccgtcg gcaaggacac gttcgacaag agcctcaact gcttgcgccc gcgcggcctg
     2281 ctcgcgctgt tcgggttctc gagcggcccc gtcgcgccgt tcgatccggc catcctcgga
     2341 gcgaagggct cgttgttcct gacgcgcccc ggcttgaatc agtacatcgc gacgcgcgag
     2401 gagctcgtcg cgcgcgcgac cgatctcttc acgtggctcg ctacggctaa gctgaaggtc
     2461 acgatcgatc gcgtgcggcc gctcgccgaa gcggccgagg cgcatcgcga cctcgaggcg
     2521 cgccgcaccg cggggaagct gttactcgtt ccgtgaagag gacgcggcgc ccttgccgca
     2581 acgctcgcta attgagcgca tgcgcacgcg ccggcgggag ctcgagatcc actcgtatcc
     2641 agcgaccctg cgtcttgacc atcatcacgc ctgtgcgcgg tcgaggcgtc gggatgagct
     2701 cgagtcggtc cgttccgggc acggcaacgc cgatgggttc cttcagcacg atgccggccg
     2761 tgtccgaaat cggaagccaa ctcgcctcgt cgactcgggg tggccgatcc gcagccgact
     2821 gggcggtgga cgccgccact atcaggcaag ccgtcgcggc gacaaaccac tgcgatcgag
     2881 taccgctcat agcgagccct cccatgggtg tcgcgagaac tagacctcgc cgcattcgac
     2941 gggttcaatt cgaagcatct cgccccggct acgacgtgac ggtattcgcg ccgacgtgtg
     3001 cggcttcgaa acgcagagc
//
LOCUS       DASSNN010000088         3644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1405959, whole genome shotgun sequence.
ACCESSION   DASSNN010000088 DASSNN010000000
VERSION     DASSNN010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405959"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFL84_01600"
     CDS             <1..351
                     /locus_tag="VFL84_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HET7607341.1"
                     /translation="DSGSVRATLRFRQRGDGWLATELTLAGDVELECQRCLEPFRYEL
                     TESVNVVLAASDSLPATVPAGFEPFELDEARLKPAELIEDETIVAIPLVPKHARIEDC
                     GSLARKLAGQGERP"
     gene            388..579
                     /gene="rpmF"
                     /locus_tag="VFL84_01605"
     CDS             388..579
                     /gene="rpmF"
                     /locus_tag="VFL84_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET7607342.1"
                     /translation="MAVQKSRKTPSRRGMRRSHDALTGPTLSVDATTGVTHRRHHVTA
                     DGFYRGKKVYEIPQDAEQE"
     gene            645..1658
                     /gene="plsX"
                     /locus_tag="VFL84_01610"
     CDS             645..1658
                     /gene="plsX"
                     /locus_tag="VFL84_01610"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET7607343.1"
                     /translation="MSRKVTIALDAMGGDHGPEVVVPAALATLERDDSVSLTLVGLAD
                     VLERVKRQAGDRYGSRISFKAATEVVSMDEPPADALRRKKDSSLRVAIELVKSGEADA
                     CVSSGNTGALMATARYVLKTLPGIDRPAIISAVPGIAGPTYMLDLGANVGCTPEHLLQ
                     FALMGSVVAEDMLEVARPRVGLLNIGLEDMKGNETVREAGELIARSGLNYVGFVEGDG
                     IFHADVDVVVSDGFVGNVALKTMEGVARLMSAFLKEEFRRSLLRGMQGLVARPALEAL
                     GCRLDPRRYNGASLVGLNGIVLKSHGSADQFAFENAVKAAIVEARKGVPLQIGKLVEN
                     RPH"
     gene            1659..2612
                     /locus_tag="VFL84_01615"
     CDS             1659..2612
                     /locus_tag="VFL84_01615"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET7607344.1"
                     /translation="MYSRIAGTGKYLPQRVLTNADLEKMVETSDEWIKSRTGIELRHI
                     AAENEYTSDLAYKAGLAALESAGVTPGDIDLVVVGTTTPDLIFPNVACLVQEKLGIKS
                     CPAFSVEAACSGFLYGLVVADQFVRLGQSKRALVIGAETMSRIIDWTDRETCVLFGDG
                     AGAVVLEAAEGPGIIYSTLGADGRYRDLLYASSGVSLRQREGAALRMKGNEVFKVAVK
                     TLENLVDDVVEKNNLEKGQIDWLIPHQANIRIISATAKRLELPMERVVLTVREHGNTS
                     AASVPMALDTAIRDGRIKRGDLLLLEAFGGGFTWGASLIRY"
     gene            2679..>3644
                     /locus_tag="VFL84_01620"
     CDS             2679..>3644
                     /locus_tag="VFL84_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET7607345.1"
                     /translation="MPRLAHFALLSFLCVATAQAEEFWIGEGDEVFGEVRVIEARFED
                     TFVGLARTYNVGFEELKQANPGVDAWLPGAGTKIAIPTLYVLPRAAQKGIVVNVAELR
                     LYYFPADSKPPPEGVPSGSRRVITHPISIGRLDWSTPIGVTTITGKVANPSWYPPQSI
                     RDEHAARNDVLPRVVPPGPDNPLGKHAMRLALGSYLIHGTNKPSGVGMRVTHGCIRMF
                     PEDIEALYKTVPVGTSVNIVNQPVKLGWKADGTLYLEAHPALHESNFDGESVADDVAA
                     EPVQESPLTSLMRAYIAATEDRRVELDWDAAERVAQGGSGIPEAIS"
BASE COUNT          637 a         1149 c         1235 g          623 t
ORIGIN      
        1 gattccggca gtgtaagagc aacgctgcgc tttcgacagc gtggcgacgg ttggttggcg
       61 acggagctta cgctcgcggg cgacgtcgag ctcgagtgtc agcgctgcct cgagccgttt
      121 cgctacgagc tcacggagag cgtgaacgta gtgctcgccg ccagcgactc actgccggcg
      181 acggtgccgg cgggattcga gccgttcgag ctggacgagg cgcgattgaa gccggcggag
      241 ctgatcgagg acgagacgat cgtcgcgatt ccgttggtgc ccaagcacgc gcgcatcgag
      301 gactgtggta gcttggcgcg gaagctcgcc gggcagggtg agcggccttg agaaggccgc
      361 tcgaagtttt gttcgaggag atttcagatg gcagttcaaa agagccgcaa gacgccttcc
      421 cggcgtggca tgcgtcggtc gcacgatgca ttgaccggcc cgacgttgtc cgtggatgcc
      481 accaccggag tcacgcatcg gcgtcaccac gtcacggccg acgggttcta ccgcggcaag
      541 aaggtctacg agatcccgca ggacgccgag caagagtagt cgcgttggat tcgcgctgcc
      601 ggcggccgcg tagcgttagc gccgcgctcg aatcctaatc cgacgtgagt cgcaaggtca
      661 caatcgcctt ggacgccatg ggcggagacc atgggcccga agtcgtcgtc cctgcggctc
      721 tcgcgactct cgagcgcgac gactccgtga gcctgacgct cgtcggcttg gccgacgtgc
      781 tcgagcgcgt caagcgccag gccggcgatc gctacggctc gcgcatctcg ttcaaagcag
      841 cgaccgaagt cgtctcgatg gacgagccgc cggccgacgc gctgcgccgc aagaaggatt
      901 cgtcgttgcg cgtcgcgatc gagctcgtga agagcggcga ggcggacgcc tgcgtcagct
      961 ccggcaacac gggcgcgctg atggcgacgg cgcgctatgt attgaagacg ctgcccggca
     1021 tcgaccggcc ggcgatcatc tccgcagtgc ccggaatcgc aggccccacc tacatgctcg
     1081 atctcggcgc aaacgtcggc tgcacgccgg agcacttgtt gcagttcgcg ctgatggggt
     1141 ccgtcgtcgc cgaggacatg ctcgaggtcg cgcggccgcg cgtcggcctc ttgaacatcg
     1201 gcctcgagga catgaagggc aacgaaaccg tgcgcgaggc cggcgagctg atcgcgcgca
     1261 gcggcttgaa ctatgtcggt ttcgtcgagg gcgacgggat cttccatgcc gacgtcgacg
     1321 tcgtcgtcag cgacgggttc gtcggcaacg tcgcattgaa gacgatggag ggtgtcgctc
     1381 ggctcatgag cgccttcttg aaggaggagt ttcgccgtag tctgttgcgt ggtatgcagg
     1441 gtctcgtcgc gcggccggcg ctcgaggcgt tgggctgcag gttggacccg cgtcgctaca
     1501 acggagcgag cctcgtcggg ctcaacggca tcgtattgaa gagccacggc tctgcggacc
     1561 agttcgcgtt cgagaacgcc gtcaaggcgg cgatcgtcga ggcgcgtaag ggcgttccac
     1621 tccaaatcgg caagctcgtc gagaatcggc ctcattagat gtattcgcgt atcgccggca
     1681 ccggcaaata ccttccgcag cgtgtcctca ccaacgcgga tctcgagaag atggtcgaga
     1741 ccagcgacga gtggatcaag agccgcacgg gcatcgagct gcggcacatc gccgccgaga
     1801 acgaatacac gagcgatctc gcgtacaagg cgggtctcgc ggcgctcgag agcgccggcg
     1861 tcactcccgg cgacatcgat ctcgtggtcg tgggcacgac gactccggac ctgatctttc
     1921 cgaacgtcgc ctgtctcgtg caggagaagc tcggcatcaa gagctgcccc gcgttcagcg
     1981 tcgaagcggc ttgctcgggg tttctgtacg gcctcgtcgt tgcggatcag ttcgtgcgct
     2041 tggggcaatc caagcgcgct ctcgtgatcg gcgcggagac gatgtcgcgg atcatcgact
     2101 ggaccgatcg cgaaacctgc gtgctgttcg gcgacggcgc cggcgcggtc gtcctcgagg
     2161 ccgccgaggg ccccggaatc atctattcga ctttgggcgc ggacgggcgc tatcgcgact
     2221 tgctttacgc ctcgagcggc gtgtcgctca ggcagcgcga gggtgcggcg ctgcgcatga
     2281 aaggcaacga ggtcttcaaa gtcgccgtga agacgctcga gaacctcgtc gacgacgtcg
     2341 tcgagaagaa caatctcgag aagggccaga tcgactggct catcccgcac caagccaaca
     2401 tccgcatcat cagcgcgacc gcgaagcggc tcgagttgcc catggagcgg gtcgttctga
     2461 ccgtgcgcga gcacggcaat acctcggccg cgtcggtgcc gatggcgctc gacaccgcga
     2521 tccgggacgg ccgcatcaag cgcggcgacc tgctgctgct cgaggcgttc ggcggtggtt
     2581 tcacgtgggg cgcgagcctg atccgctatt gagcgccgga tcaaagactg ccgactggcg
     2641 ctcatattca tcgctttcgc gatacgtata atgcgcgcat gcctcgtcta gcacatttcg
     2701 cgttgctctc cttcctctgc gtcgcgacgg cgcaggcgga ggagttctgg atcggcgaag
     2761 gcgacgaggt cttcggcgag gttcgagtca tcgaagctcg gttcgaagac acgttcgtgg
     2821 gcctcgcgcg cacgtacaac gtcggcttcg aggagctcaa gcaagcgaat cccggcgtcg
     2881 acgcgtggct gcccggcgcg ggcacgaaga tcgcgattcc gacgctctac gtgctgccgc
     2941 gggcggcgca gaagggcatc gtcgtcaacg tcgcggagct gcggctgtac tacttcccgg
     3001 ccgattcgaa gccgccgccc gaaggggtgc cctcagggtc gcgccgcgtc atcacccatc
     3061 cgatcagcat cggccgcttg gactggtcga cgccgatcgg cgtcacgacg atcacgggta
     3121 aggtcgcaaa cccgagctgg tatccgccgc aatcgattcg ggacgagcat gcggcgcgca
     3181 acgatgtctt gccgcgcgtc gtgcctccgg gccccgataa cccgctcggc aagcacgcga
     3241 tgcgtctcgc gctcggcagc tacttgatcc acggaacgaa caagccgtcc ggcgtcggca
     3301 tgcgcgtcac ccacggctgc attcgcatgt tccccgagga catcgaagcg ctatacaaga
     3361 cggtgccggt cggcacctcc gtcaacatcg tcaatcaacc ggtcaagctc ggttggaagg
     3421 ccgacgggac gttgtacttg gaggcgcatc ccgcgctgca cgagagcaac ttcgacggcg
     3481 aaagcgtcgc ggacgacgtc gcggccgagc cggttcagga atcgccgctg acgagcctca
     3541 tgcgcgcgta tatcgcggcc accgaagatc ggcgcgtcga gctcgactgg gacgcggccg
     3601 agcgcgtcgc gcagggcggc agcggtatac ccgaagccat ctcg
//
LOCUS       DASSNN010000089         4256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_19513, whole genome shotgun sequence.
ACCESSION   DASSNN010000089 DASSNN010000000
VERSION     DASSNN010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19513"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1184
                     /locus_tag="VFL84_01625"
     CDS             75..1184
                     /locus_tag="VFL84_01625"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HET7607346.1"
                     /translation="MREEKFLDLTLALDSLTGASPSGAMPAGAVQTFTNPSGNAQYTI
                     APGEQALDPSFLDTRTALSANWEMPVGRLSLLSVGASLSDEYDYTHTGVNANLARDFN
                     NRNTTLSFGVALANDSIDPVGGSPVALAPMLGLGNSANKRGDESKDVTDFLLGITQVL
                     SRHSIVQLNYSLSQSDGYLTDPYKVLSVVDPVSGNPVAGPAGSGLFRYLYESRPDTRD
                     KQSVYALWKRDFDGDVLDLSYRYMTDDWGVDSHTVEARYRFAFGTGKYLQPHVRFYQQ
                     TAADFYRTVLFDGAPLPSYASADHRLGEFDGLTVGLKYGQATRGGGEWWARVEYYTQT
                     GDASPGSSVGALANFDLYPDLNALIAQFSYKFGRR"
     gene            1192..2091
                     /locus_tag="VFL84_01630"
     CDS             1192..2091
                     /locus_tag="VFL84_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET7607347.1"
                     /translation="MAPEPAAGASLSRRGSGWLGTFQSMASPCEVHVGEADLATADRV
                     VAIAAAEARRIEAKFSRYLRGNVIDAINTAEGRPVVVDDETARLLDYAARLHELSDGK
                     FDVTSGVLRRAWRFDGSDRVPTPEAVAALLPIVGWDKVRWRAPELRLPRGMEIDLGGI
                     GKEYAVDSAAGLVRSVSSRCLLNFGGDLLALGPQPGGSPWRVGVESVSDVGAVAKQID
                     LTVGALATSGDSRRFLQRDGKRYGHVLDPRTGWPVAGGPRSVTVAASTCTEAGMLATL
                     ALLQGADAEAFLEAQGVQFWALR"
     gene            2159..2545
                     /locus_tag="VFL84_01635"
     CDS             2159..2545
                     /locus_tag="VFL84_01635"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7607348.1"
                     /translation="MKVVWSVLAVALLAAGSAEAHHSFAAQYDPQKPVTLKGKVTKIE
                     WTNPHARFYIDVPGADGKVVNWNLELASPNYLKRAGWSSGSLKQGDEVTVEGSMARSG
                     ANMANARLVTFPDGTRVFARSAQDGG"
     gene            2549..3346
                     /locus_tag="VFL84_01640"
     CDS             2549..3346
                     /locus_tag="VFL84_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607349.1"
                     /translation="MRIQWLIAAFAAGALAQIASAQQAPRDATSPAGSGGSSNPALVG
                     AVPEGPPPRLPDGSPDLSGVWVGAGPIGDISTGLMPGEEIVMLPAAQALMKSRMAADD
                     PEARCLPTGVPRIAPYPWRLVQSPAYGKATHLFFLFEGNIHSFRQIFMDGRPHPEDEE
                     PSWYGHSVGHWEGDTLVIETVGFNDLFWFDFVGHPHTTQLKTVERYTRTKFGELEVVT
                     TVTDPGAYAKPFTVKFRHTLRPGWELMEYICNENNQDVEHIRGPAGL"
     gene            3343..3726
                     /locus_tag="VFL84_01645"
     CDS             3343..3726
                     /locus_tag="VFL84_01645"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET7607350.1"
                     /translation="MRAFARDVGVFTIVGTTAMVLNLTVVSVAVPIGMTPLSANVVGF
                     LFSFAWSFFGHARWTFPAQGRDVGVAMWRFAIVSLISFALTEAAYAGALEWTSVDYRL
                     SLFLVIVTLSLGKLLASRHWAFARA"
     gene            complement(3828..>4256)
                     /locus_tag="VFL84_01650"
     CDS             complement(3828..>4256)
                     /locus_tag="VFL84_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607351.1"
                     /translation="VLGAGIASNHWAALLAFAGTYTLMYQAVTLAEEHYLRGKFGAAF
                     DEYCRRVPRWWLRMAGIRETFERTRFNWNRVLIKAYSEPLGWTLPIVLIGIWNIHRTT
                     GLAARPVAVDVLWALLGVAALIWITLGTLKKTRSLAPSET"
BASE COUNT          681 a         1424 c         1448 g          703 t
ORIGIN      
        1 cgtcgctcta gatttacagc gaatccgacg gccgcgttca ggatctgagc ctgaatcttc
       61 gcgcgcgcaa ggaaatgcgc gaggagaagt tcctggacct gacgctcgcg ctcgactcgt
      121 tgacgggcgc gagcccgagc ggcgcgatgc cggccggcgc ggtgcaaacg ttcacgaacc
      181 cgtccggcaa cgcgcagtac acgatcgcgc cgggcgagca agcgctcgac ccgtcgttcc
      241 tcgacacgcg cacggcgctc tcggcgaatt gggagatgcc ggtcgggcgc ctttcgttgc
      301 tctcggtcgg cgcgagcttg tccgacgagt acgattacac gcataccggc gtcaacgcga
      361 acctcgcacg cgacttcaac aaccgcaaca cgacgctgtc gttcggcgtt gcgctcgcga
      421 acgactcgat cgatcccgtc ggcggcagcc ccgtcgcgct cgcgccgatg ctcggcctcg
      481 gcaactccgc caataagcgc ggcgatgaat cgaaggacgt cacggatttc ttgctcggga
      541 tcacgcaggt cctgagccgc cactcgatcg tccagctcaa ctactctctg agccagtcgg
      601 acggctacct caccgatcct tacaaggtcc tgtccgtcgt cgacccggtc agcggcaatc
      661 cggtcgccgg acccgccggc agcggcctct ttcggtacct ctacgagagc cggcccgaca
      721 cgcgcgacaa gcagagcgtg tacgcgctgt ggaagcgcga cttcgacggc gacgtgctcg
      781 atctgtcgta ccgctacatg accgacgatt ggggcgtcga ttcgcacacg gtcgaggcgc
      841 gttaccgttt cgcgttcggc accggcaagt acctgcaacc gcatgtccgc ttctatcagc
      901 agaccgcggc ggacttctat cgaacggtgc tgttcgacgg agcgccgttg ccgagctacg
      961 cgagcgccga ccatcggctc ggcgagttcg acgggctcac cgtcggcttg aaatacggcc
     1021 aggcgacgcg cggcggcggc gaatggtggg cgcgcgtcga gtactacacg cagaccggcg
     1081 acgcgtcgcc gggaagctcg gtcggcgcgc tcgcgaattt cgacctgtac cccgatctga
     1141 acgcgctgat tgctcagttc agctacaagt tcgggcggcg ctgaacgtcc catggcgccg
     1201 gagccggcag cgggcgcgtc tctctcgcgg cgcggctcgg gctggctcgg cacttttcaa
     1261 tcgatggcga gcccgtgcga ggtccacgtc ggcgaggcgg acctcgcgac cgcggatcgc
     1321 gtcgtcgcga tcgcggccgc cgaagcacgg cggatcgagg cgaagttcag ccgttatctg
     1381 cgcggcaacg tcatcgacgc gatcaacacg gccgaaggcc gccccgtcgt cgtcgacgac
     1441 gagaccgcgc gcttgctcga ttacgcggcg cggctccacg agctcagcga cggcaagttc
     1501 gacgtgacgt cgggcgtatt gcggcgcgcc tggcgcttcg acggcagcga ccgcgtgccg
     1561 acgcccgaag ccgttgccgc gctgttgccg atcgtcggct gggacaaggt ccgctggcgc
     1621 gcgcccgagc tcaggctgcc gcgcggcatg gagatcgatc tcggcggcat cggcaaggag
     1681 tacgccgtcg acagcgcggc gggcctcgtg cgctccgtat cttcgcggtg cctcctgaac
     1741 ttcggcggcg acttgctggc gctcgggccg cagcccggcg gctcgccgtg gcgcgtcggc
     1801 gtcgaatcgg tcagcgacgt cggggccgtc gcgaaacaga tcgatctcac ggtgggcgct
     1861 ctcgcgacga gcggcgattc gcgccgcttc ttgcagaggg acggcaagcg ctacggccac
     1921 gtcctcgacc cgcggacggg ctggcccgtg gcgggcgggc cgcgctcggt cacggtcgcc
     1981 gcgtcgacct gcacggaggc gggcatgctc gcgacgctcg cgctgctgca gggggccgac
     2041 gccgaggcgt tcctcgaggc ccagggcgtg caattctggg cgctgcgtta agccgaagac
     2101 tcgacacctc gccggagtcg gggctagagt tctacgagga actcgggagg ggagaccgat
     2161 gaaggtcgtt tggagtgttc tcgcagtcgc gctgctcgcg gccgggtcgg ccgaagccca
     2221 tcattcgttc gcggcacaat acgacccgca gaagccggtg acgctgaaag gcaaggtcac
     2281 gaagatcgag tggacgaacc cgcacgcgcg gttctacatc gacgtgcccg gcgccgacgg
     2341 caaggtcgtg aactggaacc tcgagctcgc gagcccgaac tatctgaagc gcgcgggctg
     2401 gtcgagcgga tcgctgaagc aaggcgacga ggtgaccgtc gaaggttcca tggcgcgcag
     2461 cggcgcgaac atggcgaacg cgcggctcgt gaccttcccc gacggaacgc gcgtattcgc
     2521 gcgctcggcg caggacggcg gctagcacat gcgcatccaa tggttgatcg cggcgttcgc
     2581 cgcgggcgca ctcgcgcaaa tcgcgtcggc tcaacaagcg ccgcgcgacg cgacctcgcc
     2641 ggccggcagc ggcgggtcga gcaatccggc gctggtcgga gcggtgcccg aaggcccgcc
     2701 gccgcggttg ccggatggca gcccggattt gtcgggcgtc tgggtcggcg cggggccgat
     2761 cggcgacatc agcacgggcc tcatgccggg cgaggagatc gtgatgctgc ccgcggccca
     2821 ggcgctcatg aaatcgcgca tggccgccga cgatcccgag gcgcgctgct tgccgacggg
     2881 ggtgccgcgc atcgcgccgt atccgtggcg gctcgtgcag tcgccggcgt acggcaaggc
     2941 gacccatcta tttttcttgt tcgagggcaa catccatagc ttccggcaaa tcttcatgga
     3001 cggccggccg catcccgagg acgaggagcc ttcttggtac ggccactcgg tcgggcactg
     3061 ggagggcgac acgctcgtga tcgagaccgt cgggttcaac gatctcttct ggttcgactt
     3121 cgtgggtcat ccgcacacga cgcagctcaa gaccgtcgaa cgctatacgc gcacgaagtt
     3181 cggcgagctc gaggtcgtga cgacggtcac cgatcccggc gcgtatgcga agccgttcac
     3241 cgtgaaattc cggcacacgc tgcggcccgg gtgggagctc atggagtaca tttgcaacga
     3301 gaacaatcag gacgtcgagc acatccgcgg ccccgctggt ctttgagagc tttcgcgcgc
     3361 gacgtcggcg ttttcaccat cgtcgggacc acggcgatgg tcttgaacct caccgtcgtt
     3421 tcggtcgccg tcccgatcgg catgacgccg ctctcggcga acgtcgtcgg ctttctattt
     3481 tcgttcgcat ggagcttctt cggccacgcg cgctggacgt ttccggcgca agggcgcgac
     3541 gtcggtgtcg caatgtggcg tttcgcgatc gtctcgctga tcagcttcgc gctcaccgag
     3601 gcggcgtacg cgggggcgct cgaatggacg agcgtcgact atcggctgtc gctgttcctc
     3661 gtcatcgtca cgctgtcgct cggcaagctg ctcgccagca ggcactgggc gttcgctcgg
     3721 gcctgacgcg gcttccagaa gcggagcagc gagcgccgcc ctcggtccgc gccttcgcgc
     3781 tcgtcgtcgc cgcatcgtcg gcggccggag cgcgcacggc ccgcaattca ggtctccgac
     3841 ggcgcgagcg agcgtgtctt cttcagagtg cctagcgtga tccagatcag agcggcgacg
     3901 ccgagcaaag cccacaagac gtcgacggcg acgggccgcg cggcgaggcc cgtggttcgg
     3961 tggatgttcc agatcccgat cagcacgatc ggcagcgtcc aaccgagcgg ttcgctatat
     4021 gccttgatga gcacgcggtt ccaattgaag cgcgttctct cgaacgtttc ccgaatcccc
     4081 gccatccgca gccaccagcg cggtacgcgc cggcaatact cgtcgaacgc cgcgccgaac
     4141 ttgccgcgca gatagtgctc ctcggcaaga gtgaccgctt gatacatcag cgtgtacgtg
     4201 cccgcgaatg cgagcagtgc ggcccagtgg ttcgaagcga tgccggcgcc gagcac
//
LOCUS       DASSNN010000090         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_766023, whole genome shotgun sequence.
ACCESSION   DASSNN010000090 DASSNN010000000
VERSION     DASSNN010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766023"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /gene="glyA"
                     /locus_tag="VFL84_01655"
     CDS             <1..677
                     /gene="glyA"
                     /locus_tag="VFL84_01655"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239205.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET7607352.1"
                     /translation="GAKLMADIAHIAGLVAVGLHPTSVGKADFTTTTTHKTLRGPRGG
                     MILCDASHAKEIDRAVFPGLQGGPHNHTTAAIAVALKEASTPDFRSYGQQIVKNAKAL
                     AAALQARGFRLISGGTDNHLILIDVTPRGITGKPAARALHEAGIECNYNTIPFDPRKP
                     FDPSGLRIGTPSITSRGMKEKDMEPIAAWIDKVLSVADDASKLHKIRAEIAEFCKAFP
                     APGIRV"
     gene            complement(698..>2253)
                     /locus_tag="VFL84_01660"
     CDS             complement(698..>2253)
                     /locus_tag="VFL84_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7607353.1"
                     /translation="TVYTPLFSGVFWDQQDVVLADVDRIEVVSGPGAALWGANAVNGV
                     INVITRSAAETQGGLLSVGGGNFESQATVRYGGRLGDSGHYRAYAKTARFDATQRANG
                     SSALDAWSRDQAGFRADWSLDDAELTLQADVYDGRSQDRGEAFGFTFGRIEVSGANVL
                     GRWTRTLPNGSQLRVQSYFARAERDDSLFFRPRSNTFDVEAQHGLEIAAHDLLWGGGY
                     RRTSDDIGTGFITTFIPASRDLEWESAFVQDRIQLSPKLETTIGLKLERNDYTGTESL
                     PSVRLAWQPGHDRLVWTALSRAVRAPARFDRDVFFPGTPPFLVIGGPNFESEVANVAE
                     AGYRAQPRAAFNYSVTLFRHDWDKLRSGTAIPVQLENRIDGHAYGVEAWANWRVTERW
                     DIGGGLNTLNKDLVLEPGSTDPVGVDNPTLANDPEFQWLLRSSLQLPRGFNLELGVRH
                     VDSLPNPDVPRYTAVDGQLAWQPNRKLGLVLSVRNLFDAAHPEFEAEPNRSELPRTAY
                     FAVDWRFGG"
BASE COUNT          379 a          760 c          762 g          352 t
ORIGIN      
        1 tcggcgcgaa gctgatggcc gacatcgccc atatcgcggg gctcgtggcg gtcggcctgc
       61 acccgacctc ggtcggcaag gccgacttta cgaccaccac gacgcacaag acgctgcgcg
      121 gcccgcgcgg cggcatgatc ctgtgcgacg cgagccacgc gaaggagatc gatcgcgccg
      181 tgttcccggg cctgcaaggc ggtccgcaca accacacgac ggccgcgatc gccgtcgcgc
      241 tcaaggaagc atcgactccg gactttcgca gctacggcca gcaaatcgtc aagaacgcga
      301 aggcgttggc cgcggcgctg caagcgcgcg gcttccgctt gatctcgggc ggcaccgaca
      361 accacctgat cttgatcgac gtgacgccgc gcggcatcac gggcaagccg gcggcgcgcg
      421 cgctgcacga ggccggcatc gagtgcaact acaacacgat cccgttcgac ccgcgcaagc
      481 cttttgaccc ttcgggcctg cgcatcggca cgccgtcgat cacgtcgcgc ggcatgaaag
      541 agaaggacat ggagccgatc gcggcgtgga tcgacaaggt cctctcagtc gccgacgacg
      601 cgagcaagct gcacaagatc cgcgccgaga tcgcggaatt ctgcaaggcg tttccggcgc
      661 cggggatccg cgtctagcga cgcctcagcg atcgagctca gccgccaaag cgccagtcga
      721 ccgcgaagta cgcggtgcgc ggcagctcgc tgcgattcgg ctccgcttcg aactccgggt
      781 gtgccgcgtc gaacaggttg cgcacgctga gcacgagacc gagcttccga ttcggctgcc
      841 atgccagctg gccgtcgacg gccgtgtagc gcggcacgtc cgggttcggc agggaatcga
      901 cgtggcgtac gccgagctcg aggttaaagc cgcgcggcaa ttgcagtgac gagcgcagca
      961 accattgaaa ctccgggtcg ttcgcgaggg tcgggttgtc gacgccgaca gggtccgtgc
     1021 tgcccggctc cagcacgagg tccttattaa gcgtgttcag gccgccgccg atgtcccagc
     1081 gctccgtcac gcgccaattc gcccacgctt cgacgccata ggcgtggccg tcgatccggt
     1141 tctcgagctg cacggggatc gccgtgccgc tgcggagctt gtcccagtcg tgccggaaca
     1201 gcgtcaccga ataattgaac gccgcgcgcg gctgcgcgcg gtagccggcc tcggcgacgt
     1261 tggcgacctc ggactcgaag ttcgggccgc cgatgacgag gaacggcggc gtgccgggga
     1321 aaaagacgtc gcggtcgaat cgcgccggcg cgcgcacggc tcgcgatagc gccgtccaca
     1381 cgagccggtc gtgacccggc tgccaggcga gccgcacgct cggcagcgat tcggtgccgg
     1441 tgtagtcgtt gcgctcgagc ttgaggccga tcgtggtctc gagcttgggg ctcaattgga
     1501 tgcggtcttg aacgaatgcg ctctcccact cgagatcgcg gctcgcgggg atgaaggtgg
     1561 tgatgaaacc ggtgccgatg tcgtcgctcg tgcgtcgata gccgccgccc cagagcaaat
     1621 cgtgagcggc gatctcgagg ccgtgttggg cctcgacgtc gaacgtgttg ctgcgcggcc
     1681 gaaagaacag cgaatcgtcc cgctcggcac gagcgaaata gctttggacg cgcagctgcg
     1741 acccgttcgg caaggtgcgc gtccaacggc cgagcacatt cgcgcccgag acttcgatgc
     1801 ggccgaacgt gaagccgaag gcctcacccc gatcttggga gcggccgtcg tagacgtcgg
     1861 cttgcagcgt cagctcggcg tcgtcgaggc tccagtctgc gcggaagccg gcttgatcgc
     1921 gcgaccacgc gtcgagtgcg ctgctgccgt tcgcgcgttg cgtcgcgtcg aaccgggccg
     1981 tcttcgcgta cgcccgatag tgccctgagt cgccgagccg gccgccgtag cggaccgtcg
     2041 cttggctctc gaaattgccg ccgcccaccg agagcaagcc gccttgcgtc tcggccgccg
     2101 aacgagtaat cacgttgatg acgccgttca cggcgttcgc gccccacagc gccgctccgg
     2161 ggccgctgac gacctcgatg cggtcgacgt cggcgagcac gacgtcctgt tggtcccaaa
     2221 acactcccga gaacaacggc gtgtagacgg tgc
//
LOCUS       DASSNN010000091         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_524003, whole genome shotgun sequence.
ACCESSION   DASSNN010000091 DASSNN010000000
VERSION     DASSNN010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524003"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFL84_01665"
     CDS             <1..378
                     /locus_tag="VFL84_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET7607354.1"
                     /translation="SLLQELVDRGFYSQEEAQRSTNRNYVTRALGVEANVDVEVQEIE
                     VQKGDYFLMCSDGLPDMVEDEDIHLTISTFANDVRTVGEQLIKLTNDNGGRDNVSVIL
                     VRVADAFPAQSGFISKFRGLFGS"
     gene            426..1214
                     /locus_tag="VFL84_01670"
     CDS             426..1214
                     /locus_tag="VFL84_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7607355.1"
                     /translation="MARLILSLDGQTLAEYNMTKERYTVGRLPDNDIRIDNAAVSGHH
                     SLLINILNDSFLEDLNSTNGTYVNGKLIKKHALQHGDVITVGHHQLRYVDDQAQQQPE
                     DEFEKTMVISQPSAPLENKVAQAARAADAAANAAAVTEAPRPPPPEPAAKPRRPFSPT
                     TTQEVDPEAAPALPLAKLQVLSGTFAGRELSLTKTLTTLGRPGVQVAAITRRAEGYYI
                     VHVESGKTDGFPMVNGMAIGAQARKLQDNDVIQLAGVKMGFFLN"
     gene            1500..>2461
                     /locus_tag="VFL84_01675"
     CDS             1500..>2461
                     /locus_tag="VFL84_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7607356.1"
                     /translation="MLSFQPGSAAERAGFQAGDLIRRFGTVDVATPVDVIDAVAAAKV
                     GERVAVVVERNGQRVELQVEFMAADVRGPPPAVASVRAPAVDPCATPSDEESSRVVWR
                     PPYERLPLPADAGELLALVDDTDTAVVVEANVLSIVRRDAREALVVTGTFQCPMSKIP
                     GHDVWALQLRAARWDTAFFSYAFRSASGPAFDGFRSFRGADAPPLPVEKETLTGRLIE
                     TSITSRQLSEQRSITVYLPPGASEPNMPALFMTDGQAASEYARVLEPLIESGAVPAIA
                     IVGVHSGGERRAREYLSGVDEQAFAKHLAFFVDEVLPWASAEYG"
BASE COUNT          437 a          810 c          814 g          400 t
ORIGIN      
        1 tcgctgctgc aggagctcgt cgatcgcggc ttctactcgc aggaggaagc gcagcgctcg
       61 accaaccgca actacgtgac gcgcgcgcta ggcgtcgagg cgaacgtcga cgtcgaagtg
      121 caggagatcg aggttcagaa aggcgactat ttcctgatgt gctcggacgg gctgccggac
      181 atggtcgagg acgaggacat ccatttgacg atcagcacgt tcgcgaacga cgttcgcacg
      241 gtcggagagc aactgatcaa gctgacgaac gacaacggcg ggcgcgacaa cgtctcggtc
      301 atcctcgtgc gcgtcgccga tgctttcccg gctcaatccg gtttcatcag caagttccgc
      361 ggtctgttcg ggtcgtagac gtagaattgg taatcgaata actaaaggca attgggacga
      421 ctaccatggc acgcttgatt ctcagcttgg acggccagac cttggcggaa tacaacatga
      481 ccaaggaacg ctacacggtc ggccgcttgc cggataacga cattcgcatc gacaacgccg
      541 cggtcagtgg tcatcactcg ttgctcatca acatcctgaa cgactccttc ctcgaagacc
      601 tgaacagcac gaacggaacc tacgtcaacg gcaagctgat caagaagcac gctctgcagc
      661 acggcgacgt catcacggtc ggccatcatc agctccgcta cgtcgacgat caggcgcagc
      721 agcagcccga ggacgagttc gagaagacga tggtgatcag ccagccgagc gcgccgctcg
      781 agaacaaggt cgcgcaggcg gcgcgcgccg ccgacgccgc tgcgaacgcc gcggcagtga
      841 cggaagcgcc gcgtccgcct ccgcccgagc ccgccgcgaa gccgcgccgg ccgttctcgc
      901 cgacgacaac acaggaagtc gaccccgagg cggcaccggc tctgccgctc gcgaagctgc
      961 aagtgctgtc gggcacgttt gccggccgcg agctgtcgct gaccaagacg ttgacgactc
     1021 tcggccgccc cggtgtgcaa gtggccgcga tcacgcgccg cgccgagggc tactacatcg
     1081 tgcacgtcga gagcggcaag accgacggct tcccgatggt caacggcatg gcgatcggcg
     1141 cgcaagcgcg caagctccag gacaacgacg tcatccagct cgccggcgtg aagatggggt
     1201 tcttcctgaa ctagcgtccc ggtcgcgact cggcgttagg accccgccgc gttagactct
     1261 cgttgcgccc gcgttcgagc gtggggcacg aggagaagcc gatgcggtgc ggaatctggc
     1321 tgtctgcctt actgtgcgca acgcacattt gtgcctcgtc ggcgtcggcg caagcttcga
     1381 ggccggcggc accatcgcag ggcgccgaac gcctcacgct tggagtcgcg cttgcgattc
     1441 ccggagccgg cgtaacgcta tgcggcacgc aacctgcgag cgagccgcag gcggcgtgcg
     1501 tgctctcgtt ccagcccggc agcgccgcag agcgcgccgg gtttcaagcc ggcgacttga
     1561 ttcggcgttt cgggaccgtg gacgtcgcga cgccggtcga tgtcatcgac gccgtagcag
     1621 cggcgaaagt cggcgaacgt gttgccgtgg tcgtcgagcg caatggccag cgcgtggagc
     1681 tccaagtcga gttcatggcc gcagacgtgc gcgggccgcc tcccgcggtc gcgtccgttc
     1741 gcgcgcccgc ggttgatccg tgtgcgactc cgagcgatga agaatcgagc cgcgtcgtct
     1801 ggcggccgcc ttacgagcgg ctaccgcttc ctgccgacgc aggcgagctt ctcgcgctcg
     1861 tcgacgacac cgacactgcc gtcgtagtcg aggccaacgt gctgtcgatc gtgcggcgcg
     1921 atgcgcgcga ggcgctcgtc gtgacgggca cgtttcagtg tccgatgagc aaaatccccg
     1981 ggcacgacgt ctgggcactg cagctccgcg cggcgcgctg ggacacggcg ttcttcagct
     2041 acgcgttccg ctcggcctcg gggccggcgt tcgacggctt ccgctcgttc cgcggcgccg
     2101 acgccccgcc tctaccggtc gagaaggaaa cgctcacggg ccgcctcatc gaaacgagca
     2161 tcacgagccg gcagttgagc gaacagcgct cgatcacggt gtatttgcct cccggcgctt
     2221 cggagccgaa catgccggcg ctgttcatga ccgacgggca agcggcatcg gagtacgcgc
     2281 gcgtgctcga gccgctgatc gagtccggcg cagtgccggc catcgcgatc gtcggtgtgc
     2341 actcgggtgg cgagcgccgc gcgcgcgagt acctttccgg cgtggacgaa caggcattcg
     2401 cgaagcacct cgcattcttc gtcgacgagg tgctgccgtg ggcgagcgcc gagtacggca
     2461 t
//
LOCUS       DASSNN010000092         5099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_524345, whole genome shotgun sequence.
ACCESSION   DASSNN010000092 DASSNN010000000
VERSION     DASSNN010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524345"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFL84_01680"
     CDS             <1..191
                     /locus_tag="VFL84_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607357.1"
                     /translation="MTITQQPGYEIYEYACHEGNMAMRNILSAERAYERAAAEAAAKG
                     LPPPERVFERVNGPDRAR"
     gene            complement(237..881)
                     /gene="nth"
                     /locus_tag="VFL84_01685"
     CDS             complement(237..881)
                     /gene="nth"
                     /locus_tag="VFL84_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET7607358.1"
                     /translation="MNAAKRRKIYERFARANPEPKTELDYTTPFELLVAVVLSAQATD
                     VSVNKATAKLFPVANTPAAILELGEEGLKSYIKTIGLFNSKAKNLIKTCATLVAEHGG
                     EVPRDRETLESLPGVGRKTANVILNTAYGESTIAVDTHIFRVANRIGLAVGATVRAVE
                     DALVRVTPDEFKRHAHHWLILHGRYVCKARKPECPRCILVDLCEYPHKTPPAEP"
     gene            complement(887..1516)
                     /gene="rsxE"
                     /locus_tag="VFL84_01690"
     CDS             complement(887..1516)
                     /gene="rsxE"
                     /locus_tag="VFL84_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HET7607359.1"
                     /translation="MTPTTSSWHEATAPLWRDNMALAQLLGLCPLLAVTTTVVNGLAL
                     GLASAAVLVISSTAMAALKGVLTPAARLPMALLILAALVTVVDLLTEAVFYDLHGLLG
                     IFVPLIVVNSGLLAHARNVASRSSVAFTFLSALATGVGFLLAFIALGAFREIVGNGTL
                     LSGIELLAGERYSGLTLGLPVHGVLVAILPPGAFFGLGLLLALRNRLRR"
     gene            complement(1513..2169)
                     /locus_tag="VFL84_01695"
     CDS             complement(1513..2169)
                     /locus_tag="VFL84_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HET7607360.1"
                     /translation="MASAEGNWLLTGVAAAALAAAAFYVVHAAYDWSDERIAANERAR
                     IVARLNSVLEPALRGRDLTTTRLEVTDPEHLGTDDPVDVFVLTDRGAPMATVFATVAP
                     HGYNASIDLLIGVSPIGTVTGVRAVRHRETQGLGDAIDVAKSDWMLQFDGKALTAPPA
                     ERWAIRQDDGEFDAISGATVTSRAVVTAVKNTLLYFEAHRDELYRQAAEAAAAASNEP
                     "
     gene            complement(2156..3190)
                     /locus_tag="VFL84_01700"
     CDS             complement(2156..3190)
                     /locus_tag="VFL84_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019571414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HET7607361.1"
                     /translation="MHFDQSTAPHARPLVSVPVIMQRVLFALVPAMLCHTWYFGPGLL
                     VNFALTACAALVTEGAVLRLRGRPTRHALRDCSALVTAALLSFALPPFVPFWIPLIGG
                     AIAITLAKQLYGGLGKNLFNPAMVGYVFLILSFPVEMTQWLPPRMGDLDYQPLSVGEH
                     VNYVFTGRLPAELDVDAVTRATPLDLVKEGLRAGRPFAEIRGGSLFGDFGGRGWEWVA
                     NFIALGGLYLLYTGTIRWHIPTMFLLGLLTPATVLYLLDPSGYAAPGFHLFSGATMLG
                     AFFIATEPVSAAGTDRGRLIYGAGIGLLVYALRTWGAYPDGVAFSVLLMNGTVPLINR
                     YTRPRIYGQR"
     gene            complement(3191..4552)
                     /gene="rsxC"
                     /locus_tag="VFL84_01705"
     CDS             complement(3191..4552)
                     /gene="rsxC"
                     /locus_tag="VFL84_01705"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HET7607362.1"
                     /translation="MVDYVFERHLSGGILLPANKTSSTSSPIHRSFVPTKLVLALNQH
                     RGNAAEPVVAVGDRVLKGQTVAVATAAPSAAVHAGTSGFVLAIEERPALGGHGVQLSP
                     CIVIESDGRDERVPAAAAPEWPSSLAEQRDAIRAGGIVGLGGAVYPTADKLATPVPVQ
                     TLIVNGAECEPYISCDDMLMRENAAEIVAGTLILADVLAAPRAIIAIERDKPQAMEAI
                     EAAAQAADDARLKLAEVPTIYPAGGEKQLVELLTGEEVPSGRYPGEFGYVCQNVGTAY
                     AVQRLRALGEPLVTRVVTVTGGGVKRPQNVEAPIGTPIAELIALCGGYDEGVARLIAG
                     GSMMGYALPNDETPITKATNCIIAALADEVRTDMHEWPCIRCGDCAMVCPSRLLPQDL
                     LVAATTSDFAALHTLGLQDCIECGCCDVICPSQIMLTERFRIAKRSVAAGQSIPVTSP
                     GQD"
     gene            complement(4559..>5099)
                     /locus_tag="VFL84_01710"
     CDS             complement(4559..>5099)
                     /locus_tag="VFL84_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018866879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HET7607363.1"
                     /translation="NNFVLVQMLGVCPFIAGSQRVESALGMSLSLGLVLTIAAGATQL
                     VDRYLLRPYELSYLRLFALLLVIGVVVGVIDLAIRRFSPILQRVLGVYVPLVATNCAV
                     LGVALINTAASRSFVAALFYGTGAAVGFGLALTLFAALRERVVLADVPIAFRGAPITF
                     ITAGILSLAFMAFAGFARL"
BASE COUNT          857 a         1717 c         1783 g          742 t
ORIGIN      
        1 gcatgacgat cacgcagcag cccggctacg agatctacga gtacgcgtgc cacgagggca
       61 acatggcgat gcgcaacatc ttgtccgccg agcgcgccta cgagcgcgcg gcggccgagg
      121 cggccgccaa ggggctgccg ccgcccgagc gcgtgttcga gcgcgtcaac gggcccgacc
      181 gcgccaggta gatcaatagg ttacagtgct tttccaaagt atcgcttgca gtcgcgtcaa
      241 ggctccgcgg gcggcgtctt gtgcgggtat tcgcagaggt cgacgaggat gcagcgcggg
      301 cattcgggct tgcgcgcctt gcagacatag cggccgtgca agatcagcca gtggtgggcg
      361 tggcgcttga actcgtccgg cgtcacgcgc acgagcgcgt cctcgacggc gcgcacggtg
      421 gcgcccacgg ccaagccgat tcggttcgcg acgcggaaga tgtgcgtgtc gacggcgatc
      481 gtcgactcac cataggccgt gttcaagatc acgttcgccg tcttgcggcc gacgccgggc
      541 aacgactcga gcgtttcacg gtcgcgcggg acctcgccgc cgtgctcggc cacgagcgtc
      601 gcgcaggtct tgatcaggtt cttcgccttg ctgttgaaga gcccgatcgt cttgatgtag
      661 ctcttgagcc cttcctcgcc gagctcgagg atcgcggccg gcgtgttcgc gacgggaaat
      721 agcttcgcgg tcgctttgtt gacgctgacg tcggttgcct gcgccgagag cacgacggcg
      781 acgaggagct cgaacggcgt cgtgtaatcg agctccgtct tcggctccgg attggcccgc
      841 gcgaaacgtt cgtagatttt gcgccgcttg gccgcgttca ccgcggctac ctgcggagac
      901 ggttgcgcag ggcgagcaac aggcccaagc cgaagaacgc gccgggcggc aagatggcga
      961 cgagcacgcc gtgcacgggc agcccgagcg tcaggccgct gtagcgctcg ccggcgagca
     1021 gctcgatgcc ggacaggagc gtgccgttgc cgacgatctc gcgaaacgcg ccgagcgcga
     1081 tgaacgcgag caggaagccg acccccgtcg cgagcgcgga caggaacgtg aatgcgacgc
     1141 tgcttcggct cgcgacgttc cgggcgtgcg cgagcaggcc cgagttcacg acgatcagcg
     1201 gcacgaagat tccgagcagg ccgtgcaagt cgtagaacac cgcttcggtg agcaaatcga
     1261 cgacggtgac gagcgcggcc aggatcagca gcgccatcgg cagccgcgcg gccggcgtca
     1321 gcacgccctt cagcgcggcc atcgccgtgc tcgagatgac gaggacggcg gcgctcgcca
     1381 atccgagcgc caagccgttg actacggtcg tcgtcacggc gagcagcggg cagagcccga
     1441 gcagctgcgc gagcgccatg ttgtcgcgcc acagcggtgc cgtcgcttcg tgccagctgc
     1501 tcgtcgtcgg ggtcatggct cgttgctcgc tgcggccgcg gcttcggccg cttgcctgta
     1561 cagctcgtcg cgatgcgctt cgaagtatag caacgtgttc ttgacggccg tgacgacggc
     1621 gcgcgacgtg acggtggcgc ccgagatcgc gtcgaactcg ccgtcgtctt gccggatggc
     1681 ccagcgctcg gccggcggcg ccgtcagcgc tttgccgtcg aactgcagca tccagtcgct
     1741 tttcgcgacg tcgatcgcgt cgcccagccc ttgcgtctcg cggtgccgaa cggcgcgcac
     1801 gccggttacc gtgccgatcg gcgagacgcc gatcaacaag tcgatcgagg cgttgtagcc
     1861 gtgcggcgcg accgttgcga acaccgtggc catcggcgcg ccgcgatccg tcagcacgaa
     1921 cacgtccacg ggatcgtcgg tgcccaagtg ctcgggatcc gtgacctcga gccgcgtcgt
     1981 cgtgagatcg cggccgcgca gcgcgggctc caagacgctg ttcaagcggg ccacgatgcg
     2041 cgcgcgctcg ttcgccgcga tgcgctcgtc gctccagtcg tacgccgcat gcacgacata
     2101 gaacgccgcg gccgcgagcg cggccgccgc cacgcccgtg aggagccagt tgccttcagc
     2161 gctggccata gattcgcggc cgcgtgtagc ggttgatcag cggcaccgtg ccgttcatga
     2221 gcagcaccga gaacgcgacg ccgtccggat acgcgcccca cgtgcgcagc gcgtagacga
     2281 gcaagccgat gccggcgccg tagatgagcc ggccgcgatc cgtgcccgcc gccgatacgg
     2341 gctccgtcgc gatgaagaat gcgccgagca tcgtggcgcc gctgaacagg tggaagcccg
     2401 gcgccgcata gccgctcggg tcgagcagat agagcaccgt cgcaggcgtc agcagcccca
     2461 acagaaacat cgtcggaatg tgccagcgaa tcgtgcccgt gtagagcagg tagaggccgc
     2521 cgagcgcgat gaagttggcg acccattccc agccgcggcc gccgaagtcg ccgaacaacg
     2581 agccgccgcg aatctcggcg aacggccggc ccgcgcggag gccctccttg accaagtcga
     2641 gcggcgtcgc gcgcgtgacc gcgtcgacgt cgagctccgc gggcaagcgg cccgtgaaca
     2701 cgtagttgac gtgctcgccg acggatagcg gctggtaatc gagatcgccc atgcgcggcg
     2761 gcagccactg cgtcatctcg acgggaaacg acaagatcag gaacacgtag ccgaccatcg
     2821 ccgggttgaa caggttcttg ccgagccccc cgtagagctg cttcgcgagc gtgatcgcga
     2881 tcgcgccgcc gatcagcggg atccaaaacg gcacgaacgg cggcagcgcg aacgagagca
     2941 gcgccgccgt cacgagcgcg ctgcagtcgc gcagggcgtg gcgcgtcggc cggccgcgga
     3001 gccgcagcac agcgccctcc gtgacgagcg ccgcgcaagc cgtcaacgcg aagttcacga
     3061 gcaggcccgg gccgaagtac cacgtgtggc acagcatcgc cggcacgagc gcgaacagga
     3121 cacgctgcat gatcaccggc acgctgacga gcggccgtgc gtgcggggcc gtcgattggt
     3181 cgaagtgcat ctagtcctgt cccggcgagg tgacggggat cgactggccc gcggcgaccg
     3241 agcgcttggc gatgcggaat cgctccgtca gcatgatctg cgaggggcag atgacgtcgc
     3301 agcagccgca ctcgatgcaa tcctgaagcc cgagcgtgtg cagcgccgcg aagtcgctcg
     3361 tcgtcgcggc cacgagcagg tcctgcggca ggagccgcga ggggcagacc atcgcgcagt
     3421 cgccgcagcg aatgcacggc cactcgtgca tgtccgtcct gacctcgtcg gcgagagcag
     3481 cgatgatgca gttcgtcgcc ttcgtgatcg gcgtctcgtc gttgggcagc gcgtagccca
     3541 tcatgctgcc gccggcgatc aggcgcgcga caccttcgtc gtagccgccg cagagcgcga
     3601 tcagctccgc gatcggcgtg ccgatcggcg cctcgacgtt ctgcggcctc ttgacgccgc
     3661 cgcccgtcac cgtgacgacg cgcgtcacga gcggctcgcc gagcgcgcgc aagcgctgca
     3721 cggcgtatgc cgtgccgacg ttctggcaca cgtagccgaa ctcgccggga taacggccgc
     3781 tcggcacttc ctcgccggtc aggagctcga cgagctgctt ctcgccgccc gcgggataga
     3841 tcgtgggcac ctcggcgagc ttgagcctcg cgtcgtcggc cgcttgcgcc gccgcttcga
     3901 tggcctccat ggcctgcggc ttgtcgcgct cgatcgcgat gatcgcgcgc ggcgccgcga
     3961 gcacgtccgc gaggatcagc gttcccgcga cgatctcggc ggcgttttcg cgcatcagca
     4021 tgtcgtcgca gctgatgtag ggctcgcatt cggcgccgtt gacgatcagt gtttgaacgg
     4081 gcacgggtgt cgcgagcttg tcggccgtcg gatagacggc gccgccgagg ccgacgatgc
     4141 cgccggcacg aatcgcgtcg cgctgctcgg ccaagctcga gggccactcc ggcgcggccg
     4201 cggcgggcac gcgctcgtcg cgcccgtcgc tctcgatgac gatgcagggc gacagctgca
     4261 cgccgtggcc gccgagcgcg ggccgttcct cgatcgcgag gacgaatccc gaagtccccg
     4321 cgtggacggc cgcggaggga gccgccgtgg ccaccgcaac ggtttggccc ttcagcacgc
     4381 ggtcgccgac ggcgacgacg ggctcggccg cgttgccgcg gtgctgattg agcgcgagca
     4441 cgagcttcgt cggcacgaag ctgcgatgga tcggcgacga cgtcgagctc gtcttgttcg
     4501 cgggcagcag aatgccgccg ctcaggtgcc gctcgaagac gtagtcgacc ataacggatc
     4561 agagcctggc gaagcccgcg aaggccatga acgcgagtga caggatgccg gccgtgatga
     4621 atgtgatcgg cgcgccgcga aacgcgatcg gcacgtcggc gagcacgacg cgctcgcgta
     4681 acgccgcgaa cagcgtcagc gcgagcccga agccgacggc cgctccggtg ccgtagaaca
     4741 gtgcggcgac gaagctgcgg ctcgcagccg tgttgatcaa tgccacgccg agaaccgcgc
     4801 aattcgtcgc gacgagcggc acatagacgc cgaggacgcg ctgcaggatg ggcgaaaacc
     4861 gccggatcgc gagatcgatc acgccgacga cgacgccgat cacgagcagc aacgcgaaca
     4921 agcgcagata cgagagctcg taggggcgca aaagatagcg gtccacgagc tgcgtggccc
     4981 cggccgcgat ggtcagcacc aggcctaagc ttaagctcat gccgagcgcg ctttcgacgc
     5041 gctgggatcc cgcgatgaac ggacaaacgc cgagcatctg cacgaggacg aagttattg
//
LOCUS       DASSNN010000093         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_524406, whole genome shotgun sequence.
ACCESSION   DASSNN010000093 DASSNN010000000
VERSION     DASSNN010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524406"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFL84_01715"
     CDS             complement(<1..736)
                     /locus_tag="VFL84_01715"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7607364.1"
                     /translation="MCDQDHFYEDLKKYTRRDVGTFAAAIGVVAMLPRSAFAVDTKES
                     NVTIKTPDGECDAYFVTPTTGAHAAVLVWPDIFGLRPALREMGKRLAGEGYSVLVVNP
                     FYRQQKAPTAPQGAQTPIQQVLPMMQALNATTNTTDAKAFIAWLDGRSEVDKRKKIGT
                     IGYCMGGPIVVRTAAAVPDRVGAVGSFHGGNLTTMGADSPHLLIPKTKAQYLIAIAQG
                     DDERQPESKDILKKTFADAKLQAEVEV"
     gene            926..1318
                     /locus_tag="VFL84_01720"
     CDS             926..1318
                     /locus_tag="VFL84_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HET7607365.1"
                     /translation="MNVASPEGQRMEAHLRKRFGEVWTDIRRELCKHDQQQYADLVQG
                     AGDPEDAATADVLVDLNLAEINRDADELKAIQDALARLKRGEYGYCQRCGEEISPRRL
                     EAIPHATLCIDCQERIERVQAADPTPSL"
     gene            1359..>2020
                     /locus_tag="VFL84_01725"
     CDS             1359..>2020
                     /locus_tag="VFL84_01725"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7607366.1"
                     /translation="MHRSIARPCVGLLTGVAASLSGLPLLAHHSTAEYDAAKLVEARG
                     EVVSVIWRNPHVRFQVSTKAVDGNEQLWDIESADLTRLDRAGLPRDLLKVGDVVSFAG
                     NPSTRTERRMYVTNLLVRDGREILLRGNVQPRWPNASAVGTDVTFAREASAPTTGGNF
                     FGKVLVPMREGRAPEWVRNPPLTAAAREGKAKYDEVVDDPVLGCVSPGMPRVMFRSGP
                     YAV"
BASE COUNT          324 a          693 c          669 g          334 t
ORIGIN      
        1 agacctcgac ctcggcctgc aacttcgcgt cggcgaacgt cttcttgagg atgtccttcg
       61 actccggctg gcgctcgtcg tcgccctgtg cgatcgcgat caggtactgc gccttcgtct
      121 tcgggatcag aaggtgcggg ctgtccgcgc ccatcgtcgt gagattgccg ccgtggaacg
      181 agccgaccgc gccgacgcga tcgggcacgg ccgcggctgt gcggaccacg atcggaccgc
      241 ccatgcagta accgatcgtg ccgatcttct tgcgcttgtc gacctcggag cgcccgtcga
      301 gccacgcgat gaacgccttc gcgtccgtcg tgttcgtcgt cgcgttcagc gcttgcatca
      361 tcggcagcac ctgctgaatc ggcgtctgcg cgccctgcgg cgccgtcggc gccttctgct
      421 ggcgatagaa cggattcacg acgagcacgc tgtagccttc accggccagg cgcttgccca
      481 tctcccgcaa tgccgggcgc agcccgaaga tgtcgggcca cacgagcacg gccgcgtgcg
      541 cgcccgtcgt cggcgtgacg aagtacgcat cgcactcgcc gtccggcgtc ttgatcgtga
      601 cgttgctttc cttcgtgtcg acggcaaacg ccgaccgcgg cagcatcgcc acgacgccga
      661 tggccgccgc gaacgtgccg acgtcacggc gcgtgtattt cttcaggtct tcgtaaaagt
      721 gatcttgatc gcacatggcg tgctctccct ccggttgttg ttgcggcgcc ggtttcgcgg
      781 cggcctgttg cggaatatac gggaagtgcc ctctacgcgc ccacctgtcg ccggatcgcc
      841 ctaccgctcg gttcggctgc gggcacgtga attgcaagca aacaggggcg cgacggaacg
      901 cgccccgcac ccaaaggaga cggccgtgaa cgtagcatca cccgaaggtc agcgaatgga
      961 agcccacttg cgcaagcgct ttggcgaggt ctggaccgac atccgccgcg agctctgcaa
     1021 gcatgaccag caacagtacg ctgacctcgt tcaaggcgcg ggcgaccccg aggacgccgc
     1081 gacggccgac gtgctcgtcg acctgaacct ggccgagatc aaccgcgacg cggacgagct
     1141 gaaagcgatc caggacgcac tggcgcggct caagcgcggc gagtacggct attgccagcg
     1201 ctgcggcgag gagatctcac cgcgccggct cgaggcgatc ccacacgcga cgctctgcat
     1261 cgactgccag gaacgcatcg agcgggttca ggcagcggac cccacgccgt cgctctgagc
     1321 tagaatcagc tcctcgcacg cgtgcggggg aagcgcgtat gcatcgcagt atcgcccggc
     1381 cctgcgtcgg gctcctgacc ggcgtagccg cgagcctgtc cggcttgccg ctgctcgcgc
     1441 atcattcgac ggccgagtac gatgccgcca agctcgtcga agcgcgcggc gaggtcgtca
     1501 gcgtcatctg gcgcaatccg cacgtgcgct tccaagtcag cacgaaagcg gtcgacggca
     1561 acgagcagct ctgggacatc gagagcgccg atctgacgcg gctcgatcgc gcgggattgc
     1621 cgcgcgatct gctgaaagtc ggcgacgtcg tctcgttcgc gggcaatccg tccacgcgta
     1681 cggaacggcg catgtacgtc acgaatctgc tcgtgcgcga cgggcgcgag attttgctgc
     1741 gcggcaacgt gcagccgcgc tggccgaacg cgagcgccgt cggcaccgac gtgactttcg
     1801 cgcgcgaggc ttcggcgcca acgacgggcg ggaatttctt cggcaaggtg ctcgtcccga
     1861 tgcgcgaggg ccgcgcgccc gagtgggtgc gcaacccgcc gctgacggcg gccgcgcgcg
     1921 aaggcaaagc caagtacgac gaggtcgtcg acgaccccgt gctcggctgc gtgtcgcccg
     1981 gcatgccgcg cgtgatgttt cgcagcgggc cgtacgccgt
//
LOCUS       DASSNN010000094         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1406620, whole genome shotgun sequence.
ACCESSION   DASSNN010000094 DASSNN010000000
VERSION     DASSNN010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406620"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFL84_01730"
     CDS             <1..339
                     /locus_tag="VFL84_01730"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET7607367.1"
                     /translation="FERRRQLARLKERIAAIPERPIVVLGDFNDFPGRGLAERTLSPP
                     LTSTPELPTYPSRLPLLPLDRIWFSAPLELTTIATLRDAGRASDHLPLLASLCWPDAP
                     EVGTGFALHN"
     gene            501..1691
                     /locus_tag="VFL84_01735"
     CDS             501..1691
                     /locus_tag="VFL84_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET7607368.1"
                     /translation="MLALILANGRGRALEGLTAWQTKAAVPFGGQYRIVDFVLSNCVN
                     SEIRSIALLTQYKSQSLIRHIHSGWGFLHRELGEFIEIWPAQQQDGERWYRGTVDAVY
                     QNSDLIAATGASDVLVLGCDQLYALDYGPLIEWHAARGADLTVACAHVPESSEHGDVF
                     VTADGRIEHIEHRPPNATAPRRSSLSVMGACVFRAEFLAECLAQSAEQPATADFARDL
                     VPEAVRKARVFAHICDTEEQPHYWRKLNTVDRYWHANMELLANATAFSLHGEGDWPVF
                     TRHEPLPPARVLADAYVDDAVLSPGSVVAGDVTRSVISTRCRVGTNAVIRESVLLPGA
                     VVGAGCILEKVIVDCDAIIPSNTMLGAKLGADSGYYASPEGIVLATSARPASPIDIAA
                     RAIA"
     gene            1703..>3129
                     /gene="treZ"
                     /locus_tag="VFL84_01740"
     CDS             1703..>3129
                     /gene="treZ"
                     /locus_tag="VFL84_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HET7607369.1"
                     /translation="MSAHDMPFGAALTPDGARFAIWAPSVRGAEVEVDGRRVPMRRGA
                     QGFFHCFDTQASAGSRYAFRFDDLGLAFPDPASRFNPDGVHEPSEVVDAAAYAWRDGD
                     WRGHPFHDAVIYELHVGTFTPQGTYSAAAERLDYLVSLGVTAIELMPLAATPGRWNWG
                     YDGVLPFAPHAPYGRPDDLKRFVDAAHERGLMVLLDVVYNHFGPEGNYLGHYAQEFFT
                     ARHQTPWGNGINFDGRSSAIVRQFFIHNALYWLEEYHFDGLRLDAVHAIIDDSDPSFL
                     KELAAAVHAMPSLRPLHLVLENDRNEAELLARDAARRPLVYTAQWNDDFHHALYALLT
                     GETRGHYVDYAEPGAQLLRALREGFVYQGERSLHRGDARGSFSAELPPEAFVNFLQNH
                     DQIGNRPDAARMWALLEPPRMLAAETLLALLPTPILLFMGDEFHAPSGFPFFCDFGEE
                     LARAVTEGRRTEFAGLWEDTAADAVP"
BASE COUNT          471 a         1119 c         1011 g          528 t
ORIGIN      
        1 ttcgagcgcc ggcgccagct cgcgcgcttg aaggagcgga tcgcggcgat cccggagcgc
       61 ccgatcgtcg tgctcggaga cttcaacgac ttcccgggcc gcgggctcgc cgagcgcacg
      121 ctgtcgccgc cgctgacctc gacgcccgag ctgcccacct acccctcgcg cctgcccctg
      181 ctcccgctcg accggatctg gttcagcgcg ccgctcgagc tgacgacgat cgcgacgctg
      241 cgcgacgcgg gccgcgcctc ggaccatctg ccgctgctcg cgagcttgtg ctggcccgac
      301 gcgcccgaag ttggcacggg gtttgcttta cacaattaga gaatttcaac gcgggccgcc
      361 cgaatgggta attccgggcg gcattttagg gggtcgcttt acaaaccttg taattcgacc
      421 cccgtttttt ccaagtttct gcaagggggg ccttcatgct tcgggccggc cgcctcgccc
      481 cgagccgggg atgcgaaggc gtgctcgcac tgatcctcgc gaacggccga ggccgggcgc
      541 tcgagggcct aacggcctgg caaacgaagg ccgcggtgcc gttcggcggg cagtatcgca
      601 tcgtcgattt cgtgctctcg aattgcgtca actccgagat ccgcagcatc gcgctgctga
      661 ctcagtacaa gtcgcagtcg ttgatccgcc acatccactc cggctggggc ttcctgcacc
      721 gcgagctcgg ggaattcatc gagatctggc ccgcccagca gcaggacggc gagcgctggt
      781 accgcggcac cgtcgacgcc gtctaccaga acagcgacct cattgcggcc acgggagcga
      841 gcgacgtgct cgtgctcggc tgcgaccagc tctatgcgct cgactacgga ccgctcatcg
      901 aatggcatgc cgcccgcggg gccgacctga ccgtcgcctg cgcgcacgtt cccgaaagca
      961 gcgagcacgg cgacgtcttc gtcaccgccg acggccgcat cgagcacatc gagcaccggc
     1021 cgccgaacgc gactgctccg cgccgctcgt cgctctcggt catgggcgct tgcgtgttcc
     1081 gcgccgagtt cctcgccgaa tgcttggcgc agtcagccga gcagccggcc acggcggact
     1141 ttgcccgcga cctcgtgccc gaagccgtgc gcaaggcgcg agtgttcgcg cacatttgcg
     1201 acacggagga gcagcctcac tactggcgaa agctgaacac cgtggatcgc tactggcacg
     1261 cgaacatgga gctgctcgcc aacgcgacgg cgttctctct gcacggcgaa ggcgactggc
     1321 ccgtgttcac tcgccacgag ccgctgcctc cggcgcgcgt gctcgccgac gcctatgtag
     1381 acgacgccgt gctgagcccg ggctcggtcg ttgcgggcga cgtcacgcgc tcggtcatct
     1441 cgacacgctg ccgcgtcggc accaacgccg tgattcgcga atcggtgctg ttgccgggcg
     1501 ccgtcgtcgg cgccggctgc atcctcgaaa aggtcatcgt cgactgcgac gccatcattc
     1561 cgagcaacac gatgctcggc gcgaaattgg gcgcagactc gggttactac gcctcgcctg
     1621 aaggaatagt gctcgccacg tcggcgcggc cggcgtcgcc catcgacatt gccgcacgcg
     1681 caatcgctta gcgcgcgcga tgatgagcgc ccacgacatg ccgttcggcg cggcactcac
     1741 gcccgacggg gcgcggtttg cgatctgggc gccttccgtg cgcggcgccg aagtcgaggt
     1801 cgacggccgg cgcgtgccga tgcggcgcgg cgcgcagggg ttctttcact gcttcgatac
     1861 gcaggcctcc gccggctcgc gctacgcgtt tcgcttcgac gacttggggc tcgccttccc
     1921 cgacccggct tcgcgcttca atcccgacgg cgttcacgag ccttccgagg tcgtcgacgc
     1981 ggccgcttac gcgtggcgcg acggcgactg gcgtggccac ccgttccacg atgccgtaat
     2041 ctacgagctg cacgtcggca cgttcacgcc gcagggcacc tattcggcgg ccgccgagcg
     2101 cctcgactac ttggtctcgc tcggagtcac ggcgatcgag ctcatgccgc tcgctgcgac
     2161 gccgggacgc tggaactggg ggtacgacgg cgtgctgcca ttcgcgccgc atgcgcctta
     2221 cggccggccc gacgacttga agcgcttcgt cgacgctgcg cacgagcgcg gcctcatggt
     2281 gctgctcgac gtcgtctaca accacttcgg tcccgaggga aactacctcg gccactacgc
     2341 gcaagagttc ttcacggcgc gtcatcagac cccgtggggc aacgggatca acttcgacgg
     2401 ccgctcgagc gcgatcgtcc gccagttctt catccacaac gcgctgtact ggctcgagga
     2461 atatcacttc gacgggctgc gcctcgatgc cgtccacgcg atcatcgacg actccgatcc
     2521 gagcttcttg aaggagctgg ccgcggccgt gcacgccatg ccgtcactta ggccgctgca
     2581 cctggtcctc gagaacgacc ggaacgaagc cgagctgctc gcgcgcgatg cggcgcgacg
     2641 gccgctcgtg tatacggcgc aatggaacga cgacttccat cacgcgctgt acgcgctgct
     2701 gaccggagag acgcgcggtc attacgtcga ttatgccgaa ccgggcgcac agctgctgcg
     2761 cgcgctgcgc gagggcttcg tgtaccaggg cgagcggtct cttcaccgcg gcgatgcacg
     2821 cggcagcttc agcgccgagc tcccgccgga ggccttcgtc aatttcctcc aaaaccacga
     2881 ccagatcggc aaccggccgg acgccgcgcg catgtgggcg ctgctcgagc cgccgcgcat
     2941 gctcgcggcc gagacgctgc tcgcgctgct gccgacgccg atcctgctat tcatgggcga
     3001 cgagttccat gcgccgagcg gcttcccgtt cttctgcgat ttcggcgaag agctcgcccg
     3061 cgccgtgacg gagggccgcc gcaccgagtt cgcgggtctg tgggaggaca ccgccgcgga
     3121 cgccgtgcc
//
LOCUS       DASSNN010000095         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1406670, whole genome shotgun sequence.
ACCESSION   DASSNN010000095 DASSNN010000000
VERSION     DASSNN010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406670"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFL84_01745"
     CDS             <1..444
                     /locus_tag="VFL84_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7607370.1"
                     /translation="VSTSSEAHRRAKLDFDDLQSERSYSGFRVYGRSKLANILFTREL
                     ARRLQGSGVNANCLHPGFVATRFGEGNGGLGRLALHAAQRAFAITPEEGAKTMTYLAS
                     SPEVAEVSGKYFYKCKEELPSAEAQDDVAAARLWAESAKLSGVGT"
     gene            complement(771..2408)
                     /locus_tag="VFL84_01750"
     CDS             complement(771..2408)
                     /locus_tag="VFL84_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7607371.1"
                     /translation="MLYYAAPLRSDRNQLIGANVLLIDVTEREEAQRIAREELQGSEA
                     TFRSFFDSVAVGVALVRVGGRYLNVNDRYCEITGYSREELLNMSPYELDHPEERAEDR
                     RRVHEALANPSGIYCAEKRYVRKDGSIRWVLVAANFLRDERQRPVHTVAVCLDITQRK
                     RAEEALHESDRLKDEFLATLAHELRNPLAPLKNAAELLSSADAREASWCRDVIDRQVG
                     HMTRLIDDLLDVSRITCGKLELRTARVELRDVINGAIEASRPMLECCEQRLATRLPDD
                     AVYLHGDPVRLIQVFTNLLTNAAKFTETPGSIELSAAADGDEVSISVADTGIGIASDE
                     LSRVFDKFYQSPRRGARFLGGLGIGLSLVQRLVQLHGGSIEARSTGLGRGSEFVVRLP
                     LAQAHTQAEEPPTEEASPERDARRGIRILIVDDNIDGADALARLLATMGHETVTEYEG
                     QGALKRAAEFSPDFIFVDLGMPGMDGFELARRLRALSPRYRPRIVALTGWGREEDIAR
                     TSQAGFDAHLVKPVERTVLERQLNERTGSGPATGAAA"
BASE COUNT          407 a          776 c          778 g          450 t
ORIGIN      
        1 gtgtcaactt cgtccgaagc gcacaggagg gccaagctgg acttcgacga tctgcagtcg
       61 gagcgcagct attccggttt tcgcgtctat ggcaggtcca agctcgccaa catcctgttc
      121 actcgtgaac tcgctcggcg cctccaaggc tccggcgtga atgctaactg tttgcatccg
      181 ggtttcgttg ccacacgctt cggcgagggc aacggaggct tgggccgttt agcactgcac
      241 gcggcgcagc gcgcctttgc aattacgccg gaggaggggg ctaaaacgat gacttatctc
      301 gcttcctcgc cggaagtcgc cgaggtcagc ggcaagtatt tttacaagtg caaggaggaa
      361 ctaccgagtg ccgaagccca ggacgatgtg gccgcagcgc ggctttgggc ggaatcggct
      421 aagctctccg gcgtcggaac gtaagtgacg tgaacggtga cggatgaaga tcaaattcat
      481 gtgggcgcaa ctccgcgtca cgatcacgcc gtccggcggc gcaaagatga ctcggtccgg
      541 cacaccctga ccattctgag gaaggaaaac ggccgatggc gcttggcccg cgacgcgaat
      601 ctgctcgggc cgccgatgca gcaatagcgc ggccgttccc gttgtagacg acaccgcttc
      661 ggcccgctca tggcgttctt atcatgctgt cgcgtgaacg atttcgcgat cgaaaatcgt
      721 gcggtctggc tcccctgggc cggtacgcct cggcggtcga tccgttcccg ctaagccgcg
      781 gcgcccgtcg ccgggcccga ccctgttcgc tcattcagct ggcgttccag gactgtgcgt
      841 tccaccggct tcacgaggtg cgcgtcgaag ccagcctgcg acgtgcgggc gatgtcctct
      901 tcgcgccccc agccggtcaa ggcgacgatg cgcggccggt agcgcggcga cagcgcgcgg
      961 aggcgccgcg cgagttcgaa gccgtccatg ccgggcatcc cgagatcgac gaagatgaag
     1021 tcgggactga attccgcggc gcgcttgagt gcgccttggc cttcgtattc cgtcaccgtc
     1081 tcgtgtccca tcgtcgcaag caggcgggcc aacgcatccg cgccgtcaat attgtcgtcg
     1141 acgatgagga tccggatgcc gcggcgcgcg tcgcgctcgg gggatgcttc ttccgtaggc
     1201 ggctcttcgg cctgcgtgtg agcttgcgca agcggcagcc gcaccacgaa ttcgcttccg
     1261 cggccgagcc ccgtgctccg cgcttcgatg ctgccgccgt gcagctggac gagacgctgc
     1321 acgagcgaga gcccgatgcc gaggccgcct aagaaccggg cgccgcgccg cggactctga
     1381 tagaacttgt cgaagacgcg cgaaagctca tcgctcgcaa tgccgatgcc ggtgtctgcg
     1441 acgctgatgc tgacctcatc gccatcggct gccgcggaga gttcgatcga tcccggcgtc
     1501 tcggtgaact tcgcggcgtt ggtcaggaga ttcgtaaaca cttggatcaa gcgaaccggg
     1561 tcgccatgga ggtacacggc atcgtcggga agccgagtgg ccagcctctg ctcgcagcac
     1621 tcgagcatcg gccggctcgc ctcgatcgcg ccgttgatca cgtcgcggag ctcaacgcga
     1681 gccgtgcgca gctcgagctt gccgcacgtg atacgggaga cgtcgagcaa atcatcgatg
     1741 aggcgcgtca tgtgaccgac ctgacgatcg atcacgtcgc ggcaccacga tgcctcgcgc
     1801 gcgtcggcgc tggaaagcag ctcggccgca ttcttgagcg gtgcgagcgg gttgcgcagc
     1861 tcgtgcgcga gcgtcgcgag aaattcgtcc ttgagtcggt cggattcgtg cagcgcctct
     1921 tcggcgcgct tgcgttgagt gatgtccagg caaacggcaa cggtgtgaac cgggcgctgc
     1981 ctctcatctc tgagaaaatt ggctgcgacc agcacccagc ggatgctgcc gtccttgcgc
     2041 acgtaccgct tctcggcgca atagatgccg gaagggttcg ccaacgcctc gtgcactcga
     2101 cggcggtctt ccgcgcgctc ttccggatgg tcgagctcgt acgggctcat gttcagcagc
     2161 tcctcgcggc tatagcccgt aatctcgcaa tagcggtcgt tgacgttcag atagcggccg
     2221 ccgacgcgca cgagcgccac tcctactgct acgctgtcga agaagcttcg aaaggtggcc
     2281 tcgctgccct gcagctcctc gcgcgcgatg cgctgcgcct cctcgcgctc ggtcacgtcg
     2341 atgagcaaga cgttcgctcc gatcaactgg ttgcgatcgc ttcgcagcgg cgccgcgtag
     2401 tagagcacgc t
//
LOCUS       DASSNN010000096         2518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_398412, whole genome shotgun sequence.
ACCESSION   DASSNN010000096 DASSNN010000000
VERSION     DASSNN010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398412"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..299)
                     /locus_tag="VFL84_01755"
     CDS             complement(135..299)
                     /locus_tag="VFL84_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA binding domain-containing
                     protein"
                     /protein_id="HET7607372.1"
                     /translation="MAEERKSFLLRLPPELWKEIERWAADELRSVNGQIEYVLRQAVA
                     RRKNDSTENE"
     gene            complement(311..1309)
                     /locus_tag="VFL84_01760"
     CDS             complement(311..1309)
                     /locus_tag="VFL84_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002563092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET7607373.1"
                     /translation="MAVPPSQHTVERERQALNGWLALALVVLWFIAAIAVFLSIGSID
                     DPEGNPGAVALRIVGAVLMIGLGIFCCFGFFTLEPNEARVLILFGAYKGTVRVSGFHW
                     ANPLFARHRGVVPGSSPVATKSPHGGTRLTQPKLPTKLSLRVHNFNSQTLKVNDKRGN
                     PVEIAAVVVWRVDDTAQAVFDVEDYESYVQVQSEAAIRGIASLYAYDHGEEHELTLRG
                     GGDEVAHALKKELEVRLAKAGVVVEEARLTHLAYAPEIAPAMLRRQQAEAVIAAREKI
                     VLGAVSMVEMALDELARKQVVKLDEERKAAMVSNLLVVLCGTEEASPVINTGTLYS"
     gene            complement(1425..1847)
                     /locus_tag="VFL84_01765"
     CDS             complement(1425..1847)
                     /locus_tag="VFL84_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607374.1"
                     /translation="MTGAGAARATLVAAIVANGLGWVLPAFSDERGWAAFFFALSPLW
                     DYRSFQSEPFPLLAFIVSSALTNVLFVALAVALALGKTRLAKPVLWAAAAATLLNLYW
                     PILLEADRARLETGFFIWVSSFALLALSAFLTLRSAPR"
     gene            complement(1844..>2518)
                     /locus_tag="VFL84_01770"
     CDS             complement(1844..>2518)
                     /locus_tag="VFL84_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7607375.1"
                     /translation="PRGYFPKIQAVLEKHDVLLIDDEVITGFGRTGELWGAQAFAMRP
                     QTLSAAKALSSAYLPISAVVVPEFLYAPMIEASGEVGLFGHGFTYSGHPVAAAVALRA
                     LGIYEERKLYAHVRHIAPQFQAALASLGSHPLVGDTRGIGLVGACELVKDKGSKAAFD
                     AKLAVGAKCMQLCQEHGLIVRAIGDVIALCPPFIVTPSEVDEIFAKLARGLDDTLAWA
                     KREKLK"
BASE COUNT          439 a          865 c          824 g          390 t
ORIGIN      
        1 cgacgagctt catgacgtca cctctcggtt gtgtttgaag ggaccgaacc agcgcggcac
       61 tcgcgcctcg tagcgaggcc aatcgtccca tgggcttcgc gaaaagccat agagccccga
      121 tccgcacccc cctttcactc gttctcggtc gaatcgttct tgcgccgcgc gacggcctga
      181 cgtagcacgt actcgatctg accgttgacg ctgcgcagct cgtctgccgc ccatcgctcg
      241 atctctttcc agagctcggg cggcagccga agcaagaacg acttgcgctc ttcagccacg
      301 acgcgggccg tcagctatac agcgtgccgg tgttgatgac gggcgaagcc tcctcggtgc
      361 cgcagagcac gacgagcaag ttgctgacca tcgcggcctt gcgctcctcg tcgagcttga
      421 cgacttgctt gcgcgcgagc tcgtcgagcg ccatctcgac catgctgacg gcgccgagca
      481 cgatcttctc gcgcgccgcg atgacggctt cggcttgctg acggcgcagc atcgccggcg
      541 cgatctcggg cgcgtacgcg aggtgcgtga gcctcgcttc ctcgacgacg acgccggcct
      601 tcgccaaacg cacctcgagc tccttcttca aggcgtgcgc gacctcgtcg ccgccgccgc
      661 gcagcgtcag ctcgtgctcc tcgccgtggt cgtaggcgta gaggctcgcg atgccgcgga
      721 tcgcggcctc gctctgcacc tgcacgtagc tttcgtagtc ctcgacgtcg aacacggcct
      781 gggcggtgtc gtcgacgcgc cagacgacga cggccgcgat ctcgacggga ttgccgcgct
      841 tgtcgttgac cttcagcgtc tggctgttga agttgtgcac gcgcagcgag agcttcgtcg
      901 gcaacttcgg ttgcgtcagc cgcgtgcctc cgtgcggcga cttcgtggcc acgggcgacg
      961 agcccggcac cacgccgcgg tgacgcgcaa agagcggatt ggcccagtga aacccgctca
     1021 cgcggaccgt tcccttgtag gcgccgaaca ggatcagcac gcgcgcctcg ttcggctcga
     1081 gcgtgaagaa cccgaagcaa cagaagatcc caagcccgat catcaacacc gcacccacga
     1141 ttcgcagggc gacggcacca gggttgcctt ccgggtcgtc aatcgacccg atcgagagaa
     1201 acacggcgat cgcggcgatg aaccacagca cgaccagcgc caacgccaac cagccgttga
     1261 gggcctgccg ctcgcgctcg accgtgtgtt gggacggggg aacagccatg ggtcgcacct
     1321 cttggtattg atatgacagt gatatcacca cgaaatcacg cggcgcgtca atgcctggcc
     1381 gcgcgacgcg cgctccaagg gttcctacga ccggcggatt tgacttaacg gggcgccgag
     1441 cgaagcgtga gaaacgccga cagcgcgagc agcgcgaacg acgagaccca gatgaagaag
     1501 cccgtctcga gccgggctcg atccgcctcg agcaggatgg gccagtacag attcagcagc
     1561 gtggccgccg ccgcggccca cagcacgggt ttggcgaggc gcgtcttgcc gagcgcgagc
     1621 gcgaccgcga gcgcgacgaa caacacgttc gtcaacgcgc tcgagacgat gaatgcgagc
     1681 agcgggaacg gctcgctctg aaagctgcgg tagtcccaga gcgggctcag cgcgaaaaag
     1741 aacgcggccc agcctcgctc gtcggagaag gccggcagca cccagccgag cccgttggcg
     1801 acgatcgcgg cgacgagcgt cgcgcgcgcc gcgccggcgc cggtcacttg agcttctcgc
     1861 gcttcgccca cgcgagcgtg tcgtcgaggc cgcgcgcaag cttcgcgaag atctcgtcga
     1921 cctcggacgg cgtcacgatg aacggcgggc acagcgcgat gacgtcgccg atcgcgcgca
     1981 cgatcaagcc gtgctcctga cagagctgca tgcatttggc gccgaccgcg agcttggcgt
     2041 cgaacgcagc cttgctgccc ttgtccttga cgagctcgca ggcgccgacg agcccgatgc
     2101 cgcgcgtgtc gccgacgagc ggatgcgagc cgagcgacgc gagcgcggcc tggaactgcg
     2161 gtgcgatatg ccgcacgtgc gcgtagagct tgcgctcctc gtagatgccg agcgcgcgca
     2221 gcgccacggc ggccgcgacg gggtgccccg aatacgtgaa gccgtgcccg aacaagccga
     2281 cctcgccgct ggcctcgatc atcggcgcgt agaggaactc gggcacgacg acggcgctga
     2341 tcggcaggta cgccgacgac agcgctttgg ccgccgacag cgtctgcggt cgcatggcga
     2401 acgcttgcgc gccccaaagc tcgcccgtgc ggccgaagcc cgtgatgacc tcgtcgtcga
     2461 tcaagagcac gtcgtgcttc tcgagcacgg cttgaatctt cgggaaatag ccgcgcgg
//
LOCUS       DASSNN010000097         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_524740, whole genome shotgun sequence.
ACCESSION   DASSNN010000097 DASSNN010000000
VERSION     DASSNN010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524740"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1823
                     /locus_tag="VFL84_01775"
     CDS             <1..1823
                     /locus_tag="VFL84_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7607376.1"
                     /translation="TGTVAMEHEELLSVSRGAGLGAIATFVMVAVVLYAALRSWRLLA
                     ASIVTLIAGLALTAAFAAVTVGKLNLLSIAFVVLNVGLGSDYVIHVLLRYRELIAQGL
                     ATNAAVVEAVRDVGSSLVLCAFTTAAGFYAFIPTTFSGVSELGLIAGTGVFFGLFASV
                     TLLPALVACGKSDARGLGFAPTWLDPRIFAPLSRRPRLVLGVTTVVVAAAFAALPRVT
                     FDSNPIHLRDPGSESVRTLLDLAAAGDAPLLNLVAVAPDAAAARRWAADLEKLREVRA
                     VTTTDSLVPAEQDEKLALLEDLALLMGGGFGEIERTPADTSALARSLAALEAASADVP
                     AAHALHDAVAKWLDGGDAGDHEAALSRLDEALTVGLPRELERLAAALQAERFDRGDLP
                     QALAQRWVAADGRELVEIAPVEDVSDNAAARRFIGAVRTVVPTATGLPVVYQEASATV
                     VSAFERALTYAFILVAAIIWLVLRRLKDTLLVLVPIALATVLTAALTLVVGMPFNYAN
                     IIALPLLVGMGVDNGIHVVHRMRNETVDRLFDTSTMRAVLASALTTVASFGNLAFSSH
                     RGTASMGVLLALGLVTSMAVTLIVLPAWLTLRSPQTRVAA"
     gene            1820..2806
                     /gene="hpnA"
                     /locus_tag="VFL84_01780"
     CDS             1820..2806
                     /gene="hpnA"
                     /locus_tag="VFL84_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HET7607377.1"
                     /translation="MTALVTGANGFLGSAVVRALLADGSAVRALVRAGSDRRNLDELD
                     VEVVPGDLTDRDSLRRAAAGCDSVFHVAADYRLWVADPKPMYRANVDGSVNVLEAAAA
                     AGATRMVYTSSVAVLGINKDRTPADEDTPVTVDDMVGHYKRSKFLAEQAVRARAAELE
                     FPVVTVNPSTPIGPRDVKPTPTGRILLDAAAGRMPAFVDTGLNLVHVDDVAAGHLAAL
                     KRGSPGERYILGGDDFTLQQILELVAEHVGRRASTIRLPHWSVYPLAVGAEAVAMLTK
                     REPRVTLDGVRMSKKHMYFSSGKAQRALGYRWRDPRLGLVAAVDWFKANGYF"
     gene            2793..>3603
                     /gene="mvaD"
                     /locus_tag="VFL84_01785"
     CDS             2793..>3603
                     /gene="mvaD"
                     /locus_tag="VFL84_01785"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET7607378.1"
                     /translation="MATSKHDGRATARAGANFALVKYWGKADARLNVPAVGSISITLD
                     SLFTETEVALAPALRSDELVLDGKRRDEDVAKITACVDLLRQKAGVDTRVRVASRNNF
                     PTGAGLASSASGFAALVRAAEAAFGLALTPRERSIVARQGSGSAARSVFGGFVEMHAG
                     TATDGSDSYAEPLLEGAEWPLEVVIAVTAKGEKEIGSRSGMTRSAASSPYYAAWVAGQ
                     PPDLAAARAAIRARDFAALADVAEHNCLKMHAAALAAQPPLVYWNGATVECL"
BASE COUNT          498 a         1244 c         1253 g          608 t
ORIGIN      
        1 tcacgggcac ggtggccatg gagcacgaag agctcctgag cgtgagccgc ggcgccgggc
       61 tcggcgccat cgccacgttc gtcatggttg ccgtcgtgct ctatgcggcg cttcgctcgt
      121 ggcggttgct cgccgcgtcc atcgtgacgc tgatcgccgg cctggcgttg acggccgcgt
      181 tcgccgccgt caccgtcggc aagctgaatt tgttgtcgat cgcgttcgtc gtgctcaacg
      241 tcggcttggg cagcgactac gtcattcacg tgctgttgcg ctatcgcgag ctcatcgcgc
      301 aagggctcgc gacgaacgcg gcagtcgtcg aagccgtgcg cgacgtcggc tcgtcgctcg
      361 tgctgtgcgc attcacgacg gcggccgggt tctatgcgtt catcccgacc acgttctccg
      421 gagtatccga gctcggcctg atcgcgggca cgggcgtgtt cttcggtctg ttcgcgagcg
      481 tgacgctgct gccggcgctc gtcgcctgtg ggaaaagcga tgcgcgtggg ctcggctttg
      541 cgccgacctg gctcgatcca cgaatcttcg cgccgttgtc gcggcggccc cggctcgtgc
      601 tcggcgtgac gacggtcgtc gtcgccgcgg ccttcgccgc tctgccgcgc gtgacgttcg
      661 acagcaatcc gatccacttg cgcgatcccg gcagcgagtc cgtgcgcacg ctgctcgatc
      721 tcgccgcggc cggcgatgcg ccgcttctga atctcgtcgc ggtcgctccc gatgcggcag
      781 cggcgcggcg ctgggccgcc gacctcgaaa agctgcgcga ggtgcgcgcc gtcacgacga
      841 ccgacagcct cgtgccggcc gagcaggacg agaagctcgc gctgctcgaa gacctcgcgc
      901 tgctcatggg cggcggtttc ggcgagatcg agcgcacacc cgccgacacg tccgcgctgg
      961 cgcgttcact cgccgcgctc gaagccgcga gcgccgatgt gccggccgcg cacgcgctcc
     1021 acgacgcggt tgccaagtgg ctcgacggcg gcgacgccgg cgaccacgaa gcggctttga
     1081 gccgcctcga cgaggcgctg acggtcggct tgccgcgcga gctcgaacgg ctcgccgccg
     1141 cgttgcaggc cgaacggttc gaccgcggcg atctgccgca ggcgctcgcg cagcgctggg
     1201 tcgccgcgga cggccgcgag ctcgtcgaaa tcgcgccggt ggaggacgtc agcgacaacg
     1261 cagccgcgcg ccgcttcatc ggcgcggtgc gcacggtcgt gccgacggca acgggcttgc
     1321 cggtcgtcta tcaggaggcg tcggcgaccg tcgtctcggc gttcgagcgc gcgctcacgt
     1381 atgcgttcat cctggtcgcc gcgatcatct ggctcgtgct gcgccgcttg aaagacacac
     1441 tgctcgtgct cgtgccgatc gcgctcgcga ccgtgctcac ggccgctctg acgctcgtcg
     1501 tcggcatgcc gttcaattac gcgaacatca tcgcgctgcc gctgctcgtg ggcatgggcg
     1561 tcgacaacgg cattcacgtc gtgcatcgca tgcgcaacga gaccgtcgat cggctgttcg
     1621 acacgagcac gatgcgcgcg gtgctcgcga gcgcgctcac gaccgtggcg agcttcggca
     1681 atctcgcgtt ctcgtcgcac cgcggcaccg cgagcatggg cgtcttgctc gcgcttgggt
     1741 tggtcacgag catggccgtg acgctgatcg tgttgccggc gtggctcacg ctgcgctcgc
     1801 cgcaaacgag agtcgcggca tgactgcgct cgtcacgggc gccaacgggt tcctgggctc
     1861 ggccgtcgtg cgcgcgctgc tcgccgacgg ctcggccgtt cgcgcgctcg ttcgggcagg
     1921 atccgatcgg cgcaatctcg acgagctcga cgtcgaggtc gtgcccggcg atctcacgga
     1981 tcgcgattcg ttgcgccgcg ccgcggcagg ttgcgactcc gtctttcacg tcgcggccga
     2041 ctatcgactc tgggtcgccg accccaaacc gatgtaccgc gcgaacgtcg acggctccgt
     2101 caacgtgctc gaagcggcgg cggcggccgg cgcaacgcgc atggtctata cgtcgagcgt
     2161 cgccgtgctc ggcatcaaca aagaccgaac gccggccgac gaggacacgc ccgtgacggt
     2221 cgacgacatg gtcggccact acaaacgctc gaagttcttg gccgagcaag ccgtgcgcgc
     2281 gcgcgcggcc gagcttgagt tcccggtcgt cacggtcaac ccttcgacgc cgatcggtcc
     2341 gcgtgacgtc aagccgacgc caacgggccg aattctgctc gatgccgccg cgggtcgaat
     2401 gccggcgttc gtcgatacgg gactgaacct cgtgcacgtc gacgacgtcg cggcgggcca
     2461 cctcgcggcg ttgaagcgcg gctcgcccgg cgaacgctat atcttgggcg gtgacgactt
     2521 cacgctgcag cagattcttg agctcgtcgc agagcacgtc ggccggcgag cgagtacgat
     2581 tcgcctgccg cattggtcgg tctatccgct cgccgtgggc gcagaggcgg tggcaatgct
     2641 gacgaagcgc gagccgcgcg tgacgctcga cggcgtgcgg atgtcgaaga agcacatgta
     2701 tttctcgagc ggcaaagcgc agcgagctct cgggtatcgg tggcgcgacc cgcgcttggg
     2761 tctggtggcc gccgtcgatt ggttcaaggc gaatggctac ttctaaacac gacgggcgcg
     2821 ccacggcgcg cgcgggcgcg aacttcgcgc tcgtcaaata ttggggcaag gccgatgcac
     2881 ggctcaacgt gccggccgtc ggctcgatct cgatcacgct cgattcgttg ttcacggaga
     2941 cggaggtcgc gctggcgccc gcgcttcgca gcgacgagct cgttctcgac ggtaagcggc
     3001 gcgacgagga tgtcgcgaag atcaccgcgt gcgtcgactt gctgcggcag aaagccggtg
     3061 tcgacacgcg cgtgcgcgtt gcgagccgca acaactttcc gacgggcgcc gggctcgctt
     3121 cttcggcgtc cggcttcgcc gcgctcgtgc gcgccgccga ggcggcgttc ggtctggcgc
     3181 tgactccgcg cgagcgcagc atcgtcgctc ggcaagggtc gggatcggcc gcgcgctcgg
     3241 tcttcggcgg attcgtcgag atgcacgccg gcacggccac cgacggcagc gacagctacg
     3301 ccgagccgct gctcgaaggc gcggagtggc ctctcgaagt cgtcatcgcg gtcacggcaa
     3361 aaggcgaaaa ggagatcggc tcgcgcagcg gcatgacacg ctccgcggcc tcgtcgccgt
     3421 actacgcggc gtgggtcgca ggccaaccgc ccgatcttgc ggccgcgcgc gccgcgattc
     3481 gcgcgcgcga tttcgcggcg ctcgccgacg tcgccgagca caactgcctc aagatgcacg
     3541 ccgcggcact ggccgcgcag ccgccgctcg tctactggaa cggtgcgacg gtcgaatgcc
     3601 tgc
//
LOCUS       DASSNN010000098         3701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_524770, whole genome shotgun sequence.
ACCESSION   DASSNN010000098 DASSNN010000000
VERSION     DASSNN010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524770"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFL84_01790"
     CDS             <1..372
                     /locus_tag="VFL84_01790"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HET7607379.1"
                     /translation="VGVLGADDVGEALAALTADRTVNGRRIVVRRLLPDDELAGVHVL
                     FFGEAARARADRIASEAAAQNILTVTDFQAGADDSVIEFVTANGKLRFEVRLDTAESN
                     GLKLHAGLLNVAARVHGSSQR"
     gene            369..1850
                     /locus_tag="VFL84_01795"
     CDS             369..1850
                     /locus_tag="VFL84_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7607380.1"
                     /translation="MTSTRHKNSVRRQLLLGVLATTGCALLITGAAVAYFDLRSFRDS
                     ALADLTAMGDILALASTPALEFDDPQTASEYLELLRAKPAITAAALYAPNGALFAKYS
                     SGEATPDFPELPGHDGYQVAGKEIALFKRVIADGEIVGTVYVRAHYDVAGRIERYLGI
                     LAAVLLLSLAAAALMSHRLHRGITEPIRGVVDVARNVLDKRDYSARAPKGHDDELGVL
                     VDAFNNMLDEVGERTAVLERAREELRTLNAELEQRVAARTAQLETANKELEGFSYSVS
                     HDLRAPLRAVGGFAELLRDDHAAQLDAEGLRKLDVIRDEAARMGTLIDDLLAFSRLGR
                     KSLQPAELDMADLVSNVVDRLRADSGGERVAFRMGRLPRAWGDRALLEQVWVNLLSNA
                     VKFSSKKDAPVVEVGAISAEREHVFFVRDNGAGFDPRYASKLFGVFQRLHDGAEFPGT
                     GVGLALVQRIVVRHGGRVWADSKPGEGATFHFTLPKENSHAGV"
     gene            1837..2277
                     /locus_tag="VFL84_01800"
     CDS             1837..2277
                     /locus_tag="VFL84_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7607381.1"
                     /translation="MQEFEHVEILLVEDNPHDAELAIRGLEKHKVANKVTWVKNGEEA
                     LDFVFRRGMFEGRTDAGPRLILLDLKMPKVDGIEVTRAVKADPRTRHIPIVIMTSSQE
                     EKDVANGYSCGANSYIVKPLDFKSLVDVAAQVGFYWLAINRAQP"
     gene            complement(2280..>3701)
                     /locus_tag="VFL84_01805"
     CDS             complement(2280..>3701)
                     /locus_tag="VFL84_01805"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HET7607382.1"
                     /translation="ALLKQVAANITFALQYLRSKENAEYLEYYDTLTGLPNRALYVTR
                     AQAVLDSAKKAGKSVAVAVIDIVELGTVNDNLGQHVGDSLLRLVAERLTHAFGHSTAV
                     SRLGDDKFSVLEIEPVHAEGGLAKRIADCFEAPFSLQSQDLRVDARAGFVEFPDDGDD
                     AEALLQQAKVALQQAKDTGARCLRYNARMNETASQRFLLTNQLRRAVAERGFTLHYQP
                     KLNLKTGGVDGVEALLRWSPDSEPVPPNVFVPMLESLRLIDEVGSWVVVQAMTETAQW
                     ATAADFRVAANVSPLQLNREDFARALLDSIAELGGNPRRLELEVTESTLMADPRHSSV
                     SLAELRAAGVSIAIDDFGTGHSSLRVLAGLPIDVLKIDRSFVSDLVTNRNHRLIVQTT
                     IRLAASLGMKTVAEGVETLEQLELLRALGCDAVQGYFVSRPVTAADLGRWLTGETFGK
                     LKQYAAPRAEKRAKAASRRSRST"
BASE COUNT          636 a         1179 c         1241 g          645 t
ORIGIN      
        1 gtcggcgtcc tcggcgccga cgatgtggga gaggcactcg cggcgctgac ggccgatcgc
       61 acggtcaacg gccgccgcat cgtggtgcgg cggctccttc cagacgacga gctcgcgggc
      121 gtccacgtgc tgttcttcgg cgaggccgcg cgggcgcgcg cggaccgcat tgccagcgag
      181 gccgctgcac aaaacatttt gaccgtgacg gactttcaag cgggagcgga cgatagcgtc
      241 atcgagttcg tcacggccaa cggcaagctg cgtttcgaag ttcgcttgga caccgccgag
      301 agcaatggcc tcaagttgca cgccggtctg ctgaacgtcg cggcacgcgt gcacggctcg
      361 agccaacgat gacatcgact cgccacaaga actccgtccg ccgccagttg ctgctcggcg
      421 tcctcgcgac gacgggctgt gcgctgctga tcacgggcgc ggcggttgcg tatttcgacc
      481 tgcgcagctt ccgcgacagc gcgcttgcag acctaaccgc catgggcgac attctggctc
      541 tggcgagcac gccggcgctc gagttcgacg acccgcaaac cgcaagcgag tatctcgagt
      601 tattgcgcgc caagccggcc attacggccg cggctctgta cgcgccgaac ggcgctctgt
      661 tcgcgaagta ttcgtccggc gaagccacgc cggactttcc cgagctgccc ggccacgacg
      721 gctaccaagt cgccggcaaa gagatcgccc tgttcaagcg tgtcatcgcg gacggcgaga
      781 tcgtcgggac cgtctacgtt cgcgcgcact atgatgtggc cggccgcatc gagcggtacc
      841 tcggcatcct agccgcggtc ttgctgctga gcctcgctgc ggcagcgttg atgtcgcatc
      901 ggctgcaccg cggtatcacg gagccgattc gcggcgtggt cgacgtcgca cgcaacgtgc
      961 tcgacaagcg cgattactcc gcgcgggcgc cgaaagggca cgacgacgag cttggcgtgc
     1021 tcgtcgacgc gttcaacaac atgctggacg aagttggcga gcgtaccgcg gtgctggagc
     1081 gcgcgagaga ggagctgcgg acgctcaatg ccgagctcga gcaacgcgtc gccgcgcgaa
     1141 cggcgcagct cgagacggcg aacaaggagc tcgaaggctt cagctattcg gtgtcgcacg
     1201 atttgcgcgc gccgctccgg gccgttggag gcttcgcgga gctcctgcgc gacgatcacg
     1261 cggctcagct cgacgcggag ggcttacgca agctcgacgt catacgcgac gaagccgctc
     1321 gaatgggaac gttgatcgac gatctgctgg cgttctcgcg gctgggccgc aaatcgttgc
     1381 agcccgccga gctcgacatg gccgatctcg tgagcaacgt ggtcgaccgg ctgcgcgcgg
     1441 actcaggcgg cgagcgcgtg gcctttcgca tgggccgctt accgcgcgcc tggggtgacc
     1501 gcgctctgct cgagcaagtg tgggtgaatc tgctgtcgaa cgcggtgaaa ttctcgtcga
     1561 agaaggacgc tcccgtcgtc gaggtcggcg ccatttccgc ggaacgcgag cacgtgtttt
     1621 tcgtccgcga caatggcgcc ggtttcgatc cgcgctatgc ctcgaagctg ttcggagtgt
     1681 ttcagcggtt gcacgacggc gcggagttcc cgggcaccgg cgtcgggctt gcgctcgtgc
     1741 aacgcatcgt cgtgcgccac ggcgggcgcg tctgggccga cagcaagcca ggcgagggcg
     1801 cgacgttcca tttcacgctg ccgaaggaga attctcatgc aggagtttga gcacgtcgag
     1861 atcctgctcg tggaggacaa tccgcacgac gcggagctcg cgatacgcgg actcgagaag
     1921 cacaaggtcg cgaacaaggt cacgtgggtc aagaacggcg aagaagcgct cgacttcgtg
     1981 ttccgccggg gcatgttcga aggacgtacc gatgccggcc cgcgtctgat tctgctcgat
     2041 ctgaagatgc cgaaggtcga cggtatcgaa gtgacgcgcg ccgtcaaagc cgatccgcga
     2101 actcggcata taccgatcgt gatcatgacg tcgtcgcaag aggagaagga cgtcgcgaac
     2161 ggctacagct gcggcgcaaa cagctacatc gtgaagccgc tcgacttcaa gtcgctggtc
     2221 gacgtcgccg cgcaggtcgg cttctactgg ctcgcgatca atcgcgcgca accgtaaagc
     2281 tacgtcgaac gcgagcgccg gctcgccgcc ttcgcccgct tttcggcgcg cggcgcggcg
     2341 tattgcttga gcttgccgaa ggtctcgccg gtcaaccacc gtccgagatc ggcggcggtc
     2401 acgggccggc tcacgaaata gccttgcacg gcgtcgcatc cgagcgccct cagaagctcg
     2461 agctgctcga gcgtctcgac gccctcggcg acggttttca tgcccaagga ggcagcgagg
     2521 cgaatcgtcg tctgcacgat gaggcggtga ttgcgattcg tgacgaggtc gctcacgaag
     2581 ctgcggtcga tcttcaggac gtcgatcggt aggcctgcga gcacccgcag cgacgaatga
     2641 ccggtgccga agtcgtcgat cgcaatcgag acacccgcag cgcgcaattc tgcgaggctc
     2701 acgctcgaat gacgcgggtc ggccatcaag gtgctctcgg tgacttcgag ctcgagccga
     2761 cgcgggttgc cgccgagctc ggcgatcgaa tcgagcagcg ctcgtgcgaa atcctcgcgg
     2821 ttcagctgga gcggggagac gttcgcagcg acgcggaaat ccgcggccgt tgcccactgg
     2881 gccgtctcgg tcatcgcttg gacgacaacc cagctgccga cttcatcgat gaggcgcagc
     2941 gactcgagca tcggcacgaa cacgttcggc ggcaccggct cgctgtcggg cgaccaacgc
     3001 aggagcgcct cgaccccgtc gacgccgccc gtcttcaggt tgagcttcgg ctggtagtgg
     3061 agcgtgaagc cgcgctcggc aaccgcgcgg cgcagctggt tcgtcagcag gaagcgttgc
     3121 gaggcggtct cgttcatgcg ggcgttgtag cgcaagcagc gggcgcccgt gtccttcgcc
     3181 tgctgcagtg ccaccttcgc ctgctgcagc agtgcctccg catcgtcgcc gtcgtcggga
     3241 aactccacga atccagcgcg ggcgtcaacc cgcaagtcct ggctttgcag cgagaacggc
     3301 gcttcgaagc agtcggcgat gcgtttggct agccctcctt cggcgtggac cggttcgatc
     3361 tccagcacgg agaacttgtc gtcgccgagg cgactcacgg cggtggagtg gccgaacgca
     3421 tgcgtcaaac gctcggcgac gagccgtagc agcgagtcgc ccacgtgctg cccgagattg
     3481 tcgttgacgg tgccgagttc gacgatgtcg atgacggcaa cggcgacgga cttgccggct
     3541 tttttcgcgg agtcgagcac agcttgagct cgcgtcacat acaaggctcg gttcggcaag
     3601 cccgtcaacg tgtcgtagta ctcgagatat tcggcgttct ctttgctgcg caggtactgc
     3661 agcgcgaagg taatgtttgc cgccacttgc ttcagcagcg c
//
LOCUS       DASSNN010000099         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1028884, whole genome shotgun sequence.
ACCESSION   DASSNN010000099 DASSNN010000000
VERSION     DASSNN010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028884"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VFL84_01810"
     CDS             complement(<1..1103)
                     /locus_tag="VFL84_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7607383.1"
                     /translation="MRPFEARNRHFDRLFNTPGLMWLGQNTNHFHPHPAVVQAMLDAI
                     RHEEYHAYAPPAGFEELRALILQDLGLRDHSVLVTDGAVEALYNVCHTLCGPGDEFVT
                     TDPGWKWPIDFARAAGARTIEIPIYKPEHGYKLTIAQLEAAVTDKTRLIYLVDPNNPL
                     GVCYSAAEIEAFTRIARGVGAYFMHDCTYAHFADDHTLAARYYPERAVTIYSFSKWLG
                     LAGLRIGAVVAQPDLVERLAAAPPNNLGCNVISQRAAIAGLRIRSEWFPEINRRQRRN
                     QAEIAKAVAKVPGMAIAVYPSQANFLVVECIDAGVAPEALCEAYRAENIMIRQGTYHT
                     PTFGHRFVKVSTTVPEEWVDAFCERLPDMVAKAR"
     gene            complement(1100..1903)
                     /locus_tag="VFL84_01815"
     CDS             complement(1100..1903)
                     /locus_tag="VFL84_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7607384.1"
                     /translation="MDLRLAGKSVLITGASKGIGLAAARAFAAEGCNLHLAARSADAL
                     AAARDEIVREHRVGVEVHPIDLGRTQNVVDLGRRCAGVDILVNNAGEIPSGPIESLTE
                     DDWRRGYDLKLFGYITLTRELYAAMKARGNGVIINDIGNSGENWDANYIAGSTANAAL
                     MAFTKALGGQSLDFGVRVVGVNPGPVETERMVKINKRRALDWYGDESRWTELREKYPG
                     GRPASAEEVADLIVFLASPRAGYITGSIVTIDGGIAARGSIIKERSART"
     gene            1980..>2290
                     /locus_tag="VFL84_01820"
     CDS             1980..>2290
                     /locus_tag="VFL84_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7607385.1"
                     /translation="MSNVQRARPAPPGSRRPNGSTLTDRIRQAVQRDIVAGKLRPGAK
                     LDEDSLAARHGASRTPVREALQHLASQGLIELRAHAGAFVATLSVVELAEMFETMAFF
                     EA"
BASE COUNT          374 a          760 c          754 g          402 t
ORIGIN      
        1 cgcgccttcg caaccatgtc gggcaggcgc tcacagaagg cgtcgaccca ttcctccggc
       61 accgtcgtgc ttaccttgac gaagcgatga ccgaacgtcg gcgtatggta tgtcccctgc
      121 cggatcatga tgttctcggc acgatacgct tcgcacagcg cctcgggtgc gacaccggcg
      181 tcgatgcatt cgacgaccag aaaattcgcc tgcgacggat atacggcgat cgccatgcct
      241 ggcaccttgg cgacggcctt cgcgatctcg gcctggttgc ggcgctgtcg gcgattgatc
      301 tcggggaacc actcgctgcg aatccgcagg cccgcgatcg ccgcgcgttg cgagatcacg
      361 ttgcagccaa gattgttggg cggcgcggcg gccagccgct cgaccaggtc cggctgggca
      421 acgacggcgc cgatgcgcaa gccggcgagg cccagccatt tggagaagct gtagatcgtc
      481 accgcgcgct cggggtagta ccgtgcggca agcgtgtggt cgtcggcaaa gtgcgcgtac
      541 gtgcagtcgt gcatgaaata cgcgccgacg ccgcgcgcga tgcgcgtgaa tgcctcgatc
      601 tccgcagcgc tgtagcagac gccgagcggg ttgttgggat cgacgaggta gatgagccgc
      661 gtcttgtcgg taaccgcggc ctcgagctgc gcgatcgtca gcttgtatcc gtgctccggc
      721 ttgtagatcg gaatctcgat cgtccgcgca ccggccgcgc gggcgaagtc gatcggccac
      781 ttccagcccg gatccgttgt gacgaactcg tcaccgggac cgcacagcgt gtgacagacg
      841 ttgtacagcg cctcgacggc accgtcggtg acgagcaccg agtggtcgcg cagcccgagg
      901 tcttggagga tgagcgcccg caactcctcg aaacccgccg gcggggcgta cgcgtgatat
      961 tcctcgtggc gaatcgcatc gagcatcgcc tgcacgacag cgggatgcgg gtgaaagtga
     1021 ttggtgttct gcccgagcca catgaggccc ggcgtgttga acagccggtc gaagtgccgg
     1081 ttgcgagcct cgaacggcct catgtgcgag cgctccgttc cttgatgatc gagccgcgag
     1141 cggcaatgcc gccgtcgatc gtgacgatcg acccggtgat gtagccggcg cgcggcgacg
     1201 cgagaaaaac gatcaggtcg gccacctcct cggcgcttgc cggccttccg cccgggtact
     1261 tttcacgcaa ctcggtccag cggctctcgt cgccgtacca gtcgagcgcg cgccgcttgt
     1321 tgatcttcac catgcgctcg gtttcgaccg gcccgggatt gacaccgacg acgcgaacgc
     1381 cgaagtcgag gctttgtcct ccgagcgcct tcgtgaaagc catcagcgcg gcgttggcgg
     1441 tgctgcccgc gatgtagttg gcgtcccagt tctcgccgga attgccgatg tcgttgatga
     1501 tgacgccgtt gccgcgcgcc ttcatcgccg catacagctc gcgcgtcagc gttatgtagc
     1561 cgaacaattt caggtcgtag ccgcggcgcc aatcgtcctc ggtcagcgac tcgatcggcc
     1621 cggacggaat ttcgccggcg ttgttgacga ggatgtcgac cccagcgcat cggcgcccga
     1681 ggtcaacgac gttctgcgtg cggccgaggt caatcggatg aacctcgacc ccgacgcgat
     1741 gctcgcggac gatctcgtcg cgcgcggccg cgagcgcgtc ggccgaccgc gcggccagat
     1801 gaaggttgca gccttcggcc gcgaaagcgc gcgccgccgc gaggccgatg cccttggaag
     1861 cgccggtgat cagcaccgac ttgccggcga gccgcagatc cattttcgac tcctgtatac
     1921 gatccggatt gtctcgcata caatttcccg gtgtcaaacg atgccgcgcc ccaagagcca
     1981 tgagcaacgt tcagcgcgcc cgccccgctc cgcccggcag tcgcaggccg aacggcagca
     2041 cgctcaccga tcgcatccgc caggcggtcc agcgcgacat cgttgccggc aaactgcggc
     2101 ccggtgcaaa gctcgacgag gattcgctgg cggcgcgtca cggggcctcg cgcacgccgg
     2161 tccgcgaagc gctgcagcat ctcgcgtcgc aggggctgat cgagttgcgt gcgcacgcgg
     2221 gcgcgttcgt cgcgacgctc tccgtcgtcg agctcgccga gatgttcgag acgatggcct
     2281 tcttcgaagc
//
LOCUS       DASSNN010000100         2410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_20559, whole genome shotgun sequence.
ACCESSION   DASSNN010000100 DASSNN010000000
VERSION     DASSNN010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20559"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..>2410
                     /locus_tag="VFL84_01825"
     CDS             23..>2410
                     /locus_tag="VFL84_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607386.1"
                     /translation="MSRWRGVDLVDGDNLIAATVVDEAGREVAALERSVRYGSGAVRA
                     ELVAEESHLTADGRTQPVIALRVFDAAGEPARPGTLGAYRVDPPYRTWWEVETLHDNP
                     LIVESRREPTFAVEEDGLVRILLEPTTESGTALVRLRFNERQEQEIRAWLEPEQRDWI
                     LVGIAETTTAYTNLEAALEPPDIEDGYVGDGRLAFFAKGRIKGSSLLTIAFDSERDRP
                     LAEDRLFGAIEPDRYYTLYGDAVEQRFEAPTTRKLYLKIERRQFAALFGDFETGLNVT
                     ELSRYSRTLTGLKADFAGDRFAVNAFAAENRERYGRDELVGDGTSGPYRLSRTPLVAN
                     SDRLRIEVRDRVRSEVVVESRVLTRFIDYSIDCYTGVITFKQPVSSRDSEFNPVFIVA
                     EYETLSAEAGGTTAGARASARLSDTVELGATLVDEGSSAGAARLAGTDLTFRPTGSLE
                     VRAELAQTSSETMNRASAYLAEVEHVTERVDLSAYVREQKTGFGVGQQLHTETGTRKV
                     GIDARGTLTDLWTARGETFRQTNLATGADRSLASAEARRETDDATASVGLKRVVDDLP
                     QSGVQESQLLSLGGSLDVLHDRVTLRALTEQAVASRAASLDFPERTALGLDYHLSNAT
                     TLFAEVEDAEGELIDSTMTRVGVRSTPWSGMQLQSSVNHEFGEYGPRVFADVGLTQAF
                     KVGEAWAMDFGVDRSDTLTGAGAERFNANVPLVGGSMGGDYTATYLGAQYRADVWTIT
                     TRLERRDADTDQRRSFTSGFFREPLSGRALSVTTRWLDNETATGEGRIVDTRVSYA"
BASE COUNT          384 a          865 c          841 g          320 t
ORIGIN      
        1 gaacgccgcg aagacggagt cggtgagccg ctggcgcggc gtcgacctcg tcgacggcga
       61 caacctgatt gccgcaaccg tcgtcgacga ggccggccgc gaggtcgccg cgctcgagcg
      121 cagcgtgcgc tacggcagcg gcgccgtgcg cgcggagctc gtcgccgagg aatcgcacct
      181 gacggccgac ggccgcacgc agcccgtgat cgcgctgcgc gtcttcgacg cggccggcga
      241 gcccgcgcgg ccgggcacgc tcggcgccta tcgcgtcgac ccgccgtacc gcacctggtg
      301 ggaagtcgag acgctgcacg acaatccgct gatcgtcgaa agccgccgcg agccgacgtt
      361 cgccgtcgag gaagacggcc tcgtgcgcat cctgctcgag ccgacgacgg aatcgggcac
      421 ggccctcgtg cgcctgcgct tcaacgagcg ccaagagcaa gagattcgcg cctggctcga
      481 gcccgagcag cgcgactgga ttctggtcgg catcgcggag acgacgaccg cgtacacgaa
      541 cctcgaggcg gcgctcgagc cgcccgacat cgaggacggc tacgtcggcg acggacggct
      601 cgcgttcttc gcgaaaggcc gcatcaaggg ctcgtcgctg ctgacgatcg cgttcgattc
      661 cgagcgcgac cgcccgctcg ccgaggaccg cctgttcggc gcgatcgagc ccgaccgcta
      721 ctacacgctc tacggcgacg ccgtcgagca acggttcgaa gcgccgacga cgcgcaagct
      781 gtatctgaag atcgagcggc gccagttcgc ggcgctgttc ggcgacttcg agacgggcct
      841 caacgtaacg gagctgagcc gctacagccg cacgctgacg ggcctcaagg ccgacttcgc
      901 gggcgaccgc ttcgccgtca acgcgttcgc ggcggagaac cgcgagcgct acggccgcga
      961 cgagctcgtc ggcgacggca cgtcgggtcc gtatcggctt tcgcgcacgc cgctcgtcgc
     1021 gaacagcgac cggctgcgca tcgaggtgcg cgaccgcgtg cgcagcgagg tcgtcgtcga
     1081 gtcgcgcgtg ctgacgcgct tcatcgacta cagcatcgat tgctacacgg gcgtcatcac
     1141 gttcaagcag cccgtctcga gccgcgactc ggagttcaac cccgtcttca tcgtcgccga
     1201 gtacgagacg ctctccgccg aggccggcgg cacgacggcc ggcgcgcgcg cctcggcgcg
     1261 gctcagcgac acggtcgagc tcggcgcgac gctcgtcgac gaaggctcga gcgcaggcgc
     1321 tgctcggctc gcgggcaccg acctcacgtt ccggccgacg ggctcgctcg aggtgcgcgc
     1381 cgagctcgcg caaacgtcgt cagagaccat gaatcgcgcc tccgcgtatc tcgcggaggt
     1441 cgagcacgtg accgagcgcg tcgacctctc ggcctacgtg cgcgagcaga aaacgggctt
     1501 cggtgtcggc cagcagctcc acacggagac cggcacgcgc aaggtcggca tcgacgcgcg
     1561 cggcacgctg acggatctct ggactgcgcg cggcgagacg ttccggcaaa cgaatctcgc
     1621 gaccggcgcc gaccggtcgc tcgcatcggc cgaggcgcgc cgcgaaaccg acgacgcgac
     1681 ggccagcgtc gggttgaagc gcgtcgtcga cgacttgccg cagagcggcg tgcaggaatc
     1741 gcagctcctg agcttgggcg gcagcctcga cgtgctgcac gaccgcgtta cgctgcgcgc
     1801 gctgacggag caagcggtcg cgtcgcgggc cgcgagcctc gatttccccg agcgcacggc
     1861 gctcggcctc gactaccacc tctctaacgc gacgacgctg ttcgccgagg tcgaggacgc
     1921 cgagggcgag ctcatcgact cgacgatgac gcgcgtcggc gtgcgcagca cgccgtggtc
     1981 gggcatgcag ctgcagtcga gcgtgaacca cgaattcggc gagtacgggc cgcgcgtgtt
     2041 cgcggacgtc gggctcacgc aagccttcaa agtcggcgag gcctgggcga tggacttcgg
     2101 cgtcgaccgc agcgacacgc tgaccggcgc cggcgccgag cggttcaacg cgaacgtgcc
     2161 gctcgtcggc ggctcgatgg gcggcgacta caccgcgacc tacctcggcg cgcagtatcg
     2221 cgccgacgtc tggacgatca cgacgcggct cgagcgccgc gacgccgaca cggaccagcg
     2281 ccgctccttc acgagcggct tcttccgcga gccgttatcg ggccgcgcgc tctcggtcac
     2341 gacgcgctgg ctcgacaacg agaccgcgac cggcgaaggc cgcatcgtcg acacacgggt
     2401 ctcgtacgcg
//
LOCUS       DASSNN010000101         4029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1155931, whole genome shotgun sequence.
ACCESSION   DASSNN010000101 DASSNN010000000
VERSION     DASSNN010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155931"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFL84_01830"
     CDS             complement(<1..565)
                     /locus_tag="VFL84_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7607387.1"
                     /translation="MTTTAIELRAVTKTFGSLTAVDRLDLAIPRGALYGFIGPNGAGK
                     TTAIRMIMSILLPDSGELRVLGARSALDAKDRIGYLPEERGLYRKMKVAAYLTFIARL
                     KGVAERGLKERVHSAIERIGLKGVENKRCEELSKGMSQKIQILAAIIHEPDLLILDEP
                     FSGLDPVSTRLLKDLILAQHARGATVLF"
     gene            complement(570..1706)
                     /locus_tag="VFL84_01835"
     CDS             complement(570..1706)
                     /locus_tag="VFL84_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607388.1"
                     /translation="MLRSSSGCFVKTYSVRLLALLLAVAPGIAHAQTAWLLVPRAAQS
                     AVVRAEGQTVRDIVTFRGSAASYGESRSAVVVVGAEWVYVIDKESATVTYFLPATQPG
                     YVGYPVEDLSGPLRNTLVTDSHALFSIYRFPGQAPPNELGGQYDIVRMSLADGSLKSI
                     PLPEDCLHPQLAELAGVPVLYAWGSDLAFKLDAANGGVVRVQGNDDVADLRTKEARDR
                     RSLRGAPPAFIRYAVVAGAGSFRLSRFGELDHVLDERLATVSGPGGSIWLGPAHEVLA
                     LRSGLTDGKPAIGVVRQREAVRLQEGGTLSILDASSLDIVWTSDVARGAIPESFYAAN
                     DGSFFYIDRVSGDVVRLSREGTSTSWHLPPGQSRSARILSIERD"
     gene            complement(1712..2083)
                     /locus_tag="VFL84_01840"
     CDS             complement(1712..2083)
                     /locus_tag="VFL84_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011769898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HET7607389.1"
                     /translation="MDRSINVFGEALQGCNDKPKTGFFRDGCCNTSDQDVGSHTVCVE
                     VTKEFLEFSRFRGNDLSTPHPEFNFPGLKPGDRWCLCAARWLEAHEHGMAPRVLLKGT
                     HSRALEIVPLALLKKFAVDLN"
     gene            complement(2245..3099)
                     /locus_tag="VFL84_01845"
     CDS             complement(2245..3099)
                     /locus_tag="VFL84_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET7607390.1"
                     /translation="MFTGFKDKVGSCVVGALALVFAASTEAQQSAPRIFNTAKLKLQR
                     GEQIVGGTVTSPDPDIYCAMANSGFDFLWIEMQHSPLTYQEVARMIWACRGAPAMPFI
                     RVPDSTPGDIQKAMDIGGLGIIIPLVDSVEEIRSAVDYTMYPPRGKRSLGNGQYGALY
                     GNDYRQIANDNVVIVAMIESPAGVAIADEIASVPGVDVVFAASTDLGSFSGFRQGQPD
                     YEAMVTTIHDATLEAKRRLGGPLAWRNRKDFTFFQAPTESSFIRLGVEAALKAAPSGQ
                     APTEGAER"
     gene            complement(3172..3534)
                     /locus_tag="VFL84_01850"
     CDS             complement(3172..3534)
                     /locus_tag="VFL84_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607391.1"
                     /translation="MRPTLHFAIIACAFGVAPKLSGQPIRPTVEHNDMRLVVSYVSTG
                     ELLNLQAKYGGARPDRHYVRQSHRRGFSLLRRHRETGALTCEIYLSNEQRPREVDDEA
                     TLSLGHELLHCMLGDYHR"
     gene            3631..>4029
                     /locus_tag="VFL84_01855"
     CDS             3631..>4029
                     /locus_tag="VFL84_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET7607392.1"
                     /translation="MDVTYRFNGFRIDPIRRILFGPDERSIPLKPRVFDTLLYLVEHR
                     GQLLGKHVLLDAIWPHVVVEENNLNQAISTLRRVFGETRDEHRFIVTEPGRGYRFVAR
                     VETVPAETDAPIATQELVDWQIAEGIHFLVP"
BASE COUNT          710 a         1305 c         1290 g          724 t
ORIGIN      
        1 agaacagcac cgtcgcgccg cgggcgtgct gcgccaggat caggtccttc aagagacgcg
       61 tgctgacggg gtcgaggccg ctgaacggct cgtcgaggat cagcaagtcc ggctcatgga
      121 tgatcgcggc gagaatctga atcttctgcg acatgccctt cgagagctcc tcgcagcgct
      181 tgttctcgac gcctttgagc ccgatgcgct cgatggccga gtgcacgcgc tccttcaagc
      241 cgcgctccgc gacgcctttc aagcgcgcga tgaacgtgag gtacgcggcg accttcatct
      301 tgcgatagag gccgcgctcc tcgggcaggt agccgatgcg gtccttcgcg tcgagcgccg
      361 aacgcgcgcc gagcacgcgc agctcgcccg agtccggcag cagaatcgac atgatcatgc
      421 gaatcgccgt cgtcttgccg gcgccgttcg ggccgatgaa tccgtacagg gcgccgcgtg
      481 ggatcgcgag atccagccgg tcgacggcgg tgagactgcc aaaggtcttc gtgacagcgc
      541 gaagctcgat cgccgtggtc gtcacagggt cagtccctct ctatcgagag aattcgcgcg
      601 gaacggcttt gccccggtgg taaatgccaa ctcgtcgacg tgccttcccg cgacaggcga
      661 acgacgtcgc cgctgaccct gtcgatgtag aaaaaggagc cgtcgttcgc cgcatagaag
      721 ctttccggga tcgcgcctcg agccacgtcg ctcgtccata cgatgtcgag cgaggaggca
      781 tccaagatgg acaacgtgcc accctcttgt agtcttacag cctcccgttg tcttacaacg
      841 ccgattgccg gcttgccgtc ggtcagcccc gatcgcaatg ccagcacctc gtgcgccggg
      901 cccagccata tcgagccccc gggaccgctg actgttgcga gccgctcgtc gagcacgtgg
      961 tccaattcgc cgaacctcga cagcctgaac gaaccggctc cggccacgac cgcatagcgg
     1021 atgaacgcag gaggcgcgcc gcgcaggctt ctgcgatcgc gcgcctcctt agtcctcagg
     1081 tccgccacat cgtcgttccc ctgaacgcgc acgacgcctc cgtttgccgc gtcgagcttg
     1141 aaagccagat cgctgcccca cgcatacagg acgggcacac cagccagctc cgccaactgc
     1201 ggatggaggc aatcctcggg caacggaatg gacttcagcg agccgtccgc aagcgacatt
     1261 cgaacgatgt cgtactgccc acccaattca ttcggcggag cttgtccggg aaaacgatag
     1321 atcgaaaaga gcgcgtgact gtcggtcacc aacgtgtttc tgagcgggcc cgagagatct
     1381 tcgacgggat agccgacgta tcccggctgt gtcgcgggca gaaaataggt gaccgtcgcc
     1441 gactccttgt cgatcacgta gacccattcg gcgccgacta cgactacagc gctgcgcgac
     1501 tcgccgtagc tcgccgcgga gccgcgaaag gtcacgatat cgcgaacggt ctgaccctcc
     1561 gccctgacga ccgcggactg agccgcgcgc ggcacgagca gccacgccgt ttgcgcgtgc
     1621 gcgatgccgg gcgcgactgc gaggagcaga gccagcaagc ggacgctgta cgttttcaca
     1681 aagcagccac tcgacgagcg cagcaagcgc tctagttgag atcgacggcg aacttcttca
     1741 gcagcgcgag cggcacgatc tcgagcgcgc gcgagtgcgt gcccttcaag agcacacgcg
     1801 gcgccatgcc gtgctcgtgc gcttcgagcc aacgggccgc gcacaagcac cagcggtcgc
     1861 cgggcttcaa gcccggaaag ttgaactcgg ggtgcggcgt cgacaggtcg ttgccgcgga
     1921 agcgcgagaa ctcgaggaac tctttggtga cctcgacgca aaccgtatgg gagccgacgt
     1981 cctgatcgct cgtattgcaa cagccgtcgc gaaagaagcc ggtcttgggc ttgtcgttgc
     2041 agccctgcag cgcttcgccg aatacgttga tggagcgatc cacggaagac cttcgagttg
     2101 ccgctgtttc cgtgagcgta gcgcggcagc gctcgcgcgg ctagccgaag ctagacccgg
     2161 cggcttcgat cgcatcgcgg gcttcttcgc agctgggtgt gtgtctcatg caccccgctc
     2221 cgcaatcgtc gccgattgcg acgtttagcg ttcggcgcct tccgtcggcg cttggcccga
     2281 cggcgcggct ttcagcgccg cctcgacgcc gagccgaatg aagctgctct cggtcggcgc
     2341 ctggaagaac gtgaagtcct tgcgattgcg ccacgcgagc ggcccgccga gccggcgctt
     2401 cgcctcgagc gtcgcgtcgt ggatggtcgt gaccatcgcc tcgtagtcgg gctgcccttg
     2461 gcggaagccc gagaagctgc ccaggtccgt gctcgccgcg aacactacgt cgacacccgg
     2521 cacgctcgcg atctcgtcgg cgatcgcgac gccggccggc gactcgatca tcgcgacgat
     2581 cacgacgttg tcgttggcga tctgccgata gtcgttgccg tacagcgcgc cgtactggcc
     2641 gttgccgagg ctgcgcttgc cgcgcggcgg atacatcgtg tagtcgacgg cgctgcggat
     2701 ctcctcgacg ctgtcgacga gcggaatgat gatgccgagc ccgccgatgt ccatcgcctt
     2761 ctggatgtcg cccggcgtcg agtccggcac gcgaatgaac ggcatcgccg gcgcgccgcg
     2821 gcacgcccag atcatgcgcg cgacctcttg gtacgtgagc gggctgtgct gcatctcgat
     2881 ccagaggaag tcgaagcccg agttcgccat cgcgcagtag atgtcgggat cgggcgaggt
     2941 cacggtgccg ccgacgattt gctcgccgcg ctggagcttc aatttcgcgg tgttgaagat
     3001 gcgcggcgcg ctctgttggg cctccgtgct ggccgcgaat accagcgcca gcgcgccgac
     3061 gacgcaagaa ccgaccttgt ccttgaaacc ggtgaacaac tgagggactc ctcgcgccga
     3121 tgctgcggcg agcatagggc tggcgcgtgc cgagcgtcaa cgccgagcgg ctcagcgatg
     3181 gtagtcaccg agcatgcagt gcagcagctc atggccgagg ctcaatgtcg cctcgtcgtc
     3241 gacctcgcgt ggacgctgct cgttggacag atagatctcg cacgtcaggg cgcccgtctc
     3301 gcgatgcctt ctcaagagcg agaagccgcg acggtgactc tggcggacgt agtggcgatc
     3361 gggccgagcg ccgccgtact tggcttggag gttcaagagc tcgccggtgg atacgtaact
     3421 gactactagc cgcatgtcgt tgtgctcgac ggtcggcctg atgggctgtc cactcagctt
     3481 cggcgcgacg ccgaacgcgc acgcgatgat cgcaaagtgg agtgtcggcc gcatggggcg
     3541 ctagaatcgg ccgcgcccgc ttgaacgctc tttggtggtc gtttaatcgt tctgaatcgt
     3601 tctgaatcgt actgatgagg agtacctttc gtggacgtga cgtatcgatt caatggcttt
     3661 cgtatcgatc cgatacggcg aatcctgttc gggccggacg aacggtcgat tccgctgaag
     3721 ccgcgcgtct tcgatacgtt gctctatctg gtcgagcacc ggggccagct tctcgggaaa
     3781 cacgtcctgc tcgatgcaat ttggccgcat gtcgtggtcg aggaaaacaa cctcaaccag
     3841 gcgatatcga cgctgcgccg cgtattcggt gaaacccgcg acgagcatcg cttcatagtg
     3901 accgagcccg gccgtggtta ccgcttcgtc gcgcgcgtgg aaaccgtgcc cgccgaaacg
     3961 gacgcaccga tcgcaaccca agagctggtg gactggcaga tcgcggaggg aattcatttc
     4021 ctcgtgccc
//
LOCUS       DASSNN010000102         3790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1155955, whole genome shotgun sequence.
ACCESSION   DASSNN010000102 DASSNN010000000
VERSION     DASSNN010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155955"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VFL84_01860"
     CDS             <1..1110
                     /locus_tag="VFL84_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET7607393.1"
                     /translation="EEGKYYLWTREQFDAALSEPEARLAKRAFGLEKPSNFEDRAWHL
                     WLPETSTDGNEGKQNDEGKQNDESKQSDEGKQNDETAVLARARAKLLEARERRVAPGR
                     DEKLLVSWNGLMIRGMAKAARRLGRPDLAASAARAVDFIRANLWRDGRLCATYKDGRA
                     RLAGYLDDYAFLADGLLELLEQRWRTEDLELAIALTDVVLAHFEDASGGFFFTADDHE
                     KLIHRPKPWLDEAVPSGNAVMLRVLLKLGALLGERRYEESAERGLKAALPTTGQYPDA
                     HAALMLALARYLEPPEIIVVRAEAGELPSWRAIVDAGYAPRRSAYCIPNDAPRLPGLL
                     ADRAPRGAAVAYVCLGTECRAPITELEQLRSALSR"
     gene            complement(1283..>3790)
                     /locus_tag="VFL84_01865"
     CDS             complement(1283..>3790)
                     /locus_tag="VFL84_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7607394.1"
                     /translation="ATVGRDIRVVGNDAGEQLSFLAGTIARLDRQAPNYGRGNYNDFN
                     TFYLQAASGTSGGSSGSPVIDIQGQVVALNAGANTQAQTSFFLPLDRVRVALHLIQQG
                     LPVPRGTVETEFVHQPYAELRRLGLTRQTEAAVREADPTNTGMLVVKQVIPGSPASGK
                     LQPGDILLRIDGKPIYEFVPLEATLDENVGKTVPVEIERGGQELTQNIGIHNLYGITP
                     DEYVEYGDGVLHELSYQQARHYYRPAKGIYVADPGYVFGSASIPRASIITEIDGQQVV
                     TLDDLQRVLESLPEGAQVSVRFIEFDDPRIVHEAIFTNNRSWFTAARCKRDDSTGLWP
                     CKPFPPAPAAKPLVPQTTTFPPESSPVLERIAPSLVFVNFSMPYTISGVSDRYYYGTG
                     LVVDAQRGWIVVDRNTVPVAMGDVRLTFAGSIEIPGHVEYVNPLHNLAVVSYDPKLLG
                     DTPVRSAEFSDRKLSAGNQVDVVGFSPDDDLRSQSTQVASVSEADFPVSRTLRFRDSN
                     LETAALVNGPADFNGAILDSDGRVEGMWASFAVQAGRDVQQINMGLPSYMITDMLEHM
                     RKDEPLYSLDVEWRLLPLATARKLSLPASWGQRYEAHNPERREVLAVASTVAGSPAAN
                     FFRSGDILLSVDGKLANTFEEVERLSQAPDVNVTIERDGQEIERHVPTVALSGRGVDR
                     IVLWAGALVQSPHRALAAQRGVKPTGVYVSYFNFGSPASRAGLYAGRRIVAIDGRPTP
                     DLDSFIDVVKSLGRPDAVRLKTVNWNDVPEVITLEPDPLYWPSYALERVDYDWRRIPL
                     DASSANGAIQSGTPLPNPISGEGRNPTLKPQTAAPAG"
BASE COUNT          637 a         1239 c         1297 g          617 t
ORIGIN      
        1 gaggaaggca agtattactt gtggacccgg gagcaattcg atgcggccct cagcgagccc
       61 gaggcccggc ttgcgaaacg ggccttcggg ctcgaaaaac cgtcgaactt cgaagaccgg
      121 gcttggcatt tgtggctccc cgagacctcg accgacggga acgagggtaa gcagaacgac
      181 gagggtaagc agaacgacga gagcaagcag agcgacgagg gtaagcagaa cgacgagacc
      241 gcagtgctcg cccgtgcgcg cgccaagctg ctcgaggccc gcgagcggcg tgtcgcgccc
      301 ggccgcgacg aaaagctgct cgtctcgtgg aacggactca tgatccgcgg catggccaag
      361 gccgcgcggc ggctcggccg gccggacctc gcagcttccg cggcgcgcgc cgtcgacttc
      421 attcgcgcga acctttggcg cgacggccgg ctgtgcgcca cctataaaga cggacgcgca
      481 cggctcgcgg gctatctcga cgattacgca tttcttgccg acggtctact cgagctgctc
      541 gagcagcgct ggcgcacgga agatctcgag ctcgcgatcg cgttgaccga cgtcgtgctc
      601 gcgcatttcg aagacgcgag cggcggcttc ttcttcacgg ccgacgacca cgagaagctc
      661 attcaccggc cgaagccctg gctcgacgaa gccgtgcctt ccggcaacgc cgtgatgctg
      721 cgcgtgctgc tcaagctcgg cgctctgctc ggcgagcggc gctacgagga gtcggccgag
      781 cgagggctca aagcggctct gccgaccacc gggcaatacc cggacgcgca cgcggcgctg
      841 atgctcgcgc tcgcgcgcta cctcgagccg cccgagatca tcgtggtacg cgccgaggcc
      901 ggcgagctgc cgagttggcg cgcaatcgtc gacgcgggct acgcgccgcg ccggtcggcc
      961 tattgcattc cgaacgacgc gccgcgcctg ccgggattgc tcgccgaccg cgcaccgcgc
     1021 ggcgccgccg tcgcctacgt ctgtctcggc accgaatgcc gagctcccat taccgagctc
     1081 gagcaactgc ggagcgcgct aagccgatag tgagccccag gcttggctgc gctgcagggc
     1141 aggccgtagc gcgggagtcc gcgggaggac cgcaaccgcg aggcgaatta gccgcacctc
     1201 gcagccgcga attagccgca ccccgcaacc gcgagttagc cgcaccccgg gtaggccgca
     1261 tcgctcccta cccggccgct tgctagcccg ccggcgccgc ggtctggggc ttcagcgtcg
     1321 gattgcgtcc ctccccggaa atcggattcg gcagcggagt gccgctctgg atcgcgccgt
     1381 tcgcggagga cgcgtcgagc gggatgcgcc gccaatcgta gtccacgcgt tcgagcgcat
     1441 agctcggcca gtaaagggga tccggctcca aggtgatgac ctcgggcacg tcgttccaat
     1501 tgaccgtctt gagccgcacg gcgtcgggcc gccccaagct tttcacgacg tcgatgaagc
     1561 tgtcgaggtc gggggtcggc ctgccgtcga tcgcgacgat gcgccggccg gcgtataggc
     1621 cggcacggct cgccggggag ccgaagttga agtacgagac gtagacgcca gtcggtttca
     1681 cgcccctttg tgccgccagc gcgcgatgcg gcgattgcac gagcgcgccg gcccacagca
     1741 cgatgcgatc gacgcctcgc ccgctcaacg cgacggtcgg gacgtgcctc tcgatctcct
     1801 ggccgtcccg ctcgatcgtg acgtttacgt ccggcgcttg cgagagccgc tcgacttcct
     1861 cgaaggtgtt ggcgagcttg ccgtcgacgc ttagcaggat gtcgcccgag cggaagaaat
     1921 tcgccgcggg cgagcccgcg acggtgctcg ccacggccag cacttcgcgg cgctcggggt
     1981 tgtgcgcctc gtagcgttga ccccaggaag cgggcaggct cagcttgcgt gctgtcgcga
     2041 gcggcaggag acgccattcc acgtcgagcg aatacagcgg ctcatccttg cgcatgtgct
     2101 cgagcatgtc ggtgatcatg tagctcggaa ggcccatgtt gatctgctga acgtcgcgcc
     2161 cggcttgcac cgcgaagctc gcccacatgc cctcgacgcg tccgtcggaa tccaggatcg
     2221 cgccgttgaa atcggccggg ccgttgacga gcgccgcagt ttcgaggttc gagtcgcgaa
     2281 agcgaagcgt gcgcgagacg gggaaatccg cttcgctgac cgacgcgacc tgggtcgatt
     2341 gcgagcgcaa atcgtcgtcc ggcgagaagc ctacgacgtc gacctggttg ccggccgaaa
     2401 gcttgcggtc ggagaactcg gcgcttcgca cgggcgtgtc cccgaggagc ttgggatcgt
     2461 aagagaccac ggcgaggttg tgcagcggat tcacgtattc cacatgcccc gggatctcga
     2521 tcgagcctgc gaacgtgagc cgcacgtcgc ccatggcgac gggcacggtg ttgcggtcga
     2581 cgacgatcca gccgcgctgg gcgtcgacga cgaggcccgt gccgtagtag tagcgatcgg
     2641 agacgccgga gatcgtgtac ggcatgctga agttaacgaa tacgagcgag ggcgcgatgc
     2701 gctcgagcac cggcgagctc tcgggcggga aggtcgtggt ctgcggcacg agcggcttcg
     2761 ccgccggcgc cggcggaaaa ggcttgcacg gccaaagccc ggtcgagtcg tcccgcttgc
     2821 agcgcgcggc agtgaaccag ctgcggttat tcgtgaagat agcctcgtgc acgatgcgcg
     2881 gatcgtcgaa ctcgatgaaa cgcacgctga cctgcgcgcc ctcgggcagc gactcgagca
     2941 cccgttgcag atcgtccagc gtcacgactt gctgcccgtc gatctcggtg atgatgctcg
     3001 ctcgcgggat ggacgcggag ccgaacacat agcccggatc ggccacgtaa atacctttcg
     3061 cgggccggta atagtgccgt gcctgctgat acgagagctc gtgcagcacg ccgtcgccgt
     3121 attcgacgta ttcgtcgggc gttatgccgt agagattgtg gatgcctatg ttttgggtga
     3181 gctcctggcc gccgcgttct atctcgacgg gcacggtctt gccgacgttc tcgtcgagcg
     3241 tagcctcgag cggcacgaat tcgtagatcg gcttgccgtc gatacgcagg aggatgtcgc
     3301 cgggctggag cttgccggaa gccggcgaac ccggaatcac ttgcttcacg accagcatgc
     3361 cggtgttcgt cggatcggcc tcgcgcaccg cggcttccgt ctgccgcgta agtccgagcc
     3421 ggcgcagctc ggcatacggc tggtgcacga actcggtctc gaccgtgccg cgcggcacgg
     3481 gcagcccttg ttgaatgaga tgcaaggcga cgcgaacgcg gtcgagaggc aggaagaagc
     3541 tcgtctgagc ctgagtgtta gcgcccgcgt tcagcgcgac cacctgccct tgaatatcga
     3601 tgaccggaga acccgaggag ccgcccgagg tgccggacgc cgcttgcaga taaaaggtgt
     3661 tgaagtcgtt gtagttgccg cggccgtagt tcggcgcctg gcggtcgagc cgcgcgatcg
     3721 tgccggcgag aaacgacagc tgctcgccgg cgtcgttgcc caccacgcgg atgtcgcgcc
     3781 ccaccgtggc
//
LOCUS       DASSNN010000103         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1029525, whole genome shotgun sequence.
ACCESSION   DASSNN010000103 DASSNN010000000
VERSION     DASSNN010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029525"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="VFL84_01870"
     CDS             complement(<1..1392)
                     /locus_tag="VFL84_01870"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HET7607395.1"
                     /translation="MTQSSASRTAARYKLASVYAERRRRRSVGAVVSDPARDALLTPF
                     GQATLRDRYLLPGESFQDLFARVARAYADDTAHAQRLYDYMSKLWFMPATPVLSNGGT
                     ERGLPISCFLNAVEDSLDAIVGTWTENVWLASNGGGIGTYWGAVRSIGETVKGNGQTS
                     GIIPFVRVMDSLTLAISQGSLRRGSAAVYLDVHHPEIEEFVEIRKPSGDFNRKSLNLH
                     HGIAVTDEFMECVRDDRPFALRSPATGAPLKSVSARLLWQKILETRLQTGEPYLLFID
                     AANRDLAAHQRELGLRVRQSNLCSEILLPTGRDHLGRPRTAVCCLSSVNFEKWDEWSG
                     EPGFVEDLMRFLDNVLDDFIERAPDSMERASYSAMRERSVGLGAMGFHSFLQARGIAF
                     ESALAKSWNLKMFRRLRADADAASRVLAAERGPCPDAADCGVPERFSSKLAIAPTASI
                     SIICGGTSACIEPI"
     gene            complement(1564..>2124)
                     /locus_tag="VFL84_01875"
     CDS             complement(1564..>2124)
                     /locus_tag="VFL84_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607396.1"
                     /translation="WWSGPAIRDIEVLMTPHEVQFTAGVADNFVRKILIGQKHVMQVP
                     QWYGESVGFWDGDTLVIWTANVQGWTLTHSMFEFSNSLEVIEVVTPMKSGKGLVVEAT
                     FYDPEAFKQPLHIVTPWNYVTGIDDPQKRYTFVECRVQSTIVNGPDGRPTELTPNDPG
                     YIDYFGRPWAQNWEKHFEQGWAHPKE"
BASE COUNT          361 a          693 c          697 g          373 t
ORIGIN      
        1 gatcggctcg atgcaggcgc tcgtgccgcc gcagatgatg ctgatgctcg cggtcggcgc
       61 gatcgcgagc ttgctgctga aacgctcggg cacgccgcag tccgccgcat ccgggcacgg
      121 gccgcgctcg gccgcgagca cgcgcgaagc ggcgtcggcg tcggcgcgca ggcggcgaaa
      181 catcttgagg ttccagctct tcgcgagcgc gctctcgaag gcgatgccgc gcgcctgcag
      241 gaacgaatga aagcccatcg cgccgaggcc caccgaacgc tcgcgcatgg ccgagtagct
      301 cgcgcgctcc atgctgtccg gggcgcgctc gatgaagtcg tcgagcacgt tgtcgaggaa
      361 gcgcatgaga tcttcgacga agccgggctc gccgctccac tcgtcccact tctcgaaatt
      421 cacggaggac aggcaacaca cggccgtgcg cggcctgccg agatgatcgc ggccggtggg
      481 cagtaggatc tcgctgcata ggttcgactg ccgcaccctg aggccgagct cgcgctgatg
      541 ggcggcgaga tcgcggttcg cggcgtcgat gaagagcagg taaggctcgc ccgtctgcaa
      601 gcgcgtctcg agaatcttct gccacagcag cctcgccgag acgctcttga gcggtgcgcc
      661 ggtcgccggg ctgcgcaggg cgaacggccg gtcgtcgcgc acgcactcca tgaactcgtc
      721 cgtcaccgcg atgccgtggt gcagattcaa gctcttgcgg ttgaagtcgc ccgacggctt
      781 tctgatctcg acgaattcct cgatctcggg atggtgcacg tccaaataga ccgccgccga
      841 gcctctgcgc agagaaccct gagagatcgc gagcgtcagc gaatccatga cgcgcacgaa
      901 cgggatgatg cccgaggtct ggccgttgcc tttgaccgtc tcgccgatgg agcgcacggc
      961 gccccagtaa gttccgatgc cgccgccgtt cgacgcgagc cacacgttct ccgtccacgt
     1021 gccgacgatg gcgtcgagac tatcttccac cgcgttcaaa aagcacgaga tcggcaaccc
     1081 gcgctccgtg ccgccgttgc tgagcacggg tgtcgcgggc atgaaccaga gcttcgacat
     1141 gtagtcgtaa agccgctgcg catgcgccgt gtcgtcggca taggcgcgcg cgacgcgggc
     1201 gaagagatcc tggaaggact cgcccggcag caggtagcga tcgcgcagcg tcgcctgacc
     1261 aaaaggcgtc aggagcgcat cccgcgccgg gtccgagacg acggctccga cggaccggcg
     1321 gcgccggcgt tcggcgtaca cgctcgctag cttgtaacgc gcggccgttc tcgatgcgga
     1381 agactgagtc atgcgtcgcc tcgtgctttt ggttctggtg acggcgacga gcgatccgga
     1441 cgtctacgcg ccccgcgcgc tcatcgcagc ggcccgtcga gtctctaacg gctcgatcgt
     1501 cagcgagtca gggacaccag atattggggc gcagacgccg ttaatgtcaa tatatgggaa
     1561 tactcattcc ttcggatgcg cccagccctg ctcgaagtgt ttctcccagt tctgcgccca
     1621 cggtcttccg aagtagtcga tgtagccggg gtcgttcggc gtgagctccg tgggccggcc
     1681 atcgggaccg ttgacgatgg tgctctgcac tcggcactcg acgaaggtgt agcgcttctg
     1741 cgggtcgtcg atgcccgtga cgtaattcca cggcgtcacg atgtggagcg gctgcttgaa
     1801 cgcctcgggg tcgtaaaagg tggcttcgac cacgaggccc ttgccgcttt tcatgggcgt
     1861 gacgacttcg atgacctcga gcgagttgct gaactcgaac atcgagtgcg tgagggtcca
     1921 gccctgcacg ttggccgtcc agatcacgag cgtgtcgccg tcccagaagc ccacgctctc
     1981 gccataccac tgcggcacct gcatgacgtg cttctggccg atgagaatct tccgcacgaa
     2041 gttgtccgcg acgccggccg tgaactgcac ctcgtgcggc gtcatgagca cctcgatgtc
     2101 gcgaatggcc ggaccggacc acca
//
LOCUS       DASSNN010000104         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_263061, whole genome shotgun sequence.
ACCESSION   DASSNN010000104 DASSNN010000000
VERSION     DASSNN010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263061"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..958)
                     /locus_tag="VFL84_01880"
     CDS             complement(53..958)
                     /locus_tag="VFL84_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7607397.1"
                     /translation="MPAGSAIPQHLLEPETLAGLANLDLVARAVVNGFLTGMHRSPFF
                     GFSQEFAEYRAYAEGDDPRFVDWNVYARTDRTYIKRYIGETNTRLVILLDASASMGFG
                     SGKVSKLQYAKFLAATLAYLAARQHDAVGLIVFDDKIREHRRASTRGGRLSGVLHAID
                     RAQPGTGTDMKIPFERFHEVEHGRGLVAVISDFYCDPDALLKSVQPLAYQGQDVILFQ
                     LLDPQELKPVMKESALIEDMESGQTMEVSPIYMSSKYRERINEHVEALHKSALRAGAD
                     HRLVNITEPLDAVLRSYLLFRQRRG"
     gene            complement(964..2004)
                     /locus_tag="VFL84_01885"
     CDS             complement(964..2004)
                     /locus_tag="VFL84_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7607398.1"
                     /translation="MSDAANTLDTQETRALIENFHSASEQIRAEVRKIIVGQDDVVEH
                     LLMTLLVGGHCLITGMPGTAKTLLVRTLASALGLTFKRIQFTPDLMPTDVTGTDILEE
                     DVASGRRTWTFVPGPLFTNVLLADEINRTPPKTQSALLEAMQEFSVTVRGHHHKIERP
                     FFVLATQNPIELEGTYPLPEAQLDRFLFNVLLDYLSAEDEVEVVNRNTTKMELPEIKA
                     CTTPEQILKFQWLVRQVPVSTTVNRYAVDLVRATRPHDPNAPEMVKKYVQYGASVRAT
                     QFITLAAKSRALMQGRYHVTHEDVASLALPVMRHRVLTNYFAESDGVDTDKVLTDLVK
                     QLGADNRQARVG"
     gene            complement(2001..>2379)
                     /locus_tag="VFL84_01890"
     CDS             complement(2001..>2379)
                     /locus_tag="VFL84_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607399.1"
                     /translation="LEYRKRGGYDPDALIALARMLGDAGRTDDSIAVFKDVLMVAPLR
                     PEVHRDFGDRLAAANRNREALVEYQAFLNMNPQDLAEAHYKLAKTYVALDDKAKGREH
                     LLYALEIAPHYREAQQLLLEVVR"
BASE COUNT          398 a          733 c          795 g          453 t
ORIGIN      
        1 gttgccgccg ggacgcctca tcgcaaagat tgactcaccg caaagaggca tttcagccgc
       61 gcctctgtct gaaaaggaga taagagcgga gaacggcgtc gagcggctcg gtgatgttga
      121 cgaggcggtg gtcggcgccc gcgcgcaatg ccgatttgtg cagcgcctcg acgtgctcgt
      181 tgatgcgctc gcggtacttc gagctcatgt agatcggcga gacctccatc gtctgcccgc
      241 tctccatgtc ttcgatcagc gccgactcct tcatgaccgg cttcagctct tgcgggtcga
      301 gcagctggaa caggatcacg tcctgcccct gatacgcgag cggctgcacg ctcttcaaga
      361 gcgcgtcggg gtcgcaatag aaatccgaga tgacggcgac gaggccgcgg ccgtgctcga
      421 cctcgtggaa ccgctcgaac gggattttca tgtccgtgcc cgtgcccggc tgcgcgcgat
      481 cgatcgcgtg cagcacgccg gagagccggc cgccgcgtgt cgacgcgcgg cgatgctcgc
      541 ggatcttgtc gtcgaacacg atgaggccga ccgcgtcgtg ctgccgcgcg gcgaggtacg
      601 cgagcgtcgc ggccaggaac ttcgcgtact gaagcttgct gaccttgccg ctgccgaatc
      661 ccatcgacgc gctcgcgtcg agcaggatca cgagccgcgt attcgtctcg ccgatatagc
      721 gtttgatgta ggtgcggtcc gtgcgcgcgt aaacgttcca gtcgacgaag cgcgggtcgt
      781 cgccttcggc gtaggcgcgg tactcggcga attcctggct gaagccgaag aatggcgagc
      841 ggtgcatgcc cgtcaggaaa ccgttgacga cggcgcgcgc gacgagatcg aggttcgcga
      901 gccccgcgag cgtttccggc tcgagcagat gctgaggaat cgcgctccct gccggcatgg
      961 tatttagcct acgcgggctt gcctattgtc ggcgccgagc tgcttgacga ggtcggttag
     1021 aaccttgtcg gtatcgacgc cgtccgactc cgcgaagtag ttcgtcaaca cgcgatgccg
     1081 catgacgggc agcgcgagcg acgcgacgtc ctcgtgcgtg acgtggtagc ggccctgcat
     1141 cagcgcgcgc gactttgcgg cgagcgtgat gaactgcgtc gcgcgtacgc tcgcgccgta
     1201 ctgcacgtat ttcttgacca tctcgggcgc attcgggtcg tgcggccgag tcgcgcgcac
     1261 gaggtcgacg gcatagcgat tgacggtcgt cgagacgggc acctgccgca cgagccattg
     1321 gaacttcaag atctgctcgg gcgtggtgca cgccttgatc tcgggcagct ccatcttcgt
     1381 cgtgttgcga ttcacgacct cgacctcgtc ctcggcgctc aagtagtcga gcagcacgtt
     1441 gaacaggaag cggtcgagct gcgcttcggg cagcgggtac gtgccctcga gctcgatcgg
     1501 gttctgcgtc gcgagcacga agaacggccg ctcgatcttg tgatggtggc cgcgcacggt
     1561 gaccgagaac tcctgcatcg cctcgagcag cgccgactgc gtcttcggcg gcgtgcggtt
     1621 gatctcgtcg gcgagcagca cgttcgtgaa cagcggcccc ggcacgaacg tccaggtgcg
     1681 ccggcccgaa gcgacgtcct cctcgaggat gtcggtgccc gtgacgtcgg tcggcatcaa
     1741 gtccggcgtg aactggatgc gcttgaacgt gagcccgagc gcgctcgcaa gcgttcgcac
     1801 gaggagtgtt ttggcggtgc ccggcatgcc ggtgatcaaa cagtggccgc cgacgagcag
     1861 cgtcatcagc aaatgctcga cgacgtcgtc ttggccgacg atgatcttgc gaacttccgc
     1921 gcggatttgc tcggaggccg aatggaaatt ctcgatcagc gctcgcgtct cttgcgtgtc
     1981 gagagtgtta gcagcgtcgc tcaacgtacg acctccagta gcagctgttg ggcttcacga
     2041 taatgcgggg cgatttccag agcatacagt aaatgctcgc gacccttggc tttgtcgtcg
     2101 agcgccacgt aggtcttggc gagcttgtag tgggcttcgg caagatcctg cggattcatg
     2161 ttcaagaacg cctggtattc gacgagcgcc tcacgattgc gattggcggc cgccaagcgg
     2221 tcgccgaaat cccgatgcac ctcgggccgc agcggcgcga ccatgagcac gtccttgaac
     2281 acggcgatcg agtcgtccgt gcggccggcg tcgccgagca tgcgcgcgag cgcgatcaga
     2341 gcatcgggat cgtagccgcc gcgcttgcgg tattcgagc
//
LOCUS       DASSNN010000105         5821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1775667, whole genome shotgun sequence.
ACCESSION   DASSNN010000105 DASSNN010000000
VERSION     DASSNN010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775667"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /gene="truA"
                     /locus_tag="VFL84_01895"
     CDS             <1..696
                     /gene="truA"
                     /locus_tag="VFL84_01895"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019624169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET7607400.1"
                     /translation="LAAAVSFVAGAPVIVHGSGRTDAGVHALHQVAHFDTAVARTPRQ
                     WLLGINSNLPADVAIRWVREVPDGFDARRSALSRRYRYTVLSQPARPALLRRRAWWLR
                     QELDCGAMTAAASAWLGEHDFSAFRGAGCQAKSPMRRLTAVAIARRRRGEGTLVTFEF
                     TANAFLQHMVRNLVGTLVAVGGGELTPQQAARILELRDRREAGVTAPPEGLALVEVLY
                     PAELALPRYEDEP"
     gene            754..1614
                     /gene="accD"
                     /locus_tag="VFL84_01900"
     CDS             754..1614
                     /gene="accD"
                     /locus_tag="VFL84_01900"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HET7607401.1"
                     /translation="MSWFDKLMPSRIRTEKRKHSVPEGLWVKCQGCSAQLYRAELNRN
                     LHVCPKCGHHLRIGARERLEMFLDAESGYELAANLEPLDPLRFKDTKRYRERLNQVQR
                     DTDERDALVVMAGTLKSMPVVACAFEFRFISGSMGSVVGERFVRAVAYCIQHQRPLVC
                     FSASGGARMQEGLYSLLQMAKTSSALAQLAAAKLPYVSVMTDPTTGGVSASLALLGDI
                     NIAEPGALICFAGPRVIEQTTGETLPEGFQRAEFLLEKGAIDMILDRRHSRDQIAALL
                     AKLSSRPALS"
     gene            1634..2962
                     /locus_tag="VFL84_01905"
     CDS             1634..2962
                     /locus_tag="VFL84_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET7607402.1"
                     /translation="MTTPPRGLTLDHWLAWFETVHPKRIDLRLDRFVAVLDTLGLRNP
                     PYKVITVAGTNGKGSCVAMLESIYWHAGYDVGTFTSPHLWRFNERIRFNGNDAGDAEL
                     VEVFEEAAAALGPVTLSYFEASLVAALALFRRLRPDVVVLEVGMGGRLDATNALDTDC
                     ALIVSIDLDHREWLGDTREAIGREKAGIVRAGKPVVIGDRAVPSSVLEHAAEVGALPC
                     LIGPDFDFRRAESGWRRARDTDAARPLPLPPYGGDEQLANAASCAAVVEALAAALPVG
                     DAALAAGIANAVLRGRFERHDVDGVEWVFDVGHNPAAAAGLEAALRRSPAPNRTWIVF
                     AAMRDKDLKGVVEPFAATAAGWFVARASADRGATGDELATLLESLGAERVEIAADVAA
                     ACAAARAAAAPRDRVVVYGSFHTVGAALEALRLYCAPSPLVDRRSTWTRD"
     gene            2959..3615
                     /locus_tag="VFL84_01910"
     CDS             2959..3615
                     /locus_tag="VFL84_01910"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7607403.1"
                     /translation="MKERLVGAAVLVAIAVWLIPWVLDGPEDGAEAAASSLQLPTAEE
                     PVPMRTQILKLGEAPEPSAGSAAAPTATATQQQAAAAPAKPAADPPKTSTDSPKASAD
                     SPKPAEQAVAEPKREPEREQTVAAAARVADSAATPPKAAGGDWTVQLGSFEDEANAKR
                     MAQRAGTFGYKAEVSASRDGARTLYRVRVGPQATKAAADAAASALRAHGVEARVVAAR
                     "
     gene            3623..4162
                     /locus_tag="VFL84_01915"
     CDS             3623..4162
                     /locus_tag="VFL84_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET7607404.1"
                     /translation="MAGVDYVILLLTLLSILIGVWRGFTTEALSLLTLLLAIGLAWML
                     APGVEPHLGDWSAGGEVRLWTARFIIFVVVLALGGLAQWLARKLIRHTGLSSVDRTLG
                     AIFGFLRAAVVLGLAVLVLRFTELDQEAWWQDARLRPYAERAAEAVKYYAELGSRYLE
                     DHPVPQSAGVRLRAWRPKE"
     gene            4171..5700
                     /gene="purF"
                     /locus_tag="VFL84_01920"
     CDS             4171..5700
                     /gene="purF"
                     /locus_tag="VFL84_01920"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET7607405.1"
                     /translation="MCGLVGIVGHEPVNQALYDALTVLQHRGQDAAGIMTDDGGRLRV
                     RKGNGLVRDVFLERHMLKLAGNVGLGHVRYPTAGSASLSEAQPFYVNSPYGICLAHNG
                     NLVNADELKRLVTQQDHRHLNTSSDSEVLLNVFAHELGKHATNGLKPQQILDAVEALY
                     KRCSGGYAVVAMIIGYGIVAFRDPFGIRPLVLGIRETPNGVEHMVASESVALDAVGFE
                     LERDVLPGEAVFIDKRGYLHSRQTSARRGYTPCIFEFVYFARPDSIIDNLSVYKARLR
                     MGEKLAAKILRTRPDHDIDVVIPIPDTSRTAAMQVAQGLGVKYREGFIKNRYIGRTFI
                     MPEQSARKASVRKKLNAIGLEFQGKNVLLVDDSVVRGTTSQQIIDMAREAGAKKVYFA
                     SAAPPVRYPNVYGIDMPAAGELVAAGRTVEQVQRKIGADWLVYQDLDDLVAAVHHEKA
                     DIGGFDTSCFSGEYVTGDVSRAYLDALEVIRSNSAKARRDAKVRAEESEDDTIQVASG
                     L"
BASE COUNT          879 a         1966 c         2040 g          936 t
ORIGIN      
        1 ctcgcggccg ccgtgagctt cgtcgccggc gcccccgtca tcgtgcatgg ctcgggcagg
       61 accgacgccg gcgttcacgc gctgcatcaa gtcgcgcatt tcgacacggc cgtcgcgcgg
      121 acgccgcggc aatggctgct cggcatcaac tcgaatttgc cggccgatgt cgcgattcgg
      181 tgggtgcgcg aggtgccgga cggcttcgac gcgcgccgct cggcgttgag ccgccgctac
      241 cgttacacgg tgctctcgca gccggctcgg ccggcgctgc tgcgccgccg cgcatggtgg
      301 ctgcgccaag agctcgactg cggcgcgatg acggcggcgg cgagcgcgtg gcttggcgag
      361 cacgacttct cggccttccg cggcgcgggt tgccaggcga aatcgccgat gcgccgcttg
      421 acggccgtgg cgatcgcgcg gcggcggcga ggcgagggca cgctcgtgac gttcgagttc
      481 actgcgaacg ccttcttgca gcacatggtc cgcaacctcg tcggaacgtt ggtcgcggtc
      541 ggcggcggcg agctcacgcc gcagcaggcg gcgcggatcc tcgagcttcg cgatcgccgc
      601 gaagcgggcg tgacggcgcc gcccgagggt ttggcgctcg tcgaggtgct gtacccggcc
      661 gagctcgctc tgccgcgcta cgaggacgag ccgtgatgat gcgccttgag gtctttcggc
      721 tatgatgccc ggagtttcgg gcaagattcg ggtatgagct ggttcgacaa gttgatgcca
      781 tcccggattc ggaccgagaa gcgcaagcac tcggtgccgg aagggctctg ggtcaagtgc
      841 cagggctgct ccgcacagct ctatcgcgcc gagctcaacc gcaatctgca cgtctgcccg
      901 aagtgcggcc atcacttgcg catcggcgcg cgcgagcgtc tcgagatgtt tctcgacgcc
      961 gagtccggct acgagctcgc ggcaaacttg gagccgctcg acccgttgcg cttcaaggac
     1021 acgaagcgct accgcgagcg gctgaaccaa gtgcagcgcg acaccgacga gcgcgacgcg
     1081 ctcgtcgtga tggccggcac gttgaaaagc atgcccgtcg tcgcctgcgc gttcgagttc
     1141 cgcttcatct cgggctcgat gggctcggtc gtcggcgagc gcttcgtgcg cgcggtcgcg
     1201 tattgcattc agcaccagcg cccgctcgtc tgcttctccg cgagcggcgg cgcgcgcatg
     1261 caggaaggct tgtactcgct gctgcagatg gcgaagacga gctcggcgct cgcgcagctc
     1321 gcggccgcga agctgcccta cgtttccgtc atgacggatc cgacgacggg cggcgtttcg
     1381 gcgagcctcg cgctgctcgg tgacatcaac atcgccgagc ccggagcgct gatttgcttc
     1441 gcggggccgc gcgtcatcga gcagacgacg ggcgaaacgc tgcccgaagg tttccagcgc
     1501 gccgagttcc tgctcgagaa gggcgcgatc gacatgatcc tcgatcgccg gcactcgcgc
     1561 gatcagatcg cggcgctgct cgcgaagctg tcctcgcgcc ccgcgttgtc ctgagccagg
     1621 aggctcgttt cgcgtgacga ctccgccgcg cggattgacg ctcgatcatt ggctcgcctg
     1681 gttcgagacc gtccatccga agcggatcga cctgcggctc gaccgattcg tcgcggtgct
     1741 cgacacgctc ggcttgcgca accctcccta taaagtcatc acggtcgccg gcacgaacgg
     1801 caagggctcg tgcgtcgcga tgctcgagag catctactgg cacgccggct atgacgtcgg
     1861 cacgttcacg tcgccgcacc tgtggcgctt caacgagcgc attcgcttca acggcaacga
     1921 cgcgggcgac gccgagctcg tcgaggtgtt cgaggaggcc gcggccgcgc tcggcccggt
     1981 cacgctgtcg tacttcgagg cgtcgctcgt cgccgcgctc gcgctgttcc gccgcttgcg
     2041 tcccgacgtc gtcgttctcg aggtcggaat gggcggccgg ctcgacgcga cgaacgcgct
     2101 cgacacggac tgcgcgctga tcgtcagcat cgacctcgat caccgcgaat ggctcggcga
     2161 cacgcgcgag gcgatcggcc gcgagaaggc cggcatcgtg cgcgcgggca agcccgtcgt
     2221 catcggcgat cgcgccgtgc cgtcgagcgt gctcgagcac gcggccgaag tcggcgcgct
     2281 gccgtgcttg atcggccccg acttcgattt ccggcgcgcc gagtcgggct ggcgcagggc
     2341 gcgcgatacg gacgcggcgc ggcctttgcc gttgccgccc tacggcggcg acgagcagct
     2401 cgcgaacgcg gcgtcgtgcg ccgccgtcgt cgaagcgctg gctgcggcgc tgcccgtcgg
     2461 cgacgcggcg ctcgcggccg gaatcgcgaa cgcggtgctg cgcggccgct tcgagcgcca
     2521 cgacgtcgac ggcgtcgagt gggtcttcga cgtcggccac aatcccgccg ccgcggccgg
     2581 cctcgaggcc gccttgcgcc gctcacccgc gccgaatcga acgtggatcg tgttcgcggc
     2641 gatgcgcgac aaggatctca aaggcgtcgt cgagccgttc gccgcgacgg cggccggctg
     2701 gttcgtcgcg cgagcgagcg ccgatcgcgg cgcgacgggc gacgagctcg ccacgttgct
     2761 cgagagcctc ggcgccgagc gagtcgagat cgcggccgac gtcgcggccg cgtgcgccgc
     2821 ggcgcgcgca gcggcggctc ctcgcgaccg cgtcgtcgtc tacggctcct ttcataccgt
     2881 cggcgccgcg ctcgaagcgc ttcggctata ctgcgcaccc tccccattgg ttgaccgacg
     2941 ctcgacatgg accagggatt gaaggagcga ctcgtcgggg cggccgtgct ggtcgcaatc
     3001 gccgtctggc tgattccgtg ggtgctcgac ggccccgagg acggggccga agcggccgca
     3061 agctcgctgc aattgcctac ggccgaggag ccggtgccga tgcggacgca gatactcaag
     3121 ctcggcgaag ctccagagcc gagcgcgggc tccgccgccg cgccgaccgc gacggcgacg
     3181 cagcaacagg cggcggccgc gccggcgaag ccggcggccg acccgccgaa gacctcgacc
     3241 gattcgccga aggcctcggc cgattcgccg aagcccgcgg agcaggccgt ggccgagccg
     3301 aagcgcgagc ccgaacgcga gcagacggtt gccgccgcgg cccgcgtggc ggacagcgcg
     3361 gctacgccgc cgaaggcggc cggcggggat tggaccgtgc agctcggcag cttcgaggac
     3421 gaagccaacg cgaagcgcat ggcgcagcgc gccgggacgt tcggttacaa ggccgaggtt
     3481 tcggcctcgc gcgacggcgc gcggacgttg taccgcgtgc gagtcgggcc gcaggcgacg
     3541 aaggcggcgg ccgatgcggc ggcgtcggcg ctgcgtgccc acggcgtgga agcccgcgtg
     3601 gtggctgcgc gctgagcggc gcatggcggg cgtcgactac gtaatcttgc tgttgacgct
     3661 gctctcgata ttgataggtg tctggcgcgg gttcacgacg gaggcgctgt cgctgctgac
     3721 gctgctcctg gccatcggcc tcgcgtggat gctcgcgccc ggcgtggagc cgcacctcgg
     3781 ggactggagc gcgggcggcg aggtcaggct gtggacggcg cggttcatta tttttgtcgt
     3841 cgtgctcgcg ttgggtggcc ttgcgcagtg gcttgcgaga aaattgatcc ggcatacggg
     3901 gctctcgagc gtcgaccgga cgctcggcgc gatattcggg ttcttgcgcg cggccgtcgt
     3961 gctgggcctc gcggtgctgg tcctgcggtt cacggagctc gatcaggagg cctggtggca
     4021 agacgcgcgt ttgaggccgt acgccgagcg cgccgcggag gccgtgaagt actacgcaga
     4081 gctcggcagc cggtacttgg aagatcatcc cgtgccccaa tctgcgggtg tccggctcag
     4141 ggcgtggcgt cctaaggaat aggtgctgat atgtgtggat tggtcggaat cgtcggtcac
     4201 gagcccgtga atcaagcgct ttacgatgcg ctgacggtgc tgcaacaccg cgggcaggac
     4261 gccgccggca tcatgaccga cgacggcggg cggctgcgcg ttcgcaaagg caacggcctc
     4321 gtccgcgacg tgtttctcga gcggcacatg ctgaagctcg ccggcaacgt cggtttgggg
     4381 cacgtccgct atccgacggc cggcagcgcg agcctctctg aagcgcagcc gttctatgtg
     4441 aactccccgt acggcatctg cctcgcgcac aacggcaacc tcgtgaacgc cgatgagctg
     4501 aagcggctcg tgacgcagca ggatcaccgc cacctgaaca cgagctcgga ttccgaagtc
     4561 ctgctgaacg tattcgcgca cgagctcggc aagcacgcga cgaacggctt gaagccgcag
     4621 caaatcctcg acgccgtcga ggcgctctac aaacgctgct cgggcggcta cgccgtcgtc
     4681 gcgatgatca tcggctacgg tatcgtcgcg ttccgcgacc cgttcggcat ccggccgctc
     4741 gtgctcggca ttcgcgagac gccgaacggc gtcgagcaca tggtcgcctc ggagagcgtc
     4801 gcgctcgacg cggtcgggtt cgagctcgag cgcgacgtcc tgcccggcga ggccgtgttc
     4861 atcgacaagc gcggctatct gcacagccgt cagacttcgg cgcgccgcgg ctacacgccg
     4921 tgcatcttcg agttcgtgta cttcgcgcgg cccgactcga tcatcgacaa tctctccgtc
     4981 tacaaagcgc gcttgcgcat gggcgagaag ctcgccgcga agatcctgcg cacgcggccc
     5041 gatcacgaca tcgacgtggt gattccgatc ccggacacgt cgcgcacggc cgcgatgcaa
     5101 gtcgcgcaag gcttgggcgt caagtaccgc gagggcttca tcaagaaccg ctacatcggc
     5161 cgcacgttca tcatgccgga gcagtcggcg cgcaaggctt cggtgcggaa gaagctgaac
     5221 gcaatcgggc tcgagttcca gggcaagaac gtgctgctcg tcgacgactc cgtcgtgcgc
     5281 ggcacgacgt cgcagcagat catcgacatg gcgcgcgagg ccggcgccaa gaaggtctat
     5341 ttcgcgtcgg ccgcgccgcc ggtccgctat ccgaacgtct acggcatcga catgccggcg
     5401 gccggcgagc tcgtcgccgc gggccgcacc gtcgagcagg tccagcgcaa gatcggcgcc
     5461 gactggctcg tctaccaaga cctcgacgat ctcgtcgcgg ccgtgcacca cgagaaggcc
     5521 gacatcggcg gcttcgacac gtcgtgcttc tccggcgagt acgtcaccgg cgacgtgagc
     5581 cgcgcgtacc tcgacgcgct cgaggtcatt cgctcgaact ccgcgaaagc gcgccgcgac
     5641 gcgaaggtgc gcgccgagga gtccgaggac gacacgatcc aagtcgcaag cggcctctag
     5701 ccaccctcaa aaaggggaca gatctatttt ctcggacgca acgcgcaaaa aggggacgga
     5761 tctattttct tgggaaaacg cgtttgtccc gcggggctct tctgaaaata gatccgtccc
     5821 c
//
LOCUS       DASSNN010000106         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_149526, whole genome shotgun sequence.
ACCESSION   DASSNN010000106 DASSNN010000000
VERSION     DASSNN010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149526"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="VFL84_01925"
     CDS             complement(<1..1541)
                     /locus_tag="VFL84_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET7607406.1"
                     /translation="MSTIDKLIGRMSLAEKLGQLTMTSASYAVTGPVIAGDSTDSIRD
                     GSLGNLLNLVGAKHVHEMQRIAVEESRLGIPLLIGFDVIHGHRTIFPVPLAEAATFDA
                     NVWELTAREAARETAADGLAMTFAPMLDVSRDLRWGRTVEGPGEDPWLAEQISRAKVR
                     GFQGADLADPQSVAATAKHFCAYGPVAGGRDYASVDISERTLLEVHLPAFAAAVEAGV
                     AAIMPAFTELGGIPMTMHKPLLRDYLRGELGFDGVIVSDYNAIRELLHHGVAGDIVEA
                     AVLALKAGVDIDMMADAYRKGLPVALERGLVTIEEIDECVLRVLRLKERLGLFDDPYR
                     RGSSTETADAIAARRRLARDVAAKSLVLVKNSADALPLTAGVRRVSVIGPLADAWREM
                     RGPWAAAGYEEPSVTVLAGLRAALPAADVVHVEGVTIAGAETSHIAAAVEAARGADVV
                     VLCLGEAAEMSGEAASRAFPELPGVQRALAEAVLDRARGDEVPVVVVLFSGRPLIVPW
                     LFERA"
     gene            1618..2976
                     /locus_tag="VFL84_01930"
     CDS             1618..2976
                     /locus_tag="VFL84_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HET7607407.1"
                     /translation="MRRPESRKRAKRSRSRSTGAAAPRGGAAEARDPAKLALLPDDAL
                     LDAVQRRTFRYFWEAAHPVSGLAFDRRTAGVRANEADPTTIGGSGFGVMALIVAVERG
                     WITRDAVLDRLDRMLDLLSRARCYHGAFPHFMNGVTGEAIPFGRKDDGADIVETSLLM
                     MGLLSARQYFDRDTPQEASVRSRITALWTDVEWSWFTRGRDVLYWHWSPYNGWAMEHA
                     ILGWNECLVTYLLAAASPRYPIDSTVYHNGFASGPGFCNGKPWYDIELPLGMPYGGPL
                     FFAHYSFCGIDPRGLTDRYADYWQQNVRHVRINRAHCIANPGKFEGYGEACWGLTASD
                     DPAGYSAHAPDNDNGTISPTAALASLPYAPAEVMTVLRHFLRRHGKRVWRELGFVDAF
                     CESQSWYADTFLAIDQGPIIVMIENHRSGLLWKLFMAVPEVRAGLRRLGFSSPHLAKA
                     GP"
BASE COUNT          500 a         1041 c         1005 g          465 t
ORIGIN      
        1 tcggcacgct cgaagagcca tgggacgatc aacggccggc cgctgaaaag gacgacgacg
       61 acgggcacct cgtcgccgcg ggcccggtcg agcacggcct ccgcgagcgc gcgttgcacc
      121 ccgggcagct cggggaacgc gcgcgacgcg gcttccccgc tcatctcggc cgcctcgccg
      181 aggcagagca cgacgacgtc ggcccccctc gccgcttcga ccgccgcggc gatgtgcgat
      241 gtctcggcgc ccgcgatcgt cacgccttcg acgtgcacga cgtcggcagc cggcaacgcc
      301 gcgcgcagcc cggcgagcac cgtgacgctc ggctcctcgt agcccgcggc ggcccaggga
      361 ccgcgcattt cgcgccacgc gtcggcgagc ggcccgatca cggagacgcg gcgaacgccg
      421 gcggtgagcg gcaaagcgtc ggcggaattc ttgacgagca cgagcgactt cgccgcgacg
      481 tcgcgcgcga gccgccgccg ggccgcgatc gcatcggcgg tctccgtgct cgagcctcgc
      541 cggtacggat cgtcgaacaa gccgagacgc tccttgagac gcagcacgcg cagcacgcac
      601 tcgtcgatct cctcgatcgt cacgagcccg cgctcgagcg cgaccggcaa acctttgcga
      661 taggcgtcgg ccatcatgtc gatgtcgacg ccggctttca gcgcgagcac ggccgcctcg
      721 acgatgtcgc cggccacgcc gtgatgcagc agctcgcgaa tcgcgttgta gtcgctgacg
      781 atgacgccgt cgaatccgag ctcgccgcgc aggtaatcgc gcagcagcgg cttgtgcatc
      841 gtcatcggaa tgccgccgag ctccgtgaac gccggcatga tcgccgcgac gccggcttcg
      901 acggccgcgg cgaatgcggg cagatgcact tcgagcagcg tgcgctcgga gatgtcgacg
      961 gacgcgtagt cgcgcccgcc ggcgacgggc ccgtaggcac agaagtgctt cgccgtcgcg
     1021 gcaaccgact gcggatcggc gagatccgcg ccctggaatc cgcgcacctt cgcgcgcgaa
     1081 atctgctcgg cgagccacgg atcctcgccc gggccttcga ccgtgcgccc ccagcgcaga
     1141 tcgcgcgaga cgtcgagcat cggcgcgaac gtcatcgcga gaccgtcggc tgccgtctcg
     1201 cgcgccgcct cgcgcgccgt gagctcccat acgttcgcat cgaacgtcgc ggcctcggcg
     1261 agcggcacgg gaaagatcgt gcggtgcccg tgtatcacgt cgaagccgat caagagcggt
     1321 atgccgagcc gcgattcctc gacggcgatg cgctgcatct cgtgcacgtg cttcgctccg
     1381 acgaggttca acaagttgcc gagagatccg tcgcggatcg agtcggtcga atcgcccgcg
     1441 atgacgggcc ccgtcacagc gtacgacgcg gaagtcatcg tcaactggcc cagcttctcc
     1501 gccaggctca ttcggccgat gagcttgtca atcgtgctca tgaagacaat catgattcat
     1561 tcagcgaagg aatcgcaggc gacatgcaag atgggcgcca gaagaaaggg aggtcacatg
     1621 cgacggccgg aatcccgtaa gcgcgcgaaa cgaagccgct cccgcagcac gggcgcggcc
     1681 gcgccgcgcg gcggggcggc cgaggcccgc gaccccgcga agcttgcgct gctgcccgac
     1741 gacgcgctgc tcgatgccgt gcagcggcga acgttccgct atttctggga agcggctcat
     1801 cccgtcagcg ggctcgcatt cgaccgccgc accgcgggcg tgcgcgcgaa cgaagcggat
     1861 ccgaccacga tcggcggctc gggcttcgga gtcatggcgc tgatcgtcgc agtagagcgc
     1921 ggctggatca cgcgcgatgc ggtgctcgat cggctcgacc gcatgctcga tctgctctct
     1981 cgcgcgcgct gctaccacgg cgcgtttccg cacttcatga acggcgtcac cggagaggcg
     2041 attccgttcg gacgcaaaga cgacggcgcc gatatcgtcg agacgtcgct gctgatgatg
     2101 ggcctgctct ctgcgcgtca gtacttcgat cgcgatacgc cgcaagaagc gtcggtccgc
     2161 tcgcgcatca cggcgctgtg gaccgacgtc gaatggagct ggttcacgcg aggccgcgac
     2221 gtcctctact ggcactggag cccgtacaac ggctgggcca tggagcatgc gattctcggc
     2281 tggaacgagt gcctcgtgac gtatctactg gcggccgcgt cgccgcgcta tcccattgat
     2341 tcgacggtct atcacaacgg gttcgccagc gggcccggat tttgcaacgg caaaccttgg
     2401 tacgacatcg agttgcctct cggcatgccg tatggagggc cgctgttttt cgcgcactac
     2461 tccttctgcg gaatcgatcc ccgcgggctc acggaccgct acgccgacta ttggcaacag
     2521 aatgttcgtc acgtccgtat caaccgcgcg cactgcattg cgaatccggg caagttcgag
     2581 ggctacggcg aagcgtgctg gggcctgacg gccagcgacg acccggccgg ttactccgcg
     2641 cacgcacccg acaacgacaa cggcacgatc agcccgaccg cggcgctcgc gagcctgccg
     2701 tacgcgccgg ccgaagtcat gacggtgctg cggcattttc tgcggcgcca tgggaaacgg
     2761 gtctggcgcg agctcggctt cgtcgacgcg ttctgcgaga gccagagctg gtacgccgat
     2821 acgtttctcg cgatcgacca gggcccgatc atcgtcatga tcgagaacca ccggtcgggg
     2881 ctcttgtgga agctcttcat ggcggtgccc gaagtgcggg ccgggctgcg gcgactgggg
     2941 ttctcgagcc cgcatctggc aaaagccggg ccgtgaccga aaccctcgag cagtggctcg
     3001 gtcgcgagca c
//
LOCUS       DASSNN010000107         3640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1030798, whole genome shotgun sequence.
ACCESSION   DASSNN010000107 DASSNN010000000
VERSION     DASSNN010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030798"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2064
                     /locus_tag="VFL84_01935"
     CDS             <1..2064
                     /locus_tag="VFL84_01935"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HET7607408.1"
                     /translation="LLPAALAFGLLVAVTAAAGVLAVLQDARALAVFGIFGGFLAPVL
                     VSTGSNNHVALFSYYALLDLAILGIAWFKAWRVLNVLGFLFTFGIGTMWGVNYYVPEN
                     LATTEPFLVLFTAMYLVIPVLFAMRVEPPRLRGFVDSTLMFGTPIVAFGLQSQLVGDT
                     KYGLAISAVALAAAYIGMATFLFRTRRGELRVLVESQLALGVAFLTVAVPLALDDALW
                     TSAAWALEGAALVWLAFRQQRRLALVAGVALQALSAAAYIVKSPVAAEWPVLNGPFLG
                     ALVLAFAGFFSARLFDPERENRTGHGALTEAFAKAASIVLLLWAAAWWLFAGIDEIDR
                     SVGYDIELAALLGFAAGTALLAIALAARLRWPRLNWLSLALWPSAGLLFAVGLLGVAH
                     PAADFGWLAWPVAVGAMLAVLRAREALFGRLAGALHVLAYWLAAGLLLSETHWLVERA
                     TQGVWPEAAVLALGAVFVLATLRAPAAVAWPFAAHARAYLQAACGALLAALTASALAL
                     NVESSGEALPLPYLPLVNPLELASVLVLVALLMWLEAVGRETPTLEAAARARPAVAGA
                     AVWFLSTMTVARCVHHWAHVPYDLDSLAASTTFQSALSIVWGIAGLAAMVVGARSARR
                     GVWLAGGALMSVVVVKLFLVDLGNTGTLARVVSFLGVGVLLLVVGYFAPVPPRLPAEA
                     SAARS"
     gene            2076..3434
                     /locus_tag="VFL84_01940"
     CDS             2076..3434
                     /locus_tag="VFL84_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HET7607409.1"
                     /translation="MQKGRTLLRASAAAAGAACAAAAMALDPLDFVAGWPIEAPADAK
                     VFDVPLTAEVYAASQGIEQLAVLDANGEPQGFFRRSPPSNEPTERRVVLEASPLYEGG
                     SAVAPSVGVTTGQRGTTVTVTPGSASSTKIVGFVLDAREVDVAPNALELDWRALPQPF
                     LLDVAVEQSEDLTHWRGVGRASVAALSVGGTEARHARVPVRAGAGGYLRVTASGAVAD
                     WQLLRATLISVATEPAMPQSVRIAPLPADAAPADGAPGALYFDAGGPLPVSTVTLAFG
                     DNDGWVQADVAAARSLEGPWIPVAHRALIYALSFEGREFTSDALAVGRHEARYWRFVP
                     AAPPRGERMTLVLEFPQESLRVAAGGGAPYLLAAGTLADEAGPDATLASVWSRLAPPE
                     LAVPLATLGQRRELGGAAALVAPRVFPWRTAALWSVLILGVLVVGAMAVRLAKEMQKP
                     ST"
BASE COUNT          380 a         1283 c         1335 g          642 t
ORIGIN      
        1 ctcttgccgg ccgcgctcgc gttcggcttg ctagtggcgg tgaccgcggc ggcgggcgtg
       61 ctggccgtgc tgcaagacgc gcgcgcgttg gcggtcttcg gcatcttcgg cggcttcctc
      121 gcgccggtgc tcgtgtcgac ggggtcgaac aatcacgtcg cgctgttcag ctattacgcg
      181 ctgctcgatc tcgccatcct cggcatcgcg tggttcaagg catggcgcgt gctgaacgtg
      241 ctcggtttcc tgttcacgtt cggcatcggc acgatgtggg gagtcaacta ctacgtgccc
      301 gagaacctcg cgacgaccga gccgttcctc gtgctgttca cggcgatgta cctcgtcatc
      361 ccggtgctgt tcgcgatgcg cgtcgagccg ccgcgcttgc gcggtttcgt cgacagcact
      421 ctgatgttcg gcacgccgat cgtagcgttc gggctccaga gccagctcgt tggcgacacg
      481 aagtacgggc tcgcgatcag cgcggtcgcg ctcgccgccg cttacatcgg gatggcgacg
      541 ttcctattcc gcacacgtcg cggcgagctg cgcgtgctcg tcgagtcaca gctcgcgctc
      601 ggtgtcgcgt tcctgaccgt cgcggtgccg ctcgcgctcg acgacgcgct ctggacctcg
      661 gccgcttggg cgctcgaggg ggcggctctc gtctggctcg cgtttcgcca gcagcgccgg
      721 ctcgcgctgg tcgcgggtgt agccctgcag gctttgtctg ccgcggccta catcgtgaag
      781 tcgccggtcg ccgcggaatg gccggtgctg aacggtcctt ttctcggcgc gctcgtcctc
      841 gcgttcgccg gtttcttctc ggctcgcttg ttcgatcccg agcgcgagaa ccgaacgggg
      901 catggcgccc tcacggaggc gttcgcgaag gcagcctcga tcgtgctgct gctctgggcg
      961 gcggcgtggt ggctcttcgc gggcatcgac gagatcgacc gaagcgtcgg ctacgacatc
     1021 gagcttgcgg cgctgctcgg gttcgccgcg ggcacggcgt tgctcgcgat cgcgctcgcc
     1081 gcgcggttgc gctggccccg gctgaactgg ttgagccttg cgctgtggcc gtcggccggc
     1141 ctgctgttcg cggtcgggct gctcggcgtc gcgcatcccg ccgcggattt cggctggctc
     1201 gcatggccgg tcgccgtcgg cgcgatgctc gcggtgctgc gcgcgcgcga ggcgctgttc
     1261 ggacggctcg ccggcgcgtt gcacgtgctc gcgtactggc tcgcagcggg cttgctgctc
     1321 tcggagacgc actggctcgt cgagcgcgcc acgcaagggg tctggcccga ggccgccgtg
     1381 ctcgcgctcg gcgcggtatt cgtgctcgcg acgctgcgcg cgccggcggc cgtcgcctgg
     1441 ccgttcgcgg cgcacgctcg cgcctatctg caagccgctt gcggcgcgct gctcgccgca
     1501 ctgaccgcga gcgcgctggc gctgaacgtc gagtcgtccg gcgaagcttt accgctgccg
     1561 tatctgccgc tcgtgaatcc gctcgagctc gcgagcgtcc tcgtgctcgt cgcgctgctc
     1621 atgtggctcg aagcggtcgg ccgcgagacg ccgactctcg aggccgccgc ccgcgcgcgc
     1681 cccgcggtcg ccggcgccgc ggtctggttc ctctcgacga tgacggttgc gcgctgcgtg
     1741 catcattggg cgcacgtgcc gtacgacctc gacagcctcg ccgcttcgac gacgttccag
     1801 agcgcgctgt cgatcgtgtg gggtatcgcg ggcctcgccg cgatggtcgt cggcgcgcga
     1861 tcggcgcgcc gcggagtctg gctcgccggc ggcgcgttga tgagcgtcgt cgtggtgaag
     1921 ctgttcctcg tcgacctcgg caacacgggc acgctcgcgc gcgtcgtttc gttcttgggc
     1981 gtcggcgtgc tgctgctcgt cgtcggctac ttcgcgccgg tgccgccgcg cctgccggca
     2041 gaagcgagcg cagcgcgctc gtgaaggaga gacccatgca gaagggcaga acgctgttac
     2101 gagcttcggc cgcggccgcc ggagcggcat gcgccgcggc tgccatggcg ctcgacccgc
     2161 tcgacttcgt cgccggctgg ccgatcgaag cgccggccga cgcgaaagtg ttcgacgtgc
     2221 cgctgacggc cgaagtctat gcggcctcgc agggcatcga gcagctcgcg gtcctcgacg
     2281 cgaacggcga gccgcaaggg ttcttccgcc gcagcccgcc gtcgaacgag ccgaccgagc
     2341 gtcgcgtcgt gctcgaggcg tcgccgctgt acgagggcgg ctccgccgtg gcgccgagcg
     2401 tcggcgtcac gaccggccaa cgcggcacga cggtgaccgt cacgccgggc tcggcgagct
     2461 cgacgaagat cgtcggcttc gtgctcgacg cgcgcgaagt cgacgtcgcg ccgaacgcgc
     2521 tcgagctcga ttggcgcgcg ttgccgcagc cgttcctgct cgacgtcgcg gtcgagcaga
     2581 gcgaggacct cacgcattgg cgcggcgtcg gccgggcgtc ggtcgcggcg ctgtcggtcg
     2641 gcggcacgga ggcgcgccac gcgcgcgtgc ccgtgcgcgc cggcgcgggc ggttatctgc
     2701 gcgtcacggc gagcggcgcg gtcgcggact ggcagctctt gcgcgcgacg ctgatcagcg
     2761 tggcgaccga gccggcgatg ccgcagagcg tgcgaatcgc accgttgccc gcggacgccg
     2821 cgcccgccga cggcgcgccc ggcgcgctgt acttcgacgc gggcgggccg ctgcccgttt
     2881 cgaccgtcac gctcgcgttc ggcgacaacg acggctgggt ccaggccgac gtcgcggctg
     2941 cgcgctcgct cgaaggaccc tggattcccg tcgcgcaccg cgcgctgatc tacgcgttgt
     3001 cgttcgaggg gcgcgagttc acgagcgacg cgctcgcggt cggccggcac gaggcccgtt
     3061 actggcgctt cgtgccggcg gcgccgccgc gcggcgagcg catgacgctc gttctcgagt
     3121 ttccgcagga gagcttgcgc gtcgcggccg gaggcggcgc tccgtatctg ctcgcagccg
     3181 gcacgttggc cgacgaggcg ggccccgacg caacgttggc gtcggtctgg tcgaggcttg
     3241 cacctccgga gctggccgtg cccctggcga cgctcggtca gcgccgcgag ctcggcggag
     3301 cagcggcgct cgtcgcgccg cgcgtcttcc cgtggcgcac cgcggcgcta tggagcgtgt
     3361 tgatcctggg cgtgcttgtg gttggggcga tggccgttag actcgcaaag gagatgcaga
     3421 agccctcgac ctgaggttcg cctggccaac cggaatcgcc cgtcgttcgt tcgccgcgtc
     3481 gtgcgccgat tggtgctcgc atgcctcgcg ctcgtcgcgc tgtcggccgg cgccgtgctc
     3541 gcgctgcgct gggtcgaccc gcccgtcacg gccgtgatgc tgctacagcc cgggtccgtg
     3601 ggcgagctcc actacgtctg ggccggtcgc cgcgcgatcg
//
LOCUS       DASSNN010000108         4446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1030836, whole genome shotgun sequence.
ACCESSION   DASSNN010000108 DASSNN010000000
VERSION     DASSNN010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030836"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1327
                     /gene="trpB"
                     /locus_tag="VFL84_01945"
     CDS             77..1327
                     /gene="trpB"
                     /locus_tag="VFL84_01945"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET7607410.1"
                     /translation="MEKKEREALLADLLAGKLPDKNGRFGPFGGRYVPETLMPAIERL
                     MHGVEHILPSAEFQAALKRELSSWAGRPTALTPVPRLSERWGADVWLKREDLAHTGAH
                     KINNGIGQALLAKALGAKRVVAETGAGQHGVASAAACARVGLPCTVYMGEIDVARQAP
                     NVDRMRRFGATVVAVKSGDRTLRAAIDEAIRDWVSDPVGTYYLLGSAIGPHPYPYLVR
                     QLQSVIGREARAQMLDAAGGLPDAVFACVGGGSNAIGMFHGFLADAAVKLFGVEAGGR
                     GPNLGDHSATLSYGKPGVLHGTQTMLLYDEHGQIQETHSISAGLDYPGVGPEHALLQA
                     IGRVEYVTASDDETLAALEELTACEGILPALESSHALAGAKRWAAKNPRARVLIGLSG
                     RGDKDMPILAKYPLAAHEDRHAAA"
     gene            1314..2084
                     /gene="trpA"
                     /locus_tag="VFL84_01950"
     CDS             1314..2084
                     /gene="trpA"
                     /locus_tag="VFL84_01950"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET7607411.1"
                     /translation="MQPRDRIRHALARDLVKRGHPALVGYITAGFPEPSEFLKILKAV
                     AGAADAVEIGVPFSDPMADGVTIQRSSERAIEKGVSLRFILDELARRDFELTAPVLLM
                     SYLNPLLAFGYAALARRAAEVGVAGFIVPDLPYEESAPLAAALDPHGLALVQMVTPAT
                     PPERLRRLCAASQGFVYAVTRTGVTGDAALPPGTAEYLSAVKAASPLPVCAGFGVRRA
                     DQVAMLGAHADGVIVGSALVEVLERGADPAVFLRSLRT"
     gene            2086..3033
                     /locus_tag="VFL84_01955"
     CDS             2086..3033
                     /locus_tag="VFL84_01955"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET7607412.1"
                     /translation="MRWLDRIVIACVAVVVVLNLLPLGARLYWTLELTTHFRLQYLAL
                     TALMLLLAALRRRWIVVAALAVAGTVSAAAVWPYVPRAGGAVVAAPQARTLKVLTVNV
                     SYRQFLPRRLLELVRDAGPDIVVVQELTPHAERVLASLDTEFPYFRKFPADGAYGIGL
                     WSRHELESSETIALGRLPAIEARVRGPSGSFTVIGVHLSAPVTRRRAAARNQQLVELA
                     ARSAAVAGPLIVAGDFNTTPYSPYFADWLEASALTDSRRGRTLSPSWPTMLQLVGIPI
                     DHVAVNDGFTILSHRRLPNFESDHFGALVEVALRQAEHP"
     gene            3030..3767
                     /locus_tag="VFL84_01960"
     CDS             3030..3767
                     /locus_tag="VFL84_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HET7607413.1"
                     /translation="MSAPPTACVLIIGNEILSGKTQDTNLQFLGFELAKLGIRLAEAR
                     VVRDEPAAIIRHLNEARRDFTYVFTTGGIGPTHDDITAECVAQAFGVPLVRSEEAVER
                     LKRGGRPLNEARLKMARVPRGAQLIENPVSNAPGFRIENVFVFAGIPSIARAMFTSAI
                     PMLVHAPPILSASVDVHLKEGDFAESLEQIQSRHPTVEIGSYPFNRDGRFGATLVARG
                     TDRALLANVVDEIVAAMTKLGGETHVL"
     gene            3907..>4446
                     /locus_tag="VFL84_01965"
     CDS             3907..>4446
                     /locus_tag="VFL84_01965"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HET7607414.1"
                     /translation="MRKWKFEWRGKLPSSAYEELEVWVSVLLMLAAALLLYATLKAQA
                     ARAAEFPRDIGAPTLLFQDGSGSYSAAAPLETDVRLSVAGVVARVRVAQRFRNVGSTY
                     LEAVYVLPLPDDAAVDRLSMKIGERVIEGEIHERAAAERIYGRARAAGQRASLVRQTS
                     ANLFTTAVANIAPGEHIDIT"
BASE COUNT          641 a         1553 c         1566 g          686 t
ORIGIN      
        1 gcgcgcgcgc ggcgagaaag atccggacaa gattcgtgag ttcgtcgccc gcgtccgggc
       61 gagcggggct tgaagcatgg aaaagaagga acgagaggcg ctgctcgccg acctgctcgc
      121 gggcaagctg cccgacaaga acggccgctt cggcccgttc ggcggccgtt acgtgcccga
      181 aacgttgatg cccgcgatcg agcggctcat gcacggcgtc gagcacatcc tgccgtcggc
      241 ggaattccaa gccgcgctaa aacgcgagct ttcgagctgg gccgggcgcc ccacggcgct
      301 gacgccggtg ccgcgcttga gcgagcgctg gggcgccgac gtgtggctca agcgcgagga
      361 cctcgcgcac acgggcgcgc acaagatcaa caacggcatc ggccaggcgc tgctcgcgaa
      421 agcgctcggc gccaagcgcg tcgtcgccga gacgggcgcg ggtcagcacg gcgtcgcgtc
      481 ggccgcggca tgcgcgcgcg tcggcttgcc gtgcacggtg tacatgggcg agatcgacgt
      541 cgcgcggcag gcgccgaacg tcgaccgcat gcggcggttc ggcgcgaccg tcgtggccgt
      601 caagtccggc gatcgcacgc tgcgcgccgc gatcgacgag gcgatccgcg actgggtctc
      661 ggacccggtc ggcacttact acttgctcgg ctcggcgatc gggccgcatc cgtatccgta
      721 tctcgtgcgg cagctccagt ccgtcatcgg ccgcgaagcg cgagcgcaga tgctcgacgc
      781 cgcgggcggc ttgccggacg ccgtcttcgc ttgcgtcggc ggcggctcga atgcgatcgg
      841 catgttccac ggcttcttgg ccgacgcggc ggtcaagctc ttcggcgtcg aagcgggcgg
      901 ccgcgggccg aatctcggcg atcattcggc gacgctgtcg tacggcaagc ccggcgtgct
      961 gcacggcacg cagacgatgc tgctctacga cgagcacggc cagattcagg agacgcactc
     1021 gatctcggcg ggcctcgact atcccggcgt cgggcccgag cacgcgctgc tgcaagcgat
     1081 cggccgcgtg gaatacgtga cggcgagcga cgacgagacg ctcgcggcgc tcgaggagct
     1141 cacggcctgc gaaggcatcc tgcccgcgct cgagagctcg cacgcgctcg cgggcgcgaa
     1201 acgctgggcc gcgaagaacc cgcgcgcgcg agtgctgatc gggctctcgg gccgcggcga
     1261 caaggacatg ccgatcctcg cgaagtaccc gctcgccgcg cacgaggacc ggcatgcagc
     1321 cgcgtgatcg aatccgtcac gcgctcgcgc gcgatctcgt gaagcgcggg caccccgcgc
     1381 tcgtcggcta catcaccgcg ggctttcccg agccgagcga gttcttgaaa atcctgaaag
     1441 cggtggcggg cgccgcggac gccgtcgaga tcggcgtgcc gttctcggac ccgatggccg
     1501 acggcgtcac gatccagcgc tcgagcgagc gcgcgatcga gaaaggcgtg agcctccgct
     1561 ttattctcga cgagctcgcg cggcgcgact tcgagctcac ggcgcccgtg ctgctcatga
     1621 gctatctgaa tccgctgctc gcgttcggct acgcggcgct cgcgcggcgc gcggccgaag
     1681 tcggcgtcgc cggattcatc gtgcccgacc tgccgtacga ggaaagcgcg ccgctcgcgg
     1741 cggcgctcga cccgcacggg ctcgcgctcg tgcaaatggt gacgccggca acgccgcccg
     1801 agcggctgcg ccgcttgtgc gccgcgagcc agggattcgt ctacgcggtc acgcgcacgg
     1861 gcgtgaccgg cgacgccgcg ttgccgcccg ggacggccga gtatctgtcg gccgtcaaag
     1921 cggcctcgcc gctgcccgtg tgcgcgggct tcggcgtgcg gcgcgccgat caggtcgcga
     1981 tgcttggcgc gcatgccgac ggcgtcatcg tcggctcggc gctcgtcgaa gtgctcgagc
     2041 gcggcgccga tcccgccgtg ttcctgcggt cgctgcgaac ctgacatgcg ctggctcgat
     2101 cggatcgtca tcgcctgcgt tgcggtcgtc gtcgtcttga acctgttgcc gctcggcgcg
     2161 cggctctact ggacgctcga gctcacgacg catttccgcc tgcagtacct cgcgctgacc
     2221 gcgctgatgc tgctgctcgc ggcgctgcgg cggcggtgga tcgtcgtcgc ggcgctcgcc
     2281 gttgcgggca ccgtcagcgc ggcggccgtg tggccgtacg tgccgcgcgc gggtggcgcg
     2341 gttgtcgccg ctccccaagc gcggacgctg aaggtgctga cggtcaacgt ctcgtatcgc
     2401 cagttcctgc cgcggcggct gctcgagctc gtgcgcgacg ccgggcccga catcgtcgtc
     2461 gtgcaggagc tgacgccgca cgccgagcgc gtgctcgcga gcctcgatac cgagtttccg
     2521 tacttccgca agtttccggc cgacggcgcg tatggcatcg gcctgtggtc gcggcacgag
     2581 ctcgaatcga gcgagacgat tgcgctcggc cgcctgcccg cgatcgaggc gcgcgtgcgc
     2641 gggccgtccg gctcgttcac cgtcatcggc gtgcacttga gcgcgcccgt gacgcgccgg
     2701 cgtgccgccg cgcgcaatca gcagctcgtc gagctcgctg cgcgcagcgc ggccgtcgcc
     2761 gggccgctga tcgtggccgg cgatttcaac acgacgccgt actcgcctta cttcgcggac
     2821 tggctcgaag cgagcgcgct cacggactcg cggcgcggcc gcacgctgag ccctagctgg
     2881 cccacgatgt tgcagttggt cggcatcccc atcgatcatg ttgcagtgaa cgacggcttt
     2941 acgatcctct cgcatcgccg cctgccgaac ttcgagtcgg accacttcgg cgcgctcgtc
     3001 gaagtcgcgt tgcggcaggc ggagcaccca tgagcgcccc gccgaccgcg tgcgtgctca
     3061 tcatcggcaa cgagatcctg tccggcaaaa cgcaggacac gaacctgcag ttcttaggct
     3121 tcgagctcgc gaagctcggc attcggctcg ccgaagcgcg cgtcgtgcgc gacgagccgg
     3181 ccgcgatcat ccgccatttg aacgaagcgc gccgcgactt cacgtacgtg ttcacgaccg
     3241 ggggcatcgg tcccacgcac gacgacatca cggccgagtg cgtcgcgcag gcgttcggcg
     3301 tgccgctcgt gcggagcgag gaggcggtcg agcgcctgaa gcgcggcggc cgcccgctca
     3361 acgaagcgcg gctcaagatg gcgcgcgttc cgcgcggcgc gcagctcatc gagaatcccg
     3421 tcagcaacgc gccgggtttt cgcatcgaga acgtgttcgt gttcgccggc attccgagca
     3481 tcgcgcgcgc gatgttcacg agcgcgatcc cgatgctcgt gcacgctccg ccgattctgt
     3541 cggcgagcgt cgacgttcat ctgaaagaag gcgatttcgc cgaatcgctc gagcagatcc
     3601 agagccgtca tccaacggtc gagatcggca gctacccatt caaccgcgac ggccggttcg
     3661 gcgccacgct cgtcgcgcgc ggcacggatc gcgcgctgct cgcgaacgtc gtcgacgaga
     3721 tcgtggcggc gatgacgaag ctcggcggcg agactcacgt gctctgagcc gcgcgctttg
     3781 ccagcgcgcc cgagctcgcc gggcagaatt catcataatt ggccgtcgaa cggccacgat
     3841 tcgccgttcc cgtgtccgcg atcgcggcgc cgaatggcct ccaagggaat cctcggaggg
     3901 tcggcgatgc gcaagtggaa attcgagtgg cgagggaagc tgccgtcgag cgcgtacgag
     3961 gagctcgagg tctgggtctc ggtgctgctc atgctcgcgg cggcgctgct gctctacgcg
     4021 acgttgaagg cgcaggccgc gcgcgccgcc gaatttccgc gcgacatcgg cgcgccgacg
     4081 ctgttgttcc aggacggaag cggctcgtac tcggcggccg ccccgctcga aaccgatgtg
     4141 cggttgtcgg tcgccggcgt cgtcgcgcgc gtgcgcgtcg cgcagcgctt ccgcaacgtc
     4201 ggatcgacat acctcgaggc cgtctacgtg ctgccgctgc ccgacgacgc ggccgtcgac
     4261 cgcctctcga tgaagatcgg cgagcgcgtg atcgagggag agatccacga gcgtgccgcg
     4321 gccgagcgca tctacggccg ggcgcgcgcc gccgggcagc gcgcgagcct cgtgcggcag
     4381 acgagcgcca acctgttcac gacggccgtc gcgaacattg cgcccggcga gcacatcgac
     4441 atcacg
//
LOCUS       DASSNN010000109         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_22656, whole genome shotgun sequence.
ACCESSION   DASSNN010000109 DASSNN010000000
VERSION     DASSNN010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22656"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFL84_01970"
     CDS             complement(<1..241)
                     /locus_tag="VFL84_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HET7607415.1"
                     /translation="MPIAAILTTALTLFCIVHLFRTGRPMMWLFVILMVPVVGPLAYL
                     FVEVLSGFGQNPAARKAWSRARKAIDPNRGVREGSL"
     gene            352..981
                     /locus_tag="VFL84_01975"
     CDS             352..981
                     /locus_tag="VFL84_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET7607416.1"
                     /translation="MIDLYTWTTPNGRKVSIMLEELELPYRAIPVDINAQEQFKPEFL
                     ALSPNNKIPAIVDHETGIALMESGAILLYLADKTGRLRSSGKNGWQDVEWLMMQMGSV
                     GPMLGQAHHFLKFNRGKSEYAEKRYGDEAKRIYGVLNKRLGEAEYLAGEYSIADIATW
                     PWIARYEWQGIDFAQYPELKRWYLAIAARPAVRRGYHVPKEVSPIPLPT"
     gene            complement(956..1168)
                     /locus_tag="VFL84_01980"
     CDS             complement(956..1168)
                     /locus_tag="VFL84_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET7607417.1"
                     /translation="MNRTTGTSVEPKHRHFGWAGTSVGHAELAAQARGWIDAGARIVG
                     GCCGTTPEHIRALAALRAVMSAAESD"
     gene            complement(1221..2126)
                     /locus_tag="VFL84_01985"
     CDS             complement(1221..2126)
                     /locus_tag="VFL84_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET7607418.1"
                     /translation="MEQRWQTLLAHDGTLVLDGGTGSELRRRGVPLDAAVWSALAPLT
                     HYELLRAIHLDYIAAGADVVTTSTFATTRFVLEAAGLGGDFAAVNAKAVAAAREARDA
                     AGREVAIAGSISCLPPRFDVHAYPDETTERAAYLELAETLAEAGVDLLALEMLQETRH
                     APLACEAARAVGLPVWLGVSCRLGAGDTLVGFDFPLVTLAECLDALLPFMPAAVNVMH
                     SPVTAIEPALREVRARWPGPLGAYPEIGDGTPDVPHSVTPDGLAAHARGWIVSGARII
                     GGCCGTTPEHIRALAALRDATAAEG"
     gene            2186..>2700
                     /locus_tag="VFL84_01990"
     CDS             2186..>2700
                     /locus_tag="VFL84_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7607419.1"
                     /translation="MDAIANPYAPGAGTPPPELAGREELREQVRVAVERLRRGSPSKS
                     ILMVGLRGVGKTVLLDRMRDDSEASGIHTLRIEAPEGRSLPALIAPELRVALLRLSRN
                     EQAKAFGERALRALAGFARALKVKFHDIEVGVDFEPEPGLADNGDLEHDLAALLETVG
                     AAAQRAGTALVL"
BASE COUNT          464 a          899 c          926 g          411 t
ORIGIN      
        1 tcagcgagcc ttcgcgtacg ccgcgattcg ggtcgatggc cttgcgagcg cggctccacg
       61 ctttgcgcgc ggcaggattc tgaccgaagc ccgacagcac ctcgacgaac aggtacgcga
      121 gcggaccgac caccggcacc atgaggatca cgaacagcca catcatgggg cggcccgtgc
      181 ggaacaagtg gacgatgcag aagagcgtca gagcggtcgt cagtatggct gcgatcggca
      241 tacaacaaga ggcgatcagc gggtggcaac gtccatcatt ctcgaccatg ggattcgtgg
      301 caagcgggcc gccgtaaaat cgcttctctt ttgtgatcga atgctcaatt aatgatcgac
      361 ctctacacct ggacgacgcc gaacggccgc aaagtctcga tcatgctcga ggagctcgag
      421 ctgccgtacc gcgcgattcc cgtcgacatc aacgcgcagg aacaattcaa gccggagttc
      481 ttggcgctta gcccgaacaa caagattccc gccatcgtcg atcacgagac aggcatcgca
      541 ttgatggagt ccggcgcgat cttgctgtac ctcgccgata agaccgggcg cctgcgcagc
      601 tccggcaaga acggctggca ggacgtcgag tggctcatga tgcagatggg cagcgtcggg
      661 ccgatgctgg ggcaagcgca tcatttcttg aagttcaacc gcggcaagtc tgagtacgcc
      721 gagaagcgct acggcgacga agcgaagcgc atttacggcg tcttgaacaa acggctcggc
      781 gaagccgagt acctcgccgg cgagtactcg attgccgaca tcgcgacgtg gccgtggatc
      841 gcgcgctacg aatggcaggg catcgacttc gcgcaatatc ccgagctcaa gcgctggtat
      901 ctcgcgattg cggcgcggcc ggccgtgcgg cgcggctatc acgtgccgaa ggaagtcagt
      961 ccgattccgc tgccgacatg acggcgcgca gcgccgccag cgcgcggatg tgctccggag
     1021 tcgtgccgca gcagccgccg acgatgcgcg cgcccgcgtc gatccagccg cgcgcctgcg
     1081 ccgcgagctc cgcgtgccca accgaagtgc ctgcccaacc gaagtgccgg tgcttcggtt
     1141 caaccgaagt gccggtggtt cggttcaacc gaagtgccgg tggttcggtt taaccgaacc
     1201 gtaacaccgg cacgtcgcgc ctatccctct gccgccgtag cgtcgcgcaa tgccgcgagc
     1261 gcgcggatgt gctcgggcgt cgtgccgcag cagccgccga tgatccgtgc gccgcttacg
     1321 atccatccgc gcgcatgcgc ggcgaggcca tcgggcgtga ccgagtgcgg cacgtccggt
     1381 gtgccgtcgc cgatctcggg ataagcgccg agcggtcccg gccagcgtgc gcgcacctcg
     1441 cgaagcgccg gctcgatggc ggtcacggga ctgtgcatga cgttcacggc cgccggcatg
     1501 aacggcagca gcgcgtcgag gcactcggcg agcgtgacga gcgggaagtc gaatccgacc
     1561 agcgtatcgc cggcgccgag ccggcagctc acgccgagcc aaacgggcag ccccacggca
     1621 cgagccgctt cgcaggcgag cggcgcgtga cgcgtctcct gcagcatttc gagcgcgagt
     1681 agatcgacgc cggcttcggc cagcgtctcc gcgagctcga ggtacgcggc gcgctcggtg
     1741 gtctcgtcgg ggtatgcgtg cacgtcgaat cgcggcggca ggcacgaaat ggagccggcg
     1801 atcgcgacct cgcggccggc ggcatcgcga gcctcgcgcg ccgcggcgac ggctttcgcg
     1861 ttgacggccg cgaagtcgcc gcccaagccc gcggcctcga gcacaaaccg cgtcgtcgcg
     1921 aacgtgctcg tcgtgacgac gtcggcgccg gccgcgatgt agtccaagtg gatcgcgcgc
     1981 aagagctcgt agtgcgtcag cggcgccagc gcgctccaga cggccgcgtc gagcggcacg
     2041 ccgcggcggc gcagctccga gcccgttccg ccgtcgagca cgagcgttcc gtcgtgggcg
     2101 agcagcgttt gccagcgttg ttccatggct tatagcggct ttacggcagt ttatcgaatt
     2161 tagataaagt cactataatc gcggcatgga cgcgatcgcc aatccttacg ccccgggcgc
     2221 cgggacgccg ccgcccgagc tggcggggcg cgaggagctg cgcgagcaag tgcgcgtcgc
     2281 cgtcgagcgc ctgcggcgcg gcagcccgag caagagcatc ttaatggtcg gcctgcgcgg
     2341 cgtcggcaag accgtgctgc tcgatcgcat gcgcgacgat tccgaggctt cgggcatcca
     2401 tacgctgcgg atcgaagcgc cggaagggcg ctcgctgccg gcgctgatcg cgccggagct
     2461 gcgcgtggcg ctgctgcgcc tctcgcgcaa cgagcaagcc aaggcgttcg gcgagcgagc
     2521 gctgcgcgcg ctcgccggtt tcgctcgcgc gctgaaggtc aagttccacg acatcgaggt
     2581 cggcgtcgat ttcgagcccg agccagggct cgccgacaac ggcgatctcg agcacgacct
     2641 cgcggcgctg ctcgagacgg tcggcgcggc cgcgcaacgc gcgggcacgg cgctcgtgct
//
LOCUS       DASSNN010000110         2720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_528049, whole genome shotgun sequence.
ACCESSION   DASSNN010000110 DASSNN010000000
VERSION     DASSNN010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528049"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2569
                     /locus_tag="VFL84_01995"
     CDS             <1..2569
                     /locus_tag="VFL84_01995"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET7607420.1"
                     /translation="LRGHQIADLDPLGLWERPAPAVLSFDFLGLDESDLQREFSTGGL
                     AGTGHTRMKLQDILALLKHIYCGKLAAEFAHISRARERLWLREQFEACRVHWTFTKEE
                     QKTILDSLTRAEGIERYLNTRFVGQKRFSLEGSETLIPALDDLIQRAGEAGVQELVIG
                     MAHRGRLNVLVNVLGKSPSDLFSEFEGAYDLARLKGSGDVKYHKGYSADVRTPGGNVH
                     IALAFNPSHLEIVNPVVEGSVRARQDRRGDSLGEQVLPVLIHGDAAFAGQGVVAETFQ
                     LSQTRAFYTGGTVHLVVNNQIGFTTSDPHDSRSTTYCTDIAKMVEAPVFHVNGDDPEA
                     AVAAVRLALRYRQVFHKDVVIDLVCYRRLGHNEADEPSATQPIMYNAIREHPSTRSLY
                     ARKLIGEGVLTQADADKMADDYRTALDEGRNPNQAALGMIGNQYTVDWSRYTQASLND
                     SVSTALPEAELQRLAAIVNTVPPAHKLHPRVQRIMEDRRRMAAGELRCDWGFAETLAY
                     ASLLAADFHVRLVGQDTRRGTFFHRHAALIDQTTGDAYTPLAHVATDPGAFMVADSLL
                     SEEAVLGFEYGYSTTSPDTLVIWEGQFGDFVNGAQVVIDQFISSGEAKWGRLSGLTLF
                     LPHGYEGQGPEHSSARLERFLQMCAEHNMQVVVPSTPAQMFHLLRRQMLRHLRKPLVV
                     MTPKSLLRHRLSVSTMADLARGRFQPIIDEVEPLEDDAVTRVVFCSGKVYFDLAEHRR
                     NEGRMNVAIVRIEELYPFPIDEYARVVARYRNAKEIVWCQEEPQNQGAWYQIRHRLQD
                     PLGPEHELFYAGRPGAAAPASGIHALHVRQQQALVAAALTSSSKERAASLSPVGKRA"
BASE COUNT          479 a          921 c          873 g          447 t
ORIGIN      
        1 cctgcgcggc catcaaatcg ccgacctcga cccgctcgga ttgtgggagc gcccggcgcc
       61 ggccgtgctc tcgttcgatt tcttgggcct cgacgagtcg gatctgcagc gcgaattctc
      121 tacgggcggg ctcgcgggca ccggccatac gcgcatgaag ctccaggaca tcctggcgct
      181 cttgaagcac atctactgcg gcaagctcgc cgccgagttc gcgcacatct cgcgcgcgcg
      241 cgagcggctc tggttgcgcg agcagttcga ggcttgccgt gtccactgga cgttcacgaa
      301 ggaagagcag aagacgattc tcgatagcct cacgcgcgcc gaaggcatcg agcgttatct
      361 caacactcgc ttcgtcggcc agaagcgttt ttcgctcgaa ggcagcgaga cgctgattcc
      421 ggcgctcgac gatctgattc aacgcgccgg cgaagccggc gtgcaagagc tcgtgatcgg
      481 catggcgcat cgcgggcgct tgaacgtgct cgtcaacgtg ctcggcaagt cgccctcgga
      541 cttgttctcc gagttcgagg gcgcgtacga cctcgctcgg ctcaaaggct ccggcgacgt
      601 caagtatcac aaaggctatt cggccgacgt gcgcacgccc ggcggcaacg tgcacatcgc
      661 gctcgcgttc aatccgtcgc acctcgagat cgtgaacccc gtcgtcgaag gctcggtgcg
      721 cgcgcggcag gaccgccgcg gcgactcgct cggcgagcaa gtgctgccgg tgctgatcca
      781 tggcgacgcg gcgttcgccg gccaaggcgt cgtcgcggag acgttccagc tgtcgcagac
      841 gcgtgcgttc tacaccggag gcacggtcca tctcgtcgtc aacaatcaga tcggcttcac
      901 gacgagcgac ccgcacgaca gccgctcgac gacctattgc acggacatcg cgaagatggt
      961 cgaagcgccc gtgttccacg tgaacggcga cgaccccgaa gcggccgtcg ccgccgtgcg
     1021 gctcgcgctg cgctaccggc aggtgttcca caaggacgtc gtcatcgacc tcgtctgcta
     1081 tcgccggctc ggccacaacg aggccgacga gccgtcggcg acgcagccga tcatgtacaa
     1141 cgcgattcgc gagcatccga gcacgcgctc tttgtacgcg cgcaagctga tcggcgaggg
     1201 cgtcttgacg caggcggatg cggacaagat ggccgacgac taccgcaccg cgctcgacga
     1261 ggggcgcaac ccgaaccaag ccgcgctcgg catgatcggc aatcaataca cggtcgactg
     1321 gagccgctac acgcaagcct cgctgaacga ctccgtcagc acggccttgc ccgaagccga
     1381 gctgcagcgg ctcgccgcca tcgtcaacac cgtgccgccg gcccacaagc ttcacccgcg
     1441 cgtgcagcgc atcatggaag atcgaaggcg catggccgcc ggcgagctgc gctgcgattg
     1501 gggtttcgcc gagacgctcg cgtacgcgtc gctgctcgcc gcggacttcc acgtgcggct
     1561 cgtcggccaa gacacccggc gcggcacgtt cttccaccgc catgccgcgc tgatcgacca
     1621 gaccacgggc gacgcctata cgccgctcgc gcacgtcgcg accgatccgg gagcgttcat
     1681 ggtcgccgac tcgctgctgt cggaagaggc cgtgctcggc ttcgagtacg gctacagcac
     1741 gacgtcgccc gacacgctcg tcatctggga agggcagttc ggcgacttcg tcaacggggc
     1801 gcaagtcgtc attgaccagt tcatcagctc cggcgaagcg aagtggggcc gattgtcggg
     1861 gctcacgctg ttcctgccgc acggctacga aggtcaaggt cccgagcact cgtccgcgcg
     1921 cctcgagcgc ttcctgcaga tgtgcgcgga gcacaacatg caggtcgtcg tgccgtcgac
     1981 gccggcgcag atgtttcact tgctgcggcg gcagatgctg cggcatttgc gcaaaccgct
     2041 cgtcgtcatg acgccgaaga gcttgctgcg tcatcggctg tcggtgtcga ccatggccga
     2101 cctcgctcgc ggccgtttcc aaccgatcat cgacgaggtc gagccgctcg aggacgacgc
     2161 cgtcacgcgc gtcgtgttct gcagcggcaa agtttatttc gacctggccg agcaccgccg
     2221 caacgaaggc agaatgaatg tcgcgatcgt gcgcatcgag gagctctacc cgttcccgat
     2281 cgacgaatac gcgcgcgtcg tcgcgcgcta tcgcaacgcg aaggaaatcg tgtggtgcca
     2341 ggaagagccg cagaatcagg gcgcctggta tcagattcgt caccgcctgc aggatccgct
     2401 cgggccggag cacgagctct tctatgcggg caggcccgga gccgccgcgc cggcatccgg
     2461 tattcacgcg ctgcacgtgc gtcagcagca agcgttggtc gccgccgcgt tgacgtcgtc
     2521 gtcgaaagaa cgcgccgcga gtctttcgcc ggttggtaaa cgcgcctagc atcgaagcgg
     2581 aaccggcgct tgaggtaagg acatcccgtg agcatcgagg tcaaggttcc gcaactgccc
     2641 gaatcggtcg ccgacgccac gctggtcacc tggcacaaga aggccggcga cgccgtgcgc
     2701 cgcgaggaga acctcgtcga
//
LOCUS       DASSNN010000111         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_528509, whole genome shotgun sequence.
ACCESSION   DASSNN010000111 DASSNN010000000
VERSION     DASSNN010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528509"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2100
                     /locus_tag="VFL84_02000"
     CDS             <1..>2100
                     /locus_tag="VFL84_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7607421.1"
                     /translation="VAGIEGILKLESDSEDERSQVRIEFDINRDVDAAANDVRDRVAR
                     VMRGLPPEADPPQIVKADANADSVVTLAFSSDTISMLELTDYAERNIVDRMSTVPGVA
                     SVGIVGGRRYSMRIWIDRQELAARQLTVTDIEDALRRENIELPAGRIESRTREFSLRT
                     MVGLESEQDFRNLVIERGADGHLVRLGEVADVRLAAENERSYSRLNGVPGLMMQVQAQ
                     SKGNTLDIARGVREEVARIQASLPAGMTLNVSVDNAVPIEAALHEVLIAVGFALASVL
                     AVIYLFLGNIRATLIPAVTIPVSIVASLTVMYSLGYSVNVLTLLGLVLAIGLVVDDAI
                     VVLENIHRRHELGEPTLVAAITGSREIGFAVIATTLTLVSVFVPISFLPGDLGRLFRE
                     FGFTLAAAVAFSALIALTLTPMLASRLPEQERRNRFAAFIDKFFKQLARFYERCLRGA
                     LRRPLAVVAVLLALIGVGIVTFLSVPSEFTPQADNGRAFIAIESPEGSSFDYIDRYAR
                     QLEAIALKTRDGGEVERVLVRVPGQGGQDLRTGDINTARAFLIFSDWDERQHTADEIM
                     QPLLAEARKLPGVRVSMGRPGGLGRGGLQSPVVAVIGGPDYERLAVWSDQMLQLAQSN
                     PGLVNVQSNYKERKPQIRVSIDRDRAADLGISLESVGRTLETVLGSRIVTTYIDRGRE
                     YNVILQGRDDIRETTTDL"
BASE COUNT          320 a          749 c          685 g          346 t
ORIGIN      
        1 gtcgccggca tcgaaggcat cttgaagctc gagtccgaca gcgaggacga gcggtcgcaa
       61 gtccgcatcg agttcgacat caaccgcgac gtcgacgccg ccgccaacga cgtgcgcgac
      121 cgcgtcgcgc gcgtcatgcg aggcctgccg ccggaagccg acccgccgca gatcgtgaag
      181 gccgacgcga acgcagactc cgtcgtcacg ctcgcgttct cgtccgatac gatctcgatg
      241 ctcgagctca ccgactacgc cgagcgcaac atcgtcgacc gcatgtcgac ggtgcccggc
      301 gtcgctagcg tcggcatcgt cggcggacgc cgctattcga tgcgcatttg gatcgaccgg
      361 caggagctcg cggcccggca gctcacggtc accgacatcg aggacgcgct gcgccgcgag
      421 aacatcgagc ttccagccgg ccgcatcgag tcgcgcacgc gcgagttttc gctgcgcacg
      481 atggtcggcc tcgagagcga gcaggatttc cgcaacctcg tcatcgagcg cggggccgac
      541 gggcacctcg tgcggctcgg cgaggtcgcc gacgtacggc tcgccgccga gaacgagcgt
      601 tcctacagcc ggctgaacgg cgtgcccggt ctcatgatgc aggtccaggc gcagtcgaag
      661 ggcaacacgc tcgacatcgc gcgcggcgtg cgcgaggaag tcgcacggat ccaggcgagc
      721 ctgcccgcgg gcatgacgct gaacgtcagc gtcgacaacg cggtgccgat cgaggccgcg
      781 ttgcacgagg tgctgatcgc cgtcggattc gcgctggcgt cggtgctcgc agtcatctat
      841 ctgttcctcg gcaacattcg cgcgacgctt attcccgccg tcacgatccc cgtgtcgatc
      901 gtcgcgtcgt tgaccgtcat gtactcgctc gggtactccg tcaacgtgct gacgctgctc
      961 ggcctcgtgc tcgcgatcgg cctcgtcgtc gacgacgcga tcgtcgtgct cgagaacatt
     1021 caccgccgtc acgagctcgg cgagccgacg ctcgtcgccg cgatcacggg ctcgcgcgag
     1081 atcggcttcg ccgtcatcgc gacgaccttg acgctcgtct ccgtgttcgt gccgatctcg
     1141 ttcctgccgg gcgacctcgg ccgacttttc cgcgagttcg gcttcacgct cgcggccgcc
     1201 gtcgcgttct cggcgctgat cgcactgacc ctgacgccga tgctcgcctc gcgcctgccc
     1261 gagcaagagc gccgcaaccg cttcgctgcc ttcatcgaca agttcttcaa gcagctcgcg
     1321 cgtttctacg agcggtgcct gcgcggcgcg ctgcgccgtc cgctcgccgt cgtggccgtg
     1381 ctcctcgcgt tgatcggcgt cggcatcgtc acgttcttgt cggttccatc cgagttcacg
     1441 ccgcaagccg acaacggccg cgcgttcatc gcgatcgagt cgcccgaggg ctcgagcttc
     1501 gactacatcg accgctacgc gcgtcagctc gaggcgatcg cgctgaagac gagggacggc
     1561 ggcgaggtcg agcgcgtgct cgtgcgcgtg ccgggccaag gcggccagga tctgcgcacc
     1621 ggcgacatca acacggcccg cgcgttcctg atcttttccg actgggatga gcgccagcac
     1681 acggccgacg agatcatgca gccgctgctc gccgaggcgc gcaagctgcc cggcgttcgg
     1741 gtctcgatgg ggcggcccgg cggcctcggc cgcggcggcc tccagtcgcc cgtcgtcgcc
     1801 gtgatcggcg ggcccgacta cgagcgcctc gccgtctggt cggatcagat gctgcagctc
     1861 gcgcaatcga accccgggct cgtgaacgtg cagagcaatt acaaggagcg caagccgcag
     1921 atccgcgtct cgatcgaccg cgaccgcgcc gcggacttgg gtatatcgct cgaaagcgtc
     1981 gggcgcacgc tcgagaccgt cctcggctcg cgcatcgtga cgacgtacat cgatcgcggc
     2041 cgcgagtaca acgtgatcct gcagggccgc gacgacatcc gcgagacgac gaccgacctg
//
LOCUS       DASSNN010000112         4491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1410828, whole genome shotgun sequence.
ACCESSION   DASSNN010000112 DASSNN010000000
VERSION     DASSNN010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410828"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..2080
                     /locus_tag="VFL84_02005"
     CDS             311..2080
                     /locus_tag="VFL84_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7607422.1"
                     /translation="MPRSHIAKHCRTVRAAQRIFLVALAALAAGESRVVFAQTPAAQA
                     FRPVTDAMLEDPPAADWLSFRRTLNAWGYSPLDQINGGNVQGLHEVWSAPVAGSEVTP
                     LVHDGVMYLPLGGDGFVALDAATGKELWRFARELPNGRAPGGTKRNIAIYQELLISTA
                     GDGSVYAVDARTGKQAWEVKITGPANTSSGPIIANGKVISGRACAPDRGPEGCVMVAN
                     DARTGKELWRTWTIAKPGEPGDETWGSVPWEKRNQVGTWMPPSYDPELNLVYFGTSVT
                     GPTPKYLMAGNDKTYLYHTSTLALDADTGKIVWYYQHIVDHWDFDHTFDRILVDTEVA
                     PAAGEVAWINPNVRAGEVRKVLTGIPGKTGIVYTLDRRTGEFLWAEPTVKQTVVSSID
                     GATGKVHMNPDTVFTRDDQAIDLCPAFTGGKNWMPGAYSPQTGLMYMPLENLCSVVTS
                     AGPKTGAGQLGMKIDYTAKLAPGETNVGRVQAISVKTGATAWSYEQRAGMMAVVATGG
                     ALVFAGDVAGVFRALDAKTGEVRWQTQLPGAISGIPISYGAGGKQFVAVATGSSPEAG
                     GLARMTPEIRVQNERVLHVFALN"
     gene            2147..>4491
                     /locus_tag="VFL84_02010"
     CDS             2147..>4491
                     /locus_tag="VFL84_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607423.1"
                     /translation="MTTIRQDIRYGIRTLRDRPAFTLVATLSLALGIGANATIFSIIN
                     ATLLAPLGLAHEDRLVALTTHPLESPNNRGSAAYREFEAWQDAKSFEAVGAIWTVPEI
                     LGGASDGTSAAEDVLAIRGGPRLFEVLDVKPQLGRLMAPDEDQVESWAPVALISDRFW
                     ERRFERDPQVVGKTIRLDGVVTTIIGVMPPGIERSIFEPDADLWVPSQTNAAQVISAA
                     GFLTVFARLAPGATVEQARAELRTMAQRFAEQYPDSNKNRGFGVMPIHDLFYGGAKEP
                     LLILQGAVLFVLLIACANVAGLLLARAAARQTEIAIRSAVGAARLRLIRQVMTESVVL
                     AVLGGILGVVFAWIGLRVFVAAAPPDIPNLDQMTVNPEVLAFATLIVVVTALAFGMVP
                     ALQGTRPDLTTLLNDSARGSSAGAGRQRLRLALVAGQVGVAMILLISAGLLINSFLKL
                     RGNELGADPTGVLTFQVRFGQGETITFTGQQVGGVGLWNVNPLVGVTLERIYEELKAV
                     PGVEAVSVGNPLPFQGAPFRNFLIDGRAASGDGARQNGAYFAVMPGYFEALKIGVRRG
                     RALDDNDNAAGRRVVVINEALATQYFKERDPIGSYITLDFVPGEQPREVVGVVQNVLL
                     NQYADSFTPTMYVPFVQQTDTWLGPQWAQRADASFVVRGRGDPMDLVPVVRSVVARVD
                     ADRPVTRIRTIEQYLVQQRQGDALWVGLLGTFGIIAGVLAVTGIYGVISYAVAQRTHE
                     IGIRVALGASARTIVALIMRQAVVVVAAGLVVGVIGSLVLTR"
BASE COUNT          715 a         1515 c         1540 g          721 t
ORIGIN      
        1 ccgtaacgat cgcactgtcc aagcggagct tgtcgcgtct cggcaactct cgacgttgcg
       61 cgatcaatgc cttgcgcgtg accttgcgcc actcttggat tcccttccaa tcatccggca
      121 tgtattcgtt ccacccatga acacgacgac cttacgcgcg gagccgcgcc ccgagcaaac
      181 aaaaaaaacc gccgtcggca atcgaccata tcgattcaaa ttcgtccgca gccgacgatc
      241 tcgttgccgt tcgcagcgtc gtgaactcac ggtatagtcc catgcgccca ctggtcgagc
      301 gcggggcccc atgccacgaa gtcacatcgc caagcattgc cggacggtcc gagccgcgca
      361 gcgaatcttc ttggtcgcgc tcgccgccct agcggcaggc gagtcgcggg tcgtcttcgc
      421 ccaaacgccg gcggctcagg cgttccgccc cgtcaccgac gccatgctcg aggacccgcc
      481 ggccgccgat tggctgtctt tccgccgcac gttgaacgcg tggggttaca gcccgctcga
      541 ccagatcaac ggcggcaacg tgcaggggct gcacgaggta tggtccgcgc cggtcgcggg
      601 ctccgaggtc acgccgctcg tgcacgacgg cgtcatgtac ctgccgctcg gcggcgacgg
      661 gttcgtcgcg ctcgacgcgg ccacgggtaa ggagctctgg cggttcgcgc gcgagctgcc
      721 gaacggccgt gcaccgggcg gcaccaagcg caacatcgcg atctatcaag agctcttgat
      781 ctcgaccgcc ggcgacggct cggtctacgc cgtcgacgcg cgcaccggca agcaggcctg
      841 ggaggtcaag atcacggggc cggccaacac ctcgagcggg cccatcatcg cgaacggcaa
      901 agtaatctcg ggccgcgcgt gcgcgcccga ccgcgggccg gagggctgcg tcatggtcgc
      961 caacgacgcg cgcacgggca aggagctgtg gcgcacgtgg acgattgcga agcccggcga
     1021 gccgggcgac gagacgtggg gcagcgtgcc gtgggagaag cgcaaccaag tcggcacgtg
     1081 gatgccgccg agctacgacc cggagctgaa tctcgtgtac ttcggcactt cggtgacggg
     1141 cccgacgccg aagtacttga tggccggcaa cgacaagacc tacctctatc acacctcgac
     1201 gctggctctc gatgccgata ccggcaagat cgtctggtac taccagcaca tcgtcgacca
     1261 ctgggacttc gaccacacct tcgatcgcat cctcgtcgac accgaggtcg cgccggccgc
     1321 gggcgaagtg gcgtggatca acccgaacgt ccgagccggc gaagtgcgca aggtgttgac
     1381 gggcatcccg ggcaagaccg gcatcgtcta cacgctcgat cgacgcaccg gcgaattcct
     1441 gtgggccgag ccgaccgtga agcagaccgt cgtgtcgtcg atcgacggcg cgacgggcaa
     1501 ggtccacatg aaccctgaca ccgtcttcac gcgcgacgac caagccatcg acctgtgccc
     1561 ggcctttacc ggcggcaaga actggatgcc gggcgcgtac agcccgcaga cggggctcat
     1621 gtacatgccg ctcgagaatc tgtgctcggt cgtcacgagc gcggggccga agacgggcgc
     1681 gggccagctc ggaatgaaga tcgactacac ggcgaagctc gcgcccggtg agacgaacgt
     1741 cggccgcgtt caggcgatct ccgtcaagac gggcgcgacc gcgtggagct acgagcaacg
     1801 cgccggcatg atggccgtcg tcgcgacggg cggcgcgctc gtgttcgcgg gcgacgtcgc
     1861 cggcgtgttc cgcgcgctcg acgcgaagac cggcgaggtg cgttggcaga cgcagctccc
     1921 cggggcgatc tcgggcattc cgatcagcta cggcgcgggc ggtaagcagt tcgtcgccgt
     1981 cgcgacgggg tcgtcgcccg aggcaggcgg cctcgcgcgc atgacgcccg agattcgggt
     2041 ccagaacgag cgcgtgctgc acgtctttgc actgaactga tcgcgtaacc gttgaaggcg
     2101 cgggcggctc tattagagtc tccgccactc tagagggcgc gttgcgatga cgacgattcg
     2161 ccaagacatc cgctacggca ttcggactct tcgcgaccgg cccgcgttca cgctcgtggc
     2221 cacgctgtcg ctcgcgctcg gcatcggcgc caacgcgacg atcttcagca tcatcaacgc
     2281 cacgctgctc gcgccgctcg gtctcgcgca cgaagatcgg ctcgtcgcgc tcacgacgca
     2341 tccgctcgag tctccgaaca atcgcggcag cgccgcctac cgcgaattcg aggcctggca
     2401 ggacgcgaag agcttcgagg ccgtcggcgc gatctggacg gtgcccgaga tcttgggcgg
     2461 cgcgagcgac ggcacttcgg ccgcggagga cgtgctcgcg atccgcggcg ggccgcgctt
     2521 gttcgaggtt ctcgacgtca agccgcagct cggccgtctc atggcgcccg acgaagatca
     2581 ggtcgagagc tgggcgccgg tcgcgctgat cagcgatcgc ttctgggagc ggcgcttcga
     2641 gcgcgacccg caagtcgtcg gcaaaaccat tcggctcgac ggcgtcgtga cgacgatcat
     2701 cggcgtgatg ccgcccggca tcgagcgcag catcttcgag cccgacgcgg acttgtgggt
     2761 gccgtcgcaa accaatgcgg cgcaggtcat cagcgcggcg ggttttctca cggtgttcgc
     2821 gcggctcgcg ccgggagcga cggtcgagca ggcgcgcgca gagctgcgca cgatggcgca
     2881 gcggttcgcc gagcagtatc cggactcgaa caagaaccgc ggcttcggcg tcatgccgat
     2941 ccacgatctt ttctacggcg gggcgaagga gccgctgctg atcctgcagg gcgccgtgct
     3001 gttcgtgctg ctgatcgcgt gcgcgaacgt cgcgggtctc ttgctcgcgc gcgcggcggc
     3061 gcgccagacc gagatcgcga tccgcagcgc cgtgggcgcg gcgcgcttgc gcttgattcg
     3121 ccaggtcatg accgagagcg tcgtgctggc ggtgctcggc ggcattctcg gcgtcgtgtt
     3181 cgcctggatc ggcttgcgcg tgttcgtcgc cgctgcgccg ccggacatcc cgaacctcga
     3241 ccagatgacg gtcaaccccg aagtgctcgc gttcgcgacg ctgatcgtcg tcgtcacggc
     3301 gctcgcgttc ggcatggtgc ccgcgctcca gggcacgcgc cccgacctga cgacgctgct
     3361 caacgattcc gcgcgcggct cgagcgcggg cgccggccgg caacggctcc ggctcgcgct
     3421 cgtcgcgggt caggtcggcg tcgcgatgat tctcttgatc agcgcgggcc tcctcatcaa
     3481 cagcttcctg aagctgcgcg gcaacgagct cggcgccgac ccgacgggcg tcctgacgtt
     3541 ccaagtgcgc ttcggccagg gcgagacgat cacgttcacg ggccagcagg tcggaggcgt
     3601 cgggctctgg aacgtcaacc cactcgtcgg cgtgacgctc gagcgcattt acgaagagct
     3661 gaaggcggtc cccggcgtcg aggccgtctc ggtcggcaat ccgctgccgt tccagggggc
     3721 gccgtttcgc aacttcctga tcgacgggcg ggcggcgagc ggcgacggcg cgcggcagaa
     3781 cggcgcgtac ttcgccgtga tgcccgggta tttcgaagcg ttgaagatcg gcgttcgccg
     3841 cggccgtgcg ctcgacgaca acgacaacgc ggctggcagg cgagtcgtcg tcatcaacga
     3901 ggcgctggcg acgcagtatt tcaaggagcg cgatccgatc ggcagctaca tcacgctcga
     3961 cttcgtgccg ggcgagcagc cgcgcgaggt cgtcggcgtc gtgcagaacg tgctgctgaa
     4021 tcagtacgcg gactcgttca cgccgacgat gtacgtgccg ttcgtgcagc agaccgacac
     4081 ctggctaggg ccgcagtggg cgcagcgcgc ggacgcgtcg ttcgtcgtgc gcggccgcgg
     4141 cgatccgatg gatctcgtgc cggtcgtgcg cagcgtcgtc gcgcgtgtcg atgcggaccg
     4201 gccggtcacg cgaattcgca cgatcgagca gtacttggtg cagcagcggc aaggcgacgc
     4261 gctctgggtg ggattgctcg gcacgttcgg catcatcgcc ggcgtgctcg ccgtgaccgg
     4321 catctacggc gtcatcagct acgcggtggc gcagcgcacg cacgagatcg gtattcgcgt
     4381 agcgctcggc gccagcgcgc gaacgatcgt cgcgttgatc atgcgccagg ccgtcgtcgt
     4441 cgtggcggcg ggtctcgtcg tgggcgtcat cggctcgctc gtgctgacgc g
//
LOCUS       DASSNN010000113         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1651051, whole genome shotgun sequence.
ACCESSION   DASSNN010000113 DASSNN010000000
VERSION     DASSNN010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651051"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFL84_02015"
     CDS             complement(<1..309)
                     /locus_tag="VFL84_02015"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HET7607424.1"
                     /translation="MTEVAAAEALILAHMPKFPARQEPLSTCGGRVLAEDIHAERDQP
                     PFDRVTMDGIAVAFRDFASGIRSFEVVGTQAAGAEPLTVATAQCVEVMTGAVLPRGAD
                     T"
     gene            393..908
                     /locus_tag="VFL84_02020"
     CDS             393..908
                     /locus_tag="VFL84_02020"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7607425.1"
                     /translation="MVRIAFCGVASCLVLGPVLAQRPAIPELSQEQPDSRTVLGVVNE
                     YLAAGAEAIRLKEYDEGIRLTKLGLERPTSPRDRAAALSNLCAAHAAKNEPDAAIGYC
                     TESLTVNDANWRAYSNRAYAYYLKKMFEEAEADLDVALSINPNAKQMPQIRGMMNERR
                     LRGRVTMEEHQ"
     gene            911..1612
                     /locus_tag="VFL84_02025"
     CDS             911..1612
                     /locus_tag="VFL84_02025"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET7607426.1"
                     /translation="MLRQLTAAALIACFACASGTASAARGTLDHIGTWSCVVWGHPDF
                     GDERMLFNFAPQGVARLARLQSETTPAWSGLTPWVEENRELRFGDPRTGRQYTADLRR
                     DNLGGSWRTLTQTGGWWCVQSDIEAVPETNEARAAALPPVLPLVTATPRYPIEAIRAA
                     KQGRVVTCFLVDSEGVIVDPQIIELSDEMFREPILTALRRSRYERRDSDMLRPSCRSY
                     TFTLDRLAQHEIEIE"
     gene            complement(1613..>2429)
                     /locus_tag="VFL84_02030"
     CDS             complement(1613..>2429)
                     /locus_tag="VFL84_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7607427.1"
                     /translation="NRETLATLPAGGDLGRERYQAATALLRERAPAYDAYDKGREALA
                     DDRPAEAERAAQQALRLLPEEANIHALLGDIDMHERRYEEAARHYSDAIARNDGFFYY
                     HLQNGLALERLRDWERARAELEESVRLLPTADAVYALGTLAERRGDVRTALEYYAQAA
                     QSNSPAGQAAEEAAVRLDLPNNPGNYIQARGGLDNSGQLIVEVANPTRVAITDVIVAV
                     RYADADGRVREVARRVNELPPGQATRFATGLGPFTSAQQFQVGVTGARVARND"
BASE COUNT          376 a          809 c          833 g          411 t
ORIGIN      
        1 cgtgtctgcg cctcgcggca gcacggcgcc cgtcatgacc tcgacgcact gcgctgttgc
       61 gacggtgagc ggctccgcgc ccgccgcttg cgtgccgacg acctcgaacg agcgaatgcc
      121 ggaggcgaag tcgcggaacg cgacggcgat gccgtccatc gtcacgcggt cgaacggcgg
      181 ctgatcgcgc tcggcgtgga tgtcttcggc gagcacgcgg ccgccgcacg tcgacaaagg
      241 ctcttgccga gcgggaaact tcggcatgtg agccagaatc aaggcctcgg ccgcagccac
      301 ttctgtcata aggttgaaaa gcctttcggt cggattgcgc gcctgttcgg cctgccgccg
      361 ccgggactat actaatccgg gaggaggcaa gcatggtccg catcgcgttc tgcggcgtgg
      421 cgagttgtct cgtgctcggt ccagtgctcg cccaacgccc cgccattccg gagctgtcgc
      481 aagaacagcc agacagccga accgtgctcg gagtcgtgaa cgagtatctc gcggccggcg
      541 cggaagcgat tcgtctcaag gaatacgacg aaggcattcg gctcacgaag ctcggcctcg
      601 agcgcccgac gtcgccgcgc gaccgtgctg cggcgttgtc gaatctctgt gcggctcatg
      661 ccgccaagaa cgagcccgac gccgcaatcg gttactgcac cgagtcgctt acagtcaacg
      721 acgcgaactg gcgcgcatac agcaaccgcg catacgcgta ctacttgaag aagatgttcg
      781 aagaagccga ggccgatctc gacgtagcgt tgtcgatcaa tccgaacgcg aagcagatgc
      841 cgcagatccg cggcatgatg aacgagcgtc ggctgcgggg acgcgtgacg atggaggagc
      901 accagtagtc gtgcttcggc agctcaccgc ggcggcgctc atcgcgtgtt tcgcttgcgc
      961 gagcgggacc gcgagcgctg cgcgcggcac gctcgatcat atcggcacgt ggagctgcgt
     1021 cgtctggggt caccccgact tcggcgacga gcgaatgctg ttcaacttcg cgccacaagg
     1081 cgtcgcgcga ttggcgcgcc ttcagtccga gaccacgccg gcgtggagcg ggctcacgcc
     1141 gtgggtcgag gagaatcgcg agttgcgctt cggcgatccg cgcaccggcc ggcagtacac
     1201 ggcggacttg cgccgcgaca atctcggcgg cagctggcgg acgctgacgc agacgggcgg
     1261 ttggtggtgt gtgcaatcgg acatcgaggc cgtgccggag acgaacgagg cgcgcgcggc
     1321 ggctttgccg cccgtgttgc cgctcgtcac cgccacgccc cgctatccga tcgaggcgat
     1381 tcgcgcggcc aagcagggac gcgtcgtcac ctgttttctc gtcgattcgg agggcgtcat
     1441 cgtcgacccg cagatcatcg agctttccga cgagatgttt cgcgagccca tcctaacggc
     1501 gctacgccgg tcgcggtatg agcgccgcga ctccgacatg ttgcggccga gttgccgcag
     1561 ctacacgttc acgctcgacc ggctggcgca gcacgagatc gagatcgaat agctagtcgt
     1621 tgcgcgccac gcgcgcgccc gtcactccga cctgaaactg ttgggccgac gtgaacggcc
     1681 caaggccggt cgcaaaccgc gtggcctgcc cgggcggcaa ctcgttgacg cgccgggcca
     1741 cttcgcgaac gcggccatcg gcgtcggcgt agcgcaccgc gacgatcacg tctgtgatcg
     1801 ccacgcgcgt tggattcgcg acttcgacga tgagctgccc cgagttgtcg aggccgccgc
     1861 gcgcttgaat gtagttgccg gggttgttcg gcaggtcgag ccgaaccgcc gcttcctcgg
     1921 ctgcttggcc cgcggggctg ttcgactgcg ccgcttgcgc gtagtactcg agcgccgttc
     1981 ggacgtcgcc gcgccgttcg gccagcgtgc cgagcgcgta gacggcatcg gccgtcggca
     2041 ggaggcgcac gctctcctcg agctccgcgc gcgcccgttc ccagtctcgc agccgctcaa
     2101 gcgcgagccc gttttgcaag tggtagtaga agaagccgtc gttgcgcgcg atcgcgtcgc
     2161 tgtagtggcg agccgcttct tcatagcgcc gctcgtgcat gtcgatgtcg ccgagcaagg
     2221 cgtggatgtt cgcttcctcg ggcagcaagc gcagcgcttg ttgcgccgcg cgctccgctt
     2281 cggccggccg atcgtcggcg agcgcttcgc ggcccttgtc gtaggcgtcg taggcgggcg
     2341 cgcgctcgcg cagcagcgcc gtcgcggcct gatagcgctc gcggccgagg tcgccgccgg
     2401 cgggtagcgt cgccaaggtc tcgcggttc
//
LOCUS       DASSNN010000114         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1159202, whole genome shotgun sequence.
ACCESSION   DASSNN010000114 DASSNN010000000
VERSION     DASSNN010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159202"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2377)
                     /locus_tag="VFL84_02035"
     CDS             complement(<1..2377)
                     /locus_tag="VFL84_02035"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET7607428.1"
                     /translation="MSAVSLRRCVVVVVLPMLCAAPLVSCTQSSERLAAEHGATIEKY
                     CTDCHSAAEREADLVLENPDLLNPEAQRAQWEKVIHKLRAGLMPPPGEPRPDDEAVAG
                     MVSYLETTLDASEPEPAGAPVRRLTRAAYGNAVRDLLGFPVDVETLLPADITSDGFDN
                     VSDTLKTSPLLLERYLTVGLRLAALAVGDTKLSPRGTEYRPRLDLSQNDWIEGLPFGT
                     RGGLVVEHYFPTDAEYEIRPELWRATGSTVRGVEGFKTPFELQILLDGAVVHSAEFGG
                     VDDDALSNRDIGSAVMQVAARLPVRLAVSAGLHRVGVTFVAKSFALEQKLLDPDRADL
                     PIGNDGYGWPIVTRVLVGGPYGSTGPGDTPTRRAIFTCRPSASMTVTACADEILGRLA
                     LRAYGRPLDERDRQTLRELYVSGSDNGRDFEAGIELALARILSGPEFLLYHGLTEEAA
                     VSSSANEDDGAMLASRLALFLWNSIPDDALRDAGIAGRLHEPRVLEGEVQRMLADERA
                     GTLVTDFAEQWLQLRLVNAKSPDQRTFPTFDDNLRRDMLRETELFLRSVLLGDASVLE
                     LLRADYTFLNERLAEHYGIDGVFGDAFRRVTLQEPNRRGLLGQGSILFQSSVATRTSP
                     VFRGKWIMTNFFNAPSPPPPANVPALEASIADSTPHSVRERLEQHRKDPVCAGCHAII
                     DPPGLALENFDAIGRWRDADAGEAIDASTTLPGGIAVSGPRGLREAILSRPELFVSTF
                     TEKLMVYALGRRLEAEDMPTVRRIVREAASDDYKFSALVLGIVRSPQFQQKA"
     gene            complement(2374..3873)
                     /locus_tag="VFL84_02040"
     CDS             complement(2374..3873)
                     /locus_tag="VFL84_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7607429.1"
                     /translation="MTTLSKIGAFASALLISLGAWADSELDAKLLDAAQKANATEVRA
                     LLAAGADANARAADGSTPLVYAAHFGDTQSVRALLGAKADPNLANRYGVAPMHEAALR
                     ADADLLHTLLEAGASVDLALPQGETPLMLASRTSGIAAVRLLIERGANVDVVEQWQGQ
                     TPLMYAAAHDRGEVAAALIAAGADVNARTPINDLPERESRVRYYVEIPVAGFTPVMFA
                     ARSGANSALRSLIAGKADLDAKTPEGFTPIVIALDNLHFDTATVLVEGGASADDGGLL
                     GVVEARNRVNYVGEYQVPTGPELSLDLLALMLSKGADLMGRMPQPLLDRDARFVPPPP
                     KITDVALIRAARSADVGAMRVLIDGGADPSMRDDKRGGITPLVAVMTGPELPSLIEAD
                     RRPLEDDAIAALDFLLSRGADLTMGNDIGSTALHVAALRAYPGVIKHLAEKGADLNIG
                     DRDGFTPLDYAVGRLPPRLRTRPPADNPAAAALMELGARGKEEQRTAAR"
BASE COUNT          605 a         1364 c         1371 g          591 t
ORIGIN      
        1 gcgccttctg ctgaaactgc gggctacgca caatgccgag cacgagggcg ctgaacttgt
       61 agtcgtcgct cgcggcttcg cgcacgatgc ggcgcacggt cggcatgtcc tcggcctcga
      121 ggcggcgccc gagcgcgtag accatgagct tctccgtgaa cgtcgaaacg aaaagctctg
      181 ggcggctcaa gattgcctcg cgcaaaccgc gagggccgga cacggcgatg ccgcccggca
      241 acgtcgtgct cgcgtcgatg gcctcacccg cgtccgcgtc gcgccagcgg ccgatcgcat
      301 cgaaattctc gagcgcgagc cccggcggat cgatgatcgc gtgacagccc gcgcagaccg
      361 gatccttgcg atgctgctcg aggcgctcgc gcacgctgtg cggagtgctg tccgcgatgc
      421 tggcttcgag cgcgggcacg tttgccggcg gaggcggcga cggcgcgttg aagaaattcg
      481 tcatgatcca cttgccgcgg aacaccggcg acgtgcgcgt cgcgacggag ctctggaaca
      541 gaatgctgcc ctgcccgagc agcccgcggc gattcggctc ctgcaacgtg acgcggcgaa
      601 acgcgtcgcc gaacacgccg tcgattccgt agtgctccgc gagccgttcg ttcaagaacg
      661 tatagtcggc gcggagcagc tcgagcacgc tcgcatcgcc gagcagcacg gagcgcagaa
      721 agagctccgt ctcgcgcagc atgtcgcggc gcaggttgtc gtcgaaggtc gggaacgtgc
      781 gctgatcggg cgacttcgcg ttgacgagcc gcagctgcag ccattgctcg gcgaaatcgg
      841 tgacgagcgt gccggcgcgc tcgtcggcca gcattctctg cacttcgcct tcgagcactc
      901 gcggctcatg caagcggccc gcgatgccgg catcgcgcag cgcgtcgtcg gggatgctat
      961 tccagaggaa cagcgcgagc cgcgacgcga gcatcgcgcc gtcgtcctcg ttggcgctcg
     1021 aggagaccgc ggcttcctcg gtcagcccgt ggtacagcag aaactcgggc ccgctcaaga
     1081 tgcgcgcgag cgcgagctcg atgccggcct cgaagtcgcg gccgttgtcg ctgccgctca
     1141 cgtagagctc gcgcagcgtt tgccgatctc gctcgtcgag cggccggcca taggcgcgca
     1201 gcgcgaggcg gcccaagatc tcgtcggcgc acgccgtgac ggtcatggac gcgctcggcc
     1261 gacaagtgaa aatcgctcgc cgcgtcggcg tgtcgccggg ccccgtcgag ccgtacggcc
     1321 cgccgacgag cacgcgcgtc acgatcggcc agccgtagcc gtcgttgccg atcggcaagt
     1381 cggcgcgatc cggatcgagc agcttctgct cgagcgcgaa gctcttcgcg acgaacgtga
     1441 cgccgacgcg atgcagcccg gcgctgacgg ccaagcgcac gggaagccgc gccgcgactt
     1501 gcatcaccgc gctgccgatg tcgcggttcg agagcgcgtc gtcgtcgacg ccgccgaact
     1561 cggcggaatg cacgacggcg ccgtcgagca ggatctgcag ctcgaacggc gtcttgaacc
     1621 cttcgacgcc gcgcaccgtg ctgcccgtcg cgcgccacag ctccgggcgg atctcgtact
     1681 cggcgtccgt cgggaaatag tgctcgacga cgagcccgcc gcgcgtgccg aacggcaacc
     1741 cttcgatcca gtcgttctgc gacaggtcga gccgcggccg gtattcggtg ccgcgcggcg
     1801 agagcttcgt gtcgccgacg gcgagcgcgg cgaggcgcag cccgaccgtg agatagcgct
     1861 cgagcaagag cggcgacgtc ttcagagtat cggagacgtt gtcgaagccg tcgctcgtga
     1921 tgtcggcggg cagcagcgtt tcgacgtcga cgggaaaacc gagcaagtcc cgcaccgcgt
     1981 tgccgtacgc cgcgcgcgtc aggcggcgca cgggcgcgcc ggccggctcc ggctcgctcg
     2041 cgtcgagcgt cgtctcgaga taggacacca tgccggcgac ggcttcgtca tcgggccgcg
     2101 gctcgccggg cggcggcatg aggcccgccc tgagcttgtg aatgaccttc tcccattgcg
     2161 cgcgctgcgc ctcggggttc aggagatccg ggttctcgag cacgaggtcg gcctcgcgtt
     2221 cggcggcgct gtggcaatcc gtgcagtact tctcgatcgt cgcgccgtgc tcggccgcga
     2281 gccgctcgga cgactgcgtg cacgagacga gcggcgcggc gcagagcatc gggagcacga
     2341 cgacgacgac gcaacgccgc aacgaaactg cgctcatcgc gccgccgtcc tctgctcttc
     2401 cttgccgcgc gcgccgagct ccatgagcgc ggcggccgcg ggattgtcgg ccggcggtcg
     2461 cgtgcgcagc cgcggcggca ggcggccgac cgcatagtcg agcggcgtga acccgtcgcg
     2521 atcgccgatg ttgagatccg cgcccttctc ggccaggtgc ttgatgaccc cgggatacgc
     2581 gcgcagcgcg gcgacgtgca gcgccgtcga gccgatgtcg ttgcccatcg tcagatccgc
     2641 gccgcggctc aagagaaagt cgagcgccgc gatcgcgtcg tcctcgagcg ggcggcggtc
     2701 ggcttcgatc agcgacggca gctcgggccc cgtcatcacg gcaaccagcg gcgtgattcc
     2761 gccgcgcttg tcgtcgcgca tcgaggggtc cgcgccgccg tcgatgagca cgcgcatcgc
     2821 tccgacgtcg gctgagcgcg ccgcgcggat cagcgcgacg tcggtgatct tcggcggcgg
     2881 cggcacgaag cgcgcgtcgc gatcgagcag cggctgcggc atgcggccca tgagatcggc
     2941 gcccttcgac agcatcaagg cgagcagatc caacgagagc tccgggcccg tcggaacctg
     3001 atactcgccg acgtagttga cgcggttgcg cgcctcgacg acgcccaaga gcccgccgtc
     3061 gtcggcgctg gcaccgccct cgacgagcac cgtggccgtg tcgaagtgca gattgtcgag
     3121 cgcgatcaca attggcgtga agccctccgg cgttttcgcg tcgaggtcgg ccttacccgc
     3181 gatcagcgag cgcaatgcgg agttggcgcc gctgcgcgcc gcgaacatga ccggcgtgaa
     3241 gcccgcgacc ggaatctcga cgtagtagcg gacccgcgat tcgcgctccg gcaaatcgtt
     3301 gatcggagtc ctggcgttca cgtcggcgcc ggccgcgatc aacgcggccg cgacctcgcc
     3361 gcgatcgtgc gcggccgcgt acatgagcgg cgtctggcct tgccactgct cgacgacgtc
     3421 gacgttcgcg ccgcgctcga tcagcagccg aaccgcggcg atgccgctcg tgcgcgacgc
     3481 gagcatgagc ggagtctcgc cctgcggcag cgcaagatcg acgctcgcgc cggcctcgag
     3541 aagcgtgtgc agcaggtcgg cgtccgcgcg cagcgcggcc tcgtgcatcg gcgcgacgcc
     3601 atagcggttc gcgagattgg gatcggcttt ggcgccgagc agcgcacgca cgctttgcgt
     3661 gtcgccgaaa tgcgcggcgt agacgagcgg cgtcgagccg tccgcggccc gcgcgttcgc
     3721 gtcggcgccg gccgcgagca gcgcgcggac ttccgtcgca ttcgccttct gcgccgcgtc
     3781 gagcagcttc gcgtcgagct cggagtcggc ccacgccccc aatgaaatga gcaacgccga
     3841 cgcgaaagca ccgatcttgg acaacgtcgt cacggcttcc ccgctgtgat caagccggag
     3901 cgtattgata atttgacaaa ccgtcaataa c
//
LOCUS       DASSNN010000115         3807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_152054, whole genome shotgun sequence.
ACCESSION   DASSNN010000115 DASSNN010000000
VERSION     DASSNN010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152054"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VFL84_02045"
     CDS             <1..1055
                     /locus_tag="VFL84_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607430.1"
                     /translation="KVASVAVQADLGQEIRVSADIPCEEGVLVAVEVLNNKSRYNTIE
                     LTSGRMAEVKKGDVVVGALGHRKALFGYSGHLPTQLAPGDTVQLLNLGGVIGICDSIN
                     PSHGEPFNCKVLGCVLDFPYLGQRVGVPARVSTEPLKLDVPLDTHGVPVVAIAGTCMN
                     SGKTAAACAIVSRFRHNGLTVDAFKSTGVALRRDILAMEDSGARHIAMFSDFGICATT
                     SKNAPALTRSLLNELSDEKPDAIVFELGDGLLGAYGVEAILRDKEIVKQITAMVLCAN
                     DPVGAWGGAKLLREEFGIEPTVISGPATDNQVGIDIIRERSNVAALNAMTNAAELGDL
                     LQASIGLGSRTTKATA"
     gene            1052..2113
                     /gene="argC"
                     /locus_tag="VFL84_02050"
     CDS             1052..2113
                     /gene="argC"
                     /locus_tag="VFL84_02050"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET7607431.1"
                     /translation="MSTTQIPAIVLGGSGYVAGEFLRLIAGHPELALGGVVSTSQAGE
                     PVGKTFPHLAPTYPDTRFVSLDAAVERLNTAPRWLLLSGAPHGASAQLIDQVLNAGAS
                     AGTEVVVVDASADFRFADPAAYAAVYGHAHATPARLKEFTCAIPEQLARIETPHAAHP
                     GCFATTMLLPLVPLAALGLADEFYVSAVTGSTGAGRTPRDTTHHPLRQSNLFAYQALK
                     HRHAPEVRALIKASTGRDVRLHFVPHSGPFARGIHATIFLHAKERLTAAQVNDALREY
                     YRGATFVRVEDEPPKMKDIAATNYAALHAVADGDTIVVCSVTDNLLKGAAGGSLQWAN
                     RLLGFPESTGLTAPAAGWL"
     gene            2113..3288
                     /locus_tag="VFL84_02055"
     CDS             2113..3288
                     /locus_tag="VFL84_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7607432.1"
                     /translation="MSVAKHEIAAVSAHLTEVYPMLPFAPVRGHGVWLENAAGRRILD
                     FYGGHAVAALGYGHRELSETIARASRDLVFQTNALPLQVRDEAADKLAAFGPPGLGRV
                     FFVNSGAEANENALRLAFKVTGRSKVVAMEHSFHGRTAAAAAVTYGAMKTWYGFPRTP
                     FDVVFVPRNDLAALDAAVDSSTAAVIIEPVQGLAGAFDFAPQFVHAARAACDRAQALL
                     ILDEVQTGMGRLGAPFGAQLHGVRPDLLTVAKGLGGGFPCGAVLMPHSIAKELKAGAL
                     GTTFGGGPVACAAIKTVIDVINRDGLCANVRDVSAAIKATCRVGPVESIQGRGFLLGL
                     KTKPKAAAVRHALLARDILVGTSADPNMLRLLPPLILSTEHVQRLAKALEELTDAPL"
     gene            3275..>3807
                     /locus_tag="VFL84_02060"
     CDS             3275..>3807
                     /locus_tag="VFL84_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607433.1"
                     /translation="MRRFNDLADFSREEIKELIALAARLDAKPEPRALEGKVLSLLFL
                     SPSLRTLSSFQAAMLRLGGGSFVISPDMSIHGLESRYGIVMDGAAAEHVREAIPVIAS
                     YGDAMGVRAFAERRDLAHDLADNDFKALTGLIDKPWINMESAIDHPCQSLADWKTLDD
                     LKVPQDGKFVLSWAYHPR"
BASE COUNT          607 a         1320 c         1281 g          599 t
ORIGIN      
        1 acaaagtcgc ctccgtcgcc gttcaagcgg atctcggtca agagatccga gtgagcgccg
       61 acattccctg tgaggaaggc gttctcgtcg ccgtcgaggt gctcaacaac aagtcgcgct
      121 acaacacgat cgagctcacg agcggccgca tggccgaggt caagaagggc gacgtcgtgg
      181 tcggcgcgct cgggcatcgc aaggcgttgt tcggctactc gggccatttg ccgacgcagc
      241 tcgcgccggg cgacacggtc cagctcctga acttgggcgg cgtcatcggc atttgcgact
      301 cgatcaatcc gtcgcacggc gagccgttca actgcaaagt gctgggctgc gtgctcgact
      361 tcccgtactt ggggcagcgc gtcggcgtgc ccgcgcgcgt ctcgaccgag ccgctcaagc
      421 tcgacgtgcc gctcgacacg cacggcgtgc ccgtcgtcgc gattgccggc acctgcatga
      481 actccggcaa gacggccgcc gcgtgcgcga tcgtcagccg cttccggcac aacggcttga
      541 ccgtcgacgc tttcaagtcg acgggcgtcg cgctgcgccg cgacatcctc gccatggagg
      601 attcgggcgc ccgccacatc gcgatgttct cggacttcgg catctgcgcg acgacgtcga
      661 agaacgcgcc tgcgctgacg cgctcgctgc tgaacgagct gtccgacgag aagcccgacg
      721 cgatcgtgtt cgagctcggc gatggactgc tcggcgcgta cggcgtcgag gcgatcctgc
      781 gcgacaagga gatcgtcaag caaataaccg cgatggtgct ctgtgcgaac gacccggtcg
      841 gcgcatgggg cggcgcgaag ctgttgcgcg aggagttcgg catcgagccg accgtgatct
      901 cgggcccggc caccgacaac caagtcggca tcgacatcat ccgcgaacgc tccaacgtcg
      961 ccgcgttgaa cgcgatgacg aatgcggccg agctcggcga cctgctgcaa gcgagcatcg
     1021 gcttgggatc ccggacgacg aaggccacgg catgagcacg acgcaaattc cggcaatcgt
     1081 gctcggcggc tccggctatg tggccggcga gttcttgcgg ctcatcgcgg gccatcccga
     1141 gctcgctctc ggcggcgtcg tctcgacgag ccaagcgggc gagccggtcg gcaagacgtt
     1201 cccccacttg gccccgacgt atcccgatac gcggttcgtc tcgctcgacg ccgcggtcga
     1261 gcggctcaac accgcgccgc gctggctgct gttgtccggc gcgccgcacg gcgcgtcggc
     1321 gcagctcatc gaccaggtct tgaacgcggg cgcgagcgcc ggcaccgagg tcgtcgtcgt
     1381 cgacgcatcg gccgacttcc gtttcgcgga ccccgccgct tacgcggccg tttacggcca
     1441 cgcgcacgcg acgccggcac ggctcaagga gttcacgtgc gcgataccgg agcagctcgc
     1501 gcgaatcgag actccgcatg cggcgcaccc gggctgcttc gcgacgacga tgctgctgcc
     1561 gctcgtgccg ctcgctgcgc ttgggctggc cgacgagttc tacgtcagcg ccgtcacggg
     1621 cagcacagga gccgggcgca cgccgcgcga cacgacgcac catccgctac gccagagcaa
     1681 cctgttcgcc tatcaggcgt tgaaacaccg ccacgctccc gaggtgcgcg cgctgatcaa
     1741 ggcctcgacg gggcgcgacg tgcgtctgca cttcgtgccg cactcggggc cgttcgcgcg
     1801 cggtattcac gcgacgatat tcttgcacgc caaggagcgg ctgaccgcgg cgcaggtcaa
     1861 cgacgcgctg cgcgagtact atcgcggcgc gacgttcgtc cgcgtcgaag acgagccgcc
     1921 gaagatgaag gacatcgcgg cgacgaacta cgcggcgctg cacgccgtgg ccgacggcga
     1981 tacgatcgtc gtctgctcgg tgacggacaa cctgctcaaa ggagccgccg gcggcagctt
     2041 gcaatgggcg aatcggctct tgggatttcc tgaaagcacc ggcttgaccg cgccggcagc
     2101 ggggtggctc tgatgagcgt ggcgaaacac gagattgccg cagtgagcgc gcatctcacc
     2161 gaagtctatc cgatgctgcc gttcgcgccc gtgcgcgggc acggcgtttg gctcgagaac
     2221 gcagctggcc ggcgaatcct cgacttctac ggcggccacg ccgtcgcggc gctcggctac
     2281 ggtcatcgcg agctctccga gacgatcgcg cgcgcgagcc gcgatctcgt cttccagacg
     2341 aacgcgctgc cgctgcaagt tcgcgatgag gcggccgaca agctcgccgc gttcggcccg
     2401 ccgggtttgg gccgcgtgtt cttcgtcaac agcggcgccg aggcgaacga gaatgcgctg
     2461 cggctcgcgt tcaaggtcac cgggcgcagc aaggtcgtcg cgatggagca cagcttccac
     2521 ggccgaacgg ctgcagccgc agccgtgacc tacggcgcca tgaaaacgtg gtatggcttc
     2581 ccgcgcacgc cgttcgacgt cgtgttcgtg ccgcgcaacg acttggccgc gctcgacgcg
     2641 gccgtcgact cgagcacggc cgccgtcatc atcgagcccg tccagggcct cgccggcgcg
     2701 ttcgatttcg cgccgcaatt cgtgcatgcc gcgcgcgcgg cgtgcgaccg cgcccaagcg
     2761 ttgctgattc tcgacgaggt gcagacgggc atgggccggc tcggcgctcc gttcggcgcg
     2821 cagctgcacg gcgtgcgccc cgacctgctg acggtcgcga aggggctcgg cggcggcttc
     2881 ccgtgcggcg cggtcttgat gccgcactcg atagcgaagg agctgaaggc cggggcgctc
     2941 ggcacgacgt tcggcggcgg acccgtcgct tgcgccgcga tcaagaccgt catcgacgtg
     3001 atcaaccgcg acggtctctg cgccaacgtg cgcgacgtca gcgccgcgat caaggccacg
     3061 tgccgcgtcg gacccgtcga gagcattcaa gggcgcgggt tcttgctcgg tctgaagact
     3121 aagcccaagg ccgccgcggt gcgccacgcg ctgctcgcgc gcgacatcct cgtcggcacg
     3181 agcgccgatc cgaacatgct gcggctgttg ccgccgctga tactctcgac cgaacacgtg
     3241 caacgcctgg cgaaggccct ggaggaactg accgatgcgc cgctttaacg acctcgcgga
     3301 cttctcgcga gaagagatca aggagctcat cgcgctcgcg gcgcggctcg atgcgaaacc
     3361 ggagccccgc gcgctcgaag gcaaggtgct gtcgctgctg ttcctgagcc cgtccctgcg
     3421 cacgctgtcg tcgttccaag ccgcgatgct gcgcctcggc ggcggctcgt tcgtgatctc
     3481 gccggacatg tcgatccacg ggctcgagtc gcgctacggc atcgtcatgg acggggccgc
     3541 ggccgagcac gtccgcgagg cgatcccggt catcgcgtcc tatggcgacg ccatgggcgt
     3601 gcgcgcgttc gccgagcggc gcgatctcgc gcacgacctc gccgacaacg acttcaaagc
     3661 gctgacgggg ctcatcgaca agccctggat caacatggag tcggcgatcg accatccgtg
     3721 ccagagcctc gccgactgga agacgctcga cgacctcaaa gtcccgcagg acggcaagtt
     3781 cgtgctgtcg tgggcctatc acccgcg
//
LOCUS       DASSNN010000116         6960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_24646, whole genome shotgun sequence.
ACCESSION   DASSNN010000116 DASSNN010000000
VERSION     DASSNN010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6960
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24646"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFL84_02065"
     CDS             complement(<1..548)
                     /locus_tag="VFL84_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7607434.1"
                     /translation="MPIALEGDAERGKIIGQTCTGCHGIPGYYNAQPAYHVPKLGGQN
                     ADYIEVALQGYRRGTRGHDTMRAQASSLSDQDIADVAAYFASFEGEPERARSHASKAM
                     IEAGREKSTTCQSCHGAEGVAAAPQWPNLAGQHETYLQEALERYKTGQRVDMVMNPLI
                     APLDEQSIEELAAFFAAQPHLY"
     gene            complement(747..2561)
                     /locus_tag="VFL84_02070"
     CDS             complement(747..2561)
                     /locus_tag="VFL84_02070"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010984475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HET7607435.1"
                     /translation="MALLEVDNLNVRFATPDGEVHAVKDLSFALEQGETLGIVGESGS
                     GKSQTVMSMIGLLAANGRATGVARFAGRDLLAMAPKELRTIRGRKIAMIFQDPMTSLN
                     PYLMIEKQMVAAVRAHERVSFKAARARCLSMLNAVAIPEPDLRLKRYPHELSGGMRQR
                     VMIATSLLLEPEVLVADEPTTALDVTVQAQILELLKDLKRRFNTAIVLITHDLGVVAG
                     LADRVLVMHGGEIKEQGQVRDVFYRPQHDYTRMLLNAVPRLDSTPPAAPLPAQSGAPL
                     IEVRAVKVHFPVPGRGLLGSRETLRAVDGVDLTLAPGETLGVVGESGSGKSTLARAIL
                     KLVPATAGSVAVLGRDLAELSRHELRAIKRNLQVVFQDPLAALNPRMTVGDIVSEPLW
                     THRPELDAAAVRAEVVDVLRRVGLTGRELNRYSHEFSGGQCQRIGIARALVLKPKVVV
                     CDEPVSALDVSIQREIVRLLLELRRELGLALVFIAHDLAVVRQLSHRVMVMYLGCTME
                     VASRDDLYAHPRHPYTQALMSAVPLPDPDAERARSRAVLHGELPSPIRPPSGCRFRTR
                     CPYAAERCAAEVPPLRPFGNALVACHFAEEIAGLRAKL"
     gene            complement(2564..3448)
                     /locus_tag="VFL84_02075"
     CDS             complement(2564..3448)
                     /locus_tag="VFL84_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019934062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7607436.1"
                     /translation="MSAAAATVPPQVVGRSLWADAWAKLKRNRAAIVSAWIIAAMAVL
                     VVVGPYFSRYAFDYTDFASASTGPSFRTGHFFGTDTLGRDLFVRTLYGGRISLMVGIV
                     ATAVSLLIGVTYGAVAGYFGGRVDSVMMRIVDILYALPFMFFVILLMVFFGRNIVLIF
                     VAIGAINWLDMARIVRGQTLSLKHREFVEAAQAQGVGAAAIIRRHIVPNLLGVVAVYV
                     TLTIPQVILVESFLSFLGLGVQEPMTSWGALVNDGSREIESAQWMLIFPAAFLATTLF
                     CFNFLGDGMRDALDPKDR"
     gene            complement(3445..4359)
                     /gene="oppB"
                     /locus_tag="VFL84_02080"
     CDS             complement(3445..4359)
                     /gene="oppB"
                     /locus_tag="VFL84_02080"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HET7607437.1"
                     /translation="MLSYTIRRLLGAIPTLLILIALAFLLIRAAPGGPFDRERSLPPE
                     VEANLRAAYHLDEPLYEQFGRYLWGLLHFDFGPSFQYRDYTVTELIAAGFPVSLTLGL
                     CALVVALFIGVTAGSVAALRQNSGVDHGVMALSMTGISIPNFVMAPLLVLVFAVYLRW
                     LPAGGLGERSLVLPVIALALPQIAYLSRLTRGSMIEVLRSNFVRTARAQGLPTWQVIV
                     RHALKPALLPVVSYLGPAAAQIITGSVVIEQIFSRPGLGRYFVTGALNRDYTLVMGVV
                     IFYGVLIILFNFLVDLAYAWLDPKVRYR"
     gene            complement(4385..6034)
                     /locus_tag="VFL84_02085"
     CDS             complement(4385..6034)
                     /locus_tag="VFL84_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7607438.1"
                     /translation="MRKRYGVLAMLAAGLVASCGGGSDGGSAGGPAAQIGGASGKELA
                     EAQVLHVGNGAEPQTLDPHRAEETSSFGVLVDIYEGLVSESPNGDLIPGVASQWTIGD
                     DGKTYVFNLRPEARWSNGDPLTAHDFVFSFRRGVDPKTLSVYSYILSPLKNADDITAG
                     KRPPEELGVRAIDDHTLEIELANPTPYFLELLAHSMCYPVHRGMLEAHGDQWTRPGNL
                     VGNGAFKLDEWVVQSHIKLVRNPYYWDDAGTKLDEVWLHATEDLTAELQRFRARELDM
                     TYRIPAAQIGFIRENLADELDIAPYLGSYYYGFNLTRPPFKDQPKLRRALSLAVNRDV
                     ITKQVLNLGQISSYGVVPPIKGYAQQQMLEATWTQEAREAEAKRLYAEAGYGPDNPLR
                     AQLMYNTGEDHRRIAVALASMWKQVLGVETELLNQEWKVFLDTRNQKKDTQVYRSGWI
                     GDYTDPFTFLEIFRSTAGQNDTGYLSSEYDRLVTASQSALEPHARMELLQKAERELLA
                     DMPILPLYIYVTPRLVQRWVGGYEPNLLDHQRHQYMYVLKH"
     gene            complement(6099..>6960)
                     /locus_tag="VFL84_02090"
     CDS             complement(6099..>6960)
                     /locus_tag="VFL84_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019019283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7607439.1"
                     /translation="FFDPRDLKSSLETKAVANLFFAGQINGTTGYEEAAAQGIVAGIN
                     AGLRARGREPWSPRRDEAYIGVLIDDLITRGTSEPYRMFTSRAEFRLSLREDNADLRL
                     TPVGRELGLVPDERWRAFEARREWLAKEAARLDDVVVKPADVPQGGAFAEPLTREASA
                     YELLRRPGVGYADVAALRRVSPSPELAALDAERVEQWTASLVIDAHYAGYVARQSVEI
                     ERQKREAGTVLPQDFDYGGVAGLSNELREKLARIRPNDIGQAARISGMTPAALSLLLI
                     HVKKRRRLTA"
BASE COUNT         1145 a         2310 c         2400 g         1105 t
ORIGIN      
        1 ttgtagaggt gtggctgcgc ggcgaagaac gccgcgagct cttcgatgct ttgctcgtcg
       61 agcggcgcga tcagcgggtt catgaccatg tcgacacgct ggccggtttt gtaacgctcg
      121 agcgcctcct gcaggtaggt ctcgtgctgg cccgcgagat tcggccactg cggcgccgcg
      181 gcgacgcctt cggcgccgtg gcacgactgg cacgtcgtcg atttctcgcg cccggcctcg
      241 atcatggcct tcgacgcatg cgagcgcgcg cgctccggct caccctcgaa gctcgcgaag
      301 taggccgcga cgtcggcgat gtcctggtcg gagagcgacg acgcctgcgc gcgcatcgtg
      361 tcgtggccgc gcgtgccgcg ccggtagcct tgcagcgcga cctcgatgta gtcggcgttc
      421 tggccgccga gcttcggcac gtgatacgcg ggctgcgcgt tgtagtagcc cgggatcccg
      481 tggcaacccg tgcaggtctg gccgatgatc ttgccgcgct cggcgtcgcc ctcgagcgcg
      541 atcggcatca gcggcgccgg cggagcgctg ccctcttgcg cgaaagcggg cgccccatat
      601 gcgcaggcca gaatggtcaa agcggcggcg gccaaagagc gaaacacggc ggggggctcc
      661 aggagattcc cttctcgaag ccgcgcatct tagccaaaag cccgccgcga tggcaggaga
      721 gcttcggttt tccgaagggg cgagggctaa agcttcgctc gcagccccgc gatttcttcg
      781 gcgaagtgac acgcgaccaa tgcgtttccg aacggccgca acggcggcac ctcggcggcg
      841 cagcgctcgg cggcatacgg gcagcgcgtg cggaaccggc agccgctcgg cggccggatc
      901 ggcgacggta gctcgccatg cagcaccgcg cgcgagcgcg cgcgctcggc gtcggggtcc
      961 ggcagaggca cggcgctcat gagcgcctgc gtgtatggat ggcgcgggtg cgcgtagaga
     1021 tcgtcgcggc tcgcgacctc catcgtgcag ccgagataca tgaccatgac gcggtgcgac
     1081 agctggcgca cgacggcgag atcgtgcgcg atgaatacga gcgcgagccc gagctcgcgc
     1141 cgcagctcga gcagcagccg cacgatctca cgctggatcg aaacgtcgag cgcgctgacg
     1201 ggctcgtcgc aaacgacgac cttcggcttc agcacgagcg cgcgcgcaat gccgatccgt
     1261 tgacattgcc cgcccgagaa ctcgtggctg tagcggttta gctcgcggcc ggtcagaccg
     1321 acgcgccgca gcacgtcgac gacctcggcc ctgactgccg cggcgtcgag ctccggccgg
     1381 tgcgtccata agggctcgct gacgatgtcg ccgacggtca tgcgcgggtt cagcgccgcg
     1441 agcggatcct ggaacacgac ctggaggttg cgcttgatcg ctcgcagctc gtggcgcgaa
     1501 agctcggcga ggtcgcggcc gagcacggcg acggagccgg ccgtcgcggg cacgagcttc
     1561 aggatcgcgc gcgcgagcgt cgacttgccg ctgcccgact cgccgacgac gccgagcgtc
     1621 tcgcccggcg cgagcgtgag gtcgacgccg tcgacggccc gcaacgtctc gcgggaccca
     1681 agcaagccgc gcccgggcac cgggaagtgc accttgaccg cgcgcacctc gatcagcggg
     1741 gcgccggatt gcgcgggcag cggcgctgcc ggcggcgtgc tgtcgagccg cggcacggcg
     1801 ttcagcagca tgcgcgtgta gtcgtgctgc gggcgataga acacgtcgcg cacttggccc
     1861 tgctccttga tctcgccgcc gtgcatgacg agcacgcggt cggcgaggcc cgcgacgacg
     1921 ccgagatcgt gcgtgatcag cacgatcgcg gtattgaagc ggcgcttcag gtctttgagg
     1981 agctcgagga tttgcgcctg caccgtgacg tcgagcgccg tcgtcggctc gtcggcgacg
     2041 agcacctcgg gctcgagcaa taggctcgtc gcgatcatga cgcgctggcg catgccgccg
     2101 gacagctcgt gcggatagcg cttcaagcgc aagtccggct ccgggatcgc gacggcgttc
     2161 agcatcgaga ggcaccgcgc gcgcgccgcc ttgaacgaga cgcgctcgtg cgcgcggacc
     2221 gccgcgacca tctgcttctc gatcatcaga tacgggttca gcgacgtcat cggatcctgg
     2281 aagatcatcg caatcttgcg gccgcggatc gtgcgcagct ctttcggcgc catcgcgagc
     2341 aagtcgcggc ccgcgaagcg cgcgacgccg gtcgcgcggc cgttcgcggc gagcaggccg
     2401 atcatgctca tgaccgtctg gctcttgccg gagcccgatt cgccgacgat cccgagcgtc
     2461 tcgccctgct cgagcgcgaa gctcaagtcc ttgacggcgt gtacctcgcc gtcgggcgtc
     2521 gcgaagcgaa cgttcaagtt gtcgacttca agcagagcca tcgctatcga tctttggggt
     2581 cgagcgcgtc gcgcatgccg tcgcccagga agttgaagca gaacagcgtc gtcgcgagga
     2641 acgcggccgg aaagatcagc atccactgcg cgctctcgat ctcgcggctg ccgtcgttga
     2701 cgagcgcgcc ccacgacgtc atcggctctt gcacgcccaa cccgaggaag ctcaagaacg
     2761 actcgacgag gatgacttgc ggaatcgtca gcgtgacgta gacggccacg acgccgagca
     2821 agttcggcac gatgtggcgg cggatgatgg ccgccgctcc gacgccttgc gcctgcgccg
     2881 cctcgacgaa ctcgcggtgc ttcagcgaga gcgtctggcc gcgcacgatg cgcgccatgt
     2941 cgagccagtt gatcgcgccg atcgcgacga agatcagcac gatgttgcgg ccgaagaaga
     3001 ccatcagcag gatcacgaag aacatgaacg gcagcgcgta gaggatgtcg acgatgcgca
     3061 tcatcacgct gtcgacgcgg ccgccgaagt agccggcgac ggcgccgtag gtcacgccga
     3121 tcagaaggct cacggccgtc gcgacgatgc cgaccatgag cgagatccgg ccgccgtaga
     3181 gcgtgcgcac gaacaagtcg cggccgagcg tgtcggtgcc gaagaagtgt ccggtgcgga
     3241 agctcgggcc cgtcgacgcg ctcgcgaaat cggtgtagtc gaatgcgtaa cggctgaagt
     3301 aggggccgac gacgacgagc acggccatcg cggcgatgat ccaggcgctg acgatcgccg
     3361 cgcggttgcg cttgagcttc gcccaagcgt cggcccagag gctgcggccg acgacttgcg
     3421 gcgggaccgt tgccgcagcc gcgctcatcg gtacctcact ttcgggtcga gccacgcgta
     3481 cgcgaggtcg accaagaagt tgaacaggat gatcagcacg ccatagaaga tgacgacgcc
     3541 catgacgagc gtgtagtcgc ggttcagtgc gccggtcacg aaatagcggc cgagcccggg
     3601 gcggctgaag atctgctcga tgacgaccga gcccgtgatg atctgcgccg cggccggccc
     3661 gagatacgag acgacgggca gcagcgccgg tttcagcgcg tgcctgacga tgacctgcca
     3721 cgtcggcagg ccctgggccc gcgccgtgcg cacgaagttg ctgcgcagca cctcgatcat
     3781 gctgccgcgc gtcaaccgcg acaggtacgc gatctgcggc aacgcgagcg cgatgacggg
     3841 cagcacgaga ctccgttcgc ccaagccgcc ggccggcagc cagcgcaaat agacggcaaa
     3901 gacgagcacg agcagcggcg ccatgacgaa attcgggatc gagatgcccg tcatcgagag
     3961 cgccatcacg ccgtggtcta cgcctgaatt ctgccgaagc gcggcgaccg agccggccgt
     4021 gacgccgatg aatagcgcca cgacgagcgc gcagagccca agcgtcagcg acacggggaa
     4081 gcccgcggcg atgagctccg tgacggtgta atcgcggtac tggaacgacg ggccgaaatc
     4141 gaagtgcagc aggccccaca gatagcggcc gaactgctcg tagagcggct cgtccaggtg
     4201 gtacgccgcg cgcaggttcg cttcgacctc gggcggcagg ctgcgctctc tgtcgaacgg
     4261 gccgccgggc gcggcgcgaa tcaacaagaa cgcgagagcg atcagaatca gcagcgtcgg
     4321 aatcgcgccg agcagccgcc gtatcgtgta agagagcaag tcgtgcgtgt cgcgctagtg
     4381 ctggttagtg cttcaggacg tacatgtact ggtgccgctg gtggtcgagc aggttcggct
     4441 cgtaaccgcc gacccatcgc tggacgagcc ggggcgtcac atagatgtag agcggcagga
     4501 tcggcatatc cgcgagcagc tcgcgctcgg ccttttgcag cagctccatt cgcgcgtgcg
     4561 gctcgagcgc gctttgcgac gcggtgacga gccggtcgta ttcggagctc aagtagcccg
     4621 tgtcgttctg cccggccgtc gagcgaaaga tctcgaggaa cgtgaatgga tcggtgtagt
     4681 cgccgatcca cccggatcgg tagacctgcg tgtccttctt ttggttgcgc gtatcgagga
     4741 agaccttcca ttcctggttc agcagctcgg tctcgacgcc gagcacctgc ttccacatcg
     4801 acgcgagcgc gacggcgatg cggcgatggt cctcacccgt gttgtacatg agttgcgcgc
     4861 gcaacgggtt gtcgggaccg tagccggctt cggcgtacag ccgcttggct tcggcctcgc
     4921 gggcttcctg cgtccaggtc gcctcgagca tctgctgctg cgcgtatccc ttgatcggcg
     4981 gcacgacgcc gtaggacgaa atctgcccca agttcaggac ctgcttcgtg atgacgtcgc
     5041 ggttgacggc gagcgagagc gcgcggcgca gcttcggctg gtctttgaac ggcgggcgcg
     5101 tcaggttgaa gccgtagtaa tagctgccga gatacggcgc gatgtcgagc tcgtccgcga
     5161 ggttctcgcg gatgaagccg atctgcgccg ccggaatccg gtacgtcatg tcgagctcgc
     5221 gcgcgcggaa gcgctggagc tcggccgtca ggtcctcggt cgcatgtagc cagacttcgt
     5281 cgagcttcgt gccggcgtcg tcccaatagt aggggttgcg cacgagcttg atgtgcgact
     5341 gcacgaccca ctcgtcgagc ttgaacgcgc cgttgccgac gagattgccg ggcctcgtcc
     5401 attgatcgcc gtgcgcctcg agcatgccgc ggtgcaccgg atagcacatc gagtgcgcga
     5461 gcagctcgag gaaatacggc gtcggattcg cgagctcgat ctcgagcgtg tggtcgtcga
     5521 tcgcgcgaac tccgagctcc tcgggcggcc gtttgccggc cgtgatgtcg tcggcgtttt
     5581 tgagcggcga caagatgtag ctgtagacgc tgagcgtctt cggatcgacg ccgcgccgga
     5641 acgaaaacac gaaatcgtgc gccgtgaggg ggtcgccgtt cgaccaacgc gcctcgggcc
     5701 gcagattgaa gacatacgtc ttgccgtcgt cgccgatcgt ccactgcgat gcgacgcccg
     5761 ggatcaaatc gccgttcggc gactcgctga ccagcccctc gtagatgtcg acgagcacac
     5821 cgaagctcga ggtctcttcg gcccgatgcg ggtcgagcgt ttgcggctcg gcgccgttgc
     5881 cgacgtgcag gacttgagcc tcggcgagct ccttgccgct cgcgccgccg atctgtgccg
     5941 ccggaccgcc cgccgagccg ccgtcggagc cgccgccgca gctcgcgacg agacccgccg
     6001 ccagcatcgc caaaacgccg tagcgcttcc tcatctacgt ctcctcggca atgacttctg
     6061 cattgcctgc cacgcgtggg ccgagccgtg gccctggctc aagccgtgag ccggcggcgc
     6121 ttcttcacgt gaatcaatag aagcgagagc gcggccggcg tcatgcccga gatgcgcgcc
     6181 gcttgcccga tgtcgttcgg ccgaatgcgc gcgagcttct cgcgcagctc gttcgagagc
     6241 cccgcgacgc caccgtagtc gaaatcctgc ggcagcaccg tgcctgcttc gcgcttttgc
     6301 cgctcgatct cgacgctctg cctcgcgaca taaccggcgt agtgcgcgtc gatgaccaag
     6361 ctggccgtcc actgctcgac gcgctcggcg tcgagcgcgg caagctccgg cgatggactc
     6421 acgcggcgca gcgcggcgac gtcggcgtag ccgacgccgg gccggcgcag cagctcgtag
     6481 gccgaggcct cgcgcgtcag cggctcggcg aaggcgccgc cctgcggcac gtccgccggc
     6541 ttcacgacga cgtcgtcgag gcgcgcggcc tccttcgcga gccattcgcg gcgcgcctcg
     6601 aatgcgcgcc agcgctcgtc gggcacgagc ccgagctcgc ggccgaccgg cgtcaggcgc
     6661 aagtcggcgt tgtcctcgcg caagctcagg cgaaactcgg cgcggctcgt gaacatgcga
     6721 tacggctcgc tcgtgccgcg cgtgatcagg tcgtcgatca gcacgccgat gtaggcctcg
     6781 tcgcggcgcg gcgaccaggg ctcgcgccct cgcgcgcgaa ggcccgcgtt gatgccggcc
     6841 acgatgcctt gcgcggccgc ctcctcgtag cccgtcgtgc cattgatctg gccggcgaaa
     6901 aacaggttcg cgacggcctt cgtctcgagc gacgacttga gatcgcgcgg atcgaaaaag
//
LOCUS       DASSNN010000117         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_152374, whole genome shotgun sequence.
ACCESSION   DASSNN010000117 DASSNN010000000
VERSION     DASSNN010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152374"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /gene="ilvC"
                     /locus_tag="VFL84_02095"
     CDS             <1..775
                     /gene="ilvC"
                     /locus_tag="VFL84_02095"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET7607440.1"
                     /translation="GAGLVAFLAPDLAQGQLYKDIEPNLKKGATLLFAHGFNIHYKLV
                     NPRADLDVILIAPKGPGDLVRRQYEQGRGVPCLMAVAQDASGKAQARALAYAHGIGGT
                     RGGVLATTFREETETDLFGEQAVLCGGATELIVRGFQTLVEAGYQPEVAYFECMHEMK
                     LIVDLLHEGGLKKMHQYVSDTAKYGDLTRGPRIIDAHVRENMKQVLKEVQDGTFAKQW
                     IEENRTGQKNYKRLMQEDLDQGIEKVGKELRGRMSWLQE"
     gene            845..1810
                     /locus_tag="VFL84_02100"
     CDS             845..1810
                     /locus_tag="VFL84_02100"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET7607441.1"
                     /translation="MPLKTSKYIWHEGKLVPWEQATIHVLSHALHYGSSVFEGIRVYK
                     TPSGAAGFRLTDHMRRLYHSAKIYRIKIPFSLDTLIGACRDVVRENGLLGGAYIRPIA
                     YRGYGEMGVAGNIEQPASVSVAAWEWGSYLGEGGLDQGVDVCVSSWQRVAPNTVPALA
                     KAGGNYLSSALVTLEARRLGFTEGIALNTAGFVSEGAGENLFLVQDGKLYTPPVASSI
                     LSGITRNTVIRLAESAGIEVLEQNIPRELLYIADEVFLTGTAAEITPVRSVDKIPVGE
                     GKRGPLTERLQKLFFGLFDGSTADRWNWLEPIGGEKAGRSRPVAV"
     gene            1819..>3128
                     /locus_tag="VFL84_02105"
     CDS             1819..>3128
                     /locus_tag="VFL84_02105"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET7607442.1"
                     /translation="MPSLFEKIWGKHVVVPESDDTPAVLYIDLHLLHEVTTPQAFDLL
                     RAQGLPVRRPDRCLATLDHSTPTVPVKTLKDLEIVSEPSAAKQVRQMEKNCKDFGIEL
                     RGFASRDRGIVHVMGPELGATQPGATIVCGDSHTSTHGAFGALAFGIGTTEVGHVLAT
                     QCLLQRKPKTLAINLSGKLPSDVTAKDLILKIIGTIGVGGGTGHVLEYRGEAIRALSM
                     EERMTVCNMSIEAGARAGMIAPDDTTYQYMAGRPLAPQGAAWDEALKRWRALPSDAGA
                     KFDKEVTIDVAKMRPMVTFGTNPGMVIPIDEAVPNDSDPAFRKALAYMQVEAGKPMLG
                     KPVDVVFIGSCTNGRLSDIREAARILRGRRVAERVRLLVVPGSEDVKRQAEAEGLDEV
                     VRRQSQFVIIDITGVELVDTATADRFIKLVTAVEYLGARCMLTGT"
BASE COUNT          569 a         1033 c         1055 g          471 t
ORIGIN      
        1 aggcgccggg ctcgtcgctt tcctcgcgcc cgatctggcg caagggcagc tctataaaga
       61 catcgagccg aacttgaaga aaggcgcgac gctcctgttc gcgcatggct tcaacattca
      121 ctacaagctc gtgaacccgc gtgccgatct cgacgtcatc ctgatcgcgc cgaagggccc
      181 cggcgatctc gtgcgccgtc aatacgagca aggccgcggc gtcccctgcc tgatggccgt
      241 cgcgcaagac gcgagcggca aggcgcaagc gcgcgcgctc gcctacgcgc acggcatcgg
      301 cggcacgcgc ggcggcgtgc tcgcgacgac gttccgcgag gagaccgaga ccgatttgtt
      361 cggcgagcaa gccgtgctct gcggcggcgc cacggagctg atcgtgcgcg gcttccaaac
      421 cctcgtcgag gccggctatc agcccgaggt cgcgtacttc gagtgcatgc acgagatgaa
      481 gctcatcgtc gacctgctgc acgaaggcgg tctcaagaag atgcatcagt acgtcagcga
      541 caccgccaag tacggcgacc tgacgcgcgg gccgcgcatc atcgacgcgc acgtgcgcga
      601 gaacatgaag caggtattga aagaggtcca agacggcacc ttcgcgaagc aatggatcga
      661 agagaaccga acgggccaga agaactacaa gcgcctgatg caggaagacc tcgaccaggg
      721 catcgagaag gtaggcaagg agctgcgcgg ccgcatgagc tggctgcagg aatgagagtg
      781 gggacggatt tcaaatccgt cccccctgtt ctgtggaacg aagtgaagag ctaggagcga
      841 cccaatgccc ctcaaaacga gcaagtacat ctggcacgaa ggcaagctgg ttccgtggga
      901 gcaggccacg atccacgtgt tgagccatgc cctgcactac ggctcgtcgg tattcgaggg
      961 cattcgcgtc tacaagacgc cgagcggagc cgcgggtttt cggctgacgg atcacatgcg
     1021 ccggctgtac cactccgcga agatctatcg catcaagatt ccgttctcgc tcgacacgct
     1081 catcggcgcg tgccgcgacg tcgtgcgcga gaacggcttg ctcggcggcg cgtacatccg
     1141 gccgatcgca tatcgcggct acggcgagat gggcgtcgcc ggcaacatcg agcagccggc
     1201 cagcgtctcg gtcgcggctt gggaatgggg ctcgtacctg ggcgaaggcg gcctcgatca
     1261 gggcgtcgac gtctgcgtct cgtcgtggca gcgcgtcgcg ccgaacacgg tgcccgcgct
     1321 cgcgaaggcc ggcggcaatt atctgtcgag cgcgctcgtg acgctcgagg cgcgccgcct
     1381 cgggttcacc gagggcatcg cgctgaacac ggcgggcttc gtcagcgaag gcgccggcga
     1441 gaacctgttc ctggtccagg acggcaagct ctacacgccg cccgtcgcgt cgtcgatcct
     1501 gtccggcatc acgcgcaaca cggtcattcg cctcgccgag tccgccggca tcgaggtgct
     1561 cgagcagaac atcccgcgcg agctcctgta catcgccgac gaggtgttcc tgaccggcac
     1621 cgccgccgag atcacgcccg tgcgctcggt cgacaagatt cccgtcggcg aaggcaagcg
     1681 cggcccgctg acggagcggc tgcagaagct gttcttcggc ctcttcgacg gcagcacggc
     1741 cgatcgctgg aattggctcg agccgatcgg cggcgagaag gccggccgct cgcggcccgt
     1801 cgcggtctag gagccgacgt gccgtcgctg ttcgaaaaga tttggggcaa gcacgtcgtc
     1861 gtgcccgaga gcgacgacac gcccgcggtc ctctacatcg acttgcacct gctgcacgag
     1921 gtcacgacgc cgcaggcgtt cgacttgctg cgcgcgcagg gcttgcccgt acggcggccc
     1981 gatcgctgcc tcgcgacgct cgatcactcg acgccgaccg tgccggtcaa gacgctgaag
     2041 gatctcgaga tcgtcagcga gccgtccgcc gcgaagcagg tgcggcagat ggagaagaac
     2101 tgcaaggatt tcggtatcga gctgcgcggc ttcgcgagcc gcgaccgcgg catcgtgcac
     2161 gtcatgggcc cggagctcgg cgcgacgcag cccggcgcga cgatcgtctg cggcgactcg
     2221 cacacgagca cgcacggcgc gttcggcgcg ctcgccttcg gcatcggcac gaccgaggtc
     2281 ggccacgtgc tcgcgacgca gtgcctgctg cagcgcaagc cgaagacgct cgcaatcaac
     2341 ttgagcggca agctgccgag cgacgtcacg gcgaaagact tgatcttgaa gatcatcggc
     2401 acgatcggcg tcggcggcgg cacgggtcac gtgctcgagt accgcggaga ggcgatccgc
     2461 gcgttgtcga tggaagagcg catgacggtc tgcaacatgt cgatcgaggc cggcgcgcgc
     2521 gccggcatga tcgcgcccga cgacacgacg tatcagtaca tggccggccg gccgctcgcg
     2581 ccgcagggcg cggcgtggga cgaggccctg aagcgttggc gcgcattgcc gagcgacgcc
     2641 ggcgcgaagt tcgacaagga agtcacgatc gacgtcgcca agatgcggcc gatggtcacg
     2701 ttcggcacga atcccggcat ggtcattccg atcgacgagg ccgtgcccaa cgacagcgac
     2761 cccgcgtttc gcaaagcgct cgcctacatg caggtcgagg ccggcaagcc gatgctgggc
     2821 aagcccgtcg acgtcgtgtt catcggctcg tgcacgaatg gacgcctgag cgacattcgc
     2881 gaggcggcgc gcatcctgcg cggccgccgc gtcgccgagc gcgtgcgctt gctcgtcgtg
     2941 ccgggctccg aggacgtcaa gcggcaggcc gaagccgaag gtctcgacga ggtcgtgcgc
     3001 cgacagtcgc agttcgtgat catcgacatc accggcgtcg agctggtcga cacggccacg
     3061 gcggaccgct tcatcaagct ggtcaccgcg gtcgagtacc tcggcgcccg ctgcatgctc
     3121 accggcac
//
LOCUS       DASSNN010000118         6331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_403360, whole genome shotgun sequence.
ACCESSION   DASSNN010000118 DASSNN010000000
VERSION     DASSNN010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403360"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VFL84_02110"
     CDS             complement(<1..1162)
                     /locus_tag="VFL84_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7607443.1"
                     /translation="MSPRPKLVRVAVPVPLADAFDYLAAADAALPPIGCRVRVPFGRG
                     ERIGLVVDHPAATDVAPARLKTIGAVLDAAPALGSELLSSLRWAAEYYHHPIGAVLSH
                     ALPGPLREGRAIDEPPEPTWQLTALGRAVDVASLPRGARQQARALHALRERALTASEL
                     ETRDVAAAALARLAAKGWIEPAPAPAPEPIPADRHGEPRGREPELTADQRAALDEIAA
                     TQGTANKFRAYLLHGVTGSGKTEVYLRLIAAQLGARRQTLLLVPEIGLTPQLVRRLRE
                     RFGECLAVLHSAVTERQRFDAWRRAYRGDAKLVVGTRSAVFAPLPSAGLIIVDEEHDP
                     SYKQQTGFRYSARDLAVVRAQRLEVPIVLGSATPSLETYNNALQGRYRKLTLP"
     gene            1322..1927
                     /locus_tag="VFL84_02115"
     CDS             1322..1927
                     /locus_tag="VFL84_02115"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7607444.1"
                     /translation="MGLALSIAQREASLGREGALIGALIEAFLRDVEARAYRIALAQI
                     RDRHEALDVVQDAMIRLVRRYASRPSVEWPPLFYRILQNRIRDVQRRRAVRARVLSFF
                     GGTASEDDHDPIAEAPAPRAEDPLEQVTADDAMQALEWALAELPVRQREAFVLRNFEG
                     LDVAQTAAAMGCTEGSVKTHYSRAVHRLREALAEHGEESTT"
     gene            1924..2307
                     /locus_tag="VFL84_02120"
     CDS             1924..2307
                     /locus_tag="VFL84_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607445.1"
                     /translation="MRNDTDGNSDFERRAKRAFDDSVAALDAATRSKLTQARYRALEE
                     QASARDRGWRSSLVPAGTVAATALVAFLVMWQQPQPDVEVAQAPLGDLEILLGEEDLE
                     MLDEELEFYGWLEAQPEFANADSVG"
     gene            2307..2594
                     /locus_tag="VFL84_02125"
     CDS             2307..2594
                     /locus_tag="VFL84_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607446.1"
                     /translation="MRGINAALLLLGCSWLTGVRAQEGGPERGRDAAPDLDFLEYLGA
                     WAEGDEEWLAIEELRRDDDADVEDTKDAKDSKDPKDAEEPDTERDDDHESK"
     gene            2581..3051
                     /locus_tag="VFL84_02130"
     CDS             2581..3051
                     /locus_tag="VFL84_02130"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HET7607447.1"
                     /translation="MKANRLVAISIALLALAGMASAQGVAWQDLSEAQRQLLAPHEQR
                     WADLDPQRQEQIARGAQRFLEMNRGARAQAQNRFEIWRGMTPEQRAAARQRYQEFRRL
                     PPAERQRLVDTYRRYRQLPPERRQELRQTFRQLTPQQRQTLREQRTRQPRPTPR"
     gene            complement(3048..4433)
                     /locus_tag="VFL84_02135"
     CDS             complement(3048..4433)
                     /locus_tag="VFL84_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET7607448.1"
                     /translation="MGTLGAATAIQASYFVAAALFIFGLKRMSSPVTARSGIVWAGVG
                     MVVATLITFLTPGMGNYALMTVAIAVGGIAAWVTGRRVAMTDMPQMIALYNGMGGGAA
                     GAIAAVELFSGEARGTAVLALGVLGGIIGAVSFSGSLIAFGKLQGLIKRSLRFRGQNI
                     VNLVILAGTVVAGSVIVLHGGEGVEGHVTLFLVLALVLGVTMTLPIGGADMPVVISLY
                     NALTGLAVAFEGFVLNNAAMIIAGTVVGSAGTLLTQLMAKAMNRSLGNVLFSGFGETA
                     ATGSAGPAGTLKAIETSDAAVMLGFSEKVIIVPGYGMAVAQAQHKVWELCQALMARGV
                     TVKFAIHPVAGRMPGHMNVLLAEAGVPYDLIYDEAEINAEFETADVALIIGANDVVNP
                     VARTDKSSPIYGMPILNADKAKNVIVIKRGQGAGFSGIENALFYLDHTRMLYGDGQAK
                     VAELVQAVKAL"
     gene            complement(4433..4741)
                     /locus_tag="VFL84_02140"
     CDS             complement(4433..4741)
                     /locus_tag="VFL84_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET7607449.1"
                     /translation="MIDGFIALYIFMLAAFTGYEVISRVPVILHTPLMSGSNFVHGIV
                     LVGAMVAMGRAETALEITIAFIGVVLAAGNAVGGYAVTERMLEMFKSSKGVSKNKGKG
                     "
     gene            complement(4755..5885)
                     /locus_tag="VFL84_02145"
     CDS             complement(4755..5885)
                     /locus_tag="VFL84_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET7607450.1"
                     /translation="MPLSIGVLRETSAHEARVALTPDVAAKLLELGVTIHMQSGAGEA
                     SHFPDALFKGVQLHAEPVAVARSSGALLTVQPPPLSAVEALPAAAVVVGFLQPHRELP
                     LVRALRDRKLTSFAVELIPRISRAQSMDALSSQAAAAGYKAALLAANALDKFLPMLTT
                     AAGTIRPAQVLVIGAGVAGLQAIATARRLGAIVEGYDVRSATKEQIQSLGAKFVDTGV
                     AAEGTGGYARELTAEEKAKQQEILDKHIAKADVVITTAAVPGRTAPRIISKGAVAAMG
                     AGAVIVDLAAESGGNCELTVAGETVVTPNGVKILGPRNLPSELARHASQMYARNLLNF
                     LSPAIKNGELAIDWQDEVFAQSVLTHDGQVKHAPTAKLLEAR"
     gene            6111..>6331
                     /locus_tag="VFL84_02150"
     CDS             6111..>6331
                     /locus_tag="VFL84_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET7607451.1"
                     /translation="MPLEWIDYRIAATLYHSGQVAYACGSPIYRIRGGYCAKSGDRSV
                     IEVSSIIATHGDTRTLERNRERYTPPLNNR"
BASE COUNT         1042 a         2111 c         2218 g          960 t
ORIGIN      
        1 gcggaagagt caactttcga tagcgcccct gtagcgcgtt gttgtacgtc tcgagcgacg
       61 gcgtcgcgga gccgagcacg atcggcacct cgagccgctg cgctcgcacg acggcgagat
      121 cgcgcgccga gtagcgaaat cccgtctgct gcttatacga agggtcgtgc tcctcgtcga
      181 cgatgatgag cccggccgac ggcagcggtg cgaacacggc ggagcgcgtg ccgacgacga
      241 gcttggcgtc gccgcgatac gcgcgccgcc aggcgtcgaa tcgctgccgt tccgtgacgg
      301 ccgaatgcag caccgccagg cattcgccga agcgctcgcg caagcgccgc acgagctgcg
      361 gcgtcaggcc tatctcgggc acgagcagta gcgtctggcg gcgcgcgccg agctgcgccg
      421 cgatcaggcg caagtagacc tcggtcttgc cgctgccggt gacgccgtgc agcaggtagg
      481 cgcgaaattt gttcgcggtg ccctgcgtcg ccgcgatctc gtcgagcgcc gcgcgctgat
      541 cggccgtcag ctcgggctcg cgcccccgcg gctcgccgtg ccggtcggcg ggtatgggct
      601 ccggcgccgg cgccggcgcc ggctcgatcc agcccttcgc cgcgaggcgc gcgagcgcgg
      661 cagccgcgac gtcgcgcgtc tcgagctcgc tcgccgtgag cgccctttct cgcagcgcgt
      721 gcagcgcgcg cgcttgctgg cgcgccccgc gcggcaagct cgcgacgtcg acggcgcggc
      781 cgagcgccgt cagctgccag gtcggctcgg gcggctcgtc gatggcgcgg ccctcgcgca
      841 gcggccccgg caacgcgtgg ctcaacaccg cgccgatggg atggtggtaa tactcggccg
      901 cccagcgcag cgaggacaag agctccgagc ctagggcggg cgccgcatcg agcacggcgc
      961 cgatcgtctt caggcgcgcc ggcgcgacgt ccgtcgcggc ggggtggtcg acgacgagac
     1021 cgatccgctc gccgcggccg aacggcacgc gcacgcggca gccgatcggc ggcaatgcgg
     1081 catcggcggc cgccaggtaa tcgaaagcgt cggccagcgg caccggcacc gcgaccctga
     1141 cgagcttcgg gcgtgggctc atggggccgc gattatgtga atagaaacct tcagaaagtc
     1201 aatgacttaa cggcagcggt ttcctgcgtc aaccgcttcc cgaggccggc cgttatggcc
     1261 tttcatcgac cggtggaaag gggccggact ggttgcggac gtacgccacg gagcggcgat
     1321 tgtggggtta gcattgagca tcgctcagcg cgaagcgagc ctcggcaggg agggggctct
     1381 catcggagct ctaatcgagg ctttcttgcg cgacgtcgaa gcgcgagcgt atcggattgc
     1441 gctcgcgcaa attcgcgacc gccatgaggc gctcgacgtc gttcaagacg cgatgatccg
     1501 gctcgtgcgc cgctatgcga gccggccgag cgtggagtgg ccgccgctgt tctatcggat
     1561 cctgcagaac cggatccgcg acgtgcagcg gcggcgcgcg gtgcgcgcgc gggtattgag
     1621 cttcttcggc ggcacggcgt cggaggacga tcacgacccg atcgccgagg cgcccgcgcc
     1681 gcgagcggag gacccgctcg agcaggtcac ggccgacgac gcgatgcagg cgctcgagtg
     1741 ggcgctcgcg gagttgccgg tgcggcagcg cgaagcgttc gtgctgcgca acttcgaggg
     1801 gctcgacgtc gcgcagacgg cggcggcgat gggttgcacg gaaggcagcg tgaagacgca
     1861 ctattctcga gccgtgcatc gcttgcgcga ggcgctcgcg gagcacggcg aggagagcac
     1921 gacatgagga acgataccga cggtaacagc gatttcgagc ggcgcgcgaa gcgcgcgttc
     1981 gacgacagcg tggccgcgct cgacgcggcg acgcgctcga agctgacgca agcgcgctat
     2041 cgcgcgctcg aagagcaggc gtcggcgcgc gaccgcggct ggcgctcgtc gctcgtgccg
     2101 gcgggcacgg tggccgcgac ggcgctcgtc gcttttctcg tcatgtggca gcagccgcag
     2161 ccggatgtcg aggtggcgca ggcgccgtta ggcgacctcg agatactgct cggcgaagaa
     2221 gatctcgaga tgctcgacga ggagctcgag ttctacggct ggctcgaggc gcagcccgag
     2281 ttcgcgaatg cagacagcgt cggttgatgc gcgggatcaa tgcagcgctg ctgctgctcg
     2341 gctgctcgtg gctcacgggc gttcgcgcgc aggaaggcgg cccggagcgg ggccgcgacg
     2401 cggcgccgga cctcgacttt ctcgaatatc tcggcgcttg ggccgaaggc gacgaagagt
     2461 ggctggcgat cgaagaatta cgcagggacg acgacgcgga cgtcgaggac accaaggacg
     2521 caaaggactc aaaggacccg aaggacgccg aagagcccga cactgagaga gatgacgacc
     2581 atgaaagcaa ataggctcgt tgcgatctcg atcgcattgc tcgcgctggc cgggatggca
     2641 agcgcgcaag gtgtcgcgtg gcaagacttg agcgaagcgc aacggcagct gctcgcgccg
     2701 cacgagcagc gctgggccga cttggacccg cagcggcagg agcagatcgc gcgcggcgcc
     2761 cagcgtttcc tcgagatgaa ccgcggcgct cgcgcgcaag cgcaaaatcg cttcgagatc
     2821 tggcgcggca tgacgcccga gcaacgagcc gcggcgcggc agcgctatca agagttccgc
     2881 cgcctgccgc cggcggagcg acagcggctc gtcgacacgt atcgccgtta tcgccagttg
     2941 ccgccggagc ggcgccagga gctgcgccag acgttccggc agctgacgcc gcagcagcgc
     3001 cagacgctac gcgagcagcg cacgcgccag ccgcggccga cgccgcgcta gagcgccttc
     3061 accgcctgca cgagctcggc gaccttcgcc tggccgtcgc cgtacagcat gcgtgtgtga
     3121 tcgagataga acagcgcgtt ctcgatcccg gagaagcccg cgccctgacc gcgcttgatg
     3181 acgatgacgt tcttcgcttt gtcggcgttc aagatcggca tgccgtagat cgggctcgac
     3241 ttgtccgtgc gcgcgacggg gttcacgacg tcgttggcgc caatgatcag cgcgacgtcg
     3301 gccgtctcga attccgcgtt gatctccgcc tcgtcgtaga tcaagtcgta cggcacgccg
     3361 gcttcggcga gcagcacgtt catgtggccc ggcattcgac ccgcgaccgg atggatcgcg
     3421 aacttgacgg tgacgccgcg cgccattagt gcctggcaga gctcccagac cttgtgctgc
     3481 gcctgggcga cggccatgcc gtagcccggc acgatgatga ccttctccga gaaaccgagc
     3541 atgacggccg catcggaggt ttcgatggcc ttcagggtgc cggccggccc ggccgaaccc
     3601 gtagcggcgg tctcgccgaa gccgctgaac agcacgttgc cgagcgagcg gttcatggcc
     3661 ttggccatga gctgggtcag aagcgtgccg gccgagccga cgaccgtgcc cgcgatgatc
     3721 atcgcggcgt tgttgagcac gaagccttcg aacgcgacgg cgagccccgt cagcgcgttg
     3781 tagagcgaga tcacgacggg catgtccgcg ccgccgatag gcagcgtcat cgtgacgccg
     3841 agaacgagcg cgagcacgag gaacagcgtg acgtgccctt cgaccccctc gccgccgtgc
     3901 aggacgatga cggagcccgc gacgacggtg ccggcgagga ttaccaagtt cacgatgttc
     3961 tgaccgcgga accgcaagct gcgcttgatc agcccttgca gcttgccgaa cgcgatcaag
     4021 ctgcccgaga acgagacggc gccgatgatg ccgccgagca cgccgagcgc gagcaccgcg
     4081 gtgccgcgcg cctcgcccga gaacagctcg acggccgcga tcgcgcccgc ggcgccgccg
     4141 cccatgccgt tatagagcgc gatcatctgc ggcatgtccg tcatcgcgac tcgccggccg
     4201 gtgacccacg ccgcgatgcc gccgaccgcg atcgcgacgg tcatcagcgc gtagttgccc
     4261 atgccgggcg tcaggaacgt gatcagcgtc gcgacgacca tgccgacgcc ggcccagacg
     4321 atgccgctgc gcgccgtgac cggcgagctc atgcgcttca agccgaagat gaacagcgcc
     4381 gccgcgacga agtagctcgc ctggatcgcc gtcgccgcgc cgagcgtgcc catcagccct
     4441 tgcccttgtt tttggatacg cccttgctgc tcttgaacat ctcgagcatg cgctcggtga
     4501 cggcgtagcc gccgacggcg ttgcccgcgg cgagcacgac gccgatgaac gcgatcgtga
     4561 tctcgagcgc cgtctcggct cgtcccatcg cgaccatcgc gccgacgagc acgatgccgt
     4621 gcacgaagtt cgagcccgac atgagcggcg tgtgcaagat cacgggcacg cgcgagatga
     4681 cctcgtagcc cgtgaaggcc gcgagcatga agatgtacaa cgcgatgaat ccgtcgatca
     4741 tgagagaacc tcggtcatcg tgcctccagc agcttcgcgg tgggcgcatg cttgacctgg
     4801 ccgtcgtgtg tcagcacgga ctgcgcgaac acttcgtcct gccagtcgat cgcgagctcg
     4861 ccgttcttga tggccggcga caggaagttc agcaaattgc gcgcatacat ctggctcgcg
     4921 tgccgcgcga gctccgaagg caggttgcgc gggcccaaga tcttgactcc gttcggcgtc
     4981 acgacggtct cgccggcgac cgtgagctcg cagttgccgc cgctctcggc cgcgaggtcg
     5041 acgatgaccg cgcccgcgcc catcgcggcc accgcgccct tcgagatgat gcgcggtgcc
     5101 gtgcgcccgg gcacggcggc cgtcgtgatc acgacgtcgg ccttcgcgat gtgcttgtcg
     5161 aggatttcct gctgcttggc cttttcctcg gccgtgagct cgcgcgcgta cccgcccgtg
     5221 ccttcggccg cgacgccggt gtcgacgaac ttcgcgccga gcgattggat ctgctccttc
     5281 gttgcggagc gcacgtcgta tccctcgacg atcgcgccga gccggcgcgc cgtcgcaatg
     5341 gcctgcaggc ccgcgacgcc ggcgccgatc acgagcactt gtgcgggccg gatcgtgccg
     5401 gcggccgtcg tcagcatcgg caggaacttg tcgagcgcgt ttgcggcgag cagcgcggcc
     5461 ttgtagccgg cggccgccgc ctgcgacgac aacgcgtcca tcgattgggc gcgcgagatg
     5521 cgcgggatca gctcgaccgc gaagctcgtg agcttgcggt cgcgcagcgc tcgcacgagc
     5581 ggcagctcgc ggtgcggctg aaggaaccct acgacgacgg cggcggccgg gagcgcttcg
     5641 acggccgaga gcggcggcgg ttggaccgtc agcaacgcgc ccgaggagcg cgccacggca
     5701 accggctcgg catgaagctg cacgcctttg aacagcgcgt cggggaagtg gctcgcctcg
     5761 ccggcgccgc tctgcatatg gatcgtgacg ccgagctcga ggagcttggc cgcgacatcg
     5821 ggtgtcaagg cgacccgtgc ctcgtgggca gaggtttccc gtagcacacc gatactgagt
     5881 ggcatccgac gctccgtcta ttggagttgg tagtcgcgtt gcggccaggc aacgcgtaaa
     5941 gcggaatcgg ccggcctcgt cggaaatcga tgatgacaca aaggttttac gtaggccatt
     6001 gaatcttggc gatctctcgg agaaacttgg gccgggctca gtcggctcgt tggatacaag
     6061 gacacgtgca cgctctgact caagaagttc tgcgcaccga tgtcgcggga atgccactcg
     6121 agtggatcga ctaccgcatc gcggcgacgc tatatcacag cggccaggtg gcctatgcct
     6181 gcggctcgcc gatttatcga atccgaggcg gctattgcgc gaaatccggc gatcgcagcg
     6241 tcatcgaggt cagctcgatc attgcgacgc acggcgacac gcggacgctc gagcgcaacc
     6301 gcgagcgtta cacgccgccg ctcaacaacc g
//
LOCUS       DASSNN010000119         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1033437, whole genome shotgun sequence.
ACCESSION   DASSNN010000119 DASSNN010000000
VERSION     DASSNN010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033437"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..2476)
                     /locus_tag="VFL84_02155"
     CDS             complement(140..2476)
                     /locus_tag="VFL84_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7607452.1"
                     /translation="MTKAGWRLYWRDLVTNGRVPASELCEVRVEHVTPAIRADRVIGS
                     TIRLTAQGLSLLAAAAALGQRVGGMPADAAAEPDRANLETIVITAQKRLEDVQDVAMA
                     VAAFDGGSLRNAGVTDAMQLQTVVPSLTYVATGYMAQPYLRGIGTRQSTVGLEPSVAT
                     YIDDRYVARPFAAMFDMQDVERVEVLKGPQAVLYGRNAAGGAIRAITKDPAERSFEIA
                     GKAGDYSARRFGIIAGGPLTDRWRGQISGTIERRDGFAANLVPSGRPAADDLDREAVR
                     GKLLFDLTDTVHAKLGLSWWKYTDWTGRDLVAAGLPQANRGVALYGGVTSRERGEFAT
                     ALAGDNDLREAAADLRFDVRLGNLDFASVTTYADSSFEQTFDVDASSVKLIDLHATEP
                     SNTWTQDFQLVSPQSARTPWLVGLQLYRQDASNLYVFAEPVSAQPAFPPGTDISNGLQ
                     HVETDAHALFAQVALPFRKRWTATLGGRWSSEDKSATLDAAPDAVTNVPTPYADAENW
                     DAFTPRVALEYRGDFGLAYLSYSRGFKTGGYNYPASANPVLNPETLDSYELGIKTGLA
                     GDRLKLRSALFHYDFEDLQVSRGGAGAFITTENAASATVQGLEVELSAAVTAALSLEA
                     GVALIDSEYTDYVAGVLVPLTAPPYGSAPLPGGLDVRGQSLLRSPDEAAYVGVLYELS
                     LPSGARLPIRVDYSYKGDYYFDFSPVPETEWLKQDAYGVLNARVAYASASGGWEVGIW
                     CTNLTDSTYYEDAVVQSASSRVSYADPRAYGIDFRLRR"
     gene            2544..>3042
                     /locus_tag="VFL84_02160"
     CDS             2544..>3042
                     /locus_tag="VFL84_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7607453.1"
                     /translation="MPKPKTSTKAPFIQAVVHATDFSAAAERAFAHALGVALFTRAEL
                     AILHVASGKSSDWRGFPAVRETLERWRLLESGSSRAAVFEELGVSVTKIGLQSRFPAL
                     AVTEYLSQRPADLLVVATEGRDGVARWLRGSIAEAMARWSRTMTLFVPADSDHDVVAL
                     ADGNIT"
BASE COUNT          485 a         1011 c         1037 g          509 t
ORIGIN      
        1 cagatgatcg tggaccggca caacggtcag ctgtccgtct cctccgacgt gggcaagggc
       61 acgcgcttca agatcactct gcctttcgac ccgacggcct cggccgtcgg cgccgatgcg
      121 cgcgtcgcgg ccggctcctt caacgccgaa gcctgaagtc gattccgtag gcgcgcgggt
      181 ccgcgtacga cacgcgcgac gaggcactct ggaccacggc gtcctcatag taggtcgagt
      241 ccgtcaggtt cgtgcaccaa atcccaacct cccaaccgcc gctcgcgctt gcatacgcga
      301 cgcgcgcgtt gagcacgccg taggcgtcct gcttcagcca ttcggtttcg gggaccggcg
      361 agaaatcgaa gtagtagtcg cctttgtacg agtaatcgac tctgatcggc agccgcgcgc
      421 cgctcgggag cgagagctcg tacaggactc cgacgtaggc cgcctcgtcg ggcgagcgca
      481 gcaacgattg cccgcgaacg tcgaggccgc ccggcaacgg cgcgctgccg tagggcgggg
      541 cggtcaacgg cacgagcacg ccggcgacgt aatcggtgta ctcgctgtcg atgagcgcga
      601 cgccggcctc gagggacaag gcggcggtga ccgccgcgct gagctcgacc tcgaggccct
      661 gcacggtggc gctcgccgca ttctcggtcg tgatgaacgc gccggcgccg ccgcgcgata
      721 cctgaaggtc ctcgaagtcg taatggaata gcgccgaccg cagcttcaat cggtcgccgg
      781 cgagacccgt cttgatcccg agctcgtagc tgtcgagcgt ctcggggttc agcaccggat
      841 tcgcggatgc cggatagttg tagccgccgg tcttgaagcc gcgtgagtag ctcaaatatg
      901 cgagcccgaa gtcgccgcga tactcgagcg cgacgcgcgg cgtgaaagcg tcccagttct
      961 ctgcatcggc gtacggagtt ggcacgttgg tcacggcgtc aggggcggcg tcgagcgtgg
     1021 cggacttgtc ctcgctgctc cagcggccgc cgagcgtcgc cgtccaccgc ttccggaacg
     1081 gcaacgccac ctgcgcgaat aacgcgtgcg cgtccgtctc gacgtgttgc aagccgtttg
     1141 aaatgtcggt gccgggcgga aatgccggct gcgccgaaac gggttcggcg aacacgtaca
     1201 ggttgctcgc gtcttggcga tagagttgca aaccgacgag ccacggcgtg cgcgcggact
     1261 gcggcgacac gagctgaaaa tcttgcgtcc aggtgttgct gggctcggtc gcgtgcaggt
     1321 cgatcagctt gacgctcgat gcgtcgacgt cgaaggtctg ctcgaagctg ctgtccgcgt
     1381 aggtcgtcac cgacgcgaaa tccaagttgc cgagccgcac gtcgaagcgc aggtcggcgg
     1441 ccgcctcgcg caagtcgttg tcgcctgcga gcgccgtcgc gaactcgccg cgctcgcggc
     1501 tcgtgacgcc gccgtacagc gcgacgccgc gattcgcctg cggcaagccg gcggcgacga
     1561 gatcgcgccc ggtccaatcg gtgtatttcc accacgaaag gccgagcttc gcgtgcacgg
     1621 tgtccgtgag gtcgaagagc agcttgccgc gaacggcttc ccggtcgaga tcgtcggccg
     1681 cgggacgtcc gctcggcacg agattcgccg cgaacccgtc gcgccgctcg atcgtgccgg
     1741 agatctgccc gcgccaccgg tccgtgagcg gacctccggc gatgatgccg aagcggcgag
     1801 ccgaatagtc gccggccttc ccggcgatct cgaaggatcg ctcggccggg tccttcgtga
     1861 tcgcgcggat tgcgccgccc gccgcgttgc gcccgtagag cacggcttgc ggacctttca
     1921 gcacctcgac gcgctcgacg tcctgcatgt cgaacatcgc ggcaaacggc cgcgcgacgt
     1981 aacggtcgtc gatgtaagtc gcgacggacg gctcgaggcc gacggtcgat tgccgcgtgc
     2041 cgatgccgcg caaatacggc tgcgccatgt agcccgtcgc gacgtaggtc aggctgggga
     2101 cgaccgtttg cagctgcatc gcgtcggtga cgccggcgtt gcgcagcgat ccgccgtcga
     2161 acgcggccac tgccatcgcg acgtcttgca cgtcctcgag gcgcttctgg gccgtgatga
     2221 cgatcgtctc gagattcgca cggtcgggtt cggcggcggc atccgccggc atgccgccga
     2281 cacgttgccc aagggctgca gcggccgcga gcagcgagag cccttgggcc gtcagccgta
     2341 tcgtcgagcc aatgacgcga tcggcgcgaa tcgccggcgt aacgtgttcc actcgcactt
     2401 cgcagagctc cgaggccggc actctaccgt tcgtgacgag gtcgcgccaa tagagccgcc
     2461 agccggcctt cgtcaataga acgttcggct actacgtaag gtcattgagg ccgagcgata
     2521 gtcggctcga ccgggaggtc cgcgtgccga agccgaagac gtcaacgaaa gcgccgttca
     2581 ttcaagcggt cgtgcacgcg accgacttct ccgccgccgc tgagcgcgcg ttcgcgcacg
     2641 ctctgggtgt cgcgcttttc acgagggcgg agctcgcgat cctgcatgtt gcgtccggca
     2701 agtcgagcga ctggcgcggc ttcccggccg tgcgcgagac gctcgaacgc tggcggctac
     2761 tcgagtccgg cagcagccgc gccgccgtgt tcgaggagct cggcgtgagc gtgacgaaga
     2821 tcgggctgca gagccgcttc ccggcgctgg ccgtgacgga atatctctcg caacggccgg
     2881 ccgatcttct ggttgtcgca accgagggac gcgacggagt tgcgcgctgg ttgcgtggct
     2941 ctatcgccga agcgatggct aggtggtccc gaacgatgac gctgttcgtg cccgcggatt
     3001 ccgaccatga cgtcgtggct ctggcagacg gcaacatcac gc
//
LOCUS       DASSNN010000120         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1033788, whole genome shotgun sequence.
ACCESSION   DASSNN010000120 DASSNN010000000
VERSION     DASSNN010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033788"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..991)
                     /locus_tag="VFL84_02165"
     CDS             complement(89..991)
                     /locus_tag="VFL84_02165"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HET7607454.1"
                     /translation="MLLTRCPECDTTFRVTDETLKKASGQVRCGRCASVFNAYAELKG
                     AGAKPLEAEPRGQREKPPAAKAPEPPAPKVREPPAGGPDAVPAADEQEARDRHAISAT
                     EVDRVLTHDAAEPVPYVWLQPEPPPLSRWWTVATALAAVALGVQLIHHFRFEIAGHPT
                     FGPVLTAVYEQFGAGIVPSWDVHQYEIIDWAATAEPNSRGVGTLKITARIQNHGPLRQ
                     PYPSVQLRLKDRYETAVGSRMFTPAEYLPHDTAHGPLMLPGETARAEIEVVDPGPDAY
                     GFELDVCIEVEASLVTCGTDEVFL"
     gene            complement(1041..1916)
                     /gene="prmA"
                     /locus_tag="VFL84_02170"
     CDS             complement(1041..1916)
                     /gene="prmA"
                     /locus_tag="VFL84_02170"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET7607455.1"
                     /translation="MSRQRLKLALAAEDVPTAEALLTLAGAETISLRDAADDPVYEPE
                     PSSTPLWPTVAVEALFAQGTELAAVVEVLTATFHGASVSVDALDDSAWQPRLDEAVKP
                     RPIGTRLKLAPADDPQLPGDRRVVRINMGLAFGTGEHPTTALCLEWLERHVASRMTML
                     DYGCGSGILALAALALGARFAYAVDNDPQALTAARANAALNGAAERLFVGAPEALPAV
                     TVDVLAANILARPLIELAPAFAERVAPGGMLVLSGVLEPQAPRVAAAYAPYLTSVEQT
                     VRDGWVLLAGSRKAR"
     gene            complement(1921..>2091)
                     /locus_tag="VFL84_02175"
     CDS             complement(1921..>2091)
                     /locus_tag="VFL84_02175"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017223159.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET7607456.1"
                     /translation="GEHRRAAIARMTTALHEIVVDGISTNIPLHQEIFQHGAFKAGGT
                     DIHYLERRLGLK"
BASE COUNT          307 a          704 c          719 g          361 t
ORIGIN      
        1 ttcttgcagt agaagtaccg acccagggtg cgggcagcat agcacgacgc ccgcgaattt
       61 tgcttcgctg cgcgcgcgaa tttttttgtc aaagaaatac ttcgtcggta ccgcaggtta
      121 ctaaggaagc ttcgacttcg atacaaacat cgagctcgaa tccgtacgca tcgggacctg
      181 gatcgacgac ttcgatctcg gcgcgcgccg tttcacccgg caacatcagc ggtccatgcg
      241 cagtgtcatg cggaagatat tccgcgggcg tgaacatgcg gctgccgacc gcggtttcgt
      301 atcgatcctt gagacgcaat tgcaccgacg gatacggttg gcgcagcggc ccgtgatttt
      361 gaattctcgc cgtgatcttc aaggtgccga cgccgcgcga gttcggctcc gcggtcgcgg
      421 cccaatcgat gatctcgtat tgatgcacgt cccagctcgg cacgataccc gcgccgaatt
      481 gctcgtagac cgccgtgagc acggggccga acgtcggatg gccggcgatc tcgaagcgaa
      541 agtgatggat cagctgtacg ccgagcgcaa ccgcggcgag cgccgtcgcc acggtccacc
      601 agcggctcag cggcggcggc tccggttgca gccaaacgta ggggaccggc tcggccgcgt
      661 cgtgcgtcag cacgcggtcg acctcggtgg cgctgatggc gtgtcggtcg cgcgcctctt
      721 gttcgtccgc tgcggggacc gcgtcgggcc cgccggcggg cggttcccgc actttcgggg
      781 cgggcggctc cggcgctttc gcggcgggcg gcttctctcg ctggccgcgc ggctccgcct
      841 cgagcggttt ggcgccggct cccttgagct cggcgtaggc gttgaacacg ctcgcgcaac
      901 ggccgcaccg tacttgcccg ctcgccttct tcagcgtttc gtcggtgaca cggaacgtcg
      961 tgtcgcattc ggggcagcgc gtcagcaaca tgggcgtagc atgactcgtg acgcggcgat
     1021 tctagcgttt ctcggcgcgg ttaacgcgcc ttgcggctgc ctgcgagcag gacccagccg
     1081 tcgcggaccg tttgctccac gctcgtcaga tacggcgcat aggcggctgc gacgcgcggc
     1141 gcttgcggct cgagaacgcc cgatagcacg agcatgccgc cgggcgcgac gcgctccgca
     1201 aaagccggtg cgagctcgat gagcggcctc gcaaggatgt tcgcggcgag cacgtcgacg
     1261 gtgacggcgg gcagcgcctc gggcgcgccg acgaacaggc gctcggcggc gccgttcagc
     1321 gcggcgttcg ccctcgcggc cgtcaacgct tgcgggtcgt tgtcgaccgc gtacgcgaag
     1381 cgcgcgccga gcgcgagtgc cgcgagcgcg aggattccgg agccgcagcc gtagtcgagc
     1441 atcgtcatcc ggctcgccac gtggcgctcc agccactcga ggcagagcgc cgtcgtcgga
     1501 tgctcgccgg ttccgaacgc gagccccatg ttgatgcgca cgacgcgtcg gtcgcccggc
     1561 agttgcggat cgtcggccgg cgcgagcttt agccgcgtgc cgatcggccg cggcttgacc
     1621 gcctcgtcga gccttggctg ccaggccgag tcgtcgagcg cgtcaacgct cacagaggcg
     1681 ccgtgaaacg tcgcggtaag gacctcgacg accgccgcca actccgtgcc ctgcgcgaat
     1741 agcgcctcga ccgctacggt cggccacaac ggcgtcgagc tcggctccgg ctcgtagacg
     1801 ggatcgtccg ccgcgtcgcg cagcgagatg gtctcggcgc cggcgagcgt cagcagagcc
     1861 tctgcggtcg gcacgtcctc ggccgcgagc gcgagcttca gtcgttgccg actcaacgcg
     1921 tcacttcagc ccgagccgcc gctccagata gtgaatgtcg gtcccgccgg ccttgaaggc
     1981 gccgtgttgg aagatttcct ggtggagcgg aatgttcgtg ctgatgccgt cgacgacgat
     2041 ctcgtgcagc gccgtcgtca tgcgcgcgat cgcggcgcgg cggtgctcgc c
//
LOCUS       DASSNN010000121         4887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_153988, whole genome shotgun sequence.
ACCESSION   DASSNN010000121 DASSNN010000000
VERSION     DASSNN010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153988"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1142)
                     /gene="mraY"
                     /locus_tag="VFL84_02180"
     CDS             complement(54..1142)
                     /gene="mraY"
                     /locus_tag="VFL84_02180"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET7607457.1"
                     /translation="MLIYVADYLTELYSGFNVFSYLTTRAILGALTALVLSFAFGPYL
                     IQRLTMQRIGQQIRQVGPESHFPKAGTPTMGGALILLAIAVSTLLWADLENRFVLLVL
                     GGTLYFGVIGFVDDYKKVVLRNSKGLSVRAKLFWQTIGAFGLAILFYVTAEDPAVEHS
                     LLIPYIPGITIPLGAVGYVVFTALVIVCASNGVNLTDGLDGLAIMPSVLVGTALGIFA
                     YAAGNVIYSSYLGIPYVAGSGELLVFCAALAGAGLGFLWFNTYPAQVFMGDIGALALG
                     AALGLVAVAVRQELVLFVMGGVFVVESVSVIIQVASYKMTGRRIFRMAPLHHHFELKG
                     WAEPKVIVRFWIITVILVLIGLASLKIR"
     gene            complement(1144..2502)
                     /gene="murF"
                     /locus_tag="VFL84_02185"
     CDS             complement(1144..2502)
                     /gene="murF"
                     /locus_tag="VFL84_02185"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET7607458.1"
                     /translation="MMPRTLAAVAREVEGRLVGADHTFGAVTTDTRALVSGSLFVAIP
                     GDRFDGNDFVDEAFAKGAAGALVSRLAASALPQIEVRDSRRALGAMARAWRASFTIPV
                     VAVTGSSGKTTVKELIASILGVSRSVCVTQGNLNNDIGVPLTLMRLTAEHDAAVIELG
                     ANHAGEIDYLAGLAQPTVGVITNAAAAHLEGFGSLHGVCVAKGELLDHLPRAGTAVLN
                     ADDQFRSDWVARSRCEFTVTFGFAPSADCRVLGSPHFLANGSEFRMHLPDGEETDVWL
                     PLLGRQNVANALAAAAAAQAVGTSVEDIVAGLARAAPVRGRLRAVQGRNGATVVDDSY
                     NANPGSVRAAIDHLAALPGKRILVLGNMAELGPTGPALHREIGEYARGRCDALYAIGD
                     LAGEAAKAFGVDGRSVADIEAARAALDPLLAGDVTVLVKGSRVMGLDRLVKTLEAEPR
                     TT"
     gene            complement(2499..3965)
                     /locus_tag="VFL84_02190"
     CDS             complement(2499..3965)
                     /locus_tag="VFL84_02190"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET7607459.1"
                     /translation="MAAQALRERTLGDLLGKAAGSYSRLAITDLTLDSRAVRPGAAFV
                     ALRGGREHGIKYARAALDAGAAIVLYEPGEDSGEPPRPSLAVPGLKSRLGELARAFFM
                     PGAAPTIVGVTGTNGKTTVAYLLAQVLSQPQRPCAYVGTLGYGVPPALTAHSLTTPDC
                     LTLHRELAELGAPRAAMEVSSHALSQDRIAGLEFHTAVFTNLTRDHLDEHGDLKSYGE
                     AKRKLFTIPNLKVAVLNADDAFSATIAAGLAPSCTLLRTSVRSSAVELSARLERSTLA
                     GVELAVSGKFGTARLPSKLIGTFNAENLLSALGALLAQGMELPAACAALAAAKPAPGR
                     MEVLGGPPGHAWVVVDYAHTPDALLRVLTTLEDAATGELWCVFGCGGDRDRGKRPMMG
                     AVAADLADRIVLTDDNPRSEDPAGIVDDIRAGVGDHPRVSVIHDRRAAIASAVERARV
                     GDVVLVAGKGHEAEQIIGHERRPFSDRAVVAELLGGAR"
     gene            complement(3965..>4887)
                     /locus_tag="VFL84_02195"
     CDS             complement(3965..>4887)
                     /locus_tag="VFL84_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET7607460.1"
                     /translation="SQNPNAERSEYRPDNYRNRAVTDPFEPGSSFKPLVMAVALESGS
                     YGPRTPIDTSSPITVDSYIIKDPENLGRITLTKVLVKSSNLGAARVALTMKPAAIADV
                     LTRFGIGRLTDSAFPGESAGVLVDPKHWSTARQATLSYGYGLTVTTLQLARAYAAIAA
                     GGVVRPVSLLALDEAPPGQRVISADTAADLTSMLEAVVAKDGTGYRAAVRNYHVAGKT
                     GTTQKAGVGGYDVDRHGAIFAGFAPATDPRLVVVVMIDEPQGDAYHGGDIAAPVFANI
                     VSGALRVLAVPPDALPAPAMTVVAQAQVRP"
BASE COUNT          828 a         1678 c         1676 g          705 t
ORIGIN      
        1 cgagccaccc cgccgtcgac gtaagctccg aagcgtcagc gccgtcgccg ccatcaacgg
       61 atcttcaagc tcgccaaccc gatcaacacc aagatcacgg tgatgatcca aaagcgcacg
      121 atcactttgg gctcggccca acccttgagc tcgaagtgat ggtgtagcgg cgccatgcga
      181 aaaatgcgcc ggccggtcat cttgtaggag gcaacctgga tgatgactga gacggattcg
      241 acgacgaaca ctccgcccat gacgaacagc accagttctt gccgcaccgc taccgcgacg
      301 agccccagcg cggcaccgag tgccaaagca ccgatgtcgc ccatgaagac ctgcgcggga
      361 taggtgttga accacaagaa gcccaagccg gcgccagcta gggccgcaca gaaaactaaa
      421 agctcgccgg atcccgcgac gtacggaatc cccaaatacg aggagtagat gacgttgccg
      481 gccgcgtacg cgaagatgcc gagcgccgtg ccgaccagca cggacggcat gatcgcgagg
      541 ccgtcgaggc cgtcggtcag gttcacgccg ttgctcgcgc aaacgatcac gagcgccgtg
      601 aacacgacgt agccgaccgc gccgagcggg atcgtgattc ccggtatgta cgggatcagc
      661 agcgagtgct cgacggccgg gtcctcggcc gtcacgtaga acaggatcgc gagcccgaac
      721 gcgccgatcg tctgccagaa gagcttcgcg cgcacgctca agcccttcga gtttcgcaag
      781 acgactttct tgtagtcgtc gacgaagccg atgacgccga agtacagcgt gccgccgagc
      841 acgagcagca cgaatctgtt ctccaagtcg gcccagagca gagtgctgac cgcgatcgcg
      901 agcagtatca gcgcgccgcc catcgtcggc gtgcccgcct tcgggaagtg gctctcggga
      961 ccgacctggc gaatctgctg cccgatgcgc tgcatcgtga ggcgctggat caagtacggg
     1021 ccgaacgcga acgacagcac gagcgcagtc aacgcgccga gaatcgcgcg cgtcgtcagg
     1081 tagctgaaga cgttgaagcc cgagtagagc tccgtgaggt aatcggcgac gtagatcagc
     1141 atctcaggtc gtcctcggct cggcttcgag cgttttcacg agccggtcga gccccatgac
     1201 gcgcgagccc ttgacgagca cggtcacgtc gcccgcgagc agcggatcga gcgccgcgcg
     1261 agcggcctcg atatcggcca cggagcggcc atcgacgccg aacgcctttg ccgcttcccc
     1321 cgcgagatcg ccgatcgcgt agagcgcgtc gcagcggccg cgcgcgtact cgccgatctc
     1381 gcggtgcagc gccggacccg tggggccgag ctcggccatg ttgccgagca cgaggatgcg
     1441 cttgcccggg agcgccgcga gatgatcgat ggccgcgcgc accgagcccg ggttcgcgtt
     1501 gtaactgtcg tcgacgaccg tcgcgccgtt gcggccctgc acggcgcgca gccggccgcg
     1561 gacgggcgcg gcacgcgcga ggcccgcgac gatgtcttcg acgctcgtgc cgaccgcttg
     1621 cgcagccgcg gcggcggcga gtgcgttcgc gacgttctgg cggccgagca gcggcagcca
     1681 aacgtcggtc tcctcgccgt ccggcaaatg catgcggaac tcggagccgt tcgctaggaa
     1741 atggggcgac ccgagcacgc ggcagtccgc actcggcgcg aagccgaacg tcacagtgaa
     1801 ctcgcagcgc gaccgcgcga cccaatcgct gcggaactga tcgtcggcgt tcaacacggc
     1861 ggtaccggcg cgcggcaggt gatcgagcag ctcgcccttc gcgacgcata cgccgtgcag
     1921 cgagccgaaa ccctcgagat gcgccgctgc ggcgttcgtg atcacgccga cggtcggctg
     1981 cgccaagccc gcgagatagt cgatctcgcc ggcatgattg gcgccgagct cgatgacagc
     2041 cgcgtcgtgc tccgcggtca gcctcatcag cgtcaacggg acgccgatgt cgttgttcaa
     2101 gttgccctgc gtcacgcaga ccgagcggct gactccgagg atcgaggcaa tgagctcctt
     2161 gacggtcgtc ttgccgctgc tgccggtcac ggctacgacg ggaatcgtga acgacgcacg
     2221 ccaagcgcgc gccatcgcgc cgagcgcgcg gcgcgagtcc cggacttcga tctgcggcag
     2281 agccgacgcg gcgaggcgcg acacgagcgc gcccgccgcg cctttcgcga acgcctcgtc
     2341 gacgaagtcg ttgccgtcga agcggtcgcc cggaatcgcc acgaacagcg agccggaaac
     2401 gagcgcgcgc gtgtccgtcg tgaccgcgcc gaacgtgtga tccgcgccga cgaggcggcc
     2461 ttcgacctcg cgcgcgactg ctgcgagcgt gcgcggcatc atcgcgcgcc tccgagcagc
     2521 tcggcgacga cggcgcggtc gctgaatggc cggcgctcgt ggccgatgat ttgctccgcc
     2581 tcgtggcctt tgcccgcgac gagcacgacg tcgccgacgc gggcgcgctc gacggccgaa
     2641 gcgatcgcgg cacgccgatc gtggatgaca ctgacgcgcg ggtggtcgcc gacgccggca
     2701 cgaatgtcgt cgacaatgcc ggccggatcc tcgctgcgcg ggttgtcgtc ggtcagcacg
     2761 atgcggtcgg cgagatcggc ggcgacggcg cccatcatcg ggcgcttgcc gcgatcgcga
     2821 tcgccgccgc agccgaacac gcaccagagc tctccggtcg cggcgtcctc gagcgtcgtc
     2881 agcacgcgca gcaacgcgtc cggcgtgtgc gcgtagtcga cgacgaccca cgcgtgcccg
     2941 ggcgggccgc cgagcacttc catgcggccc ggcgccggct tcgccgccgc gagcgcggcg
     3001 caggccgccg gcaactccat gccctgcgcg agcagcgcgc cgagcgccga aaggagattc
     3061 tccgcgttga acgtgccgat cagcttcgag ggcaagcgcg cggtgccgaa cttcccggag
     3121 accgcaagct cgacaccggc caacgtcgag cgctcgagcc tagccgacag ctcgacggcc
     3181 gagctgcgca cgctcgtgcg caggagcgtg cagctcggcg ccaggccagc tgcgatcgtg
     3241 gccgagaacg cgtcgtcggc gttcagcacg gccaccttca ggttagggat agtgaacagc
     3301 ttgcgcttcg cctcgccgta gctcttgaga tcgccgtgct cgtcgaggtg atcgcgagtg
     3361 aggttcgtga acacggccgt gtggaactcg aggcccgcga tccgatcttg gctgagcgcg
     3421 tgcgacgaga cttccattgc ggcgcgcggc gcgccgagct ccgccagctc gcgatgcaga
     3481 gtcaggcaat ccggcgtcgt cagcgagtgc gccgtgagcg cgggcggcac gccgtaaccg
     3541 agcgtgccga catacgcgca cgggcgctgc ggttggctca agacttgcgc gagcaagtac
     3601 gcgaccgtcg tcttgccgtt cgtgcccgtc acgccgacga tcgtcggcgc ggcgccgggc
     3661 atgaagaacg cgcgcgccag ctcgccgagc cgcgacttca atcccggcac ggcgaggctc
     3721 ggccggggcg gctcgcccga atcttcgccg ggctcgtaca gaacgatcgc ggcccccgcg
     3781 tcgagcgcgg ctcgagcgta cttgatgccg tgctcgcggc cgccgcgcag cgcgacgaac
     3841 gcggcgccgg gccgcacggc gcggctgtcg agcgtcagat ccgtgatcgc gagacgcgag
     3901 tagctgccgg ccgcttttcc gagcaggtcg ccgagcgtgc gttcgcgtag ggcttgggcc
     3961 gccatcatgg cctcacctgc gcttgcgcaa ccaccgtcat ggccggcgcc ggcagcgcgt
     4021 cgggcggcac cgcgagcacg cgcagcgcgc cggaaacgat gttcgcgaac acgggggccg
     4081 cgatgtcgcc gccgtgatag gcgtctcctt gcggctcgtc gatcatgacg acgacgacga
     4141 gacgaggatc ggtcgcggga gcgaaaccgg cgaagatcgc gccgtggcgg tcgacgtcgt
     4201 aaccgccgac gcccgccttc tgcgtcgtgc cggtcttgcc ggccacgtgg taattgcgca
     4261 cggccgctcg ataacccgtg ccatccttcg cgacgacggc ctcgagcatc gaagtgaggt
     4321 cggcggcagt gtcggccgag atcacgcgct gccccggcgg cgcctcgtcg agcgccaata
     4381 acgagacggg ccgcaccacg ccgcccgccg caatcgcagc gtatgcgcgc gcgagctgca
     4441 acgtcgtgac cgtgaggcca tagccataag acagcgtcgc ctgccgcgca gtgctccaat
     4501 gctttgggtc cacgaggacg ccggcagatt cgccgggaaa agcgctatcg gtcaggcggc
     4561 cgataccgaa gcgtgtcagc acgtccgcga tcgcggcggg cttcatcgtg agcgcgacgc
     4621 gagcagcgcc gaggttgctc gacttgacga gcaccttcgt gagcgtgatg cgccccaggt
     4681 tttcgggatc cttgatgatg taactgtcga ccgtgatcgg gctcgaggta tcgataggcg
     4741 tgcgcgggcc gtaagacccg ctctcgagcg cgaccgccat gacgagcggt ttgaagctcg
     4801 agcccggctc gaaggggtcg gtaaccgcac gattgcggta gttgtcgggc cgatactccg
     4861 accgctcggc attcgggttc tgcgacg
//
LOCUS       DASSNN010000122         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_154175, whole genome shotgun sequence.
ACCESSION   DASSNN010000122 DASSNN010000000
VERSION     DASSNN010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154175"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFL84_02200"
     CDS             <1..284
                     /locus_tag="VFL84_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607461.1"
                     /translation="KQRDVKKVPRYHALPEWLVRGTDPVPLSEAFRSQATATRVQAVL
                     MSLIDGRRSIKDMVKLVVDQRLMTQEEAEPALRSFFINMHDEAKRGMTY"
     gene            complement(498..2414)
                     /locus_tag="VFL84_02205"
     CDS             complement(498..2414)
                     /locus_tag="VFL84_02205"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET7607462.1"
                     /translation="MLQKIHDSFGKAVIVVILGLISITFIFWGINGSDFSLSGTANFA
                     ARVNGEKIPLLEFERALQDRQNQYQQIYRTELSEDLRRELRRAVIEDLVQDAAIKQRV
                     EQQRYRASDERVIESVREIAAFQVDGQFSRDSYLALLANRGYTPTGFEALQREALEVG
                     DLQNGIADSSFLTPAELRRYIELYNQQREVAYASFDVEAFSDNIAVDDAAIASRYENN
                     QASYQTTETVDLEYVELALADIAAGLEVTEERLRQAYEEERARFETPEQRRARHILVT
                     VPEGQEDAARATAEGVVARLRGGEDFAAVAAEVSADAGTKAQGGELPWLARGVLPGPF
                     EDALFALEVGQISDPVLSDDGFHIIRLDEVRAGDVQPFEAVRDELAAETKTREAENEF
                     YDRANKLGEEAFDAYDDLAPVALATGLPIKTLMGFPRTGDSAAFDNSAAVVQAAFAEE
                     IVDSGRNTELVELADDHVLVLRVKQHHVPTTKPLDEVREQIREELVRERAQELAEEAA
                     RAFLADIEKGGDPAQSAAARGGTWNAAVWLQRTDDSVPTEVLSAAFGMPKTDAAAPQR
                     QVIALANGGQAVIVLTGVQPGEPEKLTEEEREQRRQQLEQQAARAELTGYAGNLRATA
                     DVRIPEDVLNPPVF"
BASE COUNT          375 a          850 c          841 g          441 t
ORIGIN      
        1 gcaagcagcg cgacgtcaaa aaggtcccgc gctatcacgc gttgccggag tggctggtgc
       61 gcggcaccga cccggtgccg ctcagcgagg catttcgcag ccaagcgact gcgactcgcg
      121 tccaggcggt cctgatgtcg ctgatcgacg gccgccgctc gatcaaggac atggtgaagc
      181 tcgtcgtgga ccagcgcctc atgacgcagg aggaagccga gcccgcgctg cgctccttct
      241 tcatcaacat gcacgacgag gcgaagcgcg gcatgaccta ctagccgtgg gcgtgtctca
      301 ctttgaccgc cgtgttgttg cgtgccaggc ggcggccgca cctcgatggc ccctcccgag
      361 agacgccgga agagcgtccc tgcggctcgt gttcgcacat ccatgtgctc acacgctctc
      421 gggaggggcc atcgaggtac agccgccgcc gtagaagctt ggtgggcggt caaagcgtgc
      481 cgaacggcgt cgggcgatca gaagaccggt gggttcagga cgtcttcggg gatccggacg
      541 tcggccgtcg ctcgcaggtt gccggcgtag ccggtcagct ccgctcgcgc ggcttgctgc
      601 tcgagctgct gccggcgctg ctcgcgctct tcctccgtca gcttctcagg ctcgcccggt
      661 tgcacgcccg tcagcacgat gaccgcttgg ccgccgttcg cgagcgcgat gacctggcgc
      721 tgcggcgcgg ccgcgtcggt tttcggcatg ccgaatgccg ccgagagcac ttcggtcgga
      781 accgagtcgt ccgtgcgctg cagccacacc gcggcgttcc acgtgccgcc gcgcgccgcg
      841 gcgctctgcg ccgggtcccc gcccttctcg atgtccgcca agaacgcgcg ggccgcttcc
      901 tcggcgagct cctgcgcgcg ctcgcgcacg agctcttcgc gaatctgctc gcggacctcg
      961 tcgagcggct tcgtcgtcgg aacgtgatgc tgcttgacgc gcagcacgag cacgtgatca
     1021 tccgcgagct cgacgagctc cgtgttgcgg ccgctgtcga cgatctcctc ggcgaacgcg
     1081 gcctgcacga cggccgcgct gttgtcgaag gcggccgagt cgcccgtgcg cgggaagccc
     1141 atcagcgtct tgatcggcag acccgtggcc aacgccacgg gcgcgagatc gtcgtaggcg
     1201 tcgaacgcct cctcgccgag cttgttcgcg cggtcgtaga actcgttctc ggcttcgcgc
     1261 gttttcgttt cggccgcgag ctcatcgcgc acggcctcga acggttgcac gtcgccggcg
     1321 cggacctcgt cgagccggat gatgtggaag ccgtcgtcgc tgagcacggg gtcgctgatt
     1381 tgcccgacct cgagcgcgaa cagcgcgtcc tcgaacggac ccggcaacac gccgcgcgcg
     1441 agccacggca gctcgccgcc ttgcgccttg gtgccggcgt cggccgagac ctcggccgcg
     1501 acggccgcga agtcctcgcc gccgcgcaag cgcgcgacca cgccctcggc cgtcgcgcgc
     1561 gccgcgtctt cctgcccttc cggcacggtg acgaggatat gccgggcgcg ccgctgctcg
     1621 ggcgtctcga agcgcgcgcg ctcctcctcg taggcctggc gcagccgctc ctcggtgacc
     1681 tcgaggccgg cggcgatgtc ggccaacgcg agctcgacgt actcgagatc gacggtctcg
     1741 gtggtctggt agctcgcttg gttgttctcg tagcgcgacg cgatcgccgc gtcgtcgacc
     1801 gcgatgttgt cgctgaacgc ttcgacgtcg aacgacgcgt acgcaacctc gcgttgctgg
     1861 ttgtagagct cgatgtagcg gcggagctcg gccggcgtca ggaacgacga atcggcgatg
     1921 ccgttttgca gatcgccgac ctcgagcgcc tcacgctgca gcgcctcgaa cccggtcggc
     1981 gtgtagccgc ggttcgcgag cagcgcgagg tagctgtcgc gcgagaactg accgtcgacc
     2041 tggaacgcgg caatctcgcg caccgattcg atcacgcgct cgtcggaggc gcggtagcgt
     2101 tgctgctcga cgcgctgctt gatcgccgcg tcttggacga gatcttcgat cacggcgcgc
     2161 cgcagctcgc ggcgcaggtc ctcggaaagc tccgtgcgat aaatctgctg atattgattc
     2221 tggcggtctt gcagcgctcg ttcgaactcg agcaacggga tcttctcgcc gttgactcgc
     2281 gccgcgaaat tcgcggttcc ggacagagag aagtcgcttc cgttgatgcc ccagaaaatg
     2341 aacgtgatgg agatcaagcc gaggatgacc acgataacgg ccttgccgaa ggagtcgtga
     2401 attttctgca acatcgttgg cgcccaacaa agaagggaca gatttatttt ccgggcgcgg
     2461 gcccgaaaaa caaatctgtc cctgtgaaat ggcggagtgg acgggac
//
LOCUS       DASSNN010000123         5932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1035288, whole genome shotgun sequence.
ACCESSION   DASSNN010000123 DASSNN010000000
VERSION     DASSNN010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035288"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="VFL84_02210"
     CDS             <1..1535
                     /locus_tag="VFL84_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HET7607463.1"
                     /translation="QIAFGAVIAALSPFKVALNPDVFFLLFLPPLLFLDGWRIPKEGL
                     LRDRGMILALAFGLVFITVLGMGLFIHWLIPAMPYAVAFALAAIVSPTDPVAVSAITA
                     RVPMPKRVMHMLEGESLLNDASGLVCFRFAVAAALTGMFSLSSAVLAFVQLAAVGVAL
                     GIGLTYGIVLAKNWISRRFGEESGSQVVITLLLPFVAYQAAEHFGGSGILAAVAAGVT
                     MSYAELSGGALASTRMQRTAVWDTVQFALNGIMFVLLGEQLPAIFSSAVRVVRESNHV
                     NPWWLAVYVLAINFGLAALRFGWVWLSMKGAALVARRRGAVPHEPTPRLVAAMSVAGV
                     RGAITLAGVLTLPLVSADGTPFPARDLAIFLAAGVIILSLTAANLALPRLLVGLAVPT
                     EHDGEAELDQARHAAAEAAIRAVEEALHEMARGHSDADFYAEAAARVMDRYRRRVEGH
                     ATGENAARIQHIDGIERRLRLAGLRAERDTIYALARAQKISDASSRKLVREVDLVEER
                     LR"
     gene            complement(1864..3504)
                     /locus_tag="VFL84_02215"
     CDS             complement(1864..3504)
                     /locus_tag="VFL84_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7607464.1"
                     /translation="MPDATRSTEEVPVLIVGGSLVGLTTALLLARHGVKALAVERHSG
                     TAIHPRAGHFQIRTIEILREMGLEDEGRRKARETYDPEGGIIAVESLAGKEVARFIPN
                     LNVGVDDRSPTGRLFLDQDVLEPMLRGTAEQLGARLLYRTEMTSFEQDADRVRAVIRN
                     LDSGEEREVVARYMVAADGNRSRVRKALGIEMHGYGLLSKSVTLYFEADCAPYLQGRN
                     SGVFYVHNPTLRGFFRLNRAGQRGFLAINVVGADVTREEAVNVSQGINEERALELLRA
                     AIGVPGLPMKVRQIVPWDAEANVASRFCEGRVFLAGDAAHVVPPNGGFGGNTGVQDAH
                     NLAWKIAMVLNGQAGSELLETYDMERRPVGAFTVDQAYGRYATRVVPERGKENLKPVV
                     DDLLIEIGYRYRSSAVLGGSADDPLVQHPDKLKAAPGTRAPHVVLERDGASISTLDLF
                     GEHFVLLTGPEGGAWRAAAEEAASALGVPIDVWQVGGERGLRDVTGRFLEAYALEPAG
                     AVLVRPDSFVAWRASDSASADGRTLGNALAAILSRRTA"
     gene            complement(3533..5029)
                     /locus_tag="VFL84_02220"
     CDS             complement(3533..5029)
                     /locus_tag="VFL84_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7607465.1"
                     /translation="MKKPIRALSAIAALMLISTQAHGQDARALLDGAMAAMGGARVEQ
                     LDTFVMTGFGQRYNFNGNISADPNSPPKWQAVATSERYFDLRGERALLRERAANMFPF
                     AAPFGMSLDVRERVQTGTALLDHPLAALRAALARPAQLGPVTVENGMAVVAFKLADGS
                     PGWIALDPQTHLPYWARWVNGSTTLGDVVNTSYFTGYLPVDGVQLPTGIMTKIDWRDQ
                     ATLMFQVDSYRLGVPALPPFPAANAAAPRRAAPEATTTKIADGVWDVRIANGGSGGAV
                     VELADRLVMFEPYGNEAELFARLDAANKLVRGKQVTAVVVSHHHDDHAGGLRAAVARG
                     LTIIAQRRTRALFEEWVSRPAVMFPDALAKNPRPLVFEPVDEHLVLEDSKHRLDIYHS
                     VGDFHMSDALIAYLPKEKILMEGDFTDDNWTWNWWAGAIKANRDLYGIDPQLDIPVHG
                     TPQSIEDKLAGAAKQAEAAQQFCVDNAARGSYPFGCPVRYSTTGLIEP"
     gene            complement(5070..>5932)
                     /locus_tag="VFL84_02225"
     CDS             complement(5070..>5932)
                     /locus_tag="VFL84_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7607466.1"
                     /translation="AREDLFTVVLEQFQTDTADYADYVLPATTQLEHTDVHKTYGHLY
                     MLANNPSIAPLGEALPNSEIFRRLAAKMGFDEPCFRESDDEIAAAAFTESSFDWQALK
                     KTGWQRLDRPKTYAPFANGGFPTKSGKCEIYSERLAELGMDPLPGYVPPHEVADESLA
                     RRYPLAMISPPARNYLNSTFVNVQSLRDAEGEPHLEIHPHDAAARDITDGARVRVFND
                     RGSLTLKARVTDRARPGVVVALSVWWKKLARDGKNANELTSQVLTDIGRAPTFYDCRV
                     ELEAAPLEPR"
BASE COUNT          844 a         2028 c         2040 g         1020 t
ORIGIN      
        1 tgcagatcgc cttcggcgca gtgatagccg cgctttcgcc gttcaaagtg gccctgaatc
       61 ccgacgtctt cttcttgctg ttcttgccgc cgctgctttt tctggacggt tggcgcattc
      121 cgaaagaagg actgctgcgc gatcgaggca tgattctcgc gctcgcgttc gggctcgtgt
      181 tcatcacggt gctcggcatg ggcttgttca tccattggct gattcccgcg atgccgtacg
      241 ccgttgcatt cgcgctcgca gccatcgtct cgcccaccga cccggtagcg gtgtcggcga
      301 tcaccgcgcg cgtgccgatg ccgaagcgcg tgatgcacat gctcgagggc gaatcgcttt
      361 tgaacgacgc ctcgggtctc gtctgcttcc gcttcgccgt ggccgcggcg ttaaccggga
      421 tgttctcgtt gtcgtccgcc gtcctcgcgt tcgtgcagct cgccgccgtc ggcgtcgcgc
      481 tcggcatcgg gctcacctac ggaatcgtct tggccaagaa ctggatcagc cgccgcttcg
      541 gcgaagaaag cggctcgcaa gtcgtgatca cgctgctgct cccgtttgtc gcgtatcagg
      601 cggccgagca ctttggtggc tccgggattc tcgcggccgt ggccgccggt gtcacgatga
      661 gctacgcgga gctttcgggc ggcgcgctcg cgagcacgcg aatgcagcgc acggcggttt
      721 gggacacggt gcagttcgcg ctgaacggca tcatgttcgt gctgctcggc gagcagctgc
      781 ccgcgatctt ctcgagcgcg gtacgcgtcg tgcgagagtc gaatcacgtg aacccgtggt
      841 ggctcgcggt ttacgtgctc gcgatcaact tcggcctggc ggcgttgcgt ttcggttggg
      901 tttggctatc gatgaagggt gccgcgctgg tggcgcggcg gcgtggcgcg gtgccgcacg
      961 agccgacgcc gcggctggtt gctgcgatgt ccgtggcggg cgtgcgcggc gcgatcacgc
     1021 tcgcgggcgt tctcacgctt ccgctcgtct cggccgacgg gacgccgttc cccgcgcgcg
     1081 accttgcgat ctttctcgcc gccggcgtca tcatcttgtc gctgacggcc gcgaacttgg
     1141 cgctgccgcg cttgctcgtc gggctcgccg tgccgaccga gcacgacgga gaagccgagc
     1201 tcgaccaggc gcgccacgcc gccgccgagg cggcgattcg cgccgtcgaa gaagcgctgc
     1261 acgagatggc gcgcggccac agcgacgcgg acttctacgc cgaagccgcg gcgcgcgtga
     1321 tggatcgcta ccggcgccgc gtcgaaggcc acgcgacggg cgagaacgcg gcgcgcatcc
     1381 agcacatcga cgggatcgaa cggcgtttgc ggctcgcggg gctgcgcgcc gagcgcgata
     1441 cgatctacgc tctcgcacgc gcccagaaga tatccgacgc gagctcgcgc aagctcgtgc
     1501 gcgaggtcga tctcgtcgaa gagcgcttgc gctgatgcat cgcagggcac tgtctaccct
     1561 ttcgagcgat tcggcggcac gaagccgcgc gtgaagcgcc gcgtatgcgc cgctcgcgag
     1621 catgtgcgga gtgcacgcag ggcgtgctcg tgagcgtgac cctactggag gcccttcgaa
     1681 gccacgtccc agctcgaatt cgtgcaaagc ccgcagctct tgccgacgaa tatcgcgccg
     1741 gccgtcttgt cgcctttcag ctgccatgcc gcccagctcc acgcgacgtt cgcgaactcg
     1801 ccgccgcccg ggtggaagaa cgtcgcgctg tgcccggcgc cgtggcgcgc gccgtacgct
     1861 cgctcaggcg gtgcggcgcg agaggatcgc cgcgagcgcg ttgccgagcg tgcggccgtc
     1921 ggccgacgcc gaatcgctcg cgcgccacgc gacgaacgag tccggccgca cgagcaccgc
     1981 gccggccggc tcgagcgcat acgcctcgag aaaccggccc gtgacgtcgc gaagaccccg
     2041 ctcgccgccg acttgccaaa cgtcgatcgg cacgccgagc gcgctcgcgg cttcctctgc
     2101 cgccgcgcgc caggcgccgc cttccggacc cgtgagcagc acgaaatgct cgccgaagag
     2161 atcgagcgtc gaaatcgatg cgccgtcccg ctcgagcacg acgtgcggcg cgcgcgtgcc
     2221 gggcgcggcc ttgagcttgt cggggtgctg caccaacggg tcgtcggccg agccgccgag
     2281 cacggcggac gagcgatagc ggtagccgat ctcgatcagc aggtcgtcga cgaccggctt
     2341 caggttctct ttcccgcgtt cgggcacgac gcgcgtcgcg tagcggccgt acgcctgatc
     2401 gaccgtgaac gcgccgacgg gccggcgctc catgtcgtag gtttcgagca gctcggagcc
     2461 cgcctgaccg ttcaggacca tcgcgatctt ccacgcgagg ttgtgggcgt cctgcacgcc
     2521 cgtgttgccg ccgaagccgc cgttcggcgg cacgacgtgc gcggcgtcgc ccgccaggaa
     2581 cacgcggccc tcgcagaagc gcgacgcgac gttcgcctcg gcgtcccacg gcacgatctg
     2641 ccgcaccttc atcggcagtc ccggcacgcc aatcgccgcg cgcaagagct cgagcgcgcg
     2701 ctcttcgttg atgccctgcg agacgttcac ggcctcctca cgcgtcacgt ccgcgcccac
     2761 gacgttgatc gcgaggaagc cgcgctggcc cgcgcggttg agccggaaga atccgcgcag
     2821 cgtcgggtta tgcacgtaga agacgcccga attgcggcct tgcaggtacg gcgcgcagtc
     2881 cgcttcgaaa tagagcgtca cgctcttcga aaggagcccg tagccgtgca tctcgatgcc
     2941 gagcgccttg cgcacgcggc tccggttgcc gtccgccgcg accatatacc gcgcgacgac
     3001 ttcacgttct tcgccgctgt cgagattgcg gatcacggcg cggacgcgat ccgcgtcctg
     3061 ctcgaacgac gtcatctcgg ttcggtagag gaggcgcgcg ccgagctgct cggccgtccc
     3121 gcgcagcatc ggctcgagca cgtcttgatc gaggaacagc cgtcccgtgg ggctcctgtc
     3181 gtccacgccc acgttgagat tcgggatgaa acgcgcgact tccttgcccg cgagggactc
     3241 gaccgcgatg atgccgccct ccgggtcgta cgtctcgcgc gctttgcgcc ggccctcgtc
     3301 ctcaaggccc atttcacgaa ggatctcgat cgtgcggatc tgaaagtggc ccgcgcgcgg
     3361 atggatcgcc gtgcccgagt gccgctccac ggcgagcgcc ttcacgccgt gccgcgcgag
     3421 caacagcgcg gtcgtgagcc cgaccaggct tcccccgacg atgagaaccg gaacctcttc
     3481 ggtcgagcgc gtagcatctg gcacgatgtt ttccctccta ttcggaggga gcttacggct
     3541 cgatgagccc cgtggtcgag tagcgcacgg ggcaaccgaa cggatagctc ccgcgcgccg
     3601 cattgtcgac gcagaactgc tgcgccgcct ccgcttgctt ggccgcgccc gcgagcttgt
     3661 cctcgatcga ttgcggcgtc ccgtgcacgg ggatgtcgag ctgcgggtcg atgccgtaaa
     3721 ggtcgcggtt cgccttgatc gcaccggccc accagttcca cgtccagttg tcgtccgtga
     3781 agtcgccttc catgaggatc ttttccttcg gcagataggc gatcagcgcg tccgacatgt
     3841 ggaagtcgcc caccgagtga tagatgtcga ggcggtgctt cgagtcctcg agcacgaggt
     3901 gctcgtccac gggctcgaac acgagcgggc gcggattctt cgcgagcgcg tcggggaaca
     3961 tcacggcggg ccgcgacacc cattcctcga acagcgcgcg cgtgcggcgc tgcgcgatga
     4021 tcgtgaggcc gcgcgccact gcggcgcgca ggccgcccgc gtgatcgtcg tgatgatggc
     4081 tcacgaccac ggccgtcacc tgcttgccgc gcacgagctt gttggccgcg tcgagacgcg
     4141 cgaatagctc ggcctcgttg ccgtacggct cgaacatcac gaggcgatcg gcgagctcga
     4201 cgacggcgcc cccgctgccg ccgttcgcga tccgcacgtc ccagacgccg tcggcgatct
     4261 tggtggtggt ggcctcgggc gccgcacgcc gcggggcggc cgcattggct gcggggaatg
     4321 gcggcagcgc cggcacgccg agccgatagg agtccacctg gaacatcagc gtggcctgat
     4381 cgcgccagtc gatcttggtc atgatgcccg tgggcagctg gacgccgtcg acgggcaggt
     4441 agcccgtgaa gtagctcgtg ttgacgacgt cgccgagcgt cgtgctgccg ttcacccagc
     4501 gcgcccagta gggcaggtgc gtctgcgggt cgagcgcgat ccagccgggg ctgccgtccg
     4561 cgagcttgaa cgcgaccacg gccatgccgt tctccaccgt gacggggccg agctgcgcgg
     4621 gccgcgcgag cgcggcgcgc agcgccgcca gaggatggtc gagtagcgcc gtgcccgttt
     4681 gcacgcgctc acgcacgtcc aacgacatgc cgaacggagc cgcgaacggg aacatgttgg
     4741 ccgcgcgctc gcgcaggagc gcgcgctcgc cgcgcaggtc gaaatagcgc tcgctcgtcg
     4801 cgacggcctg ccacttgggc ggcgagttcg gatcggcgct gatgttgccg ttgaagttgt
     4861 agcgctgacc gaatcccgtc atcacgaagg tgtcgagctg ctccacgcgc gcaccgccca
     4921 tcgcggccat ggccccatcg agcagcgcgc gcgcgtcctg gccgtgggcc tgcgttgaga
     4981 tgagcatgag cgcggcgatc gcagagagcg cgcgaatcgg ctttttcatg ggtcccccgg
     5041 acgtagttcg tgcgttcgag taacgcagct tagcgtggtt cgagcggcgc ggcctcgagc
     5101 tcgacgcgac agtcgtagaa cgtcggcgcg cggccgatgt cggtcagcac ctggctcgtg
     5161 agctcgttcg cgttcttgcc gtcgcgcgcg agcttcttcc accagaccga cagcgcgacg
     5221 acgacgccgg gacgcgcgcg gtccgtgacg cgcgctttga gcgtaagcga gccgcggtcg
     5281 ttgaagacgc ggacgcgcgc gccgtccgtg atgtcgcgcg cggcggcgtc gtgcggatgg
     5341 atctcgaggt gcggctcgcc ttccgcgtcg cgcaagcttt gcacgttcac gaacgtcgag
     5401 ttcaggtagt tgcgcgcggg cggcgagatc atcgcgagcg ggtagcggcg cgcgagcgac
     5461 tcgtcggcca cctcgtgcgg cggcacgtaa cccggcagcg ggtccatgcc gagctcggcg
     5521 agccgctccg aatagatctc gcacttgccg ctcttcgtcg ggaagccgcc gttcgcgaat
     5581 ggcgcatacg tcttcggccg gtcgaggcgt tgccagccgg tcttcttcag cgcctgccaa
     5641 tcgaagctcg attcggtgaa cgcggcggcc gcgatctcgt cgtcggactc gcggaagcac
     5701 ggctcgtcga accccatctt cgcggcgagc ctgcggaaga tctcgctatt cggcagcgct
     5761 tcgccgagcg gcgcgatcga cgggttgttc gcgagcatgt agaggtggcc gtaggtcttg
     5821 tgcacgtcgg tgtgctcgag ctgcgtcgtc gcgggcagca cgtagtctgc atagtccgcc
     5881 gtgtcggtct gaaactgctc gagcacgacg gtgaagaggt cctcgcgcgc ga
//
LOCUS       DASSNN010000124         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_265720, whole genome shotgun sequence.
ACCESSION   DASSNN010000124 DASSNN010000000
VERSION     DASSNN010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265720"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /gene="modB"
                     /locus_tag="VFL84_02230"
     CDS             complement(<1..595)
                     /gene="modB"
                     /locus_tag="VFL84_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HET7607467.1"
                     /translation="MPELELGPVWLSLQLATVTVLVLLLIATPLAWWLAFTRSRARTL
                     VEAVVAMPLVLPPTVLGFYLLVLLGPTGLIGAPWLSITDTTLTFSFAGLVLASVIYSF
                     PFTIQPLQAAFEGVGRAPLEAAATLGAGPLDRFWRVASPLALRGYVSAVVLTFAHTLG
                     EFGVVLMVGGNIPGSTKVISIAIYEHVETLEYGQAHIL"
     gene            complement(724..1236)
                     /locus_tag="VFL84_02235"
     CDS             complement(724..1236)
                     /locus_tag="VFL84_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HET7607468.1"
                     /translation="MNKRAKLELGFTLIELMIVVAIIGILAAIAIPAYQNYTIRAQVA
                     EGINLAAATKAAITTAFIDRGEAPMNRVEAGLSANATDSSGTYVTSVDINNGVLVVMY
                     GGSAHANISGRFVTLTPYESGDFSVVWRCGSAIEPPGLQELGTAAGGNAAVYLAPTVS
                     DQYLPATCRP"
     gene            complement(1422..2042)
                     /gene="folE"
                     /locus_tag="VFL84_02240"
     CDS             complement(1422..2042)
                     /gene="folE"
                     /locus_tag="VFL84_02240"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET7607469.1"
                     /translation="MPPRQRASTANKQNKPSRSEAEAAVRTLLRWIGDDPDREGLAGT
                     PARVVRAYEDWFSGYNDDPLEFLRRTFREVKGYDEMIVLRDISFESHCEHHMAPIIGK
                     VHIGYLPDRKVIGISKLARVVETFARRLQVQESLTAQIANCIQGVLKPRGVGVVVEGV
                     HQCMTTRGIRKHGVSMITSQLLGHFRSDPRTRAEFLTMIGRTGDRV"
     gene            2157..>2941
                     /locus_tag="VFL84_02245"
     CDS             2157..>2941
                     /locus_tag="VFL84_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET7607470.1"
                     /translation="MGARHLLQSFESWRDTGEPLVLATVYETAGSTYSKAGHRILLAA
                     NGDYRGLVSGGCLEGDLAERARGVIATAQAAAVTYDLRDSADDLWGLGVGCNGLLRVF
                     LQPLLPANDYQPFTAIAERLTGTRASGLATVIESGRSDLAVGATAVIGDSTEQIFNAD
                     AGGAALLRSAARIAMQTGTARFATEQGCRILSAPLHPIPRLLVLGAGLDAIPLVGMAA
                     ELGWFVTIADHRPAYVARGGFERADRAVLVEPGKLAASLALRD"
BASE COUNT          479 a          957 c          988 g          517 t
ORIGIN      
        1 agagaatgtg cgcttgtccg tattcgagcg tctcgacgtg ctcgtagatt gcgatcgaaa
       61 tcactttcgt gctgccgggg atgttgccgc ccaccatgag cacgacgccg aactcgccga
      121 gcgtgtgcgc gaacgtcaac accaccgcgg atacgtaacc gcgaagcgcg agcggcgacg
      181 cgacgcgcca gaagcgatcg agcggcccgg cgccgagcgt cgcggcggct tcgagcggcg
      241 cccgaccgac gccttcgaac gcggcttgca gcggctgtat ggtgaacggg aacgagtaga
      301 taacggatgc cagcaccagg cccgcgaacg aaaaggtcaa cgtggtgtcc gtgatgctga
      361 gccacggcgc gccgatgagg ccggtcgggc cgagcagcac gagcaagtaa aagccgagca
      421 ccgtcggcgg cagaacgagc ggcatcgcga cgacggcttc gacgagcgtg cgtgcgcggc
      481 tgcgcgtgaa cgcgagccac cacgcgagcg gcgtggcgat caagagtagg acgaggaccg
      541 tcacggttgc gagctgcagg ctgagccaaa cgggtccgag ctcgagttcc ggcatctcaa
      601 tcgtccgcga tcgtggccgg gccatgtagc cacgtgccgt agcggattgt caacgaagac
      661 agcagcgcgt cgaggcccgc gtgccgctcg cggcgcgcgt agcaatgtcg atgagcggtg
      721 ttactacggc cggcaggtcg ccggcaagta ctggtcggat accgtcggcg ctagatagac
      781 ggccgcgttg ccgccggctg ccgtgccgag ctcttggagc ccgggcggct cgatcgcgct
      841 gccgcagcgc caaacgacgc taaaatcgcc cgattcgtac ggcgtcagcg tgacgaagcg
      901 gccgctgatg ttggcgtgcg cactaccgcc gtacatgacg acgagcacgc cgttgttgat
      961 gtcgaccgac gtcacgtatg tgccactcga atcggttgca ttcgcggata gccccgcctc
     1021 gacgcggttc atgggcgcct cgccgcggtc gatgaacgcc gtcgtgatgg cggctttcgt
     1081 ggccgcggcg agattgatgc cctcggcgac ttgcgcgcga atcgtgtagt tctgatacgc
     1141 cggaatcgcg atggcggcga ggattccgat gatcgcgacg acgatcatca gctcgatcag
     1201 cgtgaatccc agctctagct ttgctcgctt gttcatttag ttttgcctgt gcgcggctag
     1261 gcgctcgcgc gccgtggtgt cgaggttctg ctcgaatatc gagcaagacg tgtgccagcg
     1321 acggtgaacg gccgctttgc gggcgccgcg accgctttat gtccatcggc agtgacgaag
     1381 gtggtcagct ttggacgtct catccgatta tgtcgcggcc gtcagacgcg gtcgcccgtt
     1441 ctgccgatca tcgtcaagaa ttccgcgcgc gtgcgcggat ccgagcggaa gtggccgagc
     1501 agctggctcg tgatcatcga tacgccgtgc ttgcggatgc cgcgcgtcgt catgcactga
     1561 tgcacgcctt cgacgacgac gccgacgccg cgcggcttca acactccctg aatgcaattc
     1621 gcgatctgcg ccgtcaacga ttcctgcact tgcagccgcc gcgcgaacgt ctcgacgacg
     1681 cgcgcgagct tactgatacc gatgactttg cgatcgggga gatagccgat gtgcaccttg
     1741 ccgatgatcg gcgccatgtg atgctcgcag tgcgactcga agctgatgtc gcgcagcacg
     1801 atcatctcgt cgtagccctt gacctcacgg aacgtgcggc gcaggaactc gagcggatcg
     1861 tcgttgtagc cgctgaacca gtcttcgtaa gcccggacga cgcgggccgg tgttccagcg
     1921 agcccttcgc ggtccggatc gtcgccgatc caccgaagga gtgtacggac ggcggcttcc
     1981 gcttcgctgc gagagggctt gttttgtttg ttggccgtgg aagctcgttg gcggggcggc
     2041 acagcgttcc tctcgttcgc gaagtcggtc gggcagtcta agtctgcgct cgtaggcggt
     2101 gcaaccgagc agaggcgctg gcatattgac gagccaacat tgtttcagag cgaagcatgg
     2161 gagcacgtca tctactgcag tcgttcgaga gctggcgcga caccggcgag ccgctcgtgc
     2221 tcgcgacggt ctacgagacc gccggctcga cctacagcaa ggccgggcac cggatcctgc
     2281 tcgcggcgaa cggcgattat cgcggcctcg tcagcggcgg ctgcctcgaa ggcgatctcg
     2341 ccgagcgcgc gcgcggcgtc atcgcgacgg cgcaggcggc ggccgtgacg tacgacctgc
     2401 gcgattccgc cgacgatctc tggggactcg gcgtcggctg caacggcttg ctgcgcgtgt
     2461 tccttcagcc gctgctcccc gccaacgact atcagccctt caccgcgatc gccgagcgcc
     2521 tcaccggcac gcgtgcgagc ggcctcgcga cagtcatcga gagcggccgc tcggatctcg
     2581 ccgtcggcgc caccgccgtc atcggcgact cgacggagca gatcttcaac gccgatgccg
     2641 gcggagccgc gctgcttcgt tcggccgctc gcatcgcgat gcagaccggc acggcgcggt
     2701 ttgcgacaga gcagggctgc cgcatcctct ctgcgccgtt gcatccgatt ccgcgcttgc
     2761 tcgtgctcgg cgccggcctc gacgccattc cgctcgtcgg catggcggcc gagctcggct
     2821 ggttcgtcac gatcgccgac caccgcccgg cctacgtcgc gcgcggcggc ttcgagcgcg
     2881 ccgaccgtgc cgtcttggtc gagcccggca agctcgccgc atccctggca ctgcgcgact
     2941 t
//
LOCUS       DASSNN010000125         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1162052, whole genome shotgun sequence.
ACCESSION   DASSNN010000125 DASSNN010000000
VERSION     DASSNN010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162052"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..172)
                     /locus_tag="VFL84_02250"
     CDS             complement(8..172)
                     /locus_tag="VFL84_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HET7607471.1"
                     /translation="MKKYMCLICGFIYDEAEGRPDEGIPPGTKWADIPLSWRCPDCGS
                     GKEDFEMVEV"
     gene            complement(220..810)
                     /locus_tag="VFL84_02255"
     CDS             complement(220..810)
                     /locus_tag="VFL84_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET7607472.1"
                     /translation="MSELPLFPLQTVLLPDGLLALRVFEARYVDMVARCLRGENRFGV
                     VAIREGAEVGAATTYDHGTSAEIVDWLEEQRGLLGILAVGREPFRLLETRREPDGLYV
                     GSVEWLERMPAQKLLPKHEPLAALLKRLIEPLTLYRGAATSFDDAAWVGARLAELLPL
                     AIEFKQSLLEMRDAEARLDRLAAALEPPEGGPDPGS"
     gene            complement(840..>2099)
                     /gene="mpl"
                     /locus_tag="VFL84_02260"
     CDS             complement(840..>2099)
                     /gene="mpl"
                     /locus_tag="VFL84_02260"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HET7607473.1"
                     /translation="STQLESLGIELVDGYEAPIDPGVESVVVGNALSRGKPVVEALLN
                     SGRAYTSGPEWLADHVLRDKWVLAVSGTHGKTTTTSMLAWILEYAGLKPGFLIGGVPG
                     NFGISARLGGSKYFVVEADEYDTAFFDKRSKFVHYRPRTLVINNIEYDHADIFSDVDA
                     IIWQFHQLLRTVPGNGLIVANGKDANIEKLLKRGVWTPLATFSASDRAARWFGAYDSI
                     GTESRFTVFEQGAARGSGRWSLLGQHNLENALAAIAAAGHVGVAPETALEALAQFKGV
                     KRRLELRGTFAGITLFEDFAHHPTAIETTLQGVRSLARQQRIVAVMEPRSNTMRMGVH
                     RDTLHGSFAAADRVFVLAPKDLGFDPAQALAPLGERLVVAADVQALLERLLGELRAGD
                     HVVLMSNGSFQGLPASLERALASRKPS"
BASE COUNT          333 a          721 c          694 g          351 t
ORIGIN      
        1 cggcacctca gacctccacc atctcgaagt cttccttgcc cgagccgcaa tccgggcaac
       61 gccacgacag cggaatgtcg gcccacttcg tgcccggagg aattccttcg tccgggcgtc
      121 cttcggcttc gtcgtagatg aagccgcaga tcagacacat gtacttcttc attcgttatc
      181 cggcgcttcc gttggcgtct cttggcgcgc aaaagcgtat cacgagcccg gatctggccc
      241 gccttccggc ggctcgagcg cggccgcgag ccggtcgaga cgcgcttcgg cgtcgcgcat
      301 ttcgagcagc gactgcttga attcgatcgc gagcggcagg agctcggcga gtcgcgcgcc
      361 gacccacgcg gcgtcgtcga acgaggtcgc agcgccgcga tacagcgtca gcggctcgat
      421 gagccgcttc agcagcgccg cgagcggctc gtgcttggga aggagcttct gcgccggcat
      481 tctctcgagc cattcgacgc tgccgacata gaggccgtcc ggctcgcgcc gcgtttcgag
      541 caagcgaaac ggctcgcgcc cgacggcgag gatgccgagc aggccgcgct gctcctcgag
      601 ccagtcgacg atttcggcgc tcgtgccatg gtcgtaagtc gtcgcggcgc cgacctcggc
      661 gccctcgcgg atcgcgacga cgccgaaccg gttctcgccg cgaaggcagc gcgcgaccat
      721 gtcgacgtag cgggcctcga acactcgcag cgcgagcaag ccgtcgggca gtagcacggt
      781 ctgcagcggg aacagcggca gctcgctcat cgtgggttgc gcgccgcttg tttcggcctt
      841 cagctcggct tgcggctcgc gagcgcgcgc tcgagcgagg ccggcaagcc ctgaaaactg
      901 ccgttcgaca tcagcacgac gtggtcgccg gcgcgaagct cgccgagcag ccgctcgagc
      961 aacgcttgca cgtcggctgc gacgacgagc ctctcgccga gcggcgccag cgcctgcgcc
     1021 ggatcgaacc ccaagtcctt gggcgcgagc acgaacactc tgtcggcagc agcgaacgag
     1081 ccgtgcagcg tgtcgcggtg cacgcccatg cgcatcgtgt tcgagcgcgg ctccatgacc
     1141 gcgacgatgc gttgctgccg cgcgaggctg cgcacgcctt gcagcgtcgt ctcgatagcc
     1201 gtcggatgat gcgcgaaatc ctcgaacaac gtgattccgg cgaatgtgcc gcgcagctcg
     1261 agccggcgct tgacgccctt gaactgcgcg agcgcttcga gcgccgtctc gggtgcgacg
     1321 ccgacgtggc cggccgccgc gatcgccgcg agcgcgttct cgaggttgtg ctggccgagc
     1381 agcgaccagc gaccgctgcc gcgcgccgca ccctgctcga agaccgtgaa gcgcgattcc
     1441 gtgccgatcg agtcgtatgc gccgaaccag cgcgcggcgc ggtcgcttgc gctgaacgtc
     1501 gcgagcgggg tccagactcc gcgcttcagc agcttctcga tgttcgcatc cttgccgttc
     1561 gcgactatga ggccgttgcc cggcaccgtg cgcagcagct ggtgaaactg ccaaatgatc
     1621 gcgtcgacgt cgctgaaaat gtcggcgtga tcgtactcga tgttgttgat tacgagcgtg
     1681 cgcgggcggt agtgcacgaa tttcgagcgc ttgtcgaaga aggccgtgtc gtactcgtcg
     1741 gcttcgacga cgaaatactt cgagccgccg agccgcgccg agatgccgaa gttgccgggc
     1801 actccgccga tcagaaagcc cggcttcaag cccgcgtact cgaggatcca cgcgagcatg
     1861 ctcgtcgtcg tcgtcttgcc gtgcgttccc gagacggcga gcacccactt gtcgcggagc
     1921 acgtggtccg cgagccattc gggacccgac gtgtacgcac ggccggaatt cagcagcgcc
     1981 tccacgacgg gcttgccccg cgacagcgca ttgccgacga cgacgctctc gacgccgggg
     2041 tcgatcggtg cttcgtagcc gtcgacgagc tcgatgccca gagactcgag ctgcgtgct
//
LOCUS       DASSNN010000126         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1413802, whole genome shotgun sequence.
ACCESSION   DASSNN010000126 DASSNN010000000
VERSION     DASSNN010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413802"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..613
                     /locus_tag="VFL84_02265"
     CDS             104..613
                     /locus_tag="VFL84_02265"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET7607474.1"
                     /translation="MSSATLVLVAGAALAVTLSAASQSAEQAFVKEAVQSNIAEVQLG
                     ELAAQRAEMDAVRKFGETLRVDHQALLQRATNLAKSLKVEAPTEPSVEARGIYKGLAQ
                     LSGSEFDAAFVSHMITFHEATIATYSRNANSDDDGVATMVADALPKLKAHLATAQALQ
                     KGAPAHAQH"
     gene            complement(620..1939)
                     /locus_tag="VFL84_02270"
     CDS             complement(620..1939)
                     /locus_tag="VFL84_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HET7607475.1"
                     /translation="MRSYTRWLVGVLGVCALPLADGCGGSAQLTVEDGMGPDPKLPPP
                     KKSLIPVIDIAPAVGWTDGGAPVAADGLTVTAYARGFDHPRWLYVLPNGDVLVAETAA
                     PERESARQGVKGLVMAKVQQNAGSAVPSADRITLLRDRDRDGQPELRTAFIENLHSPF
                     GMALVGDDLYVANTDALMKFDYRRDATTLTDPGTRVTELPAGAVNYHWTKNVVASRDG
                     SRLYVTVGSNSNVAERGIEQERERAAILEVDPASGATSVYASGLRNPNGLAWEPRTGA
                     LWTVVNERDEIGSDLVPDYLTAVDRGAFYGWPYSYYGSHVDPRVEPPNPELVASARVP
                     DYGLGPHTASLGLVFYGADELEDYRGGAFVAQHGSWNRKPLSGYRVIYVPFSAGRPRG
                     EAREVLTGFVNDEGEARGRPVGLAVDAMGALLVADDVGNTIWRVTKR"
BASE COUNT          362 a          712 c          705 g          313 t
ORIGIN      
        1 ggcgagctcg tcgataggct cggcatcgga gtcagcgacg gcgcaaatgc cgacgcgcct
       61 agctcgttac ggcacaggac gacttgagtc gaggagaatt ccgatgtcgt cagcaacact
      121 cgttttggtc gccggcgcag cgctggccgt gacgctctcg gcggcgagcc agagcgccga
      181 gcaggccttc gtgaaagagg ccgtgcagag caacatcgcc gaggtgcagc taggcgagct
      241 cgccgcgcag agagcggaga tggacgccgt gcgcaaattc ggcgagacgc tgcgcgtcga
      301 tcaccaggct ttgctgcagc gcgcgacgaa ccttgcgaaa tcgctgaagg tcgaggcgcc
      361 gacggagccg tccgtggagg cgcggggcat ctacaaggga ctcgcgcagc tttcgggcag
      421 cgagttcgac gccgccttcg tgagccacat gatcaccttc cacgaagcca cgatcgcgac
      481 gtatagccgc aacgcaaaca gcgacgacga cggggtcgcg acgatggtcg cggacgcgtt
      541 gccgaagctg aaagcccacc tcgcgacggc gcaggcgctg cagaaagggg ccccggctca
      601 cgcgcagcac tgaagcgctt cagcgcttcg tgacgcgcca aatcgtgttg ccgacgtcgt
      661 cggcgacgag cagcgcgccc atcgcatcga ccgccaagcc gacgggccgg ccacgggcct
      721 cgccttcgtc gttgacgaac cccgtgagca cctcgcgcgc ctcgcctcgc gggcgacccg
      781 ccgagaacgg cacgtagatc acgcgataac ccgataaggg cttgcggttc cacgagccgt
      841 gctgcgccac gaatgcgccg ccgcgataat cctcgagctc gtcggcgccg taaaacacga
      901 ggccgagcga agccgtgtgc gggccgagac cgtaatccgg cacgcgcgcc gaggcgacga
      961 gctcggggtt cggcggctcg acgcgcgggt ccacatgact gccgtagtag ctgtacggcc
     1021 aaccgtagaa cgctccgcgg tcgacggccg tcagatagtc cggcacgaga tcgctgccga
     1081 tctcgtcgcg ctcgttgacg acggtccaca acgcgccggt ccgcggctcc cacgcgaggc
     1141 cgtttggatt gcgcaagcca gacgcataga cgctcgtcgc gccgctcgcg gggtcgacct
     1201 cgagaatcgc agcgcgctcg cgttcttgct cgatgccgcg ctcggcgacg ttgctgttcg
     1261 agccgacggt cacatagagg cgcgagccgt cgcggctcgc gacgacgttc ttcgtccagt
     1321 gatagttgac ggcgccggcc ggcagctcgg tcacgcgcgt gccgggatcc gtcaacgtcg
     1381 tcgcatcgcg ccgataatcg aacttcatca gcgcgtccgt gttcgcgacg tacaagtcgt
     1441 cgccgacgag cgccatgccg aacggcgagt gcagattttc gatgaacgcc gtgcgcagct
     1501 caggctggcc gtcgcgatcc ctgtcgcgca gcaacgtgat gcggtcggcg ctcggcaccg
     1561 cggagccggc gttttgctgc accttcgcca tcacgagacc tttgacgccc tggcgggcgc
     1621 tctcgcgctc gggcgccgcg gtctcggcga cgagcacgtc gccgttcggc agcacgtaaa
     1681 gccagcgcgg atggtcgaag ccgcgcgcgt acgccgttac ggtgaggccg tcggccgcga
     1741 ctggcgcacc gccgtcggtc cagccgaccg ccggcgcgat gtcgatgacc ggaatcaggc
     1801 tcttcttcgg cggcggcagc ttggggtccg gacccatgcc gtcctcaacc gtcagctgcg
     1861 cggacccgcc gcacccgtca gccagcggca aggcacatac gcccaagacc ccgacaagcc
     1921 atcgcgtgta gctgcgcatc gagctctcct cgagcccaat cagcaagttg catgccattc
     1981 gctgcagttt ctgcacgcga acgcaaagat tgcgcggcgc gcgcgcgagg gcgcgcggtt
     2041 gcggaggcgt gtagttggta cgtggcttgc aacgaacccc gaaagtcacg cg
//
LOCUS       DASSNN010000127         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_265787, whole genome shotgun sequence.
ACCESSION   DASSNN010000127 DASSNN010000000
VERSION     DASSNN010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265787"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFL84_02275"
     CDS             <1..535
                     /locus_tag="VFL84_02275"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET7607476.1"
                     /translation="VGGSALDHLSALQADAPDYRGLAEEWEAFRQASVLAIESSINSG
                     DWAAADMQLAGLAKAPAGALAAQPLAAELAARRLQASYLATAAQASTMTLQSGAQAVY
                     PPELLARAVEGWVDLEFVVDRDGRTRDVVVKQASPPDRFDEAAVTAVQQYRYVPFERD
                     GRTYERRLKMRMRFQVQ"
     gene            complement(593..1570)
                     /locus_tag="VFL84_02280"
     CDS             complement(593..1570)
                     /locus_tag="VFL84_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HET7607477.1"
                     /translation="MRTKTVLASALAALLATGAAFAQAPAQQPAQPQPFRAGTPLSQT
                     NEAGQLKTMSSNVKVYGSFHFSESCAYDPDRNLIVAINAANPRDMGGPPPPLTPNDGF
                     VSLINPDGSVHTSKWIGVTRDGLTLVHPLGSAVQGGTLYVNDGNYIRTFDLKTGAPKQ
                     SFEVKESLNLNGIGVAPDGTMYASNTGNPQRIYKVTPAGVSSVFVDGPPLLQPNGVAI
                     DPQGNVVVVNIGNNDVLTFDTAGKLVKTEHAKEAGNDGLVILPDGTKYVSSVRFGSVS
                     KIAPGKEAEVIAEGIPSAASMCYDSKQNQLVIPLNPNNALAFIPLGRRR"
     gene            complement(1642..2043)
                     /locus_tag="VFL84_02285"
     CDS             complement(1642..2043)
                     /locus_tag="VFL84_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET7607478.1"
                     /translation="MKTVVRTASPIAFIDKVKNEIKRKDSHELVAMMQDITGEPPKMW
                     GTSIVGFGRREYRYASGRSGEICLMGFSPRSGAIVVYLGPGIENDKLMGKLGKHKRGK
                     GCLYINKLDDVDRGVLRELVEHSIAELRKPA"
     gene            2166..>2628
                     /locus_tag="VFL84_02290"
     CDS             2166..>2628
                     /locus_tag="VFL84_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET7607479.1"
                     /translation="MKTRTFAAGCAAAFGTMLATLLFAAEQATYPVTKKIDHVDDYHG
                     TKVADPYRWLEDETSPETAAWVEAQNAVTFPYLERIPYRDALRARVMELNDYERYGAP
                     ARKGPYFFFNKNEGLQNQSVLYIQKGLDGAPEVLLDPNSWSADGTVQLSAFE"
BASE COUNT          426 a          823 c          906 g          473 t
ORIGIN      
        1 ggtcggaggc agcgcgctcg atcatttgag cgccttgcag gccgacgcgc ccgactaccg
       61 cgggctcgcc gaggagtggg aagcgtttcg acaagcctcg gtgctcgcga tcgaaagctc
      121 gatcaacagc ggcgactggg ccgcggcgga tatgcagctc gccgggctcg cgaaagcgcc
      181 ggccggcgcg cttgcggcgc agccgctcgc ggccgagctc gcggcgcggc ggctgcaagc
      241 gagctacctc gcgaccgccg ctcaagcgag cacgatgacg ctgcagagcg gcgcgcaggc
      301 ggtctatccg ccggagctgc tcgcccgcgc ggtcgagggc tgggtcgatc tcgagttcgt
      361 cgtcgatcgc gacggtcgta cgcgcgacgt cgtcgtcaag caggcgtcgc cgcccgaccg
      421 cttcgacgag gccgcggtta cggccgtgca gcagtaccgc tacgtgccgt tcgagcgtga
      481 cggccgaacc tacgagcggc gcctcaagat gcgcatgcgc ttccaggtgc agtagccgag
      541 tagagcaggg gccgcggctg cgaaagccgc ggccccagcc aacgtcacgt gcctagcgcc
      601 ggcggccgag cgggatgaac gcgagcgcgt tgttcgggtt cagcggaatc acgagctggt
      661 tctgcttcga gtcgtagcac atcgacgcgg cggacgggat gccttcggcg atgacctcgg
      721 cttccttgcc cggcgcgatc ttcgaaacgc tgccgaagcg tacgctgctc acgtacttcg
      781 tgccgtcggg caatatgacg aggccgtcgt tgcccgcttc cttggcgtgc tcggtcttga
      841 cgagcttgcc cgccgtgtcg aacgtcagga cgtcgttgtt gccgatgttg acgacgacga
      901 cgttcccttg cgggtcgatc gcaacgccgt tcggctgcag cagcggcggg ccgtcgacga
      961 acacggacga tacgccggcc ggcgtgactt tgtagatgcg ctgcgggttg cccgtgttcg
     1021 acgcgtacat cgtgccgtcc ggcgcgacgc cgatgccgtt caagttcaga gattccttga
     1081 cctcgaacga ttgcttcggc gctcccgtct tcaagtcgaa cgtgcggatg tagttgccgt
     1141 cgttgacgta gagcgtgccg ccctggaccg cgctgccgag cggatgcacg agtgtcaagc
     1201 cgtcgcgcgt gacgccgatc cacttggacg tgtgcaccga gccgtcgggg ttgatcagcg
     1261 atacgaagcc gtcgttcggc gtgagcggcg gcggaggacc gcccatgtcg cgaggattgg
     1321 cggcgttgat ggcgacgatc aggtttcggt cgggatcgta cgcgcagctc tcggagaaat
     1381 gaaagctgcc gtagaccttg acgttgctcg acatcgtttt cagttgcccg gcttcgttcg
     1441 tctggctgag cggcgtgccg gcgcgaaacg gttgcggctg cgcgggctgc tgagccggcg
     1501 cctgagcgaa tgcggcgccg gtcgcgagca gtgcggcgag agcggaagcc aaaacggttt
     1561 tggtgcgcac gagatccccc agggatgttg ttgttggcga aggaaacatg atggccgatc
     1621 gcgcagcggc ttgaaagccg gtcaagcggg cttgcgcagc tcggcgatcg aatgctcgac
     1681 gagctcgcgc agcacgccgc gatccacgtc gtcgagcttg ttgatgtaga ggcaaccctt
     1741 gccgcgcttg tgcttgccga gctttcccat gagcttgtcg ttctcgattc cggggccgag
     1801 atagacgacg atcgcgcccg agcgcggcga gaatcccatc aagcagatct cgcccgagcg
     1861 gccgctcgca taccggtatt cgcggcgccc gaacccgacg atcgacgttc cccacatctt
     1921 cggcggctcg cccgtgatgt cctgcatcat cgcgacgagc tcgtggctgt ccttgcgctt
     1981 gatctcgttc ttgaccttgt cgatgaacgc gatcggactg gcggtgcgga cgacggtttt
     2041 catttttcgt ttggtcgcgg ccatcgcata gggctccgga attctggagg cattgtggcg
     2101 tagcgccggg gtggacgcga gcggcataat ccttaggagt tgaacgagcc tcgagaggcg
     2161 atcccatgaa gactcgcacg ttcgccgccg ggtgcgcggc ggccttcggg acgatgctcg
     2221 cgacgttgtt gttcgcggcg gagcaggcga cctatcccgt gacgaagaaa atcgaccatg
     2281 tagacgacta tcacggcacg aaagtcgccg acccgtaccg ctggctcgag gacgagacgt
     2341 cgcccgagac ggcggcgtgg gtcgaggcgc agaacgccgt gacgttcccg tatctcgagc
     2401 gcattccgta ccgcgacgcg ctgcgcgcgc gcgtcatgga gctcaacgac tacgagcgct
     2461 acggcgcgcc ggcgcgcaag ggcccgtact tcttcttcaa caagaacgaa ggcctgcaaa
     2521 accagagcgt gctgtacatc cagaagggcc tcgacggcgc gcccgaggtg ctgctcgatc
     2581 cgaactcgtg gtctgccgat ggcacggtgc aactctcggc cttcgagc
//
LOCUS       DASSNN010000128         2763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1162155, whole genome shotgun sequence.
ACCESSION   DASSNN010000128 DASSNN010000000
VERSION     DASSNN010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162155"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..498)
                     /gene="greB"
                     /locus_tag="VFL84_02295"
     CDS             complement(7..498)
                     /gene="greB"
                     /locus_tag="VFL84_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HET7607480.1"
                     /translation="MGRYRAPRPPSAKYITPEGAARLRRELDELWRVKRPAVTRAVAE
                     AAAQGDRSENAEYIYGKKQLAEIDRRVRFLRKRLTGIRVVDQLPSDTSKVFFGAWFEL
                     TSRDTKREYRLVGPDELDLAPDYISVDSPLGRAVLGKGLGAEIVVRTPEGESHYELTA
                     VRY"
     gene            562..1554
                     /gene="lipA"
                     /locus_tag="VFL84_02300"
     CDS             562..1554
                     /gene="lipA"
                     /locus_tag="VFL84_02300"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET7607481.1"
                     /translation="MSEFRGIPVVTTGTKYQTEQGFAAIKNGVKARADRQQAPREPKP
                     KWLKARVPSGERFERLRATVREHRLATVCEESICPNIGECWNNGTATIMLMGKVCTRA
                     CQFCAVDTGNPHGWLDTHEVANSARTVELMGLKYVVLTSVDRDDLPDGGARHYADCIR
                     AIKARCPDTAVEALTPDFAGKLADVATVVDSEVDVFAQNLETVERLTRKVRDARAGYR
                     QTLDVLAFAKRHRPQVLTKSSLMLGLGETDQEIVAALDDLKRAAVDIVTFGQYLRPTP
                     NHLAVERYVTPEQFERYREQGLERGFLEVVAGPLVRSSYRADRVFEKNNVGLSG"
     gene            1555..2532
                     /gene="sohB"
                     /locus_tag="VFL84_02305"
     CDS             1555..2532
                     /gene="sohB"
                     /locus_tag="VFL84_02305"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HET7607482.1"
                     /translation="MSEFLAAYGLFLAELATVALVLVIVVVLIASSRRHGHKEGLVVE
                     HLNRRFEDSADNLKLAIEGKGRYKKALKARLKERKREDKARSKDGKPRLFVLDFKGDL
                     RATAAASLREEVSAVLRVARPGEQVLVRLENSGGTVHEHGFAASQLTRLKEHGLKLIV
                     AVDKVAASGGYLMACVADRLIAAPFAIVGSIGVLAQLPNFHRLLEQKGVDFEQVTAGR
                     YKRTLTMFGENTDEGRAKLKEQVEEIHALFKAQIREHRPQVDVESVATGEHWHGVRAL
                     ALKLVDELKTSDDVLLEAAKDHDVYHLTYKRRRTLQERILGGAESLLSR"
BASE COUNT          478 a          908 c          934 g          443 t
ORIGIN      
        1 cggcacctag tagcggacgg cggtgagctc gtagtggctt tcgccttcgg gcgtgcgcac
       61 gacgatctcg gcgcccaagc ctttaccgag aacggcgcgg ccgagcggtg agtcgacgct
      121 gatgtagtcg ggcgcgagat cgagctcgtc ggggccgacg agccggtatt cgcgtttcgt
      181 gtcgcgcgac gtgagctcga accacgctcc gaaaaagacc ttgctcgtat cgctcggcaa
      241 ctgatcgacg acgcgaatgc cggtcaagcg cttgcgcaag aagcgaacgc gccggtcgat
      301 ctcggcgagc tgcttcttgc cgtagatgta ctccgcgttc tcggaccggt cgccctgcgc
      361 cgctgcttcg gcgacggcgc gtgtcacggc ggggcgcttc acgcgccata gctcgtcgag
      421 ctcgcgcctg agccgcgcgg ccccttcggg cgttatgtat ttggcgcttg gcggacgcgg
      481 cgcccgatag cggcccataa caccccattc ccttggcatg cgatactcga tcgccacatt
      541 cctcgaatgt taccaagcac gatgagcgaa tttcgcggca tccccgtcgt gacgaccggt
      601 acgaagtacc aaacggagca ggggttcgcc gccatcaaga acggtgtgaa ggcgcgagcg
      661 gatcgtcagc aggccccgcg cgagccgaag ccgaagtggc tgaaagcgcg cgttccgagc
      721 ggcgagcgat tcgagcggct gcgcgcgacc gtgcgcgagc atcgtctcgc gacggtctgc
      781 gaggagtcga tctgccccaa catcggcgag tgctggaaca acggcaccgc gacgatcatg
      841 ttgatgggca aggtctgcac gcgcgcgtgc cagttctgcg ccgtcgacac cggcaaccca
      901 cacggctggc tcgacacgca cgaggtcgcg aactcggcgc gcacggtcga gctcatgggc
      961 ctcaagtacg tagtgctgac gtccgtcgac cgcgacgact tgcccgacgg cggcgcgcgc
     1021 cactatgccg attgcatccg cgcgatcaag gcgcgctgcc ccgacacggc cgtcgaagcg
     1081 ttgacgccgg acttcgccgg caagcttgcc gacgtcgcga cggtcgtcga ttccgaagtc
     1141 gacgtgttcg cgcagaacct ggagacggtc gagcgcttga cgaggaaagt gcgcgacgcg
     1201 cgcgccggct atcgacaaac gctcgacgtg ctcgccttcg cgaagcggca tcgtccgcag
     1261 gtcctgacga agtcgagctt gatgctcggc ctcggcgaga ccgatcaaga gatcgtcgcg
     1321 gcgctcgacg acctgaagcg ggccgccgtc gacatcgtga ccttcggcca ataccttcgc
     1381 ccgacgccga accatctcgc ggtcgagcgc tacgtgactc ccgagcaatt cgagcgctat
     1441 cgagagcagg ggctcgagcg aggtttcctc gaggtcgtcg cgggaccgct cgtccgctcg
     1501 agctaccgtg cagaccgcgt gttcgagaag aacaacgtcg ggctttccgg ctaagtgagc
     1561 gagtttctcg ccgcgtacgg actattcctc gcggagctcg cgaccgtcgc gttggtgctc
     1621 gtcatcgtcg tcgtcctcat cgcctcgtcg cgccggcacg gccataaaga aggcctcgtc
     1681 gtcgagcacc tgaaccggcg gttcgaggac tcggccgaca acttgaagct cgcgatcgaa
     1741 ggcaagggcc gctacaagaa ggcgctgaag gctcggctca aagagcgtaa gcgcgaggac
     1801 aaggcgcgca gcaaggacgg caagccgcgc ctgttcgtcc tcgacttcaa gggcgacttg
     1861 cgcgcgaccg ccgcagcgtc gctgcgcgag gaagtctcgg ccgtgctgcg cgtcgcgcgg
     1921 cccggcgagc aagtgctcgt gcggctcgag aattccggcg gcacggttca cgagcatggc
     1981 ttcgccgctt ctcagctcac gcgcttgaag gagcacggct tgaagctcat cgtcgcggtc
     2041 gacaaggtcg cggcgagcgg cggctatctc atggcctgcg tcgccgatcg gctgatcgcg
     2101 gcgccattcg cgatcgtcgg ctccatcggc gtgctcgcgc agctgccgaa cttccatcgc
     2161 ctgctcgagc aaaagggtgt cgacttcgag caggtcacgg cggggcgcta caagcgcacg
     2221 ctgacgatgt tcggcgagaa cacggacgag ggccgcgcca agctcaagga gcaagtcgag
     2281 gagatccacg cgctgttcaa ggcgcagatc cgcgagcacc ggccgcaggt cgacgtcgag
     2341 agcgtcgcga cgggcgagca ctggcacggc gttcgcgcgc tcgcgctcaa gctcgtcgac
     2401 gagctgaaga cgagcgacga cgtcctgctc gaggcggcga aggatcacga cgtctaccac
     2461 ctcacctaca agcgccgccg aacgctacag gagcgcatcc tgggcggcgc cgagagcctg
     2521 ctctcgcgct gaagagttat gtcgaagcgc ggccctcgcc gtgcggggcg tcgggcatac
     2581 tcgttcgcga cgtccgcgaa cgataacaac gatgaggatg ctcgactccc gctggcaaag
     2641 gcgcctcgcc gcgcttctct cgtgcttgcc ggcttttggc gccggggccg gcgaagaggc
     2701 gggtggtgcg cgcgtgtcgg cctcgagcca gtggtccgcc gccatgagcc gcgcggacgt
     2761 gca
//
LOCUS       DASSNN010000129         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_531627, whole genome shotgun sequence.
ACCESSION   DASSNN010000129 DASSNN010000000
VERSION     DASSNN010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531627"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VFL84_02310"
     CDS             <1..696
                     /locus_tag="VFL84_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607483.1"
                     /translation="RGTAHVNIEGHRGEKGVAPSPSIVIDPPDGKIPYKPEALAQRQK
                     NYEARATADPSLKCYQAGVPRATYVPSPLQILQGPGGTLAIVYQENHAFRVFYPDTRP
                     HFDAADFWMGDTRYRWEGDTLVTSVVALTDQMWIDGAGNFHSPDVHIVERYRMTDADT
                     IEYEARIEDPAVYSKPWTLKIALERVKRRGARIIEDECLEGEDGVRYHISPSDPRNLL
                     KTDYSRWMKPPAE"
     gene            926..1390
                     /locus_tag="VFL84_02315"
     CDS             926..1390
                     /locus_tag="VFL84_02315"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7607484.1"
                     /translation="MCRQCPNCTTLLASALAVAALWPAPGAAHHSFAMYDQSQVKTVT
                     GKLTRFIPGANHAQLIFELLEPDGSPVLDAAGKPALWGVETGPAARIAQQGVTPKNFP
                     VGTIVTVTLNPLRNGKPFGAMPNGTPIVHCGTTMPKGGCTAETGKTYLGQND"
     gene            1404..>2502
                     /locus_tag="VFL84_02320"
     CDS             1404..>2502
                     /locus_tag="VFL84_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607485.1"
                     /translation="MRKSTLIAVAALGFAAGALAQQPQRYSGVDPPPGVEPLPVDLFT
                     SKNYYLDREHWTDPRYTRCNTPAQLTDMWVRQRVGHWGDCSFGMKLEELVSPYPYETA
                     KEHYEALRAQAERKGGVTTHTRETLPDWDGWYFRGTPARPPEGQWLNGNLTQAATMLS
                     LLTPEYQARMTQMNYHEAVNNAPQWMASFCYPEGLMRWWYVWGIRDMEVLVTPKQVQF
                     LAGVADNFIRKVLIGQNHTQLIPQWYGETVGFWDGDTLISWTANVQGWTTSHSMIEFS
                     NSLEVIEITRRADDGEGLVVEAVFYDPEAFVKPLRIVTPWTRTGGIDAPDRRYNFVEC
                     RVENGIVLGPDGRPSQLTPFDEGYVDFLGRPW"
BASE COUNT          481 a          804 c          816 g          401 t
ORIGIN      
        1 cgcggcaccg cgcatgtgaa catcgaaggc caccgcggag agaagggcgt cgcgccatcg
       61 ccgagcatcg tcatcgatcc gccggacggc aagattccgt acaaacccga ggcgctcgcg
      121 caacggcaga aaaattacga agcacgggcg accgcggatc cgtcgctgaa gtgctaccag
      181 gccggagtgc cgcgcgcgac gtacgtgccg agcccgctgc agattctgca ggggccgggc
      241 ggcacgctcg caatcgtgta ccaggagaac cacgcgttcc gcgtcttcta ccccgatacg
      301 cggccgcact tcgacgcggc cgatttctgg atgggcgaca cgcgctaccg ctgggaaggc
      361 gatacgctcg tcacgagcgt cgtggctctg accgaccaga tgtggatcga cggggccggc
      421 aactttcaca gccccgacgt gcacatcgtc gagcgctacc gcatgaccga tgccgacacg
      481 atcgagtacg aggcgcgcat cgaggatccg gccgtgtatt cgaagccgtg gacgctgaaa
      541 atcgcgctcg agcgcgtcaa gcggcgcggc gcgcgcatca tcgaggacga gtgcctcgag
      601 ggcgaggacg gcgtgcgcta tcacatctcg ccgtccgacc cgcgcaatct cttgaagacc
      661 gactactcgc gctggatgaa accgccagcg gaatagcggc aatcagcgct ccgaaatcgt
      721 gcgcgccgat tgttgctatt cgtatttccg atttgaggtc tgtggtgccg gtgttacggt
      781 tcggttcagc cgaagctccg gcacttcggt tgaaccgaac cgtaacaccg gcaccacact
      841 cgctgctaag ttgacgactg atgaccgagt cgccgacgct tcgctgcggg cggcgaattc
      901 gcggaaatca taaggagagt caaggatgtg cagacagtgc ccgaattgca cgacgcttct
      961 cgcttcggcg ctcgcggtcg cggcgctgtg gcccgcgccc ggcgccgctc accattcctt
     1021 cgcgatgtac gaccagtccc aggtcaagac ggtgaccggg aagctgaccc ggttcatccc
     1081 cggcgcgaac cacgcacagc tcatcttcga gctgctcgag cccgatggat cgccggtcct
     1141 cgatgcggcc ggtaagccgg cgctgtgggg cgtcgaaaca ggccccgcgg cgcgtatcgc
     1201 gcaacagggc gtgacgccga agaacttccc cgtaggtacg atcgtgaccg tgacgctgaa
     1261 cccgctgcgc aacggcaagc cgttcggcgc aatgccgaat ggaacgccga tcgtgcattg
     1321 cggaacgacg atgccgaagg gcggttgcac ggccgagacc ggcaagactt atctcggtca
     1381 gaacgactag aggacggccg aacatgcgaa agtcgacttt gatcgcggtg gccgcgctcg
     1441 gatttgccgc gggcgcgcta gcgcagcagc cgcaacggta tagcggcgtc gacccgccgc
     1501 ccggcgtcga gccgctgccc gtcgatctct tcacgagcaa gaattactac ttggaccgcg
     1561 agcactggac ggacccgcgt tacacgcgct gcaacacgcc ggcccagctc acggacatgt
     1621 gggtgcggca gcgcgtcggg cattggggcg actgcagctt cggcatgaag ctcgaagagc
     1681 tcgtcagccc gtacccgtac gagacggcca aagagcacta cgaagcgttg cgagcgcaag
     1741 cggagcgcaa gggcggcgtg accacgcata cgcgcgagac gctccccgac tgggacggct
     1801 ggtatttccg cggcactcct gcgcggccgc cggaaggtca atggctgaac ggcaacttga
     1861 cgcaagccgc gacgatgttg tcgctgctga cgcccgagta tcaggcgcgc atgacgcaga
     1921 tgaactacca cgaggccgtc aataacgcgc cgcagtggat ggcctcgttc tgctaccccg
     1981 aagggctcat gcgctggtgg tacgtctggg gcatcaggga catggaagtg ctcgtgacgc
     2041 cgaagcaggt gcagttcctc gccggcgttg ccgacaactt catccgcaaa gtgctgatcg
     2101 gccagaatca cacgcagctg atcccacagt ggtacggcga gacggtcggg ttctgggacg
     2161 gcgatacctt gatctcgtgg acggccaacg tgcagggctg gacgacgagc cattcgatga
     2221 tcgagttcag caactcgctc gaggtcatcg agatcacgcg ccgcgccgac gacggcgagg
     2281 ggctcgtcgt cgaagccgtc ttttacgacc ccgaagcctt cgtcaagccg ttgcgcatcg
     2341 tgacgccctg gacacgcacg ggcggcatcg atgctcccga ccggcggtac aacttcgtcg
     2401 aatgccgtgt cgagaacggc atcgtgctcg ggcctgacgg ccggccctcg cagctcacgc
     2461 cgttcgacga gggctatgtc gatttcctcg gccggccgtg gg
//
LOCUS       DASSNN010000130         4997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_265965, whole genome shotgun sequence.
ACCESSION   DASSNN010000130 DASSNN010000000
VERSION     DASSNN010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265965"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFL84_02325"
     CDS             <1..189
                     /locus_tag="VFL84_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607486.1"
                     /translation="TESWSQSLQDGERTLREVKELERLLQAGQVGEATLELQRLRQRT
                     LSNLAAFMSNPHAAGVRV"
     gene            366..821
                     /locus_tag="VFL84_02330"
     CDS             366..821
                     /locus_tag="VFL84_02330"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7607487.1"
                     /translation="MKTPSVRDATSTDTDAVVSILREFHEERAIASVRAARDAVQACI
                     DHDDRDLIVAEIDGVVVGYTAVHWVPFPMLQGREAYVSDLLIRREWRDRGLGRLLLAE
                     VEERARARRCPRVMLNNRRAAESFKRGFFSKAGFREREDFANFVKTLHE"
     gene            814..1566
                     /locus_tag="VFL84_02335"
     CDS             814..1566
                     /locus_tag="VFL84_02335"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HET7607488.1"
                     /translation="MSKRAAMLSVNLNKVALLRNARRGATPSVLEAAGICLAEGAGGV
                     TVHPRPDGRHIRASDVSELSELVKSTRTEFNVEGNPEPSFLELVHGTRPTQCTLVPDA
                     PDALTSDHGWDFDAHGKQLRPIIEQLRTAGIRVSLFVEPEPDDVERARALGADRVELY
                     TESYARAHGTEGEARELRRFAIAAEKADEVGLGVNAGHDLNLQNLSAFCAAVPHVLEV
                     SIGHALVSYSLEVGLRRAVRDYVHALAAEPST"
     gene            1563..1853
                     /locus_tag="VFL84_02340"
     CDS             1563..1853
                     /locus_tag="VFL84_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607489.1"
                     /translation="MKRALAGLAAIVALAWMTFHAFVIVLLVRSEVVPALAQGTFNIQ
                     TSSMILNRLWEGNELYWLIAAHAALVLILAVVAMRTARFAVRGTRRDSPVPT"
     gene            complement(1872..3914)
                     /locus_tag="VFL84_02345"
     CDS             complement(1872..3914)
                     /locus_tag="VFL84_02345"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HET7607490.1"
                     /translation="MARNKLSSLALALIGLWSASAAAEVVRIEVDTRADVAAGAAGAA
                     YGVAGAYEKLRGTLHFAVDPLASANGNITDLDLARPNAAGLVEFSADFYLLKPKDVAR
                     GNGALLFEVSNRGGKGILPMLSRAAASRDPTEARELGDGFLLEQGFSLLWVGWQLDPP
                     PTQANLLRVYPPTTDPGLSVTGLVRSDFVVRSAGVRDHSLADGEHVPYPVADPTSEAN
                     TLTVRDTPLGERRTIPRSEWQFARVDGSGNVVADPTRVYMTSGFEPKKIYEVVYTSRN
                     PPLAGLGLAAIRDAVSRLKNEGAQELGIGEDSYDRALGFGISQSGRVLRTFLYEGFNA
                     DERNRRVFDGVLAHIAGGARGGFNTRFAQASRSSSSYLYPNELFPFTDAVQADSTSGI
                     SNGLLGRVDPKHVPRIFYTNSANEYWRGSAALTHVTPDGRRDFPPPGTTRIYFFAGTQ
                     HGPAPFPPRVNEGRLAGNPNAYSWFMRSLLLKLDAWVAHGVAPPPSVYPTLADGTLVP
                     RSQLKFPKIPGVDVPRAPSGPLRLELGPSFASSGIATIEPPRVGEPFPVLLPQVDADG
                     NDVAGLRSPELAVPLATYMGWQLYKPELGRPDELVSLQGSFAPFPLDTAERVRTGDPR
                     RAILERYPDRNGYLALVEQVAKLLIADGYLRGEDLPQIVAQAGDRWRVLVDERAAR"
     gene            4036..>4997
                     /gene="glyA"
                     /locus_tag="VFL84_02350"
     CDS             4036..>4997
                     /gene="glyA"
                     /locus_tag="VFL84_02350"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET7607491.1"
                     /translation="MSDMPALRATDPEIHRLIALETKREADYIRLIPSENYASAAVME
                     ATGSVLANKYSEGYPGKRYYEGQEYIDQVETLAIERAKSLFGMQHANVQPYSGSPANQ
                     AVYLALAKPGEHVMGLGLPFGGHLTHGWGVNFSGIVYRADQYDVDPETHRVDLNRVET
                     LARETKPKLIFCGGTAYPRTWDYAGFAQIAKAVGAVLVADIAHIAGLIVAGAHPHPYP
                     HATIVTTTTHKTLRGPRGGMILCDASHAKDIDRAVFPGLQGGPHNHTTAAIAVALKEA
                     STAEFRDYGQQIVKNAKALHAAGIECNYNTSPFDPRKPFDPSGLR"
BASE COUNT          858 a         1621 c         1693 g          825 t
ORIGIN      
        1 acggagtcgt ggagccaaag ccttcaggac ggcgagcgga cgctccgcga agtgaaggag
       61 ctggagcgac tgctgcaagc cggtcaagtc ggcgaggcaa cgctcgagtt gcagcgcctt
      121 cggcagcgca cactttcgaa cctggctgcc ttcatgagca accctcatgc cgcgggtgtc
      181 cgtgtttgag ctctgagggt cttttgcagc gagatcccgg tggagctgcc gctccccaag
      241 aaacaacggc gaggaatctg gccagcgcag aattctcgct acacaaccgc tctgcaatcg
      301 cgagcctggt tgagatcgtt gtctctaatg tgccgcgtta cctgacacag attcggtagg
      361 ccctcatgaa aacgccctcc gtgcgcgacg cgacgagcac cgataccgac gccgtcgtca
      421 gcatattgcg ggagtttcac gaagagcggg cgatagcctc cgtgcgcgcg gcccgagatg
      481 ctgttcaagc gtgcatcgat cacgatgacc gggatctaat cgtcgcagag atcgacggtg
      541 tggttgtcgg ctacaccgca gtgcactggg tcccttttcc tatgttgcaa ggtcgggaag
      601 cgtacgtctc cgatctgcta attcgacgcg agtggcgtga tcggggcctt gggcgtttgt
      661 tgcttgcgga ggtcgaggag cgtgctcgtg ctcggcgctg tccgagggtc atgctaaaca
      721 atcgccgggc tgcagaatcg ttcaagagag gcttcttctc caaggcaggg tttcgcgagc
      781 gcgaagactt cgccaacttc gtcaagacac tgcatgagta agcgcgcggc catgctgagt
      841 gtgaacctca acaaagtcgc attgctgcgc aatgcgcggc gcggcgccac gccgagcgtg
      901 ctggaagcgg ccggcatttg cctcgcggaa ggagccggcg gagttaccgt tcatccgcgc
      961 ccggatggcc gccacatacg agcgtcggac gttagcgagc tctccgagct cgtcaaatca
     1021 acgcgaactg aattcaacgt cgagggcaat ccagaaccgt ccttcctcga gctggtccac
     1081 ggcacaagac cgacgcagtg cacgctcgtg ccggacgcgc ccgacgcgct gacgtcggat
     1141 cacggctggg acttcgatgc acacggaaag cagcttcgcc cgatcatcga gcaattgcgc
     1201 acggccggta ttcgcgtgag cctctttgtc gagcccgagc cggacgacgt cgagcgcgca
     1261 cgagcgcttg gggccgacag agtcgagctg tacacggaat cctatgcgcg cgctcacgga
     1321 accgaaggcg aggcacgaga gttgcgacgt ttcgcgatcg ccgccgagaa ggcggacgaa
     1381 gtcgggttag gcgtcaacgc aggccacgat ctcaatctgc agaacctttc cgccttttgc
     1441 gcggcggtac cgcatgtcct cgaggtttcg atcggccatg cgctggtctc ctattccctc
     1501 gaggtcgggt tacggcgcgc cgtgcgcgat tacgttcatg cacttgcggc ggagccgtcg
     1561 acgtgaaaag agcgctagcc ggtcttgcgg cgattgttgc gcttgcatgg atgacgttcc
     1621 atgccttcgt catcgtgttg ctcgtgcgga gcgaagtggt tccggcgctc gcgcaaggga
     1681 ccttcaacat tcaaacgagc tcgatgattc tgaaccgact ctgggaaggc aacgagctgt
     1741 attggctcat agccgcacat gcggcgctcg tgttgattct cgcggtcgta gctatgcgga
     1801 cggccagatt cgcggttcga ggcactcgcc gggatagccc ggtcccgacg tagaccttgc
     1861 gcagctccct gctagcgcgc ggcgcgttcg tcgacgagca cgcgccagcg atcgccggcc
     1921 tgcgcaacga tttgcggaag atcctcgccg cgcagatacc cgtccgcgat cagcagcttc
     1981 gcgacctgct cgacgagcgc gagatagccg ttgcgatccg ggtaacgctc gagaatcgcg
     2041 cggcgcggat cgccggttcg cacgcgttcg gcggtgtcga gcgggaacgg cgcgaacgag
     2101 ccttgcaagc tgacgagctc gtcggggcgg ccaagctcgg gcttgtagag ctgccagccc
     2161 atgtaggtcg cgagcggcac ggcgagctcc ggcgaccgca gtcccgcgac gtcgttgccg
     2221 tcggcgtcga cttgcggcag cagcacgggg aacggctcgc cgacgcgcgg cggctcgatc
     2281 gtcgcgatgc cgctgctcgc gaagctcggc ccgagctcga ggcgcagcgg gccgctcggc
     2341 gcgcgcggca cgtcgacgcc cgggatcttg gggaacttca gctgcgagcg cggcacgagc
     2401 gtcccgtccg cgagcgtcgg atagacgctc gggggcgggg ccacgccgtg cgcgacccac
     2461 gcatcgagct tcagcagcag cgagcgcatg aaccacgagt acgcgttcgg attgccggcg
     2521 agacggcctt cgttgacgcg cggcggaaac ggcgcaggcc cgtgctgcgt gcccgcgaag
     2581 aagtaaatac gcgtggtgcc gggcggcggg aaatcgcgcc ggccgtcggg cgtcacgtgg
     2641 gtcagcgcag ccgagccgcg ccagtattcg ttcgccgagt tcgtgtagaa gatgcgcggc
     2701 acgtgcttcg ggtccacgcg cccgagcaga ccgttgctga tcccgctcgt cgagtcggcc
     2761 tgcaccgcgt cggtgaacgg aaagagctcg ttcggataca ggtagctcga gctcgaccgc
     2821 gacgcttgcg cgaagcgcgt gttgaagccg ccgcgagcac cgccggcgat gtgcgcgagc
     2881 acgccgtcga acacgcgtcg gttgcgctcg tccgcgttga agccttcgta caggaacgtg
     2941 cgcagcacgc ggccgctttg cgagatgccg aagccgagcg cgcggtcgta cgagtcttcg
     3001 ccgatcccga gctcttgcgc gccttcgttc ttgagccggc tcacggcgtc gcggatcgcg
     3061 gccaatccga ggcccgcgag cggcggattc ctggacgtgt agacgacctc gtagatcttc
     3121 ttcggctcga atccgcttgt catgtagacg cgggtcgggt ccgcaacgac gttgccgctg
     3181 ccgtcgacgc gcgcgaactg ccactcgctg cgcggaatcg tgcggcgctc gccgagcggc
     3241 gtgtcgcgca cggtcagcgt gttcgcttcg ctcgtgggat ccgcgacggg gtagggcaca
     3301 tgctcgccgt cggcgagcga gtggtcgcga acgcccgcgg aacgcacgac gaaatcgctg
     3361 cggacgaggc ccgtgacgct caaccccggg tccgtggtcg gcggatagac gcgcaacagg
     3421 ttcgcttgcg tcggcggcgg gtcgagctgc cagccgaccc agagcagcga gaacccctgc
     3481 tcgagcaaga agccgtcgcc gagctcgcgg gcctcggtcg ggtcgcggct cgcggccgcg
     3541 cggctcagca tcggcaggat gcctttaccg ccgcggttcg atacctcgaa cagcagcgcg
     3601 ccgttgccgc gcgcgacgtc cttcggcttg agcaagtaga agtcggcgct gaactcgacg
     3661 aggcccgccg cattggggcg cgcgaggtcg agatccgtga tgttgccgtt cgccgaagct
     3721 aaagggtcta cggcgaagtg cagcgtgccg cgcagcttct cgtaggcgcc ggccacaccg
     3781 taagcggcgc cggcggcgcc ggcggcgacg tcggcgcgcg tgtcgacctc gatgcggacg
     3841 acctcggcgg cagccgacgc gctccacaac ccgatgagcg cgagcgcgag gctcgagagc
     3901 ttgttacgtg ccacgccgac cccctggtgc ggaacttgct tgccgtcgct gctaggcgag
     3961 cgcggcgtcc tgtagactat cgccccccaa ccgcggcctt ccggccgatc ttaagccctg
     4021 tttcgaggaa tgcgaatgtc cgacatgcct gcgctgcgcg cgaccgaccc cgagatccac
     4081 cgcctgatcg cgctcgagac gaagcgcgaa gccgactaca tccggctgat cccgtccgag
     4141 aactacgcct cggccgcggt catggaagcg acgggctccg tgctcgcgaa caagtactcc
     4201 gaaggctatc ccggcaagcg ctattacgaa gggcaggagt acatcgatca ggtcgagacg
     4261 ctcgcgatcg agcgcgcgaa gagcctgttc ggcatgcagc acgcgaacgt gcagccgtat
     4321 tcgggctcgc cggcgaatca ggccgtctat ctcgcgctcg cgaagccggg cgagcatgtc
     4381 atgggcctcg ggttgccgtt cggcggccac ttgacgcacg gctggggcgt gaacttctcc
     4441 ggcatcgtct atcgcgcgga ccagtacgac gtcgatcccg agacgcaccg cgtcgatctg
     4501 aaccgcgtcg agacgctcgc gagagagacg aaaccgaagc tgatcttctg cggcggcacg
     4561 gcctatccgc gcacgtggga ttacgcgggc ttcgcgcaga tcgcgaaggc cgtcggcgcc
     4621 gtgctcgtcg ccgacatcgc gcacatcgcg ggcttgatcg tcgccggcgc gcatccgcat
     4681 ccgtatccgc acgcgacgat cgtcacgacg acgacgcaca agacgctgcg cggcccgcgc
     4741 ggcggcatga tcttgtgcga cgcgagccac gcgaaggaca tcgatcgcgc cgtgttcccg
     4801 ggcctgcaag gcggcccgca caaccacacg acggccgcca tcgcggtcgc gctgaaggaa
     4861 gcgtcgacgg cggagttccg cgactacggc cagcagatcg tcaagaacgc gaaggcgctg
     4921 cacgcggccg gcatcgagtg caactacaac acgagtccgt tcgacccgcg caagccgttc
     4981 gacccgagcg ggcttcg
//
LOCUS       DASSNN010000131         3502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1540231, whole genome shotgun sequence.
ACCESSION   DASSNN010000131 DASSNN010000000
VERSION     DASSNN010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540231"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3502
                     /locus_tag="VFL84_02355"
     CDS             <1..>3502
                     /locus_tag="VFL84_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7607492.1"
                     /translation="LSIAIAAALGTVGPVVFSSGAFAQEPGQAAAPPDDLEEVVVTGS
                     RIQRRDYTSNSPIVTVEQDAFEAQVGLNFEAYLNQLPNYNPAAAPTTTQADVQITPVN
                     SVGIASISLRGFGPNRNLVLVDGKRLVPINALMVTDINAIPSALIERVETITGGASAV
                     YGADAVGGVTNFILRDDFEGIDFDVQYGTTDQGGGEERRISSVLGANLAGGRGNVTLG
                     VERYDRDEVLERDREWYRDRYADPHAPGTFTFLQGTTHYNCLTNCPSPGAVEAVFGAP
                     ATSVFNPLSFNVFRQFVFNADETLFVENSAAGLANFTGGPHFTFFPSEIFDASVPGNT
                     SVIEGLKWHNLKALVSSPQERYSFFASGNYDFTDRIRLTSRATLAESETRTVLFGTNA
                     IFGWEGTVPYVPATDSPILPTLNYNDPAVVAAAVADPTNPAFRNPAFVPTGTPGAGWP
                     VPVELAALLNSRPAGTYCISGTPGCGAPGSGTYATTNPALAGLATTGDWMPGWSPDYS
                     LPPRSTINTNTVWQLEAGLDIDLAENWTSEIYVSHGQSSAYNNAAGNLSLTRYRELIR
                     RPDYGRNAAISGNATGASPGFGAADVTCTSGFHDTFFRGDEPLSQDCFNAVNATLQTR
                     AQNIQDILEVNFEGQLAELRAGELRLAAGYQRRENSATFVPDILQSTVSFTDQVVGVY
                     PTGYMDASTSVDDLYAEMLVPLIAGERGQRFELELGFRASDYEHTERENTYKTLVNWQ
                     INAWVRFRGGYNRATRAPNLGELFLAPQEIFQIGGNNFGDPCGLRSTAPYGAGGTGPD
                     PQFNPATETQPQLASGQTPQGAASARLICEALMGPAAANQFYNVANAAQGGGSPFNWV
                     VQQGNPDLRSETADTWTWGIVMSPPFERPWLSGINLSLDYYRVEIEDAIMLYSVDFAN
                     FSCFGSTLVTSPADAAAQAASPECQRVPRNQALGGPLTTSLSYDNQATIDTAGLDLGL
                     NWRLQLSDAGRDLPGSLMFNLQATWLDYYRTKQSPAAFDVETDWKGSLGPNLSGTNPG
                     AYDYRLFGSVGYFANAWGVNLRWRYLPDVRTAAFASQQAIKENNARVAAGGAGVILSY
                     TPTTELETDNYSVFDLSFNWSVGDRLQFRGGVTNLLDTEPEEVGSTAGYPIGTDLAAV
                     CGGAPGCVRPLAFSLPSTGG"
BASE COUNT          618 a         1156 c         1155 g          573 t
ORIGIN      
        1 cgctttcgat tgcgatcgca gcggcgctcg gcaccgtggg gcccgtcgtc ttttcgtccg
       61 gtgcgtttgc acaagagccc ggtcaggcgg cggcgccgcc cgacgatctc gaagaagtcg
      121 tcgttaccgg ctcgcgcatt caacgacgcg actacacgtc gaacagtccg atcgtgacgg
      181 tcgagcaaga cgcgttcgag gcgcaggtgg gactgaactt cgaggcctac ctgaatcagc
      241 tgccgaacta caaccccgcc gcggcgccga cgacgacgca ggccgacgtg cagatcacgc
      301 cggtgaactc ggtcggcatc gcgtcgatct cgctgcgcgg attcggcccg aaccgcaatc
      361 tcgtgctcgt cgacggcaaa cggctcgttc cgatcaacgc gctaatggtg accgacatca
      421 acgcgatacc gtcggcattg atcgagcgcg tggagaccat cacgggcggc gcgtccgcgg
      481 tgtacggggc cgacgccgtc ggcggcgtca cgaacttcat cctgcgcgac gacttcgaag
      541 gcatcgattt cgacgttcag tacggcacca ccgatcaagg cggcggcgag gaacgccgga
      601 tctcctcggt gctcggcgca aatctcgcgg gcggccgcgg caatgtcacg ctgggcgtcg
      661 agcggtacga ccgcgacgag gtgctcgaga gagatcgaga gtggtacagg gaccgctacg
      721 cggatccgca tgcgcccggc accttcacgt tcttgcaggg cacgacgcac tacaactgtc
      781 tgaccaattg cccgagcccg ggcgccgtgg aagcagtatt cggagcgccc gcgacgagcg
      841 tgttcaatcc gctgtcgttc aacgtgttca ggcagttcgt tttcaacgcg gacgagacgc
      901 tgttcgtcga gaacagcgcg gcaggcctgg ccaacttcac gggcgggccg cacttcacgt
      961 tctttccgtc ggagatcttc gatgcctccg tgcccggcaa cacgagcgtg atcgaggggc
     1021 tcaagtggca caacctcaaa gcgttggtca gctcgccgca ggagcgctat tcgttcttcg
     1081 cttcgggcaa ctacgacttc accgacagga tccgcttgac ctcgcgagcg accctcgcgg
     1141 agagcgagac gcgcacggtc ttgttcggca cgaacgcgat cttcggctgg gaaggcactg
     1201 ttccctatgt cccggcgacc gacagcccga tcttgcccac gctgaactac aacgatcccg
     1261 ccgtcgtggc cgccgccgtc gcggacccga cgaacccggc ctttaggaat ccggcgttcg
     1321 ttccgaccgg caccccgggc gcgggctggc ccgtccccgt cgagctcgcc gcgctgctca
     1381 acagccgccc ggcgggcacg tactgcatca gcggcacgcc gggctgcggc gcgccggggt
     1441 ccggaacgta cgcgacgacg aatccggcgc tggccgggct cgcgacgacg ggcgactgga
     1501 tgccgggctg gagcccggac tatagcctgc ctcctcgcag cacgatcaac acgaacacgg
     1561 tctggcagct cgaggccggg ctcgacatcg atctcgcgga gaactggacg agcgagatct
     1621 acgtgtcgca cggccaatcc tcggcctaca acaacgcggc gggcaacttg tcgctcacgc
     1681 gctatcgaga gctgatccgg cggccggact atgggcgcaa cgcggcgatt tcgggcaacg
     1741 cgacgggcgc gtcgccgggc ttcggcgcgg ccgacgtcac gtgcacgagc ggtttccacg
     1801 atacgttctt caggggcgac gagccgttgt cgcaagactg cttcaacgcg gtcaacgcga
     1861 cgctgcaaac gcgcgcgcag aacatccagg acatcctcga ggtgaacttc gaggggcagc
     1921 tggccgagct gcgcgccggc gagctgcggc tcgcggcggg ctatcagcgg cgagaaaact
     1981 cggcgacgtt cgtgcccgac atcttgcagt cgacggtgtc gttcacggat caggtggtcg
     2041 gcgtgtatcc aacgggctac atggatgcct cgacatcggt cgacgatctc tacgcggaga
     2101 tgctcgtgcc gctgatagcc ggagaacgcg ggcagcggtt cgagctcgag ctcggcttcc
     2161 gagcgtccga ctacgagcac acggagcgcg agaacacgta caagacactg gtcaattggc
     2221 agatcaacgc ttgggtacgc ttccgcggcg gctacaaccg cgccacgcgc gcgccgaatc
     2281 tcggcgagct attcttggct ccgcaagaga ttttccagat cggcggcaac aacttcggcg
     2341 acccgtgcgg attgcgctcg acggcgccgt acggcgccgg cggcacgggc cccgacccac
     2401 agttcaatcc cgcgaccgaa acgcagccgc agctcgcgtc gggacagacg ccgcaaggcg
     2461 ccgccagcgc gcgcttgatt tgcgaagcgt tgatgggccc ggcggcggcg aatcagttct
     2521 acaacgtcgc gaatgccgct caaggcggag gctcgccgtt caactgggtc gtgcagcaag
     2581 gcaacccgga cttgagatcg gagactgccg atacgtggac atggggaatc gtgatgtcgc
     2641 cgccgttcga gcgtccttgg ctcagcggaa tcaacctgtc gctcgattac taccgcgtcg
     2701 agatcgagga cgcgatcatg ctgtactcgg tcgacttcgc gaacttcagc tgcttcggct
     2761 cgacgctcgt gacgagcccc gccgatgcgg cggcgcaagc cgcgtcgccg gagtgccagc
     2821 gcgtgccgcg caatcaggcg ctcggcggcc cgctgacgac gtcgctgtcg tatgacaacc
     2881 aggcgacgat cgacactgcg gggctcgacc tcggactgaa ctggaggttg cagctgtcgg
     2941 atgccggccg cgatcttccc ggcagcttga tgttcaactt gcaagcgacg tggctcgact
     3001 actaccgcac gaagcagtcg ccggcggcgt tcgacgtcga aacggattgg aagggatcgt
     3061 tgggcccgaa cctgtcgggc acgaaccccg gcgcgtacga ttatcgcttg ttcggctcgg
     3121 tcgggtactt cgcgaatgct tggggcgtca acttgcggtg gcgctacttg ccggacgtgc
     3181 gcacggcggc gttcgcgagc cagcaagcca tcaaggaaaa caacgcgcgc gtcgccgccg
     3241 gcggcgcggg cgtcatcttg agctatacgc cgaccaccga gctcgaaacc gacaactaca
     3301 gcgtgttcga cctctcgttc aactggagcg tcggcgaccg gctgcagttt cgcggcggag
     3361 tcacgaacct gctcgacacc gagcccgagg aggtcggcag cactgccggc tacccgatcg
     3421 gcacggacct cgccgccgtc tgcggcggtg cgccgggttg cgttcgcccg ctggcgttca
     3481 gtctgccgag cacgggaggc tt
//
LOCUS       DASSNN010000132         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_27771, whole genome shotgun sequence.
ACCESSION   DASSNN010000132 DASSNN010000000
VERSION     DASSNN010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27771"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFL84_02360"
     CDS             complement(<1..450)
                     /locus_tag="VFL84_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7607493.1"
                     /translation="MLSIERPRDNRWPAALFSPRDAADDLNGVLSSALRLRPQPTFLA
                     VNIWAGSEGTIVRVFIPGVSPDRLNLTVERQTLTLAGERDPETIEGESMQLRAERMHG
                     RFSRTLLLPFRVDPDQAQARFGLGMLTIELPRPEADRPRRIKIAATAD"
     gene            657..1484
                     /locus_tag="VFL84_02365"
     CDS             657..1484
                     /locus_tag="VFL84_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET7607494.1"
                     /translation="MDVFWLIFLFSMLAPVVRQRLLEAARLRRIARLERQRRSRVIVL
                     VHRQETMRLLGVPLLRYLDINDSEEVLRAIQLTDDDLPLDIVLHTPGGLVLAAAQIAT
                     ALRAYKSKVTVFVPHHAMSGGTLIALAAGEIVMSRHAVLGPLDPQLKGMPAASLISVV
                     TSKPIAEVDDETLVLADVGQKAIAQVRHAVRHLLDGRIPAAQADAVAEQLSSGKWTHD
                     YPISAAEAKGLGLPVSTNMPNEVLELMALYPQPVRAQGSGGVEYLPWPRRKRTGPSN"
     gene            1554..1991
                     /locus_tag="VFL84_02370"
     CDS             1554..1991
                     /locus_tag="VFL84_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HET7607495.1"
                     /translation="MAADAGRPHATLYGRFEHGVVLILTGVIAVVIVATLWSLILTVV
                     QGIVVPAGSFDPTEHMVFQASFGMIFTVLIALEFKRSLLVLAERRESIVQVRTVILIA
                     LLAIVRKLIIFDFAVSDWRELLALGGVHWLVRDQDRRRSSSRA"
BASE COUNT          368 a          673 c          700 g          398 t
ORIGIN      
        1 gtccgccgtt gcggcgatct tgatgcggcg cggtcgatcc gcctcggggc ggggaagctc
       61 gatcgtcagc atgccgaggc cgaaccgcgc ttgcgcctgg tcgggatcca ctcgaaacgg
      121 caacagaagg gtgcgtgaga agcgtccatg catccgctcg gcgcgcagct gcatcgactc
      181 tccttcgatg gtctccgggt cgcgctctcc ggcgagcgtc aaggtttgtc gctcgacggt
      241 caggttgagg cggtccgggc tcacgccggg tatgaacacc cgcacgatcg tgccttcgct
      301 cccggcccag atgttcaccg cgagaaaggt cggctgcgga cgtagacgca gcgccgacga
      361 aagcacgccg ttcagatcgt cggccgcatc ccgcgggctg aagagcgccg caggccaacg
      421 gttgtcgcgg ggcctttcga tggaaagcat gtccgactcc tcacgcacca aactcatttg
      481 cactgacgtg tagtcaagac gatcgctctg ctcctcgcat tgacggtcgt caattcaacg
      541 gtgccgtggg tatggtcatg tcggaaaggc gcgcgccgcg cgatggcgcc tgcgagcgcg
      601 tgcggcccga gcaaggaggc gcggcggtgg gtgagcaaat cggagcggcg gttgtcatgg
      661 acgtgttttg gctgattttc ctcttttcga tgctggcgcc cgtggttcgg cagcgcctgc
      721 tcgaggccgc gcgcttgcgg cgtatcgcgc ggctggagcg gcagcgacgg tcgcgcgtca
      781 tcgtgctagt gcaccgccaa gaaacgatgc gcctgctcgg tgttccgctt ctgcgctatc
      841 tggacatcaa cgattccgag gaggttctgc gtgcgatcca gctgaccgat gacgacttgc
      901 ccctcgacat cgtgttgcac actccgggcg ggttggtgct cgcggcggcg cagatcgcga
      961 ccgccctgcg cgcctacaaa tcgaaagtga cggtcttcgt tccccatcac gcgatgtctg
     1021 gaggtacctt gatcgcgtta gctgccggcg agatcgtgat gagccggcac gccgtgctcg
     1081 gccccctcga tccccagctg aagggcatgc ccgccgcgtc gttgatcagc gtcgtgacca
     1141 gcaaacctat cgccgaggtc gacgacgaaa cgctcgtgct ggcggacgtt ggacagaagg
     1201 cgatcgcgca agtccggcac gcagtgcggc acttgctcga cggacggatc ccggctgctc
     1261 aagcggatgc cgtcgcagaa cagctgtcat cgggcaaatg gactcacgac tatccgatct
     1321 cggcggccga agcgaaaggc ctcggcctgc cggtgagcac gaacatgccg aacgaagtgc
     1381 tggagctgat ggccctttat ccgcagccgg tgcgggcgca aggcagcggc ggcgtcgagt
     1441 acctgccctg gccacggcga aagaggaccg gcccaagcaa ttgagccggc ggcggctatt
     1501 ggctcggcat gacacgcgag gttcagctga tgcgcgaaat agacgatcga cccgtggcag
     1561 cggacgccgg tcgccctcac gcgacgctgt acggaaggtt cgagcacggc gtcgtcctca
     1621 ttctgaccgg cgtgatcgcc gtggtgatcg tcgccacgct atggagcctg atcctcacgg
     1681 ttgtccaggg tatcgtcgtg cctgcgggca gcttcgatcc caccgagcac atggtttttc
     1741 aggccagctt cggcatgatt ttcaccgtgt tgatcgcgct cgagttcaag cgctccttgt
     1801 tggtgctcgc cgagcgcaga gagagcatcg tgcaagttcg cacggtcatt ctgattgcac
     1861 tgctcgcgat cgtacgcaag ctgatcatct tcgacttcgc cgtaagcgat tggcgcgagt
     1921 tgctcgcgct cggcggcgtg cattggttgg tgcgggacca ggatcgacgc aggtcgtcga
     1981 gccgagcatg aacgccaaca cgttgacggg cgtcaatgcc cggcgcgtcc ccatcgctca
     2041 acctgcactt catggcacgc accgacacaa ccgagcagac aaaccttctg cgcggcgcta
     2101 aggcaccggg cttcaagctc cactcgactc cggatcaat
//
LOCUS       DASSNN010000133         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1778496, whole genome shotgun sequence.
ACCESSION   DASSNN010000133 DASSNN010000000
VERSION     DASSNN010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778496"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1365)
                     /locus_tag="VFL84_02375"
     CDS             complement(94..1365)
                     /locus_tag="VFL84_02375"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7607496.1"
                     /translation="MNVTPTRLAAACAALLAAGAASAHHGFGNFDRSREVALEGTITS
                     IDFVNPHAYVYFEATNPDGTKVAKRCEMRAATVLRRSGWSAEMFKTGETIKIQGAPDR
                     FDVNSCYVHTVVFADGTTADRYAQLSKPTQAAPSAAPRAARTASGEPNITGDWAPEQV
                     VMTDPRGRSGALVPVSRVDDFEPGATAPPAGAGGNRGRPRVTYTEAGKAAADAFRGGT
                     TDNPRMRCETTSILFDWTFDGPVNRITQNGDTITLQYGQLGFTRTIHMNAEHPANIEP
                     SRAGHSIGRWENDVLVVDTIGFTEGYLNPPIKNSEQLHVVERFSLDPNAMTVTREYTA
                     TDPVYYTDQYSGSDTIGVADLPYSPDKCTELTFVDFSADGVAPDASSGGIGPQGAAAA
                     PAQTAAAATDAAPAAEETKEAACWEVWKWFD"
     gene            1528..2046
                     /locus_tag="VFL84_02380"
     CDS             1528..2046
                     /locus_tag="VFL84_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7607497.1"
                     /translation="MPPAVFVDRTLGFLVADIARLLRRSMDRRLQSLGLTQAQWRAIV
                     HLSRSEGMTQAALAESLEIAPITLTRLIDRMESAGWVERRTHPLDRRAVQLYLTAQSQ
                     PILEQMHARAADTLNEAMRGISPRAQRQLVASLEQLKHNLIAAETAAVSPEPSGIRHG
                     RPRLAKSARARV"
     gene            2114..>2830
                     /locus_tag="VFL84_02385"
     CDS             2114..>2830
                     /locus_tag="VFL84_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET7607498.1"
                     /translation="MGPIVALAVGGYFYYTSGRIVGTDNAYVKSDVGVVSAEVAGPIA
                     ALNVHENDRVEAGEVLFTIDDRPFRVALDRGNAQLAAIKDFVESTKASYRQSLEQLAL
                     ARTNAAYEQRELDRLAALAERKLASDVDVDEQRHDRDVANQEIQVTERALDAIRARLG
                     GDLDRPVTEQAAYLAAKSMRDAAELDLDRTVVRAPFSGIASQVPTVGHHVQPGAPVMT
                     IVADRDMWIEANFKETDLTHV"
BASE COUNT          436 a          962 c          940 g          492 t
ORIGIN      
        1 caaggctcaa ggtcgcaagc atgatcagtt gtcgtcgcat gactttcctc gatgtcattt
       61 gctgcaggct acttcttgtc gccctttcgg gtctcagtcg aaccacttcc agacttccca
      121 acacgcggcc tctttcgtct cttccgcggc cggggcggcg tccgtcgcag ccgccgcggt
      181 ctgcgccggc gccgccgcgg cgccttgcgg gccgatgccg cccgagctcg catcgggcgc
      241 gacgccgtcg gccgagaagt cgacgaacgt gagctccgtg cacttgtcgg gcgagtacgg
      301 cagatccgca acgccgatcg tgtcgcttcc tgagtactga tccgtgtagt agacgggatc
      361 ggtcgccgtg tactcgcgcg tgaccgtcat cgcgttagga tcgagggaga agcgctcgac
      421 gacgtgcagc tgctcgctgt tcttgatggg cgggttcaga tagccctctg tgaacccgat
      481 cgtgtcgacg acgagcacgt cgttctccca gcggccgatc gaatgcccgg cgcggctcgg
      541 ctcgatgttg gccgggtgct cggcgttcat gtggatcgtg cgcgtgaagc cgagctggcc
      601 gtactgcaac gtgatcgtgt cgccgttctg cgtgatgcga ttgacggggc cgtcgaacgt
      661 ccagtcgaac aggatgctcg tcgtctcgca acgcatgcgc gggttgtcgg tcgtgccgcc
      721 gcggaacgcg tcggcggccg ccttgcccgc ctcggtgtag gtgacgcgcg ggcgcccgcg
      781 attgccgccc gcgccggccg gcggcgcggt cgcgccgggc tcgaagtcgt cgacgcggct
      841 cacgggcacg agcgcgccgc tgcgtccgcg cggatcagtc atgacgactt gctcgggcgc
      901 ccaatcgccc gtgatgttcg gctcgccgct tgcggtgcgc gctgcgcgag gcgccgcgga
      961 cggcgctgct tgcgtgggct tcgacaactg cgcgtaacgg tcggccgtcg tgccgtcggc
     1021 gaacaccacg gtgtgcacgt agcacgagtt cacgtcgaac cgatcgggcg cgccctggat
     1081 cttgatcgtc tcgcccgtct tgaacatctc ggccgaccag ccggaacggc gcagcaccgt
     1141 ggcggcgcgc atctcgcagc gcttcgcgac cttcgtaccg tcgggattcg tcgcctcgaa
     1201 gtagacgtag gcgtgcggat tcacgaaatc gatgctcgtg atagtgccct cgagcgcaac
     1261 ctcgcggctg cggtcgaaat tgccgaagcc gtgatgagcg cttgcggcgc cggcggccag
     1321 cagggccgca cacgcggctg cgaggcgcgt tggcgtgacg ttcatgttcc ccccggaaaa
     1381 gtcgacctgt ttaggttgtt cgagcttttc cggtattaat ccccaacttc agggcttgct
     1441 gcaaccggaa acgccattgc tcccaagacg gttgcccgcc cttttgagca ggcttatcat
     1501 gagcatgctt actatttcga gggcttgatg cctcccgcag ttttcgtcga tcgcacgctg
     1561 ggcttcctcg tagcggacat cgcccggctg ctcaggcgca gcatggaccg gcgcctgcaa
     1621 tcgctgggcc taacgcaggc gcagtggcgc gccatcgtgc acttgtcgcg cagcgaaggg
     1681 atgacgcagg ccgcgctcgc cgagagcctc gagattgcgc cgatcacgct cacgcgcctc
     1741 atcgaccgca tggaaagcgc gggctgggtc gagcggcgca cacatccgct cgatcgccgc
     1801 gccgtgcagc tctatctgac ggcgcaatcg cagccgatcc tcgagcagat gcatgcacgc
     1861 gccgccgata cgctgaacga ggcgatgcgc ggcatctcgc cgcgcgcgca gcgccagctc
     1921 gtcgcgtcgc tcgagcagct caagcacaac ttgatcgccg cggaaacggc ggcggtctcg
     1981 ccggaaccat caggaattcg tcatggccga ccaagactcg caaaatccgc ccgcgcccgt
     2041 gtctgacgcc gcgcccccca ctccggcaac agccgcgcga ccacggcggc ggctccgccg
     2101 cgtcttgctc ctgttggggc cgatcgtcgc gctcgctgtc ggcggctact tctattacac
     2161 gtcgggccgc atcgtcggca ccgacaatgc ctacgtcaaa tcggacgtcg gcgtcgtcag
     2221 cgcggaggtt gcggggccga tcgccgcgct gaacgtgcac gagaacgacc gcgtcgaggc
     2281 cggcgaggtc ctgttcacga tcgacgatcg cccgtttcgg gtcgcgctcg accgcggcaa
     2341 cgcgcagctc gcggcgatca aggacttcgt cgagagcacg aaggcgtcgt atcggcaatc
     2401 gctcgagcag ctcgcgctcg cgcgcacgaa cgcggcctat gagcagcgcg agctcgaccg
     2461 gctcgccgcg ctcgccgagc gcaagctcgc gtcggatgtc gacgtcgacg agcaacgcca
     2521 cgaccgcgac gtcgcgaacc aggagattca agtcacggag cgcgcgctcg atgcgattcg
     2581 cgcgcgcctc ggcggcgatc tcgatcgtcc cgtcaccgag caggccgcgt acttggccgc
     2641 gaaatcgatg cgcgacgcgg ccgagctcga tctcgaccgc acggtcgtgc gcgcgccgtt
     2701 cagcggaatc gcgagccaag tgccgacggt gggccaccac gtgcagcccg gggcgccggt
     2761 catgacgatc gtggccgatc gcgacatgtg gatcgaagcg aatttcaagg agaccgactt
     2821 gacgcacgtc
//
LOCUS       DASSNN010000134         3296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_405925, whole genome shotgun sequence.
ACCESSION   DASSNN010000134 DASSNN010000000
VERSION     DASSNN010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405925"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFL84_02390"
     CDS             <1..544
                     /locus_tag="VFL84_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607499.1"
                     /translation="DERMSIDLGGIEVVMFYLGPTHTDNLIQVHIPAEKVLIAVDFVR
                     EGKSLAMPELRDLNLDNSIRALGLLGRMENVDIVVPGHGGITTQQSFIYVRDFLAALK
                     ERVLAEMVAGKGIEDILKTVTMEDFSDYGWFHQWIRANVITMWELMYHYREPTLDPGC
                     YECDFPIGFPVGEVDKFNGP"
     gene            684..1436
                     /locus_tag="VFL84_02395"
     CDS             684..1436
                     /locus_tag="VFL84_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607500.1"
                     /translation="MRSIFFGAAMLALAAGCTSRESVDHPDFSGIYRPAGLEVQPCGR
                     NEWMRRSFETDTFCNGDDSAFPFTEAGRQRWKTYNVIDDPVLGCVEDFPRNAMRGRPM
                     KITIGEDVTEIAYWFNNEWFVRTVHMNGAPPAADTPNTVTGYSTGHWVGDTLVVETTH
                     TRGGPMYNDHKPNSTAAKFTERFWRAPGGQNLLMDIAIDDPEVYTKPFLLNRQEWLAT
                     GPDYKLSEDKCEPSSIWERRMRAKAQAAETGK"
     gene            1501..2109
                     /locus_tag="VFL84_02400"
     CDS             1501..2109
                     /locus_tag="VFL84_02400"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7607501.1"
                     /translation="MRHVIPCCAAVAATFVAGAAAAHHSDSLYFVDDRSADGGAIRIE
                     GTISRVRWINPHSEFFVDVPSPTGGEPAVWAIETDSVNQLRGLGWNDKTLEVGDKVVV
                     VVSKSKFDDTAGRLRDMLVYGSAPDEPASVYLEFKGDGKPEWAAPYEVYKKYTPCPGT
                     VPFDAERQPGKETMLCAHLSAAELAAAKRDYRARVLLLRDPQ"
     gene            2303..>3296
                     /locus_tag="VFL84_02405"
     CDS             2303..>3296
                     /locus_tag="VFL84_02405"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET7607502.1"
                     /translation="MRIYDLRLAALSAAVVGCLPFGASAQGQLEEIVVTATRRETDLQ
                     STPLSIQAFTAEQLELGGITNGRDLGIMVPNVVLNPSTGGAQSQFYVRGLPGVGLYVD
                     GIWQDGFGFQQMNFSEFERVEVLRGPQGTLFGRNTNGGAVNMTTRKPADEFGARIKLD
                     VGDFNRRDAQLAVDLPLTDNLKTKFMGASFKNDGFLEGLTTPWDFGAQDDQLLRADIL
                     WEATDSFSLRFTYNDEQKRGTDPRIHRMTRYDGSRVYAYNIMLGAFQAEANAACAADP
                     ARCQAIGATGNAIPVITTNQTTGQRTQTTSQSANFAGPGGTSWLPPPNIGVGTRY"
BASE COUNT          629 a         1056 c         1047 g          564 t
ORIGIN      
        1 cgacgagcgc atgtcgatcg acctcggcgg catcgaggtc gtgatgttct acctcggccc
       61 aactcacacg gacaacctga ttcaagtgca cattccggcc gagaaagtgt tgatcgccgt
      121 cgatttcgtt cgtgaaggca agagcctcgc gatgccggag ctgcgcgact tgaatctcga
      181 caactcgatt cgcgcgcttg ggcttctcgg gcgcatggag aacgtcgata tcgtcgtgcc
      241 cggccatggc ggcatcacga cgcaacaatc gttcatctac gtgcgcgact tcctcgccgc
      301 gctgaaagag cgcgtgctcg cggagatggt cgcgggcaag ggtatcgaag acattctgaa
      361 gaccgtgacg atggaagact tcagcgacta cggctggttc catcaatgga ttcgcgcgaa
      421 cgtcatcacg atgtgggagc tcatgtacca ctaccgcgag ccgaccttgg acccgggctg
      481 ctacgaatgc gatttcccga tcggtttccc cgtcggcgag gtcgacaagt tcaacggacc
      541 ctagctcgcg cgcacgatgg gctggatctg actgccccgg ccggtgctcg taggaccgaa
      601 cggttcgttc gcgggcgcgt tgccgcttgc caaaatctac gcaagaatgc cgagtactgc
      661 gcatgccatg ggggcttgtt ccagtgcgaa gcatcttctt cggcgccgca atgcttgcgc
      721 tcgcggcagg gtgcacgtcg cgcgagagcg tcgaccatcc cgatttctcg ggcatttacc
      781 gccccgccgg cctcgaggtg caaccgtgcg gccgcaacga gtggatgcgc cgctcgttcg
      841 aaaccgacac gttctgcaac ggcgacgaca gcgcgttccc gttcacggaa gcggggcgcc
      901 agcgctggaa aacgtacaac gtcatcgacg accccgtgct cggctgcgtc gaagatttcc
      961 cgcgcaacgc gatgcgcggc cggccgatga agatcacgat cggcgaagac gtcaccgaga
     1021 tcgcgtactg gttcaacaac gagtggttcg tgcgcaccgt gcacatgaac ggcgcgccgc
     1081 cggcggccga cacgccgaac accgtcaccg gttactcgac gggccactgg gtcggcgaca
     1141 cgcttgtcgt cgagacgacg cacacgcgcg gcggcccgat gtacaacgat cacaagccga
     1201 acagcacggc cgcgaagttc acggagcggt tctggcgcgc gccgggcggc caaaacctgc
     1261 tcatggacat cgcgatcgat gaccccgagg tgtacacgaa gccgttcttg ctgaaccggc
     1321 aggaatggct cgcgacggga cccgactaca agctcagcga agacaaatgc gagccgagct
     1381 cgatctggga gcggcgcatg cgcgccaagg cacaagctgc cgagaccggc aaatagaacg
     1441 cgacgccggc agggctgccg gcggtagggt aatgcaggga gcaattgccg aggagaggtc
     1501 atgaggcacg tcattccgtg ttgtgcagcg gtcgccgcca cgttcgtcgc gggtgcagcc
     1561 gcagcgcacc attccgattc gctgtacttc gtcgacgacc gctcggccga cggcggcgcg
     1621 atccgcatcg aaggcacgat ctcgcgcgtg cgctggatca atccgcactc ggagttcttc
     1681 gtcgacgtgc cgagccccac cggcggcgag ccggccgtgt gggccatcga gaccgattcc
     1741 gtgaatcagc tgcgcggcct cgggtggaac gacaagacgc tcgaagtcgg cgacaaggtc
     1801 gtagtcgtcg tctcgaagtc gaagttcgac gacacggccg ggcgtttgcg cgacatgctc
     1861 gtctacggct cggcgccgga cgagccggcg tccgtctatc tcgagttcaa aggcgacggc
     1921 aagcccgagt gggcggcgcc ttacgaggtc tacaagaaat acacgccgtg cccgggaacg
     1981 gtgccgttcg atgccgagcg gcagcccggt aaagagacga tgctctgcgc gcacttgagc
     2041 gcggccgagc ttgcggccgc gaagcgcgac tatcgcgcgc gcgtgctgct gcttcgcgac
     2101 ccgcagtaag aacgaaaacg ccgtcgcgcc gaaccgctcg gtctcgtctt cggcccgacc
     2161 ggttcttgtt ggttcagggc aacgaaatcg cgcaaacccg cgtggaatga ggttgacagt
     2221 agtgtttcca ctaacacgat cttagtccat actccggtgg ggcttcgtta ccgcgcgcgt
     2281 tttttctgag ggggaatttc gcatgcgtat ctacgatctg cgcttggctg cgttatctgc
     2341 ggccgtcgtc ggctgccttc cgttcggtgc ttccgcgcaa ggtcaactag aagaaatcgt
     2401 cgtcactgcc actcgccgcg aaaccgatct acaaagcaca ccgctctcga ttcaagcgtt
     2461 cactgccgag caactggagc tcggcggcat cacgaacggc cgcgacctcg gcatcatggt
     2521 gccgaacgtc gtgctgaacc cgagcacggg cggcgcgcaa tcccagttct atgttcgcgg
     2581 cttgccgggc gtcggtttgt acgtcgacgg catctggcaa gacggcttcg gcttccagca
     2641 gatgaacttc tcggagttcg agcgcgtgga agtgctgcgt ggtccgcagg gcacgctgtt
     2701 cggccgcaac acgaacggcg gcgccgtcaa catgacgacg cgcaaacccg ccgacgagtt
     2761 cggcgcgcgc atcaagctcg acgtcggcga cttcaatcgc cgcgacgcgc agctcgccgt
     2821 cgacctgccg ctgacggaca acttgaagac gaagttcatg ggcgcgagct tcaagaacga
     2881 cggcttcctc gaagggttga cgacgccgtg ggacttcggc gcgcaagacg accagctgct
     2941 gcgcgcggat atcttgtggg aggcgaccga ctcgttctcg ctgcgcttca cgtacaacga
     3001 cgagcagaag cgcggtaccg atccaaggat ccaccgcatg acgcgctacg acggctcgcg
     3061 cgtgtacgcg tacaacatca tgctcggcgc gttccaggcc gaggcgaacg ccgcgtgcgc
     3121 ggcggatccg gctcgctgcc aagccatcgg cgctacgggt aatgcgattc ccgtcatcac
     3181 gaccaatcaa acgacgggcc agcgcaccca gacgaccagc cagagcgcca acttcgccgg
     3241 ccccggcggc acttcttggt tgcccccgcc gaatatcggc gtcggcacgc gctaca
//
LOCUS       DASSNN010000135         4349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1414707, whole genome shotgun sequence.
ACCESSION   DASSNN010000135 DASSNN010000000
VERSION     DASSNN010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414707"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1755)
                     /locus_tag="VFL84_02410"
     CDS             complement(40..1755)
                     /locus_tag="VFL84_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7607503.1"
                     /translation="MGSVALNFLGDLTIARDGEPLELPPSRKTRALLAYLALTGRAFR
                     REHLCELLWEIPDDPRGSLRWSLSKLRRLVDDEARERIVADRLSVRFDATDVSVDVAA
                     LASIAQGKLDEAPLDELEAAAARYCGTPLEGLELPTFHDYSAWYLGERERATANQVRL
                     LNALVKRLAGDPQRAVEHARTLVRIAPYDEKARAALIRLLMAIGRPDQAEQHYQMGSR
                     LLKEAGATPTGELFRAWRGAPGTSTPASPTPAATTPAARATQRPRVAQDTKVAPETQL
                     IGRDAELERVRAAMTAAFAERRCRAVLLVGEPGIGKSRLLEAAAGLARDAGALVLEAA
                     AYEAESIRPFALFVDALRKLEPAAAAAVFERGDSSNRDRLFERLAEIVAARAAAQPVV
                     LKFDDLQWSDESSAAALHYVVRTSAALNVLVLLAARRDELRDNTPILRVVRELRHLGL
                     LEELDLAALGEHAVREIIGARAPQADAERLCKECGGNPLLAIELARAEVAGESGASLR
                     EVIQERLARFDADGGEVLRWAAVLSPRIDAASIRGDSTAAQRSTSPPSASKRARRSWM
                     TSRSD"
     gene            1914..3419
                     /gene="cysK"
                     /locus_tag="VFL84_02415"
     CDS             1914..3419
                     /gene="cysK"
                     /locus_tag="VFL84_02415"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008885043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET7607504.1"
                     /translation="MARYHNILETIGNTPLVKLGKLAPPGVNVYVKVESRNPMGSVKD
                     RMARAIIERAEASGALKPGQTVIEATSGNTGIGLAMVCAQKGYPLVVTMAESFSVERR
                     KLLRFLGAKVVLTPASEKGSGMLAKAVELAETHGWFLCRQFENEANADVHTRTTAQEI
                     LADFAGERLDYWVSGFGTGGTLKGVARALKQASPVTRVVAAEPDNAPVLASGIPQPRD
                     ASGKPSQSHPNFRPHLMQGWAPDFISKLTEDAVAAGLVDEIVPVAGNDALKLARDLAR
                     REGIFVGTSSGATLAAALTIARRSKPGTNIVCMLPDTGERYLSTPLFADIGEEMTAEE
                     LTLSRSTPSCRFDTPAPTLAPAPVSPVTAPAPVVLDAEAERFFDHVVRHEPVVLFALE
                     WCEFSWSVRKLFARLGIAYRSVDLDSVALQGNDLGGKIRAVLGTRTGTKTIPQVFIGG
                     THIGGATEMFDAWRNGKVRELLSARGVAFDAQADLDPYSLLPKWLQPRKSA"
     gene            3433..>4349
                     /locus_tag="VFL84_02420"
     CDS             3433..>4349
                     /locus_tag="VFL84_02420"
                     /inference="COORDINATES: protein motif:HMM:NF015150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HET7607505.1"
                     /translation="MSTATLPVIDVSDLRRAATHREVDAACREWGFFQIVGHAIADRT
                     LDALRRQMRAFFALATTSKHAIMRTAENPWGFYDRELTKRTRDWKQVFDYGPPDGGAI
                     VPQLPRELPRFGSAVARFYAECDALAFELVEVIAGNLGTPPGALDSCFRPDHTSFLRL
                     NYYPPCPLPARPAVGPTAGHLGVNAHTDSGALTLLLQDDQPGLEVFHDGAWQLVEPRR
                     DALVVNIGDIVQVWSNDRYTAALHRGLVSANAERFSAPFFFNPAYRTWYAPLLSTVDA
                     GHPARYRPIHWGEFRARRAAGDYADHGEY"
BASE COUNT          659 a         1564 c         1495 g          631 t
ORIGIN      
        1 gatcgagctc gcgcgcgccg aagtggccgg cgagagcggt cagtcgctgc gcgaggtcat
       61 ccaggaacgc ctcgcgcgct tcgacgccga cggcggcgag gtgctgcgtt gggccgccgt
      121 gctatcgccg cgtatcgacg ccgcgtcgat gcgcggcgac agcacggcgg cccagcgcag
      181 cacctcgccg ccgtcggcgt cgaagcgcgc gagccgctcc tggatgacct cgcgcagcga
      241 cgcgccgctc tcgccggcaa cctcggcgcg cgcgagctcg atcgcgagca gcggattgcc
      301 gccgcactcc ttgcagagcc tctcggcgtc ggcttgcggc gcgcgcgcac cgatgatctc
      361 gcgcacggcg tgctcgccga gcgccgcaag gtcgagctcc tcgagcaacc ccaagtgccg
      421 cagctcgcgc acgactcgca agatcggcgt gttgtcgcgc agctcgtcgc ggcgcgccgc
      481 gagcagcacg agaacgttca aagcggcgct cgtgcgaacg acgtaatgca gcgcggccgc
      541 gctcgactcg tcgctccact gcaagtcgtc gaacttgagc acgacgggtt gtgcggccgc
      601 gcgggcggcg acgatctccg cgagccgctc gaacagccgg tcgcggttcg acgagtcgcc
      661 gcgctcgaag acggccgccg cggcagccgg ctcgagcttg cgcagcgcgt ccacgaacaa
      721 ggcgaacggc cggatcgact ccgcttcgta agcggccgcc tcgagcacga gcgcgccggc
      781 gtcgcgcgcg agacccgcgg ccgcctcgag caagcggctc ttgccgatgc cgggctcgcc
      841 gacgagcagc acggcgcggc agcggcgttc cgcgaacgcg gccgtcatcg cggcacgcac
      901 gcgctcgagc tcggcatcgc ggccgatcag ctgcgtctcg ggcgcgactt tcgtgtcttg
      961 cgcgacgcgc ggacgttgcg ttgctcgagc agccggcgtc gtagcagcgg gggtagggga
     1021 ggccggcgtt gaggtgcccg gggcgccgcg ccaggcgcgg aacagctcgc cggtcggcgt
     1081 cgcgcccgct tccttcagca accgcgagcc catctgataa tgctgctcgg cctggtcggg
     1141 ccggccgatc gccatcaaca accgaatcag cgccgcgcgc gccttctcgt cgtaaggcgc
     1201 gatgcgcacg agcgtgcgcg cgtgctcgac cgcgcgttgc gggtcgccgg cgagccgctt
     1261 cacgagcgcg ttcaacagcc gcacttggtt cgccgtcgct cgctcgcgct cgcccaagta
     1321 ccaggccgag taatcgtgga acgtcggcag ctcgaggcct tcgagcggcg tgccgcagta
     1381 gcgcgccgcg gccgcctcga gctcgtcgag cggcgcttcg tcgagcttgc cttgcgcgat
     1441 cgaagcgagc gcggcgacgt ctacgctcac gtcggtcgcg tcgaaccgca cgctgagccg
     1501 gtccgcgacg attcgctcgc gcgcttcgtc gtcgacgagg cgccgcagct tcgacaagct
     1561 ccagcgcagc gagccgcgcg gatcgtcggg aatctcccag agcagctcgc agaggtgctc
     1621 gcggcggaac gcgcggcccg tcagcgcgag ataggctagc agggcgcgtg tcttgcgcga
     1681 gggcggcagc tcgagcggct cgccgtcgcg agcgatcgtc agatcgccga ggaaattcag
     1741 cgccacgctc cccatggagg gccgattgta ggcttctgcc cgagctccct ccaacgcgcg
     1801 ccagccgcta ccacggcgcc tcgccccaaa aaaccacgct ttctcacacg ggcattgcca
     1861 cggtcggcgg ccaacatggc ctccacgacg aaccgctgtg acaggagaga ccgatggccc
     1921 gctaccacaa catcttggag acgatcggca acaccccgct cgtgaagctc ggcaagctcg
     1981 ccccgcccgg cgtcaacgtt tatgtcaagg tcgagtcgcg caacccgatg ggctcggtca
     2041 aagaccgcat ggcccgagcc atcatcgagc gcgccgaagc gtcgggcgcg ctgaagccgg
     2101 gacaaaccgt catcgaagcc acgagcggca acaccggcat cgggctcgcg atggtctgcg
     2161 cgcagaaggg ctatccgctc gtcgtcacga tggccgagag cttcagcgtc gagcggcgca
     2221 agctgctgcg cttcctcggc gccaaggtcg tgctgacgcc ggcgtccgag aaaggcagcg
     2281 gcatgctcgc gaaagccgtc gagctcgccg agacgcacgg ctggttcctg tgccgccaat
     2341 tcgagaacga agcgaacgcc gacgtgcaca cgcgcacgac ggcgcaagag atcctcgccg
     2401 acttcgccgg cgagcgtctc gactactggg tctcgggctt cggcacgggc ggcactctga
     2461 aaggcgttgc gcgcgcgctg aagcaggcga gccccgtcac gcgcgtcgtc gccgcggagc
     2521 ccgacaatgc gcccgtgctc gcgagcggga ttccgcagcc gcgcgacgcg agcggcaagc
     2581 cgagccagag tcatccgaat tttcgcccgc acctgatgca gggctgggca ccggatttca
     2641 tttccaagct cacggaagac gccgtcgctg cgggcctcgt ggacgaaatt gtgcctgtcg
     2701 cgggcaacga cgcgctgaag ctcgcgcgcg acctcgcgcg gcgcgaaggc atctttgtcg
     2761 gcacgtcgag cggcgcgacg ctcgcggccg cgttgacgat tgcgcgccgc tcgaagcccg
     2821 gcacgaacat cgtctgcatg ctgcccgaca cgggcgagcg ctatctctcg acgccgctgt
     2881 tcgcggacat cggcgaggag atgacggccg aggagcttac gctatcgcgc tcgacgccgt
     2941 cgtgccgctt cgacacgccc gcgccgacgc tcgcgccggc gcccgtaagc cccgtgaccg
     3001 cgcccgcgcc ggtcgtgctc gacgccgaag cggagcgctt cttcgaccac gtcgtgcgcc
     3061 acgagcccgt cgtgctgttc gcgctcgagt ggtgcgagtt cagctggtcc gtgcgcaagc
     3121 tgttcgcgcg gctcggcatc gcgtaccgca gcgtcgatct cgactccgtc gcgctgcaag
     3181 gcaacgacct cggcggcaag attcgagccg tgctcggcac gcgtacgggc acgaaaacga
     3241 tcccgcaggt cttcatcggc ggcacgcaca tcggcggcgc caccgaaatg ttcgacgcct
     3301 ggcgcaacgg caaagtgcgc gagctgctct cggcgcgcgg cgttgcgttc gatgcgcaag
     3361 ccgatctcga tccgtactcg ctgctgccga aatggctgca gccgcgcaag agcgcgtgag
     3421 cggaacgcgc acgtgagcac cgcaaccttg cccgtcatcg acgtcagcga cttgcggcgc
     3481 gcggcaacgc accgcgaggt cgacgccgcg tgccgcgagt ggggcttctt tcagatcgtc
     3541 ggccatgcca tcgccgaccg cacgctcgac gctctgcgcc ggcagatgcg cgcgttcttc
     3601 gcgctggcga ccacctcgaa gcacgcgatc atgcgcacgg ccgagaaccc gtggggcttc
     3661 tacgaccgcg agctgacgaa gcgcacacgc gactggaagc aggtcttcga ctacggtccg
     3721 cccgacggcg gcgcgatcgt gccgcagctg ccgcgcgagc tgccgaggtt cggctccgcg
     3781 gtcgcgcggt tctatgccga gtgcgacgcg ctcgcgttcg agctcgtcga ggtcatcgcc
     3841 ggcaatctcg gcacgccgcc gggcgcgctc gattcgtgct tccggccgga ccacacgagc
     3901 ttcttgcgcc tcaactacta cccgccgtgt ccgctgccgg cgcggcccgc ggtcgggccg
     3961 acggcggggc atctgggcgt caatgcgcac acggattcgg gcgcgctgac gctgctgctg
     4021 caggacgacc agccgggcct cgaggtgttt cacgacggcg cgtggcagct ggtcgagccg
     4081 cgccgcgatg cgctcgtcgt gaacatcggc gacatcgtgc aggtctggtc gaacgaccgc
     4141 tacaccgcgg cgctgcatcg cggtctcgtg tcggcgaacg ccgagcgctt cagcgcgccg
     4201 tttttcttca acccggctta ccgcacctgg tatgcgccgc tgttgtcgac cgtcgacgcc
     4261 gggcatcccg cgcgttatcg gccgattcac tggggcgagt ttcgcgcgcg gcgcgccgcg
     4321 ggcgactacg ccgaccacgg cgagtacca
//
LOCUS       DASSNN010000136         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1540781, whole genome shotgun sequence.
ACCESSION   DASSNN010000136 DASSNN010000000
VERSION     DASSNN010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540781"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFL84_02425"
     CDS             complement(<1..265)
                     /locus_tag="VFL84_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7607506.1"
                     /translation="MQLFRRPYRLACCAAPPLAVGTAALAQEPAKTRVPLEEIVVTAT
                     LLERTLDKTPAAVSVVTEDDIQRARQQLGLDESLNRVPGLFMQN"
     gene            437..1072
                     /locus_tag="VFL84_02430"
     CDS             437..1072
                     /locus_tag="VFL84_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838627.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HET7607507.1"
                     /translation="MQFVSAAEMVELVEVVFVNLVLSADNAIVVGIAVAGLPARNRLR
                     AMALGIAGATVLRILFALVTTELLSVVGLKLAGGVLLLWICSKLWRELRTGQLIDSQA
                     PHEVVYDPDAPASAAPPKTMQQAMLQIVVADVSMSLDNVLAVAGVAGQHWSVLVIGLV
                     LSIVFMGLAAALIARLLGRYHWIAYVGLAIIVYVSLRLILDGTLELVEAVG"
     gene            complement(1069..1530)
                     /locus_tag="VFL84_02435"
     CDS             complement(1069..1530)
                     /locus_tag="VFL84_02435"
                     /inference="COORDINATES: protein motif:HMM:NF021049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HET7607508.1"
                     /translation="MKDRAALCAELTATWHREIPIVAAMGAAVESYDGRSLAVRAPMQ
                     PNRNVHGTTFAGSLYSTCVLTGWGAVWLALRERGLDAVIYAAESAVQYRKAVAGDFVC
                     RCTFDAAALEAELEQFKESGRASFDLACTIDHGGKRAVAFTGKYVIHAKRS"
     gene            1663..>2259
                     /locus_tag="VFL84_02440"
     CDS             1663..>2259
                     /locus_tag="VFL84_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7607509.1"
                     /translation="MLQVQSKLALCSFPLALLAAGSALAQGPQQDFSAVQIKTHEVVG
                     NFYYLEGQGGNVGVLVGDDGVLMIDDQFAPLSEKLQAAIKALSSKPIRMLVNTHVHGD
                     HTGGNENFGKLGVDIVAHDNVRVRLAKPATGAPAPAVALPVVTYGDKMSLHLNGETIT
                     IGKLPPAHTDGDSYIHFAKADVIHAGDVFRTTGYPGVDA"
BASE COUNT          367 a          739 c          758 g          395 t
ORIGIN      
        1 ggttctgcat aaacaggccc ggcacgcggt tcaatgactc gtccagcccg agttgctggc
       61 gcgcgcgctg aatatcgtct tctgtcacga cgctgacggc ggccggggtc ttgtcgagtg
      121 tgcgttcgag caaggttgcc gttacgacga tctcctcgag cgggactcta gtcttcgccg
      181 gctcttgggc aagcgcagcg gtgccgaccg ccaggggagg ggccgcgcaa caagcaagac
      241 ggtagggccg gcgaaatagc tgcatggatg atcccgtggg tgattcgagg cctgattggc
      301 cgggtatcat gacccagtga gaccttaggc ggcaacggcg cccgatcggc aagttgacgt
      361 ggcggctgcg agacaggcgt gaattgaagg ggccttccgg gcacactggc ggccccgcgc
      421 agtcaagacc agccgcatgc agttcgtgtc agccgccgag atggtggagc tcgtcgaggt
      481 cgtgttcgtg aacctcgtgc tctccgccga caacgcgatc gtcgtgggca tcgcagtggc
      541 gggccttccg gcgcgcaatc ggcttcgcgc gatggcgctt ggcatcgccg gtgccacggt
      601 gctgcgcatc ctgttcgcgc tcgtgacgac ggagctgctc agcgtcgtcg gcttgaagct
      661 cgccggcggc gtgttgctgc tttggatctg ctcaaagttg tggcgcgagc tgcgcaccgg
      721 tcagctgatc gactcgcagg cgccgcacga ggtggtgtac gaccccgacg cgccggcctc
      781 ggcggcgccg ccgaagacga tgcaacaagc catgctgcag atcgtcgtgg ccgacgtctc
      841 gatgtcgctc gacaacgtgc tcgccgtcgc cggcgtcgcg ggccagcact ggtctgtgct
      901 cgtgatcggg ctcgtgcttt cgatcgtgtt catgggcctc gcggcggcgc tcatcgcgcg
      961 gctgctcggc cggtatcact ggatcgccta cgtcgggctc gcgatcatcg tgtacgtctc
     1021 gttgcgcctg atcttggacg gcacgctcga gctcgtcgaa gccgtgggct aagaccgctt
     1081 cgcgtggatg acgtacttgc ccgtgaaagc gacggcacgc ttgccgccgt ggtcgatcgt
     1141 gcaagcgaga tcgaagctcg cgcgccccga ctctttgaac tgctcgagct cggcctccaa
     1201 cgccgcggca tcgaacgtgc agcggcagac gaaatcgccc gcgacggctt tgcgatattg
     1261 aacggcgctc tcggcggcgt agatgacggc gtcgaggccg cgctcgcgca acgcgagcca
     1321 aacggctccc caacccgtca gcacgcacgt cgaatacaag ctgcccgcga acgtcgtgcc
     1381 gtgcacgttg cggttcggtt gcatcggcgc acgcacggcc agcgaccgcc cgtcgtagct
     1441 ctcgaccgcg gctcccatgg ccgccacgat tggaatctcg cggtgccagg tcgcggtgag
     1501 ctctgcacag agggcggcgc ggtccttcat cggccgaagt atcgcacgct caggaaaacc
     1561 cgcgttgcgg tcgtgctgcc cgcggtgaga agccggtgct atcatcgctc gcaaagctgc
     1621 gcgctcacgg cgccggaacg acattcgctc tcggggggag tcatgctgca agtgcaatcg
     1681 aagctcgctc tttgctcatt cccgctcgct ctgctcgccg ccggctctgc tttggcgcag
     1741 gggccgcagc aggacttctc ggcggtgcaa atcaagacgc acgaagtcgt cggcaacttc
     1801 tattacctcg aggggcaggg cggcaacgtc ggcgtgctcg tcggcgacga cggcgtgctc
     1861 atgatcgacg atcagttcgc gcctctgtcc gagaagctgc aggccgcgat caaggcgttg
     1921 tcgagcaagc cgattcgcat gctcgtcaac acgcacgtgc acggcgatca cacgggcggc
     1981 aacgagaact tcggcaagct cggcgtcgac atcgtcgcgc acgacaacgt gcgagtgcgc
     2041 ctcgcgaagc ccgccaccgg cgcgccggcg ccagccgtgg cgctgcccgt cgtcacgtac
     2101 ggcgacaaga tgtcgctgca cttgaacggc gaaacgatca cgatcggcaa gctgccgccg
     2161 gcgcacacgg acggcgacag ctacatccac ttcgcgaagg ccgacgtcat tcacgccggc
     2221 gacgtgttcc gaacgacggg ctaccccggc gtggacgcc
//
LOCUS       DASSNN010000137         2991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_532704, whole genome shotgun sequence.
ACCESSION   DASSNN010000137 DASSNN010000000
VERSION     DASSNN010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532704"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="VFL84_02445"
     CDS             <1..1319
                     /locus_tag="VFL84_02445"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET7607510.1"
                     /translation="GGCDKNMPGCVMAMARLDRPSIFVYGGTIRPGPKDRDIISVFEA
                     VGSYAAGKIDDAELKEVESTAIPGPGSCAGMYTANTMASAVEALGMSLANSSAQEAVS
                     SAKADDCRRAGEAVVALIKRGIKPSDILSKEAFENAITTVIALGGSTNAVLHLLAIAH
                     EARVDLTLADFTRIGNRTPVLADLRPSGRYMMSELIRIGGIQPLQKTLLDAGMLHGGC
                     FTVTGRALAENLAAVRPYPAGQTIVRPLSDPIKADSHIAVLYGNLAPEGAVAKITGKE
                     GTRFEGRARVFDDEYAALRAIIDGVVVAGDVIVIRYEGPKGAPGMPEMLSPTGAVIGR
                     GLGSKVALITDGRFSGGSHGFVVGHVTPEAAVGGPIAVVEDGDPIVIDAVRKTIELGV
                     SKETLDRRLRAWKPAPKKAERGLLAKYARLVSSASQGAVTDASLGE"
     gene            1359..>2991
                     /locus_tag="VFL84_02450"
     CDS             1359..>2991
                     /locus_tag="VFL84_02450"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET7607511.1"
                     /translation="MSSQPVLGASQAVDTPYPDSPWVVLKFGGRSVSTAENWGIVARL
                     LRERLAEGVRPVVVHSALVGVSNALIALLDAAVAGGDTDDKIAKIRAQHDALARELGV
                     DAKMFDERFASLAQVVAGTKLVGEVSPRVHARVLCTGELAATELGAAYLRKAGLDVTW
                     LDIREYLESTTLRDQTERARFISARCDFAPDKALQARFAAIDGIVLSQGFIARNAQGE
                     TVLLGRGGSDTSGAYLAGKLEARRLEIWTDAPGFFSADPKAVPSARLIKSLHYREAQE
                     IASAGGGILHPRSISPVRASGIPLYLKCTAHPEWEGSVISNAPGDDQPQLKAISHRTG
                     ITLVSMESMEMWHQVGFLADAFNIFREHGISVDLISTSESNVTVSIDTAQNVTTPAAI
                     ESLAADLRKLCKVTIIGDCAAITLVGQRIRTILHEIAPVLEVFQEQQVHLVTQAANDL
                     NLTFVVSSEHAYRLVQHLHGLLVDKFAGGVFGETWERLTGGGAPAKAAPKPWWAKKKA
                     QLLEIGAAHAAAYVYDRDSIEAAIASLKSLRAVDAV"
BASE COUNT          490 a         1008 c         1043 g          450 t
ORIGIN      
        1 tcggcggttg cgacaagaac atgccgggct gcgtcatggc gatggcgcgg ctcgaccggc
       61 cgtcgatctt cgtgtacggc ggcacgatcc ggccgggccc gaaggaccgc gacatcatct
      121 cggtcttcga ggccgtcggc agctatgccg cgggcaagat cgacgacgcc gagctcaagg
      181 aggtcgagtc gacggcgatc ccggggcccg ggtcctgcgc cggcatgtac acggcgaaca
      241 cgatggcgtc ggcggtcgaa gcgctgggca tgagcctcgc gaacagctcg gcgcaggagg
      301 ccgtgtcgag cgcgaaggcc gacgactgcc gccgcgcggg cgaagccgtc gtcgcgctca
      361 tcaagcgcgg catcaagccg tcggacatcc tgagcaagga ggccttcgag aacgcgatca
      421 cgaccgtcat cgcgctcggc ggctcgacga atgccgtgct gcatctgctc gcgatcgcac
      481 acgaggcgcg cgtcgacctt acgctcgccg atttcacgag gatcggcaac cgcacgcccg
      541 tgctcgcgga cctgcggccg agcggacgct acatgatgtc cgagctgatc cggatcggcg
      601 gcatccagcc gctgcagaag acgctgctcg acgcgggcat gctgcacggc ggctgcttca
      661 cggtcacggg ccgcgcgctc gccgagaacc tcgcggccgt tcggccgtac ccggccggtc
      721 agacgatcgt tcggccgctg tccgatccga tcaaggccga cagccacatt gccgtgctct
      781 acggcaacct cgcgcccgag ggcgccgtcg ccaagatcac gggcaaggaa ggcacgcggt
      841 tcgaggggcg ggcccgcgtg ttcgatgacg agtacgcggc actgcgcgcg atcatcgacg
      901 gcgtcgtcgt ggccggcgac gtcatcgtga tccgctacga ggggccgaag ggcgcgcccg
      961 ggatgcccga aatgctgagc ccgaccggcg ccgtcatcgg ccgcggcctc ggcagcaagg
     1021 tcgcgctgat cacggacggc cgattctccg gcggcagcca cggcttcgtc gtcgggcacg
     1081 tgacgccgga agcggccgtc ggcgggccga tcgccgtcgt cgaggacggc gacccgatcg
     1141 tcatcgatgc cgtgcgcaaa acgatcgagc tcggcgtctc gaaggagacg ctcgatcgcc
     1201 gcctgcgtgc gtggaagccg gcgccgaaga aagcggagcg aggattgctc gcgaaatacg
     1261 cgcgcctggt cagctccgcg tcgcagggcg cggtgaccga cgcgtcgctc ggtgagtaga
     1321 caagaagtcc gaaaagcgaa gttcagaggc tgctctcaat gtcgtcacag ccagtgctag
     1381 gcgcgagcca agccgtcgat acgccgtatc ccgactcgcc ctgggtcgtg ctgaagttcg
     1441 gcgggcgcag cgtctccacg gcggagaact ggggcatcgt cgcgcgtctg cttcgcgagc
     1501 ggctcgcgga gggtgttcgc cccgtcgtcg ttcattcggc gctcgtcggt gtctcgaacg
     1561 cgttgatcgc gctgctcgat gccgccgtcg cgggcggcga caccgacgac aagatcgcca
     1621 agatccgcgc gcagcacgat gcgctcgcgc gcgagctcgg cgtcgacgcg aagatgttcg
     1681 acgagcgctt cgcgtcgctc gcgcaagtcg tcgccggcac caagctagtc ggcgaggtca
     1741 gcccgcgcgt gcatgcgcgc gtgctctgca cgggcgagct cgcggcgacc gagctcggcg
     1801 ccgcgtattt gcgcaaggcc ggcctcgacg tcacgtggct cgacatccgc gagtacctcg
     1861 agagcacgac gctgcgcgat cagaccgagc gcgcgcgctt catctcggcg cgctgcgatt
     1921 ttgcgccgga caaggcgctg caggcgcggt tcgccgcgat cgacggcatc gtcctgtcgc
     1981 aaggcttcat cgcgcgcaac gcgcaaggcg aaacggtatt gctcggccgc ggcggctccg
     2041 atacctcggg cgcttacctc gccggcaagc tcgaggcgcg gcgcctcgag atctggaccg
     2101 acgcgccggg gttcttcagc gccgatccga aggccgtgcc gtcggcgcgg ctgatcaaga
     2161 gcctgcacta ccgcgaagcg caggagatcg cgtcggccgg cggcggcatc ctgcacccgc
     2221 gcagcatctc gccggtgcgc gcgtcgggca ttcctttata tctgaagtgc acggcgcatc
     2281 cggagtggga aggcagcgtc atctcgaacg cccccggcga cgaccagccg cagctgaagg
     2341 cgatctcgca tcggaccggc atcacgctgg tctcgatgga atcgatggag atgtggcatc
     2401 aagtcggctt cctcgccgac gctttcaata tcttccgcga gcacggcatc tcggtcgacc
     2461 tgatctcgac gtcggagagc aacgtcacgg tgtcgatcga cacggcgcag aacgtcacga
     2521 cgccggcggc gatcgagtcg ctcgccgccg atctgcgcaa gctgtgcaag gtcacgatca
     2581 tcggcgactg cgcggcgatc acgctcgtcg gccagcgtat ccgcacgatc ctgcacgaaa
     2641 tcgcgcccgt gctcgaggtg tttcaggagc agcaagtcca tctcgtcacg caggccgcca
     2701 acgacctgaa tctcacgttc gtcgtcagct ccgagcacgc gtatcggctc gttcaacatc
     2761 tgcacggctt gctcgtcgac aagttcgcgg gcggcgtatt cggcgagacg tgggaacggc
     2821 tgacgggcgg cggcgcgccg gcgaaggcgg cgccgaagcc ctggtgggcg aagaagaagg
     2881 cgcagctgct cgagatcggc gcggcgcacg ccgcggctta cgtctacgat cgcgacagca
     2941 tcgaggcggc catcgcgtcg ctcaagtcgc tgcgcgcggt ggacgccgtg t
//
LOCUS       DASSNN010000138         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1036661, whole genome shotgun sequence.
ACCESSION   DASSNN010000138 DASSNN010000000
VERSION     DASSNN010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036661"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFL84_02455"
     CDS             complement(<1..207)
                     /locus_tag="VFL84_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607512.1"
                     /translation="MGRTRLLLRGGILVALSISLAACGGDEEEAAAVSDGNHAPTISG
                     TPAAAVSQGTSYSFTPAAADPDGDA"
     gene            complement(540..917)
                     /locus_tag="VFL84_02460"
     CDS             complement(540..917)
                     /locus_tag="VFL84_02460"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HET7607513.1"
                     /translation="MLFVYGTLRPFVDIEMARWLRGVARYLGPASTPGRLYDLGAYPG
                     MTAARSRRERVVGDVYRVANPRVLRVLDRYEASFMRERCVVKLERGARVTAWAYRYRH
                     RIGGATRIASGDYRAHWRGSIEA"
     gene            991..>2174
                     /locus_tag="VFL84_02465"
     CDS             991..>2174
                     /locus_tag="VFL84_02465"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HET7607514.1"
                     /translation="MRLSLFALTMVAAAAQARGVSPYLPVDLSPEIERKIERVLILAD
                     QPSLKRPFAAATVLDALPRACERDPVLCEDVRRFLTSLTRTAGISYATLTASGASGSS
                     TALPNRHGMASQSNYEAAIAAYWQPGNSFLVTGGVVSYDGETTPTGTVVSIGRDFAQL
                     DVGYRDHWWSPASDNAMLLSTQAATMPSVTISNYRPLTKAKLRYEAFVARMSESSNIA
                     FEGATTAGHPQLAGLHLSIEPLPGWSIGVARLMQYGGGDREESFSDLLDAFFNPSDED
                     NASPGSTDEFGNQVASLTSQLTVGDPLPMSIYFEYAGEDTSTLSNLRLGNTALSAGIA
                     FPQLGRRFAATFELTEWQNGWYVHHIYADGLRHMDHVLGHWGGDWRAVGDGVGALGAF
                     ARV"
BASE COUNT          367 a          740 c          713 g          354 t
ORIGIN      
        1 cgcgtcgccg tcggggtcgg ccgcggccgg cgtgaatgaa tacgaggttc cttggctgac
       61 ggccgcggcc ggcgtgccgg aaatcgtcgg tgcgtgattg ccgtcgctga cggcggccgc
      121 ctcctcctcg tcgccgccgc aagcggcgag cgagatcgag agcgcgacaa gaattccccc
      181 gcgaagcagc agccgcgtac gccccatacc tgaatcaccc ccagagacga cagggggctc
      241 atggcaactt tggtgccatg ccgagaaacg ctagggattc cgcggcctaa cacggttgag
      301 tctcgaaacg gagacaccat gaaccgtcgc tcttcggcga ccaatccgcg cagatccggt
      361 cgcgaccacg gaaaatatgg gtgtccggcg tcggcgaacc gcgacacggt gagtcgcttc
      421 acactgccga cggcgagaga ttcacggcga aaagcctgcg cggcccgtgc gtggcgcgcg
      481 gatacgcgcg gccgcgcgct cgtcgcgaaa cggagaccag cgacataacg ggacgtgttt
      541 taggcttcga tcgagccgcg ccaatgcgcg cggtaatcgc cgctcgcgat gcgcgtcgca
      601 ccgccgatgc ggtggcgata tcgataggcc catgccgtga cgcgcgcgcc gcgctcgagc
      661 ttcacgacgc aacgctcgcg catgaagctc gcttcgtatc gatcgagcac gcgcaagacg
      721 cgcggattcg cgacgcgata gacgtcgccg acgacgcgtt cgcggcggct gcgcgccgcg
      781 gtcatgccgg gatatgcgcc gagatcgtag agtcggcccg gcgtgctcgc gggaccgaga
      841 tagcgcgcaa cgccgcgcag ccacctcgcc atctcgatgt cgacgaatgg ccgcagcgtg
      901 ccgtagacga acagcaagga atcgcgtctc gtgggccttg acgatatcat gctggcctcg
      961 cgatcaatgg tagccggcgc tttgactctc gtgcgactgt ctctctttgc actgacgatg
     1021 gtcgccgcag ccgcgcaagc tcgcggagtc agcccgtatc ttcccgtcga cctctcgccc
     1081 gagatcgagc gcaagatcga gcgcgtgttg atcctcgccg atcaaccctc gttgaaacgg
     1141 ccgttcgccg ccgccacggt gctcgatgcg ctgccgcgcg cctgcgagcg cgatccggtt
     1201 ctctgcgaag acgtgcggcg ctttctcacg agcctcacgc gcacggccgg catcagctat
     1261 gcgacgctga ccgcgtccgg cgcctcgggc tcgagcaccg cgctgccgaa ccggcacggg
     1321 atggcctcgc aaagcaatta cgaagccgcc atcgccgcgt actggcagcc cggcaactcg
     1381 ttcctcgtga cgggcggcgt cgtgtcctac gacggcgaaa cgacgccgac cggaaccgtc
     1441 gtcagcatcg gccgcgactt cgcgcagctc gacgtcggct atcgcgatca ctggtggtcg
     1501 ccggcgtcgg acaacgcgat gttgctctcg acgcaagccg cgacgatgcc gtcggtcacg
     1561 atctcgaact accggccgct gacgaaagcg aagctgcggt acgaagcctt cgtcgcgcgc
     1621 atgagcgagt cttccaacat cgccttcgag ggcgcgacga ccgcgggcca tccgcagctc
     1681 gcgggcttgc acttgtcgat cgagccgctg cccggctggt cgatcggcgt cgcgcgcttg
     1741 atgcagtacg gaggcggcga ccgcgaagaa tcgttctccg atctcctcga cgcgttcttc
     1801 aacccttcgg acgaagacaa cgcttcaccg ggatccaccg atgagttcgg caatcaggtc
     1861 gcgtcgttga cctcgcagct caccgtcggc gatccgttgc cgatgtcgat ttacttcgag
     1921 tacgcgggcg aggacacgtc gacgctcagc aacttgaggc tcggcaacac cgcgttgtcg
     1981 gccggcatcg cgtttccgca gctcggccgc cggttcgcgg cgacgttcga gctcacggag
     2041 tggcagaacg gctggtacgt gcatcacatc tacgccgacg gcttgcgcca catggaccac
     2101 gtgctcggcc attggggcgg cgactggcga gcggtcggcg acggcgtcgg cgcgctcggc
     2161 gcattcgcgc gcgt
//
LOCUS       DASSNN010000139         6279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_155901, whole genome shotgun sequence.
ACCESSION   DASSNN010000139 DASSNN010000000
VERSION     DASSNN010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155901"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1165)
                     /locus_tag="VFL84_02470"
     CDS             complement(158..1165)
                     /locus_tag="VFL84_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Virginiamycin B lyase"
                     /protein_id="HET7607515.1"
                     /translation="MATFVTAGCASAQQRWLQSDERLAATGEVTELVLPHPMSGPTTI
                     ALAADGTVWFTESNGNRIGRMNPDGTGLVEFDVPTEDSSPRIIAFGSDGNAWFSEHLA
                     GQIARITPDGAIREFRIATPDSQPRAIALGADGNIWFGMFAAGKIGRVTPAGVITEFD
                     LPTPFSGPRALAAGPDGNIWFSEFHASKIGRITMDGVATEYPLPRPDSGPGDITAGAD
                     GNLWFVELNGTMDGRVVNGNRVGRITPRGEITEFQIPSPTGSPTNVAVGPDRNVWYTK
                     GASLGRVTPAGVITEFPLSANSRGVGLTAGSDRQPPERIANRLWYADGNGNRMGYLHF
                     E"
     gene            complement(1225..2448)
                     /locus_tag="VFL84_02475"
     CDS             complement(1225..2448)
                     /locus_tag="VFL84_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET7607516.1"
                     /translation="MTTRSLVSVAALLLCLTAVEAQRVDTRVELGARPALLVADLPDG
                     ELKSRLLACLDAPAKRTAFSIGHRGAGLVYPEHTLEAYEAAYRMGAGTIECDVTFTKD
                     LALVCRHAQNDLATTTDILLTPLASTCIAPFKPARLDANGRVRRPASAECRTSELTLA
                     EFKTLRGKIDAFDPAAQTVEQFVAPRRAARPDLGAGFMYGTLLTHAESIALFKRLGVR
                     MMPELKEASVRLPFNGITRDELSQKLIDEYRAAGVPPRDVYPQSFERRDVDYWIEHEP
                     EYGRQAILLDNVVLAPRAARLRRYKAAGINIWGPALPGVLALDDEGRIVPSRAARAAH
                     AAGLDIVTWTLERSGNLSAPNKGFYYRKIGPAVTREGDVLRVIDVLARDAGVRGVFSD
                     WPATVSFYAGCAGLP"
     gene            2573..3922
                     /locus_tag="VFL84_02480"
     CDS             2573..3922
                     /locus_tag="VFL84_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7607517.1"
                     /translation="MPSSHVLEPSRQTPVYGDYEVVVVGGGPAGIAAAIAAGRAGRST
                     ICIERYGFCGGAGTAAGLSTFCGLHAVIHGKHEQIVHGIADDILARLTRMDGLNKPHL
                     TIRDQILAQAYDISAFKIAADELLAEANVHVLFHAFATGAVMASDDRIEAVLVETKSG
                     RFAIRGRMFIDGSGDGDLAAWSGVPYEVGDGAGNMLYPSTMFRINGVDPQKAGRAWEL
                     VPKLMEEAEQRGRTFPRKKPIVRPQRNPIEWRANLTQIKNPNGTAVSGIDARELSYGE
                     VEGRKQCWDTFQFIKETTPGFDQAYIVEIAPQIGIRETRRVRGEYVLTEDDILGCRDF
                     ADTIGVQGWPVEAHIKGDVKFVFAPKDSRGFNEIPYRIIVPKKVNNLLVAGRCASMSH
                     EGQSSARVSGPCFIMGQAAGTAADLALQAGVAPRAIDVAALQRRLRADRANLGPSFG"
     gene            complement(3965..4978)
                     /locus_tag="VFL84_02485"
     CDS             complement(3965..4978)
                     /locus_tag="VFL84_02485"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HET7607518.1"
                     /translation="MTGAFCAACGQPAHIHRSFVSLGHDILHGAFHFDTKLWRTLPEL
                     VFRPGRLTRRYIDGERAKFISPMALYLLSVFLMYAVFSFTGGVAPTIGAEDIPGVSGF
                     NVDNSAAIEVTQQRLDELESQLAAPDVSAEQRAELEARRAGLQTSLQVMESIGRGDWA
                     NVERIRESVEDEIERERQAAQPADPNAAAADTEPTGRLERSLRDLNDNPGLVAYKLKT
                     NGYKFSWMLVPLSIPFMWPLFFWRRNVKAYDHAVFVTYSISFMMLLLIFVSLLGLVGL
                     GSGWIALILVVAVPLHIYKQLRGTYGCSRFGAFARVWILLLAALVVLSIYSVLLFFIG
                     ALD"
     gene            5228..6253
                     /locus_tag="VFL84_02490"
     CDS             5228..6253
                     /locus_tag="VFL84_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607519.1"
                     /translation="MSPRIPSQILMVAALALAGCSTPEDGASAASLTYPDGSPHPVTP
                     WGDPDLQGNWPIMHLFATRLQRDPKFGDRRELNDEEWQAAEAGLKRRDERYQDEIKTN
                     KMGMGHWAETTTRTAAARLTSLISYPPNGQLPALTARGEDLAPTFRSDDTQTVFDSVN
                     DFDAWDRCITRGLPASMLPFNYNNGIRIHQAPGYVVIDLEMIHEARVVPLDLPPLDPA
                     IKQWMGESRGHFEGATLVVETTNFTGKAPMLIAGIPGGVRGPRPSTVNMKTTERFTRV
                     SNDRIDYEMTIEDPEIQADKWTISYPMFLDPEYQFYEYACHEDNTAVRNFIATSQFER
                     GLRGKAK"
BASE COUNT         1074 a         2116 c         2128 g          961 t
ORIGIN      
        1 gggcgccggc gcgctggctt cctgcggggt gcaggcgacg agcgcgagcg aggcgacggc
       61 caggggtagg tagcgcatgg gggtctccgg tgccgatcgc cggcacgaaa acccggcacg
      121 ctagctgaat cggcgcgata aagccaatcg cgcccgctta ttcgaagtgc aggtagccca
      181 tccggttgcc gttgccatcc gcgtaccaga gccggttcgc gatgcgctcg ggtggctggc
      241 gatcgctgcc ggccgtcaag ccgacgccgc gcgaattggc cgacagtgga aattcggtga
      301 tgacgccggc cggcgtcacg cggccgagcg aggcgccttt cgtgtaccag acgttgcgat
      361 cgggcccgac ggcaacgttc gtcggcgagc ccgtcgggct cgggatctga aactcggtga
      421 tctcgccgcg cggcgtgatg cgcccgacgc gattgccgtt gacgacgcgc ccgtccatcg
      481 tgccgttcaa ctcgacgaac cagaggttgc cgtcggcgcc ggccgtgatg tcgccggggc
      541 cggagtccgg acgcggcagc gggtactcgg tcgcgactcc gtccatcgtg atgcgcccga
      601 tcttgctcgc gtgaaactcc gagaaccaga tgttgccgtc ggggccggcc gcgagcgcgc
      661 gcgggccgct gaacggagtc ggaaggtcga actcggtgat cacgccggcg ggcgtgacgc
      721 ggccgatctt gcccgcggcg aacatgccga accagatgtt gccgtccgcg ccgagcgcga
      781 ttgcgcgcgg ctggctgtcc ggagtcgcga tgcggaattc gcggatcgcg ccgtcgggcg
      841 tgatgcgcgc gatctggccc gccagatgct cggaaaacca cgcgttgccg tcggagccga
      901 acgcgatgat gcgcggcgag ctgtcttccg tcggcacgtc gaactcgacg agaccggtgc
      961 cgtcgggatt catgcggccg atgcggttgc cgttgctctc cgtgaaccac acggtcccgt
     1021 cggcggcgag cgcgatcgtc gtcgggccgc tcatcggatg cggcagcacg agctccgtga
     1081 cctcgccggt cgcggcgaga cgctcgtcgg actgaagcca acgttgctgc gccgaagcgc
     1141 acccggccgt gacgaacgta gccactacga acagcgctcc tcgcatgcgt accccgatga
     1201 cggcgatgcg tgatggtacc taagtcacgg caaacccgcg catcccgcgt agaagctgac
     1261 cgtcgcgggc caatcggaga aaacgccgcg cacgccggcg tcgcgcgcga gcacgtcgat
     1321 gacgcgcagc acgtcgcctt cgcgcgtgac cgcgggaccg atcttgcggt agtaaaagcc
     1381 tttgttcggc gccgatagat tgccggagcg ctcgagcgtc cacgtcacga tgtcgagccc
     1441 cgccgcgtgc gccgcgcgcg ccgcgcgcga aggcacgata cggccttcgt cgtcgagagc
     1501 gagaacgccg ggcagcgccg ggccccagat gttgatgccg gcggctttgt agcggcgcaa
     1561 gcgcgccgcc ctcggcgcga gcacgacgtt gtcgagcaag atcgcctgcc tgccgtattc
     1621 gggctcgtgc tcgatccagt aatcgacgtc gcgccgctcg aacgactgcg ggtacacgtc
     1681 gcgcggcggc acgcccgcgg cgcggtactc gtcgatcagc ttctggctga gctcgtcgcg
     1741 cgtgattccg ttgaacggca gccgcacgct cgcttccttg agctccggca tcatgcgcac
     1801 gccgagccgc ttgaacagcg cgatgctctc cgcatgcgtc agcaaggtgc cgtacatgaa
     1861 tcccgcgccg agatcgggac gagcagcgcg ccgcggcgcg acgaactgct cgaccgtctg
     1921 tgcggcggga tcgaacgcgt cgatcttgcc tcgcagcgtt ttgaattccg cgagcgtgag
     1981 ctcgctcgtg cggcattcgg cgctcgcggg gcggcgcacg cggccgttcg cgtcgagccg
     2041 cgcgggcttg aaaggcgcga tgcacgtgct cgcgagcggc gtcagcaaaa tatccgtcgt
     2101 cgtggcgagg tcgttctgcg catggcggca cacgagcgcg agatccttcg tgaacgtcac
     2161 gtcgcactcg atggtgccgg cccccatccg atacgcggct tcatacgcct cgagcgtgtg
     2221 ctcggggtag acgagccccg cgccgcggtg accgatcgag aacgcggtgc gcttcgcggg
     2281 cgcgtccaag caggccagca agcgcgactt cagctcgccg tcgggcaggt ccgccacgag
     2341 cagcgccggc cgcgccccga gctcgacgcg cgtgtcgacg cgctgcgctt ctaccgccgt
     2401 caggcagagc agcagcgcgg ctacgctcac gagcgatctc gtcgtcactt cggctccatg
     2461 ccgcgtttcg tccttatcga cgcccaagaa ccctttcgcg cgccgccccc cggcgtgact
     2521 cgcgcgggat tcctcggcac actgccctag aatcgcgcac cagagacatc gcatgccatc
     2581 gtcccacgta ctggagccca gccggcagac acccgtctac ggcgactatg aagtcgtcgt
     2641 cgtcggcggc gggccagccg gcatcgccgc ggcgatcgcc gcggggcgcg cgggacgatc
     2701 gacgatctgc atcgagcgct acggattttg cggcggcgcc ggcaccgcgg ccggcttgag
     2761 cacgttctgc gggctgcatg ccgtgatcca cggcaagcac gagcaaatcg ttcacggcat
     2821 tgccgacgac attctcgcgc ggctcacacg catggacggc ctgaacaagc cgcacttgac
     2881 gattcgcgac cagatcctcg cgcaggcgta cgacatctcg gccttcaaga ttgcggccga
     2941 cgagctgctc gcggaagcga acgttcacgt gctgttccac gcgttcgcga cgggcgccgt
     3001 catggcgagc gacgatcgca tcgaggccgt gctcgtcgag acgaagtcgg gccgcttcgc
     3061 aattcgcggc cgaatgttca tcgacggctc cggcgacggc gatctcgcgg cctggtccgg
     3121 cgtgccgtac gaggtcggcg acggcgccgg caacatgctg tacccgtcga cgatgttccg
     3181 catcaacggc gtggacccgc agaaggccgg gcgagcctgg gagctcgtgc cgaagctcat
     3241 ggaggaagcc gagcagcgcg gccgcacctt cccgcgcaag aagccgatcg tgcggccgca
     3301 gcgcaatccg atcgagtggc gcgcgaacct gacgcagatc aagaatccga acggcacggc
     3361 cgtcagcggc atcgacgcgc gcgagctctc ttacggcgag gtcgaaggcc gcaagcagtg
     3421 ctgggacacg ttccagttca tcaaggaaac gacgccgggg ttcgaccagg cctacatcgt
     3481 cgagatcgcg ccgcagatcg gcattcgcga gacgcggcgc gtgcgcggcg agtacgtgct
     3541 gaccgaggac gacatcctcg gctgccgcga cttcgccgat acgattggcg tgcaaggatg
     3601 gcccgtcgag gcgcacatca agggcgacgt gaagttcgtg ttcgcgccga aggactcacg
     3661 cggcttcaac gagattccgt accgcatcat cgtgccgaag aaggtgaaca atcttctcgt
     3721 cgcgggccgc tgcgcgtcga tgtcgcacga ggggcagtcg tccgcgcgcg tgtcgggtcc
     3781 ctgcttcatc atgggccagg cggccggcac ggccgccgat ctcgcgttgc aagccggcgt
     3841 cgcgccgcgc gccatcgacg tcgcggcgtt gcagcggcgc ttgcgcgccg accgagccaa
     3901 cctcggccct tccttcggtt gacctcgcgc cgccgcgagc tgcggccgct aaatgctcgg
     3961 ccgctcagtc gagagcgccg atgaagaaca acaagacgga gtagatcgac agcacgacca
     4021 gcgcggccag caagagaatc cacacgcgcg cgaaagcgcc gaaccgcgag cagccatagg
     4081 tgccgcgcaa ctgcttgtag atgtgcagcg gcaccgcgac cacgagaatc agcgcgatcc
     4141 atcccgagcc caagccgacg agaccgagca gcgagacgaa gatcagcagc agcatcatga
     4201 agctgatcga gtaggtcacg aacacggcgt ggtcgtaggc tttgacgttc cgccgccaaa
     4261 agaacagcgg ccacatgaac ggaatgctga gcggaacgag catccacgag aacttgtagc
     4321 cgttcgtctt gagcttgtac gcgacgagtc ccgggttgtc gttgaggtct cgcagcgagc
     4381 gctcgagccg gcccgtgggc tccgtgtccg ccgccgcggc gttcggatcg gccggttgcg
     4441 cggcttgccg ctcgcgctcg atctcgtcct cgacggattc ccgaattcgc tcgacgttgg
     4501 cccaatcgcc tcggccaatc gactccatga cttgcagaga ggtttggaga cccgcgcgcc
     4561 gagcctcgag ctcggcgcgc tgctcggcgg aaacgtcggg cgccgcaagc tgcgactcga
     4621 gctcgtcgag cctttgctgg gtcacctcga tggccgcgga gttgtcaacg ttgaagccgc
     4681 tgactcccgg gatgtcctcg gcgccgatgg tcggcgcgac gccgcccgtg aacgagaaca
     4741 ccgcgtacat caagaacacg ctgagcaaat agagcgccat cggcgagatg aacttcgcgc
     4801 gctcgccgtc gatgtagcgg cgcgtgaggc ggcctggccg gaacacgagc tcgggcagcg
     4861 tgcgccagag cttcgtgtcg aagtggaagg caccgtgcag gatgtcgtgg ccgagcgaga
     4921 cgaagctacg gtggatgtgc gccggttggc cgcaggccgc gcaaaaggca cccgtcaacg
     4981 tggccccaca attcaggcac gcggcgccgg cggcctccga gccgccccga gccccgcttc
     5041 cggcgctcgg ctcgacagcg ttggagatga gcgcgcccgt cacggcgtct gctgctgctt
     5101 cggtagggtc cgtcacggcg aaagaggata gccgcgcccc cgcgtcgatg ataggcgggc
     5161 cccgcgggcc gcgcctataa ttgccgcttc cgcggcgccg agccgcggcc cgagtcggag
     5221 acaggcgatg agcccacgca taccctcgca gattctgatg gtagccgcgc tcgctttggc
     5281 gggctgctcg acgcccgagg acggcgcgtc ggcggcatcc ttgacgtatc ccgacggctc
     5341 gccgcatccc gtgacgccgt ggggcgatcc cgaccttcaa ggcaactggc cgatcatgca
     5401 tctgttcgcg acgcgcctgc agcgcgatcc gaaattcggc gaccgccgcg agctcaacga
     5461 cgaggagtgg caggccgccg aggccggtct caagcgccgc gacgagcgct atcaagacga
     5521 gatcaagacg aacaagatgg gcatgggcca ctgggccgag acgacgacgc gcacggcagc
     5581 ggcccggctc acgtcgctga tttcgtatcc gccgaacgga caattgccgg cgctgaccgc
     5641 gcgcggcgaa gacctcgcgc cgacgttccg cagcgacgac acgcagaccg tgttcgactc
     5701 cgtcaacgac ttcgatgcgt gggaccgctg catcacacgc ggcctgccgg cgtcgatgct
     5761 gccgttcaac tacaacaacg gcattcgcat tcaccaagct cccggctatg tcgtcatcga
     5821 cttggagatg atccacgagg cgcgggtcgt gccgctcgac ttgccgcctc tcgatcccgc
     5881 gatcaagcaa tggatgggcg agtctcgcgg ccacttcgaa ggcgcgacgc tcgtcgtcga
     5941 gacgacgaac ttcacgggca aggcgccgat gctgatcgcc gggatcccgg gcggcgtgcg
     6001 cgggccgcga ccgtcgacgg tgaacatgaa gacgaccgag cggttcacgc gcgtcagcaa
     6061 cgatcgcatc gactacgaga tgacgatcga ggatcccgag atccaagccg acaagtggac
     6121 gatcagctac ccgatgttcc tcgatcccga gtaccagttc tacgagtacg cgtgccacga
     6181 ggacaacacg gccgtgcgca acttcatcgc cacgtcgcag ttcgagcgcg gcttgcgcgg
     6241 aaaagcgaag tagcagttag ggtttctgcg ccgcgatct
//
LOCUS       DASSNN010000140         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1274699, whole genome shotgun sequence.
ACCESSION   DASSNN010000140 DASSNN010000000
VERSION     DASSNN010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274699"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFL84_02495"
     CDS             complement(<1..402)
                     /locus_tag="VFL84_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 family protein"
                     /protein_id="HET7607520.1"
                     /translation="MTYLPLLGIALVVLGFALRFNPLLVVAIAAVATGLLAGLPFVKV
                     LATLGKAFNDNRYVSIIWIVLPVIGVLERYGLQQQARTLIGRMRGATTGRILLTYLGF
                     RQILAALGLTSVAGHPQTVRPLVAPMAEAAAE"
     gene            complement(399..>2038)
                     /locus_tag="VFL84_02500"
     CDS             complement(399..>2038)
                     /locus_tag="VFL84_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET7607521.1"
                     /translation="PGWRAERVDDCTLVLTRTVPLQRAKAIGTDVDPVQLEIFNNLFM
                     AIAEEMGVALQATATSVNIKERLDFSCAIFDSQGALIANAPHIPVHLGSMGESIRRII
                     DTRGQCRDGRGILRGDAYVLNDPYRGGTHLPDITVIVPVFTGDEAEPSAFVAARGHHA
                     DVGGIAPGSMPPDSRTIDEEGVLIDNELLVGEGHFREAEIRALLGSGGWPARNPDRNI
                     SDLKAQLAACTRGAEVLAQTARDYGREVVAAYMRHVLANAEESVRRLLGRLDDGTFDY
                     EMDNGGQVRVRITVDKQARSAVFDFTGTSGQLADNFNAPFSIVRAASLYVVRTLIDDA
                     IPMNDGCLRPIELVVPDGSMLNPKYPAAVVAGNVETSQVVTDSLFAATGRLAPSQGTM
                     NNFTFGNAAHQYYETIAGGSGAGPDHDGTSAVQTHMTNSRLTDPEILETRLPVRLEQF
                     AIRRGSGGAGAHRGGDGVVRAVTFLEPMRANILANRRRVAPRGIAGGADAEAGRNWVE
                     RTDGSIEMLSATASAEMQPGDRFVIETPGGGGYGAAR"
BASE COUNT          327 a          674 c          696 g          341 t
ORIGIN      
        1 ttcggccgcg gcttcggcca tcggcgcgac cagcggccgc acggtctgcg ggtgacccgc
       61 gacagaggtc aggccgagcg ccgcgaggat ttgccggaag ccgagatagg tgaggagaat
      121 tcggccggtc gtcgcgccgc gcatccggcc gatcagagtc cgcgcctgct gctggagacc
      181 gtagcgttcg agcacgccga tgaccggaag gacgatccag atgatcgaga catatcggtt
      241 gtcgttgaac gccttgccga gcgtcgcgag caccttgacg aacggcaatc cggcaagaag
      301 gccggtcgcg acggcggcga tcgcaaccac gagcaaggga ttgaagcgaa gcgcgaagcc
      361 gaggacgacg agggcgattc caaggagcgg gagataggtc atcgcgcggc tccatagccg
      421 ccgccgccgg gagtctcgat gacgaagcgg tcgccgggct gcatctcggc ggacgcggtg
      481 gcgctcagca tctcgatgct gccgtcggtg cgctcgaccc agttgcggcc cgcttccgca
      541 tccgcaccgc ccgcgatgcc gcgcggagca acccggcggc gattggcgag aatgttggcg
      601 cgcatcggct cgaggaaggt gacggcgcgg accacgccgt cgccgcccct gtgcgcccct
      661 gccccgcccg agccgcgccg gatcgcgaac tgttcgagtc tgactggaag ccgggtttcg
      721 aggatttccg ggtcggtcag gcggctgttc gtcatgtgcg tctgcacggc gctcgtcccg
      781 tcatgatccg ggccggcccc cgagccgccg gcgatcgtct cataatattg gtgcgccgcg
      841 ttgccgaagg tgaaattgtt catcgtcccc tggctcggcg caagtcggcc cgttgcggcg
      901 aatagcgagt cggtaacgac ctggctggtc tcgacattgc ccgcgaccac tgccgccggg
      961 tacttcgggt tgagcatcga cccgtccggc accaccagct cgatcggccg gaggcagccg
     1021 tcgttcatcg gaatcgcgtc gtcgatcagc gtccggacga cgtacagcga tgccgcgcgg
     1081 acgatcgaga agggcgcgtt gaaattgtcg gcgagttggc cgctcgtgcc ggtgaaatcg
     1141 aacaccgccg agcgcgcctg cttgtcgacc gtgatccgca cgcggacttg gccgccgttg
     1201 tccatttcgt aatcgaacgt gccgtcgtcg agccggccga gcagccgccg caccgactcc
     1261 tcggcattgg cgagcacgtg ccgcatgtag gccgcaacga cctcacgccc gtaatcgcgc
     1321 gccgtctgcg cgagcacctc cgcgccgcgc gtgcaggccg cgagctgcgc cttcagatcg
     1381 gagatgttgc gatcgggatt gcgcgccggc catccgcccg aaccgagcag cgcccgaatc
     1441 tcggcctcgc ggaaatggcc ttcgccgacg agcaactcgt tgtcgatgag cacgccttct
     1501 tcatcgatcg tgcgactgtc cggcggcatc gatccgggcg cgatcccgcc gacgtccgca
     1561 tggtggccgc gcgctgcgac gaatgccgac ggttccgctt cgtcgccggt gaagaccggc
     1621 acgatcacgg tgatgtcggg cagatgcgtg ccgccgcgat acggatcgtt cagcacgtaa
     1681 gcatcgccgc gcaggatgcc gcggccgtcc ctgcactgcc cgcgtgtgtc gatgatcctg
     1741 cgaatgctct cgcccattga gcccaggtgc accgggatgt gcggcgcgtt ggcgatcagc
     1801 gcgccttgcg aatcgaagat cgcgcaggag aaatcgagcc gctccttgat gttgacgctc
     1861 gtcgccgtcg cctggagcgc gacgcccatt tcctcggcga tcgccatgaa caggttgttg
     1921 aagatttcga gctgcaccgg gtcgacatcg gtgccgatcg ccttcgcgcg ttggagagga
     1981 acggtgcgtg tgaggaccag cgtgcaatca tctacgcgtt cggctcgcca gccgggtt
//
LOCUS       DASSNN010000141         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1274861, whole genome shotgun sequence.
ACCESSION   DASSNN010000141 DASSNN010000000
VERSION     DASSNN010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274861"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="VFL84_02505"
     CDS             <1..1286
                     /locus_tag="VFL84_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7607522.1"
                     /translation="RYAARESLKIVPRGAGTSLSGGAIPAADAIVLGLGKLNRILEID
                     YANRCVVAQSGVTNLAVSRAVAERGFYYAPDPSSQVACTLGGNVAENSGGVHCLKYGL
                     TTNNVLGVELVTPEGDILRLGGRHLDSGHYDLLGLTVGSEGLIGVVTEVTVRILKRPQ
                     ASRALLIGFPVLEDAGACVAAVIAAGIVPAGMELMDRPAIHAAEDFVAAGYPLDVEAL
                     LIVELDGAEAEVEALAERVGALAKEHGAASCRVSSDERERLAFWAGRKAAFPAVGRIS
                     PDYYCMDGTIPRRELARVLRAMSEMSERYGLRVANVFHAGDGNLHPLILYDANVPGEL
                     ERAEAFGADILKLCVDAGGVLTGEHGVGLEKRDLMSYQFDEADLEVQHRIKRAFDPAL
                     RFNPGKVFPTLHRCAELGREHVHAGAERFPDLPRF"
     gene            1381..>2157
                     /locus_tag="VFL84_02510"
     CDS             1381..>2157
                     /locus_tag="VFL84_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7607523.1"
                     /translation="MKFDETADAQQTARRGVLRPRDAADVAEILRTHAGAVEPLGGAS
                     KRSLGGPVEADVLDLAALSGILDYEPAELVLTARAATPVAAVEALLAEHGQRLAFEPP
                     SFGAMLGVAASETLGGVLAANLSGSRRLTAGGARDHFLGFKAVTGDGLPFKGGGRVVK
                     NVTGYDLPKLLAGSWGTLAVLTEVSLRVAPAAETESTLIVPLGSARADRVRTAVAMLT
                     QAVSSYNEVSCAAFDPSRGVALRIEGFGSSVQARARGLLEL"
BASE COUNT          318 a          723 c          760 g          356 t
ORIGIN      
        1 tgcgttacgc cgcccgcgaa agtctcaaga tcgttccgcg cggcgcggga acctcgctct
       61 cgggcggagc gatcccggcg gccgacgcca tcgtgctcgg tctcggcaag ctcaatcgca
      121 tcctagagat cgactacgcg aatcgctgcg tcgtcgcgca aagcggggtc acgaatctcg
      181 ccgtgagccg cgcggtcgcg gagcgcggct tttactatgc gcccgatcct tcgagccagg
      241 tcgcctgcac gctcggcggc aacgtcgcgg aaaattcggg cggagtgcac tgcctcaagt
      301 acggacttac caccaacaac gtgctcggcg tcgagctcgt gacgcccgaa ggggacatcc
      361 tgcggctcgg cggccggcat ctcgatagcg ggcattacga tctgctcggc ctgacggtcg
      421 gttccgaagg gctcatcggc gtggtcaccg aagtcacggt gcggatactc aagcgcccgc
      481 aggcctcgcg cgcactgctc atcggctttc cggtcctcga ggacgccggg gcctgcgtcg
      541 cggccgtcat cgccgccggc atcgttcccg ccggcatgga gctcatggat cgtccggcga
      601 ttcacgccgc cgaggacttc gtggccgcag gttatccgct cgacgtcgag gcgctgctca
      661 tcgtcgagct cgacggcgcc gaggcggagg tcgaggccct cgccgagcga gtcggggcgc
      721 tcgcgaagga gcacggcgcc gcgagctgcc gcgtttcctc ggacgagcgc gaacggctcg
      781 cattttgggc ggggcgcaag gctgcgtttc cggccgtcgg ccgtatcagt ccagactact
      841 attgcatgga tggaactatc ccgcgccgcg agctcgcgcg cgtgctgcgc gccatgagcg
      901 agatgtccga gcgctacggc ttgcgcgtcg cgaatgtctt tcacgcgggc gacggcaatc
      961 tgcatccgtt gattctgtac gatgccaacg tgccgggcga gctcgaacgc gccgaagcct
     1021 tcggcgcaga catcctgaag ctttgcgtgg acgcaggcgg cgtgctcacg ggagagcatg
     1081 gtgtcggcct cgagaagcgc gatctcatga gttaccagtt cgacgaggcg gacctcgaag
     1141 tgcagcaccg catcaagcgc gcgttcgacc ccgcgctgcg cttcaatccc ggcaaggtgt
     1201 ttcctacgct gcatcgctgc gccgagctcg gccgcgaaca tgtgcatgcg ggcgccgagc
     1261 gctttccgga cttgccgcgg ttttgatagg ggacgtgatg cgagccggga tgaaacgaga
     1321 acgcgagccg agccgaatcg aggagcagct gccgatgacg acgctcgcgg atgcgagggc
     1381 atgaaattcg acgagacggc ggatgcacag cagacggcgc gccgaggcgt gcttcggccg
     1441 cgcgacgccg ccgacgtagc cgaaattctg cgcacacatg ccggcgcggt cgagccgctc
     1501 ggcggcgcct cgaagcgctc gctgggcggt cccgtcgagg ccgatgtgct cgatctcgcc
     1561 gcgctctccg gcatcctcga ttacgagccg gccgagctcg tgcttacggc gcgcgccgcg
     1621 acgccggtcg ccgcggtcga agccctgctg gccgagcacg ggcagcggct cgcgttcgag
     1681 ccgccgtctt tcggcgcgat gctcggtgtc gccgcgtccg agacgctggg cggagtgctc
     1741 gcggccaatc tgtcggggtc gcgccggctc acggccggcg gcgcccgcga tcacttcctc
     1801 ggcttcaagg cagtgacggg cgacggcctg ccgttcaagg gcggcggccg ggtcgtcaag
     1861 aacgtcaccg gctacgactt gccgaagctg ctcgccggct cctggggcac gttggcggtg
     1921 ctgaccgagg tgagcctacg ggtcgctccg gcggccgaga cggagtcgac gctcatcgtg
     1981 ccgctcggtt cggcccgcgc cgaccgcgtg cggaccgccg tcgcgatgct gacgcaggcg
     2041 gtatcgtcgt acaacgaggt gtcttgcgcg gccttcgatc cgagccgcgg cgtcgcgctc
     2101 agaatcgagg gattcggttc atcggtgcag gcgcgcgctc gcggtctcct cgagctg
//
LOCUS       DASSNN010000142         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_644947, whole genome shotgun sequence.
ACCESSION   DASSNN010000142 DASSNN010000000
VERSION     DASSNN010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644947"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1480
                     /locus_tag="VFL84_02515"
     CDS             74..1480
                     /locus_tag="VFL84_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007820812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HET7607524.1"
                     /translation="MAAHNLSAVLKEHGNAVKLLRNSKIGMYVYPVVAPEFSNWRNEQ
                     VAWRKSAVLFDQTHHMDELIVEGPDSAAFLEYVGINSFANFDLNRAKHFVPVTPAGHV
                     IGDMIIFREREDKFVLVGRAPTANWVRFQQAVGKWKVRLTHDPRSDSRPDGKAIYRTH
                     YRFQIQGPESNKIFDKINGGPVPDIKFFHVDWIKIGTRRVQALRHGMAGAPGLEVWGP
                     YADKHYIQSTILQAARDVGVDLHLVGSRAYSTNTLESGWIPSPLPGIYTGGGMLQEYR
                     DWLGADSYEAVGSIGGSLATDNIEDYYVNPFELGYGFYIGWKTDFIGKDALTKMKDQK
                     NRKKVTLEWNRDDLLKVVASSFEEGVPYKWIDFPQPNYASTNADLLLTAKKQAGISMF
                     NGYSFNERCMLSLAVVDDDVNIGDQLTLIWGEPEKTGKTSTEPHRQAEIRVRVSPTPY
                     SREARENYADSWRTKTGK"
     gene            1501..2874
                     /locus_tag="VFL84_02520"
     CDS             1501..2874
                     /locus_tag="VFL84_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HET7607525.1"
                     /translation="MTYENLEQALNAAKSPVDMLRNSQIGPYAFPVVPNEFTNWRDEQ
                     RSWRETCALFDQSHHMTDLYLEGPDALKLLSALGINTFNNFRVDTAKQFVACNHDGYV
                     IGDAILFHLAPNSFVLVGRPPALNWVQYNLEAGHYNAKAERDERSFVNKTGGRRSYRY
                     QVQGPNAKKVMEKVTGKPVPEIKFFNMDRFQIAGCTVRALRHGMVGQPGWELFGPWAD
                     ADKVRDAIVEAGRDLGIRKVGARTYPTSTLESGWIPSPVPAVYTGELMKPYREWLKGT
                     SYEATASLGGSFVSKNIESYYLTPYDLGYGGFVRFDHEFIGRAALEKMAKKPKRQKVT
                     LVWNGDDVDKVFSKLFHAGKFTKYIDLPLANYATLPFDKIVKGGKTVGVSTYTGYSFN
                     ERAMLSLACIDVKHAKPGTKVSVVWGEDGGGTKKPTVERHQQATIRATVQPAPYGETA
                     RTAYRPR"
     gene            complement(2929..>3385)
                     /locus_tag="VFL84_02525"
     CDS             complement(2929..>3385)
                     /locus_tag="VFL84_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607526.1"
                     /translation="WMRALAWAEGVRIGMASVSAGLYSAAFADAAGDAAAGSVSREMA
                     TELSRGFGALSSGYQNLSGEYVRFARRRYEASAQSRDFFFMMIQHEDRRVALAQVSKR
                     DGRVLSEIDLGRDKEPAYQVDDVSSSVFYRPADSVVAGYRFAPQRVALQ"
BASE COUNT          650 a         1118 c         1091 g          526 t
ORIGIN      
        1 gctccgttcg cgaaccggga ggcgagcggt ccgaagacgc tcgcctcttt tttcattgac
       61 gacacgagga aatatggccg cacacaatct gagcgccgtg ctgaaggagc atggcaacgc
      121 cgtcaagctt ctgcgcaact cgaaaatcgg catgtacgtc tacccggtcg tcgcgcccga
      181 gttctcgaac tggcgcaacg agcaggtcgc ctggcgcaag tcggccgtgc tgttcgacca
      241 gacccatcac atggacgagc tcatcgtcga gggtccggac tcggccgcat tcctcgagta
      301 cgtcggcatc aacagcttcg cgaacttcga tctgaaccgc gcgaagcact tcgtgccggt
      361 cacgccggcc ggccacgtca tcggcgacat gatcatcttc cgcgagcgcg aggataagtt
      421 cgtcctcgtc ggccgcgccc cgacggcgaa ttgggtgcgg ttccagcagg ccgtgggcaa
      481 gtggaaggtg cgcctcacgc acgatccgcg ctccgacagc cgccccgacg gcaaggcgat
      541 ctatcgcacg cactaccgct tccagatcca ggggcccgag tcgaacaaga tcttcgacaa
      601 gatcaacggc ggccctgtcc cggatatcaa gttcttccac gtcgactgga tcaagatcgg
      661 aacgcgccgc gttcaggcgc tgcgccacgg catggccggt gcgccgggcc tcgaggtctg
      721 gggcccgtac gccgacaagc actacattca gagcacgatc ctgcaggccg cgcgcgacgt
      781 cggcgtcgac ctgcatctcg tcggcagccg cgcctactcg acgaacacgc tcgagtcggg
      841 ctggatcccc tcgcctttgc ccggcatcta taccggcggc ggcatgctgc aggaataccg
      901 cgactggctc ggcgccgata gctacgaggc tgtcggctcg atcggcggca gcttggccac
      961 cgacaacatc gaggactact acgtcaatcc gttcgagctc ggctacggct tctacatcgg
     1021 ctggaaaacc gacttcatcg gcaaggacgc gctgaccaag atgaaggacc agaagaaccg
     1081 caagaaggtc acgctcgagt ggaaccgcga cgatctgctc aaggtcgtcg cgtcgtcgtt
     1141 cgaggaaggc gtgccctaca agtggatcga cttcccgcag ccgaactacg cgtcgacgaa
     1201 tgctgacctc ttgcttacgg ccaagaagca ggccggcatc tcgatgttca acggctactc
     1261 gttcaacgag cgctgcatgc tgtcgctcgc ggtcgtcgac gacgacgtga acatcggcga
     1321 tcagctcacc ttgatctggg gcgagcccga gaagacgggc aagacctcga cggagccgca
     1381 ccggcaggcc gagatccgcg ttcgcgtctc gccgacgccg tattcgcgcg aagctcgcga
     1441 gaattacgcc gacagctggc gcacgaagac gggaaagtga gcggagccgg ataagccttt
     1501 atgacctacg agaatctaga acaggcgttg aacgccgcga agagccctgt cgacatgctg
     1561 cgcaactcgc agatcgggcc ttacgctttt cccgtcgtgc cgaacgagtt cacgaactgg
     1621 cgcgacgagc agcgctcgtg gcgcgagacc tgcgcgctgt tcgaccagtc gcatcacatg
     1681 acggacctct acctggaagg tcccgatgcg ctgaagctgc tttccgctct cggcatcaac
     1741 acgttcaaca actttcgcgt cgacaccgcg aagcagttcg tcgcctgcaa ccacgacggc
     1801 tacgtgatcg gcgacgcgat cctgttccac ctcgcgccga atagcttcgt gctcgtcggc
     1861 aggccgccgg cgctgaactg ggtgcagtac aacctcgagg ccgggcacta caacgcgaaa
     1921 gccgagcgag acgagcgctc gttcgtcaac aagaccggcg ggcggcggtc gtatcgctat
     1981 caggtacagg gtccgaacgc gaagaaggtc atggagaagg tcacgggcaa gcccgtgccc
     2041 gagatcaagt tcttcaacat ggaccgcttc cagatcgccg gctgcaccgt gcgcgcgctg
     2101 cgccacggca tggtcggcca gcccggctgg gagctgttcg gcccgtgggc cgacgccgac
     2161 aaggttcgcg acgcgatcgt cgaagccggg cgcgacctcg gcattcgtaa ggtcggcgcg
     2221 cgcacgtatc cgacgagcac gctcgagtcg ggctggatcc cgtcgcccgt cccggccgtc
     2281 tacacgggcg agctcatgaa gccgtatcgc gagtggctga aaggcacgag ctacgaggcg
     2341 acggcctcgc tcggcggcag cttcgtctcg aagaacatcg agagctacta cctgacgccg
     2401 tacgacttgg gctacggcgg cttcgtgcgg ttcgaccacg agttcatcgg ccgcgccgcg
     2461 ctcgagaaga tggcgaagaa gccgaagcgg cagaaggtca cgctggtctg gaacggcgac
     2521 gacgtcgaca aggtgttcag caagctgttc cacgccggca agttcacgaa gtacatcgac
     2581 ctgccgctcg cgaactacgc gacgctgccg ttcgacaaga tcgtcaaggg cggcaagacc
     2641 gtcggcgtgt cgacgtacac cggctacagc ttcaacgagc gcgcgatgct ctcgctcgcg
     2701 tgcatcgacg tgaagcacgc gaagccgggc acgaaggtct cggtcgtctg gggcgaggac
     2761 ggcggcggca cgaagaagcc gaccgtagag cgtcaccagc aggcgacgat tcgcgcgacc
     2821 gtgcagcccg cgccgtacgg cgagactgcg cgcacggcgt accggccgcg ctaacggcgt
     2881 gggcgtgggc gtgggcgtgg gcgtgcgccc acgccgtccg gcgccgcgct actggagcgc
     2941 gacgcgctgc ggcgcgaagc ggtagcccgc gacgaccgag tcggcgggcc ggtagaacac
     3001 ggagctcgag acgtcgtcga cttgataggc cggctctttg tcgcgcccga gatcgatctc
     3061 gctcaagact cgcccgtcgc gcttgctgac ctgcgcgagc gcgacgcggc gatcctcgtg
     3121 ctggatcatc atgaagaaga agtcacgcga ctgtgccgaa gcctcgtagc gccggcgcgc
     3181 gaagcgcacg tactcgcccg acaggttttg atagccggac gataaggcgc cgaacccgcg
     3241 cgagagctcc gtcgccatct cgcggctgac ggacccggcg gccgcgtcgc cggcggcgtc
     3301 cgcgaacgcg gcgctgtaga gaccggccga cacggaagcc attccgatgc gcacgccctc
     3361 ggcccacgcc agcgcgcgca tccag
//
LOCUS       DASSNN010000143         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1541693, whole genome shotgun sequence.
ACCESSION   DASSNN010000143 DASSNN010000000
VERSION     DASSNN010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541693"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VFL84_02530"
     CDS             <1..812
                     /locus_tag="VFL84_02530"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET7607527.1"
                     /translation="RVNFGSMTKPLHVGRAGFNGVTAAELAARGFTGGKDALDPPWGF
                     FATFGHGAEHDPARIVGKLGDPHTIVSPGVSIKPYPCGVLGHPTMDAMRRLVTKHDVR
                     PEQIAAIRVRAGSNILNPLRYPIATNELEAKFCPAFMVSAIALRRKAGIREFNDEFVR
                     SAPVQALQRRVERVLDPEIEAKGFEKIRSTVEVDLVDGRKLVEHADERYRGGPDLPFT
                     RDELYEKWSDCASLVLPKAGVDEVFELVDGLERMSDITTLVRALTASAKKR"
     gene            824..2083
                     /locus_tag="VFL84_02535"
     CDS             824..2083
                     /locus_tag="VFL84_02535"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET7607528.1"
                     /translation="MSLAWISVAALVLAITLSCTTTINVGVLSLALALVVGVGLAGMD
                     AGAVLDGFPTDLFITLLGVTLLFSIAEVNGTLARLTERAVRLCRGHAGALPVMFFVIG
                     FVVATIGAGATPASALLAPPAMAVAARAGVPPLLMAIMAGNGALAGTLSPFAPTGVVA
                     HSNMEQIGLAGYEWQTFTYNALAHALVGIGGFLLLGGWKLFGRGARRADAADVETVEA
                     MSAKHWLTTLGIVALIASVVGFDAAGFDVDVGMVAVIVATVLVLLRTVDESKAIARMP
                     WGVIVMVTGVTVLIALLRETEGLKLITTGIANLSTPATIEPIVAFGVGLVSVYSSTSG
                     VVLPAFLPMVPDLAMRLGGLDPLPIAWSMNVAASLVDLSSLSTGGALFIAGAAGFADT
                     RKLFNQLLAWGMSMTLVGAALCYVLFG"
     gene            complement(2199..>2544)
                     /locus_tag="VFL84_02540"
     CDS             complement(2199..>2544)
                     /locus_tag="VFL84_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607529.1"
                     /translation="VLNLYVIDDESTAVLQRLGLEFPGTHQIAIFIAMTFVSGLIMIF
                     LYAAMRPRFGAGAKTAVIAGLVVWFLGMMAAFGDVVLGITPANLLVIAGAWSLVETIV
                     AAIAGAWVYKEA"
BASE COUNT          392 a          848 c          877 g          427 t
ORIGIN      
        1 tccgcgtgaa tttcggcagc atgacgaagc cgctgcatgt cggccgcgcg ggcttcaacg
       61 gcgtgacagc cgcggagctc gcggcgcgcg gcttcacggg cggcaaggac gcgctcgatc
      121 cgccgtgggg atttttcgcg acgttcggcc acggcgcaga gcacgacccg gcgcgcatcg
      181 tgggcaagct cggcgacccg cacacgatcg tctcgcccgg cgtctcgatc aagccgtacc
      241 cgtgcggcgt gctcggccac ccaacgatgg atgcgatgcg tcgcttggtc acgaagcacg
      301 acgtgaggcc cgagcaaatc gccgcgatcc gcgtgcgcgc gggctcgaac atcctgaacc
      361 cgctgcgcta tccgatcgcg acgaacgagc tcgaggcgaa gttctgcccc gcgttcatgg
      421 tcagcgcgat cgcgctgcgg cgcaaagcgg gcattcgcga gttcaacgac gagttcgtgc
      481 ggagcgcgcc cgtgcaagcg ctgcagcgcc gtgtcgagcg cgtcctcgat cccgagatcg
      541 aggcgaaggg gttcgagaag atccggagca cggtcgaggt cgatctcgtc gacggccgca
      601 agctcgtcga gcacgccgac gagcgttacc gcggcgggcc agacttgccg ttcacgcgcg
      661 acgagctgta cgaaaagtgg agcgactgcg cgtcgctcgt gctgccgaag gcgggcgtgg
      721 acgaggtgtt cgagctcgtc gacgggctcg agcgcatgag cgacatcacg acgctcgtgc
      781 gtgcgctgac ggcgtcggcc aagaagcgct aggccgactc gcgatgagcc tcgcgtggat
      841 ctccgtcgcg gcgctcgtct tggccatcac gctctcctgc acgacgacga tcaacgtcgg
      901 cgtgctgtcg ctcgcactcg cgctcgtcgt cggcgtcggc ctcgcgggca tggacgcggg
      961 cgccgtgctc gacggctttc ccacggatct cttcatcacg ctgctcggcg tgacgctgtt
     1021 gttctcgatc gccgaggtca acggcacgct cgcgcgcttg acggagcgcg ccgtgcggct
     1081 ctgccgcggg cacgcaggcg cgctgcccgt gatgttcttc gtcatcgggt tcgtcgtcgc
     1141 gacgatcggc gcgggcgcga cgccggcgag cgcgctgctc gcgccgcccg cgatggccgt
     1201 cgccgcgcgc gccggcgtgc cgccgctcct gatggcgatc atggccggca acggcgcgct
     1261 ggccggcacg ctgtcgccgt tcgcgccgac gggcgtcgtc gcgcacagca atatggagca
     1321 gatcggcctc gccggttacg agtggcagac gttcacgtac aacgcgctcg cgcacgcgct
     1381 cgtcggcatc ggcgggttct tgctgctcgg cggctggaag ctgttcgggc gcggcgcgcg
     1441 gcgcgccgat gcggccgacg tcgaaaccgt cgaggcgatg agcgcgaagc attggctgac
     1501 gaccttaggg atcgtcgccc tgatcgcgag cgtcgtcggc ttcgacgcgg cggggttcga
     1561 cgtcgacgtc ggcatggtgg ccgtcatcgt cgcgaccgtg ctcgtgctgc tccgcaccgt
     1621 cgacgagtcg aaggccatcg cgcgcatgcc gtggggcgtg atcgtcatgg tgacgggcgt
     1681 caccgtgctg atcgcgctgc tgcgcgagac ggagggcctt aagctgatca cgacgggcat
     1741 cgcgaacctg tcgacgccgg cgacgatcga gccgatcgtc gcgttcggcg tcgggctcgt
     1801 atccgtctac agcagcacgt cgggcgtcgt gttgccggcg ttcctgccga tggtgcccga
     1861 tctcgcgatg cggctcggcg gcctcgaccc gctgccgatc gcgtggtcga tgaacgtcgc
     1921 cgcgagcctc gtcgacctct cgtcgctgtc gacgggcggc gcgctgttca tcgcgggcgc
     1981 cgcgggcttc gccgacacgc gcaagctgtt caaccagctc ctcgcgtggg gcatgtcgat
     2041 gacgctcgtc ggcgccgcgc tctgctacgt tctattcggc taagagcggc tgaaagggga
     2101 cagatctatt ttccgcgcat gaagagattt gcttccgtag caacgtacgt cggcgcggaa
     2161 aatagatctg tccccgttta gcccgagccg cgcagtcctc aggcctcttt gtagacccaa
     2221 gcgccggcaa tcgctgcgac aatcgtctcg acgagactcc atgcgccggc gatcacgagc
     2281 agattcgccg gtgtgatgcc gagaaccacg tccccgaaag ccgccatcat gccgagaaac
     2341 cagacgacga gacctgcaat gaccgctgtc ttcgcgccgg cgccgaagcg cggccgcatt
     2401 gcagcgtaca agaagatcat gatgaggccc gacacgaacg tcatcgcgat gaagatcgcg
     2461 atttgatgcg tccccggaaa ctccaagccg aggcgctgca aaactgccgt ggactcgtcg
     2521 tcgatgacgt acaggttcag aacg
//
LOCUS       DASSNN010000144         3210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1541789, whole genome shotgun sequence.
ACCESSION   DASSNN010000144 DASSNN010000000
VERSION     DASSNN010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541789"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1837
                     /gene="asnB"
                     /locus_tag="VFL84_02545"
     CDS             95..1837
                     /gene="asnB"
                     /locus_tag="VFL84_02545"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET7607530.1"
                     /translation="MCGFIGGTDPDWSYAAALASIAHRGPDAALLSLDGPVKVGFRRL
                     SIIDLRPSANQPMLAPDGASWIVFNGEIYGYRELRSTLQKRGRVFLTDSDTEVALHAY
                     LEWGADFVDRIDGMFAIVIWDARDRKLRLYRDRPGIKPLYYYYDGSRFAFASELKALE
                     AALRPGELAIDGTACYDFLGYRYVPAPKTLYKRCFKLEPAHELVYAPDTGSLSAPRRY
                     WSLPIPAEPRAPAVEAACEELRSLVAASVVEQMIADVPLGFFLSGGVDSSVVVAAAAA
                     TGTRVSTFSIGFDSDEVSETPFAREVARRFATDHHERILSQAHAQELLPRLKAWFDEP
                     FADESSMPTYLVCAAAREHVTVVLTGDGGDEVFGGYRTYPRYARYARWPTWPAAMERA
                     AYALRRPFPRRHAVARFVTLLETALSSGPNLWAKLMGGMSTPAKRAYASELGIPADYD
                     DWWHYREHWRDDLPLRTRLQYVDFHTFMPGLVLTKVDRTSMAVSLEARVPLLARRVIE
                     FSFGLDEDLRYYGGEPKGLLRQAYRGILPDHILDRRKKGFGIPRYYLKDVGGGRLIQE
                     HVLRSLFLGATVEN"
     gene            1827..2930
                     /gene="gmd"
                     /locus_tag="VFL84_02550"
     CDS             1827..2930
                     /gene="gmd"
                     /locus_tag="VFL84_02550"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET7607531.1"
                     /translation="MKTEGKGKKALITGITGQDGAYLAEFLLAKGYEVHGIKRRASSF
                     NTDRIDHLYRDPHEPDRKMILHYGDLTDATNLIRVVQEVRPQEIYNLAAQSHVAVSFE
                     TAEYTANADALGTLRLLEAIRICELGAKTRFYQASSSEMYGKVVETPQRETTPFYPRS
                     PYAAAKVYAYWITVNYREAYGLYACNGILFNHESATRGETFVTRKITRGLARIKAGLD
                     DCLYLGNLDALRDWGHARDYVRAQWLMLQQDKPEDYVIATGEQHSVREFVERAAQALG
                     MQLRWHGSKLDEHAVEASSGRTVVRIDPRYFRPTEVDTLLGDASKARKQLGWVPEIGF
                     DQLVREMVDSDWRLAQRDALVEREGFAAYKHHE"
     gene            2927..>3210
                     /locus_tag="VFL84_02555"
     CDS             2927..>3210
                     /locus_tag="VFL84_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7607532.1"
                     /translation="MNKEASVFVAGARGLVGSAICRALAARGFARVLAPSRSTLDLRD
                     RAAVDRFFTAERPDHVFLAAAKVGGIVANDTYPADFIRDNLEIQTNVIDAA"
BASE COUNT          536 a         1087 c         1044 g          543 t
ORIGIN      
        1 cacagcatgt acgggagcgg ccgcttgggc tttgttgccg gtcggcgcgc aactccctaa
       61 agtgctcacg acgcggcgtg tggatggaat agacgtgtgt gggttcatcg gcggaacgga
      121 tcccgactgg agttacgccg cggccctcgc ctcgatcgcg catcgcggcc cggacgccgc
      181 attgctgtcg ctcgacggcc cggtcaaagt cggttttcgt cgcctatcga tcatcgatct
      241 gcgtccgtcg gccaatcagc cgatgctcgc gcccgacggc gcgagctgga tcgtcttcaa
      301 cggtgagatc tacggatacc gggagctgcg cagcacgctc cagaaacgcg gccgcgtctt
      361 cctgaccgac tccgacacgg aagtcgcgct gcacgcctac ctcgaatggg gcgccgactt
      421 cgtcgaccga atcgacggca tgttcgcgat cgtcatctgg gacgcgcgcg accggaagct
      481 caggctctat cgcgaccgcc cgggtatcaa gcctctctat tattactacg acggcagcag
      541 attcgcgttc gcgtcggagc tcaaggccct cgaggcggcg ctgcgcccgg gcgagctcgc
      601 gatcgacggc accgcgtgct acgactttct cggctaccgg tacgtgcccg cgccgaagac
      661 gctgtacaag cgctgtttca agctcgagcc cgcgcacgag ctcgtctacg cgccggacac
      721 gggctcactc tccgcgcctc gccggtactg gagcttgccg attccggcgg agccgcgtgc
      781 gccggccgtc gaggccgcct gcgaggagct acgctcgctc gtcgccgcgt cggtcgtgga
      841 gcagatgatc gccgatgtgc cgctcggctt cttcctgtcc ggcggcgtcg attcgagcgt
      901 cgtcgttgcc gccgcggctg cgaccggcac gcgcgtctcc acgttctcga tcgggttcga
      961 cagcgacgag gtatcggaga cgccgttcgc gcgcgaggtc gcgcgacgct tcgcgaccga
     1021 tcatcacgag cgcatcctgt cgcaagcgca cgcgcaggag ctgctgccgc ggctcaaagc
     1081 gtggttcgac gagccgttcg ccgacgagtc gtcgatgccg acgtacctcg tttgcgcagc
     1141 ggcgcgcgag cacgtgaccg tcgtgctgac gggcgacggc ggcgacgagg tgttcggcgg
     1201 ttatcgcacg tacccgcgct atgctcgcta cgcgcgctgg ccgacgtggc ccgccgcgat
     1261 ggaacgcgcc gcctacgcgc tgcgccggcc gtttccgcgc cgccacgccg tcgcgcgctt
     1321 cgtgacgctg ctcgaaacgg ccttgagcag cggcccgaat ctctgggcga agctcatggg
     1381 cggaatgtcg acgcccgcga aacgcgctta cgcgagcgag ctcgggattc cggccgacta
     1441 cgacgattgg tggcactacc gcgagcattg gcgcgacgac ttgccgctgc gcacgcggct
     1501 gcaatacgtc gacttccaca cgttcatgcc cggcctcgtg ctaaccaagg tcgaccgcac
     1561 gagcatggcc gtgtcgctcg aagcgcgcgt gccgttgctc gcgcgccgcg tcatcgagtt
     1621 ttcgttcggc ctcgacgagg acctgcgcta ctacggcggc gagccgaaag gcttgctgcg
     1681 ccaggcttac cgcggcatcc tgcccgacca catactcgat cgccgcaaga aagggttcgg
     1741 cattccgcgt tactacttga aggacgtcgg cggcggcagg ttgattcaag agcacgttct
     1801 acgcagcttg tttctaggag cgacggttga aaactgaggg aaaaggcaag aaggcgttga
     1861 tcacgggcat cacgggccag gacggcgcgt atttggccga gtttctgctc gcgaagggtt
     1921 acgaggttca cggcatcaag cggcgcgcgt cgtcgttcaa cacggaccga atcgaccacc
     1981 tctatcgcga cccgcacgag cccgatcgca aaatgattct gcactacggc gacctcacgg
     2041 acgccacgaa cctgattcgc gtcgtgcagg aggttcggcc gcaggaaatc tataacctcg
     2101 cggcgcagag ccacgtcgcc gtctcgttcg aaacggcgga gtacacggcg aacgccgatg
     2161 cgctcggcac gctgcggctg ctcgaggcga tcaggatctg cgagctcggc gccaagacga
     2221 ggttctatca agcctcgagc tcggagatgt acggcaaagt cgtcgagacg ccgcagcgcg
     2281 agacgacgcc gttctatccg cgctcgccgt acgcggctgc caaggtctat gcgtattgga
     2341 tcacggtgaa ttaccgtgag gcctacggct tgtacgcctg caacggcata ctcttcaacc
     2401 acgagagtgc cacgcgcggc gagaccttcg tcacgcgcaa gatcacgcgc ggactcgcgc
     2461 gcatcaaggc gggtctcgac gactgcctgt acttgggcaa cctagacgcg ctgcgcgact
     2521 ggggtcacgc gcgcgactac gtgcgcgcgc agtggctgat gctgcagcaa gacaagcccg
     2581 aggactacgt catcgcgacc ggcgagcagc attccgtgcg cgagttcgtc gagcgcgcgg
     2641 cccaggcgct cggcatgcag cttcgctggc acggctcgaa gctcgacgag cacgccgtcg
     2701 aagcaagctc cggccgcacc gtcgtgcgca tcgatccgcg ttatttccgc ccgaccgagg
     2761 tcgacacgct gctcggcgat gcctcgaaag cgcgcaagca gctcggctgg gtgcccgaga
     2821 tcggtttcga ccagctcgtg cgcgagatgg tcgactcgga ttggcggctc gcgcaacgcg
     2881 acgcgctcgt cgagcgcgaa ggctttgcgg cctacaagca tcacgaatga acaaggaagc
     2941 cagcgtcttc gtggccggcg cacgcgggct cgtgggctcc gcgatctgcc gcgcgctcgc
     3001 cgcccgaggc ttcgctcgcg tgctcgcgcc gagccgcagc acgctcgatc tgcgcgaccg
     3061 cgccgccgtc gaccgcttct ttacggccga gcgtcccgac cacgtattcc tggcggcggc
     3121 gaaggtcggc ggcatcgtgg ccaacgacac ttatcccgcc gacttcatcc gcgacaacct
     3181 cgagatccag accaacgtca tcgacgcggc
//
LOCUS       DASSNN010000145         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_29358, whole genome shotgun sequence.
ACCESSION   DASSNN010000145 DASSNN010000000
VERSION     DASSNN010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29358"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VFL84_02560"
     CDS             complement(<1..1227)
                     /locus_tag="VFL84_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019018573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HET7607533.1"
                     /translation="MADDSPRFDLSAPLDELHSNERVKHDSRYLRGTLAESLADAATG
                     AVDETDTLLTKFFGVYQQDDRDLRAERRRAKLEPLYQFMVRVRLPGGVCTPAQWLALD
                     ALARDFADGTLRLTTRQTFQFHGIRKKNLRKHLHGLNAAGLDTVAACGDDNRNVICTA
                     NPLRSPAHAAVSQLALRIGAHLLPRTGAYREILLGEPPAAPPGGNEEPLYGPTYLPRK
                     FKIALAVPPRNDVDVYAHDLGFVAVVAGGRVTGYNVLVGGGMGMTHNVPATFPRLAEA
                     VGFCLPEDVVDVAEHTLAIQRDFGNRKDRAHARFKYTIEDRGTEWFRGELERRLGRAL
                     ENARAVRFESSADDLGWTEGADGRWHYGLFVENGRVADRPGVALLGGMRAIAAAHRGL
                     FAITTNQNLVIAGVTAD"
     gene            complement(1227..3047)
                     /locus_tag="VFL84_02565"
     CDS             complement(1227..3047)
                     /locus_tag="VFL84_02565"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HET7607534.1"
                     /translation="MSAGKRELGARPAGARPHDDAWRAAPFSRRDAEEVRALVARLAP
                     EQRLWLSGYLLGSAAQPVAAAAAPAGDAVTVLFGSQSGNSERLASRLAERLAAAHTPH
                     RLLDMLDCRKADLEQARVLLVIVSTHGDGEPPERAVPLHELLHGRKPPRLDDVRYAVL
                     ALGDSSYEQFCATGRVFDARLAELGAQPLQPRTECDVDFETEAASWIEAVVAKVAADA
                     HTAAPAMTAERHSAAVTHAYTRKNPFAAELLVNQRLTARGSSKDVRHLELSLDASSMH
                     YEPGDSLGVVPRNSAAEVDALLAALRFDPEAPVPTEPGELPLRTALVERYEIGVLGAQ
                     LVRRYAGATGDAELERLAADADAFKRFARGRDLRDLVAQRPPRGLDPAGFVRLLQPLA
                     PRLYSIASSAKVTPDEAHLTVGVVGYESLGRARRGVVSGALAEVAEGDSVPVYLQRNT
                     GFRLPSDPSAAIVMIGAGTGIAPFRAFVAEREALDAAGRHWLVFGDRSFELDFLYQAD
                     WLAWRKRGVLARLDVAFSRDQASKIYVQHRLREHGAELWAWLQDGAHLYVCGDAEHMA
                     PDVEQALLDVARRHGGLKDDAASEYLLELQRARRYQRDVY"
     gene            complement(3071..>3286)
                     /locus_tag="VFL84_02570"
     CDS             complement(3071..>3286)
                     /locus_tag="VFL84_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HET7607535.1"
                     /translation="PASTGRNFDEILRLIDSLQLTDKHKVATPVNWRSGDDVIIVPSL
                     PLEEAKRLFPSGWNEQRPYLRTVKQPA"
BASE COUNT          472 a         1138 c         1198 g          478 t
ORIGIN      
        1 gtccgccgtc acgcccgcga tgacgaggtt ctgattcgtc gtgatcgcga acaggccgcg
       61 atgcgcggcc gcaattgcgc gcatcccgcc gagcagcgcc acgccgggcc ggtcggcaac
      121 ccggccattc tcgacaaaca agccgtagtg ccagcggccg tcggcgcctt cggtccagcc
      181 gagatcgtcg gccgacgact cgaaacgcac cgcgcgcgcg ttctcgagcg cgcggccgag
      241 ccgccgctcg agctcgccgc ggaaccactc cgttcctcga tcctcgatcg tgtacttgaa
      301 gcgcgcgtgc gcgcggtcct tgcggttacc gaagtcgcgc tgaatcgcga gcgtgtgctc
      361 ggcgacgtcg acgacgtcct cgggcagaca gaagccgacg gcctctgcga ggcggggaaa
      421 cgtggccggc acgttgtgcg tcatgcccat gccgccgccg acgagcacgt tgtagccggt
      481 cacgcggccg ccggcgacga ccgcgacgaa gcctaagtcg tgcgcgtaca cgtccacgtc
      541 gttgcgcggc ggcacggcga gcgcgatctt gaacttgcgc ggcagatacg tggggccgta
      601 cagcggctcc tcgttgccgc cgggcggcgc ggccggcggc tcgccgagca ggatctcgcg
      661 atacgcgccg gtgcgcggca gcaagtgcgc gccgatccta agcgcgagct ggctcacggc
      721 ggcgtgcgcg ggagaccgca atgggttcgc ggtgcagatc acgttgcgat tgtcgtcgcc
      781 gcaagccgcg acggtgtcga gccccgcggc gttgagaccg tggaggtgct tgcgcagatt
      841 tttcttgcgg atgccgtgga actgaaacgt ctggcgcgtc gtgaggcgca gcgtgccatc
      901 ggcaaagtcg cgcgcgagcg cgtcgagcgc gagccattgc gcgggagtgc agacaccgcc
      961 cggcagccgc acgcgcacca tgaactggta aagcggctcg agcttcgcgc ggcggcgctc
     1021 ggcccgcaga tcgcggtcgt cttgctggta gacgccgaag aacttcgtga gcagcgtatc
     1081 cgtctcgtcg acggcgccgg tcgccgcgtc ggcgaggctt tcggcaagcg tgccgcgcag
     1141 atagcggctg tcgtgcttga cgcgctcgtt cgagtgcagc tcgtcgagcg gcgccgacag
     1201 atcgaaacgc ggcgagtcat ctgccatcag taaacgtctc gctggtagcg ccgagcccgt
     1261 tgcagctcga gcaggtattc gctcgccgcg tcgtccttga ggccgccatg gcgacgcgca
     1321 acgtcgagca gcgcttgctc gacgtccggg gccatgtgct cggcgtcgcc gcagacgtag
     1381 agatgcgcgc cgtcctgcag ccacgcccag agctcggcgc cgtgctcgcg caaacggtgc
     1441 tgcacataga tcttgctcgc ctgatcgcgc gagaacgcga cgtcgagccg cgcgaggacg
     1501 ccgcgtttgc gccatgcgag ccagtcggcc tgatacagaa aatcgagctc gaacgagcgg
     1561 tcgccgaaca cgagccagtg gcggcccgcg gcgtcgagcg cctcgcgctc ggcgacgaac
     1621 gcgcgaaatg gcgcgatgcc ggtgccggcg ccgatcatca cgatcgcggc gctcgggtcg
     1681 gacggcagac gaaagcccgt gttgcgctgc aaataaaccg gaacggaatc gccctccgcg
     1741 acctcggcga gcgcgcccga gacgacgccg cgccgagcgc ggcccagcga ttcgtagccg
     1801 acgacgccga ccgtgaggtg cgcttcgtcc ggtgtcacct tggcgctcga cgcgatcgag
     1861 tagagccgcg gcgcaagcgg ttgtagcagg cgcacgaagc cggccggatc gaggccgcgc
     1921 ggtgggcgct gcgcgacgag gtcgcgcagg tcgcggccgc gcgcgaaacg tttgaacgcg
     1981 tccgcgtcgg cggcgaggcg ctcgagctcg gcgtcgcccg tcgcgcccgc gtagcggcgg
     2041 acgagctgcg cgccgagtac tccgatctcg tagcgctcga cgagtgccgt ccgcagcggc
     2101 agctcgccgg gctcggtggg cacgggggcc tctggatcga agcgcaaggc cgcgaggagt
     2161 gcgtccacct cggcggcgga gttgcgcggc acgacgccca aggagtcgcc cggctcgtag
     2221 tgcatgctcg acgcgtcgag cgacagctcg aggtggcgca cgtccttgct cgagccgcgc
     2281 gccgtgagcc gctggttgac gaggagctcg gcggcgaatg ggttcttgcg cgtgtacgcg
     2341 tgcgtcacgg cggccgagtg ccgctcggcc gtcatggccg gtgccgccgt gtgcgcgtcg
     2401 gccgcgacct tcgcgacgac ggcctcgatc cagctcgccg cctcggtctc gaaatcgacg
     2461 tcacattccg tgcgcggctg tagcggctgc gcgccgagct ctgcgaggcg cgcgtcgaaa
     2521 acccggccgg tcgcgcagaa ttgctcgtag ctcgagtcgc cgagcgcgag caccgcgtaa
     2581 cgcacgtcgt cgagccgcgg cggcttgcgg ccgtgcagca gctcgtgcag cggcacggcg
     2641 cgttcgggcg gctcgccgtc gccgtgcgtg ctcacgatca cgagaagcac gcgtgcctgc
     2701 tcgaggtcgg cctttcggca gtcgagcatg tcgagcagcc tgtgcggcgt gtgcgccgcc
     2761 gcgagccgct ccgcgagccg gctcgcgagc cgttccgagt tgccgctctg cgagccgaac
     2821 aggaccgtga cggcgtcgcc cgcgggagcc gcggcggccg cgacaggctg cgccgcggag
     2881 cccaagaggt agccgctcag ccagaggcgc tgctcgggcg cgagccgcgc gacgagcgcg
     2941 cgcacctcct cggcgtcgcg ccgcgagaac ggcgcggcgc gccacgcgtc gtcgtgcggc
     3001 cgagcgcctg cgggtcgtgc gccgagctcg cgcttcccgg cgctcatctc tgccgccacc
     3061 ccggccacct tcaagcgggc tgcttcaccg tgcgcaggta cggccgctgc tcgttccagc
     3121 cgctcggaaa caagcgcttc gcctcctcga gagggagcga cggcacgatg atgacgtcgt
     3181 cgccggagcg ccagttcacc ggcgttgcga ctttgtgctt gtccgtgagc tgcagcgagt
     3241 cgatgagacg caggatctcg tcgaagttgc ggcccgtgct cgcggg
//
LOCUS       DASSNN010000146         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1275517, whole genome shotgun sequence.
ACCESSION   DASSNN010000146 DASSNN010000000
VERSION     DASSNN010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275517"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2155
                     /locus_tag="VFL84_02575"
     CDS             <1..>2155
                     /locus_tag="VFL84_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HET7607536.1"
                     /translation="GQRRYLESLNAYARQFVEPAGRADVDAVYGIPPTVAIEQRTSRG
                     GRKSTVATLTEVYHYLRLLFVKLGVQHCPDCGVPIDAQSLASIHAKVMKRYRGRRISL
                     LAPLVIGRKGYYTELAEWAAGRGYSTLRVDGVATPTDRWPRLDRFKEHDIELPVAELD
                     VKPAAERELRDALARAVELGRNMVRVLDASAGGEDGELFSTERACPKCSRSFEPLDPR
                     LFSYNSRYGWCPECFGTGVDLSGFDAEQSGEESEWAEVAGDAEACAACGGSRLKPEAL
                     AVRFHGQTIAEVTQRSIAASLAYFEKLKLEPREREIARDVTPELVSRLGFLDLVGLGY
                     LTLDRAAPTLSGGEAQRIRLAAQLGSNLRGVCYILDEPTIGLHPRDNALLLATIEKLA
                     AKGNTVVVVEHDEETIRRAQHVVDLGPGAGRNGGEVVVNGTLADLVASERSLTGQALA
                     TPPRHPLRERRKTHKNGGIGTAWLELEGVTRNNLRGLDVALPLARLVCVTGVSGSGKS
                     TLLRDVLEDNLARLVAARRGRAPQLGSLKSLKGWQGISRVLEVDQTPIGKTPRSCPAT
                     YVGIFDEIRKLFAQTTEARLRGYDASRFSFNASGGRCEGCEGQGLKKIEMSFLPDVRV
                     DCERCGGKRFNSETLAVQFRGKSIGDVLTMSVDEAAAFFAAQPKIHHPLTLLRDVGLG
                     YLGLGQPSPTLSGGEAQRVKLVTELAKVRPESLRAR"
BASE COUNT          330 a          729 c          784 g          312 t
ORIGIN      
        1 aggggcagcg ccggtatctc gagtcgttga acgcgtacgc gcggcagttc gtcgagccgg
       61 cggggcgcgc cgacgtggat gctgtctacg gcattccgcc gaccgtggcg atcgagcagc
      121 gcacgagccg cgggggacgc aagagcaccg tcgcgacgtt gacggaggtc tatcactacc
      181 tgcgcctgct gttcgtgaag ctcggcgtgc agcactgccc ggactgcggc gtgccgatcg
      241 atgcgcagag cctcgcgtcg atccacgcga aggtcatgaa gcggtatcgc ggccgacgca
      301 tttctctgct cgcgccgctc gtcatcgggc gcaagggtta ttacaccgag ctcgccgagt
      361 gggcggcggg ccgcggctat tcgacgttgc gcgtcgacgg cgtggcgacg ccgacggacc
      421 ggtggccgcg cctcgaccgg ttcaaggagc acgacatcga gctgcccgtc gccgagctcg
      481 acgtgaagcc cgccgcggag cgcgagctca gggacgcgct cgcgcgcgcc gtcgagctcg
      541 gccgcaacat ggtgcgcgtg ctcgacgcgt cggcgggcgg cgaggacggt gagctcttct
      601 cgaccgagcg cgcgtgtccg aagtgcagcc gcagcttcga gccgctcgat ccgcgtctct
      661 tctcgtacaa ctcccgctac ggctggtgcc ccgagtgctt cggcacgggc gtcgatcttt
      721 ccggtttcga cgccgagcag agcggcgagg aaagcgagtg ggccgaggtc gccggcgatg
      781 cggaggcgtg cgccgcgtgc ggaggctcga ggctcaagcc cgaagcgctc gcggtgcggt
      841 ttcacggcca aacaatagcc gaggtcacgc agcgttcgat cgcggcgtcg ctcgcgtatt
      901 tcgagaagct gaagctcgag ccgcgcgagc gcgagatcgc gcgcgacgtg acgccggagc
      961 tcgtcagccg gctcggcttc ttggacctcg tcggcctcgg ctacctgacg ctcgaccgcg
     1021 ccgcgccgac gctgtcgggc ggcgaagcgc agcgcatcag gctcgcggcg cagctcggct
     1081 cgaacctgcg cggcgtctgc tacatcctcg acgagccgac gatcggcttg catccgcgcg
     1141 acaacgcgct gctgctcgcg acgatcgaga aactcgcggc gaaaggcaac acggtcgtcg
     1201 tcgtcgagca cgacgaggag acgatccgcc gggcgcagca cgtcgtcgat ttgggcccgg
     1261 gagcggggcg caacggcggc gaggtcgtcg tcaacggcac gctcgccgat ctcgtcgcga
     1321 gcgagcgctc gctgaccggg caagcgctcg ccacgccgcc gaggcatccg ctgcgcgagc
     1381 gccgcaagac gcacaagaac ggcggcatcg gcaccgcgtg gctcgagctc gaaggcgtga
     1441 cgcgcaacaa cctgcgcggc ctcgacgtcg cgctgccgct cgcgcggctc gtctgcgtca
     1501 cgggggtcag cggcagcggc aagagcacgc tgctgcgcga cgtgctcgag gacaatctcg
     1561 cgcggctcgt ggccgcgcgc cgcggccggg cgccgcagct cggctctttg aagagcttga
     1621 aaggttggca agggatcagc cgcgtgctcg aggtcgacca gacgccgatc ggcaagacgc
     1681 cgcgctcgtg cccggcgacc tacgtcggca tcttcgacga gatccgcaag ctgttcgcgc
     1741 agacgacgga agcgcggctg cgcggctacg acgcgagccg cttctcgttc aacgcctcgg
     1801 gcgggcgctg cgagggctgc gaagggcagg gcctcaagaa gatcgagatg agcttcctgc
     1861 ccgacgtccg cgtcgattgc gagcggtgcg gcggcaagcg cttcaactcg gagacgctcg
     1921 ccgtgcaatt tcgcggcaag agcatcggcg acgtgctgac gatgagcgtc gacgaggccg
     1981 ccgctttctt cgctgcgcag ccgaagatcc atcacccgtt gacgctgctg cgcgacgtcg
     2041 gcctgggcta cttgggcctc gggcagccga gcccgacgct gtcgggcggc gaggcgcagc
     2101 gcgtgaagct cgtcaccgag ctcgccaagg tgcggcccga atcgctgcgt gcgcg
//
LOCUS       DASSNN010000147         3657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_408259, whole genome shotgun sequence.
ACCESSION   DASSNN010000147 DASSNN010000000
VERSION     DASSNN010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408259"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VFL84_02580"
     CDS             <1..514
                     /locus_tag="VFL84_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607537.1"
                     /translation="GPVPRLGENVPRWYGETIGFWDEDTLITWTSNIQGWATHSAFEH
                     SNQMQSIEIYTPLHDENGVFVGLNHEAILYDPEALVQPIRIMYRLMKQADFADPNINP
                     YVFTECVQTIFPIDGTATAVTPGRVIEYEVPDMYGRPWAHIWSKYWEQGMQGSENEDI
                     FDFGDNGAGR"
     gene            526..1641
                     /locus_tag="VFL84_02585"
     CDS             526..1641
                     /locus_tag="VFL84_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7607538.1"
                     /translation="MRQASILVIGAGIGGLTAAIALGRRGFHVSMIERDPEWSVYGVG
                     IIQQSNVVRAMHMLGILKDYLDAGVGFDHVEIYAPDGRRLARVPSPKLVPEYPANVGI
                     GRRALQGVLARRAREAGAQVKLGVEATRLDDDGDGVTVQFSDGSTARYDVVVGADGLY
                     STTRAMLFPEAIGPELTGQGVWRYNFPREPGLDALQAYEGPIGMGLVPLNKDLMYMYV
                     TSPEPKNPRYPRAHLAQAMRAKLKNAPPRIAALAEQITDDAGVVYKPLEWLYLDGPWH
                     KGRVALLGDAVHATTPHLGQGAGMAIEDGIVLAEELARADAPQAAFTAWQARRVERCR
                     YIVEQSKQIGDSQLGLRPPVDQSAAVRGMFEMIARPL"
     gene            1666..2568
                     /locus_tag="VFL84_02590"
     CDS             1666..2568
                     /locus_tag="VFL84_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7607539.1"
                     /translation="MAAVTDIRFVGYGVPDLPVERAFYRDKWGLAEAGEQAGLVYFAV
                     EGSREPCAVRLRAADTRRIDVIELAAATRADVDALHGQVLAAGCRVIFAPRELTGPFG
                     GYGFRFFSPDGLPFGISSDVAQRTARDVGPLESVPVRISHIVLHSPDHQTMVRFFTDV
                     LGFRISDWLGDFMCFLRCNEWHHRIALLPGPPCLNHVAYDMASLDAMMRGVSRLRAEG
                     IDVQWGPGRHKAGNNTFSYFVTPNGFAVEYTAELDRVDEATWQPTVYKPEPGLMDLWG
                     TGVGGPQSMPRPAPDAGLFQPAES"
     gene            2570..>3657
                     /locus_tag="VFL84_02595"
     CDS             2570..>3657
                     /locus_tag="VFL84_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET7607540.1"
                     /translation="MALFQYFPSNYVWNLAVNMALESGGQIGEIEDMCGPLREAAARG
                     NDAGTAEFLEQWEKMADKLLELAAEDAARGRLLSASRKGQRAALYLIVAERMQGRGHP
                     RREATFARAQQAFRHAVNIGGENAERVEIPYEGKSLPALLTRPPGARGKLPCVLYVNG
                     LDSCKELLYWSWLPRELARRGIATLSVDQPGTGEALRLSGLPATAASERWATPCYEWL
                     AARADIDANRIGMTGISLGGYFTPRAMAFEPRFASGAVWGANHDWHEVQHKRLLREGE
                     NPVPHYWNHVQWVFGARDRDEFFAKARDMCLDGILDRIRVPFLVTHGEKDRQIALAYA
                     HRTFEQLVNSPRRELKVFTAREGGVEHVG"
BASE COUNT          601 a         1224 c         1240 g          592 t
ORIGIN      
        1 cggccccgtg ccgcgcctcg gcgagaacgt gccgcgttgg tacggcgaga cgatcgggtt
       61 ctgggacgag gacacgctga tcacgtggac gtcgaatatt cagggctggg ctacgcactc
      121 ggcgttcgag cactcgaacc aaatgcagtc catcgagatc tacacgccgc tccacgacga
      181 gaacggtgtc ttcgtggggc tcaaccacga agccatcctc tacgaccccg aggcgctcgt
      241 ccaaccgata cgcatcatgt atcgcctgat gaagcaggcg gacttcgccg atcccaacat
      301 caacccctac gtcttcaccg aatgcgtgca aacaattttt cccatcgacg gcactgccac
      361 cgccgtgacg ccgggccgcg tgatcgaata cgaagtgccc gatatgtatg gacgcccgtg
      421 ggcgcacatt tggagtaagt actgggagca gggcatgcag ggcagcgaga acgaggacat
      481 cttcgacttt ggggacaacg gcgccggccg ctgagaggca aagcattgcg acaggctagc
      541 atcctcgtca tcggcgccgg catcggcggt ctcacggcgg ccattgcgct cggccggcgc
      601 gggttccacg tgagcatgat cgaacgcgat cccgagtgga gcgtgtatgg ggtcggcatc
      661 attcaacaat cgaacgtagt ccgcgcgatg cacatgctcg gcatcctcaa ggactatctc
      721 gatgccggcg tcgggttcga tcatgtggaa atctacgcgc cggacggccg ccgcttggcg
      781 cgcgtccctt cgccgaagct cgtgccggag tatccggcca acgtcggcat cggccgccgt
      841 gcgctgcagg gcgtactggc gcgacgcgcg cgcgaggccg gtgcgcaggt gaagctcggc
      901 gtcgaggcca cgcggctcga cgacgacggc gacggcgtca ccgtgcagtt ttcggacggc
      961 agcaccgcgc gctacgacgt cgtcgtcggc gccgacgggc tctattcgac gacgcgcgcc
     1021 atgctgttcc ccgaggcgat aggtcccgag ctcacggggc agggcgtgtg gcgctacaac
     1081 ttcccgcgcg agccggggct cgatgcgctg caggcctacg agggcccgat cggcatgggc
     1141 ctcgtgcctc tcaacaagga cctcatgtac atgtacgtta cgtcgccgga gcctaagaat
     1201 ccgcgttatc cgcgcgcgca tttggcgcag gcgatgcgcg ccaagctgaa gaacgcaccg
     1261 ccccgcatcg ccgcgctggc cgagcagatc accgacgatg cgggcgtcgt ttacaagccg
     1321 ctcgaatggc tctatctcga cgggccctgg cacaaggggc gcgtagcgct gctcggcgat
     1381 gccgtgcatg ccacgacgcc gcacctcggg cagggcgccg gcatggcgat cgaggatggt
     1441 atcgtgttgg ccgaggagct cgcgcgtgca gacgcgccgc aagccgcgtt caccgcgtgg
     1501 caggcgcgcc gcgtcgagcg ctgccgctac atcgtcgaac aatcgaagca gatcggcgac
     1561 agccagctcg gcttgcgtcc gcccgtggat cagagcgccg cggtgcgcgg catgttcgag
     1621 atgatcgcgc gtcctctgtg atgcgccgcc gtattggagt taccgatggc cgccgtgacc
     1681 gacatccgtt tcgtgggcta tggagtgccc gacctccccg tcgagcgcgc gttctaccgc
     1741 gacaaatggg gcctcgccga ggccggcgag caggcggggc tcgtctactt cgccgtcgaa
     1801 ggtagccgtg agccttgcgc agtgcggctg cgcgcggcgg acacgcggcg catcgacgtg
     1861 atcgagctcg ccgcggcgac tcgcgccgac gtcgacgcgc tgcacggcca ggtgctcgcc
     1921 gcgggctgcc gcgtgatctt cgcgccgcgc gagcttacgg gtccgttcgg cggctacggc
     1981 tttcgcttct tctcgccgga cgggctgccg ttcggaattt cgtccgacgt cgcgcaacgg
     2041 acggcgcgcg acgtcggtcc gctcgagtcc gtgcccgtgc gcatcagcca catcgtgctc
     2101 cactcgccgg accatcagac gatggtgcgg ttctttaccg acgtcctggg ctttcgcatc
     2161 agcgattggc tcggcgactt catgtgcttc ttgcgctgca acgaatggca tcaccgcatc
     2221 gcgctgttgc cggggccgcc ttgcttgaac catgttgcgt acgacatggc gagcctcgat
     2281 gccatgatgc gcggcgtgtc gcgcctgcgc gcggagggca tcgacgtgca gtgggggccg
     2341 ggccgccaca aggccggcaa caacacgttc agctacttcg tcacgccgaa cggctttgcc
     2401 gtcgaataca cggccgagct cgaccgcgtg gacgaggcca cctggcagcc cacggtctac
     2461 aaacccgagc caggactcat ggacctctgg ggcaccggcg tcggcgggcc gcaatcgatg
     2521 ccgcgccccg cgcccgatgc gggactgttt cagcccgccg agtcttgaga tggccctgtt
     2581 ccaatacttc ccgagcaact acgtctggaa tctcgccgtc aacatggcgc tcgagagcgg
     2641 cggccagatc ggcgagatcg aggacatgtg cgggccgctg cgcgaagccg ccgcacgcgg
     2701 caacgacgcc ggcacggccg agttcctcga gcagtgggaa aagatggcgg acaagctgct
     2761 cgagctcgcc gccgaggacg cagcgcgcgg gcgattgctg tccgcctcgc gcaaagggca
     2821 acgggccgcg ctctatctga tcgtggcgga gcgcatgcag ggccgtgggc acccgaggcg
     2881 cgaagcgacg ttcgcacgcg cgcagcaagc gttccgccac gccgtaaaca tcggcggcga
     2941 gaacgcggag cgcgtcgaga ttccgtatga aggcaagagc ttgcccgcgc tcctgacgcg
     3001 tccgcccggc gcgcgcggca agctgccctg cgtgctctac gtcaacggcc tcgacagctg
     3061 caaggagctg ctgtactggt cctggctgcc gcgcgagctc gcacggcgcg gaatcgccac
     3121 gctctcggtc gatcagccgg gtaccggcga agcgttgcgg ctgtcggggc ttcccgccac
     3181 ggcggccagc gagcgctggg ccacgccttg ctacgagtgg ctcgccgcgc gcgccgacat
     3241 cgatgcgaac cgcatcggca tgaccggcat ctcgctcggc ggttacttca cgccgcgcgc
     3301 catggccttc gagccgcgct tcgcgagcgg cgcggtgtgg ggcgcgaatc acgattggca
     3361 cgaggtgcag cacaagcgtc tgctacgcga gggcgagaac cccgtgccgc attactggaa
     3421 tcacgtgcag tgggtttttg gcgcgcgcga tcgcgacgag tttttcgcga aggcgaggga
     3481 catgtgcctc gacggcatcc tcgaccgcat ccgtgtgccg tttctcgtca cgcacggcga
     3541 gaaggaccgg cagatcgcgc tcgcgtacgc gcaccgcacg ttcgagcaac tcgtcaactc
     3601 gccgcggcgc gagctcaaag tgttcacggc gcgcgagggc ggcgtcgagc acgtcgg
//
LOCUS       DASSNN010000148         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1038849, whole genome shotgun sequence.
ACCESSION   DASSNN010000148 DASSNN010000000
VERSION     DASSNN010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038849"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..911
                     /locus_tag="VFL84_02600"
     CDS             222..911
                     /locus_tag="VFL84_02600"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET7607541.1"
                     /translation="MPSTRALLAALALMPYAAAGQPTPTPAAAPWFAVGYVETQASKA
                     EAGSAALANYRAALERQPGCVAVELFAQAGRPGHFAVLETWRDQAAFDGRDIDAKRQL
                     MGALAPIRVSDYDERPYKPLTLAPAKSTAPDTRGAFVVAHVDVAPNSAAPMLLLALAE
                     ASRQEPGNLRFDVLQHAMRGNHFTVIEQWRNRDALDAHVGTAHARAYRDALQPLTGSP
                     LDERVYTPIAP"
     gene            complement(930..2066)
                     /locus_tag="VFL84_02605"
     CDS             complement(930..2066)
                     /locus_tag="VFL84_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HET7607542.1"
                     /translation="MLALRMRQKNAEFYFVSYPAEDLLKHVRFETRFYGDRGEVVGGD
                     ASGKDAEEIDDVERYVRAIERRAGAFQRELNRRKVRQIRDFFRNEADQPVVPGAVLLF
                     TSEELAFSKLGQYAQMGDLSMPRSPFLIIDGQHRLAGLHFYLREPDADRRVEVPCVIF
                     DGKTADFATEMFVIINSTHTRINKSHLVDLYEKIEWGTDPHKKLAARIVQALYSESDS
                     PLRYHVNMLGGRSQQDMWINQAQLYGEVHRFVTRKDTQGWTTDGRGLSRDRAYGFLRD
                     VLRAAKRAFGDAWGDNKRYMVTRDVTLKALVRVAGDAAAKTRAPVAMSASSEMENALA
                     KRFAPWSELVGEFRRDGFYERFPARGQVERVEKIRARLVREAGL"
BASE COUNT          338 a          765 c          702 g          341 t
ORIGIN      
        1 ccgccgcttc cgaatggtga cccaaagccg gcatggcggc tgcaaggccc acggccgccg
       61 gcagcagcaa caacgctcgc gtcatcgcca atctccccct attcgccgga ctactttgcc
      121 tcaagccgtg cacggactgg aataggccgg gctagccgtc gcgccgcgac ctcgagcatg
      181 atctcgcccg tgccgcagcc aaccagagtg aggtcgcccc gatgccgtcc actcgcgctt
      241 tgctcgccgc cttggcgctg atgccatacg cggccgccgg tcagccgacc cccacacccg
      301 ccgcggctcc ttggttcgcc gtcggttacg tcgagacgca ggcgtccaaa gcggaggccg
      361 gaagcgcggc gctcgcgaac tatcgcgccg ccctggagcg gcagccgggc tgcgtcgcgg
      421 tcgagctgtt cgcgcaggcg ggccggccgg gccacttcgc ggtgctcgag acctggcggg
      481 accaggccgc attcgacggc cgcgatatcg acgcgaagcg gcagctcatg ggagcgttgg
      541 cgccgatccg cgtcagcgat tacgacgagc ggccctacaa acccctgacg ctcgcgccgg
      601 cgaagagcac ggcgcccgac acgcgcggcg cgttcgtcgt cgcgcacgtc gacgtcgcgc
      661 cgaacagcgc cgcgccgatg ctgttgcttg cgctcgcgga ggcgagccgg caagagcccg
      721 gcaacctgcg cttcgacgtg cttcagcacg cgatgcgcgg caatcacttc acggtcatcg
      781 agcagtggcg caaccgagac gcgctcgacg cgcacgtcgg caccgcgcac gcgcgcgcct
      841 atcgcgacgc gctacagccg ctgacgggca gcccactcga tgagcgcgtc tacacgccga
      901 tcgcgcccta gtgcgcagcg ttcacgcttt cagaggccgg cttcgcggac cagccgggcg
      961 cggatcttct cgacgcgctc gacctggccc cgcgccggaa aacgctcgta gaacccgtcg
     1021 cgccggaact cgccgacgag ctcggaccag ggtgcgaacc gcttcgcgag cgcgttttcc
     1081 atctcggagc tcgcggacat cgctacgggc gcgcgcgtct tcgccgcggc gtcgccggcc
     1141 acgcgcacga gggctttgag cgtgacgtcg cgcgtgacca tgtagcgctt gttgtcgccc
     1201 cacgcgtcgc cgaacgcgcg tttggccgcg cgcagcacgt cgcgcaaaaa cccgtacgcc
     1261 cggtcgcgcg agagtccccg cccgtccgtc gtccatcctt gggtgtcttt gcgagtgacg
     1321 aaccgatgga cctcgccgta gagctgcgcc tggttgatcc acatgtcttg ctgcgaccgg
     1381 ccgccgagca tgttgacgtg gtagcgcagc ggcgagtcgc tctcggagta cagcgcctgg
     1441 acgatgcgcg cggcgagctt cttgtgcgga tcggtgcccc actcgatctt ctcgtaaagg
     1501 tcgacgaggt ggctcttgtt gatgcgcgtg tgcgtggaat tgatgatcac gaacatctcg
     1561 gtcgcgaagt ccgcggtctt gccgtcgaag atcacacacg gcacctcgac gcggcggtcg
     1621 gcgtcgggct cgcgtagata gaagtgcagg cccgcgagcc gatgctggcc gtcgatgatc
     1681 aggaacggcg accgcggcat cgagaggtcg cccatctgcg cgtactgccc gagcttgctg
     1741 aacgcgagct cctcggacgt gaacagcagc accgccccgg gaacgaccgg ctggtcggcc
     1801 tcgttacgga agaagtcgcg gatctggcgc accttgcgcc ggttcagctc gcgctggaac
     1861 gccccggcgc ggcgctcgat cgcccgcaca tagcgctcca cgtcgtcgat ttcctcggcg
     1921 tccttgcccg aggcatcgcc cccgacgact tcgccgcggt cgccataaaa gcgggtctcg
     1981 aatcggacgt gcttgagcaa gtcttcggcc ggatagctca cgaaatagaa ctcggcgttc
     2041 ttctggcgca tccgcaatgc gagcatcgac ggtccccctg tgcgttgatg acaagaacga
     2101 tcgatgacgc cggcacggcc acgctcatcg ccgcgtgccg atccta
//
LOCUS       DASSNN010000149         3496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_645729, whole genome shotgun sequence.
ACCESSION   DASSNN010000149 DASSNN010000000
VERSION     DASSNN010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645729"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFL84_02610"
     CDS             <1..659
                     /locus_tag="VFL84_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607543.1"
                     /translation="AVFAPDDPLVLGALAANAARKQNLDESIALQTRAVALDPLSLVG
                     HVNLGCYFYYAGKFESAEAEWRAALDLAGASAPAANDLTRGLAVGLAQVELARQRSDS
                     ALALVDSWLAAGKERDYSLALIYGALGRTTDADVALARLREAGSRNEWETLKVIEAHA
                     FLGHIDAAFEWLAKAVDQSRQAYPRTPATMAWKTELRHSPFVTALREDPRWQAWLAEG
                     "
     gene            complement(607..2139)
                     /gene="lnt"
                     /locus_tag="VFL84_02615"
     CDS             complement(607..2139)
                     /gene="lnt"
                     /locus_tag="VFL84_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET7607544.1"
                     /translation="MTRTRAHYLLAIVAGAASPLAFAPFNQFWLAPLSYAALLYVWSG
                     ATPRRAFGLGLAYGCASFGLGTYWTYIAVRIIGQAPVFVGVTLTVGLVVVLAAFIAVA
                     GFAAARAFRTSGAVAWLATVPALFVLTEWLRGWLFTGFGWLAAGYSQTDSWLMGYAPL
                     GGIHLMSSAVLVTAGALLTLWLGSRRERAVAAIVIAAVWLGGLALRGRDFTEPKETPL
                     EVALATAAVPQVTKYDPAQLEPTLALYAELTRQGAGADLIIWPEAAIPIPIELVSGWL
                     ETVRRQAAERGSTLVLGALRTVPAAGGDATETYENVVLALTDEPQVYVKRHLVPFGEF
                     YPVPDFVRNWMRLMNLPYIDLAVGADDQPLLAVAGERLGVTICYEDVFGAEQLRHLAD
                     TTLLVNVSNDGWFGESIAIPQHLQIARVRAAEAGRYLVRAANRGITAVIDPHGRLVDT
                     IEPFHAGVLKATVRGYTGATPYARIGDWLVVVAAMAALLAVRAASLRRATPASADLRA
                     AR"
     gene            complement(2145..2999)
                     /locus_tag="VFL84_02620"
     CDS             complement(2145..2999)
                     /locus_tag="VFL84_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HET7607545.1"
                     /translation="MSDDTHPSSDTGVSGWWQRLVQGRVEAKTRDDIAEFLAGFLERG
                     VIGEDEFAMLEGVLKVADTQVRDIMVPRSHMVVVAKDQTPDEVLKVITESNHSRFPVI
                     GDDKDEIIGIVLAKDMLKHVIANQTPFDLQLFLRPAVFIPESKRLNTLLTEFREGRNH
                     MAIVVDEYGGVSGLATIEDVLEQIVGDIDDELDPEEASSIQPQDGGKYIVAALTRIDE
                     FNEFFNTELSDADYDTVGGLVMHELGRLPRRGEQLDFAGLRFKVLRGDRRRLHTLEVT
                     RLPPPGSE"
     gene            complement(3007..3483)
                     /gene="ybeY"
                     /locus_tag="VFL84_02625"
     CDS             complement(3007..3483)
                     /gene="ybeY"
                     /locus_tag="VFL84_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET7607546.1"
                     /translation="MTRRARAVAVTVQNAARGVAHPAAADLKRWALGALAPDVRGELT
                     IRIVAEHESAELNARYRGKAGATNVLSFGAQPVPAASGEELLPYGDVVICAAVVEREA
                     REQGKPAAAHWAHMVVHGALHLQGYDHENMRDAGIMEARERAVLAELGFPDPYSIK"
BASE COUNT          562 a         1220 c         1183 g          531 t
ORIGIN      
        1 cggccgtgtt cgcgcccgac gatccattgg tgctcggcgc gctcgccgcc aacgccgcgc
       61 gcaagcagaa cctcgacgag agcatcgcgc tgcaaacgcg cgccgtggcc ctcgatccgc
      121 tgtcgctcgt gggtcacgtg aacctcggct gctacttcta ttacgccggc aagttcgaat
      181 ccgccgaggc ggaatggcgc gcggctctcg atctggccgg agcttcggcg cccgccgcga
      241 acgacctgac gcgcggactt gcggtcggcc tcgcgcaagt cgagctcgca cggcagcgct
      301 ccgacagtgc gctcgcgctc gtcgactcct ggctcgcggc gggaaaagaa cgcgactatt
      361 cgctcgctct gatctatggc gcgctcggcc gcacgaccga tgccgacgtc gcgctcgcgc
      421 gcttacgcga agccggcagc cgtaacgagt gggaaaccct caaggtcatc gaggcgcacg
      481 cgttcctcgg gcacatcgac gcggctttcg agtggctcgc caaggcggtc gatcagagcc
      541 ggcaagccta tccgcgcacg cccgcgacga tggcatggaa gaccgagctc cgccattcgc
      601 cgttcgtcac cgcgctgcgc gaagatccgc gctggcaggc gtggctcgcc gaaggctagc
      661 cgcgcgcacc gcgagcagcg cggccatcgc cgccacgacg acgagccagt cgccgatgcg
      721 cgcatacggc gtcgcgcccg tgtagccgcg caccgtcgct ttcaacacgc cggcgtgaaa
      781 cggctcgatc gtgtcgacga gacggccgtg cggatcgatg acggccgtga tgccgcggtt
      841 ggccgcgcgc accaagtacc gccctgcttc cgccgcacgc acgcgcgcga tctgcaagtg
      901 ctgcgggatc gcgatcgatt cgccgaacca gccgtcgttg ctgacgttga cgagcagcgt
      961 cgtgtcggcg aggtgccgaa gctgctcggc gccgaagacg tcctcgtagc agatcgtgac
     1021 gccgagccgc tcgccggcca cggcgagcag cggctggtcg tcggccccga cggcgaggtc
     1081 gatgtacggc aggttcatga gccgcatcca attgcgaacg aaatcgggca cgggatagaa
     1141 ctcgccgaac ggcacgaggt ggcgcttgac gtagacctgc ggctcgtccg tcagcgcgag
     1201 caccacgttc tcgtaagtct ccgtggcatc gccgccggca gccggcacgg tgcgcagcgc
     1261 gccgagcacc agcgtgctgc cgcgctcggc cgcctgccgc cgcacggtct cgagccagcc
     1321 gctcacgagc tcgatcggaa tcgggatcgc ggcctccggc cagatgatga ggtcggcgcc
     1381 cgcgccttgg cgcgtgagct cggcgtagag cgcgagcgtc ggctcgagct gcgccggatc
     1441 gtacttcgtc acctgcggga ccgcggccgt cgcgagcgcg acttcgagcg gggtttcctt
     1501 cggctcggtg aaatcgcggc cgcgaagcgc gaggccgccg agccagactg ccgcgatcac
     1561 gatggcggcg acggcgcgct cgcgccggct gccgagccag agcgtgagca acgcccccgc
     1621 cgtcacgagg acggccgagc tcatcaggtg gatgccgccg agcggcgcgt agcccatgag
     1681 ccacgagtcg gtttggctat agccggccgc gagccaaccg aagcccgtga acagccagcc
     1741 gcgcagccac tccgtgagca cgaacagcgc cggcaccgtc gcgagccacg cgacggcgcc
     1801 gctcgtgcga aaggcgcgcg cggccgcgaa gccggccacc gcgatgaagg ccgcgagcac
     1861 gaccacgagc ccgaccgtca gcgtcacgcc gacgaacacg ggcgcctgac cgataatgcg
     1921 taccgcgatg taagtccagt acgtgccgag cccgaagctg gcgcagccgt aggcgagacc
     1981 caacccgaac gcgcgccgcg gcgtcgcgcc gctccagacg tagagcaacg ccgcgtagga
     2041 caacggcgcg agccagaact ggttgaacgg cgcgaacgcg agcggtgacg ccgcgcctgc
     2101 cacgatcgcg agcaggtagt gcgcgcgcgt gcgggtcaaa gcggctactc ggagcccggc
     2161 ggcggcagcc gcgtcacttc gagcgtgtgc aagcgccggc ggtcgccgcg cagcaccttg
     2221 aagcgtaagc ccgcgaagtc gagctgctcg ccgcggcgcg gcaagcgccc gagctcgtgc
     2281 atgacgaggc cgccgaccgt gtcgtagtcg gcgtcggaga gctcggtgtt gaagaactcg
     2341 ttgaactcgt cgatgcgcgt cagcgcggcg acgatgtact tgccgccgtc ctgcggctgg
     2401 atcgacgacg cttcctcggg atcgagctcg tcgtcgatgt cgccgacgat ctgctcgagc
     2461 acgtcctcga tcgtcgcaag gcccgacacg ccgccgtact cgtcgacgac gatggccatg
     2521 tggttgcggc cttcgcgaaa ttccgtgagc agcgtgttca ggcgcttgct ttccggaatg
     2581 aatacggcgg gacgcaagaa cagctgcaga tcgaacggcg tctgattcgc gatgacgtgc
     2641 ttgagcatat ctttcgcgag cacgatgccg atgatctcgt ccttgtcgtc gccgatgacg
     2701 gggaaacgcg agtggttcga ctccgtgatg accttcagca cctcgtccgg cgtttgatct
     2761 ttcgcgacga cgaccatgtg gctgcgcggg accatgatgt cgcgcacttg cgtgtccgcg
     2821 accttgagca cgccctcgag catcgcgaac tcgtcttcgc cgatgacgcc gcgctcgagg
     2881 aagccggcca agaactcggc gatgtcgtcg cgcgtcttcg cttcgacccg tccttgaacg
     2941 agccgctgcc accatccgga tacgccggtg tcgctactag ggtgagtatc gtccgacatt
     3001 ggaacgttat tttatcgaat agggatcggg aaagcccagt tcggcgagca ccgcccgttc
     3061 gcgggcttcc atgatacctg catcgcgcat gttttcgtga tcgtagccct gcaaatgaag
     3121 cgccccgtgc acgaccatgt gtgcccagtg cgccgcggca ggcttgcctt gctcgcgcgc
     3181 ctcgcgctcg acgacggcgg cgcagatcac gacgtcgccg tacggtaaca gctcttcgcc
     3241 gctcgccgcg ggaaccggct gcgccccgaa cgacaacacg ttcgtcgcgc cggccttgcc
     3301 gcgatagcgc gcgttgagct ccgcgctctc gtgctcggcg acgatacgga tcgtcagctc
     3361 gccgcgcacg tccggcgcga gcgctcccag ggcccagcgc ttcaggtcgg cggccgcggg
     3421 atgcgcgacg ccgcgcgcgg cgttctgcac cgtgaccgcg acggcgcgcg cgcggcgcgt
     3481 cattccgttt ccggtt
//
LOCUS       DASSNN010000150         4472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1417452, whole genome shotgun sequence.
ACCESSION   DASSNN010000150 DASSNN010000000
VERSION     DASSNN010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417452"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VFL84_02630"
     CDS             <1..1344
                     /locus_tag="VFL84_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7607547.1"
                     /translation="LAYMALFALSVGALSAFIYWATLGYLETQTNAIIEAEIRGLYEQ
                     YERSSIRGLSDVIQERVDRDTEGRMFYLLTDPIGRKVAGNVTAWPIGVDDARGRWVDF
                     VQRDSDTPVRGMWLPVGSFRLLVGRDIRELTAIRQLLRRASFYGISLTLVLALIGGVL
                     LALGAERRLAEINRTMRQIMAGDWSRRAPLKGTNDEHDELAQNINAMLDQIENLLEGM
                     RHVGDSVAHDLRGPITRLRNRLETLAAAEKPSREDLADSVAQLDQVLATFNALLRIAR
                     VESGAYRSAFTTVDLRPIVHDVCELYQAAAEESQVTLQARAAESVEVFGDRELLAQVL
                     TNLVDNAVKYTPPGGVVRIELARKGDLAQLRVADSGPGIPPEDRERVLQRFTRLDRAR
                     SQPGNGLGLALVNAVTLQHHGRLTLGDNGPGLVATVELPALAPPRTAVLATAAAR"
     gene            1489..>4472
                     /locus_tag="VFL84_02635"
     CDS             1489..>4472
                     /locus_tag="VFL84_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7607548.1"
                     /translation="MGTPRAFSAWMGMIAGCAAVLAGTHAFAQPIARDDSYSTPAGAP
                     LSIADPGVLTNDSAAAGGGLDANVVTLPAHGLLLLGPGGGFYYAPGNGFTGTDTFTYQ
                     AREAGTTLSNVATVTITVVAPNVPPVAADDAYATNEGQRLNVNSSNGVLANDTDANGN
                     PLTAALVTGVASGSLTLQANGSFEYTPAPGFAGPVTFTYQANDGTAASNTATVTITVN
                     AVDDPPVTQPESYTTAEDTPLTVAAPGVLGNDADPEGAPLTAELARDAPDSVLQLAPD
                     GSFSFLPDPDFNGTATFTYRARDGATQSAPTTVTITVTAVNDPPFAVNAAPRTVQEGA
                     TYTFTLIASDRDDTTPTITAPTLPRWLTFTSPATISGTPADADVGVHTVAMSITDGIA
                     PPVPVQWEITVEDVDHPPVIATIPEQTATEGTPVDIDLARFVTDSDTPVAELTYAAAA
                     APPPGLTLSTAGRLSGTPEIGAGVGTHTVRFTVSDGKTTVPGQLRLVVLAAGRVDLTV
                     TMNASPNPVTLDTPTTWTLTVTNRAPQVQAPGASLDVTFTGEVPFRFDAPAPTSGCTL
                     TPNGDQNRLTCTLGPLAGGASTTITLTGRSSLAGDVFAEAHVAVTGGALDETPGNDVG
                     AASLSIAQSVAGMPAQRIAVADARALAAGDLNADGFDDLAVATASAQGVVVFTNVADP
                     ANPGRRTFATPPLALGGEGLTNDIAIADLDRDNDLDIVTAAGNGAPNRAFLSAGGAFT
                     SASLGLADAESRAVAVGDVNGDAFVDLVFAGPGATTVLLGTGSGATFTAGGTVGPHDA
                     RDVALVNLLGDALPELVLANGDGDAAVYRNAGGAFTPELTLTTGSTSAVATGDFNSDG
                     RADLVFSRETAKLPAVPSSLVWLNTSSGQLVVSDELGAAITTRLLVRDFNLDSRSDVL
                     ALNGYGARIFTNAGSANGTLALHPQQLATPGARGVAAGKFSSDDRVDLAIVGDSLGVF
                     INDGSGNFGEPDSNAPVIQLRG"
BASE COUNT          724 a         1609 c         1492 g          647 t
ORIGIN      
        1 ctcgcctaca tggcgctgtt cgcgttgtcc gtcggcgcgc tatccgcgtt catctattgg
       61 gcgacgctcg gctatctcga gacgcaaacg aacgcgatca tcgaagccga gatccgcggc
      121 ctctacgagc aatacgagcg cagcagcatc cgcggcttga gcgacgtcat tcaagaacgc
      181 gtcgaccggg ataccgaggg gcgcatgttc tacttgctga ccgatccgat cgggcggaag
      241 gtcgccggta atgtgacggc atggccgatc ggcgtcgacg atgcgcgcgg ccggtgggtc
      301 gatttcgtgc agagggacag cgataccccg gtgcgcggca tgtggctgcc ggtcggcagt
      361 ttccggctgc tcgtcggccg cgacatccgc gagctgacgg cgatccgcca gctgctgcgg
      421 cgcgcgtcgt tctacggcat ctctctcacg ctcgtgctgg cgttgatcgg cggcgtgctg
      481 ctcgcgctcg gcgcggagcg ccgcctcgcc gagatcaatc gcacgatgcg ccagatcatg
      541 gccggcgact ggagcagacg cgcgccgctc aaggggacaa acgacgagca cgacgagctc
      601 gcgcagaaca tcaacgcgat gctcgaccag atcgagaatt tgctcgaagg catgcggcac
      661 gtcggcgaca gcgtcgcgca cgacctgcgc gggccgatca cgcggctgcg caatcggctc
      721 gagacgctcg ccgcggccga gaagccgagc cgtgaagatc tggcggactc cgtcgcgcag
      781 ctcgaccagg tgctcgcgac gttcaacgcg ctgctgcgca ttgcgcgcgt cgagtcgggc
      841 gcctatcgca gcgcgttcac gaccgtcgac ttgaggccga tcgtgcacga cgtgtgcgag
      901 ctctatcagg cggccgccga agagagtcaa gtgacgttgc aggccagagc cgccgagtcc
      961 gtcgaggtgt tcggcgatcg cgagctgctc gcgcaagtgc tcacgaatct cgtcgacaac
     1021 gccgtcaagt acacgcctcc gggcggcgtc gttcgcatcg agcttgcccg caagggcgat
     1081 ctcgcgcagc tacgcgtcgc cgacagcggc cccgggattc cgcccgaaga tcgcgagcga
     1141 gtgctgcagc gcttcacgcg cctcgatcgc gcgcgctcgc aacccggcaa cgggctcggc
     1201 ctcgcgctcg tcaacgccgt gacgctgcag catcacggcc gcctcacgct cggcgacaac
     1261 ggccccgggc tcgtcgcgac cgtcgagttg ccggcgctcg cgccgccgcg cacggcggtg
     1321 ctcgcgaccg cggcggctcg ctgaaaatct cgcgcttcgt caccgtcttt gggagcagcg
     1381 ctgcaaaaac tgcacggctg atcccgcgcg agaggcgccg agccgcttcc ttcgacggcc
     1441 cggcataaaa cctgcatccc ctggcgttgc cagggaggta ctgggccgat ggggacgccg
     1501 cgcgcgtttt ccgcatggat ggggatgatc gcaggatgcg cggcggtgct cgccggaacg
     1561 catgcgttcg cgcagccgat cgcccgcgac gattcctact cgactcccgc gggagcgccg
     1621 ctctcgatcg ccgaccccgg cgtcctgacc aacgactcgg ccgccgccgg cggcggtctc
     1681 gacgccaatg tcgtcacgct ccccgcgcat ggcctgctac tgctcggccc cgggggcggg
     1741 ttctactacg cgccgggcaa cggcttcacg ggcaccgaca cttttaccta tcaagctcgc
     1801 gaagccggca cgacgctgag caacgtcgcg acggtgacga tcacggtcgt ggcgcccaac
     1861 gtgccgccgg tagccgccga cgacgcctac gccacgaacg aaggacagag gctcaacgtc
     1921 aattcgagca acggcgtgct cgccaacgac accgacgcga acggcaatcc gttgacggcc
     1981 gctttggtca ccggcgtggc gagcggatca ctgacgcttc aggcgaacgg ctcgttcgag
     2041 tacacgccgg ccccagggtt tgcgggcccg gtcacgttca cctatcaagc caacgacggc
     2101 acggcggcta gcaatacggc gacggtcacg atcacggtca acgccgtcga cgacccgccg
     2161 gtcacacagc ccgagagcta cacgacggcg gaggacacgc cgctcaccgt cgccgcgccc
     2221 ggcgtcctcg gcaacgacgc cgatcccgaa ggcgcgccgc tgacggccga gctcgctcgc
     2281 gacgcgccgg acagcgtgtt gcagctcgcg cccgacggct cgttcagttt cttgccggat
     2341 ccggacttca acggcaccgc gacattcacc tatcgcgcca gagacggagc cacgcagagc
     2401 gcgccgacga cggtcacgat cacggttacg gccgtcaacg atccgccgtt cgcggtgaac
     2461 gcggcgccaa gaacggtgca ggaaggcgcc acttacacct tcacgctcat tgcgtccgat
     2521 cgcgacgaca cgacgcctac gatcacggca ccgacacttc caaggtggct gaccttcacc
     2581 tcacccgcga cgatctccgg cacgcccgcc gatgccgacg tcggcgttca caccgtggcg
     2641 atgagcatca cggacggcat cgcgccgccc gtcccggttc agtgggagat cacggtcgag
     2701 gacgtcgacc atccgccggt gatcgcgacg attccggagc aaacggcgac cgagggcacg
     2761 ccggtcgaca tcgacctcgc gcgcttcgtc accgactccg atacgccggt ggccgagctg
     2821 acttacgcgg cggcggccgc cccgccgcct ggcctgacgc tgagcaccgc cggacgcttg
     2881 tccggaacgc cggagatcgg cgccggcgtc ggcacgcaca ccgtgcgctt cacggtttcc
     2941 gacggcaaga ccacggtgcc gggccaactg cggctcgtcg tactggccgc gggccgagtc
     3001 gacctcacgg tcacgatgaa cgcgtcgccg aatccggtca cgctcgacac gccgacgacg
     3061 tggacgctga ccgtcacgaa ccgcgcgccg caagtccaag cgccgggagc atcgctcgac
     3121 gtcacgttca cgggcgaagt gccgttccgc ttcgacgcgc cggcgccgac gtccggttgc
     3181 acgctcacgc cgaacggcga ccaaaaccgt ttgacttgca cgctcggccc gttggccggc
     3241 ggcgcgtcga cgacgatcac gctgaccggc cgcagcagct tggcgggcga cgtgttcgcc
     3301 gaggcgcacg tcgcagtcac gggcggcgcg ctcgacgaga cgcccggcaa cgacgtcggt
     3361 gctgcatcgc tgagcatcgc gcagagcgtc gccgggatgc cggcgcagcg tattgcggtc
     3421 gcggacgcgc gcgcgctggc ggcaggcgac ctcaatgccg acggcttcga cgatctcgcc
     3481 gtcgcgacgg catcggcgca aggcgtcgtc gtgttcacga acgtcgcgga tccggccaac
     3541 cccggccggc gcacgttcgc aacgccgcct ctggccctcg gcggcgaggg actgaccaac
     3601 gatatcgcga tcgcggatct cgatcgcgac aacgacctcg acatcgtgac ggccgccggc
     3661 aacggcgcgc cgaaccgcgc gttcctatcg gcgggcggag cgttcacgag cgcgtcactc
     3721 gggctagccg acgccgagag ccgcgccgtc gcggtcggcg acgtcaacgg cgatgcgttc
     3781 gtcgacctgg tgttcgccgg ccccggcgcg acgaccgtgc tgctcggtac gggctcgggc
     3841 gcgacgttca cggccggcgg aaccgtgggg ccgcacgacg ctcgcgacgt ggcgctcgtc
     3901 aacctgctcg gcgacgcgct acccgagctc gtgctcgcga acggcgatgg cgacgcagcg
     3961 gtgtatcgaa acgccggcgg cgcgttcacg ccggagctta ccctcacgac gggctcgacg
     4021 agcgccgtcg cgacgggcga cttcaactcc gacggccggg cggacctcgt cttctcgcgc
     4081 gaaacggcga agctgcctgc ggtgccgagc tcgctcgtct ggctcaacac gtcgagcggc
     4141 cagctggtcg tctcggacga gctcggcgcg gcgatcacga cgcgcttgct cgtgcgcgac
     4201 ttcaatctcg actcgcgctc cgacgtgctc gccttgaacg gctacggcgc gcggatcttc
     4261 acgaatgcgg gctcggccaa cggcacgctg gcgctgcacc cgcaacagct cgccacgccc
     4321 ggcgcgcgcg gcgtcgcggc cggcaaattc agcagcgacg accgcgtaga tctcgccatc
     4381 gtaggcgact cgttgggcgt gttcatcaac gacggctccg ggaatttcgg cgagcccgac
     4441 tcgaacgcgc ccgtcatcca gctgcgcggc ga
//
LOCUS       DASSNN010000151         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_31174, whole genome shotgun sequence.
ACCESSION   DASSNN010000151 DASSNN010000000
VERSION     DASSNN010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31174"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /gene="ruvA"
                     /locus_tag="VFL84_02640"
     CDS             <1..518
                     /gene="ruvA"
                     /locus_tag="VFL84_02640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET7607549.1"
                     /translation="EAPMSTFYRLPDVGQALTLVTHLVIREDAHVLYGFSTESERTLF
                     RSLLKVGGVGPRMALAVLSGMSVDGFYDCIRGKDMASLTRIPGIGRKTAERLLLEMAD
                     RLPAEILTFPEGRRAAATPESEAQDALLALGYKAAEVEKMLKDLDSGRLSTEELIREA
                     LRRVHSRGGRA"
     gene            515..1555
                     /gene="ruvB"
                     /locus_tag="VFL84_02645"
     CDS             515..1555
                     /gene="ruvB"
                     /locus_tag="VFL84_02645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET7607550.1"
                     /translation="MNGEARLIAAGAAAEDEAFDRALRPRRLEDYVGQPRVKEQMEIF
                     ITAARRRGEALDHTLLFGPPGLGKTTLAHIVANELGVTLHQTSGPVLERPGDLAALLT
                     NLEPRDVLFVDEIHRLSPVVEEVLYPALEDFQLDIMIGEGPGARSIKLDLPPFTLVGA
                     TTRAGLLTSPLRDRFGITQHLEFYGQAELARIAARTAGILNLPIDEAGAAEIAARARG
                     TPRIAIRLVRRVRDYAEVKADGRVDRDVARAAMDLLEVDPHGFDAMDRRLLLAIIEKF
                     DGGPVGVESLAAAIGEERGTLEDVVEPYLIQEGFLQRTARGRIATKSAYRLFGLKLPE
                     GRESPDTPDLFS"
     gene            1552..1986
                     /locus_tag="VFL84_02650"
     CDS             1552..1986
                     /locus_tag="VFL84_02650"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HET7607551.1"
                     /translation="MSGAAAAAFVLPVRVYLEDTDAQGVVYNASYFRFMERARTEWLR
                     ARGLEHAQLRDEHGVQLVLTRVEAELRAPARLSDLLLVEAAALRARGARIVFAHEIYR
                     QGPGERGAELVCRGVAEVGCIDAELGRPRRLPASLLAAIQGS"
     gene            2032..>2503
                     /locus_tag="VFL84_02655"
     CDS             2032..>2503
                     /locus_tag="VFL84_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7607552.1"
                     /translation="MNTDLSILQLVLDASPVVKAVLALLLAASIASWAVIIEKQRVVK
                     RAMRGADEFENEFWSGGDLGSLYREVSQSDEKPVGMAGIFESGFREFGRLRQEGGLPP
                     EQLVVGARRSMHVSQLREVDRLEHNLGFLATVSNTSPYIGLFGTVWGIMSSFHAL"
BASE COUNT          402 a          788 c          884 g          429 t
ORIGIN      
        1 tcgaggcgcc gatgtccacg ttctaccgtc tgcccgacgt cggccaagcg ttgactctgg
       61 tgacgcacct cgtgattcgc gaggatgccc acgtgcttta cggtttctcg acggagagcg
      121 agcgcactct gttccgcagc ctgctcaagg tcggcggcgt aggcccgcgc atggcgctgg
      181 ccgtgctctc gggcatgagc gtcgacggtt tctacgactg catacgcggc aaggacatgg
      241 cgagtctcac gcgcattccg ggcatcggcc gcaagacggc ggagcggctg ctgctcgaga
      301 tggccgatcg gctgcccgcg gagattctga ctttccccga aggccggcgg gctgcggcga
      361 cgcccgagag cgaagcccag gatgcgctgc tcgcgctcgg ctacaaggcg gccgaggtcg
      421 agaagatgct caaggatctc gactcggggc gcctttcgac tgaagagctc atccgcgagg
      481 cgctgcggcg cgtgcattcg cgaggagggc gcgcatgaac ggcgaggcgc ggctcatcgc
      541 cgccggggcg gcggccgaag acgaggcgtt cgaccgcgcg cttcgcccgc ggcggctcga
      601 ggactacgtg gggcagccgc gagtcaagga gcagatggag atcttcatca cggcggcgcg
      661 gcggcgcggg gaagcgttgg atcacacgct gctgttcggc cccccgggtc tcggcaagac
      721 cactttggcg cacatcgtcg cgaatgagct cggcgtgacc ctgcatcaga cttcgggacc
      781 ggtgctcgag cggccaggcg atctcgccgc gctgctcacg aacctcgagc cgagagatgt
      841 tttgttcgtc gatgaaattc accgcctgag cccggtcgtc gaagaggtgc tgtatcccgc
      901 gctcgaggat ttccagctcg acatcatgat cggagaaggg ccgggcgcgc gctccatcaa
      961 gctcgacttg ccgcctttca cgctcgtcgg cgccacgact cgggcgggcc ttctgacctc
     1021 gcctttgcgc gatcgcttcg gcattacgca gcacctcgag ttttacggcc aggcggagct
     1081 cgcgcgcatc gcggcgcgca cggccggcat cttgaacctg cccatcgacg aggccggcgc
     1141 ggccgagatc gccgctcgcg cacgcggcac gccgcgcatc gcgatccgtc tcgtgcgccg
     1201 ggttcgcgat tacgccgagg tcaaggcgga cggacgcgtg gaccgcgacg tcgcgcgtgc
     1261 ggccatggat ctgctcgagg tcgatccgca cggcttcgac gcgatggacc gcaggctgct
     1321 gctcgcgatc atcgagaagt tcgacggcgg accggtcggc gtcgagagtc tcgccgcggc
     1381 catcggcgag gaacgcggca cgctcgaaga cgtggtggag ccgtatttga ttcaggaggg
     1441 ctttctgcag cgcacggcgc gcggccgaat cgcgaccaaa tccgcctatc ggctgttcgg
     1501 cttgaaactg cccgaaggac gcgagagccc ggacacgccg gatctgttct catgagcggc
     1561 gcggcagcgg cggctttcgt gctgcctgtg agggtgtatc tcgaggacac ggacgcacaa
     1621 ggtgtcgttt acaacgctag ctattttcgc ttcatggagc gggcgcgcac cgaatggctc
     1681 agggcacgag ggctcgagca cgcgcagctg cgcgacgagc atggagtgca gctcgtgttg
     1741 acgcgcgtcg aggccgagct ccgggcgccc gcgcgcctca gcgatttgct gcttgtcgag
     1801 gccgcggcgc tgcgcgcacg cggggcgcgc atagtgtttg cgcacgagat ttaccggcaa
     1861 gggccgggag agcgcggggc ggagctcgtc tgccgcggcg ttgccgaggt cggctgcatc
     1921 gacgccgagc tcggcaggcc gcgccggctg ccggcctcgc ttttggcggc gatacaaggg
     1981 agctgaataa ggaatgaacg ccgcggcaac actcaagagt gggttgaagg aatgaacacc
     2041 gatttatcga tactgcagct cgtgctcgac gcaagccccg tcgtcaaggc cgtgttggcc
     2101 ctgctgctcg ccgcgtcgat cgcgtcctgg gccgtcatca tcgagaagca gcgcgtcgtt
     2161 aaacgcgcga tgcgcggcgc cgacgagttc gagaacgaat tctggtcggg cggagatctt
     2221 ggcagtctgt accgcgaggt gtcgcaaagc gacgagaaac cggtgggcat ggccgggatc
     2281 ttcgagtcag gcttcaggga gttcggccgg ctcaggcagg agggcggctt gccgccggag
     2341 cagctcgtcg tgggcgcccg gcgctcgatg cacgtttccc agctgcgcga agtcgacagg
     2401 ctcgagcaca atctcggctt tctcgcgacg gtcagcaata cgagtcctta catcgggctt
     2461 ttcggcacgg tttggggcat catgagctcc tttcatgcgc tcg
//
LOCUS       DASSNN010000152         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1655055, whole genome shotgun sequence.
ACCESSION   DASSNN010000152 DASSNN010000000
VERSION     DASSNN010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655055"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2208
                     /gene="glnE"
                     /locus_tag="VFL84_02660"
     CDS             <1..2208
                     /gene="glnE"
                     /locus_tag="VFL84_02660"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HET7607553.1"
                     /translation="LLDQPTEDGIAYRVDTRLRPFGTSGPLVVSVPALETYLVQHGRD
                     WERYAYVKARLLTGVHFASDIFDYVLTPFVYRRYLDYGVFDALRQMKRLIAQEVARKD
                     LAENIKLGPGGIREIEFIVQAFQIVRGGRRPELRARSLLTVLPLLAGDRQLPPSTVAA
                     LAAAYRYLRTVENYIQAMNDAQTHELPADEEERERLAYALHEPSWAAFHERLRRERAV
                     VEAEFQRVAWEAEGAGGRELDPLFAAWASGDVAAMLKGTALAGDERALDLLKDLRHGG
                     LYQRMDEVGRQRLAAVMVRTLRAIAQSPAAPKCFERVLPVYRAVARRSAYLALLNENP
                     ASLDRLLKLVTDSSLLARQIAEQPMLLDELLDARVFEAPPSREELAALLERATQGISE
                     SDLEEMLDAIRVFQRTAIFRIAVADRLGSLPLMKVSDRLTDTAELVLDYSLRAAWREL
                     VAKHGKPRCGPPPRDAGFAVIGYGKLAGLELGYGSDLDLVFLHDSSGAQQETDGVPPL
                     DNERFFSRLVQRLIHFLSVQTSSGRLYDVDTRLRPSGRSGLLVTSLEGFRHYQTHDAW
                     VWEHQALLRSRALAGSAAVCRAFEAIRRDVLVSHVDRAKLKAEIAKMRRRMREELSLA
                     EAGSFDIKQDEGGIADVEFLVDYWVLAHSGDYPELVEFPDNIRQLEGLARVGLVPEEL
                     CLRLKDAYLTLRRRVHELALDEGGRVVPEPELRAVRDFVSGVWRDVFEDAERE"
     gene            2258..2479
                     /locus_tag="VFL84_02665"
     CDS             2258..2479
                     /locus_tag="VFL84_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019557721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HET7607554.1"
                     /translation="MRPRHGSVQEHLIAANAERVYQVTRADLPLHCPTPSMYLWNSHP
                     KVYLPIEVTGEAKCPYCSSTYRLVEDDAR"
BASE COUNT          391 a          852 c          828 g          426 t
ORIGIN      
        1 ctgctcgatc agccgaccga ggacggcatc gcgtaccgcg ttgacacgcg gctgcggccg
       61 ttcggcacga gcggaccgct cgtcgtcagc gtgccggcgc tcgaaacgta tctcgtccag
      121 cacggccgcg actgggagcg gtacgcgtac gtcaaggcgc gcttgctcac aggcgtgcac
      181 ttcgcttccg acatcttcga ttacgtcctc acgccattcg tctatcgccg ctacctcgac
      241 tacggtgtgt tcgacgcgct gcggcaaatg aagcgcctga tcgcgcagga agtcgcgcgc
      301 aaggatctcg ccgagaacat caagctcggc cccggcggca tccgcgagat cgagttcatc
      361 gtgcaggcgt ttcagatcgt gcgcggcggc cgccggcccg agctgcgcgc tcgctcgttg
      421 ctaactgttt tgccgctctt ggccggcgac cggcagctgc ccccgagcac cgtcgccgcg
      481 ctcgccgccg cgtatcgcta tctgcgcacc gtcgagaact acattcaggc gatgaacgac
      541 gcgcagacgc acgagctgcc tgccgacgag gaagaacgcg agcggctcgc ctatgcgctg
      601 cacgagccga gctgggccgc gttccacgag cgtctgcgcc gtgagcgcgc ggtcgtcgaa
      661 gccgagttcc agcgcgtcgc gtgggaggcc gaaggcgcgg gaggcagaga gctcgatccg
      721 ctcttcgccg cctgggcctc gggcgacgtc gccgcgatgc tcaagggcac tgcgctcgca
      781 ggcgacgagc gcgcgctcga tctcttgaag gatctacggc acggcggtct ataccagcgc
      841 atggacgagg tcggccggca gcggttggcg gccgtcatgg tgcgaacgct gcgcgcgatc
      901 gcgcagagcc cggccgcgcc gaagtgcttc gagcgcgtgc tgcccgtgta tcgcgccgtt
      961 gcacggcgca gcgcctatct cgctctgctg aacgagaatc ccgcttcact cgatcggctg
     1021 ctcaagctcg tcaccgacag ctcgttgctc gcgcgccaga ttgccgagca gccgatgctg
     1081 ctcgatgagc tgctcgacgc gcgcgtgttc gaggcgccgc cgtcgcgcga ggagcttgcc
     1141 gcgctgctcg agcgcgcgac gcaaggcatc tccgagagcg acctcgaaga gatgctcgac
     1201 gcgatccgcg tctttcagcg cacggcgata ttccgcatcg cggtagccga ccggctcgga
     1261 tcgttgccgc tcatgaaggt cagcgaccgg ctcaccgaca cggccgagct cgtgctcgat
     1321 tactcgttgc gtgccgcctg gcgcgagctc gtcgcgaagc acggcaagcc gcgttgcggt
     1381 ccgccgccgc gcgatgcggg gttcgccgtc atcggctacg gcaagctcgc cggcctcgag
     1441 ctcggctacg gctcggatct cgatctcgtc ttcttgcacg attcgagcgg cgcgcagcag
     1501 gagaccgatg gcgtgccgcc gctcgacaac gagcgcttct tcagccgact cgtgcagcgg
     1561 ctcatccact ttctttccgt tcaaacgagc tcgggccgtt tgtacgacgt cgacacgcgc
     1621 ctccggccga gcggccgatc gggcttgctc gtcacgagcc tcgagggctt tcgccattac
     1681 cagacgcacg acgcgtgggt gtgggagcac caggctctgc tacgcagccg cgccctcgcc
     1741 gggtcggccg cagtctgccg cgctttcgag gcgattcggc gcgacgtgct cgtctcgcac
     1801 gtcgatcgcg ccaagctcaa agccgaaatc gcgaagatgc ggcggaggat gcgcgaagag
     1861 ctgtcgctcg ccgaggccgg ctcgttcgac atcaagcagg acgagggcgg catcgccgac
     1921 gtcgagttcc tcgtcgacta ctgggttctc gcgcactcgg gcgactaccc ggagctcgtc
     1981 gagttcccgg acaacatccg gcagctcgag ggcctcgcgc gagtcgggct ggttccggaa
     2041 gagctgtgcc tccgcttgaa ggacgcctat ctcacgttgc gccggcgcgt gcatgagctc
     2101 gcgctcgacg aaggcgggcg cgtcgtgccg gagcccgagc ttcgagccgt ccgcgacttc
     2161 gtcagcggcg tttggcgcga cgtattcgaa gatgccgagc gagaataggg cgctgccgcg
     2221 ggtataatcg cggcctcgtc gggcaggaga acgagcggtg cgaccacgtc acggcagtgt
     2281 ccaggagcat ttgatcgcgg cgaacgccga gcgtgtctat caggtcacgc gggccgacct
     2341 gccgctccat tgccctaccc caagcatgta tctgtggaac tcccacccga aggtctattt
     2401 acccatagag gtcacgggcg aagcgaaatg cccttattgc agctcgacgt atcgcttggt
     2461 cgaggacgac gctcgctgac ccgccgactc gcgccat
//
LOCUS       DASSNN010000153         3009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1544013, whole genome shotgun sequence.
ACCESSION   DASSNN010000153 DASSNN010000000
VERSION     DASSNN010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544013"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFL84_02670"
     CDS             complement(<1..207)
                     /locus_tag="VFL84_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET7607555.1"
                     /translation="MRIGFTYDLKDDYLALGLAEHEVAEFDSGSTIDAIAGALTELGH
                     EVERVGHVRALAARLVAGWRCDLVF"
     gene            complement(242..1339)
                     /locus_tag="VFL84_02675"
     CDS             complement(242..1339)
                     /locus_tag="VFL84_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HET7607556.1"
                     /translation="MKAAEWNDWRWQNRHRIRTLKQLEGMVAVSDEERAAIQRHEGPL
                     PIGVTPYYMSLIDPADPMQGLRRTTIPTLAEFDRTPGEQDDPLGEDAHSPVPGLVHRY
                     PDRVLLLVTNFCSVYCRYCTRARLVGASGERALRKADIDRAIEYIAATPAVRDCLISG
                     GDPLSLDEERLEYVLSRLKSIPHLEFIRIGSKQPVVQPMRVTPTLTKMLRRYHPLWMS
                     LHFTHPDEITPEVAEACGRLADAGIPLGSQTVLLKGVNDDLTTLKKLFHALLKIRVRP
                     YYLYQCDPIRGSSHFRTTVDKGLELIGQMRGYTTGYAVPNYVVDAPGGGGKIAMLPGA
                     VIGRDGDNLLLRNFAGEQCRYPDPDGTLGKP"
     gene            1783..>3009
                     /locus_tag="VFL84_02680"
     CDS             1783..>3009
                     /locus_tag="VFL84_02680"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET7607557.1"
                     /translation="MTKNATLTYENKTIELPTVVGTEAEVAVDISQLRAKSGLITLDS
                     GYANTGACMSNITFIDGEQGILRYRGYPVEELAERSTFVETAYLLIFGELPTATQLAD
                     FRRLLGQHEFIHEDMLHHFDGFPRNAPPMAILSAMINALGCFQPEIMQPEENEHFMQA
                     AARLMSKVRTIAAASYRKSIGRPVNYPRTELRYTANFLHMMFSLPHKLYQPDEVVVRA
                     LDQLLLLHADHEQNCSTSTVRMVGSSQANLHASIAAGVCALWGPLHGGANVAVIEMLT
                     ELKNSGEKLETFIARVKDKDAHARLMGFGHRVYKNFDPRARIIKESCYALLKKLNVND
                     PLLDLAMRLEQVALNDEYFVKRKLYPNVDYYSGIIMRALGIPTNMFTVMFAIGRLPGW
                     IAHWREVRISGTQRIDR"
BASE COUNT          535 a          985 c          980 g          509 t
ORIGIN      
        1 gaacacgagg tcgcagcgcc accccgcgac gagccgcgcc gcgagcgcgc ggacatggcc
       61 gacgcgctcg acctcgtgac cgagctcggt caacgcgccc gcgatcgcgt cgatcgtcga
      121 gcccgagtcg aactcggcga cttcgtgctc ggcgagcccg agcgccaagt agtcgtcctt
      181 caggtcgtag gtgaacccga tgcgcacgtc ggtctcttgc ggcgcggaac gcgccaaaag
      241 gttacggctt gccgagtgta ccgtcgggat ccggataccg gcactgctcg cccgcgaagt
      301 tgcgcagcag caggttgtcg ccgtcgcggc cgatgacggc gccgggcagc atcgcgatct
      361 tgccgccgcc gcccggcgca tcgacgacgt agttcggcac cgcgtatccc gtcgtgtagc
      421 cgcgcatctg gccgatcagc tcgaggcctt tgtcgacggt cgtgcggaag tggctcgagc
      481 cgcggatcgg gtcgcactga tagaggtaat acggacgcac gcggatcttg agcagcgcgt
      541 ggaacagctt cttcagcgtc gtaaggtcgt cgttgacgcc cttcaagagc acggtctggc
      601 tgccgagcgg aatgccggcg tcggccaacc gcccgcacgc ttcggcgacc tcgggagtga
      661 tctcgtccgg gtgcgtgaag tgcaggctca tccagagcgg gtgatagcgg cgcagcatct
      721 tcgtcaacgt cggcgtcacg cgcatgggct ggacgacggg ctgcttgctg ccgatccgga
      781 tgaactcgag atgcggaatc gacttgaggc gcgacagcac gtactcgagg cgctcctcgt
      841 cgagcgaaag cggatcgccg cccgagatta ggcagtccct gaccgccggc gtggccgcga
      901 tgtattcgat ggctcggtcg atgtcggcct tgcgcagcgc gcgctcgccg ctcgcgccga
      961 cgagccgggc ccgcgtgcaa taacggcagt agaccgagca gaagttcgtg acgagcagca
     1021 gcacgcgatc cgggtagcgg tgaacgaggc ccggcaccgg actgtgggcg tcctcgccga
     1081 gcgggtcgtc ctgctcgcct ggcgtgcgat cgaactcggc gagcgtcggg atcgtcgtac
     1141 ggcgcaggcc ctgcatcgga tcggccggat cgatgaggct catgtagtac ggcgtcacgc
     1201 cgatcggcag cggaccttcg tggcgctgga tcgcggcgcg ctcctcgtcg ctgacggcga
     1261 ccatgccctc gagctgcttc agcgtgcgga tacggtgacg gttctgccaa cgccagtcgt
     1321 tccattcggc ggccttcacg ccggggaagt gacgtttgcg aaattcgttc gcggcggcgc
     1381 tgacgacgaa acgcgcgcga gcggccggcg cgagttggac cggtgcgccg ttgccgttcg
     1441 ctggggtttc aggttgcttg agagaggact ttcggaggga cagttcggct tgcgccaaac
     1501 tcggaggctc ttcctccagg gacagcagct tgctcaccgg tcgtccaagg gctcggagcc
     1561 catcgtctgt tcgttacccg gcaattccgt cgttacctgg gtgggcagac cttctacccg
     1621 caacgaaaac ggccgtaaat ataaatcgag gctcatagat tgcaagcatt ttctgtgcgg
     1681 atttgcgacg ctcgtcgagt gccgcgcaga cggcgcggac cgtgccgcca tcacacttaa
     1741 cgcgggaggc cagcgtagaa tacgccgtca gtcggaggcc ccatgacgaa gaacgcgact
     1801 ctcacgtacg agaacaagac catcgaattg cccacggtcg tcggcacgga agccgaagtc
     1861 gccgtcgaca tctcgcagct gcgcgccaaa tcggggctga tcacgctcga cagcggctac
     1921 gccaacaccg gcgcgtgcat gagcaacatc acgttcatcg acggcgagca ggggattctc
     1981 aggtatcgcg gctacccggt cgaggagctg gcggagcggt cgaccttcgt ggagacggcc
     2041 tatctcttga tcttcggcga gctgccgacg gcgactcagc tcgccgattt ccggcgcttg
     2101 ctcgggcagc acgagttcat ccacgaggac atgctgcacc acttcgacgg cttcccgagg
     2161 aacgcgccgc cgatggcgat cctgtccgcg atgatcaacg cgctcggctg ctttcagccg
     2221 gagatcatgc agccagagga aaacgagcac ttcatgcagg ccgccgctcg gctcatgagc
     2281 aaggtccgga cgatcgcggc ggccagctac cgcaagtcga tcggccggcc cgtcaattac
     2341 ccgcgcaccg agctgcgcta tacggccaat ttccttcaca tgatgttctc gctgccgcac
     2401 aagctctatc agcccgacga ggtcgtcgtg cgcgcgctcg atcagctgct gctgctgcac
     2461 gccgaccacg agcagaactg ctcgacctcg acggtccgca tggtcggctc gtcgcaggcg
     2521 aacctgcacg cgtccatcgc cgccggcgtc tgcgcgctct ggggcccctt gcacggcggc
     2581 gcgaacgtgg ccgtcatcga aatgctgacc gagctcaaga acagcggcga gaagctcgaa
     2641 acgttcatcg cccgggtcaa ggacaaagac gcgcacgcgc gcctcatggg cttcggtcac
     2701 cgcgtctaca agaacttcga cccgcgcgcg cgaatcatca aagaatcgtg ctacgcgctg
     2761 ctgaagaagc tgaacgtcaa cgacccgctg ctcgacctcg cgatgcggct cgaacaggtc
     2821 gcgctcaacg acgagtactt cgtcaagcgc aagctctatc cgaacgtcga ctattacagc
     2881 ggcatcatca tgcgggcgct cggcatcccg acgaacatgt tcaccgtcat gttcgcgatc
     2941 gggcgcttgc ccggctggat cgcgcactgg cgcgaggtcc gcatcagcgg cacccagcgc
     3001 atcgaccgg
//
LOCUS       DASSNN010000154         3350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1039672, whole genome shotgun sequence.
ACCESSION   DASSNN010000154 DASSNN010000000
VERSION     DASSNN010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039672"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VFL84_02685"
     CDS             <1..785
                     /locus_tag="VFL84_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607558.1"
                     /translation="VIDMQSLKAIKTIEGLHEPLNITFTPDGQKAYIGNYGAHWVGVV
                     DVTRHELIKKINVDTVPGVARLDPAKYLTEIKGIFNATPTPDGRFLYAADGDLGVVGV
                     IDTREDKVVKVIRVGPDPWRLYMSHDGKYGITANNGDSTISIIDIAKNAVVATYPVGP
                     DLTGINFAAGKAFVISSTTGFLYVFDMATLKPAGRIKIGNNLFLQTATTDTADQKLYL
                     GSATDNSVYIIDAATEAVERVANVGQFPWGTHIMDSKDNYCH"
     gene            782..1549
                     /locus_tag="VFL84_02690"
     CDS             782..1549
                     /locus_tag="VFL84_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607559.1"
                     /translation="MMIATAAPLRLVGRLCGVVLLGIAAGIFIFRSALAQEPQPEPPD
                     SPPDSAAAPAEGGVRARTRLVRRSATAVTDEQASELTLTLTVVSFRPIQTWVRTAGVL
                     DAAGKVLTAQLRSPEAELVEVGQRVRTFSVNTRTQMVQAKITRVTPRPGGATVEATLA
                     AAVANDGARHLMEIVTEKGPLLSVPNESIIEEAYAQVVYLKLPSGEFAPRTIETGITG
                     ELYTEIVDGLEDGDEVVSIGSFFVDADNKLKGGPAEP"
     gene            1546..>3350
                     /locus_tag="VFL84_02695"
     CDS             1546..>3350
                     /locus_tag="VFL84_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7607560.1"
                     /translation="MNPALFEGIVRARWLIALLILAGIVASVFAMRAAPLDAIPDTSD
                     PQVIVYAKWARSPELLEEQVTEPIVKALIGSPDVRSIRATSHMGYSFIYVILKSTAER
                     AAVRQLIADRLATIRQLLPADATVSIGPNASGIGWIYQYALIDHEKLRDPREMRLLNE
                     TQLKPALQAVPGVAEVASVGGLEKQYQLKLFPPLLADAGISLRELTVALRDALEEAGG
                     RVLEVTNRDYQIRGVVNHDSIDDLEALIVGHAPDGRAIRVKDIGYIQIGYDQRRGIAD
                     LNGEGEVVGGIVIMEQSQNALEITAALKKRLAEVAATLPQGIEIATTYDRSQLIWGTL
                     RHFFITLAYELGVVILVTALFLRNARTSLAPVSILLLGVLFTAIPMALLNQTINLFSL
                     AGLFIAIGEMVDATIVIVENSTAELAAHPNASETEKRQIVLRSIAGVAKPLLFSLLII
                     LASFLPVFFLDDKEARLFDPLAYSKTFAMAFSTLLTLCVLPIAMIWVFKGHVVRGHSR
                     FELTFTNWYRRATQWFVRYRYAFLAVNVVLLVAAVVLLQGFRRDFMPRMEEGSILYMP
                     TTLPGLSVADAQNLLVKQDQILKSFPEVERVFGKAG"
BASE COUNT          588 a         1114 c         1083 g          565 t
ORIGIN      
        1 ccgtgatcga catgcagtcg ctgaaagcga tcaagacgat cgagggactg cacgagccgc
       61 tcaacatcac gttcacgccc gacggacaga aggcgtacat cggcaactac ggcgcacact
      121 gggtcggcgt cgtcgacgtc acgcgccacg agctcatcaa gaagatcaac gtcgacacgg
      181 tgccgggcgt cgcgcgcctc gatccggcga agtacctcac cgagatcaag ggcatcttca
      241 acgccacgcc gactccggac ggccggttcc tgtacgcggc cgacggcgat ctcggcgtgg
      301 tcggcgtcat cgacacgcgc gaggacaaag tcgtcaaggt catccgcgtc ggcccagatc
      361 cgtggcgcct gtacatgtcg catgacggca agtacggcat caccgcgaac aacggcgaca
      421 gcacgatctc gatcatcgac atcgcgaaga acgccgtcgt cgccacgtac ccggtaggcc
      481 cggatctcac gggcatcaac ttcgccgcag gcaaagcgtt cgtgatcagc tcgacgacgg
      541 gtttcctgta cgtgttcgac atggcgacgc tgaagcccgc gggccgcatc aagatcggca
      601 acaacctgtt tttgcagacg gcgacgaccg acacggccga tcagaagctc tatctcggca
      661 gcgcgacgga caactcggtc tacatcatcg acgccgcaac cgaggccgtg gagcgagtgg
      721 ccaacgtcgg tcagttcccg tggggcactc acatcatgga cagcaaggac aactactgcc
      781 attgatgatc gctacggccg cgccgttgcg attggtgggc cggctgtgcg gcgttgtgtt
      841 gctcggcatc gcggccggga tcttcatctt tcggagcgcg ctggcccagg agccgcagcc
      901 ggaaccgccc gactcgccgc ctgattcggc ggctgctccc gcggaaggcg gtgtccgggc
      961 gcgcacgcgt ctggtccggc ggtcggcgac ggccgtcacc gacgagcaag cctcggaatt
     1021 gacgctgacg cttacggtcg tttcgttccg gccgattcag acttgggtcc gcacggcggg
     1081 cgtgctcgac gcggccggca aggtgctgac ggcacagctg cgctcgcccg aggccgagct
     1141 ggtcgaggtc ggccagcgcg tccgcacttt ctcggtcaac acgcgaacgc aaatggtgca
     1201 ggcgaagatc acgcgagtga cgccgcggcc cggcggcgcg acggtcgaag ccacgctcgc
     1261 ggccgcggtc gcgaacgacg gcgcgcggca tctcatggag atcgtgaccg agaagggacc
     1321 gctcctgtcg gtgccgaacg agtcgatcat cgaagaggcc tacgcccaag tcgtctacct
     1381 caagctcccg agcggcgaat tcgcgccgcg cacgattgag accggcatca cgggtgagct
     1441 ctacacggag atcgtcgacg gcctcgagga cggcgacgag gtcgtctcga tcggcagctt
     1501 cttcgtcgac gccgacaaca agctcaaggg cgggccggcc gagccatgaa tcccgcgttg
     1561 ttcgagggca tcgttcgcgc gcgctggctc atcgcgctat tgatcctggc ggggatcgtg
     1621 gcgagcgtgt tcgcgatgcg cgcggcgccg ctcgacgcga tacccgatac gtcagacccg
     1681 caggtgatcg tctacgcgaa gtgggcgcgc agccccgagc tgctcgagga gcaagtcacg
     1741 gagccgatcg tgaaggcgct gatcggctcg cccgacgtcc gctcgatccg cgcgacgtcg
     1801 cacatgggct actcgttcat ctacgtgatc ctgaagagca ctgccgaacg cgccgcggtg
     1861 cggcagctga tcgcggaccg cctcgcgacg atccgccagc tcctgcccgc cgacgcgacg
     1921 gtgtcgatcg ggccgaacgc gagcggcatc ggctggatct accagtacgc gctgatcgat
     1981 cacgaaaagc tgcgcgaccc gcgcgagatg cggctgctca acgagacgca gctgaagccc
     2041 gcgctgcagg cggttcccgg cgtcgccgag gtcgcctccg tcggcggatt ggagaagcag
     2101 taccagctca agctctttcc gccgctgctc gcggacgcgg ggatctcgct gcgcgagctc
     2161 accgtggcgc tgcgcgacgc gctcgaggag gccggcggcc gcgtcctcga ggtgacgaac
     2221 cgcgactatc aaatacgcgg cgtcgtgaac cacgacagca tcgacgacct cgaggcgttg
     2281 atcgtcgggc acgccccgga cggccgcgcg atcagggtca aggacatcgg ctacatccag
     2341 atcggttacg accagcggcg cggcatcgcc gatctgaacg gcgaaggcga agtcgtcggc
     2401 ggcatcgtga tcatggagca gtcgcagaat gcgctcgaga tcacggccgc gctcaagaag
     2461 cggctcgccg aggtcgcggc gacgctgccg cagggcatcg agatcgcgac cacctatgat
     2521 cgctcgcagc tgatctgggg cacgctgcgg catttcttca tcacgctcgc ttacgagctt
     2581 ggcgtcgtga tcctcgtcac ggcactcttc ctgcgcaacg ctcgcacctc gctcgcgccc
     2641 gtctcgatcc tgctgctcgg cgtgctgttc acggcgattc cgatggcgct cttgaatcag
     2701 accatcaacc tgttctcgct cgccggactc ttcatcgcga tcggcgagat ggtagacgcg
     2761 acgatcgtca tcgtcgaaaa cagcaccgcg gagctcgccg cgcaccccaa cgcgagcgag
     2821 accgagaagc ggcagatcgt cctgcgctcg atcgcgggcg tcgcgaaacc gttgctgttc
     2881 tcgttgctga tcatcctcgc ctcgttcctg ccggtgttct tcctcgacga caaagaagcg
     2941 cggttgttcg atccgctcgc gtacagcaag acgttcgcga tggcgttctc gacgctgctg
     3001 acgctgtgcg tcctgccgat cgcgatgatc tgggtcttca aaggccacgt ggttcgcggc
     3061 cattcgcggt tcgagctcac gttcacgaat tggtaccggc gcgcgaccca gtggttcgtg
     3121 cgttatcgct acgcgttcct cgcggtcaac gtcgtcctgc tcgttgcggc cgtcgtgctg
     3181 ctgcaggggt tccgcaggga cttcatgccc cgcatggagg agggctcgat tctctacatg
     3241 ccgacgacct tgccgggcct ctcggtcgcg gacgcccaga acctgctcgt caagcaggac
     3301 cagatcctca aatcttttcc cgaggtcgag cgcgtcttcg gaaaggccgg
//
LOCUS       DASSNN010000155         5824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_897807, whole genome shotgun sequence.
ACCESSION   DASSNN010000155 DASSNN010000000
VERSION     DASSNN010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897807"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFL84_02700"
     CDS             <1..247
                     /locus_tag="VFL84_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7607561.1"
                     /translation="VPEIGWVLASWSHGRGYATEAARAAIDWLEGALGPRRTVCIIDD
                     ANGASVRVAEKLGFREFARSIYKGSPIVMFERNAGAS"
     gene            280..1503
                     /locus_tag="VFL84_02705"
     CDS             280..1503
                     /locus_tag="VFL84_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607562.1"
                     /translation="MVSVRVRQALQVAAALLALAAYANALRAADDAIADRERQFLATI
                     QEILARDGPYSPELLAPLAALIELYRERENDALAAVTIERARQVVRVNDGLHALEQVP
                     YLEQLIRIERARGNHTTAWNLQQELLALVRRHPEDLRTVPVLREDAERHMEVLAQYLD
                     GVRPPELYLGCYYQGDCDAGQRRIVAQGMIADAGRSYMEAIAVLRRNELYGSDELREL
                     EVARLRGLDLVRTQFGGELGAYVVYYPVRQSLYRLYEYETAASRPLLSQANAAVQIAD
                     WELLYSNNGDAIDRYALVWRTLQKTGASQAQIDELFLPDLPVVLPAFQPNPLARDEGQ
                     SATGHIDVAFEITKYGRGRDVEILDAANATREAKNRLVSFISLSRFRPRPANGEFVDN
                     SRVVLRYYLYAGASD"
     gene            1609..3036
                     /locus_tag="VFL84_02710"
     CDS             1609..3036
                     /locus_tag="VFL84_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607563.1"
                     /translation="MVTLGMRRLESAAAIAAALVLAVVTSPLVAQSLSADDRELEQIQ
                     EILSRDGPYAPELLGPLSRLGLIYQEGEDYALALVTLERAQHIVRVNNGLHSMDQVPV
                     MRQLIAIEDARGNHEGAWDRQQDLLQLLRRHPDDVRTVPVLRDIADKQMEVLASVIAG
                     ERPPEVVLGCFYKQWPTQAEGSCEAGSKSTVVQGMLAEAQRTYLEAIAVLLRQGLYDS
                     DDLRAIELQVLRGVDLMRSRYYKGPTARPVPMAPAYVGASNLEPWRSRMAAVAELAEW
                     ELPYPEEPSLQGDDDDHVLTKQVHIMDPYHRGRQSLRRLYAYGAASSDSSVEQAEAVA
                     QMADWDLLYSHNGRAIESYDVARGMLDEAGVPASSVEQLFAPPTPVVLPAFQPNPLAR
                     DESRVATGYIDVAFEITKYGRSRAVEILDSANASHAAKQDLIALIRVNRYRPQLTDGV
                     FADATPVRLRYYLYEERDAPSLATN"
     gene            3150..4529
                     /locus_tag="VFL84_02715"
     CDS             3150..4529
                     /locus_tag="VFL84_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015500945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET7607564.1"
                     /translation="MAKKKAKAAMRPAKKTAAKAAPIRKGGKPQLVEPKQVLGDDIDP
                     HHQWKRPLQAPGHTQVDFEERVNFRRLHNYRVARVRQALAGSGLGALLSFDQHNIRYT
                     TSTVIGEWARDKLLRYSLITGNGEPYIWDFGSAAKHHKLYAPWLENDHCRAGMLGMRG
                     AVGGDLTLFRDAAKEIKAILDEEGVGHMPVGVDVVEPPMLFELQKAGIEVRDGQQTML
                     LAREIKSHDEITLLNMASAMVDGVYQDIAEALKPGIKESEIVALAAGRLYEMGSDCVE
                     AINSIAGERCSPHPHNFTDRLIRPGDQAFFDVIHSFIGYRTCYYRTFAVGRATAPQRT
                     AYRKAREWMDNAIALLKPGMGSDEVANALPTAEDIGFGSEIDAFGLNFCHGLGLGLHE
                     RPLISRVTSFREPIELKAGMVFAVETYCPATDGMSAARIEEEVVLTPNGAKIITLYPA
                     EDLPIANPY"
     gene            complement(4544..5035)
                     /locus_tag="VFL84_02720"
     CDS             complement(4544..5035)
                     /locus_tag="VFL84_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7607565.1"
                     /translation="MKRRRMPAARGPAFGVALDDLMGYNLRRAHGVQKQRFSTVFGPL
                     SIRPVTLSALGIIYENSGITQADLGKKLNIKRANMVPVMAELENRGLIARRPSDRDRR
                     VHLVALTPAGMKLTVKLLEMHRRLEEDLARELGLRERDQLLQLLKRFRTLATEPELDD
                     GKD"
     gene            5158..>5824
                     /locus_tag="VFL84_02725"
     CDS             5158..>5824
                     /locus_tag="VFL84_02725"
                     /inference="COORDINATES: protein motif:HMM:NF019255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HET7607566.1"
                     /translation="MRRWPIAAGIAFAALAVAAVAVRGRAPWLTATARGDVLDRYCVG
                     CHNDPERAGDVSFESLDREDLGSHAEVWEAAVRKLRTGLMPPLGEPRPERAVLDAFAA
                     SLEKGLDAASARAPNPGTRPLARLNRTEYANAIRDLLAYDASAIASTLPPDAAVAGFD
                     NNAEGLSVSPTLLEGYATAAMQISRRAVGDRTMGHGETRYAATPGAAQRAPIDGLPLG
                     TRGG"
BASE COUNT         1014 a         1908 c         1976 g          926 t
ORIGIN      
        1 agtgccggag atcggctggg tgctcgcgag ctggtcgcac ggcaggggct acgcgaccga
       61 ggcggcgcgc gcagcgatcg attggctcga gggcgcgctg gggccgcgcc gcacggtgtg
      121 catcatcgac gacgcaaacg gcgcatccgt gcgtgtcgcg gagaagctcg ggtttcgcga
      181 gttcgcgcgc agcatataca agggctcgcc gatcgtgatg ttcgaacgca acgccggagc
      241 cagctgacgt agaatcacac aacgcgttcg agggtggtca tggtctcggt ccgagtccgc
      301 caggcgcttc aggtcgcggc cgcgctgctc gcgctggcag cgtacgcgaa cgcgcttcgc
      361 gcagccgacg acgcgatcgc cgatcgggag cggcaatttc tcgcaacgat tcaagagatt
      421 ctcgcgcgcg acggccccta ctcgccggag ctgctcgcac cgctcgcggc gctgattgag
      481 ctgtatcgag aacgggaaaa cgacgcgctg gccgccgtga cgatcgagcg cgcgcgtcaa
      541 gtcgtgcgcg tcaacgacgg gctacacgcg ctcgagcaag tgccgtacct cgagcagctc
      601 attcgcatcg agagggcgcg cggcaatcac acgacggcgt ggaatcttca gcaagagctg
      661 ctcgcgctcg tcaggcgcca ccccgaggac ttgcgtacgg tgccggtgct gcgcgaggac
      721 gcggagcggc acatggaagt cctcgcacag tatctcgatg gagtgaggcc gcccgagctc
      781 tatttgggct gctactacca aggcgactgc gacgccggac agcgaaggat agtcgcacag
      841 ggcatgattg ccgacgccgg gcgtagctac atggaagcga tcgccgtcct gaggcgcaac
      901 gagctctacg gcagcgacga gctgcgcgag ctcgaggtcg ctcgcttgcg cggcctcgat
      961 ctggttcgca cgcagtttgg cggggagttg ggcgcctacg tggtctacta cccggtgcgc
     1021 caaagcctat atcggctcta tgaatatgaa actgcggcgt cgcgcccgct gctgagccaa
     1081 gcgaatgcgg ccgtgcaaat cgcggactgg gagctgctgt attcgaacaa cggcgacgcg
     1141 atagaccgct acgcgcttgt gtggaggacg ttgcaaaaaa ccggcgcctc gcaagcgcag
     1201 atcgatgagt tgttcttgcc ggacttgccc gtcgtgctgc cggccttcca accgaatcct
     1261 ctggcccgcg acgaaggtca gagcgcgacg ggacacatcg acgtggcgtt cgagatcacg
     1321 aagtacggca gaggccgcga tgtcgaaatt ctcgatgctg caaacgcgac gcgcgaggcg
     1381 aagaatcggc tcgtcagctt catctcgctg agccgcttcc gcccgcgccc ggcgaacggc
     1441 gagtttgtcg acaattcacg cgtcgtcctc cgctattact tgtacgccgg cgcgagcgac
     1501 tgacgagccg cggcccgcgc tttttttctc gctccgagcc gcgaggcggg catagccctt
     1561 gcataggctc cgatggggga ccggatcgca acccatcgga ggtagcgcat ggttacgctc
     1621 ggcatgcgcc gactcgagtc cgccgccgcg atagccgcgg ccctcgtgct cgccgtcgtt
     1681 acgtcccccc tcgtcgcgca gtcgctgtcg gcagacgacc gggagctcga acaaattcag
     1741 gaaatccttt cacgcgacgg cccgtacgcg cccgagctgc tcggaccctt gtcgcgcctc
     1801 gggctcatct atcaggaagg cgaggactat gcgcttgcgc tcgtgaccct cgagcgcgcg
     1861 cagcacatcg tgcgcgtcaa caacggattg cattcgatgg accaggtgcc ggtcatgcgt
     1921 cagctcatcg ccatcgaaga cgcgcgcggc aaccacgaag gagcgtggga ccgccagcaa
     1981 gacctgttgc agttgctgcg gcgccacccc gacgacgttc ggaccgtgcc cgtcctgcgc
     2041 gacatcgccg acaagcagat ggaagtgctc gcctcggtca tcgccggcga gcggccgccc
     2101 gaggtcgttc tcggctgttt ctacaagcag tggccgacgc aagcggaggg tagctgcgaa
     2161 gcgggcagca agagcaccgt cgtccaggga atgttggccg aggcgcaacg aacgtatctg
     2221 gaggcgatcg cggttctgct gcgccaaggc ctctacgaca gcgacgatct gcgtgcgatc
     2281 gagctgcaag tgctgcgcgg cgtcgatctg atgcgttcgc gctactacaa aggtccgacc
     2341 gcgcgtcccg tgccgatggc gcccgcgtac gtcggcgcgt cgaacttgga gccgtggcgc
     2401 agccgaatgg cggcggtcgc cgagctcgcg gaatgggagc tgccgtatcc ggaagagccg
     2461 tcgctgcaag gtgacgacga cgatcacgtc ttgacgaagc aggtgcacat catggatccg
     2521 tatcatcgcg gccggcagag ccttcgccgc ctctatgcgt acggcgctgc gagctccgac
     2581 tcgtcggtag agcaggcgga agccgtcgcg cagatggccg actgggacct gctctattcg
     2641 cacaacggcc gcgcgatcga gagctacgac gtcgcgcgcg ggatgctcga cgaagccggc
     2701 gtcccggctt cgtcggtgga gcagctgttc gcgccgccga cgcccgtcgt gctgcccgca
     2761 ttccagccga atccgctcgc gcgcgacgag agtcgcgtcg cgacggggta catcgacgtc
     2821 gcgttcgaga tcacgaagta cggccggagc cgtgccgtcg agatcctcga ctccgcgaac
     2881 gcaagccacg ccgcgaagca agacctgatc gcgctgatca gggtcaaccg ataccggccg
     2941 cagctgacgg atggcgtgtt cgccgacgcc acgcccgtcc ggttgcgtta ctacctctac
     3001 gaagagcgcg acgcgccgag cctcgctacg aactagcgct ccgccaccgc cgagccgtcc
     3061 gccgggctgt cgcgagccgc ccgcgcgcgc gtacaatctc cgggccaacc gcgcgtggtt
     3121 cggcagcgct cattcaatag aggacgacaa tggctaaaaa gaaggcgaag gcggcgatga
     3181 ggccggccaa gaagacggcg gcgaaggcgg caccgattag aaagggcggc aagccgcagc
     3241 tcgtcgagcc gaagcaggtg ctcggcgacg acatcgaccc gcatcatcag tggaagcgcc
     3301 cgctgcaggc gccgggtcat acgcaagtcg acttcgagga gcgcgtcaat ttccgccgcc
     3361 tgcacaacta ccgtgtcgcg cgcgtgcgcc aggcgctcgc gggcagcggc ctcggcgcat
     3421 tgctgtcgtt cgatcagcac aacatccgct acacgacgag caccgtcatc ggagaatggg
     3481 cgcgcgacaa gctgctgcgc tactcgctga tcaccggcaa cggcgagccg tacatctggg
     3541 atttcggctc ggccgcgaag catcacaagc tctacgcgcc gtggctcgag aacgatcact
     3601 gccgagcggg catgctcggt atgcgcggag cggttggagg agacctcacg ctatttcgcg
     3661 atgcggcgaa agagatcaag gcgatcttgg acgaggaagg cgtcggccac atgcccgtcg
     3721 gcgtcgacgt cgtcgagccg ccgatgttgt tcgagctgca gaaggccggc atcgaagttc
     3781 gcgacggcca gcagacgatg ttgctcgcgc gcgagatcaa gtcgcacgac gagatcacgc
     3841 tcttgaacat ggcgtcggcg atggtcgacg gcgtgtacca ggacatcgcc gaagcgctga
     3901 agcccggcat caaggagtcg gagatcgtcg cgctcgccgc gggccggctc tacgaaatgg
     3961 gctcggattg cgtcgaggcc atcaactcga tcgcgggcga gcgctgctcg ccgcatccgc
     4021 acaacttcac cgatcgattg attcgccccg gcgaccaggc gttcttcgac gtcatccatt
     4081 cgttcatcgg ctatcgcacg tgctactacc gcacgttcgc ggtcggccgc gcgacggcgc
     4141 cgcaacgcac ggcgtatcgc aaggcgcgcg agtggatgga caacgcgatc gcgctcttga
     4201 agccgggcat gggcagcgac gaggtcgcga acgcgctgcc gacggccgag gacatcggct
     4261 tcggcagcga gatcgacgcg ttcggcttga acttctgcca cggtctcggg ttgggcttac
     4321 atgagcggcc gctgatctcg cgcgtcacgt cgttccgcga gccgatcgag ctcaaggccg
     4381 gcatggtgtt cgcggtcgag acttattgtc cggcgacgga cggcatgtcg gccgcgcgca
     4441 tcgaggaaga agtcgtgctg acgccgaacg gcgcgaagat catcacgctg tacccggccg
     4501 aagatctgcc gatcgcaaat ccgtactgaa gcgtagagct agctcagtcc ttgccgtcgt
     4561 cgagctcggg ctccgtcgcg agcgtgcgaa agcgcttcaa cagttgaagc agctgatcgc
     4621 gctcgcgcag tccgagctcg cgcgccaagt cttcctcgag ccggcgatgc atctcgagca
     4681 gcttgacggt cagcttcatt cccgcaggcg tcagcgcgac gaggtgaact cgccggtccc
     4741 tgtcgctggg gcgccgcgcg atgaggccgc ggttctcgag ctcggccatg acgggaacca
     4801 tgttcgcgcg cttgatgttg agcttcttgc cgaggtcggc ctgcgttata cctgagttct
     4861 cgtagatgat gccgagcgcc gacagcgtga cgggccggat gctcaggggc ccgaacacgg
     4921 tcgagaagcg ctgcttctgc acgccgtggg cgcgccgcaa gttgtagccc atgagatcgt
     4981 cgagcgcgac cccgaaggcc gggccgcgcg ccgccggcat tcgccgccgt ttcacatcag
     5041 tcgccagagc aggccctcgc gattcatccg gattcgccga agggcgcgat attggtagat
     5101 gccccggcgg cttgcaagcg cggtatgctt cgaagcggga ctcctcgggt cgtcacgatg
     5161 cgacgctggc ccatcgccgc cggaattgcc ttcgccgcgc ttgctgtcgc ggccgtcgcc
     5221 gtgcgcggcc gggcgccgtg gctgacggcc actgcgcgcg gcgacgtgct cgatcgctac
     5281 tgcgtcggct gccacaacga ccccgagcgc gcgggcgacg tgtcgttcga gagcttggat
     5341 cgcgaagacc tcggcagcca cgccgaggtc tgggaagcgg ccgtccgcaa gctgcgcacg
     5401 ggcttgatgc cgccgctcgg cgagccgcgg cccgagcgcg cggttctcga cgcgttcgcc
     5461 gcctcgctcg agaaagggct cgatgcggcc tccgcgcggg cgccgaaccc gggaacgcgg
     5521 ccgctcgccc ggctgaatcg caccgagtac gcgaacgcga tacgcgatct cctggcctat
     5581 gacgcgagcg cgatcgcgag cacgttgccg cccgatgcgg cggttgctgg gttcgacaac
     5641 aacgccgagg gactcagcgt ctcgccgacg ctgctcgaag gctacgcgac ggcggcgatg
     5701 cagatcagcc gccgcgccgt cggcgatcgc acgatgggtc acggcgagac gcgctacgcg
     5761 gcaacaccgg gggcggcgca gcgcgcgccg atcgacggcc tgccgctcgg cacgcgcggc
     5821 gggc
//
LOCUS       DASSNN010000156         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1276414, whole genome shotgun sequence.
ACCESSION   DASSNN010000156 DASSNN010000000
VERSION     DASSNN010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276414"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..667)
                     /locus_tag="VFL84_02730"
     CDS             complement(101..667)
                     /locus_tag="VFL84_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7607567.1"
                     /translation="MSGTPFRRIVTGHDARGRSVIIEDGPPPRVARIGGELGPLFYEV
                     WNTRETPASIDRASGEPHEDGITLAPPKNGTRIRVLDIPPEDPRLSTLSPEQARAHFA
                     EVGAADAASHTGSGSRHAFMHRTETVDYGIVLEGEITLIMDEGETVARAGDIVIQRGT
                     NHGWANRSGKPCRIAFILIDGRFDEELA"
     gene            complement(672..1850)
                     /locus_tag="VFL84_02735"
     CDS             complement(672..1850)
                     /locus_tag="VFL84_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET7607568.1"
                     /translation="MALFQYFPNNYVWNLSVNMALASGGQIGEIEDMCRPLLEAAARG
                     EDAGTPEFLAQWVAVADNLVGLAAEDEAKGRLLSAAAKLQRAALYYLVAERMQGHGHP
                     GREATYAKAQRTFRHAIEIGRENAERVEIPYAGTVIPALFSRPEDSGGRAPCVLYVNG
                     LDSCKELLYWSWLPHALARRGVATLSIDQPGTGESLRLHGLHATHESERWASVCYEWL
                     AARNDVDAARIGIVGISLGGYFAPRAMAFEPRFASGAAWGANHDWAEVQRKRLQREGE
                     HPVPHYWNHVMWVFGASGIDDFLAKSAGMHLDGILDRIEAPFLVVHGESDRQIPREYA
                     RRTYAELVNSPRRELKILTAREGGVEHVGADNMTFARDYIADWFAETLGGSVGGQPGA
                     "
     gene            complement(1855..>2568)
                     /locus_tag="VFL84_02740"
     CDS             complement(1855..>2568)
                     /locus_tag="VFL84_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7607569.1"
                     /translation="DVIALAAASAGDVDALFAKVEAAGCPIVHPPRPLETLGGGYGFR
                     FFSPDGLPFEISSGVERGKARRLAPREAIPERISHIVLHSPDHKAAAQFFVDVLGFRV
                     SDWLGNFMVFLRCNEWHHRIALLPGPPCLNHVAYDVANIDEMMRGIARLRGEGVEIRW
                     GPGRHTAGNNAFSYFVTPNGFAVEYTAEVERVDPATWQPTVHQPGPGIMDQWGLGVGG
                     PETMPPPEPDKGLFQPAGV"
BASE COUNT          382 a          883 c          877 g          426 t
ORIGIN      
        1 gagctcgaga tcccgcgaca cgacgagcag ccggccgtcg gcggcgccgg tgagctcggt
       61 ggccagcttc atgtgcgtcc cgtccggcga ttcgcttcgc tcatgcgagc tcctcgtcga
      121 atcggccgtc gatcagaatg aacgcgatgc ggcacggttt gccggatcgg ttggcccagc
      181 cgtgattcgt gccgcgttgg atcacgatgt cgccggcacg ggctacggtc tcgccctcgt
      241 ccatgatcag cgtgatttcc ccttcgagca cgatgccgta gtccaccgtc tcggtgcggt
      301 gcatgaacgc gtgccgggac ccgctccccg tgtgcgaggc cgcgtcggcg gcccccacct
      361 cggcgaagtg cgcccgcgcc tgctcgggcg agagcgtgct caagcgcgga tcctcgggcg
      421 ggatgtcgag cacccgaatg cgcgtgccgt tcttcggcgg cgcgagcgtg atgccgtcct
      481 cgtgcggctc gccgctcgca cggtcgatac tcgccggcgt ctcgcgtgtg ttccagacct
      541 cgtagaacag aggcccgagc tcgccgccga tccgtgccac acgcggcggc ggcccgtcct
      601 cgatgatgac cgagcgtccg cgagcatcgt ggcccgtgac gatacggcga aagggtgttc
      661 cactcattcg tctatgctcc cggttggcct ccgacggaac cgccgagcgt ctcggcgaac
      721 cagtcggcga tgtagtcgcg cgcgaaggtc atgttgtcgg cgccgacgtg ctcgacgccg
      781 ccttcgcgtg cggtgaggat cttgagctcg cggcgcgggc tgttcacgag ctctgcatac
      841 gtgcgccgcg cgtactcgcg aggaatctgc cggtcgctct cgccatgcac gacgagaaac
      901 ggagcctcga tccggtcgag tatcccgtcg agatgcatgc ccgccgattt cgctagaaag
      961 tcgtcgatgc cgctcgcgcc gaacacccac atcacgtggt tccagtagtg cggcacggga
     1021 tgctcgcctt cgcgctgcag gcgcttgcgc tgcacctcgg cccagtcgtg gttcgcgccc
     1081 caggccgcac cgcttgcgaa gcgcggctcg aacgccatcg cacggggcgc gaagtagccg
     1141 ccgagcgaaa tgccgacgat gccgatgcgc gcggcgtcga cgtcgttgcg cgccgcgagc
     1201 cattcgtagc acacgctcgc ccagcgctcg ctctcgtgcg tggcgtgcaa gccatgcagc
     1261 ctcagcgatt cgccggtgcc gggctggtcg atcgaaagcg ttgcgacgcc gcggcgcgcg
     1321 agcgcgtgcg gcagccagga ccagtagagc agctccttgc agctgtcgag gccgttgacg
     1381 tagagcacgc atggcgctcg gcctccgctg tcttcggggc ggctgaacaa ggccggaatg
     1441 acggtgccgg cgtacggaat ctcgacccgc tccgcgttct cgcggccgat ctcgatcgca
     1501 tgacggaatg tgcgctgcgc tttcgcgtac gtcgcctcgc ggcccggatg cccatgaccc
     1561 tgcatccgct ccgcaacgag atagtagagc gcggcgcgct gcagctttgc cgcggccgag
     1621 agcagcctcc ctttcgcttc gtcctctgcg gcgagcccga cgaggttgtc ggcgacggcg
     1681 acccattgcg cgaggaactc cggcgtgccc gcgtcctccc cgcgcgccgc ggcttcgagc
     1741 aacgggcggc acatgtcctc gatctcgccg atctgcccgc cgctcgcgag cgccatgttg
     1801 acggaaagat tccagacgta gttgttcgga aagtactgaa aaagcgccat cgagctagac
     1861 gcccgcgggc tggaagagcc ccttgtcggg ctccgggggc ggcatcgttt ccgggccgcc
     1921 gacgccgagg ccccactgat ccatgatgcc ggggccgggc tgatggacgg tcggctgcca
     1981 cgtcgctggg tccacgcgct cgacctcggc ggtgtattcg acggcgaagc cgttcggcgt
     2041 gacgaagtag ctgaacgcgt tgttgccggc cgtatgccgg ccgggtcccc accgtatctc
     2101 gacgccttcg ccgcggagcc gcgcgatgcc gcgcatcatc tcgtcgatgt tcgcgacatc
     2161 gtaggcgacg tgattgaggc acggcggacc gggcagcaat gcgatgcggt ggtgccactc
     2221 gttgcagcgc agaaacacca tgaagttgcc gagccagtcg ctcacgcgga agccgagcac
     2281 gtcgacgaag aactgcgcgg ccgccttgtg gtcgggcgag tgcagcacga tatggctgat
     2341 tcgctcgggg atcgcttcac gcggagcgag ccgccgcgcc ttgccccgct cgacaccgct
     2401 cgagatctcg aacggcaagc cgtcgggcga aaagaaccgg aagccgtagc cgccgccgag
     2461 cgtctcgagc gggcggggcg gatggacgat ggggcaaccc gcggcctcga ccttcgcgaa
     2521 gagcgcatcg acgtccccgg cgctcgccgc ggcgagcgcg atcacgtc
//
LOCUS       DASSNN010000157         5088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_771717, whole genome shotgun sequence.
ACCESSION   DASSNN010000157 DASSNN010000000
VERSION     DASSNN010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771717"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="VFL84_02745"
     CDS             <1..1525
                     /locus_tag="VFL84_02745"
                     /inference="COORDINATES: protein motif:HMM:NF017142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HET7607570.1"
                     /translation="FVSPTQTNQGRIVATREMLNLQWEAVLLYPAGYYVSRIRVAPSV
                     KLPEGWRFAAALDGAVTTGATTRFAETDLETLVDSPLFAGEHYRLVDLDRGAARPVRL
                     NMFADRPDQLAATTEQLDQHRNLVQQAYRLFGSQHFDHYDFLLALTDRMGGIGLEHHR
                     SSENGVDSGYFTDWSTARSERDTLPHEFVHSWNGKFRRPADLWTPSYDTPMRGSLLWV
                     YEGLTQYYGFVLAARSGLWSLEDARAALAMTAATYQHRVGREWRSVADTTNDPILSAR
                     RPAPWRSWLRNEDYYSEGLLVWLDADTLIRERTNGRSSLDDFARAFFGGDDGSWRPVT
                     YTLDDVVAALNRVHENDWARFFRERIYEAGAEPPLDGLTRGGYRLVFKEERSEFQREA
                     EVYGRNADFSYSLGLSFGENGNVTGVQWDGPAFNQGITVGTQVVAVNGVSYGNESLRR
                     AVTAAKTARAPIELLLRDGDQYRTIALDYRGGLRYPHLERDEAASDRLTEILSPRAH"
     gene            1573..2571
                     /gene="dusB"
                     /locus_tag="VFL84_02750"
     CDS             1573..2571
                     /gene="dusB"
                     /locus_tag="VFL84_02750"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET7607571.1"
                     /translation="MLIGPLKIDPPLILAPMAGVTDKPFRLLCKRLGAGYAVSEMTSS
                     DPRLWRTRKSGLRLDHAGEPAPIGVQIAGAESQALAAAARHAVEHGAEIVDINMGCPA
                     KKVCNAWAGSALLKDEALVERLVRAVVAAVDVPVTLKIRTGWDRANRNGVRVARIAED
                     AGVSALAVHGRTRDMLYAGDAEYETIAAIKQAVRIPVIANGDVTSPHKAREVLAATGA
                     DALMIGRAAQGRPWIFREIAHFLATGQAAPPPAVADVRDTLLEHVRALHEFYGEPAGV
                     RIARKHLAWYAKDRPENAAFRAVVNRAETAEEQLRLSRDYFDGLELAATAPFVAAA"
     gene            complement(2517..2858)
                     /locus_tag="VFL84_02755"
     CDS             complement(2517..2858)
                     /locus_tag="VFL84_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607572.1"
                     /translation="MKATEVRILPALDPGESYVDPAFMLVETALDDNGDPVTTIEHVA
                     SRLVGDKTRWQVATLGQSVPLSHAAALEWAVSFAASRNIPVVYERDGTLPPAHAAATN
                     GAVAASSSPSK"
     gene            complement(2992..3693)
                     /gene="uppS"
                     /locus_tag="VFL84_02760"
     CDS             complement(2992..3693)
                     /gene="uppS"
                     /locus_tag="VFL84_02760"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET7607573.1"
                     /translation="MHTAIIMDGNGRWAERRGLVRSAGHRAGAKAVRRIVEAATRGPI
                     DILTLYAFSSDNWSRPPQEVASLMRLLKRYLVSETARCLDNGVRLSVIGRRDRLSRDL
                     VRTIENTERLTIDGTRLHLRLAIDYSARAAIAEAAARTRVPEDFERALAAAVHSDVDA
                     PVDLLIRTGGERRLSDFLLWECAYAELVFVDTFWPDFDEAAFDAALREYGRRDRRFGR
                     VSSSPEPQPGYSRAE"
     gene            complement(3699..4091)
                     /locus_tag="VFL84_02765"
     CDS             complement(3699..4091)
                     /locus_tag="VFL84_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET7607574.1"
                     /translation="MAAKTVLKRLEAAASAPDNQRLRAVAGYFADREAGETDRLIYER
                     VRLGIMSALSVNRSMTFAELKELLKTTDGNLSIHARKLEDAGYVACKKRFSARVPKTE
                     FSLTAKGKRVFARHLEHMEALIQAAKGT"
     gene            complement(4081..4692)
                     /locus_tag="VFL84_02770"
     CDS             complement(4081..4692)
                     /locus_tag="VFL84_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607575.1"
                     /translation="MSASPKRPRPIAPTPIDSGALENLRYIRSTIEAADTFTTVPGYG
                     CIAMGVTALAAVGVESIPRLTPYWLGIWVAAAMVACATALWFMEQKARAQGLSLRRAV
                     ARRFFMTLAPAFIAGAILTAALAGRVDRELITGMWLLLYGTGLAACGLFAIRAVSTAG
                     LAFMALGTATLWLPPGSAHIVLALGFGGIHLALGVNIVRHHGG"
     gene            complement(4895..>5088)
                     /locus_tag="VFL84_02775"
     CDS             complement(4895..>5088)
                     /locus_tag="VFL84_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HET7607576.1"
                     /translation="DLKIKLMGAIVAISAVELLKAFINVDENTPWQTLAWKVGIHVTF
                     VVSGVLFAVTDRITDRGGH"
BASE COUNT          836 a         1668 c         1762 g          822 t
ORIGIN      
        1 gttcgtgtcg ccgacgcaga cgaaccaagg ccgcatcgtc gcgacgcgcg agatgctgaa
       61 cctgcagtgg gaggcggtat tgctctatcc cgcgggctac tacgtgtcgc gcatccgcgt
      121 tgcgccgagc gtcaagctgc ccgagggctg gcggttcgcg gccgcgctcg acggcgccgt
      181 cacgaccggc gcgacgacgc gctttgcgga gacggacttg gagacgctcg tcgattcgcc
      241 gttgttcgcg ggcgagcact atcggctcgt cgacctggat cgcggcgcag cacggcccgt
      301 gcgcttgaac atgttcgcgg accggcccga tcagctcgcg gcgacgacgg agcagctcga
      361 tcagcaccgc aatctcgtgc agcaggcgta tcggctgttc ggctcgcagc acttcgatca
      421 ctacgatttc ctgctcgcgt tgacggaccg catgggcggc atcggcctcg agcaccaccg
      481 ctcgagcgag aacggcgtcg acagcggcta cttcacggat tggagcaccg ccaggagcga
      541 gcgcgacacg ctgccgcacg aattcgtcca ttcctggaac ggcaaattca ggcggcccgc
      601 ggatctctgg acgccgagct acgacacgcc gatgcgcggc agcctgctct gggtctacga
      661 agggctcacg cagtactacg ggttcgtgct cgcggcgcgc tcgggactct ggtcgctcga
      721 ggacgcgcgc gccgcgctcg cgatgacggc cgcgacgtac cagcaccgcg tcggccgcga
      781 gtggcgctcc gtcgccgaca cgacgaacga tccgatcctg tccgcgcgcc ggccggctcc
      841 gtggcgaagc tggctgcgca acgaggacta ctactcggaa gggttgctcg tctggctcga
      901 cgccgacacg ctgattcgcg agcgcacgaa cggccgcagc tcgctcgacg acttcgcgcg
      961 cgcattcttc ggcggcgacg acggctcgtg gagacctgtc acgtacacgc tcgacgacgt
     1021 agtagccgcg ttgaatcgcg tgcacgagaa cgactgggct cggttctttc gcgagcgcat
     1081 ctacgaggcc ggcgccgagc cgccgctcga cgggttgacg cgcggcggct atcggctcgt
     1141 gttcaaggaa gagcgcagcg agttccagcg cgaagcggaa gtctacggcc gcaacgccga
     1201 cttcagctat tcgctgggtc tctccttcgg cgagaacggc aacgtgacgg gtgttcagtg
     1261 ggacggtccc gcgttcaacc agggcatcac ggtcggcacg caagtcgtcg cggtcaacgg
     1321 cgtctcgtac ggcaacgagt cgctgcgccg cgccgtcacg gccgcgaaaa cggcgcgagc
     1381 gccgatcgag ctgctgctcc gcgacggcga tcagtatcgg acgattgcgc tcgactaccg
     1441 cggcggcttg cgttatccgc acctcgagcg cgacgaggcg gcgagcgatc gcctgaccga
     1501 gattctgtcg ccgcgcgcac attgacggcc gacccgaagt gctagactcg cgctcccctg
     1561 gaggtcacac gcgtgctcat cggccccttg aagatcgacc cgcccctgat cctcgcgccg
     1621 atggcaggcg tgacggacaa gccgtttcgc ttgctatgca agcggctcgg cgcgggctat
     1681 gccgtctccg agatgacgag ctccgatccg aggctgtggc gcacacgcaa gagcgggctt
     1741 aggctcgatc acgccggcga gccggcgccg atcggtgtgc agattgcggg cgcagagagc
     1801 caagcgctcg ccgccgcggc gcggcacgca gtcgagcacg gcgccgagat cgtcgacatc
     1861 aacatggggt gccccgcaaa gaaggtctgc aacgcgtggg cgggctcggc gctgctcaag
     1921 gacgaggcgc tcgtcgagcg cctcgtgcgc gccgtcgtcg ccgcggtcga tgtgcctgtc
     1981 acgctgaaaa ttcgcacggg ttgggaccgc gcgaatcgca acggcgttcg agtcgcgcgc
     2041 atcgccgagg acgccggcgt gagcgcgctc gccgtgcacg gcaggaccag agacatgctg
     2101 tatgccggcg acgccgagta cgagacgatc gcggcgatca agcaggcggt gcggattccg
     2161 gtcattgcga acggcgacgt cacgtcgccg cacaaagcgc gcgaggtgct cgcagccacg
     2221 ggcgccgatg ctctcatgat cggccgcgcg gcgcagggca gaccgtggat attccgcgag
     2281 atcgcgcact tcctcgcgac gggccaagcc gcgccgccgc cggccgttgc cgacgtgcgc
     2341 gacacgttgc tcgagcacgt gcgcgcgctt cacgagttct acggcgagcc ggccggagtg
     2401 cgcatcgcac gcaagcacct cgcgtggtac gccaaggaca ggcccgagaa cgccgcgttc
     2461 cgcgcagtcg tcaaccgtgc cgagacggcc gaagagcagc tgcgtctatc gcgcgactat
     2521 ttcgacgggc tcgagctcgc cgccacggcg ccattcgtcg cagcggcgtg agccggcggc
     2581 agcgtgccgt cacgctcgta gacgacggga atgttgcggc tcgccgcgaa cgacacggcc
     2641 cattcgagcg cggccgcgtg cgagagcggc acggactgac cgagcgtcgc gacctgccag
     2701 cgcgtcttgt cgccgacgag gcggctcgcg acgtgctcga tcgtcgtgac gggatcgccg
     2761 ttgtcgtcga gcgccgtctc gacgagcatg aacgccgggt ctacgtagga ttccccggga
     2821 tcgagcgcgg ggagaatgcg aacttccgtg gccttcattg ttgttttcct tggccgtgcg
     2881 gaaagtatcg ctcatccggc gttgcgcggc gccccggttt ggacataaga gggagagcgc
     2941 gctcgaagcg gcgcgcgagc ccgccagcca tggcggttcc ggcggttccg gctattcggc
     3001 tcgcgagtag cccggctgag gctccgggct cgacgagacg cgcccgaagc ggcgatcgcg
     3061 ccgcccatac tcgcgcagcg cggcgtcgaa cgcggcctcg tcgaaatcgg gccagaacgt
     3121 gtcgacgaag acgagctccg cgtacgcgca ttcccagagc aggaaatcgc tgaggcgccg
     3181 ttcgccgccc gtgcggatca gcagatcgac gggcgcgtcg acgtccgaat gaacggccgc
     3241 cgcgagcgcg cgctcgaaat cttccggcac gcgcgtgcgc gctgcggctt ccgcgatcgc
     3301 ggcgcgcgcc gagtagtcga tggcgagccg caagtgcagc cgcgtgccgt cgatcgtgag
     3361 gcgctcggtg ttctcgatcg tgcgcacgag atcccggctc aatcgatcgc gccggccgat
     3421 cacgctcaag cgaacgccgt tgtcgaggca gcgcgccgtc tcgctgacga gatagcgctt
     3481 caagaggcgc atcaagctcg cgacttcttg cggcggccgt gaccagttgt ccgaggagaa
     3541 cgcgtacagc gtcaggatgt cgatcgggcc gcgcgttgcg gcctcgacga ttcggcggac
     3601 ggccttggcg ccggcgcgat ggccggccga acggacgagg ccgcggcgct ccgcccaacg
     3661 accgttgccg tccatgatga tcgcagtgtg catcgcagtt aggtgccttt cgccgcttga
     3721 atcagcgctt ccatgtgttc cagatgacgc gcaaagaccc gcttgccctt ggcggtcaag
     3781 ctgaactcgg ttttcggaac gcgggcggaa aatcgcttct tgcacgcgac gtacccggcg
     3841 tcctcgagct tgcgcgcgtg gatgctcaag ttgccgtccg tcgtcttgag gagctctttg
     3901 agctccgcaa aagtcatgct gcggttgacg ctgagcgcgc tcatgatgcc caagcgcacg
     3961 cgctcgtaaa tcagccggtc cgtctcgccc gcttcgcggt ccgcgaaata cccggccacg
     4021 gcgcgaaggc gctggttgtc gggcgcggag gccgcggctt cgagccgctt caatacggtt
     4081 ttagccgcca tggtgcctca cgatgttgac gccgagcgcc aggtgtatgc cgccgaaccc
     4141 gagcgcgaga acgatgtgag ccgacccggg cggcagccag agcgtggcgg tgccgagcgc
     4201 catgaacgcg aggccggccg tggagacggc gcgtatcgcg aacaggccgc acgccgcgag
     4261 tcccgtgccg tacagcagca gccacattcc cgtgatcagc tcgcggtcga cgcggcccgc
     4321 gagcgcagcg gtcaggatcg cgccggcgat gaacgcgggc gcgagtgtca tgaagaagcg
     4381 cctggcgacg gcgcggcgca agctcaggcc ttgagcgcgc gccttctgct ccatgaacca
     4441 cagcgccgtg gcgcaggcga ccatcgcggc ggcgacccag atgccgagcc aatacggcgt
     4501 caggcgtggt atcgattcga cgccgacggc ggcgagcgcc gtgaccccca tcgcgatgca
     4561 gccgtagccc ggaaccgtcg taaaggtgtc tgccgcttcg atcgtactgc ggatgtaccg
     4621 cagattctcg agggctccgg agtcgatcgg agtcggcgcg attggacggg gacgcttggg
     4681 tgatgcgctc atcgaaggtt ccggcggtct tgcctggatt ctaaggcaag tactttacgc
     4741 cgcaaagtgg gtgggtgtcc gtgaactggt gccgatgttg acatatgtcg aaaacggctt
     4801 gctagcgcgc cgtgcgcgca ggcgaatggt tggctggtgg agcgggggta cgggcgctac
     4861 ggcctgggcc gtctctgcgg gggaccttca tgggtcagtg accgcctcta tcggtgatcc
     4921 gatctgtcac tgcaaatagc acgccagaga cgacgaacgt gacgtgtatg ccgactttcc
     4981 aggcaagtgt ctgccacggc gtgttctcgt cgacgttgat gaacgctttc aagagctcga
     5041 cggccgagat tgcgacgatg gctcccataa gtttgatttt caaatctg
//
LOCUS       DASSNN010000158         2041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_159054, whole genome shotgun sequence.
ACCESSION   DASSNN010000158 DASSNN010000000
VERSION     DASSNN010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159054"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /gene="waaF"
                     /locus_tag="VFL84_02780"
     CDS             complement(<1..832)
                     /gene="waaF"
                     /locus_tag="VFL84_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HET7607577.1"
                     /translation="MATDKVLVVGPSWVGDMVMAQALYRALHAANPHVEIHVVAPPWS
                     LPVLERMPEVARGIELAVGHGELGLGKRLSLARRLRAERYTRAIVLPRSAKAALVPWL
                     ARVPLRTGFRGEWRYGLLNDVRVLEARLDQTVKRFVALGLPREAPLPDRLPSTLQPRL
                     RSDARNLDRLRALHGLTHGARFAALMPGAEYGPAKRWPAAHYGALAAALADSDSDVVV
                     LGSGKERAIGDEVAARAGNDRVRNLCGQTSLADVVDRLAAADVAVSNDSGLLHVAAAA
                     G"
     gene            complement(832..1800)
                     /gene="rfaD"
                     /locus_tag="VFL84_02785"
     CDS             complement(832..1800)
                     /gene="rfaD"
                     /locus_tag="VFL84_02785"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017377933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HET7607578.1"
                     /translation="MIVVTCGAGFIGSNLVRELNARGHDDVVVVDDLSDGRKFVNLVD
                     CRIADYLDKSELLERVKRRDLGKVDAIFHQGACAVTTEWNGRYMMETNYRYSVELLEH
                     CLDKGVPLIYASSAAVYGASTTFSEEDRAAERPLNVYGYSKLLFDQYVRRRIRGRRGR
                     GKKRRAQVVGLRYFNVYGPGEAHKGAMASVAFHLHGQVAATGQAKLFEGSGGYGPGEQ
                     RRDFVHVGDVADVNVWLLEHEKVSGIFNVGTGSSATFNEVAKAIIEWHGRGAIRYIPF
                     PDELRSRYQSFTEADITALRRAGYSRPFRDVRAGIKDYLDALVARP"
     gene            complement(1817..2017)
                     /locus_tag="VFL84_02790"
     CDS             complement(1817..2017)
                     /locus_tag="VFL84_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019557721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HET7607579.1"
                     /translation="MNENLIPANAERVYQVTRADLPLHCPMPSQYLWNSHPKVYLPIE
                     ATGEAKCPYCSSTYRLVEDDTR"
BASE COUNT          339 a          709 c          669 g          324 t
ORIGIN      
        1 cgcccgccgc agcagccacg tgcagcaggc cggagtcgtt gctgaccgcg acgtcggcgg
       61 cggccagccg atccacgacg tccgcgagcg acgtttggcc gcagaggttg cggacacgat
      121 cgttcccggc gcgggccgcg acctcgtcgc cgatggctcg ctccttgccc gagccgagca
      181 cgacgacgtc gctatcgcta tcggcgagcg cggcggccaa cgccccgtag tgcgcggcgg
      241 gccagcgctt ggccggacca tactcggcgc cgggcatcag cgcggcgaag cgcgcgccgt
      301 gggtcaagcc gtgcagcgcg cgcaaccggt cgagattgcg cgcatcgctg cgcagccgcg
      361 gctgcaacgt gctcggcaac cggtcgggca acggcgcttc gcgaggcaag ccgagagcca
      421 cgaaccgctt taccgtctga tcgaggcgcg cttcgagcac gcgaacgtcg ttgagcaagc
      481 cgtaccgcca ctcgccgcgg aatccggtgc gcagcggaac gcgcgcgagc cacggcacga
      541 gcgccgcttt cgcggaacgc ggcagcacga tcgcgcgcgt gtagcgctcc gcgcgaaggc
      601 gccgcgccag agacaagcgc ttgccgagac cgagctcgcc gtggccgacg gcgagctcga
      661 tgccgcgcgc aacctcgggc atccgctcga gcaccggcag cgaccaaggc ggcgcgacga
      721 cgtggatctc gacatgcgga ttcgcggcgt gcagcgctct gtacagcgcc tgcgccatga
      781 ccatgtcgcc cacccacgag gggccgacga cgagcacctt gtcggttgcc atcacggcct
      841 ggcgacgaga gcgtcgaggt aatccttgat tcccgcgcgc acgtcgcgaa acggccgcga
      901 gtagccggct cggcgcagcg ccgtgatgtc ggcttccgtg aagctctgat agcgcgagcg
      961 cagctcgtcg gggaaaggga tgtagcgaat ggcgcccctg ccgtgccact cgatgatcgc
     1021 tttcgcgacc tcgttgaacg tcgcgctcga gcccgtgccg acgttgaaga tgccggatac
     1081 cttctcgtgc tcgaggagcc agacgttcac gtcggcgacg tcgccgacgt gaacgaaatc
     1141 ccgccgctgc tctcccggcc catagccgcc gcttccctcg aacagcttgg cctgacccgt
     1201 cgccgcgact tggccatgca agtggaacgc gacgctcgcc atcgcgccct tgtgcgcttc
     1261 cccgggtcca tagacgttga agtaacgcag cccgacgact tgcgctcgcc gtttcttgcc
     1321 gcgcccgcgc cgcccgcgga tgcgcctgcg cacgtactgg tcgaacagca gcttcgagta
     1381 gccgtacacg ttcaacggcc gctcggccgc gcggtcctct tcgctgaacg tcgtgctcgc
     1441 gccgtagacg gcggccgacg acgcatagat caacggcacg cccttgtcga gacaatgctc
     1501 gagcagctcg acggagtagc gatagttcgt ctccatcatg tagcggccgt tccattcggt
     1561 cgtcacggca cacgcgccct gatgaaagat cgcgtcgacc ttgcccaagt ctcgccgctt
     1621 cacgcgctcg agcagctcgg acttgtcgag atagtcggcg attcgacaat cgaccaagtt
     1681 gacgaacttg cggccgtcgc tcaaatcgtc gacgacgacg acgtcgtcgt ggccacgagc
     1741 gttgagctcg cggaccaagt tgctgccgat gaagccggcc ccgcaggtga cgacgatcat
     1801 ggcctcgttg agcggatcag cgcgtgtcgt cctcgaccaa gcgatacgtc gagctgcagt
     1861 aagggcattt cgcctcgccc gtggcctcta tcggcaaata gaccttcgga tgggagttcc
     1921 acagatactg gcttggcatc gggcaatgca gcggcaggtc cgcccgcgtg acctggtaca
     1981 cccgttcggc gttcgccggg atcaagttct cgttgatagt gccgtgacgt gggcgcaccg
     2041 c
//
LOCUS       DASSNN010000159         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_410162, whole genome shotgun sequence.
ACCESSION   DASSNN010000159 DASSNN010000000
VERSION     DASSNN010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410162"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFL84_02795"
     CDS             <1..220
                     /locus_tag="VFL84_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7607580.1"
                     /translation="AEPAEDLLALEGMDKTLALELASRGVVTREDLAEQAVDDLSEIA
                     GLDAERAAKLIMAARAHWFEQEQQAKNG"
     gene            232..>2536
                     /gene="infB"
                     /locus_tag="VFL84_02800"
     CDS             232..>2536
                     /gene="infB"
                     /locus_tag="VFL84_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET7607581.1"
                     /translation="MAEVTVADFAKVLKVPVDRLIEQLDEAGIKVGGPNDVISEDAKL
                     ELLTHLKKNHGRKDAAESAPRRITLSRKSQSELKLASSQGRARTINVEVRQKRTYIKR
                     DVLEEQARKQQEELDAVRRAQEEARNAVERSAREEQEKREAAERERLAAEQREAEERK
                     RLEEEQRRAAAADEAAKQRAEEERRKAAIAEKEAQQRADSERARQKAKKERMTDEPSE
                     GDHTLHVADGMAGRRKKKKQRRRAVPVNVDPQHAFEKPTAPVVREVEIGATITVSELA
                     ARMAVKANEVIKALMKMGMMVTINQVLDQDTATLVVEEMGHTAKPLKETAIEDQLIQS
                     VSEAASSEAAVPRAPVVTIMGHVDHGKTSLLDYIRRTKVAAGEAGGITQHIGAYNVET
                     PKGRITFLDTPGHAAFTAMRARGAKVTDIVVLVVAADDGVMPQTIEAIQHAKAAKVPI
                     VVAINKIDKPGGDLDRIRRELSNYELISEEWGGETLFVSVSALTGQGVDKLLEALLLQ
                     AELLDLKAVDSGPAAGVVLESSLERGRGAVATVLVNKGRLQVGDTLLAGEEYGRIRAM
                     FDENGKPITAAGPSTPVVVLGLSGTPSSGDDVQVVADERQARDVAAFRHSSKRETKLA
                     HQQASKLENIFSQMQAGGTPSIQLLIKADVHGSAEALRDALEKISSDDVKVTVVAAAV
                     GGITESDVQLAATSKAIIIGFNVRADAGARGAIKESGVDIRYYSIIYEAIDDIKAAAS
                     GLLAPEVREQIVGLAEVREVFRSPKFGQ"
BASE COUNT          505 a          829 c          905 g          297 t
ORIGIN      
        1 ggcggagccg gccgaggacc tgttggccct cgaaggcatg gacaagacgc tcgcgctcga
       61 gctcgcgagc cgcggcgtcg tgacgcgcga ggatctcgcc gagcaggcag tcgacgatct
      121 gtccgagatc gccggcctcg acgccgagcg ggccgcgaag ctgatcatgg ctgcgcgggc
      181 gcattggttc gagcaagagc agcaagcgaa gaacggctga ggtaggtacg catggcggaa
      241 gtcacggtcg cggatttcgc gaaggtactg aaggtcccgg tcgaccggct catcgagcag
      301 ctcgacgagg ccggcatcaa ggtcggcggg ccgaacgacg tcatcagcga ggacgcgaag
      361 ctcgagctgt tgacgcacct gaagaaaaac cacggccgta aagacgccgc ggagtccgcg
      421 ccgcgccgca tcacgctgag ccgcaagtcg cagagcgagc tcaagctcgc gagcagccag
      481 ggccgcgcgc gcacgatcaa cgtcgaggtg cgccagaagc gcacctacat caagcgcgac
      541 gtgctcgagg agcaggcccg caagcagcag gaagagctcg acgcagtgcg ccgcgcgcaa
      601 gaagaggcgc gcaatgccgt cgagcgctcg gcccgcgagg agcaagagaa gcgcgaggcg
      661 gccgagcgcg agcggctcgc ggcggagcag cgcgaggccg aggagcgcaa gcggctcgag
      721 gaagagcagc gccgcgcggc cgcggccgac gaagccgcga agcagcgcgc cgaagaggaa
      781 cgccgcaagg ccgcgatcgc cgagaaggag gcgcagcagc gcgccgactc cgagcgcgcg
      841 cgccagaagg ccaagaaaga gcgcatgacg gacgagccca gcgagggcga tcacacgctg
      901 catgtcgccg acggtatggc cgggcggcgc aagaagaaga agcagcgccg tcgcgccgtg
      961 ccggtcaacg tcgatccgca gcacgcgttc gagaagccga cggcgcccgt ggttcgcgag
     1021 gtcgagatcg gcgcgacgat cacggtcagc gagctcgcgg cgcgcatggc cgtcaaggcc
     1081 aacgaggtca tcaaagccct catgaagatg ggcatgatgg tcacgatcaa tcaggtgctc
     1141 gatcaggaca cggcgacgct cgtggtcgag gagatgggcc acaccgcgaa gccgctcaag
     1201 gaaacggcga tcgaggacca gctcatccag tccgtctcgg aagccgcgag ctccgaggcc
     1261 gccgtccccc gcgcgcccgt cgtcacgatc atgggccacg tcgaccacgg caagacgtcg
     1321 ctgctcgact acatccggcg cacgaaagtc gccgcgggcg aagcgggcgg aatcacgcag
     1381 cacatcggcg cgtacaacgt cgagacgccg aagggccgca tcacgttcct cgacacgccc
     1441 ggccatgcgg cgttcacggc aatgcgcgcg cgcggcgcca aggtcacgga catcgtcgtg
     1501 ctcgtcgtcg cggccgacga cggcgtcatg ccgcagacga tcgaggcgat tcagcacgcg
     1561 aaagccgcga aggtgccgat cgtcgtcgcc atcaacaaga tcgacaagcc cggcggcgat
     1621 ctcgatcgca ttcgccgcga gctctcgaac tacgagctga tctcggagga gtggggcggc
     1681 gagaccctgt tcgtcagcgt gtccgcgctg acggggcaag gcgtcgacaa gctgctcgaa
     1741 gcgctgttgc tgcaggccga gctgctcgac ttgaaggcag tcgactccgg cccggcggcc
     1801 ggcgtcgtgc tcgagtcgag cctggagcgc ggccgtggcg ccgtcgccac cgtgctcgtg
     1861 aacaagggca ggctgcaagt cggcgacacc ttgctcgccg gcgaagagta cggccgcatc
     1921 cgcgcgatgt tcgacgagaa cggcaagccg atcacggcgg ccggcccgtc gacgcccgtc
     1981 gtcgtgctgg gcctgtcggg aacgccgtcg tccggagacg acgtgcaagt cgtggcggac
     2041 gagcgccagg cgcgcgacgt cgcggcattt cgccacagca gcaagcgcga gaccaagctc
     2101 gcgcaccagc aggcgtcgaa gctcgaaaac atcttcagcc agatgcaggc cggcggtacg
     2161 ccgagcatcc agctgctcat caaggccgac gtgcacggca gcgccgaagc gctgcgcgac
     2221 gcgctcgaga aaatttcgtc ggacgacgtc aaggtcacgg tcgtcgcggc ggccgtcggc
     2281 ggcatcacgg agtccgacgt gcagctcgct gcgacgtcga aggcgatcat catcggcttc
     2341 aacgtccgcg ccgatgccgg agcgcgcggc gccatcaagg agagcggcgt cgacatccgc
     2401 tactacagca tcatttacga ggcgatcgac gacatcaaag ccgcggcgag cgggttgctc
     2461 gcgccggaag tgcgcgagca gatcgtcggc ctcgccgagg ttcgcgaggt gttccgctcg
     2521 ccgaagttcg gccaag
//
LOCUS       DASSNN010000160         3798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_159340, whole genome shotgun sequence.
ACCESSION   DASSNN010000160 DASSNN010000000
VERSION     DASSNN010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159340"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..2768
                     /locus_tag="VFL84_02805"
     CDS             294..2768
                     /locus_tag="VFL84_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7607582.1"
                     /translation="MRAWRFATRSFVRGLRAGRLTILMVALTVAVAAITSIGFFIDRV
                     RASVAQEAAGLLAADLRENGSDPLNPDDLKEAQRRGLRTAEVTSFPTVVVAGDASQLA
                     SLYAVSESYPLRGQVRLSDSVGATSSYVVAGAPPAGAVWVEDGLLARLNADVGARVSI
                     GAREFAVERVLEHRPDQSLGFDTFAPSLMMNLADLASTELIRPGSRATYSQLFAGDAA
                     QVAAFKDWFESTPERRLQTAGRSEERIDRAIDRAERFLSLASMIALLLAATAVAVSAR
                     RYAAQQVEGVALLKCLGASQALVLAATLLELALIGVATGIAGSALGYLAQLGLGSLVE
                     DLMELTLPAPGLTPGVSGVFVALAVLAGFASPAMLNLRTVPPLRVLHQDAAPKAVPTA
                     VSYGAAAAAVLGLLAWLVGTDEMFVVSVVGLGLGAVLLYAAAHLLVRLIEPLRSGAGV
                     AWRYGLANIARRRADSVAQVMAFGFGLTVLLLLSGVRTSLLDTWYASLPTDAPNNFMI
                     NIQPEEADGVRALFEEYGQEAPKLVPMVRGRLTQIDGEDVMTRRMRGEGGWLFREANL
                     TWAEELDATNEIVAGEWWAPGTEGAEISVEEGIMQGLGLTLGDELTFEVGGFPLTATL
                     TSVRRVEWDSLSPNFMLVLNPAALKDYPATFVASTYAPNRDVMLELVRRFPSITVIDL
                     ASVLEQVRSIMDRAALAIQYVFLFTLLAGVVVLLAAVQATREERRFEVALLRTLGASR
                     RRVLAALVVEFASLGVLAGALAALIASGVAYWLATRVFQLDFTFDPIVVGAGLVAGGV
                     LVGLSGTLATYSVVRQPPAGVLAQAS"
     gene            complement(2815..2898)
                     /locus_tag="VFL84_02810"
                     /pseudo
     CDS             complement(2815..2898)
                     /locus_tag="VFL84_02810"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
     gene            complement(3422..>3798)
                     /locus_tag="VFL84_02815"
     CDS             complement(3422..>3798)
                     /locus_tag="VFL84_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607583.1"
                     /translation="GTVVRYRDIEILALSDELDPGELDLEELDDADEESGDDALELSE
                     LPEVRGHLGEMIARHYADWEMQELDVFGGGRPIDVMQRTNGPEKVAAVIEEMERHARD
                     GGPEGTVEALARLRERLGLVRR"
BASE COUNT          559 a         1285 c         1288 g          666 t
ORIGIN      
        1 tcaaacgagc gagctcttct ttcgctgttg acacgcgggc acctctaggc ctgtttgctc
       61 gcgttggcat tctgcactcg tggattgtac tcggctggcc caccgtcgca cgacagttgc
      121 agcctcagcc agcgacgaag ctcacgaaac tacgaagaaa cgagtgcggt ggccgacttg
      181 atgtcgcgaa atgcgacttc aagtccgcat cattcctagt ctgatcgaac ttcagcgaat
      241 tgcccgctcc gcgtagactg gcgttcgtcg cgaaacacgc ggagatccgt tcgatgagag
      301 cctggcgctt cgccacccgc agtttcgtcc gcggtcttcg cgcggggcgg ctgacgatcc
      361 tcatggtcgc gctgaccgtc gcggtcgccg cgatcacgag catcggcttc ttcatcgacc
      421 gcgtgcgcgc gagcgtcgcg caggaagcgg ccggtctcct cgccgcggat ctgcgcgaga
      481 acggcagcga tccgctgaat cccgacgacc ttaaggaagc gcagcggcgc ggcttacgca
      541 ccgcggaggt cacgtcgttc ccgaccgtcg tcgtcgcggg tgacgcgagc cagctcgcga
      601 gcctctacgc cgtcagcgag agctatccgc tgcgcggcca ggtgcggctc agcgattcgg
      661 tcggcgcgac gagcagctac gtcgtggccg gcgcgccgcc cgcgggcgcc gtgtgggtcg
      721 aggacgggct gctcgcgcgg ctcaatgccg acgtcggcgc gcgcgtctcg atcggcgcgc
      781 gcgaattcgc cgtcgagcgc gtgctcgagc accggccgga tcagtcgctc ggcttcgata
      841 cgtttgcgcc ttcattgatg atgaatctcg cggacctcgc gagcacggag ctgatccggc
      901 ccggcagccg cgcgacctac tcgcagctct tcgccggcga cgcggcgcaa gtcgcggcgt
      961 tcaaggactg gttcgagtcg actcccgagc ggcgcctgca aacggccggc cgctccgagg
     1021 aacgcatcga ccgcgcgatc gaccgcgccg agcgcttctt gagcttggcg agcatgatcg
     1081 cgctgctcct cgcggcgacg gccgtcgcgg tctcggcgcg ccgctatgcg gcgcagcaag
     1141 tcgaaggcgt cgcactcctc aaatgtctcg gcgcgtcgca agcgctcgtc ctcgccgcca
     1201 cgctgctcga gctcgcgctg atcggcgtcg cgaccggcat cgccggcagc gcgctcggct
     1261 acctcgcgca gctcggcctg ggctcgctcg tcgaggatct catggagctt acgctgccgg
     1321 cgccgggcct gacgcccggc gtcagcggcg tgttcgtcgc gctcgccgtg ctcgcgggat
     1381 ttgctagccc cgcgatgctg aacctgcgca ccgtgccgcc gctgcgcgtg ctgcaccagg
     1441 acgcggcgcc gaaggcagtg ccgaccgccg tgtcgtacgg cgccgcggcc gccgccgtgc
     1501 tcggcctgct cgcgtggctc gtcggcacgg acgagatgtt cgtcgtgtcc gtcgtcggac
     1561 tcgggctcgg cgccgtgctg ctctatgcgg ctgcgcacct gctcgtccgg ctcatcgagc
     1621 cgctccgcag cggcgccggc gtcgcgtggc gctacgggct cgcgaacatc gcgcgccgcc
     1681 gcgccgatag cgtcgcgcaa gtcatggcgt tcggcttcgg gctcacggtg ctgctgttgc
     1741 tctcgggcgt gcggacgagc ttgctcgaca cgtggtacgc gagcctgccg accgacgcgc
     1801 cgaacaactt catgatcaac atccagcccg aggaagccga cggcgtccgc gcgctgttcg
     1861 aggagtacgg ccaggaggcg ccgaagctcg tgccgatggt gcgcggccgc ctgacgcaga
     1921 tcgacggcga ggacgtcatg acgcggcgca tgcgcggcga aggcggctgg ctgttccgcg
     1981 aggcaaacct gacctgggcc gaggagctcg acgcgacgaa cgagatcgtc gccggcgagt
     2041 ggtgggcgcc cggtacggag ggcgccgaaa tctccgtcga agaaggcatc atgcaggggc
     2101 ttgggcttac gctcggcgac gagctgacgt tcgaggtcgg cggcttcccg ttgacggcga
     2161 cgctgacgag cgtgcggcgg gtcgagtggg actcgctctc gccgaacttc atgctcgtgc
     2221 tgaatcccgc cgccttgaag gattaccccg cgacgttcgt cgcttcgacg tatgcgccga
     2281 accgcgacgt gatgctcgag ctggtgcggc gcttcccttc gatcacggtc atcgatctcg
     2341 ccagcgtgct cgagcaggtg cgcagcatca tggaccgcgc cgcgctcgcg attcagtacg
     2401 tgttcctgtt cacgctgctc gcgggcgtcg tcgtgctgct cgcggccgtg caggcgacgc
     2461 gcgaggagcg ccgcttcgag gtcgcgctgc tgcgcacgct cggcgcgtca cggcgccgcg
     2521 tgctcgccgc gctcgtcgtc gagttcgcga gcttgggcgt gctcgcggga gcgctcgcgg
     2581 cgttgatcgc gtcgggcgtc gcgtattggc tcgcaacgcg cgtgtttcag ctcgacttta
     2641 cgttcgatcc gatcgtcgtc ggcgcggggc tcgtcgcggg tggggtgctc gtgggattga
     2701 gcgggacgct cgcgacgtac tcggtcgtgc ggcagccgcc ggcgggcgtg ctcgcgcagg
     2761 cgagctagcg gcacatccaa tctcggcgga ttcgactacc acggatccga cgaccgtcgc
     2821 gaccggaatg cgtaggccgc gaaaccgcgg cacgccgccc atttgattct gattcaccgt
     2881 gacgcgcgtg aacttcattt cgcacgtcca aaaccgtcag cgttcgatga taagccccgg
     2941 caaaagcgcg ctgtcaagcg tgtaagaggc caatacatcc tgcacgggac tgtcagccgc
     3001 cggcgagcgc gttggcgcgg gagtagttaa tccgtatttg acgaaggcga gagacggcgt
     3061 gcggcgtgaa gacgaccttc acgtcctaag gcgaagctga cgacgcacaa cggttgcgtc
     3121 ggagactcgc ccgctttcag catcagcgag accgagctcg atcgaccggc gagccgcgag
     3181 ctcgtacacg atgtcatccc acgtgacatc gtccgggagg ttctccagca aagcacgcgc
     3241 ttgttctttc gtagtaggac tatgcgtggc catcaatcga cctccgaaca atttgggtgg
     3301 caatctactc ttcggaagca gcgatcagca accgacgaat ctgtcctttt cgcagcgatt
     3361 gtcgtcgcca agggtttgca cgaaacacca cgcgcgagcg ggaggcgatt gagcaaggcc
     3421 gctagcgccg cacgaggccg agccgctcgc gcaagcgcgc gagcgcttcg accgtgcctt
     3481 cgggcccgcc gtcgcgcgcg tggcgctcca tttcctcgat gaccgctgcc actttctcgg
     3541 ggccgttcgt tcgctgcatc acgtcgatcg gccggccgcc gccgaacacg tccagctctt
     3601 gcatctccca gtcggcgtag tggcgcgcga tcatctcgcc gaggtgcccg cggacttccg
     3661 gcaactccga gagctcgagc gcgtcgtcac cgctctcctc atcggcatca tcgagctctt
     3721 cgaggtcgag ctcgccgggg tcgagctcgt cgctgagcgc gaggatctcg atgtcgcgat
     3781 agcgcacgac ggtgccgg
//
LOCUS       DASSNN010000161         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_159440, whole genome shotgun sequence.
ACCESSION   DASSNN010000161 DASSNN010000000
VERSION     DASSNN010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159440"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VFL84_02820"
     CDS             complement(<1..1434)
                     /locus_tag="VFL84_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HET7607584.1"
                     /translation="MTTVAPAQIALSLISHTNVGKTALARTLLGRDVGEVRDEAHVTA
                     TADRFPMIESPAGDVLALWDTPGFGDSARLARRLATLDKPILGFLAMTWDRFRDRALW
                     SSQQAVRNVRDEADVVLYLVNAAEMPADAGYVEPELTILAWIGKPVIVLLNQTGPPRG
                     AAQEAAETARWRDALGPRPLIRSVLPLDAFARCWVQEGTLLREVAKALPADKQAAFAR
                     LAAAWQQKRRSQFDDAMAAIAEPIARAACDRVAVPQAGLRGSIVEVGRAIGLGSPRDD
                     TVKAAAMRSLSERLDADIRASTDRLIALHDLDGRAAAEVTARLLDNVTAETPVHEGKA
                     AVIGGFVSGALTGLAADLASGGLTFGAGLLAGGVVGAVGAAGLARGFNLVRGRTGTTI
                     RWSDEFVATLVPAAILRYLAVAHYGRGRGEWAASEYPPFWRDEVVAAIAREGGVAAEL
                     ALRADEACNPGMLAVAWRAKLGAIALAT"
     gene            complement(1431..>2123)
                     /locus_tag="VFL84_02825"
     CDS             complement(1431..>2123)
                     /locus_tag="VFL84_02825"
                     /inference="COORDINATES: protein motif:HMM:NF022518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HET7607585.1"
                     /translation="AVPGAAHLQTVGAGSAGENAAPWIHLYAATLLLAVIVPRLVLAG
                     FAWAAERRQQSRFPLALAEPYFQRLLHAWREGTARVAAVAYSYAIAKPNVDALARLLT
                     RAFESAVDVQWLPPAAYGEDDVPTLPPPPLAAVVIVFNVNATPERENHGAFASALRAA
                     LAGRAPLAAIVDTSDFDLRFGATSRADERRHAWEQVLGDANVAPLFVRLADPDVATAA
                     ATLTTRFSALPA"
BASE COUNT          302 a          800 c          749 g          272 t
ORIGIN      
        1 cgtcgcaagc gcgatcgcgc cgagcttcgc gcgccaggcg acggcgagca tcccggggtt
       61 gcacgcctcg tccgcgcgca gggccagctc ggccgcgacg ccgccttcgc gcgcgatcgc
      121 ggcgacgacc tcgtcgcgcc agaacggcgg atattcgctg gctgcccact cgccgcggcc
      181 gcgtccgtag tgcgcgacgg ccagatagcg caggatcgcg gcgggaacga gcgtcgcgac
      241 gaactcgtcg ctccagcgga tcgtcgtgcc ggtgcggcca cgcacgaggt tgaagccgcg
      301 cgcgagcccg gcggcgccga cggccccgac gacgccgccc gccagcagcc ccgcgccgaa
      361 cgtcagcccg cccgatgcga gatcggcggc gagaccggta agcgcacccg atacgaatcc
      421 gccgatgacc gcggccttgc cttcgtgaac cggcgtctcg gcggtcacgt tgtcgagcag
      481 gcgcgcggtc acttccgcgg cggcgcgtcc gtcaagatcg tgaagtgcga tcagcctatc
      541 ggtgctggcg cggatgtccg catcgagccg ctcggaaagc gagcgcatcg ccgccgcctt
      601 caccgtatcg tcgcgcgggg accccaaccc gatcgccctg ccgacttcga cgatcgaacc
      661 gcgcagcccg gcctgcggga cggccacgcg atcgcacgcc gcgcgcgcga tcggttccgc
      721 gatcgccgcc atcgcgtcgt cgaactgcga gcgccgcttc tgctgccagg ccgccgcgag
      781 tcgcgcgaag gccgcctgct tgtccgccgg cagcgccttc gcgacttcgc gcagcagcgt
      841 accctcctgc acccagcagc gcgcgaatgc gtcgagcggc agcacgctgc ggatcagcgg
      901 ccgcgggccc aatgcgtcgc gccagcgcgc cgtctccgcg gcctcctgcg ccgcgccgcg
      961 cggtgggccg gtctgattga gcagcacgat caccggcttg ccgatccacg cgaggatcgt
     1021 gagctccggc tcgacgtagc cggcatcggc cggcatttcc gccgcattca cgagatacag
     1081 cacgacgtcg gcctcgtcgc gcacgttgcg caccgcctgc tggctcgacc agagcgcgcg
     1141 gtcgcgaaag cgatcccacg tcatcgcgag aaagccgagg atcggcttgt cgagcgtcgc
     1201 gaggcggcgg gcgagccttg cgctgtcgcc gaagcccggc gtgtcccaga gcgcaagcac
     1261 gtcgcccgcc ggcgattcga tcatcggaaa gcggtccgct gtcgcggtca cgtgcgcctc
     1321 gtcgcgcacc tcgccgacgt cgcggccgag cagcgtgcgc gcgagcgccg tcttgccgac
     1381 gttggtgtgc gagatcagcg agagcgcgat ctgcgcaggc gcgaccgtcg tcatgccggc
     1441 aacgccgaga agcgtgtcgt cagcgtggcc gctgccgtcg cgacgtccgg atcggcgagc
     1501 cgcacgaaca gcggcgcgac gttcgcgtcg ccgagcacct gttcccatgc gtgccgccgc
     1561 tcgtccgccc gcgacgtggc gccgaagcgc aggtcgaagt ccgacgtgtc gacgatcgcg
     1621 gcgagcggcg cgcggccggc gagcgcggcg cgcaacgcgc tcgcgaaggc gccgtgattc
     1681 tcgcgctcgg gcgtcgcatt gacgttgaac acgatgacca cggcggcgag cggtggcggc
     1741 ggcagcgtcg gcacatcgtc ttcgccatac gcggccggtg gcagccactg cacgtcgacc
     1801 gccgactcga acgcgcgcgt cagaagccgc gcgagcgcat cgacgttcgg cttggcgatt
     1861 gcgtagctgt acgcgaccgc tgcgacacgc gccgtcccct cgcgccacgc gtgcagcaac
     1921 cgctggaaat acggctcggc gagcgcgagc gggaagcgcg attgctgccg ccgctccgcc
     1981 gcccacgcga acccggcgag cacgagccgc ggcacgatca ccgcgagcag cagcgtcgcg
     2041 gcgtagaggt gaatccacgg cgccgcgttc tcgcccgcgc tgcccgcgcc gaccgtttgc
     2101 agatgcgcgg cgccgggcac cgc
//
LOCUS       DASSNN010000162         5943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_32214, whole genome shotgun sequence.
ACCESSION   DASSNN010000162 DASSNN010000000
VERSION     DASSNN010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32214"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFL84_02830"
     CDS             <1..214
                     /locus_tag="VFL84_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607586.1"
                     /translation="TLRSEYPRLEGELRPLLVSLALFSLLTLAAGSSFYGMLKERPWR
                     RIALTALVAILAAVAAYHAWPVITRG"
     gene            complement(211..504)
                     /locus_tag="VFL84_02835"
     CDS             complement(211..504)
                     /locus_tag="VFL84_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607587.1"
                     /translation="MLKQLKRWLGWEGESDLLDLAGAGPRATAPPAPKPAITVQTRPA
                     QPRPAKPEKTPLDVLDDPRLTLDKPADDGFDPYNTGAFNRSASWERIGRQRKR"
     gene            complement(618..911)
                     /locus_tag="VFL84_02840"
     CDS             complement(618..911)
                     /locus_tag="VFL84_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607588.1"
                     /translation="MADDMDTPVAGAINWRREVSLAAGLLAFGLLALPFAVYVVGQRL
                     LGEYGDGLGPFALAETIWLDLLSLRLPAWVLVLSPYLTIQLARGVRRIWRRKV"
     gene            complement(917..2602)
                     /locus_tag="VFL84_02845"
     CDS             complement(917..2602)
                     /locus_tag="VFL84_02845"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7607589.1"
                     /translation="MSKRSSDDFDHDDTDELPVLLDPVGLEDAELLESAAQPSLPPAP
                     SVEAAAQPLAPERDAASSQALLSDAARPDEQIPALEAQIGVLTDSMRDLENRLIEKDR
                     RLEELREMLSAVRRSADDSGATQKRLATQLAVRDARIAELTATIERLEQAATVATAEI
                     ERQRQLAEARNSEAEALRRELETQPEPQPAAAPAPAPAPAGPDEQLTENYATLKSYVA
                     ARRAWWDEMQESNARLAARVTGLEEELAASAKGLAAAEAFASRESSRAVGLRAELVDY
                     ARRVDELERQLRLLRAQPEAANGAGADEHALDAPTASAPSATAADAGFGDASRQPPPG
                     IIEAVEAISPAVEAIAQLEAEVEYKRQQVAAQLVELHARELQLRAATSAIGELRGELD
                     ASRSNVARLEQAVVDKDRALEARDARIAALHEELKQRIAAAERRTAADLAPPALANAG
                     RRDGLHEAAADSASTPALICLTGDAPKRFALTKSTVTVGRGPHCDLQIVTHFVSREHA
                     RLTLSGGATLIEDLGSRNGVFVNSVRVDRRRLQQGDLVTIGETQFRFVESMAH"
     gene            complement(2672..4156)
                     /locus_tag="VFL84_02850"
     CDS             complement(2672..4156)
                     /locus_tag="VFL84_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7607590.1"
                     /translation="MNDRRDLSLVLESGTPLIVIETTDETRVLELLREIALARSASSY
                     RPLFRWSITDGIQRLDLELEAQRHNADPEEALRHIRATGKPGVFALLDFHPFLKEPVH
                     IRLLKDIALAADSAKITLLLIGHRLEIPQELMGFTARFSLRLPDVNDRRAIVQRCADD
                     YLNETGRRVKVDPAALELLVRNLSGLTTADTERLARNAIRDDGAVTSSDLPAVMKAKY
                     QLLNRGGVLCFEYDTTRLSELAGFRNLKQWLAQRTWAFRPDPPAGLEPPKGILLLGVQ
                     GCGKSLAAKATAGAFGVPLLRLDFGSLYNKFHGETERNLRESLQTAEVLSPCVLWLDE
                     IEKGLATGNDDSGTARRILGGLLTWMAERRAQVFMVATANEVSELPPELIRKGRFDEI
                     FFVDLPPPPSRAEILTIHLRKRGLAPERMNLAELVALTDGFSGAEIEQGIVSALYAAH
                     ALEQAPEQAHVAAELRKTRPLSVIMAERVAALRAWASERTVPAE"
     gene            complement(4275..4994)
                     /locus_tag="VFL84_02855"
     CDS             complement(4275..4994)
                     /locus_tag="VFL84_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607591.1"
                     /translation="MKPLRLLGIAAALPLIGACGVTARLEPYKQTDAVIAKGEHVVVL
                     ARKQHSTHEAEEEFVQCISDGLADGKHGLEVHSSKDFEDKMYPWFEPSTAPLSTDDLT
                     VLLARPGVAEQVRETGVRYVVWVDGSTDRVASGGGITCAAGVGGAGCMGLAWWEDDSR
                     YDLTVWDVKDIENEGTIYADYKGRSVMPAIVIPIPLITRPQTHACRGLTDQLRQFLTT
                     PEGYSSPGAPPPVPAATADVG"
     gene            complement(4996..5694)
                     /locus_tag="VFL84_02860"
     CDS             complement(4996..5694)
                     /locus_tag="VFL84_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607592.1"
                     /translation="MSLLPLDRSRLRLLAVATTACLAGCGGSQVIVESTFPRPLVEQL
                     PVSMGVVIPDELYNFIYTEDIPDQSLWTIALGDANVAMLAPLFEGMFEQTRDLDSLAL
                     AAGDRTLDGVIEPKLEKFEFDVPQGERDKFVEVWLQYEITVYEPNGSTVIEWPVSGYG
                     KSELLRDAEDAVQRAAVVAMREAGATIATKFSEQPQIKEWLGGIANAAPTTVGPQAAV
                     DGNATAESAGAPLE"
BASE COUNT          894 a         1983 c         2035 g         1031 t
ORIGIN      
        1 gacgctacgg tccgagtatc cgcggctcga gggcgagctc cgcccgttgc tcgtcagcct
       61 ggccttgttc tcgctgctca cgctcgcggc gggcagcagc ttctacggca tgctgaagga
      121 gcgcccgtgg cggcgcatcg cgttgacggc gctggtggca attctagcgg ccgtggcggc
      181 gtatcacgcc tggcccgtga tcacacgcgg ctagcgcttg cgctggcggc cgatccgttc
      241 ccacgaagcg ctgcggttga atgcgcctgt gttgtaggga tcgaagccgt cgtcggcggg
      301 cttgtcgaga gtcagacgag ggtcgtcgag cacgtcgagc ggcgtttttt cgggcttcgc
      361 aggccgaggt tgcgcggggc gtgtttgcac cgtgatcgcc ggcttcggtg cgggcggcgc
      421 agtcgcgcgc gggccggcgc cggcgagatc cagcaaatcg gattcgcctt cccaaccgag
      481 ccaacgtttc aactgcttca gcattggcga tcgattcccg ttatgccaaa tacgcggaac
      541 gcgctagccc gcgttttgga aaacataaca gaagctctcg gcgtctctag tgacgtgtgc
      601 cgaatttgtg acggcactca caccttgcgc cgccaaatgc gacgcacgcc acgcgcgagc
      661 tgaatcgtga ggtaggggct cagcacgaga acccacgccg ggaggcgcag cgacagcaag
      721 tcgagccaaa tggtttcggc gagagcgaag ggtcccaagc cgtcgccata ctcgccgagc
      781 aagcgttggc cgacgacata cacggcgaac ggcagcgcaa gcagaccgaa cgcgagcagc
      841 cccgccgcga gcgagacttc gcgccgccaa tttatcgcgc cggcaaccgg cgtatccatg
      901 tcatccgcca cggccattag tgtgccatcg attcgacgaa gcgaaactgc gtctcgccga
      961 tcgtgacgag atcgccttgc tggaggcgcc gacggtccac gcgcacggaa ttgacgaata
     1021 cgccgttgcg gctgccgagg tcctcgatca gcgttgcgcc gccgctcaga gtaaggcgag
     1081 cgtgctcgcg gctcacgaag tgcgtcacga tttgcagatc gcagtgcggt ccgcggccga
     1141 cggtgacggt gctcttcgtc agagcaaatc gcttcggtgc gtctccggtc agacagatca
     1201 gcgccggcgt cgaggcgctg tcggcggccg cttcatgcaa gccgtctcgg cgccccgcgt
     1261 tggcgagcgc cggcggcgcg aggtcggcgg cggtgcgtct ctcggccgcg gcaatgcgct
     1321 gcttgagctc ctcgtgcagc gcggcgattc tcgcgtcgcg cgcttcgagg gctctatcct
     1381 tgtcgacgac cgcttgctcg agtcgagcga cgttcgagcg gctggcgtcg agctcgccgc
     1441 gcagctcgcc gatcgcgctc gtcgccgcac gcagttgcag ctcgcgcgcg tggagctcga
     1501 cgagctgcgc ggccacctgc tgccgcttgt attcgacttc cgcctcgagc tgggcaatgg
     1561 cctcgacggc gggcgagatc gcctcgacgg cttcgatgat ccccggcggt ggctgtcgcg
     1621 aagcgtcgcc gaaaccggcg tcggccgccg tcgcgctcgg cgccgaagcc gtcggcgcgt
     1681 cgagcgcgtg ctcgtcggcg cccgcgccgt tcgcggcctc cggttgcgcg cgcagcagcc
     1741 gcagctgccg ctcgagctcg tcgacgcgcc gagcgtagtc gacgagctcg gcgcgaagcc
     1801 ccacggcgcg gctcgattcg cgagacgcaa aggcctcggc cgctgcgagc cccttcgcgc
     1861 tggccgcgag ctcttcctcg agcccggtca cgcgcgccgc aagccgcgcg ttcgactctt
     1921 gcatttcatc ccaccacgcg cgccgggccg cgacgtagga cttcagcgtc gcatagtttt
     1981 cggttagctg ttcgtcgggc cccgccggtg ccggtgccgg cgccggcgcc gccgccggct
     2041 gcggctcggg ttgcgtctcg agctcgcgcc gcagcgcttc cgcttcgctg ttgcgcgcct
     2101 ccgcgagctg gcgctggcgc tcgatctccg ccgttgccac ggttgccgct tgctcgaggc
     2161 gctcgatcgt cgccgtcagc tccgcgatcc gcgcatcgcg gaccgcgagc tgcgtggcga
     2221 ggcgtttctg cgttgcgccc gagtcgtccg ccgagcggcg aaccgccgac agcatttcgc
     2281 gcagctcttc gagacggcga tctttttcga tcaagcggtt ctcgagatcg cgcatgctgt
     2341 cggtcaacac gccaatctgc gcctcgagcg ccggaatttg ctcgtcgggc cgcgcggcat
     2401 cggacaggag cgcctgcgag ctcgccgcgt cgcgctcggg cgcgagcggc tgcgccgcgg
     2461 cctcgacgga cggcgcaggg ggcaacgacg gctgcgcggc gctctcgagc agctccgcgt
     2521 cctcgaggcc gacgggatcg agcagcacgg gcagctcgtc ggtgtcgtcg tggtcgaaat
     2581 cgtccgagga tcgttttgac atcgaggcag ttacgcgcct ttcttctgct attacgttag
     2641 gccgatttcg ggcggcccgc cagcgctgcc gttattcggc cgggaccgtg cgctcgctcg
     2701 cccaggctcg cagcgcggcg acgcgctccg ccatgatgac cgacagcggc cgggtcttgc
     2761 ggagctcggc ggcgacgtgg gcttgctcgg gggcctgctc cagcgcgtgc gcggcgtaca
     2821 gcgccgatac gatgccttgc tcgatctccg cccccgagaa gccgtcggtc agagccacga
     2881 gctccgcgag gttcatgcgc tcgggagcga gcccgcgctt gcgcaaatga atcgtcagga
     2941 tctccgcgcg gctcggcggc ggcggcaagt cgacgaagaa gatttcgtca aaccgcccct
     3001 tacggatcag ctccggcggc agctcgctga cctcgttggc cgtggcgacc atgaagacct
     3061 gcgcgcgccg ttctgccatc cacgtgagca ggccgccgag gatccggcgc gcggtcccgc
     3121 tgtcgtcgtt tcccgtcgcc aggccttttt cgatctcgtc cagccatagc acgcacgggc
     3181 tcagcacttc cgctgtttgc agcgattcgc ggaggtttcg ctccgtctcg ccgtggaact
     3241 tgttgtatag cgagccgaag tccaagcgca gcagcggcac gccgaacgct ccggccgtag
     3301 ccttcgccgc gaggctcttg ccgcagccct gaacgccgag cagaaggatg cccttcggcg
     3361 gctcgaggcc cgccggcgga tcggggcgga aagcccaggt tcgctgcgcc agccactgct
     3421 tgagattgcg gaatcccgcg agctcggaca gccgcgtcgt gtcgtactcg aagcagagca
     3481 cgccgccgcg gttgagaagc tggtacttcg ctttcatgac ggccggcagg tcgctcgacg
     3541 tgacggcccc gtcgtcgcga atcgcattgc gcgcgagccg ctccgtgtcc gccgtcgtca
     3601 agccgctcaa gttccgaacc aggagctcga gcgcggcggg gtccactttg acgcgacggc
     3661 cggtctcgtt cagatagtcg tcggcgcagc gctgaacgat cgcgcgccga tcgttcacgt
     3721 ccggcaagcg cagagagaag cgcgccgtga agcccatgag ctcctgcgga atctcgaggc
     3781 gatggccgat caggagcagc gtgatcttgg cgctgtccgc cgccagtgcg atgtctttga
     3841 gcaaccgaat gtgaaccggc tcttttaaga acgggtggaa atcgagcaat gcgaacacgc
     3901 ccggcttgcc ggttgcgcgg atgtggcgca gcgcctcctc cgggtcggcg ttgtggcgct
     3961 gcgcctcgag ctcgagatcg agccgctgga tcccgtccgt gatcgaccag cggaagaggg
     4021 ggcggtacga gctcgcggag cgggcgagcg cgatctcgcg caacagctcg agcacgcgcg
     4081 tctcgtccgt ggtctcgatg acgatcagcg gcgttcccga ttcgagaacg agcgagaggt
     4141 cgcggcggtc gttcatcaat gacggcttgc ggtggcgcag ccggtccgga tgcccaatgc
     4201 agcggccagt gtatgccggc agcggcgctg cggcggcgac ggagtcggca gatttccggc
     4261 cgagagaggc ggcgctagcc gacgtcggcc gtcgcggccg gcacaggcgg cggtgcgccg
     4321 ggcgacgagt agccttccgg tgtcgtcaag aattgccgca gctggtcggt caacccgcgg
     4381 cacgcgtgcg tttgcggccg tgtgatcagc gggatcggga tcacgatcgc aggcatgacg
     4441 gagcggccct tgtagtcggc gtagatcgtg ccctcgttct cgatgtcctt gacgtcccag
     4501 accgtcaagt cgtaacgcga gtcgtcttcc caccaagcca ggcccatgca acccgcgccg
     4561 ccgacgccgg ccgcgcacgt gatgccgccg ccgctcgcga cgcggtccgt ggatccgtcg
     4621 acccagacga cgtaccggac gcccgtttcg cggacctgct cggcgacacc ggggcgtgcc
     4681 agcagcaccg tgaggtcgtc ggtggagagc ggcgcggtcg aaggctcgaa ccacgggtac
     4741 atcttgtcct cgaagtcctt cgacgagtgc acctcgaggc cgtgcttgcc gtcggccagg
     4801 ccgtcgctga tgcactgaac gaattcctct tcggcttcgt gcgtcgagtg ctgcttgcgt
     4861 gcgaggacca cgacgtgctc gcctttggcg atcacggcgt cggtttgctt gtacggctcg
     4921 agccgcgcgg tgacgccgca ggcgccgatg agcggcaatg cggccgcgat accgaggagg
     4981 cgcaggggct tcatgctatt caagcggtgc gcccgccgat tcggccgtcg cgtttccgtc
     5041 gacggctgcc tgtggcccga ctgtggtggg tgcggcattg gcgatacccc ctaaccattc
     5101 cttgatttgc ggttgctcac tgaacttcgt tgcaattgtc gcgccggctt cgcgcatcgc
     5161 gacgacggcg gcgcgttgca ccgcgtcctc ggcatcgcgc aacagctcgg acttgccgta
     5221 acccgatacg ggccattcga tgacggtcga gccgttcggc tcgtacaccg tgatctcgta
     5281 ctgcagccag acctcgacga acttgtcgcg ctcgccctgc ggcacgtcga actcgaactt
     5341 ctcgagcttc ggctcgatga cgccgtccaa ggtccggtcg ccggccgcga gcgccagcga
     5401 gtcgagatcc cgggtctgct cgaacatgcc ctcgaacagc ggcgcgagca tcgcgacgtt
     5461 cgcgtcgccg agcgcgatcg tccagagcga ctggtcgggg atgtcttcgg tatagatgaa
     5521 gttatagagc tcgtcgggaa tgacgacgcc catgctgacg ggaagctgct cgacgagcgg
     5581 gcgcgggaac gtcgattcga cgatgacttg agagccgccg caacccgcga ggcacgcggt
     5641 cgtggcgacc gccagcaggc ggagcctcga ccggtctaag ggcagcaggg acatgagccg
     5701 atcttacgtc ctgtgcagat gaaatacgcc ccccgattga acgttaacac aggcggcgag
     5761 cgtggaacaa agcgactgtt agctctgacc ccgggcctgc aaaaagattc gacgcgccga
     5821 ttgaacgttc gaggaacgtg acgggacggc ggcgccggtc ggcgcctcgg gcaggccgtc
     5881 aggcgccgtt cagtcctcgt cgagtagaat tctttcatga atatgcaatc ggcccggatt
     5941 cgg
//
LOCUS       DASSNN010000163         4852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_537209, whole genome shotgun sequence.
ACCESSION   DASSNN010000163 DASSNN010000000
VERSION     DASSNN010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537209"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1838)
                     /locus_tag="VFL84_02865"
     CDS             complement(<1..1838)
                     /locus_tag="VFL84_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607593.1"
                     /translation="MASRPRMLCTGVAVAATLVLGVPSVASAQEEGEAFGPQYSTAPP
                     TGAGRRGGGGGGGPGGPGGPEYTPAADAKDLKSVLFNWAWHMGMLRSTEEYDLLMSLM
                     YQGKGTVQVGGQPCNVTMYRSDISYQTSGERIRITGTRPNGQSCNTIEVLSGAYSWNE
                     DIMGAELVAGEGKATPMPAAVEERMIRLWANPQGAWKAAMFGTQDTDTPQMAARPQRV
                     PADVMKVHKTSVAWQGKTPVVTFPIPGVPTATATATLDAKFMASSVVVKNGADTYEFT
                     YGNYKDWNNPLHPAEAFYTGRITEKKNGTVMRDITTTMTETGQLYVVMPVPASVKAAI
                     KPTHEPANWTLTPRNPPTPVAAAPTPRRADGKPDLSGNWNRPLNPVSGSGNRRCGPTQ
                     VKGGGINPEYGCTGGGDNFWIEYAWISPSRFGILGRPNYKPELWDHIQELDMWTNKYD
                     PVMTCRPLGLPRHGTPTRIVQTDKDVIFFYPANADYGGGNNEYRDIPIDGRKRSTLDE
                     SLTTFYGQSLGTWEGDTLVIDSINFDDSTWLGRGGLIHSASMHVVERLTRVGNEIRYD
                     MTIEDPEMFLEPWVMPTRVLTLAQGPGADRIPAERANCEVYEENNVT"
     gene            complement(1860..2255)
                     /locus_tag="VFL84_02870"
     CDS             complement(1860..2255)
                     /locus_tag="VFL84_02870"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7607594.1"
                     /translation="MIKQALGSVVVFGSLVASGSLQAHHSTAGVYDPGKEVKVTGALA
                     KLQFVNPHGSIVVTVKNADGTTTDWTFTTGSATALASQGITKVGPNALKIGEEVTVVG
                     TPARNGSPLGGLRSLTRADGTVLGNAGGN"
     gene            complement(2426..2854)
                     /locus_tag="VFL84_02875"
     CDS             complement(2426..2854)
                     /locus_tag="VFL84_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607595.1"
                     /translation="MITAALFLAILIDPNDDDVLIVWDYVVGFVGVVASVVLAGALLV
                     MRRAQLARAGLWRLASGSSAPPYRAARGRRKTRAPPRGGDRGGGVDRRVGHLLHCRPN
                     QRRSARRKRWVAVADLDRVRVHLPVVVQVGAPLTPAKGGS"
     gene            complement(2851..3342)
                     /locus_tag="VFL84_02880"
     CDS             complement(2851..3342)
                     /locus_tag="VFL84_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7607596.1"
                     /translation="MLDSERDRIFGDWLAAHRGILFKVVRAYAFKHADRQDLFQEIAI
                     QVWRSVDSYRGDSSVPTWMYRIALNAAISWTRKQGRHQRGKQPLEIVEGLLTVSGAEP
                     DPRVDWLYRRIAQLKDVDRSVALLMLWTRTCFIRRCGRNRRGSRGFSAGSTYLRTPRA
                     PFS"
     gene            3341..3823
                     /locus_tag="VFL84_02885"
     CDS             3341..3823
                     /locus_tag="VFL84_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607597.1"
                     /translation="MVSVFVSRASTSGVSCVFEGAIEPGPRLLAALRRRRAEIPYAAR
                     SDSFRRLVNGWHTFCFFFGMDVTNVLDGRNKWPNRLGKIPGMPLPATSEANLRELRMR
                     YNAAYSAYQSCLIAINEAAMSGLPPSKALLANEGNALRELNDARGNLIAAMGQITWPS
                     "
     gene            4547..>4852
                     /locus_tag="VFL84_02890"
     CDS             4547..>4852
                     /locus_tag="VFL84_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607598.1"
                     /translation="MKIRKIRAFGRRGRVVCAVLFWLAFAGFVIVPIFAVTIRTGMHI
                     AGDVPAAVTRPNNGDGGAYDVLQALLSPLQLLVAVFLILGVVLGVWLAALRELHRLFG
                     "
BASE COUNT          821 a         1575 c         1536 g          920 t
ORIGIN      
        1 gtgacgttgt tctcttcgta cacttcgcag ttcgcacgct ccgccggaat tctgtcggcg
       61 cccggtcctt gggccagcgt cagcacgcgc gtcggcatca cccacggctc gaggaacatc
      121 tccggatctt cgatcgtcat gtcgtagcgg atctcgttgc cgacacgcgt gagccgttcg
      181 acgacatgca tgctcgcgga gtggatgaga ccgccgcgtc ccaaccacgt cgaatcgtcg
      241 aagttgatcg agtcgatgac gagcgtgtcg ccttcccagg ttcccaagct ctgcccgtag
      301 aacgtggtga ggctctcgtc gagcgtgctg cgcttgcggc cgtcgatcgg gatgtcgcgg
      361 tactcgttgt tgccgccgcc gtaatcggcg ttggccggat agaagaagat gacgtctttg
      421 tcggtttgga cgatcctcgt cggcgtgcca tggcgcggca gcccgagcgg cctgcacgtc
      481 atgacggggt cgtacttgtt cgtccacatg tcgagctctt ggatgtggtc ccagagctcg
      541 ggcttgtagt tcggccgacc gaggattccg aatcgcgacg gcgagatcca cgcgtactcg
      601 atccagaagt tgtctccgcc acccgtgcag ccgtactcgg ggttgatgcc gccgcccttc
      661 acctgcgtcg ggccgcaacg gcggttgccg ctacccgaga ccggattgag cgggcgattc
      721 cagttgccgg aaaggtccgg cttcccatcg gcgcgccgcg gcgtcggagc ggcggcgacg
      781 ggcgtgggcg ggttgcgcgg cgttagcgtc cagttcgcag gctcgtgcgt cggcttgatg
      841 gcggctttga cgctcgccgg cacgggcatc acgacgtaga gctggcccgt ctccgtcatc
      901 gtcgtcgtga tgtcgcgcat cacggtgccg ttcttcttct ccgtgatcct gccggtatag
      961 aaagcctcgg cgggatgcag cgggttgttc cagtccttgt agttgccgta cgtgaactcg
     1021 tacgtgtccg cgccgttctt cacgacgacg ctcgaggcca tgaacttcgc atcgagcgtg
     1081 gcggtggccg ttgccgtggg gacgcccgga atcgggaacg tcacgacagg cgtcttgccc
     1141 tgccacgcga ccgaggtctt gtgcaccttc atcacgtccg cgggcacgcg ttgcggtcgc
     1201 gcggccatct gcggcgtgtc cgtatcctga gtgccgaaca tcgcggcctt ccacgcgccc
     1261 tgcgggttgg cccacaggcg aatcatccgc tcctcgacgg cggccggcat cggcgtagct
     1321 ttgccctcgc ccgcgacgag ctcggcgccc atgatgtctt cgttccagct gtacgcacca
     1381 ctcagcactt cgatcgtgtt gcagctctga ccgttcggcc gagtgcccgt gatccgaatc
     1441 cgctcgcccg acgtctggta gctgatgtcg gagcggtaca tcgtcacgtt gcagggctga
     1501 ccgcccactt ggacggtgcc cttgccctga tacatcagcg acatgaggag atcgtattcc
     1561 tcggtgctgc gcagcatgcc catgtgccag gcccagttga acaggacgga cttcaagtcc
     1621 ttggcgtcgg cagcgggcgt gtactccggc ccgccggggc cgccgggccc gccaccgccg
     1681 ccgccgccgc gacgtccggc gccggtaggc ggcgccgtgc tgtattgagg accgaacgcc
     1741 tcgccttctt cttgagcgct ggcgacggac ggcacgccga gcacgagcgt cgcagcgacg
     1801 gcgacgcccg tgcacagcat tcttggtcga gaagccatcg tgatctcccc ccggttcact
     1861 cagttgccgc ccgcgttgcc caagacggtg ccgtccgcgc gcgtcagcga cctcaagccg
     1921 cccaacgggc tgccgttccg tgccggcgtg cccacgacgg tgacctcttc gccgatcttg
     1981 agcgcgttcg ggccaacttt ggtgatccct tgcgaggcga gcgccgtcgc ggaaccggtc
     2041 gtgaacgtcc aatcggtcgt cgtgccgtcg gcgttcttca ccgtcacgac gatcgagccg
     2101 tgcggattca cgaactgcag cttggccagc gcgcccgtga ctttgacctc tttacccggg
     2161 tcgtagacgc ccgcggtgga gtgatgcgcc tgtagcgagc cactggccac caaggacccg
     2221 aacacgacga cagaccccaa agcttgcttg atcatgtgtg agctccccaa aaggtgtgac
     2281 ggcgcgggcg ccatcaatag cgatcgaaaa aaagccgaat taccgccgcc gagcgtgcag
     2341 cacgacccgt ctcaaatcaa gggatggggc aatcgacggc cgttactggc cctcgagctc
     2401 gctcagtaag gcctctagct cgagcttacg agccgccttt cgccggcgtg agcggggcgc
     2461 ccacctgaac aaccacaggc agatgaacgc gaacacgatc aagatcggca acggcgaccc
     2521 atcgctttcg ccgcgcggaa cgtcgttgat tcggtcggca gtgaaggaga tggcccacgc
     2581 gccgatcaac cccgccgccg cgatcaccgc cgcgaggcgg cgctcgcgtt tttcgtcggc
     2641 cccgagcagc tcgatacggc ggcgcagatg atcccgaagc gagtcgccaa agccctgcgc
     2701 gcgccagttg agcccggcgc atcaccaaca gtgcgcccgc cagcacgacc gacgcgacca
     2761 cgccgacgaa gccgacgacg taatcccaaa cgatcagcac gtcgtcgtcg ttgggatcga
     2821 tcaggatggc gagaaacaac gcggcggtga tcaagagaac ggcgctcgag gcgtacgcaa
     2881 ataagttgac cctgcgctga aacctctcga gcctcgcctg ttcctgccgc agcgcctgat
     2941 gaagcaggtt ctcgtccaga gcatcagcag cgcgaccgag cggtcgacgt ccttgagctg
     3001 cgcgatccgc ctatacagcc agtcgacgcg cggatcaggc tctgcgccgg acacggtgag
     3061 aaggccttcg actatctcga gcggctgctt gccgcgctga tgccggccct gtttgcgcgt
     3121 ccacgagatg gccgcgttca gggcgatgcg gtacatccag gtcggcacgg aggagtcgcc
     3181 gcggtacgaa tccaccgagc gccagacctg gattgcgatt tcctgaaaga ggtcctggcg
     3241 atccgcgtgc ttgaacgcgt aggcgcggac gaccttgaaa aggattcccc tatgcgctgc
     3301 gagccagtcg ccgaagatgc ggtcccgttc gctgtccagc atggtctcgg ttttcgtttc
     3361 ccgcgcgagc acatccggcg tgagctgcgt tttcgaaggc gccattgaac cgggtccccg
     3421 acttcttgcc gctcttagac gcaggcgggc ggagattcct tacgcggctc ggagcgacag
     3481 ttttcgtcga ctcgtcaacg gctggcacac cttttgcttt tttttcggca tggacgttac
     3541 gaacgttctc gatggccgca acaagtggcc gaaccggctt ggcaaaatac cgggaatgcc
     3601 gctacccgct acatctgaag cgaacttgcg cgagctgcgc atgcggtaca acgccgcgta
     3661 ttccgcatat cagagctgcc tcatcgctat taacgaagcc gctatgagtg ggctgcctcc
     3721 ctccaaagca ctgctggcga acgagggcaa cgccctccgg gagttgaacg atgcgcgcgg
     3781 caatctgatc gcggcgatgg ggcaaatcac ctggccaagc tgagggtgca acgggctgtt
     3841 atgcgccgcc gattgccgta cgagtccaag gagctgctac gagagctgac gcggtggatc
     3901 gaatggcgct atgccaatac gaaggaccac gtcgttttaa cttccgcccc gacctcgctg
     3961 ccgtcgaatc tagagaactg gatgcccgac gcggtatggc ggctggcttg gttcaagagc
     4021 ggtgccgcgg ttctgttcgg cctcgctccg ggcgaccggc cgggacgata tttcgtggtg
     4081 cgtcacgatg ccgtcaccgc acgcagagaa ggtaccttcg aaaaggattc cgacagcacc
     4141 tggcgaaaca ccgacggcga cagcttggaa ccagcgagca gcggcgtgcg acgcacgacg
     4201 atcgtcagac ccggccacgg tcgttaccgc gctcggcgcc gtagctcgag aacacggtcg
     4261 agcagcggcc ccgcgcgtcg gccaccgaag cgctcccgct gagacggggc gggaaggcgc
     4321 cggccggaaa gctggggccc ggtcattttc gctccaccag tcaatctttt acggcagccg
     4381 ccgcctatgg cgggacatcg ttaatcgact gctggatatc gataaacgat aataaattgt
     4441 tgcaaatcgt taagctcacg cttaggatct ccgtcgccaa gttggccaag gggtcgctcc
     4501 gatgaacgct tcagccgcag cgctgcctcg cgaagacgag gttcaattga agattcgcaa
     4561 gattcgcgca ttcggccgca ggggccgcgt cgtctgcgca gtcctctttt ggttggcctt
     4621 cgccggattc gtcatcgtgc caatcttcgc cgtcaccatt cggaccggga tgcacatagc
     4681 gggcgacgta ccggccgctg tcaccaggcc gaacaatggc gacgggggtg cttacgatgt
     4741 cttgcaggcc ctgctctcgc cgctgcagtt gctcgtggcg gtgttcctga ttctcggtgt
     4801 cgtgctcggc gtctggctcg cggctttgcg cgagctccat cgcctgttcg gc
//
LOCUS       DASSNN010000164         5263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_32777, whole genome shotgun sequence.
ACCESSION   DASSNN010000164 DASSNN010000000
VERSION     DASSNN010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32777"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1552)
                     /gene="metG"
                     /locus_tag="VFL84_02895"
     CDS             complement(<1..1552)
                     /gene="metG"
                     /locus_tag="VFL84_02895"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET7607599.1"
                     /translation="MSVKRTILVTSALPYANDSLHLGHILEYIQTDVWVRFQRLCGHR
                     CLYVCASDAHGTPTMLRAEQEGIKPEDLVARVTAEHKRDFATYRISVDNYLTTHSPEN
                     EELTTELYRRLAAGGFIARKTIKQAYDDKRQMFLPDRYVKGTCPNCGAAEQYGDSCEN
                     CGATYSPLELKDAVSTVSGTKPSVRESEHLFLKLSAFEDELKRWVPEHVDEALARKLD
                     EWFKAGLKDWDISRDAPYFGFRIPGERDKFFYVWFDAPIGYMASFLNLCRRENIDFEE
                     FWGAASKHELYHFIGKDIPYFHALFWPAMLSGAGYRKPSGLFVHGHLTVDGQKISKRR
                     GNAITAGDFAKKIDPDYLRYYYTSKLGSSIDDLDLNLADFVAKVNADLVGKLVNIASR
                     CAGFIEKHGGGRLADKLPEPALYAEFAGAADDLAADFEQREYSRAVRKIMALADRANQ
                     YIDDKKPWIMAKDPSAAPDVVAVCTMGLNLFRALIVYLKPVVPALAARAEALLAGGEL
                     DWSSAAAPL"
     gene            complement(1624..2688)
                     /locus_tag="VFL84_02900"
     CDS             complement(1624..2688)
                     /locus_tag="VFL84_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET7607600.1"
                     /translation="MAASLINDFLKSMYMRKGSDLHVIAGDPPRMRVHGDLITLDNQR
                     LDPEELKAALLGIMSEQSVEQFNKHDSADFAHSIPDVARFRVNVFRHLNGIGAVFRGI
                     PSKALSLEELKMPKSIHELSTATRGLILVTGKTGSGKSTTLAAVIDAINAREKGHILT
                     IEDPIEFVHQRKLCLISQREVGVHSPSFANALHSALREDPDAILVGELRDLETMSIAM
                     TAAEMGVLVMGTLHTNGAAATIDRAVNIFPADKQDHVRNMLSTSLRGIVSQQLCKKKD
                     GTGRCVALEILINTSAAANLIRQGKLEQLENVMQSSGREGMCTMDSSLKKLYDDGMIT
                     GQEAYLNAYDKAKFESIKDD"
     gene            complement(2702..3859)
                     /locus_tag="VFL84_02905"
     CDS             complement(2702..3859)
                     /locus_tag="VFL84_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET7607601.1"
                     /translation="MARIDAFLKLGLAQGCSDIHLAVGVPPMLRLHGDLMPIKFRDLG
                     DTELEGYVDEILSESQRQRFRQKQDIDFSYVSSDGGRFRVNVFHKATGVGATFRNIPS
                     VVPSLDSLNLPPILHKFCDLHQGMVLVTGSTGTGKSTTLAAMIDHINTTSNSNIISLE
                     DPIEFVHSSKRSQIIQRELGTHIPSFAEGVRASLREDPDVILVGELRDGETISMAMTA
                     AETGHLVLGTLHTTSAVKSIDRIIDALPGDQREQTKSFLANSLHAVVTQNLIKTPDGR
                     GRRAIVEIMVMNRAIAKLIQTEQTHQIPAQLQTGKDQGMQMMDQALLAAIQAKLVDPD
                     DAYRYAVDRKKFERFVTDTTVLPSGTSTTATPAPAAAQAAPKSASTGTDAD"
     gene            complement(3864..>5263)
                     /locus_tag="VFL84_02910"
     CDS             complement(3864..>5263)
                     /locus_tag="VFL84_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7607602.1"
                     /translation="SERAADALAEIGDAKALPALLEMMAKNNRSLPVALTAVGKLGNA
                     SILPKIIPYLQRPEKEVRVAAIGAVAQLAGEQQAEAVRGPLQQSAQGADETVQRAVSK
                     ALQKLEGRLSPSGRFTGTATFAGPGPTATATPPPMPTPPTARAQTRSAPPPSPAATST
                     ARTLLVDGPEDAVAEPPAPEVPSFDLNALQNGDMIEGRYKFIQKIGKGAFGTVVLVED
                     TVVEERLILKFLNANVSSDEEMLKRFVHELRYSRRITHKNVIRIYDFLYVGGSYAISM
                     EYFPSHTLGTEIANEKPMSPKKALNYGTDICVGMSVAHQQGIIHRDLKPANILINDSG
                     LLKIVDFGVAAAAKSGDTQLTKTGYVIGSPKYMAPEQILGKKVDERADIYSLGVIMYE
                     MLAGNPPYSRGDHMSVMYQHVQGKAQPLNEANPKVPREIADVVTKTMQVDKLKRYASM
                     EELRIALEAAAGAIR"
BASE COUNT          809 a         1641 c         1753 g         1060 t
ORIGIN      
        1 gcagcggagc ggccgccgag ctccagtcga gctcgccgcc ggcgagcaag gcctcggcgc
       61 gcgccgcgag cgcgggcacg acgggcttca aatagacgat cagggcgcgg aacaggttca
      121 gccccatcgt gcacacggcg acgacgtcgg gcgctgcgct cggatccttc gccatgatcc
      181 acggcttctt gtcgtcgatg tactgattcg cgcggtcggc gagcgccatg atcttgcgga
      241 cggcgcgcga gtactcgcgc tgctcgaagt cggcggcgag gtcgtcggct gcacccgcga
      301 actcggcgta gagcgcgggc tcgggcagct tgtcggccaa gcgcccgccg ccgtgcttct
      361 cgatgaagcc cgcgcagcgg cttgcgatgt tcacgagctt gccgacgaga tccgcgttga
      421 ccttggcgac gaagtcggcg aggttcagat cgagatcgtc gatcgaggac ccaagcttcg
      481 acgtgtagta gtagcgcagg taatccggat cgatcttttt cgcgaagtct ccggccgtga
      541 tcgcgttgcc gcggcgcttc gagatctttt gcccgtctac cgtcaggtgc ccgtgcacga
      601 acaagccgct cggcttgcga taacccgcgc cggagagcat cgccggccag aacagcgcgt
      661 ggaaatacgg aatgtccttg ccgatgaagt gatagagctc gtgcttgctc gccgcgcccc
      721 agaactcctc gaagtcgatg ttctcgcggc gacacaggtt caaaaagctc gccatgtaac
      781 cgatcggcgc gtcgaaccag acgtagaaga acttgtcgcg ctcgcccgga atccggaagc
      841 cgaagtacgg cgcgtcgcgc gagatgtccc agtccttgag ccccgctttg aaccactcgt
      901 cgagcttgcg cgcgagcgct tcgtcgacgt gctcgggcac ccagcgcttc agctcgtcct
      961 cgaaggcgct caacttcaag aacaggtgct cggattcgcg cacggacggc ttcgtgcccg
     1021 acacggtgga cacggcgtcc ttgagctcga gcggcgaata cgtggcgccg cagttttcgc
     1081 acgagtctcc gtactgttcc gcggcgccgc agttcgggca cgtgcccttg acgtagcgat
     1141 ccggcaggaa catctgccgc ttgtcgtcgt aggcctgctt gatcgtcttg cgcgcgatga
     1201 agccgccggc cgcgagccgc cgatagagct ccgtcgtgag ctcttcgttc tcgggcgaat
     1261 gcgtcgtcaa gtaattgtcg acgctgatcc ggtacgtcgc gaagtcgcgc ttgtgctcgg
     1321 ccgttacgcg cgcgacgagg tcctcgggct tgatgccctc ctgctcggcg cgcagcatcg
     1381 tcggcgtgcc gtgcgcgtcg ctcgcgcaga cgtacaagca gcggtgcccg cagaggcgct
     1441 gaaagcgcac ccagacgtcg gtctggatgt actcgagaat gtgccccaag tgaagggagt
     1501 cattcgcgta cggcagggcg ctcgtaacga ggatagtgcg cttgacgctc aagactggga
     1561 ggctccgcaa cgtggatcgg cagcgtccgt tgctgccgcg cggacattat gccatcggca
     1621 aggtcaatcg tctttgatcg actcgaactt cgccttgtcg taggcgttta gatacgcctc
     1681 ttggcccgtg atcattccgt cgtcgtagag cttcttgagc gagctgtcca tcgtgcacat
     1741 gccttcgcgc ccgctcgatt gcatgacgtt ctcgagctgc tcgagcttgc cctggcgaat
     1801 gaggttcgca gcggccgagg tattgatcag gatctcgagc gcgacgcagc ggcccgtgcc
     1861 gtcttttttc ttgcagagct gctgagacac gatgcccctg agcgacgtcg acagcatgtt
     1921 gcggacgtgg tcctgcttgt cggccggaaa gatattcact gcccggtcga tcgtcgcggc
     1981 cgcgccattg gtatgcagcg tgcccatgac gagcacgccc atctcggcgg ccgtcattgc
     2041 gatgctcatg gtctcgaggt cgcgaagctc gccgacgaga atcgcgtcgg gatcctcgcg
     2101 caacgcggag tgcagggcgt ttgcgaagct cgggctgtgc acgccgacct cgcgttggct
     2161 gatcaggcag agcttgcgct ggtgcacgaa ctcgatcggg tcctcgatcg tcaaaatgtg
     2221 gcccttctcg cgcgcgttga tcgcgtcgat gacggccgca agcgtcgtcg atttgccgga
     2281 gccggtcttg cccgtcacga gaataaggcc gcgcgttgca gtcgagagct cgtgtatcga
     2341 cttcggcatc ttgagctctt cgagcgacag ggctttcgaa gggatgcctc tgaataccgc
     2401 gccgatgccg ttcaagtggc ggaacacgtt gacgcggaag cgggcgacgt ccggaatcga
     2461 gtgcgcgaag tccgccgagt cgtgcttgtt gaactgctcg accgattgct cggacatgat
     2521 cccgagcagc gcggctttga gctcctcggg gtcgagacgc tgattgtcga gcgtgatcag
     2581 atcgccgtga acgcgcatgc gcggcgggtc gcctgcgatg acgtgcaggt ccgagccctt
     2641 gcgcatgtac atgctcttca gaaagtcgtt gatcaacgac gctgccatcg ccggctcctg
     2701 ctcagtccgc gtccgtgccc gtgctcgcac tcttgggcgc cgcttgtgcg gccgccggtg
     2761 caggggtcgc cgtcgtgctc gtgccgctcg gcagcacggt cgtatcggtc acgaagcgct
     2821 cgaacttctt gcggtcgacg gcgtagcggt aggcgtcgtc ggggtcgacg agcttggcct
     2881 ggatggcggc gagcagcgcc tggtccatca tctgcatgcc ttggtctttg cccgtctgca
     2941 gctgggcggg aatctggtgc gtctgctcgg tttggatgag cttcgcgatc gcgcgattca
     3001 tgaccatgat ctcgacgatc gcgcgccggc cgcggccgtc cggcgtcttg atcaggttct
     3061 gcgtcacgac cgcgtgcaag ctgttcgcga ggaagctctt cgtctgctcg cgttgatcgc
     3121 ccggcagcgc gtcgatgatg cggtcgatcg acttgacggc gctcgtcgtg tgcagcgtgc
     3181 cgaggaccag gtggcccgtc tcggcagccg tcatcgccat gctgatcgtc tcgccgtcgc
     3241 gcagctcgcc gacgaggatc acgtcggggt cctcgcgcag gctcgcgcga acgccctcgg
     3301 cgaagctcgg gatgtgcgtg ccgagctcgc gctggatgat ttggctgcgc ttgctcgagt
     3361 gcacgaactc gatcgggtcc tctaggctga tgatgttcga attgctcgtc gtgttgatgt
     3421 gatcgatcat cgcggcgagc gtcgtcgact tgccggttcc cgtcgatccg gtgacgagca
     3481 ccattccctg gtgcaagtcg cagaacttgt gcaggatcgg cggcaggttg aggctgtcca
     3541 agctcgggac gacgctcggg atgttccgga acgtcgcgcc gacgcccgtc gccttgtgga
     3601 acacgttgac gcggaagcgt ccgccgtcgc tgctcacgta cgagaagtcg atgtcctgct
     3661 tttggcggaa tcgctggcgc tgcgattcgc tcaagatctc gtcgacgtag ccttcgagct
     3721 ccgtgtcgcc gagatcgcgg aacttgatcg gcatcaagtc gccgtgcagg cgcagcatcg
     3781 gcgggacgcc gacggcaaga tggatgtccg agcacccttg cgcgaggccc agcttcaaga
     3841 acgcgtcgat acgtgccatg cggttacctg atcgcccccg ccgcggcctc gagggcgatg
     3901 cgcagctctt ccatcgacgc gtagcgcttg agcttgtcga cttgcatcgt cttcgtgacg
     3961 acgtcggcga tctcgcgcgg caccttcgga ttggcctcgt tgagcggctg cgccttgcct
     4021 tgcacgtgct gatacatgac cgacatgtga tcgccgcggc tgtaaggcgg gttgcccgcc
     4081 agcatctcgt acatgatgac gcctaagctg tagatgtcgg cgcgctcgtc gactttcttg
     4141 ccgagaatct gctcgggcgc catgtatttc ggtgagccga tcacgtagcc cgtcttcgtg
     4201 agctgcgtgt cgccgctctt cgcggcggcg gcgacgccga agtccacgat cttgagcagc
     4261 ccggagtcgt tgatgaggat gttcgccggc ttcaagtcgc ggtggatgat gccctgttga
     4321 tgcgcgacgc tcatgccgac gcagatgtcc gtcccatagt tcagcgcttt cttcggcgac
     4381 atgggcttct cgttggcgat ttccgtgccg agcgtgtgcg acgggaaata ttccatcgag
     4441 atcgcgtagc tgccgccgac gtacaagaaa tcgtagatgc ggatgacgtt cttgtgcgtg
     4501 atgcggcgcg agtaacgcag ctcgtggacg aagcgcttca acatctcctc gtcggacgag
     4561 acgttggcgt tcaagaactt cagaatgagg cgctcctcga cgacggtgtc ctcgacgagg
     4621 acgacggtgc cgaaggcgcc tttgccgatc ttttggatga acttgtagcg gccttcgatc
     4681 atgtcgccgt tctgcagcgc attgagatcg aacgacggca cttcgggggc cggcggctcg
     4741 gccacggcgt cttcggggcc gtcgacgagc agcgtgcgcg ccgtcgacgt cgcggccggc
     4801 gacggtggcg gcgccgagcg cgtttgggct cgagccgtcg gcggcgtggg catcggtggc
     4861 ggcgtggccg tcgcggttgg gccgggcccg gcgaacgtcg ccgtgcccgt gaagcggccg
     4921 ctcgggctca accggccctc gagtttctgc agcgccttcg agaccgcgcg ctgcacggtt
     4981 tcgtcggccc cttgcgcgct ctgctgcaac ggaccgcgca cggcctcggc ttgctgctcg
     5041 ccggccagct gggcgacggc gccgatcgcg gcgacgcgaa cctctttctc gggccgctgc
     5101 aagtacggaa tgatcttcgg caggatggaa gcattgccga gcttgccgac ggccgtgagg
     5161 gccaccggca gcgagcgatt gttcttcgcc atcatctcga gcaacgccgg cagtgccttg
     5221 gcgtcgccga tctcggccaa cgcatcggcc gcgcgttcgc tga
//
LOCUS       DASSNN010000165         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_898726, whole genome shotgun sequence.
ACCESSION   DASSNN010000165 DASSNN010000000
VERSION     DASSNN010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898726"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFL84_02915"
     CDS             <1..695
                     /locus_tag="VFL84_02915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET7607603.1"
                     /translation="SVLPSPFDYERNARLYLPQGLPDPADERYVETLLALTWPLVEAA
                     QGGAFLLFTSYRALQRAERWLEGRAAPGRVLIQGRGSRSELLNQFRADGNAILLGTGS
                     FWQGVDVRGRALRLVVIDKLPFAVPSDPLVQARAEAIRRAGGDAFNELQLPQAVLALK
                     QGVGRLIRDFDDRGLIVLGDPRLRTRPYGATFLASLPAMPVIDELAEALQFAATLADD
                     AVREPAAAVAAS"
     gene            692..1369
                     /gene="tsaB"
                     /locus_tag="VFL84_02920"
     CDS             692..1369
                     /gene="tsaB"
                     /locus_tag="VFL84_02920"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET7607604.1"
                     /translation="MNMLAFETSSAVGSVALETAAGVVVRDLGIPREQTERLLAVTDE
                     LLNDARIELGSLDGIAFGRGPGSFTGLRVSVAVAQGLGAASGVPLLPVSSLLCLAERA
                     WQEAGCERALVCVDAHMGEVYWAHAARRGDEVEILGDERLSAPADVAPFEEAPWCAVG
                     NGFAAHAATLAPIARAAAAVLPELVPSAAALLPRAKRDLIAGRATPPSAALPVYLREQ
                     TAWRRSS"
     gene            1540..2232
                     /gene="phoB"
                     /locus_tag="VFL84_02925"
     CDS             1540..2232
                     /gene="phoB"
                     /locus_tag="VFL84_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HET7607605.1"
                     /translation="MTQRRILVVEDERAIREMVCFNLGRAGYDVAPAADAREARAAIA
                     DRRPDLVLMDWMLPDVSGLELTRQLKRDPETKEIPVIMLTARGEENDRVAGLDGGADD
                     YVVKPFAPRELLARIRAVLRRLEAADAEVVTAGTLKLDSASHRVSAGGAEVALGPTEY
                     RLLEFFMRHSDRVYSRAQLLDRVWGGNVYVEERTVDVHIRRLRMALTPTGHDSFIRTV
                     HGAGYRFSVATP"
BASE COUNT          331 a          792 c          801 g          365 t
ORIGIN      
        1 agagcgtgct gccgagcccg ttcgactacg agcgcaacgc gcggctctac ttgccgcaag
       61 gcttgcccga tcccgcggac gagcgctacg tcgagacgct gctcgcgctc acctggccgc
      121 tcgtcgaagc cgcgcagggc ggggcgttcc tcttgttcac gagctaccga gcgctgcagc
      181 gcgccgagcg ctggctcgaa ggccgcgctg cgccgggacg tgtgctgatc cagggccgcg
      241 gatcgcgcag cgagctgctg aaccagttcc gcgccgacgg caacgcgatc ctgctcggca
      301 cgggcagctt ctggcagggc gtcgacgtgc gcggccgcgc gctgcggctc gtcgtcatcg
      361 acaagctgcc gttcgcggtg ccgagcgatc cgctcgtgca ggcgcgtgcg gaagcgatcc
      421 gccgcgccgg cggcgacgcg ttcaacgagc tgcagctgcc gcaagccgtg ctcgcgctga
      481 agcaaggcgt cggccggctc atccgcgact tcgacgaccg cggcctcatc gtgctcggcg
      541 atccgcgcct gcgcacgcgg ccgtacggcg cgacgttcct cgcgagcctg cccgcgatgc
      601 ccgtcatcga cgagctcgcc gaggcgcttc agttcgccgc gactctcgcc gacgacgccg
      661 tacgagagcc tgccgcggcc gtggccgcat catgaatatg ctcgcgttcg agacgtcgag
      721 cgccgtgggc tccgtggcgc tcgagacggc ggccggggtc gtcgttcgcg atctcggcat
      781 cccgcgcgag caaaccgagc ggttgctcgc cgtcaccgac gagctgctga acgatgcgcg
      841 catcgagctc ggcagcctgg acgggatcgc gttcggccgc ggccccgggt cgttcacggg
      901 gctgcgcgtc agcgtcgccg tggcacaagg tttgggcgcg gcgagcggcg tgccgctgct
      961 gccggtgtcg agcttgcttt gcctcgccga gcgcgcgtgg caggaagcgg gctgcgagcg
     1021 cgcgctcgtc tgcgtcgacg cccacatggg cgaggtctac tgggcgcacg ccgcgcggcg
     1081 cggcgatgaa gtcgagatcc ttggcgacga gcgcttgagc gcgccggccg atgtcgcgcc
     1141 attcgaggag gcgccgtggt gcgccgtcgg caacgggttc gcggcgcatg ccgcgacgct
     1201 ggcccccatc gcgcgcgcgg ccgcggccgt cttgccggag ctcgttccgt ccgccgcggc
     1261 cctgttgccg cgcgcgaagc gcgatctcat cgccgggcgc gccacgccgc cgtcggccgc
     1321 gctgcccgtc tacttgcgcg agcagaccgc gtggcggcgc agctcttaac gcgactgtaa
     1381 tattgctgcg ctccaatgcc cgccatgaaa atggggacag atctattttc ggaccgcaaa
     1441 aatgaaattg gggacagatc tattttctga gggcaacaga aaaggggaca gatttactct
     1501 cctagaaaat agatctgtcc ccttttgcag aaggtggcga tgacgcagag gagaatcctc
     1561 gtagtcgagg acgagcgcgc gatccgcgag atggtctgct tcaatctcgg gcgcgccggg
     1621 tacgacgttg ctccggccgc cgacgcgcgc gaggcacgcg cggcgatcgc ggatcgacgg
     1681 cccgatctcg ttttgatgga ttggatgctg cccgacgtca gcggtctcga gctgacgcgc
     1741 cagctcaagc gcgatcccga gacgaaggag attcccgtca tcatgttgac ggcgcgcggc
     1801 gaggaaaacg accgtgtcgc cggtctcgac ggcggcgccg acgattacgt cgtgaagccg
     1861 ttcgcgccgc gcgagctgct cgcgcggatc cgcgccgtgt tgcgccgcct cgaagccgcc
     1921 gatgccgagg tcgtcacggc cggcacgctg aagctcgact ccgcgagcca ccgggtcagc
     1981 gccggaggcg ccgaggtcgc gctcggtccc accgaatacc ggctgctcga attcttcatg
     2041 cgccactcgg accgcgtcta cagccgtgcg cagctgctcg accgcgtctg gggcggcaat
     2101 gtctacgtcg aggagcgaac cgtcgacgtc cacattcgcc ggttgcgcat ggcattgacg
     2161 ccgacgggac acgactcgtt catcaggacc gtgcacggcg caggttaccg gttctccgtc
     2221 gccacgccgt gaacctgccg agtcgctgga cctcgcagat cgcggcgctc gctgcgctgc
     2281 tcgccgcgg
//
LOCUS       DASSNN010000166         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1419727, whole genome shotgun sequence.
ACCESSION   DASSNN010000166 DASSNN010000000
VERSION     DASSNN010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419727"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFL84_02930"
     CDS             complement(<1..305)
                     /locus_tag="VFL84_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7607606.1"
                     /translation="MADSIAAALTRIAAIVGPRGIVPPEDAAPLLRDHRELYRGAAAL
                     VVRPGTVDECASVVRVCHDARIGVVPQGGNTSYCGGATPFDGARRQILLSLARLNRVR
                     "
     gene            complement(375..>2758)
                     /locus_tag="VFL84_02935"
     CDS             complement(375..>2758)
                     /locus_tag="VFL84_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7607607.1"
                     /translation="RWVFDSARPRIDDDGTFRGYVGVVVDITELKQKELDLRHSREQL
                     ALLSDTVPALISYIGVDRRYRTCNALYLKWFGLTAEQVVGQRIEDVLGPKAWGVVAPY
                     FERALSGEPCEYEAQIEYRLGGKRWVHARYTPDREDNGDVAGVVCLVTDISARREAGH
                     ARSRLAAIVDSSDDAIVSKTLDGTITSWNLGAERLFGYTAAEAIGQPVTMLIPKDRQY
                     EEQQILERIRHGQTVPPYETIRRRKDGTVFDLSLSVSPIIDEEGHIVGASKIARDITA
                     RKRAEQDIRRSREALNGLIDRAPFGLYIVDSDLKIAQMNARSQSGAFYNVRPVIGRKL
                     DEVMRFIWPEPVATEIVSAFRETLESGETYRSRNFMSQRADTDNVESYEWELHRITLP
                     DGKLGVVSYYFDTTRLRKAEQGLREADRRKDEFLATLAHELRNPLAPIRNGLQIVRMA
                     GKDPVASAQVQEMLERQVSHLVRLVDDLMEVARVTRGRIELRREPVDLGAMLRSAVET
                     SKPLIDEAGHELVVDLPEEPVTLIADPVRLAQIVSNLLNNAAKYTENGGRISLSARRE
                     GNHAVISVRDTGVGIPIEVLPRVFDLFAQADRTYHRAKGGLGIGLTLVRTLVELHGGT
                     VLAKSDGVGRGSEFIVRLPLGIELANRRDSVRDDSDTVFAAHRFLVVDDSRDAADSLA
                     LLLESLGADVHTATDGPAALDELDTYRPSVMLLDIGMPGMDGLEVARRARQRSDSRDL
                     TVIALSGWGQDEDRRRSREAGIDYHMVKPVDLDELGRLLTALAPSRSHRMRLI"
BASE COUNT          431 a          930 c          932 g          465 t
ORIGIN      
        1 cgcacgcggt tcaagcgcgc gagcgacagc agaatctgcc ggcgcgcgcc gtcgaacggc
       61 gtggcgccgc cgcaatagct cgtgttgccg ccctgcggaa cgacgccgat gcgcgcgtcg
      121 tgacagacac gcacgacgct cgcgcattca tcgacggtgc cgggacgcac gacgagcgcc
      181 gccgcgccgc gatagagctc gcggtggtcg cgcaagagcg gcgccgcatc ctcgggcggc
      241 acgatgccgc gcgggcccac gatggcggcg atgcgcgtca gtgcggcggc aatcgaatcg
      301 gccatctcgg gttccaaaac cggtgcggcg ccgatattac accggcgcgc tgcgggcgtg
      361 cgggctacgc ggcttcagat cagccgcatg cggtgtgagc gcgacggcgc gagcgcggtc
      421 aggagccggc cgagctcgtc caagtcgacg ggtttgacca tgtgatagtc gatgccggct
      481 tcgcgcgaac ggcgccgatc ttcgtcctgc ccccatcccg acaacgcgat gacggtgagg
      541 tcgcggctgt cggaccgctg ccgcgcgcgc cgcgcaacct cgaggccgtc catgccgggc
      601 atgccgatgt cgagcagcat gacggacggc cgataagtgt cgagctcgtc gagcgccgcc
      661 ggtccgtccg tcgcggtgtg cacgtcggct ccgaggctct cgagcagcag agcgagactg
      721 tcggccgcgt cgcggctgtc gtcgacgacc aaaaaacgat gcgccgcgaa cacggtgtcg
      781 ctgtcgtcgc gtacgctatc gcgccgattc gcgagctcga tgccgagcgg caagcggacg
      841 atgaactcgc tgccgcggcc gacgccgtcg ctcttcgcca aaacggtgcc gccatgcagc
      901 tctacgagcg tgcgcacgag cgtgagccct atgccgagcc cccctttggc gcggtggtag
      961 gtgcgatcgg cctgcgcgaa caagtcgaag acgcgcggca agacctcgat cgggatgccg
     1021 acgcccgtgt cgcgcaccga gatcaccgcg tgattgccct cgcgccgtgc cgacagcgag
     1081 atgcggccgc cgttttccgt gtacttcgcg gcgttgttca gcagattcga gacgatctgc
     1141 gcgagccgca ccggatccgc gatgagcgtc acgggctctt cgggcagatc gacgacgagc
     1201 tcgtgacccg cctcatcgat gagcggcttg ctcgtctcga ccgcgctgcg cagcatcgcg
     1261 cccaaatcga caggctctct gcgcagctcg atgcggccgc gcgtcacgcg cgcgacctcc
     1321 atgagatcgt cgaccagccg caccaagtgg ctgacctggc gctcgagcat ttcctgcact
     1381 tgcgccgaag cgacgggatc tttgcccgcc atgcgcacga tctgcaatcc gtttcggatc
     1441 ggggcgagcg gattgcgcag ctcgtgcgcc agcgtcgcga ggaactcgtc cttgcgccgg
     1501 tccgcttcgc gcagcccctg ctccgccttg cgcaaacgag tcgtgtcgaa gtagtacgag
     1561 acgacaccga gcttgccgtc cggcagcgtg atgcggtgga gctcccactc gtacgactcg
     1621 acgttgtcgg tatcggcgcg ttggctcatg aaattgcgcg aacggtacgt ctcgccgctc
     1681 tcgagcgtct cgcggaatgc ggagacgatc tccgtcgcga cgggctccgg ccatatgaac
     1741 cgcatgacct cgtcgagctt gcggccgatg acgggccgaa cgttgtagaa cgcgccggac
     1801 tgcgagcgtg cgttcatctg cgcgatcttg aggtcgctgt cgacgatgta taggccgaac
     1861 ggcgcgcggt cgatcaggcc gttcagcgcc tcgcggctgc ggcgtatgtc ctgctcggcg
     1921 cgcttgcgcg ccgtgatgtc gcgcgcgatc ttcgaagcgc cgacgatgtg tccctcttcg
     1981 tcgatgatcg gcgagaccga cagcgataga tcgaacaccg tgccgtcctt gcggcggcgt
     2041 atggtctcgt agggcgggac cgtctgcccg tgccggatgc gctcgaggat ctgctgctcc
     2101 tcgtattgcc ggtctttcgg tatcagcatc gtgaccggct gcccgatggc ttccgctgcg
     2161 gtgtagccga acagacgctc ggcgcccaag ttccagctcg tgatcgtgcc gtcgagcgtt
     2221 ttgctcacta tcgcgtcgtc ggaggaatcg acgatggccg caagccgcga gcgcgcgtgc
     2281 cctgcctcgc ggcgcgccga gatgtccgtg acgaggcaga cgacgccggc gacgtcgcca
     2341 ttgtcttcgc gatccggcgt atagcgcgcg tgcacccagc gcttgccgcc gaggcgatac
     2401 tcgatctggg cctcgtactc gcacggctcg ccggaaaggg cgcgctcgaa gtacggggcg
     2461 acgacgcccc aggctttggg acccagcacg tcctcgatcc gctgtccgac gacctgctct
     2521 gccgtcaacc cgaaccactt cagatacagc gcattgcagg tgcgatagcg gcgatcgacg
     2581 ccgatgtacg agatcagcgc cggcaccgta tcgctcaaca gtgcgagctg ctcgcggctg
     2641 tggcgcaggt cgagctcctt ttgcttgagc tccgtgatgt cgacgacgac gccgacgtaa
     2701 ccgcggaacg tgccgtcgtc gtcgatgcgc ggccgggcgg agtcgaaaac ccagcggt
//
LOCUS       DASSNN010000167         5035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1656076, whole genome shotgun sequence.
ACCESSION   DASSNN010000167 DASSNN010000000
VERSION     DASSNN010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656076"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..129
                     /locus_tag="VFL84_02940"
                     /pseudo
     CDS             1..129
                     /locus_tag="VFL84_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF615 domain-containing protein"
     gene            216..698
                     /locus_tag="VFL84_02945"
     CDS             216..698
                     /locus_tag="VFL84_02945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7607608.1"
                     /translation="MAQLQWTAPPEMKIDVAKDYAVSIETNRGTIELALYPKHAPQTV
                     NNFVFLAGQGFYDGVKFHRVIADFMIQTGDPTGTGRGGPGYKFGDETKSNPLKHERGV
                     ISMANAGPNTNGSQFFITHVPTPHLNGKHTVFGKVTKGLDVVDAIRQGDTMVKVKVAD
                     "
     gene            complement(753..1712)
                     /locus_tag="VFL84_02950"
     CDS             complement(753..1712)
                     /locus_tag="VFL84_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7607609.1"
                     /translation="MGLIPSGNTVELDLRQLRAFEVLLRERNLTRAAAVLGVAQPALS
                     KTLAKLRRYFADPLFVRAGHRMEPTAKAVELADAVHALLDDATMLRARHRPFDPKTST
                     RTFSFSVVDSGLVHLLPALLKHLETHAPGVRLRVTPIEFEELEAALEAGHLDFAMGSF
                     RSRSKRIRRQALWSVGYVSVVRRDHPRITAKPSLAAFAAERHVLVSTARTGHEHQLVE
                     RALERAIPEEHIVCRVPTFMAAAYAASRTDVVATVPAGTASELSDALRLRVFATPIRL
                     PRIEVAQHWHERFHREPGSRWIRGVFTDLFSKAGDGSVQQLLA"
     gene            1790..2194
                     /locus_tag="VFL84_02955"
     CDS             1790..2194
                     /locus_tag="VFL84_02955"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7607610.1"
                     /translation="MTRTIKLTAAALLFGVGLPAAAHHSFAIFEHSKTYTLKGTVQSF
                     QWTNPHGYIDLEVADGPPGIDHYTLELTSINMLRRAGWKSSDVSVGDKVTAIVAPLMN
                     GQHGGLLLELKMEDGRTLVPPVPAINTFKRTE"
     gene            2199..2957
                     /locus_tag="VFL84_02960"
     CDS             2199..2957
                     /locus_tag="VFL84_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607611.1"
                     /translation="MKAPTRRIALAAVAAATLAVSAVAAAQSGARPDLTGSWERYRGP
                     AGDATNPPPESQPPLKPDLLAEWQKRRQAEREADARGEPLATNYTHCIPDGVPSMMSG
                     PFPFEIVQSASQINIAQEAYSQVRRIYLGKPQVTLDDIELGFYGRSVGEWRGDELLVD
                     TIGIKDYVRFRDVPHTPQMRIKERFKLVRPDILWDEITIEDPAVLEKPWTVTFAYRRM
                     PDYEIIEYVCEDNREYADEEGATRLRLLERQESR"
     gene            complement(3141..3437)
                     /locus_tag="VFL84_02965"
     CDS             complement(3141..3437)
                     /locus_tag="VFL84_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607612.1"
                     /translation="MISADDGVAAARQLEAHGIGASFVGVVLDQPAAQTAGLDADERV
                     GLRIEVRRTAEHLDGDRVTLEPVALTGQRLLGDVAQERRRARGLPEAAARENAL"
     gene            complement(3434..3718)
                     /locus_tag="VFL84_02970"
     CDS             complement(3434..3718)
                     /locus_tag="VFL84_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607613.1"
                     /translation="MTAASSSAWSGSTTIGDLPARPGVAFGTVCTGVAISPRSSSITV
                     TEAASTAMTAAARILRQSRRLRAAALSAGVSAGTRPGDAAAGSASKDFGT"
     gene            4294..4809
                     /locus_tag="VFL84_02975"
     CDS             4294..4809
                     /locus_tag="VFL84_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607614.1"
                     /translation="MDIDGGNLHANLALMGSQYFSGADFHEIAERVLSTWPENAEVEA
                     YVGAMFVLSGETERGRALVADAIQWTSKVPSGYYASSALAALRERQYEEALSFALRID
                     APDWPLGHIIVASAGALGGRADLAKRARERLIELEPAIARKLPDVLHRWRVEPVLAGE
                     LERGFAAAGQP"
     gene            complement(4814..>5035)
                     /locus_tag="VFL84_02980"
     CDS             complement(4814..>5035)
                     /locus_tag="VFL84_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607615.1"
                     /translation="SLPARLRDGPLRTMLTLQIKAGTLAAVAQNDRQRLERLVELLQL
                     AQMAAKEMRYFSLDLQKLIDDLTPSTKAE"
BASE COUNT          859 a         1679 c         1731 g          766 t
ORIGIN      
        1 ctcgaggacg ccgtcgcgga ggcgcggcgg ctcacgagct tcggggcgaa acgccgccag
       61 actcagttca tcggcaagct catgcgcaag ctcgacgagg actcggtcgc cgcgattcgc
      121 aaagcgttgc agcgcgactg accgccgccg aaattgctcc ttggccccgc ggccacaagt
      181 ccggtatcgt ggccgttcaa cgcaaggaga gtcagatggc gcagctacag tggacggcac
      241 cgcccgagat gaagatcgac gtcgcgaagg actacgcggt ctcgatcgag acgaatcgcg
      301 gcacgatcga gctcgcgctc tatccgaagc acgcgccgca gaccgtcaac aacttcgtgt
      361 tcctcgcggg gcaggggttc tacgacggcg tgaaattcca tcgcgtcatc gcagacttca
      421 tgatccagac gggcgatccg accggcaccg ggcgcggcgg ccccggctac aagttcggcg
      481 acgagaccaa gagcaatccg ctcaagcacg agcgcggcgt catctcgatg gcgaacgcgg
      541 ggccgaacac gaacggcagc cagttcttca tcacgcacgt gccgacgccg cacctgaacg
      601 gcaagcacac cgtgttcggc aaggtgacga aaggcctcga cgtcgtcgac gcgatccgtc
      661 agggcgacac gatggtcaag gtcaaggtcg ctgactaggc gcgctacatc ggcggcccgg
      721 tgccggcgtg acgcggcacg cgccagtatc cgtcaggcga gcagttgctg cacgcttccg
      781 tcgccggcct tcgagaacaa atcggtgaac acgccgcgga tccaacggct gccgggctcg
      841 cgatgaaacc gctcgtgcca gtgctgtgcg acttcgatgc ggggtaggcg tatcggcgtc
      901 gcgaaaacgc gcaggcgcag cgcgtcgctg agctcggagg cggtgccggc cggcaccgtc
      961 gcgacgacgt cggtccggct cgcggcatac gccgcggcca tgaaggtcgg cacgcggcag
     1021 acgatgtgct cctcaggtat ggcgcgctcg agcgcgcgct cgacgagctg gtgctcgtgg
     1081 cccgtgcgtg ccgtcgacac gagcacgtgc cgctcggcgg cgaacgcggc cagcgacggc
     1141 tttgcggtga tgcgtggatg gtcgcggcgc acgacgctga cgtagccgac cgaccacagc
     1201 gcctggcggc gaatgcgttt cgagcggctg cggaacgagc ccatcgcgaa atcgaggtga
     1261 cccgcctcga gcgccgcttc gagctcctcg aactcgatcg gcgtcacgcg caggcgcacg
     1321 cccggcgcgt gcgtctcgag atgcttgagc agcgcgggca gcaggtgcac gagccccgag
     1381 tcgaccacgc tgaacgagaa ggtgcgggtc gacgtcttcg gatcgaacgg ccggtgtcgc
     1441 gcacgcagca tcgtcgcgtc gtcgagcagc gcgtgcaccg catcggcgag ctcgaccgcc
     1501 ttggccgtcg gctccatgcg gtgaccggcg cgcacgaaca gcggatccgc gaaatagcgg
     1561 cgcaacttcg ccagcgtctt gctcaaagcg ggctgcgcga cgccgagcac ggccgcggcg
     1621 cgcgtcaggt tgcgctctcg cagtaacacc tcgaaagcgc gcagttgtcg aaggtcgagc
     1681 tccactgtat tcccgctggg aatcagaccc atagccaatt gcgcatagtg tacggagccg
     1741 cgcgagccta ggctatccgg accggcgtat gctcacgaaa agggacgtta tgacgcgaac
     1801 tatcaagctc acggccgcgg cgctgctttt cggagttgga ctgccggcgg cggcccatca
     1861 ctcgttcgcg atcttcgagc actcgaagac ctataccctc aaaggcacgg tgcagtcgtt
     1921 ccagtggacg aacccgcacg gctatatcga tctcgaggtc gccgacggcc cgcccggaat
     1981 cgatcactac accttggagt tgacgagcat caacatgctg cggcgcgcag ggtggaaatc
     2041 gagcgacgtc agcgtgggcg acaaagtcac cgcgatcgtc gcgccgctca tgaacggcca
     2101 gcatggcggt ttgctgctcg agctcaagat ggaggacggc cgcacgctcg tgccgccggt
     2161 cccggcgatc aacaccttca agaggacgga gtgagagcat gaaggcgccg actcgtcgca
     2221 tcgcgctcgc ggccgtggcc gcggcgacgc tcgccgtcag cgccgttgcc gccgcgcaga
     2281 gcggcgcacg gcccgacctc acgggctcgt gggaacggta ccgcgggccg gccggggacg
     2341 cgacgaatcc gccgcccgaa tcgcagccgc ccctcaaacc ggatctgctc gccgaatggc
     2401 agaaacgccg ccaggccgag cgcgaggccg acgcgcgcgg cgagccgctc gcgacgaact
     2461 acacgcactg cattcccgac ggcgtgccgt cgatgatgag cggtccgttc ccgttcgaga
     2521 tcgtccagag cgcttcgcag atcaacatcg cgcaagaggc gtacagccag gttcgccgca
     2581 tctacttggg caagccgcaa gtcacgctcg acgacatcga gctcgggttc tacggccgct
     2641 ccgtcggcga gtggcgcggc gatgagctct tggtcgatac gatcggcatc aaggactacg
     2701 tgcgctttcg cgacgtgccg cacacgccgc agatgcgcat caaggaacgc ttcaagctcg
     2761 tgcggccgga catcctctgg gacgagatca cgatcgagga tcccgcggtg ctcgagaagc
     2821 catggacggt gacgttcgcg taccggcgca tgccggacta cgagatcatc gagtacgtct
     2881 gcgaggacaa tcgcgagtac gccgacgaag aaggcgcgac gcggctgcgg ctgctcgagc
     2941 gtcaggaaag ccgctagcaa accggcgttc cgcctcgttc gcagggtcgt ttccgaaacc
     3001 gccctcggaa gcctacactg tcatttcagg aaaggtttcc gtttggggcg ccagcccggg
     3061 tgggtcccat tcgcgaggac agcgtattgc agggatcggc ggggtcagcg gagcccagcc
     3121 ccggtgaagt tcgcgccgcg ctagagcgcg ttctcgcgag ccgctgcttc gggcaggccg
     3181 cgcgctcgtc ggcgttcctg cgctacgtcg ccgagcaaac gctggccggt caaggcgacc
     3241 ggctcaaggg ttacacgatc gccgtcgagg tgttcggccg tccgccggac ttcgatgcgc
     3301 agaccgaccc gctcgtccgc gtcgaggccg gccgtttgcg ccgccggctg atcgagtact
     3361 acgccgacga aggaagcgcc gatcccgtgc gcctcgagtt gccgcgcggc agctactccg
     3421 tcgtcagcac ttatcacgtt ccgaaatctt tggaggcgga gcccgcggcc gcgtcgcccg
     3481 ggcgagtgcc ggcggagacg cccgccgaca acgccgcggc gcgcagccgg cggctctggc
     3541 gcagaatccg cgcggccgcc gtcatcgcgg tgctcgcggc ttcggtgacc gtgatgctgc
     3601 tcgagcgcgg cgagatagcg acccccgtgc acaccgtccc gaacgcgaca cccggcctcg
     3661 ccggcaggtc gccgatcgtc gtcgagccgc tccaagcgct cgacgacgcg gctgtcacgc
     3721 aagagattgc gtcggcattg accgaagagc tgttcctcat gctcgacggt ccggagcgtc
     3781 tcgtggtggc agccgaagcc ggtgggctcg caaacgcgcc gccgggctat gtcctgacgg
     3841 gcagcgtgcg cgagatggac ggcggcttca ggatcatggc tcgcgtcttg ctcggcgatt
     3901 ccggcacgca agtctggagc gaggcctacg acgagtcgct cgacgcgctg cggtcggcgc
     3961 cgggcgaacg ccgcatcgcg aggctgatag cgctcgccac ggagccctat gggcccgtgt
     4021 tcgaggtgga gctcgagcgc ctgcgcgcgc tgactgcgca cgagccggac gcgcgcgagt
     4081 gcgtgctgcg gtattacgaa tatcgccgcg tgctcggcgc cgccgagcat gcccgcgcgc
     4141 tcgactgctt cgagctcacg acggtgcgcg atcccgacaa cgccgaagtc tgggccggcc
     4201 tgtcgttact gacgacggac gcttgggcgc acgggcggtc acggcggcag cgacacgctg
     4261 ctcgtgcatg cgcgcgaggc ggcccgccgc gcaatggata tcgacggcgg caatctacac
     4321 gcgaacctcg cgctcatggg ctcgcaatac ttcagcggcg cggatttcca cgaaatagcg
     4381 gaacgcgtcc tctccacgtg gcccgagaat gcagaagtcg aagcctacgt cggcgcgatg
     4441 ttcgtgttgt ccggcgagac cgagcgcggc cgcgcgctcg tcgccgatgc gatccagtgg
     4501 acgtcgaagg tgccgagcgg ctattacgcg tcgagcgcgc tggcggcgtt gcgcgagcgc
     4561 caatacgaag aggcgctctc gttcgcgttg cgcatcgacg cgccggactg gccgctcggg
     4621 cacatcatcg tcgcgtctgc cggcgcgcta ggcgggcgcg cggatctcgc gaagcgcgcg
     4681 cgcgagcggc tcatcgagct cgagccggcg atcgcgagga agctgcccga cgtgctgcac
     4741 cgctggcgcg tcgagccggt gctcgccggc gagctcgagc gcgggttcgc ggcggctggg
     4801 caaccttaac tcgtcactcg gccttggtgc tcggagtcaa gtcgtcgatc agcttttgca
     4861 ggtcgagcga aaagtagcgc atttctttcg cggccatttg tgcaagctgc aagagctcga
     4921 cgagcctctc gaggcgttgc cggtcgtttt gggcgacggc ggcgagcgtc ccggccttga
     4981 tctgcagcgt cagcatcgtc cgcaaaggtc cgtcgcgcag gcgcgccggc aatga
//
LOCUS       DASSNN010000168         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_33678, whole genome shotgun sequence.
ACCESSION   DASSNN010000168 DASSNN010000000
VERSION     DASSNN010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33678"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFL84_02985"
     CDS             <1..606
                     /locus_tag="VFL84_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607616.1"
                     /translation="ELTAGRAHLTGLTVDNPPGYDTDYAVRIGTATVSLDIGSLAGDV
                     PVVKDIELDGALINAEQRGAASNLTDIQKHATADSSDASAGESGRIIVERFRLRNARV
                     LVTSEHLSSAEELPLEDVVVEHIGTASGGATYSEAAEAMLMPVIAAARAAAADRLRDV
                     AGTVVSQAAREELDEEEEGLREKTDEAREEINEKVEELLDR"
     gene            complement(572..1708)
                     /locus_tag="VFL84_02990"
     CDS             complement(572..1708)
                     /locus_tag="VFL84_02990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7607617.1"
                     /translation="MKRVKLCILVPAHWEAQMGGSQYQAKVLVDHLLAHYDVDIAYLT
                     TRADPNFKPDGYRIVPFSDRRGLRRYGSFFDVARLYRALARERPDIILQFVGSAHLGI
                     AALYARRHGCKLIWRVTNDPSVEPDETSWRKPHLKLERLFMSFGIRNATLILAQTDYQ
                     RARLEETFPNAPVQVLPNFHPAAPDCGRNGAVVKQVAWIANLKPLKNPGAFVRLARRF
                     AHRADVRFVMVGGTTDSEWTRQQLKEIAATENLDYRGPISQAAVNSLLEQTDLLVNTS
                     DHEGFSNTFIQAWMRRGPVVSLRVDPDRLLSRGGLGAVTGDEDALSRQVAALLDDPDR
                     CAAIGARARRYALEHHAESNIETLARLLRLPRWLSDLAAPRLSR"
     gene            complement(1705..>2432)
                     /locus_tag="VFL84_02995"
     CDS             complement(1705..>2432)
                     /locus_tag="VFL84_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET7607618.1"
                     /translation="RPLLATLDDYLDPILVAGCQRSGTTALARLLKRADGMIDHAFGH
                     DDELDGALLLAGYATRGTDGRHCFQTTYLNDRVGEYFEHGNFRLIWMLREPRSVVYSM
                     LHNWKRGALNRLFEACGAEVLAQNDPAPSRSITWLGPSRIAKACSSYVAKTAQTFELA
                     RRLEPRIAVVDYDELVAKKETMLPELCEFVGLPYEPRLAAGLHARSVDKREQLGARDA
                     AYVDWFCSAVYDRARAQRTISAE"
BASE COUNT          405 a          786 c          858 g          383 t
ORIGIN      
        1 gagctgacgg cgggccgcgc gcatttgacg gggctcacgg tcgacaaccc gccgggctac
       61 gacacggatt acgccgttcg gatcggcacg gcgacggtat cgctcgacat cggctccctg
      121 gccggcgacg tccccgtcgt caaggacatc gagctcgatg gcgcgctgat caacgccgag
      181 cagcgcggcg ccgcgagcaa cctcaccgat atccaaaagc atgcgacggc cgattcgagc
      241 gatgcgtcgg ccggagagtc gggccggatc atcgtcgagc ggttccgctt gcgcaacgcg
      301 cgcgtgctcg tcacatcgga gcacttgagc agtgcggaag agctaccgct tgaggacgtc
      361 gtcgtcgagc acatcggcac cgcgtccggc ggcgcaacgt actcggaagc cgccgaagcg
      421 atgttgatgc cggtcatcgc ggccgcgcgc gcggccgcgg ccgatcggct acgcgacgtc
      481 gcgggaaccg tcgtgtcgca ggcggcgcgc gaagagctcg acgaggaaga ggaagggctc
      541 cgcgagaaga ccgacgaggc tcgcgaggaa atcaacgaga aagtcgagga gctgctagat
      601 cgctgagcca gcgcgggaga cgcagcaagc gcgcgagcgt ctcgatgttc gactcggcgt
      661 ggtgctcgag cgcgtagcgg cgcgcgcgcg cgccgatcgc ggcgcagcgg tcgggatcgt
      721 cgagcagcgc cgcgacttgt ctcgacagcg cgtcctcgtc gccggtcacg gctccgagcc
      781 cgccgcggct caagaggcga tcgggatcga cgcgcagact cacgacggga ccgcgccgca
      841 tccacgcttg aatgaacgtg ttcgagaagc cttcgtgatc gctcgtattg acgagcaggt
      901 cggtctgctc gagcaggctg ttgacggccg cctgactaat cggaccgcgg tagtcgaggt
      961 tttcagtggc ggcgatctcc ttcagctgct gcctggtcca ttcgctgtcg gtcgtgccgc
     1021 cgaccatgac gaagcgcacg tcggctcggt gcgcgaaccg ccgcgcgagg cgaacgaagg
     1081 cgcccgggtt tttgagcggc ttcaagttgg cgatccaggc gacctgcttg accaccgcgc
     1141 cattgcgtcc gcagtcgggc gccgccgggt gaaagttcgg aagcacttgg acgggcgcgt
     1201 tcggaaacgt ctcctcgagg cgcgcgcgct ggtagtccgt ctgcgcgaga atcaacgtcg
     1261 cgttgcggat tccgaagctc atgaacagcc gctcgagctt caggtgcggc ttgcgccacg
     1321 acgtctcgtc cggctcgacg ctggggtcgt tcgtgacgcg ccagatcagc ttgcagccgt
     1381 ggcgccgcgc gtagagcgcg gctattccca aatgcgccga gccgacgaac tgcaggatga
     1441 tgtcggggcg ttcgcgcgcg agtgcgcgat agagccgcgc gacgtcgaag aacgagccgt
     1501 agcgccgcag gccgcggcga tccgagaacg gcacgatgcg gtaaccgtcc ggcttgaagt
     1561 tgggatcggc ccgcgtcgtg agataggcga tatcgacgtc gtaatgggcg agcaggtgat
     1621 cgacgagcac cttcgcttgg tactgcgagc cgcccatctg cgcctcccag tgagcgggca
     1681 cgaggatgca gagcttgact cgcttcattc cgccgagatc gttcgctgcg cgcgcgcgcg
     1741 gtcgtagacg gcggagcaga accagtcgac atacgccgcg tcgcgcgcgc cgagctgctc
     1801 gcgcttgtcg acgctccgtg cgtgcaaccc cgcggcgagc cgcggctcgt acggaaggcc
     1861 gacaaattcg cacagctcgg gcaacatcgt ttcctttttg gccacgagct cgtcgtagtc
     1921 gacgacggcg attcgcggct cgagccgccg cgcgagctcg aacgtttgcg cggtcttggc
     1981 gacgtaggac gagcacgctt tcgcgatgcg cgatggcccg agccaggtga tgctccggct
     2041 cggcgcgggg tcgttctgcg cgagcacctc ggcgccgcac gcctcgaaca gcctgttcag
     2101 cgcgccgcgc ttccagttgt gcagcatcga gtacacgacc gagcgcggct cgcgcagcat
     2161 ccagatcaac cggaagttgc cgtgctcgaa gtactcgccc acgcggtcat tcagatacgt
     2221 cgtctggaag caatggcggc cgtccgtgcc gcgggttgcg taaccggcca gcagcagcgc
     2281 gccgtcgagc tcgtcgtcgt ggccgaacgc gtgatcgatc atgccgtccg cgcgcttcag
     2341 caagcgcgcg agcgccgtcg tgccggaacg ctggcagccg gcgacgagga tcggatccag
     2401 gtagtcgtcg agcgtcgcga gcagcgggcg gt
//
LOCUS       DASSNN010000169         5732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1656856, whole genome shotgun sequence.
ACCESSION   DASSNN010000169 DASSNN010000000
VERSION     DASSNN010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656856"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..272)
                     /locus_tag="VFL84_03000"
     tRNA            complement(186..272)
                     /locus_tag="VFL84_03000"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(236..238),aa:Leu,seq:cag)
     gene            413..2605
                     /gene="rnr"
                     /locus_tag="VFL84_03005"
     CDS             413..2605
                     /gene="rnr"
                     /locus_tag="VFL84_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET7607619.1"
                     /translation="MPRGPKDGAAGERRYEHKIPTREQILAAMELAGQPLKLEALAPR
                     FGIRTEQHRRALEARMRAMVRDGQLLRNRADEYCLTGHLDVVTGTVLAHRDGFGFLRP
                     DDGSPDLYLEARQMQVLWDGDRVAARANETSRGREGHVVEILARGKQRIVGQFRRERG
                     IDFVLESGDSCAEVLIARGETRGAKAGDVVAVDVLEYPSKTSHAIGRIVEVLGRKDTP
                     GIDTDVAILAHGIPNEWPAEALREARSWSAEVPESAKQGREDLRSTPLVTIDGADAKD
                     FDDAVYCEPQGEGWRLLVAIADVSHYVRADSPLDKEARSRGTSVYFPDRVVPMLPEEL
                     SNGLCSLNPNVDRLCLVCEMHVTRAGEVTRSRFYDAVMRSAARLTYVDAAAMLVAARP
                     RGPHAELKPQLQHLNAVYEAFAAARKQRGAIDFDLPESKIELDERGQVKSVRTAERLV
                     THKIIEECMIAANVEAAKRIRKGRIPGLYRVHEGPDEDRLEELVLFLRTFGHKVNPSK
                     LSPKEINRVLESVVGKPEEELVETVVLRSMKQARYQPNNVGHFGLALGAYAHFTSPIR
                     RYPDLLVHRAIKHLSDKRSPKGFRYGLEEMERLGEHTSRTERRADEATREVAEKLKCV
                     YLKERVGDTFDVVISSVVPFGLFVRLPEIQADGLVHVTALPRDYYHKDATGTVLRGER
                     SGREYRLTETLRVRLVAVNVEERKVDFVPVENDDEPARKPSRPRRGRG"
     gene            2598..3341
                     /gene="rlmB"
                     /locus_tag="VFL84_03010"
     CDS             2598..3341
                     /gene="rlmB"
                     /locus_tag="VFL84_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004234494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET7607620.1"
                     /translation="MASRRQHLYGLHAVRAVLERRPETVLAAKILRDAGGKLAELERA
                     LAARGIALERVARADLDRLAEGGVHQGIVVEVAAAPEFSIGDFEELVVARGRALRLLV
                     LDGVEDPRNLGACLRTADAAGVDAVVVPRDHSAKLTATAVKTSTGAAETVPVFRAPNL
                     ARSLSWLKEAGVWVVGADAEAPRSLYQTKLAPPVALVLGGEGRGVRRLTRDLCDELVY
                     IPMAGSVESLNVSVATGIVLFELLRQAPK"
     gene            3477..3926
                     /gene="rpsF"
                     /locus_tag="VFL84_03015"
     CDS             3477..3926
                     /gene="rpsF"
                     /locus_tag="VFL84_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET7607621.1"
                     /translation="MRHYEVVFLVHPDQSEQVPAMMERYRNMIEGAGGKIHRQEDWGR
                     RQLAHSIAKVHKAHYLLLNIECDKPTLDELVGAFRFSDAVLRYLVISRDEAVTEPSFM
                     AKAREEEEEREAQSAQRDRPPRERPRPAADFNSPDATFGEEPAEERE"
     gene            3945..4169
                     /gene="rpsR"
                     /locus_tag="VFL84_03020"
     CDS             3945..4169
                     /gene="rpsR"
                     /locus_tag="VFL84_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020584246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET7607622.1"
                     /translation="MARYFRRKKFCRFTAEGVKEIDYKDLATLKAYITETGKIVPSRI
                     TGTSAPYQRQLATAIKRARYLALLPYTDQH"
     gene            4220..4681
                     /gene="rplI"
                     /locus_tag="VFL84_03025"
     CDS             4220..4681
                     /gene="rplI"
                     /locus_tag="VFL84_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019640985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET7607623.1"
                     /translation="MEVILLQKVDNVGGIGDLVRVKSGYARNYLIPQGKATLATAENK
                     AKFESRRAELEAKAAAELAAAQDRAKKLEGVTLKIEMQAGAEGKLFGSVGTVDIAEAI
                     GKHGVEVERSEIRLPEGPLRVVGDHQIELHLHTDVNVELKVVIEAAEPENA"
     gene            4717..>5732
                     /locus_tag="VFL84_03030"
     CDS             4717..>5732
                     /locus_tag="VFL84_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HET7607624.1"
                     /translation="MAESAATELARRATERLRLPPHSVEAEQSLLGGLMLDQRAWDQI
                     ADLVAADDLYRADHRLIFSAVAALVERNQPPDAVTVSEHLQRLGQLEAAGGLPYLARL
                     VEDTPSAANIRAYAGIVRDHAMLRKLIEIGGDIAASAHATEGLTASEIVDRAEQLVFD
                     IAERGSRRGSGFVSLKQILPQTIDRLDFLSHSTSEITGVSTGFAEMDKWTSGLQRGEL
                     VIIAGRPSMGKCVVSGSRILNPETGALETIDDCVRDRRGNVVSLDATGRLQAAKASDF
                     VDDGVKPVFELTTALGRRVLVTAPHPFLTPTGWQPLASLRPGMCVAVPRALPFFGNRR
                     LADSL"
BASE COUNT          990 a         1883 c         1976 g          883 t
ORIGIN      
        1 tgtgtcgcac accctcgacg tcggcgaccc aaccgccgcg aacgtcgggc tgtgcgaacg
       61 ccggcgcggc gcaacctgag agcagggcca cgaaccagcg gatcgcggtt ctctttctca
      121 tcatgggtcc cccgtcgaga ccggccgccg aagtcgtcgg tgttgcgagt tgccgagaag
      181 agccgtggtg cccaggagag gacttgaacc tccacgagtg ttaccccact ggcacctgaa
      241 accagcgcgt ctaccaattc cgccacctgg gcatagggag cagaagggcg acgaatgtac
      301 tgacaccctc cggggctgtc aaaccgctcg gggccaacaa gcgcgcggat gccggctcgg
      361 ctaagatgct ttaaatgttc aatcgattgc tcgagccgcg aggctgctcc tgatgccgcg
      421 cggtccgaaa gacggcgccg ccggcgaacg ccgttacgag cacaaaatcc ccactcgaga
      481 gcagattctc gcggcgatgg agctggccgg gcagccgctc aagctcgagg cgctggcgcc
      541 gcggttcggc atccgcaccg agcagcatcg ccgggcgctc gaggcgcgca tgcgcgcgat
      601 ggtgcgcgac ggccagctgt tgcgaaaccg cgccgacgag tactgcctca cgggtcacct
      661 cgacgtcgtc acgggcaccg tgctcgcgca ccgcgacggg ttcggcttct tgcggccgga
      721 tgacggctct cccgacctct atctcgaggc gcggcaaatg caggttctct gggacggcga
      781 tcgcgtcgcc gcgcgcgcga acgagacgag ccgcggccgc gagggccacg tcgtcgagat
      841 actggcgcgc ggcaagcagc gcatcgtcgg gcaatttcgc cgcgagcgcg gtatcgactt
      901 cgtgctcgag agcggcgatt cgtgcgccga ggtgctgatc gcgcgcggcg agacgcgcgg
      961 cgcgaaggcc ggcgacgtgg ttgccgtcga cgtgctcgag tatccgagca agacgagcca
     1021 tgcaatcggc cgaatcgtcg aggtcctcgg ccgcaaggac acgcccggca tcgacaccga
     1081 cgtcgcgatc ctcgcgcacg gcattcccaa cgaatggccg gccgaggcgc tcagggaggc
     1141 gcggagctgg tccgccgagg ttcccgagag cgcgaagcag ggccgcgaag acttgcgcag
     1201 cacgccgctc gtgacgatcg acggcgccga cgcgaaagac ttcgacgacg ccgtatattg
     1261 cgagccgcag ggggaaggct ggcggctgct cgtcgcgatc gcggacgtca gccactacgt
     1321 gcgcgccgat tcgccgctcg acaaagaagc ccgctcgcgc ggcacgtcgg tctattttcc
     1381 cgaccgcgtc gtgccgatgc tgcccgagga gctgtcgaac ggcctctgct cgctgaatcc
     1441 aaacgtcgat cggctctgcc tcgtctgcga gatgcacgtg acacgggccg gcgaagtgac
     1501 gcgctcgcgg ttctacgacg ccgtcatgcg ctcggccgcg cgcctcacct atgtcgacgc
     1561 ggccgcgatg ctggtcgcgg cgcggccacg cggcccgcac gccgagctca agccgcagct
     1621 gcagcacttg aacgccgtct acgaggcctt cgcggcggcg cgcaagcagc gcggcgcgat
     1681 cgacttcgat ttgccggaaa gcaagatcga gctcgacgag cgcggccagg tcaagagcgt
     1741 gcgcaccgcc gagcgcctcg tcacgcacaa gatcatcgag gagtgcatga ttgcggcgaa
     1801 cgtcgaggcg gcgaagcgca tccgcaaagg ccgcatcccg ggcctgtatc gcgtgcacga
     1861 agggcccgac gaggatcgcc tcgaggagct cgtgctgttc ctgcgcacgt tcggccacaa
     1921 ggtcaatccg tcgaagctct cgccgaaaga aatcaatcgc gtgctagaga gcgtcgtggg
     1981 caagccggag gaagagctcg tcgagaccgt cgtgctgcgc tcgatgaagc aagcccgtta
     2041 tcagccgaac aacgtcggcc acttcggctt ggcgctcggc gcgtacgcgc acttcacgtc
     2101 gccgatcagg cgctacccgg acctgctcgt gcatcgcgcc atcaagcact tgagcgacaa
     2161 gcgctcgccg aaaggttttc gttacggcct cgaggagatg gagcggctcg gcgagcacac
     2221 ttcgcgcacg gagcggcgcg ccgacgaggc gacgcgcgag gtcgccgaga agttgaagtg
     2281 cgtgtattta aaggagcgcg tcggcgatac gttcgacgtc gtcatcagca gcgtcgtgcc
     2341 gttcggcctg ttcgtgcggc tgcccgagat tcaagccgac gggctcgtgc acgtcacggc
     2401 gctgccgcgg gactactacc acaaggatgc gaccggaacc gtgcttcgag gagagcgctc
     2461 ggggcgcgag tatcggctga ccgagacgct gcgcgtccgg ctcgtggccg tcaacgtcga
     2521 agagcgcaaa gtggatttcg ttcccgtcga gaacgacgac gagcctgcgc ggaagccgag
     2581 ccgcccgcga cgcggccgtg gctagccgcc gccagcacct gtacgggctg cacgcggttc
     2641 gcgccgtgct cgagcgccgt cccgagacgg tgctcgcggc caaaatcttg cgcgatgcgg
     2701 gcggcaagct tgcggagctc gagcgcgcgc tcgcggcgcg cggtattgca ctcgagcgcg
     2761 tcgcgcgcgc ggatctcgac cgcctcgccg agggcggcgt tcatcaaggc atcgtcgtcg
     2821 aagtcgcggc ggcgcctgag ttctcgatcg gcgacttcga ggagctcgtc gtcgcgcgcg
     2881 gccgcgcttt gcggctgctc gtgctcgacg gcgtcgagga tccgcgcaac ctcggcgcgt
     2941 gcttgcgcac ggccgatgcg gccggcgtcg acgccgtcgt cgtgccgagg gaccactccg
     3001 cgaagctcac tgccacggcg gtcaagacgt cgaccggcgc ggcggagacc gtgccggtct
     3061 ttcgcgcgcc gaacctcgcg cgctcgctct cctggctcaa ggaggcggga gtctgggtcg
     3121 tcggcgcgga cgcggaggcg ccgcgctcgc tctatcaaac caagctcgcg ccgccggtcg
     3181 cgctcgtcct gggcggcgag ggccgcggcg tgcggcggct gacgcgcgac ctctgcgacg
     3241 agctcgtcta tatcccgatg gcgggctccg tcgaaagcct gaacgtctcg gtcgccacgg
     3301 gaatcgtgct gttcgagctg ctgcgccagg cgcccaaata gggccttcgc gagcgatgcc
     3361 ggcttgagcg gcccgggcct actcgggtag gatggcggcc ccccggaacc gaacaatcgg
     3421 ggactccttg cctcaccgcg ttgtcgggag gctgttcaac ccatagggag atctcattga
     3481 gacactacga agtcgtgttt ctggtccatc ctgaccagag cgagcaggtt cccgcgatga
     3541 tggagcgtta tcgcaacatg atcgagggcg ccggcggcaa gattcatcgc caggaagact
     3601 ggggccgccg tcagctcgcg cattcgatcg cgaaagtcca caaagcgcac tacttgttgc
     3661 tgaacatcga gtgcgacaag ccgacgctcg acgagctcgt cggcgcgttc cgcttcagcg
     3721 acgccgtgtt gcgctatctc gtcatcagcc gcgacgaggc cgtcacggag ccttcgttca
     3781 tggcgaaggc gcgcgaggag gaagaggagc gcgaggcgca atcggcgcag cgcgatcggc
     3841 ccccgcggga gcggccgcgc ccggccgcgg atttcaatag tcccgatgcg acgttcggcg
     3901 aggaacccgc cgaagagcgc gaataggcta agaggatcac ttacatggct cgttattttc
     3961 gacgcaagaa gttctgccgc ttcacggccg aaggcgtgaa ggagatcgat tacaaggatc
     4021 tcgcgacgct gaaggcctac atcaccgaga ccggcaagat cgttccgagc cggatcacag
     4081 ggacgagcgc gccgtatcag cgccagctcg cgacggcgat caagcgcgcg cgttatctcg
     4141 cgctcctgcc gtacacggat cagcattgag caattgagcg cccggacgcg gcgacgcgcc
     4201 ggcgcatgag agtcgagaga tggaagtcat attgctgcaa aaagtcgaca atgtcggcgg
     4261 catcggcgat ctcgttcgag taaagtccgg ctacgcgcgg aattatttga ttccgcaggg
     4321 caaggcgacg ctcgcgacgg ccgagaacaa ggcgaagttc gagtcgcgcc gtgcggagct
     4381 cgaggcgaag gccgccgcgg agctcgcggc cgcgcaagac cgcgcgaaga agctcgaggg
     4441 cgtcacgctc aagatcgaga tgcaggccgg cgccgaaggc aagctgttcg gctcggtcgg
     4501 cacggtggat atcgccgagg cgatcgggaa gcacggcgtc gaggtcgagc gcagtgaaat
     4561 tcggctgcct gaggggccgc tgcgtgtcgt cggcgatcat cagatcgagc tgcacttgca
     4621 caccgacgtc aacgtcgagc tcaaggtcgt gatcgaggcc gcggagccgg agaacgctta
     4681 acgagaggcg cttcaagcgc caacagaggg ggcaggatgg ccgaaagcgc agcaaccgag
     4741 ctcgcccgca gggctacgga acgcctccgg ttgccgccgc attccgtcga agccgagcag
     4801 tccctgctcg gcggcctcat gctcgatcag cgcgcgtggg accagatcgc ggatctcgtc
     4861 gcggccgatg acctctatcg tgccgatcac cgtctgatct tctcggccgt cgccgcgctc
     4921 gtcgagcgca atcagccgcc cgacgcggtc accgtcagcg agcatttgca acgcctcggc
     4981 cagctcgagg ccgccggcgg gttgccgtat ctcgcgcgtc tcgtcgagga cacgccgagc
     5041 gcggcgaaca tccgcgccta tgcaggcatc gttcgcgatc acgcgatgct gcgcaagctc
     5101 atcgagatcg gcggggacat cgcagccagc gcgcatgcga ccgagggcct tacggcttcc
     5161 gagatcgtcg atcgcgccga gcagctcgtg ttcgacatcg cggagcgcgg ctcgcgccgc
     5221 gggtcgggct tcgtctcgct gaagcaaatc ctgccgcaga cgatcgaccg cctcgatttc
     5281 ctgagccact cgacgagcga gatcacgggc gtttcgaccg gcttcgccga gatggacaag
     5341 tggacgtccg gcctgcagcg cggcgagctc gtgatcatcg ccgggcggcc atcgatgggg
     5401 aagtgcgtgg tctcgggtag caggatcttg aatcccgaga cgggcgcgct cgagacgatc
     5461 gacgactgcg tgcgcgaccg gcgcggcaac gtcgtgtcgc tcgatgcgac cgggcggctg
     5521 caagcggcca aggcctccga cttcgtcgac gacggcgtga agcctgtgtt cgagctgacg
     5581 acggcgctcg gccgtcgcgt gctcgtcact gctccgcatc cgttcttgac gccgacgggg
     5641 tggcaaccgc tcgcgtcgct gcgcccgggt atgtgcgtgg cggtcccgcg tgcgctgccg
     5701 ttcttcggca accggcggct ggccgactca ct
//
LOCUS       DASSNN010000170         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_34383, whole genome shotgun sequence.
ACCESSION   DASSNN010000170 DASSNN010000000
VERSION     DASSNN010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34383"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2362
                     /locus_tag="VFL84_03035"
     CDS             <1..2362
                     /locus_tag="VFL84_03035"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET7607625.1"
                     /translation="LAAAGCQPSDEKLAKRYEGVVETYCLECHDAAGREAGLSLEDVD
                     LGNVAAHPELFETVARKLRGRQMPPPGGPRPDLETYDGFAGYLERRLDEAALASPEPG
                     KASIHRLNRTEYGNAVRDLLALDIDASEYLPADDEGYGFDNIADVLRVSPSLLEQYLA
                     ASAKIAALAVGDPEAPAVTAVYRAPSDLAQAVHVEGMPLGTRGGIKIRHYFPLDAEYE
                     FDVFLLRNIVGYMTGLEWAHELEIAVDGERVFVAQVGGEADNAMSDKNMSAAANEIDE
                     RLRTRAFVTAGPHDVTVAFLERSAAETHEPLELHTRNLDLQDMNGLPVVDYVNVRGPF
                     NPVGPGDTPSRERIFTCRPADDATARVCATEILSTLARRAYRRQPTEQDVATLLSFYD
                     SGSARAGFDAGIESALRVILTSPKFLFRDEPDPAGVAPGSLYALDDTALASRLAFFLW
                     SSPPDDELLDLARQGKLSDEKVFEQQVDRMLADERATALVENFAGQWLFLRNLQSTHP
                     DTEEFPDFDDNLRRSMRRETELLFEHVMREDLPVNELLTADYTFVNERLARHYGMPEI
                     YGSNFRKVPVADEARRGLLGHASILTVTSYPNRTSPVLRGKWVLENVLGTPPPPPLPD
                     VPALPDNETGKVARTLRERLAEHRANPICATCHDVMDPIGLGLENFDAVGKWRTREPG
                     GEIDAHGQLANGTPISGAAELREALTADPEQFARVFTSKLLTYALGRGLEAYDMPTVR
                     KIVREAEDDDYRFGAIVKGIVNSVPFRMRRAQSPDAPPQPTVASTQ"
     gene            2437..>2667
                     /locus_tag="VFL84_03040"
     CDS             2437..>2667
                     /locus_tag="VFL84_03040"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HET7607626.1"
                     /translation="MALIMELSKSEQRVLDVLWSESPLTVGQVIERLQKASDWHENTI
                     KTLLTRLVQKKAVARRKDGGRFFYRPLVSRDAI"
BASE COUNT          452 a          925 c          901 g          389 t
ORIGIN      
        1 gctcgcggcg gcgggttgcc agccctccga cgagaagctc gcgaagcggt atgagggcgt
       61 cgtcgagacc tattgcctcg aatgccatga cgccgccggc cgagaggccg ggctatcgct
      121 cgaggacgtg gacttaggca acgtcgcggc gcacccggag ctgttcgaga ccgtggcccg
      181 caagctgcgc ggacggcaga tgccgccgcc gggcgggccg cgccccgatc tcgagactta
      241 cgacggcttc gccggctacc tcgagcggcg cctggacgaa gccgcgcttg cgtcgccgga
      301 gccgggcaag gcgtcgatcc accggttgaa ccgcaccgaa tacggcaacg ccgtgcgcga
      361 cctgcttgcg ctcgacatcg atgcctccga gtacttgccg gccgacgacg aaggctacgg
      421 gttcgacaac atcgccgacg tcttgcgcgt atcgccgtcg ctgctcgagc agtatctcgc
      481 ggcctcggcg aagatcgcgg cgctcgcggt cggcgatccg gaggcccccg ccgtcacggc
      541 cgtgtaccgc gcgccgtcgg acctcgcgca agccgtgcac gtcgagggaa tgccgctcgg
      601 cacgcgcggc ggcatcaaga ttcgccacta cttcccgctc gacgccgaat acgagttcga
      661 cgtgttcctg ctgcgcaaca tcgtcggcta catgacgggg ctcgagtggg cgcacgagct
      721 cgagatcgcc gtcgacggcg agcgcgtgtt cgtcgcgcag gtcggcggcg aagccgacaa
      781 cgcgatgtcg gacaagaaca tgtcggccgc cgcgaacgag atcgacgagc gtttgcgcac
      841 gcgcgcgttc gtgacggccg ggccgcacga cgtgacggtc gcgttcctcg agcgcagcgc
      901 cgccgagacg cacgagccgc tcgagctgca cacgcgcaac ctcgatctgc aagacatgaa
      961 cgggttgccc gtcgtcgact acgtcaacgt gcgcgggccg ttcaatcccg tcggccccgg
     1021 cgacacgccg agccgcgagc gcatcttcac gtgccggccc gccgacgacg cgaccgcgcg
     1081 tgtctgcgcg acggagatcc tctcgacgct ggcgcggcgt gcctatcgcc ggcaaccgac
     1141 cgagcaggat gtcgcgacgc tgctctcctt ctacgactcc gggtccgcac gcgccggctt
     1201 cgacgcaggc atagagtccg cgctgcgcgt gatcctgacg agcccgaagt tcctgttccg
     1261 cgacgagccg gatccggccg gcgtggcgcc gggctcgctg tacgcgctcg acgacacggc
     1321 gctcgcgtcg cggctcgcgt tcttcctgtg gagctcgccg cccgacgacg agctgctcga
     1381 cctcgcgagg caaggcaagc tcagcgacga gaaggtcttc gagcagcagg tagaccgcat
     1441 gctcgccgac gagcgcgcga cggcgctcgt cgagaacttc gcgggccagt ggctgttctt
     1501 gcgcaatctg cagagcacgc acccggacac cgaggaattt ccggacttcg acgacaacct
     1561 gcggcgctcg atgcggcgcg agaccgagct cttgttcgag cacgtcatgc gcgaggacct
     1621 gcccgtcaac gagctgctga cggccgacta cacgttcgtc aacgagcgct tggcgaggca
     1681 ctacggaatg ccggagatct acggcagcaa cttccgcaag gtacccgtcg ccgacgaggc
     1741 ccgccgcggg ctgctcggcc acgccagcat cttgaccgtc acgtcgtatc cgaaccgcac
     1801 ttcgccggtg ctgcgcggca aatgggtcct cgagaacgtg ctcggcacgc cgccgcctcc
     1861 gccgctgccg gacgtgccgg cgctgcccga caacgagacg ggcaaggtcg cgcgcacgct
     1921 gcgcgagcgg ctcgcggagc atcgcgcgaa tccgatctgc gcgacgtgcc acgacgtcat
     1981 ggatccgatc gggctcgggc tcgagaactt cgacgccgtc ggcaagtggc gtacgcgcga
     2041 gccgggcggc gaaatcgacg cgcacggcca gctcgcgaac ggcacgccga tctcgggcgc
     2101 cgcggagctg cgcgaagcgc tgacggccga tcccgagcag ttcgcgcgcg tgttcacgtc
     2161 gaagctgctc acgtacgcgc tcggccgcgg gctcgaggcc tacgacatgc ccactgttcg
     2221 taagatcgtg cgcgaggccg aggacgacga ctatcgcttc ggagcgatcg tgaaaggcat
     2281 cgtgaacagc gtgccgttcc gcatgcgccg cgcgcagtcg cccgacgcgc cgccgcagcc
     2341 gaccgtcgcc agcacccagt aagccgaagg cgtttcggcg ccgggttgac acgtccgccg
     2401 accaagacta cagtcgtagt caaggagact aaagcaatgg ctttaatcat ggagctgtcg
     2461 aagtcggagc agagagtgct ggacgtgctc tggagcgaga gcccgctcac cgtagggcag
     2521 gtcatcgagc ggctgcagaa ggcctccgat tggcacgaga acacgatcaa gacgctgctg
     2581 acgcggctcg ttcaaaaaaa ggccgttgcg cgtcgtaagg acggcggccg cttcttctac
     2641 cggccgctcg tatcgcgcga cgcgatc
//
LOCUS       DASSNN010000171         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1278423, whole genome shotgun sequence.
ACCESSION   DASSNN010000171 DASSNN010000000
VERSION     DASSNN010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278423"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1044)
                     /locus_tag="VFL84_03045"
     CDS             complement(208..1044)
                     /locus_tag="VFL84_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607627.1"
                     /translation="MAVNYGTHEHRFAATAWFGTEGMKVSWGGVFAGVLVAMGVSMLL
                     AALGVAIGISAVDPGETNPGTVGIGAAIWGGVQLLIALFIGGMVATRVGAIIDRTTGF
                     FEGVLVWVVSLILMAYLAGSGIASVASGAFSLLGGATQTFSAVVQGQGGTGNIDVSGT
                     VDEMAEQLRSPELVNRVASVTGLSVNEVRNTLNDTAARVEANRNNPTQAAQEASRGIA
                     TLFDRARASGALAAQADEIKPEAASTAWLTFLALLLSLAAAVAGSMLGRSKAADAVAA
                     GG"
     gene            complement(1073..1687)
                     /locus_tag="VFL84_03050"
     CDS             complement(1073..1687)
                     /locus_tag="VFL84_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607628.1"
                     /translation="MCRWIRWRAILCSALSLAAAGTASAQDTGRRRVTTIDHAVEVYI
                     SNDAMEAQYVRMLDLGELGPTQARGGFLYNEDRDLVAIADLLVDVGDEIDVRGLELRV
                     GTRVYGAFLAPEDQDVFSIGLGGEAQYFLGSRRNASVTLGLFYGPDIVTFGTADHAMD
                     VSLRLMTRMRNGTDVFVGYRTLEFGVDPQRDVDDNLHLGFRRSF"
     gene            1917..>2247
                     /locus_tag="VFL84_03055"
     CDS             1917..>2247
                     /locus_tag="VFL84_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607629.1"
                     /translation="MLFAAPRIAACQPYVSGQLGLASTDWPRDAPLNGRIDDNAAGYG
                     IDFGIGFGKRWAFEVGAYGYGSFDATGTPCADGSACLPIVTEVGGNDITIYKAALAPR
                     FIVGKVRL"
BASE COUNT          381 a          786 c          707 g          373 t
ORIGIN      
        1 catcaaggcc cacggccggc tcgcttgcgg taggcggaac atcgccccaa ccttgctcca
       61 tccgcgacgc cttctagtag acgcaagcgg cagaggacag gtcaaagcga ctgcaatcgg
      121 cgacagccgc cgacgcagcc tttcggccct cgagcccggc gcccgcggcg ccgggcgtcg
      181 ggcccgcgca gcgatttacg ccccgattta cccgccggct gcgacggcgt cggcggcttt
      241 gctgcggccg agcatcgatc ctgcgacggc cgccgcgaga gagaggagca gcgcgaggaa
      301 cgtcagccat gccgtcgacg cggcttccgg cttgatctcg tcggcttgcg cggcaagcgc
      361 cccgctcgcg cgcgcgcggt cgaacagcgt cgcgatgccg cggctcgcct cctgggcagc
      421 ctgcgtcgga ttgttccggt tcgcctcgac gcgtgcggcc gtgtcgttca acgtattacg
      481 gacctcgttg accgacaggc ccgtgacgga cgcaacacga ttcacgagct cggggctgcg
      541 gagctgctcg gccatctcat cgacggtccc ggacacgtcg atgttgccgg tcccgccttg
      601 cccctggacg actgcgctga aggtctgcgt ggcgccgccg agcaggctga aggcgccgct
      661 ggccacgctc gcgatgccgc tgccggcgag atacgccatc aggatcaggc tgacgaccca
      721 aacgagcacg ccttcgaaaa acccggtcgt gcggtcgatg atcgcgccga cgcgcgtcgc
      781 gaccatgccg ccgatgaaca acgcgatcag cagctggaca ccgccccaga tcgcggcgcc
      841 gatgccgacg gtgccggggt tcgtctcgcc ggggtcgacg gccgatatgc cgatcgcgac
      901 accgagcgcc gcgagcagca tgctgacgcc catcgcgacg agcacgcccg cgaacactcc
      961 gccccacgat accttcatcc cttccgtgcc gaaccacgcg gtcgccgcga atcgatgctc
     1021 gtgtgttcca tagttgacgg ccatgtcgaa tctccttcgt caaacggttc gatcaaaagc
     1081 ttcgccgaaa gcccaagtgc aagttgtcgt cgacgtcgcg ctgcggatcg acaccgaact
     1141 cgagcgtgcg ataaccgacg aacacgtcgg tgccgttgcg cattctcgtc atgagccgca
     1201 agctcacgtc catggcgtga tcggccgtgc cgaacgtcac gatgtcgggt ccgtagaaca
     1261 gacccaacgt cacggacgcg ttgcggcgcg agccgaggaa atactgcgcc tcgccgccga
     1321 gaccgatgct gaagacgtcc tgatcttcgg gcgccaagaa cgctccgtag acgcgcgtgc
     1381 ccacgcgcag ctcgagcccg cgcacatcga tctcgtcgcc gacgtcgacg agcaagtccg
     1441 cgatcgccac gaggtcgcgg tcttcgttgt agagaaaacc gcctcttgcc tgagtggggc
     1501 ccagctcgcc gagatcgagc atgcgcacgt actgggcctc catcgcgtcg ttgctgatgt
     1561 agacctcgac ggcgtggtcg atcgtcgtta cgcgtctgcg gccggtgtct tgcgccgacg
     1621 cggttcccgc cgcggccaac gacaacgccg agcaaagaat cgctcgccac cgaatccaac
     1681 gacacatttt cgcaaccact taagcctcct tgctcgagtg agcgcaacgc cgaagcaaca
     1741 atcctgtctg gccgcgattg aggcaagttg cgtgccatgt gctcgcgaag ttgcaggatc
     1801 gccttccaat ccttgcaacc gcgcgccgag gcgcgcgctg ctcacgcgct gcccgagcat
     1861 tggctcgaat cttgcttcgg ccacgcatgc gtgctctcac cgttgttgcg gctctgttgc
     1921 tcttcgcagc gccgcgcatc gccgcttgcc aaccgtacgt ctcggggcag ctcggcctgg
     1981 caagcaccga ttggccgcgc gacgcgcccc tgaacggccg catcgacgac aacgcggccg
     2041 gttacggcat cgactttgga atcggctttg gaaaacgctg ggcgttcgag gtcggcgcgt
     2101 atggctacgg cagcttcgac gcgaccggca cgccctgcgc ggacggcagc gcgtgcctgc
     2161 ctatcgtcac ggaggtcggc ggcaacgaca tcacgatcta caaggcggcg ctcgcgccgc
     2221 gtttcatcgt cggcaaggtg cgtttgt
//
LOCUS       DASSNN010000172         3829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1043930, whole genome shotgun sequence.
ACCESSION   DASSNN010000172 DASSNN010000000
VERSION     DASSNN010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043930"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1849)
                     /gene="shc"
                     /locus_tag="VFL84_03060"
     CDS             complement(<1..1849)
                     /gene="shc"
                     /locus_tag="VFL84_03060"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HET7607630.1"
                     /translation="MLVSAPRALDTPAEQVEATIAKAIAWLDRDQTPEGFWVGMLESN
                     SCMEAEWLLAMHFLGRKHPREQDLVATLLNAQRPDGSWEAYYDAPQGDINGTVECYAA
                     LRSVGFDPADEPLVRARRWILEHGGLRGIRVFTRYWLALLGEWPWSATPNLPPEVIAN
                     PRWFPFNIYNFASWARATLLPLAVLSARRAVRPLPPGRRLDELFPEGRERMDYSLPRR
                     GPWLSWRRLFLLSDRVLHWYQQLGFTPFRETAINACLGWIVRHQDADGAWGGIQPPWI
                     YSLMALNVEGYRLDHPVMTAGLAALDSHWSYERGGTLHIQASESPVWDTLLALLALQD
                     CEREFTPAMTRALDWVLANEVRYHGDWTRKVKGVEPSGWAFERANLHYPDIDDTAVAL
                     IVLARLPRHLLDEPPIRATIDRALGWTLAMQSSSGGWAAFDKDNDRPIITKIPFCDFG
                     EALDPPSADVTAHVLEALGLLGFTLDHPAVARGFKFIRSEQEADGSWFGRWGVNHIYG
                     TAAVLPALAAIGEDMAQPYVRAAAEWLVAHQNADGGWGETCASYMDDALRGRGPSTAS
                     QTAWAILALVATNDRAYERAIRRGLGFLIDTQREDGTWDEPYYTGSGFPG"
     gene            2050..2367
                     /locus_tag="VFL84_03065"
     CDS             2050..2367
                     /locus_tag="VFL84_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607631.1"
                     /translation="MPTAGGQRLKLRSAEFRDYVERVFREQNRVADALAFALEAPGSP
                     DAELAAAEQELLAACAGVNELATARRDQRRLGLRSSVRAAKSVPDCERAALAAEARLS
                     RKE"
     gene            complement(2371..2862)
                     /locus_tag="VFL84_03070"
     CDS             complement(2371..2862)
                     /locus_tag="VFL84_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HET7607632.1"
                     /translation="MLTMLAAVAAATTGCAGPRVAPAAEAPRRMTVEPRVADEVVGGA
                     IADVAMGMVGTRYRFGGMDPLEGFDCSGLVYYAYGQAGYRVPRTSQELFRAARKISVG
                     DADPGDLMFFQDQTKLSHVGIYLGDGMFVHAPASGQNVAVASLDSPYYREHLVAVGRL
                     LPD"
     gene            complement(3013..>3829)
                     /locus_tag="VFL84_03075"
     CDS             complement(3013..>3829)
                     /locus_tag="VFL84_03075"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET7607633.1"
                     /translation="GLIVEGTAGNTGIGLALVGNSRGLRTLILMPNTQSQEKKDTLRL
                     CGAELREVPAVPYKDPNNYVHIAARTAKELEATEQHGVLYANQWDNPANRDGHTASTG
                     PEIWEQTNGSIDGFICAVGTGGTLAGVSAFLKHKNRNIVTAAADPMGAAIYSWIKTGE
                     LKGQGSSISEGIGQGRVTGNLDGALIDDAFQIPDTEMLPVLYDLLQNEGLLLGGSSGI
                     NVAGAIRLARQLGPGKTIVTVLADSGSRYQSKLFNPEFLKAKDLPLPPWLAKR"
BASE COUNT          603 a         1312 c         1302 g          612 t
ORIGIN      
        1 accccgggaa gcccgagccg gtgtagtagg gctcgtccca ggtcccgtcc tcgcgctgcg
       61 tgtcgatcaa gaacccgagc ccacggcgga tcgcgcgctc gtaggcgcgg tcgttcgtcg
      121 caaccagcgc gagtatcgcc cacgccgttt gcgacgccgt gctcgggccg cggccgcgca
      181 gagcatcgtc catgtacgac gcgcacgtct cgccccagcc gccgtcggcg ttctgatgcg
      241 caacgagcca ctcggcggcc gcgcgtacgt acggctgcgc catgtcttcg ccgatcgccg
      301 cgagcgccgg caacacggcg gccgttccgt agatgtgatt gacgccccag cggccgaacc
      361 agctgccgtc ggcttcctgc tcggagcgga tgaacttgaa cccgcgcgcg acggccggat
      421 ggtccagcgt gaagccgagc agcccgagcg cctcgagcac gtgcgccgtc acgtccgcgg
      481 acggcggatc gagcgcctcg ccgaaatcgc agaacggtat tttcgtgatg atgggccggt
      541 cgttgtcctt gtcgaacgcg gcccagccgc cgctgctcga ttgcatcgcg agcgtccagc
      601 cgagcgcgcg atcgatcgtc gcgcgaatcg gcggctcgtc gagcaggtgg cgcggtaagc
      661 gcgcgagcac gatcaacgcg acggccgtgt cgtcgatgtc cgggtagtgc aagttggcgc
      721 gctcgaaagc ccagccgctc ggctcgacgc ctttgacttt gcgcgtccag tcgccgtgat
      781 accgaacctc gttcgcgagc acccaatcga gcgcgcgagt catcgccggc gtgaactcgc
      841 gctcgcagtc ttgcagcgcg agcagcgcga gcagcgtgtc ccagaccggc gactcgctcg
      901 cctgaatgtg cagcgtgccg ccgcgctcgt acgaccaatg cgagtcgagc gccgcgaggc
      961 ctgccgtcat gaccggatgg tcgagcctgt agccctcgac gttgagcgcc atgagactgt
     1021 agatccacgg cggctggatg ccgccccacg cgccgtcggc gtcttgatgc cggacgatcc
     1081 aaccgaggca cgcgttgatg gccgtctcgc ggaacggcgt gaagccgagc tgttggtacc
     1141 agtgcagcac gcgatccgac aggaggaaca accgccgcca cgacagccac gggccgcgcc
     1201 gcggcaggct gtagtccatg cgctcgcggc cctccggaaa gagctcgtcg agccgccggc
     1261 cgggcggcag cggccgcacg gcgcgccgtg cggacagcac cgcgagcggc agcagcgtcg
     1321 cgcgggccca gctcgcgaag ttgtaaatgt tgaaagggaa ccagcgtggg ttcgcgatga
     1381 cctcgggcgg cagattcggc gtcgcgctcc acggccattc gccgagcagc gcgagccaat
     1441 agcgcgtgaa cacgcggatg ccgcgcaagc cgccgtgctc gaggatccac cgccgagcgc
     1501 gcacgagcgg ctcgtcggcg gggtcgaagc cgacgctgcg cagcgcggcg tagcactcga
     1561 cggttccgtt gatgtcgccc tgcggcgcgt cgtagtacgc ctcccacgag ccgtccggcc
     1621 gctgcgcgtt cagcagcgtc gcgacgaggt cctgctcgcg cgggtgcttg cgcccgagga
     1681 agtgcatggc gagcagccat tcggcctcca tgcaggagtt cgactcgagc atcccgaccc
     1741 agaagccttc gggcgtctga tcgcggtcga gccatgcgat cgctttggcg atcgtggcct
     1801 cgacctgctc ggcgggagta tcgagagcgc gaggcgcgga aacgagcata accaccgaca
     1861 gtgacggaaa gcgcgcgcat tatcgcacaa cgccttgagg cgcctcggat aggggccact
     1921 ccgcgctctt gcgctgcgcc gccccgaggc ccgagcatgc gggaacgccg ccattttcgc
     1981 cggaccgcca tggccctccc gcgctcagcc gccgtctgcc tgcttcttgc cttatcggga
     2041 tgtgcggccg tgccgacggc cggcggccag cgcctgaaac tccgctccgc cgagttccgc
     2101 gactacgtag agcgtgtgtt tcgcgagcag aacagggtcg ccgacgcgct cgctttcgcg
     2161 ctcgaagcgc cgggctcgcc ggatgccgag cttgccgccg ccgagcagga gctgctcgcg
     2221 gcctgcgcgg gcgtcaacga gctcgcgacg gcgcgccgcg atcagcggcg gcttgggctt
     2281 agaagcagcg tccgcgccgc gaagagcgtg cccgactgcg agcgcgcggc gcttgccgcc
     2341 gaggcacggt tgtctcgcaa agagtgagcg tcagtccggc aacagccggc cgactgcgac
     2401 gagatgctcg cgatagtacg gcgagtcgag gcttgcgacg gcgacgttct ggccgctcgc
     2461 cggcgcgtgc acgaacatgc cgtcgcccag atagattccc acgtgcgaca gcttcgtctg
     2521 atcctggaag aacatgaggt cgcccggatc ggcgtcgccg accgagatct tgcgcgcggc
     2581 gcggaacaac tcctgcgacg tgcgcggcac gcggtagccc gcttgcccgt acgcgtaata
     2641 gacgagaccg ctgcagtcga acccttcgag cggatccatg ccgccgaagc gatagcgagt
     2701 gccgaccatg cccatcgcga cgtcggcaat cgcgccgccg acgacttcgt cggcgactcg
     2761 cggctctacc gtcatgcgcc gcggcgcctc ggcggccggc gcgacgcggg ggcctgcaca
     2821 gcccgtcgtc gccgccgcga ccgcggcgag catcgtcagc atggctgtgc gtgggaaccg
     2881 aaacatgcgg cagagactat gcggccctag ctgaatcgac cctgaagacg aggcagaatt
     2941 ctacgcacgc gattcatcgc gtcgctgcag cgcatcacag aaatttggtc gtcgtacgac
     3001 gacggttccg agtcagcgtt tcgcgagcca agggggcaac ggcaggtcct tggccttcag
     3061 gaactcgggg ttgaacagct tcgattgata acgcgagccc gagtcggcga gcacggtcac
     3121 gattgttttt cccggaccga gctggcgcgc gagccgaatc gcgccggcga cgttgatgcc
     3181 gctcgagccg ccgagcagca gcccctcgtt ttgtaaaaga tcgtagagga caggcagcat
     3241 ctcggtgtct ggaatttgga atgcatcgtc gatgagggcg ccatcgaggt tgccggtcac
     3301 gcgcccctgg ccgatgcctt cgctgatcga gctgccttgc cccttcagct ctcccgtctt
     3361 gatccagctg tagatcgcgg cgcccatcgg atcggcggcg gccgtcacga tgttgcggtt
     3421 cttgtgcttc aagaaggcgc tgacgccggc cagcgtgccg cccgtgccga cggcgcagat
     3481 gaagccgtcg atcgagccgt tcgtttgctc ccagatctcg gggcccgtgc tcgccgtgtg
     3541 gccgtcgcga ttcgcagggt tgtcccattg attcgcatag aggaccccat gctgctcggt
     3601 cgcctcgagc tccttggccg tgcgcgcggc gatgtgcacg tagttgttcg ggtccttgta
     3661 cggaactgcc gggacctcgc gcagctccgc gccgcagagg cgcagcgtat ccttcttctc
     3721 ctgcgactgc gtgttcggca tcaggatcaa cgtcctaagc ccgcgcgagt tgccgacgag
     3781 cgcgagcccg atgccggtgt tgccggccgt gccctcgacg atgagaccg
//
LOCUS       DASSNN010000173         3459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_648530, whole genome shotgun sequence.
ACCESSION   DASSNN010000173 DASSNN010000000
VERSION     DASSNN010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648530"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..158
                     /locus_tag="VFL84_03080"
     CDS             3..158
                     /locus_tag="VFL84_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7607634.1"
                     /translation="MHPSLTTVRQDLARGASLLVDLVLRRIAGENTEPVLLPAELVVR
                     ESSGRGS"
     gene            complement(170..1507)
                     /locus_tag="VFL84_03085"
     CDS             complement(170..1507)
                     /locus_tag="VFL84_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HET7607635.1"
                     /translation="MKISPTFTLLAVVLGAPAWSSAEPRLRAGAAKVDVTPAERELPP
                     GYEGILDRIYSRAIVIGDGTSLAALISVDAGGVPDAIWAEVTRRLESELGIPTDNVLL
                     TATHTHSAPRQGAGPYTDKIVESARLAKARLVDARVGYGTGVSYLNVNRNIIDPKTRR
                     WWEGPNYDGPSDKTVAVIAFEDSRGEPIAVYYNYAMHAVTVGQLDMVSADAPGTASKY
                     IEDSFDDKVVALWSSGAAGDQNPIYYQQTYDLREIRVKDYAARGVDISNAMPPGGQGL
                     NKQDPTVMRLMNQQKQMIQSMGQFLGEEVLHVMRGMERMDSGAAIAGRHKIVSCPGRE
                     RTNEGRAGGPGEYREAGPVDIRLGLLELGDIRIGAVNAEVFNPIAQRLKRESPYARTM
                     LATLTNGTARSGYIPHDAGFGMYTFEVLSSRLQPGCAESAIVDGLLELMSPGG"
     gene            complement(1518..2696)
                     /locus_tag="VFL84_03090"
     CDS             complement(1518..2696)
                     /locus_tag="VFL84_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7607636.1"
                     /translation="MMFFTFAMTTDAVGSIIPTLLTEFNLSLRAASAFHYAPMIAIAV
                     GALGLGFLADRIGRKLTIVIGLALYGLSSLLFAFGSGFGLFVVLLAAGGIGISVFKIG
                     ALALIGDVARSTREHTTFMNTVEGFFAVGAIVGPAIVATLLAAGLSWKYLYVIAALIC
                     AALLAAAALARYPSTVRAEQGRGSFADTLRKMRDAHALGFSALIMLYVAVEVAVYVWM
                     PTYLQGYTGAASWLATYALTIFFVLRAAGRFLGAWFLRKLSWSTVLVTFAAAIVVCFA
                     GSLYAGPDLAVYLLPLSGLFMSVLYPTLNSKGISCFPRSEHGAVAGVILFFTAFAAAV
                     GPLAMGAISDALGDIKYGFVLATGFAALLFAGLLINRVFDPAGARLARRDDSDYATAH
                     "
     gene            2873..>3459
                     /locus_tag="VFL84_03095"
     CDS             2873..>3459
                     /locus_tag="VFL84_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET7607637.1"
                     /translation="MKNAVQLITYVDRLSGGGFAELIALLRGPLRGLFGGIHVLPFFD
                     PIDGADAGFDPRDHTRVDARLGDWSDVAALAAEVDVMADLIVNHTSRDSPQFRDFLTQ
                     GAASPFAELFLTRERVFANGATDAELRAIYRPRPGLPFTETTTGDGTKRHMWTTFTPS
                     QVDIDLTHARGREYVDAILAKLAASGVRIVRLDAVG"
BASE COUNT          609 a         1158 c         1131 g          561 t
ORIGIN      
        1 acgtgcaccc gtcgttgacg acggttcggc aggatctcgc gcgcggcgcg agtctcctcg
       61 tcgacctcgt gctgcggcga attgcgggcg agaacacgga accggtccta ctacccgccg
      121 agctcgtcgt gcgcgaatcg agcggccgtg gctcgtgacg gcactcgagt cagccgcctg
      181 gcgacatcag ctcgagcagc ccgtcgacga tcgcggattc ggcgcacccc ggttgcaacc
      241 gtgaagacag gacttcgaac gtgtacatgc cgaatccggc gtcgtgcggg atgtagcccg
      301 agcgcgcggt gccgttcgtc agggtcgcca gcatcgtgcg cgcatacggc gactcgcgct
      361 tcaagcgctg tgcgatcggg ttgaacacct cggcgttaac ggcgccgatc ctgatgtcgc
      421 cgagctcgag cagcccgagt cgaatgtcga ccggcccggc ctcgcggtat tcgcccgggc
      481 cgccggcgcg gccttcgttc gtccgctcgc ggcccgggca gctcacgatc ttgtggcggc
      541 ccgcgatcgc ggcgcccgag tccatccgct ccatgccgcg catcacgtgc agcacctctt
      601 cgcccaagaa ctgacccatc gattgaatca tttgcttctg ctggttcatc agccgcatta
      661 ccgtcggatc ttgcttgttg agcccctgcc cgcccggcgg catcgcgttg ctgatgtcca
      721 cgcctcgcgc cgcgtagtcc ttgacgcgta tctcgcgcag atcgtaggtc tgctggtagt
      781 agatcgggtt ctgatcgccg gccgcgccgc tcgaccagag cgcgacgact ttgtcatcga
      841 acgaatcctc gatgtacttc gaggcggtgc cgggcgcatc ggcgctgacc atgtcgagct
      901 gcccgacggt caccgcgtgc atcgcgtagt tgtaatagac ggcgatcggc tcgccgcgcg
      961 aatcttcgaa cgcgatgacg gccaccgtct tgtccgacgg cccgtcgtag ttcggacctt
     1021 cccaccagcg ccgcgtcttc ggatcgatga tgttgcgatt gacattgagg taggacacgc
     1081 ccgtgccgta cccgacgcga gcgtcgacga ggcgagcctt cgcaagccgc gccgactcca
     1141 cgatcttgtc cgtgtagggc ccggctccct gccgcggtgc gctgtgcgtg tgcgtcgcgg
     1201 tcaggaggac gttgtcggtc ggaataccga gctcgctctc gagccgccgc gtcacttcgg
     1261 cccagatcgc atccggcact ccaccggcat cgacgctgat cagcgccgcc agcgacgtgc
     1321 cgtcgccgat cacgatcgcg cgcgaataga tacggtcgag tattccctcg tagcccggcg
     1381 gcagctcgcg ctcggcgggc gtgacgtcga ctttcgcggc gccggctcgc agccgcggct
     1441 cggccgacga ccaggcgggt gcgccgagca cgacggcgag cagcgtgaac gtcggcgaaa
     1501 tcttcatgcg tctctcctca gtgcgccgta gcgtaatcgg agtcatccct tctcgcgagg
     1561 cgcgcgccgg cgggatcgaa cactcggttg atcagtaagc ccgcgaacag cagcgcggcg
     1621 aaccccgtcg cgagcacgaa gccgtacttg atgtcgccga gcgcgtcgct gatggcgccc
     1681 atcgcgagcg gcccaacggc ggcggcgaag gccgtgaaga acaagatcac gcccgcgacg
     1741 gcgccatgct cggagcgtgg aaaacaactg atgcctttcg agttcagagt cgggtagagc
     1801 accgacatga agagacccga caacggcagc aggtagacgg ccaggtccgg gccggcgtac
     1861 aggctccccg cgaaacagac gacgatcgcc gcagcgaacg tcacgagcac cgtgctccac
     1921 gacagcttgc gcaggaacca agcgccgagg aagcggcccg cggcgcgcag cacgaagaag
     1981 atcgtcaggg cgtaggtagc gagccaagac gcggcacccg tatagccctg caggtaagtc
     2041 ggcatccaga cgtagacggc cacctcgacg gcgacgtaca gcatgatcag cgcggagaag
     2101 ccgagcgcgt gcgcgtcgcg catcttgcgg agcgtgtcgg cgaacgagcc gcggccctgc
     2161 tctgcccgca cagtggacgg gtagcgcgcc aaggcggcag cggccaggag ggctgcgcag
     2221 atcaaagccg cgatgacgta aaggtatttc cacgacaggc ccgcggccag caacgtcgcg
     2281 acgatcgccg gcccgacaat ggcgccgacg gcgaaaaatc cctcgacggt gttcatgaac
     2341 gtcgtgtgct cgcgcgtcga gcgcgcgacg tcgccgatca gcgccagcgc gccaatcttg
     2401 aagacgctga tgccgattcc gccggcagcg agcaaaacga cgaacaggcc gaatccgctg
     2461 ccgaaggcaa acagcagcga gctgagcccg tacagtgcga gaccgatgac gatcgtgagc
     2521 ttgcggccga tgcggtctgc gaggaaaccc aagccgagcg cgccgacggc gattgcgatc
     2581 atcggcgcgt agtgaaaagc actggccgcg cgcaacgaca gattgaactc cgtgagcagc
     2641 gtggggatga tgctgccgac ggcgtccgtc gtcatcgcga acgtgaagaa catcatgcac
     2701 gtgagcgcct tgatcaaaag cagttcaggg cggctgcgtg cgcgctccgg gctctccatg
     2761 gaccccccga tccggaagct cgacgccacg tgatcggcgt cgaacctggc tttacgcgtt
     2821 ttgcaatcga ttgcatgata gccaccgctc gccgcctgcg taaacggtcg cgatgaagaa
     2881 tgccgtccag ctgatcacgt acgtggaccg cctgtcgggc ggcggttttg ccgagctcat
     2941 tgcgctgctg cgcgggccgc tccgcggcct cttcggcggc attcacgtgc tgccgttctt
     3001 cgacccgatc gatggggctg atgcaggctt cgatccgcgc gaccacacgc gcgtcgacgc
     3061 tcgcctcggt gactggagcg acgtcgcagc gctagcggcc gaagtggacg tgatggccga
     3121 cctcatcgtc aaccacacct cgcgcgactc gccgcagttt cgcgacttcc tcacgcaggg
     3181 agcggcgtcg ccgtttgcgg agctgttcct gacgcgcgaa cgcgtgttcg caaacggcgc
     3241 aaccgacgcc gagctccgtg caatctatcg gccgcgtccc ggtctgccgt tcaccgaaac
     3301 gacgactggt gacggcacca agcggcatat gtggacgacc tttactccaa gccaagtcga
     3361 tatcgacctc actcacgccc gtgggcggga gtacgtcgac gcgattctcg caaagctcgc
     3421 cgcgagcggc gtgcggatcg tgcggctcga tgcggtcgg
//
LOCUS       DASSNN010000174         3889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1279640, whole genome shotgun sequence.
ACCESSION   DASSNN010000174 DASSNN010000000
VERSION     DASSNN010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279640"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFL84_03100"
     CDS             complement(<1..631)
                     /locus_tag="VFL84_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607638.1"
                     /translation="MQLEALALRMRPRAPLEAADLGVRLCQDAARSVYACYAVAYIPI
                     AALALASFEIASWLPTLFLFCAKPWLDRTILFVLSRAAFGQPTTVRQLWDARGQVWGR
                     QLLLTWTWRRLSPWRSFTQPVYQLEGLPFGKIRRRVKQVRRRKRGAALLMMAAFATAE
                     TCLGFTLLSLLVWFAPTEAAGDLLEVMFDPTSELFEASMAVAYAVVIAFL"
     gene            complement(621..1598)
                     /locus_tag="VFL84_03105"
     CDS             complement(621..1598)
                     /locus_tag="VFL84_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HET7607639.1"
                     /translation="MTPLAFEALYQAEWQELEEQLDRLLRRTSNRSQEPPRGERIAAL
                     YRRACEHLALARARSYPAYLLDRLDRLTADAHQVIYQQREFGGSALWRVIARDFPRAV
                     RKESAYVWLATVLFTAPLLIVGVLVYYQPSLILSVVDAETAAQFEQMYSGSGEAIGRA
                     RDAGDDWAMYGFYVRNNISVAFQCFASGIAVGLGSIFYLMFNGASIGGVAGYLTERGL
                     GETFWSFVATHSAFELTAIVISGAAGLKLGHSLLAPGRRTRHQSLVQAARECVVIVYG
                     VAAMLLVAAAIEAFWSSATWVPSVVKYSVAGVCWLVVVAYLTLQGRHAA"
     gene            complement(1595..2299)
                     /locus_tag="VFL84_03110"
     CDS             complement(1595..2299)
                     /locus_tag="VFL84_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET7607640.1"
                     /translation="MARDVLDTVVAAETPEGIVLELRPAGLSARFYAFLLDWLVRLMI
                     AYAAAIASAFMGGLGLGFWLLFYFLLEWFYPVVFELSRSGATPGKSIVGIKVVMDNGL
                     PVTPAASFTRNLLRVADFLPFLFGAGVVSMLVRHDSKRLGDLAAATLVVHQPQRPPRI
                     KLDDVAPVTPTMPLAPSDQAALVALAARAPTLTGERLDELAALAAAVSGDGGRAGPNV
                     TRRVLGVAQWAMGKRA"
     gene            complement(2311..2823)
                     /locus_tag="VFL84_03115"
     CDS             complement(2311..2823)
                     /locus_tag="VFL84_03115"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET7607641.1"
                     /translation="MSTSNPYAPPRGTVRDMTAEGGELAGRGARLIAAILDSIIVGLM
                     VYVPALVVMAATGGLAQLDRPESEIDPSVLTLPIVVGCIGAIAWAWITLLLVARHGQT
                     MAKRLLEIKVVRSDGSKASLGRIFWLRNVVNGLLGVIPLYGLVDILFIFGERRQCIHD
                     LIADTIVVKA"
     gene            complement(2893..3378)
                     /locus_tag="VFL84_03120"
     CDS             complement(2893..3378)
                     /locus_tag="VFL84_03120"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HET7607642.1"
                     /translation="METLLVWLEGSALGELMRASGVWAYGVVNLVHILGIATLFGSVL
                     AFDLRLIGLWRRVPLAVLERPTLTLAACGFTVAVLSGIALLSTNGSEYAGNPFLELKF
                     TAIALGLTNIVAAQFLPAWRARAAEPHGPRQRFTLRVLGATSLVCWLGAVAAGRMIGY
                     W"
     gene            complement(3456..3833)
                     /locus_tag="VFL84_03125"
     CDS             complement(3456..3833)
                     /locus_tag="VFL84_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607643.1"
                     /translation="MRTRWLVGLTGAALGLLGVQASAHHGWAGQQQEQSEITGKLKTD
                     VNLSGPHGSMQIVDDKGQVWDVTLAPAARTQNAGLKPGVIPVGATVTIRGNRNSDPKR
                     FEMKTVRVTHDGKNYDVYPDRIS"
BASE COUNT          728 a         1337 c         1265 g          559 t
ORIGIN      
        1 cgagaaacgc gatgacgacc gcatacgcga cggccatcga agcttcgaac agctcgctcg
       61 tcgggtcgaa catcacctcg agcaggtctc cggccgcttc agttggcgcg aaccaaacga
      121 gcagcgacag cagcgtgaag ccgaggcacg tttcggccgt cgcgaacgcg gccatcatga
      181 gcagcgcggc gccgcgcttg cgccggcgca cttgcttcac gcgccgccga atcttgccga
      241 acggcagccc ttcgagctga tagacgggct gcgtgaacga gcgccacggc gacagccgcc
      301 gccaagtcca cgtcaacagc agctgacgcc cccaaacttg gccgcgcgcg tcccagagct
      361 gccgcacggt cgtcggctgc ccgaacgcgg cgcgcgacaa cacgaacaat atcgtccggt
      421 cgagccaggg cttcgcgcag aacaggaaca gcgtcggcaa ccagctcgcg atctcgaacg
      481 aggcgagcgc gagcgcggcg atcggaatgt aagcgacggc gtagcacgcg tacaccgagc
      541 gcgcggcgtc ttggcagagg cgcacgccga gatcggccgc ctcgagcggc gcgcgcggcc
      601 gcatgcgcag cgcgagcgct tcaagctgca tggcggccct gcagcgtgag atacgcgacg
      661 acgacgagcc agcaaacgcc tgcgacgctg tacttgacga cgctcgggac ccaagtagcc
      721 gaggaccaga acgcttcgat cgccgccgct acgaggagca tcgccgcgac gccgtagaca
      781 atgacgacgc actcgcgcgc ggcttgcacg aggctctggt gtcgcgtccg ccgtcccggc
      841 gcgagcagcg aatgtccgag cttgagcccc gcggcccccg agatcacgat cgccgtgagc
      901 tcgaatgcgc tgtgtgtcgc gacgaatgac cagaatgtct cgcccaatcc acgctcggtc
      961 aggtaaccgg cgacgccgcc gatgctggcg ccgttgaaca tcagatagaa gatgcttccc
     1021 aacccgaccg cgattccact cgcgaaacac tggaatgcga cgctgatgtt gttgcgaacg
     1081 tagaagccgt acatggccca gtcatcgccc gcgtcgcgcg cgcggccgat cgcctccccg
     1141 gacccggagt acatctgctc gaactgggcc gcggtctcgg cgtcgacgac cgagaggatc
     1201 aagcttggct ggtagtagac gagcacgccg acgatcagca gcggagccgt gaacagaacc
     1261 gtcgccaacc agacatacgc gctttccttg cgcacggcgc gcggaaaatc ccgagctatc
     1321 acgcgccaca gcgccgagcc gccgaactcg cgctgttgat agatgacctg gtgcgcgtcg
     1381 gccgtcaggc ggtcgaggcg atcgagcaag tacgccggat acgaccgcgc tcgcgcgagc
     1441 gcgaggtgct cgcacgcgcg ccgatagagc gccgcgatgc gctcgccgcg cggcggctcc
     1501 tgcgaccgat tgctcgtgcg gcgcagcagc cgatcgagct gctcctcgag ctcctgccat
     1561 tcggcttgat acagcgcttc gaacgcgagc ggcgtcatgc gcgcttaccc atcgcccact
     1621 gcgcgactcc gaggacccgc cgcgtgacgt tcgggcctgc cctgccgccg tcgcccgaca
     1681 cggcggcggc gagcgcggca agctcgtcga gccgctcgcc ggtcagggtc ggcgcacgcg
     1741 cggcgagcgc cacgagcgcc gcttgatcgc tcggcgccag cggcatcgtc ggcgtcacgg
     1801 gcgcgacgtc gtcgagcttg atccgcggcg gccgctgcgg ctggtgcacg acgagcgtcg
     1861 cggcggcgag atcacccagg cgcttgctat cgtgccgcac caacatcgag acgacgcccg
     1921 cgccgaacag aaacggcagg aagtccgcga cgcgcagcag gttgcgcgtg aacgacgcgg
     1981 ccggcgtgac cggcaagccg ttgtccatga cgaccttgat tccgacgatg ctcttgccgg
     2041 gcgtcgcgcc cgagcggctg agctcgaaga cgacgggata gaaccactcg agcaggaaat
     2101 agaagagcaa ccagaagccg aggccgaggc cgcccatgaa agcggatgcg atcgcggccg
     2161 cgtaagcgat catgagacga accagccaat cgagcaggaa cgcgtagaac ctcgcgctca
     2221 agcccgcagg acgcagctcg agcacgatgc cctcgggcgt ctcggcagcc acgacggtgt
     2281 cgagaacgtc gcgcgccatt cgtaagccgc ttacgctttg acgacgatcg tgtcggcgat
     2341 caggtcatgg atgcattggc gccgctcgcc gaagatgaac aggatgtcga cgaggccgta
     2401 gagcggaatc acaccgagca agccgttcac gacgttgcgc agccagaaga tccggccgag
     2461 tgatgccttc gagccgtcgc ttcgaaccac tttgatttcc agcaaccgct tcgccatcgt
     2521 ctggccgtgg cgcgcgacga gcagcaacgt gatccaggcc caggcgatcg ccccgatgca
     2581 gcccaccacg atcggcagcg tcagcacgct cggatcgatc tcgctctccg gccggtcgag
     2641 ctgcgcgagc ccgccggttg ccgccatcac taccaaggcc ggcacataga ccatgagacc
     2701 cacaatgatg ctgtccaaaa tggcggcgat gaggcgcgcg ccgcgcccgg ccagctcccc
     2761 gccttctgcc gtcatgtcgc ggaccgtgcc tcgcggcggt gcgtatggat tgctagtgct
     2821 catatcagcc cctcccggct gtgaaaacgc cgcgattctg acgtaaacca ggtggcgttg
     2881 tcgcgtgcag ggttaccagt agccgatcat gcggcccgcc gcgacggcgc cgagccagca
     2941 gacgagcgac gtcgcgccga gcacgcgcag cgtgaagcgc tggcgcggac cgtgcggctc
     3001 ggcggcgcgg gcgcgccatg ccggcaggaa ctgcgcggcc acgatgttcg tgagcccgag
     3061 cgcaatcgcc gtgaacttca gctcgaggaa cggattgccg gcgtactcgc tgccattcgt
     3121 cgagagcagc gcgatgccgc tcaagacggc caccgtgaag ccgcaggccg cgagcgtcag
     3181 cgtgggccgc tcgagcacgg cgagcggcac ccggcgccac agcccgatca gccgcaagtc
     3241 gaacgccagc accgacccga acagcgtcgc gatgcccaag atgtgaacga gattgacgac
     3301 gccatacgcc catacgccgg atgcgcgcat gagctccccg agggcggaac cctcgagcca
     3361 gacgagcagc gtttccaagc gcgagccgcg ggacggatct attttcgagg accgcgggac
     3421 ggatctattt ttcgacgaaa atagatctgt cccctttagc taattcggtc cggatagacg
     3481 tcgtagttct tgccgtcgtg cgtgacgcgg accgtcttca tctcgaaacg cttcggatcg
     3541 ctgttgcgat tgccgcggat cgtgaccgtc gcgccaaccg ggatcacgcc gggcttcaag
     3601 ccggcgttct gcgtgcgcgc ggccggcgcc aacgtgacgt cccaaacctg gcccttgtcg
     3661 tcgacgatct gcatcgagcc gtgcggcccg gagaggttca catccgtctt gagcttaccc
     3721 gtgatctcgc tctgctcttg ctgctggccc gcccatccgt ggtgcgccga agcttgaacg
     3781 ccaaggagtc ccagggcggc gccggtcaac ccaaccaacc aacgtgtgcg catgactgcc
     3841 tcctcaatag ctcgtcatgg gatggtacgc ctaacgggcg tcgaggcgc
//
LOCUS       DASSNN010000175         5259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1783119, whole genome shotgun sequence.
ACCESSION   DASSNN010000175 DASSNN010000000
VERSION     DASSNN010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783119"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFL84_03130"
     CDS             <1..398
                     /locus_tag="VFL84_03130"
                     /inference="COORDINATES: protein motif:HMM:NF014418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET7607644.1"
                     /translation="LLEDKRSSLAVHFRERPELAATVQRRLEEAAALVGEGFRIQESV
                     LVREIVAAAAHKGDAVKAFLAEPAFAGRMPVFVGDDVTDLDAFAAVERHGGRAVAVGP
                     RLPASWRLPSPSAVLDWLELLADSDGRWM"
     gene            481..1200
                     /locus_tag="VFL84_03135"
     CDS             481..1200
                     /locus_tag="VFL84_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607645.1"
                     /translation="MRLIPLTAVTLVVAMPLSAQDWQTTTVTGDGFRANFPGEPTVEP
                     ITYETEFRLHLPGHVYRAADALGKYSTTVVDYRDVQRLHDERSAKCLAAKGANQQDGD
                     TCQNDFVMEVAGAMDHAAAAFLQRDGVKVTHYGTYFLDMVSGRLIQLTNPDRSRTYVA
                     IHQHAGRLYIHEATVPPAMPEPILFEQGLAWVDADGKAIRYRTLYTEGYADWKFPQPV
                     PPPHTLRDLDASREQPPRPPR"
     gene            1211..1603
                     /locus_tag="VFL84_03140"
     CDS             1211..1603
                     /locus_tag="VFL84_03140"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7607646.1"
                     /translation="MLRKLIAAALPALLGASAPALAHHSHANYDITKWVVVEGTVKQV
                     VFIAPHSLVYLDVKNDKGETSVWALEATAPAGIFGNGVGRQDVRAGDLIKARCHQLRD
                     GADGCLLGFITPLHGDVARGNGIEREWD"
     gene            complement(1626..2549)
                     /locus_tag="VFL84_03145"
     CDS             complement(1626..2549)
                     /locus_tag="VFL84_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7607647.1"
                     /translation="MKRSLHEVGEALVRRLVLVCAAALVLAACAPETSDAIGGETQWI
                     DVAGGKLKTQVFEHVGDGRRPILVLVLHGDLPNPPPDYQYLFAKLLVAGISEATGRSA
                     ALRAALGPRWERKSIVAAGILRPGYADPSGDRSSGEMGKAVADNYTPEVVDAVAAAAR
                     ELKAKYDASAVVLVGHSGGGAIAANVLGRHPDVASYALLVACGCDPEAWRARMRARQP
                     DPVWDEPISSLMPLSLVDGIARDTRVRLLAGENDDVVLPADIARYAVALRARDIEVRL
                     TVAPGLGHNILMSPEAFRELSLLVGALGDAE"
     gene            2645..3604
                     /locus_tag="VFL84_03150"
     CDS             2645..3604
                     /locus_tag="VFL84_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7607648.1"
                     /translation="MKSYWIVQSPRGLELEPRDIPTPKPNEGQLLVKVRASSFNRGEL
                     LTGHGAFDPNGKQAGIECAGEIASDAAGFRAGERVMGRCVGGFAEYALMDAREAMRIP
                     NRRSWEQAAAIPLAFMVAHDMLVAHGRLQRGQWLLITGVSSGVGVASLLVGKALGANV
                     IGTSGSEEKLAQLAKLGLDVGVATRGNGFHEPAMKATAGKGVNLVVNTVGGTVFAECV
                     RTLAFEGRLATVGYLDRTLKAEIDLEALHAKRLHLFGVSNKLRPAAERAQTVRAFEKD
                     LLPYFADGRIAPIVDRVFAFDDLAAARAYFDSGAFVGKVVVRM"
     gene            complement(3615..3929)
                     /locus_tag="VFL84_03155"
     CDS             complement(3615..3929)
                     /locus_tag="VFL84_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET7607649.1"
                     /translation="MTRGYTSIWAFEVHPEHVVAFRWHYGEGGAWTQLFRRARGYLGT
                     RLLEDRNDPLRFVTIDIWSSVEDYEAFRAEHASEYAALDRVCEGFTVTETLIGHFVSA
                     AQ"
     gene            complement(3945..4655)
                     /locus_tag="VFL84_03160"
     CDS             complement(3945..4655)
                     /locus_tag="VFL84_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET7607650.1"
                     /translation="MALVEISRSLSERVASLRFADPVCCVYNPLDYARAPHELYLSRY
                     GNGTKEVLLLGMNPGPFGMVQTGVPFGDVAMVRDWLGIVGPVGKPRDEHPKRPVLGFE
                     CHRSEVSGARLWGFARDRFGTPQRFFERFFVANYCPLAFMESGGANRTPDKLLAAEQK
                     ALCGACDDALREVVRELRPRLVVGVGGFAERRARAALAGQNVAIGTILHPSPASPLAN
                     QGWAEKIEQQLRGMGIDV"
     gene            complement(4656..>5259)
                     /locus_tag="VFL84_03165"
     CDS             complement(4656..>5259)
                     /locus_tag="VFL84_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007301222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HET7607651.1"
                     /translation="VPATPGRTVFQTGAWVLADRTPLPAHLEAFLDSGDSPVFVGLGS
                     MPAAEDVSRPLIGAARAVGRRILVSRGWAGLDVIDEAGDCLAIGDVSHELLFPRVACV
                     VHHGGAGTTAAAARAGVPQVVAPMFGDQPYWAGRVVELALGATVPYSAITEESLANAL
                     RRALDPAAAARARAFAAQVRADGAAVAVRRLEAQLGTATA"
BASE COUNT          857 a         1845 c         1758 g          799 t
ORIGIN      
        1 tgctgctcga ggacaagcgc agctcgctgg ccgtgcattt tcgcgagcgg ccggagctcg
       61 ccgctaccgt tcagcggcgg ctcgaagaag ccgccgcgct cgtcggcgag ggattccgca
      121 tccaggagag cgtgctcgta cgcgaaatcg tcgcggccgc cgcccacaaa ggcgacgccg
      181 tgaaagcctt tctggcggaa ccggcattcg ccggccgcat gccggttttc gtcggcgacg
      241 acgtgacgga cctcgacgcg tttgccgcgg tcgagcgcca cggcggccgt gccgtcgcgg
      301 tcggcccgcg cttgccggcc tcgtggcgcc tcccttcgcc atcggcggtg ctcgattggc
      361 tcgagctctt ggcggactcg gacggccgct ggatgtaacg cggcgccgcg ccactgttgc
      421 cttcgctgcg cgccctcggc aacatcatcg tgacccgacg cggtggaaac gggggaagcc
      481 atgcgcttga ttccactgac cgcggtaacg ctcgtcgtcg ccatgccgct ctctgcccag
      541 gactggcaga cgacgacggt cacgggggac ggcttccgcg cgaacttccc cggcgagccg
      601 accgtcgagc cgatcaccta cgagaccgag ttccggttgc acttgccggg tcacgtctat
      661 cgcgcggccg acgcgctcgg taagtactcg acgaccgtcg tcgactatcg cgacgtgcag
      721 cgcttgcacg acgagcgctc ggcgaaatgt ctcgccgcca agggcgcgaa ccaacaggac
      781 ggcgacacgt gccagaacga ctttgtcatg gaggtcgcgg gcgcgatgga ccacgccgcg
      841 gcggcgttct tgcaacgcga cggcgtgaag gtcacgcact acggcaccta cttcctcgac
      901 atggtgtcgg gccggctcat ccagctcacg aacccggacc gctcgcgcac ctacgtcgcg
      961 atccatcagc acgcgggccg gctctacatt cacgaggcca cggtgccgcc ggcgatgccg
     1021 gagccgatcc tgttcgagca agggctcgcg tgggtcgacg ccgacggcaa ggccattcgc
     1081 tatcgaacgc tgtataccga gggctacgcc gactggaaat tcccgcagcc cgtgccgcct
     1141 ccgcacacgc tgcgcgacct cgacgcgtcg cgcgagcaac cgcctcgccc gccacgttag
     1201 gagaagggcc gtgttgcgaa aactaattgc tgccgctctg cccgcgttgc tcggcgcgag
     1261 cgcgccggcg ctcgcgcacc actcgcacgc gaactacgac atcacgaagt gggtcgtcgt
     1321 cgaaggaacg gtcaagcaag tcgtcttcat cgcgccgcac tcgctcgtct acctcgacgt
     1381 caagaacgac aagggcgaga cgtcggtttg ggcgctcgag gccacggcgc cggccggtat
     1441 cttcggcaac ggagtcggcc gccaggacgt gcgcgccggc gacctgatca aggcgcgctg
     1501 ccatcagctc agggacggcg ccgacggctg cttgctcggc ttcatcacgc cgctgcacgg
     1561 cgacgtcgcg cgcggcaacg gcatcgagcg cgagtgggac tgatccgctg gctttcgccg
     1621 cacgctcact cggcgtcgcc gagcgctccg accaagagac tcaattcgcg aaacgcttcc
     1681 ggcgacatca agatgttgtg accgagcccc ggcgcgaccg tcagccgcac ttcgatgtcg
     1741 cgcgcccgca gcgcgacggc ataccgcgca atgtccgccg gcaagacgac gtcgtcgttt
     1801 tcgccggcca agagacgcac tcgcgtgtcg cgagcgatcc cgtccacgag cgacaagggc
     1861 atgagactcg agatgggctc gtcccatacc ggatccggtt gccgcgcacg catccgcgcg
     1921 cgccacgctt ccgggtcgca cccgcaagcc acgagcaagg cgtagcttgc gacgtccgga
     1981 tgtcgaccga gcacgttcgc ggcgatggca ccgccaccgg agtggccgac gaggacgacc
     2041 gcactcgcgt cgtatttcgc cttgagctcg cgtgccgcgg ccgcaacggc gtcgacgacc
     2101 tcgggcgtgt agttgtcggc gaccgccttg cccatctcgc ccgacgaccg atcgccggac
     2161 gggtccgcgt agccgggacg aagaatgccg gccgcgacga tactcttccg ctcccaacgt
     2221 ggaccgagcg cggcgcgcaa tgcggcagac cgccccgttg cttccgaaat ccctgctacg
     2281 agcagcttcg cgaagaggta ttgatagtcg ggcggtgggt tcgggaggtc gccgtgcagg
     2341 acgagaacga ggatgggccg gcggccgtca ccgacgtgct cgaatacctg cgttttcagc
     2401 ttgccgcccg caacgtcgat ccactgcgtt tccccgccga tcgcgtctga agtctcgggg
     2461 gcgcacgcgg cgaggaccag tgccgcggca cagacgagca cgaggcgccg aacgagcgct
     2521 tcgccaacct cgtgcaatga gcgcttcaac tgctgcaccc gttcccccgc tgtcacggcg
     2581 cgcgttgcga accctcgacg tgccgtatta tcggcattcg ccttgcggag gctagagcgt
     2641 cgcgatgaaa tcgtattgga tcgtgcaatc gccgcgcggg ctcgagctcg agccgcgcga
     2701 catcccgaca ccgaagccca acgaaggcca gctgctcgtc aaggtgcgag cttcgtcgtt
     2761 caatcgcggc gagcttttga ccggccacgg cgctttcgac ccgaacggca agcaagcggg
     2821 catcgagtgc gccggcgaga tcgcgagcga cgcggccggt ttccgcgcgg gcgagcgcgt
     2881 gatgggacgc tgcgtcggcg gtttcgccga gtacgctttg atggatgcgc gcgaagcgat
     2941 gcgcatcccg aatcgccggt cgtgggagca agcggccgcg attccgctcg cgttcatggt
     3001 cgcgcacgac atgctcgtcg ctcacggccg gctccagcgc ggccaatggt tgttgatcac
     3061 gggcgtgtcg tcgggcgtcg gcgtcgcgtc gttgctcgtc ggcaaggcgc tcggcgccaa
     3121 tgtcatcgga acctcgggct ccgaggagaa gctagcccag ctcgcgaagc tcgggctcga
     3181 cgtcggcgtc gcgacacgcg gcaacggttt tcacgagccg gcgatgaaag cgaccgcggg
     3241 taaaggagtg aacctcgtcg tcaacacggt cggcggcacc gtcttcgccg agtgcgtgcg
     3301 cacgctcgcg ttcgagggca ggctcgcgac cgtcggctac ctcgatcgca cgctgaaggc
     3361 cgagatcgac ctcgaagcgc tgcacgcgaa gcgcctgcat ctcttcggcg tcagcaacaa
     3421 gctgcgcccg gccgccgagc gcgcgcaaac ggtgcgcgcg ttcgagaagg acttgctccc
     3481 ctacttcgcc gacggccgca tcgcacccat cgtcgatcgc gtgttcgcgt tcgacgactt
     3541 ggccgccgcg cgcgcgtact tcgactcggg cgcgttcgtc ggcaaagtcg tcgtccgcat
     3601 gtgacgcacg ggcgctactg cgccgcgctg acgaaatgtc cgatcagcgt ctcagtgacg
     3661 gtaaagcctt cgcaaacgcg gtcgagcgcc gcgtactcgg aagcgtgctc ggcgcgaaac
     3721 gcttcgtagt cttcgacgct gctccaaatg tcgatagtta cgaaacgcag cggatcgttc
     3781 cggtcctcga gcagccgagt gcctagatag ccgcgcgcgc gccgaaacag ctgtgtccac
     3841 gcgcctcctt cgccgtagtg ccaacgaaag gccacgacgt gctcgggatg aacctcgaat
     3901 gcccagatgg atgtgtagcc tcgcgtcata ggagctccgc cgtttcacac gtcgatgccc
     3961 attccccgta gctgctgctc gatcttttcc gcccagcctt gattcgcgag cgggctcgcg
     4021 gggctcgggt gaagaatcgt tccgatcgcg acattctgcc cggcgagcgc ggcgcgcgct
     4081 cgccgctcgg cgaagccgcc gacgccgacg acgaggcgcg gccgcagctc tcgcacgacc
     4141 tcgcgcagcg cgtcgtcgca ggcgccgcac agcgccttct gctcggctgc gagcagcttg
     4201 tccggcgtgc gattcgcgcc gcccgactcc atgaacgcga gcgggcaata gttcgcgacg
     4261 aagaaccgct cgaagaagcg ctgcggcgtg ccgaagcgat cgcgtgcgaa accccagagc
     4321 cgcgcgccgc tgacctcgga gcggtgacac tcgaagccga gcacgggccg cttcgggtgc
     4381 tcgtcgcgcg gtttgcccac cggaccgacg atgccgagcc agtcgcgcac catcgcgacg
     4441 tcgccgaacg gcacgcccgt ctgcaccatg ccgaagggcc ccgggttcat gccgagcagc
     4501 agcacttcct tcgtgccgtt gccatagcgg ctgagataga gctcgtgcgg cgcgcgcgca
     4561 tagtcgagcg ggttgtagac gcagcagacc ggatccgcga agcgcaggct cgcgacgcgc
     4621 tccgacaacg agcggctgat ctcgacgagc gccatctacg cggtcgccgt tccgagctgc
     4681 gcctcgagcc gacgcacggc gaccgctgcg ccgtcggcgc ggacttgtgc cgcgaacgcg
     4741 cgcgctcgag ccgccgccgc gggatccaac gcccgccgaa gcgcgttcgc gagcgattcc
     4801 tccgtgatcg ctgagtacgg aacggtcgcg ccgagcgcga gctcgacgac tcgtccggcc
     4861 cagtagggct gatcgccgaa catcggcgcg acgacttgcg gaacgccggc gcgcgcggcg
     4921 gccgccgtcg tgccggcgcc gccgtgatgc acgacgcacg cgacgcgagg gaacagcagc
     4981 tcgtggctca cgtcgccgat cgcgagacag tcgccggcct cgtcgatgac gtcgagaccc
     5041 gcccagcctc gcgacacgag aatgcggcgg ccgacggcgc gcgcggcgcc gatcagcggg
     5101 cggctcacgt cctcggccgc cggcatgctg ccgaggccca cgaagacggg cgaatcgccg
     5161 ctgtccaaga acgcttcgag atgcgcggga agcggcgtgc gatcggcgag cacccaagcg
     5221 cccgtctgaa agaccgtccg gcccggggtc gcaggcacg
//
LOCUS       DASSNN010000176         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_901655, whole genome shotgun sequence.
ACCESSION   DASSNN010000176 DASSNN010000000
VERSION     DASSNN010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901655"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="VFL84_03170"
     CDS             <1..1304
                     /locus_tag="VFL84_03170"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7607652.1"
                     /translation="PSNTKNIGASADETADTVPLMSFERRERERTVVDHTLTGVRPIR
                     QAILDLSADVRQRADAAEKARQVPAETMASLRKAGLFRAFVPRVYGGDERSLTEVLDA
                     MTELAAACPSTAWVGTLFAMHNIAACWLEKKGQDEIFGDGPDVLVASSVAPIGALAPV
                     DGGFRLAGKWSFSSGVEHASWIIVGATLKSESPSAPPDYVFCFVRASDVSVVDDWHVA
                     GLRATGSKSVELNDVLIPRHRVVLLKTVQDGTAPGLAIHRQPFYRLPWYPVFICAFPA
                     VGLGAALAMLEGFRAHIRARVDSFSGIAFQTTSGSAVRMAAAAAELDAARLVFRRDVA
                     ALDRAAQEDRPLRPGESERIMYDAPFIMDVCSRAALELLRGSGGKVLYEANPLQRHFR
                     DIHAMTQHVAMDLDRAGETYGRLLFHNAELALGARDTPHKT"
     gene            1316..>2277
                     /locus_tag="VFL84_03175"
     CDS             1316..>2277
                     /locus_tag="VFL84_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7607653.1"
                     /translation="MRYELPASEDRAIWDIWLSAHRLPAMAVADELGVFAALDSTPAS
                     AAELAQQLGFNRRATDVLLSMLTALGLVTARGGRYELADVTRTYLLPQSPYYWGPLLR
                     VLGVVREQHEALIRALRAAEDLVAPMDLAAAPKDASPDDPAEAWTRGQIDKAQAEAVT
                     QLMHCHSLPASVGVARNGRLEGVSRLLDVGGGSGCFSIAIAQQFPSVRCTVLELPAVC
                     DAARRYINEGGVADRVDTLSIDMFRDAWPRGYDGVFFSNIFHDWNAETNVFLARRAHE
                     ALEPGGRIFLHEQLLAEDGSGPVTTAAFSMLMLLGTHGRQYTFG"
BASE COUNT          345 a          780 c          775 g          377 t
ORIGIN      
        1 cgccaagcaa taccaagaac atcggcgcat cggccgacga gaccgcagac accgtgccgc
       61 tgatgagctt cgagcggcgc gagcgcgagc gcaccgtcgt cgatcacacg ctgacgggcg
      121 tgaggccgat ccgtcaggcc atcctcgatc tctctgccga cgtgcggcaa cgagccgacg
      181 ccgcggagaa agcgcggcaa gttccggccg agacgatggc gagcctgcgc aaagccgggc
      241 tctttcgcgc gttcgtgccg cgcgtctacg gcggcgacga gcgatctctg accgaggtgc
      301 tcgatgcgat gacggagctc gcggccgcgt gtccgtcgac ggcgtgggtc ggcacgctgt
      361 tcgcgatgca caacatcgcg gcctgctggc tcgagaagaa aggccaggac gagattttcg
      421 gcgacggccc ggacgtgctc gtcgcgtcgt cggtcgcgcc gatcggagct ctcgcgccgg
      481 tcgacggcgg tttccggctc gcgggaaaat ggtcgttctc gagcggcgtc gagcacgcgt
      541 cgtggatcat cgtcggcgcg acgctgaaat cggagtcccc gagtgccccg cccgactacg
      601 tcttctgctt cgttcgcgct tcggacgtct cggtcgtcga cgactggcac gtagcgggat
      661 tgcgggcgac gggcagcaag tcggtcgagc tcaacgacgt gctcatcccg cgtcaccgcg
      721 tggtgctgct gaagaccgtg caagacggaa cggcgccggg cctcgccatt cacaggcaac
      781 cgttctatcg cttgccgtgg tatcccgtgt tcatctgcgc gttcccggcc gtcggattgg
      841 gggcggcgct cgcaatgctc gaaggttttc gcgcccatat ccgtgcgcgc gtcgacagct
      901 tctcgggcat cgcattccaa acgacgtccg gctcggcggt gcgcatggcc gcggccgcag
      961 ccgagctcga cgccgcgcgc ttggtcttcc gccgcgacgt ggccgcgctc gatcgcgcgg
     1021 cacaagagga ccggccgctg cgtcccggcg agagcgagcg gatcatgtac gacgcgccgt
     1081 tcatcatgga cgtctgcagc cgcgccgcgc tcgagctcct tcgcggcagc gggggcaaag
     1141 tgctgtatga ggcgaatccg ctgcagcgcc acttccgcga catccacgcg atgacccagc
     1201 acgtggcgat ggacctcgac cgcgccggcg aaacctacgg ccgtttgctc tttcataacg
     1261 ccgagctcgc gctcggcgcg cgcgacactc ctcacaagac ctagtcgaga gcacaatgcg
     1321 ttacgagctt cccgcatccg aagatcgggc gatctgggac atctggctct cggcgcaccg
     1381 gctgcccgcg atggccgtcg cggatgagct cggcgtgttc gccgcgctcg actcgacgcc
     1441 ggcgagcgcg gccgagctcg cgcaacagct cggcttcaat cgacgcgcga cggacgtcct
     1501 gctctcgatg ctgacggcgc tcggcctcgt cacggctcgc ggcggccgct acgagctcgc
     1561 cgacgtcacg cgcacttatc tgctgccgca gagcccgtat tactgggggc cgctgctgcg
     1621 cgttctcggc gtggttcggg agcagcacga ggcgttgatc cgcgccctgc gcgccgccga
     1681 ggacctcgtc gcgccgatgg acctcgccgc ggctccgaag gacgcgagcc ccgacgatcc
     1741 cgcggaagca tggacgcgcg gccagatcga caaggcgcag gccgaggccg tgacgcagct
     1801 catgcattgt cattcgttgc cggcgtccgt cggcgtagcg aggaacggca gattggaagg
     1861 cgtgtcgcgg ttgctcgacg tgggcggcgg ctccggctgc ttctcgatcg cgatcgccca
     1921 acagttcccg tcggtccgct gcacggtgct cgagcttccg gcggtctgcg acgccgcgcg
     1981 ccgctacatc aacgaaggtg gcgtcgcgga tcgcgtcgac acgctatcga tcgacatgtt
     2041 ccgtgatgcg tggccgcgcg gctacgacgg cgtgttcttc tcgaacatct tccacgattg
     2101 gaacgccgag acgaacgtgt ttctcgcgcg gcgcgcgcac gaagcactcg agccgggcgg
     2161 gcgcatcttc ctgcacgagc agctgctcgc cgaggacggc tcgggaccgg tcacgaccgc
     2221 ggcattctcg atgttgatgc tgctcggcac gcacggccgc cagtacacgt tcggcga
//
LOCUS       DASSNN010000177         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_164678, whole genome shotgun sequence.
ACCESSION   DASSNN010000177 DASSNN010000000
VERSION     DASSNN010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164678"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFL84_03180"
     CDS             <1..649
                     /locus_tag="VFL84_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HET7607654.1"
                     /translation="TRVLARADQNPLGSAAGYGVPNLPLDREATSAALGFPRTQEPVT
                     AVQLSRGKAEAALVFEIAVLLGDLGRLASDLLLFYTSEFAYVSLPAEMTTGSSIMPQK
                     RNPDVFELVRGAQATALGALQEILGITAKLPSGYHRDLQRIKAPLFRSIDLAADVLAI
                     VAHALPNVRFRPENIELPPELYAAEQANELVVKQGVPFREAYRRIAALLKRKDES"
     gene            646..816
                     /locus_tag="VFL84_03185"
     CDS             646..816
                     /locus_tag="VFL84_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HET7607655.1"
                     /translation="MRPRVALLAAFVASTVAGCGQTGPLTLPENQRPIERADLPAQPE
                     QQPSDDERQDER"
     gene            806..>2775
                     /gene="polA"
                     /locus_tag="VFL84_03190"
     CDS             806..>2775
                     /gene="polA"
                     /locus_tag="VFL84_03190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET7607656.1"
                     /translation="MSDSPLVLVDGSSYLYRAFHALPPLSTAAGQPTGAVLGVVNMLY
                     KLLDELKPTRMAVVFDAPGKTFRDDLYAEYKADRPPMPDELRAQVEPLNEAIKSMGIC
                     LLRIDGVEADDVIGTLARQAAAAGVSTVISTGDKDLAQLVDEHVTLVNTMDNTTLDRE
                     RVIEKFGVTPEQIVDYLALVGDSIDNIPGIPGVGPKTAVKWLQQYGDLETLKAKAGEV
                     SGKIGDRLREGLSALDLSRQLATIRCDVELPLTIDELALKPQDAERLGALFERLEFTR
                     LLKRVRGGSEPRVSGEPTAAAEPAALATPAGRYETVLTTEALERWGERLAAAPLAALN
                     TETTSLAYMRGELVGIALAVTPGEAAYIPLAHRYPGAPDQLRLEAVLARLKPWLESTA
                     PKVGHHLKYDAHIFANHGITLRGVVHDTMLESYVLNSTATRHELDSVAALYLKLATLK
                     YEDVVGKGSKQIAFDQVDVATATRYSAEAADVALRLHETLWPKLKAVPDLARVYEEIE
                     CPLVRVLKQMEQAGVMVDARLLREQSQELAEKMAAAEKAAHAAAGGPFNLGSPKQLQE
                     VLYERLKLPVLGKTQKGQPSTSEDVLEQLAEEHELPRLILEYRGLTKLKSTYTDKLPT
                     EIDARTKRIHTSYHQTVAATGRLSSSDPNLQN"
BASE COUNT          490 a          941 c          935 g          409 t
ORIGIN      
        1 gacgcgcgtg ctcgcgcgcg cggaccagaa ccctttgggc tccgccgccg gctacggcgt
       61 gccgaatctg ccgctcgacc gcgaagcgac gagcgccgcg ctcggctttc cgcgcacgca
      121 ggagcccgta acggccgtgc agttgtcgcg cggcaaagcc gaggccgcgc tcgtgttcga
      181 gatcgccgtc ctgctcggcg acttgggcag gctcgcgtcg gacttgctcc tgttctatac
      241 gagcgagttc gcgtacgtgt cgctgcctgc cgagatgacg accggctcct cgatcatgcc
      301 gcagaagcgc aaccccgacg tcttcgagct cgtgcgcggc gcgcaggcca cggcgctcgg
      361 cgcattgcag gagattctgg ggatcaccgc gaagctgccg tcgggctatc accgcgattt
      421 gcagcggatc aaggcgccgc tcttccgcag catcgatctc gcggccgacg tgctcgcgat
      481 cgtggcgcac gcgctgccga acgtgcgctt caggcccgag aacatcgagc tgccgccgga
      541 gctctatgcc gccgagcaag cgaacgagct cgtcgttaag caaggggtcc cgttccgcga
      601 ggcgtatcgg cgcatcgccg cgcttctgaa gcgcaaggac gagtcatgag gccccgcgtc
      661 gcgttgctcg cagccttcgt cgcttcgacg gtcgcgggct gcggccaaac ggggccgctg
      721 acgctgcccg agaaccagcg gccgatcgag cgcgccgatc tgcccgcgca gcccgagcag
      781 caaccgagcg acgacgagcg ccaggatgag cgatagtccg ctcgtcctcg tcgacggctc
      841 gtcgtatctg taccgcgcgt ttcacgcgct gccgccgttg tcgacggcgg caggccagcc
      901 gacgggcgcc gtgctcggcg tcgtcaacat gctctacaag ctcctcgacg agctgaaacc
      961 tacgcgcatg gccgtcgtct tcgacgcgcc cggcaagacg ttccgcgacg acttatacgc
     1021 cgaatacaag gcggaccgtc cgccgatgcc ggacgagctc cgcgcgcaag tcgagccgct
     1081 gaacgaggcc atcaagagca tgggtatctg cctgctgcgc atcgacggcg tcgaggccga
     1141 cgacgtcatc ggcacgctcg cgcggcaggc cgcggccgcg ggcgtatcga ccgtgatctc
     1201 gacgggcgac aaggaccttg cccagctcgt cgacgagcac gtgacgctcg tcaacacgat
     1261 ggacaacacg acgctcgatc gcgagcgcgt gatcgagaag ttcggcgtga cgcccgagca
     1321 gatcgtcgac tacctagcgc tcgtcggcga ctcgatcgac aacatcccgg gcattccggg
     1381 cgtcggcccg aagacggccg tcaagtggct ccagcaatac ggcgacctcg agactttgaa
     1441 agcgaaggcc ggcgaggtca gcggcaagat cggcgaccgc ctgcgtgaag ggttgagcgc
     1501 gctcgacctg tcgaggcagc tcgcgacgat tcgttgtgac gtggagctgc cgttgacgat
     1561 cgacgagctc gcgctgaagc cgcaagacgc cgagcggctc ggcgcattgt tcgaacggct
     1621 cgaattcacg cgcttactca agcgagtgcg cggagggagc gagcctcgag tgtccggcga
     1681 gcccacggct gcagcggaac ccgccgcgct cgcgaccccg gccgggcgct acgagaccgt
     1741 cctcacgacg gaggcgctcg agcgctgggg cgagcgcctc gcggcggcgc cgctcgccgc
     1801 gctgaacacg gagacgacga gtctcgccta catgcgcggt gagctcgtcg gcattgcgct
     1861 ggccgtgact ccgggggaag ccgcgtacat cccgctcgcg catcgctatc ccggagcccc
     1921 ggatcagctc cgcctcgaag ccgtgctggc gcggctcaaa ccgtggctcg aaagcaccgc
     1981 accgaaggtc ggccaccact tgaagtacga cgcgcacatc tttgcgaatc acggcatcac
     2041 gctgcgcggc gtcgtgcacg acacgatgct cgagtcgtac gttctgaact cgacggcgac
     2101 gcggcacgag ctcgattccg tcgcggcgct ctatctgaag ctcgcgacgc tgaagtacga
     2161 ggacgtcgtc ggcaagggct cgaagcagat cgcgttcgac caagtcgacg tggcgacggc
     2221 gacgcgctac tcggccgagg ccgccgacgt cgctttgcgg ctgcacgaga cgttgtggcc
     2281 gaagctcaag gccgtgccgg acctcgcgcg cgtctacgag gagatcgagt gcccgctcgt
     2341 gcgcgtgctc aagcaaatgg agcaggccgg ggtcatggtc gacgcgcgcc tgctgcgcga
     2401 gcagagccaa gagctcgcgg agaagatggc ggccgccgaa aaggccgcgc acgccgcggc
     2461 cggcgggccg ttcaacctgg gctcgccgaa gcagctgcag gaagtcctgt acgagcggct
     2521 gaagctgccg gtgctcggca agacgcaaaa gggccagccg tcgacatccg aagacgtgct
     2581 cgagcagctc gcggaagagc acgagctgcc gcgtttgatc ctcgagtacc gcgggctcac
     2641 gaagctgaag tcgacctaca cggacaagct gccgaccgaa atcgacgcgc gcacgaagcg
     2701 cattcatacg tcgtatcacc aaaccgtggc ggcgacgggc aggctatcgt cgtccgatcc
     2761 gaatttgcag aacat
//
LOCUS       DASSNN010000178         6789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1550126, whole genome shotgun sequence.
ACCESSION   DASSNN010000178 DASSNN010000000
VERSION     DASSNN010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550126"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..2029
                     /gene="tkt"
                     /locus_tag="VFL84_03195"
     CDS             2..2029
                     /gene="tkt"
                     /locus_tag="VFL84_03195"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7607657.1"
                     /translation="MALRTDNPTAGARPSRRRLANAIRMLSMDAVEAAKSGHPGMPMG
                     MADIAEVLWNDYLRFNPANGHWHGRDRFVLSNGHGSMLLYSVAYLTGYPIGLDEIKKF
                     RQLGSRAAGHPERDLDIGIETTTGPLGQGLANAVGMALTEKHLAAEFNRPGHDVVDHY
                     TYVFMGDGCLMEGISHEACSFAGTHKLGKLIGFYDDNGISIDGETRGWFTDDTPKRFA
                     AYGWHVVPNVDGHDADAVRRAIDAARAERERPSLICCKTIIGYGAPHVQGTSATHGAA
                     LGAAEAAAARKNLGWDEPPFVIPADVKAAWDARERGARLEAEWSTRFAAYRKQHPELA
                     DELERRMSGKLPAKWSEHAEKAIETLAASGKPMATRKSSQAALEAFAPALPELVGGSA
                     DLTGSNNTQHKFSKPLTPSDASGNYLFYGVREFGMAAMMNGQAAHGGVIPYGGTFLVF
                     SDYARNALRMSALIGLRSIFVLTHDSIGLGEDGPTHQPIEHLASLRLMPNMDLWRPCD
                     TVETAVAWRAAIERGNGPTCLALTRQDLPHVTRTEEQIAMIARGAYIVSNGNEAPAIT
                     LIATGSEVQLAIGAARELEKAGVSARVVSMPCTQAFDRQPAEYRDHVLPPNGARLVVE
                     AGATGGWWRYVGARGAVVGLDRFGHSAPAKALFEHFGFTVEAVVAAAKRLL"
     gene            2065..3078
                     /gene="gap"
                     /locus_tag="VFL84_03200"
     CDS             2065..3078
                     /gene="gap"
                     /locus_tag="VFL84_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET7607658.1"
                     /translation="MPVKVAINGYGRIGRNILRALYESKRTKEINIVAVNDLGDAKTN
                     AHLTKYDTAHGRFPLPVGVDGDSLVVGDDRIKVCAERDPSKLPWGQLGVDIVLECTGL
                     FTTKEKASMHLKAGAKRVIISAPGGKDVDATIVYGVNHKTLKASHTVISNASCTTNCL
                     APLAKVLHESVGIVRGIMTTVHAYTNDQVLTDVYHSDLRRARSATQNMIPTKTGAAAA
                     VGLVLPELDGKLDGFAVRVPTINVSLVDLTFEAGRATSAKEIDEAVKKASQGALKGVL
                     EFVDEPLVSSDFNHNPASSLYDSSLTKVIEGKLVKACAWYDNEWGFSNRMLDTTLAFA
                     AAK"
     gene            3086..4270
                     /locus_tag="VFL84_03205"
     CDS             3086..4270
                     /locus_tag="VFL84_03205"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET7607659.1"
                     /translation="MALKVVRMTDVALKGKRVLIREDLNVPIKDGKVTSTARIEAALP
                     TLRLAVDAGARVMVMSHLGRPKEGKPDPAASLKPVAAKLGELLGRNVPLVTDWLNGVE
                     VAPGELVLLENVRFNVGEEADDDGLAKRMAALCDVFVMDAFGTAHRAQASTNGVARHA
                     PIACAGPLLVAELEALGKALENPKRPLAAIIGGSKVSTKIGVLQSLSTKVDELILGGG
                     IANTILAAGGVNVGKSLYEKDMLDFAAKLLRGEFGKAKIPLPTDVVVAKSLDAGVKGR
                     TTSVREVAADEMILDVGPATAHLYAERLKSAGTIVWNGPLGVFENPEFAAGTRRVAEA
                     IAASGAFSIAGGGDTLAAIEQFGVGDRISYISTGGGAFLEFLEGKKLPAVATLEQRAA
                     GG"
     gene            4273..5550
                     /locus_tag="VFL84_03210"
     CDS             4273..5550
                     /locus_tag="VFL84_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HET7607660.1"
                     /translation="MPPRVAAQRSVLEQAFQHALQAAQPAVCLPPALSSLPRRPALVL
                     GAGKAAASMAAAFCAHWDHPKRGMVVTRYGHGLRDGEDADGLEIVEAGHPSPDSASLA
                     AGKRLLTLAAQLRAGEMLVCLISGGGSSLAAQPLPTLSFEQKRAAANFLIRQGADIRE
                     INCVRKHLSCLKGGRLAAVAHPSPVATFAISDVPGDDVGAIASGPTVPDPTTQADATR
                     ILEKYRYPERAELAPTLSDPKWETPKPGDPKFANDSVHLIATAATALAAAAGFLEREG
                     YRVVQLGDDLDDEAQALGRAHARLAKETRKSGRRTALLSGGETRVVLGDGGGRGGRNT
                     EYLAALALELDGAPGIAALAADTDGIDGHGDHAGAIVVPETLALGAPRDSRLAELLAR
                     HDTYSYFDACGLLLKTGPTRTNVNDFRLILCEP"
     gene            5571..>6789
                     /gene="pyk"
                     /locus_tag="VFL84_03215"
     CDS             5571..>6789
                     /gene="pyk"
                     /locus_tag="VFL84_03215"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET7607661.1"
                     /translation="MIPNGARDDVRRRTKIVATLGPATDDASVIEQLILAGTDVFRIN
                     FSHGTAAEQEARVARVLEVAQRVGRHIGIMGDLQGPKIRVESFKTGSVVLVEGHEFIL
                     DVAMDPHAGDEHAVGCAYRNLTSDVSAGDTLLLDDGLIVLEVREVEGTRIHCIVKQGG
                     RLADHKGLNKQGGGLSAPALTDKDRRDIETAARLKLDFMSVSFARSAEDIEEARRLLR
                     AAGGQGHIVAKIERAEAVTNIRAIIDASDVIMVARGDLAVEVGYASMTGLQKGLIRLA
                     RARNKVCITATQMLESMIHSPSPTRAEVSDVANAVMDGTDAVMLSAETAVGKYPVRAV
                     RTMAELCIGAEKHETFRRAANYRLEDVFTHVDEAIAMAVMYVANHLDVTAIIALTESG
                     STPLWMSRVRSDIP"
BASE COUNT         1156 a         2310 c         2319 g         1004 t
ORIGIN      
        1 gatggcactg cgcactgata acccgactgc cggagcacgg ccctcgcgtc gccggctcgc
       61 gaatgccatt cgcatgttgt ccatggacgc cgtcgaggcc gcgaagtccg gccaccccgg
      121 catgccgatg ggcatggccg acatcgccga ggtgctctgg aacgactact tgcggttcaa
      181 tccggccaac ggccattggc acggtcgcga ccggttcgtg ctgtcgaacg gccacggctc
      241 gatgctgctc tactccgtcg cgtacctgac gggttatccg atcgggctcg acgagatcaa
      301 gaagttccgc cagctcggct cgcgcgcggc cggccatccc gagcgcgacc tcgacatcgg
      361 catcgagacg acgacggggc cgctcggcca aggcctcgcc aatgccgtcg gcatggcgct
      421 caccgagaag cacctcgcgg ccgagttcaa tcgccccggc cacgacgtcg tcgatcacta
      481 cacgtatgtc ttcatgggcg acggctgctt gatggaaggc atctcgcacg aggcgtgctc
      541 gttcgccggc acgcacaagc tcggcaagct gattgggttc tacgacgaca acggcatctc
      601 gatcgacggc gaaacgcgcg gctggttcac cgacgacacg ccgaagcgct tcgcggcgta
      661 cggctggcac gtcgtgccga acgtcgacgg ccacgacgcc gacgccgtgc ggcgcgcgat
      721 cgacgcggcg cgtgcggaga gagagcggcc gtcgctgatc tgctgcaaga cgatcatcgg
      781 ttacggcgcg ccgcacgtgc agggcacgtc ggcgacgcac ggcgcggcgc taggcgccgc
      841 cgaggccgcg gcggcgcgca agaatttggg ctgggacgag ccgcccttcg tgattcccgc
      901 agacgtgaag gccgcctggg atgcgcgcga gcgcggcgcg cggctcgaag cagagtggtc
      961 gacccggttc gcggcctacc gcaagcagca ccccgagctc gctgatgagc tcgagcggcg
     1021 catgagcggc aagctgcccg cgaagtggag cgagcacgcg gagaaagcca tcgagacact
     1081 cgccgcgtcg ggcaagccga tggccacgcg caagtcgtcg caagcggcgc tcgaggcatt
     1141 cgccccggcg ctgcccgagc tcgtcggcgg ctcggccgac ctcacgggct cgaacaacac
     1201 gcagcacaag ttctcgaagc cgctgactcc gagcgatgcg tccggcaatt acttgttcta
     1261 cggcgtgcgc gagttcggca tggccgcgat gatgaatggc caggccgcgc acggcggcgt
     1321 cattccgtac ggcggcacgt tcctcgtgtt ctccgattac gcgcgcaacg cgttgcgcat
     1381 gtccgcgctg atcggactgc gcagcatctt cgtgctgaca cacgactcga tcggcctcgg
     1441 cgaggacggg cccacgcatc agcccatcga gcatctcgcg agcttgcggc tcatgccgaa
     1501 catggatctc tggcggccgt gcgacaccgt cgagacggcc gtcgcgtggc gcgccgcgat
     1561 cgaacgaggg aacggcccga cgtgcctcgc gctgacgcgc caagacctgc cgcacgtgac
     1621 gcggaccgag gagcagatcg cgatgatcgc gcgcggcgcg tatatcgtct cgaacggcaa
     1681 cgaggcgccg gcaataacgc tcatcgcgac gggctccgag gtgcagctcg cgatcggcgc
     1741 ggcgcgcgag ctcgagaagg cgggcgtttc cgcgcgcgtc gtctcgatgc cgtgcacgca
     1801 agcattcgac cggcagcccg ccgagtaccg cgaccacgtc ttgccgccga acggcgctcg
     1861 gctcgtcgtc gaagccggag ccacgggcgg ctggtggcgc tatgtcggcg cgcgcggcgc
     1921 cgtcgtcggt ctcgaccgct tcggccactc ggcgcccgcg aaggcattgt tcgagcattt
     1981 cggcttcacc gtcgaagccg tcgtcgcggc cgccaagcgg ttgctctagt taaattacgt
     2041 cctggcctgc caagggaggc agtcatgccc gtcaaagtcg caatcaatgg ttatggccgc
     2101 atcggccgca acatccttcg cgcgctctac gagagcaagc gcacgaagga aatcaacatc
     2161 gtcgccgtca acgatctcgg cgacgccaag acgaacgcac acctgaccaa atacgacacg
     2221 gcgcacggcc ggttcccgct gcccgtcggc gtcgatggcg actcgctcgt cgtcggcgac
     2281 gaccgcatca aggtctgcgc cgagcgcgat ccgtcgaagc tgccgtgggg ccagctcggc
     2341 gtcgacatcg tcctcgagtg cacggggctc ttcacgacga aagagaaggc ctcgatgcat
     2401 ctgaaggccg gcgccaagag agtcatcatc tcggccccgg gcggcaaaga cgtcgacgcg
     2461 acgatcgtct acggcgtcaa ccacaagacg ctgaaggcga gccataccgt gatctcgaat
     2521 gcgtcgtgca cgacgaattg cttggcgccg ctcgcgaagg tgctgcacga gagcgtcggc
     2581 atcgtgcgcg gcatcatgac cacggtgcac gcctacacga acgaccaggt gctgacggac
     2641 gtctatcaca gcgacctgcg ccgcgcgcgc tccgcaacgc agaacatgat cccgacgaaa
     2701 acgggcgccg ccgcggccgt cggcctcgtg ctgcccgagc tcgacggcaa gctcgacggc
     2761 ttcgccgtgc gcgtgccgac gatcaacgtc tcgctcgtcg atctcacgtt cgaagctggc
     2821 cgcgcgacga gcgcgaaaga gatcgacgag gccgtcaaga aggcgagcca gggcgcgctc
     2881 aagggcgtgc tcgagttcgt cgacgagccg ctcgtctcga gcgacttcaa tcacaacccc
     2941 gcgtcttcgc tctacgattc gagcctgacg aaggtcatcg aaggcaagct cgtcaaagcc
     3001 tgcgcgtggt acgacaacga gtggggcttc tcgaatcgca tgctggacac gacgctcgcg
     3061 ttcgccgccg cgaagtagcg ccgccatggc cctcaaggtc gtccgcatga cggatgtcgc
     3121 gctgaagggt aagcgcgtgc tgattcgaga agacctcaac gttcccatca aggacggaaa
     3181 ggtcacgagc acggcgcgca tcgaggcggc gctgccgaca ttgaggctcg cggtcgacgc
     3241 cggcgcgcgg gtcatggtca tgtcgcatct cggcaggccc aaagaaggca agcccgatcc
     3301 ggcggcgtcg ctgaagcccg tcgccgcgaa gctcggcgag ctgctcggcc gcaacgtgcc
     3361 gctcgtgacc gattggctga acggcgtcga ggtcgcgccc ggcgagctcg tgctcttgga
     3421 gaacgtgcgg ttcaacgtcg gcgaggaagc cgacgacgac ggcctcgcga aacgcatggc
     3481 cgcgctttgc gacgtgttcg tcatggacgc gttcggcacg gcgcatcgag cgcaggcgag
     3541 cacgaacggc gtggcgcgcc atgcgccgat cgcgtgcgcg gggccgctgc tcgtcgcgga
     3601 gctcgaggcg ctcggcaagg cgctcgagaa cccgaagcgc ccgctcgccg cgatcatcgg
     3661 cggctcgaag gtctcgacga agatcggagt gctgcagtcg ctgtcgacga aggtcgacga
     3721 gctgatcttg ggcggcggca tcgcaaatac gattctcgcg gccggcggcg tcaacgtcgg
     3781 caagtcgctc tacgagaagg acatgctcga cttcgccgcc aagctgctgc gcggcgagtt
     3841 cggcaaggcg aagatcccgc tgccgacgga cgtcgtcgtg gcgaagagcc tcgatgccgg
     3901 cgtcaagggc cgcacgacga gcgtgcgcga ggtcgccgcg gacgagatga tcctcgacgt
     3961 cggccccgcg acggcgcacc tctacgccga gcgcttgaag agcgccggca cgatcgtctg
     4021 gaacggcccg ctcggcgtgt tcgagaaccc cgagttcgcg gccggcacgc gccgcgtcgc
     4081 agaggcgatc gccgcgagcg gcgcgttctc gatcgcgggc ggcggcgaca cgctcgcggc
     4141 catcgagcag ttcggcgtag gcgaccgcat ctcgtacatc tcgacgggcg gcggcgcgtt
     4201 cctcgaattc ctcgagggca agaagctgcc cgccgtcgcg acgctggagc agcgagcggc
     4261 cggaggctga cggtgccgcc tagagtcgcc gcgcaacgct cggtgctcga gcaggcgttt
     4321 cagcacgcgt tgcaagcggc gcagcccgcc gtctgcttgc cgcccgcgct ctcgagcttg
     4381 ccgcgccggc ccgcgctcgt gctcggcgcc ggcaaggccg ccgcgtcgat ggcggctgcg
     4441 ttctgcgcgc actgggacca ccctaagcgc ggcatggtcg tgactcgcta cggccacggg
     4501 ctcagggatg gcgaagacgc ggacggcttg gagatcgtcg aggcgggcca tccatcgccg
     4561 gacagcgcga gcctcgcggc gggcaaacgg ctgctcacgc tcgcggcgca gctccgcgcc
     4621 ggcgagatgc tcgtctgtct catctcgggc ggcggctcgt cgctcgcggc gcagccgctt
     4681 ccgaccctct cattcgagca gaagcgcgcc gcggccaatt ttctgattcg ccaaggcgcc
     4741 gacatccgcg agatcaattg cgtgcggaag cacttgtcgt gcttgaaggg cgggcggctc
     4801 gcggccgtcg ctcatccgag tcccgtcgcg acgttcgcga tctcggatgt gcccggcgac
     4861 gacgtcggcg cgatcgcctc ggggccgacc gtgccggacc cgacgactca ggccgacgcg
     4921 acgcgcatcc tcgagaagta tcggtatccc gagcgcgccg agctcgcgcc gacgttgagc
     4981 gatccgaagt gggagacgcc gaagcccggc gatccgaagt tcgcgaacga cagtgtgcac
     5041 ctgatcgcga cggccgcgac ggcgctcgcg gccgccgcgg gttttctcga gcgtgaaggc
     5101 tatcgcgtgg tgcagctcgg cgacgacctc gacgacgaag cgcaagcgct cggccgcgcg
     5161 cacgcgcggc tcgcgaagga aacgcgcaag agcggccggc gcacggcgct tctgtccggc
     5221 ggcgagacgc gcgtcgttct cggcgacggc ggcgggcgag gcggccgcaa cacggagtat
     5281 ctcgccgcgc tcgcgctcga gctcgacggc gcgcccggca tcgcggccct cgccgcggac
     5341 acggacggca tcgacgggca cggcgaccac gccggcgcaa tcgtcgtgcc ggaaacgctc
     5401 gctctcggcg cgccgcgaga ctcacgtctc gccgagctac tggcgcggca tgatacttat
     5461 agttactttg atgcctgcgg tcttttgctg aagacggggc ccactcgtac caacgtcaac
     5521 gatttcagat taatactttg cgaaccctaa ctcgttccgg cgattccacg ttgattccca
     5581 atggtgcgcg cgacgacgtt cgtcgccgca cgaagatcgt cgcgacgctc ggcccggcga
     5641 ccgacgacgc cagcgtcatc gagcagctga tcctcgcggg caccgacgtg ttccgcatca
     5701 acttctcgca cggcacggcg gcagagcaag aggcgcgtgt ggcgcgagtg ctcgaggtcg
     5761 cgcaaagggt cggccgccac atcggcatca tgggtgacct gcaggggccg aagatccgcg
     5821 tcgagtcgtt caagacgggc tccgtcgtgc tcgtcgaagg tcacgagttc atcctcgacg
     5881 tcgcgatgga cccgcacgcc ggcgacgagc atgcggtcgg ctgcgcgtat cgcaacctca
     5941 cgagcgacgt cagcgccggc gacacgctgc tgctcgacga cggcctcatc gtgctcgaag
     6001 tacgagaggt cgaaggcacg cgcatccatt gcatcgtcaa gcagggcggc cgcttggccg
     6061 atcacaaggg cttgaacaag cagggcggcg gcttgtccgc gcccgcgctc accgacaaag
     6121 acaggcgcga catcgaaacg gccgcgcgcc tgaagctcga cttcatgtcg gtgtcgttcg
     6181 cgcgcagcgc cgaagacatc gaggaggcgc ggcgcctgct gcgcgcggcc ggggggcagg
     6241 gccatatcgt cgcgaagatc gagcgcgccg aagccgtcac gaacattcgc gcgatcatcg
     6301 acgcgagcga cgtcatcatg gtggcgcgcg gcgatctcgc cgtcgaagtc ggctacgcat
     6361 cgatgacggg cctgcagaag gggctgatca ggcttgcgcg cgcgcgcaac aaggtctgca
     6421 tcacggcgac gcagatgctc gaatcgatga tccattcgcc gtcgccgacg cgcgccgagg
     6481 tctcggacgt cgcgaacgcc gtcatggacg gcaccgacgc cgtcatgctc tcggccgaga
     6541 cggccgtcgg caagtatccc gtgcgcgccg tgcgcacgat ggccgagctc tgcatcggcg
     6601 cggagaagca cgagacgttc cgcagggcgg ccaactatcg cctcgaggac gtgttcacgc
     6661 acgtcgacga ggcgatcgcg atggccgtca tgtacgtggc gaaccacctc gacgtcacgg
     6721 cgatcatcgc cctcacggag tcgggctcga cgccgctctg gatgtcgcgc gtgcgttccg
     6781 acattcccg
//
LOCUS       DASSNN010000179         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_901991, whole genome shotgun sequence.
ACCESSION   DASSNN010000179 DASSNN010000000
VERSION     DASSNN010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901991"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFL84_03220"
     CDS             <1..502
                     /locus_tag="VFL84_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7607662.1"
                     /translation="YAQTNVTVTPTVTSIQNVDEIRTSGLEVAYRSGPLLGDRLEVSA
                     SVTYAHSRIEENANFPASVGKWQPRVPDWRANAFATYRFGDSWSLTAGGRYSGSQYNT
                     LDNSDTNPFSFTGTSPFIVFDLRARYANDRWSAAFGIDNAGDEEYWAFHPYTRRSLIA
                     EFGVGF"
     gene            complement(499..1530)
                     /locus_tag="VFL84_03225"
     CDS             complement(499..1530)
                     /locus_tag="VFL84_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET7607663.1"
                     /translation="MANGASLAELRDGLRAAGARPRHETLMLRAWVRGLPLDAFAQRE
                     DAQLPLTLRRALPKLAQRFASLVDVLSEHRGADASRRLLLRLEDGKTIESVLLPGDGV
                     CVSTQVGCAVGCTFCMTGRDGLMRQLTSGEIVAQVALARSRQRVRRVVFMGMGEPAHN
                     LDNVLDAIALLGAEGDLAQKNLVFSTVGDRRVFERLPLCSIKPALALSLHTTKPELRA
                     QLLPRAPRVEPAELVSLADAYSRATKYPTQYQWTLLEGVNDGDDELDALAALLEGHYA
                     VVNFIPYNTIDGVNFRRPSWTRAAEMTRYLHRRGVLAKLRRSAGQDVDAGCGQLRARH
                     ARIADALGA"
     gene            2058..>2630
                     /locus_tag="VFL84_03230"
     CDS             2058..>2630
                     /locus_tag="VFL84_03230"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HET7607664.1"
                     /translation="MKRQPRPPRLSPLAGAIALATGVSLLLQSSYVRAQEPEQGNDLE
                     EITVTGTRIQRTSGFATPVPVTAVSLEEIESFKPGTTMADQLDQLPQFYQTQSAQRGG
                     GALFGSAGGSYLDLRAMGPARTLILLDGARVTPADRTGVVNVDNFPTALLRSVEVVTG
                     GASAAYGADALAGVTNFNLNRDFRGVNLSFD"
BASE COUNT          441 a          891 c          871 g          427 t
ORIGIN      
        1 gtacgcgcag acgaacgtta cggttacgcc gaccgttacg agcatccaga acgtcgacga
       61 gattcggacg agcggtctcg aggtcgcgta tcggtcgggg cccttgctcg gcgaccggct
      121 cgaagtgtcg gcgagcgtga cgtacgctca ctcgcgcatc gaggagaacg ccaattttcc
      181 cgcgagcgtc ggcaagtggc agccgcgcgt ccccgattgg cgcgcgaacg ctttcgcgac
      241 ctaccgcttc ggcgacagct ggtcgctgac ggcgggcggc cgctacagcg gctcgcagta
      301 caacacgctc gacaattccg acacgaaccc gttcagcttc acgggcacga gcccgttcat
      361 cgtgttcgac ttgcgcgcgc gttacgcgaa cgaccgatgg agcgccgcgt tcggcatcga
      421 caacgcgggc gacgaggaat actgggcgtt ccacccttac acgcggcgca gcctgatcgc
      481 ggaattcggc gtcggattct aggcgccgag cgcgtcggcg atgcgcgcat gccgggcgcg
      541 gagttggccg cagccggcgt cgacgtcctg gcccgcggag cggcgcagct tcgcgagcac
      601 gccgcggcgg tgcagatagc gcgtcatctc ggccgctcgt gtccacgacg gtctccgaaa
      661 attcacgccg tcgatcgtgt tgtacgggat gaagttcacg acggcgtaat gtccctcgag
      721 cagcgccgcg agcgcgtcga gctcgtcgtc gccgtcgttg acgccctcga gcagcgtcca
      781 ctgatactgc gtcggatact tcgtcgcgcg cgagtacgcg tcggcgagcg agacgagctc
      841 ggccggctcg acgcgcggcg cgcgcggcag cagctgcgcc cgcagctcgg gcttggtcgt
      901 gtgcagcgac agcgctagcg ccggcttgat ggagcaaagc ggcaggcgct cgaacacgcg
      961 ccgatcgccg acggtcgaga acacgagatt tttttgcgcg aggtctccct cggcgccgag
     1021 cagagcgatc gcgtcgagca cgttgtccag gttgtgcgcg ggctcgccca tgcccatgaa
     1081 gacgacgcgg cgcacgcgct gccgcgaccg agccagcgcg acttgcgcga ctatctcgcc
     1141 gctcgtcagc tggcgcatga ggccgtcgcg gcccgtcatg cagaacgtgc agccgaccgc
     1201 gcaacccact tgcgtcgaga cgcagacgcc gtcgccgggc agcagcacgc tttcgatcgt
     1261 cttgccgtct tcgagccgca ggagcagccg tcgcgacgcg tccgcgccgc ggtgctcgga
     1321 gagcacgtcg acgagcgatg cgaagcgctg cgcgagcttc ggcaacgcgc ggcgcagcgt
     1381 cagcggcagc tgtgcatcct cgcgttgcgc gaacgcgtcg agcggcaagc cgcgcaccca
     1441 cgcgcgcagc atcaacgtct cgtgccgcgg ccgcgcaccg gcggcgcgca ggccgtcacg
     1501 cagctcggcg agcgaagctc cgttggccac ggctactcaa cgctgcgcaa gcttgatcag
     1561 ctcgatgcga cgcattgtcc tctccattgg aaactggtga cgcaagtccg agccacgata
     1621 tcgacggctc gcaaccttgt aaacggaacc gacaggcttg acgacaacgg gcccgcggta
     1681 ttctgatgtc gctggagccg cggcccggcg ttcgcacgga ccgcttcaac ggtgtgaggg
     1741 ggggatcgac cggcctccgg ctgcaagcgc tgcagccgga ggtcgctgtc gaagcgcgca
     1801 cgacgaaaac gtccagcgat ctgcaccgag acgaacgaac aaagcgcgat cgaggaccgc
     1861 cccagcgcgc gagcattcac gctatcgttc gtcgtgtgcg ttgcctcgcc gccacaagcc
     1921 tgccacgcgt ccagcgcatt cggtgctacc tcccggggcg gtcgcgtttc ccttcttgtc
     1981 aggctagtag ctactgagct agtgtcttcg atactagatc cgtagcgtcc atccaacgga
     2041 tttttttggg gggaatcatg aaacgccaac cgcgtccgcc gcggctttcg ccgcttgccg
     2101 gtgcaattgc tctcgccacc ggagtctcgc tgttgttgca gagcagctac gttcgcgcgc
     2161 aggaaccgga acagggcaac gatctcgagg aaattacggt caccggcacg cgcatccagc
     2221 gcacgtccgg tttcgccacg ccggtaccgg tcaccgccgt ctcgctcgaa gagatcgaat
     2281 cgttcaaacc cgggacgacg atggcggatc agctcgatca gctgccgcag ttctatcaaa
     2341 cgcaaagcgc ccagcgcggc ggcggcgcgt tgttcggcag cgcgggcggc agctatctcg
     2401 acctgcgcgc gatgggtccc gcgcgcacgc tgatcctgct cgacggcgcg cgcgtgacgc
     2461 cggccgatcg caccggcgtc gtcaacgtcg acaatttccc gaccgcgctg ctacgcagcg
     2521 tcgaggtcgt gacgggcggc gcctcggcgg cgtacggcgc cgatgcgctt gcgggcgtaa
     2581 cgaatttcaa cttgaaccgc gacttccgcg gcgtgaatct ctcgtttgac
//
LOCUS       DASSNN010000180         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1280884, whole genome shotgun sequence.
ACCESSION   DASSNN010000180 DASSNN010000000
VERSION     DASSNN010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280884"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFL84_03235"
     CDS             complement(<1..347)
                     /locus_tag="VFL84_03235"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HET7607665.1"
                     /translation="MQPVRFEDGRPMLLAGIRRKHTFASMGVDIPRQWNELEKLGKLP
                     GQVGTTAYGAICGGDARKQTMEYMVGVEVKSFDAVPKELGRMRVPAARYAVFLHEGNV
                     ATIQETWQQILSS"
     gene            complement(416..1525)
                     /locus_tag="VFL84_03240"
     CDS             complement(416..1525)
                     /locus_tag="VFL84_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009803035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET7607666.1"
                     /translation="MALKRPSWPAGRVVTLEHTSKVLADNPLGDPHVRTLDVWLPPQY
                     DGAKGRGLGRRFPVLFDLVGFTGSGRMHTAWRNFDENVPERAARLVHDAKMAPAIIVF
                     PDCFTAFGGNQYVNSPAVGRYADYLTRELVPFVDRELRTLAAREHRGVFGKSSGGYGA
                     LVHAMKYPKCWGAAASHSGDAGFETLYAPEWPSTLDELANYREPPLAAGERDVRAAVK
                     AVGYDGVDDGRVMRFLARMWTKRQPSTAEIHALTNIAMAATYDPDPKALNGFRVPFHF
                     ETGERLPQRWQRWLRHDPVNLVARYAASLRTLRGLSIDCGWRDQYRIHYGSRLLSLRL
                     TEHRVPHVYEEFDGTHSGVDYRMDRSLPFLTHALR"
     gene            complement(1532..3100)
                     /locus_tag="VFL84_03245"
     CDS             complement(1532..3100)
                     /locus_tag="VFL84_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7607667.1"
                     /translation="MTALSHVRAPRPLDTSLPQHESLAHMLLAAVDAHPDLPAIILEQ
                     QRISYAELGRAVAGLASWLAAAGAKRGERVMLLMANSIEMTAALNAVWATGAQVAPIN
                     PFLTPSELKKQLGDVQACAIVSDKLSEQKAAEVAVQFAIPTKLTLGPGGTTLDAWKAD
                     KSLQLDRSRFPKADDFALLIFTGGSTGVPKGVNHTHRALLYSVLQHVTVWPCKFGQER
                     FLNVAPMFHIWGLGYSTLVPIYARSTLVMVPRYDADKVVQGLSDHKITVFAGGPAPIY
                     MGMLASPRFANADLSHLRYCPSGGAPCPAELHREWLEKTGQPILEGWGMTEGAPFCLN
                     VYSGGKRKLLSVGNPVPGTELQVVDLENGDRVLPQGERGELRVRGPQMGAGYRNQPEE
                     TARTYRDGWVHTGDIGYVDEEGFVFLVDRKKDMVIVGGFNVYPREVDEVLFKHPKIRE
                     AATVGKRDPRLGEVLVAFVVLDEGASLSKEEFFEYCKTEMVKYKRPVEVYFVDSLPKT
                     GTNKINRRALRERV"
     gene            complement(3097..>3838)
                     /locus_tag="VFL84_03250"
     CDS             complement(3097..>3838)
                     /locus_tag="VFL84_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET7607668.1"
                     /translation="AVALWPGTGAVLRGLFVPSLPRADAEAVVWTVSLIGGIGGTLTV
                     LSYGYWLREEGRTSPEDLRTCRIDLAASYFMMALFGLFMIIVGQTVKIEGQGTAMLVV
                     LSDRLGEELGPAGKWLFLAGTFGTVFSSLLGVWQATPYLFAECWRLGVRRDAKPVDTS
                     APTYRWFLVVLAFVPMVGLFGSFREVQKIYTFIGAYIFPTLALVLIVFNSRGAWVGQR
                     FKNHPVTIALLAGVLIFFTWLAIENIEA"
BASE COUNT          669 a         1312 c         1258 g          599 t
ORIGIN      
        1 cagctcgaca gaatctgctg ccacgtctcc tgaatcgtcg cgacgtttcc ttcgtggagg
       61 aacacagcgt agcgcgcggc cggcacgcgc atgcggccga gctccttcgg caccgcatcg
      121 aagctcttca cctcgacacc aaccatgtac tccatggttt gtttccgcgc atcgccgccg
      181 caaatcgcgc catacgcggt cgtcccgacc tgtcccggga gcttcccgag cttctcgagc
      241 tcgttccact gacgcgggat gtccacgccc atgctcgcga acgtgtgctt gcggcggatg
      301 ccggccagga gcatcggccg gccgtcctcg aaacgaactg gttgcacgtc gctcactcgc
      361 actccgcggc gcgccgaggc gccgcattca gaatagcgac ggccccaaaa cgcgtctacc
      421 gcagcgcgtg cgtgaggaac ggcaggctgc ggtccatgcg gtaatcgacg cccgagtgcg
      481 tgccgtcgaa ctcctcgtag acgtgcggca cgcgatgctc ggtcaggcgc agcgacaaca
      541 atcggctgcc gtaatgaatg cggtattgat cgcgccagcc gcagtcgatg gagagaccgc
      601 gcagggtgcg caagctcgcg gcgtagcgcg cgacgaggtt gacggggtcg tggcgcagcc
      661 agcgctgcca gcgctgcggc aaccgctcgc ccgtctcgaa atggaacggc acgcgaaagc
      721 cgttcaacgc tttcggatcg gggtcgtacg tcgcggccat cgcgatgttc gtcagcgcgt
      781 gaatctcggc ggtgctcggc tggcgcttcg tccacatgcg agcgagaaag cgcatcacgc
      841 ggccgtcgtc cacgccgtcg tagccgaccg ccttgacggc cgcgcgcaca tcgcgctcgc
      901 cggccgcgag cggcggctcg cgataattcg cgagctcgtc gagcgtcgag ggccactccg
      961 gcgcgtacag cgtctcgaag cccgcgtcgc cggaatgcga agcggccgcg ccccagcact
     1021 tcgggtactt catcgcgtga acgagcgcgc cgtagccgcc cgaggacttg ccgaacacgc
     1081 cgcgatgctc gcgcgcggcg agcgtgcgca gctcgcgatc gacgaatgga acgagctcgc
     1141 gcgtcaggta gtccgcgtag cggccgacgg ccggcgagtt cacgtactga ttgccgccga
     1201 acgccgtgaa gcaatcggga aaaacaatga tcgccggcgc catcttggcg tcgtgaacga
     1261 gccgcgcggc gcgctcgggc acgttctcgt cgaagttgcg ccacgcggta tgcatgcgcc
     1321 ccgagcccgt gaagccgacg agatcgaaca gcacggggaa tcgccggcca aggccgcgac
     1381 ccttcgcgcc gtcgtactgc ggcggcagcc agacgtcgag tgtgcgcacg tggggatcgc
     1441 cgagcggatt gtcggcgagc accttcgacg tgtgctcgag cgtcacgacg cgcccggccg
     1501 gccacgacgg gcgcttcagc gccatggcgc gctagacgcg ctcgcgcagc gcgcggcggt
     1561 tgatcttgtt cgtaccggtc ttcggcagcg agtcgacgaa gtagacctcg acgggccgct
     1621 tgtacttgac catctcggtc ttgcagtact cgaagaactc ttctttcgag agcgacgcgc
     1681 cctcgtcgag cacgacgaac gcgacgagca cctcgcccaa gcgcgggtcg cgcttgccga
     1741 cggtcgcggc ctcgcggatc ttcggatgct tgaacagcac ctcgtcgacc tcgcgcgggt
     1801 acacgttgaa gccgccgacg atgaccatgt ccttcttgcg gtcgacgaga aagacgaagc
     1861 cttcctcgtc gacgtagccg atgtcgcccg tatgcaccca gccgtcgcga tacgttcgcg
     1921 cggtctcttc cggctgattg cggtaacccg cgcccatctg tgggccgcgc acgcggagct
     1981 cgccgcgctc gccctgcggc agcacgcggt ctccgttctc gaggtcgacg acttgcagct
     2041 ccgtgccggg cacgggattg ccgaccgaca ggagcttgcg cttgccgccg ctgtacacgt
     2101 tcaggcagaa cggcgcgcct tccgtcatgc cccagccctc gagaatcggt tggccggtct
     2161 tctcgagcca ttcgcgatgc agctcggcag ggcacggcgc gccgccggac gggcagtaac
     2221 gcaagtgcga gagatcggcg ttcgcgaacc tggggctcgc gagcattccc atgtagatcg
     2281 gcgcggggcc gcccgcgaac accgtaatct tgtgatccga aagcccctgc acgactttgt
     2341 cggcgtcgta gcgcggcacc atgacgagcg tgctccgcgc gtagatcggc accaacgtcg
     2401 agtagccgag accccagatg tggaacatcg gcgcgacgtt caagaatctt tcctggccga
     2461 acttgcacgg ccacaccgtg acgtgctgca gcaccgagta gagcaacgcg cggtgggtgt
     2521 gattgacgcc cttaggcaca cccgtcgagc cgcccgtgaa gatcaagagc gcgaagtcgt
     2581 cggctttcgg gaagcgcgag cgatcgagct gcaacgactt gtcggccttc cacgcgtcga
     2641 gcgtggtgcc gccggggccg agcgtgagct tcgtcggaat ggcgaactgc acggcgactt
     2701 cagccgcctt ctgctcggag agcttgtcgc tgacgatggc gcacgcctgc acgtcgccga
     2761 gctgcttctt gagctccgac ggcgtcagga acggattgat gggcgcaacc tgcgctcccg
     2821 tcgcccaaac ggcgttcaac gccgccgtca tctcgatcga gttcgccatc agcagcatga
     2881 cgcgctcgcc gcgcttcgcc ccggctgccg ccaaccagct cgcgagacct gcgaccgcgc
     2941 ggccgagctc cgcatacgag atgcgctgct gctcgaggat gatcgcgggc aagtcggggt
     3001 gcgcgtcgac ggcggccagg agcatgtgcg cgaggctctc gtgctgcggc aacgacgtat
     3061 ccaatggcct gggagcgcgg acgtgcgaca gggcggtcat gcttcgatgt tctcgatggc
     3121 gagccaggtg aagaaaatta gcacgccggc gagcaaagcg atcgtgacgg gatgattttt
     3181 gaagcgctgg cccacccacg cccctcgcga gttgaagacg atcagcacga gagcgagcgt
     3241 cgggaagata tacgccccga tgaacgtata gatcttctgc acctcgcgga agctgccgaa
     3301 gaggccgacc atcggcacga acgcgagcac gaccaagaac cagcgatacg tcggcgcgct
     3361 cgtgtcgacg ggcttggcat cgcggcgcac gccgagccgc cagcactcgg cgaacaggta
     3421 cggcgtcgcc tgccagacgc cgagcaagct gctgaacacg gtgccgaacg tgccggcgag
     3481 gaacaaccac ttgccggccg ggcccagctc ctcgccgagg cggtccgaca gcacgaccag
     3541 catcgcggtc ccctgcccct cgatcttcac ggtctgcccg acgatgatca tgaagaggcc
     3601 gaacagcgcc atcatgaaat agctcgccgc gagatcgatg cggcacgtgc gcaggtcctc
     3661 cgggctcgtt cgcccttcct cgcgcagcca atagccgtac gacagcacgg tcagcgttcc
     3721 gccgatgccg ccgatcagcg acacggtcca gacgacggcc tccgcgtctg cgcgcggcag
     3781 cgaaggcacg aacagcccac gcagcaccgc gccggtgccg ggccacagcg cgacggcc
//
LOCUS       DASSNN010000181         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1281419, whole genome shotgun sequence.
ACCESSION   DASSNN010000181 DASSNN010000000
VERSION     DASSNN010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281419"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..581
                     /locus_tag="VFL84_03255"
     CDS             66..581
                     /locus_tag="VFL84_03255"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HET7607669.1"
                     /translation="MRILFVCMGNICRSPTAEGVFRKLLAERAPELDVDIDSAGTHGF
                     HDGAPPDPRACRAAERRGIDLKPLRARRVTERDFEEFELVLAMDEQNLEFLLEVCPAE
                     HRHRVRLFLEFAPHLERREVPDPYYGGSTGFEHVLDLVEEAAAGLLEHLRSTPPPRPP
                     SGAAESPRKAD"
     gene            complement(578..>2930)
                     /locus_tag="VFL84_03260"
     CDS             complement(578..>2930)
                     /locus_tag="VFL84_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HET7607670.1"
                     /translation="EISSEYFVKQPDSGEVRIRDVLREGQEIVVQVDKEERGTKGAAL
                     TTFISLAGRFIVLMPNNPGAGGVSRRIQGEERDTVRQSLEDLQVPDGMGCIVRTAGIG
                     RTTEELKWDLDYLLRVWEAIKQVVVTRPAPFLIYQEGNAIVRALRDYFSNDIGEILID
                     NPTIYQEAVEFMERVLPHNIKKLKLYEDNTVPLFTRYQIESQIESAYGHKVQLPSGGS
                     IVIDRTEALTAIDINSARSTKGEDIEETALNTNLEAADEIGRQLRIRDLGGLVVVDFI
                     DMGPQRNQREVEDRLRAAVRHDRARIQIGKISRFGLLEMSRQRLRPSLEESTQSVCPR
                     CSGAGNIRSVESLALAVLRLVGEEARKERTAKVIAQLPMDVANYVLNEKRDWVQTVQE
                     ANGVQVVLLGNPDLDTPNYTLKRVRDDELGLPENAGTSYKLVAPKPDISVAYEEIKKQ
                     PKAEEAAVSNVLPATPAPTPPAPPPVEQAPPAPRPVAQIGIFTRLWVYLFGRGEAAAP
                     ARDGDGGERQRSGRRDFRDRDRERDRDRDFRGRGDHRRDRDRDRDRDRDRDRDRDRGD
                     RNARGRDRDHRDRERNREQRPADAGGSRPRPEPQQGQAPQQGQSSQGGQGQGQPGQQA
                     QQGQQGQPGRGGRRSRHRRGGQRHDHGQQQRPPQANGGQPAQSEASASFSADTNQARA
                     PQGAVAEAPPSTSTPDIGDSGRFETLPDRTPEPEAPRESAPERDYAPPPRPEPVETEH
                     APSAPREEWSPPERGFSDAPAERSDDTSMSESAPVPSDERQAS"
BASE COUNT          364 a         1010 c         1007 g          549 t
ORIGIN      
        1 gccggtcacc acacacagct tgtcgttaag tcccatgtcc atctcaacgg gcattcttcc
       61 gggtcgtgcg catcctgttc gtctgcatgg gcaacatctg ccgctcgccg acggccgaag
      121 gcgtattccg caagctgctc gccgagcgcg cacccgagct cgacgtcgat atcgactcgg
      181 ccggcacgca cggttttcac gacggcgctc ctcccgaccc gcgcgcgtgc cgggccgcgg
      241 agcgccgcgg catcgatctg aagccgctgc gagcgaggcg agtgacggag cgggacttcg
      301 aggagttcga gctcgtgctc gccatggacg agcagaatct cgagttcctg ctcgaggttt
      361 gcccggccga gcaccggcac cgggttcggt tgttcttgga gttcgcgccg catctcgagc
      421 ggcgcgaggt gcccgatccc tattacggcg gcagcacggg cttcgagcac gtgctcgatc
      481 tcgtcgaaga ggccgctgcc ggcctcctcg agcatttgcg cagcacgccg ccgccgcggc
      541 cgccgagcgg cgccgcggaa tcgccgcgca aggccgacta gctcgcctgg cgctcgtccg
      601 agggaacggg cgccgactcg ctcatgctcg tgtcgtcgga acgctccgcc ggcgcgtcgc
      661 tgaaaccgcg ctccggcggc gaccactctt cgcgcggcgc gctcggcgcg tgctccgttt
      721 cgacgggctc cgggcgaggc ggtggggcgt aatcgcgctc gggcgccgac tcgcgcggcg
      781 cctcgggctc gggcgtgcgg tccggcagcg tctcgaagcg tccactgtcg ccgatgtcgg
      841 gcgtcgacgt cgacggcggt gcttcggcaa ccgcgccctg cggcgctcta gcttggttgg
      901 tgtcggcgct gaacgatgcg ctcgcttcgc tttgcgccgg ttgtccgccg ttcgcttgcg
      961 gcgggcgttg ctgctgcccg tgatcgtgcc gttggccgcc gcgccgatgt cgcgagcgcc
     1021 ggccgcctcg accgggctgc ccttgttgac cttgctgtgc ctgctgcccc ggttgccctt
     1081 gtccctggcc tccctgcgag ctttggccct gttgcggtgc ttggccctgt tgtggctcgg
     1141 gacgcggtcg cgagccgccc gcatcagcgg gccgctgctc gcgattgcgc tctctgtcgc
     1201 ggtgatcgcg gtcgcgcccg cgcgcattgc gatcgcctcg gtcgcgatcc cgatcgcggt
     1261 ctcgatcacg atcacgatcg cgatcgcgat cgcggcgatg atcgccgcgg ccgcggaagt
     1321 ctcgatctcg atcgcgttct cggtcgcgat cgcggaagtc gcggcgtccc gagcgctggc
     1381 gctcgccgcc gtcgccgtcc cgcgccggtg cggccgcttc gccgcggccg aagaggtaca
     1441 cccagagccg cgtgaagatg ccgatctggg cgacgggcct tggcgccggc ggcgcctgct
     1501 cgacgggcgg gggtgcgggc ggcgtcggcg ccggcgtggc cggcaggacg ttcgagaccg
     1561 cggcttcttc ggccttcggc tgcttcttga tctcctcgta cgcgacggag atgtcgggct
     1621 tgggtgcgac gagcttgtag ctcgtgcccg cgttctcggg cagcccgagc tcgtcgtcgc
     1681 gcacgcgctt caacgtgtag ttcggcgtat cgagatccgg gttgccgagc agcacgacct
     1741 gaacgccgtt cgcctcttgc acggtctgga cccaatcgcg cttctcgttc agcacgtagt
     1801 tcgcgacgtc catcggcagc tgcgcgatga ccttcgccgt gcgctccttg cgcgcttcct
     1861 cgccgacgag ccgtagaacg gcgagcgcca gcgactcgac gctgcggatg ttgccggcgc
     1921 cgctacagcg cgggcagacc gattgcgtcg actcctcgag cgaaggccgc agccgctgcc
     1981 gcgacatctc gagcaagccg aaccgcgaga tcttgccgat ctggatgcgc gcgcggtcgt
     2041 ggcgcacggc ggcacgcagc cggtcctcga cctcgcgctg attgcgctgc gggcccatgt
     2101 cgatgaagtc gacgacgacg aggccgccta agtcgcggat tcgcagctgc cggccgatct
     2161 cgtcggcggc ctcgaggttc gtgttgagcg cggtctcctc gatgtcttcg cccttcgtcg
     2221 agcgcgccga gttgatgtcg atggcggtca gcgcctcggt gcggtcgatc acgatgctgc
     2281 cgccggaagg cagctggacc ttgtgaccgt aggccgactc gatctggctc tcgatctgat
     2341 accgcgtgaa cagcgggacc gtgttgtcct cgtagagctt gagcttcttg atgttgtgcg
     2401 gaagcacgcg ctccatgaac tcgacggctt cctggtagat cgtcgggttg tcgatcagga
     2461 tttcgccgat gtcgttcgag aagtagtcgc gcagcgcgcg cacgatcgcg ttgccttcct
     2521 gatagatcag gaatggcgcc ggccgcgtga cgacgacctg cttgatcgct tcccagacgc
     2581 gcagcaagta gtcgaggtcc cacttgagct cctcggtcgt gcggccgatg ccggcagtac
     2641 gcacgatgca gcccatgccg tcgggcacct gcaggtcctc gagcgactgg cgaacggtgt
     2701 cgcgctcctc gccctggatc cgccgcgaaa cgccgcccgc gccggggttg ttcggcatca
     2761 gcacgatgaa ccggcccgcc aggctgatga aggtcgtcag ggcggcgcct ttggtgccgc
     2821 gctcctcttt atcgacctgg acgacgattt cctggccttc gcgcaggacg tcgcggatcc
     2881 ggacctcgcc cgaatcgggc tgcttgacga agtactcgct cgagatttcc
//
LOCUS       DASSNN010000182         7730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1046229, whole genome shotgun sequence.
ACCESSION   DASSNN010000182 DASSNN010000000
VERSION     DASSNN010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046229"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFL84_03265"
     CDS             <1..722
                     /locus_tag="VFL84_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7607671.1"
                     /translation="SFARWIADGGSAIGRSLGVSRGPSSTAVADIVGVVPDLITEVGT
                     TEPFIAYQPFAQLRRASTATLVLRAADDPGAAMREALAAARALDTRVTLLQVTTLDER
                     MAEQMSPQRFGIHVLGALGGIALLLTVLGTYVVAQSMVVRRRRELGIRAALGARREQL
                     RGLVLRDTARLVGVGLIAGLGLAFAGARLIRSLLYQVEPLDPLVLVTTAAGIFALALL
                     VSLRPAIEATRLDLTRSLREE"
     gene            complement(755..2839)
                     /locus_tag="VFL84_03270"
     CDS             complement(755..2839)
                     /locus_tag="VFL84_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET7607672.1"
                     /translation="MHPRLLASALLATACLYGCGDPGTGPAPAAAPQSAAQAEPEFVV
                     EADRFADIRVLRYRIPGWESLSLQQKKLLYYLTEAGLSGRDIMWDQNYRHNLRVRRTL
                     EEIVRRYPGDRTTADWNAFMTYVKRVWFANGIHHHYAGDKLKPGFSPEYFRTLARQSA
                     TDATWPLDPGQTLDELLAELEPILFDPAVDARKTETAAGVDKVKASAVNFYSGVTEAE
                     VLAYYAARTNSDDPRPVSWGLNSRLVRENGELVEKVWKVGGTYTQALERVVFWLEKAI
                     TVAEDDAQRRALELLVRYYRSGDLKDFDAYNIAWVEDKASRVDAINGFIEVYNDPLGM
                     RGSYESVVSFRDDEATRRIAAIASNAQWFEDNSPIMAAHRKKNVTGITGKVITVVSEA
                     GDASPVSPSGINLPNSGWIRAEHGSKSVVLSNIRAANDASPNPALEEFAASEEEIARS
                     KQWAELGGNLFVDMHEVIGHASGVVNAGIGTPTETLKQYYSPLEEGRADLVALYYLMD
                     PKLIEIGVMPSLDVGMQQYDAYFRNGLLTQLQRIEPGKNLEEAHMRNRQMISAWVFEK
                     AQPDRAVERVTRDGKTFFVVRDYQRVRELFGELLREVQRIKSEGDFAAGQALIENYGT
                     VVDPELHAEVLRRYEPLNVAPFSGYINPRLVPVTAPGSDEITDVRVEYPDDFAEQMLE
                     YAASYSFLPNVN"
     gene            complement(2961..5564)
                     /locus_tag="VFL84_03275"
     CDS             complement(2961..5564)
                     /locus_tag="VFL84_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7607673.1"
                     /translation="MGRRRAAAPTKITRGSLQESRSSKLAEQRLQSVIELTADCYWEQ
                     GVDHRFTLYRPSGAPDADLETLVGKTSWEVSPEPPEGGWEPFRALLRQRAPFRDVLHR
                     VASATKGTRYVSFSGQPVFDERKAFRGYRGVARDVSAEIWGTTLIQLENVVARVLTEA
                     DNVEDGLRRVLEAMCSSQQWTVGNFWRVDELRDALHLVVGWCTPQGEQSSLIAEGDRL
                     PPWLTGGPVWIGDVAREPSTGRLERVAGPGWNTALMVPVKAGQATVGVLEFCAPRVAA
                     PDPQFLRVLRTTSSEIAHFYQRSVAIEKLRESEERFSSTMELAAIGIAHVGNGGRFIY
                     VNPQLCAMLGYTEQELLSMTVRDVSHPGDMTLTDESGDKLRAGEIRSFKMEKRYLRKD
                     GTPVWVGLTIASKRDRDGEYRYDISVVEDISARKGAEERIQYLATHDGLTGLPNRVMF
                     GQILQLAIETARRYDRKLAVLFIDLDRFKIINDTLGHEAGDVLLREMAARLRECLRAS
                     DVVARLGGDEFVVLLQEINEPEQAATVARNILSVVMKPLVILGQECRVTASIGICMHP
                     GGGQDDHAVMKNADMAMYLAKEDGKNTFKFFSDQLKPQSVERLKLETNLRRALERDEF
                     ALHYQAKVNFKTGAITGVEALLRWQNAELGSVSPARFIPLAEETGLIVPIGKWVLRTA
                     CTQSVAWREQGLPPVSMSVNLSLRQLNDERLVPEIQAVLEETGMDPSLLELEVTESSI
                     MQNAGRAAEILTAIKRLGVRLAIDDFGTGYSSLSHLKRFPIDTLKVDRSFIREVPNDA
                     EDKAIAEAIIAMGKTLSLTVVAEGVETPQQQAFLSEKLCDEMQGYYFSTPVAAQDFAE
                     LLRSHAPKPLR"
     gene            5839..6330
                     /locus_tag="VFL84_03280"
     CDS             5839..6330
                     /locus_tag="VFL84_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET7607674.1"
                     /translation="MFVTLRLVLAAAAIAVVSSAVPQGVVTQRILSLGAARTIAEAAL
                     AECASRGFHTSVVVVDRSGDVLVVLRHELAHPVTVEMARSKAYTAVVFRGSTLDFQQQ
                     TANDPARLPQRDVPGILALAGGVPIRSAGEIIGAVASSGSSQPTDDECAKAGAAKGGS
                     LLE"
     gene            6870..7232
                     /locus_tag="VFL84_03285"
     CDS             6870..7232
                     /locus_tag="VFL84_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607675.1"
                     /translation="MSPSLVPPLVESPASAETLDFSSLVARLRKTKAINLRTKVAVRN
                     ESDELLEQFRAYHTQTGDASLAELRRAYDSLFHQLHSLLERSDPPLARDIDRSQAAIW
                     ELLADPTKFRASALMAGA"
     gene            7367..>7730
                     /locus_tag="VFL84_03290"
     CDS             7367..>7730
                     /locus_tag="VFL84_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607676.1"
                     /translation="MGTALATTTVAQVGHPAKGSWSGYWGTSDATKHRILLVLDWRDR
                     KITGVINPGPNQVPIDKADLDVDTWTLRLEASMPTSAGGKAPFVTTGKLTNLGSWTMR
                     TYSGTYTFGTETGKFTVTL"
BASE COUNT         1230 a         2570 c         2546 g         1384 t
ORIGIN      
        1 aatcgttcgc ccgctggatc gccgacggcg gcagtgccat cgggcggtcg ctgggcgtgt
       61 cccggggacc atcgagcacc gccgttgcgg acatcgtcgg cgtcgtgccg gatctgatca
      121 ccgaagtggg cacgactgag ccgttcatcg cgtatcagcc gtttgcacag ctccggcggg
      181 ccagcaccgc gacgctagtc cttcgcgccg ccgacgaccc gggagctgcg atgcgcgagg
      241 cgctggcggc ggccagagcg ctggacactc gcgtcacctt gttgcaggta acgacgctgg
      301 acgagcggat ggccgaacag atgagcccgc aacggtttgg gattcatgtc ctcggcgcgc
      361 tcggcggcat tgcactgctg ctcacggtgc tcggcacgta cgtcgtcgcg cagtcgatgg
      421 tcgtccgccg ccgccgcgag ctcggcattc gcgccgcgct cggcgcgcgc cgcgagcagc
      481 tccgtgggct cgtcctgcgc gacacggcgc ggctcgtcgg cgtcgggctg atcgcggggc
      541 tcgggctcgc gttcgcaggt gcgcgcctga ttcgttcgtt gctctatcaa gtcgagccgc
      601 tcgatccgct cgtgctcgtg acgacggcgg ccggcatctt cgcgctcgcg ctgctcgtga
      661 gcctgcgccc cgcaatcgaa gcgacgcggc tcgatctgac tcggtcgttg cgcgaagagt
      721 aaccgccgcg gcgctttgcg gcgtggcgtc ttggtcagtt gacgttcggc aggaacgaat
      781 agctcgccgc gtactcgagc atctgctccg cgaaatcgtc gggatactcg acgcgaacgt
      841 ccgtgatctc gtcgctgccg ggagccgtga ccggcacgag acgcggattg atgtagcccg
      901 agaacggcgc gacgttgagc ggctcgtaac gccgcagcac ctctgcgtgc agctccgggt
      961 cgaccaccgt gccgtaattc tcgatcagcg cctgccccgc ggcgaagtcg ccttcgctct
     1021 tgatcctctg cacctcgcgc aacagctcgc cgaagagctc gcgcacgcgc tggtagtcgc
     1081 gcacgacgaa aaaggtcttg ccgtcgcgcg tgacgcgctc gacggcgcgg tcgggttgcg
     1141 ccttctcgaa cacccaggcc gagatcatct gccggttgcg catgtgcgct tcctcgaggt
     1201 tcttgccggg ctcgatgcgc tggagctgcg tcagcagacc gttgcggaag taggcgtcgt
     1261 actgctgcat gccgacgtcg aggctcggca tgactccgat ctcgatgagc ttcgggtcca
     1321 tgaggtagta aagcgccacg aggtcggcgc ggccttcctc gagcgggctg tagtattgct
     1381 tgagcgtctc ggtcggagtg ccgatgccgg cattgactac gccggacgcg tggccgatga
     1441 cctcatgcat gtcgacgaac aagttgccgc cgagctcggc ccactgcttc gaacgcgcga
     1501 tctcctcctc ggatgccgca aattcctcga gcgccgggtt cggcgaggca tcgttcgcgg
     1561 cgcgaatgtt cgacagcacg accgacttgc tgccgtgctc cgcgcgaatc cagcccgagt
     1621 tcggcaggtt gatgccgctc ggcgaaaccg gcgacgcgtc gccggcctcg gagacgaccg
     1681 tgatcacctt gcccgtgatg cccgtgacgt tcttcttccg gtgcgcggcc atgatcggcg
     1741 agttgtcctc gaaccactgc gcattgctcg cgatcgcggc aatgcgccgt gtggcctcat
     1801 cgtcgcggaa tgaaacgacg gactcgtacg agccgcgcat gccgagcgga tcgttgtaga
     1861 cctcgatgaa gccgttgatc gcgtcgacgc gcgaggcctt gtcctcgacc cacgcgatgt
     1921 tgtacgcgtc gaagtccttc agatcgccgc tgcggtagta ccgaacgagc agctcgagcg
     1981 cgcggcgctg cgcgtcgtcc tcggctaccg tgatcgcctt ctcgagccag aagacgacgc
     2041 gttcgagcgc ctgcgtgtaa gtgccgccga ccttccacac cttctcgacg agctcgccgt
     2101 tttcgcgaac gagccgcgag ttcaagcccc agctcacggg gcgcggatcg tctgagttgg
     2161 tccgcgcggc gtagtacgcg agcacctcgg cttccgtgac tcccgaatag aagttgactg
     2221 ccgaagcctt caccttatcg acgccggccg cggtctcggt cttgcgcgcg tcgacggcgg
     2281 gatcgaacag gatcggctcg agctcggcca agagctcgtc gagagtttgg ccgggatcga
     2341 gcggccacgt cgcgtctgtc gcgctctgcc gcgccagcgt gcggaaatat tcgggcgaaa
     2401 aacctggctt gagcttgtcg cctgcgtagt gatgatggat cccgttcgca aaccagacgc
     2461 gcttcacgta ggtcatgaac gcgttccagt cggcggtcgt gcggtcgccg ggatagcggc
     2521 gcacgatctc ctcgagcgtg cggcgcacgc gcaggttatg gcggtagttc tggtcccaca
     2581 tgatgtcgcg gccgctcaga cccgcttccg tcaagtagta cagaagcttt ttctgctgca
     2641 gcgagaggct ttcccagccg ggaatccggt agcgcagcac gcggatgtcg gcgaagcggt
     2701 cggcctcgac gacgaactcg ggctcggctt gcgcagcgct ttgcggagcc gcggccggtg
     2761 cgggccccgt gccgggatcg ccgcagccgt acaaacaagc cgtggcgaga agcgccgatg
     2821 ccaagagacg cggatgcatg ggatgctcct gccgcgagcg gtcgaacctc cgagatggca
     2881 cgagtctagc aaagcgaagc gagcgacgtc gtcggcacga ccgtgcgaca gaagatcgag
     2941 aagctcccgc ggcgccgagc tcagcgcagc ggcttcggcg cgtggctgcg caggagctcg
     3001 gcgaagtctt gcgcggcgac cggcgtgctg aagtagtagc cctgcatctc gtcgcagagc
     3061 ttctcgctca agaacgcttg ctgctgcggc gtctcgacgc cttcggcgac gaccgtgagg
     3121 ctcaaggtct tgcccatcgc gatgatcgct tccgcgatgg ctttgtcctc ggcgtcgttc
     3181 ggtacttcgc ggatgaacga gcggtccacc ttcagcgtgt cgatcgggaa acgcttcagg
     3241 tgcgacagcg acgaatagcc tgtgccgaag tcgtcgatcg cgagccgcac gccgagccgc
     3301 ttgatggcgg tcaggatctc ggcggcgcgc cccgcgtttt gcatgatcga gctctccgtg
     3361 acctcgagct cgagcagtga agggtccatg ccggtctcct cgagcacggc ctgaatctcg
     3421 ggcacgaggc gttcgtcgtt gagctgccgc aacgacaggt tgacggacat gctgacgggc
     3481 ggcaggcctt gctcgcgcca cgcgacgctc tgcgtgcacg ccgtgcgcag cacccacttg
     3541 ccgatcggca cgatgagccc cgtttcctcg gccagcggga tgaagcgggc cggcgagacg
     3601 gagccgagct cggcgttttg ccagcgcagc aatgcctcga cgcccgtgat ggcaccggtc
     3661 ttgaagttga ccttcgcctg atagtgcagc gcgaattcgt ctcgttcgag tgcgcggcgt
     3721 aagttggttt cgagcttcag gcgctcgacg gactgcggct tcaactgatc gctgaagaat
     3781 ttgaaggtgt tcttgccgtc ctccttcgcg agatacatcg ccatgtcggc gttcttcatg
     3841 acagcgtggt cgtcttgccc gcccccgggg tgcatgcaaa tgccgatgct cgccgtcacg
     3901 cggcactctt ggcccagaat cacgagcggc ttcatgacga ctgacagaat gttgcgcgcg
     3961 acggtcgcgg cttgctccgg ctcgttgatc tcctggagca gcacgacgaa ctcgtcgccg
     4021 ccgagccggg ccacgacgtc gctggctcgc aggcactcgc gcaagcgcgc ggccatctcg
     4081 cgcagcagca cgtcgccggc ttcgtgtccg agcgtgtcgt tgatgatctt gaaacggtcg
     4141 agatcgatga acagcacggc gagcttgcgg tcgtagcggc gcgccgtctc gatcgcaagc
     4201 tgcaagattt ggccgaacat gacgcggttc ggcaggcccg tgagcccgtc gtgcgtcgcg
     4261 agatactgga tgcgctcctc ggcgcctttg cgtgccgaga tatcctcgac gaccgagatg
     4321 tcgtaacggt actcgccgtc acggtcgcgt ttcgacgcaa tcgtaaggcc cacccagaca
     4381 ggcgtcccgt ccttgcgcag ataccgcttt tccatcttga acgagcgaat ctcgcccgcg
     4441 cgcagcttgt ctcccgattc gtcggtcaac gtcatgtcgc cggggtgaga aacgtcgcgc
     4501 acggtcatcg acagcagctc ttgttccgtg tagccgagca tcgcgcagag ctgcgggttc
     4561 acgtagatga aacgcccgcc gttgccgaca tgtgcgatgc cgatcgcggc gagctccatg
     4621 gtcgaggaga accgctcctc gctctcgcgc agcttctcga tcgccacgga gcgctgatag
     4681 aaatgcgcga tctccgagct cgtcgtgcgc agcacgcgca ggaactgcgg gtcgggcgcg
     4741 gcgacgcgcg gcgcgcagaa ctcgagcacg ccgacagtcg cttggccggc cttcaccggc
     4801 accatcagcg ccgtgttcca cccaggaccc gcgacgcgtt cgagccggcc cgtgctcggc
     4861 tcgcgagcca cgtcgccgat ccagaccggt ccgcccgtca gccacggcgg cagccgatcc
     4921 ccctcggcga tcagcgagga ctgctcgccc tgcggcgtac accatcccac gacgagatgc
     4981 agcgcatcgc gcagctcgtc gacgcgccaa aaattgccga cggtccactg ctgtgacgag
     5041 cacatcgcct cgagcacgcg ccgtaaaccg tcttcgacgt tgtccgcttc ggtgagcacg
     5101 cgcgcgacca cgttctcgag ctgaatcagc gttgtacccc atatctcggc gctcacgtcg
     5161 cgcgcaacgc cgcggtagcc acggaaggcc ttgcgctcgt cgaagaccgg ctgcccgctg
     5221 aagctcacgt accgagtgcc cttcgtcgcg cttgccacgc ggtgcagcac atcgcgaaac
     5281 ggcgcgcgct ggcggagcag cgcccgaaac ggctcccagc cgccttcggg cggctcgggc
     5341 gaaacctccc agctcgtctt gccgaccagc gtctcgagat cggcgtccgg cgcgccgctc
     5401 gggcggtaaa gcgtgaagcg gtggtctacg ccttgttccc agtagcagtc cgcggtgagc
     5461 tcgatcaccg actgcaatcg ctgctcagcc aatttggacg accggctttc ctgcaaggag
     5521 ccgcgcgtga tcttcgtcgg cgcggccgcg cgtctgcggc ccatcagagc ctccatgatc
     5581 cccgttgcat cgcggtgccg gggcgaggag tccattccgt gggcctgctg tttggcgggg
     5641 cccgctggcg acggtggcgc gaagttcctc aagaatagcc gcaacgcgac gcggctaatg
     5701 cgtaacggct ctcattcgac ggggggcggg gttgatactg gtcacagagc cgcgaggcgc
     5761 gccgaagccg tccgaatttg tgctggcagc cgcacgcgcg tgtggtatct actatcgcgg
     5821 gctcccggag gcatcgccat gttcgttacg ttgcgtttgg tgctcgcggc ggcggcaatc
     5881 gccgtcgtat ccagcgcggt gccgcagggt gtcgtcacgc agcgtatttt gtcgctcggc
     5941 gcggctcgca cgatcgccga agccgcgctc gcggaatgcg cgtcgcgtgg gttccacacg
     6001 agcgtcgtcg tcgtcgatcg gtccggcgat gtgctcgtcg tgctgcgtca cgagctcgct
     6061 catcccgtca cggtggaaat ggcgcgcagc aaggcataca cggccgtcgt cttccgcggc
     6121 tcgacgctcg atttccagca gcagacggcg aacgacccgg cgcgcctgcc gcaacgcgac
     6181 gtgcccggca tcctcgccct cgccggcggc gtgccgattc gctcggccgg cgaaatcatc
     6241 ggggccgttg cgagctcggg atcgagccag ccgaccgacg acgagtgcgc gaaggcgggt
     6301 gcggcgaaag gcgggagcct gctcgagtag cgcgcaagtt tcccctgcga ccgaatttcc
     6361 cgttcggacg cgtcaaactt ccgggaaggc agccctgccg ttcgcgatac gggttcctga
     6421 cgattcagtt tgcgttcagc gcccacatgc ggatatagga cccaagggcg gttcggcgcg
     6481 gttcgggact cacggatcgg agatggcaaa tgattcgcgg tgtgagctta cggcaagggc
     6541 ttatttgcgt gtggtgcctc ggcgcggtgg gctgtgcgga agtaacgagc gagtcgcctc
     6601 acgacgcgac cggagctgcc gagactgccg cggtcgacac agcacagtct agcgacgtcg
     6661 cgctgccgtc ggagcccgcg tcacccgccg cggtcgcaga cgtcagagac accaccgcag
     6721 atcacgccat cgcggatccg atcgatgctg cgcctctgtc gaccggatcg gaccctgagc
     6781 tgccgacggg ctccaccgga gacatcgccc tcgaacgagc atccgagccg gtcgccgcgc
     6841 ctgaagcttc gaccgttcgg gaattcgcag tgtcgccgag cctcgttccg cccttggtcg
     6901 agtcgccggc ctccgcggag acgctcgact tctcgtcgct cgtcgcgcgg ctgcgaaaga
     6961 cgaaggcaat caacttgcgg acgaaggtcg ccgtcaggaa cgaatcggac gagctgctgg
     7021 agcagttccg cgcgtatcac acgcagacgg gagacgcgtc gcttgccgag ctgcggcgcg
     7081 cctacgactc gctatttcac cagttgcact cgctgctcga gcgctccgat ccgccgctcg
     7141 cgcgtgacat cgaccggtcg caggctgcga tctgggagct cctggcagac ccgaccaagt
     7201 tccgcgcatc cgctttgatg gccggagcct gacgtcaggc gatcggctcg ccgccgcgct
     7261 gcgcgcgccg tgacgagtac cgtcaatgct atattgacgc tcgagatcct tgctcgcgca
     7321 cggcgcgccg ctacgcatga cacgatccgc ttttctcgga ttgctattgg gtactgccct
     7381 cgccacgacc acggtcgcgc aggtcggcca cccggcgaag ggttcctgga gcggctactg
     7441 gggcacgagc gatgccacca agcaccgcat tctgctcgtg ctcgactggc gcgatcggaa
     7501 gatcaccggc gtcatcaatc ccgggcccaa ccaagtgccg atcgacaagg ccgacttgga
     7561 cgtcgacacg tggacgttac ggctcgaggc ttcgatgccg acgagcgccg gcggcaaagc
     7621 gccgttcgtc acgaccggca agctcaccaa tctcggctcg tggacgatgc ggacctattc
     7681 gggcacgtac acgttcggga ccgagaccgg caagttcacc gtgacattga
//
LOCUS       DASSNN010000183         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_416282, whole genome shotgun sequence.
ACCESSION   DASSNN010000183 DASSNN010000000
VERSION     DASSNN010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416282"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1811)
                     /locus_tag="VFL84_03295"
     CDS             complement(<1..1811)
                     /locus_tag="VFL84_03295"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET7607677.1"
                     /translation="MNVTPRDEAQLLNRTVLASTISALLAGGAATQAQAQDQALEEIT
                     VTGSRIVRRDLEAASPIMTVDTQALENSSTMSIESVLNQMPQFVPDNTQFDGGTNVAS
                     GAVTPGIASVNLRGIGANRTLVLIDGRRAQPVNASLIVDLNTIPSAAIERVETITGGA
                     SAVYGADALAGVVNFVLKDDFEGVEMDFSSSMTAEGDGNETRFTSLLGVNGEGGNGNV
                     MLGVEWYKRELVLQRDREFYRHGWEDSTNQTGGFLNATGFSPGQVTVNTSNITVNQSV
                     LPLNRPTQAAVDAVFAPYGIAPGTVSNSTEIYFQPDGRPFTLTGVNYTGPIMSYDLNG
                     DGLAGVHRQPNGSLQQVNIDGLLATPLERRSLFGRATFDMNDNLTAYAQATYSNIETT
                     TRGGFPPAITVWQAPVPNDGLRPLPPNLQQLLDSRTYDPDGPTGPLPVQSGAALPWNL
                     FRGIDFLGGPQAPTTETDAYQLMVGIEGALSNRDWTYDAYVSTGETDILIFYDDLPSL
                     QRYQFLVAQPNWGQGSFVRGRNYDVTCGTGLPMFTLASQAPDAGCQEAMQSKLRPIQK
                     LSQNIMEANLQGKIADMSNGELRFAAGISNRQNKFRY"
     gene            2123..>2514
                     /locus_tag="VFL84_03300"
     CDS             2123..>2514
                     /locus_tag="VFL84_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET7607678.1"
                     /translation="MPTFRRALILTHRYLGIPLSVLFVLWFVTGIAMIYVGGMPTLSA
                     QTRLAHLPPLDLAAVRYTPAEAAERAASGFGRVALTTVLGRPAYRFASAYGSATVFAD
                     TGEPLDEIDVDTARGVAAEFVGAKVSDV"
BASE COUNT          418 a          809 c          808 g          479 t
ORIGIN      
        1 tcgtagcgga atttgttctg gcgattgctg ataccggccg cgaacctgag ctcgccgttg
       61 ctcatgtcgg cgatcttgcc ttgcaaattc gcctccatga tgttctgcga gagcttctgg
      121 atcggccgca gcttcgactg catggcttct tggcagcccg cgtcgggcgc ctgcgaggcc
      181 aacgtgaaca tcggcaggcc cgtgccgcag gtgacatcgt agttgcggcc gcgcacgaac
      241 gagccttggc cccagttcgg ctgcgcgacg aggaactgat aacgctgcag cgacggcaga
      301 tcgtcataaa aaatgagaat gtccgtctcg ccggtcgaga cgtaggcgtc atacgtccaa
      361 tcgcggttcg acaacgcgcc ctcgatgccg accatgagct gatacgcgtc ggtctcggtc
      421 gtcggtgctt gcggaccgcc gaggaagtcg atgccgcgga agaggttcca tggcaaggcc
      481 gcccccgact gcaccggcag cggtcctgtc gggccgtcgg ggtcgtaggt gcgcgaatcg
      541 agcaactgct gcaggttcgg gggcaatggc cgcaagccgt cgttgggcac cggcgcttgc
      601 caaacggtga tcgcgggcgg gaaaccgccg cgcgtcgtcg tctcgatatt cgagtaagtc
      661 gcctgtgcat acgccgtcag gttgtcgttc atgtcgaacg tcgcgcgccc gaacagcgag
      721 cgacgctcga gcggcgtcgc gagcaacccg tcgatgttga cctgctgcaa gcttccgttc
      781 ggttgacggt gcacaccggc caagccgtcc ccgttcaggt cgtagctcat gatcgggccc
      841 gtgtagttga cgccggtcaa cgtgaacggc cgtccatcgg gctgaaagta gatctctgtc
      901 gagttgctga ccgtaccggg tgctatgccg tacggcgcga acaccgcgtc gacggccgct
      961 tgcgtcggcc ggttcagcgg cagcacggac tggttcaccg tgatgttgct cgtgttgacc
     1021 gtgacctggc ccggcgagaa tcccgtcgcg ttcaagaagc cgccggtctg gttcgtcgag
     1081 tcttcccagc cgtggcgata gaactcgcgg tcgcgctgga gcaccagctc gcgcttgtac
     1141 cactcgacgc cgagcatgac gttgccgttg ccgccctcgc cgttgacgcc gagcaggctc
     1201 gtgaagcgag tttcgttgcc gtcgccttcg gcggtcatcg acgacgagaa gtccatctcg
     1261 acgccttcga agtcgtcctt cagcacgaag ttgacgacgc ccgcgagcgc gtcggcgccg
     1321 tacacggccg aggcgccgcc cgtgatcgtc tcgacgcgct cgatagcggc cgacggaatc
     1381 gtgttcaagt cgacgatcag cgaagcgttg acgggctgcg cgcggcggcc gtcgatcagc
     1441 acgagagtgc ggttcgcgcc gatgccgcgc aggttgacgc tggcgatacc gggcgtcacc
     1501 gcgccggacg cgacgttggt gccgccgtcg aactgcgtgt tgtcgggcac gaactgcggc
     1561 atctggttca acacgctttc gatactcatc gtgctcgagt tctcgagcgc ctgcgtgtcg
     1621 accgtcatga tggggctcgc ggcctcgagg tcgcggcgta cgatgcgaga tcccgttacg
     1681 gtgatctctt cgagcgcctg gtcttgcgcc tgcgcctgcg tggctgctcc gcccgcgagc
     1741 agtgccgaga tcgtcgacgc cagcacagtg cgattcagca actgtgcttc gtcacgtggt
     1801 gtgacattca ttgctaaccc cccgaggttc gttgtaaaag caaatcattc cgaaaacgat
     1861 agaactcggc cgcacttccg gtgttgccaa aaaacttcgg ccgacacgac gacttgacag
     1921 aaaagtgccg ctgccgccgg actttgcatc actccgggcg ctttatgcca ggctatatcc
     1981 ataactatca tcattgctac cagttgtacg cgcacaacga cgaggaattc caaggctttc
     2041 aagcgaaccg ctcggtccac caccgactcg tcgcgcgcgg ccggcggccg ctacactgct
     2101 aaccgcctcg agagagccaa cgatgccgac tttccgccgc gcactgatcc tgacccaccg
     2161 ctatctcggc attccgctga gcgtcctgtt cgtgctctgg ttcgtgaccg gaatcgcgat
     2221 gatctacgtc ggcggtatgc cgacgctgag cgcgcagaca cggctcgcgc atctgcctcc
     2281 gctcgacctc gcggccgtgc gctatacgcc ggccgaggcg gccgaacgag cggcgagcgg
     2341 cttcggccgc gttgcgttga cgacggtgct cggccgtccc gcgtatcggt tcgcgagcgc
     2401 gtacggctcg gcgacggtgt tcgccgacac gggcgagccg ctcgacgaga tcgacgtcga
     2461 cactgcgcgc ggcgtcgccg cggaattcgt cggagcgaag gtcagcgacg tcga
//
LOCUS       DASSNN010000184         4773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1551417, whole genome shotgun sequence.
ACCESSION   DASSNN010000184 DASSNN010000000
VERSION     DASSNN010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551417"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="VFL84_03305"
     CDS             complement(<1..1396)
                     /locus_tag="VFL84_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7607679.1"
                     /translation="MKPHRHKVVLYNPRAVFYTMPLALLAIGSELDPDKYEVVIVDGR
                     LDPNAERSVLAHLDGALCLGVTVLTGAPIADALRVSRAAKAARPGVPVVWGGWHPSMF
                     GRQCLDETCVDVTVQAQGEATFAEIVQRLVAGTSLEGCNGCMFRRPDGRVHVNPPREL
                     LPLDRFRPHDYSLIPVERYYELKGKRQLDYISSQGCNFRCAFCSDPFVYGRKWTGIEP
                     GSMVAKLLELWRRYRFDDVNFQDETFFTKADRVEAMARGIVDSGVRFTWAATMRADQG
                     VRLPHEVWKLCKQSGLRRLLVGVESGDDEMLRRIKKDIKIEKVFRTAERMRALGIAGI
                     FPFIVGFPDESDASVDVTLACARKLREMSGAFETPIFYFKPYPGSAIVADAVAKGFEL
                     PSTLTDWSQFDYVAGLPGPWVPPEKYRLIERFKFFLDLGSKRGGWATSWLRPLARLRC
                     ERREFRWPVEMAVMK"
     gene            complement(1393..2124)
                     /locus_tag="VFL84_03310"
     CDS             complement(1393..2124)
                     /locus_tag="VFL84_03310"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7607680.1"
                     /translation="MLDASLPSLLDDDRPGALGILAGRVLRLAYTLSGKHRYDDFRLE
                     RVLGIPIMVIPGVANPKLLRTGAFFASRIDARLIGAGASVLDMGTGSGVCALFAARHA
                     RRVVAVDISAAAVRCTRLNAALNHLEERIDVRHGDLFAPVARERFDLVLFNPPFLLGA
                     PKDDRDATWRSSDAARRFAAGLGDPLEPAGAALVLLSSFGDACALFESELRANGFRLE
                     VFARRRFINETLTILRATRAAVPRA"
     gene            complement(2117..3124)
                     /locus_tag="VFL84_03315"
     CDS             complement(2117..3124)
                     /locus_tag="VFL84_03315"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7607681.1"
                     /translation="MADALPLVTLYLSERCNSRCVTCDYWRHGEKDMTLESVRRMLPS
                     LERLRTRAVLLSGGEPLLNPEWPEIAQALKSRGLALWLLTSGLSLLKHAARAAPLFDS
                     ITVSLDGARASTYAAIRGVDAFDKVCEGIRAVAALGVATGVRVTLQRGNYRELLDFVA
                     LARGLGAAQVSFLAVDVSNPHAFARRDDAPPELALAAGDIPVLDALLDELLAKHSADF
                     DTRFIAESPAKLRRIRDYFAALCGLGPFPSVRCNAPEFSAVVGVDGRVAPCFFIPAAT
                     LAPRAPDLAASFASGPARALRTAIRAGRRRECARCVCSMWREPAALASEAFLLRAAAD
                     A"
     gene            complement(3117..3950)
                     /locus_tag="VFL84_03320"
     CDS             complement(3117..3950)
                     /locus_tag="VFL84_03320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7607682.1"
                     /translation="MTADNEAMTPQGRSVEAPAFDDLAAAYDAAFTASALGRSLRALT
                     WERLDAALGSSRRVLEIGCGTGEDARRLALRGVDVLATDPSPSMLRLAAEKAAKAGCA
                     KRIEFRCVPMERLGAELAGERFDGVWSNFGAINCIPKLGAVVAELAALLAPGAPLAWV
                     VMGRHVPWEWAWFLSRGNPRKAFRRQRRGGAVWRGMRIVYPTPRELTRTLAPHFTPTR
                     CRPLGCVLPPSYASGWLERRPRVLGALARVERAAQRFAPLAALADHYIFEARLSRARD
                     G"
     gene            4096..>4773
                     /locus_tag="VFL84_03325"
     CDS             4096..>4773
                     /locus_tag="VFL84_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607683.1"
                     /translation="MPFVSHLRSAAAAAVLGALLASAPAAAQWVGYPTAHVPRKADGK
                     VDMTAPAPRLANGKPDFSGIWISDRTEPGQETISDTSSLPSGRHMQDMGVDMEGGLPY
                     QPWQLPIVKRRTENLAIEDPHIRCLPDFFLRAYGLPHMLKFVHTPDLLVMLNEMNAGY
                     RQVFTDARALPDTPTPSWQGYSSAKWDGDTLVVDTIGMRDDTWIDWHGSVVTEAAKIR
                     EEIRRLDF"
BASE COUNT          794 a         1645 c         1687 g          647 t
ORIGIN      
        1 gcttcatgac ggccatctcg accggccagc ggaattccct gcgctcgcac cgcagccgcg
       61 cgagcggcct gagccaagac gtcgcccagc cgccgcgttt cgagccgaga tcgaggaaaa
      121 atttgaagcg ctcgatgagc cggtattttt ccggcgggac ccacggcccc ggcaggcccg
      181 cgacgtagtc gaactgcgac cagtcggtca gcgtgctcgg tagctcgaat cccttcgcga
      241 cggcgtccgc gacgatcgcg ctgccggggt agggcttgaa gtagaagatc ggcgtttcga
      301 acgcgccgct catctcgcgc agcttgcgcg cgcatgcgag cgtgacgtcg acgctcgcgt
      361 cgctctcgtc cgggaagccc acgatgaacg gaaagattcc cgcgatcccg agcgcgcgca
      421 tccgctcggc cgtgcgaaag actttttcga tcttgatgtc cttcttgatg cgtctcagca
      481 tctcgtcgtc gcccgactcg acgccgacga gcaggcgccg cagccccgat tgcttgcaga
      541 gcttccagac ctcgtgcggc agccggacgc cctggtcggc gcgcatcgtc gcggcccagg
      601 tgaagcggac gccggaatcg acgatgccgc gcgccatcgc ctcgacgcga tcggccttcg
      661 tgaagaacgt ctcgtcttgg aagttgacgt cgtcgaagcg gtaccggcgc cagagctcga
      721 gcagctttgc gaccatcgag ccgggctcga tgccggtcca cttgcggccg tacacgaacg
      781 gatcggagca gaacgcgcag cggaaattgc agccttgcga ggagatgtag tcgagctgcc
      841 gcttgccctt cagctcgtag taccgctcga cgggaatcaa cgagtagtcg tgcgggcgaa
      901 accgatcgag cggcagcagc tcgcgcggcg gattgacgtg cacgcggccg tcgggccggc
      961 ggaacatgca gccgttgcag ccttcgagcg aggtgccggc gacgaggcgc tgcacgatct
     1021 cggcgaacgt cgcctcgccc tgtgcttgca cggtgacgtc gacgcaagtt tcgtcgaggc
     1081 attgccggcc gaacatcgac gggtgccatc cgccccagac gacgggaacg ccggggcgtg
     1141 cggctttcgc cgcgcgcgac acgcgcagcg cgtccgcgat cggcgcgccg gtcagcaccg
     1201 tgacgccgag gcagagcgct ccgtcgagat gggccaagac cgatcgctcg gcgttcgggt
     1261 cgagccggcc gtcgacgatc acgacttcgt acttgtcggg atcgagctcg gagccgatcg
     1321 cgagcagcgc gagcggcatc gtgtagaaca ccgcgcgagg gttgtacagc acgactttgt
     1381 ggcgatgcgg cttcatgcgc gcggcaccgc ggcgcgcgtc gcgcgcagga tcgtcagcgt
     1441 ctcgttgatg aaccggcgtc gcgcgaagac ctccaagcga aagccgttcg cgcgcagctc
     1501 cgattcgaac agcgcgcacg cgtcgccgaa cgaggacagc agcaccaacg ccgcgccggc
     1561 gggctcgaga gggtcgccga gcccggccgc gaagcggcgc gcggcatcgc tcgaccgcca
     1621 ggtcgcgtcg cgatcgtctt tcggagcgcc gagcaagaac ggaggattga acagcacgag
     1681 gtcgaaccgc tcgcgcgcga ctggcgcgaa caggtcgcca tggcgaacgt cgatgcgctc
     1741 ttcgaggtgg ttcagcgcgg cgttcaggcg agtgcaacgc acggccgcgg cgctgatgtc
     1801 gacggcgacg acgcgccgcg cgtggcgggc cgcgaagagc gcgcagacgc cggagcccgt
     1861 gcccatgtcg agcacgctcg cgccggctcc gatcaaccgt gcgtcgatgc gcgacgcgaa
     1921 gaaagcgccg gtgcgcagca gcttcggatt cgcgacgccg ggaatcacca tgatcggaat
     1981 gccgagcacg cgctcgaggc ggaaatcgtc gtagcgatgc ttgccgctca gcgtgtacgc
     2041 gaggcgcagc acgcggccgg ccaggatgcc gagcgcgccg ggccgatcgt cgtcgagcag
     2101 tgacgggagc gaagcgtcaa gcatcggcgg cggcccttag aaggaacgct tcgctcgcga
     2161 gcgccgccgg ctcgcgccac atggagcaga cgcaccgcgc gcattcgcgc cgtcggccgg
     2221 cgcggatcgc ggtccgcagc gcccgggcgg gcccgctcgc gaagctcgcc gcgagatccg
     2281 gcgcgcgcgg cgcgagcgtc gcggcgggga tgaaaaagca cggtgcgacg cgcccgtcga
     2341 cgccgacaac ggcggaaaac tcgggcgcgt tgcagcgaac cgaagggaag gggccgaggc
     2401 cacagagagc cgcgaagtag tcgcgaatgc gccgcagctt ggccggcgac tcggcaatga
     2461 aacgcgtgtc gaagtcggcg gagtgcttgg cgaggagctc gtcgagcagc gcatcgagca
     2521 cgggaatgtc gccggccgcc aacgcgagct cgggcggcgc gtcgtcgcgg cgggcgaacg
     2581 cgtgcgggtt gctcacgtcc acggcgagga acgagacttg cgcggcgccg agcccgcgcg
     2641 cgagcgcgac gaagtcgagg agctcgcgat agttgccgcg ctgcagcgtg acgcgcacgc
     2701 ccgtcgcgac gcctaaggcc gcgacggcgc ggatgccctc gcaaaccttg tcgaaggcgt
     2761 cgactccgcg gatggccgcg taggtgctcg cgcgcgcgcc gtcgagcgac accgtgatcg
     2821 aatcgaacag cggggccgcg cgcgcggcgt gcttcaagag cgacaagccc gaggtcaaga
     2881 gccacagcgc gagcccgcgg ctcttcagcg cctgcgcgat ctcgggccac tccgggttca
     2941 gcagcggctc gccgcccgac agcagcacgg cgcgcgtgcg cagccgctcg agcgacggca
     3001 gcatgcgccg caccgattcg agcgtcatgt ccttctcgcc gtgccgccaa tagtcgcaag
     3061 tcacgcagcg cgagttgcag cgctcgctca aatacagcgt cacgagcggc agggcgtcag
     3121 ccatcgcgcg ccctcgagag ccgggcctcg aagatgtagt ggtccgcgag cgcggccaaa
     3181 ggcgcaaagc gctgcgccgc gcgctcgacg cgcgcgagcg cgccgagaac gcgcggccgc
     3241 cgttcgagcc agcccgaggc gtagctcggc ggcagcacgc agccgagcgg ccggcagcgc
     3301 gtcggcgtga agtggggcgc gagcgtgcgc gtgagctcgc gcggcgtcgg atagacgatg
     3361 cgcatgccgc gccagaccgc gccgccgcgt cgctgccggc ggaacgcctt gcgcgggttg
     3421 ccgcgcgaca ggaaccacgc ccactcccac ggaacgtgcc tgcccatgac gacccacgcg
     3481 agcggcgcgc cgggcgcgag cagcgccgcg agctccgcga cgacggcccc gagcttaggg
     3541 atgcaattga tcgcgccgaa gttcgaccag acgccgtcga accgctcgcc ggcgagctcc
     3601 gctccgagcc gctccatcgg cacgcagcga aactcgatgc gcttcgcgca ccccgctttg
     3661 gcggcctttt cggcggcgag ccgcagcatc gacggcgacg gatccgtcgc gagcacgtcg
     3721 acgccgcgca gcgcgaggcg gcgagcgtcc tcgccggtgc cgcagccgat ctcgagcacg
     3781 cgccgcgacg agccgagcgc ggcatcgagg cgctcccacg tcaacgcgcg caggctccgg
     3841 ccgagcgcgg aagcggtgaa cgccgcgtca taggccgccg ccaagtcgtc gaacgccggc
     3901 gcctcgacgc tccggccttg cggcgtcatc gcttcgttgt cagccgtcat ttaagtcgca
     3961 gcgccgcctc tggtccgtca ccgccagccc tgtacgtccc cggcgtcggg cggatggttc
     4021 taacgggcgc gtgcgccggg agctttagtg tgggaaattt ggctcccgat cgattggccc
     4081 cgagtggagc acagcatgcc gtttgtctcg caccttcggt ccgccgccgc cgccgccgtg
     4141 ctgggcgcgc tgctcgcttc ggcgccggca gcggcgcaat gggtcggcta tccgacggct
     4201 cacgtgccgc gcaaggccga cggcaaggtc gacatgacgg ccccggcgcc gcgcctcgcg
     4261 aacggcaagc cggacttttc cggcatctgg atcagcgatc ggaccgagcc cggccaggag
     4321 acgatcagcg acacgagctc gttgccttcg ggccgccaca tgcaggacat gggcgtcgac
     4381 atggaaggag gcttgccgta ccaaccgtgg cagctgccga tcgtcaagcg gcgcaccgag
     4441 aatctcgcga tcgaggatcc gcacatccgc tgcttgccgg acttcttcct gcgcgcgtac
     4501 ggcttgccgc acatgctgaa gttcgtccac acgccggacc tgctcgtgat gctgaacgag
     4561 atgaacgcgg gctaccgcca ggtgttcacg gacgcgcgcg ctttgcccga cacgccgacg
     4621 ccttcgtggc aaggatattc gtcggcgaaa tgggacggcg acacgctcgt tgtcgacacg
     4681 atcggcatgc gcgacgacac gtggatcgac tggcacggca gcgtggtgac cgaagccgcc
     4741 aagatccgcg aagaaatacg acggctcgac ttc
//
LOCUS       DASSNN010000185         3074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_776055, whole genome shotgun sequence.
ACCESSION   DASSNN010000185 DASSNN010000000
VERSION     DASSNN010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776055"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..636
                     /locus_tag="VFL84_03330"
     CDS             31..636
                     /locus_tag="VFL84_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607684.1"
                     /translation="MNQIEALRRLRRLGAPVFETRDASALLEVTPANANMILRRLADQ
                     DLIVHLARGRWAMGTSVPRFALPELISAPYPAYVSMQSALFHRGLIEQVPAVIYAVTL
                     GKPRRVSTPLGTISFHRLPPELFRGYELEPDGSKIATAEKALFDTLYLAPARSRLFAR
                     LPELEIPREFRWQELHELAARVKFERRRVHIERRIEQLERR"
     gene            686..>3074
                     /locus_tag="VFL84_03335"
     CDS             686..>3074
                     /locus_tag="VFL84_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607685.1"
                     /translation="MLLDLKSACRELLKNRWFTCVTVLTLALGLGANTAIFGVVNKLL
                     LNPLPYPDSDRIVYLRVGLQRAQFVFPPPVAVASAWREEARSFEGFEGSSSNSVLAYD
                     ENGARLLRGMRFTPGLPAFLGVAPVLGRGFTPADIEPGAPAVVMLSYEMWQRDYGGSR
                     DVVGRAITLDDLPHVVVGVMPPRWDAFAVGFRPEVLFPQAYPVAGAQPVSVDIMTRLR
                     EDVSLDAATNELAAILARVSAESPRPLFGPDAPTVKIQTPTERTAANTRDALLVLLAA
                     VGLVLLVACSNVANLLLARGASRSRELSLRSALGASTWRLVRALFAECLVLALAAGVV
                     GLGVGWLTLRILVGLRPGSLTAIGEAELDPTVLAFTFGVSVLTALLFGLAPALQLASR
                     RLGDALRGGASGVVRGGMGPRLRKLLVAAQMAISVVLLVSAGLLIRSFIHLQSAEIGF
                     DAENLFSVQLSMPRAKYQTPTSREVFAEQLLERLRSSPGVTTATQVFSAPPNGVTFVG
                     SSGFEIRGASLSEADAQAARIMHFAGADYFSALRIALIEGRTFTADEMRSKTAVIVNR
                     AAAEHFWPDGEALGGEIKWQQDWATVIGVVDNVLFGSLTRGRDTPQFYWPLPMGPSFT
                     GGPASLTFVVRAAEDSAIAIAASRAAVQALDPEIAIANVLLTETAIANTIDAPRFNMA
                     LLTAFAGIALVLAAVGLAAVIGYEVTERTHEIGIRMALGARAENVRRLAMRHGLTPAF
                     LGMVLGVVGALAATKLAASLLYGITPRDPLTFAGVVALLVLVALGAAWLPARRATR"
BASE COUNT          412 a         1052 c         1058 g          552 t
ORIGIN      
        1 ggtggtcgat acgcttcgag gcctcgcgca atgaatcaga tcgaagcgct gcgccgcctg
       61 cgccggttgg gcgcaccggt gttcgagacg cgcgacgcca gtgcgctgct cgaggtgacg
      121 cctgccaacg ccaacatgat cctgcgcagg ctcgcggatc aggatctcat cgtccatctc
      181 gcgcgaggcc gatgggcgat ggggacctcc gtgccgcgct tcgcgctgcc ggagctgatc
      241 tcggcgcctt acccggcgta cgtctccatg caatcggcgc tgtttcaccg cggcctcatc
      301 gagcaggttc cggcggtcat ctacgccgtg acgctcggca agccgcgccg cgtcagcacg
      361 ccgctcggca cgatctcgtt tcaccggctg ccgccggagc tgttccgcgg ctacgagctc
      421 gagccggacg gcagcaagat cgcgacggcg gaaaaagcgc tgttcgacac gctctatttg
      481 gccccagccc gcagccgcct gttcgcgcgc ttgccggagc tcgagattcc gcgtgagttc
      541 cgctggcagg agctgcacga gctcgctgcg cgcgtcaaat tcgaacgacg acgcgtgcat
      601 atcgagcggc gtatcgagca gctcgagcga aggtgaggta cagtcgcgcg agccttccgc
      661 gcagaagccc cattccgagg gatcgatgct gctggatctg aaatccgcgt gccgcgagct
      721 cttgaagaac cgctggttca cctgcgtgac ggtgctgacg ctcgcgcttg ggctaggcgc
      781 gaacacggcg atcttcggcg tcgtcaacaa gctgttgctg aatccgttgc cgtatcccga
      841 ttccgatcgc atcgtctatc tcagagtcgg attgcagcgc gcccaatttg tctttccgcc
      901 accggtcgcc gttgcgagcg cttggcgcga agaagcgcgc tcgttcgagg gattcgaggg
      961 ctcgagctcg aacagcgtgc tcgcgtacga cgagaacggc gcacgcttgc tgcgcgggat
     1021 gcgcttcacg ccgggcttgc cggctttcct cggggtcgcg ccggtcctcg gccgcggttt
     1081 cacgcccgcc gatatcgagc caggcgcgcc ggccgtcgtc atgctgagct acgaaatgtg
     1141 gcagcgcgac tacggcggct cgcgcgacgt ggtcggccgc gcgatcacgc tcgacgactt
     1201 gccgcacgtc gtggtcggcg tgatgccgcc gcgctgggac gcgttcgcgg tcggctttcg
     1261 ccccgaagtg ttgttcccgc aggcttatcc cgtggccggc gcgcagcctg tatccgtcga
     1321 catcatgact cgattgaggg aagacgtgtc gctcgacgcg gcgacgaacg agctcgcggc
     1381 gattctcgcg cgcgtgtcgg ccgaatctcc gcggcccttg ttcggccccg acgctccgac
     1441 cgtgaaaatt cagacaccga cggagcgcac cgcggccaac acgcgcgatg cgctgctggt
     1501 gctgctcgct gccgtcggcc tggtgctgct cgtcgcctgc tccaacgtcg cgaacctgct
     1561 gctcgcgcgc ggcgcgtctc gctcgcgcga gctgtcgttg cgctcggcgc tcggggccag
     1621 cacttggcgg ctcgtgcgcg cgctgttcgc ggagtgcctc gtgctcgcgc tcgcggccgg
     1681 cgtcgtcggc ctcggcgtcg gctggctcac gctacggatc ctcgtcgggc tgcggcccgg
     1741 cagcttgacc gcgatcggcg aggccgagct cgatccgacg gtgctcgcgt tcacgttcgg
     1801 cgtatcggtt ctgaccgcgc tcctgttcgg gctcgcgccg gcgttgcagc tcgcgtcgcg
     1861 caggctcggc gacgcgctgc gcggcggcgc ctcgggcgtc gtgcgcggcg gcatgggccc
     1921 gcgccttcgc aagctgctcg tcgcggcgca gatggcgatc tccgtcgtgc tgctcgtcag
     1981 cgccgggttg ctgattcgca gcttcatcca tttgcagagc gccgagatcg gcttcgacgc
     2041 cgagaacctg ttctccgttc agctgtcgat gccgcgcgcc aaataccaga cgccgacgag
     2101 ccgcgaggtg tttgcggagc agttgctcga gcggcttcgc tcctcgcccg gtgtcacgac
     2161 tgccacgcaa gtcttctccg cgccgccaaa tggcgtgacc ttcgtcggta gctccggctt
     2221 cgagatccgc ggagccagct tgagcgaggc ggacgcgcaa gccgcgcgga tcatgcattt
     2281 cgccggcgcc gactacttca gcgcgcttcg cattgcgctg atcgagggac ggactttcac
     2341 ggcggatgaa atgcgcagca aaacggcggt catcgtgaac cgcgcggccg ccgagcattt
     2401 ctggcccgac ggcgaggctt tgggcggcga gatcaagtgg cagcaagact gggcgacggt
     2461 catcggcgtc gtcgacaacg tcttgttcgg cagcttgacg cgcggccgcg atacgccgca
     2521 gttctactgg cccctcccaa tggggccgag tttcaccggc gggccggcgt cgctgacgtt
     2581 cgtcgtgcgc gcggcagagg attccgcgat cgcgatcgcg gcctctcgag ccgcagtgca
     2641 agcgctggac ccggagatcg cgattgcgaa cgtgctgctg accgagacgg cgatcgcgaa
     2701 cacgatcgac gcgccgcggt tcaacatggc gctgctgact gcgtttgccg gcatcgcgtt
     2761 ggtgcttgcg gccgtcggcc tcgccgccgt gatcggctac gaggtcaccg agcgcacgca
     2821 cgagatcggc atccgcatgg cgctcggcgc gcgcgccgag aacgtgcgcc ggctcgcgat
     2881 gcgccacggg ctcacgccgg cgttcctcgg gatggttctc ggcgtggtcg gggcgctcgc
     2941 cgctactaag ctcgcggcga gcttgctgta cgggatcacg ccgcgcgacc cgctcacgtt
     3001 cgcgggcgtc gtcgcgctgc tcgtgctcgt cgcgctcggc gcggcgtggc tgccggcgag
     3061 gcgcgccacg cgcg
//
LOCUS       DASSNN010000186         3968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1552370, whole genome shotgun sequence.
ACCESSION   DASSNN010000186 DASSNN010000000
VERSION     DASSNN010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552370"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VFL84_03340"
     CDS             <1..1170
                     /locus_tag="VFL84_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET7607686.1"
                     /translation="STKATPPTQPSHYWGNEAIWDSKANAHNPMLDEEGRVWYTAVVR
                     APDNAPAYCRAESGHPSAKLFPLPRSGRQLSMYDPKTEKYTFIDTCYSTHHLQFASDA
                     NDTLWTSGGGPVVGWLNSKKFLETGDAGASQGWTALVLDTNGNGKRDAYVEPGQPADP
                     AKDTRVNSAFYAVMPNPADGSIWGATLGYPGAVVRLDPGSNPPETALAEVYRPPLPGY
                     GIRGGDIDSKGVVWVSMGSGHLGAFDRSKCKGPLNGPTATGDHCPEGWSFYRYPGPGF
                     VGRPEESVEASYYSWVDQHNTTGLGENVPMSTANLFDGVHALVDGEMATLRIPYPLGF
                     YAKGMEGRIDDANAGWKGRGLWVTSGDRTPWLKEGGKGTKPLIVHFQVRPDPLAK"
     gene            1226..1537
                     /locus_tag="VFL84_03345"
     CDS             1226..1537
                     /locus_tag="VFL84_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HET7607687.1"
                     /translation="MKTITMVGLLLVAGAAGAQPPGGGGGLTFDGLDADKNGSLSQDE
                     IAKFFAGRPAGPNGAPNAAEIFGRWDANKDGSVSKAEFDARPRPGGGGGGGPPGGQAP
                     G"
     gene            complement(1542..2327)
                     /locus_tag="VFL84_03350"
     CDS             complement(1542..2327)
                     /locus_tag="VFL84_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7607688.1"
                     /translation="MSFLDFYRSRDGRRVLLYQAVALAAGAVALSLSLGDGHLDLTIA
                     RWFFNDARRLFPLTNDWLLKTVLHDAARTISVVAALALLGLAVTSRLAPKPRGVRAHR
                     DALLFASIATVVAVAVVGALKHFSSHACPWDLAIFGGSAAYHPLLSVHARAQGIDGCF
                     PAAHPLSGYAWLAVGFALYPSARGRAWSAWAAAFALGTLFGAVQIARGAHFLSHVLWS
                     AWVVWAVNLGLLGSLSLLRGARTQAAERTPRRGARDHLASKTS"
     gene            complement(2335..>3968)
                     /locus_tag="VFL84_03355"
     CDS             complement(2335..>3968)
                     /locus_tag="VFL84_03355"
                     /inference="COORDINATES: protein motif:HMM:NF028537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HET7607689.1"
                     /translation="FSRILGAPLLIAAVALFIVVADNGPFWSAALRATHSDEHRLGIL
                     TTLSALVLLPLITVLSLSLGTKLLRVVAALLLLVAASCGYFMTQYGVIIDQSMIRNAV
                     ETTALEATPLLGAAYFLHVALYGVLPAIVVFAVPLARLRWPTEILVRLGTAAAAVLFL
                     AATMYVNYESAVFFGRENDDLRLQINPAYPLWAAATFGMSSDKAAPEVRHPLDVRLAA
                     AAADRKPALVVIVMGETARADRFSFNGYERDTNRYTRERGVVNFPSVVSCGTSTAESL
                     PCIFSGLGHARFSHAAAMASESIVGAMQRLGLDTFWRDNSTGCKDVCDEQHFEQRADW
                     TTPELCDATGCFDELLLEDFDALLADRARDHLIVLHQRGSHGPAYNTDVPQWAKEYFP
                     ECDLPNLRNCDRAAINNSYDNTILYTDYFLSRVIDELEKRSGDFDTAMLYVSDHGESL
                     GENGLYLHGLPHALAPDEQIRVPMLFWASPSFYAERAKVDPQCLRESAQHDTSHDAIF
                     HTLLPIFGLESPLYDERLDLMAACRGELLTAINSR"
BASE COUNT          734 a         1300 c         1372 g          562 t
ORIGIN      
        1 tccacgaagg ccacgccgcc gacgcagccg tcgcactact ggggcaacga agccatctgg
       61 gacagcaaag cgaacgcgca caacccgatg ctcgatgaag aaggccgcgt ctggtacacg
      121 gccgtcgtgc gcgcgcccga caacgcgccg gcgtattgcc gcgcggagtc ggggcacccg
      181 tcggcgaagc tgttcccgtt gccgcgctcg gggcggcaac tgtcgatgta cgacccgaag
      241 acggagaagt acacgttcat cgacacgtgc tactcgacgc accacctgca gtttgcttcc
      301 gacgcaaacg acactttgtg gacgagtggc ggcggccccg tcgtcggctg gctcaactcg
      361 aagaagttcc tcgagaccgg cgacgcgggg gcgtcgcaag gctggacggc gctcgtgctc
      421 gatacgaacg gcaacggcaa gcgcgacgcg tacgtcgagc cgggtcaacc ggccgacccg
      481 gcgaaggaca cgcgcgtcaa cagcgcgttc tatgccgtga tgccgaatcc ggccgacggc
      541 tcgatctggg gcgcgacgct cggctatccc ggcgccgtgg tgcgcctcga tccaggctcg
      601 aacccgcccg agacggcgct cgccgaggtc taccggccgc cgctgcccgg ctacggcatt
      661 cgcggcggcg acatcgactc gaaaggcgtc gtctgggtct cgatgggcag cggccacttg
      721 ggcgcgttcg accgcagcaa gtgcaaagga ccgctgaacg gcccgacggc gaccggcgat
      781 cactgccccg aggggtggag cttctatcgc tatcccggcc ccggcttcgt cggccggccc
      841 gaagaaagcg tcgaggcgag ctattacagc tgggtcgatc agcacaacac gaccggcctc
      901 ggcgagaacg tgccgatgtc gacggcgaac ctgttcgacg gcgtgcacgc gctcgtcgac
      961 ggcgagatgg cgacgctgcg gatcccctat ccgctcggct tctacgcgaa gggcatggaa
     1021 ggccgcatcg acgacgcgaa cgcgggctgg aaaggccgcg gtctctgggt cacgagcggc
     1081 gaccgcacgc cgtggctcaa ggaaggcggc aaaggcacga agccgctcat cgtccacttc
     1141 caggtcaggc cggatccgct cgcgaagtaa cgcgcgagcg tatactcgcc ggcgtttcgc
     1201 aattctcgag ttgggagact ctctgatgaa aacgataacg atggtgggac tcttgctcgt
     1261 cgccggcgca gccggcgcgc agccgcccgg gggcggcggc ggattgacgt tcgacggtct
     1321 cgacgccgac aagaacggct cgctgtcgca ggacgaaatc gcgaagttct tcgcgggacg
     1381 gcctgccgga ccgaacggcg cgccgaacgc agcggaaata ttcggccgct gggatgcgaa
     1441 caaggacggc tcggtctcga aggccgagtt cgacgcgcgc ccgcgaccgg gcggcggtgg
     1501 cggcggcgga ccgcccggcg gccaagcgcc cggctgacgc atcaggaagt tttcgacgcc
     1561 aggtgatcgc gggcccctct tcgaggggtc cgctctgctg cttgcgttct cgcgccgcgg
     1621 agcagcgaca aagaaccgag cagacctaaa ttcacggccc agaccaccca cgccgaccac
     1681 aacacgtgcg agaggaagtg tgcgccgcgc gcgatctgca cggcaccgaa tagcgttccc
     1741 aaagcgaagg ccgctgccca cgccgaccag gcccgcccgc gcgcgctggg atagagcgcg
     1801 aatccaacgg cgagccacgc gtatcccgag agcggatgcg cggcggggaa gcagccgtcg
     1861 atgccctgcg ccctcgcgtg cacgctgagc agcgggtgat acgcggcgga gccgccgaat
     1921 atcgcgagat cccacgggca cgcgtgcgag ctgaaatgct tcaacgcgcc aacgaccgca
     1981 acggcaacga ctgtcgcgat cgacgcgaac agcagcgcat cgcggtgcgc gcgaacgcct
     2041 ctcggctttg gcgcgagccg gctagtgacg gcgaggccga gcaacgcgag cgcggccacg
     2101 acgctgatcg tgcgagcggc gtcgtgcagc acggtcttca gcagccagtc attggtcagc
     2161 gggaacaggc gccgcgcgtc gttgaagaac cagcgagcaa tcgtgagatc cagatgcccg
     2221 tcgccgagac tcaagctcag cgcgacggcg ccggcggcga gcgcgacggc ctgatagagg
     2281 aggacgcgtc ggccatcgcg gctgcgataa aagtcgagga aggacatgct cggctcagcg
     2341 gctgttgatc gccgtgagca gctctccgcg gcaggccgcc atgaggtcga gccgctcgtc
     2401 gtacagcggc gactcgaggc cgaagatcgg caacagcgta tgaaagatcg cgtcgtggct
     2461 cgtgtcgtgc tgcgcgctct cgcgcaagca ttgcggatcg accttcgcgc gctcggcgta
     2521 gaagcttggg ctagcccaga acagcatcgg cacccggatc tgctcgtccg gggccagcgc
     2581 atgaggcaaa ccgtgcaaat acagcccgtt ctcgccgagc gactcgccgt gatcggacac
     2641 gtacagcatc gccgtatcga aatcgccgct gcgtttctcg agctcgtcga tgacgcggct
     2701 caagaaatag tcggtgtaga ggatcgtgtt gtcgtaggag ttgttgatcg ccgcgcggtc
     2761 gcagttgcgc aggttcggca gatcgcattc ggggaaatac tccttggccc actgcggcac
     2821 gtccgtgttg taagccgggc cgtggctgcc gcgctggtgc agcacgatca gatgatcgcg
     2881 cgcgcgatcg gcgagcagag cgtcgaagtc ttccagcagc agctcgtcga agcagcccgt
     2941 ggcatcgcag agctcagggg tcgtccagtc ggcgcgctgc tcgaagtgct gctcgtcgca
     3001 gacgtccttg cagcccgtgc tgttgtcgcg ccagaacgtg tctagcccca agcgctgcat
     3061 cgcgccgacg atgctctcgc tcgccatcgc ggcggcgtgc gagaagcgcg cgtggccgag
     3121 gcccgagaag atgcacggca gcgactcggc tgtcgaggta ccgcacgaga cgacgctcgg
     3181 aaaattcacg acgccgcgct cgcgcgtgta gcgattcgtg tcgcgctcgt agccgttgaa
     3241 cgagaagcga tcggcgcgcg cggtctcgcc cattacgatc acgaccagcg ccggcttgcg
     3301 gtcggctgcg gccgccgcga gccggacgtc gagtggatgc cgcacttccg gcgccgcttt
     3361 atccgagctc atgccgaacg tcgcggcggc ccacaacgga tacgccgggt tgatctgcaa
     3421 gcgcaggtcg tcgttctcgc ggccgaagaa cacggcggat tcatagttca cgtacatcgt
     3481 cgccgccaag aacagcacgg cggcggccgc agtgccgagg cgcaccaaga tctccgtcgg
     3541 ccagcgcagc cgcgcgagcg gcacggcgaa cacgacgatc gcgggcaaga cgccgtacag
     3601 cgcgacgtgc aagaagtacg ctgcgccgag cagcggcgtc gcttcgagcg ccgtcgtctc
     3661 gacggcgttg cggatcatcg actggtcgat gatcacgccg tactgcgtca tgaagtaccc
     3721 gcagctcgcc gcgacgagca gcagcagcgc ggcgacgacg cgcagcagct tcgtgccgag
     3781 cgagagcgac agcacggtga tcagcggcaa caagaccaat gcggagagcg tggtcaggat
     3841 cccgagccgg tgctcgtcgg agtgggtcgc gcgcagcgcg gcgctccaga agggaccgtt
     3901 gtctgcaacg acgatgaata gagcgacggc cgcgatcagc agcggggcgc cgagaatgcg
     3961 gctgaagc
//
LOCUS       DASSNN010000187         3276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1047439, whole genome shotgun sequence.
ACCESSION   DASSNN010000187 DASSNN010000000
VERSION     DASSNN010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047439"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..785)
                     /gene="crp"
                     /locus_tag="VFL84_03360"
     CDS             complement(96..785)
                     /gene="crp"
                     /locus_tag="VFL84_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HET7607690.1"
                     /translation="MAEEALRPLSDVPAINRFLSYCRVRTIPGKTVMIHAGDLPDVLY
                     YIVDGSVEVMIEDEDGNEMVLAYLNKGQFFGEMGLFYEQPQRSAWVRTRSESEIAEMT
                     YPRFRQIASESPGLVFELATQLATRLDRTNRKLGDLAFVDVTGRVAHAIMDLCREPDA
                     MTHPDGMQIKVSRQELSRLVGCSREMAGRVLKVLEDQGLVRASGKTIVVFNARRELPR
                     ARAAEASLRRA"
     gene            complement(872..1510)
                     /gene="coq7"
                     /locus_tag="VFL84_03365"
     CDS             complement(872..1510)
                     /gene="coq7"
                     /locus_tag="VFL84_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HET7607691.1"
                     /translation="MPTALSWPDRLITAVEQGLRAVAAPPLATRPSPAAALPEPALSA
                     GERRTSAALMRVNHAGEIAAQALYSGQSLFARSPATRALLANAAREERDHLAWCAARI
                     EELGGRTSLLTPLWYAGSFGVGVLASLRDDVDSLGFVRETERQVEAHLRDHLGRLPEH
                     DEKSAAILSRMAEDEVHHGTTALLAGGTELPAAIRAGMKIGGEILRRIALIV"
     gene            1627..2055
                     /gene="rplM"
                     /locus_tag="VFL84_03370"
     CDS             1627..2055
                     /gene="rplM"
                     /locus_tag="VFL84_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET7607692.1"
                     /translation="MKTISAKPDSVRRDWYLVNAEGKTLGRLATALAHRLRGKHKPIY
                     TPHVDTGDFIVVINAEKIRVTGSKLKDKIYYRHTGTIGNMKREPLEKVLATHPERAIE
                     IAVKGMLPKSPLGRQMFKKLKVVRGDKHPHAAQQPKPLEL"
     gene            2060..2452
                     /gene="rpsI"
                     /locus_tag="VFL84_03375"
     CDS             2060..2452
                     /gene="rpsI"
                     /locus_tag="VFL84_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET7607693.1"
                     /translation="MAIEINYGTGRRKTSAARVYLKPGNGDIVVNGRPLDVFFGRETA
                     RMIVRQPLVVAEAEGRFDVQVTVRGGGTTGQAGAIRHGITRALMDYDESLRKTLRKAG
                     FVTRDAREVERKKVGLRKARRRPQYSKR"
     gene            2537..2610
                     /locus_tag="VFL84_03380"
     tRNA            2537..2610
                     /locus_tag="VFL84_03380"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2569..2571,aa:Gln,seq:ctg)
     gene            complement(2621..>3276)
                     /gene="rpiA"
                     /locus_tag="VFL84_03385"
     CDS             complement(2621..>3276)
                     /gene="rpiA"
                     /locus_tag="VFL84_03385"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET7607694.1"
                     /translation="VSQDDNKRRAAAAALPYIKDGMSLGIGTGSTIDILIELLQPLKS
                     RLGPVVSSSQRSTRRLEDFGIRTVELRSTGDLDLYIDGADEATKHRHLIKGGGGALTQ
                     EKVVAAASRRFVCIIDETKLVATLGRFPLPIEVIPMAESYVARQMIKARGQPILREGF
                     VTDNGNHILDVHNLKIANPVELETRFNQIPGVVTVGIFANRPADVLLIGSASGVKTL"
BASE COUNT          586 a         1051 c         1079 g          560 t
ORIGIN      
        1 ttgcggagca gaagacgcac gaggtgcgga cggcttacga gcggataaag acgcagcgcg
       61 gcttcaagta gccgcgccac gcctgggaag acggactagg cgcgccgcag cgacgcctcg
      121 gctgcgcggg cacgcggcaa ctcgcggcgc gcgttgaata cgacgatcgt cttgccgctc
      181 gcgcggacga ggccttgatc ctcgagaacc ttgagcacgc ggccggccat ctctcgcgag
      241 cagccgacga gacgcgacag ctcttggcgc gataccttga tctgcatgcc gtcgggatgc
      301 gtcatcgcgt cgggctctcg gcacagatcc atgatcgcgt gcgccacgcg gccggtgacg
      361 tcgacgaagg cgaggtcgcc gagcttgcgg ttcgtgcgat cgaggcgcgt cgcaagttgc
      421 gtcgcgagct cgaacacgag gccggggctt tcggaggcga tctgccgaaa ccgcggatac
      481 gtcatctcgg cgatttcgct ctccgaacga gtgcgcaccc aagcgctgcg ttgcggttgc
      541 tcgtagaaca accccatctc gccgaagaac tgacctttgt tgaggtaggc gaggaccatt
      601 tcgttgccgt cctcgtcctc gatcatcact tcgacggagc cgtcgacgat gtagtagaga
      661 acgtcgggta agtccccggc gtgaatcatg acggtcttgc ccggaatcgt gcgcaccctg
      721 cagtagctca ggaagcggtt gatcgcggga acgtcgctca agggcctgag agcttcttca
      781 gccatacgtc gggactccgg tttgactaaa cgcagtattt aatcaattcc ctcgtgtcgc
      841 gcaagcgaga catgtaaagc tgtgacaccg atcacacgat aagcgcaatt ctgcgcaaaa
      901 tctcgcctcc gatcttcatt ccggcgcgaa ttgcggccgg caactcggtg ccgccggcaa
      961 gcagcgccgt cgtgccgtga tgcacttcgt cttctgccat gcgcgagagg atcgcggcgc
     1021 tcttctcgtc gtgctcgggc aggcgtccca agtggtcgcg caagtgagct tcgacctgcc
     1081 gctcggtctc gcgcacgaag cccaagctgt cgacgtcgtc gcgaaggctc gcgagcactc
     1141 cgactccgaa gctgcccgca taccacagcg gcgtgagcag gctcgttcgc ccgccgagct
     1201 cctcgatcct cgccgcgcac cacgcgaggt gatcgcgctc ctcgcgggcc gcgttcgcca
     1261 acagcgcgcg cgtcgcgggc gagcgcgcga acaaggactg gccgctgtag agcgcttgcg
     1321 ccgcgatctc gcccgcgtga ttgacgcgca tcagcgcggc gctcgtgcgg cgctcgccgg
     1381 ccgagagggc gggctccggc agcgcggcgg ccggggaagg gcgcgtcgcg aggggcggcg
     1441 cggcaacggc gcgcaggccc tgctcgacgg cggtaatcaa gcggtccggc caggagaggg
     1501 cggtcggcat gaggccatta tattgagggc tcgaagcgcg gcggtggttg cgccgccccg
     1561 ggtcggccag taagattccg cctctttggc cggccgggcg ccggctccag caagagctta
     1621 ccggacatga agaccatttc tgccaagccg gactccgttc ggcgcgattg gtacctcgtc
     1681 aacgcggagg gcaaaacgct cggccgcctg gcgaccgcgc tcgcgcaccg gctgcgcggc
     1741 aagcacaagc cgatctacac gccgcacgtc gatacgggcg acttcatcgt cgtcatcaac
     1801 gccgagaaga tccgcgtgac gggctccaag ctcaaagaca agatctatta ccgccatacg
     1861 ggcacgatcg gcaacatgaa gcgcgagccg ctcgagaagg ttctcgcgac gcatcccgag
     1921 cgagcgatcg agatcgccgt gaaaggcatg ctgccgaaga gcccgctcgg acggcagatg
     1981 ttcaagaagc tcaaggtggt ccgcggcgac aagcatccgc acgccgcgca gcagccgaaa
     2041 ccgttggagc tctaacgata tggccatcga aatcaactac ggcaccggac ggcgcaagac
     2101 ctcggcggcc cgcgtttacc tgaagcccgg caacggcgac atcgtcgtga acggccggcc
     2161 gctcgacgtg ttcttcggcc gtgagacggc gcgcatgatc gtgcgccaac cgctcgtcgt
     2221 ggccgaggcc gaagggcgtt tcgacgtgca agtcaccgtt cgcggcggcg gcacgaccgg
     2281 ccaggccggc gcgattcggc acggcatcac gcgcgcgctc atggattacg acgagagttt
     2341 gcgcaagacg ctgcgcaagg ccggtttcgt cactcgcgac gcgcgcgagg tcgagcgcaa
     2401 gaaggtgggc ttacgcaaag ctcgccgacg cccgcagtac tcgaagcgct gatcaaaaag
     2461 gggacggatt tattttcctc cgaaaataga tctgtccccg tttagcgttg tgctagcgtt
     2521 gtagatgcct cacgactggg ggatcgtcca gcggtaggac atcggactct gactccgaga
     2581 acctaggttc gaatcctagt cccccagcca cctcgcttct tcaaagtgtc ttgacgccgc
     2641 tcgcgctgcc gatcagcagc acgtccgcgg ggcggttcgc gaagatcccg accgtcacga
     2701 cgcccggtat ttgattgaac cgcgtctcga gctcgacggg gttcgcgatc ttcaaattgt
     2761 gcacgtcgag gatgtggttg ccgttgtcgg tgacgaagcc ttcgcgcagg atcggctgcc
     2821 cgcgcgcttt gatcatttgc cgcgcgacat aggactccgc catcggaatc acttcgatgg
     2881 gcagcgggaa ccggccgagc gtcgccacca gcttggtctc gtcgatgatg cagacgaagc
     2941 ggcgcgaggc ggcggccacg actttctcct gcgtcagcgc gccgccgccg cccttgatca
     3001 ggtgccgatg cttggtcgct tcgtcggcgc cgtcgatgta gagatcgagg tcgcccgtcg
     3061 agcgcagctc gaccgtccgg atgccgaaat cctcgaggcg ccgcgtcgat cgctgcgagc
     3121 tcgagacgac ggggccaagc ctcgacttga gcggttgcaa gagctcgatc aggatgtcga
     3181 tggtgctgcc ggtgccgatg ccgaggctca tgccgtcctt gatgtagggc aacgctgcgg
     3241 ccgcggcacg gcgtttgttg tcgtcttggc tcacga
//
LOCUS       DASSNN010000188         3235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_650503, whole genome shotgun sequence.
ACCESSION   DASSNN010000188 DASSNN010000000
VERSION     DASSNN010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650503"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..707
                     /locus_tag="VFL84_03390"
     CDS             54..707
                     /locus_tag="VFL84_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607695.1"
                     /translation="MANNSVDRRAARALVPVLVALGSLVASPVHAEVKGSWSDGVSRE
                     QSFARVLVVGISPDLNQRCAFERSMAAQLKSASTQAFVSCDAIPRDTPVSRESIVAAV
                     AAKNADAVLVTSFVSQSSGVEEGGTRDTRGVAVYKATDAYLDGYGGVVAADFHTAAPS
                     TGIKGEAHLGSKLFETSGATVVYTLDTKVRGIESRGQALAEIAPPVAKKLRKEGLIR"
     gene            776..1225
                     /locus_tag="VFL84_03395"
     CDS             776..1225
                     /locus_tag="VFL84_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607696.1"
                     /translation="MDDAHLERLIRRAGYDIRSEDGPGDGSHGVYALRDGKRHTRSQP
                     NLLALAEHLGCVERPVQFVEDRDALDRVIAEHAQERNHFVVRRAACRVALYRRYKYVQ
                     LVSVTGSAPAMYELGVDGLRRTSNGNLPPEVVAAFAQPVRSVTSVTV"
     gene            complement(1222..1494)
                     /locus_tag="VFL84_03400"
     CDS             complement(1222..1494)
                     /locus_tag="VFL84_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607697.1"
                     /translation="MTNFKSAAKRKRELAKLDKRHAKDQKRALRRAERSAADVGAPAP
                     AVAPVAQPMQREHRLAPRVAPVSKPLTLAEAVERWKNTKVVKPKTR"
     gene            complement(1570..1887)
                     /locus_tag="VFL84_03405"
     CDS             complement(1570..1887)
                     /locus_tag="VFL84_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET7607698.1"
                     /translation="MGRKLYVGNLPYSATEQSLRDAFAASGTVDSVSLITDRDTGQSK
                     GFAFIEMSSDSEAQTATQNMNGTMLDGRQIKVNEAKPRENRGGGNRSGGFGGGGGGYG
                     RRY"
     gene            2113..2373
                     /locus_tag="VFL84_03410"
     CDS             2113..2373
                     /locus_tag="VFL84_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET7607699.1"
                     /translation="MGTRLYVSNLPLSATEERLGSQFGKFGVVLAVALDAVVRASRRG
                     AFVEMETSADAHRAIAALNLSDFDGCLVSVYLARASAPVKPA"
     gene            2370..2672
                     /locus_tag="VFL84_03415"
     CDS             2370..2672
                     /locus_tag="VFL84_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607700.1"
                     /translation="MMLDETRPEETDDDAERYRLVSIDAVGAPDGCSGTDWHVYRIAQ
                     GDNEITGYRNGALARVTTDVETIVTALNERRRWSKSKTPSKYQRRAAAAARRGAAR"
     gene            2694..2942
                     /locus_tag="VFL84_03420"
     CDS             2694..2942
                     /locus_tag="VFL84_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET7607701.1"
                     /translation="MSTSIYVSNLAASTTKETLILKFSEFGTVLSVKLGRDPESGQAM
                     PYGFVEMKTSDEARSAARSLNATRLDGRLLSVHRAARS"
     gene            2999..>3235
                     /locus_tag="VFL84_03425"
     CDS             2999..>3235
                     /locus_tag="VFL84_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6164 family protein"
                     /protein_id="HET7607702.1"
                     /translation="MSKLLLNLRDVPDDEADDVRRFVDANAIGYYETRPSFWGVSAGG
                     IWIRDDRDLAVAKRLMAEYQRARQVRAREQWAEAE"
BASE COUNT          561 a         1040 c         1083 g          551 t
ORIGIN      
        1 cacgagcctg tgaccgcggg agactcgggt gcgctgcgta agaggaaagt tccgtggcca
       61 acaactccgt cgatcgacgc gccgctcgcg ccctcgtgcc ggtgctcgtc gccttgggct
      121 cgctcgtcgc ttcgccggtc cacgcagagg tcaagggctc gtggtccgac ggcgtctcgc
      181 gagagcagtc gttcgcgcgc gtgctcgtcg tcggcatcag ccccgatctc aaccagcggt
      241 gcgcgttcga gcgctcgatg gcggcgcagc tgaagagcgc aagcactcag gcgttcgtga
      301 gctgcgatgc aatccctcga gacacgccgg tctcgcgcga gagcatcgtg gccgccgtcg
      361 ccgcgaaaaa tgcagacgcg gtgctcgtca cgagcttcgt ttcccaatca tcgggggtcg
      421 aggaaggcgg aactcgcgat acgcgaggcg tcgccgtgta caaggccacg gacgcctatc
      481 tcgacggcta cggcggcgtc gtcgccgccg atttccacac ggccgctccg tcgacgggga
      541 tcaaaggcga ggctcacctc gggtcgaagc tgttcgagac gagcggcgcg acggtcgtgt
      601 acacgctcga cacgaaggtc cgcggcatcg agtcgcgcgg ccaagcgctc gcggagatcg
      661 ctccacccgt cgccaagaag ctgcgtaagg aaggattgat tcgttgatgt cgcgggtcga
      721 tcggcggcca ccaaagaacc acgccgtcac ggtctggctc gtgctagggt gagccatgga
      781 cgacgcccac ctcgaacgac tgatccgtcg ggcggggtat gacattcgtt cggaagacgg
      841 gccgggcgat ggctcccatg gagtctacgc gctgcgcgac ggcaagcggc acacgcgatc
      901 gcaaccgaac ctgctcgcac tcgccgagca tttgggttgc gtggaaaggc cggtgcaatt
      961 cgtcgaggac cgcgatgcgc tcgaccgagt catcgcggag cacgcgcaag agcgcaatca
     1021 cttcgtcgtg cgccgcgccg cgtgccgcgt cgcgctgtac cgtcgataca agtacgtgca
     1081 gctcgttagc gttacgggca gcgcgccggc gatgtacgag ttgggcgtgg acgggcttcg
     1141 gcgcacgagc aacggcaacc tgccacccga agtcgttgcg gcgttcgcgc aacccgtgag
     1201 gagcgtgacg agcgtaacgg tctagcgtgt cttcggcttc acgactttcg tgttcttcca
     1261 tcgctcgact gcttcggcga gcgtcagcgg cttgctcacg ggagccactc ttggcgcaag
     1321 ccgatgctca cgctgcatcg gctgtgcgac gggcgcgacg gcgggggccg gcgcgccgac
     1381 gtccgcggcc gatctctcgg ccctgcgcag agcgcgtttc tggtccttcg cgtgccgctt
     1441 gtcgagcttg gcgagctcgc gcttgcgctt cgccgccgac ttgaaattgg tcatggcgtt
     1501 cctcccaaag ggctaggacc tgccgcgccc aacgaggcgc ggcagggagc tggagccgct
     1561 tgcgaaaagt tagtagcgcc ggccgtagcc gccgccaccg cccccgaaac cgccgctgcg
     1621 attgccaccg ccgcggttct cgcgcggctt cgcttcgttg accttgatct ggcggccgtc
     1681 gagcatcgtg ccgttcatgt tttgcgttgc ggtttgcgcc tcgctgtcgg acgacatctc
     1741 gatgaacgcg aagcccttgc tctggcccgt gtcccgatcg gtgatcaagc tcacgctgtc
     1801 caccgtgccg ctcgccgcga aagcatcgcg caacgactgc tcggtcgcgg aatacggcag
     1861 attgccgacg taaagtttcc tgcccatggt tacctcctca ggtatcgttc gcgtcaggag
     1921 gatgaaggcg ggatgcccgc aactcgcgag aagcgatagg gtgtgcggca ctatacgcct
     1981 tgcacgccgc gagcgttgtc ttattttcgc gtcgccggga cgagctcttg agaaaccgcc
     2041 gctcgcgcta ggcaagtcgt gtaaacttca cgtagccaga ctccgctggc ggttcccacc
     2101 aaggacacgc gtatgggcac acggctctac gtaagcaact tgccgctgtc tgcgacggag
     2161 gaaaggctcg gcagccaatt cggcaagttc ggcgtcgttc tcgcggtcgc gctcgacgcc
     2221 gtggtgcgcg cgagccggcg aggagcgttc gtcgagatgg agacgagcgc cgatgcgcac
     2281 agagcgatcg cggcgctcaa tctttccgat ttcgacggtt gtctcgtatc cgtctatctc
     2341 gcacgcgcga gcgcgccggt gaagcctgca tgatgctcga cgaaactcgg ccggaagaga
     2401 ccgacgacga cgccgaacgt tatcggctgg tatcgatcga tgcggttggc gcgcccgacg
     2461 gctgcagcgg gaccgactgg cacgtctatc gaatcgcgca aggggacaac gagatcaccg
     2521 gctatcgaaa cggcgcgctc gcccgcgtga ccacggacgt cgagacgatc gtgaccgcgc
     2581 tcaacgaaag acgacgctgg tcgaagagca agacgccgtc caagtatcag cgtcgggccg
     2641 cagccgccgc gcgccgcgga gcggccaggt gaacggctag cgagagaacc gtcgtgagca
     2701 catcgatcta cgtgagcaat ctggccgcat cgacgaccaa agagacgctc attttgaagt
     2761 tcagcgagtt cggaaccgtg ctatcggtga agctcggccg cgatcccgag agcggccagg
     2821 cgatgcccta cggcttcgtc gagatgaaga cctcggacga ggcgcgatcc gcggcgcgtt
     2881 cgctgaacgc gactcgcctc gacggccggt tgctctccgt gcatcgtgcc gcgaggagct
     2941 aagcgcaagc cttgagcagc gcaagagcgg cgagcgtctc gtccgctatg atcgcggcat
     3001 gtcgaagctg ctgctgaact tgcgcgacgt gcccgacgac gaggccgacg acgtccgccg
     3061 cttcgtcgac gcgaatgcga tcggctacta cgaaacgcgg ccgagctttt ggggcgtctc
     3121 ggccggcggc atctggatcc gcgacgaccg agatctcgcc gtcgcgaagc gactgatggc
     3181 cgagtaccag cgtgcgcgcc aagtgcgcgc acgcgagcaa tgggccgagg cagaa
//
LOCUS       DASSNN010000189         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1048236, whole genome shotgun sequence.
ACCESSION   DASSNN010000189 DASSNN010000000
VERSION     DASSNN010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048236"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..214)
                     /locus_tag="VFL84_03430"
     CDS             complement(71..214)
                     /locus_tag="VFL84_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607703.1"
                     /translation="MAYFLFAGDMFVMAFMAGVVVWVSIRASDASIRASARIPLEDED
                     VHG"
     gene            complement(217..849)
                     /gene="ccoO"
                     /locus_tag="VFL84_03435"
     CDS             complement(217..849)
                     /gene="ccoO"
                     /locus_tag="VFL84_03435"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HET7607704.1"
                     /translation="MLRLHQALERNTGLLIFGILFVSSIGGLVQVLPTIVDDSLSTPA
                     ANVVPHTPLELVGRDVYIREGCATCHSQQVRPLLAEVKRYGQYAGAGEFAYERPFLWG
                     SKRTGPDLLREGGKYSDQWHRIHLTNPRAVVPASIMPAYPWLAERRADDGTVTARLNA
                     LRKLGHPYSDEDVEAAPAALADKTELDGLIAYLQSLGLHAAAATDAHGGH"
     gene            complement(852..>2014)
                     /locus_tag="VFL84_03440"
     CDS             complement(852..>2014)
                     /locus_tag="VFL84_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7607705.1"
                     /translation="RLAWFVFFAWQLTIVLGGWSLLAGWNGGKEYAELEWPFDIAIAI
                     VWLSFAVVFFGTIARRKIAPIYISNWFYGALIIVIAMLHVVNNLALPATLGKAYSLYP
                     GAQDAIVQWWYGHNAVGFLLTGGFLGMLYYFLPKQAEQPIWSYRLSIVAFWAFVYSYI
                     WAGPHHLHYNAIPEWVQSLAVVMSLILLAPSWATMINGVMTVANAWQKLRTDPALKFI
                     VLALAFYGLATFEGPMMAIRSVNVMSHFTEWTIGHVHSGALGWNALITFGTFYYLVPR
                     LAGRPLASVKLANAHFWLALAGTMLYILSMWAAGISQSLLWLTLDEHGEVKYSFVEVM
                     RAIAPYYLLRLLGGLLFLAGAVLMAWNLLKTFEGARTVAARVPAIDAAPQVA"
BASE COUNT          384 a          651 c          680 g          299 t
ORIGIN      
        1 ccaggcgagc cctacgcctg acacgatcgt gatgaccacg atccatccgc tccagaaatc
       61 ggacggcagg tcagccatgc acatcctcgt cttcgagcgg tatgcgcgcc gaagcgcgga
      121 tgctcgcgtc gctcgcacgg atcgagaccc agacgacgac gccggccatg aacgccatca
      181 cgaacatgtc gccggcgaag aggaaatacg ccatcgctag tgtcctccgt gcgcgtcggt
      241 cgccgccgcg gcgtgtaacc caagcgactg caggtaggcg atgagcccgt cgagctcggt
      301 cttgtcggcg agcgccgcgg gcgccgcttc gacgtcctcg tcggaatacg ggtgcccgag
      361 cttgcgcagc gcgttgagcc gcgcagtgac ggtgccgtcg tcggcccggc gctccgcgag
      421 ccacgggtac gccggcatga tcgacgccgg cacgacggcg cgcggattcg tcaggtgaat
      481 gcggtgccac tggtcgctgt acttgccgcc ctcgcgcagg aggtcgggtc ccgtgcgctt
      541 cgagccccag aggaacgggc gctcgtacgc gaactcgccg gcgcccgcgt actggccgta
      601 acgcttgacc tccgcgagca gggggcgcac ctgctgcgag tggcacgtcg cgcaaccttc
      661 acggatgtag acgtcgcggc cgacgagctc gagcggcgtg tgcggcacga cgttcgcggc
      721 cggcgtggat agcgagtcgt cgacgatcgt cggcaacact tgcacgagac cgccgatcga
      781 ggagacgaac aggatcccga atatcaggag accggtgttt cgctcgagcg cttggtgcag
      841 gcgcagcatg ctcaggccac ctgcggtgca gcgtcgatcg ccggcacgcg tgccgcgacg
      901 gtgcgcgcgc cctcgaacgt cttcaaaagg ttccacgcca tcagcacggc gccggcgagg
      961 aacagcagtc cgcccagcaa gcgcagcaag tagtacggcg cgatcgcgcg catgacctcg
     1021 acgaagctgt acttgacctc gccgtgctcg tctagcgtga gccagagcag cgactgcgag
     1081 atgccggcgg cccacatcga gaggatgtag agcatcgtgc ccgcgagagc gagccagaag
     1141 tgcgcgttcg cgagcttcac gctcgcgagc ggccggccgg cgagcctcgg cacgaggtaa
     1201 tagaacgtgc cgaacgtgat cagcgcgttc cagccgagcg cgccggaatg aacgtggccg
     1261 atcgtccact ccgtgaagtg gctcatgacg ttgacgctgc ggatcgccat catcggcccc
     1321 tcgaacgtcg cgaggccgta gaacgcgagc gcgagcacga tgaacttcag cgcgggatcg
     1381 gtacgcagct tctgccacgc gttcgcgacg gtcatgacgc cgttgatcat cgtggcccac
     1441 gacggcgcga gcaggatcag gctcatgacg acggcgagcg actgcaccca ttcgggaatc
     1501 gcgttgtagt gcaggtggtg cgggccggcc cagatgtagc tgtagacgaa cgcccagaac
     1561 gcgacgatcg agaggcgata gctccagatc ggctgctcgg cttgcttcgg caagaagtaa
     1621 tagagcatgc cgaggaaacc gcccgtgagc aggaagccga cggcgttgtg gccgtaccac
     1681 cattgcacga tcgcgtcctg cgctcccgga tacagcgagt acgccttgcc gagcgtcgcg
     1741 ggcagggcca agttgttcac gacgtgcagc atcgcgatga cgatgatcag cgcgccgtag
     1801 aaccagttcg agatgtagat gggtgcgatc ttgcggcgcg cgatcgtgcc gaagaacacg
     1861 actgcgaagg acagccagac gatggcgatc gcgatgtcga acggccactc gagctccgcg
     1921 tactccttgc cgccgttcca cccagcgagc agcgaccagc cgccgagcac aatcgtgagc
     1981 tgccaggcaa agaatacgaa ccatgcgagg cgcg
//
LOCUS       DASSNN010000190         2440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_273617, whole genome shotgun sequence.
ACCESSION   DASSNN010000190 DASSNN010000000
VERSION     DASSNN010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273617"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /gene="folP"
                     /locus_tag="VFL84_03445"
     CDS             <1..839
                     /gene="folP"
                     /locus_tag="VFL84_03445"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET7607706.1"
                     /translation="EHARAGGHHLPLWMGIVNLTPDSFSDGGELVDDAAVEARVAKLV
                     GDGAEIIDLGAESTRPGAAPLTVDEEWARLEPALGRLVDRYSHSLLRPRLSVDTYHDE
                     TARRALALGADIVNDVSGLTTPAMIGLAATSGAEFVAMHNLGVPADKARTLPANEDPT
                     AAVERWLEERLREWERAGIDASRIVFDPGVGFGKNALQSLRVLRNVERLHRFGLRLLV
                     GHSRKSFMHHVATTDRLDRDLFTVGASLRLAAAGVDILRVHNVAAHAGAYRGWAHVEP
                     KR"
     gene            complement(844..1254)
                     /locus_tag="VFL84_03450"
     CDS             complement(844..1254)
                     /locus_tag="VFL84_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607707.1"
                     /translation="MDDPTQHEPSDRVWLGRLLLLYLTASLLHFAHNAEYVSAYPNLP
                     AWLSRADVYLAWLGLAAIGAAGWLLYRSGWRLAGLLAIGAYAVFGFDGLLHYTRAPFA
                     AHTPAMNFTISFEVAAAALLLVGVLKLVVTRRAR"
     gene            complement(1258..1839)
                     /locus_tag="VFL84_03455"
     CDS             complement(1258..1839)
                     /locus_tag="VFL84_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HET7607708.1"
                     /translation="MLALCACTPNAGYEPHDGDIVFQTSRSSQSLAIQLATKSPYSHM
                     GIVYVRDGQPFVFEAVQPVKLTPLTEWIERGERGHFVAKRLSDADARLTLDTLRKMRA
                     VGEQLAGKDYDPYFEWSDDRIYCSELVWKVFECGAGIELGELQTIAAFDLSHPAVQAK
                     VRERYGDHVPLDEIVVSPAAIFDAAGLEIVFER"
     gene            complement(1884..2135)
                     /locus_tag="VFL84_03460"
     CDS             complement(1884..2135)
                     /locus_tag="VFL84_03460"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HET7607709.1"
                     /translation="MLPRRKLTCSFCGKSAAQVAKLVAGRRGYICDVCAAEAHRIMSE
                     WDSAAPLPPAPPSIGARIRRLLSRLSNDGSSTRALHRPA"
     gene            complement(2172..>2440)
                     /locus_tag="VFL84_03465"
     CDS             complement(2172..>2440)
                     /locus_tag="VFL84_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607710.1"
                     /translation="LSALDWSLLFAASALLLTSMVMLFRLRRSSLYWFGAYLTLGALA
                     SVWYVLTPEREAYFDVRVTSSSALPVAVAVLLYMLRLKRRTVLA"
BASE COUNT          451 a          827 c          810 g          352 t
ORIGIN      
        1 tcgagcatgc gcgcgccggc ggacaccact tgccgctctg gatgggcatc gtgaatctga
       61 cgccggactc gttctcggac ggcggcgagc tcgtcgacga cgccgcggtc gaagcacgcg
      121 tcgcgaagct cgtcggcgac ggcgcggaga tcatcgacct cggcgccgag tcgacgcggc
      181 ccggcgccgc gccgttgacc gtcgacgaag aatgggcgcg gctcgagccc gcgctcggcc
      241 gcctcgtcga ccgctatagc cactcgctgc tgcggccgcg cttgagcgtc gacacctacc
      301 acgacgagac ggcgcggcgt gcgctcgcgc tcggcgccga catcgtcaac gacgtcagcg
      361 ggctcacgac gccggcgatg atcgggctcg ccgccacgag cggcgccgag ttcgtcgcga
      421 tgcacaacct cggcgtgccc gccgacaagg cgcgcacgct gccggcgaac gaggacccga
      481 ccgcggccgt cgagcgctgg ctcgaggagc ggctgcgcga gtgggagcgc gccggcatcg
      541 acgcgtcacg catcgtgttc gatccggggg tcggcttcgg caagaacgcg ctgcaatcgt
      601 tacgcgtgct gcgcaacgtc gaacgattgc atcgcttcgg tttgcgttta ctcgtcggcc
      661 actcacgcaa gtcgttcatg caccacgtcg cgacgaccga ccgcctcgac cgcgatctgt
      721 tcacggtcgg tgcatcgctg cggctcgcgg ccgcgggcgt cgacatcttg cgcgtgcaca
      781 acgtggccgc gcacgccggc gcctatcgcg gctgggcaca cgtcgaaccg aagaggtgag
      841 gcgctacctc gcgcgtcggg tcacgacgag cttcaacacg ccgaccagca acagggccgc
      901 tgccgcgacc tcgaacgaga tcgtgaagtt catggccggc gtatgggccg caaatggcgc
      961 tcgcgtgtag tggagcagac cgtcgaagcc gaagacggcg tacgcgccga tcgcgagcaa
     1021 ccccgcgagg cgccagccgc tgcgatatag cagccagcct gccgctccga tggcggccaa
     1081 accgagccac gcgagataga catcggcgcg gctcagccac gccggcaagt tgggataagc
     1141 cgaaacgtat tcggcgttat gcgcgaaatg gagcaggctc gcggtcagat agaggagcag
     1201 caaccggcct agccacacgc gatcggacgg ctcatgttgc gtgggatcgt ccattgctca
     1261 gcgctcgaac acgatttcca agcccgccgc gtcgaagatg gcggctggcg atacgacgat
     1321 ctcgtcgagc ggaacgtgat cgccgtatcg ctccctgact ttcgcctgca cggccgggtg
     1381 cgagagatcg aacgcggcga tcgtctgcag ctcgccgagc tcgatgccgg cgccgcactc
     1441 gaacactttc cagacgagct cggagcagta gatgcggtcg tccgaccact cgaaatacgg
     1501 gtcgtagtcc ttgccggcga gctgctcgcc gacggcgcgc atcttgcgca acgtatcgag
     1561 ggtcagccgc gcatccgcgt cgctcagccg cttagcgacg aaatgcccgc gctcgccgcg
     1621 ctcgatccac tcagtcaggg gagtcaactt gacgggctga acggcctcga agacgaacgg
     1681 ctgaccgtct cgcacataga cgatgcccat atggctgtac ggagactttg tcgcgagctg
     1741 aatcgccaag ctctgcgacg accgcgacgt ttggaacacg atgtcgccat cgtgcggctc
     1801 gtagcccgcg ttcggcgtgc aggcgcacag cgccaacaag ccgacgattc ccgcgcctct
     1861 caggtgcgga aaagcgcgca agctcacgca gggcgatgaa gagcgcgagt cgagctgccg
     1921 tcgttcgaca gccggctcaa cagccgccgg atgcgcgcgc cgatcgaagg gggtgccggc
     1981 ggcagcggcg ctgcggaatc ccattccgac atgatgcgat gcgcctcggc ggcgcagacg
     2041 tcgcaaatgt agccgcggcg gccggcgacg agcttcgcca cctgagcggc ggacttgccg
     2101 caaaacgagc acgtcagctt tcgtcttgga agcatcgaga ccctccagtc atttcggtgc
     2161 gtcacttgtc gtcacgcgag aaccgtgcgc ctcttcagcc gcagcatgta gagcaggacc
     2221 gcgacggcca ccggtaacgc ggaagacgat gtgacgcgca cgtcgaagta ggcctcgcgc
     2281 tcgggcgtca gcacgtacca caccgacgcg agcgccccaa gcgtgagata agccccgaac
     2341 caatacagcg agctcctgcg gaggcgaaag agcatcacca tcgacgtcag caggagcgcc
     2401 gaggccgcga acagcaagct ccaatcgagc gccgacagcg
//
LOCUS       DASSNN010000191         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_418641, whole genome shotgun sequence.
ACCESSION   DASSNN010000191 DASSNN010000000
VERSION     DASSNN010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418641"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..717
                     /locus_tag="VFL84_03470"
     CDS             124..717
                     /locus_tag="VFL84_03470"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET7607711.1"
                     /translation="MTEEEKRRPSTLLRLGVWALFVAAFAFLYYRYSSLFGTELTQLA
                     ASSVWLAYGLYVVLGALRGFALIPVTNLVVLAIPLFPPVPLLVLTLVGIAISSACIYA
                     FAGSLKLGEYLERKHARHMERLQNALRRNPTSIVTAWSFLPIVPTDLICYVCGAMNVS
                     FRRFMLGVLVGEGAICAIYIFAGASLLDLGMRLFGGS"
     gene            complement(714..2009)
                     /locus_tag="VFL84_03475"
     CDS             complement(714..2009)
                     /locus_tag="VFL84_03475"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HET7607712.1"
                     /translation="MRVREHGRLSGARVFEAAAAWLALCCALAGCSESPTTLRIVSGP
                     PDANERVAALLESASAEVEARVRLVRGPAVAGAEAALDALDHGSAELAIVENSASYRH
                     PDVRTVIPLYPSVLHIGVRPDKRAETLAQVLRGATVFAGDEQTPARQLLDRMKSIYAW
                     REIEFSYADSIDSRTDVIFTFAPITPSAAPLFDGFELFSLGDAAYVGTGSAADGLALV
                     APFLRSFVIPEGTYGPLTPAPIATVALDQLLVARADTSIVVVYDLIQSVQAIGPLLVA
                     QRPDLAIDELETFDISHVTFPVHAGTMAFRARNDPGFAERASSIFEMAVTIVVALVTA
                     LFALVRFVRGLRKGRIDKFYSATLDIRAKLAGERNPARRKAYVSRLRALRAEAFSLLI
                     KEKLAADDSFRILQALIYDVIREAEAPFEEIPAALEPSR"
BASE COUNT          338 a          746 c          718 g          387 t
ORIGIN      
        1 agcgcggctc ccacctgccg catgccgggg tcgttgttcg tgcccgtgcc gttcgtgcgc
       61 cactaacgcc gcgccggctc gcccttgcgc ccgatcgcga ttcgctgaca cgatcgccct
      121 cccatgaccg aggaagagaa gcgtcgcccg tccaccctgc tccgcctcgg cgtttgggcg
      181 ctgttcgtcg ctgcgttcgc gttcctctat taccgctatt cgtcgctatt cggcacggag
      241 ctgacgcaac tcgccgcgtc gtcggtctgg ctcgcgtacg ggctctatgt agtgcttggc
      301 gcattgcgcg gcttcgcgct gattcccgtc acgaacctcg tcgtgctcgc gattccgttg
      361 ttcccgcccg tgccgctgct cgtgctcacg ctcgtcggca tcgcaatctc gtctgcctgc
      421 atctatgcat tcgcgggctc gctgaagctc ggcgagtatc tcgagcgcaa gcatgcgcgc
      481 cacatggagc ggctgcagaa cgcgctgcgc cgcaacccga cctcgatcgt cacggcctgg
      541 agcttcctgc cgatcgtgcc gacggatctc atctgctacg tctgcggcgc catgaatgtc
      601 tcgtttcgcc gcttcatgct cggcgtgctc gtcggcgaag gcgcgatctg cgcgatctac
      661 attttcgcgg gcgcgtcctt actcgacctc ggcatgcgcc tgttcggcgg aagctagcgg
      721 ctcggctcga gcgcggccgg gatttcctcg aacggagcct cggcctcgcg gatgacgtcg
      781 tagatcaggg cttgaagaat acggaagctg tcgtctgctg cgagcttttc cttgatcagc
      841 agcgagaagg cctcggctcg cagtgcgcgt agccgcgaga cgtatgcctt gcgccgtgcc
      901 gggttgcgct cgccggcgag cttggcgcgg atatcgagcg ttgcagagta gaacttgtcg
      961 atgcggccct tacgaagccc ccgcacgaat cgcacgagcg cgaacagcgc cgtgaccagc
     1021 gcgacgacga tcgtcacggc catttcgaag atcgaagacg cgcgctccgc gaatcccgga
     1081 tcgttgcgcg cgcgaaacgc catcgtgccg gcgtggaccg ggaacgtgac gtgggagatg
     1141 tcgaacgtct cgagctcgtc gatcgcgaga tccggccgtt gtgcgacgag cagcgggccg
     1201 atcgcttgca ccgattgaat caagtcgtag accacgacga tcgacgtgtc cgcgcgcgcg
     1261 acgagcaact ggtcgagcgc aaccgtggcg atcggcgccg gcgtgagcgg cccgtaagtg
     1321 ccctccggga tcacgaacga ccgcaggaac ggcgcgacga gcgccagccc gtccgcggcg
     1381 gagccggtgc cgacgtaggc ggcgtcgccg aggctgaaca gctcgaaacc gtcgaacagc
     1441 ggcgcggcgc tcggcgtgat cggcgcgaac gtgaagatca cgtcggtacg gctatcgatc
     1501 gaatcggcat aggaaaactc gatctcgcgc cacgcataga tcgacttcat tcgatcgagc
     1561 agctgccgcg ccggcgtctg ctcgtcgccc gcgaataccg tggcgccgcg cagcacttgg
     1621 gcgagcgtct ccgcgcgctt gtcgggccgc actccgatgt gcagcacgct cgggtagagc
     1681 ggaatgacgg tccgcacgtc cggatgtcta tagctcgcgc tgttctcgac gatcgcgagc
     1741 tccgcgctgc cgtggtcgag cgcatcgagc gccgcttcgg cgcccgcgac agcgggaccg
     1801 cgcacgagac gaacccgcgc ttccacctcc gcgctggcgc tctcgagcag cgcggcaacg
     1861 cgctcgttcg cgtcgggcgg gccgctgacg atgcgcagcg tggtcggcga ttccgagcag
     1921 ccggcgagcg cgcagcacag agcgagccag gctgccgccg cctcgaagac gcgcgccccg
     1981 gatagtctgc cgtgctcacg tacgcgcatc gctgccccgg ctcagtgcat ccggtcgcgc
     2041 acgtcgatca gcatctcgtc ggccgcatcg acgcgaatga gcccgacgcg cgggaactcg
     2101 acgtcgacga tgacgtagat cgtcagcgag atgatcgcgg cgaacgtgac cgtgtgcagc
     2161 cagcttctcg tctgattctg cgacgtcga
//
LOCUS       DASSNN010000192         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_419034, whole genome shotgun sequence.
ACCESSION   DASSNN010000192 DASSNN010000000
VERSION     DASSNN010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419034"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFL84_03480"
     CDS             <1..651
                     /locus_tag="VFL84_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7607713.1"
                     /translation="TATMGEAPAGTTSAPGIWERAGNDTRWNPFMFPKLPGAIVADGE
                     GVRMPPGRTQIEWECELGVVVGRPASRVAQARAMDYVFGYTLENDMSDRGGRGDARFG
                     SDWLVAKNHDTFAPLGPFITPKEFVRDIGNAGMKFVLNGELLQEGTTAKMIHSIPEQI
                     VYASNIITLRPGDVIATGTPPGVGSARNPPILLKAGDRMECSYDGVGTLRNPVVAP"
     gene            832..2250
                     /locus_tag="VFL84_03485"
     CDS             832..2250
                     /locus_tag="VFL84_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HET7607714.1"
                     /translation="MACAAPFIPEEVVQHRGLEELEAEITELWGYLNAATYRFLVLVA
                     EFDRVRGYAHRGLANTAQWLNWQCGIGPVAAREKVRVARSLERLPEIADAFAKGEISY
                     SKVRAMTRVATPANESVLVSIARHGTASHLEKLVKRFEWTQRRDAASTAQRQHEHRNT
                     YCFYDMDGEFVLHARLPREIGAVVRKALDVAVEQVRESKEVTAETALQIPANAWTADA
                     LRLMCERFLEQRGEETGAGSSADRLQVVVHIDQAILAEQRTAAQDGEPHRCELEHGPA
                     LALDTARRLACDATVIGIVEGEDGEPLDIGRKTRSIPAAIARALRARDGGCRFPGCDR
                     TRFCDGHHVKHWADGGETKLANLITLCGFHHRLVHEGGYGVRRTDDGAFVFTRPDGTR
                     IEENGMKCFRGNSLPPIHSTYRGFEHSLRDYMKTRDPGLTITAQTSRCQWRGESMDYS
                     QAIEAMQFLESKAAVPERLPAG"
BASE COUNT          416 a          749 c          755 g          366 t
ORIGIN      
        1 acggcgacga tgggcgaagc cccggccggc acgacgagcg cgcccggcat ctgggagcgt
       61 gccggcaacg acacgcgctg gaacccgttc atgttcccta agctgcccgg cgcgatcgtc
      121 gccgacggcg aaggcgtgcg catgccgccg ggccgcacgc agatcgagtg ggagtgcgag
      181 ctcggcgtcg ttgtcggccg gccggcgtcg cgcgtcgcgc aagcgcgcgc gatggactac
      241 gtgttcggct atacgctcga gaacgacatg tcggaccgcg gcggccgcgg cgacgcgcgc
      301 ttcggctccg actggctcgt cgcgaagaac cacgacacgt tcgcgccgct cgggccgttc
      361 atcacgccga aggagttcgt ccgcgacatc ggcaacgccg gcatgaagtt cgtgctgaac
      421 ggtgagctgc tgcaggaagg cacgacggcg aagatgatcc actcgatccc cgagcagatc
      481 gtctatgcga gcaacatcat cacgctgcgt cccggcgacg tgatcgcgac gggcaccccg
      541 cccggcgtcg gctcagcgcg caatccgccg atcctgctca aggccggcga tcgcatggag
      601 tgcagctacg acggcgtagg cacgctgcgg aatcccgtcg tcgcgccgta gctcacgcgg
      661 gttccgatta cggcattagt tccaccgcaa gagctgcgct cgcgcgtcgg cccgttttcc
      721 tcttcgttgg aatggttctt cgcagtgacc gccggcctta actgattgac ttacggtcat
      781 tttcggccca caatactgta tatccaacca gccttaaaac cggaggtcga catggcctgc
      841 gccgctccct tcatccccga agaggtcgtt caacaccgcg ggctcgagga gctcgaagcg
      901 gagatcaccg agctctgggg ctacttgaac gccgcgactt accgctttct cgtgctcgtg
      961 gccgagttcg atcgcgtgcg aggctacgca catcgcggtc tcgccaacac ggcgcagtgg
     1021 ctgaactggc aatgcggcat cggcccggtg gctgctcgcg agaaggtccg cgtcgcgcgc
     1081 tcgctcgaac gcctgcccga gatcgccgat gcattcgcga aaggcgagat ttcgtactcg
     1141 aaggtccgcg cgatgacgcg cgtcgccact cccgcgaacg aatcggtgct cgtcagcatc
     1201 gcgcgtcacg gcacggcctc gcacctcgaa aagcttgtca aaagattcga gtggacgcag
     1261 cggcgcgatg ctgcgagcac cgcacagcgc cagcacgagc accgcaacac ctattgcttt
     1321 tacgacatgg atggggagtt cgtgcttcat gcgcggctac cgcgagaaat cggcgccgtc
     1381 gtgcgtaaag cgctcgacgt ggccgtcgag caggtgcggg agtcgaagga agtgacggcg
     1441 gagactgcgc tgcagattcc ggcgaatgcg tggaccgcgg atgcgctgcg gctcatgtgc
     1501 gagcgcttcc tcgagcagcg cggcgaggag accggagcgg gctcttcggc cgatcgactg
     1561 caagtcgtcg tgcacatcga tcaggcgatc ctcgcagagc aacgaaccgc ggcgcaagac
     1621 ggcgagccgc atcgctgcga gctcgagcat ggaccggcgc ttgcgctcga caccgcgcgg
     1681 cggcttgcgt gcgatgcgac cgtcatcggt atcgtcgaag gcgaagatgg cgagccgctc
     1741 gacatcggcc gcaagacgcg cagcatccct gctgcgatcg ccagagcgct tcgtgcgcgc
     1801 gacggcggtt gccgtttccc gggctgcgat cgcacgcgct tctgcgacgg gcatcacgtg
     1861 aagcattggg cggacggcgg cgaaacgaag ctcgctaact tgatcacgct ctgcggcttc
     1921 catcaccggc tcgtgcacga gggaggttac ggggtcaggc gcaccgacga cggagccttc
     1981 gtcttcacgc ggccggacgg cacgcgcatc gaggagaacg gcatgaaatg tttccgcgga
     2041 aacagtttgc cgccgatcca ttcgacctat cgcggcttcg agcacagtct gcgcgactac
     2101 atgaagacgc gcgatcccgg cctcacgatc accgctcaga cgagccgctg ccagtggcgc
     2161 ggcgagagca tggactacag tcaggccatc gaggcgatgc aattcctcga gagcaaagcc
     2221 gcagtcccgg aacgattacc ggccggctag tcggcggagc ccagcgccac gaagacattc
     2281 cggagc
//
LOCUS       DASSNN010000193         4076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_905467, whole genome shotgun sequence.
ACCESSION   DASSNN010000193 DASSNN010000000
VERSION     DASSNN010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905467"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFL84_03490"
     CDS             <1..465
                     /locus_tag="VFL84_03490"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET7607715.1"
                     /translation="IDGEIIDVAPPGSPHAGTVDQLALVLGRAVVGRAHVRVQNPLRL
                     DEHSEPQPDISVLKLSADFYKSRHPRASDALLVVEVADTSLRYDRDDKIPLYARHLIP
                     EVWLVDLKAKCLVRYRNPQQGAYASVDHPDLGSALEIAALPETRVDLSALFS"
     gene            complement(476..916)
                     /gene="rnhA"
                     /locus_tag="VFL84_03495"
     CDS             complement(476..916)
                     /gene="rnhA"
                     /locus_tag="VFL84_03495"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HET7607716.1"
                     /translation="MPNVEAFTDGACRGNPGPGGWGVVLRSGHRVKELFGGDPATTNN
                     RMELRAAIEALAALKQPCKVDLYTDSVYVRSGITEWLPAWRARGWRTADKKPVKNQDL
                     WEALAALAAKHDVTWHWVKGHSGHPENERADELANEGIDAMLSS"
     gene            complement(916..1683)
                     /locus_tag="VFL84_03500"
     CDS             complement(916..1683)
                     /locus_tag="VFL84_03500"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7607717.1"
                     /translation="MSSEPAEVKEWLSTPLGRRCLANEQRLMRRALERVFGEQMLQIG
                     AWGPRNSFLRYARTQRRALVDWRPGVAADLVSNPEQLAIASDSIDAVLLPHTLERTSS
                     PHAVLREVDRVLRADGHLIVLSFASGGLWGLRHLLSSGGYPNGRDQAIREGRLRDWLE
                     LLSFEVPAATRYCHTWPIERFSQIKILPREEWSQRWLPLFSGGYLLRAQKRVHPLTPV
                     RLWRRQPRLRVVGGLVEPTTRAAARALPHPGGEQSEP"
     gene            1816..3486
                     /locus_tag="VFL84_03505"
     CDS             1816..3486
                     /locus_tag="VFL84_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7607718.1"
                     /translation="MDNNNDGLRRSSRPLAVLALSAVVSACASTGPELEAPPPPPAPV
                     AAVALEPIVLPALKYRDVVVEPDLLTRLRSRFEWQIKDDAAVERERAWYAKNQAYLDR
                     VFKRADLYLYHIVGELEARGMPAELALLPVVESAFDPFAYSHGRASGLWQIIPGTGKR
                     LGLAQNWWFDGRRDVLESTRAALDYLEDLHRQFDGDWLLAVAGYNSGEGNVARALKKA
                     AAAGKPQDFWGIKGYLPPETRTYVPRLLAIAEVVGQPEAFGVTLPKLTNEPRFAVVET
                     GGQIDMALAAELAGIETAALYALNAGVNRWATDPDGPHRLLLPIEQAPEFTTALAALG
                     PRERVQWTRHKVKAGETVGGIAERYQTTPAVLRQVNGFRGNTIRAGDYLMIPTATQSL
                     ASYTQSADSRAARQQGAVRNGERRTHVVRSGESLWSIAQAYGVGMRNLASWNAMAPGD
                     TLGVGRELVIWTSAAAAEAAASSPAPVTTTAQASLVSTSSSAGFAATNRMREITYVVR
                     RGDSLSSIARRFRVTVDKLVEWNAGAAGKYLQPGQRLKMVVDITAQSG"
     gene            3486..>4076
                     /locus_tag="VFL84_03510"
     CDS             3486..>4076
                     /locus_tag="VFL84_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HET7607719.1"
                     /translation="MGFLAGKRAVIVGLASNRSIAWGIAQAMRREGAELAFGYQNDKL
                     KKRVEDMAHELGSDITLPLDVAEDAQIDAFFEGIAKRWDGFDILVHSVAFAPAEQLEG
                     RYLDAVTREGFRIAHDISSYSLAALARAFQPMMEGRNSAILTLTYLGAVRALPSYNVM
                     GLAKASLEANVRFLALDLGPGNIRVNAISAGPIRTLA"
BASE COUNT          636 a         1446 c         1383 g          611 t
ORIGIN      
        1 atcgacggcg agatcatcga cgtggcgccg cctggatccc cgcacgccgg caccgtggat
       61 cagctcgcgc tggttcttgg ccgcgccgtc gtggggcgcg cgcacgtgcg tgttcagaac
      121 cctctaaggc tcgacgagca ctcggagccg caaccggata tttcggtcct gaagttaagc
      181 gccgacttct acaagtctcg tcaccctcgc gcttcggatg cgttgctcgt cgtcgaagtg
      241 gccgatacct cactgcgcta cgaccgcgac gataagatcc cgctgtacgc gcgccatctg
      301 attcccgaag tttggctcgt cgatctcaag gccaagtgcc tcgtccgcta tcgcaacccg
      361 cagcaaggcg cgtacgcgtc ggtcgaccac cccgatctcg gctctgcgct cgagatcgcc
      421 gccctccccg aaacgcgcgt cgacctgagc gcgctgttca gctgatcggc gcagatcagg
      481 acgaaagcat cgcgtcgatg ccttcgttcg cgagctcgtc cgcgcgctcg ttctcgggat
      541 ggcccgagtg gcccttgacc cagtgccagg tgacgtcgtg cttcgccgcg agcgcggcga
      601 gcgcctccca gagatcctga ttcttgacgg gcttcttgtc cgcggtgcgc cagccgcgcg
      661 cgcgccacgc gggcagccac tccgtgatgc cgctgcgcac gtagacggaa tcggtgtaga
      721 gatcgacctt gcacggctgc ttgagcgcgg ccagcgcctc gatcgcggcg cgcagctcca
      781 tgcggttgtt cgtcgtcgcg ggatcgccgc cgaagagctc cttgacgcga tgccccgagc
      841 gcagcacgac gccccagccc cccggccccg ggttgccgcg gcaagcgccg tcggtgaacg
      901 cttcgacgtt cggcactagg gctcggactg ctcgccgccc ggatgcggca gagcgcgcgc
      961 ggccgcgcgc gtcgtcggct cgacgagccc gccgaccacg cgcaagcgcg gctgacggcg
     1021 ccacaaccgc acgggcgtga gcgggtgcac gcgcttctgc gcgcgcaaga gataaccgcc
     1081 gctgaagagg ggcagccagc gctgcgacca ctcctcgcgc ggcaggatct tgatctggct
     1141 gaaccgctcg atcggccacg tgtggcagta gcgcgtcgcg gcgggcacct cgaacgacaa
     1201 gagctcgagc cagtcgcgca ggcgcccttc gcgaatcgcc tgatcgcggc cgttcggata
     1261 accgccgctc gagagcaagt gccgcaggcc ccagagcccg cccgacgcga agctcaagac
     1321 gatcaggtgg ccatcggcgc gcaagacgcg gtcgacttcg cgcagcacgg cgtgcggcga
     1381 cgaggtgcgc tcgagcgtgt gcggcagcag cacggcgtcg atcgaatcgc tcgcgatggc
     1441 gagctgctcg ggattgctga ccaagtccgc ggccacgccg ggccgccagt cgacgagcgc
     1501 gcgccgctgc gtgcgcgcgt agcgcaggaa cgagttgcgc gggccccagg cgccgatctg
     1561 cagcatctgc tcgccaaaga cgcgctccaa ggcgcggcgc atgagccgct gctcattggc
     1621 aaggcaccgc cgcccgagcg gcgtcgagag ccattccttg acctcggccg gttcgctgct
     1681 catgggtcga tcctgcctga gagggccatc acagttcaat gcggtagtac gcgtcgccag
     1741 acggacacac cttgcttgac cgtcgatcct cggtaagcta cggccatcac ctagcaaaat
     1801 gggctccggc gctctatgga caacaataac gacgggttgc gccggagcag ccgccctctc
     1861 gccgtcctcg cgctctccgc cgtcgtttcc gcctgcgcat ccacgggccc cgagctcgag
     1921 gccccgcccc cgccgcccgc ccccgtcgcc gccgtcgcgc tcgagccgat cgtgctgccc
     1981 gcgctgaaat accgcgacgt cgtcgtcgag ccggacctgc tgacgcgcct gcggtcgcgc
     2041 ttcgagtggc agatcaagga cgacgcggcc gtcgagcgcg agcgcgcctg gtacgcgaag
     2101 aatcaggcct atctcgaccg cgtcttcaag cgcgcggact tgtacctcta ccacatcgtc
     2161 ggcgagctcg aggcgcgcgg catgccggcc gagctcgcac tgttaccggt cgtcgagagc
     2221 gcattcgatc cgttcgcgta ctcgcacggc cgcgcgtcgg gcttgtggca gatcatcccc
     2281 ggcacgggca agcgcctagg gctcgcgcag aactggtggt tcgacggccg ccgcgacgtg
     2341 ctcgagtcga cgcgcgccgc gctcgactat ctcgaggacc tgcaccggca gttcgacggc
     2401 gattggctgc tcgccgtcgc cggctacaac tccggcgaag gcaacgtggc gcgcgcgctg
     2461 aagaaggccg cggccgcggg caagccgcag gatttctggg gcatcaaggg ctatttgccg
     2521 cccgagacgc ggacctacgt gccgcgcctg ctcgcgatcg ccgaggtcgt cggccagccc
     2581 gaagcgttcg gcgtcacgct gcctaagctt acgaacgagc cgcgcttcgc cgtcgtcgag
     2641 acgggcggcc agatcgacat ggcgctcgcg gccgagctcg cgggcatcga gaccgccgcg
     2701 ctgtacgcgc tgaacgccgg ggtcaatcgc tgggcgaccg atcccgacgg gccgcatcgg
     2761 ctgctgctac cgatcgagca agcgccggag ttcacgacgg cgctcgcggc gcttgggcca
     2821 cgcgagcgcg tgcaatggac gcggcacaag gtcaaagccg gcgagaccgt cggcggcatc
     2881 gccgagcgct atcagacgac gccggccgtg ctgcgccagg tcaacggctt tcgcggcaac
     2941 acgatccgcg ccggcgacta cttgatgatt ccgacggcga cgcagtcgct cgcgagctat
     3001 acgcagagcg cggactcgcg cgcggctcgc cagcaaggcg ccgtgcgcaa cggcgagcgg
     3061 cgcacgcacg tcgtgcggtc gggcgagtcg ctgtggtcga tcgcgcaggc gtacggcgtc
     3121 ggcatgcgca acctcgcgag ctggaacgcg atggcgcccg gggacacgct cggcgtcggc
     3181 cgcgagctcg tcatctggac gagcgcggcg gccgcggaag cggcggcttc gtcgccggcg
     3241 ccggtgacga cgacggcgca agcctcgctc gtcagcacga gctcgtcggc cggcttcgcg
     3301 gcgacgaacc gcatgcgcga gatcacctac gtcgtgcgcc gcggcgattc gctgtcgtcg
     3361 atcgcgcggc ggttcagggt cacggtcgac aagctcgtcg agtggaacgc cggcgcggcc
     3421 ggcaagtact tgcagccggg ccagcgcctc aagatggtcg tcgacatcac ggcgcagagc
     3481 ggctgatggg cttcctcgca ggcaaacggg ccgtcatcgt cgggctcgcg agcaatcgct
     3541 cgatcgcgtg gggcatcgcg caagcgatgc gccgcgaagg cgccgagctc gcgttcggct
     3601 atcagaacga caagctcaag aagcgcgtcg aggacatggc tcatgagctc ggcagcgaca
     3661 tcacgctgcc gctcgacgtc gccgaggatg cgcagatcga cgcgttcttc gaaggtatcg
     3721 cgaagcgctg ggacgggttc gacatcctcg tgcactccgt cgcgttcgcg ccggccgagc
     3781 agctcgaagg ccgctatctc gacgccgtga cgcgcgaggg gttccgcatc gcgcacgaca
     3841 tctcgagcta cagtctcgcg gcgctcgccc gggcgttcca gccgatgatg gaaggcagga
     3901 actcggcgat cctcacgctc acgtacctcg gcgccgtgcg cgcgctgccg agctacaacg
     3961 tcatggggct cgccaaggcc agcctcgagg ccaacgtgcg ctttctggcg ctcgacctcg
     4021 gcccgggcaa tatccgcgtc aatgccatct cggcgggccc gatccgcacg ttggcc
//
LOCUS       DASSNN010000194         4404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1660391, whole genome shotgun sequence.
ACCESSION   DASSNN010000194 DASSNN010000000
VERSION     DASSNN010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660391"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFL84_03515"
     CDS             <1..267
                     /locus_tag="VFL84_03515"
                     /EC_number="5.99.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="decatenates newly replicated chromosomal DNA and
                     relaxes positive and negative DNA supercoiling; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET7607720.1"
                     /translation="RLLVFALQELPELSKGKGNKILSVPSKAGVTLAGICVLAPGQGL
                     RIDSGTRHMTIKAADLEHYAGERAHRGMALPRGWRTVDRLSVEP"
     gene            complement(422..2449)
                     /locus_tag="VFL84_03520"
     CDS             complement(422..2449)
                     /locus_tag="VFL84_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7607721.1"
                     /translation="MLSGREDDVELVNRSLRDGGHAVRCHWVAKVDKLLGALQEHAPE
                     LLCFFPDSLPIAIREIAKIKQHAAGGVPLVVVSKDAQETDIAEALLAGAQDLVSLGQT
                     DRLRSVAERELRSYRLERALNDTLFSANQYKKQLKAFMAGSVDAIAYVQEGILIDANQ
                     AWADLLGHASAEALFGTPLMDSFDGASKAALKGALVACGKGQWNRESIKIVATTRSGD
                     KLPLEIQLEVASVDGDPAVKLSVPRPRVETREPEEIIEQAVHKDPATGFYHRRRFLEV
                     LTDRLDQKGAGVRVLAYIRPDKFRELEEQIGPLATEDVLVQIAEQLRDLLQPNDLAGR
                     FGGQVFTLFLERGTLRDVEAWAQSALARIAGRIFESAHNTLSVTCTIGLAEVGPTTER
                     LDALIAQARRSNQAGRDQGGNRVVLEQTSDESTLVQRFDEIWVQQIKTALMENRFRLA
                     HLPIANLSGELRSMYDTVLRMIDAQGDEVAASEFLPAAARNRLLRAIDRWVIGASLSF
                     CAKTPLECVFIKLSSESIIDKTLIEWLTKAVASSGVAARHLCFQISEENATQYLAQTQ
                     ALAAQLRSMGHRFAIENFGVGRDSGRILTQTPMDFLKIDGSLMQSIASDQTRQEKVRV
                     YVAAAQNRAIPVIAERVEDANTMAVLFQLGVAYMQGHYVHEPEVVLADAGR"
     gene            complement(2561..3607)
                     /gene="epmB"
                     /locus_tag="VFL84_03525"
     CDS             complement(2561..3607)
                     /gene="epmB"
                     /locus_tag="VFL84_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011507825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HET7607722.1"
                     /translation="MRMGNDATSSIRTADLSAADPAAAALPLPSWQAELRNAVRDPAE
                     LERLLELTPGALGPPAGTAFPLLVPRGFVARMRKRDTSDPLLKQVWPAAAELEAVEGF
                     SADPVREQGLAGHGLIKKYFGRVLLIASGACPLHCRYCFRREFPYSAQLAARADWGPA
                     IAALRGVTDAKEAILSGGDPLSLSNLRLGELLARIADTAVTTVRIHTRFPIAIPERID
                     AGLLRVLRGTRLETVVVVHTNHANELDAGVERALKAMRPEITALLNQSVLLKGVNDDA
                     ETLADLSERLFACGVLPYYLHLLDRVAGAAHFDVDAARGKALVAALRARLPGYLVPRL
                     VRETPGELSKTVVA"
     gene            3698..4267
                     /gene="efp"
                     /locus_tag="VFL84_03530"
     CDS             3698..4267
                     /gene="efp"
                     /locus_tag="VFL84_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET7607723.1"
                     /translation="MAVYSTNEFKSGLKILLDGDPYSILENEYVKPGKGQAFNRVKVR
                     NLKTGRVVEKTFKSGDTAEGADVVDQDMQYLYNDGQFWYFMQPDTFEQLAAGEAAVAD
                     AKDWIKEQQSCIVTLWDGVPLYVAAPNHVELKIVETDPGVRGDTATGGQKPAKLETGA
                     VVRVPLFINEGEVIRVDTRTGTYISRVKQ"
BASE COUNT          740 a         1454 c         1480 g          730 t
ORIGIN      
        1 cggctgctcg tgttcgcgct tcaggagctg cccgagctca gcaaaggcaa gggcaacaag
       61 atcctctcgg tgccgagcaa ggcgggcgtc acgctcgccg gcatttgcgt gctcgcaccg
      121 gggcaaggct tacgcatcga ctcgggcacg cgccacatga cgatcaaggc cgccgacctc
      181 gagcactacg cgggcgagcg cgcgcaccgt ggcatggcgc tgccgcgcgg ctggcgaacc
      241 gtggatcggc tcagcgtcga gccgtaaaag gggacagatc tatttacgcg cccgaaaacg
      301 ccgtaaaagg ggacagatct atttacgcgc ccgaaaatgg atctgtatcc cgtgacatct
      361 acgcggcgcg taaatagatc tgtccccttt tacggcggag aaaaggggtc tgtccccttt
      421 tctagcggcc ggcgtcggcg aggacgactt cgggctcgtg cacgtagtga ccttgcatgt
      481 aggcgacgcc gagctggaat agcacggcca tcgtattggc gtcctcgacg cgttcggcga
      541 tgaccgggat cgcgcggttc tgcgcggcgg cgacgtagac gcgcactttc tcctggcgcg
      601 tctggtcgct cgcgatgctc tgcatcagcg agccgtcgat cttcaggaag tccatcggcg
      661 tctgcgtcag gattcggccc gagtcgcggc ccactccgaa gttctcgatc gcgaagcgat
      721 ggcccatgga gcgcagctgc gcggccagcg cttgcgtctg cgcgaggtac tgcgtcgcgt
      781 tctcctcgct gatctggaag cacaaatgcc gtgcggcgac gccggagctc gcgacggcct
      841 tcgtgagcca ctcgatcagc gtcttgtcga tgatcgactc cgacgacagc ttgatgaaca
      901 cgcactcgag cggggtcttt gcgcagaacg acaggctcgc gccgatgacc cagcggtcga
      961 tcgcgcgcag caggcgattg cgcgccgcgg ccggcaggaa ctcgctggcc gcgacctcgt
     1021 cgccttgggc gtcgatcatg cgcagcacgg tgtcgtacat cgagcgcagc tcgccgctca
     1081 agttcgcgat cggcagatgc gcgagcctga accggttctc catgagcgcg gtcttgatct
     1141 gctgcaccca gatctcgtcg aagcgctgca cgagcgtgct ttcgtcggag gtctgctcga
     1201 gcacgaccct gttgccgcct tgatcgcggc cggcttggtt cgagcggcgc gcttgcgcga
     1261 tcagcgcgtc gagccgctcc gtcgtcggcc cgacctcggc caagccgatc gtgcatgtaa
     1321 ccgacagcgt gttgtgcgcg ctttcgaaga tgcggcccgc gatgcgcgcg agcgcgctct
     1381 gcgcccaggc ctcgacgtca cgcagcgtgc cgcgctccag gaacagcgtg aacacctggc
     1441 cgccgaagcg gccggcgagg tcgttcggct gcagcaggtc gcgcagctgc tcggcgatct
     1501 gcacgaggac gtcctcggtc gcgagcggcc cgatctgctc ctcgagctcg cggaacttgt
     1561 cgggccggat gtacgcgagc acgcgcacgc cggcaccctt ctggtcgagc cgatccgtca
     1621 acacctcgag gaagcgccgc cggtgataga agcccgtcgc cgggtccttg tggaccgcct
     1681 gctcgatgat ctcctcgggc tcgcgcgtct cgacgcgcgg ccgcggcacg ctgagcttga
     1741 cggccggatc gccgtcgacc gacgcgacct cgagctggat ctcgagcggc agcttgtcgc
     1801 cgctgcgcgt cgtggcgacg atcttgatcg actcgcgatt ccactggcct ttgccgcacg
     1861 cgacgagcgc gcccttcagc gcggccttgc tcgcgccgtc gaacgagtcc atgagcggcg
     1921 tgccgaaaag cgcctcggcg ctcgcgtggc cgagcaagtc ggcccacgcc tggttcgcgt
     1981 cgatcaggat tccttcttga acgtacgcga tggcgtcgac ggagcccgcc atgaacgcct
     2041 tgagctgctt cttgtattga ttcgccgaga acagcgtgtc gttgagcgcg cgctcgagcc
     2101 tataggagcg cagctcgcgc tcggcgacgc tgcgcaagcg atcggtctgg ccgagcgaga
     2161 cgagatcctg cgcgcccgcg agcagcgcct cggcgatgtc ggtttcctgc gcgtccttgc
     2221 tgacgacgac gagcggcacg ccgcccgcgg cgtgctgctt gatcttggcg atctcgcgga
     2281 tcgcgatcgg cagactgtcg gggaagaagc agaggagctc cggcgcatgc tcctgcagcg
     2341 cgccgagcag cttgtcgact ttcgcgaccc agtggcagcg caccgcatgc ccgccgtcgc
     2401 gcaacgaacg gttgacgagc tcgacgtcgt cttcgcgtcc gctgagcacg attagcggta
     2461 ccggggcttg caccatgccg tcgttgttct agtggcgaag actggcagtg taagcagtgc
     2521 cgcacggccc catcgggacg ccggtcacag tcacggaacc ctaggcgacg acggttttac
     2581 ttaactcccc gggggtctcg cgaacgagcc tcggaacgag gtatccgggc aagcgggccc
     2641 gcagcgccgc gacgagcgct ttgcctcgcg ccgcgtcgac gtcgaagtgc gcggcacccg
     2701 cgacgcggtc gagcaggtgc aggtaatagg gcagcacgcc gcacgcgaac aagcgctcgc
     2761 tcaaatcggc gagcgtctcg gcatcgtcgt tgacgccttt gagcaacacg gattgattca
     2821 acagcgccgt gatctcgggt cgcatcgcct tgagcgcgcg ctcgacgccc gcgtcgagct
     2881 cgttcgcgtg attcgtgtgg acgacgacga cggtttcgag ccgcgtgccg cgcaggacgc
     2941 ggagcagacc cgcgtcgatg cgctccggta tcgcgatcgg aaagcgcgta tggatgcgga
     3001 ccgtcgtgac cgcagtgtcg gcgatacgcg cgagcagctc gccgagccta aggttcgaca
     3061 gcgacagcgg atcgccgccg ctcaagatcg cttccttcgc gtcggtgacg ccgcgcagcg
     3121 ccgcgatcgc ggggccccag tccgcgcgcg ccgcgagttg cgcggagtaa gggaactcgc
     3181 gccggaaaca gtagcggcag tgcagcgggc aggcgccgct cgcgatcagc agcacgcgcc
     3241 cgaagtactt cttgatgagc ccgtggcccg cgagcccttg ctcgcgcacg ggatcggccg
     3301 agaagccctc gacggcctcg agctcggcgg ccgcgggcca cacctgcttg agcagcggat
     3361 cgctcgtgtc gcgcttgcgc atgcgagcga cgaagccgcg cggcacgagc agcgggaacg
     3421 cggtgccggc cggcggcccc agcgcgccgg gcgtgagctc gaggagccgc tcgagctcgg
     3481 ccggatcgcg cacggcgttg cgtagctcgg cttgccatga cggcagcggc agtgccgccg
     3541 cggccgggtc ggctgccgac agatctgccg tgcggatcga tgacgtggcg tcgttgccca
     3601 tgcgcataaa aagaggagcg gcctcagcga gttcgtgttc cgcggagctt gagagtatga
     3661 tcccgcgctc gctttttgat cctgagattc aactcgcatg gcagtttaca gcacgaacga
     3721 attcaagagc gggctcaaga tcctgctcga tggcgacccg tactcgatcc tcgagaacga
     3781 gtacgtcaag cccggcaagg ggcaggcgtt caatcgcgtc aaggtccgca atctgaagac
     3841 cgggcgcgtc gtcgagaaga ccttcaagtc cggcgatacg gccgaaggcg ccgacgtcgt
     3901 cgaccaggac atgcagtatc tctacaacga cggccagttc tggtacttca tgcagcccga
     3961 cacgttcgag cagctcgccg ccggcgaggc cgcggtcgcc gacgcgaaag actggatcaa
     4021 ggagcagcag agctgcatcg tcacgttgtg ggacggcgtg ccgctctacg tcgcggcgcc
     4081 gaatcacgtc gagctcaaga tcgtcgagac cgatcccggc gtgcgcggcg acacggcgac
     4141 cggcggtcag aagcccgcga agctcgagac gggcgcggtc gtgcgcgtgc cgttgttcat
     4201 caacgagggc gaggtcatcc gcgtcgacac gcggaccggc acctacatct cgcgcgtcaa
     4261 gcaataaccg acggcaataa ccaacggtcc caaccaacgg tgcctcaacc aacggtgccg
     4321 gtgttacggt tcggttcaac cgaaccaacg gtgccggtgt taccgttcgg ttcaaccgaa
     4381 caccggtgct tcggttcaac cgaa
//
LOCUS       DASSNN010000195         2929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1786876, whole genome shotgun sequence.
ACCESSION   DASSNN010000195 DASSNN010000000
VERSION     DASSNN010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786876"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VFL84_03535"
     CDS             complement(<1..1104)
                     /locus_tag="VFL84_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607724.1"
                     /translation="MRSGIYRTALLRYREAAAAGLDSGLLHYNLGVVHYELEDFTAAA
                     DEFARAAADPALAALASLNRGLALEASGNAAAASDAFRAAADAADDRRLRRAAENAAA
                     NSVAASAPPASRSRTFESIAEPTARIGRLELSAAARVGQDGNVYRTPADPYVDLSDPT
                     QPLVTPVVHSASFMPAELHALYALDNESGDTQFEFRYDMDGAFYDAEFSDANVVDQSV
                     SMGAAIVLGDRQGRRECRECRTQFAAERRRAVDTAFFVGTHSETNFDPDDGLPRDIVV
                     LVNGQPVVEDLSERFSYKAAGVNGELTHTLGRVTWRLNLKFERDEYERTEAVANFDHD
                     FFYGGVEVDYDLSAAMMLRFGLRKYRTVYDERPA"
     gene            complement(1298..1774)
                     /locus_tag="VFL84_03540"
     CDS             complement(1298..1774)
                     /locus_tag="VFL84_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607725.1"
                     /translation="MTVPPTDSKPSAAAPAAQRPKPTVAQRVVGFAFIGAFGLTMLTV
                     IMTGLYVRSPNAPRSPENPAVTLTVGETRTINLVFGSRVEVDDVEITVDLPPGIELKS
                     HPGQHRVTAHATLAGGDSAVPLTLVARGGSGGQIAARLTHGGEQKVFVVDVTVVEP"
     gene            1971..2777
                     /locus_tag="VFL84_03545"
     CDS             1971..2777
                     /locus_tag="VFL84_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET7607726.1"
                     /translation="MRAAATIALAALAFGTASGDAAARSLQDVLNTGTLRVGVTLFAP
                     WAARGKDGELVGFEVDVAHQLAADMGLKAQIFSYDIDRLVPALEAGDIDVIAAGLSIT
                     PERALHVNFSNPYAESGIGLATHVERTASVTDVRALDSDTITIAAVEGSVGVELARRL
                     WPRARVQTFQSVEAASRALVAGEVHGYVEDEPVPTFLALEHPGVVDVPTGRPLLASPA
                     GFAVNKGDPDFLAFLNAWITARNADTWLPTTASYWFESLKWRERLGDARR"
BASE COUNT          455 a         1015 c         1019 g          440 t
ORIGIN      
        1 cgccggccgc tcgtcgtaga ccgtacggta cttgcgcagg ccgaagcgca gcatcatcgc
       61 ggcgctcaag tcgtagtcga cctcgacgcc cccatagaag aagtcgtggt cgaaattcgc
      121 gaccgcctcg gtacgctcgt actcgtcccg ctcgaacttt agattcaagc gccacgtcac
      181 gcggcccagc gtgtgcgtga gctcgccgtt cactccggcg gctttgtacg agaacctttc
      241 gctcaagtcc tcgacgacgg gctggccgtt gaccagcacg acgatgtcgc gcggcaagcc
      301 gtcgtcggga tcgaagttcg tctcgctgtg cgtcccgacg aaaaacgcgg tgtcgactgc
      361 gcggcggcgc tcggccgcga attgcgtcct gcattcgcgg cactcccgcc gtccctggcg
      421 gtcgccgagc acgatggcgg cgcccatcga gacgctctga tcgacgacgt tcgcgtcgga
      481 gaattccgcg tcgtagaacg cgccgtccat gtcgtagcga aactcgaact gcgtgtcgcc
      541 cgattcgttg tcgagcgcgt agagcgcgtg cagctccgcg ggcatgaagc tcgcggaatg
      601 cacgacgggc gtcacgagcg gctgcgtcgg atcggacagg tccacgtaag gatcggccgg
      661 cgtgcggtag acattgccgt cttgacctac gcgcgccgcg gccgagagct cgagccggcc
      721 gatccgcgcg gtcggctcgg cgatgctctc gaaggtgcgg ctgcggctcg ccggcggcgc
      781 gctcgcggcg acggaattgg cggccgcgtt ctcggctgcc cgccgcaagc ggcggtcgtc
      841 ggcggcgtcg gccgccgcgc gaaacgcgtc gctcgccgcg gccgcgtttc cagaagcctc
      901 gagcgccagg ccgcggttca agctcgcgag cgcggccagc gccgggtccg cggcggcgcg
      961 cgcgaactcg tcggccgctg ccgtgaaatc ctcgagctcg taatgcacga cgcccaagtt
     1021 gtagtgcagg agaccgctgt cgaggcccgc ggcggccgcc tcgcggtacc gcagcaacgc
     1081 ggtgcggtag atccccgacc tcacgagttg attgccctcg gccagcagct cggcgccgct
     1141 ctgggccgcc gcctgggtgc cggagcaagc cgttaaagcg agcgcgatca aagacttgga
     1201 atggcgcacg gcgcgtccct tcgaggaagc ttgcgccact ctaggcgggc cgcgacggcc
     1261 cgaataggac ggccgtcaca cggagttacg cagagattta cggctcgacg acggtgacgt
     1321 cgacgacgaa caccttctgc tcgccgccgt gggtcagacg cgctgcgatc tgaccgccgc
     1381 tgccgccgcg cgcgacgagc gtcagcggca ccgcgctgtc gccgccggcg agcgtagcgt
     1441 gtgccgtcac gcggtgctgc ccgggatgcg acttcagctc gatgcccggc ggcagatcga
     1501 ccgtgatctc gacgtcgtcg acctcgacgc gcgagccgaa cacgagattg atcgtacgcg
     1561 tctcgccgac ggtcaaggtc acggccgggt tctccggcga gcgcggcgca ttcgggcttc
     1621 gcacgtacag gcccgtcatg atgacggtca gcatcgtgag accgaacgcg ccgatgaaag
     1681 cgaacccgac gacgcgttgc gcaaccgtcg gcttcggacg ttgcgcggct ggagccgccg
     1741 cggaaggttt cgaatcggtc ggcggcacgg tcacgggcgc acgaccgcca aagggccggg
     1801 gcctcgatcg gcttcgcagg ctcgttcggc taccattctc gcggaaccac gcacgctcgg
     1861 gatcataccc gagcgcgcgc cgtccttacc gagcggacgt aaggcctagc gcgggccgaa
     1921 cctaaggacg gcggccaggt ctcaagtttg cgtaactcga ggtagatagc atgcgcgcgg
     1981 cagcgacgat cgcattggcg gccttggcgt tcggaaccgc gagcggcgac gccgccgcgc
     2041 gaagcctgca ggacgtgctc aacaccggca cgctgcgcgt cggagtgact ttgttcgcgc
     2101 cgtgggccgc gcgcggcaag gacggagagc tcgtcggctt cgaggtcgac gtcgcgcatc
     2161 agctcgccgc cgacatgggg ctgaaagccc agatcttttc ctacgacatc gaccgcctcg
     2221 tgccggcgct cgaagcgggc gacatcgacg tcatcgccgc ggggctctcg atcacgcccg
     2281 agcgcgcgct gcacgtgaac ttcagcaatc cgtacgcgga gagcggcatc ggcctcgcga
     2341 cgcacgtcga gcgcacggcg agcgtcaccg acgtcagggc gctcgacagc gatacgatca
     2401 cgatcgccgc ggtcgagggc tccgtcggcg tggagctcgc gcggcggctg tggccgcgcg
     2461 ctcgagtgca aaccttccaa tccgtcgaag cggcgagccg cgcgctcgtc gccggcgagg
     2521 tccacggcta cgtcgaggac gagcccgtgc caacgttctt ggcgctcgag catcccggag
     2581 tcgtagacgt gccgaccggg cgcccactgc tcgcgagccc ggccggcttc gccgtcaaca
     2641 agggagaccc cgacttcctc gccttcttga atgcgtggat cacggcgcgc aacgccgaca
     2701 cgtggctccc gaccacggcg agctactggt tcgagtcgtt gaaatggcga gagcggctcg
     2761 gcgatgcgcg gcgctaagcg acaaatcgcg gtcctgcttg cggcgctagg ggctgtcgcc
     2821 gcgtggccgc tcgcggcgca gaacgccgag ccgccgcgcg cgccgcgaat cctcgacgtg
     2881 ccgcgggcgt ccttgcccga ggcggccgcg ccgacgccgg ccgtgcagc
//
LOCUS       DASSNN010000196         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1786902, whole genome shotgun sequence.
ACCESSION   DASSNN010000196 DASSNN010000000
VERSION     DASSNN010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786902"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VFL84_03550"
     CDS             complement(<1..900)
                     /locus_tag="VFL84_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607727.1"
                     /translation="MHEWIRALRRLTRRPASAAAIVITVALGIGISAGMFSVLHGVVL
                     RALPYPDADRLVRIYTGAPSRGPGRGGLTRAEVLEGLPSLPGFESTAYYINEPPYLFN
                     GAGLARRVGVVRVSADFFTVFGVPAALGRTFIASDFGETGPVAVLSHEAWTELTGGDP
                     SAVGRTLTFTQASVEVVGVLPPSFTESTGEALVYVPFSASDLGAPRFLYGQRQIVAVG
                     RLAAGTSRAAARNALATRMAGVATDGRPNDFQYAFVGLLDELVAGVRVVLSGLFMVSL
                     LVLLIACATAASLVAIRFHARGAE"
     gene            complement(938..1651)
                     /locus_tag="VFL84_03555"
     CDS             complement(938..1651)
                     /locus_tag="VFL84_03555"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HET7607728.1"
                     /translation="MIQGAVNERLIPVRQTWAAILALVLASMLSCALVVVRAYYAGTT
                     DYLFFFWNLFLAWVPLGAALSFYGLAARQSRNWVALLAVAVIWFLFFPNAPYIVTDLV
                     HLREARPVPYWYDIICVMAFAQTGLFLGYLSLYLMQEVVRAWVGRWIGWLFALAMLGA
                     SAFGVYLGRFVRWNSWDALVSPFDIMVGAARVANPWNDVRPLAFSLTFFAFSLVCYLI
                     LYSFVHLHGWVQKASARPI"
     gene            complement(1651..2235)
                     /locus_tag="VFL84_03560"
     CDS             complement(1651..2235)
                     /locus_tag="VFL84_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET7607729.1"
                     /translation="MEKTICVYCASSQRCDAAYHAAAHRVGEVLAEHGFGIVYGGGAK
                     GSMGAVADGALARGGKVRGILPRFMQDLEWGHPKLTELRIVEDLRVRKHLMLSESDGL
                     VALPGGCGTLEELFEALTLKRLGLYVHPIVLLNTRNYFAPLIELLNHAVAERFMGERH
                     LEMWQVVAEPEQIPHALATAPPWSESAREFAAVR"
     gene            complement(2630..>2757)
                     /locus_tag="VFL84_03565"
     CDS             complement(2630..>2757)
                     /locus_tag="VFL84_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome BD ubiquinol oxidase subunit II"
                     /protein_id="HET7607730.1"
                     /translation="SSLMFVFVGVAIVLPFTIAYTIFVYRIFHGKATSLSYGEQD"
BASE COUNT          541 a          924 c          900 g          392 t
ORIGIN      
        1 ctcggcgccg cgcgcgtgga agcgaatcgc gacgaggctc gcggccgtcg cgcacgcgat
       61 cagcagcacg agcaacgaga ccatgaacag gccggacagc acgacccgca caccggcgac
      121 cagctcgtcg agcaggccga cgaacgcgta ctggaaatcg ttcggtcgcc cgtcggtcgc
      181 tacgccggcc attcgcgtcg cgagggcgtt ccgcgcggcc gcgcgcgagg ttccggccgc
      241 aagccggccg accgcgacga tctggcgctg gccgtagaga aagcgtggcg caccgaggtc
      301 cgacgccgag aagggaacat agacgagcgc ctcgcccgtg ctctccgtga acgacggcgg
      361 cagcacgccg acgacctcga cggacgcctg cgtgaacgtc agcgtgcgtc ccacggcgct
      421 cgggtcgccg ccggtgagct ccgtccaggc ctcgtggctc agcacggcca ctggccccgt
      481 ctcaccgaag tcggacgcta tgaacgtgcg gccgagggcg gccggcacgc cgaataccgt
      541 aaagaagtcg gcgctgacgc ggacgacacc gacgcggcgc gccaggccgg cgccgttgaa
      601 aaggtacggc ggctcgttga tgtagtacgc ggtgctctcg aatcccggca ggctcggcaa
      661 gccctccaag acttcggcgc gcgtcagtcc gccgcggccg gggccgcgcg acggagcccc
      721 cgtgtagatc cggaccaggc gatcggcgtc ggggtagggg agcgcgcgca gaaccacgcc
      781 gtgcagcacg ctgaacatgc ccgcgctgat gccgatgccg agcgcgacgg tgatgacgat
      841 cgcggccgcg ctcgcgggcc gccgcgtgag ccgtcgaagg gcgcgaatcc actcgtgcat
      901 cacgcgtgct ccctcggctg gtttccgagt atacgtctca aataggacgt gccgacgctt
      961 tctgcaccca gccgtgcagg tggacgaacg aatacaggat cagatagcag accagcgaaa
     1021 acgcgaagaa cgtcagggag aaggcgagtg gccgaacgtc gttccacgga ttcgcgacgc
     1081 gcgcagcgcc aaccatgatg tcgaacgggc tcacgagcgc gtcccacgag ttccagcgca
     1141 cgaaccgtcc gaggtaaacg ccaaacgcgc tcgcgccgag catcgcgagc gcaaaaagcc
     1201 agccaatcca ccggcccacc cacgcacgga cgacctcctg catgagatag agcgaaaggt
     1261 aaccgaggaa caaaccggtc tgcgcgaatg ccatcacgca gatgatgtcg taccaatacg
     1321 gcaccggccg tgcttcgcgc agatgcacga ggtccgtcac gatgtaaggc gcgttcggga
     1381 agaacaggaa ccaaatgact gccacggcga gaagcgccac ccagttgcgg ctctggcgcg
     1441 ccgcgagccc gtagaacgac aacgcggcac cgagcggcac ccaggcgagg aacagattcc
     1501 agaagaaaaa tagatagtcc gtggttcccg cgtagtaggc gcgcacgacg acgagcgcgc
     1561 agctcaacat cgacgcgagc acgagcgcga gaatcgcggc ccacgtttga cggaccggga
     1621 tcagccgttc gttcactgcg ccctgaatca tcagcgaacc gccgcgaact cgcgcgcgga
     1681 ttcggaccac ggcggcgccg tcgcgagcgc gtgagggatc tgctcgggct cggcgacgac
     1741 ttgccacatc tcgagatgcc gctcgcccat gaaccgctcc gccacggcat ggttcaggag
     1801 ctcgatcagc ggcgcgaaat agttgcgcgt gttcagcagc acgatcggat gcacgtacag
     1861 tccgaggcgc ttcagcgtca gcgcctcgaa caactcctcg agtgtgccgc agccgcccgg
     1921 caaggcgacg agcccgtcgc tctcggaaag catcaggtgc ttgcggacgc gcaagtcctc
     1981 gacgatgcgc agctccgtga gcttcgggtg gccccattcg agatcctgca tgaagcgcgg
     2041 caggatcccg cgcaccttgc cgccccgcgc aagggcgccg tccgcgacgg cgcccatcga
     2101 gcccttcgcg ccgccgccgt agacgatgcc gaagccgtgc tcggccagca cctcgccgac
     2161 gcggtgcgcg gcggcgtgat aggccgcgtc gcagcgttgg ctcgaggcgc agtagacgca
     2221 gatcgtcttt tccatgggca ctatgctatc cgaggcgcag cgcatgcgat cgcgacggcg
     2281 acgcgctgag gtgatctcat atcaactctt cggcggcttc gacgagcgtt tgcgagcggg
     2341 gccgttccag cgtccgaagt atctcctccg gctgaacccg cggcactcac cgcaccttcg
     2401 gagcgggtat cgcgcgatgc gcgcgctttt cgaaatcgag catgtcgacc aaccagcgca
     2461 atcgttcgcg aggtgacatc gcgagcattt gaggaatttg atcctctcct gcgacttctt
     2521 ctcgccccat gagctccagc cgggggtcgt ctggcgacgt gacaggcgtt ccgtcggcac
     2581 gcatgaggat attgagcaat cccaccttcg gtctgtcaaa ttggagcgct cagtcctgct
     2641 cgccgtagct caatgacgtc gctttgccgt ggaagatgcg gtagacgaag atcgtgtacg
     2701 cgatcgtgaa cggcaacacg atcgcgacac cgacgaacac gaacatcaat gagctcg
//
LOCUS       DASSNN010000197         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1425786, whole genome shotgun sequence.
ACCESSION   DASSNN010000197 DASSNN010000000
VERSION     DASSNN010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425786"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFL84_03570"
     CDS             <1..199
                     /locus_tag="VFL84_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607731.1"
                     /translation="VGVTGYIVYRSTSKNMSGETEVGRPTTTTFTDTSATRRTWYYAV
                     RAYDAAGNVSSRSNSQRVAVP"
     gene            244..792
                     /locus_tag="VFL84_03575"
     CDS             244..792
                     /locus_tag="VFL84_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET7607732.1"
                     /translation="MAAALAHAAVPKLDRVRVVETPRAIADAELTDEQGRTVHLRDLR
                     GKVVFVLFGFTNCEDVCPLTMERLGQLRDSGGLNAADVAYVMISVDGDRDTPAVMKQF
                     LARYPEELIGLTAPPNRVTPIAEQFSAAFFKGAHDAHGAYAVAHSPQIFVLDAAGNLR
                     AELFGASIESMTAVALALLAER"
     gene            complement(797..>2605)
                     /locus_tag="VFL84_03580"
     CDS             complement(797..>2605)
                     /locus_tag="VFL84_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET7607733.1"
                     /translation="LYAANTLGAAAGAAVGGWLLLGNLGFDGTVRLASSLNLGAAVIV
                     LVMWRVAAREATPAATAPGVPRAPAVANERVWPWLALYAATGAAALALELVYFRLVDG
                     IMRSNSYTFAHVLTLYLVLFGAGAAAAGERARRSARPDAAFLWIQFWIGVTSLVGVIA
                     LLNAPPLFGLRQSLEQYFRGEGFIYGFESVTDLRSGMAAVFAHLLAPALVMGAPVFLM
                     GYAYPFIAALVLRRESTLGRHTGALMACNILGNVAGTLIAGFVLLDVLGTAGTLALLA
                     ALLMALGVTAALRAPHARVQRAAAALGIAALLLVLFPTNERLWGFLHGAPPERFALVE
                     ERSCVNALVENGGVEMLHLNAAAQNGYPYDDFHVLIGLFPALLHPHPERALAVGLGIG
                     ATPYGMLLDTRLRNVETVEICAGLGDLLAQLGDLGAHESRQLLEDPRLDLRAGDGRKR
                     LLTAERRYDIITVDALRPQSGYSGNVYSVEFYELVASRLAPNGLFAQWVPTQRVLASV
                     MQVFPYVATTVGPGPATFLVASNEPLPDDLDQAVRRFHARPHGQLSAAQRASLGRFLE
                     HAKLTHVRRGEARTPVPETQLNRDLHPRDEYFLNDG"
BASE COUNT          437 a          888 c          920 g          360 t
ORIGIN      
        1 cgtcggtgtg acgggctaca tcgtctatcg atcgacgtcg aagaacatgt cgggggagac
       61 ggaagtgggc cggccgacga cgacgacgtt caccgacacg agcgcgacac gccgcacctg
      121 gtattacgcg gtcagagcgt acgatgcggc cggcaacgtc agtagtcgtt ccaactcgca
      181 acgggtggcg gtgccatgat gaggagctcg gtcgtccgtt ttgcagtggc aggagccgcg
      241 gccatggccg ccgccctggc ccacgccgcg gtcccgaagt tggatcgcgt gcgagtcgtc
      301 gagacgccgc gagcgatcgc cgacgccgag ctcacggacg agcagggccg cactgttcat
      361 ctacgcgacc tgcgcggcaa agtcgtgttc gtgctgttcg gcttcacgaa ctgcgaagac
      421 gtgtgcccgc tcacgatgga gaggctcggg cagctgcgcg acagcggcgg cttgaatgca
      481 gccgacgtcg cctacgtcat gatcagcgtc gacggcgacc gcgatacgcc cgcagtgatg
      541 aagcagttcc tggcgcgcta ccccgaggag ctcatcgggc tcacggcgcc gccgaatcgc
      601 gtgacgccga tcgccgagca gttctcggcc gcgttcttca agggcgctca tgacgctcac
      661 ggcgcgtacg ccgtggccca ctcgccgcag atcttcgtcc tcgacgccgc gggcaatctg
      721 cgcgccgagc tgtttggcgc gtcgatcgag tcgatgaccg ccgtcgcgct ggcgctgctc
      781 gccgaacgct agcctctcag ccgtcgttca agaaatactc gtcgcgcggg tgcaagtcgc
      841 gattgagctg cgtttccggc actggcgtgc gcgcctcgcc gcggcgcacg tgcgtgagct
      901 tcgcatgctc gaggaaccgc ccgagcgacg cgcgttgcgc ggcggagagc tggccgtgcg
      961 gccgcgcgtg aaagcgccgc acggcttggt cgagatcgtc cggcagcggc tcgttgctcg
     1021 cgacgaggaa cgtcgcgggt ccggggccga ccgtcgtcgc gacgtacgga aacacttgca
     1081 tcacgctcgc gagcacgcgc tgcgtgggca cccactgggc gaacaatccg ttcggcgcca
     1141 ggcgcgaggc tacgagctcg tagaactcga ccgagtacac gttgccgctg tagccgctct
     1201 gcggccgcaa cgcgtcgacc gtgatgatgt cgtagcgccg ctcggccgtg agcaagcgct
     1261 tgcggccgtc gccggcgcgc aagtcgagcc gcgggtcctc gagcaattgc cggctttcgt
     1321 gcgctccgag gtcgccgagc tgtgcgagca agtcgcctaa gcccgcgcaa atctcgacgg
     1381 tttcgacgtt gcgcaagcgc gtatcgagca gcattccgta cggcgtcgcg ccgatgccga
     1441 gcccgacggc gagcgcgcgc tcgggatgag gatgcagcag cgcggggaac aggccgatca
     1501 ggacgtggaa gtcgtcgtac ggataaccgt tctgcgcggc cgcgttcaag tgcagcatct
     1561 cgacgccgcc gttctcgacc agcgcgttga cgcagctgcg ctcttcgacg agcgcgaagc
     1621 gttccggcgg cgcgccgtgc aggaagcccc agagccgctc gttggtcgga aacagcacga
     1681 gcaacagggc cgcgatgccg agcgccgctg ccgcgcgctg cacgcgcgca tgcggcgccc
     1741 gcaacgcggc ggtcacgccg agcgccatga gcagcgccgc gagcagcgcg agcgttcccg
     1801 cggtgccgag cacgtcgagc agcacgaagc cggcgatcaa ggtccccgcg acgttgccga
     1861 ggatgttgca cgccatcagc gcgccggtgt ggcggccgag cgtgctctcg cggcgcagca
     1921 cgagcgccgc gatgaacgga tacgcgtagc ccatcaagaa caccggcgcg cccatgacga
     1981 gtgccggcgc gagcaagtgt gcgaacaccg cggccattcc ggaccgcagg tccgtgacgc
     2041 tctcgaagcc gtaaatgaaa ccctcgccgc ggaagtactg ctcgaggctc tgccggaggc
     2101 caaacagcgg cggcgcattc aagagtgcga tgacgccgac gaggctcgtg acgccgatcc
     2161 agaactggat ccacaggaac gcggcatcgg gccgcgcgga acggcgcgcg cgctcgccgg
     2221 ccgcggccgc gccggcgccg aacagcacga gataaagagt cagcacgtgc gcgaacgtgt
     2281 acgagttcga gcgcatgatg ccgtcgacga gcctaaagta gacgagctcg agcgcgagcg
     2341 cggctgcgcc ggtggcggca tagagcgcga gccacggcca cacgcgctcg ttggcgacgg
     2401 ccggcgcgcg cgggacgccg ggcgccgtcg ccgcaggcgt cgcttcgcgc gccgcgacgc
     2461 gccacatgac gagcacgatg acggccgcgc cgaggttcag gctgctcgcg agccggacgg
     2521 tgccgtcgaa cccgagattg ccgagcaaga gccaaccgcc caccgccgcg ccggccgccg
     2581 ccccgagcgt gttcgccgcg tacag
//
LOCUS       DASSNN010000198         4841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_169230, whole genome shotgun sequence.
ACCESSION   DASSNN010000198 DASSNN010000000
VERSION     DASSNN010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169230"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VFL84_03585"
     CDS             <1..689
                     /locus_tag="VFL84_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7607734.1"
                     /translation="RRTDNCKDMDHHAARVLSVGVSVAQRPDHPHNLLLAALPAADRR
                     RLLAQREPVELVLADTLIEPGDPVRHAFFPIDSLISLIAPISPRAQLEVGLVGNEGML
                     GIPLVLGVGVSDLRGLVQGAGLAWRVDAVTLRRELSLSPALRRRLNRYVFVSLSQLAR
                     TAACTRFHFVEARLARWLLMTRDRAHSNKFYITQEFLACMLGVRRAGVTRAAGALQRR
                     KLIRYRRGNL"
     gene            complement(825..1016)
                     /locus_tag="VFL84_03590"
     CDS             complement(825..1016)
                     /locus_tag="VFL84_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607735.1"
                     /translation="MKTLATLFVALLLAACENEGPLERAGEAADEAAEDVGDTAESAV
                     DAIDHAIDDVEDEVEGAAE"
     gene            complement(1123..2448)
                     /gene="nhaA"
                     /locus_tag="VFL84_03595"
     CDS             complement(1123..2448)
                     /gene="nhaA"
                     /locus_tag="VFL84_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010442272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET7607736.1"
                     /translation="MNESLGHLPNEPIDRFTKPFLRFLRIEATAGAALLLAIMLALVI
                     SNSPWANSFHAFWLTPVGVRVGAADFSHSLKHWINDGLMTLFFFLVALELKREIVLGE
                     LRNPRVAALSIAGALGGMLVPAVIYVFLARGSAGMAGWGTVMATDTAFVIGCLAILGS
                     RVPLSLRLFLLSLAIFDDIGAIVVVAIGYGEALNGWAVLLAGVGLTVVVGVGRIGVRN
                     MALYSLLGGLIWLAFDASGIHPTLAGVALGLLTPARVWVTGHRLRAILAKALVPPARE
                     QRSATAAEQENLRRARVATREAQSPVEQLERSLHPWVAFAILPVFALANGGVEISPEN
                     FNVSVAAAVFAGLVIGKPLGVVVVSFLAVRLGVAAKPAGLHWALIAAGGALTGIGFTM
                     AVFIAELAFDDAMLNSAKIGILAASVVAAAIGLLALAWLTSRSAMRAAP"
     gene            2587..2838
                     /locus_tag="VFL84_03600"
     CDS             2587..2838
                     /locus_tag="VFL84_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607737.1"
                     /translation="MSNDEATLQKLRSRYNAAFSTYQRRARVLFEATVSGNAPPEHLV
                     LQEDEALRELTQADRNLAEFIAAPRALGTKSRRAFRLDS"
     gene            2943..>4841
                     /locus_tag="VFL84_03605"
     CDS             2943..>4841
                     /locus_tag="VFL84_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7607738.1"
                     /translation="MGTYGPRRCGIATFTADLAAAVAGDARRVSPTVLAVTEPGGAYE
                     YPAEVKFEIRQEVKGDYARAAEFVNFNRVRLVCVQHEYGIFGGDDGGYILDFVHGLRI
                     PVIVTLHTVLKHPSDNQAAIVRKLAAASQLIIVMSEVAKGLLASSYGLDCANVRIVPH
                     GIPVMDLDPDQDALKAKFAVASRRVLLTFGLLSPNKGIETVIRALPTVVDSFPDLVYF
                     VVGATHPAILRREGEAYRTMLAREAEKLGVREHVVLRGQFVSAEELREYLQAADIFIS
                     PYLNEAQVTSGALSYAMGAGAAVVSTPYWHAQELLSNKRGRLFPFKDHAALSVALLEL
                     LRSPEELRRVRTAGFEFARLMAWPRIGDVYVEVIEQTLERCMQQPQLPARSPALAITL
                     PELRLDHLRRLTDDTGVIQHATYSVPARRTGYCVDDNARALIVALYADRLQSSAETRG
                     LVTTYLGYLHCSQEQDGSFRNFMSYERALESASPSDDCTGRALWALGLAVEIAQDDGC
                     RLLAREMFMRTLPHGRALGPRGTAQCILGLARLLAIDASSELRGVLDSLIAKLRERYR
                     ANATAGWRWFEPTLTYDNALLPLALFVAYGVTADRATLRDARESLEFIEEICFVGTRL
                     QLVGNTGWH"
BASE COUNT          880 a         1520 c         1551 g          890 t
ORIGIN      
        1 cgcgccgcac cgacaactgc aaggatatgg atcatcatgc tgctcgcgtt ctttccgtcg
       61 gagtttccgt ggcacaaagg cctgatcacc cgcacaacct tttgttggcg gctttgcccg
      121 cagccgaccg tcgccggttg ctcgcacagc gggagcccgt cgaactggtc ctggccgata
      181 cgctgatcga gcccggagac cctgtccgac atgcattctt cccgatcgac agcctcatct
      241 ctctaatcgc accgatcagc ccgcgtgccc agctcgaagt ggggctcgtg ggcaacgagg
      301 gaatgcttgg aatccccttg gtgctcggtg taggggtttc ggacctgcga gggttggtgc
      361 aaggtgcagg cctggcatgg cgagtggacg ctgtgactct tcgccgcgag ctgtcgctca
      421 gccccgcgct gcggcgccgt ttgaaccgat atgtcttcgt ctcattgagt cagctggcgc
      481 gcaccgcggc gtgcacgcgc tttcatttcg tagaagcgcg tctcgcgcgt tggttactga
      541 tgacgcggga tcgcgcgcat tcgaacaagt tttacatcac tcaagagttt ctcgcatgca
      601 tgctgggcgt gcggcgcgcc ggcgtgacac gagccgctgg cgcgttgcag cgccgtaagc
      661 tcattcgcta ccgtcgtggc aatctctaaa ttcgcagtgt gcgcggactc gaagcggcgg
      721 cttgtagttg ctacgcgagt ggcaaggaaa cctacgagcg agtgttctcg tagccggcgc
      781 gttgcatgct gtcggcggcc tcggggtgcg aagtccgaac cggcctattc agccgcgcct
      841 tcgacctcgt cttccacatc gtcgatagcg tgatcgattg cgtcgaccgc gctttccgcg
      901 gtgtcgccga cgtcttcggc agcctcgtct gccgcttctc cggcgcgctc gagcggcccc
      961 tcgttctcgc aagccgcgag cagcagcgcc acgaagagtg ttgcaagcgt cttcatgctg
     1021 aatctcctac cgattgccat cgagggccag ctcgtaatgc cggagtgacg cgaaattact
     1081 cgaaggtgtt acggcggtcg gtgcgctggc gcactttcga tttcaaggag cagcacgcat
     1141 ggcgctgcgc gacgtgagcc atgctaacgc gagcagaccg atcgcagccg cgacgaccga
     1201 cgcggccaag atgccaatct tggccgaatt caacatagcg tcgtcgaatg caagctcggc
     1261 tatgaagact gccatcgtga atccaatccc cgtaagcgcg cctccggctg cgatcaatgc
     1321 ccagtggaga cctgcaggct tggcggccac gccgagccgc accgcgagaa agctgacgac
     1381 cacgacgccg agtggcttgc caatcacgag gccggcgaaa acggcggcgg ctacggagac
     1441 gttgaaattc tccggcgaga tttcgactcc gccgtttgcc aaggcaaaga caggcaggat
     1501 cgcaaaggcc acccaagggt ggagcgacct ttcgagctgt tcgacgggag actgcgcctc
     1561 ccgcgtggcg acgcgcgcgc ggcggagatt ctcttgctcg gctgccgtgg cactgcgctg
     1621 ttcgcgtgcc ggcggaacta aagctttagc gagaatcgcg cgcagccggt gtccggtcac
     1681 ccacacgcga gccggtgtca atagccccag agcaacgccg gcgagtgtcg gatgaatccc
     1741 ggacgcatcg aatgcgagcc agatcaaacc tccgagcagg gaatagagcg ccatgttacg
     1801 gacgccgatg cgcccgacgc cgacgacgac ggtgagcccg acacctgcga gtagcaccgc
     1861 ccaaccgttc aatgcttcgc catagccgat cgcgaccacc acgattgcac cgatatcgtc
     1921 gaagatggcc agcgacaaca ggaatagacg aaggctcagc gggacccgcg agccgaggat
     1981 agccaggcac ccgatcacga acgccgtgtc ggtcgccatc accgtgcccc agcccgccat
     2041 gccggcactg cccctcgcca agaacacgta gatgacggcg ggcacgagca tcccgccgag
     2101 cgcgccggcg atcgagagcg cggccacacg cggattgcgg agctcgccaa gtacgatctc
     2161 ccgtttgagc tcgagcgcga cgagaaagaa gaacaaagtc atcaagccgt cgttgatcca
     2221 atgcttcagg gaatgagaaa aatcggcggc gccgactcgc acgcccaccg gtgtcagcca
     2281 aaatgcgtga aaactgttgg cccaaggaga gttcgatatc acgagcgcga gcatgatcgc
     2341 gagcaatagc gccgcgccgg ccgtcgcttc gatacggagg aatcgcagga acggcttcgt
     2401 gaagcggtct attggctcgt tgggaagatg tccgaggctt tcgttcattt atggccgtcg
     2461 cttgcggatt cctgacaaac tcgcgtgcgt tgtcggcgcc acgcggtgcg caagcctcga
     2521 tatgcgacct cgcgcgctat gtgctgcagc gaacggatcg aagctgcgat agtgggacaa
     2581 tgcacgatgt cgaacgacga agcgactctg cagaagctgc ggtcacggta caacgccgct
     2641 ttctccacat atcagcgccg tgcgcgcgtg ttgttcgagg cgaccgtgtc tgggaacgcc
     2701 ccgcccgagc acctcgtgtt gcaggaggac gaagcgctgc gcgagctgac gcaggcggac
     2761 cggaatttgg ctgaattcat cgcagccccg agggcgctcg gaacgaaatc gcgccgtgca
     2821 ttccgtctcg attcatgaag ggggcaagct tgtctgaaac ggcggttacc ttcggtgcgc
     2881 taccgcaccc actccgcacg ggcccgtttc gcgaagccga tgagggaatc gccgtcgcgt
     2941 tcgtgggtac gtacggtccg cgccggtgcg gcatcgcgac gttcacggcg gatctcgcgg
     3001 ccgccgttgc gggcgatgct cggcgcgtgt cgccgacggt gttggcggtc acggagccgg
     3061 gaggcgccta tgagtatccc gcggaggtca aattcgagat ccgtcaggaa gtgaagggcg
     3121 attacgcccg cgccgccgag ttcgtgaact tcaatcgagt gcggctcgtc tgtgttcagc
     3181 acgaatacgg aatattcggt ggcgacgacg gcggctacat tctggacttc gtgcacgggc
     3241 tgcgcattcc ggtcatcgtc acgctgcaca ccgtcctcaa gcatccttcc gacaaccaag
     3301 ccgctatcgt gcggaagctc gctgcggcga gccagctcat tatcgtcatg agcgaagtgg
     3361 cgaagggcct cctggcgagc tcgtacggcc tcgactgtgc gaacgtgcgg atcgtcccgc
     3421 acggcattcc ggtcatggat ctcgatcccg accaagacgc cctgaaggcg aagtttgccg
     3481 tcgcaagccg aagagttctg ctcaccttcg gcttgctgag cccaaacaag ggcatcgaga
     3541 cggtcattcg tgctctgccc acggtcgtcg acagcttccc ggacctcgtc tacttcgtgg
     3601 tgggcgcgac gcacccggcg atcctccgtc gagagggcga ggcgtaccgg acgatgctgg
     3661 cgcgcgaggc cgagaagctc ggcgtgcgcg agcacgtcgt gcttcgcgga cagttcgtgt
     3721 cggccgaaga gctgcgtgag tatctgcagg cggcggacat cttcatcagt ccgtatctga
     3781 atgaagccca agtcacgagc ggcgcgctgt cctatgcgat gggagccggc gctgccgtcg
     3841 tctctacgcc gtactggcat gcgcaggagc ttctttcgaa caagcggggg cggctgtttc
     3901 cgttcaaaga ccatgctgcg ctgagcgtcg cgttgctcga gctgctccgt tcgccggagg
     3961 agctccgacg agtgcgcacg gccggcttcg agttcgcgcg cttgatggcg tggccgagaa
     4021 tcggcgacgt ctacgtcgag gtcatcgagc agaccctcga gcggtgcatg cagcagcccc
     4081 agcttccagc gcgtagcccc gcgctcgcta tcaccttgcc ggagcttcgt ctggatcacc
     4141 tgcgtcgctt gacggacgac accggggtaa tccagcacgc gacttacagc gtaccggcgc
     4201 ggcggacggg gtactgcgtc gacgacaacg ctcgcgcgct catcgtcgcc ctgtatgcgg
     4261 atcgtctaca gagctcggcg gagacgcgtg ggctcgtgac tacgtacctg ggctacctcc
     4321 attgttccca agaacaggac gggagctttc gcaacttcat gagctacgaa cgagctctcg
     4381 agagcgcctc gccgtcggac gattgcaccg gacgcgcgct ctgggcgttg ggcctggccg
     4441 tcgagatcgc acaggacgac ggttgccgcc ttcttgcgcg agagatgttc atgcgtacat
     4501 tgccgcacgg tcgcgcgcta gggccgcgcg gtaccgcgca gtgcattctc gggctggcaa
     4561 ggttgctcgc gatcgacgcc tccagcgagc tgcgcggcgt tctcgattcg ctgatcgcga
     4621 agctacgaga gcgctatcgc gcgaacgcga ccgccggttg gcgctggttc gagcccacgc
     4681 tcacttacga caatgcactt ctgccgctcg cgctgttcgt ggcttacggc gtgacggcgg
     4741 atcgggcaac gctccgcgac gctcgcgagt ctctcgaatt catagaggag atctgcttcg
     4801 tgggcactcg gctgcagttg gtgggcaata cgggttggca c
//
LOCUS       DASSNN010000199         3643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1787178, whole genome shotgun sequence.
ACCESSION   DASSNN010000199 DASSNN010000000
VERSION     DASSNN010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787178"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /gene="ettA"
                     /locus_tag="VFL84_03610"
     CDS             <1..1469
                     /gene="ettA"
                     /locus_tag="VFL84_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HET7607739.1"
                     /translation="IKIGFLPQEPKLDPAKNVRAVVEEGVADQMQLVKRFNEISDKFA
                     EPLGDDEMNKLLEEQGKLQDAIDAIGGWELERKLDVAADALRLPPWDASVQSLSGGER
                     RRVALCRLLLSNPDMLLLDEPTNHLDAESVAWLERFLKEFPGTVIAVTHDRYFLDNVA
                     GWILELDRGHGIPWQGNYSSWLEQKEQRLAIEEKQEAARQRTIKTELEWVRTNPGARR
                     AKAKARLARFEELTSKEFQARNETNEIYIPPGPRLGDLVIEADKIGKAFGDRVLFENL
                     SFSLPPGGIVGVIGPNGAGKTTLFRIMAGQEKPDSGSVRIGNSVQLAYVDQSRDSLNN
                     DKTVWDEISHGQDIMRVGTYETPSRAYVGRFNFRGTDQQKKVGLLSGGERNRVHLAKL
                     LASGGNLLLLDEPTNDLDVETLRALEEALLNFPGSAVVISHDRWFLDRIATHILAFEG
                     NSEVVFFQGNYADYAEDLKRRKGQDADQPHRIRYKKLG"
     gene            1515..2219
                     /locus_tag="VFL84_03615"
     CDS             1515..2219
                     /locus_tag="VFL84_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607740.1"
                     /translation="MAIYVNKNNGFELTEPITRMCPHCGTHAQLLPVANPSYEVLEQT
                     RPRHAGLLFRCAACNEPRFLRASVRAIAPERVELAPNLVEVERVRERFQYGYLPDTIE
                     QLLRETLDCYSLGAHNAFATRCRRTTGAALRALGPTAKTRWQDTLLEMLRLCEVDAVT
                     TSTVDAVLFADGGDPPAVTPDEAAVLVEALKDLFYQSYVRSEKLRAAMKMRRFFAEES
                     LGTVTPIDREGWRELA"
     gene            complement(2229..3167)
                     /locus_tag="VFL84_03620"
     CDS             complement(2229..3167)
                     /locus_tag="VFL84_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET7607741.1"
                     /translation="MLVALPARAEWVGDAIDLMGTRVSVELWADDAARGRQLVAEVMR
                     EYHRVDDAMSTYKPDSEISRVNAHAADAPMVIGDELFKLVERSLDLSRASGGAFDITY
                     DSVGYLYDFRALKRPTDREIAERLPAVDYRHVVLDAERQTIFFTTHGVRINLGGIAKG
                     YVVERAAAMLRARGVEHALLNAGGDTRVIGDRRGQPWIVGIRHPRVADEVVTRLPLVD
                     EAISTAGDYERFFEENGRRYHHIINPATGRPTEGILTVTVIGPDGTLTDGLDTAIFVL
                     GVEKGLELIERYPDYETIIVDSTGKVSYSKGLVEPN"
     gene            3259..>3643
                     /locus_tag="VFL84_03625"
     CDS             3259..>3643
                     /locus_tag="VFL84_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET7607742.1"
                     /translation="MGTIITLVILAALVLYAISIYNNLVNSRNRVGNAFSQIDVQLTR
                     RYDLIPNLVEAVKGYMGHERGTLEAVINARNAAQAGLKKAAADPSDPDAIKALAAAET
                     TLGGTLGRLFALAEAYPDLKANQNMQ"
BASE COUNT          643 a         1243 c         1205 g          552 t
ORIGIN      
        1 gcatcaaaat cggcttcctg ccgcaggagc cgaagctcga tcccgccaag aacgtgcgcg
       61 ccgtcgtcga ggaaggcgtc gccgatcaaa tgcagctcgt caagcgcttc aacgagatca
      121 gcgacaagtt cgcggagccg ctcggcgacg acgagatgaa caagctactc gaggagcaag
      181 gcaagcttca ggacgcgatc gacgcgatcg gcggctggga gctcgagcgc aagctcgacg
      241 tcgcggccga tgcgctgcgc ttgccgccgt gggacgcgag cgtgcagtcg ctgtcgggcg
      301 gcgagcgccg ccgcgtcgcg ctgtgccgcc tgctgctgtc gaatcccgac atgctgctgc
      361 tcgacgagcc gacgaaccac ttggacgccg agtcggtcgc gtggctcgag cgcttcttga
      421 aggagttccc gggcaccgtc atcgcggtca cgcacgatcg ctacttcctc gacaacgtcg
      481 ccggctggat cctcgagctc gaccgcggcc acggcattcc gtggcagggc aactacagct
      541 cgtggctcga gcagaaggag cagcggctcg cgatcgagga aaagcaggaa gcggcgcggc
      601 agcgcacgat caagaccgag ctcgagtggg tccgcacgaa ccccggcgct cgccgcgcga
      661 aggccaaggc gcgcctcgcg cgcttcgagg agctgacctc gaaggagttt caggcacgca
      721 acgagacgaa cgagatctac attccgccgg gcccgcgcct cggcgatctc gtcatcgagg
      781 ccgacaagat cggcaaggcg ttcggcgatc gcgtgctgtt cgagaacctg tcgttcagcc
      841 tgccgccggg cggcatcgtc ggcgtcatcg gcccgaacgg cgccggcaag acgacgctgt
      901 tccgcatcat ggcgggccag gagaaaccgg acagcggcag cgttcgcatc ggcaacagcg
      961 tgcagctcgc ctacgtcgat cagagccgcg actcgctgaa caacgacaag acggtctggg
     1021 acgagatcag tcacggccag gacatcatgc gcgtcggcac ctacgagacg ccgtcgcgcg
     1081 cctacgtcgg ccgcttcaac ttccgcggca cggaccagca gaagaaggtc ggactgctgt
     1141 cgggcggcga acgcaaccgc gtgcacttag cgaagctgct cgcctcgggc ggcaacctgc
     1201 tgctgctcga cgagccgacg aacgacctcg acgtcgagac gctgcgcgcg ctcgaggagg
     1261 ccttgctcaa cttcccgggc agcgccgtcg tgatctcgca cgaccgctgg ttcctcgacc
     1321 gcatcgcgac ccacatcctc gcgttcgaag gcaatagcga ggtcgtgttc ttccagggca
     1381 attacgccga ttacgccgag gacttgaagc gccgcaaggg ccaggacgcc gatcagccgc
     1441 accggatccg ctacaagaag ctcggctagc gtgccggtcg cgcgcggggc tacactgaaa
     1501 gaaaaggccc cgtcgtggcg atttatgtaa acaagaacaa cggcttcgag ctcacggagc
     1561 cgatcacgcg catgtgcccg cattgcggca cgcacgcgca gctcttgccc gtcgcgaacc
     1621 cgtcctacga agtgctcgag caaacgcggc cacggcatgc gggcctgctg tttcgctgcg
     1681 cggcctgcaa cgagccgcga ttcctgcgcg cctccgtgcg tgcgatcgcg ccggagcgcg
     1741 tcgagctcgc gccgaatctc gtcgaggtcg agcgcgtccg cgagcgcttc cagtacggct
     1801 atctgcccga cacgatcgag caactgctgc gcgagacgct cgattgctac tcgctcggcg
     1861 cgcacaacgc gttcgcaacg aggtgccggc gcacgaccgg cgcggcattg cgcgcactgg
     1921 gcccgacggc caagacgcgc tggcaggaca cgctgctcga gatgctgcgc ctctgtgagg
     1981 tcgacgcggt gacgaccagc accgtcgacg cggtgctgtt cgcggacggc ggcgatcctc
     2041 ccgccgtcac gcccgacgag gcggccgtgc tcgtcgaggc gctcaaggac ctcttctacc
     2101 agagctacgt ccgcagcgag aagctgcgcg cggcgatgaa gatgcggcga ttcttcgccg
     2161 aggagagctt ggggacagtc acgccgatcg accgcgaggg ctggcgcgag ctcgcctgag
     2221 aaggcgcctc agttcggctc gacgagcccc ttcgagtacg agaccttgcc cgtcgagtcg
     2281 acgatgatcg tctcgtagtc cgggtaccgc tcgatcagct cgagcccttt ctccacgccg
     2341 agcacgaaga tggccgtgtc gagcccgtcg gtcagcgtgc cgtcggggcc gatgacggtg
     2401 accgtcagga tgccttccgt cggccggccg gtcgccgggt tgatgatgtg gtgatagcgg
     2461 cggccgttct cctcgaagaa ccgctcgtag tcgccggccg tcgagatggc ctcgtcgacg
     2521 agcggcagcc gcgtcacgac ctcgtcggcg acgcgcggat ggcgaatccc gacgatccag
     2581 ggctggccgc ggcggtcgcc gatgacgcgc gtgtcgccgc ccgcattgag cagcgcgtgc
     2641 tctacgccgc gcgcgcgcag catcgcggcc gcgcgctcga cgacgtagcc cttcgcgatg
     2701 ccgcccaagt tgatgcgcac gccgtgcgtc gtgaagaaaa tcgtctgccg ctcagcgtcg
     2761 agcacgacgt ggcggtagtc gaccgccggc aggcgctccg cgatctcgcg gtcggtcgga
     2821 cgcttaagcg cccggaagtc gtagagatag ccgacgctgt cgtacgtgat gtcgaacgcg
     2881 ccgccgctcg ctcgcgacag atcgagcgag cgctcgacga gcttgaacag ctcgtcgccg
     2941 atgaccatcg gcgcatcggc cgcgtgagcg ttgacgcgcg agatctcgct gtccggcttg
     3001 tacgtgctca tcgcgtcgtc gacgcggtgg tactcgcgca tgacctctgc gacgagctgc
     3061 cgaccgcgcg ccgcatcgtc ggcccagagc tcgaccgaga cgcgcgtgcc catcaagtca
     3121 atggcgtcgc cgacccattc ggcgcgcgcg ggaagcgcga cgagcatcac aaacgcggcg
     3181 gctgcgtgca gtgcccgata gcgcggcggc ggcgagcagc gtattctcgc ggcgtccgat
     3241 tcgggaagga gcttattcgt gggaacgatc attaccctcg tgatcctcgc ggcgctggtc
     3301 ctgtacgcga tctcgattta caacaacctc gtcaactcgc gcaaccgcgt gggtaacgct
     3361 ttctcgcaaa tcgacgtcca gctgacgcgc cgctacgact tgatccccaa tctcgtcgag
     3421 gccgtcaaag ggtacatggg ccacgagcgc ggcacgctcg aggccgtcat caacgcccgc
     3481 aacgccgcgc aagccggcct caagaaagcc gcggccgatc cgtccgatcc cgacgccatc
     3541 aaagccctgg ctgcggccga gacgaccctc ggcggcacgc tcggccgatt gtttgcgctc
     3601 gcggaggcct atcccgacct caaggcgaac cagaacatgc agc
//
LOCUS       DASSNN010000200         9419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_779361, whole genome shotgun sequence.
ACCESSION   DASSNN010000200 DASSNN010000000
VERSION     DASSNN010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779361"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VFL84_03630"
     CDS             <1..970
                     /locus_tag="VFL84_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET7607743.1"
                     /translation="RVGFDGSRPESLADGSRSWDGRRPPPGAIGGRDGALYTANSRTL
                     APSQADAVSRMWMRPLRAKRIDDLLAERRTFAERDFLSMQLDTRAEGYDRIRETILAV
                     VPSDDPEPKLMRARRLAEEWNGTADVDQAAFRLLQAYYRALLDRALTPLLAPAIEADP
                     SFVYRWPLADEVLRRLLDERPANLLTSEHADWPSFLRLSLLETIASIERGGALDAEWG
                     ETNVLDVAHPFAASFGPLGRFLALPRAPLPGSMVSLRVAAPGYGAVLRMSVSPSAPED
                     GVLELAGGQSGHFLSPTFRDLQRDWLEGKPTPFLAGEPTAQFKLTP"
     gene            complement(971..1966)
                     /locus_tag="VFL84_03635"
     CDS             complement(971..1966)
                     /locus_tag="VFL84_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607744.1"
                     /translation="MRQTTLLAAAAVALLAACTPEPESSTATSPPPAAAPGAAPASRP
                     LANAVVEVITAERGGFIPEGIEYDEDNGRFLTGSLAEGTIFVIERDGRVVPFIRDAEL
                     VSSVGIEADENRDRLLVANSNSAVFNDPNATGHAKLGVYHLTTGEKLAMVDLGATIGA
                     GARHFANDVTVDDDGNAYVTDTFANAIYKVTPDYKATVMRRFTDLPQGTSLNGIVHHP
                     GGYLLAVAEERIYKVPIANPAGTTQVNVSDPVEGQDGIVLTKDGRLVATSNSESEPRL
                     VAFMSNDNWASAERVAVATLNGQATTAAVVGDEIWAVHPHFGDAEPPTIERGVFR"
     gene            2078..2839
                     /locus_tag="VFL84_03640"
     CDS             2078..2839
                     /locus_tag="VFL84_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7607745.1"
                     /translation="MDQHTLLLAAVPAVGFAAGFINTLAGSGSLLTLPILILLGLPAN
                     VANGTNRVGVTMQNIVAVATFRRRGALPLTSSWPLIVPSIAGGIVGAMLAVDLDERLL
                     RQTIAVLMLVLIVLMLLKPERWIAAHAEPRKPRLLVEVPLFFAIGVYGGFLQVSVGLF
                     LIAGLVLAAGYELVGANAMKNLIVLLFTAAALVVFVVNDQVEWTLGLLLGAGQAVGAW
                     VAAHFAVKRGAEFVRFAVVIIAIGSAIALLTGLGR"
     gene            2852..3553
                     /locus_tag="VFL84_03645"
     CDS             2852..3553
                     /locus_tag="VFL84_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET7607746.1"
                     /translation="MTPQRHRERHRVRRVSWLRAAVLGANDGIVSTASLLVGVAAADA
                     SHGTLLTAGIAGAVAGAMSMAAGEYVSVSSQADSEKADLARERTELATDDAGERRELM
                     GIYMGRGLSQELAQRVADELMAHDALGAHARDELGITDLSVARPLQAACASALSFACG
                     ALLPLAVAAATSVSTALWAVPTTALFFLALLGALAARTGGAAIGTAAARVTFWGALAM
                     AATAGIGELFGSVVQ"
     gene            complement(3565..4995)
                     /locus_tag="VFL84_03650"
     CDS             complement(3565..4995)
                     /locus_tag="VFL84_03650"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7607747.1"
                     /translation="MKATRFAALLLLAPGLLAAQQGPDRSRPPGLGAPPRLMLPAIEK
                     HELKNGLDVWLVEAHEVPLVQVNLVVHAGSGDDPPSQYGLASLTAAMLDEGAGARSAL
                     EIADEIEFLGAELGATSSFDASAVRLNVPVRTMTSALSIMADVALRPTFPPDELERLR
                     EERLTALLQTRNDPAAVAAAALPRVVYGDSHRYGTIAIGTTATLSAFTPQDLAAFHAR
                     KYRPTNSTLIVVGDVTASSVLPLLENAFGGWTAERAAARTAVPAAPGPRTRQVTIVDM
                     PGAEQSAIRVGTVGVARSTPDYFALQVLNTILGGSFTSRLNQNLREEHGYAYGASSRF
                     DMRLAPGPFVVAANVQTDKTAESLTEIFNELEAIAEPIESDELAKAKNYLALSFPAAF
                     ETIEDLAARIEELAIYGLPDSYYADYTNAIGAVTAESVQRAAAAHIDPARLSVVVVGD
                     RSKIEAGIRALELGPVRVVGVEDVVP"
     gene            complement(4992..6347)
                     /locus_tag="VFL84_03655"
     CDS             complement(4992..6347)
                     /locus_tag="VFL84_03655"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7607748.1"
                     /translation="MRSSFVSLLALAAASLASPGGAQTAAPLEVPFRQFTLDNGLNVI
                     LHRDASVPIVAVNVWYHVGSANEKMGRTGFAHLFEHLMFEGSKNVPEGEFDKLLEAAG
                     GDNNGSTNADRTNYVIDVPANALELALFLESDRMGSLLDTMTPDVVDGQRDVVKNERR
                     QSYENRPYGMAELALDELTWPEGHPYHWPTIGYMEDLTAASYDDVVAFFSTYYTPNNA
                     SLAVAGDIDLDETQALVEKWFAGIPRRADVLPVAAPPAALDGVTRKTMKDRVGLQRLY
                     LAWITPRHFAPGDSTLDLVSAVLAGGKNSRLYKRLVYDLQIAQDVSAFQASAALGSQF
                     LIVATARPEHTAAELKTVIDEELTRLQREAPAAREVERAVNQIEASFYRGMERVGGFG
                     GKADQLNAYFAAGGGPDYFAEDLARYTSLSPADVRAAAAQWLPLDRRVELVVEPEEQS
                     P"
     gene            6439..7185
                     /locus_tag="VFL84_03660"
     CDS             6439..7185
                     /locus_tag="VFL84_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET7607749.1"
                     /translation="MTATARAFGVARSLAMYYGIPFRGRRLRRLYSQFVHAGALCFDV
                     GAHAGNRVRCWRSLGARVVAIEPQPDFVRVLRWLYGDDDGVEIVPHAVGRSAGSAELL
                     LSERTPTVTTLSQSFIDNVRQDPGFANVKWSRGQRVDVVTLQSLVERHGEPAFVKIDV
                     EGFEAEVLAGLATPVRALSFEYVPATRDVALECVERLAALAQYRYNWSVGETHRLASA
                     HWLDSAGLRTLLSGLPKRSPSGDVYAVRAD"
     gene            complement(7189..8805)
                     /locus_tag="VFL84_03665"
     CDS             complement(7189..8805)
                     /locus_tag="VFL84_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607750.1"
                     /translation="MSSKASLSTAQWIALALALLVLNAAMTFGNVWPTLRVVPRAELS
                     IEIAVLLLALVAWTSFMRPLGRASLAALTLLLLVFVLGRYAEVTAPALYGRPVNLYWD
                     SQHLPKVAAMLAEVGPWWLVGLLAVALLALFAALITALALCLGAVARALRVPAGRRAV
                     GALAGSIVALYALASWLDWPARHKFSLPVAATYLQQARFVLAAWKADPNRDLPGLSLP
                     PSNLGRLEGDDVLLLFLESYGAVTYDEPSIARIVSPARDELAHAAAETRRHVVSAYAS
                     SPTFGGGSWLAHASFMSGVEVRDTGDYMLLLTQRRETWPKLFEAAGYRAVAVMPGLRS
                     AWPEGAFYGFDTIYDALALDYRGPDFGWWRIPDQFSLARTAELEAHSDSHAPLLVFMP
                     TINTHIPFLPVPPYQADWNRVLKADPYTPDDVEASLAPVPDWEALGGPYADSFVYTFT
                     YLAGYVRDRAGADQTLVLLGDHQPAASVAGVGARWDVPVHVITSHADIAAALVAAGFV
                     DGVALAPQQRPIATLPELSALLLDTFAADR"
     gene            complement(8802..>9419)
                     /locus_tag="VFL84_03670"
     CDS             complement(8802..>9419)
                     /locus_tag="VFL84_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7607751.1"
                     /translation="GSGSVVVRAAVLAAAGGAIFLWLAMRRLHALKFGAANSVTLARA
                     AMTVLLAALVGSAPTPALGWVLVGIGTVAVALDGVDGALARSRAEAGAFGARFDMETD
                     ALLILVLAALVWQQGKAGAWILAAGLLRYLFVAGSAILPWLGAVLPPSRRRQAVCVVQ
                     IVSLLGALAPVVALPWSAALALAGLAALVWSFALDVLWLARQARA"
BASE COUNT         1416 a         3284 c         3312 g         1407 t
ORIGIN      
        1 ccgcgtcggc ttcgacggct cgcggcccga gtcgctcgcc gacggcagcc gctcgtggga
       61 cggccgccgg ccgccgccgg gcgcgatcgg cggccgagac ggcgcgctct acacggccaa
      121 cagccgcacg ctggcgccga gccaagccga cgccgtgagc cgcatgtgga tgcggccgct
      181 gcgcgcgaag cgcatcgacg acctgctcgc cgagcggcgc acgttcgccg agcgcgactt
      241 cctttcgatg cagctcgaca cgcgcgccga aggctacgac cggattcgcg agacgatcct
      301 cgcggtcgtt ccgagcgacg atcccgagcc caagctcatg cgtgcgcgcc gtctcgccga
      361 ggagtggaac gggactgccg acgtcgacca agccgcgttt cggctgctgc aggcgtacta
      421 ccgcgcgctg ctcgaccgcg ccctgacgcc gctgctcgcg cccgcgatcg aggcggatcc
      481 gagcttcgtc taccgctggc cgctcgcgga cgaagtgctg cggcggctgc tcgacgagcg
      541 gcccgcgaat ctcttgacga gcgagcacgc ggactggccg tcgttcttga ggctgtcgct
      601 gctcgagacg atcgcctcga tcgagcgcgg cggcgcgctc gacgccgagt ggggcgagac
      661 caacgtgctc gacgtcgcgc atccgttcgc ggcgagcttc ggccctctcg gccgctttct
      721 cgcgctgccg cgcgcgccgc tgccgggctc gatggtgtcg ctgcgtgtcg ccgcgccggg
      781 ctacggcgcc gtgctgcgca tgtccgtttc gccgagcgct cccgaagacg gggtgctcga
      841 gctcgccggc ggccagagcg gccatttctt gtcgccgact ttccgcgacc tgcagcggga
      901 ctggctcgaa gggaagccga cgccgttcct cgccggcgag ccgacggcgc aattcaagct
      961 gacgccgtag ctaccgaaac actccgcgtt cgatcgtcgg cggctccgcg tcgccgaaat
     1021 gcggatgcac ggcccaaatc tcgtcgccga cgaccgcggc ggtcgtcgct tggccgttca
     1081 aggtcgcgac ggcgacgcgc tctgccgacg cccagttgtc gttgctcatg aacgcgacga
     1141 gccgcggctc gctctcgctg ttgctcgtcg cgactagccg gccgtctttc gtcagcacga
     1201 tgccgtcctg gccttcgacc ggatcgctga cgttgacttg cgtcgtgccg gcggggttcg
     1261 cgatcggcac cttataaata cgctcttcgg cgacggcgag cagataaccg cccgggtgat
     1321 ggacgatgcc gttcagcgac gtgccctgcg gcaagtccgt gaaccgccgc atcacggtcg
     1381 ccttgtagtc cggggtcacc ttgtagatcg cgttcgcgaa cgtgtccgtc acgtacgcgt
     1441 tgccgtcgtc gtcgacggtg acgtcgttcg cgaagtgacg cgctccggcg ccgatcgtcg
     1501 cgccgagatc gaccatcgcg agtttctcgc ccgtcgtcag gtgatagacg ccgagcttgg
     1561 cgtgacccgt cgcgttcggg tcgttgaaga cggcgctgtt cgagttcgcg acgagcagcc
     1621 ggtcgcgatt ttcgtcggcc tcgatgccga ccgacgacac gagctcggcg tcgcgaatga
     1681 acggcacgac acgaccgtcg cgctcgatga cgaagatcgt gccttcggcg agcgagcccg
     1741 tcaggaaccg gccgttgtcc tcgtcgtact cgatgccttc ggggatgaag ccgccgcgct
     1801 cggccgtgat gacctcgacc acggcgttgg cgagcggacg cgatgccgga gcggcccccg
     1861 gagccgccgc cggagggggc gacgtggccg tcgaggattc gggctccggc gtgcaggccg
     1921 cgaggagcgc aacggccgcc gcagcgagca aagtcgtctg acgcatggca aggccctcca
     1981 atcgtgagcg actcccggca tcgtaacact cgggggtggc gtgccgggcc acgttgtggc
     2041 tagagtgcgg caccgtcgcc gcgagcatag cgcctgcatg gatcaacaca cgctgctgct
     2101 cgccgccgtg ccggccgtgg gcttcgccgc cggcttcatc aacacgctgg ccggcagcgg
     2161 ctcgctgttg actctgccga tcctgatctt gctcggcctg ccggccaacg tcgcgaacgg
     2221 cacgaatcgc gtcggcgtga cgatgcaaaa catcgtcgcg gtcgcgacgt ttcgccggcg
     2281 cggcgcgctg ccgctcacga gctcgtggcc gctcatcgtg ccgtccatcg cgggcggaat
     2341 cgtcggcgcg atgctggccg tcgatctcga cgagcggctg ctgcgccaga cgatcgcagt
     2401 gctgatgctc gtgctgatcg tcttgatgct gctcaagccc gagcgctgga tcgcggcgca
     2461 cgcggagcct cgtaagccgc gcctgctcgt cgaggtgccg ctgttcttcg cgatcggcgt
     2521 ctacgggggc ttcctccagg tcagcgtcgg gttgttcctg atcgcgggcc tcgtgctggc
     2581 cgcgggctac gagctcgtcg gcgccaacgc gatgaagaat ctcatcgtgc tgctgttcac
     2641 ggcggcagcc ctcgtcgtct tcgtcgtcaa cgatcaggtc gaatggacgc tcggcctgtt
     2701 gctcggggcc ggacaagccg tgggcgcctg ggtggccgcg catttcgcgg tcaagcgggg
     2761 cgcggagttc gtgcgcttcg cggtcgtcat catcgcgatc ggctccgcga tcgcgctgct
     2821 caccgggctc ggacggtaga ctagcgatcg catgactccc caaagacatc gagaacggca
     2881 tcgcgtgagg cgagtcagct ggctgcgggc ggcggtgctc ggggccaacg acggcatcgt
     2941 ctcgacggcg agcttgctcg tcggcgtcgc cgcggccgac gcgagccacg gcacgctgct
     3001 gacggccggc atcgcgggcg ccgtggccgg cgcgatgtcg atggccgcgg gcgagtacgt
     3061 ctcggtgagc tcgcaagccg acagcgagaa ggcggacctc gcgcgcgagc gcaccgagct
     3121 tgcgaccgac gacgcgggcg agcgccgcga gctgatggga atttacatgg gccgcggttt
     3181 gagccaggag ctcgcgcagc gcgtcgccga cgagctcatg gcgcacgacg cgctcggggc
     3241 gcatgcgcgc gacgagctcg gcatcaccga tctgtcggtc gcgcggccgc tgcaggcggc
     3301 gtgcgcgtcg gcattgagct tcgcctgcgg cgctctgctg ccgctcgcgg tcgcagcggc
     3361 cacgtcggtg tccaccgcgc tctgggccgt gccgacgacg gccctattct tcctcgcgtt
     3421 gctcggcgcg ctcgcggcgc gcacgggcgg cgccgcgata ggcaccgcgg cggcgcgcgt
     3481 cacgttctgg ggcgcgctcg cgatggccgc gacggccggc atcggcgagc tattcggcag
     3541 cgtcgtgcag tgagcgcggc ggcgtcaggg gacgacgtcc tcgacgccga ccacgcgcac
     3601 ggggccgagc tcgagcgcgc gaattccggc ttcgatcttc gagcgatcgc cgaccacgac
     3661 gacggacaac ctcgccgggt cgatgtgcgc cgccgcggcg cgctgcacgg attcggccgt
     3721 gaccgcgccg atcgcgttcg tgtaatcggc gtagtagctg tccggtagac cgtagatcgc
     3781 gagctcctcg atgcgagccg caaggtcttc gatcgtctcg aatgcggcgg ggaaactcag
     3841 ggccagatag ttcttggcct tcgcgagctc gtcgctttcg atcggctcgg cgatcgcttc
     3901 gagctcgttg aatatttccg tcaacgactc ggccgtcttg tcggtttgga cgttggccgc
     3961 aacgacgaac ggtccgggcg cgagccgcat gtcgaaccgc gagctcgcgc cgtacgcgta
     4021 gccgtgctcc tcgcgcaggt tctggttcag ccgcgacgtg aacgacccgc cgagaatggt
     4081 gttcagcact tgcagcgcga aatagtccgg agtcgagcgc gcgacgccga cagtgccgac
     4141 gcgaatcgcg gattgctcgg cgccgggcat gtcgacgatc gtgacctgcc gcgttcgtgg
     4201 ccccggcgcc gcgggcaccg cggttctcgc cgcggcgcgc tcggcggtcc aaccgccgaa
     4261 cgcgttctcg agcagcggca acacactcga agccgtgacg tcgccgacca cgatcagcgt
     4321 cgaattcgtc ggccggtact tccgcgcgtg aaacgccgcg aggtcttgcg gcgtgaatgc
     4381 cgagagcgtc gccgtcgtgc cgatcgcgat cgtcccgtag cggtgcgagt cgccgtagac
     4441 gacgcgcggc aacgcggccg ccgccaccgc ggccgggtcg ttgcgcgtct gcagcagcgc
     4501 cgtgagccgc tcctcgcgca agcgctcgag ctcgtcgggc ggaaacgtcg gccgaagcgc
     4561 cacgtcggcc atgatcgaca gcgcgctcgt catggtccgc accggcacgt tcaagcgcac
     4621 ggccgacgcg tcgaacgagc tcgttgcgcc tagctccgcg ccgaggaact cgatctcgtc
     4681 cgcgatctcg agcgcgctgc gcgcgccggc gccttcgtcg agcatcgccg cggtcaggct
     4741 cgcgagcccg tactgcgacg ggggatcgtc gccgctgccg gcatggacga cgagattgac
     4801 ttgcacgagc ggcacctcgt gcgcctcgac gagccacacg tcgagaccgt tcttgagctc
     4861 gtgcttctcg atcgccggca gcatcaaacg cggcggggca ccgagcccgg gcggccgcga
     4921 gcgatcgggc ccctgctgtg cggccagcaa gcccggagcc aagagcagga gggctgcgaa
     4981 gcgcgtggct ttcatgggct ctgctcctcc ggctcgacga cgagctcgac gcgccggtcg
     5041 agcggcagcc attgcgccgc ggccgcccgc acgtccgcgg gcgaaagcga cgtgtagcgc
     5101 gcgagatcct cggcgaaata gtcgggcccg ccgcccgccg cgaagtaagc gttcagctgg
     5161 tcggccttgc cgccgaaccc gccgacgcgc tccatgccgc gatagaacga tgcctcgatc
     5221 tgattcacgg cgcgctcgac ctcgcgcgcg gccggtgcct cgcgctgcaa gcgcgtgagc
     5281 tcctcgtcga tgaccgtttt gagctcggcc gccgtatgct cgggccgcgc cgtcgcgacg
     5341 atcaaaaact ggctgccgag cgccgccgag gcttgaaatg cggagacgtc ttgcgcgatc
     5401 tgcaagtcgt agacgagccg cttgtagagc cgcgagttct tgccgcccgc aagcacggcc
     5461 gaaacgaggt cgagcgtcga gtcgccgggc gcgaagtgcc gcggcgtgat ccaggcgaga
     5521 tagagccgct gcaagccgac gcgatccttc atcgtcttgc gtgtcacgcc gtcgagcgcg
     5581 gccggcggcg ccgctaccgg caacacgtcc gctcggcgcg gaatccctgc gaaccatttc
     5641 tcgacgagcg cttgcgtctc gtcgaggtcg atgtcgccgg cgacggcgag gctcgcgttg
     5701 ttcggcgtgt aataagtcga gaagaacgcg acgacgtcgt cgtagctcgc cgcggtcaga
     5761 tcttccatgt agccgatcgt cggccagtga tacggatggc cctccggcca cgtgagctcg
     5821 tcgagcgcga gctcggccat gccgtacggc cggttctcgt agctctggcg gcgctcgttc
     5881 ttgacgacgt cgcgctggcc gtcgacgacg tccggcgtca tcgtgtcgag cagggagccc
     5941 atgcggtcgg actcgaggaa cagcgcgagc tcgagcgcgt tggccggcac gtcgatgacg
     6001 tagttcgtgc ggtcggcgtt cgtcgagccg ttgttgtcgc cgccggccgc ttcgagcagc
     6061 ttgtcgaact ccccttcggg cacgttcttc gagccttcga acatcaggtg ctcgaacaag
     6121 tgcgcgaacc cggtccggcc catcttctcg ttggccgagc cgacgtggta ccagacgttc
     6181 acggcgacga tcggcacgct cgcatcgcga tgcaggatca cgttgagccc gttgtcgagc
     6241 gtgaattgcc ggaaaggcac ctcgagcggc gcggccgtct gcgccccgcc cggcgaggcg
     6301 aggctcgcgg ctgcgagcgc aagcaacgag acgaaggacg agcgcacggc ggtctcccgt
     6361 ggacggttca gtcgatcttg cagcgcgcgc ggccgcgcca caagcggcca taagcgtctc
     6421 cgctagaatc gactttgaat gacggctacc gccagagcgt tcggcgtcgc gcgctcgctc
     6481 gcgatgtatt acggcatccc cttccggggg cgccggctgc gccgcttgta ttcgcaattc
     6541 gtgcacgcgg gcgcgctctg cttcgacgtc ggcgcccacg ccggcaatcg cgtgcgctgc
     6601 tggcgcagtc tcggcgcgcg cgtcgtggcg atcgagccac agcccgattt cgtgcgagtt
     6661 ttgcgctggc tgtacggcga cgacgacggc gtcgagatcg tgccgcatgc ggtcggccgc
     6721 agcgcgggca gcgccgagct gttgctgagc gagcgcacgc cgaccgtcac gacgctgtcg
     6781 cagagcttca tcgacaacgt gcggcaagac cccggcttcg cgaacgtgaa gtggtcgcgc
     6841 ggccagcgcg tcgacgtcgt cacgctgcaa tcgctcgtcg agcggcacgg cgagcccgcg
     6901 ttcgtcaaga tcgacgtcga aggcttcgag gcggaggtgc tcgccgggct cgcgacgccc
     6961 gtgcgcgcgc tgtcgttcga gtacgttccg gcgacgcgcg acgtcgcgct cgagtgcgtc
     7021 gagcggctcg ccgcgctcgc gcagtaccgc tacaactggt cggtcggcga gacgcatcgg
     7081 ctcgcgagcg cgcactggct cgactcggcc ggattgcgaa cgctgctgag cgggctgccg
     7141 aagcgctcgc cgtcgggcga cgtctacgcc gtgcgcgcag actgaagctc agcgatccgc
     7201 cgcgaacgta tcgagcagca gggccgacag ctccggcagc gtggcgatcg gccgctgttg
     7261 cggcgcgagc gcgacgccgt cgacgaatcc cgccgcgacg agcgccgccg caatgtcggc
     7321 gtgcgacgtg atgacgtgga cgggcacgtc ccagcgcgcg ccgacgcccg cgacgctcgc
     7381 ggcgggctga tggtcgccga gcagcacgag cgtctggtcg gctcccgctc tgtcccgaac
     7441 gtagccggcg agatacgtga acgtgtagac gaacgagtcg gcgtacgggc cgccgagagc
     7501 ctcccagtcg ggcacggggg cgaggctcgc ctcgacgtcg tccggcgtat acgggtcggc
     7561 cttcaggacg cgattccagt cggcttgata cggcggcacc ggcagaaacg gaatgtgcgt
     7621 gttgatcgtc ggcatgaaca cgagcagcgg cgcgtgcgag tcgctgtgcg cctcgagctc
     7681 ggcggtgcgc gcgagcgaga actggtccgg aatccgccac cagccgaagt cgggcccgcg
     7741 gtaatcgagc gcgagcgcgt cgtagatcgt gtcgaagccg tagaacgccc cctcgggcca
     7801 ggcgctgcgc aggccgggca tgacggccac ggcgcggtag ccggccgcct cgaacagctt
     7861 cggccaggtc tcgcgccgct gcgtcagcaa cagcatgtaa tcgcccgtgt ctcgcacctc
     7921 gacgccgctc atgaagctcg cgtgcgcgag ccacgagccg ccgccgaacg tcggcgacga
     7981 cgcgtacgcg gacacgacgt gccgccgcgt ctcggcggcg gcgtgcgcga gctcgtcgcg
     8041 cgccggcgaa acgatgcgcg cgatgctcgg ctcgtcgtac gtcacggcac cgtaggactc
     8101 gaggaacaac agcagcacgt cgtcgccttc gagccttccc aaattcgacg gcggcagaga
     8161 caggcccgga agatcgcggt tcggatcggc cttccaggcc gcgagcacga accgagcttg
     8221 ctgcaggtac gtcgccgcga ccggcagcga gaatttgtgc cgggccggcc agtcgagcca
     8281 agacgcgagc gcgtagagcg cgacgatcga gcccgcgagc gcgcccacgg cgcggcggcc
     8341 cgccggcacg cgcagcgcgc gcgccacggc gcccaaacac aaggcgagag ccgtgatcaa
     8401 cgcggcgaac agcgcgagca acgcgacggc gagcaagccg acgagccacc acggccctac
     8461 ctcggcgagc atcgccgcga ctttcggcaa gtgctggctg tcccagtaca ggttcacggg
     8521 ccggccatac agcgccggcg ccgtgacctc ggcgtaacgg ccgagcacga agacgagcaa
     8581 gagcaacgtc aacgccgcga gcgacgcgcg gccgagcggc cgcatgaacg aagtccacgc
     8641 gacgagcgcg agcaacagca cggcgatctc gatcgacagc tcggcgcgcg gcacgacgcg
     8701 cagcgtcggc cacacgttgc cgaacgtcat agccgcgttc aggacgagca gcgcgagcgc
     8761 caacgcgatc cactgcgccg tcgagagcga tgccttcgag ctcacgcgcg cgcctgccgt
     8821 gcgagccaca gcacgtcgag cgcgaacgac cacacgagcg ccgcgagccc cgcaagcgcg
     8881 agcgcggcgc tccagggcaa cgcaacgacg ggtgcgagcg cgccgagcag cgacacgatc
     8941 tgcacgacgc aaacggcttg acggcgccgg ctcggcggca gcactgctcc aagccaggga
     9001 aggatcgcgc ttccggcgac gaacagataa cgcagaagcc ccgccgcgag gatccaagcg
     9061 ccggccttgc cctgctgcca gacgagcgcc gcgagcacga ggatcagcaa cgcgtcggtc
     9121 tccatgtcga agcgcgcgcc gaacgcgcct gcctcggcgc ggctgcgcgc gagcgcgcca
     9181 tcgacgccgt cgagcgcgac ggcgaccgtc ccgatcccca cgagcaccca gccgagcgcg
     9241 ggcgtcggcg ccgagccgac gagcgcggca agcagcaccg tcatcgcggc tcgcgcgagc
     9301 gtcaccgagt tcgcggcgcc gaacttgagc gcatgcagcc gccgcatcgc gagccaaagg
     9361 aaaatcgcgc cgccggcggc ggcgagcacg gccgctcgca cgacgacgga gccgctccc
//
LOCUS       DASSNN010000201         3933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1050072, whole genome shotgun sequence.
ACCESSION   DASSNN010000201 DASSNN010000000
VERSION     DASSNN010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050072"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..675)
                     /gene="azu"
                     /locus_tag="VFL84_03675"
     CDS             complement(229..675)
                     /gene="azu"
                     /locus_tag="VFL84_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008483242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HET7607752.1"
                     /translation="MRRTFWVVALGGLLLSGTAWSQECSLSIEGNDQIQFNKKELRVS
                     KSCKEVTVTLKHVGQLAANVMGHNWVLTATADYQAVAAAGQAAGPPNYVPAGDARVLA
                     HTNVVGGGQETSVKFDLSKLTPGGDYTYFCTFPGHFVLMNGKLIVE"
     gene            994..1431
                     /gene="dtd"
                     /locus_tag="VFL84_03680"
     CDS             994..1431
                     /gene="dtd"
                     /locus_tag="VFL84_03680"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET7607753.1"
                     /translation="MIGLIQRVSGAQVTVNAQKIAQIGRGLLVLVGVEREDGPEQAQK
                     LASRLVAYRVFPDDAGKMNLSVREVGGALLLVPQFTLAADTSAGLRPSFSRAAPPEVG
                     SRLFDALVAAVEATGVPHARGRFGADMQVTLTNDGPVTFWLQA"
     gene            1503..1739
                     /gene="tatA"
                     /locus_tag="VFL84_03685"
     CDS             1503..1739
                     /gene="tatA"
                     /locus_tag="VFL84_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007018413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HET7607754.1"
                     /translation="MGFGSIWHWLIVLAIVVLIFGTKKLKSAAGDIGGAVKDFKKAMS
                     DDDKPSEEAQKRLESDEQDAEFPEQKKKNKNRSA"
     gene            1754..2107
                     /gene="tatB"
                     /locus_tag="VFL84_03690"
     CDS             1754..2107
                     /gene="tatB"
                     /locus_tag="VFL84_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HET7607755.1"
                     /translation="MFDIGPGELLLVAIIGLLVLGPQRLPKVAAEIGKWDGRARRTAS
                     QLRRQLEREIELSELETPPPAPPPPPAATPSPAPAATNGSGQATTDAEAGEANSIGGH
                     AASSEPSSAAEKREG"
     gene            2107..2883
                     /gene="tatC"
                     /locus_tag="VFL84_03695"
     CDS             2107..2883
                     /gene="tatC"
                     /locus_tag="VFL84_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET7607756.1"
                     /translation="MSAKPDEPEEQLAEGTLMSHLLELRSRIMKALLAVIVVFLVLAY
                     WMNDVFELVSKPLRDALPEGSEMIVTDVAAPFLTPMKTAFFVALFIAMPVVLFQAWQF
                     VAPGLYKREKRLAIPLLLSSIALFYAGIAFAYFIVFKVMFGFFARVVPGGVQMSPDIS
                     AYLSFVLGVFLTFGLAFEVPIATVLLIWSGLVSVKTLKNIRPYVFLGAFVAGMLLAPD
                     VFSMTLMAIPMYALYEAGLLMARFLVPEKMAAETAPAQKS"
     gene            2880..>3933
                     /locus_tag="VFL84_03700"
     CDS             2880..>3933
                     /locus_tag="VFL84_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020682530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HET7607757.1"
                     /translation="MRRIDSKLPAVGTTIFTLMSQLAEQHGAVNLSQGFPDFRPPAGL
                     LELVERCLRERFHQYALMPGSMPLRTAIAQKLAAMYDVTVDPAAEITVTSGATEAIFD
                     AIQAVVRRDDEVIVFDPCYDSYEPSIMLAGGRTIHLPLTQPDFAIDWERLRAALSRRT
                     RLVIVNSPHNPSGALLGAADFDRLAEALRPYDCYLLSDEVYEHVVFDGARHATALAQR
                     ELKERCFAVFSFGKTYHATGWKLGYCVAPAELSAELRRVHQYVTFASTSPLQQALAEY
                     MTRHPEHHVGLPAFYQERRDYFTALLEPTKFRLRRAAGTYFQLADYGAISTLPDVEFA
                     RWLTIEHGVATIPISVF"
BASE COUNT          702 a         1265 c         1245 g          721 t
ORIGIN      
        1 acgtgacgtc gaccgtgaag tcgatcgtgc tccagttcga agccagcggt gtgaaatacc
       61 agccgagagg cccgctcgcg aaatgaaaga tcagcgaccc gacaacgagc aggacgatga
      121 cgactgcaag agccaccata cttgaaacac cccccaaaac attaggcgca cgatctgccg
      181 tcgcggtcct agtgtgtcga agaacggctc aacccgccgc cgcgggcact attcgacgat
      241 cagctttccg ttcatcaaca caaagtggcc agggaacgtg cagaaatacg tgtaatcgcc
      301 gccgggcgtc agcttcgaaa gatcgaactt cactgacgtt tcttgaccac cgcccactac
      361 gttcgtgtgg gcgaggacgc gggcgtcgcc ggccggaaca taattcggag gaccggcagc
      421 ttgtccggca gcggcgactg cttggtaatc ggccgtggca gtgagcaccc agttgtgccc
      481 catcacgttc gctgcgagtt ggccgacgtg cttcagcgtg accgtgactt ctttgcagct
      541 cttgctgacg cgcagctctt tcttgttgaa ctgaatctgg tcgttccctt cgatcgatag
      601 ggaacactct tggctccatg cggtaccgct gagcaaaagc ccgcccagcg cgactaccca
      661 aaaggtcctt ctcattgtgg tttcggctcc tgaacgcaga atgcagaatg ggaactgcgc
      721 gatggtagcg aggttttgcg gtgcggcaca actgcaacgg gcaacgcacg gcgtgtgtaa
      781 cggcgacacc aactcggtgg ctccgcagcc gacacggctg cggccgcgcg acacgccgcc
      841 cacccggagc gcctcgacga tacgctcgtg cccgcgtctt gagccaatga gccaaaatag
      901 gcactcagtc ccactttagg acctaatgcg cgacatttcg agcgttctca tgggcaatag
      961 cgacagaata tcccaaaaat cgagcagcaa gccgtgatag ggctcatcca gcgcgtaagt
     1021 ggggcacaag tgaccgtgaa tgcgcaaaaa atcgcgcaaa ttgggcgagg tctgctcgtt
     1081 ttggtcggcg tcgagcgtga agatggcccc gagcaagccc aaaagctcgc ttcgaggctc
     1141 gtcgcgtacc gcgtattccc cgatgacgcc ggcaagatga acctgtccgt tcgcgaggtc
     1201 ggcggcgcgc tgttgctggt accgcagttc acgctcgccg cggacacgag cgcgggcctg
     1261 cgtccgagct tctcgcgcgc cgcgccgccc gaggtcggct cgcgtctgtt cgacgcgctc
     1321 gtcgccgccg tcgaggcgac gggagtgccg cacgctcgcg gccgcttcgg cgccgacatg
     1381 caggtcacgt tgacgaacga cgggcccgtg acgttttggc tgcaggcgta gagcaaccct
     1441 tcgtgccaaa tcgattggcg tatcattccg gctaacgcaa ggaagtcgac ggagctcacg
     1501 cgatgggatt tggcagcatc tggcactggc tgatcgtgct cgcgatcgtc gtgctgatct
     1561 tcgggacgaa gaagctgaag tcggcggccg gcgatatcgg cggcgccgtc aaggacttca
     1621 aaaaggcaat gagcgacgac gacaagccga gcgaggaagc gcagaagcgg ctcgagagcg
     1681 acgagcaaga cgccgaattt cccgagcaaa agaagaagaa caagaaccgc agtgcctgac
     1741 gctcggtgcc acgatgttcg acatcggtcc tggagagctc cttctcgtcg ccatcattgg
     1801 tctcctcgtt ttggggcctc agcgtctccc gaaagtcgcc gccgagatag gtaaatggga
     1861 cgggcgagcg cggcgcacgg cgtcgcagct gcggcgccag ctcgagcgcg agatcgagtt
     1921 gagcgagctc gagacgccgc cgcctgctcc gcctccgcct ccggcagcga cgccttcgcc
     1981 tgctcctgcc gcaacgaacg gcagcgggca ggcgacgact gacgcggaag cgggcgaggc
     2041 caatagcatc gggggccacg ccgcctcgag cgagccgtcg agcgccgcgg agaagcgcga
     2101 aggctgatga gcgcaaagcc cgacgagccc gaggagcagc tcgcggaagg gacgctcatg
     2161 tcccatttgc tcgagctgcg ctcgcgcatc atgaaggcgc tgctcgccgt gatcgtggtt
     2221 tttctcgttc tcgcgtactg gatgaacgac gtgttcgagc tcgtctcgaa gccgctgcgc
     2281 gacgcgctgc ccgaaggctc ggagatgatc gtgacggacg tcgccgctcc gttcctgacg
     2341 ccgatgaaaa ccgcgttctt cgtcgcgttg ttcatcgcga tgcccgtcgt cttgtttcag
     2401 gcctggcagt tcgtcgcgcc ggggctctac aagcgtgaaa aacgcctcgc gattccgctg
     2461 ctgctctcga gcatcgcgct cttctatgcc ggcatcgcct ttgcgtactt catcgtcttc
     2521 aaagtcatgt tcggattttt cgcgcgcgtc gtgccgggcg gcgtgcagat gtcgccggac
     2581 atcagcgctt acttgagctt cgtgctcggc gtttttctga cgttcgggct cgcgttcgag
     2641 gtgccgatcg cgaccgttct cctgatctgg agcggtctcg tctccgtcaa aacgctcaag
     2701 aacattcggc catacgtatt cctgggcgcg ttcgtcgccg gcatgctgct cgcgcccgac
     2761 gttttttcca tgacgttgat ggccatcccg atgtacgcct tgtacgaggc cgggctgctg
     2821 atggcgcgct tcctcgtgcc cgagaagatg gcggcggaga cggcgcccgc gcagaagtca
     2881 tgaggcgcat cgacagcaag ctgcccgccg tcggcacgac gatcttcacg ctgatgtcgc
     2941 agctcgccga gcagcacggc gccgtgaact tgagccaagg gtttcccgat tttcggccgc
     3001 cggccggctt gctcgagctc gtcgagcggt gcttacgcga acgcttccat cagtacgcgc
     3061 tgatgccggg ctcgatgccg ctgcgcaccg cgatcgcgca gaagctcgcc gcgatgtacg
     3121 acgtgacggt cgaccccgcc gccgagatca cggtcacgag cggcgcgacc gaggcgattt
     3181 tcgacgcgat tcaggccgtc gtgcgccgcg acgacgaggt catcgtgttc gatccgtgct
     3241 acgactcgta cgagccctcg atcatgctgg cgggcggacg cacgatccat ttgccgctga
     3301 cgcagcccga tttcgcgatc gattgggagc gattgcgggc cgcgctgagc cgccgcacga
     3361 ggctcgtgat cgtgaactcg ccgcacaatc cgagcggcgc gctgctcggc gccgcggact
     3421 tcgaccggct cgccgaggct ttgcggccct acgactgcta tctcctgagc gacgaggttt
     3481 acgagcacgt cgtcttcgac ggcgcccggc acgcgacggc gctcgcgcag cgcgagctca
     3541 aggagcgctg cttcgccgtg ttctcgttcg gcaagaccta ccacgccacg ggctggaagc
     3601 tcggctactg cgtcgcgccc gccgagctct cggccgagct gcggcgcgtg caccagtacg
     3661 tgaccttcgc ttcgacgtcg ccgctgcagc aggcgctcgc ggagtacatg acgcggcatc
     3721 ccgagcacca cgtcggcttg cccgcgttct atcaagagcg ccgcgactac ttcacggcgc
     3781 tgctcgagcc gacgaagttt cggctgcggc gcgcggccgg cacgtacttc cagctcgccg
     3841 actacggcgc gatctcgacg ctgcccgacg tcgagttcgc gcgctggctc acgatcgagc
     3901 acggcgtggc gacgattccg atctcggtgt tct
//
LOCUS       DASSNN010000202         4657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_544710, whole genome shotgun sequence.
ACCESSION   DASSNN010000202 DASSNN010000000
VERSION     DASSNN010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544710"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFL84_03705"
     CDS             <1..546
                     /locus_tag="VFL84_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET7607758.1"
                     /translation="VIPACENMPSGRATRPGDIVKSMSGQTVEIINTDAEGRLILCDA
                     ITYAQRFKPRCIIDVATLTGACVIALGHVYTGLFCNDEALAKSLLEAADRSLDLAWRL
                     PVHEEYGESLRSNFADFANSGTRDGGASVGANFLSRFVDGHAWAHLDIAGVAWRANAN
                     KGATGRPVPLLVDYLLNLDGQ"
     gene            543..977
                     /locus_tag="VFL84_03710"
     CDS             543..977
                     /locus_tag="VFL84_03710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HET7607759.1"
                     /translation="MTQVDFYILDSDSDDARLTLACKIVDKATQLDNHVYVHSASDDE
                     ARKLDELLWTFAQGSFIPHRVVRTALDAPPLEPVLIGVNQPPGPGRWDVLVNLATDVP
                     DFFSRYERVAEVVDANAARREQSRERYRFYRDRGYKLNTHQV"
     gene            1048..3810
                     /locus_tag="VFL84_03715"
     CDS             1048..3810
                     /locus_tag="VFL84_03715"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET7607760.1"
                     /translation="MNKDYRPAEIERRHYARWEAAGYFAPAGDGAPYCIVIPPPNVTG
                     TLHMGHALQDTIMDALTRYHRMQGRRTLWQPGTDHAGIATQMVVERLLEREGTSRAEL
                     GRARFLERVWQWKERSGNTIQQQLRRLGASVDWTRDRFTMDEGLSRAVTEVFVRLHEE
                     GLIYRGKRLVNWDPVLQTAVSDLEVLSTEERGKLWHLRYPLADAPDTHLVVATTRPET
                     MLGDTAVAVHPDDERYRHLVGKEVLLPLANRRIPIVADEYVDPEFGTGCVKITPAHDF
                     NDYEVGQRHDLPLISVLDFHGRLNENAPAAYRGLDRFEARKRIVADLEAQGLLERVTD
                     HTSMIPKGDRSGVVVEPMLTDQWYVRVKPLAEPAIAAVEDGRVKFVPENWSKTYFEWM
                     HNIQDWCISRQLWWGHRIPAWYDDEGAVYVGRDEADVRRKHGIPNERRLRQDEDVLDT
                     WFSSALWPFSTLGWPDDTAELRAFYPTNVLVTSFDIIFFWVARMLMMGLKFVGDVPFR
                     EVYIHALVRDHEGNKMSKSKGNILDPLDLIDGVDLPTLLKKRTDGLMQTHLKPGIEKA
                     TRKEFPHGIEGVGTDALRFTFATLATTGRDARFDVARAEGYHRFCNKLWNASAYVLSQ
                     LDGIDDGRYTLGIADRWIRSRLRATIASVHDNFALYRLDLIAQSLYDFAWYEFCDWYL
                     ELTKAVLNDPAADPALRRGAQQTLVEALGALLKLLHPLMPFVTEELWLEIVARRGFES
                     PTIMLERFPQQADFAEDAAAEDEIAWLKGFVGGIRQIRGEANLPRSATLNVRLADATP
                     LDRERVERHASQLRKLAGLDRIEFVPAGETVKGAATALLGAMRILVPLAGLIDVAAER
                     DRLGKQLAKTNDDLGKLRRKLDNQSFVANAPPDVVAKDQARVADLEQRASQLEQQIAR
                     LAELAS"
     gene            3965..4645
                     /locus_tag="VFL84_03720"
     CDS             3965..4645
                     /locus_tag="VFL84_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7607761.1"
                     /translation="MSHRAIAVIALLAISPAHAALIEYSFSGTLYWPYPSWPSSSYEA
                     FGVELGERITGGFLIDDAAPRTSYTEPRSGWSTSTYDMSDLRFWVAVGDHVLGATGTR
                     LTIADAAPDPWGSPYHTDRWILSMAGDGGEVNGYTVSSMSIDLLQFGSRPLTSSELQV
                     PDAAQWFSCCYPEVPVPFLVNFADGSRMAASGLSISVPEPGTLSLLAAGVLGALAARR
                     RRPVAGNV"
BASE COUNT          825 a         1587 c         1480 g          765 t
ORIGIN      
        1 gtgatccccg cctgcgagaa catgccgagc gggcgcgcga cgcgccccgg cgacatcgtc
       61 aagagcatgt cgggtcaaac cgtcgagatc atcaacacgg acgccgaagg caggctgatc
      121 ctctgcgatg cgattaccta cgcgcaacgg ttcaaaccgc gctgcatcat cgacgtcgcg
      181 acattgaccg gcgcgtgcgt gatcgcgctc gggcacgttt acaccgggct cttttgcaac
      241 gacgaggcgc tcgccaagtc gctgctcgaa gcggccgatc gctctctcga tctcgcgtgg
      301 cgcttgccgg tccacgaaga gtacggcgag tcgctgcgca gcaacttcgc cgacttcgcg
      361 aactcgggca cacgcgacgg cggagcgagc gtcggcgcca actttctgtc gcggttcgtc
      421 gacggccatg cgtgggcgca tctcgacatc gcgggcgtcg cttggcgcgc gaacgcgaac
      481 aaaggcgcca ccggcaggcc ggtgcctctg ctcgtcgact atctcctgaa tctggacggc
      541 cagtgacgca ggtcgacttc tacattctcg acagcgactc cgacgacgcg cgtctcacgc
      601 tcgcgtgcaa gatcgtggac aaggcgacgc agctcgataa tcacgtctat gtccattcgg
      661 cgtccgacga cgaggcgcgc aagctagacg agctcctctg gacgttcgcg caagggagct
      721 tcattccgca ccgcgtggtg cgcaccgcgc tcgacgcccc gccgctggag cccgtgctga
      781 taggagtcaa tcagcccccc ggccccgggc gctgggacgt gctcgtcaac ctcgccaccg
      841 acgtgccgga cttcttcagc cgctacgagc gcgtagcgga ggtcgtcgac gcgaacgccg
      901 cgcggcgcga gcaaagccgc gagcgctacc gcttttatcg agaccgcggc tataagctga
      961 acactcatca ggtatgagct cacgcgacgc gagcgacgcg gcgccgcgcg agcggcgtag
     1021 aacgcgcgac gaggcgcttg aacgaacgtg aacaaggact atcgacccgc cgagatcgaa
     1081 cgccgccact acgcgcgctg ggaagccgcg ggctatttcg cgccggcggg cgacggcgcg
     1141 ccgtattgca tcgtgattcc gccgccgaac gtcaccggca ccttgcacat ggggcacgcg
     1201 ctgcaggaca cgatcatgga tgccctgacg cgctatcacc gcatgcaggg ccgaagaacg
     1261 ttgtggcagc ccggcacgga ccatgccggc atcgcgacgc agatggtcgt cgagcggctg
     1321 ctcgagcgcg agggcacgtc gcgcgcggag ctcggccgcg cgaggttcct cgagcgcgtt
     1381 tggcaatgga aggaacgctc gggcaacacg atccagcagc agttgcgccg gctcggcgcc
     1441 tccgtcgact ggacgcgcga tcgcttcacg atggatgaag ggctgtcacg cgccgtcacc
     1501 gaagtgttcg tgcgcttgca cgaagaaggt ctcatctatc gcggcaagcg cctcgtgaat
     1561 tgggatccgg tgctgcaaac ggccgtgtcc gatctcgaag tcttgagcac cgaggagcgc
     1621 ggcaagcttt ggcatcttcg ttatccgctc gcggacgcgc cggatacgca cctcgtggtc
     1681 gcgacgacga ggcccgagac gatgctcggc gacacggccg tcgccgttca ccccgacgac
     1741 gagcgctatc gccacctggt cggcaaagaa gtgttgctgc cgctggcgaa ccgccgcatc
     1801 ccgatcgtcg ccgacgagta cgtggacccc gaattcggca cgggctgcgt caagatcacg
     1861 cccgcgcacg acttcaacga ctacgaggtc ggccagcggc acgatctgcc gctcatcagc
     1921 gtgctcgact tccatggcag gctcaacgaa aacgcgccgg ccgcttaccg cggtctcgat
     1981 cggttcgagg cgcgcaagcg catcgtcgcc gatctcgagg cgcaaggttt gctcgagcgc
     2041 gttaccgatc acacctcgat gatcccgaag ggcgaccgca gcggcgtcgt cgtcgagccg
     2101 atgctcacgg atcagtggta cgtgcgcgtg aagccgctcg ccgagcccgc gatcgcggcc
     2161 gtcgaggacg gccgcgtgaa gttcgtgcca gagaactgga gcaagaccta cttcgagtgg
     2221 atgcacaaca ttcaggactg gtgcatctcg cggcagctct ggtggggcca ccgcattccc
     2281 gcgtggtacg acgacgaggg cgccgtctac gtcgggcgcg acgaagccga cgtgcgccga
     2341 aagcacggca ttccgaacga gcgccgcttg cgtcaggacg aggacgtgct cgacacgtgg
     2401 ttctcgtcgg cgctgtggcc gttttccacg ctcggctggc ccgacgacac ggccgagctg
     2461 cgcgcgttct acccgaccaa tgttctcgtc acgagcttcg acatcatctt tttctgggtc
     2521 gctcgcatgc tgatgatggg gctcaagttc gtcggcgacg tcccgtttcg cgaggtctac
     2581 atccacgccc tcgtgcgcga tcacgaaggc aacaagatgt cgaagtcgaa gggcaacatc
     2641 ttggacccgc tcgatctgat cgacggcgtc gatctgccca cgctgctcaa gaagcgcacc
     2701 gacggcctca tgcagacgca tctcaagccc ggcatcgaga aagcgacgcg caaggagttc
     2761 ccgcacggca tcgaaggcgt cggcaccgat gcgctgcggt tcacgttcgc gacgttggcg
     2821 acgacggggc gcgatgcgcg cttcgacgtc gcgcgcgccg aaggctacca ccgcttctgc
     2881 aacaagctct ggaacgcgtc cgcgtatgtg ttgagccagc tcgacggcat cgacgacgga
     2941 cgctacacgc tcggaattgc ggaccgctgg atccgctcgc gactgcgcgc gacgatcgcg
     3001 tcggttcacg acaacttcgc gctctaccgt ctcgatctca tcgcgcagtc gctgtacgac
     3061 ttcgcctggt acgaattctg cgactggtac ctcgagctga ccaaagccgt gctcaacgat
     3121 cccgccgccg accccgcgct gcgccgcggc gcgcagcaga cgctcgtcga ggcgctcggc
     3181 gccctgctca agctgctgca tccgctgatg ccgttcgtga ccgaggagct ctggctcgag
     3241 atcgtcgccc gccgcggctt cgagagcccg acgatcatgc tcgagcgctt cccgcagcag
     3301 gccgatttcg ccgaggacgc cgccgccgag gacgagatcg cctggctcaa gggcttcgtc
     3361 ggcggcatcc gccaaatccg cggtgaagcg aacctgccac gctcggcgac gctgaacgtg
     3421 cggctcgcgg acgcgacgcc gctcgatcgc gagcgagtcg agcgtcacgc gagccagctc
     3481 cgcaagctcg cggggctcga ccgcatcgaa ttcgttcccg ccggcgaaac ggtgaagggc
     3541 gccgcaacgg cgctgctcgg cgcgatgcgc atcctggtgc cgctcgcagg tctcatcgac
     3601 gtcgccgccg agcgcgaccg cctcggcaag cagctggcca agaccaacga cgacctcggc
     3661 aagcttcgcc gcaagctcga caaccagagc ttcgtcgcga acgcgccgcc cgacgtcgtc
     3721 gcgaaagacc aagcacgcgt cgccgacctc gagcaacgcg cctcgcagct cgagcaacaa
     3781 atcgcccgcc tcgccgagct cgcttcatag aaagcggact gcagaattgg aaaaatttcc
     3841 tggaagcgcc gcgtgccggc acgcccctga gcgctggccg tcccacgaag aaaggcacta
     3901 aagcgcgccc actgcgcggc acgaaacttg ctgcgttccg aaatcgattc agggggtccc
     3961 aaccatgtct catcgagcga tcgcggtcat cgcgctcttg gctatttctc ccgctcacgc
     4021 agcgctgatc gaatattcgt tttcgggaac gctctactgg ccctacccta gctggccgag
     4081 cagctcgtac gaggcctttg gcgtagagct cggcgagcgc atcacaggcg gctttctaat
     4141 tgacgacgcg gcaccgcgga cctcttacac ggagccgcga agcggctggt cgacgtcgac
     4201 atacgacatg agcgatttgc ggttctgggt tgccgtcggc gatcacgtgc tcggggcgac
     4261 gggcactcgg cttaccatcg cggacgccgc gccggatcct tggggttctc cataccacac
     4321 cgatcgttgg atcctctcga tggccggcga cggcggcgag gtcaacggct ataccgtcag
     4381 ctcgatgagc atcgacctgt tgcagtttgg cagtcgcccg ctgacgagct ccgagcttca
     4441 ggtcccggac gcggcccagt ggttcagctg ttgttatccc gaagtaccgg taccgttcct
     4501 cgtcaacttc gcagacggct cacgcatggc cgccagcggg ctctcgatct ccgtgccgga
     4561 gccgggaacg ctctcgctgc tcgccgccgg ggtgctcggc gcgctcgcgg cacgacggcg
     4621 aaggccagtc gccggcaacg tctagcgcaa ttttctc
//
LOCUS       DASSNN010000203         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1426779, whole genome shotgun sequence.
ACCESSION   DASSNN010000203 DASSNN010000000
VERSION     DASSNN010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426779"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VFL84_03725"
     CDS             complement(<1..1142)
                     /locus_tag="VFL84_03725"
                     /inference="COORDINATES: protein motif:HMM:NF004767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HET7607762.1"
                     /translation="MAQPAAPLPSLEALDLEHMLHPNTNLAALHRGVPLVLARGKGVH
                     VWDNHGKQYIEAMAGLWCTTLGYGDDELARTAYEQIKKLSFSHLFTGKSHEPGILLAD
                     KLVKMAPFAASRVFFGNSGSDANDTQIKLDWYYNNALGRPKKKKIIARLKGYHGTTLG
                     SAGLTGLPSFHKLFDVPLPPFLHTDAPYYYRGAAPGESEDDYATRLAASLEQLILREG
                     PDTVAAFIAEPLMGVGGVLLPPRGYFPKIQAVLEKYDVLLIDDEVITGFGRTGELWGA
                     QAFGMRPQTLTAAKALSSAYLPISAVIVPELMYEPMVKSSGEVGLFGHGFTYSGHPVA
                     AAVALRALGIYEERKLYEHVRAIAPQFQAALKSLAAHPLVGDTRGIG"
     gene            complement(1206..>2009)
                     /locus_tag="VFL84_03730"
     CDS             complement(1206..>2009)
                     /locus_tag="VFL84_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607763.1"
                     /translation="WYQPFGPKGLYFYAPSLYLTSTQFNVYEGDQRITEAQLREGTLE
                     FAVGRELLTWGEYRFGVRAAQGAFDLRVGDPTQISEEKFRRGEFFSRFSVDTMDSVSF
                     PREGSQAVLEWRASRQRLFDADADFDQLLFSGTYAKTWGRHTVLTTLRWDTTVSGTAT
                     DDRLFRMGGFFDISGLNRNQLSGQHATRLGAAYYRRIGDLALFPAFAGMSLELGNVWD
                     SREDISSRDGILGGSFWAGVDTPVGPVYVGYGRAEGGEDAFYVSLGRVF"
BASE COUNT          355 a          688 c          657 g          309 t
ORIGIN      
        1 ccgatgccgc gcgtgtcgcc gacgagcgga tgcgccgcca aggacttcaa ggccgcttgg
       61 aactgcggcg cgatcgcacg cacgtgctcg tagagcttgc gctcctcgta gatgccgagc
      121 gcgcgcaagg ccacggcggc cgcgacggga tgccccgagt acgtgaagcc gtgcccgaac
      181 aagccgacct cgccgctcga cttcaccatc ggctcataca tcagctcggg cacgatgacg
      241 gcgctgatcg gcagatacgc cgacgacagc gccttggcgg ccgtcagcgt ctgcggccgc
      301 atgccgaacg cttgcgcccc ccaaagctcg cccgtgcggc cgaagcccgt gatgacctcg
      361 tcgtcgatga gcagcacgtc gtacttctcg agcacggcct gaatcttcgg gaagtagccg
      421 cgcggcggca gcagcacgcc gccgacgccc atcaacggct cggcgatgaa cgcggcgacg
      481 gtgtccggcc cttcgcgcaa gatcagctgc tcgaggctcg ccgcgagccg cgtcgcgtag
      541 tcgtcctcgc tctcgcccgg cgcggcgccg cgatagtaat aaggcgcgtc cgtgtgcagg
      601 aacggcggca gcggcacgtc gaacagcttg tggaacgacg gcaatcccgt gaggcccgcc
      661 gagccgagcg tcgtgccgtg atagcccttc aggcgcgcga tgatcttttt cttcttcggc
      721 cggcccagcg cgttgttgta ataccagtcg agcttgatct gcgtgtcgtt cgcgtccgag
      781 cccgagttgc cgaagaagac gcgcgacgcc gcgaacggcg ccattttgac gagcttgtcc
      841 gcgagcaaga tgccgggctc gtggctcttg cccgtgaaca aatgcgagaa cgacagcttc
      901 ttgatctgct cgtacgccgt gcgcgcgagc tcgtcgtcgc cgtagccgag cgtcgtgcac
      961 cagaggccgg ccatcgcttc gatgtactgc ttgccatggt tgtcccaaac gtggacgccc
     1021 ttgccgcgcg cgagcacgag cggtacgccg cggtgcagcg cggcgagatt cgtgttcgga
     1081 tgaagcatgt gctcgaggtc gagcgcctcg aggctcggaa gcggagcggc cggctgcgcc
     1141 atcgtcaatc tcgctctcga tgaaaggccg gagatgatac acccgcgcgg ccgcaaagcc
     1201 gggcgctaga acacgcgccc gagcgagacg tagaacgcgt cctcgccgcc ctccgcgcgg
     1261 ccgtagccga cgtaaacggg accgaccggc gtatcgacgc cggcccaaaa agagccgccc
     1321 aagatgccgt cgcgcgacga gatgtcctcg cgcgaatccc agacgttgcc gagctcgagg
     1381 ctcatccccg cgaaggccgg gaacagcgcg agatcgccga tgcgccggta gtacgccgct
     1441 ccgagccgcg ttgcgtgctg gcccgacagc tgattgcggt tcaaccccga gatatcgaag
     1501 aacccgccca tgcggaacaa gcgatcgtcg gtggccgtac cgctgaccgt ggtatcccag
     1561 cgcaacgtcg tgagcacggt gtggcgcccc caggtcttcg cgtacgtgcc ggagaacaag
     1621 agctggtcga agtcggcgtc ggcgtcgaac agtcgctggc gcgacgcccg ccattcgagc
     1681 acggcctgcg atccctcgcg cggaaacgag acgctgtcca tcgtgtcgac cgagaagcgg
     1741 ctgaagaact cgccgcgccg aaacttttcc tcggagatct gcgtggggtc gccgacgcgc
     1801 aagtcgaacg ccccttgggc cgcacgcacg ccgaaccggt actcgcccca ggtcaagagc
     1861 tcgcgcccga cggcgaactc gagcgtgccc tcgcgcagct gcgcttcggt gatgcgctga
     1921 tccccctcgt agacgttgaa ctgcgtcgac gtcagataca gcgacggcgc gtagaaatac
     1981 aaccccttcg gaccgaacgg ctggtacca
//
LOCUS       DASSNN010000204         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1788110, whole genome shotgun sequence.
ACCESSION   DASSNN010000204 DASSNN010000000
VERSION     DASSNN010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788110"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFL84_03735"
     CDS             complement(<1..182)
                     /locus_tag="VFL84_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607764.1"
                     /translation="MTASNRESAARRRSKAASPSICTSTDLRSTGAIMATYIVLSRFT
                     EQGLHSIKDTTKRADAV"
     gene            339..411
                     /locus_tag="VFL84_03740"
     tRNA            339..411
                     /locus_tag="VFL84_03740"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:372..374,aa:Phe,seq:gaa)
     gene            473..1102
                     /locus_tag="VFL84_03745"
     CDS             473..1102
                     /locus_tag="VFL84_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YajG family lipoprotein"
                     /protein_id="HET7607765.1"
                     /translation="MRDRFWHAPWLLAMVVPIGLAGCAFGTRHPTLVYPPDESPGYMA
                     LAHAEPNAPIAARHVRVVLHGFADERADKNVVGTVRNGYGVRTADVVPTNSVSDWVTA
                     AVRSELERSGYRVTRAETADDSGSYVVSGEILNVFCDMYLEFSGQVVLVATVRKDGRE
                     LLNKRYEGKGTSGIAVAATAESYAQSLARALASAIRPFVADVDRALISP"
     gene            complement(1115..1291)
                     /locus_tag="VFL84_03750"
     CDS             complement(1115..1291)
                     /locus_tag="VFL84_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607766.1"
                     /translation="MFVAILTARLASVYPPKESYIDVTTENDLKGRRRVRTARSAAAT
                     AAEGATAGRDMCEQ"
     gene            1566..>2008
                     /locus_tag="VFL84_03755"
     CDS             1566..>2008
                     /locus_tag="VFL84_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7607767.1"
                     /translation="MATSRQNGVFASARERAMYTSILVHLDDTPAGERRLGTAVATAQ
                     RFGAGLVAIYFVPAPPVEVDRAELAPPEISLRLSQGETRERVESMFRKRAKDAGVASV
                     DFRLLPTDRIDVAIAEMRCVDLSILGQYDPDRDRAGFGRRLAEQAV"
BASE COUNT          310 a          666 c          675 g          357 t
ORIGIN      
        1 actgcatcgg cgcgcttcgt cgtgtccttg atggagtgaa gaccctgctc ggtgaagcgc
       61 gacagcacga tgtaggttgc catgatcgct cctgtggacc ggaggtccgt ggaagtgcat
      121 atcgaggggc ttgctgcttt cgaacgacgc cgggccgcgg attctcgatt cgaggcggtc
      181 acgtggcgcc gtccgagcgt ccagcatagt ccgccgcggt cgcactttca aagatccgtc
      241 gctgggcgaa tcgacgccgc tcacgcggcg ggtggcggcg agcattgcta tacttcgcgt
      301 cccggccgga gcttcgactc cggccgctga gtttgcaagg ccaagtagct cagttggtag
      361 agcagaggac tgaaaatcct tgtgtcggtg gttcgattcc gcccttggcc acccaatact
      421 cgtacactgg gcagtcgtag cttggaaagc tcgccacgtt tcaggctgcc ctgtgcgaga
      481 tcgattctgg cacgcgcctt ggctgcttgc gatggtcgtc ccgatcggcc tcgcagggtg
      541 cgccttcggc acgcggcacc cgacgctcgt ctatccgccc gacgaatccc ccgggtacat
      601 ggccttggcg cacgcggagc cgaacgcgcc aatcgctgca cgccatgtgc gcgtcgtgct
      661 gcacggattt gcggacgagc gcgcggacaa gaatgtcgtc ggtaccgtca ggaacggcta
      721 cggcgtccgc accgccgacg tcgtaccgac gaacagcgta tccgactggg tgacggcagc
      781 ggttcgctcg gagctcgagc gtagcggtta tcgcgtcacg agggccgaaa cggccgacga
      841 cagcgggagc tacgtcgttt cgggcgagat cctgaacgtc ttttgcgaca tgtacctcga
      901 gttcagcggg caggtcgtgc tcgtcgccac cgtgcgcaag gacggcaggg aactgctcaa
      961 caaacgctac gagggaaagg ggaccagcgg catcgccgtt gcggcgactg ccgaatcgta
     1021 tgcgcagtcg ctggcacgcg ccctcgcatc cgccatcaga ccgttcgtcg ccgacgtgga
     1081 ccgcgcgctg atctcgccat agtgcgtttt tccgctactg ctcgcacatg tcgcgccccg
     1141 ccgtcgcccc ctcggcggcc gtcgcagccg ccgagcgggc ggtgcgcacg cggcgccgcc
     1201 ccttgagatc gttctccgtc gtaacgtcga tgtagctttc tttcggcggg tagacgctgg
     1261 ccagccgggc ggtcaggatc gcgacgaaca acgccccggt gatctgctcg atgatgcaga
     1321 cgccgcgcgc ctgccgcgtg agcggcgcgc aatgtccccg aatccggtgc tcgtgagcac
     1381 cgtcatgctg aggtagagcg cgtcggcgta gacgaggcgc cccgcctgtc cgacgatcat
     1441 gaacgactgc ggataaaggt agccgacgat cgcgtagttg taggccggta cgcggccgcg
     1501 gcgccgaaca gcttgtcctg cgcagggggc cgcggaggcc gtgttgacgt gcgtcaaccg
     1561 ccggcttggc gacgtcgcgg caaaatggcg tcttcgccag cgctcgggag cgcgccatgt
     1621 acacgtcgat tctcgtccac ctcgacgaca cgcccgccgg cgagcgtcgc ctgggcacgg
     1681 ccgtcgccac tgcgcagcgc tttggcgccg gcctcgtcgc gatctatttc gtgcccgcgc
     1741 cgccggtcga agtggaccgc gccgagcttg ctcctccgga gatctcgctt cgcctgtcgc
     1801 agggcgagac gcgggagcgc gtcgagtcca tgttccgcaa gcgcgccaag gatgccggcg
     1861 tcgcatccgt cgacttccgc ctgttgccga ccgaccggat cgacgtcgcg atcgccgaaa
     1921 tgcgttgcgt cgacctgtcg atcctcggcc agtacgatcc cgaccgggac cgcgcgggct
     1981 tcgggcgcag gctcgccgag caggcggt
//
LOCUS       DASSNN010000205         5417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_420914, whole genome shotgun sequence.
ACCESSION   DASSNN010000205 DASSNN010000000
VERSION     DASSNN010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420914"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFL84_03760"
     CDS             <1..357
                     /locus_tag="VFL84_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607768.1"
                     /translation="TTAAVAFLLLAPFAPLGAALMTTVASFTKTYREAQTYLTFVLLI
                     PTLPVAFATMLNIEPEPKLMWIPSLSQHLLVTTLIKDQPLAPAFVALSAASTLLFGVL
                     LGLLAVRLYRREAILG"
     gene            478..1059
                     /locus_tag="VFL84_03765"
     CDS             478..1059
                     /locus_tag="VFL84_03765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET7607769.1"
                     /translation="MSETPLAGTVQPPSFDALTLALTRPAFLAQLAEAAQIAARSGNV
                     FSLLLLDVDHLQNINDCHGVAAGDEVLAGLADRCRHVIAEPAWHRSEYAFGRYDGGAL
                     MVLARPCAISQAETLAEALRFAAAEKPLSGRISATVSIGVAQLRIGETLDELLARTER
                     VLHLAKQFGRDRVEVASAPPSRVERAKVVGLYD"
     gene            complement(1076..1360)
                     /locus_tag="VFL84_03770"
     CDS             complement(1076..1360)
                     /locus_tag="VFL84_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607770.1"
                     /translation="MERRIDRCLELCRHWAEGRVATGNEPPWAWYQYMKLIETVSAIQ
                     TSRAATTPSHEASPARERAAATRPRTDPVADIGAQRRRRCRDDEEPPLPM"
     gene            complement(1684..3027)
                     /gene="mnmE"
                     /locus_tag="VFL84_03775"
     CDS             complement(1684..3027)
                     /gene="mnmE"
                     /locus_tag="VFL84_03775"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011412233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET7607771.1"
                     /translation="MRRDDTIAALATPPGIGAVAVIRISGPNARAIALQLTGRDPTPR
                     VADLRTFRDASGATLDRGLVLFFPGPSSFTGEDVVELHCHGGRFVGDAVLAAAYRGGA
                     RPAEAGEFTLRAFLNDKIDLVQAEAIADLVASGSAQAARAAVRSLEGEFSSAVAELQH
                     ALTTLRVRIEAWLDFPDEELPFDAAPECTAELDALVAKLDTLCTRARSGRALRDGLSV
                     AIAGAPNAGKSSLLNRLAGYDAAIVTEVPGTTRDPLREHLSLDGLPLTVVDTAGLRDT
                     NDPVEREGVRRARLEVARADRLLWVADAREPLAATLAAARAAQAGNGAVTVIANKIDL
                     AGLEPKLRDERGTPVVYASALTGAGIELLVAHLKQAAGVVGEPSGTFSARRRHLDALE
                     RARAELGAARAFLTDALEIAAEHLRNAQTALSELTGELSSDDLLGEIFATFCIGK"
     gene            complement(3056..4747)
                     /gene="yidC"
                     /locus_tag="VFL84_03780"
     CDS             complement(3056..4747)
                     /gene="yidC"
                     /locus_tag="VFL84_03780"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET7607772.1"
                     /translation="MNNLNNIRLFLWLTLAGIAWLTYTAWNADYGRRAVTSPTPTEQT
                     SGAPSTDIPRASPGSGAAQPQVQAPQQQAAAATGELIHVRTDVLDLAITTQGGDLVRA
                     DLLRYPVDKDDPEHLVRLLDDSGPTRWVFQSGVSSAVGGAEPTHVQTFRSDSNEYVLA
                     PGQDELVVTLDWVGDGPLGARKTYTFRRGRYDISLNMTVAPSAGSIWQGTPYVRMERQ
                     HVPARRSYMSPDSYSFKGPVLYDGDAFERIKFEDLVRMPETRDVRGGWFASIQHHFLA
                     AAVPPSDAVYHYEATATGQYVDDELVGGPYVLTAVGPVTAASAAMPLSTTFTLFVGPK
                     LQKQLQEVGSDLERTVDYGITTPLAQPLFTVLDWVHGWVGNWGWAIIIVTILIKAAFY
                     KLTAMSGRSMAKMRKIAPRMKALQERYKDDREGLSKAMMELYKKEKVNPAAGCLPILI
                     QMPFFFAFYWVLVESVEMRQAPFALWLQDLSARDPFYVLPACMAAAMFFQTRMSPAPP
                     DPVQARMMQIMPLVFAAFLVVFPAGLVLYQLTNTTLSILQQWRINKLVARESAAT"
     gene            complement(4744..5112)
                     /gene="rnpA"
                     /locus_tag="VFL84_03785"
     CDS             complement(4744..5112)
                     /gene="rnpA"
                     /locus_tag="VFL84_03785"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET7607773.1"
                     /translation="MQPDTAGFSPDQRLHTPAEFGRVFAEPARSSDRFFTVLARPTGH
                     PAARLGLTVSRRAAKRAVDRNKIKRLARESFRHQPSLPPWDFVVLARAGADRAERRVL
                     RESLDRHFEQLTKAAAAQAR"
     gene            complement(5135..5269)
                     /gene="rpmH"
                     /locus_tag="VFL84_03790"
     CDS             complement(5135..5269)
                     /gene="rpmH"
                     /locus_tag="VFL84_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET7607774.1"
                     /translation="MKRTFQPSNLHRKRTHGFRARMATAAGRKVLASRRAKGRARLTP
                     "
     gene            5222..>5417
                     /locus_tag="VFL84_03795"
     CDS             5222..>5417
                     /locus_tag="VFL84_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607775.1"
                     /translation="MRALAVQIAGLKRSLHAEYPARVKSSCENKDVGKTGSRALYLAL
                     RGAVNREEPPLKREALAPDVV"
BASE COUNT          813 a         1860 c         1885 g          859 t
ORIGIN      
        1 acgacggccg ccgtcgcgtt cctgctgctc gccccgttcg cgccgctcgg cgccgcgttg
       61 atgacgaccg tcgcgtcgtt cacgaagacc tatcgcgaag cccagaccta cctcacgttc
      121 gtgctgctga tcccgacgct gccggtcgca ttcgcgacga tgctgaacat cgagcccgag
      181 ccgaagctga tgtggatccc gagcttgagc cagcatctgc tcgtgacgac gctgatcaag
      241 gatcagccgc tggcgccggc gttcgtcgcg ctctcggccg cgagcacgct tctcttcggc
      301 gttcttctcg gcctcctcgc cgtgcgcctc tatcggcgcg aagcaattct cggttaggaa
      361 gtggaacgag tttatgtaaa cgcggccctc gcgcttctgc gagagggttc acgccggctc
      421 gcgcatcggc gcggcaaagt gcttgcgtcg tccacgtatt tcgagatccg acgcccgatg
      481 tccgaaacgc cgctcgcagg aaccgttcag cccccctcgt tcgacgcgct gacgctcgcg
      541 ctgacgcgcc cggccttcct cgcccagctc gcggaagcgg cgcagatcgc ggcgcgcagc
      601 ggcaacgtgt tctcgctgct attgctcgac gtcgatcact tgcagaacat caatgactgt
      661 cacggcgtcg cggccggcga cgaggtgctc gcggggctcg cggaccgctg ccggcacgtc
      721 atcgccgagc cggcttggca tcgctccgag tatgcgttcg ggcgttacga cggcggggcc
      781 ttgatggtgc tcgcgcggcc gtgcgcgatc agtcaggccg agacgctggc cgaggcgctg
      841 cgattcgcgg cggccgagaa gccgctttcc ggtcgcatca gcgccacggt ctcgatcggc
      901 gtcgcgcagc tgcgcatcgg cgagacgctc gacgagctgc tcgcgcggac cgagcgcgtg
      961 ctgcatctcg cgaagcaatt cggccgcgac cgcgtcgagg tcgcgagcgc gccgccgagc
     1021 cgcgtcgagc gcgccaaggt cgtcggtctc tacgattagc ccgcctggta cctcgtcaca
     1081 tcggcagcgg gggctcttcg tcgtcgcggc accgccggcg ccgctgcgcg ccgatgtccg
     1141 ctaccggatc cgtcctgggg cgagtggcgg cggcacgttc gcgtgccgga ctggcttcgt
     1201 gcgacggcgt cgtcgccgca cggctcgtct ggatcgccga gaccgtctcg atgagcttca
     1261 tgtactgata ccaagcccac ggcggctcgt tgccggtcgc gactcgacct tcggcccagt
     1321 gtcggcagag ctccaagcat ctgtcgattc gcctttccat ggcagggctg tttcaagttc
     1381 catgccaacc atgcgccgcc gaaaaacaac gcgttgcgcg cggcagcgca ggcgcggtcg
     1441 caaatcttgt gagcgttgtc ggcgcacggc gccagccgtc gacgcgagat ttagcgcgct
     1501 agcgcagcgc cgagccggcg ggcacggggg cccgccgcgc ggcgaacgcg gccaagccgt
     1561 tcccacacgc gacgacctgt tcgagatacg tcgccatcag cggtagccgc cggtcggggg
     1621 aatcgcgtgg aggcatcgcg gccaatcgta ctactcgacg cagtcgcggc catctccggc
     1681 gcgctatttg ccgatgcaga acgtcgcgaa gatctcgccg agcaggtcgt cgctcgagag
     1741 ctcgccggtc agctccgaga gcgcggtctg cgcattgcgc agatgctcgg ccgcgatctc
     1801 gagcgcgtcg gtcaagaacg cgcgggccgc gccgagctcg gcgcgcgccc tctcgagcgc
     1861 gtcgagatgg cggcgccgcg cgctgaacgt gcccgacggc tcgccgacga cgccggccgc
     1921 ctgcttcagg tgcgcgacga gcagctcgat gccggcgccg gtcagcgccg acgcgtagac
     1981 gaccggcgtc ccgcgctcgt cccgcagctt cggctcgagg ccggcgagat cgatcttgtt
     2041 cgcgatgacc gtcacggcgc cgtttccggc ctgcgcggcg cgagcggccg cgagcgtcgc
     2101 ggcgagcggc tcgcgcgcgt cggcgaccca gagcaagcga tcggcgcgcg cgacctcgag
     2161 ccgggcgcgc cggacccctt cccgctcgac cgggtcgttc gtgtcgcgca ggcccgccgt
     2221 gtcgacgacc gtgaggggca ggccgtcgag cgacaggtgc tcgcgcagcg gatcgcgcgt
     2281 cgtcccgggc acctccgtca cgatcgcggc gtcgtaaccc gcgagccgat tcagcaggct
     2341 cgacttgccg gcgttcggcg cgcccgcgat cgcgacgctg agcccgtcgc gcagcgcgcg
     2401 ccccgagcgc gcgcgcgtgc agagcgtgtc gagcttcgcg acgagcgcgt cgagctcggc
     2461 cgtgcactcg ggcgccgcgt cgaacggcag ctcctcgtcg ggaaaatcga gccacgcctc
     2521 gatgcgcacg cgcaaagtcg tcaacgcatg ctgcagctcc gcgaccgccg acgagaactc
     2581 gccctcgagc gagcgcacgg ccgcgcgcgc cgcttgcgcg gagccgctcg cgacgagatc
     2641 ggcgatcgcc tcggcctgca cgagatcgat cttgtcgttc aggaacgcgc gcagcgtgaa
     2701 ctcgccggcc tccgcgggac gcgcgccgcc gcgatacgcg gccgcgagca cggcgtcccc
     2761 gacgaagcgg ccgccgtggc agtgcagctc gacgacgtcc tcgcccgtga acgagctcgg
     2821 gcccgggaag aacagcacga ggcctcggtc gagtgtcgcg ccgcttgcgt cgcggaacgt
     2881 gcgcaggtcg gcgacgcgcg gcgttgggtc gcgcccggtc agctgaagcg cgatggcgcg
     2941 cgcgttgggg cccgagatgc ggatgacggc gacggcgccg atgccgggcg gcgtggcgag
     3001 cgctgcgatc gtgtcgtcgc gccgcaatgc caagcgtgct ccccggaccg ttccgtcagg
     3061 tggctgccga ctcgcgcgcc acgagcttat tgatgcgcca ctgctgcagg atcgacagcg
     3121 tcgtgtttgt cagctgatac aacacgagac cggcggggaa caccacgagg aacgcggcga
     3181 acacgagcgg catgatctgc atcatgcgcg cctgcacggg atccggcggc gcggggctca
     3241 tgcgcgtctg gaagaacatc gcggcggcca tgcacgcggg cagcacatag aacgggtcgc
     3301 gcgccgagag atcttggagc cagagcgcga acggcgcttg ccgcatctcg acgctttcga
     3361 cgagcaccca gtagaacgcg aagaagaacg gcatctgaat caggatcggc aagcagcccg
     3421 ccgccggatt cactttttct ttcttgtaga gctccatcat cgccttgctg agcccttcgc
     3481 gatcgtcctt gtagcgctct tgcagcgctt tcatgcgcgg cgcgatcttg cgcatcttcg
     3541 ccatcgaccg tccgctcatc gccgtgagct tgtagaaggc ggccttgatc aggatcgtga
     3601 cgatgatgat cgcccagccc cagttcccga cccagccgtg cacccagtcg aggaccgtga
     3661 acagcggctg ggcgagcggc gtcgtgatgc cgtaatcgac cgtacgctcg aggtcgctgc
     3721 cgacttcctg cagttgcttc tgcagcttcg gcccgacgaa cagcgtgaac gtcgtggaga
     3781 gcggcatcgc cgcgctcgcg gcggtgacgg gcccgacggc cgtcaacacg tacggaccgc
     3841 cgacgagctc gtcgtcgacg tattggccgg tcgccgtcgc ttcgtagtgg tagaccgcgt
     3901 cgctcggcgg cacggcggcc gcgaggaagt ggtgctgaat cgacgcgaac cagccgccgc
     3961 gcacgtcgcg cgtctcgggc atccgcacta ggtcctcgaa cttgatgcgc tcgaacgcat
     4021 cgccgtcgta aagaaccggg cctttgaacg aatacgaatc cggggacatg taggaccggc
     4081 gggccgggac gtgctggcgc tccatgcgca cgtacggcgt gccctgccag atcgagccgg
     4141 ccgacggcgc gacggtcatg ttcagcgaaa tgtcgtagcg gccgcggcgg aacgtgtacg
     4201 tcttgcgcgc gccgagcggc ccgtcgccga cccagtcgag cgtgacgacg agctcgtcct
     4261 gccccggcgc gagcacatac tcgttgctgt cgctgcgaaa cgtttgtacg tgcgtcggct
     4321 cggcgccgcc gacggcgctc gacacgccgg actggaacac ccaacgggtc gggccgctgt
     4381 cgtcgagcag gcgcacgagg tgctcgggat cgtccttgtc gacggggtac cgcagcagat
     4441 cggcacgcac gaggtcgccg ccctgcgttg taatcgccaa gtcgaggacg tcggttcgaa
     4501 cgtggatcag ctcgccggtc gccgcggccg cctgctgctg cggcgcctgt acttgcggtt
     4561 gcgctgcccc cgagccgggc gacgcgcgcg ggatgtcggt cgacggcgct cccgaggtct
     4621 gctcggtcgg ggtcgggctc gtgacggcgc gcctgccgta gtcggcattc caggccgtgt
     4681 acgtgagcca cgcgatgccg gccagcgtga gccagaggaa caggcggatg ttgttgaggt
     4741 tgttcatcgc gcttgcgccg cggccgcctt cgtcagctgc tcgaagtgcc gatcgaggct
     4801 ttcgcgcagc acgcgccgct ccgctcgatc cgcgcccgct cgcgcgagca cgacgaagtc
     4861 ccacggcggc agcgacggct ggtggcgaaa ggactcgcgg gcgaggcgct tgatcttgtt
     4921 gcggtccacg gcgcgtttcg cagctcggcg cgagaccgtc aggccgagac gcgcagcggg
     4981 atggccggtc ggtcgagcga gcaccgtgaa gaagcgatcg ctcgagcgcg cgggctcggc
     5041 gaacacgcgc ccgaattccg cgggcgtgtg caatcgttgg tcgggtgaaa accctgccgt
     5101 gtcaggctgc aacggcgcgc caaaacgaaa tccgttaagg cgtgaggcga gcgcggcctt
     5161 tcgcgcgccg gcttgcgaga acctttcgac cggcggcggt ggccatgcgt gcgcggaatc
     5221 catgcgtgcg cttgcggtgc agattgctgg gctgaaacgt tcgcttcatg ctgagtaccc
     5281 cgcgcgggta aaaagttctt gcgaaaacaa ggatgtggga aagacgggaa gccgcgcact
     5341 gtacttggcc cttcgtggcg ctgtcaaccg cgaagagccg ccgctaaaac gcgaagcact
     5401 cgcacccgac gtcgtcg
//
LOCUS       DASSNN010000206         4311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_421110, whole genome shotgun sequence.
ACCESSION   DASSNN010000206 DASSNN010000000
VERSION     DASSNN010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421110"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..2558
                     /locus_tag="VFL84_03800"
     CDS             48..2558
                     /locus_tag="VFL84_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7607776.1"
                     /translation="MTGLLRRASLRFYLRHPWQLGLAIAGISLGVGVYVGVTLANDSA
                     ARAFDVTAAEVRGPLTHRLLPVDDGLDEHLYAELVLRDSAVNAAPAVEAEVRIAGRER
                     LRVPLMGLDPILGETAFRMAGVAPARGADLARLVAEPNTVLLPEDLARELGILSGDST
                     TLTIGSREATVRVIGLVARGGADVQSEPPIVADIATAQELRGTLGRIDRIDLELTEAQ
                     ARELKAATPSGTVLVAAENEDSAFRELTTAFRTNLTALGLLALVVGMFLIYGTMAFAI
                     LQRTQTLGILRTLGVSRGEILQTILWETAGIAALATALGLVLGHVLAIGLVGLVLQTI
                     GDLSFNAAVGAVEPTPWIYVQGAALGMVATLVAALKPALDAARITPAAALRRAVVERR
                     ARAAARHAAILALPILAASALILGFGPSHLGAGFAGLFGVLAAGALLTPFATVALMGA
                     LDRVLAGRAGIPVTLAVRGVSASLSRTGVATAALAVAVATVNGVGLMIASFRTSLDDW
                     LGTTLTADIYLSAAEDDGALSELVASGVLAQLRGVEGLTLTRTRIVPTAQGEVAIRAV
                     QPGARGFGLTLVAGDSGAAFEALADGTGVIASERLLFSSGLRVGDELELPTPEGTRRS
                     PVVGAFRDFNTGVPAVVMALDSYRRDWRDDVLTGVGIDLESGADEAAVEEAVRSLVGP
                     TTRLRSTSRLKELSLAVFDRTFKVTEVLRLLAGIVAFFGILSALLAIELERARELSVL
                     RTLGFTPAGLGATLLTQTGLLGIAAGLAAVPIGTALALLLVHVINRRSFGWTMDFVLT
                     PQALVSGVSLAVVAALLAGIYPAWRASRVELGAALRED"
     gene            2558..3700
                     /locus_tag="VFL84_03805"
     CDS             2558..3700
                     /locus_tag="VFL84_03805"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HET7607777.1"
                     /translation="MRRWFLVAAGIALVAACSDGVSPKLDAPTSSTGLRLLGTEAEDG
                     FARATEPRGFEFPADHGSHPEFRTEWWYFTGNLATAEGRHFGFELTFFRYALAPPSPT
                     PQATSAWRADQVWMAHFAITDTEGKRFVARERLTREALGLAGAAAEPLEIWVEDWSAT
                     GEARGDELRVRLGARDDVIALSLSLAATVPHVAQGNRGLDVKGAGTGNASHYYSVPRL
                     AADGEVNVAGETFSVTGVAWLDREWSTSSLDPGTVGWDWFALRLSNGSDLMFYRLRTA
                     AGEASPYSGGTLVTADGKRTPLGAEDVALTAIDQWTSRATGVRYPVAWRLAVPELGIE
                     LEIEPYLESQELALSVRYWEGAVHAAGRDSSGPLTAEGYLELAGYQ"
     gene            3873..4076
                     /locus_tag="VFL84_03810"
     CDS             3873..4076
                     /locus_tag="VFL84_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607778.1"
                     /translation="MLEWKVDPFRAKRMEQDPCARTYDFLNRLDHPELELADEPKKKT
                     APRRAMNPYDNAAPPAKRKKRPS"
     gene            complement(4140..4255)
                     /gene="rrf"
                     /locus_tag="VFL84_03815"
     rRNA            complement(4140..4255)
                     /gene="rrf"
                     /locus_tag="VFL84_03815"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          624 a         1412 c         1549 g          726 t
ORIGIN      
        1 gatcgcgtcg tgacgattcg cgccggccga atcgaggaag tgcggctgtg acgggcctgc
       61 tgcggcgcgc gtcgctgcgc ttctatctgc gccacccgtg gcagctcgga ctggcgattg
      121 cgggaatcag tctcggcgtc ggcgtctacg ttggcgtgac actcgcgaac gatagcgcgg
      181 cgcgggcgtt cgacgtcacg gcggccgagg ttcgggggcc gctcacgcac cgcttgctgc
      241 cggtcgacga tggtctcgac gagcatctgt acgccgagct cgttctgcgc gactcggcag
      301 tcaacgcggc cccggccgtc gaggcggaag tccgcatcgc gggacgcgaa agattgcgcg
      361 tgccgctcat ggggctcgat ccgattctcg gcgagactgc gtttcgcatg gcaggcgtgg
      421 cgccggcgcg gggcgccgat ctcgcgcgcc tcgtcgccga gccgaatacc gtgctcctgc
      481 ccgaggatct ggcgcgcgag ctcggcatct taagcggcga ctcgacgacg ctgacgatcg
      541 gaagccgcga agccacggtg cgcgtgatcg gcctcgtcgc gcggggcggc gcggacgttc
      601 agtcggagcc gccgatcgtc gccgacattg cgaccgcgca agagctgcgc ggaacgctcg
      661 gccgaatcga tcgcatcgat ctcgagctga ccgaagcgca agcgcgcgag ctcaaagccg
      721 cgacgccgag cggcaccgtg ctcgttgcgg ccgagaatga ggacagcgct ttccgcgagc
      781 tcacgacggc gttccgcacg aacctgacgg ccctcgggct cctcgcgctc gtcgtcggca
      841 tgttcctgat ctacggcacg atggccttcg cgatcctgca gcgcacgcag acgctcggca
      901 tcttgcggac actcggtgtc tcccgcggcg agatcttgca gacgattctc tgggagacgg
      961 ccggcattgc cgcgctcgcg acggcgctcg gcctcgtgct cgggcacgtt ctcgcgatag
     1021 gcctcgtggg cctcgtgctg caaacgatcg gcgatctttc gttcaacgct gccgtcggcg
     1081 ccgtcgagcc gacgccgtgg atctacgtgc aaggcgcggc gctcggcatg gtcgcgacgc
     1141 tggtcgcggc gttgaagccg gcgctcgatg ctgcgcgtat aacgccggcc gccgcgctgc
     1201 gccgagccgt cgtcgaacgg cgcgcgcgcg cggcagctcg gcacgccgca attctggcgc
     1261 tgccgattct cgccgcgagc gcgctgatcc tcggatttgg cccgagccac ctcggcgcgg
     1321 gtttcgccgg cttattcggc gtccttgcgg cgggagcgct gctgacgccg ttcgcgaccg
     1381 tcgcgctgat gggcgcgctc gatcgcgtgc tggcgggccg cgcgggaatt ccggtcacgc
     1441 tcgcggtgcg cggcgtcagc gcgtcgttga gccgcacggg cgttgccacg gccgcgctcg
     1501 cggtcgccgt cgcgaccgtc aacggagtcg ggctcatgat cgcgagcttc cgaacgagcc
     1561 tcgacgactg gctcggaacg acgttgaccg cggacatcta cttgagcgcg gccgaggacg
     1621 acggcgcact gtccgagctc gtcgcgtcgg gcgtcttggc gcagcttcga ggcgtcgaag
     1681 ggctcacgct gacgcgcact cgcatcgtgc ccacggcaca gggcgaagtc gcgatccgcg
     1741 ccgtgcagcc gggcgcccgc ggcttcggcc tgacgctcgt cgccggcgat agcggcgcgg
     1801 cattcgaggc gctggccgat ggaaccggcg tcatcgcgtc ggaacgactg ttgttctcga
     1861 gcggcttgcg cgtcggcgac gagctcgagc tgcccacgcc cgaaggaacc agacggtcgc
     1921 ccgtcgtcgg cgcgtttcgc gacttcaaca ccggcgtgcc ggcggtcgta atggcgctcg
     1981 attcttatcg ccgcgactgg cgcgacgacg tgctgacggg cgtcgggatc gatctcgaga
     2041 gcggcgcgga cgaagcggcc gtcgaggagg ccgttcgctc gctcgtcggg ccgacgaccc
     2101 ggttgcgctc gacctcgagg ctcaaggagc tgtcgctggc ggtcttcgac aggacgttca
     2161 aagtgaccga ggtgctgcgc ctgctcgccg gcatcgtcgc gtttttcggc atcttgagcg
     2221 cgctgctcgc gatcgagctc gagcgagcgc gggagctgag cgtgctgcgc acgctcggct
     2281 tcacgcccgc cggcctcggc gcgacgctgc tgacgcagac gggtttgctc ggaatcgcgg
     2341 ccgggctcgc ggccgtgccg atcggcacgg cgctcgcgct gctgctcgtg cacgtgatca
     2401 atcggcgctc gttcgggtgg acgatggatt tcgtgctgac tccgcaagcg ctggtgtcgg
     2461 gcgtatcgct cgccgtcgtc gcggcgctgc tcgcgggcat ctacccggcc tggcgcgcga
     2521 gccgtgtcga gcttggcgcg gcgctgcggg aggactgatg cgccgatggt tcttagtggc
     2581 ggcggggatc gcgctggtgg cggcgtgcag cgatggcgtg tcgccgaagc tcgacgcgcc
     2641 gacttcgagc acggggttgc ggctgctcgg gacggaagcc gaggacgggt tcgcgcgcgc
     2701 gacggaaccg cgcgggtttg agttcccggc cgatcacggc agccatcccg agtttcggac
     2761 cgagtggtgg tacttcaccg gcaacctggc gacggcggag ggtcggcatt tcggtttcga
     2821 gctcacgttc tttcgctacg cgctcgcgcc gccgtcgccc acgccgcaag ccacatcggc
     2881 atggcgcgcc gatcaggttt ggatggctca tttcgcgatc acggataccg aagggaagcg
     2941 cttcgtcgcg cgtgagcgcc tcacgcgaga agcgctcggt ctcgccggcg ccgccgcgga
     3001 gccgctcgag atctgggtcg aggactggtc cgcaaccggc gaggcgcgcg gcgacgagct
     3061 gcgcgttcgc ctcggggcgc gcgacgacgt catcgctctt tcattgagct tggcggcgac
     3121 ggtgccgcat gtcgcgcagg gcaatcgcgg cctcgacgtc aaaggcgccg gcaccggcaa
     3181 cgcctcgcac tattactcgg tgccgaggct ggccgcggac ggcgaggtca acgtcgcggg
     3241 cgagaccttc agcgtgaccg gcgtcgcttg gctcgaccgc gagtggagca cgagctcgct
     3301 cgacccgggc acggtcgggt gggactggtt cgcgctgcgc ctgtcgaacg gcagcgattt
     3361 gatgttctat cggctccgca cggcggcggg cgaggcgagc ccgtacagtg gcggcacgct
     3421 ggtcacggcc gacggaaagc ggacgccgct cggcgccgaa gacgtcgcat tgaccgcgat
     3481 cgatcaatgg acgagccgcg cgaccggcgt tcgctatccg gtcgcgtggc ggctcgcggt
     3541 ccccgaattg ggtatagagc tcgagatcga gccctacctc gagagccaag agctcgcgct
     3601 ctcggttcgc tattgggagg gcgccgtgca cgcggcgggt cgggattcga gtggcccttt
     3661 gacggcggag gggtatctcg agctcgcggg ctaccagtga tttcgcgggt tttgagacgt
     3721 gtcgccggtc gcgcacggca acgagttgtt aaagtggcga tggcctccgg tagggggacc
     3781 atttgacaaa acggttgtcg ttggcagaca tggaacgacg caagaagcct agaaaagatt
     3841 gggttctcgg tcaggacgaa gtcgggcgct cagtgctcga gtggaaagtc gacccgtttc
     3901 gcgccaagcg aatggaacag gatccgtgcg cgcgcacgta cgacttcctg aatcgcctgg
     3961 atcaccccga gctcgagctc gcggacgaac cgaagaagaa gaccgcaccg cggcgggcga
     4021 tgaatccgta cgacaacgcg gcaccgccgg ccaagcgtaa gaaacgacct tcgtagccgc
     4081 gctcgctgcc gcgcaaaata aaaaccccca gccttgcgac cgggggtttt gtattaaaag
     4141 cctggcgatg acctactctc gcgtgggatg accccacact accatcggcg ctggacgttt
     4201 tcacttccga gttcggaatg ggatcgggtg gttcccgctc gctatggccg ccaggcaaac
     4261 tgttcgaggt tgacgagccg gggctcgcgc cccggctcat ccgcctccaa t
//
LOCUS       DASSNN010000207         3721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1174686, whole genome shotgun sequence.
ACCESSION   DASSNN010000207 DASSNN010000000
VERSION     DASSNN010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174686"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..566
                     /locus_tag="VFL84_03820"
     CDS             267..566
                     /locus_tag="VFL84_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET7607779.1"
                     /translation="MIRRNGDGDFAMPLYEFKCRACGHRFDELVKVDETASCPKCGDA
                     RPERLFSTSAGVSTDRSRKRAAGVARRTAGKIKREKDHADAEYRRNYIKEHSEGG"
     gene            630..2060
                     /locus_tag="VFL84_03825"
     CDS             630..2060
                     /locus_tag="VFL84_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7607780.1"
                     /translation="MKSSRLLPLIVACALFVENMDSTVLSTSLPAIAADLATDPIALK
                     LALTTYLLALAVFIPVSGWVADRFGARSTFVSAIAVFLLGSIGCALSGSLGELVAGRF
                     VQGMGGAMMVPVGRLVLLRTIPKAELVQALSWVTIPALVGPMVGPPLGGFITTYFDWR
                     YIFLINLPIGALGMILALRFIPNAREQPPPLDWLGFALAGGGLGAAMFGFSTLGQHLI
                     PFELAAAALALGLAALVGYFFHERRQEHALLDLTLFRLATFRAGALGGLLFRIGIGAS
                     PFLLPLMLQLGFGLSPVQSGLITFVSSIGAIFMKTVAARTLNLLGFRRVLLWNALAAS
                     ALLCAFGLFRPSTPELVILATLFVSGCCRSLQFTSLNAITFADVENERMGQATSLSGM
                     LQQLSLSLGVAIGGYSLEIVGALTGRSSTELGNFTIAFLVVGLISAASAYYAWRLPRN
                     AGAEMAGRTRGARQAADPSTAELPAG"
     gene            2186..>3721
                     /locus_tag="VFL84_03830"
     CDS             2186..>3721
                     /locus_tag="VFL84_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607781.1"
                     /translation="MLSGVLQDLRIGARQLARNPGFTLIAVFSLAVGIGANTATFSFA
                     DGLMLRPLPLPDPSAVVTVGSINAATGGTNALRASYPDYVELRDATASFAGGLTAFED
                     IAVQVVASSGASPEIRTAELVSGNFFAAMGVQPALGRAFVPAEDSVPGRDAVMVLSHR
                     FWQRALGADASVLGRRLLVNGIEFTVVGVAPESFTGLDLFLRPDFYVPLMMWPALVGA
                     DQPNPLEQRDMRALELRGRLADSVAIEQASADVDRLGTALAEEYPATNRGFRVQVRTE
                     SENRSSENGFAAPAIGMLVVLGAVILIVACVNVAGLLTSRAPSRTGEISLRLSIGAGR
                     ARIVRQLLTESALLAAGGALAGALVGYLGMLLWRQIPIADDMAVELTFDVNRRVLLIN
                     LAVAAASVFLFGLMPALRTSRVSLTEALRAAGSTSAARMGWGRGALVVVQVALSVAFV
                     SVTAFIYTSFVDVVAKGPGVRTDGVLTMSFDTQLARYGPEEAQRFYERLADRARGVAG
                     VEAV"
BASE COUNT          498 a         1258 c         1264 g          701 t
ORIGIN      
        1 acctgcgtgc tcacgccgtc tcccttcggc atgggccttc cgtgcccgcg accagcagac
       61 gaggagtcgc gatgacgcgc caccggcgca ccttgcttcg gtcgtgcagc ggactcgacg
      121 ctctagcggc gcgttgcccc gcacgttctg gcccaacgag ggtatgctcg accagcgaag
      181 tccgctcggt ccaatcgacg ccaaacggcg acaatccgac gggaattcag ggcgacccga
      241 atgctatatt tgctgcagcc ggagccttga ttcgacgtaa cggcgacggc gactttgcga
      301 tgccgctcta cgaattcaaa tgccgcgcct gcggccaccg cttcgacgag ctcgtcaagg
      361 tggacgagac cgcgtcgtgc ccgaaatgcg gtgatgcaag gcccgagcgg ctgttctcga
      421 cgtcggccgg tgtcagcact gaccgcagcc gcaagcgcgc ggccggcgta gcgcgccgga
      481 ctgccggcaa gatcaagcgc gagaaggacc acgcggacgc ggagtacagg cgcaactaca
      541 tcaaggaaca ctccgagggc ggctgacgct ttccctgcgt ctgccggccg gccgcggcgg
      601 tcgattcgcg cgccttccgg atcttcgtca tgaaatcctc gcgccttcta ccgctgatcg
      661 tcgcttgcgc cttgttcgtc gagaacatgg actcgacggt cctctcgacg tcgttgccgg
      721 cgatcgccgc cgatctcgcg accgacccga tcgcgctcaa gctcgcgttg acgacgtatc
      781 tgctcgcgct cgccgtcttc atcccggtga gcggctgggt cgccgatcgt ttcggcgcgc
      841 gctccacgtt cgtgtccgcg atcgcggtgt tcctgctcgg ctcgatcggc tgcgcgctga
      901 gcggctcgct cggcgagctc gtcgcgggcc gcttcgtgca aggcatgggc ggcgcgatga
      961 tggtcccggt cgggcggctc gtgctgctca ggacgatacc gaaggccgag ctcgtgcagg
     1021 cgctttcgtg ggtcacgatt ccggcgctgg tcgggcccat ggtcgggccg ccgctcggcg
     1081 gcttcatcac gacctatttc gactggcgct acatttttct gatcaatctg ccgatcggcg
     1141 cactcggaat gattctcgcg ctgcgcttca ttccgaacgc acgcgagcag ccgccgccgc
     1201 tcgactggct cggcttcgcg ctcgcgggcg gcgggctcgg cgccgcgatg ttcggcttct
     1261 cgacgctcgg ccagcatttg atcccgttcg agctcgcggc ggcggcgctc gcgctcggcc
     1321 tcgcggcgct cgtcggctac ttctttcacg agcgccggca agagcacgcg ttgctcgacc
     1381 tcacgctgtt caggctcgcg acgtttcgcg ccggcgcact cggcgggttg ctgttccgca
     1441 tcggcatcgg cgcctcgccg ttcttgctgc cgctgatgct gcagctcggg ttcgggttga
     1501 gccccgtgca atcgggcctc atcacgttcg tctcgtcgat cggcgcgatc ttcatgaaga
     1561 cggttgcggc gcgcacgttg aacctgctcg gtttccgccg cgtgctgctt tggaacgcgc
     1621 tcgccgcgtc cgcgctcttg tgcgcgttcg gcctgtttcg gccgagcacg cccgagctcg
     1681 tgatcctcgc gacgctgttc gtgagcggct gctgccggtc gctgcagttc acgagcttga
     1741 atgcgatcac gttcgccgac gtcgagaacg agcgcatggg acaggcgacg agcctgtcgg
     1801 ggatgctgca gcagttgtcg ttgtcgctcg gcgtcgcgat cggcggttac tcgctcgaga
     1861 tcgtcggcgc gctcacgggg cgctcctcga ccgagctcgg caacttcacg atcgcgttct
     1921 tggtcgtcgg gctcatctcg gccgcctcgg cttactacgc ctggcgactg ccgcgcaacg
     1981 cgggcgcaga gatggccggc cgcacgcgcg gcgcgcgcca agcggcggac ccgagcacgg
     2041 ccgagctgcc ggcgggctag tgctgcttcc cgatctcggg acgggcgctg gccgatctcg
     2101 ggacgtcgcc ggtgtagcga tcgccgctga cccgcatgga cattggatcg gcgccttggc
     2161 acgccgattg ccttgcacaa gagcgatgct ctccggcgta ctccaagacc ttcgcatcgg
     2221 cgcgcggcaa ctcgcgcgca accccggctt cacgctcatc gcggtctttt ccctggcggt
     2281 ggggatcggc gccaatacgg cgaccttcag tttcgccgac ggactgatgt tgcggccgct
     2341 gcccctaccg gacccgagcg ccgtcgtgac ggtcggctcg atcaacgcgg cgacgggagg
     2401 cacgaacgcg ctgcgcgcgt cgtatccgga ctacgtcgag ctccgcgatg caacggcgag
     2461 ctttgcgggc gggctcacgg cgttcgagga catcgccgtg caggtcgtcg cgagctccgg
     2521 cgcatcgccg gaaattcgga ccgctgagct cgtgtccggc aacttctttg ccgcgatggg
     2581 cgtgcaaccc gcgcttgggc gtgcgttcgt ccccgcggaa gattcggtgc ccggccgcga
     2641 cgccgtgatg gtgttgagtc accgcttctg gcaacgggcg cttggcgcgg acgcctccgt
     2701 gctcggccgt cgattgctcg tgaacggaat cgagttcacc gtcgtgggcg ttgcgcccga
     2761 gagcttcacc ggcctcgatc tgttcttgcg gcccgatttt tacgtgccgc tgatgatgtg
     2821 gccggccctc gtcggcgccg atcagccgaa tccgctcgag caacgcgata tgcgcgccct
     2881 ggagctccgg ggacgattgg ccgattcggt cgcgatcgag caggcgtctg cggatgtcga
     2941 ccggctcggt actgccctcg ccgaggagta ccccgcgacg aaccgcggct tccgcgtgca
     3001 ggtgcgcacc gagagcgaaa accggtcgag cgagaacggg ttcgcggcgc cggcgatagg
     3061 aatgttggtc gtcctcggcg ccgtcatcct gatcgtcgcc tgcgtcaacg tcgccggatt
     3121 gctcacgagc cgcgctccgt cgcgaaccgg cgagatctcg ctacggcttt cgatcggtgc
     3181 cggccgggca cgcatcgttc gccagctgct cacggagagc gccttgcttg cggcgggcgg
     3241 cgcgttggcg ggtgcgctcg tcggctatct cggcatgctg ctctggagac agatcccgat
     3301 cgcggacgac atggccgtcg agctcacgtt cgacgtgaat cgacgtgtct tgctgatcaa
     3361 tctggccgtt gcagcggcga gcgtattcct gttcgggctc atgccggccc tgcggacctc
     3421 gcgcgtgagc ctcaccgagg cgttgcgcgc cgcggggagc acgtcggcgg cgcggatggg
     3481 gtggggccgt ggagccctcg tcgtcgtgca agtcgcgctg tcggtcgcgt tcgtctcagt
     3541 cacggcattc atttacacga gcttcgtcga cgtcgtggca aagggccccg gcgtgcgaac
     3601 agacggcgtg ctgacgatga gcttcgatac ccagctcgcg cgctacgggc ccgaagaagc
     3661 gcagcggttc tacgagcggc tcgccgatcg agcgcgcggc gtggcgggtg tcgaagcagt
     3721 c
//
LOCUS       DASSNN010000208         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_170493, whole genome shotgun sequence.
ACCESSION   DASSNN010000208 DASSNN010000000
VERSION     DASSNN010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170493"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFL84_03835"
     CDS             complement(<1..896)
                     /locus_tag="VFL84_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HET7607782.1"
                     /translation="MRRGLTGKYEATSISGDGVRAFVPAALPPSPPLDVGGGLQQQIE
                     SAVLALGRLDGVSTLLPDRELFLYAYVRKEAVLSSQIEGTESSLSDLLLFELDEAPGV
                     PLDDVVEVSNYVAALNHGLVRLREGLPLSNRLIREVHEVLLSRGRGSNKSPGEFRRSQ
                     NWIGGRRPSDATFVPPPHTAVADCMSALERFLHSDGDGLPILLRAGLAHVQFETIHPF
                     LDGNGRVGRLLITLLLCNAGVLREPLLYLSLYFKRNRARYYELLQMVREEGDWEAWLR
                     FFLDGVEEVADGSVTTAERLAE"
     gene            complement(1034..>2510)
                     /locus_tag="VFL84_03840"
     CDS             complement(1034..>2510)
                     /locus_tag="VFL84_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7607783.1"
                     /translation="VESVMMSVLGGVLGLGIGLLGVRWIDVATQPTGRPYWLEFTMDF
                     RVFAYFAAISIFTGILFGLAPALQVSKSNVSENLKEGGRSGSGKRAGRMTDALLVGQI
                     ALTIVLLVGAGLMIRSFLITQQFDIGVSTENLVSVQLQLPNARYPQPADRIAFQDRLH
                     QRLEAVPGVDALTIVSNPPAGGAQMLTLKIDGRDMVDANNRLPTVYRLVVVPDYFESL
                     ELPIRRGRGFGDADGAPGAEAVVVNEAFVAKYFPDTDPIGARLRLGQDVNRGTEDTAA
                     PWLTIIGVSPSVFQQSPQQDLSMQPLVYVPFRQEPPIGFTILARTRVAGNAVVDAVRN
                     ELRQLDADLPLFNIRTMDDILAQRNWPYRIFGTLFGAFAVIALLISAVGIYAVTAHGV
                     GQRTQEIGVRMALGADRRDILWLVLKQGTLRIVIGLVLGVLAAFGASRVFVSILVNTT
                     PTDPATFVSICLLLAAVTLLACFVPARRATQLHPTQALRVE"
BASE COUNT          490 a          814 c          806 g          400 t
ORIGIN      
        1 atttctgcga ggcgctccgc cgtcgtcacc gaaccatccg ccacttcctc gacaccgtcg
       61 agaaagaacc gcagccaggc ttcccaatcc ccttcctcac ggaccatctg cagcagctcg
      121 tagtagcgag ctcggtttcg cttgaaatag aggctgagat acagcaacgg ttcacgcagc
      181 acgccggcat tgcacagtag gagtgttatc agcaaacggc ctacccgtcc gttgccatcc
      241 aagaacggat ggatcgtctc gaactggacg tgcgcaaggc cggctcgcaa tagaatcgga
      301 aggccgtcac cgtcggaatg caagaaccgc tcgagcgccg acatgcaatc ggcaacggct
      361 gtatgtggcg gcggtacgaa cgtcgcatcg ctcggtcggc ggccgccgat ccagttttgc
      421 gagcgtcgga actcgcccgg actcttgttg ctgcctcgtc cgcgagagag gagtacttcg
      481 tggacctcgc ggatcaagcg attggatagc ggtagcccct cgcggagtcg cacgagacca
      541 tggttcaggg cggcgacgta gttcgaaacc tcgacgacgt cgtcgagcgg tacgccaggc
      601 gcttcgtcga gctcgaacag cagaagatcc gacagcgacg actccgtccc ctcgatctgc
      661 gacgagagca cggcttcttt tcgaacgtag gcgtacaaaa agagctcacg atccggaagc
      721 aacgtggaaa cgccgtccag gcgaccgagg gcaagcacgg cgctctcgat ctgttgctga
      781 aggccgccac cgacgtcgag cggtggagaa gggggaaggg ctgcgggaac gaacgcgcgg
      841 acaccgtctc cgctgatgct cgtggcttcg tatttgcccg tcagcccgcg acgcacgtgg
      901 gagccctcgt taagcaaact tgaatagccg gccgggaaat taagacttca ggtcataact
      961 ctaaatagcg cgcctaagcg gtgcaagtag ctccctaccc ggctcattac gcccgtcgga
     1021 tcgaggcgct gcgctattcc acgcgaaggg cctgcgtcgg atgcagctgc gtggcgcggc
     1081 gcgcgggcac gaagcaggcg agcagcgtga cggccgcgag cagcaggcag atcgagacga
     1141 acgtcgccgg gtccgtcggc gtcgtgttca cgaggatgga cacgaagacg cggctcgccc
     1201 cgaaggcggc gagcaccccg agcacgaggc cgatgacgat tcgcaaggtg ccctgcttca
     1261 ggaccagcca gagaatgtcg cgccggtccg cgccgagcgc catgcgcacg ccgatctcct
     1321 gcgtgcgctg accgacgcca tgggcagtca cggcgtagat gccgacggcc gagatcagca
     1381 gcgcgatgac cgcgaacgcg ccgaacagcg tcccgaagat gcggtacggc caattacgct
     1441 gcgcgaggat gtcgtccatc gtccgaatgt tgaacagcgg caggtcggcg tcgagctgcc
     1501 gcagctcgtt gcggaccgcg tcgacgacgg cgttgccggc cacgcgggtg cgcgcgagga
     1561 tcgtgaaccc gatcggcggc tcctgtcgga acggtacata gaccaagggc tgcatgctca
     1621 agtcctgctg cgggctctgc tgaaacacgg acgggctcac gccgatgatc gtgagccagg
     1681 gagccgccgt atcctccgtg ccgcgattga cgtcctgccc aagccgaagg cgagctccga
     1741 tcggatcggt gtcggggaaa tatttcgcca cgaacgcctc gttgaccacg actgcttcgg
     1801 cgcccggtgc gccgtccgcg tcgccgaatc cgcggccgcg tctgatcggc agctcgagcg
     1861 actcgaagta atcgggaacg acgacgaggc ggtagaccgt cggcagccgg ttgttggcgt
     1921 cgaccatatc gcgcccgtcg atcttcagcg tcagcatctg cgcgccgccg gcgggcggat
     1981 tcgacacgat cgtcagagca tcgacgcccg gcaccgcctc gagcctttgg tgcaatcggt
     2041 cctgaaacgc gatgcggtcc gccggctgcg gatagcgagc gttgggcagc tggagctgca
     2101 cgctgacgag attctcggtg ctcactccta tgtcgaattg ctgcgtgatc aagaagctgc
     2161 ggatcatcaa gcccgcgccg acgagcagca cgatcgtcag cgcgatctgc ccgaccagca
     2221 gcgcgtccgt catcctgccc gcgcgtttac cgctgccgct gcgaccaccc tccttcaggt
     2281 tctcgctgac gttcgacttc gagacctgca gcgccggcgc gaggccgaac agaatgcccg
     2341 tgaagatcga gatcgccgcg aagtacgcga acacgcggaa gtccatcgtg aactcgagcc
     2401 agtaaggccg ccccgtcggc tgcgtcgcga cgtcgatcca gcgcacgccg agaaggccga
     2461 tgccgagccc gagcacgccg ccgagaacgc tcatcatcac gctctcgacg
//
LOCUS       DASSNN010000209         3325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1788596, whole genome shotgun sequence.
ACCESSION   DASSNN010000209 DASSNN010000000
VERSION     DASSNN010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788596"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFL84_03845"
     CDS             <1..398
                     /locus_tag="VFL84_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET7607784.1"
                     /translation="LVVDDPMLRSTLGEEFASVPKEDDIAGTKRNMLSERVLDGEKYP
                     QIRITGTGPIMTGGMQELAVKVEMLGRVIDLEVPTAVTIDGDQLRAKGEFELNHADLG
                     MKPFTALAGALQVGETLSFTYDVTARRAP"
     gene            complement(422..607)
                     /locus_tag="VFL84_03850"
     CDS             complement(422..607)
                     /locus_tag="VFL84_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HET7607785.1"
                     /translation="MRDGLRKIWLLLREMTGDDAYERYLAHWRARHRADGAAPLDRSA
                     FYREEQRRKWDGVRRCC"
     gene            complement(612..2693)
                     /locus_tag="VFL84_03855"
     CDS             complement(612..2693)
                     /locus_tag="VFL84_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HET7607786.1"
                     /translation="MATTISQRWPRPVSLSVWALVVVLGTFALGVIAMARGESVDALW
                     FIVAAVCVYAVGYRFYSAFLAAKVFALDPTRATPAERFSDGRDFMPTNRWVVFGHHFA
                     AIAGPGPLVGPTLAAQFGYLPGTLWILIGAVLGGCVQDFVALFCSIRRDGRSLGQMAR
                     DELGPIGGAVALIGVMAIMIILIAVLGLVVVNAMRHSPWATSTVAATIPIAILIGLFM
                     RHLRPGRVLEGTAIGVVLLLVAVFAGGWVDDHPTLRAWFDYDAPDLALMVILYGFAAA
                     VLPVWLLLAPRDYLSTFMKIGTIVALAVAILILNPRVQMPALTQFVDGTGPIFGGNVF
                     PFVFITIACGAISGFHSLIASGTTPKLLANERDARLIGYGAMMMESFVAVMAMVAACV
                     LDPGVYFAINSPGGVVGADPATAVATISSWGFPVTAEQMSTLANQMGESTLFARTGGA
                     PSLAVGMASIFSNAFGNTLLSVWYHFAIMFEALFILTTVDAGTRVARFMLQDLLGNVV
                     RPLGRTSWYPSVLLCSAIVVAAWGYFLYFGTIDPLGGINSLWPLFGIANQMLAAIALC
                     VATTVLVKSARARFAWVTVLPLVWLVAITTVAAWDKLFSPDLRVGFLSHANDLAAKLD
                     AGSLTGAAAAQAPQLIFNDRLDAALTFFFLVTVWVLVVETARICHACLSGRRCPPLSE
                     TPHIPTRLVEG"
     gene            2858..>3325
                     /locus_tag="VFL84_03860"
     CDS             2858..>3325
                     /locus_tag="VFL84_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HET7607787.1"
                     /translation="MSEPLPFAKAGAAQISLLPALANRHGLITGATGTGKTVSLQTLA
                     EQFSRIGVPVFMADVKGDLAGLAAAGGANAKVAERAKTLGVELRYEACPVVFWDVFGE
                     RGHPIRATVSDLGPLLLARLLQLNDTQEGVLSLTFKVADDAGLLLLDLKDLRAM"
BASE COUNT          628 a         1080 c         1150 g          467 t
ORIGIN      
        1 agctcgtcgt cgacgacccg atgctacgca gcacgctcgg cgaggagttc gcgagcgtgc
       61 cgaaggaaga cgacatcgcc ggcacgaagc gcaacatgtt gagcgagcgc gtgctcgacg
      121 gcgagaagta tccgcagatc cgcatcacgg gcaccggtcc gatcatgacg ggcggcatgc
      181 aggagctcgc ggtcaaggtc gagatgctcg ggcgcgtcat cgatctcgag gttccgaccg
      241 cagtgacgat cgacggcgat cagctgcggg cgaaaggcga attcgagctc aatcacgccg
      301 atctcggcat gaagccgttc acggccctgg cgggcgcgct gcaagtcggc gaaacgctgt
      361 cgttcaccta cgacgtgacg gctcgccgcg cgccctgaga aaagggggcg catttccttt
      421 tttagcagca gcgcctcacg ccgtcccact tccgccgctg ttcctcgcga tagaacgccg
      481 atcgatcgag cggcgccgcg ccgtccgcgc ggtgccgagc gcgccaatgc gcgaggtagc
      541 gctcgtaggc gtcgtcgccc gtcatttcgc gaagcagcag ccaaatcttg cgaagcccgt
      601 cccgcatggc gtcagccctc gacgagccgc gtcgggatgt gcggcgtctc cgacagcggc
      661 gggcagcggc ggccggacag gcacgcgtgg cagatgcggg ccgtctcgac gacgagcacc
      721 cagaccgtga ccaagaagaa aaaggtgaga gcggcgtcga gccggtcgtt gaaaatcagc
      781 tgcggcgcct gagcggccgc cgcgccggtc agcgagccgg cgtcgagctt cgcggccagg
      841 tcatttgcgt gcgacaggaa cccgacgcgc aggtccgggc tgaagagctt gtcccaagcc
      901 gcgaccgtcg tgatggcgac gagccagacg agcggcagca cggtgaccca ggcgaaccgg
      961 gcgcgcgccg atttcacgag cacagtcgtc gcgacgcaga gcgcgatcgc cgcgagcatt
     1021 tgattcgcga tgccgaacag cggccagagg ctgttgatgc cgccgagcgg atcgatcgtg
     1081 ccgaaataga ggaagtagcc ccaagccgcg acgacgatcg cgctgcagag cagaacgctc
     1141 gggtaccagc tcgtgcggcc gagcggacgc acgacgttgc cgagcaagtc ttggagcatg
     1201 aagcgcgcga cgcgcgtgcc ggcgtcgacg gtcgtcaaga tgaacagcgc ctcgaacatg
     1261 atcgcgaagt ggtaccagac cgacagcaac gtgttgccga acgcgttgga gaagatgctc
     1321 gccatgccga cggccagcga cggtgcgccg ccggttcgcg cgaacagcgt gctttcgccc
     1381 atctggttcg cgagcgtgct catctgctcg gccgtgacgg ggaagcccca cgagctgatc
     1441 gtcgcgacgg cggtcgcggg gtccgcgccc accacgccgc cgggactgtt gatcgcgaaa
     1501 tagacgccgg ggtcgagcac gcacgcggca accatcgcca tgaccgcgac gaaggactcc
     1561 atcatcatgg cgccgtagcc gatcaagcgc gcgtcgcgct cgttggcgag cagcttcgga
     1621 gtcgtccccg aggcgatcag cgagtgaaaa cccgagatcg cgccgcaggc gatcgtgatg
     1681 aacacgaacg gaaacacgtt gccgccgaag atcggtcccg tgccgtcgac gaactgcgtc
     1741 agcgcgggca tctgcactcg cggattcaat atgagtatgg cgacggccag cgcgacgatc
     1801 gtgccgatct tcatgaacgt cgagagatag tcgcgcggcg cgagcagcag ccataccggg
     1861 agcacggccg ccgcgaaccc gtacaagatc accatcagcg cgaggtccgg cgcgtcgtag
     1921 tcgaaccacg cgcgcagcgt gggatggtcg tcgacccagc cgccggcgaa cacggcgacg
     1981 agcagcagca cgactccgat cgccgtgcct tcgagcacgc gcccgggccg caggtggcgc
     2041 atgaagagac cgatcaagat cgcgatcgga atcgtcgccg cgacggtcga ggtcgcccat
     2101 gggctgtgcc gcatcgcgtt cacgacgacg aggccgagca ccgcgatcaa gatgatcatg
     2161 atcgccatga cgccgatcag cgcgacggcg ccgccgatcg gcccgagctc gtcgcgtgcc
     2221 atctgcccga gcgagcggcc atcgcggcgt atcgagcaga acagcgcaac gaagtcttga
     2281 acgcagccgc cgagcacggc tccgatcagg atccagagcg ttccgggcag atagccgaac
     2341 tgagcggcca gcgtcgggcc gaccaagggc ccgggaccgg cgatggcggc gaagtgatgg
     2401 ccgaacacga cccagcggtt cgtcggcatg aagtcgcggc cgtcgctgaa cctttcggcg
     2461 ggcgtcgcgc gcgtcgggtc cagcgcgaaa actttcgcgg cgaggaaagc gctgtagaag
     2521 cggtagccga ccgcatagac gcaaacggcc gcgacgatga accagagcgc gtcgacgctc
     2581 tcgccgcgcg ccattgcgat gacgcccaag gcgaaggtgc cgagcaccac caccaaggcc
     2641 cacacggaga gcgagaccgg ccgtggccag cgttgtgaga tcgtggtcgc catcgcgacg
     2701 gagcccccca gcattcgagt acgagcggcg gcggccgcga attgctttcg cggcgctgcc
     2761 gccgtctatg gcagcgtaag ctaccgtaga aaaggcggtg ttgtatattc cgggagcgcg
     2821 agcgcgactg ggcgagcgcg gcgcagaggt tcatcgcatg agcgagccgc ttccgttcgc
     2881 gaaggccggc gccgcgcaga tttcgttgct gccggcgctc gcgaatcggc atggcctcat
     2941 caccggcgca acgggcaccg gcaagaccgt gtcgttgcaa actctcgccg agcaattctc
     3001 gcgaatcggc gtgcccgtgt tcatggccga cgtcaaaggc gacctcgcag ggctcgcagc
     3061 ggccggcggc gccaacgcga aggtcgcgga gcgcgcgaag acgctcggcg tcgagcttcg
     3121 ctacgaagcg tgtcccgtcg tcttctggga tgtgttcggc gagcgcggtc atccgattcg
     3181 ggcgacggtg tcggacttgg ggccgctgct gcttgcccgc ctgctgcagc tgaacgacac
     3241 gcaggaaggc gtgctgagtc tcaccttcaa ggtcgccgac gacgcggggc tcttgctgct
     3301 cgatctcaag gatctgcgcg cgatg
//
LOCUS       DASSNN010000210         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_41241, whole genome shotgun sequence.
ACCESSION   DASSNN010000210 DASSNN010000000
VERSION     DASSNN010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41241"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VFL84_03865"
     CDS             <1..420
                     /locus_tag="VFL84_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607788.1"
                     /translation="LPTPAGETRPDPKNARGYSGQWSCLEQFKSGLLYFVSRIRDRRY
                     VAVDQERGVVAVFAFFDHMAGSTRNFTRPDGTPTTAGPAQPWTWEILEIFKVENNQLA
                     EIEAFLTRPPYGMLSGWSTWEDGMSDKIQDVTGYRER"
     gene            complement(431..577)
                     /locus_tag="VFL84_03870"
     CDS             complement(431..577)
                     /locus_tag="VFL84_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607789.1"
                     /translation="MAARLASLAVGLAVARVLKADERELVVEFEDGSRLLVRADERLD
                     ISVT"
     gene            970..1458
                     /locus_tag="VFL84_03875"
     CDS             970..1458
                     /locus_tag="VFL84_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008097671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HET7607790.1"
                     /translation="MLKTGALIAACALLAACDRQEPAAPAAPAAPSAAAPAAAPAAPT
                     RKPAPPGAMAYIIEPADGAKVTNPVRVVFGLKGFGVAPAGVERNDAGHHHLLIDAAMP
                     ANLALPLPNDETHRHFGGGQTEVELTLTPGRHTLQLVLGDHLHIPHDPPIASSVVTIE
                     VQ"
     gene            1517..3172
                     /locus_tag="VFL84_03880"
     CDS             1517..3172
                     /locus_tag="VFL84_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7607791.1"
                     /translation="MYSEFFGLNEKPFAITPDPRYLYMSARHTDALAHLLYGISESGG
                     FIQLTGEVGTGKTTLIRSVLEQLPAKADIALILSPQLTTLEFLATIVQELRCPQPADR
                     TVKAHIDVLNSHLMQAHAEGRRVVLIVDEAQTLSPELLEQVRLLTNLETSKQKLLQII
                     LIGQPELRELLDRPEMRQIAQRITGRYHLEPLRKDETRAYVSHRLRVAGTQNDIFTKR
                     AIDALYKHARGIPRLINVIADRALLAAYTKDQRSVDAKLVSAAASEVFGIRRKAPWWP
                     VATAMGGAAAIVLGVTNLGQAPAVREAPQEQFLGAPASPPSQTTALAVDANAAPAPHE
                     PAAAATAEPPTLASLLKDPTFALTTDGAIAELLSVWGAPYDPARGEPCIQAQERGLRC
                     LYQRRGTLSELRRVNWPAVLSLVTPDGTEQPVVVTALGYDNAQLAANGSTFELPLAEL
                     SYYWFGDHLLLWRPGDAPARDLTPGVDDDGVRWLRETLARLAGEPAAAPLSTRYDDAL
                     EQRVRAYQRAHQLTVDGIVGARTQIAMLAELGLPDTPSLVKDH"
     gene            3174..>3416
                     /locus_tag="VFL84_03885"
     CDS             3174..>3416
                     /locus_tag="VFL84_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607792.1"
                     /translation="MSFILDALRKSEHERQRSATPGLTQVPLATPEPQMPRWALGVIG
                     ILVAAVLVLGGAWWQSTRVPAEVAASSAPPEASVPTV"
BASE COUNT          566 a         1187 c         1139 g          524 t
ORIGIN      
        1 ctgccgacgc ctgcgggtga aacgcgtccg gatccgaaga acgcgcgagg ttactcgggc
       61 caatggtcct gcctcgagca gttcaagtcg ggactgctct acttcgtctc gcgcattcgc
      121 gaccgccgct acgtcgcggt cgatcaagag cgcggcgtcg tcgccgtgtt cgcgttcttc
      181 gatcacatgg ccggcagcac gcgcaacttc acgcggcccg acggcacgcc gacgacggcc
      241 gggcccgcgc agccgtggac ctgggagata ctcgagatct tcaaggtcga gaacaaccag
      301 ctcgccgaga tcgaggcgtt cctgacgcgg ccgccctatg ggatgctgtc cggctggagc
      361 acgtgggaag acggcatgtc ggacaagatt caggacgtga ccggctaccg cgaacggtaa
      421 gtctcgtcga ctatgtaacg gagatgtcca gccgctcgtc ggctctgacg agcaagcgcg
      481 acccgtcctc gaactcgacg acgagctcgc gctcgtcggc cttgagcacg cgagcgaccg
      541 cgaggccaac cgcgagactc gccaaccgcg ctgccaagtc ggcaggcttc gctttcgctt
      601 tccgagctct cgcggacgcg gccatcacag ttcctccttg aggcgaccgt cgcaccaaac
      661 cgaccgatcg cgcacacctc gctcgaagtc cttcgccgtc ttatacacct ccgaatggtc
      721 ggcatgtagc atgtccagct gccagatttc tccggtctca cgatgacgcc gcgcgcggcg
      781 accgcgaacg ggaccgaata gctctgcgcg tgtatcgggt gtgtcctcca tccgcgccga
      841 actttggacc cgcgcgatag gcgcgggcga tgcgccgatc cagccagagt tggtagtttc
      901 tgtccggggt cgagcaccgg aacggctgtg ctagagttgg gcgaaaacga gatgcggggg
      961 aaactgccca tgctgaaaac cggcgctctg atcgctgcct gcgcgctgct cgcggcctgc
     1021 gaccggcaag aaccggccgc accggcggct ccggccgcgc cgtccgcggc cgcacccgcc
     1081 gcggcgccag ccgcgccgac gcgcaagccc gcaccgccgg gcgcgatggc ctacatcatc
     1141 gagcccgcgg acggcgccaa agtcacgaat cccgtgcgcg tcgtgttcgg tttgaagggc
     1201 ttcggcgtgg cgccggccgg cgtcgagcgc aacgacgcgg gccatcatca cctgctcatc
     1261 gacgcggcga tgccggcgaa tctcgcgctg ccgctgccga acgacgagac gcaccggcat
     1321 ttcggcggcg gacagaccga ggtcgagctc acgttgacac cgggccgtca cacgctgcag
     1381 ctcgtgctcg gggatcacct gcacattccg cacgatccgc cgatcgcgtc gagcgttgtg
     1441 acgatagagg ttcaatagcg ggcgaacgcc ggcccgcacg cgcggtcgaa cgcgtaggct
     1501 ggccttagag cgcaacgtgt actccgagtt tttcgggcta aacgagaagc ctttcgcgat
     1561 cacgccggat ccgcgctatc tctacatgag cgcgcggcat acggatgcgc tcgcgcatct
     1621 tttgtacggc atctccgaaa gcggcggttt catccagctc acgggcgagg tcggcacggg
     1681 caagacgacg ctgatccgca gcgtgctcga gcaactgccc gcgaaagcgg acatcgcgct
     1741 gatcctttcg ccgcagctca cgacgctcga gttcttggcg acgatcgtgc aggagttgcg
     1801 ttgcccgcag ccggccgacc gcactgtcaa agcgcacatc gacgttttga actcgcacct
     1861 gatgcaagcg cacgcggagg gccggcgcgt cgtgctgatc gtcgacgaag cgcagacgct
     1921 ttcgccggaa ttgctcgagc aagtgcgcct gctcacgaac ctcgaaacgt cgaagcagaa
     1981 gctgctgcag atcatcttga tcggccagcc ggagctgcgc gagctgctcg atcgtcccga
     2041 gatgcgccag atcgcgcagc gcatcacggg gcgttatcac ctcgagccgc tgcgcaagga
     2101 cgagacacgc gcgtacgtga gccatcggct gcgtgtcgcg ggaacgcaga acgacatctt
     2161 cacgaagcgc gcgatcgatg cgctctacaa gcacgcgcgc ggaatcccgc gcctcatcaa
     2221 cgtcatcgcc gacagagcgc tgctcgcggc gtacacgaaa gatcagcgca gcgtcgacgc
     2281 gaagctcgtc tcggcggcgg cctccgaagt attcggcatc cggcgcaaag cgccgtggtg
     2341 gccggtcgcg accgcgatgg gcggcgccgc ggcgatcgtg ctcggcgtca cgaacttggg
     2401 acaagcgccg gccgtgcgcg aggctccgca agagcagttt ttgggcgcgc cggcctcgcc
     2461 cccttcgcaa acgaccgcct tggccgtcga cgcgaacgcc gcgccggcgc cgcacgagcc
     2521 ggccgcggcg gcgaccgcgg agccgcccac gctcgcgtcg ttgctgaaag acccgacgtt
     2581 cgctctgacg accgacggcg cgatcgccga gctgctctcg gtctggggag cgccttacga
     2641 ccccgcgcgc ggcgagccgt gcatccaggc gcaggaacgc gggctgcgct gcctctatca
     2701 acgccgcggc acgttgagcg agctgcgtcg agtgaactgg cccgccgtgc tgtcgctcgt
     2761 gacgccggac ggaacggagc agcccgtcgt cgtcaccgcg ctcggctacg acaacgcgca
     2821 gctcgccgcg aacggcagca cgttcgaatt gccgctcgcg gagctcagct actactggtt
     2881 cggcgatcac cttttgttgt ggcggcccgg cgatgcgccg gcgcgcgacc tgacgccggg
     2941 cgtcgacgac gacggcgttc gctggctgcg cgagacgctc gcgaggctcg ccggcgagcc
     3001 ggccgccgct ccgctctcga cccgctatga cgacgcgctc gagcagcgag tgcgcgcgta
     3061 tcagcgcgcg caccagctca cggtcgacgg catcgtcggc gcgcgcacgc agatcgcgat
     3121 gctggcggag ctcggcctgc cggacactcc gtcgctcgtg aaggaccatt gacgtgtcgt
     3181 tcatcctcga cgcactgcgc aagtccgagc acgagcggca acgctcggcg acgccgggcc
     3241 tcacgcaagt gccgctcgcg acgccggagc cgcagatgcc gcgatgggcg ttgggcgtga
     3301 tcggtatcct agtcgcagcc gtgctcgtgc tcggcggcgc atggtggcag agcacgcgcg
     3361 tgccggccga ggtcgccgcg tcgtcggcgc cgccggaggc gtcggtcccg acggtc
//
LOCUS       DASSNN010000211         5326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1284311, whole genome shotgun sequence.
ACCESSION   DASSNN010000211 DASSNN010000000
VERSION     DASSNN010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284311"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VFL84_03890"
     CDS             complement(<1..1102)
                     /locus_tag="VFL84_03890"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET7607793.1"
                     /translation="MSGAAMPLPSFEILAAALRRTTERLASELAEPRESEPDWNEVEW
                     AVARSAAAMQGISTLLKNKLVWPGPPAWQAFLADQHGHSVLRHERIGALLGEIDTALR
                     ARGVACVVLKGAALRALDLYRPGERPMGDVDLLVASADLDAAAAAMADVDYVEAGTTY
                     RHRVYEPRRKAARRGFGEHVDNPLKIEIHTAVAELLPVRSVDITESLSRGAARPGLNN
                     YPDLVSLLLHLLLHAAGNMRSHALRQVQLHDIAAVANLLYENDWKDLLEGRWWLFPPL
                     ALTARYYPKQVPEDVLRAARAECPRVLRIAASRQLLTDVSWSNLRIHALPGIAWSRTP
                     LDALRYMVSRVLPSRNSLAEIEAARRDQPHLDR"
     gene            complement(1099..2478)
                     /locus_tag="VFL84_03895"
     CDS             complement(1099..2478)
                     /locus_tag="VFL84_03895"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7607794.1"
                     /translation="MADLLLTHGYFLAEDEKERQIMKPYPPLGLLYLSAFLKTRGYSV
                     DVFDSTFAERGRLVERFASERGTVGIYTTLMTRRSVLEIVRAAKRHGWRVILGGPESA
                     NYTAEYLEAGADAIVIGEGELTLAELLPALDRAGAHRLHDVPGIAFRDEEGAVVRTLE
                     RAKIKDLDSLPLPDREAIDHRLYLDAWKTHHGASSINLITARGCPYRCNWCSHAVYGY
                     THRRRSPAAVAGEMQTIVERYDPDQVWYADDVFTISHPWLAEYAAELTRRGIHKPFET
                     ITRADRLQSERSVELLRELGCYRIWIGSESGSQKILDHMERGVTVEQVRRSTQLAQAH
                     GIQVGMFLMWGYEGEEIDDIAATVEHVKRSNPDVFLTTVSYPIKGTGYFGKVRDKVAL
                     PVAWADGSDRDYVVEGRHGREYYKLADRWLRSEVEASRVAARDPARAAELMRAAGEAK
                     AAMQTWSAP"
     gene            complement(2490..3935)
                     /locus_tag="VFL84_03900"
     CDS             complement(2490..3935)
                     /locus_tag="VFL84_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7607795.1"
                     /translation="MLSILVCHSYFLRFDEKQRERAKPYPPLATLQVASLLRGAGHRV
                     AFFDAMLAAGLDDYRAKFRAARPDVVVIYEDNYNFLSKMCLRKMREACCEMIATARSG
                     GARVVAAGSDASDAPNRYLAAGADAVLLGEGLAALIAIVDRLEGDVSLPVERLVAGLP
                     DVSTASGGGATLRSSPAAPLPEPDLPKLPARDLVDVDAYRAVWREAHGYFSLNMAASR
                     GCSFRCNWCAKPIWGNRYLQRSAADVAAELLSLKRDYAADHVWFADDIFGFRVDWVRE
                     FAAILADGGGGVPFTIQLRADLASSSMAAALREAGCREVWLGAESGSQKVLDAMHKGT
                     RVGDIVEARRALGAEGIRVGFFLMLGYVGEELDDILATRDLLERTRPDDIGVSVAYPL
                     PGTKFFDLVKRELGAKRNWEESNDLDMMFVGTYRSEFYRSIRNLLHAQVAEGSSPAVR
                     RRWDELSRTEGEFRQRPEASAATRVAAHRAP"
     gene            4167..5234
                     /locus_tag="VFL84_03905"
     CDS             4167..5234
                     /locus_tag="VFL84_03905"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7607796.1"
                     /translation="MKPSHFFSRASLAAAFVATVAFPAALLAHHSTAEYDSATFVEAR
                     GEVTKVLWANPHVRLTISTQSVDGNAEPWQLEGQNPGDLDRARIPRDIVKVGDTVTFA
                     GNPSTRRDRHMYVTNVLLPDKTEIVLRANAKPRWSPEHYLSHLQATIDPARAAADRGD
                     GIFRVWLPVAANTPSWASDPPLTASARAAWLEYDAIRDDPVIDCRPPGMPQVITRSGR
                     YAIRFVRSGEDVVIENEYRDIDRVVHMTPEAAAERRAPTPLGWSTGRWDGRTLVVTTR
                     DIDFPYFQLYGLEGVPQSTEMTIVERFTPNADGTELTYDIEATDPRTFTRTVMAQGYR
                     TFRWQPGFEFMPPDCVLDAAR"
BASE COUNT          871 a         1867 c         1754 g          834 t
ORIGIN      
        1 ctcggtcgag atgcggttgg tcgcgccggg cggcctcgat ctctgccagc gaatttcggc
       61 tcggcaaaac gcggctcacc atgtagcgca gcgcatcgag cggggttcgc gaccacgcga
      121 tgccgggcag agcgtggatg cgcaagttcg accaggagac gtccgtcaac aattgacgcg
      181 aggcggcgat ccgcagaacg cgcggacact cggctcgcgc cgcgcgcaac acgtcttcgg
      241 gcacttgctt cgggtagtag cgcgccgtca acgccaacgg cgggaacaac caccagcggc
      301 cctcgagcag gtccttccaa tcgttctcgt ataagaggtt tgcgaccgcc gcgatgtcgt
      361 gcagctgcac ttgccgcagc gcgtgtgagc gcatgttgcc ggccgcatgc agcagcaggt
      421 gcagcagcag cgataccaga tcgggatagt tgttgaggcc cggccgcgcg gcgccgcggc
      481 tcaagctctc ggtgatgtcg acgctgcgca ccggcagcag ctcggcgacc gccgtgtgga
      541 tctcgatctt gagcgggttg tcgacgtgct cgccgaagcc gcgccgtgcc gccttccgcc
      601 gcggctcgta cacacggtga cgatacgtcg tgcccgcctc gacgtagtcg acgtcggcca
      661 tcgccgccgc cgcggcgtcc agatccgcgc tcgccacgag caggtcgacg tcgcccatcg
      721 gccgctcgcc ggggcggtac aggtcgagcg cccgcagcgc ggcacccttc agcacgacgc
      781 aggcgacgcc gcgcgcgcgc agcgcggtgt cgatctcgcc gagcaacgct ccgatgcgct
      841 cgtggcgaag cacactatgg ccgtgctggt ccgcgagaaa tgcctgccag gccggcgggc
      901 cgggccagac gagcttgttc ttgagcagcg tcgagatgcc ctgcatcgcc gcggcggagc
      961 gcgcgaccgc ccactcgacc tcgttccagt cgggctccga ttcgcgcggc tcggcgagct
     1021 cggatgcgag ccgctcggtc gtccggcgaa gagcggcagc caagatctcg aacgacggca
     1081 gcggcattgc ggcgccgctc atggagcgct ccacgtctgc atcgcggctt tcgcttcgcc
     1141 cgcggcgcgc atgagctcgg ccgcgcgagc ggggtcccgc gcggcgacgc gcgacgcctc
     1201 gacctcgctg cgcaaccagc ggtccgcgag cttgtagtac tcgcgcccat ggcggccttc
     1261 gacgacgtaa tcgcggtcgg agccgtcggc ccacgcgacc ggcagcgcga ccttgtcgcg
     1321 caccttgccg aagtagcccg tgcccttgat cggatacgac accgtcgtca gaaacacgtc
     1381 cgggttgctg cgcttgacgt gctcgacggt cgcggcaatg tcgtcgatct cttcgccctc
     1441 gtagccccac atcaggaaca tgccgacctg gatgccgtgc gcttgcgcga gctgcgtcga
     1501 ccgacgcact tgctcgaccg tcacgccgcg ctccatatga tcgagaattt tctggctgcc
     1561 gctctccgag ccgatccaga tgcgatagca accgagctcg cgcagcagct cgacggagcg
     1621 ctcgctttgc agccggtccg cgcgcgtgat cgtctcgaac ggcttgtgga tcccgcgccg
     1681 cgtaagctcc gccgcatatt cggcgagcca cgggtggctg atcgtgaaca cgtcgtcggc
     1741 gtaccaaacc tgatctggat cgtagcgttc gacgatcgtt tgcatctcgc cggcgaccgc
     1801 ggcggggctt cgcctgcgat gcgtgtaccc gtacacggcg tgcgagcacc agttgcagcg
     1861 gtaagggcaa ccgcgcgccg tgatcagatt gatgctgctc gcgccgtgat gcgtcttcca
     1921 cgcgtcgaga tagagccgat ggtcgatggc ttcgcgatcc ggcagcggca acgagtcgag
     1981 gtccttgatc ttcgcgcgct cgagcgtgcg cacgacggcg ccctcctcgt cgcgaaaggc
     2041 gatgcccggc acgtcgtgca atcgatgcgc gccggcgcgg tctagcgccg gcagcagctc
     2101 ggccagcgtc agctcgccct cgccgatcac gatcgcgtcc gcgcccgcct cgagatactc
     2161 ggccgtgtag ttcgcgctct cggggccgcc caagatcacg cgccagccgt gacgcttcgc
     2221 ggcgcgcacg atctcgagca ccgagcggcg cgtcatcagc gtcgtataga ttccgaccgt
     2281 gccgcgctcg ctcgcgaacc gctcgacgag ccggccgcgc tcggcgaacg tgctgtcgaa
     2341 cacgtcgacc gagtagccgc gcgtcttcag gaacgccgac aggtagagca atcccagcgg
     2401 cggatacggc ttcatgatct gccgctcctt ctcgtcctcg gcgagaaagt acccgtgcgt
     2461 gagcagcaga tcggccacgg cgcgaagcct cagggagccc gatgcgccgc gacgcgcgtc
     2521 gcggcggacg cctccggtcg ctgacgaaac tcgccttcgg ttcgtgaaag ctcgtcccat
     2581 cggcggcgca cggccggcga cgagccctcg gcgacctgag cgtgcagcag gttcctgatg
     2641 ctgcgataaa actccgagcg atacgtgccg acgaacatca tgtcgagatc gttgctctct
     2701 tcccagttgc gcttcgcgcc gagctcgcgc ttcacgagat cgaagaactt cgtgcccggc
     2761 agcgggtatg cgacgctgac gccgatgtcg tccggccgcg tccgctcgag cagatcgcgc
     2821 gtcgcgagaa tgtcgtcgag ctcctcgccg acgtagccga gcatcaagaa gaagccgacg
     2881 cggatgccct ccgccccgag cgcgcgccgc gcttccacga tgtcgccgac gcgcgtgccc
     2941 ttgtgcatcg catcgagcac tttctggctg ccgctttcgg cgccgagcca cacttcgcgg
     3001 cagcccgctt cgcgcaacgc ggcggccatg ctcgagctcg cgaggtccgc gcgcagctga
     3061 atcgtgaacg gcacgccgcc tccgccgtcg gcgaggatcg ccgcgaattc gcgaacccag
     3121 tcgacgcgaa agccgaagat gtcgtcggca aaccagacgt ggtccgccgc gtagtcgcgc
     3181 ttcaagctca agagctcggc ggcgacgtcg gcggcgctcc gctgcagata gcgattgccc
     3241 cagatcggtt tcgcgcacca gttgcagcgg aacgagcagc cgcgcgatgc cgccatgttc
     3301 aggctgaagt acccgtgcgc ttcgcgccaa acggcgcgat aggcgtcgac gtcgacgaga
     3361 tcgcgcgcgg gaagcttcgg caaatcgggc tcgggaagcg gagccgccgg gctcgagcgc
     3421 agcgtcgcac cgccgccgct cgccgtgctg acgtcgggca accctgcgac gagccgctcg
     3481 acgggcagcg acacgtcgcc ctcgagccga tccacgatcg cgatcaacgc cgcgagccct
     3541 tcgccgagca gcacggcgtc ggctccggcg gcgagatagc ggttaggcgc atccgaggca
     3601 tcggagcccg ccgcgacgac gcgcgcgccg cccgagcgcg ccgtcgcgat catctcgcag
     3661 cacgcctcgc gcatcttgcg caggcacatc ttgctcagga agttgtagtt gtcctcgtag
     3721 atgacgacga cgtccgggcg agcggcacgg aacttggctc gatagtcgtc gaggcccgcg
     3781 gccagcatcg cgtcgaagaa cgcgacgcga tgtccggccc cgcgcagcag cgaggccacc
     3841 tgcagcgtcg cgagcggcgg atagggcttc gcgcgctcgc gctgtttctc gtcgaaccgc
     3901 aggaaatacg aatggcagac caagatcgac agcaatggaa atccctcgcc ggcagcggca
     3961 acaggacttg tgtacctgcc gcgcgtcgtt ttggttctac gcgcaggaaa cggaatcgcg
     4021 cgcgccggat gaggccgtgg atccgctggt ctgtcgatgt tcttagtctt atttcgtcaa
     4081 tcagttacaa cgctaaattg aagcatgaca cgagctcgat gcgagggctg cgcttcgtcg
     4141 cgctcgcgcg taagctctga gctccgatga aaccgagcca cttcttcagc cgtgcgtctc
     4201 tcgcggccgc cttcgtcgca accgtcgcct tcccggccgc gctgctcgcc caccattcga
     4261 cggccgagta cgactccgcc acgttcgtcg aggcgcgcgg cgaggtcacg aaggtgctgt
     4321 gggcgaatcc gcacgtgcgc ctgacgatca gcacgcagag cgtcgacggc aacgccgagc
     4381 cgtggcagct cgagggccag aaccccggcg acctcgatcg cgctcggatt ccgcgcgaca
     4441 tcgtcaaggt cggcgatacg gtcacgttcg cgggcaaccc gtcgacacgc cgcgaccggc
     4501 acatgtacgt cacgaacgtc ttgctccccg acaaaaccga aatcgtgctg cgcgcgaacg
     4561 cgaagccgcg gtggtcgccg gagcattact tgagccacct gcaggcgacg atcgatcccg
     4621 cacgcgccgc cgcggaccgc ggcgacggca tctttcgcgt gtggctgccg gtcgccgcga
     4681 acacgccgag ctgggccagc gatccgccgt tgacggcgag cgcgcgcgcg gcctggctcg
     4741 agtacgacgc gatccgcgac gaccccgtca tcgactgccg cccgcccggc atgccgcagg
     4801 tcatcacgcg cagcggccgg tacgcgattc gcttcgtgcg ctcaggcgag gacgtcgtca
     4861 tcgagaacga gtaccgcgac atcgatcgcg tcgtccacat gacgccggag gcggccgcgg
     4921 aacggcgcgc gccgacgccg ctcggctggt cgacgggccg ctgggacggc cggacgctcg
     4981 tcgtgacgac gcgcgacatc gacttcccct acttccagct ctacggcctc gagggcgtgc
     5041 cgcagagcac cgagatgacg atcgtcgagc gcttcacgcc gaacgcggac ggcacggagc
     5101 tcacctacga catcgaagcg accgatccgc gcacgttcac gcgcacggtc atggcgcagg
     5161 gctaccgcac gttccgctgg cagcccggtt tcgagttcat gccgcccgac tgcgtcctcg
     5221 acgcggcacg ctgaacggag aagtgccggt gttacggttc ggtttaaccg aaccgcaaaa
     5281 cggagaggtg ccggtgttac ggttcggttc aaccgaaccg taacac
//
LOCUS       DASSNN010000212         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1051259, whole genome shotgun sequence.
ACCESSION   DASSNN010000212 DASSNN010000000
VERSION     DASSNN010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051259"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..994)
                     /locus_tag="VFL84_03910"
     CDS             complement(158..994)
                     /locus_tag="VFL84_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7607797.1"
                     /translation="MKAYSTSEVAELLGLSTARVRSLIRSGVVTPQKGDGGRASFTFQ
                     DLVLLRTAKGLIDANVPARRITRALQALASQLPTDRPLSAVRVQIDGDRVIVRDNASS
                     WEPESKQTVLDFSVKELGEKIAPLARDSVQRALRAASTADDLFHAALESEEIGATADA
                     ESAYKNAIAADPKHVSAHINLGRLRHAAKALDEAERLYRRALELEPSHPTARFNLGVV
                     LEDRGATKEAIAEYVEAARLDPRVADVHFNLARLYQQTGDQQAALRHFSRFRALTRDR
                     ID"
     gene            complement(1001..1855)
                     /locus_tag="VFL84_03915"
     CDS             complement(1001..1855)
                     /locus_tag="VFL84_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET7607798.1"
                     /translation="MPARALSTATISFGLVSIPVKLYSAAESKAALSFNQIHKKDGSR
                     VKQQLISSRTGEVVPKDEIVKGYEFAKDQYVLFEPDELKSLEAVATHTIDITEFLKAE
                     QIDRRYLDKVYYLGTEKGGARAYKLLAQVLVDTGRVAIGKYAARGKQYLVMVRPMENG
                     LVMEQLHYPDELRAFGEVPIEDAEVKPAELKLATQLVEQAASDEFKPESYKDEVRERM
                     LALIQRKVEGEDITTAPTAEPEHKIIDIMEALKASLAAGKKPAQAAADDKKAEAKPKA
                     AAKKRAAK"
     gene            1968..>2727
                     /locus_tag="VFL84_03920"
     CDS             1968..>2727
                     /locus_tag="VFL84_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HET7607799.1"
                     /translation="MPGGQRFVVQQHAARRTHYDFRLEFDGVLKSWAVPKGPSPNPAD
                     KRLAVHVEDHPVDYVNFEGVIPPDNYGAGAVIVWDRGQWLALNDIAAGFDKGKLLFEL
                     RGHKLRGKWTLVKTKRGKNEWLLIKERDAYATTQGTEDYPHDSVMSGRTVEQVAGGDP
                     RGAEVVAKLRKLGAVQRELKARDLRPMLATPGKPFSDPKWVFELKYDGYRLFGEKRAG
                     EVRLYSRAGNDFTATFPEIAEIVVALPFPQLIIDA"
BASE COUNT          442 a          884 c          895 g          506 t
ORIGIN      
        1 aaactggcgg tgcactcctg gacgaaagaa agcgcagact actggtcgtt taacccaaag
       61 ctgcctagaa agcgcggccc atccggcctc ggcggtccac ccgagcgttc caagcaatga
      121 atgcgggaga cgctagacga gcgctccttc gaacccgtca gtcgattcga tcgcgcgtca
      181 atgcgcggaa gcgcgagaag tgccgcagcg cggcctgctg atcgccggtc tgttgataaa
      241 gacgcgcgag attgaagtgc acgtccgcga cgcgcggatc gaggcgcgcc gcctcgacat
      301 actcggcgat tgcctctttc gtcgcgccgc ggtcctctag cacgacgccc aagttgaacc
      361 gcgccgtcgg atggctcggc tcgagctcga gcgcgcgccg gtagagccgc tccgcttcgt
      421 cgagcgcctt cgccgcgtgt ctcaaccggc cgaggttgat gtgcgcggag acgtgcttcg
      481 gatcggcggc gattgcgttc ttgtaagcgc tctccgcgtc ggccgtcgcg ccgatctcct
      541 cgctttcgag cgcggcatgg aataggtcgt cggccgtcga cgccgcgcgc agtgcgcgct
      601 gcaccgagtc gcgcgcgagc ggcgcgattt tctcgccgag ctccttgacg gaaaagtcga
      661 gcaccgtttg cttcgactcc ggctcccagc tgctcgcgtt gtcgcgaacg atcacgcggt
      721 cgccgtcgat ctgcacgcgc acggccgaca gcggccgatc cgtcggcagc tggctcgcga
      781 gcgcttgcag cgcccgcgtg atgcgccgcg cgggcacgtt cgcgtcgata agccctttcg
      841 cggtgcgaag cagcacgagg tcttgaaacg tgaacgacgc gcggcccccg tcgcctttct
      901 gcggcgtcac gacgccggag cggatcagcg agcggacgcg cgcagtcgac agaccgagaa
      961 gctcggcgac ttcgctcgtg gaataggctt tcatgcgcgc ctacttggcg gcgcgcttct
     1021 ttgcggcggc tttcggcttc gcctccgcct tcttgtcgtc cgccgcggct tgcgccggct
     1081 tcttgccggc cgcgaggctc gccttcagcg cttccatgat gtcgatgatt ttgtgctccg
     1141 gctcggctgt cggcgcggtc gtgatgtctt cgccttcgac cttgcgctgg atcaacgcca
     1201 acatgcgctc gcgcacttcg tccttgtagc tctcgggctt gaactcgtcg ctggccgctt
     1261 gctcgacgag ctgtgtcgcg agcttgagct cggcgggctt cacctcggcg tcctcgatcg
     1321 gcacctcgcc gaacgcgcgc agctcgtcgg gatagtggag ctgctccatg acgaggccgt
     1381 tctccatcgg ccgcaccatg acgagatact gcttgccgcg cgcggcgtat ttgccgatcg
     1441 cgacacggcc cgtgtcgacg agcacttgcg cgagcagctt gtatgcgcgc gcgccgcctt
     1501 tctcggtgcc caagtagtag accttgtcga gatagcggcg gtcgatctgc tcggccttca
     1561 agaattcggt gatgtcgatc gtgtgcgtgg ccacggcctc gagcgacttg agctcgtcgg
     1621 gctcgaacag cacgtactgg tccttcgcga actcgtagcc cttgacgatc tcgtccttcg
     1681 ggacgacctc gcccgtgcgc gaagagatca gctgctgctt gacgcgcgaa ccgtccttct
     1741 tgtggatctg gttgaaggac aaagctgctt tcgactcggc ggccgaatac agcttcaccg
     1801 ggatcgacac gagaccaaac gatatggtgg cggtcgagag tgctcgcgct ggcatgggcg
     1861 gatactatat cctcgctggc gcggtgacaa caaaaaatgt cggcaactta gagagttatc
     1921 gcgcaaagcg aaaggccgat cgaactccgg agcccttcgg cgctgcgttg ccgggggggc
     1981 agcggttcgt cgtgcagcag cacgcggcgc ggcggactca ttacgacttt cggctcgaat
     2041 tcgacggcgt gctgaaatcc tgggccgttc ctaaggggcc gtcgccgaat cctgccgaca
     2101 agcgtctcgc cgtccatgtc gaggatcacc ccgtcgacta cgtcaacttc gagggcgtga
     2161 tcccgcccga taactacggc gcgggcgccg tcatcgtctg ggaccgcggc cagtggctcg
     2221 cgctgaacga catcgccgcg gggttcgaca agggcaagct gctgttcgag ctgcgcggcc
     2281 acaagctgcg cggcaagtgg acgctcgtca agacgaagcg cggcaagaac gagtggctgc
     2341 tcatcaaaga gcgcgacgcg tacgcgacga cgcaaggtac cgaggattat ccgcacgact
     2401 ccgtgatgtc ggggagaacc gtcgagcagg tcgccggcgg cgatccgcgc ggcgcggaag
     2461 tggtcgccaa gctgcgcaag ctcggcgccg tgcagcgcga gctgaaagcg cgcgacctgc
     2521 ggccgatgct cgcgacgccg ggcaagccgt tctcggaccc gaagtgggtg ttcgagctca
     2581 agtacgacgg ttaccgcttg ttcggcgaga agcgcgccgg cgaagtgagg ctctactcgc
     2641 gcgcgggcaa cgacttcacg gcgacgttcc ccgagatcgc cgagatcgta gtggccttgc
     2701 cgtttccaca gctcatcatc gatgccg
//
LOCUS       DASSNN010000213         2857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_421643, whole genome shotgun sequence.
ACCESSION   DASSNN010000213 DASSNN010000000
VERSION     DASSNN010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421643"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="VFL84_03925"
     CDS             complement(<1..1237)
                     /locus_tag="VFL84_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HET7607800.1"
                     /translation="MGLARTLSRGQAGLDAFLVTVEVHVAGGLPGFTVTGLPAAAVRE
                     SKDRVRAALTNSGHEVPASRITVHLGPADIPKTGGRFDLAIALGVVLAQQPATGSADG
                     IEFLGELALDGELRPVNGALPAVLAARESGHPLVLPAANAAEAALVADAEVYAAAHLK
                     EVVSHLRGERRLPRVAARTEPLTTDATLDIGDVRGQTFAKRALVVAAAGGHNLLMIGP
                     PGSGKSMLAERLSGLLPPLTFDEMLRVASIASVAGEPRAAEPSLSPPFRAPHHTTSAQ
                     ALVGGGTRPRPGEISLAHRGVLFLDELPEFARGALEALREPLESGVARISRVHEQLTF
                     PAEFTLVAAMNPCYCGFRGDGTDRCKCTPYKLEQYRSRISGPLLDRFDLHVEVPRVSF
                     ADIAEPAEKAESALLREDVV"
     gene            complement(1271..1537)
                     /locus_tag="VFL84_03930"
     CDS             complement(1271..1537)
                     /locus_tag="VFL84_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HET7607801.1"
                     /translation="MDDKKSTRFSLDELARQLAASVPQNLKVLGQDLERNFKTLLQSG
                     IERMELVSREEFDVQRAVLERTRSKLEAMEGRLAELEKALASRK"
     gene            1742..2200
                     /locus_tag="VFL84_03935"
     CDS             1742..2200
                     /locus_tag="VFL84_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET7607802.1"
                     /translation="MNLRVRHLTLLLALLGTAAYAADVYKSTAPDGTVIYSDRPEGAD
                     AQFIYSATPSAPRTQPGVAQAGRAASGAQAASPPQTPAIPTLSAGPTSAQLKAERQKN
                     CEIAKETQQRYTLSRRLFRTNAVGEREYLDDAAVAEARAKAAADVKDWCD"
     gene            2178..>2857
                     /locus_tag="VFL84_03940"
     CDS             2178..>2857
                     /locus_tag="VFL84_03940"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7607803.1"
                     /translation="MSKTGAIDRPPEDRTPRTTLIPPPRASASAETVTTPAFDSNADA
                     FYIPDFCATRMVFAVVLIAELVAVTLALARPDAAFLTELARISLFLQWLGLTNAALLC
                     YSRRWLARLTVAQSSVAVFALILLNTIVISELSLMLGTAFGAVGITETPTDHWPFLLR
                     NAGIAVIVTALLLRYFFVTHQWQKHVRAEALSRIQALQARIRPHFLFNSMNTIAALTR
                     SDPKRAEEA"
BASE COUNT          439 a         1006 c          975 g          437 t
ORIGIN      
        1 cgacgacgtc ctcgcgcagc agcgccgact cggctttttc ggcgggctcg gctatgtccg
       61 caaacgagac gcgcggcacc tcgacgtgca gatcgaatcg gtcgagcagc ggacccgaga
      121 tgcggctgcg atattgctcg agcttgtatg gcgtgcactt gcagcgatcg gtgccgtcgc
      181 cgcggaatcc gcaatagcac ggattcattg cggcgacgag cgtgaactcg gccgggaacg
      241 tgagctgctc gtgcacgcgc gagatccgcg cgacgccgct ctcgagcggc tcgcgcagcg
      301 cctcgagcgc gccgcgcgcg aactcgggca gctcgtccaa gaacagcacg ccgcgatgcg
      361 cgagcgagat ctcgccgggc cgcggccgcg tgccgccgcc gacgagcgct tgcgccgacg
      421 tcgtgtgatg cggcgcgcga aacggcggcg acagcgacgg ctcggccgcg cgtggctcgc
      481 cggcgaccga cgcgatgctc gcgacgcgca gcatctcgtc gaaagtcagc ggcggcagca
      541 ggccgctcaa tcgctcggcc agcatgctct tgccgctgcc gggagggccg atcatgagca
      601 ggttgtgccc gcccgctgcc gcgacgacga gcgcgcgctt cgcgaacgtc tgaccgcgca
      661 cgtcgccgat gtcgagcgtc gcgtccgtcg tcagcggctc cgtgcgcgcg gcgacacgcg
      721 gcagccgccg ctcgccgcgc aggtggctca cgacttcttt caggtgcgcg gccgcataga
      781 cctcggcgtc ggcgacgagc gcggcctccg ccgcgttcgc ggcgggcagc acgagcggat
      841 ggccgctctc gcgcgcggcg agcacggccg gcagcgcgcc gttgaccggg cgcagctcac
      901 cgtcgagcgc gagctcgccg aggaactcga tgccgtcggc ggacccagtc gcaggttgct
      961 gcgcgagcac gacgccgagc gcgatcgcga ggtcgaagcg gccgcccgtt ttcggaatgt
     1021 cggcgggccc aaggtgcacc gtgatgcggc tcgccggaac ctcgtggccc gagttcgtca
     1081 gcgcggcgcg cacgcggtcc ttgctctcgc ggacggccgc cgccggcaag cccgtgaccg
     1141 tgaacccggg caacccgccc gcgacgtgga cctcgacggt gacgagaaac gcatcgagcc
     1201 cggcctgccc gcggctcaga gtgcgcgcga gccccacggt caggggctgg ccggcagggc
     1261 cggccggcgt ctatttgcgc gacgccaaag ccttctcgag ctcggcgagc cggccttcca
     1321 tggcctcgag cttcgagcgc gtgcgctcga gcacggcacg ctggacgtcg aactcctcac
     1381 ggctcacgag ctccatgcgc tcgatgccgg actgcagcag cgtcttgaag ttgcgctcca
     1441 ggtcctggcc gagcaccttg aggttctgcg ggaccgaggc ggccagctgg cgggcgagct
     1501 cgtccaggct gaaacgcgtc gatttcttgt cgtccattgc atgctctccc gtagccgaag
     1561 ggccagcgcc gaatatgggg atttgggcct gaaaggcatc atagccggcg ccctaggcgc
     1621 ggtccgcaat ttcacttaat gtgtaggcgc gaaacccgtg aaagcggcgt gaattgaatc
     1681 acgggtcgcg cgggcgcggc ttgttagact cgccgagcga atccgcctga ggcccacgac
     1741 catgaacctt cgagtcaggc atctcacctt attgctggcg ttgctcggca cggcggccta
     1801 tgcggccgac gtctacaagt cgacggcgcc cgacggcacc gtcatctaca gcgaccggcc
     1861 agagggcgcg gatgcgcaat tcatctactc cgccacgccg agcgcgccgc gcacgcagcc
     1921 gggtgtggcg caggctggaa gggccgcgag cggcgctcag gccgcttcgc ctccgcagac
     1981 gccggctatc ccgacgctgt cagccggtcc gacgtccgcg cagctgaagg ccgagcggca
     2041 gaagaactgc gagatcgcaa aagagacgca gcagcgctat acgctgtcgc gccggctgtt
     2101 ccgcacgaac gccgtcggcg agcgtgagta tctcgacgac gcggcggtcg ccgaagcccg
     2161 tgccaaggcg gcggccgatg tcaaagactg gtgcgattga tcgaccgccc gaagatcgca
     2221 ccccgcgcac gacgttgata ccgccgccgc gcgcgagcgc gtcggccgag accgtcacga
     2281 cgcccgcctt cgattcgaac gccgacgcgt tctacatccc tgacttctgc gcgacgcgca
     2341 tggtgttcgc ggtcgttctg atcgcggagc tcgtcgccgt cacgctcgcg ctcgcgcggc
     2401 ccgatgcggc gtttctgacg gagctcgcgc ggatctcgct gttcctgcag tggctcgggc
     2461 tcacgaacgc cgcgctgctc tgctattcgc gccgctggct cgcgcgcctc acggtggcgc
     2521 aaagctccgt cgccgtattc gcgctgattc tgctcaacac gatcgtgatc tcggagcttt
     2581 cgctgatgct cggcacggct ttcggcgccg tcggcatcac ggagacgccg accgaccatt
     2641 ggccgttcct gctgcgcaac gccggcatcg cggtgatcgt gacggcgctg ctgctgcgct
     2701 acttcttcgt cacgcatcaa tggcagaagc acgtgcgcgc cgaagccctg tcgcgcatcc
     2761 aagcgctgca agcgcgcatt cggccgcact ttcttttcaa cagcatgaac acgatcgcgg
     2821 cgctgacccg cagcgacccg aaacgcgctg aggaagc
//
LOCUS       DASSNN010000214         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_171523, whole genome shotgun sequence.
ACCESSION   DASSNN010000214 DASSNN010000000
VERSION     DASSNN010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171523"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1843
                     /locus_tag="VFL84_03945"
     CDS             68..1843
                     /locus_tag="VFL84_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET7607804.1"
                     /translation="MSAIEKPNVPHLDPVTLEVIRNALPAISDEMSVDLQRTSYNMMI
                     YEVRDYCTALLDVDGSLISQNIGGVSHFVADLGVVVKDAVARYGRDGFAPGDVLLHNH
                     QATAGQHLNNVVVYSPVFYRGELVAFAMVRAHWVDVGGLSTGFGAGNRAYDPWSEGLQ
                     FNQLKIYEAGVVDKKLLAFIRDNIRFPDNAMGDMRSQLAACRLGERRFVELLDRYGLG
                     IVQASIRQFYLETERKCRAAVAAIPDGVYEAESYIDWRGKGYDIKVKVIVDGSDMTID
                     LSGCSPEREGGANSRTYAGAYIAYKALTTPTEPLNEGAFSALKVIIPEGNMMMARFPA
                     FMAGWSMPLPTVVDTILLALARAVPDQIPAAHSGSLGAALAYSGRDRKRNRDFVLMSI
                     ESGGWGGRGDADGEDVSMSVCQGDVRNAPIENLELKGPVIVEERALRPDSGGPGKFRG
                     GLGVRTRIRSLEGGRINVQGGDGGRLTCPPWGLVGGGPGAVATTLVNAPGDAELHVPK
                     SQPLTWPAGTEVLYMTAGGGGWGDPLERDPERVLRDVREEYISETHAKEQYGVVLTAG
                     RASVDAAATAALRRELRERRSRGAK"
     gene            1840..>2154
                     /locus_tag="VFL84_03950"
     CDS             1840..>2154
                     /locus_tag="VFL84_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607805.1"
                     /translation="MTANLAPADAAVRARSVARRHQVLRAWLAANDAEALVAYGSGHH
                     AFTGTNPAWYVAAYKQLGPHMAVILPLAGEPLLVLTPTWDAARYRERATMEFVAVQPD
                     DFL"
BASE COUNT          339 a          739 c          763 g          313 t
ORIGIN      
        1 ttcccggcga tcgcctcacg gtcgccgaga cgggcgaatt gctgatcacg gtgcggcgcg
       61 gcgaagcatg agcgcgattg agaagcccaa cgtcccgcac ctcgaccctg tcacgctcga
      121 ggtcatccgc aacgcgctgc ccgcgatctc ggacgagatg agcgtcgact tacagcgcac
      181 gtcgtacaac atgatgatct acgaggtgcg cgactactgc acggcgctgc tcgacgtcga
      241 cggctcgctg atctcgcaga acatcggcgg cgtctcgcac ttcgtcgcgg acttgggcgt
      301 cgtcgtcaag gacgccgtcg cgcgctacgg ccgcgacggc ttcgcgcccg gcgacgtgct
      361 cttgcacaac catcaggcga cggccggcca gcacttgaac aacgtcgtcg tctactcgcc
      421 ggtgttctat cgcggcgagc tcgtcgcgtt cgcgatggtg cgcgcgcact gggtcgacgt
      481 cggcgggctc tcgaccggct tcggcgcggg aaaccgcgcg tacgacccgt ggagcgaagg
      541 cctccagttc aatcagctga aaatctacga agccggcgtc gtcgacaaga agctgctcgc
      601 gttcattcgc gacaacatcc gcttccccga caacgcgatg ggcgacatgc gctcgcagct
      661 cgcggcgtgc cggctcggcg agcggcgctt cgtcgagctg ctcgaccgct acggcttagg
      721 catagtgcag gcttcgatcc gccagttcta tttggagacg gagcggaagt gccgcgcggc
      781 cgtcgccgcg attccggacg gcgtctacga agccgagagc tacatcgact ggcgcggtaa
      841 aggctacgac atcaaggtca aagtcatcgt cgacggcagc gacatgacga tcgacctctc
      901 gggctgctcg ccggagcgcg aaggcggcgc gaactcgcgc acctacgcgg gcgcctacat
      961 cgcgtacaaa gcgttgacga cgccgaccga gccgctcaac gaaggcgcgt tctcggcgct
     1021 gaaggtcatc atccccgaag gcaacatgat gatggcgcgg tttcccgcgt tcatggcggg
     1081 ctggagcatg ccgctgccga ccgtcgtcga cacgatcctg ctcgcgctcg cgagagccgt
     1141 gccggaccaa attccggccg cgcacagcgg ctcgctcggc gcggcgctcg cgtactcggg
     1201 ccgcgatcgc aagcgcaacc gcgacttcgt gctcatgagc atcgagtcgg gcggctgggg
     1261 cgggcgcggc gatgccgacg gcgaggacgt ctcgatgtcc gtctgccaag gcgacgtgcg
     1321 caacgcgccg atcgagaacc tcgagctcaa gggccccgtc atcgtcgagg agcgcgcgct
     1381 gcgcccggac tcgggcggcc ccggcaaatt ccgcggcggg ctcggcgtgc gcacgcggat
     1441 ccgctcgctc gagggcggcc gcatcaacgt gcaaggcggc gacggcgggc ggctcacgtg
     1501 cccgccgtgg gggctcgtcg gcggcgggcc gggcgccgtc gcgacgacgc tcgtcaacgc
     1561 gcccggcgac gccgagctgc acgtgccgaa gagccagccg ctgacgtggc ccgcgggcac
     1621 ggaggtgctg tacatgacgg cgggcggcgg aggttggggc gacccgctcg agcgagaccc
     1681 cgagcgcgtg ctgcgcgacg tgcgcgagga gtacatctcc gagacgcacg cgaaagagca
     1741 gtacggcgtc gtgttgacgg cgggccgtgc gtcggtcgat gcggcggcga ccgccgcgct
     1801 gcgccgcgag ctgcgcgagc gccgatcgcg aggcgcgaaa tgacggcgaa tctcgcgccg
     1861 gccgatgcgg ccgtgcgcgc gcgcagcgtc gcgcggcgtc atcaagtgct gcgcgcgtgg
     1921 ctcgcggcca acgacgccga ggcgctcgtc gcgtacggct cgggtcacca cgcgttcacg
     1981 ggcacgaacc ccgcgtggta cgtggccgcg tacaagcagc tcggcccgca catggccgtg
     2041 atcttgccgc tcgccggcga gccgctgctg gtgctgacgc cgacctggga cgccgcgcgc
     2101 taccgcgagc gcgcgacgat ggaattcgtc gccgtgcagc ccgacgattt tctg
//
LOCUS       DASSNN010000215         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1051927, whole genome shotgun sequence.
ACCESSION   DASSNN010000215 DASSNN010000000
VERSION     DASSNN010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051927"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFL84_03955"
     CDS             complement(<1..680)
                     /locus_tag="VFL84_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7607806.1"
                     /translation="MKLWACIGLGIIALAPAAYAADTNLTIEVPDVALAIPRVSPPHF
                     QQEGLPYLTENQLIVDLLTLYGKADYEAALARAKEDLGPELALLETGDPVRLLAGRVG
                     PGRLPIPPTAGEDVSASVLYVIGVIYFELERYAPAEAALKAALAPLPDYLRVHEALGM
                     LYLRTERYDEARMHLARAAELGLNTAGLHISLAYINAKSENWWGAASAFEQALAIEPE
                     NRAWQTGL"
     gene            complement(677..1414)
                     /locus_tag="VFL84_03960"
     CDS             complement(677..1414)
                     /locus_tag="VFL84_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607807.1"
                     /translation="MMTGRKRRWLAASLATGLVLLLVFIASFDMRGNRQLPERFVLTE
                     VTTLEAPPPPPPPAPRPSGDLRSGGSTGMDLTLRRSTAPAPLDTMKLDVQFAAAEVGP
                     LNIAGLGSGIGLGIGDGIGNGRGTGFGLATISELDQIPTVVSAPVFPFPDEARARGLT
                     EFDLRYHILIDEEGRVYPISLVENPFPTLAGEFLDFASRVTFTAPTRLGIPVRTEYMW
                     PVKIRMNPSVQTPAPYVPSMRRVVPAR"
     gene            complement(1411..1818)
                     /locus_tag="VFL84_03965"
     CDS             complement(1411..1818)
                     /locus_tag="VFL84_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7607808.1"
                     /translation="MRPSLQERLDSDRPPGIDLAPMLDTVFNLLIFFIIASAFVQESG
                     IDIEKPQSVTATRLKNDFIVLSISPTGEVWYDRNNIGVEGVRSTVAALLLAEERPVVI
                     EADKRATTDVLVRVMDEAKLAGAKTLNIATAVQ"
     gene            complement(1822..2205)
                     /locus_tag="VFL84_03970"
     CDS             complement(1822..2205)
                     /locus_tag="VFL84_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7607809.1"
                     /translation="MIDRFVLFAFADNFVVWGIVALALFCYVVEIDLILAERDAHWKR
                     QAGTWLTVLPILISALPLLGLLGTVNGLLTTFRTMAGTVVVNQLDFVSTGVADAMVTT
                     QLGMITAIPGLLMYTYLRGRRGALP"
     gene            complement(2202..>2898)
                     /locus_tag="VFL84_03975"
     CDS             complement(2202..>2898)
                     /locus_tag="VFL84_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7607810.1"
                     /translation="GIEELHRGSTGVVTFDPTLTRALLLAETQEGVWQHLRRGGFWAI
                     PIVLFGLFASTIAVLKAVSLYRLPIPVPALAERVQSALARGGDAAQTVAAQIQGPQGE
                     LVRIAIGAESQEARDDRLHGTLLQQRIRLERWLSAIALTASVSPLLGLLGTVSGMIAT
                     FQAMSLFGAGDASAVSGGVGEALINTELGLVVAIPALLAHALLSRKAKSYLAQLESDA
                     VNLSRLSPPSGAA"
BASE COUNT          498 a          956 c          984 g          460 t
ORIGIN      
        1 aacagcccgg tttgccaggc tcgattctcg ggctcgatcg caagcgcctg ctcgaacgcg
       61 cttgccgctc cccaccagtt ctcgctcttg gcgttgatgt acgccaacga tatgtggagg
      121 ccggccgtat tgaggccgag ctcggccgcg cgcgcaagat gcatgcgcgc ttcgtcgtag
      181 cgctcggtcc ggaggtacag catgccgagc gcctcgtgga cgcgaagata gtcggggagc
      241 ggtgcgagcg cagccttgag cgccgcctcc gcgggtgcgt agcgctcgag ctcgaaatag
      301 atcacgccga tcacgtagag caccgacgcg ctgacgtcct cgcccgcggt aggaggtatc
      361 ggcaaccgtc ccggtcccac gcgccccgcg agcaggcgga ccgggtcgcc ggtctcgagc
      421 agcgcgagct cgggcccgag gtcctccttc gcgcgcgcca acgcggcttc gtagtccgct
      481 ttgccgtaga gcgtcagcag gtcgacgatg agctgattct cggtcaggta aggcaggcct
      541 tcctgctgga agtgcggggg gcttacgcgc gggatcgcga gcgcaacgtc gggcacctcg
      601 atcgtcaggt tcgtgtcggc ggcgtacgcc gccggcgcga gcgcgatgat accgagccct
      661 atgcacgccc agagcttcat cgagctggca ccacgcgccg catcgaaggc acgtacggcg
      721 ctggcgtctg cactgacggg ttcatcctga tcttgacggg ccacatgtac tcggttcgca
      781 ccggaatgcc gaggcgcgtg ggagcagtga aggttaccct cgaggcgaag tccaagaact
      841 cgcccgccag cgtcgggaac gggttctcga cgagcgaaat cgggtacacg cggccttcct
      901 cgtcgatcag gatgtggtac ctgagatcga actccgtcaa cccgcgcgcc ctggcctcgt
      961 cgggaaacgg gaatacgggt gccgagacga cggttggtat ttggtccagc tcggaaatcg
     1021 tcgcgagccc gaagccggtc ccgcgtccgt tgcctatgcc gtcgccgatg ccgagcccga
     1081 ttcccgaccc gagccccgcg atgttcagtg ggccgacctc ggcggccgcg aactgcacgt
     1141 cgagcttcat ggtgtcgagt ggagcgggag ccgtgctgcg ccgcagagtc aaatccatgc
     1201 ccgtggagcc gccactgcgc aaatcgcccg atggacgggg cgcgggcgga ggtgggggcg
     1261 ggggcgcctc caaggtcgtg acttcggtca agacgaagcg ctcgggcagc tgccggttcc
     1321 cgcgcatatc gaaggatgcg atgaagacga gcagcagcac gagcccggtt gccagcgaag
     1381 cggcgagcca gcggcgtttg cgccccgtca tcattggacg gcagtcgcga tgttcagtgt
     1441 cttcgcgccg gcgagcttcg cttcgtccat gacgcgcacg agcacgtcgg tcgtggctct
     1501 tttgtcggcc tcgatcacga cgggccgctc ttcggcgagc agcagcgccg ccaccgtcga
     1561 gcgcacgcct tcgacgccga tgttgttgcg gtcgtaccag acttcgcccg tcggactgat
     1621 cgagagcacg atgaagtcgt tcttgagccg cgtcgctgta acggactgcg gcttctcgat
     1681 gtcgatgccc gactcctgga cgaaagcgct cgcaatgatg aagaagatga gcaggttgaa
     1741 aacggtgtcg agcatcggcg cgaggtcgat gcccggcggg cggtcgctat ccaagcgctc
     1801 ttgcagcgag ggtctcatgg ctcagggcag cgcgccccga cggccgcgca agtacgtgta
     1861 catcagcagt cccggaatcg ccgtgatcat tccgagctgc gtcgtgacca tcgcgtccgc
     1921 gacccccgtg ctgacgaagt cgagctgatt tacgacgacg gtgcccgcca tcgtgcggaa
     1981 cgtcgtgagc aggccgttga ccgtgccgag cagcccgagc agcggcagcg cgctgatcag
     2041 gatcggcagc actgtgagcc acgtgccggc ctggcgtttc cagtgcgcgt cgcgctcggc
     2101 gaggatcagg tcgatctcga cgacatagca gaagagcgcc agagcgacga tgccccagac
     2161 gacgaagttg tccgcgaacg cgaacagcac gaatcgatcg atcatgccgc acccgacggc
     2221 ggcgacagcc ggctcaagtt gacggcatcg ctctcgagct gcgcgaggta gctcttcgcc
     2281 ttgcgcgaca gcaacgcgtg cgcgagcaat gccggaatcg cgacgacgag gccgagctcg
     2341 gtgttgatca gcgcctcgcc gacgccgccc gagacggcgc tcgcgtcgcc ggcgccgaac
     2401 agcgacatcg cttggaacgt cgcgatcatg ccggagaccg tgccgagaag cccgagcagc
     2461 ggcgacacgg aggccgtcag cgcgatcgcg ctcaaccagc gttcgagccg aatgcgttgc
     2521 tgcagcaacg tgccgtgcag ccgatcgtcg cgcgcttcct gcgactccgc tccgattgcg
     2581 atgcggacca gctcgccttg cggaccttga atttgcgcgg caaccgtctg cgcggcgtcg
     2641 ccgccgcgcg cgagcgcgga ctgcacgcgc tcggcgagcg ccggcacagg tatcggcaga
     2701 cggtacagcg agacggcctt cagaaccgcg atcgtcgacg cgaacaaccc gaacagcacg
     2761 atcgggatgg cccagaatcc gccgcggcgc aagtgctgcc aaaccccttc ctgcgtctcg
     2821 gcgagcagca gtgcccgcgt cagcgtcggg tcgaacgtga cgacgccggt cgagccgcga
     2881 tgcagctcct cgatgccc
//
LOCUS       DASSNN010000216         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1052170, whole genome shotgun sequence.
ACCESSION   DASSNN010000216 DASSNN010000000
VERSION     DASSNN010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052170"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..603
                     /gene="hcrC"
                     /locus_tag="VFL84_03980"
     CDS             133..603
                     /gene="hcrC"
                     /locus_tag="VFL84_03980"
                     /EC_number="1.1.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit gamma"
                     /protein_id="HET7607811.1"
                     /translation="MSKRLLKVTVNGRAREDAVHDHALLVDYLRGIGQTGVKQGCDGG
                     ECGACTVLVDGEPRLSCITLAASVEGRAVETIESLAQNGRMSRLQAAFHEKLGTQCGF
                     CTPGMIMSADALLRRNANPSEDEIRKALGGNLCRCTGYSKIVESVQCACAGREG"
     gene            636..>2423
                     /locus_tag="VFL84_03985"
     CDS             636..>2423
                     /locus_tag="VFL84_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008387060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7607812.1"
                     /translation="MLREGSIGCPTPLIDGVEKVTGRARYTADLPATDALAGAILRSP
                     VAHGVIRGIDTSRARALPGVRAVVTGQDFVAPYGVIPIAQNEWPLARDRVRYRGEPVA
                     AVAAVDRATAQAALAAIVLDIEPLPAYFAAADARAEGAVLLHDRKPGNVEREVDHVFG
                     DVDAGFAAADLVREQTFHYAEVAHGQIERNGALATYEPEHARLTMQSTTQVPYYLHLT
                     LAQCLGLDGAQVRVIKPFVGGGFGHRVEPLNFEMVAGALARAAGGVVRVEQSREDSFL
                     THRGRPETDIRLKLGVKRTGEITAVDCEIVQRGGAYAGYGLVTILYAGALLHALYKVG
                     AVRYRGYRIYTNTPPCGAMRGHGAVDARHAFEAMLDAMASELGLDPIALRRANLIEPP
                     YRTLNDLQVNSYGIPECLDWVERASGWRERHGKLPRGRGLGLACSHYVSGSAKPVHWS
                     GEPHAVVNLRLDFDGGITLLTGAADIGQGSSTLLAQVAAEVLLLPLSRIRVIAADSAL
                     TPKDNGSYSSRVSFMVGNAAMRAAQALLDVLVAAAARKLDVAPAEIEWLGEACRVRGG
                     ERALDFAAVVTAALADNGPVTVKGTWSAPP"
BASE COUNT          373 a          831 c          862 g          357 t
ORIGIN      
        1 cgcaaggcaa ggcggtgcgc gtgcgcgacc tccgcccgaa atgatccccc cacgctcgct
       61 ccgctcgctg ccccccgggg ggcggatcaa tcgctcgggg cggcccttcg cgattgaagg
      121 agaaaaatag agatgagcaa acgcttgctg aaagtaacgg tcaacgggcg tgcgcgcgag
      181 gatgcggtcc acgatcacgc actgctcgtc gattacctgc gtggcatcgg ccagaccggc
      241 gtcaagcagg gctgcgatgg cggcgaatgc ggcgcgtgca cggtgctcgt cgacggcgag
      301 ccacgacttt cctgcatcac gctcgccgcc tcggtcgaag gtcgtgccgt cgagacgatc
      361 gagtcgctcg cgcagaacgg ccggatgagc cgcctgcagg ccgcgttcca cgaaaagctc
      421 ggcacgcaat gcggcttctg cacgccgggg atgatcatga gcgccgatgc gctgctccgc
      481 cgtaacgcaa accccagcga ggacgagatc cgcaaagcgc tcggcggcaa tctgtgccgt
      541 tgcaccggct actcgaagat cgtcgagtcg gtgcagtgcg cgtgcgcggg aagggagggc
      601 tgatggatac gaccgtgaac gtgatcgcgg ccgacatgct gcgcgaaggc agcatcggct
      661 gtccgacgcc gctcatcgac ggcgtcgaga aagtcaccgg ccgcgcgcgc tacaccgcgg
      721 acctgccggc aaccgacgcg ctcgccggcg cgatcctgcg cagccccgtg gcgcacggcg
      781 tcatccgcgg catcgacacc tcgcgtgcac gtgcgctgcc cggcgtgcgg gcggtcgtca
      841 ccggccagga tttcgtcgcg ccgtacggcg tgattccgat cgcgcagaac gaatggccgc
      901 tcgcgcgcga tcgcgtccgc tatcgcggcg agccggtggc ggccgtcgcc gccgtcgacc
      961 gcgcgaccgc gcaagccgcc ctcgcggcca tcgtgctcga catcgagccg ctgcccgcgt
     1021 acttcgcggc ggccgatgca cgcgccgaag gcgcggtgct cctgcacgac aggaagccgg
     1081 gcaacgtcga gcgcgaagtc gaccacgtgt tcggcgacgt cgacgcgggc tttgccgcag
     1141 ccgatctcgt gcgcgagcag accttccact atgccgaggt cgcgcacggg cagatcgagc
     1201 gcaacggggc gctcgcgacg tacgagccgg agcacgcgcg gctcacgatg caatcgacga
     1261 cgcaagtgcc gtattacctg catctgacgc tcgcgcaatg cctcggtctc gacggcgcgc
     1321 aagtgcgcgt gatcaagccc ttcgtcgggg gcggcttcgg ccatcgcgtc gagcctctca
     1381 atttcgagat ggttgcgggc gcgctcgcgc gcgcggccgg gggcgtcgtg cgcgtcgagc
     1441 agtcgcgcga ggacagcttc ctcacgcatc gcgggcgtcc cgagactgac atccggctga
     1501 agctcggtgt caagagaacg ggcgagatca ccgccgtcga ttgcgagatc gtgcagcgcg
     1561 gcggcgcgta cgccggctac gggctcgtca cgatcctcta cgccggtgcg ctgttgcacg
     1621 cgctctacaa ggtgggcgcg gtccgctacc gcggctatcg gatctatacc aacacgccac
     1681 cgtgcggcgc gatgcgcggc cacggtgcgg tcgatgcgcg ccatgccttc gaagcgatgc
     1741 tcgacgcgat ggcgagcgaa ctcgggctcg atccgatcgc gctgcgccgt gcgaacctca
     1801 tcgagccgcc gtatcgcacg ctgaacgacc tgcaggtgaa ttcgtacggc attccggaat
     1861 gcctcgactg ggtcgagcgc gcgtccggct ggcgcgagcg tcacggcaag cttccccgcg
     1921 gtcgcggcct cgggctcgcg tgctcgcatt acgtgtcggg ctcggcgaag cccgtgcact
     1981 ggagcggcga gccgcacgcg gtcgtgaacc tgcggctcga cttcgacggc ggcatcacgc
     2041 ttctcacggg ggccgccgac atcgggcagg gcagctcgac gctgctcgcg caggtcgcgg
     2101 ccgaagtgct gctgctgccg ttgtcgcgca tccgcgtgat cgccgccgac agcgcgctca
     2161 cgccgaagga caacggctcg tactcgtcgc gcgtgtcgtt catggtcggc aatgcggcga
     2221 tgcgcgccgc gcaggcgctg ctcgacgtgc tcgtcgcggc agcggcaagg aagctcgacg
     2281 tcgcgccggc cgagatcgaa tggctgggcg aggcgtgccg ggtgcgcggc ggggagcgcg
     2341 cgctcgactt cgcggccgtc gtcacggcgg cgctcgcgga caatgggccg gtcacggtca
     2401 aggggacgtg gtcggcgcca ccg
//
LOCUS       DASSNN010000217         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_172138, whole genome shotgun sequence.
ACCESSION   DASSNN010000217 DASSNN010000000
VERSION     DASSNN010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172138"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFL84_03990"
     CDS             complement(<1..435)
                     /locus_tag="VFL84_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7607813.1"
                     /translation="MANIGKGRSEDASSGPVRIGVISDTHGLVRPEAERALTGCELLI
                     HAGDVGKPEVLDKLRRLAPVRAIRGNIDKWAADLPDTEVVEIGAHCLYVLHDLNELEL
                     DPAAAGFAAVISGHSHRPRIERREGVLYLNPGSAGPRRFKLPV"
     gene            complement(545..1939)
                     /locus_tag="VFL84_03995"
     CDS             complement(545..1939)
                     /locus_tag="VFL84_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7607814.1"
                     /translation="MKLPALVRTSVFQLTIVYMALFAASVIALFAFIHWSTIGYLQRQ
                     ADAVIQAEVAGLREQAERGALLGLIDVINERLQDDTDGRSIYLLADAAGLPLAGNVIG
                     GWPRGLPFREGWVDSLAVRNHLGGTTPVRAQVLAVGPGFHLMVGRDVRELVRINEVFR
                     RASLWGIGLTMGLSLVGGLLVGLSAQRRIAQINRTTRQIIAGNLSQRVPVQGSRDEHD
                     ELARNVNAMLDQIETLLEGIRHVGDSIAHDLRGPLTRLRNRLDTLAQQPAPTHDDVAD
                     CVAQADRLLVTFNALLRIARVESGAYRSEFADVDLAQIVRDVCELYQAAAEEHHIRLS
                     CAPLAPNAIVFGDRELLAQALTNLLDNAVKYTPSGGRIDVRLVCSDNPAGYSLSIGDT
                     GPGVPPEERDRVLQRFARLDQSRTMPGNGLGLALVKAIAEQHDAELKLGDNEPGLVVT
                     LRLPAAGAATAEIA"
     gene            complement(1965..2648)
                     /locus_tag="VFL84_04000"
     CDS             complement(1965..2648)
                     /locus_tag="VFL84_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7607815.1"
                     /translation="MRVLIIEDDKDVASFLQKGLAQSGWNVDVAENGRDGLFLATTES
                     YHALIVDRMLPGVDGLTVVRTLRASNDTTPALILSALGEVDDRVKGLKAGGDDYLVKP
                     FAFSELTARLEALIRRSKAGASSAATVLDVGDLHMDLLRREVHRGGKKIDLQPREFQL
                     LEFLMRHAGQVVTRTMLLEGVWDYHFDPQTNVIDVHISRLRSKIDRGFDTPLLHTVRG
                     VGYRLDEQG"
BASE COUNT          471 a          919 c          973 g          475 t
ORIGIN      
        1 tacgggcagc ttgaagcgcc gcggccctgc gctgccgggg ttcaggtaca gcacgccttc
       61 gcggcgttcg atgcggggcc gatgcgagtg gcccgatatc acggccgcga acccggccgc
      121 ggccggatcc agctcgagct cgttgaggtc gtggagcaca tagagacagt gtgcgccgat
      181 ttcgacgacc tcggtgtcgg ggaggtcggc ggcccatttg tcgatgttgc cgcggatggc
      241 gcgcacgggc gcgagacgtc gcagcttgtc gagcacctcg ggcttgccga cgtcgccggc
      301 atgaatcagg agctcgcagc cggtcagcgc ccgttcagcc tcgggtcgca cgaggccgtg
      361 cgtatcggag atcacgccga tgcgtaccgg gccgcttgaa gcgtcttcgg agcgcccttt
      421 cccgatgttc gccatgatcg atatggtacc cggtaccttc ggagcgccga ccgtctcgat
      481 ctcgaggatg cgaccgccgg caagatcgaa gcaagtagca gaacgccgca gccgcgcccg
      541 tctctcacgc gatctcggcc gtcgccgcgc cggccgcggg cagtcgcagc gttacgacta
      601 aaccgggttc gttgtcgccg agcttgagct ccgcgtcgtg ctgctcggcg atcgccttca
      661 cgagcgcgag gcccaggccg ttgccgggca tcgtgcgcga ctgatcgagt cgggcgaagc
      721 gctggagcac gcggtcgcgc tcttcggggg ggacgccggg gccggtatcg ccgatgctga
      781 gcgagtagcc ggcgggattg tccgaacata cgaggcgaac gtcgatgcgg cctccgctcg
      841 gcgtatattt caccgcattg tcgagcaggt tcgtcagcgc ctgcgcgagc agctcgcgat
      901 cgccgaacac gatggcattc ggcgcgaggg gcgcgcagct caaccggatg tgatgctcct
      961 cggcggcggc ctggtagagc tcgcagacgt cgcgcacgat ctgtgccaaa tccacgtccg
     1021 cgaactcgct gcggtaagcg ccggattcca ccctcgcgat gcgcaacagc gcgttgaagg
     1081 tgacgagcag ccggtccgcc tgggccacgc agtcggcgac gtcgtcatgg gtcggcgcag
     1141 gctgctgagc gagggtgtcg agacggttcc ggagccgcgt caaaggtccg cgcagatcgt
     1201 gggcgatgct gtcgccgaca tgtctgatgc cctcgagcag cgtttcgatc tggtcgagca
     1261 tcgcgttgac gttgcgggcg agctcgtcgt gttcgtcgcg gctgccctgc accggcacgc
     1321 gctggctcaa gttgccggcg atgatctgcc gggtcgtccg gttgatttga gcgatgcggc
     1381 gttgggcgct gagcccgacg agcaggccgc cgacgagcga gagacccatg gtgagaccga
     1441 tcccccagag cgatgcgcgg cgaaagacct cgttgatcct cacgagctcg cgaacgtcgc
     1501 gtccgaccat gagatggaag cctgggccta cggccagcac ctgcgcgcgg accggagtgg
     1561 tccctccgag gtggttgcgc acggccaacg aatcgaccca gccttcgcgg aaaggaaggc
     1621 cccggggcca gccgccgatg acatttcctg cgagcggcag cccggccgcg tcggcgagga
     1681 ggtagatgga gcgcccgtcg gtgtcgtcct gcaagcgttc attgatgacg tcgatgagcc
     1741 cgagcagggc gccgcgctcg gcctgctccc ggagcccggc gacttcggcc tgaatcaccg
     1801 cgtccgcttg cctttgcagg tagccgatcg tggaccagtg aatgaatgcg aacagcgcga
     1861 taacggacgc cgcgaacagc gccatgtaca cgatcgtgag ctggaaaaca ctcgttctaa
     1921 cgagcgccgg cagcttcatg cggcgcagac gcctcagcgc ggcgtcaacc ctgctcgtcc
     1981 agccggtagc cgacgccgcg aaccgtgtgc agcagcggcg tatcgaagcc gcgatcgatc
     2041 ttcgagcgta ggcggctgat gtggacatcg atgacattcg tttgaggatc gaaatgataa
     2101 tcccatacgc cttcgagcaa catcgtccgg gtcacgactt gcccggcgtg gcgcatgagg
     2161 aactcgagca gctggaactc gcgcggctgg agatcgatct tcttgccgcc gcgatggacc
     2221 tcgcgccgca gcagatccat gtgcaggtcg ccgacgtcga gcaccgtcgc ggccgaagag
     2281 gctccggcct tggaacgccg gatcagggcc tcgagacggg ccgtgagctc gctgaaggcg
     2341 aagggcttca cgagatagtc gtcgcctccg gccttgagcc ccttgacgcg gtcgtcgacc
     2401 tctccgagcg cactcaaaat caacgccggg gtcgtgtcgt tggaggccct cagggtccgc
     2461 acgacggtca agccgtcgac gccgggcagc attcggtcga cgatgagcgc gtgatagctc
     2521 tccgtcgtgg cgaggaaaag gccgtcgcgg ccgttctccg ccacgtccac gttccagccg
     2581 gactgcgcga gtcctttctg gaggaacgag gcgacgtcct tgtcgtcctc gataatcaat
     2641 acacgcaatt ctctgggctc cgcagaacgc ccggcggccg ttcgagcgcc cgcccggctc
     2701 agttgtagat gcacctcata gagtaacaag ggtcgccgcg cgggcaacgt tcggatcgag
     2761 gctgcggcct cggccgcgcc ggccgcagct tcgacgacac gctgccgccg cttcggccgc
     2821 gtcgaacttt gcagaaat
//
LOCUS       DASSNN010000218         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1791141, whole genome shotgun sequence.
ACCESSION   DASSNN010000218 DASSNN010000000
VERSION     DASSNN010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791141"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /gene="pstC"
                     /locus_tag="VFL84_04005"
     CDS             complement(<1..1382)
                     /gene="pstC"
                     /locus_tag="VFL84_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET7607816.1"
                     /translation="MASSTLLFVVVGLALLGYYVARQRAVSSAVAAGPRSFHSLPGYH
                     GGYVALWCALPALALLVLWQVLEPVWLRSNVLDSLPETTRALPEDQLGLVYNEIRNLV
                     DGDFVVGEPTPELAAAAERYTELRASSRKMATAAVLVLGVLVLVWAYRRVRPDFRARN
                     RVERAIELLLVAASCVAILTTIGIFLSVLVEALRFFRQVSLLDFLFGTSWSPQTAIRA
                     DQIGASGAFGAVPLFAGTLLISGLAMLVAVPVGLMSAIYLSEYASRRFRALAKPLLEI
                     LAGIPTVVYGYFAALTVAPLLRGLGESIGLDVAAESALGAGLVMGIMIIPFVSSLSDD
                     VINAVPQALRDGSYGLGATKSETIRNVVFPAALPGIVGAVLLAVSRAIGETMIVVMAA
                     GLAANLTANPFEAVTTVTVQIVMLLTGDQEFDSAKTLAAFALGLVLFCVTLILNVIAL
                     RVVRRYREKY"
     gene            complement(1428..>2335)
                     /locus_tag="VFL84_04010"
     CDS             complement(1428..>2335)
                     /locus_tag="VFL84_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7607817.1"
                     /translation="GRRNPRFKTPKIEPTGSGGGIKAFCGGVGVQFPDIANSSRRITA
                     SEVADCGKNGVTAIAEIKIGYDGIVLANAKTSPHYQLTLRDLYLALAKEVPDRSGAEK
                     LVPNPYTRWSEINPALPADEISVLGPPPTSGTRDAFNELVMEGGCKTFAWVAALPREQ
                     YLTACHLLRDDGKYTDAGENDNLIVQKLEASPRQVGIFGFSFLEQNSDRVRGAAIDGV
                     APTFDTIADGRYPVSRPLYFYVKKAHVGRVPGIREYVAEFVSDAAAGELGYLADIGLI
                     PLGDDEREAMQAQAKNLGDVDVADH"
BASE COUNT          418 a          771 c          795 g          351 t
ORIGIN      
        1 tcatattttt ccctgtatct gcgaaccacg cgcagggcga tgacgttgag gatgagcgtg
       61 acgcagaaga gcacaaggcc gagcgcgaag gcggccagcg tcttggcgct gtcgaattct
      121 tgatcgcccg taagcagcat cacgatctgc accgttaccg tcgtcacggc ctcgaacggg
      181 ttcgccgtca ggttcgcggc cagccccgcg gccatcacga cgatcatcgt ctcgccgatc
      241 gcgcgcgaca ccgcgagcaa cacggcgccg acgatgccgg gcagcgccgc gggaaacacg
      301 acgttgcgaa tcgtctccga cttcgtcgcg ccgaggccgt acgagccgtc gcgcaacgct
      361 tgcggcacgg cgttgatgac gtcgtccgac aacgacgaga cgaacggaat gatcatgatg
      421 cccatgacga ggccggcgcc gagcgcgctt tccgccgcga cgtcgagccc gatgctctcg
      481 ccgaggccgc gcagcaacgg cgcgaccgtc agcgccgcga agtagccgta cacgaccgtc
      541 ggaatgccgg cgaggatctc gagcaacggc ttcgcaagcg cgcgaaagcg ccggctcgcg
      601 tactcggaaa ggtagatcgc ggacatgagc ccgacgggaa cggcgacgag catcgcgaga
      661 cccgagatca acaacgtgcc cgcgaagagc ggcaccgcgc cgaacgcgcc cgaagcgccg
      721 atctggtcgg cgcggatcgc cgtctgcggg ctccacgacg tgccgaacag gaagtcgagc
      781 agcgagactt gccggaagaa gcgcaacgct tcgacgagca cggagaggaa gatgccgatc
      841 gtcgtcagga tcgccacgca cgacgccgcg acgagcagca gctcgatggc tctctcgacg
      901 cggttgcgcg cgcggaagtc gggccgcact cgccgatacg cccacacgag aacgagcacg
      961 ccgagcacga gcacggcggc cgtcgccatc ttgcggctcg aggcgcgcag ctccgtgtag
     1021 cgctcggccg cggccgcaag ctcgggtgta ggctcgccga cgacgaaatc gccgtcgacg
     1081 agattgcgga tttcgttgta gacgagcccg agctggtcct cggggagcgc ccgcgtcgtc
     1141 tcgggcagcg aatcgagcac gttgctgcgc agccaaacgg gctcgagcac ttgccacagc
     1201 acgagcagcg ccagcgcggg caaagcgcac cacaacgcga cgtagccgcc gtgatagccg
     1261 ggcagcgagt gaaagctgcg cggacccgct gctacggcgg agctgacggc acgctgacga
     1321 gcgacgtagt agccgagcag agccaggccg acgacgacga acaacagtgt cgatgaagcc
     1381 acgcagcgtt ctttgggtta gcgcgaatga cgggtcatcg tactcggtca atgatcggcg
     1441 acgtcgacgt cgccgaggtt cttcgcttgc gcctgcatcg cctcgcgctc gtcgtcgccg
     1501 agcgggatca aaccgatgtc ggcgagatag ccgagctcgc cggcggccgc gtcgctgacg
     1561 aactccgcga cgtactcgcg gataccgggg actcgcccga cgtgcgcctt cttgacgtag
     1621 aagtagagcg gccgcgatac ggggtagcgg ccgtccgcga tcgtgtcgaa cgtcggcgcg
     1681 acgccatcga tcgctgcgcc tcgcacgcgg tccgagtttt gctcgaggaa gctgaagccg
     1741 aagatgccga cctggcgcgg gctcgcctcg agcttctgca cgatcaggtt gtcgttctcg
     1801 ccggcgtcgg tgtatttgcc gtcgtcgcgc agcaggtggc aagcggtgag gtattgctcg
     1861 cgcggtagcg ccgcgaccca cgcgaacgtc ttgcagccgc cctccatgac gagctcgttg
     1921 aacgcgtcgc gcgtgcccga cgtcggcggc gggccgagca cgctgatctc gtcggccggc
     1981 agcgcgggat tgatctccga ccatcgcgtg tacggattcg gcacgagctt ctccgcgccc
     2041 gaacgatcgg gcacttcttt cgcgagcgcg aggtacaagt cgcgcagagt gagctggtag
     2101 tgcggcgacg tcttcgcgtt cgcgagcacg atgccgtcgt agccgatctt gatctcggcg
     2161 atcgccgtaa cgccgttctt gccgcagtcg gccacctcgc tggccgtgat gcgccgcgac
     2221 gagttcgcga tgtcggggaa ctggacgccg acgccgccgc aaaacgcttt gatgccgccg
     2281 ccgctgcccg tcggctcgat cttcggcgtt ttgaagcgcg gattgcggcg gccga
//
LOCUS       DASSNN010000219         3564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1429012, whole genome shotgun sequence.
ACCESSION   DASSNN010000219 DASSNN010000000
VERSION     DASSNN010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429012"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..399)
                     /locus_tag="VFL84_04015"
     CDS             complement(61..399)
                     /locus_tag="VFL84_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET7607818.1"
                     /translation="MQLSEEGPVAVNNATFAKTVLAAPQPVLLDFGAEWCGPCVAVAP
                     VIRSLALEYADRLTVATVDVDADPQLAARYGVRSLPTVMLLESGEIRRVLVGARSGQE
                     YRAAIEGVLH"
     gene            720..1646
                     /gene="gcvA"
                     /locus_tag="VFL84_04020"
     CDS             720..1646
                     /gene="gcvA"
                     /locus_tag="VFL84_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HET7607819.1"
                     /translation="MRNQRYDVPPLDPLLAFEAAARNLSFTKAASELFLTQSAVSRQI
                     QQLEEHLGVKLFERRARTLLLTENGQLFYRATQDALQKLHDSSRRLRGTTKVNTVVVA
                     TTPGFASLWLIPHLAGFTRDHSAVDVRISATNELIEDLERAHVDVAVRYVPEGSAGGR
                     LLFGETVFPVCNPELAKKLVTPDDLHQHVLLYLEHPRAAWLDWELWFHALGLRDFEPL
                     GKLHFSHYDQLIQAAIDGHGVALGRHPLLRQLLREGKLVAPFKDKVASSRAYYVVESP
                     AAKGKAPVREFADWLVEATQAETLDAPAGEAA"
     gene            complement(1656..1976)
                     /locus_tag="VFL84_04025"
     CDS             complement(1656..1976)
                     /locus_tag="VFL84_04025"
                     /inference="COORDINATES: protein motif:HMM:NF022459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HET7607820.1"
                     /translation="MRAAIVFFAVLGVAFSASGEDTIRCRNGRLVNVGMTAAEVTSRC
                     GEPTSRTVEEIPVMTRLPTGAVRQTGTTHAERWIYARGQGQFDAMLTFEDGKLRRIDL
                     VTEP"
     gene            complement(1976..>3564)
                     /locus_tag="VFL84_04030"
     CDS             complement(1976..>3564)
                     /locus_tag="VFL84_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HET7607821.1"
                     /translation="IGHAKAICIDFGIAREFGGTCYLRFDDTNPERETEEFVESIKRD
                     VRWLGFDWGDRLRHASDYFKDIHACAVELIKRGLAYVDHQNAEEIRKNRGTLTEPGKN
                     SPYRDRSVEENLELFEAMRRGDFEEGACVLRAKIDMASPNINLRDPTLYRIRKVAHQR
                     TGGEWCIYPMYDYAHTLSDAIEGTTHSLCTLEFEDHRPLYDWFLDQLWQGKPSRPRQI
                     EFSRANLMFTVTSKRKLKQLVDDKHVEGWDDPRMPTLAGIRRRGYTPESIVEFAHRIG
                     TTKKEKVAELGLLEGCVREHLNDTAPRRFAVLDPLKIVIETYPESKEEWLDAANHPNR
                     PELGLRKVPFAREILIERDDFMENAPSKFHRLKPGGEVRLRYGYIVKCERVVKDAAGN
                     VVELRCSHDAASRSGSDESGRKVKGTIHWVSAKHAGRAPVRLYDRLFSVPFPGDNLAK
                     ELNPESLKIVSSAALEPALLEALPEERFQFERLGYFVADSRLSKPGAPVFNRTVTLRD
                     SWAKVEQEALAALPRDKEKS"
BASE COUNT          587 a         1185 c         1129 g          663 t
ORIGIN      
        1 aaccacgcaa acgttgggac aggcctgatc tgtccccatt ttcagggctt caagagaatt
       61 ctagtgcagc acgccttcga tcgccgcgcg gtattcctgg cccgagcgag cgccgaccag
      121 cacgcgccgg atctcgccgc tctcgagcag catgaccgtc ggcaacgaac gcacgccgta
      181 gcgcgccgca agctgcgggt ccgcatcgac gtcgacggtc gcaaccgtca accgatcggc
      241 atactcgagc gccaagctgc ggatcacggg cgcaacggcc acgcagggcc cgcaccactc
      301 ggcgccgaaa tcgaggagca ccggttgcgg cgctgccagc acggtcttcg cgaacgtcgc
      361 gttgttgacg gcgaccggac cttcttcact gagctgcatg aggcctccct tcctttcgcc
      421 gcggatcaac cggcgccgtt caaagcgagc acagcgctca ccacggcagc caagacggcc
      481 gtcgccaaca acagctcggg gatcgattct ccaaacagct cgcgcggccg gaccaatatc
      541 ttggcggcag gacgcgagaa cctgcggcca aaaagcactc gagtcgtcat ttccgtctcc
      601 tattcatgag atttcagaat gccattcagc tggcatttct ggtttcacgg cggccataat
      661 cgtgcgcttc cagtgcggtc acaaacggtc tttgcgcatc ttatcgatgc gttttttgca
      721 tgcgtaacca gcgctacgac gttcccccgc tcgacccatt gctcgcgttc gaggcggctg
      781 cccgtaacct gtcgttcacg aaggcggcga gcgagctatt tcttacgcag tcggccgtga
      841 gccggcagat tcagcagctc gaggagcact tgggggtaaa gctgttcgag cgccgcgcgc
      901 ggaccttgct gctgacggaa aacggccagc tgttctatcg cgcaacgcag gacgcgctgc
      961 agaagctgca cgacagctcg cggcggctgc gcggcacgac gaaggtgaac acggtcgtcg
     1021 tcgcgacgac accggggttc gcctcgctgt ggctgatacc gcatctcgcg ggcttcacgc
     1081 gcgaccacag cgccgtcgac gtccgaattt cggcgacgaa cgagttgatc gaggatctcg
     1141 agcgagcgca cgtcgacgtt gccgtccgct acgtgcccga gggcagcgcc ggaggacggc
     1201 tgctgttcgg cgagacggtg tttccggtct gcaaccccga gctcgcgaaa aagctcgtga
     1261 cgcccgacga cttgcaccag catgtactgc tctatctcga gcatccgcgc gccgcgtggc
     1321 tcgattggga gctctggttt cacgcgctcg gccttcgcga cttcgagccg ctcggcaagc
     1381 tccacttcag ccattacgat cagctgatcc aggccgcgat cgacggtcac ggtgtcgcgc
     1441 tcggccggca tccgctgctg cgccagctcc tgcgcgaggg caagctcgtc gccccgttca
     1501 aggacaaggt cgcgtcgtcg cgcgcctatt acgtcgtcga gtcgccggcc gccaagggga
     1561 aagcaccggt gcgtgagttc gccgactggc tcgtcgaggc gacgcaggcc gagacgctcg
     1621 acgcgccggc cggcgaggcc gcgtgagggg tcgccctagg gttccgtgac gaggtcgatt
     1681 cgccgcagct tgccgtcctc gaacgtcagc atcgcgtcga attgaccctg gccgcgcgcg
     1741 tagatccagc gctctgcgtg cgtcgttccc gtctgcctta cagcgcccgt cggcagacgc
     1801 gtcatcacgg gaatctcctc gaccgtgcgg ctcgtcggtt cgccgcagcg gctcgtcacc
     1861 tccgcagcgg tcatgccgac attgacgagg cggccgttgc ggcagcgaat cgtgtcttcg
     1921 ccgcttgccg agaacgcgac gccgagtacg gcaaagaaaa cgatcgcggc tcgcatcacg
     1981 atttctcctt gtcacgcggc aacgccgcga gcgcctcctg ctcgaccttg gcccacgagt
     2041 cgcgcagcgt gacggtgcgg ttgaagaccg gcgcgccggg cttcgacagc cgtgaatcgg
     2101 ccacgaagta gcccaggcgc tcgaactgga atcgctcttc gggcagcgcc tcgagcaatg
     2161 caggctcgag cgccgcgctg ctcacgattt tcagcgactc cggattcagc tccttcgcca
     2221 ggttgtcgcc ggggaacggc accgaaaaga gtcgatcgta caggcgcacg ggcgcgcggc
     2281 ccgcgtgctt cgccgagacc caatggatcg tgcccttgac cttgcggcca gactcgtcgg
     2341 agccgctacg cgacgcggcg tcgtggctgc accgaagctc cacgacgttg ccggccgcat
     2401 ccttgacgac gcgctcgcac ttgacgatgt agccgtagcg caagcggact tcgcctcccg
     2461 gtttcaggcg atggaacttg ctcggcgcgt tctccatgaa gtcgtcgcgc tcgatgagta
     2521 tctcgcgcgc gaacggcacc tttctgagcc cgagctccgg ccggttcggg tggttggcgg
     2581 cgtcgagcca ttcctcttta ctctcgggat aggtctcgat gacgatcttc agcggatcga
     2641 gcacggcgaa acgccgcggc gccgtgtcgt tcaagtgctc acgcacgcac ccttcgagca
     2701 ggccgagctc cgcgaccttt tctttcttgg tcgtgccgat gcggtgcgcg aactcgacga
     2761 tcgactccgg cgtgtagccg cggcggcgga taccggccag cgtcggcata cgcgggtcgt
     2821 cccagccttc gacgtgcttg tcgtcgacga gctgcttcag cttgcgcttg ctcgtcaccg
     2881 tgaacatcaa gttcgcgcgc gagaactcga tctgccgcgg ccggctcggc ttgccctgcc
     2941 agagctggtc caagaaccag tcgtagagcg gccggtgatc ctcgaactcg agcgtgcaca
     3001 gcgagtgtgt cgtgccttcg atcgcgtcgg agagcgtgtg cgcgtagtcg tacatcggat
     3061 agatgcacca ctcgccgccc gtgcgctggt gcgcgacctt gcggatgcga tagagcgtcg
     3121 gatcgcgcag gttgatgttc ggcgacgcca tgtcgatctt ggcgcgcagc acgcacgcgc
     3181 cttcttcgaa gtcgccgcgc cgcatcgcct cgaacagctc gaggttctcc tcgaccgagc
     3241 ggtcgcggta cgggctgttc ttgccgggct cggtcagcgt gccgcggttc ttgcggattt
     3301 cttcggcgtt ctggtgatcg acgtaagcga ggcctcgctt gatcagctcg acggcgcagg
     3361 cgtgaatgtc tttgaaatag tcggacgcgt gccgcagccg atcgccccag tcgaagccga
     3421 gccagcgcac gtcgcgcttg atgctttcga cgaattcctc ggtctcgcgc tcggggttcg
     3481 tgtcgtcgaa tctgagatag cacgtgccgc cgaattcgcg cgcgatgccg aaatcgatgc
     3541 aaatggcctt cgcgtggccg atgt
//
LOCUS       DASSNN010000220         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_546735, whole genome shotgun sequence.
ACCESSION   DASSNN010000220 DASSNN010000000
VERSION     DASSNN010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546735"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFL84_04035"
     CDS             <1..627
                     /locus_tag="VFL84_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HET7607822.1"
                     /translation="PTTWINASAIGYYGTRDGDDALNEKSGTGSGFQADLCRRWEETA
                     SRAAEHGVKVCTLRIGVVLSCDGGALPALARPVRFLAGTLLGSGRQWFSWIHIDDLLD
                     VISFVLAEATLAGPVNATAPVPVRHDELMTAIAAALRRPLWRVHVPAWLLRFALGELA
                     ELFVDGQRVTPNRLLALKFRFRYENVDAALERALATPAHAAPAVKGAP"
     gene            complement(629..1117)
                     /locus_tag="VFL84_04040"
     CDS             complement(629..1117)
                     /locus_tag="VFL84_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607823.1"
                     /translation="MWSDRAALLALVALSVLPAAAFEDLRALEVQGLRGQSAEQARRD
                     RYECHNWALAQSGQAPPAAPVAAPPADAKADLKHERIGRAIVGATIGGTIGSLFGDRH
                     DANESVLAGAAAGAAIGAATAGAGSKNEQPPAPEGPSDYLRALTACLTGRGYSVALPD
                     LG"
     gene            complement(1104..1532)
                     /locus_tag="VFL84_04045"
     CDS             complement(1104..1532)
                     /locus_tag="VFL84_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607824.1"
                     /translation="MRALLIAVVGAAAFWSVPAEASHWHVWPYFGFGYGAGPYPFYGP
                     GYGPHIGVGVHVGRAYPAHPRPAPRAGQPAPQGALKLYVYPAAGQSEAQTADDRYQCH
                     VWAAGQSGYDPTLGAGSRDEAEGYTRAFTACMEGRNYVVR"
     gene            1611..>2009
                     /locus_tag="VFL84_04050"
     CDS             1611..>2009
                     /locus_tag="VFL84_04050"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7607825.1"
                     /translation="MSTSLRGACLCGAIAFAAAPPYRWFAHCHCSMCRKHYGSLFGTS
                     LGVLRDAFRWLEGSDAIVHYRATPAFERPFCRHCGSTVPGASEDEGCLNVPAGLLIGD
                     LGARPRSHIFVASRSPLTTLDDALPRHDAYP"
BASE COUNT          279 a          731 c          698 g          301 t
ORIGIN      
        1 ccgacgacgt ggatcaacgc ttccgcgatc ggctactacg gcacgcgcga cggcgacgac
       61 gcgctgaacg agaagtccgg caccggcagc ggattccaag ccgacctgtg ccggcgctgg
      121 gaggaaaccg cgtcgcgcgc cgcggagcac ggagtcaaag tctgcacgct gcgaatcgga
      181 gtcgtgctgt cctgcgacgg cggcgcgctg cccgcgctcg cgcgccccgt gcgttttctg
      241 gcgggcacgc tgctcggatc gggccgccaa tggttctcgt ggatccacat cgacgacttg
      301 ctcgacgtga tctcgttcgt gctggcggag gcgacgctgg cggggccggt caacgcgacg
      361 gcgccggtgc cggtccggca cgacgagctc atgacggcga tcgcggcggc cctacggcgg
      421 ccgttgtggc gcgtgcacgt gccggcatgg ctgctccgct ttgcgctcgg cgagctcgcg
      481 gagctgttcg tcgacggtca acgcgtgacg ccgaatcggc tgctcgcgct gaagttccgg
      541 ttccgctacg aaaacgtgga cgccgcgctc gagcgagcgc tcgcgacgcc cgcgcacgcg
      601 gcgccggccg tcaaaggcgc gccgtgagct agcctaagtc gggcagcgcc acggagtagc
      661 cgcggcccgt aagacacgcg gtcagcgcgc gcagatagtc gctcggccct tcgggcgccg
      721 gcggctgctc gttcttgctt ccggcgccgg ccgtcgcggc accaatcgcg gcgccggcgg
      781 ccgcgcccgc gaggacgctc tcgttcgcgt cgtgtcgatc gccgaacagg ctgccgatcg
      841 taccgccgat cgtggctccg acgatcgcgc gaccgatgcg ctcgtgcttc aggtcggcct
      901 tggcgtccgc cggcggtgcc gcaaccggcg ccgcgggcgg cgcttgcccc gactgcgcga
      961 gggcccagtt gtggcattcg taacgatcgc ggcgcgcttg ctcggccgac tggccgcgca
     1021 gcccttggac ctcgagcgcg cgcaggtcct cgaaggcggc cgccggcagc accgaaagcg
     1081 cgactaacgc gagcagcgcc gccctatcgg accacatagt tgcgcccttc catgcaggcc
     1141 gtgaacgcgc gcgtatagcc ttcggcctcg tcgcggctgc ccgcgccgag cgtcggatcg
     1201 tagcccgatt ggccggcggc ccagacatga cactgataac ggtcatcggc cgtctgcgcc
     1261 tcgctctgac cggccgcggg atagacgtag agcttcagcg cgccttgcgg cgccggctgc
     1321 cccgcgcgcg gcgccggcct cgggtgcgcg ggataggccc tgccgacgtg aacgccgact
     1381 ccgatgtgcg ggccgtagcc gggcccgtag aaaggatacg gacccgcgcc gtagccgaag
     1441 ccgaagtacg gccagacgtg ccagtggctc gcctcggccg gtacactcca aaacgccgca
     1501 gcgccgacga ccgcaatgag caacgcgcgc atcgatccac tccctcgact tcgggcagac
     1561 caccccttgc taccgacgat agccgccgtg actgaacggc agctgaaagc atgagcacga
     1621 gccttcgcgg cgcctgcctc tgcggcgcga tcgcgttcgc ggccgcgccg ccgtaccgct
     1681 ggttcgcgca ttgccattgc tcgatgtgcc gcaagcacta cggaagcctg ttcggcacga
     1741 gcctcggcgt cttgcgcgat gcgtttcgct ggctcgaggg cagcgacgcg atcgtgcact
     1801 atcgcgcgac gcccgcgttc gagcggccgt tttgccgcca ctgcggctcg acggtgccgg
     1861 gcgcttcgga agacgaaggc tgcttgaacg ttccggccgg gctcctcatc ggcgacttgg
     1921 gcgcacgccc gcgctcgcac atcttcgtcg cgtcgcgctc gccgctgacg acgctcgacg
     1981 acgcgctgcc gcgccacgac gcctacccg
//
LOCUS       DASSNN010000221         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1429090, whole genome shotgun sequence.
ACCESSION   DASSNN010000221 DASSNN010000000
VERSION     DASSNN010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429090"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFL84_04055"
     CDS             <1..783
                     /locus_tag="VFL84_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7607826.1"
                     /translation="KQGRAFATTDTADAPRVAVVNEALANRFWPGESAVGHRFRGADG
                     SWTEIVGVVETGPYFTIVEGPRAFVYLPYAQAPQSQMTLITRSSADPLALVDPLRAVV
                     RELDPALAVAAVRTMESLYNDSAVRGFMVFIYAIAAMVVMSLTLAFGGIYGLVASNVS
                     QRTREIGLRIAVGADRRNVLRMVLGHALRVTLIGLVLGVLLTFGAEQVMRASFPGNDG
                     AGREVIEYVRVIAVMLLVAGLAAYLPARRATRIEPTQALRHQ"
     gene            complement(875..1267)
                     /locus_tag="VFL84_04060"
     CDS             complement(875..1267)
                     /locus_tag="VFL84_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607827.1"
                     /translation="MLLLLLTFGGNLGCKLVLRWSATKPPPESGEKITLRAGRVIGIL
                     ERLLIFLGLVASSWEILAGVVALKTVARYSDLDKQDKAEYFLIGSLASILWAVVVTAL
                     IAFYDLRWGFGALPSFVGLLGNGASSPP"
     gene            complement(1300..1965)
                     /locus_tag="VFL84_04065"
     CDS             complement(1300..1965)
                     /locus_tag="VFL84_04065"
                     /inference="COORDINATES: protein motif:HMM:NF027589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HET7607828.1"
                     /translation="MPSKKPLPLAVLMGDIVGSERARSVKTVHRVFNKAIDFANERYA
                     DGIESPLTITLGDEFQGLLRTLSHAWRVACDLRFRLLVANISCRFVIGTARLETPVNT
                     REAWNMMGGGLAEAREKLNDKRTGNAYRFSVPEKPIIESLLDAVGDSLTEVETAWTAT
                     QLEYYRAVHDSARSKAAVAKSLGVSARSLFKVLRAARAEFHGRQSAILARALAALDER
                     YGL"
BASE COUNT          362 a          617 c          660 g          371 t
ORIGIN      
        1 aagcaaggcc gcgcatttgc gacgactgat acggccgatg cgccgcgtgt cgccgtcgtc
       61 aacgaagcgc tcgcgaatcg gttctggccg ggagagagcg ccgtcgggca tcgcttccgt
      121 ggcgccgacg gaagctggac agaaattgtc ggtgtggtcg agaccggacc ttacttcacg
      181 atcgtggagg ggccgcgggc gttcgtctac ttgccttacg cgcaggctcc gcagagccag
      241 atgacgctca tcacgcgatc gagcgccgat ccgctcgcgc tcgtggaccc gttgcgcgcc
      301 gtcgttcgcg agctcgaccc ggccctcgcc gtggcggcgg ttcgcacgat ggagtcgctc
      361 tacaacgatt cggcggtgcg tggcttcatg gtcttcatct atgcgatcgc cgcgatggtg
      421 gtgatgagcc tgacgttggc tttcggcggc atctacgggc tcgtcgcatc gaatgtcagc
      481 cagcgcacgc gcgagatcgg gttgcgcatt gccgtcggcg ccgatcggcg caacgtgctt
      541 cgcatggtgc tgggccacgc gttacgcgtg acgctgatcg gcttggtgct cggcgttttg
      601 ctcacgttcg gcgccgagca ggtgatgcga gcgtcgtttc cgggcaacga cggcgcgggc
      661 cgcgaggtca tcgagtacgt gcgtgtgatc gcggtgatgt tgctcgtggc ggggctcgca
      721 gcatacctgc cggcgcgccg cgcaacgcgc atcgagccga cgcaggcgct gcgtcatcaa
      781 tagcgagcgg tccatgacga cgcgtcggtc ctgaagtcgg ccttgcgcgt cgtgagcggg
      841 ggaagggcga gttgcggcgg gtcgaacttc gagctcatgg cggactgctg gcgccgttgc
      901 cgagtagccc gacgaacgac ggaagcgctc cgaaacccca gcggagatcg tagaacgcga
      961 tcagagcggt gacgaccact gcccacaaga tgctcgcgag cgatccgatc aggaagtact
     1021 cggccttgtc ctgtttgtcg aggtcgctgt aacgcgcgac cgtcttcaaa gcgacgacgc
     1081 cggccagaat ctcccagctg ctcgcgacaa gccctaggaa gatcagcaac cgctccaaga
     1141 tgccgatcac gcgcccggcg cgcagagtga tcttttcgcc gctctcgggc ggcggtttcg
     1201 tcgcgctcca gcggagcacc aatttgcagc cgaggttgcc tccgaaagtt agaaggagca
     1261 gcaacacggc atatccgctg attttcaacg cgagaatcgt cacagcccgt accgctcgtc
     1321 caatgccgcc aacgccctag cgaggatagc ggattgccgc ccgtggaact cggcgcgtgc
     1381 ggcgcgcaag accttgaaga gggaacgagc cgagactccc aagctcttcg cgacggcggc
     1441 tttcgagcgg gccgaatcgt gaacggcccg atagtactcg agttgcgtgg cggtccatgc
     1501 ggtctcgact tcagtcagtg agtcgcccac ggcatccagc aacgactcga tgatcggttt
     1561 ctcgggaaca gaaaatcgat aagcgttgcc ggtgcgcttg tcgttcagct tttcgcgggc
     1621 ctcggcgagg ccaccgccca tcatgttcca agcttccctg gtattgaccg gcgtctcgag
     1681 ccgggccgtg ccgatcacga atcggcagga gatgttcgca acgagcagac gaaaccgcag
     1741 atcgcaggca acgcgccagg cgtgtgacaa cgtgcggagc aggccctgga actcgtcgcc
     1801 gagcgtaatc gtcaacggtg actcgatgcc gtccgcgtag cgttcgttcg cgaaatcgat
     1861 tgctttgttg aaaacgcggt gaaccgtctt gacggagcgt gctctttcgc tcccgacgat
     1921 gtcgcccatc aggacggcaa gaggcaacgg cttcttcgac ggcatgactg aacctaattt
     1981 gggtcgctat gccgatagtg acccaaaaca
//
LOCUS       DASSNN010000222         3164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1791519, whole genome shotgun sequence.
ACCESSION   DASSNN010000222 DASSNN010000000
VERSION     DASSNN010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791519"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="VFL84_04070"
     CDS             complement(<1..1632)
                     /locus_tag="VFL84_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HET7607829.1"
                     /translation="MKSSSFACAALAAVLAACQSPPEPPSAAPTNASQTALWDAFRDE
                     YIEGYFRLNPSYAVYQGRHELDGQIGDWSEAGLARQIELFRQAIADARAFDAAQLSEA
                     QRFERDYLVHVARGELFWLADADAPHENLTWYFDNGLDPNVYIARPYADAATRLRAFI
                     GYLHAVPTAVAQLRANLETPMPLSFIEYGIAGFNGFAEYYRGDATAAFAGVGDAALQT
                     ELAAAVATAATVMTEAAEWLTSQRATASQDFALGAERFARMLEETEDVTVPLEELEAL
                     GRADLERNQQALTAACASFAPGAALGDCMARMDADKSPLGPVAEARAQLPALKQFIID
                     TGIVSVPGTEEALVEEAPPYNRQNSAYINIPGPYEKGMPSVYYIAPPDPAWTPEQQAA
                     YIPGSKNLLFTSIHEVWPGHFLNFLHSNRAPSLFGRVFVGYAFAEGWGHYAEELMWEA
                     GYESGNEETHIGQLSNALLRNCRYLSAIGLHARGMTQEQSRELFRTECYQDEGTARQQ
                     SARGTYDPAYLNYTMGKLMIRKLRADYTASRGGRAA"
     gene            1704..3062
                     /locus_tag="VFL84_04075"
     CDS             1704..3062
                     /locus_tag="VFL84_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7607830.1"
                     /translation="MTAAPKAIPALSRTLRSRHVALISLGGIIGAGLFVGSSANIASV
                     GPAVLVSYLVAGALIFLVMRMLAEMAAANPEALSFADHARAGLGSWAGFVCGWLYWYF
                     WVVVVGIEAIAGATILENWIPLPVWQIGLGLMVILTATNLLSTRSYGEFEFWFASIKV
                     AAILAFIAVAGSYALGLTSPAGATLGNLVDHGGFAPFGAAAVLAGVTGIVFSMTGAEI
                     VTVVAAESSEPSHAIAAMTTSLIARILLFYVGSVFCILAVVPWNEVVPGSSPFAQTLL
                     VIGIPFAATIMNLIVLVAVLSCLNSGLYITSRMLFGLAAQGDAPRALVALDKRLVPSR
                     AILIGTVFGYLAVLASVVSPQRLFTFLVNASGAIMLVIYLLIALAQIRRRHALEQGGE
                     KRLVIRMWLHPWLSLGTVLSIVLVLASMLFRPAERAQLAASALSVAVVLAAYAWKRLA
                     RR"
BASE COUNT          420 a         1062 c         1115 g          567 t
ORIGIN      
        1 cgccgcgcgg ccgccgcggc tcgccgtgta gtcggcgcgc agcttgcgga tcatgagctt
       61 gcccatcgtg tagttcaggt acgcgggatc gtacgtgccg cgcgcggatt gctggcgcgc
      121 cgtgccttcg tcttggtagc actcggtgcg gaacagctcg cgcgattgct cctgcgtcat
      181 gccgcgcgcg tgcaggccga tcgcagaaag ataccggcag ttgcgcagca gcgcgttcga
      241 gagctggccg atgtgggtct cctcgttgcc gctctcgtag ccggcctccc acatcagctc
      301 ctcggcgtag tgtccccagc cttcggcgaa cgcgtagccg acgaacacgc ggccgaacag
      361 cgacggcgcg cggttcgaat gcaggaagtt caggaagtgg ccgggccaga cctcgtggat
      421 cgacgtgaac agcagattct tgctgcccgg gatgtacgcg gcctgctgct cgggcgtcca
      481 ggccgggtcc ggcggcgcga tgtaatagac cgacggcatg cccttctcgt acggccccgg
      541 gatgttgatg taagccgagt tctggcggtt gtacggcggc gcttcctcga cgagcgcttc
      601 ctcggtgccc ggcacggaga cgatgccggt gtcgatgatg aactgcttca gcgccggcag
      661 ttgcgcgcgc gcctcggcca cggggccgag cggcgacttg tcggcgtcca tgcgcgccat
      721 gcagtcgccg agcgcggcgc ccggcgcgaa cgaggcgcac gcggccgtca gcgcctgttg
      781 attgcgctcg aggtccgcgc ggccgagcgc ttcgagctcc tcgagcggaa ccgtgacgtc
      841 ctccgtctcc tcgagcatgc gggcgaagcg ttccgcgccg agcgcgaagt cttggcttgc
      901 ggtcgcgcgc tgcgacgtaa gccactcggc ggcctcggtc atgacggtcg ccgcggtcgc
      961 gacggcggcg gcgagctccg tttgcagcgc ggcgtcgccg acgcccgcga aggccgcggt
     1021 cgcgtcgcct cggtaatact cggcgaagcc gttgaagccc gcgatgccgt actcgatgaa
     1081 cgacagcggc atcggcgtct cgaggttcgc gcgaagttgc gcgacggcgg tcggcacggc
     1141 gtgcaaatag ccgatgaaag cgcgcagccg cgtcgcggcg tcggcgtacg gccgcgcgat
     1201 gtagacgttc ggatcgaggc cgttgtcgaa gtaccaggtc aggttctcgt gcggcgcgtc
     1261 ggcatccgcg agccagaaca gctcgccgcg cgcgacgtgg accagatagt cgcgctcgaa
     1321 gcgctgtgct tcggagagct gcgccgcgtc gaacgcccgc gcatcggcga tcgcttggcg
     1381 aaagagctcg atctgccgcg cgaggccggc ttcgctccag tcgccgatct ggccgtcgag
     1441 ctcgtgccgg ccctggtaca cggcgtagct cggattgagc cggaaatagc cctcgatgta
     1501 ctcgtcgcgg aacgcgtccc aaagagcggt ttgcgacgcg ttcgtcggcg cggcgctcgg
     1561 cggctcgggc ggcgactggc aggcggcgag cacggccgcg agcgcggcgc aggcgaagga
     1621 cgaggacttc atgcggactc ccaaggtcga gcgcgaaaca aagatcacag cacgagggcg
     1681 gggtttgcgt aaactgctca ccgatgaccg ccgcgccgaa agcgatcccc gccttgagcc
     1741 gcacgctgcg ctctcggcac gtggcgctga tctcgctcgg cggcatcatc ggcgccgggc
     1801 tcttcgtcgg cagcagcgcc aacatcgcgt cggtcggccc ggccgtcctc gtaagctacc
     1861 tcgtcgccgg cgcgttgatc ttcctcgtga tgcgcatgct cgccgagatg gcggcggcga
     1921 atccggaggc gctgtcgttc gccgatcacg cgcgcgccgg tctcggctcg tgggccggct
     1981 tcgtctgcgg ttggctctac tggtacttct gggtcgtcgt cgtcggcatc gaggcgatcg
     2041 cgggagccac gatcctcgag aactggatac cgctgccggt ctggcagatc gggctcggcc
     2101 tgatggtgat cctgactgcg acgaacctgt tgtcgacgcg ctcgtacggc gagttcgagt
     2161 tctggttcgc gtcgatcaag gtggcggcga tcctggcgtt catcgccgtc gcgggcagct
     2221 acgcgctcgg cctcacgtcg ccggccggcg cgacgctcgg caacctcgtc gaccacggcg
     2281 ggtttgcgcc gttcggcgca gccgccgtgc tcgccggagt cacgggcatc gtgttctcga
     2341 tgacgggcgc cgagatcgtc accgtcgtcg cggcggagtc gtccgagccg tcgcacgcga
     2401 tcgcggcgat gacgacgtcg ttgatcgcgc ggatcttgct gttctacgtc ggctccgtgt
     2461 tctgcatcct ggccgtcgtt ccgtggaacg aggtcgtgcc cggctcgtcg ccgttcgcgc
     2521 agacgctctt ggtcatcgga atcccgttcg ccgcgacgat catgaacctg atcgtgctcg
     2581 tcgcggtgct gtcgtgcctg aactcgggcc tctacatcac gtcgcgcatg ctgttcgggc
     2641 tcgccgcaca aggcgacgcg ccgcgcgcgc tcgtcgcgct cgacaagcgc ctcgtcccgt
     2701 cgcgagcgat cctgatcggc accgtgttcg gctacctcgc cgtgctcgca tcggtcgtgt
     2761 cgccgcagcg gctgttcacg ttcctcgtca acgcgtcggg cgcgatcatg ctcgtcattt
     2821 atctcttgat cgcgctcgcg cagatccgcc ggcggcatgc gctcgagcag ggcggcgaga
     2881 agcgtctcgt gatccgcatg tggctgcatc cgtggttgag cttaggcacc gtgctctcga
     2941 tcgtgctcgt gctcgcgtcg atgctgttcc ggccggccga gcgcgcgcag ctcgcggcga
     3001 gcgcgctgtc ggtcgccgtc gtgctcgcgg cctacgcttg gaagcggctc gcgcgacgct
     3061 agcgcctccc gaagttgtga tgagggtcgt ggacgctgcc cgacgcgtgg cggacgctgc
     3121 gcggcatcgc cctcgtacga ggatgccgcc gacatgcgtt ccca
//
LOCUS       DASSNN010000223         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1558977, whole genome shotgun sequence.
ACCESSION   DASSNN010000223 DASSNN010000000
VERSION     DASSNN010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558977"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="VFL84_04080"
     CDS             complement(<1..1437)
                     /locus_tag="VFL84_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607831.1"
                     /translation="METQTRSVAVEYRRSDALPKLGWIATLDPRTNRLRVAHGSAVEC
                     RPDWMVEGVWDGRFADGNFHRGAHLFGSGIRRDGEAIYFMPSCALVDRLIYCRNGEEL
                     VVSNSLLVMLAVTGAELDPRHDYLIEGKAVSVGVDDYETSFHVRHASIREFHQVFHKA
                     LVVRGAEIGFERTIGVRAFDDFADYYGTLRGRLAALCQNAADPSRRAPLDLYGTLSNG
                     YDSTAVTTIVRELGVSEFFTYVGSWAASSAGQAKHETAPISAALQVKTVPLRAPREPD
                     IEDELLLRAAIPLAVQVPLLAMARYVEQHSDVAAVFTGFHGDIIWDLNVPEEFVSQAI
                     IRHDMSGLDLSELRLKSGFFNVAVPFFFAASIEDVVAVSRSGEMEPWRVNKRYDRPIS
                     RRIVETAGVPREAFGFLKAGIFTVAIRPTHPELRVKFFDHVGKNIVPLPLLYLRIGCD
                     RYTLRLLEKATHVAKFKLNALKLEAALLR"
     gene            complement(1526..2413)
                     /locus_tag="VFL84_04085"
     CDS             complement(1526..2413)
                     /locus_tag="VFL84_04085"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7607832.1"
                     /translation="MISEAKQAVKIGVHRVLQPLETSLREPAAGALEHVPVFIVGPPR
                     TGSTLLYQLLVRRYRFCYISNLLNRFSETPLALAKLSKAFGGFDAADDFSSRYGDTVG
                     WCAPSQGRECWARWLPESPNALAPDAVANEAKEHVRSTISALQRICGRPFVNKWQPNS
                     VRVRLLSDIFPQALFVRISRSTESTVRSILRGREELCRRGSGWFSVKPPGYRRILNER
                     RPEEQAAWQIAAIEHALDVAGETVGADRFVHVRYEQLTRRPRQVLDRIAAFYEGATRI
                     ELERRCEVPEAFRPHSAPR"
BASE COUNT          444 a          853 c          811 g          401 t
ORIGIN      
        1 acgcaggagc gccgcttcga gcttcagcgc gttcaacttg aacttcgcca cgtgcgtcgc
       61 cttctcgagc agccgcagcg tgtagcggtc gcagccgatg cgcaggtaca gcagcggcaa
      121 cggcacgatg ttcttgccga cgtgatcgaa gaacttgacg cgcagctccg ggtgcgtcgg
      181 gcgaatcgcg accgtgaaga tgcctgcctt caggaagccg aacgcctcgc gcggcacgcc
      241 ggccgtttcg acgatgcggc ggctgatcgg ccggtcgtag cgcttgttga cgcgccacgg
      301 ctccatctcg cccgagcgcg acacggcgac gacgtcttcg atgctcgcag cgaagaagaa
      361 cggcaccgcc acgttgaaga agccgctctt gagccgcagc tcgctcaagt cgagcccgga
      421 catgtcgtgc cgaatgatcg cttgcgagac gaattcctcg ggaacgttca agtcccagat
      481 gatgtcgccg tgaaatcccg tgaacacggc ggcgacgtcc gaatgctgtt cgacgtaccg
      541 tgccatcgcg agcagcggaa cctgcaccgc gagcggaatc gcggctcgca gcagcagctc
      601 gtcctcgatg tcgggctcgc gcggcgcccg cagcggcacc gtcttgacct gaagcgcggc
      661 cgagatcggc gccgtctcat gcttcgcctg accggccgag ctcgcggccc aagagccgac
      721 gtaggtgaag aactcggaga cgccgagctc gcgcacgatc gtcgtgaccg cggtcgagtc
      781 gtagccgttc gagagcgtgc cgtaaaggtc gagcggcgca cgccggctcg gatcggcggc
      841 attttggcag agcgccgcga gccggccgcg aagcgttccg taatagtccg cgaaatcgtc
      901 gaacgcgcgc acgccgatcg tgcgctcgaa cccgatctcg gcgccgcgca cgacgagcgc
      961 tttgtgaaac acctgatgga attcccgaat cgaagcgtgc cgcacatgaa acgacgtctc
     1021 gtagtcgtcg acgccgacgc tgaccgcttt gccctcgatc aaataatcgt gacgcggatc
     1081 gagctccgcg ccggtcacgg caagcatcac gagcaagctg ttcgagacga ccagctcctc
     1141 cccgttgcgg cagtagatca gccggtcgac caacgcgcag gacggcatga agtagatcgc
     1201 ctcaccgtcg cgccggatgc cgctgccgaa caaatgggca ccgcggtgaa aattcccgtc
     1261 cgcgaagcgg ccgtcccaga cgccctcgac catccaatcg ggacggcact cgacggcgct
     1321 tccgtgggcc acgcgcagcc ggttcgtgcg cgggtcgagc gtcgcgatcc aaccgagctt
     1381 cggcaacgca tcgctgcgcc gatattcgac ggcaacggac cgggtttgcg tttccattcg
     1441 cgtgccttct ccgcgtcctt gcgggagccg caggctctcc aagaagcatg ccagtgggct
     1501 cggaatcgcc gcgccttcgc gcgagctacc gcggcgcgct gtgaggacgg aacgcttccg
     1561 ggacctcgca gcgcctttcg agctcgattc tcgtcgcgcc ctcgtagaac gccgcgatcc
     1621 tgtcgaggac ctgcctcggc cggcgcgtca actgctcgta gcgcacgtgg acgaaccgat
     1681 cagcgcccac ggtctcgccg gcgacgtcga gcgcatgctc gatcgcggcg atctgccacg
     1741 ccgcctgctc ttcgggacga cgctcgttca ggattcgccg ataccccggc ggcttcaccg
     1801 agaaccaccc ggagccgcgg cggcacagct cctcgcgtcc tcgcaatatc gagcgcacgg
     1861 tcgactcggt cgagcgtgag attcgaacga acagcgcttg cggaaagatg tcgctcagca
     1921 agcggaccct cacgctgttg ggttgccatt tgttgacgaa cggtctgccg cagatgcgct
     1981 gcagagcaga gatcgtgcta cggacgtgct ccttggcttc gttcgccacc gcatcgggcg
     2041 cgagcgcgtt cggcgactcc ggcagccagc gggcccagca ctcgcgcccc tggctcggcg
     2101 cgcaccagcc gaccgtgtcg ccgtaccggc tgctgaagtc gtcggcggcg tcgaaaccgc
     2161 cgaacgcctt gctgagcttc gcgagcgcga gcggcgtctc ggaaaagcgg ttcagcaagt
     2221 tcgagatgta acaaaagcgg tagcgccgca cgaggagctg atagagcagc gtgctgcccg
     2281 tgcgcggcgg gccgacgatg aacacgggca cgtgctcgag cgcgccggcg gccggttcgc
     2341 gaagactcgt ctcgagcggc tgcaacacgc ggtgcacgcc gatcttgacg gcctgttttg
     2401 cctccgaaat cacacgtccg aagtgcaaca accatgcctc gcgccccgcg aggttgccgc
     2461 gttatgaaaa atcggcgctg gagcgcggca atcgccgcgc cgcttcgcg
//
LOCUS       DASSNN010000224         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1664756, whole genome shotgun sequence.
ACCESSION   DASSNN010000224 DASSNN010000000
VERSION     DASSNN010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664756"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VFL84_04090"
     CDS             complement(<1..686)
                     /locus_tag="VFL84_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7607833.1"
                     /translation="MRSGLPIDEALAPLAAALRAGGNAVVVAPPGAGKSTVVPLALLE
                     EPWARDKRIVMLEPRRLATRAVAERMASLLSERVGETIGYRMRLETRVGKRTRVEVVT
                     EGVFTRMLQSDPALEGVAAVLFDEYHERSLHADVGLAFALDTQRHLAPELRVLAMSAT
                     IDGARVASLLGGAPVIESSGRMFPVAVHYVGRGLPALPGGAEPIDSSVARAVQRALAD
                     SEGDVLVFLPG"
     gene            complement(705..1577)
                     /locus_tag="VFL84_04095"
     CDS             complement(705..1577)
                     /locus_tag="VFL84_04095"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET7607834.1"
                     /translation="MILRSPLFVPGNKANMLEKALTVAPDALVPDMEDSVPAAEKANA
                     RETTAMFLPRLAASGRPVIPRVNALETEWIEADLTAVVGPHVFGISVGKVRRAGDIAA
                     VSALIGKLERRAGLKVGTVRLVPWIETAEAIVNVAEICRASERIVAVAFGGEDLTNDL
                     GVERLEDESQVGYARQALCVAARAAHVLALDTPYFKLRNADGLRDNALKAKSIGFKGK
                     FAIHPEQIETLNECFSPSAQEVAHAERVVAAFEEAERRGRASTSLDGWVIDVPVVKRA
                     RALLELARRARAER"
     gene            1744..2685
                     /locus_tag="VFL84_04100"
     CDS             1744..2685
                     /locus_tag="VFL84_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607835.1"
                     /translation="MALAGLLILAGCGGGGDAPSPAPLAAITKTNALTIASSVVTASL
                     QGGNLGSFVGFASASSPKPERVYYAKVSEIQNAEIGALVRQTRIGAVIPVGPEVTQCT
                     GGGTITLSGDIANPATLSPNDTITFEYAACVEGDTTMNGRFSMRVTGFSGDPASGVFS
                     FAVATTITSLQVTVAGETATLNGSVSISISAAATTTVTTTVSSSSITVGDGGADHTLR
                     DYSSVHTLASGSFTLDVSGSLTSSDFAGMVSFDTTAVLQGMDDGYAFTGQLLITGANG
                     ATIEVIVLDGTFVRLEVDSNGDGIVDATLDVTWDDLT"
BASE COUNT          446 a          960 c          927 g          420 t
ORIGIN      
        1 ccgggcagga acacgagcac gtcgccctcg ctatccgcga gcgcgcgctg caccgcgcgc
       61 gcgacgctcg agtcgatcgg ctcggcgccg ccgggcagcg ccggcaagcc cctgccgacg
      121 taatgcaccg ccaccgggaa catgcgcccc gagctttcga tgacgggcgc gccgccgagc
      181 aagctcgcga cgcgcgcgcc gtcgatcgtc gccgacatcg cgagcacgcg cagctccggc
      241 gcgagatgac gctgcgtgtc gagcgcaaac gcgaggccga cgtcggcgtg caggctgcgc
      301 tcgtgatact cgtcgaacaa cacggcggcg acgccctcga gcgcgggatc gctttgcagc
      361 atgcgcgtga acacgccttc cgtgacgacc tcgacgcgcg tgcgcttgcc gacgcgcgtc
      421 tcgagccgca tgcgatagcc gatcgtctcg ccgacgcgct cgctcaagag agaggccatg
      481 cgctccgcga cggcgcgcgt cgcaagcctg cgcggctcga gcatgacgat gcgtttgtcg
      541 cgcgcccacg gctcctcgag caacgcgagc ggcacgacgg tgctcttgcc ggcgccgggc
      601 ggcgcgacga cgacggcgtt gccgccggcg cgcagcgcgg ccgcgagtgg agccaaggcc
      661 tcgtcgatcg gcagcccgga gcgcatgcgc tgcgctgcgt cgcgctagcg ctcggcgcgc
      721 gcgcggcgcg cgagctcgag cagcgcgcgg gcgcgtttga cgacgggcac gtcgatgacc
      781 cagccgtcga gcgacgtcga ggcgcggccg cgccgctcgg cttcctcgaa cgccgcgacg
      841 acgcgctcgg cgtgcgcgac ctcttgcgcc gaaggcgaga agcactcgtt cagcgtctcg
      901 atctgctcgg gatggatcgc gaacttgccc ttgaagccga tgctcttcgc tttcaacgcg
      961 ttgtcgcgca gcccgtcggc gttgcggagc ttgaagtagg gcgtatcgag cgccagcacg
     1021 tgcgcggcgc gcgcagccac gcacaaagcc tggcgcgcgt agccgacctg cgattcgtcc
     1081 tcgagacgct cgacacccaa gtcgttcgtg agatcctctc cgccgaacgc gacggcgacg
     1141 atgcgctcgc tcgcgcggca gatctcggcg acgttgacga tcgcctcggc cgtctcgatc
     1201 cacggcacga gccgcaccgt gccgaccttc aagccggcgc ggcgctcgag cttgccgatc
     1261 agcgccgaga ccgcggcgat gtcgccggcg cggcgcacct tgccgaccga gataccgaac
     1321 acgtgcgggc cgacgacggc tgtaagatcg gcctcgatcc attccgtctc gagcgcgttg
     1381 acgcgcggga tcacgggcct tcccgaagcc gcgaggcgcg gcaggaacat ggccgtcgtc
     1441 tcgcgagcgt tcgctttctc ggccgccggc acggagtcct ccatgtcggg cacgagcgcg
     1501 tcgggcgcta ctgtcagcgc cttctcgagc atgttcgctt tgttgcccgg aacgaacagc
     1561 gggcttcgaa gtatcacgtc gcagagatta gcactgcggc gctgaaacag cgccgacgcc
     1621 gaggcgggaa tgaagtgtga gccgattccg cgccgccgtc cgccgcactg tctacgctgc
     1681 gcaacgatcc tgcacgcagg cggaaatggg ggtgtgatta tgaacaatcg aagcacatgg
     1741 tccgtggcct tagcaggtct cctgatcttg gcgggctgtg gcggaggcgg cgatgcacct
     1801 tcgccggcgc cgctcgccgc gatcacgaag acgaatgctc tcacgatcgc gagctccgtc
     1861 gtcaccgcct ccctccaagg cggcaacctg ggatcgttcg tcggcttcgc gtctgcgagc
     1921 tcgccgaaac ccgaacgcgt ctattacgcg aaggtgtccg agatacaaaa cgcggagatc
     1981 ggcgcgctgg tgcggcagac gcgaattggc gccgtgattc cagtcggccc cgaggtgacc
     2041 caatgcaccg gcggcggcac gatcacactc tcgggcgaca tcgccaatcc cgccacgctg
     2101 agcccgaacg acacaatcac cttcgaatac gctgcgtgcg tcgaaggcga cacgacgatg
     2161 aacggccgct tctcgatgcg cgtcacgggc ttctccggcg accctgcgag cggcgtgttc
     2221 tcattcgccg tggccaccac gatcacgtcc ttgcaagtca cggttgccgg cgagacggcg
     2281 acgctgaacg gctccgtctc catctcgatc agcgcggccg ctactacgac ggtgaccacg
     2341 accgtgagct cgagctcgat cacggtaggc gatggcggtg ccgaccacac gctgcgcgac
     2401 tactcctctg ttcacacgct tgcgtcgggg tccttcacgc tcgacgtgag cggcagcctc
     2461 acgagctctg atttcgcggg catggtgagc ttcgacacga cggcggtgct gcaaggcatg
     2521 gacgacggct acgccttcac gggccaattg ctgatcacgg gcgcgaacgg cgcgacgatc
     2581 gaggtcatcg tgctcgacgg cacgttcgtg cggctcgaag tcgacagcaa cggcgacggc
     2641 atcgtcgatg cgacgctcga cgttacctgg gacgatctga cgtaggcgga tcggccgagg
     2701 ctctctcggc tacgcgagcg tcggagtcga ctcgggcaca gcaacgcgcc cgg
//
LOCUS       DASSNN010000225         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_654406, whole genome shotgun sequence.
ACCESSION   DASSNN010000225 DASSNN010000000
VERSION     DASSNN010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654406"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VFL84_04105"
     CDS             complement(<1..1271)
                     /locus_tag="VFL84_04105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7607836.1"
                     /translation="MAAILSQGARPGGAVSGVRRLARDWWALALVVAVGGYLRLAHLG
                     ALGFRWDEDLSGLAVRAILEKGVPELPSGMIYLRGGLFSHLMAASASLFGFSEFALRL
                     PAALFGIALILAAYLFGTSLFGRLVGLLTAALVAVSFWDIDLARYARFYSAFSVLYVL
                     TLYAIWRFRVKAPSAAGGVLCVVLAVVTLSLHDLAYSLALAFFYPLVLRGSAAWRSPR
                     EWVWPVAASAVLAAFYLGWRALVRASRELPALAGDSAAGAGREVDELTRSFVALPDMP
                     LLTGVLERAPLFVPLLVLLAVGAALNLTRRFGSRTHERVWLALIAACAAVQLFNLALI
                     GTLLLAFSKRTGLPSLRSAEVLAAVALIGAAFVAWVVAVLALGVDVSPLEPLALHTVV
                     RELLDFPYFYVFWGFPNEWPLATAVATIGALV"
     gene            1497..2462
                     /locus_tag="VFL84_04110"
     CDS             1497..2462
                     /locus_tag="VFL84_04110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7607837.1"
                     /translation="MTEVSIVVPLYNEQDNVARLCAATHEALLEMRRTYELVLVDDGS
                     TDDTVAAASAIARSDARVRLVVLACNSGQTAAMAAGMAHARGRYLVTMDGDLQNDPRD
                     IPLLVDTLEKDYDLVAGWRMKRQDKLVTRKIPSRVANWLIGKITGIPIRDNGCSLKAY
                     RASVMRHVPLYSEMHRFIPAMSSIAGARIVQVPVRHHARQFGQSKYGLSRIYKVLLDL
                     IAIRLVVAYANRPLVWFLKMAVGPAVLAAAFTAGGIVRALGGEYSLPLFGSGLILALA
                     VGFLLALGSLGELAKHCARGDVVPLVTASAKYSKPIPATNSKDQP"
BASE COUNT          494 a          839 c          852 g          356 t
ORIGIN      
        1 accagcgcgc cgatcgtcgc cacggcggtc gcgagcggcc attcgttcgg aaaaccccag
       61 aagacataga agtacgggaa atcgagcagc tcgcggacga cggtatgcag cgcgagcggc
      121 tcgagcgggc tcacgtccac accgagcgca agcaccgcga cgacccaggc gacgaaggcg
      181 gcgccgatca acgcgacggc agcgagcact tcggcgctgc gcagcgaagg gaggcccgtg
      241 cgcttcgaga atgcgagcag cagcgtgccg atcagcgcga gattgaagag ctgcaccgcc
      301 gcgcacgcgg cgatcaaggc gagccacact cgctcgtgcg tgcggctgcc gaagcggcgc
      361 gtcaagttga gcgccgcgcc taccgcgagc agcacgagca gcggaacgaa gagcggagcg
      421 cgctcgagca cgcccgtgag gagcggcatg tccgggagcg cgacgaacga gcgcgtgagc
      481 tcgtccacct ctcggccggc ccccgccgcc gagtcgcccg caagcgccgg cagctcgcgc
      541 gaggcgcgga ccaacgcgcg ccagccgagg tagaacgccg cgagcacggc gctcgccgcg
      601 acgggccaga cccactcgcg cggcgatcgc catgcggccg agccgcgcag cacgagcgga
      661 tagaagaacg cgagcgcgag cgagtaggcg aggtcgtgca aggacagcgt gacgactgcg
      721 agcacgacgc acaaaacgcc gcccgccgcg ctcggcgcct tgacgcggaa ccgccagatc
      781 gcgtacagcg tcagcacgta aagcacgctg aaggcggaat agaagcgcgc gtagcgggcg
      841 aggtcgatgt cccaaaagga caccgcgacc aacgccgcgg tcaagagacc gacgagccgg
      901 ccgaagagtg acgtgccaaa caagtaagcg gcgaggatca gcgcgatgcc gaacagcgcg
      961 gccgggagcc gcagcgcaaa ttcgctgaag ccgaacagcg aagcgctggc cgccatgaga
     1021 tggctgaaca atccgccgcg tagatagatc attccgctcg gcagctccgg cacgcctttt
     1081 tcgaggatcg cgcgcacggc gagcccggag agatcctcgt cccaacggaa cccgagggcg
     1141 cccaagtgag cgagccgcag gtagcccccg acggcgacga cgagggcgag cgcccaccaa
     1201 tcgcgagcga gacgccgaac accgctgacg gcgccaccgg gtcgagctcc ctgactcaat
     1261 attgcagcca tcaccccagg caataccggc ccctccggcc ggtgtgcgtt gcttggcatt
     1321 gcctttgcaa gatccaggcc gccggtggcg agagcggatg cgctcaaacg acggcgtgat
     1381 gcccgcgcga ggcggtcatt ggcgccgacg cgacgttttt cgccaagggc agggaggttt
     1441 tcgtttttgc aatacgagat ttgcaaacgg cgcacgagcg caagcgcagt cgcgcgatga
     1501 ccgaagtctc gattgtcgtt ccgctctaca acgagcaaga caacgtcgca cggctttgcg
     1561 ccgcgacgca cgaagcgctc ttggaaatgc gccgaaccta cgagctcgtg ctcgtcgacg
     1621 acggcagcac ggacgacacg gttgccgcgg ccagcgccat cgcgcgcagc gacgcgcggg
     1681 tgcggctcgt ggtgctcgcc tgcaattcgg ggcagaccgc cgcgatggcg gctggaatgg
     1741 cgcacgcgcg cgggcgctat ctcgtcacga tggacggcga cctgcagaac gatccgcgcg
     1801 acattccgct gctcgtcgat accctcgaga aagactacga cctcgtcgcc ggatggcgca
     1861 tgaagcgcca agacaaactc gtcacgcgca agattccatc gcgcgtcgcg aattggctga
     1921 tcggcaagat caccggaatt ccgattcgcg acaacggctg ctcgttgaag gcgtaccgcg
     1981 cgtccgtcat gcggcacgtg ccgttgtact cggagatgca tcgcttcatt ccggcgatga
     2041 gctccatcgc gggtgcgcgc attgttcaag tgcccgtgcg ccaccacgcg cggcagttcg
     2101 gtcaatcgaa atacgggctg tcgcgcatct acaaagtgtt gctcgacttg atcgcgatcc
     2161 ggctcgtggt cgcctacgcg aaccgaccgc tcgtctggtt cctcaagatg gccgtcgggc
     2221 cggccgtact tgccgccgca ttcacggcgg gcggcatcgt gcgcgcccta ggcggggagt
     2281 attcgctgcc gttgttcggc tcggggctca ttctggctct cgccgttgga tttcttcttg
     2341 cgcttggctc gctcggtgag ctggcgaagc attgcgcgcg cggtgacgtg gtgccgctcg
     2401 tgaccgcttc agccaagtat tcgaagccca ttcctgcaac gaattcgaag gatcagccat
     2461 gatcgacatc gatgtgatcg tgcccgtcgc ggaccggacc gacgatctga ccgcgctgca
     2521 tcgccggcgc agtgaagcgc t
//
LOCUS       DASSNN010000226         3414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1664949, whole genome shotgun sequence.
ACCESSION   DASSNN010000226 DASSNN010000000
VERSION     DASSNN010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664949"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFL84_04115"
     CDS             <1..571
                     /locus_tag="VFL84_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7607838.1"
                     /translation="KDLDFEQRLIVVRSGKGGKDRVTVLPNSVVAALEVHLMRRREQH
                     QQRLARGGGEVYLPYALERKYPNAGRSWPWQFVFAANMDVFLHELGRPASWHLHEKSV
                     QRAVQSALRRAGIEKPASCHTLRHSFATHLLQRGQDIRTIQELLGHKDVSTTMIYTHV
                     AGVGATGTVSPLDAGAGPAAPQRPKPSGA"
     gene            complement(580..2412)
                     /gene="glmS"
                     /locus_tag="VFL84_04120"
     CDS             complement(580..2412)
                     /gene="glmS"
                     /locus_tag="VFL84_04120"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET7607839.1"
                     /translation="MCGIVGAIAERNIVPVLVEGLKRLEYRGYDSAGLAVVQGDQKLG
                     LCRTVGKVSQLESKLSAKPMAGRLGVAHTRWATHGGVTEANAHPHLSGGRVAVIHNGI
                     IENYQPLKDELIAKGYEFNSETDTEVAAHLIHEYLKQGHELLEAMRLAVARFVGAYAL
                     LVFDAKNPERIVVTRVASPLVIGLGLGENFVASGVPALLPVTQRFIYLEQGDIAEITR
                     DAVRIFDAKGELVARQVHETQWSQEAAEKGPYRHFMLKEIFEQPSALADTLYGRVVNK
                     RVVPESLGPRAAELLPKIENVHIVACGTSYHAGSVGRYWFESIAGVPCQVEIASEYRY
                     RGVVVPPNTLFLTLSQSGETADTLEALRMAKTAGYLASLTICNSPHSSMVRESDLVMM
                     TQAGPEIGVASTKAFTTQLLSLMIVTLMLGRHRGLTAAREAEFVPHLYHAAAAVEQAL
                     GMNDLIRLLAEDFAEKEHALFLGRGPMWPIAMEGALKLKEISYIHAEAYAAGELKHGP
                     LALIDEAMPVIVVAPNNDLLDKLKSNMQVVRARGGQLYVFADRETGLESEPGVRVIPM
                     PHADRVIAPIVYTVPLQLLAYHAAVLKGTDVDQPRNLAKSVTVE"
     gene            complement(2453..>3414)
                     /gene="glmU"
                     /locus_tag="VFL84_04125"
     CDS             complement(2453..>3414)
                     /gene="glmU"
                     /locus_tag="VFL84_04125"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041718097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET7607840.1"
                     /translation="GRVVRDSRGEVARIVEHNDATDDERSVNEINTGLIACDASALRR
                     YLGDLKSNNAQREYYLTDVIGMAVAERVPVDGIVAESPTEVLGINDRAQLAAAERAWQ
                     RQQAADLMARGATLADPERIDIRGEVVVGRDVFIDVGVVLQGKVVLGDRAHIGPYAVI
                     TDCTLGADTVVHPHTVMNGSAAGDDCEIGPFSRLRPGTVLAGHVKVGNFVEMKNSHIA
                     PHSKVNHLTYVGDATVGTRVNVGAGTITCNYDGANKHRTVIGDNAFIGSGVMLVAPVE
                     VGAGATIGAGSTITKDTPAEELTLARSKQVTIPGWKRPQKKPK"
BASE COUNT          548 a         1122 c         1143 g          601 t
ORIGIN      
        1 caaggatctc gacttcgagc aacgactgat cgtcgtccgc agcggcaagg gcggcaaaga
       61 tcgcgttacg gtgttgccga actcggtcgt cgccgcgttg gaagtacatc tgatgcggcg
      121 gcgcgagcag catcagcaac gactcgcccg cggcggcggc gaggtctatt tgccgtacgc
      181 gctcgagcgc aagtacccca acgcaggccg tagctggcct tggcaattcg tgttcgccgc
      241 gaatatggat gtattcctgc acgagctggg gcgaccggca agctggcatt tgcacgaaaa
      301 atcggtccaa cgcgccgtgc agagcgcgtt gcggcgcgcg ggcatcgaga aacctgcgag
      361 ctgccacaca ttgaggcaca gttttgcaac gcacctgctg caacgcgggc aggacatccg
      421 cacgatccaa gagctcctcg gccacaagga cgtcagcaca acaatgatct acacgcacgt
      481 agcgggcgtc ggtgcaacgg gcaccgtcag cccgctcgac gcgggagccg ggccagctgc
      541 gccgcaaagg ccgaagccga gtggcgcctg aaagcggcgt tattccaccg tcaccgactt
      601 cgcgagattc cgcggctgat cgacgtccgt acccttcaac accgcggcgt gataggcgag
      661 cagctgcaac ggcacggtgt agacgatcgg cgcgatgacg cggtcggcgt gcggcatcgg
      721 gatcacgcgc acgccgggct ccgattcgag gccggtctcg cgatccgcaa acacgtacag
      781 ctggccgccg cgcgcgcgca ccacctgcat gttggacttg agcttgtcga gtagatcgtt
      841 gttcggcgcg acgacgatga ccggcatcgc ttcgtcgatc agcgcgagcg ggccgtgctt
      901 gagctcgccg gcggcgtagg cctcggcgtg gatgtacgag atctccttga gcttgagcgc
      961 gccttccatc gcgatcggcc acatcggtcc gcgccccaga aacagcgcgt gctctttctc
     1021 ggcgaaatcc tcggcgagca ggcggatcaa atcgttcatg ccgagcgctt gctcgacggc
     1081 ggccgcggcg tgataaagat gcggcacgaa ctcggcctcg cgcgcggcgg tgagcccgcg
     1141 atgccggccc agcatcaacg tcacgatcat cagcgagagc agctgcgtcg taaacgcctt
     1201 cgtgctcgcg acgccgatct cgggtccggc ctgcgtcatc atgacgagat cggattcgcg
     1261 caccatcgag ctgtgcgggc tgttgcagat cgtcagcgac gcgagatagc ccgcggtctt
     1321 cgccatgcgc agcgcttcga gcgtgtcggc cgtctcgccc gactgcgaca gcgtcaagaa
     1381 caacgtgttc ggcggcacga cgacgccgcg gtagcgatac tcgctcgcga tctcgacttg
     1441 acacggaacg ccggcgattg actcgaacca atagcgcccg acgctgccgg cgtgatagct
     1501 cgtgccgcac gcgacgatgt ggacgttctc gatttttggc agcagctccg cggcgcgcgg
     1561 cccgagcgat tccggcacga cgcgcttgtt gacgacgcgg ccgtacagcg tgtcggcgag
     1621 cgcgctcggc tgctcgaaga tctccttgag catgaagtgc cggtacgggc ccttctccgc
     1681 cgcttcctgg ctccactgcg tctcgtgcac ctggcgcgcg acgagctcgc ccttcgcgtc
     1741 gaagatgcgc acggcgtcgc gcgtgatctc ggcgatgtcg ccttgctcga gatagatgaa
     1801 ccgctgcgtg acgggcaaga gcgccggcac gccgctcgcg acgaagttct cgcctaagcc
     1861 taagccgatg acgagcgggc tcgcgacgcg tgtgacgacg atgcgctccg gattcttggc
     1921 gtcgaacacg agcagcgcgt aggcgccgac gaagcgcgcg acggcgaggc gcatcgcctc
     1981 gagcagctcg tggccctgtt tcaagtactc gtggatcagg tgcgccgcga cctccgtgtc
     2041 ggtctcggaa ttgaactcgt agcccttcgc aatgagctcg tctttgagcg gctgatagtt
     2101 ctcgatgatg ccgttgtgga tgacggcgac gcgcccgccc gacagatgcg gatgcgcgtt
     2161 ggcttcggtg acgccgccgt gcgtggccca gcgcgtgtgc gcgacgccga gccgcccggc
     2221 catcggcttc gcggaaagct tgctctcgag ctgcgacacc ttgccgacgg tgcggcaaag
     2281 cccaagcttc tggtcgcctt gcacgacggc gagaccggcc gagtcgtagc cgcggtactc
     2341 gagccgcttc aggccctcga cgagcacggg gacgatattg cgctctgcaa tggcgccgac
     2401 gatgccgcac atacggttta agtccttact ctgcgttcgc gagcgaacgc gttcacttgg
     2461 gtttcttctg cggccgtttc cagcccggaa tcgtgacttg cttcgaccgc gcgagcgtca
     2521 gctcctcggc cggcgtgtcc ttcgtgatcg tcgagcccgc gccgatcgtc gcgccggcgc
     2581 cgacctcgac gggcgcgacg agcatgacgc cggagccgat gaacgcgttg tcgccgatca
     2641 cggtgcggtg cttgttcgcg ccgtcgtaat tgcaggtgat cgtgccggcg ccgacgttga
     2701 cgcgagtgcc gaccgtcgcg tcgccgacgt acgtcaagtg gttcactttg ctgtgcggcg
     2761 caatgtggct gttcttcatc tcgacgaagt tgccgacctt gacgtggccc gcgagcacgg
     2821 tgccgggccg caggcgcgag aacggcccga tctcgcagtc gtcgccggcg gcgctgccgt
     2881 tcatgacggt gtgcgggtgc acgacggtat cggcgccgag cgtgcagtcg gtgatgacgg
     2941 cgtacggccc gatgtgcgcg cgatcgccta gcacgacctt gccttgcagc acgacgccga
     3001 cgtcgatgaa gacgtcgcgg ccgacgacga cttcgccgcg aatgtcgatg cgctcggggt
     3061 ccgcgagcgt cgcgccgcgc gccatgagat cggcggcttg ttgccgctgc cacgcgcgct
     3121 ctgcggccgc gagctgcgcg cgatcgttga tgccgagcac ctcggtcggc gactcggcga
     3181 cgatgccgtc gacgggcact cgctccgcga ctgccatgcc aatgacgtcg gtgaggtagt
     3241 actcgcgctg cgcgttgttg ctcttcaagt cgccgagata gcgccgcagc gccgacgcgt
     3301 cgcacgcaat gaggccggtg ttgatctcgt ttacgctgcg ctcgtcgtcc gtcgcgtcgt
     3361 tgtgctcgac gatgcgggcg acttcgccgc gcgagtcacg cacgacgcgc ccgt
//
LOCUS       DASSNN010000227         7048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1176739, whole genome shotgun sequence.
ACCESSION   DASSNN010000227 DASSNN010000000
VERSION     DASSNN010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176739"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFL84_04130"
     CDS             <1..331
                     /locus_tag="VFL84_04130"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET7607841.1"
                     /translation="LFFCVDIAGVEANIQLARYDSSDRGFYNWHTDFAGIRPLRKLSI
                     SIQLSRAEDYEGGDLELMYGAEPQKLDKTRGAFIVFPSFMLHRVTPVTKGTRWSLVAW
                     IAGTRWR"
     gene            complement(357..944)
                     /locus_tag="VFL84_04135"
     CDS             complement(357..944)
                     /locus_tag="VFL84_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607842.1"
                     /translation="MKKAIVAALFSVPFAAHAGKIVVEYSGVVSSVERNWLAAEPPYS
                     VGDAISGTLLIDAGLAPTDELAPDLRIGRYSGGTALDFILGSKHPGGIGSADLALVHD
                     DWQPSGGAPLEDGIVIKDSAIGIDGASSLLLGLQRPISAGQIFSDDSLSQSFVVEREP
                     GTSLWGYLERGFGEFWQLVSFTVDRLSMTPLVCKP"
     gene            1323..3332
                     /locus_tag="VFL84_04140"
     CDS             1323..3332
                     /locus_tag="VFL84_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607843.1"
                     /translation="MKFTLCRGSSIVVCAALVACNGAPTESTSAPGAPEGGDSAARGN
                     ERIVYSSLRPGNWDIFYFPGGGAAPRRLTDHPGLDYDAALSPDGRWVVFTSERRGNPD
                     LYALDVERGGEPRLLIDSAAMEDQVAFSPDGRSIAFVGTASGNAEIYVIPFSPETTQT
                     LAAAANVTNHPGGDFRPDFSPDGSAIAFSTDRDTPVAGHPIFGFTRQREGDVYVMNRD
                     GGNAKRLTATPDWDGSPEWSADGRTIYFYSARPREIVGPPTSPILGQEGGFRIWAMNA
                     DGSNPRVVTPEGVEALAPAVTAGGRVAYQKRTGYAHWSIESVAADGSGARAETDASND
                     YWLPDFAANGAMVVHGVGPAVGETQAVEAILGAGALLAADYPADVAISDRTVTLYPMR
                     HTTGLAPHPDRDEAAVTIENEAGTRFVLADFGGGNQRELFSVPNVGIVSGTPNRIFDI
                     KWSDDGQWITYTQGFFFGQGTDEADIWVMRADGSERRNLSEDTAANEGVAGFSPDAER
                     VVYRSSRNGRFDLYLGTRDGKDVRALTNDEARENFPVFSPDGRSIAFSSNKDGPRDIR
                     GNLTFDNYVLSLAPDGTPGSVQRISDHPGQDSHPWFSPDGAWIVYTSERGGINDEEPM
                     VQEVVFGPQMYGELFARRLRDGFEVRLTHNKWEEGNPFWLRPSSR"
     gene            complement(3312..5747)
                     /gene="ppsA"
                     /locus_tag="VFL84_04145"
     CDS             complement(3312..5747)
                     /gene="ppsA"
                     /locus_tag="VFL84_04145"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HET7607844.1"
                     /translation="MSAAETSVAREYVVPFSRLGIKDVAVVGGKNASLGEMIGALAQA
                     GVSVPCGFATTADAYREFLCQDRLDARIAKALASLDVEDVAALSSTGKQIRDWILRTR
                     FPPALEAAVLLALESMDRERADRRVPLAVAVRSSATAEDLPDASFAGQQETFLNVRGG
                     ADVLNAMHEVFASLFNDRAIAYRVHHGFDHAAVALSVGVQQMVRSDVGASGVMFTLDT
                     DSGFRDVVLITSSYGLGETIVQGAVNPDEFCVYKPSLVAGRQAILRRTLGAKALKMIY
                     AEPGGANRVATVPVSDAERARFSLTDDDVLTLARQALTIEKHYGRPMDIEWGKDGDTG
                     EIFILQARPETVQSRAGRTIRRYTLRERSRALISGRSIGQRIGAGRARVIRDPKEMIR
                     VQHGDVLVTDMTDPDWEPVMKRAAAIVTNRGGRTCHAAIIARELGIPAVVGCGEATER
                     IADGRPVTVSCAEGETGHVYDGALPFECTDVELDSLPEIPVKIMMNVANPERAFDFAG
                     IPNRGVGLARIEFIINRNVGVHPRALLELDQLDGEIKERVRAQMAGYTDPRAFFVDKL
                     AEGVAQIAAAFAPHPVIVRLSDFKSNEYANLIGGRAFEAVEENPMLGFRGAARYVDPA
                     FRPCFELECRALKRVRDEMGLENVELMVPFVRTVEEARAVTALLAENGLARGTRGLRI
                     IMMCEIPTNALLADRYLDHFDGMSIGSNDMTQLTLGVDRDSGVVASSFDERDDAVKAL
                     LSMAIQACKRRGKYIGICGQGPSDHPDFARWLLEQGIDSISLNPDTVLETWLFLAREQ
                     PQAARKPLAATRP"
     gene            5831..6649
                     /locus_tag="VFL84_04150"
     CDS             5831..6649
                     /locus_tag="VFL84_04150"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HET7607845.1"
                     /translation="MRRRTVYFVSDQTGVTSETVGHSVLAQFAGLEVREVTLPFISNV
                     EKAQEAVRRINRTAEEEGARPIVFSTLVEDELRGIVMKCNALFLDCFAGLVIPLEREL
                     ERRSSHRTGRSHPVADAAYTLRMDAINYALANDDGAGGDYGRADIVLVGVSRSGKTPT
                     CLYMALQYGVFAANYPLTDDELEAGVLPRRLEAHRKKLHALTIKPERLRQIREERRPQ
                     SRYASPQQVQYEIRAAEAIFSRYGIAVLDTTESSIEEIASRILSVAGVERRLRP"
BASE COUNT         1169 a         2352 c         2396 g         1131 t
ORIGIN      
        1 cctgttcttc tgcgtcgaca tcgccggcgt cgaagccaac attcagctcg cgcgctacga
       61 cagctccgac cgcggcttct acaactggca cacggacttc gcgggcattc gaccgctgcg
      121 caagttgtcg atctcgatcc agctctcgcg cgcagaggat tacgaaggcg gcgacctcga
      181 gctcatgtac ggcgccgagc cgcagaagct cgacaagacg cgcggcgcat tcatcgtgtt
      241 cccgagcttc atgctgcatc gcgtgacgcc ggtgacgaag ggcacacggt ggagcctcgt
      301 cgcgtggatt gcgggcacgc gctggcgctg agaagcgagg tcgagccgcg cctagctcag
      361 ggcttgcaaa cgagcggcgt catcgacaag cggtcgaccg tgaagctgac gagctgccag
      421 aactctccga agccgcgctc gaggtagccc cagaggctcg tgccgggctc gcgctcgacg
      481 acgaacgatt gcgacagcga atcgtcggag aagatttgcc ccgccgagat cggccgctgc
      541 agcccgagca gcaagctcga tgcgccgtcg attccgatcg cactgtcctt gatgacgatg
      601 ccgtcctcga ggggagctcc gcctgacggc tgccagtcgt cgtggacgag agccaagtct
      661 gccgatccga ttccacccgg gtgcttcgag cccaagatga agtcgagggc cgtgccgccc
      721 gagtagcggc cgatccttag gtccggcgcg agctcgtccg tgggcgcgag gcccgcatcg
      781 atcagcagcg tcccggagat ggcgtcgccg accgaatacg ggggctccgc ggcgagccag
      841 ttccgctcga ccgatgacac gactccgctg tattcgacga cgattttccc ggcatgggcc
      901 gcgaacggga cagagaataa tgccgcgacg atcgccttct tcattcgcga gctacagcca
      961 ctgccacacg atcagaacgt agccgagcat cgccgtggcg atcagtgcga acgggagaag
     1021 gcgatggctc gacaggtcgc gtttgagccc gggatcggtt gcaacctttc gacgaactgt
     1081 tccagccttt gcaatttgca cggtcccccc tttcggacgg ggcccgtaga gcctgcgtcc
     1141 ggcgcaaaaa atgagcgtgg tcgggactag gcactgcaag tttcgtgcaa gaacggcctt
     1201 cggcctgcga accgcgacac gttctggccc tgagagccgg acggcgtttg attggcgggc
     1261 cgcttgcctc gacaatcgcg gctggccggt gcgccggcgc ctataaagaa aggggggaag
     1321 ccatgaaatt cacgctttgc cgcggctcgt cgatcgtcgt ctgcgccgcg ctcgttgcat
     1381 gcaacggcgc accgacggag agcacgagcg cgcccggggc gcccgagggc ggtgattctg
     1441 ccgcgcgcgg caacgaacgc atcgtctatt cgtcgctgcg gcccggcaat tgggacatct
     1501 tctacttccc gggcggcggc gcggcaccgc ggcggctcac cgatcatccg ggcctcgact
     1561 acgacgcggc gctgtcgccc gacgggcgct gggtcgtgtt cacgtcggag cggcgcggca
     1621 accccgatct gtacgctctc gacgtcgagc gcggcggcga gccgcgtctc ttgatcgaca
     1681 gcgcggcaat ggaagaccag gtcgcgttct cgccggacgg ccgcagcatt gcgttcgtcg
     1741 gcacggcgag cggcaacgcc gagatctacg tgatcccgtt cagccccgag acgacgcaga
     1801 cgctcgcggc agccgcgaac gtgacgaatc atcccggcgg cgactttagg cccgacttct
     1861 cgcccgacgg atcggcgatc gcgttctcga cggaccgcga tactccggtg gccggccatc
     1921 cgatcttcgg attcacccgc cagcgcgaag gcgacgtcta cgtcatgaat cgcgatggcg
     1981 gcaacgcgaa gcggctcacg gcgacgcccg actgggacgg ctcgccggag tggtcggcgg
     2041 acggccgcac gatctatttc tattcggcgc ggccgcgaga gatcgtcggt ccgccgacga
     2101 gcccgatcct gggacaagag ggcggcttcc gaatctgggc gatgaatgcc gacggctcga
     2161 acccgcgcgt cgtgacgccg gaaggcgtcg aagcgctggc gccggcggtc accgccggtg
     2221 ggcgcgtcgc gtatcagaag cgcaccggct acgcgcactg gagcatcgag tccgtcgccg
     2281 ccgacgggtc gggcgcgcgc gccgagaccg acgcgtcgaa cgactattgg ctgcccgatt
     2341 tcgcggcgaa cggcgcgatg gtcgtgcacg gtgtcggccc cgccgtcggc gagacgcaag
     2401 ccgtcgaggc gattctcggg gcgggcgcgc tgctcgctgc ggattatccg gccgacgtcg
     2461 cgatctcgga tcgtaccgtc acgctgtatc cgatgcggca cacgacgggt ctcgcgccgc
     2521 acccggaccg cgacgaggcc gccgtcacga tcgagaacga ggcgggcaca cgcttcgtgc
     2581 tcgccgactt cggcggcggc aaccaacgcg agctgttctc cgtgccgaac gtcggcatcg
     2641 tcagcggcac gccgaaccgc atcttcgaca tcaagtggtc cgacgacggc caatggatca
     2701 cgtacaccca gggatttttc ttcggccaag gcaccgatga agccgacatc tgggtcatgc
     2761 gcgcggacgg ctccgagcgc cgcaacctga gcgaagacac cgccgcgaac gaaggcgtcg
     2821 cggggttctc gccggacgcc gagcgggtcg tgtaccgcag ctcgcgcaac ggccgcttcg
     2881 atctctactt gggcacgcgc gacggcaagg atgtcagagc gctgacgaac gacgaggcgc
     2941 gcgagaactt ccccgtgttt tcgccggacg gtaggtcgat cgcgttctcg tcgaataagg
     3001 acggcccgcg cgacattcgc ggcaacctga cgttcgacaa ctacgttctc tcgctcgcgc
     3061 ccgacggcac gccgggcagc gtgcagcgaa tctccgacca tcccggccag gactcgcacc
     3121 cgtggttctc gcccgacggc gcctggatcg tctacacgtc ggagcgcggc ggcatcaacg
     3181 acgaggagcc gatggtccag gaggtcgtct tcggcccgca gatgtacggc gagctgttcg
     3241 cgcggcgcct ccgcgacggc ttcgaagtgc gcctcacgca caacaagtgg gaggaaggga
     3301 atccgttctg gctacggccg agtagccgct agcggcttgc gcgccgcctg cggctgctcg
     3361 cgcgcgagga acagccacgt ctcgagcacg gtgtcggggt tcagcgagat gctgtcgatg
     3421 ccttgctcga ggagccagcg cgcgaagtcg ggatggtccg acggcccttg gccgcagatg
     3481 ccgatgtatt tgccgcgccg cttgcaggcc tggatcgcca tcgatagcag cgccttcacg
     3541 gcgtcgtcgc gctcgtcgaa agagctcgcg acgacgccgg aatcgcggtc gacgccgagc
     3601 gtcagctgcg tcatgtcgtt cgagccgatc gacatgccgt cgaagtgatc gagataacga
     3661 tcggccagca aggcgttggt cgggatctcg cacatcatga tgatcctgag cccacgggtt
     3721 ccgcgcgcga gcccgttctc ggcgagcagc gccgtgacgg cgcgcgcctc ctcgaccgtg
     3781 cgcacgaacg gcaccatgag ctcgacgttc tcgagcccca tctcgtcgcg gaccctcttg
     3841 agcgcgcggc actcgagctc gaagcacggg cggaacgccg gatcgacgta gcgcgcggcg
     3901 ccgcggaacc cgagcatcgg gttctcctcg acggcctcga acgcgcggcc accgatcaga
     3961 ttcgcgtact cgttcgactt gaaatcggag agccgaacga tcacgggatg cggcgcgaac
     4021 gcggccgcga tctgcgcgac gccttcggcg agcttgtcca cgaagaaggc gcgcggatcg
     4081 gtgtagccgg ccatctgggc gcgcacgcgc tccttgattt cgccgtcgag ttggtcgagc
     4141 tcgagcaagg cgcgcggatg cacgccgaca tttcggttga tgatgaactc gatgcgcgcg
     4201 aggccgacgc cgcggttcgg aatcccggcg aaatcgaacg cgcgctccgg gttcgcgacg
     4261 ttcatcatga tcttgacggg aatctcgggc agcgagtcga gctcgacgtc cgtgcactcg
     4321 aacggcaatg cgccgtcgta gacgtgtccc gtctcgcctt cggcgcacga gacggtcacg
     4381 ggtctgccgt cggcgatccg ctccgtggcc tcgccgcagc cgaccacggc cgggatcccg
     4441 agctcgcgcg cgatgatcgc cgcgtggcac gtgcggccgc cgcggttcgt cacgatcgcc
     4501 gcggcgcgct tcatgaccgg ctcccagtcg ggatccgtca tgtcggtcac gagcacgtcg
     4561 ccgtgctgca cgcggatcat ctccttcggg tcgcgaatca cgcgcgcgcg gccggcgccg
     4621 atgcgctggc cgatgctgcg gccggagatc agggcgcgcg agcgctcgcg cagcgtgtag
     4681 cgccggatcg tccggcccgc gcggctctgg acggtctccg ggcgcgcctg aagaatgaag
     4741 atctcgccgg tgtcgccgtc cttgccccat tcgatgtcca tggggcggcc gtagtgcttt
     4801 tcgatcgtta gcgcttggcg cgcgagcgtc aggacgtcgt cgtccgtcag cgagaagcgc
     4861 gcacgctcgg cgtccgaaac cggcacggtg gcgacgcggt tcgcgccgcc cggttccgca
     4921 tagatcattt tcagcgcttt cgcgcccaac gttcgccgca agatcgcctg gcggccggcc
     4981 acgagcgacg gcttgtagac gcagaactcg tcgggattga ccgcgccttg cacgatcgtc
     5041 tcgccgagtc cgtacgacga cgtgatcagc acgacgtcgc ggaagccgga gtccgtgtcg
     5101 agcgtgaaca tgacgccgct cgcgccgacg tcgctgcgca ccatctgctg cacgccgacg
     5161 gacagcgcga cggcggcgtg atcgaacccg tggtgcactc gataggcaat cgcccggtcg
     5221 ttgaacagcg aggcgaagac ttcgtgcatg gcgttgagca cgtccgcgcc gccgcgcacg
     5281 ttcaagaacg tctcctgctg gcccgcgaac gaagcatcgg gcaggtcctc ggccgttgcc
     5341 gacgaacgca cggccacggc gagaggcacg cggcgatccg cgcgctcgcg gtccatgctc
     5401 tcgagcgcca agagcacggc cgcttcgagc gccggtggga agcgcgtgcg caggatccag
     5461 tcgcggattt gcttgccggt cgagctcagt gccgcgacgt cctcgacgtc gagcgaggcg
     5521 agcgccttgg cgatgcgcgc gtcgagccgg tcctggcaga ggaactcacg gtaagcgtcg
     5581 gcggtcgtgg caaagccgca cggcacgctg acgccggctt gcgcgagcgc gccgatcatc
     5641 tcgccgagcg acgcgttctt tccgccgacg accgcgacat ccttgatccc gagccgcgag
     5701 aacggcacga cgtattcgcg ggcgacagag gtctcggcgg cgctcatgtg gcctctgggg
     5761 atcggttgcg cggcgcgcgg accagtggaa cactcggcga ggcgacgggc aacacccaag
     5821 ggttcgaaac gtgaggcgcc gcaccgttta tttcgtgtcg gaccaaaccg gcgtcacgtc
     5881 cgaaaccgtc gggcacagcg tgctcgcgca gttcgcgggg ctcgaggtcc gcgaggtgac
     5941 gctgccgttc atctcgaacg tcgaaaaggc gcaggaggcc gtgcgccgga tcaatcgcac
     6001 ggcggaggag gagggcgcgc ggccgatcgt gttcagcacg ctcgtcgagg acgagctgcg
     6061 cggcatcgtc atgaaatgca acgcgctgtt tctcgactgc ttcgcgggtc tcgtcatccc
     6121 cctcgagcgc gagctcgagc gccggtcttc gcaccgcacg ggccgctcgc atccggtcgc
     6181 ggatgctgcc tacacgcttc gcatggatgc gatcaactat gcgctcgcga acgacgacgg
     6241 agccggcggc gactacggcc gcgccgacat cgtcctcgtc ggcgtgtcgc gctccggcaa
     6301 gacgccgacg tgcctctaca tggcgcttca atacggagtg ttcgcggcga actacccgct
     6361 gaccgacgac gagctcgagg ccggcgtgtt gccgcggcgc ctcgaggcgc atcgcaagaa
     6421 gcttcacgcc ctcacaataa agccggagcg tctccggcag attcgcgagg agcggcggcc
     6481 gcagagccgc tacgcttcgc cgcaacaagt gcaatacgag attcgcgcgg ccgaggcgat
     6541 cttctcgcgc tacggtatcg cggtcctcga cacgaccgaa tcgtcgatcg aggagatcgc
     6601 gagcaggatc ttgagcgttg cgggtgtcga gcgccggttg cggccgtgag agcgcggagc
     6661 ggcggtgcgc tttcgcgttc agcactagta gatatcggca tcgacgaccc gcgccgggcc
     6721 gcgattgaga tgacggcata gcgcgtggta cgcggcggcg tttttcggct cgctgcacca
     6781 gcgctcgaag ttttcacgat cctggaacgt gcaatgcgga cttgtgagcc acgcatgcca
     6841 acgcgcggcc ctgcgcagag cttgtgcggt atgcctaccc gtcatggccc ccgattttgc
     6901 aagcaccatg ccagttgcag cgggttttcg cgctccgctt cgccggcggg gaggccagcc
     6961 tatgggcctt tctgccgatt ccatggagcg ccgaggcggt tctaaatcgc ggcatgacga
     7021 aaaacactcc cgcgaagcgg cgggcgct
//
LOCUS       DASSNN010000228         2940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1791895, whole genome shotgun sequence.
ACCESSION   DASSNN010000228 DASSNN010000000
VERSION     DASSNN010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791895"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1107
                     /locus_tag="VFL84_04155"
     CDS             55..1107
                     /locus_tag="VFL84_04155"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HET7607846.1"
                     /translation="MTNKNTLLTALASLATLGAAAGASGQALPNPYRLVDGWAKLPGG
                     RELGAVGDVDIDADGRHVWAILRCDATGDKFGYECLDSDLDVVVKFAPDGNAVAMFGG
                     GMFIWPHGLEIDPQGNVWVTDAATTERTSSPAARGRGHQVVKFSPSGEVLMRLGTPGV
                     PGNDNSHFNAPSDVVVAPNGDVFVADGHDAATNNRVVKFDKDGKFLKAWGRPGEAPGE
                     FHALHAIAMDARGRVFVGDRFNNRIQLFDQEGTHLASWTQFGRPSGIFFDHLGRIIVA
                     DSESDDIENPGWDMGIRIGDAATGAVHEFILYPWGDPRVRAGNGAEFAVVDRDGNIYG
                     GEPRPRRLQKYVRVRP"
     gene            1104..2285
                     /locus_tag="VFL84_04160"
     CDS             1104..2285
                     /locus_tag="VFL84_04160"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET7607847.1"
                     /translation="MTRRPALEPISRRAFVAGAAATIAAAPWIVRAQGAPTLRFRTIA
                     DPDGWAESLRLKGDWLIFEISDGVLSGFGEASHSNDDARCRELAKQLFERHYRGFEPS
                     LEALARKEREIAALAPDFVTATAFSGLNQAFYDLLAKREQVPVWRLFRDKTTFQGLPL
                     YATIQRALRTRSAEEYAAIAGEVRAQGYKTFKCAPFEAVNGPERAVEKAAAGLATLAR
                     LREHFPELTLRVDFHERFRPQDFYSIVPELERLKLDWLEEPFTVGPSYDELRQHTRLR
                     ISGGELFWGRGRFEEIAEHGWCDVIMPDVKHVGGFGPLLDVLKMDAAKAGNIEISPHN
                     PSGPISTAASLHAAAVHPERVRSLEYSFQRQYTRGSTGEVIENGTLLLNDEPGWGVAP
                     Q"
     gene            complement(2279..>2940)
                     /locus_tag="VFL84_04165"
     CDS             complement(2279..>2940)
                     /locus_tag="VFL84_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET7607848.1"
                     /translation="IALLLSLARALPVYAVEQNAGAFTRGFRETRGERPVEIEGKTLL
                     VVGLGGIGTEVAKRAHGLGMHVIATRNSRREGPNYVEYVGLADEYRTLAARADVVVNS
                     TPLTPETRGMFDARFFAAMKDGAFFINIGRGESVVTADLTAALQAGTIGGAGLDVTDP
                     EPLPPGHPLWSMSNVIITPHIATSSDYRSERTFTLVVENVRRYVRGDAVLAVVDLAAG
                     Y"
BASE COUNT          460 a         1022 c         1013 g          445 t
ORIGIN      
        1 agagcgaggc gcgtgccgcc ggctggcgtc cgccgcgctg acgaggggta tgccatgacg
       61 aacaagaaca cgctgctcac ggcgctggct tcactcgcaa cgctcggcgc ggcggcgggt
      121 gcttccggcc aagcgttgcc gaatccgtac cgcctcgtcg acggctgggc gaagttgccg
      181 ggcggccgcg agctcggcgc cgttggcgac gtcgacatcg acgccgacgg ccggcacgtc
      241 tgggcgatcc tgcgctgcga tgccaccggc gacaaattcg gctacgagtg cctcgactcc
      301 gatctcgacg tcgtcgtgaa attcgcgccg gacggcaacg ccgtcgcgat gttcggcggc
      361 ggcatgttca tctggccgca cggcctcgag atcgacccgc aaggcaacgt ctgggtcacc
      421 gacgccgcga cgaccgagcg cacgtcgagc cccgcggcgc gcggccgcgg ccaccaggtc
      481 gtcaagttca gcccgagcgg cgaagtgctg atgcggctcg gcacgccggg cgtgcccggc
      541 aacgacaact cgcacttcaa cgcgcctagc gacgtcgtcg tcgcgccgaa cggcgacgtc
      601 ttcgtcgcag atggccacga cgccgcgacg aacaaccgcg tcgtcaagtt cgacaaggac
      661 ggcaagttcc tcaaagcgtg ggggcgcccc ggcgaggcgc ccggcgagtt ccacgcgctg
      721 cacgcgatcg cgatggatgc gcgcggccgc gtgttcgtcg gcgaccgctt caacaaccgc
      781 attcagctgt tcgatcagga aggcacgcac ctcgcgagct ggacgcagtt cggccggccg
      841 agcggcatct tcttcgatca tttggggcgc atcatcgtcg ccgactccga gtccgacgac
      901 atcgagaatc ccggctggga catgggcatc cgcatcggcg acgccgcgac gggcgccgtg
      961 cacgagttca tcttgtaccc gtggggcgac ccgcgcgtgc gcgccggcaa cggcgccgag
     1021 ttcgccgtcg tcgatcgcga cggcaacatc tacggcggcg agccgcggcc gcgccggctg
     1081 cagaaatacg tgcgcgtccg gccgtgactc gccgccctgc gctcgagcct atatcgcgcc
     1141 gcgcgttcgt cgccggcgcg gccgcaacga tcgccgcggc tccgtggatc gtgcgcgcgc
     1201 agggcgcgcc gactttgcga ttccgcacca tcgcggatcc cgacggctgg gccgagagcc
     1261 tgcggctcaa aggcgactgg ctcatcttcg agatcagtga cggcgtcttg tccggcttcg
     1321 gcgaggcgtc gcacagcaac gacgacgcgc gctgccgcga gctcgcgaag caactcttcg
     1381 agcggcacta ccgcggcttc gagccgtcgc tcgaggctct cgcgcgcaag gagcgggaga
     1441 tcgccgcgct cgcgccggac ttcgtcaccg cgacggcgtt ctccgggttg aaccaagcgt
     1501 tctacgatct gctcgccaag cgcgagcaag tccccgtatg gcggctgttt cgcgacaaga
     1561 ccacgtttca gggcttgccg ctctacgcga cgatccagcg cgcgctcagg acacgcagcg
     1621 ccgaggagta cgccgcgatc gccggcgaag tgcgcgcgca gggatacaag acgttcaagt
     1681 gcgcgccgtt cgaggccgtc aacggccccg agcgcgccgt cgagaaagcc gcggcggggc
     1741 tcgcgacgct cgcgcgctta cgcgagcact ttcccgagct cacgctgcgc gtcgactttc
     1801 acgagcgatt tcggccgcag gacttctatt cgatcgtgcc cgagctcgag cgcttgaagc
     1861 tcgactggct cgaagagccg tttacggtcg ggccaagcta cgacgaattg cggcagcaca
     1921 ctcgcttgcg catttcgggc ggcgagctgt tttggggccg cgggcggttc gaggagatcg
     1981 ccgagcacgg ctggtgcgac gtcatcatgc ccgacgtcaa gcacgtcggc ggtttcggcc
     2041 cgttgctcga cgtgctgaag atggacgccg cgaaagccgg aaacatcgag atctcgcccc
     2101 acaacccgtc ggggcctatc tcgaccgcgg cgagcctgca cgcggcggcc gttcaccccg
     2161 agcgcgtgcg ctcgctcgag tactcgttcc agcgacagta cacgcgcggc agcaccggcg
     2221 aggtcatcga gaacggcact ctattgctga acgacgagcc cggctggggc gtcgcgcctc
     2281 agtagcccgc ggcgagatcg acgacggcca agaccgcgtc gccgcgcacg taacggcgca
     2341 cgttctcgac gacgagcgtg aaggtgcgct cactgcggta gtcggagctc gtcgcgatgt
     2401 gcggcgtgat gatgacgttg ctcatgctcc agagcggatg gcccggcggc agcggctcgg
     2461 ggtccgtgac gtcgaggccc gcgccgccga tcgtgccggc ctgcagcgcc gccgtgaggt
     2521 cggccgtgac gacgctctcg ccgcggccga tgttgatgaa gaacgcgccg tccttcatcg
     2581 ccgcgaagaa ccgcgcgtcg aacatgccgc gcgtttccgg cgtcagcggc gtcgagttga
     2641 cgacgacgtc ggcacgcgcg gcgagcgtcc ggtactcgtc ggcgagaccg acgtactcga
     2701 cgtagttggg gccttcgcgg cggctgttgc gcgtcgcgat cacgtgcatg cccagaccgt
     2761 gcgcgcgctt cgcgacctcg gtgccgattc cgccgaggcc gacgacgagc agcgtcttgc
     2821 cttcgatctc gacgggccgc tcgccgcgtg tctcgcgaaa gccgcgcgtg aacgcgccgg
     2881 cgttttgctc cacggcgtag accggcaacg cgcgcgccag tgagagcagc aacgcgatcg
//
LOCUS       DASSNN010000229         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1791980, whole genome shotgun sequence.
ACCESSION   DASSNN010000229 DASSNN010000000
VERSION     DASSNN010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791980"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFL84_04170"
     CDS             complement(<1..364)
                     /locus_tag="VFL84_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7607849.1"
                     /translation="MTPRLVTVAVLGAAGLGLAAMLSAQTGKRTLRDGVFSAAQAERG
                     ARVFASICTDCHELAEFTAAGAYLEEVEGESLWETFDFVSTKMPDDDPGSLRPDEYAA
                     VLAYIFKAYGLPAGAAELS"
     gene            complement(374..922)
                     /gene="def"
                     /locus_tag="VFL84_04175"
     CDS             complement(374..922)
                     /gene="def"
                     /locus_tag="VFL84_04175"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006910009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET7607850.1"
                     /translation="MAIRPILRIGHPVLRQVAAPVTELGTPALRELVHDMLETMRAND
                     GAGLAANQIGVPTRVVVFGLTANPRYPSAEPVPLTVLVNPEIEVLDEERESGWEGCLS
                     VPGMRGLVPRYKRLRYRGFDELGGAIDRTVEGFHARVVQHECDHLDGILYPQRIEDMT
                     AFGFQDELVASGVLPLAEAAAD"
     gene            1078..>2184
                     /locus_tag="VFL84_04180"
     CDS             1078..>2184
                     /locus_tag="VFL84_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7607851.1"
                     /translation="MYHAHFGLEHGLCGDAIAADGDVFRTAKHDRLIAHFKLALASPS
                     SCIVLHGPAGVGKTTLTAAALRATSTRLAIAWLGGMATTASELLELLLVELGVSTVRT
                     TRMERVQLWRQFQGEMRATDSRLFIVVERTEDLSPEVLHALDQLTAPDAAGNPGANVV
                     FLGHATIFEHLASPLLDSLRQRIRLSTALEPFTEADLQDYLRHQVAAAGGNFERIFAT
                     GAVAALHRYSKGVVRLANTLCESALDIAAVQQQKQLTGELVAKTAVTLLGLAEPAAAV
                     QSAPGARSPAAHAPAPAAAPAAPRPAAHAAPIFERRSEPRLPTSVSPVPPEVRAAPAP
                     AAAPASAAIAPTPSKTDEIDFDGSITDVADVALAD"
BASE COUNT          347 a          798 c          718 g          321 t
ORIGIN      
        1 cgctgagctc ggcggcgccg gccggcaggc cgtaagcttt gaagatgtaa gcgagaacgg
       61 cggcgtactc gtccggccgc agcgaccccg gatcgtcgtc gggcatcttc gtcgagacga
      121 aatcgaacgt ctcccagagc gactcgcctt cgacttcctc gagataggcg cccgccgccg
      181 tgaactcggc gagctcgtga cagtccgtgc aaatcgacgc gaacactcga gcgccgcgct
      241 cggcctgcgc cgccgagaac acgccgtcgc gcagcgtgcg cttgcccgtc tgcgcgctca
      301 gcatcgccgc caggccgagg ccggcggcgc cgagaacggc gactgtgacg agcctcggtg
      361 tcatgtcagc gagtcaatcc gccgcggctt cggcgagcgg caacacgccg ctcgcgacga
      421 gctcgtcctg aaacccgaac gcggtcatgt cctcgattcg ctgcggatag agaatgccgt
      481 ccaagtgatc gcactcgtgc tggaccacgc gcgcgtgaaa gccttcgacg gtacggtcga
      541 tcgcgccgcc gagctcgtcg aaaccgcgat agcgcagccg cttgtagcgg ggcaccagac
      601 cgcgcatacc gggcacgctc aagcagcctt cccatcccga ctcgcgctcc tcgtcgagga
      661 cctcgatctc ggggttcacg agcacggtca gcggcaccgg ctcggccgac gggtagcgag
      721 gattcgccgt caagccgaag acgacgactc gcgtcggcac gccgatctgg ttcgccgcga
      781 ggccggcgcc gtcgttggcc cgcatcgtct cgagcatatc gtgaaccagc tcacgcaacg
      841 ccggcgtgcc gagctccgtg acgggagccg cgacctgccg cagcacgggg tgacctatac
      901 gcaaaatggg ccgaatcgcc atcgtccgaa acgttcctag cgccgtttgg cgcaattata
      961 gagagccgcg gcgccggctg tcgttttgca taagttgtga cgccgtttca cgaaccgggc
     1021 ggcccggcac ggcacacttt agccctgcca aaccgtcggc gaagggcacg ggcggctatg
     1081 taccacgcgc atttcggtct cgagcacggg ctctgcggtg acgcgatcgc ggccgacggc
     1141 gacgtctttc gcacggcgaa gcacgatcgg ctcatcgcgc acttcaagct cgcgctcgcg
     1201 agcccgagct cgtgcatcgt tctgcacgga cccgccggcg tcggcaagac gacattgacg
     1261 gcggccgcgc tgcgcgcgac cagcacgcgg ctcgcgatcg cgtggctggg cggcatggcg
     1321 acgaccgcgt cggagctgct cgagctgctg ctcgtcgagc tcggcgtcag cacggtgcgc
     1381 acgacgcgca tggagcgcgt gcagctctgg cggcaatttc aaggcgagat gcgcgcgacg
     1441 gactcgcggc tattcatcgt cgtcgagcgc acggaggacc tctcacccga ggtgctgcat
     1501 gcgctcgacc agctcaccgc gccggacgcg gcgggcaatc ccggcgcgaa cgtcgtgttc
     1561 ctcggccacg cgacgatctt cgagcacctc gcctccccgc tgctcgactc tctgcgacaa
     1621 cgcatccggt tgagcaccgc gctcgagccg ttcaccgaag ccgatctcca ggactacttg
     1681 cgtcatcagg ttgcggcggc gggcggcaac ttcgagcgga tcttcgctac cggcgccgtc
     1741 gcggcgctgc accgctattc gaagggcgtc gtgcggctcg cgaacacgct ctgcgagagc
     1801 gcgctcgata tcgcggccgt ccagcagcaa aagcagctga ccggagagct cgtcgcgaag
     1861 acggccgtga cgttgctcgg tctcgccgag cccgccgcgg cggttcaatc cgcgccgggc
     1921 gcacgatccc ccgccgcgca cgcaccggcg cccgcagccg cgcctgccgc gccgcgcccc
     1981 gcggcacatg cggcgccgat cttcgagcgc aggagcgagc cgcgcttacc gacgtccgtt
     2041 tcgcccgttc cgcccgaggt ccgcgccgcg ccggcgccgg ccgctgcgcc cgcctcggcc
     2101 gcaatcgcgc ccacgccgtc caagaccgac gagatcgact tcgacggcag catcacggac
     2161 gtcgccgacg tcgcgctcgc cgac
//
LOCUS       DASSNN010000230         3263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1430021, whole genome shotgun sequence.
ACCESSION   DASSNN010000230 DASSNN010000000
VERSION     DASSNN010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430021"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFL84_04185"
     CDS             <1..531
                     /locus_tag="VFL84_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET7607852.1"
                     /translation="DHELNVSTFTARCVASAGASPYNVTIAGLAALEGTKHGGATARV
                     DASWDALRQTSDLRAALAERLRRGEAIEGFGHPLYPGGDPRAALLLSMLPKGKTASFA
                     RELAAAAKAVLGEAPTIDFALVALARALGLPSGSALTLFAIGRTIGWIGHAIEQYEAN
                     AIIRPRAKYVGATPVA"
     gene            568..1047
                     /locus_tag="VFL84_04190"
     CDS             568..1047
                     /locus_tag="VFL84_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7607853.1"
                     /translation="MPTAEPPRPPFPPFTPETAAQKVRMAEDAWNTRDPQRVALAYTP
                     DSVWRNRSEFLAGRDAIREFLARKWAKELDYRLVKELWACHGNRIAVRFAYEWRDDGG
                     KWYRSYGNENWEFDERGFMQRRIASINDLPIAAGERKLLWPLGRRPDDHPGLTALGL"
     gene            1126..1566
                     /locus_tag="VFL84_04195"
     CDS             1126..1566
                     /locus_tag="VFL84_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607854.1"
                     /translation="MNIDRQAIIDAEHLRLLSIFHFVAAGLAMIGGGFASLYFVMFQL
                     IFANPEIWAQSEQGAPPEQMMTFFRSFLLGFMLWFLIAAVGNLLSGLFMRGRRHRVFS
                     MVVAGINCLHIPIGTLLGVFTFIVLSRDSVRKMYEEVASHAKGV"
     gene            1550..1861
                     /locus_tag="VFL84_04200"
     CDS             1550..1861
                     /locus_tag="VFL84_04200"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HET7607855.1"
                     /translation="MRKVFSSVEPSETVLIRDALLHRGVAATIQNEFSGRTAAPGFRP
                     PAEVWVTNDHDYDEARRVVVEALAKIDSKLDAAPWVCPNCREENPQSFDICWNCARER
                     P"
     gene            1868..>3263
                     /locus_tag="VFL84_04205"
     CDS             1868..>3263
                     /locus_tag="VFL84_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7607856.1"
                     /translation="MATPPGVSERDFARALTELRNAVGADWVFTSDEDLNLYRDAYSP
                     FKGEARDPVPSAAVAPTSVEQVQAIVRIANTHKIPLWTISTGRNLGYGGSAPAYSGSV
                     IVDLKRMNRIIEVNDKAHYAVVEPGVSYFDLYRFIEERGLKVWIDPADPGWGSPLGNA
                     LERGGGRTPMRDHWNAVCGLEVVLANGEILRTGMGAMENSEVWHQYKYGFGPLVDGLF
                     SQSNFGIVTRMGLWLMPSPEGYRDGRVNVAQHDDLVPFMETYAYLMNSDILRGTMLLE
                     SPLLNYHVTQGGSFFEMPNGPSTPELDRLARERNLPYWSAELRFWGPPRVIDAQWEYV
                     KEKFGRIPGATFAEGHRYAFPAAVDQNDPTLTVALGIPSLGVFGLLRGGGHLGFSPII
                     PMTGDAVFRAQKAFGRLYAELALPAANQFAALPWSYNPRALVLLFGLPMSQDKEQNRQ
                     SREIFERLVEVSAEN"
BASE COUNT          548 a         1097 c         1051 g          567 t
ORIGIN      
        1 gaccacgagc tcaacgtctc gacgttcacg gcacgctgcg tcgcgtccgc gggagcgtcg
       61 ccgtacaacg tcacgatcgc cggtctcgcc gcgctcgagg gaacgaagca tggcggcgcg
      121 acggcgcgcg tcgacgcgtc gtgggacgcg ctgcgccaga cgagcgactt gcgcgccgct
      181 cttgcggagc gcctacgccg cggcgaggcc atcgaaggct tcggccaccc gctctatccc
      241 ggcggcgatc cgcgcgccgc gctgctgctt tcgatgttgc cgaagggcaa gacggcgtcg
      301 ttcgcgcgcg agctcgccgc ggccgccaaa gccgtcctcg gcgaagcgcc gaccatcgat
      361 ttcgcgctcg tcgcgctcgc gcgcgcgctc ggcttgccga gcggctccgc gctgacgctg
      421 ttcgcgatcg gccgcacgat cggctggatc ggccacgcga tcgagcagta cgaagcgaac
      481 gcgatcatcc ggccgcgcgc gaagtacgtc ggcgcgacgc ccgtcgcata acatctagtc
      541 gttgaaacga ttcgtaaacc gccgcacatg ccgaccgccg agccgccccg ccccccgttc
      601 ccgccgttca cgcccgagac cgcggcacag aaggtgcgca tggccgagga cgcctggaac
      661 acgcgcgatc cgcaacgcgt cgcgctcgcc tatacgcccg acagcgtgtg gcgcaaccgc
      721 tccgaattct tggcgggccg tgacgcgata cgggaattcc tcgctcgcaa atgggccaag
      781 gagctcgact atcgcctggt caaggagctc tgggcctgcc acggcaaccg catcgccgtg
      841 cgctttgcgt acgaatggcg tgacgatggc ggcaagtggt atcgctcgta cggcaatgag
      901 aattgggagt tcgacgagcg cggcttcatg caacggcgca tcgcgagcat caacgatctg
      961 ccgatcgccg ccggcgagcg caagctcctc tggccgctcg gccggcgccc cgacgaccat
     1021 cccgggctga ctgcgctagg cctgtaacga tcgcctcgat ctgatacttt ggacgcctgg
     1081 cgctgggccc attttcaatg ggccctactc ggggggagcg aagccgtgaa catcgaccgt
     1141 caggccatca tcgacgcaga gcacctgcgc ctgctttcca ttttccactt cgttgcagct
     1201 ggcctcgcca tgataggcgg cggcttcgcc tcgctgtact tcgtcatgtt ccagctgata
     1261 ttcgcgaatc ctgaaatttg ggcgcagtcg gaacagggcg cgccgccgga acagatgatg
     1321 acgttctttc ggtcgttcct gttgggcttc atgctctggt tcctgattgc cgccgtgggc
     1381 aacctgctct cggggttatt catgcgcggc aggcgacacc gagtattctc gatggtggtc
     1441 gcaggcatca attgtctgca catcccgatc ggcacgctgc tcggagtatt cacgttcatc
     1501 gttctcagtc gtgattccgt gcggaagatg tacgaagagg tggcgagcca tgcgaaaggt
     1561 gtttagctca gttgagccca gcgagaccgt gctgattcgc gacgcgctgc tccaccgcgg
     1621 cgtcgctgcc acgatccaga acgaattctc gggccgaacg gcggcaccgg gatttcgccc
     1681 gcccgccgag gtgtgggtca cgaacgatca cgactacgac gaagcgaggc gcgtcgtcgt
     1741 cgaggcgctc gcgaagatcg acagcaaatt ggacgcggcc ccttgggttt gcccgaattg
     1801 ccgcgaggaa aatccgcagt cgttcgacat ctgctggaac tgcgcgagag aacgacccta
     1861 gaagacgatg gcaacgccac ccggggtttc cgaacgcgat ttcgcgcgcg cgctcaccga
     1921 gctgcgcaat gccgtcggcg ccgactgggt gttcacgtcg gacgaggacc tgaacctcta
     1981 ccgcgacgcg tactcgccgt tcaaaggcga agcccgcgac cccgtgccgt ccgcggccgt
     2041 cgcgccgacc agcgtcgagc aggtgcaagc gatcgtgcgc atcgcgaaca cgcacaagat
     2101 tccgctctgg acgatctcga cgggccggaa cttgggctac ggcggctctg cgcccgcgta
     2161 ttccggcagc gtcatcgtcg acttgaagcg catgaaccgc atcatcgagg tcaacgacaa
     2221 ggcgcactac gctgtcgtcg agcccggcgt cagctacttc gacctctatc gtttcatcga
     2281 ggagcgcggc ctcaaagtct ggatagatcc tgccgatccg ggctggggct cgccgctagg
     2341 caacgcgctc gagcgcggcg gcggccgcac gccgatgcgc gatcactgga acgccgtctg
     2401 cggcctcgag gtcgtgctcg cgaacggcga aatcttacgc accggcatgg gcgcgatgga
     2461 gaactccgag gtctggcatc aatacaagta cggcttcggg ccgctcgtcg acggcctgtt
     2521 ctcgcagtcg aacttcggta tcgtcacgcg catgggcctc tggctcatgc cctcgcccga
     2581 gggctaccgc gacggccgcg tcaacgtcgc gcagcacgac gacctcgtgc cgttcatgga
     2641 gacgtacgcg tacttgatga actcggacat cttgcgcggc acgatgctgc tcgagagccc
     2701 gttgctgaac tatcacgtca cgcaaggcgg ctcgttcttc gagatgccga acggcccgtc
     2761 tacgcctgag ctcgaccgcc tggcgcgcga gcggaatctg ccgtactgga gcgcggagct
     2821 ccgcttctgg gggccgccgc gcgtcatcga cgcgcagtgg gaatacgtca aggagaagtt
     2881 cggccgcatt cccggcgcga cgttcgcgga gggccaccgc tacgcgttcc cggccgccgt
     2941 ggatcagaac gacccgacgt tgaccgtcgc gctcggcatc ccgtcgctcg gcgtgttcgg
     3001 cttgctgcga ggcggcggcc atctcggctt ctcgccgatc attccgatga cgggcgacgc
     3061 ggtgttcaga gcgcagaaag cgttcggccg cctctatgcc gagctcgcgc tgccggcggc
     3121 gaatcagttc gcggccctgc cgtggtcgta caacccgcgc gcgctcgtgc tgctgttcgg
     3181 cttaccgatg tcgcaggaca aggagcagaa ccgccagagc cgcgagatct tcgagcgcct
     3241 cgtcgaagtc tcggccgaga acg
//
LOCUS       DASSNN010000231         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1792317, whole genome shotgun sequence.
ACCESSION   DASSNN010000231 DASSNN010000000
VERSION     DASSNN010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792317"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFL84_04210"
     CDS             complement(<1..348)
                     /locus_tag="VFL84_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7607857.1"
                     /translation="MEYRNLGRTGVKVSQLCLGTMMFGRSANEQDSIAIVHAALERGV
                     NFVDTADAYSAGASEQFVGKALSNGRRAPVVLATKAFFPQDQKDPNARGLSRRHLIEA
                     CEASLKRLQTDWID"
     gene            complement(393..899)
                     /locus_tag="VFL84_04215"
     CDS             complement(393..899)
                     /locus_tag="VFL84_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HET7607858.1"
                     /translation="MSISIYDQTIPVFTQMLSALDKVLSKAEADAAARKIDLDVFVNA
                     RLAPDMLPLTRQIQIMTDQAKGCASRLAGQDPPKWPDDEKTFADLHARVAKTIVHLKS
                     FKPEQFEGAEKRAIEIKFPNATFNFTGKDYLLMFVTPNFYFHYTTAYAILRHNGVPIG
                     KGDFLGSR"
     gene            complement(1013..1813)
                     /locus_tag="VFL84_04220"
     CDS             complement(1013..1813)
                     /locus_tag="VFL84_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607859.1"
                     /translation="MQASAFGCRVLLAVSWLVLVAGCDSGSSSPVPQPVAAPPPPQPP
                     APPADPPPPPPQQPPNSPQDPPPPPADPPPPPAPPPHTPPPPPDDALPPLASVVVNVA
                     DNHQVGSPYWSDGNTSTGGAGPPVQGLRCGDMVETYHVHTHVSFFLNGDQLAMPSRIG
                     NVSPRPGSSCYYPLHTHDRTGRVHVEAEAPGTFTLGQFFAIWGQPLEPMNVAGHPGVP
                     VVIYIVDGNSALRYEGDFAAIELTSRRGIVIQIGTKIAEIPRYTWFGP"
     gene            complement(1919..>2968)
                     /locus_tag="VFL84_04225"
     CDS             complement(1919..>2968)
                     /locus_tag="VFL84_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter family protein"
                     /protein_id="HET7607860.1"
                     /translation="RCGHGELLVLFGAALAIGGGEAFASLGLSSELGALLLGTMLADH
                     RRSQELTNVLWSLKELFLVGFFLNIGFTSDLPTLGSLTSALLLVLLLPIKGAIFFLLL
                     IGLGLRARTSFLTAVSLMTYSEFALIVVTLGADNGVLGREWVVVTALAVALSFIAAAP
                     FNAFAHDLFRTASPWLERLEREKRHPDDEPISLGRAEILIVGMGRVGSGAYDYLRLQN
                     ENIVGIDSDPAKIESNVREGRRVAYADAEDPSFWQRLNIDRLRAIMLAVPDLQAKVTA
                     AHALRRRGFRGLVSATHMYPEEREPILDAGCDVTYNYYTEAGVGFARHTYETLRPTST
                     AASTAAKPEKARQFQ"
BASE COUNT          496 a          950 c         1014 g          508 t
ORIGIN      
        1 atcgatccaa tcggtctgca agcgcttcaa cgacgcttcg cacgcctcga tcaaatgccg
       61 gcggctcaag ccgcgcgcat tcggatcctt ctggtcctgc gggaagaacg ctttcgtcgc
      121 gagcacgacc ggcgcgcgcc ggccgttcga gagcgctttg cccacgaact gctcgctcgc
      181 tccggcggaa tacgcgtccg cggtgtcgac gaagttcacg ccgcgctcga gcgcggcgtg
      241 aacgatcgcg atcgagtcct gctcgttcgc gctgcgcccg aacatcatcg tgccgaggca
      301 cagctggctg accttgacgc cggtgcggcc gagattgcgg tattccatgc gctttgctcc
      361 gtcgaccgtc ccggcgctag gccatcgttc ggctagcggc tgccgaggaa gtcgcccttt
      421 ccaattggca cgccgttgtg gcgcaggatc gcgtacgcgg tcgtgtagtg gaagtagaag
      481 ttcggggtca cgaacatcag caggtagtcc ttgcccgtga agttgaaggt cgcgttcggg
      541 aacttgatct cgatcgcgcg cttctcggcg ccttcgaact gctccggctt gaaggacttc
      601 aagtgcacga tcgtcttcgc gacgcgcgcg tgcaagtcgg cgaacgtctt ctcgtcgtcg
      661 ggccacttcg gcggatcctg gcccgcgagc cggctcgcgc agcctttcgc ctggtcggtc
      721 atgatctgga tctgccgcgt cagcggcagc atgtcgggcg cgagacgagc gttgacgaac
      781 acgtcgagat cgatcttgcg cgcggcggcg tcggcctcgg ccttgctgag cactttgtcg
      841 agcgccgaca gcatttgcgt gaatacggga atggtctggt cgtagatgct gatggacacg
      901 gtgaatcctc ttcgaagagc tgattggtct aaagcgtgcg gcaatccgcg cggcgcgcgg
      961 gatagccggg cgacgcccgg ctcggcacct tatatatgtg tccatggcga gctcagggcc
     1021 cgaaccaggt gtagcgcggg atctcggcga tcttggtgcc gatctggatg acgattccac
     1081 gccgggacgt aagctcgatc gcagcgaagt cgccttcgta gcgcaacgcg ctgttcccgt
     1141 cgacgatgta aatgacgacg ggaacccccg ggtgacccgc gacgttcatc ggctcgagcg
     1201 gctggcccca aatggcgaag aactgcccga gcgtgaatgt gcccggcgcc tccgcttcga
     1261 cgtggaccct tccggtgcgg tcgtgcgtat gcagcgggta atagcaactg ctgcccggcc
     1321 tcggcgacac gtttccaata cggctcggca tcgcgagctg atcgccgttc aagaagaacg
     1381 agacgtgcgt gtgaacgtgg tacgtctcga ccatgtcgcc gcagcgcagg ccctgaaccg
     1441 gaggaccggc gcctccggtc gacgtattcc cgtcgctcca atacgggctg ccgacctggt
     1501 ggttgtcggc gacgttgacg acgacgctcg caagcggcgg gagcgcgtcg tccggcggcg
     1561 gaggtggcgt gtgcggaggc ggtgccggtg gcggcggcgg atcggccggc ggcggtggcg
     1621 gatcttgcgg tgaatttggc ggctgctgcg gaggaggagg cggcggatcg gccggcggcg
     1681 ccggcggctg cggtggcggc ggtgccgcga cgggctgcgg caccgggctc gacgagccac
     1741 tgtcgcaacc tgcgacgaga acgagccaac taaccgcgag cagcacccga cagccgaaag
     1801 ccgacgcttg catcgctccc tctccgactg ggccggagca ctaggtgcaa gagttgggcc
     1861 agtcgcagcg tcgcgtcggc atcacgcacc cccgcgtgag cgcacaacga ttttcacgtt
     1921 attggaactg gcgcgctttt tctggcttcg ccgcggtgct cgcggcggtc gacgtcgggc
     1981 gcagcgtctc gtatgtgtgg cgcgcgaagc cgacaccggc ttccgtgtag tagttgtaag
     2041 tcacgtcgca gcccgcatcg agaatcggct cgcgctcctc ggggtacatg tgcgtcgcgc
     2101 tcaccaggcc tctgaagcca cggcgccgca acgcgtgcgc cgccgtgact ttcgcctgca
     2161 gatcgggcac tgcgagcatg atcgcgcgca ggcgatcgat gttcagccgt tgccaaaagc
     2221 tcgggtcctc ggcgtcggca tacgcgacgc ggcggccctc gcgaacgttc gactcgatct
     2281 tcgccgggtc gctgtcgatg ccgacgatgt tctcgttctg caagcgcagg tagtcgtacg
     2341 cgccggagcc gacacgcccc atgccgacga tgaggatctc ggcgcgaccg agcgagatcg
     2401 gctcgtcgtc gggatggcgc ttctcgcgct cgaggcgctc gagccacggg ctcgccgttc
     2461 ggaacaagtc gtgcgcgaac gcgttgaacg gcgcggccgc gatgaacgac agcgcgacgg
     2521 cgagcgccgt cacgacgacc cactcgcggc cgagcacgcc gttgtcggcg ccgagagtga
     2581 cgacgatcag cgcgaactcg ctgtatgtca tgaggctcac ggcggtcaga aagctcgtgc
     2641 gcgcccgcaa accgaggccg atcagcagca ggaagaagat cgcgcctttg atcggcagga
     2701 gcaaaacgag cagcaatgcg ctcgtcaacg accccaacgt cggaaggtcg ctcgtgaaac
     2761 cgatgttcag gaagaagccg acgagaaaca gctccttcag gctccagagc acgttcgtga
     2821 gctcctgcga gcgccgatga tcggcgagca tcgtgccgag cagcagcgct ccgagctcgg
     2881 agctcaggcc caacgacgcg aacgcttcgc cgccgccgat cgcgagcgcg gcgccgaaca
     2941 gcacgagcag ctcgccgtgg ccgcagcg
//
LOCUS       DASSNN010000232         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1054416, whole genome shotgun sequence.
ACCESSION   DASSNN010000232 DASSNN010000000
VERSION     DASSNN010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054416"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            625..2379
                     /locus_tag="VFL84_04230"
     CDS             625..2379
                     /locus_tag="VFL84_04230"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET7607861.1"
                     /translation="MRSEMLTMELDAESPRLSVTGSGRVALTPEMDAELTLNFSDTSL
                     DPYLRFFEPRLSPFTTAVAGGTIHATGELSDIDHLIVDATVNTLDLKLFDYSLHNAGP
                     LKLALDRHRVEVQQFKLEGDGTSLGLDGEISLHDNRIALTASGDANLSILQGFFRELR
                     SSGNASLKASIEGPLDAPVFSGAADIADGRIRYTALPHSLQNINGHLSFDAQGIRVDT
                     ATAELGGGRVQFGGRMGIEGFTLGNFDLTATGERMNLRYPEGFRSVVDASLALRGPIA
                     SPVLSGTVTVRNGVYESKFDPTSNILSLASGSSSLPRAVNTEPTIPLRYDIQVNAPVG
                     SLTIKNALAQITASANLTLSGTYDKPQLFGRADIERGEVFFEGNRFVVTRGTVDFYNP
                     TAIEPFFDIEAETRVHVPSADETYRITLAISGTVGGHLNFSANSDPPLTPVDILSVLF
                     GQSTDVSNPELRALNQRDVTQSEEQLLRAGLLQVLAGPLAAPVTRAVEQTFGINTVQL
                     APSLGTQNDPLTPTARLIIGKRISDRAYLTFSRALGTTQREQIIVLEYDQSDRLGWIL
                     TQTGDRTFSIDFRVRRSF"
     gene            2384..>2970
                     /locus_tag="VFL84_04235"
     CDS             2384..>2970
                     /locus_tag="VFL84_04235"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HET7607862.1"
                     /translation="MVPLLVLLLLQAGTTGAPQAAPNPQQPAAAAYVGERVASVSLLI
                     EGRPTTEPALSDVIATPVGAPLSLTDVRETITHLYSLGRFDDVQVDAEHTPDGGVALR
                     YLLTPIHAVSDVEFKGNLGVSESALRRRMTDRFGPTPRIGLAADVSSTLEQYLHEQGY
                     LAASVRVAPPILMHNPDRTTLVFTVSAGPRARVTK"
BASE COUNT          512 a         1041 c          935 g          482 t
ORIGIN      
        1 cagaacagct acgtgttcat ctcgaggagc cgcattcgag agggagactc cgagatcaat
       61 gccgacggca agttctcgct cggctacccg cggaaggatc atggcgagga gatcaacgcg
      121 acggtgcgtg tggtcaaacg gccgctcgcc gaccttcggc acgccttcga gctcgacgac
      181 tacccggtgg acggcgccgc ttcgggcgag tatcggctga ccggcgctta cacgacgccc
      241 caggggtatg ggcggctgca gatcgatcgg ggcactgcgt atggcgagac gttcgacacc
      301 gcgacggcgg atctgcgttt cgaaagcacg ggtacgcccg gcgtgcggct ggacaacatc
      361 gcaatcgcga aaagcacagg caggatgacg ggcgcggcct tcgtcggatg ggacggcacc
      421 tacagcttcg acgcgaacgc gctgaagatt cccgtcgaat cgctcgtgac gctctcgttt
      481 ccgcgggcgc cgctgtccgg catcctgcag ttcacggcaa cggggaccgg cacgttcgac
      541 gcgccccgct acgacgtcag gctgcaggtt gccgatctct tcgccggcga cgaaggcatc
      601 ggccaggtcc gcggccgcct ctcgatgcgc agcgagatgc tgacgatgga actggacgcg
      661 gaatctccgc gtctatcggt caccggctcg ggccgcgtcg ccctcacgcc cgaaatggac
      721 gccgagctga cgctcaactt ctcggacacg tctcttgatc cttaccttcg gttcttcgag
      781 ccgcgcctgt cgccgttcac gaccgccgtc gcgggaggaa cgattcacgc caccggcgag
      841 ctgtccgaca tcgatcacct gatcgtggac gcgacggtga acacgctcga tctcaagctg
      901 ttcgactact cgctgcacaa cgccggcccg ttgaagctcg cgctcgaccg gcaccgcgtc
      961 gaagtgcagc agttcaagct cgaaggcgac ggcacgtcgc tcggcctcga cggagaaatc
     1021 agccttcacg acaaccgcat cgcgctgacc gcgtcgggcg acgcgaacct cagcatcctg
     1081 cagggctttt tccgcgagct ccgcagctcc ggcaacgctt cgctcaaggc ctcgatcgaa
     1141 ggaccgctcg acgcgcccgt cttctcgggt gcagccgaca ttgccgacgg acgcatccgg
     1201 tacacggcgc tcccgcacag ccttcagaac atcaacggcc acctttcgtt cgacgcgcaa
     1261 ggcatccgcg tcgatacagc caccgccgaa ctcggcggag gacgggtgca gttcggcggc
     1321 cgaatgggca tcgaggggtt cacgctcggc aatttcgatc tcaccgcgac gggcgaacgg
     1381 atgaacctgc ggtatccgga aggattccgg tccgtggtgg acgcgtcgct ggcgcttcgc
     1441 ggcccgatcg cgtctcccgt cctcagcggc accgtcaccg tgcgcaacgg cgtgtacgag
     1501 agcaagttcg atccgacgtc gaacatcctc agcctggcga gcggttcctc gtcgctgccc
     1561 cgtgccgtca acaccgagcc cacgattccg ctgcggtacg acatccaggt caacgcaccg
     1621 gtgggaagcc tcacgatcaa gaacgcgctg gcgcagatca cggcaagcgc gaacctgacg
     1681 ctgagcggca cctatgacaa gccgcagctg ttcggccgcg ccgatatcga acgcggcgaa
     1741 gtgttcttcg agggcaaccg cttcgtcgtc acgcgcggca cggtggattt ctacaacccc
     1801 accgccatcg agccgttctt cgacatcgaa gcggagaccc gcgtccacgt gccgagcgcg
     1861 gacgagacgt atcgcatcac gctcgcgatc agcgggacgg tgggcggcca cctgaacttc
     1921 tcggccaact ccgatccgcc gctgacgccg gtggacatcc tgtcggtgct gttcggacag
     1981 tcaaccgacg tctcgaaccc cgagctgcgc gcgctcaatc aacgcgacgt gacgcagtct
     2041 gaagagcagt tgctgcgcgc gggcctgctg caggtgctgg ccgggccgct cgccgcgccg
     2101 gtcacccgcg ccgtcgaaca gacgttcggc atcaacaccg tccagctcgc gccgagcctc
     2161 ggcacgcaga acgacccgtt gacgccgacg gcgcgcctca tcatcggcaa gcgcatatcc
     2221 gatcgcgcgt acctgacgtt ctcccgcgcg ctcggcacca cgcagcgcga gcagatcatc
     2281 gtcctcgaat acgatcagag cgaccgcttg ggctggattc tcacgcagac gggcgatcgc
     2341 acgttctcga tcgacttccg ggtgcgccgc agcttctgac gtgatggttc cgcttctcgt
     2401 tcttcttctg cttcaggccg ggacgaccgg cgcaccgcag gccgcgccca acccacagca
     2461 gccggcagcc gccgcgtacg tcggtgagcg cgtcgcgtcg gtctcgctgc tgatcgaggg
     2521 tcggccgacg accgagcccg cgctgtccga cgtcattgcc acgccggtcg gcgccccgct
     2581 gtcgctgacg gacgtgcgcg aaacgatcac gcacctctac agcctcggcc ggttcgacga
     2641 cgtgcaggtc gatgcggagc acacgccgga tggcggcgtg gcactgcgct acctgctcac
     2701 gcctatccac gccgtttcgg acgtcgagtt caaggggaat cttggcgtct ccgagtcggc
     2761 gctccggcgg cggatgacgg atcggttcgg cccgacgccg cgcatcggcc tggcggcgga
     2821 cgtgtcctcc acgctcgagc aatacctgca cgagcaggga tatctcgcgg caagtgtgcg
     2881 cgtggccccg cccatcctca tgcacaaccc cgaccgcacg acgctcgtct tcaccgtcag
     2941 cgcgggtccg cgggcgcgcg tgacgaagat
//
LOCUS       DASSNN010000233         2810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_908936, whole genome shotgun sequence.
ACCESSION   DASSNN010000233 DASSNN010000000
VERSION     DASSNN010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908936"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1336
                     /locus_tag="VFL84_04240"
     CDS             263..1336
                     /locus_tag="VFL84_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7607863.1"
                     /translation="MTGLLPAALAAQPRTTLDIYVIDVEGGNATLFVAPYGESLLIDT
                     GNGGQAAARDAGRIMAAVQDAALTRIDHLITTHYHGDHFGAMSELAGRIPIGEFIDHG
                     ANVQPNPATDTFLNDVYPALYAKSKHTVVKPGDRVAMRGVDVRIVASAGEAIRDAVPG
                     AGQPNPRCASHQPKDQDPSENAQSVGSHFTFGRFRALHLGDLTWNKEFELACPRNLLG
                     TVDLFVVTHHGQPASNAPVLVHALAPRVAIMNNGTRKGGQPDTMKVLFSSPGLEDLWQ
                     LHFSLLSGQEDTVPGAFIANVADEEPASRPTEPAAAPAPGTNAAPPPAHNGPAFWIKV
                     AAHDDGSFTVTNPRNGFSKHYAR"
     gene            complement(1404..1955)
                     /locus_tag="VFL84_04245"
     CDS             complement(1404..1955)
                     /locus_tag="VFL84_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7607864.1"
                     /translation="MKTFARPFSGALLGALGAACLALTIGSGARAEEVTAETLVDRLQ
                     IQDLITRYYNNFGRENGENFADFYAEDAELILGERHFKGRNGILEAYGRAPGQAPRPA
                     QPPRFSFIVAIDNPLIVVHGGSNTATAQLVFTEYVIEKQGEPVKVITQGKEYSTFVKV
                     AGHWRYKTRQIKAGTELPEGWKE"
     gene            2052..>2810
                     /locus_tag="VFL84_04250"
     CDS             2052..>2810
                     /locus_tag="VFL84_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607865.1"
                     /translation="MAPRLVGSLGTLATLVGATLLCSQPSAGQEQSAAPPLVVTAFGD
                     KPAPPYTVPRTSWGDPDLQGAWSSDDTSGVPMSRREEHGDRLYLTDEELAERTQQVER
                     GVQRAENEATSSFRGDFARRALPQTSLIVDPPNGRVPAYTAEGMKRPMPRGTYGNGPL
                     DSYTDFSGYERCITRGIVGSTLRVVYGNGQRIVQAPGVVALSYEMVHDTRLIYTDGRP
                     HIGQAIRQYLGDSRAHWEGDELVVETTNLTDRTAI"
BASE COUNT          463 a          965 c          947 g          435 t
ORIGIN      
        1 cggttgtaga gcggccagtt cgcggccgcg acgctctccg ccgctgcggc cggggacgcg
       61 gcgctggccg cgggtagttc cggcgcggaa tccgtgccgc cgcagcccgc caagagctga
      121 gcgcaacacg ctgcggcgat gcagaatcgg aagccctggt tcgttttcat tggatgagct
      181 ccgcggctgg caaggcgtac gataccgggc gccgcttctg cggaggagac acccgatgag
      241 ccgccgaacg agtctagcac tcatgaccgg gctgctgccc gccgcgctgg cggcacagcc
      301 gagaacgacg ctcgacatct acgtgatcga cgtcgagggc ggcaacgcga cgttgttcgt
      361 ggcgccgtac ggcgagtcgc tgctgatcga cacgggcaac ggcggccaag cggccgcgcg
      421 cgacgcgggc cgaatcatgg cggccgtgca ggacgccgcg ctgacgcgca tcgatcacct
      481 gatcacgacg cactatcacg gcgatcactt cggcgccatg agcgagcttg cgggccgcat
      541 tccgatcggc gagttcatcg atcacggggc caacgtgcag cccaatcccg cgaccgacac
      601 gttcttgaac gacgtctacc cggcgctgta cgcgaagtcg aagcacaccg tcgtcaagcc
      661 gggcgaccgg gtcgcgatgc gcggcgtcga cgtccgcatc gtcgcgtcgg ccggcgaggc
      721 gattcgcgac gcggttccgg gcgcgggcca gccgaatccg cggtgcgcga gccatcagcc
      781 gaaggaccag gatccgagcg agaacgcgca atcggtcggc agccacttca cgttcggccg
      841 ctttcgcgcg ctgcatctcg gcgatctgac ttggaacaag gagttcgagc tcgcgtgccc
      901 gcgcaacctg ctcggcaccg tcgacctttt cgtcgtcacg catcacgggc agcccgcctc
      961 gaacgcgccc gtgctcgtgc acgcgctcgc gccgcgcgtc gcgatcatga acaacggcac
     1021 gcgtaaaggc gggcaacccg acaccatgaa agtgctgttc tcgtcgccgg ggctcgagga
     1081 tctctggcag ctgcacttct cgctgctgag cgggcaggaa gacacggtgc cgggcgcgtt
     1141 catcgcgaac gtcgccgacg aggagccggc gtcgcgcccg accgagccgg ccgcggctcc
     1201 ggcgcccggc acgaacgcgg cgccgccgcc cgcgcacaat ggcccggcgt tctggatcaa
     1261 ggtggccgcg cacgacgacg ggtccttcac cgtcacgaac ccgcgcaacg gcttcagcaa
     1321 gcattacgcg cgctgaatct tcaggcgccg cccccgaggc agttggcgct gacgcgcgcc
     1381 cgcaagtcac gcgcggcgga ggtttactcc ttccagcctt ccggcagctc cgttccggcc
     1441 ttgatctggc gcgtcttgta gcgccaatga ccggcgacct tgacgaaggt gctgtattcc
     1501 tttccttgcg tgatgacctt gacgggctcg ccttgcttct cgatcacgta ctcggtgaag
     1561 acgagctgcg cggtcgccgt gttgctgccg ccgtgcacga cgatgagcgg attgtctatg
     1621 gccacgatga acgagaagcg cggcggctgg gccggacgcg gcgcctgccc gggcgcgcgg
     1681 ccgtaggctt cgaggatgcc gttcctgccc ttgaagtggc gctcgccgag gatcagctcg
     1741 gcgtcctccg cataaaagtc agcgaagttc tcgccgttct cgcggccgaa gttgttgtag
     1801 tagcgcgtga tgagatcctg gatctgcagg cgatcgacga gcgtctcggc cgtgacttcc
     1861 tcggcgcgcg cgcccgagcc gatcgtcagt gcgagacagg cggcgcccaa ggcgccgagc
     1921 agtgctccgg agaatggacg tgcgaacgtt ttcattgtgg ttgccccccc tacttctgaa
     1981 gtgctccgcg aacgaagcat agactaagat acggcgacgc ggtcgaaaca ccaacgacga
     2041 ggagtccggc gatggcacca agactggtcg gttcccttgg caccctggca acgttggttg
     2101 gcgcgacgct tctctgctcg cagccgagcg caggtcagga acagagcgcc gctccgccgc
     2161 tcgtggtcac cgcattcggc gacaagccgg cgcctccgta cacggtcccg cgcacgtcgt
     2221 ggggcgaccc cgacttgcag ggcgcctggt cgagcgacga cacctccggc gttccgatgt
     2281 cgcggcgcga ggagcacggc gaccggctct atctcaccga cgaggagctt gcggagcgca
     2341 cgcagcaggt cgagcgcggc gtgcagcgcg ccgagaacga ggcgacgagc agcttccgcg
     2401 gcgacttcgc gcgccgcgcg ttgccgcaga cgtcgttgat cgtcgacccc ccgaacggtc
     2461 gcgtgcccgc ctacacggcc gaaggcatga agcggccgat gccgcgcggc acctacggca
     2521 acgggccgct cgacagctat accgatttct cgggctacga gcgctgcatc acgcgcggca
     2581 tcgtcggctc gacgctgcgc gtcgtttacg gcaacgggca gcgcatcgtc caggcgccag
     2641 gcgtcgtggc gctgtcgtac gagatggtgc acgacactcg cctcatctat accgacggcc
     2701 ggccgcacat cggccaagcg atacggcagt atctcggcga ttcgcgcgcg cactgggaag
     2761 gcgacgagct cgtcgtcgag acgacgaacc tcaccgatcg caccgcgatc
//
LOCUS       DASSNN010000234         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1559469, whole genome shotgun sequence.
ACCESSION   DASSNN010000234 DASSNN010000000
VERSION     DASSNN010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559469"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VFL84_04255"
     CDS             complement(<1..796)
                     /locus_tag="VFL84_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET7607866.1"
                     /translation="MAEGPLAGVRVLDLTRLLPGPMCTLYLADLGADVVKVEDTGAGD
                     YARTLSATPGATVAGTTAWYRALNRNKRSLAIDLKTASGHAAFMALARAADVIVEGFR
                     PGVVRQLRVDYETVRADNPRIVYCAVSGYGQTGPRAAAAGHDINYLGYAGTLEYTGAR
                     DGPPSLANLQIADLLGGAASAAIGILGALFGAARTGRGRYVDVAMADAVLAHQIFLLG
                     ALEEAGVVAPRGCDLLTGGVPCYGIYATRDGRFVAVGALEAKFWRTL"
     gene            complement(796..1980)
                     /locus_tag="VFL84_04260"
     CDS             complement(796..1980)
                     /locus_tag="VFL84_04260"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET7607867.1"
                     /translation="MTAAGPPAAIPPGSWLGLLGGGQLGRMFCMAAQRLGYRVAVVDP
                     AEDSPAGSVADRHLRADYLDPAALAALRESCAAATTEFENVPAASLEFLARDLRVAPG
                     AASVAIAQDRISEKTFLAGGGFAVAPFAVLRTRADAMTADEALLPGIVKRARFGYDGK
                     GQFRVRTRDDVTAAFDALLQEPAVLEQAVDLQAELSVVVARDDSGNVATWPISENRHR
                     DGILDVSIVPARIDAALADAALAIATGITTALDYRGVLCVELFVAPGNALLVNEMAPR
                     PHNSGHYTIDACATSQFEQQARVLAALPMGDTRQHTPAVMVNLLGDIWFDGDAAAPRE
                     PEWPRALSHGLAKLHLYGKREPRRGRKMGHVTCLGETLESALECAREIKRDLGIPGAD
                     AM"
BASE COUNT          302 a          713 c          702 g          286 t
ORIGIN      
        1 acagcgtgcg ccagaacttc gcttcgagag cccccacggc gacgaagcgg ccgtcgcgcg
       61 tcgcgtagat gccgtagcac ggaacgccgc ccgttagaag gtcgcatcca cgcggcgcga
      121 cgacgccggc ctcctcgagc gcgccgagca gaaagatctg atgcgcgagc acggcgtccg
      181 ccatggcgac gtcgacgtag cgcccgcgac ccgtgcgagc ggcgccgaac aaggcgccga
      241 gaatgccgat cgcggcggac gcggcgccgc ccagcaggtc ggcgatctgc aggtttgcga
      301 gcgacggcgg tccgtcgcgc gcaccggtgt actcgagtgt tcccgcgtag cccaggtagt
      361 tgatgtcatg gccggccgca gcggcgcgcg gaccggtctg cccgtatccc gacaccgcgc
      421 agtagacgat tcgcgggttg tcggcacgaa ccgtctcgta gtcgacgcgg agctgccgta
      481 cgacgccggg gcgaaagccc tcgacgatca cgtcggccgc tcgcgccagc gccatgaacg
      541 cggcgtgacc ggacgccgtc ttgaggtcga tcgcgagcga gcgcttgttg cgattgagcg
      601 cgcgatacca cgccgtcgtg ccggcgaccg tcgctcccgg cgtcgccgac agcgttcgcg
      661 cgtaatcgcc cgcgccggtg tcctcgacct tgacgacgtc ggcgccgaga tcggcgagat
      721 acagcgtgca catcggcccg ggcagaagcc gcgtcaggtc cagcacgcgc acgccggcga
      781 gcggcccttc cgcaatcaca ttgcgtccgc gcccgggatg ccgaggtcgc gcttgatctc
      841 gcgcgcacat tcgagcgccg attcgagcgt ttcaccgagg caggtcacgt gtcccatctt
      901 gcggccccgt cgcggctcgc gcttgccgta tagatgcagc ttggcgaggc cgtgcgaaag
      961 cgcccgcggc cactccggct cgcgcggcgc ggccgcgtcg ccgtcgaacc agatgtcgcc
     1021 cagcaggttg accatgacgg cgggcgtgtg ctggcgcgtg tcgcccatcg gaagcgcggc
     1081 caggacgcgc gcctgctgct cgaactgcga cgttgcgcac gcgtcgatcg tgtaatggcc
     1141 gctgttgtgc ggccgtggcg ccatttcgtt cacgagcagc gcgttgcccg gcgcgacgaa
     1201 cagttcgacg cacagcacgc cgcggtaatc gagcgcggtc gtgatcccgg tcgcgatcgc
     1261 gagcgccgca tcggccagtg ccgcgtcgat tcgcgccggc acgatcgaga cgtcgagaat
     1321 gccgtcgcga tggcggttct ccgaaatcgg ccaggtcgcc acgttgccgc tatcgtcgcg
     1381 cgcgacgaca accgagagct ccgcctgcag atcgaccgcc tgctcgagca ccgccggctc
     1441 ctgcaacagc gcgtcgaacg cggcggtgac gtcgtcacgc gtgcgcaccc ggaactgtcc
     1501 cttgccgtcg taaccgaagc gcgcgcgctt gacgatgcct ggaagcagtg cctcgtcggc
     1561 ggtcatggca tcggcgcggg tgcgcaggac ggcgaagggc gccacggcga atccgccgcc
     1621 ggcgaggaac gtcttctcgc tgatgcgatc ctgcgcgatc gcgacgctcg ccgcacccgg
     1681 cgcgacgcgc agatcgcgcg cgaggaattc gagcgaggct gcaggaacgt tctcgaattc
     1741 cgtcgttgcc gccgcgcacg actcgcgcaa cgccgcgagc gcggcgggat cgagatagtc
     1801 ggcccgcaga tgccggtcgg caacgctgcc tgccgggctg tcttcggcgg gatcgacgac
     1861 ggcgacgcgg tagccgagtc tctgcgcggc catgcagaac atgcgtccga gctggccgcc
     1921 gcccagcagg cccagccacg accccggcgg aatcgccgcg ggcggtccgg ccgcagtcat
     1981 gggacttccc tccgcgcttc gcg
//
LOCUS       DASSNN010000235         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1559559, whole genome shotgun sequence.
ACCESSION   DASSNN010000235 DASSNN010000000
VERSION     DASSNN010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559559"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFL84_04265"
     CDS             complement(<1..423)
                     /locus_tag="VFL84_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HET7607868.1"
                     /translation="MLRSDFHYELPDELIAQAPLPERRASRLLVLDGATGRIDDRLFA
                     DLPQLLRAGDLLVFNDTRVLPARVIGRKPTGGRVELLLERLLGPRSALVHLQASHKPA
                     PGSHVELPGGARARIGARRAELTEIELDRDVVAFLEAYG"
     gene            924..1325
                     /locus_tag="VFL84_04270"
     CDS             924..1325
                     /locus_tag="VFL84_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607869.1"
                     /translation="MSNWAIAAIIVNAALVVAVIVLALHTANRGVAVAVRVDVQVAST
                     VAAVTELVAAPSRPPLFSASQMTRAEWHALLNAYDEAHLKYLRALVAWNKGELRNYGT
                     LTRAHHDFEIAKRRLTRFELKASAGSPPYLS"
     gene            complement(1347..1733)
                     /locus_tag="VFL84_04275"
     CDS             complement(1347..1733)
                     /locus_tag="VFL84_04275"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7607870.1"
                     /translation="MEASSVRGVLFVKDLVKAAAFYVGALGLTRVSGDKDHAVLRCDA
                     FELVVQQIPREIAATIVIAEPPIRRVWGAIRLDFPVASVDESRRLARSLGGDVDAAPP
                     PWAERDANLFFGYDPEGNQFGVSRRK"
     gene            1883..2275
                     /locus_tag="VFL84_04280"
     CDS             1883..2275
                     /locus_tag="VFL84_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7607871.1"
                     /translation="MKFVLLIYNDPALLDALPKPEFDSRMRHCLEHADELKEHGKLLD
                     SQILGPPSAAKSVRIRGGRQTTTDGPFAETKEVIAGFNVIEADSIDEAVRMAAEFPWA
                     RLGCVEVRPMLDFDDVRKEVGAPSARRK"
BASE COUNT          395 a          814 c          789 g          414 t
ORIGIN      
        1 gccgtacgcc tcgagaaacg cgacgacgtc gcgatcgagc tcgatctcgg tgagctccgc
       61 cctgcgtgcg cctatgcgcg ccctcgcgcc gcccggcagc tcgacgtggc tgcccggcgc
      121 cggcttgtgg ctcgcctgga gatgcacgag cgcgctgcgc ggcccgagca atcgctcgag
      181 caggagctcg acgcgccccc cggtcggctt gcgcccgatc acgcgcgcgg gcagcacgcg
      241 cgtgtcgttg aagacgagca gatcgccggc gcgcaggagc tgcggcaaat ccgcgaacag
      301 ccgatcgtcg atgcggcccg tggcgccgtc gagcacgagc aagcggctcg cgcgccgctc
      361 gggaagcggc gcttgcgcga taagctcgtc gggaagctcg tagtggaagt cgctgcgcag
      421 catcggggcg cgattctagc ggcccgaggc gcgaagccgc gctttttctt gtctggtgcc
      481 cgaggtcgga gtcgaaccga cacgcttatt aggcggcgga ttttcgcgcc acttcggctt
      541 tcgccgcccg cgcaagccaa taaagccgcg aggttcgtgg tccggagcac gccttcacca
      601 tagccgttcg gccttaggtg cccgccgtct gctctctaca ccttccccgc tctttgagca
      661 gggcttggct cggcgtgagc tcgagctttc gctcagggcc ttcgccgagt ttgacgggct
      721 tcacctcgcg gatttctccg tgagggctca attcgtattg tctgagtccg ctgcgtctac
      781 cagttccgcc actcgggcaa gaaaaagctc cgctttgcgt cgcgcagtat agaagctgcg
      841 atggcggctc gtcacgcggc tctcgctatc gcggctcggg gcgcgtaggc tccgggacga
      901 tcggccccaa ggagggcgcg ccggtgagca actgggccat tgccgcgatc atcgtgaatg
      961 ccgcgctcgt cgtggcggtc atcgtgctcg cgctgcacac tgcgaatcgc ggcgtggcgg
     1021 tcgcggtccg cgtcgacgtg caggtcgcgt cgaccgttgc tgccgtgacg gagctcgtcg
     1081 ccgcgccttc gcggccgccg ctcttcagcg cttcgcagat gacgcgcgcc gaatggcacg
     1141 ccctgctgaa tgcgtacgac gaggctcatc tcaagtacct tcgagcgctg gtcgcttgga
     1201 acaaaggcga gctgcgaaat tacggcacgc tcacgcgcgc gcatcacgac ttcgagatcg
     1261 cgaagcggcg tttgacgcgc ttcgagctca aggcgagcgc cgggtcgcct ccttatctat
     1321 cgtgaggccg gcccgcgacg agaaccctac ttgcgtcgac tgacgccgaa ctgattgcct
     1381 tcggggtcgt agccgaaaaa caggttcgca tcgcgctcgg cccacggcgg cggcgccgcg
     1441 tcgacgtcgc cgccgagcga ccgcgcgagt cggcggctct cgtcgacgct cgccacgggg
     1501 aagtcgagcc ggatcgcgcc ccagactctc ctgatcggcg gttccgcgat cacgatcgtc
     1561 gccgcgatct cgcgcggaat ctgctgcacg acgagctcga acgcgtcgca tctcaagacg
     1621 gcgtgatcct tgtcgccgga tactcgagtc aggccgagcg cgccgacgta aaaggccgcc
     1681 gccttcacga gatctttgac gaacaggacg ccgcgtacgc tcgatgcttc caaatcgccc
     1741 ctccccaaaa aggggacaga ccccttttcc gagaaaatgg ggtctgtccc ctcacgcgtt
     1801 cgaaaggtag cacacgcgcc gtgacctatg gacacgctgc gggacgcccc agagaatcgc
     1861 cacatccgcg gagggcctag ccgtgaaatt cgtgctgctg atctataacg acccggcgct
     1921 gctcgatgcg ctgcctaaac ctgagttcga ctcgcgcatg cgtcactgcc tcgagcacgc
     1981 cgacgagctc aaggaacacg gcaagctgct cgactcgcag atcttggggc ctccttccgc
     2041 ggcgaaatcc gtgcggattc gcggcggccg tcagacgacg accgacgggc cgtttgccga
     2101 gacgaaggag gtcatcgccg gcttcaacgt gatcgaggcc gacagcatcg acgaggccgt
     2161 gcgcatggcg gccgagtttc cgtgggcgcg cttagggtgc gtcgaggtgc gcccgatgct
     2221 cgatttcgac gacgttcgca aagaggtcgg cgcgccgtca gctcgccgga aatagccacg
     2281 cgggcctgct cgtgtcgagc aggacgagcg cgagcaaccc gcccgcgatc gacacgttct
     2341 tccagcccgt cacgaactgc gggaagacga tcgcgccgtc tttcagcatg aacttgacgt
     2401 ggtagagcca cg
//
LOCUS       DASSNN010000236         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1665950, whole genome shotgun sequence.
ACCESSION   DASSNN010000236 DASSNN010000000
VERSION     DASSNN010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665950"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VFL84_04285"
     CDS             complement(<1..975)
                     /locus_tag="VFL84_04285"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET7607872.1"
                     /translation="MGELQVPASALWGAQTQRAVNNFPISGLRMPPAFIRALALIKWA
                     AARTNRNLGLLPEAVAAAIETSALEVANGMHADQFPIDVFQTGSGTSSNMNANEVIAR
                     LATRALGSPVHPNDHVNMSQSSNDSIPSAIHIAAALELKGKLEPALTHLAETIEAKAR
                     EVAGIVKTGRTHLMDAMPVRLDQELGGWAAQIRADIRRLQAVMPRLTGLAQGGTAVGT
                     GINAHPKFSAEFAAVLAKQTGVDFHPAEDFFEALSSQDTAVELSGQLKTTAVSIMKIA
                     NDLRWMNSGPLAGLSEIHLPDLQPGSSIMPGKVNPVIPEAAAMVAAQVI"
     gene            1099..>2481
                     /gene="purB"
                     /locus_tag="VFL84_04290"
     CDS             1099..>2481
                     /gene="purB"
                     /locus_tag="VFL84_04290"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET7607873.1"
                     /translation="MDATSLLALSPLDGRYARKAAPLREYLSEYALIRYRTLIEVRWL
                     QHLADEPSIAELESLDPPIKDVLNHIVDEFSVDDALRIKALEAKTNHDVKAVEYFLRQ
                     KLEGVAVTSGNLSSFIHFACTSEDINNLSYALMLRDARQRVLLPAMRELTTDLRELAH
                     RFAAVPMLARTHGQPATPTTLGKEIANFVYRLQRQTELFAAVAIHGKLNGAVGNLNAH
                     VAAYPNADWLAITDRFLDSLGVQPTRYTTQIEPHDWIAEYCQALSRFDTVLVDLCRDF
                     WSYISLGYFRSRPVAGEVGSSTMPHKVNPIDFENAEGNLGLANELLGFLATKLPVSRW
                     QRDLTDSTVLRNVGVALGHALIALEAASKGLRKLDADLDAIAADLANRWEVLAEAVQT
                     VMRRYGLPDAYEQLKDLTRGKAIDRDALHAFIRAAALPADARDRLLALTPRGYVGEAA
                     ELARSAADAIE"
BASE COUNT          403 a          862 c          787 g          429 t
ORIGIN      
        1 gatgacttgg gccgcgacca tcgcggcggc ttccggaatc acggggttga ccttgcccgg
       61 catgatgctg ctgcccggct gcaaatccgg caggtggatc tccgacaagc cggccagcgg
      121 cccgctgttc atccagcgta ggtcgttggc gattttcatg atgctgacgg ccgtcgtctt
      181 cagctgcccc gacagctcga cggccgtgtc ctggctgctc agcgcctcga agaaatcctc
      241 ggccggatga aaatcgacgc ccgtctgctt cgcgagcacg gccgcgaact ccgcgctgaa
      301 cttcggatgc gcattgatgc ccgtgccgac ggccgtgccg ccctgcgcga gccccgtgag
      361 ccgcggcatg acggcctgca agcggcgaat gtcggcccgg atttgcgccg cccagccgcc
      421 gagctcttga tcgaggcgca ccggcatcgc gtccatcaag tgggtacgcc ccgtcttgac
      481 gatgccggcg acctcgcgcg ctttcgcttc gatcgtctcc gcgaggtgcg tgagcgccgg
      541 ctcgagcttg cccttgagct cgagcgcggc cgcgatgtgg atcgccgacg gaatcgagtc
      601 gttactgctc tggctcatgt tgacgtgatc gttcggatgc acgggcgacc cgagcgcccg
      661 cgtcgcgagc cgcgcgatga cctcgttcgc gttcatgttc gagctcgtgc ccgagcccgt
      721 ttgaaacacg tcgatcggaa actgatccgc atgcatgccg ttcgcgacct cgagcgcgct
      781 cgtctcgatc gccgcggcga cggcttccgg cagcaagccc aagttacggt tcgtgcgcgc
      841 ggcggcccac ttgatcagcg ccaatgcgcg aatgaacgcc ggcggcattc gtaggccgct
      901 gatcgggaaa ttgttgaccg cgcgttgcgt ctgtgcgccc catagcgcgg atgcgggcac
      961 ctgcagttcg cccatgctgt cgcgctccac acgaaaatcg gccggcatgc atactcctcg
     1021 agacctaagc ccgcacggcg cggactcgcg gtgtatgatg cgcactccga aaaggcttgt
     1081 ataggcttcg gccggacgtt ggacgcaacc tcgctgctcg cactttcgcc gctcgacggc
     1141 cgctacgcgc gcaaggccgc gcccctgcga gagtaccttt cggaatacgc gctgatccgc
     1201 taccgcacgc tgatcgaagt gcgctggctg cagcatctcg ccgacgagcc gagcatcgcc
     1261 gagctcgaat cgctcgaccc gccgatcaag gacgtgctga accacatcgt cgacgagttc
     1321 agcgtcgacg acgcgcttcg catcaaagcg ctcgaagcca aaacgaatca cgacgtcaaa
     1381 gccgtcgagt atttcttgcg gcagaagctc gaaggcgtcg ccgttacatc gggcaacctc
     1441 tcctcgttca ttcatttcgc gtgcacctcg gaggacatca acaatttgtc ctacgcgctg
     1501 atgctgcgcg acgcgcgcca gcgcgtgctg ctgccggcga tgcgcgagct gacgacggat
     1561 ctgcgcgagc tcgcgcaccg gttcgcggcc gtgccgatgc tggcgcgcac gcacggccag
     1621 cccgccacgc cgacgacgct cggcaaggag atcgcgaact tcgtataccg gctgcagcgg
     1681 cagaccgagc tctttgcggc cgtcgcgatt cacggcaagc tcaacggcgc ggtcggcaat
     1741 ttgaacgcgc acgtcgccgc atacccgaac gcggattggc tcgcgatcac ggaccgtttc
     1801 ctcgattcgc tgggcgtgca gccgacgcgc tacacgacgc agatcgagcc gcacgactgg
     1861 atcgccgagt actgtcaggc gttgtcgcgc ttcgacacgg tgctcgtcga tctctgccgc
     1921 gacttctgga gctacatcag cttgggatac ttccgctcgc ggcccgtcgc cggagaggtc
     1981 ggctcgtcga cgatgccgca caaggtcaac ccgatcgact tcgagaacgc cgagggcaac
     2041 ttaggacttg cgaacgagct gctcggtttc ctggcgacga agctgccggt ctcgcgctgg
     2101 cagcgcgacc tcacggattc gacggtgctg cgcaacgtcg gcgtcgcgct cgggcacgca
     2161 ttgatcgcgc tcgaggccgc gtcgaagggc ctccgcaagc tcgatgcgga tctcgacgcg
     2221 atcgccgccg atctcgccaa ccgctgggag gtgctggccg aagccgttca aacggtcatg
     2281 cgccgctacg gcttacccga cgcctacgag cagctcaagg acctgacgcg cggcaaggcg
     2341 atcgaccgcg acgcgctgca cgcgttcatc cgcgccgccg cgcttcccgc cgacgcgcgc
     2401 gatcgcctcc tcgcgctgac gccgcgcggc tacgtcggcg aggcggccga gctcgcgcgc
     2461 tccgccgccg acgcgatcga g
//
LOCUS       DASSNN010000237         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1430895, whole genome shotgun sequence.
ACCESSION   DASSNN010000237 DASSNN010000000
VERSION     DASSNN010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430895"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1350)
                     /gene="kynU"
                     /locus_tag="VFL84_04295"
     CDS             complement(25..1350)
                     /gene="kynU"
                     /locus_tag="VFL84_04295"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HET7607874.1"
                     /translation="MAAPKFSPGLAGARALDADDELASLRREFELPRERGRTLIYLCG
                     HSLGLMPRGARRAVEAELDRWAALGVDGHFPDAAPKRGRARPLRDAHGGWVDYHVRFA
                     PLLADLVGAGRDEVVAMNTLTVNLHLMLATFFRPTAQRFKILIERDAFSSDRYAVESQ
                     LRWHGLDPRAALLELGFRDGTFELDRARYEALLAEHGESIALVLLPGVQYLTGERLDL
                     AAYAAAARRYGCRVGFDLAHAIGNVPLAIHDSGADFAVWCSYKYLNAGPGAVAGCFVH
                     RRHGEAENLPRLAGWWGHDRGRRFLMEPKFTPLSGAEGWQISNPPILSLAPLSASLAL
                     FDEAGMKRLRRKSLTLTRYLEWLLDRELGGRVRLLTPTDPERRGCHVAMRFVPPPADA
                     SAFVARLRAAGVVADWRKENVLRLAPVPLYNRYRDVYGAVQALKRTLDQ"
     gene            complement(1353..1865)
                     /locus_tag="VFL84_04300"
     CDS             complement(1353..1865)
                     /locus_tag="VFL84_04300"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HET7607875.1"
                     /translation="MKTTMPFKLLDWIDDNREHLRPPVCNREMFPAGDFIVQVVGGPN
                     ARTDYHLDEGPELFYQVEGQMLLKTVQDGQFVDIPIEAGDIFLLPPRIPHSPQRMAKS
                     VGIVVERARAPHELDGFMWFCPSCANKLHEEYLHVADIVKDLPPVFERFYGSVEARTC
                     KRCGTVMPPK"
     gene            complement(1948..>2159)
                     /locus_tag="VFL84_04305"
     CDS             complement(1948..>2159)
                     /locus_tag="VFL84_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HET7607876.1"
                     /translation="HAAPPFEVRDRAKFAALVAHLFSMRRKTLGRALKGRLTPEAIAS
                     VGVDPKARPETLAPADFARLAALES"
BASE COUNT          343 a          737 c          773 g          306 t
ORIGIN      
        1 cgatgcgctg cttcgccggc gcgctcattg gtcgagcgtg cgcttgagcg cctgcacggc
       61 tccgtagacg tcgcgatagc ggttgtagag cggcaccggt gccaagcgca gcacgttctc
      121 tttgcgccaa tccgcgacga cgcccgcggc gcgcagtcgc gcgacgaacg ccgacgcgtc
      181 ggccggaggc ggcacgaacc gcatcgcgac gtgacagcct cgccgctcgg gatccgtcgg
      241 ggtcagaagg cgcacgcgtc cgccgagctc gcggtcgagc agccattcga ggtagcgcgt
      301 cagtgtcagc gacttgcgcc gcaagcgctt catgccggcc tcgtcgaaca gcgcgagcga
      361 cgccgacagc ggcgcgagcg acaggatcgg aggattgctg atctgccagc cttcggcgcc
      421 cgacagcggc gtgaacttcg gctccatgag gaagcgccgg ccgcgatcgt ggccccacca
      481 gcccgcgagc cgcggcaggt tctcggcctc gccgtgacgc cgatgcacga agcagcccgc
      541 gacggcgccg ggacccgcgt tcaaatattt gtagctgcac cagacggcga agtcggcgcc
      601 cgagtcgtgg atcgcaagcg gcacgttgcc gatcgcgtgc gcgaggtcga agccgacgcg
      661 gcagccgtaa cgccgcgcgg ccgcagcgta cgcggcgaga tcgagccgct cgccggtcag
      721 atactgcacg ccgggcagca gcacgagcgc gatgctctcg ccgtgctctg cgagcagcgc
      781 ctcgtagcgc gcccggtcga gctcgaaggt gccgtcgcgg aacccgagct cgaggagcgc
      841 ggcgcgagga tcgagcccgt gccagcgcag ctgcgattcc acggcgtagc ggtccgacga
      901 gaacgcgtcg cgctcgatca ggatcttgaa gcgttgcgcc gtgggcctga agaatgtcgc
      961 gagcatcagg tgcaggttca ccgtcagcgt gttcatcgcg acgacttcgt cgcggccggc
     1021 gccgacgaga tcggcgagca gcggcgcgaa ccgcacgtga tagtcgaccc aaccgccgtg
     1081 cgcgtcgcgc aatggccgcg cgcggccgcg tttcggagcc gcgtccggaa aatgcccgtc
     1141 gacgccgagc gcggcccacc ggtcgagctc cgcttcgacg gcgcggcgcg cgccgcgcgg
     1201 catcaagccg agcgaatggc cgcacaggta gatcagcgtg cggccgcgct cgcgcggcag
     1261 ctcgaactcg cgccgcagcg aagcgagctc gtcgtcggca tcgagcgcgc gcgcaccggc
     1321 gagcccgggt gaaaacttcg gcgcggccac ggctacttgg gcggcatcac ggtgccgcag
     1381 cgcttgcacg tgcgcgcctc gacgctgccg tagaagcgct cgaacaccgg cggtaggtcc
     1441 ttgacgatgt cggcgacgtg caggtactcc tcgtgcagct tgttcgcgca gctcgggcag
     1501 aaccacatga agccgtcgag ctcgtgcggc gctcgcgccc gctcgacgac gatgccgacc
     1561 gacttcgcca tgcgctgcgg cgaatgcggg atgcgcggcg gcaacagaaa gatgtcgccc
     1621 gcctcgatcg ggatgtcgac gaattggccg tcctgcaccg tcttcaacag catctggccc
     1681 tcgacctggt agaagagctc ggggccttcg tccaagtgat agtccgtgcg cgcgttcggg
     1741 ccgccgacga cttgcacgat gaagtcgccg gccggaaaca tctctctgtt gcagacgggc
     1801 gggcgcaggt gctcgcggtt gtcgtcgatc cagtcgagca gcttgaacgg catcgtcgtc
     1861 ttcattgcaa atccctcgcg cggatgcggc gcgccgcggc gccgtgcacg cattatgccg
     1921 cgaggcgggg gcggtcggcg cgataagtca ggattcgagc gccgcgagcc gcgcgaaatc
     1981 ggccggcgcg agagtctcgg ggcgagcttt cgggtctaca cctacggacg cgatcgcctc
     2041 gggcgtcagc cggcccttca gcgcgcggcc gagcgtcttg cggcgcatcg agaagagatg
     2101 cgcgacgagc gcggcgaact tcgcgcggtc gcgcacctcg aatggcggag cggcgtgcg
//
LOCUS       DASSNN010000238         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1793404, whole genome shotgun sequence.
ACCESSION   DASSNN010000238 DASSNN010000000
VERSION     DASSNN010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793404"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFL84_04310"
     CDS             <1..348
                     /locus_tag="VFL84_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607877.1"
                     /translation="VGDGEYVSLNELVKLIGKVLGTRVKLYRFPWQKPVHGLAYLMEV
                     ASRPFDYEPPLFRRRLTWFSTNRGWEITRARTELGFRPRISLEQGLALTKNWYYERGL
                     LSLAFLSTFPLAP"
     gene            complement(373..1389)
                     /locus_tag="VFL84_04315"
     CDS             complement(373..1389)
                     /locus_tag="VFL84_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET7607878.1"
                     /translation="MSQDSIIRVAPLAFPWQTKDPFLVCVHHVDHYPRGNDRLGPAAS
                     LDGRDIGQDFAGKDGWRMYHGNVVPGFPQHPHRGFETVTFVREGFIDHSDSLGATARF
                     GRGDVQWLTAGAGIVHSEMFPLLKPDASNRLELFQIWVNLPAKSKFAAPYFTMFWDKD
                     VPRVRPAEGVELTLVAGALDGAQPPAPPPDSWAANPEAELAIWLITLEQGAKWTLPAA
                     RNAQTARTLYFFSGSNVEIEGKALPKHAAVEVAANRPIGLAAPDGTVEVLLMQGRPIG
                     EPVAQYGPFVMNTRAELEQAFYDYQRTQFGGWPFKANDPVHPRERGRFARHADGRVEE
                     AAPP"
     gene            complement(1386..>3340)
                     /locus_tag="VFL84_04320"
     CDS             complement(1386..>3340)
                     /locus_tag="VFL84_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET7607879.1"
                     /translation="AWVEAQNAVTFPYLERIANRDALRARVMELNDYERYGAPARKGP
                     YFFFNKNEGLQNQSVLYMQQGLDGAPEVLLDPNSWSADGTVQLSAFEPSKDAKYAVYG
                     ISQSGSDWIQYKVMELATKRTLADTVDWAKVSSVAWAGDGFYYSRYPEPPEGHEKASI
                     NENHQVFFHKLGTPQSADTLVFEDPSNPQRFHTTTTTEDERFLILEVSDRGKGLDGNA
                     LYVRDLKDPTAKFSPLVPEITNDTFYVVDNVGDQILVATNHGAPNWRVVQIDPKNIAE
                     ANWKTILAERPEPIDSVNMAGGKLFATYLKDVATRAYVFGLDGKLENEIALPGIGTAG
                     GFGGPNDSPFVFYTFNSLSVPPTIYRYDIATRASSVFRAPRVPGYDASQYETKQVFYP
                     SKDGTRIPMFLVYRKGLNLDGNNPTLLYGYGGFNIVVSPTFSAARIALLEQGFVYASA
                     NLRGGAEYGEAWHRQGMKLNKQNVFDDFIAAAEWLIKEKYTSTPKLAITGRSNGGLLV
                     GAVLNQRPDLFGAAVPEVGVMDMLRFDKFTIGWNWIADYGSSDDAEEFKALYAYSPLH
                     NIKAGAKNPPTLVTTADHDDRVVPAHSFKYAATLQALASKENPVLIRIETKSGHGASS
                     LTKALEATGDIYAFLMHTLGVEPKKP"
BASE COUNT          524 a         1070 c         1119 g          627 t
ORIGIN      
        1 gtcggcgacg gcgaatacgt atctttgaac gagctggtca agctcatcgg caaggtgctc
       61 ggaacccgag tgaagctgta ccgattcccg tggcagaagc ccgtgcacgg cctcgcttat
      121 ctcatggaag tcgcgagcag gcccttcgat tacgagccgc cgttgttccg ccgaagactg
      181 acgtggttca gcacgaaccg cggttgggag atcacgcgcg cacgcacgga gctcggcttt
      241 cggccgcgga taagtctcga gcaaggactc gcgctcacga agaactggta ttacgagcgc
      301 gggctgctct ccttggcttt tctgtcgacc tttccgcttg cgccctgagc gcacgcgctc
      361 ccgcgctgaa gcttacggag gcgccgcttc ctcgacgcgc ccgtcggcgt ggcgcgcgaa
      421 ccggccgcgt tcgcgcggat gcacgggatc gttcgccttg aacggccagc cgccgaattg
      481 cgtgcgttgg tagtcgtaga acgcctgctc gagctctgcg cgcgtgttca tgacgaacgg
      541 cccgtactga gcgacgggct cgccgatcgg gcgcccctgc atcagcaaca cctcgaccgt
      601 gccgtcgggg gccgcgagcc cgatgggccg attcgctgcg acctcgaccg cggcatgctt
      661 cggcagcgct ttgccctcga tctcgacgtt gctgcccgag aagaagtaca gcgttcgcgc
      721 cgtttgcgcg ttcctagcgg cgggtaacgt ccacttcgcg ccctgctcga gcgtgatgag
      781 ccagatcgcg agctcggctt ccggattcgc ggcccacgag tcgggcggcg gcgccggcgg
      841 ctgcgcgccg tcgagcgcgc cggcgacgag cgtgagctcg acaccctcgg caggtcgaac
      901 gcgcggcacg tccttgtccc agaacatcgt gaagtacggc gccgcgaact tcgatttcgc
      961 cggcaagttg acccagatct gaaacagctc gagccgattg gacgcgtcgg gcttcaacag
     1021 cgggaacatc tccgagtgca cgatgccggc gccggccgtg agccactgca cgtcgccgcg
     1081 cccgaagcgc gccgtcgcgc cgagcgagtc gctgtgatcg atgaagcctt cgcgcacgaa
     1141 cgtcacggtc tcgaagccgc ggtgcgggtg ctgcggaaaa cccggcacga cgttgccgtg
     1201 atacatgcgc cagccgtcct tgccggcgaa gtcctggccg atgtcgcggc cgtcgagcga
     1261 cgccgcgggg cccaggcggt cgttgccgcg cggatagtgg tccacgtggt gcacgcagac
     1321 gaggaacggg tccttcgttt gccacgggaa tgcgagcggc gcgacgcgga tgatcgagtc
     1381 ctggctcatg gcttcttcgg ctccacgccg agggtgtgca tcaggaacgc gtagatgtcc
     1441 ccggtcgcct cgagcgcctt cgtcaagctg ctcgcgccgt gcccggactt cgtctcgatg
     1501 cggatcagca cggggttttc cttgctcgcg agcgcctgaa gcgtcgccgc gtacttgaac
     1561 gaatgcgcgg gcacgacgcg gtcgtcgtgg tcggccgtcg tgacgagcgt gggcggattc
     1621 ttcgcgccgg ccttgatgtt gtgcaatggc gaatacgcat acagcgcttt gaactcttcc
     1681 gcgtcgtcgc tcgagccgta gtccgcgatc cagttccagc cgatcgtgaa cttgtcgaag
     1741 cgcagcatgt ccatgacgcc gacctcggga acggccgcgc cgaacagatc ggggcgctgg
     1801 ttcaagaccg cgccgacgag caggccgccg ttcgagcggc ccgtgatcgc gagcttcggc
     1861 gtcgacgtgt acttctcctt gatgagccac tcggccgccg cgatgaaatc gtcgaacacg
     1921 ttctgcttat tgagcttcat gccctggcgg tgccacgcct cgccgtactc ggcgccgccg
     1981 cgcaagttgg ccgacgcgta gacgaagccc tgctcgagca gcgcgatgcg cgcggcgctg
     2041 aacgtcgggc tcacgacgat gttgaagccg ccgtagccgt agagcagcgt cggattgttg
     2101 ccgtcgaggt tcagtccctt gcggtagacg aggaacatcg ggatgcgcgt gccatccttg
     2161 ctcggataga agacctgctt cgtctcgtac tgcgacgcgt cgtaacccgg cacgcgcggc
     2221 gcgcggaaga cgctgctcgc gcgtgttgcg atgtcgtagc gatagatcgt cggcggcacg
     2281 ctcagcgaat tgaaggtgta gaacacgaac ggcgagtcgt tcggcccgcc gaaaccgccg
     2341 gccgtgccga tgccgggcag cgcgatctcg ttctcgagct tgccgtcgag gccgaacacg
     2401 taggcgcgcg tcgcgacgtc cttcaggtac gtcgcgaaca gcttgccgcc cgccatgttg
     2461 acgctgtcga tcggctcggg gcgctcggcg aggattgttt tccaattcgc ctcggcgatg
     2521 ttctttgggt cgatctgcac gacgcgccag ttcggcgcgc cgtgattcgt cgcgaccagg
     2581 atctgatccc cgacgttgtc gacgacatag aacgtgtcgt tcgtgatctc cggcacgagc
     2641 ggcgaaaact tggccgtggg atctttgaga tcgcgcacgt agagcgcgtt gccgtcgagg
     2701 cctttgccgc gatccgacac ctcgaggatc aggaagcgct cgtcttcggt cgtcgtcgtc
     2761 gtgtgaaagc gctgcggatt gctgggatcc tcgaacacga gcgtgtcggc cgactgcggc
     2821 gtgccgagct tgtggaagaa gacctgatgg ttctcgttga tcgacgcctt ctcgtggcct
     2881 tcgggcggct cgggatagcg gctgtagtag aagccgtcgc cagcccaagc gacgctcgag
     2941 accttcgccc aatcgacggt gtccgcgagc gtgcgcttcg tcgcgagctc catcactttg
     3001 tattggatcc aatcggagcc gctctgcgag atgccgtaga cggcgtactt cgcgtccttc
     3061 gacggctcga aggccgagag ttgcacggtt ccgtcggcgg accacgagtt cgggtcgagc
     3121 agcacctcgg gagcgccgtc gagaccctgc tgcatgtaca gcacgctttg gttttgcagg
     3181 ccctcgttct tgttgaagaa gaagtacggg cccttgcgcg ccggcgcgcc gtagcgctcg
     3241 tagtcgttga gctccatgac gcgcgcgcgc agcgcgtcgc ggttggcgat gcgctcgaga
     3301 tacgggaacg tcacggcgtt ttgcgcctcg acccacgcgg
//
LOCUS       DASSNN010000239         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1055666, whole genome shotgun sequence.
ACCESSION   DASSNN010000239 DASSNN010000000
VERSION     DASSNN010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055666"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFL84_04325"
     CDS             complement(<1..415)
                     /locus_tag="VFL84_04325"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961211.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET7607880.1"
                     /translation="MDKLAIVGGNPLDGEVRISGAKNAALPILAATLLAEGAVTLRNV
                     PKLNDIATTLKLLKRMGVAAEHREDGSVTVDAGGIKEFIAPYELVKTMRASILVLGPL
                     LGRFGHADVSLPGGCAIGARPVNLHVMGLRAMGAEI"
     gene            complement(425..673)
                     /locus_tag="VFL84_04330"
     CDS             complement(425..673)
                     /locus_tag="VFL84_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HET7607881.1"
                     /translation="MDPRDIAALIEQNLPGSKAHVQTDGQGHYEATVISDSFAGKRSL
                     QRHQLVYGTLGSRVGREIHALAIQALTPDEWRARSPSG"
     gene            804..1361
                     /gene="lptC"
                     /locus_tag="VFL84_04335"
     CDS             804..1361
                     /gene="lptC"
                     /locus_tag="VFL84_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HET7607882.1"
                     /translation="MRNTIVMVVLAIVAAATWILTWQRQDEAPPVERIGDAEPLGYYA
                     RGARISGTDEQGRLSYRIFADRLDELPGEELLELTGVKVDYQPADETAWTLTAASGTY
                     AWDGSLLDLNGDVEARSMPADGSRALTMTTQKMVFSPDTSSAASDGEVAIRVGDWQLS
                     AVGLRADLKGDTLALESEVHGTILP"
     gene            1342..1968
                     /locus_tag="VFL84_04340"
     CDS             1342..1968
                     /locus_tag="VFL84_04340"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HET7607883.1"
                     /translation="MARFFPSTLRGPAVAFAVALAAGAQDNNAADELQFSSDTLVLDG
                     QSNMMRAQQPRITQGNLRIAADDALATGFEFDEAGEFRLTGNVRVDVDTASMQASSAV
                     FTYANGQLSRGELEGSPVRFSEVDPATQKKVTGHAGKMSFDNVARTLRMTGDATVQLD
                     SREVLGCDLIYDFRAERVTSGSADCEGGFRVRVRRDSEERAAAPDPPQ"
     gene            1965..>2084
                     /locus_tag="VFL84_04345"
     CDS             1965..>2084
                     /locus_tag="VFL84_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HET7607884.1"
                     /translation="MSRLEALGVAKRYRTRQVVKDLSMVVDSGEVVGLLGPNGA"
BASE COUNT          355 a          697 c          691 g          341 t
ORIGIN      
        1 tgatctcggc gcccatcgcc cgcaggccca tgacgtgcag gttcacgggc cgcgcgccga
       61 tcgcacagcc gcccggcaac gagacgtcgg cgtgcccgaa ccgcccgagc agcggcccga
      121 gcacgaggat cgacgcgcgc atcgtcttga cgagctcgta gggggcgatg aattccttga
      181 tgccgcccgc gtcgacggtc acgctgccgt cctcgcgatg ctcggccgcg actcccatgc
      241 gcttgagcag cttcagcgtc gtcgcgatgt cgttgagctt cgggacgttg cgaagcgtca
      301 ctgctccttc ggcgagcagc gtcgcagcga gaatcggcaa cgccgcgttt ttcgcgcccg
      361 agattcgcac ctcgccgtcg agcgggttac cgccaacgat cgctagcttg tccatgcggc
      421 gtcttcagcc cgacggcgag cgagcgcgcc actcgtcggg cgtcagcgct tgaatcgcga
      481 gcgcgtggat ctcgcgcccg acgcgcgagc cgagcgtgcc gtacacgagc tgatgccgct
      541 gcaggctgcg ttttccggcg aacgagtccg agatcacggt ggcttcgtag tgaccttggc
      601 cgtccgtttg gacgtgcgct ttcgaaccgg gaaggttctg ctcgatcaat gcggcgatat
      661 cgcgcggatc catgatgagg gcatcgagtg gctcgagccc gtagtctagc ccatcgcgcg
      721 cgaccgatgc gccgggctcc attcccaact ctcgcgtaca atcgtgcgtc ggcggagcac
      781 cgcctatcgt caaggtcttc cggttgcgca acaccatcgt catggtcgtg ctcgcgatcg
      841 tcgcagccgc cacgtggatc ctgacgtggc aacggcaaga cgaggccccg cccgtcgaac
      901 gcatcggcga cgccgaaccg ctcggctact acgcgcgcgg agcgcgcatc tcgggcaccg
      961 acgagcaagg ccggctgtcg taccgcatct tcgccgaccg gctcgacgag ctgcccggcg
     1021 aggagcttct cgagctcacc ggcgtcaagg tcgattacca gcccgccgac gaaacggcgt
     1081 ggactctgac ggccgcgagc ggcacgtacg cgtgggacgg ctcgcttctc gatctgaacg
     1141 gcgacgtcga ggctcgaagc atgccggccg acggctcgcg cgcattgacg atgacgacgc
     1201 agaagatggt gttttcgccc gacacttcga gcgccgcatc ggacggagaa gtcgcgatcc
     1261 gtgtcgggga ctggcaactc agtgccgtcg gcctccgcgc ggatttgaag ggggatactc
     1321 tggcgctaga atcggaggtg catggcacga ttcttcccta gcaccctgcg cggacctgcc
     1381 gtcgcgttcg cagtcgccct cgcggccggc gcacaagaca acaatgccgc cgacgagctg
     1441 caattcagct ccgacacgct ggtgctcgac gggcagtcga acatgatgcg cgcgcaacag
     1501 ccgcgcatta ctcaaggcaa tctgcgcata gcggccgacg atgcgctcgc gaccggcttt
     1561 gagttcgacg aagccggcga gtttcggctc accggcaacg ttcgcgtcga cgtggatacg
     1621 gcgtcgatgc aagcgagctc ggccgtcttc acctatgcga acggtcaact atcgcgcggc
     1681 gagctcgaag gctcacccgt acgcttcagc gaggtcgacc cggcgacgca aaaaaaagtg
     1741 acgggacacg ccggcaagat gtcgttcgac aacgtcgcgc gcacgctgcg catgaccggc
     1801 gacgcgaccg tgcagctcga cagccgcgag gttctcggct gcgacctcat ctacgacttt
     1861 cgcgccgagc gcgtgacgtc cgggtcggcg gattgcgagg gcggttttcg ggtcagggtt
     1921 cgccgcgatt ccgaagaacg agccgccgct cccgatccgc cccaatgagc aggctggagg
     1981 cgctcggagt cgccaagcgc taccggacca ggcaggtcgt caaggacttg agcatggtcg
     2041 tagacagcgg cgaggtcgtg gggctgctcg gcccgaacgg cgcg
//
LOCUS       DASSNN010000240         3435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_44644, whole genome shotgun sequence.
ACCESSION   DASSNN010000240 DASSNN010000000
VERSION     DASSNN010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44644"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1497
                     /locus_tag="VFL84_04350"
     CDS             313..1497
                     /locus_tag="VFL84_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607885.1"
                     /translation="MRLKTLSSTIAALLLLSASAMVLAQEGEGPPPARGGRGGGGGPG
                     GQAARPPTAEEIAALPADRRIPAEKQTALAKNPSWKAPRTSWGDPAIAGTYSTDDMRG
                     IPRDRPQALATQESLPEEQFLARAVQQYSGATRAATTETFLRNEWGTRTFGFTSLVVD
                     PPDGRTPAVTEAARERGRAGNSSFSNDRFDSFEDFSLYDRCIGRGISNGMNAAIYGNG
                     IQVFQSPGVVTITYEMIHETRVIKLDNSPYPEIAQFTGNARGHWEGDTLVIESRGFQD
                     RTSVGGAPNSANLRTTERIRRVDPEMLEYRITINDPETYTAPFTVRTMWTTQPDYYAY
                     EYSCHEGNFAVGGGLAGERALDREKAAAIAAGKPLPERSRANIYGRPEEGAEIFDINA
                     GE"
     gene            complement(1666..2541)
                     /locus_tag="VFL84_04355"
     CDS             complement(1666..2541)
                     /locus_tag="VFL84_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607886.1"
                     /translation="MSEQRAAERPEILHTPDADASGFAVAAAKLGFWSRLYGNGAVRK
                     SVLLALLAAAWEIYARALGNPLLFPPFTATVGALLSGIARGEIPAAGLYTLSLLLKGY
                     VAGLFFAAVLTAFASVSRIGADLLETLTAMFNPLPSIALLPLALIWFGLGDSSVIFVL
                     VHAVLWAVALNTFAGFRALSSTLRMVGQNYGLSSAGYIARILIPGAFPSILTGLKVGW
                     AFAWRTLIAAELVFGVSSGSGGLGWYIFENKNQLRIPEVFAGLLTVIIFGLFVENVVF
                     RTIEHRTVRRWGMQI"
     gene            complement(2534..3307)
                     /locus_tag="VFL84_04360"
     CDS             complement(2534..3307)
                     /locus_tag="VFL84_04360"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7607887.1"
                     /translation="MREPALLSVKGVTLQYRTGQQLVTATYRVSFDVLKGDRFVVLGP
                     SGCGKSTLLKAIGGYLSPVEGEIVLNGARVGRPGPDRAFVFQEFDQLLPWKTVCDNVV
                     FALTASGKLAKKAATERALHFIAKVGLTKFADSFPHTLSGGMKQRVAIARAMAMEPQI
                     LLMDEPFAALDALTRGKMQEDLLELWNEARFTVLFVTHSIPEAIRIGNRILLLTPHPG
                     RTKAEINCEGVDVAGAGGELTSARIQRLLFDRTGAIADV"
BASE COUNT          636 a         1148 c         1157 g          494 t
ORIGIN      
        1 cgacggcaaa cgccatccag acgccggaca aatccggtac cgcgggccgc tcggccgcgg
       61 ccgatgcggc cggtgcgcct gccggaggcg tgcaagacgt catgagcgcc ggcgcagtca
      121 aacaagcggc gatacgcaga atgatcccgg tgcttcgcat gacggcggct gtcccaagcg
      181 gctggtcgcc gaatcgtact tgaagaagtc tggtttcgcc gccccgggta gttcccaccg
      241 ccgcggtggc gtgcttgaat ctgcgtaccg cggtcggggg actcgcggcg caagcccttc
      301 gaggagcatc acatgagact gaagacgttg tcgtcgacga tcgcggccct gcttttgctt
      361 tccgcatccg cgatggtgct cgcgcaggag ggcgagggac cgccgcccgc gcgcggcgga
      421 agagggggtg gcggcggacc gggaggccag gccgcgcgac cgccgacggc ggaagagatt
      481 gctgcgctgc ccgcagaccg gcgcataccc gccgagaaac agacggcgct cgccaagaat
      541 ccgagctgga aggccccgcg cacgtcgtgg ggcgatccgg cgatcgcagg cacatacagc
      601 acggacgaca tgcgcggcat cccgcgcgat cggccgcaag cgctcgccac gcaggagtcg
      661 ttgcccgaag agcaattcct cgcacgcgcc gtgcaacagt actcgggcgc aacgcgcgcc
      721 gcgacgaccg agacgttctt gcgcaacgag tggggcacgc gaacgttcgg cttcacctcg
      781 ctggtcgtcg atcccccgga tggccgcacg ccggccgtca ccgaagcggc tcgcgagcgc
      841 gggcgagccg gaaatagctc gttcagcaac gaccggttcg attcgttcga agatttctcg
      901 ctctacgatc gctgtatcgg ccgcggcatc agcaacggta tgaacgccgc gatttacggc
      961 aacggcattc aagtcttcca aagcccgggc gtggtgacga tcacgtacga gatgatccac
     1021 gagacgcgcg tgatcaagct cgacaactcg ccgtatcccg agatcgcgca gttcacgggc
     1081 aacgcgcgcg gccactggga gggcgacacg ctcgtcatcg agagtcgcgg cttccaggat
     1141 cgcacgagcg tcggcggcgc cccgaacagc gcgaacctgc gcacgacgga gcgcatacgc
     1201 cgcgtcgatc cggagatgct cgagtaccgc atcacgatca acgacccgga gacgtacacg
     1261 gcgccgttca cggtgcgcac gatgtggacg acgcagccgg actactacgc ttacgagtac
     1321 tcgtgccacg aaggtaactt cgcggtcggc ggcggtctcg ccggcgagcg cgcgctcgat
     1381 cgcgagaagg cggcagcgat cgcggcgggc aaaccgttgc ccgagcgttc gcgcgcgaac
     1441 atctacggcc gcccggaaga aggcgccgag atcttcgaca tcaacgcggg cgagtagcga
     1501 agaggcgcag cgcgagggcg cgtcgcgtcg cgagagccgc cgaaggctac tcgcgagaca
     1561 cgccggaagt gcgtccctgc ggctcgtgtg cgcacctccc tgtgcgcaca cggtctcgcg
     1621 agcagccttc ggcgtctctc gcgattcaac tctggtcgcg atcgtttaga tctgcatgcc
     1681 ccagcggcgc acggtgcggt gctcgatcgt gcggaagacg acgttctcga cgaagaggcc
     1741 gaagatgatg acggtcagta ggcccgcgaa gacttcgggg atccgaagct gattcttgtt
     1801 ctcgaagatg taccagccga gcccgcccga gccagagctc acgccgaaca cgagctccgc
     1861 cgcgatcagc gtgcgccacg cgaacgccca gccgactttg agccccgtca ggatgctcgg
     1921 gaacgcgccc ggtatcagga tgcgggcgat atagcccgcg ctcgagaggc cgtagttctg
     1981 cccgaccatg cgcagcgtcg acgacagcgc gcggaagccc gcgaacgtgt tcagcgctac
     2041 ggcccagagc accgcgtgca cgagcacgaa tatgacgctc gagtcgccga gcccgaacca
     2101 gatcagcgcg agcggcagca gcgcgatcga cggaaggggg ttgaacatcg ccgtcagcgt
     2161 ctcgagcagg tcggcgccga tgcgcgagac gctcgcgaac gccgtcagca cggcggcgaa
     2221 gaagagcccg gcgacgtagc ccttcagcaa caacgacagc gtgtagaggc ccgcggccgg
     2281 gatctcgccg cgagcgatgc cggaaaggag cgcgccgacg gtcgccgtga acggcgggaa
     2341 cagcagcgga ttgccgagcg cgcgcgcgta gatctcccac gcggccgcga gcagcgcgag
     2401 cagcacggat ttgcgcaccg cgccgttgcc gtagaggcgc gaccagaaac cgagcttcgc
     2461 tgccgcgacc gcgaacccgc tcgcgtcggc gtcgggtgtg tgcaagatct cgggcctctc
     2521 ggccgcgcgt tgctcagaca tcggcgatgg ctcccgtgcg gtcgaatagg aggcgctgaa
     2581 ttcgcgcgga cgtgagctcg ccgccggcgc ccgcgacgtc gacgccctcg cagttgatct
     2641 cggctttcgt gcgtcccgga tgcggcgtca ggagcagaat ccgattgccg atgcggatcg
     2701 cctccgggat cgagtgcgtg acgaacagaa ccgtgaaccg cgcctcgttc cagagctcga
     2761 gcaagtcttc ctgcatcttg ccgcgcgtca gcgcgtcgag cgccgcgaag ggctcgtcca
     2821 tgagcaggat ctgcggctcc atcgccatcg cgcgcgcgat cgcgacgcgc tgcttcatgc
     2881 cgcccgacag cgtgtgcgga aagctgtcgg caaatttcgt caggccgact ttggcgatga
     2941 agtgcagcgc gcgctcggtc gccgccttct tcgcgagctt gccgctcgcc gtcagcgcga
     3001 acacgacgtt gtcgcagacc gttttccacg gcaacagctg gtcgaattcc tggaagacga
     3061 acgcgcggtc gggcccggga cgtcccacgc gcgcgccatt caacacgatc tcgccctcga
     3121 cgggcgagag ataaccgccg atcgctttca gcaacgtcga cttgccgcag cccgaggggc
     3181 ccaagacgac gaagcgatcg cctttcagca cgtcgaagct cacgcgatac gtcgcggtca
     3241 cgagctgctg gcccgtgcgg tattgcaacg tcacgccttt gacggagaga agcgccggtt
     3301 ctcgcatcgc gcccggagta tacctggctg cacgtcccag ctcgttcgag cgctggcttc
     3361 cgcgccgcga atcgtggcag agtcgcggag cacgacaaga acaactacaa agagtggcac
     3421 gttgagcaca ccgca
//
LOCUS       DASSNN010000241         6056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_785418, whole genome shotgun sequence.
ACCESSION   DASSNN010000241 DASSNN010000000
VERSION     DASSNN010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785418"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VFL84_04365"
     CDS             complement(<1..904)
                     /locus_tag="VFL84_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607888.1"
                     /translation="MNMKIAVLLGILVLFHQAAWGATRADDGKLSSRSEALIEPFLGD
                     FLGSLNTAETPSPYDDLNHSLCGGPDVPPCRDEYGNPTPHLSPLANVIFRVSRNTDGK
                     IQAAIFQTTEQYRNNRPIDLIGQYCDSSIGDLAEYDFTSKDGQVAVAHLSFPIHPGKC
                     GDRLGYSLGSNFELTITRRPGEEKASLLEVRLYRGSENQDRTFVKRDTGETYEVRMKF
                     EPRKYDEFERDYFQRNYEFCPVDENGELIPDSKDCFTRAPERSGLVLPTTTGFPFFTW
                     DTVQDPKVRVKKRPRKKTYHEGTFY"
     gene            complement(941..3445)
                     /locus_tag="VFL84_04370"
     CDS             complement(941..3445)
                     /locus_tag="VFL84_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607889.1"
                     /translation="MALRWLDDLAQDLRKSARMLRTNRGFSATTIVTLALGIGASTLI
                     FSVVDAVLLRPLTYDDAEQIYRIRTLDAQGQPLGAVGRTLIDPIADSRQSADAAFYGF
                     SNESSVINREGTAFAISEYRTSEQFFRVFTNPFSLGRGFEPGDDIDKAVLSYQTWRDV
                     FGADPSIVGSPIQVNGADLMVIGVAGEGFEFPVGTAMWTKIFTGAGSQSLFNMEGYAR
                     VRPGVTGAQLRAELDAVAGELGIQRGDRPLQTAVRPLIEEIVGDFKGTLFMISGATAI
                     LLLIACLNVANLLLTRGMVRMGEIGLREALGARRVRIFRLLMTESVVLCALGGALGLA
                     LAMGAVRVLQAIGPGDLPRFDTVTIDRNTFLFATGCAVLTALLAGVAPAIRLSWRDLS
                     GSINAGVRAGSSGPGRNRIFGALVVVETSLAVMLVIGAGLLVRSYTALSSTDPGFDPD
                     RLLTLMLNVPGRVDIQDVRFDGQGRPTGYVGTGYLPVARFYQELAQRIAALPGVESVG
                     GTQFVPLNTGRFPSTPAPYRRVDSGRDPNEAPLLAYNNQVMPSFFATLGIRPVAGRVL
                     EPTDSRDAAGVVVVNEAFVRAYFDGADAVGRRIGLPNPGATRIGGVAFQLMERMNDEA
                     EIVGVIPDVKQAKLSDPVAPAVYIPHEQWTTRRLALLVRASIDDPASLIPQIRSELAA
                     MDSTIPPVFSVNSDVISSSLARHKLGAILLVVFGVVSLTLAAVGLYGLVSYSVRQRGN
                     EIAVRSALGADRDRVVKMFLGNGLLLALIGIVLGLAGAVALRQLVSSQLYGVSPLDPQ
                     VLAIVPIAMLCVALLASYVPARRASRVNPSTALREN"
     gene            3942..5498
                     /locus_tag="VFL84_04375"
     CDS             3942..5498
                     /locus_tag="VFL84_04375"
                     /inference="COORDINATES: protein motif:HMM:NF019436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607890.1"
                     /translation="MNTWTAPSLVGYAYDYKRNPGTQRVGDTHSGPNAGVYKVGASQW
                     LYGDDRKAWDESVNWFDYLVAQGFTVATWSQPQPMFADALPSAREAVETFLADTAARS
                     PEAPPPVVLLGHSRGGLLIRQVLKEQGSGGGRVQAVVTLNSPHHGSELGRAPGRMMAE
                     VVDLLDCCIPGDLTAPIKDELKDVVTELARPITKWLSDEEWVGDDESRELTPDGPLMR
                     GIAEGEQAVPGVRYYTYGGTNPTYYKMYVWVFDAMSAVPQYRGATQYFVWRAAPQEIG
                     PLSPIMDDLRDFADEVTPGRGDGLVSDVSARLPWAQHTTKSLNHAEVLWDRPLQQEVA
                     RIIASTGGDGSRRSSPAVTASSEATPVSPSAGRSPADSCCVVVVNPAMRGRLGRVVVA
                     FPDEVVAEVSGARIEVYRAGETQPAASGYGNQAFDLLPGTYEIAISGKRVTGVAVRSG
                     SDTQIGVGVLRVSASDGTRIEVVEPETGRSLVAGYGTMSFGLPSGEVGVRIAGQTETV
                     RIVAGQVAEF"
     gene            complement(5507..>6056)
                     /locus_tag="VFL84_04380"
     CDS             complement(5507..>6056)
                     /locus_tag="VFL84_04380"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7607891.1"
                     /translation="RGVREGSLNYERSQNVDTASKLAAELTKAGRHEEAIRVCTEART
                     GLFEDDPKILLALASAQSAAGRYTDAIATLDYLREKNPGFRSADGHLMYARALEESGA
                     TARAIEEYAALANYYPGAEARVRQAMLHKKLGEKARADALFAALLKDARLAPKHFRRA
                     EREWIELAERESREAPGQSSAS"
BASE COUNT         1062 a         1957 c         1994 g         1043 t
ORIGIN      
        1 tgtagaacgt gccttcgtga taggtctttt tgcgcgggcg ctttttgacg cggactttgg
       61 gatcttgaac cgtatcccac gtgaagaaag ggaaaccggt cgtcgtcggc aagaccagcc
      121 ccgaccgttc cggcgctcgt gtaaaacagt ctttggagtc cgggatcaat tcgccgtttt
      181 catcgaccgg acaaaactcg taattccgct gaaagtagtc gcgttcaaac tcgtcgtatt
      241 tgcgcggctc gaatttcatg cgaacttcgt acgtttcgcc ggtatcccgc ttgacgaagg
      301 tccgatcctg attttcgctt ccgcgataca gccgaacttc gagcagagaa gccttttcct
      361 cccccggcct tcgagtaatt gtcagctcga aattgcttcc gagtgaatat cccaatcggt
      421 cgccacactt tccggggtga atcggaaaac tgaggtgagc gacggcaact tggccgtctt
      481 tgctcgtgaa gtcatattcg gcaaggtctc cgatgctgct atcgcaatac tgcccgatga
      541 gatcgatcgg ccgattgttt cggtactgct ccgtcgtttg aaaaattgca gcttgaatct
      601 ttccgtcggt gttgcgggag actctaaaaa taacgttcgc aagcgggctc aagtgcggcg
      661 tcggattgcc gtattcgtcc cgacacggag gaacgtccgg tccgccgcac aggctgtggt
      721 tcaggtcgtc atagggggac ggcgtttccg ccgtattcaa cgagcctaga aaatctccca
      781 agaaaggttc gatgagagct tcgcttcgag aagacagctt tccatcgtcg gcccgagtcg
      841 cgccccaggc cgcctggtga aaaagaacca atattccaag caaaactgcg attttcatgt
      901 tcattgatct tatccgaatt ggccctcgaa aaagatcgac tcagttttcg cgcaacgcag
      961 tcgacggatt cacgcgagag gctctgcgcg ccggcacgta tgacgcgagc agcgcgacgc
     1021 acaacatcgc gatcggcacg atcgcaagca cctgcggatc gagcgggctg accccgtaca
     1081 gctgactcga gaccagctgc ctgagcgcca cagcgccggc gagcccaaga acgatgccga
     1141 tgagcgcgag cagcagcccg ttgccgagga acatctttac gacgcgatcg cggtcggcac
     1201 cgagcgcgga gcgcacggca atctcgttgc cgcgctggcg gacggagtac gacacgaggc
     1261 cgtagagccc cacggcggct agcgtcagcg acacgacgcc gaacacgacg agcaagatcg
     1321 cgccgagctt gtgccgcgcc agcgaggaag agatgacgtc cgaattcacg gagaacacgg
     1381 gcggaatcgt cgagtccatc gccgcgagct cgctccggat ctgcggaatc aagctcgcgg
     1441 gatcgtcgat cgacgcgcgc acgagcaacg caaggcgccg cgtcgtccac tgctcgtgcg
     1501 ggatgtacac cgcgggcgcc accggatccg agagcttggc ttgcttcacg tccggaatca
     1561 cgccgacgat ctccgcctcg tcgttcattc tttccatgag ctgaaacgcg accccgccga
     1621 ttctcgtggc gccggggttc ggcaagccga tgcgccggcc gacggcatcg gcgccgtcga
     1681 agtacgcgcg gacgaaagcc tcgttgacga ccacgacgcc ggccgcgtcg cggctgtcgg
     1741 tgggttcgag cactcggccc gcgaccgggc gtatccccaa cgttgcgaag aatgaaggca
     1801 tgacctgatt gttgtaagcg agcagcggcg cttcgtttgg gtcgcggccg ctgtcgacgc
     1861 gacggtaggg cgcgggggtg ctcgggaagc gtcccgtgtt gagcggcacg aattgcgtcc
     1921 cgccgaccga ctcgacgccg ggcaatgccg cgatgcgctg cgcgagctct tggtagaagc
     1981 gcgccacggg taggtacccg gtcccgacgt aacctgtcgg tctgccctgg ccgtcgaagc
     2041 gtacgtcctg aatgtcgact cgtcccggca cgttcagcat cagagtcagc aggcgatcgg
     2101 gatcgaagcc cgggtcggtg gacgagagag cggtgtagct gcgcacgagc agtcccgctc
     2161 cgatcacgag catcaccgcg agcgacgtct ccacgacgac gagcgcgccg aagatccggt
     2221 tgcgtcccgg tcccgacgag ccggcccgca cgcccgcgtt gatggagccg ctcaagtcgc
     2281 gccacgagag gcggatggcc ggcgcgacgc ccgcgaggag cgccgtcaga acggcgcagc
     2341 cggtcgcgaa caagaacgtg ttccgatcga tcgtcaccgt gtcgaagcgc ggaaggtcgc
     2401 cgggcccgat ggcttgcagc actcgaacgg cgcccatcgc gagcgcgagc ccgagcgcgc
     2461 cgcccaacgc gcacagcacg acgctctcgg tcatgagcaa acgaaagatc cggacgcgac
     2521 gagcgccgag cgcctcgcgt aggccgatct cgcccattcg caccatgccg cgagtgagca
     2581 ggaggttcgc gacgttcaag catgcgatca acagcaagat ggcggtagcg cccgagatca
     2641 tgaacagcgt gcccttgaag tcgccgacga tctcttcgat gagcggccgg accgcagtct
     2701 gtaacggccg gtcgccgcgc tggattccga gctcgccggc caccgcgtcg agctccgcgc
     2761 gcagctgcgc gccggtgacg cccgggcgca ctcgcgcgta gccttccatg ttgaacaggc
     2821 tctggctgcc cgctccggtg aaaatcttcg tccacatcgc ggtgccgacc ggaaactcga
     2881 agccctcgcc ggcgacgccg atgaccatga gatcggcgcc gttcacctga atcggcgaac
     2941 cgacaatgct cgggtccgcg ccgaagacgt ctcgccacgt ctggtagctc aacaccgcct
     3001 tgtcgatgtc gtcgccgggc tcgaagcccc ggccgaggct gaagggattc gtgaacacgc
     3061 ggaagaactg ctcggacgtt cggtactccg aaatcgcgaa cgccgtgccc tcgcggttga
     3121 tgacggacga ctcgttggaa aacccgtaga acgccgcgtc ggcggactgg cgcgagtccg
     3181 cgatggggtc gatcaacgtg cgcccgaccg ccccgagcgg ctgtccctgc gcatcgagcg
     3241 tgcgaatgcg gtagatctgc tctgcgtcgt cgtaggtgag cgggcggagg agcaccgcat
     3301 cgacgacgct gaagatcagc gtgctcgcgc cgatgccgag cgccagcgtc acgatcgtgg
     3361 tggcgctgaa accgcgattc gtgcgcagca tgcgcgccga cttgcggagg tcctgggcga
     3421 gatcgtctaa ccagcgcaac gccataggtt ggccctcccg acggcgccta cctcggcgag
     3481 cgacggctcg tcgaacgcga cgccttcccg cggtgactac tgtacgcgat gcgccggaac
     3541 ggcggccagc ttggtgctgc tgtaacgaac tggagcgccg cgcgactctt gggttagcca
     3601 ttccgcgaga gctctgttcg agctcgagtg gaagcacttt gcagccggag aagatgaaca
     3661 tgcgcggtcg aacggtgtac ctgcttccac tggctgtgct cgccatcgca ctcgcgcgcg
     3721 tcggtccagc gggcgctcag ctcgggggcg cgcgcgagcg tgcgccggcg tcgacgtcga
     3781 catccagcgt tcccggcaac cttgacgctg cgctcgccac gggcggatgc ccgccgagcg
     3841 tcttcttctg gtggaggcag catggcgaag gcggccggat cgtgtgcgcg agcgatccga
     3901 cgcgtcccgc ggtgctgctt tttcatgggc tccaccagga tgtgaatacc tggacagcgc
     3961 cgtcgctcgt cggctatgcg tacgattaca agcgcaatcc cggcacgcag cgcgtcggcg
     4021 acacgcattc aggcccgaac gcgggcgtgt acaaagtcgg cgcctcgcag tggttgtacg
     4081 gcgacgaccg caaagcctgg gacgaatccg tcaactggtt cgactacctc gtcgcgcagg
     4141 gctttacggt cgccacctgg agccagccgc agccgatgtt cgccgacgcg ctaccctcgg
     4201 cgcgcgaagc ggtcgaaact ttcctcgccg acactgcggc tcggagtccc gaggctccac
     4261 cccccgtggt cctgctcggc cacagccgtg gcggactcct gatccgtcag gtgctgaagg
     4321 agcagggcag tggcggcggc cgcgtgcaag ccgtcgtgac tctcaactct ccgcaccacg
     4381 gcagcgagct tgggcgcgcg ccgggacgaa tgatggccga ggtcgtggat cttctcgact
     4441 gctgcattcc aggcgatctc acggccccta tcaaggacga gctcaaggat gtcgtcaccg
     4501 agctcgcgcg gccgatcacg aagtggttgt cggacgagga gtgggtcggc gacgacgaaa
     4561 gccgggagct cacgcccgac gggccgctca tgcgcggcat cgccgaaggt gagcaggccg
     4621 ttcccggagt gcgctattac acgtacggcg gcacgaaccc gacttactac aaaatgtacg
     4681 tctgggtatt cgatgcgatg agcgcagtgc cgcaataccg cggtgctacg cagtatttcg
     4741 tctggcgcgc ggcgccgcag gagatcgggc cgctctcgcc gatcatggac gacctgcgcg
     4801 acttcgccga cgaggtgaca cccgggcgcg gagacggtct cgtcagcgac gtcagtgccc
     4861 gcctgccgtg ggcgcagcac acgacgaaga gcctcaacca tgccgaagtg ctctgggatc
     4921 gtccgctgca gcaggaagtg gcgcgcatca tcgcgagcac gggcggcgac ggatcgcggc
     4981 gctcgtcccc ggctgtcact gcttcgtccg aagcaacacc ggtcagtccg agcgccggcc
     5041 ggagccctgc agactcgtgc tgcgtcgtcg ttgtgaatcc ggcgatgcgc ggacgtctcg
     5101 gccgcgtcgt cgtcgccttt ccggatgaag tggtcgccga agtgtccggc gcgcgcatcg
     5161 aggtgtatcg agccggcgag acgcagccgg ccgcaagcgg gtacggcaac caggcattcg
     5221 atctcttgcc cgggacctat gagattgcga tttcggggaa gcgggtgacc ggcgtcgcgg
     5281 tgcggtcggg cagcgacacg cagatcgggg tcggcgtgct gcgggtcagc gcgagcgacg
     5341 gcacgcggat cgaggtggtc gaacctgaaa ccggacgatc gctcgtcgcc ggttacggca
     5401 cgatgtcctt cggcctgccg agcggcgagg tcggcgtgcg gattgcgggc cagaccgaga
     5461 cggtccgcat cgttgccggg caggtcgccg aattctgacg ccgcggtcag cttgcgctgc
     5521 tttggcccgg cgcttcgcgg ctctcgcgct cggcgagctc gatccactcg cgctcggcgc
     5581 gccggaaatg cttcggcgcg agccgcgcat ctttcaacag tgccgcgaac agcgcgtccg
     5641 cgcgggcttt ttcacctagc ttcttgtgca acatcgcttg gcgcacgcgc gcctcggcgc
     5701 ccgggtagta gttcgcgagc gccgcgtact cctcgatcgc gcgcgcagtc gcgccgctct
     5761 cctcgagcgc gcgcgcatac atgaggtgcc cgtccgcgga gcggaacccg ggatttttct
     5821 cgcgcaaata gtcgagcgtc gcgatcgcgt ccgtgtatcg accggccgcg gattgcgcgc
     5881 tggcgagcgc gagcaagatc ttcgggtcgt cctcgaacag gcccgtgcgc gcctcggtgc
     5941 agacgcggat tgcttcttcg tgccggccgg ccttcgtcag ctcggcggcg agcttgcttg
     6001 ccgtgtcgac attctggctg cgctcgtaat tcagcgagcc ttcgcgcacg ccgcga
//
LOCUS       DASSNN010000242         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1431521, whole genome shotgun sequence.
ACCESSION   DASSNN010000242 DASSNN010000000
VERSION     DASSNN010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431521"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..713)
                     /locus_tag="VFL84_04385"
     CDS             complement(93..713)
                     /locus_tag="VFL84_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7607892.1"
                     /translation="MQARIAAIILCSFAGAASAQVGTSMGLLDANSATEAELRAANVD
                     AAVVTKITASRPFASASALDELLAPTLSEQQRDELYGRVFVTINLNTASRAEILLIPG
                     LNERMAHEFEEYRPYTSLEQFRREIGKYVPANEVARLEQYVFVPMSLNKATGEAFMTI
                     PRMTQRMVHEFEEYRPYTSFDQFRREIGKYVDQKEVARYERYLTIE"
     gene            754..1362
                     /locus_tag="VFL84_04390"
     CDS             754..1362
                     /locus_tag="VFL84_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET7607893.1"
                     /translation="MRSRVARALDSYALLAALLALPWALLAYGYLTGRLLYGEVVHAS
                     GDWAIWCLMAALAVTPLHRLFPRRPWTAWLLPRRRYLGVATFAYAALHAAVYVLRQGE
                     LPRILAEALEAGLLTGWLAFAIFVPLALTSNDVSVRMLGPAWKRLHRAVYAAAVLSFA
                     HWILEAFDPTVAYAHVAVLAAIQSARLAPAARRLFNPTRFHS"
     gene            1410..>2383
                     /locus_tag="VFL84_04395"
     CDS             1410..>2383
                     /locus_tag="VFL84_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607894.1"
                     /translation="MSTRTNTILVAAAVVVCGAVVAQPASSKVDPQSQLVAQIDKLRT
                     ESGATTPELIAPLHVLGLLYQETDQHALAIVALEEARHVTRVQHGLSSVDEALLLKQQ
                     IRSEHALRNSDRAWDLEQDMLTIARKHIDDTRMVPIFRGLADDRSELLREFRAGKRPP
                     ELFIGCYFSGTHPRYDDPRDEQRPADADSCRFGTREAVILKLREEILMYYADAIEAIL
                     KNGDYGSAELRDLEKQALRVAFPAEYLVSPTTGNAMLGNTRFFGVDSRCSAATLDELL
                     ALPLLDSCLEAVVRKESHVLANVGSWVSLVRLIGYELRSGAPAADRGGA"
BASE COUNT          366 a          808 c          777 g          432 t
ORIGIN      
        1 gccggaacga tcgccgattc ggcccccgta tccgtgcgct accatttgcc gcagtgagcg
       61 ccgcgacctg ctagctcgaa cgccgtgggc ggctactcga tcgtgaggta gcgctcgtag
      121 cgcgcgactt ccttctgatc cacgtacttg ccgatctcgc ggcggaattg atcgaagctc
      181 gtgtacgggc ggtactcctc gaactcgtgc accatgcgct gcgtcatgcg cgggatcgtc
      241 atgaacgctt cgcccgttgc cttgttcagg ctcatcggca cgaacacgta ttgctcgagc
      301 cgcgcgacct catttgccgg cacgtacttg ccgatctcgc gccgaaactg ctcgaggctc
      361 gtgtacggcc gatactcctc gaactcgtgt gccattcttt cgttcaagcc gggaatcagc
      421 aggatctcgg cgcgcgaggc cgtgttcaag ttgatcgtca cgaacacgcg gccgtacagc
      481 tcgtcgcgct gctgctcgct caacgtcggc gcgagcagct cgtcgagagc gctcgcgctc
      541 gcgaaaggcc ggctcgcggt tatcttcgtg acgacggccg cgtcgacgtt cgcggcgcgc
      601 agctcggctt cggtggcaga attggcgtcg agcagaccca tgctcgttcc gacttgcgcg
      661 gatgccgcgc cggcgaacga gcacagaatg atggcggcga tgcgggcttg catggagagt
      721 cccctttcca agttgtcgat tcgcggagcg tgaatgcggt cgagagtagc gagagcgctc
      781 gattcctatg ccttgctcgc ggcgctgctc gcgctcccgt gggccttact ggcatacggc
      841 tatcttaccg ggcgtttgtt atatggggag gtggtccacg cgtcgggcga ttgggcaatc
      901 tggtgcctga tggccgcgct ggccgtgacg ccgctgcacc gccttttccc gcgccggccc
      961 tggacggctt ggctcctgcc gcggcgccgc tacttgggcg tcgcgacctt cgcctacgcg
     1021 gcgctgcacg cggcggtcta cgtgctgcgc caaggcgagc tgccgcgcat cctcgccgaa
     1081 gcgctcgagg ccggcttgct gacggggtgg ctcgcgtttg cgatcttcgt gccgctggct
     1141 ttgacgagca acgacgtctc cgtacgcatg ctcgggccgg cgtggaagcg gctgcatcgc
     1201 gccgtctacg ccgccgccgt cttgagcttc gcgcactgga ttctcgaagc gttcgacccg
     1261 acggtcgcgt acgcgcatgt cgccgtgctc gccgcgattc agagcgcgcg cctggcgccg
     1321 gccgctcgcc gactgttcaa tccgacgcgc ttccacagct agcggcggct tgcgccgtgc
     1381 gcggggaggt gccagccttc cgcaagtcga tgagcacgcg cacgaacacg attctcgtcg
     1441 ccgccgccgt cgtcgtatgc ggcgccgtcg ttgcgcagcc agcgtcgagc aaagtcgatc
     1501 cgcagtcgca gctcgtcgcg cagatcgaca aattgcgtac ggagagcggt gctacgacgc
     1561 ccgagctgat cgcgccgctg cacgtgctcg gcctcttgta ccaagagacc gatcagcacg
     1621 cgctcgcgat cgtcgcgctc gaggaggcgc ggcacgtgac gcgcgtgcag cacggcttgt
     1681 cctccgtcga cgaagcgctg ctgctcaagc agcagattcg cagcgagcac gcactgcgca
     1741 acagcgaccg ggcgtgggat ctcgagcagg acatgctcac gatcgcgcga aaacacatcg
     1801 acgacacgcg catggtgccg atcttccgcg ggctcgccga cgatcggtcc gagctcttgc
     1861 gcgagtttcg cgccggcaag cgcccgccgg agctctttat cggctgctac ttctccggga
     1921 cgcacccacg ctacgacgat ccgcgcgacg agcaacggcc cgcggacgcg gacagctgcc
     1981 gattcggaac gcgcgaagcg gtcatcttga agctgcgcga agagattctg atgtactacg
     2041 ccgatgcgat cgaggcgatc ctcaagaacg gcgactacgg cagcgccgag ctccgcgatc
     2101 tcgagaagca agcgcttcgc gtcgcgttcc cggccgagta tctcgtgtca ccgaccacgg
     2161 gcaacgcgat gctcggcaac acgcggttct tcggagtaga cagccgctgc tcggccgcga
     2221 cactcgacga gcttctcgcg ctaccgctcc tcgacagctg cctcgaagcc gtcgtccgca
     2281 aagagagcca cgtgctcgcg aacgtcggca gctgggtcag cctcgtgcgt ttgatcgggt
     2341 acgagctccg cagcggcgcg ccggcggccg accgcggcgg cgc
//
LOCUS       DASSNN010000243         3243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_548551, whole genome shotgun sequence.
ACCESSION   DASSNN010000243 DASSNN010000000
VERSION     DASSNN010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548551"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /gene="obgE"
                     /locus_tag="VFL84_04400"
     CDS             <1..989
                     /gene="obgE"
                     /locus_tag="VFL84_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET7607895.1"
                     /translation="FRREKFVPRGGPDGGDGGDGGSIYLVAREGVNTLADFRVKRRFR
                     AENGRGGSGKDMAGAAGADLYIEVAKGTEVYDVETEEKLGDLVADGDTLLVAKGGQGG
                     RGNTRFKSSVNRAPRQFDVGGEGERRKLRLALKLLADVGLLGAPNAGKSTLTRAISAA
                     RPKVADYPFTTLHPQLGVVRVDADRSFVVADIPGLIEGAAAGAGLGIQFLKHLERTRL
                     LLHIVDACAELSGGDVVETYRAVEGELGQYSDALADRPRWLVLNKLDLLPPAERKKRT
                     AAIVKKLKWKGPVYGISALTADGTKRLVQDAMSYLEGQRERDETAKPSGHGA"
     gene            complement(972..1235)
                     /gene="rpsT"
                     /locus_tag="VFL84_04405"
     CDS             complement(972..1235)
                     /gene="rpsT"
                     /locus_tag="VFL84_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006993166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET7607896.1"
                     /translation="MANIQSAKKRARQAIKRRAHNVALRSQVRTAVRKVMKTIESGNK
                     EAAKAEFAAVVPQIDRMATKGILRKSRAAHYKSQLNARLRAMA"
     gene            1330..2928
                     /gene="murJ"
                     /locus_tag="VFL84_04410"
     CDS             1330..2928
                     /gene="murJ"
                     /locus_tag="VFL84_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET7607897.1"
                     /translation="MTATSNAGSARPQERLLTATTVVGFMTVLSRITGLARDVAFSAW
                     FGATPIMEAFIVAFRVPNLLRRFFAEGAFQQAFVPVVSEYRAHRTHAETRELVSYGAG
                     TLGVVLFAVSAVGVVAAPLLILAFAPGFVSKQGQFDLAVEMLRFTFPYVLFVSLTAFA
                     GSVLNAHRRFAVAAFTPVLLNVVMIVFAGLVGPLLENPALGLAGGVFAAGVVQLAFQL
                     PFLKRLDLLPRPRISFRHEGMRRILGLMLPSLFGSSVMQISILLDTLIASFLLAGSVS
                     WLYYSDRLIEFPLGVLGIALATVILPRLSEHHATQSAQTFSDTLDWALKLVVVIGVPA
                     SIGLALLAEPLMATLFLRGEFTPRDVEMAAASLRAYAPGLVAFILVKVLAPGYFARQD
                     TRTPVRVGVQSLVLGMVLAVAFVLVLLRTEWAPAHAGIAAATACSALANAGLLLVGLR
                     KQGIYRAGADWVALAWRVAVPSLVMALALRFGRGFAGDWLDMGTIQRVASLAVLVGGG
                     AAVYFAACYLAGLRVSDLRMKPGA"
     gene            2936..>3243
                     /locus_tag="VFL84_04415"
     CDS             2936..>3243
                     /locus_tag="VFL84_04415"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HET7607898.1"
                     /translation="MRLYRTFASLRRSPSTKPRAVAIGVFDGLHIGHQAILERARGAA
                     HELAGSSLVLTFEPTPKEFFSPATAPPRLTRFRERFELLQALGIDELFCAKFGAVRDL
                     T"
BASE COUNT          452 a         1089 c         1122 g          580 t
ORIGIN      
        1 gctttcggcg cgagaaattc gtgccgcgag gcggccccga cggtggcgat ggcggcgacg
       61 gcggcagcat ctatctcgtc gcgcgcgagg gcgtcaacac gttggccgac ttccgcgtga
      121 agcggcgctt ccgcgccgag aacggccgcg gcggctccgg caaggacatg gcgggcgcgg
      181 ccggcgccga tctctacatc gaggtggcca aaggcaccga agtctacgac gtcgagaccg
      241 aagaaaagct aggcgatctc gtcgcggacg gcgacacgct gctcgtcgcg aaaggcgggc
      301 agggcggccg cggcaacacg cgtttcaaga gcagcgtgaa ccgcgcgccg cgccagttcg
      361 acgtcggcgg cgaaggcgag cgccgcaagc tcaggctcgc gctgaagctc ctcgccgacg
      421 tcgggctgct cggcgcgccg aacgccggca agtcgacgct gacgcgcgcg atatcggccg
      481 cgcgcccgaa ggtggccgat tacccgttca cgacgctgca tccgcagctc ggcgtcgtgc
      541 gcgtcgatgc ggaccgcagc ttcgtggtcg ctgacatccc gggcctcatc gaaggcgcgg
      601 cggccggggc cggcttaggc attcagttcc tgaagcacct cgagcgcacg cggctgctgc
      661 tgcatatcgt cgacgcatgc gccgagctct cgggcggcga cgtcgtcgag acttatcgcg
      721 ccgtcgaggg cgagctcggc cagtattcgg acgcgctggc ggaccggccg cgctggctcg
      781 tgctgaacaa gctcgatttg ctgccgccgg cggagcgaaa gaagagaacg gccgcgatcg
      841 taaagaagct gaagtggaaa gggccggtct acggcatcag cgcattgacg gcagacggca
      901 cgaagcgcct cgtgcaggac gcgatgtcgt acttggaagg gcagcgcgag cgcgacgaga
      961 cggcgaagcc gtcaggccat ggcgcgtagc cgggcgttga gctggctctt gtaatgcgcg
     1021 gcgcggctct tgcgcaggat gcccttggtc gccatgcggt cgatctgcgg cacgacggcc
     1081 gcgaactcgg ccttcgcagc ttctttattg cccgactcga tcgtcttcat gaccttgcgg
     1141 acggccgtgc gaacctgcga ccgcaacgcg acgttgtggg cccggcgctt gatggcctga
     1201 cgagcgcgct tctttgccga ttgaatgttg gccaagcgag agttccgttg ggctggcgcc
     1261 ccgaaaaagg cggctattat tcggacctcc catagggcaa gtcaagcaaa gaacctcccc
     1321 ggaccgctca tgacggcgac cagcaacgcg ggtagcgccc gcccccagga acgtctgctc
     1381 acggccacga ccgtcgtagg cttcatgacc gtgctgtcgc gcatcacggg actcgcgcgc
     1441 gacgtcgcgt tctcggcgtg gttcggcgcg accccgatca tggaggcgtt catcgtcgca
     1501 ttccgggtcc cgaacctgct gcgccgcttc ttcgccgaag gcgcgttcca gcaggccttc
     1561 gtgcccgtcg tctcggaata ccgcgcgcac aggacgcacg cggagacgcg cgagctcgtg
     1621 agctacggcg cggggacgct cggcgtcgtg ctgttcgccg tgagcgccgt cggcgtcgtg
     1681 gccgcgccgc tcttgatcct cgcgttcgcg cccggcttcg tgagcaagca agggcagttc
     1741 gacctcgcgg tcgagatgct gcggttcacg tttccgtacg ttctcttcgt gtcgctgacg
     1801 gcgttcgccg gcagcgtgct gaacgcgcat cgccggttcg ccgtcgcggc gttcacgccc
     1861 gtgctgctga acgtcgtcat gatcgtattc gcgggtctcg tcgggcccct gctcgagaac
     1921 ccggcgctcg gcctcgcggg cggcgtgttc gcggccggcg tcgtgcagct cgcgtttcag
     1981 ctgccgtttt tgaaacgcct cgatctgttg ccgcggccta ggatctcgtt tcgccacgaa
     2041 ggcatgcggc gcatcctcgg cctgatgctg ccgtcgctgt tcgggtcgtc ggtcatgcag
     2101 atcagcatct tgctcgacac gctgatcgcg tcgttcctgc tcgcgggcag cgtgagctgg
     2161 ctctactact ccgaccggct gatcgagttt ccgctcggcg tgctcggcat cgcgctcgca
     2221 acggtcatac tgccgcgttt gtccgagcac catgcgacgc aatcggcgca gacgttctcg
     2281 gacacgctcg actgggcgct caagctcgtc gtcgtgatcg gcgtgcccgc gtcgatcggg
     2341 ctcgcgttgc tcgccgagcc gttgatggcg acgctgttcc tgcgcggcga gttcacgccg
     2401 cgcgacgtcg agatggcggc cgcgagcctg cgcgcctacg cgccaggcct cgtcgcgttc
     2461 atcctcgtca aagtgctcgc gccaggctac ttcgcgcgcc aggacacgcg cacgcccgtg
     2521 cgcgtgggcg tccaatcgct cgttctcggc atggtgctcg cggtcgcgtt cgtgctcgtg
     2581 ctgttgcgga ccgaatgggc tcccgcgcac gcgggaatcg ccgccgcgac tgcgtgctcg
     2641 gcgctcgcga acgcgggttt gctgctcgtt gggctacgca agcagggcat ataccgagct
     2701 ggcgcggatt gggttgcgct cgcgtggcgc gtcgccgtgc cgagcctcgt gatggcgctc
     2761 gcgcttcgct ttgggcgcgg tttcgccggc gactggctcg acatgggcac gatccagcgt
     2821 gtcgcgagcc tcgccgtgct cgtcggcgga ggcgcagccg tatacttcgc cgcgtgctat
     2881 ctcgccggct tgcgcgtgag cgatctccgc atgaagccgg gggcgtaaga gaggaatgcg
     2941 gctctatcgg accttcgctt cgctgcggcg gtcgccttcg accaagccac gtgcggtcgc
     3001 aatcggcgta ttcgacggcc tgcacatcgg gcatcaggca atcctcgagc gcgcccgcgg
     3061 cgcggcgcac gagctcgcgg gctcctcgct cgtgctgacg ttcgagccga cgccgaagga
     3121 gtttttctcg ccggcgaccg ctccgccgcg gctcacgcgc tttcgcgagc gtttcgagct
     3181 cctgcaggcg ctcggaatcg acgagctctt ctgcgcgaag ttcggcgccg tgcgcgacct
     3241 gac
//
LOCUS       DASSNN010000244         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1287522, whole genome shotgun sequence.
ACCESSION   DASSNN010000244 DASSNN010000000
VERSION     DASSNN010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287522"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /gene="yjgA"
                     /locus_tag="VFL84_04420"
     CDS             complement(<1..283)
                     /gene="yjgA"
                     /locus_tag="VFL84_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HET7607899.1"
                     /translation="MARKPRYEEPADEAEPDELEEQEGRGRSARKRASHELTRLGEEL
                     LRLRPDQFAALALPERLEEAVAEARRLTSFGAKRRQMLFIGKLMRQLDEA"
     gene            complement(297..1592)
                     /locus_tag="VFL84_04425"
     CDS             complement(297..1592)
                     /locus_tag="VFL84_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7607900.1"
                     /translation="MAQSATHAAIEAVFRIEWPKLIAGLTRVVRDVGAAEELAHDALV
                     AALEQWPEEGIPDKPGAWLMATAKHRAIDAVRRAKLVRRKHEELGGELEALGGEASDE
                     RDAIDEALDDQIGDDMLSLMFIACHPVLATAARVALTLRMVGGLTTAEIARAFLVPEP
                     TIAQRIVRAKRTLSEARVPFEVPRGEELAARLASVLEVVYLVFNEGYSATAGDDWLRP
                     ALCDDALKLARMLAELAPNEPEVHGLVALLEIQASRSAARTRADGEPVLLLEQDRSRW
                     DRLLIRRGLQALERAERLGGALGPYALQAAIAACHARAATAEATDWPRIVALYDALAA
                     LAPSPIVQLNRAVAVSMAFGAADAIAIVDALVESGELESYHLLCAVRGDLLVKLGRLR
                     EARAEFERAASLTRNERERRLMQARAEACGALVAPAPLA"
     gene            complement(1662..2075)
                     /locus_tag="VFL84_04430"
     CDS             complement(1662..2075)
                     /locus_tag="VFL84_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7607901.1"
                     /translation="MRFMILVKATRDSEAGTQPSETLFAEMAAYHEALAKAGVLLDGS
                     GLKPSSKGWRIRYSGDKRTIVDGPFAEAKELVAGYTMIETKTREEALEWARRYPNPSI
                     DRGEAEIEVRELFELEDFAPNPQLKRFEAIKELNP"
     gene            complement(2205..2504)
                     /locus_tag="VFL84_04435"
     CDS             complement(2205..2504)
                     /locus_tag="VFL84_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7607902.1"
                     /translation="MAKPKHFNDLAKRYPQYLAAVDQLGESVKHAGPLEPKVAELIQL
                     AAAAAVHSEGSVHSHARRARKAGATDDEINHAVLLLTSTIGFPSVAAALSWVDDT"
     gene            complement(2524..>2777)
                     /locus_tag="VFL84_04440"
     CDS             complement(2524..>2777)
                     /locus_tag="VFL84_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET7607903.1"
                     /translation="FVIPDILCNAGGVTVSYLEWVQNRIGYYWPEKRVNDDLQRIMEA
                     AFDDVYTTAQRSRCSMRIAAFMVAIRRVTEASEMRGLYA"
BASE COUNT          404 a          982 c          942 g          449 t
ORIGIN      
        1 ccgcctcatc gagctggcgc atcagcttgc cgatgaacag catttggcgc cgcttcgcgc
       61 cgaagctcgt gagccgccgc gcctcggcaa ctgcctcttc gagccgctca ggcaaggcga
      121 gcgcggcgaa ttggtccggc cgcaacctga gcagctcctc gccgagcctc gtgagctcgt
      181 ggctcgcgcg cttgcgagcg ctacggccgc gcccctcttg ctcttcgagc tcgtcgggct
      241 ccgcctcgtc tgcgggttct tcgtaccgtg gtttgcgcgc cattgagcga cgagtatcac
      301 gcgagcggcg ccggcgcaac cagggcgccg caggcctcgg ctcgcgcttg catgagccgg
      361 cgctcgcgct cgttacgcgt cagcgacgcg gcgcgctcga actcggcgcg cgcctccctc
      421 aggcggccga gcttcacgag caagtcgccg cgcacggcgc agagcaagtg ataagactcg
      481 agctcgccgc tctcgacgag cgcgtcgacg atcgcgatcg cgtcggcggc gccgaaagcc
      541 atcgagacgg cgacggcgcg attgagctgc acgatgggcg acggcgcgag cgccgcgagc
      601 gcgtcgtaga gcgcgacgat gcgcggccag tcggtggctt cggccgtcgc ggcgcgcgcg
      661 tggcatgccg cgatcgcggc ttgcagcgcg tacggcccga gagcgcctcc gagccgttcc
      721 gcgcgctcga gcgcctgcag gccgcgccga atcagcaagc gatcccagcg gctccggtct
      781 tgctcgagca gcagcacggg ctcgccgtcc gctcgggtcc gcgcggcgct gcgcgaagcc
      841 tggatctcga gcagcgcgac gagcccgtgg acctcgggct cgttcggcgc gagctcggcg
      901 agcatgcgcg cgagcttgag cgcgtcgtcg cagagcgcgg gccgcagcca atcgtcgccg
      961 gccgtggccg aataaccttc gttgaagacg agatagacga cctcgagcac ggaggcgagg
     1021 cgcgcagcga gctcctcgcc gcgcggcacc tcgaacggca cgcgcgcctc ggacagcgtg
     1081 cgcttcgcgc gcacgatgcg ctgcgcgatc gtcggctccg gcacgaggaa cgcgcgcgcg
     1141 atctcggccg tcgtgaggcc gccgaccatg cgcagcgtca gcgccacgcg cgcggccgtc
     1201 gcgagcaccg ggtgacaggc gatgaacatc aggctcagca tgtcgtcgcc gatctgatcg
     1261 tccagcgctt cgtcgatggc gtcgcgctcg tcgctcgcct cgccgcccag cgcctcgagc
     1321 tcgccgccga gctcctcgtg cttgcgccgc acgagcttcg cacggcgcac cgcgtcgatc
     1381 gcgcgatgct tcgccgtcgc catgagccac gcgccgggct tgtccggaat gccttcctcg
     1441 ggccactgct cgagcgcggc gacgagcgcg tcgtgcgcga gctcctcggc ggcgccgacg
     1501 tcgcgcacga cgcgcgtgag acccgcgatg agcttcggcc attcgatgcg gaacaccgcc
     1561 tcgatcgcgg catgcgtcgc cgactgcgcc atgccgtcaa gctcggagtg gaacgagctc
     1621 gcgcgcgagc gcgctcaatg cgcggccggc gatgacgcgc ctcacggatt gagctccttg
     1681 atcgcctcga agcgcttcag ctgcggatta ggcgcgaagt cctctagctc gaagagctcc
     1741 cgcacctcga tctcggcttc gccgcgatcg atcgaaggat tcggatagcg ccgtgcccac
     1801 tcgagcgcct cttcacgcgt cttcgtctca atcatcgtgt agcccgcgac gagctccttc
     1861 gcttcggcga acggcccgtc gacgatcgtg cgcttgtcgc ccgagtagcg aatgcgccag
     1921 cccttcgagc tcggcttgag gcccgagccg tcgagcagca cgccggcctt ggcgagcgcc
     1981 tcgtggtagg ccgccatctc ggcgaacagg gtctcgctgg gctgcgtgcc cgcctcggag
     2041 tcgcgcgtgg cctttaccaa gatcatgaaa cgcatgcgtc atctccttga tataaggagc
     2101 cggttggccg atctggccgt atctcgttcg gctctgacta gacgtcgaac gagcgagccc
     2161 gaaatcgaca agcgccgcgc gggcttgagc cacccggggc ggcgctaggt gtcgtcgacc
     2221 caggaaagcg ccgccgcgac gctcgggaag ccaatcgtgc tcgtcaggag tagcacggcg
     2281 tgattgatct cgtcgtcggt cgccccggcc ttgcgtgccc ggcgggcgtg gctatgcacg
     2341 gagccctccg agtgcacggc cgcggccgcc gcgagctgga tcagctcggc gaccttcggc
     2401 tcgagcggcc cggcgtgctt gacgctttcc ccgagctgat cgacggccgc caagtactgt
     2461 ggatagcgct tcgcgagatc gttgaagtgt ttcggttttg ccatcgttca ctctcctcgc
     2521 agctcaggcg tagagaccgc gcatctccga agcttccgtg acgcggcgaa tcgcgaccat
     2581 gaacgccgcg atacgcatgc tgcagcggct gcgctgcgct gtcgtgtaca cgtcgtcgaa
     2641 ggccgcctcc atgatgcgct gcaaatcgtc gttgacgcgc ttctcgggcc agtagtagcc
     2701 gatgcggttc tggacccact cgagatacga caccgtgacg ccgcccgcgt tgcagaggat
     2761 gtcgggaatg acgaatg
//
LOCUS       DASSNN010000245         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_45708, whole genome shotgun sequence.
ACCESSION   DASSNN010000245 DASSNN010000000
VERSION     DASSNN010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45708"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="VFL84_04445"
     CDS             <1..1262
                     /locus_tag="VFL84_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7607904.1"
                     /translation="EAAKAANAEIAALLLNAGADVNAANADGETALMLSARTGSVDVA
                     KQLIDAGADVNAREAWRDQTALMWAAEGAFVGVVDLLIAHGADVHARAAANDWGSQIT
                     SEPRAQYRPTGGLTPLLYAARSGCVECVRSILKAGESVDRPTPDGVTPLMLAIDNYEL
                     DTAKALLDAGANPHLSDWWGRTALYLAVDMNTYRPRGSEPHARSKSTTGMDLINALLS
                     AGVEVNPQLNFHRPGRGGNSARFVDDLLTTGATPLLRAAIMHDDDAMRALLAAGARVD
                     LPNVMGVTPLMAAAGVGVREPGFGANRAPDFTSKEIESEVIASLDILLDAGADVNAVI
                     SDTQSRTARIARQNSLTDRLGQTALHQVAGRGWADVVAHLLQRGANPAAKDALGRTPL
                     DLATTPVQGRPVPNGERIAEMLKAASR"
     gene            1426..2007
                     /locus_tag="VFL84_04450"
     CDS             1426..2007
                     /locus_tag="VFL84_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7607905.1"
                     /translation="MSAALLVRMLSAAALIGGLAFQPVASAVPIGDFSWGEHTEDECA
                     TGLCGSFFSVVNFSDVSFPPLGGTFGDVFVDLETTVGSSSLGLGEIVVGGSSQSIEDL
                     LDLSILSAALSLTFSAAPGSFQLLDLDGNAVAGLTAPGSLLIDYTVDASVPEPATLLL
                     FTAGLLGLGLGRRARQHTPATSRMKRTRSALVT"
     gene            2136..>2604
                     /locus_tag="VFL84_04455"
     CDS             2136..>2604
                     /locus_tag="VFL84_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607906.1"
                     /translation="MGASRSVAVAITGTGFDVIAGNNVVTFTPVTGAAVNATASAITT
                     LSAATGLRRLTVTVPAGLPVGTAALSVRNAVTGETSAGKSLEIVEILLTGVTSAAVGA
                     TNVNVQITGSPNSAFVAGSTRATFGAGVTVNSTTVESPTRLTANVSVSPTAAVG"
BASE COUNT          395 a          914 c          872 g          423 t
ORIGIN      
        1 gcgaggccgc gaaggccgcg aacgccgaga tcgccgcgct gctcttgaat gccggcgccg
       61 acgtcaatgc cgcgaacgcc gacggcgaga cggcgctgat gctgtccgcg cgaacgggct
      121 cggtggatgt cgcgaagcag ctcatcgacg ccggcgccga cgtcaacgcg cgcgaggcgt
      181 ggcgcgatca gacggcgctg atgtgggccg ccgaaggcgc gttcgtaggc gtagtggact
      241 tgctgatcgc gcacggcgcc gacgtgcacg cgcgcgccgc cgcgaacgac tggggctcgc
      301 agatcacgag cgagccgcgc gcgcaatacc gcccgaccgg tggcctcacg ccgctgctct
      361 atgccgcgcg ctcgggctgc gtcgagtgcg ttcgctcgat tttgaaagcc ggcgagagcg
      421 tcgaccggcc gacgcccgac ggcgtgacgc cgttgatgct cgcgatcgac aactacgagc
      481 tcgacacggc gaaagcgctg ctcgacgccg gcgcgaaccc gcacctgtcg gattggtggg
      541 gccgcacggc gctgtatctc gccgtcgaca tgaacacgta tcggccgcgc ggctccgagc
      601 cgcacgcgcg ctcgaagagc acgaccggca tggaccttat caacgcgctg ctctcggccg
      661 gcgtcgaggt gaacccgcag ctgaactttc accgccccgg ccgcggtggc aacagcgcgc
      721 ggttcgtcga cgacttgctg acgacgggcg cgacgccgct gctgcgcgcc gcgatcatgc
      781 acgacgacga cgcgatgcgc gcgctgctcg ctgcaggagc gcgcgtcgat ctgccgaacg
      841 tcatgggcgt gacgccgctg atggccgcgg ccggcgtcgg cgtgcgcgag cccggcttcg
      901 gcgcgaaccg cgcgcccgac ttcacgagca aggagatcga gagcgaggtc atcgcttcgc
      961 tcgacatcct gctcgacgcc ggcgccgacg tgaacgcggt catctccgac acgcagagcc
     1021 gcacggcgcg catcgcgcgg caaaactcgc tgaccgatcg tctcggccag acggcgctgc
     1081 atcaagtggc cggccgcggc tgggccgacg tcgtcgcgca cctcttgcag cgcggcgcga
     1141 acccggccgc gaaggacgcg ctcggccgca cgccgctcga tctcgcgacg acgcccgtgc
     1201 aaggccgccc cgtcccgaac ggcgagcgca tcgccgagat gctgaaggcc gcgtcgcgct
     1261 gattcgctcg cgcgcgtcga gaatacttac aacccgctga acgcttcggc gacggctcgc
     1321 tcgcagacgt ctgatttggc tcaaagtcct tccacgagca cgttccttgc atggttatga
     1381 cattcgcccc ggtgctccaa ctccaagaac gcaaggtgcc agcgaatgtc tgccgccctg
     1441 ttggttcgga tgctctccgc cgccgcgctg atcggcggct tggcgtttca acccgtggcg
     1501 agcgccgtgc cgattggcga cttcagctgg ggcgagcaca ccgaagatga gtgcgcgacc
     1561 ggcttgtgcg ggtcgttctt cagcgtcgtg aatttttccg acgtgtcgtt cccgccgctc
     1621 ggcggtacgt tcggcgacgt cttcgtagac ctcgaaacga ccgtcggctc gtccagccta
     1681 ggcttgggcg agatcgtcgt cggcggctcg agccagtcca tcgaagattt gctcgatctc
     1741 tcgatcctgt cggccgcgct gagcctgaca ttctcggcag cgccgggctc ttttcagtta
     1801 ctcgatctcg acggcaatgc cgtcgccggg ctgaccgcgc ccggctcgtt gctgatcgac
     1861 tatacggtcg acgcgtctgt tcccgagccg gccacgctgc tgctgttcac ggccggtttg
     1921 cttggactcg gcctcggtcg cagagccagg cagcacaccc ccgcaacttc aaggatgaaa
     1981 cgcacccgct ccgccctcgt aacctagcgg cgagcggcgt gaaagagctg gtccgaacgt
     2041 cggccttcct cttccggaca cgcgtcttct ttgccttcct gctgttcgcc gccgcgcacg
     2101 cggcacacgc cggcactctg ttgggagtga ctccgatggg tgcgtcgcgc tccgtcgcgg
     2161 tcgcgatcac gggaaccggt ttcgacgtta tcgccggcaa taacgtagtc acgttcacgc
     2221 ccgtgacggg cgccgccgtg aacgcgacgg cttcggcgat cacgacgttg agcgcggcga
     2281 ccggcctacg ccgcctcacc gtcacggtcc cggccgggct gccggtcgga acggccgcgc
     2341 tcagcgtgcg caatgcggtg acgggagaga cgagtgccgg caagtcgctc gagatcgtcg
     2401 agattctcct gacgggagtc acctccgccg ccgtcggcgc gaccaacgtg aacgtgcaga
     2461 tcacgggctc gccgaactcg gctttcgtgg cgggcagcac gcgcgcgacg ttcggcgccg
     2521 gcgtcaccgt caacagcacg acggtagagt ctccgacgcg attgacggcc aacgtctcgg
     2581 tttcgccgac ggcggccgtc ggtc
//
LOCUS       DASSNN010000246         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_786337, whole genome shotgun sequence.
ACCESSION   DASSNN010000246 DASSNN010000000
VERSION     DASSNN010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786337"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFL84_04460"
     CDS             <1..441
                     /locus_tag="VFL84_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607907.1"
                     /translation="LVFFVGLLVSVGAPVVRVRSGFYPLEVGGIVTLYNLTDMLLFGA
                     FLALAFAQRARPELHKRWIVAASAALLGAALGRVVPGNSPQYLLLWLSPLLALVALDL
                     ATRRRVHFIPVLGIALLVVAFFKVPLYSAAIWREVGSALLRPFV"
     regulatory      485..594
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            642..>2101
                     /locus_tag="VFL84_04465"
     CDS             642..>2101
                     /locus_tag="VFL84_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7607908.1"
                     /translation="MSFRFTRRTALALVAAAAAHAALGQSERVEEIVVTADFRESRIE
                     ELPVSVTVLDREELKAAAQQHFEEVIRSVPNLNLSGEGSRARYFQLRGVGELEQYEGA
                     PNPSIGFIVDDIDFSSLGSAATLFDVDRVEVLRGPQGTRYGANALGGLVYMRSQAPTP
                     TLSADLEATAGSDGTDALGAAFGGPIGDALGFRTSLQTYRNNGFRDNVYLGRDDTYGR
                     DELTARGKLAWSPSDDVQVDFTGLYVDVDNGYDAWAIDNGFNTYSDDPGRDAQQSTAG
                     SVRVQAALARVDLVSITGFADTDAVFSFDADWGNAGFWAPYVYDYFTANDRERRTASQ
                     ELRVLSKPGAIASGRGEWLAGVYVLDLEEGNDQTTTGVYEDPFCGPACSLLTDTAVRS
                     DYDATNLALFGQVAFDLNDRLGLTAGLRWERRSADSSDTSSNAFSPDDDMLGGELAFD
                     WQLAERRSAYVRLARGYKAGGFNVSLAGVDFGSVDN"
BASE COUNT          303 a          710 c          710 g          378 t
ORIGIN      
        1 ctcgttttct ttgtcggcct gctcgtcagc gtcggcgcgc cggtcgtgcg cgtgcgcagc
       61 gggttctatc cgctcgaagt cggcggcatc gtgacgttgt acaacctgac cgacatgttg
      121 ctgttcggcg cgttcctcgc gctcgcgttc gcgcagcgcg cacgccccga gctgcacaag
      181 cgctggatcg ttgccgcgag cgctgcgctc ttgggcgccg cgctcggccg cgtcgtgccg
      241 ggcaactcgc cgcagtatct tctgctgtgg ctgtcgccgc tgctcgcgct cgtcgccctc
      301 gatctcgcga cgcgccggcg cgtgcacttc atcccggtcc tcggcatcgc gctgctcgtc
      361 gtcgcgttct tcaaggtgcc gctctattcg gcggcgatct ggcgcgaggt cggcagcgca
      421 ttgctgcggc cgttcgtttg acggtcttcc acctcgcgtg gacctggcct aagatctaag
      481 tcgattctgg accgaggggt ggccggcgac ggcctgagag atcgacgtcg agtcgatcaa
      541 ccctttgaac ctgatccggt tagtaccggc gaagggacgg gaaattccga tcttccccgc
      601 tcgctagttg aaccgggtct cgctcgtaga ggagatccga catgtcgttt cgtttcacgc
      661 gccgcacggc gctcgcgctc gtcgcggcgg cggcagctca cgcggcgctc ggtcagtccg
      721 agcgcgtcga ggagatcgtc gtcacggcgg actttcgcga gtcgcgcatc gaggagctgc
      781 cggtcagcgt cacggtgctc gaccgcgaag agctgaaggc cgcggcgcag cagcatttcg
      841 aggaagtcat ccgcagcgtc ccgaacctga atctctcggg agaaggctcg cgcgcccgtt
      901 attttcagct gcgcggcgtc ggcgagctcg agcagtacga gggcgcgccg aacccgtcga
      961 tcggcttcat cgtcgacgac atcgatttct cgtcgctcgg cagcgccgcg accctgttcg
     1021 acgtcgatcg agtcgaagtg ctgcgcggtc cgcaaggcac gcgctacggc gcgaacgcgc
     1081 tcggcggcct cgtctacatg cgctcgcagg cgccgacccc gacgttgtcg gccgacctcg
     1141 aagcgacggc cggcagcgac ggcacggacg cgctcggcgc cgcgttcggc ggtccgatcg
     1201 gcgacgcgct cggcttcagg acgtcgttgc agacctaccg gaacaacggt tttcgcgaca
     1261 acgtttacct cggccgcgac gacacgtacg gccgcgacga gctgaccgcg cgcggcaagc
     1321 tcgcgtggtc gccgagcgac gacgtgcaag tcgatttcac cgggctctac gtcgacgtcg
     1381 acaacggcta cgacgcctgg gcgatcgaca acggtttcaa tacgtactcg gacgatccgg
     1441 gccgcgacgc gcagcagtcg actgcgggct cggtgcgcgt gcaggctgcg ctcgcgcgcg
     1501 tcgatctcgt gagcattacg ggcttcgccg acacggacgc ggtgttcagc ttcgatgcgg
     1561 actggggcaa tgccggattc tgggcgccgt atgtttatga ctacttcacc gcgaacgatc
     1621 gcgagcggcg cacggcgagc caggagctgc gcgtgctctc gaagcccggc gcgatcgcaa
     1681 gcggccgcgg cgagtggctc gcgggcgtct atgtgctcga cctcgaggag ggcaacgacc
     1741 agaccacgac cggcgtctac gaggatccgt tctgcgggcc ggcctgctcg ctgctgaccg
     1801 acacggcagt gcgcagcgac tacgacgcga cgaacctcgc cttgttcggc caagtcgcgt
     1861 tcgacttgaa cgaccggctc ggtttgacgg cgggcctgcg ctgggagcgg cgcagcgccg
     1921 actcctcgga cacgagctcg aacgccttct cgcccgacga cgacatgctc ggcggcgagc
     1981 tcgcgttcga ttggcagctc gccgagcgcc gctcggcgta cgtgcgtctc gcgcgcggct
     2041 acaaagccgg cggattcaac gtctcgctgg ccggtgtcga tttcggctcc gtcgacaacg
     2101 a
//
LOCUS       DASSNN010000247         4741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_281969, whole genome shotgun sequence.
ACCESSION   DASSNN010000247 DASSNN010000000
VERSION     DASSNN010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281969"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VFL84_04470"
     CDS             complement(<1..1059)
                     /locus_tag="VFL84_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HET7607909.1"
                     /translation="MNDLAKNVILWIVVAVVLWSVVSNFGVRSPRDTQTDYSTFLSWV
                     RDGSVKDVTFEGKTIKVEKTSGDHFVLYNPETSNDELIGLLDERGVKISGAPEKTPNL
                     LVSLFISSFPILLLIGVWIYFMRQMQGAGGGRGAMSFGKSRARLLGEDQVQVTFADVA
                     GVDEAKEEVVEIVEFLKDPGKFQRLGGKIPRGVLMVGSPGTGKTLLARAIAGEAKVPF
                     FTISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDER
                     EQTLNQLLVEMDGFEGNEGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDVRGREQ
                     ILKVHMRKVPVDGNVKPGV"
     gene            complement(1111..1734)
                     /locus_tag="VFL84_04475"
     CDS             complement(1111..1734)
                     /locus_tag="VFL84_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HET7607910.1"
                     /translation="MPRRTGSSQRWRQRQERDIYVEQASREGWRSRAVFKLEQIQAKE
                     KLLNRGMVCVDLGSAPGGFSQLAAKLVGPTGRVIAIDLLPMEPIPGVEFLQGDFMAPE
                     TLEALRNLVGPNPVELVMSDMAPNISGNRTIDQARSLALLDEALSFAREVLRPGGDFL
                     VKAFQGEGIDAFTRDLKREFRTVKTLKPKASRPESREIYLLARRFGM"
     gene            complement(1746..2222)
                     /gene="greA"
                     /locus_tag="VFL84_04480"
     CDS             complement(1746..2222)
                     /gene="greA"
                     /locus_tag="VFL84_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET7607911.1"
                     /translation="MSKVPLTARGAAQLKEELRRLKTEERPKITAAIATARAHGDLKE
                     NAEYHAAKEQQGLMEARIRDIEAKLGNAQIIDVTTVNAGGKVVFGSTVHLVDTSDGSE
                     AKYQIVGEDEADIKNGLLSIGSPIARALIGKKEGDEVDVSTPSGKRSYEIAAVEYI"
     gene            complement(2219..>4741)
                     /gene="carB"
                     /locus_tag="VFL84_04485"
     CDS             complement(2219..>4741)
                     /gene="carB"
                     /locus_tag="VFL84_04485"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET7607912.1"
                     /translation="ENFDPMGIHTGDSITVAPAQTLTDKEYQRMRDASIAVLRKIGVD
                     TGGSNVQFAVSPKDGRLLVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELK
                     NEITGGATPASFEPTIDYVVTKVPRFTFEKFPQAADRLTTQMKSVGEVMAIGRTFQES
                     LQKALRGLEIGTDGLNPIMKSVETEEDWAKLRHELRMAGSRRLWYVGDAFRHGLTLDE
                     VAELTAIDPWFLVQIKELIDIEKKVKEMGPEALMPEELRKLKRRGFSDHRIGDLIGVK
                     EKIVRTARYKENIRPVFKRVDSCAAEFASHTAYLYSTYEDFCEANPTDRQKIMILGGG
                     PNRIGQGIEFDYCCVHASFALKEAGFETIMVNCNPETVSTDYDSSDRLYFEPVTLEDV
                     LEIVHKEKPKGVIVQYGGQTPLKLARALEEAQVPIIGTSPDSIDLAEDRERFQQLVNK
                     LKLKQPPNRTARNEEEAVAKAKEIGYPLVVRPSYVLGGRAMEIVHGEDELRDYVERAV
                     SVSNDSPILLDRFLDLAVEVDVDAISDGEEVVIGGIMEHVEQAGIHSGDSGCSLPPFS
                     LTPEVQKALSDQVRQMAQALQVNGIMNTQFAIQNGVIYVLEVNPRASRTIPFVSKAIG
                     QPLAKIAAKAMAGISFKEQGFSVPGKRPYFSVKESVFPFNKFPDADPILGPEMKSTGE
                     VMGTGRTFGEAYAKAQFASGVVLPRRGLAFISVRDRDKPGVVELGKMLVARGFEIVAT
                     GGTAKALKDGGVPCQRVNKVREGRPHVVDMIKNRELSLIVNTTEGKQAIRESHSIRGE
                     AVHYKVTYYTTVAAAKATCDALDHLDNVVVNRLQDLHAEASV"
BASE COUNT          742 a         1589 c         1484 g          926 t
ORIGIN      
        1 gacgccgggc ttcacgttgc cgtcgacggg caccttgcgc atgtgaacct tgaggatctg
       61 ctcgcggccg cgcacgtccg gcaacggcac gacgacctga cggtcgaagc ggcccggccg
      121 cagcaacgcg gggtcgagca cgtcgggccg gttcgtggcc gcgatgacga tgacgccctc
      181 gttgccttcg aagccgtcca tctcgacgag cagctggttc agcgtctgct cgcgctcgtc
      241 atgaccgccg cccaagcctg cgccgcgatg gcggccgacc gcgtcgatct cgtcgatgaa
      301 gatgatgcac ggcgcgtgct tcttcgcctg ctcgaacatg tcgcgcacgc gcgaggcgcc
      361 gacgccgacg aacatctcga cgaagtccga gcccgagatc gtgaagaacg gcaccttggc
      421 ctcgccggcg atggcgcggg cgagcagcgt cttgccggtg ccgggcgagc cgaccatcag
      481 cacgccgcgc ggaatcttgc cgccgaggcg ctggaacttg ccggggtcct tgaggaactc
      541 gacgatctcg acgacctctt ccttcgcttc gtcgacaccg gcgacgtccg cgaacgtgac
      601 ctgcacctgg tcctcgccga gcagccgcgc gcggctcttg ccgaacgaca tggcgccgcg
      661 gccgccgccc gcgccctgca tctggcgcat gaaatagatc cagactccga tcaggagcag
      721 gatcgggaac gaggagatga acagcgagac gagcaagttc ggcgtcttct cgggcgcgcc
      781 ggagatcttg acgccgcgct cgtcgagcag accgatcagc tcgtcgttgc tcgtctcggg
      841 gttgtacaga acgaagtgat cgccgctcgt cttctcgacc ttgatcgtct tgccttcgaa
      901 cgtgacgtcc ttgaccgatc catcccgaac ccaggacaga aacgtcgaat aatccgtctg
      961 cgtatcgcgc gggctgcgca cgccgaagtt gctgacgacg gaccacagga ccaccgcaac
     1021 tacgatccag agaatgacgt tttttgcgag atcgttcaaa gctttacctc gttacggccg
     1081 tcctggccgc aagtatcctt tcagacccta ctacatcccg aagcgtctag ccagcaaata
     1141 aatctcgcgg ctctcgggcc tcgatgcttt gggtttcagg gttttcacgg tcctgaactc
     1201 ccttttgaga tcgcgagtga acgcatcgat tccctcgcct tggaatgcct tgaccaggaa
     1261 gtcgcctccg ggtcgcagca cctctcgcgc gaacgacagc gcttcatcga gcagcgccag
     1321 gctcctcgcc tgatcgatcg tgcgattacc gctgatattg ggagccatgt ctgacatcac
     1381 aagctcgaca gggttcgggc cgacgaggtt acgcagcgcc tcgagggttt cgggtgccat
     1441 gaagtctcct tgcaggaact cgacgcccgg gatcggctcc atcggcagca gatcgatcgc
     1501 gatgacgcgg ccggtcggac cgacgagctt ggcggcaagt tggctgaagc cgcccggcgc
     1561 ggagccgagg tcgacgcaga ccattccgcg gttgagcagc ttctccttcg cctggatctg
     1621 ctcgagcttg aagacggcgc gcgagcgcca accctcgcgg ctcgcttgct cgacatagat
     1681 gtcgcgctcc tggcgttgcc gccaacgctg gctcgaaccc gtgcgccgcg gcatcgcgcc
     1741 ggcctttaga tgtactcgac ggccgcgatc tcgtaggagc gcttgccgct cggcgtcgac
     1801 acgtcgactt cgtccccttc cttcttgccg atcagcgcgc gcgcgatcgg cgagccgatc
     1861 gagagcagcc cgttcttgat gtcggcctcg tcctcgccga cgatctgata cttggcctcg
     1921 ctgccgtcgg acgtgtcgac gagatgcacc gtcgagccga acacgacctt gccgccggcg
     1981 ttcacggtcg tgacgtcgat gatctgcgcg ttgccgagct tcgcctcgat gtcgcggatg
     2041 cgcgcctcca tgaggccttg ctgctccttc gcggcgtggt actcggcgtt ctccttgagg
     2101 tcgccgtgcg cgcgcgccgt cgcgattgcg gccgtgatct tcggccgctc ctcggtcttg
     2161 agcctgcgca gctcctcttt cagttgcgcg gcgccgcgcg ccgtcagggg caccttgctc
     2221 aaaccgacgc ctccgcgtgc aagtcttgca gcctattcac gacgacgttg tcgaggtggt
     2281 cgagggcgtc gcaagtcgct ttcgccgcgg cgaccgtcgt gtagtacgtc accttgtaat
     2341 gcacggcctc gccgcggatc gaatgcgatt cacggatcgc ttgcttgccc tcggtcgtgt
     2401 tcacgatcag ggatagctcg cggttcttga tcatgtcgac gacgtgcggg cgcccttcgc
     2461 gcaccttgtt gacgcgctgg cacggcacgc cgccgtcctt cagggctttc gccgtcccgc
     2521 ccgtcgcgac gatctcgaag ccgcgcgcga cgagcatctt gccgagctcg acgacgccgg
     2581 gcttgtcgcg atcgcgcacg ctgatgaacg cgagcccgcg ccgcggcagc acgacgcccg
     2641 aggcgaactg cgctttcgca tacgcttcgc caaaggttcg gccggtgccc atgacctcgc
     2701 ccgtcgattt catctcgggc cccaagatcg gatcggcgtc ggggaacttg ttgaacggga
     2761 acaccgactc cttgaccgag aagtacggcc gcttacccgg cacgctgaag ccctgctcct
     2821 tgaagctgat gccggccatc gccttcgcgg cgatcttcgc gagcggttgc ccgatcgctt
     2881 tcgacacgaa cggaatcgtg cgcgacgcgc gcgggttcac ctcgagcacg tagatgacgc
     2941 cgttctggat cgcgaactgc gtattcatga tgccgttgac ttgcagcgct tgcgccatct
     3001 gccggacctg gtcgctgagc gccttctgca cctcgggcgt caagctgaac ggcggcagcg
     3061 agcagccgga gtcgccggaa tgaatgccgg cctgctcgac gtgctccatg atgccgccga
     3121 tgacgacctc ctcgccgtcg gaaatggcgt cgacgtcgac ctcgacggcg agatcgagga
     3181 agcggtcgag caggatcggg ctgtcgttcg agacgctgac ggcgcgctcg acgtagtccc
     3241 gcagctcgtc ctcgccgtgc acgatctcca tcgcgcggcc gccgagcacg tacgagggcc
     3301 gcacgacgag cggataaccg atctctttcg ctttcgcgac ggcctcctcc tcgttgcgcg
     3361 ccgttcgatt cggcggctgc ttcagcttca gcttgttgac gagctgctgg aagcgctcgc
     3421 gatcctccgc gaggtcgatc gagtcgggcg acgtgccgat gatcggcact tgggcctcct
     3481 cgagcgcgcg cgcgagcttc agcggcgtct ggccgccgta ctgcacgatg acgcccttcg
     3541 gcttttcctt gtgcacgatc tcgagcacgt cctcgagcgt gaccggctcg aagtacaagc
     3601 gatcggagct gtcgtagtcg gtcgacaccg tctccgggtt gcagttgacc atgatcgtct
     3661 cgaacccggc ttccttgagc gcgaacgacg cgtgcacgca gcagtagtcg aactcgatcc
     3721 cctggccgat gcggttgggg ccgccgccca agatcatgat cttctgccga tcggtcggat
     3781 tggcttcgca gaagtcctcg taggtcgagt acaggtacgc cgtgtggctc gcgaactcgg
     3841 cggcgcagct gtccacgcgc ttaaagaccg gcctgatgtt ttctttgtaa cgtgctgttc
     3901 tgactatctt ttctttgacg ccgatcaggt cgccgatgcg gtggtcgctg aagccgcgcc
     3961 gcttgagctt gcgcagctcc tcgggcatca acgcctcggg acccatctcc ttgaccttct
     4021 tctcgatgtc gatgagctct ttgatctgca cgaggaacca cgggtcgatg gccgtgagct
     4081 cggccacctc gtcgagcgtc aagccatgcc ggaacgcgtc gccgacgtac cagagccggc
     4141 ggctgccggc catgcgcagc tcgtgcctga gcttggccca gtcctcttcg gtctcgaccg
     4201 acttcatgat cgggttgagc ccgtcggttc cgatctcgag cccgcgcagc gctttctgca
     4261 gcgactcctg gaacgtgcgg ccgatcgcca tgacctcgcc gacggacttc atctgcgtcg
     4321 tcaggcggtc ggcggcctgc ggaaactttt cgaacgtgaa gcgcggaacc ttcgtgacga
     4381 cgtagtcgat cgtcggctcg aacgacgccg gcgtcgcgcc gcccgtgatc tcgttcttga
     4441 gctcgtcgag cgtgtagccg acggcgagct tggccgcgac tttcgcgatc ggaaagcccg
     4501 tggccttaga cgcgagcgcc gacgagcgcg agacgcgcgg gttcatttcg atgacgagca
     4561 gccgcccgtc cttcggcgag accgcgaact gaacgttcga gccgccggtg tcgacgccga
     4621 tcttgcgcag cacggcgatc gacgcgtcgc gcatgcgctg atattccttg tccgtcagcg
     4681 tctgcgccgg cgccacggtg atcgagtcgc ccgtgtgaat gcccatggga tcgaaattct
     4741 c
//
LOCUS       DASSNN010000248         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1795210, whole genome shotgun sequence.
ACCESSION   DASSNN010000248 DASSNN010000000
VERSION     DASSNN010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795210"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2449)
                     /locus_tag="VFL84_04490"
     CDS             complement(<1..2449)
                     /locus_tag="VFL84_04490"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HET7607913.1"
                     /translation="MGKNLVIVESPAKARTIEKYLGSDFEVLASYGHVRDLVPKEGAV
                     DPEHGFAMKYQLIDKNEKHVDKIVKAAKKAAAVYLATDPDREGEAISWHVYEVLKERK
                     VIDSKPVQRVVFHEVTKRAIQEAIDQPRDLSEQLVNAQQARRALDYLVGFNLSPLLWK
                     KVRRGLSAGRVQSPALRLIVEREDEITAFKAEEYWTIEALAEQASQTFPARLSVFGRE
                     KVEQFTFTNEAAVRAAEQKIRDTAHGVLTVAKVDKKQRRRNPAAPFTTSTLQQEASRK
                     LGFSAQRTMTVAQRLYEGIDIGEGSVGLISYMRTDSVTLADEALTEIRSVITERYGAE
                     NLPEEPRRYTTKAKNAQEAHEAIRPTAAARHPDELRRFLDPNQYKLYDLIWKRTVASQ
                     MNPAVYDTVALDLAAGDSADSDISFRATGSVLVSPGFIAVYQEGQDDTSPDEQDRILP
                     AVAEGDRLTLKDVQSAQHFTEPPPRYSEATLVKALEEFGIGRPSTYASIISTLRNREY
                     VEMDNRRFIPTDVGRVVNRFLTQFFSQYVDYEFTARMEDDLDAVSRGEREWVPLLDEF
                     WKPFTKLVEHTDSNVTREQASQARVLGVDPASGRPVSVRMGRYGAFVQIGTKEDVEKP
                     RFAGLRPGQKMDTIELQAALDLFKLPRELGQTAEGEPVSANIGRFGPYVRYANKFVSI
                     KGDDPYTITLERALELIAEKKVADANRLILDFPDAGIQVLNGRYGPYITNKHKNAKIP
                     KDKAPKELTLEECQALLAAAPERRGRGMKRKPAPAAATAEAAAETAPAADAKAPAAKK
                     KKKKTKKKKRKAAASPKA"
BASE COUNT          356 a          819 c          835 g          526 t
ORIGIN      
        1 cggctttcgg tgacgccgcg gcttttctct tctttttctt ggtcttcttt ttcttctttt
       61 tcgcggccgg cgctttcgcg tcggctgcgg gcgccgtttc ggccgcggct tccgccgttg
      121 ccgctgccgg cgccggcttg cgcttcatgc cgcggccgcg ccgctcgggc gccgcggcaa
      181 gcagcgcctg gcattcctcg agtgtgagct ccttcggcgc cttgtctttc gggatcttgg
      241 cgttcttgtg tttgttcgtg atgtacggcc cgtagcggcc gttcagcact tggatgccgg
      301 cgtcggggaa gtcgaggatc agccggttcg cgtcggcgac cttcttctcg gcgatgagct
      361 cgagcgctcg ctcgagcgtg atcgtgtacg gatcgtcgcc cttgatcgag acgaacttgt
      421 tcgcgtagcg aacgtacggg ccgaaccggc cgatgttcgc ggaaacgggc tcgccttcgg
      481 ccgtctggcc cagctcgcgc ggcagcttga acagatcgag ggcagcttgc agctcgatcg
      541 tgtccatctt ctgcccgggc ctaagtcccg cgaaccgcgg cttctcgacg tcctctttcg
      601 tgccgatctg cacgaatgcg ccgtaccgcc ccatgcgcac gctgacgggg cggccgctcg
      661 cgggatctac gccaagcacg cgcgcttgcg aagcctgctc gcgcgtgacg ttgctgtcgg
      721 tgtgctcgac gagcttcgtg aacggcttcc agaactcgtc gagcagcggc acccactcgc
      781 gctcgccgcg cgacacggcg tcgaggtcgt cctccatgcg cgccgtgaac tcgtagtcga
      841 cgtactgcga gaagaactgc gtaaggaatc ggttgacgac gcgcccgacg tcggtcggga
      901 tgaatcgccg attgtccatc tcgacgtact cgcgattgcg cagcgtcgag atgatcgacg
      961 catatgtcga cgggcggccg atgccgaatt cctcgagagc cttgacgagc gtggcctccg
     1021 agtagcgcgg cggcggctcc gtgaaatgct gcgcgctctg cacgtccttc aaagtcaacc
     1081 gatcgccttc ggccaccgcg ggcaagatgc ggtcctgctc gtcggggctc gtgtcgtcct
     1141 ggccttcctg gtagacggcg atgaacccgg ggctcacgag aacggagccc gtcgcgcgga
     1201 acgaaatgtc gctgtcggcg gagtcgccgg ccgcgagatc gagcgcgacg gtgtcgtaga
     1261 cggcgggatt catctgcgac gcgacggtgc gcttccagat gaggtcgtag agcttgtact
     1321 gattcgggtc gaggaaccgg cgcagctcgt ccggatgccg cgcggcggcg gtcgggcgaa
     1381 tcgcttcgtg cgcttcctgc gcgttcttcg ccttcgtcgt gtagcggcgc ggctcctcgg
     1441 gcaaattctc ggcgccgtaa cgctcggtga tgacgctgcg gatctcggtc agcgcctcgt
     1501 cggcgagcgt cacggagtcc gtgcgcatgt agctgatcaa gccgacggag ccttcgccga
     1561 tgtcgatgcc ctcgtacagg cgctgcgcca cggtcatcgt gcgctgcgcg ctgaagccga
     1621 gcttgcgcga agcttcctgc tgcagcgtcg aggtcgtgaa cggcgccgcc ggattgcggc
     1681 ggcgctgctt cttgtcgact ttcgcgaccg tcagcactcc gtgcgccgta tcgcgaatct
     1741 tctgctcggc ggctcgaacc gcggcctcgt tcgtgaacgt gaactgctcg accttctcgc
     1801 ggccgaacac ggacagccga gccgggaacg tctggctcgc ctgctcggcc agcgcctcga
     1861 tcgtccagta ctcctccgcc ttgaaggccg tgatctcgtc ctcgcgctcg acgatgagcc
     1921 gaagcgcggg gctctgcacg cggcccgccg agagcccgcg gcggaccttc ttccacaaga
     1981 gcggcgagag attgaaaccg acgagatagt cgagcgctct gcgcgcttgc tgcgcgttga
     2041 cgagctgctc ggacaagtcg cgcggctggt cgatcgcttc ctggatcgcg cgcttcgtga
     2101 cctcgtggaa gacgacgcgc tgcacgggct tcgagtcgat gaccttgcgt tccttgagca
     2161 cctcgtacac gtgccaagaa atcgcctctc cttcgcggtc ggggtcggtc gcgagataga
     2221 cggccgcggc ctttttcgcg gccttcacga tcttgtcgac gtgcttctcg ttcttgtcga
     2281 tgagctgata tttcatcgca aagccgtgct cgggatcgac cgcgccttcc ttcggcacga
     2341 ggtcgcgcac gtggccgtac gacgcgagca cctcgaaatc gctaccgagg tatttctcga
     2401 tggtgcgcgc cttggccggc gattcgacga tgacgaggtt cttgcccatg gcgagcgctg
     2461 cgatggccta cggtaatctc acgtgcgggc gcatgggttt tcgagggagc ctcattaaat
     2521 agagcaacgg cagagc
//
LOCUS       DASSNN010000249         3386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1057808, whole genome shotgun sequence.
ACCESSION   DASSNN010000249 DASSNN010000000
VERSION     DASSNN010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057808"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VFL84_04495"
     CDS             complement(<1..458)
                     /locus_tag="VFL84_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7607914.1"
                     /translation="MKTRGRGEMHGTLLIARLTWLETRRRRIALAALICGLLFLAVFG
                     TAMYFGFDIAEMEGAAGIEMRIAATILTVAGLYVVNFLVTAVAILLPIDSLSGEIGSG
                     VIQTLASKPVDRAAIVVGKWLAYLLMTAAYLALMAGGIFLIMRVFADYTAP"
     gene            complement(436..1353)
                     /locus_tag="VFL84_04500"
     CDS             complement(436..1353)
                     /locus_tag="VFL84_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7607915.1"
                     /translation="MPEYAIETAALRREFGRNVAVAGLSLAVPRGEVFGFLGPNGAGK
                     TTSLKLLLGLVAPTSGNGSVLGAPLGNTAVRARIGFLPEHFRFHDCLNARELLHFHGR
                     LLGLPARGLGTRIEALLLRVGLDDAADRPLRTYSKGMTQRAGLAAALLGSPELVFFDE
                     PTSGLDPLGRLLVRDVINELRDNGATVFLNSHLLGEVEATCDRVAFVKNGLVLHELEL
                     KVATGTLDVELRVGHVDDALLEGLAAFGTGVTRDETLVRMHANSDGMLPELARWLVGR
                     GVALYELRCRRKTLEEWFVEIMGEDQRPG"
     gene            complement(1354..2334)
                     /locus_tag="VFL84_04505"
     CDS             complement(1354..2334)
                     /locus_tag="VFL84_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607916.1"
                     /translation="MDDQFLRDLRAQPNSEFAARLRATLRTVPPHAVARVAPAVRFRK
                     WFAAAASITVVGFAFTLPPVQAAAEAFLDLFRVRQFTGVEFDPAQLATRLQGLESSGI
                     SPESIFGNVEPLTDFESQPVSYATAAEAGQAAGIRVRTPAWLPNGYSSTGVMVSAEHA
                     ARITVNTEGLQAVLDALGLADVELPQGLDGETVTVRVPPIVSQSFASGDRTALVIQAK
                     SPDMTFPAGLDLSKLAYAGLRVLGMSRDEAYRMSVTIDWRSTLIVPVPAKAMAYRPIN
                     VSGNEGLLISGLTDNDSPGGVLMWSSGEQTYAVAGALSGEELLEIAQNLQ"
     gene            complement(2348..2878)
                     /locus_tag="VFL84_04510"
     CDS             complement(2348..2878)
                     /locus_tag="VFL84_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7607917.1"
                     /translation="MATEGEVLERPFLGVVAAEPSWDAVYTEQLPRVYNFFRYRLGND
                     AIAEDLTSRTFEKAWAARSRYRRDLAGFSTWLISIARNVATDHLRAQRPHLPLEAAED
                     VAARGTPHDDASNESDLARLSALLEKLPQREQEIVALKYGAEATNRAIAELTGLSESN
                     VGTILHRVVQALRRDW"
     gene            3111..>3386
                     /locus_tag="VFL84_04515"
     CDS             3111..>3386
                     /locus_tag="VFL84_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607918.1"
                     /translation="MNDIRLAARQVVRYPGFSAVVIATLALGIGACTLIFSAVNGVLL
                     KPLPYPEPERIVRVRQIGRTGIAADNISGPNFSDLQESARSFAALARY"
BASE COUNT          597 a         1146 c         1110 g          533 t
ORIGIN      
        1 ggcgccgtgt agtccgcgaa tacgcgcatg atcagaaaga tgccgcctgc catcagagcg
       61 agatacgcgg ccgtcatcag caaatacgcg agccacttgc cgacgacgat cgcggcgcgg
      121 tcgaccggtt tcgacgcgag cgtctgaatg acgcccgagc cgatctcgcc ggacaacgaa
      181 tcgatcggca gcaggatcgc gacggccgtc accaggaaat tgacgacgta gagacccgcc
      241 accgtcaata tcgtcgcggc gatgcgcatc tcgatgcccg ccgcgccttc catctcggcg
      301 atgtcgaagc cgaagtacat cgccgtcccg aacacggcca ggaacaggag gccgcagatc
      361 agcgccgcga gcgcgattct tcggcggcgc gtctcgagcc aggtgagtcg agcgatcagc
      421 agcgtgccgt gcatctcacc ccggcctctg gtcttcaccc atgatctcga cgaaccactc
      481 ctcgagcgtc ttgcggcgac agcgaagctc gtagagcgcg acgccgcggc cgacgagcca
      541 gcgcgcgagc tccggcagca tgccatccga attcgcgtgc atgcgcacga gtgtctcgtc
      601 gcgcgtgaca ccggtgccga acgcggccag cccttcgagc aacgcgtcgt cgacgtgacc
      661 gacgcgcagc tcgacgtcga gcgtacccgt cgctactttg agctcgagct catgcaagac
      721 gagcccgttc ttgacgaacg cgacgcggtc gcacgtcgcc tcgacctcgc cgagcaagtg
      781 cgagttcaag aacacggttg cgccgttgtc gcgcagctcg ttgatgacgt cgcgcacgag
      841 caagcggccg agcggatcga gccccgaggt cggctcgtcg aagaacacga gctcgggcga
      901 gccgagcagc gcggccgcga gccccgcgcg ctgcgtcatg cctttgctgt acgtgcgcag
      961 cgggcggtcc gctgcgtcgt cgagaccgac gcgcagtagc aatgcttcga tgcgcgtgcc
     1021 gagcccgcgc gcgggcaacc ccagcaagcg tccatgaaaa tgcagcagct cgcgcgcgtt
     1081 caggcagtcg tgaaaacgaa agtgctcggg caggaagccg atgcgtgcgc gcacggcggt
     1141 gttgccgagc ggcgcgccga gcacgctgcc gttaccggac gtcggcgcga cgagaccgag
     1201 caataatttc aacgacgtcg tcttgccggc gccgttcggc cccagaaagc cgaacacctc
     1261 gccgcgcgga acggcgaggg acagacccgc taccgcgacg ttgcggccga actcgcgccg
     1321 caacgccgcg gtttcgattg cgtattccgg catctattgg agattctgcg cgatctccag
     1381 cagttcttca ccgctcaaag cccccgcgac tgcgtaggtc tgctcgccgg acgaccacat
     1441 cagcacgccg ccgggcgagt cgttgtccgt gagacccgag atcaagagtc cctcgttgcc
     1501 ggacacgttg atcggacgat aggccatcgc cttcgcgggg accggcacga tcaacgtcga
     1561 acgccagtcg atcgtgacgg acatgcgata cgcctcgtcg cgggacatgc cgagcactcg
     1621 caggcccgcg taggcgagct tcgagagatc gagcccggcc gggaacgtca tgtccggact
     1681 ctttgcttgg atcacgagcg ccgtgcgatc gccgctcgcg aagctctgac tcacgatcgg
     1741 cggcactcgc acggtgaccg tttcgccgtc gaggccctgc ggcagctcga cgtcggcgag
     1801 cccgagcgcg tcgagcacgg cctgaagccc ctccgtattg acggtgatgc gtgcggcatg
     1861 ctcggccgac accatgacgc ctgtgctcga gtaaccgttc ggcagccacg ccggcgtgcg
     1921 cacacggatc ccggcggcct gaccggcttc cgccgcggtc gcatacgaga cgggctggct
     1981 ctcgaagtcc gtcagcggct ccacgttgcc gaagatcgac tcggggctga ttccgctcga
     2041 ctccaggccc tggaggcgcg tcgcgagctg cgccgggtcg aattccacgc ccgtgaactg
     2101 cctgacccgg aacaagtcta gaaacgcttc ggccgccgcc tgcaccggcg gcagcgtgaa
     2161 cgcaaagccc acgacggtga tcgacgccgc ggccgcgaac cacttgcgaa accggaccgc
     2221 aggcgcgacg cgcgcgacgg cgtggggagg gaccgtgcgc aacgtcgcgc gcaatcgcgc
     2281 tgcgaattcc gaattcggct gtgctctcag atcgcgcaag aattggtcgt ccattaacga
     2341 aacctcttta ccagtcgcgg cgcaacgctt gcaccacgcg atgcaagatc gtgccgacat
     2401 tcgattcgct caaacccgtg agctcggcga tggcgcggtt cgtcgcttcg gcaccgtact
     2461 tcagcgcgac gatctcctgc tcgcgttgcg ggagcttctc gagaagcgcg ctcaagcggg
     2521 cgagatccga ttcgttcgaa gcgtcgtcgt gcggcgtgcc gcgcgccgcg acgtcttccg
     2581 ccgcctcgag cggcagatgc ggccgctgcg cgcgcagatg atccgtcgcg acgttgcggg
     2641 cgatgctgat cagccaggtc gagaagcccg cgagatcgcg ccgataacgc gaacgcgcgg
     2701 cccacgcttt ctcgaacgtg cgcgacgtca agtcttccgc gatcgcgtcg ttgcccaagc
     2761 gatagcggaa gaagttgtag acgcgcggca gctgctccgt gtaaaccgcg tcccagctgg
     2821 gctccgcggc tacgacgccc aggaaaggcc gctccagaac ttcgccctcg gttgccatcg
     2881 cagcaatata cgggtgcccg gtgcgagtat cacaggcccg cgcggcgctc gaccgcgacg
     2941 accgcccggt tatgggaaag gcagtggcgg aatcgggacg ccgacacgtg cctgtccggc
     3001 cagaagagcg ctgttttggc cgcccagcgt actggcatga tgcttgctcg cgagagcgca
     3061 acggctgggt tacgttgcct tcggcgcggc gtgatatcgg ggggcatgcg atgaacgata
     3121 ttcgtttggc cgcacgtcag gtcgtgcgct acccggggtt cagcgcggtc gtcatcgcaa
     3181 cgctggcact cggcatcggc gcttgcacgc tgatcttcag cgccgtgaac ggggtgctgc
     3241 tgaaaccgct gccgtacccc gagcccgagc ggatcgtccg cgtgcggcag atcggcagga
     3301 ccggcatcgc ggccgacaac atctcggggc cgaacttcag cgacttgcag gagtcggctc
     3361 ggtcgttcgc ggcgctggct cgctac
//
LOCUS       DASSNN010000250         2922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_787664, whole genome shotgun sequence.
ACCESSION   DASSNN010000250 DASSNN010000000
VERSION     DASSNN010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787664"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFL84_04520"
     CDS             <1..410
                     /locus_tag="VFL84_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HET7607919.1"
                     /translation="FLGLSQPSLNIAGGVLLFIIALRMIFPAAAGEVATEKDEPFIVP
                     LAMPMIAGPSTIAILLLLSSTEPERIWEWCVALVIAWAASTVILTASPFLLRVLGDRG
                     LRALERLMGMLLVLLATQLLLNGVREFVQSLDA"
     gene            complement(492..2309)
                     /locus_tag="VFL84_04525"
     CDS             complement(492..2309)
                     /locus_tag="VFL84_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7607920.1"
                     /translation="MAAKLDKTRQRGKLRIGDDWNAIRIIALSQSNPLKAIAEFVENS
                     IDARAKTVTITRGREHGEHYLAVRDDGTGVPRDAAGVPDFKYVATHICDSIKRRLKAE
                     GNAAGLQGEFGIGLLSFWTIGETMTMTSTGADQRAYQMTMRKGDPGFDVAQRRSLFAE
                     QGTELKVSPLLEGIRALSGEKLQWYLASELRDRIRSAGVRVTIIDKIARKQYEVEPRQ
                     FEGRLLHQLPAVRTPFGEAYTELYLTEPRDGAQVALTRLGTRVLADIRSLPDLDHEPW
                     SSNYLQGIVDAPFLNLTPGTRSGIIHDERYAALLAALEPLEAALRDLIADQRRAEEEQ
                     ASQQALRAIQRAFQEAMLALPPEEYDWFDVHGRARRAGPASGESTAGEVAEQSINIEE
                     PSSPETAQRQFFEFAGPLFSVVVSPASSTVLCNESRKLRALPRDRSRRRVTEDLVFSW
                     QILEGEGALSAVADQEVEFRAPGTPGLVRLAVTVTQREIVCTAEALITVTDRLERQGE
                     GAPLASARGLPGYTFERAAGELWRSRFDAERNIIVVNSGHRDFVFATRNRALQLRYLV
                     RLYVKELVLHNFAGVPGEQLLERMIELSLYAEEKLKPGG"
     gene            complement(2365..2673)
                     /locus_tag="VFL84_04530"
     CDS             complement(2365..2673)
                     /locus_tag="VFL84_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HET7607921.1"
                     /translation="MLELRPTCENCNQALPPDSLEARICTYECTFCASCVETVLDNVC
                     PNCGGGFTPRPIRPKKNWKGDNFLGKDPASTKVRHRPVDRAEHERFSAAIKRIPPHER
                     "
     gene            complement(2707..>2922)
                     /locus_tag="VFL84_04535"
     CDS             complement(2707..>2922)
                     /locus_tag="VFL84_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET7607922.1"
                     /translation="SHAVIGRYCLVGAGALITERKQFADRSLIIGAPARRVRELSDDE
                     CRGLEASAANYAERGRQYRRELAERAR"
BASE COUNT          415 a          988 c          978 g          541 t
ORIGIN      
        1 acttcctcgg cctgtcgcaa ccgtcgctga acatcgccgg cggcgtgctg ctgttcatca
       61 tcgcgctgcg catgatcttc ccggctgcgg ccggcgaggt cgcgacggaa aaggacgagc
      121 cgttcatcgt cccgctcgcg atgccgatga tcgcgggccc gtcgacgatc gcgatcctgc
      181 tgctgctgtc ctcgacggag cccgagcgca tttgggagtg gtgcgtggcg ctcgtaatcg
      241 cctgggccgc ctcgaccgtg atcctcacgg cgtcgccgtt cctgctgcgc gtgctcggcg
      301 atcgcggctt gcgcgcgctc gagcggctca tgggcatgct gctcgtgctg ctcgcgacgc
      361 agctgctctt gaacggcgtg cgcgagttcg tgcagtcgct cgacgcctaa gcgcgggttc
      421 ttgacctcgg ggaaaacgca acttggagcg cgcttccgcg gacatggacc gatactgcgt
      481 tcccgaccga cttagccgcc gggctttagc ttctcctccg cgtacagcga cagctcgatc
      541 atgcgctcga gcagctgttc gccgggaacg cccgcgaagt tgtgcagcac gagctctttc
      601 acgtagagcc gaacgagata gcgcaactgc agcgcgcgat tgcgcgtcgc gaacacgaag
      661 tcgcgatggc cgctgttgac gacgatgatg ttgcgctcgg cgtcgaagcg gctgcgccag
      721 agctcgccag ccgcccgctc gaacgtgtag ccgggcaggc cgcgtgcgct cgcgagcggc
      781 gcgccttcgc cttgccgctc gaggcggtcc gtcacggtga tcagtgcctc ggcggtgcag
      841 acgatctccc tctgcgtgac ggtcacggca aggcgaacga gccccggcgt gcccggcgcg
      901 cgaaactcga cttcttggtc ggcaacggcg gaaagcgccc cttcgccttc gagaatctgc
      961 cacgagaaca cgaggtcttc ggttacgcgg cgtcgcgacc tgtcgcgggg caacgcgcgg
     1021 agcttgcgcg actcgttgca gagcaccgtg ctcgaggccg gcgagacgac gacggagaag
     1081 agcgggcccg cgaactcgaa gaactgccgt tgagcggtct ccgggctcga cggttcctcg
     1141 atgttgatgc tttgctccgc aacctcgccg gccgtgcttt cgccgctcgc gggccccgcg
     1201 cgccgagcac ggccgtggac atcgaaccag tcgtactcct cgggcggcag cgccagcatc
     1261 gcttcctgaa acgctctttg aatggcgcgc agcgcctgct ggctcgcttg ctcctcttcg
     1321 gcgcgccgtt ggtctgcgat caagtcgcgt agcgccgcct cgagcggctc gagcgccgcg
     1381 agcagcgccg cgtagcgttc gtcgtgaatg atgccgctgc gcgttcccgg cgtcaggttc
     1441 aggaacgggg cgtcgacgat tccttgcaga taattcgagc tccacggctc gtggtcgagg
     1501 tcaggcaggc tgcggatgtc cgcgagcacg cgcgtcccta agcgcgtcaa cgccacttgt
     1561 gcgccgtcgc gcggctccgt cagatacagc tccgtgtacg cctcgccgaa cggcgtgcgc
     1621 acggccggca gctgatgcaa gagccgcccc tcgaactggc gcggctcgac ctcgtactgc
     1681 ttgcgcgcga tcttgtcgat gatcgtcacg cgcacgcccg cgctgcggat ccggtcgcgc
     1741 agctcggaag cgagatacca ctggagcttc tcgccgctca gcgcgcggat gccttcgagc
     1801 agcgggctca ccttgagctc cgtgccttgc tcggcgaaca gcgagcggcg ctgggccacg
     1861 tcgaaacccg ggtcgccctt gcgcatcgtc atctggtagg cgcgttgatc ggcgcccgtc
     1921 gacgtcatcg tcatcgtctc gccgatcgtc caaaagctca ggagcccgat gccgaattcg
     1981 ccctgaagtc ccgcggcatt gccttcggcc ttgagccggc gtttgatcga atcgcagatg
     2041 tgcgtggcga cgtacttgaa gtcgggaacg ccggctgcgt cgcgcggaac accggtaccg
     2101 tcgtcgcgga ccgccagata gtgctcgcca tgctcgcggc cgcgcgtgat cgtgaccgtc
     2161 ttggcgcggg cgtcgatgct gttctcgacg aactccgcaa tcgccttgag cggattgctc
     2221 tgcgacagcg cgatgatgcg gatcgcgttc cagtcgtcgc cgatccgcag cttgccgcgt
     2281 tggcgggtct tgtcgagttt tgcagccatc ttgcgtccgc tgcgcccagc ctagtgcaga
     2341 acgtacgccg gcgcagcgcc caggttagcg ttcgtgcggc gggatgcgtt tgatcgcggc
     2401 cgagaaccgt tcatgctcgg cgcggtcgac gggccgatgc ctgaccttcg tgctcgcggg
     2461 atccttgccg aggaagttat cgcccttcca gttctttttc ggcctgatcg gccgcggcgt
     2521 gaaaccgccg ccgcagttgg gacagacgtt gtcgagcacc gtttcgacgc agctagcgca
     2581 gaacgtgcac tcgtaggtgc aaatccgcgc ctcgagcgag tcgggcggga gcgcctggtt
     2641 gcagttctcg catgtcgggc gcagctcgag catcgcgcgc tccttttgga ttgcgagcca
     2701 gcgcggctag cgcgctcgct cggcgagctc gcgccgatat tggcggccgc gctcggcata
     2761 attcgcggcg ctcgcttcga gccctcggca ctcgtcgtcg ctgagctcgc ggacgcgccg
     2821 cgccggcgcg ccgatgatca gcgagcggtc ggcgaattgc ttgcgctccg tgatcagcgc
     2881 gcccgcgccg accaagcagt acctgccgat cacggcgtgg ct
//
LOCUS       DASSNN010000251         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_282254, whole genome shotgun sequence.
ACCESSION   DASSNN010000251 DASSNN010000000
VERSION     DASSNN010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282254"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1326)
                     /locus_tag="VFL84_04540"
     CDS             complement(235..1326)
                     /locus_tag="VFL84_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607923.1"
                     /translation="MNLRRLGSIVVKELRQLRRDRLTFAMIIGIPTMQLILFGYAINL
                     DVRNLETAVLDQSATARSRELIAGLAATGVMHFGHVVSSPQEVEALMRAGEISAAVVL
                     PPDFETRLERRDRPAVQIVVDGSEQVIQAAARQLAAFPVHGNEGWTTATSAVEVVNFY
                     NPQRRAAVNTVPGLVGMILTMTMVLFTAIALVRERERGNLEMLIATPVSPWELTVGKI
                     LPFIGIGLVQTTVVLVLGDVLFDVPLRGSVLELYLAALVFITANLSLGITISTLTRTQ
                     FQAMQMSFFTFLPQILLSGFMFPFAGMPKGAQYVAEALPLTHFLRLVRGIMLRGADLT
                     ELWRSLLALAIFIAIMLAVAVSRVRKRLD"
     gene            complement(1313..2242)
                     /locus_tag="VFL84_04545"
     CDS             complement(1313..2242)
                     /locus_tag="VFL84_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7607924.1"
                     /translation="MRATGLTKRFGPVVAVDHLDLTVRRREVYGFLGPNGSGKSTTIR
                     MLCGLLNPTSGDIDVLGHQIPRDAEALKRRIGYMTQRFSLYEDLTVDENLSFLASIHD
                     MPRAQARPRIDELVAKFWLTRQRKQLAGTLSGGQKQRLALAGTVLHKPDLLFLDEPTS
                     AVDPQSRREFWDSLFELADQGTTLLVSTHYMDEAERCTRLAILDFGRIVADGSPRELL
                     AGLPGRTWLVTPNEPRRAQHALRDLEGVIAVAQIGTSLRVLGSANGDLKQRIEARLRD
                     ANVEGTVQPTAPNLEDVFVAATIKPEDRARESA"
BASE COUNT          398 a          798 c          823 g          357 t
ORIGIN      
        1 cgacgcgtaa atcgcgccat ccggctgcgc tcgccccgcc cgcgagcacg acagccaaaa
       61 cggcaactgt gaccttcagc attggcgtct cccgtttgcg gccttgaatg ccttctcggc
      121 cgaacagtac gtgagccagg atagtcctgg tcgtccgcgt ttgcacgtgc tgatttgcca
      181 aaaccagccc tggcactcgg tccgcccggg cctatcgaat caaagggtta gcactcagtc
      241 gaggcgcttg cgcactcgcg acacggcgac ggcgagcatg atcgcgatga agatcgcgag
      301 cgcgagcagc gaccgccaga gctccgtgag gtcggcgccg cgcagcatga tgccgcgcac
      361 gagccgcaag aaatgcgtca gcggcagcgc ctcggcgacg tactgcgcac ccttcggcat
      421 gccggcgaac gggaacatga agcccgacaa caggatctgc ggcaggaacg tgaagaacga
      481 catctgcatc gcctggaact gcgtgcgcgt cagcgtcgag atcgtgatgc cgagcgacag
      541 gttcgccgtg atgaacacga gcgcggcgag atagagctcg agcacggagc cgcgcagcgg
      601 cacgtcgaac agcacgtcgc cgagcacgag cacgacggtc gtttgcacga ggccgatgcc
      661 gatgaacggc aggatcttgc cgaccgtgag ctcccacggg cttacgggcg tcgcgatcag
      721 catctcgaga ttgccgcgtt cgcgctcgcg cacgagcgcg atcgcggtga acagcaccat
      781 cgtcatcgtc aggatcatgc cgacgaggcc gggcacggtg ttgacggccg cgcgccgctg
      841 cgggttgtag aaattcacga cctcgaccgc gctcgtcgcg gtcgtccagc cttcgttgcc
      901 gtgcacgggg aacgcggcga gctggcgggc cgcggcctga atgacctgct cggagccgtc
      961 gacgacgatc tgcacggcgg gccggtcgcg ccgctcgagc cgcgtctcga aatcgggcgg
     1021 cagcacgacc gccgcgctga tctcgccggc gcgcatcagc gcttcgacct cctgcgggct
     1081 cgacaccacg tgcccgaaat gcatgacgcc ggtcgcggcg agccccgcga tgagctcgcg
     1141 cgagcgcgcc gtggcggatt gatcgagcac ggccgtctcg aggttgcgca cgtcgagatt
     1201 gatcgcgtag ccgaacagga tcagctgcat cgtcggaatg ccgatgatca tcgcgaacgt
     1261 gagccggtcg cggcggagct gccgcagctc cttgacgacg atcgagccga gcctacgcag
     1321 attcacgcgc ccggtcctct ggcttgatcg tggccgcgac gaacacgtcc tcgaggttcg
     1381 gcgccgtcgg ctgcacggtg ccttcgacat tggcatcgcg caggcgcgcc tcgatgcgct
     1441 gcttcaagtc gccgttcgcg ctgcccagca cacgcagcga cgtgccgatc tgcgcgacgg
     1501 cgatgacgcc ttcgaggtcg cgcagcgcat gctgcgcgcg acgcggctcg ttcggcgtga
     1561 cgagccaggt gcggccgggc agcccggcga gcagctcgcg cggcgagccg tcggcgacga
     1621 tccggccgaa gtcgaggatc gcgagccgcg tgcagcgctc ggcctcgtcc atgtagtgcg
     1681 tcgagacgag cagcgtcgtg ccctgatcgg cgagctcgaa cagcgagtcc cagaactcgc
     1741 gccgcgactg cgggtcgacg gcgctcgtcg gctcgtcgag gaacaacaga tccggcttgt
     1801 gcagcacggt gccggcgagc gcgagccgct gcttctggcc gccgctcaaa gtgcctgcga
     1861 gctgcttgcg ttgccgcgtc agccagaact tcgcgacgag ctcgtcaatc cgcggccgcg
     1921 cttgcgcgcg cggcatgtcg tgaatcgaag cgaggaacga caggttctca tcgaccgtca
     1981 ggtcctcgta gagggagaag cgctgggtca tgtagccgat gcgccgcttc agcgcctccg
     2041 cgtcgcgcgg gatttggtgg ccgagcacgt cgatgtcgcc cgaggtcggg ttcaacaggc
     2101 cgcagagcat gcggatcgtc gtcgacttgc cggacccgtt cggcccgagg aagccgtaga
     2161 cctcgcgtcg gcgcaccgtg agatcgagat ggtcgacggc gacgacgggc ccgaagcgct
     2221 tcgtgagccc cgtcgcgcgg atcgcgatct cgccgttcat cactgcaccg gcacggccgt
     2281 caccggcagg ccggccggca ggtccgcgac gccggggccg ctgtcgagca agagctcggc
     2341 gcgatagacg agccgggtcg cgtcgtcgcc ggcgag
//
LOCUS       DASSNN010000252         4826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1669095, whole genome shotgun sequence.
ACCESSION   DASSNN010000252 DASSNN010000000
VERSION     DASSNN010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669095"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..2247)
                     /locus_tag="VFL84_04550"
     CDS             complement(31..2247)
                     /locus_tag="VFL84_04550"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019501837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HET7607925.1"
                     /translation="MPKIIYTLTDEAPALATYSLLPIVQAFTTAAGCAVETRDISLAA
                     RILAQFPERLGARQTVGDDLKELGEIAKTPEANIIKLPNISASVPQLQAAIKELQQQG
                     YDVPDYPEEPGNDAEKDVKARYARVLGSAVNPVLREGNSDRRVAASVKQYAKKHPHSM
                     GRWSKDSKTHVASMGGGDFYSSEQSAVVQKSGKVKIELTSNRGDKTILKDGIGVKEGD
                     VLAASTMNRRALRDFYAKQIADAKAKGVLLSLHLKATMMKVSDPIMFGHAVEVYYQDV
                     FSKHADTLKELGVEPNNGIGDLYTKIKALPDAERSAIEADLAGVYKVRPELAMVDSSK
                     GITNLHVPSDVIVDASMPAAIRASGQMWGPDGKLHDTKAMIPDRCYAGIYQEVIDDCR
                     KNGAFDVPTMGNVSNVGLMAQAAEEYGSHDKTFEIPVSGTVRVLGADDKPLMEHRVEQ
                     GDIWRMCQTKDLPIRDWVKLAVNRARATGQPAIFWLDENRAYDRILIDKVGRYLRDHD
                     TSGLDIRIMSPVNAMRATLKRVRSGADTISVTGNVLRDYLTDLFPILELGTSAKMLSI
                     VPLLAGGGLYETGAGGSAPKHVQQFLKENYLRWDSLGEFLALAVSIEDYGTKTGNAAA
                     LVMAEALDAANGRFLESDKSPSRALGGIDNRGSHFYLAMYWARALADQTKDQALSARF
                     GKVAAALEAAEQDIVAELIGVQGKPVDIGGYYHPSPELTGRAMRPSPTLNGILAAV"
     gene            complement(2256..3149)
                     /locus_tag="VFL84_04555"
     CDS             complement(2256..3149)
                     /locus_tag="VFL84_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014109697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HET7607926.1"
                     /translation="MSTFDQQLAKIKNDKGFIAALDQSGGSTPKALKLYGIDESAWKD
                     EKGMYDIVHAMRTRIITSPAFNGKRLIGAILFEDTMDREIEGKPTPTYLWEVKGVVPF
                     LKVDKGLAEEQDGVQLMKPIPNLDALLKKAKAKGVFGTKMRSVIKQADPHGIEKVVKQ
                     QFDLAKKILAAGLVPIVEPEVDIKTPNKDQAEELLKAALLKELNALKPDQRVILKLTI
                     PSKDNLYADLIKHKNVLRVVALSGGYSREEANKRLARQHGMSASFSRALAEGLKAQQS
                     DEEFNAALDASIESIYRASIT"
     gene            3253..4662
                     /gene="dacB"
                     /locus_tag="VFL84_04560"
     CDS             3253..4662
                     /gene="dacB"
                     /locus_tag="VFL84_04560"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HET7607927.1"
                     /translation="MIGAAAATLAVHAGDLPPEIDRVLTGHGIPATDVSIVVQAVDDT
                     KPTLSHLADVPRSPASVMKTVTTWSALEYLGPAYTWPTEIYFLGAFDGRKLDGDLGIK
                     GYGDPQLVVEEVWKLLRALRRMGLTEISGDLVLDDSHFAVDEPDPGAFDGQPYRTYNV
                     VPSALLMNFKAVQFQFMADSARGRVNVATEPVLSNLKIDNNLTLVDGACAGYNAGISF
                     NHADPDELDHVVLDGKFSRRCNVYGLGRTVLQHDTFAFGLFSSLWKEVGGSFSGKMRK
                     AQIPLDATPALVWQSKPLGEVVRSINKNSNNVMTRQLLYTLGAEAVGVPGTRENGVAV
                     VRELLAARGLDTSSLMLQNGAGLSRDERASMQLLVDLLRAAYASPYAPEFIASLSLGG
                     LDGTTRGRFGRFDAAPASQVMHLKTGRLDHVSAVAGYVRAANGKTYVVAVVMNSPDAH
                     RGPGQELEEAVLRYTLALR"
BASE COUNT          737 a         1561 c         1577 g          951 t
ORIGIN      
        1 ttcggctacg cgggcagggt ttctttgcaa tcaaaccgcg gccaggatcc cgttcagcgt
       61 cggactcggc cgcatcgctc tgcccgtgag ctccgggctc gggtggtagt aaccgccgat
      121 gtcgacgggc ttgccctgca ccccgatgag ctcggccacg atgtcctgct cggccgcttc
      181 gagcgcggcc gcgaccttgc cgaaacgggc gctgagcgcc tgatctttcg tttggtccgc
      241 cagcgcgcgg gcccagtaca tcgcgagata gaagtggctg ccgcggttgt cgatgccgcc
      301 gagggcgcgt gaaggcgact tgtcgctctc gaggaagcgg ccgttcgcgg cgtcgagcgc
      361 ctcggccatg accagggccg ctgcgttgcc tgtcttcgtg ccgtagtcct cgatcgacac
      421 ggcgagggcc agaaactcgc cgagcgagtc ccagcgcaag tagttttcct tgaggaactg
      481 ctggacgtgc ttcggcgccg agccgccggc gcccgtttcg tagagaccgc cgcccgcgag
      541 cagcggcacg atcgagagca tcttggcgct cgtgccgagc tccaagatcg ggaagagatc
      601 cgtcaagtag tcgcgcagca cgttgcccgt caccgagatc gtgtcggcgc cgctgcgcac
      661 gcgcttcagc gtcgcgcgca tcgcgttgac gggcgacatg atcctgatgt cgagcccgct
      721 cgtgtcgtgg tcgcgcaaat accgcccgac cttgtcgatg aggatgcggt cgtaggcgcg
      781 gttctcgtcg agccagaaga tcgcgggctg ccccgtcgcg cgcgcgcggt tcacggcgag
      841 cttgacccag tcgcggatcg gaagatcctt cgtctggcac atgcgccaga tgtcgccctg
      901 ctcgacgcga tgctccatga gcggcttgtc gtcggcgccg agcacgcgga ccgtgccgct
      961 gacgggaatc tcgaacgtct tgtcgtgcga gccgtactcc tcggccgcct gggccatgag
     1021 gccgacgttc gagacgttgc ccatcgtcgg gacgtcgaat gcgccgttct tgcggcagtc
     1081 gtcgatgacc tcttgataga tgccggcgta gcagcggtcc gggatcatcg ccttggtgtc
     1141 gtgcagcttg ccgtccggcc cccacatttg gccggacgcg cgaatggcgg ccggcatcga
     1201 cgcgtcgacg atgacgtcgc tcggcacgtg caggttcgtg atgcccttgc tcgagtcgac
     1261 catcgcgagc tcgggccgca ctttgtagac gccggcgaga tccgcctcga tcgcggaacg
     1321 ctcggcgtcg ggcagtgcct tgatcttcgt gtagagatcg ccgatgccgt tattcggctc
     1381 gacgccgagc tccttcagcg tatcggcgtg cttcgagaag acgtcctggt aatagacctc
     1441 gacggcgtgg ccgaacatga tcgggtccga caccttcatc atcgtcgctt tcaagtgcag
     1501 cgacagcagc acgcctttgg ccttggcgtc ggcgatctgc ttcgcgtaga aatcgcgcag
     1561 cgcgcgacgg ttcatcgtcg acgcggcaag cacatcgcct tccttgacgc cgatgccgtc
     1621 cttgaggatc gtcttgtcgc cgcgattgct cgtgagctcg atcttgacct tgccgctctt
     1681 ctgcacgacg gccgactgct cgctcgaata gaaatctccg ccgcccatgc tcgcgacgtg
     1741 cgtcttcgag tccttgctcc agcggcccat cgagtgagga tgcttcttcg cgtattgctt
     1801 cacggaagcg gcgacgcggc gatccgagtt gccttcgcgc aagaccgggt tcacggcgct
     1861 gccgagcacg cgcgcatagc gcgccttgac gtccttctcg gcgtcgttgc cgggctcctc
     1921 ggggtagtcg ggaacgtcgt agccctgttg ctggagctcc ttgatcgcgg cctgaagctg
     1981 cggcaccgag gcgctgatgt tcggcagctt gatgatgttg gcttcgggcg ttttcgcgat
     2041 ctcgccgagc tccttgagat cgtcgccgac ggtttgccgt gcgccaagcc gctcgggaaa
     2101 ctgcgcgagg attcgcgcgg cgagcgagat gtcgcgtgtc tcgacggcgc agccggccgc
     2161 tgtcgtgaac gcctgcacga tcggcagcag cgaatacgtg gcgagcgccg gcgcctcgtc
     2221 cgtgagcgtg tagatgatct tgggcacgcg ggcggtcagg tgatcgaggc gcgatagatg
     2281 ctctcgatgg aggcatcgag ggcggcgttg aactcctcgt cggattgctg ggccttcagg
     2341 ccttcggcaa gcgcgcgcga gaagctcgcc gacatgccgt gttgacgcgc gaggcgcttg
     2401 ttcgcttcct cgcgcgaata gccgccggac agcgcgacga cacgcagcac gttcttgtgc
     2461 ttgatgaggt cggcgtagag attgtccttg ctcgggatcg tgagcttcag gatcacgcgc
     2521 tgatcgggct tcaacgcgtt cagctccttc aagagcgcgg ctttcaggag ctcctcggcc
     2581 tggtccttgt tcggcgtctt gatgtcgacc tcgggctcga cgatcggcac gaggcccgca
     2641 gcgaggattt tcttcgcgag atcgaactgc tgcttcacga ccttctcgat gccgtgcggg
     2701 tcggcctgct tgatgaccga gcgcatcttc gtgccaaaga cacctttggc cttcgccttc
     2761 ttcaacagcg cgtcgaggtt agggatcggc ttcatgagct gcacgccgtc ctgctcttcg
     2821 gcgagccctt tatcgacttt caagaacggc acgacgccct tcacctccca gaggtaggtc
     2881 ggcgtcggct tgccctcgat ctcgcggtcc atcgtgtcct cgaacaggat cgcgccgatc
     2941 agccgcttcc cgttgaaggc ggggctcgtg atgatgcgcg tgcgcatcgc gtggacgatg
     3001 tcgtacatgc ccttctcgtc cttccacgcg ctctcgtcga tgccgtagag cttcaaggct
     3061 ttcggcgtgc tgccgccgct ctggtcgagc gccgcaatga aacccttgtc gttcttgatc
     3121 ttcgcgagtt gctggtcgaa cgtgctcacg gcggactccc ttacgctctt tcggcttgtg
     3181 cgagcgtttt acgtggacgg ctattctagc gttagggcag aatccaccgc catgcgagcg
     3241 ggaggacttg cattgatcgg cgcggccgcg gcgacgctgg ccgtgcatgc gggcgacttg
     3301 ccgcccgaga tcgatcgcgt gctgacgggc cacggaattc ccgccaccga cgtcagcatc
     3361 gtcgtccagg ccgtcgacga cacgaagccc acactgagtc acctggccga cgttccgcgc
     3421 agccccgcct ccgtcatgaa gacggtcacg acctggagcg cgctcgagta cttgggcccg
     3481 gcttacacct ggccgaccga gatctacttc ctcggcgcat tcgacggccg gaagctcgac
     3541 ggcgatctcg gcatcaaggg ctacggcgac ccgcagctcg tcgtcgagga agtctggaaa
     3601 ctgctgcgtg cgctgcggcg catggggctg accgagatca gcggcgacct cgtgctcgac
     3661 gacagccatt tcgccgtcga cgagccggac cccggcgcat tcgacggcca gccgtatcgc
     3721 acctacaacg tcgtgccgag cgcgctcttg atgaacttca aggccgtgca atttcagttc
     3781 atggcggact ccgcgcgcgg gcgcgtcaac gtcgcgacgg agccggtgct ttcgaatctg
     3841 aagatcgaca acaacctcac gctcgtcgac ggcgcctgcg cgggctacaa cgcgggcatc
     3901 tctttcaacc atgccgatcc ggacgagctc gaccacgtcg ttctcgacgg caagttttcg
     3961 cgccgctgca acgtctacgg cctcggcagg acggtgctgc agcacgacac gttcgcgttc
     4021 ggcttgttct cgtcgctgtg gaaggaagtc ggcgggtcgt tctccggcaa gatgcgcaaa
     4081 gcgcagattc cgctcgacgc gacgccggcg ctcgtctggc agtcgaagcc gctcggcgag
     4141 gtcgtgcgca gcatcaacaa gaacagcaac aacgtcatga cgcggcagct gctctacacg
     4201 ctcggcgctg aagccgtcgg cgtgccggga acgcgcgaga acggcgtcgc cgtcgtgcgc
     4261 gagctgctcg cggcgcgcgg cctcgacacg agctcgctga tgctgcaaaa cggcgcgggc
     4321 ttgtcgcgcg acgagcgagc ctcgatgcag ctgctcgtcg atctgctgcg cgcggcctac
     4381 gcgagcccgt acgcgccgga gttcatcgcg tcgctgtcgc tcggcgggct cgacggcacg
     4441 acgcgcgggc ggttcggccg gttcgacgcg gcgccggcgt cgcaggtcat gcacttgaaa
     4501 acggggcggc tcgatcacgt gtcggccgtc gcgggctacg tgcgggccgc gaacggcaaa
     4561 acttatgtcg tcgccgtggt catgaactcg cccgacgcgc atcgcgggcc cgggcaggag
     4621 ctcgaagagg ccgtgttgcg gtacacgctc gcgctgcgct agcgcgcgag gtggcggcct
     4681 tttgccggcc gaaaaactac tgtaaatagc tgattattat tggctgttgg cgcttgggct
     4741 gtatataata ccagcctatt ccaacaacac ttttcggggg gcttccgatg agcgccgtga
     4801 tgtcccgttc cttggccgat tacacc
//
LOCUS       DASSNN010000253         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_425631, whole genome shotgun sequence.
ACCESSION   DASSNN010000253 DASSNN010000000
VERSION     DASSNN010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425631"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /gene="waaC"
                     /locus_tag="VFL84_04565"
     CDS             <1..811
                     /gene="waaC"
                     /locus_tag="VFL84_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HET7607928.1"
                     /translation="VIAFGLRGWRKSPLAASTWRAIRTFAADLRRTRYDAIVDLQEQV
                     KGALIARGARGTRHGFDRASIREPLAALFDNVHHRVPRDLHFVERSRRLAAAALGYRL
                     QTAPTWNLHVPATAPSLPARPYVVALHGTSRDDKLWPEDNWRSVLASFEGAGFASVLP
                     WGSPAEEARSHRLAQGLAHAVVPPWLSLPDAAALVSRAALVVGVDTGFTHLAAALRTP
                     TVALFSVTEPSRHGVECAGPHARDLGAVGAPPGVGDVLAAAGALLREAPRC"
     gene            846..2138
                     /gene="waaA"
                     /locus_tag="VFL84_04570"
     CDS             846..2138
                     /gene="waaA"
                     /locus_tag="VFL84_04570"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HET7607929.1"
                     /translation="MRRIYTTLWWLALPLLPMRLWWRGRREPGYRAHVLERFGRYATP
                     FRRTAVGALWVHAVSLGETRAAAPLIERLLREMPEQTIVLTHMTATGREAGRALFGER
                     VVQAWLPYDLPFAVRRFFAHFAPRAGLLVETELWPNVASEAVLRGVPLLLVNARLSER
                     SARGYRRVDALSRPMFAALAGVAAQSAADAARLSAAGARDVATIGNLKFDVAVTAADR
                     ERGATLRTLLGRERPVLALASTREGEETLLLDAHTRARLPSATLVLIVPRHPQRFDAV
                     AALLTERGIRFTRRSENRSVPADVRVVLGDSLGEMLAYYVAADVAFVAGSLLPLGGQN
                     LIEPIAAGTATLIGPHTFNFAQAAEAAVAAGAARRVRDADEVFTAAGELLDDAAARQR
                     MRAAGETFLAAHRGAVDRLWTWLAPRLAQADACRTADS"
BASE COUNT          262 a          792 c          804 g          334 t
ORIGIN      
        1 cgtgattgcg ttcgggctgc gcggctggcg caagtctccg ctcgcggcct cgacgtggcg
       61 cgccatccgc acgtttgcgg ccgacctgcg gcgcacgcgc tacgacgcga tcgtggacct
      121 gcaggagcag gtgaagggcg cgctgatcgc gcgcggcgcg cgcggaacgc gccacggctt
      181 cgaccgcgcg agcatccgcg agccgctcgc cgcgctcttc gacaacgttc accaccgggt
      241 gccccgcgat ctgcatttcg tcgagcgttc ccgccgcctc gcggcggcgg cgctcggcta
      301 tcggctgcag acggcgccga cgtggaacct gcacgtgccc gcgacggcgc cgtcgctgcc
      361 cgcgcggccc tacgtcgttg cgctgcacgg aaccagccgc gacgacaagc tgtggcccga
      421 ggacaactgg cgcagcgtgc tcgcgtcctt cgagggtgcc ggtttcgcga gcgtcctgcc
      481 gtggggaagc ccggccgagg aggcgcgcag ccaccggctc gcgcagggcc tcgcgcatgc
      541 ggtggtgccg ccgtggctgt cgctacccga cgccgccgcg ctcgtgtcgc gcgcggcgct
      601 cgtcgtgggc gtcgacaccg gcttcacgca tctcgccgcc gcgctgcgca cgccgacggt
      661 cgcgttgttc agcgtcaccg aaccgtcgcg ccacggcgtc gaatgcgccg gaccgcatgc
      721 gcgcgacctg ggcgcggtcg gcgcgccgcc cggcgtcggc gacgtgctcg ccgcggcggg
      781 cgcgctgctg cgcgaggcgc cgcgctgcta gcgtcccgca ccgtgctcga gaggcgctag
      841 gcgcgatgcg ccgcatctac acgacgctgt ggtggctcgc gctgccgctg ttgccgatgc
      901 gcctgtggtg gcgcgggcga cgcgagcccg gatatcgcgc gcacgtgctc gagcgcttcg
      961 ggcgttacgc gacgccgttt cgcaggaccg ccgtcggcgc gctgtgggtt catgcggtgt
     1021 cgctcggcga aacgcgcgca gcggcgccgc tgatcgagcg cctgctgcgt gagatgccgg
     1081 agcaaacgat cgtgctgacg cacatgaccg cgaccggccg cgaggcggga cgcgcgctgt
     1141 tcggcgagcg ggtcgtgcag gcgtggctgc cctacgacct gcccttcgcg gtccgccgtt
     1201 tcttcgcgca tttcgcgccg cgcgcgggtc ttctcgtcga gaccgagctg tggccgaacg
     1261 tcgcgagcga ggcggtgctg cgcggcgtgc cgctgctgct cgtcaatgcg cgcctgtcgg
     1321 aacgctcggc acgcggctat cgtcgggtcg acgcgctgtc gcggccgatg ttcgcagcgc
     1381 tcgccggcgt ggcagcgcag tcggccgccg atgccgcacg actctccgct gccggcgcac
     1441 gcgacgtcgc cacgatcggc aacctcaagt tcgacgtcgc ggtcaccgct gcggatcgcg
     1501 agcggggcgc gacgctccgc acgctcttgg gccgcgagcg gcccgtgctg gcacttgcct
     1561 cgacgcgtga gggcgaggag acgctgctcc tcgacgcgca cacccgtgcg cgactgccct
     1621 ccgcgacgct cgtgctgatc gtgccgcgcc atccgcaacg gttcgacgcg gttgcagcgc
     1681 tcctcaccga gcgaggcatc agattcacgc gccgcagcga aaatcgaagc gtgcccgccg
     1741 atgtgcgcgt cgtgctcggc gattcgctcg gggaaatgct cgcctactac gtggcagcgg
     1801 atgtcgcatt cgtggcggga agcctgctgc cgctcggtgg ccagaacctg atcgaaccga
     1861 tcgccgcagg cacagcgaca ctgatcggtc cgcacacgtt caacttcgcg caggccgcgg
     1921 aagccgcggt ggcggcaggc gcggcgcgac gcgtgcgcga tgccgacgag gtcttcaccg
     1981 ccgctggcga gcttctcgac gacgcagctg cacgccagcg gatgcgcgca gcgggggaga
     2041 ccttcctcgc cgcgcatcgc ggcgcagtcg atcgattgtg gacctggctt gccccgcggc
     2101 tcgcgcaggc cgacgcttgc cggacggctg attcgtagtt ggtagttggc agtcgtcagg
     2161 gtgcggcgcg cgctccaggt cgttgacgtc gg
//
LOCUS       DASSNN010000254         3820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1669491, whole genome shotgun sequence.
ACCESSION   DASSNN010000254 DASSNN010000000
VERSION     DASSNN010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669491"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFL84_04575"
     CDS             <1..475
                     /locus_tag="VFL84_04575"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HET7607930.1"
                     /translation="PAFVTLTGGSIGAQFGAESADVVLVFANDRAVRNIASGKFTLGG
                     DATAIAGPLGRRTQTAVTGRAEVYVYMHSRGLFAGAAFEGTRLDVDQDASADLYRNTS
                     GVPLGAQTFETPSVTLPFLDALRTAAVLPDAPSVVAPDGSRASPSGGEAVTFPLE"
     gene            complement(494..958)
                     /locus_tag="VFL84_04580"
     CDS             complement(494..958)
                     /locus_tag="VFL84_04580"
                     /inference="COORDINATES: protein motif:HMM:NF033894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HET7607931.1"
                     /translation="MGFRLRIGLTPLLAAVLVACAPKPEPRTVLDFMDDGLARDGVLA
                     RCNQNREETLHDEECMNARRAAATLALEAERARAPALERQSEAKMLALREREASASFE
                     VYADGSPSLGRPSIELGEIAPPANDLVIAEPPLEVPELSVRPRPFGDDAAQP"
     gene            complement(1124..>3820)
                     /locus_tag="VFL84_04585"
     CDS             complement(1124..>3820)
                     /locus_tag="VFL84_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HET7607932.1"
                     /translation="FEQRADALWRAALVERSRAAATLADVQAVDARLRELSEQAEAAD
                     RLLAEFWLRRATERAHAEQRDAAILLAQRAAGSDPAAAGYLAELVGDDFATLERSLRL
                     PSTPDYWHVSFADSTLVSIDGERRASRVSVAATAGASPTATAAEPLALTALAHTPLTR
                     DLAVDGDGTAGELELTVAVQHPAAGELLVTLTAPSGAAATIAVPRSEGSSVDTFTLPG
                     APGSPLARLSDEAVRGVWRLTIVDREIGNTGTFAGWGLRLGDAILARDEPNEPVPIPD
                     PARTADVAVRAAGERAVAWPVSPGAVGTVAVWNLTAGKLEHDFTLPAAPREVALDPTG
                     SRVLAATDRVLMLWNAADGALAARVGTDTDFVLPPKFSADGGYVAIAERVEGANPLYS
                     VLRSADGTLVGTIEGAPEAQAWELGPGGRYVALQGPETVIRVLETRRGAELRRLPHSH
                     AVLRLLHSADGSMLVTVDRAGSIASWPLAVASAGLGRPLGRTVAAETVSASADGRRLA
                     FTREDGAIEVLDVIAGAELYRLRLPRSVPVTRAQISDDGTRLVTQSGASLKLWQLPSK
                     PVTPRAATVDDAPTAVALDGSNDLVAVGLASGQLQIVPAAGTRSPLSFFGHRGPVTAA
                     ALNGARGLAATGGSDGIVRVWDLQSSAPTVAVAQPADAPVAIVALSSDGRYVASAAGA
                     TVRVATAADGRVAAELEAGGAVTALAFAPDSSRIAVGDATGAIVVAAIASDARARAGA
                     RLDEGVTSLAFSPDGSRLAAGDASGAIALLAAADLKREGAAHHWEQPIRWLDFSADGG
                     SLLVATDAWLHALAAAPALTPAYSKLFSWPAWSAALTAVSATSAGFAGVDASGVLTSG
                     VVDLAAPPRAATPEASALVARDWSAALALRLNDNGDPVPLEP"
BASE COUNT          561 a         1382 c         1380 g          497 t
ORIGIN      
        1 cccggcgttc gtgacgttga cgggcggaag catcggggcg caattcggcg ccgagagcgc
       61 cgacgtcgtg ctcgtgttcg cgaacgatcg cgccgtgcgc aacatcgcga gcggcaaatt
      121 cacgctgggc ggcgacgcga cggcgatcgc gggcccgctc gggcgccgca cgcagaccgc
      181 ggtgactggt cgcgccgagg tctacgtcta catgcattcg cgcggcctgt tcgccggcgc
      241 cgcgttcgag ggaacgcggc tcgacgtgga tcaggacgcg agcgccgacc tctatcgcaa
      301 cacgagcgga gtgccgctcg gcgcgcagac tttcgaaact ccgagcgtga ccctgccgtt
      361 tctcgatgcg ctgcgaaccg ccgcggtgct gccggacgcg ccgtcggtgg tcgcgccgga
      421 cggcagccgc gcctcgccga gcggcggcga agccgtgacg tttcctttgg agtgagcttc
      481 gctagagagg cggctagggc tgcgcggcgt cgtcgccgaa cggccgcggt ctgaccgaga
      541 gctcgggcac ctcgagcggc ggctcggcga tcacgagatc gttcgccggc ggcgcgatct
      601 cgcctagctc gatgctgggc cggccgagcg acgggctgcc gtcggcatag acctcgaacg
      661 acgccgaggc ctcgcgctcg cgcaatgcca gcatcttcgc ttcggactgc cgctcgagcg
      721 cgggcgcgcg agcgcgctcg gcctcgagcg ccagcgtcgc tgccgcgcgg cgcgcgttca
      781 tgcactcctc gtcgtgcagc gtctcttcgc gattctgatt acagcgggca agaacgccat
      841 ccctggcgag gccgtcgtcc atgaaatcga gaaccgtacg cggctcgggc ttcggcgcgc
      901 aggcgacgag caccgctgcg agcagcggcg tcaagcctat acggaggcga aaccccaact
      961 cccgatgtct tcgaattgcg accgatgcga acacgttgaa gctcgatgaa atgacggtta
     1021 cgtaaaacgt agcgaaccat agcgcgtgat cgcgaagagc gcccaccgtg cagttcacag
     1081 attcgacgaa tttgtcgctt gcgtttgcgc gacgcggctc gaatcacggc tcgagcggca
     1141 ccggatcccc attatcgtta agacgcagcg cgagggccgc ggaccaatcg cgcgcgacga
     1201 gcgcgctcgc ttccggcgta gcggcacgcg gcggcgcagc gaggtcgact acgccggacg
     1261 tcaggacgcc gctcgcatcg actccggcga agcccgcgga cgtcgcggat accgcggtca
     1321 gcgccgcgct ccacgcgggc cacgagaaga gcttgctgta cgccggcgtg agcgcgggag
     1381 ccgcggcgag cgcgtgcagc cacgcgtcgg tcgcgacgag caacgagccg ccgtcggcgc
     1441 tgaagtcgag ccaacggatc ggctgctccc aatggtgcgc tgcgccttcg cgcttcaagt
     1501 ccgcggccgc gagcagcgcg atcgcgccgc tcgcgtcgcc tgcggcgagc cggctgccgt
     1561 cgggcgagaa cgccaacgac gtgacgccct catcgagccg agcgccggcg cgcgcgcgag
     1621 catcggacgc gatcgcggca acgacgatcg cgcccgtcgc gtcgccgacc gcgatccgcg
     1681 aggaatccgg cgcgaaggcc aatgccgtca ccgcgccgcc cgcttcgagc tccgcggcca
     1741 cacgcccgtc cgcggccgtc gcgacgcgca ccgtggcgcc ggcggcgctc gcgacgtaac
     1801 gcccgtccga gctcagcgcg acgatcgcga cgggcgcatc ggccggctgc gcgacggcga
     1861 ccgtgggcgc gctcgactgc aagtcccaca cgcgcacgat gccgtcgctg ccgccggtcg
     1921 ccgcgaggcc gcgcgcgccg ttcaacgccg cggccgtgac tgggccgcga tgcccgaaaa
     1981 acgacagcgg ggagcgcgtt cctgccgccg gcacgatctg cagctggccg ctcgccaaac
     2041 cgacggcgac gaggtcgttg cttccgtcga gcgcgaccgc ggtcggcgcg tcgtcgacgg
     2101 tcgcggcacg cggcgtgacc ggtttgctcg gcaattgcca gagcttcagc gaagctccgc
     2161 tctgagtgac gagccgcgtg ccgtcgtccg agatctgagc tcgagtgacg ggcacggagc
     2221 gcggcaggcg cagacgatac agctcggctc ccgcgatgac gtcgagcact tcgatggcgc
     2281 cgtcctcgcg cgtgaacgcg agccttcggc cgtcggccga agcgctgacg gtctccgcgg
     2341 cgaccgtgcg gccgagcggc cggccgaggc ccgcactcgc cacggcaagc ggccacgagg
     2401 cgatcgagcc ggcgcggtcc acggtcacga gcatcgatcc gtcggcggaa tgcagcagcc
     2461 gcaggacggc atgcgaatgc ggcagacgcc tgagctccgc gccgcgccgc gtctcgagca
     2521 cgcggatgac cgtctcgggt ccttgcagcg cgacgtaacg cccgcccggg ccgagctccc
     2581 acgcttgcgc ttccggcgcg ccttcgatcg tgccgacgag cgtgccgtcg gcgctgcgca
     2641 gcacgctgta gagcggattc gcgccttcga cgcgctcggc gatcgcgaca tagccgccgt
     2701 cggccgagaa cttgggcggc agaacgaaat cggtgtccgt gccgacacgc gccgcgaggg
     2761 ctccgtcggc ggcattccag agcatcagca cgcgatccgt cgccgcgagc acgcgcgagc
     2821 cggtcgggtc gagcgcaacc tcgcgcggcg cggccggcaa cgtgaagtcg tgctcgagct
     2881 tgcccgccgt cagattccag accgccaccg tgccgaccgc gcccggcgag acgggccacg
     2941 caacggcgcg ctcgccggcg gcccgcacgg cgacgtcggc ggtgcgcgcg gggtcgggga
     3001 ttggcacggg ctcgttgggc tcgtcgcggg cgaggatcgc gtcgccgagc cgcaaacccc
     3061 aaccggcgaa ggtgccggtg ttgccgattt cgcgatcgac gatcgtgagc cgccacacgc
     3121 cgcgcacggc ttcgtcggag agccgcgcga gcggcgagcc gggcgcgcct ggcagcgtga
     3181 atgtatcgac ggagctgccc tcgctgcgcg ggacggcgat cgtcgccgcg gcgccgctcg
     3241 gcgccgtcaa cgtcacgagc agctcgccgg ccgcgggatg ctgcacggcg acggtgagct
     3301 cgagctcgcc ggccgttccg tctccatcga cggcgagatc gcgggtcaac ggcgtgtgcg
     3361 cgagcgccgt gagcgcgagc ggctccgctg cggtcgcggt tgggctcgcc cccgccgtcg
     3421 ccgcgacgga cacacgcgac gctcgccgct ctccgtcgat cgagacgagc gtcgaatccg
     3481 cgaacgacac gtgccaatag tccggcgtgc tcggcaggcg cagcgaccgc tcgagcgtcg
     3541 caaagtcgtc gccgacgagc tcggcgagat agccggcggc agccggatcg gagccggccg
     3601 cgcgttgcgc gagcaggatc gccgcgtcgc gctgctccgc atgcgcgcgc tccgtggcgc
     3661 gccgcagcca gaactcggcg agcaaacgat ccgccgcttc ggcttgctcc gacagctcgc
     3721 gcagccgcgc atcgacggcc tgaacgtccg cgagcgtcgc cgcggcgcgg ctcctctcga
     3781 cgagcgcggc gcgccagagc gcgtcggcgc gctgctcgaa
//
LOCUS       DASSNN010000255         4227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_658715, whole genome shotgun sequence.
ACCESSION   DASSNN010000255 DASSNN010000000
VERSION     DASSNN010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658715"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VFL84_04590"
     CDS             complement(<1..607)
                     /locus_tag="VFL84_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HET7607933.1"
                     /translation="MTPTLLARHAARALLIAASLAFFLDASSAFAFDFDDVAAIAQRK
                     ARAAYQPPDRRQPAELELLGYDQYRDIRFRPDRAIWRAEGAPFDLMFFHRAQSNPRVR
                     MNEIVDGEPRHIPYDSADFDFGKNTLSPEKWGELDYAGLRVHYHLNGPDYKDELIVFL
                     GASYFRALAAGTRYGLSARGLAVDTVGAAGEEFPYFPEFWIV"
     gene            695..1654
                     /gene="ccmA"
                     /locus_tag="VFL84_04595"
     CDS             695..1654
                     /gene="ccmA"
                     /locus_tag="VFL84_04595"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HET7607934.1"
                     /translation="MSAAAAISVDRLVVNRGERRVLDEITFDVASGEIVGLIGPNGAG
                     KTTTLAVLSTLLAPDAGEIRVAGHDLRRAPHAVRRSIGRVPQETALYPTLTGRENAAF
                     FARLVGASAADARHAAAAALEQVGLAARADERTGVYSGGMQRRLSLACGLLGSPPVLL
                     LDEPTVGVDPQSFESIVAAIRAHARGGAALLLSTHHMEEAEHLCDRVVLIDRGRVVAT
                     GPPAHLIREAVPGLLIDVVTDEALPEPWLDGIAGARACAAQDTGSSGMARGRAARVAL
                     ADVELAPRVLERAARERMVLDFRVHRPSLHDAFLELTGAAPRD"
     gene            1667..3361
                     /locus_tag="VFL84_04600"
     CDS             1667..3361
                     /locus_tag="VFL84_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607935.1"
                     /translation="MMGLLGVIIAKDLRLLLRDRAALVFLTIAPIVVMSVAGFSLAPL
                     YGADAGGRVTYELPIVDEDGGDLATQIENALRGDETLSVRRVSSRAEAQQLVRDKAAG
                     SALVIPTGTRDALAAGRPARLLLYTDPVRFLDRINMTLRVAKARDALADAERARIVKE
                     LADQRAQARAQLEGLDASLQRVRANLEAMRREAGAERARAAGALDTELDRLQAEVSGE
                     LDARIKDLAAQVNAAVAERVGALQEPARAYLDELMAARRELEAWFAELQRLAARRADR
                     IPDPPAFPEPPPELTRAIEEPPAPIELPVNVELRILPRLRAALDALDGLAVGEVDVQV
                     PDLELPEAVIPAASLGVEEISIDGGPTEINPFDQNVPGFSVTFMLLGMLFAVALGLFD
                     ERDLRTFDRVRALPVPVRNIVLGKLASRFAVGVVQMLVLFAVGFALFRVSLGPQPWAL
                     LLPILGIVFVGTAIGLVAAAVAPSRDAVVPLGAIVILTMAAVGGCWWPIDLEPEWMHR
                     VALAFPTTWAMDAFNDLMLRGRRVEAVYPSIAALFAHGAAYLTVGLVLFRLRYSKA"
     gene            complement(3365..>4227)
                     /locus_tag="VFL84_04605"
     CDS             complement(3365..>4227)
                     /locus_tag="VFL84_04605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HET7607936.1"
                     /translation="SDRIKPYTVLMAMNNAAASWIGIEQRLSGPNLTFSTACSSSAVA
                     IGEAWLRVRSGATPVMLAGGAEAPLTLGTLKAWEALRTLASEDPADPSASCKPFAKDR
                     SGLVLGEGAAVFVLEEWEHAARRGATVQGEIVGYGLSTDGAHITRPTVDGQARAMQLA
                     LDSAGVGADAIAYINAHGTATLQNDAVETAAIRRVFASHADRVAVSSSKSMHGHLLGA
                     AGAVELVATLLAVRTGTIPPTLNLHIPDPECDLDYVPNVARRDQDIELAMTNSFAFGG
                     TNAVLVCRRA"
BASE COUNT          584 a         1462 c         1500 g          681 t
ORIGIN      
        1 tcacgatcca gaattcgggg aagtagggga actcctcgcc ggccgcgccc acggtgtcga
       61 cggccaagcc gcgtgccgac aacccgtaac gcgtgcccgc cgccagcgcg cggaagtagc
      121 tcgcgccaag gaacacgatg agctcgtctt tgtagtcggg cccgttcagg tggtagtgca
      181 cgcgcagccc ggcgtagtcg agctcgcccc acttctcggg cgaaagcgtg ttcttgccga
      241 agtcgaagtc ggcgctgtcg tagggaatgt gccgcggctc gccgtcgacg atctcgttca
      301 tgcgcacgcg cgggttcgat tgcgcgcgat gaaagaacat caagtcgaac ggcgcgccct
      361 cggctcgcca gatcgcgcgg tcggggcgaa aacgtatgtc gcgatactga tcgtagccga
      421 gcagctcgag ctcggcgggc tgcctgcggt cgggcggctg gtaggcggcg cgcgctttgc
      481 gctgcgcgat cgccgcgacg tcgtcgaagt cgaacgcgaa cgccgagctc gcgtcgagaa
      541 aaaacgcgag cgacgcggcg atgagcagcg cgcgcgcggc gtgtcgtgca agaagcgtcg
      601 gggtcatgat ccaatccgta agtacctcca tgcaacatag cccgttgccc ccgccttacg
      661 ctagggtcca cggcagcggt gctatgctcg ggccgtgtcc gccgctgccg ccatttccgt
      721 cgatcgactc gtcgtgaatc gcggcgagcg gcgcgtcctc gacgagatta cgttcgatgt
      781 tgcgtccggc gagatcgtag gtctgatcgg cccgaacgga gccggcaaga cgacgacgct
      841 cgcggtgctc tcgacgctgc tcgcccccga cgcgggggag attcgcgtcg cggggcacga
      901 tctgcgccgc gcgccgcacg cggtgcgccg ctcgatcggc cgtgtgccgc aggaaacggc
      961 tctgtacccc acgctgaccg gccgcgagaa tgccgcgttc ttcgcgcgcc tcgtcggcgc
     1021 gagtgccgcc gacgcgcgcc acgcggccgc cgcggcgctc gagcaagtcg ggctcgccgc
     1081 gcgcgcggac gagcgcaccg gcgtctattc cggcgggatg cagcggcggc tgagcctcgc
     1141 ctgcgggctg ctcggctcgc cgcccgtgct gctgctcgac gagccgaccg tcggcgtcga
     1201 tccgcagtcg ttcgagagca tcgtcgcggc gatccgcgcg cacgcccggg gcggtgcggc
     1261 gctgctcttg agcacccatc acatggagga agcggagcat ctctgcgatc gcgtcgtctt
     1321 gatcgatcgc ggccgcgtcg ttgcgaccgg tccgcccgcg catctgatcc gcgaagccgt
     1381 acccggattg ctgatcgacg tcgtgacgga tgaagcgctg cccgagccgt ggctcgacgg
     1441 catcgccggc gctcgtgcgt gtgcggcgca ggatacgggc agcagcggga tggcgcgcgg
     1501 acgggccgca cgagtcgccc tcgcggacgt cgagctcgcg ccgcgcgtgc tcgagcgtgc
     1561 ggcgcgcgag cgcatggtgc tcgatttccg cgtgcaccgt ccgagcttgc acgacgcgtt
     1621 tctcgagctg accggcgccg cgccgcgtga ttgagggcag ccggcaatga tgggcttgct
     1681 cggcgtgatc atcgcgaagg atctgcgttt gctgctgcgc gatcgcgcgg ctctcgtgtt
     1741 cctcacgatc gcgccgatcg tcgtgatgtc ggtcgcggga ttctcgctcg cgcctcttta
     1801 cggcgccgac gccggcgggc gcgtgaccta tgagttgccg atcgtcgacg aggacggcgg
     1861 cgacttggcg acgcaaatcg agaacgcatt gcgcggcgac gaaaccttga gcgtgcgccg
     1921 cgtctcctca cgcgccgagg cgcagcagct cgttcgcgac aaggccgccg gcagcgccct
     1981 cgtgataccc acgggcactc gcgatgcgct cgcggccggc cggccggcgc gcctgctctt
     2041 gtacacggac ccggtcaggt tcctcgaccg catcaacatg acactgcgcg tcgcgaaagc
     2101 gcgcgacgcg ctggccgacg ccgagcgcgc gcgcatcgtc aaggagctcg ccgaccagcg
     2161 cgcgcaggcg agggcacagc tcgaggggct cgatgcgagc ctgcagcggg tgcgcgccaa
     2221 tctcgaagcg atgcggcgag aggcgggcgc ggagcgcgcg cgagccgccg gcgcgctcga
     2281 cacggagctc gaccgtcttc aagcggaagt gtccggcgag ctcgacgcgc gcatcaagga
     2341 tctcgctgcg caggtgaacg cggcggtcgc cgagcgcgtg ggcgcgctgc aagagcccgc
     2401 gcgcgcgtac ctcgacgagc tgatggcggc acgccgcgag ctcgaggcgt ggttcgcgga
     2461 gctgcagcgg ctcgccgctc gacgtgcgga ccggattccg gatccgcccg cgtttccgga
     2521 gccgccgccc gagcttacgc gcgcgatcga ggagccgccc gcgccgatcg agttgcccgt
     2581 caacgtcgag ctgcgcattc tgccgcgcct gcgcgcggcg ctcgacgcgc tcgacgggct
     2641 cgccgtcggc gaggtcgacg ttcaggttcc cgatctcgag ttgccggaag ccgtgattcc
     2701 ggccgcgtcg ctcggcgtcg aggagatcag catcgacggc ggcccaaccg agatcaaccc
     2761 gttcgaccag aacgttccgg gcttcagcgt cacgttcatg ctgctcggca tgctgttcgc
     2821 cgtggcgctc gggctgttcg acgagcgcga cttgaggacg ttcgatcgcg tgcgggctct
     2881 gcccgtgccg gtgcgcaaca tcgtgctcgg caagctcgcg tcgcgattcg ccgtcggcgt
     2941 cgtgcaaatg ctcgtgttgt tcgcggttgg cttcgcgctg tttcgggtct cgctggggcc
     3001 gcagccgtgg gcgttgctct tgccgatcct cggcatcgtg ttcgtcggca ccgcgatcgg
     3061 cctcgtcgcc gccgcggtcg ccccgagccg cgacgcggtc gtgcctctcg gcgccatcgt
     3121 gatcctcacg atggccgcgg tcggcggctg ctggtggccg atagacctcg agcccgaatg
     3181 gatgcatcgc gtcgcgctcg cgttcccgac gacttgggct atggatgcgt tcaacgatct
     3241 gatgctgcgc ggccgccgcg tggaggcggt ttacccctcc atcgccgcgc tgttcgcgca
     3301 cggcgctgcg tatctcacgg tcgggctcgt gctgttccgg ctccgttact ccaaagcgta
     3361 gcgctcacgc acgccggcaa acgagcaccg cgttggtgcc gccgaacgcg aaggagttcg
     3421 tcatcgcgag ctcgatgtct tgatcgcgcc gtgcgacgtt cggcacataa tcgaggtcgc
     3481 attcgggatc gggtatgtgc aggttcagcg tcggcgggat cgtgcccgtg cggacggcga
     3541 gcagcgtcgc gacgagctcg acggcgccgg ccgcgccgag caggtggccg tgcatcgact
     3601 tgctcgagct gacggccacg cggtcggcgt gcgacgcgaa gacgcgccga attgcggccg
     3661 tttcgacggc atcgttctgc agcgtcgcgg tgccgtgcgc gttgatgtac gcgatcgcgt
     3721 ccgcgccgac gcccgcggag tcgagcgcga gctgcatcgc gcgcgcctga ccgtcgacgg
     3781 tcggccgcgt gatgtgagcg ccgtccgtgg agagcccgta gccgacgatc tcgccttgca
     3841 ccgtcgcgcc gcgccgcgcc gcgtgctccc actcctcgag cacgaacacg gccgcgcctt
     3901 cgcctaaaac gaggccgctg cggtccttcg caaacggctt gcacgacgcc gacggatcgg
     3961 cagggtcttc gctcgcgagc gtgcgaagcg cctcccaagc tttgagcgtg ccgagcgtca
     4021 acggcgcttc ggcgccgccc gcgagcatga ccggcgtcgc gccgctgcgg acgcgcagcc
     4081 acgcctcgcc gatcgcgacg gccgacgacg agcacgccgt cgagaacgtc aggttcgggc
     4141 ccgacagccg ctgctcgatc ccgatccaag acgccgcggc gttgttcatc gccatcagca
     4201 cggtgtacgg cttgatgcga tccgagc
//
LOCUS       DASSNN010000256         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1179533, whole genome shotgun sequence.
ACCESSION   DASSNN010000256 DASSNN010000000
VERSION     DASSNN010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179533"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFL84_04610"
     CDS             <1..243
                     /locus_tag="VFL84_04610"
                     /inference="COORDINATES: protein motif:HMM:NF027718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-like domain-containing protein"
                     /protein_id="HET7607937.1"
                     /translation="TSRIVVTNPVDTTLLGTYTVTYNVSDLSGNAAKPVTRTVNVQPQ
                     AAVLEGSGGGAVGFEMLIALLLVAAFAMPRRDRYSR"
     gene            complement(235..1125)
                     /locus_tag="VFL84_04615"
     CDS             complement(235..1125)
                     /locus_tag="VFL84_04615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HET7607938.1"
                     /translation="MPQHDGQTGHNRFGDKSLLITALRLAAKSLPGDYVRTFVYLNLI
                     YKPRLALRRLINTFYRMDHVYAVIEEFKRGYKGPFSILEFGVADGYAFAKKAFATRYL
                     GMEDGIVCHGFDSFEGLPDTDDARDLASVAGADWKAGQYRGRYEELRDYLDARGYRNC
                     RLHRGLFEATLTPEFLATLRVHKPILVWIDCDYYTSARSVLERLIPYLPSGCVVYFDE
                     YEFNFGSRFTGESRLIHEINRGDFGDGIELVLDTDLSWDLRRCYRFINRHAHCVLERK
                     RELRRLVEWRKRAGNDSPLP"
     gene            complement(1178..>2745)
                     /locus_tag="VFL84_04620"
     CDS             complement(1178..>2745)
                     /locus_tag="VFL84_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607939.1"
                     /translation="PPAATELHPGLGDYHFPITTAMPEAQVYFDHGIRLLYGFNHDEA
                     ARYFRRAAELDPQAPMPYWGVALSIGPNYNDTAVDAARAQATYEAVQSARQRAANASE
                     RERDYVAAIAKRYPSPEPASDWLAFHRDYSEAMRAMVQKYPDDVDAATLFAESLMMLR
                     PWQLWSLDGEPAPGTLELVAVLESVLKREPEHPGANHFYIHAVEASRNLERAIPSAMR
                     LMTLTPGAGHLVHMPGHIFLQTGDFDLAAETNVEASDADRAFIERTGATGVYPLMYFT
                     HNIHFIAYARAQQGRYDDAKRAALDMVSNVGDADLTMQMLEGFRLYPAMIDLRFHRWE
                     ALLAAPEPETTRALSHAFWRYARAMALAGQGRARDASAEQRRFERERAALAPDIQYLA
                     NNKAADLLALSAATLDAALAEARGDSAVAIEAWRRAVTLEATLQYDEPPAWFYAVRQS
                     LGAALLRGGQAAEAEHVFREALAAHPRDGRLLYGLWQSLLAQNRGNEAVLVQGQFTRA
                     WAGATVPLTLKDL"
BASE COUNT          439 a          909 c          942 g          455 t
ORIGIN      
        1 acgtcgcgga tcgtcgtcac gaatcccgtc gacacgacgt tgctcggcac ctacaccgtc
       61 acgtacaacg tcagcgactt gtcgggcaac gcagcgaagc ccgtcacgcg gaccgtcaac
      121 gtgcagccgc aagccgccgt gctcgagggc agcggcggcg gcgcggtcgg cttcgagatg
      181 ctgatcgcgt tgctgctggt cgccgcgttt gcgatgccgc ggcgcgatcg gtattcaagg
      241 taacggggag tcgtttccgg cgcgcttgcg ccattccacg aggcggcgca gctcgcgctt
      301 gcgctcgagc acgcaatgcg cgtgccggtt gatgaagcga tagcagcggc gcaggtccca
      361 ggagagatcc gtatcgagca cgagctcgat gccgtcgccg aaatcgccgc gattgatctc
      421 gtggatcagc cgcgactcgc ccgtgaagcg gctgccgaaa ttgaactcgt actcgtcgaa
      481 gtagacgacg cacccgctcg gcaagtatgg gatcaaccgc tcgagcaccg agcgcgcgga
      541 cgtgtagtag tcgcagtcga tccaaacgag aatgggcttg tgcacgcgca gagtcgccag
      601 gaattccggc gtcaacgtcg cttcgaacag gccgcggtgc agccggcaat ttcggtaacc
      661 gcgcgcgtcg agatagtcgc gcagctcctc gtagcgcccg cggtattgcc cggccttcca
      721 gtccgcgccg gcgacgcttg ccaagtcgcg agcgtcgtcg gtgtcgggca gcccctcgaa
      781 gctgtcgaag ccgtggcaga cgatgccgtc ttccatgccg agatagcgcg tcgcaaaggc
      841 ctttttcgcg aacgcgtagc cgtcggcgac gccgaactcg aggatcgaga acggaccctt
      901 gtagccgcgc ttgaactctt cgatgaccgc gtagacgtgg tccatgcggt agaatgtgtt
      961 gatgagccgg cgcaacgcga gacgcggctt gtagatcagg ttcaggtaga cgaacgtgcg
     1021 cacgtagtcg cccggcagcg acttggcggc gagccgcagc gccgtgatca agagcgattt
     1081 gtccccgaac cggttgtgtc ccgtctgacc gtcgtgttgt ggcataggct tttagcccat
     1141 gccttgcaat ccccgggcca acgaggcgcg caaaagatta cagatcctta agcgtcagcg
     1201 gcaccgtcgc gccggcccac gcgcgcgtga actgcccctg cacgagcacg gcctcgttgc
     1261 cgcggttctg cgcgagcaag ctctgccaga ggccatagag caagcgcccg tcgcgcgggt
     1321 gcgccgcgag cgcctcgcgg aacacgtgct ccgcctccgc cgcttggcct ccgcgcagca
     1381 acgcggcgcc gaggctctgg cgcacggcgt agaaccaggc gggcggctcg tcgtattgaa
     1441 gcgtcgcttc tagcgtcacg gcgcggcgcc aagcctcgat cgcgaccgcg ctgtcgccgc
     1501 gcgcctccgc gagcgcggcg tcgagcgtgg cagcgctcag tgcgagaaga tcggccgcct
     1561 tgttgttggc caggtactgg atatcgggcg caagcgccgc gcgctcgcgc tcgaagcggc
     1621 gctgctcggc agacgcgtcg cgcgccctgc cctggcccgc cagcgccatc gcgcgcgcgt
     1681 agcgccagaa cgcgtggctc aatgcgcgcg tcgtctcggg ctccggcgcc gcgagcaacg
     1741 cttcccagcg gtggaagcgc agatcgatca tcgccggata gaggcggaac ccttcgagca
     1801 tctgcatcgt aaggtctgcg tcgccgacgt tgctgaccat gtcgagcgcc gcccgcttcg
     1861 cgtcgtcgta gcgcccctgc tgcgcgcgcg cgtaggcgat gaagtggatg ttgtgcgtga
     1921 agtacatgag cgggtacacg cccgtcgcgc cggtgcgctc gatgaacgcg cgatccgcgt
     1981 ccgacgcctc gacgttcgtt tccgcggcga gatcgaagtc gcccgtttgc agaaagatgt
     2041 ggcccggcat gtgcacgaga tggccggctc ccggcgtcag cgtcatgagg cgcatcgcgc
     2101 tcgggatcgc gcgctcgagg ttgcgcgacg cctcgacggc gtgaatgtag aaatgattcg
     2161 cgccgggatg ctcgggctcg cgcttcagca ccgactcgag caccgcgacg agctcgagcg
     2221 tgccgggcgc cggctcaccg tcgaggctcc agagctgcca cgggcgcagc atcatgaggc
     2281 tctcggcgaa cagcgtcgcg gcgtcgacgt cgtcgggata cttctgcacc atcgctcgca
     2341 tcgcttcgct gtagtcgcga tggaacgcga gccagtccga tgccggctcc ggcgacggat
     2401 agcgcttcgc gatggccgcg acgtagtcgc gctcgcgctc gctcgcgttt gcggcgcgct
     2461 gccgcgcgct ctggacggcc tcgtacgtcg cctgcgcgcg cgcggcgtcg acggccgtgt
     2521 cgttgtaatt cggcccgatc gacagcgcga cgccccagta cggcatcggc gcttgcgggt
     2581 cgagctcggc cgcgcggcgg aagtagcggg ccgcttcgtc gtggttgaag ccgtagagca
     2641 gacgaatgcc gtgatcgaaa tagacctgcg cctcgggcat agccgtcgtg atcgggaagt
     2701 gatagtcgcc gagccccgga tgaagctcgg tcgccgccgg cggca
//
LOCUS       DASSNN010000257         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_178115, whole genome shotgun sequence.
ACCESSION   DASSNN010000257 DASSNN010000000
VERSION     DASSNN010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178115"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..524
                     /locus_tag="VFL84_04625"
     CDS             114..524
                     /locus_tag="VFL84_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019676200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7607940.1"
                     /translation="MFILKPHSGVPIYRQLSEQIRRMAASGQLPPGTELPSIRDLALK
                     HAINPMTVSKVYTLLEAEGVLERNRGKPMTVAKPKRTESPVAKRLEQLEPHVEQVALA
                     ARQLELSEKDVVKALHETWEKTDVESLHRRARPR"
     gene            487..1365
                     /locus_tag="VFL84_04630"
     CDS             487..1365
                     /locus_tag="VFL84_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008950586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7607941.1"
                     /translation="MSSPYIAARGLGKSFNGKPVLADVSFTVEPGDVVGVLGKNGAGK
                     TTLLELVLGFTPATAGGVQLFGYDSYRLPGAAKARIGFVPQQDELVNQLTAADQIGVI
                     ASFYPKWDDELVGRLSREWEVDLNQRVKNMSVGQRQKLSILLGLGHKPDVLILDEPVA
                     SLDPVARRQFLEQIIEVAADGNRSVVFSSHIVSDVERLANKIWILKDQRMYWQGDFDS
                     LKDSIVRVHARATRPLPVSFAIPNALSVQRNGTTATAIVQGWRAEMHDGLARQLEAEI
                     EVEQLTLEDIFLELHR"
     gene            1362..>2116
                     /locus_tag="VFL84_04635"
     CDS             1362..>2116
                     /locus_tag="VFL84_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607942.1"
                     /translation="MKAIARLVYTSFTGTALLRFITLAGLVAGIGGAALNMYLPPLTV
                     RASGGPSRFSLALETLIFLLPVAGLLSFVFGASLLPALFARLATSHYLYVLPHGRTKL
                     LAGAFATVALTACFAAGIIVMYYYKTPLALDLIFQRAVSVSFVTVSFLYVVLWLTGKS
                     GTIGLLVGSFVMVATLVLPLRFIATPSTSLALPWTACAVLWGVFATGFLLAPRLKAPL
                     GRLRHALAQLTGSSYRGGGEIDFLIGTARPWTLA"
BASE COUNT          323 a          719 c          686 g          388 t
ORIGIN      
        1 gagtggagct ggccgcgcca agcgtgattc gcgtcgcagt ttcgcgcggc tgagttgcct
       61 ctcttccggc ctatgtgcat tattgcacta atgcacttgg cggatagcga cgcttgttca
      121 ttctgaagcc gcattcgggc gtgccgatct atcggcagct ctcggagcag atccggcgca
      181 tggccgcgag cggccagctg ccgcccggca ccgagctgcc gtcgatccgc gatctcgcgc
      241 tgaagcacgc gatcaacccg atgacggtgt cgaaggttta cacgctgctc gaggcggaag
      301 gcgtgctcga gcgcaaccgc ggcaaaccga tgaccgtcgc taaaccgaag cgcacggagt
      361 ccccggtcgc gaagcgcctc gagcagctcg agccgcacgt cgagcaggtc gcgctcgccg
      421 ctcgccagct cgagctcagc gaaaaagacg tcgtcaaagc cttgcacgag acttgggaga
      481 aaaccgatgt cgagtcccta catcgccgcg cgcggcctcg gtaagtcgtt caatggcaaa
      541 ccggtgctgg ccgacgtgtc gttcaccgtc gagcccggcg acgtcgtcgg cgtgctcggc
      601 aagaacggcg ccggcaaaac gacgctgctc gagctcgtgc tcggcttcac accggcgacg
      661 gccggcggcg tgcagctgtt cggctacgac agctatcgcc tgcccggcgc ggcgaaggcg
      721 cgcatcggct tcgtgccgca gcaggacgag ctcgtcaatc agctgacggc cgccgatcag
      781 atcggcgtca tcgcctcgtt ctatccgaag tgggacgacg agctcgtcgg ccggttgagc
      841 cgggagtggg aagtggatct aaaccagcgc gtcaagaaca tgtcggtcgg gcagcggcag
      901 aagctgtcga tcctgctcgg cctcggccac aaacccgacg tgctgatcct cgacgagccc
      961 gtcgcgagcc tcgatccggt ggcgcgccgc cagttcctcg agcagatcat cgaggtcgcg
     1021 gccgacggca accggtccgt cgtgttctcg tcgcacatcg tctcggacgt cgagcggctc
     1081 gcgaacaaga tctggatcct caaggaccag cgcatgtatt ggcagggcga cttcgactcg
     1141 ctgaaggact cgatcgtgcg cgtgcacgcg cgcgcgacgc ggccgctgcc cgtctcgttc
     1201 gcgattccga atgcgctcag cgtgcagcgc aacggcacga cggcgaccgc gatcgtgcaa
     1261 ggctggcgcg cagagatgca cgacggcctc gcgcggcagc tcgaggccga gatcgaggtc
     1321 gagcagctca cgctcgagga catcttcctg gagctgcatc gatgaaggcg atcgcgcgct
     1381 tggtctacac gagcttcacg ggcaccgcgc tgctgcgctt catcacactc gccggcctgg
     1441 tcgccggtat cggcggcgcg gcgctgaata tgtacctgcc gccgttgacg gttcgcgcga
     1501 gcggcggtcc atcgcgcttc tcgcttgcgc tagagacttt gatctttctg ctgcccgtcg
     1561 ccgggctcct gagcttcgtg ttcggcgcgt cgctgctgcc ggcgctgttc gcgcgcctcg
     1621 ccacgagcca ctatctctac gtgctcccac acggccgcac gaagctgctc gcgggcgcct
     1681 tcgcgaccgt cgcgctgacc gcgtgcttcg cggccggcat catagtcatg tactactaca
     1741 agacgccgct cgcgctcgat ctcatctttc aacgcgcggt ctcggtctcg ttcgtgacgg
     1801 tcagctttct ctacgtcgtg ctgtggctga cggggaagag cggcacgatc gggctgctcg
     1861 tcggctcctt cgtgatggtc gcgacgctcg tgttgccgtt gcgcttcata gcgacgccct
     1921 ccacctcgct cgccttgcca tggaccgcgt gtgcggtgct gtggggcgtc tttgccacgg
     1981 gcttcctcct ggcgccgcgc ctcaaagcgc cactcggccg cttgcggcac gcgctcgccc
     2041 agctcacggg ctcttcttac cgcggcggcg gcgagatcga tttcctgatc ggcacggcgc
     2101 gcccgtggac tctcgc
//
LOCUS       DASSNN010000258         3802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1059348, whole genome shotgun sequence.
ACCESSION   DASSNN010000258 DASSNN010000000
VERSION     DASSNN010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059348"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFL84_04640"
     CDS             complement(<1..348)
                     /locus_tag="VFL84_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607943.1"
                     /translation="MNFITALQADRLIAQIREESDPSSATGKQLFAKLGKLGTAAIPK
                     ILEALASADKRQTTEYIDILGSLINDKTLSLVTRGLADPEPRTVSATATALAANKRFN
                     VNRLVDLLGEDEYS"
     gene            591..1685
                     /gene="apbC"
                     /locus_tag="VFL84_04645"
     CDS             591..1685
                     /gene="apbC"
                     /locus_tag="VFL84_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HET7607944.1"
                     /translation="MSDSARSTLQEALGSFVVPYTGRPLGGPGAAFTLERRGSVWHVT
                     VQLGFPLAASGEALADAVRMHCAAALEGASLEITIESSIVAHAVQHGLKPLPGAHNLI
                     AVASGKGGVGKSTVAVNFALALKAEGAKVGLLDADIYGPSQPRMLGLVGRRPETRDGK
                     LLEPLVAHGIEAMSIGFLVDEQQPMAWRGPMVTSALNQLLTQTRWGDLDYLIVDMPPG
                     TGDIQLTLAQRVPVSGAVIVTTPQDIALADARKGLEMFQKVHVPVLGVVENMSLHICS
                     RCGHEEHIFGEHGGRNLAEQYGVPLLGALPLDRRIREETDQGKPTVAADPKSPLARAF
                     TEAALRAAGELAARAKDYSRLFPNITVEDN"
     gene            1685..2017
                     /gene="dcd"
                     /locus_tag="VFL84_04650"
                     /pseudo
     CDS             1685..2017
                     /gene="dcd"
                     /locus_tag="VFL84_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632155.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
     gene            1994..3246
                     /locus_tag="VFL84_04655"
                     /pseudo
     CDS             1994..3246
                     /locus_tag="VFL84_04655"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
     gene            3251..3484
                     /gene="dcd"
                     /locus_tag="VFL84_04660"
                     /pseudo
     CDS             3251..3484
                     /gene="dcd"
                     /locus_tag="VFL84_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432068.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
     gene            complement(3495..>3802)
                     /locus_tag="VFL84_04665"
     CDS             complement(3495..>3802)
                     /locus_tag="VFL84_04665"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HET7607945.1"
                     /translation="VKAAVMRDMAFKGAPGPYQIADEDSVGTYEYTDSGPATLQTGVG
                     SLETQLFVQHREGSSRSTWLWVAPKLSFLPVRIEQRRNDEVQTSFLLQTVDGLAPSR"
BASE COUNT          676 a         1205 c         1232 g          689 t
ORIGIN      
        1 cgagtactcg tcctcgccga gcaggtcgac gagccggttg acgttgaacc gcttgttggc
       61 cgccaacgcc gtcgcagtgg ccgagaccgt gcgcggctcc gggtcggcga ggccgcgcgt
      121 gaccagcgac agcgtcttgt cgttgatgag gctgccgagg atgtcgatgt actcggtggt
      181 ctggcgcttg tcggccgagg ccaacgcctc gaggatcttc ggaatggcgg cggtcccgag
      241 cttgccgagc ttggcgaaca gctgcttgcc ggtggccgag gaggggtccg attcctcacg
      301 aatctgcgcg atgagccgat cggcctgtag ggcggttatg aaattcatcg gcctcgcaga
      361 ctagggcgcc taattgaacc ggaaaatgct ccgacgaatc cgtcgaccgg cccgtttcct
      421 tggccggcga taaacataat ggtggtccca gcggtcgcaa ttatgccata cggttttcgc
      481 caaaatgtaa cgcgtatcac aggtttgcgg ggctctttag agtcgtgtct cgcggccggt
      541 tggccgaggc cattaggtta caatcgcacc ctacatccaa gtcgcccgcc atgtccgatt
      601 ccgcccgctc gaccctgcag gaagccctcg gcagcttcgt cgttccctat accggccggc
      661 ccctgggcgg gcccggggcc gcgtttacgc tcgagcgccg cggcagtgtg tggcacgtca
      721 cggtccagct cggcttcccg ctcgccgcga gcggcgaggc gcttgccgac gcggtgcgca
      781 tgcattgcgc cgcggcgctc gaaggcgcgt cgctcgagat cacgatcgaa agctcgatcg
      841 tcgcgcacgc cgtccagcac ggcctgaagc cgctgccggg cgctcacaat ctcatcgccg
      901 tcgcatcggg caagggcggc gtgggcaagt cgaccgtggc ggtgaatttc gcgctcgcgt
      961 tgaaggccga gggagccaag gtcgggttgc tcgatgcgga catctacggc ccgagccagc
     1021 cgcgcatgct cggcctcgtc ggccggcggc ccgagacccg cgacggcaag ctgctcgagc
     1081 cgctcgtcgc gcacgggatc gaggccatgt cgatcggctt cctcgtcgac gagcagcagc
     1141 cgatggcgtg gcgcgggccg atggtgacgt cggcgctgaa tcagctgctg acgcagacgc
     1201 gctggggcga tctcgactac ctgatcgtcg acatgccgcc cggcacgggc gacattcagc
     1261 tgacgctcgc gcagcgcgtg cccgtcagcg gcgccgtcat cgtcacgacg ccgcaagaca
     1321 tcgcgctcgc cgacgcgcgc aaagggctcg agatgttcca gaaggtgcac gtgcccgtgc
     1381 tcggcgtcgt cgagaacatg agcctgcaca tctgcagccg ctgcggccac gaggagcaca
     1441 tcttcggcga gcacggcggc agaaatttgg ccgagcaata cggcgtgccg ctgctcggcg
     1501 cgttgccgct cgaccgccgc atccgcgagg agacggatca gggcaaaccg accgtcgcgg
     1561 cggacccgaa gagcccgctc gcccgggcct tcaccgaggc ggcgctgcgt gccgccggcg
     1621 agctcgcggc gcgcgcgaaa gactacagcc gtctctttcc gaatatcacc gtcgaggaca
     1681 attgatgagc gtgaaggccg accgttggat tcgccgcatg gcggcgcagc acaagatgat
     1741 cgagccgttc gaggccggcc aggtgcgtca agtcgacggc aaccgcgtca tctcgttcgg
     1801 cacgtcgagc tacggctacg acgtgcgctg cgcgcccgag ttcaaggtct tcacgaacat
     1861 caactcggcg gtcgtcgacc cgaagaagtt cgtcgagtcg agcttcgtcg acatggcggg
     1921 cgacatttgc atcatcccgc cgaactcgtt cgcgctcgcg cgcaccgtcg agtacttccg
     1981 catcccgcgc aacgtgctga cgatttgcct cggcaaatgc gtcaccggag atacgcgggt
     2041 cgtggacgcc gagaccggcg cgctcgttcc aattcgagaa ttcaccgagc ggcacaggac
     2101 cgtcggtatg gaacactgga ggctctccgg acaaagcgtc gctgccgtga tgcctcgtgg
     2161 tcgcaagccc gtgttcgagc tcacgactcg tgcgggtctg aggatacgcg cgacggacaa
     2221 ccacccattc cgtcagctcg cgggttggac gccgctcaat caattgcggg cgggagatcg
     2281 aatcgccgta gccagacgga ttccgatttt cggcgagact ccgctacccg agtgggaggc
     2341 gacccttctc ggcctgatga tctcggaagg gcagtgttgc acaccgggct acagtccggt
     2401 ttttacgagc accgatcccg ttctcgtcga gcacttacgg caggccgttc acgccggcgg
     2461 cctcggcgag gttacgcaca gtggtgtagc gggctatcgt ctcgtgaacc acaagaaccg
     2521 aggcggcgtg cagcacaaga atcgcgccgc gcaatggctc gaacttcacg gaatcaacgt
     2581 caaggccgga aacaagctcg ttccacaggc cgtattcacc gcgccgcggc agtccacccg
     2641 gctctttctg cagtccctgt tctcaggcga cggtggcctt tacagaagcg gcgaaggctt
     2701 tttcttggag tactactcga agtccagacg gctgatcgag gatgtgcatc acttgctgct
     2761 ccggtttgga attttctcgc tgattcgtga aaagcaaacc aagatcggta cgagggcttg
     2821 cagcattcag ataaccgatc gagaggagat ccttcgtttc agcgacgagg tcgggtttct
     2881 accgggctcg atcaaacaac tgcgcctcga gcgcgagatt cttccgctgc tgcgcgcgca
     2941 acccgttcgc ctgcgcagta acttcgacac gttgccgcgc gaggcctgga cgctgctgac
     3001 cgcggtggcc gcgactcacg gtcgaagttt ccgttcgatg ggacttagca agtctgcgag
     3061 ccagtcggtg ccttacaaac acgtctcgca gatcgcgcta ctcactgcaa gctacgagct
     3121 cgcgcagctt ctcgatggtc ctgtttggga cgtcgtgaca gagatcagtc cggcgggaga
     3181 ggaggaggtg ttcgatttgt cggtgcctgc cgtcgagaac ttcgtcgcga acgacttcat
     3241 cgttcacaat tcaacgtatg cgcgttgcgg aattattgtg aacgtaacgc ccttggaacc
     3301 cgagtgggaa ggtcacgtca cgctcgagtt ctcgaacacg acgacgctgc ccgcgaagat
     3361 ttacgccaac gagggcgtcg cgcagatgat cttcttcgag tcggacgagg tctgcgagac
     3421 gagctacgcc gatcgcggcg gcaagtacca ggggcaacgc ggcgtcacgt tgccgcgaac
     3481 gtaagacccg gccgtcaccg actaggcgcg aggccgtcta cggtctgcag caagaacgag
     3541 gtttggacct cgtcgttgcg acgctgctcg atgcgtacgg gcaagaaact cagtttcggc
     3601 gcgacccaca gccaggtcga gcgcgacgag ccctcgcggt gctggacgaa caattgtgtc
     3661 tccagcgagc cgacgccggt ctgcaacgtc gccgggccgc tgtcggtgta ctcgtaggtg
     3721 ccgaccgagt cctcgtcggc aatttggtag gggcccgggg ctcccttgaa ggccatgtcg
     3781 cgcatgaccg ccgccttcac gg
//
LOCUS       DASSNN010000259         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1670905, whole genome shotgun sequence.
ACCESSION   DASSNN010000259 DASSNN010000000
VERSION     DASSNN010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670905"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VFL84_04670"
     CDS             <1..1293
                     /locus_tag="VFL84_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607946.1"
                     /translation="AGVLCGVLPALASGSSKAIGALRDGGRNATLSRARHRTRNVLVV
                     TQVALAFALVIGSGLMVRSFDALRSVDPGFQADGVLTFNVRPLPTKYAGPEPLARFYD
                     SLIERLEAVPGVTRAGGINALPLGAGTGNLATVIEEFPPAEGEFPPGFFIYRATPGYF
                     EAMSIPVVEGRSLTPDDHHNRLGSVIINSSLKERYWPSTSALGKRLNISGIRAQVVGV
                     VGDVHTAGLDVEAPQVLYLPMLDAVGANALMAVGGMTITVRTSVEPLSVVPAIRAAIA
                     ELDADLPIANVRSMQDVVGTSLSRSSFMMTLLVIAALIALFLGSIGIYGVLSYIVTTR
                     TAEIGVRSALGASPERLRRLVLFQGLRLAGVGVLIGVAGAIVLGRVIEAQLYGVEPVD
                     PVTLGAGAAIFLAVAVLASLLPAIRAARTAPVDALRAG"
     gene            1320..2222
                     /locus_tag="VFL84_04675"
     CDS             1320..2222
                     /locus_tag="VFL84_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607947.1"
                     /translation="MSTGSASGEIEALFKLPLGEFTSARNALAAQLKKAGRAEEANAV
                     KALAKPSASAWVVNQLYWRHRESFSRLLAAGEKLRRAHAAQLTGDAVNARREAMTELT
                     KLAELIVMRDGTHSATRDLMRRVTSTLEALSSYASSPEAPIAGRLTDDLQPPGFEAVA
                     GLLPQSGKARGQAKMRLPHAQPATNRASNVAAARRDEKGRKELVAAAKTAVRDAQRAL
                     NAARRQAERAAAKAEIAAKRAKQIEAQRAQIEKQLARASADAQAARAGASEATTAVNE
                     ATKSVESAERALELARQRLQQMLG"
BASE COUNT          349 a          747 c          819 g          344 t
ORIGIN      
        1 gccggcgtgc tttgcggtgt gttgccggcg ttggcgtcgg gctcgagcaa ggcgatcggt
       61 gcgcttcgcg acggcggccg caacgctacg ctcagccgcg cgcggcatcg cacacgaaac
      121 gtgctcgtgg tgacgcaggt cgcgctcgcg tttgcgctcg tgatcggctc gggactgatg
      181 gtccgaagct tcgacgcgct gcgctccgtc gatcctgggt tccaagccga cggcgtgctg
      241 acgttcaacg tgcggccgct gccgacgaag tatgcgggac cggagccgct cgcgcggttc
      301 tacgactcgc tgatcgagcg gctcgaagcc gttcccggcg tgacgcgcgc cggcgggatc
      361 aacgcgctgc cgctcggcgc cggcaccggc aatctggcga ccgtgatcga ggagtttcct
      421 ccggccgagg gcgagtttcc gccgggcttc ttcatctata gagcgacgcc gggctatttc
      481 gaggcgatga gcatcccggt cgtcgaaggt cgttcgttga cgccggacga tcaccacaac
      541 cgcctcggct ccgtcatcat caacagctcg ctgaaggagc ggtactggcc gagcacgagc
      601 gcgctcggta agcggttgaa catctcgggt attcgagcgc aagtcgtcgg ggtcgtagga
      661 gacgtgcaca cggcggggct cgacgtcgaa gcgccgcagg ttctctacct gcccatgctc
      721 gacgcggtgg gcgccaatgc actgatggcg gtggggggca tgacgatcac ggtccgcacg
      781 tcggtcgagc cgctcagcgt cgtgcccgcg attcgcgccg cgatcgccga gctcgacgcc
      841 gacctgccga tcgcgaacgt ccgctccatg caagacgtcg tcggcacttc gttgagccgc
      901 agcagcttca tgatgacgct gctcgtgatc gccgccctga tcgcgctgtt cttgggatcg
      961 atcggcattt acggcgtgct gtcctacatc gtgaccacgc gcacggccga gatcggcgtg
     1021 cggtcggcgc tcggcgcgag ccccgagcgg ctgcgccgtt tggtgctctt tcaagggctg
     1081 cggctcgcgg gcgtgggcgt gctgatcggc gtcgcgggcg caattgtgct cggccgcgtg
     1141 atcgaggcgc agctctacgg cgtcgagccc gtcgaccccg tcacgctcgg cgcgggcgcg
     1201 gcgatcttct tggccgtcgc cgtgctcgca agcctgctgc ccgcgatccg tgccgcgcgc
     1261 acggcgccgg tcgacgcgct gcgcgccggc tgacgatccc ggaccgtggc gcgtcagccg
     1321 tgagcacggg ctcggcctcc ggcgagatcg aggcattgtt caagctgccg ctcggcgaat
     1381 tcacgtcggc gcgcaacgcg ctcgccgcgc agctgaagaa agcggggcga gcggaagagg
     1441 cgaacgcggt caaggcgctc gcgaagccgt cggcctccgc ctgggtcgtc aatcagctgt
     1501 actggcggca tcgcgaatcg ttcagccggc tgctcgcggc gggcgaaaag ctgcgtcgcg
     1561 cgcatgcggc gcagctgacg ggcgatgccg tcaacgcgcg gcgcgaggcg atgacggagc
     1621 tcacgaagct cgccgagctg atcgtgatgc gcgacgggac tcacagcgcg acgcgcgacc
     1681 tgatgcgccg cgtgacgagc acgctcgagg cgctgtcgag ttacgcgtcg tcgccggaag
     1741 cgccgatcgc gggacgtctc acggacgact tgcagccgcc gggattcgag gcggtcgcgg
     1801 ggcttctgcc gcagagcggc aaagcgcgcg gccaggcgaa gatgcgattg ccgcacgcgc
     1861 aacccgcgac gaaccgcgcg agcaacgtgg ccgctgcacg ccgtgacgag aaaggtcgaa
     1921 aggagctggt cgcagcggcc aaaaccgccg tgcgcgacgc gcagcgcgcg ttgaacgcgg
     1981 cgcgtaggca agccgagcgc gcggcggcaa aggccgagat tgccgcgaag cgtgcgaaac
     2041 agatcgaggc gcagcgcgca cagatcgaga agcagctcgc ccgcgcgtcg gccgacgccc
     2101 aagcggcgcg agcgggcgcg agcgaagcga cgactgccgt caacgaggcg acgaaatccg
     2161 tggaaagcgc cgagcgcgcg ctagagcttg cgcgccaacg gttgcagcag atgctcggat
     2221 agtctcttgc cttatgtccg cacgctgccg ttggcagcc
//
LOCUS       DASSNN010000260         2971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_49084, whole genome shotgun sequence.
ACCESSION   DASSNN010000260 DASSNN010000000
VERSION     DASSNN010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49084"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1611
                     /locus_tag="VFL84_04680"
     CDS             37..1611
                     /locus_tag="VFL84_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7607948.1"
                     /translation="MQLTQRFVGAFLIALVCTAGAVQAQPASDARQVVTRAAEALGGA
                     QRIQALRTMRLRGYGHDQYQDGGSLITTEPTAPEKMTNISAYERVIDLANNRTRVRAK
                     QSRAFVFAAQAMMEGRPVDQALDGDVGFDVGAGGAARRQSAETAMRRRMELNANPIVA
                     VRAALDSRSRLSNRRTEGAATLVDVATAAGERFTLAVTTSTGLPLWVRWVGPHENLGD
                     LTYRAEFSGYEPVDGVWLPMSFNVVSDWKDNIQMRLHVDRYVLDGDVGDLAAPAAVRS
                     APAPTPSYTVDASVVAPGVWLLSGNGGANSVLLEFADHLSLFEVPTNRAWTAALIAKA
                     RSTVPGKPVTEAIISHHHFDHTGGLRTAIAEGLTIVTQAGNVAWFEELARRKVEAFPD
                     ALSRSPKPIKIRAVDDHLKLSDSKMTVDVYRVVSNGHMAHGLMAYIPEHRLLIQGDLF
                     DVGWEVYFWGNTYDDNVAYRKLDVERDVPIHGRVLPIAEVHAKLAEQTRNAEQLCAKV
                     ASVNLSMPGCPLAWND"
     gene            1613..>2971
                     /locus_tag="VFL84_04685"
     CDS             1613..>2971
                     /locus_tag="VFL84_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7607949.1"
                     /translation="MRLAGIAALALLALPVAAAASPEAERVMADVARAMGGAEAILRI
                     RTLQADGYGMEAYFWGGGNITGDREAPQKWAENPNFSSIWDFESDRYRTQYRHNFLFP
                     FGGTFGHSFALSAWGVDGDVGYTLAPGGAGTRLAEWTTGGAWFKPDGTVFRKYESLSH
                     PLAAVRAVLSRKAAPENLRIENGMPVVDLALDEGYVTLGVDPGTMLPRWVRWTAPHQN
                     LGEVVITSTFVGYQNFDGVQLPFTWVARIDWRNTLIQTRILDGYYVNSNKTPDIAAPA
                     AVRAQPLPPAAPPAAPITVTKIADGLWHLNPGGHTVIEFEDHLVIFEMGGTAAQARAA
                     IALANTLVRGKPLTHLIVSHHHFDHTLGFRAAVEAGLTVISHWGNEEILTDMATRPAP
                     NFAGLVPNPNGAPLKFVPVRGRLRLEDKRMTLDIYEVVKHNHMANAVFAYAPKSRTLM
                     EGD"
BASE COUNT          505 a          999 c          996 g          471 t
ORIGIN      
        1 cgccggcagt caactgatcc tcggggggag gttcgaatgc aattaacaca acgattcgtc
       61 ggcgctttct tgatcgcctt ggtttgcacg gccggcgccg tgcaggctca gcccgcctcg
      121 gacgcccgcc aggtcgtgac acgcgcggcc gaagctctcg gcggcgcgca gcgaattcaa
      181 gcgctgcgca ccatgcggct gcgcggctac ggccacgacc agtatcaaga cggcggcagc
      241 ctgatcacca ccgagccgac tgcgcccgag aagatgacga acatctcggc ctacgagcgc
      301 gtcatcgatc ttgcgaacaa ccgcacgcgc gtgcgggcga agcaatcgcg cgccttcgtg
      361 ttcgccgcgc aggcgatgat ggaagggcgc cccgtcgacc aggcgctcga cggcgacgtc
      421 gggttcgacg tcggggccgg cggcgccgcg cggcggcaat ccgccgaaac cgccatgcgc
      481 cggcgcatgg agctcaacgc caacccgatc gtggccgtgc gcgcggcgct cgactcgcgc
      541 agccgcctct cgaaccggcg cacggaagga gcggcgacgc tcgtcgacgt tgcgacagcg
      601 gcgggcgagc gcttcacgct ggccgtcacg acttcgaccg gcttgccgct gtgggtgcgg
      661 tgggtcgggc cgcacgagaa tctcggcgac ctgacctatc gcgccgagtt cagcggctac
      721 gagcccgtcg acggcgtgtg gttgccgatg agcttcaacg tcgtgtccga ttggaaggac
      781 aacatccaga tgcgcctgca cgtcgaccgt tacgtgctcg acggcgacgt cggcgacctg
      841 gccgcaccgg ccgccgtgcg ctctgcgccg gcgccgacgc cgagctatac ggtcgacgcg
      901 agcgtcgtcg cccccggcgt ctggctgctc tcgggtaacg gcggcgcgaa ctccgtgctg
      961 ctcgagttcg cggaccacct gagcctgttc gaggtgccga cgaatcgcgc gtggaccgcg
     1021 gcgctgatcg ccaaggcgcg cagcacggtg ccgggtaagc ccgtgaccga agccatcatc
     1081 tcgcaccatc acttcgatca caccggcggg ctccgcaccg cgatcgcgga gggcctcacg
     1141 atcgtgacgc aagccggcaa cgtcgcgtgg ttcgaggagc ttgcgcggcg gaaagtcgaa
     1201 gcgtttccgg atgcgttgag ccgtagcccg aagccgatca agattcgcgc ggtcgacgat
     1261 cacttgaagc tcagcgactc gaaaatgacc gtcgacgtct atcgcgtcgt ctcgaacggc
     1321 cacatggcgc acggcctcat ggcgtacatc cccgagcacc gtttgttgat ccagggcgac
     1381 ctgttcgacg tgggctggga agtctatttc tggggcaaca cctacgacga caacgtcgcc
     1441 tatcgcaagc tcgacgtcga gcgcgacgtg ccgatccacg ggcgcgtgct gccgatcgcc
     1501 gaagtgcacg cgaagctggc cgagcaaaca cgcaatgccg agcagctctg cgcgaaggtc
     1561 gcatcggtga atctgtcgat gccgggctgc ccgctcgcgt ggaacgattg aagtgcgcct
     1621 agccggaatt gccgcgctcg cgctgctcgc gttgcctgtg gccgccgcgg cgtcacccga
     1681 ggccgagcga gtcatggcag acgtggcgcg cgcgatgggc ggggcggagg ctatcctgcg
     1741 tatccggacg ttgcaggccg acggctacgg catggaggcc tatttctggg gcggcggcaa
     1801 catcacgggc gatcgcgaag ccccgcagaa atgggccgag aacccgaact tctcgtcgat
     1861 ttgggacttc gagagcgatc gctaccgcac gcagtatcgg cacaacttcc tgtttccgtt
     1921 cggcggcacg ttcgggcaca gcttcgcgct gtcggcctgg ggcgtcgacg gcgacgtcgg
     1981 ctatacgctc gcgccgggcg gcgcgggcac tcggctcgcc gaatggacga cgggcggggc
     2041 ctggttcaag ccggacggca cggtgttccg caagtacgag tcgttgagcc acccgctggc
     2101 ggccgtgcgc gccgtgctga gccgcaaggc agctccagaa aacctgcgca tcgagaacgg
     2161 catgcccgtc gtcgatttgg ctctagacga aggctacgtc actctcggcg tcgatcccgg
     2221 caccatgttg ccgcgttggg tgcgctggac ggcgccgcac cagaacctcg gcgaggtcgt
     2281 catcacatcg accttcgtcg gctatcagaa tttcgacggc gtgcagttgc cgttcacttg
     2341 ggtcgcgcgc atcgactggc gcaacacgct catccaaacg cggattctcg acggctacta
     2401 cgtgaactcg aacaagacgc ctgacatcgc cgcgcccgcg gctgtgcgcg cgcagccgct
     2461 gcctccggcg gccccacccg ccgcgccgat cacggtgacg aagatcgccg acgggctgtg
     2521 gcacttgaac ccgggcggcc acacggtcat cgagttcgaa gatcatctcg tgattttcga
     2581 gatgggcggc acagccgcgc aggcacgggc ggccatcgcg cttgcgaaca cgctggtgcg
     2641 cggcaagccg ctcactcacc tcatcgtctc gcatcaccat ttcgatcaca cgctcggctt
     2701 tcgcgcggcc gtcgaggccg ggctgaccgt gatctcgcac tggggcaacg aagaaatcct
     2761 gaccgacatg gcgactcggc cggcgccgaa tttcgccggg ctggtgccga atccgaacgg
     2821 cgcaccgctg aagttcgtgc cggtgcgcgg ccggctgcgc ctggaagaca agcgcatgac
     2881 gctcgacatc tacgaagtgg tcaagcacaa ccacatggcg aacgcggtgt tcgcgtatgc
     2941 gccgaagagc cgcacgctca tggaaggcga c
//
LOCUS       DASSNN010000261         2180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1290142, whole genome shotgun sequence.
ACCESSION   DASSNN010000261 DASSNN010000000
VERSION     DASSNN010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290142"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFL84_04690"
     CDS             complement(<1..713)
                     /locus_tag="VFL84_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7607950.1"
                     /translation="MRAPLRIAAVAALAVALVACAGRKEKGEPPAKLVEFEPTLAIDK
                     VWSEKVGGKSERLRLGLRPATDGARIFAGAYDGQIASFDAATGDKVWSVKTNLPLTAG
                     PGFGDGLLAFGTADGDLLAVDAATGEERWREPIGSEVLAAPAIGSGVVVVRTVDGRLR
                     GFSAANGSMVWSAEQNLPTLTLRGNTAPRVSGPLVVSGFNNGRVAAYEVASGDPAWEV
                     AIANPTGRSELERLVDVSAG"
     gene            complement(710..1468)
                     /locus_tag="VFL84_04695"
     CDS             complement(710..1468)
                     /locus_tag="VFL84_04695"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7607951.1"
                     /translation="MDDYLTDKEQVERLRQWWRENGWFLIGGVALGGLAIVGYRQYFE
                     YRDAQSEQAAAMYQSVKQEADAGNTADAETRFATMRSEFPDNAYTHQAALLLASNEIV
                     TAPDDAAEKLRFTMENSDDPELAMVARLRLARVLAYRDRDEEALAVLNVPDPGQFAGR
                     IAEIKGDIHAANGETDAARTAYLEAMVAPGAELLDRGLLQMKLSDLPGAAPPPSAEAA
                     PATSAPDVALDAAEAPADAAPGETAPAQSKPEGE"
     gene            complement(1501..>2180)
                     /locus_tag="VFL84_04700"
     CDS             complement(1501..>2180)
                     /locus_tag="VFL84_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET7607952.1"
                     /translation="RNPLRILDSKNPALRELIEAAPLLSSYLDDASRAHFDRVQALLG
                     DVGVAYTLNPRLVRGLDYYSRTVFEWLTDRLGAQSAICSGGRYDGLVAQLGGRDTPAV
                     GWALGVERIVELMQAEGIAAGEAALDAQLVAAGDAERRFGFKLVDQLRERLPGVRIAL
                     GVPSAGFKAQLRHADKSGARFALIVGESELAAGKVAVKPLRDGKPQRLATVEECADLL
                     LETPGSA"
BASE COUNT          315 a          790 c          732 g          343 t
ORIGIN      
        1 ccggcgctga cgtcgacgag gcgctcgagc tcgctgcggc ccgtcgggtt cgcgatggcg
       61 acctcccatg ccggatcgcc gctcgcgact tcataggccg cgacgcggcc gttgttgaag
      121 ccgctcacga cgagcgggcc cgagacgcgc ggcgccgtgt tgccgcgcag cgtcagcgtc
      181 ggcaggttct gctcggcgct ccagaccatc gagccgttcg cagccgaaaa gccgcgcaag
      241 cgcccgtcga cggtgcgcac gacgacgacg ccggagccga tcgccggcgc cgcgagaact
      301 tcgctgccga tcggttcccg ccaccgctct tctcccgtcg cggcatcgac cgcgagcaag
      361 tcgccgtccg ccgtgccgaa tgcgagcagc ccgtcgccga agccggggcc tgccgtcagc
      421 ggcaggttcg tcttgacgga ccagaccttg tcgccggtcg cggcatcgaa cgacgcgatt
      481 tgaccgtcat aagcgcccgc gaagatgcgc gcgccgtcgg tcgcgggacg caagccgagc
      541 cgcaagcgct cggacttgcc gccgactttc tcgctccaga ccttgtcgat cgcgagcgtc
      601 ggctcgaact cgacgagctt cgcgggtggc tctcccttct ctttacggcc cgcgcacgcg
      661 accagcgcga cggcgagcgc cgcgactgcg gcgatgcgca gcggcgctct cattcgccct
      721 ccggcttcga ctgcgcgggc gcagtctcgc cgggtgccgc atcggcgggt gcctcggccg
      781 cgtcgagcgc gacgtccggg gcggacgttg ccggagccgc ttcggcggac ggcggcggcg
      841 cggcgccggg caagtccgaa agcttcatct gcaagaggcc ccgatcgaga agctcggcgc
      901 ccggcgcgac catcgcttcg aggtacgccg tgcgcgcggc atcggtttcg ccgttcgccg
      961 cgtgaatgtc gcctttgatc tctgcaatgc ggccagcgaa ctgacccgga tcgggaacgt
     1021 tcaacacggc gagcgcctcc tcgtcgcggt cgcggtacgc gagcacgcgc gcgagcctga
     1081 ggcgcgcgac catcgcgagc tccgggtcgt cgctgttctc catcgtgaag cgcagcttct
     1141 cggccgcgtc gtcgggcgcc gtgacgatct cgttgctcgc caacagcagc gcggcttgat
     1201 gcgtatacgc gttatcgggg aactcgctgc gcatcgtcgc gaagcgtgtc tcggcatccg
     1261 ccgtgttccc agcgtcggcc tcctgcttca ccgactggta catcgcggcg gcctgctcgg
     1321 attgtgcatc gcggtattcg aagtattggc ggtagccgac gatcgccagg ccgcccagtg
     1381 ccacgccgcc gatcaagaac cagccgttct cgcgccacca ttggcgcaag cgctcgactt
     1441 gttctttatc cgtcaaatag tcgtccacac gatcctccgg ccggcaatga gcgggctatt
     1501 ctatgccgag ccaggcgtct caaggagcag gtcggcgcac tcttcgaccg tggcgaggcg
     1561 ctgaggttta ccgtcgcgca acggctttac ggccaccttc ccggcggcaa gctccgattc
     1621 gccgacgatc agggcgaacc gcgccccgct cttgtccgca tgacgaagct gcgctttgaa
     1681 gccggccgac ggcaccccga gcgcgatccg gacgcccggc aaccgctcgc gaagttggtc
     1741 cacgagcttg aagccgaacc gccgctccgc gtcgccggcc gcgacgagct gcgcgtcgag
     1801 cgcggcctcg cccgcggcga tgccctcggc ctgcatgagc tcgacgatgc gctctacgcc
     1861 taacgcccag ccgacggccg gcgtgtcgcg cccgccgagc tgggccacga ggccgtcgta
     1921 gcgaccgccc gagcagatgg cgctctgcgc gccgagccga tccgtgagcc actcgaacac
     1981 cgttcgcgag tagtagtcga gcccgcgcac gagccgcggg ttcagcgtgt acgcgacgcc
     2041 gacgtcgccg agcagggctt gcacgcgatc gaagtgcgcg cgcgacgcgt cgtcgagata
     2101 gctcgacagc agcggcgcgg cctcgatgag ctcgcgcaac gccggattct tgctgtcgag
     2161 gatccgcagc ggattgcgct
//
LOCUS       DASSNN010000262         6916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_283545, whole genome shotgun sequence.
ACCESSION   DASSNN010000262 DASSNN010000000
VERSION     DASSNN010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283545"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFL84_04705"
     CDS             <1..346
                     /locus_tag="VFL84_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607953.1"
                     /translation="LLGRDELIGAVPGEVLELRIVGESDYQTVECLIELRDEARPAAS
                     FAATPAGNVNCTSTRLASRWRARCARFARRNCSRRGTLLSGCALQLACARALRYDPRR
                     PPRGAPQPEKTA"
     gene            343..1098
                     /locus_tag="VFL84_04710"
     CDS             343..1098
                     /locus_tag="VFL84_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7607954.1"
                     /translation="MRILIGTLLLAAGAGSPAAGQHATAFDVQDGRRVYEGLCAACHG
                     PDGNLIAGIDFSRGVFRRTFSDDELAATIMAGIPNTPMPPNPTMRRDEAVRVVEYLRS
                     MSAGRETTADGDATRGRALFEGKGECTDCHAVRGVGSRVGPDLTRIGAVRRAGELERS
                     LLDPKAEVQPENRFYKVTPKGGKPVVGRLLNRDTYTVQLMDLDERLRSFRIADLAAHD
                     FDDTPMPSAREKLSTREIADVVAYLSSLRGATP"
     gene            1095..2705
                     /locus_tag="VFL84_04715"
     CDS             1095..2705
                     /locus_tag="VFL84_04715"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HET7607955.1"
                     /translation="MRTIVLATFAVCLPLHAQVTFERIVNAEREPQNWLSYSGTVDNQ
                     RYSLLDQIDTQTVDELTLQWVWQARSLEKFEATALVVDGILYTVQAPNDVVALDAATG
                     RVFWTYHHEPAPEGRPCCGRVNRGVAILGDTLYMGTIDAHLLAIDAKSGKLIWDSVVA
                     DAKRNYSITMSPNVFKDKVIVGTGGGDMGIRGYIAAFDVKTGKEAWRFYTIPGPGEPG
                     HDSWSGESWRTGGAAVWNAAAYDPETNLAFWGTGNPAPDWDGSGRLGDNLYSNSVVAL
                     DVDTGELKWHYQFTPHDELDYDSTQVPVLADIDWRGTPRRVMLWANRNGILYALDART
                     GELLAGKPYVVHNWFGGFDANGRLQRKPGMEIDKTAKLFRPHVHGAINWAPTSFSPRT
                     GLYYVSHWENSGIVAMEGLFPQPVGINRRQTGMGQVNLEPFFNNNDEAYGVIRAYDPH
                     TLEAKWEHRMADITWAGVLSTAGDLVFGGGREGYFLALDARTGALLWRASLGGQINAG
                     AMSYAVNGRQYVAIAAGSALFSFALPEK"
     gene            2712..3590
                     /locus_tag="VFL84_04720"
     CDS             2712..3590
                     /locus_tag="VFL84_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET7607956.1"
                     /translation="MAQRDVLEVGVDELRPGRYQPRLQLREDGLADLADSIREQGVLQ
                     PLIVRAAPATEGDASAYEIVAGERRWRAARLAGLATVPAIVRELTDRETLAVALIENL
                     QREDLNPIDQAQSMVRLVEEFGMTHDQIAKALGRSRAGVTNFLRLLELADGVKDAIVA
                     GTLDMGHARALLPLDSHRQVRLARKIVRLGWSARKVEQTVKALLERPAGVKRRTPVDI
                     QTRWLERQLARELGEKISIRPGPSGGYMLQLGFADLASLDLSLERLHELVRQVRAAAG
                     PRAREGARDSKAQGES"
     gene            complement(3587..3943)
                     /locus_tag="VFL84_04725"
     CDS             complement(3587..3943)
                     /locus_tag="VFL84_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET7607957.1"
                     /translation="MSKAKAYIKVGCPFSFKFLTFMAEAKLLDEIEIVRVREGDGDYE
                     ATKQKLAEHLGKASFPTVEVEPNKYMSDSDRLIEHYAKRAGLRPDEMPVLSLYKEGIL
                     PKLFELHKLKTGGAKA"
     gene            complement(4006..4158)
                     /locus_tag="VFL84_04730"
     CDS             complement(4006..4158)
                     /locus_tag="VFL84_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607958.1"
                     /translation="MIRKLPSGEYRLYSRKRDPRTGKRRNLGTFDTREAAERHERAVQ
                     YFKRRH"
     gene            4232..4645
                     /locus_tag="VFL84_04735"
     CDS             4232..4645
                     /locus_tag="VFL84_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017626738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HET7607959.1"
                     /translation="MPGREAMPSTIRRSPAKAQRTWIKVHDAAAKEYGDGERAHRTAY
                     AALKHSFERRGDRWVPKDAKGPSDPRAKQTRSAARKGRGKTFGGVDYYGHSKRELYAR
                     AKKLGVPGRSTMSKDELASAIARRQKSRSRSAQRH"
     gene            4664..5428
                     /locus_tag="VFL84_04740"
     CDS             4664..5428
                     /locus_tag="VFL84_04740"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET7607960.1"
                     /translation="MSEQAIHRSALFFMAVVAAVTANLWAQHDPAPAAQRTADTTLAE
                     WLNDKDARLSELIGRRVMNPSGEDLGEVEDVLATPSRDQRPVVVLSIGGVLDVGDKWH
                     AASLDQLRIAPDNERLLLDKTKAELKAAPGFDYVPMIGERSHQPGVRGPNTTNSLGRL
                     LGATVVDDAGDPIGEIKDFVVSTGEQGTRAVVGLDDSAGAAEGRLVAVPFDALHIERS
                     GEEARAVPQMARVRVELRGKPLDTLPAYEYPLREAI"
     gene            complement(5425..6336)
                     /locus_tag="VFL84_04745"
     CDS             complement(5425..6336)
                     /locus_tag="VFL84_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607961.1"
                     /translation="MGTDSRDWAHHELDEAARAALNEGLAASALWSVLLGVMERRARR
                     KPAALLEQWRQDRFVQPCAIDQRTLHELDSHLLAAASAFEAIELAPLAPLGVCSSVAV
                     TSQNRIVSTARGTEVVSDPTNVLALESVRRLRENPAAEIKLATSHRCTRAQAVPDKPG
                     FAAHFRIFCLTSAAHERKDHAFVTDALHEHIRTHCAALERLEQHGYSFAERTVKLLST
                     PARRHLAERIAAALSGIPVTHAELEHGYYDGLRFMIDVTAPDGARIPLVDGGAFDWLR
                     TLGANDKLTFVASGMGSQVAAYAFRRS"
     gene            6647..>6916
                     /locus_tag="VFL84_04750"
     CDS             6647..>6916
                     /locus_tag="VFL84_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidase"
                     /protein_id="HET7607962.1"
                     /translation="MGTVAAACALALPVAAQPNVDGLWSQVYNWPLIAVHAAMTPEGR
                     VLTYGTKSDGTQTGFFSYDLWDPTAGPSGGHTTFNNGTGTDIFCSS"
BASE COUNT         1164 a         2315 c         2357 g         1080 t
ORIGIN      
        1 cctccttggc cgtgatgaac tcatcggtgc ggttccaggc gaggtccttg agctgcggat
       61 agtcggcgag tcggactacc aaacggtcga gtgcctcatc gagctgcgcg acgaagctcg
      121 tcccgcggcg agcttcgccg cgacgccggc cggcaatgtc aactgcacct cgacgaggtt
      181 ggcgagccgt tggcgagcgc gttgcgcgcg cttcgcccgc cgcaactgct cgcggcgcgg
      241 caccttgctg agcggttgcg ccctgcagct cgcatgcgcg cgggccctac gctacgatcc
      301 tcgtcgccca ccgcggggcg cccctcaacc agagaaaaca gcatgagaat cctgatcggt
      361 accttgttgc tggccgcggg ggctgggtca cccgctgctg ggcagcacgc cacggcgttc
      421 gacgttcagg acggccggcg cgtctacgag ggcttgtgcg cggcgtgcca cgggcccgat
      481 gggaacttga tcgccggcat cgacttcagc cgcggcgtct ttcgccgcac attctccgac
      541 gacgagctcg cggcgacgat catggccggc atcccgaaca cgccgatgcc gccgaatccg
      601 acgatgcgcc gcgacgaggc ggtgcgcgtg gtcgagtacc tgcgctcgat gagcgcggga
      661 cgggagacga ccgccgacgg cgacgcgaca cgcggccgcg cgttgttcga aggcaagggc
      721 gaatgcacgg attgccacgc cgtgcggggc gtgggctcgc gcgtcggccc cgatctcacg
      781 cgcatcggcg ccgtgcgtcg cgccggcgag ctcgagcgct cgctgctcga cccgaaggcc
      841 gaagtgcagc ccgagaaccg cttctataag gtgacgccga agggcggcaa gcccgtcgtc
      901 ggccgtctgc tgaatcgcga cacgtacacg gttcagctca tggatctcga cgagcggttg
      961 cggtcgttcc gcatcgccga tctcgccgcc cacgatttcg acgacacgcc gatgccgtcg
     1021 gcgcgcgaga agctatcgac gcgcgagatc gccgacgtcg tcgcctatct ttcttcgctg
     1081 cgaggcgcta ccccatgaga accatcgtcc tcgctacttt cgccgtttgc ctgccgctcc
     1141 acgcgcaagt cacgttcgag cgcatcgtca acgccgagcg cgagccgcag aactggttga
     1201 gctactcggg cacggtcgac aaccaacgct acagcctgct cgatcagatc gacacgcaaa
     1261 cggtcgacga gctcacgctg cagtgggtct ggcaagcgcg ctcgctcgag aagttcgagg
     1321 cgacggcgct cgtcgtcgac ggcattctct ataccgtgca ggcgccgaac gacgtcgtcg
     1381 cgctcgacgc cgcgaccggg cgcgtgttct ggacctacca ccacgagccg gcgcccgaag
     1441 gccgcccgtg ctgcggccgc gtcaatcgcg gcgtcgcgat cttgggcgac accttgtata
     1501 tgggcacgat cgatgcgcac ctgctcgcga tcgacgcgaa gagcggcaag ctgatctggg
     1561 acagcgtcgt cgccgacgcg aagcggaact actcgatcac gatgtcgccg aacgtcttca
     1621 aggacaaagt catcgtcggc acgggcggcg gcgacatggg aatccgcggc tacatcgccg
     1681 cgttcgacgt caagaccggc aaggaagcgt ggcgcttcta cacgatcccg gggcccggcg
     1741 agcccggcca cgactcgtgg tccggcgaat cgtggcgcac gggcggcgcc gccgtctgga
     1801 acgcggctgc gtacgatccg gagacgaatc tcgcgttctg gggcacgggc aatccggcgc
     1861 cggactggga cggcagcggg cggctcggcg acaatctcta cagcaactcg gtcgtcgcgc
     1921 tcgacgtcga caccggcgag ctgaagtggc actaccagtt cacgccgcac gacgagctcg
     1981 actacgactc gacgcaagtg ccggtgctcg ccgacatcga ctggcgcggc acgccgcgca
     2041 gggtcatgct ctgggcgaac cgcaacggca tcctatatgc gctcgacgcg agaaccggcg
     2101 agctcttggc cggaaagccc tacgtcgtcc ataactggtt cggcggcttc gacgcgaacg
     2161 gccgcttgca acgcaagccg ggcatggaga tcgacaagac ggcgaagttg ttccggccgc
     2221 acgtgcacgg cgcgatcaac tgggcgccga cgtcgttcag cccgcgcacg ggcctctact
     2281 acgtctcgca ctgggagaat tccggcatcg tcgcgatgga aggcttgttc ccgcagcccg
     2341 tcggcatcaa tcgccgccag accggcatgg gccaggtcaa cctcgagccg ttcttcaaca
     2401 acaacgacga ggcctacggc gtcatccgcg cctacgatcc gcacacgctc gaggcgaagt
     2461 gggagcaccg catggccgac atcacgtggg ccggcgtgct gtcgacggcc ggcgacctcg
     2521 tcttcggcgg cggccgcgaa ggctattttc tcgcgctcga cgcgcgcacg ggcgcgctgc
     2581 tctggcgcgc gtcgctcggc ggccagatca acgccggcgc gatgagctac gccgtgaacg
     2641 gccggcaata cgttgcgatc gcggccggca gcgcgctgtt ctcgttcgcg ttgccggaaa
     2701 aatagcgggc cgtggcgcag cgcgacgtat tggaagttgg tgtcgacgag cttcggcctg
     2761 ggcggtatca gccgcgcctc cagctgcgcg aagatggcct cgccgacctc gccgactcta
     2821 tccgcgagca aggggttctt cagccgctga tcgtcagggc cgctcccgcg acggaaggag
     2881 acgcgagcgc gtacgagatc gtcgccggcg agcggcgctg gcgcgccgcg cggctcgcgg
     2941 ggctcgcgac ggtccccgcc atcgtgcgcg agctcacgga tcgtgagacg ctcgccgtcg
     3001 cgctgatcga gaacctgcag cgcgaagatc tgaatccgat cgatcaagcg caatcgatgg
     3061 tgcggctcgt cgaagaattc ggcatgacgc acgaccagat cgcgaaagcg ctcggccgct
     3121 cgcgggcggg cgtcacgaac ttcctacggc tgctcgagct cgcggacggc gtcaaagatg
     3181 ccatcgtcgc cggcacgctc gacatggggc acgcgcgtgc gctgctgccg ctcgattcac
     3241 accggcaagt gaggctcgcg cgcaagatcg tgcggctcgg ctggtcggcg cggaaggtcg
     3301 agcagacggt caaggcgctg ctcgaacgcc cggccggcgt gaaacgcaga acgcccgtcg
     3361 acatccagac gcgctggctc gagcgccagc tcgcgcgcga gctcggggag aaaatctcga
     3421 ttcggccggg accgagcggc ggctacatgc tgcagctcgg cttcgcggac ctcgcgagcc
     3481 tcgacctcag cctggaacgg cttcacgagc tcgtgcggca agtccgcgcg gcggccggac
     3541 cgcgagcgcg agaaggcgct cgcgactcga aagcacaggg cgaatcctag gccttggcgc
     3601 cgcccgtctt cagcttgtgg agctcgaaga gcttcggcag gatcccctcc ttgtaaagcg
     3661 acagcaccgg catctcgtca ggccgcagtc ccgcgcgctt cgcgtagtgc tcgatgaggc
     3721 ggtccgagtc gctcatgtat ttgttcggct cgacttcgac cgttgggaac gacgctttgc
     3781 cgagatgctc ggcgagcttc tgcttcgtcg cctcgtagtc gccgtcgcct tcgcgaacgc
     3841 gcacgatctc gatctcgtcg agcagcttcg cctcggccat gaacgtcagg aacttgaacg
     3901 agaacggaca gcccaccttg atgtaggcct tggctttgct catttcgatg ctccctgcgt
     3961 tttcctggtt ttccggcgcg atttcgtcac ggcgtcaggt ccttgtcagt gtcgacgttt
     4021 gaaatattgc acggcgcgct cgtgccgctc ggcggcttcg cgcgtgtcga acgtgcctag
     4081 attgcgccgc ttgcccgtcc gcgggtcgcg cttgcgcgag tagagcctgt attcgccgga
     4141 agggagcttg cgaatcatgg cacgcatcct gcaagtcgcg tgccgatggg ccgcggactg
     4201 cggacggacg ttcgacagag gaggtcgagc catgcctgga agggaagcca tgccgagcac
     4261 gattcgccgc tcgccggcga aggcgcagcg tacctggatc aaggtgcacg acgcggccgc
     4321 caaggaatac ggcgacggcg aacgcgcgca ccgtaccgct tacgcggcgc tcaaacattc
     4381 gttcgagagg cgcggtgacc gctgggtgcc gaaagacgcc aagggcccgt cggacccgcg
     4441 cgcgaagcaa acgcgctccg cggcgcgcaa aggccgcggc aagaccttcg gcggcgtcga
     4501 ttactacggg cattccaagc gcgagctgta cgcacgcgcg aaaaagctcg gcgtccccgg
     4561 acggtccacg atgagcaagg acgagctcgc gagcgccatc gcgcggcggc agaagtcgcg
     4621 gtcgcggtcc gcgcaacggc attgagacga aaaggggaaa accatgagcg aacaagctat
     4681 tcatcgatct gcgttgtttt tcatggccgt ggtcgccgcg gtgaccgcga acctctgggc
     4741 ccagcacgat cccgcgcccg cggcgcagcg aaccgccgac acgacgctgg ccgaatggct
     4801 caacgacaag gatgcgcgcc tcagcgagct gatcggccgg cgcgtcatga acccgagcgg
     4861 cgaggacctg ggcgaagtcg aggacgtgct cgcgacgccg agccgcgacc agcgccccgt
     4921 cgtcgtgctg tcgatcggcg gcgtgctcga cgtcggcgac aagtggcacg cggcatcgct
     4981 cgaccagctg cgtatcgccc ccgacaacga gaggctgttg ctcgacaaga ccaaagccga
     5041 gctcaaagcc gcgccgggct tcgactacgt gccgatgatc ggcgagcgga gccaccagcc
     5101 cggcgtgcgc gggcccaaca cgacgaactc gttggggcgc ctgctcggcg cgaccgtcgt
     5161 cgacgatgcc ggcgacccga tcggcgagat caaagacttc gtcgtatcga cgggcgagca
     5221 gggcacgcgc gcggtcgtgg gcctcgatga cagcgcgggc gctgcggagg gccggctcgt
     5281 cgcggtgccg ttcgatgcgc tgcacatcga gcggagcggt gaggaagccc gggccgtgcc
     5341 gcaaatggcg cgcgtgcgcg tcgagctgcg cggaaaaccg ctcgacacgc tgcccgcgta
     5401 cgagtatccg ctgcgcgaag cgatctagct gcgccggaac gcgtatgccg ctacttgcga
     5461 tcccatgccg ctcgcgacga acgtgagctt gtcgttcgcg ccgagcgtgc gcagccaatc
     5521 gaacgcaccg ccgtcgacga gcggaatgcg cgcgccgtcg ggcgccgtga cgtcgatcat
     5581 gaagcgcagc ccgtcgtagt agccgtgctc gagctcggcg tgcgtgacgg gaatgcccga
     5641 cagcgctgcc gcgatccgct cggcgaggtg acgccgcgcc ggcgtcgaga gcagcttcac
     5701 cgtgcgctcc gcgaacgagt agccgtgctg ctcgaggcgc tcgagcgcgg cgcagtgcgt
     5761 gcggatgtgc tcgtgaagcg cgtcggttac gaacgcatgg tctttgcgct cgtgggcggc
     5821 cgatgtcagg cagaagattc ggaaatgcgc cgcgaagccc ggcttgtcgg gcacggcttg
     5881 cgcgcgcgtg cagcgatgcg acgtcgcgag cttgatctcg gctgcggggt tctcgcgtaa
     5941 ccggcgcacg ctctcgagcg cgagcacgtt ggtcggatcg gagacgactt ccgttccgcg
     6001 cgccgtcgag acaatgcggt tctggctcgt cacggcgaca ctcgagcaca cgccgagcgg
     6061 tgcaagcggc gcgagctcga tcgcttcgaa cgccgaggcc gcggcaagca aatgagagtc
     6121 gagctcgtgc agcgtgcgct gatcgatcgc gcacggctgc acgaaccgat cctgccgcca
     6181 ttgctcgagc agcgcagccg gtttgcgccg cgcgcgccgc tccatgacgc cgagcagcac
     6241 cgaccacagc gcggacgccg cgagcccttc gttcaaggcg gcacgagccg cttcgtcgag
     6301 ctcgtgatgc gcccaatcgc gcgaatcggt acccatggcg cggcatcatg gcgcctcgac
     6361 cgtcgttcga gaacgggtcc ggcgtcgaac tcttttacat cggcgctgat gttcgcttcg
     6421 caactggctg attctaggcg tgttcggttc gtggcgttct tcttgcatcg taggccgaaa
     6481 gagccctgcc gcgtgggtat ggcgtccaag gttgcggaga cgaccggggc gccaggggaa
     6541 cgaggcatag gggacccagg ggacgcccat gagcagcacg ccgcgcgaag ctctttcgcc
     6601 gcttatccac tcctgcacgc caaaacgacg atcgcgagcc ctcgctttgg gcacggtcgc
     6661 agccgcctgc gcgctcgcat tgcctgtggc cgcgcagccg aacgtcgacg gtctatggtc
     6721 gcaggtctac aactggccgc tgatcgcggt gcacgcggcg atgacgccgg aaggccgcgt
     6781 gctgacctac ggcacgaaga gcgacggcac gcagacggga ttcttctcgt acgacctctg
     6841 ggatccgacg gcggggccga gcggcggcca cacgacgttc aacaacggca ccggcacgga
     6901 tattttctgc agctcg
//
LOCUS       DASSNN010000263         4888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_49855, whole genome shotgun sequence.
ACCESSION   DASSNN010000263 DASSNN010000000
VERSION     DASSNN010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49855"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VFL84_04755"
     CDS             <1..680
                     /locus_tag="VFL84_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HET7607963.1"
                     /translation="LGRARVLVVGLGGLGCPAALYLATSGVGKLVLNDFDRVDETNLP
                     RQILYAPEDVGALKVEAAKRRLTALNPGVEVVVLPERLDGAALEAAVGDVDIVLDGTD
                     NFAVRFAVNRACVARRVPLVSGAAVRVEGQIAVFPNDDDGPCYACLYDDEDEWLGDCQ
                     GNGVLAPVPGVIGTLMAEEALKLALGWDSSLRNRLLLWDAKRGEWQSVALKRDPDCVV
                     CGRGRVT"
     gene            1032..1370
                     /locus_tag="VFL84_04760"
     CDS             1032..1370
                     /locus_tag="VFL84_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HET7607964.1"
                     /translation="MKTTSRSVLALATLLASLGWSATALAAPTRAGEVATKVVRFKDL
                     DLSTAEGAQSLYERIKMAARIVCREAPHAQERACRTRAVDDAVRMVGRPLLSLVHRSM
                     VDRVEEVVLR"
     gene            complement(1465..2832)
                     /locus_tag="VFL84_04765"
     CDS             complement(1465..2832)
                     /locus_tag="VFL84_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET7607965.1"
                     /translation="MAIQFSPDEILETIRMVQMENLDIRTITMGISLRNCADASLAAA
                     AKKSYDKICRSAARLVAVGEEIEREYGIPIINKRVSVTPVALVAEASASGDYVLFAEH
                     LERAAREIGVNFLGGFSALVHKGTTPGDDALISSIPEALAVTERVCASVNVATTRAGI
                     NMDAVARMGEVVVATAKRTADRDGIGCAKLVVFCNAVEDNPFMAGAFHGIGEPDCVIN
                     VGVSGPGVVANAVEAEPDLDLGALASVIKRTAFKITRMGELVGRAASEKLGVPFGIVD
                     LSLAPTPAPGDSVARVLQAMGLERVGTHGTTAALAMLNDAVKKGGAMASSHVGGLSGA
                     FIPVSEDAIMIDAAADGAITFDKLEAMTCVCSVGLDMIAVPGDTPAATIAAIIADEAA
                     IGMINRKTTAVRLIPAPGKKVGDVVSFGGLLGRAPVIALHRESSEAFVARGGRIPAPL
                     QALNN"
     gene            complement(2852..3136)
                     /locus_tag="VFL84_04770"
     CDS             complement(2852..3136)
                     /locus_tag="VFL84_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET7607966.1"
                     /translation="MPTKSNRIIVTVIGVDTVGIIAQVSAVLAGANANIVDISQTTLR
                     EFFTMIMMADLTKATVPFDELKRRLNAKGEELGLKIDAQHEDVFKFMHRI"
     gene            3156..3950
                     /locus_tag="VFL84_04775"
     CDS             3156..3950
                     /locus_tag="VFL84_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET7607967.1"
                     /translation="MQQSSHRSFLAAAAFLLASSLAIAQPESPATIVPAPRTDENSRA
                     AHAELVRKAGTGVIDIYFVGDSITRRWGALDYPELLASFRDNFFGWNAANFGWGGDRT
                     QNILWRLDNGELPRAGPKVFVVQAGTNNLADFASADERVAAIAAGIEAIVERCRRHAP
                     EALVVLTGAFPRRDRPEFNSSIAAINAKLAAFGARQGVRYLDISERLVDARGALSPQM
                     SDDGLHPSLAAYQIWADALKPILVERLGAALDVDVAPAPTGNPAAR"
     gene            complement(3954..4844)
                     /locus_tag="VFL84_04780"
     CDS             complement(3954..4844)
                     /locus_tag="VFL84_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607968.1"
                     /translation="MKTTTWLAGGLLAGMGATIAASASAQSNACDRECLRGTVTTFLY
                     ALLEHDASKIPVAPKVRVTEDAVEKPLANVGLLRTVTKLRGYRQDILDERAGMAGAAV
                     IVEEVGAPVLLVVRLKVVDQKIAEIETVATRSRAEGLIFNIDGLGTPSAEMSYAPRKE
                     QLGSRQQMIDAAMHYPTGLNAAKTFAAVNAPFAPDAYRYENGQVMAGPNCTFAPGCEN
                     ISTQSLAIFERLGDVATRVIGVDERMGIVWLRMAWGVRQEGGEQLTVWEAFKVYDGQI
                     HAVEAFMRILPFEQRSGGWE"
BASE COUNT          711 a         1660 c         1675 g          842 t
ORIGIN      
        1 agctcgggcg cgcgcgcgtg ctcgtcgtcg gcttgggcgg gctcggctgt cccgcggcgc
       61 tctacctcgc gaccagcggc gtcggcaagc tcgtgctgaa cgacttcgac cgcgtcgacg
      121 agacgaactt gccgcggcag atcctgtacg cgcccgagga cgtcggcgcg ctgaaggtcg
      181 aggcggcgaa gaggcggttg accgcgttga accccggcgt cgaggtcgtc gtgctgcccg
      241 agcgtctcga cggcgcggcg ctcgaagccg ccgtcggcga cgtcgacatc gttctcgacg
      301 gcaccgacaa cttcgcggtg cgcttcgccg tcaaccgcgc gtgcgtcgcg cggcgcgtgc
      361 cgctcgtctc gggtgccgcc gtgcgtgtcg aaggccagat cgcggtcttt ccaaacgacg
      421 atgacggccc ttgctacgcg tgcctgtacg acgacgagga cgaatggctc ggcgactgcc
      481 agggcaacgg cgtgctcgcg cccgtgcccg gcgtcatcgg cacgttgatg gccgaggaag
      541 cgttgaagct cgcgctgggc tgggactctt cgctgcggaa ccgcctattg ctgtgggacg
      601 cgaaacgcgg cgagtggcaa agcgtcgcgc tgaagcgcga tcccgactgc gtcgtatgcg
      661 gccgcggccg cgtcacgtga cgtaatcacg cgcgtctgaa gcgcgtgctc acgctactcg
      721 gagctcgccg cgcgcggcat tcgtagaact gctcacgcac cgagcggctt ccggtcggat
      781 cactctcatg cgcgcaccgc gagcgcgcgc gctgtgctcg cgcgcgcttc atcgcagtgc
      841 gtttcgcgcg gcgatgcgtg gcgcgcgtca cgcaaaacgg cgtagctgcg cggttcacgg
      901 cggctggcac gcttggtgct tagcgtgtca cgtgcgcgtc atgtcgcgcg cttagtgtgt
      961 gatcgaacgc gctggatcag ggggcggaag cgcgaggttc cgtaaacgtg tcacttgcca
     1021 aggaggctct catgaagact acatcccgct ccgttctcgc gctcgcgacg ctgctcgcgt
     1081 cgctcggctg gtccgcgacg gcgctcgccg caccgactcg cgccggtgaa gtcgcgacga
     1141 aggtcgttcg cttcaaggat ctcgatctct cgacggccga aggcgcacag tcgctgtacg
     1201 agcgcatcaa gatggctgcg cgcatcgttt gccgcgaagc cccgcacgcg caagaacgcg
     1261 cgtgccgcac ccgcgccgtc gacgacgccg tgcgcatggt cggccggccg ctgctgagct
     1321 tggtccatcg ctcgatggtc gaccgcgtcg aggaggtggt gctgcggtga ttcccgcgag
     1381 ctcgggcgtg gccggttatt cccccgtgcc ggtcgcgccc gacgctcgcg attaccggcg
     1441 tctcgcgcgc tgacgagctc gtagtcagtt gttcagggct tgcagcggcg ccggaatgcg
     1501 cccgccgcgc gcgacgaacg cttcgctcga ttcgcgatgc agagcgatga cgggcgcgcg
     1561 gccgagcaag ccgccgaacg agacgacgtc gccgaccttc ttgccgggcg cggggatcag
     1621 gcgcacggcc gtcgtcttgc ggttgatcat gccgatcgcg gcttcgtcgg cgatgatcgc
     1681 cgcgatcgtc gcagcgggcg tgtcgccggg cacggcgatc atgtcgaggc cgacggagca
     1741 gacgcaggtc atcgcctcga gcttgtcgaa cgtgatcgcg ccgtcggccg cggcgtcgat
     1801 catgatcgcg tcctctgaaa cggggatgaa cgcgcccgag aggccgccga cgtggctcga
     1861 cgccatcgcg ccgcctttct tgacggcgtc gttcaacatc gcgagcgcgg ccgtggtgcc
     1921 atgcgtgccg acgcgctcga ggcccatcgc ctggagcacg cgcgcgacgc tgtcgccggg
     1981 cgccggcgtc ggcgccagcg agagatccac gatgccgaac ggcacgccga gcttttcgga
     2041 cgccgcgcgg ccgacgagct cgcccatgcg cgtgatcttg aacgccgtgc gcttgatgac
     2101 gctcgcgagc gcgccgaggt cgagatccgg ctcggcctcg acggcgttcg cgacgacgcc
     2161 gggtccgctg acgccgacgt tgatgacgca gtcgggctcg ccgatgccgt ggaacgcgcc
     2221 ggccatgaac ggattgtctt cgacggcatt gcagaacacg accagcttcg cgcagccgat
     2281 gccatcgcgg tcggcggtgc gcttcgcggt cgcgacgacg acctcgccca tgcgcgcgac
     2341 ggcgtccatg ttgatgccgg cgcgcgtcgt cgcgacgttc acggacgcgc agacgcgctc
     2401 ggtcacggcg agcgcctcgg ggatcgagga gatcagcgcg tcgtcgccgg gtgtcgtgcc
     2461 cttgtgaacc agagccgaga agccgccgag gaaattgacg ccgatctcgc gggccgcgcg
     2521 ctcgaggtgc tcggcgaaca gcacgtagtc gccgctcgcg gaggcttccg cgactaacgc
     2581 gacgggcgtc acggacacgc gcttgttgat gatcgggatg ccgtactcgc gctcgatctc
     2641 ctcgccgacg gcgacgagcc gcgcggccga ccggcagatc ttgtcgtacg acttcttcgc
     2701 cgccgctgcg aggctcgcgt ccgcgcagtt gcgcaagctg atgcccatcg tgatggtgcg
     2761 gatgtcgaga ttctccatct gcaccatgcg gatggtttcg aggatctcgt cgggcgagaa
     2821 ctggatggcc atgcgggcgg ctcggcgcgt gttagatgcg atgcatgaac ttgaacacgt
     2881 cctcgtgctg cgcgtcgatc ttgaggccga gctcttcacc cttggcgttg agcctgcgct
     2941 tcagctcgtc gaacggcacg gtcgctttcg taagatcggc catcatgatc atcgtgaaga
     3001 actcgcgcag cgtcgtctgc gagatgtcga cgatgttcgc gttggcgccg gccagcactg
     3061 ccgacacctg agcgatgata ccgacggtgt cgacgccgat gacggtcacg atgatgcggt
     3121 tcgatttggt cggcattggc cgtacactaa cctccatgca acagagcagc caccgttcgt
     3181 ttttggcggc tgcggcgttt ttgctggcga gcagcctcgc tatcgctcag cccgagtcgc
     3241 cggcgacgat agtacctgcg ccgcgcaccg acgagaactc gcgcgccgcg cacgccgagc
     3301 tcgtgcgcaa ggccggcacc ggcgtcatcg acatctattt cgtcggcgac tcgatcacgc
     3361 ggcgctgggg cgcgctcgat tatccggagc tgctcgcgag cttcagggac aacttcttcg
     3421 gctggaacgc cgcgaacttc ggctggggcg gcgaccgcac gcagaacata ctctggcggc
     3481 tcgacaacgg cgagctgccg cgcgccggcc ccaaggtctt cgtcgtgcag gccggcacga
     3541 acaatctcgc ggatttcgca tcggccgacg agcgcgtggc cgcgatcgcg gccggcatcg
     3601 aggcgatcgt cgagcgttgc cggcgtcatg cgcccgaggc gctcgtcgtg ctgaccggcg
     3661 cgttcccgcg gcgcgacagg ccggaattca actcgagcat cgcggccatc aacgcgaagc
     3721 tggcggcatt cggcgcgcgg cagggcgttc gctatctcga catctccgag cggctcgtcg
     3781 atgcgcgcgg agccttgagc ccgcaaatgt cggacgacgg cctgcacccg tcgctcgcgg
     3841 cttatcagat ctgggccgac gccttgaagc cgattctcgt cgaacggctc ggcgcagcgc
     3901 tcgacgtcga cgtcgcgccg gccccgaccg gcaatcccgc cgcccgctga agactactcc
     3961 cagccgcccg accgctgctc gaacggcagg atccgcatga acgcctcgac ggcgtggatc
     4021 tgcccgtcgt agaccttgaa cgcctcccag acggtcagct gctcgccgcc ctcttgccgc
     4081 acgccccacg ccatgcgcag ccagacgatg cccatccgct cgtcgacgcc gatcacgcgc
     4141 gtcgcgacgt cgccgaggcg ctcgaagatc gcgagcgatt gcgtcgagat gttctcgcag
     4201 cccggcgcga acgtgcagtt agggccggcc atgacttggc cgttctcgta gcggtacgcg
     4261 tcgggcgcga acggcgcgtt cacggccgcg aaggttttcg cggcgttcaa gccggtgggg
     4321 tagtgcatcg ccgcgtcgat catctgctgg cgcgagccga gttgctcctt gcgcggcgca
     4381 tagctcatct ccgcgctcgg cgtgccgagc ccgtcgatgt tgaagatcag gccttcggcg
     4441 cggctgcgcg tcgcgacggt ctcgatctcg gcgatcttct ggtcgacgac tttgagacgc
     4501 acgaccagca acacgggcgc gccgacttcc tcgacgatga cggccgcgcc ggccatgcct
     4561 gcgcgctcgt cgagaatgtc ctggcgatag ccgcgcagct tcgtgacggt gcgcagcagg
     4621 ccgacgttcg cgagcggctt ctcgacggcg tcttcggtga cgcgcacttt gggcgcgacc
     4681 ggtatcttgc tcgcgtcgtg ctcgagcagc gcgtagagga acgtcgtcac cgtgccgcgc
     4741 aggcactcgc ggtcgcaggc gttcgattgc gccgatgcgc tcgcagcgat cgtcgcgccc
     4801 atgcccgcca gcagaccgcc ggctaaccaa gtcgtcgtct tcattttttc tccggttgat
     4861 attcgtaggc cagctctttg cacgcgtc
//
LOCUS       DASSNN010000264         4105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_427506, whole genome shotgun sequence.
ACCESSION   DASSNN010000264 DASSNN010000000
VERSION     DASSNN010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427506"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1734)
                     /locus_tag="VFL84_04785"
     CDS             complement(<1..1734)
                     /locus_tag="VFL84_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011648349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7607969.1"
                     /translation="MGPIAALAQNAPQVIEIEQIVVTGTRVPDRSATETAVPVDVVSG
                     DAIANVGVTEINQALSVMLPSFNFPRPGLADGTDTIRPATLRGLSPDQTLVLMNSKRR
                     HASSLVNVNGTIGRGAAAVDLNTIPAAIVQSIEVLRDGASAQYGSDAIAGVINVRLKQ
                     DREGGDVMLAYGARDFDYDSPVSPPPAGATWPAPATIGSSGTDGEALTVSGWKGLGFG
                     DTGFLTLAAEYKDQEHTERGGYDFRQQYPLVGGAFDPREATFDRFNAWYGEPELEQLT
                     VFANGGGELDGGVYLYGWASYQDRESRSAGFFRRALDDRNVIQIYPDGFLPIIAPDVT
                     DLSAAAGATWDAGDWALDASLTFGSNEMQFGVEDTLNRSMGTASPTTFDAGGFKYDQL
                     VLNFSAVHAYDVGLASPLNVATGLEARQETYRIFAGEPASYINGGVLLPNGQPTASGS
                     QVFPGFRPANVTDADRTAVGAYVDFEANLTEKFLGSAAVRAEDYSDFGSNVSYKVAGR
                     YDFTDSFALRASAQTGFRAPSLQQQYFATTSTNFIAGVPFDITSFPVSDPIARALGAQ
                     DLDAEEAVNYSL"
     gene            complement(1875..2870)
                     /locus_tag="VFL84_04790"
     CDS             complement(1875..2870)
                     /locus_tag="VFL84_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7607970.1"
                     /translation="MLPTRREFLKTTAAGVATALAAGASRSMAQTAPASQPRMVKTDF
                     LEIGYEEHGDPAGTAVILLHGFPYDVRSFDGVVGPLAAAGHRVLVPWLRGYGPTRFLD
                     AAAPRMAEQAALGQDVVDFADALKIERFALGGWDWGNRAACIASILVPERVIGQVSIG
                     GYSVQNTVARGQPMQARQAAQFWYMWYLNTEQGAAALRANRHDFIRYLWDTWSPEWKY
                     TDEAYARSAPSFDNPDFVDIVVHSYRHRNMNAPGEPRFLEMERRLAEQPPVTVPAIVL
                     APGATGLGGRPSPNAAGDQRRFTSLKARRIVEGAGHDLPAQKPEAVSDALLELLA"
     gene            2935..3420
                     /locus_tag="VFL84_04795"
     CDS             2935..3420
                     /locus_tag="VFL84_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HET7607971.1"
                     /translation="MARFVAADASPLIGLATAGVLHLLRELYGIVTITRLVKDEIAGG
                     RRPGAQELDAAMRDGWLRVAPAPPETWALTGIDAGEASTIALAAEHDGALVLMDDALG
                     RAQAAAQGLELLDVAGVLLAAKRARLVRAVKPVVARLARRGFTIPTDTRLALLRDAGE
                     Q"
     gene            3455..>4105
                     /locus_tag="VFL84_04800"
     CDS             3455..>4105
                     /locus_tag="VFL84_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET7607972.1"
                     /translation="MEAPHPAADLTLSDADVEACIRVLRAIEADRSHLTRLGRELRRE
                     LLTFAGLVAKPERHDLVRMAKAFRRAEREAAKAHDRQLVDQALQRVQRRSPVYAPLKL
                     ERPAADDAGERAELENELACYVCKQRFTKVHRYYDSLCEPCGDFNYGKREQTADLAGH
                     YALVTGARVKIGYQAALKLLRSGAHVIVTTRFPRNAAMRYAGERDFAAWADRLEIVG"
BASE COUNT          620 a         1393 c         1471 g          621 t
ORIGIN      
        1 caacgaatag ttgacggcct cctcggcgtc gaggtcctga gcgccgagcg cgcgggcgat
       61 cgggtcgctg acggggaacg aggtgatgtc gaacggaact ccggcgatga agttcgtcga
      121 cgtcgtcgcg aagtactgtt gctgcagcga gggcgcgcgg aagcccgttt gcgccgacgc
      181 gcgcagcgca aacgaatcgg tgaagtcgta gcgcccggcg actttgtagg aaacgttgct
      241 gccgaaatcc gagtagtcct cggcgcgcac ggcggcggag cccaagaact tctccgtgag
      301 gtttgcttcg aagtcgacgt aggcgccgac ggccgtgcga tcggcgtcgg tgacgttcgc
      361 gggccgaaag cccgggaaga cttgcgagcc cgatgcggtc ggctgcccgt tcggcagcag
      421 cacgccgccg ttgatatacg aggccggctc gcctgcgaag atccgatagg tttcttggcg
      481 cgcttcgagg cccgtcgcga cgttgagcgg cgacgcgagg ccgacgtcgt acgcgtgcac
      541 ggccgagaag ttcagcacga gctggtcgta cttgaagccg cccgcgtcga acgtcgtcgg
      601 gctcgccgtg cccatcgagc gattcagtgt gtcctcgacg ccgaactgca tctcgttgct
      661 gccgaacgtc aacgacgcgt cgagtgccca gtcgccggcg tcccaggtcg cgccggccgc
      721 ggcgctcaag tccgtgacgt cgggcgcgat gatcggcagg aagccgtcgg gataaatctg
      781 gatgacgttg cggtcgtcga gcgcgcggcg gaagaacccg gccgagcgcg actcgcggtc
      841 ctgatagctc gcccagccgt acagatagac gccgccgtcg agctcgccgc cgccgttcgc
      901 gaacacggtc agctgctcga gctcgggctc gccgtaccag gcgttgaagc ggtcgaacgt
      961 cgcctcgcgc gggtcgaacg cgccgccgac gagcggatat tgctggcgga agtcgtaacc
     1021 gccgcgctcg gtgtgctcct gatctttgta ctcggcggcc agcgtcagga agcccgtgtc
     1081 gccgaagccg agccccttcc agccggacac cgtcagcgcc tcgccgtccg tgccgctgct
     1141 gccgatcgtc gccggcgcgg gccaggtcgc gccggccggc ggcgggctga cgggactgtc
     1201 gtaatcgaaa tcgcgcgcgc cgtaggcgag catgacgtcg ccgccttcgc ggtcctgctt
     1261 caaacgaacg ttgatgacgc ccgcgatcgc gtcggagccg tattgcgccg acgcgccgtc
     1321 gcgcaagacc tcgatcgatt gcacgatcgc tgcgggaatc gtgttcaagt cgacggccgc
     1381 ggcgccgcgg ccgatcgtgc cgttcacgtt gacgagcgac gacgcgtggc ggcgcttcga
     1441 gttcatgagc acgagcgtct ggtccggcga caggccgcgg agcgtggcgg ggcggatcgt
     1501 gtccgtgccg tcggcgaggc ccggccgcgg gaagttgaac gacggcagca tgaccgagag
     1561 cgcttggttg atctcggtca cgccgacgtt cgcgatcgcg tcgccgctga cgacgtcgac
     1621 gggcacggcc gtctccgtgg ccgagcggtc cggcacgcgc gtgcccgtca cgacgatctg
     1681 ctcgatctcg atgacttgcg gggcgttctg cgccaacgcg gcgatcggtc cgatcgcgcc
     1741 ggcgatcagc gcggtcgctc gtagcggcgg cggaatcctt gtctcgcgca cgggtcggct
     1801 cctcggttgt ccggaaggtg agcctagcga cgcgccgccg cgcgccgaat aggaccttcg
     1861 gtcctttgcg ggctttaggc gagcagctcg agcaacgcgt ccgagacggc ctccggtttc
     1921 tgtgcgggca agtcgtggcc cgcgccctcg acgatgcggc gcgctttcaa gctcgtgaag
     1981 cgccgctggt cgccagcggc gttcggcgag ggccggccgc cgaggcccgt cgcgcccggc
     2041 gcgagcacga tcgcgggcac cgtgacgggc ggctgctcgg cgagccgccg ctccatctcg
     2101 aggaaacgcg gctcgccggg cgcgttcatg ttgcggtggc gataggagtg cacgacgatg
     2161 tcgacgaagt cgggattgtc gaacgacggg gccgaacgcg cgtaggcctc gtcggtgtac
     2221 ttccactccg gcgaccacgt gtcccagaga taacgaatga agtcgtggcg attcgcgcgc
     2281 agcgcggcgg cgccttgctc cgtgttcaag taccacatgt accagaactg cgcggcctgc
     2341 cgcgcctgca tcggctggcc gcgcgccacc gtgttctgca cggaatagcc gccgatcgac
     2401 acctggccga tgacgcgctc gggcacgagg atcgacgcga tgcacgcggc gcgattcccc
     2461 caatcccagc cgccgagcgc gaagcgctct atcttcagcg cgtcggcgaa gtcgacgacg
     2521 tcttggccga gcgcggcttg ctcggccatt cgcggcgccg ccgcgtcgag aaagcgcgta
     2581 gggccgtagc cgcgcagcca cggcacgagc acgcggtggc ccgcggccgc gagcggcccg
     2641 acgacgccgt cgaacgagcg cacgtcgtaa ggaaagccgt gcagcaggat gaccgccgtg
     2701 cccgcggggt cgccgtgctc ctcgtagccg atctcgagga aatcggtctt gaccatgcgc
     2761 ggttgcgacg ccggcgcggt ttgcgccatc gagcggctcg cgcccgcggc gagcgcggtt
     2821 gcgacaccgg cggccgtcgt cttcaagaac tctcgtcgcg tcggcagcat gactcacccc
     2881 cttgctgatg gttgctacga tacgtcggcc ggcgaaatca gtcagcatgt gccaatggcc
     2941 agattcgtag ccgccgacgc gagcccgctg atcggcctcg ccaccgcggg cgtcctgcac
     3001 ctgctgcgcg agctctacgg catcgtcacg atcacgcggc tcgtcaagga cgagatcgcg
     3061 ggcgggcggc ggcccggcgc ccaagagctc gacgccgcga tgcgcgacgg ctggcttcgc
     3121 gtcgcgcccg cgccgcccga gacttgggcg ttgaccggca tcgacgccgg cgaggcgagc
     3181 acgatcgcac tggcggcgga gcacgacggc gcgctcgtgt tgatggacga cgcgctcggc
     3241 agagcgcagg cggccgcgca ggggctcgag ctgctcgacg ttgcgggcgt tctgctcgcg
     3301 gcgaagcgcg cgcggctcgt gcgcgccgtg aagcccgtcg tcgcgcggct cgcgcgccgc
     3361 ggcttcacga tccccacgga cacgcggctc gcgttgctgc gcgacgcggg agagcaatag
     3421 cgcgcggccg gtcggccggt acaatgcagc gcaaatggaa gcgccgcatc ccgccgccga
     3481 tctcacgctc tccgacgccg acgtcgaagc ttgcatccgc gtgctgcgcg cgatcgaagc
     3541 cgaccgctcg cacctgacgc gcttagggcg cgagctgcgc cgcgagctgt tgacgttcgc
     3601 gggcctcgtc gcaaaacccg aacggcacga tctggtccgc atggcgaagg cgttccggcg
     3661 ggccgagcgc gaagcggcga aggcccacga tcggcagctc gtcgatcagg cgctgcagag
     3721 agtgcagcgg cgctcgcccg tctacgcacc gctcaagctc gagcggccgg ccgcggacga
     3781 cgccggcgaa cgcgccgagc tcgagaacga gctcgcgtgc tacgtctgca agcaacggtt
     3841 cacgaaggtg caccgctact acgactcgtt gtgcgagccg tgcggcgact tcaactacgg
     3901 caagcgcgag cagaccgcgg atcttgcggg gcactacgcg ctcgtgacgg gcgcgcgcgt
     3961 gaagatcggc tatcaggcgg cgctcaagct gctgcgctcg ggcgcccatg tgatcgtcac
     4021 gacgcgcttt ccacgcaacg cggccatgcg gtacgcaggc gagcgcgact ttgcggcgtg
     4081 ggccgatcgc ctcgagatcg tcggc
//
LOCUS       DASSNN010000265         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_553945, whole genome shotgun sequence.
ACCESSION   DASSNN010000265 DASSNN010000000
VERSION     DASSNN010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553945"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VFL84_04805"
     CDS             <1..729
                     /locus_tag="VFL84_04805"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET7607973.1"
                     /translation="PLPGHPQIPHRFVACFAACDRRPLALEVTSCQVLAMSEPAVRLL
                     DDDEIVLRRTGVRFPVELRPTGFRADDPSTWPDAEGRLECVDGRLLYMPPCADVQQYV
                     AVDVAYVLRAWSEVSPEFVVGGNEAGMKLGRDIRAADAGVWRRDAAGPPTGRLHRTPP
                     VLAVEVAGEDEDEAVLRSKTQWYLRHGVSVVWIVLPEQRQVLVVGRAGETRCSRGERL
                     PLSAELPGLAPTVEQFFAQLDGRG"
     gene            complement(863..1720)
                     /locus_tag="VFL84_04810"
     CDS             complement(863..1720)
                     /locus_tag="VFL84_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7607974.1"
                     /translation="MAPDPNTRVPKYRPPPNACDAHCHIFGPGDKYPYAPDRSYTPPD
                     APLERFVELQKTLGLTRAVLVNASCHGVDNTVILDAIAQSGGRYRGVANVDESFSDRD
                     FAKLHEGGIRGIRFNFVQHLGGTPDMGEFKRLVARAAAFGWHVVLHFDAKDLLEFDSM
                     LRTLPVPFIIDHMGRVPTKDGLEQEPFKILLNVARMENCWVKICGAERISSKGPPFTD
                     AVPFAQAILKVAPDRALWGTDWPHPNIKKHMPNDGDLVDLIPLFMPDAELQQRVLVDN
                     PQRLYGFTN"
     gene            complement(1734..>2264)
                     /locus_tag="VFL84_04815"
     CDS             complement(1734..>2264)
                     /locus_tag="VFL84_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002542361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7607975.1"
                     /translation="GELKLQHLVVQTFFFRRKNINLEGKPRSWTDHLLWHHACHTVDL
                     FHYQTGETPSVVHALQGPLHPELKIAMDMSISMRTPSGAVCTLALSFNNDGPQGTFFR
                     YICDNGTYVARYDDLFDGKDQKIDLSGVAVSMNGIELQDREFVAAIRENREPKSSVAQ
                     VLPAMDTLDRLEKRLG"
BASE COUNT          376 a          713 c          767 g          408 t
ORIGIN      
        1 ccgctgccgg ggcacccaca aattccgcac agattcgttg cttgctttgc tgcctgcgat
       61 cgacgacctt tagccctgga agtcacatcc tgccaggtgc tagcgatgag cgaacccgcc
      121 gtgcggctcc tcgatgacga cgaaatcgtg ctgcgtagaa cgggcgttcg attcccggtg
      181 gagcttcgcc caaccgggtt tcgagctgac gacccgtcga cgtggcccga cgccgaggga
      241 cggctcgaat gcgtagacgg gagacttctg tacatgccgc catgcgcgga cgtccagcaa
      301 tacgtcgccg ttgacgtcgc atacgtgttg cgggcgtggt ccgaggtctc tccagagttt
      361 gtcgtcggcg gcaacgaggc cgggatgaag ctcggacggg acattcgcgc agccgatgcc
      421 ggcgtctggc gtcgcgacgc cgccgggccg ccaaccggcc ggctgcatcg cacaccgccg
      481 gtgctcgctg tagaagtcgc cggcgaagac gaagacgaag cagtgctgcg tagcaaaaca
      541 cagtggtacc taaggcacgg cgtctcggtc gtctggatcg tgttgccgga acagcgacaa
      601 gtgctcgtcg taggccgagc cggagagaca cgctgttcgc gcggcgagcg cttgccgttg
      661 tccgcagaac tgcccggatt ggcacccacg gtcgagcagt tcttcgcgca gctcgacggg
      721 cgcggctagc gccccgggcg ctgctgttgc gcggctgagc tcatccacgc cttgcgcgcg
      781 cagtagacct ccgccgaggg attctttgaa cgcgaagcgc ttgcatctcg aaaaacagag
      841 gatttgcgat cacgggcgag ggctaattcg taaagccgta gagccgctgc ggattgtcga
      901 cgagcacgcg ctgctgcagc tcggcgtccg gcatgaacag cggaatcagg tcgacgaggt
      961 cgccgtcgtt cggcatgtgc ttcttgatgt tcgggtgcgg ccagtccgtg ccccacagcg
     1021 cgcggtccgg cgcgactttc aggatcgcct gcgcgaacgg cacggcgtcc gtgaacggcg
     1081 ggcccttcga cgagatccgc tcggcgccgc agattttgac ccagcagttc tccatgcgcg
     1141 cgacgttgag caggatcttg aacggctcct gctcgagccc gtccttggtc ggcacgcggc
     1201 ccatgtggtc gatgatgaac ggcacgggca gcgtgcgcag catcgagtcg aactcgagca
     1261 ggtccttggc gtcgaagtgc agcacgacgt gccaaccgaa cgccgccgcg cgcgcgacga
     1321 gccgcttgaa ctcgcccatg tccggcgtgc cgcccaaatg ctgaacgaag ttgaagcgga
     1381 tgccgcggat gccgccttcg tgcagcttcg cgaagtcgcg gtcgctgaag ctctcgtcga
     1441 cgttcgcgac gccgcggtag cggccgccgc tctgcgcgat cgcgtcgagg atcaccgtgt
     1501 tgtcgacgcc gtggcaactc gcgttgacga gcaccgcgcg cgtgagcccc agcgtcttct
     1561 gaagctcgac gaaccgctcg agcggcgcgt cgggcggcgt gtagctgcgg tccggcgcgt
     1621 acgggtattt gtcgccgggc ccgaagatgt ggcagtgggc gtcgcacgcg ttgggcggcg
     1681 ggcggtactt cggcacccga gtgttcgggt ccggcgccat gcaaacgggg atctcagcca
     1741 aggcgcttct ccagccgatc cagtgtgtcc atcgcgggca ggacttgcgc cacgctcgac
     1801 ttcggctcgc ggttctcgcg gatcgccgcg acgaactcgc gatcttgcag ctcgatgccg
     1861 ttcatcgaca cggcaactcc cgacagatcg atcttctggt ccttgccgtc gaagagatcg
     1921 tcgtagcgcg caacgtaggt gccgttgtcg cagatgtagc ggaagaacgt gccctgtggg
     1981 ccatcgttgt tgaacgacag cgcgagcgtg cagaccgcgc ccgacggcgt gcgcatgctg
     2041 atgctcatat ccatcgcgat cttgagctcg ggatgcagcg gcccttgcag cgcgtgcacg
     2101 accgacggcg tctcgccggt ctggtagtgg aacaagtcga cggtgtgaca cgcgtggtgc
     2161 cacaacaggt gatcggtcca gctgcgcggc ttgccctcga ggttgatgtt cttgcggcgg
     2221 aaaaagaacg tttgcacgac gaggtgctga agcttgagct cgcc
//
LOCUS       DASSNN010000266         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1291509, whole genome shotgun sequence.
ACCESSION   DASSNN010000266 DASSNN010000000
VERSION     DASSNN010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291509"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..488
                     /locus_tag="VFL84_04820"
     CDS             69..488
                     /locus_tag="VFL84_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607976.1"
                     /translation="MQKKKSASRRQALSFDDVREIALSMPEVEEITAYGMPGFKAGKT
                     RFAVQPYPRPDVVPNSLGVPISFDERARVLAARPDVYYVTDHFLKYPGVLVRLASVDR
                     KELREILSAAWQYAMEHQVSAPRPRRKPSARAARARR"
     gene            complement(472..1572)
                     /locus_tag="VFL84_04825"
     CDS             complement(472..1572)
                     /locus_tag="VFL84_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7607977.1"
                     /translation="MRWVWKGLLGLALLLMLVVGSGLGYRAWRQHEGEAAMRIATPNG
                     VDDALFVEIGGAQQWVTIRGQDRANPVVLVLHGGPGSAIAGLATAFVPWERDFTVVQW
                     DQPGAGRTFRAAGRTIAPDLTIERMVDDGIELADWLRRRLGKDKIVLLGWSWGTILGV
                     QMAKARPDLFAAYVGTGQVVAMEEGEAIAYANVLAKARARNDAAALAELEAAGPPPYA
                     GLEEISTQRKWATTYEVGMPLEAAFVLPQLFAPRTQLADIYDLALATAASGTHFVGAE
                     MNGPMTRVDLRELGAELALPVFLIQGTADDFTPAELSRAWLDSLVAPQKVLVPIENAG
                     HFALTQHGEEFLRALRQHVRPLAAPESLSGAL"
     gene            complement(1595..2107)
                     /locus_tag="VFL84_04830"
     CDS             complement(1595..2107)
                     /locus_tag="VFL84_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7607978.1"
                     /translation="MRLRELAFGSIVSLVIAAASPVAADDADVQQLLDRAEIEELITR
                     YVTALDTRDADMYAGVFAEDAVYELPGNVVHHGREAIRKIVTDLQESRARNDAAGKPS
                     PSLYHAMFNHSIELVDATHAQHQCYAQTVRAAEGGQFVVGFMGRYEDYLVKLDGRWYI
                     QSRKLVSFVQ"
     gene            complement(2119..>3120)
                     /locus_tag="VFL84_04835"
     CDS             complement(2119..>3120)
                     /locus_tag="VFL84_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7607979.1"
                     /translation="HMCHLGGGYGRNGNGPQAEQALMLANANRGTPIHLLWTREEDFI
                     GTTYRAMGMARLKAALDADGWPLALEVRTGMQQDGFGPDASFDVASRYYVPNYRFSNH
                     TTNFHVPVGTRRGVGQAAHEFYRESFIDELAHAAGKDPYRYRRELIARTQLPFKADMI
                     KALDMAAEMSGWGTPLPKGTVRSIALEERGVETGNSATISAMVHTVSVSRDGRVKLER
                     VDVAHESGFGLVNPLSVKKQIEGQITWFYNDAMHQACNIENGAIAENNFNTFSMARIA
                     EDPLTINIQFFPTNHWLVGMGHDRGTSVQAAIANAIFEITGKRFRDLPLRQHDLSWR"
BASE COUNT          509 a         1056 c         1034 g          521 t
ORIGIN      
        1 aggatctcgc ggcgctcgcc gcgctcggct cgcgcaagcg gcactgaaga ggcccgaggg
       61 acaccaacat gcagaaaaag aagagcgcgt cccggcgtca agcgctgagc ttcgatgacg
      121 tgcgcgagat cgcgttgtcg atgcccgaag tcgaagagat cacggcgtac ggcatgcccg
      181 gattcaaggc cggcaagacc cgcttcgcgg tgcaacctta cccgcgcccc gacgtggtgc
      241 cgaactcgct cggcgtgccg atcagcttcg acgagcgcgc gcgcgtgctc gcggcgcggc
      301 cggacgtcta ctacgtgacc gatcacttcc tgaagtatcc gggcgtgctc gtgcgcctcg
      361 cgagcgtcga tcgcaaggag ctccgcgaga tcttgagcgc ggcgtggcag tacgcgatgg
      421 agcaccaagt atcggcgccg cggccgcgcc gcaaaccgag cgcgcgagcg gctagagcgc
      481 gccgctgaga ctttccggcg ccgccagcgg gcgcacgtgc tggcgcagcg ctcgcagaaa
      541 ttcctcgcca tgctgtgtca gcgcgaagtg tccggcgttc tcgatcggca cgagcacttt
      601 ttgcggcgct acaagcgagt cgagccaggc ccggctgagc tcggccggcg tgaagtcgtc
      661 ggccgtgcct tggataagga acacaggcaa cgcgagctcc gcgccgagct cgcgcaagtc
      721 gacgcgcgtc atcgggccgt tcatctcggc gccgacgaag tgcgttcccg aagcggccgt
      781 ggcaagcgcg aggtcataga tgtcggcgag ctgcgtgcgc ggcgcgaaga gctgcggcag
      841 aacgaaggcc gcctcgagcg gcatcccgac ttcgtacgtc gtcgcccact tgcgctgcgt
      901 cgagatttcc tcgaggcccg cgtacggcgg cgggcccgcc gcctcgagct ccgcgagggc
      961 ggcggcgtcg ttgcgcgccc gcgccttcgc gagcacgttc gcataagcga tcgcttcgcc
     1021 ctcttccatg gcgacgactt ggccggtgcc cacataggcc gcgaacagat cggggcgcgc
     1081 cttcgccatc tgcacgccga ggatcgtgcc ccacgaccag ccgagcagaa cgatcttgtc
     1141 cttgccgagc cgacgccgca gccagtccgc gagctcgatg ccgtcgtcga ccatacgctc
     1201 gatcgtgagg tcgggcgcta tggtgcgtcc cgcggcacgg aacgttctac cggcgcccgg
     1261 ctggtcccac tgcacgacgg tgaagtcgcg ctcccaggga acgaacgccg tcgccaagcc
     1321 tgcgatcgcc gagccgggac cgccgtgcag cacgaggacg acgggattcg cgcgatcctg
     1381 cccgcggatc gtgacccatt gctgcgcgcc gccgatctcg acgaacaacg cgtcgtcgac
     1441 gccgttcggc gtggcgatgc gcatcgccgc ttcgccctcg tgttggcgcc acgcgcgata
     1501 accgagaccg ctgcccacga cgagcatgag cagcagggcg aggccgagaa ggcccttcca
     1561 tacccatctc acttcgttcg ctccggcgcg cgggctactg cacgaacgag acgagcttgc
     1621 gcgattggat gtaccagcgg ccatccagct tcacgagata atcctcgtaa cggcccatga
     1681 agccgacgac gaactgaccg ccctccgcgg cgcggaccgt ctgcgcgtag cactgatgtt
     1741 gcgcgtgcgt cgcgtcgacg agctcgatcg agtgattgaa catcgcgtga tacaagctcg
     1801 gcgacggctt gcccgcggcg tcgttgcgcg cgcggctctc ctgcaagtcc gtgacgatct
     1861 tgcggatcgc ctcgcggccg tggtgcacga cgttgccggg cagctcgtag acggcgtcct
     1921 cggcgaatac gcccgcatac atgtccgcgt cgcgtgtgtc gagcgccgtc acgtagcgcg
     1981 tgatgagctc ctcgatctcg gcgcggtcga gcagctgctg cacgtcggcg tcatcggccg
     2041 ccaccgggct cgccgctgca atgacaagag aaacgatgct cccgaacgcg agctcccgta
     2101 gacgcatggc tttttctcct aacgccaaga caggtcgtgc tggcgaagcg gcaggtcgcg
     2161 gaaccgctta ccggttattt cgaaaatggc gttcgcgatc gcggcctgca ccgaggtgcc
     2221 gcggtcgtga cccatgccga cgagccagtg attcgtcgga aagaattgga tgttgatcgt
     2281 gagcggatcc tcggcgatgc gcgccatcga gaacgtgttg aagttgttct cggcgatcgc
     2341 gccattctcg atgttgcagg cctggtgcat cgcgtcgtta tagaaccacg tgatctggcc
     2401 ttcgatctgc tttttgaccg acaacggatt caccaagccg aacccggact cgtgcgcgac
     2461 gtcgacgcgc tcgagcttga cgcggccgtc gcggctcacg gagaccgtgt gcaccatcgc
     2521 gctgatcgtg gccgagttgc ccgtctcgac gccgcgctcc tcgagcgcga tcgaccgcac
     2581 cgttcctttc ggcaacggcg tgccccagcc cgacatctcg gcggccatgt cgagcgcctt
     2641 gatcatgtcg gccttgaacg gcagctgcgt gcgcgcgatc agctcgcgcc gatagcgata
     2701 cgggtcctta cccgcggcgt gcgcgagctc gtcgatgaag ctctcgcggt agaactcgtg
     2761 cgcggcctgc cccacgccgc ggcgcgtgcc gaccggcacg tggaaattcg tcgtgtggtt
     2821 cgagaagcgg tagttcggca cgtaatagcg cgacgcgacg tcgaacgaag cgtcgggccc
     2881 gaagccgtcc tgctgcatgc cggtgcggac ctcgagcgcg agcggccagc cgtcggcgtc
     2941 gagcgcggcc ttcagtcgcg ccatgcccat cgcgcgatac gtcgtaccga tgaagtcttc
     3001 ctcgcgcgtc cagagcaaat gaatcggcgt gccgcggttc gcgttcgcga gcattagcgc
     3061 ctgctctgct tgcgggccgt tgccgttgcg accgtaaccg ccgccgagat ggcacatgtg
//
LOCUS       DASSNN010000267         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_51178, whole genome shotgun sequence.
ACCESSION   DASSNN010000267 DASSNN010000000
VERSION     DASSNN010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51178"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1869)
                     /locus_tag="VFL84_04840"
     CDS             complement(22..1869)
                     /locus_tag="VFL84_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607980.1"
                     /translation="MRNEHAVMRRCSFLIFAAVSAAFAATAVAQNEVAGAWDQAGGGI
                     FGFQEEFVDRGGGPDLGDYAGLPINDALRYKASLYSPSWLTVPEHQCLPHPGTYAYRS
                     PGGLSIVKEYDPVTQRLVAYRFYGSYGLARTVWMDGRPHPPANARHTYEGFSTGHWEG
                     NRLAVETSHLKAGFLRRNGIAHSDRARMLEYFVRHDNYLTLVTAVDDPVYLDEPFVRS
                     TDFRVDPRPNTRLAEFGGFANGGDGEGFGASDVFYKCVPTEEIAIERGRVPSFMPGAN
                     ENIDMFSKRHNIPLDAALGGSATLYPEYTARLKPGSAAPAPERSAARPPRAPLPADAE
                     AATSLHVAGQVWLLTIGGHNVAVQVGNEGVLVVNPGPEEIADAVSAEIAKLAPGKRVR
                     IIVDTDDTLAHIGGNAKLASGTTPREQRAAVFAHENASLRIAAAGLSDRHSPTDTFFR
                     GTRELYFNDEPIEIIHVPAATTDGDVLVYFRKSDVVVTGSVIEDLTYPIIRADEGGSV
                     KGTLEALNHILDLTIAEYRAQGGTMVIPNRGRIYDEGDVAEYRDMLTIIRDRVQDAID
                     KGSTLAQVQAARLARDYDGRYAAATGPASANELIATVYRSLTPTAQNAR"
     gene            complement(1874..>2599)
                     /locus_tag="VFL84_04845"
     CDS             complement(1874..>2599)
                     /locus_tag="VFL84_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607981.1"
                     /translation="YASVPLNPAGRRVADGWDPAADAAAGEACRAFGAGGIMRLPTRL
                     KISWADDNTLRMETDAGSQVRTFHFGRAGAAEGEPSWQGYSVAEWLGIPPQGNPFGAL
                     VAPSQEASAARGAAAAGAPAGARGAPAGAGGAPAGRAVPPAPPPTRGSLKVVTTNLRP
                     GYLRKNGVPYSDHAVVTEYYDRFSMFGNDYLQVETVVTDPTYLTTPFVVSNQFKREPD
                     DSKWNPTPCATDAPLGKLQAPAF"
BASE COUNT          369 a          865 c          918 g          447 t
ORIGIN      
        1 gcgcttcgtt ggcgtgcgcc attatcgagc gttctgcgcg gtcggcgtca agctccggta
       61 gacagtggca atgagctcgt tggcggacgc gggtccggtg gcggccgcat agcggccgtc
      121 ataatcgcgc gcgaggcgcg cggcttggac ttgcgcgagc gtcgaaccct tgtcgatggc
      181 gtcttgcacg cgatcgcgga tgatcgtcag catgtcgcga tactccgcga cgtcgccttc
      241 gtcgtagatg cgcccgcggt tcgggatcac catcgtgccc ccctgcgcgc ggtactccgc
      301 gatcgtcaga tcgagaatgt ggttcagcgc ctcgagcgtg cccttgacgc tgccgccttc
      361 gtcggcccga atgatcgggt acgtcaggtc ctcgatgacg ctgcccgtca cgacgacgtc
      421 ggacttgcgg aagtagacga gcacgtcgcc gtcggtcgtc gccgcgggga cgtgaatgat
      481 ctcgatcggc tcgtcgttga aatagagctc gcgcgtgccg cggaagaacg tgtcggtcgg
      541 cgaatgccgg tcggagaggc ctgccgccgc gatgcgcagc gacgcgttct cgtgcgcgaa
      601 gaccgcggcg cgttgctccc tcggagtggt accgctcgcg agcttcgcgt tgccgccgat
      661 gtgcgcgagg gtgtcgtcgg tatcgacgat gatgcggacg cgcttcccgg gcgcgagctt
      721 cgcgatctcc gcgctgacgg catcggcgat ctcctcgggg ccagggttca cgacgagcac
      781 gccctcgttc cccacctgaa cggccacgtt gtggccgccg atcgtcagca gccacacttg
      841 gcccgcgacg tgcaacgacg tcgctgcctc cgcgtcggcc gggagcgggg cgcgcggcgg
      901 acgagcggcg cttcgttccg gcgcgggcgc ggcgctgccc ggtttcaggc gcgccgtgta
      961 ctcgggatac agcgtggcgc tgccgccgag cgccgcgtcg agcggtatgt tgtgacgctt
     1021 cgagaacatg tcgatgttct cgttcgcgcc gggcatgaac gacggcactc ggccgcgctc
     1081 gatcgcgatc tcctcggttg ggacgcactt gtagaacacg tcgctcgcac cgaacccctc
     1141 gccgtcgccg ccgttcgcga agccgccgaa ctcggcgagc cgcgtgttgg ggcgcgggtc
     1201 gacgcggaaa tccgtggagc gcacgaacgg ctcgtcgagg taaaccggat cgtcgacggc
     1261 cgtgacgagc gtcaggtaat tgtcgtggcg cacgaaatat tcgagcatgc gcgcgcggtc
     1321 gctgtgcgcg atgccgttgc gccgtaagaa accggccttc aagtgcgacg tctcgaccgc
     1381 gagccggttg ccttcccagt ggcccgtcga gaacccttcg taggtgtggc gcgcattcgc
     1441 cggcggatgc ggccggccgt ccatccacac ggtccgcgcg agaccgtagc ttccgtagaa
     1501 gcggtaggcc acgagccgct gcgtgacggg atcgtattcc ttgacgatcg aaagtccgcc
     1561 ggggcttctg tatgcgtagg tcccgggatg cggcaagcat tgatgctcgg gcaccgtcag
     1621 ccacgacggc gagtacagcg aggccttgta gcgcagcgcg tcgttgatcg gcagcccggc
     1681 gtagtcgccg agatcggggc cgccgccgcg atcgacgaac tcctcctgga agccgaagat
     1741 gccgccgccc gcttgatccc aggcgccggc gacttcgttc tgtgcgacgg ctgtcgcggc
     1801 gaacgccgcc gagaccgctg cgaagatcag gaagctgcaa cgacgcatca cggcgtgttc
     1861 gtttctcatg ggctcagaag gcgggcgctt gaagctttcc gagcggcgcg tccgtcgcgc
     1921 acggcgtcgg gttccacttc gagtcgtcgg gctcacgctt gaattgattg ctgacgacga
     1981 acggcgtcgt gaggtatgtc gggtcggtga cgacggtctc gacctgcagg tagtcgttgc
     2041 cgaacatcga gaaccggtcg tagtactccg tgacgacggc gtggtcgctg tacggcacgc
     2101 cgttcttgcg cagatacccg gggcgcaaat tcgtcgtgac gactttgagg ctgccgcgcg
     2161 tcggcggcgg tgccggcgga acggcacggc ccgccggtgc gccgcctgcc cccgccggtg
     2221 cgccgcgtgc tcccgccggt gcaccggctg cggctgcacc gcgcgcagcg gacgcctcct
     2281 ggctcggcgc gacgagcgcg ccgaacgggt tgccctgcgg cgggatgccg agccactcgg
     2341 ccaccgaata tccctgccag ctcggctcgc cctcggcagc ccctgcccgg ccgaaatgaa
     2401 acgtgcggac ctggctgccg gcgtcggttt ccatgcgcag cgtattgtcg tcggcccacg
     2461 agatcttgag gcgcgtcggc aaccgcatga tgccgccggc accgaaggcg cggcatgctt
     2521 cgccggctgc ggcgtcggcc gcgggatccc agccgtcggc gaccctgcgc ccggcggggt
     2581 tcaacggcac cgacgcgta
//
LOCUS       DASSNN010000268         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1291611, whole genome shotgun sequence.
ACCESSION   DASSNN010000268 DASSNN010000000
VERSION     DASSNN010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291611"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /locus_tag="VFL84_04850"
     CDS             complement(<1..1451)
                     /locus_tag="VFL84_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HET7607982.1"
                     /translation="MRHAWILAATAALALGTRAVADPRDAVDEAIRAHPSLTGSLVLE
                     HGEQALLARAWLVDHARRSIDVQYFIWSTDNIGILASEALLRAAQRGVAVRVIVDDLL
                     IDAPDETLLALAAHPNIDIRIYNPMHSVGVPWYRRLWGAVTDFRGSNQRMHDKVLIVD
                     GELAITGGRNMADEYFDYDHAYNFRDRDALVLGAVVASISASFERFWASELSVPVERL
                     LGEAAAAVDEAGTERVYRELAAYAADGTKFAPEVRAAIAAIPDDFSRLAAGIAWGDVD
                     FVSDMPGKNSGAHGLGGGGLSSAALGALVASAKSEVLIQSPYLVASDAAFEIFRQLHA
                     RGVRIRISTNSLASTDNLNAFSGYLNQRDELLAAGVEVFEYRPDPQIQAEIMDRYAAL
                     RAEAPVFALHAKTLVVDRRVVFVGTYNLDPRSENLNTEVGVVIHDAGQAAAVADAIAA
                     DMLPENSWSAARDDPDRHASWRKRTRAWLNSWLP"
     gene            complement(1511..>2030)
                     /locus_tag="VFL84_04855"
     CDS             complement(1511..>2030)
                     /locus_tag="VFL84_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7607983.1"
                     /translation="PRLKALRVPPVRALAPLVADACAAWTAGTAWDEVAVRLAAAAVR
                     FAGAPTRAPRSPRNAEHGVARAVRLIERDASRPLGLDVLAREASLSRFHFVRAFARLT
                     GLTPHRYVLRARLRRAAVRLATNGARIVDVAIESGFRDVSNFNHAFRAEFGTTPRAHR
                     QSAARRIVTPSA"
BASE COUNT          305 a          705 c          729 g          291 t
ORIGIN      
        1 ggcagccacg agttgagcca cgcgcgcgtg cgcttccgcc acgacgcgtg ccggtcggga
       61 tcgtcgcgcg ccgcgctcca gctgttctcg ggcagcatgt ccgccgcgat cgcgtcggcg
      121 acggcggcgg cttgccccgc gtcgtggatc acgacgccga cctcggtgtt taagttctcg
      181 gagcgcggat cgaggttgta ggtgccgacg aacacgacgc gccgatcgac gacgagcgtc
      241 ttcgcgtgca acgcgaacac gggcgcctcg gcgcgcagcg ccgcgtagcg atccatgatc
      301 tccgcttgaa tttgcggatc gggccggtac tcgaacacct cgactccggc ggcgagcagc
      361 tcgtcgcgtt ggttcaaata gccgctgaac gcgttcaggt tgtcggtcga cgccagcgag
      421 ttcgtgctga tgcggatacg cacgccgcgc gcgtgcagct gccggaagat ctcgaacgcc
      481 gcgtccgacg cgacgagata cggagactgg atcagcacct cgctctttgc gctcgcgacg
      541 agcgcgccga gcgcggccga gctcagtccc ccgccgccga ggccgtgcgc gccggagttc
      601 ttgcccggca tgtcgctgac gaagtcgacg tcgccccaag cgattcctgc cgcgaggcga
      661 gagaagtcgt ccgggattgc cgcgatcgcc gcgcgcactt cgggcgcgaa tttcgtgccg
      721 tccgcggcgt aggcggcgag ctcgcgatag acccgttccg tgccggcttc gtcgacggcg
      781 gccgctgcct cgccgagcaa gcgctcgacg ggcacggaga gctcgctcgc ccagaaccgc
      841 tcgaagctcg cgctgatcga cgcgacgacg gcgcccagca cgagcgcgtc gcggtcgcgg
      901 aagttgtatg cgtggtcgta gtcgaagtac tcgtcggcca tgttgcggcc gcccgtgatc
      961 gcgagctcgc cgtcgacgat cagcaccttg tcgtgcatgc gctgattcga gccgcggaag
     1021 tcggtgaccg cgccccacaa ccgccggtac cacggcacgc cgaccgagtg catgggattg
     1081 taaatgcgga tgtcgatatt cggatgcgcg gcgagcgcga gcagcgtctc gtcgggcgcg
     1141 tcgatgagca ggtcgtcgac gatcacgcgc acggcgacgc cgcgctgcgc ggcgcgcagc
     1201 agagcctcgg acgcgagtat tccgatgttg tccgtgctcc agatgaagta ctgaacgtcg
     1261 atgctgcgcc gcgcgtgatc gacgagccac gcgcgagcga gcagcgcctg ctcgccgtgc
     1321 tcgagaacca acgagccggt caggctcgga tgcgcgcgga tcgcttcgtc gacggcgtcg
     1381 cgcggatccg ctacggcgcg tgtgccgagc gctaaagcgg ccgtcgcggc gaggatccat
     1441 gcgtgacgca tcgcggcatc ttagagcatg ccgcggcgcc gaaacgtgcc gtgctaccgg
     1501 ctcgcgcccg tcaagcggaa ggcgtcacaa ttcgacgcgc tgccgactgc cgatgcgcgc
     1561 gcggtgtcgt gccgaactcc gcgcgaaagg cgtgattgaa gttggacacg tcgcggaagc
     1621 cgctctcgat cgcgacgtcg acgatgcgcg caccgttcgt ggccagccgg acggcggcac
     1681 ggcggagccg cgcgcgcagc acgtagcggt gcggcgtgag ccccgtgagg cgcgcgaacg
     1741 cgcgcacgaa gtgaaaccgg ctcaagctcg cctcgcgcgc gagcacgtcg agcccgaggg
     1801 gccggctcgc gtcgcgctcg atgagccgca ccgcccgcgc gacgccgtgc tcggcgttgc
     1861 gcgggctgcg cggcgcacgc gtcggcgcgc cggcgaagcg cacggccgct gccgccaacc
     1921 ggacggcgac ttcgtcccac gcggtcccgg ccgtccaggc cgcgcaagcg tcggccacga
     1981 gcggcgccag cgcgcggacc ggcggcacgc gcagcgcttt gagacgcggc
//
LOCUS       DASSNN010000269         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_792402, whole genome shotgun sequence.
ACCESSION   DASSNN010000269 DASSNN010000000
VERSION     DASSNN010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792402"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFL84_04860"
     CDS             <1..257
                     /locus_tag="VFL84_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HET7607984.1"
                     /translation="AAFRPWRLQALAFKALRTLEPKYPLWREFVYEYEHDRLAIDLLN
                     GSDLLRNWVDDPRSRPQDLDDLALADERAWRQERSKFLLY"
     gene            344..802
                     /locus_tag="VFL84_04865"
     CDS             344..802
                     /locus_tag="VFL84_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607985.1"
                     /translation="MKLKSILAAASLTLCGYAFAQDGEETIALLPEGAELPDVVTAAI
                     DLPKDANGEYRPSARAVESSAKGLATANAAREDGRAFGQSMAEQAKENRELRTRGEPP
                     DLAELLPDQVPDHVTLPTLPERPEPPQLPELPDHPVTPPVDTPATPPGRP"
     gene            complement(902..1843)
                     /locus_tag="VFL84_04870"
     CDS             complement(902..1843)
                     /locus_tag="VFL84_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016855514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7607986.1"
                     /translation="MQHPHAHTHDHSHDGHGHSQGHGHAANERSVGIAALLTGSFMLA
                     EIVGGLFAGSLALLADAGHMLTDFASLSLAWLGFRLTRQPADWRRTYGFDRFAVLVAF
                     VNGIALFAIAAWIVTEAAQRLSAPVDVRAWTMLWIAAAGLGVNVLSFLVLRSGDRDNL
                     NIRAAVLHVVGDLLGSVAAVVAAIVILTTGWTPIDPLLSIVVALIIVRSAWRVVADSG
                     HILLEGSPANVDSRALREHLRAALPFVLDVHHVHAWSISQERPMVTLHASIAASTDSA
                     AAVREIKRLLAAEFRITHATVEIEYDACGDGPHQPAC"
BASE COUNT          377 a          685 c          716 g          292 t
ORIGIN      
        1 acgcggcgtt ccggccgtgg cgcttgcaag cgctcgcatt caaggcgctg cgcacgctcg
       61 agccgaagta tccgctctgg cgcgagttcg tctacgagta cgagcacgac cggctcgcga
      121 tcgacttgtt gaacggcagc gacctcctgc gcaactgggt cgacgacccg cgcagccggc
      181 cgcaagacct cgacgacctc gcgctcgccg acgagcgcgc gtggcggcag gagcgcagca
      241 aattcctgct ctattgaagc cgcgccatat tttgttcaat ggaaccgaat gggcggttcg
      301 tacgtgtttg tggagcctgg aattcgcagg gaggaaacac acgatgaagc tgaaaagcat
      361 attggccgct gcgtcattga cgctctgcgg ctacgcgttc gcgcaggacg gagaggagac
      421 gatcgcgttg ttgcccgagg gcgccgagct gccggacgtc gtgaccgccg ccatcgatct
      481 gccgaaggac gccaacggcg agtaccgtcc gtccgctcgc gcggtcgaga gtagcgccaa
      541 gggactcgcc acggccaacg cggctcgcga agacgggcgt gcgttcggcc aaagcatggc
      601 cgagcaggcg aaggagaacc gagagttgcg gacccgcggc gagccacccg atctcgccga
      661 gctgttgccc gaccaggttc cggaccatgt cacgttgccg acgttgccgg agcggcccga
      721 gccgccgcag ctgcccgagc tgccggacca tcccgtcact ccgccggtcg atacaccggc
      781 gacgccgccg gggcggccat agcacaaacg cgacgctata cccgtcagga aggaagcccg
      841 cgggatctta tccgcgggct tttgttggac tcgcgacgga tccgagtggc ttaggcgcgg
      901 ctcagcaggc cggctggtgc ggtccgtcgc cgcaggcgtc gtactcgatc tcgaccgtgg
      961 cgtgcgtgat gcggaattcc gccgcgagca accgcttgat ctcgcgcacg gcggccgccg
     1021 aatccgtgct cgccgcgatg ctcgcgtgca gcgtgaccat cggccgctct tgactgatcg
     1081 accacgcatg cacgtggtgc acgtcgagca cgaacggcag cgcggcgcgc aagtgctcgc
     1141 gcagcgcccg cgagtccacg ttggccggcg agccttcgag caatatgtgg ccgctgtcgg
     1201 cgacgacacg ccaggccgag cgcacgatga tcagcgccac gacgatcgag agcagcggat
     1261 ctatcggcgt ccagccggtc gtgagaatca cgatcgcggc cacgacggcc gcgacggagc
     1321 ccaagaggtc gccgacgacg tgcagcaccg cggcacggat gttcaggttg tcgcggtccc
     1381 ctgagcgcag cacgagaaaa gacagcacgt tgacgcccaa gccggccgcc gcaatccaga
     1441 gcatcgtcca ggcgcgcacg tcgacgggcg cggagagccg ctgcgcggcc tcggtgacga
     1501 tccaggcggc gatcgcaaat agcgcgatgc cgttcacgaa cgccacgagc acggcgaacc
     1561 ggtcgaagcc gtaggtccgg cgccaatcgg cgggttgccg cgtgagcctg aaaccgagcc
     1621 aggcgagcga cagactggcg aagtcggtca gcatgtggcc cgcgtcggcg agcagcgcca
     1681 acgagcccgc gaacaggccg ccgacgatct cggcgagcat gaagctgccc gtcaggagcg
     1741 cggcgatgcc gacgctgcgc tcgttggcgg cgtggccgtg gccctgcgag tgaccatgcc
     1801 cgtcgtggga gtgatcgtgc gtgtgagcgt gggggtgctg cacgcggcgc atggtagcac
     1861 gcgcgaattg accgacccgc ccgtccacgg ggatcatggc gcccgggctt tcaagacaac
     1921 gacgagcgtc cggcgccacg ggcgccgaag aaattcgtgg tgctcaaggc acggcaaaaa
     1981 ctcggcaaat accgcatcga gcggcgtatc tccgaaggtc cgatcgcggc cgtctacgag
     2041 gccgtcgaca cgatccacgg gatgcacgtc
//
LOCUS       DASSNN010000270         5441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1291944, whole genome shotgun sequence.
ACCESSION   DASSNN010000270 DASSNN010000000
VERSION     DASSNN010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291944"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VFL84_04875"
     CDS             complement(<1..815)
                     /locus_tag="VFL84_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008389815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET7607987.1"
                     /translation="MAAVKKPRASAANTDYLVKDIGLADFGRKEIELAELEMPGLMST
                     RAEYGPKQPLKGARITGSLHMTIQTAVLIETLQALGAKVRWASCNVFSTQDHAAAAIA
                     ARGTPVFAYKGESLEEYWDYIDRTLDWGNSEGPNMILDDGGDATLFVHLGYEATKDPK
                     ILDKKPGSEEERILLAQLKKSLKRDPKRFKNMPSGIRGVSEETTTGVHRLYQMHKAGR
                     LLFPAINVNDSVTKSKFDNLYGCRHSLVDAINRATDVMLAGKIAVVAGYGDVG"
     gene            complement(826..1761)
                     /locus_tag="VFL84_04880"
     CDS             complement(826..1761)
                     /locus_tag="VFL84_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET7607988.1"
                     /translation="MSALICGSVAYDNIMVFEDSFKNHILPDKIHMLNVAFLVPNLRR
                     EFGGCATNIAYNLKMLGEEPLPMATVGQDYEPYRKRLKELGIKQTYIKQLDNTFTAQA
                     FITTDLSSNQIIAFHPGAMNFSEKNKVSDAKNVKIGMVSPDGRTGMVKHSEQFKKKKI
                     PFIFDPGQGMPMFNGDDLRKFIEEATWVTVNDYEWELLKDRTGWTEKDITSRVDALII
                     TLGGKGSEIHTKDKTYTIPAATPKSIQDPTGCGDAYRAGLLYGLLNKLDWETTGRIAS
                     LMGAIKIEHQGPQNHKFTLEQFKKRYKAAFGQKLS"
     gene            complement(1864..3015)
                     /gene="metK"
                     /locus_tag="VFL84_04885"
     CDS             complement(1864..3015)
                     /gene="metK"
                     /locus_tag="VFL84_04885"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET7607989.1"
                     /translation="MSKSYLFTSESVAEGHPDKIADQVSDAILDAILAQEPKSRVACE
                     TMTKTGMVIVAGEITTNAVIEVEHIVRKTVLEIGYDHSDKGFDGNTCAVLNGLGKQSQ
                     DIAQGVDRKSPEEQGAGDQGLMFGYATIETDSYMPAPIIYSHRLVEKQAEVRKNGKLP
                     WLRPDAKSQCTFRYENDKPVAIDAVVLSTQHAPEISLKDLQEAVMEEIIKPVLPAKWL
                     NKDTAYHINPTGRFVIGGPMGDCGLTGRKIIVDSYGGFARHGGGAFSGKDPSKVDRSA
                     AYAARYVAKNVVAAGLAERCEVQVSYAIGVAKPTSVTVETFGTNKVPEEKIIAAVNKN
                     FDLRPYGIIKMLDLLKPIYKRTASYGHFGREGFSWEKTDRAAALKADAK"
     gene            complement(3051..3959)
                     /locus_tag="VFL84_04890"
     CDS             complement(3051..3959)
                     /locus_tag="VFL84_04890"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET7607990.1"
                     /translation="MANDELKQQISAFLRDYSIEITPHDTGKLDAIRAELAAGTCVYV
                     AHPPGVPIDDIVTLAGQVQKLGLTATPHVIARKLESRDQLERALSKCQELGIRNALCV
                     AGDITAEKPAYNSSLEVLQTGLFKKYGFREVGIAGHPEGSKAIGEERVEQALRGKTEF
                     AKTAGFNVYFATQFGFDPQPFIDWEAETTAKGITLPIHVGMPGPASFKQLAKFAMLCG
                     VGASMRMLTSRTSAMANLLSTQAPDEMVTALARYKGSNPQSRFKKVHFFAFGGVVKTA
                     RWANAVISGRFVLNNQATGFRVEDVN"
     gene            complement(3989..5416)
                     /locus_tag="VFL84_04895"
     CDS             complement(3989..5416)
                     /locus_tag="VFL84_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607991.1"
                     /translation="MSQSSADSLLGEIEAFCRRTGIAESTFGRQAVNDGKLCVRLRNG
                     KDVTLETAAKIRGFIEGQPPAAALNGAVEIESNNKGTIMSANVKRKASSKAPAAANGA
                     ADRPFRFYDNRQKYLAFVNTTNEKWKVAERATRELALLKPTPPALRLFDAGMGDATVL
                     SHIMRAMHRRFPLMPFFIVGKEISLEDVRLSLEKLPDRFIEHPASVIVITNLYYAEAP
                     WLRPRDVVQAAALNWREVALEGASSYEYGEQLRALDSFLVDGWQVKASEKTGNPMYVR
                     PSVLVIYRKDHEFLLNNVIPKPGQVGGNYDLVIASQPWRARMSAEFKAKKVLGPLVRS
                     LGPSGRLLAVQSCGNDPALEMVHEVWPDEQPFKVNRHELIKVLRDELGREAKDFNFVS
                     GSDAKSIFRYEMHTLPSEVSQSIGTSTLFAAWNAASYVAQIEDERIESAMRDGDQYLQ
                     GTAKVLKKHKGLWFNDESFVVSRKQ"
BASE COUNT          859 a         1713 c         1726 g         1143 t
ORIGIN      
        1 ttaccgacgt cgccgtaacc cgcgacgacc gcgatcttgc cggcgagcat gacgtcggtc
       61 gcgcggttga tcgcgtcgac gagcgagtgg cggcagccgt acaggttgtc gaacttcgac
      121 ttcgtgaccg agtcgttgac gttgatcgcc gggaacagca accggcccgc cttgtgcatc
      181 tgatagaggc gatgcacgcc cgtcgtcgtc tcctcggaga cgccgcggat gccgctcggc
      241 atgttcttga agcgcttcgg atcgcgtttg agcgacttct tgagctgcgc gagcaggatg
      301 cgctcctcct ccgagcccgg cttcttgtcg aggatcttcg gatccttcgt ggcctcgtag
      361 cccaaatgca cgaacagcgt cgcgtcgccg ccgtcgtcca agatcatgtt cgggccttcg
      421 ctgttacccc agtcgagcgt gcggtcgatg tagtcccagt attcctcgag cgactcgccc
      481 ttgtacgcga acaccggcgt gccgcgcgcg gcgattgcgg cggccgcgtg gtcctgcgtc
      541 gagaagacgt tacagctcgc ccagcgcacc ttggcgccaa gcgcttgcag cgtctcgatc
      601 agcaccgccg tctggatcgt catgtgcagc gagcccgtga tgcgcgcccc tttgagcggc
      661 tgcttcgggc catactccgc gcgcgtcgac atgaggcccg gcatctcgag ctcggcgagc
      721 tcgatctcct tgcggccgaa atcggcgagg ccaatgtcct tgaccaagta gtcggtgttc
      781 gcggcgctcg cgcgcggctt tttgacggca gccatcgtga tctccttaac tcagtttctg
      841 gccgaaagcg gccttgtagc gtttcttgaa ctgttcgagc gtgaacttgt gattctgcgg
      901 tccctggtgc tcgatcttga tcgcgcccat gaggctcgcg atgcgccccg tggtttccca
      961 gtcgagcttg ttcagcaagc cgtagagcaa gccggcgcga tacgcatcac cgcagcccgt
     1021 cgggtcttga atcgatttcg gcgtcgcggc cggaatcgtg taggtcttgt ctttcgtgtg
     1081 gatctccgag cccttgccgc cgagcgtgat gatcagcgcg tcgacgcggc tcgtgatgtc
     1141 cttctccgtc caacccgtgc ggtccttcaa gagctcccac tcgtagtcgt tgacggtgac
     1201 ccacgtggcc tcttcgatga acttgcgcag gtcgtcgccg ttgaacatcg gcatgccctg
     1261 gcccggatcg aaaatgaacg ggatcttttt cttcttgaac tgctcggagt gcttgaccat
     1321 gccggtgcgg ccgtcgggcg acaccatgcc gatcttcacg ttcttggcgt cgctgacctt
     1381 gttcttctcg gagaagttca tggcgcccgg gtggaacgcg atgatctggt tcgaggacag
     1441 gtcagtcgtg atgaacgcct gcgccgtgaa cgtgttgtcg agctgcttga tgtaggtctg
     1501 cttgatgccg agctctttca agcgcttgcg gtacggctcg tagtcctggc cgaccgtcgc
     1561 catcggcagc ggctcctcgc cgagcatctt caagttatag gcgatgttcg tcgcgcagcc
     1621 gccgaactcg cgccgcaaat tcggcacgag gaacgcgacg ttcaacatgt ggatcttgtc
     1681 ggggaggatg tgattcttga acgaatcctc gaagaccatg atgttgtcgt aagcaaccga
     1741 cccgcaaatt aacgccgaca tcctttcctc ttcccttaaa tgacagtgaa cggcgaaagc
     1801 agaacgtaag gtgccggtgt tacggtttcg gttgaaccga accgtaacac cggcaccaat
     1861 ttcttacttg gcgtcggcct ttagggccgc agcgcgatcg gtcttctccc agctgaagcc
     1921 ctcgcggccg aagtggccgt aggacgccgt tcgcttgtag atcggcttca agagatcgag
     1981 catcttgatg atgccgtacg gccgcaggtc gaagttcttg ttgaccgcgg cgatgatctt
     2041 ctcctcgggc accttgttcg tgccgaaggt ctcgaccgtc acggacgtcg gcttcgcgac
     2101 gccgatcgcg tacgacactt gcacctcgca gcgctcggcg aggccggccg cgacgacgtt
     2161 cttcgcgacg tagcgcgcgg cataggcggc cgagcggtcc accttcgacg ggtccttgcc
     2221 cgagaacgcg ccgccgccgt ggcgcgcgaa gccgccgtag ctgtcgacga tgatcttgcg
     2281 gccggtcaga ccgcagtcgc ccatcggacc gccgatgacg aaccggcccg tcgggttgat
     2341 gtggtacgcc gtgtccttgt tcagccactt cgccggcagc accggcttga tgatctcttc
     2401 catgacggcc tcttggaggt ccttcagcga gatctccggc gcgtgctgcg tcgagaggac
     2461 gaccgcgtcg atcgcgacgg gcttgtcgtt ctcgtagcgg aacgtgcact ggctcttcgc
     2521 gtcggggcgc agccacggca gcttgccgtt cttgcgcact tcggcctgct tctcgaccaa
     2581 gcggtgcgag tagatgatcg gcgccggcat gtagctgtcg gtctcgatcg tcgcgtagcc
     2641 gaacatgagg ccctggtcgc cggcgccctg ctcttcgggg ctcttgcggt cgacgccttg
     2701 cgcgatgtcc tgcgactgct tgccgaggcc gttcagaacg gcgcaggtat tgccgtcgaa
     2761 gcccttgtcc gagtggtcgt aaccgatctc gagcacggtc ttgcgcacga tgtgctcgac
     2821 ttcgatgacg gcgttcgtgg tgatctcgcc ggcgacgatg accatgccgg tcttcgtcat
     2881 cgtctcgcag gccacgcggc tcttcggctc ttgcgcgagg atcgcgtcga gaatcgcgtc
     2941 cgagacttga tccgcgatct tgtccggatg cccttcggcc acggactccg acgtgaacag
     3001 atacgacttg ctcatggtta cctacctact cttctgtctg tcaaaaaaat tcagttgacg
     3061 tcttcaacgc ggaatccagt tgcctggttg ttcaagacga accgcccaga aatcacagca
     3121 ttggcccaac gcgcggtctt gacgacaccg ccgaaggcaa agaagtggac tttcttgaac
     3181 cgcgactgcg gatttgaacc cttgtagcgc gcgagcgccg tgaccatctc gtcgggcgcc
     3241 tgcgtgctca ggaggttggc catcgcgctc gtgcgcgacg taagcatacg catggaggcg
     3301 ccgacgccgc aaagcattgc gaatttcgcg agctgcttga aggaagccgg gcccggcata
     3361 ccgacgtgga tcggcagcgt gatgcctttg gccgtcgttt cggcctccca gtcgatgaac
     3421 ggctgcgggt cgaagccgaa ctgcgtcgcg aagtagacgt tgaagccggc cgttttcgcg
     3481 aactccgtct tgccgcgcag cgcctgctcg acgcgctcct cgccgatggc cttgctgcct
     3541 tcggggtggc ccgcgatgcc gacctcgcgg aaaccgtact tcttgaacag gcccgtctgt
     3601 agcacttcga ggctcgagtt gtaggccggc ttctcggccg tgatgtcgcc ggcgacgcag
     3661 agcgcgttgc ggatgccgag ctcctggcac ttgctaaggg cacgctcgag ctggtcgcgg
     3721 ctctcgagct tgcgcgcgat gacgtgcggc gtcgcggtca ggccgagctt ctgcacttgg
     3781 ccggccagcg tcacgatgtc gtcgatcggc acgccgggcg ggtgcgcgac gtagacgcag
     3841 gtgccggcgg cgagctccgc gcgaatggca tcgagcttgc cggtatcgtg cggcgtgatc
     3901 tcgatcgagt aatcccgaag gaaagccgag atctgctgct tcagctcgtc gtttgccatc
     3961 gtgctctcgt gacgttgagt ttcgggagct attgcttgcg cgacacgacg aagctttcgt
     4021 cgttgaacca aaggcctttg tgcttcttca ggaccttggc cgtgccctgt aggtactggt
     4081 cgccgtcgcg catggcgctc tcgatgcgct cgtcctcgat ctgcgcgaca tagctcgccg
     4141 cgttccaggc cgcgaacaac gtcgacgtgc cgatgctctg cgagacctcg ctcggcagcg
     4201 tgtgcatctc gtagcggaag atcgatttcg cgtcggagcc cgagacgaag ttgaagtctt
     4261 tcgcctcgcg tccgagctcg tcgcgcagca ccttgatgag ctcgtgccgg ttgaccttga
     4321 acggctgctc gtccggccag acctcgtgaa ccatctcgag ggccgggtcg ttgccgcagg
     4381 actgcacggc gagcagccgg ccggacggcc ctaggctgcg cacgagcggc ccgagcacct
     4441 ttttggcctt gaactccgcg ctcatgcgcg cgcgccacgg ctgcgacgcg atgacgagat
     4501 cgtagttgcc gccgacctgg cccggcttcg ggatcacgtt gttgagcagg aactcgtggt
     4561 ccttgcgata gatgacgagc acggacggcc gaacgtacat cgggttgccg gtcttctcgc
     4621 tcgccttgac ctgccagccg tcgaccaaga acgagtcgag cgcgcgaagc tgctcgccgt
     4681 actcgtacga cgacgcgcct tcgagcgcga cctcgcgcca gttcagcgcg gcggcctgca
     4741 cgacgtcgcg cggccgcagc cacggggctt cggcgtagta gagattcgtg atgacgatga
     4801 cgctggccgg atgctcgatg aaccgatcgg gcagcttctc gagcgagagg cgcacgtcct
     4861 cgaggctgat ctccttgccg acgatgaaga acggcatgag cgggaagcgc cggtgcatcg
     4921 cgcgcatgat gtgcgacagc accgtggcat cgcccatgcc ggcgtcgaac agccgcagcg
     4981 cgggcggcgt cggcttcagc agcgcgagct cgcgcgtcgc gcgctcggca accttccact
     5041 tctcgttcgt ggtgttgacg aacgccagat acttctggcg gttgtcgtag aaacgaaacg
     5101 gccggtcggc tgcgccgttg gcggctgcgg gagctttgga agaggctttg cgcttgacgt
     5161 tggcgctcat gatcgtgccc ttgttgttgc tctcgatttc gacggcgccg ttcaacgctg
     5221 cggcgggcgg ctgcccctcg atgaacccgc gaatcttggc cgcggtctcg agcgtgacgt
     5281 ccttgccgtt gcgcaacctg acgcagagct tgccgtcgtt gactgcttgc cgcccgaacg
     5341 tcgattcggc gatgccggtg cggcgacaaa acgcctcgat ttcgccaagc aaagaatcgg
     5401 ccgagctctg actcactggt tgcagtcccc cgaacgaatg g
//
LOCUS       DASSNN010000271         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_429356, whole genome shotgun sequence.
ACCESSION   DASSNN010000271 DASSNN010000000
VERSION     DASSNN010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429356"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..545
                     /locus_tag="VFL84_04900"
     CDS             123..545
                     /locus_tag="VFL84_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607992.1"
                     /translation="MLLRSAIQHHFARSARAERQNLRELFRIRRIGLLIGLAVLAAAF
                     GAGELVTSIGGNHLTRIVAESLVIGGWVAMWRPLEIFLYEWWPIRARARLYDRLSAVR
                     VEIVYAGIGGDPAASVAREMPATAPQSRAPAWRPMPSK"
     gene            complement(562..1785)
                     /locus_tag="VFL84_04905"
     CDS             complement(562..1785)
                     /locus_tag="VFL84_04905"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7607993.1"
                     /translation="MRTGSRATLIGYVALVAGLAAVGRAAAQQQVMSTSQGTLNVESL
                     ARLEFPWGIAALPDERFLITEKPGRLRVYSGGTLSPPIGGVPRVEYLEQGGLLDVAVD
                     PDFAQNQLVYLYFTERAERQPPNATLIPDPRLGPFVNKEDNVLKGGAVLRGRLDGNTL
                     RDARVIWRQEPKTIGMGHFGGRLVFGPDGKLFITSGERQRFEPAQDMGSNLGKVVRIN
                     PDGSIPDDNPFVRERGARGDIWTLGHRNPLGAAIDPTSNQLWVHEMGPAHGDELNLIE
                     PGKNYGWPIVSNGDHYDGTPIPKHATRPEFAPPVFYWYPAISPSGLHFYSGKMFDSWA
                     GNALIGGLSSQALVRVAFDGNKVASVEHICMGRRIRDVAEDSEGGVLLLTDAMDGELL
                     RLTPAARASASQGTC"
     gene            complement(1833..>2302)
                     /locus_tag="VFL84_04910"
     CDS             complement(1833..>2302)
                     /locus_tag="VFL84_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7607994.1"
                     /translation="ALEDETGGKVLEIRFARDANDEASFEAVVTADDELVYMRAYSGT
                     VPVEVLETAELPAWMAGWKLTAYMKSIEKAEVPLTAAILQAEEAENAPAIAAGLAKPL
                     SPTTAVLAYNIELIRGDQPVRVAIDAQTGRRIANPEALYETWTPTELVRRTLR"
BASE COUNT          384 a          765 c          733 g          420 t
ORIGIN      
        1 ggcggcgaag atcctgcctc gttcgccacc acgatcagtg ctcgagcaag gcagcgctag
       61 ctatagatag tactcgccgc gcgtcgaaaa gccgttgata tcgatcaacg cgtcaggagg
      121 cgatgttgct gcgctccgcg attcagcatc atttcgcgcg cagcgcgcgc gccgagcgac
      181 aaaatctccg cgagctcttt aggatccgcc gtatcggtct cttgatcggc ctcgcagtgc
      241 tcgctgcggc cttcggtgcc ggagagctcg tcacgagcat cggcggcaac catttgactc
      301 ggatcgtcgc ggaaagcttg gtgatcggcg ggtgggtagc gatgtggcgc ccgctggaaa
      361 tattcttgta cgaatggtgg ccgatccgcg cacgggcgcg cctgtacgat cggttgagcg
      421 cggtccgcgt cgagatcgtc tacgccggga tcggcggtga cccggcagca tccgtagccc
      481 gcgagatgcc ggcaacggcg ccgcaatcac gggcgccggc atggcggccg atgccgtcga
      541 aatagcggcc cgttcgcctc gtcagcacgt tccctggctc gcgctcgcgc gtgccgccgg
      601 tgtcagccgt aacagctcgc cgtccatcgc gtcggtcaag agcagcacgc ctccttcgga
      661 atcttcggcg acgtcgcgaa ttcgccgccc catgcaaatg tgctccacgc tcgcgacctt
      721 gtttccatcg aacgccactc ggaccaaagc ttgcgacgac aacccgccga tcaaggcatt
      781 accggcccac gagtcgaaca ttttgccgct atagaaatgc aggcccgacg gcgagatcgc
      841 cggataccaa tagaacacgg gcggcgcgaa ttccgggcgc gtcgcgtgct tcggaatggg
      901 cgtgccgtcg tagtggtcgc cgttgctcac gatcggccat ccatagttct tgccgggctc
      961 gatcaagttg agctcgtcgc cgtgcgccgg tcccatctca tggacccaaa gttgattgga
     1021 cgtcgggtcg atcgccgcac caagcggatt gcgatggccg agcgtccaga tatccccgcg
     1081 cgcgccccgc tcgcgcacga acgggttgtc gtcggggatc gagccgtccg gattgattcg
     1141 cacgaccttg ccgagattcg atcccatgtc ctgcgcgggc tcgaaacgct ggcgctcgcc
     1201 cgaggtgatg aagagcttgc cgtccggccc aaatacgagg cgaccgccga agtggcccat
     1261 gccgatggtc ttcggctcct gccgccagat gacgcgcgcg tcgcgcagcg tgttgccgtc
     1321 gagccgtccg cgcaggacag cgccgccctt gagcacgttg tcctccttgt tcacgaatgg
     1381 accgagacga ggatcgggaa tgagcgtggc gttcggcggc tgccgctcgg cgcgctcggt
     1441 gaaatagaga tagacgagct ggttctgcgc gaaatcgggg tcgacggcta cgtcgagcag
     1501 cccgccctgc tcgagatatt cgacgcgcgg gacaccgcca atcggcggcg acagggtgcc
     1561 gccggagtag acacgcaggc ggcccggctt ttctgtgatg agaaaccgct cgtccggaag
     1621 cgctgcaatt ccccacggaa actcgagccg cgcaagcgat tcgacgttca gcgttccctg
     1681 gctcgtcgac atcacttgct gctgcgcagc ggcgcggccg accgccgcta atcccgcaac
     1741 caacgcgacg taaccgatga gcgtggcacg acttccggtt ctcatcgcag cactcctttg
     1801 catggactgt tctttcgacg cgcaccggcg agtcagcgaa gggtgcggcg cacgagctct
     1861 gtcggcgtcc aagtctcgta gagcgcttcc ggattcgcga tgcgccgccc cgtctgcgcg
     1921 tcgatcgcga cgcgcaccgg ctggtcgccg cgaatgagct cgatgttgta tgcgagcacc
     1981 gcggtcgtcg ggctcaacgg ttttgcgagc cctgccgcga tcgccggcgc gttctcggcc
     2041 tcctcggcct ggaggatcgc cgccgttagc gggacctccg ctttctcgat gctcttcata
     2101 taggcggtca gcttccagcc ggccatccaa gccggcagct cagcagtctc caggacctcg
     2161 acggggaccg tgcccgaata ggcgcgcatg tagacgagct cgtcgtctgc cgtgaccacg
     2221 gcctcgaacg acgcctcgtc attggcatcg cgggcgaagc gaatctcgag cactttgccg
     2281 cccgtctcgt cctcgagagc ct
//
LOCUS       DASSNN010000272         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1292595, whole genome shotgun sequence.
ACCESSION   DASSNN010000272 DASSNN010000000
VERSION     DASSNN010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292595"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VFL84_04915"
     CDS             complement(<1..1317)
                     /locus_tag="VFL84_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7607995.1"
                     /translation="MSAVEQEVPGNVKVLKSVCRSCHGGCSTLMHVKDGVLTKVEGDP
                     EGPLNHGYLCPIGAATTQLVYHPDRLKYPQRRVGPKGSGKWKRISWDEALDEITEKVL
                     KIRAETGPESIAMGTGTGRHHIRWVSRFGNALGTPNWCEPGFAQCFHPRVNTCIQTMG
                     DLPVCDFTGGTPPKMVMYWGHNPPHSGPDGETRFNVRESLLSKPTVITIDPRETVVAK
                     KSDIWLQLRPGTDDALGLAFLNVIIEEDLYDHEFCEKYTHGFKELAEHVHKYTPEWAA
                     PITWVPADKIRAAARLFATVKPAMLEWGCAIEHTPKCIQTIRALSMIPAITGNIDVPG
                     GWIFGMKGAGRFPSLIENLDPEVEKKRLGYDKYKMLSSSEGADLPACHISSLIDAMYY
                     GEPYRVRAFLVFGNNTLTTYANTNLVYKALMKLDFMVCADLFMTPTA"
     gene            complement(1397..>2283)
                     /locus_tag="VFL84_04920"
     CDS             complement(1397..>2283)
                     /locus_tag="VFL84_04920"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7607996.1"
                     /translation="LGAHLAPDERPNLHIAEELWRSGKLPMRLRIQYDHRDIRDQSDI
                     HSATARIANTTVGLGDDRYKVVRFGEQVIQAGASDDEVYRVYQRLADAGWPLSQHTIR
                     EDEIERYIKIMERVAAQTPLADLRWSLEHVFEITPQQIARLKAIGVGVRVQDHDYIRN
                     PYSSWKAGPPFRTLLESGIKMGAGTDSGVVGALNPWLSLYYMVTGKDAGGDVIIPNEQ
                     ISRQAALRLYTADNAWYNFEEDLLGSIEPGKLADLVVLDRPYLTIPDEQIKEMRPVLT
                     MIGGRVVHARAPYDRLAN"
BASE COUNT          365 a          730 c          753 g          435 t
ORIGIN      
        1 ggccgtcggc gtcatgaaga gatcggcgca gaccatgaag tcgagcttca tgagagcctt
       61 gtagacgagg ttcgtgttcg cgtacgtcgt caacgtgttg ttgccgaaca cgaggaacgc
      121 gcgcacgcga tacggctcgc cgtagtacat cgcgtcgatc agcgacgaaa tgtggcacgc
      181 aggcagatcg gcgccttccg acgaagacag catcttgtac ttgtcgtagc cgagccgctt
      241 cttctcgacc tcggggtcga gattctcgat gaggctcggg aagcggccgg cccccttcat
      301 gccgaagatc cagccgccgg gcacgtcgat gttgcccgtg atggccggga tcatcgacag
      361 cgcgcggatc gtctgaatgc acttcggcgt gtgctcgatc gcgcagcccc actcgagcat
      421 cgcgggcttc acggtcgcga ataggcgcgc cgcggcgcga atcttgtctg cggggaccca
      481 cgtgatcggc gcggcccact ccggcgtgta tttgtggacg tgctcggcga gctccttgaa
      541 gccgtgcgtg tacttctcgc agaactcgtg atcgtagagg tcttcctcga tgatcacgtt
      601 caagaacgcg aggccgagcg cgtcgtcggt gccggggcga agctgcagcc agatgtcgga
      661 cttcttcgcg acgaccgtct cgcgcgggtc gatcgtgatg acagtcggct tgctcaacag
      721 cgactcgcgg acgttgaaac gcgtttcgcc gtccgggccc gagtgcggcg ggttgtggcc
      781 ccagtacatg accatcttgg gcggcgtgcc gcccgtgaag tcgcagacgg gcaaatcgcc
      841 catcgtctga atgcacgtgt tgacgcgcgg gtggaagcac tgcgcgaaac cgggctcgca
      901 ccagttcggc gtgccgagcg cgttgccgaa gcgcgatacc cagcggatgt gatggcggcc
      961 cgtgcccgtg cccatcgcga tcgactcggg ccccgtctcg gcgcggatct tgaggacctt
     1021 ctcggtgatc tcgtctagcg cctcgtccca cgagatgcgc ttccatttgc cggagccctt
     1081 cggcccgacg cggcgctgcg gatacttcag gcggtccggg tgatagacga gctgcgtcgt
     1141 ggccgcgccg atcggacaga gatagccgtg attcaacggg ccttcggggt cgccctcgac
     1201 cttcgtgagc acgccgtcct tgacgtgcat cagcgtgcta cagccgccgt ggcagctgcg
     1261 gcagacgctc ttcagaacct tgacgttgcc gggtacctct tgctctacag cgctcacgtc
     1321 cgttcccccg aacctcgcat gtattgctcc agccgagctt agcagaaggc acgcatgggg
     1381 tcccaatagg ttacgtctag ttcgcgagcc gatcgtacgg cgcgcgggcg tggaccacgc
     1441 ggccgccaat catcgtgagc acgggcctca tctctttgat ctgctcgtcc gggatcgtca
     1501 ggtacggccg gtcgagcacg acgaggtccg cgagcttgcc cggctcgatc gagcccagca
     1561 ggtcctcctc gaagttgtac caagcgttgt cggccgtgta gagcctaagc gcggcttggc
     1621 gcgagatctg ctcgttcgga atgatcacgt cgccgccggc gtccttgccc gtgaccatgt
     1681 aatagagaga gagccacgga ttcagcgcgc cgacgacacc ggaatccgtc ccagcgccca
     1741 tcttgatgcc gctttcgagc agcgtgcgaa acggcggtcc cgccttccag ctcgagtacg
     1801 ggttgcgaat gtagtcgtga tcctgcacgc gcacgccgac accgatcgcc ttgagccgcg
     1861 cgatttgctg cggcgtgatc tcgaacacgt gctcgaggct ccagcgcaaa tcggcgagcg
     1921 gagtctgcgc ggcgacgcgc tccatgatct tgatgtagcg ctcgatctcg tcctcgcgaa
     1981 tcgtgtgctg cgacagcggc cagccggcgt cggcgagccg ctggtagacc cgatacacct
     2041 cgtcgtccga cgcgccggct tggatcactt gctcgccgaa gcgcacgacc ttgtacctat
     2101 cgtcgccgag cccgacggtc gtgttcgcga tgcgcgccgt cgccgagtgg atgtcgctct
     2161 ggtcgcggat gtcgcggtgg tcgtactgga tgcgcaggcg catcggcagc ttgcccgagc
     2221 gccagagctc ctccgcgatg tggagattgg ggcgctcgtc gggcgcgagg tgcgcgccga
     2281 gga
//
LOCUS       DASSNN010000273         5850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_52466, whole genome shotgun sequence.
ACCESSION   DASSNN010000273 DASSNN010000000
VERSION     DASSNN010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52466"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="VFL84_04925"
     CDS             <1..1531
                     /locus_tag="VFL84_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7607997.1"
                     /translation="LARVTSRRKELAVRSALGAGNNRLVRMVLVESLLLALAGGVLGL
                     GVATGGLALVRALGLDRSSQGFAFVLDATALAYTLGAATLAAVVACLPPVLALLRDDL
                     TGAIREGGRQSGGGRRAQRLRGALVVAQLGMSVTLLVGAGLLTKNFYGLLEAGPGFNA
                     GSVWTARAALGGPRYADPESWPRFEHQAVEALRALPGVADAAFTSVLPFSPSNNQGSI
                     AIDGYVLPAGAAPPHAQHRSIDDRYLSVLGIPVVAGRSFTAHEAERVVIVDENLANKY
                     WPGGNAVGQRLRTDFGTPGEWFTIVGVVPAVKHSGLAEIPVKETVYWHYEQNPSSGVA
                     FALRTVVPPEQLARAVSATIAAIDPEVALYDSRTMDERVSSSLGPQRTPMVLTLVFAG
                     VAFALAVIGIYGVLAWGVAQRIGEIGVRMALGARAADIGRMILRQGGRLIVVGFVLGT
                     LGAVALGRVLSTRLFGVDSFDPTVLAITLVGLGAAAFVASWLPARRASRVDPMTALRE
                     E"
     gene            1538..3985
                     /locus_tag="VFL84_04930"
     CDS             1538..3985
                     /locus_tag="VFL84_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607998.1"
                     /translation="MRLDSWFRDLRYALRQLARAPGFTAVAALTLSIGIGANTAIFSV
                     VNHLLLRPPAHVVEPERVVSIWTSDFSGPSYGASSYPDYENFREQRDVMADVAAYGLV
                     PGNLVERDATVRLAIEQVTPNYFDVLGVRAALGRLLRGEADEGEAVVVLGHMLWRTRF
                     GADPAVVGRTVRINSGTFTVVGVAPEGFMGSQRIFGGVDAWVPLETLGGGRPGRLAER
                     GARGLMLLGRLQPGVSLDAARARFAVVANQLLASYPDEWRDVTNRGRAITVLPESESR
                     VPPEMRGAIVGFFALLLAGFALVLLICCVNVANLLLARAAGRVRDVAIRVSLGASRAR
                     LVRQLMIESVALAALGCAGAVLVTYAAVRLLSRWRPPGELELDVGIDWRVLAFALAAA
                     VVAAAIFGLAPALNLTRLGASAALKEGALALAGKRRFGLRGALITAQVSVSLVLLVCA
                     GLFLRSLQQAASVDLGFDPDNVVVASFDLRTQGYEEARGRAFYSEITDRLRGLPGVRA
                     VTLAQKVPLSGDSGRSRAVIEGYEPRAGEDMEFNNNVVGPEYFEVMRVPLVRGRGFSA
                     GDREGAPQVAVVNEAFAARYWPGEDPIGKRLSSFAGATSIEIVGVARDGKYQALTDAA
                     LPYIYRPFLQRYAEMTLHVRVARDANAFLPLLRSEIRAVDSQLPILRLATMRSETAFA
                     TWPQRIAATLLGGCAALALLLAAVGLYGVVAYAVSQRTREIGIRMALGAGRQAVVRMV
                     LEGSMKVVALGLAIGFGLSLAAGRAVESFLGEVSAADPIALIAGPLVMIACALVASWL
                     PARRAARIDPMKALREE"
     gene            3991..>5850
                     /locus_tag="VFL84_04935"
     CDS             3991..>5850
                     /locus_tag="VFL84_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7607999.1"
                     /translation="METFSKDIKHAWRMFRENKLFTATAIAALTLGIGVNIAIFSVVN
                     AVLLKPVPFPDPDTLVQVVNANNGVAGGPASSPAKYMHWRAQTDVLEAVAAARNQSLN
                     YGQGELLESIGAGQVSHEYFDVLRPPLVMGRWFTAEEDLPNAGYTVVLSFNFWTQHLN
                     SDPEILGKSITLSGNPYTVVGVVAREFDVRDLSAPPEVWVPFQLDPNTVDQGHFFTNV
                     ARLKPGVTLEQAQAKLLASASAFRDRFGQAAMPPTGGFSAITLQEALVRQARPTLLVA
                     LGAVGFVLLIACANVANLLLVRATGRRKELAVRTALGAGRWRIMRQLLTESLMLSLAG
                     GALGLLAGYAGMKWLLTVNTAGLPRLGQAGSLLGMDWRVVVFTVGLSIFTGILFGLIP
                     ALVASRADLNAVIKDSSSRSGSGFRQNKTRSVLVLVEVSLAVVLLVGAALLIRTSLAL
                     GGVDPGYTTENVLVMRTSLTGQRFQSAAVVDETARLALERIRGLPGVAAASMTCCVPL
                     QGALGLPFNIVGRTNEGPFTGGAGVHFAVAGYFDTFEIKVIRGRAFNESDTASSPPVI
                     IINEALAKQFWKDGADPLADQMLVAGGAMREFTGEPVRQIVGIVADVRAGSIAQ"
BASE COUNT          763 a         1985 c         2064 g         1038 t
ORIGIN      
        1 gctcgcgcgc gtgacgtcgc gccgcaagga gctcgcagtc cgctcggcgc tcggcgccgg
       61 aaacaaccga ctcgtgcgga tggtgctcgt cgagtcgctg ttgctcgcgc tcgccggcgg
      121 cgtgctcgga ctgggcgtcg cgacgggcgg gctcgcgctc gttcgcgcgc tcggcctcga
      181 tcgctcgagc caaggcttcg cattcgtgct cgacgccacg gcgctcgcct acacgcttgg
      241 cgcggcgacg ctcgcggccg tcgtcgcgtg tctgccgccg gtgctcgcgc tgctgcgcga
      301 cgacctcacg ggagcgatcc gcgagggcgg gcggcagagc ggcggcggcc gccgcgcaca
      361 gcggttacgc ggtgctctgg tcgtggcgca gctcggaatg agcgtcacgt tgctggttgg
      421 cgcgggcctt ctgacgaaga acttctatgg gctcctcgag gcgggccccg gattcaacgc
      481 cggcagcgtc tggaccgcgc gcgctgcgct cggcggaccg cgctacgccg acccggagtc
      541 ctggccgcgc ttcgagcatc aggcggtcga ggcgctgcgc gcgctgccgg gcgtcgccga
      601 tgcggcgttc acctcggtgc tgcctttctc gccttccaac aatcagggct cgatcgcgat
      661 cgacggttac gtgttgccgg ccggtgccgc tccgccgcac gcccagcatc gctcgatcga
      721 cgaccgctac ctctccgtgc tcggcattcc ggtcgttgct gggcgcagct tcactgcgca
      781 cgaggcggaa cgcgtcgtga tcgtcgacga gaatctcgcg aacaaatact ggcccggcgg
      841 taacgccgtc ggtcaacgat tgcgcaccga tttcggcacg cccggcgagt ggttcacgat
      901 cgtcggcgtc gtgccggccg tgaagcacag cggcctcgcc gagatacccg tgaaggagac
      961 cgtgtactgg cattatgagc aaaacccgtc atccggcgtc gcgttcgcgt tgcgcacggt
     1021 cgtgccaccg gagcagctcg cgcgcgccgt cagcgccacg atcgcggcga tcgatcccga
     1081 ggtggcgctc tacgactcgc ggacgatgga cgagcgcgtg tcttcgtcgc tgggcccgca
     1141 gcgtacgccg atggtgctga cgctcgtgtt cgccggcgtt gctttcgcgc tcgccgtgat
     1201 cggaatctac ggcgtgctgg cctggggcgt cgcgcagcgg atcggcgaga tcggcgtgcg
     1261 catggcgctc ggcgcgcgcg cggccgacat cggccgaatg atactgcggc aaggcgggcg
     1321 gctgatcgta gtcggcttcg tgctcggcac gctcggcgcc gtcgcgctcg gccgcgtgtt
     1381 gtcgacgcgg ttgttcggcg tcgactcgtt cgatcccacg gtgctcgcaa tcacgctcgt
     1441 gggcctcggc gccgcggcgt tcgtcgcgag ctggttgccg gcgcgccgcg cctcgcgcgt
     1501 cgatcccatg actgcgctgc gcgaggagta agcggcgatg aggctcgaca gctggtttcg
     1561 cgatctgcgc tatgcgctgc gccagctcgc tcgcgcgccg ggcttcacgg ccgttgcggc
     1621 gctcacgctc tcgattggca tcggcgccaa caccgcgatt ttcagcgtcg tgaatcacct
     1681 gctgctgcgt ccgccggccc atgtcgtcga gcccgagcgc gtcgtgtcga tctggaccag
     1741 cgacttcagc gggccgtcgt acggcgcgtc gtcgtatccc gactacgaaa atttccgcga
     1801 gcagcgcgac gtcatggccg acgtcgccgc gtacggcctc gtgcccggca acctcgtcga
     1861 gcgcgacgcg acggtgcggc tcgcgatcga gcaggtcacg ccgaactatt tcgacgtgct
     1921 cggcgttcgc gccgcgctgg gcaggctgtt gcgcggcgag gccgacgaag gcgaggcggt
     1981 cgtcgtgctc ggccatatgc tatggcggac gcgcttcggc gcggatcccg ccgtcgtcgg
     2041 ccgcacggtg cgcatcaaca gcggcacgtt cacggtcgtc ggcgtcgcgc ccgaaggatt
     2101 catgggtagt cagcgcatct tcggcggcgt cgacgcgtgg gtcccgctcg agacgctcgg
     2161 gggcgggcgc ccgggccgcc tcgccgagcg cggcgcccgc ggtctcatgt tgctcgggcg
     2221 gctgcagccc ggcgtctcgc tcgacgcggc gcgggcgcgc ttcgccgtcg tcgcgaatca
     2281 gctgctggct tcgtatccgg acgagtggcg cgacgtcacg aatcgcggcc gtgcgatcac
     2341 cgtccttccc gagagcgagt cccgcgtgcc gcccgagatg cgcggtgcca tcgtcgggtt
     2401 cttcgcgctg ctgctcgcgg gctttgcgct cgtgttgctg atctgctgcg tcaatgtcgc
     2461 gaacttgctg ctcgcgcgcg cggcgggccg cgtccgcgac gtcgcaatcc gcgtctcgct
     2521 cggcgcgtcg cgcgcgcggc tcgtgcggca gctgatgatc gagagcgtcg cgctggcggc
     2581 gctcggctgt gcgggcgccg tgctcgtcac ttacgcggct gtgcgtctgc tctcgcgctg
     2641 gcgcccgccc ggcgagctcg agctcgacgt cggcatcgac tggcgcgtgc tcgcgtttgc
     2701 gctggccgcc gcggtcgtcg ccgctgcgat cttcggtctc gcgccggctc tgaacctaac
     2761 ccggctcggc gcctcggcgg cgttgaagga aggcgcgctc gccctcgcgg gcaagcggcg
     2821 cttcggtttg cgcggcgcgc tgatcacggc gcaagtgtcg gtttcgttgg tgctgctcgt
     2881 ctgcgccggc ctgttcctgc gcagcttgca gcaggcggcg agcgtcgatc tgggtttcga
     2941 cccggacaat gtcgtcgtcg cttccttcga tcttcgaacg caaggctatg aggaagcgcg
     3001 cgggcgcgca ttctattcgg agatcacgga tcgcctgcgg ggcctgcccg gcgttcgcgc
     3061 cgtcacgctc gcacagaagg tgccgctgtc gggggacagc gggcggagcc gggccgtcat
     3121 cgagggctac gagccgcggg ccggcgagga catggaattc aacaacaacg tcgtcgggcc
     3181 cgagtacttc gaggtcatgc gcgtgccgct cgtgcgcggc cgcgggtttt cggccggcga
     3241 tcgcgaaggc gcgccgcagg tcgccgtcgt caacgaggcc ttcgccgcgc gttactggcc
     3301 cggcgaggat ccgatcggca agcgcctctc gtcgttcgcc ggcgccacgt cgatcgagat
     3361 cgtcggcgtc gcgcgcgacg gcaagtatca agcgctgacc gacgcggcgt taccgtacat
     3421 ctatcgtccc ttcctgcagc gctacgcgga aatgacgttg cacgtgcgtg tcgcacgcga
     3481 tgcgaatgcg ttcctgccgt tgctacgcag cgagatccgc gcggtcgata gccagctgcc
     3541 gatcttgagg cttgcgacca tgcgcagcga aacggcattc gcgacctggc cgcaacgcat
     3601 cgccgcgaca ttgctcggtg ggtgcgccgc gctcgcgctg ctgctcgcgg ccgtgggcct
     3661 ctacggcgtc gtcgcgtacg ccgtgagcca gcgcacgcgc gagatcggca ttcgtatggc
     3721 gctcggagcg ggccgccagg ccgtcgtgcg catggtgctc gaaggcagca tgaaggtcgt
     3781 cgcgctcggg ctcgcgatcg gtttcgggct gtcgctcgcc gcgggccgcg ccgtcgagtc
     3841 gttcctcggc gaagtgagcg cggcggaccc gatcgcgttg atcgcgggtc cgctcgtcat
     3901 gatcgcgtgc gcgctcgtcg cgagctggct gcctgcgcgc cgcgcggcgc gcatcgatcc
     3961 gatgaaagcg ttgcgtgagg agtagaacgc atggaaacct tcagtaaaga catcaagcac
     4021 gcgtggcgca tgttccgcga gaacaagctg ttcacggcga cggcgatcgc ggcgctgacg
     4081 ctcggcatcg gcgtcaacat cgcaatcttc tccgtcgtca acgccgtgct gctgaagccc
     4141 gtgccgttcc ccgatcccga cacgctcgtg caggtcgtga atgcgaacaa cggcgttgcg
     4201 ggagggcccg cgtcgtcgcc ggcaaagtac atgcactggc gcgcgcagac cgacgtgctc
     4261 gaggccgttg ccgcggcccg caaccaatcg ttgaactacg ggcagggcga gctgctcgag
     4321 tcgatcggcg cggggcaagt gagccacgaa tatttcgatg tgctgcggcc gccgctggtc
     4381 atgggccggt ggttcacggc cgaagaggat ctgccgaacg cgggctacac cgtcgtcttg
     4441 agcttcaact tctggacgca acacctcaac agcgatcctg agattcttgg caagagcatc
     4501 acgttgtcgg ggaatccgta caccgtggtc ggcgtcgtcg cgcgtgaatt cgacgtccgc
     4561 gatctcagcg cgccgccgga ggtctgggtg ccgtttcagc tcgatccgaa cacggtggat
     4621 caaggtcatt tcttcacgaa cgtggcgcgc ctgaagccgg gcgtcacgct cgagcaggcg
     4681 caagcaaagc tgctggcttc ggcgtcggct ttccgggacc gctttggcca ggccgcgatg
     4741 ccgccgaccg gcggcttcag cgcgatcacg ctgcaggagg cgctcgtgcg ccaagcgcgc
     4801 ccgactctgc tcgttgcgct cggtgccgtc ggcttcgttc tgctgatcgc gtgtgcgaac
     4861 gtcgcgaacc tgctcctggt ccgcgcgacg gggcgacgca aggagctcgc cgtgcggacc
     4921 gcgctcggtg ccgggcgctg gcgcatcatg cggcaactgc tgaccgagag cttgatgctg
     4981 tcgcttgcgg gcggcgcgct cggcctcttg gccggctatg ccggaatgaa atggctgttg
     5041 acggtcaaca cggcaggatt gccgcggctc gggcaggccg gctcgctgct cggcatggat
     5101 tggcgcgtcg tcgtcttcac ggtcggtctg tcgatcttca cgggaatcct gttcgggttg
     5161 atccccgcgc tcgtcgcctc gcgcgccgat ctcaacgccg tcatcaagga ctcgagcagc
     5221 cgctcgggca gcggcttccg tcagaacaag acgcgctcgg tgctcgtgct cgtcgaggtc
     5281 agcctcgccg tcgtgctgct cgtcggcgcc gcgctcctga ttcgcacctc actcgcgctc
     5341 ggcggcgtcg atcccgggta cacgaccgag aacgtgctcg tcatgcgcac gtcgctgacg
     5401 ggacagcggt tccagagcgc cgcggtcgtc gatgagacgg cgcgtttggc gctcgagcgc
     5461 atccgcggac tcccgggcgt cgcagccgcc tcgatgacgt gctgcgtgcc gctacagggc
     5521 gcgctcggct tgccgttcaa catcgtcggc cgcacgaacg aggggccgtt cacgggcggt
     5581 gccggcgtcc acttcgccgt cgcgggatac ttcgatacgt tcgagatcaa ggtgatccgc
     5641 ggccgcgcgt tcaacgagtc cgataccgcg tcgagtccgc ccgtcatcat catcaacgag
     5701 gcattggcga agcaattctg gaaggacggc gccgatccgc tcgcggatca gatgctcgtc
     5761 gccggcggcg ccatgaggga attcacgggc gagcccgtgc gccagatcgt cggcatcgtc
     5821 gccgacgtac gtgccggcag catcgcgcaa
//
LOCUS       DASSNN010000274         6124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_914182, whole genome shotgun sequence.
ACCESSION   DASSNN010000274 DASSNN010000000
VERSION     DASSNN010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914182"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFL84_04940"
     CDS             <1..346
                     /locus_tag="VFL84_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608000.1"
                     /translation="FESCSSGGMDLEAIGKEITDMKVAIGVIDHHSLQVETPTEVANH
                     IRMALKHIPVERLVLSSDCGMGREGMARRHAFYKMVSLVQGTNMVRKEHGIKEAECIA
                     ADPKLTLLDIKK"
     gene            complement(368..595)
                     /locus_tag="VFL84_04945"
     CDS             complement(368..595)
                     /locus_tag="VFL84_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608001.1"
                     /translation="MTAGAATLKTLGFGLSSAAAIFFASAGYGLLKMLLSGMSSGATI
                     GIAEFSTHSGLTIAFFVLAGLGAYVAKKCFW"
     gene            complement(592..873)
                     /locus_tag="VFL84_04950"
     CDS             complement(592..873)
                     /locus_tag="VFL84_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608002.1"
                     /translation="MKNQVGFVLGTLVLLGAASAASAAGSVATPRAGVETVEVVVVKA
                     KRVRPERIDVVVFAAKPPTVPVEARIPPAMPIEMPHLEFAVAATPAVRL"
     gene            complement(974..1996)
                     /locus_tag="VFL84_04955"
     CDS             complement(974..1996)
                     /locus_tag="VFL84_04955"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7608003.1"
                     /translation="MSRRLKFVASAFCALIPAVASAHHSRANYDMTKEIVVEGTVADL
                     AWKNPHISMTIERQGAGGEPSRLEIELTSVSEAMALGLPKEAIAPGSQVVVRAHPGRS
                     GPAAKAVGLDVRTGEGKVYPLNIDAKLAITPVAQPASGIAGHWAPTLESYPGLGVIAR
                     PMQVTEAGRAAAAEARRASAGTGGALPCEPFPPPFLSLFPDLRTIEVNGATVVMRFEG
                     AIGVPMERVVHLDQDAHPAGVTPSLMGHSIGRWEGETLVIDTVAFSAHRFGVLTFPSG
                     PNKHLVERLTLTKDRVQLEYTLTLEDPDVLAAPFSYTAIWNHRPDLEFSGVACDPEVS
                     RRAVQP"
     gene            complement(2034..3026)
                     /locus_tag="VFL84_04960"
     CDS             complement(2034..3026)
                     /locus_tag="VFL84_04960"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7608004.1"
                     /translation="MRSCGGALLVAAAWLISGAAQAHHSNAEYDRSALRELEGELVGV
                     AWANPHIAFKLRTVNDAGAAQEWELAGLPVALLEKAGLWGGMFAVGDRVKVAGYASRR
                     RAAMQVTNMLLPNGEEALFYPESRLRWSDKPAGGRFARESVSGNRRGLYRIWSVANLG
                     AYLATARAVTFNLTPQAQAKMAANPPTLDVCKPQGMPGFMLNPLPFGFVDRGDHIEMQ
                     HTSFGVVRTIQVTSARDPTTVALSDLGYSVGRWVGDTLEVRTTRIGWPYVDDDGRPQT
                     GGVEVLERFSLMNDGSLLRYTQTVTDPASLVEPMTVSWDLFDAGDEAIAPVQCQ"
     gene            complement(3019..3810)
                     /locus_tag="VFL84_04965"
     CDS             complement(3019..3810)
                     /locus_tag="VFL84_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET7608005.1"
                     /translation="MSTLTRREVVGVGAAAGVALALSQVAAQSRTGRRVTIDARPEPI
                     TIDTAATAVIVVDMQNDFGSKGGMFERAGIDISGIQKAVPPTARVLESARQAGITVVY
                     LKMAYQPDLSDLGAADSVNRVRHLQLGVGQHVTAPNGAASRVLVRDTWNSDIVDELKP
                     HADDIVLYKTRFSGFYKTDLDERLRALGVKHLLITGCTTSICVESTVRDAMFRDYLCV
                     LLTDCMSEPIGNQLARSNHEASLLSTEVLLGWVSESHQFLKSLHA"
     gene            complement(3899..4351)
                     /locus_tag="VFL84_04970"
     CDS             complement(3899..4351)
                     /locus_tag="VFL84_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HET7608006.1"
                     /translation="MRIWSLHPRYLDARGLVALWREALLAQAVLSGKTRGYRHHPQLL
                     RFRAHASPLGSIAEYLRVVHQEAARRDYHFASEKITRARVRGRLTVTRDQLEFEWRHL
                     LAKLKARGPGRLRELANVKRPRPHPLFRAVRGTIEQWEKATSPADKPS"
     gene            complement(4376..4627)
                     /locus_tag="VFL84_04975"
     CDS             complement(4376..4627)
                     /locus_tag="VFL84_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608007.1"
                     /translation="MDDKPTFWFPVKQYGWGWGLPVRWQGWVVFAAYLALMYAGIQYF
                     RPQRNVVGLLVYLVILTAVLIAIIVAKGERPAGWRWGKR"
     gene            complement(4643..5257)
                     /locus_tag="VFL84_04980"
     CDS             complement(4643..5257)
                     /locus_tag="VFL84_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HET7608008.1"
                     /translation="MGTSVAVKAFVFGVTIAATIGPIALLIINVSAVDGLARGIRASL
                     GAALADLVFATTAFAGGYAITAVLDAHRSELRAIASAVLVILGVWMAAQAWRSRSAGT
                     SSGKRWLRAPFLQTFALTIVNPLGVIAFMSLAVQVPVVTSLAAMAFLGFCVFAGSLLV
                     QLGLAVGGSLLARLTQHGGWLRALNLASGLGVAAFGVVGLVPNS"
     gene            complement(5264..5659)
                     /locus_tag="VFL84_04985"
     CDS             complement(5264..5659)
                     /locus_tag="VFL84_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7608009.1"
                     /translation="MRRGDKMPDRKAYPYATHLDIKFPALATVDVPALIKACTDRWYN
                     QTLCKVNDSVVRLGVMQGEYHWHRHENDDEFFFVLDGHFIIDLEDGSVDLQPRQGFVV
                     PRGVVHRTRAPDRAVILMVETASIVPTGD"
     gene            complement(5725..>6124)
                     /locus_tag="VFL84_04990"
     CDS             complement(5725..>6124)
                     /locus_tag="VFL84_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608010.1"
                     /translation="SDPPEVGSWVTGALALFFAICSLYFGWTLLYQVSGAPAPYPLPD
                     PALATLIACLGATAAAGLWLQRPWSRWVVYLISTLLIVCCAWIVWSFVHGGWPYKSGS
                     RSFALLLPLLLLLMFAVSTAVYVARLFRKR"
BASE COUNT         1202 a         2009 c         1890 g         1023 t
ORIGIN      
        1 gttcgagtcg tgcagctcgg gcggcatgga cctcgaggcg atcggcaaag agatcaccga
       61 catgaaggtc gcgatcggcg tcatcgatca ccactcgctg caggtcgaga ctccgacaga
      121 ggtcgcgaac cacattcgca tggcgctgaa gcacatcccg gtcgagcgcc tggtcctgtc
      181 gtccgactgc ggcatgggcc gcgagggcat ggctcgccgg cacgcgttct acaagatggt
      241 gtcgctcgtg caaggcacga acatggtccg caaggagcac ggcatcaagg aagccgagtg
      301 catcgccgcg gatccgaagc tcacgctgct cgacatcaag aagtaggtcg tcgtctactc
      361 gccgccgcta ccaaaagcac ttcttcgcca cgtaagcgcc gagcccagcg aggacgaaga
      421 acgcgatggt caggccgctg tgagtggaaa actccgcaat gccgatggtt gcaccgctgc
      481 tcatgcctga aagcagcatc ttgagcagcc cgtagccggc tgatgcaaag aatatcgctg
      541 cggccgatga cagaccgaag cccaacgtct tgagcgtcgc ggcgccggcg gtcacaaccg
      601 taccgccggc gtcgcagcca ccgcaaactc caaatgcggc atctcgatcg gcatcgcggg
      661 cgggattcga gcctcgacgg gcacggtcgg gggcttcgcc gcgaagacga cgacgtcgat
      721 ccgttccggc cggacgcgct tcgccttcac gacgaccact tcgaccgttt cgacaccggc
      781 tcgcggggta gcgacgctcc cggccgcgga ggccgcgctc gccgcgccca gcaacaccaa
      841 cgttccaagc acgaaaccca cctgattctt cacagcgctt ctccaaaatg agctgctcgt
      901 tacgctgaat agaaacgagc aagctccttt cggatgcagg gggcgcgcaa aaaaagaaga
      961 gagtgccgcg tggctacggc tgaactgctc gccgcgacac ttcggggtcg cacgcgacgc
     1021 ccgagaactc caagtcgggg cggtggttcc aaatcgccgt gtaagaaaac ggcgccgcaa
     1081 gaacgtccgg atcctcgagc gtcaacgtgt actcgagttg cacacgatct ttcgtgagcg
     1141 tgagccgctc gacgagatgc ttgttgggtc ccgatggaaa cgtgagcacg ccaaagcgat
     1201 gagcgctgaa tgcgacggtg tcgattacca gcgtctcgcc ttcccatcgt ccgatcgaat
     1261 ggcccatcag cgaaggcgtg acgccggcgg gatgagcgtc ctgatcgagg tgcacgacgc
     1321 gctccatcgg cacgccgatc gcgccctcga agcgcatcac gacggtcgcg ccgttgacct
     1381 cgatcgtgcg cagatcggga aagagcgaga gaaacggcgg cgggaacggc tcgcacggta
     1441 aggcgccgcc ggttccggca gaagcgcgcc tggcctcggc cgcggccgct cggcctgcct
     1501 cggtgacctg catcggcctc gcaatgacgc caaggcctgg gtaactctcg agggtcggtg
     1561 cccagtgccc ggcgattccg ctcgccggct gggcgacggg cgtaatcgcg agctttgcgt
     1621 cgatgttcag cggatagact ttgccttcgc cggttctgac gtcgaggccg acggccttcg
     1681 cggcggggcc gctccgtccc ggatgcgcgc gcacgaccac ctgcgagccc ggcgcgatcg
     1741 cctccttcgg caatcccagt gccatcgctt ccgagaccga cgtcagctcg atctcgaggc
     1801 gagacggctc gccgccggcg ccttgccgtt cgatcgtcat cgagatgtgc ggattcttcc
     1861 aagcgagatc ggcgaccgtg ccttcgacga cgatctcctt ggtcatgtcg taattcgcgc
     1921 ggctatggtg agcggaggcg acagcgggaa tcagcgcgca aaacgcggaa gcaacaaact
     1981 tcagccgtcg ggacatctgc tctccttcac ccgcccacgg tcatgatgga cgctcactgg
     2041 cactgcaccg gcgcgatcgc ctcgtcgccg gcgtcgaaca agtcccagct caccgtcatc
     2101 ggttcgacga gcgaggcggg atccgtgacg gtctgcgtgt agcgcagcag gctgccgtcg
     2161 ttcatcaacg agaatcgctc gagcacttct acgccgccgg tctgaggtct gccgtcgtca
     2221 tcgacgtatg gccatccgat ccgcgtcgtt cgaacctcga gcgtgtcgcc tacccatcgc
     2281 ccgaccgaat agccgagatc gctcaaagca accgtcgttg gatcgcgcgc gctcgtgact
     2341 tgaatcgtcc gcaccacgcc gaagctcgtg tgctgcatct cgatgtggtc gccgcggtcg
     2401 acgaagccga acggcaacgg attcaacatg aagcccggca tgccttgcgg cttgcagacg
     2461 tcgagcgtcg gcggattggc ggccatcttg gcttgcgctt gcggtgtcag gttgaaggtg
     2521 accgccctcg ccgtagccag gtaagcgccg agatttgcga cgctccagat ccggtacagg
     2581 ccgcgtcgat tgccgctgac cgactcgcgc gcgaagcggc cgcccgcggg tttgtcggac
     2641 cagcgaagcc ggctctcggg gtagaacaat gcttcctcgc cgttcggcag cagcatgttg
     2701 gtgacctgca tcgcggcgcg ccgtcgcgac gcgtagcccg ccactttcac gcgatcgcct
     2761 accgcgaaca tgccgcccca aaggccggcc ttctcgagca gagctacggg taagccggcg
     2821 agctcccact cttgcgctgc accggcgtcg ttcacggtgc gcagcttgaa tgcaatgtgc
     2881 gggttcgccc aagcgactcc gacgagctcg ccttccagct cgcgcaacgc gctcctgtcg
     2941 tactcggcat tcgaatgatg agcttgcgcg gcgcccgaga tcagccacgc cgctgcgaca
     3001 agcagcgctc cgccgcagct acgcatgcag cgacttcaaa aactgatgcg actctgacac
     3061 ccagccgagt agcacctccg tcgacagcag cgacgcctcg tggttgctgc gcgccaattg
     3121 gttgccgatc ggctcgctca tgcagtccgt gagcagcacg cagagatagt ccctgaacat
     3181 cgcgtcgcga acggtcgact cgacgcagat gctcgtcgtg catcccgtaa tgagaaggtg
     3241 tttcacgccc agagcgcgca aacgctcgtc gagatcggtc ttgtagaagc cgctgaaccg
     3301 cgtcttgtaa agcacgatat cgtcggcgtg cggcttgagc tcgtcgacga tgtcggagtt
     3361 ccacgtatca cgcaccagca cgcggctcgc agctccgttc ggcgcggtca catgttggcc
     3421 tacgcctagc tgcaagtgcc gaacccgatt gacggagtcg gccgcgccga ggtcggacag
     3481 gtcgggttgg tacgccattt tgaggtagac gaccgtgatg ccggcctgtc gcgccgactc
     3541 gagcacacgt gcggtcggcg gtactgcttt ttggatgccg gagatgtcga tcccggcgcg
     3601 ctcgaacatt ccgcccttgc tgccgaagtc gttctgcatg tcgacgacga tgacggccgt
     3661 ggccgctgta tcgatggtga tgggctcggg cctggcgtcg atcgtgacgc ggcgaccagt
     3721 ccggctctgc gcggccacct gacttagcgc caacgccacg cccgcggcgg ccccgacacc
     3781 cacgacttcg cggcgagtga gcgtgctcat tcggaacccc ctcggtagac cagcgcaacg
     3841 gcgcgaagct cgatgataag cctgaaagtc tagaccaggc gatatccggc ggaccggctt
     3901 acgacggttt gtcggccggc gacgtggcct tttcccattg ctctatggtg ccgcggacgg
     3961 cgcgaaatag tgggtgcggc cgcggcctct ttacattggc caactccctc agacgccccg
     4021 ggccacgcgc tttgagcttc gcgaggagat gccgccactc gaactcgagc tgatcacgcg
     4081 tgacggtgag ccgaccgcgc acgcgagcac gcgtgatctt ttcggaggcg aagtggtagt
     4141 cacgccgcgc cgcttcctga tggacgacgc gcaaatactc tgcgatcgaa ccgagcggcg
     4201 aagcgtgggc gcgaaaccga agcagttgcg ggtggtgtct gtatcccctc gtcttgccgc
     4261 tcaacacggc ttgcgcgaga agtgcttcac gccacaaggc caccagccct cgcgcgtcga
     4321 gataccttgg atggaggctc cagatgcgca cgatgccagc cgctgcgcgc cgatctcagc
     4381 gcttgcccca gcgccagccg gccggccgtt cgcctttggc gacgatgatg gcgatgagca
     4441 ccgccgtcaa aatcacgaga tacaccaaca gtccgaccac gttgcgctgc ggcctgaagt
     4501 attggattcc cgcgtacatc aacgcgaggt acgccgcgaa cacgacccaa ccttgccagc
     4561 gcaccggcaa gccccagccc cagccgtatt gcttcaccgg aaaccaaaac gtgggtttgt
     4621 cgtccatcac gatcactccg tgctaagagt ttggcacgag gccgacgacg ccaaaagccg
     4681 cgacgccaag accgctcgcg agattgagcg cccgcaacca gcctccgtgc tgcgtcagcc
     4741 gcgcgagcaa cgagccgccc accgccaggc cgagttgcac gagcaggctg ccggcgaaca
     4801 cacagaaacc caagaacgcc atggccgcca atgacgtgac gaccggcacc tgcacggcca
     4861 gactcatgaa cgcgatgact ccaagcggat tcacgatcgt caacgcgaag gtttgcagaa
     4921 atggcgcgcg cagccaccgc ttgcccgaag aagtgccggc gcttcgcgat cgccacgcct
     4981 gcgcggccat ccagacgccc agtatgacga ggactgccga tgcgattgct ctaagctcag
     5041 agcgatgcgc gtcgagcacg gcggtgatcg catagccacc cgcaaatgcg gtagtcgcga
     5101 agacgaggtc tgccagcgcg gcgccgagac ttgcccgaat accgcgagcc aagccatcga
     5161 ccgctgagac gttgatgatc aacagcgcga tcggcccgat ggtcgctgcg atcgtcacgc
     5221 cgaaaacgaa cgccttgact gcgacgctcg tgcccatggc gctttagtcg ccggtgggca
     5281 cgatggaagc cgtttccacc atcagaatga cggcgcggtc cggagcgcgc gtgcgatgaa
     5341 cgacacctct tggcacgaca aagccttgtc gtggctgcag atccaccgaa ccgtcctcga
     5401 gatcgatgat gaagtgacca tcgagcacga agaagaactc gtcatcgttc tcgtggcggt
     5461 gccagtgata ctcgccttgc atcacgccga ggcgaacaac cgaatcattc actttgcaca
     5521 aggtttggtt gtaccaacga tcggtgcacg ctttgatcag agcgggcaca tcgaccgtcg
     5581 ctagcgccgg gaacttgatg tcgaggtgtg tggcatacgg atacgctttc ctgtctggca
     5641 ttttgtctcc ccgtctcatg ttgccgctcg acgcccgggc tcaccgacaa gccacgaacc
     5701 gggcaacatt tcccggccat ccacctatcg ctttcgaaag agccttgcga cgtaaaccgc
     5761 tgttgacact gcgaacatca gtaagagcag cagcggaagc aacagcgcaa aggaccggct
     5821 gccgcttttg tagggccaac cgccgtggac aaacgaccaa acaatccagg cgcagcagac
     5881 gatcagcagc gtcgagatga gatagacaac ccatcgcgac cacggacgtt ggagccacag
     5941 cccagccgct gccgttgcgc cgaggcaagc gataagcgta gccaatgccg gatcgggaag
     6001 ggggtatgga gctggcgctc cgctaacctg atacaaaagc gtccagccga agtagaggga
     6061 acagatcgca aagaacaggg ccaacgctcc ggtcacccaa gagccgacct cgggcggatc
     6121 gctg
//
LOCUS       DASSNN010000275         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_285976, whole genome shotgun sequence.
ACCESSION   DASSNN010000275 DASSNN010000000
VERSION     DASSNN010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285976"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1150
                     /gene="galE"
                     /locus_tag="VFL84_04995"
     CDS             152..1150
                     /gene="galE"
                     /locus_tag="VFL84_04995"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET7608011.1"
                     /translation="MTQKTILVTGGAGYIGSHVVRQLGEAGEKIVVLDDLSTGFRQAV
                     LYGDLVVGNTGDAACLEKLFERYEIDTVMHFAAHTVVPESVANPLKYYGNNTCASRTL
                     LEHALRAGVKHVVFSSTAAVYGIPQDGEASEETPTAPINSYGTSKLMTEWMLRDVAAV
                     NPLRYVALRYFNVAGSDPGGRIGQATPRATLLTKVACEVVVGKRPYVAIFGTDYPTPD
                     GTGVRDYIHVEDLADAHLKALAYLRRGGESTTLNCGYGRGYSVRELLSVVERVAGHPI
                     AKREEPRRAGDPPTLVARATRIRATLGWQPRHDDLTKIVTTSLEWERKLAAGHWRA"
     gene            1176..>2411
                     /locus_tag="VFL84_05000"
     CDS             1176..>2411
                     /locus_tag="VFL84_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET7608012.1"
                     /translation="MKMRRPRTLLGMVLTGLFVVAVPLLVAVGNAMFKLGQLAAQSEV
                     VLADSRTAALQTERLTNLLISMERNALTYLALKGDPAAPNTLASYDQGQADFEKSVSE
                     IRVLPSDPPIRDQLARLSSISKDVHRMLRTGATGETIRERFRMLSEASRAVANGMRAT
                     TNARFEALQENTRSAQQQLLWLTAGLIPGIMILVGLFLLLVGRPLRQVDRAIRELGEG
                     DFSRPITVSGPHDIETLGRQLEWLRHRLKESTDEKNKFLRHMSHELKTPLANIREGTE
                     LLLDGSVGPLDVQQQEVTGILRDNGVKLQKLIENLLTFSAWQTHTAALELSEFELKPM
                     VFSVLSQHRLALSNKKIKLQLAIAAIIVLADEGKLKLVLENLLSNAIKFTPSGGSIHV
                     GAAMQGHDLVMDVSDDGPGV"
BASE COUNT          438 a          821 c          767 g          385 t
ORIGIN      
        1 cccgcgacct ggccggggaa ccgcctgtcg agccggagtc tccggtttgg acgcaggact
       61 ccggcagccc caaactgcga cccgattccg ggccgcacaa aggcgtcctg ctacactcat
      121 gggtcgagat tatgtcgccc catttaagtc tatgacgcaa aaaacaattc tggtgaccgg
      181 cggggccggc tacatcggct cgcacgtcgt gcgccagctc ggcgaagccg gcgagaagat
      241 cgtcgttctc gacgacctgt ccacgggttt tcggcaagcc gtgctctacg gcgatctcgt
      301 cgtcggcaac acgggcgacg ccgcttgcct cgagaagctg ttcgagcgct acgagatcga
      361 taccgtcatg cacttcgcgg cccacaccgt ggtgcccgag tccgtcgcga acccgctcaa
      421 gtattacggc aacaacacct gcgcgagccg cacgctgctc gagcacgcgc tgcgcgccgg
      481 cgtcaagcac gtcgtgttct cgtcgacggc ggccgtctac ggcattccgc aggacggcga
      541 agcgtccgag gagacgccca cggcgccgat caactcgtac ggcacctcga agctcatgac
      601 ggaatggatg ctgcgcgacg tcgccgccgt caacccgctg cgctacgtcg cgctgcgcta
      661 cttcaacgtc gccgggtccg atcccggcgg ccgcatcggc caggccacgc cgcgcgcgac
      721 gcttctcacg aaggtcgcgt gcgaggtcgt cgtcggcaag cggccttatg tcgccatctt
      781 cggcaccgac tacccgaccc cggacggcac cggcgtgcgc gactacatcc acgtcgagga
      841 tctggcggac gcgcacttga aagccctggc atacttgcgc cgcggcggcg aatccacgac
      901 attgaactgc ggttacgggc gcggttacag cgtccgcgag ctgctttccg tggtagagcg
      961 cgtcgctggg cacccgatag ccaaacggga ggaacctcgt agggccggcg atccgccgac
     1021 attggtcgct cgcgccactc gtattcgtgc aacgctcggc tggcagccgc ggcacgacga
     1081 cctcaccaaa atcgtaacaa cgtcgctcga atgggaacga aaactcgctg cggggcattg
     1141 gcgcgcttga tcgggaaaga ccgggggtct cgcgaatgaa gatgcggcgc ccgcgaacgc
     1201 tgctcggcat ggtgctgacc ggcctcttcg tcgtcgccgt gccgctgctc gtcgcggtcg
     1261 gcaacgccat gttcaagctc ggccagcttg cggcgcagag cgaggtcgtg ctcgccgaca
     1321 gccgcacggc cgcgctgcaa acggagcgcc tcacgaatct gctcatcagc atggagcgca
     1381 acgcgctcac ctacttggca ctcaaggggg acccggccgc gccgaacacg ctcgcgtcct
     1441 acgaccaggg ccaagccgac ttcgagaaaa gcgtctccga gattcgcgtg ttgccgagcg
     1501 atccgccgat ccgcgatcag cttgcgcggc tctcgtcgat ctcgaaggac gtgcacagaa
     1561 tgctccgcac cggcgcgacg ggcgagacga tccgcgagcg cttccgaatg ctgagcgaag
     1621 cgtcgcgcgc cgtcgcgaac ggcatgcgcg cgacgacaaa cgcgcggttc gaggcgctgc
     1681 aggagaacac gcgttccgcg cagcagcagc tcttgtggct gaccgccggg ctgatccccg
     1741 gcatcatgat cctcgtcggc ctattcttgc tgctcgtcgg ccgccccttg cggcaagtcg
     1801 accgcgcgat ccgcgagctc ggcgagggcg atttcagccg gccgatcacg gtcagcggcc
     1861 cgcacgacat cgagacgctc gggcgtcagc tcgagtggct gcgccatcgg ctcaaggaat
     1921 cgaccgacga gaagaacaaa ttcctgcgcc acatgtcgca cgagctcaag acgccgctcg
     1981 cgaacatccg cgaaggcacg gagctgctgc tcgacggctc cgtcggcccg ctcgacgttc
     2041 agcagcagga agtcacgggc atcctgcgcg acaacggcgt caagctgcag aagctgatcg
     2101 agaacctgct gacgttcagc gcctggcaga cgcacacggc ggcgctcgag ctctcggagt
     2161 tcgagctgaa gccgatggtg ttcagcgtgc tgagccagca tcgcctggct ctttcgaaca
     2221 agaagatcaa gctgcagctc gcgatcgccg cgatcatcgt gctggcggac gagggcaagc
     2281 tcaagctcgt gctcgagaat ctgttgtcga atgccatcaa gttcacgccg agcggcggct
     2341 cgattcacgt cggcgccgcc atgcaaggcc acgatctcgt catggacgtc agcgacgatg
     2401 gtcccggcgt g
//
LOCUS       DASSNN010000276         6263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1799779, whole genome shotgun sequence.
ACCESSION   DASSNN010000276 DASSNN010000000
VERSION     DASSNN010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799779"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1487
                     /locus_tag="VFL84_05005"
     CDS             129..1487
                     /locus_tag="VFL84_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET7608013.1"
                     /translation="MTETLEQWLDREYVHAAAAMLRSVSARELVKERRGFGQTVRPVA
                     GSIVASPVLASWDPDPDYFFHWFRDSAIVIDAVRLLHETGHLGSQAVTHFSDFVRFSL
                     SLQALDGRALFESPHWRLQVLPEFVQYLRGDDLLLVSGDAVYADARVNADGTLDISRW
                     TRPQHDGPPLRALAVLRWLRALRNPRARAIDAVSSDVLADAERLVRIDLALTAVHWRE
                     PSLDIWEEELGHHYFTLRVAAAALNEGAKWLLAANEAGEAAALLAEAQAIREALNRYW
                     LQDAGHYASRVLPAGATSTKLLDIAVILAAIHARDGEDAHSVRDPRMHATLAKLEQLF
                     DRLYPINRNRPLGRAPALGRYEGDVYFSGGAYYFSTLGAAEFCFLAAASHARGSNARA
                     KWAAHGDAFLETVRAYTPASGDLSEQFDQRSGEQTSAKHLAWSYAAFISCLTARRAVL
                     SP"
     gene            1484..3292
                     /locus_tag="VFL84_05010"
     CDS             1484..3292
                     /locus_tag="VFL84_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HET7608014.1"
                     /translation="MSDPAETRDESAFVETARAGVGAQSRMILQAILASPVGRQVIWL
                     AAALVVAILVTSYGQVILNNWNQPFYDALTRRDLNEFLYQLGVYFLIVSCLLVLDVSQ
                     RWITETFKFRLREGLTRDLLKLWMVPRRAFWLATSGSHLGVNPDQRISEDADKLCNIS
                     ADLSIGLFRSTVLFVSFAGVLWTISDDFTFRVGGVDYAVPGFMLWAAIAYAFVGSLLS
                     YYVGRNLIGRNADRYAREADLRFALVRISEHIDSIALAAGEADERRRVEMHVGNVMFA
                     MRRLVRGLTNLTWIIAGFGWISGMAPILISAPLYFSGKTSFGGMMLAVSAFTQAQGSL
                     RWFVDNFGPIADWRATLLRVANFRAALLATDSHDDAGNRIDYAEGPPHALAFEDLQVD
                     SPVGREGFREQHVVIRAGEHTLICGTPGEGKTPLFRALSGLWPWGSGRIMRPRGESFM
                     YVPRGTPYLPRGTMREALAYPTLTDRFSDRAYVQALERTGLGRYAKNLDDDRRWDREL
                     REDEQMALAFARVALHAPDWVVFDDTFSALEDDTIERIIELFTRDLTQTTIIHIGRTT
                     QAHLPLFARVLHLKKLDSEAGKEEDWATKRVRRRRR"
     gene            3289..5532
                     /locus_tag="VFL84_05015"
     CDS             3289..5532
                     /locus_tag="VFL84_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET7608015.1"
                     /translation="MKSLARAVLLVALALRPMLAAAQSFDVELPRDAADPSLAAVLHD
                     LAERVLPVYRDDDPDRYLSNVAALQMAIGDPAAARDTRRKLRERLDSEQSALPRARAT
                     VYDIYVRARAIEASEAVPFPSAYARAFEEISTGLDDLEAYRVEEWLVLPPDPFRESLQ
                     RALDERRGEGSVALDEALELVRTWLAFDAYRNFAGLVRPLLAEDTRKRYAVEQLAIPV
                     SAGATIDTELVRPRRAAGRLPTLLEFTLDRSSRDPREAAAHGYASVLTLARAAGGAAA
                     RPRPFETEGDDAWAVIAWIAQQRWSDGRVAMQGSGYGGFAAWAALKLQPPALSAIAAS
                     DPMAPGIDFPSPNRIFVSPAYRWVYGLLAPPNDTLADDDARWAELYAEWYRTGRRYRD
                     FPALPGRASAVFRSWLNHPSYDRFWQKWVPDGPELAEIDIPVLTVTGYYSAGEPAALY
                     YFAQHQANDADADHTLLIGPFDERSLARGAASSLRGLELDAAARIDPADARYEWLESV
                     LKDTERPPTLRPGINYELSGANEWRHAPSLAALEASALRFYLQAAPNGEPQRLARQRP
                     AAPLALTQTRDLADRSDSGSGPPRELVLGEVDARAGISFVTEPFDAAVDLAGRIRGEL
                     DFTINKQDVDLVLTLYELRASGEHVKLFDPPHAFRASYARDRTRRRLLLAGVRQQLPF
                     QSERMIGARFEAGSRLLLTIGINKRADQQINYGAGDDVSEESIEDAGPPVRIRWHEGS
                     FIEIPAQ"
     gene            complement(5596..5808)
                     /locus_tag="VFL84_05020"
     CDS             complement(5596..5808)
                     /locus_tag="VFL84_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608016.1"
                     /translation="MNNWDALRLRGSDRRFRPEEMPVDYYGVGVELVPRTVEGLVTWD
                     VSGHAQCEKDRLSSAYRSTIKTRGGY"
BASE COUNT          962 a         2132 c         2121 g         1048 t
ORIGIN      
        1 ccgatcatcg tcatgatcga gaaccaccgg tcggggctct tgtggaacct cttcatggcc
       61 gtgcccgagg tgcgggccgg cttgaggcga ctcggtttct cgagcccgca tctcgaagaa
      121 gcggagcggt gaccgagacg ctcgagcaat ggctcgaccg cgagtacgtc catgccgcgg
      181 ccgccatgct gaggagcgtt tcggcgcgcg agctcgtcaa ggagcgccgc gggttcgggc
      241 agaccgtcag acccgtcgcc ggctcgatcg tcgcttcgcc cgtgctcgcg tcgtgggatc
      301 ccgacccgga ttatttcttt cactggttcc gcgactcggc gatcgtgatc gacgccgttc
      361 gcctgcttca cgaaacgggt catctgggct cgcaggcggt cacgcatttc tccgacttcg
      421 tgcgcttcag cctgtcactg caggcgctcg acgggcgcgc gctcttcgag tcgccgcact
      481 ggcgtttgca ggtgctgccg gagttcgtgc agtacctgcg cggcgacgac ttgttgctcg
      541 tctcgggcga cgccgtctac gccgacgcgc gcgtgaacgc ggacggcacg ctcgatattt
      601 cgcgctggac gcggccgcag cacgacggtc cgccgcttcg cgcattggcc gttctgcgct
      661 ggctgcgagc gctgcgcaat ccgcgtgccc gcgcgatcga cgccgtgtcc tccgacgtgc
      721 tggccgatgc cgagcggctc gtgcggatcg atctcgcgct gaccgccgtg cactggcggg
      781 agccgtcgct cgacatctgg gaagaagagc tcggccatca ctatttcacg ctccgcgtcg
      841 cggcggcggc gttgaacgaa ggcgcgaaat ggctcttggc agcgaacgaa gcgggcgaag
      901 ccgccgctct gctcgccgaa gcgcaggcga tccgcgaggc gctcaaccgg tattggcttc
      961 aggacgcggg ccactacgcc tcgcgcgtat tgcccgccgg cgccacgtcg acgaagctgc
     1021 tcgacatcgc cgtgatactg gcggcaattc acgcgcgcga cggcgaggat gcgcactcgg
     1081 tccgcgatcc gcgcatgcac gccacgctcg ccaagctcga gcagctcttc gatcggctct
     1141 atccgatcaa tcgcaaccgg ccgctcgggc gcgcgcccgc gctcggccgc tacgaaggcg
     1201 acgtctactt ttccggcggc gcctactatt tctcgacgct cggcgccgcg gaattctgtt
     1261 tcttggctgc cgcgtcgcac gcgcgtggca gcaacgcgcg cgcgaagtgg gccgcgcacg
     1321 gcgacgcgtt cctcgaaacc gtccgcgcct ataccccggc gagcggcgat ttgtccgagc
     1381 aattcgacca gcgcagcggc gagcagacgt cggcgaaaca cctcgcgtgg agctatgccg
     1441 cattcatctc atgcctcacg gcgcgccggg cggtgctctc gccatgagcg accccgcgga
     1501 gacgcgcgac gagtcggctt tcgtcgagac cgcgcgcgcc ggggttggcg ctcaatcccg
     1561 catgattttg caggcgatcc tcgcgtcgcc ggtcggacga caagtgatct ggctcgccgc
     1621 ggcgttggtt gttgcaatcc tcgtcacgag ctacgggcag gtcatcttga acaactggaa
     1681 tcagccgttc tacgacgcgc tcacgcgccg cgacttgaac gagttcctct accagctcgg
     1741 cgtgtacttc ctgatcgtct cgtgccttct cgttctcgac gtatcgcagc gctggatcac
     1801 cgagactttc aagttccggc ttcgcgaagg cttgacgcgc gatttgttga agctctggat
     1861 ggtcccgcgt cgtgctttct ggctcgcgac gagcggcagt cacctaggag tgaatccgga
     1921 tcagcggatc agcgaggatg cggacaagct gtgcaacatt tccgccgatc tgtcgatcgg
     1981 actcttccgt tcgaccgtgc tgttcgtgag cttcgcggga gtgctctgga cgatttccga
     2041 cgacttcacg tttcgcgtcg gcggcgtcga ttacgccgtg ccggggttca tgctgtgggc
     2101 tgcgatcgcc tatgcattcg tcggatcgct gctctcttac tacgtgggcc gaaacctgat
     2161 cggccgcaac gccgaccgct atgcgcgcga ggcggacctg cggttcgcgc tcgtccgcat
     2221 cagcgagcac atcgacagca tcgcgcttgc ggccggcgaa gccgacgagc ggcgccgcgt
     2281 cgagatgcac gtcggcaacg tcatgttcgc gatgcgccgg ctcgttcgcg gcctcacgaa
     2341 cctgacgtgg atcatcgcgg gattcggctg gatctcgggc atggcgccga ttttgatctc
     2401 ggcaccgctc tacttcagcg ggaagacgtc gttcggcggc atgatgctgg ccgtctccgc
     2461 gttcactcag gcgcaggggt cgctgcggtg gttcgtcgat aacttcggcc cgatcgcgga
     2521 ctggcgagcc acgctgctgc gcgtcgcgaa cttcagagcc gccctgctcg ccaccgactc
     2581 acacgacgac gccggaaacc gcatcgacta cgcggagggt ccaccgcatg cgttggcatt
     2641 cgaagatcta caagtcgatt ccccggttgg gcgcgagggg tttagggagc aacacgtcgt
     2701 gatacgcgcc ggcgagcaca cgctgatctg cggaacgcca ggcgagggga agacgcctct
     2761 gttccgcgcg ctgtcgggcc tatggccctg gggcagcggc cgcatcatgc ggccgcgcgg
     2821 cgaatcgttc atgtacgtgc cgcgcggcac gccgtacctg ccgcgcggca cgatgcgcga
     2881 ggcgctcgct taccccacgt tgacggaccg cttctcggac cgcgcttacg tgcaagcgct
     2941 cgagcgaacg gggcttggac ggtacgcgaa gaacctcgat gacgaccggc gctgggaccg
     3001 agagctccgc gaggacgagc agatggcgct ggcgttcgcg cgcgtggcgt tgcacgcgcc
     3061 cgactgggtc gtgttcgacg acacgttctc cgcgctggaa gacgacacga tcgagcgcat
     3121 catcgagctg ttcacgcgcg atctcacgca gacgacgatc attcacatcg ggcgcaccac
     3181 gcaggcgcat ctgccgctgt tcgcgcgcgt gctgcatctg aagaagctcg acagcgaggc
     3241 cggcaaggaa gaagactggg cgacgaaacg agtgcggcgt cgacggcgat gaaaagcctc
     3301 gctcgcgccg tgttgcttgt tgcgttggcg ttacggccga tgctcgcggc cgcgcagagc
     3361 ttcgacgtcg agctgccgcg cgacgccgcc gatccatcgc tcgcggccgt cttgcacgat
     3421 ctcgccgagc gcgtcttgcc ggtctaccgg gacgacgatc ccgatcgcta tctctcgaac
     3481 gtagcggcgc tgcagatggc gattggcgac ccggccgcgg cgcgcgacac gaggcggaag
     3541 ctcagggagc ggctcgacag cgagcaaagc gccctgcccc gggcgcgcgc caccgtctat
     3601 gacatttacg tgcgagcgcg cgcgatcgaa gcctcggagg ccgtcccgtt tccgagcgcg
     3661 tatgcgcggg cattcgagga gatttcgacg gggctcgacg atctcgaggc ctatcgcgtc
     3721 gaggagtggc tggttctgcc ccccgaccca ttccgcgaaa gcctccagcg cgcgctcgac
     3781 gagcgccgcg gcgaaggctc ggtcgcgctc gacgaagcgc tcgagctcgt gcggacgtgg
     3841 ctcgcgttcg acgcgtaccg aaacttcgcg ggcctcgtgc ggccgctgct cgccgaggac
     3901 acgcggaaac gctacgccgt cgagcagctc gcgatcccgg tctccgccgg cgcgacgatc
     3961 gacaccgagc tcgtccggcc gcggcgagcg gccgggagac tgccgacgct gctcgagttc
     4021 acgctcgacc gctcgagccg cgatccgcga gaggctgccg cgcacggcta cgccagcgtg
     4081 ctcacgctcg cgcgcgccgc gggcggcgca gccgcgcgcc cgcgcccgtt cgagaccgaa
     4141 ggagacgacg cctgggccgt catcgcttgg atcgcacagc agcggtggag cgacggccgc
     4201 gtcgcaatgc agggctccgg gtacggcggc ttcgccgcat gggccgccct gaagctgcag
     4261 ccgccggcgc tgagcgcgat cgcggcgtcc gatccgatgg cgcccggtat cgatttcccg
     4321 agccccaatc gaatcttcgt gagcccggcc taccgctggg tgtacggcct gctggcgccg
     4381 ccgaacgaca cgctcgcgga cgacgacgcc cgctgggccg agctctatgc cgagtggtat
     4441 cgaacggggc gccggtatcg cgactttccg gcgctgcccg gccgcgcctc ggcggtcttt
     4501 cgaagctggc tgaaccatcc gagctacgac cggttctggc agaagtgggt gccggacggg
     4561 cccgagctcg cggaaatcga cattcccgtg ctcacggtca ccggttacta ctcggcgggc
     4621 gagccggcgg cgctgtatta cttcgcgcag caccaggcga acgacgccga tgccgaccac
     4681 acgctgctga tcggcccgtt cgacgagcgc agcctcgcgc gcggcgccgc gtcgtccctt
     4741 cgcggcctcg agctcgacgc ggccgcccgc atcgatccgg ccgacgcgcg ttacgaatgg
     4801 ctcgagagcg tgctcaagga cacggagcgc ccgccgacgc tgcgcccggg catcaactac
     4861 gagctgtcgg gcgcgaacga atggcgtcac gcgccgtcgc tcgccgcgct cgaagcgagc
     4921 gcgctgcgct tttaccttca ggctgcaccg aacggagagc ctcaaaggct cgcgaggcag
     4981 cgccccgcgg cgccgctggc gctgacgcag actcgcgatc tcgcggatcg cagcgactcc
     5041 ggctcggggc cgccgcgcga gctcgttctc ggcgaggtcg acgcgcgggc ggggatctcg
     5101 ttcgtgacgg agccgttcga cgcggccgtc gatctcgcgg gccgcattcg cggcgagctc
     5161 gacttcacga tcaacaagca ggacgtcgac ctggtcctga cgctgtacga gctgcgcgcg
     5221 agcggcgagc acgtcaagct cttcgacccg ccgcatgcgt ttcgagcgag ctacgcgcgc
     5281 gaccgcacgc gtcgccgcct gctcctggcc ggcgtcagac agcagttgcc gtttcagagc
     5341 gagcgaatga tcggggctag gttcgaggcc ggcagtcgct tgctgctgac gatcggcatc
     5401 aacaaacgcg cggatcagca gatcaattac ggcgccggcg acgacgtcag cgaagaatcg
     5461 atcgaggatg ccgggcctcc ggtccgaatc cgttggcacg aaggcagctt catcgagatc
     5521 ccggcgcaat gagccggccg cgagctgctt cgcggggtcg cgccgtccgc ggcgatcgca
     5581 aacgcttgat cgcggctaat atccgccgcg cgtcttgatc gtgctccgat aggcggagct
     5641 gagccggtcc ttctcgcact gcgcgtgccc cgagacgtcc cacgtcacga ggccctcgac
     5701 ggtcctcgga acgagctcga cgccgacacc gtagtaatcg acgggcattt cttccggccg
     5761 gaaccggcga tcgctgccgc gcagtctcaa cgcgtcccaa ttgttcatct caaagccttt
     5821 gttgtgatcg aggcggtgta gcgtcgctgc gaaaaatgtg ccaccgacgc ccggggcgcc
     5881 gacggggtgc tttggcgcgt cgcgccgcgg cgggaacgcc cacgtgcctc caggtgcggc
     5941 gcgccggacg acagggcgaa ggcccgagat ggaagcgaat tgcaaaaatt ggtatggccg
     6001 gcgggcccgg ggaccttcgg agcgccctag cccgaaacga gcggctggcg ctatggtgcg
     6061 gcattggggg cgccggtgtg aacgccggcc gtacggcctg gaaccaagct gtaggcggag
     6121 ccaaagctcg tggcgccgac ccgcgccgcc gatccgtgcc gtctggagga cgcttcatgt
     6181 tcgtacgcgg attgggcacg gcgcatcccg cgcggcgcta cacgaaagcc gactgtctcg
     6241 aggcgttccg gagctccgcc tgg
//
LOCUS       DASSNN010000277         3736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_663849, whole genome shotgun sequence.
ACCESSION   DASSNN010000277 DASSNN010000000
VERSION     DASSNN010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663849"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /locus_tag="VFL84_05025"
     CDS             <1..1788
                     /locus_tag="VFL84_05025"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET7608017.1"
                     /translation="TLDRLDVDEKVLGPWRLESPAALQLGRGLVTVATACLVHVSNAR
                     WCSELDVQGRPEDRLVVSGQSFDLATLRPLLPPEVELAGVYQLSGSLFDLMGDPRGAV
                     ALTGGTTRARVVAGEGVFETELDLVQAGMTLTSGRLALTAAVRRTAGGSADVNAEIAD
                     VRARDSRVDGAVRVEWPDVGFLTLLSPELERVGGSIAVNLDVAGTVNEPTLDGRAVFS
                     NGRIDVPRWGLAIEGIEATATSSEGRALAIEGTGRAGDGVLSVAGTTELDPESGWPTE
                     LTLRGDTVRVVQLPNVEIFASPDLRVNVALPLIEVTGRVHIPRATATLDVLPSQAVTP
                     SPDAVVHGAQAAARSRPLQLKTAIELTLGDDVHYSGLNLDTTVTGELRVATDPNASTT
                     ATGTLRLDGTYDAYGQKLTLERGQLLFSGPLDNPGLDVRALRMLENQDFSSGVTEVGV
                     ELTGTLKAPRTRVFSTPSMTEADALSYLLFGRPMSGSNAGLGTEETSALQTAALSLGL
                     QQALPVVQRIGNTLGLDELTVQSTDTDAGALMAGKYLSPRVYIRYSYGLFNRIGGFLL
                     RFKVNDRLSIETRSGEQESMDLFYTIEKE"
     gene            1879..3093
                     /locus_tag="VFL84_05030"
     CDS             1879..3093
                     /locus_tag="VFL84_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608018.1"
                     /translation="MRRIPLVTAALVAAATLPCHGQAPKRFMGGPVTLEDQGSFFVGG
                     VTKVSEYAAVPGAPPGQAPPPRTPQQITIGQMYVQFQIPARRSGWPVIMVHGSTHTGA
                     ALESTPDGREGWYPYFVRKGVATYVVDQSGRGRSGFDQSVLHEADALLAAGNRDAAAA
                     LLPTFQRITDNGAYQAWFGHLLPAGSTIETGKLVPHGSPEDPQGPGTAAEPHVAPLFP
                     LAAVDEYYKQLVPNAEATLPGSICKTCMPQELSPANTWTPENLATLVERLGGAIVATH
                     SQSGIMGHHMVRILKERGHLGVVKGLITIEGGCSFAQSGLTAEDFDAIPYLALKGDYT
                     ATSTQCQESVDAINARRAQGQGTARAEYMKLDERGMLGVTHMMMLDSNNLEIADLMLE
                     WVGEHVPRAPRR"
     gene            3112..>3736
                     /locus_tag="VFL84_05035"
     CDS             3112..>3736
                     /locus_tag="VFL84_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608019.1"
                     /translation="MQHPPITLRYFDARSRAQFLRYYFKARKVSFTDDRVPVNDEFAA
                     WRALRDDRARTGPFHKLPVLHWGDRLLAETLMIAAFVHEASGDAGSLNDDDNLRHGML
                     TSSLCLDMMNPIAILLWLEINYQGADVAAVAKRTLDRLKGHCQSLDQAFNDWRWLDKL
                     RNRRIMLADCLLWEQLDVARVVFGPHWSLSSTPTLARFYDDFPGRQIC"
BASE COUNT          585 a         1328 c         1265 g          558 t
ORIGIN      
        1 acgctcgatc ggctcgacgt cgacgagaaa gtgctcggac cgtggcgcct cgagtcgccg
       61 gcggccttac agctgggccg cgggctcgtg acggttgcga cggcctgtct cgtccacgtc
      121 tcgaatgcgc gctggtgcag cgagctcgat gtgcaaggcc ggcccgagga tcggctcgtc
      181 gtgtccgggc agagcttcga cctcgcgacg ttgcggccgc tgctgccgcc cgaggtcgag
      241 ctcgcgggcg tctaccagct ttcggggtcg ctgttcgacc tcatgggcga cccgcgcggc
      301 gccgtcgcgc tgacgggcgg caccacgagg gcgcgcgtcg ttgccggcga gggcgtgttc
      361 gagacggagc tcgatctcgt gcaggccggc atgacgctga cgagcggccg cctcgcgctc
      421 accgcggccg tgcgcaggac cgcgggcggc agcgccgacg taaacgccga gatcgccgac
      481 gtgcgcgcgc gcgactcgcg cgtcgacggc gccgtgcgcg tcgagtggcc cgacgtcggg
      541 ttcctcacgc tgctctctcc cgagctcgag cgcgtcggcg gctcgatcgc cgtgaacctc
      601 gacgtcgccg gcacggtgaa cgagccgacg ctcgacggcc gcgcggtttt ctcgaacggc
      661 cgcatcgacg tgccgcgctg ggggctcgcg atcgaaggca tcgaggcgac cgcgacgagc
      721 agcgaaggcc gcgcgctcgc aatcgagggg acgggccgcg ccggcgacgg cgtgctgagc
      781 gtcgcgggca cgaccgagct cgacccggag tcgggatggc cgaccgagct cacgctgcgc
      841 ggcgacacgg tccgcgtcgt gcagctgccg aacgtcgaga tcttcgcgtc gccggacctg
      901 cgcgtgaacg tcgctttgcc actgatcgag gttaccggcc gcgtgcacat cccgcgcgcc
      961 actgccacgc tcgacgtgtt gccgtcgcag gccgtgacgc cgtcgccgga cgccgtcgtg
     1021 cacggcgcgc aagcggcggc gcgctcgcgg cccctgcagc tcaagaccgc gatcgagctc
     1081 acgctcggcg acgacgtgca ctactcgggc ctcaacctcg acacgaccgt caccggcgag
     1141 ctgcgcgtcg cgaccgatcc gaacgcgtcg acgaccgcca cgggcacgtt gcgcctcgac
     1201 ggcacctacg acgcctacgg ccagaagctc acgctcgagc gcggccagct cctcttcagc
     1261 ggcccgctcg acaatccggg gctcgacgtc cgcgcgttgc gcatgctcga gaaccaggac
     1321 ttcagcagcg gcgtgaccga ggtcggcgtc gagctgacgg gcacgctgaa ggcgccgcgt
     1381 acgcgcgtgt tctcgacgcc gtccatgacc gaggccgacg cgctctcgta cctgttgttc
     1441 ggccggccga tgagcggctc gaacgcgggc ttgggcaccg aggaaacctc ggcgctccag
     1501 acggccgcgc tatcgctggg attgcagcag gcgctgcccg tcgtgcagcg catcggcaat
     1561 acgctcggcc tggacgagct gaccgtgcag tcgacggata ccgacgccgg cgccctgatg
     1621 gcgggcaaat acttgagccc gcgcgtctac atccgctaca gctacgggct gttcaaccgc
     1681 atcggcggct ttctgctgcg cttcaaggtc aacgaccgct tgagcatcga gacccgctcc
     1741 ggcgagcagg aatcgatgga cctgttctac acgatcgaga aggaatagcg gtacagcgcg
     1801 ccggggcgtt tcgggaacag ggcccgtcgt ggcatgctgc gccgcataga gaccctgcca
     1861 agaagaggtt ttcgacgcat gcgacgcatc cctctcgtga cggccgccct cgtcgcggcc
     1921 gcgacgctgc cgtgccacgg ccaagcgccg aagcgcttca tgggcggccc cgtcaccctc
     1981 gaggaccaag gcagcttctt cgtcggcggc gtcacgaaag tcagcgaata cgccgccgtg
     2041 cccggcgcgc cgcccggcca agcgccgccg ccgcgcacgc cgcagcagat cacgatcggc
     2101 cagatgtacg tgcagttcca aatcccggcg cggcgctcgg gctggcccgt catcatggtc
     2161 cacggctcga cgcatacggg cgccgcgctc gagtcgacgc ccgacggccg cgaaggttgg
     2221 tacccctatt tcgtgcgtaa aggtgtcgcg acctacgtcg tggatcagtc ggggcgcggc
     2281 cgatccggct tcgatcagtc cgtgctgcac gaggccgacg cgctgctggc ggccggcaac
     2341 cgcgatgccg ccgccgcgct gctgccgacg ttccagcgca tcaccgacaa cggcgcgtat
     2401 caggcctggt tcgggcacct gctcccggcc ggctcgacga tcgagaccgg caagctcgtc
     2461 ccgcacggct cgcccgagga tccgcaaggc ccgggcaccg cggccgagcc gcacgtcgcg
     2521 ccgctgttcc cgctcgcggc cgtcgacgag tactacaagc agctcgtgcc gaacgcggag
     2581 gcgacgctgc cgggctcgat ttgcaagacc tgcatgccgc aggagctgtc gccggcgaac
     2641 acgtggacac cggagaactt ggcgacgctg gtcgagcggc tcggcggcgc gatcgtcgcg
     2701 acgcattcgc agtccgggat catgggccac cacatggtcc gcatcttgaa ggagcgcggc
     2761 cacttaggcg tagtgaaggg cctgatcacg atcgaaggcg gttgctcgtt cgcgcagagc
     2821 gggctcaccg ccgaagattt cgacgcgatc ccgtatctcg cgctgaaagg cgactacact
     2881 gcgacaagca cgcagtgcca agagtccgtc gacgcgatca acgctcgccg cgcgcagggc
     2941 caaggcactg cgcgcgccga gtacatgaag ctcgacgagc gcggcatgct cggcgtcacg
     3001 cacatgatga tgctcgattc gaacaacctc gagatcgccg atctgatgct cgagtgggtc
     3061 ggcgagcacg tgccgcgcgc gccgcggcgc tgacagtcaa cccgaggacc tatgcaacat
     3121 ccgccgatca cactgcgcta tttcgacgcg cgctcgcgcg cgcaattcct gcgctattac
     3181 ttcaaggccc gcaaggtttc gttcacggac gaccgagtgc ccgtcaacga cgagttcgcc
     3241 gcgtggcgcg cgctgcgcga cgaccgcgcg cgcacggggc cgttccacaa gctgcccgtt
     3301 ttgcattggg gcgaccggct gctcgccgag acgctgatga tcgccgcgtt cgtgcacgag
     3361 gcgagcggcg acgccggctc gttgaacgac gacgacaacc tgcgccacgg catgctgacg
     3421 tcgagcctct gcctcgacat gatgaacccg atcgcgatcc tgctgtggct cgagatcaac
     3481 tatcagggcg ccgacgtcgc cgccgtcgcg aagcgcacgc tcgaccgctt gaaaggccac
     3541 tgccaatcgc tcgatcaggc gttcaacgac tggcgctggc tcgacaagct gcgcaaccgc
     3601 cgcatcatgc tcgccgactg cctgctttgg gagcagctcg acgtcgcgcg cgtcgtgttc
     3661 ggaccgcatt ggtcgctctc ctcgacgccg acgctcgcgc ggttctacga cgacttcccg
     3721 ggccgccaga tctgcg
//
LOCUS       DASSNN010000278         2594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1436195, whole genome shotgun sequence.
ACCESSION   DASSNN010000278 DASSNN010000000
VERSION     DASSNN010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436195"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..561
                     /locus_tag="VFL84_05040"
     CDS             175..561
                     /locus_tag="VFL84_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7608020.1"
                     /translation="MLTNSKLQSIIWTSRIAEAETFYREVLGLTLRTKSDGALVFDVG
                     GSDLRVAPIPATTRGEHTVIGFAVPDVDAAIEWLGSRGVAFERFAGFPHAANGTVLSP
                     DGAKVAWFRDPDGNILSVVQFATADR"
     gene            complement(608..2437)
                     /gene="typA"
                     /locus_tag="VFL84_05045"
     CDS             complement(608..2437)
                     /gene="typA"
                     /locus_tag="VFL84_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET7608021.1"
                     /translation="MLPTDKLRNIAIIAHVDHGKTTLVDKLLQQSGTIDQRIALPERV
                     LDSNELERERGITILSKNTAIRWEGWRINIVDTPGHADFGGEVERVLSMVDGVLLLVD
                     AVDGPMPQTRFVTQKAFLRGFTPLVVINKIDRDGARPDWVLDQTFELFDRLGASDKQL
                     DFPVIYASALKGYASKEPTARDGDMQPLFEAIVSNVPPPPVELEGPLQLQVSSLDYNS
                     YVGLMGIGRISRGVVKRNMPVTLVQRDGTERNAQLMELYGFEAMTRVAVPEAGAGDIV
                     AFSGIEGLQISDTVCARGHGEGLPTLQVDEPTITMTFQVNKSPFAGQEGKYLTSRQIR
                     ARLDQELQHNVALRVEDTDDADKFRVSGRGELHLSVLIENMRREGYELAVSRPAVIEH
                     VVDGAVHEPWEQVTVDFAEQYQGAVMTRLAERKGELISMMPDGRGRVRLEYRIPARGL
                     IGFRTEYLTATAGTGLLYHIFDGYSPRAKGEIGQRINGALVSMVQGKALGYALFNLQE
                     RGALFIGHGVEVYEGMIIGIHSRDNDLMVNPTKGKKLTNIRAAGSDENIILTPPIQFS
                     LEQALEFIDDDELVEVTPTSIRLRKKLLTENERKREQRKAADG"
BASE COUNT          390 a          844 c          873 g          487 t
ORIGIN      
        1 ccgggtacga tcgcggcgca gcggcgccga gcttgacgac cgtcgcgtcg gaccgctaac
       61 gtaactcccg caattagccg cgctcgtcgc ggcttttctg tttctgtgcc gcggccgcgg
      121 catcgcttct tttgtcgggc caactcatcg gcccatcaac gaaaggagcg aacagtgctg
      181 acgaattcca aattgcaatc gatcatctgg acgtcgagga tcgcagaggc cgagacgttc
      241 taccgcgaag tgctcgggct tacgcttcgg acgaagtccg acggcgccct cgtcttcgac
      301 gtcgggggca gcgatttgcg cgttgcgccg atccccgcga cgactcgcgg cgagcacacg
      361 gtcatcggct ttgcggtccc cgacgtcgat gccgcgatcg agtggctcgg ttctcgcggc
      421 gtcgccttcg agcgattcgc cgggttcccg cacgccgcca atggcaccgt gctttcgccg
      481 gacggagcca aggtcgcttg gtttcgcgat cctgacggga acattctttc ggtcgtgcag
      541 tttgcgacgg cggatcgctg attccgcgcg cagagggggc caaggacggc cctccgcggc
      601 agcgtgctca gccgtccgcg gccttgcgct gctcgcgctt gcgctcgttc tccgtgagca
      661 gcttcttgcg caagcggatg ctcgtcggcg tgacctcgac gagctcgtcg tcgtcgatga
      721 actcgagcgc ctgctcgagc gagaactgga tcggcggcgt caggatgatg ttctcgtcgg
      781 agccggccgc gcggatgttc gtgagcttct tgcccttcgt cggattcacc atgaggtcgt
      841 tgtcgcggct gtggatgccg atgatcatcc cttcgtagac ctcgacgccg tggccgatga
      901 aaagcgcgcc gcgctcctgc aagttgaaca gcgcgtagcc gagcgccttg ccttgcacca
      961 tcgagacgag cgcgccgttg atgcgctggc cgatctcgcc cttcgcgcgc ggcgaatagc
     1021 cgtcgaagat gtggtacagc aatcccgtgc ccgcggtcgc cgtcagatac tcggtgcgaa
     1081 agccgatcag tccgcgcgcc gggatccggt actcgagccg cacgcggccg cgcccgtccg
     1141 gcatcatcga gatgagctcg cccttgcgct ccgcgaggcg cgtcatgacg gcgccctgat
     1201 actgttccgc gaaatcgacc gtgacctgct cccacggctc gtgcaccgcg ccgtcgacga
     1261 cgtgctcgat gacggccggc cgcgataccg cgagctcgta accttcgcgc cgcatgttct
     1321 cgatcagcac cgacaggtgc agctcgccgc ggcccgacac acggaatttg tcggcgtcgt
     1381 ccgtgtcctc gacgcgcagc gcgacattgt gctgaagctc ttggtcgagc cgcgcgcgaa
     1441 tctggcggct cgtgaggtac ttgccctcct ggcccgcgaa cggcgacttg ttgacttgga
     1501 acgtcatcgt gatcgtcggc tcgtcgactt gcagcgtcgg caggccttcg ccgtggccgc
     1561 gcgcgcaaac cgtgtccgag atctgtaggc cctcgatgcc gctgaacgcg acgatgtcgc
     1621 cggcgccggc ctcgggtacc gcgacgcgcg tcatggcttc gaaaccgtac agctccatga
     1681 gctgcgcgtt gcgctcggtg ccgtcgcgct gcacgagcgt gaccggcatg ttgcgcttca
     1741 cgacgccgcg gctgatgcgg ccgatgccca tcaggccgac gtacgagttg tagtcgaggc
     1801 tcgagacctg cagctgcagc ggtccttcga gctcgacggg cggcggcggg acgttgctca
     1861 cgatcgcctc gaacagcggc tgcatgtcgc cgtcacgtgc ggtcggttcc ttcgacgcat
     1921 agcccttcag cgcggacgcg tagatgactg gaaagtcgag ctgcttgtcg ctcgcgccga
     1981 gccggtcgaa cagctcgaac gtttgatcga gcacccaatc cggacgcgcg ccgtcgcggt
     2041 cgatcttgtt gatgacgacg agcggcgtga agccgcgcaa gaacgccttc tgcgtgacga
     2101 agcgcgtctg cggcatcgga ccgtcgacgg cgtcgacgag gagcagcacg ccgtcgacca
     2161 tcgacagcac gcgctcgacc tcgccgccga agtcggcgtg tccgggcgtg tcgacgatgt
     2221 tgatgcgcca gccttcccag cggatggcgg tgttcttcga caggatcgtg atgccgcgct
     2281 cgcgctcgag ctcgttcgag tcgagcacgc gctcgggaag cgcgatgcgc tgatcgatcg
     2341 tgcccgactg ctgcagcagc ttgtcgacga gcgtggtttt gccgtgatcg acgtgggcga
     2401 tgatcgcaat gttgcggagc ttgtcggtgg ggagcatagg gggcgcgggg ttgagggtgg
     2461 cgcattatag ggccccgcgc gccgggggaa atggggtcgg agcggatttt gacggatttt
     2521 tcgcggcgag catgtcttgc aggtccgcgg ccatcccgaa gggacgccgg acgcgccgcc
     2581 cgactgaaaa cccg
//
LOCUS       DASSNN010000279         5948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_793937, whole genome shotgun sequence.
ACCESSION   DASSNN010000279 DASSNN010000000
VERSION     DASSNN010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793937"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFL84_05050"
     CDS             complement(<1..561)
                     /locus_tag="VFL84_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET7608022.1"
                     /translation="MFVKICGMNSPEAVEAAVAAGVDALGFVFAESPREVTPEQAKSL
                     CASVPAHITRIAVMRHPHKEHWERVVEVFEPNWLQTDAEDFRWIKLPPGCTALPVYRD
                     SNAPADSDLPDRLLFEGTHSGSGRVADWDEARGLARVAEVILAGGLTAANVAEAVSFV
                     RPWGVDVSSGVERARGEKDPDKIREFV"
     gene            complement(552..1385)
                     /locus_tag="VFL84_05055"
     CDS             complement(552..1385)
                     /locus_tag="VFL84_05055"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET7608023.1"
                     /translation="MTFLERMAKASRERVRAARAKESEAKLERRALDAPAAPPLGLDR
                     FDVIAELKLRSPSAGGLARGDFDSRAQLAGYARGGAAAVSVLTEPTEFRGELEHLAAA
                     AEALRPKRPVMRKDFLVDPYQVLEARAAGAGGVLVILAMLDDAAVRALVDCARGLSMF
                     VLLEAFDRADLERLSAYDAPRAGGSPLLAGVNCRDLRDLEVRFERFAELGPYLPRHLP
                     TVAESGIVNAGDVEAVVAAGFRLALVGSSLMRASDPAAALAGLVAAGRAAVAGRQARC
                     S"
     gene            complement(1382..2410)
                     /gene="trpD"
                     /locus_tag="VFL84_05060"
     CDS             complement(1382..2410)
                     /gene="trpD"
                     /locus_tag="VFL84_05060"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET7608024.1"
                     /translation="MSAEAPKLLERLLRGEHLDEATAGELLRLLARGEIQPALAGALL
                     AALRAKGETPEEIRGFARAMRELALRPAIPAGAYVDIVGTGGDGSGSLNLSTGASLLA
                     AACGLPVVKHGNRSVSSKSGSADVLAALGLPIPLDEARAGACFERCGFTFLFAPHYHP
                     ATKSIAAVRQALGVRTVFNILGPLANPAAPPFHVIGAFSDAMAASMADTLAGLPLTRA
                     FVVHGEAGWDEATPAGPFVCYDVRPGRVERTLREPRDAGIERCTLDELRGTDAAGNAA
                     RLREALAGRDTAAHRDALVLNAALALEVTGEEPDARSAVARARKSLASGAGTRLLDRI
                     AAFAKEKP"
     gene            complement(2407..2979)
                     /locus_tag="VFL84_05065"
     CDS             complement(2407..2979)
                     /locus_tag="VFL84_05065"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET7608025.1"
                     /translation="MSVRLLLIDNYDSFTYNLVQAFLVLDAEVLVYRNDALSVADAAK
                     LKPTHLVISPGPGRPDDAGSSLAMIGAFAGKIPVLGVCLGHQSIVQHFGGEIIGAAAL
                     MHGKTSVVQHDGATIYRGLSNPFEVGRYHSLAANREKMPPDLVVTARTTDGEIMGVRH
                     RKLAVEGVQFHPESVLTPEGPHLLKNFLHP"
     gene            complement(2976..4361)
                     /locus_tag="VFL84_05070"
     CDS             complement(2976..4361)
                     /locus_tag="VFL84_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HET7608026.1"
                     /translation="MESPFDIAADLDTPVSAYMKLKPFKPRFLLESVESGERLARYSF
                     IGLGEAVTVELHSDRLLIDGKAARRPTNAAEWQTMMRTALERAPLLKPQIEGIPFDGG
                     LVGTTGYDVVRFFERLPPNRAERDGLPQAAYVAPESLLVFDHLTRRVALLSAGDERER
                     QALRTAVVKALRGGLDAPDRPGKFSPAAASMTQQEHAASVLKGKAHIKAGDIYQIVLS
                     VRFAGRTALDAFQTYRGLRLLNPSPYMYFLELGKLRVAGSSPEALVRLTGRTASLRPI
                     AGTRPRGLTPEHDLALERELLADPKENAEHVMLVDLARNDLGRVASSGSVHVEPYRKI
                     ERYSHVMHIVSGVQGLVAPDRDAFDLFAAAFPAGTLVGAPKVRAMQLIAELEPTGRGL
                     YGGTVGYFAKNGNMDQAITIRTMVFNGDEYSFQAGGGIVADSSPEMEYQEVLAKSAIL
                     RRALELAEEGL"
     gene            4614..>5948
                     /locus_tag="VFL84_05075"
     CDS             4614..>5948
                     /locus_tag="VFL84_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7608027.1"
                     /translation="MSSLPGRHAPLARAGAEKRSGRQALDDYLSFWRYSRRAITLVWS
                     THRGLTIALGLLTIAAGLMPAGIAYVGKLIVDAVVVAIDLNRAGAAPDYARVLELVAL
                     EGVLVAALSGAQRGIGFCQSLLRVLLSQRVNLLILDKALTLTLAQFEDSELYDKLNRA
                     RQEASVRPLSLVNRTFALAQNLVSLASYAGLLLAFSPWAVAILVAAGLPAFIAETRFS
                     GERFRAFQWRSQDRRMLGYLEIVIAREDHAKEVKLFDLGARLLARFRDIFRRIYEEEK
                     RLTLRRDGWGFVLGLVASAAFYGAYVWIALATIDGTISLGDMTMYLLLFRQGQSAVAA
                     MLSAIGGMYEDNLYLSNLYDYLEQPSAGIHGTATTGLNPQAGIEFDDVTFQYPGATNA
                     ALRDVTLQVRPGQSVALVGSNGSGKTTLVKLLAGLYTPTRGVVRYQGRDLRDW"
BASE COUNT          886 a         2076 c         2074 g          912 t
ORIGIN      
        1 gacgaactca cgaatcttgt ccggatcttt ctcgccgcgc gcgcgctcga cgccgctcga
       61 gacatcgacg ccccaggggc gcacgaagct caccgcctcg gcgacgttcg cggccgtcaa
      121 cccgcccgcg aggatgactt cggcgacgcg cgcgaggcca cgcgcctcgt cccagtccgc
      181 gacgcggccg ctgcccgagt gcgtgccttc gaacagcagc cggtccggca agtccgagtc
      241 ggccggcgcg ttgctgtcgc gatagaccgg caacgccgtg cagcccggcg gcagcttgat
      301 ccagcggaaa tcctcggcgt cggtctgcag ccaattcggc tcgaagacct cgacgacgcg
      361 ctcccagtgc tccttgtgcg gatgccgcat gacggcgatg cgcgtgatgt gcgcggggac
      421 cgaggcgcag agcgacttcg cttgctcggg cgtgacctcg cgcggcgact cggcgaacac
      481 gaacccgagc gcgtcgacgc cggctgcgac ggcggcctcg acggcttcgg ggctgttcat
      541 cccgcagatt ttcacgaaca tcgtgcttgc ctccctgcga cggcggcccg gcccgcggcg
      601 acgagccccg cgagcgcggc cgccggatcc gaggcgcgca tcagcgatga cccgacgagc
      661 gccaagcgga agcccgccgc gacgacggct tcgacgtcgc cggcattgac gatgccgctc
      721 tcggcgacgg tcggcaagtg ccgcggcaaa tacgggccga gctcggcgaa gcgctcgaac
      781 cgcacctcga ggtcgcgaag atcgcggcag tttacgccgg ctaggagcgg cgagccgccg
      841 gctctcggcg cgtcgtaggc cgagagccgc tcgagatcgg cgcggtcgaa cgcctcgagc
      901 agcacgaaca tgcttaggcc ccgcgcgcaa tcgacgagcg cgcgcacggc cgcgtcatcg
      961 agcatcgcga ggatcacgag cacgccgccg gcgcccgccg cgcgcgcctc gagcacttga
     1021 taaggatcga cgagaaagtc cttgcgcatg acgggccgct taggccgtaa cgcctcggcg
     1081 gcagcggcga ggtgctcgag ctcgccgcgg aactcggtcg gctccgtaag cacggagacg
     1141 gccgcggcgc cgccgcgcgc gtaacccgcg agctgcgcgc gcgagtcgaa gtcgccgcgg
     1201 gcgaggccgc ccgcggacgg cgagcgcagc ttgagctcgg cgatgacgtc gaagcggtcg
     1261 agcccaagcg gcggcgcggc cggcgcgtcg agcgcgcggc gctcgagctt tgcctcgctc
     1321 tccttcgcgc gcgccgcgcg cacgcgttcg cggctcgcct tggccatgcg ctcgagaaac
     1381 gtcacggctt ctccttcgcg aacgcggcga tgcggtcgag tagcctcgtc ccggcgccgc
     1441 tcgcgagcga cttccgcgcc cgcgccacgg cgcttctagc gtcgggctcc tcgcccgtga
     1501 cctcgagcgc cagcgcggcg ttcagcacca gcgcgtcgcg gtgtgcggcc gtatcgcggc
     1561 cggcgagcgc ttcgcgcagg cgcgccgcgt tgccggccgc atcggtgccg cgcagctcgt
     1621 cgagcgtgca gcgttcgatg ccggcatcgc gcggctcgcg cagcgtgcgc tcgacgcggc
     1681 ccgggcgcac gtcgtagcag acgaatgggc cggcaggcgt cgcctcgtcc cagccggctt
     1741 cgccgtggac tacgaacgcg cgcgtcagcg gcaagccggc cagcgtgtcg gccatgctgg
     1801 ccgccattgc gtcgctgaat gcgccgatga cgtggaatgg cggcgcggcc ggattcgcga
     1861 gcggccccaa gatgttgaac acggtgcgca cgccgagcgc ttgacgcacc gcggcgatgc
     1921 ttttcgtcgc cgggtgatag tgcggcgcga acaggaacgt gaagccgcag cgctcgaagc
     1981 acgcgccggc tcgagcctcg tcgagcggga tcggcagccc gagcgcggcg agcacgtcgg
     2041 cgctgccgct cttgctcgag accgagcggt taccgtgctt gacgaccggc aagccgcacg
     2101 cggccgcgag cagcgaagcg cccgtcgaca ggttcaggct gcccgagccg tcgccgccgg
     2161 tgccgacgat gtcgacgtac gcgccggccg gaatcgcggg gcgcagcgcg agctcgcgca
     2221 tcgcgcgcgc gaagccgcgg atctcctcgg gcgtttcgcc cttcgcgcgc agcgccgcga
     2281 gcagcgctcc ggcgagggcc ggttgaattt cgccgcgcgc gagcaggcgc aacagctcgc
     2341 ccgccgtcgc ttcgtcgagg tgctcgccgc gcagcaagcg ctcgagcaat ttcggcgcct
     2401 cggcgctcat gggtgcagga aattcttcag caggtgcggc ccttcgggcg tcagaacgct
     2461 ctcgggatgg aactgcacgc cctcgacggc gagcttgcga tgccgaacac ccatgatctc
     2521 accgtccgtc gtgcgtgccg tcacgacgag atcgggcggc atcttctcgc ggttcgcggc
     2581 cagcgagtga tagcggccga cctcgaacgg attggacaag ccgcggtaga tcgtcgcgcc
     2641 gtcgtgctgc acgaccgagg tcttgccgtg catcagcgcc gccgcgccga tgatctcgcc
     2701 gccgaagtgc tgcacgatgc tctggtgccc caagcacacg ccgagcacgg gaatcttgcc
     2761 cgcgaacgcg ccgatcatcg cgagcgagct gccggcgtcg tcggggcggc ccgggcccgg
     2821 cgagatcacg agatgcgtcg gcttcagctt cgccgcgtcg gccaccgaca gcgcgtcgtt
     2881 gcggtagacg agcacctcgg cgtcgagcac gaggaacgcc tgcacgaggt tgtaagtaaa
     2941 ggagtcgtaa ttgtcgatca gcaagaggcg cacgctcata gtccctcctc ggcaagctcg
     3001 agggctcggc gcaggatcgc gctcttcgcc agcacttctt gatattccat ctccggcgag
     3061 ctgtcggcga cgatgccgcc gccggcctgg aagctgtact cgtcgccgtt gaacaccatc
     3121 gtgcgaatcg tgatcgcctg atccatgttg ccgttctttg cgaaatagcc gacggtgccg
     3181 ccgtagagcc cgcgccccgt cggttcgagc tcggcgatga gctgcatcgc tcgaaccttc
     3241 ggcgcgccga cgagcgtgcc cgcggggaac gcggccgcga acaggtcgaa ggcgtcgcgg
     3301 tcgggcgcga ccagcccctg cacgccgctg acgatgtgca tgacgtggct gtagcgctcg
     3361 atcttgcggt acggctcgac gtgcacggag cccgagctcg cgacgcgtcc caagtcgttg
     3421 cgcgcgaggt cgacgagcat gacgtgctcg gcgttctcct tcgggtccgc gagcagctcg
     3481 cgctcgagcg caaggtcgtg ctcgggcgtg agcccgcgcg gccgcgtgcc ggcgatcggc
     3541 ctgagcgacg ccgttcgtcc ggtgaggcgc acgagcgcct cgggcgagga gccggcgacg
     3601 cgcagcttgc ccagctcgag gaagtacatg tacggcgacg gattcaacaa gcgcaagccg
     3661 cgataggtct ggaacgcgtc gagcgcggtg cggcccgcga accgcaccga caacacgatc
     3721 tgatagatgt cgccggcctt gatgtgggct tttcctttca gcaccgacgc ggcgtgctct
     3781 tgctgcgtca tgctcgccgc ggccggcgag aacttgccgg gccggtccgg cgcgtcgagg
     3841 ccgccgcgca aagctttgac gacggccgtg cgcagcgcct ggcgctcgcg ctcgtcgccg
     3901 gcggagagca gcgcgacgcg ccgcgtcagg tgatcgaaca cgagcagcga ctccggcgcg
     3961 acatacgcgg cttgcgggag cccgtcgcgc tcggcgcgat tcggcggcaa gcgctcgaag
     4021 aagcgcacga cgtcgtagcc ggtcgtgccg acgagcccgc cgtcgaacgg gatgccctcg
     4081 atctgcggct tcaagagcgg cgcgcgctcg agcgccgtcc gcatcatcgt ttgccattcg
     4141 gccgcgttcg tgggccgccg cgcggccttg ccgtctatga gcagccggtc ggagtgcagc
     4201 tcgacggtca cggcctcgcc caaaccgatg aacgagtagc gcgcgaggcg ctcgccgctc
     4261 tcgacgctct cgagcaagaa gcgcggcttg aacggcttca gcttcatgta cgccgacacc
     4321 ggggtatcga gatcggcggc gatgtcgaac ggtgactcca tggctctgcc ttagttctta
     4381 tgctcccaga aacaaaaaac ccatctccgg tactcgcgaa gatgggtctg catgggtcga
     4441 gagaggacga gagtcttcta cgcgcgcact acccgcttcg ccggtgagac tcgccaccag
     4501 cggccatcgt aggcaggaac agttggccgt ccgctcatgg ccgggcagta tgtccgcggg
     4561 gctccctgcc ggtcaagccg gccagtccgc aaatttgtcg taaagtcgcg gcaatgagct
     4621 cgctaccggg tagacacgcg ccgctcgctc gcgcgggcgc cgagaaacgc tccggccgtc
     4681 aagcgctcga cgactacttg agcttctggc ggtacagccg ccgcgcaatc acgctcgtct
     4741 ggtcgacgca tcgagggctg acgatcgcgc tcggcctgtt gacgatcgcg gccggcctca
     4801 tgccggcggg catcgcctac gtcggcaagc tcatcgtcga cgccgtcgtc gtcgcgatcg
     4861 acttgaaccg cgccggcgcc gcgcccgact acgcgcgcgt gctcgagctc gtcgcgctcg
     4921 aaggcgtgct ggtcgcggcg ttgtccggcg cgcagcgcgg catcgggttc tgccagtcgc
     4981 tgctgcgcgt gctgctgtcg cagcgcgtga atttgctgat tctcgacaag gcgctgacgc
     5041 tgacgctcgc gcagttcgag gactcggagc tctacgacaa gctgaaccgc gcgcggcaag
     5101 aagcgtccgt tcggccgctg tcgctcgtga atcgcacgtt cgcgctcgcg cagaacttgg
     5161 tctcgctcgc gagctacgcg ggcttgctgc tcgcgttctc gccgtgggcc gtcgcgatcc
     5221 tcgtcgccgc gggcttgccg gccttcatcg ccgagacgcg cttctcgggc gagcgctttc
     5281 gcgcgttcca gtggcgcagc caagaccgcc gcatgctcgg ctacctcgag atcgtcatcg
     5341 cgcgcgagga tcacgcgaag gaggtcaagc tgttcgacct cggcgcgcgc ttgctcgcgc
     5401 gctttcgcga catcttccgc cgcatctatg aagaggagaa gcggctcacg ctgcgtcgcg
     5461 acggctgggg cttcgtgctc ggcctcgtcg cgagcgcggc gttctacggc gcgtacgtct
     5521 ggatcgcgct cgcgacgatc gacggcacga tctcgctcgg cgacatgacg atgtacttgc
     5581 tgctgttccg gcagggccaa tcggcagtcg cggcgatgct gagcgcgatc ggcggcatgt
     5641 acgaggacaa cctgtatctg tcgaacctct acgattatct cgagcagccg tcggcgggga
     5701 tccacggcac ggcgacgacg gggctcaacc cgcaggccgg catcgagttc gacgacgtga
     5761 cgttccaata tccgggcgcc acgaacgcgg cgctgcgcga cgtcacgctg caggtgcggc
     5821 cgggccagag cgtcgcgctc gtcggctcga acggctcggg caagacgacg ctcgtgaagc
     5881 tgctcgcggg cctttacacg cctacgcgcg gggtcgtgcg ctaccagggc cgcgatctgc
     5941 gcgactgg
//
LOCUS       DASSNN010000280         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_557005, whole genome shotgun sequence.
ACCESSION   DASSNN010000280 DASSNN010000000
VERSION     DASSNN010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557005"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /gene="gspK"
                     /locus_tag="VFL84_05080"
     CDS             <1..795
                     /gene="gspK"
                     /locus_tag="VFL84_05080"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HET7608028.1"
                     /translation="DQGLMYVQGAEAWAADILRQDLVDSPDSDNLGEVWATELPPLPV
                     DDNGGTIMGRLEDLQGRFNLNNLIGADGKENELMRRQFERLLVSVEADPALAGAVIDW
                     LDPDTEQRFPTGGEDVVYSDAEPPYRTANSMITSTSELMAVAGFDRDIYRRLAPYITV
                     LPAGTKINVNTASDVVLASLSDNIDLSTAASLIEQRGDADFVDIDMTFEGLLEPDMLQ
                     EIDGVSEHFLLTATVTLGSNQLTMRSVLQRDRSGITRAVFRSLGVE"
     gene            797..2020
                     /gene="gspL"
                     /locus_tag="VFL84_05085"
     CDS             797..2020
                     /gene="gspL"
                     /locus_tag="VFL84_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HET7608029.1"
                     /translation="MAETFFVRWSDAGTATWGAFDQTGRLVGSLGSGDLQAAQAAQAG
                     RRCTVLVNAIDVLTAEASLPAASQSRLRQIAPFSLEELLANDVDEMVFAIGPRLPSGA
                     TLVAAVAKERMDAWLDELRAAGIVPNALVSEADGIPDMPATLVLVIEGDRVLGRKPGR
                     APFVFEGFDLAHVLPLVLARKPDEPELHHVRIFTDAAGHERFAEELATLGGRFASADV
                     KILNDGVFAYLAATLAQRPGTNLLQGAYAPKSNWRAMAKPWRVAASLAGAAVVLALLL
                     QGAQLWQLRRADTALDAVLGPECQRVVGDSSMSGCQREVAQRLGTRADNATEDFLSTL
                     AAVAAALDANTRVDALNYRNRAMNLQLVTQGTDAAVAFERKLEQSRRFAVELESTNPT
                     DSGTEVRLRVVGANP"
     gene            2017..2496
                     /locus_tag="VFL84_05090"
     CDS             2017..2496
                     /locus_tag="VFL84_05090"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HET7608030.1"
                     /translation="MKGALLAWFYGLQPRERWLVAIGAALFVVIVGWGFILKPLNADV
                     ARLQESVQTKQRLLVDVARVEVERPQTVADGRQGNDRALVQLIPDTARDFGLQEPRSR
                     ANGPSGVDVTLQNASYDAIEEWLVALHQPYGVDVETATFTPAREPGYVNGSVLLRRP"
BASE COUNT          401 a          835 c          861 g          400 t
ORIGIN      
        1 gatcaggggc tgatgtacgt gcaaggcgcg gaagcttggg ccgccgacat cctgcgtcag
       61 gatctcgtcg attctccaga cagcgacaac ctcggcgaag tctgggcgac cgagctgccg
      121 cctttgccgg tcgacgacaa cggcggaacg atcatgggcc ggctcgagga tctgcagggc
      181 cgcttcaatc tcaacaatct gatcggcgcg gacggcaagg agaacgagct catgcgccgg
      241 cagttcgagc gcctgctcgt cagcgtcgaa gcggacccgg cgctcgccgg cgccgtcatc
      301 gactggctcg accccgatac cgagcagcgt tttccgaccg gcggcgagga cgtcgtctac
      361 agcgacgccg agccgccgta tcgaacggca aactcgatga tcacgtcgac gtcggagctc
      421 atggccgtcg cgggcttcga ccgcgacatc tatcggcgcc tggcgccgta catcaccgtg
      481 ttgcccgcgg gaacgaagat caacgtcaac accgcgtccg acgtcgtgct cgcgtcgctg
      541 tcggacaaca tcgacctctc gacggcggct tctttgatcg agcagcgcgg cgacgccgat
      601 ttcgtcgaca tcgacatgac gttcgagggg ctgctcgagc ccgacatgct tcaggagatc
      661 gacggcgtca gcgagcactt cttgttgacc gcgacggtga cgctcgggtc aaatcagctc
      721 acgatgcgtt ccgtgctgca gcgcgatcga agcggcatca cgcgggccgt gttccgcagc
      781 ctcggagtcg aatagcttgg ctgagacgtt cttcgttcgt tggtccgatg cgggaacggc
      841 gacctggggc gcattcgacc agacggggcg cctcgtcggc tcgctcggca gcggcgactt
      901 gcaggccgcg caagcggcgc aggcgggccg gcgctgcacc gtgctcgtga acgccatcga
      961 cgtgctgacg gcggaagcgt ccctacccgc ggcaagccag tcgcggctcc ggcaaatcgc
     1021 gccgttctcg ctcgaggagc tgctcgcgaa cgacgtcgac gagatggtgt tcgcgatcgg
     1081 tcccaggctg ccgtccggcg caacgctcgt cgcggcggtc gcgaaggagc gcatggacgc
     1141 gtggctcgac gagctccgtg ccgcgggaat cgtgccgaat gccctggtct ccgaggcgga
     1201 cggaataccg gacatgccgg caacgctcgt gctcgtcatc gaaggcgacc gcgtcttggg
     1261 gcgcaagccg ggccgcgcgc cgttcgtgtt cgagggtttc gacctcgcgc acgtattgcc
     1321 gctggtgctc gcgcgcaagc ccgacgagcc cgagctgcat cacgtccgaa tcttcacgga
     1381 cgcagcgggc cacgaacgct tcgccgagga gctggccacg ctcggcggcc ggttcgcgag
     1441 cgccgacgtc aagatcttga acgacggcgt cttcgcgtac ctcgccgcga cgctcgcgca
     1501 gcggcccggc acgaacctgc tgcaaggcgc gtacgcgccg aagtcgaact ggcgcgcgat
     1561 ggcgaagccg tggcgggtcg cggcgtctct cgcgggcgcg gccgtcgtgc tcgccttgct
     1621 gttgcaaggc gcgcagctct ggcagctgcg ccgcgcggat acggcgctcg acgccgtgct
     1681 ggggccggaa tgtcaacgcg tcgtcggcga ctcgagcatg tcgggttgtc agcgcgaagt
     1741 cgctcagcgg ctcggcacgc gagccgacaa cgcgaccgag gatttcctga gcacgctcgc
     1801 cgccgtcgcg gccgcactcg acgcgaacac gcgcgtcgat gcgctgaact atcgcaatcg
     1861 cgccatgaat ctgcagctcg tcacgcaggg caccgatgca gcggtcgcgt tcgagcgcaa
     1921 gctcgagcaa tcgcgccgct ttgccgtcga gctcgagtcg acgaatccga ccgactcggg
     1981 caccgaggtt cgcttgcgcg tcgtcggagc gaatccatga aaggcgcgct cttggcgtgg
     2041 ttctacggcc tgcaaccgcg cgagcggtgg ctcgtcgcga tcggcgccgc gctgttcgtc
     2101 gtcatcgtcg gctggggttt catcttgaaa ccgttgaatg ccgatgtcgc gaggttgcag
     2161 gagtccgtgc agacgaagca gcggctgctc gtcgacgtcg cgcgcgtcga ggtcgagcgc
     2221 ccacagacgg tcgcggacgg ccggcagggc aacgatcgag cgctcgttca gctgatcccg
     2281 gacacggccc gcgacttcgg cctccaggag ccgcggtcgc gggccaacgg cccgagcggc
     2341 gtcgacgtca cgctgcaaaa cgcgtcgtac gatgcgatcg aagagtggct cgtcgcgctg
     2401 caccagccgt acggcgtcga cgtcgagacc gccacgttca cgcccgcgcg cgagccgggc
     2461 tacgtgaacg gctcggtgtt gctgcgcagg ccctgaa
//
LOCUS       DASSNN010000281         3040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_794667, whole genome shotgun sequence.
ACCESSION   DASSNN010000281 DASSNN010000000
VERSION     DASSNN010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794667"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFL84_05095"
     CDS             <1..255
                     /locus_tag="VFL84_05095"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET7608031.1"
                     /translation="QRTGESTFVVAGELTLLGKRRPLTLEATLNKSAPYPFGDRAEVM
                     GVSARAKLKRSEFGMTYGVADDLVGDDVEIVIEFEARKAP"
     gene            276..1349
                     /locus_tag="VFL84_05100"
     CDS             276..1349
                     /locus_tag="VFL84_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HET7608032.1"
                     /translation="MTRALAVGVAIALMVARTAAAQVDSPAEDAAFQTFLGEIRASAA
                     ERGIRAETLDAVLPAIRIRREAVEADRSQAEFVLTYEAYLRRVSPARIARGRAFNREH
                     GEMIRRVAADYGVQPRFVVAILGLESDYGTYPIEQPLFDVVATLAFDGRRGAQFRTQL
                     LAALEIVDKGWATPEEMKSSWAGALGMPQFTPTNVLDIAVDHDRDGRVDLWKIGPDVV
                     ASVANYLRRNGWRGDLTWGREVKLPAGGEQTLAAPMAEGATPPAVCRSYESLGAFRDL
                     GDWQALGVRRSGGGSLPTRKLPAALIVGDEGDDRGWLVYQNFCSLMRYNPAFRYALSV
                     GLLADAIGDVNGVAAPATRTPSN"
     gene            1373..2656
                     /locus_tag="VFL84_05105"
     CDS             1373..2656
                     /locus_tag="VFL84_05105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7608033.1"
                     /translation="MEPRPETPLPKRRWTRAQVVYAVALFVSAWTLVGLALARSDAVL
                     SDGGELAAAAFFFAFGLFTISMGYQHPNLGYYSFDRVSQVASILVLGPVAAALINGLA
                     SFVYPWHRLRKGVPPREVLYAAINNSGLMAAIVLAAGLAYSAVGGPVPLTSLTPPAIA
                     ALLVLVLGMQVLNDAGMLVLYVLGRRSLAGFFHAFSYALELGAGATAVLVAIIFNTMG
                     LEVFLLMLVVLSVGMLALRQFADMRHKLELIVAERTQKLEEKSRELEVQATRDNLTGL
                     FNRRYADEYLAHEMESCRRERRPLTVAFADIDFFKRVNDLHSHAMGDAVLRRVADVLR
                     DRCRANDVLARYGGEEFLLCFPNTDLREARLVCEELRTAVERSNWAQLGLAGGVTISF
                     GIADYRTDASAERLLNRADVRLYEAKHGGRNLVVA"
     gene            complement(2842..>3040)
                     /locus_tag="VFL84_05110"
     CDS             complement(2842..>3040)
                     /locus_tag="VFL84_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HET7608034.1"
                     /translation="GRRVVLPYRWVYCLLIPLGAVLAPELVWAWGDLMNALQIFPNVI
                     GLIGLSAIAAKYAANRGHRAA"
BASE COUNT          471 a         1052 c         1036 g          481 t
ORIGIN      
        1 cagcgcacgg gcgagagcac gttcgtcgtc gccggcgagc tcacgctgct cggcaagcgc
       61 aggccgctga cgctcgaggc gacgctgaac aagagcgccc cctacccgtt cggcgatcgt
      121 gcagaggtaa tgggcgtctc ggcgcgcgcg aagttgaagc gcagcgagtt cggcatgaca
      181 tacggcgtcg cggacgatct cgtgggcgac gacgtcgaga tcgtcatcga attcgaggcg
      241 cgcaaagcgc catgagcgac acgtcgagtc tctccatgac gagagcgctc gcggtgggcg
      301 tcgcgattgc actaatggtc gcccgcaccg cagcggcgca ggtcgattct ccggccgaag
      361 acgccgcgtt ccagacgttt ctgggcgaga tccgcgcttc ggccgccgag cgcggcattc
      421 gcgccgagac gctcgacgcc gtgctgccgg cgatccgcat tcgccgcgaa gccgtcgagg
      481 ccgaccgctc gcaggccgag ttcgtgctga cgtacgaggc gtatctgcgc cgcgtgagcc
      541 ccgcgcgcat cgcgcgagga cgcgccttca acagagagca cggcgagatg atccgccgcg
      601 tcgccgcgga ctacggcgtg cagccgcgct tcgtcgtcgc gatcctgggg ctcgagagcg
      661 actacggcac gtatccgatc gagcagccgc tgttcgacgt cgtcgcgacg ctggcattcg
      721 acggccggcg cggcgcgcaa ttccgcacgc agctcttggc cgcgctcgag atcgtcgaca
      781 agggctgggc tacgcccgag gagatgaaga gctcgtgggc aggcgcgctc ggcatgcccc
      841 agttcacgcc gacgaacgtg ctcgacatcg ccgtcgatca cgaccgcgac ggccgcgtcg
      901 acctctggaa gatcgggccc gacgtcgtcg catccgtcgc gaattatttg cggcggaacg
      961 gctggcgcgg agatctgacc tggggccgcg aagtgaagct gcccgcgggc ggcgaacaga
     1021 cgctcgcggc gccgatggcc gaaggcgcga ccccgccagc ggtttgccgc agctacgaaa
     1081 gcttgggcgc gttccgcgac ctcggcgact ggcaagcgct cggcgtgcgg cgcagcggcg
     1141 gcgggagtct gcccacgcgc aagctgcccg ccgcgctcat cgtcggcgac gaaggcgacg
     1201 accgcggctg gctcgtctac cagaactttt gctcgttgat gcgctacaac ccggcattcc
     1261 gctacgcgct gtcggtgggc ttgctcgccg acgccatcgg cgacgtgaac ggcgtcgcag
     1321 cgccggcgac gcgcacgccg agcaactgag ctgctgtgtt atcgtccgcg gcatggagcc
     1381 acggccggaa acgccgctgc ctaagcgacg ctggacccgc gcgcaggtcg tctatgccgt
     1441 tgcgctgttc gtttccgcat ggacgctcgt cggcctcgcg ctcgcgcgca gcgacgccgt
     1501 gctcagcgac ggcggagagc tcgccgcggc ggcgttcttc ttcgcattcg gcctgttcac
     1561 gatcagcatg gggtaccagc acccgaacct cgggtactac tcgttcgatc gcgtctcgca
     1621 ggtcgcgagc atcctcgtgc tcggccccgt ggcggccgcg ctgatcaacg gcctcgcgtc
     1681 gttcgtctac ccgtggcacc gcctgcgcaa aggcgtcccg ccgcgcgagg tcttgtacgc
     1741 cgcgatcaac aactcgggcc tcatggccgc gatcgtcctc gcggcgggcc tggcctattc
     1801 ggccgtcggc ggcccggtgc cgctcacgag cctcacgccg cccgcgatcg cggcgctgct
     1861 cgtgctcgtg ctcggcatgc aggtcttgaa cgacgccggc atgctcgtgc tctacgtgct
     1921 cggccggcgc agcctcgccg gattcttcca cgcgttctcg tacgcactcg agctcggcgc
     1981 cggcgcgacg gccgtgctcg tcgcgatcat cttcaacacg atggggctcg aggtgtttct
     2041 gctgatgctc gtggtcttga gcgtcggcat gctcgcgctg cggcaattcg ccgacatgcg
     2101 ccacaagctc gagctcatcg tggccgagcg cacgcagaag ctcgaggaga agtcgcgcga
     2161 gctcgaagtg caggcgacgc gcgacaacct gacggggctg ttcaatcggc gctacgccga
     2221 cgagtatctg gcgcacgaga tggagagttg ccggcgcgag cgccggccgt tgacggtcgc
     2281 gtttgccgac atcgacttct tcaagcgcgt caacgatctt cattcgcacg cgatgggcga
     2341 cgcggtgttg cggcgcgtgg ccgacgtgct gcgcgaccgc tgccgcgcga acgacgtgct
     2401 cgcgcgctac ggaggcgagg agttcctgct ctgcttcccg aacacggatt tgcgcgaggc
     2461 gcgcctcgtc tgcgaggagc tgcggaccgc cgtcgagcgc agcaactggg cgcagctcgg
     2521 cctcgccggc ggcgtgacga tcagcttcgg gatcgccgac tatcggaccg acgcttccgc
     2581 cgagcggctg ctgaaccgcg ccgacgtgcg gctatacgaa gcgaagcacg gcggccgcaa
     2641 cctcgtcgtc gcgtagcgcg ccggatgctc ggccgccgcg tcgcggcccg cgcgcgccgc
     2701 gagaagccac cgcttccaga ccatctcgac gagatcgtcg agctcatcca acgcctgaaa
     2761 cagagtctca atcgccgtcg accgtccttg gtgcccttac tgctcaaaac ggttgagggc
     2821 gccaaacggt tcccttcaaa gctatgcggc tcgatgaccg cggttcgcgg cgtacttcgc
     2881 ggcaatcgcg ctcaaaccga tgagcccgat cacgttcggg aagatctgca gcgcgttcat
     2941 gagatcgccc caggcccaca cgagctcggg agcgagcacg gcgccgagcg gaatcagcaa
     3001 gcagtagacc cagcggtacg gcagcacgac gcggcggccg
//
LOCUS       DASSNN010000282         3525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_288096, whole genome shotgun sequence.
ACCESSION   DASSNN010000282 DASSNN010000000
VERSION     DASSNN010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288096"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VFL84_05115"
     CDS             <1..942
                     /locus_tag="VFL84_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7608035.1"
                     /translation="ESNNLALQGVLRRSRNRRVHLVTSRIEHKSVLDVARALESSGVA
                     VTYVPATPGGWVDPQRVVDALDDDTQLVSIMHVNNETGVIQDVGAIGEACRARGVLFH
                     VDAAQSAGKLPLALREAPIDLCSLTAHKLCGPKGVGALYLAPRVTLVPQLLGGEQERA
                     LRAGTVATHQVVGMGKAYELAEPEREAPRLAALRDRLWRALAGIEGARANGDPERRAP
                     HVLNVTFPGVDGESLRFALHDVCVSAGSACAADSPEASHVLTGMGLSDVQAASSLRFS
                     VGRFTTEDDVVTAAARVAAAVARLRSLARSAPAWCSA"
     gene            971..1345
                     /locus_tag="VFL84_05120"
     CDS             971..1345
                     /locus_tag="VFL84_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HET7608036.1"
                     /translation="MEYSAAVERHFAAPKIARELPAGSPGLVAGEAEDRTLHVWVRFQ
                     LQIVDGVVAAAGFRAFGCPHTVASASAVAEWLEGRPVEEARKLDVKAICAELDVPVEK
                     LGKLLRIEDAVAACWRTVSEQR"
     gene            1353..1676
                     /gene="iscA"
                     /locus_tag="VFL84_05125"
     CDS             1353..1676
                     /gene="iscA"
                     /locus_tag="VFL84_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011147476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HET7608037.1"
                     /translation="MSISLTQSAATRVRTYLEKRGSGVGLRVGVRRSGCNGWAYTIDY
                     ADSVAEGDVVFDQQGVKVVVDARSLELIDGTTVDFIKEGLNEAFKFRNPNVKGECGCG
                     ESFSV"
     gene            1711..2142
                     /gene="ndk"
                     /locus_tag="VFL84_05130"
     CDS             1711..2142
                     /gene="ndk"
                     /locus_tag="VFL84_05130"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET7608038.1"
                     /translation="MAVERTLSIVKPDGVAKNLIGEVYRRFEKGGLKIIAAKMLRLRQ
                     DQAEGFYAVHKARPFYADLVRYMTSGPVMVQVLEGDNAIQLNRDIMGATNPKQAAPGT
                     IRADFAASIEENVVHGSDGPETAKNEIAFFFSEAELCPRTR"
     gene            2124..3233
                     /gene="rlmN"
                     /locus_tag="VFL84_05135"
     CDS             2124..3233
                     /gene="rlmN"
                     /locus_tag="VFL84_05135"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET7608039.1"
                     /translation="MPANALALRATDKPSKLSLLGLPAAELVELFENIDEKPYRARQI
                     MRWLYQRYVTDYAEMTDLSAALRKQLAELTTLALPPVLKHEHSDDGTRKWLLDVGANQ
                     AIETVYIPEPDRGTLCISSQAGCALDCAFCATGHQGFNRNLTSAEILGQVVLAARELA
                     PAKITNVVFMGMGEPLANYRNVVRVAHLLLDDQAFGLSRRRVTISTAGVVPQMLKLAS
                     ECNVALAVSLHAPTDELRDKLVPINKVHPIAELLDACWKYALGQASRSITFEYVMLDG
                     VNDSVAQARQLVKLLRNRPAKINLIPFNAFPESEFRCSSKGAIDAFWQTLRNAGLIAT
                     VRRPRGDDIAAACGQLAGRVRDRQRVRLGDRVIGA"
     gene            3238..>3525
                     /locus_tag="VFL84_05140"
     CDS             3238..>3525
                     /locus_tag="VFL84_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7608040.1"
                     /translation="MNTLRSACLIAMLAASTACVSSGGSVSDPASGNEAAIANMNLGA
                     GYLRQGNTTLALERLQRALAQDPNLVQAHTTIAIVYDQIGNLEEAENHFKRA"
BASE COUNT          592 a         1137 c         1213 g          583 t
ORIGIN      
        1 gagtcgaaca atttggcgct gcaaggcgtg ctgcgccgct cgcgcaaccg ccgcgtgcat
       61 ctcgtgacct cacgcatcga gcacaagtcc gtgctcgacg tggcacgtgc actcgaatcg
      121 agcggagtgg cagtcaccta cgttccggcg acgccgggcg gctgggtcga tccgcagcgc
      181 gtcgtcgatg cgctcgatga cgacacgcag ctcgtctcga tcatgcacgt caacaacgag
      241 acgggggtca tccaagacgt cggggcgatc ggcgaagcct gccgggcgcg cggcgtgctg
      301 ttccacgtcg acgcggccca gagcgccggc aagctgccgc tagcgcttcg cgaggccccg
      361 atcgacctct gctcgctgac ggcgcacaag ctgtgcggcc cgaaaggcgt gggcgctctc
      421 tatctggcgc cgcgcgtgac gctcgtgccg cagctgctcg gcggcgagca ggagcgcgca
      481 ttgcgcgccg gcacggtcgc gacgcatcaa gtcgtcggca tgggcaaggc ctacgagctc
      541 gccgagcctg agcgcgaggc gccgcgcctg gccgcgctgc gcgatcggct ctggcgcgcg
      601 ctcgccggca tcgagggcgc ccgtgcaaac ggcgaccccg agcgccgggc gccgcacgtc
      661 ttgaacgtaa cgttcccggg cgtcgacggc gagagcctgc gctttgcgct gcacgatgtc
      721 tgcgtctcgg cggggtcggc ttgcgccgcg gacagccccg aagcctcgca cgtcctgacc
      781 ggcatgggct tgagcgacgt ccaggccgcc agctcgctgc gtttcagcgt cggtcggttc
      841 acgaccgagg acgacgtcgt gacggcggcc gcgcgcgtcg ccgcggccgt cgcgaggctg
      901 cgctcgctgg cccgctcggc gccggcgtgg tgctcggcct gacttttttc ggctaaacta
      961 ttgacccgcc atggaataca gcgcggccgt cgagcggcac ttcgcggcgc cgaaaatcgc
     1021 gcgggaactg ccggccggca gcccgggtct cgtggcgggc gaggccgagg accgcacgct
     1081 gcacgtctgg gtgcgcttcc agctgcaaat cgtcgacggt gtcgttgcgg cggcagggtt
     1141 tcgagcgttc ggttgtccgc acacggtggc ctcggcgagc gcggtggccg agtggctcga
     1201 gggccggccg gtcgaggaag cgcgcaagct cgacgtgaaa gcgatctgcg ccgagctcga
     1261 cgtgcccgtc gagaagctcg gcaagctgct gcggatcgag gatgcggtcg cggcgtgttg
     1321 gcgcacagtc agtgaacaaa ggtgagcacg gaatgtcgat ttcgttgacg caaagtgcgg
     1381 cgacccgcgt ccgcacgtat ctcgagaagc gcggtagcgg tgtggggctc cgggtcggag
     1441 tgcggcgtag cggctgcaac ggctgggcct atacgatcga ctacgccgat agcgtggccg
     1501 agggcgacgt cgtcttcgat cagcaaggcg tcaaggtcgt cgtcgacgcg cggagcttgg
     1561 agctcatcga cggcaccacc gtggacttca tcaaggaagg tctcaacgag gcgttcaagt
     1621 tccgcaaccc gaacgtcaag ggcgaatgcg gctgcggcga aagcttcagc gtctaggcgt
     1681 atttcttctg gtcaactctc aaggtgaaca atggctgtcg agcgtacgtt gtccatcgtc
     1741 aaacccgacg gtgtggcgaa gaatctgatt ggtgaggtct accgccggtt cgagaagggc
     1801 gggctcaaga tcatcgcggc aaaaatgctg cggttgcgtc aggatcaggc cgaaggattc
     1861 tatgcagttc acaaggcgcg cccgttctat gcggatctcg tgcgctacat gacgtcgggg
     1921 cccgtgatgg tgcaggtgct cgagggcgac aacgcgatcc agttgaaccg ggacatcatg
     1981 ggcgccacga acccgaagca ggcggcgccc ggcacgattc gcgccgactt cgccgcctcg
     2041 atcgaggaga acgtcgtgca cggctcggac ggtccagaga cggccaagaa cgagatcgcg
     2101 ttcttcttca gcgaggccga gctttgcccg cgaacgcgct agcgctgcga gccacggaca
     2161 agccgagcaa gctgtcgctg ctcggcttgc ccgcggccga gctcgtcgag ctcttcgaga
     2221 acatcgacga gaagccctat cgcgcgcggc agatcatgcg ttggctctac cagcgctacg
     2281 tcaccgacta cgcggagatg acggacttga gcgcggcgct gcgcaagcag cttgccgagc
     2341 tgacgacgct cgcgctgcct cccgtcttga agcacgagca ttccgacgac ggcacgcgca
     2401 agtggctgct cgacgtcggc gcgaatcagg cgatcgagac cgtgtacatc ccggagccgg
     2461 atcgcgggac cttgtgcatc tcgtcgcagg ccggctgcgc gctcgactgc gcgttctgcg
     2521 cgacgggcca tcagggcttc aaccgcaatc tgacgtcggc cgagatcctg gggcaggtcg
     2581 tgctcgcggc ccgcgagctc gcgcctgcca agatcacgaa cgtcgtgttc atgggcatgg
     2641 gcgagcctct cgcgaactac cgcaacgtcg tgcgcgtcgc gcatctgctg ctcgacgatc
     2701 aggcgttcgg cttgtcgcgc cgccgcgtga cgatcagcac ggccggcgtc gtgccgcaga
     2761 tgctgaagct ggcctcggaa tgcaacgtcg cgctcgcagt atcgctgcat gcgccgaccg
     2821 acgagctgcg cgacaagctc gttccgatca acaaggtgca cccgatcgcc gagctgctcg
     2881 acgcgtgctg gaaatacgcc ttgggccaag cgagccgcag catcacgttc gagtacgtga
     2941 tgctcgacgg cgtcaacgat tccgtggcgc aggcgcgcca gctcgtgaag ctgctgcgca
     3001 accggccggc caaaatcaac ttgatcccgt tcaatgcgtt tcctgagtcc gagttccgct
     3061 gctcgtccaa aggcgcgatc gacgcgttct ggcagacgct gcgcaacgcc ggcttgattg
     3121 caacggttcg tcggccgcgc ggcgacgata tcgccgccgc gtgcgggcag cttgccggcc
     3181 gcgtgcgcga caggcagcga gtgcgtttgg gtgatcgtgt gatcggagct tgagtacatg
     3241 aacacacttc gttcggcgtg ccttattgcg atgctcgcgg cgtcgacggc ctgcgtctcg
     3301 tccggcggct cggtgagcga ccccgcctcg ggaaatgaag ccgcgatcgc gaacatgaat
     3361 ctaggcgccg gctacttgcg gcagggcaat accacgctcg cgctcgagcg tctgcaacgc
     3421 gctttggcgc aggacccgaa cctggtgcag gcgcacacga cgatcgcgat cgtatacgac
     3481 cagatcggca acctcgagga agccgagaat cacttcaagc gcgcc
//
LOCUS       DASSNN010000283         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_288708, whole genome shotgun sequence.
ACCESSION   DASSNN010000283 DASSNN010000000
VERSION     DASSNN010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288708"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1059
                     /locus_tag="VFL84_05145"
     CDS             19..1059
                     /locus_tag="VFL84_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HET7608041.1"
                     /translation="MELYLDSVDFKEIEAALDFGFVKGLTTTPTFMHRHGITDIDGAI
                     VKLSKMVPELQVEALGDTPDQIVAEAERLVGLGLDTSKTVFKVPISNDGVKACHRLRS
                     RGYKVNVHLIYTLNQAYMAMEAGASFICPLAGRMQDQGHDAIQLFEHCVDVIDRYGYN
                     SKVMFSSVRHAEHVRQAMLTGVHVCTMPFSVMKSLCENTLTALGTAQFREHTALMTQR
                     VKDVIRKANPVCETSETLASAMVKMTESRLGLVTLVDSKGALAGVFTDGDLRRKLQSD
                     GRAILDKTLAAIGFSKSPHTLDRESLLNEAVKLFKKAEVDSIVVVDGTRPVGVLDVQD
                     LIKLGLLGQEHL"
     gene            1062..1754
                     /locus_tag="VFL84_05150"
     CDS             1062..1754
                     /locus_tag="VFL84_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HET7608042.1"
                     /translation="MKPEAELAVFSRLGGAFVTPIATLVERMRTVRGFVSDWDGVFNQ
                     GTKGEGAESTYSEPDSMGTNLLRYALWRAHGQLPVAALITGAENPSARTFALREHFHA
                     VYFGARNKTVAIESLCRAHRISSDRLVCIFDDVNDLGMAFACGIRVFVQRTASPLLRE
                     YVVRQGLCDYVTAHSSERHAVREVCELLLGLLGTFEAVVASRVAWDASYAEYFAARQA
                     LATEFVDQRVND"
     gene            1756..>2159
                     /locus_tag="VFL84_05155"
     CDS             1756..>2159
                     /locus_tag="VFL84_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HET7608043.1"
                     /translation="MGVWRFAVYLLLPYAIGNLIWRALRYSAYWHRWPERFGFVTPLR
                     GMRTLWVHAVSVGEVRSAAPLVAALVERYPKHRIVVTTMTPTGSQQVRDLFGDSVSHC
                     YVPFDFPDAVRRFFDRVRPEAAVIAETEFWPN"
BASE COUNT          359 a          702 c          708 g          390 t
ORIGIN      
        1 gtgcactaga ggaacagtat ggagctctat ctcgactccg tagatttcaa ggaaatcgaa
       61 gccgcgctcg acttcggttt cgtcaaaggg ctcacgacga cgcccacgtt catgcaccgg
      121 cacggcatca ccgacatcga cggcgcgatc gtcaagctgt cgaagatggt gcccgagctg
      181 caagtcgagg cgctcggcga tacgccggat cagatcgtcg ccgaggccga acggttggtc
      241 gggctcgggc tcgacacgtc gaagaccgtg ttcaaggtgc caatcagcaa cgacggcgtg
      301 aaagcgtgtc atcgactgcg ttcgcgcggc tacaaggtca acgtgcacct gatctacacg
      361 ctgaaccagg cctacatggc catggaagcg ggagcttcgt tcatctgtcc gctggccggc
      421 cgcatgcagg accagggtca cgacgcgatc cagctgttcg agcactgcgt cgacgtcatc
      481 gaccggtacg gctacaacag taaggtgatg ttctcgtccg tccggcacgc cgagcacgtg
      541 cgccaggcga tgctgacggg cgtgcacgtc tgcacgatgc cgttctcggt catgaagagt
      601 ctctgcgaga acacgctgac ggcgctcggc acggcgcagt tccgcgagca cacggctctg
      661 atgacgcagc gcgtcaagga cgtcattcgc aaagcgaatc ccgtttgcga gacgagcgag
      721 acgctcgcgt ccgcgatggt caagatgacc gagtcgcggc tcggcctcgt cacgctcgtc
      781 gattcgaagg gcgcgctcgc cggcgtgttc accgacggcg acttgcgccg caagctgcag
      841 tccgacggcc gcgcgatcct cgacaagacg ctcgccgcga tcggtttctc gaagagtccg
      901 cacacgctcg accgcgagag cttgttgaac gaggccgtca agctgttcaa gaaggccgag
      961 gtcgacagca tcgtcgtcgt cgacggaacg cgtcccgtcg gcgtgctcga cgtgcaggat
     1021 ttgatcaagc tggggttgct cggccaagag cacctctgac gatgaagccc gaagccgagc
     1081 tcgcagtttt ctcgaggctc ggcggcgcct tcgtaacgcc gatcgccacg ctcgtcgagc
     1141 ggatgcggac cgttcgcggc ttcgtgtccg attgggacgg cgtcttcaat caaggcacga
     1201 agggcgaggg cgccgaaagc acgtacagcg agcccgactc gatgggcacg aacctgctgc
     1261 gctacgcgct gtggcgcgcg cacgggcagc tgccggtcgc ggctctgatc acgggcgccg
     1321 agaatccgtc cgcgcgcacg ttcgcgctgc gcgagcattt ccacgccgtc tacttcggcg
     1381 cgcgcaacaa gaccgtcgcg atcgagtcgt tgtgccgcgc gcaccgcata tcgtcggacc
     1441 ggctcgtctg cattttcgac gacgtcaacg acctcggcat ggcgttcgcg tgcggcattc
     1501 gtgtgttcgt gcagcgcacc gcgagcccgc tgctgcgcga gtacgtcgtg cgccaggggc
     1561 tgtgcgacta cgtgacggcg cactcgtccg agcggcacgc cgtgcgcgag gtctgcgagc
     1621 tgctgctcgg cttgctgggc acgttcgagg ccgtcgtcgc gtcgcgagtc gcgtgggacg
     1681 cgagctatgc cgagtacttc gcggcgcggc aagcgctcgc gacggaattc gtcgaccagc
     1741 gcgtgaacga ttgacgtggg cgtctggcgc ttcgccgtct atctgctgtt gccgtatgcg
     1801 atcggcaacc tgatctggcg cgcgctgcgc tattccgcgt actggcaccg ctggcccgag
     1861 cgtttcggct tcgtgacgcc gctgcgcggc atgcgcacgc tctgggttca tgccgtgtcc
     1921 gtcggcgagg tcagatccgc cgcgccgctc gtcgccgccc tcgtcgagcg gtatccgaag
     1981 catcgcatcg tcgtcaccac gatgacgccg accggctcgc agcaggtgcg cgacctgttc
     2041 ggcgacagcg tcagccactg ctacgtgcct ttcgattttc cggacgccgt gcgccgtttc
     2101 ttcgatcgcg tgcgtcccga agccgccgtg atcgccgaga ccgagttctg gccgaacat
//
LOCUS       DASSNN010000284         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1802243, whole genome shotgun sequence.
ACCESSION   DASSNN010000284 DASSNN010000000
VERSION     DASSNN010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802243"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1730)
                     /locus_tag="VFL84_05160"
     CDS             complement(<1..1730)
                     /locus_tag="VFL84_05160"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HET7608044.1"
                     /translation="MTFALAAEATELSLETKRLLWTVAIVVGASLPHWPTLSPWIPAF
                     LLASVAWRLGVAFFGWPPATRIVRLPLSLIVFLAVLFQYRTLNGVEAGSALLVVMIGL
                     KVLESRSQRDQLVLIMICYFLMFARLLADRGPLAAVYTVLLVWLATVALIQIGRRGVL
                     LPYRATGLFSGRLLLHAMPVMVVLFVLFPRLPGPIWAIPGSTSSGATGLNDTMSPGDI
                     TNLALSDEVAFRVQFDGRPPRARDLYWRGPSLTEFNGRTWSMSQGSMRRGERVAATIE
                     YRGEPTSYRVTLEPNGRNWAFALDMPREWSGASALRMGSDYQLGTPFGAPRSRRLDYR
                     VTSYVDYRAREPLNENERAMFRALPERSNPRARALAASWLADSPSGATIVDRAMGYLA
                     SQPFAYTLTPPPLGAQPVDEFLFETREGFCEHYASALTFLLRAAGLPARVVMGYQGGE
                     LNAVGGYYIVRQTDAHAWTEVWLEDEGWVRVDGVAAVAPERVALGSDGFGSGGVTAAA
                     AALRARWSRPIALLWDAVNTRWQSWVVGYGPELQRSLLESLGFTNLRRAQRSGVLLGL
                     AVVATLTLLIG"
     gene            complement(1727..2701)
                     /locus_tag="VFL84_05165"
     CDS             complement(1727..2701)
                     /locus_tag="VFL84_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7608045.1"
                     /translation="MRLDVAHSLERWAAVWARRRQGADAHTIALRRRRIYILPTRFGV
                     VFASMVFAMLLGSLNYGASLGFALTFLLTGLGLVVMHHCHNNLLGASIKFLGAAPVFA
                     GERAEFKIAVGNDAPAARLEIELKHKEHAAGPVDVASGAAETLRLGVPTRRRGWVALE
                     RFRVETRYPAGLFRAWTWVHMETRCLVYPRPAGAGRPLPDSGSAGISGRPRPGDDDFA
                     GLRAAAAGDPPQRIAWKAYARSDVLLLKEFSSGTGAPCVLDWDMLPELDTEQKLAQLA
                     RWCLDADLAGRSLALRLPNCEVPLGLGPKHLATCLEALALFDDGGAPR"
     gene            complement(2728..>3001)
                     /locus_tag="VFL84_05170"
     CDS             complement(2728..>3001)
                     /locus_tag="VFL84_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7608046.1"
                     /translation="EALLDYVQALLKYTRESGEFVAGLSPRGGIALLRAAQAWALVKG
                     HSGVHPEDLQAVLGSVVAHRLQLTHGSSIERGADLGSAVLAAVPVP"
BASE COUNT          509 a         1061 c         1039 g          392 t
ORIGIN      
        1 ccgatcagca gcgtcagcgt cgcgacgact gcgagaccga gcagcacgcc ggaacgttgc
       61 gcgcggcgca ggttcgtgaa gcccagcgac tcgagcagcg agcgctgcag ctccggaccg
      121 tagccgacga cccacgactg ccagcgcgtg ttgacggcat cccagagcag cgcgatgggg
      181 cggctccagc gcgcgcgcag cgcggccgcc gcggccgtga cgccgccgct cccgaacccg
      241 tcggagccca aggccacgcg ctcgggcgcg accgcggcga cgccgtcgac gcgaacccag
      301 ccttcgtcct cgagccagac ctcggtccac gcgtgcgcgt cggtttgccg gacgatgtag
      361 tagccgccga ctgcgttgag ctcgccgcct tgatatccca tgacgacgcg agcgggcagg
      421 cccgcggctc gcagcaagaa cgtcagcgcc gaagcgtagt gctcgcagaa tccttcgcgc
      481 gtctcgaaca ggaactcgtc gacgggctgc gcgccgagcg ggggcggcgt caacgtgtac
      541 gcaaaaggct gcgacgcgag gtagcccatc gctcgatcga cgatcgtcgc gccgctcggc
      601 gagtcggcca gccagctcgc ggcgagcgcc cgcgcgcgag gattgctgcg ctcgggcagc
      661 gcgcggaaca tcgcgcgctc gttctcgttc aggggctcgc gcgcgcggta atcgacgtaa
      721 gacgtgacgc ggtagtcgag acgccgcgac cgcggtgcgc cgaagggagt gccgagctga
      781 tagtcgctgc ccatgcgcag cgccgaggcg ccgctccact cgcgcggcat gtcgagcgca
      841 aacgcccagt tgcggccgtt cggctcgagc gtcacgcggt agctcgtcgg ctcgccgcga
      901 tactcgatcg tggcggcgac gcgctcaccg cggcgcatgc tgccttggga catcgaccac
      961 gttctgccgt tgaactccgt cagtgaaggg ccgcgccagt agaggtcgcg cgcgcgcggc
     1021 ggccggccgt cgaactggac gcggaaagcg acttcatcgg aaagagcgag attcgtgatg
     1081 tcgccggggc tcatcgtgtc gttcaagccc gtcgcgccgc tgctcgtgct gcccggaatc
     1141 gcccatatcg gccccggcag gcgcggaaac aggacgaaca acacgaccat caccggcatc
     1201 gcgtgcagca acaggcgtcc gctgaagagt cccgtcgcgc ggtacggcag cagcacgccg
     1261 cggcggccga tctggatcag ggctacggtc gcgagccaca cgagcagcac ggtgtaaacc
     1321 gccgccaacg gtccgcggtc ggcgagcaag cgcgcgaaca tcaggaagta gcagatcatg
     1381 atcagcacga gctgatcgcg ctggcttcgc gactcgagca ccttcagccc gatcatgacg
     1441 acgagcaacg cgctgccggc ttcgacgccg ttcaacgtcc gatactggaa cagcacggcg
     1501 agaaagacga tcaacgaaag cggcagccgc acgatgcgcg tggccggcgg ccagccgaag
     1561 aaagcgacgc cgagccgcca cgccacgctg gcgagcaaga acgcgggaat ccatggcgac
     1621 agcgtcggcc agtgcggcaa gctcgcgccg acgacgatcg cgaccgtcca gagcagccgc
     1681 ttcgtctcga gcgacagctc cgtcgcctcc gctgcgagtg cgaacgtcat cgcggcgccc
     1741 cgccgtcgtc gaacaacgcc agcgcctcga ggcacgtcgc gagatgcttc ggcccgagcc
     1801 cgagcggcac ctcgcaattc ggcaagcgca atgcgaggct gcggcccgcg aggtccgcgt
     1861 cgagacacca ccgcgcaagc tgcgcgagct tctgctccgt atcgagctca ggcagcatgt
     1921 cccagtcgag cacgcaggga gcgcccgttc cgctcgagaa ttccttcagc agcagcacgt
     1981 cgctgcgcgc gtacgccttc caggcgatgc gctgcggcgg gtcgccggcc gcggcggcgc
     2041 gcaggcccgc gaagtcatcg tcgccgggcc gcggcctccc gctgattcct gcgctgccgc
     2101 tgtccggcag cggccgcccg gccccggccg gccgtggata cacgaggcag cgcgtttcca
     2161 tgtgcaccca cgtccacgca cgaaagaggc cggcggggta gcgcgtctcg acgcggaagc
     2221 gctcgagggc cacccagccg cggcgccggg tcggcacgcc gagccgcaag gtctcggcgg
     2281 cgccgctcgc gacgtcgacg ggtcccgcgg cgtgctcctt gtgcttgagc tcgatctcga
     2341 gccgcgccgc cggcgcatcg ttgccgacgg cgatcttgaa ctcggctcgc tcgcctgcga
     2401 acacgggtgc cgcgccgagg aacttgatcg aggcgccgag caggttgttg tggcagtgat
     2461 gcatgacgac gagccccaag cccgtcagca ggaacgtcaa cgcgaagccg agactcgctc
     2521 cgtagttcag agagccgagc agcatcgcga ataccatcga cgcgaacacg acgccgaagc
     2581 gcgtcggcag gatgtagatg cggcggcgtc tcagcgcaat cgtgtgcgcg tcggcgcctt
     2641 ggcggcggcg cgcccagacg gcggcccagc gttcgagcga gtgagcgacg tcgagccgca
     2701 tctcgagcta acgccggctg gttgcgctca cggcacgggc acggcggcga ggaccgctga
     2761 tcctaagtcc gccccgcgct cgatcgagct gccatgcgtc agctgcagcc gatgcgcgac
     2821 gacgctgccg agcacggctt gcaggtcttc gggatggacg ccgctgtggc ctttgacgag
     2881 cgcccaggct tgcgcggcac gcaacagcgc gatgccgccg cgcggcgaca agccggcgac
     2941 gaactcgccc gattcgcgtg tgtacttcag caacgcttgg acgtagtcga gcaacgcctc
     3001 c
//
LOCUS       DASSNN010000285         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1185299, whole genome shotgun sequence.
ACCESSION   DASSNN010000285 DASSNN010000000
VERSION     DASSNN010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185299"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFL84_05175"
     CDS             <1..911
                     /locus_tag="VFL84_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7608047.1"
                     /translation="QELVVNDKVQVLAGFGLTPLALATVPIATQAKIPELVMAAATSS
                     ITEQSPYVIRAGFTLPQITVPLAEWAAKNGIKKVVTLVTDYGPGIDAENAFKSRFTAS
                     GGEVTAELRVPLRNPDFAPFLQRAHDAKPDAVFVFVPSGVGSIFMKQFIERGMDKSGI
                     RLIGTGDVTDDDILNGMGDVALGVITSFHYSAAHPSAANKAYVEGYKKLNGTRPNFMS
                     VGGYDGMRIIYESLKKTNGDASGDALLAAMKGLSFESPRGPVTIDPATRDPIQNVYMR
                     KVERVDGELWNVEFATIPSVKDPLHK"
     gene            959..1804
                     /locus_tag="VFL84_05180"
                     /pseudo
     CDS             959..1804
                     /locus_tag="VFL84_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503944.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
     gene            1946..>2508
                     /locus_tag="VFL84_05185"
     CDS             1946..>2508
                     /locus_tag="VFL84_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7608048.1"
                     /translation="MNEIAILALFAVSLDLILGYGGIVSLGHAAFFGTGCYAAALFAK
                     HFMHEPLAGLVVGAAVAAVVGAATSPLVLRGTDLTRLMVTLGVASILYEIANRFSEVT
                     GGADGLQGVVMGPLLGQFAFGLDGKVAYLYSLTMLVLLFLLARRVAHSPFGHSLQAIR
                     DNRLRAGMMGIDVNRRLAVVYTLAAAMA"
BASE COUNT          351 a          828 c          849 g          480 t
ORIGIN      
        1 cgcaggagct cgtcgtcaac gacaaggtgc aggtgcttgc cgggttcggc ttgacgccgc
       61 tcgcgctcgc gacggtgccc atcgccacgc aggccaagat tcccgagctc gtgatggccg
      121 cggcgacgtc gtcgataacc gagcagtcgc cgtacgtcat tcgggccggc ttcacgttgc
      181 cgcagatcac cgtgccgctc gccgaatggg cggcgaagaa cggcatcaag aaggtggtca
      241 cgctggtcac cgactacggc ccgggtatcg acgccgagaa cgcgttcaag tcgcgcttca
      301 ccgcctcggg aggcgaggtc acggccgagc tgcgcgtgcc gttgcgcaat cccgattttg
      361 ccccgttcct gcagcgcgcg cacgacgcca agcccgatgc cgttttcgtg ttcgtgcctt
      421 ccggcgtcgg ctcgatcttc atgaagcagt tcatcgaacg gggaatggac aagagcggca
      481 tcaggctgat cggcactggc gacgtcaccg acgacgacat cctgaacggc atgggcgacg
      541 tcgcgctcgg cgtgatcacg tcgttccact actcggccgc gcatccgtcg gcggcgaaca
      601 aggcttacgt cgagggttac aagaagctca acggcacgcg accgaacttc atgtcggtgg
      661 gcggctacga cggtatgcgg atcatctacg aatctctcaa gaagacgaac ggcgacgcga
      721 gcggcgacgc gctgctggcc gcgatgaagg gcctttcgtt cgagagcccg cgcggcccgg
      781 tcacgatcga cccggcaacg cgcgacccca tccagaacgt gtacatgcgc aaggtcgagc
      841 gcgtcgatgg ggagctgtgg aacgtcgagt tcgcgacgat tccgtcggtg aaggacccgc
      901 tgcacaagtg actgcggtct gcccgatcgt aacgatctga cgatcgaggt ctaaggcgat
      961 gctgacgatt cttctcgacg gcatcgccta cggcatgctg ctgttcctga tcgcgagcgg
     1021 tctcgccgtg acgctcgggc tcatgaactt cgtgaacctc gcgcacggcg cgttcgcgat
     1081 ggccggtggc tacgttgccg ctgtgctgtc caaccgcgtc ggtgtgccgt tcctgctcac
     1141 gctgcccatc gcgttcgcgg ccggcgcggc gctcggcgcg gcgctcgagc ccgcgctcta
     1201 tcgccgcatg taccgccggc cgcacctcga ccaggtgctg ttcaccatcg ggctcgtgtt
     1261 catggcggtg gccgccgtcg atttcgcatt cggtgcgacg cagcagtacg tcaagcttcc
     1321 ggtgtatctg tccggacgca tcgaggtggc cggcgtcggc atcggcgtct accggctgtt
     1381 catcatcgcg gtctgcggcc tgcttgcggc gctgctgcaa tggacgctcg tctcgacgcg
     1441 gctcggcagc cgcctgcgcg cggccgtcga cgacacgcgg gtcgcgagcg gtcttgggat
     1501 caacgtcaac gcgattttca tgtcgacttt tgccttcggc tcaggactcg cggggctcgg
     1561 cggcgcgctc ggcgccgagt tgctcgggct cgatccgacg tttcccctca aatacatcat
     1621 ctatttcctg gtggtcgtcg ccgtgggcgg cacgacgacg atcaccgggc ccctcgtcgc
     1681 gtcgcttctg ctcgggatcg ccgacgtcgc cggcaagtac tacgtgccga agttcggtgc
     1741 gttcatcatc tacagcgtga tgatcgcggt gctcgtcgtg cgcccgcagg gcctgttcgc
     1801 gcgcgcgacg ggcgcagctt cggccgcggc gcccgtggcg cacgcgctgc cggccgtgcg
     1861 cgcgtcgcgc ctgcggccct gggagatcgt gtggccgatc gtcgcgctgg ccgcgctggt
     1921 gatgtttccg cggcacgcgg cgctgatgaa cgagatcgcg attctcgcgc tgtttgccgt
     1981 gtcgctcgat ttaatcctgg gctacggcgg gatcgtctcg ctcggccacg cggcgttctt
     2041 cggtaccggc tgctatgcgg cggcgctgtt cgcgaagcac ttcatgcacg agccgctcgc
     2101 cggcctcgtc gtcggcgcgg cggtggccgc cgtcgtgggc gcggcgacaa gtccgctcgt
     2161 cctgcgcggc accgatctca cgcggctgat ggtgacgctc ggcgtggcgt cgattctcta
     2221 cgagatcgcc aatcgcttca gcgaggtgac gggcggcgcc gatggcctgc agggtgtggt
     2281 gatgggtccg ctgctcgggc aattcgcgtt cggcctcgac ggaaaggtgg cctacctgta
     2341 cagcctgacg atgctcgtgc tgctcttcct gctcgcacgg cgcgttgcgc attcgccgtt
     2401 cggtcattcg ctgcaggcca ttcgcgacaa ccggctgcgg gcgggcatga tgggcatcga
     2461 cgtgaaccgc cggctcgccg tcgtgtacac gctcgctgcg gcgatggc
//
LOCUS       DASSNN010000286         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1296088, whole genome shotgun sequence.
ACCESSION   DASSNN010000286 DASSNN010000000
VERSION     DASSNN010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296088"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1473)
                     /locus_tag="VFL84_05190"
     CDS             complement(91..1473)
                     /locus_tag="VFL84_05190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7608049.1"
                     /translation="MSLFWKIFVSFMIAMTITSVGAIYVTVQLVNQPLTLSDFEGRDR
                     IIREMSAALARGGEFELKRWLFDNLRPAPGTVVLVTNERGDELLGRAMPRELRALLRF
                     RPPNRRGDTPPNLRPMQLTPSLIGPDGAEYRLTFARAPLEMYGILIWPGTQVAVLSFA
                     ILTAAVLSALLARYVSSPIARLQKASRGLAAGALDTRVGAPFNRRRDEVGTLARDFDA
                     MAERIQELVTAKETLLRDVSHEFRSPLARIRMALALAERRAGAESQPDLARIEREAER
                     LDALVGQVMTLTRLRTTDAPRRDVVRLDQLVGEVVDDARFEYPDATLEYTPNAEVSLR
                     GDGDGLKSAIENVVRNAMIYGDRTKPIEVRIDSTAAAATVRVLDRGPGVPEAELERIF
                     EPFYRTDKSRDHRQDGQGIGLAITARVTELHGGKVRARNRPDGGLEIAIELPREGPDL
                     SSAENRSVPR"
     gene            complement(1476..2174)
                     /locus_tag="VFL84_05195"
     CDS             complement(1476..2174)
                     /locus_tag="VFL84_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7608050.1"
                     /translation="MPDNRLLIIDDDVRLAEMLTEYLAPEGIELTSVASGIQGLRSAQ
                     RENYDLIILDVMLPGLSGFDVLKQLREAGVKTPVLMLTARGDDVDRIVGLDLGADDYL
                     PKPFNPRELLARVKAILRRTAEPEGDEPVEISVGDLRVNLRRREAWLGESPLKLTNAE
                     FVILVTLMRSPGDVVSREALTRTALGRQLLPDDRSLDTHISNLRRKLGSDADADVLIR
                     SIRGSGYVLVPPEK"
     gene            complement(2262..>2609)
                     /locus_tag="VFL84_05200"
     CDS             complement(2262..>2609)
                     /locus_tag="VFL84_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7608051.1"
                     /translation="LEGILEAAAAAGAGNAGYVLLRLPHEVAPLFVEWLREHYPDRAE
                     HVLSLVKQLRGGELYDSRWGTRQRGLGPFAALLKTRFEKARRRHGLEREVALRCDKFV
                     PPRPPKRQIELGF"
BASE COUNT          409 a          880 c          909 g          411 t
ORIGIN      
        1 gacccgccgc caaaaagggg acgcgccaaa aaggggacgg atttattttc tcggaaaata
       61 gatctgtccc ccttttcggg gtgcagcctt ctagcggggg acggatctat tttcggcaga
      121 cgatagatcc ggcccttctc ggggaagctc gatcgcgatc tcgagcccgc cgtcgggccg
      181 attccgcgcc cggaccttgc cgccgtgcag ctcggtcacg cgcgccgtga tcgcgaggcc
      241 gattccctgg ccgtcttggc gatggtcgcg gctcttgtcg gtgcggtaga acggctcgaa
      301 gatgcgctcg agctcggcct ccggtacgcc gggtccgcgg tcgagcacgc gcaccgtcgc
      361 cgcggcggcg gtgctgtcga tccggacctc gatcggcttc gtgcggtcgc cgtagatcat
      421 cgcgttgcgc acgacgttct cgatcgcgct cttcaatccg tcgccgtcgc cgcgcagcga
      481 gacctccgcg ttcggcgtgt actcgagcgt cgcgtcggga tactcgaacc gcgcgtcgtc
      541 gacgacctcg ccgacgagct ggtcgagccg cacgacgtcg cggcgcggcg cgtcggtcgt
      601 gcgcaagcga gtcaacgtca tgacctgccc gacgagcgcg tcgaggcgct cggcctcgcg
      661 ctcgatgcgc gcgaggtcgg gctgcgactc ggcgcccgcg cggcgctcgg cgagcgcgag
      721 cgccatgcgg atccgtgcga gcggcgagcg gaactcgtgc gagacgtcgc gcagcagcgt
      781 ctccttcgcc gtgacgagct cttggatgcg ctcggccatc gcgtcgaagt cgcgcgcgag
      841 cgttccgacc tcgtcgcggc gccgattgaa cggcgcgccg acgcgagtat cgagggcgcc
      901 ggccgcgaga ccgcggctcg ccttctgcag ccgcgcgatc ggcgacgaga cgtagcgcgc
      961 gagcagcgcc gagagaacgg ccgccgtcaa tatcgcgaac gacagcaccg cgacttgcgt
     1021 gcccggccaa atcaagatgc cgtacatctc gagcggtgcg cgcgcgaacg tgaggcgata
     1081 ctccgcgccg tccggcccga tcagcgacgg cgtcagctgc atcggccgca agttcggcgg
     1141 cgtgtcgccg cggcggttcg gcggccggaa gcgcagcagc gcgcgcagct cgcgcggcat
     1201 cgcgcggccg agcagctcgt cgccgcgctc gttcgtcacg agcaccacgg tgccgggcgc
     1261 cggccgcaga ttgtcgaata gccaacgctt gagctcgaac tcgccgccgc gcgcgagcgc
     1321 ggcggacatc tcgcggatga tgcggtcgcg gccctcgaag tccgacagcg tcagcggttg
     1381 attgacgagc tgaacggtca cgtagatcgc gccgaccgac gtgatcgtca tggcgatcat
     1441 gaacgagacg aagattttcc agaacaggct cacgcttact tctcgggtgg cacgagcacg
     1501 tagccgctgc cgcgaatgct gcgaatcagc acgtcggcat cggcatcgct gccgagcttg
     1561 cggcgcaggt tgctgatgtg cgtgtcgagg ctgcggtcgt cgggcaagag ctgccggccg
     1621 agcgccgtgc gcgtcagcgc ttcgcgcgac acgacgtctc cgggcgagcg catcagcgtc
     1681 acgaggatca cgaactcggc gttcgtgagc ttgagcgggc tctcgccgag ccacgcttcg
     1741 cggcggcgca ggttgacgcg cagatcgccg acgctgatct cgaccggctc gtcgccttcg
     1801 ggctccgccg tgcggcgcag gatcgccttg actcgcgcga gcagctcgcg cggattgaaa
     1861 ggcttcggca agtagtcgtc ggcgccgaga tcgagcccga cgatgcgatc gacgtcgtcg
     1921 ccgcgcgccg tcaacatcaa cacgggcgtc ttcactccgg cttcgcgaag ctgcttgagg
     1981 acgtcgaaac cggacagccc cggcagcatc acgtcgagga tgatcaggtc gtagttttcg
     2041 cgctgcgcgc tgcgcagccc ctgaataccg gaggccacgc tcgtgagctc gatgccctcg
     2101 ggcgcgaggt attcggtgag catctcggcc aggcgcacgt cgtcgtcgat gatgagaagg
     2161 cgattgtcgg gcacggctag gaccccgcga attgctgcaa agtgccacaa attccggcgc
     2221 cgaccctagt cggcggccgg gccgaacgag aaatattgag actaaaaccc aagctcgatt
     2281 tgccgcttag gcgggcgcgg cggcacgaat ttatcgcatc tgagcgcgac ttcgcgctcg
     2341 agcccgtgac gccggcgggc cttctcgaac cgcgtcttca ggagcgccgc gaacggcccg
     2401 aggccgcgct gccgggtgcc ccagcgcgag tcgtagagct cgccgccgcg cagctgcttg
     2461 acgaggctca gcacgtgctc ggcgcggtcc ggatagtgct cgcgcagcca ttcgacgaac
     2521 aacggcgcga cctcgtgcgg gaggcgaagc agcacgtagc cggcattgcc ggcgccggcg
     2581 gccgcagccg cttcgaggat gccttcgag
//
LOCUS       DASSNN010000287         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_55654, whole genome shotgun sequence.
ACCESSION   DASSNN010000287 DASSNN010000000
VERSION     DASSNN010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55654"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VFL84_05205"
     CDS             <1..443
                     /locus_tag="VFL84_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7608052.1"
                     /translation="FLTCYRDNLFVRSRLYPGVVETLDALRARGLKLCCVTNKRYAFS
                     HDLLVAAGIRDYFDPLLGGDSLPEKKPSPLQLKVAAEKTGVAPAAAVLVGDSHQDLRA
                     ARAAGCGFVLASYGYGKIDETELGASPRIRSFADLPAMLGIGRV"
     gene            complement(470..889)
                     /locus_tag="VFL84_05210"
     CDS             complement(470..889)
                     /locus_tag="VFL84_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608053.1"
                     /translation="MSEWIIDTGPLVAYLDAADDEHARVAACLDKFSGRLVTTGAVVT
                     EAMYFVSTTTSGARRLADLVADSGMHVFDLAQPAELRAAAELMERYSDTPMDYADATL
                     VLLAEALQVYDVLTLDRRGFSTYRPARGKAFRLTLDP"
     gene            1239..1988
                     /locus_tag="VFL84_05215"
     CDS             1239..1988
                     /locus_tag="VFL84_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608054.1"
                     /translation="MNTRSIVVLVVVGTAAIAAVGLWRASLSVGDTERVASDFQTPAS
                     ASASASAASTNGDNAPTEALVIDPAWRTPSSPSPRYAEPEEIERQLEQFIAEQPGLEI
                     VSLSSIECGATTCEIALTGTEVNPRYVDAYSELNRRLSQGPWTDFIIRSGGLSTREIA
                     PGAREYVIGFDYRPYVDLSADPLIAARQYAACAAFWRQQTEFPAPDDVVRGYLERVDH
                     YVALAASVLGEDEAARIAADNRGAPVIRGCW"
BASE COUNT          330 a          693 c          681 g          356 t
ORIGIN      
        1 cttttctgac gtgttaccgc gacaacctgt tcgttcgcag ccgcctctat cccggcgtcg
       61 tcgagacgtt ggacgcgctg cgcgcgcgcg gcctgaagct gtgctgcgtc acgaacaagc
      121 gctacgcgtt ctcgcacgac ctgctcgtcg cggccgggat ccgcgactac ttcgatccgc
      181 tgctcggcgg cgacagcctg cccgagaaaa agccaagccc gttgcagctc aaggtcgcgg
      241 cggagaagac cggcgtcgcg ccggccgccg cggtgctcgt cggcgactcg catcaggact
      301 tacgcgccgc gcgcgcggcg ggttgcggct tcgtgctcgc gagctacggc tacggcaaga
      361 tcgacgagac cgagctcggc gcgagcccgc gcatccgcag cttcgccgac ctgcccgcca
      421 tgctcggcat cggccgcgtc taggtggcgg cctcagcgcc cgagggaagt cacggatcga
      481 gcgtcagcct aaacgccttg ccgcgggcgg gtcgataggt cgaaaagcca cggcgatcga
      541 gcgtaagcac gtcgtagacc tgcaatgcct ccgccagcaa gacgagcgtc gcatccgcgt
      601 agtccatcgg agtgtctgaa tagcgctcca tgagctcggc cgcagcccga agctcggccg
      661 gctgcgccaa atcgaagacg tgcatgccgc tgtccgcgac gagatccgcg aggcgccggg
      721 ctccggaggt ggtcgtggac acgaagtaca tggcttccgt tacgaccgcg ccagtcgtga
      781 ctagccgtcc cgagaacttg tccagacatg cggcgacccg agcgtgctcg tcgtcggcgg
      841 cgtcgaggta ggcgacgagc ggtccggtgt cgataatcca ttcgctcacg gacgccaatt
      901 tcgctcgcga atttggcgct gccacgcgcc acgcgcttcg gcgatacgga gactgccccg
      961 tgtcttcccg agccgctcgg ccaacggccg ttcgacgagg gcgctttcga gaatctcgcg
     1021 gacgacctcg gagaccgtct ttctttcgat cttggccttc ctgatcaagg cgtcgcgcag
     1081 cgacttgtcc gcgcgaatcg tgattgtcgt actcatgtcg tacagcgtac gacacgcgcc
     1141 ctttcgcgag caatcctaat atcgacggca atcgcctaga ttgcgcaaac gaacgggcgg
     1201 ctctaccgtt gcgacggacc gtagtgtccg gcaatggcgt gaacactcgt tcgatcgtcg
     1261 tgctcgtcgt cgtgggaacg gccgcgatcg ccgcggtcgg cctctggcgc gcgtccttgt
     1321 cggttggcga cactgaacgc gtagcgtccg attttcagac gcctgcatcg gcgagcgcat
     1381 cggcaagcgc ggcctcgacg aacggcgata atgcgccgac cgaggcgctt gtgatcgacc
     1441 cggcgtggcg cacgccgtcg tcgccgagcc cgcgctatgc cgagcccgag gagatcgagc
     1501 gacagctcga acagttcatc gccgagcaac ctgggctcga gatcgtgagc ttgtcgtcga
     1561 tcgagtgcgg cgcgacgacc tgcgagatcg cgttgacggg aaccgaggtc aatccgcgct
     1621 acgtcgacgc gtattcggag ttgaacagaa ggttgtcgca agggccgtgg acggacttca
     1681 tcattcggtc cggcgggctc agcacacgcg agattgcacc cggcgcccgc gagtacgtga
     1741 tcggcttcga ctatcggccc tacgtcgatc tgagcgccga cccgctgatc gccgcgcgtc
     1801 agtacgccgc gtgtgcggcc ttttggcggc agcagaccga gttcccggca cctgacgacg
     1861 tcgtgcgcgg ttacctcgag cgagtcgacc actacgttgc gctcgcagcg agcgtgctcg
     1921 gcgaagacga agccgcgcgc atcgccgctg acaatcgtgg cgcgccggtg attcgcggct
     1981 gttggtgacg gcgggctcgc cgcgccgctc agcgcttctg cgctgccgcc gccgcgcgct
     2041 cttcggcgcg cgcggcgctc
//
LOCUS       DASSNN010000288         4737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1296449, whole genome shotgun sequence.
ACCESSION   DASSNN010000288 DASSNN010000000
VERSION     DASSNN010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296449"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..836
                     /locus_tag="VFL84_05220"
     CDS             69..836
                     /locus_tag="VFL84_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608055.1"
                     /translation="MKTSSIVVLVVVGVAAVAAVGLWGRRPLPTDDSENGAPDWAASV
                     APAPAIASPRASSTNADEVAAEPPAIDPARRTLSSPNPRYAEPDEIERLLAQFIAQQP
                     GLGIVSLSSIECGATSCEIALTGAEVNPRYVGAYSDLYRKLSSAGGNDFLIRSAGLGT
                     REIAPGAREYVISFDYRPYVDLSADPLIAARQYAACAAFWRQQTEFPAPDDVVSGYLD
                     TAEHYVALAASVLGEEEAARVAADTRGAPVLRNCLAR"
     gene            complement(837..1832)
                     /locus_tag="VFL84_05225"
     CDS             complement(837..1832)
                     /locus_tag="VFL84_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608056.1"
                     /translation="MSKAYWATAGAAAAVACAVASAQEAPRTAWGDPDLRGTYTSDNS
                     IGVPFERPAQFGERAELTDEEYAAKVDANEAQIAKDLNPEPESEFSAEDPSAINASRH
                     WLERPETPSRATSLVVDPPNGRLPELTPAGQQRAAERRARRPRGQLPASYTDFTNYDR
                     CLTRGVVGSILPVIYGNGTQIVQAPGVVVIRNEMIHEARVIPLDGSAHLGDGVHMWMG
                     DSRGRFDGDTLVIETTNFTDRTGLGGNGNGAVHSENMKLVEKLRRVDADTLRYEFTVD
                     DPETYTKPWTVAFDLDGKPGYEIYEYACHEGNLGLANMLSAARAEERAAAQATKR"
     gene            1997..4051
                     /locus_tag="VFL84_05230"
     CDS             1997..4051
                     /locus_tag="VFL84_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7608057.1"
                     /translation="MLSIVARDRARAMPIQKLFAAAMLLTLAACSPSEDSVGTETAAA
                     PAGNFDFEGWDQYLGGADSSQFSSLKQIDKSNVGRLEVAWTFPTGDNYSFNPLIVGNT
                     MYVLASEPGGARPARRQIVALDAATGRQLWAHSNEGAVGTRGMNYWRSPDGSEERLLY
                     LNAGYLTAIDAKTGETITSFGVDGRVDLRIGLDGDITTIRALQTNNPGRIFEDTIIMS
                     LPAGGGGYASAPADIHAYDVRTGAQRWIFHTVPRPGEFGADTWPEAGLGNYGGVHNWS
                     ESTIDKELGIVYIPTGTARYDFYGGNRHGANLFGNSLLALDARSGKRLWHFQTIHHDL
                     WDYDLPTAPKLLNIVHDGVTVPAIAQPSKQGFVYVFNRITGAPVWPIEERPVPQSDTP
                     GEQSWPTQPFPTAPPPFARQRFTEADINPYISEEDQAKVREFLKSARNEGLFTPPSLQ
                     GTVMLPGHNGGANWGSSAVDPTKGRLFVVSKELPTTANLRAPQPPAPGVGPGPGGGPP
                     RGGQGGGGNRPPPLPPPNAGPDFIPYTAPADFMIQPSSGLSAIGPPWSQITAYDLNKG
                     TIMWQIPDGDVASLAAKGITGTGSHAPRGGVVATGGGLLFIGTSSDRKFRAYDADTGK
                     VLWSYDLPAATEGVPAVYSVAGKQYVAIAVGGNGLFSQGMNLPEPGPGQYMVFALGGG
                     NQ"
     gene            4072..4575
                     /locus_tag="VFL84_05235"
     CDS             4072..4575
                     /locus_tag="VFL84_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7608058.1"
                     /translation="MRTLTTLVVTAVALGANAAFADDAALVREAQDRAAIEALMWRYV
                     RALDSMDADAYAAVFTEDGAFKSGSTQHKGRAEMKEMIADLKKTRDERAAAGTTSPPM
                     YHVIANHQITFTGPNEARYDSYWMTVFGAAGPNTPVRVAAAGRGVDQLVRVNGQWLIK
                     NRDVAPQ"
BASE COUNT          761 a         1628 c         1594 g          754 t
ORIGIN      
        1 tagcgtctag cttgcggaat cgaacacgcg gctctaccgt tgcgatagac ggtagtgtcg
       61 ctcatcgcgt gaagactagc tcgatcgtcg tgcttgtcgt cgttggcgtg gccgcggtcg
      121 cggcggtcgg cctctggggg cgccggccct tgcccactga cgacagcgag aatggcgcgc
      181 ccgactgggc tgcctccgtt gcgccggcgc ctgcgatcgc atcaccccgc gcgagctcga
      241 cgaacgccga cgaggttgcg gcggagccgc ccgcaatcga tccggcgcgg cgaacgctgt
      301 cgtcgccgaa cccccgctac gccgagcccg acgagatcga gcggctactc gcgcaattca
      361 tcgcccagca accaggcctc gggatcgtga gcttgtcgtc gatcgagtgc ggcgcgacga
      421 gttgcgagat cgcactcacg ggcgccgaag tcaatccgcg ctatgtcggc gcgtattcgg
      481 atctgtaccg caagttgtcc agcgcgggcg ggaatgactt cttgatccgg tccgccggcc
      541 tcggcacacg cgagatcgcg ccgggcgctc gcgagtacgt gatcagtttc gattatcggc
      601 cgtacgtcga tctgagcgcc gacccgctga tcgccgcgcg tcagtacgcc gcatgtgcgg
      661 cgttttggcg gcagcagacc gagttcccgg cgcccgacga cgtcgtgagc ggttatctcg
      721 acacggccga acattacgtt gcgctggcag cgagcgtgct cggtgaagaa gaagccgcac
      781 gagtcgctgc ggatacccgt ggcgcgccgg tgcttcgcaa ttgtctcgcg cgctaatcag
      841 cgcttggttg cttgcgccgc ggcgcgctct tctgcgcgcg ccgcggagag catgttcgcg
      901 agccccaagt tgccttcgtg gcaggcgtat tcgtagatct cgtagcctgg cttgccgtcg
      961 aggtcgaatg cgacggtcca cggcttcgtg tacgtctcgg gatcgtcgac cgtgaactcg
     1021 tagcgcagcg tatcggcgtc gacgcgccgc agcttctcga cgagcttcat gttctcgctg
     1081 tgcacggcgc cgttgccgtt gccgccgaga cccgtgcgat ccgtgaagtt cgtcgtctcg
     1141 atgacgagcg tgtcgccgtc gaaccggccg cgcgagtcgc ccatccacat gtgcacgccg
     1201 tcgcctaagt gcgcgctgcc gtcgagcgga atcacgcgcg cctcgtggat catctcgttg
     1261 cggatcacga cgacgccggg cgcctggacg atttgcgtgc cgttgccgta gataacgggg
     1321 aggatcgagc cgacgacgcc gcgcgtgagg cagcgatcgt aattagtgaa gtcggtgtac
     1381 gacgccggca gctgcccacg cgggcgccgc gcgcggcgct ccgcggcacg ctgctggccc
     1441 gcgggcgtca gctccggcaa tcgcccgttc ggcgggtcga cgacgagcga cgtcgcgcgt
     1501 gaaggcgtct cggggcgctc gagccagtga cgggatgcgt tgatggccga cgggtcctcg
     1561 gccgagaact cgctctcggg ctccggattc aggtccttcg cgatctgcgc ctcgttggcg
     1621 tcgaccttgg cggcgtactc ctcgtccgtg agctcggccc gctcgccgaa ttgcgccggc
     1681 cgctcgaacg gcacgccgat cgagttgtcg ctcgtatacg tgccgcgtag atcggggtcc
     1741 ccccacgccg tacgcggagc ctcttgcgcg ctcgcgacgg cgcaggcgac cgccgccgcg
     1801 gcccccgccg tcgcccagta ggccttgctc atcgcctgct ccgtagctcg acctccgaac
     1861 gcaaaagcat aagccccgcc tgcttggaat gcgcacgttc cctgggtttc ttcagactcg
     1921 ccggactgcg tatcgcggcg gcctgctggt agtatccccg tcggattcca cgggttcgtg
     1981 cagggggagg cagtccatgc tctcgatcgt cgccagggat cgcgctcgcg cgatgccgat
     2041 tcaaaaacta ttcgcggctg cgatgctact cacgctagcc gcttgctcgc cgtcggagga
     2101 cagcgtcggc accgaaacgg ccgccgctcc ggccggcaac ttcgacttcg aaggctggga
     2161 tcagtatctc ggcggcgccg actcgtcgca gttctcgtcg ctgaagcaga tcgacaagag
     2221 caacgtcggc cggctcgagg tcgcgtggac ttttccgacc ggcgacaact actcgttcaa
     2281 tcctttgatc gtgggcaaca cgatgtacgt gctggcgagc gaacccggcg gcgcccgccc
     2341 cgcccgccga cagatcgtag cgctcgacgc tgcgaccggc cgccagctct gggcacactc
     2401 caacgaaggc gccgtcggca cgcgcggcat gaactattgg cgcagccccg acggcagcga
     2461 agagcgcctt ctgtacttga acgccggcta cctgaccgcg atcgacgcga agaccggcga
     2521 gacgatcacg agcttcggcg tcgacggccg cgtcgacttg cgcatcgggc tcgacggcga
     2581 catcacgacg attcgcgcgc tgcagacgaa caatcccggc cgaatcttcg aggacacgat
     2641 catcatgtcg ctgccggccg gcggcggcgg ctacgcatcg gcgccggccg acattcacgc
     2701 ctacgacgtg cgcaccggcg cacagcgctg gatcttccac acggtgccgc gccccggcga
     2761 gttcggcgcc gacacgtggc ccgaagccgg gctcggcaac tacggcggcg tgcacaactg
     2821 gagcgagtcg accatcgaca aggagctcgg catcgtctac atcccgacgg gcacggcgcg
     2881 ctacgacttc tacggcggca accgccacgg cgcgaatctg ttcggcaaca gcctgctcgc
     2941 gctcgatgcg cgcagcggca agcgcctctg gcacttccag acgatccatc acgatctctg
     3001 ggactacgac ctgccgacgg cgccgaagct gctgaacatc gtgcacgacg gcgtcacggt
     3061 tcccgccatc gcgcagccga gcaagcaagg cttcgtctac gtcttcaacc gcatcacggg
     3121 cgcgccggtc tggccgatcg aggagcggcc cgtgccgcag tccgatacgc cgggcgagca
     3181 gagctggccg acgcagccgt tcccgacggc gccgccgccg ttcgcgcgcc agcgcttcac
     3241 ggaagccgac atcaatccgt acatctcgga agaggatcaa gcgaaggtcc gcgaattctt
     3301 gaagtcggcg cgcaacgaag gcttgttcac gccgccgagc ctgcaaggca ccgtcatgct
     3361 gcccgggcac aacggcggcg cgaactgggg cagctcggcc gtcgacccga cgaagggcag
     3421 gctcttcgtc gtctcgaagg agctgccgac gacggcgaac ctgcgcgcgc cgcagccgcc
     3481 cgcgcccggt gtcgggcccg gccccggcgg cggaccgccg cgcggcggcc aaggcggcgg
     3541 cggcaatcgt cctcccccgc tacctccgcc gaacgcgggt ccggatttca ttccgtacac
     3601 ggcgccggcc gacttcatga tccagccgag ctcgggcctg tcggcgatcg gcccgccgtg
     3661 gtcgcagatc acggcgtacg acctgaacaa gggcacgatc atgtggcaga tcccggacgg
     3721 cgacgtcgcg agcctcgctg cgaagggcat cacgggcacg ggcagccacg cgccgcgcgg
     3781 cggcgtcgtc gcgacgggcg gcggcttgct gttcatcggc acgtcgtcgg atcgcaagtt
     3841 ccgcgcctac gacgccgaca cgggcaaggt gctctggagc tacgacttgc cggccgcgac
     3901 cgagggcgtg ccggccgtgt actcggtcgc cggcaagcag tacgtcgcga tcgcggtagg
     3961 cggcaacggt ctgttcagcc aaggcatgaa cctgcccgag cccggcccgg gccagtacat
     4021 ggtgttcgcg ctcggcggcg gcaatcagta acaactgacc ggagaacgca gatgcgtaca
     4081 ttgacgactc tcgtggttac agcggttgcg ctcggcgcga atgccgcgtt cgccgacgac
     4141 gcagcgctgg tgcgcgaagc gcaagatcgc gccgcgatcg aggccttgat gtggcgctac
     4201 gtgcgcgcgc tcgactcgat ggatgccgat gcgtacgcgg ccgtgttcac ggaagacggc
     4261 gcgttcaaga gcggcagcac gcagcacaag ggtcgcgccg agatgaaaga gatgatcgcc
     4321 gacctcaaga agacgcgcga cgagcgcgcc gccgccggca cgacgagccc gccgatgtac
     4381 cacgtgatcg cgaaccacca gatcacgttc acggggccga acgaagcgcg ttacgactcg
     4441 tattggatga cggtgttcgg cgcggcgggt ccgaacacgc cggttcgcgt cgcggccgcc
     4501 ggccgcggcg tcgaccagct cgtgcgcgtc aacggccagt ggctgatcaa gaaccgcgac
     4561 gtcgcgccgc agtgagcggc ggctagctcg accaagcgcc gggtcgccct ggggattgcg
     4621 ccccagggct cccacagatc cggacgtgcg gatttcccgc atccggctct tgagcgtcaa
     4681 ggatttgctg cgcatcggta aacactgtga acgacccgcg cgggcggaag cgggtag
//
LOCUS       DASSNN010000289         6503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1567408, whole genome shotgun sequence.
ACCESSION   DASSNN010000289 DASSNN010000000
VERSION     DASSNN010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567408"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VFL84_05240"
     CDS             complement(<1..370)
                     /locus_tag="VFL84_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7608059.1"
                     /translation="MQETSSAQAEDFASVLRERRSIDLFAPDAVGVDTLFAAIEVARW
                     APNHRLTEPWRFYLLGPATRRAVVDLAVELEVAKKGERAGPVRRARLESVPGMFVLTS
                     ARSDDALLDRENYAACCCAAQ"
     gene            complement(437..1447)
                     /locus_tag="VFL84_05245"
     CDS             complement(437..1447)
                     /locus_tag="VFL84_05245"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET7608060.1"
                     /translation="MSSPATGSAIRTTAISIALITFAACTTHVPLSDDYRGPDTWPAG
                     TPSVYDDLVYDDLESAPLETEEDGEGRTRAHFVVRQLSLPSPTDPAASIDFEYYDVDG
                     DARTPVVMLLPIFNGELMIPRFFARYFAKQGWAAVVVTRGRDPLDALTEPESALVGNL
                     HDYHRVLDWIEHEPELDASRIGVFGVSLGAMDAIMLTALDHRVNSLVVAMAGGDLPYL
                     LANTNYRRVVRSIDEMASELGTSREALGEKLGAAMKLDPLDVAPYIDAERVFMVLTRT
                     DAIIPFKAQEQLRETMGSPESMYLLTGHRTSVFYFPKVRTAAYEFFARRFALQQVALA
                     MN"
     gene            complement(1604..2194)
                     /locus_tag="VFL84_05250"
     CDS             complement(1604..2194)
                     /locus_tag="VFL84_05250"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7608061.1"
                     /translation="MSESQHSHTSDDDFGHFLKGWIRNPLAIGAFAPSGKSLAKLMAR
                     DVGVGSRVVELGAGTGRVTAALLASGVAPGNLYLVERDPHFVQLLERRFPRCHVVAAD
                     ALELDSALDAQKFDFVISGLPLLCFSPEKRYRILQQALRLLKPQGRLHQFTYAGRCPV
                     DRDLRALLRVDSVLLGIAPLNLPPGFVYRLTPRLAA"
     gene            complement(2247..2849)
                     /locus_tag="VFL84_05255"
     CDS             complement(2247..2849)
                     /locus_tag="VFL84_05255"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HET7608062.1"
                     /translation="MRAKLWVVALSVVAASGCSPGTPEVAAPAPPAGGEWQTLFDGSS
                     LAGWTQVGDANWQLEDGAVAADSGTGFLLTAGSYADFDLDLEFWVTPDANSGVFIRCA
                     NPSEIGAATCYEVNIFDQRPDPTYRTGAIVEVAAPKVMLETGDKWNHYEISADGRHLV
                     VRLNGQTTVDTNDDKLAAGPFALQYGAGRVMFRNVRIRAK"
     gene            complement(2963..4315)
                     /locus_tag="VFL84_05260"
     CDS             complement(2963..4315)
                     /locus_tag="VFL84_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7608063.1"
                     /translation="MANVLLVDDEPDFVAAAKEMLQLHGHTVESAERLESARRLLGGA
                     APDVLLLDLMLPDGNGLELLEELRASPVKRVVFITGHPGIKSLIKNLSGPSVSYLTKP
                     IGSTELLRTVEADGSTQNGSATPNGNLHFGCLIGESPAMLAVYEQIDKVARTPSPVLI
                     LGETGTGKELVAESIHRASGRPGQFVAVNCGSLSRELAASELFGHEKGSFTGAVRRHP
                     GFFKRADGGTLFLDELAEMPVELQPHFLRVLETGSVLPVGSEREVPIDARTVAATNRD
                     PKKAIADKALREDLYYRLSVFPITLPPLRARIQDVPLLVDHFLGQAQTASGPETRRFS
                     AEDSARLRGYHWPGNVRELRHVVLRAAIMTDPNEALLRLPERFDSPFNEPNGQQGLAI
                     GRSIRDVERELIMRTLEHLNGDKRAAAETLGISLNTLYNRLNAYEAAGMAAPAAPPMP
                     "
     gene            4613..5347
                     /locus_tag="VFL84_05265"
     CDS             4613..5347
                     /locus_tag="VFL84_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7608064.1"
                     /translation="MSELQDNTFLSWLSHELRSPLNACVMWVDVLALSPQPDKLAKAV
                     DAIKRNLARQARLVNDLNDAAKISSGGIELRTEPFDLVALLRHELDAWQSLAAGKQLE
                     LHPSVELDSAPVVGDSARLAQAVNHLVENAIASTPSNGRVDVRVHGADGNCVVEVADT
                     GAGLSTEDAANLGTPLWRASTTARARAGIGLGLAVAHHVAGKHGGSLTATSAPSGAHF
                     VLTLPLARSGIDSVAKMERTSDLSDL"
     gene            complement(5344..5592)
                     /locus_tag="VFL84_05270"
     CDS             complement(5344..5592)
                     /locus_tag="VFL84_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608065.1"
                     /translation="MLEPTNHRDPSAPREPELPVVDDPVDPGEPKIPEMPPPVPDPPE
                     IVGVGYGSEEGYYRLEGWEPASWQPHPQDGADRGAARG"
     gene            5815..5982
                     /locus_tag="VFL84_05275"
     CDS             5815..5982
                     /locus_tag="VFL84_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608066.1"
                     /translation="MNNEIEPDDDSYEPAGKGAARRRVDAYHEVLRERAAMLELDAPF
                     VETRSWPPRRY"
     gene            6000..6188
                     /locus_tag="VFL84_05280"
     CDS             6000..6188
                     /locus_tag="VFL84_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608067.1"
                     /translation="MTRLSLATLLAAALFGPWSGCDREPVTPPAQPPAERAPEPAAVP
                     AELPGEGILLLPATRRLG"
     gene            complement(6207..>6503)
                     /locus_tag="VFL84_05285"
     CDS             complement(6207..>6503)
                     /locus_tag="VFL84_05285"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HET7608068.1"
                     /translation="SVTGAFLLIVVLVGTDILLSLIKGWLPRVDRLIEGQPLVILRDG
                     VPLRSRMRIERVDDEDILTAARELQGIDRLEDIERAVLERNGGISIVPRRRAGS"
BASE COUNT         1102 a         2196 c         2130 g         1075 t
ORIGIN      
        1 tctgggcagc acaacagcac gcggcgtagt tttcgcggtc gagcagcgcg tcgtcgctgc
       61 gggcgctcgt gagaacgaac atgccgggaa ccgactcgag ccgcgcgcgc cggaccggac
      121 ccgcgcgttc ccctttcttc gcaacctcga gctccaccgc tagatccacg acggctcgcc
      181 gtgtcgccgg ccccagcaag tagaaccgcc acggctcggt cagacgatgg ttgggcgccc
      241 agcgggcgac ttcgattgcg gcgaacagcg tgtccacgcc gaccgcgtcc ggcgcgaaga
      301 gatcgatcga cctgcgctcg cgcagcacgg acgcaaagtc ttcggcctgc gccgagctcg
      361 tttcttgcac gtaatcctcc agcacttcgc gcgctgcaac catagcacgg ccgcgcgccg
      421 caagcgccgg agccattcag ttcatcgcca gcgcgacttg ctgcagcgcg aagcgacgag
      481 caaagaattc gtacgccgcg gtacgcacct tcgggaagta gaagaccgac gtgcggtgcc
      541 ccgtcagcaa atacatcgac tccggcgagc ccatcgtctc gcgcaattgc tcttgagctt
      601 tgaacgggat gatcgcgtcg gtgcgcgtca ggaccatgaa cactcgttcg gcgtcgatgt
      661 acggcgcaac gtcgagcgga tcgagcttca tggcggcgcc gagcttctcg ccgagcgcct
      721 cgcggctcgt gccgagctcg ctcgccatct cgtcgatcga gcgcacgacg cggcggtaat
      781 tcgtgttggc gaggaggtac ggcaagtcgc cgccggccat cgcgacgacg agcgaattca
      841 cgcgatgatc gagcgcggtc agcatgatgg catccatggc gccgaggctg actccgaaaa
      901 cgccgattcg cgaagcgtcg agctcgggct cgtgctcgat ccagtcgagc acgcggtgat
      961 agtcatgcaa gttgccgacc aacgcgctct cgggctcggt cagagcgtcg agcggatcgc
     1021 gcccccttgt cacgacgacg gccgcccacc cttgcttcgc gaagtagcgt gcgaagaagc
     1081 gcgggatcat gagctcgccg ttgaaaatcg gcagcagcat gacgacggga gtgcgcgcat
     1141 caccgtcgac gtcgtagtac tcgaagtcga tcgacgcggc cgggtctgtc ggggacggca
     1201 gcgaaagttg gcgcaccacg aaatgggcgc gtgttcgccc ctcgccgtcc tcttcggtct
     1261 cgagcggcgc cgactcgagg tcgtcgtaga cgagatcgtc gtagacgctc ggagtgcccg
     1321 cgggccaggt gtcgggaccg cgatagtcgt ccgagagcgg aacgtgcgtc gtgcaggctg
     1381 cgaacgtgat tagcgcgatc gatatggcgg tcgtgcgaat ggcgcttcct gtcgccgggg
     1441 aactcatcgt tgctttcagc ctccttgctc ccatcgtact aaaccgcgcg gcgctgcgcg
     1501 caatcgcacg ttccgcctac gcgcgggccc gactcgagcc ggcgcgagtt ttacctaagt
     1561 agaaagatgc aagaatggcg ccgcgtggcc gccgcgccgg cgctcatgcc gcgagccttg
     1621 gcgtcagccg gtagacgaag cccggcggca ggttcagcgg cgcgatgccg agcaggacgc
     1681 tgtcgacgcg cagcagcgcg cgcagatcgc gatcgacggg gcaacggccg gcgtacgtga
     1741 actggtgcag ccggccttgc ggcttcaaga gacgcaacgc ctgctgcagg attcggtagc
     1801 gcttctcggg cgagaagcag agcaatggca gcccgctgat cacgaagtcg aacttctgtg
     1861 cgtcgagcgc cgaatcgagc tcgagcgcgt cggcggccac gacgtggcag cgcggaaagc
     1921 gccgctcgag gagctgcacg aagtgcgggt cgcgctcgac gagatacaaa ttccccgggg
     1981 ctacgccgct cgcgagcagc gcggccgtga cgcggcccgt gccggcgccg agctcgacga
     2041 ccctcgagcc cacgccgacg tccctcgcca tgagcttggc cagcgacttg ccgctaggag
     2101 cgaatgcgcc tatcgcgagc ggattgcgaa tccagccctt caagaagtgg ccgaagtcgt
     2161 cgtctgacgt atgcgaatgc tgggactcgg acatgcgatc gccttcctcg cgaaaaagcg
     2221 gctaaagcac tgctggcgaa ccggggctac ttggcgcgga tccgcacgtt gcgaaacatc
     2281 acacggcccg cgccatactg cagagcgaac ggaccagctg ccaatttgtc gtcgttagtg
     2341 tcgacggtcg tctgtccgtt caatctcacg acgagatgcc gcccatcggc cgaaatctcg
     2401 tagtggttcc acttgtcgcc ggtctcgagc atgaccttcg gcgccgcgac ctcgacgatc
     2461 gcgccggtac ggtaagtcgg gtccggccgt tgatcgaaaa tgttgacctc gtagcaagtt
     2521 gcggcaccga tctcactcgg gttcgcgcag cggatgaaca cgccgctgtt cgcgtcgggc
     2581 gtgacccaga actcgagatc caggtcgaaa tccgcatagg acccggccgt cagcaggaat
     2641 cccgtgccgg aatccgcggc taccgcgccg tcctcgagct gccagttggc gtcgcctacc
     2701 tgcgtccagc ccgcgagact actgccgtcg aacaacgttt gccattcgcc gcccgccggc
     2761 ggtgccggtg ccgctacctc cggagtcccg ggcgagcagc ccgatgcggc gacgaccgat
     2821 agcgctacga cccagagctt cgctcgcatg aacccccccg agtaacggct gggcgacggc
     2881 ctgctggcgg cagccggctt gaatgcgcgg agcttagcag agcgcgcgcg gccgtgaaca
     2941 ctatcgagac acgcgccgtg tcttacggca tcggcggcgc ggcgggcgcg gccatcccgg
     3001 ccgcctcgta tgcgttcaat cggttgtaga gcgtgttcag gctgatgccg agagtttcgg
     3061 cggcggcgcg cttgtcgccg ttcaagtgct cgagcgtgcg catgatcagc tcacgctcga
     3121 cgtcgcggat cgagcggccg atcgcgagcc cttgttggcc gttcggctcg ttgaacgggc
     3181 tgtcgaaacg ctccggcaaa cgaagcaggg cttcgttcgg gtccgtcatg atggcggcgc
     3241 gcagcacgac gtgccgaagc tcgcgcacgt tgccgggcca gtgatagccg cgcagacgcg
     3301 ccgaatcctc ggccgagaat ctgcgcgttt cagggcccga tgcggtctgt gcctgaccca
     3361 agaaatggtc gacgagcaac ggcacgtctt gaatgcgcgc gcgcagcggc ggcagcgtga
     3421 ttggaaagac actcagccgg tagtagaggt cttcgcggag cgccttgtcg gcgattgctt
     3481 tcttcggatc gcggttcgtg gccgcgaccg tccgcgcgtc gatcggcacc tcgcgctcgc
     3541 tgcccaccgg caagaccgag cccgtctcga ggacgcgcag gaaatgcggc tggagctcga
     3601 ccggcatctc ggcgagctcg tccaggaaca acgtgcctcc gtcggcgcgc ttgaagaagc
     3661 ccgggtgccg gcggacggcg cccgtgaagc tgcccttctc gtggccgaac agctcgctcg
     3721 ccgcgagctc gcgcgacaac gagccgcagt tgacggcgac gaactgaccc ggccttccgc
     3781 tcgcgcggtg aatcgactcc gcgacgagct ccttgcccgt gcccgtctcg ccgagaatca
     3841 acacggggct cggcgtgcgg gcaaccttgt cgatttgctc gtacacggca agcatcgccg
     3901 gcgactcgcc gatgaggcag ccgaagtgca gattgccgtt cggcgtcgcg ctgccgtttt
     3961 gcgtgctccc atcggcctcg acggtgcgca ggagctcggt gctgccgatc ggcttcgtca
     4021 aatatgagac cgacggcccg ctcagattct tgatcagcga cttgataccg ggatgccccg
     4081 tgatgaacac gacgcgcttg accgggctcg cgcgcagctc ctcgagcaac tcgagcccgt
     4141 tgccgtcggg gagcatcagg tcgagcagca gcacgtcggg cgccgcgccg ccgagtagcc
     4201 ggcgcgcact ctcgaggcgc tccgcgctct cgacggtatg cccgtgcagc tgcagcattt
     4261 ctttcgcagc tgcgacaaag tcaggttcgt cgtctaccaa taagacgttt gccatgaata
     4321 ccccaccaag gacacatctt gcgtcgtggg ccgactcgcc ggccgctgct cgtggtcacg
     4381 agcctgaacg cattctaccc ctatcgatcc gtccaacgac aatcgcgcgc cgcgctcgtg
     4441 ttccgcgaag acgcgctaga gccgagaccc cgcgtaaaat aggacccgtg cgccctgcaa
     4501 acatctgcca tggtcggccc gttcccttgc ggcatgggcg acattatgtt gtttgcacgg
     4561 ccgatcatat ggttacactc gatacttgcg tgcttttcga catcgagcgc ttatgagtga
     4621 gctccaggac aacacgttct tgtcgtggtt gagccacgag ctgcgctcgc cgctgaacgc
     4681 gtgcgtgatg tgggtcgacg tcctcgcgct ctcgccgcag cccgacaagc ttgcgaaggc
     4741 cgtcgacgcg atcaagcgca acctcgcgcg acaggcgcgg ctcgtcaacg acttgaacga
     4801 cgctgcgaaa atttcctcgg gcggcatcga gctccgcacc gaaccgttcg acctggtcgc
     4861 actgctgcgg cacgagctcg acgcgtggca gtcgctcgct gcgggcaagc aactcgagct
     4921 tcaccctagc gtggagctcg actcggcgcc cgtcgtcggc gattccgcgc gcctcgcaca
     4981 agccgtgaac cacctggtcg agaacgccat tgcgagcacg ccgagcaacg gccgcgtcga
     5041 cgtgcgcgtg cacggcgcgg acggcaattg cgtggtcgag gtcgccgaca cgggcgccgg
     5101 gttgtcgacg gaggacgccg ccaatctggg cacgccgctc tggcgtgcat cgactacggc
     5161 gcgcgcgcgc gccggcatcg gcctcggtct tgcggtggct catcacgttg ccggcaagca
     5221 cggaggctcg ctgacggcga ccagcgcgcc gtcgggagca catttcgtgt tgacgctgcc
     5281 gctcgcacgg agcggcatcg attcggttgc gaagatggaa cgtacctcgg acctttcgga
     5341 cctctaaccc cgcgccgccc cgcggtccgc cccgtcctgc gggtgcggtt gccacgaagc
     5401 cggctcccaa ccttcgagtc ggtagtagcc ttcctcggaa ccatagccca cgcctacgat
     5461 ctcgggagga tccgggacgg gcggcggcat ttctggaatc ttcggctcac ccgggtcgac
     5521 gggatcgtcg acgaccggta gctccggctc gcgcggcgcg ctcggatcgc gatgattcgt
     5581 cggctccagc atggcttagg gttcctccca ccaaaggcga gtgcaaagtg cgtgccagcg
     5641 cagacggcgc cggttacggc cggccggaag cgcccaaagc ccttacattt tctcgaactt
     5701 cctctcaaaa ccttcgcggt acgcgtcctc gcgctcggcg agaacggccg ccctcgcagc
     5761 gctgatggtt ggcacgcggc ttgcctattg gtggtaggga ggaggtctac cgccatgaac
     5821 aacgagatcg agcctgacga cgacagctac gaacccgccg gcaaaggcgc cgcccgccgg
     5881 cgcgtcgacg cctaccacga agtgcttcgc gagcgagctg caatgctcga gctcgacgcg
     5941 ccgttcgtcg aaaccaggag ctggccgccg aggcgctact agccttcgac ggccccgcca
     6001 tgacgcgctt gtcgctcgcc acgttgctcg ccgcggcgct cttcggaccg tggagcggct
     6061 gcgaccgcga gcccgtgact ccgccggccc agccgccggc cgaacgggct cccgagccgg
     6121 ccgccgttcc ggcggagctg ccgggcgaag gcatcctgtt gctgcctgcg acgcgccgtc
     6181 tcggctgatc cgaaacgctc cgtgcgctaa gagccggcgc ggcgccgcgg cacgatcgag
     6241 atgccgccgt tgcgctcgag caccgcgcgc tcgatgtcct cgagccggtc gatgccctgc
     6301 aattcgcgcg cggccgtcag gatgtcttca tcgtcgacgc gctcgatgcg catgcgcgag
     6361 cgcaacggca cgccgtcgcg caagatcacg agcggctgac cttcgatgag ccggtcgacg
     6421 cgcggcagcc agcccttgat cagggacagc aagatgtccg tgccgacgag cacgacgatc
     6481 agcaggaaag cgcccgtgac cga
//
LOCUS       DASSNN010000290         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1438574, whole genome shotgun sequence.
ACCESSION   DASSNN010000290 DASSNN010000000
VERSION     DASSNN010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438574"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VFL84_05290"
     CDS             complement(<1..707)
                     /locus_tag="VFL84_05290"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7608069.1"
                     /translation="MPRERRFDLAAYALAAEIWGNPGERPIIASHGWLDNAGSFSLLA
                     PLLPGNEIAALDLAGHGKSGSRSADSAYNLWQDAGDLLDVADALGWRRFTLLGHSRGA
                     GISMLFAGAFPERVDKLVLLEGGLPLTAEPGEAPAGLAQALLEARALRDKGGRVFTDR
                     KTAIAERASGFSKVTTAAAEILARRSLREVPGGYQWHADQRLKAQSELRLTPEQARAF
                     ARRITAPVLLLFAERSP"
     gene            complement(714..2246)
                     /locus_tag="VFL84_05295"
     CDS             complement(714..2246)
                     /locus_tag="VFL84_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7608070.1"
                     /translation="MAPAVYDSAPVETRTIQVTVDAAGVIEPESTVEVKSKASGEVLA
                     VHAETGDVVQAGALLVEIDKRTPRNSLSQTEATLSAAQARRQIAQTQMERAQTLFKSQ
                     TLTQADVEQTQLEFANAESQVVSAKVAVENARITLEDTDVRAPITGTIIEKTVEPGTV
                     IASTTQNVSGGTTLMKMADLTSVQVRTRVDETDIGKIQPGMRTKVVVAAYPNQPFEGE
                     VLKIEPQALVEQNVTQFSVLIKLANRGGLLKPGMNAEVQIQIANREGVPAVPTAALRA
                     DTDVPLTAVMLGLDEAALRKELWPEGAPAGAKAKNTVTIGNRIIELPEGVDAGKFTAL
                     MQKRQSGQELTAEERTLMRSVFQQGGGNFAGGGNFGGGGNFGGGGPPGGGNFGGGGGN
                     FRGPPGSGGGNFFGGGPAQTQAPVARIPDYQFGGEYWVVALRGDQPVPIAVKTGLTDL
                     EYSEVVSGLEPGDRVLLLPSTSLYEQQERLQEFINQRFGSTTPFQQPNQQQQNQARFL
                     FR"
     gene            complement(2315..>2535)
                     /locus_tag="VFL84_05300"
     CDS             complement(2315..>2535)
                     /locus_tag="VFL84_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005367656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding protein"
                     /protein_id="HET7608071.1"
                     /translation="EPSILLADEPTGNLDSTTSRDIMALFDTLNTAGHTIVVVTHEHD
                     IAAHAKRIITFRDGMVAEDIINNERTTH"
BASE COUNT          370 a          859 c          860 g          446 t
ORIGIN      
        1 aacgggctcc gctcggcgaa caggagcagc acgggcgccg tgatgcggcg cgcgaacgcg
       61 cgcgcctgct caggcgtaag gcgcagctcc gattgcgcct tgagccgttg gtccgcatgc
      121 cactgatagc cgcccggcac ctcgcgcaac gagcggcgcg cgagtatctc ggccgccgcc
      181 gtcgtgacct tcgagaaccc gcttgcgcgc tcggcgatcg cggtcttgcg gtcggtgaag
      241 acgcggccgc ctttgtcgcg cagcgcgcgc gcctcgagca gcgcttgcgc gaggcctgcg
      301 ggcgcctcgc ccggctcggc cgtcagcggc aggccgccct cgagcagcac gagcttgtcg
      361 acgcgctcgg gaaacgcgcc cgcgaacagc atcgaaatcc cggcgccgcg cgagtggccg
      421 agcagcgtga agcgccgcca gcccagcgcg tcggcgacgt cgagcaaatc accggcgtct
      481 tgccagaggt tgtaggcgga gtcggccgag cgcgagccgc tcttgccgtg gcccgcgaga
      541 tcgagcgcgg cgatctcgtt gcccggcagc agcggcgcca gcaacgagaa gctgccggcg
      601 ttatcgagcc agccgtgaga agcgatgatc ggccgttcgc ccgggttgcc ccagatctcg
      661 gccgcgagcg cgtacgcggc caggtcgaag cggcgctcgc gcggcacggt ttgtcagcgg
      721 aacaggaagc gggcctggtt ctgctgctgt tgattgggct gctggaacgg cgtcgtcgag
      781 ccgaagcgct ggttgatgaa ttcctgcaag cgttcctgct gctcgtagag gctcgtgctc
      841 ggcaaaagca ggacgcgatc gccgggctcg aggcccgaga cgacctcgct gtactcgagg
      901 tccgtcaggc cggtcttgac cgcgatcggc acgggctggt cgccgcgcag cgcgacgacc
      961 cagtactcgc cgccgaactg ataatcggga atccgcgcga ccggcgcttg cgtttgcgca
     1021 gggccgccgc cgaaaaagtt gccgccgcca ctgcccggag gaccgcggaa gttgccgcca
     1081 ccgccgccga agttgccgcc gcccggaggc ccgccgccgc cgaagttgcc gccgccgccg
     1141 aagttgccac cgccggcgaa atttccgccg ccctgctgga agacgctacg catgagcgtg
     1201 cgctcttcgg ccgtcagctc ttggccggac tggcgctttt gcatgagcgc cgtaaacttg
     1261 ccggcgtcga cgccttccgg cagctcgatg atgcggttgc cgatcgtgac cgtgttcttc
     1321 gctttcgcac ccgcgggcgc gccttcgggc cagagctcct tgcgcagcgc cgcctcgtcg
     1381 agcccgagca tgaccgcggt cagaggcacg tcggtgtcgg cgcgcagcgc cgcggtcggc
     1441 acggccggaa caccctcgcg attcgcgatc tggatttgca cctcggcatt catgccgggc
     1501 ttgagcagcc cgccgcggtt cgcgagcttg atcagcaccg aaaactgcgt gacgttctgc
     1561 tcgacgagag cctgcggctc gatcttcaag acctcgcctt cgaacggctg attcggatac
     1621 gccgcgacga cgactttcgt gcgcatgccc ggctggatct tgccgatgtc ggtctcgtcg
     1681 acgcgcgtac gcacctggac gctcgtgaga tccgccatct tcatcaacgt cgtgccgccg
     1741 ctgacgtttt gcgtagtcga cgcgatgacc gtgcccggct cgaccgtctt ctcgatgatc
     1801 gtgcccgtga tcggcgcgcg gacgtcggtg tcctcgagcg tgatgcgcgc gttctcgacg
     1861 gcgaccttcg cgctgacgac ctgcgattcc gcgttcgcga actcgagctg cgtttgctcg
     1921 acatctgcct gcgtcagcgt ctgcgacttg aacagcgtct gcgcgcgttc catctgcgtc
     1981 tgcgcgatct gccggcgcgc ctgggcggcc gacagcgtcg cctccgtctg cgagagggaa
     2041 ttgcgcggcg tgcgcttgtc gatctcgacg agcaaggcgc cggcttggac gacgtcgccc
     2101 gtctccgcgt gaaccgcgag cacttctccg gacgctttcg acttgacctc gacggtcgac
     2161 tcgggctcga tgacgccggc ggcgtcgacg gtgacctgga tcgtgcgcgt ctcgacggga
     2221 gccgaatcgt agacggcggg cgccacgact tgcggatcct tatcgcaacc ggccaataga
     2281 gcgaccggaa ttatccagta gagactgcgc atagtcagtg agtcgttcgt tcgttgttga
     2341 tgatgtcttc cgcgaccatg ccgtcgcgga acgtgatgat gcgtttggcg tgcgcagcga
     2401 tgtcgtgctc gtgcgtgacg acgacgatcg tgtgtccggc cgtgttcagc gtatcgaaca
     2461 gggccatgat atcccggctc gtcgtcgaat cgaggttgcc cgtcggctcg tccgccaaaa
     2521 gaatgctcgg ctccg
//
LOCUS       DASSNN010000291         5189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_559158, whole genome shotgun sequence.
ACCESSION   DASSNN010000291 DASSNN010000000
VERSION     DASSNN010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559158"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..3328)
                     /gene="treS"
                     /locus_tag="VFL84_05305"
     CDS             complement(44..3328)
                     /gene="treS"
                     /locus_tag="VFL84_05305"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HET7608072.1"
                     /translation="MSTDASDDPLWYKDAVIYQLHVRSFFDQNDDGVGDFAGLTSKLD
                     YIERLGVSAIWLLPFYPSPMRDDGYDIADYLGINPVYGTLSDFQAFVRAAHERGLKII
                     TELVVNHTSDQHPWFQAARTAPRDSERRQLYVWSDTDKRFPETRIIFTDTEQSNWAWD
                     PVAGQYYWHRFFSHQPDLNHNNPAVVNAVIRVMQHWLDLGVDGLRLDAIPYLCVRDGT
                     SNENLPETHAVVKRFRRAIDARYKNRMLLAEANQWPEDVREYFGDGDECHMAYHFPLM
                     PRMYMAIAQEDRYPIVEILQQTPDIPPTCQWAIFLRNHDELTLEMVTDRERDYMYQMY
                     AADPRARVNVGIRRRLAPLMENDPDKIQLMKSLLFSMPGSPTVYYGDELGMGDNIYLG
                     DRNSVRTPMQWSPDRNGGFSRADPQSLYLPPIMDPVYGYASVNVEAQSREPSSLLNWT
                     RRMLAVRKTCKAFGRGTFELLRPGNRTILAYVRRYENQAVLCVANLKRSAQAVELNLS
                     QFRGRVPVEMIGRSSFPPIGELPYMLTLPGYAFYWFDLAESAPPPSWHEERLPADERP
                     WLVLFNGLDSFQVDGGERQGAAAKLLAQLEREVIPSYLATQRWFAGKGSGEPTIKITE
                     IGRWQQKQQVWLLLIASAQFPADEPQEYVIPLALEWGAERAGPHAAHTLARVRQRAAT
                     GILFDAFADDDFVRALLAALSERASVRVGAGTLRFTPTTALGALLPKRLAQAPVRPAA
                     EGSNLSFVVGEQLFLKAYRRLRAGAHPEWELGRFLTERSPCSAVAQTAGAIVLDRPSG
                     EPATVALVQAAVRNQGDGWKYTLRYLERSLDDAVARATDGNVGSGDHTSYLLLVRTLA
                     ARTADLHRALAVKTGDPSFDPEPLTPAHVAEWAAAIAAELDTTLSSLRARRSELPDEA
                     REVAGQALAAAPRLRGAISKHAAGPTTALAIRLHGDYHLGQVLITRNDFVITDLEGEP
                     GRSLEERRRKHTAVKDLAAMGRSFDYARVVAAQQFATKPGSGAADVRAFLDDWRAQVH
                     LAFREAYSEAIGDSAVLPRDAAEEERLRALATIERLLYEIRYELGNRPELVTVPLLDL
                     TALLEE"
     gene            complement(3334..>5189)
                     /locus_tag="VFL84_05310"
     CDS             complement(3334..>5189)
                     /locus_tag="VFL84_05310"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HET7608073.1"
                     /translation="HERIACALLHAAPGKESWSVVPMAPLREDVWQATFVPAELGRYR
                     YTVAAWVDAFATWRHKFERRTQRDDIELALLEGAGLVRAAAARARGAERGALLAYAAS
                     LESDAPLEERRQSALTEALRELMASSPDRSLETVYDPPLEAVVERPLARFGSWYEFFP
                     RSAAEPPARHGTFVTAARHLAYIAAMGFDVVYLPPIHPIGSSHRKGRNNSLEAGADDP
                     GSPWAIGGTDGGHKSVHADLGTLEDFVGFRREAERLKLEVALDIAFQCSPDHPYVREH
                     PLWFARRPDGTVQFAENPPKKYEDIYPIDFGTAEWRALWTELRDVVLFWIDHGIKIFR
                     VDNPHTKPFDFWRWLIADVQGRHPDTIFLAEAFSRPRVMHRLAKLGFSQSYTYFTWRN
                     TKQELTQYFTELKTMRDWFRPNLWPNTPDILHEYLQSGGRPAFMARAALAATLAASYG
                     IYGPAFELLEHTPREAGSEEYRDSEKYEVKRWDLARADSLKSFLTRLNTIRRENAALQ
                     HDDTLTFRRIDNDRLICYSKTSHDGKNIVLVAVNLDPQHAQSGFIDVPLEEWSLHAHE
                     AYYAEELLSGQRYEWQGSRVFVTLSPAECPACVFRIEARRAASERDFDYFV"
BASE COUNT          813 a         1716 c         1809 g          851 t
ORIGIN      
        1 gtcagggacg ccacggccgg ccgcgccgga cgcgaggccg cggctactcc tcgagcaagg
       61 cggtgaggtc gaggagcggc acggtcacga gctcgggcct attcccaagc tcgtaccgga
      121 tctcgtagag caagcgctcg atcgtcgcga gcgcgcgcag ccgctcttcc tccgcggcat
      181 cgcgcggcag caccgcgctg tcaccgatcg cttcgctgta ggcctcgcgg aacgccaggt
      241 ggacctgcgc gcgccagtcg tcgaggaacg cgcgcacgtc ggcggcgccc gagccgggct
      301 tcgtcgcgaa ctgctgcgcg gccacgacac gcgcgtagtc gaacgagcgg cccatcgccg
      361 ccaaatcctt gacggccgtg tgcttgcgcc ggcgctcctc caagctgcgg cccggctcgc
      421 cctcgagatc ggtgatcacg aaatcgttcc tcgtgatcag gacctggccg aggtgatagt
      481 cgccgtgcag gcgaatcgcg agcgccgtcg tcgggcccgc cgcgtgcttc gagatcgcgc
      541 cgcgcaggcg cggcgccgcg gcaagcgctt gccccgcaac ctcgcgagcc tcgtcgggaa
      601 gctcgctgcg ccgcgcgcgc aagctcgaca gcgtcgtgtc gagctcggcg gcgatggcgg
      661 cggcccattc ggcgacgtgc gccggcgtga gcggttcggg atcgaagctc gggtcgccgg
      721 tcttcacggc gagcgcgcgg tgcaagtcgg ccgttcgcgc ggcgagcgtg cgcacgagca
      781 gcaagtagct cgtgtggtcg ccgctgccga cgttgccgtc cgtcgcgcgc gccacggcgt
      841 cgtcgagact gcgctcgaga tagcgcagcg tgtacttcca gccgtcgccc tgattgcgga
      901 cggcagcttg cacgagagcg accgtcgccg gctcgcccga gggccggtcg aggacgatcg
      961 cgcctgccgt ctgcgcgacg gcgctgcacg gcgaacgctc cgtgagaaag cggccgagct
     1021 cccactccgg gtgcgcgccg gcgcgcaggc gccgatacgc tttgaggaac agctgctcgc
     1081 cgacgacgaa gctcaagttg ctcccctcgg cggcgggccg aaccggcgcc tgcgcgagcc
     1141 gcttcggaag cagcgcgccg agcgcagtcg tcggcgtgaa acgcaaggtt cccgcgccga
     1201 cgcgcaccga agcgcgctcg ctcagggcgg cgagcaacgc gcgcacgaag tcgtcgtcgg
     1261 cgaacgcgtc gaacaggatt cccgtcgcgg ctctttgccg cacgcgcgcc agcgtatggg
     1321 ccgcatgcgg cccggcgcgc tcggcgcccc attcgagcgc gagcgggatc acgtactcct
     1381 gcggctcgtc ggccgggaac tgcgcgctcg cgatcagcag cagccacact tgctgcttct
     1441 gctgccagcg gccgatctcg gtgatcttga tcgtcggctc gccggacccc ttgcccgcga
     1501 accagcgctg cgtcgcgaga tagctcggga tcacctcgcg ctccagctga gcgagcagct
     1561 ttgccgccgc gccctggcgc tccccgccgt cgacctggaa gctgtcgagc ccgttgaaca
     1621 gcacgagcca cggccgctcg tcggccggca agcgctcctc gtgccagctc ggcggcggcg
     1681 cgctctcggc aaggtcgaac cagtagaacg cgtagcccgg cagcgtcagc atgtagggga
     1741 gctcgccgat cggcgggaac gacgagcggc cgatcatctc gacgggcacg cggccgcgga
     1801 attgcgagag attcagctcg acggcttgcg cggagcgctt caagttcgcg acgcagagga
     1861 cggcctgatt ctcgtagcgg cgcacgtaag cgaggatcgt gcgattgccc ggccggagca
     1921 gctcgaacgt gccgcggccg aacgctttgc aggttttgcg gacggcgagc atgcgccgcg
     1981 tccaattgag cagcgacgac ggctcgcgcg attgggcctc gacgttcacg gaggcatacc
     2041 cgtagaccgg atccatgatc ggcggcagat agagactctg cggatcggcg cgcgagaacc
     2101 cgccgtttcg atccgggctc cactgcatcg gcgtgcgcac gctgttccgg tcgcccaagt
     2161 agatgttgtc gcccatgccg agctcgtcgc cgtagtagac ggttggcgat cccggcatcg
     2221 agaacagcag gctcttcatg agctggattt tgtcgggatc gttctccatg agcggcgcga
     2281 gccgccgcct gatgccgacg ttgacgcggg cgcgcggatc ggccgcatac atctggtaca
     2341 tgtagtcgcg ctccctatcg gtgaccatct cgagcgtcag ctcgtcgtgg ttgcgcagga
     2401 agatcgccca ctggcatgtc ggcggaatgt cgggcgtctg ctgaagtatc tcgacgatcg
     2461 ggtagcggtc ttcctgtgcg atcgccatgt acatgcgggg catcagcggg aagtggtacg
     2521 ccatgtggca ctcgtcgccg tcgccgaagt attcgcgcac gtcttcaggc cactggttgg
     2581 cctcggcgag cagcatgcgg ttcttgtagc gcgcgtcgat ggcgcgtctg aaacgcttca
     2641 cgaccgcgtg ggtctccggc aggttctcgt tcgaggtgcc gtcgcgcacg cacaggtacg
     2701 gaatcgcgtc gagcctgagc ccgtcgactc cgaggtcgag ccagtgctgc atgacgcgga
     2761 tcacggcgtt cacgacggcc gggttgttgt ggttcaagtc cggctggtgc gagaagaagc
     2821 ggtgccagta gtactggccg gcgacggggt cccaagccca gttcgattgc tcggtgtccg
     2881 tgaagatgat gcgcgtctcg gggaatcgct tgtcggtatc gctccagacg tacagctggc
     2941 ggcgctccga atcgcgcggc gccgtgcgcg ccgcttggaa ccacggatgc tggtcggacg
     3001 tgtggttcac gacgagctcg gtgattatct tgagcccgcg ttcatgcgcg gcgcgcacga
     3061 acgcctgaaa gtcgctcaac gtgccataga cgggattgat gccgagatag tcggcgatgt
     3121 cgtagccgtc gtcgcgcatc ggcgaggggt agaacggcag cagccagatc gcgctgacgc
     3181 cgagccgttc gatgtagtcg agcttcgagg tcaggccagc gaaatcgccg acgccgtcgt
     3241 cgttctgatc gaagaacgac cgcacgtgca gctgatagat gacggcgtcc ttgtaccaga
     3301 gcgggtcgtc gctcgcgtct gtgctcatca ggtttacacg aagtaatcga agtcgcgttc
     3361 cgatgcagcc cggcgcgctt cgatgcggaa cacgcaggcc gggcactcgg ccggagacag
     3421 cgtgacgaaa acgcgcgaac cttgccactc gtaacgctga cccgacagca gctcctcggc
     3481 gtaatacgcc tcgtgggcgt ggagcgacca ttcctcgagc ggaacgtcga tgaagccgct
     3541 ttgcgcatgc tggggatcga gattcacggc gacgagcacg atgttctttc cgtcatggct
     3601 cgtcttcgaa tagcagatga gccgatcgtt gtcgatacgc cgaaacgtca gcgtgtcgtc
     3661 gtgctgcaga gcggcgttct cgcgccggat cgtgttgagg cgcgtcagga aggacttcag
     3721 cgagtccgcg cgcgcgaggt cccagcgctt gacctcgtac ttctccgaat cgcggtactc
     3781 ctcgctgccg gcctcgcgcg gcgtgtgctc gaggagctcg aacgcggggc cgtagatgcc
     3841 gtagctcgcg gcgagcgtcg ccgcgagcgc ggcgcgcgcc atgaaggcag gccggccgcc
     3901 gctctgcaaa tactcgtgca ggatgtcggg cgtgttcggc cagagattgg gccgaaacca
     3961 gtcccgcatc gtcttgagct cggtgaagta ctgggtcagc tcctgcttcg tgttgcgcca
     4021 cgtgaagtac gtgtaggact ggctgaaccc gagcttcgcg agccgatgca tcacgcgcgg
     4081 gcggctgaag gcttcggcca ggaaaatcgt atccgggtga cggccctgga cgtccgcgat
     4141 gagccagcgc caaaaatcga acggcttcgt gtgcgggttg tcgacgcgaa atatcttgat
     4201 gccgtggtcg atccagaaca ggacgacgtc gcggagctcc gtccacagcg cgcgccattc
     4261 ggcagtgccg aagtcgatgg gatagatgtc ttcgtacttc ttcggcggat tctccgcgaa
     4321 ttgcaccgtg ccatcggggc ggcgcgcaaa ccacagagga tgctcgcgga catacggatg
     4381 atcgggcgag cactgaaatg cgatgtcgag agcgacctcg agcttcaggc gctccgcttc
     4441 gcggcggaag cccacgaagt cctcgagcgt gccgaggtcc gcgtgaacgc tcttgtgccc
     4501 gccgtccgtg ccgccgatcg cccacgggga gcccggatcg tccgcgccgg cctcgagcga
     4561 gttgttgcgg cccttacggt ggctcgagcc gatcgggtgg atcggcggca ggtacacgac
     4621 gtcgaatccc atcgccgcga tatacgcgag gtggcgcgcc gccgtcacga acgtcccgtg
     4681 gcgcgcgggc ggttctgcgg cggaccgcgg gaagaactcg taccacgagc cgaaccgcgc
     4741 gagcggccgc tcgacgacgg cctcgagcgg cggatcgtag accgtctcga gcgagcggtc
     4801 cggagacgag gccatgagct cgcgcagcgc ttccgtcagg gcgctctgcc ggcgctcttc
     4861 gagcggcgca tcgctctcga gcgacgcggc gtacgcgagc agcgcgccgc gctcggcccc
     4921 tcgcgcgcgc gccgccgccg cgcggacgag cccggcgcct tcgagcagcg cgagctcgat
     4981 gtcgtcgcgc tgcgtcctgc gctcgaactt gtggcgccaa gtcgcgaacg cgtcgaccca
     5041 cgcggccacg gtgtagcggt agcggcctag ctcggccggc acgaaggtcg cctgccaaac
     5101 gtcctcgcgc agcggcgcca tcggcacgac gctccagctt tcctttccgg gcgcggcgtg
     5161 cagcagcgcg cacgcgatcc gctcgtggc
//
LOCUS       DASSNN010000292         2580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1297006, whole genome shotgun sequence.
ACCESSION   DASSNN010000292 DASSNN010000000
VERSION     DASSNN010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297006"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1499
                     /locus_tag="VFL84_05315"
     CDS             228..1499
                     /locus_tag="VFL84_05315"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HET7608074.1"
                     /translation="MTDGTADAYAAFRLNLEQQHKRAKDLLKRAKAGERAALERLRGA
                     GFASPAELKLAQAQHCIARELRFASWAALKRHIGEMERARQAPPEVLDGDCRTMHIRC
                     GTDFLRELREAGLRGDFNAHLNPYLQGPVTDTPDWLERRARFIAETFGPYDAQDYATA
                     LEGARDEERRLAAASREYERVVLWLEHDRYDQFVLLRCLAWFAEHGAPPRLELVGPSD
                     FPGSARFVGIGQLPPEALRLLWGRRQPLSAQQLAFGHRAWAAFRAPDPRELAALARAG
                     TPPLPALAGALHRHLQELPSPTDGLGLTHRLLLTALAEHGAQRVGRLVGLVMHGRDPL
                     PGLGDVGYDLALRELAAPPDPLVQRSGGLARGEWHRDEIAITATGLDVLEGKRDGLAL
                     PLPERWVGGVRVAPGQLNWRWDERARAVELR"
     gene            complement(1510..>2580)
                     /locus_tag="VFL84_05320"
     CDS             complement(1510..>2580)
                     /locus_tag="VFL84_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7608075.1"
                     /translation="FSARETQNEATSLQGVSGDHNFASWKVAGIYDPLDWLRLRATVS
                     RDVRAAGFRELFLPRTTVIGFATVTNPWFGGATDTSFAATAGGNPDLEPEEADTQTFG
                     AVFSFDRLRLSADWFEIDLANAITQSPGNQPLVNACFSSGGTGPACDRIVGEGTNDIT
                     AIDSGAMNLAGFLTRGWDYEATLDFPLSAGGNLNLRLIGTYLYDMIVDTGLGPKPIDY
                     EGQSGPVASFGSFNTQPKWQARAFLTYARDRFTSTVETRYVGSGSLNVTWTESPIGAP
                     NNNQLNTVNDNSVDDAYYLSWSGSYDFRRTEGNAVQLFWVINNLLDEEPVIAPGGNAY
                     PTNPVFFDTLGLRYRAGVRFQF"
BASE COUNT          388 a          908 c          873 g          411 t
ORIGIN      
        1 ccgtaacacc ggcacctttg ttgcgggcac ctttgttgac gccggcacct ttgttgacgc
       61 agccgttgac gcgctcggtc gaccggccgg acaatgcttt tcgctcggtc ggagcatcca
      121 cgtccgcgga aagggctgcg cccacgaccc tctcgtcgga gactcgctcc cacgccaagt
      181 ctcttttccg cctgattgcg gacgcaggcg cacgaaagga gacgttcatg accgacggca
      241 ctgcggacgc ctacgccgcg ttccgcctca atctcgagca acagcacaag cgcgcgaagg
      301 atttgctcaa gcgcgcgaag gccggcgagc gcgcagcgct cgagcgcctg cgaggcgccg
      361 gcttcgcatc gcctgccgag ctcaagctcg cgcaggcgca gcactgcatc gcgcgcgagc
      421 tgcgctttgc gagctgggcc gcgctgaaac gccacatcgg cgaaatggag cgcgcgcggc
      481 aggcgccgcc cgaggtcctc gacggcgatt gccgcacgat gcacatccgc tgcgggaccg
      541 acttcctgcg cgagctcagg gaagcgggcc tgcgcggcga cttcaacgcg cacctcaatc
      601 cgtatttgca ggggcccgtc accgacacgc ccgactggct cgagcggcgc gcgcggttca
      661 tcgccgagac gttcggcccc tacgacgcgc aggactacgc gaccgcgctc gagggcgcgc
      721 gcgacgaaga gcgccggctc gcggcggcca gccgcgagta cgagcgcgtc gtgctctggc
      781 tcgagcacga ccgctacgat caattcgtct tgctccgctg cctggcctgg ttcgccgagc
      841 acggagcgcc gccgcggctc gagctcgtcg ggccgagcga ctttcccggc agcgctcgat
      901 tcgtcgggat cgggcagctg ccgcccgaag cgttgcgcct tctctgggga cggcggcagc
      961 cgctgagcgc gcagcagctc gcgttcggcc atcgcgcctg ggccgcgttc cgcgcgccgg
     1021 atccacgcga gctcgccgcg ctcgcgcgcg ccggcacgcc gccgctgccc gcgctcgcgg
     1081 gcgcgcttca tcggcacctg caagagctgc cgtcgccgac ggacggcctc ggactcacgc
     1141 accgcttgct gctcacggcg ctcgcggagc acggagcgca gcgcgtgggc cggctcgtcg
     1201 gcctcgtcat gcacggccgc gatccgctgc cgggtctcgg cgacgtcggc tacgacctcg
     1261 cgctgcgcga gctcgcggcg cctcccgatc cgctcgtgca gcgcagcggc ggcctcgcgc
     1321 gcggcgagtg gcatcgcgac gagatcgcga tcacggccac cggcctcgac gtgctcgaag
     1381 gcaaacgcga cgggctcgcc ctgccgctgc ccgaacgctg ggtcggcgga gtgcgcgtcg
     1441 cgcccggcca gctcaattgg cgctgggacg agcgcgcgcg cgccgtcgag ctccgctgac
     1501 gctacgacgt cagaactgga aacgcacccc cgcgcggtag cgcaagccca gcgtgtcgaa
     1561 gaacaccggg ttcgtcgggt aggcgttgcc gcccggcgcg atgaccggct cttcgtcgag
     1621 caggttgttg atgacccaga acagctggac cgcattgcct tcggttcgcc tgaagtcgta
     1681 tgacccggac cagctcaggt agtacgcgtc gtcgacgctg ttgtcgttga cggtgttgag
     1741 ctgattgttg ttcggagcgc cgatcgggct ctcggtccaa gtcacgttga ggctgccgga
     1801 cccgacatac cgtgtctcga ccgtgctcgt gaaccgatcg cgcgcatacg tcaggaatgc
     1861 gcgcgcctgc cacttgggct gcgtattgaa cgagccgaac gaagcgaccg ggccgctctg
     1921 gccctcatag tcgatcggct tcggtccaag gccggtatcg acgatcatgt cgtagaggta
     1981 cgtcccgatg aggcgcaggt tcaggttgcc gccggcgctg agtgggaagt cgagcgtcgc
     2041 ctcgtagtcc caacctctcg tcaagaaccc ggctagattc atcgcgccgg aatcgatcgc
     2101 cgtgatgtcg ttcgttccct cgccgacgat tcgatcgcac gcggggcccg tgccgccgct
     2161 cgagaagcac gcgttcacga gcggctggtt gccggggctt tgcgtgattg cattggccaa
     2221 gtcgatctcg aaccagtcgg ccgagaggcg cagccgatcg aacgagaaga cggcgccgaa
     2281 cgtctgcgta tccgcttcct cgggctcgag atcggggttg ccgcccgcgg tcgccgcaaa
     2341 gctcgtgtcg gtcgcgcctc cgaaccaagg attcgtgacg gtggcaaagc cgattaccgt
     2401 cgtgcgcggc aggaacagct cacggaagcc ggctgcgcgc acgtcgcgcg acaccgtcgc
     2461 gcggagccgc agccagtcga gtggatcgta gatgccggcg accttccagc tcgcgaagtt
     2521 gtggtcgccc gagacaccct gcaagctcgt cgcctcgttc tgcgtctcgc gcgccgagaa
//
LOCUS       DASSNN010000293         8132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_433201, whole genome shotgun sequence.
ACCESSION   DASSNN010000293 DASSNN010000000
VERSION     DASSNN010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433201"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFL84_05325"
     CDS             complement(<1..296)
                     /locus_tag="VFL84_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HET7608076.1"
                     /translation="MAAVDIAQLLAFAVKNNASDLHLSSGVPPIIRVDGDVKRINMPP
                     LKHKEVHSMVYDIMNDKQRKDYEEFLETDFSFEIPKLARVRVNAFNQNRGAGAV"
     gene            379..1056
                     /locus_tag="VFL84_05330"
     CDS             379..1056
                     /locus_tag="VFL84_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET7608077.1"
                     /translation="MTEITDHLAEIFERVARAAERARRSPSSVTIVAIAKQQPASAIA
                     EAQRAGLANFGESYVQEALPKIAALADLPVSWHFVGKMQANKTRQVAEAFDWVHTVDR
                     LKIAERLSEQRSPFAAPLNVLIQVNQGGEPQKSGARPEDVPELARAIAALPRLTLRGL
                     MTLPPQSETPAHWFAKLAALRARLQSDGAALDSLSMGMSGDFEEAIAAGATHVRIGTA
                     IFGARAA"
     gene            1111..1689
                     /locus_tag="VFL84_05335"
     CDS             1111..1689
                     /locus_tag="VFL84_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET7608078.1"
                     /translation="MLQALTFLVRTLADLYLLTFLLRFIMQWSRANYYNPLSQLVFRV
                     TNPLVVPARRVMPSVAGLDVPTLVVLIVLEVIVTFVLLRLAGLSLPIPRLLFYSVLRL
                     LSLGLWFYIGALIIYAVLSWFGQPSRNPMAVLLGELVEPVLRPARRMLPPVGGLDLSV
                     LLVIVLLEAMNIIVASLKAATIASPILPAYLP"
     gene            1763..2260
                     /locus_tag="VFL84_05340"
     CDS             1763..2260
                     /locus_tag="VFL84_05340"
                     /inference="COORDINATES: protein motif:HMM:NF025822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HET7608079.1"
                     /translation="MVRRSRTLACLVIAAGIAACEPTGPVDATNTQEGVLPATEGSKD
                     FGEYVVYFNAIKTDQLAPDIAREYGIVRSKSRAMLNVSIHHKLPNGMTEAVEGAVSAS
                     AVNLNGQLKTMTLRKVPEENAIYYIGELGITDGEVLIYTIDVTPQGEASRFTVRFKKQ
                     FFVEE"
     gene            complement(2276..2725)
                     /locus_tag="VFL84_05345"
     CDS             complement(2276..2725)
                     /locus_tag="VFL84_05345"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HET7608080.1"
                     /translation="MLRKLAALFDDAFSPKADDPAAREQALRIATALLLIEVAKADYA
                     EEVAEDEVLLQSLKQFFALDDAAAALLLEEARSTADRAVELQQFTRRLHEQLSVAEKL
                     RVVEMLWQVALADSRLDKHEDHLVRRIAELLYVSHSDLIRIRNRVRS"
     gene            complement(2715..3386)
                     /locus_tag="VFL84_05350"
     CDS             complement(2715..3386)
                     /locus_tag="VFL84_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7608081.1"
                     /translation="MSQSTARAPRSASIEIPGPAGVLEAAIDASVGPAVATAVVCHPH
                     PQQQGTMTNKVVTTLARAFARLNADAVRFNFRGVGESAGAYAGGTGEGDDALAVVAWC
                     RERWPARPLYLGGFSFGAAVAASIAARVEPAGLVTVAPPVERLRAGFAAPRCPWLLIH
                     GDKDDVVPPGPVLEWASALPSPPKIVLLPGVGHFFHGSLAAITRAVTETFGPELESYG
                     RSNAS"
     gene            complement(3383..5260)
                     /gene="htpG"
                     /locus_tag="VFL84_05355"
     CDS             complement(3383..5260)
                     /gene="htpG"
                     /locus_tag="VFL84_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HET7608082.1"
                     /translation="MVSGEPSTFAFKAEVKQLLHLMVHSLYSEREIFLRELISNASDA
                     NDRLRFESLARPELSAAEPELGILVTVDPEQRTLSVTDSGIGMSRDEVIDNLGTIARS
                     GTGQFLEMLTGDQRKDAQLIGQFGVGFYSAFIVADEVTVLSRRADAPPEEGVSWISDG
                     RGEYRIETVERPARGTTVQLKLKENAAEFLEPERLKALIRKYSDHIAFPVRLTGGGHE
                     EETVNRAKALWARPRAEIKDDEYVEFYMALAHDSEEPLIWSHNRVEGKREYTSLLYVP
                     ATAPFDLWNREAPRGVKLYVQRVFITDQATQFLPLYLRFIRGVVDSSELALNVSRELL
                     QQDEAIGAMRAALTRRVLDMLERLAEDSTKYAKFWSQFGALLKEGLIEDVANRERIAK
                     LMRFGTTKTASDEQSRSLEQYLSDAKPDQEAIYFLVAESATAARTSPHLEAFKERGIE
                     VLLLTDRLDEWVMQHLTQHGGKPFKDVRRGVLDFDAAKDGAPKAKPQDREQQALLKRV
                     KQVLRERIDEVRVGGRLRQSAACLVLNEHDLGYQMRELLSAAGHKAPAAIPSLELNPE
                     HALVKRLDAESDPGRFERLAFLLFEQAVLAEGRQLEDPAAFVARLNELLVDLGAQSGR
                     G"
     gene            complement(5316..5582)
                     /locus_tag="VFL84_05360"
     CDS             complement(5316..5582)
                     /locus_tag="VFL84_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608083.1"
                     /translation="MEVVLLWLDDLDDALFSVALAWERLRRLVLQVGLSSAIGFAATQ
                     LAAIATAWAPALWYVAAASVVAWLAGTLLRVVYHREARRFLTTA"
     gene            5937..6704
                     /locus_tag="VFL84_05365"
     CDS             5937..6704
                     /locus_tag="VFL84_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET7608084.1"
                     /translation="MQRIVVLNPKGGSGKTTIAINLAACYAANGGRPALMDLDPQASC
                     TRWLRKRPDEAAPIHGIAAFERSPSVTRSWQTRVPADCGVVVIDTPAAIDPLALPDLT
                     RGADAVLVPVMPSDIDIHATAKCIADLLLVAKIRRSQKRIGIIANRVRGNTRVSQSLT
                     RFLNSLDIPLIATLRDTQSYVRSAETGLGVCEMPRWQVGQDLTHWREMLAWLTARPGS
                     QPAAGSPTNLLSLERPSPQPDRSPLASAAGRAGAARD"
     gene            complement(6715..6912)
                     /locus_tag="VFL84_05370"
     CDS             complement(6715..6912)
                     /locus_tag="VFL84_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608085.1"
                     /translation="MYSKEQNGADEPRSNDQHGWDAYRKWLSRVSTKSAGERTPLDHS
                     IYSWRGYHTWADKVRQSWKDG"
     gene            complement(7073..>8132)
                     /gene="ppnN"
                     /locus_tag="VFL84_05375"
     CDS             complement(7073..>8132)
                     /gene="ppnN"
                     /locus_tag="VFL84_05375"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HET7608086.1"
                     /translation="LRDLLYAHENVLKNPTFDLKTTDGLTDAVFHILRNARVIKAHED
                     PRLVVCWGGHAIEREEYDYTKVVGYALGLQGLDICTGCGAGAMKGPMKGATVGHAKQR
                     IRNGRYLGLTEPTIIAAESPNPICNELVILPDMEKRLEAFVRMAHAIIVFPGGVGTAE
                     EILYLLGILLDPKNSGETLPVIFTGPPSSKSYFERLHSFIGATLGPVAQQRYKVILGD
                     PPAVAAEVTSGLRRVRDRRAADDNAYFFNWALEIGLEFQKPFEATHESMRALELHRDQ
                     PAHSLAVNLRRAFSGIVAGNVREAGIALVEKYGPFELRGDPKIIGALGTLLQGFVTER
                     RMRLTDPSAYVPCYRIVA"
BASE COUNT         1365 a         2677 c         2660 g         1430 t
ORIGIN      
        1 aacacggcgc cggcgccgcg gttctggttg aacgcgttca cgcggacgcg cgcgagcttc
       61 gggatctcga acgagaagtc agtctccaag aactcttcgt agtctttgcg ctgcttgtcg
      121 ttcatgatgt cgtacaccat gctgtgcact tctttgtgct tcagcggcgg catgttgatg
      181 cgcttgacgt cgccgtcgac gcggatgata ggagggacgc ccgatgacaa gtgcaggtcc
      241 gaggcgttgt tcttgacagc aaaagcaagc agctgcgcaa tatcgaccgc ggccatagca
      301 ctccctttag cacgggcccg tagccctcgt gagaagggct gagaagttga cagtatagcc
      361 aagggctgcc ggcaaagcgt gactgaaatc acggaccatt tggcagaaat cttcgagcgt
      421 gtcgcgcgcg cagcagagcg tgcacgacga tctccgagca gcgtcacgat cgtggcaatc
      481 gccaagcagc agcccgcgtc cgcgatcgcc gaggcgcagc gcgcggggct cgcgaatttc
      541 ggcgagagct acgtgcaaga agcgctgccg aagatcgcgg cgctcgccga tctaccggtc
      601 agctggcact tcgtcggcaa gatgcaagca aacaagactc ggcaagtcgc ggaagctttc
      661 gattgggtac acaccgtcga tcgcctcaag atagcggagc ggttgagcga gcagcgcagc
      721 ccgttcgcgg cgccgctgaa tgtgctgatt caggtcaacc agggcggcga gccgcagaag
      781 agcggcgcgc ggcccgagga cgtcccggaa ttggcgcgcg cgattgccgc gttgccgcgc
      841 ctcacgctgc gaggcctcat gacgctgccg ccgcagagcg aaacgccggc gcattggttc
      901 gcgaagctcg cggcgctgcg cgcgcggctg cagagcgacg gagcggcgct cgacagcctg
      961 tcgatgggaa tgtcgggcga cttcgaggaa gcgatcgccg ccggtgcgac gcacgtgcga
     1021 atcggcaccg cgatcttcgg ggcccgcgcc gcctagccgt gtagcgcccg cctcgtgcta
     1081 tcttcgcgcg catcgatcga gtagctaccg atgcttcaag cgctgacttt cctcgtccga
     1141 actcttgccg acctctatct gctgacgttc ctgctgcgct tcatcatgca gtggtcgcgc
     1201 gcgaactact acaacccgtt atctcagctc gtattcagag tgacgaaccc gctcgtcgtg
     1261 ccggcgcggc gagtgatgcc gagcgtcgcg ggcctcgacg tgccgacgct ggttgtcctg
     1321 atcgtcctcg aggtcatcgt caccttcgtg ctgctgcggc tcgccggctt gtcgttaccg
     1381 ataccgcggc tccttttcta ctccgtgctg cggctgctat cgctcgggct ctggttctac
     1441 atcggcgcgc tcatcatcta cgcggtgctc agctggttcg ggcagccgag cagaaatccg
     1501 atggccgtgt tgctcggcga gctcgtcgag ccggtgttgc gccccgcgcg gcgtatgttg
     1561 ccgccggtcg gcggtctcga tctttcggtg ctgctcgtga tcgtgctgct cgaagccatg
     1621 aacatcatcg tggcctcgtt gaaagcggcc acgattgcct caccgatcct gccggcctac
     1681 cttccctaga cagtgctcga gcacgcgcgt aatgtcgagc gtgcgccaac tttgggctag
     1741 cgcatcacgg gagtttcagg gcatggttcg acgtagtcgc acgctggctt gcctcgttat
     1801 cgcagcaggg attgcagctt gcgagccgac gggccccgtc gacgccacga acacgcaaga
     1861 aggcgtcttg ccggcgacgg aaggcagcaa ggatttcggc gagtacgtgg tctacttcaa
     1921 cgccatcaaa accgatcagc tcgcgcccga catcgcgcgc gaatacggca tcgtgcgcag
     1981 caagagccgc gcgatgctga acgtcagcat tcatcacaag ctgccgaacg gaatgacgga
     2041 ggccgtcgaa ggcgccgtct cggcctcggc cgtcaacttg aacggccaac tgaagacgat
     2101 gacgctgcgc aaggtgcccg aggaaaacgc gatctactac atcggcgagc tcgggatcac
     2161 cgacggcgaa gtgctgatct acacgatcga cgtgacaccg cagggcgaag ccagccgctt
     2221 cacggtgcgc ttcaagaagc agtttttcgt cgaggagtga gacggccgcg cactctcaag
     2281 agcgcacgcg attgcggatg cggatcaggt ccgagtgcga cacgtagaga agctcggcga
     2341 ttctcctcac gagatgatcc tcgtgcttgt ccaaccggct gtccgccaac gccacctgcc
     2401 agagcatctc gacgacgcgc agcttctcgg cgaccgagag ctgctcgtgc aaacgacgcg
     2461 tgaattgctg tagctcgacg gcccggtcgg ccgtgcttcg agcctcttcg agcagcaaag
     2521 cggccgccgc atcgtcgagc gcgaagaact gcttcagcga ttggagcaag acctcgtcct
     2581 ccgccacctc ttcggcgtag tcggccttcg ccacctcgat cagcagcaga gcggtcgcga
     2641 tgcgcaacgc ctgctcgcgc gcggcggggt cgtccgcttt cggcgagaag gcgtcgtcga
     2701 agagcgcggc gagcttacga agcattggag cggccgtaag actcgagctc gggcccgaag
     2761 gtttcggtca cggcgcgcgt gatggcggcc aagctgccgt ggaagaaatg gccgacgccg
     2821 ggcaacagca cgatcttcgg cggcgacggc agcgccgagg cccattcgag cacgggtccc
     2881 ggcgggacga cgtcgtcttt gtcgccgtgg atgagcagcc acgggcagcg cggcgcggcg
     2941 aagccggcgc gcaaccgctc gaccggcggc gcgacggtga cgaggccggc cggttccaca
     3001 cgcgccgcga tcgacgccgc gaccgccgct ccgaacgaga aaccgccgag atagagcggc
     3061 cgagcaggcc aacgctcgcg gcaccaagcc acgaccgcga gcgcgtcgtc gccctcgccg
     3121 gttccgcccg catacgcgcc ggccgactcg ccgacgccgc gaaagttgaa gcgcacggcg
     3181 tccgcattga ggcgcgcgaa tgcgcgcgcg agggtcgtca cgaccttgtt cgtcatcgtg
     3241 ccttgctgct gcgggtgcgg gtgacagacg accgcggtcg cgacggccgg cccgacgctc
     3301 gcgtcgatcg ccgcttcgag cacgccggcg ggtcccggga tctcgatgct cgccgatcgc
     3361 ggcgctcgcg cggtcgactg gctcatccgc ggccgctctg cgcgccgagg tccaccaata
     3421 gctcgttcaa gcgcgcgacg aacgccgcgg ggtcctcgag ctgccgccct tcggcgagca
     3481 cagcttgctc gaacagcagg aacgcgagcc gttcgaagcg ccccgggtcg gactcggcgt
     3541 cgagccgctt gacgagcgcg tgctcgggat tcagctcgag gctcgggatc gccgccggcg
     3601 ctttgtggcc ggccgcggag agcagctcgc gcatctgata gccgagatcg tgctcgttca
     3661 gcacgaggca cgcggcggac tgccgcagcc ggccgccgac gcgcacctcg tcgattcgtt
     3721 cgcgaagcac ctgcttcacg cgcttcagca gcgcctgctg ctcgcggtct tgcggcttcg
     3781 ccttcggcgc cccgtccttg gcggcatcga agtcgagaac gccgcggcgc acgtccttga
     3841 aaggcttgcc gccgtgctgc gtgagatgct gcatgaccca ttcgtcgagc cggtcggtca
     3901 gaagcaatac ctcgatgccg cgctccttga acgcctcgag atgggggctc gttcgcgccg
     3961 ccgtcgccga ctcggcgacg aggaagtaga tcgcctcctg gtccggcttc gcatcgctca
     4021 agtactgctc gaggctccgg ctctgctcgt cgctcgccgt cttggtcgtg ccgaagcgca
     4081 tgagcttcgc gatccgctcg cgattcgcga cgtcttcgat gagcccttcc ttcagcaacg
     4141 cgccgaactg gctccagaac ttcgcgtatt tcgtcgagtc ctcggcgagc cgctcgagca
     4201 tgtcgagcac acggcgcgtc agcgcagcgc gcatcgcgcc gatcgcttca tcttgttgca
     4261 gcagctcgcg cgagacgttg agcgcgagct cgctcgaatc gacgacgccg cgaatgaagc
     4321 gcaggtacag aggcaaaaac tgcgtggcct gatcggtgat gaacacgcgc tgcacgtaca
     4381 gcttgacgcc cctcggcgct tcgcggttcc acaggtcgaa cggggccgtc gcgggcacgt
     4441 acaagagact cgtgtactcg cgcttgccct cgactcgatt gtggctccag atcagcggct
     4501 cttcgctgtc gtgcgcaagt gccatgtaga actcgacgta ctcgtcgtcc ttgatctcgg
     4561 cgcgcggccg cgcccagagt gccttcgcac gattgaccgt ctcctcctcg tggccgccgc
     4621 cggtcaagcg gaccggaaag gcgatgtggt ccgagtattt gcggatgagc gccttcagcc
     4681 gctccggctc gaggaattct gcggcgtttt ctttgagctt gagctgcacg gtcgtgccgc
     4741 gcgcaggccg ctcgacggtc tcgatgcggt actcgccgcg cccgtccgaa atccacgaga
     4801 cgccttcctc gggcggcgca tccgcgcggc gcgagagcac cgtcacctcg tccgccacga
     4861 tgaacgcgga gtagaacccg acgccgaact gcccgatcag ctgcgcatcc ttgcgctggt
     4921 cgccggtcag catctcgagg aactgcccgg tgccggagcg agcgatcgtg ccgagattgt
     4981 cgatgacctc gtcgcggctc atgccgatgc cggagtcggt gacgctgagc gtgcgctgct
     5041 cgggatcgac cgtgacgagg atgccgagct cgggctcggc cgcgctcagc tccggccgcg
     5101 cgaggctttc gaagcgcagc cggtcgttcg cgtccgaagc gttggagatc agctcgcgca
     5161 ggaaaatctc tcgctcgcta tacaacgagt gcaccatgag gtgcaggagc tgcttgacct
     5221 cggccttgaa tgcgaaagtg gacggctcgc cgctaaccat cggcccctcc ggatgctatc
     5281 gggatagggc taggttaggc gccgaccggg gtttttcaag cagtcgtcag gaaccgccgc
     5341 gcttctcggt ggtagacgac gcgcagcagc gtgcccgcga gccaggctac gacgctggcc
     5401 gccgcgacgt accagagtgc cggcgcccag gctgtcgcga tcgcggcgag ctgcgtcgcg
     5461 gcgaagccaa tcgccgatga gagcccgacc tgcaggacga ggcggcgcag gcgctcccag
     5521 gcgagcgcga cggaaaacaa ggcgtcgtcg agatcgtcca gccagagcag cacgacttcc
     5581 atgacgagcg ggcccagcgc tttaccggcg gctgcagtcg attttcacga gcgtcaatcc
     5641 gcgccgcggc cgagccggcg ggtcgcggtg ctcgtctttt ttgtgccgac gcgcggcatc
     5701 gatagccgaa acgatccatt ccaggctaca gatgctattc ttgtcgggcg agtgatcggc
     5761 gtgacctttt gcgtccattg ctcgaagatc tgcgctgtga cgaaagaact ataacgagcg
     5821 gcacgcgagc tctctgtgaa agtgctctca agaaccgaag cgacggcacg aagcctttta
     5881 cttattgcac gtacgcttcc tcactccgag tagactcttc cgagctgaag cgaatcatgc
     5941 aacggatcgt cgtcctcaat ccgaaaggtg gctccggcaa gaccacgatc gcgatcaacc
     6001 tggcggcctg ctacgcggcg aacggcggcc ggccggcact gatggatctc gatccgcagg
     6061 cctcctgcac gcgctggctg cgcaagcgtc ccgacgaggc ggccccgatc cacggcattg
     6121 cggcgttcga acggtcgccg agcgtcacgc ggagctggca gacgcgcgtt ccggcggact
     6181 gcggcgtcgt cgtcatcgac acgccggccg cgatcgatcc ccttgcgctg cccgatctca
     6241 cgcgcggggc cgatgccgtc ctcgtgccgg tcatgccctc ggacatcgac attcatgcga
     6301 ccgcgaagtg cattgcagac ttactgctcg tcgccaagat ccggcgctcg caaaagcgta
     6361 tcggcatcat cgccaaccgc gtgcgcggca atacgcgcgt ttcgcagtcg ctgacgcgct
     6421 tcctgaacag cctcgacatt ccgttgatcg ccacgctgcg cgacacacag agctacgtgc
     6481 gctcggcgga gacgggtcta ggggtttgcg agatgccgcg ctggcaggtc gggcaggacc
     6541 tgacgcattg gcgggagatg cttgcgtggc ttacggcgcg tcccggcagc cagcccgccg
     6601 cggggtcgcc taccaatctg ctatcgctcg agcggccgag cccgcagccg gaccgctcgc
     6661 cgctcgcgtc ggccgccggc cgcgcgggcg cggcgcgcga ctagggactt cggctcagcc
     6721 gtctttccac gactggcgga ccttgtccgc ccacgtgtgg tacccacgcc agctgtagat
     6781 cgaatgatcg agcggcgtac gctcgccggc cgatttcgtg ctgacgcgcg acaaccactt
     6841 tcgatacgcg tcccaaccgt gctggtcgtt cgagcgaggc tcgtcggctc cgttttgttc
     6901 tttggaatac aaggcgggtc ggaactgatg tggtttttgg tcgctcatgt ctgctgacaa
     6961 tccctgtccc cagatgagcg aagcctacgg cgccggcccc cgaaagccga ggaaccaagt
     7021 cacaaccgca gtcgtttggg cgcgccgctt ttgacgataa tgacggcaat cgtcaggcga
     7081 cgattcggta gcacggcacg taggcgctcg ggtcggtcaa gcgcatgcgc cgttcggtca
     7141 cgaagccctg cagcagggtg ccgagcgcgc cgatgatctt cgggtccccg cgcagctcga
     7201 acggcccgta tttctcgacg agcgcgatgc cggcctcgcg cacgttgccc gcgactatcc
     7261 ccgagaacgc tcggcgcaga ttgactgcca agctgtgcgc gggctgatcg cggtgcaact
     7321 cgagcgcgcg catcgattcg tgcgtcgcct cgaacggctt ctgaaactcg agaccgatct
     7381 cgagtgccca gttgaagaaa tacgcgttgt cgtccgcggc gcgccggtcg cgaacgcgcc
     7441 tgagcccgct cgtgacttcg gccgcgacgg ccggcgggtc gcccaggatg accttgtacc
     7501 gctgctgggc cacggggccc agcgtcgcgc cgatgaagct gtgtagccgc tcgaagtagc
     7561 tcttgctcga aggggggccc gtgaaaatca cgggcagagt ctcgccggag ttcttcgggt
     7621 cgagcaggat gccgagcaaa tagagaatct cctcggccgt gcccacgccg cccgggaaca
     7681 cgatgatggc gtgggccatc cgcacgaacg cctcgagccg cttctccata tcaggcagga
     7741 tcacgagctc gttgcagatc ggattcggcg attcggccgc gatgatcgtg ggctccgtga
     7801 gcccgaggta gcgcccgttg cgaatacgct gcttcgcgtg cccgacggtc gctcctttca
     7861 tggggccttt cattgcaccg gcgccgcagc ccgtgcagat gtcgaggccc tggagtccga
     7921 gcgcgtagcc gacgaccttc gtgtagtcgt actcctcgcg ctcgatcgcg tggccgcccc
     7981 agcacacgac gaggcgtggg tcctcgtgcg ctttgatcac gcgcgcgttg cgcaggatgt
     8041 gaaacacggc atcggtgagg ccgtcggtgg tcttgaggtc gaacgtggga ttcttgagca
     8101 cgttctcgtg cgcgtagagc aagtcgcgga gc
//
LOCUS       DASSNN010000294         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1439270, whole genome shotgun sequence.
ACCESSION   DASSNN010000294 DASSNN010000000
VERSION     DASSNN010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439270"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFL84_05380"
     CDS             complement(<1..676)
                     /locus_tag="VFL84_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7608087.1"
                     /translation="MPRAPRLHVPGGLYHAVLRGNHQHVIFDGGADYRTFEDILARAL
                     HRYGARLHAYCWMTNHVHLAVQVGDVRLGRLMQAVASSYARRKQQQVPTTGHLFERRY
                     RAILVDNDAYWLTVIRYIHRNPVEAGIVADPADYRWSSHRGYLGRGRPPWLTTAPTLA
                     MFGCGAVDSVAAYRRFVAGDTAVEAAPSSATVVTPHSLIGFELDKRGNVQSLEQVVLE
                     VSSELSV"
     gene            complement(844..1680)
                     /locus_tag="VFL84_05385"
     CDS             complement(844..1680)
                     /locus_tag="VFL84_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HET7608088.1"
                     /translation="MRMSRSDFLAWPHKAITLLGMSGVGKTTLAYKLPASKWFHYSGD
                     YRIGTKYLAEPILDNIKRQAMQVDFLRDLLRNDSIYIASNITFHNLQPVSTFLGKIGR
                     TDMGGLPVAEFKRRQRLHRDAEISAMRDVAEFIVKAQEIYGYDHFINDAGGSLVELDD
                     EKTEQTLAENTLILYLKADEEMERELVRRAVSDPKPLYYNEKFLDQKLAEYVAEAELQ
                     SPDDIVPDEFVRWIFPSLVAHRRPKYEALAKRHGYTLNARLAEAITNERDFLELVAHA
                     LD"
     gene            complement(1748..2443)
                     /locus_tag="VFL84_05390"
     CDS             complement(1748..2443)
                     /locus_tag="VFL84_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HET7608089.1"
                     /translation="MISTRRQFIRGATLSAAALWAPGAFAQALATAVTGDGPYYPDRL
                     PLDTDNDLLILNDRLTPAVGEIAHFGGRLFAENGAPVRNAFIEIWQADVNGSYIHTQG
                     RNDGKLDANFQGYGRFLTDAEGRYYFRTIKPVPYTLQGQFRAPHIHVAVSKAGKRIMA
                     TQAMVRGHAANKGDLIVERIKDPKLLETIMVEYEPLPGSALGELTATFDIRLGRTAVE
                     LGDGTIGGIGKPG"
BASE COUNT          455 a          818 c          862 g          472 t
ORIGIN      
        1 caacgctaag ctcgctcgag acctcgagca cgacctgctc gagcgattga acgttcccgc
       61 gtttatcgag ctcgaagccg atcaacgaat gtggcgtgac aaccgtcgcg ctagatggag
      121 ctgcctcgac cgcggtatcg ccggcgacga agcgcctgta ggcggcaacc gaatcgacgg
      181 cgccgcagcc aaacatcgcc agagtcggcg ccgtcgtgag ccaaggtggg cggccgcgac
      241 cgaggtaacc gcgatgactg ctccaccgat aatccgcggg atccgcgacg atgccagcct
      301 cgacggggtt gcggtgaatg taccgaatga ctgtcaacca atacgcatcg ttgtcgacga
      361 gaatggcgcg gtagcggcgc tcgaataagt gccctgttgt cggcacttgt tgctgtttgc
      421 gtcgcgcgta gctcgacgca accgcctgca tcagacggcc gagccgcacg tcaccgacct
      481 gcaccgccag atggacatga ttcgtcatcc agcagtaggc gtgcaagcgt gcgccgtagc
      541 ggtgtagcgc tcgcgccagg atatcttcga aagttcgata gtccgcacca ccatcgaaaa
      601 tcacgtgttg gtggttaccg cggaggacgg cgtgatacag gccgccggga acgtgcagac
      661 gtggggcgcg aggcatgggc acctagcaga gggacgaggt gatgccatga tgtcgagctt
      721 ggacaccgct gggcctctcg cgaaatcgtg gattgtgccg agattggaga gccagggggc
      781 cgacgttcgg ttcaaccgaa ccgcaacacc ggcaccacgc ctagtccaac accggcacca
      841 cgcctagtcg agggcgtggg cgacgagctc gaggaaatcg cgctcgttcg tgatcgcttc
      901 ggctaggcgc gcgttcagcg tgtagccgtg gcgcttcgcg agcgcctcgt acttcgggcg
      961 gcggtgcgcg acgagtgacg gaaagatcca gcgcacgaac tcgtcgggca cgatatcgtc
     1021 ggggctctga agctcggctt ccgcgacgta ctcggcgagc ttctggtcga gaaacttctc
     1081 gttgtaatag agcggcttcg gatcgctgac ggcgcggcgc acgagctcgc gctccatctc
     1141 ctcgtccgcc ttcaagtaca ggatcagcgt gttctcggcc aacgtctgct cggtcttctc
     1201 gtcgtcgagc tcgaccaggc tgccgcccgc gtcgttgatg aaatgatcgt agccgtagat
     1261 ctcctgcgcc ttgacgatga actcggcgac gtcgcgcatc gcgctgatct ccgcgtcgcg
     1321 gtgcagccgc tgccgccgct tgaattccgc gacgggcagc ccgcccatgt cggtacggcc
     1381 gatcttgccg aggaacgtcg agacgggctg cagattgtga aacgtgatgt tgctcgcgat
     1441 atagatcgag tcgttgcgca gcaaatcgcg caggaagtcg acctgcatcg cttgccgctt
     1501 gatgttgtcg aggatcggct cggcgagata cttcgtgccg atccgatagt cgccggagta
     1561 atgaaaccac ttcgacgccg gcagcttgta ggcgagcgtc gtcttgccga ctcccgacat
     1621 gccgagcagc gtgatggcct tgtgcggcca ggccaggaaa tccgagcggc tcattcgcat
     1681 ggcaagggat ccgaagactt ggcgggcgga ttatacggtg cccgcaggcc cgaacgcgct
     1741 cgcgccgtca gcccggtttg ccgatgccgc cgatcgtgcc gtcgccgagc tcaacggccg
     1801 tccggccgag cctgatgtcg aacgtcgccg tgagctcgcc gagcgccgag cccggcagcg
     1861 gctcgtactc gaccatgatc gtctcgagca acttgggatc cttgatgcgc tcgacgatca
     1921 agtcgccctt gttcgccgca tgaccgcgca ccatcgcctg cgtcgccatg atgcgcttgc
     1981 cggccttgct gacggcgaca tgaatgtgcg gcgcgcgaaa ctggccctgc agcgtgtacg
     2041 gcacgggctt gatcgtgcgg aagtagtagc ggccttcggc atccgtcagg aaccggccgt
     2101 agccctggaa gttcgcgtcg agcttgccgt cgttgcgccc ttgcgtgtgg atgtaggagc
     2161 cgttcacgtc ggcctgccag atctcgatga acgcgttgcg cacgggcgcg ccgttctcgg
     2221 cgaagagccg cccgccgaag tgcgcgatct cgccgacggc cggcgtgagc cggtcgttca
     2281 aaatcaagag gtcgttgtcg gtgtcgagcg gtaagcggtc gggatagtac ggcccgtcgc
     2341 ccgtgacggc cgtggccagc gcctgcgcga acgcgccggg cgcccagagt gccgcggcgc
     2401 tcaaggtcgc gccgcgaatg aactgccgtc gcgtcgagat catgggcttg ccccctcgtc
     2461 tggcgccgta tcttatgcgc agccgcttga gcctacgccc gcggccgagc ccgggcaacg
     2521 cggtggcgca tctgattcgt tccgggccgc atcttagggg gtcatgaaag cgtgcacccg
     2581 cgcacggctt aggaaaccga ggtaatt
//
LOCUS       DASSNN010000295         3562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_796921, whole genome shotgun sequence.
ACCESSION   DASSNN010000295 DASSNN010000000
VERSION     DASSNN010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796921"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFL84_05395"
     CDS             <1..747
                     /locus_tag="VFL84_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608090.1"
                     /translation="TLLLFPFALLVPAMLMPGKTRQLRKVAPVIAVGFVAVTAFVIIY
                     NYLIQYREYAGPISEYFTWDTLRYYLYTGAATTDEAYIGRFDSIEIAFDHISQDPLKL
                     AFGYGAGNVSESFLPQFAGRYWSYFMRFGVGETQITTFLWEIGFVGFAVYLYLFAIVA
                     RDAVTLARSNDDAAPLGQIWAVVVIVMTFALVYKAVFAMNDFGYLFWYFSGVVASRAV
                     AVRQAARKRFAQAPESWRLAAGDSRAAVKG"
     gene            981..1784
                     /locus_tag="VFL84_05400"
     CDS             981..1784
                     /locus_tag="VFL84_05400"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7608091.1"
                     /translation="MQHERAQGETRNGTGGALATAYAKPSAAAPRQRAARRSSVRDWL
                     RGIAFGLSERFRAKRAERFRREFDLGEETRVLDLGGGDGEHIRMVLECTPVRPENVYV
                     ADVNAKALERAKAYGHRTTLLKQDGRVPFPDGFFDVVFCSSVIEHVTVPKSEVWQHRN
                     ELFARKAVAHQRAFADEISRIAKSFWVQTPARSFFLETHSWLPFFGWLPRAAQVAILS
                     VTRHFWLSRHPPDYYLLTRGHMEALFPGARIVSERWLGMTKSWIALRRH"
     gene            1876..3102
                     /locus_tag="VFL84_05405"
     CDS             1876..3102
                     /locus_tag="VFL84_05405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014530188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7608092.1"
                     /translation="MTATLLSINNYYYPRGGAEVVFFRHNGMLHDAGWDVVPFAMNHR
                     MNVGGERSEFVNEIEYGRETDGVVTKIRNGLKAVYSFEARTKLARLIDRAGPDLCHAH
                     NVYHHLSPSVLGLVRKRGIPLVMTLHDLKIACPAYSMLTHDGICERCKDGRLYQVVTH
                     RCMKGSLALSALVMLESYLHLLLGSYMRNVDRFLVPSRFYLRKLGEWGFPAERFEYVP
                     NFVAAESFEACYEPGARFVYFGRLSREKGVATLIRAAAEARVGIDVIGTGPAERELRA
                     LAADRDVRFLGYLTGTKLNAAVCSARAVVVPSEWYENAPLAVLEGAALGKPLVVSRIG
                     GLQELVVDGESGWSFEAGSVDELAAVLRRVAALPDAAVAAAGMAARRRIEDEFSPARY
                     LERIRAVYGRLGVSWP"
     gene            3099..>3562
                     /locus_tag="VFL84_05410"
     CDS             3099..>3562
                     /locus_tag="VFL84_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7608093.1"
                     /translation="MSAPAPDVQARMRIMVLGLRGIIDVQGGIETHARRLYPLLARLG
                     CDVEIVQRSPYYPRQGRRREWRGIALTYIWSPKTRVVETAVHTLLGVLYAAIKRPDVL
                     HLHAVGPGLLAPLARLFGLRVVVTHHGADYEREKWGPLAKAVLRTGERLGVG"
BASE COUNT          561 a         1198 c         1167 g          636 t
ORIGIN      
        1 acgttgctgc tgttcccgtt cgcgttgctg gtgcccgcga tgctgatgcc gggcaagacg
       61 cggcagttgc gcaaggtcgc tcccgtaata gcagtcgggt tcgtggccgt gacggcgttc
      121 gtgatcatct acaactatct gatccagtat cgcgaatacg cgggtcccat cagcgaatac
      181 ttcacgtggg acacgctgcg gtactacctc tacacgggtg ccgcgacgac cgacgaggcc
      241 tacatcggcc gcttcgacag catcgagatc gcgttcgacc acatcagcca agatcccctc
      301 aagctcgcct tcggttacgg agccggcaac gtttcggagt cgttcctgcc ccagttcgcg
      361 ggcaggtact ggtcgtactt catgcgcttc ggcgtcggcg aaacgcagat cacgactttt
      421 ctctgggaga tcggtttcgt cggcttcgcc gtctatctgt atctgttcgc aatcgtcgcg
      481 agggacgccg tcacgctcgc gcgctcgaac gacgacgcgg cgccgctcgg ccagatctgg
      541 gccgtcgtag tcatcgtcat gacgttcgcg ctcgtctaca aggccgtgtt cgcgatgaac
      601 gacttcggct acctgttctg gtatttctca ggtgtagtcg caagccgcgc agttgccgtg
      661 cgacaggcgg cacgaaaacg cttcgcccaa gcccccgaga gctggcggct cgcggccggc
      721 gactcgcgcg cagccgtcaa gggttgacgc gcaaaggatt gcgccggcgc ggcgcgctcc
      781 acgatcatcg cgcgctcgca cctcgagcac ggccgccgcg caacagggtc cttggcacgg
      841 attgtgctgt tctttccccg ttagcggatg tggctagggg tggccgtggc caaaaaacca
      901 aagcgcaagt tctgcgagca agctcgagaa aacctgcggc acgcgctgca ggcacgcgag
      961 cgcgcggcgt agccgtggcc atgcagcacg agcgagcaca gggcgaaacg cgcaacggaa
     1021 cgggcggcgc cctcgcaacc gcctacgcga aaccgagcgc cgccgcgccg cgacagcgcg
     1081 ccgcgcgaag atcttccgtc agggattggc ttcgcggcat cgcgttcggg ctttccgagc
     1141 gctttcgcgc gaaacgcgcc gagcgcttcc gccgcgagtt cgacctcggc gaggagacgc
     1201 gcgtgctcga cttgggcggc ggcgacggcg agcacattcg catggtgctc gaatgcacgc
     1261 cggttcggcc cgagaacgtc tatgtcgccg acgtcaacgc caaggcgctg gaacgcgcga
     1321 aagcctacgg ccatcgcacg acgttgctca agcaggacgg gcgcgtgccg ttccccgacg
     1381 gcttcttcga cgtcgtgttc tgctcgtccg tcatcgagca cgtgaccgtg ccgaaaagcg
     1441 aggtctggca gcaccgcaac gagctcttcg cgcgcaaggc cgtcgcgcac cagcgcgcgt
     1501 tcgcagacga gatttcgcga atcgcgaaat cgttctgggt gcaaacgccg gcacgatctt
     1561 ttttcctcga aacgcattcc tggctgcctt tcttcggctg gctgcctcgc gccgctcagg
     1621 tcgcgattct gagcgtcacg cgccatttct ggctgtcgcg gcatccgccc gactactacc
     1681 tgctgactcg cggacacatg gaggcgctgt tccccggcgc gcgaatcgtc agcgagcgct
     1741 ggctcggcat gacgaaatcc tggatcgcgt tgcggcgaca ttaacggacg gcaaagcaat
     1801 ggattgctcc cacaactcgt tcccgcgctt tcgacgactc gccgcgacgc gcaaggccgt
     1861 cgaggatcgc cgtcgatgac cgcgacgttg ctcagcatca acaactacta ctacccgcgc
     1921 ggcggcgccg aggtcgtgtt cttccggcac aacggcatgc tgcacgacgc gggttgggac
     1981 gtcgtgccgt tcgcgatgaa tcatcgaatg aacgtcggcg gcgaacgatc cgagttcgtg
     2041 aacgaaatcg agtacggccg cgagacggac ggcgtcgtga ccaagattcg taacgggctg
     2101 aaagccgtct attcgttcga agcgcgcacc aagctcgcgc gcctcatcga tcgtgccggc
     2161 ccggatcttt gccacgcgca caacgtctat caccatttgt cgccgtcagt cttgggtctc
     2221 gtgcgcaagc gcggcatccc gctcgtgatg acgctgcacg atctcaagat cgcgtgcccc
     2281 gcgtattcga tgctgactca cgacggcatc tgcgagcgct gcaaggacgg ccgcctctac
     2341 caggtcgtga cgcatcgctg catgaagggc agcctcgcgc tgtcggcgct cgtcatgctc
     2401 gagagctacc tgcacctgct gctcggcagc tacatgcgca acgtcgatcg cttcctggtg
     2461 ccgagccggt tctatttgcg caagctcggc gagtgggggt ttccggccga gcgcttcgag
     2521 tacgtgccga atttcgtcgc ggcggaatcg ttcgaggcct gctacgagcc cggcgcgcgg
     2581 ttcgtctact tcggccgcct gtcgcgagaa aaaggcgtcg ccacgttgat ccgcgcggcg
     2641 gccgaggcgc gcgtcgggat cgacgtcatc ggcacggggc cggccgagcg cgagctgcgc
     2701 gcgctcgcgg ccgaccgcga cgtgcgtttc ctcgggtacc tcaccggcac gaagctgaac
     2761 gccgccgtat gctcggcgcg cgcggtcgtc gtgccctccg aatggtacga gaacgcgccg
     2821 ctcgccgtgc tcgagggcgc tgctctcggc aagccgctcg tagtctcgcg catcggcggt
     2881 ctgcaggagc tcgtcgtcga cggcgagtcg ggttggagct tcgaggcggg ctccgtcgac
     2941 gagctggccg ccgtgctgcg gcgtgtcgcc gcgcttcccg atgccgccgt cgcggccgcg
     3001 ggcatggccg cacggcgccg catcgaggac gaattctcgc cggcgcgcta tctcgagcgc
     3061 attcgcgccg tctacggcag gctcggcgtc tcgtggccat gagcgcgccc gctcccgacg
     3121 tgcaagcgcg catgcgcatc atggtgctcg ggctgcgcgg catcatcgat gtgcagggcg
     3181 gcatcgagac gcacgcgcgg cggctctatc cgctcctggc gcgcctcggc tgcgacgtcg
     3241 agatcgtgca gcgcagcccc tactacccgc gccagggccg tcgccgagaa tggcgcggca
     3301 tcgcgttgac ctacatctgg agccccaaga cgcgcgtcgt cgagactgcg gtgcacacgt
     3361 tgctcggcgt gctctacgcg gccatcaagc ggcccgacgt gctgcatctg cacgccgtgg
     3421 gaccggggtt gctcgccccg ctcgcgcggc tcttcgggct gcgcgtcgtc gtcacgcatc
     3481 atggcgccga ctacgagcgc gagaagtggg ggccgctcgc gaaggcggtg ttgcgcaccg
     3541 gcgagcgcct cggcgtcgga tt
//
LOCUS       DASSNN010000296         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_797419, whole genome shotgun sequence.
ACCESSION   DASSNN010000296 DASSNN010000000
VERSION     DASSNN010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797419"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1099
                     /locus_tag="VFL84_05415"
     CDS             161..1099
                     /locus_tag="VFL84_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7608094.1"
                     /translation="MSKLRTAVVGVGYLGRFHAQKHRALDNVELVAVCDRDLERGRAV
                     AAEVQTSAVQDYRDLVGKIDAVTIAATTAEHFPIAKFFLEHGVHCLVEKPLTRTSAEA
                     SVLTELAAQRKVKLQVGHVERFNPALLAARETLTTVRFIECHRLAPFKGRGADVNVIL
                     DVMIHDLDVILSLVDSKPRVVSAVGIPVLMDDIDIANARIEFEDGAIANVTASRVSTT
                     PQRRFRVFQPNQYVSIDFGNGEVRRVTKLGAWREGESPLREETWNLEKGDALLAETKA
                     FVDAIENDTPPVVSGADALASLELAELINADIARRR"
     gene            complement(1196..2599)
                     /gene="ntrC"
                     /locus_tag="VFL84_05420"
     CDS             complement(1196..2599)
                     /gene="ntrC"
                     /locus_tag="VFL84_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HET7608095.1"
                     /translation="MATPGLSVWIVDDDESVRWVLEQALKQAHMFPRAFEAGEQFFDA
                     LKLGRPDVLITDIRMPDMSGLELMERLARTDPDIPVIVITAHSDLDSAVSAYQGGAFE
                     YLPKPFDIDEAIELVSRAARQRTRGSTEPTTVKPTPRIIGQAPAMQEVFKAIGRLSRS
                     SMTVLITGESGTGKELVARALHDNSPRANGPFIALNTSAIAAELLESELFGHEKGAFT
                     GASERRIGRFEQANGGTLFLDEIGDMSLALQTRLLRVLAESEFFRVGGQTPIRVDVRV
                     IAATHQDLGQGAATGRFREDLYHRLNVMRIEVPPLRQRREDIRELSRFYLEQAANELG
                     VAPKTLSADALEALQRYDCPGNVRELVNVCRRLTVTAAGREIVAQDLPAELGGVASQS
                     GADWTAGLTQWAEAQLAGVEPPPLLSDALPAFERTLILTALRRARGKRLEAAKLLGWG
                     RNTLTRKIKELGLEGRV"
BASE COUNT          421 a          908 c          883 g          435 t
ORIGIN      
        1 gacgaacgcc gcaacctcga cacggcgctg cgtttcgtgc tcgatcgcgc gttgacgatc
       61 gaggaggtcg ccggccgcat cgcagccgaa tgcgccgcga gcccgcagat cgcgcacttg
      121 ctcgcgttca tcggcagctc cgagcgcggg atcgcacgcg gtgagtaagc tgcgcacggc
      181 ggtcgtcggc gtcggctacc tcggccgttt tcacgcgcaa aagcaccgcg cgctcgacaa
      241 cgtcgagctc gtcgccgtct gcgaccgcga cttggagcgc ggccgcgccg ttgcggccga
      301 ggtgcaaacg agcgccgtgc aggactatcg cgacctcgtc ggcaagatcg acgccgtcac
      361 gatcgcggcg acgacggccg agcattttcc catcgcgaaa ttctttctcg agcacggcgt
      421 gcactgcctc gtcgagaagc cgttgacgcg cacgagcgcg gaggcatccg tgctcacgga
      481 gctcgccgcg cagcgcaagg tgaagctgca ggtcggccac gtcgagcgat tcaatccggc
      541 gctgctcgcg gcgcgcgaga cgctgacgac cgtgcgtttc atcgagtgcc atcggctcgc
      601 gccgttcaag ggccgcggcg ccgacgtcaa cgtgattctc gacgtcatga ttcacgacct
      661 cgacgtgatt ctgtcgctgg tcgactcgaa gccgagagtc gtgtcggccg tcggcattcc
      721 cgtgctcatg gacgacatcg acattgccaa cgcgcgcatc gagttcgagg acggcgcgat
      781 cgcgaacgtg accgcgtcgc gcgtctcgac gacgccgcag cgccgcttcc gcgtgttcca
      841 gccgaatcag tacgtttcga tcgacttcgg caacggcgag gtccggcgcg tcacgaagct
      901 tggggcttgg cgggaaggcg agtcgccgct gcgtgaggaa acgtggaatc tcgagaaggg
      961 cgacgcgctg ctcgccgaaa ccaaagcctt cgtcgacgcg atcgagaacg acacgccgcc
     1021 agtcgtctcc ggcgccgacg cgctcgcatc gctcgagctc gccgagctga tcaacgctga
     1081 catcgcccgc cgccgctaaa aggggacaga tctattttcc gcgcgtcgat ggattgcccg
     1141 cgactaacgt agtcgcgcgc ggaaaataga tctgtcccct tttagcgtcc tcgttctaga
     1201 cgcggccttc gaggccgagc tccttgatct tgcgcgtcag cgtgttgcga ccccagccga
     1261 gtagcttcgc ggcttcgagg cgcttgccgc gcgcgcggcg cagcgccgtc aggatcaacg
     1321 tgcgctcgaa tgccggcagc gcgtcgctca atagcggcgg cggctcgacg cccgcgagct
     1381 gcgcctcggc ccactgcgtc aagcccgcgg tccagtcggc gccggactgg ctcgccacac
     1441 ctccgagctc ggccggcaag tcctgcgcga cgatctcgcg gcccgcggcc gtgaccgtga
     1501 gccggcggca gacgttgacg agctcgcgga cgttgccggg acagtcgtaa cgctgcagcg
     1561 cttcgagcgc gtccgcgctc aacgtcttcg gcgcgacgcc gagctcgttg gccgcctgct
     1621 cgagatagaa ccgcgacagc tcgcggatgt cctcgcggcg ttgccgcagc ggcggcacct
     1681 cgatgcgcat gacgttcaag cggtgataga ggtcctcgcg gaagcgcccc gtcgcggcgc
     1741 cctgcccgag atcctgatgc gtcgcggcga tcacgcgcac gtcgacacgg atcggcgtct
     1801 ggccgccgac gcggaagaac tcgctctcgg cgagcacgcg cagcaagcgc gtctggagcg
     1861 cgagcgacat gtcgccgatc tcgtcgagga acagcgtgcc gccgttcgcc tgctcgaagc
     1921 ggccgatgcg ccgctcgctc gcgcccgtga acgcgccctt ctcgtggccg aacagctccg
     1981 actcgagcag ctcggccgcg attgcggagg tattcaacgc gatgaacggc ccgttcgcgc
     2041 gcggcgagtt gtcgtgcagc gcgcgcgcga cgagctcctt gcccgtgccc gactcgcccg
     2101 tgatcagcac cgtcatgctc gagcgcgaca gccggccgat cgccttgaag acttcctgca
     2161 tcgccggcgc ttggccgatg atgcgcggcg taggcttcac ggtcgttggc tcggtggagc
     2221 cgcgcgtgcg ctgccgcgcg gcgcgcgaga cgagctcgat cgcctcgtcg atgtcgaacg
     2281 gcttcggcaa gtattcgaac gcgccgccct gatacgcgga gaccgcactg tcgaggtccg
     2341 aatgcgccgt gatgacgatg accggaatgt cgggatccgt gcgcgcgagg cgctccatga
     2401 gctcgaggcc gctcatgtcc ggcatgcgga tgtcggtgat caagacgtcg gggcgcccga
     2461 gcttcagcgc gtcgaagaac tgctcgccgg cttcgaacgc gcgcggaaac atgtgggctt
     2521 gcttgagcgc ttgctcgagc acccaccgta ccgactcgtc gtcgtcgacg atccatacgg
     2581 acaaacctgg tgttgccatt agtggcggcc tccattcacc accggcagcc ggatctggaa
     2641 cacggtt
//
LOCUS       DASSNN010000297         2037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_560199, whole genome shotgun sequence.
ACCESSION   DASSNN010000297 DASSNN010000000
VERSION     DASSNN010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560199"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="VFL84_05425"
     CDS             complement(<1..129)
                     /locus_tag="VFL84_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011771932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7608096.1"
                     /translation="MSLQALRRYPLRTSMLLTAIAIGVAAVVLLTAVGEGARRYVTG"
     gene            complement(157..834)
                     /locus_tag="VFL84_05430"
     CDS             complement(157..834)
                     /locus_tag="VFL84_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008224554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7608097.1"
                     /translation="MMELSGICREYVVGAETVHALDHVDLTIEDGEYVSIMGPSGSGK
                     STLLNVLGLLDRPTSGTYRLQGEDVSSLDDNALAAARQRHIGFIFQFFHLIPRLTALE
                     NVELPLVLTGAPPRARRERAAAILASVGLEKRLDHRPDQLSGGERQRVAIGRAIVMQP
                     SYLLADEPTGNLDTRSGNEIIQILERLNRDGIALLIVTHDPAVGDRAQRHLKLRDGKI
                     VGDVRET"
     gene            complement(854..1981)
                     /locus_tag="VFL84_05435"
     CDS             complement(854..1981)
                     /locus_tag="VFL84_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7608098.1"
                     /translation="MKRIAIVAALVVAVLAAVWFFSRPEPVAVTVVTAERGPVAATVT
                     NTRAGTVDACRRAGLSPALGGQIASLPVKDGDRVEAGELLLELWNADVVAQLTLSERD
                     ARAARSRAREACIVADVAKRDAERLTKLRADRLASEEAADQAEGKAQSTAAACAAAED
                     ATRVAEARVDVTKAQLERTQLLAPFAGVIAEINGEVGEFVTPSPVGIPTPPTVDLIDA
                     SCLYVSAPIDEVDAPRVRAGLPARISLDAFPDRSFPGHVRRVAPYVLDQEKQARTVEI
                     EAEFDDAEEAGLLAGYSADVEVVLEERDDVLRLPTPVILPDKTVFVYDDATGTIASRA
                     VETGIANWEFTEILSGVEPGDRVVSSIDRDGVVDGAVVRIE"
BASE COUNT          305 a          708 c          715 g          309 t
ORIGIN      
        1 cccagtgaca tagcggcgcg cgccttcgcc gacagccgtc aagagcacga cggccgcgac
       61 gccgatcgcg atcgcggtga gcagcatcga ggtgcgcagc gggtaacgcc gcagcgcctg
      121 cagcgacatg ttcgtcacgt cgatgggctt cacgtttcag gtctcgcgca cgtcgccgac
      181 gatcttgccg tcgcgcagct tcaaatgacg ctgcgcgcgg tcgccgaccg ccgggtcgtg
      241 cgtgacgatc aagagcgcta tgccgtcgcg attcagccgc tcgaggattt ggatgatctc
      301 gttgccggag cgcgtgtcga gattacccgt cggctcgtcc gcgagaagat agctcggctg
      361 catgacgatc gctcgaccga tcgcgacgcg ttggcgctcg ccgcccgaga gctgatcggg
      421 ccgatggtcg agccgcttct cgagcccgac cgacgccagg atcgcggcgg cgcgctcgcg
      481 gcgcgcgcgc ggcggggcgc cggtcagcac gagcggcagc tcgacgttct cgagcgccgt
      541 gagccgcggg atcaggtgaa agaactggaa gatgaagccg atgtggcgtt gccgggcggc
      601 cgccagcgca ttgtcgtcga ggctcgacac gtcctcgcct tgcaggcgat aggtgcccga
      661 ggtcgggcgg tcgagcagcc cgagcacgtt cagcagcgtc gatttgccgg accccgaggg
      721 ccccatgatc gagacgtact ccccgtcctc gatggtcagg tcgacgtgat cgagcgcgtg
      781 caccgtctcg gcgccgacca cgtactctcg gcatattccg gagagctcca tcatgggcgg
      841 ccgctacgga acgctattcg atgcggacga cggcgccgtc gacgacgccg tcgcgatcga
      901 tcgagctcac gacgcggtcg cccggctcga cgccgctcag gatctcggtg aattcccagt
      961 tcgcgatgcc ggtctcgacg gcgcgcgacg cgatcgtgcc cgtcgcgtcg tcgtacacga
     1021 acacggtctt gtcgggcagg atcacggggg tcggcaggcg cagcacgtcg tcgcgctcct
     1081 cgagcacgac ctctacgtcg gcgctatagc cggcgagcag cccggcttcc tcggcgtcgt
     1141 cgaactcggc ctcgatctcg acggttcgcg cctgcttctc ctgatccagc acgtacggcg
     1201 cgacgcggcg cacgtgcccg ggaaaggaac ggtcggggaa cgcgtcgagg ctgatgcgcg
     1261 cgggcagccc ggcccgcacg cgcggcgcgt cgacctcgtc aatcggcgcg ctgacgtaga
     1321 ggcacgacgc gtcgatgaga tccacggtgg gcggcgtcgg tatcccgacc ggcgacggcg
     1381 tcacgaactc gccgacctcg ccgttgatct cggcgatgac gcccgcgaac ggcgcaagca
     1441 gctgcgtacg ctcgagctgc gccttcgtga cgtcgacgcg agcttccgca acgcgcgtcg
     1501 catcctcggc agccgcgcac gcggccgccg tcgattgcgc tttgccttcc gcttgatcgg
     1561 ccgcctcctc ggacgcgagc cggtccgcgc gcagcttcgt caagcgctcc gcatcgcgtt
     1621 tcgcgacgtc ggcgacgatg caggcctcac gggcgcgcga gcgcgccgcg cgcgcatcgc
     1681 gctccgatag cgtgagctgc gccacgacgt cggcgttcca gagctcgagc agcaattcgc
     1741 ccgcttcgac gcggtcgccg tccttgacgg gcaagctcgc gatctggccg ccgagcgcgg
     1801 gcgacagtcc cgcgcggcgg cacgcgtcca cggttccggc gcgcgtgttc gtgacggtgg
     1861 cggccacggg gccgcgttcg gccgtgacga cagtcaccgc cacgggctcg ggccgcgaaa
     1921 aaaaccagac cgcggccagc acggcgacga cgagcgccgc aacgatggcg atgcgcttca
     1981 tggccacttc gagtggagga tcggcgggtc agtctaacgg attggggcgg gcagcgc
//
LOCUS       DASSNN010000298         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_797725, whole genome shotgun sequence.
ACCESSION   DASSNN010000298 DASSNN010000000
VERSION     DASSNN010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797725"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..379)
                     /locus_tag="VFL84_05440"
     CDS             complement(164..379)
                     /locus_tag="VFL84_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608099.1"
                     /translation="MRSFLALVGWLLLFVLCWPVALLALVLWPLVWLISLPFRLLGIA
                     FDAVFAFLRAVLFLPARVLGHRDSGRA"
     gene            407..1300
                     /locus_tag="VFL84_05445"
                     /pseudo
     CDS             407..1300
                     /locus_tag="VFL84_05445"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Acylates the intermediate (KDO)2-lipid IVA to form
                     (KDO)2-(lauroyl)-lipid IVA; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
     gene            complement(1326..1970)
                     /locus_tag="VFL84_05450"
     CDS             complement(1326..1970)
                     /locus_tag="VFL84_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-associated protein"
                     /protein_id="HET7608100.1"
                     /translation="MTLSFAAFAETGNAQPAEAIDVALRTLFAILVAVVVPVYLVKYG
                     PTNFLWFSDIGLLGICAALWLEHGLLGSMMALAVLIPETLWLVSFVTGAIRKGLGVTT
                     LATYMFDASIPLSLRALSAAFHLALLPGALWLVYRYGYDSRAFVAQTLLAWVVLPATL
                     WLAPPEKNVNWVRGFGHPPRRTLPLPVHFCIVMLAYPLLVYLPAHFLLGRLFGG"
     gene            complement(2002..>2633)
                     /locus_tag="VFL84_05455"
     CDS             complement(2002..>2633)
                     /locus_tag="VFL84_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608101.1"
                     /translation="KMSIDLSRAAVPTPGQHDPLDGYVTYVQLQATAELAPPEAHQPE
                     VRELADDLVATIEASDLATADPLGLGGLLMDAARIEQLTDAGAFRNETLATFVLASAA
                     NGLDYYERAGGPRQPVRLRLPFRELGLALGLHGLELIAAKAASRRLQATTRVLERYTP
                     MVAEIERFWLEPSSQETASWTAHRDINDVTLAAALTPAGMIELPRPPVG"
BASE COUNT          486 a          871 c          894 g          382 t
ORIGIN      
        1 tacgaacgat cggctcgtcg ccgagttctt cgccgtcaag catggctaca tccaggaaat
       61 tcaggccgtg ctgttcaacc tgcccgatgc gcaagcgacg ggctggccgc cggacacggg
      121 gcccggccgc gggtccgcga aatagcgtgg cttggtcgcg cgcctacgct cgtccagagt
      181 cgcgatgacc aagcacgcgc gccggcagga acaagacggc acgcaagaac gcgaacacgg
      241 cgtcgaacgc gatgccgagc agccggaacg gcagcgagat cagccagacg agcggccaca
      301 gcacgagcgc gagcagcgcg accggccagc acagcacgaa cagcagcagc caccccacga
      361 gggcgagaaa ggatcgcatg gcgccaccgt agcgcggttc ttaggtccgc gctactggcc
      421 tgcgtgggcg ctgtggggct ggatgcggct cgcggcggcg ttgccgatca aggtcgcgtt
      481 ggcgattcat cggccgatcg gcaaggtcat gtaccggctc ctcggccggc agcgcaagac
      541 ggtgctgcgc aatctcgagc tgtgctttcc cgagctcgcg ccgcacgagc gcgaggcatt
      601 ggccaggcgc cacttcgaag ccgtcggcgt ttacttcgcg gaatccgcga tcgcgtggtt
      661 cggctccgag cgtcacgtgg cggacagcct gttcgacgtt cgcgggctcg agcaccttca
      721 ggcggcgctg gcgttgggtc gcggcgtaat cctcttcacg gggcacttca cgacgatcga
      781 gatctgcggg cgcgcgctga agcgcgccat gccgaacttc accgtcatgt atgcgccgcg
      841 cagcaaccct gtcatcgacg agatgcagcg gcgcgggcgt aggcgcgtct cgcacgattc
      901 cgttccgcac gacaacattc gaatgatgct gcgctgcctg aagcgcaacg cggcgatctg
      961 gtacgcgccc gaccagtact acgagggcgg cgccctcgtg ccgttcttcc acgagctcgc
     1021 gtgcacgaac gcggcgacga gcaagctcgc gcgcatgacg ggcgcgacgg tcgtgccgat
     1081 gtcgtaccgc cggctcgacg gcgaagcgcg ctacgaggtg cgcttccatg cgccgctagc
     1141 gggttttccg tccgacgacg agaccgccga taccgcgcgc ctcgttcgcg tgctcgaggg
     1201 cttcatccgt gccgcgcccg agcaatacct ctggaaccac cgccggttca aaggccggcc
     1261 ggcgccgctg ccggacctct acgcgcgtcc caaagtgtga cgcgaaacga ttcgcggctg
     1321 cgacgctaac cgccgaagag ccgcccgagc aggaaatgcg cgggcagata gacgagaagc
     1381 ggatacgcca gcattacgat gcagaaatgc accggcagcg gcagcgtgcg gcgcggcgga
     1441 tgaccgaacc cccgcaccca gttgacgttc ttctccggcg gcgcgagcca aagcgtcgcc
     1501 ggcagcacga cccaggcgag cagcgtctgt gcaacgaacg cccggctgtc atatccgtac
     1561 cggtaaacga gccaaagcgc gcccggcagc aaggcaaggt gaaacgccgc ggacagcgcg
     1621 cgtagagaga gcggaatgct cgcgtcgaac atgtaggttg ccagcgtcgt gacgccgagc
     1681 cccttgcgga tcgcgcccgt cacgaacgac acgagccata gcgtttcggg aatcaagacc
     1741 gcgagcgcca tcatgctgcc gagcaagccg tgctcgagcc acagcgcggc gcaaatgccg
     1801 agcaggccga tgtcggaaaa ccacaagaag ttggtgggac cgtacttgac gagatagacc
     1861 ggcacgacga cggcgacgag tatcgcgaac agcgtgcgca gagcgacgtc gatggcttcc
     1921 gccggctgcg cattgcccgt ctccgcgaaa gccgcgaatg aaagggtcaa cgcacagact
     1981 gcaagcgcaa cgatgacgcg gctacccaac gggcggcctc ggaagctcga tcatccctgc
     2041 gggcgtaagg gctgccgcga gcgtgacgtc gttgatgtcg cgatgcgccg tccacgacgc
     2101 ggtctcctgg ctgctcggct ccagccaaaa ccgttcgatc tcggcgacca tgggcgtata
     2161 tctctcgagc actcgcgtcg ttgcttgcag ccgtcgactc gcggccttgg ccgcgatgag
     2221 ctcgaggccg tgcaggccga gcgcgagccc gagctcgcgg aacggcaagc gcaaccggac
     2281 gggctgccgc ggtccgccgg cccgctcata atagtcgagc ccgtttgcgg cggaagcgag
     2341 aacgaacgtt gcgagggttt cgttgcgaaa cgcgcccgcg tccgtgagct gctcgatgcg
     2401 agccgcatcc atgagcaggc cgccaagccc gagcgggtcg gccgtggcga ggtcgctggc
     2461 ttctatcgtc gcgaccaaat cgtcggcgag ctcgcgaact tccggctgat gagcctcggg
     2521 gggcgcgagc tcggccgtcg cctgaagctg cacgtaagtg acatagccgt cgagcggatc
     2581 gtgctgaccg ggggtcggga cggccgcgcg gctgaggtcg atgctcatct tcc
//
LOCUS       DASSNN010000299         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_434209, whole genome shotgun sequence.
ACCESSION   DASSNN010000299 DASSNN010000000
VERSION     DASSNN010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434209"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VFL84_05460"
     CDS             <1..784
                     /locus_tag="VFL84_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7608102.1"
                     /translation="TLDLDVVFERVLVDAGNEKWVFSAGKDGVLWKHDLRTGEYLGHK
                     ETQFQNVWASFDRETGTPVYRPDILEHKVGEWIDGCPSTEGGKNWQAMSFHKPSRRMI
                     IPLSQSCISIRAQAIAQTPGGGSAGGADRRFYEMPGTDGNMGRLAAFNVDTLEQAWAI
                     QQRAPFLTGVLSTAGGVAFVGDLDREFKAVDVRNGRILWRTRLATSVQGFPVSFEVDG
                     RQYVAVTTGLGGGSPRLVPDLLEPEIDPPPRGHALYVFALPE"
     gene            846..1706
                     /locus_tag="VFL84_05465"
     CDS             846..1706
                     /locus_tag="VFL84_05465"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7608103.1"
                     /translation="MPSIEDISIIDSNTAKAKKVEHGLERTLCSPELCDSKNLTVYKR
                     TIEKGFELELKAGKDYHLVYVMSTAPKGTVSFKGQSHAAEEGAGVLLTPGESARFDAA
                     GADLDLLHMVVPKPPAEVEAGLPGGPGYFFNRKTLRGLSDASGGRVRRFCAESSVRLQ
                     DGSRLTPTNAIQAGEMHYNEGGSSPYHLHKGTPANPEGAAHCYMTFKGRGKVEVGDSA
                     QEIEPGTLVYFPPGVPHRLRAHGGTLDYFEIQAWRSFKTTILSEEPLGLKWYYEREKP
                     GAALVEWNQS"
     gene            1738..2679
                     /locus_tag="VFL84_05470"
     CDS             1738..2679
                     /locus_tag="VFL84_05470"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018485052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET7608104.1"
                     /translation="MTGSPAPVSSVLETIGRTPVVRLRRLVPPACADVVVKLEYFNPT
                     GSYKDRMALAMIEGAERSGRLRPGMRVVEYTGGSTGSSLALVCAMKGYRFHPLSSDAF
                     ATEKLDTMRAFGAELELVPSDGGRVTPQLFDRFKARVAELARDASTFWTDQFNNADAL
                     DGYANIGHELVEQVGHVDAFVGGVGTGGMLMGVSRVLKAHDPATRVVALEPSSSPFLT
                     TGKGGPHRVEGIATGFLPPHLSRGKHDEARAIDEDEGRDMARRAAREEGIFAGVSSGL
                     NLVGALQLARKLGPGKVVATVAVDTGLKYLAGDLYRS"
     gene            complement(2705..>3159)
                     /locus_tag="VFL84_05475"
     CDS             complement(2705..>3159)
                     /locus_tag="VFL84_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608105.1"
                     /translation="TLRELADALAVIKVLKKQLDQMRKQNAQGGGGHRQRADKTTKAR
                     ELLGEDAYGLMPAESHDNRVAARPSSGDDTRASGAYAHASSGDDDTSDGTINSLIANE
                     SWAERKANIGTGRAGAKVEAPEPELSKGEMLSPDLVFTDNDKEKDDER"
BASE COUNT          507 a         1042 c         1093 g          517 t
ORIGIN      
        1 gacactcgac ctcgacgtcg tcttcgagcg cgtgctcgtc gacgccggca acgagaaatg
       61 ggtgttctcg gccggcaagg acggcgtgct gtggaagcac gatctgcgaa ccggcgagta
      121 cctcggccac aaggagacgc agttccaaaa cgtttgggcg agcttcgacc gcgagacggg
      181 cacgcccgtc taccggcccg acatcctcga gcacaaggtg ggtgagtgga tcgacggctg
      241 cccgagcacg gaaggcggca agaactggca agcgatgagc tttcacaagc cgtcgcggcg
      301 catgatcatt ccgctgtcgc agagctgcat ctcgatccgc gcgcaagcga tcgcgcagac
      361 gccgggcggc ggcagcgccg gcggcgccga ccggcgcttc tacgagatgc ccggcaccga
      421 cggcaacatg ggccggctcg cggccttcaa cgtcgacacg ctcgagcaag catgggcgat
      481 ccagcagcgc gcgccgttct tgaccggcgt gctgtcgacg gccggcggcg tcgcgttcgt
      541 cggcgatctc gatcgcgagt tcaaagccgt cgacgtccgc aacgggcgca tcttatggcg
      601 aacgcggctc gcgacgtccg tgcagggttt tcccgtcagc ttcgaggtcg acggccggca
      661 atacgtcgcc gtgacgacgg gcttgggcgg cggcagcccg cgtctcgttc ccgacttgct
      721 cgagcccgag atcgatccgc cgccgcgcgg ccacgctttg tacgtgttcg cgttacccga
      781 gtgagcggtt tgcacgtatc atcgtcagcc catcgttgac ttaggcgctt gaggagcacg
      841 ctgccatgcc ttcgatagaa gacatctcga tcatcgattc gaacaccgcc aaagcgaaga
      901 aggtcgagca cgggctcgag cgcacgttgt gcagccccga gctctgcgac tccaagaact
      961 tgaccgtcta caaacgcacg atcgagaagg gctttgagct cgagctcaag gccggcaagg
     1021 actatcacct cgtctacgtc atgagcacgg cgccgaaggg caccgtctcg ttcaaggggc
     1081 agagccatgc cgccgaggaa ggcgccggcg tgttgctgac gcccggcgag tcggctcgct
     1141 tcgacgcggc gggcgccgac ctcgacttgc tgcacatggt cgtgccgaag ccgccggcgg
     1201 aagtcgaagc cggcttgccc ggcgggcccg gctacttctt caaccgcaag acgctgcgcg
     1261 gcttgagcga cgcgagcggc ggccgcgtgc ggcggttctg cgccgagtcg agcgtgcgtt
     1321 tgcaggacgg cagccgcctc acgccgacga atgcgattca ggcgggcgag atgcattaca
     1381 acgagggcgg ctcgtcgccg tatcacctgc acaagggcac gccggcgaac cccgaaggcg
     1441 ccgcgcattg ctacatgacg ttcaagggcc gcggcaaagt cgaggtcggc gacagcgcgc
     1501 aagaaatcga gccgggcacg ctcgtgtatt tcccgcccgg cgttccgcac cggctgcgcg
     1561 cgcacggcgg cacgctcgac tatttcgaga ttcaggcgtg gcgcagcttc aagacgacga
     1621 tcttgagcga ggagccgctc gggcttaagt ggtactacga acgcgagaag ccgggtgcgg
     1681 cgctcgtgga gtggaatcag agctagcctt tcggggcgcg gcgctccgga gactcgcatg
     1741 accggatcgc ccgcgcccgt gagctccgtt ctcgagacga tcggccgcac gcccgtcgtg
     1801 cggctgcggc ggctcgtccc gccggcttgc gccgacgtcg tcgtcaagct cgagtacttc
     1861 aacccgaccg gttcgtacaa agaccgcatg gcgctcgcga tgatcgaggg cgccgagcgc
     1921 agcggccggt tgcggcccgg catgcgcgtc gtcgagtaca cgggcggcag caccgggtcg
     1981 tcgctggcgc tcgtgtgcgc gatgaagggc tatcgattcc atccgctctc gtcggatgcg
     2041 ttcgccacgg agaagctcga cacgatgcgc gcgttcggcg cggagctcga gctcgtgccg
     2101 agcgacggcg gcagagtgac gccgcagctg ttcgaccgct tcaaggctcg cgtcgccgag
     2161 ctcgcgcgcg acgcgagcac gttctggacc gatcagttca acaacgccga cgcgctcgac
     2221 ggttacgcga acatcgggca cgagctcgtc gagcaggtag ggcacgtcga tgcgttcgtc
     2281 ggcggagttg gcacgggcgg catgctgatg ggtgtgtcgc gcgtgctgaa ggcgcacgac
     2341 cccgcgacgc gcgtcgtcgc gctcgagccg tcgtcgtcgc cgtttctgac gacgggcaag
     2401 ggcgggccgc atcgcgtcga aggcatcgcg accggcttct tgccgccgca cctgtcgcga
     2461 ggtaagcacg acgaagcgcg ggcgatcgac gaggacgaag gccgcgacat ggcgcggcgc
     2521 gccgcgcgcg aggaaggaat cttcgcggga gtgtcgtcgg gcttgaatct cgtcggcgcg
     2581 ctgcaactgg cgcgcaagct cgggccggga aaagtcgtcg cgaccgtcgc cgtcgacacg
     2641 gggctcaaat acctcgcggg cgatctctac cgaagctgag gcgcccggcg caaccgcgcg
     2701 agcgctagcg ctcgtcgtcc ttttccttgt cgttgtccgt gaacacgagg tccggcgaga
     2761 gcatttcgcc cttcgacagc tccggctccg gcgcctcgac cttcgcgccc gcccggccgg
     2821 ttccgatgtt ggctttccgt tccgcccagc tctcgttcgc gatcagcgag ttgatcgtgc
     2881 cgtcgctcgt gtcgtcgtcg ccggaggagg cgtgcgcgta ggcgcccgac gcgcgcgtgt
     2941 cgtcgccgga agaggggcgc gcggcgacgc ggttgtcgtg gctctcggcc ggcatcaggc
     3001 cgtaggcgtc ttcgccgagc agctcgcgcg ccttggtggt cttgtcggcg cgttgtcgat
     3061 gaccaccgcc accctgcgcg ttctgcttgc gcatctggtc gagctgcttc ttcaaaacct
     3121 tgatcacggc caacgcatcg gcgagctcgc gcaacgtgt
//
LOCUS       DASSNN010000300         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_291293, whole genome shotgun sequence.
ACCESSION   DASSNN010000300 DASSNN010000000
VERSION     DASSNN010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291293"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VFL84_05480"
     CDS             complement(<1..820)
                     /locus_tag="VFL84_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HET7608106.1"
                     /translation="MLDRTVGCAVLVLAAAVGGCGGGGGGGDAAGSSGGGSSSGWRAG
                     SFLPASSFAGKCVNPRTGTDPQTGTPYNEVRGTATDENNFLRSWSNDLYLWYNEIVDR
                     DPGQYATPDYFDLLRTTAVTASGAEKDKFHFTYPTAEWLSLSQSGVEAGYGALWAVVA
                     SFPPRRIVVAYTEPGSPAASAAGFTRGAEVLRIDGVDVVSSNTQAEVDAFVAGLYPES
                     TGEQHTFVTRDRFGTQRTVTLTSAAVTKTPVPVSSVIDFSGGRVGYLLFNDHVLT"
     gene            complement(892..1668)
                     /locus_tag="VFL84_05485"
     CDS             complement(892..1668)
                     /locus_tag="VFL84_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HET7608107.1"
                     /translation="MAALLAAENVVTHDRPHVIKGKVTSAEIVKRVDLTPEHFKIWLR
                     CAEPFTFEPGQYCTVGVEGVERPYSIVSSPREPLLELFVELIPPPLGHLTPLLHALPV
                     GAAVTLRPRAKGVFLMRPEFRNHVMVSTVTGVAPFISMLRYWLESGSDDRRMYVLEGA
                     SFLDELGYDDELRAIAAKHANVQFVPTCSRPNDPRNVGWKGQVGRVHTIVEHYVREWD
                     LDPAATCIYACGHPGMIEDLRGRYSGTGFTFEEERFWKSP"
     gene            1885..>2384
                     /locus_tag="VFL84_05490"
     CDS             1885..>2384
                     /locus_tag="VFL84_05490"
                     /inference="COORDINATES: protein motif:HMM:NF026164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HET7608108.1"
                     /translation="MLRLTAAVFMIAIAYGGFLAWQATSEFEGRRWDIPAQVYAAPLE
                     LFTGRALSAEDLVAELKRLGYREDPRLIGPGTYRVGLGRIEIATRGFSLAGDVEPEQL
                     VAVSFVGGRIAALRDSSGSRAAIVRLNPLLIGSLFAAHGEDRIIVAPDEIPTLLTDTL
                     KAVEDRR"
BASE COUNT          392 a          815 c          778 g          399 t
ORIGIN      
        1 ccgtcagaac gtggtcgttg aacagcaaat agccgacgcg cccgccgctg aagtcgatca
       61 cgctcgagac gggcacgggt gtcttcgtga cggccgccga cgtcaaggtg accgtgcgct
      121 gcgtcccgaa acgatcgcgg gtcacgaacg tgtgctgctc gccggtcgat tccggataca
      181 ggccggcgac gaacgcgtcg acctcggcct gcgtattcga gctcacgacg tcgacaccgt
      241 cgatccgcag cacttcggcg ccgcgcgtaa agcccgcggc cgatgccgcg ggcgagcccg
      301 gctccgtgta agccacgacg atgcgtcgcg gcggaaagct cgcgacgacg gcccagagag
      361 ctccgtagcc cgcctcgacg ccggactgcg acaggctcaa ccattcggcc gtcggatacg
      421 tgaaatggaa cttgtccttc tcggcgccgg acgccgttac ggccgtcgtc ctcagcaaat
      481 cgaagtagtc cggcgtcgcg tattgaccgg ggtcgcggtc gacgatctcg ttgtaccaga
      541 gatagagatc gttgctccac gagcgcagga agttgttctc atcggtcgcc gtgccgcgca
      601 cctcgttgta cggcgtgcct gtctgcgggt cggtgccggt gcgcggattg acgcatttgc
      661 cggcgaagct cgaagcgggc aagaacgatc cggcccgcca gccgctcgac gaaccgccgc
      721 cgctgctgcc ggcagcgtcg ccgccgcctc ctcctccgcc gcagccgccg acggctgcag
      781 ccagcacgag gaccgcgcac ccaaccgtcc tgtcgagcat agccatccgt tgccctccct
      841 cccgcaaacg gaactgtaac gcggaagact cgaacggtac atcggcggcg ctcacggcga
      901 cttccagaaa cgctcctcct cgaacgtgaa gcccgttccg ctgtagcgac cgcgtaaatc
      961 ttcgatcatg ccgggatgcc cgcacgcgta gatgcaggtg gccgccggat cgaggtccca
     1021 ctcgcggaca taatgctcga cgatcgtgtg cacgcggccg acttgcccct tccagcccac
     1081 gttgcgcggg tcgttcggcc gcgagcacgt tggcacgaac tgcacgttcg catgcttcgc
     1141 ggcgatcgcg cgcagctcgt cgtcgtatcc gagctcgtcc aagaagctcg cgccctcgag
     1201 cacgtacatg cgccggtcgt cgctcccgct ctcgagccag tagcgcagca tgctgatgaa
     1261 cggcgcgaca cccgtcacgg tcgagaccat gacgtggtta cggaactcgg gccgcatcag
     1321 gaacacgcct ttcgctcgcg gccgcagcgt cacggcggcg ccgaccggca acgcgtgcag
     1381 caacggcgtc aggtgtccga gcggcggcgg aatcagctcg acgaacagct cgagcaacgg
     1441 ctcgcgcggc gacgatacga tcgagtacgg ccgctctacg ccttcgacgc cgaccgtgca
     1501 gtactggccg ggctcgaacg tgaacggctc ggcgcagcgt aaccagatct tgaaatgctc
     1561 gggcgtcaga tcgacgcgct tgacgatctc ggcgctcgtg actttccctt tgattacgtg
     1621 cggccggtca tgcgtcacga cgttctccgc tgcgagcagc gccgccatag tacacggcgc
     1681 ccccgcgtgg cggtgcgcaa gaggtgccgg tgttacggtt cggttcaacc gaagtgccgg
     1741 agttacggtt gaaccgaagt gccggagtta cggttgaacc gaaccgtaac accggcaccg
     1801 cttgatgtgg cgcgccgact catgcaaggt gccggcatgg ccggccgcgc gcaacgtagc
     1861 aaagcctcgc gcatcagagg gttagtgctg cgcctcacgg ccgcggtctt catgatcgcg
     1921 attgcttacg gcggctttct cgcgtggcag gcgaccagcg agttcgaggg ccgccggtgg
     1981 gacattccgg cccaggtcta cgcggcgccg ctcgagctct tcacgggccg cgcgctgtcg
     2041 gccgaggact tggtcgccga gctcaagcgc ttgggctatc gcgaggaccc ccgcctcatc
     2101 ggcccgggca cgtatcgagt cgggttgggg cgcatcgaga tcgcgacgcg cggtttctct
     2161 ttggcgggcg acgtggagcc cgagcagctc gtcgcagttt cgttcgtcgg cggccgcatt
     2221 gccgcgctgc gcgactcgag cggcagccgc gcggcgatcg tgcgtttgaa ccctctcctg
     2281 atcggcagcc tgtttgcggc gcacggcgag gaccgcatca tcgtcgcgcc cgacgagatc
     2341 cctacgctct tgaccgacac tctgaaggcc gtcgaggatc gccg
//
LOCUS       DASSNN010000301         5352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1569198, whole genome shotgun sequence.
ACCESSION   DASSNN010000301 DASSNN010000000
VERSION     DASSNN010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569198"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /locus_tag="VFL84_05495"
     CDS             <1..1733
                     /locus_tag="VFL84_05495"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7608109.1"
                     /translation="QERGELKHHGAEAELMLGGLYLSYGQHQLAGEIFERVLEQSVDP
                     ALHDRAWYFLAKIWQQRGYLPEAEAALARIRGELPEELEPERQMLHAQVLMEQGRFAD
                     ALAALEAWRRPGDAWVGYAKYNIGVSLVRLGQVEQGARVLDEVGRLDPENPAFDALRD
                     KANVALGYAWLQASHPVEAKPSLQRVRLDGPFSNKALLGVGWSDAETDDYRAALAPWL
                     VLRDRSLLDSAVQESMLAVPYAFAQLGADKQAADHYVDAIEAFSGEIVRLTRSIEAIE
                     NGELITELLGKHEDAADEASGWYWRLERIPDTVESRYLYELLASNRFQEGLKNYRDLV
                     ELNRNLGRWTESLSAFDDILDTRQRAYEQRLPAIDASLESVDLAAMTQRRVELESRLQ
                     AIERTEDVVALGTAKQQQNWQLIEAMEQKLALLPHDAASDEMRAKHRFLRGLLLWDLR
                     RDYKARLWAEHKALGDLDRQLREAQRRHHQVASSRDDWPEKFTALTARIGALRPRVNG
                     LKESAQVALLRQQEFLQDIAVEELKAQRDRLNVYQIQARFALASIYDRAAARVEPAGE
                     AETALAGGGQ"
     gene            1730..4555
                     /locus_tag="VFL84_05500"
     CDS             1730..4555
                     /locus_tag="VFL84_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7608110.1"
                     /translation="MSNAPLLVLLAASAIWPFGRDRDREKPVEPTGTIKDLERADVEV
                     DTGARIVGSEAKAMESYRLFLDVASDDPVLRAEAMRRLADLQLETADAEELATNVQAL
                     GTLGSTIEMYEQLLKSYPNYAKNDLVLYQLARAYEAEGRIDESLATLDKLIATYPRTT
                     HLDEAQFRRGETLFVQKRYRDAERAYAYVLGVGDGSPFYEQSLYKHGWALFKQQEYDD
                     SMESFFTLLDRQLGTDNNASGDRDPAVLYAAMGRAQQELVDDTFRVLSIGFSYLDGPE
                     SISRYFNRHGPRPYSFIAYTNLGDLYLEQQRYQDAADAYHAFVELDPYHAKAPLLQVE
                     VIEAFKKGEFADLVLAGKENFVETYGPGSPYWQRFTFEQQPEVVAHLKSNVTDLAAYH
                     HAEAQKSHDAREYAAAARWYRSYLQSFPDDANAPHTNFLLAEVLYESGNFGDAAAEYE
                     RTAYAYPFHEDSGEAGYAALLAYAKHEDRLTGAPRAEWHRRAIDSALKFSETYPAHPQ
                     AATVQTDAAEKLYALDEFARARDVGRQVVARTPPVEPKLQRTAWTVVAHSEFDLQDFA
                     AAETAYVQLLGLMPAADPQRGEITERVASSIYKQGEQARTAGQLDVAVEHFLRVSRAA
                     PTSPVRATAEYDAGAALIQIGDWQRATAVLENFRATFPNHALAADVTTKLAVAYVESG
                     NSARAASEFERIADGDGPVEVKREALWRSAELYEKTGQSAAATTAYGRFVERYPSPVA
                     AAVEARQKLIELSGAAGNRAERTRWMRDLVVADAAAGAERTDRTRYLAAKAQLELAAP
                     ARDAFLRTRLVVPLDKSLEAKQQRMQEALTEYGKAADYGIAEVTTAANYEIAELYHAL
                     SKDLYASERPPELTADELEQYDILLEEQAFPFEEEAIQLHETNAARTTEGIYDEWVRK
                     SFTALGELSPGRYAKAEIGETVVEAIR"
     gene            4536..5321
                     /locus_tag="VFL84_05505"
     CDS             4536..5321
                     /locus_tag="VFL84_05505"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7608111.1"
                     /translation="MSKRFAELAASPFVLRSFAAGALALTLAACGSSTPRQADVEAPR
                     EVAAPAVTRAEPAAESAPAPERRRDRRSERRAADEASADAAAAVLEPVPEAAAAAHER
                     ALVAMRAQNWVEAELELEQLVLEHPEYPGPHVNLALVYLNDGRRDDARKEIDLALGVD
                     PGHAAANTQLGILLREEGKFEAAEAAYRRALETDSDHALAHYNLGVLLDIYLRRTAEA
                     LEQYEAYQASLSQPDETVGRWIIDLRRRAGNGGAARVAQGESP"
BASE COUNT          914 a         1838 c         1845 g          755 t
ORIGIN      
        1 cgcaagagcg cggtgagctc aagcatcacg gcgccgaggc agagctcatg ctcggcggcc
       61 tgtatctgtc gtacggccag catcaactgg ccggcgagat cttcgaacgc gtactcgagc
      121 aaagcgtgga tcctgccttg cacgaccgcg cgtggtattt cctcgcgaaa atctggcagc
      181 agcgcggcta tctgccggag gccgaagcgg cgctcgcgcg catccgcggc gagctgcccg
      241 aggagctcga gcccgagcgg cagatgctcc acgcacaggt tctgatggaa cagggccggt
      301 tcgccgacgc tctggccgcg ctcgaggcgt ggcgccgccc cggcgacgcg tgggtcggct
      361 acgcgaagta caacatcggc gtctcgctcg tgcggttggg gcaagtggaa cagggcgcgc
      421 gtgtgctcga cgaagtcggg cggctcgatc ccgagaaccc cgcgttcgac gcgctgcgcg
      481 acaaggcgaa cgtcgcgctc ggttacgcgt ggctgcaagc gtctcatccc gtcgaggcga
      541 aaccttcgct gcagcgcgtg cgcctcgacg gcccgttctc gaacaaggcg ctgctcggag
      601 tcggctggtc cgatgccgag accgacgact accgtgcggc gctcgcccct tggctcgtgc
      661 tgcgcgaccg cagcctgctc gactcggccg tccaggagtc gatgctggcc gtgccgtatg
      721 cgttcgcgca gctcggcgcc gacaagcagg ccgccgacca ttacgtcgac gcgatcgagg
      781 cgttcagcgg cgagatcgtg cgtctcacgc ggtcgatcga ggccatcgag aacggcgagt
      841 tgatcacgga gctgctcggc aagcacgagg acgccgccga cgaggcgtcg ggctggtact
      901 ggcggctcga gcgcatcccc gacaccgtcg agagccgcta tctctacgag ctgctcgcga
      961 gcaaccgctt ccaagagggg ctcaagaact accgcgactt ggtcgagctc aatcgcaact
     1021 tgggccgctg gaccgagagc ttgagcgcgt tcgacgacat cctcgacacg cgccagcgcg
     1081 cgtacgagca gcggctgccc gcgatcgacg cgagcctcga gagcgtcgat ctcgccgcga
     1141 tgacgcagcg ccgcgtcgag ctcgagtctc gcctgcaggc aatcgagcgc accgaggacg
     1201 tcgtcgcgct cggcacggca aagcagcagc agaactggca gctcatcgag gccatggagc
     1261 agaagctcgc gttgctgccg cacgatgccg cgagcgacga gatgcgcgcc aagcatcgct
     1321 tcctgcgcgg gctgttgctc tgggatttgc gccgcgacta caaggcgcgg ctctgggccg
     1381 agcacaaggc gctcggcgat ctcgaccggc agctgcgcga ggcgcaacgc cgccaccatc
     1441 aggtcgcaag ctcgcgcgac gattggccgg agaagttcac ggcgctgacc gcgcgcatcg
     1501 gcgccttgcg gccgcgcgtg aacgggctca aggagtcggc ccaggtcgcg ttgctgcggc
     1561 agcaggagtt cctgcaggac atcgccgtcg aagagctcaa agcgcagcgc gatcggctga
     1621 acgtgtatca aatccaagca cggttcgcgc tcgcgtcgat ctacgaccgt gccgcggcgc
     1681 gagtcgagcc cgccggcgaa gcggagacgg cgctcgcggg aggcggccaa tgagtaacgc
     1741 gccgttgctc gtgctgctcg cggcgtcggc gatctggccg ttcggccgcg atcgcgatcg
     1801 cgagaagcca gtcgagccga ccggcacgat caaggacctc gagcgcgccg acgtcgaggt
     1861 cgacacgggg gccaggatcg tcggcagcga agcgaaagcc atggagagct atcgcttgtt
     1921 cctcgacgtc gcgtccgacg atccggtgct gcgcgcggaa gcgatgcgcc ggctcgccga
     1981 cctgcagctc gagacggccg atgccgagga gctcgcgacg aacgtgcagg cgctcggcac
     2041 gctcggcagc acgatcgaga tgtacgagca gctgctgaaa tcgtatccga actacgcgaa
     2101 gaacgatctc gttctctatc agctcgcgcg cgcctacgag gccgagggcc gcatcgacga
     2161 gtcgctcgcg acgctcgaca agctcatcgc cacgtatccg cgcacgacgc atctcgacga
     2221 ggcgcagttc cgccgcggcg agacgctgtt cgtgcagaag cgttaccgcg acgcggagcg
     2281 cgcgtacgcg tacgtgctcg gcgtcggcga cggctcgccg ttctacgagc aatcgctcta
     2341 caagcacggt tgggcgctgt tcaagcagca ggaatacgac gacagcatgg agtcgttctt
     2401 cacgctgctc gaccggcagc tcggcaccga caacaacgcg agcggcgatc gcgacccggc
     2461 cgtgctctac gcggcgatgg gccgcgcgca gcaagagctc gtcgacgaca cgttccgcgt
     2521 gctgtcgatc ggcttctcgt acttggacgg tcccgagtcg atctcgcggt acttcaaccg
     2581 ccacggcccg cggccgtact cgttcatcgc gtatacgaac ctgggcgacc tctatctcga
     2641 gcagcagcgc tatcaagacg ccgccgacgc ctatcacgcg ttcgtcgagc tcgacccgta
     2701 tcacgccaaa gcgccgctgc tgcaggtcga ggtcatcgag gcgttcaaga agggcgagtt
     2761 cgccgacctc gtgctcgccg gcaaagagaa cttcgtcgag acctacggcc ccggcagccc
     2821 gtactggcag cggttcacgt tcgagcaaca gcccgaagtc gtcgcgcatc tgaagagcaa
     2881 cgtcaccgac ctcgcggcgt atcaccacgc cgaggcgcag aagtcgcacg acgcgcgcga
     2941 gtatgcggcg gcggcgcgct ggtaccgttc gtacttgcag tcgttccccg acgacgcaaa
     3001 cgcgccgcac acgaacttct tgctcgccga ggtgctctac gagagcggca acttcggcga
     3061 cgcggcggcc gaatacgagc gcaccgccta cgcctatccg ttccacgagg actcgggcga
     3121 agcgggttac gcggcgttgc tcgcgtatgc gaagcacgag gaccggctga ccggcgcgcc
     3181 gcgcgcggag tggcaccgcc gcgccatcga cagcgcgctg aagttctccg agacgtaccc
     3241 cgcgcacccg caggcggcga ccgtgcagac cgacgcggcc gagaagctct acgcgctcga
     3301 cgagttcgcg cgcgcgcgcg acgtcggccg ccaggtcgtc gcgcgcacgc cgccggtcga
     3361 gccgaagctg cagcgcacgg cgtggaccgt cgtcgcgcac tcggagttcg acctgcaaga
     3421 tttcgcggcg gccgagacgg cttacgtgca gctcttgggc ctcatgcccg ccgccgatcc
     3481 gcagcgcggc gagatcaccg agcgcgtcgc gtcgtcgatc tacaagcagg gcgagcaggc
     3541 gcgcacggcc ggccagctcg acgtggccgt cgagcacttc ttgcgcgtgt cgcgtgccgc
     3601 gccgacgtcg cccgtgcgcg cgacggccga gtacgacgcc ggcgccgcgc tgatccagat
     3661 cggcgattgg caacgcgcga ccgcggtgct cgagaatttt agggccacgt ttccgaacca
     3721 cgcgctcgcc gccgacgtga cgacgaagct cgcggtcgcc tacgtcgagt cgggcaacag
     3781 cgcgcgcgcg gccagcgagt tcgagcgcat cgccgacggc gacgggccgg tcgaggtcaa
     3841 gcgcgaggcg ctctggcgct cggccgagct ctacgagaag acggggcaga gcgccgccgc
     3901 gacgacggcg tacggccggt tcgtcgagcg ctacccgtcg ccggtagcgg cggccgtcga
     3961 agctcggcag aagctgatcg agctctccgg cgctgccggc aatcgcgcgg agcgcacgcg
     4021 ctggatgcgc gatctcgtcg tcgcggacgc ggctgcgggc gccgagcgca cggatcgcac
     4081 gcgttacctc gcggccaagg cgcagctcga gctcgccgcg ccggcgcgcg acgcgttcct
     4141 ccgcacccgc ctcgtcgtgc cgctcgacaa gagcctcgaa gcgaaacagc agcgaatgca
     4201 ggaagcgctc accgaatacg gcaaggcggc cgactacggc atcgccgagg tcacgacggc
     4261 cgcgaactac gagatcgccg agctctatca cgcgctcagc aaggatctct acgcgtcgga
     4321 gcggccgccc gagctcacgg ccgacgagct cgagcaatac gacatcctgc tcgaggagca
     4381 ggcgttcccg ttcgaggagg aagccatcca gctgcacgag acgaacgccg cgcgcacgac
     4441 ggaaggcatc tacgacgaat gggtgcgcaa gagcttcacg gcgctcggcg agctctcgcc
     4501 cgggcgctac gccaaagccg agatcggaga gaccgttgtc gaagcgattc gctgagcttg
     4561 ccgcatcgcc gttcgtgctg cgttcgttcg cagccggcgc gctcgcgttg acgctcgccg
     4621 cgtgcggctc gtcgacgccg cggcaggccg acgtcgaggc gccacgcgaa gttgcggcgc
     4681 ccgcggtcac gcgcgccgaa cccgccgcgg aatctgcacc cgcgccggag cggcggcgcg
     4741 accggcgcag cgagcggcgt gcggccgatg aggcgagcgc ggacgcggcc gccgcggtcc
     4801 tcgagccggt gccggaagcc gcggccgccg cccatgagcg cgcgctcgtc gcgatgcgcg
     4861 cccagaattg ggtcgaagcc gagctcgagc tcgagcagct cgtgctcgag catccggagt
     4921 atcccggccc gcacgtcaac ctcgcgctcg tctatttgaa tgacggacgc cgtgacgacg
     4981 cgcgcaagga gatcgacctc gctctcggcg tggaccccgg gcacgcggct gccaacacgc
     5041 agctcggcat cctgctgcgc gaggaaggca agttcgaggc tgccgaagcg gcctatcggc
     5101 gcgcgctcga gacggactcg gaccacgcgc tcgcgcacta taacttgggc gtgctgctcg
     5161 acatttattt gcggcgcacc gcggaagcgc tcgagcagta cgaggcgtat caggcgtcgc
     5221 tgtctcagcc cgatgaaacc gtggggcgtt ggatcatcga tttgcggcgc cgcgccggca
     5281 acggcggcgc agcgcgagtc gcgcaagggg agagcccgtg aaagcaatca tgacgatcgt
     5341 gttggcgttg ct
//
LOCUS       DASSNN010000302         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1569222, whole genome shotgun sequence.
ACCESSION   DASSNN010000302 DASSNN010000000
VERSION     DASSNN010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569222"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFL84_05510"
     CDS             complement(<1..258)
                     /locus_tag="VFL84_05510"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HET7608112.1"
                     /translation="MDAALDAAAALLFRASALAIGIVLLAAAFHKLRDFAAFRASIEG
                     YRLLPGAQVVVVAALMPVLEALAGVALIVDGLRVAGAWLAFA"
     gene            complement(258..1403)
                     /locus_tag="VFL84_05515"
     CDS             complement(258..1403)
                     /locus_tag="VFL84_05515"
                     /inference="COORDINATES: protein motif:HMM:NF018172.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HET7608113.1"
                     /translation="MHRPRIIGWLAAVLCIAPALAADPPNEQVTTQPLPPANAHRVYL
                     ADFTINHVMDGRLNVIDGEKLKLQGIVATGFAGLPTLSPDRSELYVATTYYTRLNHGD
                     RVDVVDVYDLATLGHKAEIPIPPKHAMALPYQGVIRTSVDGRLLFVQNATPAQSVSVV
                     DLRTRQFVAEVQTPGCWIILPSQTVAPRFTTLCGDGTLLTVTLDDDGKQKSMKRSERF
                     FDPDRDPLFVQAENVGDRYFFVSFNGRIHTADVGGEVARFEPSWPLVSAPEARAGWRP
                     GGYQPLALHQRSGRLYVAMHARGSEGSHKNPAKEIWVFDVAAKKRVARAPGSNAIAIA
                     TSRDDTPRLYAIDGVRMGLAVYDTAPRLVPRRRMEGVAEAGTLLVMH"
     gene            complement(1564..>2026)
                     /locus_tag="VFL84_05520"
     CDS             complement(1564..>2026)
                     /locus_tag="VFL84_05520"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HET7608114.1"
                     /translation="ACIVHVVRLLLWRGWRVRGHPLVSAMHVGYAWLVGAFGLAAYSA
                     LTGGTWPRAWLHAFTVGAVGTMMLALMPRVALRHTGRAPANAPFVAASCAAMTLAAAL
                     RLVFGLPAASAWYGAVAAALWAACFVGYAIVYAPMLMQPSLPRPAAPAPIA"
BASE COUNT          361 a          693 c          673 g          299 t
ORIGIN      
        1 cgcgaacgcg agccacgcac cggcaacgcg caagccgtcc acgatcaacg ccacgccggc
       61 gagtgcctcg agcacgggca tcagcgcggc gaccacgact acttgcgctc cgggaagcag
      121 ccgataacct tcgatgctcg cgcggaatgc cgcgaagtcc cggagtttgt ggaatgccgc
      181 agcgagcagc acgatgccga ttgccagcgc gctcgcccgg aacagcaagg ccgctgctgc
      241 atcgagcgcc gcgtccatca atgcatcacc agaagcgtgc ccgcctcggc gacgccttcc
      301 atccgtcgtc gcggaacgag acgcggcgcc gtgtcgtaga cggcaagccc catcctgacg
      361 ccgtcgatcg catagagcct tggcgtgtcg tcgcggctcg tcgcgatcgc gatcgcgttg
      421 ctccccggcg cacgtgcgac gcgcttcttc gccgcgacgt cgaagaccca gatttccttc
      481 gcgggattct tgtggctccc ctcgctgccg cgcgcgtgca tggcgacgta caaccgcccc
      541 gagcgctgat gcaacgcgag cggctggtat ccgcccggcc gccagccggc ccgcgcttcg
      601 ggagccgaga cgagcggcca ggacggctcg aatcgcgcca cttcaccgcc gacgtccgcc
      661 gtgtggattc ggccgttgaa cgaaacgaag aagtagcgat cgccgacgtt ctcggcctgc
      721 acgaacagcg gatcgcggtc ggggtcgaag aagcgctcgc tgcgcttcat cgacttctgc
      781 ttgccgtcgt cgtcgagcgt gacggtcagc agcgtcccgt cgccgcacag tgtcgtgaac
      841 cgcggcgcga ccgtctgcga cggcaggatg atccagcacc ccggtgtttg cacttcggcg
      901 acgaactgcc gcgtgcgcag gtcgacgacg ctcaccgact gcgccggcgt tgcgttctgg
      961 acgaacagca accggccgtc gacggacgtg cgaatgacgc cttgatacgg caacgccatc
     1021 gcgtgcttcg gcggtatcgg gatctccgcc ttgtgcccga gcgtcgcgag atcgtagacg
     1081 tcgacgacgt cgacccggtc gccgtggttg agccgcgtgt aataggtggt cgccacgtac
     1141 agctcggagc ggtcgggcga cagcgtcggg agtccggcga accccgtcgc gacgatcccc
     1201 tgcagcttca gcttctcgcc gtcgatgacg ttgagccgcc cgtccatcac gtgattgatc
     1261 gtgaagtcgg caaggtagac gcggtgcgcg ttcgccggag gcagaggctg tgtcgtcacc
     1321 tgctcgttcg gcggatccgc ggcgagtgcc ggcgcaatgc acaacacggc ggcaagccaa
     1381 ccgatgattc gcggtcgatg catcgtgctc gtgccccctt gcctttgacg caccttggcg
     1441 gcgctctgga atgagccgag tgtagagagc gcacagcggc gtgcgttatc ccaaatcggc
     1501 aagccggtcc tgccggactt gctgcagatc aagaaatccg cgtgtgggtt tcgcaggcga
     1561 acctcacgcg attggtgcgg gcgccgcagg tcgaggcaga ctcggctgca tgagcatcgg
     1621 tgcatagacg atcgcgtagc cgacaaagca cgccgcccat agagcggcgg cgacagcgcc
     1681 ataccacgcc gatgctgcgg gaagcccaaa aacgagccgc agtgccgccg ccaacgtcat
     1741 tgcggcgcac gaggccgcca cgaagggagc gttcgcaggc gcgcgcccgg tgtgccgcag
     1801 cgcaacgcgc ggcatcaggg ccagcatcat cgtacccacg gcgccgacgg tgaacgcatg
     1861 cagccacgcg cggggccacg tgcctccggt cagcgccgag tacgctgcga ggccaaacgc
     1921 gccgacgagc caggcgtagc cgacgtgcat ggccgacacg agcggatggc cacgaacgcg
     1981 ccaaccacgc caaagcagga gccgaacgac atggacgatg caggca
//
LOCUS       DASSNN010000303         4777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_798090, whole genome shotgun sequence.
ACCESSION   DASSNN010000303 DASSNN010000000
VERSION     DASSNN010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798090"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VFL84_05525"
     CDS             complement(<1..689)
                     /locus_tag="VFL84_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7608115.1"
                     /translation="MKPAPPLRVLSSMAGRKLLGDMIAKYEAASEQRVALECVGGVDA
                     AKRVRAGEALDVAVLSREAIDELIGAGRIAPGSRVDLWVSAIGVAVRAGARRPDIGSA
                     AAVKAAVLAARKVGYSTGPSGKYLAKLFAGWDSDGTLEERITVAPPGVPVASLVARGD
                     IDIAFQQLSELAGDGVEVLGPLPEEIQMITTFSGGVALSSAQPEAARALLKFFGSPAL
                     KAVKQAHGMDAP"
     gene            742..1029
                     /locus_tag="VFL84_05530"
     CDS             742..1029
                     /locus_tag="VFL84_05530"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET7608116.1"
                     /translation="MHAITDLPRRHWLTVDDYYRMAEVGILDDDARVELIDGEIIDMA
                     PPGSPHAATVSYLTEVFMSAVGGRAAVRVQNPVRLSKYSEPQPDLSLLLVA"
     gene            1087..2190
                     /locus_tag="VFL84_05535"
     CDS             1087..2190
                     /locus_tag="VFL84_05535"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HET7608117.1"
                     /translation="MRIFTSAVLSGVLAWSAVDEPAHAQADARDTGDLKLFRFELDND
                     IFVRSDDAFTAGWSVQLHSPLLDEWPPGLGGWIGRVPGLDDDGAGGRVVRRAWGITQL
                     IITPGNITIARPQPNDAPWAGMLGAYVSWSAYDNDRLAALQAYVGCVGSCSHAEEVQK
                     LMHNSFGFGDEPRGWPNQLADRPLLNLNYEYRHKLWARADFDPRGWSQDLSAGGQVGL
                     GSFATYAQAWLEYRFGWDVPEGFTNLSDPPALGVALDPTYVEPGVPARPRTWRPYFSV
                     VARVRALDRFAPLLGGETDNGGFHPAIESAPGDKQVLVGIHVARVPLAFHLTYYRFFG
                     DDDFAARTGGELDWVNFSFERRFARRTVRAPRG"
     gene            complement(2208..4229)
                     /locus_tag="VFL84_05540"
     CDS             complement(2208..4229)
                     /locus_tag="VFL84_05540"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7608118.1"
                     /translation="MMFDVLLEAALRAAVIAAVAAAVLWALRVRAAAVRHRVWTVVMI
                     AMLALPIAIAWAPDVSLRVLPPAHSAAPVAPEALAPLSNSFAAAVTATEPATAGTVAL
                     TPAPSPSWSWRAWLAAIYLAGAAALLARLAIGTVRVRLLAREAAVVNGELTSARIATP
                     FTFGLLRPKILLPEGWQRWPAARLAVVLDHERAHVARRDPLVQWLALLNRAVFWFHPL
                     AWWIERHLAALAEEACDAAVLARGHSPADYSEHLLELARPANHRPLPNLAGMPMLGSG
                     LATRIPKILEGGIALPGSRSAAAGAAALATLAAAVLGTVTLAQEPALPRTQDLPSPVR
                     EQVEKFDSLPPEQQRALIRDLQRNLQPAQLQAVQQMLQGASRNLLEVYQLALANDPAV
                     RSAEAAYRAATDARPQVQSAIALATADYEAARQQLLIRVAEPYFDVFAAARALALQEQ
                     DREALSRQLAQTQRRFDVGLVGVTDVQEAQGGYDQAVANALTAQRALATAQDALRNVV
                     GEPVGELRPLVKDLPLQPPDPGNEEAWIETALKANPVLVSTRLRAESGEDDANARRAR
                     EDLERVARVIESETRAAYLGVVSEMSRVLALEQSVRSNEVALEATEAAFEVGTRSTVD
                     VLRAQSTLSQSETVYERSRYDYALSVLRLRGAAGDLTAQGLEETKSWFD"
     gene            complement(4226..4633)
                     /locus_tag="VFL84_05545"
     CDS             complement(4226..4633)
                     /locus_tag="VFL84_05545"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HET7608119.1"
                     /translation="MTKRSKKARVRAPLTELESEVMKAVWSGTRSSVEDVHRIVARKY
                     DLKETTVRTLLRRLEHKGYLEHEAEGRAYLYRAAEPAGHLAARAVRQIIDRFCQGSVE
                     ELVSGMVDAKMLSDEEFERLEKYVRSRRRRESP"
BASE COUNT          705 a         1657 c         1651 g          764 t
ORIGIN      
        1 ggcgcgtcca tgccgtgcgc ttgcttgacg gccttgagcg ccggagagcc gaagaacttg
       61 agcagcgcgc gtgcggcctc cggttgcgcc gaggagagcg cgacgccgcc ggaaaacgtc
      121 gtgatcattt ggatttcttc cggcagcgga cccagcacct cgacgccgtc gccggcgagc
      181 tcgctcaact gctgaaacgc gatgtcgatg tcgccgcgcg cgacgaggct cgcgaccggc
      241 acgccgggcg gcgcgaccgt gatgcgctct tcgagcgtgc cgtcgctgtc ccagcccgcg
      301 aacagcttcg cgagatactt gccgctcggg cccgtcgaat agccgacttt gcgcgccgcg
      361 aggaccgccg ctttgaccgc cgccgccgag ccgatgtccg gccgccgcgc gcccgctcgg
      421 acggcgacgc cgatcgccga gacccagagg tcgacgcggc tacccggcgc gattcgcccg
      481 gcgccgatca actcgtcgat ggcttcgcgc gacagcaccg cgacgtcgag cgcctcgccg
      541 gcgcgcacgc gcttcgcggc atcgacgccg ccgacgcact cgagcgccac gcgctgctcc
      601 gacgcggctt cgtacttcgc aatcatgtcg ccgagcagct tgcggcccgc catcgacgag
      661 aggacgcgga ggggaggcgc gggtttcacg gcgtgagaat accccgttca ctcgcattcg
      721 cgcaccgcct atagtgaaac catgcacgca atcacggatc tgccgcgtcg tcactggctc
      781 acggtcgacg attactaccg catggcggaa gtgggcatcc tcgatgacga cgcgcgcgtc
      841 gagctgatcg acggcgagat catcgacatg gcgccccccg gcagcccgca cgccgccact
      901 gtgagctacc tcacagaggt cttcatgagt gcggtgggag gaagggccgc cgtgcgcgtc
      961 caaaatccgg tgcggctcag caagtactcg gagccccagc ctgatctctc gcttttgctc
     1021 gtggcttgag tcttgcacgc cgaaattccg acgccgaccc aggattcgcg gcgtcggagg
     1081 ggcggcgtgc gaattttcac ttcggcggtt ttgagcggcg tgctcgcgtg gtcggcggtt
     1141 gatgaaccgg cgcacgcgca ggccgacgcg cgcgacaccg gcgacctcaa gctgttccgc
     1201 ttcgagctcg acaacgacat cttcgtccgc tcggacgatg cgttcacggc cggttggagc
     1261 gtccagctgc attcgccgct gctcgacgaa tggccgcccg gcctcggcgg ctggatcggc
     1321 cgggttccgg ggctcgatga cgatggcgcg ggcgggcgcg tcgtgcgccg cgcgtggggg
     1381 atcacgcagc tgatcatcac gcccggcaac atcacgattg cgcggccgca gccgaacgat
     1441 gcgccgtggg ccggaatgct cggcgcctac gtgtcctggt cggcgtacga caacgacagg
     1501 ctcgcagcgc tgcaagccta tgtcggttgc gtcggctcct gctcgcacgc cgaggaggtg
     1561 caaaagctca tgcacaacag cttcggcttc ggcgacgagc cgcgcggctg gccgaaccag
     1621 ctcgccgatc ggccgctgtt gaacctgaac tacgaatacc ggcacaagct ctgggcgcgc
     1681 gccgacttcg atccgcgcgg gtggtcgcaa gacctctcgg ccggcgggca ggtcggtctc
     1741 ggctcgttcg cgacgtacgc gcaggcgtgg ctcgagtacc gcttcggctg ggacgtgccc
     1801 gaaggattca cgaatctctc cgatccgccc gcgctcggcg tggcgctcga tccgacctac
     1861 gtcgagcccg gcgttcccgc gcggccacgc acgtggcgcc cgtatttcag cgtcgtggcg
     1921 cgcgtccgcg cgctcgatcg gttcgcgccg cttttaggcg gcgagaccga caacggcggc
     1981 tttcaccccg cgatcgagtc ggcgcccggc gataagcagg ttctcgtcgg catccacgtc
     2041 gccagagtgc cgctcgcgtt ccatctgacg tattaccgct tcttcggcga cgacgacttc
     2101 gcggcgcgca cgggcggaga gctcgactgg gtgaatttct cgttcgagcg ccgcttcgcg
     2161 cggcgcacgg ttcgcgcgcc gcgcggctga gctcggtgca gggccgctca gtcgaaccag
     2221 ctcttcgtct cttcgaggcc ctgagccgtc agatcgcccg cggcgccgcg gaggcgcagc
     2281 acgctcagtg cataatcgta gcggctgcgc tcgtacaccg tctcgctctg actgagagtg
     2341 ctctgcgccc tgagcacgtc caccgtgctt cgcgtgccga cctcgaaggc ggcttcagtc
     2401 gcctcgagcg cgacctcgtt cgaccgcacg gattgctcga gtgccagcac gcgtgacatt
     2461 tcggagacga cgccgaggta ggccgcgcgc gtctcggact cgattacacg cgcgacgcgt
     2521 tcgagatcct cgcgggctcg ccgggcgttg gcatcgtcct cgccgctctc ggcgcggagc
     2581 cgcgtcgaga cgagcacggg gttcgccttc agggccgtct cgatccaggc ttcctcgttg
     2641 ccgggatccg gtggctgcaa cggcaggtct ttgacgagcg gcctcaactc gccaacgggc
     2701 tcgccgacga cgttacgcag ggcgtcctgc gccgtggcga gcgcgcgctg cgccgtcagt
     2761 gcgttcgcga ccgcctgatc gtaaccgcct tgggcttcct gtacgtctgt gactccgact
     2821 agcccgacat cgaagcgccg ctgagtctgt gcgagctgcc gcgagagcgc ttcacggtcc
     2881 tgctcttgga gcgcgagcgc acgcgcggcg gcgaacacgt cgaagtaggg ctcggccacg
     2941 cggatcagca gctgctgccg cgcggcctcg tagtcggccg tggctagagc gatcgcgctc
     3001 tgtacctgcg gccgcgcgtc ggtggccgcg cgatacgcgg cctcggcact gcgcacagcc
     3061 ggatcgttcg cgagcgcgag ctgatagacc tcgaggaggt tccgcgacgc gccctgcagc
     3121 atctgttgaa cggcttgcag ttgcgccggc tgaaggttcc gttgcaggtc gcggatcagt
     3181 gcccgctgtt gctccggcgg cagcgaatcg aacttctcga cctgctcgcg caccggactc
     3241 gggaggtctt gcgtgcgcgg caaggccggc tcttgcgcga gcgtgaccgt gccgagcacg
     3301 gccgcggcga gcgtcgcgag cgccgctgcg ccggccgcgg ccgagcgcga gccgggcagt
     3361 gcgatgccgc cctcgagaat cttcgggatg cgcgtcgcga gcccgctgcc gagcatcggc
     3421 atgccggcaa ggttcggcag cggtcgatga ttcgccgggc gcgcgagctc gagcagatgc
     3481 tccgagtaat ccgcgggact gtggccgcgt gcgagcacgg ccgcgtcgca cgcttcttcg
     3541 gccaatgcgg cgaggtgccg ctcgatccac caggcgagcg ggtgaaacca gaagacggcg
     3601 cggttcagca gcgcgagcca ttgcacgagc ggatcgcgcc gtgcgacgtg ggcgcgttcg
     3661 tgatcgagca cgacggcgag gcgcgcggcg ggccagcgtt gccagccctc cggcaacagg
     3721 atcttgggtc taagcaaccc gaacgtgaac ggcgttgcga tgcgcgcgct cgtcagctca
     3781 ccgttcacga ccgccgcttc gcgagcgagc aaacgaacgc gcacggtgcc gatcgcgagc
     3841 cgtgcgagca acgcagcggc accggcgaga tagatcgcgg ccaaccatgc ccgccagctc
     3901 cacgaaggcg acggcgccgg cgtgagcgct acggttccgg ccgttgcagg ctctgtcgca
     3961 gtaactgccg cggcgaagga attcgataaa ggcgcgaggg cctcgggcgc gaccggcgcg
     4021 gccgagtgcg ccggcggtaa gacgcgcagc gaaacgtcgg gcgcccaggc gatcgcgatg
     4081 ggcaacgcga gcattgcgat catcaccacc gtccagacgc gatgccgcac cgcggccgcg
     4141 cgcacgcgca atgcccagag cacggctgcg gcgacggcgg cgatgaccgc cgcgcgcagc
     4201 gccgcttcga gcaagacatc gaacatcatg gggactccct ccggcgacgc gaccgcacgt
     4261 acttctccag ccgctcgaac tcctcgtcgc tcagcatctt ggcgtcgacc atgccgctga
     4321 cgagctcttc cacggagcct tggcagaagc ggtcgatgat ctggcgcacc gcgcgcgccg
     4381 cgagatgccc ggccggctcc gcggcgcgat agaggtaggc tcggccctcg gcctcgtgct
     4441 cgaggtagcc cttgtgctcg agccggcgca gcagcgtgcg cacggtggtt tctttgaggt
     4501 cgtacttgcg cgcgacgatg cggtgcacgt cctcgaccga gctgcgcgtg ccgctccaga
     4561 cggccttcat gacctcgctt tcgagctcgg tgagcggggc gcggacgcgc gcctttttgc
     4621 tacgttttgt catggtacac tgtgtaccac atcgcggtgc aggtcggcaa ggtgccccgc
     4681 ccggcgcgtt gtttgcgatc gcgacggctc gcgcggcgga gcgctcggcc taagcggccg
     4741 aggcgcgtaa tcggagacgc agcgctttcg cgactcg
//
LOCUS       DASSNN010000304         3278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_291384, whole genome shotgun sequence.
ACCESSION   DASSNN010000304 DASSNN010000000
VERSION     DASSNN010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291384"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VFL84_05550"
     CDS             complement(<1..1142)
                     /locus_tag="VFL84_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HET7608120.1"
                     /translation="MPTTITGSLPRPSWYTENLGLKAFLEAMVETRFREQYEDTLAVY
                     LNEQTIAGLDIVTDGDCRFTQDIGGQSWTSYPPFHMSGFDGVPKMAPVGGGGIGFPRG
                     HILHDYIEARMMPSIVGPVGRGNMQYAQIYKAAQRMTNKPVKFGTVTPELVAFAVQDF
                     HYKDIRERIWALSNAFNEELHALADAGCPVIQMEEPQIHLLAARGVVDKVINPEFMLE
                     VFNNTVKGLRAKTEVWCHTCWGNPSQQRMFAQVQSYKPALKLLNKVDADVITFESCSS
                     GGMDLEAIGKEITDMKVAIGVIDHHSLQVETPTEVADHIRKALKHIPVERLVLSSDCG
                     MGREGMARRHALYKMVSLVQGTNIIRKEHGIPEAECIAANADLTLL"
     gene            1368..2570
                     /gene="eboE"
                     /locus_tag="VFL84_05555"
     CDS             1368..2570
                     /gene="eboE"
                     /locus_tag="VFL84_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HET7608121.1"
                     /translation="MDLGNGLGHLTYSTLVHPGDTWVEMWDSLNRYVPQVKARVSPKK
                     SFGVSLRLSASSAQTLTADRGERERLKDFLKQHDMYLYTVNAFPYGPFKNRIVKKDVY
                     EPDWSTTEREVYTQQVADILADVAAPHVNPSIQSPPLGFKPKVTGPAYVNEFTRHVIN
                     TVAHMVLLEKRTGRTVTLCLEPEPACFLETTDEAVDYFTNHLYASEAVRMLVTATGLN
                     KSQAEAALHKHLGMVYDICHQAVEYEDIGASLRKLADNAIPVFKLQEAAALRVPNVTK
                     ETVAAMRPFAETVYLTQTIEKIDGKLNRYLNLEDAFAAFEANPSGKREWRTHVHVPVF
                     LDKIGQHFETTRFAIEDALKFHKKNKLSPQLEIETYTWDVLPDSMKTGSIVDYVEREL
                     DWVKGQLV"
     gene            2600..>3278
                     /locus_tag="VFL84_05560"
     CDS             2600..>3278
                     /locus_tag="VFL84_05560"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7608122.1"
                     /translation="MKVGFVGLGRMGQAMAGRILSGGHDLVVFNRSREKAAELEKNGA
                     KVAASIAEACRGREVVISMVSDDKALMEVVKEISGALEKGAIHVAMGTHGVHALREVD
                     AVHGKLGQKFVASPVLGRPEAAAAGQINIVPAGPADAIEKVAPLNALMGRRTFAAGSK
                     PEGAAAVKLANNMLIGCAIEAMGEAFSLVRKYGVDPAAFYEVFTDGLFAAPAYKVYGN
                     IIVKQLYG"
BASE COUNT          623 a         1021 c         1064 g          570 t
ORIGIN      
        1 tcgagcagcg tgagatccgc attcgccgcg atgcactcgg cctcggggat gccgtgctcc
       61 ttgcggatga tgttcgtgcc ctgcacgagc gacaccatct tgtagagcgc gtgccggcgc
      121 gccatgccct cgcggcccat gccgcagtcg gacgacagca ccaggcgctc gaccggaatg
      181 tgcttcagcg ccttgcggat gtgatccgcg acttccgtcg gggtctcgac ctgcaacgag
      241 tggtggtcga tgacgccgat cgcgaccttc atgtcggtga tctctttgcc gatcgcctcg
      301 aggtccatgc cgcccgagct gcacgactcg aacgtgatga cgtccgcgtc gaccttgttc
      361 agcagcttca gcgcgggctt atagctttgc acctgcgcga acatgcgctg ctgcgacgga
      421 ttgccccagc acgtgtggca ccagacttcc gtcttcgcgc gcaggccctt cacggtgttg
      481 ttgaacacct cgagcatgaa ctcggggttg atgaccttgt cgacgacgcc gcgcgccgcg
      541 agcaggtgga tctgcggctc ctccatttga atgacggggc agcccgcgtc ggcgagcgcg
      601 tgcagctctt cgttgaacgc gttgctcaag gcccagatgc gctcgcggat gtccttgtag
      661 tggaagtcct gcacggcgaa cgcgacgagc tcgggagtga cggtgccgaa cttgacaggc
      721 ttgttcgtca tgcgctgcgc ggctttgtag atctgcgcgt actgcatgtt gccgcggccg
      781 acgggcccga cgatgctcgg catcatccgg gcttcgatgt agtcgtggag aatgtggccg
      841 cgagggaagc cgatgccgcc gccgccgacg ggcgccatct tcggcacgcc gtcgaagccg
      901 gacatgtgaa acggcggata gctggtccag ctctggccgc cgatgtcttg cgtgaagcgg
      961 cagtcgccgt cggtgacgat gtcgaggccg gcgatcgttt gctcgttcaa atagacggcg
     1021 agcgtgtcct cgtattgctc gcggaatcgc gtctcgacca tggcttcgag gaacgccttg
     1081 aggccgagat tctcggtgta ccagctcggg cgcggcagcg agcccgtgat agtcgtaggc
     1141 aatagttggt ttgccgtcgc acgtagcatc agaaataccc cgtcaggttt tgaagcggcc
     1201 cgctgggagc cgacttattc gtgaaagccg gatacaatat tggagaaacg gcttccccac
     1261 aaccctaaac ggatcgagca atcccttacg atcagctcga cgtcgcggcg gcggaatcga
     1321 cggacgaccg ctcgcgcgga atcaaaagat ttaacgagga gcacgacatg gatttgggca
     1381 atggcctggg tcatctgact tattcgacgc tcgttcatcc gggcgacacc tgggtcgaaa
     1441 tgtgggacag cttgaaccgt tatgttccac aagtgaaagc acgtgtctcg ccgaagaaat
     1501 ctttcggcgt gtcgctgcgt ctctcggcgt cgtcggcaca gacgctgacg gccgatcgcg
     1561 gcgagcgcga gcggctcaag gacttcttga agcagcacga catgtacctc tacacggtga
     1621 acgcgttccc gtacgggccg ttcaagaacc gcatcgtcaa gaaggacgtc tacgagccgg
     1681 actggagcac gaccgagcgc gaggtgtaca cacagcaagt ggccgacatc ctcgccgacg
     1741 tcgcggcgcc gcacgtgaac ccgtcgatcc agagcccgcc gctcggcttc aagccgaagg
     1801 tgacgggccc ggcctacgtc aacgagttca cgcgccacgt catcaacacc gtcgcgcaca
     1861 tggtgctgct cgagaagcgc accggccgca ccgtgacgct gtgcctcgag cccgagcccg
     1921 catgcttcct cgagaccacc gacgaggccg tcgactactt cacgaatcac ctctacgcga
     1981 gcgaagcggt tcgcatgctc gtcacggcca cgggtctgaa caagtcgcag gccgaagccg
     2041 cgctgcacaa gcacttgggc atggtctacg acatctgcca ccaagcggtc gagtacgaag
     2101 acatcggcgc gtcgttgcgc aagctcgccg acaacgccat cccggtgttc aagctgcaag
     2161 aggccgcggc gctgcgcgtg ccgaacgtca cgaaggagac cgtcgccgcg atgcgcccgt
     2221 tcgccgagac cgtctatctc acgcagacga tcgagaagat cgacggcaag ctgaaccgct
     2281 atctgaacct cgaggacgcg ttcgcggcgt tcgaggcgaa tccaagcggc aagcgcgagt
     2341 ggcgcacgca cgtgcacgtg cccgtgttcc tcgacaagat cggccagcac ttcgagacga
     2401 cgcgcttcgc gatcgaggac gcgctgaagt tccacaagaa gaacaagctg tcgccgcagc
     2461 tcgagatcga gacgtacacg tgggacgtgc tgcccgattc gatgaagacg ggctcgatcg
     2521 tcgattacgt ggagcgcgag ctcgattggg tcaaaggcca gctcgtttaa ctggggccaa
     2581 ggggcagggg actaacgcaa tgaaagtcgg tttcgtcggc ctcggccgaa tgggtcaagc
     2641 gatggcgggc cgcatcttaa gcggcggtca cgatctcgtg gtgttcaacc gctcgcgcga
     2701 gaaagccgcc gagctcgaga agaacggcgc gaaagtcgcc gcgtcgatcg ccgaagcgtg
     2761 ccgcggccgc gaggtcgtga tcagcatggt gtccgacgac aaggcgctga tggaagtcgt
     2821 caaggagatc tcgggcgcgc tcgagaaggg cgcgatccac gtcgcgatgg gcacgcacgg
     2881 cgtgcacgcg ctgcgcgagg tcgacgccgt gcacggcaag ctcggccaga agttcgtcgc
     2941 atcgcccgtg ctaggccgcc cggaggccgc cgcagccggg cagatcaaca tcgtcccggc
     3001 cggaccggcg gacgcgatcg aaaaagtcgc gccgctgaac gcgctgatgg gtcgccgcac
     3061 gttcgcagcc ggctcgaagc cggaaggcgc cgcggccgtg aagctcgcga acaacatgct
     3121 gatcggctgc gcgatcgaag cgatgggcga ggcgttctcg ctcgtgcgca agtacggcgt
     3181 cgacccggcg gcgttctatg aggttttcac cgacggattg ttcgcggcgc ccgcttacaa
     3241 ggtctacggc aacatcatcg tgaaacagct ctacggcc
//
LOCUS       DASSNN010000305         4793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1068861, whole genome shotgun sequence.
ACCESSION   DASSNN010000305 DASSNN010000000
VERSION     DASSNN010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068861"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..954)
                     /locus_tag="VFL84_05565"
     CDS             complement(181..954)
                     /locus_tag="VFL84_05565"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7608123.1"
                     /translation="MTGRILAFLYGAIAYAVFFATFLYAVGFVGNFGVPKSMDSPAEG
                     PWQTALLIDAALLALFALQHSIMARPAFKRVLTRVVSPVVERSTYVLASSLALIVLFY
                     YWRPLGGIVWSVENPIGVALLYAGFAFGWGLVLVTTFVINHFDLFGLRQVWRHLNGQP
                     QQGLKFVTPLLYRTVRHPLYVGWFFAFWCTPTMTVTHLFFAIMTTAYILIAIQLEERD
                     LMREHAEYAAYRATVPMLIPFLRRRGRSEPPGVGAATQH"
     gene            1110..2015
                     /locus_tag="VFL84_05570"
     CDS             1110..2015
                     /locus_tag="VFL84_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET7608124.1"
                     /translation="MNRREALAMLAAATGTMAAPAYAQQLTASAPAAQPKIARKGRIK
                     QGLWRVNFGANTTLSFDDMCREAARLGAYGFDLIDPADWPTLRKHGLEPLLAGAGPVT
                     FQNGIIHPEVHDQLEKDLRPHIDMVASQGVKTIITIGGQRRGMPYEQAADNAVAFLNR
                     IKGHLEQRNVTLAIENMNNRRTDPNFGREDQVFGRWAWGVGVCERVNSPNVKLVCDLY
                     HLQIMDGDIAQNIRDTIQWIAHFHTAGVPSRNEIDFSQEMNYRYIAEVIADTPFTGYV
                     SHEWRPTPGRDPLRSIAEALAIMDV"
     gene            2061..4223
                     /locus_tag="VFL84_05575"
     CDS             2061..4223
                     /locus_tag="VFL84_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET7608125.1"
                     /translation="MNSSSFARGNRAAWLAALAASFVWCSASAQTAKRPPDGFIGGTV
                     TSARGPEAGVWVIAETDELKTPFIKIVVTDDAGRYVLPQLPTATYNVWVRGYGLADSD
                     KVEGRPGDTALNLTAKIASSPAEAAKVYPGNYWLSLLRPPAKSEFPGTGDSGNGIPPA
                     MQSQAHWMFNLKSGCNFCHQLGNEITRTLGHMDHLGFKTPEEAWIYRTQLGVRGSSMA
                     GTFAQFGVQAGARVLADWTTRIANGELPPVPPRPRPGPERNVVVTLWDWGVDQSFMHD
                     EVSTDKNDPTVNGYGPVYAVSAGHGKLNVLDPIENDAYELTIPTREDARKVQSRFPPP
                     AMPSNFWGMQHLWGLENPADPHNPMMDRKGRVWSTSKIRNEEPAWCREGSSHKFAKFY
                     PLNFSNRQASFYDPATGEFTLIDTCFATHHLQFDNDADQTIYFNELLGPIVGWINTRE
                     YDRTGDEQATQGWCAQIVDSNGDGKITKPWNVPGQPADARRDTEVRHNLYSVIPSPID
                     NSVWGASEDTPGYIVRIDLGDNPPETCKTEVYKVPEPGIDPRGIDIDSNGIVWTALAA
                     SSHLASFDRSKCRVFNGPRVVEGEQCAAGWKLYLTDGPRFKGTDIPADFHYYNWVDQH
                     DIAGFGANTPIATGSNSDALLVLDPKSGEWTTLRVPYPLGFYHRGLDGRIDDPAAGWK
                     GRGLWANYGTHLIWHTEGGKGTVGKAVHFQIRPDPLAR"
     gene            complement(4234..>4793)
                     /locus_tag="VFL84_05580"
     CDS             complement(4234..>4793)
                     /locus_tag="VFL84_05580"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET7608126.1"
                     /translation="HVDWKGYQQLLAMRGESSVPRITYLEGLAELMSPSRYHEIDKTR
                     FARLLEAWSESAGVPLEGYGSWTLEDEKADRGAEADECYTVRRVVKSDEERPDIAIEV
                     VWTSGGIDKLEVYRKLGVREVWLYERGTLRFFALRREADDDIYREIARSELLPELPIE
                     LLLACMKAPDQTSAVRALRAALAGG"
BASE COUNT          913 a         1610 c         1539 g          731 t
ORIGIN      
        1 gtcttcgcac atccctgtgc tcagacgctc tcgggcgggc ctctcggcac atcaacgcct
       61 tttgaggaca acgcgtcgac cgctctatgg aaagcagcga accgccggcg cacccggagt
      121 cgccgaacct cgcaagccgc cgagacgccc ggaagcgccg aacccgaacg aatccgaccg
      181 ctagtgctga gttgctgcgc ccacgccggg tggttcggag cgaccgcgac ggcgaaggaa
      241 cgggatcagc atcggcacgg tcgcgcgata ggctgcgtac tcggcgtgct cgcgcatgag
      301 gtcgcgctcc tcgagctgga tcgcgatgag aatgtacgcc gtcgtcatga tcgcgaagaa
      361 gagatgcgtg accgtcatcg tcggcgtgca ccagaacgcg aagaaccagc ccacgtacag
      421 cggatgtcgc accgtgcgat acagcagcgg cgtcacgaac ttcagcccct gctgcggctg
      481 accgttcaga tgccgccaga cttgccgcag gccgaacagg tcgaagtggt tgatcacgaa
      541 cgtcgtcacg agcaccaagc cccagccgaa tgcgaagcct gcgtacagca gcgcgacacc
      601 gatcgggttc tcgacgctcc agacgatgcc gccgagcggc cgccagtaat agaacaacac
      661 gatcagcgcg aggctactcg cgagcacgta cgtgctgcgc tcgacgaccg gcgacacgac
      721 gcgcgtcagc acgcgtttga acgcaggacg cgccatgatg ctgtgctgca gcgcgaacag
      781 cgcaagcaaa gcggcgtcga tcagcagcgc cgtctgccat ggaccttcgg ccggcgagtc
      841 catcgacttc ggcacgccga aattgcccac gaacccgacg gcataaagga acgtcgcgaa
      901 gaacaccgcg taggcgatcg ctccatagag aaacgcaagt atgcgacccg tcatcggatg
      961 taccccccat ttcgatattg cggagctcaa cgtcgctccg tgcacgatac tgtgcgcgcg
     1021 gcggggcccc ggcgctacgc tcgcgtgtct gcgcttggcc gccggcaagc gtcgaagata
     1081 agatggaccc ggtccaaagg ggggtgctca tgaatagaag agaagcgctc gcgatgctcg
     1141 cggcggcgac gggcacgatg gcggcgccgg cctacgctca acagctcacg gcaagcgccc
     1201 ccgcggcgca gcccaagatc gcgcgcaaag gccgcatcaa gcaagggctc tggcgcgtca
     1261 acttcggcgc caacacgacg ctgtcgttcg acgacatgtg ccgcgaggcc gcacggctcg
     1321 gtgcctacgg cttcgacctc atcgatcccg ccgactggcc gacgctgcgc aagcacggcc
     1381 tcgagccgtt gctcgccggt gcgggccccg tgacttttca gaacggcatc atccatccgg
     1441 aggtccacga ccagctcgag aaggatctgc gccctcacat cgacatggtc gcgagccaag
     1501 gcgtgaagac gatcatcacg atcggcggcc agcgccgcgg catgccgtac gagcaggcgg
     1561 ccgacaacgc ggtggctttc ttgaatcgca tcaaaggcca cctcgagcaa cgcaacgtca
     1621 cgctcgcgat cgagaacatg aacaaccggc gcaccgatcc gaacttcggc cgcgaggatc
     1681 aggttttcgg ccgatgggcg tggggtgtcg gcgtctgcga gcgcgtcaac tcgccgaacg
     1741 tcaagctcgt ctgcgatctc tatcacttgc agatcatgga cggcgacatc gcgcagaaca
     1801 tccgcgacac gatccaatgg atcgcgcact tccataccgc aggcgtgccg tcgcgcaacg
     1861 agatcgactt cagtcaggag atgaactacc gctacatcgc ggaggtcatc gccgacacgc
     1921 ctttcacggg ctacgtcagt cacgaatggc ggccgacgcc gggccgcgat ccgctgcgca
     1981 gcatcgccga ggcgcttgcg atcatggacg tatagaacgt ttcccaaagg gctcaaattg
     2041 ctttcattca ggggacttgc atgaacagct ctagctttgc ccgcggtaac cgggccgcgt
     2101 ggctcgcggc tctcgccgca tccttcgtgt ggtgcagcgc gagcgcgcag accgccaagc
     2161 ggccgcccga cggcttcatc ggcggcaccg tcacgagcgc gcgcgggccg gaggccggag
     2221 tgtgggtcat cgccgagacg gacgagctca agactccgtt catcaagatc gtcgtgaccg
     2281 acgacgccgg ccgctacgtg ctgccgcaac tgccgacggc gacgtataac gtctgggtgc
     2341 gcggctacgg gctcgcggac tccgacaagg tcgaagggcg ccccggcgat acggcgctga
     2401 atctcaccgc gaagatcgcg agctcgccgg ccgaggccgc caaggtctat cccggcaact
     2461 attggttgtc gttgctgcgg ccgccggcca agagcgaatt cccgggcacg ggcgacagcg
     2521 gcaacggcat cccgccggcc atgcagagcc aggcgcactg gatgttcaac ttgaagagcg
     2581 gctgcaactt ctgccatcag ctcggcaacg agatcacgcg cacgctcggt cacatggacc
     2641 acctgggctt caagacgcca gaggaagcgt ggatctatcg cacgcagctc ggcgtgcgtg
     2701 gcagctcgat ggccggcacg ttcgcgcagt tcggtgtgca ggccggcgcg cgcgtgctcg
     2761 ccgactggac tacacgcatc gcgaacggcg agctgccgcc ggtgccgccg cggccgcggc
     2821 cggggcccga gcgcaacgtc gtcgtgacgc tctgggattg gggcgtcgat cagtcgttca
     2881 tgcacgacga ggtttcgacc gacaagaacg atccgaccgt caacggctac ggccccgtgt
     2941 acgcggtctc cgcgggacac ggcaagctca acgtgctcga tccgatcgag aacgacgcct
     3001 acgagctcac gatcccaacg cgcgaggacg cgcgcaaggt gcagtcgcgt tttccgccgc
     3061 cggcaatgcc gtcgaacttc tggggcatgc agcatctgtg gggcctcgag aatccggccg
     3121 atccgcacaa tccgatgatg gatcgcaaag ggcgcgtctg gtcgacgtcg aagatccgca
     3181 acgaggagcc tgcgtggtgc cgcgaaggct cgagccacaa gttcgcgaag ttctatccgc
     3241 tgaatttcag caaccggcaa gcgtcgttct acgacccggc gacgggcgag ttcacgctga
     3301 tcgacacgtg tttcgcgacg catcacctgc agttcgataa cgacgcggat cagacgatct
     3361 acttcaacga gctgctcggg ccgatcgtcg gctggatcaa cacgcgcgag tacgatcgca
     3421 ccggcgacga gcaggcgacg cagggctggt gcgcgcagat cgtcgacagc aacggcgacg
     3481 gcaagatcac gaagccgtgg aacgtgccgg gccaacccgc cgatgcgcgg cgcgataccg
     3541 aggttcgcca caatctctat tccgtgatcc cgagcccgat cgacaactcg gtgtggggcg
     3601 cgtcggagga cacgcccggc tacatcgtgc gcatcgacct cggcgacaat ccgcccgaga
     3661 cgtgcaagac cgaagtctac aaagtgcccg agcccggcat cgatccgcgc ggcatcgaca
     3721 tcgattcgaa cggcatcgtc tggacggcgc tcgcggcgag cagccacctc gcgagcttcg
     3781 atcgctcgaa gtgccgcgtc ttcaacggcc cgcgcgtcgt agaaggcgag cagtgcgcgg
     3841 ccggctggaa gctttacttg accgacggcc cgcggttcaa gggcaccgac atcccggccg
     3901 acttccacta ttacaactgg gtggatcagc acgacatcgc gggcttcggc gcgaacacgc
     3961 cgatcgcgac gggcagcaac tccgacgcgt tgctcgttct cgacccaaag tcaggcgagt
     4021 ggacgacgct gcgtgtgccg tacccgctcg gcttctacca tcgcgggctc gacggccgca
     4081 tcgacgatcc ggccgccggc tggaaggggc gagggctctg ggccaactac ggcacgcact
     4141 tgatctggca caccgaaggc ggcaagggca ccgtcggcaa ggccgtgcat ttccagatca
     4201 ggcccgatcc gctcgcgcgc tgaccgcgcg gcgtcagccg ccggcgagcg ctgcgcgcag
     4261 cgcgcgcacg gccgacgttt gatcgggcgc cttcatgcac gcgagcaaca actcgatcgg
     4321 tagctcgggc aacagctcgc tgcgcgcgat ctcgcggtag atgtcgtcgt cggcttcgcg
     4381 ccgcaacgcg aagaagcgca gcgtgccgcg ctcgtagagc cagacttcgc gcacgccgag
     4441 cttgcgatag acctcgagct tgtcgatgcc gcccgaggtc cagacgacct cgatcgcgat
     4501 gtccggccgt tcctcgtcgc tcttgacgac gcggcgcacc gtgtagcact cgtcggcctc
     4561 ggcgccgcgg tccgcctttt cgtcctcgag cgtccacgag ccgtagcctt cgagcggcac
     4621 gcctgcgctc tcggaccacg cctcgagcaa gcgcgcgaag cgtgtcttgt cgatttcgtg
     4681 gtagcgcgac ggcgacatga gctcggccag gccctcgagg taggtaatgc gcggcaccga
     4741 gctctcgcca cgcatggcga gcagctgctg gtagcctttc cagtcgacgt gca
//
LOCUS       DASSNN010000306         3889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1188087, whole genome shotgun sequence.
ACCESSION   DASSNN010000306 DASSNN010000000
VERSION     DASSNN010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188087"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1173
                     /locus_tag="VFL84_05585"
     CDS             136..1173
                     /locus_tag="VFL84_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HET7608127.1"
                     /translation="MRADRLISIVLLLQANGRMTAETLASRLEVSQRTILRDMDALSS
                     AGVPVVAERGTGGGWRLIDGYETKLTGLTPAEIRSLFLARPPALLAELGIKEAADAAW
                     LKLRAALPVGVREQAEFVRQRLLIDSRNWRDSGESLSSLPVLLEALWGGQRLKFVYEK
                     ADGSSSEREVDPLGLVARANRWYLVAGKGDEQRTYRVSRIRSPEISTERAERPANFDL
                     AAYWEASTNRFREHLPYYNATFLVTEHILPWVCYRPWRVLEQAPAGERHRVSLRFDAP
                     EEARQFALAHGAELEVLEPQELREYVIEAARAVVAAYAGSAPPRPAADAVSCSLAVPA
                     TSARGSGPRGP"
     gene            complement(1112..1774)
                     /locus_tag="VFL84_05590"
     CDS             complement(1112..1774)
                     /locus_tag="VFL84_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7608128.1"
                     /translation="MSLVRKLVQRRGRPPHDDVLTPGEWAVVHFVRHGLSDARIAALR
                     HISRDAVKFHVRNALAKLGLRRRAELKRWVGVPKDSAAFGRTKTKEKSMTTELRLGPI
                     GQISRTVRDIDEACEWYGKVLGLKHLYTFGKLAFFDLGGTRLYLSAEGEAAGPESILY
                     LRVDDIGAAYSALVARGVEFNGAPHLIYKHADGTEEWMAFFKDPEGRFLALMSQVRPK
                     SS"
     gene            1832..3352
                     /locus_tag="VFL84_05595"
     CDS             1832..3352
                     /locus_tag="VFL84_05595"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET7608129.1"
                     /translation="MAIRRLLLATVTALALAGCGRKELGDADIVALDAVTLRAEIAAG
                     HVSALQVTQAYLRRIAAVDESGPMLGAVIEVNPDAEAIARALDEQRARGALGPLHGVP
                     VLIKANIDTADRMATSAGSLALAEHRARDDADVVTRLRAAGAVVLGKTNLSEWANFRS
                     TRSTSGWSSLGGQTRNPYVLDRNPCGSSSGSAVAVAAHLAPLAVGTETDGSIVCPAGV
                     NGVVGIKPAIGTVSQSGIVPIAHSQDTAGPLARTVADAALLLDVLAATGLPPAGPGSV
                     AGLRVGVVRNFGGAGSDPAVETTFTAALRRLEAAGLVLVDPVMAASPAELSNAERLIL
                     VSEFKEDLAAYLAGVRTGPRTFAALIEYNESHAADVMPYFGQELFLAAHGGLGTADPG
                     YAGALERVRQQRAALAALFRDRDLVALVAPANGRAWRTDYAGGDSGARVGSSRIAAIT
                     GFPSIAVPAALVDELPIGVAFVGQPQDEARLIAIAAAFERARGPFPEPKFLPMIAD"
     gene            complement(3359..>3889)
                     /locus_tag="VFL84_05600"
     CDS             complement(3359..>3889)
                     /locus_tag="VFL84_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HET7608130.1"
                     /translation="VLLHPGDNWMLHVAVELAKPGDIMVVAVSAPNSDGMFGDLLATS
                     FRARGVLGLVIDAGVRDVKDLTEMRFPVWSRAISAKGTVKATVGSVNIPIVCAGMLVN
                     PGDAIVADDDGVVVVPRRNAKTVLDASRKRVANEEEKRARLAAGELGLDIYKMREPLA
                     KAGLVYYDRQSDLDKS"
BASE COUNT          563 a         1364 c         1344 g          618 t
ORIGIN      
        1 gcgtcgcgat agagcagata cgcggtgatg gccatgggat cgtctccggg gttccgagct
       61 tgagccgcag atgatgccag gatagtcgcg cgttcatgac acgtgatgtc atgattgcgg
      121 tgtacggtac agcccatgcg cgcagaccgg ctcatctcca tcgttctact gctccaggcc
      181 aatggccgca tgacggccga gacgctggcg tcgcgtctcg aggtctcgca gcgcacgatc
      241 ttgcgcgaca tggacgcgct cagcagcgcc ggcgtgccgg tcgtcgccga gcgcggcacg
      301 ggcggcggct ggcgcctcat cgacggctac gagacgaagc tcacgggctt gacgccggcc
      361 gagatccgct cgctgttcct cgcgcgcccg cccgctttgc tcgcggagct cgggatcaag
      421 gaagcggccg acgccgcctg gctcaagctg cgcgccgcgc tgcccgtggg cgtgcgcgag
      481 caagccgaat tcgtgcggca gcgcttgctc atcgactcgc gcaactggcg cgactcgggc
      541 gagtcgctgt cgagcctgcc cgtgctgctc gaggcgctct ggggcggcca gcgcctcaag
      601 ttcgtttacg agaaggccga cggctcctcg agcgagcgcg aagtcgatcc gctcgggctc
      661 gtcgcgcgcg cgaaccgctg gtatctcgtc gcgggcaagg gcgacgagca acgcacgtat
      721 cgcgtgtcgc ggatccgctc gccggagatc tcgaccgagc gcgcggagcg tccggcaaac
      781 ttcgatctcg ccgcgtattg ggaagcctcg acgaatcgct ttcgcgagca cctgccctac
      841 tacaacgcga cgttcctcgt caccgagcac atccttccgt gggtctgcta tcggccgtgg
      901 cgcgtgctcg agcaagcacc ggccggcgag cgccatcgcg tgtcgctgcg tttcgacgct
      961 cccgaagaag ctcggcagtt cgcgctcgcg catggcgccg agctcgaggt gctcgagccg
     1021 caagagctgc gcgagtacgt gatcgaggcg gcccgcgccg tcgtcgcggc ctacgcggga
     1081 tcggcgccgc cgcgcccggc agccgacgca gtcagctgct ctttggccgt acctgcgaca
     1141 tcagcgcgag gaagcggccc tcggggtcct tgaagaacgc catccactcc tcggttccgt
     1201 cggcgtgctt gtagatcaga tgcggcgcgc cgttgaactc gacgccgcgc gcgacgagcg
     1261 cgctgtacgc cgcgccgatg tcgtcgacgc gcaaatacaa aatcgattcg ggccccgccg
     1321 cttcgccttc ggcgctcaaa tagaggcgcg tgccgccgag gtcgaagaac gcaagcttgc
     1381 cgaacgtata gaggtgcttg aggccgagca ccttgccgta ccattcgcac gcttcgtcga
     1441 tgtcgcgaac cgtgcgcgaa atctgcccga tcggacccag gcgcagctct gtcgtcatgg
     1501 acttctcctt ggtcttcgtg cggccaaacg cggcgctgtc cttcggcacg cccacccagc
     1561 gcttgagctc cgcgcggcgg cgcagcccga gcttcgcgag cgcgttgcgc acgtggaact
     1621 tcacggcgtc gcggctgatg tggcgcagcg cggcgatgcg cgcgtcgctc aatccgtgcc
     1681 gaacgaagtg cacgacggcc cattcgccgg gcgtcaacac gtcgtcgtgc ggcggacggc
     1741 cgcggcgctg aacgagcttg cgcaccaagg acatggcgct gatggtagcg ttccgctagc
     1801 gtcatttccc tccccctatc gggcgaacac catggcaata cgtcggctcc tcctcgcgac
     1861 cgtgaccgct ttggcgctcg cgggctgcgg ccgcaaggag ctcggcgacg ccgacatcgt
     1921 ggcgctcgac gccgttacgc tgcgtgcgga gatcgcggcc gggcacgtct cggcgttgca
     1981 ggtcacgcag gcgtacttgc gaaggatcgc ggccgtcgac gaatcgggcc cgatgctcgg
     2041 cgccgtcatc gaggtcaatc ccgatgccga agcgatcgcg cgcgccctcg acgagcagcg
     2101 cgctcgcggc gcgctcggcc cgctgcacgg cgtgccggtc ttgatcaagg cgaacatcga
     2161 tacggccgac cgaatggcga ccagcgccgg atcgctggct ctcgccgagc atcgcgcccg
     2221 cgacgatgcc gacgtcgtca cgaggctgcg cgccgcgggc gccgtcgtgc tcggcaagac
     2281 caacttgagc gagtgggcga acttccgctc gacgcgctcg acgagcggct ggagcagcct
     2341 cggcggccag acgcgcaatc cgtacgttct cgatcgcaat ccgtgcggct cgtcgagcgg
     2401 ctcggccgtc gccgtcgcgg cgcatctcgc gccgctcgcg gtcggcaccg agaccgacgg
     2461 ctcgatcgtc tgcccggccg gcgtcaacgg cgtcgtcggc atcaagcccg cgatcggcac
     2521 ggtcagccag agcggcatcg tgccgatcgc gcacagccag gacacggcgg ggccgttggc
     2581 gcgcacggtc gccgacgcgg cgctgctgct cgacgtgctc gccgcaacgg gcctgccgcc
     2641 cgcgggcccg ggcagcgtcg cgggcttgcg cgtcggcgtc gtcagaaact tcggcggcgc
     2701 aggaagcgat cccgcagtcg agacgacgtt cacggcggcg ctgcgccggc tcgaagcggc
     2761 cggtctcgtg ctcgtcgatc ccgtcatggc cgcatcgccc gccgagctct cgaacgcgga
     2821 gcggctgatc ctcgtgtccg agttcaagga ggacctcgcg gcgtatctcg ccggcgtgcg
     2881 gaccggcccg cgaacgtttg ccgcgctgat cgagtacaac gagtcccacg cggccgacgt
     2941 catgccgtat ttcgggcaag agctgttctt ggccgcacac ggagggctcg gcacggccga
     3001 tccgggctac gccggcgcgc tcgagcgcgt aaggcagcag cgtgccgcgc tcgccgcgct
     3061 gttccgcgac cgcgatctcg tcgcgctcgt cgcgccggcg aacgggcgcg cctggcgcac
     3121 ggattacgcg ggcggcgaca gcggggcgcg cgtcggcagc tcgcgcatcg cggcgatcac
     3181 gggctttccg agcatcgcgg tgcccgccgc gctcgtcgac gaattgccga tcggcgtcgc
     3241 gttcgtcggg caaccgcaag acgaggcgcg gctcattgcg atcgcggccg cgttcgagcg
     3301 cgcgcgcgga ccgttcccgg agccgaaatt cctgccgatg atcgcggatt gagcgccgtc
     3361 agctcttgtc gaggtcgctc tgccggtcgt aatagacgag acccgctttc gcgagcggct
     3421 cgcgcatctt gtagatgtcg agcccgagct cgccggcggc gagcctcgcg cgcttttcct
     3481 cttcgttcgc gacgcgcttt ctgctcgcgt cgaggaccgt ctttgcgttg cggcgcggca
     3541 cgaccacgac gccgtcgtcg tcggcgacga tcgcgtcgcc ggggttcacg agcatgcccg
     3601 cgcacacgat cggaatgttg acggagccga ccgttgcctt gacggtgccc ttcgccgaga
     3661 tcgcgcggct ccacaccggg aagcgcatct ccgtgaggtc cttgacgtcg cgcacgccgg
     3721 catcgatgac gagcccgagc acgccgcgcg cgcggaacga cgtcgcgagc aggtcgccga
     3781 acatgccgtc ggagttcggc gcgctaacgg cgacgaccat gatgtcgccg ggcttcgcga
     3841 gctcgacggc gacgtgcagc atccagttgt cgccgggatg cagcagcac
//
LOCUS       DASSNN010000307         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1441063, whole genome shotgun sequence.
ACCESSION   DASSNN010000307 DASSNN010000000
VERSION     DASSNN010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441063"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..610
                     /locus_tag="VFL84_05605"
     CDS             191..610
                     /locus_tag="VFL84_05605"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7608131.1"
                     /translation="MKRASILTVAAAGALLSTASASAHHSFAAEFDRALPITVTGTVT
                     KVEWMNPHARFYIDAKDDQGKIVNWDFELASPNGLMRRGWNRNSMKIGDTVTVAGHRA
                     KNNPHVGNASSVVLANGKKLFAGSSIENEPAAPAQSQ"
     gene            983..1642
                     /locus_tag="VFL84_05610"
     CDS             983..1642
                     /locus_tag="VFL84_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7608132.1"
                     /translation="MRDRTAIENFLALGADPNETEPDGTTPLMRAVHGRMPNTAQQLI
                     DAGADVKKANSYGVTALYVAARGGDSAATRMLLAAGADANAALPASGETVLMTAAKAG
                     DADVVRALLTGGDDNVSLAALGAARAAARVAEGAGYETPANPALDTNYADVNARDRLY
                     GRTALIIAAIEGDLEIVRMLIEAGSDVNVVDAEGATALSLARSYGNLDVAALLEEAGA
                     N"
     gene            1768..2196
                     /locus_tag="VFL84_05615"
     CDS             1768..2196
                     /locus_tag="VFL84_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7608133.1"
                     /translation="MDKTYKGSCFCGAVSLLVTGEPVTMGYCHCQSCRHWSAGPVNAY
                     TLWRPGAVKITAGAENIGSYSKTPASVRKWCKACGGHLLTEHPAIKLIDVYAAVLPGL
                     EFKPREHLHYQEAVLRIVDSLPKHKDLPKSAGGSGVLMPS"
     gene            complement(2282..>2546)
                     /locus_tag="VFL84_05620"
     CDS             complement(2282..>2546)
                     /locus_tag="VFL84_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET7608134.1"
                     /translation="GAINGVLWGVVMAAATWLLYGDPGLAAVMAAAMVLNILLASAVG
                     VLCPMILQRMGRDPVMGSSILLTAITDSMGFFIFLALASLLLL"
BASE COUNT          494 a          875 c          799 g          378 t
ORIGIN      
        1 ctggcgtcgt cccgactacg gccatctcga gatcgacgtc accgtcgacg atccgaaggc
       61 gtacacgaag ccgttcacgg tccgcgtgag ccagcagctc accgtcgatc aggagctgat
      121 cgagttcatc tgcaacgaga acgagaagtc ctcgcagcac tacgatccat agattccgaa
      181 ggagcactga atgaaacgcg cttcgatact gacggtcgcc gctgcgggcg cactgctctc
      241 gacggcctca gcctcggccc atcactcgtt cgccgcggag ttcgaccgcg cgctgccgat
      301 caccgtgacg ggcacggtca cgaaggtcga atggatgaac ccgcacgcac ggttctacat
      361 cgacgcgaaa gacgaccagg gcaagatcgt gaactgggac ttcgagctcg cgagcccgaa
      421 cgggctcatg cgccgcggct ggaaccgcaa ttcgatgaag atcggcgaca ccgtcacggt
      481 cgcgggccac cgcgcgaaga acaatccgca cgtcggcaac gcgagctccg tcgtgctcgc
      541 caatggcaag aagctgttcg caggctcctc gatcgagaac gagccggcgg cgcccgcaca
      601 atcccagtga cgcaggccga tataaccatc gaagatccaa gggcttacac gaaacggttt
      661 acggttcacg tgaagcaggg gccgttccac ggcgccgagc tcatgggtct tggccgcaat
      721 gaaaatgaga agtcggtccg ttgcgacgac ccccgagcgg taaacgctga agctccgttc
      781 aggtttcccg cgtatcattg gcactgccaa aggctgcctc cggtggccaa tcggggaacg
      841 aatgcgggtg cggctgtcgt cgtactttaa gggttgggct ttgatcgcag ccgccacgct
      901 ggcaggctgc ggcactgccc cgccgccccc ggccaccggc agaggccaat accagcccgc
      961 cacgctcgca gaagccgtca tcatgcggga ccggacggcg atcgagaact tcctcgccct
     1021 cggcgccgat cccaacgaga cggagccgga cggcacgaca ccgttgatgc gggccgtgca
     1081 cggccggatg ccgaacacgg cgcagcagct catcgatgcc ggcgccgacg tcaagaaagc
     1141 gaactcctac ggcgtgacgg cgctctacgt cgccgcgcgc ggcggcgaca gcgccgcgac
     1201 gcgcatgctg ctcgccgccg gcgccgacgc caacgcggcg ctgccggcgt ccggcgaaac
     1261 cgttctcatg acggccgcga aggccggcga tgccgacgtc gtgcgcgcgc tcttgaccgg
     1321 cggcgacgac aacgtctcgc tcgcggcgct cggcgccgcg cgcgcggcgg cgcgcgtcgc
     1381 cgagggcgcc ggctacgaga cgccggcgaa cccggctctc gacacgaact acgccgacgt
     1441 caacgcgcgc gaccgactgt acgggcgcac ggcgttgatc atcgcggcca tcgaaggcga
     1501 tctcgagatc gtgcgcatgc tgatcgaagc cggctccgac gtgaacgtcg tcgacgccga
     1561 aggcgcgacg gcgttgtcgc tcgcgcgcag ctacggcaat ctcgacgtcg ccgcgctgct
     1621 cgaggaagcc ggcgcaaact agaccgcgcc tccactccgg cgcgcggcgc ccgctaattg
     1681 cgctcgcgcg gggcaaacct ttccgcgagc gtcagctata gtagcggccc ggccgaatcc
     1741 aagaatttcg agctgatcgg ggaacccatg gacaagacct acaagggcag ctgcttttgc
     1801 ggcgccgtct cgctgctcgt gaccggcgag cccgtcacga tgggctattg ccattgccaa
     1861 tcgtgccgcc actggtccgc gggtcccgtc aacgcgtaca cgctctggcg ccccggcgcc
     1921 gtcaagatca cggccggcgc cgagaacatc ggcagctata gcaagacgcc ggcgagcgtg
     1981 cgcaagtggt gcaaagcctg cggcggccat ctgctgaccg agcacccggc gatcaagctc
     2041 atcgacgtct acgcggccgt gctgcccggc ctcgagttca agccgcgcga gcacctgcac
     2101 tatcaggagg ccgtgctacg catcgtcgac tcgctgccga agcacaagga cctgccgaag
     2161 tccgccggcg gctccggcgt gctgatgccg tcgtaaacga aaatcgtaaa aggggacaga
     2221 tctatttttc cttctttaaa tagatctgtc cccttttaac ggtccccttt taacgggcgg
     2281 cctataacaa gagcagcgac gctagcgcga ggaagatgaa gaaccccatg ctgtcggtga
     2341 tggccgtcag caggatgctg ctgcccatga cgggatcgcg gcccattcgc tgcaagatca
     2401 ttgggcacaa cacgccgacc gccgacgcca acaggatgtt caacaccatc gcagccgcca
     2461 tcacggccgc caagcccggg tcgccgtaga gcagccacgt cgccgccgcc atcacgacgc
     2521 cccacaggac gccgttgatg gctccg
//
LOCUS       DASSNN010000308         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_187211, whole genome shotgun sequence.
ACCESSION   DASSNN010000308 DASSNN010000000
VERSION     DASSNN010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187211"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1868)
                     /locus_tag="VFL84_05625"
     CDS             complement(12..1868)
                     /locus_tag="VFL84_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04551 family protein"
                     /protein_id="HET7608135.1"
                     /translation="MPKNLVLPMRYISSSVVLGIALISEVASAQMGPGMGGGGMGGPA
                     QPAGEEKKEGVAEEAPKTPGLLPTTPALPPAKGRRKRWKLLELDGYFRLRTDWFKNFN
                     LGFNSDPALGGGPFPRALGCGVDRAAGGATNAPCNDSLASTNMRLRLEPTFNIDEGTS
                     VHVQADVLDNLVLGSTPTTGISPGFTTGLPPNGAFNTTQDPPVQGVNSDRDSIVIKRA
                     WAEVAVPLGILKFGRMPNHWGMGIYANGGGRDPINGTYDYDADYGDTVDRLSFSTTIP
                     GTSLRGMVATDWNSTRLVSNQTSANLGREGHPYDLDDSDDATSWIGVISHMDTPQEFR
                     DAVDRGERVVNYGVYFEYKTQSWDDNTTGFTPGGTFDATAHYVPRDAKTYTASLWGKF
                     GTGAITLEGELVGQFGSIKHLDDFGFTRTADIRKAGGAARMIWRGDEGKLRLGIEGGF
                     ATGDQWDNTPQGNTNIAFANRLGDPALCNASSCTLTQFMFNREYKIDLIMWRHLMGAV
                     TNAAYAKPFLQYDLTKSIMFRASNVTSFALKSVATPGNGTMYGTEFDGDLGYNGSRFF
                     AGVSYGVLFPFGAMAHPQDSELTAGPGFGYGAGNVGDPSTAHTIQMRLVLAF"
BASE COUNT          329 a          725 c          777 g          370 t
ORIGIN      
        1 tcagcaacct gctagaacgc cagcacgagc cgcatctgga tcgtgtgcgc cgtcgacgga
       61 tcgccgacgt tgccggcgcc gtacccgaaa ccgggcccag ccgtcagctc gctgtcctgc
      121 gggtgcgcca tcgcgccgaa cgggaacagc acgccgtacg acacgccggc gaagaaccgc
      181 gagccgttgt agccgaggtc gccgtcgaac tccgtgccgt acatcgtgcc gttgccgggc
      241 gtcgcgaccg acttgagcgc gaacgacgtc acgttcgagg cgcggaacat gatcgacttc
      301 gtgaggtcgt actggaggaa cggcttggcg tacgcggcgt tcgtgacggc gcccatcagg
      361 tgccgccaca tgatgaggtc gatcttgtac tcgcggttga acatgaactg cgtcagcgtg
      421 cagctcgacg cgttgcacag cgcgggatca ccgagccggt tcgcgaacgc gatgttcgtg
      481 ttgccctgcg gggtgttgtc ccactgatcg cccgtcgcga acccgccctc gatgccgagg
      541 cgcagcttgc cctcgtcgcc gcgccagatc atgcgcgccg cgccgcccgc cttgcgaatg
      601 tcggcggtgc gcgtgaaccc gaagtcgtcg aggtgcttga tcgagccgaa ctgcccgacg
      661 agctcgccct ccagcgtgat cgcaccggtc ccgaacttgc cccacaggct ggccgtgtag
      721 gtcttggcgt cgcgcgggac gtagtgcgcg gtcgcgtcga acgtgccgcc cggcgtgaag
      781 ccggtcgtgt tgtcgtccca gctctgcgtc ttgtactcga agtacacgcc gtagttgacg
      841 acgcgctcgc cgcggtcgac cgcgtcgcgg aactcctgcg gcgtgtccat gtgcgagatc
      901 acgccgatcc agctcgtcgc gtcgtccgag tcgtcgagat cgtacggatg gccctcgcga
      961 ccgaggttcg cgctcgtctg gttcgacacg aggcgcgtcg agttccagtc cgtcgcgacc
     1021 atgccgcgca gcgaggtgcc cgggatcgtc gtcgagaagc tcagacggtc gacggtatcg
     1081 ccgtagtcgg cgtcgtagtc gtaggtgccg ttgatcggat cgcggccgcc gccgttggcg
     1141 tagatgccca tgccccagtg gttcggcata cggccgaact tgaggatgcc gagcggcacg
     1201 gccacctcgg cccacgcacg cttgatgacg atcgagtcgc gatcgctgtt gacgccctgg
     1261 acgggcgggt cctgcgtcgt gttgaacgcg ccgttcggcg gcagcccggt cgtgaagccg
     1321 ggcgagatcc ccgtcgtcgg cgtcgagccg agcacgaggt tatcgaggac atcggcctgg
     1381 acgtggaccg acgtgccctc gtcgatgttg aacgtgggct cgaggcggag ccgcatgttc
     1441 gtgctcgcga gcgagtcgtt gcacggcgcg ttcgtcgcac caccggccgc gcgatcgacg
     1501 ccgcagccga gcgcgcgcgg gaacgggccg ccgccgagcg cgggatcgct gttgaagccg
     1561 aggttgaagt tcttgaacca gtcggtgcgc agccggaagt agccgtcgag ctcgagcagc
     1621 ttccagcgct tgcggcggcc cttcgcgggc ggcagcgccg gcgtcgtcgg caagaggccc
     1681 ggcgtcttcg gcgcttcctc cgcgacgcct tccttcttct cctcgcccgc cggctgcgcg
     1741 gggccaccca tgccgccgcc gcccatgccg ggacccatct gcgccgaggc gacctcggag
     1801 atcagagcga tgcctaaaac gacgcttgat gagatgtaac gcatgggaag cacgaggttt
     1861 ttaggcattc gctgagggtc ggcggactat atgcacgagg tccggggcgg tcaatcggcg
     1921 cgggcgtcga tccgggcatc cgaccgcgaa ttcgccgggt ccagatcgga cgcgttatgt
     1981 tgacttcgat ggcactgctg cgaccgcgtg aagacagccc ggtccgccgc gcgaggcgcg
     2041 ccgagcgcga ggcgcgccga cgaagcgctc ccgggcggaa cgcgaggagg cgcaacgaag
     2101 cgattcggcg atggatcgcg cggctgacgg gctggggtca cgcggttgca gcagtagacc
     2161 gtggtcggcg cgccggcgtt gcctgggtat tgttcgcaac g
//
LOCUS       DASSNN010000309         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_561710, whole genome shotgun sequence.
ACCESSION   DASSNN010000309 DASSNN010000000
VERSION     DASSNN010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561710"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /gene="acnA"
                     /locus_tag="VFL84_05630"
     CDS             complement(<1..1497)
                     /gene="acnA"
                     /locus_tag="VFL84_05630"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HET7608136.1"
                     /translation="MTDSFGARKSLAVRSIDYQIFRLDAVKGAVDTLPYTLKILLENM
                     LRFEDGANITAEDITALANWDPKAEPDREISFTPARVVLQDFTGVPAVVDLAAMRDAV
                     VKLGGKAQRINPLSPAELVIDHSVQVDFFGTPDAIKKNTAIEFERNLERYAFLRWGQN
                     AFQNFRVVPPNTGIVHQVNLEYLSRVVFDKPVGKDRYAYPDTLVGTDSHTTMINGLGI
                     LGWGVGGIEAEAAMLGQPITMLIPQVVGFELKGKLAEGATATDLVLTVTEMLRKRGVV
                     GKFVEFFGEGLDHLPLADRATIGNMSPEFGSTCAVFPIDAETLRYLELSGRSREQIDL
                     VEAYAKTQGLWRSSKIARYSDVLTLDMGAVEPSISGPKRPQDRIALKGAGAKIGDLLK
                     TAGKGTKAPVSAGSKKFDLEDGAVVIAAITSCTNTSNPAVMVGAGLLARNAAAKGLTR
                     KPWVKTSLAPGSKVVSDYLAACKLDDDLDALGFNLVGYGCTTCVAEGTQ"
     gene            complement(1566..2684)
                     /gene="mnmA"
                     /locus_tag="VFL84_05635"
     CDS             complement(1566..2684)
                     /gene="mnmA"
                     /locus_tag="VFL84_05635"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET7608137.1"
                     /translation="MPSSPLTIVALSGGVDSAVAALLLRDAGHAVQCLHMSNWEDDGY
                     CDNAREFQDARRVCLELDLPLHRVNFAREYREQVFADFLAEYRAGRTPNPDVLCNREI
                     KFGVMRRYAQRLNATQIATGHYARVENVDGVPRLLKGVDPAKDQSYFLHAVAADDFVD
                     VLMPLGGLFKTEVREIARKAGLAVAEKKDSTGICFVGERPFAEFLSNYLPDNPGVIRD
                     DLGIERGRHRGLAYYTLGQRHGLNIGGASDRPENAWYVAAKDSARNELIVVQGHEHPL
                     LKSGSLAATNVHWIGAPPGAWERGEPLRCHAKIRYRQPDQACTVLRTAESSFEARFDE
                     PQRSPTPGQFIVLYDGDRCLGGATIEAAAVSAALRAAV"
BASE COUNT          435 a          878 c          930 g          471 t
ORIGIN      
        1 ctgcgtgccc tcggcgacgc atgtggtaca gccgtagcca acgaggttga aacccaacgc
       61 atcgaggtcg tcgtccagct tgcaagccgc gagatagtcg ctgacgactt tcgagcccgg
      121 cgccaagctc gtcttgaccc acggcttgcg cgtgaggccc ttcgccgccg cattgcgcgc
      181 gagcagcccc gcgccgacca tgacggccgg gttcgacgtg ttcgtgcagc tcgtgatcgc
      241 tgcgatcacg acggcgccgt cctcgaggtc gaatttcttc gaacccgccg agacgggcgc
      301 cttcgtgcct ttcccggcgg tcttgagcag atcgccgatc ttcgcgcccg cgcccttcag
      361 cgcgatgcga tcctgcggcc gcttcgggcc cgagatgctc ggctcgacgg cgcccatgtc
      421 tagcgtcaac acgtcgctat agcgcgcgat cttcgagctg cgccacaagc cttgcgtctt
      481 cgcgtaggcc tcgacgagat cgatctgctc gcgcgagcgg cccgagagct cgaggtagcg
      541 cagcgtctcg gcgtcgatcg gaaacacggc gcaggtgctg ccgaactccg gcgacatgtt
      601 gccgatcgtc gcgcggtccg cgagcggcag gtggtcgagg ccttcgccga agaactcgac
      661 gaacttgccg acgacgccgc gcttgcgcag catctcggtc accgtcagca cgagatccgt
      721 ggccgtcgcg ccttcggcga gcttgccttt gagctcgaag ccgacgacct gcggaatcag
      781 cattgtgatc ggctgcccga gcatcgcggc ttccgcctcg atgccgccga cgccccagcc
      841 caggatgccg aggccgttga tcatcgtcgt gtgcgagtcc gtgccgacga gcgtatccgg
      901 atacgcgtag cggtccttgc cgaccggctt gtcgaacacg acgcggctca gatactcgag
      961 gttgacctgg tgcacgatgc cggtgttcgg tggcacgacg cggaagttct ggaacgcgtt
     1021 ctgaccccag cgcaagaaag cgtaacgctc gaggttgcgc tcgaactcga tggccgtgtt
     1081 cttcttgatc gcgtcgggag tgccgaagaa gtcgacctgc accgagtgat cgatgacgag
     1141 ctcggcgggc gagagcgggt tgatgcgctg cgccttgccg ccgagcttga cgaccgcgtc
     1201 gcgcatcgcg gcgaggtcca cgacggccgg gacgcccgtg aagtcctgca gcacgactcg
     1261 cgccggcgtg aaagagatct cgcggtccgg ctcggccttc gggtcccaat tcgcgagcgc
     1321 ggtgatgtcc tcggccgtga tgttcgcgcc gtcctcgaag cgcagcatgt tctcgagcag
     1381 gatcttcagc gtgtagggca gcgtgtcgac ggcgcctttg acggcgtcga gcctaaagat
     1441 ctgatagtcg atggagcgga cggcgagcga cttgcgagcg ccgaaactgt cggtcattgg
     1501 aacctctgag gacgaaatcg attggaacga cgggattata agcgggaaac cttacgcggc
     1561 ggcgatcaga ccgcggcccg aagcgccgca gacacggccg cggcttcgat cgtcgcgccg
     1621 cccaagcagc ggtcgccgtc gtagagcacg atgaactgtc ccggcgtcgg cgagcgctgc
     1681 ggctcgtcga agcgagcttc gaaagagctc tcggccgtgc ggagcaccgt gcaggcctga
     1741 tcgggctggc ggtagcggat cttcgcgtgg cagcgcaacg gctcaccgcg ctcccatgcg
     1801 cctggcggcg cgccgatcca gtgcacgttc gtcgcggcga gcgagccgct cttgagcagc
     1861 gggtgctcgt ggccttgcac gacgatgagc tcgttgcgcg cgctgtcctt cgcggccacg
     1921 taccaggcat tctccggccg gtccgaggcg ccgccgatgt tgaggccgtg ccgctggccg
     1981 agcgtgtaat aggcgaggcc gcgatgcctg ccgcgctcga tgccgaggtc gtcgcgaatg
     2041 acacccgggt tgtcgggcag gtagttgctc aagaactccg cgaacggccg ttcgccgacg
     2101 aagcagatgc ccgtgctgtc cttcttctcg gcgacggcga ggccggcctt gcgcgcgatc
     2161 tcgcggacct cggtcttgaa caggccgccg agcggcatca gcacgtcgac gaagtcgtcg
     2221 gccgcgacgg cgtgcaggaa gtagctctgg tccttggccg ggtcgacgcc ttttagcagg
     2281 cgcggcacgc cgtcgacgtt ttcgacgcgc gcgtaatgac ccgttgcgat ttgcgtcgcg
     2341 ttgaggcgct gcgcgtagcg gcgcatgacg ccgaacttga tctcccggtt gcagagcacg
     2401 tcggggttcg gcgttcggcc ggcgcggtac tcggccagga agtccgcgaa cacttgctcg
     2461 cggtattcgc gcgcgaagtt gacgcggtgc agcggcagat cgagctcgag gcagacgcgg
     2521 cgcgcgtcct gaaactcgcg ggcattgtcg cagtagccgt cgtcttccca gttgctcatg
     2581 tgcaagcact ggacggcatg acccgcatcg cgcagcagca gcgcggcaac ggcggaatcg
     2641 acgccgcccg agagagcgac gatggtcaga gggctcgaag gcatgccggc attttagcag
     2701 ccgacaagaa gggt
//
LOCUS       DASSNN010000310         4398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_187652, whole genome shotgun sequence.
ACCESSION   DASSNN010000310 DASSNN010000000
VERSION     DASSNN010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187652"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..366
                     /locus_tag="VFL84_05640"
     CDS             166..366
                     /locus_tag="VFL84_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608138.1"
                     /translation="MKSEEAKPLQVAAQHAANWCMEKLEMKEGGFGKDHAHSDSERAA
                     LHVAHSIAICEIARLQSIVDGR"
     gene            complement(380..1597)
                     /locus_tag="VFL84_05645"
     CDS             complement(380..1597)
                     /locus_tag="VFL84_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010440260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7608139.1"
                     /translation="MSTPIADIAAVPDDGRAKRNVAVLVFAQAILGAQLATNIIVAGW
                     AGKVLAPDPSLATLPISIVVLGSLLTAPTMSLFMSRYGRRTGFWVATLAAAIASALCA
                     RALFVGSFALFLTGSVLLGVFQATQGFFRFAATDTASDAFKPKAISWVLAGGLLSALL
                     GPQIADFGADAIANVPYAGAYIAMIALNVVGAIGLKFLDIPIPPRAAKAAETGRPLAE
                     IARQPAFVVAVLAAMVGFSAMSLVMTSTPLAMVDHGLTHGNATDVVRWHIVAMFAPSF
                     FTGSLIVRFGYLPVIVAGLLLLGVCGAIAVAGVELHHFYLALIALGIGWNFSYVGATS
                     LLGTTHTRAEQAKVQGLNDFLVLGLVAVGSFGSGALLSTYGWTAVQYAMAPALLIALA
                     GIAWLALVARSTE"
     gene            1666..3384
                     /locus_tag="VFL84_05650"
     CDS             1666..3384
                     /locus_tag="VFL84_05650"
                     /inference="COORDINATES: protein motif:HMM:NF022164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaF N-terminal domain-containing protein"
                     /protein_id="HET7608140.1"
                     /translation="MQTIAYPKEQLHRISFTKLTEAQIASKLGPTPAPKSASPLSTAL
                     AGKSLRIVTDNGPSLRYRFTGNNRLSLVENDGRAVEADYGALTLDHVTLFSHLLQGTQ
                     RGYHVVVDHASGLATVFEVWFSDFTDSREVQREIYFGYVEEAGKEAPKARHSLTNRVE
                     GKGFHWTQDTGVETLELYPSTFYSNFVELSRQGGELGFCAPTDYVKIDDELYVYSRVE
                     CEFSGVQTLYVFDANRAEQVGVRLGFDANDTLEYYLFTGRGEWVGQLAQFEKFGDTAA
                     TPMPPPANGAPLAKGARRVYRPMRNNWQMTKEEVAALTEKNVFGAPSAMAGNKSPVSD
                     YLVGKELTLRFDNGVAADYRFDKIDTLHWRRPGESTWHEERYEAWESAPGVVMFGHLL
                     TGAPQKDCYKLVADFDHGLVTCIHGTIGSRYIANEAQAETWFGVVEMEGIVPPKYRRH
                     HFTDELVGRAITWNYSPGLTSMHLYTTPHSLSWIIFGESGAGGMEWSGPASYVKIRDE
                     LYLAYWLEEACNGTLGTILVNLRTMHDCGIGYHCGRDGLRLSAMGAHARHAGRFDIAK
                     YYRLKA"
     gene            3394..3834
                     /locus_tag="VFL84_05655"
     CDS             3394..3834
                     /locus_tag="VFL84_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608141.1"
                     /translation="MNTTTRRRFFGGAVVLATPLAASAAVTRDDIAARLAALEDASSI
                     RALLRRHVLSINRRAGAAPETHVRSVALDDEATVDVASDGTAAAQVLCTVQTAKPIAG
                     DGTLVEMARLQGDGVVKRSERRVLFGRFVKRNGVWALESAELRT"
     gene            3831..>4398
                     /locus_tag="VFL84_05660"
     CDS             3831..>4398
                     /locus_tag="VFL84_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7608142.1"
                     /translation="MKKIVAAIGLVMVASVGATLVAQTAGAPPKLTAQDLLDIRQLID
                     AYPHLLDNCTNNGNDYADLYTPDATFGVSQEWGKAKIWFRGREQLRRAGGGTDTGCRP
                     SQSQGHAYHLNINPTITATPTGAHAISTLLTITNDTGSRGDIVHWEGGYEDTFEKTPA
                     GWKFKSRVHVWPEVQWTDRVEDMPARKLE"
BASE COUNT          844 a         1476 c         1452 g          626 t
ORIGIN      
        1 gccgaagaca atgcgcgcgc tcagcttcgc gcggcgcgtc aagcgtacgc cggcgtcatt
       61 cgtgaaaaga tccggacatc cgcgacatac caacggcgcg tctccgctgg ggtcttcgcc
      121 aacaggtgat gtcgaccttt ctcgcatacc gccgggaggc gccccatgaa atcggaagaa
      181 gccaagccat tgcaggtggc cgcacaacat gccgcgaact ggtgcatgga aaaactagag
      241 atgaaagaag gcggtttcgg caaagatcac gcgcattcgg attccgagag agcggcgttg
      301 cacgtcgcac actcgatcgc catttgcgag attgcgcgcc ttcagtcgat cgtcgacggc
      361 cgctgaccgc cgcgtagccc tattcagtag accgcgcgac cagcgcgagc cacgcgatcc
      421 cggccagcgc gatgagcagc gccggcgcca tcgcgtactg cacggccgtc caaccgtagg
      481 tgctgagcag cgcgcccgag ccgaacgagc cgaccgcgac gagaccgagc acgaggaagt
      541 cgttgagccc ctgcactttc gcttgctcgg cgcgcgtgtg cgtcgtgccg agcaagctcg
      601 tcgcgccgac gtacgagaag ttccagccga tgccgagcgc gatcagcgcg agatagaaat
      661 gatgcagctc gacgccggcg accgcgatcg cgccgcacac gccgagcagc agtaggccgg
      721 cgacgatgac gggcaggtaa ccgaagcgca cgatcagcga gcccgtgaaa aagctcggcg
      781 cgaacatcgc gacgatgtgc caccgcacca cgtcggtcgc gttgccgtgc gtcaagccat
      841 ggtccaccat cgcgagcggc gtcgacgtca tgacgaggct catcgccgag aagccgacca
      901 tcgccgcgag cacggcgacg acgaacgcgg gttgccgcgc gatctcggcg agcggccggc
      961 cggtctcggc cgccttcgcc gcgcgcggcg gaatcgggat gtcgagaaac ttgagaccga
     1021 tcgcgccgac gacgttcagc gcgatcatcg cgatgtacgc gcccgcatac ggcacgttcg
     1081 cgatcgcgtc cgctccgaag tccgcgatct gcgggccgag caacgcgctc aacaggccgc
     1141 cggccagaac ccatgagatc gccttcggct tgaaggcgtc ggatgccgtg tcggtggccg
     1201 cgaagcggaa gaagccttgc gtcgcctgga agacgccgag cagcacggag cccgtcagga
     1261 acagcgcgaa gctgccgacg aacagcgccc gcgcgcacaa ggccgaggca atcgcggccg
     1321 cgagcgtcgc gacccagaac cccgtgcgcc ggccgtagcg gctcatgaac aacgacatcg
     1381 tcggcgccgt cagcagcgag ccgagcacga cgatcgagat cggcaacgtg gcaaggctcg
     1441 ggtccggggc cagcaccttt cccgcccagc cggcgacgat gatgttcgtc gcgagctgcg
     1501 cccccaagat cgcttgcgcg aacacgagca cggcgacgtt gcgcttggcg cgcccgtcgt
     1561 ccggcacggc ggcgatatcg gcgatcgggg tactcacggc aataggtcac caacgcggcg
     1621 cgccaggtta ccatacgcca gcgagccaaa cggggggacg ccgccgtgca aacaatcgcc
     1681 tatccaaaag agcagctgca ccgcatcagc ttcacgaagc tgaccgaagc gcagattgcg
     1741 tcgaagctcg ggccgacgcc ggcgccgaag agcgcctcgc cgctctccac cgcattggcc
     1801 ggcaagtcgc tgcggatcgt caccgacaac ggcccttcac tccgctatcg gttcacgggc
     1861 aacaaccgtt tgtcgctcgt cgagaacgac ggccgggccg tcgaggccga ctacggcgcg
     1921 ctcacgctcg atcacgtgac gttgttctcg cacttgctcc agggcacgca gcgcggctac
     1981 cacgtcgtcg tcgatcacgc gagcggtctc gcaaccgtgt tcgaagtctg gttctccgac
     2041 ttcacggaca gccgcgaggt gcagcgcgag atctacttcg gctacgtcga ggaggccggc
     2101 aaggaagcgc cgaaggcgcg gcactcgctg acgaatcgcg tcgaaggcaa gggtttccat
     2161 tggacgcagg acaccggcgt cgagacgctc gagctttatc cttccacgtt ctactcgaac
     2221 ttcgtcgagc tctcgcggca aggcggcgag ctcggcttct gcgcgccgac cgactacgtc
     2281 aagatcgacg acgagctcta cgtctattcg cgtgtcgagt gcgagttctc cggcgtgcag
     2341 acgctgtacg tcttcgacgc caatcgcgcc gagcaagtcg gcgtgcggct cggcttcgac
     2401 gctaacgaca cgctcgagta ctacctgttc acgggccgag gcgaatgggt aggccagctc
     2461 gcgcaattcg agaagttcgg cgacacggcc gccacgccga tgccgcctcc cgcgaacggc
     2521 gccccgctgg ccaagggcgc gcgccgcgtc taccggccca tgcgcaacaa ctggcagatg
     2581 accaaagagg aagtcgcggc gctgaccgag aagaacgtgt tcggcgcgcc gagcgcgatg
     2641 gccgggaaca agagccccgt cagcgactac ctcgtcggca aagagctcac gctgcgtttc
     2701 gacaacggcg tcgccgccga ctaccgcttc gacaagatcg acacgctgca ctggcgccgg
     2761 ccgggcgaga gcacgtggca cgaggagcgc tatgaagcct gggagagcgc gcccggcgtc
     2821 gtgatgttcg gccacctgct gaccggcgcg ccgcagaagg actgctacaa gctcgtcgcc
     2881 gatttcgacc atgggctcgt cacgtgcatc cacggcacga tcggcagccg ctacatcgcg
     2941 aacgaggcgc aggcggaaac gtggttcggc gtcgtcgaga tggaaggcat cgtgccgccg
     3001 aagtatcgcc gccaccactt cacggacgag ctcgtcggcc gcgcgatcac gtggaactac
     3061 tcgccgggct tgacctcgat gcacctctac acgacgccgc attcactctc ctggatcatc
     3121 ttcggcgaga gcggtgccgg cggcatggaa tggagcgggc ccgcgtcgta cgtaaagatc
     3181 cgcgacgagc tgtacctcgc gtactggctc gaggaagcct gcaacggcac gctcggcacg
     3241 atcctcgtga acctgcgcac gatgcacgat tgcggcatcg gctatcactg cggccgcgac
     3301 ggcttgaggc tctccgcgat gggcgcgcac gcgcgccacg cggggcggtt cgacattgcg
     3361 aagtactatc ggttgaaggc ctgaggtagc ggcatgaata cgacgactcg acgcagattc
     3421 ttcggcggcg ccgtggtgtt ggcgacgccc ctagcggcgt ccgccgccgt cacgcgtgac
     3481 gatatcgccg cgcggctcgc ggcgctcgag gacgcgagct cgatccgcgc gctgttgcgc
     3541 cgtcacgtct tgagcatcaa caggcgcgcc ggtgcggcgc ccgaaacgca cgtccgcagc
     3601 gtcgcactcg acgacgaggc cacggtcgac gtcgcatccg acggcacggc ggcggcgcag
     3661 gtcctttgca ccgtccaaac tgcgaagccg atcgccggcg acggcacgct cgtcgagatg
     3721 gcacgattgc aaggcgacgg cgtcgtgaaa cgcagcgagc ggcgcgtgct gttcggccgc
     3781 ttcgtcaaga ggaacggagt ttgggcgctc gagagcgcgg agctacgcac atgaaaaaaa
     3841 tcgtcgcggc aatcggactc gtcatggtcg catcggtcgg cgccacgctg gtcgcgcaaa
     3901 cagcgggcgc gcccccgaag ctcacggcgc aagacctgct cgacattcgt cagctcatcg
     3961 acgcgtaccc gcatctgctc gacaactgca cgaacaacgg caacgactac gccgacttgt
     4021 acacgccgga cgcgacgttc ggcgtatcgc aggagtgggg caaagcgaag atctggttcc
     4081 gcggccgcga gcagctgcgc cgcgccggcg gcggcaccga cacgggttgc cggccgagcc
     4141 agtcgcaagg ccacgcgtac cacttgaaca tcaacccgac gatcacggcg acgccgaccg
     4201 gcgcgcacgc gatctcgacg ttgctcacga tcacgaacga cacggggagc cgcggcgaca
     4261 tcgtgcactg ggaaggcggc tacgaggaca cgttcgagaa gacgccggcg ggttggaagt
     4321 tcaagtcgcg tgtgcacgtc tggcccgagg tgcagtggac ggatcgcgtc gaggacatgc
     4381 cggcgcgcaa gctcgagg
//
LOCUS       DASSNN010000311         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_187655, whole genome shotgun sequence.
ACCESSION   DASSNN010000311 DASSNN010000000
VERSION     DASSNN010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187655"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VFL84_05665"
     CDS             <1..1250
                     /locus_tag="VFL84_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HET7608143.1"
                     /translation="RKRYYVDYTGCGNTLDVRHSRALQLVMDSLRYWVQEMHVDGFRF
                     DLGTILAREPNGFDYRSGFLKVCSQDPVLTGVKLIAEPWDCGPGGYQMGEFPPGWGEW
                     NDKFRDTVRDYWRGEAPAAALAPRLYGSADIFDRNGRRPQASVNFVTAHDGFTLNDLV
                     SYNERHNEANGEENRDGHVDNRSWNCGVEGPTDDEAINALRKQQMRNFLATLLLSQGT
                     PMLTAGDEFARTQGGNNNAYCQDNEISWVDWTLAETNIAQVRFVQALTALRRRYPILR
                     RNRFLSTVVNEAIGVKELTWINSAGVEMQEEDWTNGQRCFGMLLDGRAQVTGLKQRGQ
                     DATLLLILNSHHEPVPFTLPGAGDDSAHALWTLVLDTQIAEPAQTAEDEHSAGDTRSV
                     AARSLLLLKLKLPPEAPLWSEQR"
     gene            1291..>2223
                     /locus_tag="VFL84_05670"
     CDS             1291..>2223
                     /locus_tag="VFL84_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET7608144.1"
                     /translation="MSAPLATYRLQLNAELGFAAAAALAPYLQRLGVTHVYTSPILKA
                     RPGSMHGYDIVAHDALNPELGQAAQFDAMVAAFTGARLKVLLDFVPNHMGVGGADNPL
                     WLDVLEWGPESAYAGWFDIDWEPHRGYLHDKLLVPVLGDQYGIELDAGRLELKLDEAA
                     GELAVWAYDHHKLPICPLTYGDVLGDRHPALERLGDEFGALMQWKPQVARRAAELKRA
                     LAAAIRGEEGVRAALIDAVERVNRPDEDGVRRALDAVIRKQYWRAAHFRVAGDDINYR
                     RFFNINDLAGLRIELPEVFEHVHALVLVLLGNRVV"
BASE COUNT          380 a          742 c          766 g          335 t
ORIGIN      
        1 agcggaagcg ctactacgtg gattacaccg ggtgcgggaa caccctcgac gtgcggcatt
       61 cccgtgcgct tcagctggtg atggacagcc tgcgctactg ggtgcaggag atgcacgtcg
      121 acggattcag attcgatctc ggcacgatcc tcgcccgcga gccgaacggc ttcgactacc
      181 gcagcggatt cttgaaagtc tgctcgcagg accccgtcct aaccggtgtg aagctgatcg
      241 cggaaccgtg ggactgcggc ccgggcggct atcagatggg cgagttcccg ccgggttggg
      301 gcgagtggaa cgacaaattt cgcgacacgg tgcgcgacta ctggcgcggc gaagcgccgg
      361 ccgcggcctt ggccccgcgg ctttacggct ccgccgacat cttcgatcga aacggccggc
      421 ggccgcaggc aagcgtcaat ttcgtcacgg cgcacgacgg gttcacgttg aacgacctcg
      481 tctcctacaa cgagcggcac aacgaagcga acggcgagga gaatcgcgac ggtcacgtcg
      541 acaaccgctc gtggaactgc ggcgtcgaag gcccgaccga cgacgaggcc atcaacgcgc
      601 tgcgcaagca gcagatgcgc aattttctgg cgacgctgtt gctctcgcag ggaacgccga
      661 tgctgacggc cggcgacgag ttcgcgcgca cgcaaggcgg caacaacaac gcctactgcc
      721 aggacaacga gatctcgtgg gtcgactgga cgctcgccga gacgaacatc gcgcaggtgc
      781 ggttcgtgca ggcgctgacg gcgctgcggc ggcgttatcc gatcctgcgc cgcaaccggt
      841 tcctgtcgac ggtcgtcaac gaagcgatcg gcgtcaagga gctcacgtgg atcaacagcg
      901 caggcgtcga gatgcaggag gaagactgga cgaacggcca gcgctgcttc ggtatgctgc
      961 tcgacggccg cgcgcaggtc acgggcctca agcagcgcgg ccaggacgcg acgctgctgc
     1021 tgatcctcaa ttcccatcac gagccggtgc cgttcacgct gcccggagcc ggcgacgact
     1081 ccgcgcacgc gctctggacc ctcgtgctcg atacgcagat cgcggagccg gcgcaaaccg
     1141 ccgaggacga gcactcggcc ggcgacacgc gcagcgttgc ggcgcgctcg ctgctgctct
     1201 tgaagctcaa gctgccgccc gaagcgccgc tctggagcga gcagcgatga gcgcgcgaat
     1261 aaggcgcgcg cccggaggcg cgcgctagtc atgtcggcgc cgctcgcgac gtataggctg
     1321 cagctcaacg cggagctcgg ttttgcggcg gccgccgcgc tggcgccgta cttgcagcgg
     1381 ctcggcgtca cgcacgtcta cacgtcgccg atcttgaagg cgcggccggg cagcatgcac
     1441 ggctacgaca tcgtcgcgca cgacgcgctg aaccccgagc tcgggcaggc ggcgcagttc
     1501 gacgcgatgg tcgccgcgtt cacgggcgca aggctcaagg tcctgctcga cttcgtgccg
     1561 aatcacatgg gcgtcggcgg cgcggacaat ccgctctggc tcgacgtgct cgagtggggc
     1621 ccggagtcgg cctatgcggg ctggttcgac atcgattggg agccgcatcg cggctacttg
     1681 cacgacaagc tgctcgtgcc cgtgctcggc gaccagtacg gaatcgagct cgatgccggc
     1741 aggctcgagc tcaagctcga cgaggccgcc ggcgagctcg ccgtctgggc ttacgatcac
     1801 cacaagctgc cgatctgccc gctgacgtac ggcgacgtgt tgggggatcg gcatccggcg
     1861 ctcgagcggc tcggcgacga gttcggcgcg ctcatgcagt ggaagccgca agtcgcgcgc
     1921 cgagccgcgg agctcaaacg cgcgctcgcc gcggcgatcc gcggcgagga aggcgtgcgc
     1981 gcggcgctga tcgacgcggt cgagcgcgtc aaccggcccg atgaggacgg tgtgcgccgc
     2041 gcgctcgatg ccgtcatcag gaagcagtac tggcgcgccg cgcacttccg ggtcgccggc
     2101 gacgacatca actaccgccg gttcttcaac atcaacgacc tcgcgggtct ccgcatcgag
     2161 ctcccggagg tcttcgagca cgtgcacgcg ctcgtgctcg tgctgctcgg caaccgcgtc
     2221 gtc
//
LOCUS       DASSNN010000312         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_187723, whole genome shotgun sequence.
ACCESSION   DASSNN010000312 DASSNN010000000
VERSION     DASSNN010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187723"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VFL84_05675"
     CDS             <1..881
                     /locus_tag="VFL84_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HET7608145.1"
                     /translation="GANGRAARAFYWVPMRLLPSDLESVLDNEFELDRGIRDSSFAGV
                     FYQFPAFTNQHRLEAYVFDADVASASDPALAFDLVTLGARAYRAPQASQWNYEVEAAL
                     QDGESGGTVGGVARSDLDHGASFLHFEIGYQFDAPGSPNLMFQYDRATGDEDPTDAKI
                     DRFNTLFGARRFDFGPTGIYGIAARSNIESPGVRVTFRPAPKWQGMVAYRSLRLEAPR
                     DAWAGSGWRDTSGAAGDSIGTHVEGSFTWAAIPERLSFETGFAHLWAGRFAEQTAGAA
                     FRGDPQYYYFALTTTF"
     gene            899..>2825
                     /locus_tag="VFL84_05680"
     CDS             899..>2825
                     /locus_tag="VFL84_05680"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HET7608146.1"
                     /translation="MRSRSTIALFPVFLLAAGAASADEGMWTFDNFPRELVRERFGVT
                     IDDGWLDNVRLATVRLSGCTGSFVSPNGLILTNHHCVVSCLAQRSTRERSLIDLGFVA
                     NGREGEIDCPTQIADVLERLENVTEKVQAATRGLDERDANERRRQTLTELEQACETTS
                     RNSGNPLKCEAVTLYNGGQYWLYQYHRYDDVRLVFAPEDDIAAFGGDPDNFQFPRWDL
                     DFSLLRAYENGAPAATPRHLTINFAGPAAGDPVFVPGHPGSTDRQLTVAELEALRSRV
                     LPPALLRASELRGRYIQFAKNGDEQRRLVQDVLFSLENSIKVQRKQLDALLEDALLAA
                     KRDEQANLRARVNRNRALAAEVGDPWKKIEDALAVEAARFLPYQFIEGGTGFNSRLWR
                     YARSLVRAAAERPKPNGERLREYTDSFLPRLEQQLAAPVPIYPEVEQLTLSFSLERMR
                     EWLGPDAPVVRRLLATDSPDDVAARLVTGSTLADPAVRMQLWNGGQAAIDASTDPFIV
                     LARSLDAEARELRKWHEDEVEAVVEAAHEQIARARFQIFGTSVYPDATFTLRLNFGTV
                     QGWIENGTPVEPFTYLDRLFERATGQPPFRIPLSWLAARAALDPRTPFNLSTNNDIVG
                     GNSGSPLVAADGRIVGLL"
BASE COUNT          441 a          984 c          953 g          447 t
ORIGIN      
        1 cgggcgccaa cggccgcgcc gcgcgcgcgt tctattgggt gccgatgcgg ctgctgccgt
       61 ccgatctcga gagcgtgctc gacaacgaat tcgagctcga tcgcggcatc cgggacagct
      121 cgttcgcggg agtgttctac cagttcccgg cgttcacgaa tcaacaccgg ctcgaggctt
      181 atgtgttcga cgccgacgtc gcgtcggcga gcgacccggc ccttgcgttc gacctcgtga
      241 cgctcggcgc gcgcgcgtat cgagcgccgc aagcctcgca atggaactac gaggtcgagg
      301 ccgcgctcca ggacggcgag tccggcggca ccgtgggcgg cgtcgcgcgc agcgatctcg
      361 accacggcgc gtcgttcctg cacttcgaga tcggctatca gttcgacgca ccgggctcgc
      421 cgaacctcat gttccagtac gaccgcgcga ccggcgacga ggatcctact gacgcgaaga
      481 tcgatcggtt caatacgttg ttcggcgccc gccgcttcga cttcggcccg accggcatct
      541 acggcatcgc ggcgcgcagc aacatcgagt cgccgggcgt gcgcgtgacg ttccgccccg
      601 cgccgaagtg gcaaggcatg gtcgcttacc gctcgctgcg gctcgaagca ccgcgcgacg
      661 cgtgggcggg gtccggttgg cgagacacgt ccggagccgc cggcgactcg atcggcacgc
      721 acgtcgaagg cagcttcacg tgggcggcca ttccggagcg attgagcttc gagaccggct
      781 tcgcgcattt gtgggccggg cgctttgcag agcagaccgc gggcgcggcc ttccgcggcg
      841 atccgcagta ttactacttc gcgctgacga cgacgttctg agcacaggag gtgcagccat
      901 gcgtagccgc tcgacgatcg cgttgtttcc ggtgttcctg ctcgccgcgg gcgcggcttc
      961 ggccgacgaa ggcatgtgga cgttcgacaa tttcccgcgc gagctcgtcc gcgagcgttt
     1021 cggcgtcacg atcgacgacg gttggctcga caacgtccgg ctcgccacgg tccggctttc
     1081 gggctgcacg ggctcgttcg tctcgccgaa cggcttgatc ctgacgaacc atcactgcgt
     1141 cgtgagctgt ctcgcgcagc gctcgacgcg cgagcgcagc ctcatcgatt tgggcttcgt
     1201 cgcgaacggc cgcgagggcg agatcgactg cccgacgcag atcgccgacg tgctcgagcg
     1261 gctcgagaac gtgaccgaga aagtccaggc ggcgacgcgc gggctcgacg agcgtgacgc
     1321 gaacgagcgc cgccgccaga cgctgacgga gctcgagcag gcctgcgaga caacatcacg
     1381 aaacagcggt aacccattga aatgcgaagc cgtgacgctg tacaacggcg gccaatattg
     1441 gctctaccaa taccatcggt acgacgacgt ccggctcgtc ttcgcgcccg aggacgacat
     1501 cgcggccttc ggcggcgacc ccgacaactt ccagttcccg cgctgggatc tcgacttctc
     1561 gctgctgcgc gcctacgaga acggcgcgcc ggccgcgacg ccgcggcatc tcacgatcaa
     1621 tttcgccggg ccggccgccg gtgaccccgt cttcgtgccg gggcaccccg gctcgacgga
     1681 ccggcagctc acggtcgccg agctcgaggc gctgcgcagt cgcgtgctgc ctccggcgct
     1741 gctgcgcgct tcggagctgc gtgggcgcta catccagttc gcgaagaacg gcgacgagca
     1801 gaggcgcctc gtgcaagacg tgctgttcag cctcgagaac tcgatcaagg tccagcgcaa
     1861 gcagctcgac gcgctgctcg aggacgcgct gctcgccgcc aaacgcgacg aacaagccaa
     1921 cctccgcgcg cgcgtgaatc gcaaccgcgc cctcgccgcc gaggtcggcg acccgtggaa
     1981 gaaaatcgag gacgcgctcg ccgtcgaggc ggcgcgtttc ctgccgtacc agttcatcga
     2041 aggcggcacc ggcttcaaca gccgattgtg gcgctatgcg cgatcgctcg tgcgcgcggc
     2101 cgcggaacgg ccgaagccga atggcgagcg cctgcgcgag tacacggaca gcttcctgcc
     2161 gcggctcgag cagcagctcg cggcaccggt cccgatttat cccgaggtcg agcagctgac
     2221 gctgtcgttc tcgctcgagc gcatgcgcga gtggctaggc cccgacgcac cggtcgtgcg
     2281 ccgcttgctc gcgaccgaca gccccgacga cgtcgcggcg cggctcgtga cgggctcgac
     2341 gctcgccgat cccgccgtgc gcatgcagct ctggaacggc ggccaggccg cgatcgacgc
     2401 gtcgacggat ccgttcatcg tgctcgcgcg ttccctcgac gccgaggcgc gcgagctgcg
     2461 caagtggcac gaggacgagg tcgaggcggt cgtcgaagcc gcgcacgagc agattgcgcg
     2521 ggcgcgcttt cagatcttcg gcacgagcgt ctatcccgac gcgacgttca cgctgcgact
     2581 gaacttcggc accgtgcagg ggtggatcga gaacggcacg cctgtcgagc cgttcacgta
     2641 tctcgaccgg ctgttcgagc gcgcgacggg gcagccgccg tttcgcattc cgttgagctg
     2701 gctcgccgcg cgcgccgcgc tcgatccgcg cacgccgttc aacctctcga cgaacaacga
     2761 catcgtcggc ggcaactcgg gcagcccgct cgtcgcggcc gacgggcgca tcgtcgggtt
     2821 gctgt
//
LOCUS       DASSNN010000313         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1680087, whole genome shotgun sequence.
ACCESSION   DASSNN010000313 DASSNN010000000
VERSION     DASSNN010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680087"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..832
                     /locus_tag="VFL84_05685"
     CDS             383..832
                     /locus_tag="VFL84_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608147.1"
                     /translation="MLRRRGFFTDLVYWFVTPLLTRTVSPIGLALILIVVYRDDIAGS
                     RAMLLERDTLLARSRLAFRRAAAALGWLVASPKFHGWHHTSEDEGLDKNFAGLLPLFD
                     VVFGTYYMPKGRLPERFGLARESIPATIRGQMAYPFRRTKLTQRASP"
     gene            893..1681
                     /locus_tag="VFL84_05690"
     CDS             893..1681
                     /locus_tag="VFL84_05690"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylmalonate decarboxylase"
                     /protein_id="HET7608148.1"
                     /translation="MSMHRRELIQSGIAAGFLAALKLPALAAEAPTVGLIFPPANYPI
                     PPDAGRLYPSGVRFIGNGLGFNGMTIESYNEAIPRALPRAQELAAQGANLISVFGSSI
                     TFYKGAAFNKELTDKVTELTGLPATTQSNGLVDGLKHVNAKRVAVATAYTDIVTERLK
                     VFLTEHGFEVLSTKGLGYERIPEGAATPEILHKLGVDAHAAAGKPDALVMSCGGLRTL
                     DVIVPLEKATGVPVVSSTPHGLWHCARMVGVKNKIEGFGMVMAA"
     gene            complement(1702..>2147)
                     /locus_tag="VFL84_05695"
     CDS             complement(1702..>2147)
                     /locus_tag="VFL84_05695"
                     /inference="COORDINATES: protein motif:HMM:NF039957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6010 family protein"
                     /protein_id="HET7608149.1"
                     /translation="MVWYVGLGALAATGAMYLSHRFVPAKHEATFYGLIFIAIAAVYL
                     AFNSYLGDAAAWRLETVAVVAFAVMGCVGTRVPAVLIAGYVLHGGWDLLHEIQAHMQV
                     DVFAGRAATHIPLAYGAFCATFDWVIPVYFVHRRRHWAAAWKSRP"
BASE COUNT          379 a          719 c          678 g          371 t
ORIGIN      
        1 ctgcggttgt tcgtggacgt gtccggttgc agctccgtgc aagccggcgt ggtgctcgat
       61 aggcgccgcg gacgtggcca tcgcgaagca cggcgccgct tgaaggctga ccgaagcgaa
      121 cgcgaacaac cccacgacga agcgaccaag actcgctcgg cgccgtcgaa ggattcggaa
      181 gatgttgccg gatctcctca ttggcttagc gtagccgtta tgtagtgcat catccaagcg
      241 cggaagcgcg acgcgcgtca actgccggga ggtgcgccgc gtttggagtg gcatatggaa
      301 cgactcggca cgttgttcgt cgcctgctcg ccctctcggc ctgcttctgg cgttgtttgc
      361 ggccaaccct cggcaaccgc gcttgctgcg caggcgcggg ttctttaccg acctcgtcta
      421 ctggttcgtg acgccgctcc tgacgcgcac cgtgtcgccg atcggcctcg cgctgatcct
      481 gatcgtggtc tatcgcgacg acatcgcggg cagtcgcgcg atgctgctcg agcgcgacac
      541 gctactcgcg cgatctcgat tggctttccg ccgtgcggct gcagctctcg gctggctcgt
      601 cgcttcgccg aagtttcacg gctggcacca cacgtccgaa gacgaaggcc tcgacaagaa
      661 tttcgcgggg ctcttgccgc tcttcgacgt cgtgttcggc acttactaca tgccgaaagg
      721 ccgcctgccc gaacggttcg ggctcgcgcg cgagtcgatc cccgcgacga tccggggcca
      781 gatggcctac ccgtttcgcc gcacgaagct tacgcaacgt gcgtcgcctt gacgccgttc
      841 aggcgtttgc gtagcgtcga tccgagtcgc ggacgcaaaa ggggttgcgc cgatgtcgat
      901 gcaccgtcgt gagctcattc aatccggaat cgcggcgggc tttctcgcgg cgctgaagct
      961 gccggcgctg gcggccgagg caccgaccgt gggcctgatc ttcccgcccg ccaattaccc
     1021 gattccgccc gacgccggcc gcctctatcc gagcggcgtg cgcttcatcg gcaacggcct
     1081 cggcttcaat ggcatgacga tcgagagcta caacgaggcg atcccgcgcg cgctgccgcg
     1141 cgcgcaagag ctcgcggcgc agggcgcgaa cttgatctcc gtgttcggct cgtcgatcac
     1201 cttctataaa ggcgccgcgt tcaacaagga gctcacggac aaagtcaccg agctcacggg
     1261 gctacccgcg acgacgcaga gcaacgggct cgtcgacggc ttgaagcacg tcaacgcgaa
     1321 acgcgtcgcc gtcgcgacgg cgtacacgga catcgtcacc gagcggctca aggtgttttt
     1381 gaccgagcac ggcttcgagg tcctgtcgac gaaaggcctg ggctacgagc gcatccccga
     1441 gggcgccgcg acgccggaga tcctgcacaa gctcggcgtc gacgcgcacg cggcggccgg
     1501 caagcccgac gcgctcgtga tgtcgtgcgg cggcctgcgc acgctcgacg tcatcgtgcc
     1561 gctcgagaag gccaccggcg ttcccgtcgt ctcctcgacg ccgcacggcc tctggcattg
     1621 cgcgcgcatg gtcggcgtca agaacaagat cgaaggcttc ggcatggtca tggcggcgta
     1681 agagccgcca acggcatcgg actacggtcg tgatttccag gcggcagccc aatggcggcg
     1741 ccgatgcacg aaatacaccg ggatgaccca gtcgaacgtc gcacagaaag cgccataggc
     1801 gagcggaatg tgtgtcgccg ctcttcctgc gaagacgtcc acctgcatgt gggcctgaat
     1861 ctcatgcaac aggtcccatc ctccatgcag cacgtaaccg gcgatcagca cagcgggcac
     1921 tcgcgtgccg acgcatccca tgacggcgaa tgccactacc gcgaccgttt cgagtctcca
     1981 tgccgccgcg tctcctaaat aggaattgaa agcgagatac accgcggcga tcgcgatgaa
     2041 gatcaagccg tagaacgtcg cctcgtgctt cgcaggcacg aacctgtgcg agagatacat
     2101 cgcgcccgtg gcagccagcg cgccgaggcc gacgtaccaa accatga
//
LOCUS       DASSNN010000314         4658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1441937, whole genome shotgun sequence.
ACCESSION   DASSNN010000314 DASSNN010000000
VERSION     DASSNN010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441937"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFL84_05700"
     CDS             complement(<1..546)
                     /locus_tag="VFL84_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608150.1"
                     /translation="MQGLIRITTGTASAALLALACAARADVVDTRELMETIPVAANEP
                     LVVTVKNILGPIHVAGHDASRVEMHATETVRGDLQADIERARAQMQLRTETEPGRVAI
                     RVRHVGDDGDGRRNSGWDNYSIEYDIDVRVPRGATLQISTVNDGDVTVEDVNGEFELK
                     NVNGAVRLVRAGGNGTINTVNG"
     gene            complement(557..1363)
                     /locus_tag="VFL84_05705"
     CDS             complement(557..1363)
                     /locus_tag="VFL84_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET7608151.1"
                     /translation="MTCEETTPLLMDRLQGDISPENEERLAAHVATCADCRAEVEAMT
                     ETWEDLGSLDDEDVPHERLRARFHAGLAAYEARESRHWTERLLGSFWPRQPALQMALA
                     AALALVGVLVGQQLPSRGDAEVAALRNEVRAVGIALLDHQSASERLLGVKWAQGTARA
                     PEVIGALLERVQYDTNLSVRLAAVDALRADLDNPDVVAGLAMALERQESPLLQVALTD
                     ALLAADEPAVEAVRAVLGRTELDPAVRQYLEMALNEVGAGGDMPRANNEA"
     gene            complement(1360..1914)
                     /locus_tag="VFL84_05710"
     CDS             complement(1360..1914)
                     /locus_tag="VFL84_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7608152.1"
                     /translation="MKETSDRDLMRQVRGGRTVALATLFERHHTRVYGYCLRMTGNRA
                     TAEDLVQDVFMKMLKYKATFKEDSELVPWMFGIARNSCLAHLKRRAVDHLPATQATEE
                     AAAEEAHEELRDERQSELVRQALLKLPAERREVLVLSRYEYKSYDEIARVLDCSVGAV
                     KVRAHRAMKQLREIYLDMAGEVSA"
     gene            2285..4543
                     /locus_tag="VFL84_05715"
     CDS             2285..4543
                     /locus_tag="VFL84_05715"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HET7608153.1"
                     /translation="MNAIRVAARAAVLIGCAGAVNAQPEVAAVSEVASSRLASLAIPH
                     STEAIQIDGVLDDAIWRSALALPLTVETYPRENQTPEVETIAYLVENGDQLLIAFDAR
                     DPEPEKIRAYLRDRDSAFNDDFVGVVLDTFNDERRAFEFFVNPLGVQMDLINDDVNRN
                     ESSSWDAIWDSAGQINERGFAAEMAIPFSQLRFPRTDGDQTWGIDVLRFRPRTNRARI
                     SNNAQDRNRSCYLCQFGKFTGFSNAEPGKAIEVVPTLTATRSDTAPTAPGSLERGDFE
                     TEAGLGVRWGITPDLTLDLTFNPDFSQVEADVAQLEENTTFALSYPETRPFFLEGQDY
                     YSTPLTAVYTRTVADPDAGAKFTGRTGKNTIAMFATNDAITNLLFPGPFGSQTTSLAQ
                     ENNGFVGRFARGFGQTSQIGALVTSREGDGYSNEVVGFDGRYFMNDKHTLRFQYLDSK
                     TEYPAEVATRFGQAQDLEGDAWRVEYRYGSRNWFGQVWHQDLDPTFRADSGFIGRVDL
                     VQDRLQMNRTFYAHEGAWWTEWQLGISGNRSETTDGQFINRSVQPFISFEGKLQSYAE
                     VGFGPRKEYWNGVVYDLDSAYMYGQFRPRSGLNINAQLNRGEQIDFTNSRLADQWYYR
                     PQIDWNATRHLLVRLRYTSDHLSTKEGATIYEAKLTDLRLTWQFNVRSFVRLTLQDQD
                     IERNVSQYLNPLTDASTKTLASQLLYSYKLNPQTVLFAGYSDSQLDDNLTGNLEMTGR
                     TLFFKLSYAWTP"
BASE COUNT          779 a         1567 c         1480 g          832 t
ORIGIN      
        1 gccgttgacg gtgttgatcg tgccgttgcc accggcccgg acgaggcgca cggcgccgtt
       61 gacgttcttg agctcgaact cgccgttgac gtcctcgacg gtcacgtcgc cgtcgttgac
      121 cgtcgagatc tgaagcgtgg cgccgcgcgg gacgcgcacg tcgatgtcgt actcgatgct
      181 gtaattgtcc cagcccgagt tgcggcggcc gtcgccgtca tcgccgacgt ggcgcacgcg
      241 gatcgcgacg cgccccggct cggtctccgt gcgtagctgc atctgcgcgc gagcgcgctc
      301 gatgtcggcc tgcaagtcgc cgcgcacggt ttcggtcgcg tgcatctcga ctctactcgc
      361 gtcgtggccc gcgacgtgaa tcggcccgag gatgttcttg acggtgacga cgagcggctc
      421 gttggccgca accggaatcg tctccatgag ctcgcgcgtg tcgacgacgt cggcgcgggc
      481 cgcgcaggcc aaagcgagta gggcggcaga agccgttccc gtcgtgatgc gaatcaaccc
      541 ttgcatgagc tctcctctac gcttcgttgt tcgcccgcgg catgtcgccg ccggccccga
      601 cttcgttcaa agccatctcc aaatactggc gcacggccgg atcgagctcc gtccggccga
      661 gaactgcccg caccgcttcg acggcgggct cgtcggcggc gagcagcgca tccgtcaaag
      721 cgacctgcag cagcggcgat tcctgccgct cgagcgccat ggcgaggccc gccacgacgt
      781 cggggttgtc gagatcggcg cgcagcgcgt cgacggccgc gagccgcacg ctcaagttcg
      841 tgtcgtactg gacgcgctcg agcagggcgc cgatgacttc gggcgcgcgc gccgtgccct
      901 gcgcccattt gacgccgagc agccgctcgg aggcggattg atgatcgaga agcgcgatgc
      961 cgacggcgcg cacttcgttg cgcagtgccg cgacctccgc gtcgccgcgc gacggaagct
     1021 gttggccgac gagcacgccg accagcgcga gcgccgccgc gagcgccatc tgcagcgccg
     1081 gctgtcgcgg ccagaagctg ccgagcaggc gctcggtcca gtgacgcgac tcgcgcgctt
     1141 cgtacgcggc caaccccgca tggaaccgcg cgcgcagccg ctcgtgcggc acgtcctcgt
     1201 cgtccaagct tcccaagtcc tcccaggtct ccgtcatggc ttcgacctcg gcgcggcagt
     1261 cggcacacgt tgcgacatgc gcggcgagcc gctcctcgtt ctcgggcgag atgtcgccct
     1321 gcagccgatc catgagcagc ggtgtcgtct cttcgcacgt catgccgata cctctcccgc
     1381 catgtcgaga tagatctcgc gtagttgctt catcgctcgg tgcgcgcgca ccttgacggc
     1441 gccgacggag caatcgagca cacgcgcgat ctcgtcatag ctcttgtatt catagcggct
     1501 cagcacgagc acctcgcggc gctcggccgg cagcttgagc aacgcttggc gcacgagctc
     1561 gctctggcgc tcgtcgcgca gctcctcgtg cgcttcctcg gccgccgctt cctcggtcgc
     1621 ttgcgtcgcg ggcagatgat cgacggcgcg gcgctttaga tgcgcgaggc acgagttgcg
     1681 cgcgatgccg aacatccacg gcacgagctc gctgtcttcc ttgaacgtcg ctttgtactt
     1741 caacatcttc atgaacacgt cctgcacgag gtcttcggcc gtcgcgcgat tgccggtcat
     1801 gcgcaggcaa taaccgtaga ctcgcgtgtg atggcgctcg aacagcgtcg ccagggccac
     1861 ggtgcggccg ccgcgcactt gccgcatcaa gtctctgtcg ctcgtttcct tcacgaatct
     1921 atggtcgcct tgccgcttac gtgtttaata ttcgcgaggg ccgtcaaagg ttacacgccg
     1981 gcgctatgta accattcgcc ggttgccgta tctaacggca ggccaccagg ggttcgcctg
     2041 gcgcgtgaag gggggatctc ttggtacgtc cttgggctga ttcgctcgca gcggcggagc
     2101 gcgtcttttc gcccaattcc gcgcgaaatc gccacttatt gtcccgggaa cgcgactccc
     2161 ccgcgctcgt ggagcgctcc acggcgttac gtatcagcga gttaggacgc gggaccgccc
     2221 tggcacgctc gctgcaaaag gcctgcgggc cgagagcgct cgttagggag ggacgttcga
     2281 gccgatgaac gctatacgag tggcggcccg cgccgccgtg ctcatcggct gtgccggcgc
     2341 cgtgaacgcg cagcccgaag tcgcggccgt gtccgaggtc gcctcgagcc gccttgcgag
     2401 cctcgcgatt ccgcattcga cggaagcgat tcagatcgac ggcgtcctcg acgacgcgat
     2461 ttggcgttcc gcgcttgcgc tgccgctgac ggtcgagacg taccctcgcg agaatcaaac
     2521 gcccgaggtc gagacgatcg cctatctcgt cgagaacggc gatcagctct tgatcgcgtt
     2581 cgacgcgcgc gatccggagc cggaaaagat ccgcgcgtac ctgcgcgatc gcgactccgc
     2641 gttcaacgac gacttcgtcg gcgtcgtgct cgacacgttc aatgacgagc ggcgcgcgtt
     2701 cgagttcttc gtcaacccgc tcggcgtgca gatggacctg atcaacgacg acgtcaatcg
     2761 caacgagagc tcgtcgtggg acgccatttg ggattcggcc ggccagatca acgagcgagg
     2821 gtttgccgcc gagatggcga tccctttcag tcagctccgc tttccgcgca cggacggcga
     2881 tcaaacctgg ggcatcgacg tgctgcgttt tcgcccgcgc acgaaccgcg cgcggatctc
     2941 gaacaacgcg caggaccgaa atcgcagctg ctatctatgc cagttcggca agttcacggg
     3001 gttctccaac gcggagcccg gcaaggcgat cgaggtcgtg ccgacgctga cggcgacgcg
     3061 ctcggacacg gcgccgacgg cgccgggatc cctggagcga ggtgatttcg agaccgaggc
     3121 cgggctcggc gtgcgctggg gcatcacgcc ggacctgacg ctcgacctca cgttcaatcc
     3181 cgacttctcg caagtcgaag ccgatgtcgc gcagctcgag gagaacacga cgttcgcgct
     3241 gtcctacccc gagacgcggc cgttctttct cgagggccag gactattact cgaccccgct
     3301 gacggccgtg tacacacgca cggtcgcgga ccccgatgcc ggcgcgaagt tcacgggccg
     3361 caccggcaag aacacgatcg cgatgtttgc gacgaacgac gcgatcacga acctgctgtt
     3421 cccgggcccg ttcggctcgc agacgacctc gctcgcgcaa gagaacaacg ggttcgtcgg
     3481 ccgcttcgcg cgcggattcg gccagacctc gcagatcggg gcgctcgtga cgagccgcga
     3541 aggcgacggc tacagcaacg aggtcgtcgg cttcgacggc cgttatttca tgaacgacaa
     3601 gcacacgctg cgattccagt acctcgactc gaagacggag tatccggcgg aggtcgcgac
     3661 ccgcttcggc caagcccaag atctcgaggg cgacgcatgg cgcgtcgaat atcgctacgg
     3721 gtcgcgcaac tggttcggcc aggtttggca ccaggacctc gaccccactt tccgcgccga
     3781 ctctggcttc atcggccgcg tcgatctcgt ccaggatcga ctccaaatga accgcacctt
     3841 ctacgcccac gagggcgcgt ggtggacgga gtggcagctc ggcatctccg gcaaccgttc
     3901 cgagactacc gacggccagt tcatcaatcg gagcgtgcag ccgttcatct cgttcgaagg
     3961 gaaattgcag tcgtacgccg aagttggctt tggcccgcgc aaggagtact ggaacggcgt
     4021 cgtctacgac ctcgacagtg cctacatgta cgggcagttc cgcccgcgca gcggcttgaa
     4081 tatcaacgcg cagttgaacc gcggcgagca gatcgatttc acgaactcgc gcttggccga
     4141 ccagtggtac taccgtccgc agatcgattg gaacgcgacg cgccacttgc tcgtgcgctt
     4201 gcgctacacg agcgaccact tgagcacgaa ggaaggcgcg acgatctacg aggccaagct
     4261 caccgacttg cgcctgactt ggcagttcaa cgtgcgcagc ttcgtgcgct tgacgcttca
     4321 agatcaggac atcgagcgca acgtgagcca gtacctgaac ccgttgacgg atgcgagcac
     4381 gaagacgctc gcgtcgcagt tgctctactc gtacaagctg aacccgcaga cggtgctgtt
     4441 cgcgggttat tcggacagcc agctcgacga caacctcacc ggcaacctcg agatgacggg
     4501 ccgcacgctg ttcttcaagc tgagctacgc ctggacgccg tgagccgcgg cgttagagct
     4561 tgaagaagcg ctccgcgttc gtctgataga acgctttcag gtcttcgtag ccgatcggca
     4621 gcgcgtccgt gacgttcact tcctcgggca cgaactgg
//
LOCUS       DASSNN010000315         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_920776, whole genome shotgun sequence.
ACCESSION   DASSNN010000315 DASSNN010000000
VERSION     DASSNN010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920776"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VFL84_05720"
     CDS             complement(<1..900)
                     /locus_tag="VFL84_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7608154.1"
                     /translation="MSLLAVLLVTQILLGGLDNLWHHELKERLPARREARVEVALHAG
                     RELCYALSFAGLAWWEWRGAWAAAVVALLVVEVCVTLADFVVEDRTRRLPSTERVLHT
                     VLAINFGALLAVLAPVILAWARLPTALVRVDYGLASWLLTAAAAGVLAFSVRNALAAY
                     RHLAAATWARCGLKPRRSAAPKTILVTGATGLIGSKLVYRLVRRGDGVIVHARNAAKA
                     ADLFGPHVDIVTDLAQVPADTRIDAVVNLAGAPIAGAPWTRRRRAYLLESRLGVTRAL
                     LELVERLAVKPTTWINASAIGYYG"
     gene            complement(907..1599)
                     /locus_tag="VFL84_05725"
     CDS             complement(907..1599)
                     /locus_tag="VFL84_05725"
                     /inference="COORDINATES: protein motif:HMM:NF025135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HET7608155.1"
                     /translation="MTTHAFFDPFGAFAAEAPAAPARAPRARRNLCYRELLGETWWRL
                     PAATRARFEGHDALYAGTMTLHATAAGRWVARLCKLVGSPLPPPSDRPLAATVRVEPD
                     RGTGGSRWTRCYDFPRGRVSVASVKALDADGGLVERLGCGIRMRLTLGVRDGALCFDS
                     AGYYVESEGVAFGGRRYGAWRIALPSWFLPGRTCVVHSDLGGGRFRFTMTIRHELLGE
                     LFHHDGVFHSVE"
     gene            complement(1783..2691)
                     /locus_tag="VFL84_05730"
     CDS             complement(1783..2691)
                     /locus_tag="VFL84_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009020048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET7608156.1"
                     /translation="MSSGVETEARPEVSTGPAVELTIEPRVRSLGEFDVRRVLPAAKR
                     RMVGPFVFLDHMGPAVFPPGHGIAVRPHPHIGLATITYLFEGEIVHRDSLGVRQLIQA
                     GAVNLMTAGRGIVHSERASDDLAVTSRLHGIQSWIALPQSLEEMEPTFLHYPASALPE
                     MTVDGCTVRMIIGSAYGLQSPVLTYSPTLYFEASLAPNAQLAVLDEAPERAVYVVSGR
                     VAIDGEAYGEGTLAVLRGGVSAALRAETEARLVVVGGSSVGPRHIWWNFVSSSEARIE
                     RAKRDWAEMRMGTVPGDDEYIPLPTN"
BASE COUNT          500 a         1010 c          986 g          404 t
ORIGIN      
        1 gccgtagtag ccgatcgcgg aagcgttgat ccacgtcgtc ggcttcacgg cgagccgctc
       61 gacgagctcg agcagcgcgc gcgtgacgcc gagccggctc tcgagcagat atgcgcggcg
      121 gcggcgagtc caaggcgcgc cggctatcgg cgcgccggcg agattgacga cggcatcgat
      181 gcgcgtgtcg gcgggcacct gcgcaagatc ggtcacgatg tcgacgtgcg gcccgaacaa
      241 gtccgcggcc ttggcggcgt tgcgcgcgtg cacgatgacg ccgtcgccgc ggcggacgag
      301 gcgatagacg agcttgctgc cgatcagccc cgtcgcgccc gtcacgagaa tggtcttggg
      361 cgccgcgctg cgccgcggct tcaacccgca gcgagcccaa gtcgcagccg ctaaatgccg
      421 atacgcggcg agggcgttgc gcacgctaaa ggcgaggact ccggccgcgg ccgccgtcag
      481 cagccacgaa gcgagcccgt agtccacgcg cacgagcgcg gtcggcagcc gcgcccatgc
      541 cagtatcacc ggcgcgagga ccgcgagcag ggcgccgaag ttgatcgcga gcaccgtgtg
      601 cagaacgcgc tccgtgctcg gcagccggcg cgtgcggtcc tcgacgacga agtccgcgag
      661 cgtcacgcag acctcgacga cgagcagcgc aacgacggcg gccgcccacg ccccgcgcca
      721 ttcccaccac gcaagcccgg cgaacgagag cgcgtagcag agctcgcggc ccgcgtgcag
      781 cgcgacctcg acgcgtgctt cgcgtctggc gggcaggcgc tccttgagct cgtgatgcca
      841 gaggttgtcg agaccgccga gcaagatctg cgtgaccagc aagactgcga ggagcgacat
      901 gacttgctac tccactgaat gaaacacgcc gtcgtggtga aacagctcgc cgagcagctc
      961 gtgccgtatc gtcatcgtga agcggaaacg gccgccgccg agatcgctgt gaacgacgca
     1021 ggtccggccc ggcaggaacc acgacggcaa cgcgatgcgc caagcgccat accgccggcc
     1081 gccgaacgcg acgccttccg actcgacgta gtagcccgcg ctgtcgaagc agagcgctcc
     1141 gtcgcgcacg ccgagcgtga gccgcatgcg aatcccgcag cccaagcgct cgactagccc
     1201 gccgtcggcg tcgagcgcct tcaccgatgc gaccgacacg cgcccgcgcg ggaagtcgta
     1261 gcagcgggtc cagcggctgc cgcccgtgcc gcgatcgggc tcgacgcgca cggtcgcggc
     1321 aagcggccgg tcgctcgggg gcggcagcgg cgagcccacg agcttgcaca agcgtgcgac
     1381 ccagcgtccc gccgcggtcg cgtgcaacgt catcgtgccc gcgtagagcg cgtcgtgccc
     1441 ttcgaagcgc gcgcgggtcg cggccggcag ccgccaccaa gtctcgccga gcagctcgcg
     1501 gtagcagagg ttgcgacgcg cgcgcggtgc ccgcgccggg gcggccggcg cttccgccgc
     1561 gaaggccccg aaaggatcga agaatgcgtg cgtcgtcatg gcggaccttc cgtaatttca
     1621 gttaaaacag aacgcgacac gcaaaatcgt ttctcgatca gggccttacg tacagcctgg
     1681 atcggtgccc tgtctcagcg ctcctatcgt taaatcagta atttctgtta ttacagaaat
     1741 aagatacgcg cgtgaagcgc agccgtcaag cccgtacgga cgtcagttcg tggggagcgg
     1801 aatgtactcg tcgtcgccgg gcacagtgcc catgcgcatc tcggcccagt cgcgcttcgc
     1861 gcgctcgatg cgcgcttcgg agctcgagac gaaattccac cagatgtgcc gcggaccgac
     1921 cgagctgccg ccgacgacca cgaggcgagc ttcggtctcg gctcggagcg cggcgctgac
     1981 gccgccgcgc aagacggcga gagtcccttc gccgtacgct tcgccgtcga tcgcgacgcg
     2041 tcccgagacg acgtagacgg cgcgctcggg agcctcgtcg agcacggcga gctgcgcgtt
     2101 cggcgcgagc gacgcctcga agtacaacgt cggcgaatac gtgagcaccg gcgactgcaa
     2161 cccgtacgcg ctgccaatga tcatgcgcac ggtgcagccg tcgaccgtca tctcgggcag
     2221 cgcgctcgcg ggatagtgca ggaaggtcgg ctccatttcc tcgaggctct gcggcaacgc
     2281 gatccacgac tgaatgccgt gcagcctcga cgtcacggca agatcgtcgc tggcgcgctc
     2341 ggagtgcacg atgccgcggc ccgccgtcat caagttgacg gcgcccgctt gaatgagctg
     2401 cctcacaccg aggctgtcgc gatgcacgat ctcgccttcg aacagatacg tgatcgtcgc
     2461 gagcccgatg tgcggatgcg ggcgcacggc gattccgtgc ccgggcggga acacggcggg
     2521 gcccatgtga tcgagaaaca cgaacgggcc gaccatgcgc cgcttggcgg ccggcaggac
     2581 gcgacgaacg tcgaactcgc cgaggctgcg gacgcgaggc tcgatcgtca gctcgaccgc
     2641 ggggccggtc gagacttccg gccgcgcctc cgtttcaact ccgctgctca tgccgaggca
     2701 taatgccaca tggctcgcat gccgactttg ttcatatcgc acgggtcgcc gatgctcgcc
     2761 gtggccgaca gcgccgcgcg gcgcttcctc gcgcagctgg gaccgaagct gccggcgccg
     2821 acttccgtgg tcgtgatatc cgcgcactac gacgcgccga cgaccgaggt caccgccgcg
     2881 gagtggcccg atacgattca
//
LOCUS       DASSNN010000316         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_562655, whole genome shotgun sequence.
ACCESSION   DASSNN010000316 DASSNN010000000
VERSION     DASSNN010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562655"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1235
                     /locus_tag="VFL84_05735"
     CDS             162..1235
                     /locus_tag="VFL84_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HET7608157.1"
                     /translation="MAGEPRVVKEMPSVALAREEFERRFRARFRDPYFDGVELEAIVD
                     AAWRSYSEHRKAPRSRKAGPEFADPDYELSLDWLAARDAIRIAEMQHRDTSLPARILV
                     INGSSRTNQSCPGETSKSYRLAKLAQSAIESRGASVDFLDMSVLVSEYGRVIYPCKAC
                     VSTAMPLCHWPCSCYPNHGLGQTSDWMHDLYPRWVAAHGVMIVAPVNWYHVPSVLKLM
                     IDRLVCADGGNPDPTRTHGKNAAEAKAIELEGWSYPKHLAGRAFAVAVHGDVAGAGEV
                     KHALCDWLEWMGLIRAGGSGALDRYIGYYEPYATSHDALDRDGAVQAETRNSALALLE
                     RVAQIRSGRYERPDKGLEPPRKK"
     gene            complement(1237..2799)
                     /locus_tag="VFL84_05740"
     CDS             complement(1237..2799)
                     /locus_tag="VFL84_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HET7608158.1"
                     /translation="MSHALAMFLGFIAVTLWITWWAARRSSSSSAFFAAGRTLKAWQN
                     GIAVAGDYMSAASFLGIAGIIAFQGYDGFMYSVGWLVAYLTVLLIVAEPLRNAGKYTM
                     ADVLAYRLSPRPVRAMGAVSTLTVSTFYMIAQMVGAGALVSLLLKGTGITYQTAVVGI
                     GVLMIVYVVFGGMLATTWVQIVKAVLLMAGTILLSILVLAHFGFSFGAFFDAIGHVTY
                     TNAQGNAVTQDFMTPGLRYAPPYGALDLISLGMALIFGTAGLPHILVRFYTVPDAQTA
                     RKSVVWAMLLIGGFYILTTFLGFGAATIVGRDFIAANGGNNMSAPLLAQALGGNLFFA
                     FISAIAFATILAVVAGLTISASTSFAHDFWTNVLHGGRERREGEEVRVARVTAFFVGG
                     ISILIAIVLGPTANVAFLVALAFAVAASANLPVIVLSLFWRRFNTAGAVCGLAVGLLS
                     SLVLIAMSPSILKADAIFPLENPGIVSIPLGFLGAVLGTLLRREPQAEQKFTELSVRA
                     NTGLGAERAVSH"
     gene            complement(2799..2930)
                     /locus_tag="VFL84_05745"
     CDS             complement(2799..2930)
                     /locus_tag="VFL84_05745"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HET7608159.1"
                     /translation="MINVAYLFALSQFLMAWIVAWAYVRAAARFDRMARQVIDEGNR"
BASE COUNT          537 a          992 c          994 g          410 t
ORIGIN      
        1 gcacggaccg cgtcgtcggt cccgccgagg cgggccttgg cgacggtctc gacgaggccg
       61 aggaagcgtt ggccgagcac gatcgcggcg aggaggatcg cggcgagcac tgatcgatcc
      121 tcgcggcatg ctccttgcac cttccggcca cggaggtagc gatggccggc gagccgcgcg
      181 tcgtcaagga gatgccgagc gtcgcgctcg cgcgcgagga gttcgagcga cgattccgcg
      241 cgcggtttcg cgacccctat ttcgacggcg tcgagctcga agccatcgtc gacgcggcgt
      301 ggcgaagcta cagcgagcac cgcaaagcgc cgcggtcgcg caaagccgga ccggaattcg
      361 ccgatcccga ctacgagctg tcgctcgact ggctcgcggc gcgcgacgcg attcgcatcg
      421 ccgagatgca gcatcgtgac acgtcgttgc cggccaggat cctcgtcatc aacggctcgt
      481 cgcgcacgaa tcagtcgtgc cccggcgaga cctcgaagtc gtatcgtctc gcgaagctcg
      541 cgcagtccgc gatcgagagc cgcggcgcga gcgtcgactt cctcgacatg tcggtgctcg
      601 tttcggagta cggccgcgtc atttatccct gcaaagcctg cgtctcgacg gcgatgccgc
      661 tctgccactg gccgtgctcg tgctatccga atcacggtct cggccagacg tcggattgga
      721 tgcacgatct ctatccgcgc tgggtcgccg cgcacggcgt aatgatcgtc gctccggtga
      781 actggtatca cgtgccgtcg gtgctgaagc tcatgatcga ccgcctggtt tgcgccgacg
      841 gcggcaatcc cgacccgacc cgcacgcacg gcaagaatgc cgccgaagcg aaggccatcg
      901 agctcgaagg ctggagctac ccgaagcacc tcgcgggccg cgcgttcgcg gtcgccgtgc
      961 acggcgacgt cgcgggcgcc ggcgaggtca agcacgcgct ctgcgactgg ctcgaatgga
     1021 tgggcctgat ccgcgccggc ggcagcggcg cgctcgaccg ctacatcggc tactacgagc
     1081 cgtacgcgac gagccacgac gcgctcgatc gcgacggcgc cgtgcaagcc gagacgcgca
     1141 actccgcgct cgcgctgctc gagcgcgtgg ctcagatccg cagcgggcgc tacgagcggc
     1201 ccgacaaagg gctcgaaccg ccgcgcaaga aatagctcaa tgcgacacgg cgcgctcggc
     1261 gccgaggccc gtgttggcgc gcacgctgag ctccgtgaac ttctgctcgg cctgcggctc
     1321 gcggcggagc agggtgccga gcacggcgcc gaggaatccc agcgggatgc tgacgatgcc
     1381 gggattctcg agcgggaaga tcgcgtcggc cttgaggatg ctcgggctca tcgcgatcag
     1441 cacgagcgac gagaggaggc ctacggccaa gccgcaaacc gcgccggccg tgttgaagcg
     1501 gcgccagaac aaagacagca cgatcacggg caagttcgcc gacgccgcga ccgcgaacgc
     1561 gagcgccacg aggaacgcga cgttggccgt gggaccgagc acgatcgcga tcaagatcga
     1621 gatgccgccg acgaagaacg ccgtgacgcg cgcgacgcgc acctcttccc cttcgcgccg
     1681 ctcgcggccg ccgtgcagca cgttcgtcca gaagtcgtgc gcgaacgacg tcgacgccga
     1741 gatcgtcagg cccgcgacga cggcgaggat cgtcgcgaac gcaatcgcgg agatgaacgc
     1801 gaagaacagg ttgccgccga gcgcctgcgc gagcagcggc gcgctcatgt tgttgccgcc
     1861 gttcgcggcg atgaagtcgc ggcccacgat cgtcgcggcg ccgaagccca ggaacgtggt
     1921 caggatgtag aagccgccga tgagcagcat cgcccacacg acgctcttgc gcgccgtctg
     1981 cgcgtcggga acggtgtaga agcgcacgag gatgtgcggc agccccgccg tgccgaaaat
     2041 cagcgccatg ccgagcgaga tcaagtcgag cgcgccgtac ggcggcgcgt agcgcagccc
     2101 cggcgtcatg aagtcttgcg tgacggcgtt gccttgcgcg ttcgtgtacg tgacgtggcc
     2161 gatcgcgtcg aagaatgcgc cgaagctgaa gccgaagtga gcgagcacga ggatgctgag
     2221 caggatcgtg ccggccatca gcagcacggc cttgacgatt tgcacccacg tcgtcgccaa
     2281 catgccgccg aacacgacgt agacgatcat cagcacgccg atgccgacga cggccgtctg
     2341 atacgtgatg cccgtccctt tgagcagcag cgacacgagc gcgccggcgc cgaccatctg
     2401 cgcgatcatg tagaacgtgc tgaccgtcag cgtcgagacc gcgcccatcg cgcgcacggg
     2461 ccgcgggctc aaccggtacg cgagcacgtc ggccatcgtg tacttgcccg cgttgcgcag
     2521 cggctcggcc acgatcaaga gcacggtgag atacgcgacg agccagccga ccgagtacat
     2581 gaacccgtca tagccctgga acgcgatgat gcccgcgatg ccgaggaacg acgccgcgct
     2641 catgtagtcg ccggcgaccg cgatgccgtt ctgccaggct ttcagcgtgc ggcccgccgc
     2701 gaagaacgcg ctcgagcttg agctcctgcg cgcggcccac cacgtgatcc acaacgtgac
     2761 ggcgatgaag cccaagaaca ttgcgagcgc gtgcgacatc agcggttgcc ctcgtcgatg
     2821 acctggcgcg ccattcggtc gaaacgcgcg gcggcgcgaa cgtaagccca agcgacgatc
     2881 cacgccatca agaactgcga cagcgcgaag agataggcga cgttgatcac gcc
//
LOCUS       DASSNN010000317         3827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1189746, whole genome shotgun sequence.
ACCESSION   DASSNN010000317 DASSNN010000000
VERSION     DASSNN010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189746"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1180)
                     /locus_tag="VFL84_05750"
     CDS             complement(134..1180)
                     /locus_tag="VFL84_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005368583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7608160.1"
                     /translation="MWTLAWWWMLALLPLPLIVRRFVPAEPLDRDAALKAPVASEFAD
                     LMGIRAPDAARRARLAVLAVVWVLAVLAAARPQFVGEPVALPMTGRDLLLSVDLSGSM
                     EEQDFQLNGAWVDRLTALKSVATDFIERRVGDRVGLILFGREAYLQAPLTFDRQTVAT
                     LLDESVIGLAGKETAIGDSIGLAIRTLEDAGVEQGRRVLLLLTDGANTAGAVEPRKAA
                     ELAAQRKMVIYTIGIGADSLTVRSLFGLREINPSADLDEETLTAIAEATGGRYFRARD
                     TAEFAQIYKILDELEPAESDERGFRPITELFYWPLGAAAGLALAAAVFGFAATRWQWS
                     RAALRFTQRGLSRG"
     gene            complement(1184..1696)
                     /locus_tag="VFL84_05755"
     CDS             complement(1184..1696)
                     /locus_tag="VFL84_05755"
                     /inference="COORDINATES: protein motif:HMM:NF025674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HET7608161.1"
                     /translation="MNPSIDLSQLPLRDIHLPGPVGWWPPAFGWWLVAALVLVGCALY
                     GLHYYRARHKRAALKAMTKVRAALEQGAEPVACLQFLSTILRRFAMTSIARAAGEPDV
                     AGLIGERWLKYLDGRWNRTDFSAGLGKRLLAAPYARPNSIERGTAIELTALCTAWLAA
                     QPATPRPRRA"
     gene            complement(1693..2622)
                     /locus_tag="VFL84_05760"
     CDS             complement(1693..2622)
                     /locus_tag="VFL84_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7608162.1"
                     /translation="MALAARTAPSPVQVTLADLVRFRPAGESLKLTAPRTRVAIEGGH
                     VSPYKGRGVEFDESRPYQPGDDLRTMDWRVTARTGKPHTKVFREERNRPVFVWLDLRR
                     PMLFATRGAFKGVRAAEVAALVAWSAVANGDRLGGLVFSETEHHERRPALGSRAALRL
                     LQLVCTESLWQPPVTAETLETDAERALQRLTRVARPGSLIFLISDFRRLGADYERQLR
                     ELAVHCDLLLVHCYDAVEAELPPPGRYRIQGVGRSFSIETANEATRRRYRERFAARHA
                     ALESLCRYPGIRMVDCPTDAEPRSVLNRKFRSR"
     gene            complement(2625..3599)
                     /locus_tag="VFL84_05765"
     CDS             complement(2625..3599)
                     /locus_tag="VFL84_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7608163.1"
                     /translation="MSLQEKFGKLEAHLKRLVVGQERLLNRMLVALLSDGHLLVEGAP
                     GLAKTRAIKVLSHSVEGDFHRLQFTPDLLPADLTGTEIYRPQDGSFVFRQGPLFHNLV
                     LADEVNRAPAKVQSALLEAMGERQITVGSVTYPLPPLFMVMATQNPIEQEGTYPLPEA
                     QLDRFLLHVLVNYPDAAGERAILELNRAEQKRSASDEELFTAPDLVTQKEVLAARLQV
                     LEQHLSEPLNEYIVQIVLATRAPERYGDDLRGVLQYGASPRATIALDRCSRAHAWLAG
                     RDYVTPEDVQGMAHDVLRHRVLLGFEAEASGRTPNEIIDTLLARIGVP"
BASE COUNT          630 a         1327 c         1324 g          546 t
ORIGIN      
        1 cacgtgcggc ctcaaggtgg gctcgacgac gctgcgccag ccgccgacgt cggctcgccc
       61 gcgcagcagc tgccagatca accatgcgcc gacgggcgcg agcagcagcc agagcgggcg
      121 caggaagtgg aactcagcca cgcgacaacc cccgctgcgt gaagcgtagc gcggcgcgcg
      181 accactgcca gcgcgttgcg gcgaagccga agacagccgc ggcgagcgcc agaccggcgg
      241 ccgcgccgag cggccagtag aagagctcgg tgatcggccg gaagccgcgc tcgtcggact
      301 cggccggctc gagctcgtcc agaatcttgt agatctgcgc gaactcggcc gtgtcgcgcg
      361 cgcggaagta gcggccgccc gtcgcctcgg cgatcgcggt cagcgtctcc tcgtcgagat
      421 cggccgatgg gttgatctcg cgcaggccga acagcgagcg caccgtcagc gagtcggcgc
      481 cgatgccgat cgtgtagatg accatcttgc gctgcgccgc gagctcggct gctttacgcg
      541 gctcgacggc gcccgccgtg ttggcgccgt ccgtcaagag cagcagcacg cggcgcccct
      601 gctcgactcc ggcatcctcg agcgtgcgaa tggcgaggcc gatcgaatcg ccgatcgcgg
      661 tctccttgcc cgcgaggccg atcacggact cgtcgagcag cgtggcgacc gtctggcgat
      721 cgaacgtcag cggcgcttgc aggtatgcct cgcggccgaa caggatcagg ccgacgcgat
      781 cgccgacgcg cctctcgatg aagtcggtcg cgacggactt gagcgccgtg agccggtcga
      841 cccacgcgcc gttcagctgg aagtcctgct cctccatgct gcccgagagg tcgacggaca
      901 agagcaggtc tctgcccgtc atcggcagcg cgacgggctc gccgacgaat tgcggccgcg
      961 cggccgcaag aaccgcgagc acccaaacga cggcgagcac cgcgagtctc gcgcgtcgcg
     1021 cggcgtcggg cgcgcgtatg cccatcaagt cggcgaactc ggaagcgacg ggcgctttca
     1081 gcgccgcgtc gcggtcgagc ggctcggcgg gcacgaagcg ccgcacgatc agcggcaatg
     1141 gcaacagcgc gagcatccac caccacgcga gcgtccacat ggtttatgcc cgcctcggcc
     1201 gcggcgtcgc cggctgtgcg gcgagccacg ccgtgcaaag agccgtgagc tcgatcgccg
     1261 tgccgcgctc gatgctgttc ggccgcgcgt acggcgccgc gagcagcctc tttcccaagc
     1321 cggcgctgaa atcggttcga ttccaacggc cgtcgaggta cttgagccag cgctcgccga
     1381 tcaggcccgc gacgtcgggc tcgccggccg cgcgcgcgat cgacgtcatc gcgaagcggc
     1441 gcaggatcgt cgacaagaac tgcaggcacg cgacgggctc ggctccttgc tcgagcgccg
     1501 cgcgcacttt cgtcatggct ttcagcgccg cgcgcttgtg gcgcgcgcga tagtagtgca
     1561 ggccatagag cgcgcagccg acgagcacga gcgccgcgac gagccaccaa ccgaaggcag
     1621 gcggccacca gccgaccggc cccggcagat gaatgtcgcg aagcggcagc tggctcaagt
     1681 cgatcgacgg attcatcgtg agcgaaactt ccggttcaac acgctgcgcg gctccgcatc
     1741 ggtagggcaa tcgaccatgc gaatgcccgg gtatcggcac aaggactcga gcgcggcatg
     1801 gcgcgcggca aaccgctcgc ggtagcgcct gcgcgtcgcc tcgttcgccg tttcgatgct
     1861 gaacgaccgc ccgacgccct ggattcgata gcggcccggc ggcggaagtt cggcctccac
     1921 tgcatcgtag cagtggacga gcagcaggtc gcagtggact gcgagctctc tgagctgccg
     1981 ttcgtagtcc gcgccgagcc tgcggaagtc gctgatcaga aagatcaagc tgccggggcg
     2041 ggcgacccgc gtcagccgct gcaaggcgcg ctcggcatcg gtctccaggg tctccgcggt
     2101 cacgggcggc tgccagagcg actccgtgca gacgagctga aggagccgta gcgccgcgcg
     2161 cgacccgagc gccggccggc gctcgtgatg ctcggtctcg gagaagacga gccccccgag
     2221 gcggtcgccg ttcgcgacgg cgctccacgc gacgagcgcc gcgacctcgg ccgcgcgcac
     2281 gcctttgaac gcgccgcgcg tcgcgaacag catcgggcgg cgcaagtcga gccagacgaa
     2341 cacgggccga ttgcgctcct cgcggaacac cttcgtgtgc ggcttgccgg tgcgcgccgt
     2401 gacgcgccag tccatcgtgc gcagatcgtc gccgggttga tacgggcgcg actcgtcgaa
     2461 ctcgacgccg cggcccttgt acggcgagac gtgccctcct tcgatggcga cgcgagtgcg
     2521 cggcgccgtc agcttcagcg actcgccggc cggccggaag cgcacgaggt ccgcgagcgt
     2581 gacctgcacg ggcgaaggtg cggtgcgcgc ggcgagcgcc atcgtcaggg caccccgatc
     2641 ctcgcgagca gcgtgtcgat gatctcgttc ggcgtgcgcc ccgaagcttc ggcctcgaag
     2701 ccgagcagga cgcgatgccg cagcacgtcg tgcgccatgc cctgcacgtc ctcgggcgtc
     2761 acgtagtcgc gtccggcgag ccacgcatgc gcgcgcgagc agcgatcgag cgcgatcgtc
     2821 gcgcgcgggc tcgcgccgta ttggagcacg ccgcgcaagt cgtcgccgta gcgctccggc
     2881 gcgcgcgtcg cgagcacgat ctgcacgatg tactcgttga gcggctccga cagatgctgc
     2941 tcgagcacct gcaaccgcgc ggccagcact tccttctgcg tcacgaggtc gggcgcagtg
     3001 aacaattctt cgtcggacgc cgagcgcttc tgctccgcgc gattgagctc gaggatcgcg
     3061 cgctcgccgg ccgcatcggg atagttgacg agcacgtgca gcaggaagcg atcgagctgc
     3121 gcctcgggca gcgggtacgt cccttcctgc tcgatcgggt tctgcgtcgc catgaccatg
     3181 aacagcggcg gcagcggata cgtcacggag ccgaccgtga tctgccgctc gcccatcgcc
     3241 tcgagcagcg ccgattgcac tttcgccggc gctcggttga cctcgtcggc gagcacgagg
     3301 ttgtggaaca gagggccctg ccgaaacacg aacgagccgt cctgcggccg atagatctcg
     3361 gtgccggtca ggtccgcggg caagaggtcg ggcgtgaact gcaggcgatg aaagtcgcct
     3421 tcgacgctgt gcgagagcac tttgatcgcg cgcgtcttgg cgaggcccgg cgcgccctcg
     3481 acgagcaagt gcccgtcgct gagcaaagcg acgagcattc gattcagcaa ccgttcttga
     3541 ccgacgacga gacgcttcag gtgcgcttcg agcttcccga acttttcttg taacgacatg
     3601 aagttaagcc ggacctaaag cgggccattc tagccagcgc aggaaggcct aaccactcgc
     3661 ggtggaaact taaggtcagc gcaacgggaa gacggcgggc gggccccgaa agcccccggt
     3721 aatgccgatc ggcccgcgcg gcacgacgag ctgcacgttc tggccgtccc gccgaatgtc
     3781 gaggacgacg gactcacccg gcgtgccctc gagagtcaac gcgttca
//
LOCUS       DASSNN010000318         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_800178, whole genome shotgun sequence.
ACCESSION   DASSNN010000318 DASSNN010000000
VERSION     DASSNN010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800178"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1253
                     /locus_tag="VFL84_05770"
     CDS             219..1253
                     /locus_tag="VFL84_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7608164.1"
                     /translation="MLGRFRQWTLHVAPVAVAALALLSFTAAAQEPAADGPAAAPHIT
                     LIELTPHLAVNDAAALIEVGKYADAIMLLDEFTANQSQQVPEAFYLLGLAHYQLGDYA
                     KARMPAERAATLAPDAPLSWLELVADILKRSNQPRAAIPWLEKLIDRAPGNKVYWLEL
                     SVAYEKVGDFERSLATMRLAQQAGLLTEDGDFRRLSDLLVHQGLPFQGAEVLDQALAA
                     QTVRADEAAYTKLGTAWFMAGETDKAVLPLENAARAAAGGDGYVRLANVHITRQDWPA
                     AVAALHAGMGKGSLTDEAHADLLMGVALYAQGKFDEAREWLTMAANSERHRAMAQSYL
                     DAIDARIASR"
     gene            1262..1726
                     /locus_tag="VFL84_05775"
     CDS             1262..1726
                     /locus_tag="VFL84_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET7608165.1"
                     /translation="MKKYRFDAEIKEGRGGGAYVVFPYDVEKEFGTSGKVPVDATIDG
                     EPEKSSLMRMGMPQHILGVAKAIREKIGKKPGNVVTIVLWKDDAPREVAVPPELQARM
                     KKAGVLAFFESLSFTHRKEYCRWITEAKKEETRARRLARAIELLTDGVRTPG"
     gene            complement(1723..>2378)
                     /locus_tag="VFL84_05780"
     CDS             complement(1723..>2378)
                     /locus_tag="VFL84_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7608166.1"
                     /translation="ALVRAFANEGYAVVAAARSASTLAELKRANEAARVVAIDSDATG
                     QADVERVFDEAATLGPTEVAVFNAGAFVRGSVLETDPAEFERCWRVGCFAGFLVGQAA
                     ARRMVERGSGTILFTGATASLRGGAGFVSVASPKFGLRAVAQSMARELGPKNIHVAHV
                     IIDGQIRSQRYARLEGERGPDSLLDPDAIAAQYVALHRQHRSAWTQELDLRPWSEKF"
BASE COUNT          439 a          783 c          817 g          339 t
ORIGIN      
        1 gacgaaccgc cacggcgtgc tcgagctgac gatcccgaag caggcaaaag ctttgccgcg
       61 gcgcatcaaa gtcgcggcct gagtgagccg acaagcttag ggagcgatcg gcgattgcgg
      121 accgtccggg gggcaggatg ccccccggaa cgggacccgc ttgtctcgct cgcatgagcg
      181 tagaatctcc atcgacgcgc gataaccggg aaccggtcat gttgggccgc tttcgacaat
      241 ggacactgca cgtcgcaccg gtcgcggttg cggcgctcgc gctgctgtcg ttcaccgcgg
      301 cggcgcaaga gccggcggcg gacggacctg cggcggctcc gcacatcacg ctgatcgagc
      361 tgacgcccca tctcgccgtc aacgacgccg cggcgctgat cgaggtcggc aagtacgccg
      421 acgcgatcat gctgctcgac gagttcaccg ccaaccaatc gcagcaggtg ccggaggctt
      481 tctatctgct cggcctagcg cactatcagc tcggcgacta tgcgaaagca cggatgccgg
      541 ccgagcgcgc ggcaacgcta gcgcccgacg cgcccctgag ctggctcgag ctggtcgcgg
      601 atattctgaa gcgcagcaac cagccgcgcg ccgcgattcc gtggctcgag aaattgatcg
      661 acagagcgcc cggcaacaag gtctattggc tcgagctgtc ggtcgcgtac gagaaggtcg
      721 gcgacttcga gcggtcgctc gcgacgatgc ggctcgcgca gcaggcgggc ttgctcacgg
      781 aagacggcga ctttcgccgg ctctccgatc tgctcgtgca ccaaggtctg ccgtttcagg
      841 gcgccgaggt gctcgaccaa gcgctcgccg cgcaaaccgt gagggccgat gaagcggcct
      901 acactaagct cggcacggcc tggttcatgg ccggcgagac ggacaaggcc gtgctgccgc
      961 tcgagaacgc cgcgcgagcc gcggccggcg gcgacggcta cgtgcggctc gcgaacgtgc
     1021 acatcacgcg gcaagactgg cccgccgccg tcgcggctct gcacgccggc atgggcaagg
     1081 gctcgctgac cgacgaagcg cacgcagatc tcttgatggg cgtcgcgctc tacgcgcaag
     1141 gcaagttcga cgaagcgcgc gagtggctca cgatggccgc gaattccgag cggcaccgcg
     1201 cgatggcgca gagctacctc gacgcgatcg acgcgcgcat cgcctcacgc tgatcaaggc
     1261 aatgaaaaag tacaggttcg acgcagagat caaggaaggc cgcggcggcg gcgcatacgt
     1321 cgtgttccca tacgacgtgg agaaagagtt cgggacgagc ggtaaggtgc ccgtcgacgc
     1381 gacgatcgac ggcgagccgg agaagagctc gttgatgcgg atggggatgc cgcagcacat
     1441 tctcggcgtg gcgaaagcga tccgagaaaa gatcggcaag aagccaggca acgtcgtgac
     1501 gatcgtgctg tggaaggacg acgcgccgcg cgaagtcgcc gtaccgccgg agctgcaagc
     1561 gcgaatgaag aaagcgggcg tcttggcgtt tttcgagagc ttgagcttca ctcaccgcaa
     1621 ggaatactgc cgttggatca ccgaagccaa gaaggaagag acacgcgcgc ggcggctcgc
     1681 tagggcgatc gagctgctga cggacggagt ccgcactccg ggctagaact tctccgacca
     1741 cgggcgcaga tcgagctcct gcgtccaggc gctgcggtgc tgccgatgca gcgcaacgta
     1801 ctgcgccgcg atcgcgtcgg gatcgagcag cgaatcgggt ccacgctcgc cttccaggcg
     1861 cgcgtagcgc tgcgagcgaa tttgcccgtc gatgatcacg tgcgcgacgt ggatgttctt
     1921 cggcccgagc tcgcgagcca tgctctgcgc gacggcgcgc aagccgaact tcggcgacgc
     1981 cacgctcacg aagcccgccc cgccgcgcag cgacgcggtc gcgcccgtga acaggatggt
     2041 gccggagccg cgctcgacca tgcggcgcgc cgccgcctgg ccgaccaaga aacccgcgaa
     2101 gcatccgacg cgccaacagc gctcgaactc ggccggatcg gtctcgagga cgctgccgcg
     2161 gacgaacgcg ccggcgttga agaccgcgac ttcagtaggg ccgagcgtcg ccgcttcgtc
     2221 gaaaacgcgc tcgacatcgg cctgcccggt cgcgtcgctg tcgatggcga cgacgcgggc
     2281 cgcttcgttt gcgcgtttca attcggcgag agtcgatgcc gagcgcgccg ccgcgacgac
     2341 cgcgtagccc tcgttcgcga acgcgcgcac gagcgcgg
//
LOCUS       DASSNN010000319         3377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_60305, whole genome shotgun sequence.
ACCESSION   DASSNN010000319 DASSNN010000000
VERSION     DASSNN010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60305"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFL84_05785"
     CDS             <1..202
                     /locus_tag="VFL84_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019649044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HET7608167.1"
                     /translation="QAKVTAPQDSFTTLSVRADAAGQVIIGLPKAGWWGICALNLDDG
                     LQHEGKELSLDAVLWIKAADMK"
     gene            210..914
                     /locus_tag="VFL84_05790"
     CDS             210..914
                     /locus_tag="VFL84_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608168.1"
                     /translation="MTEEVRPAGARLEGWGLLAAFVVVLVMLTLYVRATGREDTREVL
                     YDWQVSAFDGLVGPDQAIYNSLYTVKDEIPIIFDDINAFNEPGQKFRWPNLQDFEDVL
                     LPPFYKDTSWEQNGSVTWSLHEPLAEGEMQGSTMYLGTDGKVPGQGSFLLVIGHVHAG
                     FTNNNAIAIWWNPKNHVEMPQSGFKDQLILAGWREVVPYSGDLEVKRLFGEPSKEPDN
                     GSENVDELLEGIIGDK"
     gene            914..1819
                     /locus_tag="VFL84_05795"
     CDS             914..1819
                     /locus_tag="VFL84_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HET7608169.1"
                     /translation="MLKRTLVRLLALSLAVLGAHAAQAKPLKIGITLHPYYSFVANIV
                     KDRAEIIPLIDADANPHGYAPQPADMIRITSMDVMVVNGIGHDSWAFEILDASGMKDK
                     LKLIYANDGVSLIPIAGDPSGEKVVNPHTFISTTAAIQQVYLIARKLGELDPENAAFF
                     RDNGRKYALEIRKLRAEFDMQIAGVSLAKFRCATMHSGYDYILQELGLQVTAVIEPRH
                     GVEPTARQLAETIDRIKAANVNVLFAEKYFASKLSDTIRDATGVKMYSISHISAGEYT
                     PEKFVDEMRENLATLATAIIESSKS"
     gene            1823..2545
                     /locus_tag="VFL84_05800"
     CDS             1823..2545
                     /locus_tag="VFL84_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET7608170.1"
                     /translation="MQGPSIEFERVNLTLGATTILEDVSFKVRAGTIHCIVGANGGGK
                     TSLVRSLLGQMPHKGSIKIHWRESRVIGYVPQTLDFDKSLPITVGDFMAMATQRRPVF
                     LGVSRARRALIDATLEKLGLPGKRKTRLGSLSGGERQRVLLAQALIPEPALLVLDEPT
                     TGLDVAGREILERTIVDFVKAGGTVIWINHDIAQVGELADALTYIDRKVLLDGAPSEA
                     LSSGIAAHLFPTLTLPMREAVS"
     gene            2545..>3377
                     /locus_tag="VFL84_05805"
     CDS             2545..>3377
                     /locus_tag="VFL84_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018927830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET7608171.1"
                     /translation="MSALYDIPRQFIIGLANDNVIHYTFSYAFVVNGLLCAFVIGPLL
                     GGIGTMVVAKRMAFFSQAVGNAALTGVAIGVIIGESYTAPYMSMFSFCLLFGLLLNFA
                     RNRTKMSADTLIGVFLSISLAIGATALLWVSAKVNTHILDTVMFGSILTVNDTDMSVL
                     LVTTLLTAVTALPLFNQMLLGSLNPSLAHVRGVRVHLLEYIFVLLLTILTVACVKIVG
                     AVLVEALLLIPAAAARNLNKSIRGFVLWSIAFSTISCIAGIYAPMRFDLPLPSGGAII
                     LA"
BASE COUNT          602 a         1093 c         1091 g          591 t
ORIGIN      
        1 gcaagcgaag gtcacggcgc cacaagattc cttcacgacg ctgagcgttc gggccgacgc
       61 ggccggtcaa gtcatcatcg gcttgccgaa ggcgggctgg tggggcatct gtgcgttgaa
      121 tctcgacgac ggcttgcagc acgagggcaa ggagctctcg ctcgacgcag tgctatggat
      181 caaagcggcg gacatgaagt gaggcgggca tgaccgagga agtcagaccc gccggtgcgc
      241 ggctcgaagg ctgggggctg ctcgcggcgt tcgtcgtcgt gctcgtcatg ttgacgttgt
      301 acgttcgcgc gaccggccgc gaggacacgc gcgaggtgct gtacgactgg caggtcagcg
      361 cgttcgacgg cctcgtcggt cccgatcaag cgatttacaa ctcgctctac accgtcaagg
      421 acgagatccc gatcatcttc gacgacatca acgcgttcaa tgagcccggc cagaaatttc
      481 gctggccgaa tctgcaggat ttcgaggacg tgctgctgcc gccgttctac aaggacacga
      541 gctgggagca gaacggctcc gtgacgtggt cgctgcacga gccgctcgcc gaaggcgaga
      601 tgcagggctc gacgatgtat ctcggcaccg acggcaaggt gccggggcag ggctcgttcc
      661 tgctcgtgat cggccacgtg cacgcggggt tcacgaacaa caacgcgatc gcgatctggt
      721 ggaatccgaa gaatcacgtc gagatgccgc agagcggctt caaagatcag ctgattctgg
      781 cgggctggcg cgaggtcgtg ccgtacagcg gcgacctcga ggtcaagcgg ctgttcggcg
      841 agccttcgaa agagcccgac aacggctccg agaacgtcga cgagctgctc gaagggataa
      901 tcggagacaa gtgatgttga agcgcacgct ggtacgtttg ctggccctat ccctcgccgt
      961 gctcggcgcg cacgcggctc aagcgaagcc gctgaagatc ggcatcacgc tgcacccgta
     1021 ctacagcttc gtcgcgaaca tcgtcaagga tcgcgccgag atcatcccgc tgatcgacgc
     1081 cgacgcgaac ccgcacggct atgcgccgca gcccgccgac atgatccgca tcacgtcgat
     1141 ggacgtcatg gtcgtgaacg gcatcggcca cgactcgtgg gcgttcgaga tcctcgacgc
     1201 ctccgggatg aaggacaagc tcaagctgat ctacgcgaac gacggcgtgt cgttgattcc
     1261 gatcgccggg gacccgagcg gcgagaaggt cgtcaatccg cacacgttca tctcgacgac
     1321 ggccgcgatc cagcaggtct atctgatcgc gcgcaagctc ggcgagctcg atcccgagaa
     1381 cgccgcgttc ttccgcgaca acggccgcaa gtacgcgctc gagattcgca agctgcgcgc
     1441 cgagttcgac atgcagatcg cgggggtcag tctcgcgaag ttccgctgcg cgacgatgca
     1501 ctctggctac gactacatct tgcaggagct cgggctacag gtgacggccg tcatcgagcc
     1561 gcgccacggc gtcgagccga cggcgcgcca gctcgccgag acgatcgacc gcatcaaggc
     1621 cgcgaacgtc aacgtgctgt tcgccgagaa atatttcgcg agcaagctgt ccgacacgat
     1681 tcgcgacgcg acgggcgtca agatgtactc gatctcgcac atcagcgccg gcgagtacac
     1741 ccccgagaag ttcgtcgacg agatgcgcga gaacttggcg acgctcgcga cggcgatcat
     1801 cgagtcgagc aagagctagc gcatgcaagg accctcgatc gagttcgagc gcgtcaatct
     1861 gacgctcggt gccacgacga tcctcgagga cgtcagcttc aaggtgcgtg ccggcacgat
     1921 tcactgcatc gtcggcgcga acggcggcgg caagacctcg ctcgtgcgct cgctgctcgg
     1981 gcagatgccg cacaaaggca gcatcaagat ccattggcgc gagagccgcg tcattggcta
     2041 cgtgccgcag acgctcgact tcgacaagag cctgccgatc acggtcggcg acttcatggc
     2101 gatggcgacg cagcggcggc ccgtgttcct cggcgtgtcg cgggcgcgcc gcgcgttgat
     2161 cgacgccacg ctcgagaagc tcggcttgcc cggcaagcgc aagacgaggc tcggcagcct
     2221 gtccgggggc gagcggcagc gcgtgctgct cgctcaggcg ctgatccccg agcccgcgct
     2281 cctcgtgctc gacgagccga ccacgggtct cgacgtggcc ggcagagaga tcctcgagcg
     2341 cacgatcgtc gacttcgtga aggccggcgg caccgtcatc tggatcaacc acgacatcgc
     2401 gcaggtcggc gagctcgccg acgcgctgac gtacatcgac cgcaaggtgc tgctcgacgg
     2461 cgcgccgagc gaggcgctct cgagcggcat cgccgcgcac ctgttcccga cgctcacgtt
     2521 gccgatgcgc gaggcggtgt catgatcagc gcgctctacg acatcccgcg gcagttcatc
     2581 atcgggctcg ccaacgacaa cgtcattcac tacacgttct cgtacgcgtt cgtcgtgaac
     2641 ggcctactct gcgcgttcgt gatcggcccg ctgctcggcg gcatcggcac gatggtcgtc
     2701 gcgaagcgca tggcgttctt ctcgcaggcc gtcggcaacg cggcgctgac cggcgtcgcg
     2761 atcggcgtca tcatcggcga gtcgtatacg gcgccgtaca tgtcgatgtt cagcttctgc
     2821 ctgctgttcg gcctcttgct gaacttcgcg cgcaaccgca ccaagatgtc ggccgacacg
     2881 ctgatcggcg tgtttctgtc gatctcgctc gcgatcggcg cgacggcgct gctctgggtg
     2941 tcggcgaagg tcaacacgca catcctcgac accgtgatgt tcggctcgat cctgaccgtc
     3001 aacgacaccg acatgtcggt gttgctcgtg acgacgctgc tcacggccgt cacggcgctg
     3061 ccgctgttca atcagatgct gctcggcagc ttgaacccga gcctcgcgca cgtgcgcggc
     3121 gtgcgcgtgc acctgctcga gtacattttc gtgctgctgc tgacgatttt gaccgttgca
     3181 tgcgtgaaga tcgtcggcgc cgtgctcgtc gaggcgctgc tgctgattcc ggccgcggcg
     3241 gcgcgcaatc tcaacaagtc gatccgcggc ttcgtgctct ggagcatcgc gttctcgacg
     3301 atcagctgca ttgccggcat ctacgcgccg atgcgcttcg acctgccgct gccatcgggc
     3361 ggcgcgatca tcctcgc
//
LOCUS       DASSNN010000320         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_563103, whole genome shotgun sequence.
ACCESSION   DASSNN010000320 DASSNN010000000
VERSION     DASSNN010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563103"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VFL84_05810"
     CDS             complement(<1..608)
                     /locus_tag="VFL84_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7608172.1"
                     /translation="MKNSGALAAALALAVCAAAQSAIDAQEPAPQTEATTQSPLDGLR
                     RGRDGFIGARDFSAALGPAQSVVDAQSTQREPQYAADLAALGLIQAELRELEPALENL
                     TDAVDIVVKAEGEYSPTLIEYYRALGRTYIKTGQYQQAIVTLEQAQHITQRHLGLFNV
                     EQAALLDDITTAYLGLGNTVDAQRMQVERLDNAVRRFGAADPR"
     gene            complement(598..1962)
                     /locus_tag="VFL84_05815"
     CDS             complement(598..1962)
                     /locus_tag="VFL84_05815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET7608173.1"
                     /translation="MRSATPNSRWCSRGRTWLLAWLMCGAIASAPSAQTDAGATEPAA
                     QPRALDDAVAQAPDEAPADAPDLVPAEPVDPVAAGERLLKSNIAEFGADSVQAAEAHL
                     DLANAQRAAKQFDKAAESYLAAVEAYRSVDGPFTPLAIGPLTSLGDNYHEANDDANAV
                     SAYSEARTVSRRAYGLHNTQQVELLDRMSRSLLDLNQLSEAEAQQVEALRLVQRAYPP
                     GSDEVLAAIYKYAEWLGDRLFYQLQRDQYARALRIIRQTYGDNDVRQVTPLLGIGNAY
                     REERNPASMGISALEEALNLLQEQPERDPVATAMALRDMGDWAVAFGKTGYSGTEYQR
                     AWQMLGSAPNGDALRRQWFTGANYVLYEPISPRGLSTDPDALSGHVTVSFDIDTAGNT
                     NNVMLVESEPAGLKDEAVLRHIRRSRFRPVIENGNVVVGKSLAIQVKFRYLPEAAAGG
                     DDEK"
     gene            2135..>3241
                     /locus_tag="VFL84_05820"
     CDS             2135..>3241
                     /locus_tag="VFL84_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011642290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7608174.1"
                     /translation="MDPTDTRRNEYFHRVVDCQWACPAHTDVPEYIRLVAQGRFGDAY
                     LLNRQSNVFPGILGRVCDRPCEPACRRGRVEEKPVAICRLKRVAADYRGDVESRLPRP
                     PQHKNGRRVALVGAGCASLTVANDLAPLGYECVIFEAMDRLGGLMRTNIPAFRLPAEV
                     LDEEIAYVTRLGVEVKTNSPVASLRALLDDGYDAVFVGSGAPKGKNLVLPGRDASERI
                     HIGIDWLGSIAFGHTDSIGERVLIIGVGNTAMDCCRSSLRLGAKDVKVIARKPRQFFK
                     ASDWELEDAEEERVEIVVNLSPKEFVIENGKLVGMRFDELAYELDGRGEITSTKTLRE
                     VLLPCDDVVLAIGQENAFPWIERDLGIDFDRWSVP"
BASE COUNT          489 a         1061 c         1112 g          579 t
ORIGIN      
        1 cgcggatccg cggcgccgaa ccgccggacc gcgttgtcga gccgctcgac ctgcatccgc
       61 tgcgcgtcga cggtgttgcc caaccccaag tacgccgtcg tgatgtcgtc gagcagcgca
      121 gcctgctcga cgttgaacaa accgagatgg cgctgcgtga tgtgctgcgc ttgctcgagc
      181 gtgacgatcg cctgctggta ctggccggtc ttgatgtagg tgcgcccgag cgcgcggtaa
      241 tactcgatca gcgtcggcga gtactcgccc tcggctttga cgacgatgtc gacggcgtcg
      301 gtgaggttct cgagcgccgg ctcgagctcg cgcagctccg cttgaatcaa gccgagcgcg
      361 gcgaggtccg ccgcgtactg cggctcccgt tgcgtgctct gtgcgtcgac gacgctttgc
      421 gcgggcccga gcgccgcgct gaagtcgcgt gcgccgatga acccatcgcg gccgcggcgc
      481 aagccgtcga gcggcgattg cgtcgttgct tctgtttgcg gagcgggctc ttgcgcgtcg
      541 atcgcgcttt gcgccgccgc gcatacggcg agcgcgagcg cggcggcaag cgctccgcta
      601 tttttcatcg tcgccgcccg cggctgcttc gggcagatag cggaacttga cttgaatggc
      661 gaggctcttg ccgacgacga cgttgccgtt ctcgatgact gggcggaaac gcgagcggcg
      721 gatgtggcgc agcaccgctt cgtccttgag gccggcgggc tcggattcga cgagcatcac
      781 gttgttcgtg ttgcctgccg tgtcgatgtc gaagctgacc gtgacgtggc cgctcaacgc
      841 gtcgggatcc gtgctcaagc cgcgcgggct gatcggctcg tacaacacgt agttcgcgcc
      901 cgtgaaccac tggcgccgca gcgcgtcgcc gttcggcgcc gagccgagca tttgccacgc
      961 gcgctggtac tccgtgcccg agtagccggt cttgccgaac gcgacggccc aatcgcccat
     1021 gtcgcgcagc gccatcgcag tcgcgaccgg gtcgcgctcg ggctgctcct gcaacaggtt
     1081 cagcgcctcc tcgagcgccg agatgcccat gctcgcggga ttgcgttctt cgcgatacgc
     1141 gttgccgatt ccgagcaacg gcgtcacttg cctgacgtcg ttgtcgccgt aggtctggcg
     1201 aatgatgcgc agagcccgag cgtactggtc gcgttgcagc tgatagaaca accggtcgcc
     1261 gagccactcc gcatatttat agatggcggc gagcacttcg tccgagccgg gcggataggc
     1321 gcgctgcacg agccgcagcg cttcgacttg ctgggcctcc gcttcgctca actgattcag
     1381 gtcgagcagc gagcgcgaca tgcgatcgag cagctcgacc tgctgcgtgt tgtgcaggcc
     1441 gtacgcgcgc cggctgaccg tgcgcgcttc ggaatacgcc gagacggcgt tcgcgtcgtc
     1501 gttggcttcg tgataattgt cgccgaggct cgtcaacggc ccgatcgcga gcggcgtgaa
     1561 cggcccgtcc acggagcgat acgcctcgac ggccgcgaga tagctttccg cggctttgtc
     1621 gaactgcttc gccgcgcgct gcgcgtttgc gagatcgagg tgcgcttcgg cggcttgaac
     1681 gctgtccgcg ccgaactccg cgatgttgct cttgagcagg cgctcgcccg ctgcgacagg
     1741 atcgacgggc tcggcgggga cgagatcggg cgcgtcggcc ggcgcctcgt ccggcgcttg
     1801 cgccacggcg tcgtcgagcg cgcgcggctg cgcggcaggc tccgttgcgc ccgcgtcggt
     1861 ttgcgcggac ggcgcggagg caatcgcgcc gcacatgagc cacgcgagca gccacgtgcg
     1921 gccgcggctg caccaacgag aattgggcgt ggcggacctc atcgctggca ttctatcccc
     1981 aataaaaggc ggccgccgaa gcctccgcgg caacgagttg agtccccacc gaaatcggta
     2041 tcgttcagtg tgtgcagcgc tcgccaagtg cctataatgg cgcgccccgc aatgcccatc
     2101 cgaggttgtt gctgacgttt ccgacgttgc ctgaatggat ccgaccgata cccgaaggaa
     2161 cgagtacttt caccgcgtag tcgattgcca atgggcgtgc ccggcgcaca cggacgtgcc
     2221 cgagtacatt cggctcgtgg cgcagggccg attcggcgac gcttatttac tcaaccggca
     2281 atcgaacgtt ttccccggaa tcttgggccg cgtctgcgac cggccgtgcg agcccgcttg
     2341 ccggcgcggg cgcgtcgagg agaagccggt cgcgatctgc cgcttgaagc gcgtcgcggc
     2401 ggattatcgc ggcgacgtcg agagccgcct gccgcgcccg ccgcaacata agaacggccg
     2461 ccgcgtcgcg ctcgtcggcg cgggctgcgc gtcgctgacg gttgcgaacg acttggcgcc
     2521 gctcggctac gagtgcgtga tcttcgaggc catggatcgc ttgggcggcc tcatgcgcac
     2581 gaacataccg gcgttccgtt tgccggccga ggtgctcgac gaagagatcg cttatgtcac
     2641 gcggctcggc gtcgaggtca agacgaattc tccggtcgcg agcctgcgcg cgctgctcga
     2701 cgacggctac gacgccgtgt tcgtcggcag cggcgcgccg aaaggcaaga acctcgtgct
     2761 gcccggccgc gacgcgagcg agcgcattca tatcggcatc gattggctcg gctcgatcgc
     2821 gttcggccac acggactcga tcggcgagcg cgtgctgatc atcggcgtcg gcaacacggc
     2881 gatggactgc tgccgcagct cgctgcggct cggcgcgaag gacgtcaagg tcatcgcgcg
     2941 caagccgcgg cagttcttca aagcttcgga ctgggagctc gaggacgccg aggaagagcg
     3001 cgtcgagatc gtcgtgaact tgagcccgaa agaattcgtg atcgagaacg gcaagctcgt
     3061 cggcatgcgt ttcgacgagc tcgcgtacga gctcgacggc cgcggcgaga tcacgagcac
     3121 gaagacgttg cgcgaggtgc tcttgccgtg cgacgacgtc gtgctcgcga tcggtcagga
     3181 gaatgcgttc ccctggatcg agcgcgacct cggcatcgac ttcgaccgct ggagcgtccc
     3241 g
//
LOCUS       DASSNN010000321         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1680666, whole genome shotgun sequence.
ACCESSION   DASSNN010000321 DASSNN010000000
VERSION     DASSNN010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680666"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /gene="queC"
                     /locus_tag="VFL84_05825"
     CDS             complement(<1..535)
                     /gene="queC"
                     /locus_tag="VFL84_05825"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HET7608175.1"
                     /translation="MTRSVVLLSGGLDSATALAIARSRGDECHTLSFDYGQRHRSELA
                     AARRISASLHAAAHREMQVSLDVFGGSALTDKSIAVPEHASTGIPVTYVPARNTVFLS
                     LAMALAEVIDAGKIVIGVNFLDSSGYPDCRPEFIAAFQQLARLATKRGVEGGALTIDA
                     PLITLSKAAIIRYGSDLG"
     gene            complement(538..1206)
                     /gene="queE"
                     /locus_tag="VFL84_05830"
     CDS             complement(538..1206)
                     /gene="queE"
                     /locus_tag="VFL84_05830"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019302930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HET7608176.1"
                     /translation="MAAAAEREPAAARRLKVTEIFFSIQGEAVFSGWPTVFVRLTGCP
                     LRCQYCDTAYAFTGGEWRTFEEIETEVRRHATPYVCVTGGEPLAQPRCAALLKELCEH
                     GFNVSLETSGAIDISDVDTRVARVVDLKTPGSKEVERNRWQNLALLTPRDAVKIVICD
                     RADYEWARDVVAKHPVPCPVFFSPSYEELAAGTLADWILEDRLPVRLQVQLHKVLWGN
                     VPGR"
     gene            complement(1216..>2004)
                     /gene="ybgF"
                     /locus_tag="VFL84_05835"
     CDS             complement(1216..>2004)
                     /gene="ybgF"
                     /locus_tag="VFL84_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HET7608177.1"
                     /translation="THVKRLHLAVGVAVVASGCSSLSPTEDPVALRMQDLEARLIRIE
                     RVVENQSLVELSNELERLRSETTALRGEIESLRFETENSDSRQRELYVDVDERLRTLE
                     AAPRGFEPPPVAPQVDGAPAATASNPAVTVPARPAGSDQQNYQAAFDLISARRYEEAG
                     AGFERFLVSFPTSPLADNAQYWLAETHYVRGQYAEALPAFRKVLEKYPQSAKLPDALL
                     KVGYCQIELGDRNAARTSLQEVMRQFPDTTAARLASQRLSSLPR"
BASE COUNT          321 a          684 c          686 g          313 t
ORIGIN      
        1 cgccgaggtc gctgccgtaa cggatgatcg cggctttcga cagcgtgatc agcggcgcgt
       61 cgatcgtcag cgcgccgcct tcgacgccgc gcttcgtcgc gagccgcgcg agctgctgga
      121 aggcggcgat gaactccggc cggcaatcag gatagccgga gctatcgagg aagttgacgc
      181 cgatgacgat cttgccggcg tcgatgacct cggcgagcgc catcgccaag ctcagaaaca
      241 cggtgttgcg cgcagggacg taggtcaccg gaattcccgt gctggcgtgc tccggcaccg
      301 cgatgctctt gtcggtcagc gccgagccgc cgaacacgtc gagcgaaacc tgcatctcgc
      361 gatgcgccgc agcatgcaaa gacgcggaga tgcgccgcgc ggccgcgagc tcggaccggt
      421 gccgctgacc gtaatcgaac gacagcgtgt ggcactcgtc gccgcgcgaa cgcgcgatcg
      481 cgagcgccgt cgccgaatcg aggccgcccg agagcagcac gacggagcgc gtcatcgtca
      541 gcgtcccggc acgttgcccc agagcacttt gtgcagctgt acctgcaagc gcacgggtaa
      601 acggtcctcg agaatccaat cggcgagcgt gccggcggca agctcctcgt agctcggcga
      661 gaagaacacg gggcacggca ccggatgttt cgcgacgacg tcgcgcgccc actcgtaatc
      721 ggcgcggtcg cagatcacga tcttgacggc gtcgcgcgga gtcagcagcg cgagattctg
      781 ccagcgattg cgctcgacct ctttcgagcc cggcgtcttc aagtcgacga cgcgcgcgac
      841 gcgcgtatcg acgtccgaga tgtcgatcgc gccgctcgtc tcgagcgaga cgttgaagcc
      901 gtgctcgcag agctccttaa gcagcgccgc gcagcgcggc tgggcgagcg gctcgccgcc
      961 cgtcacgcag acgtacggcg tggcgtggcg gcggacttcc gtttcgatct cctcgaacgt
     1021 gcgccactcc ccgcccgtga acgcgtaagc cgtgtcgcaa tactggcagc gcagcgggca
     1081 gcccgtgagc cgcacgaaca cggtgggcca accgctgaac acggcctcgc cctggatcga
     1141 gaagaaaatc tccgtgacct tgagccgccg cgccgcagca ggctcgcgtt ccgccgctgc
     1201 ggccacggga attctctagc gcggcaacga agagagccgt tgcgacgcga gcctcgcggc
     1261 cgtcgtgtcg gggaactgcc gcatgacttc ctgcagcgac gtgcgcgcgg cgttgcggtc
     1321 gccgagctca atctggcagt agccgacctt gagcagcgcg tcgggcagct tcgccgactg
     1381 cggatacttc tcgagcacct tgcggaatgc cggcagcgct tcggcgtact ggccgcgcac
     1441 gtagtgcgtc tcggcgagcc agtattgcgc gttgtccgcg agcgggctcg tcgggaagct
     1501 cacgaggaac cgctcgaacc cggcgcccgc ttcctcgtag cgacgcgccg agatcaggtc
     1561 gaacgccgcc tggtaattct gctggtcgct gcccgcgggc cgcgccggta ccgtgacggc
     1621 ggggttcgac gcggtcgcag cgggcgcgcc gtcgacctgc ggcgcgaccg gcggcggctc
     1681 gaagccgcgc ggcgcggcct cgagcgttcg caaccgctcg tcgacgtcga cgtagagctc
     1741 acgctggcgg ctgtcgctgt tctcggtttc gaagcgcagc gactcgatct ccccgcgcag
     1801 cgcggtcgtc tcgctgcgta agcgctcgag ctcgttcgag agctcgacca ggctctggtt
     1861 ctcgacgacg cgctcgatgc ggatcagccg cgcttcgaga tcctgcatgc gaagcgcaac
     1921 gggatcctcg gtcggcgaaa gcgagctgca gccgctcgcg acgaccgcta cgcccacggc
     1981 taggtggagt cttttcacgt gcgt
//
LOCUS       DASSNN010000322         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_189019, whole genome shotgun sequence.
ACCESSION   DASSNN010000322 DASSNN010000000
VERSION     DASSNN010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189019"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFL84_05840"
     CDS             complement(<1..445)
                     /locus_tag="VFL84_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HET7608178.1"
                     /translation="MSTPCIITVAITGSVPTKKDSPAVPISVAEQIESSHAAYEAGAS
                     LVHVHVRNDDGTTTSDPERFARFLAGIRRHCPAMIVQFSTGGRSGTGKERGGMLHLKP
                     EIEIFDLAMLYNAAKLVADGLLKAPVHVQFVLGIPGALPADREVLE"
     gene            524..958
                     /locus_tag="VFL84_05845"
     CDS             524..958
                     /locus_tag="VFL84_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET7608179.1"
                     /translation="MPTLTLAQANQIVTKALEKARAMKIKPVTVVVLDDAGHLKAMQR
                     EDGASMFRFDVATGKAWAAVGMGASSRALAGRAKDNPNFFITLAATAGGKFLPQIGGV
                     LIRDSSGAILGAAGASGGTGEEDEAVCAHGIEHAGLVADAKP"
     gene            complement(955..2292)
                     /locus_tag="VFL84_05850"
     CDS             complement(955..2292)
                     /locus_tag="VFL84_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7608180.1"
                     /translation="MKKVWQIAGREFVATVFTKAFLIGLLIVPLIGIVLSLVAPRLFG
                     ARDLTIEGEIVVIDPTGVVLPEVQAAISARGSPVRVGEIVERARAGGADQMVLSVLGA
                     STRLTLVERPSSDDVEQQKRWLLEPTPAAKHLALVVVHSNAVLPAPGTSNLGTYDLYV
                     PPRQDNRVEVAVQNVMREAIIEARVFARDLNREELTLVTTVPRVRSVTVGADGERNTV
                     VGFNLVIPVVFMGLLIMGVMFAGQGMLTTTVEEKSNRVMEVLLSAVSPMQLMGGKLIA
                     HMGVSLLAMSLYLVFGLVALASFSLFGLLDFKLILYLLVFYLIAFFTIGSLMMACGAA
                     VNDMREAQQLMMPLMMTMIVPWMLWWPIVQNPNSTLAVVVSLVPPVSSFGMLLRVASS
                     SPPPLWQVWLSIGLGVAGVFGAVWFAAKVFRVGILMFGKAPNFKTLIAWVRAA"
     gene            complement(2289..>2667)
                     /locus_tag="VFL84_05855"
     CDS             complement(2289..>2667)
                     /locus_tag="VFL84_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608181.1"
                     /translation="PQAEEICDHVVMIHRGQKVLDDPVRTILRPHDSRALTFEPLDAH
                     IDLSAVRALPEVERCIVADGRYEVALRDGVDAAAAMGRVLGVVPAARIELKRRSLEDV
                     FVSIVAGSQGSAGTAGGANVGVA"
BASE COUNT          494 a          907 c          870 g          396 t
ORIGIN      
        1 actcgagcac ttcgcggtcg gccggcagcg cgcccgggat gccgagcacg aactgcacgt
       61 gcacgggcgc tttcagcaac ccgtcggcca cgagcttcgc cgcgttgtag agcatcgcga
      121 ggtcgaagat ctcgatctcc ggcttcagat gcagcatgcc gccgcgctct tttccggtgc
      181 cgctgcgccc accggtcgag aactgcacga tcatggccgg gcaatgccgg cggatgccgg
      241 ccaggaaccg ggcgaaccgt tcgggatcgg acgtcgtcgt gccgtcgtcg ttgcggacgt
      301 ggacgtgaac cagcgaggca ccggcctcat acgccgcgtg cgacgactcg atttgctcgg
      361 cgacggagat cgggaccgcc gggctgtcct tcttcgtcgg caccgagccc gtgatggcga
      421 cggtgatgat gcacggcgtg ctcatgaggg cgatctctct ttccggggtt ggcggctcag
      481 cgccgacatt ctagacttga atcgtccccc gcggagtaga cgaatgccca cattgacgct
      541 tgcccaggcg aatcagatcg tgaccaaagc gctcgaaaag gcgcgtgcca tgaagatcaa
      601 gcccgtcacg gtcgtcgtgc tcgacgacgc cgggcacttg aaggcgatgc agcgcgagga
      661 cggcgcgagc atgttccgct tcgacgtcgc gaccggcaag gcgtgggccg ccgtcggcat
      721 gggcgcgtcg agccgcgcgc tcgcgggccg cgccaaggac aacccgaatt tcttcatcac
      781 gctcgcggcg accgcgggcg gcaagttcct gccgcagatc ggcggcgtgc tgatccgcga
      841 ttcgagcggc gcgattctcg gcgccgccgg cgcgagcggc ggcacgggcg aggaagacga
      901 agccgtctgc gcgcacggca tcgagcacgc cgggctcgtc gccgacgcga agccttaggc
      961 cgcgcgcacc cacgcgatca gcgtcttgaa gttcggcgcc ttgccgaaca tcaggatgcc
     1021 gacgcgaaat accttggccg cgaaccacac ggcgccgaag acgccggcga cgccgaggcc
     1081 gatcgacagc cacacctgcc acagcggcgg cgggctcgag gacgcgactc gcagcagcat
     1141 gccgaacgag ctcaccggcg gcaccaggct cacgacgacg gcgagtgtcg aattcgggtt
     1201 ctgcacaatc ggccaccaca gcatccacgg cacgatcatc gtcatcatca gcggcatcat
     1261 gagctgctgc gcctcgcgca tgtcgttgac ggcggcacca caggccatca tcaacgagcc
     1321 gattgtgaag aacgcgatga gatagaagac cagcagatac aaaatcagct tgaagtcgag
     1381 caaaccgaac aacgagaacg acgcgagcgc cacgagaccg aagacgagat acaagctcat
     1441 cgcgagcaga ctgactccca tgtgcgcaat cagcttgccg cccatcagct gcatcggcga
     1501 tacggccgac agaagcacct ccatgactcg attcgatttc tcctcgaccg tcgtcgtgag
     1561 catgccttgg cccgcgaaca tcacgcccat gatgagcagg cccatgaata cgacggggat
     1621 gacgaggttg aatccgacca cggtattgcg ttcgccgtcg gcgccgaccg tgacggagcg
     1681 gacgcgcggc acggtcgtga cgagcgtcag ctcctcgcgg ttcaaatccc gcgcaaagac
     1741 gcgcgcctcg atgatcgctt cgcgcatgac gttctgcacg gcgacctcga cgcgattgtc
     1801 ctgccgcggc ggcacgtaga gatcgtaggt tccgagattc gacgtgcccg gagcgggcaa
     1861 cacggcgttc gagtgcacca cgacgagcgc gagatgcttc gcggcaggcg tcggttcgag
     1921 tagccagcgc ttctgctgct cgacgtcgtc gctcgaaggc ctctccacga gcgtcagccg
     1981 cgtcgacgcg ccgagcacgc tcaggaccat ctggtccgcc ccgcctgcac gcgcgcgctc
     2041 gacgatctcg ccgacccgta cgggcgagcc cctcgcggag atggcggcct gcacctcggg
     2101 cagcacgaca ccggtcgggt cgatgaccac gatctcgccc tcgatcgtca ggtcgcgagc
     2161 gccgaaaaga cgcggggcga cgagcgacag cacgatgccg atcagcggca cgatcagcaa
     2221 cccgatgagg aatgctttcg tgaacaccgt ggcgacgaac tcgcgtccgg cgatttgcca
     2281 gaccttcttc atgcgacccc cacgttcgcg ccgcccgccg tgccggcgga gccttgcgag
     2341 cccgcgacga tcgagacgaa cacgtcctcg aggctacgcc gcttcagctc gatgcgcgca
     2401 gccggcacga cgccgagcac gcggcccatc gcggcggccg cgtcgacgcc gtcgcgcaac
     2461 gcgacctcgt agcggccgtc ggcgacgatg cagcgctcga cctccggcag cgcgcgcacg
     2521 gcgctcaagt cgatgtgcgc atcgagcggc tcgaacgtca gcgcgcgcga atcgtgcggc
     2581 cggaggatcg tgcgcacggg atcgtcgagc actttttgtc cgcgatgaat catgacgacg
     2641 tggtcgcaga tctcttcggc ttgcggc
//
LOCUS       DASSNN010000323         6879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_800641, whole genome shotgun sequence.
ACCESSION   DASSNN010000323 DASSNN010000000
VERSION     DASSNN010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800641"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..530)
                     /locus_tag="VFL84_05860"
     CDS             complement(51..530)
                     /locus_tag="VFL84_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7608182.1"
                     /translation="MKKLVATLLGASLVSVLTACGGGGGAEIPDTPEGAAFQYRHGVM
                     EAVSYKVARLRAMAMGEIPADDAAFAKDARDVAALAGMIPEGFIPNSIVGPSIAKPEI
                     WTNKADFDAKAMEFQKNAEALAMTASTQGFEAAKGMVQAVGGACGGCHRAYRASRAE"
     gene            complement(617..1027)
                     /locus_tag="VFL84_05865"
     CDS             complement(617..1027)
                     /locus_tag="VFL84_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608183.1"
                     /translation="MMTSDDLKERIEAIESGYEFMLAYAAQGRQTDKGAAAGRNVREY
                     LDKMVAALEGLGPVVDACAKGVDPELAAKGAAFFAAVDTDARVALAALELVLAREDIS
                     SQLIDNMNASIHLRALLTDLFVVDEVLKPSKTHG"
     gene            complement(1024..1689)
                     /locus_tag="VFL84_05870"
     CDS             complement(1024..1689)
                     /locus_tag="VFL84_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET7608184.1"
                     /translation="MTTPTRTSTGGSATADRIVRHAPADRLLHWAIAACVLTLLGTAF
                     LPIVGVEFGWVTIHWVTGLVLGVAVLLHVVRVLVRGTLSSMWIGRADVADALDVASSA
                     LRRSPPTRRPGKYSVAQKLIHHAFTAVVLTTLVTGSLMLLRIDTPWWKRNPYLLADST
                     WGIVYVLHGLAALLLITMVMLHVYFALRPEKLMFTRAMIVGWITRRELAEHHDPERWQ
                     VDR"
     gene            complement(1697..1849)
                     /locus_tag="VFL84_05875"
     CDS             complement(1697..1849)
                     /locus_tag="VFL84_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608185.1"
                     /translation="MELFRVSRDIYGREVLQGMSWDLLWVFVGAGLVLIVAHAAYRLL
                     LAPKPK"
     gene            complement(1862..2866)
                     /locus_tag="VFL84_05880"
     CDS             complement(1862..2866)
                     /locus_tag="VFL84_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009109130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET7608186.1"
                     /translation="MSRDMMSAFELYQPDTVEGAVDLLGRFGEEGWALAGGNDSFDWF
                     KDRIKKPKYVVDLGGIAALKGIRETQDGGVEIGALTTLTEVERNSVIQSRYKVLSDAA
                     RRVASPQIRNSGTIGGNACQDARCWYYRYGVNCYRAGGNTCYADTPEGQNREHCLWGA
                     DRCVAVSPSDTAPALVALDATMVIVGKGGQREVKAEEFFIGPNIDITRMTVLEDGDVL
                     TAIRLPADWSGARFYFEKVADRNTWDFPLVNIAAAMKVSGAAGSEKIDDIRIAAGAVQ
                     CVPRRLTVVEQVVRGQAKTADTASLAGQSAVRGATPLNFNHFKIPLLQNLVTRAIRDA
                     "
     gene            complement(2902..3564)
                     /locus_tag="VFL84_05885"
     CDS             complement(2902..3564)
                     /locus_tag="VFL84_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7608187.1"
                     /translation="MADNTNGRSYPEPRFDSFISKATSRRQFIKGVIFSGAAVTGGGY
                     LLSLAGCSSGGGSAAGVERLLTLNVNGQMRPVDVAPNETLAMTLRYKLGLTGTKLGCD
                     RGECGACTVLIDGVASYSCSTLTHAVRGRAVLTIEGLEGPNGELHKVQQAMIDELGPQ
                     CGFCTPGQIMSAVALLNENPTPTRDEVRHALSGNLCRCGAYDHYLNAVMRAATGDRVS
                     QA"
     gene            complement(3667..6066)
                     /locus_tag="VFL84_05890"
     CDS             complement(3667..6066)
                     /locus_tag="VFL84_05890"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HET7608188.1"
                     /translation="MAFDLLGKNFTPPDVHAKVTGKAKYSEDIRAEGMVFVKLLTSPM
                     PHARVTNVDDKAALAMPGVHGILRASEVSNPPDPQETILTDEPLFVGHPILAIAADTE
                     ELASEALEKIEVTYEELPFTVDPLQSLYPGGPNARTNGNSVQGPELRSVKWTAQDFAA
                     VQEGQLPQGAPTAEWSFGDTDAGFARAKVVLDETFVTAGLAHHSMEPRSALSFWQNGK
                     CYLYGSTQSQSFPFPTIARYIGVEPENLVYVSEFCGGGFGSKGGAYPLMAIPALMAKK
                     IGRPVMMRVSRHEEYFMGSARAGFQGRIKMGFAADGRVTAVDLYIVQECGPNQGFTDW
                     LSAADAVSLVYQPPAMRFRGVSVMTNTPPRGPQRGPGQNQIAAAVEPLIDKAAKQLGV
                     DRVAIRRINAPDNAAKYGRMQGGVTSAYLKNALDRGAELFGWAERQAGSGQVNGSKVR
                     AVGVGTAFHPAGNSGFDGLVRITPDGKIHIHTGVGNLGTFSHTATSRVAAEVLKANWD
                     NCEVVRGDSSKHLPWNLGQFGSNTSFTMTRTNYAAAMDAVAKLKEIAAMDLGGAPDDY
                     DIGGERVFAKSDSSKGLTYAQAAQRAIELGGKFDGHEAPDGLNAMTKASVAGVAGTGL
                     VGVAKDNIPLTGTVPALAAGFIEIELDKETGKFDILDYVGVADCGTVLHPQGLDHQIK
                     SGAVMGFGLAASERHIYDPQNGLPGNVGLLQAKPVTYLDVPATMRTASVDIADAQNPV
                     GAKGIGEPVQGCAAAALLCAISDALGGHYFNRTPVMPDMIVNAAAKREPSHKPLQVNT
                     A"
     gene            6173..6667
                     /locus_tag="VFL84_05895"
     CDS             6173..6667
                     /locus_tag="VFL84_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608189.1"
                     /translation="MDVGTWELLSYVVTTIGLPLAILVFIYEQKKERDNEDEEVYQLL
                     SDNYQDFLRMALGHPDLHLFTPEQTPELNDEQRERMLIIFNMLISLFERAYLLLYEEK
                     MSSKHKRRWLSWEDYMREWCRRDDFRALLPVLLEGEDPEFGAYIRALAEDVSPTGSLL
                     LSSS"
     gene            complement(6675..>6879)
                     /locus_tag="VFL84_05900"
     CDS             complement(6675..>6879)
                     /locus_tag="VFL84_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608190.1"
                     /translation="RVSEDVIEYEVTVTDPKVYTAPWTVKLPLNRDPRYVMFEYACHE
                     GNLALPNALSAGRARERAATSAR"
BASE COUNT         1102 a         2354 c         2217 g         1206 t
ORIGIN      
        1 agatctattt tccgcgcttt tggcggaaaa tagatctgtc cccttattgc ctactccgcg
       61 cggctcgcgc gatacgcccg gtggcagccg ccgcaagcgc cgccgacggc ctgcaccatg
      121 cccttcgcgg cttcgaagcc ttgcgtcgac gcggtcattg ccagcgcctc cgcgttcttc
      181 tggaactcca tggccttggc atcgaagtcg gccttgttgg tccagatctc gggcttcgcg
      241 atgctgggcc cgacgatgct gttcgggatg aagccctcgg gaatcatgcc ggcgagcgcg
      301 gcgacatctc tcgcgtcctt cgcgaacgcg gcgtcatccg ccgggatctc gcccatcgcc
      361 atggcgcgca gccgcgccac tttgtaagac acggcttcca tgaccccatg gcgatattgg
      421 aacgccgcgc cttccggcgt atccggaatt tcggcgccac cgccgccacc gcacgcagtg
      481 agaacggaaa cgagcgaagc accgagcaac gtggcgacga gttttttcat gacagatcct
      541 tcgtggcttg cgcaaacatc gagccagcat agccaaagcg cgcgcgcggc cgcaagaccg
      601 aagcggttcg ccgtcgtcag ccgtgcgtct tcgaaggctt cagcacctcg tcgacgacga
      661 acagatccgt caagagcgcg cgcaagtgga tcgacgcgtt catgttgtcg atgagctgcg
      721 agctgatgtc ctcgcgcgcg agcacgagct cgagcgccgc gagcgcgacg cgcgcgtccg
      781 tgtcgacggc ggcgaaaaac gccgcgccct tcgccgcgag ctcgggatcg actcccttgg
      841 cgcacgcgtc gacgacgggc cccaacccct cgagcgccgc gaccattttg tccaagtact
      901 cgcgaacgtt gcggccggca gcggcgcctt tgtcggtctg acggccctgc gccgcatacg
      961 cgagcatgaa ctcgtacccg ctctcgatcg cctcgatacg ctctttgaga tcgtcgctcg
     1021 tcatcatcgg tctacctgcc aacgttcggg atcgtggtgc tcggcgagct cgcgccgcgt
     1081 gatccagccg acgatcatcg cgcgcgtgaa catgagcttc tcgggccgca gcgcgaaata
     1141 aacgtgcagc atcaccatcg tgatgagcag cagcgccgcg agcccgtgca acacgtacac
     1201 gataccccac gtcgagtcgg caagtagata aggattgcgt ttccaccacg gcgtatcgat
     1261 gcgcagcagc atcagggagc cggtcacgag cgtcgtcagc acgacggccg tgaacgcgtg
     1321 gtggatgagc ttctgggcca ccgagtattt gccgggcctt cgcgtcggcg gcgaacggcg
     1381 cagcgccgag ctggcgacgt cgagcgcgtc ggcgacgtcg gcgcgcccga tccacatcga
     1441 gctcaaggtg ccgcgcacga gcacgcgcac gacgtgcagc aggaccgcga cgccgagcac
     1501 gagccccgtg acccagtgga tcgtgaccca gccgaactcg acgccgacga tcggcaggaa
     1561 cgccgtgccg agcagcgtca gcacgcacgc cgcgatggcc caatgcagca ggcgatcggc
     1621 cggcgcgtgc cggacgatac ggtcggccgt cgccgagccg ccggtgctcg tgcgtgtggg
     1681 cgtcgtcacg ggttcgctat tttggcttcg gcgcgagcag cagccgatag gcggcgtgag
     1741 ccacgatcaa gacgaggccg gcaccgacga acacccagag gagatcccac gacatgcctt
     1801 gcagcacttc gcggccgtag atgtcgcggc tgactcgaaa gagctccacg agccttaagc
     1861 ttcaggcgtc gcggatcgcc cgagtcacga gattctgcag cagcggaatc ttgaagtggt
     1921 tgaagttgag cggcgtcgcg ccgcgcacgg cactttgccc ggcgagcgac gccgtatcgg
     1981 ccgtcttcgc ctggccgcgc acgacctgct ccacgacggt gagccgccgc ggcacgcact
     2041 gcacggcgcc ggccgcgatt cggatgtcgt cgatcttctc ggagcccgcc gcgccgctga
     2101 ccttcatcgc cgccgcgatg ttgacgagcg ggaaatccca cgtgttgcgg tccgcgacct
     2161 tctcgaaata gaagcgcgcc ccgctccagt cggcaggcag ccgaatcgcc gtcagcacgt
     2221 cgccgtcttc gagcacggtc atgcgcgtga tgtcgatgtt cgggccgatg aagaattcct
     2281 cggccttgac ctcgcgctgg ccgcccttgc cgacgatgac catcgtcgca tcgagcgcga
     2341 cgagcgcggg cgcggtgtcc gaggggctca ccgcgacgca gcggtccgct ccccagaggc
     2401 agtgctcgcg gttctggccc tcgggcgtgt cggcgtagca ggtgttgccg cctgcgcgat
     2461 agcagttgac gccgtagcgg tagtaccagc agcgcgcgtc ttggcatgcg ttgccgccga
     2521 tcgtgcccga attcctgatc tgcgggctcg cgacgcggcg cgccgcgtcg gacagcacct
     2581 tgtaacggct ctgaatgacc gagttgcgct cgacttccgt caacgtcgtc agcgcgccga
     2641 tctcgacgcc gccgtcttgc gtctcgcgaa tgcctttcag cgcggcgatg ccgcccaagt
     2701 cgacgacgta cttcggcttc ttgatgcggt ccttgaacca gtcgaagctg tcgttgccgc
     2761 cggccagcgc ccatccttct tcgccgaatc ggccgagaag gtccaccgct ccctccaccg
     2821 tgtcaggctg gtagagctcg aaagcgctca tcatgtctct ggacatcgcc aaagctcctt
     2881 cagttagtgg ttacgcgcgc ctcaggcctg gctcacgcgg tcgccggtag ccgcgcgcat
     2941 caccgcattc aagtaatggt cgtaggcgcc gcaacggcac aagttgcccg acagcgcgtg
     3001 ccgcacctcg tcgcgcgtcg gcgtcggatt ctcgttgagc agcgcgacgg ccgacatgat
     3061 ctggcccggc gtgcagaaac cgcactgcgg gccgagctcg tcgatcatcg cttgttgaac
     3121 tttgtgcagc tcgccgttcg ggccctcgag gccttcgatc gtcagcacgg cgcgcccgcg
     3181 cacggcatgt gtcagcgtcg agcacgaata gctcgcgacg ccgtcgatca gcaccgtgca
     3241 ggcgccgcac tcgccgcggt cgcagccgag cttcgtgccc gtgaggccga gcttgtagcg
     3301 cagcgtcatc gcgagcgtct cgttcggcgc gacgtcgacg ggccgcatct ggccgttgac
     3361 gttcagcgtc agcaggcgct cgacgccggc ggccgaaccg ccgccgctcg agcagccggc
     3421 cagcgacagc agatagccgc cgcccgtgac ggccgcgccg gagaagatga cacctttaat
     3481 aaactggcgt ctcgacgtcg ctttcgagat gaacgagtcg aaccgcggct ccggatagct
     3541 ccggccgttg gtattgtcag ccatgaggaa gtctcccctc cgatgcgcct agacgtacgg
     3601 aaatcgagcc gatgctaggt gaaaagccgg ccccgctcaa caggccggaa ttggcgcttc
     3661 cgccgctcat gccgtgttga cctgcaaggg tttgtgcgac ggctcgcgct tggccgccgc
     3721 gttgacgatc atgtccggca tcacgggcgt ccggttgaaa tagtggccgc cgagcgcgtc
     3781 cgagatcgcg cataagagcg ccgcggccgc gcagccctga acgggctcgc cgataccctt
     3841 cgcgccgacc gggttttgcg cgtccgcgat gtcgaccgag gccgtccgca tcgtggccgg
     3901 cacgtcgagg taggtcaccg gcttcgcttg cagcagcccg acgttgccgg gcaggccgtt
     3961 ctgcgggtcg tagatgtgcc gctccgacgc ggcgagcccg aagcccatga cggcgccgct
     4021 cttgatctga tgatcgaggc cctgcgggtg cagcacggtg ccgcaatcgg cgacgccgac
     4081 gtagtcgaga atgtcgaact ttccggtttc cttatcgagc tcgatctcga tgaagcccgc
     4141 cgcgagcgcc ggcaccgtgc ccgtgagcgg gatgttgtcc ttcgcgacgc cgacgaggcc
     4201 ggtcccggcg acgccggcca ccgacgcctt cgtcatggcg ttcaagccgt cgggcgcctc
     4261 gtggccgtcg aacttgccgc cgagctcgat cgcgcgctgc gcggcctgcg cgtacgtgag
     4321 ccccttgctc gagtcgctct tcgcgaacac gcgctcgccg ccgatgtcgt agtcgtccgg
     4381 cgcgccgccg agatccatcg cggcgatctc cttgagcttc gcgacggcgt ccatcgccgc
     4441 ggcgtagttc gtgcgcgtca tcgtgaacga cgtgttgctg ccgaactggc ccaggttcca
     4501 cggcaagtgc ttgctgctgt cgccgcgcac gacttcgcag ttgtcccaat tcgccttcag
     4561 cacctcggcc gcgacgcgcg acgtcgccgt gtgcgaaaag gtgccgaggt tgccgacgcc
     4621 cgtgtggatg tggatcttgc cgtccggcgt gatccgcacg aggccgtcga agccgctgtt
     4681 gccggccggg tgaaaggccg tgccgacgcc gacggcgcgc accttggacc cgttaacttg
     4741 gccgctgccg gcctgccgct cggcccagcc gaacagctcg gcgcctcgat cgagcgcatt
     4801 cttcaagtag gcgctcgtca cgccgccctg catccggccg tacttcgcgg cgttgtcggg
     4861 ggcgttgatg cgtcggatcg cgacgcgatc gaccccgagc tgcttcgccg ccttgtcgat
     4921 cagcggctcg acggcggccg cgatctgatt ctgccccgga ccgcgctgcg ggccgcgcgg
     4981 cggcgtgttc gtcatcaccg agacgccgcg gaagcgcatc gcgggcggct ggtagacgag
     5041 cgagacggcg tcggcggccg acaaccagtc ggtgaacccc tgattggggc cgcactcctg
     5101 cactatataa aggtctacgg ccgtcacgcg gccgtcggcg gcaaagccca tcttgattcg
     5161 gccttggaac ccggcgcgcg ccgagcccat gaagtattcc tcgtggcgcg agacgcgcat
     5221 catgacgggg cggccgatct tcttcgccat cagcgccggg atcgccatca gcggataagc
     5281 tccgcccttc gagccgaagc cgccgccgca gaattccgag acgtagacga gattctcggg
     5341 ctcgacgccg atgtaccgtg caatcgtcgg gaacggaaag ctctggctct gcgtcgagcc
     5401 gtagaggtag cacttgccgt tctgccaaaa gctgagcgcg ctgcgcggct ccatcgagtg
     5461 atgcgcgagg ccggccgtca cgaacgtctc gtcgagcacg accttcgcgc gggcgaaacc
     5521 ggcgtcggtg tcgccgaacg accactccgc ggtcggtgcc ccctgcggca gttggccctc
     5581 ctgcacggcg gcgaaatcct gcgccgtcca cttgacgctg cgaagctccg ggccctgcac
     5641 cgagttgccg ttcgtgcgcg cgttcggccc gcccggatac aagctctgca gcggatcgac
     5701 cgtgaacggc agctcctcgt aggtgacttc gattttctcg agcgcctcgc tcgcgagctc
     5761 ttccgtgtcg gccgcgatgg cgaggatcgg atggccgacg aacagcggct catcggtcag
     5821 gatcgtctcc tgcgggtccg gcgggttcga gacctcggaa gcccgcaaga tgccatggac
     5881 gcctggcatc gcgagcgcgg ccttgtcgtc gacgttcgtg actcgcgcat gcggcatcgg
     5941 gctcgtcaag agcttcacga acaccatgcc ctcggcgcgg atgtcttccg agtacttggc
     6001 cttccccgtc accttcgcgt gcacgtcggg tggcgtgaaa ttcttgccga gcagatcaaa
     6061 ggccatgaga tccctccagt gaattgcgca cgagaacgcc cgcccgcaca tgatactccc
     6121 gagccgcggg ggtgtgcgaa acttgcgcct cgtcccttcc agcaaggcgg ccatggacgt
     6181 cggcacgtgg gagctcttga gctacgtcgt cacgacgatc ggcttgccgc tcgcgattct
     6241 cgtattcatt tacgagcaaa agaaagagcg cgacaacgag gacgaagagg tctaccagct
     6301 cctctccgac aactatcagg acttcttgcg catggcgctc ggccatcccg acctgcatct
     6361 gttcacgccc gagcaaacgc ccgagctcaa cgacgagcaa cgcgagcgca tgctgatcat
     6421 cttcaacatg ctgatctcgc tgttcgagcg cgcctatctg ctcttgtacg aggagaagat
     6481 gtcgtcgaag cacaagcgcc gctggctgtc ctgggaggac tacatgcgcg agtggtgccg
     6541 ccgcgacgat ttccgcgcgc tgctgccggt gctgctcgag ggcgaagacc ccgagttcgg
     6601 cgcgtacatc cgtgcgctcg ccgaggacgt ctctccgacc ggctcgttgc ttctgagctc
     6661 tagctgatcg tcagttatcg cgcgctcgtc gcggcgcgct cgcgcgcgcg gcccgcggag
     6721 agcgcgttcg gcagcgcgag attgccttcg tggcaggcgt actcgaacat cacgtagcgc
     6781 ggatcgcgat tcaggggcag cttgacggtc cacggcgccg tgtacacctt cggatccgtg
     6841 accgtcacct cgtactcgat gacgtcctcg ctgacgcgc
//
LOCUS       DASSNN010000324         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_800853, whole genome shotgun sequence.
ACCESSION   DASSNN010000324 DASSNN010000000
VERSION     DASSNN010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800853"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFL84_05905"
     CDS             <1..326
                     /locus_tag="VFL84_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608191.1"
                     /translation="RVSLPPIGAGGRPPNAARLAPLPQDRDDGTEEIIVIGQGWRLPD
                     LGSEWRARQKETENVGRFSATALPLYDPAEPPLHSDAFTAGGEERRHGYIELFRLRFG
                     RRSTD"
     gene            complement(332..1189)
                     /locus_tag="VFL84_05910"
     CDS             complement(332..1189)
                     /locus_tag="VFL84_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET7608192.1"
                     /translation="MPAAGLRHHAAACVALAAALAPAAAAHADPANVVNALRAQGCGG
                     VAAAGAPVRRDSALDAAARELARNTKLADALARVGYPAASSSSFNIRGARDDASIRNM
                     LETRYCERINDPRLVELGFHQSGDRTWIVMAGRTEKPFAALQDPSGVAQRVVELVNAA
                     RAAPRRCGNDSFEPAPPLKAATALTAAASLHSLDMAARGELSHDGSDGSESGERMTRA
                     GYSWRIAGENVASGQHDAEAVVAAWLSSPGHCATLMDARYSETGVAFALAPGKNPAVY
                     WTQDFAAPR"
     gene            complement(1200..2081)
                     /locus_tag="VFL84_05915"
     CDS             complement(1200..2081)
                     /locus_tag="VFL84_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608193.1"
                     /translation="MRNICVCLAFMGLAAQPLSAQSAAGCDRACLAGVMTAYLDSLVA
                     HDPNKAPLAANARFTEDAKELRVGEGLWKTAGKLRPFRTDFLDAATGTAATHAVVEEN
                     GMPVLLAARLKVDGRRIAEVETIVVRSREEGALFAPEALAQPSAAMVTAPPAAARMPR
                     DKLVEIALRYPGGLKVGSFEKSDVPFAPNAYRLENGVRMAGPGCTFRPPSCENMRGQQ
                     IPTLAGIVAHVVAVDEENGTVLVWMDFGPGSLPGPPNAPSRSLVTFEAFKIYGGQIHA
                     VEAVFEGMPPNTPSGWR"
BASE COUNT          317 a          792 c          786 g          314 t
ORIGIN      
        1 tgcgcgtatc gctgccgccg atcggcgccg ggggacggcc gccgaacgcc gcgcggctcg
       61 ctccgctgcc gcaagaccgc gacgacggca ccgaggaaat catcgtcatc ggccaaggtt
      121 ggcgcttgcc cgacttgggc agcgagtggc gcgcgcggca gaaggaaacc gagaacgtgg
      181 gacggttctc cgcgacggca ctgccgctct acgaccccgc cgagccgccg ctgcacagcg
      241 acgcgttcac ggccggcggc gaggagcgca ggcacggcta catcgagctg ttccgtctgc
      301 gcttcgggcg ccgttccacc gactgacgcc ctcaccgcgg cgcggcgaaa tcctgcgtcc
      361 agtaaaccgc ggggttcttg cccggcgcca gcgcgaacgc gacgccggtc tcggagtagc
      421 gcgcgtccat gagcgtcgcg caatggccgg ggctcgagag ccaggcggcg acgacggcct
      481 ccgcgtcgtg ctgccccgac gccacgttct cgcccgcgat gcgccacgaa tagcccgcgc
      541 gcgtcatgcg ctcgcccgac tcgctgccgt ccgagccgtc atggctgagc tcgccgcgcg
      601 cggccatgtc gagcgagtgc agcgacgcgg cggccgtcaa cgccgtcgcc gccttgagcg
      661 gcggagcggg ctcgaagctg tcgttgccgc agcggcgcgg tgccgctcgc gcggcgttga
      721 cgagctcgac gacgcgctgc gcgacgcccg agggatcctg caatgcggcg aacggcttct
      781 cggtgcggcc ggccatgacg atccaagtgc ggtcgccgct ctggtggaag ccgagctcga
      841 cgagcctcgg atcgttgatg cgctcgcaat agcgcgtctc gagcatgttc cgaatcgacg
      901 cgtcgtcgcg cgcgccgcgg atgttgaacg acgacgagct cgcggccgga tagcccacgc
      961 gagccagcgc gtcggcgagc ttcgtattgc gggcgagctc gcgcgcggcc gcgtcgagcg
     1021 cgctatcgcg ccgcaccggc gcgcccgcgg cagccacgcc tccgcatcct tgcgcgcgca
     1081 gcgcgttgac gacgttggcc gggtcggcgt gcgcggcggc cgccggcgcc agcgccgcgg
     1141 cgagcgcgac gcacgctgcc gcatgatgac gcaaaccggc cgcgggcacg tctcgtcctt
     1201 cagcgccagc ccgacggcgt gttcggcggc attccctcga atacggcttc gacggcatgg
     1261 atttgcccgc cgtagatctt gaacgcctcg aacgtcacga gcgagcgcga cggcgcattg
     1321 ggagggcccg gcaacgagcc ggggccgaaa tccatccaca cgagcaccgt gccgttctcc
     1381 tcgtcgacgg cgacgacgtg cgcgacgatt ccggccagcg tcggaatctg ctggccgcgc
     1441 atgttctcgc agctcggcgg ccggaacgtg cagccggggc ccgccatgcg cacgccgttc
     1501 tcgagccggt acgcgttcgg cgcgaacggc acgtcggact tctcgaagct gccgaccttg
     1561 agaccgccgg gataacgcag cgcgatctcg acgagcttgt ctcgcggcat gcgcgcggcg
     1621 gccggcggcg ccgtgaccat cgcggccgat ggttgcgcga gcgcctcggg cgcgaacagc
     1681 gcgccttcct ctctgctgcg cacgacgatc gtctcgactt ccgcgatgcg gcggccgtcg
     1741 actttgaggc gtgccgcgag cagcacgggc atgccgttct cctcgacgac ggcgtgcgtc
     1801 gcggccgtgc cggtggccgc gtcgaggaaa tcggtacgga acggccgcag cttgcctgcg
     1861 gtcttccaca gcccttcgcc gacgcgaagc tctttcgcgt cttcggtgaa acgcgcgttt
     1921 gcagccagcg gcgccttgtt cggatcgtgc gcgacgagcg agtcgagata ggccgtcatg
     1981 actccggcaa ggcaggcgcg gtcgcagccg gcggcgcttt gcgcggagag cggctgcgca
     2041 gcgagaccca tgaacgcgag gcaaacgcaa atgttacgca cggtgagctc ccttcgaggg
     2101 acgcgtgagt attgcactaa cacgagccgc cagggcaact cgacggggcc ttagccatcg
     2161 tcgcgcctcg tcgcacgcgg cgagccgcgc cgccgccacg gcgtctgcg
//
LOCUS       DASSNN010000325         3230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_294550, whole genome shotgun sequence.
ACCESSION   DASSNN010000325 DASSNN010000000
VERSION     DASSNN010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294550"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFL84_05920"
     CDS             complement(<1..338)
                     /locus_tag="VFL84_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7608194.1"
                     /translation="MNIDSTLPLPGKDAQAHSARVVAHVRDEIAKCGGFIPFSRYMEL
                     VLYAPGLGYYVAGARKFGADGDFVTGPELTPLYGAALARQVEAILAAARGRTVVELGA
                     GSGALAASLL"
     gene            429..1166
                     /locus_tag="VFL84_05925"
     CDS             429..1166
                     /locus_tag="VFL84_05925"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HET7608195.1"
                     /translation="MEGKVVLITGGAKRVGAAICRRLHKAGASIMLHFRSSAGEARLL
                     QAELNHLRANSVALIQADLLDVAKLPSLAEQTVQSFGRLDALVNNASTFYATMIGEIE
                     VEQWNDLIGTNLQAPLFLSQAAAPALRKTQGAIVNITDIHADRPLKSYVVYSVAKAGL
                     VGLTRSLARELAPDVRVNAIAPGPILWPDEEVFDELARQRIISHTPLKREGTPDDIAK
                     AVHFLLADAPYVTGETISVDGGRHMAL"
     gene            complement(1223..1942)
                     /locus_tag="VFL84_05930"
     CDS             complement(1223..1942)
                     /locus_tag="VFL84_05930"
                     /inference="COORDINATES: protein motif:HMM:NF016627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HET7608196.1"
                     /translation="MTLSRRDLEQGRMREVYVQALAEGHALTDEQLSASLTATLSAKP
                     KGAGWWVFAYGSLLWNPLFPFAESRRASVHGLHRRFCLWSLASRGRPTAPGLVLALDR
                     GGSCRGVAYRLPAPCAIDELHLLWRREMVTGAYAPRWLHVDCDGRRQVALGFVVRRDH
                     AQYTRKLTIDEQAQVIAHACGVFGSSADYLERTRVSLATHGIVDPYLERLAARVAARG
                     CVAPMAIQEPHATAATGEQPV"
     gene            complement(1977..2144)
                     /locus_tag="VFL84_05935"
     CDS             complement(1977..2144)
                     /locus_tag="VFL84_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608197.1"
                     /translation="MKRAKQHAVPKPRNPVARSPLLRKGGAHGKSVGALRRKANVELQ
                     KLRRDPNAADE"
     gene            complement(2141..>3230)
                     /gene="hemW"
                     /locus_tag="VFL84_05940"
     CDS             complement(2141..>3230)
                     /gene="hemW"
                     /locus_tag="VFL84_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HET7608198.1"
                     /translation="PYCDFNSHAAPDALPEDAYVEALITDLESALPSIWGRKVSTVFI
                     GGGTPSLFAAAAIDRLLGAVRARVQLSPDAEITLEANPGTFEREKFAGFYAAGVNRLS
                     LGIQSFDDRCLRALGRIHGAEEARRAAEAALMIFGNVNFDVMFALPEQTPADAADDVA
                     AALAFAPPHLSFYHLTLEPNTLFHRHPPPLPDDETAADIEDAMCAALDRAGYVHYETS
                     AHAKPGYECRHNVNYWRFGDYLGIGAGAHSKLSFADRIVRQLRFKQPKQYLERIAIGD
                     ALQENIEIARRDLPFEFMLNALRLTDGVPAALFAERTGMPLSIAARPIAEATRRGLLD
                     PDPTVLRPTPLGRRFLNDLQSIFLASPR"
BASE COUNT          528 a         1135 c         1078 g          489 t
ORIGIN      
        1 tcgagcagcg atgccgcgag cgcgccgctg cccgccccca gctcgacgac cgtacgccca
       61 cgcgcggcgg ccaatatcgc ctcgacctgc cgtgcgagcg cggcgccgta gagcggcgtg
      121 agctccggac cggtgacgaa atcgccgtcc gcgccgaact tgcgcgcgcc tgccacgtaa
      181 tagccgagcc caggcgcgta cagcacgagc tccatgtagc ggctgaacgg gatgaagccg
      241 ccgcacttcg cgatctcgtc gcgcacgtgc gcgacgacgc gcgcgctgtg cgcctgcgca
      301 tccttcccgg gcagcggcag cgtcgaatcg atgttcattg ccggatgccg cctgccgcgt
      361 tcgcacgcgt tcggcgactg tgctagattg gccgcagtct aatcgacgtg cggataccag
      421 ggcgcggctt ggaaggcaag gtcgtcttga tcaccggtgg cgccaagcgc gtgggcgccg
      481 cgatctgccg gcggctgcac aaggccggcg cgtcgatcat gcttcacttc cgctcttccg
      541 ccggcgaagc gcggcttctg caggcggagc tcaatcacct gcgcgcgaac tcggtcgcgc
      601 tgatccaggc cgatttgctc gatgtcgcca agctgccctc gctggccgag cagacggtgc
      661 agtcgttcgg ccggctcgac gcgctcgtca acaacgcatc gacgttctat gcgacgatga
      721 tcggcgagat cgaagtcgag cagtggaacg acctgatcgg cacgaacctg caggcgccgc
      781 tgttcctttc gcaggcggct gcgcccgcgc tgcgcaagac gcagggggcg atcgtcaaca
      841 tcaccgacat ccacgccgat cggccgctca agagctacgt cgtctattcg gttgcgaaag
      901 cggggctcgt cggtcttacg cgctcgctcg cgcgcgagct cgcgcccgac gtgcgggtca
      961 atgcaatcgc accgggtccg atcctgtggc cggacgagga agtcttcgac gagctcgcgc
     1021 ggcagcggat catctcgcac acgccgctca agcgcgaagg cacgcccgac gatatcgcca
     1081 aggccgtgca cttcctgctt gccgacgcgc cttacgtcac cggcgagacg atcagcgtcg
     1141 acggcggacg ccacatggcg ctgtagcagc tcgactcgcg cggcgcacct gcgcgattga
     1201 cgacgcgtaa ttccccggcc cgctaaaccg gctgctcgcc ggtcgcggcc gtcgcatgcg
     1261 gctcctgaat ggccatcggc gcgacgcatc cacgcgccgc cacgcgagcg gcgaggcgct
     1321 cgagatacgg atcgacgatg ccgtgcgtcg cgagcgacac gcgcgtgcgc tcgagatagt
     1381 ccgcggacga gccgaacacg ccgcacgcgt gcgcgatcac ctgcgcctgc tcgtcgatcg
     1441 tcagcttgcg cgtgtactgc gcgtggtctc gcctgacgac gaagccgagc gcgacctgcc
     1501 ggcggccgtc gcaatcgacg tgcagccagc ggggtgcgta tgcgcccgtc accatctccc
     1561 gccgccagag caggtgcaat tcatcgatcg cacagggcgc gggcaggcgg tacgcgacgc
     1621 cgcgacacga gccgccgcgg tcgagcgcaa gcacgagccc gggcgcagtc ggccgccccc
     1681 gcgacgcgag cgaccagagg cagaagcgcc ggtgcaatcc gtgcacggac gcgcggcgcg
     1741 attccgcgaa cggaaaaagc ggattccaca acagcgaccc gtatgcgaac acccaccagc
     1801 cggcaccttt gggcttcgcc gaaagcgtgg cggtcagcga ggccgacaac tgctcgtcgg
     1861 tgagcgcgtg gccctcggcc agcgcctgca cgtacacctc ccgcatcctt ccctgctcga
     1921 gatcgcgccg cgacagcgtc atggcgaatc cgcgatttcg acgtggcggc cgcgcatcac
     1981 tcgtccgccg cgttcggatc acgccgcagc ttctgcaatt cgacgttcgc ctttcgccgc
     2041 aaggcgccga ccgacttgcc gtgcgcgccg cccttgcgca acaacggcga gcgcgcgacc
     2101 ggattgcgtg gcttgggcac ggcgtgctgc ttcgcgcgct tcatcgcggc gacgccagga
     2161 agatcgactg caggtcgttc aggaatcgcc gtccgagcgg cgtgggtcgc aagacagtcg
     2221 gatccggatc gagcaacccc cggcgcgtcg cttcggcgat cggccgcgcc gcaatggaca
     2281 acggcatccc ggtgcgctcg gcaaaaagcg ccgcgggaac gccatcggtc agccgcagcg
     2341 cgttcagcat gaattcgaac ggcaggtcgc gccgcgcaat ctcgatgttc tcctgcagcg
     2401 cgtcgccaat cgcgatgcgc tcgagatact gcttcggctg cttgaagcgc agctgccgca
     2461 cgatccgatc ggcaaaggag agcttggagt gcgcgccggc cccgatgccc agatagtcgc
     2521 cgaagcgcca gtagttgacg ttgtgccggc actcgtaccc cggcttcgcg tgcgccgatg
     2581 tctcgtaatg cacgtagcct gcacggtcga gcgccgcgca catcgcgtcc tcgatgtcgg
     2641 cggccgtctc gtcgtcgggc agcggcggcg gatggcgatg gaacaacgtg ttcggctcga
     2701 gcgtcaggtg atagaacgac aggtgcggcg gcgcgaacgc cagcgcggcc gcgacgtcgt
     2761 ctgcggcatc ggcaggcgtc tgctccggca atgcgaacat cacgtcgaag ttgacgttgc
     2821 cgaagatcat cagcgccgct tccgccgcgc gccgcgcttc ctcggcgccg tgaatgcgtc
     2881 cgagcgcgcg caagcagcgg tcgtcgaagc tctggatgcc gagcgagagc cgattgacgc
     2941 cggcggcgta gaagcccgcg aacttctccc gctcgaacgt tcccggattc gcctccagcg
     3001 tgatttcagc gtcgggcgaa agctgcacgc gcgcgcgcac cgccccgagc agccggtcga
     3061 tcgccgcggc cgcaaacagg ctcggcgtgc cgccgccgat gaacaccgtg gataccttgc
     3121 ggccccagat cgacggcagc gcgctttcga gatcggtgat cagtgcttcg acgtacgcgt
     3181 cttccggcag cgcgtccggc gcggcgtgcg aattgaaatc gcagtacggg
//
LOCUS       DASSNN010000326         4857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_801219, whole genome shotgun sequence.
ACCESSION   DASSNN010000326 DASSNN010000000
VERSION     DASSNN010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801219"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..672)
                     /locus_tag="VFL84_05945"
     CDS             complement(28..672)
                     /locus_tag="VFL84_05945"
                     /inference="COORDINATES: protein motif:HMM:NF040011.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6151 family protein"
                     /protein_id="HET7608199.1"
                     /translation="MADTFDLPLRCRCGAVRGVARDAVPSKINHCFCYCDDCQAFVHH
                     LGRADDILDAHGGTEVVQMSPANVRFTAGTDKIAAIRLTEKGLVRWYASCCNTPIGNT
                     MAASGVPFIGVVGAFIAAPSAALGPIRGRGFAKSAKGGRDAVPKDGLRDLVMVARVLV
                     KLLRWRLRGDQTRSPLFDSATGKPLVEPRVLGAAEREDLRRRCARWRGEAVGGG"
     gene            complement(678..2345)
                     /locus_tag="VFL84_05950"
     CDS             complement(678..2345)
                     /locus_tag="VFL84_05950"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET7608200.1"
                     /translation="MSCRARFATALLAASFVPAALAQPPDLVLLDGKVVTVDADSSLR
                     EALAIRDGKILRVGSTAEMRGLAGPDTRVVELRGRTVIPGLIDSHLHAVRAALSFSTE
                     VNWIGARSLREALGRLSEAAARRPSGSWLIVAGGWNELQFAERRRPTQAELEAAAPSN
                     PVYVQLGYGWVVMTDDGFAKLGIRSEADLPAGGTLVRDGERLTGAISGGQGAIIALFD
                     RLPKPTFAEQVQGTREFFRELNRLGLTGVVDPGGNNLFPSDYQALFDVWRRGELTVRV
                     AYSLNGQTAGEELKELQNLTALLPMGFGDDRLHFNGLGERITFAMNNNPEPTAEHKER
                     YYEIVRWAAERGMTITMHWGPDETVPHLLEIFERVNREVPIAPLRWSIAHLNDASDET
                     LKRMRDLGVGWTVQDAMYFGGEDLVRRQGADAARRIPPVVTGDRLGLAIGAGTDAHRV
                     ATYNPFTSLKWFLDGKTVAGTPIRGPEETPDRLTALKFYTLGSAWFSFDEDVRGSLEV
                     GKLADLAVLSADYLTVPLEEIGNIESVLTLLGGEVVYAAGDFAALED"
     gene            complement(2342..2959)
                     /locus_tag="VFL84_05955"
     CDS             complement(2342..2959)
                     /locus_tag="VFL84_05955"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET7608201.1"
                     /translation="MISVEPIELEGHGVRLEPLAQKHERELAAAAAEGALWELWYTVV
                     PAPAETKSYIETALAGQKEGRMLPWAVRNLATGAVVGSTRFHDVAAHIDRVEIGYTWY
                     AKSWQRSYVNTSCKLLLLTHAFERIGCKVVGLRTDKFNFASQRAIEALGAKKDGVIRH
                     FGTRRDGSARDVVMYSIVLSEWPEIKLHLTRRLARHGMRASGVDT"
     gene            3170..4828
                     /locus_tag="VFL84_05960"
     CDS             3170..4828
                     /locus_tag="VFL84_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HET7608202.1"
                     /translation="MKTANVLSICASSGVAILALAAAAAKAEDVEATLRVDAVKVYPR
                     TAAVTRRGQLSIPAGEHRLIVRGLPDPVDSGSLRISAGSRELRLGGVELQKIVATDLV
                     SGAERALSARLTALEDQRAAVQDEIPTAETQLKLIDALAAVPGKGDEDLFSRSANLPA
                     TLAAIGGGAAEARSSIRAAKISLRALDAQIAATKAELDKVRTAKKSTTEVRATVFASA
                     PVTTEISVEYRVNDAGWTWLYEARLDTLDESLSFARQASITQGSGEDWANAQVTVTTA
                     RPATDAGSPRVASLFVELQDYFVSGGGGGGGELEEIVVTGSRIRGRRNGAPQTEEAPH
                     VTAESFATEFVAEYRIPGRVSIGADRQARVYPVSEEMFELELLARAVLPAGPDARLEA
                     AFHYDGDLPIDAGRVQLYRDGAYIGAAALPLLLPGSDVRVPFGIDERIRIVVRDERTE
                     SGSRGLANRQRLEEQRRRYEVTSYHAAALPIEIIDRVPVPTESGIEVKVLDGATPPTL
                     RDLDGKEGVYLWRFDGTPRHTETIHHYYSVRYPSDRMLAATEGN"
BASE COUNT          767 a         1705 c         1644 g          741 t
ORIGIN      
        1 cgatcacgct ggcggctcag ctattgatca gccgccgcca acggcctcgc cccgccaccg
       61 agcgcagcgc cgcctgagat cctcgcgctc ggccgcgccg agcacgcgcg gctcgacgag
      121 cggcttgccc gtcgcggaat cgaacaatgg cgagcgcgtc tggtcgccgc gcaaacgcca
      181 gcgcagaagc ttcacgagca cgcgcgccac catgacgaga tcgcgcaggc cgtccttcgg
      241 cacggcgtcg cggccgccct tcgcgctctt cgcgaatccg cggcctcgaa tcggcccgag
      301 cgcggccgac ggcgccgcga tgaacgcgcc cacgacgccg atgaacggca cgccgctcgc
      361 ggccatcgtg ttgccgatcg gcgtattgca gcagctcgcg taccagcgca cgagcccttt
      421 ctcggtgagg cggatcgccg cgatcttgtc cgtgcccgcg gtgaagcgca cgttggcggg
      481 cgacatctgc acgacctccg tgccgccgtg cgcgtcgaga atgtcgtcgg ctcggccgag
      541 gtgatgcacg aacgcctggc aatcgtcgca gtagcagaag cagtggttga tcttgctcgg
      601 cacggcgtcg cgcgcaacgc cgcgcacggc gccacaccgg caacgcagcg gcaggtcgaa
      661 cgtatccgcc atcgccttca gtcctcgagc gccgcgaagt cgcccgccgc gtatacgacc
      721 tcgccgccga gcagcgtcag caccgattcg atgttgccga tctcctcgag cggcaccgtg
      781 agatagtcgg ccgaaagcac ggcgagatcc gcgagcttgc ctacctcgag cgagccgcgc
      841 acgtcctcgt cgaacgagaa ccacgcgctg cccagggtgt agaacttgag cgccgtgagt
      901 cggtccggcg tctcctcggg cccgcgaatc ggcgtgcctg ccacggtctt gccgtcgaga
      961 aaccacttca gcgacgtgaa cgggttgtac gtcgcgacgc gatgcgcgtc ggtgccggcg
     1021 ccgatcgcta atcctaagcg atcgcccgtc acgacgggcg gaatgcggcg cgccgcgtcg
     1081 gcaccctgcc ggcgcacgag gtcctcgccg ccgaagtaca tcgcgtcttg cacggtccag
     1141 ccgacgccga ggtcgcgcat gcgcttcaac gtctcgtcgg acgcgtcgtt caagtgcgcg
     1201 atcgaccagc gcagcggcgc gatcggcact tcgcggttca cgcgctcgaa gatctcgagc
     1261 aagtgcggca cggtctcgtc ggggccccag tgcatcgtga tcgtcatgcc gcgctcggcg
     1321 gcccagcgca cgatctcgta gtagcgttcc ttgtgctcgg ccgtcggctc ggggttgttg
     1381 ttcatcgcga acgtgatgcg ctcgccgaga ccgttgaagt gcaggcggtc gtcgccgaag
     1441 cccatcggca acagcgcggt caggttttgc agctccttta gctcctcgcc ggccgtctgg
     1501 ccgttcaagc tgtacgcgac gcggaccgtg agctcgccgc ggcgccagac gtcgaacagc
     1561 gcctggtagt cggacggaaa cagattgttg ccgccgggat cgacgacacc cgtgagcccg
     1621 agccgattca gctcgcgaaa gaactcgcgc gtgccctgca cttgctcggc gaacgtcggc
     1681 tttggcagcc gatcgaacag cgcgatgatc gcgccttgcc cgccgctgat cgcgcccgtc
     1741 agccgctcgc cgtcgcggac gagcgtgccg ccggcgggca ggtccgcttc gctgcggatc
     1801 ccaagcttcg cgaagccgtc gtcggtcatg acgacccagc cgtagccgag ctgcacgtac
     1861 acggggttcg acggcgccgc ggcttcgagc tcggcctgcg tcggccggcg ccgttccgcg
     1921 aactgcagct cgttccagcc gccggcgacg atgagccagc tgcccgacgg cctgcgagcg
     1981 gccgcctcgc tcaagcggcc gagcgcctcg cgcagcgagc gtgcgccgat ccaattgacc
     2041 tcggtgctga agctcaaggc ggcccgcacc gcgtgcaggt gcgagtcgat gagccccggg
     2101 atcaccgtgc ggccgcgcaa ctcgacgacg cgcgtatcgg gccccgcgag gccgcgcatc
     2161 tcggccgtgc tgcctacgcg caaaatcttg ccgtcgcgaa tcgcaagcgc ctcgcgcagc
     2221 gaagagtcgg cgtcgacggt gacgactttg ccgtcgagca gcacgagatc cggcggctgc
     2281 gccaaagcgg cgggtacgaa cgacgcggcg agcaaagccg tggcgaaacg agctcggcag
     2341 ctcatgtatc tacacccgag gctcgcatgc cgtgccgcgc gagccgcctc gtaagatgca
     2401 gcttgatctc gggccattca ctcaaaacga tgctgtacat gacgacgtcg cgcgcggagc
     2461 cgtcgcgccg cgtgccgaag tggcggatca cgccgtcttt cttcgcgccg agcgcctcga
     2521 tcgcgcgctg cgaagcgaag ttgaacttgt cggtgcgaag gccgacgacc ttgcagccga
     2581 tgcgctcgaa cgcatgcgtg agcagcagta gcttgcagct cgtattgacg tagctgcgtt
     2641 gccagctctt cgcgtaccag gtgtagccga tctcgacgcg gtcgatgtgg gcggcgacgt
     2701 cgtgaaagcg cgtgctgccg acgacggcgc ccgtcgcgag attgcgcacg gcccacggca
     2761 gcatgcggcc ttccttctgg ccggcgagcg cggtttcgat gtagctcttc gtctccgcag
     2821 gcgccggcac gaccgtgtac cagagctccc acagcgcgcc ctccgcggcc gccgccgcga
     2881 gctcgcgctc gtgcttttgc gcaagcggct cgagccgcac gccgtggcct tcgagctcga
     2941 tgggctcaac cgagatcatc gcttgccaac cccccgtcgt ctcgtcgttc ttgtgcctac
     3001 gccggccgtc gtccggtgcg cgaagaagcg tacgcgccag tttggttttg cgccccccaa
     3061 agtgcaaatg ccgtgccgcc gccgtcgcga cgatctgaac cttttcgact cgccgcgcgc
     3121 ttaacccact gacagcgccg cgctgtctcg agatgacgag gtgacgcaca tgaagacagc
     3181 caacgttctt tcgatctgcg cgtcgtcggg cgtcgcgatt ctcgcgctcg ccgccgctgc
     3241 ggccaaagcc gaggacgtcg aggcgacgct gcgcgtcgac gcggtcaagg tgtatccgcg
     3301 gacggccgcg gtcacgcgcc gcggccaact gagcattccc gccggcgagc accgcttgat
     3361 cgtgcgcggc ctgcccgacc cggtggattc gggcagcctg cgcatttccg cgggctcgcg
     3421 cgagctgcgg ctcggcggcg tcgagctcca gaagatcgtc gcgacggact tggtcagcgg
     3481 cgccgagcgc gcgctgagcg cgcggctcac cgctctcgag gatcagcgcg cggccgtgca
     3541 ggacgagatc ccgacagccg agacgcagct gaagctcatc gatgcgctcg ctgccgtgcc
     3601 gggaaaaggc gacgaagatt tattctcgcg cagcgccaat ctgcccgcga cgctggcggc
     3661 gatcggcggt ggcgccgcgg aggcgcgctc gagtatccgc gccgccaaga tcagtctgcg
     3721 cgcgctcgac gcgcaaatcg cggcgaccaa agccgagctc gacaaggtgc gcacggcgaa
     3781 gaagagcacc acggaggtcc gcgcgacggt gttcgcgagc gcgccggtca ccacggagat
     3841 ctccgtcgaa taccgcgtca acgacgcggg ctggacctgg ctatacgagg cgcggctcga
     3901 cacgctggac gagtcgctct cgttcgcgcg gcaagcatcg atcacgcaag gctccggcga
     3961 ggactgggcg aacgcgcagg tcacggtcac gacggcgcgg cccgcaaccg atgccggctc
     4021 gccgcgtgtc gcgtcgctgt tcgtggagct gcaggactac ttcgtgagcg gcggtggcgg
     4081 cggcggcggc gagctcgaag agatcgtcgt gacgggcagc cgtatccgcg ggcgacggaa
     4141 cggcgcgccg caaacggagg aagcgccgca cgtcacggcc gagtcgttcg cgaccgagtt
     4201 cgtcgccgag taccggattc ccggaagagt cagcatcggc gccgaccgtc aggcgcgcgt
     4261 gtacccggtg tcggaggaaa tgttcgagct ggagctgctg gcgcgcgccg tattgcccgc
     4321 cggtcccgat gctcgtctcg aggcggcgtt ccactacgac ggcgatttgc cgatcgacgc
     4381 cggacgagtc cagctctatc gcgacggcgc gtacatcggc gcagcggcgt tgcccctgct
     4441 gctgccgggc tccgacgtgc gcgtgccctt cggcatcgac gagcgcattc gcatcgtcgt
     4501 gcgcgacgag cgcacggagt ccggctcgcg cggcctcgcg aatcgccaga gactcgaaga
     4561 gcaacgccgc cgctacgaag tcacgagcta ccacgcggcc gcgctgccga tcgagatcat
     4621 cgatcgcgtg ccggtgccga cggaatccgg gatcgaggtc aaggtgctcg acggcgcgac
     4681 gcctccgacg ctgcgcgatc tcgacggcaa ggagggcgtc tacctgtggc gcttcgacgg
     4741 cacgcctcgg cacacggaga cgatccatca ctactactcg gtgcgctacc cgagcgatcg
     4801 catgctcgcg gccacggaag gcaactagtc acgcgccggc gcagcggcgt gacgcgc
//
LOCUS       DASSNN010000327         3562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_437413, whole genome shotgun sequence.
ACCESSION   DASSNN010000327 DASSNN010000000
VERSION     DASSNN010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437413"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFL84_05965"
     CDS             complement(<1..491)
                     /locus_tag="VFL84_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET7608203.1"
                     /translation="MRRVAVTGMGLVAPCGNSPEEFFANLVAGRSGIGVLPKMSARLA
                     TRIAAAASFDASAHFPAPRLRMLDRVSQLALVAAAQALRDAAIELSDDERAAAGVFLG
                     TGMGGAATTDDGYNTLYRESSDRIKPYTVLMAMNNAAASWIGIEQRLSGPNLTFSTAC
                     SSSA"
     gene            complement(498..761)
                     /locus_tag="VFL84_05970"
     CDS             complement(498..761)
                     /locus_tag="VFL84_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET7608204.1"
                     /translation="MTTTFETMKRIIAKDYELAPERLMPDTPLADIELDSLAITELIF
                     ALEDEFNVTAKTNGQGMQTLGDIAAYIDQLIAERDAPAARTGS"
     gene            complement(758..2050)
                     /locus_tag="VFL84_05975"
     CDS             complement(758..2050)
                     /locus_tag="VFL84_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7608205.1"
                     /translation="MTGRYDAIVIGAGVAGGTAAILLAQAGWSVALVEKRAFPRRKVC
                     GECIAAPNLALLDALEVGAEFAQLAGPELERVGLYVGDDTICADLPRLEGANAGFARA
                     LGRDRLDALLLSRAARLGVAIRQPCAVKEIERHGARYACRLAVEQRDAGRIEAPVLIG
                     AHGSWEPQPWQECAARRARRSDLVAFKANFSGASLPPGLLPVLAFRGGYGGMVVADGG
                     LLTLACCVRRDALREWRAAAPRSTAGDAVQAALAARCRGVRLALERARREGPWLSVGP
                     VRPAMRAAWSERTGFAVGNAAGEAHPILGEGISMAMQSAWLLCRALARERDVLSGGGP
                     QTAVAREYAREWRRHFAARVRFAAVLAQLAMRPQHARPLLPLLRAAPGLLTAAARIGC
                     KVKPLDELTSGPASNGRQRERARTMVAATVPRLGDKQA"
     gene            complement(2047..2784)
                     /locus_tag="VFL84_05980"
     CDS             complement(2047..2784)
                     /locus_tag="VFL84_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7608206.1"
                     /translation="MIAAARAASAQAGWPRRVVAELLDELPPDDPRALRSRRDLRLVN
                     RIMGHSALLARALDDVMTGPAPRLVELGAGDGTLLERLARRRARRWPHAAVTLLDAQP
                     TVTHATLAALRDLGWTADVVAADALEWLERSRPDRDAVVVANLFVHHFEGERLARLLA
                     GVAAQATAFVCCEPRRARLALAGSHLLWAIGCNDVTRHDAVVSVHAGFRDRELTAEWR
                     RAAGAGWTAYEKPAGAFSHLFVARRAP"
     gene            complement(2781..>3562)
                     /locus_tag="VFL84_05985"
     CDS             complement(2781..>3562)
                     /locus_tag="VFL84_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET7608207.1"
                     /translation="DANLERRDIDAIVVSTCTGYLCPGLSGYVIERLGLRPDLAAFDL
                     VGHGCAAALPNWQIADALLAGRRSENVLSVCVEVSSAAMYLDNDPGVLVSACLFGDGA
                     GAAVLSRRSAADKRSIECKSIGSLLNPVEREALRFEQRSGMLRNVLTRPVPKLAASHA
                     ERVLDDVLGRAGVDRAEIAAWVWHAGGRDVLLALRSRLGLTEEQTRYSAGILREYGNL
                     SSAFVYFVLEAALADRARGGWWWMSSFGAGFSCHGALLEVA"
BASE COUNT          514 a         1309 c         1269 g          470 t
ORIGIN      
        1 gccgacgacg agcacgccgt cgagaacgtc aggttcgggc ccgacagccg ctgctcgatc
       61 ccgatccaag acgccgcggc gttgttcatc gccatcagca cggtgtacgg cttgatgcga
      121 tccgagctct cgcggtacag cgtgttgtag ccgtcgtcgg tcgtcgccgc gcctcccatg
      181 ccggtgccca agaagacgcc cgcggccgcg cgctcgtcgt cgctgagctc gatcgcggcg
      241 tcgcgcagag cctgcgccgc cgccacgagc gcgagctggc tcactcggtc gagcatgcgg
      301 agccgcggcg ccgggaaatg cgcgctcgcg tcgaacgacg ccgccgccgc gatgcgtgtc
      361 gcgagccgcg cgctcatctt gggtaggacg ccgatgcccg agcgtcccgc gacgaggttc
      421 gcgaagaatt cctcagggct gttgccgcat ggcgcgacga gccccatccc cgtgacggcg
      481 acgcgcctca tcgggcgtta cgatcccgtg cgtgccgcgg gcgcgtcgcg ctccgcgatc
      541 aactgatcga tgtacgccgc gatgtcgccg agcgtttgca ttccctgtcc gttggtcttc
      601 gcggtgacgt tgaactcgtc ctcgagcgcg aatatgagct ccgtgatcgc gagcgagtcg
      661 agctcgatgt ccgcgagcgg cgtatcgggc atcaggcgct cgggcgcgag ctcgtaatcc
      721 ttggcgatga tgcgtttcat cgtttcgaaa gtcgtcgtca tgcctgtttg tctccgagcc
      781 gcggcaccgt cgcggcgacc atagtacgcg cgcgctcacg ctgacgaccg ttgctcgcag
      841 gcccggacgt tagctcgtcg agcggtttga ctttgcagcc tatccgtgcc gccgccgtca
      901 gcaggcccgg cgccgcgcgc aacaacggca agagagggcg cgcgtgctgc gggcgcatcg
      961 cgagctgcgc gagcacggcg gcgaagcgca cgcgcgcggc gaaatggcgc cgccactcgc
     1021 gcgcgtattc gcgcgcgacc gcggtttgcg ggccgccgcc ggagagaacg tcgcgctcgc
     1081 gcgcgagcgc ccggcacagc agccaagccg attgcatggc catgctgatt ccttcgccga
     1141 ggatcggatg cgcctcgccg gccgcgttgc cgaccgcgaa tcccgtgcgc tcgctccagg
     1201 ccgcgcgcat cgcgggccgc acgggcccga ccgagagcca ggggccctcg cgccgcgcgc
     1261 gctcgagcgc gaggcgcacg ccgcggcagc gagcggcgag cgccgcttgc acggcgtcgc
     1321 cggccgtcga ccgaggcgcg gccgcgcgcc attcccgcaa cgcgtcgcgg cgcacgcagc
     1381 aggcgagcgt cagcaggccg ccgtccgcga cgaccatgcc gccgtatccg ccgcggaacg
     1441 cgagcaccgg caacaggccg ggcgggaggc tcgcgccgct gaaattggcc ttgaacgcga
     1501 cgaggtcgct gcgccgcgcg cgccgggccg cgcattcctg ccacggctgc ggctcccacg
     1561 acccgtgcgc gccgatcagc accggcgcct cgatgcgccc ggcgtcgcgc tgctcgacgg
     1621 cgagccggca cgcgtaccgt gcgccgtgcc gctcgatctc cttgacggcg cacggctgcc
     1681 ggatcgcgac gccgagccgc gcagcgcgcg agagcagcag cgcatcgagc cggtcccggc
     1741 cgagcgcgcg cgcgaagccc gcgttcgcgc cctcgagccg cggcaggtct gcgcagatcg
     1801 tgtcgtcgcc gacgtagagc ccgacgcgct cgagctccgg tcccgcaagc tgcgcgaatt
     1861 ccgcgccgac ctcgagcgcg tcgagcagcg cgaggttcgg cgccgcgatg cattcgccgc
     1921 agaccttgcg gcgcgggaac gcgcgcttct cgacgagcgc gacggaccag ccggcctgcg
     1981 cgagcaagat cgcggcggtg cctcccgcga cgccggcccc gatcacgatc gcgtcgtagc
     2041 ggccggtcat ggcgcgcgcc tcgcgacgaa gagatggctg aacgcgccgg ccggcttctc
     2101 gtacgcggtc cagcctgctc cggccgcgcg ccgccactcg gcggtcagct cgcggtcgcg
     2161 gaaacccgcg tgcacgctga cgacggcatc gtggcgcgtg acgtcgttgc agccgatcgc
     2221 ccagagcaaa tggctaccgg cgagcgcgag acgcgcgcgc cgcggctcgc agcacacgaa
     2281 tgcggtcgcc tgcgcggcga cgcccgcgag caagcgcgcg agccgctcgc cttcgaagtg
     2341 atgcacgaac aggttcgcga ctacgacagc gtcgcggtcg ggccgggacc gctcgagcca
     2401 ctcgagcgcg tcggcggcga cgacgtcggc tgtccagccg aggtcgcgca gcgcggcgag
     2461 agttgcgtgc gtcacggtcg gctgcgcgtc gagcagcgtg accgcggcat gcggccagcg
     2521 gcgcgcgcgg cgccgcgcca atcgctcgag cagcgttccg tcgccggcgc cgagctcgac
     2581 gagccgcggc gccgggccgg tcatcacgtc gtcgagcgcg cgcgcgagca gagccgaatg
     2641 tcccatgatc cggttgacga ggcgcaggtc gcgccgcgag cgcagcgcgc gcgggtcgtc
     2701 gggtgggagc tcgtcgagca gctccgcgac gacgcggcgc ggccagcccg cttgcgccga
     2761 tgcagctcgc gccgccgcga tcatgcgacc tcgagcagcg cgccgtggca actgaagccg
     2821 gcgccgaacg acgacatcca ccaccaaccg ccgcgcgcgc ggtccgcaag ggccgcctcg
     2881 agcacgaaat agacgaacgc gctgctcaag ttgccgtact cgcgcaagat ccccgcgctg
     2941 tagcgcgtct gctcctcggt gagaccaagg cggctgcgca gcgcgagcag cacgtcgcgg
     3001 ccgcccgcgt gccaaaccca tgcggcgatc tcggcgcggt cgacaccggc gcggccgagc
     3061 acgtcgtcga gcacgcgctc agcatgcgac gccgcgagct tgggaacggg tcgcgtgagg
     3121 acgttgcgca gcatcccgct gcgctgctcg aagcgcaggg cttcgcgctc gacgggattc
     3181 aacagcgatc caatgctctt gcattcgatc gaacgcttat ccgcagccga acgccgtgac
     3241 agcactgccg cgcccgcgcc gtcaccgaat aggcacgcgc tgacgagaac gccgggatcg
     3301 ttgtcgaggt acatcgctgc gctgctgacc tcgacgcaca cggagaggac gttttcggag
     3361 cgccgccccg cgagcagtgc gtcggcgatc tgccagttgg gcagcgccgc ggcgcagccg
     3421 tgcccgacga gatcgaacgc ggcgaggtcg gggcgcagcc cgagccgctc gatgacatat
     3481 ccgctcaacc cggggcagag gtatcccgtg caagtgctca cgacgatcgc gtcgatgtcg
     3541 cggcgttcca ggttcgcgtc gc
//
LOCUS       DASSNN010000328         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_801391, whole genome shotgun sequence.
ACCESSION   DASSNN010000328 DASSNN010000000
VERSION     DASSNN010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801391"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VFL84_05990"
     CDS             complement(<1..1220)
                     /locus_tag="VFL84_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7608208.1"
                     /translation="MTKAQVLFASLIGTTIEFFDFYIYGTAAVLVFPYLFFPPDNPRT
                     ATLQSLATFALAFVARPVGSALFGHFGDRVGRKATLVAALLTMGLSTVAIGLLPTYAS
                     VGIAAPALLALCRFGQGVGLGGEWGGAVLLATENAPRGKESWYGMFPQLGAPLGFFCS
                     TGIFVALTAALTADEFLAWGWRIPFLASAALVGVGLWVRLTLTETPAFQRAVERAERV
                     RVPILTVVREHTRTLILAMLASVTTFVVFYVMTVFTLNWGTAGLGFSRQDFLELQMIG
                     VLSFALTIPLTAAAADRVGRWPILIGSSVAAILFGLTFAPLFGSGDKGTTLLFLSLGL
                     ALMGWTYGALGSALAELFPTAVRYTGASLTFNLAGILGGSLAPYIASDLAASHGIAYV
                     GYYVSAAAAVTLAA"
     gene            1332..>2228
                     /locus_tag="VFL84_05995"
     CDS             1332..>2228
                     /locus_tag="VFL84_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET7608209.1"
                     /translation="MEDLLSLDRLLAVWNAVYGWLRANVFVLDTAIQVGIVALTLLLA
                     VLLAKRLRVWLARYRDHRHVGRGVVIVESLALAIIWFGLLWIATTIVGLGERPNGLLR
                     TAATLLAAWIAIHLASQFVRNRAWAKLIAWAAWSLAALSIVGLLEPTIAVLDAAAVPI
                     GGDSRISLYDVVRSTIALAVLLSVALYLASLLEARIRTSQTLSPTVQVLFTKSLKMVL
                     VSLAVIFAIDAVGIDLTGLAVLGGLAVVAVGFGLQKIVSNLISGVVLLLDKSIKPGDI
                     ISVAGTYGWVTALGVRYVSVVTR"
BASE COUNT          371 a          739 c          764 g          354 t
ORIGIN      
        1 tgcgccgcga gcgtcacggc ggccgccgcg gacacgtagt agcccacgta cgcaatgccg
       61 tggctcgcgg cgagatcgct tgcgatgtac ggcgccaacg agccgccgag gatgccggcc
      121 aggttgaacg tcaacgatgc gcccgtgtaa cggaccgcgg tcgggaacag ctccgcgagc
      181 gcggagccga gcgcgccgta cgtccagccc atcagtgcga gcccgagcga gaggaacagc
      241 aatgtcgtcc ccttgtcgcc gctgccgaac agcggcgcaa aagtaaggcc gaacaggata
      301 gcggcgacgc tcgagccgat gagtattggc cagcggccga cgcgatcggc ggccgccgcc
      361 gtcaacggaa tcgtcagcgc gaaagagagc acgccgatca tttgcagctc gaggaagtcc
      421 tggcgcgaga agccgaggcc ggcggtgccc cagttcagcg tgaacaccgt catcacgtag
      481 aacacgacga acgtcgtgac ggaggccaac atcgcgagaa tcagcgtgcg cgtgtgctcg
      541 cgcacgacgg tcaagatcgg cacgcgtacg cgctccgcgc gctcgaccgc tcgttgaaac
      601 gcgggcgtct cggtcagcgt gagccggacc cacaggccta cgccgacgag cgccgcgctc
      661 gcgaggaacg ggatgcgcca gccccaggcg aggaactcgt ccgcggtcag tgccgccgtc
      721 agcgcgacga agattcccgt cgagcagaag aagccgagcg gcgccccgag ctgcgggaac
      781 atgccgtacc agctttcctt gccgcgcggc gcgttctccg tcgcaagcag cacggcgccg
      841 ccccattcgc cgccgagccc tacgccctgg ccgaaacggc agagcgcgag cagcgcgggc
      901 gccgcgatgc cgacggaggc gtaagtcggc agcaggccga tcgcgactgt cgacagtccc
      961 atcgtcagca gagcggcgac gagcgtcgct ttgcgcccga cgcggtcgcc gaagtggccg
     1021 aacagcgccg agccgacggg gcgcgcgacg aacgcgagcg cgaacgtcgc gagcgactgc
     1081 agcgtcgccg tccgcgggtt gtcggggggg aagaacagat acggaaaaac gagcacggcg
     1141 gccgtgccgt agatgtagaa atcgaagaac tcgatcgtcg tgccgatcaa gctcgcgaaa
     1201 agcacttggg ccttcgtcat tggggccggg attatatcgg cgcgctcgcc gctccggcga
     1261 ctgcgtttgg ttcagggctc gccgcgttcg ttagactcac ggtttcgcct caacctgcgg
     1321 aggtcacgta aatggaagac ctgctctcgc tcgaccgcct gctggcggtc tggaatgccg
     1381 tctacgggtg gctgcgcgcg aacgtattcg tgctcgacac cgcgatccaa gtcggcatcg
     1441 tcgccctgac gttgctgctc gcggtgctgc tcgcgaagcg cctcagggtt tggctggcgc
     1501 gctaccgcga tcaccggcac gtcggccgcg gcgtcgtcat cgtcgagtcg ctggcgctcg
     1561 cgatcatctg gttcgggctg ctgtggatcg cgacgacgat cgtcggcctc ggcgagcggc
     1621 caaacgggtt gctgcggacc gccgcgacgc tgctggctgc ctggatcgca atccatctcg
     1681 cgtcgcagtt cgtgcgcaat cgagcctggg ccaagctcat cgcctgggcc gcctggtcgc
     1741 tcgccgcgct cagcatcgtc ggccttctcg agccgacgat tgccgtgctc gacgccgcgg
     1801 ccgtcccgat cggcggagat tcgcgaatct cgctctacga cgtcgtacgg tcgacgatcg
     1861 cgctcgccgt gctgctgtcg gtggcgctgt acctcgcgag cctgctcgaa gcgcgcatcc
     1921 gcacgagcca gacgttatcg ccgacggtgc aggtgctgtt cacgaagtcg ttgaagatgg
     1981 tgctcgtgtc gctggccgtg atcttcgcga tcgatgccgt cggcatcgac ctgacgggcc
     2041 tcgccgtgtt gggcggcttg gccgtcgttg cggtcggttt cggcttgcag aagatcgtca
     2101 gcaacttgat cagcggcgtc gtcttgctgc tcgacaagtc gatcaagccc ggcgacatca
     2161 tctccgtggc cggaacctac ggctgggtca cggcgctcgg cgtccgctac gtatcggtcg
     2221 tgacgcgc
//
LOCUS       DASSNN010000329         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_190027, whole genome shotgun sequence.
ACCESSION   DASSNN010000329 DASSNN010000000
VERSION     DASSNN010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190027"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2457
                     /locus_tag="VFL84_06000"
     CDS             <1..2457
                     /locus_tag="VFL84_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7608210.1"
                     /translation="RPELRDLVVFASHDVVRDFPFSQLDLVACRRSFLDDLLPEVRRT
                     VLRNFHYALRPHGLLVVDGSVGLDVEASRLFSPDGDGGVYRRLAAPRHSFSPAAPLFA
                     PASRAGARAAGAKPRVIDARLVHLAMLERYAPASVLVDAENRVIHYSARAGRFLRLPG
                     GELTHELVHLLREPLRSAVRGGLEAVDREPAKWTSDALLVHTEHGVSRVAVHVEPAEG
                     DAAATRAKVVVFEEVQAGEPLADDDHARSASDYTSLLESGLEEAGRRLRAVVSQGKDA
                     GDGGTGGNEHDWELVHVLGDIESAKEELRTVNEELLSLNRVNRSRVEELARLSADLEV
                     LLESTGLATLFVDRDLKVVRFTSPLLEIFQVAPSDKGRPLAELKHRLSNDDLVSDAQR
                     VLNHLATIDREVEAHNGKWYLLRMLPYRSAPQGLGGVAITFVDITTRKKAELHLREAD
                     RRKDEFIALLAHELRNPLAPISSGIEILKRRDLDAGVAERVTMTMSRQAAQLVRLIDD
                     LLDVSRISSGRLRLRKTRVKLTDVVRDAVAAVRPMIERSGHQLELEAPREPIMLEADP
                     ARLTQVLANLLNNAARYTPSGGKISIAVRREGDAVVVSVKDTGYGIPEAALPHVFEMF
                     YQGADPRSATQTGLGIGLALAKSLVEMHGGAIHASSAGVDRGSEFTLRLPALAPVASD
                     DIPPEADVRAPLGGHRVLVVDDNADAAQTLAMLIQALGANDVHVALSGQEALPLAQRI
                     KPDTVFLDLKMPEMDGYEVAQRMRREPWGEATWLVALTGWGLDEHKRRTKDAGFDQHL
                     TKPADRAALEAILSRPAGRA"
     gene            complement(2491..2931)
                     /locus_tag="VFL84_06005"
     CDS             complement(2491..2931)
                     /locus_tag="VFL84_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608211.1"
                     /translation="MNRVSGRSRGVAHALLAATLACFVAPSLGAFAQDGAAPAAAAPS
                     AAPAEPAAGSRRDRRRAARPERAAAAESTATTTAAPAAMVEPEKICKNFKPLGSHMAK
                     RVCGTPEQWAAFEKKTTDAAADDMRNVRSTGAVIATTPGPSSTP"
BASE COUNT          453 a         1120 c         1135 g          485 t
ORIGIN      
        1 cgtcccgagc tgcgcgatct cgtcgtattt gcgtcgcacg acgtcgtcag ggattttccg
       61 ttctcgcagc tggacctcgt cgcgtgccgc cgcagcttcc tcgacgactt gctgcccgag
      121 gtcaggcgca ccgtgctgcg caatttccac tacgcgctgc ggccgcacgg cttgctcgtc
      181 gtcgacggca gcgtcgggct cgacgtcgaa gcgtcgcgct tgttttcgcc cgacggcgac
      241 ggcggcgtct atcgccgcct cgcggcgccg cgccactcgt tctcgccggc ggcgccgctg
      301 ttcgcaccgg cgagccgcgc cggcgcgaga gctgcgggag cgaagccgcg cgtcatcgat
      361 gcgcggctcg tgcacctcgc catgctcgag cggtatgcgc cggccagcgt tttggtcgac
      421 gcggagaaca gagtcattca ttactcggcg cgcgcgggcc gcttcctgcg cttgcccggc
      481 ggcgagctga cgcacgagct cgtgcacctc ttgcgcgagc cgctgcgcag cgcggtgcgc
      541 ggcgggctcg aagccgtcga tcgtgaaccg gcgaaatgga cgtccgacgc gctgctcgtg
      601 cataccgagc acggggtgag ccgcgtcgcc gtgcacgtgg agcctgccga aggcgatgcc
      661 gcggcgactc gggcgaaggt cgtcgtcttc gaggaagttc aagccggcga gccgctggcc
      721 gacgacgatc acgctcgctc ggccagcgac tacacgtcgc tgctcgagtc gggcctcgag
      781 gaggcgggca ggcgcctgcg cgccgtcgtc tcgcagggca aagacgccgg cgacggcgga
      841 accggcggca acgagcacga ctgggagctc gtgcacgtct tgggcgacat cgaatcggcg
      901 aaggaggagc tgcgaaccgt caacgaggag ctgttgtcgt tgaaccgggt caaccgctcg
      961 cgcgtcgagg agctcgcgcg cctgtcggcg gatctcgaag tgctgctcga gtccacgggt
     1021 ctcgccacgc tattcgtcga ccgcgacttg aaggtcgtgc gcttcacgtc gccgctgctc
     1081 gagatctttc aggtcgcgcc gagcgacaag ggccggccgc tcgccgagct caagcaccgg
     1141 ttgtcgaacg acgatctcgt gtccgacgcg cagcgcgtgc tcaaccactt ggcgacgatc
     1201 gatcgcgagg tcgaggccca caacggcaag tggtatttgc tccgcatgct gccgtatcgc
     1261 tccgcgccgc aagggttggg cggcgtcgcg atcacgttcg tcgacatcac gacgcgcaag
     1321 aaagccgagc tccatctgcg tgaagcggac cgccgcaagg acgagttcat cgcgctgctc
     1381 gcgcacgagc tgcgcaatcc gctcgcgccg atcagctccg gaatcgagat actgaagcgc
     1441 cgcgacctcg acgccggcgt cgcggagcgc gtcacgatga cgatgtcgcg ccaagccgcg
     1501 cagctcgtgc ggctcatcga cgacttgctc gacgtgtcgc ggatcagcag cggcaggctg
     1561 cggctgcgca agacgcgcgt gaagctcacc gacgtcgtcc gcgacgccgt cgcggccgtc
     1621 cggccgatga tcgagcgctc cggccatcaa ctcgagctcg aagctccgcg cgagccgatc
     1681 atgctcgagg ccgacccggc gcggctcacg caggtgctcg cgaatctgct caacaatgcg
     1741 gcgcgctaca cgccgtcggg cggcaagatc tcgatcgccg tgcgccgcga gggcgacgcc
     1801 gtcgtcgtgt ccgtcaagga cacgggctac ggcattcccg aggccgcgct gccgcacgtc
     1861 ttcgagatgt tctatcaggg tgccgacccg cgctcggcga cgcagacggg cttgggcatc
     1921 ggcctcgcgc tcgcgaaatc gctcgtcgag atgcacggcg gcgcgatcca cgcctcgagc
     1981 gccggagtcg accgcggcag cgagttcacg ctgcgcttgc ccgcgctcgc gcccgtggcg
     2041 agcgacgaca ttccgccgga agccgacgtt cgcgcgccgc tcggcggcca tcgcgtgctc
     2101 gtcgtcgacg acaacgcgga cgccgcgcag acgttggcga tgctgattca ggcgctcggc
     2161 gcgaacgacg tgcacgtcgc gttgagcggc caggaagcgt tgccgctcgc gcagcgcatc
     2221 aaacccgaca ccgtatttct cgacctcaag atgcccgaga tggacggcta cgaagtcgcg
     2281 cagcggatgc ggcgcgagcc gtggggcgag gcgacgtggc tcgtcgcgct gacgggctgg
     2341 ggcctcgacg agcacaagcg ccgcacgaag gacgcgggct tcgaccagca cctcacgaag
     2401 ccggccgatc gcgccgcgct cgaagcgatc ctgagccgcc cggccgggcg cgcgtgacgg
     2461 ctgcgatcag cgatctgccg cggagagagc tcagggcgtc gagctaggtc ccggagtcgt
     2521 cgcgatgacg gcgcccgtgc tgcgcacgtt gcgcatgtcg tccgccgctg cgtcggtcgt
     2581 cttcttctcg aacgcggccc actgttcggg cgtgccgcag acgcgcttcg ccatgtggct
     2641 gccgagcggc ttgaagttct tgcagatttt ttcgggctcg accatcgccg ccggagccgc
     2701 cgtcgtcgtt gccgtcgatt cggcggcggc cgcgcgctcg ggccgcgccg cgcgccggcg
     2761 gtcacggcgg gatcccgccg cgggttcggc cggcgcggcg ctcggtgccg cggcggccgg
     2821 cgcagcgccg tcttgggcga atgcgccgag cgacggcgca acgaagcacg cgagcgttgc
     2881 ggcgagcagt gcatgagcga cgccgcgcga gcgaccagag actcggttca tgtcattccc
     2941 ccttgtgcgg ccggcgcgcg acgttcgatc gctgcgtcgt tcgccgatgt tggcgcggga
     3001 taccgggtga gcgcaatgcc caagccatcc gtgccttctc gttagcggcg cgcgagcctt
     3061 cgcctcgcga gcagcgccgg gatcagcagc agcgcgagcc agcccatcgc gccggcaccg
     3121 ccgtcttcga gcggcgcctc cgcgggcggc gctgccggcg gtgccgccgc cgcgggcggc
     3181 ggagcgggcg ccg
//
LOCUS       DASSNN010000330         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_438053, whole genome shotgun sequence.
ACCESSION   DASSNN010000330 DASSNN010000000
VERSION     DASSNN010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438053"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="VFL84_06010"
     CDS             complement(<1..1402)
                     /locus_tag="VFL84_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HET7608212.1"
                     /translation="MIDVVSSSPQQDRDRGSVEWRALRGLAPHLWPSGDVRVRLRVVL
                     ALALLAAAKAATVYVPILLKKLVDLFADPANLPVIVPLGLVVGYGILRVAAIAFAELR
                     DAVFAKVAQRAIRTVALQTFRHLHALSLKFHLERQTGGLSRAIERGTNGIDTLLTFML
                     FNIVPTLIEIALVCGILWSFFGWTYALVTFICVVGYAAYTFLVTEWRIKYRRQMNETD
                     QEASTRAIDSLLNYETVKYFGNEQYEANRFDQALARYERASVASKSSLSLLNIGQAAI
                     IGTGLAVLMLMAGRGVIAGSLTVGDFVLVNTYLIQLYIPLNFLGFVYREIKQALTDME
                     SMFRLMGVAADVQDLPGARPLRAGPGEIVFDHVSFAYDPRRTILHDVSFRVPAGGRVA
                     IVGASGAGKSTISRLLFRFYDVTSGAIRIDGQDIREATQDSVRAAIGIVPQDTVLFND
                     TIYYNIAYGRPGASPAE"
     gene            1505..>2385
                     /locus_tag="VFL84_06015"
     CDS             1505..>2385
                     /locus_tag="VFL84_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608213.1"
                     /translation="MTKGDLVVSTEMGGLGNRMKSWVSSMRLSADARVLWPAQKMPAG
                     FGELFANDCAVDAVPEGARVYNSWRLAILPEDEAHLPAGFATVGAGAHPIVRGLGKAW
                     WSLTGERTDRYRFMVFPKQHSRRSTRADARHIDLEYERIPDHFRALYAELFRRILVRP
                     EILAAASDWAAANLDETVVGVHVRTWRDDPRRYRKYHVPSLRRLHRLLESTGNARFLV
                     VSDSDDVAPMLAERYGARRVIGYPRTTPRADSWLTPRGVQEDLIDMLLLARTQRLLAS
                     YLSTFSEVAWWLGGTKAR"
BASE COUNT          418 a          769 c          822 g          376 t
ORIGIN      
        1 cctccgccgg tgaagctccg ggccggccgt acgcgatgtt gtagtagatc gtgtcgttga
       61 agagcacggt gtcctgcgga acgatgccga ttgcggcgcg aacgctgtcc tgggtcgctt
      121 cgcggatgtc ttgaccgtcg atgcggatgg cgcccgaggt cacgtcgtag aagcgaaaca
      181 acagccgcga gatcgtcgac ttgccggcgc cgctcgcgcc gacgatcgcg acgcggccgc
      241 cggccggcac gcggaacgag acgtcgtgca ggatcgtgcg gcgcgggtcg tacgcgaagc
      301 tgacgtggtc gaagacgatc tcgcccgggc cggcgcgcag cggccgcgcg cccggcaagt
      361 cctggacgtc tgcggctacg cccatcaagc ggaacatcga ctccatgtcc gtcagcgcct
      421 gcttgatctc gcgatagacg aaccccaaga agttgagcgg aatgtacagc tggatcagat
      481 acgtgttgac gagcacgaag tcgccgaccg tcaacgatcc ggcgatgacg ccgcggcccg
      541 ccatcagcat cagcacggca agccccgtac cgatgatcgc ggcctggccg atgttcagca
      601 gcgacagcga gctcttgctc gcgaccgacg cgcgctcgta gcgcgcgagc gcctggtcga
      661 agcgattcgc ttcgtattgc tcgttgccaa agtacttcac cgtctcgtag ttgagcaggc
      721 tgtcgatcgc gcgcgtgctc gcttcctggt cggtctcgtt catctggcgg cgatacttga
      781 tgcgccattc cgtgacgaga aacgtgtagg ccgcataacc gacgacgcag atgaacgtca
      841 cgagcgcgta ggtccagccg aagaagctcc agagaatgcc gcagacgagc gcaatctcga
      901 tcagcgtcgg cacgatgttg aacagcatga aggtcagcag cgtgtcgatg ccgttcgtgc
      961 cgcgctcgat cgcgcgggaa aggccgcccg tctggcgctc gaggtggaat ttcagcgaca
     1021 gcgcgtgcag atgccggaac gtctgcagcg cgacggtgcg gatcgcgcgc tgcgcgacct
     1081 tcgcgaagac ggcgtcgcgc agctccgcga atgcgatcgc ggccactcgc aagatgccgt
     1141 agccgacgac gaggccgagc ggcacgatca ccggcaggtt cgcgggatcc gcgaatagat
     1201 cgacgagctt cttcagcaag atcggcacgt agacggtggc ggccttcgcc gcggcgagca
     1261 gcgcaagcgc cagcacgacg cgcaggcgca cacgcacgtc gccggagggc caaagatgcg
     1321 gcgcaaggcc gcgcagagcg cgccactcga cgctgcctcg gtcgcggtct tgctgcgggg
     1381 atgagctcac gacgtcgatc atcgccgacg ccgatcgggc cgtccacgtg aatgcggcgc
     1441 cggccgcggg taagatcgcc gcgattcgat gagcgcgcaa cgatgactga cagaacagca
     1501 accgatgact aagggcgatc tcgtcgtatc caccgaaatg ggtgggctcg gcaatcggat
     1561 gaagtcgtgg gtgtcgagca tgcgcctctc ggctgacgcg cgcgtgctct ggccggcgca
     1621 gaagatgccg gccggtttcg gcgagctgtt cgcgaacgac tgtgccgtgg acgccgtgcc
     1681 ggagggcgcg cgcgtataca actcgtggcg cctcgcgatc ttgccggagg acgaagcgca
     1741 cctgccggca ggctttgcga cggtcggcgc gggagcgcac ccgatcgtcc gcgggctcgg
     1801 caaggcgtgg tggagtctca cgggcgagcg aaccgatcgc taccgcttca tggtgttccc
     1861 gaagcagcac tcgcggcgtt cgacgcgcgc cgacgcgcgg cacatcgacc tcgagtacga
     1921 acggataccg gaccattttc gcgctctgta cgccgagttg tttcggcgca tcctggtccg
     1981 gcccgagatc ttggcggcgg cgagcgactg ggccgccgcc aacctcgacg agacggtagt
     2041 cggcgtccac gtgcgcacgt ggcgagacga tccgcgccgg taccgcaagt accacgtgcc
     2101 gtcgctgcgc cggctccatc gcttgctcga gtcgaccgga aacgcgcggt ttctcgtcgt
     2161 cagcgacagc gacgacgtcg cgccgatgct ggcggagcgg tacggcgcgc gccgcgtcat
     2221 cggctacccc cgcacgacgc cgcgagccga cagctggctg acgccgcgcg gcgtccaaga
     2281 ggatctcatc gacatgctgc tcttggcgcg cacgcagcgt ttgctggcga gctacttgag
     2341 cactttcagt gaagtggcct ggtggctcgg cggaacaaag gcgcg
//
LOCUS       DASSNN010000331         3186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_190276, whole genome shotgun sequence.
ACCESSION   DASSNN010000331 DASSNN010000000
VERSION     DASSNN010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190276"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFL84_06020"
     CDS             <1..212
                     /locus_tag="VFL84_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018897626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HET7608214.1"
                     /translation="HAYGGTRMGDNPETNVVDRWGFCHEVPNLGVLGASVMGTSGARN
                     PTLTAQALAWRTAERLVADWRSIAE"
     gene            246..830
                     /locus_tag="VFL84_06025"
     CDS             246..830
                     /locus_tag="VFL84_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608215.1"
                     /translation="MTASKTIRVDRLCALCAIAACFAAMGQGPQPSLSVEADSVTFDL
                     RSNSSVSKDVTISDGRVRIEAGEGRSTGGETGDGSWELRGGVRIAIDSARMSGDAATF
                     RYSGGQVTFGEITGDPVEFESPGATATAVRGTAGKISFDRAQAVLAATGNASFVMGAQ
                     EVDCDWTFNLVDRTASGSGEGDDKCVVRIPIRRR"
     gene            1000..2247
                     /locus_tag="VFL84_06030"
     CDS             1000..2247
                     /locus_tag="VFL84_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7608216.1"
                     /translation="MTTVSRGLPPRPHLDVPKRLARELLAAWRSRERDAFDRIRRQHP
                     KFEAADDAAVAAGPFRLSDAQLVIAREYGLKHWTELKQRIDANTPAQALAAAIRADDR
                     AAAVEIARREPQLLHIPLVSGNWGPPMSHAANLGRLEMVKAMAALGAKDFQLAFGRAV
                     LQGRVDCARWLLGHGAELDPGAAMGACEALNSSGLRFIAELGGPITDERGSKLAPLAM
                     ILQTYSRDPQGKHEMLELLAQRGYELPDTPIMAFHRGDIARLEKHLRSDPRLLERRFT
                     LADIYPAECGCDGSGMHWTPIDGATLLHLAFDFHETAIAEWLLARGADANARASVDAD
                     GFGGHTPLFNAVVCGPWHHDKPARLLLQYGAAKDPRASLRKFLDWVEEPRWHEARDVT
                     ALQWARGFPDQGWVNGKALRMLE"
     gene            2424..>3186
                     /locus_tag="VFL84_06035"
     CDS             2424..>3186
                     /locus_tag="VFL84_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7608217.1"
                     /translation="MLAALGAGCSEPAQPPETPPLVEVATPLAQRVTDWDDYSGRFEP
                     VDAVEVRPRVSGAIESVHFTDGQVVDQGQLLFVIDPRPYAAELAHAKAEVAGARAQLA
                     NADAELKRAQALAGNKLISEADVEVRVAAQLRAAADLAAAEASVQSADLNLSFTRVTA
                     PLAGRASYRRLAPGNIVSADTTVLTTIVTEDPIRFLFDVPESALLKYKREADGATASR
                     VEIRLQDETEYRWPGRVDFRDNALDRGSGTIRLRAV"
BASE COUNT          528 a         1070 c         1091 g          497 t
ORIGIN      
        1 cacacgcgta cggcggcacg cgcatgggcg acaacccgga gacgaacgtc gtcgaccgct
       61 ggggcttctg ccacgaggtg ccgaatctcg gcgtgctcgg cgcgtcggtg atgggcacga
      121 gcggcgcgcg caatccgacg ctgacggcgc aagcgctcgc gtggcgcacg gccgagcgtc
      181 tcgtagcgga ttggcgctcg atcgccgagt aacgacattc agtggacacg cgaggagctc
      241 gagccgtgac ggcgtccaag acgattcgcg ttgatcgact ctgtgcactg tgcgcgatcg
      301 ccgcgtgttt cgcggcgatg gggcaggggc cgcagccaag cctctccgtc gaggcggatt
      361 ccgtgacttt cgacttgcga tccaacagca gcgtctccaa agacgtcacg atctcggacg
      421 gaagagtccg catcgaggcc ggcgaaggcc gcagcacggg cggcgagacc ggggacggga
      481 gctgggagct ccgcggcggc gtacgaatcg cgatcgattc cgcgcgtatg tccggagacg
      541 cggcgacgtt ccgctactcg ggaggtcaag tcacgttcgg cgagatcacc ggcgacccgg
      601 tcgagttcga atcgcccggc gcaaccgcca ctgcggtgcg cgggacggcc ggcaagatct
      661 ctttcgatcg ggcgcaggct gtccttgccg cgactggaaa cgcatcgttc gtcatgggcg
      721 cgcaagaagt cgattgcgac tggactttca acctcgtcga cagaacagca agcggcagtg
      781 gcgagggtga cgacaagtgc gtcgtgcgaa tcccgattcg cagacgttag gccccagcgg
      841 ttgctcggct cacgccgttc gtccatgcta acggcgctcg ccggaagcac cccgtccgcg
      901 gaaatgggcc tagcccaccg gtcgtctggc ttcgacaatt caacctgact tcctgcccaa
      961 tgcgtgcgga ccaagggcgc cttctgagga ggttcggttg tgaccaccgt atctcggggc
     1021 ttgcccccac gccctcatct cgacgttccg aagcgcttgg cgcgcgagct gctcgccgcg
     1081 tggcgcagcc gtgagcgcga cgcgttcgac cggatccgcc ggcagcatcc gaagttcgag
     1141 gccgccgacg atgccgccgt cgccgcgggc ccgtttcgct tgagtgacgc gcagctcgtc
     1201 atcgcgcgcg agtacggcct caagcattgg acggagctca agcagcgcat cgacgcgaac
     1261 acgcccgcgc aggcgttagc ggccgcgatc cgcgccgacg atcgcgccgc cgcggtcgag
     1321 atcgcccgcc gagaaccgca gttgctgcac attccgctcg tcagcggcaa ttggggaccg
     1381 ccgatgagcc acgccgcgaa tctcggccgc ctcgagatgg tcaaagcgat ggccgcgctc
     1441 ggcgcgaagg attttcagct cgcgttcggc cgcgccgtgc tgcagggccg ggtcgattgc
     1501 gcgcgctggc tactcgggca cggtgcggag ctcgatccgg gcgctgcaat gggagcgtgc
     1561 gaggcattga attctagcgg cctgcgcttc atcgccgagc tcggtggccc gatcacggac
     1621 gagcggggat ccaagctcgc gccgcttgcg atgatccttc aaacgtacag ccgcgatccg
     1681 cagggcaagc acgagatgct cgagctgctc gcgcagcgcg gctacgagtt gcccgacacg
     1741 ccaatcatgg cgtttcatcg cggcgacatc gcgaggttag agaagcacct gcgcagcgat
     1801 cctcgcttgc tcgaacgcag gttcacgctc gccgatatct acccggcgga atgcggctgc
     1861 gacggcagcg gtatgcactg gactccgatc gacggcgcga cgctgctgca cctcgccttc
     1921 gatttccacg agacggcgat cgccgagtgg ctgctggctc gaggcgcgga cgccaacgcg
     1981 cgcgcctcgg tggacgccga cggcttcggc ggtcatacgc cgctcttcaa cgccgtcgtt
     2041 tgcggacctt ggcaccacga caaaccggcg cggctgctgc tgcaatacgg cgccgcgaag
     2101 gatccgcgcg cgagcttgcg caagtttctc gactgggtcg aagagccgcg ctggcacgaa
     2161 gcgcgcgacg tgacggcgct tcagtgggcg cgcggatttc cggatcaggg ttgggtgaac
     2221 ggcaaagcgt tgcgcatgct cgagtaacga gccgcggccg cgcgtgccaa acggccgcgc
     2281 cgaccgcact tcttgccgcc gcaagagacc gcccggtacg gcgacccaat gtgcgtcgag
     2341 agtcacattc gcgctattgt gacaacgtcg tttcgccaaa ctgccactcg atgccgagaa
     2401 ggaaatcgat ctcgcttacc gccatgctcg ccgcgctcgg cgcgggttgc agcgagcccg
     2461 cgcaaccgcc ggaaacgccg ccgttggtgg aggtcgcgac acctctcgcg cagcgcgtga
     2521 cggattggga cgactactcg gggcgtttcg agcccgtgga tgcggtggaa gtacggccgc
     2581 gagtgtcggg cgcgatcgag tccgtgcact ttacggacgg ccaggtggtc gatcaaggtc
     2641 agttgctgtt cgtcatcgat ccccgcccgt acgccgcaga gctcgcgcac gccaaagccg
     2701 aggtcgccgg cgcgcgcgcc cagctcgcga acgccgatgc cgagctcaag cgcgcgcaag
     2761 cgctcgcggg caacaagctc atcagcgagg cggacgtcga ggtccgcgtc gccgcgcagc
     2821 tgcgcgcagc cgcggacctc gcggccgccg aggcgtcggt gcagagcgcg gacttgaatc
     2881 tgtcgttcac gcgcgtcacg gcgccgctcg cgggccgcgc gtcgtaccgc cgcttggcgc
     2941 ccggcaacat cgtcagcgcc gacacgacgg tgctcacgac gatcgtcacc gaggacccga
     3001 tccgtttcct gttcgacgtg ccggaatccg cgctgctcaa gtacaagcgc gaggcggacg
     3061 gcgcgacagc gagccgcgtc gagatccgcc tccaggacga gaccgagtat cgttggccgg
     3121 gccgcgtgga ttttcgcgac aacgcgctcg accgcggctc cggcacgatt cggctgcgag
     3181 ccgtcg
//
LOCUS       DASSNN010000332         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_295227, whole genome shotgun sequence.
ACCESSION   DASSNN010000332 DASSNN010000000
VERSION     DASSNN010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295227"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..558)
                     /gene="pal"
                     /locus_tag="VFL84_06040"
     CDS             complement(34..558)
                     /gene="pal"
                     /locus_tag="VFL84_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HET7608218.1"
                     /translation="MRSAFLILLAAVTLTLTACGRDRTQPETTAPADTGANTNNDLST
                     ERIGGGGDVSASALSARDRELVRTLVIYFDYDQADIKPEFNELLAAHGRQLVANPNMN
                     LRLEGHTDERGSREYNIGLGERRAQAVRRVLTLQGVSAAQLTTVSYGEERPAQTGSDE
                     ESWRLNRRVELVYR"
     gene            complement(626..1984)
                     /gene="tolB"
                     /locus_tag="VFL84_06045"
     CDS             complement(626..1984)
                     /gene="tolB"
                     /locus_tag="VFL84_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HET7608219.1"
                     /translation="MQRGLESNARSEWLGCVRVPLLCLAAAFGAWLAPGAKAQLNVTI
                     VAGSERAVPIAVVPFGWQGSGPPAFDIAGVVSSDLGSSGRFAPIANRDMVSRPTQDTQ
                     VNFQDWRLLRTSYLVIGTLAETAPDNFEATFSLFDVVRGERLTAFRLQAARADLRRAA
                     HRISDMVFEELTGFPGVFSTQIAYVSEERRPDGSRLFRLIVSDADGENANKVAESTQP
                     LLSPSWSPDARRIAYVSFEGGVSGVYVQTLRTGTRERVSQRAGVNSAPTFSPDGRFLA
                     LVLSRDTGNLDIHTLDLQTQVLRQLTTDAGIDTEPAWSPDGRTIYFNSDRSGGPQIYR
                     VGTEAGQRATRITFDCVYCARPRVSPDGKLIALVNGENNRYRIAVWDIEAQRLDLLTD
                     GRLDESPSFAPNGAQIIYATMGQNGRGVLASVSTDGRFKTEIASVAGNVREPVWGPFP
                     RP"
     gene            complement(1984..2766)
                     /locus_tag="VFL84_06050"
     CDS             complement(1984..2766)
                     /locus_tag="VFL84_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HET7608220.1"
                     /translation="MRPVAVIWSSVLHVALLVAVIVGLPWQFERPRPAIVAAPIQGVI
                     IDQAAIERERKKQEDAARQARLQQQREDRQRREADEQRRLAEQREQQRVADEKRAREE
                     AQRKEEQRLAAERAEQQRKKQEAEAQARREREEAEKRAAEQRRREQAESELQAQLRAE
                     AEANAAREAGLLEQYILMIEDKIKEKWDRPLSARPGIDCVVSVVQLPTGDVVSARVAR
                     CNGDDAVRRSIENAVMDASPLPKPPHPSLFERNLNVNFKPEQ"
     gene            complement(2776..3231)
                     /gene="tolR"
                     /locus_tag="VFL84_06055"
     CDS             complement(2776..3231)
                     /gene="tolR"
                     /locus_tag="VFL84_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016389859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HET7608221.1"
                     /translation="MNTTMSRSKGKRRLMSDINVVPYIDVMLVLLIIFMITAPLLTQG
                     ITVDLPNAPASQLDPGQNEPLVLSVDREGKYYLNVGDDEDMPIDDETVVERASAVLRR
                     NAETPVLVQADETVPYGRVVTGAVLLQQAGAAKIGFQTDPGGAERDRRR"
     gene            complement(3228..>3409)
                     /locus_tag="VFL84_06060"
     CDS             complement(3228..>3409)
                     /locus_tag="VFL84_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608222.1"
                     /translation="AIPAGIFYNRYADRVGRLEVRYDAFMEELSAIFQRNAVRRDAAS
                     RGAGPVSGELAEIET"
BASE COUNT          508 a         1176 c         1127 g          598 t
ORIGIN      
        1 acggcgcgcg gcggcggccc gccgcgcgat cactcagcga taaacgagct cgactcgccg
       61 gttcaggcgc cacgattcct cgtcgctgcc cgtctgcgcg ggacgctcct cgccgtagct
      121 gaccgtcgtc agctgagcgg cggatacacc ctgcaacgtc agaacgcggc gcacggcctg
      181 cgcgcggcgc tcgccgaggc cgatgttgta ctcgcggctg ccgcgctcgt cggtatggcc
      241 ttcgagccga agattcatgt tcggattcgc gacgagctgt cgaccgtgcg cggcgagcag
      301 ctcgttgaac tcgggcttga tgtcggcctg gtcgtagtcg aagtagatga cgagcgtcct
      361 tacgagctcc cgatcgcgcg cgctgagcgc cgaggcgcta acgtcgccgc cgcccccgat
      421 gcgttcggtc gacaggtcgt tgttcgtgtt tgcacccgta tcggccgggg cggtcgtttc
      481 aggctgcgtc cggtcacgac cacaggccgt cagcgtcaac gtcactgcgg ccagcaagat
      541 caagaatgcg cttctcatct gggtaatcct cggagtcaag tgaaaattag ggcgatagga
      601 tactgcaagg cactggtctc gtttttcacg gccgcgggaa cggtccccac acgggctcac
      661 gcacgttgcc cgcaaccgat gcgatctcgg tcttgaaccg gccgtcggtg ctcaccgatg
      721 cgagcacacc gcggccgttc tggcccatcg tcgcgtaaat gatctgcgcg ccgttcggcg
      781 cgaagctcgg cgactcgtcg agacggccgt ccgtcaacaa gtcgagccgc tgcgcttcga
      841 tgtcccaaac ggcgattcga tagcgattgt tctcgccgtt cacgagcgcg atgagcttgc
      901 cgtcgggcga cacgcgcggc cgcgcgcagt agacgcaatc gaacgtgatg cgtgtcgcgc
      961 gctgcccggc ctccgtgccg acgcggtaga tctgcgggcc gcccgagcga tcggaattga
     1021 agtagatcgt gcggccgtcc ggcgaccagg ccggctcggt gtcgatgccg gcatcggtcg
     1081 tgagctgtcg caacacttgc gtctgcagat cgagcgtgtg gatgtcgagg ttgcccgtgt
     1141 cgcgcgacag cacgagcgcg aggaagcggc cgtccggcga gaacgtcggc gcgctgttga
     1201 cgcctgcccg ctgcgacacg cgctcgcgcg tgcccgtgcg cagcgtctgc acgtagacac
     1261 cggagacgcc gccttcgaac gacacgtacg cgatgcgccg cgcgtcgggc gaccaggacg
     1321 gcgacagcag cggctgcgtc gactccgcga ccttgttcgc gttctcgccg tcggcgtccg
     1381 agacaatcaa gcgaaacagc cgcgatccgt ccgggcggcg ctcctcgctg acgtacgcga
     1441 tctgcgtgct gaagacgccc gggaagcccg tcagctcctc gaagaccatg tccgagatgc
     1501 ggtgcgctgc gcggcgcaaa tcggctcgcg cggcctggag cctgaaggcc gtgagccgtt
     1561 cgccgcgcac gacgtcgaac agcgagaacg tcgcctcgaa attatcaggc gccgtctcgg
     1621 ccagcgtgcc gatcacgagg tagctcgtgc gcagcaggcg ccaatcctgg aaattgacct
     1681 gcgtgtcttg agtgggccgc gacaccatgt cgcggttcgc gatcggcgcg aaccgtccgc
     1741 tcgagcccaa gtcggagctg accacgcccg cgatgtcgaa tgccggcgga cccgagccct
     1801 gccagccgaa cggcacgacg gcgataggca ccgcgcgctc ggagcctgcc acgatcgtga
     1861 cgttgagttg cgctttggcg ccaggggcga gccatgcccc aaacgccgcg gccaagcaga
     1921 gcagcggcac cctgacgcag cccaaccact cgcttcgcgc gttcgactct aagcctctct
     1981 gcatcactgc tccggtttga aattgacgtt caagttgcgc tcgaacagcg aaggatgggg
     2041 cggcttcggc agcggcgacg catccatgac cgcgttctcg atcgagcggc gcacggcgtc
     2101 atcgccgtta catctcgcga ctcgcgcgct gacgacgtcg ccggtcggca gctgcaccac
     2161 cgagacgacg cagtcgatgc cgggccgcgc cgacagcggc cggtcccact tctccttgat
     2221 cttgtcctcg atcatgagga tgtattgctc gagcaatccc gcttcgcgcg cggcgttcgc
     2281 ctccgcctcg gcgcgcagct gcgcctgaag ctcggactcg gcctgctcgc ggcggcgctg
     2341 ctcggccgcc cgcttctcgg cttcctcgcg ctcgcggcgc gcttgcgcct cggcttcctg
     2401 tttcttgcgc tgctgctcgg ccctttccgc ggcgagacgc tgctcttcct tacgctgcgc
     2461 ctcctcgcgc gcgcgctttt cgtcggcgac gcgctgctgc tcgcgctgct cggcgaggcg
     2521 ccgctgctcg tccgcctcgc gccgttgccg atcttcgcgc tgctgctgga gccgagcctg
     2581 gcgcgccgcg tcttcttgct tcttgcgctc tcgctcgatg gccgcctgat cgatgatgac
     2641 gccttgaatc ggagcggcca cgatggccgg ccgcggccgc tcgaactgcc atggcaggcc
     2701 gacgatgacg gccacgagca gcgcgacgtg cagcacggac gaccagatga cggctacggg
     2761 cctcatggtt ttgtctcagc ggcgccgatc gcgctcggcg ccgccgggat cggtttggaa
     2821 gccgatcttc gcggcacccg cttgttgcag aagcacggca cccgtgacga cgcggccgta
     2881 cggcaccgtc tcgtcggctt gcacgagcac gggcgtctcc gcgttgcggc gcagcacggc
     2941 cgacgcgcgc tcgacgaccg tctcgtcgtc gatcggcata tcctcgtcgt cgccgacgtt
     3001 caagtagtac ttgccttcgc gatcgacgga cagcacgagc ggctcgttct ggccggggtc
     3061 gagctgcgac gccggcgcgt tcggcaagtc gacggtgatg ccctgcgtca ggagcggcgc
     3121 agtgatcatg aagatgatca ggagcacgag catgacgtcg atgtagggca cgacgttgat
     3181 gtcggacatc aagcgccgct tacccttgct cctgctcatc gtcgtgttca tgtctcgatc
     3241 tccgcgagct cgccggagac cggtcccgcg ccgcggctcg cggcgtcgcg ccgcacggcg
     3301 ttgcgctgga agatcgccga cagctcttcc atgaacgcgt cgtaccggac ctcgaggcgg
     3361 ccgacgcgat ccgcgtaccg gttgtagaag ataccggccg ggatcgccg
//
LOCUS       DASSNN010000333         6111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1809379, whole genome shotgun sequence.
ACCESSION   DASSNN010000333 DASSNN010000000
VERSION     DASSNN010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809379"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VFL84_06065"
     CDS             <1..805
                     /locus_tag="VFL84_06065"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7608223.1"
                     /translation="IEPDNRSWQTGLLQALNETQQHAAGLALVEQMLQAEPDDAVLWL
                     YRAHMSINSGQRGSALTSLEMAMRLGDDSVANKQVAAALHMERGSIGRAVELLKSAAA
                     DDLDFQFIDQALARLNYEDEWDYFRDLLASAEQRARFLDNLQGSRLLTQRASLQLHDG
                     NRRQAIASLQQAVELDASNAEALITLGQAYRDERDYNRAEVMFQRASAFELFRENALV
                     SLAGLAVDQQNFERALELLREVVSSNPARTELQRNVDVLENLVLERTRD"
     gene            812..2425
                     /locus_tag="VFL84_06070"
     CDS             812..2425
                     /locus_tag="VFL84_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7608224.1"
                     /translation="MKRWAQLWLSIMLAPAAHAAEIELTITPPEPEWLLYPELSPGPC
                     AFPGGFRLEACPKQLMPEGIANIGEQSLVIDLGPLLAAGNYEAALARLRLNFGPHLLL
                     LEAGDVDGFRRARLPTSGEGTLRPGPGSRGRDTPSMNFTMERSNVPTAEGAGDTPGRL
                     TGRSSRSGVAPDSISASMLYVIGHCYFSLQRYLPAETAFKLALVALPNHVRAHESLAI
                     LYLRTGRYADARVHLTKLAELGRNTAQVHSARGYLEQKTHRHWYAADAFQKALVLAPD
                     DRGAQRGLLLALTETHEYAKAKALVDRLLRDEPDDRDLWLYRAQIALSTDQRAVALAS
                     LETALALGEDSVENRRACFELQMENGNIARGVELLRGLRARDLPFPLVDQALGWLANE
                     SQWDRFREVLGSVDRERLDGVEQSRLLTQRAALAAHDGNRRAASTALQEALALDPSNA
                     DALIALGQIYRVDRDYGRADLLFQRASAYAAARESALVARADVASDEGNFDGALAALR
                     DAAALDPTRGDLKRNIEVLENLSLLRTQR"
     gene            2518..3135
                     /locus_tag="VFL84_06075"
     CDS             2518..3135
                     /locus_tag="VFL84_06075"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7608225.1"
                     /translation="MRPNNHYILPLLAALAASGVASAQRERTEPPVAEVEPAPNPSTS
                     ERVGPSDPGLALEVQARLYQALTDSNMSVLVRYGVATLDGVVRTEADRQRAEEIALQV
                     PGIDSVVNDLTVAPPIAIAAVDEAQAITAQENTKVEDQVAQQLRADATLGSRDIRVVA
                     DRLTNTITLSGTVSTEEEKERAGQIAVKAFPVGQVRNQLEVRQRL"
     gene            complement(3158..4483)
                     /locus_tag="VFL84_06080"
     CDS             complement(3158..4483)
                     /locus_tag="VFL84_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012484724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608226.1"
                     /translation="MSTLSRGFSTFAALAVTMAVGGARADDRPQPPGGDVWQHAATQI
                     ARGRNTFRFDTFGDEDFWGGALHLHESVAGAANGGVGPGLSPKAALELGLKVDVRALP
                     PPLVQALRAGKVNLDDPAVTLALLKRNAVVGVRAFTNRAGNVESVGITCALCHSTVDN
                     SFAPGIGARLDGWANRDLNVGEIIAFAPSVAPFAQLLGVDEATVRKVLRSWGPGKFDA
                     ELLLDGKAFRPDGKSAATLLPPAFGLAGVNLHTWTGWGSVTHWNAFVAVLEMQGKGTL
                     YDPRLEDASKFPIAAANDFGNTRREPDLVTPKLGALQLYQLAIAAPPPPKGSFDARAA
                     ERGKALFAADGKAHCATCHVPPIFTEPGHNLHKPEDIGIDAFQAQRSPTDGYRTSPLR
                     GLWTHTKGGFYHDGRFATLREVVDHYDSFQGLGLSNGEKRELVEYLKSL"
     gene            complement(4507..5301)
                     /locus_tag="VFL84_06085"
     CDS             complement(4507..5301)
                     /locus_tag="VFL84_06085"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7608227.1"
                     /translation="MNERPELGAQDGLAPNTVVDAAIPDAALVDARLAGPVTLEPPIP
                     EPGLSAAAIPDAVLADEAIVARVLTGDSALFELVMRRYNRLLFRLARGILRDDDEARD
                     VVQAAYVRAYYHLGQFRGPAGFKAWLARIAVNEALGRVRRAPPIVDAEQHALALTDLP
                     EAEPENAASSRDLLRILQAAIDTLPDEFRRVFMLRGVEQLSIAETAELLEINPATVKT
                     RFHRARRMLQEQLQRTLDDVVPTAFPFGGQHCDAIVGTVLARIRCQ"
     gene            complement(5735..>6111)
                     /locus_tag="VFL84_06090"
     CDS             complement(5735..>6111)
                     /locus_tag="VFL84_06090"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HET7608228.1"
                     /translation="RVVPRIIGYGRYVRPTLGVDSDDRVTQQLFGRDGAGGVLVLGVA
                     GGSPAERVGLRPTRQERNGNIIIGDIIAAVDGRPVETEADLIGVLDEHEFGDRVTLTV
                     IRNDRRIEVPVTLGTAGESRTR"
BASE COUNT         1021 a         2102 c         2065 g          923 t
ORIGIN      
        1 gatcgagccc gataatcggt cgtggcagac cggcctgctg caggcgctga acgagacaca
       61 gcagcacgcg gccggcctcg cgctcgtcga gcaaatgctg caagccgagc cggacgacgc
      121 cgtgctctgg ctgtatcgcg cgcacatgtc gatcaactcg ggccagcgcg gctcggcgct
      181 cacgagcttg gaaatggcca tgcggctcgg cgacgactcg gtcgcgaaca agcaggtcgc
      241 cgccgcgctg cacatggagc ggggcagcat cggcagggcc gtcgagctat tgaagagcgc
      301 ggccgccgac gacttggact ttcagttcat cgatcaggcg ctggcgcggc tcaactacga
      361 ggacgagtgg gactacttcc gcgatctctt ggcatccgcc gagcagcgcg cccgttttct
      421 cgacaatttg cagggaagcc ggctgctcac tcaacgcgcg agcttgcaac tgcacgatgg
      481 caatcgccgc caggcgatag cgtcgctgca acaagccgtg gagctcgacg cgtcgaacgc
      541 cgaggcgctc atcacgctcg gtcaggccta tcgcgacgag cgcgattaca accgcgcgga
      601 ggtcatgttc caacgcgcga gcgctttcga gctcttccgc gagaatgcgc tcgtctcgct
      661 cgccgggctc gcggtcgatc agcagaattt cgaacgagcg ctcgagctgt tgcgcgaggt
      721 cgtgagcagc aacccggccc ggacggagct gcaacgcaac gtcgacgtat tggagaactt
      781 ggttctggag cggacgaggg actgacgcgg aatgaagcgc tgggcgcaac tgtggctctc
      841 gatcatgctg gcaccggccg cgcacgccgc cgagatcgag ctcacgatca caccgcccga
      901 gcccgagtgg ctgctttacc ccgagctctc gccgggacct tgcgctttcc cgggcggttt
      961 tcggctcgaa gcatgcccga agcaactcat gccggagggc atcgccaaca tcggcgagca
     1021 atcgctcgtc atcgacctcg ggccgctgct cgccgccggc aactacgagg ccgcgttggc
     1081 gcgcttgcgc ctgaacttcg gtccccatct cctgctgctc gaagcgggcg acgtcgacgg
     1141 ctttcgccga gcgcgccttc cgacgtcggg cgaaggaact ttgcggccgg gaccgggctc
     1201 ccggggacga gacacgccgt ccatgaactt caccatggag cgcagcaacg tgccgacggc
     1261 cgaaggggcc ggcgacacgc ccgggcggct gacgggccgg agcagccgct caggcgttgc
     1321 tccggacagc atcagcgcga gcatgctcta cgtcatcggc cactgctact tttcgctgca
     1381 gcggtatctg cccgcggaga ccgcgttcaa gctggcgctg gttgcgcttc cgaatcacgt
     1441 ccgcgcgcac gagtcgctcg cgattctcta ccttcgaacc gggcgctatg cggatgcgcg
     1501 cgtgcacctc accaagctcg ccgagctcgg ccgcaacacg gcgcaggttc actcggcgcg
     1561 cggctacctc gagcagaaga cgcaccgcca ttggtacgcc gcggatgcgt ttcaaaaagc
     1621 gctcgtgctc gcgcccgacg atcgcggcgc ccagcgaggg ctgcttcttg cgctgaccga
     1681 aacgcacgag tatgcgaaag ccaaggcgct cgtcgatcgg ttgttgcgcg acgagcccga
     1741 cgatcgggat ctctggctgt accgcgcgca gatcgcgctc tccaccgacc aacgcgccgt
     1801 ggcgctcgcg agcctcgaga cggcgctcgc gctcggcgag gattccgtcg agaaccggcg
     1861 tgcctgcttc gaattgcaga tggagaacgg caacatcgcg aggggcgtcg agctgcttcg
     1921 aggcttgcgc gcacgcgacc tgccctttcc gctcgtcgat caagcgctcg ggtggctcgc
     1981 gaacgaaagc cagtgggacc gcttccgcga ggtcttgggc tccgtcgacc gcgaaaggct
     2041 cgacggcgtc gagcaaagcc ggctgctgac gcagcgcgcc gcgcttgccg cgcacgacgg
     2101 caatcggcgc gccgcgagca cggcgttgca ggaagcgctg gcgctcgatc cgtcgaacgc
     2161 cgacgcgctg atcgcgctcg ggcaaatcta ccgcgtggat cgcgactatg ggcgcgccga
     2221 cctcttgttc cagcgcgcga gcgcttacgc ggccgctcgc gagagcgcgc tcgtcgcgcg
     2281 tgccgacgtc gccagcgacg agggcaactt cgacggcgcg ctcgcggccc tgcgcgacgc
     2341 ggccgccctc gaccccacac gcggcgacct gaaacgcaac atcgaagtct tggagaacct
     2401 gtcgctgctg cgcacgcagc gttgagcctc gcacacgtca atgccgcgtc ggccgcgatg
     2461 agggcgggca cgcgatttgc agtgtctcag ggcgtaaaac atagggagat gtcgcccatg
     2521 cgcccgaata accactacat tctgccgctg ctcgcggctc tcgcggcttc cggcgttgct
     2581 tcggcgcagc gcgagcgaac cgagcccccc gttgcggagg tcgagcccgc gccgaacccc
     2641 tcgacgagcg agcgagtcgg ccccagcgac ccgggcctcg cgctcgaagt gcaggcgcgc
     2701 ctctatcaag cgctcacgga cagcaacatg agcgtgctcg tgcgctacgg cgtcgcaacg
     2761 ctcgacggcg tcgtgcgcac ggaggcggac cgccagcgcg cggaggaaat cgcgctccaa
     2821 gtccccggca tcgacagcgt cgtcaacgac ctcaccgtcg ctccgcccat cgcgattgcc
     2881 gcagtcgacg aggctcaagc catcacggcg caagagaaca ccaaggtcga ggatcaggtg
     2941 gcgcagcagc tgcgcgccga tgcaacgctc gggtcgcgcg acatccgcgt cgttgccgac
     3001 cggctcacga acacgatcac gctgagcggc acggtcagca ccgaggaaga gaaggagcgc
     3061 gcgggccaga tcgccgtgaa ggcgttccct gtcgggcaag tgcgcaacca gctcgaagtg
     3121 cgacaacggc tgtagcgcac cggcccgaac gggacggtta cagcgacttt aggtactcga
     3181 ccagctcgcg cttctcgccg ttgctcaagc cgagcccctg gaacgagtcg tagtgatcga
     3241 cgacctcgcg caacgtcgcg aacctgccgt cgtgatagaa gccgcccttc gtgtgggtcc
     3301 agagcccgcg tagcggcgac gtgcgataac cgtcggtcgg gctgcgctgc gcctggaacg
     3361 cgtcgatgcc gatatcctcg ggcttgtgta ggttgtggcc gggctcggta aagatcggcg
     3421 ggacgtgaca cgtcgcgcag tgcgccttgc cgtcggccgc gaacagcgct ttgccccgct
     3481 ccgccgcgcg cgcgtcgaag cttcctttcg gcggcggcgg cgcggcgatc gcgagctgat
     3541 agagctgcag cgcgccgagc ttcggcgtca cgaggtcggg ctcgcggcgc gtgttgccga
     3601 agtcgttcgc cgccgcgatc ggaaacttgc tcgcgtcctc gaggcgcgga tcgtacagcg
     3661 tgcctttgcc ctgcatctcg agcaccgcga cgaacgcgtt ccagtgcgtg acggagcccc
     3721 aacccgtcca ggtatgaaga ttcacaccgg caagaccgaa ggccggcggc agcagcgtcg
     3781 cggcgctctt gccgtcgggc cgaaacgcct tgccgtcgag cagcagctcc gcgtcgaact
     3841 tgccggggcc ccagctcctc aagaccttgc gcacggtcgc ctcgtcgacg ccgaggagct
     3901 gcgcgaacgg cgccacgctc ggcgcgaacg cgatgatctc gccgacattc aagtcgcggt
     3961 tcgcccagcc gtcgagccgc gcgccgatgc cgggcgcgaa cgagttgtcg accgtgctat
     4021 ggcagagcgc gcacgtgatg ccgaccgatt cgacgttgcc cgcgcggtta gtgaacgcgc
     4081 gcacgccgac gacggcattg cgcttcaaga gcgcgagcgt gacggccgga tcgtcgagat
     4141 tcactttgcc tgcccgcagc gcctgcacga gcggcggcgg caacgcgcgc acgtcgacct
     4201 tgagcccgag ctcgagcgcc gccttcgggc tcaggcccgg gcccaccccg ccgttcgcgg
     4261 cgcccgcgac cgactcgtgc aagtgcagcg cgccgcccca gaagtcctcg tccccgaacg
     4321 tgtcgaaacg aaacgtgtta cggccgcgcg cgatctgcgt ggccgcgtgc tgccagacgt
     4381 cgcccccggg cggctgcggc cggtcgtcgg cgcgcgcgcc tccgactgcc atcgtgaccg
     4441 cgagcgccgc gaacgtgctg aagccgcgag atagagtcga catggccgtt gctccttcct
     4501 tggaaatcac tgacatctga ttcgtgcgag gaccgttccg acgatcgcgt cgcagtgctg
     4561 cccgccgaac gggaacgcag tcggaacgac gtcgtcgagc gtgcgctgca gctgctcctg
     4621 caacatccga cgcgcgcggt gaaatcgcgt cttgaccgtc gcggggttga tctcgagcag
     4681 ctcggccgtc tccgcgatgc tgagctgctc gacgccgcgc agcatgaaaa cccgccggaa
     4741 ctcgtccggc agcgtgtcga tggccgcctg caaaatccgc agcaggtcgc ggctcgacgc
     4801 cgcgttctcg ggctcggctt ccggaagatc cgtcagcgcg agcgcatgct gctcggcatc
     4861 gacgatcggc ggcgcacggc gcacgcggcc gagcgcctcg ttgacggcga tacgcgcgag
     4921 ccaggccttg aagccggcgg gcccgcggaa ctggccgagg tggtaataag cgcgaacata
     4981 ggccgcttgc acgacgtcgc gcgcctcgtc gtcgtcacgc agaatgccgc gcgcgaggcg
     5041 aaacagcaga cggttgtagc gccgcatcac gagctcgaag agcgccgaat cgcccgtgag
     5101 cacgcgcgca acgatcgcct cgtcggcgag cacggcgtcg gggatcgcgg ccgccgacag
     5161 ccccggctcg ggaatcggcg gctcgagagt caccggcccg gcgagccgcg catcgacgag
     5221 cgccgcgtca gggatagccg catcgacgac ggtgttgggc gccaggccgt cctgcgcgcc
     5281 gagctctggg cgctcgttca tgcgtacatg atgcgcccac gggaaaaaag gttcccacgg
     5341 ccaagatggc cggggccgtg ggcgaaaacg tttggagtgg gtctactgcg ggcagcgcag
     5401 gcgacgcgct aatcagcgtc ggctgcgtcg gtcgagcacg tcattctgcg cgaatgctcg
     5461 aggcagttcg cacattgcac cgaaccgtcc gaatagagat ggaacgtcag cgatccgcac
     5521 gagcaggccc agattccgag ccgagactgc accgcgacgc gggcgcgcgg cggaaacggt
     5581 atgacgttgc tgttcgaggg gtccgacatt ccatgtctag tagagcaaaa cgccgcgagg
     5641 ccggccgtga cgcaggtaac gctcggctga aacagtcgca cgtccgactg gtctgctgag
     5701 aggccgtcga tcgctttcgc cgcgcgcgtc ggcgtcatcg cgttcgcgac tcgccggccg
     5761 tgccgagcgt gacgggcacc tcgatgcgcc gatcgttgcg aatgacggtc agcgtcacgc
     5821 ggtcgccgaa ctcgtgctcg tcgaggacgc cgatgagatc ggcttcggtc tcgacgggcc
     5881 ggccgtcgac ggccgcgatg atgtcgccga tgatgatgtt gccgtttcgt tcttggcgcg
     5941 tcgggcgcag gccaacccgc tccgccggcg agccgccggc gacgccgagc acgagcacgc
     6001 cgcccgcgcc gtctctaccg aacagttgct gcgtcacgcg gtcgtccgag tcgaccccga
     6061 gcgtcggccg cacatagcgg ccatagccga tgatccgcgg cacgacgcgg t
//
LOCUS       DASSNN010000334         4385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1192417, whole genome shotgun sequence.
ACCESSION   DASSNN010000334 DASSNN010000000
VERSION     DASSNN010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192417"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1767)
                     /locus_tag="VFL84_06095"
     CDS             complement(<1..1767)
                     /locus_tag="VFL84_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7608229.1"
                     /translation="MDVTDIIDPLNDAQREAVTAPDRPTLVLAGAGSGKTRVLVHRIA
                     WVIRVGGVAAQHVLAVTFTNKAAAEMRDRVEALLGVPTRNLWIGTFHGLSHRLLRIHW
                     REAGLSEGFQIMDSEDQLRMVRRMLRSLELDENVWVPKEIQWFINAQKDEGLRPKDLR
                     DEGNAGRRQMIELYERYEQACAAASVIDFGELLLRSLELLRDNLELQREYQHRFRHVL
                     VDEFQDTNAIQYRWLRLLTGPAGAPFVVGDDDQSIYRWRGARVENLYRFQQDYAGTQV
                     VRLEQNYRSTATILNAANAVIANNAGRLGKTLWTEGPTGEPIKLYAAFNERDEAEFVI
                     NRIQDHVDKGGKRSEAAVLYRSNAQSRVFEEGLLAAGIPYRVYGGLRFFERAEIKDAL
                     AYLRLVMNRDDDPSFERVVNVPPRGIGARTVEAVREHARGAGTSLWRAAAALIAEGTL
                     AKRAAESLFGFLNLVEKLDADTRGDALHEQVARVIERSGLIAHHGKNKASDLSGEARV
                     ENLDELVNAAREVMRVEDGDLPPLAEFLAFAVLESGDGQAEAYQDSVQLMTLHSAKGL
                     EFSLLFLCGMEDGLFPHQRSLND"
     gene            complement(1811..2725)
                     /gene="cyoE"
                     /locus_tag="VFL84_06100"
     CDS             complement(1811..2725)
                     /gene="cyoE"
                     /locus_tag="VFL84_06100"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET7608230.1"
                     /translation="MLTKELGKLDESAGAHWRDDSALTKPGVVQLLVFTAIVGMFLAV
                     PGMVPWSALLAGSLGVGLAAAAGAVVNQILDQRIDAQMARTRNRPLPGGRIGERDALA
                     FAMGLGLAGLAVLVLFVNVLTAALTFASLIGYAVVYTVYLKRATPQNIVIGGAAGAAP
                     PVLGWTAVTGTVDPHALLLFLIIFTWTPPHFWALAIARRSDYERAEIPMLPVTHGVDV
                     TKSFIVYYTILLVLVTILPYITGMTGPLYLLGALLLGAGFLFYALQLKWNPREDSPMA
                     TFRYSISYLMALFSFLLIDHYMPAVWPA"
     gene            complement(2703..3671)
                     /locus_tag="VFL84_06105"
     CDS             complement(2703..3671)
                     /locus_tag="VFL84_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET7608231.1"
                     /translation="MNVWFVRLARIGAVLALAVVMLGAYVRLSDAGLGCPDWPGCYGR
                     LVVPHPATSAEELGLEYTRPLEHGKAWREMIHRYLASTLGLVCVALAGLAWRNRRDPA
                     QPKIMPAALVALVIFQGLLGMWTVTLLLKPIVVVAHLLGGLATLTLLASLGRWRPARF
                     PSPTPALRALGLGAAAALVLQISLGGWTSANYAALACPDFPTCQTQWWPAVADFKEAF
                     VLWRGIGIDYEGGVLDHPARVAVHFTHRLGALLAAVLIALLGFRLAREPATRADGAAV
                     LAVLALQLSLGVSIVWFGVPLAVAVLHNGVAALLLLAVINANQRIR"
     gene            complement(3671..4204)
                     /locus_tag="VFL84_06110"
     CDS             complement(3671..4204)
                     /locus_tag="VFL84_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608232.1"
                     /translation="MKHKTLTPWLVALVCVGPFAAALFIYYGPLGRAWLPQLPGEREL
                     LEKPVALPAAWAEGGEAPWQLIYARMSPCEQQCAQHLGRLLQVQLALGRDQDRVQRVF
                     WHAGALPALDDPKLMMHALDDDIGRGVAEALGAERLEDGRVYVADPRGFVIVSYPPDV
                     EQKELLRDLERLLTGPE"
     gene            complement(4201..>4385)
                     /locus_tag="VFL84_06115"
     CDS             complement(4201..>4385)
                     /locus_tag="VFL84_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HET7608233.1"
                     /translation="VFDYQLLLDPGSDDGYVRDWRAPALSPERHLTYAGQWLALGLGA
                     LAAAVVMAVKAMRRGR"
BASE COUNT          771 a         1492 c         1468 g          654 t
ORIGIN      
        1 gtcgttcagc gagcgctggt gcggaaagag gccatcctcc atgccgcaga ggaagagcag
       61 agagaactcg aggcctttcg cggagtgcaa cgtcatgagc tggacgctgt cctgatacgc
      121 ctcggcctgg ccgtcgccgg actcgagcac ggcgaacgcg aggaactcgg cgagcggcgg
      181 caaatcgccg tcctcgacgc gcatgacctc gcgcgcggca ttgacgagct cgtcgaggtt
      241 ctcgacgcgc gcttcgccac tcaagtccga cgccttgttc ttgccgtggt gcgcaatgag
      301 gccgctacgc tcgatgacgc gcgcaacttg ctcgtgcagg gcgtcgccgc gcgtgtccgc
      361 gtcgagcttt tcgacgaggt tcaggaagcc gaacagcgac tcggcggcgc gcttcgcgag
      421 cgtgccttcg gctatcagcg ccgccgcggc gcgccagagg ctcgtgccgg cgccgcgcgc
      481 gtgctcgcgc acggcctcga ccgtgcgcgc accgattccg cgcggcggaa cgttgacgac
      541 gcgctcgaac gacggatcgt cgtcgcggtt catgacgagc cgaaggtacg cgagcgcatc
      601 cttgatctcg gcgcgctcga agaagcgtaa gcccccgtag acgcgatacg ggatgccggc
      661 cgcgagcagc ccttcctcga acactcgcga ttgagcgttc gagcggtaca gcaccgccgc
      721 ttcgctgcgc ttgccgcctt tgtcgacgtg gtcttggatg cggttgatga cgaactcggc
      781 ctcgtcgcgc tcgttgaacg ccgcgtagag cttgatcggc tcgcccgtcg ggccttcggt
      841 ccatagcgtc ttgccgagcc tccccgcgtt gttggcgatg acggcgttcg cggcgttcaa
      901 gatcgtggcc gtggaacgat agttctgctc gagccgcacg acctgcgtgc cggcatagtc
      961 ctgctgaaag cgatagagat tctcgacgcg cgcgccgcgc cagcggtaga tcgactggtc
     1021 gtcgtcgccg acgacgaacg gcgcgcctgc cgggccggtc agcaagcgca gccagcggta
     1081 ctggatcgcg ttcgtgtcct ggaactcgtc gacgagcacg tgccggaagc gatgctggta
     1141 ctcgcgttgc agctcgagat tgtcgcgcag cagctcgagg ctgcgcagca gcagctcgcc
     1201 gaagtcgatg accgaggcag cggcgcacgc ctgctcgtag cgctcgtaga gctcgatcat
     1261 ctgccggcgg ccggcgttac cctcgtcgcg caagtccttc ggccggagtc cctcgtcctt
     1321 ctgggcgttg atgaaccact ggatctcctt cggtacccag acgttctcgt cgagctcgag
     1381 cgaacgcagc atgcggcgga ccatgcgcag ctggtcctcg gaatccatga tctggaaccc
     1441 ttcgctcaaa cccgcttcgc gccaatggat tcgcaagagt cgatgcgaca ggccgtggaa
     1501 cgtgccgatc cacaagtttc gcgtgggcac gccgagcagc gcctcgacgc ggtcgcgcat
     1561 ctcggccgcg gccttgttcg tgaacgtcac ggcgagcacg tgctgcgcgg cgacgccgcc
     1621 gacgcggatg acccatgcga tccgatggac gagcacgcgg gtcttgccgc tgcccgcgcc
     1681 ggcgagcacg agcgtcgggc ggtcgggcgc cgtgacggcc tcgcgttgcg cgtcgttcaa
     1741 agggtcgatg atgtcggtaa cgtccatcta gtcgcgactg gcgcgcttac tgcggaacgg
     1801 cgggatcagc tcacgccggc cagacggccg gcatgtagtg atcgatcagc aggaagctga
     1861 aaagtgccat cagatacgag atcgaatagc ggaacgtcgc catcggcgag tcttcgcgcg
     1921 gattccactt cagttgcagc gcatagaaga ggaagcccgc gccgagcagc agcgcgccga
     1981 gcaagtagag cggccccgtc atgcccgtga tgtacggcag gatcgtcacg agcacgagca
     2041 gaatcgtgta atagacgatg aacgacttcg tcacgtcgac gccgtgcgtg accggcaaca
     2101 tcggaatctc tgcgcgctcg tagtccgagc ggcgcgcgat cgcgagcgcc cagaagtgcg
     2161 gcggcgtcca cgtgaagatg atcaagaaca agagcagcgc gtgggggtcg accgtgcccg
     2221 tcacggccgt ccaaccgagc accggcggcg cggcgccggc ggcgccgccg atcacgatgt
     2281 tttgcggcgt cgcgcgcttc agatagaccg tgtagacgac cgcgtagccg atcaaagacg
     2341 cgaacgtcag cgcggccgtc agcacgttga cgaacagaac gagcacggcg aggcccgcaa
     2401 gccccaatcc catggcgaac gcgagcgcgt cgcgctcgcc gatgcgccca ccgggcagcg
     2461 gccggttacg cgtgcgcgcc atttgcgcgt cgatgcgctg atcgagaatc tgattgacga
     2521 cggcgcccgc cgcggcggcg agcccgacgc ctaagcttcc cgcgagcaac gcgctccacg
     2581 gcaccatgcc gggcacggcg agaaacattc cgacgatggc cgtgaagacg agcagctgca
     2641 cgacgcccgg cttcgtgagc gcggagtcgt cgcgccagtg cgcgccggcg ctctcgtcaa
     2701 gcttacctaa ttctttggtt agcattgatc acggccaaca ggagcaacgc ggcgaccccg
     2761 ttgtgcaaga ccgccactgc caacgggacg ccgaaccaga cgatactaac accgaggcta
     2821 agctgcagag ccagcacggc caggaccgcg gcgccgtcgg cccgcgtggc cggctcgcgc
     2881 gcgagacgaa atccaagcag cgcgatcagc acggccgcga gcagcgcgcc caagcgatgc
     2941 gtgaagtgga cggctacgcg cgcgggatgg tcgagcaccc cgccttcgta gtcgatgccg
     3001 atgcctcgcc agagcacgaa cgcttccttg aaatccgcga cggcgggcca ccattgcgtt
     3061 tggcacgtgg ggaaatcggg gcacgcgagc gcggcgtagt tcgcgctcgt ccaaccgccg
     3121 agcgagatct ggagcacgag cgccgccgcc gcgccgaggc cgagcgcgcg cagcgccggc
     3181 gtcggcgaag gaaatcgagc cgggcgccag cggccgagcg acgcgagcag cgtgagcgtc
     3241 gcgaggccgc cgagcaagtg cgcgacgacg acgatcggct tcaacagcag cgtcacggtc
     3301 cacatgccga gcaaaccctg gaagatcacg agcgctacga gcgccgcggg catgatcttc
     3361 ggctgcgccg gatcgcgccg attgcgccac gccagacctg cgagcgcgac gcagacgagg
     3421 ccgagcgtgc tcgcgagata ccggtggatc atctcgcgcc acgctttgcc gtgctcgagc
     3481 ggccgcgtgt actccagccc aagctcttcg gccgacgtcg ccggatgcgg cacgacgagc
     3541 cggccgtaac agccgggcca gtccgggcag ccgagccccg cgtcgctcaa acgcacgtac
     3601 gcgccgagca tgacgacggc gagcgccagt acggcgccga tgcgcgccaa gcgtacaaac
     3661 cacacgttca tcattcgggt ccggtcagca atctctcgag gtcgcgcagt agctcttttt
     3721 gctcgacgtc cggcggatag ctcacgatca cgaagccgcg cggatcggcc acgtacacgc
     3781 ggccatcctc gagccgctcc gcgccgagcg cctccgcgac acctcggccg atgtcgtcgt
     3841 cgagcgcgtg catcatgagc ttcggatcgt cgagggcggg cagcgcgccg gcgtgccaga
     3901 aaaccctttg cacgcgatct tggtcgcgtc cgagcgccag ctgcacctgc aagagccttc
     3961 ccaagtgctg cgcgcattgc tgctcgcagg ggctcattct tgcatagatc aattgccacg
     4021 gcgcctcgcc gccttcggcc caagccgccg gcagcgcgac gggcttctcg agcagctcgc
     4081 gctcgcccgg aagctgcggc agccaggcgc ggccgagcgg cccgtaataa atgaatagcg
     4141 ccgccgcgaa cgggcccacg cagacgagcg cgaccagcca gggcgtcagc gttttatgtt
     4201 tcaccgcccg cgcctcatcg ccttgaccgc catcacgacc gcggccgcga gcgctccgag
     4261 cccgagcgcc agccactggc cggcgtatgt cagatggcgc tccgggctca aagccggcgc
     4321 gcgccagtcg cgaacgtagc cgtcgtccga accggggtcg agcagcagct gatagtcgaa
     4381 gaccc
//
LOCUS       DASSNN010000335         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1300219, whole genome shotgun sequence.
ACCESSION   DASSNN010000335 DASSNN010000000
VERSION     DASSNN010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300219"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VFL84_06120"
     CDS             complement(<1..125)
                     /locus_tag="VFL84_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HET7608234.1"
                     /translation="MRNIGLTVLLLSLSNIFMTFAWYAHLKELSNRPWIVAALVS"
     gene            204..1661
                     /locus_tag="VFL84_06125"
     CDS             204..1661
                     /locus_tag="VFL84_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET7608235.1"
                     /translation="MAVVSERPKAKSLAPLRALLPFLAPHRRLLVLAVAALLVAAAAQ
                     LALPVALRSLIDEGLALRDAATINRYFVLFLVAGAVFGAFAALRFFFIMRLGERVVAD
                     IRNAVYAKVIRMDPTFFEVTRTGEVLSRLTADTTLIQAVAGFGISLTLRSALTLIGSL
                     VLLVLTSPTLTLLIVVMIPLVVGPLLLLGRRVRKLSRDSQDRIADASALAGETINAIQ
                     TVQAFNLEELQSRRFHDAVEHSFAVAVRRIRVRALLTALATMLVFGGITVVLWIGAHR
                     VLAQSMTFGELSQFLLYAGYMGIAAASLSEMWGEVQRGAGAMERLAELLAAEPTVKTA
                     AQPARLPDVSRGRIELEHVSFRYPSRPETAALDDFTVRVEPGETVAFVGPSGAGKSTT
                     FQLLLRFYDPAAGRVLVDGVDIARADPEEVRSRIGLVPQDTMLFGASARENIRYGRPN
                     ASDADIEAAARAAEADEFILVDAGAHLGGSPTKAR"
     gene            complement(1658..>2150)
                     /locus_tag="VFL84_06130"
     CDS             complement(1658..>2150)
                     /locus_tag="VFL84_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7608236.1"
                     /translation="LFDDTIAANIAYGAMAGADRGAIEQAAAAAHALDFVRALPQGFD
                     TPVGEHGVRLSGGQRQRIAIARAILKDPPILLLDEATSSLDAASELLVQQALEKLMAQ
                     RTTIIIAHRLATVLKADRIVVLDHGRIIATGTHAELLQTNELYARLAALQFADLSAVG
                     NAA"
BASE COUNT          301 a          741 c          734 g          374 t
ORIGIN      
        1 cagctcacga gcgcggcgac gatccacggt cgattgctga gctccttcaa gtgcgcgtac
       61 cacgcgaacg tcatgaagat gttgctcaac gaaaggagca ggacggtcag gccgatattg
      121 cgcatgagcg ttgccggggt gccgcgacgc cgcgcagtat gccacggcgc ctgagaggcc
      181 cgcgttcgcg ctaagcttgc gcgatggctg tcgtttccga acgtcccaag gccaagtcgc
      241 tggcgccgct gcgcgcgctg ctgccgttcc tcgcgccgca ccggcgcttg ctcgtcctgg
      301 ccgtggcggc gctgctcgtg gccgcggctg cgcagctcgc gttgcccgtc gcgttgcgtt
      361 ccctgatcga tgaagggctc gcgcttcgcg acgccgcgac gatcaaccgc tatttcgtcc
      421 tgttcctcgt cgccggtgcc gtcttcggcg cgttcgcggc gctgcggttc ttcttcatca
      481 tgcggctcgg cgagcgcgtc gtcgcggaca ttcgcaacgc ggtctacgcg aaagtcatcc
      541 gcatggaccc gaccttcttc gaggtcacgc gcacgggcga ggtgctctcg cggctgaccg
      601 cggacacgac gctgattcag gccgtggccg gcttcggcat ctcgctgacg ctgcgttccg
      661 cgctgaccct gatcggctca ctggtgctgc tcgtcttgac gagcccgacg ctgacgctgc
      721 tgatcgtcgt gatgataccg ctcgtcgtgg ggccgctgtt gctgctcggc cgccgcgtgc
      781 gcaagctctc gcgcgattcg caggatcgca tcgccgacgc gagcgcgctt gccggcgaga
      841 cgatcaatgc catccagacc gtgcaggcgt tcaacctcga agaattgcag agccgccgct
      901 tccacgacgc cgtcgagcat tcgttcgcgg tcgccgtgcg ccgcatccgc gtgcgtgccc
      961 tgctcacggc gcttgcgacg atgctagttt tcggcggcat caccgtcgtg ctgtggatcg
     1021 gcgcgcatcg agtcctggcg cagtcgatga cgttcggcga gctgagccag ttcctgctgt
     1081 atgcgggcta catgggcatc gccgctgcat ccttaagcga gatgtggggc gaagtgcagc
     1141 gcggagccgg cgcgatggag cggctcgccg agctgcttgc ggccgagccg acggtcaaga
     1201 ccgcggcgca gccggccagg ttgccggacg tgagccgcgg ccgcatcgag ctcgagcacg
     1261 tgagcttccg ctatccgtcg cgtccggaga cagccgcgct cgacgatttc accgtgcgcg
     1321 tcgagccggg ggaaaccgtt gcattcgtcg gcccgtccgg cgccggcaag agcacgacgt
     1381 ttcagctctt gctgcggttc tacgatcccg ccgcaggccg agtgctcgtc gacggcgtcg
     1441 acatcgcgcg cgcggatccc gaggaggttc gaagccgcat cggtctggtg ccgcaggaca
     1501 cgatgctgtt cggcgcctcg gcgcgcgaga acattcgcta cggccggccc aatgccagcg
     1561 acgccgacat cgaggccgcc gcgcgcgcgg ccgaagccga cgagttcatc ctcgtcgacg
     1621 ccggcgcgca cctgggcggc tcgccgacga aggcacgcta ggcagcgtta ccgaccgccg
     1681 acaagtctgc gaactgcagc gctgccagtc gcgcgtacag ctcgttcgtc tgcagcagct
     1741 cggcatgtgt gcccgtcgca atgatgcggc catggtccag caccacgatg cgatcggcct
     1801 tcagcacggt cgcgaggcgg tgcgcgatga tgatcgtcgt acgctgtgcc atgagcttct
     1861 cgagagcctg ctgcacgagc aactcgctgg ccgcatcgag cgagctcgtc gcctcgtcga
     1921 gcagcaggat cggcggatcc ttgaggatcg cgcgcgcgat cgcgatgcgc tggcgctggc
     1981 cgcccgacag gcgcacgccg tgctcgccga ccggcgtgtc gaagccctgc ggcagcgcgc
     2041 gcacgaagtc gagcgcgtgc gcggcggccg cggcctgctc gatcgcgccg cgatccgcgc
     2101 cggccatcgc gccgtaggcg atgttcgccg cgatcgtgtc gtcgaacagc
//
LOCUS       DASSNN010000336         5717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1574449, whole genome shotgun sequence.
ACCESSION   DASSNN010000336 DASSNN010000000
VERSION     DASSNN010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574449"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFL84_06135"
     CDS             complement(<1..191)
                     /locus_tag="VFL84_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7608237.1"
                     /translation="MDGETAIRATALTKRFGQVVAVDHLDLTVRRREVYGFLGPNGSG
                     KSTTIRMLCGLLNPTSGDI"
     gene            complement(191..1156)
                     /locus_tag="VFL84_06140"
     CDS             complement(191..1156)
                     /locus_tag="VFL84_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET7608238.1"
                     /translation="MRAEARLMLLALLGSTALAGCEQRGPAPILGTLEWDRVAVTAEL
                     AEPVLRWHAAEGDRVEAGAPLLELDARRQDARIAEAESELKMAEARLAELAHGARIET
                     IDAARANLSRARAAQEDAETEYMRVAELRKRELVAQSVVDQALAARNQRRAETSQAEA
                     QLRELTQGTRPEQIEQASAAVAAARAALETLKLTRERLTVRAPRAGRVDALPFHPGDQ
                     PPVGAELVSMLVGDAPYARVFVPAPRRAEVDAGTRFEIHVEGIAMPFTAKLRSIRSEP
                     SFTPYYALAGDDASGLVYRAELLLDVGNGAAELPAGLPVTAVLVQ"
     gene            complement(1146..1817)
                     /locus_tag="VFL84_06145"
     CDS             complement(1146..1817)
                     /locus_tag="VFL84_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7608239.1"
                     /translation="MSAKLTRRRKPEPRRPGRPRADEANQRERLLDAAVDCFAGDGIA
                     ATSLRAIALKAGVTPALVNYYFGSKAALVDAFIAERLTPVAASLREGLVAAGPDSRAL
                     MSAFVRGMHDVVARHPWWPALWVREVLNEKGALRDVLRKQIAPQVAQLLAGALADAQK
                     RGELAADLDPRLTVVSLMGLTMMPLAAEHIWRRVFAANDIDRDLLLRHTLALLDHGLG
                     GKHAR"
     gene            1940..2605
                     /locus_tag="VFL84_06150"
     CDS             1940..2605
                     /locus_tag="VFL84_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608240.1"
                     /translation="MLRASIKRRAFLAAAAGMAGLTAFRAGRTAAQSAPVRVTREEPI
                     ISRTQFDPARPPPDMPELTPPEAGVCRTTFELSAGVTYSAERLSRTAARISVDGLDIV
                     TRVRFEIFTARGAPPKLAAHEEGHRSIGEHYYKDAAQIAEVIGKRLIGTTFDGSGADQ
                     EAAQRNAFEKVIAEIERDYMARMRTPSAAANERFDEITSHGLDAIDEADAVAVAIAAV
                     GAR"
     gene            complement(2637..2828)
                     /locus_tag="VFL84_06155"
     CDS             complement(2637..2828)
                     /locus_tag="VFL84_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608241.1"
                     /translation="MTMGPGTKDPWLRWAVFVVPAFATALCWRLTGGLPFVNRVAIGV
                     AVALVVFGVVHVLVKGWPR"
     gene            complement(2878..3114)
                     /locus_tag="VFL84_06160"
     CDS             complement(2878..3114)
                     /locus_tag="VFL84_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608242.1"
                     /translation="MNAACAGTLRELTAEETALVGGGEFTWGGFFGHVAGGALAGGLA
                     GAAAAGVGAGPGALAGGLLGGIEYSVSAFVEDYF"
     gene            complement(3224..5374)
                     /locus_tag="VFL84_06165"
     CDS             complement(3224..5374)
                     /locus_tag="VFL84_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HET7608243.1"
                     /translation="MSLAERLRLSLRRKLPVLLQTEAAECGVACLAMVASYHGHAVDV
                     ATLRRRFTVSMRGATLAALMQLAARLGLETRAVRVELEDLARLRLPCILHWSFTHFVV
                     LRRVTRRGLLVHDPARGFRRVRRAEAGSSFTGVALELWPSPRFTPRAPAPAVRLRALV
                     GNVRGLAGALAQILLLAATLETFVLANPLLMQWVVDHALVTASRDLLTLLALGFGLLV
                     LFEQSTAALRGVVIMHFEATLNVQWQANVLAHLLRLPLPYFEKRHLGDIVSRFRSIDV
                     IQRTVTTTFVEAIVDGAMSLLLLAVMLCYSAPLAAVCVAAIAVYAALRWVAHAPLRDA
                     RHEQIAAAAKQDSHFIETARGVRAIKLFARGDERRGAWLGLLVEQINAGARVQRLGLA
                     LRAGNGLTLGLENVLVVYLGASLVLSGALSTGMLLAFVAYQRQFAVRVSTLIDKLAEV
                     RLLRLHCERLGDIVLTAPEQHDGSGRLLVSAATPGLAIRARGLRFRYGEHEPYVLDGV
                     DLEIAPGECVALVGASGSGKSTLLSLLLGLLAPSAGEIAIAGVAPRELAGAQRTVAGV
                     MQGDCLFAGSIADNISFFAADADQRRVEECARLAAIHSDIAKLPMAYNTLVGFMGSTL
                     SAGQQQRILLARALYAQPAVLILDEATSHLDAPREAAIAAAVAQLGMTRIIAAHRRET
                     LAAADRIVRLDAGRIVAEAPGPRRTVSLPAGESR"
     gene            5487..>5717
                     /locus_tag="VFL84_06170"
     CDS             5487..>5717
                     /locus_tag="VFL84_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608244.1"
                     /translation="MPAASIERMESDNSAPLPLFRPAAIAAQRRDESGGIVLTRAWSF
                     TILSAFLAALAGALISFAVFGSYTAHTTLRGRL"
BASE COUNT          894 a         2058 c         2016 g          749 t
ORIGIN      
        1 tcgatgtcgc ccgaggtcgg attcagcagg ccgcagagca tacggattgt cgtcgacttg
       61 ccggagccgt tcggccccag gaagccgtag acctcgcgcc ggcgaaccgt gaggtcgaga
      121 tgatcgacgg cgacgacctg cccgaagcgc ttggttagcg ccgtcgcgcg gatggcagtc
      181 tcgccgtcca tcactgcacc agcacggccg tcacgggcag gcccgccggc agctcggccg
      241 cgccgttgcc gacgtcgagc agcagctctg cgcgatagac gagcccgctc gcatcgtcgc
      301 cggcgagcgc gtagtacggc gtgaagctcg gctcgctgcg gatgctgcgc agcttcgccg
      361 tgaacggcat cgcgatgccc tcgacgtgga tttcgaatcg cgtgccggcg tcgacctccg
      421 cgcgccgcgg cgcgggcacg aacacgcgcg cgtacggcgc gtcgccgacg agcatgctga
      481 cgagctccgc gccgacgggc ggctggtcgc cggggtggaa cggcagcgcg tcgacgcggc
      541 ccgcgcgcgg cgcgcgcacg gtgagccgct cgcgtgtcag cttcagcgtc tcgagcgccg
      601 cgcgcgcggc ggcgacggcg gccgacgctt gctcgatttg ctcgggccgc gtgccctgcg
      661 tgagctcgcg cagctgcgcc tcggcttgcg aggtttcggc gcgccgctgg ttgcgcgccg
      721 cgagcgcctg gtcgacgacc gattgcgcga cgagctcgcg cttgcgcagc tccgcgacgc
      781 gcatgtactc ggtttcggcg tcttcctgcg cggcgcgcgc acgggacaga ttcgcgcgcg
      841 cggcgtcgat cgtctcgatg cgcgcgccgt gcgcgagctc ggcgaggcgc gcctcggcca
      901 tcttgagctc gctctcggcc tcggcgatgc gcgcgtcctg gcggcgcgcg tcgagctcga
      961 gcagcggagc cccggcctcg acgcgatcgc cttcggcggc gtgccagcgc aggacgggct
     1021 cggctagctc ggcggtcacg gcgacgcggt cccactcgag cgtgcccagg atcggcgcgg
     1081 ggccgcgctg ctcgcagccc gcgagcgccg tggagccgag cagggcgagc agcatgagac
     1141 gggcttcagc gcgcatgctt gcctccgagg ccgtggtcga gcagcgccag cgtgtggcgc
     1201 agcagcaagt cgcggtcgat gtcgttggcc gcaaagacgc ggcgccagat gtgctcggcc
     1261 gcgagcggca tcatcgtgag tcccatgagg gatacgaccg tgagccgggg gtcgaggtcc
     1321 gcggcgagct cgccgcgctt ctgcgcgtcg gcgagagcgc cggccaagag ctgcgcgacc
     1381 tgcggcgcga tctgcttgcg cagcacgtcg cgcagcgcgc ctttctcgtt cagcacctcg
     1441 cgcacccaga gcgcgggcca ccagggatgt ctcgcgacga cgtcatgcat gccgcgcacg
     1501 aacgcggaca ttagcgcacg cgagtccggg cccgccgcga ccaagccttc gcgcaagctt
     1561 gcggcgaccg gcgtcaggcg ctccgcgatg aacgcgtcga cgagcgcggc cttgctgccg
     1621 aagtagtagt tgacgagcgc cggcgtgacg ccggccttca gcgcgatcgc gcgcaggctc
     1681 gtcgccgcga tgccgtcgcc ggcgaagcag tcgacggcgg cgtcgagcag ccgctcgcgc
     1741 tggttcgctt cgtcggcgcg cgggcggccg ggacggcgag gttccggttt cctgcgccta
     1801 gtcaatttcg cgctcatgca gttaaattaa atcagcgtat aaataatcac aaggcggccg
     1861 tgacggtgtg ctaggctggc agccgaatcg agtcgccccg gcgcgtcgca accccatgtt
     1921 tcggcgcgga aaagcccaat tgctcagagc ttcgatcaag cgccgcgcgt tccttgcggc
     1981 cgcggccggc atggccggcc tcacggcgtt ccgcgcgggc aggacggcgg cgcagagcgc
     2041 gccggtccgc gtcacgcgcg aggagcccat catttcgcgc acgcagttcg atccggcccg
     2101 gccgccgccc gacatgccgg agctgacgcc gcccgaggcg ggcgtctgca ggacgacgtt
     2161 cgagctctcg gccggcgtga cgtactcggc ggaacggttg tcgcgcacgg cggcgcgcat
     2221 ctccgtcgac gggctcgaca tcgtgacgcg cgtccggttc gaaattttca cggcacgagg
     2281 cgcgccgccg aagcttgccg cgcacgagga agggcaccgc tcgatcggcg agcactacta
     2341 caaggatgcg gcgcaaatcg ccgaggtgat cggcaagcgc ctgatcggca cgacgttcga
     2401 cggcagcggc gccgaccaag aggccgcgca gcgcaacgcg ttcgagaaag tcatcgccga
     2461 gatcgagcgc gactacatgg cgcgcatgcg cacgccgagc gccgccgcga acgagcgctt
     2521 cgacgagatc acgagccacg gcctcgacgc gatcgacgag gccgacgccg tcgcggtcgc
     2581 gatcgccgcc gtcggcgcgc gttgaagcgg cgcgctgtcc tctcgagcgc tcgagctcag
     2641 cgcggccagc ctttcacgag gacgtgcacg acgccgaaca cgacgagcgc gacggccacg
     2701 ccgatcgcga cgcgattgac gaacggcagc ccgcccgtga gacgccagca gagcgcagtc
     2761 gcgaacgcgg gcacgacgaa cacggcccac cgaagccacg gatctttcgt tcccggcccc
     2821 atcgtcatcc aaggcccgcg tggcggcgcc gagaggcggc accgccacgc ggctcgctca
     2881 gaagtagtcc tcgacgaacg cgctgaccga gtactcgatg ccgccgagca gcccgcccgc
     2941 cagcgcgccc gggcccgcgc cgacgcctgc cgccgccgcc ccggcgagcc cacccgcgag
     3001 cgcgccgccc gccacatgcc caaagaagcc gccccacgtg aattctccac cgccgacgag
     3061 cgccgtctct tccgccgtca gctctcgtaa cgtccctgca caagctgcgt tcatgactgt
     3121 tcctccttga atgaccggtt tggaatcgat gccctcgcaa ttccgcgagg gcagccagta
     3181 gagcgccgca ccgcgccgcg cgcagctcga atatctgggc aattcaacga gattcgcccg
     3241 cgggcagcga aaccgtgcgt cgcggcccag gcgcttccgc cacgatccgc ccggcatcga
     3301 ggcgcacgat gcgatcggcc gcggcaagcg tctcgcgccg gtgtgccgcg atgatccgag
     3361 tcatgccgag ctgcgcgacg gccgccgcga tcgccgcctc gcgcggcgca tcgagatggc
     3421 tcgtcgcctc gtcgagaatc aacacggcgg gctgcgcgta cagcgcgcgc gcgagcaaga
     3481 tgcgctgctg ctggccggcc gacagcgtcg agcccatgaa gccgacgagc gtgttgtagg
     3541 ccatgggcaa cttggcgatg tcggaatgaa tggccgcgag ccgcgcgcac tcctccacgc
     3601 ggcgctgatc ggcatcggcg gcgaagaaac tgatgttgtc cgcgatcgag cccgcgaaca
     3661 ggcaatcgcc ctgcatcacg cccgcgaccg tgcgctgcgc gcccgcgagc tcgcgcggcg
     3721 ccacgccggc gatcgcgatc tcgccggccg aaggcgcgag caagccgagc agcagcgaga
     3781 gcagcgtgct cttgccgctg ccggacgcgc cgacgagcgc gacgcactcg cccggcgcga
     3841 tctcgagatc gactccgtcg agcacgtagg gctcgtgctc gccgtaacgg aagcgcaagc
     3901 cgcgcgcgcg gatcgcgagg cccggcgtcg ccgccgagac gaggagcctg cccgagccgt
     3961 cgtgctgctc gggcgccgtc agcacgatgt cgccgagccg ctcgcaatgc agccgcagca
     4021 gccgcacctc ggcgagcttg tcgatcagcg tgctgacgcg cacggcgaac tgccgttgat
     4081 acgcgacgaa cgcgagcagc atccccgtcg acagcgcgcc ggagagaacc aggctcgcgc
     4141 cgaggtagac gacgagcacg ttctcgagcc cgagcgtgag cccgttcccg gcccgcagcg
     4201 cgagccccag ccgctgcacg cgcgcgccgg cgttgatctg ctcgacgagc aggccgagcc
     4261 acgcgccgcg ccgctcatcg ccgcgcgcaa agagcttgat tgcgcgaacg ccgcgtgccg
     4321 tctcgatgaa gtggctgtcc tgcttggccg ccgcggcgat ttgctcgtga cgcgcgtcgc
     4381 gcagtggcgc gtgcgcgacc cagcgaagcg ccgcgtagac cgcgatcgcg gcgacgcaga
     4441 cggccgcgag cggcgcgctg tagcagagca tcaccgcgag cagcagcagc gacattgcgc
     4501 cgtcgacgat cgcttcgacg aacgtcgtgg tcaccgtgcg ctgaatcacg tcgatcgagc
     4561 ggaaccgcga gacgatgtcg cccaggtgcc gcttctcgaa gtacggcaat ggcaggcgca
     4621 gcaagtgagc gagcacgttg gcttgccact gcacgttcag cgtcgcctcg aagtgcatga
     4681 tgacgacgcc gcgcagagcg gccgtcgact gctcgaacag caccagcaaa ccgaagccga
     4741 gcgcgagcag cgtcaagaga tcgcggctcg ccgtgacgag cgcatgatcg accacccact
     4801 gcatgagcag cggattcgcg agcacgaacg tctcgagcgt cgccgcgagc agcaggatct
     4861 gcgcgagcgc gcccgcgagc ccgcggacgt tgccgacgag cgcgcgaagc cgaacggccg
     4921 gcgccggcgc gcgcggcgtg aagcgcgggc tcggccacag ctcgagcgcg acgcccgtga
     4981 acgacgagcc ggcttccgcg cgccggacgc gccgaaagcc gcgcgccgga tcgtgcacga
     5041 gaaggccgcg ccgcgtcacg cggcgcaaga cgacgaagtg cgtgaagctc caatgcagga
     5101 tgcacggcag gcgcaagcgc gcgaggtcct cgagctcgac gcgcacggcg cgcgtctcga
     5161 ggccgaggcg ggccgcgagc tgcatcaatg cggccagcgt ggcgccgcgc atcgagaccg
     5221 tgaagcgccg ccgcagcgtc gcgacgtcga cggcgtggcc gtgatacgac gcgaccatcg
     5281 cgaggcacgc gacgccgcac tccgccgctt cggtttgcag cagcacgggc agcttacgcc
     5341 tcaggctcaa gcgcagccgc tcggcgagcg acatcggtag cctcctcgac aaccctgacg
     5401 ccgagcgcgc actagagcat gcggcccggc cgcatgcagg ccgcgtttcg cgcgatcttg
     5461 ccgagatacg cggtgcggag ctgcgcgtgc cggccgctag catcgagcgc atggaaagtg
     5521 acaacagcgc tccgctgccg ctcttccgtc ccgcggccat cgcggcgcag cgccgcgacg
     5581 agagcggcgg catcgtgctc acgcgtgcct ggtcgttcac gattttgagc gcgtttctcg
     5641 cggcgctggc cggcgctctg atctcgttcg cagtcttcgg cagctacacg gcgcatacga
     5701 cgctgcgcgg ccggctc
//
LOCUS       DASSNN010000337         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_803161, whole genome shotgun sequence.
ACCESSION   DASSNN010000337 DASSNN010000000
VERSION     DASSNN010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803161"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1761)
                     /gene="msbA"
                     /locus_tag="VFL84_06175"
     CDS             complement(7..1761)
                     /gene="msbA"
                     /locus_tag="VFL84_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HET7608245.1"
                     /translation="MPLNSKAADVYRKLLRYVVPYWPLVAAALAVMALQAGVNGAVPL
                     LTREVMERLHNGAQAGGSPWRIPLLLVLVFVLRGAMNFLGVYGLSWVGRGVIRRLRGE
                     LFRHYLVMPASVFDRSSSGDLISKLTYNTEQVAEAISTSMVTLVRDGLSIIVLLGVMV
                     YMSWQLTLLTAVVGPVIGLLVVQMSRAFRRHSARIQDSMGDVTRVAEQALQGHRVIKV
                     FEGQEYERSQFDKINRQNFRLNLRLVATRAAGDALTQYVVALGVAAVIFVAFSGSIFA
                     DLSAALFVGFLTAMAQLLTPLKRIININASSQRGIAAADSVFQILAEPVEPDAGAATI
                     ERARGDIEYRDVCFGYGTRAGRVLEHVSLEVPAGSTLALVGPSGSGKSTLASLLARFY
                     DVDAGTVLLDGKDIRSYRLRDLRRQLSLVSQDIVLFDDTIANNIAYGAPGEVSKDAIE
                     RAAEAAYVSEFARLLPEGLDTRVGERGVLLSGGQRQRIAIARALLKDAPVLILDEATS
                     ALDTESERRVQSALGNLMRGRTTLVIAHRLSTVENADRIAVMRDGAVVELGTHSELLA
                     REGLYAALHGAQVVNAAS"
     gene            complement(1875..>2086)
                     /locus_tag="VFL84_06180"
     CDS             complement(1875..>2086)
                     /locus_tag="VFL84_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7608246.1"
                     /translation="AYLVNGHPLVNSERRTLRLGIEKLIGKRRDMPVSIQADALATHQ
                     AVVTAMDVIGQLGFVNVNIATVTPP"
BASE COUNT          383 a          687 c          682 g          334 t
ORIGIN      
        1 ctgcgatcat gacgcagcgt tcacgacctg cgcgccatgc agcgcggcgt aaagaccttc
       61 gcgcgccaga agctcggaat gagtgccgag ctcgaccacg gcgccgtccc gcatcaccgc
      121 gatgcggtcc gcgttctcga cggtcgatag gcgatgcgcg atgacgagcg tggtgcggcc
      181 gcgcatgaga ttgccgagcg cgctctgaac gcgtctctct gattcggtat cgagcgccga
      241 ggtggcctcg tcgaggatca acaccggcgc gtccttgagc aaggcccgcg caatcgcgat
      301 gcgctgacgc tgaccgcccg agagcagcac gccgcgctcc cccacgcgcg tgtcgagacc
      361 ctcgggcagg aggcgagcga actcggaaac gtaggccgcc tccgcggcgc gctcgatcgc
      421 gtccttcgag acttcgcccg gagcgccgta ggcgatgttg ttggcgatcg tgtcgtcgaa
      481 cagcacgata tcctggctaa cgaggctcag ctgccgccgc aagtcgcgca gccgatagct
      541 gcgtatatcc ttgccgtcga gcagaacggt gcccgcgtcg acgtcgtaga accgggctaa
      601 caggctcgcg agcgtcgatt tgccgctgcc cgaggggccg accagcgcga gcgtcgagcc
      661 cgccggcacc tcgaggctga cgtgctcgag cacccggccc gcgcgggttc cgtagccgaa
      721 gcacacgtcg cgatactcga tgtcgccgcg agcgcgttcg atcgtggccg cgcccgcatc
      781 cggctcgacc ggctcggcga ggatttggaa cacgctgtcg gccgccgcga tgccgcgctg
      841 gcttgatgca ttgatgttga tgatgcgctt cagcggcgtc aggagctgag ccatcgccgt
      901 gagaaacccc acgaacagtg cggcgctgag atccgcgaaa atcgagcccg agaaggccac
      961 gaaaatcacg gcggcgacgc cgagggcgac gacgtactgc gtcaaagcgt cgccggccgc
     1021 acgagtcgcg acaaggcgca gattgagacg aaaattctgc cggttgatct tgtcgaactg
     1081 gctgcgctcg tactcctggc cctcgaatac tttgatgacg cgatgcccct gcagcgcctg
     1141 ctcggcgacc cgcgtcacgt cccccatcga gtcttgaatg cgggcgctgt ggcgtctgaa
     1201 cgctcggctc atctgcacca cgagcagccc gatgacggga cccaccacgg ccgtgagcaa
     1261 ggtcagctgc cagctcatat aaaccatgac gccgagcagg acgatgatcg agagaccgtc
     1321 gcgaacgagc gtgaccatcg aggtcgagat cgcctccgcc acctgttcgg tgttgtaggt
     1381 cagcttcgaa atcaggtccc cggaggagct ccggtcgaag acgctcgcgg gcatcacgag
     1441 atagtgccgg aagagttccc cacgcagcct tcgaatcacg cccctgccga cccagctgag
     1501 gccgtacacg ccgagaaaat tcatcgcccc gcgcagcacg aaaactaata cgagcagcag
     1561 cgggatgcgc cacggcgagc ctccggcctg cgcgccgttg tgcagacgtt ccatgacctc
     1621 gcgcgtcagc agaggcacgg cgccgttgac gccggcctgc agcgccatga cggcgagggc
     1681 cgctgcgacg agcggccaat acgggacgac gtatcgcagc agcttgcggt atacgtcggc
     1741 ggctttgctg ttcagcggca caccgcgcgc tcctttccga tcggccatac taatgatttt
     1801 atcgagtcgg cgtgcaagaa aggccggcga cggccgtgcc ggacgcggcg atcgcgccct
     1861 cgatggcagg aatcctaagg cggcgtgaca gtcgcgatgt tgacgttcac gaagccgagc
     1921 tggccgatga cgtccatggc agtcacgacc gcctgatggg tggcgagggc gtcggcctga
     1981 atgctgacag gcatgtcgcg ccgcttaccg atgagttttt ctatccccag ccgaagcgtg
     2041 cgccgctcgc tgttcacgag cggatggccg ttgacgagat acgcgc
//
LOCUS       DASSNN010000338         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1682224, whole genome shotgun sequence.
ACCESSION   DASSNN010000338 DASSNN010000000
VERSION     DASSNN010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682224"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFL84_06185"
     CDS             complement(<1..536)
                     /locus_tag="VFL84_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7608247.1"
                     /translation="MSGNEKHKWEVLLSARLWIAALMGFSSGLPLMLTLTVLQAWLTQ
                     EGVSLATIGMAGLVGLPYSIKFLWAPLVDRYKPLALGRRRSWLLITQIALAASIAFLG
                     MQSPGNNLWWLFTAALFVTFFSSTQDIVVDAYRRESLADEEQGLAASFYTYGYRIGML
                     LASAGGLILADIVGFNGV"
     gene            complement(533..1231)
                     /gene="trmB"
                     /locus_tag="VFL84_06190"
     CDS             complement(533..1231)
                     /gene="trmB"
                     /locus_tag="VFL84_06190"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019641312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET7608248.1"
                     /translation="METTLPGAAHMRKVRSFVRRPGRATAAQRRALAELLPRYGVGAA
                     TGPLDLAAIFGRDAPRVLDIGFGDGEALVTNALNNPGVGYVGVEVHEPGIGHLLLLLE
                     QARASNVRVIARDAAEVLPQLVADASFDAVDLLFPDPWPKKRHHKRRLVQPPFVAEVA
                     RVLKRGGFLHVATDWADYARHTREVLAQSEDLLPVEAADMCHNPLALRPPTKFERRGR
                     RLGHDVVDLFYGKR"
     gene            1363..1436
                     /locus_tag="VFL84_06195"
     tRNA            1363..1436
                     /locus_tag="VFL84_06195"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1395..1397,aa:Gly,seq:ccc)
     gene            1597..>2568
                     /locus_tag="VFL84_06200"
     CDS             1597..>2568
                     /locus_tag="VFL84_06200"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HET7608249.1"
                     /translation="MAENVNKLLIVEEDAVVVDAVGKAAKAAGYAIASAASGASFVQL
                     LESFHPTLIVMGARLPDTDGTALVTSLAARACTANVVLIGSGDGTAGEVHELGAAQGL
                     AMSGTLEKPLAAAELAAKLTTLLHRGTDLDADDLRRGLAGGEFTPHYQPKIARGAGGW
                     VVDGVEVLARWQHPRLGLVMPNRFIPLAERTGIIADLTSAMLNAALRQVRAWQEEGLR
                     LTCAVNLPPGLVTDLSFPDRVAESLAENGVEGNRLVLELTETATIQNPTATMAILARL
                     RVKGIGLSLDDFGTGYSSLTQLYQMPFSEMKIDKSLVMNVPQSREATT"
BASE COUNT          462 a          853 c          849 g          404 t
ORIGIN      
        1 tatacgccgt tgaatccgac gatgtcggcc aggatcaatc cgcccgccga agcgagcagc
       61 attccgatgc ggtagccgta cgtgtagaac gacgccgcga gcccttgctc ctcgtccgcc
      121 agcgattcgc gtcgatacgc atcgacgacg atgtcttgcg tcgacgagaa gaaggtgacg
      181 aacagggcgg cggtgaacag ccaccaaagg ttattgcccg gactctgcat gccgaggaag
      241 gcgatgctgg cggcaagcgc gatctgcgtg atcagaagcc agctgcggcg cctgccgagc
      301 gccaacggct tatagcggtc gacgagcggc gcccagagga acttgatgct gtacggcaat
      361 ccgacgagcc ccgccatgcc tatcgtcgcg aggctcacgc cttcctgagt aagccaggcc
      421 tgcagcaccg tcagcgtcag catcaacggc aagccgctcg aaaaacccat gagcgccgcg
      481 atccagagcc gcgcgctcag caacacttcc cacttgtgct tctcgttccc gctcatcgtt
      541 tgccgtagaa gaggtcgacg acgtcatgcc cgagccgtcg gccgcgccgc tcgaatttcg
      601 tcggcggtcg aagggcgagc ggattgtggc acatatccgc cgcttcgacg ggaagcaggt
      661 cctcgctttg cgcgagcacc tcgcgcgtgt gccgcgcgta gtcggcccaa tccgtcgcca
      721 cgtgcaggaa cccgccgcgc ttcaagacgc gcgcgacctc ggcgacgaac ggcggctgca
      781 cgagccggcg cttgtgatgg cgcttcttcg gccacggatc cggaaacagc aagtcgacgg
      841 cgtcgaagct cgcgtcggcg acgagctgcg gcaagacctc ggcagcgtcg cgcgcaatga
      901 cgcgaacgtt gcttgctcgg gcctgctcga gcagcagcag caaatggccg atgccgggct
      961 catgcacctc gacgccgacg tagcccacgc cgggattgtt caacgcgttc gtgacgagcg
     1021 cctcgccgtc gccaaagccg atgtcgagga cgcggggcgc gtcgcgaccg aagatcgccg
     1081 cgagatcgag cgggcccgtt gcggcgccga ccccgtaccg cggcaagagc tcggcgagcg
     1141 cgcggcgctg tgcggccgtc gcgcgcccgg gccggcgcac gaagctgcga accttgcgca
     1201 tgtgcgccgc gccggggagc gtcgtttcca taccggcgta agagtacccc gagcgtgcgg
     1261 cgacggtcgc agtcacggga acgggccggc ccggcaaaga cggctcgcgc aggcgcggcg
     1321 gaattgctta gaatctcacg gccagccaga gctatcgccg aagcgggtgt agttcaatgg
     1381 tagaacctca gcttcccaag ctgatggcgt gggttcgatt cccatcaccc gctccacttc
     1441 ctaagggcgt gagctcgaat cagccgtcga agattgacgg ccgtcacaca attcagccgt
     1501 cgcacagccg ctagctcgca gacgtctcta tccttggaat acggcgcaac ggttcgccca
     1561 ccggttcgtg cctttcatcc gaagtcgagc gcgtgcatgg ctgagaacgt caacaagttg
     1621 ctgatcgtcg aagaagacgc cgtcgtcgtc gacgccgtcg gcaaagccgc caaggctgcg
     1681 ggctatgcga tcgcgagcgc ggcgtccggc gcgagcttcg tgcagctgct cgagtctttt
     1741 cacccgacgt tgatcgtcat gggagccagg ctgccggaca cggatggcac ggcgctcgtg
     1801 acatcgctcg cggcacgtgc gtgcacggcc aacgttgtgc tgatcggtag cggcgacggc
     1861 acggccggtg aggtgcatga gctcggtgcc gcacaagggc tcgcgatgag cggcacgctc
     1921 gagaagccgc tcgccgcggc cgagctcgct gcgaagctca ctacgctgct ccaccgcggc
     1981 acggatctcg acgccgacga cttgcggcgc ggcctcgcag gcggtgaatt cactccgcat
     2041 taccagccga agatagcgcg cggcgcgggc ggctgggtcg tcgacggcgt cgaggtgctc
     2101 gcgcgctggc agcacccgcg cctcgggctc gtgatgccga atcgtttcat tcctctcgcg
     2161 gagcggacgg gcatcatcgc cgacctcacg agcgcgatgc tgaacgcggc gctgcgccag
     2221 gtccgcgcct ggcaagagga aggcctgcgc ctgacgtgcg ccgtgaattt gccgccgggt
     2281 ctcgtcacgg acttgagctt tccggaccgc gttgcggaat cgctcgccga gaacggcgtc
     2341 gagggcaacc ggctcgtgct cgagctcacg gaaacggcga cgatccaaaa tccgacggcg
     2401 acgatggcga tcctcgcacg tctgcgcgtc aaggggatcg gcctctcgct cgacgacttc
     2461 ggcaccggat actcgtcgct gacgcagctc taccagatgc cgttcagcga aatgaagatc
     2521 gacaagagcc tcgtcatgaa cgtgccgcaa agccgcgagg cgaccacg
//
LOCUS       DASSNN010000339         3406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_296861, whole genome shotgun sequence.
ACCESSION   DASSNN010000339 DASSNN010000000
VERSION     DASSNN010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296861"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VFL84_06205"
     CDS             complement(<1..671)
                     /locus_tag="VFL84_06205"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7608250.1"
                     /translation="MNALRLDELRFAVRRLRKDLGSTIASVVALACGIGAAVATWSLI
                     SAVFLNPLPVAEPARLFEVSGPRPSGVAEQWRPGHWYADLEAFRASGAFAGIAAVGER
                     QLGVVEQGNAEQDRDVRFAAHDFFSTLGIGAARGRTFAESDDRRGAPPVAVLSDRYWR
                     SVFNADPNVLGRTVTVAGTTATIVGVLPRGFRGLHLSQAPDLYLPLHVVSDMDRRQFN
                     ERDPLG"
     gene            complement(886..2562)
                     /locus_tag="VFL84_06210"
     CDS             complement(886..2562)
                     /locus_tag="VFL84_06210"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET7608251.1"
                     /translation="MKDDLAIARGVKLRKIVELAKERLGIEADDLEPYGHYKAKLSDA
                     LIARVQSRPDGKLILVTAITPTPAGEGKTTTTVGLGDALNAIGKKAMICVREPSLGPC
                     FGVKGGGAGGGHSQVAPMEDINLHFTGDFHAVSIAHNLLAALVDNHIYFGNQLGFDVR
                     RPIWRRVLDMNDRSLRHIVNGLGGTANGFPRETGYDIVAASEVMAILCLSSSLNDMRE
                     RFKQIIVGYTRDNKPIRAEALKAQGSMTVLMKEAIKPNLVQSLEHTPTLVHGGPFANI
                     AHGCNSVIATKTALKLADYVVTEAGFGADLGAEKFVDIKCRKSGLRPSAAVLVGTARA
                     LKYSGGAKIEDVNSEDTEALMKGLANMDRHVKNIRENYGLPVVVALNRFGSDTEREFE
                     LIKKHLGEQGVEALVCEHFAKGSKGATNLAKSMVKLIDTKPGSLKFVYEDGDELWRKI
                     GKVAKQVYHASEVVADTAVRQKIEKFQQDGFGNLPICIAKTQYSFSTDPKLRGAPEGH
                     VFNVRDVYLSAGAGFMVVLSGDMMTMPGLPKVPASDGIDIDADGNIVGLA"
     gene            complement(2629..>3406)
                     /locus_tag="VFL84_06215"
     CDS             complement(2629..>3406)
                     /locus_tag="VFL84_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET7608252.1"
                     /translation="LLFPAINVNDSVTKSKFDNLYGCRHSLVDAINRATDVMLAGKIA
                     VVAGYGDVGKGSAQSLRAQSARVVVTEIDPICALQAAMEGYTVMTMDEAAKIGDVFVT
                     TTGCVDVITEKHMKAMKHNAIVCNIGHFDNEIQVDKLKKYKWTEIKPQVHQVHMPNGK
                     SLILLAEGRLVNLGCATGHPSFVMSNSFTNQTLAQIELYQNTKKYPLGVYVLPKHLDE
                     KVASLHLEKIGVKLDKLSKQQADYLGVPVNGPYKSDHYRY"
BASE COUNT          559 a         1129 c         1080 g          638 t
ORIGIN      
        1 ccgagtgggt ctctttcatt gaactgccgg cgatccatgt cgctgacgac gtgcagaggc
       61 aaatagagat ccggcgcctg cgacaggtga agccctcgaa acccgcgcgg cagaacgccg
      121 acgatcgtcg ccgtcgttcc cgccaccgtc accgtgcggc ccaatacgtt cgggtcggca
      181 ttgaacacgc tgcgccagta gcgatccgac agtacggcca cgggcggtgc cccgcggcga
      241 tcgtcgcttt ccgcgaatgt tcggccgcgc gcggcgccga tcccgagcgt cgagaagaaa
      301 tcgtgcgcgg caaaccgtac gtcgcggtct tgctccgcgt tgccctgctc gacgacgccc
      361 aactgccgct cgcccacagc cgcgatgccc gcgaacgccc cgctggcgcg aaacgcttcg
      421 aggtccgcgt accagtgacc cggtctccac tgctcagcaa cgcccgaagg ccgcggcccg
      481 gaaacctcga acagccgcgc cggctccgcc acgggcagcg ggttcagaaa caccgccgag
      541 atcaacgacc aagtcgcgac ggcggcgccg attccgcaag ccagcgcaac gacggacgcg
      601 atcgtcgagc ccaagtcttt gcgtagccgg cgtaccgcga accgaagctc gtccaaccgc
      661 agcgcattca tgacttcccc ccactccaga gattggccgg tcgcaatttc aaacgcgtgc
      721 ggccattcgc gaatggccgg agggcatgag gatagcagcc ggcggcgcgc gaagcccggg
      781 ggatcgatga acggtcgcgc agcggatctc gccgagcgcg cggccaaaac aaacgcggcg
      841 cgcccctctc gagacgcgcc gcgttgcgat aggaaggtag gaggcttacg cgagcccgac
      901 gatattgccg tcggcgtcga tgtcgatgcc gtccgaggcc ggaaccttcg gcaagcccgg
      961 catcgtcatc atgtcgcccg agagcacgac catgaatccg gcgcccgcgc tcaagtagac
     1021 gtcgcgcacg ttgaacacgt gcccttccgg cgcgccgcgc aacttcggat cggtcgagaa
     1081 cgagtactgc gtcttcgcga tgcagatcgg caggttgccg aagccgtctt gctggaactt
     1141 ctcgatcttc tgccgcacgg cggtgtcggc gacgacctcg ctcgcgtgat agacctgctt
     1201 cgcgaccttg ccgatcttgc gccagagctc gtcgccgtcc tcgtagacga acttgagcga
     1261 gcccggcttc gtgtcgatga gcttgaccat cgacttcgcg aggttcgtgg cgcccttcga
     1321 gcccttggcg aagtgctcgc agacgagcgc ttcgacgccc tgctcgccca ggtgcttttt
     1381 gatcagctcg aactcgcgct cggtgtcgct gccgaagcgg ttcagcgcga cgacgaccgg
     1441 gaggccatag ttctcgcgga tgttcttgac gtggcgatcc atgttcgcca ggcccttcat
     1501 cagcgcttcc gtgtcctcgc tgttgacgtc ctcgatcttc gcgccgcccg agtacttcag
     1561 cgcgcgcgcc gtgccgacca gcacggccgc cgacgggcgc aagccggact tgcggcactt
     1621 gatgtcgacg aacttctcgg cgcccaggtc cgcgccgaag cccgcttctg tgacgacgta
     1681 gtcggcgagc ttcagcgccg tcttcgtcgc gatcacggag ttgcagccgt gcgcgatgtt
     1741 cgcaaatggg ccgccgtgca cgagcgtcgg cgtgtgctcg agcgactgca cgagattcgg
     1801 cttgatcgcc tctttcatga gcacggtcat cgagccttgc gccttcagcg cttcggcacg
     1861 gatcggcttg ttgtcgcgcg tgtagcccac gatgatctgc ttgaagcgct cgcgcatgtc
     1921 gttcagcgac gacgacaagc acaggatcgc catgacctcg gacgcggcga cgatgtcgta
     1981 gcccgtctcg cgcggaaaac catttgccgt gccgccgaga ccgttcacga tgtggcgcag
     2041 gctgcggtcg ttcatgtcga gcacgcgccg ccagatcggc cggcgcacgt cgaagccgag
     2101 ctggttgccg aagtagatgt gattgtcgac gagcgccgcg agcaagttgt gcgcgatgct
     2161 gacggcgtgg aagtcacccg tgaaatgcag gttgatgtcc tccatcggcg cgacctgcga
     2221 atggccgccg ccggcgccgc cgcctttgac tccgaaacag gggccgagcg acggctcgcg
     2281 cacgcaaatc atggccttct tgccgatcgc gttcagcgcg tcgcctaagc ccaccgtcgt
     2341 cgtcgtcttg ccttcgccgg ccggcgtcgg cgtgatcgcg gtgacgagaa tcagcttgcc
     2401 gtcgggccgc gactgcacgc gggcgatcag agcgtcggag agcttcgcct tgtagtgacc
     2461 gtacggctcg aggtcgtcgg cctcgatgcc gaggcgttcc ttcgcgagct ccacgatttt
     2521 gcgcagcttg acgccgcgcg ctatggctag atcgtctttc atgcttgagt caccgcgttc
     2581 gagctgaaac ggaacgcccc ggccgaacgg ccggggcgtg gcttgcgatc agtaacgata
     2641 gtggtcggac ttgtacgggc cgttgactgg cacgcccaag taatccgctt gctgcttcga
     2701 cagcttgtcg agcttcacgc cgatcttctc gaggtgcaag ctcgcgacct tctcgtccaa
     2761 gtgcttcggc agcacgtaga cgccgagcgg gtacttcttc gtgttctgat agagctcgat
     2821 ctgcgccagc gtctggttcg tgaagctgtt cgacatcacg aacgagggat gccccgtcgc
     2881 gcagcccaag ttcacgaggc ggccttcggc gaggagaatt aggctcttgc cgttcggcat
     2941 gtgcacctgg tgcacctgcg gcttgatctc ggtccacttg tacttcttga gcttgtcgac
     3001 ctggatctcg ttgtcgaagt ggccgatgtt gcagacgatg gcgttgtgct tcatcgcctt
     3061 catgtgcttc tcggtgatga cgtcgacgca gcccgtcgtc gtgacgaaca cgtcgccgat
     3121 cttcgcggcc tcgtccatcg tcatgaccgt gtagccttcc atcgccgcct gtagcgcgca
     3181 gatcgggtcg atctcggtca cgaccacgcg cgccgactgc gcgcgcagcg actgcgccga
     3241 gcccttgccg acgtcgccgt agccggcaac gaccgcaatc ttgccggcca gcatgacgtc
     3301 cgtcgcgcgg ttgatcgcgt cgacgagcga gtggcggcag ccgtagaggt tgtcgaactt
     3361 cgacttcgtc acggagtcgt tgacgttgat cgccgggaac aggagt
//
LOCUS       DASSNN010000340         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_296877, whole genome shotgun sequence.
ACCESSION   DASSNN010000340 DASSNN010000000
VERSION     DASSNN010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296877"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="VFL84_06220"
     CDS             <1..1472
                     /locus_tag="VFL84_06220"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HET7608253.1"
                     /translation="MRAPIALSKTKLLSLTQCRRKLWLETYSPELAPEPSPEKTALLA
                     TGNAVGEIARQLYGRGLGTVVSFERGLRAAIDATRALVEGGGREPIFEATFDHDGVAV
                     RIDVLDRSEPRVKIVEVKSSARVKDHHIDDCAIQAWALAQNGVGVRQIAVATLNPDFV
                     YAGDGRYEGLLVETDVTERVQERSKSVGELVVAARRTLAALDEPAVAVGMHCGAPHEC
                     DFYAHCAPPAGKYAVLALGGSKEKLYELMHAGFTDVRDVPEDKLTSDVQRRIWQQSRL
                     EAPYVGAELRDFVGALAFPRYYLDFETIGPAVPLFAGTRPFEALPFQWSCHIERSRGA
                     IEHAEFLELDGGAPMRAVAESLLATLGTAGPILVYTPYERRVLNDLAARYDDLAAGLL
                     ALAERIVDLHPLVRRHYYHPAMHGSWSIKAVLPTVAPDLSYAKLGAVQDGLAAQAAYL
                     EAIDAKTPPTRRAALRRALLDYCRHDTLALVRLVEFFGR"
     gene            1503..>2476
                     /locus_tag="VFL84_06225"
     CDS             1503..>2476
                     /locus_tag="VFL84_06225"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7608254.1"
                     /translation="MRHSISVLACAALAAAAAFAPRARAHHAFATEFDAALEGEVKGE
                     VTRVWWQNPHIRYDVAMKMPDGSTQSWALLPPGNLPTYRRENWTEQTIQVGYQVTATG
                     NLGRDGTKKLYATCINVGSGPEKGRQLGRCATSGTVTQVTADPNVDYTVHANDYAVDI
                     TGYWDNRYKFQVTVDDFQPKPMPMTAETAAIYKGRKYGDDHVLRCLPAGLPRIFGSPY
                     PMQIVDAGTHYLMVFMQDNTPRRVFMDGRKPPAEQPLTSMGFSTGKWEGRTLVIETTM
                     LLPGWLDGSGYPMTGGDGTRIVERWTPSADGLTMERIMTVTDKGYTQPL"
BASE COUNT          414 a          854 c          840 g          368 t
ORIGIN      
        1 cgatgcgagc gccgattgcc ctttccaaaa cgaagctgct gtctctgacc cagtgccggc
       61 gcaagctctg gctcgagacc tatagcccgg agctcgcgcc cgagccgtcg cccgaaaaga
      121 cggcgctgct cgcgaccggc aacgccgtcg gcgagatcgc gcggcagctg tatggccgcg
      181 gcttgggaac cgtcgtcagc ttcgagcgcg gcttgcgcgc ggcgatcgac gcgacgcgag
      241 cgctcgtcga gggcggcggc cgggagccga tcttcgaggc gacgttcgac cacgacggcg
      301 tcgccgtgcg catcgacgtg ctcgaccgca gcgagccgcg cgtcaagatc gtcgaggtga
      361 agtcgtcggc gcgcgtcaag gatcaccaca tcgacgattg cgcgatccaa gcgtgggcgc
      421 tcgcgcagaa cggtgtcggc gttcggcaga tcgccgtcgc aacgttgaat cccgatttcg
      481 tttacgcggg cgacggacgg tacgaagggc tgctcgtcga gaccgacgtg accgagcgcg
      541 tgcaggagcg cagcaagagc gtcggcgagc tcgtcgtcgc ggcgcggcgc acgctcgccg
      601 ctctcgacga gcccgcggtc gcggtcggca tgcactgcgg cgcgccgcac gagtgcgact
      661 tctacgcgca ctgcgcgccc ccggcgggca aatatgcggt gctcgctctc ggcggcagca
      721 aggagaagct ctacgagctc atgcacgccg ggttcaccga cgtgcgcgac gttcccgagg
      781 ataagctcac gagcgacgtg cagcggcgca tctggcagca atcgcggctc gaagcgccgt
      841 acgtcggcgc ggagctgcgc gacttcgtcg gcgcgctcgc gtttccgcgt tactacttgg
      901 acttcgagac gatcgggccg gccgtgccgc tcttcgccgg cacgcggccg ttcgaggcgc
      961 tgccgttcca atggtcatgc cacatcgaga ggagccgcgg cgcgatcgag cacgccgagt
     1021 tcctcgagct cgacggcgga gcgccgatgc gcgcggtcgc ggaaagtctg ctcgcgacgc
     1081 tcggcacggc cgggccgatc ctcgtctata cgccctacga gcggcgcgtg ctgaacgatc
     1141 tcgccgcgcg ctacgacgat ctcgcggcgg gcttgcttgc gctcgccgag cgcatcgtcg
     1201 atctccaccc gctcgtgcgc cggcattatt accacccggc catgcacggc tcgtggtcga
     1261 tcaaagccgt gctgccgacc gtggcccctg atctgagcta cgcgaagctc ggcgccgtgc
     1321 aagacgggct ggccgcgcaa gcggcctatc tcgaggcgat cgacgcgaag acgccgccga
     1381 cccggcgcgc cgcgctacgc cgcgcgctgc tcgactactg ccgccacgac acgctcgcgc
     1441 tcgtgcggct cgtggagttc ttcggccgct aagggctaga ctactcggag ggaggtttcg
     1501 ccatgcgcca ctcaatctcc gtgctcgcct gcgccgctct cgcggccgca gcggccttcg
     1561 cgcctcgcgc gcgggcccac cacgcgttcg ccaccgagtt cgacgccgcc ctcgaaggcg
     1621 aggtcaaagg cgaggtcacg cgcgtttggt ggcaaaaccc gcacattcgc tacgacgtcg
     1681 cgatgaagat gccggacggc tcgacgcaga gttgggcgct gctgccgccc ggcaacttgc
     1741 cgacgtatcg gcgcgagaac tggaccgagc agacgatcca ggtgggctat caggtcacgg
     1801 cgacgggcaa tttggggcgc gacggcacga agaagctcta cgcgacttgc atcaacgtcg
     1861 gcagcggacc cgagaagggc cggcagctcg gccgctgtgc gacgagcggc accgtcacgc
     1921 aagtcacggc cgacccgaac gtcgactaca cggttcacgc caacgactac gccgtcgaca
     1981 tcacgggcta ctgggacaac cgctacaagt tccaagtcac cgtcgacgac ttccagccga
     2041 agccgatgcc gatgacggcc gagacggcgg cgatctacaa aggccgcaag tacggcgacg
     2101 atcacgtgct acgctgcctg cccgcaggcc tgccgcgcat cttcggctcg ccatacccga
     2161 tgcagatcgt cgacgcgggc acgcactact tgatggtgtt catgcaggac aacacgccgc
     2221 ggcgcgtctt catggacggc cgcaagccgc cggccgagca gccgttgacg tcgatgggct
     2281 tctcgacggg caagtgggaa ggacgcacgc tcgtcatcga gacgacgatg ctgctgcccg
     2341 gctggctcga cggctcgggt tatccgatga cgggcggcga cggcacgagg atcgtcgagc
     2401 gctggacgcc ctcggcggac ggcttgacga tggaacgcat catgaccgtg accgacaagg
     2461 gctacacgca gccgct
//
LOCUS       DASSNN010000341         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_669264, whole genome shotgun sequence.
ACCESSION   DASSNN010000341 DASSNN010000000
VERSION     DASSNN010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669264"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2116
                     /locus_tag="VFL84_06230"
     CDS             <1..2116
                     /locus_tag="VFL84_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HET7608255.1"
                     /translation="VNNEDLRDLQNAAAADGGAEDTAPAAGAADPGVDVESEQLFADE
                     QTPPEPEQTEAPVATPTPAPVAAPVSTEPSLVSRALGWLASPLLWIGIGVAALLVISL
                     WFVRRRRHESEDVTGRWEALESEVDDDQETRDATERLRRQAPEETIVVEEQHAERLRP
                     VPVEPEPAPAPRRAAAPRPQKSVSPDETLSSQTVINLDQADAVAEADFHIAYGLYDQA
                     AELVQKALEAAPDRRDLKLKLLEVYFMWGNKESFLKAAESLRADIGDSDDADWNKVVI
                     MGKQICPDERLFTEAKAGGGARVDVDLEAGDSPLDLAFDDTASADIAAASSGLDLDLE
                     ASDQRAAPKAAKAKSAGKAAGRGNADDDSLDIGARTAAGLEAALFADDEPASQTTPDL
                     SDSLAVTQESPTIERPGAGRWSTEDAGDLSTHDSPTVETPTIESLGPKSSSTIKSKTV
                     AMPRRTDSTVEQPGLAASTGEFTAEIDLDDLGLDVKDIEGLPGDLGDLPLAGGQSDTR
                     EQPALGDDDGLLSATGVTKVLHGAEADEDSTESNTSVLEDQDATMLSSTLTGTEVLEG
                     RRKGADNSGNTSLVKQLGGVKDDAVDLDLADLTAALHGADTVEQPRSFSRDVFGTGST
                     PLDLDVGNGLATDDDDPTGTAEQALDPQTMTEVGTKLDLARAYIDMGDPEGARSILQE
                     VLDEGDSNQRREAQGLIDALHA"
     gene            2187..>2471
                     /locus_tag="VFL84_06235"
     CDS             2187..>2471
                     /locus_tag="VFL84_06235"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HET7608256.1"
                     /translation="MPRIALGIEYDGTDFVGWQIQQTGRSVEGTLAAAVSFVAGAPVI
                     VHGSGRTDAGVHALHQVAHFDTAVTRTPRQWLLGINSNLPADVAIRWVREV"
BASE COUNT          427 a          848 c          857 g          339 t
ORIGIN      
        1 ggtcaacaac gaagacttgc gcgatctgca gaacgcggcc gccgccgacg gcggggccga
       61 agacaccgcg ccggccgcgg gcgccgcgga tcccggcgtc gacgtcgagt ccgagcagct
      121 gttcgccgac gagcaaacgc cgcccgagcc cgagcaaacc gaggcccccg tcgccacgcc
      181 gacgccggcg cctgtcgcgg ctccggtctc gacggagccg tcgctcgtct cgcgggcgct
      241 cggctggctc gcttcgccgc tgctctggat cggcatcggc gtcgcggcct tgctcgtgat
      301 ttcgctctgg ttcgtgcgcc gccggcgcca tgagtccgag gacgtcacgg gccgttggga
      361 agcgctcgaa tcggaggtcg acgacgatca ggagactcgc gacgcgacgg agcgcctgcg
      421 ccgtcaagct cccgaagaga cgatcgtcgt cgaggagcag cacgcggagc ggctgcgtcc
      481 cgtgccggtc gagccggagc cggccccagc cccgcgccgc gcggctgcgc cgcggccgca
      541 gaaatcggtc tcgccggacg agacgctgtc gagccagacc gtcatcaatc tcgaccaagc
      601 cgacgccgtc gcggaggcgg acttccacat cgcgtacggc ttgtacgacc aagccgccga
      661 gctcgtgcag aaagcgctcg aggccgcgcc cgatcggcgc gacttgaagc tcaagctgct
      721 cgaggtctat ttcatgtggg gcaacaagga gtcgttcctc aaagccgccg agagcctgcg
      781 cgccgacatc ggcgactcgg acgatgccga ctggaacaag gtcgtcatca tgggcaagca
      841 aatttgcccg gacgagcgct tgttcacgga agcgaaagcc ggtggcggcg cccgcgtcga
      901 cgtcgacctc gaagccggcg actcgccgct cgatctcgcg ttcgacgaca ccgcgtcggc
      961 cgacatcgcg gccgcgagca gcgggctcga cctcgatctc gaggcgtcgg atcagcgcgc
     1021 ggcgccgaag gcggccaaag ccaaatcggc cggcaaggcg gccgggcgcg gcaacgccga
     1081 tgacgactcg ctcgacatcg gcgcgcgcac cgcggcgggt ctcgaggccg ctttgtttgc
     1141 ggacgacgag cccgccagtc agacgacgcc cgacctatcg gacagccttg cagtgactca
     1201 ggagtcgccg acgatcgagc ggcccggcgc cggccgctgg tcgacggagg acgcgggcga
     1261 tctttcgacg cacgattcgc cgaccgtcga gacgccgacg atcgagagct taggtcccaa
     1321 atcttcgtcg acgatcaagt cgaagaccgt cgcgatgccg cggcgcaccg actcgaccgt
     1381 cgagcagccc gggctcgcgg cttcgaccgg cgagttcacg gccgagatcg acctcgacga
     1441 cttggggctc gacgtcaagg acatcgaagg cttgccggga gacctcggcg acctgccgct
     1501 ggcaggcggc cagagcgaca cgcgtgagca acccgcgctc ggcgacgacg acggcctctt
     1561 gtcggcgacg ggtgtcacga aggttctgca tggcgcggag gccgacgaag attcgacgga
     1621 gagcaacacg tcggttctcg aggatcagga cgcgacgatg ctgtcgagca cgctgacggg
     1681 gaccgaagtg ctcgaagggc ggcgcaaagg cgccgacaac tcgggcaaca cctcgctcgt
     1741 gaagcagctc ggcggcgtga aagacgacgc ggtcgatctc gatctcgccg acctgacggc
     1801 cgcgctgcac ggcgccgata ccgtcgagca gccgcgctcg ttctcgcgcg atgtgttcgg
     1861 caccggctcg acgccgctcg atctcgatgt cggcaacggc cttgccaccg acgacgacga
     1921 cccgaccggg accgcggagc aggcgctcga tccgcaaacg atgaccgagg tcggcacgaa
     1981 gctcgatcta gcgcgggcgt acatcgacat gggcgacccg gaaggcgcgc gcagcatcct
     2041 gcaagaggtg ctcgacgaag gcgattcgaa ccagcgccgc gaggcgcagg gcttgatcga
     2101 cgcgttgcac gcgtagacgc tgattttgcc gccgccgcgc cgccatccat ggcggccgtc
     2161 gcgttttgaa gatcggcggc agctttatgc cgagaatcgc actggggatc gaatacgacg
     2221 gcaccgattt cgtcggctgg cagatccagc agacgggccg cagcgtcgaa ggcacgctcg
     2281 cggccgccgt gagcttcgtc gccggcgccc ccgtcatcgt gcacggctcg ggcaggaccg
     2341 acgccggcgt tcacgcgctg catcaagtcg cgcatttcga cacggccgtc acgcggacgc
     2401 cgcggcaatg gcttctcggc atcaactcga atttgccggc cgatgtcgcg attcggtggg
     2461 tgcgcgaggt g
//
LOCUS       DASSNN010000342         3697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_63850, whole genome shotgun sequence.
ACCESSION   DASSNN010000342 DASSNN010000000
VERSION     DASSNN010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63850"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VFL84_06240"
     CDS             complement(<1..598)
                     /locus_tag="VFL84_06240"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET7608257.1"
                     /translation="MLTKSFSRRALLLGFGLAAGAIGLGDAGPAAAQEPLKIGIIGTG
                     RIGGALARHWVKAGHEVFMSSRHPEELEPLAKELGPKAHVGQPREAAAFGSVVLVSVP
                     YGAMPQIGADFAAELAGKVIIDTSNPVANRDGPQVAEWQSKGAGVSTAGLLKNNRVVR
                     AFNCIPAQSLAERANRQPDRIAIPIGGDDAAALAIAERL"
     gene            complement(639..1124)
                     /locus_tag="VFL84_06245"
     CDS             complement(639..1124)
                     /locus_tag="VFL84_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007818998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7608258.1"
                     /translation="MPVIAAKLEALGLVLPKAVRPPPGITLPFRLVRVRGERAYVSGH
                     GPLNPDGTLAPPLGKVGRDLSVEQGYRAARLTALAMLGSIERELGDLDRVRAWTRVFG
                     MVNSATGFNQQPRVMNGFSDLILELFGPDRGAHSRSAVGMAELPFDLPVEIEAELEIE
                     P"
     gene            complement(1134..1331)
                     /locus_tag="VFL84_06250"
     CDS             complement(1134..1331)
                     /locus_tag="VFL84_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608259.1"
                     /translation="MTRNARYVVNAVVLLLIGAHGIYRFASERALLDADGLALGLVVA
                     QIVVGIGGAIWFFMRSRSVSG"
     gene            complement(1342..1806)
                     /locus_tag="VFL84_06255"
     CDS             complement(1342..1806)
                     /locus_tag="VFL84_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7608260.1"
                     /translation="MNSVVDDLVSRFDQGTLSRRHLLQGLAALTAAGGAGDAMAQTAP
                     FKSTRIDHISIQVTDMARSVEFYEKIFGLKVLNEDKENEIVRMGVTRIIVSLHRKAPV
                     GIVDHFAIAIDDFNRDAVTAELAKHGLTAEQNLDYGFFVRDPEGVPVQIVGT"
     gene            complement(1964..>3697)
                     /locus_tag="VFL84_06260"
     CDS             complement(1964..>3697)
                     /locus_tag="VFL84_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7608261.1"
                     /translation="DAAGNLTTPLLPEPGVSAADYGIAPAEQAAREQRVAQMRELLAQ
                     NQLVRALRDEPVAEERPAAAAEPLEREAFNELDGGAVGGERQRRQALAERSVSPPASS
                     PPAPSAQANFDRLAKEVQAPAEADAPARDTASERADEARDAVVSALRRLDEPAVDSRG
                     KRLEQALAVEPISPKLGALGHADANAPKAEVAVRTFASEIDPFELGVLDTGHLVLFRN
                     VWREGQRYVQGALIDREVFAAAAIEKPYRASSVAGTSRLTVAFQGRVLERFAADASAE
                     LYSSSRGQLTGTVLHRARLLPPFGDVELTFLVDRLPRAPGTALLAWVAVTLGAVLIGG
                     FYLMYRFGAGQMRLARQQQDFVSAVSHELKTPLTSIRMYGEMLKEGWADDAKRQTYYD
                     YIHGESERLSRLIENVLQLARLTRNTQRFDLKRVTVAELMDVARSKVATQVERAGFTL
                     VVRDETPEGTAVVVDLDCAAQIFINLVDNALKFSAGAAAKKVEITARRSGDAAVLLTV
                     RDYGPGVPKTQLKKIFELFYRAENELTRETVGTGIGLALVRQLAAAMGARVDARNCEP
                     GAEFRVAFPPR"
BASE COUNT          587 a         1260 c         1268 g          582 t
ORIGIN      
        1 tcagccgctc ggcaatcgcg agcgctgccg cgtcgtcgcc gccgattggg atcgcgatgc
       61 ggtcgggctg ccgattggct cgctccgcga gcgattgcgc cggaatgcag ttgaacgcgc
      121 gcacgacgcg attgttcttg agcagcccgg ccgtggaaac gccggctcct ttgctctgcc
      181 actcggcgac ttgggggccg tcgcggttcg cgaccgggtt gctcgtgtcg atgatgacct
      241 tgccggcgag ctccgccgcg aaatcggcgc cgatctgggg catcgcaccg taaggcacgg
      301 agacgagcac gacggagccg aatgccgcgg cttcgcgcgg ctggccgacg tgcgctttgg
      361 ggccgagctc cttcgcgagc ggctcgagct cctcgggatg acgcgacgac atgaaaacct
      421 cgtgcccggc cttgacccag tggcgcgcga gcgctccgcc gatccggccc gtgccgatga
      481 tgccgatctt cagcggctcc tgcgcagcag cgggcccagc gtcgccgagc ccgatggcgc
      541 cggcggctag accgaagccg aggagcagcg cgcgacgaga aaacgacttc gtgagcatgg
      601 cgttccttca gtcgaggtgt gtcggtgacg atgatagctc agggttcgat ctcgagctcc
      661 gcttcgatct cgaccggcag atcgaacggc agctccgcca tgccgacggc gctgcgcgaa
      721 tgcgcgccgc ggtccggacc gaacagctcg aggatcaaat ccgagaaccc gttcatgacg
      781 cgcggctgct gattgaagcc cgtcgccgaa ttcaccatgc cgaagacgcg cgtccaggcg
      841 cgcacgcgat cgaggtcgcc gagctcgcgc tcgatgctgc cgagcatcgc tagcgccgtg
      901 agccgggccg cgcggtaacc ctgctcgacg gacaagtcgc ggcctacttt gccgagcggc
      961 ggcgccagcg tgccgtcggg attcagcggg ccgtgccccg acacgtaggc acgctcgccg
     1021 cgaactcgca cgaggcggaa cggcagcgtg atgcccggcg gcggccgaac ggctttcggc
     1081 aacacgaggc ccagcgcttc gagcttcgca gcgatgaccg gcatgacggc ccctcaaccc
     1141 gaaacgctgc gcgagcgcat gaagaaccag atcgcgccgc cgatgccgac gacgatctgc
     1201 gcgacgacga gcccgagcgc gagcccgtcg gcgtcgagca gcgcgcgctc gctcgcgaat
     1261 cgatagatgc cgtgcgcgcc gatcaaaagc agcacgacgg cgttcacgac gtagcgcgcg
     1321 ttcctcgtca tggcggtgct atcaggtgcc gacgatttgc acgggcacgc cttcgggatc
     1381 gcgcacgaag aacccgtagt cgaggttctg ctcggccgtg aggccgtgct tggcgagctc
     1441 ggccgtgacc gcgtcgcgat tgaaatcgtc gatggcgatc gcgaagtgat cgacgatgcc
     1501 gaccggcgcc ttgcggtgca gcgagacgat gatgcgcgtg acgcccatgc ggacgatctc
     1561 gttctctttg tcctcgttca ggaccttgag gccgaaaatc ttctcgtaaa actcgacgga
     1621 gcgcgccatg tcggtgactt gaatgctgat gtggtcgatg cgcgtcgact tgaacggcgc
     1681 cgtttgcgcc atggcgtccc cggcgccgcc ggcggcggtc aacgcggcca gcccctgcag
     1741 caggtggcgg cgcgacaagg taccctgatc gaagcgagag acgagatcgt cgacgacgga
     1801 attcatgccg cacctcccca agcggaccgg ttcgcgctca gtgtacgccg ccaacgaccg
     1861 caaaagggga ccgcaaaagg ggacagatct attttctgcg ctgcggagca gtcggatcca
     1921 ttctcctgcg cagaaaatag atctgtcccc ttttggaagc gccctaacgc ggggggaatg
     1981 cgacgcggaa ctcggcgccc ggctcgcagt tgcgcgcgtc gacgcgcgcg cccatcgccg
     2041 cggcgagctg gcggacgagc gcgagcccga tccccgtgcc gacggtttcg cgcgtcagct
     2101 cgttctcggc gcgatagaag agctcgaaga tcttcttcag ctgcgtcttc ggcacgccgg
     2161 gcccgtaatc gcggacggtc agcaatacgg ccgcgtcgcc gctccggcgc gccgtgatct
     2221 cgaccttctt ggcggcggcg cccgccgaga atttcagcgc gttgtcgacc aagttgatga
     2281 agatctgcgc cgcgcaatcg aggtcgacga cgacggccgt gccttcgggc gtctcgtcgc
     2341 gcacgacgag cgtgaagccg gcgcgctcga cctgcgtcgc gaccttcgag cgcgccacat
     2401 ccatgagctc ggcgaccgtg acgcgcttca agtcgaagcg ctgcgtgttg cgcgtgaggc
     2461 gcgcgagctg cagcacgttc tcgatcaggc gcgacaggcg ttcgctctcg ccgtggatgt
     2521 agtcgtagta ggtctgccgt ttcgcgtcgt cggcccagcc ttctttcagc atctcgccgt
     2581 acatgcggat ggacgtcagc ggcgtcttga gctcgtggct cacggccgag acgaagtctt
     2641 gctgttgacg cgcgagccgc atctggccgg ccccgaagcg atacattaga taaaagccgc
     2701 cgatcagcac ggcgccgagc gtgacggcga cccaagcgag cagcgccgtg cccggagccc
     2761 gcggcaagcg gtcgacgaga aacgtcagct cgacgtcgcc gaacggcggc aacagcctcg
     2821 cgcgatgcag caccgtgccc gtgagctgcc cgcgcgagga gctgtaaagc tcggcgctcg
     2881 catcggccgc gaagcgctcg agaacacgac cttgaaacgc gacggtcaaa cggctggtgc
     2941 cggcgacact gctcgcacgg tacggctttt cgattgccgc cgcggcgaac acctcgcggt
     3001 cgatcagcgc gccctgcacg tagcgctgac cttcgcgcca aacattgcgg aacaaaacga
     3061 ggtgccccgt gtcgagcacg ccgagctcga acggatcgat ctcgctcgcg aacgtgcgca
     3121 cggcgacctc ggccttcggc gcgttcgcgt cggcgtgacc gagcgcgccg agcttcggcg
     3181 agatcggctc gaccgcgagc gcttgctcca gacgcttccc gcgtgagtcg acggccggtt
     3241 cgtcgaggcg gcgcagcgcg gacacgacgg cgtcgcgcgc ctcgtccgct cgctcggagg
     3301 cggtatcgcg cgccggcgcg tcggcttcgg ccggcgcttg cacttccttg gcgaggcggt
     3361 cgaagttcgc ttgcgcggaa ggcgccggcg ggctcgacgc gggagggctc acgctccttt
     3421 cggccagggc ttggcgccgc tgccgttcgc cgccgaccgc accgccatcg agctcgttga
     3481 acgcttcgcg ctcgagcggc tccgctgcgg cagccggccg ctcttcggca accggctcgt
     3541 cgcgcagcgc gcgcacgagc tggttctgcg cgagcagctc gcgcatctgc gcgacacgct
     3601 gctcgcgcgc ggcctgctcg gccggcgcga tgccgtagtc cgctgccgag acgccgggct
     3661 ccggcagcag cggcgtcgtg agattgccgg ccgcgtc
//
LOCUS       DASSNN010000343         5838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1682519, whole genome shotgun sequence.
ACCESSION   DASSNN010000343 DASSNN010000000
VERSION     DASSNN010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682519"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VFL84_06265"
     CDS             complement(<1..178)
                     /locus_tag="VFL84_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET7608262.1"
                     /translation="MTSKTIKRAHPAMRDDIADLTTRRPVPGRDVEQIDPFLFLNHHG
                     PQVYAPRNRGLPFGP"
     gene            339..935
                     /locus_tag="VFL84_06270"
     CDS             339..935
                     /locus_tag="VFL84_06270"
                     /inference="COORDINATES: protein motif:HMM:NF043005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCE4755 family polysaccharide monooxygenase-like
                     protein"
                     /protein_id="HET7608263.1"
                     /translation="MNKWMLALPVAGLAAAIAPAVNAHFKLLEPAAWLAQDDRGDPQK
                     LAPCGGTLADPGEPTEAVTAVTGGSKLKIVVNETIFHPGHFRISLARKRNALPLDPAV
                     SMHETERGLRSQSAFIMTTPMPPVLVDNLWPHTERPAANWETEITIPNITCEGCQLQV
                     IQVMLEHPGVREGGFSYHHCAVLNITADPAKPNEDASW"
     gene            complement(982..1557)
                     /locus_tag="VFL84_06275"
     CDS             complement(982..1557)
                     /locus_tag="VFL84_06275"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7608264.1"
                     /translation="MSKLVSLKRAVPMVVTAAALVLLSAGVFAHHNSQAEFGAFGSDT
                     IAVEGTIVNINWANPHISIDIRTTGGELPAGQNWRLVSHPIQIMNDYGFVREEFAVGD
                     RVKLLGWKNVRGAPQIWPRAIQVNDGPLKSNLRFTDMIDIAKGTFEAMHIRPAANLNG
                     SPPARAGAETVEKLKEMGLIDEKGLMIWPPR"
     gene            complement(1568..1969)
                     /locus_tag="VFL84_06280"
     CDS             complement(1568..1969)
                     /locus_tag="VFL84_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608265.1"
                     /translation="MRQILVAAFVAAAVCVGLFLIEDRFDGDGTAATAEGVAASRAPL
                     SLRYTDEPAEDSWVAGSRGAGADDRVLSSAANSICFLTKIEVKGVQGPDDAGTCRVAV
                     DDFTGFWQVTAAVPEGSKSEIRCNARCLVWE"
     gene            complement(1994..3088)
                     /locus_tag="VFL84_06285"
     CDS             complement(1994..3088)
                     /locus_tag="VFL84_06285"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7608266.1"
                     /translation="MTREFRLGAVLAAVTIVALPAWGHHSVAAEFDTANRGELKGEIT
                     RVWFTNPHVRYQLTVRNADGSTEDWELQGGNVTNLLRQGWLEDSIRVGDSVTVSGAMG
                     RAGAKKLNISDIVTSAGVHLPQRTAAPARDRNAINASADKKYGYASGKNAYPVDITGP
                     WRNDYKWRVTVDDLEPKPVPLTAAGRKLFEATEPWHDYSLRCVAPGLPRIFGAPYTAS
                     IVDAGDHYLLIYLEHNTPRRIWMDGRTAPPDTPATPMGFSVGKWEGNVLVVETTHLSP
                     GWLDGSGLPMSGEGTRIVERYVPSEDRLSIDRTMTIYDPYYTQPLVRQRYSARDDAVD
                     LTEQAPCDPDSFYRDLSRNGRLEEHFDRQR"
     gene            complement(3194..4219)
                     /locus_tag="VFL84_06290"
     CDS             complement(3194..4219)
                     /locus_tag="VFL84_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7608267.1"
                     /translation="MRNHALVQLTLVRFREFLREPEALFWVFGFPLLLAAGLGLAFRN
                     TPAEVLTVGAVGTEPAAFESAPALAVEPFATIDEGLRALREGKVALLVERLEDGSVRY
                     HYDDTNPEGRDARMRADMAVQQRGGRTDPVAVHDELMREAGSRYIDFLIPGLLGMNLM
                     GGSIWSMGFAIVDARRRKLMKRLIATPMPRHYFLLSFLISRLAMLIVEVGAFLAFGVL
                     VFGVPIRGSLVLVAAICVLGSLAFGAIGLLLSSRAKTIEAASGLMNLTMLPMWILSGV
                     FFSSQRFPDAVQPIIAALPLTALNDALRATMLQSATAIEVAPQLGVLAVWLAICFPLA
                     LKLFKWR"
     gene            complement(4212..5147)
                     /locus_tag="VFL84_06295"
     CDS             complement(4212..5147)
                     /locus_tag="VFL84_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7608268.1"
                     /translation="MKMREAALRVRALRKAYKDVVAVGGLDLEVPAGECFGLLGPNGA
                     GKTTTIEICEGLTARDSGEVEVLGMRWETDARALKQRLGIQLQETQLSDKLTVEETVR
                     LFRSFFDNGPTPEHVIALVQLEEKRRSRVGTLSGGQKQRLALACALVGDPEVLFLDEP
                     TTGLDPQARRQLWDLIEEFKRAGRTIVLTTHYMDEAERLCERVAIMDHGKIIALGSPR
                     ELVASIGVEHVVEFSVDDAARLDAAALRAIDGVRDARIENGTVRMQVTALHRAVPALL
                     AELVRRNVKLAELRTHSATLEDVFVTLTGRHLRDA"
     gene            complement(5148..>5838)
                     /locus_tag="VFL84_06300"
     CDS             complement(5148..>5838)
                     /locus_tag="VFL84_06300"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HET7608269.1"
                     /translation="HAFSIQNGVLKNELLEAAFGPEHVLGALANMSGERNPSGEVLFT
                     RNVSLQIGAQRGGIPEAVRALAQTIDSSGVRCAAVPNIAAQEWSKFAAWVGMVGVSIT
                     TRAVTWKYLMDPDAALVLVRLVREVRELAARAGVAFTDDVVFPVATLCTLTEQASVRL
                     LNEIGADFRDRSPEHRLSAHQDVDAGRPLEVDETLGHAARTAAREGLSLPLLDTVYRI
                     AAAIDRTSRAR"
BASE COUNT          983 a         2002 c         1922 g          931 t
ORIGIN      
        1 gcgggccgaa cggcagaccg cggttgcgcg gcgcatagac ctgcggcccg tgatggttca
       61 ggaacaagaa tgggtcgatc tgctcgacgt cgcgccccgg cacgggccgt cgcgtcgtga
      121 gatccgcgat gtcgtcgcgc atggccggat gcgcgcgctt gatggtcttc gaggtcatgg
      181 ttgcactccg ttgcagcagc tccgctactt ggcgcggctc gccagcatac aggggcggcg
      241 caatgcttgg cacgccgtgg aacaaatctt gagagcatcg gccgagcggt gttcggctag
      301 aatgcgccga caataacaat cccacccggg ggtgctcctt gaacaagtgg atgctcgcgt
      361 tacccgtggc cggcttggca gctgcgatcg caccggccgt gaacgcgcat ttcaagctgc
      421 tcgagcctgc cgcgtggctc gcgcaagacg accgcggcga tccgcagaag ctggcgccgt
      481 gcggcggcac gctcgccgac cccggcgagc cgacggaagc cgtgacggca gtgacgggcg
      541 gcagcaagct caagatcgtc gtcaacgaga cgatctttca ccccggccac ttcaggatct
      601 cgctcgcgcg caagcgcaac gcgctgccgc tcgaccccgc cgtctcgatg cacgagacgg
      661 agcgcggctt acggtcgcag tcggcgttca tcatgacgac gccgatgccg ccggtcctcg
      721 tcgacaatct ctggccgcat accgagcggc ccgcggcaaa ctgggagacc gagatcacga
      781 tccccaacat cacgtgcgaa ggctgccagc tccaggtcat ccaggtgatg ctcgagcatc
      841 ccggcgtccg tgaaggcggg ttctcgtatc accactgcgc cgtgctgaac atcacggcgg
      901 acccggcgaa gccgaacgag gacgcgagct ggtaaaagcg aaacgcggcg acagaagggt
      961 aagtcgcgtc ccacgctcgg ctcagcgcgg cggccagatc atgaggcctt tttcgtcgat
     1021 gagccccatt tccttgagct tctcgaccgt ctcggcgccg gcgcgcgcgg gcggcgagcc
     1081 gttcaagttt gccgcgggcc gaatgtgcat cgcttcgaac gtgcccttgg cgatgtcgat
     1141 catgtccgtg aagcgcaggt tcgacttaag cgggccgtcg ttgacctgaa tcgcgcgcgg
     1201 ccagatttgc ggcgcgccgc gcacgttttt ccaaccgagc agcttgacgc ggtcgccgac
     1261 ggcgaactct tcgcgcacga agccgtagtc gttcatgatc tggatcggat ggctgaccaa
     1321 tcgccagttc tgtccggccg gcagctctcc gcccgtcgtg cggatgtcga tgctgatgtg
     1381 cgggttcgcc caattgatgt tcacgatcgt gccttcgacg gcgatcgtgt ccgagccgaa
     1441 cgcgccgaac tcggcctgcg agttgtgatg ggcaaaaacg ccggccgaca gcagcacgag
     1501 agccgcagcc gtcaccacca tcggtacggc gcgtttgagc gacacgagct tgctcacttt
     1561 ggcgctccta ttcccagacg agacaacgcg cgttgcagcg gatctccgat ttgctgcctt
     1621 ccggcacggc ggccgtgacc tgccaaaaac ccgtgaagtc gtcgacggcg acgcggcacg
     1681 tgccggcgtc gtcggggccc tgaacgccct tgacctcgat cttcgtcagg aagcaaatcg
     1741 aattcgcggc gctcgacagc acacggtcgt cggcgccggc cccgcgcgag ccggcgaccc
     1801 agctgtcttc ggccggctcg tcggtgtagc gcagcgagag tggcgcgcgc gacgcggcga
     1861 cgccttcggc cgtggccgcg gtgccgtcac cgtcgaatcg atcttcgatc agaaagaggc
     1921 ccacgcacac ggccgccgcg acgaaggcgg cgacgagtat ctgtctcacg gtctgccccc
     1981 gtcgtccaag cgcttaccgc tgtcgatcga aatgctcctc gagccgcccg ttcctcgaga
     2041 ggtcgcggta gaaactgtcc gggtcgcagg gtgcctgctc cgtcaagtcg acggcgtcgt
     2101 cgcgcgccga gtagcgctgc cttacgagcg gctgcgtgta gtacggatcg tagatcgtca
     2161 tcgtgcgatc gatcgagagg cgatcctcgc tcggcacgta gcgctcgacg atgcgcgtgc
     2221 cttcgcccga catcggcagc cccgagccgt cgagccagcc cgggctcaag tgcgtggtct
     2281 cgacgacgag cacgttgcct tcccacttgc ccaccgagaa gcccatcggc gtcgccggcg
     2341 tgtcgggcgg cgcggttcgg ccgtccatcc agatgcgccg cggcgtgttg tgctcgagat
     2401 agatcagcag atagtgatcg ccggcatcga cgatggaggc ggtatacggt gcgccgaaaa
     2461 tgcgcggcag acccggcgcg acgcagcgca gcgaatagtc gtgccaaggc tcggtcgctt
     2521 cgaagagctt gcgccccgcg gccgtcagcg gcaccggctt cggctcgagg tcgtcgacgg
     2581 tgacccgcca cttgtagtcg ttgcgccagg ggcccgtgat gtcgacggga taggcgttct
     2641 tgccgctcgc gtaaccatat ttcttgtcgg cgctcgcgtt gatcgcattt cgatcacgag
     2701 cgggcgcggc cgtcctctgc ggcaggtgca cgcctgcgga cgtcacgata tcgctgatgt
     2761 tgagcttctt cgcgccggcg cgccccatgg cgcccgacac cgtgacgctg tcgccgacgc
     2821 gaatcgaatc ctcgagccag ccttgacgca gcagattcgt gacgttgccg ccctgcagct
     2881 cccagtcctc ggtcgagccg tccgcgttgc gaaccgtgag ttgatagcgg acgtgcggat
     2941 tcgtgaacca cacgcgcgtg atctcgcctt tgagctcgcc cctgttcgcg gtgtcgaact
     3001 cggcggcgac ggagtgatgg ccccacgcgg gcaacgcgac gatcgtcacc gccgcgagca
     3061 ccgcgcccag tcgaaattcc ctcgtcatga ccgcccccca aaggccgaga gccccgagcc
     3121 ccggctcacc cgaattgtac tctcgtgcgc cggcgccgaa accgccggcc acggcggccg
     3181 cgccgagcgt gggctaccgc catttgaaca gcttcagcgc cagcgggaag catatggcca
     3241 gccaaaccgc gagcacgccg agctgcggcg cgacctcgat cgccgtcgcg ctctgcagca
     3301 tcgtcgcgcg cagcgcgtcg ttcaatgccg tgagcggcag cgccgcgatg atgggctgaa
     3361 ccgcgtccgg aaatcgctgg gacgagaaga acacgccgga taggatccac atcggcaaca
     3421 tcgtcaaatt catgaggccc gaggccgctt cgatcgtttt cgcccgcgac gaaagcagca
     3481 ggccgatcgc gccgaaggcg agcgagccga gcacgcagat ggccgcaacg agcacgagcg
     3541 acccgcggat agggacgccg aacacgagca cgccgaacgc aagaaaggcg ccgacctcga
     3601 cgatgagcat cgcgagccgc gagatcagga acgacagcag aaaatagtgc cgcggcatcg
     3661 gcgtcgcgat gagccgcttc atcagcttgc gccgccgcgc gtcgacgatc gcgaagccca
     3721 tgctccagat cgagccgccc atcagattca tgccgagcag gcccggaatc aggaaatcga
     3781 tgtagcgcga tcccgcctcg cgcatgagct cgtcgtgcac ggccacgggg tcggtgcgtc
     3841 cgcccctctg ctgcaccgcc atgtccgcgc gcatgcgcgc gtcgcgccct tcggggttcg
     3901 tgtcgtcgta gtggtagcgc acggagccgt cctcgaggcg ctcgacgagc aacgcgactt
     3961 tgccttcgcg caacgcccgt aggccttcgt cgatcgtcgc gaacggctcg accgcgagcg
     4021 cgggcgcgct ctcgaatgcg gcgggctccg tgccgacggc gccgacggtc agcacctcgg
     4081 ccggcgtgtt gcggaacgcg aggccgaggc ccgccgcgag cagcagcgga aaaccgaaca
     4141 cccagaacag ggcttccggc tcgcgcagaa actcgcggaa ccgcacgagc gtcagctgca
     4201 cgagcgcgtg gttacgcatc gcgcaggtgc cgccccgtca gcgtgacgaa gacgtcctcc
     4261 aaggtggccg aatgcgtgcg cagctccgcg agcttgacgt ttcgacgcac gagctcggca
     4321 agcagcgccg gcacggcacg gtgcagcgcc gtgacttgca tccgcaccgt gccgttctcg
     4381 atgcgcgcgt cgcgcacgcc gtcgatggcg cgcaaagcgg cggcatcgag ccgcgccgca
     4441 tcgtcgaccg agaactcgac gacgtgctcc acgccgattg acgcgacgag ctcgcgcggc
     4501 gacccgagcg cgatgatctt gccgtggtcc atgatcgcga cgcgctcgca gagcctttct
     4561 gcttcgtcca tgtagtgcgt agtcagcacg atggttcttc ccgcgcgctt gaactcctcg
     4621 atgaggtccc agagctggcg gcgcgcctgc gggtcgagac ccgtcgtcgg ctcatcgagg
     4681 aacaagacct cggggtcgcc gacgagcgcg caggccaatg cgagccgctg cttctggccg
     4741 cccgaaagag tgcccacgcg cgagcgccgc ttctcttcga gctgcactag cgcgatgacg
     4801 tgctctggag tcgggccgtt gtcgaagaag ctgcggaaga gacgcaccgt ctcctcgacc
     4861 gtgagcttgt cggaaagctg cgtctcctga agctgaatgc cgagccgctg cttcagcgcg
     4921 cgtgcgtcgg tctcccagcg catcccgagc acctcgacct cgcccgagtc gcgcgccgtg
     4981 aggccttcgc agatctcgat cgtcgtcgtc ttgccggcgc cgttcggccc gagcagcccg
     5041 aagcactcgc cggccggcac ctcgagatcg aggccgccga cggcgacgac gtccttgtac
     5101 gccttgcgca gcgcgcgaac gcgcaatgcc gcttctcgca tcttcattca cctcgcgcgc
     5161 gacgtccggt cgatcgcggc agcaatgcgg tagacggtgt cgagcagagg cagcgacagc
     5221 ccctcgcgcg cggcggtccg ggcggcgtgc ccgagcgtct cgtcgacctc gagcggccgg
     5281 cccgcgtcga cgtcctgatg cgccgacagg cgatgctcgg gcgaacgatc gcgaaaatcc
     5341 gcgccgatct cgttcaggag cctgaccgac gcctgttccg tgagcgtgca tagcgtggcc
     5401 acgggaaaga cgacgtcgtc ggtgaaggcc acgccggcgc gcgcggcgag ctcgcgcacc
     5461 tcgcgaacca acctgacgag gacgagcgcg gcgtcgggat ccatcaaata cttccacgtg
     5521 actgcgcgag tcgtgatcga gacgcccacc atgccgaccc aggccgcgaa tttcgaccac
     5581 tcctgcgccg cgatgttcgg cacggccgcg cagcgcacgc cggaggagtc gatggtttgc
     5641 gcgagcgcac gcacggcctc cggaataccg ccgcgctgtg cgccgatctg caagctcacg
     5701 ttgcgcgtga agagcacttc gcccgagggg ttccgctcgc cgctcatgtt ggcgagcgcc
     5761 ccgaggacat gctcgggccc gaaggccgct tcgagcagct cgttcttcaa cacgccgttc
     5821 tggatcgaga acgcatgc
//
LOCUS       DASSNN010000344         4804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1446509, whole genome shotgun sequence.
ACCESSION   DASSNN010000344 DASSNN010000000
VERSION     DASSNN010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446509"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VFL84_06305"
     CDS             <1..821
                     /locus_tag="VFL84_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7608270.1"
                     /translation="RGFFASFSMFGATTGTMLGSAVGALLTTVMSAEALASWGWRAAF
                     VSGIAVAVVGIVIRRGMLDNPSKTAVASPLAVAFRDHGWQVLRVLGLNVASAATYYTI
                     FVYAATWVSQTTTIERAVALNITTVTILTFLVVLPIAAWVSDRFGRKLMLCVGMSACL
                     LAAYPLLTAMHAGVVATTAPAQMAFGAVLAMQMAAIPAAMTEMFPHAVRVSAVSVGYG
                     LAYALFGGTAPAVATWLINRTGNDTVFAWYLMGLTAVSLVLALSVKDRRGQPLP"
     gene            complement(828..1430)
                     /locus_tag="VFL84_06310"
     CDS             complement(828..1430)
                     /locus_tag="VFL84_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7608271.1"
                     /translation="MLRPSTLVWAFAAVVAACSGPPATTPQGNVAPSAAPAPVPGTHP
                     SWTGVTEPEEVIEARRALMDQAENLMKPIDSFTVGEPADANVLRANAATIGAMLHAVP
                     HLFPPTTNRFDPGAHDPPTLALPAIWERFAAFETFAENAERMAEALETSEDGEPLREA
                     SQRLRAACDACHAGFMKPYTPPVVTQEDKEFDFDSVLPPQ"
     gene            1542..2339
                     /locus_tag="VFL84_06315"
     CDS             1542..2339
                     /locus_tag="VFL84_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET7608272.1"
                     /translation="MFRFLPPTALLVAVLCAAKSNAQAPERATRLPSISPAHCVATLD
                     RREQADALRCPAALRNAIVEARDVCRDAGGKLAGLPEGDVWAIDVNGDGRSELAFDLE
                     SNVSCTDAWSVFSCGSLGCPRALYELRAGAWSVVGGISAASPEQLSLGAARSADGHRA
                     LEVCSQDRCAERWTYDWQGKSYEVTHADVRGARVEIAGSAQGLHALNAATPVRATPRA
                     NGEEVGRYDAGTEVVIIGTVEGGDWYYVSPCNACKSGFAPKSAVAVD"
     gene            complement(2359..3621)
                     /locus_tag="VFL84_06320"
     CDS             complement(2359..3621)
                     /locus_tag="VFL84_06320"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7608273.1"
                     /translation="MRHADVRRAAAASLLVFAIATARAQPPQVWPLDPQKLPGAIAGI
                     PAGTSWPSPPLGPGPFLVESVRSEHRNLRVVVVARGLEQPWSIAFLPGGDMLVTERAG
                     RLRLIKGGKLDPAPVAGIPAVRSRGLQGLMDVVLHPRFADNHFVYFSYHRPAGEDAGE
                     TMLARGRWENDKLVDVRDIFATGATGTEGSRIGFAKDGMLHMTVSAPGVGPDVIRSQN
                     PKDYAGKTIRLRDDGSIPPDNPFVNDARYLPAIYTLGHRNGHSMVLNPWTGELWATEQ
                     GPNGGDEINILQKGGNYGWPLVSYGRVYSGPRVSANPTLEGTIQPIVFWVPSIAVTGL
                     TFYQGDVFTAWRRSAFVGGLRQGEVPRTGQLQRIEFNDRWEEIRRETLLRELGQRIRD
                     VREGPDGLLYVLTAEAPDGAVLRIEPAS"
     gene            3723..4103
                     /locus_tag="VFL84_06325"
     CDS             3723..4103
                     /locus_tag="VFL84_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7608274.1"
                     /translation="MNKELFEKGLAIRKSVIGAEFVDKALATADDFNMPLQELVTEYC
                     WGAVWGREGLEKKTRSLLNIAMLSTLNRPHELKVHVKGAIRNGATKTEIREVLLQVAI
                     YAGVPAAVDSFRVAKEALAELEKK"
     gene            4264..>4804
                     /locus_tag="VFL84_06330"
     CDS             4264..>4804
                     /locus_tag="VFL84_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7608275.1"
                     /translation="MKVLVTGGAGFTGVHLVRALLDRGDDVTVLDLSEGDSLDTEPLK
                     ARGAKFIIGSVADKAALRNAVRGQEVIFHLASAFRDIHRGAPLFQEVDVEGTRRLLQE
                     ARLAGTRRVVHCSTQGVHGSLPAGTVPGNEESPIAPIDYYCEAKVAAEAVCDEFIADG
                     MDIVNVRPTSIYGPGDTHGW"
BASE COUNT          751 a         1635 c         1632 g          786 t
ORIGIN      
        1 gccgcggctt cttcgcgagc ttctcgatgt tcggcgcgac gacgggcacg atgctcggct
       61 ccgcggtcgg cgcgctgttg acgaccgtga tgagcgcgga ggcgctcgcg agttgggggt
      121 ggcgcgcggc attcgtctcc ggaatcgcgg tcgcggtggt cggaatcgtc atccgccgcg
      181 gcatgctcga taacccaagc aagaccgccg tcgcgtcgcc gctcgccgtc gcgtttcgcg
      241 accacggctg gcaagtgctg cgcgtgctcg ggctcaacgt cgcttcggcg gccacgtact
      301 acacgatctt cgtctatgcc gcgacctggg tatcgcaaac gacgacgatc gagcgcgccg
      361 tcgcgctcaa tatcacgacg gtcacgatcc tgacgttcct cgtcgtgctg ccgatcgcgg
      421 cttgggtttc ggaccgcttc ggccgcaagc tcatgctctg cgtaggaatg agcgcgtgcc
      481 tgctggccgc ctatccgctg ctcactgcga tgcacgcggg tgtggtcgcg acgacggcac
      541 ccgcgcagat ggcgttcggc gccgtgctgg cgatgcagat ggcggcgatc ccggcggcga
      601 tgaccgagat gtttccgcac gcagtgcgcg tcagcgccgt cagcgtcggc tacgggctcg
      661 cgtacgcact gttcggcggc acggcaccgg cggtcgcgac ttggctcatc aaccggaccg
      721 ggaacgacac ggtgttcgcg tggtacctga tggggctcac ggcggtctcg ctcgtattgg
      781 ctctctccgt caaggatcgc cgcggccagc cgttgccctg agcgggctca ctgcggcggc
      841 agcaccgagt cgaagtcgaa ctccttgtcc tcttgcgtca cgacgggcgg cgtgtacggc
      901 ttcatgaacc ccgcgtggca cgcgtcgcac gccgcgcgca ggcgctggct cgcttcgcgc
      961 agcggctcgc cgtcctcgct cgtctcgagc gcctccgcca tccgctccgc gttctcggcg
     1021 aacgtctcga aggccgcgaa tcgctcccag atcgcgggca gcgcgagcgt cggaggatcg
     1081 tgcgcgccgg ggtcgaagcg gttcgtcgtc ggcggaaaca gatgcggcac ggcgtgcaac
     1141 atcgccccga tcgttgccgc gttcgcgcgc agcacgtttg cgtcggcagg ctcgccgacg
     1201 gtgaagctgt cgatgggctt catgagattc tcggcttggt ccatcaatgc gcggcgcgct
     1261 tcgatgactt cctcgggctc cgtgaccccg gtccagctcg ggtgcgtgcc cggtacgggc
     1321 gccggcgccg cggatggagc cacattcccc tgcggcgtcg tcgcgggcgg tcccgagcat
     1381 gcggcgacga cggccgcaaa agcccagacg agcgtgctcg gtcgcagcat ggcagcacct
     1441 ccatgtcttc aagctacgat ggtgttcccc aacgtcgcgc gccgcaactg aagcgcgctg
     1501 cggcaacccg caattgacgg gcggcgcgct agaatctcgc catgttcaga tttctgccgc
     1561 cgaccgctct gctcgtcgcg gtgctctgcg ccgccaaaag caacgcacaa gcgcccgagc
     1621 gcgcgacgcg attgccgagc atctcgcccg cgcactgcgt cgcgacactc gaccgccgcg
     1681 agcaggccga tgcgctgcgc tgccccgccg cgctgcgcaa cgcgatcgtc gaagcgcggg
     1741 acgtctgccg cgacgccggc ggcaagctcg ccggcctgcc cgaaggcgac gtatgggcca
     1801 tcgacgtcaa cggcgacggc cgtagcgagc tcgcgttcga tctcgaaagc aacgtgtcgt
     1861 gcaccgatgc gtggagcgtg ttctcgtgcg gcagcctcgg ctgccccagg gcgctctacg
     1921 agctgcgcgc cggcgcgtgg agcgtcgtcg gcggaatctc cgcagcttcg ccggagcagc
     1981 tctcgctcgg cgctgctcgc tcggccgacg gccatcgcgc gctcgaagtg tgctcgcaag
     2041 accgttgtgc cgagcgctgg acgtacgact ggcagggcaa gagctacgaa gtgactcacg
     2101 ccgacgtgcg cggcgcgcgc gtcgagatcg cgggctccgc gcagggtctc catgcgctca
     2161 acgccgcgac gccggtgcgc gcaactccgc gcgcgaacgg cgaagaagtc ggccgctacg
     2221 atgccggaac ggaggtcgta atcatcggca ccgtcgaggg cggcgactgg tattacgtct
     2281 cgccgtgcaa cgcgtgcaag agcggcttcg cgccgaaatc cgccgtcgcc gtcgactgag
     2341 ccgcttcggc gcgtgccgtc agctcgcggg ctcgatgcgc agcacggcgc cgtcgggcgc
     2401 ttccgccgtc agcacgtaca agaggccgtc cgggccttcg cgcacgtcgc ggatgcgctg
     2461 gccgagctcg cgcagcagcg tctcgcgccg gatctcctcc cagcggtcgt tgaactcgat
     2521 gcgctgcagc tgccccgtcc gcggcacctc gccctggcgc aagccgccga cgaacgcgct
     2581 gcgccgccac gccgtgaaca cgtcgccttg atagaacgtc aggccggtca ccgcgatcga
     2641 cggcacccag aagacgatcg gctggatcgt gccctcgagc gtcgggttcg ccgacacgcg
     2701 tggcccgcta tagacccggc cgtagctgac gagcggccag ccgtaattgc cgcccttctg
     2761 cagaatgttg atctcgtcgc cgccgttcgg gccctgctcc gtcgcccaga gctcgccggt
     2821 ccacgggttc aagaccatcg aatgcccgtt gcgatggccg agcgtgtaga tcgcgggcag
     2881 atagcgcgcg tcgttcacga acgggttgtc gggcgggatg ctgccgtcgt cgcgcaggcg
     2941 aatcgttttt ccggcgtagt ccttcggatt ctgcgaccga atgacgtcgg ggccgacgcc
     3001 gggcgcgctg accgtcatgt gcagcatgcc gtccttcgcg aaaccgatgc gcgagccctc
     3061 ggttccggtc gcgccggtcg cgaagatgtc gcgcacatcg acgagcttgt cgttctccca
     3121 gcggccgcgc gcgagcatcg tctcgccggc gtcttcgccg gccggccggt gatacgagaa
     3181 atacacgaag tggttgtccg cgaaacgagg gtgcagcacg acgtccatga gcccctgaag
     3241 cccacgagag cgcaccgctg gaatgccggc gacgggcgcg gggtcgagct tgccgccttt
     3301 gatgagccgc aagcggccgg cgcgctcggt gacgagcatg tcgccgccgg gcaggaacgc
     3361 aatcgaccac ggctgctcga gaccgcgcgc gacgacgacg acgcgcaggt tgcggtgctc
     3421 ggaccgcacc gattcgacga ggaacggccc ggggccgagc ggcggcgagg gccaactcgt
     3481 tccggccgga atgccggcga tggcgcccgg cagcttctgc ggatcgagcg gccagacctg
     3541 cggcggctgg gcgcgcgctg ttgcgattgc gaagacgagc aagctcgccg cggcggcgcg
     3601 gcggacatcg gcgtgacgca tcgagatacc ccctagccgg aacctgagcc ggcgcgcttc
     3661 ggtttaccat acctgccccg cgttgcacac cgcctcgcgg cgattacttg agaggaagcg
     3721 caatgaataa agaactcttc gaaaaaggcc tcgccattcg caagtcggtc atcggcgccg
     3781 agttcgtcga caaggcgctc gcgaccgcgg acgacttcaa catgccgctc caggagctcg
     3841 tgaccgaata ttgctggggc gccgtctggg gccgcgaagg gctcgagaag aaaacgcgca
     3901 gcctgctcaa catcgcgatg ctgagcactc tgaatcgccc gcacgagctc aaggtccacg
     3961 tgaaaggcgc gatccgcaac ggcgcgacga agacggagat ccgcgaggtg ctgctgcagg
     4021 tcgcgatcta tgccggcgtg ccggcggccg tcgactcgtt ccgcgtcgcg aaggaagcgc
     4081 tcgccgagct cgagaagaaa taacgcgcgt tggccgtgcg ctgctcgcgc cgcggcaccc
     4141 gccgccgcgt cgcgcgaccg ctctcgttag cggttacacc ctcggttctg gcatagctct
     4201 tgcaatttcc cttgccgcgt ggcgcttcac cgtggaaata actgcctgaa tcaggaggaa
     4261 taaatgaaag ttctcgtaac cggcggcgcg ggctttacag gcgttcatct cgtacgcgcg
     4321 cttctcgatc gcggcgacga cgtcacggtt ctcgacctga gcgagggtga ctcgctcgat
     4381 accgagccgc tcaaagcgcg cggcgcgaag ttcatcattg gctcggtcgc cgacaaggcg
     4441 gcgctgcgca acgcggtccg tggtcaggaa gtcatctttc atctcgcttc ggccttccgc
     4501 gacatccacc gtggcgcgcc gctgttccaa gaagtggacg tagaaggaac gcggcgacta
     4561 ttgcaagagg caagattggc gggaacgcgt cgcgtggttc actgctcgac gcaaggcgtg
     4621 cacggttcgc tacctgccgg cacggtgccc ggcaacgagg aatcgccgat cgcgcctatc
     4681 gactattact gcgaggcgaa agtggcggcc gaagccgtct gcgacgagtt catcgccgac
     4741 ggcatggaca tcgtcaacgt gcgaccgact tcgatctacg ggcccggcga tacgcacggc
     4801 tggc
//
LOCUS       DASSNN010000345         3657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_192471, whole genome shotgun sequence.
ACCESSION   DASSNN010000345 DASSNN010000000
VERSION     DASSNN010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192471"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFL84_06335"
     CDS             complement(<1..211)
                     /locus_tag="VFL84_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019186179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7608276.1"
                     /translation="MTNHSTMSKPAARRAIAGLTLIELMVALAIGMFLMIGAITVFMQ
                     SRTTFRVNESVARLQENARFVLDTLE"
     gene            complement(228..710)
                     /gene="pilV"
                     /locus_tag="VFL84_06340"
     CDS             complement(228..710)
                     /gene="pilV"
                     /locus_tag="VFL84_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HET7608277.1"
                     /translation="MYSSFKLGPQWDIRRTQSGFSLIEALVSLIVISVGMIGIASLYG
                     QGLRASGTALYRTQAVSLAADMADRIRGNRAATTAYEGVGADRNCDAGDNNDCAPPDM
                     AAHDLWLWQAQVTSQLPGGAGTVRVDTTTPPTYQITVSWQEVGTGATSYTMTVEIPDV
                     "
     gene            complement(725..1438)
                     /locus_tag="VFL84_06345"
     CDS             complement(725..1438)
                     /locus_tag="VFL84_06345"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HET7608278.1"
                     /translation="MNTTRQRGFTLWELLIALLVAGILLSIGVPNVMEFQRNGAMTGA
                     ANDLITAALVARSEAVKRQTFVGWCLSNDPLADPPVCSPGSVVHNSAAGFGYVVWVDE
                     NNNFDANGARILTDPTDGNAVVDADEEVLRRTVAPSGNIGLSASCGYASFGPTGALRQ
                     VGAACTPAAPAPATIRVVLFCDDRGRRAASGALSSARVVRIDRPGRAQVITETEGVNT
                     MIGGLAGGPGMGAVNTACP"
     gene            complement(1532..2068)
                     /locus_tag="VFL84_06350"
     CDS             complement(1532..2068)
                     /locus_tag="VFL84_06350"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HET7608279.1"
                     /translation="MRAQHAFTLWELLCTLSIAAVTLTLGVPAFRGFLLDARLTADVN
                     GWVLAVQLARSEAAKRGRPVIVCKTDDTRRCGDKGLPIDAGWMVYVNLDDEYPPQRSP
                     AEPLLYMHAAELTGTVVSNRPYFEFRVGRRSTNGTVVFCDSRGAAAARAVIISYTGRP
                     RVARVDADGRPLQCAALT"
     gene            2230..2640
                     /locus_tag="VFL84_06355"
     CDS             2230..2640
                     /locus_tag="VFL84_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018948367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET7608280.1"
                     /translation="MTRDEATALLAERGVLPTAQRIDIALLTLARPQHLSAEQIIAAI
                     RANGLKISKATVYNTLNLFCEQGLLRTVDVDPTRQFYDSSTSAHHHFYNVDTGELTDI
                     PPESVSLQVTTALPAGTEQAGVDVVVRVRARRGG"
     gene            2717..2792
                     /locus_tag="VFL84_06360"
     tRNA            2717..2792
                     /locus_tag="VFL84_06360"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2750..2752,aa:Thr,seq:cgt)
     gene            complement(3469..>3657)
                     /locus_tag="VFL84_06365"
     CDS             complement(3469..>3657)
                     /locus_tag="VFL84_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608281.1"
                     /translation="ERLVPAVAGRERLTQAHEPVEGSLGLGDVAVATMTMLVVLLVVA
                     VTALVVRTTTLVAGRVAR"
BASE COUNT          596 a         1235 c         1211 g          615 t
ORIGIN      
        1 gctcgagcgt gtcgagaacg aagcgcgcgt tctcctgcag ccgcgcgacg gactcgttga
       61 cgcggaaggt cgtgcggctc tgcatgaaca ccgttatcgc gccgatcatg aggaacatgc
      121 cgatcgcgag ggcgaccatg agctcgatca gcgtcaggcc ggcgatagcg cggcgcgctg
      181 cgggcttcga catcgtggag tggttcgtca tcgtagttcg ctcggcgtca aacgtccggt
      241 atctcgaccg tcatcgtgta gctcgtcgct cccgtgccga cctcttgcca cgacacggtg
      301 atctgataag ttggcggcgt cgtcgtgtcg accctgacgg tgcccgcgcc gcccggaagc
      361 tggctcgtaa cctgcgcttg ccagagccac agatcgtgcg cggccatgtc gggcggcgcg
      421 caatcgttgt tgtcgccggc gtcgcagttg cggtccgccc ctacgccctc gtacgccgtc
      481 gtcgcggcgc gatttccgcg gattcgatcg gccatgtcgg cggcgaggct gacggcttgc
      541 gtgcggtaca gcgccgtgcc gcttgcccga aggccctggc cgtagagcga tgcgatgccg
      601 atcatcccga cggagatgac gatcaacgac accaaggctt cgatcaagct gaagcccgac
      661 tgcgttcgac ggatatccca ctgcggcccg agtttgaagc tgctgtacat cgctgctgct
      721 ctctttatgg gcacgccgtg ttgacggcgc ccatgcccgg cccgcccgcg aggccgccga
      781 tcatggtatt gacgccttcg gtttccgtaa tcacttgagc acggcccggg cgatcgattc
      841 ggacgacgcg cgcgctcgac agagcgccgc tcgcggcgcg ccggccgcgg tcgtcgcaaa
      901 acagcacgac gcggatcgtc gccggtgccg gcgccgccgg cgtgcaggcc gcaccgacct
      961 ggcgaagcgc gccggtcggg ccgaacgacg catagccgca gctcgcggag aggccgatat
     1021 tgccgctcgg ggcgacagtc cgcctgagca cctcttcgtc ggcgtcgacg acggcgttgc
     1081 cgtcggtcgg gtcggtcagg attcgagcgc cgttcgcgtc gaagttgttg ttctcgtcga
     1141 cccacacgac gtagccgaag ccggcggcgg agttatggac gacgctgccc ggcgagcaga
     1201 cgggcgggtc ggccagagga tcgttgctca agcaccagcc gacgaaggtt tggcgcttga
     1261 ccgcctccga gcgtgccacg agcgcggccg tgatgagatc gttggcggct cccgtcatcg
     1321 cgccgttccg ctgaaactcc atgacgttcg ggacgccgat ggacaacagg atgccggcga
     1381 cgagcagggc tatcagtagc tcccacagag tgaatccgcg ctgccgagtc gtgttcatgg
     1441 cggcggacta tacggagcgg ccgcgcgcgc gcagcggttc gcccgacgac cggcgtcggc
     1501 gtgacgacga gcggcggttc cggccgcata gttatgtcaa cgctgcgcac tggagcgggc
     1561 gcccgtccgc gtcgacgcgg gcgactcgcg gccgccccgt gtagctgatg atgacggcgc
     1621 gtgcagcggc ggcgccccgg ctgtcgcaga acacgacggt gccgttggtg cttcgcctgc
     1681 ccacgcgaaa ttcgaaatac gggcggttac tgaccacggt gcccgtgagc tcggcggcat
     1741 gcatgtagag gaggggctcc gccggcgagc gctgcggcgg gtattcgtcg tcgagattca
     1801 cgtagaccat ccaccctgcg tcgatcggca agcccttgtc gccgcaacgc cgcgtatcgt
     1861 cggtcttgca gacgatgacc ggccggccgc gcttcgccgc ctcgctgcgc gcgagctgca
     1921 ccgcgagcac ccacccgttg acgtcggccg ttagccgggc gtcgagcaga aagccgcgaa
     1981 acgcggggac gccgagtgtc agcgtgacgg ccgcgatcga gagcgtacag agcagctccc
     2041 agagcgtgaa cgcgtgttgt gcgcgcatgg gaccttctcc cccggcggtg gtggaccaca
     2101 ttacggagcg gcctctcgtg gatcgagcgt tcaatcggcc gagatcgagc cgaaatcgag
     2161 attgcttttg atatcgatgc cggctcgtgg gtatactggc caagtcgctg atttatttgg
     2221 tatcgagcta tgacacgcga tgaagcaaca gcgttgctcg ccgagcgagg cgtgttgcct
     2281 actgcccagc gaatcgatat cgcgctgctg acgttggcgc gtccgcagca cctgtcggcc
     2341 gagcagatca tcgccgcgat tcgcgcgaac gggctcaaga tctcgaaggc gaccgtctac
     2401 aacacgctga acttgttctg cgagcagggt ctgttgcgaa ccgtcgacgt cgacccgacg
     2461 cggcagttct acgattcgtc gacgagcgcg caccaccact tctacaacgt cgacacgggc
     2521 gagctcacgg acatcccgcc cgagtcggtc tcgctgcaag tcacgacggc gctacccgcg
     2581 ggaacggagc aggccggcgt cgacgtcgtc gtgcgcgtcc gcgcccgtcg cggcggctga
     2641 cgcgcgaccg gtccttctca tttaattatc ttgcatgggc tccgtgccgg ccggcacaat
     2701 acgcgccttc catcctgccg gtgtagctca gttggtagag catccgcttc gtaagcgggg
     2761 ggtcgctggt tcaaatccgg ccaccggcac caaaccccac agagcaatgc gggcttcgac
     2821 aagcccgcga tcggcttagc tccgctcgac gccgcgtcgc gcgctcgagc tcgccgtcac
     2881 gtcggcgact gccgctttga agcgtccaca tttaggcaaa tcgcgcgaag tcacgtgcgg
     2941 aattcgcatt tgacagggac cttatgtgac tgccgtctcc gtatgaggct ccgttcgtcg
     3001 gtgtaatgcg acacgcaaga acgcgcgacg tgcagggaag cgacccactc gcgccggaag
     3061 ttgcaccgat ttcagggaag tagttcaggg agaacttcgg tgaccacgaa actgtacggg
     3121 ctcgttgcgt tagccgtcgg ggcagtcctg ctcggtccgg ctcgtgctca gacagccgag
     3181 gatctgtggc agtcctgtat ggaaacctgg ggcgacgaag ccatttgcgg accgcggccg
     3241 gaaccgccgc cgcctcctcc gcaggaagac ccgccgcccc cgccgtcgga ggacccgcct
     3301 ccgccgcccg aggagccgcc gcctccgccg ccgccggagg agccgccgcc tccgcctccg
     3361 ccgccggagc agccgccgcc tccgcctccg ccgccgccgc cgcctcctcc tgcgccggag
     3421 cagccgccgc agtgcgcgag cggtaaccac accggccacg ccgtcgcctc atcgggcaac
     3481 gcgaccggcc acaagtgtgg tggtgcgaac aaccagcgcg gtgaccgcca cgacaagaag
     3541 caccacaagc atggtcatcg tggccacggc cacgtcgcca aggccaaggc tgccttcgac
     3601 cggttcgtga gcttgtgtaa gtctttcgcg gccagccacg gccggcacta gtctctc
//
LOCUS       DASSNN010000346         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_297481, whole genome shotgun sequence.
ACCESSION   DASSNN010000346 DASSNN010000000
VERSION     DASSNN010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297481"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1775)
                     /locus_tag="VFL84_06370"
     CDS             complement(<1..1775)
                     /locus_tag="VFL84_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019961772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HET7608282.1"
                     /translation="MTESHRIIAAALLCAALAACTPPSTSTSNPSAPGPTTADALAFL
                     DEVERQFTTLGEEAARIGWVNETYINFDTDWLSANIGARITELGVKYAKQATDFDDVD
                     VPVDARRKLELLKLGLTLPAPEKPGAAQELAEITTRLGSTYSTGKIEFEGKTVPQGDT
                     EELMRRLRDPNKLEEVWTKWHDYAKGMKADYVREVEIANEGARELGFADVGALWRSGY
                     DMPPDAFAAEVDRLWNQVKPLYDDLHCYVRGKLNEKYGDDIVPLDHPIRADLLGNMWA
                     QSWGELYDFIAPADADTGIDLNRLLVEKDYDAIKLVKTGENFFSSLGFDPLPETFWER
                     SQIVRPEGREVVCHASAWDLDAKDDLRIKMCAVVGAEDFRTVHHELGHNYYQRAYKDQ
                     PTLFRGGANDGFHEAIGDTITLSITPEYLRQIGLIDTVPDASKDIGLLLQQALDGVSF
                     LPFGLLIDKWRWQVFSGETTPDEYNTDWWALRTKYQGVRPPNERPADAFDPGAKYHIP
                     GNTPYMRYFLARILQYQFYKAACDISGWDGPLHRCSFYGSKEVGQRLNAMLAMGQSQP
                     WQEALQAFTGERQMDGSAILAYYAPL"
     gene            1842..>2046
                     /locus_tag="VFL84_06375"
     CDS             1842..>2046
                     /locus_tag="VFL84_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET7608283.1"
                     /translation="MKIHVLTTGGTIDKVYFDAKSEFEVGPPMIAELLKEAHVTAEVT
                     IEPVLAKDSLELSDADRRLIRERV"
BASE COUNT          327 a          638 c          681 g          400 t
ORIGIN      
        1 agcggcgcgt agtacgcgag gatcgccgag ccgtccatct ggcgttcgcc cgtgaacgct
       61 tgcaatgcct cctgccacgg ctgcgattgg cccatcgcga gcatcgcgtt cagcctttgc
      121 ccgacttcct tcgagccgta gaacgagcag cgatgcagcg gcccgtccca gcccgagatg
      181 tcgcaagcgg ctttgtagaa ctggtattgc aggatgcgcg cgaggaagta gcgcatgtac
      241 ggcgtgttgc cgggaatgtg gtacttcgcg cccggatcga acgcgtcggc gggccgctcg
      301 ttcggcggcc gcacgccttg gtacttcgtc cgcagcgccc accagtccgt gttgtactcg
      361 tccggcgtcg tctcgcccga gaacacctgc cagcgccact tgtcgatgag gagcccaaag
      421 ggcaggaacg agacgccgtc gagcgcctgt tgcagcagca agccgatgtc cttcgacgcg
      481 tccggcacgg tgtcgatgag cccgatctgg cgcaagtact cgggcgtgat cgacagcgtg
      541 atcgtgtcgc cgatggcttc gtggaagccg tcgttcgcgc cgccgcggaa caaggtcggc
      601 tgatccttgt acgcgcgctg atagtagttg tgcccgagct cgtgatgaac cgtgcggaaa
      661 tcctccgcgc ccacgacggc gcacatcttg atgcgcagat cgtccttcgc gtcgaggtcc
      721 caggccgacg cgtggcagac gacctcgcgg ccttcgggcc gcacgatctg cgagcgttcc
      781 cagaacgttt cgggcagcgg atcgaagccg agcgacgaga agaaattctc gccggtcttc
      841 acgagcttga tcgcgtcata atctttctcg acgagcaggc ggttcaagtc gatgccggtg
      901 tcggcatcgg cgggcgcgat gaagtcgtag agctcgcccc agctttgcgc ccacatgttg
      961 ccgagcaggt cggcgcggat cggatgatcg agcggcacga tgtcgtcgcc gtacttctcg
     1021 ttcagcttgc cgcgcacgta gcagtgcagg tcgtcgtaga gaggctttac ttgattccag
     1081 aggcggtcca cctcggcggc gaacgcgtcc ggcggcatgt cgtagcccga gcgccaaagc
     1141 gcgccgacgt cggcgaagcc gagctcgcgc gcgccttcgt tggcgatctc gacctcgcgc
     1201 acgtaatccg ccttcatgcc tttcgcgtag tcgtgccact tcgtccagac ttcttcgagc
     1261 ttgttcggat cgcgaagccg gcgcatcagc tcttcggtgt cgccttgcgg caccgttttg
     1321 ccttcgaact cgatcttgcc ggtgctgtag gtgctgccga gacgtgtcgt gatttccgct
     1381 agctcctgcg cggcgccggg cttttcgggt gccggcagcg tgaggccgag cttcaagagc
     1441 tcgagcttgc gccgcgcgtc gacgggcacg tcgacgtcgt cgaaatcggt cgcttgcttc
     1501 gcgtatttga cgccgagctc ggtgatgcgc gcgccgatgt tggcggagag ccagtcggtg
     1561 tcgaagttga tgtaggtctc gttgacccag ccgatgcgcg ccgcttcctc gccgagcgtc
     1621 gtgaactgcc gctcgacctc gtccaagaaa gcgagcgcgt ccgcggtcgt cggtccgggc
     1681 gccgacgggt tcgacgtgga cgtggacggc ggcgtgcaag ccgccagcgc ggcgcacagc
     1741 agcgcggcgg cgatgatgcg atgtgattcc gtcatgatgg tccttgcgcc tttctcgtat
     1801 gccggtagcg tatagcgtag cgcgctccgt accataccgc gatgaagatt cacgtcctaa
     1861 cgaccggcgg caccatcgac aaggtgtatt tcgacgcgaa gagcgagttc gaggtcggtc
     1921 cgccgatgat cgcggagttg ctgaaagaag ctcacgtgac ggctgaggtt acgatcgagc
     1981 cggtgctcgc gaaggacagc ctcgagctca gcgatgcaga tcggcggctg attcgcgagc
     2041 gcgtcg
//
LOCUS       DASSNN010000347         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1075692, whole genome shotgun sequence.
ACCESSION   DASSNN010000347 DASSNN010000000
VERSION     DASSNN010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075692"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..745
                     /locus_tag="VFL84_06380"
     CDS             128..745
                     /locus_tag="VFL84_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608284.1"
                     /translation="MSHLRKVDHITYACAKGTIEKWAWFHIDVEGGTLINRIDDVQPE
                     NPDSSMKIWCIDYGDFGIALVEGIDRRKQSQVTRFVERHGDHSCQHVAYDCHNLDSFR
                     KHLLANGGHPRGAVLTRHDGFGLLKQLFAKGYAPGDPTEASFAEYVERPRGDDESLRI
                     TFSQGAGRGFYEQIEDAFAKGDDEPFIDFSKMPKNWRVPPPARRG"
     gene            complement(839..>2417)
                     /locus_tag="VFL84_06385"
     CDS             complement(839..>2417)
                     /locus_tag="VFL84_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7608285.1"
                     /translation="NDVFAVSLDTGEILWQYTANLPDDLPSVCCGWNNRGVAISEDRV
                     FMGRLDAKLVALDRKTGWVYILDRTNGTPLVGIDENNVFMGRLDGKLVALDRKSGAPV
                     WTIQAESPKENFSITPAPVYYDGLVITGFAGADRGTRGRVKAYDARDGRLVWTFYTIP
                     GPGEPGHETWPQDNDAWKYGGAAVWQTPAVDPELGLVYFSTGNAGPDYNGTVRAGDNL
                     YSVSIVAIEGKTGKYRWHFQQVHHDIWDYDSSNPVVLMDLQVNGRARKALVEVSKTGW
                     AYILDRVTGEPIVGIDERPVPQEPRQATAATQPFPRGDAVVPQHVDIAPEGIPLVNEG
                     RIFTPFLGDDPTVVMPGIWGGASWPPSAYDPRAQRLFVCASSVVNGYTGGGDANFPAP
                     VEGGRYSGGTPMTPRVPRTGIIAAMDMTTNTVVWRYRWQEQCYSGTLATAGNLLFVGR
                     NDGRLTALDSRTGRQLWEFQTGAGMHAPTSTFMHGGKQYVLAYSAGNALIGSARGDSV
                     WLFALDGAKGDGSIFVR"
BASE COUNT          411 a          808 c          793 g          405 t
ORIGIN      
        1 gaccggccgc atctgacgcg cgcgcaacgc gtgctcgcgc tggccgccgc ggccggcgca
       61 cccggcgcgc cggtggacaa cgccgacgga aacgactatc caataggttg aaagcatgga
      121 gcgagtcatg tcgcacctca ggaaagtcga tcacatcact tacgcttgcg cgaaaggcac
      181 gatcgagaag tgggcgtggt tccacatcga cgtcgaaggc ggcacgctga tcaatcgcat
      241 cgacgacgtg cagccggaga atcccgacag cagcatgaag atctggtgca tcgactatgg
      301 cgacttcggc atcgcgctcg tcgaaggcat cgatcgacgc aaacaatcgc aagtgactcg
      361 cttcgtcgag cgccacggcg atcactcgtg tcagcacgtg gcgtacgact gtcacaacct
      421 cgacagcttc cgcaagcatc tgctcgcgaa cggcggccac ccgcgcggcg ccgtgctgac
      481 gcgccacgac ggtttcggcc tgttgaagca gctgttcgcg aaaggctacg cgcccggcga
      541 tccgaccgaa gcgagcttcg ccgaatacgt cgagcggccg cgcggcgacg acgagagcct
      601 gcgcatcacg ttctcgcaag gcgcgggccg cggcttctac gagcagatcg aggacgcctt
      661 cgcgaaaggc gacgacgagc ccttcatcga cttctcgaag atgccgaaga actggcgcgt
      721 gccgcccccc gcccgccgcg gctagcgcaa aaggggacag atctattttc gtccgcgcgt
      781 atccgcaaaa gggacagacg cacttaacgg acgaaaatag atctgtcccc ttttgcgctt
      841 aacggacgaa aatagatccg tccccttttg cgccgtcgag ggcgaagagc cagacgctgt
      901 cgccgcgggc cgagccgatc agcgcgttgc cggccgagta ggcgagcacg tattgcttgc
      961 cgccgtgcat gaacgtgctc gtcggcgcgt gcatgccggc gcccgtttgg aattcccaga
     1021 gctgccggcc cgtgcgcgag tcgagcgccg tgagcctgcc gtcgttgcgg ccgacgaata
     1081 gcaagttgcc ggccgtcgcg agcgtgccgc tgtagcactg ctcctgccag cgatagcgcc
     1141 agacgacggt gttcgtcgtc atgtccatcg cggcgatgat gcccgtgcgc ggcacgcgcg
     1201 gcgtcatcgg cgtgccgccc gaatagcggc cgccttcgac gggcgcgggg aaattcgcgt
     1261 cgccgccgcc cgtgtagccg ttcacgaccg acgatgcgca gacgaagagc cgctgtgctc
     1321 gcgggtcgta cgcgctcggc ggccagctcg ctcctcccca gatgccgggc atgacgacgg
     1381 tcgggtcgtc gccgagaaac ggcgtgaaga tgcggccttc gttgacgagc ggaattcctt
     1441 cgggcgcgat gtcgacgtgc tgcgggacga ccgcgtcgcc gcgcgggaac ggttgcgtcg
     1501 cggccgtcgc ttgccgcggc tcctgcggca cgggccgctc gtcgatgccg acgatcggct
     1561 cgccggtcac gcggtcgaga atgtacgccc agcccgtctt gctgacctcg acgagcgctt
     1621 tgcgcgctcg cccgttcact tgcaggtcca tgagcacgac gggattgctc gagtcgtaat
     1681 cccagatgtc gtgatgcact tgctggaaat gccaccggta cttaccggtc ttcccctcga
     1741 tggctacgat cgacaccgag tacaagttgt cgccggcgcg caccgtgccg ttgtagtcgg
     1801 gccccgcgtt ccccgtcgag aagtagacga gcccgagctc gggatcgacg gccggcgtct
     1861 gccacacggc cgcgccgccg tatttccacg cgtcgttgtc ctgcggccag gtctcgtggc
     1921 ccggctcgcc ggggcccggg atcgtataga acgtccagac gagccggccg tcgcgcgcgt
     1981 cgtacgcttt gacgcggccg cgcgtgccgc gatcggcgcc cgcgaacccc gtgatgacga
     2041 ggccgtcgta gtagaccggc gccggcgtga tcgagaagtt ctccttcggc gactccgctt
     2101 gaatcgtcca gaccggcgcg ccgctcttgc ggtcgagcgc gacgagcttg ccgtcgagcc
     2161 tgcccatgaa gacgttgttc tcgtcgatgc cgacgagcgg cgtgccgttc gtgcgatcga
     2221 gaatgtagac ccagcccgtc ttgcgatcga gcgcgacgag cttggcgtcg aggcggccca
     2281 tgaacactct gtcttcgctg attgcgacgc cgcggttgtt ccagccgcaa cagacggacg
     2341 gcagatcgtc gggcagattc gccgtgtact gccaaaggat ctcgcccgta tcgagcgaca
     2401 ccgcgaacac gtcgttc
//
LOCUS       DASSNN010000348         3348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_567226, whole genome shotgun sequence.
ACCESSION   DASSNN010000348 DASSNN010000000
VERSION     DASSNN010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567226"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFL84_06390"
     CDS             <1..187
                     /locus_tag="VFL84_06390"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HET7608286.1"
                     /translation="LLDFFGRCAARLTDAEIAAFERVLELPDPTLHSYLLGRSAPDDA
                     ATAELIERIRASVGAER"
     gene            174..557
                     /locus_tag="VFL84_06395"
     CDS             174..557
                     /locus_tag="VFL84_06395"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HET7608287.1"
                     /translation="MLNVERSRVLCLWWVMLHVLIGAAFALVGWPVVWRGIAIVAVVV
                     HAVVRRPPPSPSTVIVAEGGTCAIPEWNTGMRPLGARTLICPFWIRLDLEAGPWRRDI
                     LLVVDQVRPEEWRQLRALLNRARCE"
     gene            682..1302
                     /gene="rpoE"
                     /locus_tag="VFL84_06400"
     CDS             682..1302
                     /gene="rpoE"
                     /locus_tag="VFL84_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HET7608288.1"
                     /translation="MGSTEQRLTEQAERHPPGSGAADLALIRRVQQGDRSAFDLLVVK
                     YQHKIVKLIMRYVRDSSEALDVAQEAFLKAYRAAPSFRGDSAFYTWLYRIAINTAKNH
                     LVATGRKPVSYDLDTQDPEQAGILNELRELDTPEALVQSEEIRDTINRAIHALPEELR
                     TAILLREIEGLSYEEIAQTMECPVGTVRSRIFRAREAIDKALAPLL"
     gene            1326..1946
                     /locus_tag="VFL84_06405"
     CDS             1326..1946
                     /locus_tag="VFL84_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HET7608289.1"
                     /translation="MTDQINDQISAFIDNELTADESALLVRRFERDSEARARAMRYTL
                     IGASLRGELLEPHPSVLRQRIAAALSGNAVPSVPKAREPWSDKWARPLIGLGIAATVA
                     AVAIGTLRSLNEASLPGAALPAGPSVVQVTDRDPAYVVPQPNATSGVGGIRLTNYLMH
                     HGEFASGLTRTSVNSNVLGGAMEPPPTVASEGAVSPAKPVVQQGAE"
     gene            1951..2913
                     /locus_tag="VFL84_06410"
     CDS             1951..2913
                     /locus_tag="VFL84_06410"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HET7608290.1"
                     /translation="MRNALPVAVPSLLLFALLGGQASAQQARDWLERMNSAVEDLNYR
                     GTFVHVLDGTPETLHIVHRSAGGQSGERILSLDGARREIVREGARVQGIFPDRRLVLL
                     ETRRDVSPLATALPSDTSQLAPHYEIELGGSERVADRAVQALEIKPRDEFRYGYMLWL
                     DQETAMPLKSHLVDEQGNVVEEILFTYIEFPADIPAADLEPTIDTTGFTTQRAADAKP
                     LDASIPWRAAVLPGGFKLSIATESRIAGSDTPVGHLVYSDGLATVSVFIEDPATEAEV
                     GEGFSTMGSTNVYSLTLSSGQKATAIGEVPRQTVRTIASSLVAQ"
     gene            2959..>3348
                     /locus_tag="VFL84_06415"
     CDS             2959..>3348
                     /locus_tag="VFL84_06415"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HET7608291.1"
                     /translation="MVRQSTAGEVTVELRPPPRCAACAGACLWYRVSPSEQLTLAANG
                     TIPVGSTVAVTLPDRYVLAAAALVYGWPLGALLAGGATAAAIFGTDLAAALGAAGALA
                     AALLAASPLRRRLERATVRRLAVRCAPE"
BASE COUNT          535 a         1116 c         1120 g          577 t
ORIGIN      
        1 ccttctagac ttcttcgggc gctgcgcggc gaggctcacg gacgcggaaa tcgcggcgtt
       61 cgagcgggtt ttggagctgc cggatccgac gctgcactcg tatcttttgg ggcgcagtgc
      121 tccagacgac gccgccaccg cagagctcat cgagcgaata cgcgcgagcg ttggtgctga
      181 acgttgagcg atcgcgcgtt ctctgtctgt ggtgggtgat gctgcacgtg ctgatcggcg
      241 cggcgttcgc gctcgtcggg tggccggtgg tgtggagggg gatcgcgatc gttgcggtcg
      301 tcgtgcacgc cgtcgttcgc cgtccgccgc cgtcgccgag caccgtgatc gtggccgagg
      361 gcggaacttg tgccattccc gaatggaata cgggcatgcg gccgctcggc gcacggacct
      421 tgatctgccc gttctggatc agattggacc ttgaagccgg tccatggcga cgcgacatat
      481 tgctcgtcgt ggatcaggtt cggcccgaag aatggcggca attgcgagcg cttctcaatc
      541 gggcgcgttg cgaatgagcg acgcgtgtca cgcgatgcct gaggccgctc ggggtcgatc
      601 tgcgctagaa tcattccacg atcgtgagcc gcctcgcgcg ggcgaaaacc ttcgcggtgt
      661 cgagcgaact tttggtgcag catggggtct accgagcagc gtttgacaga gcaggccgaa
      721 agacaccctc ccggtagcgg agccgctgac ctagccctga tccgacgcgt gcagcagggc
      781 gacaggtcgg cgttcgatct gttggtcgtc aagtaccagc acaagatcgt caagctcatc
      841 atgcgatatg ttcgtgactc ttccgaggct cttgacgtag cgcaggaagc tttcttgaag
      901 gcgtaccgtg ctgctccgtc gtttcgcggt gatagcgctt tctatacctg gctctatcga
      961 atcgcgatca atactgcgaa gaaccatctc gtcgcgacgg ggcgcaagcc cgtcagctac
     1021 gacctcgaca cgcaagaccc ggagcaagcc ggcatcttga acgagctgcg cgagctcgac
     1081 actcccgagg ctctcgtcca gtccgaggag attcgcgaca cgatcaatcg ggccattcac
     1141 gcgctgccgg aagagctccg caccgcaatt ctgcttcgtg aaatcgaagg cttgagctat
     1201 gaagagatcg cgcaaaccat ggaatgcccg gtgggcaccg tccggtcgcg gatctttcgg
     1261 gctcgcgaag cgatcgacaa ggcccttgcg ccgttgttgt aagccaaacg agccactcgc
     1321 cgaacatgac agaccaaatc aacgaccaaa tctccgcctt catcgacaac gagcttacgg
     1381 ccgacgaaag cgcgctgctc gtgcggcgct tcgagcgcga ctcggaagcg cgcgctcgtg
     1441 cgatgcgcta cacgttgatc ggcgcgtcgc ttcgcggcga attgctcgag ccccatccgt
     1501 ccgtgctgcg gcagcgcatt gccgcggcgc tgtccggcaa cgccgtgccg agcgtgccga
     1561 aggctcgcga gccgtggagc gacaagtggg cccgcccgct gatcggtctc ggcatcgcgg
     1621 ccacggtcgc cgccgtcgcc atcgggacgc tgcgctcgct gaacgaagcg tcgctgcctg
     1681 gcgccgccct gccggcgggt ccaagcgtag tgcaggtcac cgatcgcgat ccggcctacg
     1741 tcgtgccgca acccaatgcc acgtcgggag tcggcggcat tcggctcacg aactacctga
     1801 tgcaccacgg cgagttcgcg tcgggcctga cgaggacgtc ggtcaactcg aacgtcctgg
     1861 gcggcgcgat ggagcccccg cccacagtcg cgagcgaggg cgcggtctct ccggcgaagc
     1921 cggtcgtcca gcaaggggcg gagtagacgg atgcggaacg cactgcccgt cgccgttccg
     1981 tcgttactat tgttcgcatt gctcggcggc caggcgtcgg cgcagcaagc gcgcgactgg
     2041 ctcgagcgca tgaacagcgc cgtcgaagat ctgaactacc gcggcacctt cgtgcacgtg
     2101 ctcgacggca cgccggagac gctgcacatc gtgcatcgca gcgccggcgg tcagagcggc
     2161 gagcgaattc tctcgctcga cggagcgcgc cgcgagatcg ttcgggaggg cgcgcgcgtt
     2221 caaggaatct ttccggatcg gcggctcgtg ttgctcgaga cgcgccgcga cgtgagcccg
     2281 ctcgcgaccg ctttgccgag cgacacgtcg cagctcgcgc cgcactacga gatcgagctc
     2341 ggcggcagcg agcgcgtcgc cgatcgtgcc gtgcaagctc tcgagatcaa gccgcgcgac
     2401 gagttccgct acggctacat gctgtggctc gatcaagaga ccgcgatgcc gctgaaatcg
     2461 cacctcgtcg acgagcaggg caacgtcgtc gaggagatcc tgttcacgta catcgagttc
     2521 ccggccgaca ttcccgccgc ggatctcgag ccgaccatcg acacgacggg cttcacgacg
     2581 cagcgcgcgg ctgacgccaa gccgctcgac gcgtcgattc cctggcgcgc cgcggtcctc
     2641 ccgggcggct tcaagctgtc gatcgcgacg gagagccgca tcgcgggctc cgatacgccg
     2701 gtcgggcacc tcgtctattc ggacggcctc gccaccgtat cggtcttcat cgaggatccc
     2761 gcgacggaag cggaagtcgg cgagggtttc tcgacgatgg gcagcacgaa cgtctactcg
     2821 ctgacgctga gcagcgggca gaaagccacg gcgatcggcg aagtgccgcg gcaaacggtg
     2881 cgcacgatcg cctcgtcact cgtcgcgcag tagcttgagg agcgttccat ttcccttcgc
     2941 gattgcaccg cggtcggcgt ggtccgtcag tcgacggccg gcgaagtgac cgtcgagctc
     3001 cggccgccgc cgcgctgcgc ggcgtgtgcc ggtgcctgcc tctggtaccg agtctcgccg
     3061 agcgagcagc tgacgctcgc cgcgaacggc acgattcccg tgggctcgac cgtcgccgtg
     3121 acgttgccgg atcggtacgt cctcgccgcc gccgcgctcg tctacggctg gccgctcggc
     3181 gcgctgctcg cgggcggcgc gaccgccgcg gctatcttcg gcaccgacct cgccgctgcg
     3241 ctcggcgccg cgggggctct ggccgcagcg ctgctcgcgg cgtcgccgct gcgccggcgc
     3301 ctcgagcgcg cgaccgtgcg ccgcctcgcc gtgcgttgcg cgcctgaa
//
LOCUS       DASSNN010000349         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_193107, whole genome shotgun sequence.
ACCESSION   DASSNN010000349 DASSNN010000000
VERSION     DASSNN010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193107"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VFL84_06420"
     CDS             complement(<1..592)
                     /locus_tag="VFL84_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012381722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HET7608292.1"
                     /translation="MTRGWEFWIDRGGTFTDVVARAPSGELKTLKLLSDDPGRYEDAA
                     VAGIARLLGAVPADERRVTAVKMGTTVATNALLERRGEPTVLVITAGFEDAIRIGGQQ
                     RPEIFALDIRLPEMLYTRVVGAAERVDAHGRVLEPLDTAKLERDLTAARAAGLDSVAV
                     ALLHAVRFPRHEAEAAEVARSLGFRQVSVSHRVLPLP"
     gene            725..1174
                     /locus_tag="VFL84_06425"
     CDS             725..1174
                     /locus_tag="VFL84_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET7608293.1"
                     /translation="MKRRIQDGFSLIELVVVITILGILAAFAVPRFASLEGQARLAST
                     QALAGSVRSGAALAHALWLAQGDPNSTSVTMEGEPIAMAFGYPDAAAIEGTLVEYTGF
                     ELTSAGGAAVFTKKSQSGETIANCFVTYTPAPAANQAPQIVATTGGC"
     gene            complement(1233..>2042)
                     /locus_tag="VFL84_06430"
     CDS             complement(1233..>2042)
                     /locus_tag="VFL84_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7608294.1"
                     /translation="DNLMLVGAATLLLAIPMILWIQRLKVVDLHNESLQANLGAAQMG
                     GKWWQGFHDFAKSPYLLAIGLFILIYTAIQAFIYFQQTDLLRIYAEREERTTILAIRD
                     AVVNLLTFGLGLFVTNRIVKRLGMPVTLALLPVFCAVTFLILAMAPMLAVLLAVDIAR
                     RAGEFGITRPAREMLFTRVDRETRFKTKPVVDVVVYRGGDAWWGVIFAFLSDSVGFAF
                     AAMSLIGAAFAAVWVASGVYLGRAFHRRETGVETAESGRPARREVVAEQRT"
BASE COUNT          365 a          708 c          671 g          298 t
ORIGIN      
        1 tcggcaacgg caggacgcga tgcgacacgg atacttgcct gaatccaagc gagcgcgcga
       61 cttccgcggc ctcggcttcg tgccgcggaa agcgaaccgc atgcagcagc gcgaccgcga
      121 cgctgtcgag cccggccgcg cgggccgccg tgaggtcgcg ctcgagcttc gcggtgtcga
      181 gaggctcgag cacgcggccg tgcgcgtcga cgcgctcggc ggcaccgacg acgcgcgtat
      241 agagcatctc gggcagccga atgtcgagcg cgaagatctc gggccgctgc tggccgccga
      301 tccggatcgc gtcctcgaac cccgccgtga tgacgagcac ggtcggctcg ccgcgccttt
      361 cgagcagggc attcgtcgcc acggtggtgc ccatcttgac ggccgtcaca cggcgctcgt
      421 ccgcgggcac ggcccccagc agccgcgcga tgccggcgac cgcggcgtcc tcatagcgcc
      481 ccggatcgtc ggacagcagc ttcagcgtct tgagctcgcc ggacggcgcc cgcgcgacga
      541 cgtcggtgaa cgtgccgccg cggtcgatcc agaactccca accccgcgtc atgaacggcg
      601 agattacaaa acgcaggaga gcgtcgtcac ggtccgtcgg cgatgtggcg ccgatcacag
      661 cccggtccgg cggcgtctcg tagagtcagc atattccaag cggcattcga gctacggggg
      721 agcggtgaag agaaggattc aggacgggtt ctcgctgatc gagctcgtcg tcgtcatcac
      781 gattctcggc atccttgcgg cattcgccgt gccccgcttt gcatcgctcg aaggccaagc
      841 ccgcctcgcg tcgactcagg cgctcgccgg cagcgttcgc tcgggcgccg cgctcgcgca
      901 cgcgctgtgg ctcgcgcagg gcgatcccaa cagcacgagc gtgacgatgg aaggcgagcc
      961 gatcgcaatg gcgttcggct atccggacgc cgcggcgatc gaaggcacgc tcgtcgagta
     1021 caccggcttc gagctcacgt cggccggcgg cgccgccgtt ttcacgaaaa agtcgcagtc
     1081 cggcgagacg atcgcgaact gcttcgtcac gtacacgccc gcgccggccg caaaccaggc
     1141 cccgcagatc gtcgcgacga cgggcggctg ctagcggccc tcgtcgttcg aacggtccga
     1201 agaagagggc gcctacggcg ccctcgccgt ttctaagtcc tctgctcggc gacgacctcg
     1261 cggcgcgcgg gccgtccgga ctccgccgtc tcgacgcccg tctctcggcg gtgaaaagcg
     1321 cggccgagat agacacccga cgcgacccaa acggcggcga acgccgcgcc gatcagcgac
     1381 atcgcggcga acgcgaatcc gacgctgtcg ctcaagaacg cgaagatgac accccaccac
     1441 gcgtcgcccc cgcgatacac gacgacgtcg acgacgggct tggtcttgaa acgcgtctcg
     1501 cggtcgaccc tcgtgaacag catctcccgc gccggccgcg tgatcccgaa ctcgccggcc
     1561 ctgcgggcga tgtcgaccgc gagcagcacc gcgagcatcg gcgccatcgc gagaatcagg
     1621 aacgtaaccg cgcaaaagac cggaagcaaa gccagcgtga cgggcatgcc gaggcgcttc
     1681 acgatccgat tcgtgacgaa caggccgagg ccgaaggtca ggaggttcac caccgcgtcg
     1741 cggatcgcga ggatcgtcgt tcgctcctcg cgttccgcgt agatgcgcag caagtcggtc
     1801 tgttgaaagt agatgaacgc ctgaatggcc gtgtagatca agatgaacaa accgatcgcg
     1861 agcaggtagg ggctcttcgc gaagtcgtgg aacccctgcc accacttccc gcccatctgc
     1921 gccgcgccga gattcgcttg gagactctcg ttgtgcaaat cgacgacctt gaggcgctga
     1981 atccacagga tcatcgggat cgcgagtagc agggtcgcgg ccccgacgag catcaagttg
     2041 tc
//
LOCUS       DASSNN010000350         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_297929, whole genome shotgun sequence.
ACCESSION   DASSNN010000350 DASSNN010000000
VERSION     DASSNN010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297929"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..150)
                     /locus_tag="VFL84_06435"
     tRNA            complement(74..150)
                     /locus_tag="VFL84_06435"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Arg,seq:acg)
     gene            complement(241..1335)
                     /gene="alr"
                     /locus_tag="VFL84_06440"
     CDS             complement(241..1335)
                     /gene="alr"
                     /locus_tag="VFL84_06440"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7608295.1"
                     /translation="MSTEQGHATSRPATVRIDLDALARNYRMLRERAKPGECAAVVKA
                     DAYGLGVERVAGRLLREGCRRFFVATLSEAQQLRALAPDAEIGVFEGALASTAGALAA
                     IEARPVLSTLEQIERWAGKGRALLHLDTGMNRLGLRAADVATLAQRRDLLDALTLDFV
                     MTHLACADEPGHPLNAEQLARFAALRAVVPTVRTSIGNSAGTLIDAAHRGDLARPGIA
                     LYGGNPFSDRPNPMEAVVTLTAPILQLHDVGEPEPVGYGATYVAAPPARVAIVGIGYA
                     DGYPRSLGNVGAAAVQGTRVPVVGRVSMDLISIDVTAVPRDKVEVGAAVELIGPTVGV
                     DEVAAAAGTISYEILTGLGARLAREYVESD"
     gene            complement(1325..>2234)
                     /locus_tag="VFL84_06445"
     CDS             complement(1325..>2234)
                     /locus_tag="VFL84_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608296.1"
                     /translation="LLSVQAHPPGNTEVYVIVDADLGATIRLGFAVDVDAATLTAKLA
                     GGRRDQQRVLELLGPDSADELQALLKGWLARRAAKPTELKSGLVERLSDATSWAEVDA
                     RLKSLHEMYWTMLDSMNAIAVKAGDVVYNANPPRVVAATGKPASAEVHALGNPEGRGV
                     LALEIRRPGPTLRAWDNVRFPLRKLDIEDALDAVNLTATRPEDFIVERRRVRPGVTRS
                     VDCEYFRLEHLEPTASVAVDVPASPPHCLHALGGCVSVWRGQVEIAALERGESAFAPA
                     RLGAYRIVAEREPATVVRVSLPPYVD"
BASE COUNT          332 a          774 c          786 g          342 t
ORIGIN      
        1 tgaattcgaa cctcctagtt tttcttgtat tcccgcttaa ttacgagccg cgcggacgac
       61 aacaaaagca ggttggcgcg cccggagaga ttcgaactcc cgacctccgg cttcgtaggc
      121 cgatgctcta tccagctgag ctacgggcgc gtgatgactg gctgatggcg aaggcttagg
      181 ggttagtcgt agcgctccgc gggcgccatt ctacccgaag cgggagcccc cgcttcaagc
      241 tcaatcgctt tcgacgtact cgcgggcgag cctggctccc aagcccgtca ggatctcgta
      301 gctgatcgtg ccggccgcgg ccgcgacctc gtcgacgccg accgtgggcc cgatcagctc
      361 gaccgcggcg ccgacctcga ctttgtcgcg cggcactgcg gtcacgtcga tcgagatcag
      421 gtccatcgag acgcgtccaa cgacgggcac gcgcgtgccc tgcacggccg ccgcgcccac
      481 gttgccgagg ctgcgcggat agccgtccgc atagccgatg ccgacgatcg cgacgcgcgc
      541 cggaggcgcg gcgacgtacg tggcgccgta gccgacgggc tcgggctcgc cgacgtcgtg
      601 gagctgcagg atcggcgccg tcaacgtgac gacggcctcc atggggttcg gccgatcgct
      661 gaacggattg ccgccgtaga gcgcgatgcc ggggcgcgcg agatcgccgc ggtgcgccgc
      721 gtcgatcagc gtgccggccg agttgccgat cgaggtccgc acggtcggca cgaccgcgcg
      781 cagcgcagca aagcgcgcga gctgctcggc gttcagcgga tggccgggct cgtcggcgca
      841 cgcgagatgc gtcatgacga agtcgagcgt gagcgcgtcg agcaggtcgc ggcgctgcgc
      901 gagcgttgcg acgtcggcgg cgcgcaagcc gaggcggttc atgcccgtgt cgaggtgcag
      961 cagcgcgcgg ccttttccgg cccaacgctc gatttgctcg agcgtgctca gcacgggccg
     1021 tgcttcgatc gcggcgagcg cgccggccgt gctcgctagc gcgccctcga acacgccgat
     1081 ctcggcgtcc ggcgcgagag cgcgcagctg ctgcgcttcg gacagcgtcg cgacgaagaa
     1141 ccgtctgcat ccttcgcgca gcaagcgtcc cgcgacgcgc tccactccga gcccgtaagc
     1201 gtcggctttg acgacggcgg cgcactcgcc cggcttcgcg cgctcgcgca gcatcctgta
     1261 gttgcgcgcc aacgcgtcga gatcgatgcg caccgtcgca ggccgcgacg ttgcgtggcc
     1321 ctgctcagtc gacatacggc ggcaggctca ctctgacgac ggtcgcgggc tcgcgttcgg
     1381 cgacgatgcg atacgcgccg agcctggccg gagcgaacgc cgactcgccg cgctcgagtg
     1441 ctgcgatctc gacttggccg cgccacacgc tgacgcagcc cccgagcgca tgcaggcaat
     1501 gcggcggcga agccggcacg tcgacggcga cgctcgccgt cggctcgagg tgctcgagcc
     1561 gaaagtactc gcaatcgacg gatcgcgtga cgccgggccg cacgcgcctc cgctcgacga
     1621 tgaagtcctc gggccgcgtc gcggtgagat tcacggcgtc gagcgcgtct tcgatgtcga
     1681 gcttgcgcag cggaaagcgc acgttgtccc acgcgcgcag cgtcgggccg gggcgccgga
     1741 tctcgagcgc gagcacgccg cggccttcgg ggttgccgag cgcgtgcact tccgccgagg
     1801 cgggcttgcc ggtcgcggcg acgacgcgcg gcgggttcgc gttgtaaacg acgtcgcccg
     1861 ctttgacggc aatcgcgttc atcgaatcga gcatggtcca gtacatctcg tgcagcgact
     1921 taagccgcgc gtcgacttcg gcccagctcg tcgcgtcgga gagccgttcg acgagccccg
     1981 acttgagctc ggtcggcttc gccgcgcggc gagcgagcca acctttcagc aacgcctgaa
     2041 gctcgtcggc cgaatccggg ccgagcagct cgagcacgcg ctgctgatcg cgccggccgc
     2101 cggcgagctt ggcggtcagc gtcgcggcgt cgacgtcgac ggcgaagcct aagcggatcg
     2161 tcgcgcccaa gtcggcatcg acgatgacgt agacctcggt gttgcccggt ggatgagcct
     2221 gcacggacag gagc
//
LOCUS       DASSNN010000351         3398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_193189, whole genome shotgun sequence.
ACCESSION   DASSNN010000351 DASSNN010000000
VERSION     DASSNN010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193189"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1301
                     /gene="odhB"
                     /locus_tag="VFL84_06450"
     CDS             3..1301
                     /gene="odhB"
                     /locus_tag="VFL84_06450"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HET7608297.1"
                     /translation="MPESVTDATLVAWHKKAGETVRRDENLVDLETDKVVLEVPAPVD
                     GVIREIVANDGAVVTSGQVLAILEPGAAGAAAAPPAKDKDKAPAAAKPAPAPKPESAP
                     RPAAAAPAAPAAVPAQARSTAAANKMGPAVRKLVEEHDLDPAAISGSGREGRITKGDV
                     LDYIADHQATHVSIEHDVVSRAGAAKQPAPPIPQPAGQGSRNEHRVAMTRLRARIAER
                     LLEAQQQAAMLTTFNEVDMTAVMALRQRYQEQFTAKHGIKLGFMSFFVKASVEALRQF
                     PVINASVDGSDIVYHEFFDIGVAVSTDRGLVVPVLRNAEAMSFGAIEQKIRDFGERAR
                     KGALQMEELTGGTFTITNGGIFGSLMSTPILNPPQSGILGMHKIQERAVVVGGKIEAR
                     PMMYLALTYDHRIVDGREAVQFLVHVKDLLEDPARLLLEI"
     gene            1320..2747
                     /gene="lpdA"
                     /locus_tag="VFL84_06455"
     CDS             1320..2747
                     /gene="lpdA"
                     /locus_tag="VFL84_06455"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007984586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET7608298.1"
                     /translation="MSDKNFDVVVIGGGPAGYPAALRAAQLGLSAACIDDYKNEEGTR
                     LFGGTCLNVGCIPSKALLESSELYHRAHTEFELHGIRVAGVGIDVEAMQARKRGIVKQ
                     LTSGIEAMFKAGGVTGFKGRGKLLAGNRVEVTTEAGVETLAAQHVVLATGSAPIELSI
                     AKFDGQRIVDSTGALAFNNVPKRLGVIGAGVIGLELGSVWKRLGSEVVILEALETFLP
                     MVDQQVAKDALRHLKKQGLDIRLGAKVTAAAVEGNAVKVRYEMGGAAQDLTVDALVVA
                     VGRKPVTAGLFAPDVKVPLDKRGFIEVDEHCRTTVPNVWAVGDVVRGPMLAHKGMEEG
                     VMVAELIAGHVAEMNYRTIPSVIYTAPEIAWVGETEEEVKASGRQYKVGTFSFAANGR
                     AKGMQQTAGFVKLIADKEHDDVLGVHIVGPVAGELIAEAVLAMEFSASAEDIQRTIHA
                     HPTLSEALHEASLAADKRAIHGINR"
     gene            2911..>3398
                     /gene="ruvC"
                     /locus_tag="VFL84_06460"
     CDS             2911..>3398
                     /gene="ruvC"
                     /locus_tag="VFL84_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET7608299.1"
                     /translation="MSTRVASPIGAAPLASVIGSVRILGIDPGSQVTGFGVVDVAGNR
                     TTAVEWGSVRLEGGHSDRLRSIFTALKQIVHEYRPAEIAIERVFLSRNADSALKLGQA
                     RAAAICATFESNVPIYEYSARHIKKAVVGRGAAEKDQVQRMVQMILGVREAIAADAAD
                     ALA"
BASE COUNT          592 a         1143 c         1171 g          492 t
ORIGIN      
        1 aattgcccga gtccgttacc gacgcgacgc tcgtcgcctg gcacaagaag gccggcgaaa
       61 ccgtgcgacg cgacgagaac ctcgtcgatc tcgagaccga caaggtcgtg ctcgaggtgc
      121 cggcccccgt cgacggcgtg atccgcgaga tcgtcgcgaa cgacggcgcc gtcgtgacga
      181 gcgggcaggt gctcgcgatt ctcgagccgg gcgccgccgg ggcggccgcc gcgccgccgg
      241 ccaaggacaa ggacaaggcg cccgccgcgg cgaagccggc gcccgcgccc aaaccggaga
      301 gcgcgccgcg ccccgccgcc gcagccccgg ccgctcctgc cgcggttccg gcgcaggcac
      361 gctcgaccgc cgccgcgaac aagatgggcc ccgccgtgcg caagctcgtc gaggagcacg
      421 acctcgatcc cgccgcgatc tcgggctcgg ggcgcgaagg ccgcatcacg aagggcgacg
      481 tgctcgacta catcgccgac caccaggcca cgcacgtcag catcgagcac gacgtcgtga
      541 gccgtgccgg cgcggcgaag cagccggcgc cgccgatccc gcagccggcc ggtcaaggct
      601 cgcgcaacga gcatcgagtc gcgatgacgc ggctgcgcgc gcgcatcgcc gagcgcttgc
      661 tggaagcgca gcaacaagcc gcgatgctga cgaccttcaa cgaggtcgac atgacggccg
      721 tcatggcgct gcggcagcgc taccaagagc aattcacggc gaagcacggc atcaagctcg
      781 gcttcatgtc gttcttcgtc aaagcttcgg tcgaagcgtt gcgccagttc cccgtcatca
      841 acgcgtcggt cgacggcagc gacatcgtct accacgagtt cttcgacatc ggcgtcgccg
      901 tctcgacgga ccgcgggctc gtcgtgcccg tgctgcgcaa tgccgaggcc atgagcttcg
      961 gcgccatcga gcaaaagatc cgcgacttcg gcgagcgcgc gcgcaagggc gcgctgcaaa
     1021 tggaagagct gacgggcggc acgttcacga tcacgaacgg cggcatcttc ggctcgttga
     1081 tgtcgacgcc gatcttgaat ccgccgcaaa gcggcattct cggcatgcac aagatccaag
     1141 agcgcgccgt ggtcgtcggg ggcaagatcg aagcccgccc gatgatgtat ttggcgctga
     1201 cgtacgatca ccgcatcgtc gacggccgcg aagccgtaca attcctcgtg cacgtgaagg
     1261 atctgctcga ggatccggcg cggctcctgc tcgaaatcta agcaaccgta aagtagacaa
     1321 tgagcgacaa aaacttcgac gtcgtcgtca tcggcggcgg tccggccggc tatcccgcgg
     1381 cgctgcgcgc ggcacagctc ggactctcgg cggcctgcat cgacgactac aagaacgagg
     1441 agggcacccg gctcttcggc ggcacctgct tgaacgtcgg ctgcatcccg tcgaaggcgt
     1501 tgctcgagtc ctccgagctc tatcaccgcg cgcacaccga gttcgagctg cacggcattc
     1561 gcgtggcggg cgtcggcatc gacgtcgagg cgatgcaggc gcgcaagcgc ggcatcgtca
     1621 agcagctcac gtccggcatc gaggcgatgt tcaaggccgg cggcgtgacg gggttcaagg
     1681 gccgcggcaa gctgctcgcc ggcaatcgcg tcgaggtcac gaccgaggcc ggcgtcgaga
     1741 cgctcgcggc gcagcacgtc gtgctcgcga cgggctccgc gccgatcgag ctctccatag
     1801 cgaagttcga cggccaacgc atcgtcgatt cgacgggcgc gctcgcattc aacaacgtgc
     1861 cgaagcggct cggcgtcatc ggcgccggcg tcatcggcct cgagctcggc tcggtctgga
     1921 agcgcttggg ctccgaggtc gtgatcctcg aggcgctcga gacgttcctg ccgatggtcg
     1981 accagcaggt cgcgaaggac gcgctgcggc atctgaagaa gcaggggctc gacatccgcc
     2041 tcggcgcgaa ggtcacggct gccgccgtcg agggcaacgc cgtcaaggtg cgttacgaga
     2101 tgggcggcgc cgcgcaggat ctcaccgtcg acgcgctcgt cgtcgccgtc ggccgcaagc
     2161 cggtgacggc ggggctcttc gcgcccgacg tcaaggtgcc gctcgacaag cgcggcttca
     2221 tcgaggtcga cgagcactgc cgcacgaccg tgccgaacgt ctgggccgtc ggcgacgtcg
     2281 tccgcgggcc gatgctcgcg cacaagggaa tggaagaagg cgtcatggtc gccgagctga
     2341 tcgcggggca cgtcgccgag atgaactacc ggacgatccc gtccgtgatc tacacggcgc
     2401 ccgagatcgc gtgggtcggc gagaccgaag aggaagtcaa agcgtcgggc cggcagtaca
     2461 aggtcggaac cttcagcttc gccgcgaacg gccgcgccaa gggcatgcag cagacggccg
     2521 gcttcgtcaa gctcatcgcc gacaaggagc acgacgacgt gctcggcgtg cacatcgtcg
     2581 ggcccgtcgc cggcgagctc atcgccgagg ccgtcctcgc catggagttc tcggcctcgg
     2641 ccgaggacat ccagcgcacg atccacgccc acccgacgct cagcgaggcc ttgcacgagg
     2701 cgtcgctcgc cgccgacaag cgcgccattc acggcatcaa ccgctaacgc tcgttcgagc
     2761 gttgccgtta tcattggccg cgggtggctt gggggccgcc tcggagtagg ggagtttacg
     2821 gaagcgtgtc gccggccttc gcgtggcggg cggcatgcat tggtgcgaca tgccgcagag
     2881 tcgatcggcc gcgttcgcag aggtgtgcgt gtgagcacgc gggtggcgag cccaatagga
     2941 gcagcgcctc tcgccagtgt catcggttcc gtccgcattc tcgggatcga tccgggctct
     3001 caggtcacgg gcttcggcgt cgtcgacgtc gccggcaacc gcacgacggc cgtcgagtgg
     3061 ggcagcgttc gcttggaagg cgggcacagc gatcgcttgc gcagcatctt cacggcgctg
     3121 aagcagatcg ttcacgagta ccgcccggcc gagatcgcga tcgagcgcgt gttcttgagc
     3181 cgcaacgccg acagcgcgtt gaagctgggc caagctcgcg cggcggcgat ctgcgcgacg
     3241 ttcgagtcga acgtgccgat ctacgagtac agcgcgcgac acatcaagaa agcggtcgtc
     3301 ggccgcggag cggccgagaa ggatcaggtg caacgcatgg tgcagatgat tctgggagtt
     3361 cgagaagcga tcgcggccga tgccgcggac gcgctcgc
//
LOCUS       DASSNN010000352         3474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1194848, whole genome shotgun sequence.
ACCESSION   DASSNN010000352 DASSNN010000000
VERSION     DASSNN010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194848"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2274)
                     /locus_tag="VFL84_06465"
     CDS             complement(<1..2274)
                     /locus_tag="VFL84_06465"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET7608300.1"
                     /translation="MSGDRRRIPEFYKLSVEERVRAVHERGLINNADFRALATGQHTL
                     DLDSADKMIENVVGVMGMPLGLGMNLVVNEQQYIVPMVVEEPSVVAALGSACKLIRNC
                     GGFKAEATDPILIGQIQIVNLADMEKAKQALRDHKQDIINLANSLHPNMVARGGGARD
                     LELFVHPLPSSAKQMLVVHLLVDTRDAMGANLVNGMCEGVAPLVESITGGEVFLRILS
                     NLADRSLVRAHCKIPVEQLAGKGYTGVQARDGIIVAGEFAAVDPYRAATHNKGIMNGI
                     DPIAIATGNDWRAIEAGAHAYAARGGRYSSLTDWWADEEGNLCGRIELPIKVGIVGAP
                     IESNPTVALNLRLLGVSSAVELAQVMAAAGLAQNFAAIRALATEGIQKGHMTLHARSV
                     VTAAGAPKEIFDEVLERLIQSGVIKVWKAQELVAEMQDERARAAAGPKRKRAKEAAMG
                     VGYGKVILLGEHAVVYGRHAIAAPIPLKIKALVEDCDEGIHLLIPRWNVEYRLASNPN
                     ERRSFERPAGALLDHLGLSKRAMKIEVFPEVPRSMGLGGSAAMAVAIVRALDKHFRLR
                     LSDAEVNRLAFESEKLAHGSPSGLDNTLACYGKALVFRAGDPPLVEPLNIREPIPAVI
                     GMTGLEGLTAKTVGHVREAWQRDNKLYERIFDQIDALTLRGIQAIQDNDLLTLGQLMN
                     ICQGMLNALQVSTPELEKICAIARENGALGAKLTGGGGGGSIVAICDGDPEPVVTAIR
                     AAGFQAMPVHLGAELNGA"
     gene            complement(2271..3275)
                     /locus_tag="VFL84_06470"
     CDS             complement(2271..3275)
                     /locus_tag="VFL84_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608301.1"
                     /translation="MIVARAPGKLVALGEYAVLDGAPAVVLALDRYAEIEVAESADGA
                     CRLTTRSYEVVERTFRPGEPSGAGLVDLVTAAAPRLAWTATIDSQAFFAGRLKLGLGS
                     SAAVLAAWAGAFAAFARGQGEPVPEPRVEALIELHRRFQGGQGSGLDVAASYTGGAIM
                     FRLAGPDLPQIGSVRLPNSVGFAGIFAGRSASTAELVSRYRAWRKDRPQEATQLLRRL
                     TAQAERGCAALTGDDAAGWLEAFEAYGQGLGELGRAIGADIVTAEHREIGVEARRHGV
                     AYKVSGAGGGDLGLACAADRDALEAFSQSVGDRGFRVIKVSLADHGLQVAQRGAGWQR
                     "
     gene            complement(3276..>3474)
                     /locus_tag="VFL84_06475"
     CDS             complement(3276..>3474)
                     /locus_tag="VFL84_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET7608302.1"
                     /translation="TVECLHAVRRLRSAGVPVFFTIDAGPQLKAVCAPGARAEVERTL
                     RAVPGVLELLTSALGPGAELK"
BASE COUNT          532 a         1226 c         1142 g          574 t
ORIGIN      
        1 cgcgccattc aattcggctc cgagatggac gggcatcgcc tggaaaccgg cggcccgaat
       61 cgcggtgacg accggctccg ggtcgccgtc gcagatggcc acgatcgagc cgccgccgcc
      121 gccgcccgtc agtttcgcgc cgagcgcgcc gttctcgcgc gcgatcgcgc agattttctc
      181 gagctcgggc gtcgatacct gcagggcgtt cagcatgccc tgacatatat tcatgagctg
      241 gcccaaggta agcaagtcgt tgtcttgtat ggcctgaatg ccgcgcagcg tcagcgcgtc
      301 gatctggtcg aaaatccgct catataactt attgtcgcgt tgccaggcct cgcggacgtg
      361 gccgacggtc ttggccgtca ggccctcgag gcccgtcatc ccgatgacgg ccgggatcgg
      421 ctcgcggatg ttcaacggct cgacgagcgg cgggtcgccg gcgcggaaca cgagcgcctt
      481 gccgtagcac gcgagcgtgt tgtcgaggcc gctcgggctg ccgtgcgcga gcttctcgga
      541 ctcgaacgcg aggcgattga cctcggcgtc gctcaagcgc aggcggaaat gcttgtcgag
      601 cgcgcgcacg atcgcgacgg ccatcgcggc cgagccgccg aggcccatgc tgcgcggaac
      661 ttcagggaag acctcgatct tcatcgcgcg cttgctcaag ccgagatggt cgagcaaggc
      721 gccggccggg cgctcgaacg agcgccgctc gttcggattg ctcgcgagcc ggtattcgac
      781 gttccagcgc gggatcaata gatggatgcc ttcgtcgcaa tcctcgacga gcgctttgat
      841 cttgagcggg atcggcgcgg caatcgcgtg ccggccgtac acgacggcgt gctcgccgag
      901 caagatgacc ttgccgtagc cgacgcccat cgcggcctct ttggcgcgct tgcgtttcgg
      961 acctgccgcc gcgcgcgcgc gctcgtcctg catctccgcg acgagctcct gcgctttcca
     1021 gactttgatg acgccgctct ggatcagccg ctcgagcacc tcgtcgaaga tttccttcgg
     1081 cgcgcccgcg gccgtgacga cgctgcgcgc gtgcagcgtc atgtgtcctt tctggatgcc
     1141 ttccgtcgcg agcgcgcgaa tcgcggcgaa gttctgcgct agacccgccg ccgccatgac
     1201 ttgcgcgagc tcgacggccg agctcacgcc gagcaagcgc aggttcagcg cgacggtcgg
     1261 attcgattcg atcggcgcgc cgacgatgcc gactttgatc ggcagctcga tgcggccgca
     1321 gaggttgccc tcctcgtcgg cccaccaatc cgtcaacgac gagtagcgcc cgcctcgagc
     1381 cgcgtacgcg tgcgcgccgg cctcgatggc gcgccagtcg ttgcccgtcg cgatcgcgat
     1441 cgggtcgatg ccgttcatga tgcctttgtt gtgcgtcgcc gcgcggtacg gatcgacggc
     1501 ggcgaactca cccgcgacga tgatgccgtc gcgtgcctgc acgccggtat agcccttgcc
     1561 ggcgagctgc tcgacgggaa tcttgcaatg cgcccgcacg agcgagcgat ccgcgagatt
     1621 cgacaggatg cgcaagaaca cctcgccgcc cgtgatcgac tcgacgagtg gcgccacgcc
     1681 ctcgcacatg ccgttgacga gattcgcgcc catcgcgtcg cgcgtgtcga caagcaggtg
     1741 cacgacgagc atctgcttcg cgctcgacgg cagcgggtgc acgaacagct cgagatcgcg
     1801 cgcaccgccg ccgcgggcga ccatgttcgg atgcaggctg ttggcgagat tgatgatgtc
     1861 ttgcttgtgg tcgcgcagcg cctgcttcgc tttctccatg tcggccaagt tcacgatctg
     1921 gatctggccg atcaggatcg gatccgtcgc ttcggctttg aagccgccgc agttgcggat
     1981 cagcttgcag gcggaaccga gcgccgcgac gacggagggt tcctcgacga ccatcggcac
     2041 gatgtattgc tgctcgttga cgaccaagtt catgccgagc ccgagcggca tgcccatgac
     2101 gccgacgacg ttctcgatca tcttgtcggc ggaatcgagg tcgagcgtgt gctgccccgt
     2161 cgcgagcgcg cggaagtcgg cgttattgat gaggccgcgc tcgtggacgg cgcggacgcg
     2221 ctcctcgacg ctcaacttgt agaactcggg aatacgcctt cggtcaccgc tcatcgctgc
     2281 caccccgcgc cgcgttgtgc tacctgaagg ccgtggtcgg ccagcgagac tttgatcacg
     2341 cgaaaacccc gatcccctac cgattgactg aacgcctcga gcgcatcgcg gtcggccgcg
     2401 cacgcgagcc caaggtcgcc gccgcccgcg ccgctgacct tgtacgcgac tccatggcgc
     2461 cgcgcctcga cgccgatctc acggtgctct gccgtgacga tatcggcgcc gatcgcccgg
     2521 ccgagctcac ccaagccctg gccgtaggct tcgaacgctt cgagccatcc cgcggcgtcg
     2581 tcccctgtga gcgccgcgca tcctctttct gcctgcgccg tcaaacggcg cagcagctgc
     2641 gtcgcttcct gcggccgatc ctttcgccaa gcccgataac gcgatacgag ctcggctgtc
     2701 gaggcggacc ggccggcaaa aatacccgcg aatcctacac tgttcggcag gcggactgaa
     2761 ccaatttggg gcagatcggg acccgcgagc cggaacatga tcgccccgcc cgtgtacgag
     2821 gcggcgacgt cgagaccgct gccctggccg ccttggaacc gccggtgtag ctcgatcagc
     2881 gcttcgaccc gcggctcggg caccggctcg ccctggcccc gggcgaacgc cgcgaacgcg
     2941 ccggcccagg cagcgagcac cgcggcgctc gagcccagac cgagcttcaa gcggccggca
     3001 aagaacgctt gcgagtcgat cgtcgccgtc cacgcgagtc gcggcgccgc cgccgtgacg
     3061 agatcgacga gcccggcgcc gctcggctcg cccggccgga acgtgcgctc gacgacctcg
     3121 tacgagcgcg tcgtcagccg gcaagcgccg tccgcgctct cggcgacctc gatctcggcg
     3181 tagcggtcga gcgcgaggac gacggcgggc gcgccatcga gcaccgcgta ctcgccgagc
     3241 gccacgagct tgcccggcgc gcgcgcgacg atcacttact tgagctccgc gccgggtccg
     3301 agggcgctgg tcaggagctc gagcacaccc ggcaccgcgc gcagcgtgcg ctcgacttcc
     3361 gctcgagcgc cgggcgcgca gaccgccttg agctgtgggc ccgcgtcgat cgtgaaaaac
     3421 acgggcacgc cggccgagcg caggcgccgc accgcgtgca ggcattcgac cgtc
//
LOCUS       DASSNN010000353         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1576441, whole genome shotgun sequence.
ACCESSION   DASSNN010000353 DASSNN010000000
VERSION     DASSNN010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576441"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFL84_06480"
     CDS             complement(<1..827)
                     /locus_tag="VFL84_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608303.1"
                     /translation="MLAETLVRWRIAREQRRNAKLTRAELDAAKLEKFRRLVRYIEPR
                     SAYYRRIVAERRINLDTCTPSDFPVLTKSLLMKHFDEIATAPGVTKSAIAEFLTRSHE
                     PMELFLGRYRVIHTSGSSGEVGYFVYSSSDWARGTTMRRQGGRPVRKGGAKGKFKVAY
                     YAAVDGHYAGVTLISSFKTGLARFFADLEMFEVNDPLPDVVARLNAFQPEVLIGYTTA
                     LKILAAEQRRGTLKLERVINVTTAGEATTSADMATLSETFGCQVVNSYGCSEHLGVGA
                     "
     gene            complement(845..1819)
                     /gene="pyrB"
                     /locus_tag="VFL84_06485"
     CDS             complement(845..1819)
                     /gene="pyrB"
                     /locus_tag="VFL84_06485"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HET7608304.1"
                     /translation="MFHVIDPRLFERPLLDELCALATRARLAAKSEAGSRALRDLLPT
                     RRAMLYFTQPSTRTFLSFNNACHILGMRTSEIRDPSTSSEVKGESFEDSIRTFSSYVD
                     VIIMRTKEAGRAAQAAQLMNGIPRPVPVINAGSGSDQHPTQALLDIYTLQRSFEGHGG
                     IDGKVIGMMGDLKRGRTVRSLCYLMKNYYGVRLVFIAPEQFAMEPDVKAHLEEHRISY
                     HETTRLDEALPSLDALYVTRMQTEWDTAGESRNVDLTRFSIGAPQLARLKRDAIIMHP
                     LPRGPEIDPAVDQDPRAMYWRQERNGMWMRAAVLAKIFGAEDRIPAPR"
     gene            1996..>2559
                     /locus_tag="VFL84_06490"
     CDS             1996..>2559
                     /locus_tag="VFL84_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7608305.1"
                     /translation="MRTRLVVTWLCALGSLAAVADLAAQSTASTGTAAQADPTNLVLV
                     GNRFAPLKWTEMSEQQKTMMRHVLDGPRTAANGPFNVMLRAPLMGDYAQELGAQVRFN
                     SSLPAPLREMAILMAARHWTAHYEWNAHKAGALAAGLNPDIVAAIAAGERPKSMQPNE
                     AALHRFCSELLETTRVGDDTFAAAKAAF"
BASE COUNT          408 a          828 c          879 g          444 t
ORIGIN      
        1 gcgcccacac ctaagtgctc gctgcagccg tagctgttca cgacctggca accaaaggtc
       61 tcgcttagag tcgccatgtc ggccgatgtc gtcgcttcgc cggccgtcgt gacattgatg
      121 acgcgttcga gcttcagcgt cccgcgccgc tgctcggccg cgagaatctt gagagcggtc
      181 gtgtaaccga tcagcacctc gggttggaac gcgttgagcc gcgcgacgac gtccggaagc
      241 gggtcgttga cctcgaacat ctcgaggtcc gcaaagaatc tcgcgagccc ggtcttgaac
      301 gagctgatca gcgtgacacc ggcgtagtgg ccgtcgacgg cggcgtaata cgcgaccttg
      361 aacttgccct tcgcgccgcc cttgcgcacg gggcgcccgc cctgccggcg catcgtcgtg
      421 ccgcgcgccc aatcggagct cgagtacacg aagtagccga cctcacccga cgatcccgat
      481 gtgtggatca cgcggtagcg gccgaggaag agctccatgg gctcgtgcga tcgcgtcagg
      541 aactccgcga tcgcgctctt cgtgacgccg ggggccgtcg cgatctcgtc gaagtgcttc
      601 atcagcagcg acttcgtgag cacggggaaa tcgctgggcg tgcaggtgtc gaggttgatc
      661 cggcgttcgg cgacgatgcg gcggtagtag gcggaacgcg gctcgatgta gcgcacgagg
      721 cgccggaatt tctcgagctt cgcggcgtcg agctccgcgc gcgtgagctt ggcgttgcgc
      781 cgctgttctc gggcaatgcg ccaccggacc aaggtctcgg cgagcatgtc ggcgggcccc
      841 gcggctaccg aggcgcgggg atacggtctt cggcgccgaa gatcttcgcg agcacggccg
      901 cgcgcatcca catgccgttg cgctcctgcc gccagtacat cgcgcgcggg tcctggtcga
      961 cggcggggtc gatctcgggg ccgcgcggca gcgggtgcat gatgattgcg tcgcgcttca
     1021 agcgcgcgag ctgcggcgcg ccgatgctga agcgcgtgag gtcgacgttg cgcgattcgc
     1081 cggccgtgtc ccattccgtt tgcatgcgcg tcacgtacag cgcgtcgagc gacggcagcg
     1141 cctcgtcgag ccgcgtcgtc tcgtggtacg aaatgcggtg ctcttcgaga tgcgctttga
     1201 cgtcgggctc catagcaaac tgctcgggcg caatgaacac gagccgcacg ccgtagtagt
     1261 ttttcatcag gtagcagagc gagcgcacgg tgcggccgcg cttcaagtcg cccatcatgc
     1321 cgatgacttt gccgtcgatg ccgccgtgcc cctcgaagct gcgttgcagc gtgtagatgt
     1381 cgagcaaggc ttgcgtcggg tgctgatcgc tgccgctgcc ggcgttgatg accgggacgg
     1441 gccgcggaat gccgttcatg agctgcgccg cctgcgccgc gcgcccggct tccttcgtgc
     1501 gcatgatgat gacgtcgacg tacgagctga acgtgcggat cgaatcttcg aagctctcgc
     1561 ccttgacctc cgacgacgtc gagggatcgc ggatctcgct cgtgcgcata cccaagatgt
     1621 ggcacgcgtt gttgaacgag aggaacgtcc gcgtcgacgg ctgagtgaag tagagcatgg
     1681 ccctgcgcgt cggcaacaga tcgcgcagcg cacgcgaccc agcttccgat ttcgcggcga
     1741 gccgcgcgcg cgttgccagc gcgcagagct cgtcgagcag cggccgctcg aacaggcgcg
     1801 ggtcgatgac gtggaacaac ggcgaggttc gcgtcggcag gaccagcgtt tcgctcaggg
     1861 gttcggtggc gatagcggta tcctctgaag ggtggtacgg cgcgtggcgt gcaccgaaat
     1921 tgtacgctgc aacgcgcgcg tggtgtatcg tccgctcgca ggccctctga accacaactg
     1981 ctccggagcc agcccatgcg tactcgactc gtcgtgacgt ggctttgcgc cttgggttcc
     2041 ttagccgcgg tagccgatct cgcggcgcag agcacggctt ccaccgggac ggccgcgcag
     2101 gccgacccca cgaacctagt gctcgtcggc aatcggtttg cgccgctgaa gtggaccgag
     2161 atgtctgaac agcagaagac gatgatgcgc cacgtgctcg acggcccgag gacggcggcg
     2221 aacggcccgt tcaacgtcat gttgcgcgcg ccactcatgg gcgactacgc ccaagagctc
     2281 ggcgcgcaag tccgcttcaa ctcgtcgctg cccgcgccgc tgcgcgagat ggcgatcctg
     2341 atggccgcgc gtcattggac ggcgcactac gaatggaacg cgcacaaggc gggcgcgctc
     2401 gcggcaggcc tcaatcccga catcgtcgcg gcgatcgcgg ccggcgagcg gccgaagtcg
     2461 atgcagccga acgaggcggc gctgcatcgc ttctgctctg agctgctcga gacgacgcgc
     2521 gtcggcgacg acacgttcgc ggcggccaag gccgcattc
//
LOCUS       DASSNN010000354         4552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_65126, whole genome shotgun sequence.
ACCESSION   DASSNN010000354 DASSNN010000000
VERSION     DASSNN010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65126"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFL84_06495"
     CDS             <1..534
                     /locus_tag="VFL84_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HET7608306.1"
                     /translation="APALALGVDPPPPGVMARPPRPRGEGVITGRMWAGIFYVGTIMA
                     AGTLLVLDASLPGGLIEGSGDERYAQTMALTTLVLLHLLTVFNARSDERSAFAELFSN
                     KWLWCALLLSLALQAAVLYVPFLQHAFSTVALSARDWLACAAVASSVLWLRELSKLFT
                     AGRFRASTPGSGARSGR"
     gene            634..987
                     /locus_tag="VFL84_06500"
     CDS             634..987
                     /locus_tag="VFL84_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5335 domain-containing protein"
                     /protein_id="HET7608307.1"
                     /translation="MAISKLEKDAWRPYFDRVSKLLGGTKRAEIEVAALNLGDQIEAE
                     WAPLTGIVYDPKDDLIEVVLEDLDHMIHRPQQVFIDQDAVLLKSVEVIDESGVRQIIK
                     LRDPLTLPPPERAQH"
     gene            complement(1038..2297)
                     /gene="glp"
                     /locus_tag="VFL84_06505"
     CDS             complement(1038..2297)
                     /gene="glp"
                     /locus_tag="VFL84_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HET7608308.1"
                     /translation="MAQLSQDCFASGAEILSVDDAVAAITARLAHVEGVETVALAEAD
                     GRVLGDDLVATLQLPPFTNSAVDGYAVRGEDLPSGAARLFRVSGRVQAGSASAAATVA
                     AGEAVRIFTGAPMPKGTDTVFMQEDARLDDAGRVLLPAGLERGANVRPAGEDIALGRV
                     VLPAGRWLRPQDVAAAAAMGLTSIRVRRRVKVAVFSNGDEVVEPGSARRDAQLYDSNR
                     LMLIAMLRRIGCSVGDLGIRRDDSAQIAEVLKAAARSNDLILSSGGVSTGEGDFVKAA
                     VEKVGTLVFWRIAMKPGRPVAMGVIDGTPFIGLPGNPVASFVTFARVARPAIFTLAGA
                     QWSPPPAIPVRVQFAHRKKPARREYVRVYLRKSEDGTLEAVKFPREGAGLLSSLVDTD
                     GVVELGESVTRVEPGQMVDCFTYASLL"
     gene            complement(2306..3178)
                     /gene="fdhD"
                     /locus_tag="VFL84_06510"
     CDS             complement(2306..3178)
                     /gene="fdhD"
                     /locus_tag="VFL84_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HET7608309.1"
                     /translation="MGDIDAVGRRQSARPLEVRRFRGGELGTSADPDLVATEEPFEIR
                     LGYSRRDGSRAEEPVSITMRTPGHDEDLAVGFLFTEGIIRARTDVQGVVARGQRAADG
                     LINVVRVELAPGVEVDFKRLERNFYMTSSCGVCGKASIDAVAVQGQYDVGATDFRMAG
                     SALGRLPAALKTKQAVFEQTGGLHASGLFDSTGEIVALREDVGRHNALDKLIGHALMH
                     DEVPLKQSGVIVSGRASFELMQKAKMAGVPLVAAVGAPSSLAVELAREFGITLVGFLK
                     ADRFNVYSRPDRIV"
     gene            complement(3166..4275)
                     /locus_tag="VFL84_06515"
     CDS             complement(3166..4275)
                     /locus_tag="VFL84_06515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014954010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET7608310.1"
                     /translation="MPAPMFHYIDGAADDEWTLRRNTAAFDGVEFLPRALVDVSTIDM
                     STTLFGQKIEWPYFCSPTALQRLFHYEGEGGTARAAHASGTIFSLSSVSSTNIEDAAK
                     LSPGPKVFQVYMFRDRGLSREFVQRARDSGYVALQLTVDVTVSGNRERDIVTGMTVPP
                     KLALMSLIDIAMKPKWVYKHLTTPKIEVANIAHRPPPGSDKLGGIIQFLNAQIDRSIT
                     WKDAEWMIKQWGGPFAIKGVMTGEDAKRAHDIGASAVMVSNHGGRQLDFSPAPFDVLK
                     EIVDAAGDRLEVILDGGVRRGTHILKALALGARACSAGRPHLYGLGAGGEAGVHQALK
                     ILKTELARDMALLGCPNVRAIDSSFVRRVGGSWAT"
BASE COUNT          721 a         1559 c         1534 g          738 t
ORIGIN      
        1 gcgccggcgc tcgcgctcgg cgtcgacccg ccaccgcccg gggtgatggc ccggccgccg
       61 cgcccgcgcg gcgaaggcgt gatcaccggc cgcatgtggg cgggcatctt ctacgtcggc
      121 accatcatgg cggcgggaac ccttctcgtg ctcgatgcga gccttccggg cgggctgatc
      181 gaggggtcgg gcgacgagcg ctacgcgcag acgatggctt tgaccacgct ggtgctgctc
      241 cacctgctca ccgttttcaa tgcgcgctcc gacgagcgca gcgccttcgc ggagctgttc
      301 tccaacaagt ggctgtggtg cgcgctgctg ctgtctctcg ccctgcaggc cgcggttctc
      361 tacgtgccgt tcctgcagca tgcgttttcg accgtcgcgc tcagcgcgcg cgattggctg
      421 gcttgcgcag cggtggcgag ctcggtgctg tggctgcgcg agctgagcaa gctatttacg
      481 gccggccgat tccgcgcttc aactcccgga agcggtgcgc gcagcggacg ctaaagagga
      541 gatttcgagc aacagggccc tgcgaatcgc gaacgggacc gtggcccgcc tgtcgaagac
      601 attagctgcc tcttgcagcg acggagaccc tccatggcca tatcgaagct cgagaaggac
      661 gcatggcgtc cttactttga ccgcgtgtcg aaactgctcg gcggcaccaa gcgcgcggaa
      721 atcgaggtcg ccgcgctgaa cctcggcgat cagatcgagg cggaatgggc gccgctgacc
      781 ggcatcgtct acgacccgaa ggacgacctg atcgaggtcg ttctcgagga cctggatcac
      841 atgatccacc ggccgcagca ggtgttcatc gaccaggacg cggtgctcct gaagagcgtg
      901 gaagtgatcg acgagagcgg cgtgcggcag atcatcaaat tgcgtgatcc gctgacgttg
      961 ccgccgcccg agcgtgcgca gcactgaata ggcgtcgctc gcttgcggcg cgggcggtgt
     1021 ttcaggtctc ggggcggtca gagcagagag gcatacgtaa aacaatcgac catctgcccc
     1081 ggctccacgc gcgtgacgct ctcgccgagc tcgaccacgc cgtcggtatc gaccaacgag
     1141 gataggagcc cggcgccttc gcgcggaaat ttgacggctt ccagcgtgcc gtcctcgctc
     1201 ttgcgtaagt agacgcgcac gtactcgcgg cgcgcgggct tcttgcgatg cgcgaactgc
     1261 accctgaccg gtatagccgg aggcggcgac cattgcgcgc cggcgagcgt aaaaatcgcc
     1321 ggccgcgcga cgcgggcgaa cgtcacgaag ctcgcgacgg ggttgcctgg cagcccgatg
     1381 aacggcgtgc cgtcgatgac gcccatcgcc acgggacgcc ccggcttcat cgcgatacgc
     1441 cagaagacca aggtgccgac cttctcgacg gcggccttga cgaagtcccc ttcgcccgtc
     1501 gagacgccgc ccgagctcaa gatcaggtcg ttcgaccgcg cggcggcttt gagcacctcg
     1561 gcgatctgcg ccgagtcgtc gcggcgaatg cccaaatcgc cgacgctgca gccgatgcgc
     1621 cgcagcatcg cgatcagcat caaccggttc gagtcgtaga gctgcgcgtc gcgcctggcg
     1681 gagcccggct cgacgacctc gtcgccgttc gagaacacgg cgaccttgac gcgcctgcgg
     1741 acgcggatcg acgtgaggcc catggccgcc gcggccgcga cgtcctgcgg acgcagccag
     1801 cggccggccg gcaagacgac gcgtcccaac gcgatgtcct cccccgccgg ccgcacgttg
     1861 gcgccgcgct cgaggccggc cggaagcagc acacggcccg catcgtcgag gcgggcatcc
     1921 tcttgcatga acaccgtgtc ggtgccttta ggcatcggcg cgcccgtgaa gatacgcacg
     1981 gcttcgcccg ccgcgaccgt cgcagctgcg cttgcgctgc cggcctgcac gcggcccgag
     2041 acgcggaaca gccgtgccgc gccgctcggc aagtcctcgc cgcgcacggc gtacccgtcg
     2101 acggccgagt tcgtgaacgg cggcagctgc agcgtcgcga cgaggtcgtc gccgaggacc
     2161 cgcccgtcgg cctcggcgag cgcgacggtc tccacgccct cgacatgagc gaggcgcgcc
     2221 gtgatcgcgg cgacggcgtc gtctacggag aggatttcgg cgcccgacgc gaagcagtct
     2281 tgcgatagct gcgccatgcc ggttcctaga cgatgcgatc gggccggctg tagacgttga
     2341 aacggtcggc cttcagaaag ccgacgagcg tgatgccgaa ctcgcgtgcg agctcgaccg
     2401 cgagcgatga cggcgcgccg acggccgcga cgagcggcac gccggccatc ttcgctttct
     2461 gcatgagctc gaagctcgcg cggccgctga cgatgacgcc gctttgtttc agcggcacct
     2521 cgtcatgcat cagcgcatgg ccgatcagct tgtcgagcgc gttgtgccgg ccgacgtcct
     2581 ctcgcagcgc gacgatctcg cccgtcgagt cgaacaagcc cgatgcgtgc aagccgcccg
     2641 tctgctcgaa cacggcctgc ttcgtcttca gggccgcggg cagccggccg agcgcgctgc
     2701 ccgccatgcg aaaatccgtg gcgccgacgt cgtactgccc ttgcaccgcg acggcgtcga
     2761 tcgacgcttt gccgcagacg ccgcagctcg acgtcatgta gaagttgcgc tcgaggcgct
     2821 tgaagtcgac ctcgacgccg ggcgcgagct cgacgcgcac gacgttgatg aggccgtcgg
     2881 ccgcgcgctg gccgcgcgcg acgacgcctt gcacgtcggt gcgcgcccga atgatgcctt
     2941 ccgtgaacag aaagccgacg gcgaggtcct cgtcgtgacc cggcgtgcgc atcgtgatcg
     3001 agacgggctc ctcggcgcgg ctgccgtcgc gccgcgaata accgaggcga atctcgaagg
     3061 gctcctcggt cgcgacgaga tccgggtccg cgctcgtccc gagctcgccg ccgcggaaac
     3121 ggcggacttc cagtggccgc gccgattgcc gccgaccgac cgcgtctatg tcgcccatga
     3181 cccacccacg cgccgaacga agctcgaatc gatggcacga acgttcgggc agccgagcag
     3241 cgccatgtcg cgcgcgagct cggtcttgag gatcttgagc gcctgatgca cgccggcctc
     3301 gcccccggcg ccgaggccgt agagatgcgg ccggcccgcg gaacaggcgc gcgcgccgag
     3361 cgcgagcgct ttgagaatat gcgtgccgcg gcgcacgccg ccgtcgagga tgacctcgag
     3421 ccggtcgccg gcggcgtcga cgatctcctt gagcacgtcg aacggcgccg gcgagaagtc
     3481 gagctggcgc ccgccgtggt tcgacaccat gacggcgctg gcgccgatgt cgtgagctcg
     3541 cttcgcatcc tcgccggtca tgacgccttt gatcgcgaac ggcccgcccc attgcttgat
     3601 catccactcg gcatccttcc acgtgatgct gcggtcgatc tgcgcgttca agaactggat
     3661 gatgccgccg agcttgtcgc tgccgggcgg cgggcgatgc gcgatgttcg cgacctcgat
     3721 cttcggcgtc gtcaagtgct tgtacaccca cttaggcttc atcgcgatgt cgatcaggct
     3781 catcagcgcg agcttcggcg gcaccgtcat gcccgtcacg atgtcgcgct cgcgattgcc
     3841 cgagaccgtg acgtcgaccg tgagctgcag cgcgacgtag ccggaatccc gcgcgcgctg
     3901 cacgaattcg cggctcaagc cgcggtcgcg gaacatgtag acctggaaca ccttgggacc
     3961 ggggctcagc ttcgcggcat cctcgatgtt cgtcgacgag acgctcgata atgaaaagat
     4021 cgtgccgctc gcatgcgcgg cgcgcgcggt gccgccctcg ccttcgtaat ggaacaagcg
     4081 ctgcagcgcc gtcggcgagc agaagtacgg ccattcgatt ttttggccga acagcgtcgt
     4141 ggacatgtcg atcgtcgaca cgtcgacgag cgcgcgcggc aggaactcga cgccgtcgaa
     4201 cgcggccgtg ttgcggcgca aggtccattc gtcgtcggcc gcgccgtcga tgtaatggaa
     4261 catcggcgcg ggcatgtttc gcttcgcgag cttgcgcagc tccgcgatgt tgtagcagcg
     4321 ttcgatccgg ttagagaaca cgcggcggct cccgatgccg aggagggttg tggctacgca
     4381 acggagtcgt ccgggcggcc gtgactcgct tcgtctttct cgagcaagat ctgcagcacc
     4441 ttgccgcctc cgatgggttg gctgccctcg acggacacct cgccggggcg caaccacagg
     4501 tcgagtcgag ctttcggtaa cgtcacgagt atcgcggact cggcgaacgt cg
//
LOCUS       DASSNN010000355         3018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_441586, whole genome shotgun sequence.
ACCESSION   DASSNN010000355 DASSNN010000000
VERSION     DASSNN010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441586"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..>3018
                     /locus_tag="VFL84_06520"
     CDS             173..>3018
                     /locus_tag="VFL84_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7608311.1"
                     /translation="MPGTSLDTLAARLRRELTGDVLFDTWSRGRYATDASIYQIEPLG
                     VVVPRTGEEAVRAAELAAEAGVPILPRGAGTSQCGQTVGAALVIDFSKHVNRLLEVDV
                     ANATAWVEPGIVLDDLNARLRQHGLWYPVDVSTSAQATIGGMAANNSCGARSLRYGNM
                     VHNVLELDVWLSSGETLTFGAVGDGAPQGAPRHRELVATIRGLYSRERDEIEARVPKL
                     QRRVAGYNLDLASSGAFNAAQLLVGSEGTLGLFRKVKLKLAPLPAHKALGVVHFPTFY
                     QAMDLTRHIVALEPAAVELVDRTMIELSRGIAAFRATMDKTIRGEPEAILLVEFAGDE
                     LEPQRKKLAALRELMGELGLPGSVVEIADARWQREVWEVRKAGLNIMMSMKGDGKPIS
                     FIEDCAVPLEHLAEYTDRLTRVFEKHGTRGTFYAHASVGCLHVRPVLNLRTEHGALKM
                     RAIAEEACALVKEYKGSYSGEHGDGLVRSEWIEPVYGSRLTRAFEELKRAFDPKGLMN
                     PGKIVAAPRMDDRRLFRFKPGYETAPLATGLDWSEHEVGPENRGRGFAAAVEMCNNNG
                     HCRKFDSGTMCPSYRVTRDEQHVTRGRANSLRLALSGQLGLNALASRELYETLDLCVS
                     CKGCKRECPTGVDMAAMKIEFLYHYHRTHPRSLHDRLVAHLPRYARAAARFAPLLNLR
                     DRVPGLAKLSETVAGFAAKRTLPKWRRDYFRASELGGDAGGEREVVLFADTFNTYFEP
                     DNLRAALAVLRAGGYSVRAAAAPGGRALCCGRTYLATGMIEAARAEARRVLEALAPFV
                     ARGIPVVGLEPSCILGLRDELKTLCPGAASGALARLALTFEEFLAREVEAGRWTRSFR
                     RTPYRRAVVHGHCHQKAFGAMPAVVTALKLVPELSVEVVASSCCGMAGSFGYRPEHLD
                     VSLRMAEDALLPAVRSAEAGAAIVADGTSCRHQIR"
BASE COUNT          463 a         1033 c         1043 g          479 t
ORIGIN      
        1 ccgacggggc agtccggcgg gaaatcaaga ttctaccaaa cgtgagtgtt gtgccagccg
       61 cccggcgcgg gcgccagctg tagcgcaacg gcgccagcat ttccgccgtt tcggccatgg
      121 gtgggggccc ctacaatagc cgcgcctccg cacatttaaa gatcggccgt acatgcccgg
      181 aacctccttg gatacccttg cagcacgcct tcgccgcgag ctcacgggcg acgtgctgtt
      241 cgacacctgg agccggggcc ggtatgcgac ggacgcgtcg atttaccaga tcgagccgct
      301 cggcgtcgtc gtgccgcgca ccggcgagga ggccgtccgt gcggccgagc tcgccgccga
      361 agccggcgtg ccgatcctgc cgcgcggcgc cggaacctcg caatgcggcc agaccgtcgg
      421 cgccgcgctc gtcatcgact tcagcaagca cgtgaatcgg ctgctcgagg tcgacgtcgc
      481 caatgcgacc gcgtgggtcg agcccggtat cgtgctcgac gatttgaacg cacgactgcg
      541 ccagcacggt ctctggtacc ccgtcgacgt ctcgacgtcg gcgcaagcga cgatcggcgg
      601 catggcggcg aacaacagct gcggcgcgcg ctcgctgcgc tacggcaaca tggtccacaa
      661 cgtcctcgag ctcgacgtct ggctctcgag cggcgagacg ttgactttcg gcgccgtcgg
      721 cgacggagcg ccgcaaggag cgccgcgtca tcgcgagctc gtcgcgacca tacgcggctt
      781 gtactcgcgc gagcgcgacg agatcgaagc gcgcgtgccg aagctgcaac ggcgcgtcgc
      841 cggctacaac ctcgacctcg cgtcgagcgg cgcgttcaac gccgcgcagc tgctcgtagg
      901 ctcggaaggc acgctcggct tgttccgcaa ggtcaagctc aagctcgcgc cgcttcccgc
      961 gcacaaagct ctcggcgtcg tgcacttccc gacgttctat caggcgatgg atctgacgcg
     1021 gcacatcgtc gcgctcgagc cggcggccgt cgagctcgtc gaccgcacga tgatcgagct
     1081 gtcgcgcggc atcgccgcgt ttcgcgcgac gatggacaag acgattcgcg gtgagcccga
     1141 ggcgatcctg ctcgtcgagt tcgccggcga cgagctcgag ccgcagcgga agaagctcgc
     1201 cgccttgcgc gagctcatgg gcgagctcgg gctgcccggc agcgtcgtcg agatcgccga
     1261 cgcgcgctgg cagcgcgagg tctgggaggt tcgtaaagcc ggcctcaaca tcatgatgtc
     1321 gatgaaaggc gacggtaagc cgatctcgtt catcgaggac tgcgccgtcc cgctcgagca
     1381 tctagccgaa tacacggacc gcttgacgcg cgtgttcgag aagcacggca cgcgcggcac
     1441 tttctacgcg cacgcatccg tcggctgctt gcacgtgcgg ccggtcctga acctgcgcac
     1501 ggagcatgga gcgctcaaga tgcgtgcgat cgccgaagag gcgtgcgcgc tcgtcaagga
     1561 atacaagggc tcgtactcgg gcgagcacgg cgacggactc gtgcgctcgg agtggatcga
     1621 gccggtgtac ggctcgcgct tgacgcgcgc gttcgaggaa ttgaagcgcg cattcgaccc
     1681 gaaaggtctc atgaatcccg gcaagatcgt cgcggcgccg aggatggacg accggcggct
     1741 gttccggttc aagccgggtt acgagacggc gccgcttgcg accgggctcg actggtcgga
     1801 gcacgaggtc ggccccgaga accgcggccg cggtttcgcg gcggccgtcg agatgtgcaa
     1861 caacaacggc cattgccgca aattcgactc gggaacgatg tgcccgtcgt atcgcgtcac
     1921 gcgcgacgag cagcacgtga cgcgcggccg cgcgaactct ctgcggctcg cactctcggg
     1981 ccaacttggg ttgaacgcgc tcgcgtcgcg cgagctgtac gagacgctcg acctgtgcgt
     2041 ctcgtgcaaa ggctgcaagc gcgagtgccc gaccggcgtc gacatggcgg ccatgaagat
     2101 cgagttcctg tatcactatc accggacgca tccgcgctcg ctgcacgacc ggctagtcgc
     2161 gcacctgccg cgttacgctc gggccgcggc gcgattcgcg ccgctgctga acttgcgcga
     2221 ccgcgtgccg ggtctcgcga agctgtccga gaccgtcgcg ggcttcgcgg cgaagcgcac
     2281 gctaccgaag tggcggcgcg actatttccg cgcgagcgag ctcggcggcg acgcaggcgg
     2341 cgagcgcgag gtcgtgctgt tcgccgacac cttcaatacc tacttcgagc ccgacaacct
     2401 gcgcgcggcg ctcgcggtgc ttcgggccgg cggctattcg gtgcgggcag cggccgcgcc
     2461 tggcggacgc gcgctgtgct gcggccgcac gtatctcgcg acgggcatga tcgaggccgc
     2521 gcgcgcggaa gcacggcgcg tgctcgaggc attggcgccg ttcgtcgcgc gcggcatccc
     2581 cgtcgtcggc ctcgagccgt cctgcatcct cggcctgcgg gacgagctca agacgctgtg
     2641 ccccggcgcc gcgagcgggg cgctcgctcg cctggcgctg acgttcgagg agtttctcgc
     2701 tcgcgaggtc gaagccgggc gctggacgcg cagcttccgg cgcacaccct atcgccgcgc
     2761 cgtcgtgcac ggccattgcc atcagaaggc gtttggcgcc atgccggccg tcgtgaccgc
     2821 gttgaagctc gtgccggagc tgtcggtcga ggtcgtcgcg tcgagttgct gcggcatggc
     2881 cggctcgttc ggctaccgtc ccgagcacct cgacgtctcg ttgcgcatgg ccgaagacgc
     2941 tctgctgccc gcggttcgta gcgccgaagc cggcgccgcg atcgtggctg acggcacgag
     3001 ctgccgtcac cagatccg
//
LOCUS       DASSNN010000356         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_298517, whole genome shotgun sequence.
ACCESSION   DASSNN010000356 DASSNN010000000
VERSION     DASSNN010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298517"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFL84_06525"
     CDS             <1..519
                     /locus_tag="VFL84_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HET7608312.1"
                     /translation="AEGFQHVQEKLAELWVSLETMRGLLRAAEADAAADEWGVVRPAW
                     NPLDAARNLYPRLYPRMIEIVQQLGASGLVAMPTARDLEGPLADDIRRYYQAARADAF
                     ERVPLFKLAWDVAISAFAGRQVLYERFFFGDPVRMAGSLVAAHDAEIRGYAERVRELV
                     RELRDEAFEADK"
     gene            complement(643..1053)
                     /locus_tag="VFL84_06530"
     CDS             complement(643..1053)
                     /locus_tag="VFL84_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7608313.1"
                     /translation="MRVSGWLNVLGGGLLAAVAAPVVAHHSFAAEFDINRPIKLDGVV
                     TKMEFSNPHSWLHIEVTTATGEKQQWAVEGGAPNALMRHGWNRNSVPPGTMVHVEGFQ
                     AKDRTFRASGRDITLPDGKSLFMGQGGAAAPSAE"
     gene            complement(1103..1912)
                     /locus_tag="VFL84_06535"
     CDS             complement(1103..1912)
                     /locus_tag="VFL84_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608314.1"
                     /translation="MRQRFMVSLVAALVLAAAVPVGAQAQNRPARVGGKPNINGIWQA
                     MNTAYWNLEAHAATPLNDFWRLGSIGAIPAGQSVVKEGKIPYLPEALARRDENRAGWP
                     KTDPEAKCYLPGIPRATYLPYPFQIVQGDGDIFFAYAFANANRTVHMKEHRTLDEVPT
                     DFFMGWSNGRWEGDTLVAETIANDDRTWFDRAGNYHSNQLKVTERFTPLDANRMQYEA
                     TIDDPETFSKPWTISMHLYRNTEPNAELLEFKCVEFSEHLLYNEFLKEPLP"
     gene            2082..>2758
                     /locus_tag="VFL84_06540"
     CDS             2082..>2758
                     /locus_tag="VFL84_06540"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7608315.1"
                     /translation="MLIARTLLALSIAALCSSAAVAQHKDVPFRGGIPIAPSGLADKP
                     LGDGPFDYATGEGQNIRVTVLTKALSYPFTLTFLPDGTLLITQRDGKLRVMRGGKLDP
                     NPVAGGPASFSTGTSGLPGAVHGYMDIALHPQFARNQTLYLSYTKPLADNRTTFAVTR
                     ARWTGSALADAKDIFVAEPGDGGPTPLAFGRDGMLYIATSGGNAQDGMNLGGKVLRVR
                     DDGSVPN"
BASE COUNT          448 a          930 c          901 g          479 t
ORIGIN      
        1 gccgagggct ttcagcacgt gcaagagaag ctcgcggagc tttgggtttc gctcgagacg
       61 atgcgcggcc tcctgcgcgc cgccgaagcc gacgcggcgg ccgatgagtg gggcgtcgtg
      121 cggccggcat ggaacccgct cgacgccgcg cgcaacctct acccgcgact ctatccgcgc
      181 atgatcgaga tcgtgcagca gctcggcgcc agcggcctcg tcgcgatgcc gacggcgcga
      241 gacctcgaag gcccgctcgc cgacgacatc cgccgctact accaagcggc gcgcgcggat
      301 gcgttcgagc gcgtgccgct gttcaagctc gcctgggacg tcgcgatctc ggcattcgcg
      361 ggccggcagg ttctctacga gcggttcttc ttcggcgatc cggtgcgcat ggcgggctcg
      421 ctcgtcgcgg cgcatgacgc cgagatccgc ggctacgccg agcgcgtccg cgagctcgtg
      481 cgggagcttc gcgacgaagc gttcgaagcc gacaaatagc gaccggccag cgggttacac
      541 gcggctgcta aggtattatc tgatcacgcg gctgcgccga gataggttaa aaaaaacgcc
      601 gcggctttcg tcgcggcgct tgtgctcgtg cggagctcga ggctactcgg cgctcggcgc
      661 cgctgccccg ccctggccca tgaacaacga cttgccgtcc ggcagcgtga tgtcgcggcc
      721 gctcgcgcgg aacgtgcggt ccttggcctg gaagccttcg acatggacca tcgtgccggg
      781 cggaacggag ttgcggttcc agccgtgacg catcagcgcg ttcggggcgc cgccttcgac
      841 ggcccactgc tgcttctcgc ccgtggcggt cgtgacctcg atgtgcagcc acgagtgcgg
      901 attgctgaac tccatcttcg tgacgacgcc gtcgagcttg atcggccgat tgatgtcgaa
      961 ctcggccgcg aacgaatggt gcgcgacgac gggcgcggcc actgcagcga gcaagccgcc
     1021 gccaagaacg ttcaaccaac cactgactcg cataggtcct cctagcttca aaccgattgt
     1081 ccaagcttac ccaagctttc gctcagggca gcggctcctt caagaattcg ttgtagagca
     1141 ggtgctccga gaactcgacg cacttgaact cgagcagctc cgcgttcggc tcggtgttgc
     1201 gatagaggtg catcgagatc gtccatggct tcgagaacgt ctccggatcg tcgatcgtcg
     1261 cctcgtactg catgcggttc gcgtcgagcg gcgtgaaacg ctcggtgacc ttgagctgat
     1321 tgctgtggta gttgcccgcg cgatcgaacc aggtgcgatc gtcgttggcg atggtttcgg
     1381 cgacgagcgt gtcgccctcc cagcggccgt tcgaccaacc catgaagaag tccgtcggca
     1441 cctcgtcgag cgtgcggtgc tccttcatgt gcaccgtgcg attcgcgttc gcgaacgcgt
     1501 aggcgaaaaa gatgtcgccg tcgccctgca cgatctggaa cggatacggc aaatacgtgg
     1561 cgcgcggaat gccgggcagg tagcacttgg cttcgggatc cgtcttcggc cagccggcgc
     1621 ggttctcgtc gcggcgcgcg agcgcttcgg gaaggtacgg gatcttgcct tccttgacga
     1681 cgctctgccc tgccggaatg gcgccgatcg agcccaagcg ccagaaatcg ttcagcggcg
     1741 tggcggcgtg cgcttcgagg ttccagtacg ccgtattcat ggcctgccag atgccgttga
     1801 tgttcggctt gccgccaacc cgggcggggc ggttctgcgc ctgtgcgccg acgggcactg
     1861 ccgccgccag caccagcgcc gcaaccaggg aaaccatgaa ccgctgccgc atcttgcccc
     1921 ccagaccgat tgtgattctt cgaaagccgg ccggaatcat aacgaactcg attcggcggt
     1981 tgcagccgcg aaatccaaat ttaacttgtc gcctggccgc cggcttggga ggccgcggta
     2041 gtcgacctat actgcggctt ccaactcggg ggtcactttt catgctcatc gcccgcacgc
     2101 tgctcgcgct ttcgatcgcc gcgctctgct cgtccgccgc ggtcgcccaa cacaaagacg
     2161 tgcccttccg cggcggcatc ccgatcgcgc cgtcagggtt ggccgacaag ccgctcggcg
     2221 atgggccgtt cgactacgca accggcgaag gacagaacat ccgcgtcacg gtgctgacga
     2281 aggcgctgtc gtatccgttc acgctgacgt tcctgcctga cggcacgctg ctgatcacgc
     2341 aacgcgacgg caagctgcgc gtcatgcgcg gcggcaagct cgacccgaat ccggtcgcgg
     2401 gcggcccggc ctcgttctcg accggcacgt cgggcctgcc gggcgccgtg cacggctaca
     2461 tggatatcgc gctgcacccg caattcgcgc gaaaccagac gctgtacttg agctacacga
     2521 agccgctcgc ggacaaccgc acgacgttcg ccgtgacgcg ggcgcgttgg accggcagcg
     2581 ccctcgccga cgccaaggac atcttcgtcg ccgagcccgg cgacggcggg ccgacgcccc
     2641 tcgcgttcgg ccgcgacggc atgctgtaca tcgcgaccag cggcggcaac gcgcaagacg
     2701 gcatgaacct cggcggcaag gtgctgcgcg tccgcgacga cggcagcgtg ccgaacga
//
LOCUS       DASSNN010000357         2556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1301303, whole genome shotgun sequence.
ACCESSION   DASSNN010000357 DASSNN010000000
VERSION     DASSNN010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301303"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..886
                     /locus_tag="VFL84_06545"
     CDS             230..886
                     /locus_tag="VFL84_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET7608316.1"
                     /translation="MSGLDYYRILQIQPDAPAPMVHTSYQTLVRRALRDSPGGDEIAR
                     LDAAYAVLSDPQRRAAYDLERSGRTAARAVAEPGTCLFCGATYAVGGELKRDDECGRC
                     FSPLYPADRHRLEYSGQRILGRIQVRHAISIWPKWAQETPISGEMRNVSLNGLSFTAP
                     VLFEVNQIVRIGCTELHALGRIAHAEQVTGAAHPFSAGLEFLTLRLPEGGGTFYSPKK
                     "
     gene            980..1849
                     /locus_tag="VFL84_06550"
     CDS             980..1849
                     /locus_tag="VFL84_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HET7608317.1"
                     /translation="MLRIALRRALAAALAVTAAFGAGAQSNVSSPRLYVLDGGVLKSE
                     TARYRLTDADVEEVELSIASFLIVHPRGVLLWDAGAVADHERAGPVGFEQHLVRHDGQ
                     DRFVKLAPTLTSQLQAAGYEPKDVTYLALSHYHWDHTADANMFANAQWLVPKVEHDAM
                     FGADPPGATRPETYSALKNARTTLITEPDHDVFGDGTVVIHAAPGHTEGHSVLYVKLE
                     KTGGVVLSGDLYHYPAERTLKRLPTFEVSEAETRASRDEVERFLRRTNAALWIQHDLA
                     AHRKLKKSPEYYD"
     gene            complement(1865..>2556)
                     /locus_tag="VFL84_06555"
     CDS             complement(1865..>2556)
                     /locus_tag="VFL84_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET7608318.1"
                     /translation="DPNAADPSDRIYNGAMDNASGVGMVLAIGEALRSLPQPPRRSVM
                     LLFVAAEEQGLIGSQYFAEHPSMPAGKIAANINYDSGNIWGETRDITFIGLGKSSLDA
                     VAQEVAAKQGRTVKGDQMPDRGFYYRSDQFNFAKIGVPTFYFDGGTDFIGRPAGWGVE
                     QIERYEANNYHQPSDELTPEWNFDGMVQDTRFGLLAGLIVANDDSLPQWNPGNEFEAA
                     RRAALEAAGSR"
BASE COUNT          425 a          857 c          839 g          435 t
ORIGIN      
        1 gggatcggtt cgagacgcgg acggcacggc gttggcaacg gcgctcatgg cggtctccaa
       61 tgggatatcg gtggatgggc tggtatttta tccagctcat aaccagctgc caaataacga
      121 ttttcgataa gaacggccag attcagcagc gaaaattccc gctctggcaa gcgattcctg
      181 tgaccacgtt ataagagcgc ggcgcgtccg ctttgctagc gtcaacgaaa tgagcggcct
      241 cgattactac cgaattttgc agattcagcc cgacgcgccg gcgccgatgg tccacacgag
      301 ctaccagacg ctcgtgcgcc gcgcgctgcg cgacagcccc ggcggtgacg agatcgcgcg
      361 gctcgacgcg gcgtatgccg tgctctccga cccgcagcgg cgcgccgcgt acgatctcga
      421 gcgctcgggc aggacggcgg ctcgcgccgt tgcagagccc ggcacgtgct tgttctgcgg
      481 cgcgacgtat gcggttggcg gcgagctcaa gcgcgacgac gaatgcggcc gctgctttag
      541 cccgctctat cctgcggatc gccatcggct cgaatactcg ggccagagaa tcctcgggcg
      601 tatccaggtg cgccacgcga tcagcatttg gcccaagtgg gcccaggaga caccgatctc
      661 gggcgagatg cgcaacgtgt ccctgaatgg cctctccttc accgccccgg tgctcttcga
      721 ggtgaatcag atcgtgcgca tcggttgcac ggagctgcac gcgctcgggc gcatcgcgca
      781 tgcggagcag gtcacgggcg ccgcgcatcc gttcagcgcg gggctcgagt tcttgactct
      841 gcgtctcccc gaaggcggcg gcacgttcta ctcgcccaag aagtgagcat ggcgcgcgcc
      901 tgacgaatgg cgcatcatga tcgcgcgctc aggcttcaag ccgcggcgcc ggaacacaag
      961 aaaactgccg ggggattcca tgcttcgcat tgcactgcgg cgcgcgctcg ccgccgctct
     1021 cgccgtaaca gcggctttcg gcgcgggcgc ccagagcaac gtctcgtcgc cgcgcctcta
     1081 tgtgctcgac ggcggcgttc ttaagtcgga gacggcgcgc taccgcctga ccgacgcgga
     1141 tgtcgaggag gtcgagctct cgatcgcgtc cttcctgatc gtgcatccgc gcggcgttct
     1201 gttgtgggac gcgggcgccg ttgcagatca cgagcgcgcc ggcccggtcg ggttcgagca
     1261 gcacctcgtg cggcacgacg gccaggaccg gttcgtcaag ctcgcgccga cgctgacgtc
     1321 gcagctgcag gccgcgggct acgagccgaa ggacgtcacc tatctcgcgc tgtcgcacta
     1381 tcactgggac cacacggccg acgcgaacat gttcgcgaac gcgcagtggc tcgtgccgaa
     1441 ggtcgagcac gacgcgatgt tcggcgcgga tccgcccggc gcgactcgcc ccgagacgta
     1501 ctcggcgctc aaaaacgcgc gcacgacatt gatcacggag cccgaccacg acgtgttcgg
     1561 cgacggcacg gtcgtgatac atgccgcgcc cggtcacacc gaagggcact ccgtgctcta
     1621 cgtgaagctc gagaagaccg gcggcgtcgt gctgtcgggc gatctctatc actaccccgc
     1681 cgagcgcacg ctgaaacggc tgccgacgtt cgaggtcagt gaggccgaga cgcgcgcgtc
     1741 gcgagacgag gtcgagcggt tcttgcggcg cacgaacgcg gcgctatgga tccagcacga
     1801 cctggccgcg catcgcaagc tcaagaagtc gcccgagtac tacgactgac gcttacgctg
     1861 cgcgctaacg cgagcccgcg gcctcgagcg ccgcgcggcg cgccgcttcg aactcgttgc
     1921 ccgggttcca ctgcggcagc gagtcgtcgt tcgcgacgat gagccccgcc agcaggccga
     1981 accgcgtgtc ctgaaccatg ccgtcgaagt tccattcggg cgtcagctcg tcgctcggct
     2041 ggtggtagtt gttcgcctcg tagcgctcga tctgctcgac accccaaccc gccggccggc
     2101 cgatgaagtc ggtgccgccg tcgaagtaga acgtcggcac gccgatcttg gcgaagttga
     2161 actgatccga gcggtaatag aagccgcgat ccggcatctg gtcgcccttc accgtgcgcc
     2221 cttgcttggc cgcgacttcc tgcgcgacgg cgtcgaggct cgacttgccg agaccgatga
     2281 acgtgatgtc gcgcgtctcg ccccagatgt tgccgctgtc atagttgatg ttcgcggcga
     2341 ttttgcccgc cggcatcgac ggatgctcgg cgaagtattg cgagccgatg aggccctgct
     2401 cttcggccgc gacgaacagc agcatcaccg aacggcgcgg cggctgcggc agcgagcgga
     2461 gcgcttcgcc gatcgcgagc accatgccca cacccgacgc gttgtccatc gcgccgttgt
     2521 agatgcggtc cgacggatcg gccgcgttcg gatcgc
//
LOCUS       DASSNN010000358         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1812848, whole genome shotgun sequence.
ACCESSION   DASSNN010000358 DASSNN010000000
VERSION     DASSNN010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812848"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFL84_06560"
     CDS             <1..225
                     /locus_tag="VFL84_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET7608319.1"
                     /translation="DQRTQIPGRGGQRPGTVPHWRGIFDDDERLMVAINFNMDMGDAW
                     EHADDPWYPEPMTALAYKFAVNYLIYTMTH"
     gene            complement(317..1378)
                     /locus_tag="VFL84_06565"
     CDS             complement(317..1378)
                     /locus_tag="VFL84_06565"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET7608320.1"
                     /translation="MTYMRVVASVLVVGCCLSAHAQPPQACEPSGDVSYVCGVKNPED
                     LVLVPGTAWIVSSGMADGAGFYLVDSKAGTAAVLPFTAQHDASFAGCATPPAPQSLNT
                     HGLSIRANGPGRAKLYVVGHGAREAIEVFDIDSTGARPTLTWRGCVPMPEGLAANSVA
                     SFADGGIVATVLFMPGTTFADAVVDRKPTGAVFEWSPGETAFTLVEGTQLPANNGIET
                     SADGREIYVASSGLQTIVAFSHTNPARQLRTSRPLPFTPDNVHLGPDGRLLTAGMAND
                     VPECGGPPGPQHDIEKLAACPRPTIAVAIDPATMRDTVIATTKADAKFSNATMVLTAA
                     GQAWIGTFSGDKIARTPLR"
     gene            complement(1375..1713)
                     /locus_tag="VFL84_06570"
     CDS             complement(1375..1713)
                     /locus_tag="VFL84_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608321.1"
                     /translation="MSHRVKELAEREARLQERCAAQRAIIAHELASIEARFERVDSIA
                     RVARTTLLNPIVIVGAVVALLTIGRSRGLRIVGRLWVLSTALRRLVQTIRVFRELVPK
                     QSAPIGGEPL"
     gene            complement(1710..2138)
                     /locus_tag="VFL84_06575"
     CDS             complement(1710..2138)
                     /locus_tag="VFL84_06575"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET7608322.1"
                     /translation="MEHDVDSMPPDPVEPARGPIAGLFASLAKLLATAIGIAQTRLEL
                     LTTELQEEVHRVAEIMVYAVIALLAAGVGLFLLALVVIFAFWDTHRIIASIAVTSTVF
                     LIAVVAALVLKAKIRTKPAMLNATIAELKKDRASLMRSRS"
     gene            complement(2144..2449)
                     /locus_tag="VFL84_06580"
     CDS             complement(2144..2449)
                     /locus_tag="VFL84_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HET7608323.1"
                     /translation="MAEQLSASQLIDDLTAVIRDAESLLRATASQTGDKVEEIRSRAE
                     ETVRNAKARLANVEEQALERARVFAGDADEYVRGNPWQAVAIAAGAGLLIGLLMSRR"
     gene            complement(2534..>3004)
                     /locus_tag="VFL84_06585"
     CDS             complement(2534..>3004)
                     /locus_tag="VFL84_06585"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HET7608324.1"
                     /translation="WLLLTLPLSLYSLPRWRNRVAWLAGMILVPLTFWELRGQIPAAG
                     LAVIDARVTQTIEGLIPGAPVARIRQKDLEQGIIAFAAIRAPAGLSQQVVFEWRHNGE
                     RERIEVEIHGGRADGFRAYSRKQAFPPNSTGLWTVDVLTPQAQLLERLKFVVEG"
BASE COUNT          497 a         1033 c          998 g          476 t
ORIGIN      
        1 gatcaacgca cgcagatccc gggacgcggc ggccagcggc cgggcaccgt gccgcattgg
       61 cgcggcatct tcgacgacga cgagcgcctc atggtcgcga tcaacttcaa catggacatg
      121 ggcgacgcgt gggagcacgc agacgacccg tggtacccgg agccgatgac ggcgctcgcg
      181 tacaagttcg ccgtcaacta cctcatctac acgatgacgc actgacgccg cgcgttcggc
      241 gggcgcgagc ggcgctcgag caggcgcgag cgtgcgcgcc cgctcgtgca cacgcccgcg
      301 cccggtgagc gtcgggtcag cgcagcggag tgcgcgcgat cttgtcgccg gagaacgtgc
      361 cgatccaggc ttggcccgcg gccgtcagca ccatcgtcgc gttgctgaac tttgcatcgg
      421 ccttcgtcgt cgcgatcacg gtgtcgcgca tcgtcgcggg gtcgatcgcg accgcgatcg
      481 tcgggcgcgg gcacgccgca agcttctcga tgtcgtgctg cggacccggc ggtccgccgc
      541 actccggcac gtcgttggcc atgcccgccg tcagcaaccg cccatcgggc cccaagtgca
      601 cgttgtcggg agtgaacggc agcggccggc tcgtccgcaa ttgccgcgcc ggattggtgt
      661 gcgagaacgc gacgatcgtc tgcaggcccg acgacgcgac gtagatctcg cggccgtctg
      721 ccgaagtctc gatgccgttg ttggccggca gctgcgtgcc ctcgaccagc gtgaacgcgg
      781 tctccccagg cgaccactcg aacacggcgc cggtcggctt gcggtccacg accgcatccg
      841 cgaaggtcgt gccgggcatg aacagcaccg tcgccacgat gccgccgtcg gcgaacgacg
      901 cgacactgtt cgcggcgagc ccttcgggca tcggcacgca gccccgccac gtcaacgtcg
      961 ggcgtgcacc cgtcgaatcg atgtcgaaca cttcgatcgc ttcgcgcgcg ccgtgcccca
     1021 cgacgtagag cttcgcgcgg ccgggcccgt tcgcgcgaat gctgagcccg tgcgtgttca
     1081 gcgattgcgg agccggcgga gttgcgcagc ccgcgaagga ggcatcgtgc tgcgccgtga
     1141 acggcaatac cgcggccgtg ccggctttcg agtccacgag gtagaagccc gcgccgtccg
     1201 ccatgccgct cgagacgatc catgccgtgc ccggaaccag cacgagatcc tcggggttct
     1261 tgacgccgca gacgtaagag acgtcgccgc tcggctcgca agcttgcggc ggctgcgcgt
     1321 gcgcggacaa gcagcagcca acgaccagga cggacgcgac aacacgcatg taagtcataa
     1381 cggttccccg ccgatcggcg cgctttgttt gggcactagc tcacgaaaaa ctctgatcgt
     1441 ttgcacgagc cggcgcaagg cggtgctcaa gacccacaag cggccgacga tacgcagccc
     1501 tcgcgaacgg ccgatcgtga gcagcgcgac gacggcgccg acgatgacga tggggttcag
     1561 cagcgtcgtt cgcgcgacgc gcgcgatgct gtcgacgcgc tcgaagcgcg cctcgatgct
     1621 cgcaagctcg tgcgcgatga tcgcgcgttg cgccgcgcag cgttcctgca agcgcgcttc
     1681 ccgctcggcg agctccttga ctcgatggct cacgaccggc tccgcatgag actcgcgcgg
     1741 tctttcttga gctcggcaat cgtcgcattg agcatcgccg gcttcgtgcg aatcttcgct
     1801 ttgagaacga gcgccgcgac gacggcgatc aggaaaaccg tgctcgtgac cgcgatcgac
     1861 gcgatgattc gatgcgtgtc ccaaaacgcg aagatgacga cgagcgcgag gagaaacagc
     1921 ccgactccgg ccgcaagcag cgcgatgacc gcgtagacca tgatctccgc gacgcgatgg
     1981 acctcctctt gaagctccgt cgtgagcagc tcgagccgcg tttgcgcgat gccgatggcc
     2041 gtcgcgagca gcttcgcgag cgaggcgaac aaccccgcga tcgggccgcg tgccggctcg
     2101 accgggtccg gcggcatgct gtcgacgtcg tgctccacgc cgatcagcgg cggctcatga
     2161 gcaggccgat caagagcccg gcaccggccg caatcgccac ggcctgccac gggttgccgc
     2221 gcacgtattc gtcggcgtcg ccggcgaaca cgcgggcccg ctcgagggcc tgctcttcga
     2281 cgttcgcgag ccgcgctttc gcattgcgta ccgtttcctc ggcgcgcgac ctgatctctt
     2341 cgactttgtc gccggtttgc gaggccgtcg cgcgcagcag gctctccgca tcgcggatca
     2401 cggctgtcaa atcgtcaatc agctggctgg cggagagttg ttcggccatg ggcgtcgact
     2461 ccagtgcaag gacacgttta tagagatggt atgtctcgga tagcgcgcgt cacaaggtgc
     2521 ctttcggccg ccttcagcct tccacgacga acttgagcct ttcgagcagc tgcgcctgcg
     2581 gcgtcaacac gtcgacggtc caaagcccgg tcgagttcgg cgggaacgcc tgcttgcgcg
     2641 agtaggcgcg gaaaccgtcg gccctgccgc cgtgaatctc gacttcgatc cgctcgcgct
     2701 cgccgttgtg ccgccattcg aacacgactt gctgcgacaa cccggccgga gcgcgaatcg
     2761 cggcgaacgc aatgattccc tgctcgaggt ccttttgccg gatgcgcgcg acgggcgcgc
     2821 cggggatcaa cccctcgatc gtttgcgtca cgcgcgcgtc gatgacggca agaccggccg
     2881 cgggtatctg gccgcgcaac tcccagaacg tgagcggcac gaggatcatt ccggcaagcc
     2941 atgcgacgcg gttgcgccag cgcggcagcg agtacagcga gagcggcaac gtgagcagca
     3001 gcca
//
LOCUS       DASSNN010000359         5751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1683639, whole genome shotgun sequence.
ACCESSION   DASSNN010000359 DASSNN010000000
VERSION     DASSNN010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683639"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="VFL84_06590"
     CDS             <1..174
                     /locus_tag="VFL84_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HET7608325.1"
                     /translation="QTHVQRYMRPLLERIQKREIDPRFVITHEMKLDDAPNGYKIFND
                     KLDDCEKIVLKPH"
     gene            complement(176..1072)
                     /locus_tag="VFL84_06595"
     CDS             complement(176..1072)
                     /locus_tag="VFL84_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7608326.1"
                     /translation="MAKRDLAQSVIVLTGASSGFGKGAALELARAGAKVVLAARRDDL
                     LDEVANECSAEGGDALACPTDVSKKDDVERLCSTALETFGRIDVWVNNAGVGAIGRFE
                     RVPLDVHEQVIATNLYGTLYGSYFAYRQFLAQGHGVLINIASELGFGTVPYYSSYVAA
                     KHGVVGLSDSLRQEVKQNRIERVHVCTIMPTAHDTPFFDHVANYTGHEVTPPKPLHDP
                     QAVVDTIVRVARDPEDKEIVGADGIAKLVLTNVLPGVAEKVLAKQMHKTQMEKPPPTP
                     DSPGAVRAPMREGTGVSAGRRD"
     gene            complement(1136..1930)
                     /locus_tag="VFL84_06600"
     CDS             complement(1136..1930)
                     /locus_tag="VFL84_06600"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET7608327.1"
                     /translation="MIGALGLSKAVIDAWRGETVRLAGADLQVPGTRRIRLSVVAGNL
                     RMHRLLDRFVRPGATVVDVGANIGYNAIHAALLAGQPGRVYAVEPTPDNLAVLRANVA
                     AASLANVVVAPVAAGRSAGTRDFFVRGARSAVNSLFAESCYAAVTEVLQVPVMPLDDL
                     VDGGADVVKIDVEGAELDVLEGMPRLLRARPAALIVEWHPTLQSMAGYAPDALPRWLL
                     TRGFRVRAVSHATERSLAAADIGHVTQRLLRLQRPVELLAAPPPFR"
     gene            1991..3172
                     /locus_tag="VFL84_06605"
     CDS             1991..3172
                     /locus_tag="VFL84_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HET7608328.1"
                     /translation="MRRTTADSKLLATCRSRAVELLRNNSTPAGVLAAAPTARATERG
                     YTAIFGRDAAICALGMAVSGDRALERASVAGLETLAQYQAPNGQIPKFVTPDRREADF
                     WYLGCIDATLWWLIALALLDGPLAHRGFKPGLLRRYRRQVALALQWLGAQEHQRFYLL
                     QQNEASDWADIMPRSGFVLYTNALWYRVKRLFGLPNIAETRQSFNGLFHPFSARIFEY
                     RRGRLLNEYVLKRARNRDLYLSFVNFSFFGDEGDVFGNVLAVLCGALSKGAARRTLDA
                     LLELGVHDPYPVRAVVRPIRKQSALWRPYMARHRQNSAWQYHNGGVWPVVGGFFIAAL
                     ARCGRTQQAREELVKLARACELDDWAFAEWLHGRSAVPRGMAGQSWNAAAFLFAAHAA
                     R"
     gene            complement(3169..5169)
                     /locus_tag="VFL84_06610"
     CDS             complement(3169..5169)
                     /locus_tag="VFL84_06610"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HET7608329.1"
                     /translation="MKTASTTVLANAALLVAYFAAAKFGLSFFALIHPSASPVWLPTG
                     IALAGLLLGGLRLVPALFAGAFLANVTTAGTVASSLGVAVGNTLEAVLAATLVERFAG
                     GRNAFTSPGRILKFMVLGALLSPMVSATIGVFSLRLGGVATDAPGGAVWFTWWLGDAA
                     GAVLVTPLIVLWWTTDWDRVGRRAGEAALMITAIGVVGAATFFHPYLSQYPLAFLCLA
                     PLVWGALRFGPREIATAIVVLAVFATAATAMGLGQFARLATNESLLVLQGFLVVISMT
                     ALPMAALTVERRAALERERAARAEADAASRAKDEFLAILSHELRNPLAAISMAAAVLD
                     TGANSRDSSARLVASIRRQAQHLARLIDDLLDMSRMTANKLTLRKEPLELGDAVRRCV
                     EAFSTTRGLAPGRIELALDAVWIDADSVRVTQVVENLLGNALKHTPPGKRIGVTVRDI
                     GAVAELCVEDEGFGIRPELLPNVFDPFTQGRQGLDRSAGGLGLGLTLVRRLTELHGGT
                     VEAQSRGIDRGSAFTLRFPTRAAPAGAVAAASDQARPERPRRLLVVEDNVDARQTLRL
                     LLETLGHEVHEAADGEAGVAAALERRPDLVFVDIGLPRLDGYQVARRLREAKLGVRLV
                     ALTGYGRDDDVKRAREAGFDEHLLKPATLEQLRAAIDALAIA"
     gene            5449..>5751
                     /locus_tag="VFL84_06615"
     CDS             5449..>5751
                     /locus_tag="VFL84_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HET7608330.1"
                     /translation="MTASHKWISLLAATAAVSLFSLSANAAERIGGSDAFQRTVKTWD
                     LNLAKSEDVQTLNARVRDAAHAVCLAEARRYWASTRRNAPIGWRERCVDDAVEAAVR"
BASE COUNT          931 a         1996 c         1932 g          892 t
ORIGIN      
        1 cagacgcacg tgcagcgcta catgcgcccg ctgctcgagc gcatccagaa gcgcgagatc
       61 gacccgcgct tcgtcatcac gcacgagatg aagctcgacg acgcgccgaa cggctacaag
      121 atcttcaacg acaaactcga cgactgcgag aagatcgtcc tgaagcccca ctagattagt
      181 cgcggcggcc ggcgctgacc cccgtgcctt cgcgcatcgg ggcgcggacc gcgcccgggg
      241 aatccggcgt cggcggcggc ttctccatct gcgtcttgtg catctgcttg gcgaggacct
      301 tctcggccac gccgggcagc acgttcgtca gcacgagctt cgcgatgccg tcggcgccga
      361 cgatctcctt gtcctcgggg tcgcgggcga cgcgcacgat cgtgtccacg accgcttgcg
      421 gatcgtgcag cggcttcggc ggcgtcacct cgtggcccgt gtagttcgcg acgtggtcga
      481 agaatggcgt gtcgtgcgcg gtcggcatga tcgtgcagac gtgcacgcgc tcgatgcgat
      541 tctgcttgac ctcttggcgc agcgagtccg agagcccgac gacaccgtgc ttggcggcca
      601 cgtacgacga gtaatacgga acggtgccga agccgagctc cgacgcgatg ttgatgagca
      661 cgccgtgtcc ttgagccagg aactgacggt acgcgaagta actgccgtag agtgtgccgt
      721 agaggttcgt ggcgatgacc tgctcgtgca cgtcgagcgg cacgcgctcg aaccggccga
      781 tcgcgccgac gcccgcgttg ttgacccaga cgtcgatgcg gccgaatgtc tcgagcgccg
      841 tcgagcagag ccgctcgacg tcgtccttct tgctgacgtc ggtcgggcac gccagcgcgt
      901 cgccgccctc ggcgctgcat tcgttcgcga cctcgtcgag cagatcgtcg cggcgcgccg
      961 cgagcacgac ctttgcgccg gctcgcgcga gctcgagggc ggcccccttg ccgaagccgc
     1021 tcgaagcgcc ggtcagcacg atgacgcttt gcgccagatc tctctttgcc atgagcactc
     1081 cttgcctcga ggttgcgcta tcgcggcggc ttgcgatgca aaggccatgc cgcgctcagc
     1141 ggaacggcgg cggcgccgcg agcagctcga cggggcgttg cagccgcagt agccgctgcg
     1201 tcacatggcc gatatcggcg gccgcgagcg agcgctcggt cgcatgcgag acagcgcgga
     1261 ccctgaagcc gcgagtgagc agccagcgcg gcagcgcatc gggcgcataa ccggccatgc
     1321 tctgcagcgt aggatgccac tcgacgatca gcgctgcggg ccgagcgcgg agcagccgcg
     1381 gcatcccttc gagcacgtcc agctcggccc cctcgacgtc gatcttgacg acgtcggccc
     1441 cgccgtcgac gaggtcgtcc aacggcatga cgggcacttg cagcacctcg gtcacggccg
     1501 cgtagcagct ctcggcgaac aggctgttga cggcgctgcg cgcgccacgc acgaagaagt
     1561 cgcgcgtgcc ggccgacctg cccgccgcca ccggcgccac gacgacgttc gcgagactcg
     1621 cggccgcgac gttcgctcgc agcacggcga ggttgtcggg cgtcggctcg acggcgtaga
     1681 cgcggcccgg ctgcccggcg agcagtgcgg catgaatcgc gttgtagccg atgttggcgc
     1741 cgacatcgac gacggtcgcg ccgggccgga cgaagcggtc gagcagccga tgcatgcgca
     1801 gattgccggc gacgacggac agcctgatgc gccgcgtgcc cgggacttgc aggtcggcgc
     1861 cggcgaggcg caccgtctcg ccgcgccacg cgtcgatgac ggccttcgac aacccgaggg
     1921 ctccgatcat ggccattcga agcaagcgcc gtgccatcga ggcactaaag gaaggctaga
     1981 atcgaccgcg atgcgccgaa ccactgccga ttcaaagctg ctcgcgacgt gccgttcccg
     2041 cgccgtcgag ctcctgagga acaactcgac gccggccggc gttctggccg cggccccgac
     2101 ggcgcgcgcg acggagcgcg gctacacggc catcttcggc cgcgacgccg cgatctgcgc
     2161 gctcggcatg gccgtctcgg gagaccgcgc gctcgagcga gcttccgtcg cgggcctcga
     2221 gacgctcgcg caatatcagg cgccaaacgg ccagatcccg aaattcgtca cgcccgatcg
     2281 ccgcgaggcg gatttctggt acctgggctg catcgacgcg acgctttggt ggctcatcgc
     2341 gctcgcgttg ctcgacggac ccctcgcgca ccgcggcttc aagcccggct tgctgcgccg
     2401 ctatcgaaga caggtcgcgc tcgcgctgca atggctcggc gcgcaggagc accaacgctt
     2461 ctatctactg cagcagaatg aggccagcga ctgggcggac atcatgccgc gctccggctt
     2521 cgtgctgtat acgaacgcgc tctggtaccg cgtcaagcgg ctgttcggcc tgcccaacat
     2581 tgccgagacg cgccagagct tcaacggcct gttccatccg ttctcggccc gcatcttcga
     2641 gtatcgtcgc ggtcgcctgc tgaacgagta cgtgctgaag cgagcgcgca accgcgacct
     2701 gtacttgagc ttcgtgaact tctcgttctt cggcgacgag ggcgacgtgt tcggcaacgt
     2761 gctggccgtg ctctgcggcg cgttgagcaa aggcgccgcg cggcgcacgc tcgatgcgct
     2821 gctcgagctc ggcgtccacg acccttaccc cgtgcgcgcg gtcgtgcggc cgatccgcaa
     2881 gcagagcgcg ctgtggcgtc cgtacatggc gcggcaccgc cagaactcgg cgtggcaata
     2941 ccataacggc ggcgtctggc cggtggtggg cggatttttc atcgcggcgc tcgcgcgctg
     3001 cgggcgcacg cagcaagcgc gcgaggagct cgtgaagcta gcgcgcgcgt gcgagctcga
     3061 cgactgggcg ttcgcggaat ggctgcacgg ccgaagcgcg gtgccgcgcg gaatggcggg
     3121 ccagtcgtgg aatgcggcgg cgttcctgtt cgccgcgcac gccgcgcgtt aggcgatggc
     3181 cagcgcgtcg atggccgcgc gcagctgctc gagcgtggcc ggtttcagca gatgctcatc
     3241 gaagccggct tcgcgcgcac gcttcacgtc gtcgtcgcgg ccgtagcccg tcaatgccac
     3301 gagacgcacg ccgagcttcg cctcgcgaag ccggcgcgcg acttgataac cgtcgagccg
     3361 cggcaggccg atgtcgacga acacgagatc gggccggcgc tcgagcgctg ccgcgacgcc
     3421 cgcctcgccg tcggcagcct cgtgcacctc gtggccgagc gtttcgagca gcaagcgcag
     3481 cgtttgccga gcgtcgacgt tgtcctcgac tacgagcagt cgcctcggcc gctcggggcg
     3541 cgcctggtcg ctcgcggccg caacggctcc ggccggggcc gcccgggtcg ggaagcgcag
     3601 cgtaaaagcg ctgccgcggt cgatgccgcg gctctgcgcc tcgaccgtgc cgccgtgcag
     3661 ctcggtgaga cggcggacga gcgtcagccc gaggccgagc cctcccgcgc tgcgatccaa
     3721 tccttgccga ccttgcgtga acggatcgaa tacgttcggc agcagctccg gtcgaatccc
     3781 gaacccttcg tcctcgacgc acagctccgc gaccgcgccg atgtcgcgta cggtgacgcc
     3841 gatgcgcttg ccgggcggcg tgtgcttcag cgcgttgccg agcaaatttt ccacgacttg
     3901 cgtgacacgc acggagtcgg cgtcgatcca cacggcatcg agcgcgagct cgatgcgtcc
     3961 cggcgcgagc ccgcgcgtcg tggaaaaagc ttcgacgcag cgccgcaccg cgtcgccgag
     4021 ctcgagcggc tccttgcgca gggtcaactt gttcgccgtc atgcggctca tgtcgagcag
     4081 atcgtcgatc agccgcgcga gatgctgcgc ttgacggcgg atgctcgcga cgagccgcgc
     4141 gctgctgtcg cgcgaattgg cacccgtatc gagcacggcc gcagccatgc tgatcgccgc
     4201 cagcgggttg cgcagctcgt ggctcaaaat ggcgagaaac tcgtccttcg cgcgcgacgc
     4261 cgcgtcggcc tcggcgcggg ccgcgcgctc acgctcgagc gccgcgcggc gctcgacggt
     4321 gagcgcggcc atcggcaagg ccgtcatcga gatcacgacg agaaacccct gcagcacgag
     4381 caatgattca ttcgtcgcca agcgtgcgaa ctggcctagc cccatcgccg tggccgccgt
     4441 cgcaaagacc gcgagcacga caatcgccgt cgcgatctcg cgggggccga agcgcagcgc
     4501 gccccacacg agcggcgcga gacacaggaa cgcgagcggg tattgcgaca agtacgggtg
     4561 aaagaacgtc gccgcaccga cgacgccgat agccgtgatc atcagagccg cttcgccggc
     4621 cctgcggccc actcggtccc agtcggtcgt ccaccacagc acgatcaatg gggtgacgag
     4681 cacggcgccg gccgcatcgc cgagccacca agtgaaccag acggcgccgc ccggcgcgtc
     4741 cgtcgcaaca ccgccgagcc tcaagctgaa cacgccgatc gtcgcgctga ccatcgggct
     4801 cagcaacgcg ccgagaacca tgaatttcag gatccgcccc gggctcgtga acgcgttgcg
     4861 cccgcccgcg aaccgctcga cgagcgtcgc ggcgagcacc gcttcgagcg tgttgccgac
     4921 cgcgactccg agcgagctcg ccaccgtgcc ggccgtcgtc acgttggcga ggaacgcgcc
     4981 ggcgaatagc gcaggcacga gccgcaggcc tccgagcagc agcccggcga gcgcgatgcc
     5041 cgtcggcaac cagaccggcg aagcgctcgg atgaatcagc gcgaagaacg acaagccgaa
     5101 cttggcggcg gcgaagtagg cgaccaacaa cgccgcgttc gcgagaacag tggtggacgc
     5161 tgtcttcatt gttcttccac gcggcgagaa tacatcggat cgctcctgct ttcccgcaaa
     5221 tgggattcgt cgtcgcgcgc gcagctttcg tgaacgaccg ttaacgtcga tcccgcgatc
     5281 gggatacagg cgtgccgcgc gcgcgacgaa ataaacgata gttaatgttg cggtttcagc
     5341 aacgtcctgt tttttctcgc cgttgttacg gtagcgggcg tggcatgtaa cttgatctgg
     5401 catgaagcgt ctctcgtacg cctctccccc caacatgagg atgcttcaat gactgcttcc
     5461 cacaaatgga tttctcttct agcggccacc gcagcggtgt cgcttttctc gttgtcggcg
     5521 aatgcagcgg agcgcatcgg cggctccgac gccttccaga gaaccgtgaa gacctgggat
     5581 ctcaacctcg cgaagtccga ggacgtgcag acgctgaacg cgcgcgttcg tgacgccgcg
     5641 cacgccgttt gtctcgcgga agcgcgccgg tactgggcca gcacgcgccg caacgcgccg
     5701 atcggctggc gcgagcgttg cgtcgacgac gccgtggaag cggccgtgcg c
//
LOCUS       DASSNN010000360         2981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_65863, whole genome shotgun sequence.
ACCESSION   DASSNN010000360 DASSNN010000000
VERSION     DASSNN010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65863"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /gene="dapA"
                     /locus_tag="VFL84_06620"
     CDS             complement(<1..875)
                     /gene="dapA"
                     /locus_tag="VFL84_06620"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET7608331.1"
                     /translation="MPQRRHALAALRGSIPPLITPFKRGEVDYDRYAQLVELQVKEGS
                     HGILVNGTTSEPSTLTTEERNRLVTLAVKTTAGRVPVVAATGSQSLAESRVLTEHAIE
                     AGADSLLIVTPYYIRPPQRGLVAYYLELARGHEVPWMIYHIPGRTAVSVTLDTLKACK
                     DGSPHFVGIKHAVNDLGFASECLHEFGRDFKVFVGLEELSFPMMAVGACGLMNAVGNL
                     KPKVLAQMCEAVFAGDLATGQRLHDQLLEINKAVFFDTNPIPMKYMMKKLGLLENNEH
                     RLPMMSATPELAAKL"
     gene            1075..1476
                     /locus_tag="VFL84_06625"
     CDS             1075..1476
                     /locus_tag="VFL84_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016978424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HET7608332.1"
                     /translation="MPHLIVEYSANIEDQIALDALLDKLHTCALGTGVFPIGGLRVRA
                     HRADAYRIADKSPDNGFVHMTALIGHGRPLDVQQRAAEELFAVLTTHLEPLFAKSPLA
                     LSLNVQEFHPVLNFKKNNLHEYVKRRGGPKT"
     gene            1485..>2981
                     /gene="hpaE"
                     /locus_tag="VFL84_06630"
     CDS             1485..>2981
                     /gene="hpaE"
                     /locus_tag="VFL84_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013557033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HET7608333.1"
                     /translation="MSATLERNISNAEKYLERFRSRGVLNFIDGAPRAAQSGRTFETH
                     SPIDGTLLAAVAAGDAADVAAAAEAAQRAFLKWRDVPGGERQKLLHGIADAIESRAEE
                     IALLESLDTGQPIRFMAQAAKRGAENFRFFADKAPEADRGLSLPAREHVNYTLRQPLG
                     PVAAITPWNTPFMLSTWKIAPALAAGCTVVHKPAEWSPVTAALLAEIATEAGLPAGVL
                     NTVQGLGETAGKAATEHPLIRAVAFVGESATGSAIMAQAAPTLKRLHFELGGKNPVVV
                     FDDADLDRALDAVVFMIYSLNGERCTSSSRLLVQKSIEEELTERLVERVRKIKVGHPL
                     DPATELGPLIHPNHVEKVLGYFGVARGEGAAIPTGGAAAAQGGNYVQPTVLAGAHNAM
                     RVAQEEIFGPVLTVIPFADEKEALALANDVRYGLAGYLWTNDVGRAHRFARGLEAGMV
                     WVNSENNRHLPTPFGGMKASGIGRDGGDYSFDFYMETKNVCVAHGTHRV"
BASE COUNT          507 a          971 c         1001 g          502 t
ORIGIN      
        1 tcgagctttg ccgcgagctc cggcgtcgcg ctcatcatcg gcaggcggtg ctcgttgttc
       61 tcgagcaaac cgagcttttt catcatgtat ttcatcggga tcggattcgt gtcgaagaac
      121 acggccttgt tgatctcgag cagctggtcg tgcaggcgct ggccggtggc gagatcgccc
      181 gcgaacacgg cttcgcacat ctgcgcgagc accttcggct tcagattgcc gacggcgttc
      241 atcagcccgc aagcgccgac cgccatcatc gggaagctca gctcctcgag cccgacgaac
      301 actttgaagt cgcggccgaa ttcgtgcagg cattcggatg cgaacccgag atcgttgacg
      361 gcgtgcttga tgccgacgaa gtgcggcgag ccgtctttgc aggctttcag cgtgtcgagc
      421 gtgacgctga ccgcggtgcg gcccgggatg tgatagatca tccacggcac ctcgtggcct
      481 cgcgcgagct cgagatagta cgcgacgagg ccgcgctgcg gcgggcggat gtagtacggc
      541 gtgacgatca agagcgagtc ggcgccggcc tcgatcgcgt gctccgtcag cacgcggctc
      601 tccgcgagcg actgcgagcc ggtcgcggcg acgacgggca cgcggccggc tgtcgttttc
      661 actgcgagcg tgacgagccg gttgcgctcc tcggtcgtca gcgtcgaagg ctcggacgtc
      721 gtgccgttga cgagaattcc gtgcgagcct tctttgactt ggagctcgac gagctgcgcg
      781 tagcggtcgt agtcgacctc gccgcgcttg aacggcgtga tgagcggagg aatcgagccg
      841 cgcagcgcgg cgagagcatg tcgtctttgc ggcatttcta tctcaagcgc agcggtgacc
      901 gcccaaggga cagctcataa aaggggacag atctattttc cgcgaaaata aatctgtccc
      961 ctttttggaa aaatagatct gtcccctttt tgcgctaaat tgacagtcgc gagaggcgtc
     1021 aattaccgtg tcggatcgag ctcgagttta cgttaagccg cagccggatt caccttgccg
     1081 cacctgatcg tcgagtattc cgcgaacatc gaggatcaga tcgccctcga cgcgctgctc
     1141 gacaagctgc acacgtgcgc cctcggcacg ggcgtgtttc cgatcggcgg cttgcgcgtt
     1201 cgcgcgcata gggccgacgc ttaccgcatc gccgacaagt cgcccgacaa cggcttcgtg
     1261 cacatgacgg cgctgatcgg ccacggccgg ccgctcgacg tgcagcagcg cgcggccgag
     1321 gagctgttcg cggtgctgac gacgcacctc gagccgctgt tcgcgaagag cccgctcgcg
     1381 ctgtcgctga acgtgcagga atttcatccc gtgttgaatt tcaagaagaa caacctgcac
     1441 gagtacgtga agcggcgcgg cggccccaaa acatgagtgg cgtcatgagc gcaacgctcg
     1501 agaggaacat ctctaacgcc gagaagtatc tcgagcggtt tcgctcgcgc ggcgtgctca
     1561 acttcatcga cggcgccccg cgtgcggcgc agtccggccg cacgttcgag acgcactcgc
     1621 cgatcgacgg cacgctgctc gcagccgtcg ccgccggcga cgcggccgac gtcgcggccg
     1681 cggccgaggc cgcgcagcgt gcgtttctca agtggcgcga cgtgcccggc ggcgagcggc
     1741 agaagctgct gcacggcatc gccgacgcga tcgagtcgcg ggccgaggag atcgcgctgc
     1801 tcgaatcgct cgacacgggc cagccgatcc gcttcatggc gcaagccgcg aagcgcggcg
     1861 ccgagaattt tcgcttcttc gccgacaagg cgcccgaagc cgatcgcggc ttgtcgctgc
     1921 cggctcgcga gcacgtcaac tacacgctgc gccagccgct cggccccgtc gccgcgatca
     1981 cgccgtggaa cacgccgttc atgctgtcga cgtggaagat cgcgccggcg ctcgcggccg
     2041 ggtgcaccgt cgtgcacaag ccggccgagt ggagccccgt cacggcggcg ctgctcgccg
     2101 agatcgcgac ggaggccggg ttgccggccg gcgtgctgaa caccgtgcaa gggctcggcg
     2161 agacggcggg caaggccgcg accgagcatc cgctgatccg cgccgtcgcg ttcgtgggcg
     2221 aatcggccac gggcagcgcg atcatggcgc aggccgcgcc gacgctgaag cggttgcact
     2281 tcgagctcgg cggcaagaat cccgtcgtcg tgttcgacga cgccgacctc gaccgcgcgc
     2341 tcgacgccgt cgtgttcatg atctatagct tgaacggcga gcgctgcacg tcgtcgagcc
     2401 ggctgctcgt gcagaagtcg atcgaggaag agctcacgga gcggctcgtc gaacgcgtgc
     2461 gcaagatcaa ggtcgggcat ccgctcgatc ccgccacgga gctcgggccg ctgatccatc
     2521 cgaatcacgt cgagaaggtg ctcggctact tcggcgtggc gcgcggcgaa ggcgccgcga
     2581 taccgacagg cggcgccgcg gccgcgcaag gcggcaacta cgtacagccg accgtgctcg
     2641 cgggcgcgca caatgccatg cgtgtcgcgc aggaggagat cttcgggccc gtgctcacgg
     2701 taattccttt cgccgacgag aaggaagcgc tcgcgctcgc gaacgacgtt cgctacggct
     2761 tggccggcta cctctggacc aacgacgtcg gccgcgcgca ccggttcgcg cgcgggctcg
     2821 aggccggcat ggtctgggtc aactccgaga acaatcgcca tttgccgacg ccgttcggcg
     2881 gcatgaaggc gagcggcatc ggccgcgacg gcggcgacta cagcttcgac ttctacatgg
     2941 agacgaagaa cgtttgcgtg gcacacggga ctcaccgcgt g
//
LOCUS       DASSNN010000361         3644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1448420, whole genome shotgun sequence.
ACCESSION   DASSNN010000361 DASSNN010000000
VERSION     DASSNN010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448420"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..2915
                     /locus_tag="VFL84_06635"
     CDS             159..2915
                     /locus_tag="VFL84_06635"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7608334.1"
                     /translation="MKLAVRACALAFALLFTAVVTAQPSLPQGITRVTTVEGITEYRL
                     ANGLQVLLFRDPSKATITVNMTYLVGSVDESYGETGMAHLLEHLMFKGSKKFPDILAA
                     IRSHGAQFNGTTSWDRTNYFETFDASDANLEWALDLESDRMINAFVAQEDLDTEMTVV
                     RNEFEQGENSPTGVLFERVMSAAYLWHGYGNSPIGSRSDIENVPIPRLRAFYERHYQP
                     DNAVLVIAGRFEEQRALELIAKYFSSIPRPQRELTDNYTVEPTQDGEREVVVRRVGDV
                     QILMAGYHVPAGSHEDFVPLQIAAEVLGDVPSGRLYKALVEPGLASQIAVQEMQLRDP
                     GMIVFFALVPKEKSLEAAREAMIATIDALAANPVTTEEVERIRNQAMSSFEQQMNNSQ
                     GIAIQLSSWAAIGDWRMLFLDRDRVRTASAESVQSAALKYFKPSNRTVGLFVPGDPDR
                     SEIPARPDVTAMLQGYKGDQARADGEAFDPTPANIDARTTRVDLPGGVKLVMLPKETR
                     GDAVNALIRLNYGDEKSLTGPGRLAGVTTQMLMRGTRAKSRQDIQDELARLQSQLNVG
                     GGAGLASANIQSTRANLIEVLTLAIEVLREPAFPDAELATLKQQTITGLEASRSEPQA
                     IVSRAYARHWARNYKPDDPRYVTTIDEDLAFINGVRTAQLRDFHKDFFGASNAEVVVI
                     GDFDPEQVRALIASRLDGWKSPKPFSDVLNRYADLAHDAISQDFNTPDKENAMFLAGM
                     RIEMRDSHPDYPAMLFGNYILGSGPASRLFGRIRGKEGLSYGVGSSFGASPRSDGASF
                     TVNAIAAPQNAAKVEASFRDELATVLREGYTQEEFDAAKVSWAQGRQVSRAQDAGLVG
                     TLGLWTHVGRTMAFDADLEAKVAALTVEQVRDAMNRHLDVNKMTFMKGGDFESVSAGA
                     GGAP"
     gene            3018..>3644
                     /locus_tag="VFL84_06640"
     CDS             3018..>3644
                     /locus_tag="VFL84_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7608335.1"
                     /translation="MNRPRSSRQHYDVFRRDYREGTLDDKLATVDKQPVKAPAVKARR
                     EYLKDYLRWLWPERWTVAAVFALAVTTAGLDMISPLFMRFITDHVLLDTAAATADRFR
                     LLNLAGGLFLMLITVSAGLNIARDYRQRLVNTRVMRSLRRSLFERLLRLPLSTLWDMK
                     TGGILSRLSGDVDSTTGLLQMAIVSPSISVIRLLIAMTVLFALNWQLAL"
BASE COUNT          650 a         1223 c         1193 g          578 t
ORIGIN      
        1 gcatcgtagt ccggcggcat ccgccccggc aatggagccg catcgaaccg ccttgccgat
       61 caaggggttc gccgtgccgc cgaactagag ccgcgccatg cagtcgaact cgcgtcccgc
      121 atggtaagtt tgcaggccaa caacaacggg ggatgcccat gaaactcgcg gttcgcgctt
      181 gcgcgctcgc gttcgccctg ctcttcaccg ccgtcgtcac cgctcagccg agcctgccgc
      241 aaggcatcac tcgcgtgacg accgtcgagg gcatcaccga gtaccggctc gccaacggcc
      301 tgcaagtgtt gctgtttcgc gatccgtcga aggcgacgat caccgtcaac atgacgtacc
      361 tcgtcggctc cgtcgacgag agctatggcg agacgggcat ggcgcacttg ctcgagcacc
      421 tgatgttcaa gggctcgaag aaattccccg acattctcgc cgcgattcgc agccacggcg
      481 cgcagttcaa cggcacgacg tcatgggacc gcacgaacta cttcgagacg ttcgacgcga
      541 gcgacgcgaa tctcgagtgg gcgctcgatc tcgagtcgga tcgcatgatc aacgcgttcg
      601 tcgcgcaaga agacctcgac accgagatga cggtcgtgcg caacgagttc gagcaaggcg
      661 agaacagccc gaccggcgtg ctgttcgagc gcgtgatgtc cgcggcatat ctgtggcacg
      721 gctacggcaa ctcgccgatc ggcagccgca gcgacatcga gaacgtgccg atcccgcgct
      781 tgcgcgcgtt ctacgagcgc cactatcaac ccgacaacgc cgtgctcgtc atcgccgggc
      841 gcttcgagga gcagcgggcg ctcgagctga tcgccaagta tttcagctcg attccgcggc
      901 cgcagcgcga gctcaccgac aactacacgg tcgagccgac gcaagacggc gagcgcgagg
      961 tcgtcgtgcg ccgcgtcggc gacgtgcaga tcctgatggc cggctatcac gtgccggcag
     1021 ggtcgcacga ggacttcgtg ccgctgcaga tcgcggccga ggtgctgggc gacgtgcctt
     1081 ccggccgcct gtataaagcg ctcgtcgagc cgggactggc ctcgcaaatc gccgtgcagg
     1141 aaatgcagct gcgcgacccc ggcatgatcg tcttctttgc gctcgtgccg aaggagaagt
     1201 ctctcgaagc ggcgcgcgag gcgatgatcg cgacgatcga cgcgctcgcc gcgaatccag
     1261 tcaccaccga agaggtcgag cggattcgca accaggcgat gtcgagcttc gagcagcaga
     1321 tgaacaattc gcagggcatc gcgatccagc tcagcagctg ggcggcgatc ggcgactggc
     1381 gcatgctgtt cctcgatcgg gaccgcgtgc gcaccgcgtc ggcggaaagc gtgcagagcg
     1441 cagcgctgaa gtatttcaag ccgtcgaacc gcaccgtcgg gttgttcgtg cccggcgacc
     1501 ccgatcgcag cgagatcccg gcgcgtcccg acgtcacggc gatgctgcag ggttacaaag
     1561 gcgaccaggc gcgcgccgac ggcgaggcgt tcgatccgac gccggccaac atcgatgcgc
     1621 gcacgacgcg cgtcgatcta ccgggcggcg tcaagctcgt gatgttgccg aaggagaccc
     1681 gcggcgacgc cgtgaacgcg ctgattcgcc tcaactacgg cgacgagaag agcctgacgg
     1741 gaccggggcg gcttgcgggc gtcacgacgc aaatgctgat gcgcggcacg cgcgcgaagt
     1801 cgcggcaaga catccaagac gagctcgcgc ggctgcagag ccagctcaac gtcggtggcg
     1861 gcgccggcct cgcgtccgcg aacatacaga gcactcgcgc gaatctgatc gaggtgctga
     1921 cgctcgcgat cgaggtgctg cgcgagcctg cattccccga cgccgagctc gcaacgctga
     1981 agcaacagac gattaccggc ctcgaagcgt cgcgcagcga gccgcaagcg atcgtgtcgc
     2041 gcgcatacgc gaggcactgg gcgcgcaact acaagcccga cgatccgcgc tacgtcacga
     2101 cgatcgacga ggacctcgct ttcatcaacg gcgtcaggac ggcgcagctc cgcgactttc
     2161 acaaggactt cttcggcgcg tcgaacgccg aggtcgtcgt catcggcgac ttcgatccgg
     2221 agcaggtccg cgcgttgatc gccagccggc tcgacggctg gaagagcccg aagccgttca
     2281 gcgacgtgtt gaaccgctac gcggacctcg cgcacgatgc gatcagccaa gacttcaaca
     2341 cgcccgacaa agagaacgcg atgttcttgg ccggcatgcg gatcgagatg cgcgactcgc
     2401 atccggacta tccggccatg ctattcggca actacatctt gggctcgggg cccgcttcgc
     2461 gcctgttcgg ccgcatccgc ggcaaggaag gcctcagcta cggtgtcggc tcgagcttcg
     2521 gcgcctcgcc gcgcagcgac ggcgcgtcgt tcacggtcaa cgcgatcgcg gcgccgcaga
     2581 acgccgccaa ggtcgaggcg tcgttccgcg acgagctcgc gacggtgctg cgcgagggct
     2641 acacgcagga agagttcgac gccgcgaaag tcagctgggc gcaagggcgt caggtcagcc
     2701 gagcacaaga cgccggcctc gtcggcacgc tcggcttgtg gacgcacgtg ggccgcacga
     2761 tggccttcga cgccgacctc gaggcgaagg tcgccgcgtt gacggtcgag caagtccgcg
     2821 acgcgatgaa ccggcatctc gacgtcaaca agatgacgtt catgaagggc ggcgacttcg
     2881 agtccgtcag cgcgggcgcc ggcggagcgc cgtgagctga cgactcgcgc tgcggaagtc
     2941 gtttccggcc ctccggcgcg acgaccgtcg ccggcggtgg tttgccgccg cggagcgcgc
     3001 ccgttatcgt cgcggccatg aatcggccac gttcctcgcg tcagcactac gacgttttcc
     3061 gccgcgacta ccgcgaaggg acgctcgacg acaagcttgc gaccgtcgac aagcaaccgg
     3121 tcaaagcgcc ggccgtcaaa gcgcgccgcg agtacctgaa ggactacctg cgctggctct
     3181 ggccggagcg ctggaccgtg gccgccgtct tcgcgctggc cgtgacaacg gcgggactcg
     3241 acatgatctc gccgctgttc atgcgcttca tcacggacca cgtgctgctc gacacggccg
     3301 ccgcaacggc cgaccgcttc cgcctcctga atctcgccgg cggcctattc ctgatgctga
     3361 ttacggtgtc ggccggcctg aacatcgcgc gcgattaccg gcagcgcctc gtcaacacgc
     3421 gcgtcatgcg gtccctgcgc cgatcgctgt tcgagcgttt gctgcggctg ccgctgtcga
     3481 cgctctggga catgaagacc ggcggaatcc tgtcgcggct ttccggcgac gtcgactcga
     3541 cgacgggcct gttgcaaatg gcgatcgtgt cgccgtcgat ttcggtcatt cgcttgttga
     3601 tcgcgatgac cgtgctgttc gcgctgaact ggcagctcgc gctc
//
LOCUS       DASSNN010000362         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_569093, whole genome shotgun sequence.
ACCESSION   DASSNN010000362 DASSNN010000000
VERSION     DASSNN010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569093"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..>2300)
                     /locus_tag="VFL84_06645"
     CDS             complement(118..>2300)
                     /locus_tag="VFL84_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608336.1"
                     /translation="GRDGIENAPIRDDAKRLLGMHVRKLNLDESTVYFFDAELAQQWL
                     DSHERIPDSMKPKDDEPEDDSECGKVSINGIVDCAEDAGQAIEDEYERARERAEDWYD
                     DSTEDLADAWNEAKNCFSDHVLPGGTVPVKFDIEPAMAVNLSQSGSRGGASGNVTGSI
                     GLGIPMDGDLQAKVDFFYIPCLPFVVRPRSMTVTGDLTVGEVLGIQATATGEFNQRYT
                     IPPTGGPRIPIQVIPIVIGGVPVAIIDVSAYIEGEIEVTANGNVVGTATLSNEHKTTF
                     DFRCDGHGCETGSRGHPTASTPITTTQAVQIEGKVTAKPGIYTALMFTLNVNVLGARL
                     GPQPFLEGTAVGCGAATATQTNGTQTAGDQNAVLAADLDWGVDFRAEALAGGKVVGNS
                     YRREIIPHKDNHIWFRDLIPGGSSALHAAIAGPTEAATGQPTAYKLSLPSCYPYDEDA
                     KYRVTWTGNATPSSTPASACSWQAGRGDCRADPARELDVRFAWPAEGSYTLTAALIGD
                     AHRTFDPAPAAAEMTVAVSGAPSAAGTASGGSSTGTTETGGPRSAPSGPSTSDSGSRP
                     GGGGAAPTAGRPGGAADSSCCSIVPNATMRGRLGRLVVAFPQEVAARIEVYRPGEERA
                     IQSGYGNQAYDLLPGTYEVSISGKRVAGVTVRSGNDTQIKVGVLRVNASDGTRIDLAD
                     PISKERVTGGYGTQVFGLPIGQIGVQVAGQTEAVVIEAGKVAEF"
BASE COUNT          320 a          791 c          767 g          422 t
ORIGIN      
        1 gacgatccgc gctatccggg cgagctcgtc aatctcgcgc tgcggctcgg cgtcaacgtc
       61 gcgatgtacg cgatgacgca ctgagcgcgc ggcggcgccg ccgaaggcgc ggggccgtca
      121 gaactccgcg accttgcccg cctcgatcac gacggcctcg gtctggccgg cgacctgcac
      181 gccgatctga ccaattggga ggccgaatac ttgcgtcccg tagcctcccg tcacgcgctc
      241 cttgctgatg ggatcggcga ggtcgatgcg tgtaccgtcg ctcgcgttga cgcgcagcac
      301 gccgaccttg atctgcgtgt cgttgcccga gcgcaccgtg acgcccgcga cgcgcttgcc
      361 cgagatgctg acctcgtacg ttccgggcag caggtcgtac gcttgattgc cgtaacccga
      421 ctgaatcgcg cgctcttcgc cggggcgata cacctcgata cgggccgcga cttcctgcgg
      481 gaacgcaacg acgaggcgcc cgagccgacc gcgcatcgtg gcattcggca cgatgctgca
      541 gcacgatgag tcggcggccc cgcccggccg gcccgcggtt ggcgcggcgc cgccgccgcc
      601 cggtctgctg ccgctgtcgg aggtgctggg gcccgatggc gcgctgcgag gtccgccggt
      661 ctccgtggtt cccgtcgagc tgccgccgct cgccgtgccc gcggcgctcg gcgcccccga
      721 gaccgcaacg gtcatctcgg ctgccgccgg tgcaggatcg aacgtccgat gagcgtcacc
      781 gatcagcgcg gccgtcagcg tgtagctgcc ttcggcgggc cacgcgaagc gcacgtcgag
      841 ctcgcgtgcc ggatcggcgc ggcagtcgcc gcgccccgct tgccagctgc acgccgaagc
      901 cggagtcgag gagggcgtcg cattacccgt ccatgtgacg cggtatttcg cgtcttcgtc
      961 gtacgggtaa cagcttggca agctcagctt gtacgcggtg ggttggcccg tcgccgcctc
     1021 ggtagggccg gcgatcgccg cgtgcagcgc gctcgacccg ccggggatca gatcccgaaa
     1081 ccagatgtga ttgtctttgt gggggatgat ctcccggcgg taactgttgc cgacgacttt
     1141 gccgccggcc aacgcttcgg cgcgaaagtc gacgccccaa tcgaggtcgg ccgcgagcac
     1201 ggcgttctgg tcgccggccg tctgggtgcc gttcgtctgc gtggccgtgg cggccccgca
     1261 tcccacggcg gtgccctcga ggaacggttg cggccccaag cgcgcgccga gaacgttgac
     1321 gttcagcgtg aacatcagtg ccgtgtagat gccgggtttc gccgttacct tgccttcgat
     1381 ctgcacggcc tgcgtggtcg tgatgggagt gctcgcggtc ggatggccac gcgagccggt
     1441 ctcgcagccg tggccgtcgc acctgaagtc gaacgtcgtc ttgtgctcgt tgctgagcgt
     1501 cgctgtgccg accacgttgc cgttcgccgt gacctcgatc tcgccttcga tgtaggcgct
     1561 gacgtcgatg atggcaacgg gcacgccgcc gatgacgatc ggaatcacct ggatcgggat
     1621 tcgcggcccg ccggtcggcg gaatcgtgta cctctggttg aactcgccgg tggccgtcgc
     1681 ctggatgccg agcacctcgc cgacggtcaa atcgccggtc accgtcatgc tgcgcggccg
     1741 cacgacgaac ggcaggcacg ggatatagaa gaagtccacc ttcgcctgga ggtctccgtc
     1801 catcggaatg cccagaccga tgctgccggt gacgttgccc gacgctccgc cgcgtgagcc
     1861 gctttgcgaa aggttcacgg ccatcgccgg ctcgatatcg aacttcacgg ggaccgtgcc
     1921 gccgggcaag acatggtccg agaaacagtt cttcgcctcg ttccacgcat cggcgaggtc
     1981 ttccgtggag tcgtcatacc aatcctcggc gcgctcgcgc gcgcgctcgt actcgtcttc
     2041 gatcgcttgg cccgcgtcct ccgcgcaatc gacgatgccg ttgatgctga ccttgccgca
     2101 ctccgagtcg tcctcgggct cgtcgtcttt cggcttcatc gaatcgggaa tgcgctcgtg
     2161 cgaatcgagc cattgctgag cgagctccgc atcgaagaag tagacggtcg attcgtcgag
     2221 gttcagcttc cgcacgtgca tgcccagaag acgcttggcg tcgtctcgta tcggggcgtt
     2281 ctcgatcccg tcacgcccgt
//
LOCUS       DASSNN010000363         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1196602, whole genome shotgun sequence.
ACCESSION   DASSNN010000363 DASSNN010000000
VERSION     DASSNN010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196602"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VFL84_06650"
     CDS             complement(<1..1073)
                     /locus_tag="VFL84_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HET7608337.1"
                     /translation="MTTNGRWSSGMLRTEDWWAVWIGLGLFALSLASLGGVDLVGWMA
                     RPRAWEWTDVAGAFAWSKLFAPAGAAYAGWHPLVSFATTYAVFTALFALGARSLRLDV
                     RRFVLGFTILFLVTWLAWIAGNELHLTMVDATVDGRNRYRDAGLSWGLQLGEGAPYLL
                     ALLAGLALGNFGRTFAARYLAEAARPEWYIKTAIVFLGVNLGSQTMEASGFAAELILT
                     GAAATFVAYLFFWPIVYALARRVFGFKRDVSAVLASGISVCGASAAIATAGAIRAKPI
                     IPVTVSMVVVIFAMIELIVLPGLYTAFMPDQPIVNGAALGMTVKTDGADAAAGALLDE
                     LMIARNLQLTGMRWQDGWILSASL"
     gene            complement(1070..1318)
                     /locus_tag="VFL84_06655"
     CDS             complement(1070..1318)
                     /locus_tag="VFL84_06655"
                     /inference="COORDINATES: protein motif:HMM:NF036574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5395 family protein"
                     /protein_id="HET7608338.1"
                     /translation="MTLVLSYDRDGWHAAGEGVDVAHSELRGLETLLEERLAGAAPVD
                     VHLAFDMASLPRWLHQYHGHYCNYTLRVGRGPRRSGRA"
     gene            complement(1324..1491)
                     /locus_tag="VFL84_06660"
     CDS             complement(1324..1491)
                     /locus_tag="VFL84_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608339.1"
                     /translation="MTHDVTPPTAPSDDALSGAPEPWERWETSLVLGSLLLGCVGLVL
                     LGWLVARFILP"
     gene            complement(1545..>2305)
                     /locus_tag="VFL84_06665"
     CDS             complement(1545..>2305)
                     /locus_tag="VFL84_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7608340.1"
                     /translation="NGDLEHDLAALLETVGAAAQRAGTALVLFIDELQYVKEDELAAL
                     ITGLHRTAQRKLPVGLVGAGLPQLRGRMGRAKSYAERLFDFPEIGALPPPAARQALVK
                     PALDQGVEIAEDALEQILVETHRYPYFLQEWGKHAWQVAERSPITLKDVRNASAVAIA
                     ALDASFFRVRFDRLTLAEKRYVRAMAELGPGPHRSGDVAEAVGRKVTSLAPIRNQLIA
                     KGMIYSPSHGDTAFTVPLFDEFMRRVMPGDDWRE"
BASE COUNT          400 a          811 c          773 g          321 t
ORIGIN      
        1 agcgacgcgc tcaggatcca gccgtcctgc caccgcatgc cggtcagctg caagttgcgc
       61 gcgatcatga gctcgtcgag cagcgcgccg gccgccgcat cggccccgtc ggtcttgacc
      121 gtcatgccga gtgcggcgcc gttcacgatc ggctggtccg gcatgaaagc cgtatagagg
      181 cccggcaaga cgatcagttc gatcattgcg aagatcacga cgaccatcga gaccgtgacg
      241 gggatgatcg gcttcgcgcg gatcgcgccg gccgtcgcga tggccgccga cgcgccgcag
      301 accgagatgc ccgacgcgag cacggccgac acgtcgcgct tgaaaccaaa cacgcggcgc
      361 gcgagcgcgt agacgatcgg ccagaaaaac aagtacgcga cgaacgtcgc cgcggcgccg
      421 gtcaggatca gctcggcggc gaagccgctc gcttccatcg tctgcgagcc taaattgacg
      481 ccgaggaaaa cgatggccgt cttgatgtac cactcgggac gggcggcttc ggccagatac
      541 ctcgccgcaa acgttctgcc gaaattgccg agcgccaagc ccgcgagcaa cgcgagcaga
      601 tacggcgcgc cttcgccaag ctgcaagccc cagctcaagc ccgcgtcgcg gtagcgattg
      661 cggccgtcga cggtcgcatc gaccatcgtc aggtgcagct cgttgccggc gatccacgcg
      721 agccaggtca cgaggaacaa gatcgtgaaa ccgagcacga atcggcgcac gtcgagccgc
      781 agcgagcgcg cgccgagcgc gaacagcgcc gtgaacacgg cgtacgtcgt cgcgaacgag
      841 acgagcggat gccagcccgc gtaagcggcg ccggccggcg cgaacagctt gctccacgcg
      901 aacgcgccgg cgacgtcggt ccattcccag gcccttggcc gcgccatcca gccgacgagg
      961 tctacgcctc cgagcgacgc gaggctcagc gcgaagaggc cgagaccgat ccagacggcc
     1021 caccagtcct cggtgcgcag catgccgctc gaccagcgcc cgttcgtcgt cacgcgcgcc
     1081 cactcctgcg cggcccgcgg cccacgcgca gcgtgtagtt gcagtagtga ccgtggtact
     1141 ggtgcagcca acgcggcagc gacgccatgt cgaatgcgag gtgcacgtcg accggcgccg
     1201 cgccggccag ccgttcctcg agcagcgtct cgaggccgcg cagctcggaa tgcgcgacgt
     1261 cgacgccctc gccggccgca tgccagccgt cgcgatcgta cgaaagcacg agcgtcatgg
     1321 aattcacggc aggatgaacc gcgcgacgag ccagccgagc agcacgagcc cgacgcagcc
     1381 gagcaaaagg ctgccgagca cgagcgaagt ttcccaccgc tcccatggtt cgggcgcgcc
     1441 ggacaaagcg tcgtcgctcg gcgccgtcgg cggcgtcacg tcgtgcgtca tgcggattcc
     1501 cccgatcgca atctgcgctg cgttcgcgag agtataagcg cgttctattc gcgccagtca
     1561 tcccccggca tgacgcggcg catgaactcg tcgaacagcg gcaccgtgaa cgccgtgtcg
     1621 ccgtggctcg ggctgtagat catgcccttc gcgatgagct gattgcggat cggtgcgagc
     1681 gacgtcactt tgcgcccgac ggcttcggcc acgtcgccgg agcggtgcgg cccggggccg
     1741 agctcggcca tcgctcgcac gtagcgcttc tcggcgagcg tgagccgatc gaagcggacc
     1801 ctgaagaagc tcgcgtcgag cgcggcgatc gccacggccg acgcgttccg cacgtccttc
     1861 agcgtgatcg gcgaccgttc ggcgacttgc cacgcgtgct tgccccattc ctgcaggaag
     1921 tacggatagc gatgcgtctc gacgagaatc tgctcgagcg cgtcctcggc gatctcgacg
     1981 ccttggtcca gcgcgggctt gacgagcgct tggcgcgccg cgggcggcgg caacgcgccg
     2041 atctcgggga aatcgaacag ccgctcggcg tacgacttgg cgcgccccat gcggccgcgc
     2101 agctgcggca ggccggcgcc gacgagcccg accggcagct tgcgctgcgc cgtgcggtgc
     2161 aagcccgtga tcaaggccgc gagctcgtct tctttgacgt actgcagctc gtcgatgaag
     2221 agcacgagcg cagtgccggc ccgctgcgcc gccgcaccga ccgtctcgag cagcgcggcg
     2281 aggtcgtgct cgagatcgcc gttgt
//
LOCUS       DASSNN010000364         2761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_299870, whole genome shotgun sequence.
ACCESSION   DASSNN010000364 DASSNN010000000
VERSION     DASSNN010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299870"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFL84_06670"
     CDS             complement(<1..469)
                     /locus_tag="VFL84_06670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET7608341.1"
                     /translation="MTTIVGISGSLRAKSFNSSLLRAAAQAMPRDATLEIATIKGIPL
                     YDGDVEETQGVPDVVAKLKDRIVAADGLLLVTPEYNNSLPGVFKNAIDWLSRPPADIP
                     RVFGKRPVGVIGASPGGFGTLLSQTAWLPVLKTLGTAAYFGGRLQLSRADTVFD"
     gene            complement(488..1879)
                     /gene="zwf"
                     /locus_tag="VFL84_06675"
     CDS             complement(488..1879)
                     /gene="zwf"
                     /locus_tag="VFL84_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET7608342.1"
                     /translation="MSKEKTDALVIFGITGDLAHKKIIPALQSLVKHGALDVPVIGVA
                     RGGSLEKLRERIHDSLAHSEEGIDDGAYAKLLELVRYVEGDYREPKTFAALRKALGAA
                     KHPLHYLALPPSLFATVADHLRESGCADGASVVVEKPFGRDRASALELNEALHRTFDE
                     PSVYRIDHYLGKESVQNLLFFRFANSFLEPIWNRNYVDNVQVTMAESFGVGTRGAFYE
                     EVGAIRDVVQNHMLQIVAHLAMEPPVAADADSLRDEKVKVFKAIRSASPDALVRGQYR
                     GYRKEQGVAPDSNVETYAALRLDICSWRWAGVPFYLRTGKLLDTTATEVVATLKPPPQ
                     DVFPGNDAPNYVRFRLGPKRVAIGLGAVAKRPGPEMRGREIELLVCDTGNEEVGAYER
                     LIGAALVGDHSLFAREDGVLEAWRIVDPLLRAPRAPEIYEPGSRGPAAADAVVEGSGE
                     GWRPIREDIGDCS"
     gene            complement(1886..>2761)
                     /gene="gnd"
                     /locus_tag="VFL84_06680"
     CDS             complement(1886..>2761)
                     /gene="gnd"
                     /locus_tag="VFL84_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008533064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET7608343.1"
                     /translation="AAASLEELVAKLTPPRAIWIMIPAAAVEGVIETLVPRLAAGDVL
                     IDGGNSHFVDDVRRAKTLGERGLHYLDVGVSGGIFGLELGFCLMIGGDAATAARLTPL
                     FATLAPGDVKKPPSKSTAAHGYLHCGPSGAGHFVKMVHNGIEYGLMAAYAEGFNLLRQ
                     ANAGTRAARVDAETAPHTVQSAHLEYDLDVAAIAELWRHGSVVRSWLLDLTSAALARN
                     PTLDGFVGEVADSGEGRWTINTAVDAGVPVPVLASALFARFSSRNHDDFANRLLSAMR
                     QEFGGHVERPGSPKR"
BASE COUNT          424 a          921 c          971 g          445 t
ORIGIN      
        1 cgtcgaagac cgtgtcggcg cgcgagagtt gcaagcggcc gccgaagtag gcggcggtgc
       61 cgagcgtctt cagcacggga agccacgcgg tttgcgacag caacgttccg aatcctcccg
      121 gcgacgcgcc gatcacgccg accgggcgct taccgaacac gcgcgggata tcggccggcg
      181 ggcgcgacag ccagtcgatt gcattcttga acacgccggg aagcgagttg ttgtactcgg
      241 gcgtgacgag cagcaagccg tcggccgcga cgatgcgatc cttgagcttc gcgacgacgt
      301 ccggcacgcc ctgcgtctcc tcgacgtcgc cgtcgtagag cggaatccct ttgatcgtcg
      361 cgatctcgag cgtcgcatcc cgcggcatcg cttgcgccgc ggcgcgcagc agcgagctgt
      421 tgaacgattt cgcgcgcaag cttccggaaa ttccgacgat tgtggtcatg gtagtcaccc
      481 cttcctgcta agagcagtcg ccgatgtcct cgcgaatcgg gcgccagccc tcgcccgacc
      541 cttcgacgac cgcgtcggcg gccgcgggac cgcgcgagcc gggctcgtag atctcgggcg
      601 ctctcggcgc tcgcagcagc ggatcgacga tgcgccacgc ctcgagcacg ccgtcctcgc
      661 gcgcgaacaa cgagtgatcg ccgacgagcg cggcgccgat gagccgctcg taggcgccga
      721 cctcctcgtt gcccgtgtcg cagacgagca gctcgatctc gcggccgcgc atctcggggc
      781 cgggccgttt cgcgaccgcg cccaggccga tcgcgacgcg cttcggaccg agccggaagc
      841 gcacgtagtt cggcgcgtcg ttgcccggga acacgtcttg cggcggcggc ttcagcgtcg
      901 ccacgacttc ggtcgcggtc gtgtcgagga gcttgccggt gcgcagatag aaaggcacgc
      961 cggcccagcg ccacgagcag atgtcgaggc gcagcgccgc atacgtctcg acgttcgagt
     1021 cgggtgctac gccttgctcc ttgcgatagc cgcgatactg gccgcgcacg agcgcgtcgg
     1081 gcgaggcgct gcggatcgcc ttgaacacct tgaccttctc gtcgcgcagc gaatcggcgt
     1141 cggccgcgac gggcggctcc atcgcgagat gcgcgacgat ctgcagcatg tgattttgga
     1201 cgacgtcgcg gatagcgccg acttcttcgt agaacgcgcc gcgcgtgccg acgccgaagc
     1261 tctccgccat cgtgacctgc acgttgtcga cgtagttgcg gttccagatc ggctcgagga
     1321 acgagttcgc gaagcggaag aacaagaggt tctgcacgga ctccttgccg aggtagtgat
     1381 cgatgcggta gacggacggc tcgtcgaagg tgcggtgcag cgcttcgttg agctcgagcg
     1441 ccgacgcgcg gtcgcgcccg aacggcttct cgacgacgac gctggcgccg tcggcgcagc
     1501 cggactcgcg caggtgatcg gccaccgtcg cgaacaagct cggcggcaac gcgaggtagt
     1561 gcagcggatg cttcgcggcg ccgagcgcct tgcgcaacgc ggcgaacgtc ttcggctcgc
     1621 ggtaatcgcc ttctacgtag cgcacgagct cgaggagctt cgcgtaggcg ccgtcgtcga
     1681 tgccttcctc gctgtgggcc aagctgtcgt gaatgcgctc gcgcaatttc tcgagcgagc
     1741 cgccgcgcgc gacgccgatg acgggcacgt cgagcgctcc gtgcttcacg aggctttgca
     1801 gcgcgggaat gatcttcttg tgcgcgaggt cgcccgtgat accgaatatg acgagcgcat
     1861 ccgttttttc cttggacacg ggctgctacc gcttcgggct gcccgggcgc tcgacgtggc
     1921 cgccgaattc ctggcgcatc gccgagagca atcggttcgc gaaatcgtcg tggttgcgcg
     1981 agctgaagcg cgcgaacagc gcactcgcga gcacgggcac gggcacgccg gcgtcgacgg
     2041 cggtgttgat cgtccagcgg ccctcgcccg agtccgcgac ttcgccgacg aagccgtcga
     2101 gggtcggatt gcgcgcgagc gcggcgctcg tcagatcgag cagccacgag cgcacgacac
     2161 ttccatggcg ccagagctcc gcgatcgcgg cgacgtcgag atcgtactcg aggtgcgcgg
     2221 attgcacggt gtgcggcgcg gtctcggcat cgacgcgcgc ggcgcgcgtg ccggcgttcg
     2281 cttggcgcag cagattgaaa ccttcggcgt aggcggccat gaggccgtac tcgatgccgt
     2341 tgtgcaccat cttgacgaag tggccggcgc cgctcgggcc gcagtgcaag tacccgtgcg
     2401 cggcggtgga cttgctgggc ggctttttga cgtcaccggg cgcgagcgtc gcgaacagcg
     2461 gcgtgagccg cgccgcggtc gcggcgtctc cgccgatcat gaggcaaaac ccaagctcga
     2521 ggccgaagat gccgccgctg acgccgacgt cgagatagtg caggccgcgc tcgccgaggg
     2581 tcttcgcgcg gcgcacgtcg tcgacgaagt gcgagttgcc gccgtcgatc aggacgtcgc
     2641 cggccgcgag ccgcgggacg agcgtctcga tgacgccctc gacggcggcg gccgggatca
     2701 tgatccagat cgcgcgcgga ggcgtgagct tcgcgacgag ctcctcgagc gatgccgcgg
     2761 c
//
LOCUS       DASSNN010000365         2064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_443090, whole genome shotgun sequence.
ACCESSION   DASSNN010000365 DASSNN010000000
VERSION     DASSNN010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443090"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /gene="cysD"
                     /locus_tag="VFL84_06685"
     CDS             complement(<1..861)
                     /gene="cysD"
                     /locus_tag="VFL84_06685"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019960983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HET7608344.1"
                     /translation="MNTGPLAPLRAARPGRARKPTHLELLEAESIHIMREVVAEFERP
                     VMLYSIGKDSSVLVRLAQKAFAPAPIPVPLLHIDTTWKFRAMIELRDRTARELGLDLR
                     VHTNRRALAEGASPFTWGSNKYTQVMKTHALLDALREGRFDAAIGGARRDEERSRAKE
                     RVLSFRDAHGQWDPKNQRPELWDLYNGKVKKGESVRAFPLSNWTELDVWQYILRESIE
                     IVPLYFAAERPTVTRDGLILMVDDDRFPLNGEEPVQRKVRFRTLGCYPLTGAVESEAA
                     TVEEVVAEMLL"
     gene            complement(919..>2064)
                     /locus_tag="VFL84_06690"
     CDS             complement(919..>2064)
                     /locus_tag="VFL84_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608345.1"
                     /translation="TTGGSIKRLHDEDGDGYFQPTEIKEYANTGGNGNNCHIDVANGY
                     LYAGTATGVKRWPWSPTATDGGAGEDVVTGQPNGGHTNHTVHVYDGYLYVHSGSAGNA
                     TRSNTTGDKSEYDDNRSMIRRFNLASFTPGTPFQWLTGEIVTQGLRNANGFTRNAQGR
                     MYAVVNGLDEIRYQGNDVHNDNPGEQVLEIAMGKKYGYPFCFTAQRVVTGGSTVVPPG
                     TQLVNVNLDDGTNPHDDAWCAANSDKPATFIQAHSAPLDIVFFDGADPTGNLPEKYRG
                     GAFVALHGSWDRSPATGYKVIWIPFNADGTAPMPTSTATDTTFPYETVFGRGTSAGPE
                     DGSWAWSDDDGYSDNPRPAGVAIGPVDGALYFTTDGKGYLYRLGIKH"
BASE COUNT          273 a          694 c          730 g          367 t
ORIGIN      
        1 gaggagcatc tcggctacga cttcctcaac ggtcgcggcc tcgctctcga ccgctccggt
       61 cagcgggtag caaccgagcg tccggaaacg caccttgcgc tgcaccggct cctcgccgtt
      121 cagcggaaag cggtcgtcgt cgaccatgag gatgagcccg tcgcgtgtca ccgtcgggcg
      181 ctcggcagcg aaatagagcg gtacgatctc gatgctctcg cgaaggatgt attgccagac
      241 gtcgagctcg gtccagttcg acaaggggaa cgcgcgcacg ctctcgccct tcttcacctt
      301 gccgttgtag aggtcccaaa gctcggggcg ctggttcttc ggatcccact ggccgtgcgc
      361 gtcgcggaac gagagcacgc gctccttcgc gcgcgagcgc tcctcgtcgc gccgcgcgcc
      421 gccgatcgcc gcgtcgaagc gcccttcgcg cagagcgtcg agaagcgcgt gcgtcttcat
      481 gacctgggtg tacttgttcg agccccacgt gaacgggctc gcgccctcgg cgagcgcgcg
      541 gcggttcgtg tggacgcgca gatccagccc gagctcgcgc gcggtcctgt cgcggagctc
      601 gatcatggcg cggaacttcc acgtcgtgtc gatgtgcagg agcgggacgg ggatgggcgc
      661 cggcgcgaac gccttctgcg cgaggcgcac gaggaccgac gagtccttgc cgatcgagta
      721 gagcatcacg ggccgctcga actcggcgac gacctcccgc atgatgtgga tgctctccgc
      781 ctcgaggagc tcgaggtgcg tcggcttgcg cgcgcggccg ggacgcgcgg cgcggagggg
      841 tgcgaggggg cccgtattca cgatcgtgaa tacgaatcta tcttactaga cgtgcccgcg
      901 caacccgcgc ggcccgcgtc agtgcttgat gccgagccgg tacaggtacc ccttgccgtc
      961 cgtcgtgaag tagagcgcgc cgtcgaccgg cccgatcgcg acgcccgcgg gccgcgggtt
     1021 gtcgctgtag ccgtcgtcgt ccgaccacgc ccacgagccg tcctccggcc ccgccgacgt
     1081 gccgcggccg aagaccgtct cgtacgggaa cgtcgtgtcc gtcgcggtcg acgtcggcat
     1141 cggcgcggtg ccgtccgcgt tgaacgggat ccagatgacc ttgtagcccg tcgccgggga
     1201 gcgatcccac gagccgtgga gcgcgacgaa cgcgccgccg cggtacttct cgggcaggtt
     1261 gcccgtcggg tccgcgccgt cgaagaagac gatgtcgagc ggcgccgagt gcgcctggat
     1321 gaacgtcgcc ggcttgtccg agttcgccgc gcaccaggcg tcgtcgtgcg ggttcgtgcc
     1381 gtcgtcgagg ttcacgttga cgagctgcgt cccgggcggc acgaccgtcg agccgcccgt
     1441 cacgacccgc tgcgcggtga agcagaacgg gtagccgtac ttcttcccca tggcgatctc
     1501 gaggacctgc tcgccggggt tgtcgttgtg gacgtcgttg ccctggtagc ggatctcgtc
     1561 gaggccgttg acgacggcgt acatgcgccc ctgggcgttg cgcgtgaagc cgttcgcgtt
     1621 gcggaggccc tgcgtcacga tctcgcccgt gagccactgg aagggcgtgc ccggcgtgaa
     1681 cgacgccagg ttgaagcggc ggatcatcga gcggttgtcg tcgtactcgc tcttgtcccc
     1741 ggtcgtgttg ctgcgcgtcg cgttgccggc ggagccggag tgcacgtaga ggtagccgtc
     1801 gtagacgtgg accgtgtggt tcgtgtggcc gccgttgggc tggccggtca cgacgtcctc
     1861 gccggcgccg ccgtccgtcg ccgtcggaga ccacggccag cgcttcacgc cggtcgcggt
     1921 ccccgcgtag aggtatccgt tcgcgacgtc gatgtggcag ttgttcccgt tgccgccggt
     1981 gttcgcgtac tccttgatct cggtcggctg gaagtagccg tcgccgtcct cgtcgtgcag
     2041 gcgcttgatc gagccgcccg tcgt
//
LOCUS       DASSNN010000366         6438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1197381, whole genome shotgun sequence.
ACCESSION   DASSNN010000366 DASSNN010000000
VERSION     DASSNN010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197381"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VFL84_06695"
     CDS             complement(<1..429)
                     /locus_tag="VFL84_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7608346.1"
                     /translation="MRQFDLLVLGGGSGGLAAAQRAAEHGARVAIFEPARLGGTCVNV
                     GCVPKKVMWNAAELQAALAHAPYYGFDVKTGGHDWAKLKLERDAYVLRLNGIYERNLD
                     KKGIVTIRDAAAFRSPTELVTTTGERFSAPHIVIATGGAPI"
     gene            529..2565
                     /locus_tag="VFL84_06700"
     CDS             529..2565
                     /locus_tag="VFL84_06700"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET7608347.1"
                     /translation="MTETTEPGPNPLLAARVLPAYSAVKPEHVEPAVRELLAAQRRAL
                     VAAESVATPDLDWLRNLERISVELDRVWGPVWHLNSVVSTPPLRDAFNRCLPLVTEFG
                     TELAQNETLYRHFSALKASVGTAKPVERQLIDNALRDFKLGGVTLTGAPKQRFREVMQ
                     ELAAKQAKFEQNVMDATDAFEHRETDPAALAGLPQFVQDRAAALAKERDFEGFSLRLD
                     PPTYQTVMAHAESAALREKYYRAWNTRASDQGQNPQWDNGPLIDEILALRHEAAQLVG
                     FKTFAEFSLATKMAESPQRVIEFLRDLARRSRADARDELAMLTEYAGRKLAAWDVPFY
                     AERLRQEKLGLAEEELRPYFALPRVLDGLFELCGRLFDIEVKQAPPPDTWHDSVRYYE
                     LKRRDGTLIGSFFTDLYARPNKRGGAWMGGAVSRAKLNGHAQTPVAHLVCNFNAPAGT
                     TPSLLTHSEVVTLFHEFGHALHHLLTEVDYPSLAGTNGVAWDAVELPSQFLENFAWRP
                     EMLTEIGRHYETGAPLPNDKIETLKRSRTFLSGLAMVRQLEFALFDFRLHEEYSAERG
                     SRVRDVLDEVRREVAVVEPPPFNRFAHSFGHVFGGGYAAGYYSYKWAEVLAADAFAAF
                     EEAGVFDRATAERFRRAVLATGGSRKALDAFVDFRGRQPKLDALLKQSGIAERQ"
     gene            2569..3342
                     /gene="xth"
                     /locus_tag="VFL84_06705"
     CDS             2569..3342
                     /gene="xth"
                     /locus_tag="VFL84_06705"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET7608348.1"
                     /translation="MKVATWNINSLRVRLPHVLKWLEREQPDVLALQETKIQNDEFPA
                     DAFAQYGYVAAFSGQSAYNGVAILSRAPLDDITYDIGGGYTDEQRRVLGVTVRGARLW
                     SLYVPNGQSVDSEKYRYKLEWLAALRARLEDEIARHPKLLVVGDFNVAPDDRDVHNPE
                     AWRGKIMCSPAERAALTGLCGLGLRDAFRLFSQPEKVFSWWDYRAGAFRRNQGLRIDL
                     VLASDALSQTCSGCRIDKEPRGWDRPSDHVPVIAEFALP"
     gene            3415..4464
                     /locus_tag="VFL84_06710"
     CDS             3415..4464
                     /locus_tag="VFL84_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7608349.1"
                     /translation="MPIEDELQDRRNDYDVTNTVQVSSSRHVRAAVADVFAALYPSSS
                     FDSVWMAFHDFERVFRGLDPNYHAVDTTYHDMQHTLDMTLALARLIAGHEMSVEPADR
                     LGAERAELAIVSALFHDVGYLRHKDRDHAAVNGAVFTRVHVSRSGHYLEKYLPRIGLE
                     QFVPVVARIVHFTGYELKLDQIELEQPKDSVVGHLLGTADLVAQLADRCYLEKCRDRL
                     YPEFVLGGVAIDERPDGAVLYRSAEDLLGKTLSFYQTSARLRLENNFNRVYRYFEAFF
                     ERGRSPYIRFIRKNLTFLNTVIQNGDWNRLRRHPPCVVPDPRGEQHLMELALQRVRDW
                     GTRQTGSQRSLAEAS"
     gene            complement(4481..4870)
                     /locus_tag="VFL84_06715"
     CDS             complement(4481..4870)
                     /locus_tag="VFL84_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608350.1"
                     /translation="MRTLMAVLVAAAFAGTASAAKEPPATATYFDEAKITVNERASAD
                     GFIRVRVVPENGTALEATIPIRKRENENELARTITDGLNAVLAPSYGADKDGGEHVKI
                     KKKSRDAANFSVEITFNVPGFSIVLDK"
     gene            5011..6195
                     /locus_tag="VFL84_06720"
     CDS             5011..6195
                     /locus_tag="VFL84_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7608351.1"
                     /translation="MNRRDFVFAAASAALWPGAWPAAAQPRRYDLVIKGGRVIDPSLR
                     LDAVRDVGIAAGRIAAVEATIDATAAATLDARGKLVVPGLIDIHTHAAREAAGPALCL
                     RDGVTGFIDAGSAGADNLDPAVAIVKAAPQTARILVNIGRRGILPEGDAKDLALADVG
                     AARAAIERHRDYVVGVKARMTESVVGKNDVEVLKRTQQVAEPLGLRVMIHMGQSASPL
                     RELLPILKTGDIVTHMFAPPPNAIVDEAGRVLPEVLAARRRGIVFDVGNGVRDHIRWD
                     IVEQVMRQGFWPDTFSTDWNVMSRTTGVVDFPNCMSKLLGYGMSVPEAIACATVNAAR
                     TFEVFRDRGTLNVGAPADVAVLELREGEFEFVDNYDNKIKGRQRLFPSETVLAGARVP
                     RA"
     gene            6192..>6438
                     /locus_tag="VFL84_06725"
     CDS             6192..>6438
                     /locus_tag="VFL84_06725"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HET7608352.1"
                     /translation="MTMARPRAKFRSLRVTPEFRDFVLDQLAGVRELRSKRMFGGLGL
                     YAGERFFGIVAADELFFKVDDANRGAYEAAGSDAFRPL"
BASE COUNT         1036 a         2249 c         2113 g         1040 t
ORIGIN      
        1 gatgggcgcc ccgcccgtcg caatgacgat atgcggcgcg ctgaagcgct cgcccgtcgt
       61 ggtcacgagc tcggtcggac ttctgaacgc cgccgcgtcg cggatcgtca cgatgccctt
      121 cttgtcgagg ttgcgctcgt agatcccgtt caagcgcagc acgtatgcat cgcgctccag
      181 cttgagcttc gcccaatcgt ggccgccggt cttgacgtcg aacccgtaat acggcgcatg
      241 cgccagcgcg gcctgaagct ccgcggcgtt ccacatgacc ttcttcggca cgcagccgac
      301 gttgacgcag gtgccgccga gccgcgcggg ctcgaagata gcgacgcgcg cgccgtgctc
      361 ggccgcgcgc tgcgccgccg cgaggccgcc gctgccgccg cccaaaacca gcaaatcgaa
      421 ctgccgcacg cgccttcctt tcgctttggc accggcgcaa gagagtacag cagttgcgcg
      481 gccgaagcgg cgattggctc gtcggcgcag ccacgctaga gttcgtccat gaccgaaacg
      541 accgaaccgg gtccgaatcc gctgctcgcg gcgcgagtgc tgccggccta ttcggccgtg
      601 aagccggagc acgtcgagcc ggccgtgcgc gagctgctcg cggcgcagcg ccgcgccctc
      661 gtcgccgccg aaagcgtcgc gacgccggac ctcgactggc tgcgcaacct cgagcgcatc
      721 agcgtcgagc tcgaccgcgt ctggggcccc gtctggcact tgaactccgt cgtctcgact
      781 ccgccgctgc gcgacgcgtt caaccgttgc ctgccgctcg tgaccgagtt cggcaccgag
      841 ctcgcgcaga acgagacgct gtaccgccac ttcagcgcgc tgaaagcgag cgtcgggacg
      901 gcgaagccgg tcgagcggca gctcatcgac aacgcgttgc gcgacttcaa gctcggcggc
      961 gtcacgttga cgggcgcgcc gaagcagcgc ttccgcgagg tcatgcaaga gcttgccgcg
     1021 aagcaagcga agttcgagca gaacgtcatg gacgcgaccg atgcgttcga gcatcgcgag
     1081 acggaccccg ccgcgctcgc cgggctgccg cagttcgtgc aggaccgcgc cgcggcgctc
     1141 gcgaaagagc gcgatttcga gggctttagc ctgcggctcg atccgccgac ctatcagacc
     1201 gtcatggcgc acgccgaatc ggccgcgctg cgcgagaagt actaccgggc ctggaacacg
     1261 cgcgcgtcgg atcaagggca aaacccgcag tgggacaacg gcccgctcat cgacgagatc
     1321 ttggcgttac gccacgaggc ggcgcagctc gtcggcttca agacgttcgc ggaattttca
     1381 ctggcgacga aaatggccga gtcgccgcag cgcgtcatcg agttcctacg cgatctcgcg
     1441 cgccgcagca gagccgacgc gcgcgacgag ctcgccatgc tcaccgaata cgcgggccgc
     1501 aagctcgccg cctgggacgt tccgttctat gccgagcggc tgcggcaaga gaagctcggg
     1561 ctcgccgagg aagagctgcg cccctacttc gcgctgccgc gcgtgctcga cgggttgttc
     1621 gagctctgcg gcaggctgtt cgacatcgag gtcaagcaag cgccgccgcc cgatacctgg
     1681 cacgacagcg ttcgctacta cgagctgaaa cgccgcgacg gcacgctgat cggcagcttc
     1741 ttcaccgatc tctacgcacg gccgaacaag cgcggcggcg cctggatggg gggcgccgtg
     1801 agtcgcgcca agttgaacgg ccacgcgcag acgcccgtcg cgcatctcgt ttgcaacttc
     1861 aatgcgccgg ccgggacgac gccgtcgctg ctgacgcatt ccgaggtcgt cacgctgttc
     1921 cacgagttcg gccacgctct gcatcacctg ctgacggaag tcgactaccc gtcgctcgcg
     1981 ggcaccaacg gcgtcgcgtg ggacgcggtc gagctgccga gccagttcct cgagaacttc
     2041 gcgtggcggc ccgagatgct gaccgagatc ggccggcatt acgaaacggg cgcgccgctg
     2101 cccaacgaca agatcgagac gctgaagcgt tcgcgcacgt ttctgtcggg gctcgcaatg
     2161 gtgcgccagc tcgagttcgc gctgttcgac ttcaggctgc acgaggagta ctcggccgag
     2221 cgcggctcgc gcgtgcggga cgtgctcgac gaggtgcgcc gcgaggtcgc cgtcgtcgag
     2281 ccgccgccct tcaaccgctt cgcgcactcg ttcggccacg tcttcggcgg cggctacgcc
     2341 gcgggctact acagctacaa gtgggccgag gtgctcgcgg ccgacgcgtt cgcggcgttc
     2401 gaggaagccg gcgtgttcga ccgcgcgacg gccgagcgtt tcaggcgcgc ggtgctcgcg
     2461 acggggggca gccgcaaagc gctcgacgcg ttcgtcgatt tccgcggccg acagccaaag
     2521 ctcgatgcgc tgctcaagca atccggcatc gccgagcggc aatagcttct gaaagtcgca
     2581 acctggaaca tcaacagcct cagggtcagg ctgccccacg tcctgaagtg gctcgagcgc
     2641 gagcaaccgg acgtcttggc gctgcaggaa accaagattc agaacgacga gtttcccgcc
     2701 gacgcgttcg cgcagtacgg ctacgtcgcg gcgttcagcg gccagtcggc gtacaacggc
     2761 gtcgcgatct tgtcgcgcgc gccgctcgac gacatcacct acgatatcgg cgggggctac
     2821 actgatgagc aaaggcgcgt cctcggcgtg acggttcgcg gcgcgcgtct ttggagcctg
     2881 tacgtgccga acggccagag cgtcgactcg gaaaagtacc gctacaagct cgaatggctg
     2941 gcggcgctgc gcgcgcggct cgaggacgag atcgcgcggc atccaaagct gctcgtcgtc
     3001 ggcgacttca acgtcgcgcc cgacgaccgc gacgtccata accccgaggc ctggcgaggg
     3061 aagatcatgt gctcgccggc cgaacgcgcg gcgctgacgg gcctctgcgg cttaggctta
     3121 cgcgatgcat tccgcctgtt ctcgcagccc gagaaggtct tcagctggtg ggactaccgg
     3181 gccggggcat ttcgccgcaa ccaggggctc cgcatcgacc tcgtcctcgc ctcggatgca
     3241 ttaagtcaaa cgtgcagcgg ctgccgcatc gacaaggaac cgcgcggctg ggaccggccg
     3301 tcggaccacg tgcccgtgat cgcagaattc gcgctccctt agcccttatg tgacatgcac
     3361 gatactggcg gctcggcgaa cctcttagac ttatcggcct ggttggaaga cccgatgccg
     3421 atagaagacg agctacaaga ccggcgcaac gactacgacg tcaccaacac cgtgcaagtg
     3481 agctcctcac ggcatgtgcg cgccgctgtt gccgacgttt tcgctgccct gtacccgagc
     3541 agctcgttcg attcggtctg gatggcgttc cacgacttcg agcgcgtgtt ccgcggcctc
     3601 gacccgaact accacgccgt cgacacgacc tatcacgaca tgcagcacac gctcgacatg
     3661 acgctcgcgc tcgcgcggct catcgcgggc cacgaaatga gcgtcgagcc ggccgaccgt
     3721 ctcggcgccg agcgcgccga gctcgcgatc gtcagcgcct tgtttcacga cgtcggttat
     3781 ctgcggcaca aggaccgcga ccacgcggcc gtcaacggcg ccgtgttcac gcgcgtgcac
     3841 gtgtcccgca gcggccacta cctcgagaag tacttgccgc gcatcggcct cgagcagttc
     3901 gtgcccgtcg tggcgcgcat cgtccacttc accggctacg agctcaagct cgatcagatc
     3961 gagctcgagc agccgaagga cagcgtcgtc ggccacctgc tcggcacggc cgacctcgtc
     4021 gcgcagctcg ccgaccgttg ctatctcgag aagtgccgcg accgccttta ccccgagttc
     4081 gtgctcggcg gcgtcgcgat cgacgagcgg cccgacggcg cggtgctcta tcgctcggcc
     4141 gaggacctgc tcggcaagac gctgtcgttc taccagactt cggctcgctt gcgcctcgag
     4201 aacaacttca accgcgtcta tcgctatttc gaggcgttct tcgagcgcgg ccgcagcccg
     4261 tacatccgct tcatccgcaa gaacctgacg ttcttgaaca ccgtcattca gaacggcgat
     4321 tggaaccggc tgcgccggca cccgccctgc gtcgtgccgg acccgcgcgg cgagcagcac
     4381 ctcatggagc tcgcgctgca gcgcgtgcgc gactggggca cgaggcagac cggctcgcag
     4441 cgctcgctcg cggaagccag ctgattcttg ggcaacgcct ctacttgtcg aggacgatcg
     4501 agaagcccgg cacgttgaac gtgatctcga ccgagaaatt ggccgcgtcg cgcgacttct
     4561 tcttgatctt gacgtgctcg ccgccgtcct tgtcggcccc gtagctaggc gccagcacgg
     4621 cattgagccc gtccgtgatc gtgcgcgcga gctcgttctc gttctcgcgc ttgcggatcg
     4681 ggatcgtcgc ttcgagcgcg gtgccattct ccggcacgac gcgcacgcgg atgaagccgt
     4741 cggcgctggc gcgctcgttg accgtgatct tcgcttcgtc gaagtaagtc gccgtcgcgg
     4801 gaggctcctt tgcggccgaa gccgtgcccg cgaacgcagc cgccaccagc accgccatca
     4861 gggttctcat cgtcctcacc gccgttggtc ctcgagaatg ccggccaatc tagccggcgc
     4921 atccgggcca aactacggcc tgcttctcgc tttcgccttc taccgctcag gtccgctaag
     4981 ctcttgaccg ggccgaaggg ggacgcggca atgaatagac gggacttcgt gtttgcggcc
     5041 gcgagcgcgg ccctgtggcc gggcgcctgg cccgctgcgg cgcagccgcg ccgctacgat
     5101 ctcgtcatca aaggcggccg cgtcatcgac ccgtcgctgc ggctcgacgc cgtgcgcgac
     5161 gtcggcatcg ccgccggccg catcgcggcc gtagaggcga cgatcgacgc cacggcggcc
     5221 gcgacgctcg acgcgcgcgg caagctcgtc gtgccgggct tgatcgacat ccacacgcac
     5281 gccgcccgcg aggcggcggg cccggcgctg tgcttgcgcg acggcgtcac gggtttcatc
     5341 gacgcgggct cggccggcgc cgacaatctc gaccccgccg tcgcgatcgt caaagccgcg
     5401 ccgcagacgg cgcgcatcct cgtcaacatc ggccgccgcg gcatcctgcc cgaaggcgac
     5461 gccaaggacc tcgcgctcgc cgacgtcggc gccgcgcggg ccgcgatcga gcggcatcgc
     5521 gactacgtcg tcggggtcaa ggcgcgcatg accgaaagcg tcgtcggcaa gaacgacgtc
     5581 gaggtcctga agcggacgca gcaagtggcc gagccgctcg ggctgcgcgt catgattcac
     5641 atggggcaga gcgcgtcgcc gctgcgcgag ctgctgccga tcttgaagac cggcgacatc
     5701 gtgacgcaca tgttcgcgcc gccgccgaac gcgatcgtcg acgaggccgg ccgcgtgttg
     5761 cccgaggtcc tcgcggcccg ccggcggggc atcgtgttcg acgtcggcaa cggcgtgcgc
     5821 gatcacattc gctgggacat cgtcgagcaa gtgatgcgtc agggcttctg gcccgacacg
     5881 ttctcgacgg actggaacgt catgagccgc acgacgggcg tcgtcgactt tccgaattgc
     5941 atgtcgaagc tgctcggcta cggcatgtcg gtgccggagg cgatcgcttg cgcgaccgtc
     6001 aacgcagcac gcacgttcga ggtcttccgc gaccgcggca cgctgaacgt cggcgctccg
     6061 gccgacgtcg ccgtcctcga gctgcgcgaa ggcgagttcg agttcgtcga caactacgac
     6121 aacaagatca agggccggca gcgcctcttt ccgagcgaga ccgtgctcgc gggcgcgcgc
     6181 gtgccgcgcg catgacgatg gcgcgcccgc gcgccaagtt tcgctcgctg cgcgtgacgc
     6241 cggagtttcg cgacttcgtg ctcgatcagc tcgccggcgt gcgcgagctg cgctcgaaaa
     6301 gaatgttcgg cggcctcggt ctgtacgccg gcgagcgctt cttcggcatc gtcgcggccg
     6361 acgagctttt cttcaaggtc gacgacgcga accgcggcgc ctatgaagcc gccggcagcg
     6421 acgcgttccg gccgttgg
//
LOCUS       DASSNN010000367         3380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_300467, whole genome shotgun sequence.
ACCESSION   DASSNN010000367 DASSNN010000000
VERSION     DASSNN010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300467"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VFL84_06730"
     CDS             complement(<1..900)
                     /locus_tag="VFL84_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HET7608353.1"
                     /translation="MSSRYDAAKARARRGRVGAAVYAAVLVAAFLPNSHAQEAESPAL
                     RYERAQAFARSGSPEAALVELDALLAQFPNDADYLLGRAQMLARLGQDAAAVETAERA
                     LRLAPDYEDVWQLRLRLAERAGDDAAVAALRAQVAARFPDASWWHAPPAPIEYRRWLS
                     AGSGTDRLSNGAPDWSREHLRLDFNTADGGALFAELSRSERFDEADSALYVGGSWGAL
                     RHWQLGAAAALTEDPRFLPERELSVDAARGWARGWGSAFAVRERSYATGDVTTYSFTG
                     DKYVSDYRIAYRLDHSRQTGADSA"
     gene            complement(897..1331)
                     /locus_tag="VFL84_06735"
     CDS             complement(897..1331)
                     /locus_tag="VFL84_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010628284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7608354.1"
                     /translation="MLRTVNRGMGEQAVETKPERVLVVEDNEHAAYLLKTLLERAGYS
                     VIVSKDGRDALAKLKSMEPVDLVMLDLMLPYVSGYQVLIEARQNPKWRRVPIVVVTAR
                     TLEMDAVRALETGANDFVRKPFAPEELLARARRAIELQRGAP"
     gene            complement(1479..>3380)
                     /locus_tag="VFL84_06740"
     CDS             complement(1479..>3380)
                     /locus_tag="VFL84_06740"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET7608355.1"
                     /translation="PEGLPAILSAVLAVGVQRMAKSNAIVKDLASVETLGSASVICSD
                     KTGTLTRSEMTIKRVMTASGGTRVTGVGYAPEGRVEHEGAPASGPVLAEHIVVLSGGT
                     LASNADLRETSSGVWEIQGDPTEAAFLVAERKLGAAERRERRFERVREFPFTSDRKMM
                     STIALDHEHGGAPILISKGAPDVLLERCTRVREGMEAVPLDDARRRKILADVDALSDE
                     ALRTLSVAYRPLAASEDPAEGEVPERDLIFVGTVGIIDPPREEAGVAIREAQRAGIRV
                     VMITGDHPRTAARIAADLGIVERGEPALTGRELDALDGAAFAAAVRKTSVYARVAPAH
                     KLKIVDALQADGHVVAMTGDGVNDAPALKSADIGVAMGIAGTEVAKEAAKMILADDNF
                     ATIVEAVRAGRVIFTNIRKFLRYLLASNMGEVLTVFAGVVLARAIGLGTGGDGLALPL
                     LATQILWINLVTDSAPALAMGLDPPSDDVMSYPPRRPNDRVIDGRMWAGVVAIGAAMA
                     AFTLLTIDLYLPGGLIEGDRSLDNARTAGFTVLVLAQLFNCLNARSDRVSALRGAFRN
                     AWLWSALALSAALQVAVVNVSALNHAFGTVPLGLDQWLVCIAMASGVLWVSELRKLIG
                     FAIARAARR"
BASE COUNT          507 a         1235 c         1136 g          502 t
ORIGIN      
        1 agccgaatcg gcgcccgtct ggcgcgagtg gtcgagccgg tacgcgatgc gatagtcgga
       61 gacgtacttg tcgcccgtga acgagtacgt cgtcacatcg ccggtcgcat agctgcgctc
      121 gcgcacggcg aaggcgctcc cccagccgcg cgcccagccc cgggccgcgt cgaccgagag
      181 ctcgcgctcg ggcagaaagc gcgggtcctc ggtgagggcg gcggctgcgc cgagctgcca
      241 atgccgcagc gcgccccagc tgcccccgac gtacagcgcg ctatcggcct cgtcgaatcg
      301 ctcgctgcgc gagagctcgg cgaacagcgc tccgccgtcg gcggtgttga agtcgagacg
      361 cagatgctcg cggctccaat cgggcgcgcc gttcgacaga cgatcggtgc ccgagcccgc
      421 cgagagccaa cgccggtatt cgatcggcgc gggtggcgcg tgccaccagc tcgcgtcggg
      481 gaagcgcgcg gcgacttgcg cgcgtagcgc ggcgacggcc gcgtcgtcgc cggcccgctc
      541 ggcgagcctc aaccgcagct gccagacgtc ctcgtagtcg ggcgcgagcc gaagcgcgcg
      601 ttccgcggtc tcgacggccg cggcgtcttg accgagccgc gccagcatct gcgcacggcc
      661 gagcaagtag tcggcgtcgt tcggaaactg cgcgagcagc gcatcgagct cgacgagcgc
      721 ggcctccggc gagcccgagc gcgcgaacgc ctgagcacgc tcgtaacgca gcgcggggct
      781 ctcggcttct tgcgcatgag aattgggcag gaaggcggcg acgagcacgg ccgcgtaaac
      841 cgcggcgccg acgcgccctc gccgagcgcg agccttcgcc gcgtcgtagc gcgagctcac
      901 ggcgcgcccc gctgcagctc gatcgcgcgc cgcgcgcgcg cgagcagctc ttcgggcgcg
      961 aacggcttgc gcacgaagtc gttggctccc gtctcgagcg cgcgcacggc atccatctcc
     1021 aacgttcgcg cggtcacgac gacgatcggc acgcgccgcc acttcgggtt ctgccgcgcc
     1081 tcgatcagca cctggtaacc cgacacgtac ggcagcatga gatcgagcat gacgagatcg
     1141 accggctcca tcgacttgag cttcgcgagc gcgtcgcggc cgtccttgct cacgatgacg
     1201 ctgtagccgg cccgctcgag tagcgtcttc agcaaatacg cggcatgctc gttgtcctcg
     1261 acaacgagca ctcgttcggg ctttgtctcg acggcttgct cgcccattcc gcggtttacg
     1321 gtccgcaaca ccgccagcca ggttatgtcg actctcggaa tcgcgcggtg acggcgctcc
     1381 cagaatcggc agttagcccc gagaccggcg cgcttcgcgg gaccgatttc cgagccgttt
     1441 cgagggggcc aaagcgagcg ccggcgcggg ttgcgggcct agcggcgcgc cgcgcgcgcg
     1501 atcgcgaacc cgatcagctt gcgcagctcg ctgacccaga gcacgccgct cgccattgcg
     1561 atgcagacga gccactgatc gaggccgagc ggcaccgtgc cgaatgcatg gttcagcgcg
     1621 ctcacgttca cgacggcgac ttgcagcgcc gcggacagcg cgagcgcgct ccacagccac
     1681 gcattcctga acgcgccgcg cagcgcgctc acgcggtcgg agcgcgcgtt caagcagttg
     1741 aacagctgcg cgagcacgag caccgtgaag cccgccgtgc gcgcgttgtc gaggctgcga
     1801 tctccttcga tcaagccgcc cggcagatag aggtcgatcg tcagcagcgt gaatgccgcc
     1861 atcgccgcgc cgatcgcgac gacgccggcc cacatgcgcc cgtcgatgac gcggtcgttc
     1921 ggccggcgcg gcgggtagct catcacgtcg tcgctcggcg gatcgaggcc cattgcgagc
     1981 gccggcgccg agtccgtgac gaggttgatc cagagtatct gcgtcgcgag caacggcagc
     2041 gcgaggccat cgcctccggt gccgaggccg atcgcgcgcg cgagcacgac gccggcgaac
     2101 acggtcagga cttcgcccat gttcgacgcc aacaggtagc gcaagaactt tctgatgttc
     2161 gtgaaaatca cgcggcccgc acgcacggct tcgacgatcg tcgcgaagtt gtcgtccgcg
     2221 agaatcatct tcgcggcctc cttcgcgacc tcggtgcccg cgattcccat cgcgacgcct
     2281 atgtcggcgg acttcaaggc cggcgcgtcg ttgaccccgt cgcccgtcat tgcgacgacg
     2341 tgcccgtcgg cttgcagcgc gtcgacgatc ttgagcttgt gcgcgggcgc gacgcgcgca
     2401 tacaccgaag tcttgcggac cgcggccgcg aacgccgcgc cgtcgagcgc gtcgagctct
     2461 cggcctgtga gcgccggttc cccccgctcg acgatcccga ggtccgcggc aatccgcgca
     2521 gccgtgcgcg ggtggtcgcc ggtgatcatg accacgcgaa tgccggcgcg ctgcgcctcg
     2581 cgaatcgcga cgccggcttc ctcgcgcggc ggatcgatga tgccgacggt gccgacgaaa
     2641 atcaggtcgc gctccggcac ctcgccttcg gccgggtcct cgctcgccgc gagcggccga
     2701 tacgcgaccg acagcgtgcg caacgcctcg tccgacagcg cgtcgacgtc ggcgagtatc
     2761 ttgcgccgcc gcgcgtcgtc gagcggcacg gcttccatac cctcgcgcac acgcgtgcag
     2821 cgctcgagca gcacgtcggg agcccccttg ctgatcagga tcggcgcgcc gccgtgctcg
     2881 tgatcgagcg cgatcgtcga catcatcttg cggtcggacg tgaacgggaa ctcgcgcact
     2941 cgctcgaagc gccgctcgcg ccgctcggcg gcgccgagct tgcgctccgc cacgagaaac
     3001 gccgcttcgg tcggatcgcc ttgaatctcc cataccccgc tcgacgtctc gcgcagatcg
     3061 gcgttgctcg cgagcgtgcc gccgctcaac accacgatgt gctccgccag caccggcccg
     3121 gacgccggcg ctccctcgtg ctcgacgcgt ccttccggcg cgtaaccgac gcccgtgaca
     3181 cgcgtgccgc cggacgccgt catcacgcgc ttgatcgtca tctcggagcg cgtcagcgtg
     3241 ccggtcttgt ccgagcaaat cacggaggcc gagccgagcg tctcgacgga cgcgagatcc
     3301 ttcacgatcg cgttgctctt tgccattcgc tgcacaccga ccgcgagcac ggcggacagg
     3361 atcgcgggca ggccctcggg
//
LOCUS       DASSNN010000368         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1579020, whole genome shotgun sequence.
ACCESSION   DASSNN010000368 DASSNN010000000
VERSION     DASSNN010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579020"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="VFL84_06745"
     CDS             <1..1272
                     /locus_tag="VFL84_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7608356.1"
                     /translation="NTAVLAALLDAGAEVDAPGADGQTPLMVVARSSNTAAAEVLIAN
                     GADVNARERWRQQTALMWAAAQSQPAMVDLLVANGAEVDARSAVNDWPRQVTGEPRRM
                     YRPFGGLTPLLFAAREGCLDCVRSLVAAGANVDLPDPKGVTPLFLAIDNFHFDTAKLL
                     IEAGANPNKWDWWGRTPLYSAVDVNTMPHGGRADRRSTDETTSTEIIELLLEAGAWPN
                     VQLKLLPPYRHIGDDRGCDTMLVTGTTPLLRAAKTFDTAAIELLLEHDANVDLPNDGG
                     VTPVMAAAGVGSVECDPRGYGPGIPHYLAPETEQRSIAALKLLLDAGANIDARTLSGR
                     AGARSPGRTALFGAAFWGWSDVVDYLVERGAKIDVAERGGLTPVDAALGRAGGHGRGS
                     TIEVYEDTAARLRELCQQQKDCDLSRPQGPS"
     gene            1294..>2048
                     /locus_tag="VFL84_06750"
     CDS             1294..>2048
                     /locus_tag="VFL84_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7608357.1"
                     /translation="MSAIACLRASLAFAILLMSGYAAAQRQLPPMSIEQLTPHVYRYG
                     GLTNGAFVVGSEGIAVIDGQICGSNGTQWLKDELKKRFPALPVKYTILSHDHEMHICG
                     LEVFSDTARAISHVNAKGHIIREKRRTVVPEITFDERMSIDLGGIEVVMFYLGPTHTD
                     NLIQVHVPSEKVLIAVDFVREGKSLAMPELRDLSLDNSIRALGYLGRMEDVDIVVPGH
                     GGVTTQQSFLYVRDFLVALKERVLEQMVAGKGI"
BASE COUNT          341 a          725 c          682 g          300 t
ORIGIN      
        1 aacaccgcgg tcttggccgc cctgctcgat gccggcgccg aagtcgacgc gcccggcgcc
       61 gacggccaga ccccgctcat ggtcgtcgcg cgcagcagca acacggcggc ggccgaggtg
      121 ctgatcgcga acggcgccga cgtcaatgcg cgcgagcgct ggcggcagca gacggcgttg
      181 atgtgggccg ccgcgcagag ccagcccgcg atggtcgacc tgctcgtcgc caacggcgcc
      241 gaggtcgacg cgcgctcggc cgtcaacgat tggccgcgcc aagtcacggg cgagccgcgc
      301 cgcatgtacc ggccattcgg cggcctcacg ccgcttctgt ttgcggcacg cgaaggctgc
      361 ctcgactgcg tgcgctcgct cgtcgcggcc ggcgcgaacg tcgatctccc cgatccgaaa
      421 ggcgtcacgc cgctgttcct cgcgatcgac aacttccact tcgacaccgc gaagctgctc
      481 atcgaggccg gcgcgaaccc gaataagtgg gactggtggg gccgcacgcc gctctactcg
      541 gccgtcgacg tcaacacgat gccgcacggc ggacgcgccg atcggcgctc gaccgacgag
      601 acgacgagca ccgagatcat cgagctcctg ctcgaagcgg gcgcctggcc caacgttcag
      661 ctcaagctgc tgccgccgta ccgccacatc ggcgacgacc gcggctgcga cacgatgctc
      721 gtgaccggca ccacgccgtt gctgcgagcg gccaagacct tcgacaccgc cgcgatcgag
      781 ctgctgctcg agcacgacgc gaacgtcgac ttgccgaacg acggcggcgt cacgcccgtc
      841 atggcggcgg ccggcgtcgg ctccgtcgaa tgcgacccgc gcggctacgg ccccggcatc
      901 ccgcactacc tggcgcccga aaccgagcag cgatcgatcg cggcgctgaa gctgctgctc
      961 gacgcgggcg cgaacatcga cgcgcgcacg ctgagcggcc gcgccggggc caggagcccc
     1021 ggccgcacgg cgctgttcgg cgccgcgttt tggggctgga gcgacgtcgt cgactacctc
     1081 gtcgagcgcg gggcgaaaat cgacgtcgcc gagcgcggcg gcttgacgcc cgtcgacgcg
     1141 gccctgggcc gcgcgggcgg ccacggccgc ggctcgacga tcgaagtcta cgaggacacg
     1201 gccgcgcgct tgcgcgagct gtgccagcag caaaaagact gcgatttgtc tcggccgcaa
     1261 ggcccatcct agcgagaccg attggggggt tcgatgagcg ccattgcatg tctgcgagcg
     1321 tcgctcgcat tcgcgatcct tttgatgagc ggttacgcgg cggcgcaacg gcaactgccg
     1381 ccgatgtcga tcgagcagct cacgccgcac gtctaccgct acggcggctt gacgaacggc
     1441 gccttcgtcg tcggctccga aggcatcgcc gtcatcgacg ggcaaatttg cggcagcaac
     1501 ggcacgcagt ggctcaagga cgagctcaag aagcgtttcc ccgccctgcc cgtcaagtac
     1561 acgatcctga gccacgatca cgagatgcac atctgcgggc tcgaggtctt cagcgacacg
     1621 gcgcgcgcga tctcgcacgt caacgcgaag ggccacatca ttcgcgagaa gcgccgcacc
     1681 gtcgtgcccg agatcacgtt cgacgagcgc atgtcgatcg acctcggcgg catcgaggtc
     1741 gtgatgttct acttgggccc gacgcacacg gacaacctga ttcaagtgca cgtcccttcc
     1801 gagaaggtgc tgatcgccgt cgacttcgtg cgcgaaggca agagcctcgc gatgccggag
     1861 ctccgcgact tgagcctcga caactcgatc cgcgctctcg gttacctcgg gcgcatggag
     1921 gacgtcgaca ttgtcgtgcc gggccacggc ggcgtcacga cgcagcagtc gttcctctac
     1981 gtgcgcgatt tcctcgtcgc gctcaaggag cgcgtgctcg agcagatggt tgcgggcaaa
     2041 ggcatcga
//
LOCUS       DASSNN010000369         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_196014, whole genome shotgun sequence.
ACCESSION   DASSNN010000369 DASSNN010000000
VERSION     DASSNN010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196014"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /gene="nuoG"
                     /locus_tag="VFL84_06755"
     CDS             complement(<1..1662)
                     /gene="nuoG"
                     /locus_tag="VFL84_06755"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HET7608358.1"
                     /translation="MSTPAPTAPPPAAPPADPNVVNVEVNGVPLKARKGQMIIEVTDK
                     AEIYVPRFCYHPKLTIAANCRMCLVEVEKAPKPLPACATPVAEGMKIFTRSKRAISAQ
                     KATMEFLLINHPLDCPICDQGGECELQDLAMGFGRDVSRYTERKRIVKDKNIGPLVST
                     DMTRCIHCTRCVRFGAEIAGIQELGANFRSDRSEIGTYIEMSVDHELSGNIIDLCPVG
                     ALNNKPYRYSARAWEMVAKPLVSPHDCAGSNLFGHVLRGQVRRVVPRENEAINETWIS
                     DRDRFSCHALYGEDRLRTPRVKHEGEWVEATWHDALAAAVGTLREATAGGGASGLGTL
                     VSPSATLEEAYLLQRITRHLGSENIDYRLRQRDFRDQASDARAPLLGCSLADLDTRQG
                     MLVVGSNLRMEVPIVAHRIRKAARKGASVAFVNTAEYEYYFKPAAYLTAPPEALAADL
                     AGVLAAAALAAGTALPAPLARAVEGAAVTDAHHAAAQALTRKPALVLLGHIAQRHPQY
                     ADLRALAAGLAAVTGATLGYLSEGANAAGAALAGATPHRGPGGKPA"
     gene            complement(1659..>2603)
                     /gene="nuoF"
                     /locus_tag="VFL84_06760"
     CDS             complement(1659..>2603)
                     /gene="nuoF"
                     /locus_tag="VFL84_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017925961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET7608359.1"
                     /translation="TVAYNYIRGEFMAEPIPRFEQALKEAYAAGLLGKNIRGSGVDMD
                     IHTFVGAGAYICGEETALLESLEGKQGKPRFKPPFPANFGLYGAPTTINNTQSVASIP
                     TILRKGSQWFADLGVKGSGGTAIYSVSGHVEKPGNYELPLGIPFPELLEIAGGVRGGR
                     KLKAVIPGGSSCPVIPAEKMMTLTMDFDTVKAAGSGLGTCATIVMDDTTCMVKMLRRI
                     SRFYHAESCGQCTPCREGTGWLHRVLTRIVEGKGTKADIDMLVDVANKIEGHTICAFG
                     DAAAWPVQSFIKHFRHEFEYMVEHKGRSIVDDLPSRAA"
BASE COUNT          374 a          876 c          897 g          456 t
ORIGIN      
        1 ggcgggctta ccgcccggcc cacgatgcgg cgtcgcgccg gcgagcgcgg cgcccgcggc
       61 gtttgcgccc tcgctcaagt agccgagcgt cgcgccggtc acggccgcga ggcccgcggc
      121 gagcgcgcgc aagtcggcgt attgcggatg gcgctgcgcg atgtggccga gcagcacgag
      181 cgcgggcttg cgcgtgagcg cttgcgccgc cgcgtgatgc gcgtccgtga cggcggcgcc
      241 ttcgacggcg cgcgcgagcg gcgccggtag cgccgtgccc gccgctaaag cggccgccgc
      301 gagcacgccg gcgagatcgg cggccagcgc ttcgggcggc gcggtcagat acgctgccgg
      361 cttgaagtag tactcgtatt cggccgtgtt cacgaacgcg acgctcgcgc ctttgcgagc
      421 cgccttgcgg atgcggtgcg cgacgatcgg cacttccatg cgcaggttcg agcccacgac
      481 gagcatgccc tgtctagtgt cgagatccgc gaggctgcaa ccgagcagcg gcgctcgcgc
      541 atcgctcgct tgatcgcgga agtcgcgttg ccgcaagcgg taatcgatgt tctcgctgcc
      601 caagtggcgc gtgatgcgct gcagcaggta tgcctcctcg agcgtcgcgc tcggggaaac
      661 gagcgtgccg agcccgcttg cgccgccgcc cgcggtggcc tccctgagcg tgccgacggc
      721 cgcggccagc gcgtcatgcc acgtcgcttc gacccactcg ccctcgtgct tgacgcgcgg
      781 cgtgcgcagc cggtcctcgc cgtagagtgc gtggcagctg aagcggtcgc gatcggagat
      841 ccaggtttcg ttgatcgcct cgttctcgcg cggcacgacg cggcgaacct ggccgcgcag
      901 cacgtgcccg aacaagttgc tgccggcgca gtcgtgcggg ctcacgagcg gcttcgcgac
      961 catctcccag gcgcgcgcgc tgtagcgata cggcttgttg ttgagcgctc cgacggggca
     1021 cagatcgatg atgttgccgc tgagctcgtg atcgacactc atctctatat aggtgccgat
     1081 ctcggagcgg tcgctgcgga aattcgcgcc gagctcttgg atgccggcga tctcggcgcc
     1141 gaaccggacg cagcgcgtgc agtgaatgca gcgcgtcatg tcggtcgata cgagcggacc
     1201 gatgttcttg tccttgacga tgcgcttgcg ctccgtgtag cgagacacgt cgcggccgaa
     1261 gcccatcgcg agatcctgca gctcgcactc gccgccttga tcgcagatcg ggcaatcgag
     1321 cggatggttg atgagcaaga actccatcgt ggctttctgc gccgagatcg cgcgcttcga
     1381 ccgcgtgaag atcttcatgc cctcggcgac cggcgtcgcg caggccggca gcggcttcgg
     1441 cgccttctcg acctcgacca ggcacatgcg gcagttcgcc gcgatcgtga gcttcgggtg
     1501 atagcagaac cgcggcacgt agatctcggc cttgtccgtc acctcgatga tcatctggcc
     1561 cttgcgcgcc ttcagcggca cgccgttgac ctcgacgttg acgacgttcg ggtcggccgg
     1621 tggggccgca ggcggcggcg cggtgggtgc cggcgtgctc atgcggccct cgacggcaaa
     1681 tcgtcgacga tgcttctgcc cttgtgctcg accatgtact cgaactcgtg gcggaagtgc
     1741 ttgatgaagc tctgcacggg ccacgcggcg gcgtcgccga acgcgcaaat cgtgtggcct
     1801 tcgatcttgt tcgcgacgtc gacgagcatg tcgatgtcgg ccttcgttcc cttgccctcg
     1861 acgatgcgcg tcagcacgcg gtgcagccag cccgtgccct cgcggcacgg cgtgcactgc
     1921 ccgcacgact ccgcgtgata gaagcgcgag atgcggcgca gcatcttgac catgcaggtc
     1981 gtgtcgtcca tgacgatcgt cgcgcaggtg ccgagacccg acccggccgc cttgaccgtg
     2041 tcgaagtcca tcgtcaacgt catcatcttc tcggccggga tcacggggca cgacgagccg
     2101 ccgggaatga ccgctttcaa cttgcggccg ccgcgcacgc cgcccgcgat ctcgagcagc
     2161 tccgggaacg gaatgccgag cggcagctcg tagttgccgg gcttctcgac gtggcccgag
     2221 acggagtaga tcgcggtgcc gccggagcct ttgacgccga gatcggcgaa ccactgcgag
     2281 cccttgcgca agatcgtcgg aatcgacgcg acgctctgcg tgttgttgat cgtcgtcggc
     2341 gcgccataca ggccgaagtt cgccgggaac ggcggcttga accgcggctt gccctgcttg
     2401 ccttcgagcg actcgagcag cgccgtctct tcgccgcaga tgtaggcgcc ggcgccgacg
     2461 aaggtatgga tgtccatgtc tacgccggag ccgcggatgt tcttgccgag cagcccggcc
     2521 gcgtacgctt ccttgagcgc ctgctcgaag cgcgggatcg gctcggccat gaactcgccg
     2581 cggatgtagt tgtaggcgac cgt
//
LOCUS       DASSNN010000370         2990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_67617, whole genome shotgun sequence.
ACCESSION   DASSNN010000370 DASSNN010000000
VERSION     DASSNN010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67617"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFL84_06765"
     CDS             complement(<1..469)
                     /locus_tag="VFL84_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011935926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7608360.1"
                     /translation="MGAAIEIEARAASAAPWTLRRVVTAGVIGNVLEWYDFAVYGYFA
                     SILGEKFFPDADPRVSLLASFVTFAVGFLMRPVGAAIFGHIGDRYGRARALFLSIAMM
                     AVPTVLMGLLPTYQTIGIAAPVLLVALRMCQGIAVGGEFTASIVFLAENAPAHR"
     gene            638..1633
                     /locus_tag="VFL84_06770"
     CDS             638..1633
                     /locus_tag="VFL84_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608361.1"
                     /translation="MSRVAAAVGVLASWMGTSAAWAQSGEYAAPRTSFGHPDLSGTWT
                     NNNATPLQRPAQWADKELLTETELAAVRSAARHLEADGDALFGDELILDAISGDEESE
                     SHDTETGNYNGFWLPPRDFERRTSLIIDPPNGRIPAYTPEAQARRAAAAEQRRLHPAD
                     GPWSRGLGERCITFGVPRLTAAYSSVFEVVQSPYEVVFVMETIHNARVIPLDGRPHLP
                     ASLPQWDGSSRGHWEGDTLVVDTIGFSAKSDMLGSSTGLHLVERFTRIAPGVLQYDVT
                     MSDPSTWTQPWTVRIPMKQIDEPLIEYACHEGNLGMVGILAGARKQEADAARAHK"
     gene            1662..2891
                     /locus_tag="VFL84_06775"
     CDS             1662..2891
                     /locus_tag="VFL84_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7608362.1"
                     /translation="MKRPLVTLLAFVCSSLAASAACAQSLVVTNARILDGAGGEIANG
                     TIVVEGGVIRSVGAGAAPRGSANVVDAGGRTVMPGFIDAHRHVGAAEPALMKSFIDAG
                     FTTVLDALSDVPALVAVRNKIDAGEMVGPRLKISGLVPLNQTALPGGVDPARRDPARI
                     PFNQRPPVDCTAPDQARGMVNRIADQGGDNIKIILVASPKNCDLATLRAISDEAHKRG
                     LRMITHNTAVVDAVMAADAKTDYFAHTPHIGWVDEGDAIRKIVAAGAPMVTTLGVFTP
                     YFDDTHHDGLFRDNNEFQWDALHSAGQGPVNDRFLWNAGVVIGYGTDTSFAPRDSLRH
                     ELRALSSVFGPKELVTILTKNAAICADVDEITGTIETGKQADLVMLDGGDPLKDIVAV
                     LDVRLVVKGGKIVSDRR"
BASE COUNT          527 a          999 c         1007 g          457 t
ORIGIN      
        1 ggcggtgcgc cggcgcgttc tcggccagga acacgatcga ggccgtgaat tcgccgccga
       61 cggcgatccc ttggcacatg cgcagtgcca cgagcaacac cggagctgcg atcccgatcg
      121 tctgataggt cgggagaagg cccatgagca cggtcggcac ggccatcatc gcgattgaaa
      181 ggaatagcgc gcgagcccga ccgtagcggt ctccgatgtg gccgaagatc gccgcaccga
      241 cgggccgcat caggaatccg accgcgaacg tcacgaatga agcgagcaac gagacgcgcg
      301 ggtccgcgtc ggggaaaaac ttctcgccga ggatcgaggc aaagtacccg tacacggcga
      361 agtcgtacca ctcgagcacg ttgccgatca cgccggcggt cacgacgcgc cgtagtgtcc
      421 acggcgcggc gctcgcggcg cgcgcctcga tctcgatggc tgcccccatg ccgcgcgagt
      481 gtacctgttc ctgtgttgcg gcgccgccgc ggcgcgatgc gcgacactac cgtccgttcg
      541 agtttgatcg cggcaggaat cgaggcatac agtatcggcg gcgccgccga agacggagca
      601 gacgatgcca acgcacgaaa ctcgtacgtg ccgaaccatg agccgtgtcg cggccgccgt
      661 cggcgtcctc gcctcttgga tggggacctc ggccgcgtgg gctcagtcgg gcgagtacgc
      721 ggcgccgcgg acttcgttcg gtcaccccga cctctcgggc acttggacga acaacaatgc
      781 gacgccgctg cagcgccccg cgcagtgggc cgacaaggag ctcctgaccg agaccgagct
      841 cgcggccgtg cgcagcgcgg cgcggcacct cgaagcggac ggcgacgcct tgttcggcga
      901 cgagctgatt ctcgacgcga tctccggaga cgaggaatcc gagtcgcacg acaccgagac
      961 cggcaactac aacgggttct ggctgccgcc gcgtgacttc gagcggcgga cctcgctgat
     1021 catcgacccg ccaaacgggc gcatcccggc gtacacgccc gaagcgcaag cgcggcgcgc
     1081 cgcggccgcg gagcagcggc gcttgcaccc cgccgacgga ccgtggagcc gcggcttggg
     1141 cgagcggtgc atcacgttcg gcgtgccgcg cctcaccgcg gcgtacagca gcgtgttcga
     1201 agtcgtacag tcgccgtacg aggtcgtctt cgtcatggag acgattcaca acgctcgcgt
     1261 gattccgctc gacgggcggc cgcatctacc ggccagcctt ccgcaatggg acggcagctc
     1321 gcgcgggcat tgggaaggag acacgctcgt cgtcgacacg atcggcttct ccgcgaagag
     1381 cgacatgctg ggatcgtcga ccggtctgca cctcgtcgag cgcttcacgc gcatcgcacc
     1441 cggtgtgctg cagtacgacg tgacgatgag cgacccctcg acgtggactc agccctggac
     1501 ggtgcgcatt ccgatgaagc agatcgacga gccgctgatc gagtacgctt gtcacgaagg
     1561 gaatctcggc atggtcggca ttctggccgg tgcccgcaaa caagaagcgg acgccgcgcg
     1621 cgcacacaaa tagtcttttc tagctcggag atacaacaac catgaagcgt ccgctcgtaa
     1681 ctctcttggc attcgtctgc agctcgctag ccgcgagcgc ggcgtgtgcg cagagcttgg
     1741 tcgtcacgaa tgcgcgcatc ctcgacggag cgggcggcga gatcgcgaac ggcaccatcg
     1801 tcgtcgaagg cggtgtgatt cgctccgttg gcgccggcgc tgcgccgcgc ggttccgcca
     1861 acgtcgtcga tgccggcggc cgcacggtga tgccgggctt catcgatgcg catcggcacg
     1921 tcggcgccgc cgagccggcg ctcatgaagt cgttcatcga cgccgggttc acgaccgtgc
     1981 tcgacgcgct gagcgacgtc ccggcgctcg tcgcggtgcg caataaaatc gacgccggcg
     2041 agatggtagg acctcgcctc aagatctcgg gcttagtgcc gctgaaccag acggcgttgc
     2101 cgggcggtgt tgaccccgcg cggcgcgacc ccgcgcgcat tccgttcaac cagcggccgc
     2161 ccgtcgactg cacggcgccc gatcaagcgc gcggcatggt gaaccgcatc gccgatcaag
     2221 gcggggacaa catcaagatc attctcgtgg cgagcccgaa gaattgcgat ctcgcgacgc
     2281 tgcgcgcgat ctccgacgag gcgcacaagc gcgggctccg catgatcacg cacaacacgg
     2341 cggtcgtgga tgccgtcatg gcggccgatg cgaagaccga ctacttcgcg cacacgccgc
     2401 acatcggctg ggtggacgag ggcgacgcga tccggaagat cgtcgcggcg ggcgcgccga
     2461 tggtcacgac gctcggcgtg ttcacgccgt acttcgacga cacgcaccac gacggcctgt
     2521 tccgcgacaa caacgagttc caatgggacg cgctgcacag cgcgggccaa ggccccgtca
     2581 acgatcggtt cctctggaac gccggtgtcg tgatcggcta cggcaccgat acgtcgttcg
     2641 cgccgcgcga ctcgctgcgg cacgagctgc gcgcgctgtc gtccgtgttc gggccgaagg
     2701 agctcgtcac gatcctgacg aagaacgcgg cgatctgcgc cgacgtcgac gagatcacgg
     2761 gcacgatcga gaccggcaag caggccgatc tcgtcatgct cgacggcggc gatccgctga
     2821 aggacatcgt cgcggtgctc gacgtgcggc tcgtcgtcaa aggcggcaag atcgtttccg
     2881 accgccgcta atggggtgcc ggtgttacgg ttcggttgaa ccgaacccga aacaccggca
     2941 cctctttagt gcttcagcgg gttttcctgg ccggcggtga agcggttggg
//
LOCUS       DASSNN010000371         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1079266, whole genome shotgun sequence.
ACCESSION   DASSNN010000371 DASSNN010000000
VERSION     DASSNN010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079266"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VFL84_06780"
     CDS             <1..497
                     /locus_tag="VFL84_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HET7608363.1"
                     /translation="KPVMYIANVAEDSPEVNPLVEQVRKIAAEQGAEVVVISAAIEAE
                     LAQLPVGERAEFLATLGLTEPGLNRVIRSAYKLLGLKTFYTAGPKEVRAWTTKRDATA
                     PQAAGAIHTDFERGFIRAEVIGYDQYIARRGEQGAREAGELRLEGKTYVVQEGDVIHF
                     RFNV"
     gene            517..1776
                     /locus_tag="VFL84_06785"
     CDS             517..1776
                     /locus_tag="VFL84_06785"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HET7608364.1"
                     /translation="MNVMVLGAGLLGVTSAYFLRQQGHDVTVVDRQATPAAETSFANG
                     GQISVSHAEPWANPSAPLKVLKWMTKEDAPLLFRVRADLRQWLWTLAFLRECTPGRTR
                     RNIEHIVRLGTYSREMLQQLRRDIGIDYDQRTQGILHFYTSAKELAAAEAPAALMREL
                     GCDRRLISVDEAVRIEPALRHIRPKLAGATYTAQDESGDANRFARELVEHCQADGVRF
                     LMSHTVTALRAFGGQLDHVEATDYEGRFQFLRADAYVLALGSLSPVYARPLGLRLPIY
                     PAKGYSVTMPVRDPARAFQVSLTDDEYKIVFSRLGDKLRIAGTAELNGYDRDLNRVRC
                     EALVRRTEELFPGAGDPERAQFWTGLRPATPSNVPIVGRSTRLPNLYLNTGHGTLGWT
                     HACGSGKSIARIVSGLKPEVDFPFVGV"
     gene            1845..>2156
                     /locus_tag="VFL84_06790"
     CDS             1845..>2156
                     /locus_tag="VFL84_06790"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7608365.1"
                     /translation="MPDPYASIASADATLQERLAAVLELRAADPQQRAMLDAYLAEVA
                     LPPSAIVLDAGCGTGAVARALSQRPNVREVVGLDPSPIFVETAREFAKGMPGLSFHVG
                     DA"
BASE COUNT          351 a          726 c          740 g          339 t
ORIGIN      
        1 cgaagcccgt catgtacatc gcgaacgtcg ccgaggattc gccggaggtg aacccgctcg
       61 tcgagcaggt gcgcaagatc gcggccgagc aaggcgccga ggtcgtcgtg atctccgcgg
      121 ccatcgaggc cgagctcgcg caattgcccg tcggcgagcg cgcggagttc ctcgcgacgt
      181 tgggcctcac ggagccgggc ttgaaccgcg tgatccgctc ggcctacaag ctgctcggtt
      241 tgaagacgtt ctacactgcc ggccccaaag aggtgcgcgc gtggacgacg aagcgcgacg
      301 cgacggcgcc gcaagccgcg ggcgcgatcc ataccgattt cgagcgcggg ttcattcgcg
      361 ccgaggtcat cggctacgac cagtacatcg cgcggcgcgg cgagcaaggc gcgcgcgagg
      421 ccggggagct gcggctcgag ggcaagacct acgtcgtcca ggaaggcgac gtgattcatt
      481 tcaggttcaa cgtctgaggc gcgaggtgtc gccgccgtga acgtcatggt gttgggtgcg
      541 ggcttgctgg gagtcacctc ggcgtacttc ctgcgccagc aggggcacga cgtcacggtc
      601 gtcgaccgcc aggcgacgcc ggccgccgag acgagcttcg cgaacggcgg ccagatttcc
      661 gtcagccacg cggagccgtg ggcgaacccg agcgcgccgc tcaaagtgct gaagtggatg
      721 acgaaggagg acgcgccgct cctgttccgc gtgcgcgccg acttgcgcca gtggctctgg
      781 acgctcgcgt tcttgcgcga gtgcacaccg ggacgaacgc ggcgcaacat cgagcacatc
      841 gtgcggctcg gcacctacag ccgcgagatg ctgcagcagt tgcgccgcga catcggcatc
      901 gactacgacc agcgcacgca aggcatcctg catttctaca cgagcgcgaa ggagctcgcg
      961 gccgccgagg cgccggccgc tctgatgcgc gagctcggct gcgaccgccg tctgatcagc
     1021 gtcgacgagg ccgtgcgcat cgagcccgcg ctgcgccaca tccggcccaa gctcgcgggc
     1081 gctacctaca cggcgcaaga cgaatccggc gacgcgaacc gcttcgcgcg cgagctcgtc
     1141 gagcactgcc aggccgacgg cgtgcggttc ctgatgagcc ataccgtcac ggcgctgcgc
     1201 gcattcggcg gccagctcga ccacgtcgaa gcgacggact atgaagggcg cttccagttc
     1261 ctgcgcgccg atgcgtacgt gctcgcgctc ggcagcttga gccccgtcta cgcgcggccg
     1321 cttgggctta ggctcccgat ctatcccgcg aaaggctact ccgtgacgat gcccgtgcgc
     1381 gacccggcgc gcgcattcca ggtatcgctg acggatgacg agtacaagat cgtcttctcg
     1441 cggctcggag acaagttgcg aatcgcgggc acggcggagc tcaacggcta cgaccgcgac
     1501 ttgaatcgcg tgcgctgcga ggcgctcgtg cggcgcacgg aggagctctt cccgggcgcc
     1561 ggcgatccgg agcgcgcgca gttctggacg ggcttgcgcc ccgcgacgcc gtcgaacgtg
     1621 ccaatcgtcg gccgctcgac gaggctgccg aatctatact tgaacacggg gcacggcaca
     1681 ctgggctgga cgcacgcgtg cggctccggc aagagcatcg cgcgcatcgt cagcgggttg
     1741 aagcccgagg tggatttccc tttcgtcggc gtttagagag acctgtatcg ttgtgctcgt
     1801 cgggtcgcgc gccgccggtt gtcgaccgcc cacgacggag aagcatgccg gatccctatg
     1861 cttcgattgc ttcggccgat gcgacgctgc aagaacggct cgccgcggtc ctcgagctgc
     1921 gcgcggccga cccgcagcaa cgcgcgatgc tcgacgccta tctggcagag gtagcgcttc
     1981 cgccttcggc aatcgtgctc gacgcggggt gcgggaccgg cgcggtcgcc agggctctct
     2041 cgcaacgccc gaacgttcgc gaagtcgtcg gtctcgaccc ttcgccgata ttcgtcgaga
     2101 cggcccgcga attcgccaaa ggaatgccgg gactctcttt ccatgtcgga gacgct
//
LOCUS       DASSNN010000372         3676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1450900, whole genome shotgun sequence.
ACCESSION   DASSNN010000372 DASSNN010000000
VERSION     DASSNN010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450900"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VFL84_06795"
     CDS             <1..428
                     /locus_tag="VFL84_06795"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HET7608366.1"
                     /translation="RDGAAFGPFNLFVYDGAELWFASNRAAASRLGAGLHSFSNAPAG
                     VEWPKTASARAGVERLLSHPFPIEPLFALLGERDGSGTPSERYQRAHFVVGPVYGTRC
                     STVMLVDSGGQVTFAERSFDAAGARVADVREAFQIERAI"
     gene            complement(425..1084)
                     /locus_tag="VFL84_06800"
     CDS             complement(425..1084)
                     /locus_tag="VFL84_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7608367.1"
                     /translation="MLDAIEITTGPEPRLAVIWLHGLGADGHDFEPIVPELRLGFAVR
                     FVFPHAPVRPVTINGGMAMRAWYDILGFDRRAKEDSAGIRSSAAAVTELVDRELERGM
                     TADRVVLAGFSQGGAIALHTALREPRPLAGVLALSTYLPLAATLANERSAANMRIPLF
                     MAHGTDDGVLPLQLAESSRRTLEGLGYAVDWHVYPMAHSVSIEEVGAIGAWLAALPGA
                     R"
     gene            1154..1618
                     /locus_tag="VFL84_06805"
     CDS             1154..1618
                     /locus_tag="VFL84_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HET7608368.1"
                     /translation="MPSATLEQNERRRAIARLLAEHVVNRQAELVELLRAEGYLATQS
                     SVSRDLRDLGAAKLRDGYSLPAPAEIGDDESLSVVAEFVRDIRSAGPNLLVVITEIGA
                     AQRVAVTLDNSGWPEIVGTVSGDDTIFVATIGVSEQRRLRSRLQQHLKKAPT"
     gene            1615..2823
                     /gene="argG"
                     /locus_tag="VFL84_06810"
     CDS             1615..2823
                     /gene="argG"
                     /locus_tag="VFL84_06810"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET7608369.1"
                     /translation="MTSSSNPIVLAFSGGLDTSFCVPWLKETYKRPVVTVTVNCGGID
                     SAAARALDERARSLGAADHHLIEARPEFFDRVIKYLIMGNVRRGNMYPLSVGAERGLQ
                     AKYLAKLAKELGSDTVAHGCTAAGNDQVRFEIVLKAVAPELTILAPVRDHAFQRPAQV
                     KFLEERKMPVPTRGSAYSTNRGLWGVTIGGSETLDSKGSIPESAWVLSAGALEKPQPF
                     LEIEIEFAQGVPVGLNGERLDAVALIEKVEELGARYGIGRGIHLGDTIIGTKGRVAFE
                     APAAEILISAHRELEKLTLLGSQATVKEPVSQVYGQLIHEGKQLDPACRDIEALLQSS
                     QARVTGKVRVQLRPGSLFVLGVTSPHSLMAVSRGKYGEAAGEWTPADAYGFSKIMALP
                     GMFHTRAGGQ"
     gene            2825..>3676
                     /locus_tag="VFL84_06815"
     CDS             2825..>3676
                     /locus_tag="VFL84_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608370.1"
                     /translation="MRSVLVDKIASVAVQADLGQEIRVSADIPCEEGVLVAVEVLNNK
                     SRYNTIELTSGRMAEVKKGDVVVGALGHRKALFGYSGHLPTQLVPGDTVQLLNLGGVI
                     GICDSINPSHGEPFNCKVLGCVLDFPYLGQRVGVPARVSTEPLKLDVPLDTHGVPIVA
                     IAGTCMNSGKTAAACAIVSRFRHNGLTVDAFKSTGVALRRDILAMEDSGARHIAMFSD
                     FGICATTSKNAPALTRSLLNELSDEKPDAIVFELGDGLLGAYGVEAILRDQEIVKRIT
                     AMVLCAND"
BASE COUNT          612 a         1218 c         1247 g          599 t
ORIGIN      
        1 ttcgcgacgg cgcggcgttc ggcccgttca acttgttcgt ctacgacgga gccgagctct
       61 ggttcgcgag caatcgcgcc gccgcgtcga ggctcggcgc ggggcttcat tcgttcagca
      121 atgcgccggc cggcgtcgag tggccgaaga ccgcgagcgc gcgcgccggc gtcgagcggt
      181 tgctgtcgca cccgttcccg atcgagccgc tgttcgcttt gctcggcgag cgcgacggat
      241 cgggtacgcc ctcggagcgc taccaacgag cgcatttcgt cgtcgggccc gtctacggca
      301 ctcgctgctc tacggtcatg ctcgtcgact cgggcgggca agtgacgttc gcggagcgct
      361 cgttcgatgc ggccggagct cgcgtggccg acgtgcgcga agcgttccag atcgagcgcg
      421 cgatctagcg cgcgcccggt agcgccgcga gccaggcgcc gatcgcgccg acctcttcga
      481 tcgagacgga gtgcgccatc gggtagacgt gccaatcgac ggcgtagccg agcccctcga
      541 gcgtgcgccg cgacgactcg gcgagctgaa gcggcagcac gccgtcgtcc gtgccatgcg
      601 ccatgaagag cggaatgcgc atgttcgcgg cgctgcgctc gttcgcaagc gtcgcggcga
      661 gcggcagata cgtcgagagc gcgagcacgc cggccaacgg ccgcggctca cgcagcgccg
      721 tgtgcagcgc gatcgcgccg ccctgcgaga atcccgcgag cacgactcga tccgcggtca
      781 tgccgcgctc gagctctcga tcgacgagct cggtgacggc ggccgcgctc gagcgaatgc
      841 ccgccgaatc ttctttcgcg cgacggtcga acccgaggat gtcgtaccac gcgcgcatcg
      901 ccatgccgcc gttgatcgtc acgggacgta ccggggcgtg cgggaacacg aaccgcaccg
      961 cgaagccgag ccgaagctcg ggcacgatcg gctcgaagtc gtggccgtcg gctccgaggc
     1021 cgtgcaacca gatcaccgcg aggcgcggct cggggcccgt tgtgatctcg atcgcatcca
     1081 gcatgcatga ccctcttgcg gacttgcgca tatggcctat tatatgcgca actttattca
     1141 cgcctccttt tccatgccga gtgcaaccct cgaacaaaac gagcggcgac gagctattgc
     1201 ccgcctgctc gccgagcacg tggtgaaccg acaagcggag cttgtcgagc tgttgcgcgc
     1261 cgaagggtac ctcgcgactc agtcgagtgt cagccgggat ctgcgcgacc tcggcgccgc
     1321 gaagctcagg gacggctaca gcttgcccgc accggcagag atcggcgacg acgagtcgtt
     1381 gtcggtcgtc gcggaattcg tgcgcgacat ccgctcggcc gggccaaacc tgctcgtcgt
     1441 catcaccgag atcggagctg cgcagcgcgt cgccgtcacg ctcgacaaca gcggctggcc
     1501 cgagatcgtc ggcacggtct ccggcgacga cacgatcttc gtcgcgacga taggcgtaag
     1561 cgaacaacgc cggcttcgca gccggctgca acaacatctg aagaaggcac cgacatgacc
     1621 tcaagctcaa atccaatcgt gctcgcgttc tcggggggcc tcgacacgtc gttttgcgtg
     1681 ccgtggctca aggagacgta caagcgcccg gtcgtgacgg tgaccgtcaa ttgcggcggc
     1741 atcgactccg ccgctgcacg cgcgctcgac gagcgcgcgc gctctctcgg cgcggccgac
     1801 catcacctca tcgaagctcg gccggagttc ttcgatcgcg tcatcaagta cttgatcatg
     1861 ggcaacgtgc gccgcggcaa catgtacccg ctgtcggtcg gcgccgagcg cggcctgcaa
     1921 gcgaagtacc tcgcgaagct cgcgaaagag ctcggctccg acaccgttgc gcacggctgc
     1981 accgcggccg gcaacgacca ggtgcggttc gagatcgtgc tgaaggccgt cgcgcccgag
     2041 ctcacgatct tggcccccgt gcgcgaccac gcgttccagc ggcccgctca agtgaaattt
     2101 ctcgaggagc gcaagatgcc ggtgccgacg cgcggctcgg cgtactcgac gaaccggggc
     2161 ttgtggggcg tcacgatcgg cggcagcgag acgctcgatt cgaagggcag cattccggaa
     2221 agcgcatggg tgctctcggc cggcgcgctc gagaagccgc agccgtttct cgagatcgag
     2281 atcgagttcg cgcaaggcgt gcccgtcggc ttgaacgggg agcggctcga cgccgtggcc
     2341 ctgatcgaga aggtcgaaga gctcggcgcg cgctacggta ttggccgcgg cattcacttg
     2401 ggcgatacga tcatcggcac gaaaggccgc gtcgcattcg aggcgccggc ggccgagatc
     2461 ctgatcagcg cgcaccgcga gctcgagaag ctgacgctgc tcggctcgca ggcgacggtc
     2521 aaagagccgg tgagccaggt ctatggtcag ctgatccacg agggcaagca gctcgacccc
     2581 gcgtgccgcg acatcgaggc gctgcttcag tcgtcgcagg cgcgcgtgac cggcaaggtc
     2641 cgcgtgcagc tgcggcccgg cagcttgttc gtgctcggcg tcacgtcgcc gcattcgttg
     2701 atggccgtga gccgcggcaa atacggcgag gccgccggcg agtggacgcc ggccgacgcc
     2761 tacggtttct cgaagatcat ggctctgccc ggcatgttcc acacccgggc cggaggacag
     2821 tgagatgcgc tcggtactcg tcgacaagat cgcttcggta gccgttcaag cggatctcgg
     2881 ccaagagatt cgcgtgagcg cggacattcc gtgcgaggaa ggcgtgctcg tcgccgtaga
     2941 ggtgctcaac aacaagtcgc gctacaacac gatcgagctc acgagcggcc gcatggccga
     3001 ggtgaaaaag ggcgacgtcg tcgtcggcgc gctcgggcac cgcaaggcgt tgttcggcta
     3061 ctcgggccat ttgccgacgc agctcgtgcc cggcgacacg gtgcagctgc tgaacttagg
     3121 aggcgtcatc ggcatctgcg actcgatcaa tccgtcgcat ggcgagccgt tcaactgcaa
     3181 ggtgttgggc tgcgtgctcg acttcccgta tttgggccag cgcgtcgggg tgccggcgcg
     3241 cgtttcgacc gagccgctga agctcgacgt gccgctcgac acgcatggcg tgcccatcgt
     3301 cgcgatcgcg ggcacgtgca tgaactccgg caagacggcc gcggcgtgcg cgatcgtcag
     3361 ccgctttcgc cataacggct tgacggtcga tgcgttcaag tcgaccggtg tcgcgctgcg
     3421 ccgcgacatc ttggccatgg aggactcggg cgcgcggcac atcgcgatgt tctccgactt
     3481 cggcatctgt gcgacgacgt cgaagaatgc gcccgcgctc acgcgctcgc tgctgaacga
     3541 gctgtcggac gagaaacccg acgccattgt cttcgagctc ggcgacgggc tgctcggcgc
     3601 gtacggcgtc gaggcgatct tgcgcgacca ggagatcgtc aaacgaatca cggcgatggt
     3661 gctctgcgcg aacgac
//
LOCUS       DASSNN010000373         4377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_671322, whole genome shotgun sequence.
ACCESSION   DASSNN010000373 DASSNN010000000
VERSION     DASSNN010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671322"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VFL84_06820"
     CDS             complement(<1..809)
                     /locus_tag="VFL84_06820"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET7608371.1"
                     /translation="MFEQALFLAAGLATGAAVGFAIAWAKGSRAREELAALDARAEEQ
                     EKAAADKLAHVTNMRRELADAFKALSAQALDSTSATFLKLAKAELGQFQEKAQSDLKE
                     RQNAVDALVQPIKESLTKVDGKIEQLERSRLNAYSSLNEQLRSLVETHLPQLRSETAN
                     LVKALRQPTVRGRWGELQLKRVVELAGMLEHCDFVEQPTADGEDGRQRPDVIVKLPGG
                     RQIVIDAKVPIAAYLDAHEAADDLERNQHLERHAELVRTHAAALGRKAYWDT"
     gene            complement(945..1196)
                     /locus_tag="VFL84_06825"
     CDS             complement(945..1196)
                     /locus_tag="VFL84_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608372.1"
                     /translation="MMKVTADQRVDPGSDDPVELATLRYRAAFAVYEFVVDKNARMSM
                     RGDKPSKRARLDEERAYEELDSARHALFTAAAVAFPTIH"
     gene            complement(1193..1975)
                     /locus_tag="VFL84_06830"
     CDS             complement(1193..1975)
                     /locus_tag="VFL84_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608373.1"
                     /translation="MSTQLHVSTMVVVAIVSAFGLAPRAEAQGATGVAGAAAVPDNAT
                     ARRYGGSWSCNRGYRRVGDTCVAVVIPPNAYLSTFGDDWECERLFREQDGACKAVEIP
                     ANAYADERSFGPGWRCEQGYRESAGACVAVQVPANAFAVDSTFGTGWRCESRFRESGG
                     ACEPVVVPANAVLDSSGQRWSCKRGYVLDGQRCTAVEVPANAYLNAAGSGWRCERGFG
                     SEGASCIALKVPSNAHLDYSGNDWVCDENHRRTADSCTVDPP"
     gene            2172..2513
                     /locus_tag="VFL84_06835"
     CDS             2172..2513
                     /locus_tag="VFL84_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608374.1"
                     /translation="MILGKAHARNRRSTPAVDPNLEEAYKLVGIEAVSTPEGCTGRDW
                     HVYRIAQGANVITGYRQGSLVAATSEVEKIVVGLNERRVASKGRPGPKPKGSTDAAKA
                     AAPPDADDGAS"
     gene            complement(2574..3284)
                     /locus_tag="VFL84_06840"
     CDS             complement(2574..3284)
                     /locus_tag="VFL84_06840"
                     /inference="COORDINATES: protein motif:HMM:NF016883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytochelatin synthase family protein"
                     /protein_id="HET7608375.1"
                     /translation="MSTCTESRCPPAATAAPPERTLYMRPLPATLAALDSAAGRAAFQ
                     RALLAGTLEGYFKIAQQFHTQADPAFCGLGSLVSALNALGIDPKRAWKGSWRWFSEEL
                     LDCCKSLDEVRRAGITLDEAACLARCSNAEVTVRRAGEASAGEFREHLLAAATASDGP
                     VLIANYSRSAVGQTGTGHFSPIAGFDAATDQALVLDTARFKYPPHWMPVELLFAAMQE
                     RDADTGDTRGWLLLRRAA"
     gene            3443..>4377
                     /locus_tag="VFL84_06845"
     CDS             3443..>4377
                     /locus_tag="VFL84_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7608376.1"
                     /translation="MEASTTPALEHARRHHAARAWTLAFEAFQAADRSQELDADDLQR
                     FATAAYLVGRDDDYLALLERAHDLHLRQGRPLQGVRCAFWLGLRLLFRGEGGRASGWF
                     SRAERLVESHAAACAERAYLLFPAVDQAIRSGELEKAASMAAAMVEMGERFADAELVT
                     CSRFDEGLILLRQGDVARGLARLDEVMLAVTSDKLWPIVTGLMYCAVIGACQEVCAAS
                     RAREWTDALSAWCGQQPEMMAFTAACLVHRAEVMRLNGAWGDALAEAERASRRDPQRD
                     PRSTADAFYEMAEIHRLRGDHREAEAAYRDASRYGR"
BASE COUNT          641 a         1514 c         1516 g          706 t
ORIGIN      
        1 gtatcccagt aggctttgcg gccgagcgcc gcggcgtgcg tgcgcacgag ctcggcgtgg
       61 cgctcgagat gttggttgcg ctcgaggtcg tcggcggctt cgtgcgcgtc gagataggcc
      121 gcgatcggca ctttcgcatc gatgacgatc tgccggccgc cgggcagctt gacgatgacg
      181 tcggggcgct ggcgtccgtc ctcgccgtcg gccgtcggct gctcgacgaa atcgcaatgc
      241 tcgagcatgc ccgcgagctc gacgacgcgc ttcagctgca gctcgcccca gcggcccctg
      301 acggtcggct ggcgcagcgc cttcacgaga ttcgcggtct cgctgcgcag ctgcggcagg
      361 tgcgtctcga cgaggctgcg cagctgctcg ttcagcgacg agtacgcgtt caagcgcgag
      421 cgctcgagct gctcgatctt gccgtcgact ttcgtcagcg attctttgat cggctgcacg
      481 agcgcgtcga cggcgttttg ccgttctttg agatcgctct gcgccttttc ttggaactgg
      541 ccgagctcgg ctttcgcgag cttcaggaac gtggcgctcg tgctgtcgag cgcttgcgcc
      601 gacagcgcct tgaacgcatc cgcgagctcg cggcgcatgt tcgtgacgtg tgcgagcttg
      661 tcggcggcgg ctttttcttg ctcctccgcg cgtgcgtcga gcgcggcgag ctcctcgcgc
      721 gccctgctgc ctttggccca ggcgatggcg aagccgacgg ccgcgccggt ggcaaggccg
      781 gcggccaaga acagtgcttg ctcgaacatg gcaggcgccc ctctttgaat cgttgacgga
      841 gggcattcga gcaggcgccg gtcgcaaaac gtgacaccgc tgctccggcg cgctaacgcg
      901 cgcggggccc gagccgccgc cgcacccgcc acgcaggccg ccagtcagtg aatcgtcgga
      961 aaagccaccg ccgcggccgt gaacaacgca tggcgagcgg agtcgagctc ttcgtacgct
     1021 cgctcttcat cgagccgcgc tcgcttcgag ggtttgtcgc cgcgcatgct catccgggca
     1081 ttcttgtcga cgacgaattc atacaccgcg aaggcggccc tgtagcgcaa cgtggcgagc
     1141 tcgacggggt cgtcgctgcc cggatccacg cgttgatcgg cggtgacctt catcacggcg
     1201 gatccacggt gcagctgtcc gcggttcttc gatggttctc gtcgcaaacc cagtcgttgc
     1261 ccgagtaatc gaggtgcgca ttgctcggca ctttcagcgc gatgcagctc gcgccttcgc
     1321 tgccgaaccc gcgctcgcaa cgccaaccgc tgcccgcggc attcagatac gcgttcgcgg
     1381 ggacctcgac ggcggtgcac cgctgcccgt cgagcacata gccgcgcttg cacgaccagc
     1441 gctggcccga gctgtcgagc acggcgttcg ccggtaccac gacgggttcg caggcgccgc
     1501 cgctctcgcg aaagcggctc tcgcatcgcc agccggtgcc gaaagtcgaa tcgacggcga
     1561 acgcgttcgc gggaacctgc acggcgacgc acgcgcctgc gctctctcga tagccctgct
     1621 cgcaacgcca gccgggcccg aacgagcgtt cgtcggcgta ggcgttcgcg ggaatctcca
     1681 cggccttgca ggcgccgtct tgctcgcgaa acaaccgctc gcactcccaa tcgtcgccga
     1741 aggtgctcaa gtatgcgttc ggcggaatca cgaccgcgac gcacgtgtcg ccgacgcgcc
     1801 gatagccgcg attgcagctc cagctgccgc cgtagcggcg cgccgtcgcg ttgtccggca
     1861 ccgcggccgc gccggccacg ccggttgcgc cctgcgcctc tgcgcgcggc gcgaggccga
     1921 atgcggacac gattgcgacg acgaccatcg tgctcacgtg gagctgcgtg ctcatgccgt
     1981 gtgtccttct gctgggggaa gcgccggcga gatccggcta ccagcagtct actcgcaatc
     2041 gctcgcgcga ttcgcgctat ttggccgggg catcctgtac actggttgcc agccggattc
     2101 ggccggcgtc tcccgcgaag gaacacgcgg atgcgcattc gactgttcac gcgcgccgcc
     2161 acgcttacac agtgatcctc ggcaaagctc atgctcgcaa tcgaaggtcg acaccggcgg
     2221 tcgaccccaa cctcgaggaa gcgtacaagc tcgtcgggat cgaagccgtg agcacgccgg
     2281 agggctgcac gggccgcgat tggcacgtct accggatcgc gcagggcgcg aacgtgatca
     2341 cgggctatcg ccagggcagc ttggtcgccg cgacgagcga ggtcgagaag atcgtcgtcg
     2401 gtttgaacga gcggcgcgtc gcgagcaagg ggcggcccgg tccgaagccg aaggggtcga
     2461 cggacgcggc taaagcggcc gcaccgccgg atgccgacga cggcgcgagc tgagccgtct
     2521 taatccgcga agcactcgaa cgcttacctc gcgatgacct cgccgcgcaa tcgtcacgcg
     2581 gcgcggcgca acagcagcca accgcgcgtg tcgccggtgt cggcgtcgcg ctcttgcatc
     2641 gccgcgaaca acagctcgac cggcatccaa tgcggcggat atttgaagcg cgccgtgtcg
     2701 agcaccaatg cctggtccgt cgcggcgtcg aagcccgcga tcggagagaa gtggccggtg
     2761 ccggtctggc cgacggcgct gcgcgaatag ttcgcgatca gcaccgggcc gtcgctggcg
     2821 gtggccgccg cgagcaggtg ctcgcgaaac tcgccggcgc tcgcctcacc ggcgcggcgc
     2881 acggtgactt cggcattgct gcagcgcgcg aggcaggccg cttcgtcgag cgtgatgccg
     2941 gcgcggcgca cctcgtcgag cgatttgcag cagtcgagca gctcctcgct gaaccagcgc
     3001 cacgagccct tccaggcgcg cttgggatcg atgccgagcg cgttcagcgc actgacgagc
     3061 gagcccaagc cgcagaacgc gggatcggcc tgcgtgtgga actgctgcgc gatcttgaaa
     3121 tagccctcga gcgttcccgc gagtaacgcg cgctggaacg cggcgcggcc ggccgcggaa
     3181 tcgagtgccg cgagcgtggc gggcagcggc cgcatgtaca gcgtgcgctc ggggggagcc
     3241 gccgtggcgg cgggcggaca tcgggactct gtgcaggtgc tcatggggct gatccttccg
     3301 gctgcctgtg cctcgaaatg aggcaagaac gccgccgtgc aaggggcttg aacgtagcgg
     3361 ctattctcgg gccggtcttg cgccacgcgc aacgccgcga tttgcattac cctccctcgg
     3421 aacgaacgga gaggacccgc cgatggaggc ctccaccacc cccgctctcg agcacgctcg
     3481 ccgccaccac gccgcgcgcg cgtggacgct cgcgttcgaa gcgttccaag cggcggaccg
     3541 ctcgcaagag ctcgacgccg acgatctcca gcgcttcgcg acggccgcat atctcgtcgg
     3601 ccgcgacgac gactatctcg cgctgctcga gcgcgctcac gacctgcatc tgcgccaagg
     3661 ccggcctctg caaggcgtgc gctgcgcgtt ctggctcggc ttgcggctct tgttccgcgg
     3721 cgagggcggc cgggcgagcg gctggttttc gcgcgccgag cggctcgtgg agtcgcacgc
     3781 cgccgcctgc gccgagcgcg cctacctgct gttccccgca gtggaccagg cgattcgcag
     3841 cggcgagctc gagaaggccg cttcgatggc ggccgcgatg gtggagatgg gcgagcgctt
     3901 cgcggacgcc gagctcgtca cttgctcgcg tttcgacgag gggctgatcc tgctgcggca
     3961 gggcgacgtc gcgcgcggcc tcgcgcggct cgacgaggtc atgctcgccg tcacctcgga
     4021 caagctctgg ccgatcgtca cgggcctcat gtactgcgcc gtcatcggcg cgtgccagga
     4081 agtctgcgcg gcgagccgcg cgcgcgagtg gaccgacgcg ctttccgcct ggtgcgggca
     4141 gcagccggag atgatggcgt tcacggcggc gtgcctcgtg catcgcgcgg aagtcatgcg
     4201 cttgaacggc gcgtggggcg acgcgctcgc ggaagccgag cgcgcgtcgc ggcgcgatcc
     4261 gcagcgcgac ccgcgctcca ccgccgacgc gttctacgag atggcggaga tccatcgcct
     4321 gcgcggtgat caccgcgagg cggaggccgc ctaccgcgac gcgagccgct acggccg
//
LOCUS       DASSNN010000374         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_444903, whole genome shotgun sequence.
ACCESSION   DASSNN010000374 DASSNN010000000
VERSION     DASSNN010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444903"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..319
                     /locus_tag="VFL84_06850"
     CDS             50..319
                     /locus_tag="VFL84_06850"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HET7608377.1"
                     /translation="MDRDRGSERQSRRWVEADGIHIDVRGLPPPQPMVSILTLVESIR
                     DATPVVVHHERDPLFLYPELAEIGWTSERLDAPAGEVRLLLTRAA"
     gene            337..1770
                     /locus_tag="VFL84_06855"
     CDS             337..1770
                     /locus_tag="VFL84_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608378.1"
                     /translation="MTGAAREPVPEKAKAASFAPLDAADASEAPRSAKPGQDPRGRKV
                     KTTPGSFLGGAIGRLLPASIPFRYFGAAAVFHVAAWVALFAGADGAPRYRGGLGWTLA
                     SLHLVTLGVLTMTAIGASLQLLPVATRQAVHSRHAPALVFWLYAPGVAAAAIGMGWPS
                     VPLLATGALLIALALAIYAALLARNLFGARGMPAVIAHVWVAWIALAIVLVAALSLAF
                     GYAGGSALPRAVALALHVPFAAYGFMGMLALGLSYILVPMFALSQPPDERTALVSCAL
                     ACAALAAVAAAAFGIAVVPLRVAAIGAGGVAVALHLRLMLRSLANGMRRDLGPSFRLV
                     RLGWGMLAASLAAALGVALDVPFDGMATLFGLLLIAGWLLTFLLGILQRIVPFLASMH
                     ASRGRHRPPTPSSLAAGPALRVHAMCHVSALAVLAAAIVIDNAWLARIGALLGLVGSV
                     AFLAFFVQVVRRMRGAGAEGRARAASA"
     gene            1860..>2451
                     /locus_tag="VFL84_06860"
     CDS             1860..>2451
                     /locus_tag="VFL84_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET7608379.1"
                     /translation="MSYQRQVSRTLDDEHRVNLELLGRIEQAFARAPRSGNAGNAELT
                     QLAGVLARHIEQDVGRHFDFEEQELFPRMSDAGDGDMASLLVEEHDAIRACASEVLPL
                     AQAAAAGTLDDAGWTTLKRGALEMVERQVAHIQKETMALLPLLEDLLDASEQLEIDAM
                     LVVERTTHLALVGHRRISLMRTTVAPAGRCAIDRRQA"
BASE COUNT          301 a          847 c          870 g          433 t
ORIGIN      
        1 gctgatcatc ggcacgcgcg cactggagcc gcggcccgct tagccgatca tggatcgcga
       61 tcgaggcagc gaacgccaat cccggcgctg ggtggaggcc gacggcatcc acatcgacgt
      121 gcgcgggttg ccgcccccgc agccgatggt gtcgatcctg acgctcgtcg agtcgattcg
      181 cgatgcgacg ccggtggtcg tgcaccacga gcgcgacccg ctgttcctgt atcccgagct
      241 cgcggagatc ggctggacgt cggagcgcct cgacgcgccc gccggcgaag tgcgccttct
      301 tctcacgcgc gcagcgtgag cggagcgacg atgcacatga ccggcgctgc ccgcgaaccg
      361 gttccggaaa aagcgaaagc ggcgagcttc gccccgctcg atgcggccga tgcgagcgaa
      421 gcgccacgct ccgcgaagcc cgggcaggat ccgcgcggcc gcaaggtgaa gacgacgccc
      481 ggctcgtttc tcggcggcgc gatcggacgg ctgttgccgg cgtcgattcc gtttcggtac
      541 ttcggcgccg ccgccgtatt tcacgtcgcc gcttgggtcg cgctcttcgc gggcgccgac
      601 ggcgcgccgc gctatcgagg cggcttaggc tggacgctcg cctcgctgca cctggtgacg
      661 ctcggcgtgc tcacgatgac ggcgatcggc gcgagcctcc agcttctccc cgtcgcaacg
      721 cggcaggccg ttcactcgcg gcatgcgccg gcgctcgtat tctggctgta tgcgccgggc
      781 gtcgcggccg cggccatcgg catgggatgg ccttcggtgc cgctgctggc gaccggtgcg
      841 ctgttgattg cacttgcgct cgcaatctat gcggcgctgc tcgcacgcaa tctgttcggc
      901 gcgcgcggca tgccggccgt catcgcgcac gtctgggtcg cgtggatcgc gctcgcgatc
      961 gtgctggtcg ccgcgctgtc gctcgcgttc gggtacgcag gcgggtccgc gcttccgcgc
     1021 gcggtcgcac tcgcgcttca cgtgccgttc gcggcctacg gcttcatggg catgctcgcg
     1081 ctgggtcttt cgtacattct cgtgccgatg ttcgcgcttt cgcagccgcc cgacgagcgt
     1141 acggcgctcg tgtcgtgcgc gctggcgtgc gcggcgctcg cggcggttgc cgcggcggct
     1201 ttcggcatcg cggtggtccc gctgcgcgtg gccgcaatcg gcgccggcgg ggtcgcggtc
     1261 gcgttgcatc ttcggctgat gctgcggtcg ctcgccaacg gcatgcggcg agacctgggc
     1321 ccgtcgtttc gcctggtgcg cctcggctgg gggatgctcg ccgcgagcct cgccgcggcg
     1381 ctcggcgtcg cgctcgacgt gccgttcgac ggcatggcga cgctcttcgg cctgctgctc
     1441 atcgccggat ggctgctcac gttcctgctc ggcatcctgc agcgcatcgt gccgtttctt
     1501 gcgtcgatgc acgcgtcgcg cggcaggcat cggccgccga caccttcgtc actcgcggcg
     1561 ggtccggcgc tgcgcgtcca cgccatgtgc cacgtgtcgg cgctcgccgt tctcgccgct
     1621 gcgatcgtca tcgacaatgc gtggctcgcg cgaatcggcg cgttgcttgg cctcgtcggc
     1681 agcgtggcct ttctcgcatt tttcgtgcag gtcgtccggc gcatgcgcgg tgccggcgcc
     1741 gaaggcaggg cgcgcgccgc atcggcctga cggcctcggc agtgccggct tgacgtcgat
     1801 caatggcgcg ctggcatgcg ggtgctaccg tgctcctgcc gcgaatggaa agttccgaca
     1861 tgagctatca gcgccaggtc agccgcacgc tcgacgacga gcaccgcgtc aatctcgaac
     1921 tgctcggacg tatcgagcag gcgttcgcgc gtgcgcctcg atcgggcaac gcaggcaacg
     1981 ccgagctgac gcagcttgcc ggtgtgctcg cgcggcacat cgagcaggac gtcggccggc
     2041 atttcgactt cgaggagcag gagctctttc cgcggatgag cgacgccggc gacggcgaca
     2101 tggcgtcgct gctcgtcgag gagcacgatg ccatccgcgc ctgcgcgagc gaggtgttgc
     2161 cgctcgcgca ggcggccgcc gccggcacgc tcgacgacgc aggctggacg acgttgaagc
     2221 gcggcgcgct cgaaatggtc gagcggcagg tggcgcacat ccagaaggaa acgatggcgt
     2281 tgctgccgct gctcgaggac ctgctcgacg cgtccgagca gctcgagatt gacgcgatgc
     2341 tcgtcgtcga gcgtacgact cacctggcgc tggtaggtca tcgcaggatt tccctcatgc
     2401 gaacgacggt agcaccggcg ggccgatgcg ccattgatcg acgtcaagcg g
//
LOCUS       DASSNN010000375         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_808941, whole genome shotgun sequence.
ACCESSION   DASSNN010000375 DASSNN010000000
VERSION     DASSNN010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808941"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="VFL84_06865"
     CDS             <1..1385
                     /locus_tag="VFL84_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7608380.1"
                     /translation="PMKEAGWVLQQMDKKLAAFPEVASVFGKLGRADTATDAAPVSMI
                     EATIQLKDRKDWRPNLTKDELVAEMDAAMQVVGYANSWVQPINARTIMQDTGIQTPTG
                     IKVKGPDVATIESIGEQIEQLLRSYPGTQSVIAERISSGYFIDVELDPQRLAAAGIST
                     QEAMPVVRFGIGGDNLLAIKDESGALIPLNLQYSPEYLDTLAKVRTSPVVAPDGKTVT
                     LGEIADVAVKKMPDMLRNDNGTLAGYVYVYLNDDVTATDYVAGAQAFLAENLALPAGY
                     AIEWTGDYQNALAAAKRLAIVIPTTLVVIFVLLVLAFRSIADSLLIMLSVPFALTGGV
                     FLQWLLGYPMTTAVIVGYIALFAVAIQTGIVMIIFIRRALARRAENETYMEAVLDGSV
                     LRLRPKLMTVAVTVLSLLPVMLSSGLGMEIMKPIATPTIGGMLTSTIYVLFLIPCLFA
                     IGEDIRRRRA"
     gene            1382..1771
                     /locus_tag="VFL84_06870"
     CDS             1382..1771
                     /locus_tag="VFL84_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608381.1"
                     /translation="MKLASALLCVWLLAAASAAAQGAEPDPESAFAPAQTPDPGSLLP
                     AGPVKPLVIAFCDKCHGLAWIERSGGSLDGWTSRLYRMNRAGAMVPVEQIPVIAEYLA
                     QALPERPRPPEPPKKRSGPSFSSSRGD"
     gene            1774..2190
                     /locus_tag="VFL84_06875"
     CDS             1774..2190
                     /locus_tag="VFL84_06875"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HET7608382.1"
                     /translation="MSHSIRQSAKTLAMLAVTLLGTACGQAPSATAADAGNGLTISFR
                     TLDEPAKGANEIEAVVEQDGKPVTDATVAVTFRMPAMPNMNMPEMHSTVPLTHERDGR
                     YVGTGQLEMAGSWNVTVAVSRNGAQVGSARFSVQAK"
     gene            2208..2618
                     /locus_tag="VFL84_06880"
     CDS             2208..2618
                     /locus_tag="VFL84_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608383.1"
                     /translation="MKALTWWLRIVGTLYLLEGIGLTAMALVDPDRFAATWAVADVGS
                     IDALGVRAALIAGLPGVMTWVLLGAMLWIYSRAPARSRVLVIVVAAWELLVWIPVDVV
                     STLQVFDGGRIVLSAIHAVIGVTGILVLRRLPTE"
     gene            2695..>3222
                     /locus_tag="VFL84_06885"
     CDS             2695..>3222
                     /locus_tag="VFL84_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7608384.1"
                     /translation="MRLPIALPLVFVLGPSALAQAPDGAAVYDRACAACHTNPAPDSR
                     AMPRDALGRLAPETILTALTSGNMFRQGSALSDGERRAVAAFVAGRPVGAPTPRASVG
                     QCESSPRALTASDLAGGWNGWGADARNLRYMPAARAGLTAATVPKLKLKWAFGFAGAS
                     SARAQPAVLGGRVFVA"
BASE COUNT          499 a         1064 c         1126 g          533 t
ORIGIN      
        1 tgccgatgaa agaggcgggc tgggtgctgc agcagatgga caagaagctc gccgcgtttc
       61 ccgaggtcgc gagcgtgttc ggcaagctcg gccgcgcgga caccgcgacg gatgcagcgc
      121 cggtctcgat gatcgaggcg acgatccagc tcaaggaccg caaggactgg cggccgaatc
      181 tgacgaagga cgagctcgtg gccgagatgg acgcggccat gcaggtcgtc ggctacgcga
      241 actcgtgggt gcagccgatc aacgcgcgca cgatcatgca agacaccggc atccagacgc
      301 cgaccggcat caaggtgaag gggcccgacg tcgcgacgat cgagagcatc ggcgagcaga
      361 tcgagcagct gctgcgttct taccccggca cgcaatccgt gatcgcggag cgcatctcgt
      421 cgggctactt catcgacgtc gagctcgacc cgcagcggct cgccgccgcg ggcatctcga
      481 cgcaggaggc catgcccgtc gtgcgcttcg gcatcggcgg cgacaatctc cttgcgatca
      541 aggacgagag cggcgcgctg attccgctga acctgcagta ctcgcccgag tacctcgaca
      601 cgctcgcgaa ggttcgcacc tcgccggtcg tcgcaccgga cggcaagacc gtcacgctcg
      661 gcgagatcgc ggacgtcgcg gtcaagaaga tgcccgacat gctgcgcaac gacaacggca
      721 cgcttgcggg ttacgtgtac gtctatttga acgacgacgt cacggcgacg gactatgtcg
      781 cgggcgcgca agcattcctc gccgagaacc tcgcgctgcc ggcgggctat gcgatcgaat
      841 ggaccggcga ttaccagaac gcgctcgccg cggccaagcg gctcgcgatc gtgattccga
      901 cgacgctcgt cgtgatcttc gtgttgctcg tgctcgcgtt ccgctcgatc gcggacagct
      961 tgctgatcat gctgtccgtg ccgttcgccc tgaccggcgg cgtgttcttg cagtggctgc
     1021 tcgggtatcc gatgacgacg gccgtcatcg tcggctacat cgcgctgttc gcggtcgcga
     1081 tccagaccgg catcgtcatg atcattttca tccggcgagc gctcgcgcgc cgggccgaga
     1141 acgagactta catggaagcg gtgctcgacg gctcggtgct gcggctgcgg ccgaagctca
     1201 tgaccgtggc cgtcacggtg ctctcgctgc tgcccgtgat gctctcgagc gggcttggca
     1261 tggaaatcat gaagccgatc gcgacgccga cgatcggcgg gatgctgacc tcgacgatct
     1321 acgtgctgtt cctgattccg tgcctgttcg cgatcggcga agacatccgg cggagacgcg
     1381 cgtgaagctc gcgtcggcgt tgctttgcgt ttggctcctc gcggcggcga gcgcggccgc
     1441 gcagggggcg gagccggatc ccgagtccgc attcgcgccc gcgcagacgc ccgatccggg
     1501 ctcgctgctg ccggccgggc ccgtgaagcc gctggttatc gcgttctgcg acaagtgcca
     1561 cgggctcgcg tggatcgagc ggtccggcgg ctcgctcgac ggctggactt cgcgcctcta
     1621 ccggatgaac cgcgcgggcg cgatggtgcc ggtcgagcag attccggtca tcgcggagta
     1681 cctcgcgcaa gcgcttcccg aacggccgcg gccgcccgag ccgccgaaga agcgctcggg
     1741 cccttcattt tcgagctcga gaggagacta accatgagcc attcgatccg tcagtcagcg
     1801 aagacgctgg cgatgttggc cgtgacgttg ctcggaacgg cgtgcggcca ggcgccgtcg
     1861 gccactgcgg ccgacgccgg caacggcctt acgatcagct tcaggacgct cgacgagccc
     1921 gcgaagggcg cgaacgagat tgaagccgtc gtcgagcaag acggcaaacc cgtgaccgac
     1981 gccaccgtcg cggtgacgtt tcgcatgccc gcgatgccga acatgaacat gccggagatg
     2041 cacagcacgg tgccgctcac gcacgagcgc gatggccgct acgtcggcac ggggcagctc
     2101 gagatggcgg gcagctggaa cgtgaccgtc gccgtctcgc gcaacggcgc acaggtcggc
     2161 agcgcgcgct tcagcgtcca agcgaagtag ccaagcgtgc gattctcatg aaagcgctaa
     2221 cgtggtggtt acgaatcgtc ggtaccctct acctgctcga gggcatcggc ctcacggcga
     2281 tggctcttgt cgatcccgat cgattcgcgg cgacgtgggc ggttgccgat gtcggctcga
     2341 tcgatgcgct cggcgtccgg gcggcgctga tagccgggct ccccggcgtc atgacctggg
     2401 tgctgctcgg cgcgatgctg tggatttatt cgcgcgcgcc ggcgcgctcg cgagtgctcg
     2461 tgatcgtcgt cgcagcgtgg gagctgctcg tctggatacc ggtcgacgtc gtgtccacgt
     2521 tgcaggtgtt cgacggcggc cgcatcgtgc tcagcgcgat ccacgccgtc ataggcgtca
     2581 cgggaattct cgtgctgcgt cggctaccga ccgagtgacg tcggacgttc gaaattcgcc
     2641 gggtgctgag aggcgtggga tgatgcacgc ctagcgtcga agagcggggg gctcatgaga
     2701 ctgccgattg cgttgccgtt ggttttcgtg ctcggtccgt ccgcgctcgc acaggcgccc
     2761 gacggcgccg ccgtctacga ccgcgcgtgc gccgcttgtc acacgaaccc cgcgcccgac
     2821 tcgcgcgcga tgccgcgcga tgcgctcggc cggctcgcgc ccgagacgat cctcacggcg
     2881 ttgacctcgg gcaacatgtt ccgccagggc tcggcgctgt ccgacggcga gcgccgcgcg
     2941 gtcgctgcgt tcgtcgcggg ccggcccgtc ggcgcaccga ctccgcgagc gagcgtgggg
     3001 cagtgcgaat cttcgccgcg cgcgctgacg gcgagcgacc tcgcaggcgg ctggaacggg
     3061 tggggcgcag acgcgcgcaa cctgcgctac atgccggccg cgagagccgg gctcaccgcg
     3121 gcgacggtgc cgaaactcaa gctcaagtgg gcgttcggct tcgccggcgc gagctccgcg
     3181 cgcgcgcagc cggccgtgct cggcggacgc gtgttcgtgg cg
//
LOCUS       DASSNN010000376        10395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_930065, whole genome shotgun sequence.
ACCESSION   DASSNN010000376 DASSNN010000000
VERSION     DASSNN010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10395
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930065"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFL84_06890"
     CDS             complement(<1..361)
                     /locus_tag="VFL84_06890"
                     /inference="COORDINATES: protein motif:HMM:NF023358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 family protein"
                     /protein_id="HET7608385.1"
                     /translation="MTRTSNNKWSIACGLLALAVSGVAVAQESAAERYARALADAQIT
                     ARYNTQIEQQLRSQEREIASLEEQIANMDATAVDIQSLLQRMYDELVQFVQNDVPFFK
                     EERDQRIERLTELMQTVE"
     gene            complement(384..1697)
                     /locus_tag="VFL84_06895"
     CDS             complement(384..1697)
                     /locus_tag="VFL84_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7608386.1"
                     /translation="MEQQANGSLRLVMTGIALAVSFALAGTALAQGGVPQGQNASNQG
                     TSRAAARNQEEEAPAAGANSIDAQTGKVLNEAIELLNMENYQGAAARINTLNLDRLSP
                     YERGTVERIMFSISYAQEKYEEARGHIQKAIDSGGLNAQQIDELRYQRAQLYMQEEKW
                     KEGAAALEEWFKTAANPNSAAYYLLAVAYYQQEDFNRALAPSKKAVELMDPAKPNESW
                     LSMLSALYLQREEYKEAVPVLQQLIAAAPSKKTYWMQLSSIYGQMEDYPNALAIMQLA
                     HNVGLVTEDSEVRRLADLLLFNDVPYRGAQVLEAAIEKNLVKLDDKLYEKLANCWIAA
                     GEFDKSVGPLQRAAEMAPNGDTFIRLGEVQVQREDWDAAIAAIQRGVEKGQLKDPGNA
                     QLMLGIAHYSQKEYAAARPFFERARQSDRHRQIAESYLQAIRAQS"
     gene            complement(1729..2331)
                     /locus_tag="VFL84_06900"
     CDS             complement(1729..2331)
                     /locus_tag="VFL84_06900"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET7608387.1"
                     /translation="MHALRIPIAALAGALFTTSMFWLLWSLIDTDFDVGERAIADRIE
                     FSRMRRDTEVATKRDEKVERERPPPTPETPRMALSAGGIDNNVASLSPIVDARGAMSR
                     MTMTAGSDRDVIPLVRVNPEYPQRALQRGLEGWVQVQFTITATGTVKDPVVVDAMPKN
                     VFDDAALKAIARWRYNPKVENGTAVERVGVQTRILFQLEQ"
     gene            complement(2355..2762)
                     /locus_tag="VFL84_06905"
     CDS             complement(2355..2762)
                     /locus_tag="VFL84_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7608388.1"
                     /translation="MQSRRHAPETEETGIDLAPMLDFTTNLLIFFIITTSFIKEAGTV
                     VTKPTAETAFGRESGNLMIAIRENGDIWMDRRRVELRDVRPMMERLHIERPDDTVVII
                     ADKASDAGVVAEVMDEVRLSGIQIMSIAADPAS"
     gene            complement(2783..3193)
                     /locus_tag="VFL84_06910"
     CDS             complement(2783..3193)
                     /locus_tag="VFL84_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007017798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7608389.1"
                     /translation="MRKRRRASGVDQEQTGIDLAPMLDFVTNLLIFFIITAVFVKEAG
                     LEVNRPTSFEQPQEDDSKGIQIQILENGEIWVDNRSVDVRAVRANVERMSAVNPEAGV
                     LILAQEHAPTGTLVEVVDQVHLGGIQNITFSTTQ"
     gene            complement(3196..3717)
                     /locus_tag="VFL84_06915"
     CDS             complement(3196..3717)
                     /locus_tag="VFL84_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7608390.1"
                     /translation="MLSQIPIVQGVIDLANDGGPWVPWIFASGVLMWALIVERFWYFT
                     MVLPKQAAELQAQWLARKDRTSWCARQIRSAMISRLNGSMTTGFPVMQVLVPMAPLLG
                     LIGTVSGMLEVFDSMALRGNADARSMASGVSAAMICTMTGLAVSITGLWPVHYFKTRA
                     VRETELLADKLSL"
     gene            complement(3710..5113)
                     /locus_tag="VFL84_06920"
     CDS             complement(3710..5113)
                     /locus_tag="VFL84_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7608391.1"
                     /translation="MTRRTMHCLLPLAAFVFAAPVLAQQQTPANTLEGLLKQVEQVGA
                     AADQAFNQHKAEFESKPQAEKDALLRNAEQQRNQLNQASARLSETYQANELRIGNLNQ
                     QLRDKAQSLGMAELFGLAHQAANDNSSILQQSMITTQFPTPPGETPRDEWMRQFAAST
                     AAPTAADLQRLWIEIQREMTASGQVAKYRTTVVQPNGQPQEAEVIRIGPFTALSNGQF
                     LAYLPNLREFNVLPRQLPGEFMSVAREFSNATSGYANAVVDAGRGSLLALYVERPTPI
                     ERIEAGETIGAIIVAVGVLGFLAFVFQLIHLVFVRIAVSSQLRHLDRPTPNNPLGRVL
                     LAFKGDKSRIEEDADVAELRITEAVLREVPKLERFQAFLRLCVAAGPLLGLIGTVVGM
                     ILTFQSITETGSSNPQLMANGIGIAMIATVLGLFVAIALLFANALLNSLSRGVVQILD
                     EQSAGMLAESIEKQRRA"
     gene            complement(5110..5805)
                     /locus_tag="VFL84_06925"
     CDS             complement(5110..5805)
                     /locus_tag="VFL84_06925"
                     /inference="COORDINATES: protein motif:HMM:NF023358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HET7608392.1"
                     /translation="MIKTTMRALLPLAAAILAAPAVAQNSQPAAEDAASLYARAIADV
                     ESLERYNRLLEEQVRSQEAEVASINRQLVEIETTTRQVQPLMQEMVNTIAQFVKLDIP
                     FLLEERTARVENLKRIMDRADVTLAEKYRLILDAYQRELEYGRTVEAYEGRLGTGADA
                     RTVEFARLGRVTLLYRTLDGQETGYWDAQKKEWVQDPSLGDAVEEALRRAKEDGPPEL
                     LIVPVPAPQEVRS"
     gene            complement(5802..6527)
                     /locus_tag="VFL84_06930"
     CDS             complement(5802..6527)
                     /locus_tag="VFL84_06930"
                     /inference="COORDINATES: protein motif:HMM:NF023358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HET7608393.1"
                     /translation="MPVLFVAPAGAQLQEALTQQVQVDKEAAQNQNELNQIRERTQDA
                     SGRYQQATADAESLERFNRQLEEQVNKQNEEVASIEKQLVDIETTTREVQPLMLRMVD
                     TLAEFIRLDVPFYIEERMVRLNELKDLMPRADIVISEKYRKILEAYQIELDYGRTFDT
                     YEGRLGTGPDARTVEFARLGRVSLMYRTLDGSETGYWDAQKKEWVADPSYREAVEQAL
                     EVAKGGDPDLLTVPVPPPQEVRS"
     gene            complement(7403..8641)
                     /locus_tag="VFL84_06935"
     CDS             complement(7403..8641)
                     /locus_tag="VFL84_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7608394.1"
                     /translation="MSASPATTASQHAATTAFSVLAAISFCHMLNDMMQSLLPAIYPI
                     LKDSHGLTFAQIGLLTFTFQVTASLLQPLIGFFTDKHPQPYSLSVGMGFSFAGLLLLS
                     LATSYPLLLAAAGLVGTGSAVLHPESSRVARMAAGNRPGLAQSLFQMGGNLGASIGPL
                     FAAVVVLRYGQGSVAWFALAAVVGMALLFAVGRWYSAHGMARMRAHAPRHRDPALSRA
                     RVAGALAILVALMLSKFVYTASVTSYYTFYLIERFGLSVRAAQLHLFVYLAAVAVGTV
                     VGGALGDRFGRKFVIWFSILGVLPFTVALPYANLYWTGVLSVPIGLILASAFPAIVVY
                     GQELVPLRVGTVAGLFFGFAFGLGGIGAALLGQLADVTGVQYVFKLCSFLPVIGLLAA
                     FLPARAGVGRPTTGVAPAAS"
     gene            8745..9326
                     /locus_tag="VFL84_06940"
     CDS             8745..9326
                     /locus_tag="VFL84_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608395.1"
                     /translation="MIEPASSGRAKCRSCDQPIAKGELRFGERQPNAFGEGEMTLWFH
                     LPCGAYSRPEPFLEAQGAAPADAGEDAASATAPGAVAGPELERLAVAARFGIEHRRVP
                     RLHGAERATSGRAHCRSCRELIAKGEWRLPLVFFEDYRFNPGGYVHARCARAYFETAE
                     LLDRVHHFSPELRDAELAEVAAAIAAGAAAPAQ"
     gene            9334..>10395
                     /locus_tag="VFL84_06945"
     CDS             9334..>10395
                     /locus_tag="VFL84_06945"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HET7608396.1"
                     /translation="MPLTLASPAQRNGARRGVDVLARLRERPPGIWYRGERVADVTTH
                     AAFRGGVATLAGLYDLQWARADECLFDSPTGGRKVARSFQLPRTSAELLGVSRAMAVV
                     ARHTHGMMGRVPDYLNRAASAYAAAAPFLAAADPRFGANAVRLHEELREADLSLTHSL
                     IPPQANRAAGPSAQADQFLAARIKKETDSGFVVRGCRMLATLPISDALMVLPSTLLKA
                     TADDAPYAFGFVIPNDAPGLKFICRESVDYSRSHFDHPLGSRYEEMDAVVVFDDVHVP
                     WENVLLYRDVERCNHANARTGAVVGMAHQVVIKNIAKSEFVLGLAALLVDAIAVEGFQ
                     HVQEKLAELWVTLETMRALL"
BASE COUNT         1764 a         3411 c         3300 g         1920 t
ORIGIN      
        1 cttcgacggt ctgcatcagc tcggtcagcc gctcgattcg ctgatcgcgc tcctccttga
       61 agaacggcac gtcgttctga acgaactgga cgagctcgtc gtacattcgc tgcagcagcg
      121 actggatgtc gacagccgtg gcgtccatgt tcgcgatctg ctcctcgagc gaggcgatct
      181 cccgctcttg cgagcgaagc tgctgctcga tctgcgtgtt gtaacgcgcc gtgatttgtg
      241 cgtcggccaa ggcccgcgcg taacgctcgg cggcggattc ctgagcgacg gcgacgccgg
      301 acactgccag cgccaacaaa ccgcaggcga tggaccattt attgttcgag gtgcgcgtca
      361 tgcaaagttc ctagaagaat cgatcaactc tgggcccgaa tggcctgcaa gtagctctcg
      421 gcgatctgcc gatgcctgtc cgactgccgc gcgcgctcga agaacggacg ggccgcggcg
      481 tactccttct ggctgtagtg cgcgatgccg agcatcagct gagcattgcc gggatctttg
      541 agctggccct tctcgacgcc gcgctggatc gcggcgatag cggcgtccca atcctcgcgc
      601 tgcacttgca cctcgccgag ccgtatgaag gtgtcgccgt tcggtgccat ctccgcagca
      661 cgctgcagcg gcccgacgga cttgtcgaac tcgccggccg caatccagca gtttgcgagc
      721 ttctcgtaca gcttgtcgtc gagcttcacg aggttcttct cgatcgcagc ctcgagcact
      781 tgtgcgccgc ggtatgggac gtcgttgaag agcaagagat cggcgagccg acgaacctcg
      841 gagtcctcgg tgacgaggcc cacgttgtgc gcgagctgca tgatcgcgag cgcgttcggg
      901 taatcctcca tttggccgta gatcgaagac agctgcatcc agtacgtctt cttcgacggc
      961 gcggcagcga tcagctgctg caagaccgga accgcctctt tgtattcctc acgctgcaag
     1021 tacagcgcgg agagcatgct gagccagctc tcgttcggct tggcgggatc catgagctcg
     1081 acggccttct tcgagggcgc cagcgcacgg ttgaagtcct cctgctggta gtacgcgacg
     1141 gcgagcaggt agtacgccgc cgagttcggg ttggcggccg tcttgaacca ctcttcgagc
     1201 gccgccgcgc cctctttcca tttctcttcc tgcatgtaga gctgggcgcg ctgataacgc
     1261 agctcatcga tctgctgcgc attcaagccg cccgagtcga tggccttctg gatatggccg
     1321 cgcgcttctt cgtacttctc ttgtgcatac gagatgctga acatgatccg ctcgacggtg
     1381 ccgcgctcgt acgggctcag cctgtcgaga ttcagggtgt tgatccttgc cgcggccccc
     1441 tgatagttct ccatgttcag gagctcgatc gcttcgttca ggactttgcc cgtctgcgcg
     1501 tcgatgctat tggcgcctgc ggccggggcc tcttcttcct gatttctcgc ggcggcgcgg
     1561 ctcgtgccct ggttcgacgc attctggccc tgcgggacac cgccttgggc aagggccgtc
     1621 ccggcgagcg cgaagctcac cgcgagcgcg attccggtca tgaccaaccg caacgaaccg
     1681 tttgcttgct gttccatggc tctctcagct cctgtcggac gaatccgttt actgctcgag
     1741 ttggaaaaga attcgcgtct gaacgccgac ccgctcgact gcggtgccgt tctcgacttt
     1801 cgggttgtag cgccagcgcg cgatcgcctt cagggccgcg tcgtcgaaca cgttcttcgg
     1861 catcgcgtcg acgacgacgg ggtctttgac cgtgcccgtc gccgtgatcg tgaactgcac
     1921 ctgcacccag ccttcgagcc cgcgctgcag cgctcgctgt ggatattcgg gattgacgcg
     1981 aaccagcgga atgacgtcgc ggtccgaacc ggccgtcatc gtcatgcgcg acatcgcgcc
     2041 gcgcgcgtcg acgatgggcg acagtgaagc gacgttgttg tcgatgccgc ccgccgacaa
     2101 cgccatgcgc ggcgtctccg gcgtcggcgg cggccgctcg cgctcgacct tctcatcgcg
     2161 cttcgtcgcg acctcggtgt cgcggcgcat gcgcgagaat tcgatgcggt ccgctatggc
     2221 gcgttcgccg acgtcgaaat cggtatcgat gaggctccag agcagccaga acatcgacgt
     2281 cgtgaacaat gcgccggcaa gggcagcgat gggaatcctc agtgcgtgca tgctgattgc
     2341 ctcctgcgtt ctcgttagct ggccggatcc gccgcaatcg acatgatctg aataccggag
     2401 aggcgaacct cgtccatgac ctcggcgacg acgccggcgt ccgacgcctt gtcggcgatg
     2461 atcacgaccg tatcgtcggg gcgctcgatg tgcagccgct ccatcatcgg gcgaacgtcg
     2521 cgcagctcga cgcggcggcg atccatccag atgtcgccgt tctcgcgaat cgcgatcatc
     2581 aagttgccgg actcgcggcc gaacgccgtc tcggcggtcg gcttcgtgac gacggtgccg
     2641 gcttccttga tgaaggacgt cgtgatgatg aagaaaatca gaagattcgt cgtgaagtcg
     2701 agcatcggcg cgaggtcgat acccgtttcc tctgtttccg gcgcgtgtcg tcgcgactgc
     2761 atgattctct tccctctcgc cgctactgcg tcgtgctgaa cgtgatgttt tggatgccgc
     2821 cgaggtgcac ttggtcgacg acctcgacca atgtcccggt cggcgcgtgc tcttgtgcga
     2881 gaatcagtac gccggcttcc gggttcacgg cgctcatccg ctcgacgttc gcgcgcacgg
     2941 cccgcacgtc gacggagcgg ttatcgaccc atatttcgcc gttctcgagg atctggatct
     3001 ggatgccctt cgagtcatcc tcttgcggct gctcgaacga cgtggggcga ttgacctcga
     3061 ggcccgcctc cttgacgaac actgccgtga tgatgaaaaa gatcagcagg ttcgtgacga
     3121 agtcgagcat cggcgctagg tcgatgcccg tctgctcctg atctactccc gaggcgcgtc
     3181 gtcgctttcg catggttaca agctcaattt gtcggcgagg agctccgttt cacggaccgc
     3241 tcgcgtcttg aagtagtgga ccggccacag cccggtgatg ctgacggcga gacccgtcat
     3301 cgtgcagatc atggcggcgg acacgccgct cgccatgctg cgcgcgtcgg cgttgccgcg
     3361 cagcgccatc gagtcgaaga cctcgagcat gcccgagacc gtgccgatga ggccgagcaa
     3421 cggcgccatc ggcacgagta cctgcatgac cggaaagccc gtcgtcatcg atccgttcaa
     3481 ccgcgagatc atggccgagc ggatttgccg cgcgcaccac gaggtccggt ccttgcgggc
     3541 gagccattgg gcctggagct ccgcggcctg cttcggcagc accatcgtga agtaccagaa
     3601 ccgctcgacg atcagcgccc acatcagcac gccggaggcg aagatccacg ggacccaggg
     3661 gccaccatcg ttggccagat cgatgacgcc ctgaacgatt ggtatttggc taagcacggc
     3721 gttgtttctc gatgctctcc gccagcatgc cggcactctg ctcgtcgagg atttggacga
     3781 cgccgcgcga caacgagttc aacagcgcgt tggcgaacag gagggcaatg gccacgaaca
     3841 ggccgagcac cgtcgcgatc atggcaatgc cgatgccgtt ggccatgagc tgcgggttgc
     3901 tcgagcccgt ctccgtgatg ctctggaacg tcaagatcat gccgacgacc gtgccgatca
     3961 ggccgagcaa cggaccggcg gcgacgcaca agcgtaggaa cgcctggaac cgctcgagct
     4021 tcggcacctc gcgcagcacg gcctccgtga tccgcagctc ggcgacgtcg gcgtcctcct
     4081 cgatcctgct cttgtcgccc ttgaacgcga gcagcacgcg gccgagcggg ttgttcggcg
     4141 tggggcggtc cagatgtctc agctgactgc tgactgcgat gcggacgaag acgaggtgga
     4201 tgagctggaa gacgaacgcc aggaacccaa gcacgccgac ggcgacgatg atcgcgccga
     4261 tcgtctcgcc tgcctcgatg cgctcgatcg gagtcggccg ctcgacgtac agcgccagca
     4321 gcgagccgcg gcctgcgtcg acgactgcgt tggcgtagcc cgaggtcgca ttcgagaact
     4381 cgcgcgcgac gctcatgaac tcgcccggca gctggcgcgg cagcacgttg aactcgcgca
     4441 gattcggcag gtacgccaag aactggccat tcgagagcgc cgtgaacggc cctatccgaa
     4501 tgacctcggc ctcttgcggc tggccgttcg gctgcacgac ggtcgtgcga tatttcgcga
     4561 cctgcccgct cgccgtcatc tcgcgttgga tctcgatcca caagcgctga aggtcggcgg
     4621 ccgtgggcgc cgcggtcgac gcggcaaact gccgcatcca ctcgtcacgc ggagtctcgc
     4681 cgggaggcgt tgggaactgc gtcgtgatca tcgactgctg caagatcgac gagttgtcgt
     4741 tggcggcctg gtgcgccaga ccgaacagct cggccatccc gagcgactgc gctttgtcgc
     4801 gcagctgctg gttcaagttg ccgatacgca gctcgtttgc ctggtaggtc tccgaaagtc
     4861 gagcggacgc ttggttgagc tggttacgct gttgctcggc gttacgcagc agcgcgtcct
     4921 tctcggcttg cggcttcgac tcgaactcgg ctttgtgctg attgaaagcc tggtccgcag
     4981 cggctcctac ttgctcgacc tgcttcagta ggccttcgag cgtgttggcc ggcgtctgct
     5041 gttgagcaag gaccggggcg gcgaatacga aagctgccag cggcagcaag caatgcatag
     5101 ttcttctggt cacgaccgca cctcctgcgg cgctgggaca ggtacgatca acaattcggg
     5161 cgggccgtct tccttcgcgc ggcgaagcgc ctcttcgacg gcatcgccta agctcgggtc
     5221 ttgaacccat tctttcttct gcgcatccca atagcccgtc tcctggccgt cgagcgtgcg
     5281 gtacagcagc gtgactcggc cgagccgtgc gaactcgacg gtccgcgcat ccgcgccggt
     5341 gccgagccgc ccttcgtaag cctcgaccgt gcggccgtac tcgagctcgc gctgataggc
     5401 atcgagaatc aggcggtatt tctctgcgag cgtgacgtcg gcgcggtcca tgatccgctt
     5461 caaattctcc acgcgcgccg tgcgctcctc gagcaggaac gggatatcga gcttcacgaa
     5521 ctgagcgatc gtgttgacca tctcctgcat gagcggctga acctgccgcg tcgtcgtctc
     5581 gatctcgacg agctggcggt tgatcgacgc gacctccgcc tcctgcgagc ggacctgctc
     5641 ttcgagcagc cggttgtacc gctcgagact ctccacgtcc gcgatcgcac gtgcgtaaag
     5701 gctcgccgcg tcctccgccg ccggttgact gttttgcgcc accgcagggg cagcaagtat
     5761 cgccgccgcg agcggcagca gagctctcat tgttgttttg atcacgaccg cacctcctgc
     5821 ggcggtggaa caggtacggt cagcaaatcg gggtcgccgc ctttcgcgac ttcgagcgct
     5881 tgctcgacgg cctctctgta gctcgggtct gcgacccatt cctttttctg cgcatcccaa
     5941 tagcccgtct ccgagccgtc gagcgtgcgg tacatcaagg acactcgacc gagccgcgcg
     6001 aactcgacgg tgcgcgcgtc gggacccgtc ccgagccggc cttcgtacgt atcgaaagta
     6061 cggccgtagt cgagctcgat ttgataggcc tcgagaatct ttcggtactt ctcggagatc
     6121 actatatccg cgcggggcat gaggtccttc agctcgttca agcgaaccat gcgctcttcg
     6181 atatagaacg gcacatcgag cctgatgaat tcggcgagcg tgtcgaccat gcgcagcatc
     6241 agcggctgca cctcgcgcgt cgtcgtctcg atgtcgacga gctgcttctc gatcgacgcg
     6301 acttcctcgt tttgcttgtt gacttgctcc tcgagctgcc tgttgaaacg ctcgaggctt
     6361 tcggcatctg cggtcgcttg ctgatagcgg ccggaagcat cttgtgtgcg ctcgcggatt
     6421 tgattgagct cgttttgatt ttgggcggct tctttgtcca cctgaacctg ctgagtgagg
     6481 gcttcttgca actgtgcgcc ggcaggtgcg acgaacagga ccggcacgag aacctcgcag
     6541 aggcgtatcc aacgcgcgcc agtagctcga gctactttgg gtctactggg gatcatgaca
     6601 aacggcagcc tcctgaaatg atttgcacga ctcgttgctt gcacgactcg cgccaaacgg
     6661 cgttgatcga agagtcatca taaaaaccca aaacgactag gcagcctccg cgtcagcgaa
     6721 gtgccgccca aactccaatg attccacgaa ggagtgcgcg cccacaacac gccgagcggc
     6781 accacttgcc gcacgcgcag ccgtccgatg agtcgaatca ccgtacacaa gcacccccct
     6841 ttgccagacc agaaggaatc ctcgatccct tcgccccggc catagcaacc caatctagtt
     6901 ggagttatcg ccgtctgcat cctacgggga cacgcgccta tccggccgcc cccgccgccg
     6961 gcgttgacga gccaggctcc acgcagcgac tgagtcgtag tgtagtcaaa gtctctcgcg
     7021 catgcctaga gggcaaaggc gcgcgccggg ccctctcgac agaatgtgcc gcttgcagtg
     7081 agacgtcgtg cggccggcac gggcgcgctg ccacttcatc gtaggcgaac tcgtgaggac
     7141 ggattttcgc gtgcgaagcg gacgacgcat ccgtaccgga agttccgccg ctgcccaaaa
     7201 aaatcgctga aacgcgcatc tcgtccgtga tatcgctgcc gcgaccgcgg ccacttcgcc
     7261 ttgtatgcgc gtttgacgaa tgaagtggcg cactggttcc aatcttcccg ctcgctgttt
     7321 cctctgtgcc tcggcatcgc cggcgggttc acatatatat aggtaggcgt tgtaggcgtt
     7381 gcaccggaac gcgcgcggcg cttcagctgg cagccggggc gactcccgtc gtcggccgcc
     7441 cgactccggc ccgcgccggc aggaaggccg cgagcagacc gatgaccggt aggaacgagc
     7501 agagcttgaa gacgtactgc acgcccgtga cgtcggcgag ctgcccgagc agcgcagcgc
     7561 cgatcccgcc gaggccgaac gcgaatccga agaacaggcc ggcgacggtc ccgactcgca
     7621 gcggcacgag ctcctgaccg tacacgacga ttgcgggaaa tgccgaggcg aggatgagcc
     7681 cgatcggcac gctcagcacg ccggtccagt acaaattcgc gtacggcagc gcgaccgtga
     7741 acggcagaac tccgaggatc gagaaccaga tgacgaattt gcggccgaac cggtcgccga
     7801 gtgcgccgcc gaccacggtg ccgacggcga ccgccgcgag gtaaacgaac aagtgcagct
     7861 gcgcggcgcg caccgaaagg ccgaagcgct cgatcaggta gaacgtgtaa tagctcgtga
     7921 cgctcgcggt atagacgaac ttcgataaca tcagcgcgac gaggatcgcg agcgcgccgg
     7981 ccacgcgtgc acgcgacaac gccgggtcgc ggtgacgcgg cgcatgcgcg cgcattcgcg
     8041 ccatgccgtg cgcggagtac cagcggccga cggcgaacag caacgccatt ccgacgacgg
     8101 cggcgagcgc gaaccaagcg acgctgccct gcccgtagcg cagcaccacg accgcggcga
     8161 acagcggccc gatcgatgcg ccgagattgc cgcccatttg gaacagcgat tgcgcgagcc
     8221 ccggcctgtt gccggcggcc attcgagcga cgcgcgagga ttcgggatgc agaaccgccg
     8281 accctgttcc cacgagcccc gcggctgcca acagcagcgg gtagctcgtt gccagtgaca
     8341 gcagcagaag gcccgcgaac gagaagccca tgccgacgga cagcgagtac ggctgcgggt
     8401 gcttgtccgt gaagaatccg atcaacggct gcaacagcga tgccgtcacc tgaaacgtga
     8461 acgtcagtag gccgatctgc gcgaacgtca ggccgtggct gtctttgagg atcggataga
     8521 tcgccggcag cagcgactgc atcatgtcgt tcaacatgtg gcagaagctg atggcggcga
     8581 gcacggagaa cgccgtcgtg gcggcgtgtt gcgatgctgt cgttgctggc gaggcgctca
     8641 ctgtgaaggc tcgatgacta cgtgtccgaa gcctagctta agcgaagcgg cggcgccgag
     8701 gagccgcggc cgcgagcaca ccctataatt ccgcggtggc acacgtgatc gaacccgcct
     8761 cgagcggccg cgccaaatgc cgcagctgcg atcaaccgat cgcgaaaggc gagctgcggt
     8821 tcggcgaacg tcaaccgaac gcgttcggcg aaggcgaaat gacactgtgg ttccatctgc
     8881 cctgcggcgc ctactcgcgg cccgagccgt tcctcgaggc gcagggagcc gcgcctgcgg
     8941 acgccggcga ggacgccgcc agcgcaacgg cgccaggagc ggttgccggt cccgagctcg
     9001 agcggctcgc ggtggcggcg aggttcggca tcgagcaccg gcgcgtgccg cgcttgcacg
     9061 gcgccgagcg cgcgacgtcg ggccgtgcgc actgccgcag ctgccgcgag ctgatcgcga
     9121 agggcgaatg gcggctgccg ctcgtcttct tcgaggacta tcgcttcaat cccggcggct
     9181 atgttcacgc gcgctgcgcc cgcgcgtact tcgagacggc cgagctactc gaccgcgtcc
     9241 accacttctc tccggagctt cgcgacgccg agctcgccga ggtcgccgcc gcgatcgcgg
     9301 ccggtgctgc ggcgccggcg caatgaagcg cctgtgccgc tgacgctcgc ctcaccggca
     9361 cagcgaaacg gcgcccgccg cggcgtcgac gtgctggcgc gcctgcgcga gcgaccgccc
     9421 gggatctggt accggggcga acgcgtcgcc gacgtcacga cgcacgccgc cttccgcggc
     9481 ggagtcgcaa cgctcgccgg gctttacgac ttgcagtggg cgcgcgccga cgaatgcctc
     9541 ttcgattcgc cgaccggcgg ccgcaaggtt gcgcgctcgt tccagctgcc gcgcacttcg
     9601 gcggagctct tgggcgtgag ccgcgcgatg gccgtcgttg cgcggcacac gcacggcatg
     9661 atgggccgcg tcccggacta tctgaatcgc gcggcctcgg cctacgccgc cgcggcgccg
     9721 ttcctcgcgg cggcggaccc gcgcttcggc gcgaacgccg tgcgcctgca cgaggagctg
     9781 cgcgaggcgg atctatcgct gactcattcg ttgatacccc cgcaagcgaa ccgcgccgcc
     9841 gggccgtcgg ctcaggccga ccaattcctt gccgcacgca ttaaaaagga gaccgattcc
     9901 ggtttcgtcg tgcgcggttg ccgcatgctc gccacgctgc cgatctcgga cgcgctgatg
     9961 gtcttgccgt cgacgctgct gaaggcgact gccgacgatg ctccgtacgc gttcggcttc
    10021 gtgattccaa acgacgcgcc ggggctcaaa ttcatctgcc gcgagagcgt cgattacagc
    10081 cgttcgcatt tcgaccatcc gctcggctcg cgttacgagg agatggacgc cgtcgtcgtg
    10141 ttcgacgacg ttcatgtgcc gtgggagaac gttctgctct accgcgacgt cgagcgctgc
    10201 aaccacgcga acgcgcgcac gggagccgtc gtcggcatgg cacaccaggt cgtcatcaag
    10261 aacatcgcga agtccgagtt cgtgctcggg ctcgcggcgc tgctcgtcga cgcgatcgcc
    10321 gtcgaaggct ttcagcacgt ccaagagaag ctcgcggagc tctgggtcac gctcgagacg
    10381 atgcgcgcgc tgctg
//
LOCUS       DASSNN010000377         4263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1451439, whole genome shotgun sequence.
ACCESSION   DASSNN010000377 DASSNN010000000
VERSION     DASSNN010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451439"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFL84_06950"
     CDS             complement(<1..627)
                     /locus_tag="VFL84_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608397.1"
                     /translation="MRRSAWQPVLAAAACGALVVAGSSTPSLQARPVPAVGGTPSAPP
                     DSSDGGRWLEATLTALDDPKEVRMPYAALGELGGAADVAAAYAFRARAGQTLEITLRR
                     DRASNGSIYVELFRVVDVLGQGLRERLTSLRPSATSIRARLPSDGTYQVLVQTGATGG
                     GHYGLTLELDAALPFPVVGGESSSIRSLFGASRDAGRRNHEGVDIFVRR"
     gene            complement(624..2762)
                     /locus_tag="VFL84_06955"
     CDS             complement(624..2762)
                     /locus_tag="VFL84_06955"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HET7608398.1"
                     /translation="MGITRKTKSALSEPIERPASARYGDQAVELGAGGETHQRAVDDH
                     ELTTQQGTPIADDQNTLRVGARGPALLEDFHFREKLFHFDHERIPERVVNARGFGAHG
                     FFETYDSLADVTSADLFQRAGEVTPAFVRFSTAMGSRGSFDLARDVRGFAVKLYTQRG
                     NWDLIGNNMPVFFIQDPIKFPDLVHAAKPAPDRDFPQAQSAHDNFWDFMSLMPESLHM
                     VLWLMSDRGIPRSFRFMEGFGVHTFRLVNQAGVSTFVKFHWKPKLGLQSVSFQEAVKI
                     NGVDPDFHRRDLWEAIEAGDFPEWELGLQLFGADTAALLPFDVLDATKLITEEQVPIR
                     RVGRLVLDRNVKNAFAETEQVAFGTHNIVPGIEFSNDPVLQGRNFSYLDAQVKRLGSP
                     NYTQLPINAPQCPFRHFQQDGNMALFNPSGRANYAPNSWLAGRGAAEAASGGFASFAE
                     DVSGAKIRARPESFADHYNQARQFYVSQTPLERQHIAAALVFDLSKVETPAIRSRVVA
                     HLLNVDDRLAMNVAEGLRLESLPAPATRVVPARDDLAESPPLSIMRSAAQTFAGRRLG
                     VLVTDGVDSSFLRQLENEFNDVGATIELVAPAIGGIKSEDGAWIEARKSLATAPSVLF
                     DAVAIVVSPQASKDIAKSPLASAFVADAYAHCKFIAYTADARPLLERALAGSPLDGGC
                     KQVETGIAAAQFASQCSQLRFWAREPVLGL"
     gene            complement(2940..3104)
                     /locus_tag="VFL84_06960"
     CDS             complement(2940..3104)
                     /locus_tag="VFL84_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018948711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HET7608399.1"
                     /translation="MIYYALVFLVVALIAGVLGFSGIAGTAANIAWILFVVGLVLAVV
                     FFVRGRGSSI"
     gene            3256..4104
                     /locus_tag="VFL84_06965"
     CDS             3256..4104
                     /locus_tag="VFL84_06965"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET7608400.1"
                     /translation="MNVSGVWRKQPKMAVIAAAIAVALGTAAQQQEPNRENANRPQTS
                     ETQIARSDAGIQSLDQYLKSKTLRVSKVVGMELMTRSGENLGEVEDILRGTAPGQDMQ
                     LVVKTGGVGADEKLVALPFDEVQINAKGDELYTMRTKEQLASAPAVQLERRTAGNATP
                     AQRCAAEANRDAGRSGAASTPSTASNQQRMADLVGAEVVGSDGDKVGEVDDIVLSTAG
                     ADSIRAVLQVGGVAGIGEKRVSLPLTQITVERPADDEPTLRVAMDADALQRLPEYEYE
                     DQTSVL"
BASE COUNT          743 a         1436 c         1430 g          654 t
ORIGIN      
        1 ccgccgcacg aatatgtcca cgccctcgtg gttgcgcctg ccggcgtctc gtgacgctcc
       61 gaacaggctg cggatcgagc tcgactcgcc gcccacgaca gggaacggca gtgcggcgtc
      121 gagctcgagc gtcaagccgt aatgcccgcc acccgtcgcg cccgtctgca cgagcacctg
      181 ataggtgccg tcgctcggca acctcgcgcg aatgctcgtc gcgctcggcc gcagggaggt
      241 cagccgctcg cgcaggccct gcccgaggac gtcgacgacg cgaaacagct cgacatagat
      301 cgacccgttc gatgcgcggt cgcggcgcaa cgtgatctcg agcgtttggc cggcccgcgc
      361 ccggaacgcg taggcggccg cgacgtcggc cgcgccgccg agctcgccga gcgccgcata
      421 cggcatgcgc acctctttag gatcgtcgag cgccgtcagc gtcgcttcga gccagcggcc
      481 gccgtcgctc gagtccggcg gcgcgctcgg cgtcccgccg acggccggca ctggacgcgc
      541 ctgcagcgac ggcgtgctcg agccggcgac gaccagcgcg ccgcatgccg cggcggcgag
      601 gacgggctgc cacgcactgc gcctcataac ccgagcaccg gctcgcgcgc ccagaagcgc
      661 agctgcgagc actgcgacgc gaattgcgcg gccgcaatgc cggtctcgac ctgcttgcag
      721 ccgccgtcga gcgggctgcc cgccagggcc cgctcgagca gcgggcgagc atcggccgtg
      781 taggcgatga atttgcagtg cgcgtaggca tccgcgacaa acgcggacgc caaggggctc
      841 ttggcgatgt ccttgctggc ctgcggcgag acgacgatcg cgaccgcgtc gaacaacacc
      901 gacggcgccg tggcaagcga cttgcgcgcc tctatccagg cgccgtcctc gctcttgatg
      961 ccgccgatgg ccggggcgac gagctcgatc gtggcgccga cgtcgttgaa ctcgttctcg
     1021 agctgacgca ggaagctgct gtcgacgccg tcggtgacga gcacgccgag ccggcggccg
     1081 gcgaacgtct gcgcggcgct gcgcatgatg ctgagcggcg gcgactcggc gaggtcgtct
     1141 cgcgccggca cgacgcgcgt cgcaggcgct ggcagcgatt cgaggcgcaa gccttccgct
     1201 acgttcatcg cgagccggtc gtcgacgttc agcagatgag cgacgacgcg cgagcggatc
     1261 gccggcgtct cgaccttgct gagatcgaag acgagcgccg ccgcgatgtg ctggcgctcg
     1321 agcggtgtct ggctcacgta gaactgccgc gcctgattgt agtggtccgc gaagctctcg
     1381 ggccgcgcgc ggatcttcgc gccgctcacg tcttcggcaa acgaagcgaa gccgccgctc
     1441 gcggcctccg cggcgccacg gccggcgagc cacgagttcg gcgcatagtt cgcgcgcccg
     1501 ctcggattga acagcgccat gttgccgtct tgctgaaagt gcctgaacgg gcactgcggc
     1561 gcgttgatcg gcagctgcgt gtagttcggg cttccgagcc gcttcacttg cgcatcgaga
     1621 tacgagaagt tgcgcccctg taggacgggg tcgttgctga actcgatgcc ggggacgatg
     1681 ttgtgcgtcc cgaacgcgac ttgctcggtc tccgcgaacg cattcttgac gttgcggtcg
     1741 agcacgagcc ggccgactcg gcgaatcggc acttgctcct cggtgatcag cttcgtcgcg
     1801 tcgagcacgt cgaacggcag cagcgcggcc gtgtcggcgc cgaacagctg caagcccagc
     1861 tcccactccg ggaaatcgcc ggcctcgatc gcttcccaga gatcgcggcg gtggaagtcc
     1921 ggatcgacgc cgttgatctt cacggcctct tgaaacgaca ccgattgcag cccgagcttc
     1981 ggcttccaat ggaacttgac gaacgtcgat acgccggctt ggttgacgag ccggaacgtg
     2041 tgcacgccga agccctccat gaaccgaaac gagcgcggaa tgccgcggtc cgacatcagc
     2101 cacagcacca tgtgcaggct ctcgggcatc agcgacatga agtcccagaa attgtcgtgc
     2161 gccgattgag cctgcggaaa gtcgcggtcg ggtgccggct tcgcggcgtg cactagatcc
     2221 gggaacttga tcggatcctg aatgaagaac accggcatgt tgttgccgat gaggtcccaa
     2281 ttgccgcgtt gcgtgtacag cttgaccgcg aaaccgcgca cgtcgcgcgc gagatcgaac
     2341 gagccgcggc tgcccattgc cgtcgagaac cgcacgaacg cgggcgtgac ctcgcctgct
     2401 cgttgaaaaa gatccgcgct cgtgacatcc gccaacgaat cgtatgtctc gaagaatccg
     2461 tgcgcgccga agccgcgcgc attcacgact cgctcgggta ttcgctcgtg gtcgaaatgg
     2521 aaaagctttt cgcgaaagtg aaaatcttcg agcagcgccg gtcctcgcgc gccgacgcgc
     2581 agcgtgtttt ggtcgtccgc tatcggcgtt ccttgctgcg tcgtgagctc gtggtcgtcg
     2641 acggcgcgct gatgggtttc gccccctgcc ccgagctcga cggcctggtc gccgtaacgc
     2701 gcgctcgccg gccgctctat cggctcactg agcgcactct tcgtcttccg cgtgatcccc
     2761 attcgaagtc gccgttcgtg ctcccgcgcg cgcatcggcc gcgcgcggga gcccgcaggt
     2821 atgcgccgcc gaaagccgcg cggacacgct ccggggccac gtcaatcgcc gcgcgtcggc
     2881 gtgctttcac gcgggctgca attgcagccg ccgtgcacgc aaccctgatc ggccactgct
     2941 caaatgctcg acccgcggcc acgcacgaag aacacgaccg cgagcaccag gccgactacg
     3001 aacaagatcc aagcgatatt ggcagcggtt ccggcgatgc ccgagaagcc gagcactccc
     3061 gcgattagcg ctaccacgag gaacacgagc gcgtaataga tcatggggac actcctattg
     3121 cccaacctgc agatggctct tttgcaagct ctgtgccacg ccacggagcc atctagtggc
     3181 atatgtcttg cacctattcg gcccgtccgg cggcttggca gccgcggttt cgatttgcaa
     3241 caaggaggtg aactcatgaa cgtttccgga gtttggcgaa agcaacccaa gatggccgtc
     3301 atcgcggccg cgatcgcagt tgcgctcggc accgctgcgc aacagcagga gccgaaccgc
     3361 gagaacgcga atcgcccgca gacgagtgag acgcagattg cgcgtagcga cgccggcatc
     3421 cagtcgctcg accagtacct caagagcaag acgctgcgcg tcagcaaagt cgtcggcatg
     3481 gagctcatga cccgtagcgg agagaacctc ggcgaagtgg aggacatcct ccgcggcacc
     3541 gcgccgggtc aagacatgca gctggtcgtg aagacgggcg gcgtcggcgc cgatgagaag
     3601 ctcgtcgcgc tgccgttcga cgaagtgcag atcaacgcga agggcgacga gctttacacg
     3661 atgcgcacga aggagcagct cgcgtccgcg ccggccgtcc agctcgagcg gcgcacggcg
     3721 ggcaacgcga ctccagcgca gcgctgcgca gccgaagcga accgcgatgc ggggcgaagc
     3781 ggcgcggcgt ccacgccgtc gacggcctcg aaccagcaac gaatggccga tctcgttgga
     3841 gccgaggtcg tcggcagcga cggcgacaag gtcggcgagg tcgacgacat cgtcctctcg
     3901 acggccggcg cagacagcat ccgcgcggtg ctgcaagtcg gcggcgtcgc gggcatcggc
     3961 gagaagcgag tctcgctgcc gctcacgcag atcacggtcg agcgtcccgc ggacgacgag
     4021 ccgactctgc gagtcgcgat ggacgcggat gcgctgcagc gtctgcccga gtacgagtac
     4081 gaggaccaga cgtcggtgct ctagcgtgtg ctcgaggagc cggccgcgga accgcggccg
     4141 gctcctcggt ttaggcgccg gcggacgaac gcgggcgccg aggataggag agacatgagc
     4201 tccaaacgaa cgaccgatca cgcgaagatt cggcagtggg ccgaagcgcg caacggaaag
     4261 cct
//
LOCUS       DASSNN010000378         3802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1815774, whole genome shotgun sequence.
ACCESSION   DASSNN010000378 DASSNN010000000
VERSION     DASSNN010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815774"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFL84_06970"
     CDS             complement(<1..740)
                     /locus_tag="VFL84_06970"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HET7608401.1"
                     /translation="MAENPYAAPRTHVEDTPTTLPDGPFIPEGRRVPAGHGWRWIADA
                     WGFMGEQRWTFIGVFLLMMVIQIAAQLVPIVGPLVVALFYPVILGGFTLGCEAVRLGQ
                     RFEVGHLFAGFQRHTGKLVAVGALSLAFGVVAVIIMVMIIGVSALPLLVGGAQPTPEE
                     VMSMMLPLMLGVLVVLAVSLPVSMAFLFAIPLIVLRDSAVGAALKTSFFACLKNVLPF
                     LVWSLAMLVLGFVAAIPLFLGFLLLAPVS"
     gene            complement(765..2249)
                     /locus_tag="VFL84_06975"
     CDS             complement(765..2249)
                     /locus_tag="VFL84_06975"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7608402.1"
                     /translation="MLLLAAVTARAQITSNPLRAPIEKRGLAVEVRDVVRLPETRNLR
                     PVEQDVTPTGYARVSFVRDAPDGRRFANDSRGFLYIVDGARTSLYADVAAAFPRAFYG
                     RLESGFIGFTFHPEFERNGLWYSVHLESANGNPARPDFIPPGFGPNDVTYHNVLTEWH
                     ATDPKASTFEGTRRELLRYAHVVQFSSHPLGAVEFNPTARPGSPDYGLIYTSGSDLGF
                     SNGGGPNASNPSATQRLDSLITAILRIDPRSPRETGGPKGLGDYTIPADNKLQADGDP
                     KTLGEIYAYGFRNAHRLAWDLADGALFVGDIGMNHIEEINIVRNGGNYGWMKREGYFE
                     NGVIRPGGALNQLYELPADVLKGRGRDEFTYPVAIYDHDEGVAITGVFPYRGRIEALR
                     GKLVFGDVNRGRLFAVDLAAAHAADDGIPETVAPIEEIQLYVRGAAGQRIYTSFRELV
                     EGTMGTSLSRTDLHLSRSADGELFLTSRQDGMIRMLVPDSGRDR"
     gene            complement(2431..3291)
                     /locus_tag="VFL84_06980"
     CDS             complement(2431..3291)
                     /locus_tag="VFL84_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7608403.1"
                     /translation="MRLWSVEGNRQRLDGGAMFGNVPRNLWAKWLTPDGENRLEFACR
                     CLLVQDLGGKTVLFETGIGAFFEPKLRARYGVHESEHMLLRSLEARGVRHQDVDAVVL
                     SHLHFDHAGGLLSAYEESRPPELLFPRARYIVSKAAWARATAPHPRDRASYIADLPAL
                     LRKSGRLEILDRPQSTLLGPAVRFDFSDGHTPGLALAEIGGEGGVAFCSDLIPGRPWV
                     HLPVTMGYDRYPELLIDEKQGFLEDKISRRVRLFFTHDLECAAALPVRGDDGRFGVTA
                     EQRTLEGEEL"
     gene            complement(3272..>3802)
                     /locus_tag="VFL84_06985"
     CDS             complement(3272..>3802)
                     /locus_tag="VFL84_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7608404.1"
                     /translation="VPQDTMLFGASARENIRYGRPNASDADIEAAARAAEADEFIRAL
                     PQGYDTFLGERGLRLSGGQRQRIAIARAILKDPPILLLDEATSSLDAASELLVQQALE
                     KLMAHRTTIIIAHRLATVQKADRIVVMDAGRVVASGTHAQLTSASPLYARLAQLQFGA
                     AGQPEPEEKARAALVR"
BASE COUNT          628 a         1297 c         1300 g          577 t
ORIGIN      
        1 gagaccggag cgagtaggag aaagcccagg aacaacggta tcgccgcaac gaagcccagc
       61 acgagcatgg ccaggctcca aacaagaaac ggcaagacgt tcttgaggca cgcgaagaag
      121 ctcgtcttca gcgccgcccc aacggccgag tcccgcagca cgatcagcgg tatcgcgaac
      181 aagaatgcca tcgagaccgg taggctcacg gccaagacca cgagcacgcc cagcatcagc
      241 ggcagcatca tgctcatgac ttcctccggc gtcggctgcg cgccgccgac gagcagcggc
      301 agcgccgaga cgccgatgat catgaccatg atgatgaccg cgacgacgcc gaacgcgagc
      361 gagagcgcgc cgaccgcgac gagcttgccc gtgtgccgct gaaagcccgc gaacagatgc
      421 ccgacctcga accgctgccc gaggcgcacg gcttcgcagc cgagcgtgaa gccgcccagg
      481 atgacggggt aaaacaaggc gacgaccagc gggccgacga tcggcacgag ttgcgccgcg
      541 atctggatca ccatcatcag caggaacacg ccgatgaacg tccagcgctg ctcgcccatg
      601 aagccccaag cgtcggcgat ccagcgccag ccgtggccgg ccggcacgcg gcgcccttcg
      661 ggtatgaacg ggccgtcggg cagcgtcgtc ggcgtgtctt cgacgtgcgt gcgtggcgcg
      721 gcgtacggat tttcggccat tcggcttctc cttccgagag ctgactagcg gtcgcgcccc
      781 gagtccggca cgagcatgcg aatcatgccg tcctgacgtg aggtcaggaa caattcgccg
      841 tcggcgctgc ggctcaagtg caagtcggtg cgcgagagcg acgtgcccat cgttccttcg
      901 acgagctcgc ggaacgaggt atagatgcgc tgccccgccg cgccgcgcac gtagagctgg
      961 atctcctcga tcggcgcgac ggtctctggg atgccgtcgt cggccgcatg cgccgcggcg
     1021 agatcgacgg cgaacaaccg gccgcgattg acgtcgccga atacgagctt gccgcgcagc
     1081 gcttcgatgc ggccgcggta cggaaagacg cccgtgatcg cgacgccctc gtcgtggtcg
     1141 tagatcgcga cgggatacgt gaactcgtcg cgcccgcggc ccttcagcac gtcggcgggc
     1201 agctcgtaga gctgattcag cgcgccgccc gggcggatca cgccgttctc gaagtaccct
     1261 tcgcgcttca tccagccgta gttgccgccg ttcctgacga tgttgatctc ttctatatgg
     1321 ttcatgccga tgtcgccgac gaacagcgca ccgtcggcga ggtcccacgc gaggcgatgc
     1381 gcgttgcgga acccgtacgc gtagatctcg ccgagcgtct tcgggtcgcc gtcggcctgg
     1441 agcttgttgt cggccggaat cgtgtaatcg ccgagccctt tcggaccgcc cgtctcgcgc
     1501 gggctgcgcg ggtcgatgcg caggatcgcc gtgatcagcg aatccaagcg ctgcgtcgcg
     1561 ctcggattgc tcgcgttcgg cccgccgccg ttgctgaagc ccaagtcgct gccgctcgtg
     1621 tagatgaggc cgtagtccgg cgagccgggc cgtgccgtcg ggttgaactc gaccgcgcca
     1681 aggggatggc tcgagaactg cacgacgtgc gcgtagcgca ggagctcgcg gcgcgtacct
     1741 tcaaacgtgc tcgccttcgg gtcggtcgcg tgccactcgg tgaggacgtt gtggtacgtg
     1801 acgtcgttcg gcccgaagcc gggcgggatg aagtccggtc gcgcgggatt tccgttcgcg
     1861 ctctcgaggt gcacgctgta ccagagcccg ttgcgctcga attcggggtg gaacgtgaag
     1921 ccgatgaagc cgctttcgag ccgcccgtag aacgcgcgcg ggaacgccgc cgcgacgtcg
     1981 gcgtacaacg acgtgcgcgc gccgtcgacg atataaagga agccgcgcga gtcgttcgcg
     2041 aaccgccggc cgtcgggcgc gtcgcgcacg aagctgacgc gcgcgtagcc cgtcggagtg
     2101 acgtcctgct cgacgggccg gaggtttcgc gtctccggca aacgaacgac gtcgcgcacc
     2161 tcgacggcga ggccgcgctt ctcgatcggc gcgcgcagcg ggttcgaagt gatctgcgct
     2221 cgagccgtta cggcggccaa cagtaacacg gtcaagccgg tccacgatct cacgtcaagc
     2281 atgctccccc caaaggtgcc cccaaaggtg ccggtgcccc caaaggtgcc ggtgttacgg
     2341 tttcggctca accgaagtgc cggtgttacg gctgaaccga agtgccggag ttacggctga
     2401 accgaaccgt aacaccggca ccttcttagc tcaaagttcc tcgccttcga gcgtgcgttg
     2461 ctcggccgtc acgccgaagc ggccgtcgtc gccgcgcacc ggcagcgcgg ccgcgcattc
     2521 gaggtcgtgc gtgaagaaca agcgcacgcg ccgcgagatc ttgtcctcga ggaagccctg
     2581 cttctcgtcg atcaggagct cggggtagcg gtcgtacccc atcgtcacgg gcaaatgcac
     2641 ccacgggcgc cccggaatca ggtcggagca gaacgcgacg ccgccctcgc cgccgatctc
     2701 ggcgagcgcg aggccgggcg tgtggccgtc gctgaagtcg aagcgcacgg cggggccgag
     2761 cagcgtcgac tgcgggcggt cgaggatctc gagccgtccg ctcttgcgca gcagcgccgg
     2821 cagatccgcg atgtacgaag cgcggtcgcg cggatgcggc gcggtcgcac gcgcccacgc
     2881 cgccttgctc acgatgtagc gcgccctcgg aaatagcagc tcgggcgggc gcgactcctc
     2941 gtacgccgaa agcaaaccgc ccgcgtgatc gaagtgcaga tggctcagca cgacggcgtc
     3001 gacgtcctgg tgccgcacgc cgcgcgcttc gagcgagcgc agcagcatgt gctcgctctc
     3061 gtgcacgccg taccgcgcgc gcagcttcgg ctcgaagaat gcgccgatgc ccgtctcgaa
     3121 cagcaccgtc ttgccgccca aatcctgcac gagcaggcag cggcacgcga actcgaggcg
     3181 gttctcgccg tccggcgtga gccacttggc ccagaggttg cgcggcacgt tgccgaacat
     3241 ggcaccgccg tcgaggcgct ggcgattgcc ctcaacggac cagagccgca cgggctttct
     3301 cctcgggctc cggctggccg gccgcgccga actgcagctg cgcgagccgc gcgtagagcg
     3361 ggctcgccga ggtgagctgc gcgtgcgtgc cgctcgcgac gacgcggccc gcgtccatca
     3421 cgacgatgcg atccgccttc tgcaccgtcg cgagccggtg cgcgatgatg atcgtcgtgc
     3481 ggtgcgccat cagcttctcg agcgcttgct gcacgagcag ctcgctcgcc gcatcgagcg
     3541 agctcgtcgc ctcgtcgagc agcaggatcg gcgggtcctt gaggatcgcg cgcgcgatcg
     3601 cgatgcgctg gcgctggccg cccgagagcc ggaggccccg ctcgccgagg aacgtgtcgt
     3661 agccctgcgg cagggcgcgg atgaactcgt cggcctcggc cgcgcgcgcg gcggcctcga
     3721 tgtcggcgtc gctggcattg ggccggccgt agcgaatgtt ctcgcgcgcc gaggcgccga
     3781 acagcatcgt gtcctgcggc ac
//
LOCUS       DASSNN010000379         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1451713, whole genome shotgun sequence.
ACCESSION   DASSNN010000379 DASSNN010000000
VERSION     DASSNN010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451713"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFL84_06990"
     CDS             <1..190
                     /locus_tag="VFL84_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015210142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET7608405.1"
                     /translation="ASNLPLSAYNVSGEYSAVKAAAANGWLDEARIVRENLLAIRRAG
                     ADQIITYHARDALKGKWL"
     gene            190..1518
                     /gene="hemL"
                     /locus_tag="VFL84_06995"
     CDS             190..1518
                     /gene="hemL"
                     /locus_tag="VFL84_06995"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET7608406.1"
                     /translation="MFGRFDAGKGKRQQSEAWFERAKKVTPGGVNSPVRAFGSVGGTP
                     LVIRSALGAMITDSDGREYLDFVGSWGPLILGHAHAKIVAAVESACRRGTSYGAPCQP
                     EIELAEEVVALYPAIEQVRFVSSGTEAVMSAVRLARGATGRDLVVKFSGCYHGHVDYL
                     LVAAGSGLATFGTPSSQGVPKELAALTRVLPLDDAEAAEKLFAAEGDRIAAVIIEPVP
                     ANNGLLLQRPEFLKTLRALCTKHGALLIFDEVISGFRLGSGGAAAHYAIKPDLATFGK
                     VIGGGMPVGAFGGPRKLMKHLSPEGGVYQAGTLSGNPVAMTAGLTTLRTLQKEDGWKR
                     LEALGAYLEQALTSALAEAPLDAKLVRLGSMFWIAWNTREAPRSAEALDANAAKVYAR
                     VFHSLLDGGIALAPSAFEIAFLSLAHTRRDIDRLAEGLRVALASLEAETT"
     gene            1538..2497
                     /locus_tag="VFL84_07000"
     CDS             1538..2497
                     /locus_tag="VFL84_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7608407.1"
                     /translation="MAVRRDPTHLMQIGFLALLSISVAQVGWWMYDGVRYARSVEQRM
                     PNDSAVADDSEARVNRVLWEGGFFLVVLIGGMAVLTRTLRHDAELRRRQQNFLAAVSH
                     EFKSPLASIQLAAETLVLRSRDDDGKRLGRRILEDGERLLRMIDNLLDTTRLEEGRQR
                     LTPQLTNLASAAATAVAAIADRAHASGVAVTRAVPDDLVLNVDPVVVETGLRNLLDNA
                     LKSCVAAKSGSIRVEAARDAGGIVLAVSDDGLGFPPEDAAMIFEKFHRLGDELRRATP
                     GTGLGLYIVKRLVELSGGTVHAQSLGPGRGATVSMRWPGGAAA"
BASE COUNT          393 a          869 c          905 g          387 t
ORIGIN      
        1 agcgagcaac ttgccgctct cggcttacaa cgtctcgggc gaatattccg cggtcaaagc
       61 ggccgccgcg aacggctggc tcgacgaagc gcgcatcgtg cgcgagaacc tgctcgcgat
      121 tcgccgcgcc ggtgccgatc agatcatcac gtatcacgca cgcgacgcgc tgaaggggaa
      181 gtggctctga tgttcggcag attcgacgcc ggcaaaggca agaggcaaca gagcgaggcg
      241 tggttcgagc gcgccaagaa agtcacgccg ggcggcgtga actcgccggt gcgcgcgttc
      301 ggcagcgtcg gcggcacgcc gctcgtgatc cgttccgcgc tcggcgccat gatcacggat
      361 tccgacggcc gcgagtatct cgacttcgtc ggctcgtggg gcccgctgat cttgggccac
      421 gcgcacgcga agatcgtcgc ggccgtcgag agcgcgtgcc ggcgcggcac gtcgtacggc
      481 gcgccgtgcc agcccgagat cgagctcgcg gaagaggtcg tcgcgctcta tcccgccatc
      541 gagcaggttc ggttcgtgtc gtcgggcacg gaggcggtca tgagcgccgt ccggctcgcg
      601 cgcggcgcga cgggacgcga tctcgtcgtg aagttctcgg gctgctatca cggccacgtc
      661 gactatttgc tcgtcgccgc gggctcgggg ctcgcgacgt tcggcactcc gtcctcgcaa
      721 ggcgtgccga aggagcttgc cgcgctgacc cgtgtgctgc cgctcgacga cgccgaagct
      781 gcggagaagc tgttcgcggc cgaaggcgac cgcatcgccg ccgtcatcat cgagcccgtg
      841 ccggcgaaca acggcctgct gttgcagcgg ccggaattct tgaagacgct gcgcgcgctg
      901 tgcacgaagc acggcgcgct cctgatcttc gacgaggtca tcagcggctt ccgcttgggc
      961 tccggcggag ccgcggcgca ctacgcgatc aagccggacc tcgcgacgtt cggcaaagtc
     1021 atcggcggcg gcatgcccgt cggcgcgttc ggcggcccac gcaagctcat gaagcacctg
     1081 tcgcccgaag gcggcgtcta tcaggccggc acgctgtcag gcaaccccgt cgcgatgacg
     1141 gcggggctca cgacgctgcg cacgctgcaa aaagaagacg gctggaagcg actcgaagcg
     1201 ctcggcgcct atctcgagca ggcgctgacg agcgcgctcg cagaagcgcc gctcgacgcg
     1261 aagctcgtcc gcttgggctc gatgttctgg atcgcgtgga acacgcgcga agcgccgcgc
     1321 tccgcggagg cgctcgacgc caacgccgcc aaagtctatg cgcgcgtctt tcattcgctg
     1381 ctcgacggcg gcatcgcact cgcgccgtcg gcgttcgaga tcgcattcct gtcgctcgcg
     1441 cacacgcgcc gcgacatcga ccggctcgcc gaaggcttgc gggtcgcgct cgcgagcttg
     1501 gaagccgaga ctacgtgagg aacgcggagc taagcccatg gcggtgagac gcgacccgac
     1561 tcatttgatg cagatcggct tcctggcgtt gctgtcgatc tcggtcgcgc aggtcggctg
     1621 gtggatgtac gacggcgtgc gctacgcgcg cagcgtcgag cagcgcatgc cgaacgattc
     1681 ggccgtcgcc gacgacagcg aagcgcgcgt caaccgcgtg ctctgggagg gcggattctt
     1741 cctggtcgtg ctgatcggcg gcatggccgt gctgacgcga acgctgcggc atgacgcgga
     1801 gctgcgccgc cgccagcaga atttcctggc cgcggtctcg cacgagttca agagcccgct
     1861 cgcgagcatc cagctcgccg ccgagacgct cgtgctgcgc tcacgcgacg acgatggcaa
     1921 gcggctcggc cggcgcatcc tcgaggacgg cgagcgcctc ctgcgcatga tcgacaacct
     1981 gctcgacacg acgcggctcg aggaaggcag gcagcgcttg acgccgcagc tcacgaatct
     2041 cgcgtcggcg gcggcgacgg ccgtcgcggc gatcgccgac cgcgcgcacg cgagcggcgt
     2101 cgcggtgacg cgtgccgtgc ccgacgatct cgtgctgaac gtcgaccccg tcgtcgtcga
     2161 gaccggcctg cgtaacctgc tcgacaacgc gctgaagtcg tgcgtcgcgg cgaagagcgg
     2221 ctcgatccgc gtggaagcgg cgcgcgacgc cggcggcatc gtgctcgcgg tcagcgacga
     2281 cggcttgggc ttcccgcccg aggatgccgc gatgatcttc gagaagtttc accggctcgg
     2341 cgacgagctg cggcgcgcga cgccggggac cgggctcggc ctctacatcg tgaagcgcct
     2401 ggtcgagctc tcgggcggca ccgtgcatgc gcagagctta ggacccggcc gcggcgcgac
     2461 ggtgtcgatg cgttggcccg gcggagccgc ggcatgaaga cgccggcgcg catcctcgtc
     2521 gtcgacgacg aggcgcatct cgcggcgggg atcc
//
LOCUS       DASSNN010000380         3715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_930415, whole genome shotgun sequence.
ACCESSION   DASSNN010000380 DASSNN010000000
VERSION     DASSNN010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930415"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VFL84_07005"
     CDS             complement(<1..637)
                     /locus_tag="VFL84_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608408.1"
                     /translation="MRTVFPFLCALASVAMSALARAEELLVPQGTVVFGALDERITSN
                     ANKFRVGYPVDGHVWKDVVVDGHTIIRAGTPIELRISRLAGKSVGGQSGALEIMAVAV
                     KAVDGTEISLDGGYDQSATDISGLTRALSMFIWPASFLPGKNAVLDVGTVFDASIPAN
                     TRIVLPDGALPTLKLAPRPDLSVDVIYDDIDQKEGTLPLSLRLCDKEYTREA"
     gene            complement(740..1045)
                     /locus_tag="VFL84_07010"
     CDS             complement(740..1045)
                     /locus_tag="VFL84_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608409.1"
                     /translation="MARRTSSARKPRPNTTQLRAAIRSGRTPTIDSAHEPAAASFDTD
                     DEAAGRPPQAAAIEQALAHERRVERRPARSKSDVVAPLLIAAALLAIAVVAWILLRW"
     gene            complement(1047..1487)
                     /locus_tag="VFL84_07015"
     CDS             complement(1047..1487)
                     /locus_tag="VFL84_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HET7608410.1"
                     /translation="MSVRTFALIFGLLFAATGIAGFIPGLVTPHDAVEHQLAIEQGAG
                     NLLGLFPTNVLHNIVHLAFGVWGLAVFRSTAASIGYARSVAIVYAVLVVMGLIPGLDT
                     VMGLVPLHGNDVWLHAGIAAVAAYFGFVRHAEVEHVSRPAPRGT"
     gene            2298..>3715
                     /locus_tag="VFL84_07020"
     CDS             2298..>3715
                     /locus_tag="VFL84_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608411.1"
                     /translation="MNVQPGSLSARSIHPLDVHDLQQLEDVGFMTCMTLVLLGNYAQT
                     GHFGGPLAYTPFNVVTHLAGRERGGLHFDYRRPKHPYSDKFMLAAGHCAPTCYALWMI
                     MGEALNRKYKATGDRRYYVDPAVAMLPIDALGFRRGAGALATLLKDNGLADHPKFAQA
                     KGRGIRALSGHIESTDVTNDVNGGPSGVGLATAAGKAAFWDFLGAGVDSPKIVAFEGE
                     FALCAGHAQELKTQAMAVQAGKRLRVFFSNNNAGIDDSLLGGVIPSKLADGYVLREQW
                     TSYGWNVFDLADGHDYRQILGVLKAMEDGDPADRRPMIVIGKTTKGYWPAQVAKQIVG
                     YQSHPYGFKMNAEYIVGLAKTFEARYGVKFEGMSAGPVTDMRERLLQFKTNIDVAMSV
                     LDKNGLGDWLADRLVEIGDRVVDKLKLRVDVTRDPFLDERLKVANLPAEPQELSIKNP
                     TSGASKTVKLALFRKAGEAAGAR"
BASE COUNT          680 a         1242 c         1214 g          579 t
ORIGIN      
        1 gcgcctcgcg cgtgtactcc ttgtcgcaga gccgcagcga cagcggcagc gtgccctctt
       61 tctgatcgat gtcatcgtag atcacgtcga cactcaagtc gggccgcggg gccagcttca
      121 aagtcggcaa cgcgccgtcc ggcagcacga tgcgcgtgtt ggccggaatc gacgcgtcga
      181 acacggtgcc gacgtcgagc acggcgttct tgcccggcaa gaagctcgcg ggccagatga
      241 acatcgacag agcccgcgtc aagccggaga tgtcggttgc ggattggtcg taaccgccgt
      301 cgagcgagat ctccgtgccg tccacggcct tcacggcgac ggccatgatc tcgagcgctc
      361 cgctctggcc gcccacgctc ttgccggcga gccgcgagat gcgcagctcg atcggcgtgc
      421 cggcgcggat gatcgtgtgg ccgtccacga cgacgtcttt ccagacgtgg ccgtcgacgg
      481 gatagccgac gcggaatttg ttggcgttcg aggtaatgcg ctcgtcgagc gcgccgaaca
      541 cgaccgtgcc ttgcgggacg agcaactcct cggcacgcgc aagcgccgac atggccacgc
      601 tcgcgagggc gcagaggaac gggaaaacgg ttcgcatcga tataccccgc ggtccgtctt
      661 actcgcggga tcatacggca agcggacgcg gccagtcgat ccggcgcgct gcccgacggc
      721 tcgagaaggc cggctgcgcc taccagcgga gaaggatcca cgcgacgacg gcaattgcca
      781 gcagcgcagc cgcgatcaga agcggcgcca ctacgtccga cttggagcgg gccggccggc
      841 gctcgacgcg ccgctcgtgc gcgagcgcct gctcgattgc cgcggcctgc ggggggcggc
      901 cggccgcttc gtcgtccgtg tcgaacgacg cagcggccgg ctcgtgggcg gagtcgatcg
      961 tcggcgtgcg cccgctccgg atcgccgcac gcagctgcgt cgtgttcggc cgcggcttgc
     1021 gcgccgaaga agtacgccgc gccacgtcag gtcccccgcg gagcgggccg gctcacgtgc
     1081 tcgacctcgg cgtggcgcac gaagccgaag tacgccgcga ccgcggcgat gccggcgtgc
     1141 agccaaacgt cgttgccgtg cagcggcacg aggcccatca ccgtgtcgag ccccggtatc
     1201 aggcccatga ccacgagcac ggcgtacacg attgcgacgc tgcgcgcgta accgatcgac
     1261 gcggcggtgc tgcgaaatac cgcgaggccc cagacgccga acgcgaggtg cacgatgttg
     1321 tgcagcacgt tcgtcggaaa caggccgagc aagttgccgg cgccctgctc gatcgcgagt
     1381 tgatgctcga ccgcatcgtg cggcgtgacg aggccgggaa tgaatccggc gatgcccgta
     1441 gcggcgaata gcagcccgaa aatcaatgca aacgttcgta cgctcatagg tgccctcctt
     1501 ggcagcgagc tgacgaggct gcgctagctg caagccgcat gccactgaag agtcgagcaa
     1561 tcagactgaa cgaagctcgc actttttgcg cttctctcca acatttgcca ggtcgcgtgc
     1621 ctgcgccgcg cgccgcgact ggcggcatgc aattgctcgc gaacgcgatc ccagaggaga
     1681 gaccgtgttc ggacgacatc tcatgcacgc attcttcggc gttgcggcgg cgctcggctt
     1741 ttcgctgtgc gtttccggcg cgtccgatga gccgctttca agggaacgtc gccgaaattc
     1801 ggcttggcca gatggcggcg cagcggactc aaaacgatgc cgtacgcaag ctcggcgaga
     1861 cgttacggca agaccattcc gtcgcgttgc agcgcgcgaa cagaatcgcc gaatcgatgc
     1921 ccgtcgaggc gccggccgag ccatcggccg gctcgaaacg caagcacgat tcgctcgcga
     1981 agctgtcggg caggaaattt cgacgccgcc tttgcacaac aaatggtcgc cgaccacgag
     2041 gcatcgatcg cgacatatcg cgcccacatg cagagcggcg acgatgcgat tgccagctag
     2101 gtcaaggaat cgctgcccga gctcgaggcc catctcgcaa tggcccggga gcttaaggag
     2161 caatagacgg aaacgacgct ggcccatcgg aatcgaatca tcgacctgta ggcgcccggc
     2221 cggctcgacc gggcttcgga aatcctcact tcggctcgtt cgcgacgcgg cgagccgact
     2281 gtgtagaatt cgagcccatg aacgtacaac ccggctctct ttcggctcgc tccatccacc
     2341 cgctcgacgt ccacgacctc caacaactcg aagacgtcgg cttcatgacc tgcatgacgc
     2401 tggtgctgct cggcaactac gcgcagaccg gccacttcgg cggacccttg gcgtacacgc
     2461 cattcaacgt cgtcactcac ctcgccggcc gcgaaagagg cggcttgcac ttcgactatc
     2521 ggcgcccgaa gcacccgtat agcgacaagt tcatgctggc cgcgggccat tgcgctccga
     2581 cctgctatgc gctgtggatg atcatgggcg aagccctgaa tcgaaagtac aaggccacgg
     2641 gcgaccgtcg ctattacgtc gatcccgccg tcgcgatgct gccgatcgac gcgctcggct
     2701 tccggcgcgg cgccggcgcg ctcgcgacgc tgctcaaaga caacgggctg gccgatcatc
     2761 cgaaattcgc acaggcgaag ggacgcggca tccgcgcgct atccggccac atcgagtcga
     2821 ccgacgtcac gaacgacgtc aacggcggcc cctcgggcgt cggcctcgcc acggcggccg
     2881 gcaaggccgc cttctgggac ttcctcgggg ccggcgtcga ctcgccgaag atcgtcgcct
     2941 tcgaaggcga gttcgcgcta tgcgcgggcc acgcgcagga attgaagacg caggcgatgg
     3001 ccgtgcaggc cggcaagcgg ctccgcgttt tcttctcgaa caacaacgcc ggcattgacg
     3061 actcgctgct cgggggcgtg atcccgagca agctcgccga cggctacgtg ctgcgcgagc
     3121 agtggacgtc gtacggctgg aacgtcttcg atcttgcgga cgggcacgac tacaggcaga
     3181 tcctcggcgt gctgaaggcg atggaggacg gagaccccgc ggatcgccgg ccgatgatcg
     3241 tgattggcaa gacgacgaag ggctactggc ccgcgcaagt cgcgaagcag atcgtcggct
     3301 atcagagcca tccttacggc ttcaagatga acgccgagta catcgtcggc ctcgcgaaga
     3361 ctttcgaggc gcgctacggc gtcaagttcg aaggcatgtc cgccgggccg gtcacggaca
     3421 tgcgcgagcg gctgctccag ttcaagacga acatcgacgt cgcgatgtcg gtgctcgaca
     3481 agaacggcct cggcgactgg ctcgcggatc gcctcgtcga gatcggcgac cgcgtcgtcg
     3541 acaagcttaa gctgcgcgtc gacgtcacgc gcgacccgtt tctcgacgag cggctcaagg
     3601 tcgccaacct tcccgcggag ccgcaggagc tcagcatcaa gaaccccacg tcgggagcca
     3661 gcaagaccgt caagctcgcg ttgttccgca aagcgggcga agccgccggc gcgcg
//
LOCUS       DASSNN010000381         4824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1451738, whole genome shotgun sequence.
ACCESSION   DASSNN010000381 DASSNN010000000
VERSION     DASSNN010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451738"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1807
                     /locus_tag="VFL84_07025"
     CDS             281..1807
                     /locus_tag="VFL84_07025"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HET7608412.1"
                     /translation="MSGAVSSSRSMLQVNALLVATVGVGFLNNVAIAWLFGLTRKVDA
                     YYAAMILPGLFMVLFVEYLGKNFLPVFARARSQSFETASELTSAMVTVTALITAVAVA
                     VIVLASKPLFAVLLPGFDAAEVELVSSYLWIMAPASVLGAVSTFHGYLFQHEDKYTQL
                     VAIGAIQPLVNLGAVLALGPFVGEYSLPIAFTGGKLIGFVLLARGAQYRYRPRITLRP
                     EWERKVLVNSAILMGSGLLVRTRGLVGNYLASLLGEGAISALALGYKLVEPLERTTFG
                     GVRMLMYSRTARLAVEENASEMSRLYRLGVAASFLLVAPPLAWMCFESEFLVSFLFER
                     GAFDMSMTKLVALAVIGFAPSVLLAGVNGVLSNAFYALDRVKIPALVMPVGTVAYLAA
                     APFLYKPYGVLGLSLSPSAAYAVVFVLLLYFLNRRLTALCGGELLLRVIGYTALAVAA
                     FGAAKLLSTFVEWPRFVEAVTTLSVGAALYFGVLVALRDRTLLEVFRYFRRAHPRFAR
                     "
     gene            1929..2945
                     /locus_tag="VFL84_07030"
     CDS             1929..2945
                     /locus_tag="VFL84_07030"
                     /inference="COORDINATES: protein motif:HMM:NF025755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HET7608413.1"
                     /translation="MPRQIVDLVAHKLRLNIAPYDYYAFEFYKGGKSWDEKRRYVGRH
                     GSAYWPFASNPLRFNGLFTHKYVEKLLLKGAGLPTAQLLGVVGEDGDIRTREELGEFL
                     ARVTHDVVVKPVSGTHGKRVLVLSRVAGGLTAGGKPCTAEDVWQHVSADRKKGFLVEE
                     KLTNAPEIAALYPSSLNTFRVVTIKTLDKRWCLAAAYLRVGAGGNVVDNVAAGGILVR
                     VDAEGRTCSAHHDGRSIAQHPDTGAQLVGIRLPGYRAACDLALEASRKLGFVGTIGWD
                     IAVTDRGAVIVEGNLWWTPSFQAALGGAITDELAASLPKRTPFERWDKSMMHPKFDQR
                     GRRG"
     gene            complement(2953..4482)
                     /locus_tag="VFL84_07035"
     CDS             complement(2953..4482)
                     /locus_tag="VFL84_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608414.1"
                     /translation="MREFRFHCNPTLPRLAWCAKVAQGAEAVVVEHGSWVETGPNFFA
                     EGSWNRPFDEACLGDAEVVLGSGGTLAGGSAAFTPTTHTMERLYSLRRGPAVLVSNSL
                     PFLLEAARVKLDPKNWSYERQLMTFLRGYAKAAKRLELADGATVSLHYHATFSIDSAL
                     ELGSRPPPAPPSFDSYDRYIAYLSSTLEVLHRNASSPRRKVAYAPLSTISSGYDSPAC
                     AVLAKRLGCRRAVTFTEAREGFNDRPLKTLDDSGEHIAAILGLDVAKFSRSAYLTADD
                     YPEALFIATGGGGDDVVLSALRDTLEGTMLFTGMLGDTLWSTGETQSPSLSEQYRFLY
                     PAGGSLQEFRLRTGFIHVPVPLLTFTRHGDVQRISRSVEMRPWRVGGDYDRPIPRRLV
                     EEAGVPRRAYAKEKRAITQPFWLQKSDSTCMSTRSLRDLEEFRARVTAQYRLGKLRMQ
                     LNERLQRAVGAWRIRAARLTRDPYHSDAYLEAAMADPLRFHWAVEKVSEAYRGCRIGA
                     L"
     gene            complement(4550..>4824)
                     /locus_tag="VFL84_07040"
     CDS             complement(4550..>4824)
                     /locus_tag="VFL84_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608415.1"
                     /translation="RYTLRLLEKATQVAKFKLNALKLEAALLQCYRWINDGYSWAGGR
                     LNLRNALHTWAVAEAVRSLRERGLGQPGTEARPPAPQSRAGAVAAS"
BASE COUNT          748 a         1641 c         1592 g          843 t
ORIGIN      
        1 gcttgagata cgatcgtcgc gcagggtcgc gcttcctcgc gctgcgcgat cgaacgtaaa
       61 ggaatgccgg catccttgcc ctcaccgtta cgttcacgaa gccaccgtca taagcaaaat
      121 ccatgcccct cgcgcccgac cgtcgcgcaa ccgcaacggt cgcgagcgat aatgggccga
      181 gttgttcgac agctcctcga gcccgcgagc ggtgttgaac gagggaggtc accgccttgg
      241 ggtcgacggc accggtccac gtgttggttc caccacgccc atgagcggcg ccgtgtcgag
      301 ctcgcgcagc atgctgcagg tcaacgcgct gctcgtggcg actgtcggcg tgggattcct
      361 gaacaacgtc gcgatcgcgt ggctgttcgg tctcacacgc aaggtcgacg cgtattacgc
      421 cgcgatgatc ctgccggggc tgttcatggt cctgttcgtc gagtacctcg gcaagaactt
      481 cttgcccgtg ttcgcgcgcg cgcgaagtca aagcttcgag acggcgtccg agctgacgtc
      541 ggcgatggtc accgtcacgg cgttgatcac ggcggtcgcg gtcgcggtca tcgtcctcgc
      601 gagcaagccg ctttttgcgg tgctgctgcc cggcttcgac gcggcggagg tcgagctcgt
      661 cagcagctac ctctggatca tggctccggc cagcgtcctc ggcgccgtgt cgacgttcca
      721 cggctatctt tttcagcacg aggacaagta cacgcagctc gtcgcgatcg gcgcgatcca
      781 acccctcgtc aacttgggcg cagtgctcgc cctcggcccg ttcgtcggcg aatactcgct
      841 gccaatcgcc ttcacgggcg gcaagctcat cggcttcgtt ttgctcgcgc gcggcgcgca
      901 gtatcgctat cggccgcgaa tcacactcag gcccgagtgg gaacgcaaag tcctcgtcaa
      961 ctcggcgatc ctgatgggca gcggactgct ggtccgcacc cgcgggctgg tcggcaatta
     1021 cctcgcctcg ctgctcggag aaggcgcgat ctcggcgctc gcgctcggct acaagctcgt
     1081 cgagcctctc gagcgcacga cgttcggcgg tgtgcgcatg ctgatgtaca gccgcaccgc
     1141 gcggctcgcc gtcgaggaga acgcgagcga gatgtcgcgg ctctatcgcc tcggcgtcgc
     1201 ggcgagcttc ctgctcgtcg cgccgccgct cgcctggatg tgcttcgaga gcgagttcct
     1261 cgtctcgttc ttgttcgagc gcggcgcatt cgacatgagc atgacgaagc tcgtcgcgct
     1321 cgccgtgatc ggcttcgccc cctcggtgct gctcgccggc gtcaacggcg tgctgtcgaa
     1381 cgccttctac gcgctcgatc gcgtcaagat tcccgcgctc gtgatgcccg tcggcacggt
     1441 cgcgtatctc gcggccgcgc cgttcctata caagccgtac ggcgtcttgg gcttgtcgct
     1501 gtcgccgagc gccgcgtacg cggtcgtgtt cgtgctgctg ctgtactttt tgaaccggcg
     1561 gcttaccgcg ctgtgcggcg gcgagctgtt gctgcgggtc atcggctaca cggccctggc
     1621 agtcgccgcg ttcggcgccg cgaagctgct ctcgaccttc gtcgagtggc cgcgcttcgt
     1681 cgaggccgtg acgactttga gtgtaggagc cgcgctctac ttcggcgtgc tcgtcgcgct
     1741 tcgcgatcgc acgctgctcg aggttttcag gtacttccgc cgagcacatc cgcggttcgc
     1801 gcgctagccg cagtggcacg cggtttgcct atacctcctt cgagcaatct gcgcaggacg
     1861 accgtgccat gttcgcgagg atcgcatcgc gcctgacccg cattcgcgaa gcggcgagct
     1921 ttcgcagcgt gccgcgtcaa atcgtcgacc tggtcgcgca caagctgcgg ctcaacatcg
     1981 ccccgtacga ctattacgct ttcgagttct acaagggcgg caagagctgg gacgagaaga
     2041 gacgctacgt gggccggcac gggtccgctt attggccgtt cgcgagcaat ccgctgcggt
     2101 tcaacgggct cttcacgcac aaatacgtcg agaagctgct gctcaagggc gcgggactgc
     2161 ccaccgcgca gctgttgggc gtggtcggcg aggacggcga catccgcact cgcgaggagc
     2221 tcggcgaatt cttggcgcga gtcacgcacg acgtcgtcgt gaaaccggtc agcggaacgc
     2281 acggcaagag agtgctcgtg ctgtcccgcg tcgccggcgg cctgacggcg ggcggcaagc
     2341 cctgcacggc ggaagatgtc tggcagcacg tgagcgcgga ccggaaaaaa ggcttcctcg
     2401 tcgaggagaa gctcacgaac gcgccggaga tcgccgcgct ctatccctcg agcctcaaca
     2461 cgtttcgcgt cgtgacgatc aagacgctcg acaagcgttg gtgcctcgcc gccgcctatc
     2521 tgcgcgtcgg cgcgggcggc aacgtcgtcg acaacgtcgc ggcgggcgga atcctcgtcc
     2581 gagtcgacgc cgaggggcgc acctgctcgg ctcatcacga cgggcggtcg atcgcgcaac
     2641 atcccgacac cggcgcgcag ctcgtcggca ttcggctgcc gggttatcgc gccgcctgcg
     2701 atctggcgct cgaagcgagc cgcaagctcg gcttcgtggg cacgatcggc tgggacatcg
     2761 ccgtcacgga ccgcggcgcc gtgatcgtcg aggggaacct ctggtggacg ccgtcgtttc
     2821 aggccgcgct cggcggcgcc atcacggacg agctggccgc gtcgctaccc aaacgcacgc
     2881 cgttcgaacg ctgggacaag tcgatgatgc atccgaagtt cgaccagcgc gggcggcgcg
     2941 gctgacgcgg cttcacagcg cgccgatgcg gcagccgcgg tacgcctcgg agactttttc
     3001 gacggcccaa tggaagcgca gcggatcggc catcgccgcc tcgagataag cgtcggagtg
     3061 atacgggtcg cgcgtcaggc gcgccgctcg aatgcgccac gcgccgacgg cgcgctgcag
     3121 ccgttcgttc aactgcatgc gcaacttgcc gaggcggtat tgcgccgtga cgcgggcgcg
     3181 aaactcctcg agatcgcgca gcgagcgcgt gctcatgcag gtcgaatcgg acttctgcag
     3241 ccagaacggc tgcgtgatcg cgcgtttttc cttcgcgtac gcgcggcgcg gcacgcccgc
     3301 ctcttcgacg aggcgccgcg gtatcggccg atcgtagtcg ccgccgacgc gccacggccg
     3361 catctcgaca gaccgcgaga tgcgctgcac gtcgccgtgg cgcgtgaacg tcaacagcgg
     3421 gaccggcacg tggatgaacc ccgtgcgcag ccggaactcc tgtaggcttc cgccggccgg
     3481 atacaagaac cgatactgct cgctcaacga agggctctgc gtctcgcccg tgctccacaa
     3541 cgtgtcgccg agcatgccgg tgaacagcat cgtgccttcg agcgtgtcgc gcagcgcgct
     3601 caacacgacg tcgtcgccgc cgccgcccgt cgcgatgaac aacgcctcgg ggtagtcgtc
     3661 tgccgtgaga tacgcactgc gcgaaaactt cgcgacgtcg agcccgagaa tcgctgcgat
     3721 gtgctcgccg ctgtcgtcga gcgtcttcag cgggcggtcg ttgaagcctt ccctggcctc
     3781 ggtgaacgtg accgcacggc ggcagcccag ccgcttcgcg agcacggcgc acgccggcga
     3841 atcgtagccc gacgagatcg tcgagagcgg cgcatacgcg accttgcgtc gcggtgagct
     3901 cgcattgcgg tgcaggacct cgagcgtgct cgaaaggtac gcgatgtacc ggtcgtacga
     3961 gtcgaagctc ggcggcgccg gcggcggccg cgacccgagc tcgagagcgg aatcgatcga
     4021 gaacgtggcg tgatagtgca gcgaaaccgt agcgccgtcc gcgagctcga gccgcttcgc
     4081 cgccttcgcg tagccgcgca ggaatgtcat cagctgccgc tcgtaggacc agttcttcgg
     4141 atcgagcttg acgcgcgccg cttcgagcaa aaacggcagc gagttcgaca cgagcacggc
     4201 gggcccgcgc cgcaggctat agagccgctc catcgtatgc gtcgtcggcg tgaacgcggc
     4261 gctgcctccg gcgagcgttc cgcccgaccc gagcacgacc tcggcgtcgc cgagacatgc
     4321 ctcgtcgaac ggccggttcc agctgccttc cgcgaaaaaa ttcgggccgg tctcgaccca
     4381 cgagccgtgc tcgacgacga cggcttcggc gccttgcgcg actttcgcgc accaggcaag
     4441 ccgcggcagc gtcggattgc aatgaaaacg gaactcgcgc attcggcggt gcgtttgggc
     4501 cttacggccg cggccccgtg cgcctcgagc tcgcgcggct cacatctgct tagctcgccg
     4561 cgacggcgcc tgcgcgagac tgaggcgccg gcggccgagc ctctgtgccc ggctggccga
     4621 gcccacgctc gcgcaggctt cgaacggctt ccgcgacggc ccacgtgtgc agcgcgttcc
     4681 gcagattgag gcgcccgccg gcccacgagt agccgtcgtt gatccatcga taacactgca
     4741 gcaacgcggc ctcgagcttc agcgcattca gcttgaactt cgccacctgg gtcgctttct
     4801 cgagcaggcg cagcgtgtag cggt
//
LOCUS       DASSNN010000382         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_671723, whole genome shotgun sequence.
ACCESSION   DASSNN010000382 DASSNN010000000
VERSION     DASSNN010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671723"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="VFL84_07045"
     CDS             <1..177
                     /locus_tag="VFL84_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7608416.1"
                     /translation="ALEKLKPSEREALALRYVADLSHREIAEACGLDEAAARKRVSRA
                     LDRLRSVMPTEEIE"
     gene            201..>2036
                     /locus_tag="VFL84_07050"
     CDS             201..>2036
                     /locus_tag="VFL84_07050"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET7608417.1"
                     /translation="MDDLAALVAGEPDAIARHAEHLASCDACRDARHDATQLADKLAV
                     AGADYVPGGDLAERVLAKLDAQAEPKQAAPIDVKQGPPLEAKQEVPLVAKQEAPIAAK
                     LDAKPAVQSDAKPAETKPAAVRDIATARGKRRLWIVAGAAAALAAGVVGVAMMKRGGD
                     ASTESSVAATGPIGKVKTISRAAAEQGDGIAIKTDTGWRPLRADEMLPRGAELRTDER
                     TRTSLELADGSRIVLDHRTNVIFDAQEPRRIKLVAGRIAADVAHVENRQASITTPSGR
                     IDVVGTRFSVTAADQLTAVQVVRGSVVLTAADGSHDEVRAGEEGLIENGKLSVAATPE
                     LVKETAWSELSQPTAQTDQETTAGLGALRAYKPGEKRDKDWKLALANHEVKVRISGPV
                     ARTEITETFRNDSDTTLEGVYQFPLPPDAQIDSLALDVEKEPGGFIQGAFVDKERAAK
                     IWRGVIDKATPKHIRRDPLIQQEIVWVPGPWKDPALLDWKRGGRFELRIFPIPKHGTR
                     TIKLAYTQVVTPRGPQREYVYPLPHSFDGSTVADKMKVDIEVRGAQQGTVRTVGYDFK
                     ADPARQDVNAMAFEQGGFVPRGDVVVDYRADQGDAELRAWTFQGGA"
BASE COUNT          349 a          709 c          720 g          258 t
ORIGIN      
        1 gcgctcgaga agctcaagcc gagcgagcgt gaagctctcg cccttcgtta tgtcgctgac
       61 ctgagccacc gcgagatcgc ggaggcctgc gggctcgacg aagccgctgc gcgcaagcga
      121 gtcagtcgcg cgctcgaccg gcttcgctcg gtcatgccca ccgaggagat cgaataatgc
      181 ggaccgccca tgatgatgtg atggatgacc tcgccgcgct cgtcgccggc gagcccgacg
      241 cgatcgcgcg tcacgccgag cacctcgcga gctgcgatgc gtgccgcgac gcgcgccacg
      301 atgcgaccca gctcgcggac aagctcgctg ttgccggcgc cgactacgtg cccggcggcg
      361 atctcgccga gcgcgtgctg gcgaagctcg acgcgcaagc cgagccgaag caagcggcac
      421 cgatcgacgt gaagcaaggc ccgccgctcg aggcgaagca agaagtgccg ctcgtcgcga
      481 agcaagaggc gccgatcgcc gcgaagctcg acgcgaagcc cgccgtgcag agcgacgcga
      541 aacccgccga gacaaagccc gcggcggtgc gcgacatcgc gaccgcgcgt ggcaagcgcc
      601 ggctgtggat cgttgccggg gctgctgccg cgctcgcggc tggtgtcgtc ggcgtcgcga
      661 tgatgaagcg cggcggtgat gcctcgacgg agagcagcgt cgccgcgacg ggtccgatcg
      721 gcaaggtcaa gacgatctcg cgcgccgccg ccgagcaggg cgacggcatc gcgatcaaga
      781 ccgataccgg ctggcgcccg ctgcgtgcgg acgagatgct gccgcgcggc gccgagctgc
      841 gcaccgacga gcgcacgcgc acgtcgctcg agctcgccga cggctcgcgc atcgtgctcg
      901 atcaccgcac gaacgtgatc ttcgacgcgc aggagccgcg ccggatcaag ctcgtcgccg
      961 gccgcatcgc cgccgacgtc gctcatgtcg agaaccggca ggcatcgatc acgacgccga
     1021 gcggccgcat cgacgtcgtc ggcacgcggt tctcggtcac cgccgccgac cagctcacgg
     1081 ccgtccaggt cgtgcgcggc agcgtcgtgc tgaccgccgc cgatggcagc cacgacgagg
     1141 tgcgcgccgg cgaggagggc ctgatcgaga acggcaagct gtcggtcgcg gcgaccccgg
     1201 agctcgtcaa ggagaccgcg tggtccgagc tgtcgcagcc gaccgcgcag accgatcagg
     1261 agaccaccgc cggcctcggc gcgctgcgcg cgtacaagcc gggcgagaag cgcgacaagg
     1321 actggaagct cgcgctcgcc aatcacgagg tcaaggtgcg catctcgggg ccggtcgcgc
     1381 gcaccgagat caccgagacg ttccgcaacg acagcgacac cacgctcgag ggcgtctatc
     1441 agttcccgct gccgcccgac gcacagatcg acagtctcgc gctcgacgtg gagaaggagc
     1501 ccggtgggtt catccagggc gcgttcgtcg acaaggagcg cgcggcgaag atctggcgcg
     1561 gcgtgatcga caaggcgacg ccgaagcaca tccgccgcga tccgctgatc cagcaggaga
     1621 tcgtgtgggt gcccggtccg tggaaggatc cggcgctcct cgactggaag cgcggcggcc
     1681 gcttcgagct gcgcatcttc ccgatcccga agcacggcac gcgcacgatc aagctcgcgt
     1741 acacgcaggt cgtgacgccg cgcgggccgc agcgcgagta cgtctatccg ctgccgcatt
     1801 cgttcgacgg ctcgacggtc gccgacaaga tgaaggtcga catcgaagtg cgtggtgcgc
     1861 agcagggcac cgttcgcacc gtcggctacg acttcaaggc ggatccggcg cgccaggacg
     1921 tcaacgcgat ggcgttcgag cagggcggct tcgtgccgcg cggcgacgtc gtcgtcgatt
     1981 accgcgccga ccaaggtgat gccgagctcc gcgcgtggac attccagggc ggcgct
//
LOCUS       DASSNN010000383         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1303128, whole genome shotgun sequence.
ACCESSION   DASSNN010000383 DASSNN010000000
VERSION     DASSNN010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303128"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VFL84_07055"
     CDS             complement(<1..989)
                     /locus_tag="VFL84_07055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7608418.1"
                     /translation="MRILVVAPQPFYQERGTPIATRLLLEALQAAGHSVDVLTYHVGE
                     DPKLPGVHVFRAPAVPFVRDVPIGFSLRKLLCDVALLWRLFTLTRRSRYDVLHAVEEA
                     VFLSLLVRAFAGRRSTLGSDKLDRLGCRVVYDMDSSLPEQLLGKYAALRFVDGVLRRL
                     ERLAIAQSDLVLAVCNDLAIRARSYATGTPIDVVEDVSLLGSGGAKGETENLRRGLLY
                     GELLVLYVGNLEHYQGVDLMLDAIAKLESPPMKFVAVGGNHEAVVAYRRRVAELGLGG
                     QVAFIGARPLAQLGPLLEQADVLVSPRLQGQNTPMKLYSYLAAGKAVLATRIRS"
     gene            complement(995..1720)
                     /locus_tag="VFL84_07060"
     CDS             complement(995..1720)
                     /locus_tag="VFL84_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7608419.1"
                     /translation="MLHAITKETPDIETASESYARRFAGAAGRYLLSEQEAAIRAVVP
                     DLRVGNVLDVGGGHTQLIPLLRSLARDVVVFGSDVRSLERVRRDFPDCATATGDLLAL
                     PFESRSFDVVVAVRLLPHVRNWPRLLSELCRVARSAIVVDYPRRTGFNGLTPLLFPLK
                     LRLEGNTRHYRNFRDAELDEVLQVCGFEPRERRAQFLLPMVVHRRASGFAPLRGLERA
                     AKALRLTQALGSPVILRADRRRA"
     gene            complement(1734..>2482)
                     /locus_tag="VFL84_07065"
     CDS             complement(1734..>2482)
                     /locus_tag="VFL84_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608420.1"
                     /translation="HRAALGRDAAAGFALLALAGPLVGNALFSSPFELFRYNAAFNTL
                     FFVFVALAFVHWRELVPAWRPTLFLEPRKTGAALGTAMLVLLATAYDLNPLRGWLAVQ
                     RAYSNQGVIYRTFGITAYSDFKTTADYVARHAKPHDLIVTPDSREYYNYLGRVDLWLR
                     SGRYEDQSYVQHGTRRDLYVDTPLVATLQELKAALSRPNQTKWVLASSRVLADPKAPV
                     APEIRAYLRSQEQHVVYVGLDRDRKVYRFE"
BASE COUNT          437 a          815 c          880 g          350 t
ORIGIN      
        1 gagcggatgc gcgtcgcgag caccgccttg ccggccgcga gatacgagta caacttcatc
       61 ggcgtgttct gcccctgcag ccgcggcgag accagcacgt cggcttgctc gagcagcggg
      121 ccgagctgcg cgagaggccg cgccccgatg aacgcgactt gtccgccgag gccgagctcg
      181 gcgacacgcc tgcggtaggc gacgacggct tcgtggttgc cgcctaccgc gacgaacttc
      241 atcggcggcg attcgagctt ggcgatcgcg tcgagcatga gatcgacgcc ctggtaatgc
      301 tcgaggttgc cgacgtacag cacgagcagc tcgccgtaca gcaggccgcg gcgcaggttc
      361 tcggtctcgc ccttcgcgcc gccgctgccg agcagcgaga cgtcctcgac gacgtcgatc
      421 ggggtgccgg tcgcgtaact gcgtgcgcgt atcgcgaggt cgttacagac ggcgagcacc
      481 agatccgatt gcgcaatcgc gagccgctcg agccgccgca gcacgccgtc gacgaagcgc
      541 aaggccgcgt acttgccgag caattgctcc ggcagcgacg aatccatgtc gtacacgacg
      601 cggcagccga ggcggtcgag cttgtcgctg ccgagcgtgc tccgtcggcc ggcaaaggcg
      661 cggacgagca agctcaggaa gacggcttcc tcgaccgcgt gcagcacgtc gtagcgagag
      721 cggcgcgtca acgtgaagag ccgccagaga agcgcgacgt cgcagagcag cttgcgcagc
      781 gagaatccga tcgggacgtc gcgcacgaac ggcacggcgg gcgcgcggaa cacgtgcaca
      841 ccgggcagct tcggatcctc gccgacgtgg tacgtcagca cgtcgacgct gtgcccggcc
      901 gcctgcagcg cttcgagcag caaccgtgtc gcgatcggag tgccgcgctc ctgatagaac
      961 ggctgcggcg cgacgacgag gatacgcatg cgcgtcacgc tcgccgccga tcggcgcgca
     1021 ggatgacggg cgagcccaaa gcctgggtca ggcgcagcgc tttcgcggcg cgctcgagcc
     1081 cgcgcagcgg cgcgaacccg ctcgccctgc ggtgcacgac catcggcagc aggaattgcg
     1141 cccggcgttc gcgcggctcg aagccgcaca cttgcagcac ctcgtcgagc tccgcgtcgc
     1201 ggaaattgcg gtagtgccgc gtatttccct ccagccgcag cttgagcggg aacaacagcg
     1261 gcgtgaggcc gttgaagccc gtcctgcgcg gataatcgac gacgatcgcg gaccgggcca
     1321 cgcggcagag ctcggacagg aggcgcggcc aattccgcac gtgcggcagc agccgcaccg
     1381 ccacgacgac gtcgaacgag cggctctcga acggcagggc gagcagatcg ccggtcgcgg
     1441 tcgcgcagtc cggaaagtcg cggcgcacgc gctcgagact gcggacgtcg ctgccgaaga
     1501 cgacgacgtc gcgcgcgagg ctgcgaagca gcggaatcag ctgcgtgtgg ccgccgccga
     1561 cgtcgagaac gttgccgacg cgaagatcgg gaaccaccgc gcgaatcgcg gcttcctgct
     1621 cggaaagcaa atagcggccg gcggcgccgg cgaaccgccg cgcgtacgac tcggaggcgg
     1681 tttcgatgtc gggggtttct ttcgtgatgg cgtgcagcaa gcgcgttcaa tcctcattcg
     1741 aagcgataga ctttgcggtc gcgatcgagg ccgacgtaga cgacgtgctg ctcctgagag
     1801 cgcagatagg cccgaatctc gggcgcgacg ggcgccttcg gatcggccaa taccctgctc
     1861 gacgcgagca cccatttggt ttggttcggg cgcgagagcg ccgctttcag ctcctgcagc
     1921 gtcgcgacga gcggcgtgtc gacgtacaga tcgcgccgcg tcccgtgttg cacgtagctc
     1981 tgatcctcgt agcggccgct ccggagccag aggtcgacgc gtcccaaata gttgtagtac
     2041 tcgcgcgagt ccggagtgac gatgaggtcg tgcggcttcg cgtgccgtgc tacgtagtcg
     2101 gccgtggttt tgaaatcgct gtaggcggtg atgccgaacg tccgatagat cacgccttgg
     2161 ttgctgtacg cgcgctggac ggcgagccag ccgcgcaacg ggttcaagtc gtacgccgtc
     2221 gcgagcagga cgagcatcgc agtgccgagc gccgcccccg tcttgcgcgg ctcgagaaac
     2281 agcgtcggcc gccaggccgg cacgagctcg cgccagtgaa cgaaggcgag cgcgacgaac
     2341 acgaagaaca gcgtgttgaa ggccgcgttg taccggaaca gctcgaaggg cgacgagaac
     2401 aacgcgttgc cgacaagcgg tcccgcgagc gcgaggaggg cgaacccggc ggcggcgtcg
     2461 cggccgagcg cggcacgatg ga
//
LOCUS       DASSNN010000384         4283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1451886, whole genome shotgun sequence.
ACCESSION   DASSNN010000384 DASSNN010000000
VERSION     DASSNN010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451886"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFL84_07070"
     CDS             <1..600
                     /locus_tag="VFL84_07070"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HET7608421.1"
                     /translation="ERDAVTAEAAVVGSSLDAASRDGNRSTRYLVTYRFKPSNGAALE
                     QTEEIPLELWETLAAGDEVTVRYLVDDPGTARMRSPTPPWVPPLVAALTAAFAALGVL
                     IARPGWRRVLVLVRVRRNGVAAPATVTEVAPAGLVINRIPQWRLKYEFRDARGERHEG
                     ESGYLRPHEAAEWQAGDRGTVLYDSGRAADNVWLGKDAR"
     gene            complement(611..1546)
                     /locus_tag="VFL84_07075"
     CDS             complement(611..1546)
                     /locus_tag="VFL84_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7608422.1"
                     /translation="MKRNNALLLVALLVPLAWLPSVAAQLKIYGNTTTIELAPVLLAA
                     QELYGDTTAVRNGGIPDLFDEDAADVATNAETQALRQSVDHADLRTILTVSEGWYRIV
                     ARRSAGISRLADLRGKRITTAPNTSSAYYLHKMLGTVGMTTADVTVVPITPLTGMPRA
                     LANRDVDAVTIWEPEIENAAAAIGSDAIELQDRRVYRELFGLHTTAAKLADPAKRREI
                     VAFVRAVVRASERIRTRPQEVWPLVAERTGFDRALVEKVWHHESYPGALVPDLLDVMV
                     EEDPFVARERNRAPRTRAQLAVLIDASVLREALAE"
     gene            complement(1563..2855)
                     /locus_tag="VFL84_07080"
     CDS             complement(1563..2855)
                     /locus_tag="VFL84_07080"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HET7608423.1"
                     /translation="MSALGETLATALVVSGLAACCLALLPRTPPRIRFAVAVFGLAAW
                     LVPWGWIRMALPSSSAAAPLADWLGTAQISAALSATERGQIYFPPKIDLSPFALVSPF
                     ALGAAVAAAFVVGFVLFVRDCVALRRCLRVWRARSRPGEHLRSLLPRELASIAADIRI
                     VEGSAIAAASGALRPTIWLGGRHTQSQLEVALVHEAWHVRARDPVWVAMIAAVRRAFW
                     WNPLVAYLARQALLMIESACDHRCAAHLGKPHYMSQLASLLLGGTAPATRLVAAVGAA
                     NLDVQRLRLLGQPLRVRARDVALVAALAVSAAAAATAAVVEREAAPPAAPRSEAGSLP
                     ATPAGTALAALLRAAKSGDSALLDELLGAYTPQEVPLPLPHASRDARVVDILRSEPLR
                     IEYVVESRESGPRYVGEIAVSDAASARITDLKTNLLAW"
     gene            complement(2852..3232)
                     /locus_tag="VFL84_07085"
     CDS             complement(2852..3232)
                     /locus_tag="VFL84_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007623738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HET7608424.1"
                     /translation="MSIRLSEFELDVMTHFWRDRELSAPELYERIGAERGVAYSTVKT
                     IVDRLEQKGAIERVANRGRTIVYSPTIKRERVRKPLVKAFLRRFFGDDLRPLFAQLLQ
                     DEKLSAEELEYLRKLVAETERSKK"
     gene            complement(3286..3834)
                     /locus_tag="VFL84_07090"
     CDS             complement(3286..3834)
                     /locus_tag="VFL84_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET7608425.1"
                     /translation="MLIALNKPYGVVSKFSPEPGKRTLADYVPIRGVYPAGRLDTDSE
                     GLLLLTDDGALQARIANPRHKLPKTYWAQVEGAPTDEALAALREGVDLGDFVTKPAGA
                     RRIDEPAGLWPRDPPIRYRAKIPTAWLELTLREGKNRQVRRMTARVGFPTLRLVRAAI
                     GRLGVAGLAPGEWREVDVENLS"
     gene            complement(3842..>4283)
                     /locus_tag="VFL84_07095"
     CDS             complement(3842..>4283)
                     /locus_tag="VFL84_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608426.1"
                     /translation="AYLMYGFPTETVQETVDALERVRQLFEAGCIQSAYWHRFSATAH
                     SPIGLRPEQYGITLKPPPNITFAHNDVAFDDPVGADHDFLGAGLRKALYNYMLGVGLD
                     ADVRAWFEPAQSVRRRPGRRQRRRAAAVPKTTVPRDLIERSLAE"
BASE COUNT          638 a         1457 c         1541 g          647 t
ORIGIN      
        1 gagcgcgacg ccgtgacggc ggaagcggcc gtcgtcggct cgtcgctcga cgcggcgagc
       61 cgcgacggca atcggagcac acggtatctc gtgacctacc ggttcaagcc gtcgaacggc
      121 gccgcgctcg agcagaccga ggagattccg ctcgagttgt gggagacgct tgcggccggc
      181 gacgaagtaa cggtgcgcta tctcgtggac gatcccggca cggcacgcat gcgctcgccg
      241 acgccgccgt gggtgccgcc gctcgtcgcc gcgttgacgg cggctttcgc cgcgctcggc
      301 gtgctcattg cgaggccggg ctggcggcgc gtgctggtgc tggtgcgcgt ccggcgcaac
      361 ggcgtggcgg cgcccgcgac cgtcaccgag gtcgcgcccg cgggcctcgt catcaatcgc
      421 atcccgcagt ggcggctcaa gtacgagttc cgcgatgcgc gcggcgaacg ccacgaaggc
      481 gagagcggct acctgcggcc gcacgaggcg gcggagtggc aggcggggga tcgcggcacc
      541 gttctttatg actccggccg agccgccgat aacgtgtggc tcggcaagga cgcgcggtaa
      601 ggccgctctc tcactcggcc agcgcctcgc gcagcacgct cgcgtcgatg aggacggcga
      661 gctgcgcgcg cgtgcgcggc gcgcggttgc gttcccgtgc gacgaacgga tcttcctcga
      721 ccatgacgtc gagcaagtcc ggcacgagcg cgccgggata gctttcgtga tgccagacct
      781 tctcgacgag cgcgcggtcg aagcccgtgc gctcggcgac gagcggccag acttcttgcg
      841 gccgcgtgcg aatgcgctcg ctcgcgcgga ccacggcacg cacgaacgcg acgatctcgc
      901 gccgcttcgc gggatcggcg agcttcgcgg ccgtcgtgtg caagccgaat agctcgcgat
      961 agacgcggcg gtcctggagc tcgatcgcgt cgctgccgat cgctgcggcc gcgttctcga
     1021 tctcgggctc ccagatcgtg accgcgtcga cgtctcggtt tgcgagcgcg cgcggcatgc
     1081 cggtcagcgg cgtgatcggc acgaccgtga cgtccgcggt cgtcatgccg acggtgccga
     1141 gcatcttgtg caggtagtac gccgaggagg tattcggcgc agtagtgatg cgcttgccgc
     1201 gcaggtccgc gagccgcgag atgcccgccg agcggcgcgc gacgatgcga taccagcctt
     1261 ccgagaccgt caagatcgtg cgcagatctg cgtgatcgac ggattgccgc agcgcctgcg
     1321 tctcggcgtt cgtcgcgacg tcggcggcgt cctcgtcgaa cagatccgga attccgccgt
     1381 tgcgcacggc cgtcgtgtcg ccgtacagct cttgcgccgc cagcagcacg ggcgcgagct
     1441 cgatcgtcgt cgtgttgccg tagatcttga gctgcgcagc cacactcggc agccatgcga
     1501 gcgggacgag caatgcgacg agcaacagcg cgttgttcct tttcatgcgg ttcgttcgcg
     1561 ccttaccagg cgagcaggtt cgtcttcaga tccgtgattc tcgcggacgc cgcgtccgag
     1621 accgcgatct cgccgacata ccgcggcccg ctctcgcggc tttcgacgac gtactcgatt
     1681 cggagcggct cgctgcgcag gatgtcgacg acgcgcgcgt cgcgcgaggc gtgcggcagc
     1741 ggcagcggca cctcttgcgg cgtgtaagcg ccgagcagct cgtcgagcag cgccgagtcg
     1801 cccgacttcg cggcgcgcag cagcgcggcc agcgctgtgc cggccggcgt ggccggcagg
     1861 ctgcccgctt cggaccgcgg cgcggcgggc ggcgccgctt cgcgctcgac gacagcggcc
     1921 gttgcggcgg cggccgccga cacggcaagc gccgcgacga gcgccacgtc gcgcgctcgc
     1981 acgcgaagcg gctggccgag caaccgcagc cgttgcacgt cgaggttcgc tgcgccgacc
     2041 gccgcgacga ggcgtgttgc gggcgccgtc ccgccgagca gcaacgacgc gagctgcgac
     2101 atgtaatgag gcttgccgag gtgcgcggcg cagcggtggt cgcaggccga ctcgatcatg
     2161 agcaacgctt gccgcgcgag gtatgcgacg agcgggttcc accagaaggc gcgccgcacg
     2221 gccgcgatca tcgcgaccca gaccggatcg cgcgcgcgca cgtgccacgc ctcgtgaacg
     2281 agcgctacct cgagctggga ttgcgtgtgc ctgcccccga gccagatcgt tggccgcagc
     2341 gcgccggacg ccgccgcgat ggcgctgccc tcgacgattc tgatgtccgc ggcgatggaa
     2401 gcgagctcgc gaggcaggag cgagcgcaag tgctcgccgg gccggctgcg cgcgcgccag
     2461 acccgcaggc agcgccgcag cgcgacgcag tcgcgcacga atagcacgaa gccgacgacg
     2521 aacgccgccg cgacggccgc gccgagcgca aaaggggaca cgagcgcaaa aggggacaga
     2581 tctattttcg gaggaaaata gatctgtccc ctttcagttg ccgagagcgc agcggagatt
     2641 tgcgcggtgc cgagccagtc ggcgagcggc gcggccgcgc tcgaggacgg cagcgccatg
     2701 cgaatccagc cccacggaac gagccacgcc gctaaaccga aaaccgctac ggcgaagcgg
     2761 atgcgcggcg gcgtgcgcgg cagtaacgcg aggcagcacg cggcgagacc gctcacgacg
     2821 agcgccgtcg ccagcgtctc gccgagcgcg ctcacttctt gctccgttcc gtttcggcga
     2881 cgagcttgcg caggtactcg agctcttcgg cgctcaactt ctcgtcttgc agcagctgcg
     2941 cgaacagcgg gcgcaggtcg tcgccgaaga agcggcgcag gaacgctttg acgagcggct
     3001 tacggacgcg ctcgcgcttg atcgtcgggg aatagacgat ggtgcggcct cggtttgcga
     3061 cgcgctcgat cgcgcctttc tgctcgagcc ggtcgacgat cgtcttgacg gtcgaatagg
     3121 cgacgccgcg ctccgcgccg atgcgctcgt agagctcggg ggccgagagc tcccggtcgc
     3181 gccagaaatg cgtcattacg tcgagctcga actcggacag tctaatggac atggcggcga
     3241 ccgtggagac ttatatctac gagtgtagtg atgccgcagt gaccgtcaac tcaagttttc
     3301 tacgtcgact tcgcgccatt cgccgggcgc caagcccgcg acgccgaggc ggccgatcgc
     3361 ggcgcggacg agccgcagtg tcgggaagcc gacgcgcgcc gtcatgcgcc ggacctgccg
     3421 gttcttgcct tcgcgcaacg tgagctcgag ccaggcggtc gggatcttcg cgcgatagcg
     3481 aatcggcggg tcgcgcggcc acagtccggc cggctcgtcg atgcgccttg cgccggccgg
     3541 cttcgtcacg aaatcgccga ggtcgacgcc ctcgcgcagc gcggcgagcg cttcgtcggt
     3601 cggcgcgcct tcgacttgcg cccagtaggt cttcggcagc ttgtggcgcg gattcgcgat
     3661 gcgcgcctgg agcgcgccgt cgtcggtcaa gagcagcaag ccttcgctgt ccgtgtcgag
     3721 ccggccggcg gggtagacgc cgcggatcgg cacgtagtcg gcgagcgtgc gcttgcccgg
     3781 ctccgggctg aacttcgaga cgacgccgta cggcttgttc agcgcgatca gcacggcgcc
     3841 gtcattccgc gaggctgcgc tcgatcaggt cccgcggcac ggtcgtcttc ggcaccgctg
     3901 cggcgcggcg ccgttgccgg cggcccggcc tgcgccgcac ggattgcgct ggctcgaacc
     3961 aagcgcgcac gtcggcgtcg agcccgacgc ccagcatgta gttgtagagc gccttgcgaa
     4021 gcccggcgcc gaggaagtcg tggtcggcgc cgacgggatc gtcgaacgcg acgtcgttgt
     4081 gcgcgaacgt gatgttcggc ggcggcttga gcgtgatgcc gtattgttcc gggcgcaagc
     4141 cgatcggcga atgcgccgtc gcgctgaacc ggtgccagta cgcggactgg atgcagcccg
     4201 cctcgaacaa ctgccgcacg cgctcgagcg cgtcgacggt ttcctgcacc gtctcggtgg
     4261 ggaagccgta catcaaatac gcg
//
LOCUS       DASSNN010000385         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1451916, whole genome shotgun sequence.
ACCESSION   DASSNN010000385 DASSNN010000000
VERSION     DASSNN010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451916"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1650)
                     /locus_tag="VFL84_07100"
     CDS             complement(199..1650)
                     /locus_tag="VFL84_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET7608427.1"
                     /translation="MSATTMDDAAAAETARRNKLVINLILVSSFVVILNETMMSNATP
                     PLMQSLGISAAAAQWVTTAFLLTMAVGIPMTGFLLQRLHTRVVYILAMSLFSAGTFAA
                     LIAPDLTLLVVARVVQALGTSVMMPLLMTTIMQLSTPDTRGKTMGYVSTVISVAPAIG
                     PVLSGVILDYLHWRWLFVLVLPIALFALWLGWRRIENVTTPRAAHLDIASVILSAFAF
                     GGIVYGISSFGVPPAARTMPPLVPFAIGGVAMVAFVWRQLVLQRTDSALLDLRVFRAG
                     TFRVSLGVFGLLMLTLFGTIILLPMYLQRVLELTPMQTGLLLLPGGLLMGRLGPFVGR
                     RYDKVGPRPLVIPGVIIVTAVLWAMTQLGTQTPWPYILGGHMIMCVGFALLFTPLFTT
                     CMASVPPQLYSHGSAVLGSFNQVGGAAGVALFIALMSAQEAKLAAGGSLPLEALAGGI
                     RLAFLVGAVGALLAVASTFFVRKPDAPAAPAAH"
     gene            1803..2678
                     /locus_tag="VFL84_07105"
     CDS             1803..2678
                     /locus_tag="VFL84_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7608428.1"
                     /translation="MADQTRRDFIATSAAAVAAAAAAGRAVGQTAASAAAPPGAFYQK
                     GDVRIRYVDTGGSGFPLLVIPGGGLNSTIDGLIRSHPFNPLDELKNEYRVVTADLRNA
                     YAGASTGPLEADRPWDAYTDDHLGVMDHLGIDRFMVLGFCIGGPFIWNLIERAPSRVV
                     AAVTAQPSGFRPEQPTLFYDNNTMTWGPELMKRRPEITKEMVDRFLTRMYKTNADFVF
                     TVTRDFVRQCQTPVLVLPDDIPAHPYAVAMETVMLAPKSEVSIYPWKEPPERIPVAVR
                     QIRSFLRAHRPAAHG"
BASE COUNT          556 a          953 c          966 g          445 t
ORIGIN      
        1 catgttcgga gccggcgctt gcgcgaacgc gacgccgatg ccgaccgcca aaaccgcaag
       61 tccgccgagt gccgttagac gtttcatttg ttactcccct ctgtggaacg aattgcgaaa
      121 cccatcgaaa ctagcgaagc gccggagcga aatcaattca gggttcttga gcttcggcct
      181 cggctccatc cttatatatc aatgagcggc gggagccgcc ggcgcgtcgg gcttgcgcac
      241 gaagaacgtg gaagcgacgg cgagcaatgc gccgacggcg ccgacgagga acgcaaggcg
      301 gatgccgccc gcgagcgctt cgagcggaag gctaccgccg gccgcgagct tcgcttcttg
      361 cgccgacatg agcgcgatga acagcgctac gcccgccgca ccgccgactt ggttgaacga
      421 gcccaagacg gcgctgccgt gcgagtagag ctgcggcggc accgacgcca tgcacgtcgt
      481 gaacagcggc gtgaacagca gcgcgaagcc cacgcacatg atcatgtggc cgcccaaaat
      541 atagggccat ggcgtttgcg tgccgagctg cgtcatggcc cagagcacgg ccgtcactat
      601 gatgacgccg gggatcacga gcgggcgcgg accgactttg tcgtagcgcc tgcccacgaa
      661 cggcccgagc cggcccatca gcaatccacc gggcaagagc aacaagcccg tttgcatcgg
      721 cgtcagctcg agcacccgct gcagatacat cggcagcagg atgatcgtgc cgaacaacgt
      781 cagcatcagc agcccgaaca cgcccagcga aacccggaac gtgcccgcgc gaaacacgcg
      841 cagatcgagc aatgcggaat ccgtgcgttg cagcacgagt tgccgccaca cgaacgcgac
      901 catcgcgact ccgccgatcg cgaacggaac caacggcggc atcgtgcgag ccgcgggcgg
      961 cacgccgaac gagctgatcc cgtagacgat tccgccgaac gcaaacgcgg agaggatcac
     1021 cgacgcgatg tcgaggtgcg ccgcgcgcgg cgtcgtcacg ttctcgatgc gccgccagcc
     1081 gagccacaaa gcgaacagcg cgatcggcag cacgagcacg aacagccagc gccagtgcag
     1141 gtaatccaag atcacaccgg acagcaccgg cccgatggcg ggcgccacgg aaatgacggt
     1201 cgagacatag cccatcgtct tgccgcgcgt gtcgggcgtc gacagctgca tgatcgtcgt
     1261 catgagcagc ggcatcatga cggacgtgcc gagcgcttgc acgacgcgcg cgacgacgag
     1321 cagcgtaagg tccggtgcaa tcagcgcagc gaacgtgccg gccgagaaca gcgacatcgc
     1381 gaggatgtaa acgacgcgcg tgtgcaggcg ctggagcagg aagcccgtca tcggaatgcc
     1441 gacggccatc gtcagcagga acgcggtcgt gacccattgc gcggccgcgg cggaaatgcc
     1501 gaggctctgc atcagcggcg gtgtcgcgtt gctcatcatc gtctcgttca agatgacgac
     1561 gaacgacgac accaggatca gattgatgac gagcttgttg cggcgcgcgg tctcggccgc
     1621 tgcagcgtcg tccatagtcg tagcgctcat ggtttcgctg aagccgcaac gcggcgagat
     1681 gctctcggcc ttggggaggc gcgcattgtg cgcctcgcgg ctcgcggccg ccagcttgac
     1741 gggacgccgc gttcggacga gcctaaagca aaaggcgggc ctaacaccgc gtggggggaa
     1801 ccgtggcgga tcagacgaga cgcgatttca ttgcgacgag cgccgctgcg gttgcggcgg
     1861 cggcagcggc gggccgcgcg gtcggccaaa ccgcggcgag cgcggccgcg ccgccgggcg
     1921 ctttctatca gaaaggcgac gtgcgcattc gctatgtcga cacgggcggc tccggttttc
     1981 cgctgctcgt gatcccgggc ggcggcttga actcgacgat cgacggattg atccgctcgc
     2041 acccgttcaa cccgctcgac gagctcaaga acgagtaccg cgtcgtcacg gcggacttgc
     2101 gcaacgctta tgccggagcg tcgacggggc ctctcgaagc tgaccggcct tgggatgcgt
     2161 ataccgacga tcacctcggc gtcatggatc accttggcat cgaccggttc atggtgctcg
     2221 gcttctgcat cggcggcccg ttcatctgga acttgatcga gcgcgcgccg agccgcgtcg
     2281 tcgcggcggt gacggcgcaa ccgagcggct tccggccgga gcagccgacg ctcttctacg
     2341 acaacaacac gatgacgtgg ggccccgagc tcatgaaacg ccggcccgag atcacgaagg
     2401 aaatggtcga tcgattcctg acgcggatgt acaagaccaa tgccgacttc gtgttcacgg
     2461 tgacgcgcga cttcgtgcgc cagtgccaga cgccggtgct cgtgctgccc gacgacatcc
     2521 cggcgcatcc gtacgccgtc gcgatggaga ccgtgatgct ggcgccgaag tccgaggtca
     2581 gcatctatcc gtggaaagag ccgccggagc gaattccggt cgccgtgcgg cagatccgct
     2641 cgttcctgcg cgcgcaccgg cccgccgcgc acggctagcg aatttcgcta aaggttggcg
     2701 tgtccggcgc acggcctgcc cgtgcccgct cgtgccgcct ccataagtac ctgattctgc
     2761 gagcccttgc cttggcacga gtttggcaat gctctgcgcg cgcgggcgcg cgggcccgct
     2821 gacggaggga gctccatcgt gaaagcgctc ggaaccgtgg tgactgtgct ggtgatcatc
     2881 atcggcgccc tcgtgttgat tcccatcctg ggggtcatgt
//
LOCUS       DASSNN010000386         4225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_572048, whole genome shotgun sequence.
ACCESSION   DASSNN010000386 DASSNN010000000
VERSION     DASSNN010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572048"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VFL84_07110"
     CDS             <1..1207
                     /locus_tag="VFL84_07110"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET7608429.1"
                     /translation="SGILALAGGCANESAPPEPSPAGRPLTLDTHIDIPLDYATATAD
                     PLDADLKVNLTKMAAGGLDVGFFIVYVDQTARTSEGYEKARTEAETKLAAIHRMAEQL
                     YPERIAIAYSAADVRRIAASGKLVAAIGVENGYSLGPNLERLERFYEQGARYMGLVHD
                     GDNDLARSARPSAALGDAADSTAGVSALGAEAIARMNRLGVMVDVSHGSKQTALDAMR
                     LSAAPIIASHSGVAAINAHPRNMDDETLLALKANGGVVQVVAFDGYLKRQPPEKAAAQ
                     RALRESVGLTANMRVSSLPPERRAAYEAGLRDIEAQWPAATVADLVDHIDYAVKLIGV
                     DHVGIASDFDGGGGITGWNDAAETANVTAALAARGYSRDDIEKIWSGNLLRVWSEVER
                     EAAELKTAP"
     gene            complement(1337..2347)
                     /locus_tag="VFL84_07115"
     CDS             complement(1337..2347)
                     /locus_tag="VFL84_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HET7608430.1"
                     /translation="MSAQLQAKDFASSQEVRWCPGCGDYAVLKSVTKALADVGAVPEK
                     TVFVSGIGCSSRFPYYVETYGFHTIHGRAPAIATGLKLANPELDVWVVTGDGDGLSIG
                     GNHLLHALRRNVGFKILLFNNEIYGLTKGQYSPTSRLGTRSPSTPAGSFDTPVVPGAF
                     ALGAGAKFIARAIDVDKAGLGGVLEAAHGFEGAAFVEIFQNCIVYNDGVFNSFTERDK
                     AADAQIRVEHGRPLLFGEGGSKGLSFDSKTLQLKVVDAKANPNDVLVHDETNRTLAQL
                     LLTMPQPELPVALGVILRQPGVAFEKAYYANQPTKLKRTGKVADALRQTSIWRVPAAN
                     AK"
     gene            complement(2344..4218)
                     /locus_tag="VFL84_07120"
     CDS             complement(2344..4218)
                     /locus_tag="VFL84_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HET7608431.1"
                     /translation="MDSQSVVVRFAGDSGDGVQLIGTQFVAATALAGNDFATFPDYPA
                     EIRAPAGTTFGVSAYQIHLGARPITTAGDAPHVLVAFNPAALKVNLPLIADGALIILN
                     IDSFTSRGLAKAGYEKDPRQSGLLDGYQVIEVDITKHTLEATEKFGLSKSDAGRCKNF
                     WALGLVQWMFNHDIASIEEYLNKKFAKDTTIRDANLAALHAGHAYGETAELAAAVPHV
                     GAVTAQFPPGEYRGVRGGEALALGLAAAGELADKPIIFCGYPITPASALLHQLARYGH
                     MGVGTFQAEDEIAAVCAAIGASYGGAIGVTSSSGPGIALKTEAIGLAINAELPLVVVN
                     SQRGGPSTGLPTKTEQSDLYQAVYGRNADAPVPVIAARSPSDCFEVAIEAVRIAVRHM
                     TPVILLTDGYLANAAEPWRIPNMDEIPRIAVNAPGERAAGQDLPSFIFKRDPETLGRP
                     WVAPGMPGLMYRVGGIEKDIHTGNISYDAQNHQAMSELRAAKVASVAKFIPPQKVEVG
                     PEKGALAVVGWGSTYGALYQAVRAMPAKERVSHIHIRYLSPLPENLGELLRGFDQILV
                     PEMNMGQLATLLRDKLGVETIQFNKVTGQPFLIGELVKKIRTVLGAKPAVRAVRGERA
                     "
BASE COUNT          679 a         1439 c         1433 g          674 t
ORIGIN      
        1 gtcaggcatc ctcgcgctgg ccggcggctg cgcgaacgaa tcggcgccgc ccgagccctc
       61 gcccgccggc cgcccgctca cgctcgacac gcacatcgac atcccgctcg actacgcgac
      121 ggccacggcc gacccgctag acgcggacct caaggtcaat ctcacgaaga tggcggccgg
      181 cggcctcgac gtcggcttct tcatcgtcta cgtcgaccag accgcgcgca cgagcgaggg
      241 ttacgagaag gcgagaaccg aggcggagac gaagctcgcc gcgattcatc gcatggccga
      301 gcagctctat ccggagcgca tcgcgatcgc gtactccgcc gcggacgttc ggcgcattgc
      361 ggcgtcgggc aagctcgtcg cggcgatcgg cgtcgagaac ggctactcgc tcgggccgaa
      421 cctcgagcgg ctcgagcgct tctacgagca gggcgcgcgc tacatgggcc tcgtgcacga
      481 cggcgacaac gacctcgcgc gctcggcgcg cccgagcgcc gcgctcggcg acgccgcgga
      541 ttcgacggcc ggcgtcagcg cgctcggcgc cgaagcgatc gcgcgcatga accgcctcgg
      601 cgtcatggtc gacgtgtcgc acggctcgaa gcagacggcg ctcgacgcga tgcgcctgtc
      661 cgcggcgccc atcatcgcgt cgcactcggg cgttgcggcc atcaacgcgc atccgcgcaa
      721 catggacgac gagacgttgc tcgcgctgaa agcgaacggc ggcgtcgtgc aagtcgtcgc
      781 gttcgacggc tacctgaagc ggcagccgcc cgagaaggcc gccgcgcagc gcgcgctgcg
      841 cgaaagcgtc ggcctgaccg caaacatgcg cgtaagctcg ctgccgccgg agcggcgcgc
      901 cgcgtacgag gccgggttac gcgacatcga agcgcagtgg ccggccgcga cggtcgcgga
      961 tctcgtcgat cacatcgact acgccgtcaa gctgatcggc gtcgaccacg tcgggatcgc
     1021 gtccgacttc gacggcggcg gcggcatcac gggctggaac gacgcggccg aaacggcgaa
     1081 cgtgacggcg gcgctcgccg cgcgcggcta cagccgcgac gacatcgaga agatctggag
     1141 cggcaacctg ctgcgcgtct ggagcgaagt cgaacgcgaa gccgcggagc tcaaaaccgc
     1201 gccttgatct gcaaagggac aagctgtaaa ggggacagat ctattttcct ttctgtaaag
     1261 gggacagatc tattttcctt tcggaaaata gatctgtccc catttcgagg gtccccattt
     1321 cgaggcgcgt tcgaggctac ttcgcgttcg cggccggcac gcgccagatg ctcgtctggc
     1381 gcagcgcatc ggcgactttg cccgtgcgct tcagcttcgt cggctgattc gcgtagtacg
     1441 ccttctcgaa ggcgacgccg ggctggcgca ggatcacgcc gagcgcgacc gggagctccg
     1501 gctgcggcat cgtcagcagc agctgcgcga gcgtgcgatt ggtctcgtcg tgcacgagca
     1561 cgtcgttcgg attcgccttc gcgtcgacga ccttcagctg aagcgtcttg gaatcgaacg
     1621 agagcccctt cgagccgccc tcgccgaaga ggagcggtcg cccatgctcg acgcgaatct
     1681 gcgcgtcggc ggccttgtcg cgctccgtga acgagttgaa cacgccgtcg ttgtacacga
     1741 tgcagttctg gaagatctcg acgaacgcgg cgccctcgaa gccgtgcgcc gcttcgagca
     1801 cgccgcccaa gcccgccttg tcgacgtcga tcgcgcgcgc gatgaacttc gccccggcgc
     1861 cgagcgcgaa cgcgcccgga acgaccggcg tatcgaacga gcccgcgggc gtcgacggcg
     1921 agcgtgtgcc gagccgcgac gtcggcgagt actggccctt cgtgaggccg tagatctcgt
     1981 tgttgaacag caggatcttg aacccgacgt tgcggcgcag cgcgtgcagc agatgattgc
     2041 cgccgatcga caagccgtcg ccgtcgccgg tcacgaccca gacgtcgagc tccggattcg
     2101 cgagcttgag ccccgtcgcg atcgccggcg cgcggccatg gatcgtgtgg aagccgtacg
     2161 tctcgacgta gtacggaaag cgcgacgagc agccgatgcc ggagacgaac acggtcttct
     2221 cgggcaccgc gccgacgtcg gcgagcgcct tcgtcacgga cttgagcacg gcgtagtcgc
     2281 cgcagcccgg gcaccagcgc acctcttgcg acgaagcgaa gtccttggct tgcaactgcg
     2341 cgctcatgct cgctctccac gcaccgcgcg cacggcgggt ttcgcgccga gcaccgtgcg
     2401 gatcttcttg acgagctcgc cgatcaggaa cggttggccc gtgaccttgt tgaactgaat
     2461 cgtctcgacg ccgagcttgt cgcgcagcag cgtcgcgagc tggcccatgt tcatctcggg
     2521 aacgaggatc tggtcgaagc cgcgcagcag ctcgcccaag ttctcgggga gcgggctcag
     2581 gtagcgaatg tgaatgtggc tcacgcgctc cttcgcgggc atcgcgcgca ccgcctggta
     2641 cagcgcgccg tacgtcgagc cccagccgac cacggcgagc gcgcccttct cgggccctac
     2701 ctcgaccttc tgcggcggaa tgaacttcgc gaccgatgcg actttcgccg cgcgcagctc
     2761 cgacatcgct tggtggttct gcgcgtcgta ggagatgttg ccggtgtgga tgtccttctc
     2821 gatgccgccg acgcggtaca tgagacccgg catgccgggc gcgacccacg gccggcccaa
     2881 cgtctcgggg tcgcgcttga agatgaagct cggcaagtct tggccggcgg cacgctcgcc
     2941 cggcgcgttg acggcgatgc gcgggatctc gtccatgttc gggatgcgcc acggctcggc
     3001 ggcgttcgcg agatagccgt cggtcagcag gatcacgggc gtcatgtgcc gcacggcgat
     3061 gcgcacggct tcgatcgcga cctcgaagca gtcgctcggg ctgcgcgcgg caatgacggg
     3121 gaccggcgcg tcggcgttgc ggccgtacac ggcctggtac aggtcggatt gctcggtctt
     3181 cgtcggcaag cccgtcgagg gtccgccacg ctgcgagttg acgacgacga gcggcagctc
     3241 cgcgttgatc gcgaggccga tcgcctccgt cttcaaggcg atgccggggc ccgagctcga
     3301 cgtgacgccg atcgcgccgc cgtacgacgc gccgatcgcg gcgcagacgg cggcgatctc
     3361 gtcctcggcc tggaacgtgc cgacgcccat gtgcccgtag cgcgcaagct ggtgaagcaa
     3421 cgccgacgcc ggcgtgatcg gatacccgca aaaaatgatc ggcttgtcgg ccagctcgcc
     3481 ggccgcggcc aagccgagcg cgagcgcctc gccgccgcgc acgccgcgat actcgcccgg
     3541 cgggaattgc gcggtgactg cgcccacgtg cggaacggcc gccgcgagct cggccgtctc
     3601 gccataggcg tgaccggcgt gcagcgcggc caagttcgcg tcgcggatcg tcgtgtcctt
     3661 cgcgaacttc ttgttcaagt actcttcgat cgacgcgatg tcgtgattga acatccactg
     3721 cacgaggccg agcgcccaga aattcttgca gcgcccggcg tcgctcttgc tcaagccgaa
     3781 tttttcggtc gcctcgagcg tgtgcttcgt gatgtcgacc tcgatgacct gatagccgtc
     3841 gagcagcccg ctctggcgcg ggtccttctc gtagccggcc ttcgcgagcc cgcggctcgt
     3901 gaagctgtcg atgtttagaa tgatcaaggc gccgtccgcg atcagcggca agttgacctt
     3961 gagcgcggcg ggattgaacg cgacgagcac gtgcggcgcg tcgccggccg tcgtgatcgg
     4021 ccgcgcgccg aggtgaatct gatacgcgga cacgccgaac gtcgtgcccg cgggcgcgcg
     4081 gatctcggcg gggtagtccg ggaacgtcgc gaagtcgttg ccggcgagcg ccgtcgccgc
     4141 gacgaattgc gtgccgatca gctgaacgcc gtcgccggag tcgccggcga accgaaccac
     4201 tactgattgc gaatccatgc tgtta
//
LOCUS       DASSNN010000387         2790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_809986, whole genome shotgun sequence.
ACCESSION   DASSNN010000387 DASSNN010000000
VERSION     DASSNN010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809986"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFL84_07125"
     CDS             <1..273
                     /locus_tag="VFL84_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7608432.1"
                     /translation="GAVSLARAFHPQIAVLDIGMPGLDGYAVARALRREPKGEDMLLI
                     ALTGWGQKEDKRLAAAAGFDAHIVKPADPERLVQLMLAQREDLSCL"
     gene            complement(635..1261)
                     /locus_tag="VFL84_07130"
     CDS             complement(635..1261)
                     /locus_tag="VFL84_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6151 family protein"
                     /protein_id="HET7608433.1"
                     /translation="MNDTAKLSCRCGKVSGRVIDASPQAASRIVCYCDDCQAFLHHLG
                     RADLLDAHGGTDVVQVAPASVAFDRGAEQIVGLRLTPKGLYRFYAACCNTPLGNTLSP
                     AIPFVGIVAQAFEDADRSFGKPVGAILGKFAVGTPPAGSTRMNLPLLASAVRKVLGWR
                     LRGKAWPHPFFDRETRKPIRPITVLTNDEREALRPLCGPRDAPPESSL"
     gene            1353..2621
                     /gene="cls"
                     /locus_tag="VFL84_07135"
     CDS             1353..2621
                     /gene="cls"
                     /locus_tag="VFL84_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET7608434.1"
                     /translation="MSTLIWAILSSAATALVCLLGVVLTRARGTPYLDLDPDSYPPIQ
                     DALPMMAGLTDSSVREGNSVNVFQNGALFPAMLADIANAEQTVHLETFVWHSGGVERT
                     FVDALAAKAQAGVKVRVLIDSLGGSSASKAELARLKESGAKLANYCKPRWWNLGRFNH
                     RTHRKLLVIDGRIGYTFGHGVADEWLGQAEDPDHWRDTGVRLEGPVVHALQSVFAQNW
                     IEETHTLLAGDNCFPVLERRGDVSMHVVSSASGDAVSSVATLYTVAIASARHEVLIQN
                     PYFAPGDGVVELLAMMVRRGVAVHLMVPGKHTDSPFVRRAGCSLYTPMLEAGVRLYEF
                     EPTLLHQKIVVVDGLWSHVGSTNFDARSLALNEEVGVGILDAGVAAELKAAFQQDLRR
                     SHELKLEEWRRRPIYDRVFERCAYLLHEQL"
BASE COUNT          499 a          861 c          908 g          522 t
ORIGIN      
        1 ggcgcggtct cccttgcgcg agcctttcat ccgcagatag cggtgctcga tatcgggatg
       61 ccgggtctcg acgggtacgc tgtcgcgcgt gcgctgcgcc gcgagccgaa gggtgaagac
      121 atgctgctca ttgcgctgac cggctggggc cagaaggaag acaagcgcct tgccgcggcc
      181 gcaggcttcg acgcccacat cgtgaagccg gcggacccgg aacggctagt gcagctcatg
      241 cttgctcagc gcgaagatct atcctgcctg taagggtgtc ctccgctgat aatgggactg
      301 cgagctcact ccagcgtccc ggaatcggga cgcctagcgc gtcaggagac atgcgaggtg
      361 gagatccgac tttctacccg gtcggctcat tcgagagttg cggacatcgc cgaccacgcc
      421 gatgatcgtg agccacggtg aataggcttc tccgattttg agtcgctcgc cgatagtgtt
      481 tcgccgcccc atcaaaacga ctcggcggat ctcgcacgct gctggcgaag ccggccgcgg
      541 ctggcgctga cgctcccgcg ggagcgtttc tgtaatgaga atcgtttagg aaatccctcc
      601 gcaggcgcgc gcggacctcc gcgaatggct cggcctatag ggacgattcc ggcggagcgt
      661 ctcgcggacc acacaatggc cgcagcgctt cccgctcgtc gttggtaaga accgttatcg
      721 gccgaatcgg cttgcgggtc tcacgatcga agaacgggtg cggccaagcc ttgccgcgca
      781 agcgccagcc gaggaccttg cgcacggcgc tagcgagcag gggcagattc attcgtgtcg
      841 agcccgcggg cggggtgccg acggcgaact ttccgagaat cgctccgacg ggtttgccga
      901 aagaacggtc ggcgtcctcg aacgcctggg cgacgatgcc gacgaaggga atcgcggggc
      961 tcagcgtgtt gccgagcggc gtgttgcaac acgcggcata gaagcgatag aggcccttcg
     1021 gcgtgagccg caacccgacg atttgctcgg ccccgcggtc gaacgcgacg cttgccggcg
     1081 ccacctgaac gacgtcggtt ccgccgtgcg cgtcgagcag atccgcccga ccgaggtggt
     1141 gcaaaaacgc ctggcagtcg tcgcagtagc agacgatgcg gctcgccgcc tgcggcgatg
     1201 cgtcgatgac gcgcccgctg accttgccgc aacgacagct tagcttagca gtatcgttca
     1261 tcggtcaaac tcctcagagt cgctctaact gctggcgacg cggctcgccg gcaaataacc
     1321 ttcgagtcac atcgtttgtc aaagagagcc tgatgtctac gctcatctgg gccattctgt
     1381 cctccgccgc caccgccttg gtgtgtctgc tcggcgttgt gctcacgcgc gcacggggca
     1441 caccgtacct cgatctcgat ccggattcgt atcccccgat ccaggatgcc ttgccgatga
     1501 tggctgggct taccgatagc tccgttcgcg aaggcaacag cgtcaacgta tttcaaaacg
     1561 gcgccttgtt ccccgcaatg ctcgccgaca tcgcgaatgc cgagcaaacg gtccatctcg
     1621 agacgttcgt ttggcacagc ggcggggtcg agaggacgtt cgtcgacgcg ctagcggcca
     1681 aggcgcaggc gggcgtgaag gtgcgagtgc tcatcgactc gctcggcggg tcgagcgcga
     1741 gcaaggctga gcttgcgcgc ctcaaggaga gcggggccaa gctcgcaaac tactgcaagc
     1801 cgcgctggtg gaacctggga cgcttcaatc accggacgca tcgcaagctg ctcgtgatcg
     1861 acggccgcat cggctacacg ttcggccatg gcgtagcaga cgagtggctc ggccaggcag
     1921 aggacccgga tcattggcgg gataccggag tgcgtctcga ggggcccgtc gtgcacgcgt
     1981 tgcaatccgt gttcgcccag aattggatcg aggagacgca cacgctgctc gcgggcgata
     2041 attgcttccc ggtgctcgag cgccgcggcg atgtgtcgat gcacgttgtg agcagcgcgt
     2101 cgggcgacgc cgtgtcgtcg gtcgctacgc tctacacggt ggccatcgcc tcggcgcgac
     2161 acgaagtgct gatccagaat ccgtacttcg cgcccggcga tggcgtcgtc gaattgctcg
     2221 cgatgatggt ccgccgcggc gtcgccgtgc acttgatggt gccgggcaag cacaccgata
     2281 gtcctttcgt gcggcgcgcg ggctgcagcc tgtatacgcc gatgctcgaa gccggcgtgc
     2341 ggctctacga attcgagccg acgctgttgc atcagaagat cgtcgtcgtg gacgggctct
     2401 ggtcgcacgt ggggtccacg aatttcgacg cgcgctcgct cgcgctcaac gaggaggtcg
     2461 gcgtgggaat cctcgacgcg ggcgtcgccg cagagctcaa agcggcgttt cagcaggact
     2521 tgcgccgttc gcatgaatta aagctcgaag aatggcgccg ccgccccatc tacgatcgcg
     2581 tattcgagcg ctgcgcgtat ctcttgcacg agcagttgta gagacttttc agccggtacc
     2641 gcccggctga cggcgagcgc gtgagtcagc tctgacgacg cgcttttggg tcggttcgca
     2701 caagaggatc tcacggctat acagagtgct tctcatgctt ttataattcc ctataattac
     2761 ctatagaatg gtatatagga aataatagag
//
LOCUS       DASSNN010000388         3505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1816743, whole genome shotgun sequence.
ACCESSION   DASSNN010000388 DASSNN010000000
VERSION     DASSNN010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816743"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VFL84_07140"
     CDS             <1..431
                     /locus_tag="VFL84_07140"
                     /inference="COORDINATES: protein motif:HMM:NF016184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HET7608435.1"
                     /translation="LHTAGQPDAATLATLGDQGFELVVNLAPPNNQGAVPEEGKLVAE
                     DGPTYVNIPVNWQKPTNEDFELFSAVMNGARDRKVLVHCQLNMRASVFTFLYRVIHEH
                     VPPEEAMKALSAVWIPKDQWAAFTADELKRHNIDFELPKP"
     gene            538..1305
                     /locus_tag="VFL84_07145"
     CDS             538..1305
                     /locus_tag="VFL84_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608436.1"
                     /translation="MPKSVSSIALAAALLAVCGCDRAEPQAAAVEAAPQHPDFSGIWF
                     PAGRQQDPQRPELTPGAAAAAAEYEKAFTLDDDPGRYCIWPGMPRAVWGAPFTVEIQQ
                     RPQDVTIYWEGYGMYRKIFMADTAPPVPVLPSAMGYSVAHWEGDVLVVDTTNLKQYPY
                     MTRQATSDAAHVVERIRLEERDVDGKKTKFIVNELVLTDPKVYMTPIKITGTLRHRPD
                     LQPLEYTCTDALWDEYLTERGLSLPDLDALPPVGPQG"
     gene            1311..1691
                     /locus_tag="VFL84_07150"
     CDS             1311..1691
                     /locus_tag="VFL84_07150"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7608437.1"
                     /translation="MKELVNGLLVAALTAVAVPAAAHHSFAIYDMTQNIEFDGVVETL
                     KMRNPHMAMTLTVTNADGTTKTINFVEGAPANMIVRMGLNPADVAPGKAIKAVGAPRK
                     DDANAYFLKAIVLPDGRKYVSVGN"
     gene            complement(1708..1977)
                     /locus_tag="VFL84_07155"
                     /pseudo
     CDS             complement(1708..1977)
                     /locus_tag="VFL84_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421811.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(1965..2486)
                     /locus_tag="VFL84_07160"
     CDS             complement(1965..2486)
                     /locus_tag="VFL84_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608438.1"
                     /translation="MVDAIANFLTSGPIDEFVATHDWAWPLGEILHFFGMALLIGSVG
                     LVDARILGIGKGIPIKSLEKFIPLGVIGFVINVLTGIMFVAGNPVGGPQEYLLNLALQ
                     LKMLCVLIAGLNLLAFYALGIAKKANAVEPGGDAPLSAKVVAVVSLLLWFGVIYFGRF
                     IMYNDTLLYAWGL"
     gene            complement(2505..3002)
                     /locus_tag="VFL84_07165"
     CDS             complement(2505..3002)
                     /locus_tag="VFL84_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608439.1"
                     /translation="MIDAIENSAYAIWVRESPSIWAFTTILSLHAMGLAIIVGINTII
                     ALRLLGYVPSIPVNSLRKLYPWMYVGFTINAFSGLSLLAANLDNDIHNWMFLTKLTLI
                     ACAMINLELTRAKVFDDPLVVNGTGVPASAKKFAIASIVLWTLAIIAGRLSEYPSFVE
                     AWVGF"
     gene            complement(3027..>3505)
                     /locus_tag="VFL84_07170"
     CDS             complement(3027..>3505)
                     /locus_tag="VFL84_07170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7608440.1"
                     /translation="AGGATGGAVLGLLTMRFGLKGLTISVLVLSTVLVAIFGQTPPDL
                     GQLGLVCFFAGFFTNAGIVGLYAIFAHAFPTHVRATGTGFSIGVGRGGSVLAPAVAGF
                     LFQAGYGVPAISILMSLGSLVAAGVLVFLKLTPDVRDEQPADEGAGVPAGLRSRAA"
BASE COUNT          699 a         1138 c         1129 g          539 t
ORIGIN      
        1 ggctgcacac ggccggccaa ccggacgcgg cgactctcgc gacgctcggc gaccagggct
       61 tcgagctcgt cgtgaacctg gcgccgccga acaatcaggg cgccgtgccc gaggaaggca
      121 agctcgtcgc cgaggacggc ccgacgtacg tcaacatccc cgtcaattgg cagaagccga
      181 cgaacgagga cttcgagctc ttcagcgccg tcatgaacgg cgcgcgcgac cgcaaggtgc
      241 tcgtgcattg ccagctcaac atgcgcgcgt ccgtgttcac gttcttgtac cgcgtcattc
      301 acgagcacgt gccgcccgag gaggccatga aggcgctcag tgccgtgtgg attcccaagg
      361 accaatgggc tgcgttcaca gccgacgagc tcaagcgcca caacatcgac ttcgagctgc
      421 cgaagccctg aaacgcaccc agaaaaatta agcagcgctt cccgtgcggc ccggccctaa
      481 ccttctgcgc ccgaggcgcg gacggttatg atgcgtagca ttcgactggg ggttttcatg
      541 ccgaagtccg tatcaagcat cgcgctcgcc gccgctctgc tggccgtgtg cggctgcgac
      601 cgcgccgagc cgcaggccgc cgcggttgag gcggcgccgc agcaccccga cttcagcggt
      661 atctggtttc cggcgggccg gcagcaggac ccgcaacgtc cggagcttac gccgggcgcg
      721 gcggccgcgg cggcggagta cgaaaaggcg ttcacgctcg acgacgaccc cggccgctac
      781 tgcatctggc ccggcatgcc gcgcgccgtc tggggcgcgc cgttcacggt cgagattcag
      841 cagcgcccgc aggacgtcac gatctactgg gaaggctacg gcatgtaccg gaagatcttc
      901 atggccgaca ccgctccgcc cgtgcccgtg ctgccttcgg cgatgggcta ctccgtcgca
      961 cattgggaag gcgacgtgct cgtcgtcgat acgacgaacc tgaagcagta cccgtacatg
     1021 acgcggcaag cgacgtccga cgccgcgcac gtcgtcgagc gcattcggct cgaagagcgc
     1081 gacgtcgacg gcaagaaaac gaagttcatc gtcaacgagc tcgtgctgac cgatccgaag
     1141 gtctatatga cgccgatcaa gatcactgga acgctacggc accgccccga cttgcagccg
     1201 ctcgagtaca cgtgcacgga tgcgttgtgg gacgagtacc tgaccgagcg cggtttgtcg
     1261 ctgcccgacc tcgatgcact tccccccgta ggcccgcaag gttgagtgcc atgaaagaac
     1321 tagtgaacgg tttgctcgtc gcagcgctca ccgcggtggc agtccccgcg gcggcgcatc
     1381 actcgttcgc gatctacgac atgacgcaga acatcgagtt cgacggcgtc gtcgagacgc
     1441 tgaagatgcg caacccgcac atggcgatga cgctgacggt cacgaacgcc gacggcacca
     1501 cgaagacgat caacttcgtc gagggcgcgc ccgcgaacat gatcgtgcgc atgggtctga
     1561 acccggccga cgtcgccccc ggcaaggcga tcaaggcggt cggcgctccg cgcaaagacg
     1621 acgcgaacgc ctatttcttg aaggcgatcg tcctgcccga cggccggaag tacgtgtccg
     1681 tagggaacta gcgcgcaagc tcgcgaacag ggcgcgcttc gccgcgcgct tcaattgctt
     1741 gatctgccat tcgcgccgca gcgcggcgcc gcgatccgcc gccgattcgg aatagacgag
     1801 cttcacgggc aaccgttgcc gagtgtactt cgcgccgcgg ccgtcgttgt gggccgcgat
     1861 acgccgcggc agatctttgg ccacgcccgt gtagagcgtg cgatccgcgc agcgcacgat
     1921 gtagacgtac cagccggcgc gcgcggcgcg cttcggtgcc tgctttacag tccccacgcg
     1981 tacaacagcg tgtcgttgta catgatgaag cggccgaagt agatgacgcc gaaccacagc
     2041 agcagcgaga cgacggcgac gaccttggcg ctcagcggcg cgtcgccgcc gggctccacg
     2101 gcgttggctt tcttcgcgat gccgagcgca tagaacgcca gcaggttcag gccggcgatc
     2161 agcacgcaca gcatcttgag ctgcagcgcg aggttcaaca ggtactcctg cggaccgccg
     2221 accggattgc cggcgacgaa catgatgccc gtgagcacgt tgatgacgaa gccgatgacg
     2281 ccgagcggga tgaacttctc gagcgacttg atcgggatgc ccttgccgat gccgaggatg
     2341 cgggcgtcga ccaagccgac gctgccgatc aagagcgcca tgccgaagaa gtgcaggatc
     2401 tcgccgagcg gccatgccca atcgtgcgtg gccacgaact cgtcgatcgg cccggaggtc
     2461 aagaaattcg caatggcgtc taccacgtgt cgttctccct gaaactagaa gccgacccag
     2521 gcttcgacga agctcggata ctcggacagc cgccccgcga tgattgcgag cgtccagagg
     2581 acgatcgacg cgatggcgaa cttcttcgcg gatgccggaa cgccggtgcc gttgacgacg
     2641 agagggtcgt cgaagacctt ggcgcgcgtg agctcgaggt tgatcatcgc gcacgcgatc
     2701 agcgtcagct tcgtcaagaa catccagttg tggatgtcgt tgtcgagatt ggcggcgagc
     2761 aaggatagcc cggaaaacgc attgatcgtg aagcccacgt acatccacgg atacagcttg
     2821 cgcaggctgt tcacgggaat gctcggtacg tagccgagca gccgcaaggc gatgatcgtg
     2881 ttgatgccta cgatgatcgc gaggcccatc gcatgcagcg acaggatcgt ggtgaaggcc
     2941 caaatggaag gtgattcgcg tacccagatg gcatacgcgc tattttcgat agcgtcgatc
     3001 atagagttgt tatcccagag aaaaaactac gcggcccgac tcctcaaacc cgcgggcacg
     3061 ccggcgccct catcagcggg ctgctcgtcg cgcacgtccg gagtgagctt caggaaaacc
     3121 aagacgccgg ccgcgacgag cgagccgagc gacatcagga tcgagatcgc gggcacgcca
     3181 tagccggcct ggaacaggaa tcccgctacg gccggcgcca gcaccgatcc gccgcgcccg
     3241 acgccgatgg agaacccggt gcccgttgcg cgcacgtgcg tcgggaaggc gtgggcgaag
     3301 atcgcgtaga gcccgacgat accggcgttg gtgaagaagc cggcgaagaa gcagaccagg
     3361 ccgagctggc cgagatcggg cggcgtctga ccgaagatcg cgaccaagac cgtcgaaagg
     3421 acgagcacgg agatcgtcag acccttcaga ccgaaacgca tcgtgagcag accgagcacc
     3481 gctccacccg tggcgccgcc cgcca
//
LOCUS       DASSNN010000389         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_69905, whole genome shotgun sequence.
ACCESSION   DASSNN010000389 DASSNN010000000
VERSION     DASSNN010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69905"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1989
                     /locus_tag="VFL84_07175"
     CDS             <1..1989
                     /locus_tag="VFL84_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7608441.1"
                     /translation="PGSRLRARMISVLVMVAITPLVGVYIFSVEFINRGIDNWLNVDV
                     ERGMSDATNLAKAALDIQTRQRLEEVQRFGNELAGVEGAELARKLNSLRIGSDALEIA
                     AYGNNRVLGISSADIDTDAPLYPNDEVLRQLRQQGEYAVAEPQANGDYRIFASTTIRS
                     STTNGDLGFVQATFPVEQRLSTLLNAVQGASDQVGRLNFFRSALKTSFTLTLSLVLLI
                     SLLASVYGAFYFSRRLVTPIQQLMQGTRAVARGDFATRVPTPARDEIGFLVHSFNDMT
                     QRLARASEDARQSQQQAERERRKLEVILARLSTGVVSLEPDLRIRTANHAASTILGVN
                     LETHVGESIVKLAEKEPLLGQFVGVASTRLSSGEREWREQIMLRAEGGRRVLMCACTE
                     LPGDTDSPNGYAIVFDDITALLQAQRDAAWGEVARRLAHEIKNPLTPIQLSAERLRRK
                     YLKPEGGEAELLDRATHTIIQQVEAMKQMVNAFSEYARSPRMEVSRFDVNALIRELSD
                     LYAHQEKPLSIKLALADDLPPIEADAGRLRQVLHNLLRNALEAMEHQDEARVEVSTRR
                     VTSGDAELVEIKVADNGPGFLRDIVHQAFEPYVTSKPKGTGLGLAIVKKLVEEHGGQI
                     NARNREQGGAEISILIPMSVDANGQSISRRHDHRRERA"
     gene            1986..>2525
                     /locus_tag="VFL84_07180"
     CDS             1986..>2525
                     /locus_tag="VFL84_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7608442.1"
                     /translation="MSARILVVDDEANIRALIDEILSEEGYEVTTAADAKEARSARKR
                     QDFDLVLLDIWMPDTDGISLLREWSEGGSTGTVVMMSGHGTVDTAVEATRLGAVDFVE
                     KPVSLAKLLRTVERALAVRRTKGERRGAAMPSSVPAGKSQPVRALREQLARVAQHSAH
                     TLFTGEPGSGRELFARFLAS"
BASE COUNT          475 a          815 c          839 g          396 t
ORIGIN      
        1 ccgggctcgc gcctacgcgc gcgcatgatc tcggtgctcg tcatggtcgc catcacgccg
       61 ctcgtcggcg tgtacatctt ctccgtcgag ttcatcaacc gcggcatcga caattggctc
      121 aacgtcgacg tcgagcgagg catgagcgac gcgaccaacc tcgcgaaggc ggcgctcgat
      181 atccagacgc ggcagcggct cgaggaagtg cagcgcttcg gcaacgagct cgcaggcgtc
      241 gaaggcgcgg agctcgcgcg caagctgaat tcgctgcgaa taggcagcga cgcgctcgag
      301 atcgcggcct atggcaacaa ccgagttctc ggaatttcct cggccgatat cgacaccgat
      361 gcgccgctgt atccgaacga tgaggtcttg cggcagctgc ggcagcaagg ggagtacgcc
      421 gtcgcggagc cgcaggcaaa cggcgactac cgaattttcg cctcgacgac gatccggtcg
      481 tcgacgacga acggcgacct cgggttcgtg caagcgacgt ttcccgtgga gcagcggctc
      541 agcacgcttt tgaacgcggt gcagggcgct tcggaccaag tggggcggct gaatttcttc
      601 cgctccgccc tgaagacgag cttcacgctg acgttgagcc tcgtgctgct gatctcgctg
      661 ctcgcgtcgg tctacggcgc gttctatttc tcacggcggc tcgtaacgcc gatccagcag
      721 ctcatgcagg gcacgcgcgc cgtcgcgcgc ggcgatttcg cgacgcgagt acctactccc
      781 gcgcgcgacg agatcggctt cctcgtccac tcgttcaacg acatgacgca gcgcctcgcg
      841 cgcgcgagcg aggacgcgcg ccaaagccag cagcaggccg agcgcgagcg ccgcaagctc
      901 gaggtgatcc tcgcgcgcct gtcgacgggc gtcgtgtcgc tcgagcccga cttgaggatc
      961 cggaccgcca atcacgcggc gagcacgatt ctcggcgtca atctcgagac tcacgtcggc
     1021 gagtccatcg tgaagctcgc cgagaaggag ccgttgctcg gacagttcgt cggcgtcgcg
     1081 agcacgcgcc tttcgagcgg cgagcgcgag tggcgcgagc aaatcatgct gcgcgccgaa
     1141 ggcggccgcc gcgtgctgat gtgcgcctgc accgagctgc caggcgatac cgacagcccg
     1201 aacggctacg cgatcgtgtt cgacgacatc acggcgctct tacaggcgca gcgcgacgcc
     1261 gcatggggcg aagtcgcgcg ccggctcgcg cacgagatca agaatccgct gacgccgatt
     1321 cagctctcgg ccgagcggct gcggcgcaag tatctgaaac cggaaggcgg cgaggccgag
     1381 ctgctcgaca gagcaacgca cacgatcatc cagcaagtcg aagccatgaa gcagatggtg
     1441 aacgcgttca gcgagtacgc gcgcagtccg cgcatggagg tctctcgctt cgacgtcaat
     1501 gcgctgatca gagagctatc cgatttgtac gcgcatcagg agaagccgct ttcgatcaag
     1561 ctcgcgctgg ccgacgatct gccgccgatc gaagccgacg ccggccgttt gcggcaagtg
     1621 ctgcacaact tgttgcgcaa tgcgctcgag gcgatggagc atcaagacga agcgcgcgta
     1681 gaggtctcta cgcgccgagt cacgagcggc gacgcggagc tcgtcgagat caaggtcgcg
     1741 gataacgggc ccggcttttt gcgcgacatc gtgcatcagg cattcgagcc gtacgtcacg
     1801 agcaaaccga agggaacggg tctcggcttg gccatcgtca agaagctcgt cgaggagcac
     1861 ggcggtcaga tcaacgcgcg caaccgcgag cagggcggag ccgagatcag tatactcatt
     1921 ccaatgtcag tcgacgcgaa cggtcagtcg atcagtcggc gtcacgatca caggagagaa
     1981 cgcgcatgag tgcgcgaatt ctggttgtcg acgacgaagc caacattcga gccctcatcg
     2041 acgagatcct gagcgaagag ggctacgagg tcacgaccgc tgccgacgcg aaggaagcgc
     2101 gcagcgcccg caagaggcaa gacttcgatc tggttctgct cgacatctgg atgccggaca
     2161 cggacggaat ctcgctgctg cgcgagtggt ccgagggcgg atcgaccggc accgtcgtca
     2221 tgatgtcggg tcacggcacc gtggacacgg ccgtcgaggc gacgcggctc ggcgccgtcg
     2281 acttcgtcga gaagcccgtc tcgctcgcaa agttgctgcg cacggtcgaa cgcgcgctcg
     2341 ccgtccgccg cacgaagggc gaacgccgcg gcgcggccat gcccagctcg gttcccgcgg
     2401 gcaagagcca gccggtgcgt gcgctgcgag agcagctcgc gcgcgtcgcg cagcacagtg
     2461 cgcacacgtt gttcacgggc gagcccggca gcggccgcga gctgttcgca cgttttctcg
     2521 cgagc
//
LOCUS       DASSNN010000390         6841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_446251, whole genome shotgun sequence.
ACCESSION   DASSNN010000390 DASSNN010000000
VERSION     DASSNN010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446251"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VFL84_07185"
     CDS             <1..900
                     /locus_tag="VFL84_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET7608443.1"
                     /translation="LMAAGRLLRRLQRPGIVYCATTVEVDRVYGALVRAGIPADHYHG
                     KMRKADREAAQERYMRPNKRKVMVATSAFGMGIDKPNIRYVLHYQAPSSLEQYVQEAG
                     RGGRDGRPCYCVLLLDPADLDVQRHLLAQGRPRPDQLRSVARALKAWGGERRAVSAAH
                     LADSAGVPVNAARSLCARLEELALVEQTGDKEWSISAPLDAFEAGAEDLARRLDIERL
                     ADERRLDVVREYAATSDCRSVFLRRYFGEENPPRCGVCDRCRAAARDAPRPPSPRAAS
                     GEAGPAAAVEPRRGRRRRRRRRS"
     gene            999..2903
                     /locus_tag="VFL84_07190"
     CDS             999..2903
                     /locus_tag="VFL84_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7608444.1"
                     /translation="MKLRHVMLVPALCLATIAAAQPPPVSAFAALPAIKSPAISPDGK
                     RYAFIAHAGDGVLVYAAQLDNNQADAVINVLDADARALTWANDDTLILLASDTETTVF
                     AARKTEIFTPWGIDLTGDLRIRQLGGARAVGGGLFVRGRRLIGFERATGRVLVPIGGA
                     LYAVNAKNGDVDERDEASGFNRDWVVDENGGPVYRVQYREKTDYFQILRRGDRGLWQT
                     LGATNMELPELDIHGLDDKGELIVGVRPLDSGLYGLYTVTADGKLGQPVFTHATLDVA
                     DVRTDPYTNRVVGAAVEGESPVWFDADLAMEQAALDEAFPNESPWIEDWSQDRSRFIV
                     RTERDDRAPAFYFFDAKTRMAKGLASTYPALDEIRLAPRSAYTYKARDGVEIPGYLAR
                     PVGVDGPAPLVVLPHGGPADRDVDGFDWLAHFLASRGYAVLQPNFRGSGGYGRAWEEA
                     GHGGWGIGVMQNDLSDGVAAVVAAGIADPERVCIVGASYGAYAALAGAAFTPELYRCA
                     VSINGVSDLRDIFTLYRGRRDGRSAAVTYFEQSLGVGDGSPRALDAASPAKHADRVQA
                     AVLLIHGRDDSVVLSGQSRNMERALREAGKTVQYVQLDGEDHWLSQAKTRLETLQAVD
                     KFLGQQLGSR"
     gene            complement(2955..4313)
                     /locus_tag="VFL84_07195"
     CDS             complement(2955..4313)
                     /locus_tag="VFL84_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET7608445.1"
                     /translation="MLVLQLVAVSGEAQQSADRIYVRRIEFRGVTRTNDEVLRRELRQ
                     LEGTFLNPAALEESRIHLERLPSVARAMVEVVPVPGSDNVIDVVVTITEEPTRRYGVG
                     GGYASSLGTSAHGYFVNDNLFGSGQQMSIAMDVSELGSIAEISHTNPYAFDAGVGRTI
                     SLVSRGIDRLTADTSSIDADLVEGRLEYGYALSPAQSVRLGLALRSTSLDAGPDTSAQ
                     LTSWIAANGEPIVTDGAPSTELDELDLTLRWRRDTRNHESFPDAGLEQSIDLRAALPA
                     SEVEYFTLRYDATRYWTVGRQWTLSAHGAVAFGDSLGATTALPPYLNWFAGGPSTVRG
                     FRGLGPEDSLGNPYGGNLLAAGQLDLKTAWPRRWSSWLRSGFFLDAGNVFSTDDTVFF
                     GAGGTPVDHGFSASELRASAGIAADLLLPFGTVRLSYGVPLNAEDGDREERFQVSFGV
                     DF"
     gene            complement(4463..5704)
                     /locus_tag="VFL84_07200"
     CDS             complement(4463..5704)
                     /locus_tag="VFL84_07200"
                     /inference="COORDINATES: protein motif:HMM:NF025755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HET7608446.1"
                     /translation="METTGCDRTPRHGSTESALDGERDRRPTWRERLTRRLGRHLRGL
                     KSAWHDSAVAARQHRRKRVALLAEAASLALRGRMAVDTYFHYRLFDRKLTRAAKRRYL
                     AEAPHANERLWHALTPREYACLYGNKLIFHRFFESIGLPLAALFGVFDPAVGSTEEGR
                     PLRSAADLNELLRQRANEGFVLKPVEGVRGHLTLVFSGPAADSRDTYVTLAGERYDAA
                     ALVAATRRTAALKSQSPDARLSSFLIQERIRPHPELVDFIGPTLCTARVLTIVARDGS
                     PRIVGSVLKVQPSAVGVDHLLYGAVACWIDPDTGVLGPGRTRTHCDYVTTVPGTERPL
                     VGYRVPLWAEVKRLALDAAAAFPWARAIGWDIAVAERGAVLIEGNARWSPSLVQLPAP
                     HGLMDGELEALYRSRRRGDAA"
     gene            complement(5787..>6841)
                     /locus_tag="VFL84_07205"
     CDS             complement(5787..>6841)
                     /locus_tag="VFL84_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HET7608447.1"
                     /translation="WPRPAGSAPAARLFSDGRFFTASRKARLVPTPPRAPARQPDADY
                     PLVLNTGRVRDQWHTMTRTGKSPRLSGQHAEPFVQLHPLDAARFGLALGDLAEVASAG
                     ARIVVRLEITDAVAPGQAFVPMHWGDVFASNARACKLIRSAVDPVSGQPELKAAPVRV
                     RRYEPKWYGFALAREPLAPPPVSYRAVSRGEGYWRYELAGEQLPKSWPEWADAVLGAR
                     GARIELSDVGGGRYRGANVDGERLQACVFVATAKSLPSRHWLATLFGAPKISDADRLA
                     ILAGRAPKGALEAGPVVCSCFAVGRSTLLRAIRGDKLASLEHIGRALRAGTNCGSCIP
                     ELKGLLAESAASAAAP"
BASE COUNT         1068 a         2419 c         2377 g          977 t
ORIGIN      
        1 ctcatggcgg ccggtcggct gctgcgccgc ctgcagcgcc ccgggatcgt gtattgcgcg
       61 acgaccgtcg aggtcgaccg cgtgtacgga gcgctcgtgc gcgcgggcat tcctgccgat
      121 cactaccacg gcaagatgcg caaagccgac cgcgaggccg cgcaggagcg ctacatgcgg
      181 ccgaacaagc gcaaggtgat ggtcgcgacg agcgctttcg gcatgggcat cgacaagccg
      241 aacattcgct acgtgctcca ctaccaggcg ccgtcgtcgc tcgagcaata cgtccaggag
      301 gcgggccgcg gcggtcgcga cggccgtcct tgttactgcg tgctgctgct cgatcccgcc
      361 gatctcgacg tgcagcggca cctgctcgcg cagggccgcc cgcggcccga tcagctgcgc
      421 agcgtcgcgc gggcgctgaa agcgtggggc ggcgagcgcc gcgcagtgag cgccgcgcac
      481 ctcgccgatt ccgccggggt gccggtcaac gcggcgcgct cgctgtgcgc gcggctcgag
      541 gagctcgcgc tcgtcgagca aacgggcgac aaggagtggt cgatctccgc cccgctcgac
      601 gcgttcgagg ccggggccga agacctcgca cgccgcctcg acatcgaacg cctcgccgac
      661 gagcgccggc tcgacgtcgt gcgcgagtac gcggcgacga gcgactgccg cagcgtcttt
      721 ttgcgccgct acttcggcga agagaatccg ccgcgctgcg gcgtctgcga tcgttgccgg
      781 gcggccgccc gcgacgcgcc gcggccgccc tcgccgcgcg cggcctccgg cgaagcgggg
      841 cccgcggcgg cggtcgaacc gcggagaggt cggcgtcgac gccggcgccg ccgatcgtag
      901 gaacagtagg tactccggcc aacaggccga cagctcggcg cccgatatag tcggagcgcg
      961 catcgatccc gatgcggggt tcaaggctcg gagccgagat gaagttgcgc catgtcatgc
     1021 tcgtgcccgc gctgtgcctg gcgacgatcg ccgccgcgca gccgccgccc gtgtccgcgt
     1081 tcgcggcgct gccggccatc aagtcgccgg cgatctcgcc ggacggcaag cgctacgcgt
     1141 tcatcgccca cgccggcgac ggagtcttgg tgtacgccgc gcagctcgac aacaaccagg
     1201 ccgacgccgt catcaacgtc ctcgacgccg acgctcgcgc gctgacgtgg gccaacgacg
     1261 acacgctgat tctgctcgcg agcgataccg agacgacggt cttcgccgcg cgcaagaccg
     1321 aaatattcac accgtggggc atcgacctga ccggcgacct gcggattcgg caattgggcg
     1381 gcgcgcgcgc cgtcggcggc ggtctgttcg tgcgcggccg gcgtctgatc ggcttcgagc
     1441 gcgcgaccgg gcgcgtgctg gtgccgatcg gcggagccct ctatgccgtc aacgccaaga
     1501 acggcgacgt cgacgagcgc gacgaagcgt cgggcttcaa ccgcgactgg gtcgtcgacg
     1561 agaacggcgg accggtgtac cgcgttcagt acagagagaa gaccgactat tttcagatcc
     1621 tgcgccgcgg cgaccgcggt ctgtggcaga cgctcggcgc gacgaacatg gagctgcccg
     1681 agctcgacat ccatgggctc gacgacaagg gcgagctcat tgtcggcgtg cggccgctcg
     1741 atagcggcct gtatgggctc tacacggtga ccgccgacgg caagctcggg cagccggtgt
     1801 tcacgcacgc gacgctcgac gtcgccgacg tgcgcaccga tccgtacacg aaccgcgtcg
     1861 tcggagccgc cgtcgagggc gagagcccgg tctggttcga cgcggatctc gccatggagc
     1921 aagcggcgct cgacgaagcg ttcccgaacg agtcgccttg gatagaagat tggtcgcagg
     1981 atcgctcgcg gttcatcgtc cgcaccgagc gcgacgatcg cgcaccggcg ttctatttct
     2041 tcgacgcgaa gacgcggatg gcgaaggggc tcgcgagcac ctatcccgcg ctcgacgaga
     2101 ttcgcctcgc gccgcgctcg gcgtacacgt acaaggcgcg cgacggcgtc gagattccgg
     2161 gctatctcgc gcggccggtc ggcgtcgacg gccccgcccc gctcgtcgtg ctgccgcacg
     2221 gcggccccgc cgaccgcgac gtcgacggct tcgactggct cgcgcatttc ctcgcgtcgc
     2281 gcggctacgc cgtgctgcag ccgaattttc gcggctcggg cggctatggc cgcgcctggg
     2341 aggaagcggg acacggcggt tggggcatcg gcgtcatgca gaacgacttg agcgacggcg
     2401 tcgcggccgt cgtcgcggcc ggcatcgcgg acccggagcg cgtttgcatc gtcggcgcga
     2461 gctacggcgc gtacgcggcg ctcgcgggcg ccgcgttcac gccggagctc tatcgctgcg
     2521 cggtctcgat caacggcgtc tccgacttgc gcgacatctt cacgctctat cgcggccgca
     2581 gggacggccg cagcgccgcg gtcacgtact tcgagcagtc gctcggcgtc ggcgacggct
     2641 cgccgcgcgc gctcgacgcg gcctcgcccg cgaagcatgc cgaccgagtg caggcggccg
     2701 tgctgctgat ccacggccgc gacgattccg tcgtgctgag cggccagagc cggaacatgg
     2761 agcgcgcgct gcgcgaagcg ggcaagacgg tgcaatacgt ccagctcgac ggcgaggatc
     2821 actggctctc tcaggcgaag acgcgcctcg agacgttgca agccgtcgac aaattcctcg
     2881 gtcagcagct cggcagccgc tagccgcccc tgctgcattc ctgccgcggt atttcgctgc
     2941 cgcttcaagc tcgttcagaa atcgacgccg aagctgacct ggaatcgctc ttcgcggtcg
     3001 ccgtcttcgg cattcagcgg caccccgtag ctcaggcgca cggtgccgaa cggtagcagc
     3061 aagtcggctg caatgccggc ggaggctcgc agctccgacg cgctgaagcc gtgatcgacg
     3121 ggagtgccgc cggcgccgaa gaacacggtg tcgtccgtcg aaaacacgtt gccggcgtcg
     3181 aggaagaaac cggagcgcag ccacgaagac cagcggcgcg gccacgccgt tttcagatcg
     3241 agctgcccgg ccgcgagcaa attgccgccg tacgggttgc cgagactgtc ctcgggaccg
     3301 agccccctga acccacgaac ggtgctcggt ccgcccgcga accaattcaa gtagggcggc
     3361 aatgccgtcg tcgcgccgag gctgtcgccg aacgcgacgg cgccgtgcgc gctcagcgtc
     3421 cattgccggc cgacggtcca gtagcgcgtc gcgtcgtagc gcagcgtgaa gtattcgact
     3481 tcgctcgccg gtaacgccgc gcgcagatcg atcgactgct cgagccctgc gtcgggaaag
     3541 ctctcgtggt tacgcgtgtc gcgccgccag cgaagcgtca gatcgagctc gtcgagctcg
     3601 gtcgacggcg cgccgtcagt gacgatcggc tcgccgttcg cggcgatcca actcgtcagc
     3661 tgcgcgctcg tgtcggggcc cgcgtcgagc gacgtgctgc gcagcgcgag cccgaggcgc
     3721 acgctctgtg ccggcgagag cgcatagcca tactccaacc ggccctcgac caggtcggcg
     3781 tcgatgctcg aggtgtccgc cgtcagccgg tcgatgccgc gcgagaccaa gctgatcgtt
     3841 cgaccgaccc ctgcgtcgaa cgcgtaagga ttcgtgtgcg agatttccgc gatcgatccg
     3901 agctcgctga cgtccatcgc gatggacatc tgctgccccg acccgaacaa attgtcgttg
     3961 acgaagtagc cgtgcgcgct cgtcccgagc gagctcgcgt acccgccgcc gacgccgtag
     4021 cgccgtgtcg gctcttccgt gatcgtcacg acgacgtcga tcacgttgtc cgaccccgga
     4081 accgggacga cctcgaccat ggcgcgcgcg acggacggca accgttcgag atgaatgcgc
     4141 gattcctcga gcgccgccgg attcaggaag gtgccctcga gctgccgcag ctcgcgccgg
     4201 agcacctcgt cgttcgtccg cgtgacgccg cgaaactcga tgcgcctgac gtagattcgg
     4261 tcggcggact gctgcgcctc gcccgatacc gcgacgagct gcaaaaccag cacgagtagg
     4321 accggccgaa cgaagcgcaa gcgacggaca tgcctccgtg tcacatcgac cgttcgatga
     4381 cggcgtaaag gtacggttcc cgatgacatc cgtccgcgga ggggttctcg aaactacttt
     4441 aagctcgttc agctacgcgt ggtcaggccg catcgcctct gcgacgcgag cggtatagcg
     4501 cttctagctc gccgtccatg agcccgtgcg gcgccggcag ctggaccagc gaaggcgacc
     4561 agcgcgcgtt gccctcgatc agcaccgccc cgcgctccgc gacggcgatg tcccagccga
     4621 tcgcgcgcgc ccaggggaag gccgccgcgg cgtcgagagc caggcgcttg acctccgccc
     4681 atagcgggac cctatagccg acgagcggcc gctcggtgcc gggcaccgtc gtgacgtagt
     4741 cgcagtgcgt ccgggtccgg cccggcccga gaacgccggt atcggggtcg atccagcacg
     4801 cgaccgcgcc gtagagcaag tggtcgacgc cgaccgcgga cggctgcacc ttgagcacgc
     4861 tgccgacgat tcgcgggctg ccgtcgcgcg cgacgatcgt gagcacacgc gccgtgcaca
     4921 acgtcggccc gatgaagtcg acgagctccg gatgcggacg aatgcgctcc tggatcagga
     4981 acgacgacaa ccgcgcgtcg ggtgattggc tcttgagcgc cgccgtccgc cgagtcgccg
     5041 cgacgagcgc cgcggcgtca tagcgctcgc cggcgagcgt gacgtaggta tcgcgcgaat
     5101 ccgcggcggg accgctgaac acgagagtca gatggccgcg cacgccttcg accggcttca
     5161 agacgaagcc ctcgttcgcg cgctgccgca atagctcgtt caaatcggcg gccgaccgca
     5221 acggccggcc ttcctcggtg ctgccgaccg ccggatcgaa cacgccgaac agcgcggcga
     5281 gcggcaagcc gatcgactcg aagaagcggt ggaagatcag cttgttgcca tagagacacg
     5341 cgtactcgcg aggggtcagc gcgtgccaaa gccgctcgtt cgcgtgcggg gcctcggcaa
     5401 gataacggcg tttcgcggcg cgcgtcagct tccggtcgaa cagccggtaa tggaaatagg
     5461 tgtcgacggc catgcgtccg cgcagcgcga gcgacgccgc ttccgcgagc agcgcaacgc
     5521 gcttgcgccg atgctggcgc gccgcgacgg cggaatcgtg ccacgccgat ttgagcccgc
     5581 gcaggtggcg cccgaggcgc cgcgtcagcc tctccctcca tgtcggtcga cggtcgcgct
     5641 cgccgtcgag cgcactctcg gtcgagccat gacggggcgt tcgatcgcag ccggtcgtct
     5701 ccatggctcc ctccgtccga ggcgacgatg agcgcatcag ggtgcaattc ccatgccgcc
     5761 aatgacgcgc acgggctggc gccgtgctag ggcgcggcgg cgctcgcggc agactcggcg
     5821 agcaagcctt tgagctcggg aatgcacgag ccgcagttcg tgccggcacg cagcgctcgg
     5881 ccgatgtgct cgagcgaggc gagcttgtcg ccgcggatcg cgcgcagcag cgtcgagcgg
     5941 ccgaccgcga agcacgagca gacgacgggg ccggcctcga gcgccccctt cggcgcgcgc
     6001 ccggcgagga ttgcgagtcg atcggcgtcg gagatcttcg gcgcgccgaa cagcgtcgcg
     6061 agccaatggc gcgacggcag cgacttcgct gtcgcgacga acacgcacgc ttgcagccgc
     6121 tcgccgtcga cgttcgcgcc gcgatagcgc ccgcccccga cgtcggagag ctcgatgcgc
     6181 gcgcctcgcg cgcccaagac cgcgtcggcc cactcgggcc acgacttcgg cagctgctcg
     6241 cctgcaagct cgtagcgcca gtagccttcg ccgcgcgaga cggcgcgata cgaaaccggc
     6301 ggcggcgcga gcggctcacg cgccaaagcg aagccgtacc acttcggctc gtaacgccgc
     6361 acgcggaccg gcgccgcttt gagctcgggc tgcccggaca cggggtccac ggcggaacgg
     6421 atcaacttgc acgcgcgcgc gttgctcgcg aacacgtccc cccaatgcat cggcacgaac
     6481 gcctgccccg gcgccacggc gtccgtgatc tcgaggcgca cgacgatgcg cgcgccggcg
     6541 ctcgcgacct cggccaaatc gccgagcgcg aggccgaagc gcgccgcgtc gagcgggtgc
     6601 agctgcacga acggctcggc gtgctggccc gagagccgcg gcgacttgcc cgtgcgcgtc
     6661 atcgtgtgcc actgatcgcg gacgcggccc gtgttcagca cgagcggata gtcggcatcg
     6721 ggttgccgcg ccggcgcgcg cggcggcgtc ggcacgagcc gcgctttgcg gctcgccgtg
     6781 aagaagcggc cgtccgagaa cagcctcgcc gccggagcgg agccggcggg ccgcggccat
     6841 t
//
LOCUS       DASSNN010000391         3881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_672187, whole genome shotgun sequence.
ACCESSION   DASSNN010000391 DASSNN010000000
VERSION     DASSNN010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672187"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /gene="purN"
                     /locus_tag="VFL84_07210"
     CDS             complement(<1..463)
                     /gene="purN"
                     /locus_tag="VFL84_07210"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET7608448.1"
                     /translation="MTREAARVAVLISGEGSNLQALIDAEHAGQLGARIVTVVSNRGA
                     ARGLERARAAGIEALHLAPKRGQERADYDAALARLLAPRAPDLIVLAGFMRILGPALV
                     ERYAGRMLNLHPSLLPKYPGLDTHRRVLEAGDERHGATVHFVTDELDGGPAI"
     gene            537..1613
                     /locus_tag="VFL84_07215"
     CDS             537..1613
                     /locus_tag="VFL84_07215"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HET7608449.1"
                     /translation="MGSSSSLHRSLAAALLVVAAWAPPSGAATLANLYTITVPADPAA
                     ADQREAATQAAMAGVLIRITGSRNAPVDPALQPLIAKPDAYLSSYGVDRQGRALVGFS
                     PGQIERVLTELGKPVWGPERPLTLLWVAIDDGAGGRALLGAGESSDCGMPMTPRMAEQ
                     SGNVREEIVAAAEERGVPIAWPLLDLQDLDAVTCTDVWGGFEDEIVAASARYRADAVL
                     IGKARPGTFGTEVEWLFVHGLERQPLPLAGVRDGIDSAADRYAADLATVGGASLTLLT
                     VSNVLTPADYGRVVSYLQRQSVLETVDVESLDNGTLRLRVAARGDARVLERVLALGGV
                     LRPATAASSFGGLNFEVAPGGSSP"
     gene            1610..2167
                     /locus_tag="VFL84_07220"
     CDS             1610..2167
                     /locus_tag="VFL84_07220"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7608450.1"
                     /translation="MSLRFIPNAICVARMLLVAPLVLWIVEGRFAAALIVLVVAGLSD
                     GLDGFLAKRFDWQTRIGGLLDPLADKLLLVAAFASLTYVGLVPLELTAVVVARDVVIV
                     LGAVCYQWLIAPVQGEPAAISKLNTACQLAMLFFTLTAAVFAWPPPVSLLVLGATVVF
                     TSIVSGLTYVLRFTSRAWRVAHGAP"
     gene            2181..2876
                     /gene="hda"
                     /locus_tag="VFL84_07225"
     CDS             2181..2876
                     /gene="hda"
                     /locus_tag="VFL84_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HET7608451.1"
                     /translation="MGQLPLALALENHASFETFVAGSNAAAVEHVARLARGTADTVWL
                     WGAEGSGKTHLLQAACREASASGRRAMYVALPAVSPEILSGLERVDVLALDEPQAVAG
                     DLAWEQALFVILNAFSSRSGGLLLAAPSPASQCGFRLADLASRGAGAVTYRLATLDDA
                     ERAAALRLHAAARGLTLDAAAADFLLTRVARDMGALTGWLKRLDRASLTAQRRLTIPF
                     IRELMARSEPSAD"
     gene            complement(2884..3483)
                     /gene="wrbA"
                     /locus_tag="VFL84_07230"
     CDS             complement(2884..3483)
                     /gene="wrbA"
                     /locus_tag="VFL84_07230"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HET7608452.1"
                     /translation="MTQTTVLVLYYSRGGRTAALARQAARGVESAGAVARLRTVPPVT
                     AATPGDLPAVPADGPPYAELADLADADGLLLGSPTRFGNMAAPLKHFLDTTSELWLSG
                     RLKDKPAGVFTSSQSAHGGQESTLLSMQLPLLHHGMLIVGVPYTEPALFETRTGGSPY
                     GATHVASSWNTSLSAEESAVARVLGARVARIAQALAQLR"
     gene            complement(3480..3824)
                     /gene="arsC"
                     /locus_tag="VFL84_07235"
     CDS             complement(3480..3824)
                     /gene="arsC"
                     /locus_tag="VFL84_07235"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263306.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HET7608453.1"
                     /translation="MKAKIYHNPRCSKSRATLAILQQRGVELEIIDYIATPPSKDVLR
                     ALLKKLGGPALAMVRTEDQEFRPHAQRALSDEEVIDLLAREPRLLQRPIVEIGKAARI
                     GRPPERVLELIE"
BASE COUNT          500 a         1327 c         1419 g          635 t
ORIGIN      
        1 tgatcgccgg gccgccatcg agctcgtcgg tcacgaagtg gacagtggcg ccgtgccgct
       61 cgtcgccggc ctcgagcacg cgccgatgcg tatcgagccc cggatacttc ggaagcagcg
      121 atgggtgcaa gttcagcatg cggccggcat agcgctcgac gagcgcgggg ccgaggatcc
      181 tcatgaagcc cgcgagcacg atcagatccg gcgcgcgcgg cgcgagcaga cgggcgagag
      241 cggcgtcgta gtcggcgcgc tcttggcctc gcttgggcgc gagatgcagc gcttcgatgc
      301 cggcggcccg cgctcgctcg aggccgcgcg cggcgccgcg gttgctgacg accgtgacga
      361 tccgggcgcc gagctgcccg gcgtgctcgg cgtcgatcaa cgcttgcagg ttgctgcctt
      421 cgccggagat cagcacggcg acgcgcgcgg cttcccgtgt catcgactgg ctctcggcga
      481 ggcgctatgg gtgggcggct ataatacggg acagttcgtc gtcatgggca caccgcatgg
      541 gaagttcatc gtcgctgcac cggtcgcttg ccgccgcgct gctcgtcgtg gctgcttggg
      601 cgccgccgag cggcgccgcg actctcgcga acttgtacac gatcacggtc cccgcggacc
      661 cggcagccgc cgaccaacgc gaggcggcga cgcaggccgc gatggcgggc gtgctgattc
      721 gcatcacggg aagccgcaac gcgccggtcg atccggcgct acagccattg atcgccaaac
      781 ccgacgcgta cttgagctcg tacggcgtcg accgtcaggg ccgcgcgctc gtcggcttca
      841 gcccgggcca gatcgagcgc gtgctcaccg agctcggcaa gccggtgtgg ggaccggagc
      901 ggccgttgac gctgttgtgg gtcgcgatcg acgacggcgc gggcggccgc gcgctgctcg
      961 gcgccggcga gtcgtcggac tgcggcatgc cgatgacgcc gcgcatggcg gagcagagcg
     1021 gcaacgtgcg cgaggagatc gtcgccgcgg cggaggagcg cggggtgccg atcgcgtggc
     1081 cgttgctcga ccttcaagac ttggacgccg tcacgtgcac ggacgtctgg ggcgggttcg
     1141 aggacgagat cgtggccgca tcggcgcgct atcgcgcgga cgccgtgctg atcggcaaag
     1201 cgcgcccggg aacgttcggc accgaggtcg aatggctgtt cgtgcatggc ctcgagcgcc
     1261 agccgctgcc gctcgcgggc gtacgagacg gcatcgactc cgccgcggat cgctacgccg
     1321 ccgatcttgc gacggtcgga ggggcaagcc tcacgttgct gaccgtcagc aacgtgctca
     1381 cgcccgccga ctacggtcgc gtcgtcagct acttgcagcg gcagagcgtt ctcgagaccg
     1441 tcgacgtcga gtcgctcgac aacggtacgc tgcgcttgcg cgtcgcggcg cgcggcgacg
     1501 cgcgcgtgct cgagcgcgtg ctcgcgctcg gaggcgtgtt gcggccggct accgccgcga
     1561 gctcgttcgg cgggctcaac ttcgaggtgg ctcccggcgg ctcttcgcca tgagccttcg
     1621 gttcatcccg aacgcgatct gcgtcgcgcg gatgctgctc gtcgcgccgc tcgtgttgtg
     1681 gatcgtcgaa ggccgtttcg ccgcggcgtt gatcgtgctc gtcgtggcgg ggctgtccga
     1741 cgggctcgac gggttcctcg cgaagcgctt cgactggcag acgcgcatcg gaggcttgct
     1801 cgatccgctg gccgacaagc tgctcctggt cgcggcgttc gcgtcgctga cctacgtcgg
     1861 cctcgtgccg ctcgagctca cggccgtcgt cgtcgcgcgc gacgtcgtga tcgtgctcgg
     1921 cgcggtctgc taccaatggc tcatcgcgcc cgttcaaggc gagcccgccg cgatcagcaa
     1981 gctgaacacg gcgtgccagc tcgcgatgtt gttcttcacg ctgacggccg ccgtgtttgc
     2041 gtggccgccg cccgtgtcgt tgctggtgct cggcgctacc gtcgtattca cgagcatcgt
     2101 cagcgggctg acttacgtgc tgcgcttcac ctcgcgcgcg tggcgcgtgg cgcacggcgc
     2161 gccttgagcg gagagcgccg ttggggcagc tgccgctcgc attggcgctc gagaatcacg
     2221 cgagcttcga gacgttcgtc gccggcagca atgccgcggc cgtggagcac gtagcccgcc
     2281 tcgcgcgcgg caccgccgac accgtttggc tgtggggcgc cgagggctcc ggcaagactc
     2341 acttgctgca ggccgcgtgc cgcgaggcat ccgcttcggg aaggcgcgcg atgtacgtcg
     2401 cgctgcccgc cgtgtcgccc gagatcttga gcggcctcga gcgcgtcgac gtgctcgccc
     2461 tcgacgagcc gcaggcggtt gccggcgatc tcgcgtggga gcaggcgctg ttcgtgatcc
     2521 tgaacgcgtt ctcgagccgc agcggcggtc tcctgctcgc ggcgccttcg cccgcgtcgc
     2581 aatgcggctt tcgcctcgcg gacctcgcct cgcgcggcgc gggtgccgtg acgtatcggc
     2641 tggcgacgct cgacgacgcc gagcgcgccg cggcgctgcg gctccatgcc gcggcgcgcg
     2701 gtctcacgct cgacgccgcg gcggcggatt ttctgctgac gcgcgtcgcg cgcgacatgg
     2761 gcgcgctcac gggctggctc aagcggctcg atcgcgcttc gctgacggcg cagcggcgat
     2821 tgacgatccc gttcatccgc gagctcatgg ctcgttccga gccgagcgcc gactgagcgc
     2881 gcgctagcgc aattgcgcca gtgcttgcgc gatgcgagcg acgcgcgctc cgagcacacg
     2941 cgcgacggcc gactcctcgg ccgacagaga cgtgttccac gacgacgcga cgtgcgttgc
     3001 gccgtacggc gagccgccgg tccgcgtctc gaacagcgcg ggctcggtat acggcacgcc
     3061 gacgatcagc atgccgtggt gcaggagcgg caattgcatg ctgagcaaag tgctctcctg
     3121 cccgccgtgc gcggactggc tcgacgtgaa cacgccggcc ggcttgtcct tgagccgccc
     3181 ggacagccag agctcgctcg tcgtgtcgag gaagtgcttg agcggcgcgg ccatgttgcc
     3241 gaagcgcgtc gggctgccga gcagcaggcc gtccgcgtcc gcgaggtcgg cgagctcggc
     3301 gtagggaggg ccgtcggccg gcacggcggg aagatcgccc ggcgtcgccg ccgtcaccgg
     3361 cgggacggta cgcaagcgcg cgacggctcc ggcgctctcg acgccgcgcg ccgcttgccg
     3421 cgcgagcgcg gccgtgcgcc cgccgcgcga gtaatacaag acgagcactg tcgtttgcgt
     3481 cactcgatga gctcgagcac gcgctcgggc gggcggccga tgcgcgccgc cttgccgatc
     3541 tcgacgatcg gccgctggag caagcgcggc tcgcgcgcga gcaggtcgat gacttcctcg
     3601 tcgctgagcg cccgctgtgc gtgcggccga aactcctggt cctcggtgcg aaccattgcg
     3661 agcgccggtc cgccgagctt cttgagcagc gcgcgtagca cgtctttcga cggcggcgtc
     3721 gcgatgtagt cgatgatctc gagctcgacg ccgcgctgtt gcaggatggc gagcgtcgcg
     3781 cgcgatttgc tgcagcgcgg attgtggtag attttggcct tcatagcagg ccagtgtaag
     3841 gtttgcgcgt tacgacgaga accgtggcag cgcttttgac g
//
LOCUS       DASSNN010000392         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1817144, whole genome shotgun sequence.
ACCESSION   DASSNN010000392 DASSNN010000000
VERSION     DASSNN010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817144"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFL84_07240"
     CDS             complement(<1..320)
                     /locus_tag="VFL84_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608454.1"
                     /translation="MRAKWSALVLAALALAACAPESPPPESSAAPGELVPADRAAKIA
                     LGLEHATAADLYAALKARANGGAPLSLDDMPDWTGLWQRVGRPFFDPDQPADVLTTAK
                     LRPEP"
     gene            complement(340..1248)
                     /locus_tag="VFL84_07245"
     CDS             complement(340..1248)
                     /locus_tag="VFL84_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608455.1"
                     /translation="MSTVSRRSFPVLVPKICIVALGTLVASASFAAIELPADKAALIA
                     LGEKHRTAWDLYKDFEKQANGGQRLNPARLPDWTGVYSRSGVLFNWDQDQGRNRMPTA
                     KLTPEFERRLVTKLDNIEKDIEFDNLSNCAPPGHPRWMTEPFLRDFVVTPDQTYLINE
                     MMNDIRRIYTDGREHTPEADRYPLWNGDSIGFWDGGKLVIHTNSLRSGQYQRTQPEYT
                     EQVETVEIWEKVDDKTLLAHVWVYDPPSLAQPWYSKHTYLKLTNDDKSLRIRYWDCGE
                     NQNNTVIQTENGSSQFRDFTFTDKDN"
     gene            1300..>2139
                     /locus_tag="VFL84_07250"
     CDS             1300..>2139
                     /locus_tag="VFL84_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET7608456.1"
                     /translation="MQPVLIGIGAYLVVQFALGMLVSRRIASESDYLLAGRRLGVTLA
                     AFSIFATWFGAETVVGAAGRIYQNGLKGGSADPFGYALCLVVLGVVVAVPLWLKQYTT
                     FGDMFRERYSPGVERLAVLLMVPTSVLWAAAQIRAFGQVVSVSSDIGVDVAITAAAAF
                     VIIYTVAGGLLADVVTDFVQSIGIVVGLAILLLAVGNAHGGLPALVASIDPERLGLFS
                     TADATPLQMLEAWAVPVCGSLLAVEMLSRILGCKTAATARNATLAGAAIYLVVGSIPV
                     LLGL"
BASE COUNT          309 a          710 c          706 g          414 t
ORIGIN      
        1 ggctcaggtc taagcttcgc ggtcgtcagc acgtcggcgg gctgatcggg atcgaagaaa
       61 ggccggccga cgcgctgcca caagcccgtc cagtccggca tgtcgtcgag cgacagcggc
      121 gcgccgccgt tcgcgcgcgc cttcaacgcg gcgtagagat cggcggccgt cgcgtgctcg
      181 agccccagcg cgattttcgc ggcacggtcg gccggcacga gctcgcccgg cgcggcactc
      241 gactccggcg gcggcgactc cggcgcgcac gcggccaacg cgagcgccgc tagaaccaag
      301 gctgaccatt tcgcgcgcac gagcggctcc taaccgacgt cagttgtcct tgtccgtgaa
      361 cgtgaaatcg cggaactgcg acgagccgtt ctcggtctgg atgacggtgt tgttctggtt
      421 ctcgccgcaa tcccagtagc ggatgcgcaa gctcttgtcg tcgttcgtga gcttcaggta
      481 cgtgtgcttc gagtaccacg gctgcgcgag cgacggcgga tcgtagaccc agacgtgcgc
      541 gagcagcgtc ttgtcgtcga ctttctccca tatctcgacg gtctcgactt gctcggtgta
      601 ttcgggctgc gtgcgctgat actgcccgct gcgcagcgag ttcgtgtgga tcacgagctt
      661 gccgccgtcc cagaaaccga tcgagtcgcc gttccagagc ggataccggt cggcttcggg
      721 cgtgtgctcg cgcccgtccg tgtagatgcg gcggatgtcg ttcatcatct cgttgatcag
      781 atacgtctga tcgggcgtca cgacgaagtc gcgcaagaac ggctccgtca tccagcgcgg
      841 atgacccggc ggcgcgcaat tgctcaggtt gtcgaactcg atgtccttct cgatgttgtc
      901 gagcttcgtg acgagccgcc gctcgaactc cggcgtcagc ttcgccgtcg gcatgcgatt
      961 gcggccttgg tcctgatccc aattgaaaag gactccgctg cgcgaataaa cgccggtcca
     1021 atcgggcaag cgcgcgggat tcaaacgctg cccgccgttc gcttgtttct cgaagtcttt
     1081 atagaggtcc cacgccgtgc gatgcttctc gccgagcgcg atcagcgcgg ctttgtcggc
     1141 cggaagctcg atcgcggcga agctcgccga ggccaccagc gtgccgagcg caacgataca
     1201 aatcttcggc accagcacag ggaacgaacg acgactaacg gttgacatgg ccttccctct
     1261 ttcgcgactc gagcacacgc taaaccgtgc cagggtgcca tgcaaccggt cctgatcggc
     1321 ataggcgcct acctcgtcgt gcagttcgcg ctcggcatgc tcgtgtcgcg gcgcatcgcg
     1381 agcgagagcg actacctgct cgcgggccgc cgcctcggcg tgacgctcgc ggcgttttcg
     1441 attttcgcga cctggttcgg cgccgagacc gtcgtcggcg cggccggcag gatctaccag
     1501 aacggcctca agggcggctc ggccgatccg ttcggctacg cgctgtgcct cgtcgttctc
     1561 ggcgtcgtcg tcgccgtgcc gctgtggctg aagcagtaca cgacgttcgg cgacatgttt
     1621 cgcgagcgct actcgcccgg cgtcgagcgg ctcgccgtgc tgttgatggt gccgacgtcg
     1681 gtgctctggg ccgccgccca aatccgggcc ttcggtcaag tcgtgagcgt gtcgtcggac
     1741 atcggagtcg acgtcgcgat cacggccgcc gccgcgttcg tcatcatcta tacggtcgcg
     1801 ggcgggctgc tcgcagacgt cgtgacggac ttcgtgcaga gcatcggcat cgtcgtgggg
     1861 ctcgcgatcc tgctgctcgc cgtcggcaac gcgcacggcg gtttgccggc gctcgtcgcg
     1921 tcgatcgatc ccgagcgtct cgggctcttc tcgacggccg acgcgacgcc gcttcagatg
     1981 ctcgaagcat gggcggtgcc ggtgtgcggc tcgctgctcg ccgtcgagat gctgtcgcgc
     2041 atcttgggct gcaagacggc ggcgacggcg cgcaacgcga cgctcgccgg cgccgcgatc
     2101 tacctcgtcg tgggctcgat tcccgtgctg ctcggtctc
//
LOCUS       DASSNN010000393         4207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_303661, whole genome shotgun sequence.
ACCESSION   DASSNN010000393 DASSNN010000000
VERSION     DASSNN010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303661"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..464)
                     /locus_tag="VFL84_07255"
     CDS             complement(135..464)
                     /locus_tag="VFL84_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET7608457.1"
                     /translation="MPAWYTTIGLVGLGVVTVLTRGFFLLSDREIELPAWAVQGLRYA
                     PIAALVAVISPEIVLTNGSLLATWHDARIYAVLVSTAYFWWRRGILGTIVSGTAVLLA
                     LKLGLGW"
     gene            complement(464..1174)
                     /locus_tag="VFL84_07260"
     CDS             complement(464..1174)
                     /locus_tag="VFL84_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET7608458.1"
                     /translation="MSLIRHPEFRRGFSETVAPSLGIAAWGLVTGVAMVKSGLSLPLA
                     VLMTMVVYAGSAQLAALPLLVAGAPLWVLWATAFCVNLRFVIYSAHWRRYFGHLPRAQ
                     RLLLGYFAVDLSYVLFARRFPEPASQREQLAYFWGSAAWNWLSWQGASLLGIVLADRV
                     PTEWGLGFAGVLALLGITYSLLSDKKTWIAAGVAAFVAVAAFALPLKLNIVAAIAAGV
                     ATGLLLDRALRNSATERA"
     gene            complement(1171..1878)
                     /locus_tag="VFL84_07265"
     CDS             complement(1171..1878)
                     /locus_tag="VFL84_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7608459.1"
                     /translation="MKILLVDDDADLLAVTGFALQQAGFLVVKAADGVAALDAYEREQ
                     PHLAVLDINMPRMSGFELAQKLRERSRIPLLMLTARSEEADVVRALALGADDYLSKPF
                     SPKILIARVKALLRRAGADATDAVAVGSLSLDASELLLLGLPRGPVRLTPLEARFLQL
                     LFAHAGRTVSTDRLLIHVWGNRAGGNRQLLKQLVHRLRQKIEDDPADPRLVRNVPNAG
                     YLLDPGAAPARAQDTQH"
     gene            complement(1875..4022)
                     /locus_tag="VFL84_07270"
     CDS             complement(1875..4022)
                     /locus_tag="VFL84_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7608460.1"
                     /translation="MTALERLKKQYESAAAVVLRRARFYAPGLAGRLALATGLLVALA
                     VAAMSGFAISALRRLADAEGLTRVELAVSSAREGMRQSTEDLLTAARTLGERPALQRQ
                     LRGPVPETLQPFLARYCESVALDACALVRGQDLITTTPGPIEWDLVLAAASEQGERFL
                     ITGAAPKMALSGAQAGVAEHAGISVIVLRNLDERLAARLTERTGVPISIIDYASFEPG
                     EGPLAILNTDALSRGGAVASYVGAIGSYAASLPVTSTSGETVALLNARLAVEEVMAPA
                     SNMARNIMVVAALIAALAIAASIFIGRYWISAVEGLTDAAERLGSGDLAASIPIGGGK
                     ELTLLGSTMERMRRNLIELTAELRRSELEAKAVLGGIVEGVYSVDEDRRIRFLNPQAE
                     RLLGISAAGATGRFCGDVLKPAVDALGRRPCEHSCPIIEARRGGSGRSLEQVAPVRPD
                     SPRRVVIASAPPSADGMQVQVLRDETELEAARRTRDTVLANISHELRTPLAAQLASIE
                     LLREGLGSMSVEGQRELVTSLHRGTQRLTWLIDNLLESVRIESGQLAIRHQDVVLEDV
                     IVAARDLIEPLIAQRGQRIEVELGEGVPVVRGDQQRLTQVVVNLLANANKFAPADSTI
                     RIGATANHSERGGLTFWVEDEGIASPGIEDARLFEQFRRSNGEGDRDESGIGLGLFIV
                     RSIVERHGGTVRLERTSDGRTRATVELPQEAPA"
     gene            complement(4019..>4207)
                     /locus_tag="VFL84_07275"
     CDS             complement(4019..>4207)
                     /locus_tag="VFL84_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608461.1"
                     /translation="VAVVSGHIRGLTTTMPLQAQVLYDEYDLVGAFAVASLLMLLALL
                     TLVLKTVIEAFTARRRRV"
BASE COUNT          703 a         1489 c         1437 g          578 t
ORIGIN      
        1 tgcggctcgt gtgcgcacgt cgccgccatg gatggcggcg tgcagcaatt gcaggagcaa
       61 attgctgccc ctgtgcgcac acggtctcgg aagggccgtg tgaagtcaac tgcgctgccg
      121 cgtcgtgacg gtcctcacca accgaggcct agcttcaggg cgaggaggac ggcggtgccg
      181 ctgacgatcg tgcctaggat gccgcggcgc caccagaaat acgcagtgct gacgaggact
      241 gcgtagatgc gcgcgtcatg ccacgtcgcg agcagcgagc cgttggtcag cacgatctcg
      301 ggcgagatca cggcgacgag cgccgcgatc ggcgcatagc gcaagccctg caccgcccac
      361 gccggcagct cgatctcacg atcggacagc aggaagaacc cgcgcgtcaa taccgtcacg
      421 acgccgaggc cgacgagccc tatcgtcgtg taccaagccg gcatcacgca cgctccgttg
      481 cggagttgcg aagcgcccgg tcgagcagca aaccggtcgc gacgccggcc gcgatcgccg
      541 caacgatgtt gagcttgagc ggcaacgcga acgcggccac ggccacgaag gccgcgacgc
      601 cggccgcgat ccaggtcttc ttgtccgaca ggagcgaata cgtgatgccg agcagcgcca
      661 gcacgccggc gaagcccaga ccccactccg tgggcacgcg atccgcgaga acgatgccga
      721 gcagcgacgc gccctgccag ctcagccaat tccaggcggc gctgccccaa aaatacgcca
      781 gctgctcgcg ctgcgatgcc ggttccggaa agcggcgcgc gaacagcacg tagctcaaat
      841 cgaccgcgaa atagccgagc agcaagcgtt gcgctcgagg caagtgcccg aagtatctgc
      901 gccaatgcgc actgtagatg acgaaccgca aatttacgca gaacgccgtc gcccacagca
      961 cccagagcgg cgcgccggcg acgaggagcg gcaacgcggc gagctgcgcg ctgcccgcgt
     1021 agacgaccat cgtcatcaag acggcgagcg gcagcgacaa gccgctcttg accatcgcga
     1081 cgcccgtgac gagcccccag gccgcgatgc cgagcgacgg cgcgaccgtc tcgctgaagc
     1141 cgcggcgaaa ctccgggtgc cggatgaggc tcaatgctgc gtgtcttgcg ctcgagccgg
     1201 cgcagcgccc gggtccaaga gatacccggc attcggcacg ttgcgcacga gccgcgggtc
     1261 ggccggatcg tcctcgatct tctggcgcag acggtgcacg agctgcttca gcagctggcg
     1321 attgccgccg gcgcggttgc cccagacgtg aatcaacagc cgatcggtcg agacggtacg
     1381 ccccgcgtgc gcaaacaaga gctgcaggaa gcgcgcctcg agcggcgtca gccgcacggg
     1441 cccgcgcggc agcccgagca gcaaaagctc ggacgcgtcg agcgacagcg agccgacggc
     1501 gacggcgtcc gtcgcgtccg ctccggcgcg ccgcaagagc gccttgacgc gcgcgatcag
     1561 gatcttcggg ctgaacggct tgctcaagta gtcgtcggcg ccgagagcga gcgcgcgcac
     1621 gacgtcggct tcctcgctgc gcgccgtgag catgagcagc ggaatgcggc tgcgctcgcg
     1681 cagcttttgc gcgagctcga agccgctcat gcgcggcatg ttgatgtcga gcacggcgag
     1741 atgcggctgc tcgcgctcgt acgcgtcgag cgccgccacg ccgtccgctg ctttgacgac
     1801 gaggaagccg gcctgctgca gcgcgaaacc ggtcacggcc agcaagtccg cgtcgtcgtc
     1861 gacgagcagt atcttcatgc cggcgcttcc tgcggcagct cgaccgtcgc gcgcgtgcgc
     1921 ccgtcgctcg tgcgctcgag ccgcacggtg ccgccgtgac gctcgacgat cgagcggacg
     1981 atgaagagcc cgaggccgat gccgctctcg tcgcgatcgc cctcgccgtt cgagcggcgg
     2041 aactgctcga acagccgcgc atcctcgatg cccggcgaag caatgccctc gtcttccacc
     2101 cagaacgtga gcccgcctcg ctccgagtgg ttggccgtgg cgccgatgcg aatcgtgctg
     2161 tcggccggcg cgaacttgtt ggcgttcgcg agcagattga cgacgacttg cgtcagacgc
     2221 tgctggtcgc cgcgcacgac gggcacgcct tccccgagct cgacctcgat gcgttgccct
     2281 cgctgcgcga tcagcggctc tatcagatcg cgcgccgcga cgatgacgtc ctcgagcaca
     2341 acgtcctgat gacgaatcgc gagctggccc gattcgatgc gcacgctctc gagcagattg
     2401 tcgatgagcc acgtgagccg ctgcgtgccg cgatgcagcg acgtgacgag ctcgcgctgg
     2461 ccctcgacgc tcatgctgcc gaggccttcg cgcagcagct cgatcgacgc gagctgcgcc
     2521 gcgagcggcg tgcgaagctc gtgcgagatg ttggcgagca ccgtgtcgcg cgtgcggcgc
     2581 gcggcctcga gctccgtctc gtcacgcagc acttgcactt gcatgccgtc ggccgagggc
     2641 ggcgcgctcg cgatgacgac gcggcgaggg ctgtccggac ggacgggcgc gacttgctcg
     2701 agcgagcggc cggagccgcc gcggcgcgct tcgatgatcg ggcacgaatg ctcgcacggc
     2761 cggcggccga gcgcgtcgac ggcgggcttc agcacgtcgc cgcagaaccg gcccgtcgcg
     2821 cccgccgccg agatcccgag caggcgctcg gcctgcgggt tcaggaagcg aatgcgccgg
     2881 tcttcgtcga cggaatagac gccctcgacg atgccgccga gcacggcctt cgcttcgagc
     2941 tcgctgcgcc gcaactcggc cgtgagctcg atgagattgc gccgcatccg ttccatcgtg
     3001 ctgccgagca gcgtgagctc cttgccgccg ccgatcggaa tcgacgcggc gaggtcgccg
     3061 gagccgagcc gctcggccgc gtccgtgagg ccctcgacgg cgctgatcca gtagcggccg
     3121 atgaagatgc tcgccgcgat ggccaacgcc gcgatcagcg ccgcgaccac catgatgttg
     3181 cgcgccatgt tgctcgccgg cgccatgact tcctctacgg cgagacgcgc gttcagcagc
     3241 gcgacggtct cgccgctcgt cgacgtgacc ggcaagctcg cggcgtacga gccgatggcg
     3301 ccgacgtacg acgccaccgc gcctccacgc gacagcgcgt ccgtgttcaa gatcgcgagc
     3361 ggcccttctc ccggctcgaa cgaagcgtag tcgatgatcg agatcggcac gcccgtgcgc
     3421 tcggtgagcc gcgccgcgag ccgttcgtcc agattgcgca gcacgatgac ggagatgccg
     3481 gcatgctcgg ccacgccggc ctgcgcgccc gacaacgcca tcttcggcgc cgcaccggtg
     3541 atcaggaacc gctcgccctg ctcgcttgcc gccgcgagca cgaggtccca ctcgatcggc
     3601 cccggcgtcg tcgtgatcaa gtcctggccc cgcacgagcg cgcaggcgtc gagtgcgacg
     3661 ctctcgcaat agcgggcgag gaacggctgc agcgtctcgg gcacgggacc gcgcagctgc
     3721 cgctgcagtg cgggccgctc gcccaacgtg cgcgcggccg tcagcaagtc ctcggtgctc
     3781 tggcgcatgc cttcgcgagc ggacgagacc gccagctcga cgcgcgtgag gccctcggca
     3841 tcggcaagcc ggcgcagcgc gctgatcgcg aaccccgaca tcgcggcgac ggccagcgcg
     3901 accagcaagc ccgtcgcgag cgcgagccgg cccgcgaggc cgggcgcgta aaatcgggcc
     3961 cgccgcagca cgacggccgc ggcggactcg tactgtttct tcaggcgctc gagcgcagtc
     4021 atacgcggcg gcgccgtgcg gtgaacgcct cgatgacggt tttgagcacc aacgtcagca
     4081 acgcgagcag catgagtagc gaggcgacgg caaaagcgcc gacgagatca tactcgtcgt
     4141 agagcacctg cgcctgcagc ggcatcgtcg tcgtgagccc gcgaatgtgc ccggaaacga
     4201 cggccac
//
LOCUS       DASSNN010000394         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_198674, whole genome shotgun sequence.
ACCESSION   DASSNN010000394 DASSNN010000000
VERSION     DASSNN010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198674"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VFL84_07280"
     CDS             <1..761
                     /locus_tag="VFL84_07280"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET7608462.1"
                     /translation="FVTQSHQPTENGAVRPDMVIRLPEERDLVVDVKTPLDAYLDATE
                     AATDAERRVALERHAAIVAGRIRELAAKAYWSQFERSPEFVILFIPGDQFLSAALAER
                     PTLLDDALRQNIILATPTSLVALLKAVAYGWQQTALAQNAAEIRTSALQLYERLATFA
                     SHLGNVGKSLADSVRAFNASVGSLERMVLPSARRFTDLGVQPRQRVVPPKPIEELTRD
                     VSAVDAPLSAVHVPVSAVDAPATTSPGEGPQLTS"
     gene            758..1540
                     /locus_tag="VFL84_07285"
     CDS             758..1540
                     /locus_tag="VFL84_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HET7608463.1"
                     /translation="MTAAETTVDWRSYRPAPQDLDGRVIMVTGATSGIGRAVARGLVV
                     HGATVILHGRNEKALEALYQELKPLGPEPSVAQIDLERAQGPQYQQLLDEIEARYGRL
                     DGLLHNAAILGDRAPIEHYDIGLWQRVLLVDLTAPFILTRCLLPLMRNSADASLLFAS
                     SSVGRRGRAHWGAYSVAKGGIENLAEILTDELENTAIRVNVINPGGTRTKMRMRAYPA
                     ENAAKLPTPESVAPPYLYLLGGASRAIRGRRFEVRDGSAAPL"
     gene            complement(1537..2154)
                     /locus_tag="VFL84_07290"
     CDS             complement(1537..2154)
                     /locus_tag="VFL84_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608464.1"
                     /translation="MSRGLLSWTHLYIVAIALASAGPLLGQAAGRQLLNSERIQANFG
                     TYGIEVLEQGERVRVSNLYSFPRAGDRTCRTFAVVQYAASIDPAIKAEHAAIVAGGSI
                     GAVFAAGGWEVRKRNLSYDEIPATPKLATLMRVAANTPLAEHVYVLDVVKGARTIEYA
                     TVAEIHHPSYLRVVDLVKIYGPVNAEQSRAPSVSAMRELAAQRAR"
BASE COUNT          404 a          807 c          794 g          384 t
ORIGIN      
        1 acttcgtcac gcagagccat cagccgacgg agaacggcgc cgttcggccc gacatggtca
       61 ttcgcttgcc cgaggagcgc gacctcgtcg tcgacgtcaa gacgccgctc gatgcctatc
      121 tcgatgcgac cgaggccgcg accgacgccg agcgccgcgt cgcgctcgag cggcacgcgg
      181 cgatcgtcgc cgggcgcatc cgcgagctcg ccgccaaagc gtactggagc cagttcgagc
      241 gcagccccga gttcgtgatc ctgttcattc cgggcgatca gttcctgagc gcggcgctcg
      301 ccgagcgccc cacgctgctc gacgacgcgc tgcgccagaa catcatcctc gcgacgccga
      361 cgagcctcgt cgcgctgttg aaggcggttg cttacggttg gcagcagacc gcgctcgcgc
      421 agaatgcggc ggagattcgc acgtcggcgc tgcagctcta cgagcgtctc gcgacgttcg
      481 cgagtcactt gggcaacgtc ggcaagtcgc tcgccgacag cgtgcgcgcc ttcaacgcga
      541 gcgtcggctc gctcgagcgc atggtgttgc cgagcgcgcg ccgctttacc gatctcggcg
      601 tgcagccgcg gcaacgcgtc gtgccgccga agccgatcga ggagctgacg cgcgacgtct
      661 cggccgtcga cgctccgctc tccgccgtcc acgttcccgt ctccgcagtc gacgctcctg
      721 cgacgacgag ccccggcgaa ggaccgcaac tcacttcatg acagccgcag aaacaacggt
      781 cgattggcgc agctaccgcc cggcgccgca ggatctggac gggcgcgtga tcatggtgac
      841 gggcgccacg tccggaatcg gccgggccgt cgcgcgcggg ctcgtcgtgc acggcgcgac
      901 ggtcattctg cacgggcgca acgagaaggc gctcgaggcg ctgtaccagg agctgaagcc
      961 gctcggcccc gagccgtcgg tcgcgcagat cgacctcgag cgcgcgcagg gaccgcagta
     1021 tcagcagctc ttggacgaga tcgaggcgcg ctacggccgg ctcgacggcc tgctgcacaa
     1081 cgccgcgatc ctcggcgacc gcgcacctat cgagcactac gacatcgggc tctggcagcg
     1141 cgtgctgctc gtcgacctga cggcgccgtt cattctgacg cgctgcctgc tgccgctcat
     1201 gcgcaactcc gccgacgcgt ctttgctgtt cgcgtcgagc agcgtcggcc gccgcggccg
     1261 cgcgcattgg ggcgcgtact ccgtcgcgaa aggcggcatc gagaatctcg ccgagatctt
     1321 gacggacgag ctcgagaaca ccgcgattcg cgtcaacgtc atcaacccgg gcggcacgcg
     1381 gacgaaaatg cgcatgcgcg cgtatccggc cgagaatgcg gcgaagctgc cgacgcccga
     1441 aagcgtcgcg ccgccgtatc tttatctgct cggcggcgcg agccgcgcga tccgcggccg
     1501 gcgcttcgag gttcgcgacg gcagcgcggc gccgctttaa cgcgcgcgtt gtgccgcgag
     1561 ctcgcgcatc gcgctgacgg agggcgcgcg actttgctcg gcgttgacgg gtccgtagat
     1621 cttcacgagg tcgacgacgc gcagatacga cgggtgatga atctcggcga ccgtcgcata
     1681 ttcgatcgtg cgcgcgccct tgacgacgtc gaggacatag acgtgctccg cgagcggcgt
     1741 gttcgcggcg acgcgcatca acgttgcgag cttcggcgta gcggggatct cgtcgtagct
     1801 cagatttcgc ttgcgaacct cccagccgcc ggccgcaaac acggcgccga tggacccgcc
     1861 ggcgacgatc gccgcgtgct cggctttgat cgcgggatcg atgctcgcgg cgtattgcac
     1921 gacggcgaaa gtccggcacg tccggtcgcc ggcgcgcgga aagctgtaca ggttcgagac
     1981 gcgcacgcgc tcgccttgct cgagcacctc gatgccgtag gtgccgaagt tggcctgaat
     2041 ccgctcgctg ttcaggagct gccggccggc ggcctgtccc agcagcggcc ctgcggacgc
     2101 gagcgcaatc gcgacaatat atagatgtgt ccacgagaga agaccccggg acatggaatg
     2161 gcgttggtat gcggtgtgca tacccaagga caacaagagt gagtgcatgg ctggagaaac
     2221 cttaattcgg ccgtgtcaat caaaaaaccc tgcggtatat actaagttcg aaagcaaccg
     2281 ttcatcgagc caacggggct atcgcgccca gattcctcct taaacggtgc agcgccaatc
     2341 aactggggag gtctcgtggg caggaaactg tacgtcggaa atttgccgt
//
LOCUS       DASSNN010000395         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1817569, whole genome shotgun sequence.
ACCESSION   DASSNN010000395 DASSNN010000000
VERSION     DASSNN010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817569"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFL84_07295"
     CDS             <1..500
                     /locus_tag="VFL84_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016856050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HET7608465.1"
                     /translation="EVAAAGCTTFVVHARKAWLSGLSPKENREVPPLDYARVYRLKRE
                     LPGLKVVINGGFVALEDSVAQLDHVDGVMVGRAAYQNSWLLARLDERLYGGPAVDEAE
                     ILEAFERYVARELASGTPLRAMTRHLLGMRSGRAGGRRWRRDLSLLGEGPRGLENLRA
                     LVAAF"
     gene            519..1340
                     /locus_tag="VFL84_07300"
     CDS             519..1340
                     /locus_tag="VFL84_07300"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7608466.1"
                     /translation="MAATAKKKLSATMAEKSDIHDLYEQSVQAVDVEAEFLRDTYRAL
                     RGRDASSLREDFCGTASLSCEWVRTGPRRHAIGVDIDAGVLDWGRRKRVARLPEAARA
                     RVELLNDDVRTVSTQPVDIVGAFNFSYFCFKTRDEMRGYFARVRQALSPGGLFFLDAF
                     GGPDASDLCKEATKIDGFTYIWEQAEFEPVTSRILCHIHFKFPDGSKIKRAFTYDWRL
                     WTLPELRELLEEAGFSKSRVYWEGDDGDGDGNGVFTEHATGEADLAWIAYIVAEK"
     gene            1418..>2115
                     /locus_tag="VFL84_07305"
     CDS             1418..>2115
                     /locus_tag="VFL84_07305"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7608467.1"
                     /translation="MGKDVEVAILFADVVGSTQLYEQLGDAKARQMVARCLDIMREAT
                     EANRGTVIKTMGDEVMSTFPTADDAMNAAKRMQERISGDIQLDHDGGHVSIRIGCHFG
                     PVVQEQRDIFGSAVHTANRMTSQAKAKQIITTLATVQKLSPEWQAATRQIDIATVRGK
                     ADEVVLFEVLWQPEDATSMLPTVGQGRNKGTRPKRLSLRYQGREVTVGEGRKSATLGR
                     AEDNDVVVKGNLISR"
BASE COUNT          368 a          703 c          726 g          318 t
ORIGIN      
        1 gcgaggtcgc cgcggccggc tgcacgacgt tcgtcgtgca cgcgcgcaag gcgtggctca
       61 gcggcttgag cccgaaagag aaccgcgagg ttccgccgct cgattacgcg cgcgtgtacc
      121 gcttgaagcg cgagctcccc ggcttgaagg tagtcatcaa cggcgggttc gtggcgctcg
      181 aggacagtgt cgcgcagctc gaccacgtcg acggggtcat ggtcggccgg gccgcgtatc
      241 agaactcgtg gctgctcgcg cggctcgacg agcggttgta cggcggcccc gccgtcgacg
      301 aggccgagat cctcgaagcg ttcgagcgat acgtcgcgcg cgagctcgcc tcgggcacgc
      361 cgttgcgcgc gatgacgcgc cacctgctcg gcatgcggag cggccgagcg ggcgggcggc
      421 gctggcgccg ggacttgagc ctcctcggcg aaggcccgcg cggcctcgag aacctgcgcg
      481 cgctcgtcgc cgctttctaa ctcgacgagg tagacgacgt ggcagccacg gcgaagaaga
      541 agctttcggc cacgatggcc gaaaaatccg acatccacga tctctacgag cagtccgtgc
      601 aggccgtcga cgtcgaggcc gagttcttgc gcgacacgta ccgcgcgctg cgcggccgcg
      661 acgcttcgag cctgcgcgaa gatttttgcg gcaccgcgag cctctcctgc gagtgggtgc
      721 gaaccggccc gcggcgccat gcgatcggcg tcgacatcga cgccggcgtg ctcgattggg
      781 gacgccgcaa gcgcgtcgcg cggttgcccg aggcggcgcg cgcgcgcgtc gagctcttga
      841 acgacgacgt gcgcaccgtg agcacgcagc ccgtcgacat cgtcggcgcg ttcaacttca
      901 gctacttctg cttcaagacg cgcgacgaga tgcgcggcta tttcgctcgc gtgcgccagg
      961 cgctgagccc gggcgggctc ttcttcctcg acgcgttcgg cggtcccgat gcctcggacc
     1021 tctgcaagga agcgaccaag atcgacggct tcacgtacat ctgggagcaa gcggagttcg
     1081 agcccgtgac gagccgcatc ctctgccaca tccatttcaa gttccccgac ggctcgaaga
     1141 tcaagcgcgc tttcacgtac gactggcggc tctggacgct ccccgagctg cgcgagctgc
     1201 tcgaagaggc cgggttctcg aagagccgcg tctactggga aggcgacgac ggcgacggcg
     1261 acggcaacgg cgtgttcacg gagcatgcga ccggcgaggc cgatctcgct tggatcgctt
     1321 acatcgtcgc cgaaaagtga cgcgacccaa gtcccattgg ccgcgctgtt cctagcgtat
     1381 aatcgcctcg gctccccgct gcgcacgaaa ggaagccatg ggtaaggatg tagaagttgc
     1441 gattctgttc gccgacgtgg tgggcagcac gcagctatac gaacagctcg gcgatgcgaa
     1501 ggcgcgccag atggtcgctc gctgcctcga catcatgcgc gaagcgaccg aggccaatcg
     1561 cggcaccgtc atcaagacga tgggcgacga ggtcatgtcg acgtttccga cggccgacga
     1621 cgccatgaac gccgcgaagc gcatgcagga gcgcatctcc ggcgacattc agctcgacca
     1681 cgacggcggc cacgtgtcga tccgcatcgg ctgccacttc ggccccgtcg tgcaggagca
     1741 gcgcgacatc ttcggctcgg cggtgcacac ggcgaaccgc atgacgagcc aggcgaaggc
     1801 caagcagatc atcaccacgc tggccaccgt gcagaagctc tcgccggagt ggcaggccgc
     1861 cacgcggcag atcgacatcg cgacggtgcg cggcaaggcc gacgaggtcg tgctgttcga
     1921 ggtgctctgg cagcccgagg acgccacgag catgctgccg accgtcgggc agggccggaa
     1981 caagggcacg cggccgaaac gcttgagcct ccgctatcag gggcgcgagg tcacggtcgg
     2041 cgagggtcgc aagagcgcca cgctcggccg tgccgaggac aacgacgtcg tcgtcaaggg
     2101 caacttgatc tcacg
//
LOCUS       DASSNN010000396         6778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1200998, whole genome shotgun sequence.
ACCESSION   DASSNN010000396 DASSNN010000000
VERSION     DASSNN010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200998"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..193)
                     /gene="ffs"
                     /locus_tag="VFL84_07310"
     ncRNA           complement(96..193)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFL84_07310"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            319..411
                     /locus_tag="VFL84_07315"
     tRNA            319..411
                     /locus_tag="VFL84_07315"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:353..355,aa:Ser,seq:gga)
     gene            484..1440
                     /locus_tag="VFL84_07320"
     CDS             484..1440
                     /locus_tag="VFL84_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HET7608468.1"
                     /translation="MADKKIIAVLGSTGSQGGGLARAILADPAGGFAARAVTRDVNKD
                     KAKALKSAGAEVVKADLDDVESLKKAFAGAYGVYAVTNFWEHFSGEKEKAQAKNVADA
                     AKAAGVKHVIWSTLEDIRKFMKPDDKRMPMLQQKYRVPHFDAKAEANASFSGLPTTFL
                     VTSFYWDNLYMFGLAPKKGEDGKLAWTFPMGNAKMAGIAAEDIGKVAYGIFKAGKEYI
                     GKTVGVMGEALTIDEMGKTLSKVMGTGPIQYNAVEADVYRSFGFPGADEMGNMFQAYR
                     DFAKEMLANRSVDTARKLNPSLQSFEQFVTKQKAAIKTAMGV"
     gene            complement(1566..2483)
                     /locus_tag="VFL84_07325"
     CDS             complement(1566..2483)
                     /locus_tag="VFL84_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7608469.1"
                     /translation="MSLRDRTAILAMLVVGALASSNVDAQTAAQDPSATTASVLWQPS
                     MNVFRRFEVPAERMYEFYGGVLGFKQLNTIAVGNAGGVARFQAGAQELKLTRRVANRS
                     YFGGGVRGATGLRLLTFFFADEAQLSARFTEHGLAAPEFKSYRGEARRSALVQDPDGQ
                     DVELVIVRDATAEQLARIEVGLTVADLARSREFYRQFVGLEELPPADDDRFGTRKYSF
                     VHGATTISLRSFGKDLAVDNGSGGIQYVVSNVDFVDALAKERNVPVEQPLSGLRGFDL
                     RTIWLGDPDGVTNYFAETGASRAAPPKAR"
     gene            2555..3916
                     /locus_tag="VFL84_07330"
     CDS             2555..3916
                     /locus_tag="VFL84_07330"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7608470.1"
                     /translation="MWKVLVALLVVGVGAAALWVNAGNAKGPAIEIGGPEALGQTGEV
                     AVKVTAPRGELTGLTVKLVQGEASTTLFDLGPAATNAAATVGDEVSLSIPAGKRALPD
                     LKAGEARIDVTAVRPVLFGLRQASATATRTIQVRLTPPQVAVLSQFHYVNHGGSEMVV
                     YRVTPPDAESGVRVGNIEYRGFPAGGGDASMRVAFFALLWDQDLNTPITVYARDSIGN
                     EGSGSFDFRVFPKQFRKSTINLDDRFLARVVPPILQNSTELTVADPTNFLASYLAINR
                     ELRRMNNETITSLARETAPEILWRGPFKQLINTAVEAGFADQRTYVYNGADVDHQVHL
                     GFDLASTANAPVKAANRGRVVHAGWLGIYGNCVILDHGMGLQSLYAHLSSIGVSVGQM
                     VETETELGRSGSTGLAGGDHLHFTMLLGGNAITPIDWWSSQWVQDRILRKFADAGLTP
                     PSP"
     gene            complement(3927..5225)
                     /locus_tag="VFL84_07335"
     CDS             complement(3927..5225)
                     /locus_tag="VFL84_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET7608471.1"
                     /translation="MLLQIVVILVLISCNALLAMSELAVVSSRRSRLQALIKRRHRGA
                     RIAMRLLENPTDFLSTVQVGITFVGMFAAAYSGATVAEEIGGWLDRWPWISPYGQPAA
                     IAVVTLGVTYVSVVFGELLPKRIALNDPERIASALAPVMRGVARVLAPAVWVARSSAD
                     GFLRLFGLARDRKLTVTEDEVRSLVAEGTRAGVFMPKEREMIEGVLRLADRTARAIMT
                     PRTEVAWLEVNATPADIVAQLDARRLSRYPVCRETIDNPVGTVHMKNVARASLAGQPI
                     ALAEIMTQPHVVLDGTPVLKLLEGFRREGTHMAIVVDEYGTTQGVVTLSDILEAVAGT
                     LPEAGGEVGAATMVRRSDGSWLVDGSLGIDEFEDRLGISGLRGSRTFDTVAGYALFRL
                     GRLPAVGDTFVDRTGHYEILDMDGRRIDKLAFRPRAEEES"
     gene            complement(5304..5639)
                     /locus_tag="VFL84_07340"
     CDS             complement(5304..5639)
                     /locus_tag="VFL84_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608472.1"
                     /translation="MAPVQIPDDLSPPDESDAIRLPADPRPAASITAGECVEKPPSFF
                     GEARPFSTGAEEESRKSRREARREAKREARAEAPAAQPEAGPAGEPSTAPPERSDPAP
                     ADGDRTIEN"
     gene            complement(5755..6642)
                     /gene="dapA"
                     /locus_tag="VFL84_07345"
     CDS             complement(5755..6642)
                     /gene="dapA"
                     /locus_tag="VFL84_07345"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET7608473.1"
                     /translation="MLSGSMVAVVTPMDADGTVNYRQFANVIDFHVQSGTQFLVVAGT
                     TGESATLDHEEHIDVIAKACELAAGRIPIVAGTGSNSTAQTLKLSRAVDRLPVVAFLV
                     VTPYYNKPPQEGMRRHFCAIADAVSRPLILYNVPGRTGVDLKPETVIKLASHPNIQAI
                     KEATGELNRIRVLRDACGPGFKLLSGDDATCCEFMLQGGDGVISVTANVAAAGMRQLC
                     DAAIAGQRAAAEAIDARLQPLHHKLFSESNPIPVKWAMQRMGLIGPGIRLPLTPLAPQ
                     FHAAVLEAMTAANVRVAAA"
BASE COUNT         1177 a         2269 c         2210 g         1122 t
ORIGIN      
        1 cggctgaccg accagctcct cgaagcgccg cggccgccat ttgcgcgcca aaaccgtgta
       61 actcacgctc tctctgtctc cgcaaaataa gaggtccgcg ccagcccgac cccggcgccc
      121 aaccgaagct gctaccgctg ctcccttccg ggcctgacgg ggtttacaac tggttgtcgc
      181 gggggaaccg acgcggacca tcgctacttt agcagcttgg ctcggcccca agcgattcaa
      241 ggcctgcgcc gtttgtcacg ccctcagttc ttgagctacg ctgccggccc ctgctcgctc
      301 ggagccgacg gatttttcgg agaggtggcc gagtggttga aggcgcacgc ctggaaagtg
      361 tgtatacgcc caaaaggcgt atcgagggtt cgaatccctc cctctccgcc acgatctact
      421 taaagcaacg aaaggcgatt atgcttttcg cttttcaacc gaacgtctca tcgaggaatt
      481 gacatggcag acaagaaaat cattgcggta ttgggctcga cgggttcgca aggcggcggg
      541 ctcgcgcgcg ccatcttggc ggatcccgcc ggcggcttcg ccgcgcgcgc ggtcacgcgc
      601 gacgtcaaca aggacaaagc caaggcgctc aagtccgcgg gcgccgaggt cgtcaaggcc
      661 gacctcgacg acgtcgagag cttgaagaaa gcattcgcgg gcgcatacgg cgtgtacgcc
      721 gtcacgaatt tctgggagca cttctccggc gaaaaggaga aggcgcaggc gaagaacgtc
      781 gcggacgcgg ccaaagccgc cggcgtcaag cacgtgatct ggtccacgct cgaagacatc
      841 cgcaagttca tgaagccgga tgacaagcgc atgccgatgc tgcagcagaa gtatcgcgtc
      901 ccgcatttcg acgcgaaggc cgaggccaac gcgtcgttca gcggtctgcc gacgacgttc
      961 ctcgtcacgt cgttctattg ggacaacctc tacatgttcg gtctcgcgcc gaagaagggc
     1021 gaggacggca agctcgcgtg gacgttcccg atgggcaacg cgaagatggc cggaatcgcg
     1081 gccgaggaca tcggcaaggt cgcctacggc attttcaagg cgggcaagga gtacatcggc
     1141 aagaccgtcg gcgtcatggg cgaggccctc acgatcgacg agatgggcaa gacgctctcg
     1201 aaggtcatgg gcacgggtcc gatccagtac aacgccgtcg aggccgacgt ctatcgcagc
     1261 ttcggtttcc cgggcgccga cgagatgggc aacatgttcc aggcttaccg cgatttcgcc
     1321 aaagagatgt tggcgaaccg cagcgtagac accgctcgca agctgaaccc ttcgctgcaa
     1381 agcttcgagc aattcgtcac gaagcagaag gccgctatca agactgccat gggtgtctga
     1441 cacccgggcg accgccagcg cggccgccca gtgcttccgt tccagcggcg agctcgtgcg
     1501 agctcgccgc ttttcgtttc ggcaactgct cctgcgttgc cctacctcgg gcatccatgc
     1561 cgtcgtcagc gcgctttcgg cggcgccgcg cggctcgcgc cggtctctgc aaagtagttc
     1621 gtgacgccgt cgggatcgcc gagccagatc gtccgcaagt cgaacccgcg caagcccgac
     1681 agcggctgct ctaccggcac gttccgctcc ttggcgagcg cgtcgacgaa gtcgacgttc
     1741 gacacgacgt attggatgcc gccgctgccg ttgtctacgg ccaagtcttt gccgaagctg
     1801 cgcaggctga tcgtcgttgc gccgtgcacg aacgagtact tgcgcgtgcc gaagcggtcg
     1861 tcgtcggcgg gcggaagctc ctcgaggccg acgaactgcc gatagaactc cctgctgcgc
     1921 gcgaggtccg cgaccgtcag gccgacctcg atgcgcgcca gttgctcggc cgtcgcatcg
     1981 cgcacgatca cgagctcgac gtcttggccg tccggatcct gaacgagcgc cgagcggcgg
     2041 gcctcgccgc gatagctctt gaactcgggg gccgcgagcc cgtgctcggt gaagcgcgcc
     2101 gaaagctgcg cttcgtcggc gaagaagaac gtcaagagcc ttaagcccgt cgcgccgcgc
     2161 acgccgccgc caaagtagct gcggttagcg acgcggcgag tcagcttgag ctcctgggcg
     2221 cccgcctgaa atcgcgcgac gccgcctgcg ttccccacgg cgatggtgtt gagctgcttg
     2281 aagccgagca cgccgccgta gaactcgtac atgcgctcgg cgggaacctc gaacctgcgg
     2341 aacacgttca tcgacggctg ccacaacacg ctcgccgtcg tcgcgctcgg atcttgcgca
     2401 gccgtctgcg cgtctacgtt gctcgaagcg agcgccccga ctacgagcat cgcgagaatc
     2461 gcggttcgat cgcgtagcga catcgtggcc tcccgacgtg tcagattatg gcgacgagtg
     2521 tgaatcacgc gggctgtcgg tacaatcgcg gcccatgtgg aaagtactcg tcgctctatt
     2581 ggttgtcggg gtcggcgccg cggcgctgtg ggtcaatgca ggaaacgcca aagggccggc
     2641 gatcgagatc ggcggtccag aggcgctcgg ccaaacgggc gaggtcgcgg tcaaggtcac
     2701 ggcgccgcgc ggcgagctga ccggtctcac cgtgaagctg gtgcaaggtg aagccagcac
     2761 gacactcttc gatctcggtc ccgcggcgac gaacgccgcg gccacggtcg gcgacgaggt
     2821 cagcctcagc attccggccg gcaaacgcgc cctgcccgat ctcaaagccg gcgaagcgcg
     2881 aatcgacgtg acggccgtgc gccccgtgct gttcgggctg cgccaggcct ccgcaacggc
     2941 cacgcgcacg atccaagtgc gcctcacgcc gccgcaagtc gcggtgctgt cgcagttcca
     3001 ctacgtcaac cacggcggct cggagatggt cgtctatcgc gtcaccccgc ccgacgccga
     3061 atccggcgtg cgcgtcggca acatcgagta tcgcggcttc ccggcgggcg gcggcgatgc
     3121 ttcaatgcgc gtcgcgttct tcgcgctgct ctgggatcag gacttgaata ccccgatcac
     3181 ggtctacgcg cgcgactcga tcggcaacga gggcagcggc agcttcgact tccgtgtgtt
     3241 ccctaagcag tttcgcaaga gcacgatcaa tctcgacgac cggttcctcg cgcgcgtcgt
     3301 gccgccgatc ttgcagaact cgacggagct cacggtcgcc gatccgacga acttcctcgc
     3361 gtcgtacctc gcgatcaacc gcgagctgcg gcgcatgaac aacgagacga tcacgagcct
     3421 cgcgcgcgag accgcgcccg agatcttgtg gcgcggtccg ttcaagcagc tcatcaacac
     3481 ggcggtcgag gctgggttcg ccgatcaacg cacgtacgtc tacaacggcg cggacgtaga
     3541 ccatcaggtg catctcggtt tcgatctcgc gtcgacggcg aatgcgccgg tcaaagccgc
     3601 gaatcgcggc cgagtcgtgc acgccggttg gctcggcatc tacggcaact gcgtcatcct
     3661 cgatcacggc atggggctgc aatcgctgta tgcgcacttg tcctcgatcg gcgtcagcgt
     3721 cggccaaatg gtcgaaaccg agaccgagct cggacgcagc ggctcgaccg ggctcgccgg
     3781 cggcgatcac ctgcacttca cgatgctgct cggcggcaat gcgatcacgc cgatcgattg
     3841 gtggagctcg cagtgggtgc aagaccgcat cttgcgcaag ttcgccgacg cgggcctgac
     3901 gccgccgagc ccttgagcgc gtttcgctaa gactcctcct ccgcgcgcgg ccggaaggcc
     3961 agcttgtcga ttcgccggcc gtccatgtcg aggatctcgt aatggcccgt gcggtcgacg
     4021 aacgtgtcgc cgacggccgg caagcgtccg agacgaaaca gcgcgtaacc cgcgaccgtg
     4081 tcgaacgtcc gcgagccgcg caggccggaa atgccgagcc ggtcctcgaa ctcatcgatg
     4141 cccaagctgc cgtcgacgag ccatgaccca tcgctgcgcc tgaccatcgt cgcggctccg
     4201 acctcgccgc cggcttccgg taacgtgccg gccactgctt cgaggatgtc cgacagcgtc
     4261 acgacgccct gggtcgtccc gtactcgtcg acgacgatcg ccatgtgagt gccttcgcgg
     4321 cggaagcctt cgagcagctt cagcaccggc gttccgtcga gcacgacgtg cggctgagtc
     4381 atgatctcgg cgagcgcgat cggttgaccc gcgagcgagg cacgcgcgac gttcttcatg
     4441 tgcacggtgc cgacgggatt gtcgatcgtc tcgcggcata cgggatagcg cgagagcctg
     4501 cgcgcgtcga gctgcgcgac gatgtcggcc ggcgtcgcgt tgacctcgag ccacgcgact
     4561 tcggtgcgcg gcgtcatgat cgcccgcgcg gtgcgatcgg cgaggcgcaa cacgccttcg
     4621 atcatctcgc gctctttcgg catgaacacg ccggcgcgcg tgccttcggc gacgagactt
     4681 cgcacctcgt cctcggtcac ggtcagcttg cggtcgcgcg ccagcccgaa cagccgcaag
     4741 aacccgtcgg ccgaggaccg cgcgacccag accgccgggg cgagcactct cgcgaccccg
     4801 cgcatgaccg gggccagcgc ggaagcgatg cgctccggat cgttgagcgc gattcgcttt
     4861 ggcaacagct cgccgaacac gaccgacacg taagtgacgc cgagcgtcac gacggcgatc
     4921 gcggcgggct ggccgtacgg cgagatccag ggccagcggt cgagccatcc gccgatttcc
     4981 tcggccaccg tcgcgccgct gtatgcggcc gcgaacatgc cgacgaaggt gatgcctact
     5041 tggaccgtcg acaggaaatc ggtcgggttt tccagcagcc gcatcgcgat gcgcgcgccg
     5101 cggtgccggc gcttgatgag cgcctgcagc cggctgcgcc gcgacgagac gacggcgagc
     5161 tccgacattg cgagcagcgc gttgcaggag atcaaaacga ggatgacgac gatttgcaaa
     5221 agcatgaagg agcacccttg gcgctgccga attgcagcgc aggcgcttga gcgcgtgtta
     5281 gcagcctagc agacgggaaa ccgtcagttc tcgatggtgc gatcgccgtc ggccggcgcc
     5341 ggatcgctgc gctcgggcgg cgccgtgctc ggttccccgg ccgggccggc ctccggctgc
     5401 gcggccggcg cttcggcgcg cgcttctctc ttcgcttcgc ggcgcgcctc gcgacgcgac
     5461 ttgcggctct cttcctcggc gcccgtcgag aacggacgcg cttcgccgaa gaacgacggc
     5521 ggcttctcca cgcactctcc ggccgtgatc gacgcggccg gtcgcggatc ggccggcagg
     5581 cggatcgcat cactctcgtc gggcggcgac agatcgtcgg gaatctgaac cggagccacc
     5641 gagcgtgccg tcgaatagcg cggctcggga tcgcaaggcc ccgcttgctg gcgtgagcac
     5701 cccgcgacag ccgcaatccc gacgatcgcg caccaggcgc gaaggaggcg catgttaagc
     5761 ggccgcgacg cgaacgtttg cagccgtcat cgcctcgagc acagcggcat gaaattgggg
     5821 cgcgagcggc gtcagcggca agcgaatgcc ggggccgatc aggcccatgc gctgcatggc
     5881 ccacttgacc gggatcggat tgctctcgga gaacagcttg tgatggagcg gctgtaaacg
     5941 tgcgtcgatc gcttcggccg cggcgcgctg gcccgcaatc gcggcgtcgc acagctgccg
     6001 catgccggcg gctgcgacgt tagcggtcac ggaaatcacg ccatccccac cttgcaacat
     6061 gaactcgcag cacgtcgcat cgtcgccgct cagcagcttg aagcccgggc cgcatgcatc
     6121 tctgagcacg cggatccgat tcagttcccc ggtcgcttcc ttgatggcct ggatgttcgg
     6181 atgcgacgcc agcttgatga cggtctccgg cttcaggtcg acgcccgtgc ggcccggcac
     6241 gttgtagaga atcagcggcc ggctgacggc gtcggcgatc gcgcagaagt gccgccgcat
     6301 gccttcctgc ggcggcttgt tgtagtaagg cgtgacgacg agaaatgcca cgaccgggag
     6361 ccggtcgacg gcgcgcgaca gcttcagcgt ctgcgcggtc gagttcgagc cggtgccggc
     6421 cacgatcgga atacggcccg ccgcgagctc gcacgctttc gcgatgacgt cgatgtgctc
     6481 ttcgtgatcg agcgtcgccg attcgccggt ggtccccgcg acgacgagaa attgcgttcc
     6541 cgactggacg tggaaatcga tgacgttcgc aaactgccgg taattgaccg tgccgtctgc
     6601 gtccatcggc gtgacgaccg ccaccatgct gccggacaac atcatcggac cttcgcgcac
     6661 cgagccaaaa aggcgaacga tagtaccggc gccacttggg ctccgcaact tgcggcgcgg
     6721 cccgaaaagg ggacagatct attttccgcg cgtgagtggg tcggtctccg gcacgtac
//
LOCUS       DASSNN010000397         4106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1818071, whole genome shotgun sequence.
ACCESSION   DASSNN010000397 DASSNN010000000
VERSION     DASSNN010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818071"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFL84_07350"
     CDS             complement(<1..437)
                     /locus_tag="VFL84_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET7608474.1"
                     /translation="MSLGPLMIDLRGYALAADEREWLQSPLVGGVILFTRNFESVEQL
                     TRLVAEIHAARQPPLLVTVDQEGGRVQRFRAPFFRLPPLRAMGRLHDEERELALKAVA
                     ACGWLMASELRAVGIDLSFAPCVDLDRGLAKVIGDRALHREGAV"
     gene            complement(456..929)
                     /locus_tag="VFL84_07355"
     CDS             complement(456..929)
                     /locus_tag="VFL84_07355"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET7608475.1"
                     /translation="MSLRVSLAAQTLALVRGGAVAKTYSVSTSKHGAGEINGSFKTPR
                     GRHIVRAKIGAGAPLNAVFRGRRPTGEIYSAELARAEPDRDWILTRILWLSGSEIGRN
                     RLGPVDTMRRYIYIHGAPDTEPMGSPSSIGCIRMANRDVAELFELVPVGTVVDIA"
     gene            complement(926..1411)
                     /locus_tag="VFL84_07360"
     CDS             complement(926..1411)
                     /locus_tag="VFL84_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020559344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET7608476.1"
                     /translation="MPREIERKFLPKSDAWRSQVARSRTMSQGYLASGADVSVRVRVA
                     GHEAWLNIKAGGPVPSRPEYEYSIPLDEARELLAFAQKPLVEKTRHFVEHDGMTWEVD
                     EFHGDNLGLVVAELELDSEDAPFSRPEWLGVEVTHLHRYYNVCLVTHPYRAWNEAERN
                     P"
     gene            complement(1440..2741)
                     /gene="rlmD"
                     /locus_tag="VFL84_07365"
     CDS             complement(1440..2741)
                     /gene="rlmD"
                     /locus_tag="VFL84_07365"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET7608477.1"
                     /translation="MKHDAPPVVATIVDLTHDGVGVADLDGRRVFVADALPGERVEIA
                     LRKRRRKMQEADLVRVLEPSPSRVQPACEYFGRCGGCALQHLAPEAQVPFKQQVVAEA
                     LKRIGGVEPMTWLPAAVSPPWHYRRRARLGVKHVAAKHRVLVGFRERAGPYITDMLRC
                     PVLKPPLDGLLGALSELIEKSSLKERLPQIEAAVGDGVVALVFRVLADPTDADTEAFR
                     AFGARHAIDMYWQRGGLGSIVPLDRARPLTYRLGELGLTFEFLPTDFVQVNAAINAEL
                     VSTAVRLAAVEPTDRVLDLYCGLGNFSLPLAQRSSALLGVEGDAGLVARALRNASLNG
                     IHNARFVTADLMAPGWGFYRERWDVVVLDPPRTGAETPVADLDSSGTRRIVYVSCHPA
                     TLARDARLLVEKHRFKLHTARVFDMFPHTHHVEALALFTRD"
     gene            complement(2731..3516)
                     /locus_tag="VFL84_07370"
     CDS             complement(2731..3516)
                     /locus_tag="VFL84_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET7608478.1"
                     /translation="MNVLAFDIETVPDVELGRRLYELDGLDDAAVAQAMFAKQRSLKP
                     GDFLPPPQQRVVAISVVLRTRDGLKIFSLGDEQSGERELVQRFFDGLERYTPVLVSWN
                     GSGFDLPVLTYRALRHGVDAHHYWEVGESDRDFRYNNYLSRYHWRHIDLMDVLSGYGA
                     SGRASLDLASQLIGLPGKLGIGGAQVWPAYRRGELAAIREYCEADVLSTYLLFLRFQL
                     VRGELDAASYNNELAVVETKLAQSDRPHLRQYLALWQKSRHEA"
     gene            complement(3513..3908)
                     /gene="acpS"
                     /locus_tag="VFL84_07375"
     CDS             complement(3513..3908)
                     /gene="acpS"
                     /locus_tag="VFL84_07375"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET7608479.1"
                     /translation="MIFGIGTDVLQAERVEKTWQRFGEHFARRLLLDEELELFAKAKR
                     PVRFLAMRFAAKEAIVKALGTGFANGIWVRDVGMMPNALGQPQVIYSERGLALCRKLG
                     VGEGHLTLSDEAGLIVAVAVLMRASSEKA"
     gene            complement(3905..>4106)
                     /locus_tag="VFL84_07380"
     CDS             complement(3905..>4106)
                     /locus_tag="VFL84_07380"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014603231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HET7608480.1"
                     /translation="GVARARALGLVVHAGHGLNYHNVAPIAAMRSIVELNIGHAIVSQ
                     ALFDGFAQAVAKMKALMVAARA"
BASE COUNT          697 a         1413 c         1375 g          621 t
ORIGIN      
        1 acggcgcctt cgcgatgcag cgcgcgatcg ccaatgacct tcgcgaggcc gcggtcgaga
       61 tcgacgcacg gcgcgaagct caagtcgatg ccgacggcgc gaagctcgga ggccatgagc
      121 cagccgcaag cggcgacggc cttcagcgcg agctcgcgct cctcgtcgtg gagcctgccc
      181 attgcgcgga gcggcggcaa gcgaaagaac ggcgcgcgga atcgctgcac gcgcccgcct
      241 tcctggtcga cggtcacgag caacggcggc tgccgcgccg cgtggatctc ggcgacgagc
      301 cgcgtaagct gctcgacgct ctcgaagttg cgcgtgaaga ggatgactcc gccgacgagc
      361 ggcgactgaa gccactcgcg ctcgtccgcg gcgagagcat atccccgcaa gtcgatcatc
      421 agtgggccca gcgacatggc ttctcttacg ctacctcacg cgatgtccac gaccgttccg
      481 acgggaacga gctcgaacag ctccgcgacg tcgcggttcg ccattcgaat gcagccgatc
      541 gagctcgggc tccccatcgg ctccgtatcc ggcgcgccat ggatatagat gtagcggcgc
      601 attgtatcga cgggcccgag ccgattcctg ccgatttcgc taccggagag ccacaaaatc
      661 cgcgtcagta tccagtcgcg gtccggctcc gcgcgcgcga gctcggccga gtagatctcg
      721 cccgtcggcc gccggccgcg aaacacggcg ttcaacggcg cgccggcgcc gattttcgcg
      781 cgtacgatgt gccggccgcg cggcgtcttg aagctgccgt tgatctcgcc ggcgccgtgc
      841 ttcgacgtcg agacgctgta agtcttcgcg acggctccgc cgcgcacgag cgcaagcgtt
      901 tgcgccgcca gcgacacacg caagctcacg ggttgcgttc ggcttcgttc catgcgcgat
      961 agggatgcgt gacgagacag acgttgtaat agcggtgcag gtgcgtgacc tcgacgccga
     1021 gccactccgg ccgcgagaac ggcgcgtcct cgctgtcgag ctcgagctcc gcgacgacta
     1081 ggcctaagtt gtcgccgtgg aactcgtcga cttcccaggt catgccgtcg tgctcgacga
     1141 aatgccgcgt cttctcgacg agcggctttt gcgcgaacgc aagcagctcg cgcgcctcgt
     1201 cgagcgggat cgaatactcg tactcggggc gcgacgggac tggaccgccc gctttgatgt
     1261 tgagccacgc ttcgtgtccc gcgacgcgca cgcgcaccga gacgtcggcg ccgctcgcga
     1321 gatagccctg gctcatcgtc cggctccgcg caacttgcga gcgccacgca tcgctcttcg
     1381 gcaggaactt gcgctcgatc tcacgcggca tgggcgcgat gatagccgcg atcgcgaagc
     1441 tagtcgcgcg tgaacagggc gagcgcctcg acgtgatgcg tgtgcgggaa catgtcgaag
     1501 acgcgggccg tgtgcagctt gaagcgatgc ttttcgacga gcaagcgcgc gtcgcgggcg
     1561 agcgtcgccg gatggcaaga gacgtagacg atgcggcgcg tgccgctcga gtccaagtct
     1621 gcgacagggg tctcggcgcc ggtgcgcggc gggtcgagca cgacgacgtc ccagcgctcg
     1681 cgatagaagc cccagccggg cgccatgaga tccgccgtca cgaagcgcgc gttgtggatg
     1741 ccgttcagcg aggcgttgcg caaggcgcgc gcgacgagcc cggcgtcgcc ttcgacgccg
     1801 agcagcgcgc tcgatcgctg cgccagcggc aagctgaagt tgccgagccc gcagtacaaa
     1861 tcgagcacgc gatccgtcgg ctcgaccgcg gccaagcgca cggccgtcga tacgagctcg
     1921 gcgttgatcg cggcgttgac ctgcacgaaa tccgtcggca ggaactcgaa cgtgagcccg
     1981 agctcgccca accgataagt cagcggccgc gcccggtcga gcggcacgat cgatcccaag
     2041 ccgccgcgct gccaatacat gtcgatggcg tgacgcgcgc cgaaggcgcg aaacgcctcc
     2101 gtgtcggcgt cggtcgggtc ggcaagcacg cgaaagacga gggccacgac gccgtcgccg
     2161 acggccgcct cgatctgcgg cagccgttcc ttgagactgc tcttctcgat gagctccgag
     2221 agcgcgccga gcaacccgtc gaggggcggc ttcaatacgg gacagcgcag catgtccgtg
     2281 atgtagggcc ccgcacgctc gcgaaagccg acgaggacgc ggtgcttcgc ggcgacgtgc
     2341 ttgacgccga gccgcgcgcg gcggcgatag tgccacggag ggctcaccgc ggcaggcagc
     2401 cacgtcatgg gctcgacgcc gccgatccgc ttcaacgcct ccgcgacgac ctgctgcttg
     2461 aacggcactt gcgcctcggg cgcgaggtgt tgcagcgcgc agccgccgca acgcccgaag
     2521 tactcgcacg caggctgaac gcgcgaaggc gaaggctcga gcacgcgaac caggtcggcc
     2581 tcctgcatct tgcggcgccg cttgcgcagc gcgatttcga cgcgctctcc gggcagcgcg
     2641 tcggcgacga agacgcgccg gccgtcgaga tcggcgacgc ctacgccgtc atgcgttagg
     2701 tcgacgatcg tggcgacgac aggcggcgcg tcatgcttca tgccgcgatt tctgccaaag
     2761 cgcgagatac tgccgcaagt gcggccggtc ggattgcgcg agcttcgtct cgacgacggc
     2821 gagctcgttg ttgtagctcg ccgcgtcgag ctcgccgcgc acgagctgga agcgcaggaa
     2881 gaggaggtac gtgctgagga cgtcggcctc gcaatactcg cgaatcgcgg cgagctcgcc
     2941 gcggcgatac gccggccaca cttgcgcgcc gccgatcccg agcttgcccg gcaagccgat
     3001 gagctgcgac gcgagatcga gcgacgcgcg cccgctcgcg ccgtagccgg acagcacgtc
     3061 catcaagtcg atgtgccgcc agtgatagcg gctcaagtag ttgttgtagc ggaagtcgcg
     3121 gtccgactcg ccgacttccc agtagtggtg cgcgtcgacg ccgtgccgca acgcgcgata
     3181 agtcagcacc ggcaagtcga agccggagcc gttccacgac acgagcacgg gcgtatagcg
     3241 ctcgagcccg tcgaagaacc gctgcacgag ctcgcgctct cccgattgct cgtcgccgag
     3301 gctgaagatc ttgagcccgt cgcgcgtgcg cagcacgacc gagatcgcga cgacacgctg
     3361 ctgcggcggc ggcaagaaat cgccgggctt gagggaacgc tgcttcgcga acatcgcctg
     3421 cgcgaccgcc gcgtcgtcga ggccgtcgag ctcatagagc cgtctgccga gctcgacgtc
     3481 gggcaccgtt tcgatgtcga atgcgagtac gttcatgcct tctccgagct cgcgcgcatc
     3541 agcacggcga ccgcgacgat gaggcccgcc tcgtcgctga gcgtgaggtg gccttcgccg
     3601 acgccgagct tcctgcacag cgcgagcccg cgctccgagt agatgacctg cggctgaccg
     3661 agcgcgttcg gcatcatgcc gacgtcgcgc acccagatgc cgttcgcgaa gcccgtgccg
     3721 agcgccttga cgatcgcttc cttcgcggcg aaccgcatag cgagaaagcg cacgggccgc
     3781 ttagccttcg cgaacagctc gagctcctcg tcgagcagca agcggcgcgc gaaatgttcg
     3841 ccgaaacgct gccacgtctt ctcgacgcgc tccgcctgca acacgtccgt gccgataccg
     3901 aagatcacgc gcgtgccgcc accatgagcg ccttcatctt cgccaccgct tgcgcgaacc
     3961 cgtcgaacaa cgcctgcgat acgatcgcgt gcccgatatt cagctcgacg atcgagcgca
     4021 tggcggcgat cggagcgacg ttgtggtagt tgagcccatg cccggcatgc acgacgagcc
     4081 cgagcgcccg cgccctagcc acgccg
//
LOCUS       DASSNN010000398         3997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1201781, whole genome shotgun sequence.
ACCESSION   DASSNN010000398 DASSNN010000000
VERSION     DASSNN010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201781"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFL84_07385"
     CDS             <1..193
                     /locus_tag="VFL84_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509553.1"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HET7608481.1"
                     /translation="NALRLTDGAPVAAFEARAGQPGAAIGAARAAAVARGWLSLEPGT
                     LRATPVGLERLNKVLELFA"
     gene            238..1098
                     /locus_tag="VFL84_07390"
     CDS             238..1098
                     /locus_tag="VFL84_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HET7608482.1"
                     /translation="MATKTWLAGALVAAALAASCTDAGNSPPATAAASPRIYVFENGT
                     ISGLDPALFNFQREELKQLDFVNSAYLIVHPRGTLMFDSGAVPDSHFKGDGSPVVEGV
                     VTADKPVLPQLKAAGYEPRDVTYFALSHYHSDHTGNANEFANATWIVQKAERDYMFGD
                     NPQGIIQPATYDALRNAKTKILDNEDFDVFGDGSVVIMSTPGHTPGHQVLAVKLANRG
                     TVVLAGDLYHYPEERTTGRIPTFEFAPEQSKVSRARIEKFVQDNRAELWIEHDAATHA
                     ALPKSPAFVD"
     gene            1107..1841
                     /locus_tag="VFL84_07395"
     CDS             1107..1841
                     /locus_tag="VFL84_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019508009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7608483.1"
                     /translation="MATVEEHYSRVLADVYSWMYGGWDAQLKRYTEFFAARGIAPRAS
                     KRAIDLGAGCGFQAIPLGRLGFDVKAIDLDRTLLAELEAHVDAEKIETICADIVEFRR
                     HAPQSAELIVCMVDTLLHLETQDAVVRLAADVFAALEPGGAFIATFRDFTVETKELER
                     FIPVRADERTVFTCFLEFEPDTVKVHDLVHKRVDGQWDFAKSFYRKLRLPTDWVVMTL
                     RGAGFAHVETGLDRGLVVVTAKKSQA"
     gene            1929..2726
                     /locus_tag="VFL84_07400"
     CDS             1929..2726
                     /locus_tag="VFL84_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET7608484.1"
                     /translation="MLSLLLTTWVAGQGQALEMSFPAEPGLTSVSVLWSGHDVPFIPS
                     GDRWVAFVGVDLDSRPGDHAVEVTFAYADGHTRVVLEPVLVNAGQYPTTELKVEERYV
                     ELSPEDQARADREAAETSAIYDTFTPERYWSEPFEVPVRGAKDGRNFGHRRVFNSQPR
                     APHSGADLRAGTGTPIYAANRGRVVLAKELFFSGNAVYIDHGYGLFTTYLHLSKIDVK
                     VGDIVERGQQLGLAGATGRVTGPHLHWGVRLLDARVDPFSLVRLGAP"
     gene            2737..2916
                     /locus_tag="VFL84_07405"
     CDS             2737..2916
                     /locus_tag="VFL84_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HET7608485.1"
                     /translation="MSNHPGRNDPCPCGSGKKYKHCCALKKERLSIGTQLWFVLIAAM
                     LLIGAWLAVTQMEVG"
     gene            3187..3639
                     /gene="rimP"
                     /locus_tag="VFL84_07410"
     CDS             3187..3639
                     /gene="rimP"
                     /locus_tag="VFL84_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET7608486.1"
                     /translation="MAQAVDKERLFELLEPAVAAMGFELVDLDAHLGRRGLLRLFIDR
                     EGGVTLDDCQRVSEQIGAFLDVEDPLPGSYVLEVSSPGFDRRLRKLEHFMRFSGDNAK
                     IELRDAVDGRRRFTGKLVGVEGSEVLLEVDGELTRLPFGDIAVARLAP"
     gene            3658..>3997
                     /locus_tag="VFL84_07415"
     CDS             3658..>3997
                     /locus_tag="VFL84_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HET7608487.1"
                     /translation="MSKEILLVVDAVSNEKGVEKSVIFEAIEAALASATRKRYGDDID
                     VRVSINRTNGSYETFRRWKVFADDSREIEEPARELRLEDALDIDPKAEVGGFVEQPMQ
                     SVEFGRIAAQT"
BASE COUNT          665 a         1285 c         1367 g          680 t
ORIGIN      
        1 gaacgcgctg cgcctcaccg acggcgcacc cgtcgcggcg ttcgaggccc gtgccggcca
       61 gcccggggcg gcgatcggcg ccgcgcgggc ggccgccgtc gcgcgggggt ggctcagcct
      121 agagcccggc acgctgcgcg cgacgccggt gggcctcgaa cggttgaaca aggtgctcga
      181 gctgttcgcg tgacgctctt gctagactcg agcgggggct caagaggggg aaccggcatg
      241 gctacgaaaa cttggctcgc gggcgcgctc gtcgcggcgg cgctggcagc gtcgtgcacg
      301 gacgcgggca actcgccgcc ggcgacggcg gctgcgtcgc cgcgcattta tgtcttcgag
      361 aacggcacga tcagcgggct cgatcccgcg ctgttcaatt tccagcgtga ggagctcaag
      421 cagctcgatt tcgtcaattc ggcgtatctg atcgtgcacc cgcgcggcac gctgatgttc
      481 gactccggcg ccgtgcccga cagccacttc aaaggcgacg gctcgcccgt cgtcgaaggc
      541 gtcgtcaccg cggacaagcc cgtgctgccg cagctcaagg ccgcgggcta cgagccgcgc
      601 gacgtcacgt acttcgcgct gtctcactat cactccgacc acacgggcaa cgccaacgag
      661 ttcgcgaacg ccacctggat cgtgcagaag gcggagcgcg actacatgtt cggcgacaat
      721 ccgcagggca tcatccagcc ggcgacgtac gacgcgttgc gcaacgccaa gacgaaaatc
      781 ctcgacaacg aggacttcga cgtgttcggc gacggctcgg tcgtgatcat gtcgacgccg
      841 ggccacacgc ccggtcacca agtcctcgcc gtcaagctcg cgaaccgcgg caccgtcgtg
      901 ctcgccggcg atctctatca ctatcccgag gagcgcacga cgggacgcat tccgacgttc
      961 gagttcgcgc ccgagcaatc gaaggtctcg cgcgcgcgca tcgagaaatt cgtgcaggac
     1021 aaccgcgccg agctctggat cgagcacgac gccgccacgc acgccgcgct gccgaagtcg
     1081 ccggcattcg tcgactgatc ggcgccgtgg cgacggtcga agagcactac agccgagtac
     1141 tcgccgacgt ctactcgtgg atgtacggcg gctgggacgc gcagctcaag cgctacaccg
     1201 agttcttcgc ggcgcggggc atcgcgccgc gcgcgtcgaa gcgcgcgatc gacttgggcg
     1261 ccggctgcgg cttccaggca attcctctcg gccggctcgg cttcgacgtc aaagcgatcg
     1321 acctcgaccg cacgctgctc gcggagctcg aagcgcacgt cgacgccgag aagatcgaga
     1381 cgatctgcgc cgacatcgtg gaattccggc gccacgcgcc gcagagcgcc gagctcatcg
     1441 tctgcatggt ggacacgctg ctgcatctcg aaacgcagga cgcggtggtg cggctcgccg
     1501 ccgacgtgtt cgctgcgctc gagccgggcg gcgcgttcat cgcgacgttt cgcgatttca
     1561 cggtcgagac gaaggagctc gagcgcttca ttcccgttcg agcggacgag cggaccgtct
     1621 tcacgtgctt cctcgagttc gagcccgaca ccgtcaaggt ccacgacctc gtccacaagc
     1681 gcgtggacgg gcagtgggat tttgcgaaga gcttttaccg taagctgcgc ttgccgacgg
     1741 actgggtcgt catgacgctg cgcggggcgg gcttcgctca cgtcgagacg ggcctcgacc
     1801 gcggcctcgt cgtcgtgacg gcgaagaagt cgcaagccta gcgccgcgcg cttgaccgcc
     1861 ttcggatgcg gccggatgat cggaaccgcg aatcgcggcc gcgtcgtcga caaggagtca
     1921 tcgcacggat gttgtcgctg ctgcttacga cctgggttgc cgggcagggt caagcgctcg
     1981 agatgagctt tccggccgag ccgggcttga cgagcgtcag cgtgctctgg agcggccacg
     2041 acgtgccgtt catcccgagc ggcgaccgct gggtcgcgtt cgtcggagtc gacctggatt
     2101 cccggcccgg cgaccacgcc gtcgaagtca cgttcgcgta cgcggacggc cacacgcgcg
     2161 tcgtcctcga gcccgtcctc gtcaacgccg ggcagtatcc gaccacggag ctcaaggtcg
     2221 aggagcgcta cgtcgagctc agccccgagg accaggcgcg cgcggaccgc gaagcggccg
     2281 agacgagcgc gatctacgac acgttcacgc ccgagcgcta ctggagcgag ccgttcgagg
     2341 tcccggttcg cggggccaag gacggccgca acttcggcca ccggcgcgta ttcaacagcc
     2401 aaccgcgagc gccgcattcg ggcgccgact tgcgagccgg caccggcacg ccgatctatg
     2461 ccgcgaaccg cggccgcgtc gtgctcgcga aggagctctt cttcagcggc aatgccgtct
     2521 atatcgacca cggctatggg ctcttcacga cctatctgca tctctcgaag atcgacgtga
     2581 aggtcggcga catcgtcgag cgcggccagc agctcggcct ggccggcgcg accggccgcg
     2641 tcacggggcc gcacctgcat tggggcgtgc gtctgctcga tgcccgcgtc gacccgttct
     2701 cgctggttcg actgggggcc ccgtaacgga gcggccgtga gcaaccatcc cgggcgcaac
     2761 gatccctgtc cctgcggcag cggcaagaaa tacaagcatt gctgcgccct gaagaaagag
     2821 cggctctcga tcggcactca gttatggttc gtcctgatcg cggccatgct gctgataggc
     2881 gcctggctcg ccgttaccca gatggaagtg ggctaagagc cgtccgccgg caaattgctc
     2941 tacatttgcg gcagtttggc catcccgcct accgcccgcg aggcgttttc tgctaggatg
     3001 cttgcggttt ttggagcaac ggctctgaag gcaagaggcc ccgtatgggg ctttttgttt
     3061 ggtccgcctc gccgggagcg ctgaatgggc gcgccgaggg gcgcggacgc gtcgcaacgt
     3121 aatgggccgt atggcccatt tttgttggtg gcgtatgccg gttgcggtgt tgggacggtc
     3181 cgaagcatgg ctcaggcggt cgacaaagag cgtttgttcg agctgctcga gccggccgtt
     3241 gcggcaatgg gtttcgagct cgtggatctc gatgcgcatc tcgggcggcg cggtcttctg
     3301 cggttgttca tcgaccgcga aggcggcgtg acgctcgacg attgccagcg tgtcagcgag
     3361 cagatcggcg cgttcctcga cgtcgaggat ccgttgccgg gcagttacgt gctcgaggtg
     3421 tcgtcgccgg ggttcgatcg acggctgcga aagctcgagc actttatgcg cttcagcggc
     3481 gacaacgcga agatcgagct tcgggacgcg gtcgacggtc ggaggcgatt cacggggaag
     3541 ctcgtgggcg tcgagggcag cgaggtgctg ctcgaggttg acggtgagct aacgcgtttg
     3601 ccttttggag atattgcggt ggcgcgattg gcgccgtagc gcaaggtcgg tgacgagatg
     3661 agtaaagaaa ttttattggt agtcgacgct gtctcgaacg agaagggtgt cgagaagtcg
     3721 gtcattttcg aagccatcga ggcggcgctg gcgtccgcca cgaggaagcg ctacggcgac
     3781 gacatcgacg tgcgtgtctc gatcaaccgc acgaacggca gctacgagac gtttcgccgc
     3841 tggaaggtat tcgccgacga ttcgcgcgag atcgaagagc cggctcgcga gctgcggctc
     3901 gaggacgcgc tcgacatcga tccgaaagcg gaggtcggag gcttcgtcga acagccgatg
     3961 caatccgtcg agttcggccg catcgcggct cagaccg
//
LOCUS       DASSNN010000399         4768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_305026, whole genome shotgun sequence.
ACCESSION   DASSNN010000399 DASSNN010000000
VERSION     DASSNN010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305026"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VFL84_07420"
     CDS             complement(<1..1124)
                     /locus_tag="VFL84_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608488.1"
                     /translation="MATHAINDLAAHAFELVNKELSSTLDAARAELEGYVDGRSGPDA
                     LLKTAAHLHAARGALKIVEIHGAALLAEEMEQTCRKLAEAKDEQKSEQGVEALTRAMV
                     QLPAYLERLLGGGKDVALALLPLLNDLRQARDKPSLSEGTLVLLNAGPFERHLAQAPA
                     APVGPDVGRGFEKVAQRVRPGFQAALLGWIKGADAMRYVDELLRVSTTLERASVTEQV
                     KQLWTVMAAVLTAVRGGGLEASVALKRLIGQVDRQLKKLIDGGELALVNSPPVELVNS
                     LLYYVAKATSQDPRVEALRKQYSLGEVLPAQAQIEQARDGLAGPSVKLMRTVAHAIKE
                     DLGTVKDALDIFVRTGMQGADKLGPQLDMLKKIGDTLGVLG"
     gene            complement(1134..3242)
                     /locus_tag="VFL84_07425"
     CDS             complement(1134..3242)
                     /locus_tag="VFL84_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7608489.1"
                     /translation="MTKSSPWPKLLTPLGILLAVALLGAAGSVWWSGGGTTMVNDGGL
                     GELMAVTQAARAEANAAVLGEATAFEALAADRASMTSLRGAVAGNEAASADARALAGD
                     SALWQRIEKNLDTVLASREGLADLEKARAEIAELGPKLLQAAANVAGALPPADITANQ
                     PYLMRFEVTVESLQQRARALGPTVVVADAVRELDDAEQYIGQFVRGLRGEDATLGVVP
                     VKDGGAADLKSLSDLHERARQAVMAIGSEADTLTAVLAAIQDFDGAASELLTRYRDTD
                     ASIAAVVEGGLATRLPLLLVFAALAIAALMMFVYYRARDFREAAEQQAEQNERNQQAI
                     LRLLDELSSLADGDLTVQATVTEDITGAIADSINYAIEALRELVTTINDSSILVDAAS
                     KQTEGTARHLVRSAETQAKQAAAASESMARMAISIEEVSGNAERCSDVARHAVEISHK
                     GGEAVRRTIAGMNTIRETIQDTSKRIKRLGESSQEIGNIVELIEEIAEQTNILALNAS
                     IEASRAGEASRGFAVVADEVQKLAERSAGATKKIEVLVSTIQSDTNEAVMSMERSTTD
                     VVGGALLAENAGAALEEIEQVSHQIASLVQNISGSSKEQTSVAGAITKNMHVLREISA
                     KTTESTAAASTAISKLAELASQLRKSVSGFTLPDRGGGTGVLSAARVAASLERTEQVP
                     SLPEAAAAPPHNLERRRLSG"
     gene            complement(3239..3799)
                     /locus_tag="VFL84_07430"
     CDS             complement(3239..3799)
                     /locus_tag="VFL84_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7608490.1"
                     /translation="MNLQALAEQPFELLLELERRARAAIAAREGAPAAADEWVGIGFR
                     LGGERFVTSRADVREVLPIPEQVTRVPGAKPWLRGIANLRGQLLTVVDLKSFLGGAPA
                     SDRQARVLVVASREVPTGLIVDEVVGFRRFSSADYRDEVAPSVVRCEHYLEGSYSRGP
                     EAWPRFSLLKLLEDPLFLTAGETVRP"
     gene            complement(3796..4173)
                     /locus_tag="VFL84_07435"
     CDS             complement(3796..4173)
                     /locus_tag="VFL84_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7608491.1"
                     /translation="MALIMIVDDSPTEVHVLKTALEKHGFDTVSASDGSECLSLAREV
                     RPDLIFMDVVMPRVNGYQATRTLTRDPQTKSIPVVMITTKDQETDRIWGMRQGAVDYL
                     VKPVTEAVLVAKAQEVLERGSND"
     gene            complement(4186..4575)
                     /gene="pilG"
                     /locus_tag="VFL84_07440"
     CDS             complement(4186..4575)
                     /gene="pilG"
                     /locus_tag="VFL84_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HET7608492.1"
                     /translation="MNPKSDLQGLKILVIDDSKTIRRTAETLLAKEGCEVFTAVDGFD
                     ALSKIADHRPDIIFVDIMMPRLDGYQICSLIKHNKIFRDTPVIMLSSKDGLFDRARGR
                     LVGSEQYLTKPFTKDELLGAIQSHVAK"
BASE COUNT          723 a         1625 c         1575 g          845 t
ORIGIN      
        1 ccgagcacgc cgagcgtgtc gccgatcttc ttcagcatgt cgagctgcgg gcccagcttg
       61 tcggcaccct gcatgcccgt gcgcacgaag atgtcgagcg cgtctttgac ggtgccgagg
      121 tcctccttga tggcgtgggc caccgtgcgc atgagcttga cgctcgggcc ggcgagaccg
      181 tcgcgcgcct gctcgatctg cgcctgcgcc ggcaggacct cgcccaagct gtactgcttg
      241 cgcagcgcct cgacgcgcgg gtcctgcgac gtcgctttgg cgacgtaata aaggaggctg
      301 ttgacgagct cgacgggcgg cgagttcacg agcgcgagct ccccgccgtc gatgagcttc
      361 ttcagctgcc gatcgacctg gcctatgagg cgcttcagcg cgacgctggc ctcgagaccg
      421 ccgccgcgga cggccgtcag cacggccgcc atgacggtcc aaagctgctt gacttgctcc
      481 gtgaccgacg cgcgctcgag cgtcgtcgaa acgcgcaaca gctcgtcgac gtagcgcatg
      541 gcgtccgcgc ccttgatcca gccgagcagc gccgcctgga aaccggggcg aacacgctgc
      601 gcgaccttct cgaagccgcg gccgacgtcg gggcccacgg gagcggccgg cgcttgcgcg
      661 aggtggcgtt cgaacggacc ggcgttcagc aatacgagcg tgccctccga cagcgacggc
      721 ttgtcgcggg cctgacgcaa gtcgttcaag agcggcagca gcgccagcgc gacgtctttg
      781 ccgccgccga gcaaccgctc gagataagcc ggcagctgca ccatcgcgcg cgtcagcgct
      841 tcgacaccct gctcggattt ctgctcgtct ttcgcctcgg cgagctttcg gcaggtctgc
      901 tccatctcct cggcgagcaa cgccgcgccg tgaatctcga cgatcttcaa cgcgccgcgc
      961 gccgcatgca gatgcgcggc ggttttcagc aacgcgtccg ggcccgagcg gccgtcgacg
     1021 tagccttcga gctcggcgcg cgcggcatcg agcgtgctcg agagctcctt gttgacgagc
     1081 tcgaaagcgt gggcagccag gtcgttgatc gcgtgagtcg ccatggccct cgattaaccg
     1141 gacaggcgac ggcgttcgag gttgtgcggc ggcgcggcag ccgcctcggg caacgaaggc
     1201 acctgctccg tgcgctcgag gctcgccgcg acgcgcgcgg cgctcagcac gccggtgccg
     1261 ccgccgcggt cgggcagcgt aaatcccgaa acgctcttgc gcagctgcga cgcgagctcg
     1321 gcaagcttgc tgatcgcggt cgaggcggcc gcggtcgact cggtcgtttt cgcgctgatc
     1381 tcgcgcaaga cgtgcatgtt cttcgtgatc gcgcccgcga cgctcgtctg ctctttcgag
     1441 gagccggaaa tgttctggac gaggctcgcg atctgatgcg agacctgctc gatctcctcg
     1501 agcgcggcgc cggcgttctc ggcgagcaga gcgccgccga cgacgtccgt ggtgctgcgc
     1561 tccatggaca tcacggcctc gttcgtgtcg gactgaatcg tgctgacgag cacctcgatc
     1621 ttcttggtcg cgcccgccga gcgctcggcc agcttctgca cctcgtccgc gacgaccgcg
     1681 aatccgcggc tcgcctcgcc ggcgcgcgac gcctcgatcg aggcgttgag ggcgaggatg
     1741 ttcgtttgct cggcgatctc ctcgatgagc tcgacgatgt tgccgatctc ttgcgagctt
     1801 tcgcctaggc gtttgatgcg cttgctcgtg tcctgaatgg tctcgcggat cgtgttcatg
     1861 ccggcgatcg tgcgtcgcac ggcctcgccg cccttgtggg agatctcgac ggcgtggcgc
     1921 gcgacgtccg agcaccgctc ggcattgccc gagacctctt cgatcgagat cgccatccgc
     1981 gccatcgatt cggaagccgc cgcggcttgc ttcgcttgtg tctcggccga gcggacgaga
     2041 tgccgcgccg tgccctcggt ctgcttcgag gcggcgtcga cgagaatcga ggagtcgttg
     2101 atcgtcgtga cgagctcgcg cagcgcttcg atcgcatagt tgatcgaatc cgcgatcgcg
     2161 ccggtgatgt cctcggtcac cgttgcctgc acggtcagat cgccgtcggc caggctcgac
     2221 agctcgtcga gcaaccgcag gatcgcttgc tggttgcgct cgttctgctc ggcctgctgc
     2281 tcggctgcct cgcggaaatc tcgggcgcgg tagtagacga acatcatcaa cgccgcgatg
     2341 gccagcgccg cgaacacgag cagcagcggc aagcgcgtcg cgagcccgcc ctcgaccacg
     2401 gcggcgatcg acgcatcggt atcgcggtag cgcgtcagca gctcggacgc cgcgccgtcg
     2461 aagtcttgga tcgcggcgag cacggccgtc agcgtgtccg cctccgagcc gatggccatg
     2521 acggcctggc gcgcgcgctc gtgcaggtcc gacagcgatt tcaaatcggc tgcgccgccg
     2581 tctttgacgg ggacgactcc gagcgtcgcg tcctcgccgc gcaggccgcg cacaaactgg
     2641 ccgatgtatt gctcggcgtc gtcgagctcc cggacggcat cggcgacgac gacggtcggg
     2701 ccgagcgcgc gcgcgcgctg ctgcagcgac tcgaccgtga cctcgaagcg cattagataa
     2761 ggttggttcg ccgtgatatc ggcgggcggc agcgcgcccg cgacgttggc cgcggcctgg
     2821 agcagcttcg gcccgagctc ggcgatttca gcgcgcgcct tctcgaggtc ggcgagcccc
     2881 tcgcgcgacg cgagaacggt atcgagattc ttttcgatgc gctgccagag ggccgaatcg
     2941 cctgccaagg cgcgcgcgtc ggcgctggcc gcttcgttgc cggcgacggc gccgcgaagg
     3001 ctcgtcatcg aggcgcgatc ggcagcgagc gcctcgaagg ccgtcgcctc gccgagcacg
     3061 gccgcattcg cttccgcgcg agcggcttga gtcacggcca tgagctcgcc gagcccgccg
     3121 tcgttaacca ttgtcgtgcc gccgccgctc caccaaaccg atccggccgc gccgagcagg
     3181 gccacggcga gcaggattcc gagcggcgtc agtagtttcg gccacgggct cgatttcgtc
     3241 atggcctcac cgtctccccc gccgtcagaa acagcgggtc ctcgagcaat ttgagcaggc
     3301 tgaatcgcgg ccacgcctcg ggaccgcggc tatagctgcc ttctaggtag tgctcgcagc
     3361 gaacgacgct cggggccacc tcgtcgcgat agtcggccga gctgaagcgt cggaacccga
     3421 ccacctcgtc gacgatgagg ccggtcggca cctcgcggct cgcgacgacg agcacccgag
     3481 cctgccgatc cgaggcgggc gcgccgccta ggaatgactt cagatcgacg accgtcagga
     3541 gttggccgcg caagttcgcg atgccgcgaa gccacggctt ggcgccgggc actctagtga
     3601 cttgctcggg gatcggcagc acctcgcgga cgtcggcgcg gctcgtcacg aaccgctcgc
     3661 cgccgaggcg aaagccgatg ccgacccatt cgtccgccgc ggccggcgca ccctcgcgcg
     3721 cggcgatggc ggcgcgggca cggcgctcga gctcgaggag cagctcgaac ggttgctcgg
     3781 cgagcgcttg caggttcaat cgttcgaccc ccgctcgagc acttcctgcg ccttggcgac
     3841 gagcacggct tccgtgacgg gcttgacgag gtagtcgacc gcgccctggc gcattcccca
     3901 gatgcggtcg gtttcctggt ccttggtcgt gatcatgacg accgggatcg acttcgtttg
     3961 cggatcgcga gtcagcgtgc gagtggcttg atagccgttg acgcgcggca tcacgacgtc
     4021 catgaaaatc aggtccgggc gcacctcgcg ggcgaggctc aagcactcgg agccgtccga
     4081 cgccgaaacg gtgtcgaagc cgtgcttctc gagggccgtc ttcaagacgt gcacctcggt
     4141 gggcgagtcg tcgacgatca tgatcaaggc catagacgct tccgcttatt tggccacgtg
     4201 gctctgaatc gcgccgagca gctcatcttt cgtgaacggt ttggtcagat actgctccga
     4261 gccgacgagc cggccccgag ctcgatcgaa caacccgtcc ttgctcgaca acatgatgac
     4321 gggcgtgtcc ctgaagatct tgttgtgctt gatcaacgag caaatctgat agccgtcgag
     4381 gcgcggcatc atgatgtcga cgaaaatgat gtccggccgg tggtccgcaa tcttcgaaag
     4441 cgcgtcgaac ccgtcgacgg cagtgaacac ctcgcaccct tctttcgcga gtaacgtctc
     4501 ggcggttctg cggatcgttt tgctgtcgtc gatgacgaga atcttgagcc cttgcaagtc
     4561 gcttttcggg ttcactgcac tgctgccaac cacttatatt tcctccccga agccgcgcgc
     4621 cgcttgacgg ctcggagccg agcacgaccc ttaggctcac cgcgcctttt aacatatcgg
     4681 ggccagccct tccatcgact ggcacgcatg cattcatgac gttcggcggg cgcaagagcg
     4741 cgctccccaa tcgacgaacg ttcgacag
//
LOCUS       DASSNN010000400         3787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1818798, whole genome shotgun sequence.
ACCESSION   DASSNN010000400 DASSNN010000000
VERSION     DASSNN010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818798"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1070
                     /gene="hemE"
                     /locus_tag="VFL84_07445"
     CDS             45..1070
                     /gene="hemE"
                     /locus_tag="VFL84_07445"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET7608493.1"
                     /translation="MPQSDDKLLLRAIRREAVPRRPLWIMRQAGRYLPEYREFRSKHS
                     FKELAGKAELAAEVTLMPLKRFPFDAAIVFADIMSPMPSLGIEFDFDPGPVVANPIRD
                     GAAIERLRRPDAQEIAPEVIEALKLARAALPKSTALLGFCGAPWTLAAYLVEGKGAKD
                     FPTLRAFAAAEPQLLGALLERLTSLMGEYLKQQAAAGADAVQIFDSWAGLLSRASWEK
                     LIRPHLTRLLEAVGQVGIPRILFLQNAPHLMEAYASLPAEVLGVDWRVDLPELKRRHP
                     DRAVQGNLEPAILTAGPDATAAAAKALLAETPARGHIVNLGHGILPQTPLASVAALVD
                     AVHSEEL"
     gene            1067..1807
                     /locus_tag="VFL84_07450"
     CDS             1067..1807
                     /locus_tag="VFL84_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HET7608494.1"
                     /translation="MSDRPAATDAADDTQPLDLAEKGRRGGQAISLDRRLFMKFTAFG
                     GCADADAAIAALTEDGVTGALYLDASDPQGIGLIATAEDPEYFVTTLRSLLNREPFAS
                     LTHKPELDMLGRTYSIGYEPDLEETLFKRPLSKITDLKNRWAIWYPLQRSKKFQTLPA
                     DHQRRILAEHGSLAKRYGAGGHANDVRLACHGLDKNDNDFIVGLLGPNLHPLSAVVQE
                     MRKTEQTSQYLDSLGPFFVGRAAWQAKP"
     gene            1789..2883
                     /gene="hemH"
                     /locus_tag="VFL84_07455"
     CDS             1789..2883
                     /gene="hemH"
                     /locus_tag="VFL84_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET7608495.1"
                     /translation="MASKALNKGHKSHDYDALLVVSFGGPEGPADVDSFLDNVFRGLR
                     VPPETRAHIAKRYEHVGGVSPINAHTRAFIKALQLELDAHGPALPIYWGNRNWHPLLK
                     DTVAQMAHAGVRRALAFVTSTFSSYSGCRRYREDLYEAASGVPNAPRFDKLRVPYNHP
                     GFIEAMSDRVNEAIETLPAAKREHALVLFTAHSLPVAMARGCAYETQLAESCKLVGDA
                     LKLHRWRLVYQSNNASYGREPWLGPDIRDALREAEAEGIKAVVVAPIGFACDHMEVIM
                     DLDVDAAAVAREIGLTMARAGTVGTHPKYVAMVRELIVERLTPDAPRRALGSLGPSHD
                     RCAADCCLPGRPGPLKPALCGVDDPSLEPD"
     gene            2888..>3787
                     /locus_tag="VFL84_07460"
     CDS             2888..>3787
                     /locus_tag="VFL84_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7608496.1"
                     /translation="MAATDFDVIVVGAGLAGLHAAATLESLGMRVLVLEAQRRVGGRV
                     HSMRQLGGTAEAGGTYIGAGYERVIAAAERHGVELMDVTPVLEFFREQDLALDGEIIR
                     QDEWATHPKNDFPQRDKEHLPWTYHRVLTMRDNPLEDPSEWLDARHAALDVSAHDWFR
                     SLGLSERAIALAYGLNVSFGRDARDVSALLLLFRGAFSKQQRARSSSSSLGFTARHGV
                     QLIPDAMAAALGIGVELDHAVTAIALESDRAAVVCANGRRLGARYVIAAVPPSVLRRI
                     AIEPKLPPLQAEAIATLPAQPLTQ"
BASE COUNT          609 a         1314 c         1283 g          581 t
ORIGIN      
        1 gtcgagccgc agcagagcgg cgcgggcgag tgacacgagc tccaatgccg caatccgacg
       61 acaagctgct gctcagagcc attcggcgcg aagccgtgcc gcgccggccg ctgtggatca
      121 tgcgccaagc gggccgctac ctgcccgagt atcgcgagtt ccgctcgaag cacagcttca
      181 aggagctcgc cggcaaggcc gagctcgcgg ccgaagtgac gctgatgccg ctgaaacgct
      241 tcccgttcga cgcggcgatt gttttcgccg acatcatgag cccgatgccg tcgctcggaa
      301 tcgagttcga tttcgatccc ggccccgtcg tcgcgaaccc gatccgtgac ggagccgcga
      361 tcgagcggtt gcgccgcccg gacgcgcagg agatcgcgcc cgaggtcatc gaagcactga
      421 agctcgcgcg cgccgcgctg ccgaagagca ccgcgctgct cggcttctgc ggcgcgccgt
      481 ggacgctcgc ggcctatctc gtcgaaggca agggcgcgaa ggattttccg acgctgcgcg
      541 cattcgcggc cgcggagccg cagctgctcg gcgcgctgct cgagcgcctc acgagcctga
      601 tgggcgagta cctgaagcag caggccgcgg ccggcgccga cgccgtgcag atcttcgact
      661 cgtgggccgg gttgctgtcg cgcgcgagct gggagaagct gattcgaccg catttgacgc
      721 ggttgctcga ggcggtcggc caggttggta ttccacgtat cctgttcctg cagaacgcgc
      781 cgcacctcat ggaggcctac gcgagcctgc cggccgaagt gctcggcgtc gactggagag
      841 tcgatttgcc ggagctcaag cggcgccatc cggaccgggc cgtgcaaggc aatctcgagc
      901 ctgcgattct tacggcgggg ccggacgcga cggcggcggc agccaaagct ctgctcgccg
      961 agacgccggc gcgcggccat atcgtaaatt tggggcatgg catcctgccg cagacgccgc
     1021 ttgcgagcgt cgcggcgctc gtcgacgccg tgcactcgga ggaattatga gcgacagacc
     1081 cgccgcgacc gacgcggccg acgacacgca accgctggac ctcgccgaga agggccgccg
     1141 cggcggacag gcgatttcgc tcgaccggcg gctgttcatg aagttcacgg cgttcggcgg
     1201 ctgcgcggac gccgacgcgg cgatcgcggc gctcacggaa gacggcgtca cgggcgcgct
     1261 ctatctcgac gcgagcgacc cgcaaggcat cggcctcatc gcgacggccg aggaccccga
     1321 gtacttcgtg acgacgctgc gctcgcttct gaaccgcgag ccgttcgcgt cattgaccca
     1381 taagcccgag ctcgacatgc tcggccgaac ctattcgatc ggctacgagc cggatctcga
     1441 ggagacgctg ttcaagcggc cgctgtcgaa gatcacggac cttaagaatc gctgggccat
     1501 ctggtatccg ctccagcgct cgaaaaaatt ccagacgctg ccggccgatc accagcgccg
     1561 cattctcgcc gagcacggct cgctcgcgaa acgctacggc gcgggcggcc acgccaacga
     1621 cgtgcggctc gcctgccacg gcctcgacaa gaacgacaac gacttcatcg tcgggctctt
     1681 gggcccgaac ctgcatccgt tgtcggccgt cgtgcaggag atgcgcaaga ccgagcagac
     1741 gtcgcaatac ctcgacagct tgggcccgtt cttcgtcggg cgtgccgcat ggcaagcaaa
     1801 gccctgaaca aaggacacaa gtcgcacgac tacgacgcgc tgctcgtcgt gagtttcggc
     1861 gggccggaag gtcccgccga cgtcgactcg ttcctcgaca acgtcttccg gggcctgcgc
     1921 gtgccgccgg agacgcgagc gcacatcgcg aagcgctacg agcacgtcgg cggcgtgagc
     1981 ccgatcaatg cgcacacgcg cgctttcatc aaggcgctgc agctcgagct cgacgcgcac
     2041 ggccccgcgc tgccgatcta ctggggcaat cgcaattggc acccgctgct caaggacacg
     2101 gtcgcccaga tggcgcacgc cggcgtgcgc cgcgcgctcg cgttcgtgac gagcacgttc
     2161 agctcctata gcggttgccg ccgctatcgc gaggacttgt acgaggccgc gagcggcgtg
     2221 ccaaacgctc cgcgcttcga caagctgcgc gtgccgtaca accatcccgg ctttatcgag
     2281 gcgatgagcg accgcgtcaa cgaagcgatc gagacgctgc ccgcggcgaa gcgcgagcac
     2341 gctctcgtgc tgttcactgc gcacagccta cccgtggcga tggcgcgcgg ctgcgcctac
     2401 gagacgcaac tggcggagag ctgcaagctc gtcggcgacg cgctcaagct tcaccgttgg
     2461 cggctcgtct atcagagcaa caacgcgtcc tatggccgcg agccgtggct cggccccgac
     2521 atccgcgacg cgctgcgcga agcggaagcc gaaggcatca aggccgtcgt cgtcgcgccg
     2581 atcggcttcg cttgcgatca catggaagtc atcatggatc tcgacgtcga cgcggccgcc
     2641 gtcgcgcgcg agatcggtct caccatggcg cgcgcgggca cggtcggtac gcatcccaag
     2701 tacgtcgcga tggttcgcga gctcatcgtc gagcgtctga cgcccgacgc accgcggcgc
     2761 gcgctcggct cgctcggccc gagtcacgac cgctgcgcgg ccgactgctg cctgcccggc
     2821 cgtccgggac cgctgaagcc ggcgctttgc ggcgtcgacg atccgtcgct cgagccggat
     2881 tgaggcgttg gccgctaccg atttcgacgt catcgtcgtc ggcgccgggc tcgcgggcct
     2941 gcatgccgcc gcgacgctcg agagcttagg catgcgcgtg ctcgttctcg aggcgcagcg
     3001 gcgcgtcggc ggccgcgtgc attcgatgcg tcagctgggc gggacggcgg aggccggcgg
     3061 cacgtacatc ggcgccggct acgagcgcgt catcgcggcc gcggagcgcc acggcgtcga
     3121 gctcatggac gtcacgccgg tcctcgagtt cttccgcgag caagacctgg cgctcgacgg
     3181 cgagatcatc cggcaagacg agtgggcgac gcatccgaag aacgatttcc cgcagcgcga
     3241 caaagagcat ctgccgtgga cctatcaccg cgtgctcacg atgcgcgaca accctttaga
     3301 ggacccgagc gagtggctcg acgcgcgcca tgccgcgctc gacgtttccg cgcacgattg
     3361 gttccgcagc ctgggtttga gcgagcgtgc gattgcgctc gcgtacgggc tcaacgtgag
     3421 cttcggccgc gatgcgcgcg acgtctcggc gctgctgctg ctctttcgcg gcgcgttctc
     3481 gaagcagcaa cgcgcgcgga gctcatcgtc gagcctcggc ttcacggcgc gccacggcgt
     3541 gcagctgata ccggacgcga tggcggcggc gcttggcata ggcgtcgagc tcgaccacgc
     3601 cgtgacggcg atcgcgctcg agagcgatcg cgccgccgtc gtttgcgcga acggccggcg
     3661 gctcggcgca cggtacgtca tcgcggccgt gccgccgtcc gtgctgcggc gcatcgcgat
     3721 cgagcccaag cttccgccgc ttcaagccga ggcgatcgcg acgttgccgg cgcagccgct
     3781 gacgcag
//
LOCUS       DASSNN010000401         4121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_933726, whole genome shotgun sequence.
ACCESSION   DASSNN010000401 DASSNN010000000
VERSION     DASSNN010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933726"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..2654
                     /gene="nirB"
                     /locus_tag="VFL84_07465"
     CDS             189..2654
                     /gene="nirB"
                     /locus_tag="VFL84_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HET7608497.1"
                     /translation="MKRKLVLVGNGMAGMRAIEELLKLEPHMYDITVFGSEPHGNYNR
                     ILLSPVLAAEKTVADIMLNTREWYADNDIALHAGDEVVRIDRARRRVVARSGLEAPYD
                     RLILATGSNPVVLPLPGKDLPGVVSYRDIADVDRMIAASRTFPRAVVIGGGLLGLEAA
                     YGLQKRGMDVTVVHLVATLMERQLDASAATLLKQSLEQRGLKFVMPAQTAAIVGDERA
                     RAVKLADGTEVPADLVVMAVGIRPNVALAKASGIACERGVLVDDTMQTYDPRIYALGE
                     CVQHRGSCYGLVAPLFEQAKVVANHLAELGIGRYAGSQTSTKLKVTGIDLFSAGDFAG
                     GGSTEELVFKDAARGVYKKLVVEGNKLKGAVLYGDTVDGAWYFQLMRDGIEIGDFREN
                     ILFGQAHIGDSGTAGADAVTALADTAEICGCNGVCKGDIVKAITKKNLFTLDEVRAHT
                     KASSSCGSCTGLVEQLLANTLGGDYSEAPAKKPMCPCTIHTHEEVRAAIVREKLKSIP
                     AAMLFLEWKTPDGCHKCRPGLNFYLLCAWPGEYRDDKQSRFINERAHGNIQRDGTYSV
                     VPRIWGGVTSVKELRAIADVAEKYAVPTVKVTGGQRIDLLGVKKEDLPGVWRDLSAAG
                     FVSGHAYGKALRTVKTCVGKQWCRFGTQDSTGLGIKLERVTWGSWHPHKVKLAVSGCP
                     RNCAEATIKDLGVVCVDSGYELHVGGNGGIKIRATDFLCKVATQGEVVEYCCAYLQLY
                     REEARYLERTAPWIERVGLSHVRERIVDDAQARKALYARFLESQKHSQIDPWAERAAG
                     VEAEEFAPLRAEPRPEPLAELAP"
     gene            2651..2983
                     /gene="nirD"
                     /locus_tag="VFL84_07470"
     CDS             2651..2983
                     /gene="nirD"
                     /locus_tag="VFL84_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HET7608498.1"
                     /translation="MSWVDVGPVAALPERGARCVRIGGLSVAVFRTSTGRVFALRDQC
                     PHRGGPLSQGIVHGTRVTCPLHEWVIDLKTGQATGIDEGATATFNVRVVDGRIALELP
                     AEALAKAS"
     gene            2980..>4121
                     /locus_tag="VFL84_07475"
     CDS             2980..>4121
                     /locus_tag="VFL84_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7608499.1"
                     /translation="MTAVRTTCPYCGVGCGLRAERAADGVYAIRGDPEHPSNGGRVCS
                     KGAALGETLGLEGRVLHPLIRGARVTWDQALAAVADGFARTIAAHGPDSVAFYVSGQL
                     LTEDYYVANKLMKGFIGSGNIDTNSRLCMASAVAGYKRAFGADAVPNDYTDLERAELV
                     VLVGSNLAWCHPVLFQRLSAAKAANPKLKVVLVDPRRTQTADVADLHLQLAPGTDVVL
                     FNGLLNHLRREDALDLEFLDAHTEGFGAALRCAKETAGSIPAVAVACGLAEDDVLELY
                     RWFARTPLTVTAFSQGVNQSTSGTDKVNAIVNVHLATGRIGKPGMGPFSLTGQPNAMG
                     GREVGALANQLAAHMDFSAENVERVARFWRTARVAAKPGLKAVDLF"
BASE COUNT          683 a         1359 c         1449 g          630 t
ORIGIN      
        1 cgccgaggtg ggcggcgcac acttcggcga ggagctcgat ctgcagctgg tcgcgcgcac
       61 ggagcgcatg gcgttaaccg ccaagtacgc ggattaccga gccgaccagc tcttgacgga
      121 taccgagaag gtctggctct cggtcgacta cgcgttctga tcaagagagc cggcgcgaag
      181 gagggaggat gaagcgaaag ctcgtgctcg tagggaacgg gatggccggc atgcgcgcca
      241 tcgaggagct cttgaagctc gagccgcaca tgtacgacat cacggtgttc ggcagcgagc
      301 cgcacggcaa ctacaaccgc atcctgttat cgcccgtgct ggcggcggag aagaccgtcg
      361 ccgacatcat gttgaacacg cgcgagtggt acgccgacaa cgacatcgcg ctgcacgcgg
      421 gcgacgaggt cgtgcgcatc gatcgcgcgc gtcgtcgcgt cgtcgcgagg agcggcctcg
      481 aggcgcccta cgatcggctg attctcgcga cgggctcgaa cccggtcgtg ctgccgctgc
      541 ccggcaagga cctgcccggc gtcgtcagct accgcgacat cgcggacgtc gatcgcatga
      601 tcgcggcgtc ccggacgttc ccgcgcgccg tcgtcatcgg cggcgggctg ctcggcctcg
      661 aggcggctta cgggctgcag aagcgcggca tggatgtcac ggtcgtgcac ctcgtcgcga
      721 cgctgatgga gcgccagctc gacgcgtccg ccgcgacgct gttgaagcag tcgctcgagc
      781 aacgcggtct caagttcgtg atgccggcgc agacggcggc gatcgtcggc gacgagcgtg
      841 cgcgcgccgt caagctcgct gacggcaccg aggtgccggc cgacctcgtc gtcatggccg
      901 tcggcattcg gccgaacgtc gcgctcgcaa aggcgagcgg catcgcctgc gagcgcggcg
      961 tgctcgtcga cgacacgatg cagacttacg acccgcgcat ctacgcgctc ggcgagtgcg
     1021 tgcagcatcg cggctcttgc tacggcctcg tcgcgccgct gttcgagcag gcgaaggtcg
     1081 tcgcgaatca tctcgccgag ctcggcatcg gccgctatgc gggctcgcag acctcgacga
     1141 agctcaaggt cacgggcatc gacctctttt cggccggtga ttttgcgggc ggcggctcta
     1201 ccgaggagct cgtgttcaag gacgcggcgc gcggcgtgta caagaagctc gtcgtcgagg
     1261 gcaacaagct caagggcgcc gtgctgtacg gcgataccgt cgacggcgcg tggtacttcc
     1321 agctcatgcg cgacggcatc gagattggcg atttccgcga gaacatcctg ttcggccagg
     1381 cgcacatcgg cgactccggc acggccggcg cagacgccgt cactgcgctc gccgataccg
     1441 cggagatctg cggctgcaac ggcgtctgca aaggcgacat cgtcaaggcg atcacgaaga
     1501 agaatctgtt cacgctcgac gaggtgcgcg cgcatacgaa ggcttcgtcc tcgtgcggct
     1561 cgtgcacggg cctggtcgag caactgctcg caaatacgtt gggcggcgac tattcggaag
     1621 cgccggcgaa gaagccgatg tgcccatgca cgatccacac gcacgaagag gtgcgagccg
     1681 cgatcgtgcg cgagaagctg aagtcgattc ccgccgcgat gctgttcctc gaatggaaga
     1741 cgcccgacgg ctgccacaag tgccggcccg ggctcaactt ctacctgctg tgcgcgtggc
     1801 ccggcgaata tcgcgacgac aagcagtcgc ggttcatcaa cgagcgcgcg cacggcaaca
     1861 tccagcgcga cggcacgtat tccgtcgtgc cgcgaatctg gggcggcgtg acgtcggtga
     1921 aggagctgcg cgcgatcgcg gacgtcgcag agaagtacgc cgtgccgacc gtcaaggtca
     1981 cgggcggcca gcgcatcgac ctgctcggcg tcaagaaaga ggacctgccg ggcgtctggc
     2041 gcgacctgag cgcggccgga ttcgtctcgg gtcacgccta cggcaaagcg ctgcgaaccg
     2101 tgaagacctg cgtcgggaaa cagtggtgcc ggttcggcac gcaggactcg acagggctcg
     2161 gcatcaagct cgagcgcgtc acgtggggct cctggcaccc gcacaaggtc aagctggccg
     2221 tctcgggctg cccgcgcaac tgtgccgagg cgacgatcaa ggacttaggt gtcgtctgcg
     2281 tcgactcggg ctacgagctg cacgtcggcg gcaacggcgg aatcaagatc cgcgcgacgg
     2341 acttcctctg caaagtcgca acgcagggcg aggtcgtcga gtactgctgc gcgtacttgc
     2401 agctctaccg cgaggaagcg cgctatctcg agcgcacggc gccgtggatc gagcgcgtcg
     2461 ggctctcgca cgtgcgcgag cgcatcgtcg acgacgcgca ggcgcgcaag gcgctctacg
     2521 cacggttcct cgaatcgcag aagcactcgc agatcgatcc gtgggccgag cgcgcggcag
     2581 gcgtcgaggc cgaggagttc gcgccgctgc gcgcggagcc gcggcccgag ccgctcgcgg
     2641 agctcgcgcc gtgagctggg tcgacgtggg gccggtcgcg gcgctgccgg agcgcggcgc
     2701 gcgctgcgtc cgcatcggcg ggctcagcgt agccgtattc cggacgagca cgggccgggt
     2761 gttcgcgctg cgcgatcagt gcccgcatcg cggcgggccg ctgtcgcaag gcatcgtgca
     2821 cggcacgcgc gtgacctgcc cgctgcacga gtgggtcatc gatcttaaga cggggcaggc
     2881 gaccggcatc gacgaaggcg cgacggcgac gttcaacgtt cgcgtcgtcg acgggcgaat
     2941 cgcattggag ctgcccgcgg aagcgctcgc aaaggcttca tgacggccgt gcgcacgact
     3001 tgcccgtact gcggcgtcgg ctgcggcctg cgggccgagc gcgcggcgga cggcgtctac
     3061 gcgattcgcg gcgatcccga gcatccgtcg aacggcgggc gcgtctgctc gaaaggcgcc
     3121 gcgctcggcg agacgctcgg gctcgaaggg cgggtgcttc atcctttgat tcgcggcgcg
     3181 cgagtgacct gggaccaagc gctcgccgcc gtcgccgacg ggttcgcgcg cacgatcgcc
     3241 gcgcacgggc ccgactcggt cgcgttctat gtgtcgggcc agctcctgac cgaggactat
     3301 tacgtcgcaa acaagctcat gaagggcttc atcggcagcg gcaacatcga cacgaactcg
     3361 cggctctgca tggcgtcggc cgtcgccggc tacaagcgcg cgttcggcgc cgacgcggtg
     3421 ccgaacgact acacggatct cgagcgagcg gagctcgtcg tgctcgtcgg ctcgaatctc
     3481 gcctggtgcc atcccgtgct gttccaacgc ttgagcgcgg cgaaggccgc caatcctaag
     3541 ctcaaggtcg tgctcgtcga tccgcgccgc acgcaaacgg cggacgtcgc ggacttgcac
     3601 ttgcagctcg cgcccggcac cgacgtcgtg ctgttcaacg gcctcttgaa tcacctgcgc
     3661 cgcgaggacg cgctcgatct cgagttcctc gacgctcaca cggaggggtt cggcgccgcg
     3721 ctgcgttgcg cgaaggagac cgcggggtcg atccccgccg tggccgtagc gtgcgggctc
     3781 gccgaggacg atgtgctcga gctgtaccga tggttcgcgc gcacgccgtt gaccgtcaca
     3841 gcgttctcgc aaggcgtcaa ccaatcgacg agcggcaccg acaaggtcaa cgcgatcgtg
     3901 aacgtgcacc tcgcgacggg acgcatcggc aagccgggca tggggccgtt ctcgttgacg
     3961 ggccaaccga acgcgatggg cggacgcgag gtcggcgcgc tcgcgaacca gctcgcggcc
     4021 cacatggact tctccgcgga aaacgtcgag cgagtcgcgc gcttctggcg cacggcgcgc
     4081 gtcgcggcca aaccggggct caaggccgtc gatctgttcg a
//
LOCUS       DASSNN010000402         3478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1819278, whole genome shotgun sequence.
ACCESSION   DASSNN010000402 DASSNN010000000
VERSION     DASSNN010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819278"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VFL84_07480"
     CDS             <1..765
                     /locus_tag="VFL84_07480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7608500.1"
                     /translation="NVSVLSPFSWLRYLNPRVDRIVCVAEAIRRYFLGLRLLWWRLPP
                     EKVVTIYKGHDLAWYRDAPVDLASVGVPADAFSVCCVANWRPRKGVEVLIRAFALLPS
                     EARVHLVLAGDMQSPELARLIAAQPHRERIHVLGMRTDAPALAGACNVAVLPSLRREG
                     LPKTVIEAMAYGVPTIVTDVGGSAELVTDGVNGLVVAPGDSEALAAAIQRLQRDPALA
                     RELGRRGRERIEREFSNRATIARTAALYRELVEAPR"
     gene            762..1475
                     /locus_tag="VFL84_07485"
     CDS             762..1475
                     /locus_tag="VFL84_07485"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET7608501.1"
                     /translation="MSGFKESVRALLRKLRRGSTATRNARQLLRGFDAFGIDLVFDVG
                     ANTGQFAREIRDNGFRGRIVSFEPLSHAHRQLTAAAASDPSWSVHERCALGDKAGSAV
                     LNIAGNSVSSSVLEMAAAHEAAAPGSAYVGREETALVTLDSVADRYLGDSERPFLKID
                     TQGFEWQVLTGAEKTLPSIRGVLCELSLVTLYEGQHLWREVVDRLEESGFTLWGVQPS
                     FMDQRGRNLQADAIFFRER"
     gene            complement(1514..1819)
                     /locus_tag="VFL84_07490"
     CDS             complement(1514..1819)
                     /locus_tag="VFL84_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608502.1"
                     /translation="MPKKERDGQLPLELTFTDDHLGAASFAPSPGSDGGLFSCSIGPL
                     TEETVGDLLRAARTQTPIRLRFSERPLLLSHVTVERKGPHSVRLVGHVVKGGTRETA"
     gene            complement(2080..2571)
                     /locus_tag="VFL84_07495"
     CDS             complement(2080..2571)
                     /locus_tag="VFL84_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7608503.1"
                     /translation="MNRTTVERKSDREVVVTRTFDAPARLVFEAWTKPDLFKQWWVPR
                     SMGMTLRSCELDVRTGGKYRLVFGDDVANPMAFFGTYLEVVPNQRIVLTNEESGADGS
                     VTTVTFDEKNGKTTVVLRELYPTKEALDGAGTGAQEVMHETFEQLDELLASLATKTIH
                     ESH"
     gene            complement(2568..2945)
                     /locus_tag="VFL84_07500"
     CDS             complement(2568..2945)
                     /locus_tag="VFL84_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7608504.1"
                     /translation="MVQYSDTHFNASFAALADATRRGVLEQLARGDASITDLAGKFDM
                     TLTGMKKHVAVLEQAGLVATEKIGRVRTCKIGPCRLEEEMEWISRYRQVWDARFEALD
                     RVIERLKSRDKPDVRKKRKARPA"
     gene            3107..3466
                     /locus_tag="VFL84_07505"
     CDS             3107..3466
                     /locus_tag="VFL84_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7608505.1"
                     /translation="MRATVMIVVSVLAIVSTAKAQTSTATVPSADGTPIAFECAGAGP
                     SLVIVHGGTGDRTRWTPLFPLLAAHFRVCAMDRRAHGASGDAPSYSLAKEVEDVVAVV
                     EALSSAITPQRWREWTN"
BASE COUNT          563 a         1084 c         1192 g          639 t
ORIGIN      
        1 aacgtgagcg tgttgagccc gttctcgtgg ctgcgctact tgaatccgcg cgtcgaccgg
       61 atcgtttgcg tcgccgaagc gattcgccgg tatttcctgg gcttgcgctt gctctggtgg
      121 cggctgccgc ctgagaaggt cgtaacgatc tacaagggcc acgacctcgc ctggtaccgc
      181 gacgcgcccg tcgacctcgc gtccgtcggc gtcccggcgg atgcgttcag cgtctgctgc
      241 gtcgcgaact ggcggccgcg caagggagtc gaggtcttga tccgcgcgtt cgcgttgttg
      301 ccgagtgagg cgcgcgtcca cctcgtcttg gccggcgaca tgcagagccc ggagctcgcg
      361 cggctcatcg cggcgcaacc gcatcgcgag cgcatccacg tgctcggcat gcggaccgac
      421 gcgccggcgc tggcgggcgc ctgcaacgtc gccgtccttc cgtcgctgcg gcgcgaaggt
      481 ttgcccaaga ccgtgatcga ggcaatggcg tacggcgttc caaccatcgt gacggatgtc
      541 ggcggcagcg ccgagctcgt gacggacggc gtgaacggcc tcgtcgtcgc gcctggcgac
      601 agcgaggcgc tggccgcggc catacagcgg ttgcagcgag atcccgcgct cgcgcgtgag
      661 ctcgggcggc gcggccgtga gcgaatcgag cgcgaattct cgaaccgagc cacgatcgcg
      721 cgcacggccg cgttgtatcg cgagctcgtc gaggcgccgc gatgagcggt ttcaaggaga
      781 gcgttcgcgc gctgctgagg aagcttcgcc gcggctcgac ggcaacgcgc aacgcacggc
      841 agctgctccg cggcttcgac gcgttcggca tcgatctggt cttcgacgtc ggcgcaaaca
      901 ccggccagtt tgcgcgcgag atccgcgaca acgggttccg gggacgtatc gtgagcttcg
      961 agccgctgtc tcacgcgcat cgacagctca cggcggccgc ggcaagcgat ccgtcgtggt
     1021 ccgtgcacga acgttgcgcg ctgggcgaca aagcaggttc cgccgtcctc aacatcgcgg
     1081 gcaattcggt gtcatcgtcc gttctcgaga tggccgccgc tcacgaggcc gctgcgccgg
     1141 gatcggccta tgtcggacgc gaggaaaccg cgctggtgac gttggactcc gtcgctgacc
     1201 ggtatctcgg agattcagag cgcccgttcc tcaagatcga tacgcaggga ttcgaatggc
     1261 aggtgctgac gggggcggag aaaacgttgc cgtcgatccg aggcgttctg tgcgagctgt
     1321 ctctcgtcac gctgtacgag ggccaacacc tttggcggga ggtggtggac cgcctcgaag
     1381 aatcgggctt cacgctatgg ggcgtgcagc caagcttcat ggaccaacgc gggcgcaacc
     1441 tccaggccga cgcgattttt ttccgcgagc gttgagggcg accaagcctg cgtcggagag
     1501 cggcgagcgc tcgctacgcc gtttccctcg tgccgccttt gacgacatgt ccgacgaggc
     1561 gaacggaatg cggccccttg cgctcgacgg tcacatgact gagcagcaac ggacgctcgc
     1621 tgaatcgcaa gcgaatcggc gtttgagtgc gcgctgcgcg cagcagatcg cccaccgttt
     1681 cttcggtgag gggcccgatc gagcacgaga acaaccctcc gtcactgccc ggcgacggcg
     1741 cgaacgacgc ggcaccgagg tggtcgtcgg tgaaagtaag ctcgaggggc aactgaccgt
     1801 cgcgttcttt ttttggcatt ccgggagaga agctggttgc cgagacgcgc gggctcagtg
     1861 tacacggtcc ccggcatgcg acctaatcta tttcgaatcg cggaaccaaa cggacggtcc
     1921 tgtcgtccgt gtagtggtca tgcgggtaag agctttgcgt gtcgtgggca gcgcgtcgga
     1981 ctggccaacg cttttgaagg actatcaagc ggctgtgaaa aacttcgaga gcgtttcgaa
     2041 cgtgcacgac gcgagctagg cttgaggctc acgcccggct caatgtgact cgtggatagt
     2101 ttttgtggcg agcgaggcga gcagctcgtc gagctgctcg aacgtctcgt gcatgacttc
     2161 ctgcgcgccg gtgccggcgc cgtcgagcgc ttccttcgtc gggtagagct cgcggagcac
     2221 caccgtcgtc ttgccgtttt tctcatcgaa ggtgacggtg gtgacggaac cgtcggcgcc
     2281 gctttcctca ttcgtcaaga cgatgcgctg gttcggaacc acctcgaggt acgtcccgaa
     2341 gaaagccatc gggttcgcaa cgtcgtcgcc gaacacgagg cggtatttac cgccggtgcg
     2401 cacgtccagc tcgcacgaac gcagcgtcat gcccatcgac cgcggcaccc accattgctt
     2461 gaacagatcg ggcttagtcc acgcctcgaa cacgaggcgc gcgggcgcgt cgaacgtgcg
     2521 ggtgacgacg acttcgcgat cggacttgcg ttcaacggtc gtgcgattca tgcgggcctc
     2581 gcttttctct tcttgcggac atcgggtttg tcgcgggact tgagccgctc gatcacgcgg
     2641 tccagcgctt cgaagcgggc atcccagact tggcggtagc gcgagatcca ctccatttcc
     2701 tcttcgagcc ggcaggggcc gatcttgcag gtgcgcacgc ggccgatctt ttcggtggcc
     2761 acgagcccgg cctgctcgag cacggcgacg tgtttcttca tgccggtgag ggtcatgtcg
     2821 aacttgccgg caaggtcggt gatcgaggcg tcgccgcgcg cgagctgctc gagcacgccg
     2881 cgacgggtgg cgtccgcgag cgcggcgaag gaggcgttga agtgggtgtc tgaatactga
     2941 accacgtggt tcagtatggg gcaggcaccg gtcggctgca agacgacgga aggctgcgcc
     3001 caggagtagg cgccagtcga cggagctgcg tgccggcggc atgtccaacg gtcgagagcg
     3061 cggccctcgc tgctagactg cttcacgttc atcggtggga gcggccatgc gcgcgacggt
     3121 gatgatcgtg gtgtccgtcc ttgcgatcgt ttcgacggca aaggcgcaaa catcgacagc
     3181 gacggtgcct tcggcagatg gaacgccgat cgcgttcgag tgcgccggag cgggcccgtc
     3241 gctcgtcatt gtccacggcg gcaccggcga ccgcacgcga tggaccccgc tatttccgtt
     3301 gctcgcagcg cattttcgcg tatgcgcgat ggatcggagg gcgcacggcg cgagcggcga
     3361 tgcgccttcc tacagcctgg ccaaagaagt cgaggacgtt gtcgctgtcg tcgaggcgct
     3421 ctcctcggcg atcactccgc aacgttggcg cgaatggacg aattgatacg cagcggcg
//
LOCUS       DASSNN010000403         5025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_673669, whole genome shotgun sequence.
ACCESSION   DASSNN010000403 DASSNN010000000
VERSION     DASSNN010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673669"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..2036)
                     /locus_tag="VFL84_07510"
     CDS             complement(126..2036)
                     /locus_tag="VFL84_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019018565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7608506.1"
                     /translation="MALLVRFDAVSVTFGEQRVLIAADMAIEPGERVCLVGRNGAGKS
                     TTLKLVTGAQDPDDGAVERPSRLRWSLLEQKLADESQELVRDYVAHGMAGQLARIAEF
                     ERLTAAAPQDRAALREIEALQREIDAAGGWNVAVRVNTVISELDLPAELRMSDLSGGW
                     RRRAALAQALVSNPELLLLDEPTNHLDISTIEWLERTVKNHPGAVLFVTHDRSFVENV
                     ATRIVDIDRGKLRSWPGGYTDYLAQKAKSEADEDRQNALFDKKLAEEEIWVRKGVKAR
                     QTRNEGRVRALEELREIRAGRIKRPRSARVQINESAELSGRKVIEVRNVTHGYGGRQL
                     LKDFSLRVMRGDRIGIIGNNGVGKSTLLKILLGELTPDSGSVKLGTNLEVGYFDQLRR
                     ELDESKTVAEIVGEGREYVTIQGHKKHVVAYLTDFLFPAKRALTPIAALSGGERNRVI
                     LAKLFTRPANLLVLDEPTNDLDVETLEALEERLADYDGTLLVVSHDRYFLDAIVTSTL
                     VFEDDGEVRRHAGGYSDWLERHRALAVVDEPAEKETRDSRDARPRPPPRKLSYKLQRE
                     LDALPDLIAGLEQKVAELRSVVNEPEFYRRPHLEVQDALAALHGAEREVEAAIDRWAE
                     LEEQAAAAGDGG"
     gene            2373..2834
                     /locus_tag="VFL84_07515"
     CDS             2373..2834
                     /locus_tag="VFL84_07515"
                     /inference="COORDINATES: protein motif:HMM:NF018693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608507.1"
                     /translation="MNAAKDWNEDNWGLGIEHEFRSTGRWIKVAMANGFEDSMGDPSY
                     MAGGGIKRRFRFGDNSFYTDLGGVAFLMTRETVNGGKPFPGLLPAATFGFKRVALNVT
                     YLPESVVNDVTHSHKYDPEMHGVFFLQLKLDASLFGLGRRGRLFLAENTAE"
     gene            2944..3726
                     /locus_tag="VFL84_07520"
     CDS             2944..3726
                     /locus_tag="VFL84_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET7608508.1"
                     /translation="MKRIQWSLVGITLVLLANAANAAFLVSEKEVRRQARVEWLGMKR
                     HVPIESNARITKYVECIAGNVLDQLPPETRDAFEWEVVVFDDDEVNAFADPNGKIGVF
                     TGILQIADTPDALAAVIGHEVAHATNGHVMDRARKYARQEVWAMLGGAAIGSSDYVRQ
                     TLAIMSGLPFAREQETEADLIGLQYMANAGFDPRSAVYLWKNMAKMHEANRDKPPEFL
                     STHPSDDTRINNMIKNLTPALVKYNSAREAGRRPACTLSPAR"
     gene            complement(3746..4432)
                     /locus_tag="VFL84_07525"
     CDS             complement(3746..4432)
                     /locus_tag="VFL84_07525"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HET7608509.1"
                     /translation="MRVGILCFGVLAVAATAHAQRNRDDDKAATKRACSVEECFFERD
                     IREFEVVDQTHVIVFTGPQRCAFHVELRGTMCDLTFAPDLDFSRRGDLSSGGFTGAEP
                     VPGGAAADPFDPLESARRNDHALRICSNDLAIQVTGGRFTESATTNVATDRFGNPRTD
                     CQVMTVTSITDDQLVEFYVGRGVLPPLPPMGTGEIEVGEQDEPDEPEAGAADGEAPPA
                     DEPKGRGKRR"
     gene            complement(4506..>5025)
                     /locus_tag="VFL84_07530"
     CDS             complement(4506..>5025)
                     /locus_tag="VFL84_07530"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET7608510.1"
                     /translation="YAEIKDPAWHRRHGVDLSKLVLAQLRAFGYSRAEDAAFVQCFDA
                     AELARLRNELECPLRLIQLVGTEPEYAELLTASGLRSLSAHADGLGPHYSQLVTTEEG
                     RPRPAELARRARDAGLRVHAYTFRRDDLPPYARTLEELLELFFRDVAVDGVFSDHPDV
                     AVRVRAAALEVQ"
BASE COUNT          820 a         1689 c         1651 g          865 t
ORIGIN      
        1 gcgctcgacg cgctcgcggc tcgattcggc cgcggcgtcg tgcagcgcgc cggcgatctc
       61 gcgcgcgacc gcggcgtcgg cgtcgccgcg aatctcgact tcctgcacga ccgcgaaaac
      121 gacgactagc cgccgtcgcc cgcggccgcc gcttgctcct ctagctcggc ccagcgatcg
      181 atcgcggctt cgacctcgcg ctcggcgccg tgcagcgcag cgagggcgtc ctgaacctcg
      241 agatgcggcc gccgatagaa ctccggctcg ttgacgacgg agcgcagctc cgcgactttc
      301 tgctcgagcc ccgcgatcag atcgggcagg gcgtcgagct cgcgctgcag cttgtacgag
      361 agcttgcgcg gcggcggccg cgggcgcgcg tcgcggctgt cgcgtgtctc tttctcggcc
      421 ggctcgtcga cgacggcgag cgcgcgatgg cgctcgagcc aatcgctgta gccgcccgcg
      481 tggcgccgca cttcgccgtc gtcctcgaac acgagcgtgc tcgtgacgat cgcgtccaaa
      541 aagtaacgat cgtggctcac gacgagcagc gtgccgtcgt agtcggcgag ccgttcctcg
      601 agcgcttcga gcgtctcgac gtcgagatcg ttggtcggct cgtcgagcac gagcagattc
      661 gccggccgcg tgaagagctt cgcgagaatc acgcggttgc gctcgccgcc cgacagcgcg
      721 gcgatcggcg tcagggcgcg cttggccgga aacaggaagt cggtgagata agcgacgacg
      781 tgcttcttgt gaccttggat cgtcacgtat tcgcggccct cgccgacgat ctcggcgacg
      841 gtcttgctct cgtcgagctc gcggcgcagc tgatcgaaat agccgacctc gagattggtc
      901 ccgagcttga ccgaccctga gtcaggcgtc agctcgccga gcaggatctt cagcagcgtg
      961 ctcttgccga cgccgttgtt gccgatgatc ccgattcgat cgccgcgcat cactcgcagc
     1021 gagaagtcct tgagcagctg gcggccgccg tagccgtgcg tgacgttgcg cacttcgatg
     1081 accttgcggc ccgacagctc cgcggattca ttgatctgca cgcgcgccga gcgcgggcgc
     1141 ttgatgcgcc ccgcgcggat ctcgcgcagc tcctcgagcg cgcgcacgcg gccttcgttg
     1201 cgagtctggc gcgccttcac gcctttacga acccagatct cttcctcggc gagcttcttg
     1261 tcgaagaggg cgttctgccg gtcctcgtcg gcctcgctct tcgccttctg cgcgagataa
     1321 tccgtgtagc cgcccggcca gctgcgcagc ttgccgcgat cgatgtcgac gattcgcgtg
     1381 gccacgttct cgacgaagct gcggtcgtgc gtgacgaaca gcacggcacc gggatggttc
     1441 ttgaccgtgc gctcgagcca ctcgatcgta ctgatgtcga gatgattagt cggctcgtcg
     1501 agcagcagca gctccgggtt cgacacgagc gcctgagcca gcgccgctcg ccggcgccag
     1561 ccgcccgata agtcgctcat gcgcagctcg gccggcaaat cgagctcgct gatgacggtg
     1621 ttgacgcgca cggcgacgtt ccagcccccg gccgcgtcga tttcgcgctg cagcgcttcg
     1681 atctcgcgca gcgcggcacg gtcctgcggc gcggccgcgg tcagccgttc gaactcggcg
     1741 attcgcgcga gctgtccggc catgccgtgc gcgacatagt cgcggacgag ctcctgcgac
     1801 tcgtcggcga gcttttgctc gagcaggctc caccgcaggc gcgaaggccg ctcgacggcg
     1861 ccatcgtcgg ggtcttgggc gccggtcacg agcttcagcg tagtcgactt gccggccccg
     1921 ttgcggccga ccaaacagac gcgctcgccg ggctcgatcg ccatgtcggc ggcaatcagg
     1981 actcgctgct cgccgaatgt cacggagacg gcgtcgaagc gcaccaaaag ggccatggag
     2041 cacgtaggag ctaaatggga gggcgttact gtagccgcgg ccgacacggg cgcaagcggc
     2101 acggcgacgc cgggtccggc cacgatcctt gagataacat aatatacatt atgcgccaaa
     2161 cggccggacc ggccgtcggc ggagtttaca gatctttcga gtagtcgcgc ctaactcgtt
     2221 gtttcaagcc actggcatgt gtcttgctca tccgaggcat gcgccacgcg acacggattg
     2281 gtctcgccgc tgcactgctg tatctcagcc attcagccgc cgcgcagtcg gacggcggtt
     2341 gggacgttat cctgaacggc cgtgcggttc acatgaacgc tgccaaagat tggaacgagg
     2401 acaactgggg cctcggcatc gagcacgagt tccgctcgac cggtcgctgg atcaaggtcg
     2461 cgatggcgaa cggcttcgag gacagcatgg gcgatccgtc ctacatggcc ggcggcggca
     2521 tcaagcggcg ctttcgtttc ggcgacaaca gcttctacac cgacctcggc ggcgtggcgt
     2581 tcctgatgac gcgcgagacc gtcaacggcg gcaagccgtt cccgggattg ctgcccgcgg
     2641 cgaccttcgg cttcaagcgc gtcgcgttga atgtgacata tctgccggaa agcgtcgtca
     2701 acgacgtcac ccactcgcac aagtacgatc ccgagatgca cggcgtgttc ttcctgcagc
     2761 tgaagctcga cgccagcctg ttcgggctcg gccgccgtgg gcggctgttt ctcgccgaaa
     2821 acacggccga atgagcccgg cggccggtgg ggccgccatt cagttttcct gtgctatctt
     2881 tgcccgaact gggcggctga aggcgcgctc taacggctac gatccatcga cgagggatga
     2941 tcgatgaagc gtattcaatg gtcgctggtt gggatcacgc tggtgctgtt ggcgaacgct
     3001 gccaacgccg cattcttggt ctccgagaag gaagtcaggc gccaggcccg agtcgaatgg
     3061 ctgggcatga agcgccacgt gccgatcgag tccaatgcgc gcatcaccaa gtacgtcgag
     3121 tgcatcgccg gcaacgtgct cgaccagctg ccacccgaga cgcgcgacgc gttcgaatgg
     3181 gaagtcgtcg tgttcgacga cgacgaggtc aacgctttcg cggacccgaa cggcaagatc
     3241 ggcgttttca cgggcatcct gcagatcgcc gacacacctg atgcgttggc agccgtcatc
     3301 ggccacgagg tggcgcacgc gacgaacggg cacgtcatgg atcgagcgcg aaagtacgcg
     3361 cgacaggaag tctgggcaat gctcggcggc gcggccatcg gcagctccga ctacgtgcgt
     3421 cagacgctcg cgatcatgtc gggcctgccg ttcgcgcgcg agcaggaaac cgaggcggac
     3481 ctgatcgggc ttcagtacat ggcaaacgcg gggttcgatc ctcggtctgc cgtctacctc
     3541 tggaaaaaca tggcgaagat gcacgaggcg aaccgcgaca agccgcccga gtttctgtcg
     3601 acgcacccgt cggacgacac gcgcatcaac aacatgatca agaatctgac gcccgcgctc
     3661 gtcaagtaca actcggcgcg cgaggcgggc cggcgccctg cctgcacgct gtcgccggca
     3721 cgctagcttt accgcaaggc cttttttagc gccgcttccc gcggcccttc ggctcgtcgg
     3781 ccggcggcgc ttcgccgtcc gcggcgcccg cttccggctc gtccggctcg tcctgctcgc
     3841 cgacctcgat ttcgccggtt cccatcggcg gcaacggcgg caggacgccg cggccgacgt
     3901 agaactccac gagctggtcg tccgtgatcg acgttaccgt catgacctgg cagtcggtac
     3961 gcgggttgcc gaaccggtcc gtcgctacgt tggtcgtcgc cgattccgtg aatcgaccgc
     4021 ccgtcacttg gatggcgaga tcgttcgagc aaatgcgcag cgcatgatcg ttgcggcgcg
     4081 cgctctcgag cggatcgaac ggatccgcgg ctgcgccgcc ggggacaggc tcggctccgg
     4141 taaacccgcc gctcgacaga tcgccccgac ggctgaagtc gaggtcgggc gcaaacgtga
     4201 ggtcgcacat cgtgccgcgc agctccacat gaaaggcaca ccgctgcggg cccgtgaaga
     4261 cgatgacatg cgtctggtcg acgacttcga actcgcggat gtcgcgctcg aaaaagcact
     4321 cctcgacgct gcaggcccgt ttcgtggccg ctttgtcgtc gtccctattt ctctgcgcgt
     4381 gcgccgtcgc agccaccgcc agcacgccaa agcacaggat gccaactctc atgacgcatc
     4441 ctccttcgct cggtttcact gtaggccagt acggccacga ctgtccacgc cgcaatcggc
     4501 cgcgctcatt gaacctcgag cgccgccgcg cggacgcgca cggcgacgtc gggatggtct
     4561 gaaaatacgc cgtcgacggc gacgtcgcgg aaaaagagct cgagcaattc ttcgagcgtc
     4621 cgcgcgtacg gcggcaagtc gtcgcgccgg aacgtgtacg catgcacgcg cagcccggcg
     4681 tcgcgcgcgc ggcgggcgag ctcggccgga cggggccgcc cctcctccgt cgtgacgagt
     4741 tgcgagtagt gcggcccgag gccgtcggcg tgagcggaca gagaacgcag gcccgacgcc
     4801 gtcagcagct cggcgtactc gggctcggtg ccgacgagct ggatcagccg cagcggacac
     4861 tcgagctcgt tcctgagccg cgcgagctcg gccgcgtcga agcactgcac gaacgcggcg
     4921 tcctctgcgc gtgaatagcc gaatgcgcgc agctgcgcga ggacgagctt cgagagatcg
     4981 acgccgtgcc gccggtgcca ggccggatcc ttgatctctg cgtag
//
LOCUS       DASSNN010000404         5634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_813472, whole genome shotgun sequence.
ACCESSION   DASSNN010000404 DASSNN010000000
VERSION     DASSNN010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813472"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VFL84_07535"
     CDS             complement(<1..1102)
                     /locus_tag="VFL84_07535"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET7608511.1"
                     /translation="MQRSALSLALAAAIVMPAAAQEVETVIVTGSYIRRPSQFDSPSP
                     LVNITADDLAATGANEVGRVLEDLTINTGSQNNPDAFTQNFTTGTTNVNLRGLGVSST
                     LVLINGRRQTQSAVTTDRGENFVDTSSLPPAIAYERIEILKDGATALYGSEAVAGVVN
                     FITRSRFEGFDLELAAQGNTEHPQQDLELSGLWGWGGDATHLLVAFSHLDREPLTTYE
                     RRLSSQTDDLSQAGMPGSFLVPSLPGNPAYRSVWTAAFDGNRNGVADFVEPQLGLPAV
                     PGAQPPVFADQDCIAIAAQDPKVVPAIAAAVPSPIGTIPIGLCEFDFGGFYSIVPEET
                     RSSAFAEVTHAFGDRLEGRLEAHIANNEALRKN"
     gene            complement(1120..3105)
                     /locus_tag="VFL84_07540"
     CDS             complement(1120..3105)
                     /locus_tag="VFL84_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608512.1"
                     /translation="MRSIRRLTLVYLAAAVIVSGCEGGSSDEPQPITFTPAAAPSGTG
                     FVAMYAPPVDNVPYPNDIYNPTGTKLAVPVKITSPLANALNTLDGFSTTAVVSAPFNA
                     PLDPASLIPWNPLSMAPTAASIIVLNATAGTPLVPGADYTVRISAAAGSGGGVLEIVP
                     LKPLAPRTRYAFIVTNGVRNTANVAAGADLVFRAVRDTYLAGGTSVPGQPALDPLFLV
                     IPPLIDAARALGLQGSNVVVAWSMLTQSTTDVLDVVDASTGARPAVLASAGITTAQLG
                     LGLPGIASIYTGYLDVAYYGDPANPLTSFWLNSSLTGPPNVENPTPFARVPNKRIPLL
                     ATLPNNQPQPAAGWPVVIFQHGITLNRTVMFSMADSFAAAGFAVVAIDLPLHGITDTA
                     SPFYQAANERHFNMDNVGAVGVFAPDGQIDNGWQILNVGNPLNARDHIRQAVADTIAL
                     KRTLPTLDFPDGDANPDLDGNRIHYVGISLGSIISGVFLGLDSEVKTATLSSPAGPWT
                     SILTDPQAIDFGAPIRKALAAQGLPQGTVGFDNFVRDLQTVIDPVDPVNYAVDAAASH
                     PIHVIEVLGDTAVPNRPTDYIAQLWGLPSVSTSTPPAPAPPATVEGIVRFKAGAHSSL
                     FNPQPNPAVTQEMQQQTVFYAASGGSQIRINNASVVQ"
     gene            3231..4238
                     /locus_tag="VFL84_07545"
     CDS             3231..4238
                     /locus_tag="VFL84_07545"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET7608513.1"
                     /translation="MKIAVLFGGDSMERDVSIASASQVVGALRSRGHQVIAYDSGRGR
                     LAPADEQRLFAGKIDRRPPDQKSVSSLPTVVSAPDLADVDLVFLAMHGGSGEDGTVQA
                     LLDLAGIPYTGSGKLGSALGWDKDVAKRLFLAAGVPTPDWLMAPISSSAAAQWLGFPL
                     IVKPNGQGSTVGLTLVRTPPELDAALELAGRYDSQVMLERYIDGRELTVGILDDEPLA
                     VGEIVSHTGPIFDYAAKYQPGGAEEIFPADLTPEQTEKIQSLAVRVHRALKLDVYSRV
                     DFRMDAEGRFWCLEVNTLPGLTEASLLPRAAAAVGISFPELCERICRGALERHGAKPK
                     K"
     gene            complement(4240..5091)
                     /locus_tag="VFL84_07550"
     CDS             complement(4240..5091)
                     /locus_tag="VFL84_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7608514.1"
                     /translation="MSIGMDAFRLANGTPAARLGQGTWQMGEDRGTRAAEMEALRTGL
                     DLGLNLIDTAEMYGNGRSEELIAQVVAGRRDQVYIVSKVLPQNAGRSDAIAACEGSLK
                     RLKTDYLDMYLLHWRGSVPLAETLEAFMALRERGSIRHYGVSNFDVDDLEESLTLKGG
                     ADIATDQVLYNLEQRGVEWALLPWSRKHSLPLMAYSPLGSDSRRLRTHPVLKAMAARL
                     GASPSRIALAWLLRQPDIVVIPKASSVDHVRDNHAALSLELSAEDLEKLDRSFPPPKR
                     GTPLAMI"
     gene            complement(5088..5486)
                     /locus_tag="VFL84_07555"
     CDS             complement(5088..5486)
                     /locus_tag="VFL84_07555"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HET7608515.1"
                     /translation="MSSQKRLLLSIAGVSLLAATIAGAVGSHALRFADAQALRSFETA
                     VQFNFFHGLGVIAIVLVGLAGRGGKLRAAAAWLMIAGTLLFCGSIYARALGVSPGVVA
                     AAPYGGVAFMLGWLALAASPWIGDEGGNDV"
BASE COUNT          873 a         1841 c         1996 g          924 t
ORIGIN      
        1 agttcttgcg cagcgcctcg ttgttggcga tgtgcgcctc gagccggcct tcgagcctgt
       61 cgccgaacgc atgtgtgacc tcggcgaacg cgctgctgcg cgtctcctcg ggcacgatcg
      121 agtagaagcc gccgaaatcg aactcgcaga gcccgatcgg gatcgtgccg atcgggctcg
      181 gcacggccgc ggcgatcgcg ggcacgactt tcggatcctg agcggcgatc gctatgcagt
      241 cctggtccgc gaacacgggc ggctgcgcgc cgggcacggc gggcaggccg agctgcggct
      301 ccacgaagtc ggcgacgccg ttgcggttgc cgtcgaacgc ggccgtccag acggagcggt
      361 acgcggggtt gccgggcagc gacggcacga ggaacgagcc cggcatcccc gcctggctca
      421 ggtcgtcggt ctggctcgag agccgccgct cgtaggtcgt cagcggctcg cggtcgaggt
      481 ggctgaacgc gacgagcagg tgcgtcgcgt ctccgcccca accccaaagg cccgagagtt
      541 cgaggtcctg ctgcgggtgc tcggtgttgc cttgcgcggc gagctcgagg tcgaaccctt
      601 cgaagcggct gcgcgtgatg aagttgacga cgccggcgac ggcctccgag ccgtacagtg
      661 ccgtcgcgcc gtccttcaag atctcgatgc gctcgtacgc gatcgccggc ggcagcgacg
      721 acgtgtcgac gaagttctcg ccgcggtccg tcgtcacggc gctctgcgtt tggcggcggc
      781 cgttgatcag cacgagcgtc gagctgacgc ccaagccgcg caggttcacg ttggtcgtgc
      841 ccgtcgtgaa gttctgcgtg aacgcgtcgg ggttgttctg cgagcccgtg ttgatcgtca
      901 ggtcctcgag gacgcgcccg acctcgttcg cgcccgtggc ggcgagatcg tcggccgtga
      961 tgttcacgag cggcgacggc gagtcgaact gcgacgggcg gcggatgtag gagcccgtga
     1021 cgatgacggt ctcgacttct tgtgccgcgg ccggcatgac gatggcggcc gccagtgcca
     1081 aggaaagcgc tgatctttgc atcggtctct ccacgccctt cattgcacga cactcgcgtt
     1141 gttgatccga atctggctgc ccccgctcgc cgcgtagaac acggtttgct gctgcatctc
     1201 ctgcgtcacg gccgggttcg gctgcggatt gaacagcgac gagtgcgcgc ccgctttgaa
     1261 tcgcacgatg ccttcgacgg tcgcgggcgg cgccggcgcc ggcggcgtcg acgtgctgac
     1321 gctcggcaag ccccagagct gcgcgatgta gtcggtcggg cggttcggca cggccgtgtc
     1381 gccgagcacc tcgatgacgt ggatcggatg actcgccgcc gcgtcgacgg cgtagttgac
     1441 gggatcgacg gggtcgatga cggtctgcaa gtcgcggacg aagttgtcga agccgaccgt
     1501 gccttgcggc agaccttgcg cggccagcgc cttgcggatc ggcgcgccga agtcgatcgc
     1561 ctgcgggtcg gtcaggatcg aggtccatgg gccggccggg ctcgagagcg tcgccgtctt
     1621 gacctccgag tcgagcccca ggaacacgcc gctgatgatg ctgccgagcg agatgccgac
     1681 gtagtgaatg cggttgccgt cgagatcggg attcgcatcg ccgtcgggga aatcgagcgt
     1741 cggcagcgtg cgcttcagcg caatcgtatc cgcgacggct tggcggatgt ggtcgcgcgc
     1801 attcagcgga ttcccgacgt tcaagatctg ccagccgttg tcgatctgcc cgtccggcgc
     1861 gaacacgccg acggcgccga cgttgtccat gttgaagtgg cgctcgttcg ccgcttgata
     1921 gaacgggctc gccgtgtccg tgatgccgtg cagcggcagg tcgatcgcga cgacggcgaa
     1981 accggccgcc gcgaacgagt cggccatcga gaacatgacc gtgcgattca gcgtgatgcc
     2041 gtgctggaag atcacgacgg gccagccggc cgcgggctgc ggctgattgt tcggcagtgt
     2101 cgcgagcagc ggaatgcgct tgttcggcac gcgtgcgaac ggcgtggggt tctcgacgtt
     2161 cggcggcccg gtcaggctcg agttgagcca gaagctcgtc agcggattcg ccgggtcgcc
     2221 gtagtacgcg acgtcgaggt agcccgtgta gatgctcgcg atgccgggca agccgagccc
     2281 gagctgcgca gtcgtgatac cggccgacgc tagcacggcc gggcgcgctc ccgtcgacgc
     2341 gtcgacgacg tcgaggacgt cggtcgtcga ttgcgtcagc atgctccacg cgacgacgac
     2401 gttgctgcct tgtagcccga gggcgcgggc cgcgtcgatc agcgggggga tcaccaggaa
     2461 cagcggatcc aacgcgggct ggcccgggac gctcgtgccg cccgcgagat acgtatcgcg
     2521 cacggcgcga aagacgagat cggcgccggc ggcaacgttg gcggtgttgc gcacgccgtt
     2581 cgtgacgatg aacgcgtagc gcgtgcgcgg ggcgaggggt ttcagcggca cgatctcgag
     2641 cacgccgccg ccgctgccgg ccgccgccga gatgcgcacc gtgtaatcgg cgcctggaac
     2701 gagcggcgtt ccggccgtgg cgttcaacac gatgatcgac gcggcggttg gcgccatcga
     2761 cagcgggttc cacggaatca acgacgcggg atcgagcggt gcgttgaacg gcgcgctgac
     2821 gacggccgtc gtcgagaagc cgtcgagcgt gttgagtgcg ttcgccagag ggctcgtgat
     2881 tttgacgggc acggcgagct tcgtgcccgt cggattgtag atgtcgttcg gataggggac
     2941 gttgtctacg ggcggcgcgt acatcgcgac gaagcccgtg ccggacggag cggcggcggg
     3001 tgtgaacgta atcggctgcg gctcatcgga cgagccgcct tcgcaaccgc tgacaatgac
     3061 ggccgctgcc aaataaacga gcgtcaggcg ccggatcgaa cgcatgtccc cctccgctga
     3121 ttcgattccc gggcggggag tatatacaag acagaaacga gcggcccgtc gcgaaagccg
     3181 ccggaacgtt cgagtacaat cgcgagcttc gcaggcggag gtccggcgct ttgaagatcg
     3241 ctgtgttgtt cgggggcgat agcatggagc gcgacgtctc gatcgccagc gcttcacaag
     3301 tcgtcggcgc cttgcgcagc cgcgggcatc aagtcatcgc gtacgactcg ggccgcgggc
     3361 ggctcgcgcc cgccgacgag cagcgcctgt tcgcgggcaa gatcgatcgg cgcccgccgg
     3421 atcagaaatc agtctcgagc ttgccgaccg tcgtgtcggc ccccgacctc gccgacgtcg
     3481 acctcgtgtt tctcgcgatg cacggaggca gcggcgagga tggcaccgtg caagcgctgc
     3541 tcgatctcgc cggcattccg tacacgggct ccggcaaatt gggcagcgcg ctcggctggg
     3601 acaaggacgt cgcgaagcgg ttgttcctcg ccgccggcgt gccgacgccc gactggttga
     3661 tggcgccgat ctcgagctcc gccgcggcgc agtggctcgg ctttccgttg atcgtgaagc
     3721 cgaacgggca gggctcgacg gtcggcctca cgctggttcg cacgccgccg gagctcgacg
     3781 ccgcgctcga gctcgcgggc cgctacgact cgcaagtcat gctcgagcgc tacatcgacg
     3841 gccgcgagct cacggtcggc attctcgacg acgagcccct cgccgtcggc gagatcgtct
     3901 cccacaccgg gccgatcttc gactacgcgg cgaagtatca gccgggcggc gccgaggaaa
     3961 tcttcccggc cgacttgacg cccgagcaga cggagaaaat ccagagcctc gcggttcgcg
     4021 tgcaccgagc gctgaagctc gacgtctaca gccgcgtcga ctttcgcatg gacgccgaag
     4081 ggcgcttctg gtgcctcgag gtcaacacgc tgccggggct gaccgaggca agcctgctgc
     4141 cgcgggccgc cgcggccgtc ggcatcagct tcccggagct gtgcgagcgc atctgccgtg
     4201 gcgcgctcga acggcacggc gccaagccca agaagtgagc tagatcatcg cgagcggcgt
     4261 gccgcgcttc ggtggcggga acgaacgatc gagcttttcc aggtcctccg ccgagagctc
     4321 gagcgacagc gcggcgtggt tgtcgcggac gtgatccacg ctcgacgctt ttggaatcac
     4381 gacgatgtcg ggttgccgca gcagccacgc gagcgcgata cgcgacggcg acgcgccgag
     4441 ccgcgccgcc atcgccttga gcaccgggtg cgtgcggagc cggcgcgagt cgctgccgag
     4501 cggcgagtac gccatcagcg gcagcgagtg cttgcgcgac cacggcagca gcgcccactc
     4561 gacgccgcgc tgctcgaggt tatagagcac ctgatccgtc gcgatgtcgg cgccgccttt
     4621 cagcgtaagc gactcctcga gatcgtcgac gtcgaagttg ctgacgccgt agtgacggat
     4681 cgagccgcgc tcgcgcagcg ccatgaacgc ctcgagcgtc tcggcgagcg ggaccgagcc
     4741 gcgccagtgc agcaagtaca tgtcgagata atcggtcttc aggcgcttca ggcttccttc
     4801 gcaggccgcg atcgcatcgc tgcggcccgc gttctgcggc agcaccttgc tgacgatgta
     4861 gacctggtcg cgccgcccgg cgacgacttg cgcgatgagc tcctcggacc ggccgttgcc
     4921 gtacatctcc gccgtatcga tgaggttgag gccaaggtcg aggcccgtgc gcagcgcctc
     4981 catctcggcc gcgcgggttc ccctgtcctc gcccatctgc cacgtgcctt ggccgagccg
     5041 cgcggcggga gtgccgttcg ccaatcgaaa tgcgtccatg ccgatgctca tacgtcgttg
     5101 cctccttcat ctccgatcca tgggctcgcg gcgagcgcga gccagccgag catgaatgcg
     5161 acgccgccgt acggcgccgc ggcgacgacg ccgggcgaga cgccgagcgc tcgcgcatag
     5221 atgctgccgc agaagagcag cgttcccgcg atcatcagcc acgcggctgc ggcgcggagc
     5281 ttgccgccgc gcccggcaag gccgacgagc acgattgcga tgacgccgag gccgtggaag
     5341 aaattgaact gcacggcggt ctcgaagctg cgcagcgcct gcgcgtcggc gaatctcagc
     5401 gcgtggctgc cgacggcgcc ggcgatcgtc gcggcgagca acgacacgcc ggcgatcgat
     5461 aacagcagtc ttttttggct cgacacggtt ctagcttacg gcatccggct gcgtacgttc
     5521 gcgagttgat gcaggatagg tgccaatgcg aaacgctcat gcgatcggcg cgacggcggc
     5581 gttcctctcg atggcgtttg gcgcgcgcgc cgagctcgcc gacgtcgtca acga
//
LOCUS       DASSNN010000405         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1455932, whole genome shotgun sequence.
ACCESSION   DASSNN010000405 DASSNN010000000
VERSION     DASSNN010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455932"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFL84_07560"
     CDS             complement(<1..416)
                     /locus_tag="VFL84_07560"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET7608516.1"
                     /translation="MKKKNVFYAQSGGVTAVINASACGVIETARKHKNKLGRVYAGRN
                     GILGALTEDMIDTTAESARTIAALKYTPGGAFGSARYKLKGLDKNRAEYERLIEVFKA
                     HDIGYFFYNGGGDSMDTAHKVSQLGEELGLPIVCIGV"
     gene            527..1174
                     /locus_tag="VFL84_07565"
     CDS             527..1174
                     /locus_tag="VFL84_07565"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET7608517.1"
                     /translation="MRIVMLGAPGSGKGTQAQRLQKDYGFPHISTGDLLRKAVADKTP
                     LGLRAKKVMDAGELVSDEIVLGMIKGRLAAPDTKRGFVLDGFPRNTNQADGLDKLLAT
                     LDLKLDCVVLMDVEFDVLMKRLTGRRTCSKTGAVLNIYFSPPAELEACKKAGGELLQR
                     ADDNEDTIRNRLEVYQRQTAPLIDYYANRGLLKTVAATADVDTVYARLKSALGLK"
     gene            complement(1403..2512)
                     /locus_tag="VFL84_07570"
     CDS             complement(1403..2512)
                     /locus_tag="VFL84_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HET7608518.1"
                     /translation="MSAPQEIGWIDDDAALARLVAALEQTSIAAVDTEFVREKTYYPQ
                     LCLIQIGTGEQVACIDCLADLDLRALYARLFDPGFTWVLHSARQDLEVVFRLAGRMPP
                     KLIDTQVAAALTGYPPQVGLEGLLKRTLDVDLGESFARTDWSRRPLPDAALRYALDDV
                     RHLLAAWQKLDAELARLGRREWLREDSERMLAEHPVPDATAVWSRLKGAHGLNFASQC
                     AALALVRWRETAAQRSDRPRRWLLADDALLAIAAALPRDARALAALAESKFIARSAPA
                     ILAAIESRNDAELQAEVRANAIQAVPDKALVKSLQERVREHAAVLGIEPEILATKRDL
                     IGVALNDPPLHLRTGWRARELAPILNGAAPPAPVA"
BASE COUNT          432 a          938 c          900 g          477 t
ORIGIN      
        1 acgccgatgc acacgatcgg aagcccaagc tcctcgccga gctgcgagac cttgtgcgcg
       61 gtgtccatcg agtcgccgcc gccgttgtag aagaagtagc cgatgtcgtg cgccttgaac
      121 acttcgatga gccgctcgta ctcggcgcgg ttcttgtcga gccctttgag cttgtagcgc
      181 gcagagccga acgcgccgcc cggcgtgtac ttgagcgccg cgatcgtgcg agccgactcg
      241 gcagtcgtgt cgatcatgtc ctcggtcagc gcgccgagaa tgccgttgcg gcccgcgtag
      301 acgcgcccga gcttgttctt gtgcttgcgc gccgtctcga tgacgccgca ggcgctcgcg
      361 ttgatgacgg cggtgacacc gcccgattgg gcgtagaaga cgttcttctt tttcacgggg
      421 cctccggcgt tggcgtgagc cctgtgagaa tagcaaaggt ttgacgaaag tcgcgggtag
      481 ccgctagcgt agcggccgtt cgaattcttg cgatggaggt cgttcgatgc gaatcgtcat
      541 gctcggcgcg ccgggctcgg gcaagggcac gcaggcgcag cgccttcaga aagactacgg
      601 gttcccgcac atctcgaccg gcgatctatt gcgcaaggcc gtcgcggaca agacgccgct
      661 cggcctccga gcaaagaaag tcatggacgc gggcgagctc gtctcggacg agatcgtgct
      721 cggcatgatc aagggccgcc tcgcggcgcc ggacacgaag cgcggcttcg tcctcgacgg
      781 cttcccgcgc aacacgaatc aggccgacgg cctcgacaag ttgctggcga cgctcgacct
      841 gaagctcgac tgcgtcgtgc tcatggacgt cgagttcgac gtcctgatga agcgcctgac
      901 gggccgccgc acctgctcga agaccggcgc cgtcttgaac atctacttct ctccgccggc
      961 cgagctcgaa gcctgcaaga aagccggcgg cgagctgctc caacgcgccg acgacaacga
     1021 agacacgatc cgcaaccgcc tcgaggtgta tcagcgtcag acagcgcctc tgatcgacta
     1081 ctacgcgaac cgcggcctgc tcaaaaccgt cgccgccacc gccgacgtcg acaccgtcta
     1141 cgcgcgcctc aagtccgccc tcggcctgaa atagaacgtc gctcgctcgc tcgatgcgcg
     1201 accatcgcgt cgcggcagcg actgtggcac tgcgcgattt gacctcaccg cgtcgcggca
     1261 gcgatcgccg agaggggctc ccgagacacg ccggaagagc gtccctgcgg cttgtcttcg
     1321 cacatccctg tgctcacacg ctctcggaag ccctgctcga cattcactgc cgcgacgcgg
     1381 tgatctgcaa tcgtgcgcct tcttacgcga ctggagcggg cggagcagcg ccgttgagta
     1441 tcggggcgag ctcgcgggcg cgccagccgg tgcgaaggtg cagcggtggg tcgttcagcg
     1501 cgacgccgat caggtcgcgt ttcgtggcga ggatttccgg ttcgatgccg agcacggctg
     1561 cgtgctcacg cacgcgctct tgcagcgact tcacgagcgc tttgtccggc accgcttgga
     1621 tcgcgttcgc gcgcacctcc gcttgcagct ccgcgtcgtt gcgcgactcg atcgccgcca
     1681 ggatggccgg cgcgctgcgc gcgatgaact tcgactccgc aagcgccgcg agcgcgcgcg
     1741 cgtcgcgcgg cagcgcggct gcgatcgcga gcagcgcgtc gtcggccagc agccagcggc
     1801 gaggccgatc gctgcgctgt gccgcggtct cgcgccagcg cacgagcgcc agcgctgcgc
     1861 attgcgaggc gaaattgaga ccgtgcgcgc ctttgagccg cgaccacacc gcggtcgcgt
     1921 ccgggaccgg atgctcggcg agcatgcgct ccgagtcctc gcgcagccat tcgcggcgcc
     1981 cgagcctcgc cagctccgcg tcgagcttct gccacgccgc gagcaagtgc cgaacgtcgt
     2041 cgagcgcgta gcgcagcgcc gcatcgggca gcggccggcg gctccagtcc gtgcgcgcga
     2101 agctttcgcc gagatccacg tcgagcgtgc gcttcagcaa accttcgagc cccacttgcg
     2161 gcggataacc ggtcagcgcg gcggcgacct gcgtgtcgat gagcttgggc ggcatgcggc
     2221 ccgcgagccg gaagacgacc tcgagatcct gccgcgcgct gtgcagcacc cacgtgaagc
     2281 cggggtcgaa caggcgcgca tacagcgccc gaaggtccag gtcggccaga caatcgatgc
     2341 aggcgacttg ctcgcccgtg ccgatttgga tcaggcacag ctgcgggtag tacgtctttt
     2401 cccgcacgaa ctcggtgtcg acggcggcga tcgaggtttg ctcgagcgcg gcgacgagtc
     2461 tcgcgagcgc cgcgtcgtcg tcgatccaac cgatttcttg cggtgcgctc accgtagatc
     2521 ctccccttgg cgcttacgct atcatcgggc ttcataggag cggaatcgtc ctaagtgctg
     2581 cgcctgatcc tcgcgttcgt cgacatcatg ctgcaccggc gaggacccga tagcttgccc
     2641 tcggcgccgt tcttgttctg ggccctgctc gcgctctcga tcctcgccga ctggctcatt
     2701 ctttggctga ccggagcgcc gggacgagtg ttcgccgtaa acttgct
//
LOCUS       DASSNN010000406         4137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_449802, whole genome shotgun sequence.
ACCESSION   DASSNN010000406 DASSNN010000000
VERSION     DASSNN010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449802"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFL84_07575"
     CDS             <1..319
                     /locus_tag="VFL84_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HET7608519.1"
                     /translation="QDRVSRAWVEELMPNIHAVHARFGFMETPDVAEAVRGARQRGLR
                     IDEADCTYFVGWHLVRAKPRSGLAGLKAEVFAYLQRRSTQAAEFFRMPTKRVVMLATE
                     IDI"
     gene            335..868
                     /locus_tag="VFL84_07580"
     CDS             335..868
                     /locus_tag="VFL84_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET7608520.1"
                     /translation="MPPRSLWSCVALASLALLDPAEGAVIDSTANGFTIENSTEVPTD
                     AMTTWKALVDDVGKWWPRDHTWWGDASELTIDARAGGCFCERLGAQQAQHMQVTFVDP
                     GRLLRLTGGLGPLQGMGLHGVLELRLSPGANGGTSITLFYRAGGYTPDDLKAFAPVVD
                     QVQAQQLGGLADHLRKR"
     gene            878..1177
                     /locus_tag="VFL84_07585"
     CDS             878..1177
                     /locus_tag="VFL84_07585"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7608521.1"
                     /translation="MALYIVQFEDKPNVGELRDKLLKSHFEFLDRVKDRVLVPGSMRE
                     VPSDKPLGGLWIIEAKDEAEVRDIFKDDPFWTGGLRAQVRINRWQKAFPDRKVPI"
     gene            complement(1182..2111)
                     /locus_tag="VFL84_07590"
     CDS             complement(1182..2111)
                     /locus_tag="VFL84_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7608522.1"
                     /translation="MRPHWFVSVIAAAAAGVAFGQAAERFQSPWGPADQRGAANRITP
                     QKVLEAKNLITRGAVYQLGQVYESSMPLFGTRHFSLRIPLIFGPMGSNSLTYHDEIVS
                     GELGQIGTQFDGLGHLGVGDTFYNGNNRADFARPEGLTKLGVENVGALVTRGVLVDVA
                     AFKGVARLEGGYEITLADLRGTLEKQRTEVRSGDIVILHTGWGSLWKVDNAAFNASAP
                     GIGLEAAGYLVEREVVLVGADTWSMEVVPNPNPDLAFPVHQLLIPRNGIYIFENLLTE
                     ELARDRVYEFAFFFAPLRLKGAGGSPGNPLAVR"
     gene            complement(2125..2475)
                     /locus_tag="VFL84_07595"
     CDS             complement(2125..2475)
                     /locus_tag="VFL84_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7608523.1"
                     /translation="MTHSDDRLQAFGEFVRAQRRLAQVSQRNLARISGVSDSYLSQIE
                     RGNYRPSPQVVKALATAFGLEPRQLYTMLGFMDEEQNVAPPSVEQAIRLDTRLDAAAK
                     EAMIRVYRSFVGKD"
     gene            2625..3164
                     /locus_tag="VFL84_07600"
     CDS             2625..3164
                     /locus_tag="VFL84_07600"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET7608524.1"
                     /translation="MLFGLLVFGAIVGVVFAFVGIYNRLVAASDKATRAWNDLDALLR
                     QRHDEIPKLLEAFEPHIGADRAVLDRLLEARAAVFAARQTRDADTLSRAEAQLRAAAT
                     AVMARAAAHPSLDASPAFSLMRQRQATLDLEIKDRRDGYNAAVREYNAAIARTPANIV
                     ALVGAFPRLRPLDFERAGP"
     gene            3370..3813
                     /locus_tag="VFL84_07605"
     CDS             3370..3813
                     /locus_tag="VFL84_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7608525.1"
                     /translation="MTQQQKNIRDVLVVLAIALMIGSFAIPAFNGYLERARVSRAVSD
                     IGTMSLKLHQWQRSGHTLPQHLSEAGIASAVDPWGRPYVYLRAADVSRAQLRKDGELV
                     PLNSDFDLYSLGPDGISALALPAAPSQDDVVRAANGAFIGTAANY"
     gene            complement(3788..>4137)
                     /locus_tag="VFL84_07610"
     CDS             complement(3788..>4137)
                     /locus_tag="VFL84_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608526.1"
                     /translation="DGGETKPANLTLLCEYHHRLLHEGAFSIVKEADRSLRFVTADGR
                     TIPRHGYRREDFVDGDGGGTDESPSAEAFRIAAARPDEQRSEVREPRGVYRLKRSRSL
                     TQCADASSSRQCL"
BASE COUNT          689 a         1393 c         1351 g          704 t
ORIGIN      
        1 ccaggatcga gtctcgcgcg cgtgggtcga ggagctgatg ccgaacatcc acgccgtgca
       61 cgcgcgcttc gggttcatgg agactcccga cgtcgccgaa gccgtgcgcg gcgcgcggca
      121 gcgcgggctc cgcatcgacg aggccgactg cacctacttc gtcggctggc acctcgtgcg
      181 cgcgaagccg cgctcgggcc tcgccggcct caaggccgag gtgttcgcgt acttgcagcg
      241 gcgcagcacg caggcggccg agttcttccg catgccgacg aagcgcgtcg tcatgctcgc
      301 gacggaaatc gacatctagt aaggagtgaa gcagatgcct ccgcgctcgt tgtggtcttg
      361 cgttgcactc gcatcgctgg cgcttctcga tccggcggaa ggcgcagtca tcgattcaac
      421 ggcgaacggc ttcacgatcg agaacagcac cgaggtcccc acggacgcca tgacgacctg
      481 gaaggcgctc gtcgacgacg tcggcaaatg gtggccgcgc gatcacacct ggtggggcga
      541 cgcgagcgag ctcacgatcg acgcgcgagc cggcggttgc ttctgcgagc gcttgggcgc
      601 gcagcaggcg cagcacatgc aggtcacgtt cgtcgatccg ggcaggctgc tgcgtctcac
      661 gggcggcctc ggcccgttac aaggcatggg cctgcacggc gtgctcgagc tccggctctc
      721 gcccggcgcg aacggcggca cgtcgatcac gttgttctat cgcgcaggcg gctacacgcc
      781 cgatgatctc aaagcatttg cacccgtcgt cgatcaggtt caggcgcaac agctcggtgg
      841 tctcgccgat catttgcgaa aacgttaggc tattgcgatg gcgctctata tcgtgcagtt
      901 cgaagacaag ccgaacgtgg gggagctgcg cgacaagctc ctgaaatcgc atttcgagtt
      961 cctcgaccgc gtcaaggatc gcgtcctcgt gccgggctcg atgcgcgagg tgccgagcga
     1021 caagccgctc gggggcctct ggatcatcga agccaaagac gaggccgagg tccgcgacat
     1081 cttcaaggac gacccgttct ggacgggcgg gctgcgcgct caagtgcgca tcaatcgctg
     1141 gcagaaggcg ttccccgacc gaaaggtgcc gatctagcgc gtcagcggac cgcgagcggg
     1201 ttgccgggcg agccgcccgc tcccttcaag cgcagcggcg cgaagaagaa cgcgaattca
     1261 tagacgcgat cgcgcgcgag ctcctcggtc aacaggttct cgaagatgta gatcccgttg
     1321 cgcgggatca gcagctggtg cacgggaaac gcgaggtcgg gattcgggtt cggcacgacc
     1381 tccatcgacc aggtgtcggc gccgacgagc acgacctcgc gctcgacgag atatcctgcg
     1441 gcctcgaggc cgatgccggg cgcgctcgcg ttgaacgccg cgttgtcgac cttccacaac
     1501 gagccccagc ccgtgtgcaa gatcacgatg tcgccggagc gaacctcggt tctctgcttt
     1561 tcgagcgttc cgcgcaaatc cgcgagcgtg atctcgtacc cgccttcgag ccgcgcgacg
     1621 cctttgaacg ccgcgacgtc gacgaggacg ccgcgtgtca cgagcgcgcc gacgttctcg
     1681 acgccgagct tcgtgagccc ttcgggccgc gcgaagtcgg cgcggttgtt gccgttgtaa
     1741 aaggtatcgc cgacgccgag gtggccgagg ccgtcgaatt gcgtgccgat ttggccgagc
     1801 tcgccgctca cgatttcgtc gtgatacgtc aaggaattcg agcccatggg cccgaagatc
     1861 agcgggatgc gcaagctgaa atggcgcgtg ccgaagagcg gcatcgacga ctcgtagacc
     1921 tggccgagct gatagacggc gccgcgcgtg atgaggttct ttgcctcgag caccttctgc
     1981 ggcgtgatgc ggttcgccgc gcctcgttgg tcggccggcc cccacggcga ctgaaaccgc
     2041 tccgcggcct ggccgaaagc gacgccggcc gcggcagccg cgattaccga gacgaaccaa
     2101 tgcggacgca ttcgagaacc tccatcagtc cttaccaacg aacgatcgat agaccctgat
     2161 catcgcctct ttcgcggccg cgtcgagccg cgtatcgagc cggatcgcct gctcgacgct
     2221 cggcggggcg acattctgtt cctcgtccat gaacccgagc atcgtgtaca gctgtcgggg
     2281 ctcgagcccg aacgccgtcg ccaaggcctt gacgacttgc ggcgacggcc ggtaattgcc
     2341 gcgttcgatc tggctcaggt acgagtcgga gacgcccgag atccgcgcga gattgcgttg
     2401 cgagacttgc gccaaccggc gctgagctcg cacgaactcg ccaaaggcct gaagccgatc
     2461 gtcgctgtgc gtcatcgtga gccacgccgc cgccgcgcga atttgaagcg attatggcgg
     2521 ggccccccct agcgtcaagc gcgcaaagtc ggatggccac cgaaaggatt tttgtgcaag
     2581 attccgggat tgcgtggcct cgcgatcggc tgctgtcgta gaaaatgctg ttcggtcttc
     2641 tcgtgttcgg tgcgatcgta ggcgtggtct tcgcgttcgt cggcatctat aaccgcctcg
     2701 tcgctgcctc cgacaaagcc acgcgcgcgt ggaacgacct cgacgcgctg cttcgccaac
     2761 gccacgacga gatcccgaag ctgctcgaag cgttcgagcc gcacatcggc gccgaccgcg
     2821 cggtcctcga tcggctgctc gaagcgcgcg ccgcggtctt cgcggcgagg caaacgcgcg
     2881 atgcggacac tctgagccgc gccgaagcgc agctgcgagc cgccgccacg gccgtgatgg
     2941 cgcgcgcggc cgcgcatccc agcttggacg cgagcccagc tttttctttg atgcgccagc
     3001 gacaagcgac gctcgacctc gagatcaagg atcggcgcga cggctacaac gccgccgtcc
     3061 gcgagtacaa cgccgccatc gctcgcactc ccgcgaacat cgtcgcgctc gtcggcgctt
     3121 tcccgcgctt acggccgctc gacttcgagc gcgccggccc gtagcttcaa cgagcacaac
     3181 tccggctcgc gatgctccat aagctgggcg cacgctgcga cggcctcggc gccggcacga
     3241 agcggctcgt cgcctgagcc gcgccgcgcc tcatggatcg aggcatcggc gcttcgtcag
     3301 tgatgcaggt cacagcgatt ccgcgtgcgt tcccccacgc tgctgcgcgc gagcgtttgg
     3361 gggtaaacga tgactcagca gcaaaagaac atacgcgacg tgctcgtggt gctggcgata
     3421 gcgctgatga tcggctcgtt cgcgattccg gccttcaatg gctatctaga gcgtgcgcgc
     3481 gtatcgcgcg ccgtcagcga catcggcacg atgagcctga agctgcatca gtggcagcgc
     3541 agcggccata cgctgccgca gcacctgagc gaggccggga tcgcctccgc cgtcgatccg
     3601 tggggacggc cgtacgtgta cttgcgcgca gccgatgtca gccgcgcgca gcttcgcaag
     3661 gacggcgagc tcgtcccgct gaactccgac ttcgacctct acagcctcgg ccccgacggc
     3721 atctcggccc ttgcgctgcc cgccgcgccg tcgcaggacg acgtcgtgcg cgccgcgaac
     3781 ggcgctttca taggcactgc cgcgaactac tagcgtcggc acattgagtg aggcttctgc
     3841 ttcgcttcag gcgatagacg ccgcgcggtt cgcgtacttc cgatcgctgt tcgtcgggac
     3901 gcgccgccgc gatgcggaat gcctccgcgg agggactctc atcggtgcct ccgccgtcgc
     3961 cgtcgacgaa gtcctcgcgc cgatacccgt gccgcgggat cgtgcgcccg tccgcggtca
     4021 cgaagcgcag tgaccggtcg gcttccttga cgatgctgaa cgccccctcg tgcagcagcc
     4081 ggtggtgata ctcgcacagc aacgtcagat tggcgggctt cgtctcgccg ccgtcga
//
LOCUS       DASSNN010000407         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1084491, whole genome shotgun sequence.
ACCESSION   DASSNN010000407 DASSNN010000000
VERSION     DASSNN010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084491"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="VFL84_07615"
     CDS             <1..951
                     /locus_tag="VFL84_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HET7608527.1"
                     /translation="ARRVVESLQQLAYQFCIIDPEGDYEEQPGAVVNGKPDAEPRRDE
                     VVQLLEDYGVNGVVCLTGVPISDRPSYFSALLTDLLQLRVRYGRPHWLLIDEAHHVFP
                     AEWEIPNALLPETLVNVLLVTVHPELLSREMQQRVTRLVALGPTAGDMVRQFAVLRGI
                     ALPPAAAAAAEADLGVGEVLLLGTADPSCRRVRLEPPSAEQRRHTRKYAVGQLPPDRS
                     FYFRGPEGKLNLRAQNLLQFLEVAAGVDDDTWDHHFRRGDYSRWFRDVIKDPALAAAA
                     EAVERDRFLSRDNALAALRTAVESRYTLPADAPLPVRGAS"
     gene            complement(955..>2703)
                     /locus_tag="VFL84_07620"
     CDS             complement(955..>2703)
                     /locus_tag="VFL84_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7608528.1"
                     /translation="FGFGADEPATYEALMARVHPGDRARVERAVLDATLPDGPRRYTV
                     EFRIVRPDGEVRWISSTGFAVFEHGRLVRVVGTGRNVTERRRVEAALRESEQRFATLA
                     EGSPVLLWVNGPAGHEFVNRAYREFVGVDSDDEVSGYDWSRFVHPDERDDYVNAYQRA
                     FAVQARFTAEFRFRRSDGEYRWMRSEATPRFGDEGELVGYVGAMVDITERRDAEHALR
                     VADRQKDEFLAMLAHELRNPLAPIRNASEVLALRYAGDPEAAVPIAMLRRQSDHLGRL
                     LDDLLDVTRIAQGRVALKQQPLEIGAIVAQAIETVGPLAQAKSQLLRLERDERPLHVS
                     GDRARLVQSVTNVLQNSVKFTRPGGEIVVAVRDAGPDLELTVRDNGCGIAPELVPRVF
                     DLFVQSERTPDRSQGGLGIGLSVVKRLVEMHGGAVSVRSKGLGFGTTVKIRLPRIEPP
                     QSIARPAPAASATRRVLVVDDSTDAADSLAMLLELEGHEVSTAYTAAAALEKAERLQP
                     EVAFIDIGLPQMDGYEVARRLRANQRCRAMRLVALTGYGQPDDRDEARRAGFDHHLVK
                     PADLESVDAILAEPPV"
BASE COUNT          400 a          964 c          917 g          422 t
ORIGIN      
        1 gcgcgccgcg tcgtcgagtc gctgcagcag ctcgcgtacc agttctgcat catcgatccg
       61 gaaggcgact acgaagagca gccgggcgcc gtcgtcaacg gcaagccgga cgccgagccg
      121 cgccgcgacg aggtcgtcca gctgctcgag gactacggcg tcaacggcgt cgtgtgcctg
      181 acgggcgtgc cgatctcgga ccgcccttca tatttttccg ccctgctgac ggatctcttg
      241 cagctgcgcg tacgctacgg gcggccgcac tggctgctca tcgacgaggc gcaccatgtg
      301 ttcccggccg aatgggagat tccgaacgcg ctgctgcccg agacgctcgt gaacgtgctg
      361 ctcgtgacgg ttcatccgga gctcttatcg cgcgaaatgc agcagcgcgt cacgcggctc
      421 gtcgcgttgg ggccgaccgc aggcgacatg gttcggcaat tcgccgtgct gcgcggcatc
      481 gcgttgccgc cggccgcggc cgccgcggcg gaggctgatc tcggtgtcgg cgaggtgctg
      541 cttctcggca cggccgatcc gagctgccgc cgcgtccgcc tcgagccgcc gagcgccgag
      601 cagcgccggc acacgcgcaa gtacgccgtg ggccagctgc cgccggaccg cagcttctac
      661 ttccgcggcc ccgaggggaa gctgaatctg cgggcgcaga acttgctgca gtttctcgag
      721 gtcgcggccg gcgtcgacga cgacacgtgg gaccatcact tccgccgcgg ggattactcg
      781 cgctggtttc gcgacgtcat caaggatccc gcgcttgcgg cggccgccga ggccgtcgag
      841 cgcgaccgct tcttgtcgcg cgacaacgcc ttagccgcgc tgcgcaccgc cgtcgagagc
      901 cgttacacgc tgccggccga cgctccgctg cccgtgcgcg gcgcgagctg aagctcagac
      961 gggcggttcc gcgagtatcg cgtcgacgga ttccaggtcg gccggtttga cgagatggtg
     1021 gtcgaaaccc gcacggcgcg cttcgtcgcg atcgtcgggc tgcccgtagc ccgtgagcgc
     1081 gacgagccgc attgcgcggc agcgctggtt ggcgcgcaac cgccgcgcga cctcgtagcc
     1141 gtccatctgc ggcaacccga tgtcgatgaa cgcgacctct ggctgaaggc gctcggcttt
     1201 ctcgagcgcc gcggcggcgg tatatgccgt cgacacctcg tgcccttcca gctcgagcag
     1261 catcgcgagc gagtcggccg cgtccgtgct gtcgtcgacg accaggacgc ggcgcgtcgc
     1321 gctcgcggcc ggcgccggcc tcgcgatgct ctgcggcggc tcgatgcgcg gcaggcgaat
     1381 cttcaccgtc gtgccaaaac ccaacccttt gctcctgacg ctcaccgcgc cgccgtgcat
     1441 ctcgacgagg cgcttgacga cggacaggcc gatgcccaaa ccgccttgcg accggtcggg
     1501 cgtgcgctcg ctctgcacga acagatcgaa cactcgcggc acgagctcgg gggcgatgcc
     1561 gcagccgttg tcgcgcaccg tcagctcgag gtccggcccc gcgtcgcgca cggcgacgac
     1621 gatctcgccg cccggcctcg tgaacttcac ggagttctgc agcacgttcg tcacgctctg
     1681 cacgaggcgg gcgcgatcgc cgctgacgtg gagcggcctc tcgtcgcgtt cgagccgcaa
     1741 cagctgagac ttggcttgcg cgagagggcc gacggtctcg atggcctggg cgacgatcgc
     1801 gccgatctcg agcggctgct gcttcagcgc gacgcggccc tgcgcgattc gcgtgacgtc
     1861 gagcaagtcg tcgagcaagc gtccgagatg gtccgactgg cggcgcagca tcgcgatggg
     1921 cacggcggcc tcgggatcgc cggcgtagcg caaggcgagc acctcgctcg cattgcgaat
     1981 cggcgcgagc ggatttctga gctcgtgcgc caacatcgcg agaaactcgt ccttctggcg
     2041 gtcggcgaca cgcagcgcgt gctcggcgtc gcgccgctcc gtgatgtcca ccatcgcgcc
     2101 gacgtacccg acgagctcac cctcatcgcc gaaccgcggt gtcgcttccg agcgcatcca
     2161 gcgatactcg ccgtccgagc gtcgaaagcg gaactccgcc gtgaagcgcg cttgcacggc
     2221 gaacgcacgc tggtaagcat tgacgtagtc gtcgcgttcg tcgggatgca cgaaccggct
     2281 ccagtcgtaa ccgctgacct cgtcgtccga atccacgccg acgaactccc gataagcgcg
     2341 attcacgaac tcgtgcccgg cggggccgtt gacccagagc agcacgggcg acccttccgc
     2401 gagcgtggcg aaccgctgct cgctctcgcg caacgccgct tcgacacgcc gccgctccgt
     2461 gacgttgcgg ccggtgccga cgacgcgcac gaggcggccg tgctcgaaaa ccgcgaatcc
     2521 cgtgctgctg atccagcgga cctcgccgtc cggacgcacg atacgaaact ccaccgtgta
     2581 acggcgggga ccgtcgggca gcgtggcatc gaggacggcc cgttcgacgc gtgcgcggtc
     2641 gcccggatga acgcgggcca tgagcgcctc gtaggtcgcc ggctcgtcgg cgccgaaacc
     2701 gaa
//
LOCUS       DASSNN010000408         4017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1820393, whole genome shotgun sequence.
ACCESSION   DASSNN010000408 DASSNN010000000
VERSION     DASSNN010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820393"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /gene="murD"
                     /locus_tag="VFL84_07625"
     CDS             <1..1352
                     /gene="murD"
                     /locus_tag="VFL84_07625"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET7608529.1"
                     /translation="AVRNAAAKHSLIVGLGETGVAVARYLRTSGERVRVIDSRANPPG
                     LAALRAAVPDADISLETLDPRWLEDASRVLLSPGLPYDLPLAVEARRRGLAVIGDIEL
                     FALAARAPVVAVTGSNGKSTVTTLASKILEAQRLRAPAGGNLGPPALDLLKDEADAYV
                     LEISSFQMETTDSLAPVSATVLNVSADHLDRHGTLERYAELKEKLLKAAELAVVNVDD
                     PLVAAMGARHPRVAPFSVQTELKRGYSVVTLASKSGGERWLARNLTPLLRSAELKISG
                     THNEANALAALALTEPLVTDVDAALEALRTFKGLPHRCQRVTERKGVTFVDDSKGTNV
                     GATLAALNGLSGPLVLIAGGLSKGQDLSPLAAGARGKLRAAVLIGTAAAELETVLKPV
                     CETARAGSMADAVARAAALARRGDTVLLSPACASQDMFRDYRERGEQFARAARELPR"
     gene            1349..2500
                     /gene="ftsW"
                     /locus_tag="VFL84_07630"
     CDS             1349..2500
                     /gene="ftsW"
                     /locus_tag="VFL84_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET7608530.1"
                     /translation="MSAVAVKQDRVAFDSLLIAAVVALVAIGTIMVTSASVSIVDGAP
                     LHFLMRHLGALAVGVAGLAFVVIVPTDLWYRLGWLLLLVGLGLLVLVLAPSIGHAVNG
                     SRRWLLVGPITVQASEPARLCLLLYLAGYAVRRSTQLGESLNGLVKPLAVVGVAGVLL
                     LLEPDFGATVVLGATALTVLFVGGARLRDLLLAVLVGGGVLTALAFSADYRVVRLMSF
                     RDPWADPLDTGFQLTQALMAIGRGEWGGVGLGESVQKLFYLPEAHTDFVFAVLVEELG
                     FAGATLVIALFGIVVYRAIMLGREAIESGMHFQGLVATGIGVMLGFEAFVNMGVNSGL
                     LPTKGLPLPLISYGRASTVVTLVALGFLLRIHQELYGGQKRLVQRGYVR"
     gene            2497..3585
                     /gene="murG"
                     /locus_tag="VFL84_07635"
     CDS             2497..3585
                     /gene="murG"
                     /locus_tag="VFL84_07635"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET7608531.1"
                     /translation="MSARPIMIMAGGTGGHVFPGLAVAAELKARQREVVWLGTQRGLE
                     ARLVPQHGIEIEWITIAGVRGRGALAWLSAPFRIAKAVLQALGALRRRRPAAVLGMGG
                     FVAGPGGVAAWLSRLPLIIHEQNSVAGSTNRWLAPLATRVFEAFPGSFPARAHAELIG
                     NPVRRSLTEAAAPRARLLERRGARRRVLVIGGSQGARVVTLTVPKALAQLPADQRPEV
                     WHQAGRGLGDARAAYAEARVDARVVEFIDDMGEAYQWADLVVARSGALTVSELAMVGV
                     GAVLVPFAAAIDDHQTLNARHFAKGGAALVIPELELTPESLARSLTACLADLDGLAEM
                     AEAARAHAKHAVAERIADACVEAAEVRA"
     gene            3582..>4017
                     /locus_tag="VFL84_07640"
     CDS             3582..>4017
                     /locus_tag="VFL84_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HET7608532.1"
                     /translation="MKTRMGRIRNIHFVGIGGAGMAGIAEVLVNLGYAVSGSDVKLTG
                     VTERLRKLGARVAEGHAAENLGDADVVVVSSAVAADNPEIVGANARRIPVVRRAEMLA
                     ELMRFRVGIAIAGTHGKTTTTSLIASVLAEAGLDPTFVIGGRL"
BASE COUNT          522 a         1402 c         1461 g          632 t
ORIGIN      
        1 ctgcggtgcg aaacgccgcg gcgaaacata gtttgatcgt agggctcgga gagaccggcg
       61 tggccgtcgc gcgctatttg cgcacgagtg gcgagcgcgt gcgcgtgatc gacagccgcg
      121 cgaacccgcc cggactcgcc gcactacgtg ccgccgttcc cgatgccgac atctcgctcg
      181 agacgctcga cccgcgctgg ctcgaggatg cgagccgcgt gctgctgtcg cccggcttgc
      241 cttacgactt gccgctcgcc gtcgaggcgc gccggcgcgg cctggccgtc atcggcgaca
      301 tcgagctgtt cgcgctggcc gcgcgggctc ccgtcgtcgc ggtcacgggc agcaacggca
      361 agagcacggt cacgacgttg gcgtcgaaga tactcgaggc gcaacggttg cgcgcgcccg
      421 ccggcggcaa cctggggccg cccgcgctcg atctgctgaa ggacgaagcg gacgcgtacg
      481 tgctcgagat atcgagcttc cagatggaaa cgacggacag cctggcgccg gtctccgcga
      541 cggtcttgaa cgtctcggcc gatcatctcg accggcacgg cacgctcgag cggtacgccg
      601 agctcaagga gaagctgttg aaggcggccg agctcgccgt cgtcaacgtc gacgatccgc
      661 tcgtcgcggc gatgggcgcg cgtcacccgc gcgtcgcgcc gttctcggtg cagacggagc
      721 tcaagcgcgg ctactcggtc gtgacgttgg cttcaaaatc ggggggcgag cgctggctcg
      781 cgcgcaacct cacgccgctg ctgcgctcgg cggagctcaa gattagcggc acgcacaacg
      841 aggcgaacgc gctcgccgcg ctcgcgctga ccgagccgct cgtgaccgac gtcgacgcgg
      901 cgctcgaggc gctgcggacg ttcaagggtc tgccgcaccg gtgccaacgc gtgaccgagc
      961 gcaaaggcgt cacgttcgtc gacgactcga aaggcacgaa cgtcggcgcg acgctcgcgg
     1021 cattgaacgg gctctcgggt ccgctcgtgc tgattgcggg cggcctgagc aaaggccagg
     1081 acttgagccc gctcgccgcc ggagcgcgcg gcaagctgcg ggccgcggtc ctgatcggca
     1141 cggctgccgc cgagctcgag accgtgctga agccggtctg cgagaccgcg cgcgcgggca
     1201 gcatggccga cgccgtcgcg cgcgccgccg cgctggcgcg acgcggcgac accgtgttgc
     1261 tgtcgccggc gtgcgcgagc caggacatgt tccgcgacta tcgcgagcgc ggcgagcaat
     1321 tcgcacgcgc cgcgcgggag ctgccgcgat gagcgccgtc gccgtcaagc aggaccgcgt
     1381 ggccttcgac tcgctgctga tcgcggccgt tgtcgcgctc gtcgcgatcg gcacgatcat
     1441 ggtcacgtcg gcgtcggtat cgatcgtcga cggcgccccg ctccatttcc tgatgcgcca
     1501 cctcggcgcc ttggccgtcg gagtggccgg gctcgcgttc gtcgtcatcg tgccgaccga
     1561 tctgtggtac cgcctgggct ggctgctgct cctcgtcggc ctcgggctgc tcgtgctcgt
     1621 gctggcgccg tccatcgggc atgccgtcaa cggcagccgc cggtggctgc tcgtcggccc
     1681 gatcacggtg caagcgtcgg agccggcgcg gctttgcctg ctgctctatc tcgccggtta
     1741 cgccgtccgg cgctcgacgc agctcggcga gagcttgaac ggcctcgtca agccgctcgc
     1801 cgtcgtcggc gtcgccggtg tgctgctgct gctcgagccc gacttcggag cgacggtcgt
     1861 gctcggcgcg acggcgctga cggtgctgtt cgtcggcggc gcacgcttgc gcgatcttct
     1921 gctcgccgtg ctcgtaggcg gcggcgtctt gaccgcgctc gcgttctcgg ccgactaccg
     1981 cgtggtgcgg ctcatgtcgt tccgcgaccc gtgggccgat ccgctcgaca cgggctttca
     2041 attgacgcag gcgctgatgg cgatcgggcg cggcgagtgg ggcggcgtcg gcttgggcga
     2101 gagcgtgcag aagctcttct acctgcccga ggcgcacacc gacttcgtgt tcgccgtgct
     2161 cgtcgaggag ctcggcttcg cgggcgccac gctcgtcatc gcgctgttcg gcatcgtcgt
     2221 ctatcgcgcg atcatgctcg gccgcgaggc gatcgagagc ggaatgcact tccagggcct
     2281 cgtcgcgacg ggcatcggcg tcatgctcgg cttcgaagcg ttcgtcaaca tgggcgtgaa
     2341 ttccggcctg ctgcccacga agggactgcc gctgccgctg atcagctacg gccgcgcgag
     2401 cacggtcgtc acgctcgtgg cgctcggctt cctgctgcgc attcatcaag agctctacgg
     2461 cggccagaag cgcctcgtgc agcgaggcta cgtgcgatga gcgcgcggcc gatcatgatc
     2521 atggcgggcg gcacgggcgg gcacgtcttc ccgggcctcg ccgtcgcggc cgagctcaag
     2581 gcgcggcagc gcgaggtcgt ctggctcgga acgcagcgcg ggctcgaagc gcggctcgtg
     2641 ccgcagcacg gcatcgagat cgagtggatc acgatcgcgg gcgtccgcgg ccgcggtgcg
     2701 ctggcgtggc tgtcggcgcc gtttcgaata gccaaagccg tgctccaggc gctcggtgcg
     2761 ctgcgccgcc gccggcccgc ggccgtgctc ggcatgggcg ggttcgtcgc cggtcccggc
     2821 ggcgtcgccg cgtggctctc gcggctgccg ctgatcatcc acgagcagaa ctccgtcgcc
     2881 ggctcgacga atcgttggct cgcgccgctc gccacgcgcg tgttcgaggc gttcccgggc
     2941 agcttcccgg cgcgggcgca cgccgagctg atcggcaatc cggtgcgccg ctcgctgacc
     3001 gaagctgcgg cgccgcgtgc gcggctgctc gagcgcagag gcgcgcgccg ccgcgtgctc
     3061 gtgatcggcg gaagccaggg cgcgcgggtc gtgactctga cggtgccgaa agcgctcgcg
     3121 cagctgcctg cagaccagcg tcccgaggtc tggcatcaag caggccgcgg gctcggcgac
     3181 gcgcgcgccg cgtacgcgga ggcgcgcgtc gatgcgcgcg tcgtcgagtt catcgacgac
     3241 atgggcgagg cctatcagtg ggccgacctc gtcgtcgcgc gctccggagc gttgacggtt
     3301 tccgagctcg cgatggtcgg cgtgggcgcg gtcctcgtgc cgttcgcggc cgccatcgac
     3361 gaccaccaga cgctgaacgc gcgtcatttc gcgaaaggcg gcgccgcgct cgtgatcccg
     3421 gagctcgagc tgactccgga aagcctcgct cgctcgttga ccgcctgcct cgccgatctc
     3481 gacggtctcg ccgagatggc ggaggctgcg cgcgcgcacg cgaagcatgc cgtggccgag
     3541 cggatcgccg acgcctgcgt cgaggccgcg gaggtgcgcg catgaagacg cgcatgggcc
     3601 gcatccgcaa cattcacttc gtcggcatcg gcggcgccgg aatggcgggc attgccgagg
     3661 ttctcgtgaa tctcggctac gccgtcagcg gctcggacgt gaagctcacc ggcgtgacgg
     3721 agcggctgcg caagctcggc gcgcgcgtcg ccgaagggca cgcggccgag aacctgggcg
     3781 acgccgacgt cgtcgtcgtc tcgagcgccg tggcggccga caatcccgag atcgtcggcg
     3841 cgaacgcgcg ccgtatcccc gtcgtgcgcc gcgccgagat gctcgccgag ctcatgcgct
     3901 tccgcgtggg catagcgatc gccggcacgc acggcaagac gacgacgacg agcttgatcg
     3961 cgagcgtgct ggccgaagcg ggcctcgacc cgacgttcgt aatcggcggc cggctcg
//
LOCUS       DASSNN010000409         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1687709, whole genome shotgun sequence.
ACCESSION   DASSNN010000409 DASSNN010000000
VERSION     DASSNN010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687709"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /gene="glmM"
                     /locus_tag="VFL84_07645"
     CDS             <1..893
                     /gene="glmM"
                     /locus_tag="VFL84_07645"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET7608533.1"
                     /translation="SAAREDYVEFCKSTIPEGMGFEDFKIVVDASHGAAYKVAPRVMS
                     DLGADVVPVGCSPNGRNINDGCGSTRPDLLKLTVTGVRAHVGVALDGDGDRVVMVDER
                     GSLVDGDQLLYILARDRSSSNELRGPVVGTVMSNLGLELALRGLGIDFERAKVGDRHV
                     LALLKSKGGILGGETSGHIICLDRTTTGDGLITALQVLSVMRRTGLRLSELVKGMEQF
                     PQVLLNVKVREPVDPTRDPAVREAVGRAESELGSRGRVVLRASGTEPVIRVMVEGEDR
                     QQVDALAGQLAESVRSTFGR"
     gene            1118..1876
                     /gene="tpiA"
                     /locus_tag="VFL84_07650"
     CDS             1118..1876
                     /gene="tpiA"
                     /locus_tag="VFL84_07650"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET7608534.1"
                     /translation="MTRKPLIAGNWKMHGSLAESRQLVEALRAGIDPRAPATMLVCPP
                     YVYLPAVQTWLQGSPILLGAQDVADKPGTGAYTGEVSGQMLRDVGCSHVIVGHSERRA
                     LYGETDAVVAAKFKAAQAAGLTPIVCVGETLEQREAGQTRSIIQKQVTAVVDSAGVHA
                     FAKAVIAYEPVWAIGTGRTASPEQAQEVHAFIRGMIAARDDTIAAGLNILYGGSVKGA
                     NARSLFAMADIDGGLVGGASLVAAEFLEIFRAAA"
     gene            1894..2256
                     /gene="secG"
                     /locus_tag="VFL84_07655"
     CDS             1894..2256
                     /gene="secG"
                     /locus_tag="VFL84_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET7608535.1"
                     /translation="MLQTIVLVAHVGIALLIIGLVLLQRGKGADAGTGFGAGASGTVF
                     GARGSATFFSRVTGVLAALFFVTSLTLAWLATQRTAPTSLLELAPAVETTTTPPASET
                     PPAAVEPPSDAPAQQPNE"
     gene            2269..2353
                     /locus_tag="VFL84_07660"
     tRNA            2269..2353
                     /locus_tag="VFL84_07660"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2303..2305,aa:Leu,seq:gag)
BASE COUNT          399 a          778 c          828 g          406 t
ORIGIN      
        1 catcggcggc gcgcgaggac tatgtcgagt tctgcaaatc cacgatccca gagggaatgg
       61 ggttcgagga tttcaagatc gtcgtcgatg cgtcgcacgg cgccgcgtac aaggttgcgc
      121 cgcgcgtgat gtcggatctc ggcgccgacg tcgtgccggt gggttgctcg ccgaacggcc
      181 gcaacatcaa cgacggctgc ggctcgacgc ggcccgattt gctgaagctg accgtcacgg
      241 gtgtgcgcgc gcacgtcggc gtggcgctcg acggcgacgg cgatcgcgtc gtcatggtcg
      301 acgagcgcgg cagtctcgtc gacggcgatc agctgctcta tatcttggcg cgcgaccgct
      361 cgagctcgaa tgagctccgc ggtcccgtcg tcggcaccgt catgagcaac ttggggctcg
      421 agctcgcgct tcgcggcttg gggatcgact ttgagcgcgc gaaagtcggc gatcgtcacg
      481 tgctcgcgtt gctgaagtcg aagggcggaa tcctcggcgg cgagacttcg ggtcacatca
      541 tttgtctcga ccgcacgacg accggcgacg gtctcatcac ggcgttgcag gtgctgtcgg
      601 tcatgcggcg cacggggctt cgcttgagcg agctcgtgaa gggcatggag cagttcccgc
      661 aggtgctgct gaacgtcaag gtacgcgagc ccgtggaccc gacgcgcgac cctgccgtgc
      721 gcgaggccgt cggccgcgcc gagtcggagc ttgggtccag aggccgagtc gtgctgcgcg
      781 cctcgggcac ggagcccgtc atccgcgtta tggtcgaggg cgaggaccgc caacaggtcg
      841 acgcgctcgc agggcagctc gcggagtccg tccgctcgac cttcggccgc tgaccggccg
      901 ccgcgaccga aaaggggacc gaccgaaaag gggacagatc tatttacgac cgaaaagggg
      961 acagatctat ttaagcgccc ccctgacaga ttgatcttct tacgttggtt agggcgctta
     1021 aatagatctg tccccttttc ggcgttgcgt tgcggcgcga cggcccgtaa gctgccgccc
     1081 ccgctggcag caacgccctt ttcaccgcga gactcgcgtg acgcgtaagc cattgattgc
     1141 cggtaactgg aagatgcacg gctcgctcgc cgagagccgg cagctcgtcg aggcattgcg
     1201 ggccggcatc gatccccgcg cgccggccac gatgctggtg tgcccgccgt acgtctatct
     1261 gccggcggtc caaacctggt tgcaaggaag cccgatcctg ctcggcgcgc aagacgtcgc
     1321 cgacaagccc gggaccggcg cgtacacggg cgaggtgtcg ggccagatgc tgcgcgacgt
     1381 cggctgcagc cacgtcatcg tcggccattc cgagcgccgc gcgctctacg gcgagaccga
     1441 cgccgtcgtc gcggcgaaat tcaaggccgc gcaggcggcc gggctcacgc cgatcgtctg
     1501 cgtcggcgaa acgctcgagc aacgcgaggc gggtcagacg cgctcgatca tccaaaagca
     1561 agtcacggcc gtcgttgact cggccggcgt gcacgcgttc gccaaagccg tcatcgctta
     1621 cgagcccgtc tgggccatcg gcaccggacg caccgcgagc ccggagcaag cccaggaagt
     1681 gcacgccttc atccgcggca tgatcgcggc gcgagatgat acaattgcgg ccggtttaaa
     1741 cattctgtac ggcggcagcg tcaaaggcgc caacgcccga agcttgttcg cgatggcaga
     1801 catcgacggc gggctcgtcg gcggcgcttc actcgtcgcg gcggaattcc tcgagatctt
     1861 ccgcgccgcg gcttaacgaa aaagcagttc gaaatgttgc agacgatcgt attggttgcc
     1921 cacgtgggca tcgcgttatt gatcatcggc ctcgtgctgt tgcagcgcgg caaaggcgcc
     1981 gacgcgggca ccgggttcgg tgccggcgca tcgggcaccg tattcggcgc gcgcggatcc
     2041 gcgacgttct tctcgcgcgt gacgggcgtg ctggccgcat tgtttttcgt gacgagcttg
     2101 acgctggcgt ggctcgcgac gcagaggacc gcgccgacga gtctgctcga gctggctccc
     2161 gcggtcgaaa ccacgacgac tccgccggcg agcgagactc cgccggctgc ggtcgagccg
     2221 ccgagcgacg cgcccgcgca gcagccgaac gagtgactga agcaatcagc ccacgtggtg
     2281 gaattggtag acacgctatc ttgagggggt agtggcgaaa gctgtgcgag ttcgagtctc
     2341 gccgtcggca ccagatcgat ccgccagggc ctcatccggc ctcctggcgg ccgggcgaat
     2401 ccagcgccgg c
//
LOCUS       DASSNN010000410         3640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_73652, whole genome shotgun sequence.
ACCESSION   DASSNN010000410 DASSNN010000000
VERSION     DASSNN010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73652"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..627)
                     /locus_tag="VFL84_07665"
     CDS             complement(160..627)
                     /locus_tag="VFL84_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HET7608536.1"
                     /translation="MKETVLDVLMYLFESYVDSPDEPEPNRHELKRELGRAGFHDREI
                     ERALDWLDGLNSTDVGSAPQNTAFRVFDIVEQERLDAASRGYLLHLEQIGILKAAQRE
                     IVIDRLLALDNEEIDVEQTKWVVMMVLFSQPGEQDAFAQMEDLVYADDSAWVH"
     gene            complement(627..1748)
                     /gene="dprA"
                     /locus_tag="VFL84_07670"
     CDS             complement(627..1748)
                     /gene="dprA"
                     /locus_tag="VFL84_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET7608537.1"
                     /translation="MRVDAWLTLLQAAQQITTDTWLRALKHCGSAEALLAEAPRLFAG
                     SESGAEALAKLSSPDKAKIDRWRAWLDGPHRALITFGSRDYPKRLAEIPDAPLALWAE
                     GRERRLLAKPQLAIVGSRSPTADGRETAEQFARYIAQRGLTITSGLATGIDGASHRGA
                     LAAPGGTIAVLGSGLDTIFPREHTSLAADIAQRGVLVSEYAPGVEAQKLFFPQRNRII
                     AGLTLGTLVVEATRRSGSLITARFALDYGREVFAIPGSIHSPLARGCHHLIRQGAKLV
                     EEAADVLDELAPLLAAEGVELAPEPAVETTDGAPATRDPSYEKLLSELGFSPTPIGDL
                     STRTGLTTAELSSMLLLLELEGQVEALPGGRYSRRGKKN"
     gene            complement(1782..2915)
                     /locus_tag="VFL84_07675"
     CDS             complement(1782..2915)
                     /locus_tag="VFL84_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET7608538.1"
                     /translation="MFFARKPLVRSTTLLAACAASFILLSSGIAQQRDSTLEVAAVGQ
                     QPPSPVLNPRHPDSYVVQRGDTLWDIASMFLRDPWYWPEIWQINPQVENPHLIFPGDT
                     LRLAYLDDGRPAINVERAPLVTDAGGGGFERLSPRVRSTPLDEAINTIPYETIKSFLS
                     RPRFIEKRQIDDMPYVVALREALIGSAGHDVYVRGFEREAPVGTVFNVVELGGAFVDP
                     DTHDVLGYQGTYVGQGRLDRAGDPGTLRLLETEREALVGDLLAEEEDAVPLDFIPRAP
                     ESEVEGKIIAVMSGVSLIGQYAVVVINRGSAAGLEPGHVLRAYQTGQTIRDTQRGLVG
                     QKVRLPDEPAGTMMVFRTAERLSYALVMEATTPLAVYDAVRNP"
     gene            3104..>3640
                     /gene="def"
                     /locus_tag="VFL84_07680"
     CDS             3104..>3640
                     /gene="def"
                     /locus_tag="VFL84_07680"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET7608539.1"
                     /translation="MARLPILEYPDPRLRIRAEPVTKFDTDLKTLVADMFETMYAAPG
                     IGLAATQVDVHKRILVCDVGVESKQPLCLINPEIVRAEGKTSAEEGCLSVPEFYDFID
                     RAASIRVRAFDPDGKPFELDAEGLLAVCIQHEMDHLEGKLFVDYLSELKRERLKKKAA
                     KKAKRAGGEADTRQPTSAP"
BASE COUNT          612 a         1188 c         1218 g          622 t
ORIGIN      
        1 tagctcaaca gcgcgagggc acggctcgcg gcgatgtcgg ttgcgcaggc atggaatgca
       61 gcagactggc ctcgtctttc gcgatgggcg attttaaagg accggcagga tagccgattc
      121 gcgcggcggc gttaaagacg gactcgcagg aactgcgacc tagtgcaccc aggccgagtc
      181 gtcagcgtag acgagatcct ccatctgcgc gaaggcgtct tgctcgcccg gctggctgaa
      241 gagcaccatc atgacgaccc acttcgtctg ctcgacgtcg atctcctcgt tgtcgagcgc
      301 caacaggcga tcgatgacga tctcgcgttg cgccgccttc agaattccga tctgctcgag
      361 gtgcagcagg tagccgcgcg aggcggcgtc gaggcgctct tgctcgacga tgtcgaagac
      421 acgaaacgcc gtgttttgcg gcgcgctgcc cacgtccgtc gagttcaagc cgtcgagcca
      481 atcgagcgcg cgctcgatct cgcgatcgtg gaagccggcg cggccgagct cgcgcttgag
      541 ctcgtgacgg ttcggctccg gctcatcggg ggaatcgacg taggactcga acaggtacat
      601 cagtacgtcg aggacggttt ctttcatcag ttcttcttgc cgcggcggga atatcggccg
      661 ccgggcaacg cctcgacttg gccttcgagc tccagcagca ggagcatgga ggaaagctcc
      721 gccgtcgtca aaccggtcct cgtgctcaaa tcgccaatcg gcgttggcga aaaaccgagc
      781 tcgctcaaca gcttctcgta gctcggatcg cgcgtggccg gcgccccgtc cgtcgtctcg
      841 acggccggtt cgggagcgag ctcgacgccc tcggcagcga gcagcggcgc gagctcgtcg
      901 agcacgtcgg ccgcttcttc tacgagcttc gcgccttggc ggatcagatg gtggcagccg
      961 cgcgcgagcg gactgtggat cgagcccggg atcgcgaaca cctcgcggcc gtagtcgagc
     1021 gcgaaacgcg ccgtgatcaa cgagccgctg cgccgcgtcg cctcgacgac gagcgtgccg
     1081 agcgtgagcc ccgcgatgat gcgattgcgc tgcgggaaga acagcttttg cgcctcgacg
     1141 ccgggcgcgt actccgacac gagcacgccg cgctgcgcga tgtcggccgc gagcgaggtg
     1201 tgctcgcgcg gaaagatcgt gtcgagaccg ctgccgagca cggcgatcgt gccgccgggc
     1261 gcggccaggg cgccgcgatg gctcgcgccg tcgatgcccg tcgcgagccc gctcgtgatc
     1321 gtcaatccgc gttgcgcgat gtagcgcgcg aattgctcgg ctgtttctct tccgtccgcg
     1381 gttgggctgc ggctgccgac gatcgcgagc tgcggcttcg cgagcagccg gcgctcgcgg
     1441 ccttcggccc agagcgcgag cggcgcatcc ggaatttcgg cgaggcgctt cggataatcg
     1501 cggctcccga atgtgatcaa cgctcgatgc ggaccgtcga gccacgcgcg ccagcgatcg
     1561 atcttcgctt tgtccggcga ggaaagcttc gcgagcgctt ccgcgccgga ctcgctacct
     1621 gcgaagagac gcggcgcttc ggcgagcaac gcttcggcgg agccgcagtg cttcagcgcg
     1681 cggagccagg tgtcggtcgt gatctgctgc gccgcttgca gcagcgtcag ccaggcgtct
     1741 acgcgcacgg ccgcgccggc cgccgcgctg tgctgctctc gctacgggtt gcgaacggcg
     1801 tcgtacaccg cgagcggcgt ggtggcttcc atgacgagcg catagctcag gcgttccgca
     1861 gtacggaaca ccatcatcgt gcccgcgggc tcgtccggca atcgcacttt ctggccgaca
     1921 aggccacgtt gcgtatcgcg tatcgtctgt ccggtctggt atgcgcgcag cacgtgcccg
     1981 ggctcgaggc cggccgccga gccgcggttg atgacgacga cggcgtattg gccgatcagc
     2041 gaaacgccgg acatcacggc aatgatcttg ccctcgacct cgctctccgg agcgcgaggg
     2101 atgaaatcga gcggaacggc gtcttcctcc tcggcgagca aatcgccgac gagcgcctcg
     2161 cgctcggttt cgagcagccg cagcgttccc ggatcgcccg cgcgatcgag gcggccttgg
     2221 ccgacgtagg tgccttggta gccgagcacg tcatgtgtat cggggtcgac gaacgcgccg
     2281 ccgagctcga cgacgttgaa cacggtgccg accggcgctt ctcgctcgaa gccgcgcacg
     2341 taaacgtcgt ggcccgcgct gccgatcaac gcttcgcgaa gcgcgacgac gtacggcatg
     2401 tcgtcgatct gtctcttctc gatgaaacgc ggccgcgaca agaacgattt gatcgtctcg
     2461 tacggaatcg tgttgatcgc ctcgtcgagc ggcgtcgagc gaacgcgcgg cgacaggcgc
     2521 tcgaagccgc cgccgccggc gtcggtgacg agcggcgcgc gctcgacgtt gatcgcgggg
     2581 cggccgtcgt cgagataggc gagcctcagc gtgtcgccgg ggaagatcag gtgcgggttc
     2641 tcgacctgcg gattgatctg ccaaatctcg ggccagtacc acgggtcgcg caggaacatc
     2701 gacgcgatgt cccagagcgt gtcgccgcgc tgcacgacgt agctgtcggg gtggcgaggg
     2761 ttcaggacgg ggctcggcgg ctgctggccg accgccgcga cctcgagcgt cgagtcgcgc
     2821 tgctgcgcga tcccgctcga cagcaagatg aagctggccg cgcaggcggc gagcagggta
     2881 gtcgatcgga cgagcggctt gcgcgcgaaa aacatcgtat gactcccgta agacccgtcg
     2941 ttgcgaggcc ggccccactg gctccggctg ctataatccc cgtgcgcttc cgttgcgctc
     3001 gtagcgcttg ccggcccggg tcggaactct cactatccct gtaaagtcag tgagttaagc
     3061 aaacttcgca acgcgttata cacgaaacca aggccgaacc acaatggcac gccttccaat
     3121 actggagtat ccggaccctc gcctcagaat tcgcgcggag ccggtcacaa aattcgatac
     3181 cgacctcaaa actctcgtcg cggacatgtt cgagaccatg tacgcagcgc ccgggatcgg
     3241 gctcgccgcc acccaggtcg acgtccataa gcgcatcctt gtctgcgatg tcggcgtcga
     3301 gagcaagcag ccgttgtgcc tgatcaaccc cgaaatcgtg cgcgccgagg gcaagactag
     3361 cgcagaggaa gggtgtcttt ccgtgccgga gttctacgac ttcatcgatc gcgccgcgtc
     3421 gattcgcgtc cgcgcgttcg atcccgacgg caagccgttc gagctcgacg ccgagggcct
     3481 gctggcggtt tgcatccagc acgagatgga tcatttggaa ggcaagctgt tcgtcgacta
     3541 tctctccgag ctcaagcgcg agcgtttgaa gaagaaggcc gccaagaagg cgaagcgggc
     3601 gggcggcgag gccgataccc gtcagccgac ctccgcgccc
//
LOCUS       DASSNN010000411         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_202466, whole genome shotgun sequence.
ACCESSION   DASSNN010000411 DASSNN010000000
VERSION     DASSNN010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202466"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1906
                     /locus_tag="VFL84_07685"
     CDS             116..1906
                     /locus_tag="VFL84_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020396722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HET7608540.1"
                     /translation="MTTARFVLSLALLLPALAGANEEIERLTGDANNWATWGGNYAGT
                     RYSSLAQIDKSNVANLKVAWTFSTGVLRGHEGGPLVVGDTLYIHTPFPNKVYSINLAD
                     QTINWAYAPNQNADTIPVMCCDTVNRGLAYGDGKIFLQQADTTLVALDAKTGQVAWSV
                     KNGSPAKGETNTNAPLVVKDKVITGISGGEYGVRGFVVAYDIKTGRQIWKAYSVGPDS
                     DLLVDPRRTTHLGRPIGANSSLSTWEGEQWRLGGGTTWGWFTYDPALNLVYYGSGNPG
                     TWNPVVRPGDNRWSMTLFARDADTGMAKWVYQMTPHDEWDYDGVNENVLVDLEVGGRM
                     RKALVHFDRNGFGYTLDRETGALLVAEKFDPAVNWATKVDLRTGRPQVVDHYSTHKNG
                     QDFNTEDICPAALGSKDQQPAAFSPRTKLFYVPTNHVCMDYEPYEVEYVAGQPYVGAV
                     LTMFPAGQATKDGTTHLGNFIAWDAAQGKIVWSTPEPFSVWSGALATGGDVVFYGTLE
                     GYLKAVDAATGKELFRFKTPSGIIGNVNTWTHNGKQYVGVLSGIGGWAGIGMAAGLEG
                     ATDGLGAVGAYRSLARHTQLGGVLTVFALP"
BASE COUNT          386 a          669 c          662 g          302 t
ORIGIN      
        1 gccgattcct ttctccctgc caaacgcgca gaaaatagat ctgtcccctt taccaaggcc
       61 aagcgacaga gctggtttct agacccgacc caatccattc ataaggagag cgggaatgac
      121 cacggcgcgc tttgttctat ccctcgcact gctgttgccg gcgctcgccg gcgccaacga
      181 agaaatcgaa aggctgacgg gcgacgcgaa caactgggcg acgtggggcg gcaactacgc
      241 cggcacgcgc tacagctcgc tcgcccagat cgacaagagc aacgtcgcga acttgaaggt
      301 cgcatggacg ttctcgacgg gagtgttgcg cggccacgaa ggcggcccgc tcgtcgtcgg
      361 cgacacgctg tacatccaca ctccgttccc gaacaaggtc tactcgatca acctggccga
      421 ccagacgatc aactgggcgt acgcgcccaa tcagaacgcc gacacgatcc ccgtgatgtg
      481 ctgcgacacc gtgaatcgcg gcctcgcgta cggcgacggc aagatcttct tgcagcaagc
      541 cgacaccacg ctcgtcgcgc tcgacgcgaa gacggggcag gtcgcgtgga gcgtcaagaa
      601 cggcagtccc gcgaaaggcg agaccaacac gaacgcgccg ctcgtcgtca aagacaaagt
      661 catcacgggc atcagcggcg gcgagtacgg cgtgcgcggg ttcgtcgtcg cgtacgacat
      721 caagacgggc cgccagatct ggaaggcgta cagcgtcggt cccgatagcg acctgctcgt
      781 cgaccctcgc cgcacgactc acctcggccg tccgatcggc gcgaactcga gcttgagcac
      841 gtgggaaggc gagcaatggc gcctcggcgg cggcacgacg tggggctggt tcacgtacga
      901 ccccgcgctg aatctcgtct actacggcag cggcaatccc ggcacctgga accccgtcgt
      961 gcgccccggc gacaaccgct ggagcatgac gctgttcgcg cgcgacgcgg acaccggcat
     1021 ggcgaagtgg gtctatcaga tgacgccgca cgacgagtgg gactacgacg gcgtcaacga
     1081 gaacgtgctc gtcgatctcg aagtcggcgg ccgcatgcgc aaggcgctcg tgcacttcga
     1141 tcgcaacggt ttcggctaca cgctcgatcg cgagacgggc gcgctgctcg tcgccgagaa
     1201 gttcgatccg gccgtgaact gggcgacgaa ggtcgacctg cgcacgggcc gcccgcaagt
     1261 cgtcgaccac tacagcacgc acaagaacgg ccaggacttc aacaccgagg acatttgccc
     1321 agccgcgctc ggctcgaagg atcaacagcc ggccgcgttc tcgccgcgca cgaagctgtt
     1381 ctacgtgccg acgaaccacg tctgcatgga ctacgagccg tacgaggtcg agtacgtcgc
     1441 gggccagccg tacgtcggcg ccgtgctcac gatgttcccg gccgggcaag ccacgaagga
     1501 cggcacgacg catctcggca acttcatcgc gtgggacgcg gcgcaaggca agatcgtctg
     1561 gtctacgccc gagccgttct cggtctggag cggcgcgctc gcgacgggcg gcgacgtcgt
     1621 gttctacggc acgctcgagg gctacttgaa ggccgtcgac gcggcgacgg gaaaagagct
     1681 gttccgcttc aagacgccgt cgggaatcat cggcaacgtc aacacgtgga cgcacaacgg
     1741 caagcagtac gtcggcgtgc tgtccggcat cggcggctgg gccggcatcg gcatggcggc
     1801 ggggctcgaa ggcgcgacgg acggcctcgg cgccgtcggc gcttaccgct ctttggcgcg
     1861 ccacacgcag ctcggcggcg tgctgacggt gtttgcgctt ccttagactc aagtccaagc
     1921 gatgagcgcg agagggacgt tcgcgttggg tttcgtagcg gcggcgctcg tcctagtggc
     1981 gagcgccggc cgcgcgcaga cggcggccga cgccgcgcc
//
LOCUS       DASSNN010000412         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1203965, whole genome shotgun sequence.
ACCESSION   DASSNN010000412 DASSNN010000000
VERSION     DASSNN010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203965"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="VFL84_07690"
     CDS             complement(<1..1125)
                     /locus_tag="VFL84_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET7608541.1"
                     /translation="MTRRFRQLGRMLTIQRVLMKHGLDEIVWRTHLFRPLAWLGRLLS
                     FGRRRRPLGVRLRTALEELGPIFVKFGQALSVRPDLLPPDIAAELAKLQDQVPPFAGE
                     QATAALTAAFGKPIEQVFASFERTPLAAASIAQVHAARLPDGRDVVVKILRPNVGALI
                     QRDVEVLYAIAGLAERYWPEARRLHPVEVVAEFERTLAVELDLMREAANASQLERNFA
                     GSALLHVPEVHWDYCRQGVLTIERIVGIRVDDLDALRAAGADIKRLAENGVEIFFTQV
                     FRHNFFHADMHPGNIFVDVTDPARPKYVAVDFGIVGTLDERDKHYLAENFLAFFRRDY
                     RRVATLHVDSAWVPAGTRVDELEAAVRAVCEPIFNKPLKDI"
     gene            complement(1122..1703)
                     /locus_tag="VFL84_07695"
     CDS             complement(1122..1703)
                     /locus_tag="VFL84_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET7608542.1"
                     /translation="MSFIEAELNRCIGESTAARDLLARLGGTSFAVHVEGLGLTGVLH
                     ADGERVRLDSDATAATATLSATPFDLLRLLRADGISGVKRTRAALSGDLDVAERYARM
                     LKLARPDLEEEVAKWLGDVPAHALGEVVRGFGAWLGRAGAALRMNTAEYLQEESRAMP
                     APLEAQAFYSDVERLRDDVERFAARLARAERSR"
     gene            complement(1700..2470)
                     /gene="ubiE"
                     /locus_tag="VFL84_07700"
     CDS             complement(1700..2470)
                     /gene="ubiE"
                     /locus_tag="VFL84_07700"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HET7608543.1"
                     /translation="MKANDPGERTERVADFGYEQVPWDEKKARVRGVFDSVASRYDLM
                     NDAMSGGLHRIWKRFTIAKTGLRPGQQALDVAAGSGDLAVGLARRVGASGRVVVTDIN
                     AAMLGEGRNRLLNAGVAGNVAYVQADAEQLPFAAGSFHCVTIGFGLRNVTDKERALAS
                     LYDVLKPGGRLLVLEFSHAHLGPLAPLYELYSFQVLPRLGALLAGDAARYRYLAESIR
                     RHPDQETLKGMMQRAGFERCEYFNLSAGIVALHLGFRL"
     gene            complement(2534..2890)
                     /locus_tag="VFL84_07705"
     CDS             complement(2534..2890)
                     /locus_tag="VFL84_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HET7608544.1"
                     /translation="MNTQIHVCRRLTAIVLTLILADPVAGLEPGQGFDAGRSKTVDGS
                     DLDVSRRADAEVLYARIRSAAHSVCRAEKAAWDGKAVSHQRQCVAEAVETAVARANEP
                     LLTAIHRANGERVAER"
     gene            3010..>3251
                     /locus_tag="VFL84_07710"
     CDS             3010..>3251
                     /locus_tag="VFL84_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7608545.1"
                     /translation="MDDSERQPTYLFEGFRLDTRRRTLVGPDGRAVALAPKTLDTLCY
                     LAARAGQIVGKRELLEAIWPHVIVEENNLNQVVSQLR"
BASE COUNT          525 a         1118 c         1113 g          495 t
ORIGIN      
        1 gatgtccttc agcggcttgt tgaagatcgg ctcgcagacc gcgcgcacgg cggcttcgag
       61 ctcgtcgacg cgcgtgccgg cgggcaccca ggccgagtcc acgtgcagcg tggcgacgcg
      121 ccggtagtcg cggcggaaga acgcgagaaa attctcggcg agatagtgct tgtcgcgctc
      181 gtcgagcgtg ccgacgatgc cgaagtcgac ggcgacgtac ttcggcctcg cgggatccgt
      241 gacgtcgacg aagatgttgc cggggtgcat gtcggcgtga aagaagttgt gccgaaacac
      301 ctgcgtgaag aagatctcga cgccgttttc ggcgagccgc ttgatgtccg cgccggccgc
      361 ccgtagcgcg tccaaatcgt cgacgcgaat gccgacgatg cgctcgatcg tgagcacgcc
      421 ttgccggcag taatcccagt gcacctccgg cacgtgcagc agcgccgagc ccgcgaagtt
      481 gcgctcgagc tgcgaggcat tcgcggcctc gcgcatgaga tcgagctcga ccgccagcgt
      541 acgctcgaac tccgcgacga cctcgacggg atgcagccgg cgcgcttcgg gccagtagcg
      601 ttccgcgagc ccggcgatcg cgtagagcac ctcgacgtcg cgctggatca acgcgccgac
      661 gttcggccgc agaatcttca cgacgacgtc gcgcccgtcc gggaggcgcg ccgcgtgcac
      721 ttgcgcgatc gaggccgccg cgagcggcgt gcgctcgaac gacgcgaaca cttgctcgat
      781 gggcttgccg aacgccgccg tgagcgccgc ggtcgcctgc tcgccggcga acggcggcac
      841 ttgatcctgc agcttcgcga gctctgcggc gatgtcgggc ggcaggagat cgggccgaac
      901 ggacagggcc tggccgaatt tcacgaagat cggcccgagc tcctcgagcg ccgtgcgcag
      961 ccgcaccccg agcggcctgc gcctgcggcc gaacgagagc aatcggccga gccatgcgag
     1021 cggccggaac aggtgcgtgc gccacacgat ctcgtcgagc ccgtgcttca tcagcacgcg
     1081 ctggatcgtg agcatgcggc cgagctgccg gaagcggcgc gtcatcggct tcgctcggcg
     1141 cgcgcgagcc gcgccgcgaa ccgctcgacg tcgtcgcgca gccgctcgac gtcggagtag
     1201 aacgcttgcg cttcgagcgg cgccggcatc gcgcggcttt cctcctgcag atactcggcc
     1261 gtgttcatgc gcagcgcggc acccgcgcgg ccgagccacg cgccgaagcc gcgcacgact
     1321 tcgccgagag cgtgcgccgg cacgtcgccg agccatttcg cgacttcctc ttcgagatcg
     1381 ggccgcgcga gcttcagcat gcgcgcgtat cgctcggcga cgtcgagatc gccggacagc
     1441 gccgcgcgcg tgcgcttgac gccgctgatg ccgtcggcgc gcagcaggcg cagcagatcg
     1501 aacggcgtcg ccgacagcgt cgccgtcgcg gccgtcgcgt ccgaatcgag ccgcacgcgc
     1561 tcgccgtcgg cgtgcaggac gcccgtgagc ccaagccctt cgacgtgcac ggcgaagctc
     1621 gtgccgccca agcgcgcgag cagatcacgc gcggccgtcg actcgccgat gcagcgattg
     1681 agctcggctt cgatgaagct caaaggcgga aacccaagtg gagggccact atccctgcgc
     1741 tcaagttgaa gtactcgcag cgctcgaatc ccgcgcgctg catcatgccc ttcaacgtct
     1801 cttggtcggg gtgacggcgg atcgactcgg cgagatagcg gtatctcgcc gcgtcccctg
     1861 cgagcaaagc cccaagccgc ggcaacacct gaaacgaata gagctcgtag agcggcgcca
     1921 ggggccctaa gtgcgcgtgc gagaactcga gcacgagcag ccggccgccg ggcttcaaga
     1981 cgtcgtagag actcgcgagc gcgcgctcct tgtccgtgac gttgcgcagg ccgaagccga
     2041 tcgtcacgca gtggaagctg ccggccgcaa acggcagctg ctcggcgtcg gcctgcacgt
     2101 aagcgacgtt gcccgcgacg ccggcgttca agaggcgatt gcggccctcg ccgagcatcg
     2161 cggcgttgat gtccgtgacg acgacgcggc cgcttgcgcc gacgcgccgc gcgaggccga
     2221 cggccagatc gccgctgccg gccgcgacgt cgagcgcctg ctggccgggc cgaagacccg
     2281 tcttcgcgat cgtgaagcgc ttccagatcc ggtgcagccc gcccgacatc gcgtcgttca
     2341 tgaggtcgta gcgcgatgcg accgagtcga agacgccgcg gacgcgggct ttcttctcgt
     2401 cccacggcac ctgctcgtag ccgaaatcgg cgactcgctc ggttcgttcg ccgggatcgt
     2461 tggccttcat cgagccgcag tataggccag gagccgcggt ccggcggcgc cgcagcgccg
     2521 ccggaaccgg cgtctaacgc tcggcgacgc gctcgccgtt cgcgcgatgg atcgccgtca
     2581 acaggggctc gttggcgcgg gcgacggcgg tctcgaccgc ctccgcgaca cactggcgct
     2641 ggtggctaac ggccttgccg tcccacgcag ccttctcggc gcgacacacc gaatgagcgg
     2701 cggagcggat acgtgcgtac agcacttctg catccgcccg gcgcgaaacg tcgagatcgg
     2761 agccgtccac cgtcttgctc cttccggcgt cgaaaccctg gccgggctcg agtccggcga
     2821 cggggtccgc gagaatcagg gtcaaaacga tggcggtcag gcggcgacaa acgtggattt
     2881 gcgtgttcat gaaatcgtcc tcctaggtga ttgggtgtct gaatcgggcc aggcgtagaa
     2941 ttcgcccgcg cggcctgagg actccttgtt caggcgctga agctttctga ggttgtctga
     3001 aggacgtccg tggacgactc cgaacgccag cccacgtacc tgttcgaggg gtttcgcctc
     3061 gatacgcggc gtcgaacgtt ggtcggcccc gacggccgag ccgtagcgtt ggcgccgaag
     3121 acgctcgaca cgctgtgcta cctcgccgca cgcgccgggc aaattgtcgg caaacgcgag
     3181 ctgctcgagg ccatctggcc gcacgtcatc gtcgaggaga acaacctcaa ccaagtggtc
     3241 tcgcagctgc g
//
LOCUS       DASSNN010000413         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1456762, whole genome shotgun sequence.
ACCESSION   DASSNN010000413 DASSNN010000000
VERSION     DASSNN010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456762"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFL84_07715"
     CDS             complement(<1..461)
                     /locus_tag="VFL84_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7608546.1"
                     /translation="MDERLAQASPILVIGMHRSGTRLVVDVLDKLGVFMGADRQGDAE
                     SVSLMMINEWLFHQCGAFWSEPMSVHFMLSQPEVVDELATSARRAFTAHLDKYVGPTR
                     WHQQGRAKPLPAFGWKDPRNTFTLPVWKQVFPSLRAIHIVRHGIDVAASLS"
     gene            complement(479..1879)
                     /locus_tag="VFL84_07720"
     CDS             complement(479..1879)
                     /locus_tag="VFL84_07720"
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HET7608547.1"
                     /translation="MTLVFPVPLVVEDIDAAARESIHAKVSRYLQSDRAKRDIEPSPE
                     ESVATSYYKHEAHVLADANLVELEHFVIATATTFLEKALKLPPRRLQIERSWINVFEP
                     GAQEAQHSHDGSLLSCSYYVEAPKDCGCIVMPDPIGARRSYREFTKTAGSDLLTRRDI
                     AVEPQAGRLVMFESWLPHYVQCNKSDRVRISIAMNLREAAAQVAAPRVAAAAAPAAPA
                     EDVLRAKSADAEPFLLNELFEVRPDLKLSLEPIQNTIPTVVVDDFLKHPERARDVVGR
                     APAANWKHVQGGKNFVDYYDCRLRFPIFYPNGMIAAAQQIIRQIYHVDTRPAEASVDV
                     NWFKQIREKRGESAVPHNDMTDKARRSFTCIVYLNRREECSGGTAFFRFKKTGSLVLD
                     EKYVDAIRENPKLAETGRDYWPHEADESWERVGTIDMVPGRLLIFPSEYFHAAYHPQN
                     SFFEFPRLTLAFWMVA"
     gene            complement(1895..>2194)
                     /locus_tag="VFL84_07725"
     CDS             complement(1895..>2194)
                     /locus_tag="VFL84_07725"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HET7608548.1"
                     /translation="HTHPGALWSATYYVDDGGVGSDPSLGGEFEIQDPRGVAPVMYAP
                     SLTFTGPDGAALGESQRLTPQAGMFVVFPSWLSHGVRPYRGTRERISIAINFSLG"
BASE COUNT          383 a          707 c          723 g          381 t
ORIGIN      
        1 tgcgagaggc tcgcggctac gtcgatgccg tggcgcacga tgtgaatggc gcgcaagctc
       61 ggaaatacct gcttccacac cggcagcgtg aacgtgttgc gcgggtcctt ccaaccgaag
      121 gccggcaatg gcttcgcgcg cccttgctgg tgccaacgcg tggggcccac gtacttgtcg
      181 aggtgcgcgg tgaatgctcg acgcgcgctc gtcgcgagct cgtcgacgac ctcgggctgc
      241 gacagcatga aatggaccga catcggctcg gaccagaacg ctccgcactg atggaagagc
      301 cactcgttga tcatcatcaa cgagacggac tcggcatcac cctgccgatc cgctcccatg
      361 aacacgccga gcttgtcgag cacgtcgacg acgagccgcg tgcccgagcg gtgcatgccg
      421 atcacgagga tggggctcgc ttgtgccaga cgttcgtcca tttcaaccct cactcccttc
      481 aggccaccat ccagaacgcg agcgtcaagc gcggaaactc gaagaacgag ttttgcgggt
      541 gatacgccgc atgaaagtac tcggacggaa agatcaagag gcgccccggc accatgtcga
      601 tcgtgccgac gcgctcccac gattcgtctg cttcgtgagg ccaatagtcg cgccccgttt
      661 ctgcgagctt gggattctcc cgaatcgcgt cgacgtattt ctcgtcgagc acgagcgagc
      721 ccgttttctt gaaacggaag aacgccgtgc cgcccgagca ttcttcgcgc cgattcaagt
      781 agacgatgca cgtgaacgac cggcgcgcct tgtccgtcat gtcgttgtgc gggacggcgg
      841 actcgccgcg tttctcgcgg atctgcttga accaattgac gtcgacgctc gcctcggccg
      901 ggcgcgtgtc gacgtgatag atctgccgaa tgatctgctg cgctgcggca atcatgccgt
      961 tcggatagaa gatcggaaac cgcagccggc aatcgtagta atcgacgaag ttcttgccgc
     1021 cttgcacgtg cttccagttc gcggccggcg ccctgccgac gacgtctctc gctcgctcgg
     1081 gatgcttgag aaagtcgtcg acgacgacgg tcgggatcgt gttttgaatc ggctccaagc
     1141 tcagcttcag gtcggggcga acctcgaaca gctcgttcag caggaacggc tccgcgtcgg
     1201 cgctcttcgc gcggaggacg tcctcggcgg gggccgccgg cgccgcggcc gccgccacac
     1261 gcggcgcagc gacctgcgcc gcggcctcgc gcaagttcat cgcgatcgag atcctgaccc
     1321 ggtcgctctt gttgcactgg acgtagtggg gcagccacga ctcgaacatg acgagccggc
     1381 ccgcctgcgg ctcgacggcg atgtcgcgcc gcgtaagcaa atcgctgccg gccgtcttcg
     1441 tgaattcccg gtagctgcgc cgcgcgccga tcgggtcggg catcacgatg cagccgcagt
     1501 ccttgggcgc ttcgacgtag taactgcacg aaagcaggct gccgtcgtgc gaatgctgcg
     1561 cctcttgcgc gcctggctcg aagacgttga tccaggagcg ttcgatctgg agccggcgcg
     1621 gcggcagctt cagcgccttc tcgagaaagg tcgtcgccgt cgcgatgacg aagtgctcga
     1681 gctcgaccaa gttggcgtcg gccagcacgt gcgcctcgtg cttgtaataa gaagtcgcga
     1741 ccgactcctc gggcgacggc tcgatgtcgc gcttggctcg gtcggactga aggtaacggg
     1801 acaccttcgc gtgaatggac tcgcgcgctg ccgcgtcgat gtcctcgacg acgagcggca
     1861 cgggaaagac gagcgtcatc gacacgggtg cacgctaacc aaggctgaag ttgatcgcga
     1921 tcgagatgcg ctcgcgcgtg ccgcgatacg gacgcacgcc gtgcgagagc cagctcggga
     1981 agacgacgaa catgccggcc tgcggggtca gccgctgcga ctcgccgagc gcggcgccgt
     2041 cggggcccgt gaacgtcagc gacggcgcgt acatgacggg cgcgacgccg cgcgggtcct
     2101 ggatctcgaa ctcaccgccg agcgaggggt cggagccgac gccgccgtcg tcgacgtagt
     2161 acgtcgcgga ccagagcgcg ccgggatgcg tgtg
//
LOCUS       DASSNN010000414         4034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1305498, whole genome shotgun sequence.
ACCESSION   DASSNN010000414 DASSNN010000000
VERSION     DASSNN010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305498"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFL84_07730"
     CDS             <1..215
                     /locus_tag="VFL84_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608549.1"
                     /translation="HKDKGMKELLEPRGPEVYLYFPDGMGRSKLTTAVIEKYLDSPGT
                     ARNWNTLTKLVEVGRELEAKEPRSRS"
     gene            complement(212..1150)
                     /locus_tag="VFL84_07735"
     CDS             complement(212..1150)
                     /locus_tag="VFL84_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608550.1"
                     /translation="MGVRDRLRGLGKRRGVRFAASLPERVVRSASALAAGTAREVAAI
                     ALPSGFRRTRLYRNLVDVTLQFLVEDVGGVQSAQPTEQKLGEDFLLRRAAGNGIELMG
                     VVAFRASPVWVLAALADVAGFGRQLIPEIAESLKKEGLLAPEDSFSTVEQLLGGLERS
                     SAQLADAVNAPPLDVAGLRNEWRKFAAEARQLPAPQLPSAASVTRLWQDLRATAERER
                     RSPFEVSSLLAVAAVGELPERARVLSKSAAVVLRHGGAAVSNALLEHYRQSLRELKDV
                     GYVRYGALKLTPYTRAAIEAFHPSRETLTGKLLDKL"
     gene            complement(1397..2200)
                     /locus_tag="VFL84_07740"
     CDS             complement(1397..2200)
                     /locus_tag="VFL84_07740"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET7608551.1"
                     /translation="MDSLQIVVLAVVQGITEFLPISSSGHLILIPFFADWPDQGLEFD
                     VAVHIGTLAAIVVYFRKTLAAMARDFALSVVQRREVGESRLAWAVLFGTIPAGVVGLL
                     FRHDIETTLRSPWVVASTTVFYALLLLVADRRRGARDEHSLGWVDVVVIGCAQALSLV
                     PGTSRSGVTMTAGLFRNLSREAAARFSFLLAVPVMTATGLADLLGYTGEQAAGPADTR
                     AIVLGLVLSAATGFACIHYFLKWLTRFGMLPYVIYRLALGTILFIVLSR"
     gene            2415..2627
                     /gene="tatA"
                     /locus_tag="VFL84_07745"
     CDS             2415..2627
                     /gene="tatA"
                     /locus_tag="VFL84_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET7608552.1"
                     /translation="MGLAMSPFQLLIILLIVALIFGTKRLKDIGGDLGSAVKSFRKAM
                     NTSGEIDDAPADPPKQLSRDEEKQKQ"
     gene            2682..3629
                     /gene="secF"
                     /locus_tag="VFL84_07750"
     CDS             2682..3629
                     /gene="secF"
                     /locus_tag="VFL84_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET7608553.1"
                     /translation="MQWLTRTPNFNFVKWRRVAMATSTLLSLLAIVAIFYPGLNYGLD
                     FTGGVLVEVRYPSTPQVDEIRMELTEAGLTSAQVTNSGSDVLIRLPPPEGEDGAELGA
                     EAVAAVRDRVLEILRMHEPNVEFRSAANISGQVGEELFENAGLALLFALIMIFIYVML
                     RFRWKLAVGAIVATVHDVLVTTGIFAIAGWQFDLTVLGSILAVLGYSINDTIVVYDRI
                     RDNFRLMRRGTPEAIVNASVNQTLARTLVTGVTTLLVLFAMIFLAGDTLWGFSVALVI
                     GILVGTYSSIYVASSMALALKVEASDFVEAKKVHADELP"
     gene            3638..>4034
                     /locus_tag="VFL84_07755"
     CDS             3638..>4034
                     /locus_tag="VFL84_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7608554.1"
                     /translation="MSREAPRVRLERPSARRERDYLQAAQRSRALHRGFVVPASAPRE
                     YRAYLRAARRVNQESFFVLDAQSGELAGVININDIVRQAQLSGRLGYYAFVPHAGRGL
                     MREGLSLVIARAFRELGLHRLEANIQPGNR"
BASE COUNT          735 a         1323 c         1318 g          658 t
ORIGIN      
        1 ggcacaagga caagggcatg aaagagctgc tcgagccccg cggccccgag gtatatctct
       61 acttccccga cggaatggga cgctcgaaac tcacgacggc agtcatcgag aagtacctcg
      121 attcgccggg cacggcacgc aactggaaca cgctgaccaa gctagtcgag gtcggccgcg
      181 agctcgaggc caaagagccg aggtcgcgga gctagagctt gtcgagcagc ttgcccgtca
      241 acgtctcacg cgacggatgg aacgcctcga tggcggcgcg cgtgtacggc gtgagcttca
      301 gcgcaccata gcggacgtag ccgacgtctt tgagctcccg cagcgactga cggtagtgct
      361 cgagcagcgc gttcgaaacg gccgcgccgc cgtggcgtag cacgaccgcg gccgacttcg
      421 acagcacgcg cgcccgttcc ggcagctcgc cgaccgcggc gacggcgagc agcgacgaca
      481 cctcgaacgg cgagcgccgc tcgcgctcgg ccgttgcgcg cagatcctgc cacagccgcg
      541 tcacgctcgc agccgagggc aattgcggag ccggcaattg ccgagcctcg gcagcgaact
      601 tgcgccactc gttgcgcaag cccgcgacgt cgagcggcgg ggcgttgacg gcgtcggcga
      661 gctgagcgga gctgcgctcg aggccgccga gaagctgctc gaccgtcgag aacgaatcct
      721 cgggcgccag caagccttcc ttcttcaagc tctcggcgat ctccggaatc agctgccggc
      781 cgaagccggc gacgtccgcg agcgcggcga gcacccacac cggcgacgca cgaaacgcga
      841 cgacacccat gagctcgatg ccgttgcccg cggcgcggcg cagcaagaag tcctcgccga
      901 gcttttgctc ggtcggctgc gcgctctgca cgccgccgac atcctcgacg aggaattgca
      961 gcgtgacgtc gacgagattg cgatagagcc gcgtgcggcg aaaaccgctc ggcagcgcga
     1021 tcgcggcgac ttcgcgtgcc gtgcccgcgg ctagcgcgga cgccgagcga acgacgcgct
     1081 cgggcagcga cgcggcgaag cgcacgccgc ggcgcttgcc gaggccgcgg agcctgtctc
     1141 tgacacccac ggcgtgaacc gtatcacgat cgagcgcgac cgtgccgcag cgctcttgcg
     1201 ttttgggcgg cgaataggcg aatcttacgg ccaggtttaa caacccaaag gaggtgatcc
     1261 catgtctagt gagattcttg gggtttcgat gcggtgtgag cgttcctgcc aagggcgagg
     1321 cgcttagcaa cgcgtacctt ttttgggttc agagaccccg tctcacggag ggccgcgcaa
     1381 gcggccctct ttgcattcag cgcgagagca cgatgaacag aatcgtgccg agggccagcc
     1441 ggtagatcac gtacggcaac attccaaaac gggtcaacca cttcaagaag tagtgaatgc
     1501 aggcgaaacc cgttgcggcc gacagcacga gcccgagcac gatggctcgc gtgtccgcgg
     1561 gtcccgcggc ctgctcacct gtgtatccca agagatcggc gagcccggtc gcggtcatga
     1621 cggggacggc gagcagaaac gagaagcgcg ccgccgcctc gcgcgataga ttgcgaaata
     1681 gaccagcggt catcgtcacg cccgagcgcg aggtcccggg cacgagcgag agcgcttgcg
     1741 cgcagccgat cacgacgacg tcgacccagc cgagcgagtg ctcgtcgcgc gcgcctcgcc
     1801 ttcgatccgc gacgagcaaa agcaacgcgt agaagacagt cgtggacgcc acgacccacg
     1861 gcgagcgcag cgtcgtctcg atgtcgtggc ggaacagcag ccccacgacg ccggcaggta
     1921 tcgtgccgaa caggacggcc cacgcgagcc ggctctcgcc gacttcgcgc cgctgcacga
     1981 cggagagcgc gaagtcgcgc gccatcgcgg cgagcgtctt gcgaaagtac acgacgatcg
     2041 ccgccagcgt gccgatgtgg acggcgacat cgaactcgag gccttggtcg ggccaatcgg
     2101 cgaagaaggg gatcaggatc aggtgaccgg agctcgagat cggcaagaat tccgtgatcc
     2161 cttgaacgac ggcgagaacg acgatctgca aagaatccat taggccgacc aggtcgttgt
     2221 gcgtaccggc gcgtatcgta gccgacgaat gcgagccgcc ggtcacgcgt cgtgaactgg
     2281 cgcccctctg gcgtggtcga tatggcgcgg gcggggcata actgcagtgg gcgcccgaat
     2341 tggcttttct gtgctccccc ctgcaaaatg acgtttcgac gcgctatcca gcggccgtcg
     2401 aatggagaca atttatgggt ctggcgatga gcccttttca gctgctgatc atcctgttga
     2461 tcgtggcgct gatctttggc acgaagcgtt tgaaggacat cggcggcgac ctgggcagcg
     2521 ccgtcaagag ctttcgtaag gccatgaaca cctctggcga gatcgacgac gcgccagccg
     2581 atcctccgaa gcagctctcg cgcgacgaag aaaagcaaaa gcagtagtct tccctccttc
     2641 cgctatccgc cgggaacggt tgggccgttc ctcttatccg tatgcagtgg ctgacacgca
     2701 cgccgaattt caacttcgtc aaatggcgcc gcgtcgcgat ggcaacgtcg acgctgctct
     2761 cgctgcttgc gatcgtcgcc atcttctacc ccggcttgaa ctacggcctc gacttcacgg
     2821 gcggtgttct cgtcgaagtc cgatatccct cgactccgca ggtcgatgag atccggatgg
     2881 agctgaccga ggccgggcta acgtccgcgc aggtgacgaa ctcgggcagc gacgtgctga
     2941 ttcgcttgcc gcctcccgaa ggcgaagacg gcgcagagct cggcgccgaa gccgtcgccg
     3001 ccgtccgcga tcgtgttctc gagatattgc gaatgcacga gccgaacgtc gaatttcgca
     3061 gtgcggcgaa catcagcggc caagtcggcg aggagctgtt cgagaacgcc ggtctcgcgc
     3121 tgctgttcgc gctgatcatg atcttcatct acgtgatgct gcgcttccgc tggaagctcg
     3181 cggtaggcgc gatcgtcgcg acggtccacg acgtactcgt cacgacgggc atcttcgcga
     3241 tagcgggctg gcaattcgat ctcacggtgc tcggctcgat cctcgccgtg ctcggctatt
     3301 cgatcaacga cacgatcgtc gtctacgacc gcattcgcga caacttccgc ttgatgcgcc
     3361 gcggcacgcc ggaggcgatc gtcaacgcgt ccgtgaacca gacgctcgcg cgcacattgg
     3421 tcaccggcgt gacgacgttg ctcgtgctgt tcgcgatgat cttcctcgcc ggcgacacgc
     3481 tctggggctt ctccgtggcg ctcgtgatcg gcatcctcgt cggcacttac tcgtcgatct
     3541 acgtcgcgag ctcgatggcg ttggcgctga aagtcgaggc gtccgacttc gtcgaggcca
     3601 agaaggtcca cgcggacgag ctgccgtagc gtagcgcgtg agtagggaag cgccgcgcgt
     3661 ccgcctcgaa cgcccgagcg cgcgccgcga gcgcgactat ttgcaggccg cgcagaggag
     3721 cagagcgctg catcggggct tcgtcgtccc ggcgtcggcg ccgcgcgagt atcgtgccta
     3781 cctgcgcgcc gcgcgccgcg tcaatcaaga gtcgttcttc gtcctcgacg cgcaatcggg
     3841 cgagctcgcc ggcgtcatca acatcaacga catcgtgcgg caggcgcagc tgtcgggccg
     3901 gctcggctac tacgcgttcg ttccgcatgc cgggcgcggg ctcatgcgcg aggggctctc
     3961 gctcgtcatc gcgcgcgcat tccgtgagct cggcctgcac cggctcgaag cgaacatcca
     4021 acccggcaat cgcc
//
LOCUS       DASSNN010000415         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_936294, whole genome shotgun sequence.
ACCESSION   DASSNN010000415 DASSNN010000000
VERSION     DASSNN010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936294"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1934
                     /locus_tag="VFL84_07760"
     CDS             <1..1934
                     /locus_tag="VFL84_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7608555.1"
                     /translation="AGGVTLPAYLPGGAYGLNCPPTGGCTNSQAFPVPTELAFLLDSR
                     AQPNNNFQLQRYLNFMPSRATNNRSNVYQLQTGVEGSLKDGDWTWEAYISHGETTVDS
                     YLYEGWASDQRYKQVAQAPNFGRGFTQTSATGALGFNIRCTTGLPLVAEFTPSDDCID
                     AIDARMKQFTRLEQTIVEANMQGGIVEMRSGELRFAAGLSSRENTALFEPDPLVDAQS
                     TRDNPIGLFPQNETSGETDVSEIYGELLIPVLDRLDFELGYRHSDYDQEGGIDTYKAL
                     FDWAATDSFRVRGGRQIANRAPNVAERFTGPSQSVVAFQGADPCLANTLNTWGNHPSN
                     PNRAQVQALCSAIINAPPGQPSSWDQAPNTYTGPFPFVFQLEVEERRGNADVRSEEAE
                     TYTLGAVFQRERWSASLDFYTIDIKDAIAPPDAYFVYNRCFNGAGTNPSYSIDDPEGF
                     CRLIVRDPISGYRVQVNTLYRNLGALETDGVDLQLNWNGNIGSNGVFVNFLASFVDSY
                     KLQQQPGGAFVEYAGTLGAGGQYDYRTFTTFGYDFGDSVNLGLRWTHLPEVENAVFAT
                     NPNTTVLPTSAYDRFDLFGRWSPRETIELRFGIDNLADADPEIVGANPGQTNAMGLTN
                     TGFYDILGRRYYAGVNFSF"
     gene            complement(1931..2407)
                     /locus_tag="VFL84_07765"
     CDS             complement(1931..2407)
                     /locus_tag="VFL84_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HET7608556.1"
                     /translation="MYYFLLQYVHIIGAAVLLGTGAGIAFFMLRAHSTRDVFVIAGVA
                     RIVVAADFVFTATAVVVQPVTGFLLAKFLGYPIFDGWIALSLILYAVTGAFWIPVVFM
                     QIRMRNLAEAAVREGTALPAAYHRLFWTWFAFGFPAFAAVLGILWLMTSRPMFSFF"
     gene            complement(2419..>2604)
                     /locus_tag="VFL84_07770"
     CDS             complement(2419..>2604)
                     /locus_tag="VFL84_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX-like family protein"
                     /protein_id="HET7608557.1"
                     /translation="GIAFRRTARGALWAAIGLSIFYMIAGTLVLPALWLDPVGPMLKI
                     WPIIALNLVALAVVEDR"
BASE COUNT          513 a          865 c          819 g          407 t
ORIGIN      
        1 gcgccggcgg cgtaactctt ccggcgtact tgccgggcgg cgcgtatggc ctgaattgcc
       61 ctcctacggg cggctgcacg aactcgcagg cgttcccggt gcccacggag cttgcgttct
      121 tgctcgattc gcgcgcccaa ccgaacaaca actttcagct gcagcgctac ctgaatttca
      181 tgccgtcgcg tgcgaccaat aatcgcagca acgtgtacca gctgcagacc ggcgtcgagg
      241 gctcgctcaa ggacggcgac tggacgtggg aagcgtacat ctcgcacggc gagaccacgg
      301 tcgacagcta tctatacgaa ggctgggcgt cggaccagcg ctacaagcaa gtcgcgcagg
      361 cgccgaactt cggccgcggc ttcacgcaaa cgtcggcgac cggagcgctc ggtttcaaca
      421 tccgctgcac gaccgggctg ccgctcgtcg cggagttcac gccgtccgac gactgcatcg
      481 acgcaatcga cgcacgcatg aagcagttca cgcggctcga gcagacgatc gtcgaggcca
      541 acatgcaggg cggcatcgtc gagatgcggt cgggcgagct gcggttcgcc gccggcttga
      601 gcagccgcga gaacacggcg ctgttcgagc cggatccgct cgtcgacgcg cagtccacgc
      661 gcgacaatcc gatcggcctg tttccgcaga acgagacttc gggcgagacc gacgtatcgg
      721 agatttacgg cgagctcctg atcccggtct tggaccgact cgatttcgag ctcggctacc
      781 gccattccga ctacgaccag gaaggcggca tcgacactta caaggcgctg ttcgactggg
      841 ctgcgacgga ctccttccga gtgcgaggcg ggcggcagat tgcgaaccgc gcgccgaacg
      901 tcgcggagcg cttcacgggg ccgtcgcaga gcgtcgtcgc gtttcagggc gccgatccgt
      961 gcctcgcgaa cacgctcaac acctggggca accatccgag caacccgaat cgggcgcagg
     1021 tccaggcgct ctgctcggcc atcatcaacg cgccgcccgg ccagccttct tcgtgggatc
     1081 aggccccgaa cacgtacacg gggccgttcc cgttcgtgtt ccagctcgag gtcgaggagc
     1141 ggcgcggcaa cgccgacgtg cgatcggagg aagccgagac ttacacgctc ggcgccgtgt
     1201 tccagcgcga gcgttggtct gcctcgctcg atttttacac gatcgacatc aaggacgcga
     1261 tcgcgccgcc cgacgcctat ttcgtctata accgctgctt caacggcgcg ggaacgaatc
     1321 cgagctactc gatcgacgat cccgaaggat tttgccggct catcgtccgc gatccgatct
     1381 cgggctaccg agtgcaagtc aatacgctct atcgaaacct cggcgcgctc gagaccgacg
     1441 gcgtcgatct gcagctcaat tggaacggca acatcggctc gaacggcgtc ttcgtcaact
     1501 tcctcgcgag cttcgtcgac agctacaagc tgcagcaaca gccgggcggc gctttcgtcg
     1561 agtacgcggg cacgctcggc gccggcggac agtacgacta tcggacgttc acgaccttcg
     1621 gctacgactt cggcgacagc gtcaatctcg gtttgcgttg gacgcatctg cccgaggtcg
     1681 agaacgccgt gttcgcgacg aacccgaaca cgaccgtgct gccgacgagc gcctacgacc
     1741 ggttcgacct attcggccgc tggtcgccgc gcgagacgat cgagctcaga ttcggtatcg
     1801 acaacctggc cgacgcggac ccggagatcg tcggtgcgaa tccgggccag acgaacgcga
     1861 tgggcctgac gaacacgggc ttctacgaca tcctcggccg tcgttactat gccggcgtga
     1921 acttcagctt ctaaaagaac gagaacatcg gccggctcgt catcagccac aagatgccga
     1981 gcacggccgc gaacgccggg aagccgaacg cgaaccaggt ccagaagagc cgatggtacg
     2041 cggccggtag cgccgttcct tcgcgaaccg cggcctcggc cagattccgc atgcggatct
     2101 gcatgaacac gacggggatc cagaacgcgc cggtcaccgc gtacaggatc agcgacagcg
     2161 cgatccagcc gtcgaagatc gggtagccga gaaacttcgc gagcaagaag cccgtcacgg
     2221 gctgcacgac gacggccgtc gccgtgaaca cgaagtccgc cgcgacgacg atgcgcgcga
     2281 cgccggcaat gacgaacacg tcgcgcgtcg agtgcgcccg cagcatgaag aacgcgatgc
     2341 cggcaccggt gccgagcagc acggccgcgc cgatgatgtg cacgtactgc agcaggaagt
     2401 agtacagcgt catgcgactc agcgatcttc gacgacggcg agcgcgacca agttcagcgc
     2461 gatgatcggc cagatcttca gcatcggccc gaccgggtcg agccacaatg cgggcagcac
     2521 caacgtgccg gcgatcatgt agaagatcga taggccgatt gcggcccata acgcgccgcg
     2581 cgccgtgcgc cgaaaggcaa tgcc
//
LOCUS       DASSNN010000416         3437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_451332, whole genome shotgun sequence.
ACCESSION   DASSNN010000416 DASSNN010000000
VERSION     DASSNN010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451332"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..852)
                     /gene="clpP"
                     /locus_tag="VFL84_07775"
     CDS             complement(229..852)
                     /gene="clpP"
                     /locus_tag="VFL84_07775"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HET7608558.1"
                     /translation="MDTRALNLVPIVVEQTARGERSYDIYSRLLKDRVVFVVGPIDDY
                     MANVVVAQLLFLESENPDKDVHLYINSPGGIVTAGLSIYDTMQFIKPDVSTICLGQAA
                     SMGSLLLTGGAKGKRYCLPHSRIMIHQPSGGFQGQASDIDIHAREVLETRDRLNRILS
                     KHTGQTIEQIRHDTDRDNFMGGEAAVAYGLIDRVMERRFDAAAASAK"
     gene            complement(877..2196)
                     /gene="tig"
                     /locus_tag="VFL84_07780"
     CDS             complement(877..2196)
                     /gene="tig"
                     /locus_tag="VFL84_07780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET7608559.1"
                     /translation="MNATNSKSYEVSLETSAGLERRLTVRVPNAEIERAIAARLAQVG
                     KTAKLKGFRPGKVPQKVVRQYYGGQVRDEVMTDVIRTTYSRAIEEKKLNPAGGPRIEP
                     LAAGDAGAAEHFTYRATFEVYPEFTLKDLAELKIDVPSVDVEEPDLDAMIEKLREQRA
                     TWAVVERGGEEGDRVTVDFTGTIGGEPFEGGQGKGIGIVIGAGQVLKDFDAALRGVKA
                     GESTTATVEFPEDYPTQNLAGKTAQFAIDVKKVEERRLPELDDTFAESFGLAGGAASL
                     RGEVRNNMQRELKERLRAETKTRAFDALIAANRIVVPRALVDQEISALQADALRQMGS
                     SDPKDAPPRERFEAAAQRRVTIGLLIQELLREHKIKLEQPRVEQRIKELAAPYEKPDE
                     AAQFYRSDRGMMAQVEASVLEDQVVDFLLSRASCTTKKLSFKDFMGA"
     gene            complement(2222..2306)
                     /locus_tag="VFL84_07785"
     tRNA            complement(2222..2306)
                     /locus_tag="VFL84_07785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2270..2272),aa:Leu,seq:tag)
     gene            complement(2487..>3437)
                     /locus_tag="VFL84_07790"
     CDS             complement(2487..>3437)
                     /locus_tag="VFL84_07790"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HET7608560.1"
                     /translation="RLQGTPTAANVGTFANIGIGVNDGQGGSAQLAAFSIVVSAPPNQ
                     PPSISGSPPTTVTAGTAYSFTPTASDPDGGTLTFSIANKPSWATFSATTGRLQGTPTS
                     ANVGTFSGIGIGVSDGQGGSAQLATFQIVVSAPPNRPPTITGTPPASVNVGMAYSFTP
                     TASDPDGDTLTFGVANLPAWASFDTATGRLSGTPAALNVGTTSGIVITVDDGRQGTAS
                     LAAFSVTVQAVSLGTATLTWLPPTTNTDGSPLTNLAGYKIYWGTSQGNYPNSTTLTNP
                     GLTSYVITNLAPGTYFFVATAYNSAGVESTFSGVASKTIN"
BASE COUNT          542 a         1137 c         1101 g          657 t
ORIGIN      
        1 ttcatgctgg cttttgccgc agaaggagca gtacagaagc ttgccgtcgt cgtgcttgcc
       61 gcgggaatcg tcggacatga atgaccctca atgaatcggc gcagacatcg ctacgatgag
      121 gaaacgatgt cccgggctat atatcacgcg tcgccattag gctgcaaaga cgccctgccc
      181 ggctgtgtcc ttgcgcgaaa aagtcagcaa aaaacctatt cgacttaatc acttagcgct
      241 tgctgcagca gcatcaaaac gacgctccat aacgcgatcg atgaggccgt acgcgaccgc
      301 agcctcgccg cccataaagt tgtcgcgatc cgtatcgtgg cgaatctgct cgatcgtctg
      361 accggtgtgt ttagacagaa ttcggttcag gcgatcgcgc gtctcgagca cttcgcgcgc
      421 atgaatatcg atgtcggagg cctgcccttg gaagccgccg ctcggctggt gaatcatgat
      481 gcgcgagtgc ggcaagcagt agcgcttgcc tttcgcgccg cccgtcagca acaacgatcc
      541 catgctcgcc gcttggccga gacagatcgt gctgacgtcg ggcttgatga attgcatcgt
      601 gtcgtagatc gacaagccgg ccgtcacgat gccgcccggc gagttgatgt aaagatgcac
      661 gtccttgtcc ggattctccg actcgaggaa caggagctgc gcgacgacga cgttcgccat
      721 gtagtcgtcg atgggcccga cgacgaagac gacgcgatcc ttgagcagcc gcgaataaat
      781 gtcgtacgac cgttcgccgc gcgccgtctg ctcgacgacg atcgggacca aattcaacgc
      841 tctcgtatcc attgttagct cctcgggtcg ccgcggttac gcacccatga agtccttgaa
      901 gctcagcttc ttcgtcgtgc agctcgcacg cgacagcaga aagtcgacga cctggtcttc
      961 gaggaccgag gcctcgacct gggccatcat gccacgatcg ctgcggtaga attgcgcggc
     1021 ctcgtccggt ttctcgtacg gcgccgcgag ctctttgatt cgttgctcga cacgcggttg
     1081 ctcgagtttg atcttgtgct cgcgcaaaag ttcttggatc aatagcccga tcgtcacgcg
     1141 ccgttgcgcc gcggcctcga agcgctcgcg aggcggcgca tctttcgggt cgctgctgcc
     1201 catctggcgc aacgcgtccg cttgcagcgc gctgatttcc tggtcgacga gcgcgcgcgg
     1261 caccacgatg cggttggctg cgatcaacgc gtcgaacgcg cgcgtcttcg tctcggcgcg
     1321 cagccgctcc ttgagctcgc gctgcatgtt gttgcgtacc tcgccgcgca gcgaggccgc
     1381 tccgccggcg agcccgaaac tttccgcgaa cgtgtcgtcg agctccggca agcgccgctc
     1441 ttcgactttc ttgacgtcga tcgcgaactg cgccgtcttg ccggcgagat tctgcgtcgg
     1501 gtagtcctca gggaactcga ccgtcgccgt cgtgctctcg ccggctttga cgccgcgcag
     1561 cgccgcgtcg aaatccttga gcacttggcc ggcgccgatc acgatgccga tgcctttccc
     1621 ctgaccgccc tcgaacggct cgccgccgat cgtgccggtg aaatcgacgg tgacgcggtc
     1681 gccttcctcg ccgccgcgct cgacgacggc ccaggtcgcc cgctgctcgc ggagcttctc
     1741 gatcatcgcg tcgagatcgg gctcctcgac gtcgacgctc ggcacgtcga tcttaagctc
     1801 ggccaggtcc ttcagcgtga actcggggta cacctcgaac gtcgcgcggt aggtgaagtg
     1861 ctcggccgcg ccggcgtcgc cggccgcgag cggctcgatg cgcggcccgc ccgccggatt
     1921 cagttttttc tcctcgatcg cacgcgaata ggtcgtgcga atgacgtccg tcatgacctc
     1981 gtcgcggacc tgcccgccgt agtactggcg gacgaccttc tgcggcacct tgccgggccg
     2041 aaaccccttg agtttcgcgg tcttacccac ttgcgcgagc cgtgccgcaa tggcccgctc
     2101 gatctcggcg ttcggcacgc ggaccgtcaa ccgccgttcc aacccggccg aggtctcgag
     2161 cgagacctcg taactctttg aattcgttgc gttcattgtc tggaccatgg atacgaggac
     2221 ttggtgcgaa aggggagact cgaactccca cgggttgccc cactggattc taagtccagc
     2281 gcgtctacca attccgccac tctcgcttcg gcgggataca cagggcggca ttatagacac
     2341 gccgggtcgg cgaatctcca agcggggcgg ccgcggcagc ggcgccgaat cgaggcgcgg
     2401 gccccgccac gcgggcgcgc ggcgggggtg gcgggagggc gctcgagcgc cccgagactc
     2461 gcccggggct gcggcgactc tttattttag ttgatggtct tgctggcgac gccggagaac
     2521 gtgctctcga cgcccgcgct gttgtaagcc gtggcgacga agaaatacgt gcccggcgcg
     2581 agattcgtga tcacgtagct cgtcaatccg gggttcgtca gcgtcgtcga gttcggatag
     2641 ttgccctgcg acgtgcccca gtagatcttg tagcccgcga gattcgtcag cggcgagccg
     2701 tccgtgttcg tcgtcggcgg cagccaggtc aacgtcgccg tgccgagcga gaccgcctgc
     2761 accgtcacgc tgaacgccgc gagcgacgcc gtgccctgac gaccgtcgtc gacggtgatg
     2821 acgattccgc tcgtcgtgcc gacgttcagt gcggccggcg ttccgctcaa ccggcccgtg
     2881 gccgtgtcga acgacgccca cgccggcagg ttcgcgacgc cgaacgtcaa cgtatcgccg
     2941 tcgggatcgc tggcggtcgg cgtgaacgag taagccatcc cgacgttgac ggacgccggc
     3001 ggcgtgcccg tgatcgtcgg cggccgattc ggcggtgcgc tgacgacgat ctggaacgtc
     3061 gcaagctggg ccgatccgcc ctggccgtcg ctgacgccga tgccgatgcc cgagaatgtg
     3121 ccgacgttcg cgctcgtcgg cgtgccctgc aaccggcccg tcgtcgcgct gaacgtggcc
     3181 cagctcggct tgttcgcgat gctgaacgtc agcgtgccgc cgtcggggtc gcttgccgtc
     3241 ggcgtgaacg agtaagccgt gccggccgtg accgtcgtcg gcggcgagcc cgagatcgac
     3301 ggcggctggt tcggcggcgc gctgacgacg atcgagaacg ccgcgagctg cgcgctgccg
     3361 ccctgcccgt cgttgacgcc gatgccgatg ttcgcgaacg tgccgacgtt ggcggcggtc
     3421 ggcgtgcctt gcagccg
//
LOCUS       DASSNN010000417         3700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_451396, whole genome shotgun sequence.
ACCESSION   DASSNN010000417 DASSNN010000000
VERSION     DASSNN010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451396"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFL84_07795"
     CDS             complement(<1..858)
                     /locus_tag="VFL84_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7608561.1"
                     /translation="MRGKQQPKQAKKKSAKEAASRQPRPPFPKQKQKPPGLESKLEPK
                     PRFEAPEYKAAGKLEGKVALITGGDSGIGRAVATLFAREGATVAINYLDEEQSDAEET
                     QTAVEEAGAECLLLPGDLSEPDTCEDLIDKTIEHFGGIDVLVSNAAYQRRKESLDELS
                     DEELQHTFETNIFAYMRLSRAALRHMKPGSAIIATSSETGILGSEKLPDYSATKGAIN
                     AFTKTLAQSVVKQGIRVNAVAPGPVWTPLIVMSGEAKDDPITFGADSPMRRPAQPAEL
                     APAYVFLASP"
     gene            complement(924..1697)
                     /gene="xth"
                     /locus_tag="VFL84_07800"
     CDS             complement(924..1697)
                     /gene="xth"
                     /locus_tag="VFL84_07800"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET7608562.1"
                     /translation="MRIATFNVNGVNARLAALTRWLEESKPDVACLQELKMPSERFPA
                     DALRAAGYEALWHGQKSWNGVAILSRGIAPVERRRVLPGDPEDLHSRYIEAAVGDIVV
                     GCLYLPNGNPAPGPRFDYKLRWLERLIRHGAELLAESKPVVLAGDFNVIPTDLDVYAP
                     ERWRDDALFRPEVRSAYETLLAQGWVDAVRALHPDERVYTFWKYFRNAFPRDAGLRID
                     HLLLSPPLAPRLLAAGVDREVRGREKASDHAPVWIEIRD"
     gene            1788..2678
                     /locus_tag="VFL84_07805"
     CDS             1788..2678
                     /locus_tag="VFL84_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET7608563.1"
                     /translation="MRRLGIWPLIKASIASWRKDHASSMGGALAYYTLFSIAPVLIIV
                     IAVAGFFLGPEAARGEIVAQLQGLLGPDGAAAVQALLESASKPEEGLLATITSVLLLL
                     LGATTIFAELQSDLDRIWRAETKPISGLWAFLRARILSFGMILVLAFLLLVSLVVSAA
                     LSALGKWWGGWFEGWAVLLEVLNFAVSFAITTGLFALIYKLLPRAEIAWHDVWIGAAV
                     TALLFAVGKFLIGLYIGRSGVASGFGAAGSFVVLLVWVYYSTQIFLLGAEFTWVYAHA
                     RGSRRGRDRPGARERATAAA"
     gene            2817..3302
                     /locus_tag="VFL84_07810"
     CDS             2817..3302
                     /locus_tag="VFL84_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7608564.1"
                     /translation="MRRAITAVLTITAAAWVTVAQADVTVMAVRLQAKDVAALAKFYD
                     AAFGLKQIDQVGNPPTEIIMRYGATAAAAKAGTSPEFLIEKREPAVANDAMAHAIFHV
                     TDLAATLTAAKAAGGKMQGEVVTAPIGGIQVKIGTVVDPDGNVLELMELPKGVSTLPH
                     P"
     gene            3471..>3700
                     /locus_tag="VFL84_07815"
     CDS             3471..>3700
                     /locus_tag="VFL84_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608565.1"
                     /translation="MRLGHITSLAVAAGLLAGPALAQKMPDIGFKSVGRGRPLAASVL
                     DFDEEVGPNWIRRQGAQPGPDPKEPFPLNGYRP"
BASE COUNT          572 a         1209 c         1277 g          642 t
ORIGIN      
        1 gggcgacgcc aggaagacat acgccggggc aagctcggcg ggctgcgcgg gccgccgcat
       61 cggggagtcg gcgccgaagg tgatcggatc gtcctttgcc tcgcccgaca tcacaatcag
      121 cggcgtccag acggggccgg gcgccaccgc attgacgcga atgccctgtt tcacgaccga
      181 ttgcgcgagc gtcttcgtga acgcgttgat cgcgcctttc gtcgccgagt agtcgggcag
      241 cttctccgag ccgaggatgc cggtctcgga gctcgtcgcg atgatcgcgc tgcccggctt
      301 catgtggcgc agcgcggcgc gcgagagccg catgtaagca aagatgttcg tctcgaacgt
      361 gtgctgaagc tcctcgtcgc tgagctcgtc gaggctttcc ttgcggcgct gatacgcggc
      421 gttcgagacg agcacgtcga tgccgccgaa gtgctcgatg gttttgtcga tcaggtcctc
      481 gcacgtgtcg ggctcactca agtcgcccgg cagcagcagg cactcggcgc cggcttcctc
      541 gacggctgtt tgcgtctcct cggcgtcgga ttgctcttcg tcgaggtaat tgatcgcgac
      601 ggtcgcgcct tctcgcgcga acagcgtcgc gacggcgcgg ccgatgccgg agtccccgcc
      661 cgtgatcagc gccactttgc cctcgagctt gcccgccgcc ttgtattcgg gggcttcgaa
      721 ccggggcttc ggctcgagct tcgattcgag gcccggaggc ttttgtttct gcttggggaa
      781 cggcgggcgc ggctgcctag acgcggcttc cttcgcggat ttcttcttgg cctgcttggg
      841 ttgctgtttg cctctcacgg gacacctcct tgtgtctcaa gtgcgtgacg cagcacgaac
      901 catgccagcg ctcgaccggc ccgtcagtcg cggatctcga tccagacggg tgcgtggtcg
      961 ctggcttttt cacggccacg aacctcgcga tcgacgccgg cggccaagag cctcggcgcg
     1021 agcggcgggc tcaagagcag atgatcgatg cgcaagcccg cgtcgcgcgg aaacgcgttg
     1081 cgaaaatatt tccagaacgt gtagacgcgc tcgtcgggat gcagcgcgcg cacggcgtcg
     1141 acccagcctt gggccaacaa agtttcatag gccgagcgca cctcgggtcg gaacaacgcg
     1201 tcgtcgcgcc agcgctcggg tgcgtagacg tcgagatccg taggaatgac gttgaagtcg
     1261 cccgcgagaa cgacgggctt gctctcggcg agcagctccg cgccatggcg gatcagccgc
     1321 tcgagccagc gcagcttgta gtcgaagcgc gggccaggcg ccggattgcc gttcggcaga
     1381 tagaggcagc cgacgacgat gtcgccgacg gcggcctcga tgtagcggct gtgcagatcc
     1441 tcgggatcgc cgggcagcac gcgccgtctt tcgaccggag cgatcccgcg cgacaggatc
     1501 gcgacgccgt tccagctctt ctggccgtgc cagagcgcct cgtagccggc cgcgcgcagc
     1561 gcgtcggcgg ggaagcgctc ggagggcatc ttcagctcct gaaggcaggc gacgtcgggc
     1621 ttcgactctt cgagccagcg cgtgagcgcc gcgaggcgcg cgttgacgcc attgacgttg
     1681 aaggtcgcga tcctcacggc tggcgctcgc cgctgcacgg cccgtgccag cgaaggcaca
     1741 cggcttgcat gaatcggtcg tcgcgcgcgc gcgaatgcgg gatcgtcatg aggcgcctcg
     1801 ggatctggcc gttgatcaaa gcatcgatcg catcctggcg gaaggaccat gcctcgagca
     1861 tgggcggcgc gctggcctat tacacgctgt tctcgatcgc gcccgtgctg atcatcgtca
     1921 ttgccgttgc gggattcttc ctgggccccg aggccgcgcg cggagagatc gtcgcgcaac
     1981 tgcaagggct gctcggcccc gacggcgccg cggccgtcca agcgttgctc gagagcgcga
     2041 gcaagcccga ggaaggactg ctcgcaacga tcacgagcgt cttgctgctg cttctcggcg
     2101 cgacgacgat cttcgcggag ctgcagagcg acctcgatcg aatctggcgc gccgagacga
     2161 agccgatcag cggcctgtgg gcgttcctgc gcgcgcggat tttgtcgttc ggcatgattc
     2221 tcgtgctcgc gttcctgctg ctcgtgtcac tcgtagtcag cgcggcgctg tccgcgctcg
     2281 gcaagtggtg gggcggctgg ttcgaaggtt gggccgtgct gctcgaggtg ctgaacttcg
     2341 ccgtctcgtt cgcgatcacg acgggactct tcgcgctgat ctacaagctc ctgccgcgcg
     2401 ccgaaatcgc gtggcacgac gtgtggatcg gcgcggcggt cacggcgctc ttgttcgccg
     2461 tcggcaaatt tctaatcggc ctctatatcg gccgcagcgg cgtcgcctcg ggattcggcg
     2521 cggccggctc gttcgtcgtg ctgctcgtct gggtctatta ctcgacgcag atcttcctgc
     2581 tcggcgccga gttcacgtgg gtctacgcgc acgcgcgcgg ctcgaggcga gggcgggacc
     2641 gccccggcgc gcgcgagcgg gcaaccgcgg ccgcgtagaa gcgctcatcg acgacgactt
     2701 cgcgccgatg gtcaggtccg aggacgaccg gtcaaaggcg tcttttcggc gtatcatcca
     2761 gcggcgcccg gagaaggcgt ccgggcggcg tcgaaacact tccaggagaa caagacatgc
     2821 ggcgagcgat cacggcggta ctcacgatca ctgcggcggc ttgggtgacg gtggctcagg
     2881 ccgacgtcac ggtgatggcc gttcggctgc aagcgaaaga cgtggccgcg ctcgcgaagt
     2941 tctacgacgc ggcgttcggc ttgaagcaga tcgatcaggt cggcaacccg ccgacggaaa
     3001 tcatcatgcg ctacggcgcg acggccgccg ccgccaaggc cggcacgtcg cccgagttcc
     3061 tgatcgagaa gcgcgagccc gccgtcgcca acgacgcgat ggcgcacgcg atcttccacg
     3121 tcacggatct cgccgcgacg ttgacggccg cgaaagccgc gggcggcaag atgcagggcg
     3181 aagtagtgac ggctccgatc ggcggcatcc aggtcaagat cggcaccgtc gtcgatcccg
     3241 acggcaacgt gctcgagctc atggagctgc cgaagggcgt cagcacgctg ccgcacccgt
     3301 aacggctgcg acgccgcttg tgctgcgcga ccccgcccgc ggtcgcgcgc gggcgcctag
     3361 tgcgcgagag aaaacttttg acgggcccgc accggtccgg gtgctcaggc tcatatagaa
     3421 ggtgggtaca gtagcggtct gacgcacgta acccccgtca gagggacacc atgagactcg
     3481 gacacatcac atcgttggcg gtcgccgccg gtctgctcgc cgggccggct ttggcccaga
     3541 agatgccgga catcggcttc aagagcgtcg gccgcggccg gccgctcgcc gcgagcgtgc
     3601 tcgacttcga cgaagaggtg ggcccgaact ggatccgtcg gcaaggcgcg cagcccggcc
     3661 cggacccgaa ggagccgttc ccgttgaacg gctaccgacc
//
LOCUS       DASSNN010000418         4766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1086303, whole genome shotgun sequence.
ACCESSION   DASSNN010000418 DASSNN010000000
VERSION     DASSNN010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086303"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VFL84_07820"
     CDS             <1..698
                     /locus_tag="VFL84_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET7608566.1"
                     /translation="AGQDAVNSTRAFHEALPLTGVILTKTDGDARGGVALSVREITGL
                     PIKLVGTGEKVDALETFVPARFASRILGMGDVVGLVEQVQKTVDREEMEKLAGKVKQG
                     KGLSLEDYREQLKQMVNMGGLEQILDKSPGVKPEQLSAAKFDPKVFKRQIGIIDSMTP
                     KERRHPDLIDGSRKRRIAAGAGLPIQDVSRLLKQHKQLTKTMKQLAKGGGMQRLLGAM
                     RGGGPPPFLRGRR"
     gene            802..1059
                     /gene="rpsP"
                     /locus_tag="VFL84_07825"
     CDS             802..1059
                     /gene="rpsP"
                     /locus_tag="VFL84_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020031871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET7608567.1"
                     /translation="MVVIRLARAGTVKRPFYHVVVADRRRARGGRYIERIGFFNPIAT
                     GGEERLRVDLARVDHWVAQGAKPSERVTDLLKQYRANAKAA"
     gene            1087..1596
                     /gene="rimM"
                     /locus_tag="VFL84_07830"
     CDS             1087..1596
                     /gene="rimM"
                     /locus_tag="VFL84_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842306.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET7608568.1"
                     /translation="MSAEQKWVILGRVSGVFGVKGWLKVQSYTEPRDNIVGFDAWTLR
                     MNGVDHSFEVEEGHSHAGSVVAKLRGLDDRDLARDFVGAEIVVPRERLPTIAPDELYW
                     TDLEGLEVRTTAGIVLGNVDHLLATGGNDVLVLDSSPQRLIPFVVGSVVKEVDLKRKL
                     IVVDWSPDY"
     gene            1607..2365
                     /gene="trmD"
                     /locus_tag="VFL84_07835"
     CDS             1607..2365
                     /gene="trmD"
                     /locus_tag="VFL84_07835"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET7608569.1"
                     /translation="MYRFGVISLLPELVAPVMETGVVGRAAERGLVALEQVSPREFTT
                     DRHRTVDDRPYGGGPGMVLKYEPLRDAVLELRRRLPAGTREFVLTAQGRPFDQALARE
                     LAAAPGLLLVCGRYEGIDERFTEAVVGAELSLGDYVLSGGELAAAVVIDAVTRLLPGV
                     LGDECSHVEDSFTAGLLDCPQYTRPEVADGCRVPEVLLGGDHEAIRRWRLKQALGRTW
                     LRRPELLEKRQLTSEERMLLEEFESEWSELNSSG"
     gene            2429..2773
                     /gene="rplS"
                     /locus_tag="VFL84_07840"
     CDS             2429..2773
                     /gene="rplS"
                     /locus_tag="VFL84_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET7608570.1"
                     /translation="MSRVIDQLEQQQLKQDVPDFAPGDTVVVQVRVTEGDRERLQAYE
                     GIVIAKRNRGLNSSFTVRKVSHGEGVERVFQTHSPAVAEIKVKRRGDVRRAKLYYLRD
                     LSGKAARIKEKV"
     gene            2868..3647
                     /locus_tag="VFL84_07845"
     CDS             2868..3647
                     /locus_tag="VFL84_07845"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HET7608571.1"
                     /translation="MIGGIAADTLSAAILNQDDPALVESGVPAYLLLIDGLISEHPDN
                     VALLSAGAQLFALYGSRFAPPERGITLTAKARRYGERAICLAHEPACHWADADYTQFV
                     EALQDVDDKDIGPLYSYAVSWLSHLDATSEDWTAVAELPWVEAVLERALALDETYEDG
                     AVHGYLGILNSLRPPALGGKPDVAREHFERAIELSGGRDLSIKVEYARRYARLVFDQE
                     LHDRLLTDVLNAPVEAPGKTLFNVLAKQEAETLLASSKEYF"
     gene            3647..4672
                     /gene="dctP"
                     /locus_tag="VFL84_07850"
     CDS             3647..4672
                     /gene="dctP"
                     /locus_tag="VFL84_07850"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET7608572.1"
                     /translation="MRDCRAANTRSFARLLLGALLAAVGAAANAADIKIATVAPDGSR
                     WMQQMRAAAEEVNARTSGRVTLKFYPGGVMGNDAQVLRKLRIGQLQGGAFTAGGLAER
                     YPGLNLYGIPLLFRSLDEVDAVRAQLDPELGAGLDRAGFVTFGFIEGGFANLLSNEPI
                     RSVEDMRRKKVWVPEGDAISFLAMETMGLSPSVLPVTDVLTGLQTGLIEITFASPVAA
                     LVLQWHTKVKYITDLPISYSMGVVAIEKGAFSALSADDQRVVREVMGRYCHQLDREAR
                     DDNRKAAEVLAQSGLQTVTVDAADVAGWRKTIEAIHPQLRARPDIDAALFDRLLVLLA
                     EYRRAHP"
BASE COUNT          798 a         1529 c         1659 g          780 t
ORIGIN      
        1 tggcaggcca agacgccgtg aactcgacgc gcgcgttcca cgaggcgctg ccgttgacgg
       61 gcgtcatcct gacgaagacc gacggcgacg cgcgcggcgg cgtcgcgctg tcggtccgcg
      121 agatcacggg attgccgatc aagctcgtcg gcaccggcga gaaggtcgac gcgctcgaga
      181 cgttcgtgcc cgcgcggttc gcctcgcgga tcttaggcat gggagacgtc gtcgggctcg
      241 tcgagcaggt gcagaagacc gtcgaccgcg aggagatgga gaagctcgcc ggcaaggtca
      301 agcaaggcaa gggcctgagc ctcgaggact atcgcgagca gctcaagcag atggtgaaca
      361 tgggcggcct cgagcagatc ctcgacaaga gcccgggcgt gaagcccgag cagctgtcgg
      421 cggccaaatt cgacccgaag gtcttcaaac gccaaatcgg catcatcgac tcgatgacgc
      481 cgaaggaacg gcgccatccc gacctcatcg acggctcccg caaacggcgg atcgcggcgg
      541 gggccggctt gcccatccag gacgtcagcc ggctcttgaa gcagcacaag cagctcacga
      601 agaccatgaa gcagctggcg aaaggcggcg gcatgcagcg cctgctgggc gccatgcggg
      661 ggggtggccc cccgccgttc ctgcgggggc ggcgctaggc gcccggggcc ttacgctcga
      721 cgggcgaact caagtagaat tcgccccctc aacgggcggc ccactcgggc cgccttggtt
      781 ttttcggaag cggtgggttt tatggtcgtc attcggctgg cgcgtgccgg cacggtcaag
      841 cgtcctttct atcacgtcgt cgtggccgac cggcgtcggg cgcgcggcgg gcgttacatc
      901 gagcgcatcg ggttcttcaa tccgatcgcc acgggcggcg aggagcggct gcgcgtcgat
      961 ctcgcgcgcg tcgaccactg ggtcgcgcaa ggcgccaagc cttcggagcg ggtcacggac
     1021 ctgctcaagc aataccgcgc gaacgccaag gcggcgtagc gcgccgcagc gccctgcggc
     1081 accgcagtga gcgcggaaca gaagtgggtc atcctcggcc gcgtcagcgg cgtattcggc
     1141 gtcaaagggt ggctcaaagt gcagtcgtac acggagcctc gcgacaacat cgtcgggttc
     1201 gacgcgtgga cgctgcgcat gaacggcgtc gatcactcct tcgaagtgga ggaagggcat
     1261 agccacgctg gcagcgtggt cgcgaaattg cgcggcctcg acgatcgcga tctcgctcgc
     1321 gacttcgtag gtgcggagat cgtcgtcccg cgcgagcggt tgcctacgat cgcgccggac
     1381 gagctctact ggacggacct cgaagggctc gaggttcgca cgacggccgg catcgtgctc
     1441 ggcaacgtcg atcatttgct cgcgacgggc ggcaacgacg tgctcgtgct cgacagctcg
     1501 ccgcagcgtc tgatcccgtt cgtcgtcggt tccgtagtca aagaggtcga cctgaagcgc
     1561 aagctcatcg tcgtcgactg gtcgcccgac tactgagggc acgacgatgt atcggtttgg
     1621 cgtgatctcg ttgttgccgg agctcgtcgc gcccgtcatg gagacgggcg tcgtcggacg
     1681 cgccgccgag cgcgggctcg tcgcgctcga gcaggtgagc ccgcgggagt tcacgacgga
     1741 tcggcaccga accgtcgacg accgtccgta cggcggcggc ccgggcatgg tgttgaagta
     1801 cgagccgttg cgcgacgcgg ttctggagct gcggcgccgc cttccggccg gcacgcgcga
     1861 gttcgtgttg acggcgcaag gcaggccgtt cgaccaagcg ctcgcgcggg agctcgcggc
     1921 ggcgccgggg ttgctgctcg tttgcggacg ctacgaaggc atcgacgagc gcttcacgga
     1981 ggccgtggtc ggcgcggagc tgtcgctcgg cgactacgtg ctctcgggag gtgagctcgc
     2041 cgcggccgtc gtgatcgacg cggtgacgcg gctcttgccg ggcgtgctcg gcgacgaatg
     2101 ctcgcacgtc gaggactcgt tcacggcggg cttgctcgat tgcccgcagt acacgcgccc
     2161 cgaggttgcg gacgggtgcc gagtgcccga agtgctgctc ggcggcgatc acgaagccat
     2221 ccggcggtgg cggctgaagc aagccctcgg gcgcacgtgg ctcaggaggc ccgagctgct
     2281 cgaaaagcgg cagctcacga gcgaggagcg aatgttattg gaggagttcg agtcggaatg
     2341 gtccgagctg aactcgagcg gctagattgc gtagaatagt gtgagatcgc gcaacggcct
     2401 acgaacggga atgacagagg atgtacgtat gtccagggta atagaccaac tagaacagca
     2461 acagctgaag caagacgttc ccgactttgc gccgggcgac accgtcgtgg tgcaagtgcg
     2521 cgtcactgaa ggagatcgcg agcggctgca ggcctacgaa ggcatcgtga tcgcgaagcg
     2581 gaaccggggg ctcaactctt cgtttacggt cagaaaggtt tcgcacggtg aaggcgtcga
     2641 gcgcgtgttt cagacgcaca gccccgccgt cgctgaaatc aaggtgaaac gacgtggcga
     2701 tgtccgccgg gccaagctct attacctgcg cgatctcagc ggcaaggcag cccgcatcaa
     2761 agaaaaggtc taacgcgttc ggcgtagctc tcgcacgcgt cggctcgatc gtctcgaggc
     2821 tcgcgtgcgc cggcgccgcc gcggcgttcg cgacgggatg cacgaccttg atcggcggca
     2881 tcgccgcgga cacgctttcc gccgcgatcc tgaatcagga cgacccggct ctcgtcgaga
     2941 gcggcgtccc cgcgtacctg ttgctgatcg acggcctgat ctcggagcac cccgacaacg
     3001 tcgcgctgct ctcggcgggc gcgcagctgt ttgcgctcta tggctcgcgc ttcgcgccgc
     3061 cggagcgcgg catcacgttg acggcgaagg cgcgacgcta cggtgagcgc gccatctgtc
     3121 tcgctcacga gccggcttgc cactgggccg acgccgacta cacgcagttc gtcgaggcgc
     3181 ttcaagacgt cgacgacaag gacatcggac ctctctactc gtacgccgtg tcctggctga
     3241 gccatctcga cgccacgagc gaggactgga cggccgtcgc cgagcttccg tgggtcgagg
     3301 ccgttctcga gcgcgcgctc gccctcgacg agacgtacga ggacggtgcc gtgcacggtt
     3361 acctcggcat tctgaattcc cttcggccgc cggcgctcgg cggcaagccg gatgtcgcgc
     3421 gcgagcactt cgagcgcgcg atcgagctct cgggcggccg cgatctctcg atcaaggtag
     3481 aatatgcgcg tcgctacgcg cgcctcgtat tcgaccaaga gctgcacgac cggctcttga
     3541 cggacgtgct gaatgcgccg gtcgaggcgc cgggcaagac actgttcaac gtgctggcga
     3601 aacaggaagc agagactttg ctcgcgtcct caaaggagta tttctgatgc gcgattgccg
     3661 agccgccaac actcgctcgt tcgcgcggct cttgctcggc gctctgctcg ccgcggtcgg
     3721 agcggcggcg aacgccgccg acatcaagat cgcgaccgtg gcgccggacg gctcgcgctg
     3781 gatgcagcag atgcgcgcgg ccgccgaaga ggtcaacgcc cgcacttcgg gccgcgtgac
     3841 gctgaagttc tatcccggcg gcgtcatggg caacgacgcg caggtgctgc gcaagcttcg
     3901 gatcggccag ctgcagggcg gcgccttcac ggccggcggg ctcgccgagc gctacccggg
     3961 cctgaacttg tacggcatac cgctgctgtt ccgctcgctc gacgaggtcg acgccgtgcg
     4021 cgcacagctc gatccggagc tcggagccgg tctcgaccgg gccgggttcg tgacgttcgg
     4081 cttcatcgaa ggcggctttg cgaatctgct ttccaacgag ccgatccgca gcgtcgagga
     4141 catgcggcgc aagaaggtct gggtgccgga aggcgacgcc atcagcttcc tcgctatgga
     4201 aacgatgggg ctttcgccgt ccgtgctgcc cgtgaccgac gtgttgacgg ggctgcagac
     4261 cgggctcatc gagatcacgt tcgcgtctcc ggtcgcggcg ctcgtgctgc agtggcatac
     4321 caaggtcaag tacatcaccg acctgccgat ctcctactcg atgggcgtgg tggcgatcga
     4381 gaagggcgcc ttctccgcgc tcagcgcgga cgatcagcgc gtggttcgcg aggtcatggg
     4441 gcgctactgc catcagctcg accgcgaagc gcgcgacgac aatcgaaaag cggccgaggt
     4501 cctcgcgcag tccgggttgc agaccgtgac cgtcgacgcc gccgacgtcg cgggctggcg
     4561 caagacgatc gaggccattc atccgcagct gcgcgcgcgt cccgacatcg acgcggcgct
     4621 gttcgaccgt ctgctcgtgc tgctggccga atatcggcgc gcgcatccct agagcgctgc
     4681 cccaccgcct ttgagcgacg ccgcacagaa ccgttggtgg gctcgagtcg accgcgttgc
     4741 tcgcaatgtc gagacggcgc tgatcg
//
LOCUS       DASSNN010000419         3935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_577936, whole genome shotgun sequence.
ACCESSION   DASSNN010000419 DASSNN010000000
VERSION     DASSNN010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577936"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1083)
                     /gene="xerD"
                     /locus_tag="VFL84_07855"
     CDS             complement(181..1083)
                     /gene="xerD"
                     /locus_tag="VFL84_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003209584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET7608573.1"
                     /translation="MSLPSDPKALVDRFLDAIWMERGLSENTLGAYRADLLALNQRLQ
                     TRNVELARAARADVLDYIAWRVEAGAKPRSTARQLSSFRRFYRYLLREGLLSEDPTAQ
                     IAMPKIGRALPQSLTEEEVEALLAAPVVAEPLGHRDRAMLEVLYATGVRVSELINLKM
                     SQVNLNQGVLRIVGKGDRERLIPLGDEAQDWVKEFVNGPRVEILLERQTEYLFPTRRG
                     DRMTRQAFWHIIKRYARKAGVKKKLSPHTVRHAFATHLLNNGADLRVVQLLLGHSDVS
                     TTQIYTHVARERMKELHSRHHPRG"
     gene            1412..2149
                     /locus_tag="VFL84_07860"
     CDS             1412..2149
                     /locus_tag="VFL84_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7608574.1"
                     /translation="MTRIVHCAYGCYAWVALLAIVIPLCIVLAVMPGLERRRRLARAA
                     ARAFFFAIGSSVRVEGSDIEPHYPCVVVANHSSYLDGIILTAALPAGFTYLIKNEMSR
                     MPIAGFLLRRLGSAFVDRDDRRDRHRTARALLSLATRGSALGFFPEGTFDKAPGLKAF
                     HLGAFSAAARAKLPVVPVVIHGARWKLPSGRFLPRPGPLRVHVCEAVRLPPASPSPQA
                     LMAETRRAMLRHLDEPDLSPDAGYIPE"
     gene            complement(2134..3786)
                     /locus_tag="VFL84_07865"
     CDS             complement(2134..3786)
                     /locus_tag="VFL84_07865"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HET7608575.1"
                     /translation="MSRESMELDVVVVGAGPAGLATACRLAQLARAAGRELQIGVVEK
                     GAAVGAHIVSGAVFEPRALDELFPDWRERGAPVKTPVTAERVEWLASAKRSIRVPELL
                     VPRALRNHGNFIISLGDLCQWLAGQAESLGVNVLPGFAAADLLYDGERVAGVVTGDLG
                     IARDGSHKPTYQPGYELRAKYTVLAEGCRGHLGGRVEARFALRAGADPQHYAIGFKEI
                     WQVDAAKHRLGEIVHTLGWPLDAATDGGGFVYHAADARVYLGLVVSLAYRNPHLDPFA
                     EFQRWKQHPAIRLLLARGERIAYGARAVNKGGLQSLPKLTFRGGLLVGCEAGFLNPAK
                     IKGSHAALKSGMLAAETIFAALALEGAAAEAELARYDERVRESWLWDELRRGRNFSAG
                     VAKLGTFLGGALAFVEQNLLQGRAPWTLHNRVPDHARLREAAHAPRPRYARPDGVVAF
                     DRSSSVFLSGTAHEENQPSHLKLADPSVPIAVNLPRYDEPAQRYCPAGVYEVVADSSG
                     APCFRINATNCVHCKTCDIKDPAQNITWVPPEGGGGPNYSGM"
BASE COUNT          613 a         1349 c         1321 g          652 t
ORIGIN      
        1 cgtcagctcc tgcgccggcg cggcgccggc ggcgaggccg agcggcaggc cgatcagggc
       61 agctagagcg gggtatttcg gcacgggcag tctcctacct tcgcaggcgc ttcctctcga
      121 cggattctac caataaagac cccagcggga agcgaaagac ccctacgcgg aaaacccgga
      181 tcagccgcgc ggatggtggc gactatgcag ctctttcatg cgctcgcgcg cgacgtgtgt
      241 atagatttgt gtagtcgaca cgtcgctgtg gccgaggagc agctgcacga cgcgtaaatc
      301 ggcgccgttg ttgagcaggt gcgtcgcgaa ggcgtggcgg accgtgtgcg gcgagagctt
      361 ctttttgacg ccggccttgc gcgcgtagcg cttgatgatg tgccagaagg cctgccgcgt
      421 cattcggtcg ccgcggcgcg tcgggaacag gtattcggtc tggcgctcga gcaggatctc
      481 gacgcgcgga ccgttcacga attccttgac ccagtcctgc gcctcgtcgc cgagcggaat
      541 gagccgctcg cgatcgccct tgccgacgat gcgcagcacg ccctgattca ggttgacctg
      601 actcattttc agattgatga gctcggacac gcgcacgccc gtggcgtaca gcacttcgag
      661 catcgcgcga tcgcggtggc cgagcggctc cgcgacgacc ggcgccgcga gcaaggcctc
      721 gacctcttcc tcggtcagcg attgcggcaa cgcgcggccg atcttcggca tcgcgatctg
      781 cgccgtcggg tcctcgctga gcaaaccctc gcgcagcaag tagcgataga agcggcggaa
      841 gctcgacagc tgtcgcgccg tcgagcgcgg tttcgcgccg gcttccacgc gccacgcgat
      901 gtaatcgagc acgtcggcgc gcgccgcgcg ggcgagctcg acgttgcgag tctgcaggcg
      961 ctggttcagt gccagcagat cggcgcgata cgcgccgagc gtgttctccg agagcccgcg
     1021 ctccatccaa atcgcatcga gaaagcgatc gacgagcgct tttggatcgc taggtagact
     1081 catgcctgcc tgagccgggc cgctgtgcag aaagttgtgc gccattgccg ctacgcgatc
     1141 gtatgttccg gtgctttcgt atacaattca acgccttaga cgacacgctg cggcacgcgc
     1201 aaaaggcatg ctgcagcggg tcgagtatgc gtgacgggtg cgtccccctg cgtgaccgcc
     1261 ttcacagatg tttagcagca ttaacataaa gagaagcgtg tcacagaaac cggacgcgaa
     1321 cccgtttcga atgtcgctcc ctggcgacgg tagcgttgac ataaacgtcg aacggacctc
     1381 acggatccgc agcggaacac cgctgtcggc cgtgactcgc atcgtccatt gcgcctacgg
     1441 ctgctacgcc tgggtggcct tgctcgccat cgtaatcccg ctctgtatcg tcctggccgt
     1501 catgccgggc ctcgagcggc gtcgccgcct cgcgcgagcc gcggcgagag cgttcttttt
     1561 cgcgatcggc agctcggtcc gcgtcgaggg cagcgacatc gagccgcact acccgtgcgt
     1621 cgtcgtcgcg aatcactcga gctatctcga cggcatcatc ctgaccgccg cgctgccggc
     1681 gggcttcacg tatttgatca aaaacgagat gagccgaatg ccgatcgcgg ggttcttgct
     1741 gcggcggctc ggcagcgcgt tcgtcgaccg cgacgaccgc cgcgatcggc atcgcacggc
     1801 gcgcgcgctg ctctcgcttg cgacgcgcgg ctcggcgctc gggttctttc ccgaaggcac
     1861 gttcgacaag gcgcccgggc tcaaggcgtt tcatctcggc gcattcagcg ccgcggcgcg
     1921 cgcgaagctg cccgtcgtgc cggtcgtgat ccacggggcg cgctggaagc tgccgtcggg
     1981 ccggttcttg ccgcgcccgg ggccgttgcg cgtccatgtc tgcgaggccg tccggctgcc
     2041 gccggcgagc ccctcgccgc aggcgctgat ggccgagacg cgccgtgcga tgctccggca
     2101 cctcgacgag cccgatctct cgcccgacgc gggctacatt cccgagtagt tcgggccccc
     2161 gcccccttcg ggcggcaccc acgtgatgtt ctgcgcgggg tccttgatgt cgcaggtctt
     2221 gcagtgcacg caattcgtcg cgttgatgcg aaaacacggc gcgccgctcg agtcggcgac
     2281 gacctcgtag acgccggccg ggcagtagcg ctgcgcgggc tcgtcgtagc gcggcaagtt
     2341 cacggcgatc ggcacgctcg ggtccgcgag cttgagatgc gagggctgat tctcttcgtg
     2401 cgccgtgccc gacaagaaca ccgagctcga tcggtcgaac gccacgacgc cgtcgggcct
     2461 cgcgtagcgc ggccgcggcg catgcgcggc ttcgcgcaag cgcgcgtggt cgggcacgcg
     2521 gttgtgcagc gtccacggcg cgcggccttg caacaggttc tgctcgacga acgcgagcgc
     2581 gccgcccaag aacgtgccga gcttcgccac gccggcgctg aagttgcggc cgcggcggag
     2641 ctcgtcccag agccacgact cgcgcacgcg ttcgtcgtag cgcgcgagct ccgcttcggc
     2701 cgccgcgcct tcgagcgcga gcgcggcgaa gatcgtctcg gcggccagca tcccgctctt
     2761 cagcgcggcg tgcgagcctt tgatcttcgc cgggttcagg aacccggcct cgcagccgac
     2821 gagcagcccg ccccggaacg tcaacttggg cagcgactga agaccgcctt tgttgacggc
     2881 gcgcgcgccg tacgcgattc gctcgcctcg cgcgagaagc agacggatgg cggggtgctg
     2941 tttccaccgt tggaactcgg cgaacggatc gagatgcgga ttgcgatacg cgaggctgac
     3001 gacgaggccc aagtaaacgc gggcatcggc ggcgtgatag acgaagccgc cgccgtcggt
     3061 cgcggcatcg agcggccacc ccagcgtgtg cacgatctcg cccaagcggt gcttcgccgc
     3121 gtcgacttgc cagatctcct tgaagccgat cgcgtaatgc tgcgggtcgg cgcccgcgcg
     3181 caaagcgaag cgcgcttcga cgcggccgcc gagatggcct cgacagcctt cggcgagcac
     3241 ggtgtatttc gcgcgcagct cgtaccccgg ctgatacgtc ggcttgtgcg agccgtcgcg
     3301 cgcgatgccg agatcgccgg tcacgacgcc cgcgacgcgc tcgccgtcgt agagcaggtc
     3361 ggccgcagcg aaccccggca gcacgttgac gccgagcgat tccgcttgcc cggccagcca
     3421 ctggcagaga tcgcccaagc tgatgatgaa gttgccgtga ttgcgcaagg cgcgcggcac
     3481 gaggagctcg ggaacgcgga tgctgcgttt cgcgctcgcg agccactcga cgcgctcggc
     3541 cgtcacgggc gttttgacgg gcgcgcctct ttcgcgccaa tccgggaaca gctcgtcgag
     3601 cgcgcgcggc tcgaagaccg cgccggaaac gatgtgcgcg ccgacggccg cgcccttctc
     3661 gacgacgccg atttgcagct cgcggcccgc cgcgcgcgcg agttgcgcca atcgacacgc
     3721 cgtcgcgaga ccggccgggc ctgcgccgac gacgacgaca tcgagctcca tgctttcgcg
     3781 tgacattggc ggcgcgcccg cgccgccgta cggcgcgaac acgcggcaca ttgtaacatc
     3841 gggatcgagc cgcccttctc cgcgaccacg tgagcacacc gatctggcaa cctagtcccg
     3901 cgcgctgcga cgcaagcaac atgcggcgct tcatc
//
LOCUS       DASSNN010000420         3122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1205182, whole genome shotgun sequence.
ACCESSION   DASSNN010000420 DASSNN010000000
VERSION     DASSNN010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205182"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..889)
                     /locus_tag="VFL84_07870"
                     /pseudo
     CDS             complement(47..889)
                     /locus_tag="VFL84_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661056.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1106..1978
                     /locus_tag="VFL84_07875"
     CDS             1106..1978
                     /locus_tag="VFL84_07875"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7608576.1"
                     /translation="MCEKDHFEQDLEEAIRSGRVTRRDFGGLAAGIGLAVVLPRAANA
                     QAVTENTVEIKTPDGTCDAHFAYPQSGAHAAVVVWPDILGLRPAFEQMGKRLAESGYS
                     VLTVNPFYRGKRAPVLPPGSAFNAETRAIVGPLAQQLNATTHTNDAKAFIAWLDQQPQ
                     VDKNRKIGTTGYCMGGPITMRTAAAVPNRVGAAASFHGGGLVTQMPDSPHLLVPQMKA
                     QYLFAVAQNDDMREPEAKNVLKESFAKAKLDAEIEVYPAMHGWCPPDSQVYDMAQAEK
                     AWSRMLVLFERALA"
     gene            complement(2066..>3122)
                     /locus_tag="VFL84_07880"
     CDS             complement(2066..>3122)
                     /locus_tag="VFL84_07880"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HET7608577.1"
                     /translation="YVAAAFPSACGKTNFAMLIPPRSFSGWKITTVGDDIAWIKRGPD
                     GELRAINPEAGFFGVAPGTSEKTNPNAIATLRANVIFTNVALTDDGDVWWEGMTSEPP
                     AHLVDWTGADWTPELARQTGRKAAHPNARFTVPAAQCPSIDADWEKPSGVPVSAFVFG
                     GRRATTVPLVYEAFNWNFGVYLAATLGSETTAAAEGKQGVVRRDPFAMLPFCGYHIGD
                     YFNHWLRIGRTLSDKPRIFCVNWFRVDAKGVFLWPGFGDNMRVLKWIVDRVRGRAAGR
                     ETELGWVPRYDDIDWTHCAVTREAFDELIAVDNDAWHKELAQHGDWFAKLGSRLPQQL
                     KIKYDLLVARFSHAPAA"
BASE COUNT          556 a          994 c         1010 g          562 t
ORIGIN      
        1 gccatggaag agatcgcggc gggcttcaac gagcagatcc gcatcttcaa ggcgcgcggc
       61 taagcggtaa gatcgcggct tgacatgctc cccgccctgg agggccggag gttcccacag
      121 ctggcgagtg atgtcccact cggagaatgt ccgacgcagc gttttggtca cgatcatgta
      181 cggaaccgca gtcaccacac gtctagcgca gcatgtttcg gagctcgttc cgcgcatttt
      241 tgatgcgcgc gtggatgttg gtagcgaggc tttgcttgtt ggcgcgctgc gccaaggcca
      301 acttgccggc aagcttggcg agttgcttgc ggttctcgat cttctcgcca gtcgagagtg
      361 ccgcaagatc cttcagtccg agatcgatgc ccactgggcg ctcgatcgct cgcggctcaa
      421 gatcgggtat ctcgacgacg atgttcaggt atcagcgctt gcgcgcgtcc tgagcgaagt
      481 tcgtgccgtc tttgatcttg ccttgcggca gcggtcgcga gtaccagacg cggaaagtat
      541 tgccggcgaa ccggaacgcg ttcccttcgc gcttgaggtc tcggcctttc agcggtaccc
      601 agccgaggtt gtggcgcccg cgatagcgca agaacggtct tttgtgctgt tggcgagact
      661 tggcgtattg ctcgcagacg gcgttgacgg cgcccgagtg aaggccgagc tccttgctcg
      721 atccggtcgt gagaacgttc agatcgaagc cgctgggcca tggcttgttc caggtcagcg
      781 cggacttctg cacattgttg cagtagttcc agacgcagtt gactgcgagc gcttgctggt
      841 gaagcagacc cgcgagattc tttacgcggt agcggtagac ctgaatcatt ctcggtacta
      901 tcgcattggg ctcaagcaat acgtcaagga gcagcgcgcg tccacgcctc cgtaacgacc
      961 tacacggctg agcggcgctc cctcccggtg aacgacgcgg ttttcaggcc gaagactcga
     1021 tgagccggtg acgaagagtc ggtatagtcg gatggaccgg ggcgccgact ttcggggcgc
     1081 ctcataacaa agggggaacg ccggcatgtg cgagaaggat catttcgaac aagacctcga
     1141 agaagccatc cgttcagggc gcgtcacacg ccgcgacttc ggcggcttgg ccgcaggcat
     1201 cggcttggcg gtggtgttgc cgcgcgcggc gaacgcgcag gccgtgacgg agaataccgt
     1261 cgagatcaag acgcccgacg gcacttgcga tgcgcatttc gcgtatccgc aaagcggcgc
     1321 gcacgccgcc gtcgtcgtat ggcccgacat cttggggctg cgcccggcgt tcgagcagat
     1381 gggcaagcgg ctcgccgaat cgggctactc ggtgttgacc gtgaatccgt tctaccgcgg
     1441 caagcgagcg cccgtgctgc cgcccggctc ggcattcaac gcggagacgc gcgccatcgt
     1501 cggtcctctc gcgcagcagc tcaacgcgac gacgcacacg aacgacgcga aagcgttcat
     1561 cgcctggctc gatcagcagc cgcaagtcga caagaaccgc aaaatcggca cgaccggcta
     1621 ctgcatgggc gggccgatca cgatgcgcac ggcggcggcc gtcccgaacc gcgtcggcgc
     1681 cgcggcttcg ttccacggcg gcggcctcgt tacgcaaatg cccgacagcc cgcacttgct
     1741 cgtgccgcag atgaaggcgc agtacttgtt cgccgtcgcg cagaacgacg acatgcgcga
     1801 gcccgaagcg aagaacgtgc tgaaagagtc gtttgcgaaa gcgaagctcg acgccgagat
     1861 cgaggtgtac ccggccatgc acggctggtg cccgcccgac tcgcaggtct acgacatggc
     1921 ccaggccgaa aaagcctgga gccgcatgct cgtcctcttc gagcgcgccc tcgcctaacc
     1981 ccaagaggtg ccggtgttac ggtttcggtt caaccgtaac tccggcactt cggttgaacc
     2041 gaaaccgtaa caccggcacc ttcggttagg ccgctggggc gtgcgagaac cgcgcgacga
     2101 gcaggtcgta cttgatcttc agttgttgcg gcagccgcga gccgagcttc gcgaaccagt
     2161 cgccgtgctg cgcgagctct ttgtgccaag cgtcgttgtc cacggcgatg agctcgtcga
     2221 acgcttcgcg cgtcactgcg caatgcgtcc agtcgatgtc gtcgtagcga ggcacccagc
     2281 cgagctcggt ctctcgcccc gcggcgcggc cacgcacgcg gtcgacgatc cacttcagca
     2341 cgcgcatgtt gtcgccgaag cccggccaca gaaacacgcc cttggcgtcg acgcggaacc
     2401 agttcacgca gaagatacgc ggcttgtcgc tcaaggttcg gccgatccgc agccagtggt
     2461 tgaagtaatc gccgatgtga taaccgcaga acggcagcat cgcgaacgga tcgcgccgaa
     2521 cgacgccttg tttaccttcg gcggccgcgg tcgtctcgga tcctagcgtc gccgcgagat
     2581 agacgccgaa attccaattg aacgcctcgt agacgagcgg caccgtcgtc gcgcggcgcc
     2641 cgccgaagac gaacgccgag accggcacgc cgctcggctt ctcccagtct gcgtcgatag
     2701 acgggcattg cgccgccggc accgtgaacc gcgcgttcgg atgggctgcc ttgcggccgg
     2761 tctgccgtgc gagctcgggc gtccaatcgg cgccggtcca gtcgacgagg tgcgcgggcg
     2821 gctcgctcgt cataccttcc caccagacgt cgccgtcgtc ggtcagcgcg acgttcgtga
     2881 agatcacgtt tgcgcgcagc gtcgcgattg cgttcggatt cgtcttctcc gaagtaccgg
     2941 gggcgacgcc gaagaagccg gcctccggat tgatcgcgcg cagctcgccg tccgggccgc
     3001 gcttgatcca cgcgatgtcg tcgccgacag tcgtgatttt ccagcccgag aacgagcgcg
     3061 gcggaatcag cattgcaaag ttcgtcttgc cgcacgcgga cgggaacgcg gcggcgacgt
     3121 ag
//
LOCUS       DASSNN010000421         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_815684, whole genome shotgun sequence.
ACCESSION   DASSNN010000421 DASSNN010000000
VERSION     DASSNN010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815684"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VFL84_07885"
     CDS             complement(<1..167)
                     /locus_tag="VFL84_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET7608578.1"
                     /translation="MDLAQVSTSLALLVVLALAFDFMNGFHDAANSIATVVSTGVLKP
                     WQAVIWAAFFNV"
     gene            complement(189..830)
                     /locus_tag="VFL84_07890"
     CDS             complement(189..830)
                     /locus_tag="VFL84_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HET7608579.1"
                     /translation="MNFIARIMPREGRFFALFDGHAKLIVDGAVALAEVLRHYEVQQR
                     RDAGIKLIEDVEHSADRITHETVQLMHTTFVTPFDRDDIHRLISRMDDVLDLIQDAAE
                     SLVLYDIQKVTPEATQLAELVLRCAERVQAAVKLMASMAEAPAMLEICLEIDKLESEA
                     DKVMRGAISRLFRDETDVRQVIKLKAVYEALESATDKCQDVANVIESVVLDNG"
     gene            898..>2212
                     /locus_tag="VFL84_07895"
     CDS             898..>2212
                     /locus_tag="VFL84_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7608580.1"
                     /translation="MGRVVVLVAALLIGAGAHAQEVALSFTAAQAAQGRAVYDTTCAM
                     CHGANLDDGPLGAPLKGDAFMRKYGGKSARALYDVLRTTMPTGNPGSLSAETYAALAA
                     LIFSQNSIVAGATPLPADPQLLAAMQVPAGGFSFMAFSPYTARAAVDRSTPLAQFTAV
                     TDDAIAMPPAGDWLGWRRGYDAQGFSPLKQIDTRNVENLRLAWSWTLPAGSTEGVPVV
                     RDGTIFVEAYGDIVQALDARTGDLLWQYTHTLEQGAAPFHKRGIALHGNRLYLGTSDV
                     HVVALDVATGEVVWDTTVGDFRRREGLNGGPLVARGKVMVGTTGTGVAAKFGGPQIVG
                     LDEETGKIAWRLGTIAKPGEPGGESWNGIPFGERSGASIWTPGSYDPQTGLAYFGTGN
                     TYDTGPLRDLKPGQNNDALFTESTLAFEPRTGKLVWYYQHNRNDQF"
BASE COUNT          370 a          757 c          733 g          352 t
ORIGIN      
        1 acgttgaaga acgcggccca aataacggcc tgccacggct tgagcacgcc cgtggacacg
       61 acggtcgcga tcgaattcgc ggcgtcgtga aagccgttca tgaagtcgaa cgccagcgcg
      121 agcaccacca gcagcgcgag cgaggtcgag acttgtgcca agtccatacg ttgcgagctc
      181 ggcggccgtc agccgttgtc gagcacgacg ctttcgatga cgttcgcgac gtcttggcac
      241 ttgtccgtcg ccgactcgag tgcctcgtaa acggccttca gcttgatgac ttgacgcaca
      301 tccgtttcgt cacggaaaag ccgcgagatc gcgccgcgca tgaccttgtc ggcttcggat
      361 tcgagcttgt cgatttcgag acaaatttcg agcatcgccg gcgcctcggc catcgacgcc
      421 atgagcttca cggcggcttg cacgcgctcg gcgcagcgga gcacgagctc ggcgagctgc
      481 gtcgcctcgg gcgtcacctt ctgaatgtca tacagcacga gcgactcggc cgcgtcctgg
      541 atcagatcga gcacgtcgtc cattcgcgag atgagccggt gaatgtcgtc gcgatcgaac
      601 ggcgtcacga acgtcgtgtg catcagctgc acggtctcgt gcgtgatgcg gtcggccgaa
      661 tgctcgacgt cctcgatgag cttgatgccg gcgtcgcgac gttgctggac ttcgtagtgg
      721 cgcagcacct cggccagcgc aaccgcgccg tcgacgatga gcttcgcgtg gccgtcgaat
      781 agcgcgaaaa agcgcccctc gcggggcatg atccgggcga tgaagttcat tgttcttggt
      841 cgtccctccg cttcgagcgc gagtgtacac tgccccacgg ccttcccggg ggggttcatg
      901 ggcagagttg tcgttcttgt cgcagcgttg ctgatcggcg ccggcgccca cgctcaagaa
      961 gtcgcgctgt cgttcacggc ggcacaggcc gcgcaagggc gcgccgtcta cgacacgacc
     1021 tgcgccatgt gccacggcgc gaacttggac gacggcccgc tcggtgcgcc gctcaagggc
     1081 gacgcgttca tgcgcaagta cggcggcaaa tcggcgcgcg cgctctacga cgtgctgcgc
     1141 acgacgatgc cgacgggcaa tcccgggtcg ctgtcggcgg agacgtacgc ggcgctggcc
     1201 gctctgatct tctcgcagaa cagcatcgtc gccggcgcca cgccgctgcc cgcggatccg
     1261 cagctgctcg ccgcgatgca ggtgccggcc ggcggcttca gcttcatggc gttctcgccg
     1321 tacacggcgc gcgccgccgt cgatcgctcc acgccgctcg cgcagttcac ggccgtcacc
     1381 gacgacgcga tcgcaatgcc gcccgccggc gactggctcg gctggcgccg cggctacgac
     1441 gcgcagggct tcagcccgct caagcagatc gatacgcgaa acgtcgaaaa cctgcggctc
     1501 gcgtggagct ggacgctgcc ggccggctcc accgaaggcg tgccggtcgt gcgtgacggc
     1561 acgatcttcg tcgaagccta cggcgacatc gtgcaagcgc tcgacgcgcg cacgggcgac
     1621 ctgctctggc agtacacgca tacgctcgag caaggcgccg cgccgttcca caagcgcggc
     1681 atcgcgctgc acggcaaccg gctctatctc ggcacgtccg acgtgcatgt cgtcgcgctc
     1741 gacgtcgcga cgggcgaggt cgtctgggac acgaccgtcg gcgacttccg ccgccgcgaa
     1801 gggctgaacg gcgggccgct cgtcgcgcgc ggcaaggtca tggtcggcac gacgggcacg
     1861 ggcgtcgccg cgaagttcgg cggcccgcaa atcgtcgggc tcgacgagga gaccggcaag
     1921 atcgcgtggc ggctcggcac gatcgcgaaa ccgggcgagc ccggcggcga gagctggaac
     1981 ggcatcccgt tcggagagcg cagcggcgcg tcgatctgga cgccgggcag ctacgatccg
     2041 caaaccggcc tcgcctattt cggcacgggc aacacctacg acacgggccc gctgcgcgat
     2101 ctcaagcccg gccagaacaa cgacgcgctg ttcaccgaaa gcacgctcgc cttcgagccg
     2161 cgcacgggca agctcgtctg gtattaccag cacaaccgca acgaccagtt cg
//
LOCUS       DASSNN010000422         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_308461, whole genome shotgun sequence.
ACCESSION   DASSNN010000422 DASSNN010000000
VERSION     DASSNN010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308461"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..730)
                     /locus_tag="VFL84_07900"
     CDS             complement(179..730)
                     /locus_tag="VFL84_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET7608581.1"
                     /translation="MRTRDTRTGYGWVSIALHWLTAIVILTLWFIGSGIQSDLATGST
                     ATLRLHTSIAITAYLLLWARVIWRFKFRHPGAMPEQRGIFYEIGKYTHYVILAAVAAM
                     LLSGPLMVWSRGEAIYVFDWFTIPGPYGRNMDLYALLHGIHVWGSRVIIIGTVLHLGG
                     VYKHAAFNQDGTFGKMLVAARED"
     gene            complement(804..1007)
                     /locus_tag="VFL84_07905"
     CDS             complement(804..1007)
                     /locus_tag="VFL84_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008373402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608582.1"
                     /translation="MLTLYSPDNSRLLEVEALERSGNELLIKGKVFGAMPMTARLTPA
                     EARKGLKLLNFKLALFLLTFLVR"
     gene            complement(1007..2575)
                     /locus_tag="VFL84_07910"
     CDS             complement(1007..2575)
                     /locus_tag="VFL84_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7608583.1"
                     /translation="MSATFDIVTVGSGHNGLVAAAYLAAAGKKVLVLERNAWFGGGVV
                     TRALTVPGYLHDQHSMAHIFIQANPLLKDDELNLKAKHGLKYVFPELPMMSVFEDGTT
                     LGLYRDREKSCAEIAKFSKKDAETYRWLAAKAASYLPMIAASLYAPPAPLGPTYALMD
                     QSREGRELWRYMQMSSHDLLSSLFEHDKVRMHFARVAGENLVSPDEKATGIGVFVFVA
                     FLEAYGIGVPIGGSGKLTDALIASIREHGGEVLANADVARIKTKNGRAAGVETKDGRT
                     FDAKDGVIAALHPHDLGKMVDGVAPDVVKAASETQISEIACITLHAALNGPLKFRAGA
                     HVRAVMIELLPNKYDTIRKSFDCLRYGEFSTYPLVGLGSLTMFDPSRAPPGKATMHVW
                     DYVPYQRADGRSWDDTKHHYVKRMLAHMENFLDNVAGNVVAYHSDSPVDMERTSPSFR
                     RGDLHGIAATTYQSGAHRPIPELGQNVVPGCERLYLVGPFQHPGGGVFGAGRAAALRA
                     FEDLKLDFAKVGKR"
     gene            complement(2635..3720)
                     /locus_tag="VFL84_07915"
     CDS             complement(2635..3720)
                     /locus_tag="VFL84_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7608584.1"
                     /translation="MTETNVERGFFVALLLVVTLAFFWLIRGFLQPIFWAVALGIVVF
                     PLHARLQRRLHNRRGLAASISVVTVVIVVILPLIGLGAAVANEGAALYNRLQTGGHGI
                     GDIFTRVEQRMPQVTALIERVGVDPTRLQEQAQSAAVAVSRAIAERALSIGSGTLRGT
                     IFFFLMLYLLFFFLRDGPRILDALIRALPLGDQRERHLFGRFAEVSRATIKGTLVIGI
                     VQGTIGGIAFAALGISAPVLWGTVMALLSILPAVGTALVWLPASIFLLLTGHIFGGIA
                     LIFVGLVPIGLVDNLLRPVLVGRDTQLPDYLILLSTLGGLAAFGLAGIVIGPTIAAFF
                     LSVWQMAEQEFTPSPGQQAADPPPPQG"
BASE COUNT          700 a         1219 c         1261 g          658 t
ORIGIN      
        1 gttgttcgag caggtatcga tcgcgcgcgc gtatttcgcg atgagctgct gaatctcgag
       61 gtagtcgagc gccgtcaatt cgcctggctt cggcgcggcg gactgtgcgt gcgcgccgct
      121 cgagacggct agacctaaga gcacgagcgt agcgaaggcc gtttgcttcg acatgggtct
      181 aatcctcgcg cgccgcgacg agcatcttgc cgaacgtgcc gtcctgattg aacgcggcgt
      241 gcttgtagac gccgcccaaa tgcagcacgg tgccgatgat gatgacccgc gagccccaga
      301 cgtggatgcc gtgcagcagc gcgtagagat ccatgttcct gccgtaggga cccgggatcg
      361 tgaaccaatc gaacacgtag atcgcttcgc cgcgcgacca gaccatgagc ggccccgaga
      421 gcagcatcgc cgcgacggcc gcgagaatca catagtgcgt gtacttgccg atctcgtaga
      481 agatgccgcg ttgctcgggc atcgccccgg ggtgacggaa tttgaagcgc cagatcacgc
      541 gcgcccagag cagcaggtac gccgtgattg caatcgaggt gtgcagccta agcgtcgcgg
      601 tggagccggt cgcgaggtcc gattgaatcc cgctgccgat gaaccagagc gtcagaatca
      661 cgatggccgt gagccagtgc aaggcgatcg agacccagcc gtaacccgtc cttgtgtcgc
      721 gagtgcgcat tgacgatcga tgatacctgc tggggccgga gcgcctagac ttgatatttc
      781 tgaaggctcg cggtcgccgc gcgtcagcgc accaagaacg tcagcaagaa cagcgcgagc
      841 ttgaaattga gcagcttgag gcccttgcgc gcctcggccg gcgtcaagcg cgccgtcatc
      901 ggcatcgccc cgaacacttt gcccttgatc aggagctcgt tgccgctgcg ctcgagcgct
      961 tcgacttcta agagcctgct gttgtcgggc gagtagagcg tcagcatcag cgctttccga
     1021 ctttcgcgaa atcgagcttc aagtcctcga acgcgcgtag cgcggccgcg cggcccgcgc
     1081 cgaacacgcc gccgccggga tgctggaacg gcccgacgag ataaagccgc tcgcagcccg
     1141 gcacgacgtt ttgcccgagc tcagggatcg ggcgatgcgc gccggactga taagtcgtgg
     1201 ccgcgatgcc gtgcaggtca ccgcgcctga agctcggcga cgtgcgctcc atgtcgacgg
     1261 gactgtcgct gtggtaagcg acgacattgc ccgcgacgtt gtcgaggaag ttctccatgt
     1321 gagcgagcat gcgcttcacg taatgatgct tcgtgtcgtc ccagctccgg ccgtcggcgc
     1381 gctgatacgg cacgtagtcc cagacgtgca tcgtcgcctt gccgggcggc gcgcgcgacg
     1441 gatcgaacat cgtcaacgag cccaagccga cgagcgggta cgtcgagaat tcgccgtagc
     1501 gcaggcaatc gaacgacttg cggatcgtgt cgtacttgtt cggcaagagc tcgatcatga
     1561 cggcgcgaac gtgcgcgccg gcgcggaact tcaaggggcc gttcagcgcc gcgtgcagcg
     1621 tgatgcaggc gatctcggag atttgcgtct cgctcgccgc tttgacgacg tcgggcgcga
     1681 cgccgtcgac catcttgccc aagtcgtgcg gatgcagcgc cgcgatgacg ccgtccttgg
     1741 catcgaacgt gcggccgtct ttcgtctcga cgcccgcggc gcgcccgttc ttcgtcttga
     1801 tgcgcgcgac gtcggcgttc gcgagcactt cgccgccgtg ctcgcgaatg ctcgcgatca
     1861 acgcgtcggt gagcttgccc gatccgccga tcggcacccc gatgccgtag gcctcgagga
     1921 acgcgacgaa cacgaacacg ccgatgcccg tggctttctc gtccggcgac acgaggttct
     1981 cgccggccac gcgggcgaag tgcatgcgaa ccttgtcgtg ctcgaacaag ctcgagagga
     2041 gatcgtgcga gctcatctgc atgtaacgcc agagctcgcg gccttcgcgg ctctgatcca
     2101 tcagcgcgta tgtcggcccg agcggcgcgg gcggcgcata gagcgacgcc gcgatcatcg
     2161 gcaaatagct cgccgctttc gcggcgagcc accgataggt ctcggcgtct ttcttcgaga
     2221 acttggcgat ctcggcgcag ctcttctcgc ggtcgcggta tagcccgagc gtcgtgccgt
     2281 cctcgaacac ggacatcatc ggcagctcgg ggaacacgta tttcaggccg tgcttggcct
     2341 tgaggttcag ctcgtcgtcc ttcaggagcg ggttcgcctg gatgaagatg tgcgccatcg
     2401 agtgctggtc gtgcaggtag cccggcaccg tcagtgcgcg cgtcacgacg ccgccgccga
     2461 accaggcgtt gcgctcgagc acgagcacct tcttgccggc ggcggcgaga taggccgcgg
     2521 cgacgaggcc gttgtgtccc gagccgacgg tgacgatgtc gaaagttgcg ctcatggttt
     2581 gccgcgccgt ttgttggaca gttcgaatgc gcgtggccgt agcataaccc gtgctcagcc
     2641 ctgcggcggc ggcgggtcgg ctgcttgctg cccaggactg ggagtgaatt cctgctcggc
     2701 catttgccag acgctcaaga agaacgccgc gatcgtgggg cctatgacga tgccggcgag
     2761 accgaaggca gcgaggccgc cgagcgtcga cagcaggatc agatagtcgg gcaactgcgt
     2821 gtcgcgtccg acgagcacgg gccgcagtag gttgtcgacg agcccgatgg ggaccaagcc
     2881 gacgaaaatg agcgcgatgc cgccgaagat gtggcccgtc aggagcagga agatcgacgc
     2941 cggcagccac accagcgccg tgccgacggc cggcaaaatg ctcagtagcg ccatgacggt
     3001 gccccagagc acgggcgcgc tgatgccgag cgccgcgaac gcgatgccgc cgatcgtgcc
     3061 ctgcacgatg ccgatgacga gcgtgccttt gatcgtcgcg cgagagactt ccgcgaatcg
     3121 cccgaacagg tgtcgttcgc gctgatcgcc gagcggcagc gcgcggatca aggcgtcgag
     3181 tattcgcggc ccgtcgcgca agaagaagaa caacagatac agcatcagga agaagaagat
     3241 cgtgccgcgc aacgtgcccg agccgatcga caacgcacgc tcggcgatcg ccctgctcac
     3301 cgcgacggcg gccgattgag cctgctcctg cagccgcgtg ggatccacgc cgacgcgctc
     3361 gatcaacgcc gtcacctgcg gcattcgctg ctcgactcgc gtgaagatgt cgccgattcc
     3421 gtggccgccg gtttgcagcc ggttatagag agcagcgcct tcgttcgcga cggcggcgcc
     3481 gagccctatc aacggcagga ttacgacgat gacgaccgtc acgacgctga tgctcgcggc
     3541 gagccctcta cggttgtgca atcggcgctg gaggcgcgcg tgcagcggaa agacgacgat
     3601 gccgagcgcg acggcccaga agatcggctg caggaaaccc ctaatcagcc aaaaaaacgc
     3661 gagcgtgacc acgagaagca gcgcgacaaa aaaaccgcgt tcgacattcg tctcggtcat
     3721 cggggcacct tgaggttgac ccaaagcggg caactcaatt ctctttcgtg tgctctcgcc
     3781 accacgcgag acgttcggct atcgttttct cgtagcctgc attgccgggc tcgtacca
//
LOCUS       DASSNN010000423         3585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_816080, whole genome shotgun sequence.
ACCESSION   DASSNN010000423 DASSNN010000000
VERSION     DASSNN010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816080"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFL84_07920"
     CDS             complement(<1..198)
                     /locus_tag="VFL84_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET7608585.1"
                     /translation="MRMEFPVKAGPPARQRTECAILPVFDDRQLHGATKDFDRAARGA
                     IAKLIRAGDSPARLGSCTLVHR"
     gene            353..1444
                     /gene="lptF"
                     /locus_tag="VFL84_07925"
     CDS             353..1444
                     /gene="lptF"
                     /locus_tag="VFL84_07925"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HET7608586.1"
                     /translation="MNLIGRYIFREAFGSWLVVMAVLFVIFMTNQLADILGDAAGNRL
                     PRDAVLAIFGLTALRYLTFLTPIGLFLGVTLALARLNRDGEMAALFACGISAARMLVP
                     IGAFTLLLALGLSWLSLEAMPAANMRIEQIRFSAKQNAELTAIEPGKFTTPDSGDTVL
                     YAREVVADELRDVFLESQRGDRVSVVLAERGRRIVDPSTGELSFALYNGRLYKGVPGE
                     SKFLVIEFDEQLVPIRPDNEDEFVEAVAAKPTRDLLRSPTLADRAELHWRLSFPLSLF
                     VLALVAVPLTRTSPREGRYARLGIALLVYVIYFDLLLIARVWVERGIITDAVGMWWVH
                     AVVALLGLLMLARESGWFVRARVVVPQAA"
     gene            1441..2511
                     /gene="lptG"
                     /locus_tag="VFL84_07930"
     CDS             1441..2511
                     /gene="lptG"
                     /locus_tag="VFL84_07930"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HET7608587.1"
                     /translation="MTLLRTYIVLAVLRGVATVMVVLLAVTCVIEFVGQLNDVGTGDY
                     GLQTALLYIGLRMPGRIFTTLPIGALIGALLSLGNLAVHRELVVMRASGVSSLQLLSA
                     VGLGGLVLAVLMVLLGESVAPSLGAYASEMRTRAMHEEIAVGDGQATWLREGDRIVAL
                     RRQAGDLGYGGGVLLFELGPDQSLREVASADSADLDAANRWVLSNYAETSFEPGGVAV
                     RTSRESSEAHGINPDLLELSVVRADLLDTPSLVRYISYLRENGLDAHRYLVAYWSRLA
                     SVFSVVVMTVLALPFVFGGLRSAGAGARLLVGLIMGLTYYVTVQVLESGGEVYRLDPL
                     VIAWAPSVLLVLATTIAFARIR"
     gene            complement(2508..2966)
                     /locus_tag="VFL84_07935"
     CDS             complement(2508..2966)
                     /locus_tag="VFL84_07935"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET7608588.1"
                     /translation="MKSTSETASKLPLEPAPVWRRLAAFCYDLLLIVALAASFTALVV
                     AVRLGAGVPPGTWWFSLSLLGLIAAFFCGFWAHGGQTLGMRAWRIRVVRDDGGALTLA
                     RAAARFVCGVVAAAPAGLGLWWSFLDERKRGWHDRWTGTRVVRAIAASGR"
     gene            complement(2989..3267)
                     /locus_tag="VFL84_07940"
     CDS             complement(2989..3267)
                     /locus_tag="VFL84_07940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7608589.1"
                     /translation="MTLAIRHELTTRRFAADVGGKTAYITYRERDGGVLDLDHTYVPR
                     EHRGSGIASQLTARALEYARENGYRIVPSCPFVAAYLERHPEDRDLRA"
     gene            complement(3275..>3585)
                     /locus_tag="VFL84_07945"
     CDS             complement(3275..>3585)
                     /locus_tag="VFL84_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608590.1"
                     /translation="SGARYDFRVYVWETKFKAIPGVYVVAARTIEPGQPPQYQPLFVG
                     ETADLATVFRDHSRDECFQMYYGNVVGVLKESDDARRTSILADLIAGLAPPCNAESAP
                     "
BASE COUNT          527 a         1159 c         1210 g          689 t
ORIGIN      
        1 tcggtgaacg agcgtgcagg agccgagacg tgctggactg tcgccggcgc gaatcaactt
       61 tgcgatggcg ccgcgtgcgg ctcgatcgaa gtctttggtc gcgccgtgca gctgacggtc
      121 atcgaacacc ggcaggattg cgcactcggt tcgctggcgc gctggtggac ccgccttgac
      181 cgggaactcc atcctcattt ctccttcgtc cgctccgctc tttttggtga acgctgcgac
      241 tcgcgagcgt cgcggcgacg gcgtcgcgct gtgctatcgt gttgtaatag cgtggaaaat
      301 cgcagtgtag agcattactt tcgctgaagg tactgggagc cggaccgcaa ccgtgaatct
      361 gatcggccgc tatatcttcc gtgaagcttt cggttcgtgg ttggtggtca tggcggtcct
      421 gttcgtgatc ttcatgacga accagctcgc cgatattttg ggcgacgccg caggaaaccg
      481 actgccgcgc gacgcggtgc tcgcgatctt cggcctcacg gcgctgcgat acttgacgtt
      541 tctgacgccg atcggcttgt tcttgggtgt cacgctcgca cttgcgcgcc tcaatcgcga
      601 tggcgaaatg gccgcgttgt tcgcgtgcgg aatcagcgcg gcgcgcatgc tcgttccgat
      661 cggcgcgttc acgttgctgc tggcgctcgg gctgagctgg ctctcgctcg aggcaatgcc
      721 ggccgcgaac atgcgcatcg agcagattcg cttcagcgcg aagcagaacg cggagctgac
      781 ggcgatcgag cccggaaagt tcacgacgcc cgactcgggc gacaccgtgc tctatgcacg
      841 cgaggtcgtc gccgacgagc tgcgcgacgt gtttctggag tcgcagcgcg gcgatcgagt
      901 cagcgtcgtg cttgcggagc gcgggcgccg catcgtcgat ccgtcgaccg gcgagctatc
      961 gttcgcgctc tacaacgggc ggctctacaa gggcgttccc ggcgaaagca agttcctcgt
     1021 gatcgagttc gacgagcagc tcgtgccgat ccgacccgac aatgaagacg agttcgtcga
     1081 ggccgtggcc gcgaagccga cgcgcgacct cttgcgctcg ccgacgctcg ccgatcgcgc
     1141 ggagcttcat tggcgcctgt ctttcccgct gtcgttattc gtgctggcgc tggtcgccgt
     1201 gccgctgact cgaacctcgc cgcgcgaagg ccgttatgcc cggctcggga tcgcgttgct
     1261 cgtctacgtc atctacttcg atctgctgtt gattgcccgc gtctgggtcg agcgcggcat
     1321 catcacggac gccgtcggaa tgtggtgggt gcacgctgtc gtcgcgctgc ttgggctgtt
     1381 gatgctcgcg cgcgagagcg ggtggttcgt tcgagcgcgc gtcgtcgtgc cgcaagccgc
     1441 atgactctgc tgcgtaccta tatcgtgttg gccgtgctgc gcggcgtcgc gaccgtgatg
     1501 gtcgtgctgc tcgccgtgac gtgtgtcatc gagttcgtgg gccagctcaa cgacgtcggc
     1561 acgggcgact acggcctgca gacggcgttg ctctacatcg gcttgcgcat gcctgggcgg
     1621 attttcacga cgttgccgat cggcgcattg atcggcgcgc tgctttcgct cggcaatctg
     1681 gccgtgcacc gcgagctcgt cgtcatgcgg gccagcggcg tttcgtcgct gcagttgctc
     1741 tctgccgtcg ggctcggcgg cttggtcctc gcggtgctga tggtgcttct cggtgagtcc
     1801 gtggctccgt cgctcggcgc ttacgcaagc gagatgcgca cgcgcgcgat gcacgaagag
     1861 atcgcggtcg gcgacggaca ggcgacctgg ctgcgcgaag gcgatcgtat cgtcgcgttg
     1921 cggcgccagg cgggcgatct cggctacggc ggcggcgtgc tgctgttcga gctcggtccg
     1981 gaccagtcgc tcagggaggt cgcgagcgcg gattcggccg atctcgatgc ggctaatcgc
     2041 tgggtgctct cgaattatgc cgagacttcg ttcgagccgg gcggcgtcgc cgtgcgcacg
     2101 agccgcgaat cgagcgaagc gcacggcatc aacccggatt tgctggagct gtcggtcgtg
     2161 cgcgcggacc tactcgatac cccgtcgctc gtccggtaca tctcttatct gcgcgagaac
     2221 ggcttggacg cgcaccgcta tctcgtcgcg tactggtcgc ggctcgcgag cgttttctcg
     2281 gtcgtcgtca tgaccgtgct cgcgttgcca ttcgtgttcg gcggcttgcg ctcggcgggc
     2341 gcaggcgctc ggctcctcgt cggtctcatc atgggcctca cgtattacgt cacggtccaa
     2401 gtgttggaga gcggcggcga ggtgtatcga ctggatccgc tcgtcatcgc gtgggcgccg
     2461 tctgtgctgc tcgtgctcgc gacgactatc gcgttcgcac gcatccgcta gcgtccgctc
     2521 gccgcgattg cgcgcaccac gcgcgtgccc gtccagcgat cgtgccaacc gcgcttgcgc
     2581 tcgtcgagga agctccacca caagccgagc ccggcgggcg ccgcggcgac gacgccgcag
     2641 acgaatctcg cggcggccct cgccaacgtc agcgcgccgc cgtcgtcacg gacgacgcgg
     2701 atccgccacg cgcgcatgcc gagcgtttgg cccccgtgcg cccaaaagcc gcaaaagaac
     2761 gcggcgatga gccccagcaa gctcagcgaa aaccaccacg tcccgggcgg cacccccgct
     2821 cccaggcgca cggccaccac gagcgccgtg aacgaggccg cgagcgcgac gatcagcagc
     2881 aggtcatagc aaaacgccgc gagccggcgc cacacgggag cgggctcgag cggcagcttc
     2941 gaagcagttt ccgaagtgct cttcaacgga gatgccaagc gcgactgctc aggcgcgaag
     3001 gtcgcgatcc tcgggatgac gctcgaggta ggcggccacg aacggacaac tcggcacgat
     3061 tcggtaaccg ttctcacgcg cgtactcgag cgcgcgcgcc gtcagctgcg acgcgatgcc
     3121 gctgccgcgg tgctcgcgcg gaacgtacgt gtggtcgagg tcgaggacgc cgccgtcgcg
     3181 ctcgcggtac gtgatgtagg cggtcttgcc gccgacgtcc gcggcgaacc gccgcgtcgt
     3241 tagttcgtga cgaatggcga gcgtcatgtc gctctcaggg cgcgctttcg gcattgcacg
     3301 gcggggcgag gccggcgatc aggtcggcga gaatcgacgt gcgccgcgcg tcgtcgctct
     3361 ctttgagcac tccgaccaca ttgccgtaat acatctgaaa gcactcgtcg cgtgagtgat
     3421 cgcggaacac cgtcgccaag tcggccgtct cgccgacgaa caacggttgg tattgcggag
     3481 gttggcccgg ctcgatcgtg cgcgccgcga cgacgtaaac gccggggatc gctttgaact
     3541 tcgtctccca tacgtagacg cggaagtcgt aacgcgctcc cgaac
//
LOCUS       DASSNN010000424         3627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_204585, whole genome shotgun sequence.
ACCESSION   DASSNN010000424 DASSNN010000000
VERSION     DASSNN010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204585"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /gene="prpB"
                     /locus_tag="VFL84_07950"
     CDS             complement(<1..199)
                     /gene="prpB"
                     /locus_tag="VFL84_07950"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014292460.1"
                     /note="catalyzes the formation of pyruvate and succinate
                     from 2-methylisocitrate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HET7608591.1"
                     /translation="MSAGKRFRRLVARKPPLQVPGAVNAYCALLAEKAGFEALYLSGA
                     GVANASFGLPDLGITTLNDVLE"
     gene            complement(196..1635)
                     /locus_tag="VFL84_07955"
     CDS             complement(196..1635)
                     /locus_tag="VFL84_07955"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HET7608592.1"
                     /translation="MSSHDIRSAERPAPDKLLTELADYVLRDAAESEEARNIARYCLM
                     DTLGCGILALGFPACTKLLGPVVPGAELAGGARVPGTRYELEPVAAAFNIGAMIRWLD
                     FNDTWLAAEWGHPSDNLGAILAVSDYLARQGTRLKMADVVTAMIQAHEIQGVLALNHS
                     FNRVGLDHVLLVRVASTAVATRLFGGTREQIVNAVSNAWLDGGALRTYRHAPNTGSRK
                     SWAAGDATSRGVRHALLARAGEMGYPSALTAKGWGFQDVLFKGKTIALERRLGTYVME
                     NVLFKISFPAEFHAQTAVEAALKLHAIVKPRIADIARVTIETQEAGMRIIDKTGPLAN
                     PADRDHCLQYMVAVPLLLGRLTAEDYEDEVARDPRIDALRAKMVVTENAEFSRDYLDA
                     DKRAIGNAVQVWFADGTRTERVAIDYPIGHRRRRAEGIPQLVAKFERNLSTLFGAQQR
                     NAISAACADQDRFEKLPVQDFMALWVLPQ"
     gene            1677..2507
                     /gene="rlmJ"
                     /locus_tag="VFL84_07960"
     CDS             1677..2507
                     /gene="rlmJ"
                     /locus_tag="VFL84_07960"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HET7608593.1"
                     /translation="MLSYLHGYHAGNHADVLKHTVLTALLARLVAKDKPLRYIDTHAG
                     AGGYDLRAPPAQRNREHEGGIGKIWDADDAPPAVARLLTLAHRFNGGGPLKRYPGSPW
                     LARESLRPIDDLFLFELHPAEHRALKNACAGDRRVKVQRENGLTECIGLVPPPSKRAL
                     LLVDPSYELRDEHRDVVDALAKAHKRFTTGVFAIWYPVIERRWVERYERALRATGIAA
                     ISTYELCVASERRDGGLVGSGVFVVNAPWQLDDELGAALPWLAQRLGAEREASHRIVV
                     "
     gene            complement(2534..>3627)
                     /locus_tag="VFL84_07965"
     CDS             complement(2534..>3627)
                     /locus_tag="VFL84_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HET7608594.1"
                     /translation="RGAAAYVGLFSMLASLVSWSFSLIFSALLAREVAQRVRGSDYRA
                     VGAAAYLGVGSVWALGMSSSAALIMATPASLPASIGSITGTIPLSQTIGLWQSLLIAA
                     VLIVVSMVVSYYSAPSAAHAKSMSDMGVTYAPATSDLGKRLTPGEWLEYSPLLTIVIC
                     VLGFGYLAREVMTSGAGVLLDLNHYLFLFLMAGLLLHWRPKSFVQAIAASVTPVGGVL
                     IQYPMYAGILEMLTKSGLAEDISQFFVRFSTEHTFPVLVGIYSAFLGLFIPSAGGKWL
                     LEAPYLLGAATEHEVHLGWVVQTYNATEALANLIHPFWMLPLVGILGLKARDIVGYSM
                     LQFVVHVPLVLFLVWLLNYTLTYVPPVLP"
BASE COUNT          627 a         1239 c         1218 g          543 t
ORIGIN      
        1 cctcgagcac gtcgttgagc gtcgtgatcc ccaaatcggg caatccgaac gatgcattgg
       61 cgacaccggc gccggataaa tacagcgctt cgaagccggc cttctcggct agcaacgcgc
      121 aatacgcgtt gacggcgccc ggaacttgca gcggcggctt gcgcgcgacg aggcggcgaa
      181 agcgcttgcc cgcgctcatt gcggcagcac ccacaacgcc atgaagtcct ggacaggcag
      241 cttctcgaac cggtcttggt ccgcgcacgc cgccgagatc gcattgcgct gctgcgcgcc
      301 gaacaacgtc gagagattgc gctcgaactt cgcgacgagc tgcgggatgc cttcggcacg
      361 cctgcgccga tggccgatcg gatagtcgat ggccacgcgc tccgtgcgcg tgccatccgc
      421 gaaccacact tgcacggcgt taccgatcgc gcgcttgtcg gcgtcgagat agtcgcggct
      481 gaattccgcg ttctccgtga cgaccatctt ggcgcgcaac gcatcgatgc gcggatcgcg
      541 cgcgacctcg tcctcgtagt cttcggcggt caaacgcccg agcagcagcg gcacggcgac
      601 catgtactgc aggcagtgat cgcgatcggc cggattcgcg agcgggccgg tcttgtcgat
      661 gatgcgcatg ccggcctctt gtgtctcgat cgtgacgcgc gcgatgtcgg cgatgcgcgg
      721 cttgacgatc gcgtgcagct tcagcgcagc ctcgactgcc gtctgcgcgt ggaactcggc
      781 cgggaacgat atcttgaaca gcacgttctc catgacgtac gtgccgagcc ggcgctcgag
      841 cgcgatcgtc ttgcctttga acagcacgtc ctgaaagccc cagccctttg cggtcaacgc
      901 cgacgggtaa cccatctctc cggcgcgggc gaggagcgca tgccgcacgc cgcggctcgt
      961 cgcatcgccg gcggcccagc tcttgcgcga gccggtgttc ggcgcgtgcc gataggtgcg
     1021 cagcgcgccg ccgtcgagcc aagcgttcga gacggcgttg acgatttgct cgcgcgtgcc
     1081 gccgaaaagc cgcgtcgcga cggccgtgct cgcgacacgc acgagcagca cgtggtcgag
     1141 cccgacgcga ttgaagctgt ggttcagcgc aagcacgcct tggatctcgt gcgcctgaat
     1201 catcgccgtc acgacgtcgg ccatcttgag ccgcgtgccc tgccgcgcaa gatagtcgga
     1261 gacggcgaga atcgcgccga ggttgtccga cggatggccc cactcggccg cgagccacgt
     1321 gtcgttgaag tcgagccagc ggatcatcgc gccgatgttg aacgccgcgg cgacgggctc
     1381 gagctcgtag cgagtgcccg gcacgcgcgc gccgcccgcg agctcggcgc cgggcacgac
     1441 ggggccgagc agcttcgtgc aggcaggaaa accgagcgcg aggatgccgc agccgagcgt
     1501 gtccatgagg caatagcgcg cgatgttgcg cgcctcttcg ctctcggccg cgtcgcgcag
     1561 cacgtaatcg gcgagctccg tgagtagctt gtccggtgcc gggcgctccg cgctgcggat
     1621 gtcgtgggag ctcatggctc gttgtctttc attcgacgcg gcaatatagc acctgcatgc
     1681 tgagctacct gcacggttat cacgccggca atcatgccga cgtcctcaag cacacggttt
     1741 tgaccgcgtt gctcgcgcgt ctcgtcgcca aggacaagcc gctgcgctac atcgacaccc
     1801 acgccggcgc gggcggctac gatctgcgcg cgccgcccgc ccagcgcaac cgcgagcacg
     1861 agggcggcat cggcaagatc tgggacgcgg acgacgcgcc gcccgccgtc gcgcgcctcc
     1921 tcacgctcgc gcatcgtttc aacggcggcg ggccgctgaa gcgctatccc ggctcgccgt
     1981 ggctcgctcg cgagtcgctg cgcccgatcg acgacctgtt cttgttcgag ctccacccgg
     2041 ccgagcaccg cgcgttgaag aatgcctgcg ccggcgaccg ccgcgtgaag gtgcagcgcg
     2101 agaacggcct cacggagtgc atcggtctcg tcccgccgcc gtcgaagcgc gcgttgctgc
     2161 tcgtcgaccc gtcgtacgag ctgcgcgacg agcatcgcga cgtcgtcgac gcgctcgcga
     2221 aagcgcacaa gcggttcacg accggcgtgt tcgccatctg gtatccggtc atcgagcggc
     2281 gctgggtcga gcgctacgag cgcgcgctgc gagcaacggg catcgccgcg atctcgacgt
     2341 acgagctctg cgtcgcgagc gagcggcgcg acggcgggct cgtcggcagc ggcgtgttcg
     2401 tcgtcaacgc gccgtggcag ctcgatgacg agctcggcgc cgcgctgccc tggctcgcgc
     2461 agcgcctcgg cgccgagcgc gaagcgagcc accgcatcgt cgtttagccg gcgcggtcac
     2521 gagccgaccg ccgtcacggc aggacgggcg gaacgtacgt cagcgtgtag ttgagcagcc
     2581 agacgaggaa cagcacgagc ggcacgtgca cgacgaactg cagcatcgaa tagccgacga
     2641 tgtcgcgcgc cttgagcccg aggatgccga cgagcggcag catccagaac gggtggatca
     2701 gattcgcgag cgcctcggtc gcgttgtagg tctgcacgac ccaacccaag tgcacctcgt
     2761 gctcggtcgc ggcgccgagc aggtacggcg cctcgagcag ccacttgccg cccgccgacg
     2821 gaatgaacag cccgaggaac gccgagtaga tgccgaccag gaccggaaac gtgtgctcgg
     2881 tcgagaagcg gacgaagaac tgcgagatgt cctccgcgag acccgacttg gtcagcatct
     2941 cgaggatccc ggcatacatc gggtactgga tcagcacgcc gccgacgggc gtcacggatg
     3001 ccgcgatcgc ctgcacgaac gacttcggcc gccaatgcag gagcagcccg gccatcagga
     3061 acaagaacaa gtagtgattg aggtcgagca ggacgccggc gccgctcgtc atgacctcgc
     3121 gagcgaggta gccgaagccg agcacgcaaa tcacgatcgt caagagcggg ctgtactcga
     3181 gccattcgcc gggcgtcagc cgtttcccga ggtcgctcgt cgcgggcgcg tacgtcacgc
     3241 ccatgtcgct catcgacttc gcgtgcgcgg cgctcggcgc cgagtagtac gacacgacca
     3301 tcgagacgac gatcagcacg gcggcgatga gaagactctg ccaaaggccg atcgtttggc
     3361 tcaacggaat cgtgcccgtg atgctgccga tcgacgcggg caaagacgcc ggcgtggcca
     3421 tgatcaacgc ggccgacgac gacatgccga gcgcccagac gctgccgacg ccgagatacg
     3481 cggccgcgcc gaccgcacga tagtccgagc cgcgaacgcg ttgcgcgacc tcgcgcgcga
     3541 gcagcgcgct gaagatcagg ctgaagctcc acgacacgag cgacgcgagc atcgagaaca
     3601 gcccgacgta agcggccgcg ccgcggc
//
LOCUS       DASSNN010000425         4000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1206317, whole genome shotgun sequence.
ACCESSION   DASSNN010000425 DASSNN010000000
VERSION     DASSNN010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206317"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2166)
                     /locus_tag="VFL84_07970"
     CDS             complement(<1..2166)
                     /locus_tag="VFL84_07970"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HET7608595.1"
                     /translation="MAEYVAHGGIKVASTLYDLVRDEIAPGSGVKPDAVWTLLDSIVR
                     TLGPRNRALLEKRDALQAKIDAHLAAQRGKPLDPKAMAEFLREIGYLVPNGPPFTVTT
                     ANVDPEIATLAGPQLVVPVDNARYALNAANARWGSLYDALYGTNVIPEDNGAERGRGY
                     NAVRGERVMATASEFLDQAVPLASAKWIDVMGVEVSGTRLACRLRDDRTTTGLADAAQ
                     LVGFRRSGNALTNVLLRNHGLHIDIAIDKSHPIGKQHPAGIKDIVLESALTTIMDCED
                     SVAAVDAADKAVVYRNWCGIMRGTLTASVAKGGGTIERRLNADREYTALDGTTLTLPG
                     RSLLLVRHVGSHMVTDAVTDSAGNPTPETFLDCVLTTLAALHDIKGKNALRNSRTGSM
                     YIVKPKHHGPEEVALSVELFATVERAFGLPANTLKIGIMDEERRTTVNLKECIRAAKD
                     RIIFINTGFLDRTGDELHTNMEAGPVIPKPEIKGARWIAAYEDWNVRVGLEVGLPGHG
                     QIGKGMWAMPDEMRAMLDTKQGHPKAGATTAWVPSPTAATLHAIHYHQIDVAARQREI
                     ARQGGASRELLEGILTPPLLDRKLTAAEIQRELDNNAQGILGYVVRWVEQGVGCSKVP
                     DINDVGLMEDRATLRISSQHIANWLRHGIVDREQVRATLERMAEVVDRQNSGDRHYRK
                     MAPKFEESIAFQAALDLVFNGLKEPNGYTEHTLTRRRREF"
     gene            2504..>4000
                     /locus_tag="VFL84_07975"
     CDS             2504..>4000
                     /locus_tag="VFL84_07975"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET7608596.1"
                     /translation="MKAANVLCIRPRVRSLSKAITEVLRGAPVLAVSSGAILGAAFAV
                     APPSRVAAQAQPPQQLEEITVTGSRIQRQDFTANSPITTIDEASFQNTSTIGVEKVLN
                     ELPQFVPALSQFTTTDVQQTANNTIGARTLSLRGLGANRNLVLIDGKRAMPINPTMVI
                     DTNSIPRSAIARVEVITGGASAVYGADAVGGVVNFILKDDFEGASIDVRYGDTQHGGN
                     EEVSFSGLLGANMADGRGNVMLGLERSTQTKVRQQDRDWRVEDMANPATPATAFGWGS
                     DTWISSDGSNTPIIGPNALNALGVSLNNALFDDFPDQNVVNAMFNNGQNGSLDCAVPN
                     IAPPGAPPVFNPYSGGVCPQAPGAEYLGVPNTARFLLNRPSGTLYTGLMNNQGGSRSD
                     LYQGPYSGDHYGNFQGLPFRVVQPDGQIKENNFWQWASGPLERLSAFGKGHFDISDNV
                     RVTGTASYTRTKAETSLGLTADLITFWGAAVPFGLDEYTGNPAAGIPLP"
BASE COUNT          658 a         1332 c         1261 g          749 t
ORIGIN      
        1 gaactcgcgg cgccgccgcg tgagcgtgtg ctcggtgtag ccgttgggct ccttcaatcc
       61 attgaacacg aggtcgagcg ccgcttgaaa cgcgatgctc tcctcgaact tcggcgccat
      121 cttgcgatag tggcggtcgc ccgagttttg ccggtcgacg acttcggcca tgcgctcgag
      181 cgtcgcgcgc acttgctcgc ggtcgacgat gccgtgacgg agccagttcg cgatgtgctg
      241 cgacgagatg cgcagcgtcg cgcgatcctc catgagcccg acgtcgttga tgtcgggcac
      301 cttcgagcag ccgacgcctt gctcgaccca gcgcacgacg tagcccaaga tgccttgcgc
      361 gttgttgtcg agctcgcgct gaatctcggc ggccgtcagc tttcgatcga gcagcggcgg
      421 cgtcaggatt ccctcgagca gctcgcgact agcgccgcct tgccgcgcga tctctcgttg
      481 gcgcgcagcg acgtcgatct ggtgatagtg aatcgcgtgc aacgtcgcgg ccgtcggcga
      541 cgggacccag gccgtcgtcg cgccggcctt cggatggcct tgcttcgtgt cgagcatcgc
      601 gcgcatctcg tccggcatcg cccacatgcc tttgccgatc tgaccgtgac cgggtagccc
      661 gacctcgagc ccgacccgca cgttccaatc ttcgtaggcg gcgatccagc gcgcgccttt
      721 gatctcgggc ttcgggatca cggggcccgc ttccatgttt gtgtgcagct cgtcgccggt
      781 gcgatcgagg aagcccgtgt tgatgaagat gattcggtcc ttcgccgcgc ggatgcactc
      841 cttcaagttg acggtcgtgc gccgctcctc gtccatgatg ccgatcttca gcgtgttcgc
      901 aggcaggccg aacgcgcgct cgacggtcgc gaacagctcc acggacagcg cgacttcctc
      961 ggggccgtgg tgcttcggct tcacgatgta catgctgccg gtacggctgt tgcgcagcgc
     1021 gttcttgccc ttgatgtcgt gcaatgccgc gagcgtcgtc agcacgcagt cgagaaacgt
     1081 ttcaggcgta gggttgccgg cgctatccgt gacggcgtcg gtcaccatgt gcgagccgac
     1141 gtggcgcacg agcagcaagc tgcggcccgg cagcgtcaac gtcgtgccgt cgagcgccgt
     1201 gtattctcga tccgcgttca gacgtcgctc gatcgtgccg ccgcccttcg cgaccgacgc
     1261 cgtcagcgtg ccgcgcatga tgccgcacca gttgcggtag acgaccgcct tgtcggccgc
     1321 gtcgacggcg gcgaccgagt cctcgcaatc catgatcgtc gtcagcgcgc tctcgagcac
     1381 gatgtccttg atgccggccg gatgctgctt gccgatcgga tgcgacttgt cgatcgcgat
     1441 gtcgatgtgc agcccgtgat tgcgcagcag cacgttcgtc agcgcgttgc cgctgcggcg
     1501 aaagccgacg agctgcgccg cgtcggccaa gcccgtcgtc gtgcgatcgt cgcgcagccg
     1561 gcaggcgagg cgcgtgcccg aaacctcgac gcccatgacg tcgatccact tcgcgctcgc
     1621 gagcgggacg gcttggtcga ggaactcgct tgccgtcgcc atcacgcgct cgccgcgcac
     1681 ggcgttgtag ccgcggccgc gctcggcgcc gttgtcctcg gggatcacgt tcgtgccgta
     1741 gagcgcgtcg tagagactgc cccagcgcgc gttcgcggcg ttcaacgcat accgcgcgtt
     1801 gtcgacggga accacgagct gcgggcccgc gagcgtcgca atctcgggat cgacgttcgc
     1861 ggtcgtcacc gtaaacggcg gcccgttcgg cacgagatag ccgatctcgc gcaagaactc
     1921 ggccatcgct ttcgggtcga gcggcttgcc gcgctgcgcc gcgaggtgcg cgtcgatctt
     1981 cgcttgcagc gcgtcgcgct tctcgagcag cgcacggttg cgcgggccga gcgtgcgcac
     2041 gatcgagtcg agcagcgtcc aaacggcatc gggctttacg cccgagccgg gcgcgatctc
     2101 gtcgcgtacg agatcgtaca gagtgcttgc gaccttgatt ccaccgtgcg cgacgtactc
     2161 agccactgaa gttttgctcc tcgagtttga tcgctagcgc cgacgcgaaa accgtcggcg
     2221 tccaggcagg ataacggcct cggccggatc cgcaacgctt cgccttgacg agcgccgggt
     2281 cgtttcccgg cccgaattct aatattagtg tgtggtcatt cgttttctcc aagtcgtggc
     2341 gccggcgcga ctccgactct cccagacgtg aaagcgattg caccgtagtg ccaatgtgtt
     2401 agtctcgaac ccgcaacaat cgtcaacacc gggttcccgg gatgacattg cagggatgcc
     2461 cggtgcgtgg ttcaaggcat gccgtcacca cagggggaga acaatgaagg ctgcgaacgt
     2521 tctgtgtatc cgcccgcgcg tccgttcact ctcgaaagcc atcacggaag tgctacgcgg
     2581 cgctcccgtg ctcgcggtct cgagtggagc gattctcggc gccgcgtttg cggtcgcgcc
     2641 gcccagccgc gtcgcggctc aggcacaacc gccgcaacag ctcgaggaaa tcaccgtcac
     2701 gggctcgcgc attcagcgcc aagatttcac ggccaacagc ccgatcacga cgatcgacga
     2761 ggcttcgttc cagaacacga gcacgatcgg cgtcgaaaaa gttttgaacg agttgccgca
     2821 attcgttccg gctctctcgc agttcacgac gacggacgtc cagcagacgg cgaacaacac
     2881 gatcggcgcg cgcacgttga gcctgcgcgg cctcggcgcg aaccgcaacc tcgtgctgat
     2941 cgacggcaag cgcgccatgc cgatcaatcc gacgatggtc atcgatacga actcgattcc
     3001 acgctccgcg atcgcgcgcg tcgaggtcat caccggaggc gcttcggccg tgtacggcgc
     3061 ggatgccgtc ggcggcgtcg tcaacttcat tctcaaggac gacttcgaag gcgccagcat
     3121 cgacgtgcgc tacggcgaca cgcagcacgg cggcaacgaa gaagtcagtt tctcggggct
     3181 ccttggcgcg aacatggccg acggccgggg caacgtcatg ctcggcctcg agcgctcgac
     3241 gcaaacgaag gttcgccagc aggatcgcga ttggcgcgtc gaggacatgg cgaatcccgc
     3301 gacgcccgca accgcgttcg gctggggcag cgatacgtgg atctcgagcg acggctcgaa
     3361 cacgcccatc atcggcccca atgccttgaa tgccttgggt gtgagcctca acaacgcgct
     3421 gttcgacgac ttccccgacc agaacgtcgt caacgcgatg ttcaacaacg gccagaacgg
     3481 ctcgttggat tgcgcggtac cgaacatcgc gcctcccggc gctcctcccg tctttaatcc
     3541 ctattccgga ggcgtatgtc cccaggctcc gggcgccgaa tatcttggag tcccgaacac
     3601 tgcgcgcttc ttgctcaatc gaccgtcggg caccttgtat acgggtctca tgaacaatca
     3661 gggtgggtcc cgatcggatc tctaccaagg tccgtactcg ggcgatcatt acgggaattt
     3721 ccagggactg ccgtttcgcg tcgtccagcc ggacggccaa atcaaggaga acaatttctg
     3781 gcagtgggca tcgggcccgc tcgagcggtt atcggccttt ggcaaagggc atttcgacat
     3841 ctccgataac gtccgcgtca ccggtactgc aagctacacg cgcacgaagg ccgagacgag
     3901 cctcggcctc acggccgacc tcattacgtt ctggggcgcc gctgttccgt tcggcctgga
     3961 cgagtacaca ggcaatcccg ccgcgggcat tccgttgccc
//
LOCUS       DASSNN010000426         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1822737, whole genome shotgun sequence.
ACCESSION   DASSNN010000426 DASSNN010000000
VERSION     DASSNN010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822737"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1819)
                     /locus_tag="VFL84_07980"
     CDS             complement(20..1819)
                     /locus_tag="VFL84_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET7608597.1"
                     /translation="MSQQDEYQWLFRRSPALLVSLNEDGFFVDASDAWLRRFGYAREE
                     ITNMRPQDLASPEGAKRIVEEYLPVLRRTGKLAGVPIDLVTRNNERVDCLANAIVERS
                     DAGDYLRTIAVYTEVGQQARLEQHYRELYRATPAMLHTVDKHGRITNVSDRWLESLGY
                     RREEVLGRVITEFMGKELQARLAGGRLEDVIATGELEDEPRTYVAKSGDVLEVEVSAT
                     GDRDSTGAVVALLVASKDVTARNRAERQLRAAFEENARLRDELERERDYLREEVQVSM
                     NYGRIIGESPALKQMLARLEAVAETQASVLIEGESGVGKELVAHVIHARSPRAKGPLV
                     KVNCASIPHELFESEFFGHVRGSFTGAHRDRVGRFQLADGGTIFLDEVGEIPLDLQSK
                     LLRVLQESEYERVGDDRTHSVDVRVIAATNRDLEKAVADGSFREDLFYRLSVFPIEVP
                     PLRARGDDVIQIASHFLERTCQDFGHRPLTLSKQQAALLKRYDWPGNVRELKNVIERA
                     VILSRGKVLRLDLAMSDILNPPAASSPEAKHVESTLLTELEMRELERKNTLLALEMAD
                     WRVSGPNGAAKLLGIKPTTLTDRMKKFELRKPS"
BASE COUNT          335 a          683 c          670 g          397 t
ORIGIN      
        1 gtgcggaact gttccgcggc tatgacggct tccgcagctc gaactttttc atgcggtccg
       61 tcagcgtcgt cggcttgatc ccgagaagct tggcggcgcc gttcgggccc gagacgcgcc
      121 agtccgccat ctcgagcgcg agcaacgtgt tcttgcgctc gagctcacgc atctcgagct
      181 cggtgagcag cgtgctctca acgtgcttcg cctcgggcga agaggcggcg ggcgggttca
      241 ggatgtcgct catcgcgagg tcgagccgca gcaccttgcc gcgcgacaag atcacggcgc
      301 gctcgatgac gttcttgagc tcacggacgt ttcccggcca gtcgtaacgc ttcagcagcg
      361 cggcttgttg cttcgagagc gtcaaggggc gatggccgaa gtcttgacag gttcgttcga
      421 gaaagtggct cgcgatctgg atcacgtcgt cgccgcgcgc gcgcagcggc ggcacttcga
      481 tcgggaacac gctcaagcga tagaacaggt cctcgcgaaa gctgccgtcg gcgacggctt
      541 tctcgagatc ccgattcgtc gcggcgatga ctcgaacgtc gacgctgtga gtgcggtcgt
      601 cgccgacgcg ctcgtactcg ctctcctgca gcacgcgcag cagcttgctc tgcagatcga
      661 gcggaatctc gccgacctcg tcgaggaaga tcgtgcctcc gtctgcgagc tggaagcggc
      721 ccacgcgatc gcgatgcgcg ccggtgaacg agccgcggac gtggccgaag aactcgctct
      781 cgaaaagctc gtgcggaatc gacgcgcagt tgaccttcac gagcgggcct ttcgcgcgcg
      841 ggctgcgcgc gtggatcacg tgcgccacga gctccttgcc gacgccggac tctccttcga
      901 tcagcacgct cgcctgagtc tccgcgaccg cctcgagccg cgcgagcatc tgcttcagcg
      961 ccgggctctc gccgatgatg cgcccgtagt tcatcgagac ctgcacttct tcgcgcaggt
     1021 agtcgcgctc gcgctcgagc tcgtcgcgga ggcgtgcgtt ctcctcgaac gccgcacgca
     1081 gctgacgttc cgcgcgattg cgcgcagtga cgtccttcga agcgacgagc agcgcgacga
     1141 cggcgcccgt ggaatcgcga tcgccggttg ccgagacttc gacctcgagc acgtcgcccg
     1201 acttcgccac gtacgtgcgc ggctcgtcct cgagctcgcc cgtcgcgatg acgtcttcga
     1261 ggcgtccacc cgcaagcctc gcctgcagct ccttgcccat gaactcggtg atgacgcggc
     1321 ccaacacttc ctcgcggcga tagccgaggc tctcgagcca tctgtcgctg acgttcgtga
     1381 tgcggccgtg cttgtcgacc gtgtgcagca tggccggcgt cgcgcgatag agctcgcggt
     1441 agtgctgctc gaggcgcgcc tgctggccga cctccgtgta caccgcgatc gtgcgcagat
     1501 agtcgcccgc gtcgctgcgc tcgacgatcg cattcgcgag gcaatcgacg cgctcgttgt
     1561 tgcgcgtcac gagatcgatc ggcacgccgg cgagcttgcc cgtgcgccgc agcaccggca
     1621 gatactcttc gacgatccgc ttcgctccct cggggctcgc gaggtcttgc ggccgcatgt
     1681 tcgtgatctc ctcgcgcgcg taaccgaagc ggcgcagcca cgcgtcgctg gcatccacga
     1741 agaagccgtc ctcattcaat gagacgagca gggccgggct gcggcggaac agccattggt
     1801 attcgtcttg ttgcgacacg accgcacctt acggttttcc gtggctcatt gcaacggaaa
     1861 tccgtaattc acggaatatc gtgagtcgcg ggttactcta agtgactgaa gataggggtt
     1921 ttttcagttg gcacgcagca tgcaataacc ggctcgtgat ctcagacgag atcgtaggac
     1981 tgaagggatc ggtgaaggat tcagtatggt tgcacgcaag ctactgacgg cgactgccgc
     2041 tgggttggcc gcgagcctct tcgcacagca tgcgacgggc gccga
//
LOCUS       DASSNN010000427         3716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1459633, whole genome shotgun sequence.
ACCESSION   DASSNN010000427 DASSNN010000000
VERSION     DASSNN010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459633"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFL84_07985"
     CDS             <1..535
                     /locus_tag="VFL84_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608598.1"
                     /translation="SRERGAPVEWFFTPEGHAAHPAVVKRTAVERGGVGMIDLAALCH
                     AEQAACDRLLDDFRQQHELEQAAARTERVTLDVTIALDDHERLRVERLLAEEGMAAEI
                     RFTDVLKIVIVPTLGDDNRVLLWTAVYEFDGSDYVPLGEPQLASPGSGTVKLEMASTS
                     GSRFGLSLASLVAHAAE"
     gene            612..1232
                     /locus_tag="VFL84_07990"
     CDS             612..1232
                     /locus_tag="VFL84_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET7608599.1"
                     /translation="MISARKALEQLRAGNRRFVAERQRDGRTGATRREEVTERQEPFA
                     IVLGCSDSRVPAEIVFDQGLGDLFVIRVAGNIVAPSQIGSVEFAAEQFKTRLVVVLGH
                     TGCGAIDATIEQLTRPVEKQSRNLRAIVDRVRPSVEPLLATPLRDDPSALVRNAVRAN
                     VRASASHLRHGSEILEQLIERDGLLVVGAEYSLETGVVEFFEGLPE"
     gene            1323..2297
                     /locus_tag="VFL84_07995"
     CDS             1323..2297
                     /locus_tag="VFL84_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608600.1"
                     /translation="MQTRKRARLVSIAAGSLALAAFAVAAGAQDEPQAPQPQPQQQEQ
                     GPQRLTIRGTSVTLTAPPGFTASRTERGIENAATGTKITISEAAAEAYPGLADRFKSA
                     KSLTDGYADQNVTIRSVRTVDGKIPFAIGRQTKDGKDLTRYLALLKGDKTVLVSFTVP
                     DRTFTEADAEALLRSIELTPEPTLEERLAQLPFVLTAAPPYTVRDVINRQAVNLEVKG
                     DRAQPAITIGYGRSQALMGEEARVAVELIKSTGGYREAQITSQEPVTFAGGKGYRVTA
                     VIEDRTAVQYLRIVSGGGYIRLFARGTTSAMQNAEQVIAEIAGSVEPR"
     gene            2412..2960
                     /locus_tag="VFL84_08000"
     CDS             2412..2960
                     /locus_tag="VFL84_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7608601.1"
                     /translation="MARPARFRSWLGAAALVAVIGVAVYYGVTVNPSLLPVSPQSFAQ
                     PPYRSDVNARTALAEGRARAAASGKMLMVTFGANWCPDCLTLHRNLNAPETRAYVDEH
                     FEVVEIDVGSTKKNAAVQRDLGMSVNVIPLAVFYSSTGELVGDTYGGELRPSRHFSSR
                     EIRDFLREVVDYHRIVSPDQRQ"
     gene            2994..3560
                     /locus_tag="VFL84_08005"
     CDS             2994..3560
                     /locus_tag="VFL84_08005"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET7608602.1"
                     /translation="MFVIDSTVALRVLREDDAAELFALTDVNRAYLRRWLPWVDLVTS
                     EEDSRSFLTTVTAQREEGRGPTFAVLCDGSLAGVVGFLPVDRVNRIGEIGYWLAPPRQ
                     GRGVMTQCCRFVVRYGFLTLDLNRIQIAAGTENLESRAIPERLGFKFEGILRARENLY
                     GTFIDHAMYALLRSEFDEAEEKLALAGQ"
BASE COUNT          591 a         1267 c         1272 g          586 t
ORIGIN      
        1 gagccgcgag cgcggcgcgc ccgtcgagtg gttcttcacc ccggaaggcc acgccgcgca
       61 tccggccgtc gtcaagcgca cggccgtcga gcgcggcggc gtcggcatga tcgatctcgc
      121 ggcgctctgt cacgccgagc aggcggcgtg cgatcggttg ctcgacgact tccgccagca
      181 gcacgagctc gagcaagcgg cggcgcgtac cgagcgcgtc acgctcgacg tcacgatcgc
      241 gctcgacgac cacgagcgct tgcgcgtcga gcggctgctc gccgaggaag gcatggccgc
      301 ggagatccgc ttcaccgacg tcttgaagat cgtcatcgtg ccgacgctcg gcgacgataa
      361 ccgagtgctg ctctggacgg ccgtctacga gttcgacggc agcgactacg tgccgctcgg
      421 cgagccgcag ctcgcgagcc ccggcagcgg cacggtcaag ctcgagatgg cgtcaacctc
      481 aggtagtcgc ttcggcttgt cgctggcgtc gctcgtcgcg cacgcggccg agtagcccgg
      541 cgctccaacg gcggtctccc tcaatgctaa agttcgcggc cagcgcacga cgcgcgtaag
      601 gggagttgcg gatgatttca gcgcgaaagg cgctcgagca gttgcgtgcc ggcaatcggc
      661 gcttcgtggc agagcgccag cgcgacggcc gcaccggcgc gacgcgccgc gaggaggtca
      721 cggagcggca agagccgttc gcgatcgtgc tcggctgctc cgattcgcgc gtgccggccg
      781 agatcgtgtt cgatcagggt ctcggcgatc tgttcgtgat ccgcgtcgcc ggcaacatcg
      841 tggcgccgtc gcagatcggc agcgtcgagt tcgcggccga gcagttcaag acgcggctcg
      901 tcgtcgtgct cggccacacg ggctgcggcg cgatcgacgc gacgatcgag cagctgacgc
      961 gccccgtcga gaagcaatcc cgaaatctgc gcgcgatcgt cgatcgcgtg cgcccgtccg
     1021 tcgagccgct gctcgcgact ccgctgcgcg acgatccgag cgctctcgtc cgcaacgccg
     1081 tccgcgccaa cgtgcgcgcg tcggcgtcgc acttgcggca cggctccgag atcctcgagc
     1141 agctgatcga gcgcgacggg ttgctcgtcg tcggcgccga atactcactc gagaccggcg
     1201 tcgtcgagtt cttcgagggc ctgcccgagt aacgaccgaa ccgggggccg atttggcgtg
     1261 gtgctcgccg ccgactgaca atgctagagt gcctgcatcg cgcaaataga gcggggatct
     1321 ccgtgcagac acgcaaacgc gctcgcctcg tctcgatcgc agcaggctcg ctggcgcttg
     1381 ccgcctttgc ggttgcggcc ggcgcgcaag acgagccgca agcaccgcag ccgcagccgc
     1441 agcagcagga acaaggtccg cagcgcctaa cgatccgcgg cacgtcggtc acgttgacgg
     1501 cgccgccggg gttcacggcg tcgcgcaccg agcgcggcat cgagaacgcg gcaacgggca
     1561 cgaaaatcac gatctcggag gccgctgcgg aggcgtatcc ggggctcgcc gaccgcttca
     1621 agtccgcgaa gagcctgacg gacggctacg cggatcagaa cgtcacgatc cgctccgtgc
     1681 gcaccgtcga cggcaagatt ccgttcgcga tcggcaggca gacgaaggac ggcaaagacc
     1741 tcacgcggta cttggcgctg ctcaagggcg acaagaccgt gctcgtctcg ttcaccgttc
     1801 ccgatcgcac gttcacggaa gccgacgccg aggccctgct gcgctcgatc gagctgactc
     1861 ccgagccgac gctcgaggag cggctcgcgc agctgccgtt cgtgctgacc gccgccccgc
     1921 cctacaccgt cagggacgtc atcaaccgcc aggccgtcaa cctcgaggtc aagggcgatc
     1981 gagcgcaacc cgctatcacg atcgggtacg gccggtcgca agccttgatg ggcgaggaag
     2041 cgcgcgtcgc cgtcgagctg atcaagagca cgggcgggta tcgcgaggcg cagattacgt
     2101 cgcaggagcc ggtcacgttc gccggcggca aggggtaccg tgtcacggcc gtcatcgagg
     2161 accgcacggc cgtgcaatac ctgcgcatcg tctccggcgg cggctatatc cggctgttcg
     2221 cccgcggcac cacgagcgcc atgcagaacg ccgagcaggt catcgccgag atcgcagggt
     2281 cggtcgagcc ccgctagctt ccaacggttg cgaaggcctt gaaggttctt cggacacccg
     2341 gcgtgtcgcc ggcggccaac gcgtgtatcg ttcttttcgt gagcatccct cgcctcaacg
     2401 agagggcccg agtggcccga ccggcgcgat ttcggtcgtg gctcggcgcc gcggcgctcg
     2461 tcgcggtcat cggtgtggcc gtctactacg gggtcaccgt gaacccgtcg ctgctgccgg
     2521 tgagcccgca gtcgttcgcg caacccccgt accgctccga cgtcaacgcc cgaacggcgc
     2581 tcgccgaggg cagagcgcga gcggccgcga gcggcaagat gctgatggtc acgttcggcg
     2641 cgaattggtg cccggactgc ctcacgctgc atcggaattt gaacgcgccc gagacgcggg
     2701 cgtacgtcga cgagcacttc gaggtcgtcg agatcgacgt cggctccacg aagaagaacg
     2761 ccgccgtgca gcgcgatctc ggcatgtcgg tcaacgtcat tccgctcgcg gtgttctact
     2821 cgtcgacggg cgagctggtc ggcgacacat acgggggcga gctccggccc tctcgccatt
     2881 tctcctcgcg cgagatccgt gacttcctgc gcgaagtcgt ggactatcac cgcatcgtca
     2941 gccccgacca gcggcagtga gaatcgcgcg gcggcacgcc gcgctcgaag cgaatgttcg
     3001 tcatcgattc aacggtggcc ttgcgcgtgc tgcgcgagga cgatgccgcg gagctgttcg
     3061 cgctgacgga cgtcaatcgc gcgtatctgc gccgctggtt gccgtgggtc gatctcgtca
     3121 cgtccgagga ggactcgcgg tcgttcctga cgacggtgac tgcgcagcgt gaagagggcc
     3181 gcggcccgac gttcgcggtg ctctgcgacg gctcgctcgc gggcgtcgtg ggcttcctac
     3241 cggtcgaccg cgtcaatcgc atcggcgaaa tcggctactg gctcgcgccg ccacgccaag
     3301 gccgcggcgt catgacgcag tgctgccggt tcgtcgtccg ctacggcttc ctgacgctcg
     3361 acttgaatcg catccagatt gcggccggca cggagaatct cgagagccgc gcgattccgg
     3421 agcggctcgg cttcaagttc gaaggcatcc tgcgcgcccg cgagaacctc tacggcacgt
     3481 tcatcgacca cgcgatgtac gcgcttctgc gaagcgagtt cgacgaggcc gaggagaagc
     3541 tcgcgcttgc cggccagtag gcggtttgcc aaagtagccc gctatgtcgg caaacgcggc
     3601 cccgagacgg aagctctact ggctattggc cgtcgtggcc gcggccgtcg cgatcggagt
     3661 cgtggcttgg gcgcagctcg gcgcgcgcca tgcgccgacg gccgcggggt tcaagg
//
LOCUS       DASSNN010000428         4394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1306905, whole genome shotgun sequence.
ACCESSION   DASSNN010000428 DASSNN010000000
VERSION     DASSNN010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306905"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFL84_08010"
     CDS             complement(<1..268)
                     /locus_tag="VFL84_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET7608603.1"
                     /translation="MSETDEHAASNLQRSRRVVIKTMSAAGAAVLAPNWLSAQTAPPS
                     TVSVPPREFGPDAPPVTYPDPDIVTVDPAFNALRVGNTPIQRLWT"
     gene            338..2329
                     /locus_tag="VFL84_08015"
     CDS             338..2329
                     /locus_tag="VFL84_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HET7608604.1"
                     /translation="MQISPRVLLPLLIASLLSACSPPSGGESQTQAAAPAPAGGAASA
                     GTAAAAPGPMVEWRFYGGNLASQRYSPLDQIDASNAGSLKVAWRFNTGNFGPRPEARN
                     ETTPLMIDGVLYTQAGITRNVVAINPKSGELLWVWRPDDGEQRFARAPRKTSGRGPAY
                     WSDGAGNERLLTVTPGFNLVALDPDTGRPVPGFGESGVVDLMIGTRGGVDDKSSIGNS
                     SPALVIGDVVVVGPAHEVGMRPPSQRNFKGDVRGFDVHTGKLLWTFHTIPEAGEPGYE
                     TWLDGSAERTGNAGVWAAMTADAELGYVYLPTEAPLADIWGGERHGNNLYSSSLVCLD
                     AKTGERVWHYQLIHHDIWDWDNPTAPILMDLVVDGRPIKAVAQLTKQAFVYTFDRVTG
                     EPVWPIEERPVPQTDVPGEWTSPTQPFPTKPPPYDRQGVTIDDLIDFTPELRAEAVEG
                     IKKFRIGPIFTPPSLANASDGTSGTLTLPNATGGANWEGGAFDPETNVLYVGSVTNPS
                     IFALQPPPEPTDITYIAGGGAQLPWLQGLPLIKPPYGRITAYDMNKGEILWQRASGPT
                     PKAIAENPALKGLDIPPTGTAARAQTLVTKTLLFTADGWGGTPFLRAHDKATGAVLAE
                     VALPGAVGGRPMTYMIDGKQYVVVSVAGDNGAELVALTL"
     gene            2489..3826
                     /locus_tag="VFL84_08020"
     CDS             2489..3826
                     /locus_tag="VFL84_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HET7608605.1"
                     /translation="MSAVANAVPSASRTDPDRSIDELDAAICRLAGRINAVTYQLLVL
                     VREFDDRMGWAKWGHRSCAEWLSWRCQISLSAAREKVRTAQALRDLPAISLAFREGRL
                     SYTKARALTRVALEHGEEALLRYALDASAPAVEERCRQIRNVHPESVHDARRAWEARA
                     LSAWNNAARGTLCLRAELPLELGELVMKAIERALEREEIPDGVADRAPSSFPSQQADA
                     LVAIMTTYLEGGAVEPGSSSTADRYQVVLHVDEAALHGGAGRADAPLETVKRLACDAS
                     LVVVTEDERGTPLGVSPKQRVVSTPLRRALVARDRRCAFPGCPCTKFLQAHHVEHWVE
                     GGETTLRNLLLLCPYHHRLLHEGGYRIGRDYQGELYFMRVDGRAIPRCGYRVDDYTDD
                     YPAGNPSNAESDYESPSMEDSPHVDADHATPSMEVREPRGVYRLKAQRSALLV"
     gene            complement(3827..4228)
                     /locus_tag="VFL84_08025"
     CDS             complement(3827..4228)
                     /locus_tag="VFL84_08025"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7608606.1"
                     /translation="MTVKAAALAVALAALVAGTAAGHHSFSAEFDPDRPVKFTGKVTE
                     MKWSNPHAWIYLDVTGDDGKVVNWALEMTGANGLFRRGWRKEDLPAGTVLQVEGWQAR
                     NATPTANIVSVTFEDGRTLFAGSSNPAARQQ"
BASE COUNT          701 a         1478 c         1527 g          688 t
ORIGIN      
        1 cggtccacag gcgctggatc ggcgtattgc cgacgcgcag cgcgttgaac gcgggatcga
       61 cggtcacgat gtccgggtcg ggatacgtga cgggcggcgc gtcgggaccg aactcgcgcg
      121 gcggcacgga caccgtcgaa ggcggcgccg tttgcgcaga gagccaattc ggcgcgagga
      181 cggccgcgcc ggcggcgctc atcgtcttga tcacgacgcg cctcgatcgt tgtaggttgc
      241 tagccgcgtg ctcatcggtt tcgctcacgt gccgactccc tgcgcttccc ggcctcagtg
      301 tagagtagcc gtgcccggaa aagtcagggg ggaaaccgtg cagatctcgc cgcgcgtcct
      361 attgccgttg ctcatcgcct ctctattgag cgcgtgctcg ccgccgtccg gcggagaatc
      421 gcagacgcag gccgcggcgc cggctcccgc cggcggcgct gcgtcggccg ggaccgcggc
      481 cgccgcgccg ggcccgatgg tcgagtggcg gttctacggg ggtaacttgg cgtcgcagcg
      541 ttattcgccg ctcgatcaga tcgacgcgag caacgcgggc agcctcaagg tcgcatggcg
      601 gttcaacacc gggaatttcg gcccgcgccc cgaagcgcgc aacgagacga cgccgctgat
      661 gatcgacggc gtgctctata cgcaggccgg gatcacgcgc aacgtcgtcg cgatcaaccc
      721 caagagcggc gagctgctgt gggtctggcg ccccgacgac ggcgagcagc gcttcgcgcg
      781 cgcgccgcgc aagacgtcgg gccgcggccc cgcgtattgg agcgacggcg ccggcaacga
      841 gcggctgctc acggtcacgc cgggcttcaa cttagtcgcg ctcgatcccg acacgggccg
      901 gcccgtgccc ggcttcggcg agagcggcgt cgtcgacctc atgatcggca cgcgcggcgg
      961 ggtcgacgac aagtcgagca tcggcaacag ctcgcccgcg ctcgtcatcg gcgacgtcgt
     1021 cgtcgtgggc cccgcgcacg aggtcggcat gcggccgccg tcgcagcgca acttcaaagg
     1081 cgacgtgcgc ggattcgacg tgcacaccgg caagctgctc tggacgttcc acacgattcc
     1141 ggaagccggc gagcccggct acgagacctg gctcgacggc tcggccgagc gcacgggcaa
     1201 cgccggcgtc tgggccgcca tgacggccga cgcggagctc ggctacgtct atctgccgac
     1261 cgaagcgccg ctcgccgaca tctggggcgg cgagcgccac ggcaacaatc tctacagctc
     1321 gagcctcgtg tgcctcgacg cgaagaccgg cgagcgcgtc tggcactacc agctcattca
     1381 ccacgacatt tgggactggg acaacccgac cgcgccgatc ctgatggacc tcgtcgtcga
     1441 cggccggccc atcaaagccg tcgcgcagct cacgaagcag gcgttcgtct acacgttcga
     1501 tcgcgtgacg ggcgagcccg tctggccgat cgaggaacgg cccgtgccgc agaccgacgt
     1561 gcccggcgaa tggacctcgc cgacgcagcc gttcccgacg aagccgccgc cgtacgatcg
     1621 acagggcgtg accatcgacg atctgatcga cttcacgccg gagcttcgcg ccgaggccgt
     1681 cgagggcatc aagaagttcc ggatcgggcc gatcttcacg ccgccttcac tcgcaaatgc
     1741 ctcggacggc acgagcggca cgctgacgct gccgaacgcg acgggcggcg cgaactggga
     1801 aggcggcgcc ttcgatcccg agacaaacgt gctctacgtc ggctcggtca cgaatccttc
     1861 catcttcgcg ctccagccgc cgccggagcc gacggacatc acgtacatcg cgggcggcgg
     1921 cgcgcagctg ccgtggctgc aaggattgcc gctgatcaaa ccgccgtacg gtcgaatcac
     1981 ggcgtacgac atgaacaaag gcgagatcct ctggcagcgc gcgagcggcc cgacgccgaa
     2041 ggcgatcgcc gagaacccgg cgctgaaagg gctcgacatc ccgccgaccg gaacggcggc
     2101 gcgcgcgcaa acgctcgtga cgaagacgct gctgtttacg gcggacggct ggggcggcac
     2161 gccgttcttg cgcgcgcacg acaaggcgac gggcgcggtc ttggcagaag tcgcgcttcc
     2221 tggagcggtc ggcgggcggc cgatgacgta catgatcgac ggcaagcagt acgtcgtcgt
     2281 ttcggtggcc ggcgacaacg gcgcggagct cgtggcgctg acgttgtaga gcggcgaagc
     2341 ggcgaacaag agcgtcgtgc tcacgcgagc gcgcgtgtct atttcggcgc gcgaatctcg
     2401 gcgttcttat caaaaatcgt tattgggcgg tgggttagaa gctggataaa ataccagcac
     2461 atccaccgat atcccattgg agaccgccat gagcgccgtt gccaacgccg tgccgtccgc
     2521 gtctcgaacc gatcccgacc gctcgatcga cgagctcgac gcggcgatct gccgcctcgc
     2581 cggccgcatc aacgccgtga cctaccagtt gctcgtgctg gtgcgcgagt tcgacgaccg
     2641 catgggttgg gcgaagtggg gccaccggag ctgcgccgag tggttgtcgt ggcgctgcca
     2701 gatttcgctg tcggccgcgc gcgagaaggt gcgcacggcg caggcgctgc gcgatctgcc
     2761 ggcgatctcg ctcgcgtttc gcgagggccg gttgtcgtac acgaaggcgc gcgcgctgac
     2821 gcgtgtggca ctggagcacg gcgaggaggc gctcctgcgc tacgcgctcg atgcgtcggc
     2881 gccggccgtc gaggagcgct gccggcagat ccgcaacgtg catccggagt ccgtgcacga
     2941 cgcgcggcgc gcgtgggagg cccgcgcgtt atcggcgtgg aacaacgccg cgcgcggcac
     3001 gctgtgcttg cgggcggagc tgccgctcga gctgggcgag ctcgtcatga aggcgatcga
     3061 gcgggcgcta gagcgcgaag agattcccga tggcgtcgcc gaccgggcgc cgtcgagctt
     3121 tccgagccaa caggccgatg cgctggtcgc gatcatgacg acgtatctcg aaggcggcgc
     3181 cgtggagccg ggctcatcgt cgacggccga tcgctatcaa gtcgtcttgc acgtcgacga
     3241 agcggccctc catggagggg cggggcgggc cgacgcgccg ctcgagacgg tgaagcggct
     3301 cgcctgcgat gcgagcctcg tcgtcgtgac ggaggacgag cgtggcacgc cgctcggtgt
     3361 gagcccgaag cagcgggtcg tgtcgacgcc gctgcggcgt gcgctcgtgg cgcgcgaccg
     3421 ccgctgcgcg ttcccggggt gtccctgcac gaagttcctc caggcgcacc atgtggagca
     3481 ttgggtcgag ggcggggaga cgacgttacg gaaccttctg ctgctgtgcc cgtatcatca
     3541 tcggctgctg cacgaaggcg gctatcgcat cgggcgggac tatcaaggcg agctgtactt
     3601 catgcgcgtg gacggacgcg cgattccgcg ctgcgggtat cgcgtggacg actacaccga
     3661 cgattatccg gctggaaatc cctccaatgc cgaaagcgat tacgaaagcc cctccatgga
     3721 ggactcgcct catgtcgatg ccgaccacgc cactccctcc atggaggtgc gcgagccgcg
     3781 cggcgtttat cggctgaagg cgcagcgcag cgccttgctc gtctgactac tgctggcgag
     3841 cggccgggtt cgacgagccc gcgaacagcg tgcgaccgtc ctcgaacgtc acgctgacga
     3901 tgttcgccgt cggcgtcgca ttgcgcgcct gccagccctc gacttgcagc accgtgccgg
     3961 ccggcaaatc ttccttgcgc cagccgcgcc ggaacaggcc gttcgcgccg gtcatctcga
     4021 gcgcccagtt cacgactttg ccgtcgtcgc cggtgacgtc caagtagatc caagcatgcg
     4081 ggttggacca cttcatctcg gtgaccttgc cggtgaattt caccgggcgg tccggatcga
     4141 actcggccga gaacgagtgg tgcccggcgg ccgttccggc cacgagcgcg gcgagagcga
     4201 cggcgagcgc tgcggccttg actgtcatga cgctaattcc cctgctgctt ctgactcgcg
     4261 cggtactcgt cgcgcacgtg cctcaagtgc tcgagatcct cgttgtcctc gtggcacgcg
     4321 acctcgagca gctcgtcggc cttgcgatgc atttgcacct cgatcgtcca cgggcgagtg
     4381 tacgggatcg ggtc
//
LOCUS       DASSNN010000429         5785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_938808, whole genome shotgun sequence.
ACCESSION   DASSNN010000429 DASSNN010000000
VERSION     DASSNN010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938808"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2833
                     /gene="hrpA"
                     /locus_tag="VFL84_08030"
     CDS             <1..2833
                     /gene="hrpA"
                     /locus_tag="VFL84_08030"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HET7608607.1"
                     /translation="NIAETSLTVPRIRYVIDSGLARVSRYGTRQRVQSLGVEPIAQAN
                     AAQRAGRCGRLAPGICVRLYSEEDFAARPPFTEPEILRTGLAGVLLRLEALRLGPIDE
                     VPFIDAPPKKAVADAYQLLQLLGAIDDERRLTKDGELMARLPVDPRVARLLVVANKND
                     ALREGLVIAAALSVVDPREYGVDPDAARRKHEVFADARSEFTSYLNLWSAYRRERRGG
                     ERALRKWAKDHYLSVARLREWHDVHDQLHELVQALRWRTRQQAADYRAVHQAVLAAFI
                     DFIAEHDDGLVYRGMRESRAQLFAGTPLAKKRPRWVVAAERVATERQYLRTVAQVNPR
                     WALRVAPHLVRFEYDDPQWDAERGQVTAREVATLFGMRIASERRIDYGRVDAKEARRI
                     FIAEALAADEAGFEAGEPEFLAHNRRVRRAVLDAEARLRKRDLFVGEGGVAAFYEQRL
                     PAGVHDRASLKSVGRGHELFMTLRDVAARDPGDLAALGFPAELTLAGHRLPLTYVFEP
                     GTDADGITVTVPRSLLGAIAPEEIEWLIPAWLHDKVVAYLRALPKERRRGLVPLPDTA
                     REALAAMAATAGRQPVSIALAEALREVRRANVAPSAFDERALPPHFRMRVAVVDAEGR
                     MLAASRDVAALQRELGAPPGVPAVEAAPAHWQRAGLTRWDVGDLPQTVAVPQRPQPLQ
                     LYPALVDRDGRVDLTLQPPGPAAVALHRGGVRRLLLKALPQQAALIRDRILADRALVL
                     AYHGVADGAALVDDVLLAAAEQAFALDPPVRTELEFAALLKRGRAQLVPEADELRALL
                     DEILPLQRSLRRTLDAAAKKGAPTSTHSELAAQLAELVGPRMLTDTPREWRQHLPRYL
                     RAAEQRWEKRGQRNEPELAAAARTAAARLDHWRATRPPGTPWPEGVVEYRWLLEEFRV
                     SLFAQQLGTVRSVSAKRLEQAWRKATASA"
     gene            complement(2879..5134)
                     /locus_tag="VFL84_08035"
     CDS             complement(2879..5134)
                     /locus_tag="VFL84_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608608.1"
                     /translation="MTARPLLSLALLATAASLAACGGGGGGGGGGGNGGGGGGSGGGG
                     GQTGITVSPTTLSFTANHDDPNPPPAQTVTVTAHESGTTWLMFEGGSTAGIACNNGAS
                     CTVEVRPPNAPSSFGTPGTTTNGVTLLGCADQFCVSSGQRGRVTITYTYTVTPGFNIL
                     PETVPTVFGVEGVQTPPLQLTLTNPSGTVQPLYVLNQNLGNPVFATVTPPPGPVPSGT
                     ALEVLFANNLPVGSYTGTLEVQSGASTRFTRRTINYVVTSAYRVSALATFIVDGTTTM
                     SGLEQTVVVSSQAPGTSFNWTATEDSPWLELSRTSGDTSTNNDIELGLPIAALERIPT
                     GVHRATVTIGPPPATPPPAPTAYSFDVVLDMRLPLVDFAMPKAVVAGDTGEVYIKGAG
                     FPAPLSGVSFGGSPATAFTRESDARIRAAYPALPNASHDVTVVGLQSNALIDRQHAAV
                     FAAGSALPPAAELTSAGRKYTAIYDDERSTLYLANTDNAEIERYVFSGTWSAATSVPV
                     PALRDLMLAPDGRLLALTASALVTIDANTLATTSLTTPTFQGTLPNRTFASGIDGEIL
                     IARADDLATSSNIIRYVPSTGAFTDEFFCCLDRYIRASGDGTLLYATSNGGVQRYDVR
                     TRTYQQGASAPGKRSLSVTRDGAKFLLDATRLFSSTFELLGDLGVPDAMTGAVTGDGR
                     RAYVYSSASPLAAQLYAFDLTGPPPFAQLAQSPIALPDSVGNEPVMTTSVDGRVVFIA
                     GSGRVIIKSVP"
     gene            complement(5252..5653)
                     /locus_tag="VFL84_08040"
     CDS             complement(5252..5653)
                     /locus_tag="VFL84_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608609.1"
                     /translation="MPFAVALLCHPLGTFAALVATIAAGLFAAQSRRFLPAFTAASAA
                     SLTLLAFAQVPPNVSGLAWLFLGLVLLHAEFFWPTYGLAGLLGIGAAAWGSCLLLLPL
                     PPVPRGAAALLGAVMLLAAVARTARLRTLPR"
BASE COUNT          874 a         1948 c         2111 g          852 t
ORIGIN      
        1 gaacatcgcg gaaacatcgt tgacggttcc gcgcatccgc tacgtcatcg actccggctt
       61 ggcgcgcgtg agccgttatg ggacgcggca gcgcgtgcag agcttgggcg tggagccgat
      121 cgcgcaagcc aacgcggcgc agcgcgcggg gcgctgtggc cgtctggcgc ccggcatttg
      181 cgtgcgtttg tactcggagg aggacttcgc ggcgcggccg ccgttcacgg agcccgagat
      241 cttgcgcacg gggctcgccg gcgtcttgct gcggctcgag gcgctgcggc tcgggccgat
      301 cgacgaagtc ccgttcatcg acgcgccgcc gaagaaggcg gtcgccgacg cgtaccagct
      361 gctgcagttg ctcggcgcca tcgacgacga acggcggctc acgaaggacg gcgagctcat
      421 ggcgcggctg cccgtcgatc cgcgcgtcgc gcgcttgctc gtcgtcgcga acaagaacga
      481 cgcgctgcgc gaaggtctcg tgatcgcggc cgcgctcagt gtcgtcgatc cgcgcgagta
      541 tggcgtcgac ccggatgcgg cgcggcgcaa acacgaagtt tttgcggacg cgcgctccga
      601 gttcacgagc tatttgaatc tttggagcgc gtaccgccgc gagcgccgcg gcggcgagcg
      661 cgcgttgcgc aaatgggcga aggatcacta cctgtccgtc gcgcgcttgc gcgagtggca
      721 cgacgtgcac gaccagctgc acgagctcgt gcaggcgctg cgctggcgca cgcggcagca
      781 agccgccgat taccgcgcgg tccatcaggc cgtgctcgcg gcgttcatcg acttcatcgc
      841 cgagcacgac gacggtctcg tctatcgcgg catgcgcgag tcgcgcgcgc agctctttgc
      901 cggcacgccg ctcgcgaaga agaggccgcg ctgggtcgtc gccgccgagc gcgtcgcaac
      961 ggagcgccaa tatctgcgga cggtcgcgca agtgaatccg cgctgggcgc tgcgagtcgc
     1021 gccgcatctc gtgcggttcg agtacgacga cccgcagtgg gacgcggagc gcggtcaggt
     1081 gacggcgcgc gaggtcgcga cgctgttcgg catgcgcatc gcgagcgagc ggcgcatcga
     1141 ttacggccgc gtcgatgcga aggaagcccg gcggatcttc atcgcggagg cgctcgcggc
     1201 cgacgaggcc gggttcgagg ccggagagcc cgagtttctc gcgcacaacc gccgtgtccg
     1261 ccgcgcggtg ctcgatgccg aggcgcgact gcgcaagcgc gacctgttcg tgggcgaagg
     1321 cggcgtcgcg gcattctacg agcagcggct gccggccggc gtgcacgacc gcgcgagcct
     1381 aaagtccgtc ggccgcgggc acgagctctt catgacgctg cgcgatgtcg ctgcgcgcga
     1441 ccccggcgat ctcgcggcgc ttgggtttcc ggccgagctg acgctcgccg gccataggct
     1501 gccgctgacc tacgtcttcg agcccggcac cgatgccgac gggatcacgg tcacggtgcc
     1561 gcgctcgctg ctcggcgcga tcgccccgga agaaatcgag tggctgattc cggcgtggct
     1621 gcacgacaaa gtcgtcgcgt accttcgcgc gctgccgaaa gagcggcggc gcgggctcgt
     1681 gccgctcccc gacacggcgc gcgaggcgct cgcggcgatg gccgcgacgg cgggccgcca
     1741 gcctgtgtcg atcgcgctcg cggaggcgct gcgcgaggtg cggcgggcga acgtagcgcc
     1801 gagcgcgttc gacgagcgcg cgctgccccc acatttccgc atgcgcgtcg ccgtcgtcga
     1861 cgccgagggc cgcatgctcg cggcgagccg ggacgtcgcg gcgctgcagc gcgagctcgg
     1921 cgcgccgccc ggggttcccg ccgtcgaagc ggcgccggcg cactggcaac gcgcgggact
     1981 cacgcgctgg gacgtcggcg atcttccgca aacggtcgcc gtcccgcagc ggccgcaacc
     2041 gttgcagctg tatccggcgc tcgtcgaccg cgacgggcgc gtggacctta cgctgcagcc
     2101 gcccgggccg gcggcagtcg cgctgcaccg cggcggcgtt cgccggctgc tgctcaaagc
     2161 gctgccgcag caggcggcgc tgattcgcga ccgcatcctc gcggaccgcg cgctcgtgct
     2221 cgcgtatcac ggcgtcgccg acggcgcggc gctcgtcgac gacgtgctgc tcgcggccgc
     2281 cgagcaagcg ttcgcgctcg atccgcccgt gcgcacggaa ttggagttcg cggcgctgct
     2341 gaagcgcggc cgagcgcagc tcgttcccga agcggacgag ctgcgcgcgc tgctcgacga
     2401 gatcctgccg ctgcagcgct cgttgcggcg taccctcgat gcggccgcga agaagggcgc
     2461 gcccacgagc acgcactccg agctcgccgc gcagctcgcc gagctcgtcg gcccgcgcat
     2521 gctgacggac actccgcgcg agtggcggca acacttgccg cgctacttgc gcgccgcgga
     2581 gcagcgctgg gaaaagcgcg ggcagcgcaa cgagcccgag ctcgccgccg cggcgcgaac
     2641 ggccgcggcg cggctcgatc actggcgcgc tacgcgcccg cccggcacgc cgtggcccga
     2701 gggcgtcgtc gagtaccgct ggctgctcga ggaatttcgc gtgtcgctgt tcgcccagca
     2761 gctcggaacg gtgcgctccg tatcggcgaa gaggctcgag caggcgtggc gcaaggcgac
     2821 cgcaagcgcg tgagcgccgt cgccgttggc cttgcccacc gagcgcgtga gtgcgctctt
     2881 acggcaccga cttgatgatg actctgccgc tgccggcgat gaagacgacg cggccgtcga
     2941 cgctcgtcgt catgacgggc tcgttgccga cgctgtccgg caatgcgatc ggggactgcg
     3001 cgagctgcgc gaacgggggc gggccggtca agtcgaacgc gtacagctgc gccgcgagcg
     3061 gcgatgcgct cgagtagacg tacgcgcggc ggccatcgcc cgtaacggcg cccgtcatcg
     3121 cgtccggcac accgaggtcg ccgagcagct cgaacgtgga gctgaagagt cgcgtcgcgt
     3181 cgagcaggaa cttcgcgccg tcgcgcgtca ccgagaggct gcgcttcccc ggcgcggacg
     3241 cgccttgctg gtaggtccgc gtccgaacat cgtagcgctg cacgcctccg ttgctcgtcg
     3301 cgtagagcag cgtgccgtcg ccgctcgcgc ggatgtagcg gtcgaggcag cagaagaatt
     3361 cgtccgtgaa cgcgccggtg gacggtacgt agcggatgat gttcgaggag gtggcgagat
     3421 catcggcccg cgctatcaaa atctcgccgt cgatgccgct cgcaaaggtg cgattcggca
     3481 gagtaccctg aaacgtcggc gtcgtgagag aggtcgtcgc caaagtgttt gcgtcgattg
     3541 tcacgagagc gctcgcggtc aatgcaagaa gccgcccgtc cggcgccagc atgaggtcgc
     3601 gcaaggccgg tactggtacc gacgtcgcgg cgctccacgt gccgctgaaa acataacgct
     3661 cgatctcggc gttgtccgtg ttggccaggt agagcgtgct gcgctcgtcg tcgtagattg
     3721 ccgtgtattt gcggccagcc gacgtcagct ccgcggcggg agggagggcc gagcccgcgg
     3781 cgaagaccgc tgcatgctgg cgatcgatca aagcgttcga ctgcaatccg acgaccgtga
     3841 cgtcgtgact ggcgttcggt agcgccggat acgcggcgcg gatccgtgcg tcgctctcgc
     3901 gggtgaatgc cgtcgccggc gagcccccga acgagacgcc gctgagcggc gcgggaaaac
     3961 cggccccctt gatgtagacc tctcccgtgt ctccggcgac gacggccttc ggcatcgcga
     4021 agtcgacgag cggcagacgc atatcgagca ctacatcgaa cgagtaggcc gtgggagccg
     4081 gaggcggcgt cgcgggtggc ggtccgatcg tgacggttgc gcggtgaacg cccgtcggaa
     4141 tgcgctccag cgccgcgatt ggcaagccga gctcgatgtc gttgttggtc gacgtgtcgc
     4201 cgctcgtccg cgatagctcg agccatggcg agtcttcggt cgccgtccag ttgaagctcg
     4261 tgcccggcgc ctgcgagctg acgacgaccg tttgctcgag gccggacatg gtggtcgtgc
     4321 cgtcgacgat gaacgtcgca agcgccgata cgcgatacgc cgaagtgacg acgtagttga
     4381 tcgtgcggcg ggtgaagcgc gtgctcgcgc cagactgaac ctcgagcgta ccggtgtagc
     4441 tgccgacggg caggttattc gcgaacagta cctcgagcgc cgtgccgctc ggcaccggtc
     4501 ccggtggagg cgtcaccgtc gcgaacaccg ggttgccgag gttctggttc agcacgtata
     4561 gcggctgcac ggtgccgctc ggattcgtca acgtgagctg cagcggcggc gtctgcacgc
     4621 cctcgacgcc gaagaccgtc ggcaccgttt cgggcaggat gttgaagccg ggcgtgaccg
     4681 tatacgtgta cgtgatcgtc acgcggcctc gttgaccgct cgaaacgcag aactggtcgg
     4741 cgcagccgag cagcgtcacg ccgttcgtcg tcgtgccggg agtcccgaag ctcgacggcg
     4801 cgttcggcgg tcggacttcc accgtgcagc tcgcgccgtt gttgcaggcg atgccggccg
     4861 tcgagccgcc ttcgaacatc aaccaggtcg ttcccgactc gtgcgccgtg accgtgacgg
     4921 tctgtgccgg cggcggattc ggatcgtcgt gattggccgt gaaagacagc gtcgtcggcg
     4981 acaccgtgat gccggtttgg ccgccgccgc cgccacttcc gccgccgccg ccgccgttac
     5041 cgccaccgcc accgcccccg ccgccgcctc cgcacgcggc gagtgaagca gccgtcgcga
     5101 gcagcgcgag cgagaggagc ggtcgagccg tcatcgaaag gcaagggaag ggcgcgcggg
     5161 caattcgaag ctcgttcatc ggttgttatg gttgcttatg gaggagaacg gcggcgccga
     5221 gaatagcacg gcgctcgaac gctgccgctg gctaccgcgg caatgtgcgg agccgcgccg
     5281 tgcgcgcgac agcggcaagc aacataacgg cgccgagcag cgcggcggcg ccgcgaggaa
     5341 ccggcggcag cgggagcagc aacaagcagg atccccatgc cgcggcgccg atgccgagca
     5401 aaccggcgag gccgtacgtc ggccagaaaa attctgcgtg cagcagcacg agcccgagga
     5461 acagccacgc gagcccactg acattcggcg gcacctgcgc gaacgcgagc aacgtcagcg
     5521 aagcggcaga cgcagccgtg aacgccggca ggaaccggcg cgactgcgcg gcgaatagcc
     5581 ccgcggcgat cgtcgcgacg agcgcggcga acgtaccgag cggatggcag agcaaggcga
     5641 cggcgaacgg catcggggct cctcgatggg acggtaggct tgcccagttg cggcggcgaa
     5701 gcgccgtttc cgggcggtgc gaggagtttc tttggccgcc gcgcggacgg gcggtatagt
     5761 gcccgcgtgc cgcgttgcgc aggca
//
LOCUS       DASSNN010000430         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1307096, whole genome shotgun sequence.
ACCESSION   DASSNN010000430 DASSNN010000000
VERSION     DASSNN010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307096"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="VFL84_08045"
     CDS             <1..157
                     /locus_tag="VFL84_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HET7608610.1"
                     /translation="AAAIRARADALRANADLVQLQAVEKWDGKLPTTMVPSSALPFIN
                     VQPTARN"
     gene            254..1192
                     /locus_tag="VFL84_08050"
     CDS             254..1192
                     /locus_tag="VFL84_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7608611.1"
                     /translation="MLTRREYLALTARAGAALALPQALLAQAGEVLTRPIPKSGERLP
                     VVGLGSSATFSQIAGAADIEAIKAVFSAMLEHGGTVFDTAPGYGASEEAAAKAVNELG
                     VRDRVFWATKLNVAGRGAGADPARARAQVENSFTRLGRDKIDLIQVHNVASMDIQFPI
                     LEEYKEAGRVRYVGTTSTFKPQYEALYQLMREKPFDFIGIDYAVDNVDAEERIFPLAL
                     DRGIGVLVYLPFGRDRLWDRVEGHAVPEWAAEFGATTWAQFFLKFAASHPAVTAVTPA
                     TSKPHHMVDNMTAARGRLPDQAERKRMVDFIAALPS"
     gene            complement(1170..2105)
                     /locus_tag="VFL84_08055"
     CDS             complement(1170..2105)
                     /locus_tag="VFL84_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7608612.1"
                     /translation="MRGSPISERSPPIAGALFGALAALIWAGFPAITKLSMASNALDA
                     WDITALRFGVGGLLLLPLFTRRRLGSLSPLTALFLACGAGAPYVLLTAGGLAFAPGGH
                     MGVMTPSCMLLCSTLGSRLILKEPLTGGRIAGALAIAAGLVALGWDGLQNHGELTWLG
                     DAMFVLGGLLWASYTIGLRAWRVEPVHATAIVGVVSMVLYLPGYAWLGGADLAAAPWR
                     EIVIQAVFQGVLSAVVALLLYTRAVAILGAARGAVFAALVPAFSLLLAVPMLHETPSE
                     LQLVGVVFVTAGMVFALGLHDPKKWRALPSSAAPR"
     gene            complement(2086..>2615)
                     /locus_tag="VFL84_08060"
     CDS             complement(2086..>2615)
                     /locus_tag="VFL84_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET7608613.1"
                     /translation="KARRVVTVHRTTPEGLVETPNPAEITAPVYGDGGLHSTAADYAK
                     FIQLFLNDGRAPNGKRLLSEATVALMGENHIGAIFVEQQPAALPALTEPFPLGAGRDK
                     FGLGFQITGPHSDKLSRSPGSMSWAGIFNTEFWIDPARGIGGVLLMQYLPFYDGDAIA
                     TLQGFERRVYQGLAD"
BASE COUNT          461 a          883 c          901 g          370 t
ORIGIN      
        1 ggccgcggcg attcgcgcgc gcgccgacgc gctgcgcgcg aacgccgatc tcgtgcagct
       61 gcaagccgtc gagaagtggg acggcaagtt gccgacgacg atggtgccga gctcggcgct
      121 gccgttcatc aacgtgcagc cgacggcgcg caactagcgg ccgctaaact ggagattcca
      181 taggcgcagg tccgcgcgcg cggccccgat gctatttttc ggtcttccgc cgagaaagta
      241 gagggagcac gcgatgttga cgcgacggga gtatctggct ctgacggctc gagcgggcgc
      301 cgcactggcg ctgccgcaag cgctgctcgc gcaggcgggc gaggtgctga cgcggccgat
      361 cccgaagagc ggcgagcggt tacccgtcgt cggcttgggc agctccgcga cgttcagcca
      421 gattgcaggg gccgccgaca tcgaagcgat caaagccgtg ttcagcgcga tgctcgagca
      481 cggcggaacc gtcttcgaca cggcgcccgg gtatggcgcg tcggaagagg ccgcggcgaa
      541 ggccgtcaac gagctcggcg tgcgcgaccg cgtgttctgg gcgacgaagc tcaacgtcgc
      601 cgggcgcggc gccggcgctg atcccgcgcg tgcgcgcgcg caagtcgaga attcgtttac
      661 gcgtctcggc cgcgacaaga tcgatttgat ccaagtgcac aacgtcgctt cgatggacat
      721 tcaattcccg atcctcgagg aatacaagga agcgggccgc gtgcgctatg tcggcacgac
      781 gtcgacgttc aagccgcaat acgaagcgct gtaccagctc atgcgcgaga agccgttcga
      841 tttcatcggc atcgactacg ccgtcgacaa cgtcgacgcc gaggagcgga tctttccgct
      901 cgcgttggat cgcggcatcg gcgtgctcgt ctacctgccg ttcggccgcg atcggctgtg
      961 ggaccgcgtc gaagggcacg cggtgcccga gtgggccgcc gagttcggcg cgacgacgtg
     1021 ggcgcaattc ttcttgaagt ttgcggcttc gcatcccgcc gtgaccgcgg tgacgcccgc
     1081 gacgagcaaa ccgcatcaca tggtcgacaa catgaccgcc gcgcgcggtc ggctgccgga
     1141 tcaagccgag cgcaagcgga tggtcgactt catcgcggcg ctgccgagct aggcagcgcg
     1201 cgccacttct tgggatcgtg caggccgagc gcgaacacca tcccggccgt cacgaacacg
     1261 acgccgacga gctgcagctc gctcggcgtc tcgtgcagca tcggcaccgc gagcagcagc
     1321 gaaaacgccg gcacgagcgc cgcgaacacg gcgccgcgcg cggcgcccag gattgcaacc
     1381 gcgcgcgtat acaagagcaa cgcgacgacg gcggagagca ctccctggaa cacggcttga
     1441 atgacgatct cgcgccacgg cgcggcggcg aggtccgcgc cgccgagcca cgcgtagccc
     1501 ggtaggtaga gcaccatcga cacgacgccg acgatcgccg tcgcgtgcac gggctcgacg
     1561 cgccaagccc gcaagccgat cgtgtagctc gcccagagca aaccgccgag cacgaacatc
     1621 gcatcgccga gccacgtgag ctcgccgtgg ttttgcaggc cgtcccagcc caacgcgacg
     1681 agccccgccg cgatcgcaag cgcgcccgcg atgcggccgc ccgtcagcgg ttctttgagg
     1741 atcagccggc tgccgagcgt cgagcacagc agcatgcagc tcggcgtcat gacgcccatg
     1801 tggccgcccg gcgcgaacgc gagacctccg gcggtcagca gcacgtacgg cgcgccggcg
     1861 ccgcaagcga ggaacagcgc ggtgagcggg ctcaagcttc cgagacggcg gcgcgtgaac
     1921 agcggcagga gcagcaatcc gccgacaccg aagcgcagcg ccgtgatgtc ccacgcgtcg
     1981 agcgcgttgc tcgccatcga cagcttcgtg atcgcaggga agccggccca gatcagggcg
     2041 gcgagcgcgc cgaacagggc gcctgcgata ggcggcgacc gttcgctaat cggcgagccc
     2101 ctgatagacg cggcgctcga aaccttgcag cgtcgcgatt gcgtcgccgt cgtagaacgg
     2161 taggtactgc atcaagagca cgccgccgat gccgcgcgca gggtcgatcc aaaattcggt
     2221 attgaagata ccggcccagc tcatgctgcc cggcgagcgc gacaatttgt cggaatgcgg
     2281 gcccgtgatc tgaaacccga gcccgaactt gtcgcggccc gcgccgagcg gaaacggctc
     2341 cgtcaacgcg ggcagcgcgg ccggctgctg ctcgacgaaa atcgcgccga tgtggttctc
     2401 gcccatcagt gcgacggtcg cctcgctcaa gagccgcttg ccgttcggcg cgcggccgtc
     2461 gttcaggaac agctggatga atttcgcgta gtcggccgcc gtcgaatgca agccgccgtc
     2521 gccgtagacg ggagccgtga tctccgccgg gttgggcgtc tcgacgagcc cctcgggcgt
     2581 cgtgcgatgc acggtcacga cgcggcgggc tttcg
//
LOCUS       DASSNN010000431         2405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1207526, whole genome shotgun sequence.
ACCESSION   DASSNN010000431 DASSNN010000000
VERSION     DASSNN010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207526"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="VFL84_08065"
     CDS             <1..1178
                     /locus_tag="VFL84_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608614.1"
                     /translation="QLGRDLPLYKFAVRDGAGTEVYVSPYVGDVRLVTTTKTRTLAWI
                     ATIPHWFYITPLRTNQPLWYWIVVATSALGCLLALLGLVLGVTQFHKSTPFRWSSVVR
                     YQGLMRWHYISGAFFGIFALTWVFSGLLSMEPFDWANADGLTIRGDALTGGPVELEQF
                     PPFTADAWSALLAGRTLKELEFRRIADAPYYLARYTETGTEMGTDLFSEENGSGPGSA
                     PENRSVPISEPRRERLHQPYPITGRAEPRHMLVDARTLTARTEPFPADTLLERLRAAA
                     PDASIVTHELLADYDSYYYSRNGQAALPVLRVKFDDPLETWVYVDPELGQIVASIHRL
                     HRVERWLYNGLHSLDFGFWYDRRPLWDIGMILLSLGALATSTIGFWLGWKRLRRGLA"
     gene            complement(1159..1908)
                     /locus_tag="VFL84_08070"
     CDS             complement(1159..1908)
                     /locus_tag="VFL84_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HET7608615.1"
                     /translation="MFKRWRRRRILARHSIPDDVWAAALAAWPVLARLTDEQRARLKS
                     LALLFLHEKHFEAARGMALDETKCVRIAAMACAPILELGFDAYRGFRSIVVHPDEFVV
                     RDRTYEDEDGVVHIGDDVLSGEAWEQGPVVLAWSEIEQSGRGEGYNVVVHELVHKLDM
                     LGGEADGVPPLHGDMRVPEWVAAFDAAYEDLCERLERGEEPWLDPYAAEDPAEFFAVC
                     AEMFFDVPRRFRKEYPDVYEQLKRYFKLDLA"
     gene            complement(1991..>2405)
                     /locus_tag="VFL84_08075"
     CDS             complement(1991..>2405)
                     /locus_tag="VFL84_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009022926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7608616.1"
                     /translation="RQDMVNSARYLKAHELSTGKLGATGFCWGGGMVNNLAVALGADF
                     QAGVPFYGAAPAAADVAKIKAPMMFQLAGTDERINAGYPDYEAALKANNVPFEKYVYD
                     GTQHGFHNNSTPRYNEAAANLAWDRTVAFFKRHLA"
BASE COUNT          369 a          826 c          783 g          427 t
ORIGIN      
        1 tgcagctcgg ccgcgacctc ccgctctaca agttcgcggt gcgcgacggc gccggcaccg
       61 aggtctacgt ctcgccttac gtgggcgacg tgcggctcgt cacgacgacg aagacgcgca
      121 cgcttgcctg gatcgcgacg attccgcatt ggttctacat cacgccgctg cgcacgaacc
      181 agccgctctg gtactggatc gtcgtcgcga cgtcggccct cgggtgcttg ctcgcgctgc
      241 tcggcctcgt gctcggcgtc acgcagtttc acaagtcgac gccgtttcgc tggtcgagcg
      301 tcgttcgcta ccaaggctta atgcgctggc actacatctc gggcgccttc ttcggcatct
      361 tcgcgttgac gtgggtgttc agcggcctct tgtcgatgga gcccttcgac tgggcgaatg
      421 ccgacggtct cacgatccgc ggcgacgcgc tgacgggcgg ccccgtcgag ctcgagcagt
      481 tcccgccgtt cacggccgac gcgtggagcg cgctgctcgc cggccgaacg ctgaaggagc
      541 tcgagtttcg ccgcatcgcc gacgcgccct actacctggc ccggtacacg gaaacgggaa
      601 cggaaatggg gacagatcta ttttcggagg aaaatggatc tggccccggt tccgcgcccg
      661 aaaatagatc tgtccccatt tccgagccgc ggcgggagcg gctgcatcaa ccgtatccga
      721 tcacgggccg cgcggagccg cggcacatgc ttgtcgacgc gcgcacgctg accgcgcgca
      781 ccgagccgtt tccggcggac acgttgctcg agcggctgcg cgcggccgcg cccgacgcgt
      841 cgatcgtcac gcacgagctg ctcgcggact acgactccta ctactactcg cgcaacggcc
      901 aggccgcgtt gcccgtgctg cgcgtcaagt tcgacgaccc gctcgagacg tgggtctacg
      961 tcgatcccga gctcggccag atcgtcgcgt cgatccatcg cctgcaccgc gtcgagcgct
     1021 ggctctacaa cggcttgcac agcctcgact tcggcttctg gtacgaccgc aggccgctct
     1081 gggacatcgg catgatcctg ctctcgctcg gcgcgctcgc gacgagcacg atcggcttct
     1141 ggctgggatg gaagcgcctc aggcgaggtc tagcttgaag tagcgcttga gctgctcgta
     1201 gacgtcggga tattccttgc ggaagcggcg cggcacgtcg aagaacatct cggcgcagac
     1261 cgcgaagaac tcggccgggt cctcggccgc gtacgggtcg agccagggct cctcgccgcg
     1321 ctcgagccgc tcgcacaggt cttcgtaggc ggcatcgaac gccgcgaccc actcgggcac
     1381 acgcatgtcg ccgtgcagcg gcggcacgcc gtccgcttcg ccgccgagca tgtcgagctt
     1441 gtgcacgagc tcgtgcacga cgacgttgta cccttcgccg cgcccgctct gctcgatctc
     1501 gctccaggcg agcacgacgg gcccctgctc ccacgcctcg ccggacagca cgtcgtcgcc
     1561 gatgtggacg acgccgtcct cgtcctcgta ggttcgatcg cgcacgacga actcgtcggg
     1621 atgaacgacg atggacctga acccgcggta ggcgtcgaac ccgagctcga gaatcggcgc
     1681 gcacgccatc gcggcaatgc gcacgcattt cgtctcgtcg agcgccatgc cgcgcgccgc
     1741 ctcgaagtgc ttctcgtgca ggaacagcag cgcgagcgat ttgaggcgcg cgcgttgctc
     1801 gtcggtcaat cgcgcaagca ccggccaggc cgcgagcgcg gcggcccata cgtcgtccgg
     1861 tatcgaatgg cgggccagga tgcgccgccg gcgccagcgc ttgaacatga ggtgccggtg
     1921 ttacggtttc ggttcaaccg aagtgccgga gttacggttg aaccgaaccg taacaccggc
     1981 acctcttccg ttaggcgaga tggcgcttga agaatgctac ggtgcggtcc caagcgagat
     2041 tcgccgcagc ctcgttgtag cggggcgtcg agttgttgtg gaacccgtgc tgcgtcccgt
     2101 cgtagacgta tttctcgaag gggacgttgt tggccttcaa cgcggcctcg tagtcggggt
     2161 agcccgcgtt gatgcgctcg tccgtgccgg cgagctggaa catcatcggc gccttgatct
     2221 tcgcgacgtc ggccgccgcg ggtgccgcgc cgtagaacgg cacaccggcc tggaagtcgg
     2281 cgccgagcgc gacggccaaa ttgttgacca tgccgccgcc ccagcaaaaa cccgtcgcgc
     2341 cgagcttgcc cgtcgagagc tcgtgtgcct tcaggtaccg cgcgctgttg accatgtcct
     2401 ggcgc
//
LOCUS       DASSNN010000432         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1207873, whole genome shotgun sequence.
ACCESSION   DASSNN010000432 DASSNN010000000
VERSION     DASSNN010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207873"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFL84_08080"
     CDS             <1..437
                     /locus_tag="VFL84_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608617.1"
                     /translation="ATSIDADLGTQPGTAISTDASGLHLGLGLRRELAQGSIGARLEL
                     DDLDGDLLLAVRALDYGRHVSERFTVTAFAGAARLDLDTPAYGWYLGGGVLLEDLWPR
                     WSLGLDIRFGDKLARDNFDPAAVRPDDFYDLSGVTLYLSRAF"
     gene            complement(416..1483)
                     /locus_tag="VFL84_08085"
     CDS             complement(416..1483)
                     /locus_tag="VFL84_08085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7608618.1"
                     /translation="MRVVLYRPSLEARSGAGRLLAAQWRGLTSAGVPALLACAGGAFK
                     FWLRTGVRARRRSPDDIERLRAHGTIVVDHGLAFPRAALVFVHNLAAEAGRHVPGETA
                     EAAEREREYFRALDPSATVVANSKLVAEALHVHFGLPRERVTVLYPGFDSRRYSLERA
                     AQLRSAARRSLAVGDGVPLVGFVTSGHFAKRGLDLFLECAARIAAARPDARFLVVGSK
                     RLPPDARAHALVRSGAVRHLPKSGRPEPWFAALDLFLYPARFEEFGMVVAEAQAMGVP
                     VLTSRRVGAGECLPESYAPWLLDAPEPEALASRALALLADASLRARLARAAAQSVAAF
                     DERAYADGTRRLIAAQNARLR"
     gene            complement(1508..2719)
                     /locus_tag="VFL84_08090"
     CDS             complement(1508..2719)
                     /locus_tag="VFL84_08090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7608619.1"
                     /translation="MPVVTHLVSLTQAAGVEAHFSEFVRQAHASHPEFAHGWLNAARE
                     MHPYVAERVAGELDHVIHAKRRFGLPLPPRPAALRTWHCRRALAAARTGVLVIWNRTA
                     KAKFALDAIGERRCIHWEHGAAWDAGREAERREYLERVPLAIANSTAAKRVLELLWSY
                     RGDVRVCRNALRPSLVPADGPARKRYPRERAIKLGAAARLYPVKGLAIVLHAVASLRR
                     TLDVELEIAGAGPELARLERLRERLGLRDKVTFHGAVNDMPAFYKSIDLLLHTPITEA
                     FGLVALEAAAHGCPVVAAGVDGLAEAVADGVTGRLVAPTLALARYGELGGVLEGMPQC
                     VYDPAADALREPRAVDPELLAAAVAAQFADARAYEAQSAAASAHVLAGPSFASHVRDV
                     MATIDGFLRGA"
BASE COUNT          510 a         1127 c         1112 g          421 t
ORIGIN      
        1 acgcgacgag catcgacgcc gacctcggca cgcagccggg caccgcgatc tcgaccgacg
       61 cgagcgggct gcatctgggc ttggggcttc gccgcgagct cgcgcaggga agcatcggcg
      121 cgcggctcga gctcgacgat ctcgacggcg atctcctgct cgcggtgcgc gcgctcgact
      181 acggccggca cgtctcggag cgattcacgg tgacggcctt cgcgggcgcg gcgcggctcg
      241 acctcgatac gcccgcgtac ggctggtact tgggcggcgg cgtgctgctc gaggacctct
      301 ggccgcgctg gagcttgggg ctcgacattc gcttcggcga caagctcgcg cgcgacaact
      361 tcgatcccgc ggccgttcgg ccggacgatt tctacgacct ctccggcgtc acgctctacc
      421 tgagccgcgc gttctgagcg gcgatcagcc gccgcgttcc gtcggcgtag gcgcgctcgt
      481 cgaacgccgc gacgctctgc gccgccgcgc gcgcgagccg cgctcgtaag ctcgcatcgg
      541 cgagcagcgc aagagcgcgg ctcgccagcg cctcgggctc cggagcgtcg agcagccacg
      601 gcgcgtagct ctcgggcaag cactcgccgg cgccgacgcg ccgcgacgtg agcacgggga
      661 cgcccatcgc ctgcgcctcc gcgaccacca ttccgaattc ctcgaagcgc gcgggataga
      721 ggaacaagtc gagcgccgcg aaccacggct cgggccggcc gctcttcggc agatgacgca
      781 cggcgcccga acgcacgagc gcgtgcgcgc gcgcatcagg gggcaagcgc ttcgagccga
      841 cgacgagaaa ccgcgcatcg ggccgcgcgg cggcaatccg cgccgcgcat tcgagaaaga
      901 gatcgagacc gcgtttcgcg aaatggcccg acgtcacgaa gccgacgagc ggcacgccgt
      961 cgccgacggc gagcgagcgg cgcgccgccg agcgcagctg cgccgcgcgc tcgagcgaat
     1021 agcgccgcga gtcgaacccg ggataaagca cggtcacgcg ctcgcgaggc aagccgaaat
     1081 gcacgtgcaa cgcctcggcg acgagcttcg agttcgcgac gaccgtcgcg gacggatcga
     1141 gcgcgcgaaa gtattcgcgc tcgcgctccg cggcctccgc cgtctcgccg ggcacgtgcc
     1201 ggccggcttc ggccgcgaga ttgtgcacga acacgagcgc ggcgcgcggg aacgcgaggc
     1261 cgtgatcgac gacgatcgtg ccgtgcgcgc gcagccgctc gatgtcgtcc ggcgagcggc
     1321 gacgcgcgcg cacgccggtc cgcagccaga atttgaacgc gcctcccgcg catgcgagca
     1381 gcgccggcac gccggccgac gtcaagccgc gccactgcgc cgcgagcaac cgccccgctc
     1441 cgctgcgcgc ctcgaggctc ggccgataga gcacaactct catccggcgc ctcggctccg
     1501 tttgcgttta cgcgccgcgc aagaagccat cgatcgtcgc catgacgtcg cgaacgtggg
     1561 aggcgaagct cggccccgcg agcacgtgcg cgctcgcggc cgcgctctgc gcctcgtacg
     1621 cgcgcgcgtc cgcaaattgc gcggcgaccg ccgcggccaa gagctcgggg tcgaccgcgc
     1681 gcggctcgcg caaagcatcc gcggccggat cgtagacgca ctgcggcatt ccctcgagca
     1741 cgccgccgag ctcgccgtag cgcgcgagcg caagcgtcgg cgcaacgagt cgccccgtca
     1801 ctccgtccgc gacggcctcg gcgaggccgt cgacgccggc cgcgacgacg gggcacccgt
     1861 gcgcggcggc ttcgagcgcg acgagcccga acgcctccgt gatcggcgtg tgcagcagca
     1921 ggtcgatgct cttgtagaac gcgggcatgt cgttgaccgc gccgtggaac gtgaccttgt
     1981 cgcgcagtcc caagcgttcg cggaggcgct cgagccgtgc cagctccggt cccgctccgg
     2041 cgatctcgag ctcgacgtcg agcgtgcggc gcaggctcgc gacggcgtgc agcacgatgg
     2101 cgaggccctt caccggatac aggcgcgcgg ccgcgccgag cttgatcgcg cgctcgcgcg
     2161 gatagcgctt gcgtgccggg ccatccgccg gcacgaggct cggccgcagc gcgttgcggc
     2221 agacgcgcac gtcgccgcgg tagctccaca gcaattcgag cacgcgcttc gccgccgtcg
     2281 agttcgcgat cgcgagcggc actcgctcga ggtactcgcg ccgctcggct tcgcggcccg
     2341 cgtcccacgc ggcgccgtgc tcccaatgga tgcaccgccg ttcgccgatc gcgtcgagcg
     2401 cgaacttcgc tttcgcggtg cggttccaga tcacgagcac gcccgtgcgc gccgcggcga
     2461 gcgcgcggcg gcaatgccag gtgcgcagcg ccgcgggcct cggcggcaac ggcaggccga
     2521 aacggcgctt cgcgtggatg acgtgatcga gctcgccggc gacgcgctcg gcgacgtacg
     2581 ggtgcatctc gcgcgcggcg ttgagccagc catgcgcgaa ctcgggatgg ctcgcgtgcg
     2641 cctgccgcac gaactcgctg aaatgcgctt cgacgcccgc ggcctgcgtg agactcacca
     2701 agtgcgtgac gaccggcacg ttaagccgcg cccgcggagc tgaaggaggt ggtgggccgt
     2761 gtaggactcg aacctacaac caatggatta agagtcgacg gctttgaagt cacgagaaca
     2821 ccgagcacga gcgaatccag aggttaagga gacggagccg gtggaaaggt gacgctcgat
     2881 catgtttgtt cagcattctt agtagcgaca tggtagcagc gcgccgggtc aatctccgca
     2941 ggtaggggtg tcaatgacgc caacaagcag ggctctcgat gcagcggctc gagaatctgg
     3001 ctatgatata gccagaaatg cgttacgaga tcgtcctggc cccggaggcc gttgaggatc
     3061 tgaatgcgct caaggccgct acgaaggcct ccgtcacgga agcgctcgaa acgcatctac
     3121 gccatgagcc aaccaaagtg agcaagagcc ggatcagacg cctgcgaggc
//
LOCUS       DASSNN010000433         3337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_206412, whole genome shotgun sequence.
ACCESSION   DASSNN010000433 DASSNN010000000
VERSION     DASSNN010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206412"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFL84_08095"
     CDS             complement(<1..463)
                     /locus_tag="VFL84_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET7608620.1"
                     /translation="MKHWDRRSVLTALGSVGASLWLPPSADALGLPDDKIKRVRYFSN
                     SGDAQGRRGQPMVNQSTNVVMIETERGLVGIGEGGEPTSMDECASMLIGQDPFRIDQH
                     WQRMLRGYFYPAGREKLHSLGALDVALWDLKAKALDVPVWQLLGGKSRDHLE"
     gene            complement(480..1424)
                     /locus_tag="VFL84_08100"
     CDS             complement(480..1424)
                     /locus_tag="VFL84_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7608621.1"
                     /translation="MGLKAQPSASGTPAELVAAAKGAAGRDHSGTFLRICVAPDNLNA
                     RRPPPLTGAAVVPDWSTWYAAPYKLFDNLYFLGTQIHSSWALTTTDGIVVIDTLFDYA
                     IEPEIVAGLTTLGLNPRDIRYVLISHAHGDHDQGAALLQSRYGAKVVMGAADWEATLQ
                     RPADARGGVPKRDIAVGPEGLRLTLGDITIDVIATPGHTPGTLSYIFPVRDRGRTMTV
                     AYAGGTAFNFPREANNFAIYHDSQKKMLEAARAAGATVLMTNHTEFDRAYDRVRLAQL
                     PRAAGEKHPYETDAATVQRYFEMGGLCAEAQRLSMSSN"
     gene            1607..2878
                     /locus_tag="VFL84_08105"
     CDS             1607..2878
                     /locus_tag="VFL84_08105"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7608622.1"
                     /translation="MAERLRITNVCAGLSLGLLLAGVTLEVEADGSAVIAGMSPERLQ
                     RLDEHFHRYVDDGLISGVVTYIVRGAEVVHQDAYGLADISARRPMSQDTYFYLYSMTK
                     PVTSVALLMLYEEGKFQLDDPIAKYLPELAGLKLYVGEGNDGKMIVKDPARQPTVHDV
                     FRHTAGFLYGGNGDRGIDRAYSEADLMNGTLAELTRKLGKIPLAYEPGTQWVYSVSHD
                     VQARLVEVLSGMPFDEFAQRRIFAPLGMKNTVFGRPGSLKDRFAVIYGGSQRGQLQPT
                     GALDRPGAASRVLGGLSLSSTAADYARFAEMLVNSGELDGVRILSRKTVDLMDSNHLP
                     HGVTRGAAGGGTAGGEGYGLGVRVVTDPAAAGNLTSEGTFGWSGAAGTHFFVDRKEDL
                     VAVFMIQSMGGAGGPRMSDEFETLVYQAIVD"
     gene            2887..>3337
                     /locus_tag="VFL84_08110"
     CDS             2887..>3337
                     /locus_tag="VFL84_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HET7608623.1"
                     /translation="MSRAIDRRRFLARLGSAGALLAGGSWLPGFGYAQARGPARAVID
                     QRHVRADLDRRVLGAFLEHLGRAVYGGVYDPDNSLSDSRGFRRDVMDEIRQMGVPIIR
                     YPGGNFVSGYNWQDGVGPKSERPTVLERAWNSLEPNQFGTNEFIDWCL"
BASE COUNT          521 a         1110 c         1126 g          580 t
ORIGIN      
        1 gctcgagatg gtcgcgcgac ttgccgccga gcagctgcca cacgggcaca tcgagcgcct
       61 tcgccttgag gtcccacagc gcgacgtcga gcgcgccgag cgagtgcagc ttttcgcggc
      121 cggccggata aaagtagccg cgcagcatgc gctgccaatg ctgatcgata cggaacggat
      181 cctggccgat cagcatgctc gcgcattcgt ccatcgacgt cggctcgccg ccctcgccga
      241 tgccgacgag cccgcgttcg gtctcgatca tgacgacgtt cgtcgactgg ttgaccatcg
      301 gctgcccgcg gcggccctgc gcatcgccgg agttgctgaa atagcgcacg cgcttgatct
      361 tgtcgtcggg aaggccgagc gcgtcggccg acggcggtag ccacaggctc gcgccgacgc
      421 tgccgagcgc ggtcagaacg ctacgtcgat cccagtgctt catgacgccg ctccgtcaat
      481 cagttcgaag acatcgacag gcgttgcgct tcggcgcaaa ggccgcccat ctcgaaatag
      541 cgctgcaccg tggccgcgtc ggtttcatag ggatgctttt cgccggccgc gcgcggcagc
      601 tgcgcgaggc gcactcggtc ataggcgcgg tcgaactccg tgtggttggt catcagcacc
      661 gtggcgccgg cggcgcgcgc ggcctcgagc attttctttt ggctgtcgtg gtagatcgcg
      721 aagttgttgg cttcacgcgg gaagttgaaa gccgtgccgc cggcgtacgc gactgtcatc
      781 gtccggccgc ggtcgcggac cgggaagatg tacgacagcg tgccgggcgt atggcccggc
      841 gtggcgatga cgtcgatcgt gatgtcgccg agcgtgagcc gcaaaccttc gggccccacc
      901 gcgatgtcgc gtttcggcac gccgccccgg gcgtccgcgg ggcgctgcaa cgtcgcctcc
      961 caatcggccg ctcccatgac gaccttggcg ccgtaccggc tctgcagcaa cgcggcgcct
     1021 tgatcgtgat cgccatgcgc gtggctgatt agaacgtacc tgatgtcgcg cggattgagg
     1081 cccagcgtcg tgaggccggc cacgatctcg ggctcgatcg cgtaatcgaa cagcgtgtcg
     1141 atcacgacga tgccgtccgt cgtcgtcaac gcccacgacg agtggatttg cgtgccgagg
     1201 aagtacaggt tgtcgaacag cttgtacggc gcggcgtacc acgtcgacca gtccggcacg
     1261 acggcggcgc ccgtgagcgg cggcgggcgc cgcgcgttca gattgtcggg cgcaacgcag
     1321 atccgcaaaa aggtcccgga gtgatcgcgg ccggcggctc ccttcgccgc ggccacgagc
     1381 tctgcgggcg tgccggacgc cgacggctgg gcctttagac ccatggcggc tgacgcggcg
     1441 aacgcgccaa ccgcgaaaag gcaatatcgc ataacatttc cccctcggtg cgcatcatgc
     1501 cactcgtcgc ggcggttgtg tgcacgcgca gcgcctgcgc ggcccgcgca cggatgggca
     1561 atgatgatat ggtctgcagc gcgtaggact aacaaggggg atcatcatgg ctgaacggct
     1621 tcggatcacg aacgtctgcg ccggtttatc gctcggtctt ctcttggccg gcgtaaccct
     1681 cgaggtcgag gcggacggct cggccgtcat cgccggcatg tcgccggaac ggctgcagcg
     1741 cctcgacgag cattttcacc gctacgtgga cgacggcctc atttccggcg tggtgacgta
     1801 catcgtgcgc ggcgctgaag tcgtgcacca ggatgcgtac ggcctcgccg acatctcggc
     1861 ccggcggccc atgtcgcagg acacgtactt ctacctctac tcgatgacga agcccgtcac
     1921 gagcgtggcg ctgctgatgc tctacgagga aggcaagttc cagctcgatg atccgatcgc
     1981 caagtatctg ccggagctcg ccggtctcaa gctctatgtc ggcgaaggca acgacggcaa
     2041 gatgatcgtc aaagatcccg cgcgtcagcc gacggtccat gacgtgttcc gccatacggc
     2101 cggtttcctg tacggcggta acggcgatcg cggcatcgac cgcgcatact ccgaagcgga
     2161 cctcatgaat ggcacgctgg ccgagctcac gcgcaagctc ggcaagatac cgctcgctta
     2221 tgagcccggc acgcaatggg tgtattcggt atcacacgac gtgcaggctc gcctcgtcga
     2281 ggtgctgtcc ggcatgcctt tcgacgagtt cgcgcagcgg cgcattttcg ctccgctcgg
     2341 catgaagaac accgtgttcg gccggcccgg ctcgctcaag gatcggttcg ccgtcatcta
     2401 cggcgggagc cagcgcgggc aactgcagcc gaccggggcg ctcgatcggc cgggcgccgc
     2461 gagccgcgtg ctcggcggct tgagcctgtc gtcgacggct gccgattacg cgcggtttgc
     2521 ggagatgctc gtgaattccg gcgagctcga cggcgtgcgc atcctgtcgc gcaagaccgt
     2581 cgacctcatg gattcgaacc acctgccgca cggcgtcacg cgcggtgcgg ccggcggcgg
     2641 cacggccggc ggcgagggtt acggtctcgg cgtgcgcgtg gtgaccgatc cggccgcggc
     2701 cggcaacctg acgtcggagg gcacgttcgg ctggtcgggc gccgccggca cgcacttctt
     2761 cgtcgatcgc aaggaagacc tcgtggccgt gttcatgatc cagagcatgg ggggcgccgg
     2821 cgggcctcgc atgtcggacg agttcgaaac gctcgtctat caagccatcg tggattgacc
     2881 aagccgatga gccgcgcgat cgatcgacgc cggttcctcg cgcgcctcgg ctccgcgggc
     2941 gccttgctcg ccggcggctc ctggcttccg ggcttcggct acgcgcaagc gcgcggcccg
     3001 gcgcgcgccg tcatcgatca acggcacgtc cgtgccgatc tcgaccgccg cgtgctcggc
     3061 gctttcctcg agcatctcgg gcgcgccgta tacggcggcg tctacgatcc ggacaactcc
     3121 ctctccgact cgcgcggctt ccgccgcgac gtcatggacg agattcggca gatgggcgtg
     3181 ccgatcatcc gttatccggg cggcaacttc gtctcgggtt acaactggca ggacggcgtc
     3241 ggcccgaaga gcgagcggcc gacggtgctc gagcgcgcgt ggaactcgct cgagccgaat
     3301 cagttcggca cgaacgagtt catcgactgg tgcctgg
//
LOCUS       DASSNN010000434         5600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1689836, whole genome shotgun sequence.
ACCESSION   DASSNN010000434 DASSNN010000000
VERSION     DASSNN010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689836"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFL84_08115"
     CDS             complement(<1..894)
                     /locus_tag="VFL84_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7608624.1"
                     /translation="MRTGQRGTRPALAIWAAFVTLLGVAGCSREQEPAPAPAAPPPAA
                     TPAPAPAAAATIRDSADPLEHGRYLVETVAGCGNCHTPRLPDGTPDATKNLAGAFVIE
                     EPIFKAYARNITPDMETGIGSWTEDQIVDAIRNARRPDGTWLGPPMSFGWYKGMSDTD
                     AHAIAKYIKTVPAVRNEVPKSTYMIPGGPPENNIYGPIVTSVPDVPKTDIVKYGQYLA
                     GPVGHCMDCHTTYVMGQIDMTQLGRGGNVYSKPFIYDWAAVSANITSHPTAGLGSWTD
                     AEIKHAITEGESRDGRKLLPFM"
     gene            1187..2515
                     /locus_tag="VFL84_08120"
     CDS             1187..2515
                     /locus_tag="VFL84_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608625.1"
                     /translation="MAVDPEILREQAASIVRSGALGRSRSYARLLEFLVECARCGRTP
                     KELEIAMEVFGRGADFDPSQDSMVRVYAHNLRQKLEHYYATAGRNEPRQLVLARGEYR
                     ISVAETAEPAGSADAATPAQLAAPLPLPAAAPDLPKAAVSRWRLAGVGAALLVVGLAL
                     GAGIVIESEPAAPASAAVAKSPLWADMLDDDLPILVVVGDYYIYGELNDYGEVTRLVR
                     DFNVGSSAELDELMKNDTTLMARYMNLDLTYLPTGSAFALLDLLRVIYTTNKSVRVVS
                     MSEMNEADLKSSHVIYVGYISALDKLEDFVFASSTLAIGYTYDELKNTDTGETYTSEA
                     GYPEINHNYRDYAFISTFPGPGGNQLMIVSGTRDAGLMQAAHALADPMFVRSMEQQRP
                     DAGKNQPPSFEMLYEVTGYGRTNLDAMLVHTAPLNYQQIWGGVLRQPPAD"
     gene            complement(2497..3540)
                     /locus_tag="VFL84_08125"
     CDS             complement(2497..3540)
                     /locus_tag="VFL84_08125"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HET7608626.1"
                     /translation="MSERQAARAVLMVRPAQFGSNAETAGSNFFQRPATAMDGGSAAN
                     AGEGMDARGRATQGAVAGSNSRPHAAGEAARRAQHEFDALALALAGAGVRVLEFEGQR
                     GAGLPDEVFPNNWLSLHADGTAVLYPMLAPSRRRERRRDILDALVDSYGYRIDRVVDL
                     TSLEARGEYLEGTGSVALDRVAHVAYACLSPRTHLRALGELARALHYDVVPFTAVDSQ
                     RRPIYHTNVMLSVGTRFAALCSAAVADTAERRNVVARLEESGREIIDLSPEELEGFAC
                     NLLELDGAGGPVIALSAAALRALAAPTRGALERHGQLVMADIATIERLGGGSVRCMLA
                     EVALPRRDQSAGG"
     gene            complement(3537..4493)
                     /locus_tag="VFL84_08130"
     CDS             complement(3537..4493)
                     /locus_tag="VFL84_08130"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HET7608627.1"
                     /translation="MTSSARSRAAPLTAALALFGCASAPQLPLDSSVEELMAVPFFPQ
                     TEYQCGPAALATVLKHAGADVDADALAPQVYAPGLRGSLQPELLGATRRHGFVPYVIE
                     PDVRALAAELAARRPVLVLQNLSVPRVPVWHYAVVVGLAGNEVVLRSGTQERRIERGA
                     RFLREWQRGANWGFVALAPGELPATENAGAYVRALADAEPVLGAAAAERSYRAALERW
                     PNDEFVLFASAGQRYAAGDLTEATMLYRRLLASAPQNAAARNNLANVLAERGCHAEAL
                     AEAHTALAAVPPSDELGAAVRDTVAELERAAAQGQAPAVQCP"
     gene            complement(4471..4848)
                     /locus_tag="VFL84_08135"
     CDS             complement(4471..4848)
                     /locus_tag="VFL84_08135"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HET7608628.1"
                     /translation="MRNHRHSLVAVLAVSVFSSSLQSAAYAGVISTQQYLTTLDREAT
                     RARIDAVLAREEVRSKLEQYGVDPAMTDARLAALTDQELEQLATDLENMPAGGSVLGV
                     IGVVFIVLLILELTGVIDIFSKI"
     gene            complement(4902..5135)
                     /locus_tag="VFL84_08140"
     CDS             complement(4902..5135)
                     /locus_tag="VFL84_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HET7608629.1"
                     /translation="MAKNRPTEERLDELREAFDYNDRDGDGRIQLDEFSAMLDELDSE
                     MSRREIEIGFKDIDTNDDGLIDFDEFVGWWSES"
     gene            complement(5159..>5600)
                     /locus_tag="VFL84_08145"
     CDS             complement(5159..>5600)
                     /locus_tag="VFL84_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608630.1"
                     /translation="AGNLLYELVPQTRRAAYVAFHNVGTAAGVFGGAMLGSLLQVVLP
                     PGTVLFGGSGAPSSLLYVFVISGIARAIVAALLARRVRELRKPRRALSAPALVLRVTG
                     LNAMVGVIYDFIGRPLLEDDDRDEPAGQPRRAAQPKKGDNGARD"
BASE COUNT          879 a         1886 c         1928 g          907 t
ORIGIN      
        1 catgaacggc agcaacttgc ggccgtcgcg gctttcgcct tccgtgatcg cgtgcttgat
       61 ctcggcgtcg gtccagctgc cgaggccggc cgtcggatgc gacgtgatgt tcgccgagac
      121 ggcggcccag tcgtagatga acggcttcga gtagacgttg ccgccgcggc cgagctgcgt
      181 catgtcgatc tggcccatga cgtacgtcgt gtggcaatcc atgcagtggc cgacgggacc
      241 cgcgagatac tgaccgtact tgacgatgtc ggtcttcggc acgtcgggca cgctcgtgac
      301 gatcgggccg tagatgttgt tctcgggcgg tccgcccgga atcatgtagg tgctcttcgg
      361 cacctcgttg cgcacggccg gcaccgtctt gatgtacttc gcgatcgcgt gcgcgtcggt
      421 gtcggacatg cccttgtacc agccgaacga catcggcggg ccgagccacg tgccgtcggg
      481 cctgcgcgcg ttgcggatcg catcgacgat ctggtcttcg gtccacgagc cgatgcccgt
      541 ctccatgtcc ggcgtgatgt tgcgcgcata cgccttgaag atcggttcct cgatcacgaa
      601 cgcgccggcg agattcttcg tcgcatcggg cgtgccgtcg ggcaaacgcg gcgtatggca
      661 gttgccgcag ccggcgaccg tttcgacgag gtagcggccg tgctcgagcg gatccgcgct
      721 gtcgcgaatc gtcgcggcgg ccgcgggcgc aggcgcggga gtggcggcgg gcggtggagc
      781 cgcaggtgcg ggcgcgggct cttgctcgcg ggagcagccc gcgaccccca gtagcgttac
      841 gaatgccgcc cagatcgcga gggcagggcg cgttccccgt tggccggttc tcacctgtcg
      901 ttctcctttg tagtggcgcg cagaaacaag gcggcaagca tacgaaaaaa ccggcgccgt
      961 tcggaagaag tattgccgtt gcagattagc cgcggcgggc ggttacgcgg cccccgcgaa
     1021 cgaggcgcga gcgcggctcc ggcgcgcccg ctgcgccgcc gcgctggccg gattatggtt
     1081 tatccagaca aaaccacagc ttaggagtgg tttattaacg gttaaggatc ggttagtttg
     1141 ggcgtctcga aacgacgagc gccaagcgtc ctcgagacgc gaattgatgg ccgtagaccc
     1201 cgaaatcctg cgagaacaag cggcgagcat cgtccgcagc ggtgcgctcg gccgctcgcg
     1261 ctcgtacgcg cgcttgctcg aattcctcgt cgagtgcgcg cgctgcggcc gcacgccgaa
     1321 ggagctcgag atcgcgatgg aagtattcgg ccgcggcgcc gacttcgatc cgagccaaga
     1381 ttccatggtg cgcgtctacg cgcacaacct gcgccagaag ctcgagcatt actacgcgac
     1441 ggcggggcgc aacgagccgc gtcagctggt gctcgcgcgc ggcgagtatc gcatctcggt
     1501 cgccgagacc gcggagccgg ccggctcggc cgacgctgcg accccggcac aactcgccgc
     1561 gccactgcct ttgcctgccg cggcgcccga cctaccgaaa gccgcggtct cgcgctggcg
     1621 cctcgccggt gtcggtgcgg cgctgctcgt ggtcggcttg gcgctcggcg cgggaatcgt
     1681 catcgagagc gagccggccg cgccggcctc ggcagccgtc gcgaagtcgc cgctctgggc
     1741 cgacatgctc gacgacgatt tgccgatctt ggtcgtggtc ggcgattact acatctacgg
     1801 cgagctcaac gattacggcg aggtgactcg gctcgtgcgc gacttcaacg tcggctcgag
     1861 cgcggagctc gacgagctca tgaagaacga cacgacgctg atggcgcgtt acatgaatct
     1921 cgatctcacc tatttgccga ccggcagcgc gtttgcgttg ctcgatctgt tacgagtgat
     1981 ctatacgacc aacaagtcgg tgcgcgtcgt gtcgatgtcg gagatgaacg aggcggatct
     2041 caagtcgagc cacgtcatct acgtcggcta catcagcgcg ctcgacaagc tcgaagactt
     2101 cgtcttcgcg tcttccacgc ttgcgatcgg ttacacgtac gacgagctga agaatacgga
     2161 cacgggcgag acctacacga gtgaagccgg ataccccgag atcaaccaca actaccgcga
     2221 ctacgcgttc atctcgacgt tcccgggccc cggcggcaac cagctcatga tcgtcagcgg
     2281 cacgcgagac gcgggcctca tgcaggccgc gcacgcgctc gccgatccga tgttcgtgcg
     2341 gagcatggag cagcaaaggc cggacgccgg caagaatcag ccgccgtcgt tcgagatgct
     2401 ctacgaggtc acgggctacg gccgcacgaa cttggacgcc atgctcgtcc acaccgcccc
     2461 cttgaactac cagcagatct ggggcggcgt gctgcgtcaa ccgccggccg actgatctcg
     2521 gcgcggcaag gccacttcgg ccagcatgca gcgcacgctg ccgccgccca agcgctcgat
     2581 cgttgcgatg tcggccatga ccaattggcc atgacgctcg agcgcgccgc gtgtcggcgc
     2641 ggcaagcgcg cgtagcgccg cggccgagag cgcgatgacc gggccgcccg cgccgtcgag
     2701 ctcgagcaag ttgcatgcga aaccttcgag ctcctccggc gagaggtcga tgatctcgcg
     2761 tcccgactcc tcgagccgcg cgacgacgtt gcgccgctcg gcggtatcgg caactgcggc
     2821 cgagcagagc gccgcaaagc gcgtgccgac cgagagcatg acgttcgtgt gatagatcgg
     2881 ccggcgttgc gagtcgacgg ccgtgaacgg cacgacgtcg tagtgcagcg cgcgcgcaag
     2941 ctcgccgaga gcgcgaaggt gcgtgcgcgg cgatagacac gcataggcga catgcgcgac
     3001 gcggtcgagc gcgacgctgc ccgtgccttc gaggtattcg ccgcgcgcct cgaggctcgt
     3061 gaggtcgacg acgcggtcga tgcggtagcc gtacgagtcg acgagcgcgt cgaggatgtc
     3121 gcggcgccgc tcgcggcgcc ggctcggcgc cagcatcggg tacagcacgg ccgtgccatc
     3181 ggcgtgcagg ctgagccagt tgttgggaaa cacttcgtcg ggtagaccgg cgccgcgttg
     3241 gccctcgaac tcgagcacgc gcacgcccgc gccggcgagc gcgagggcga gagcgtcgaa
     3301 ctcgtgctgc gcgcgccgcg cggcttcgcc tgcggcgtgc ggccgcgaat tgctcccggc
     3361 cactgctccc tgcgttgccc tacctcgggc atccatgccc tcgcctgcat tcgcggcact
     3421 tccgccatcc atggcggtcg ccgggcgctg gaagaagttc gagcccgcag tctcggcgtt
     3481 gctgccgaac tgcgcgggac gaaccatcag caccgcgcgg gccgcctgcc gctcgctcac
     3541 gggcactgca ccgccggcgc ttggccttgc gctgccgcgc gctcgagctc ggcgaccgtg
     3601 tcgcgcacgg cggcgccgag ctcgtccgag ggcggcaccg ccgccagtgc cgtatgcgct
     3661 tccgcgagcg cttccgcgtg gcagccgcgc tcggcgagaa cgttggcgag gttgttccgc
     3721 gccgctgcgt tttgcggcgc gctcgcgagc agccgtcgat agagcatggt cgcctcggtg
     3781 aggtcgcccg cggcgtaccg ttgcccggcc gacgcgaaca gcacgaactc gtcattcggc
     3841 cagcgctcga gcgcggcgcg atagctgcgc tcggccgccg cggcgccgag cacgggctcg
     3901 gcgtcggcga gcgcgcggac atacgcgccg gcattctcgg tggccggcag ctcgcccggc
     3961 gccaacgcga cgaatcccca gttcgcgccg cgctgccatt cgcgcaggaa gcgcgcgccg
     4021 cgctcgatgc gccgctcttg cgtgcccgag cgcagcacga cctcgttgcc ggccaggccg
     4081 acgacgacgg cgtagtgcca tacgggcacg cgcgggacgc tcaagttctg cagcaccaaa
     4141 acgggccgcc gcgccgcgag ctccgccgcg agcgcgcgca cgtcgggctc gatgacgtac
     4201 gggacgaagc cgtgccggcg agtcgcgccg agcagctccg gttgcaagct gccgcgcagg
     4261 ccgggcgcgt acacctgcgg cgcgagcgcg tccgcgtcga cgtcggcgcc cgcatgtttc
     4321 agtaccgtcg cgagcgcggc ggggccgcac tggtattcgg tctgcgggaa gaacgggacg
     4381 gccatcagct cttcaacgct cgagtcgagc ggcagctgtg gagcgctcgc gcagccgaac
     4441 agcgcgagcg cggccgtgag cggcgccgcc ctagatcttg ctgaagatgt caatgacgcc
     4501 cgtgagctcg agaatcagca acacgatgaa cacgaccccg atgaccccga gcacgctgcc
     4561 gccggccggc atgttctcga ggtccgtcgc gagctgctcg agctcttggt cggtcaatgc
     4621 cgcgaggcgc gcgtcggtca tggccgggtc gacaccatac tgctcgagct tgcttcgcac
     4681 ctcctcgcga gcaagcaccg catcgatgcg cgcgcgcgtc gcctcgcggt cgagcgtcgt
     4741 caggtactgc tgcgtgctga tgacgccggc gtaggcggcg ctttgcagcg acgatgaaaa
     4801 aaccgagacc gcgagaaccg cgacgagcga gtgacgatgg ttgcgcattg agctaactcc
     4861 ctgtccgctt gggtcgaaag tcgagtcgtt gggtccgacg gttaggactc ggaccaccag
     4921 ccgacgaact cgtcgaaatc gatgagcccg tcgtcgttcg tgtcgatgtc cttgaagccg
     4981 atctcgatct cgcggcgcga catttccgaa tcgagctcgt cgagcatggc ggagaactcg
     5041 tcgagctgta tgcgtccatc gccgtcgcga tcgttgtagt cgaatgcttc gcgcagctcg
     5101 tcgagccgtt cttcggtggg tcggttcttc gccattgaat gccgctcctc gggcaactct
     5161 agtctcgggc gccattgtcg ccttttttcg gctgcgctgc tcgccgcggc tggcccgccg
     5221 gctcgtcgcg gtcgtcgtcc tcgagcaggg gtcgtccgat gaagtcgtag attacgccga
     5281 ccatggcatt caaacctgtg acgcgcagca cgagcgcggg cgccgacagc gcgcggcgcg
     5341 gcttgcgcag ctcgcgaacg cggcgtgcca gcagcgccgc gacgatcgcg cgtgcgatgc
     5401 cggaaatcac gaagacatag agcaagctgc tgggtgcgcc ggagccgccg aacagcaccg
     5461 tgcccggcgg cagcacgacc tggagcagcg agccgagcat ggcgccgccg aacacgccgg
     5521 cggcggtgcc gacgttgtgg aacgcgacgt aggccgcgcg ccgcgtttgc ggcacgagct
     5581 cgtagagcaa gttaccggcc
//
LOCUS       DASSNN010000435         4282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_206810, whole genome shotgun sequence.
ACCESSION   DASSNN010000435 DASSNN010000000
VERSION     DASSNN010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206810"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFL84_08150"
     CDS             complement(<1..470)
                     /locus_tag="VFL84_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HET7608631.1"
                     /translation="MSDVPGFAPDWPAPRNVRAWVTERGSAARYGALNLALHVGDDAS
                     AVAANRARLTAALELRSEPRWLTQVHGVRVIDLDRDDGSEADGAVTRRAGVVCAVMTA
                     DCLPVLLCDRAGTRVGVAHAGWRGLLNGVLPAAVAACGDANELIAWLGPAIGQSA"
     gene            complement(467..1423)
                     /gene="rluD"
                     /locus_tag="VFL84_08155"
     CDS             complement(467..1423)
                     /gene="rluD"
                     /locus_tag="VFL84_08155"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HET7608632.1"
                     /translation="MTRIVKRLSVPPELAGRRLDQAAAQLLPEFSRSRLRAWIDAGAL
                     LVGGREAKARTLVKGGEELALQAELEAAVEARPEPIPLSVVHADDALLVVDKPVGLVV
                     HPGAGNRSGTLQNALLHRYPELASLPRAGLVHRLDKDTSGLLLVARTLPSHTALTAAL
                     ERREIKRTYRAICQGVLTGGGTVDAPIGRHRRERTKMAVTESGRAARTRYRVVERFRA
                     HTHCELELETGRTHQIRVHMAHIRAPLLGDPVYGGRPKLPPAASDELRAALQGFRRQA
                     LHAARLRLAHPITGNELDLESPIAADIAALLELLRADASAPR"
     gene            1530..2285
                     /locus_tag="VFL84_08160"
     CDS             1530..2285
                     /locus_tag="VFL84_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET7608633.1"
                     /translation="MLRIGKYSLLLVLVAAAVAGCNRSNVVEDQGPELLYQKGYTAMD
                     ASNYAGAIEYFNALAARYPFSPETRQAQLDLIYLYYRSLQPELAIDAAEQFERENPTH
                     ERLDYCLYMRGLVYFDQAPNVIEKLFRVDLSLRPPKDTLKSFSAFQELIRRFPDSEYV
                     PDARQRMVFLRNRLAQYENHVADYYLRRGAPVAAINRAKYALEHYPEAPELEMTLQLL
                     VAAYEQLGMTDLAADARRVLRESFGEEAATARL"
     gene            complement(2286..4088)
                     /locus_tag="VFL84_08165"
     CDS             complement(2286..4088)
                     /locus_tag="VFL84_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608634.1"
                     /translation="MTPAFRYALVWIFGSLVLVVAALTMVAGARVDGKYVPSSPDAFY
                     HARRILDVVMAGEPVRQFDASIHYPDGSWVPWPWGFDTAMATIVGWFGPFAAESDAAA
                     VLLHLPVAAAVLAVGLLVLLWRQLDLSLTAAALATLAFAVLPMVFLAFAVGNVDHHFA
                     ELICALLAACSGIAFFRSTASVVPAVLLGVVLGLAVAVQNGLFILQLPVVLALAWRWL
                     RREPLPPRRAVLALAAALVTTTLLVSIPSEPWRGGVFEFYSLSWFHSYVAAATAVVSI
                     ALAIVRPRLASALGLALAAAAVAVPLLRSAGLGAQFMSGDLAVLRDITEARSPYALYA
                     LFGPAQSTRLFSWLMWLAPLALLANLYWAVRANEPGRQFFAVAAAMFLVLMQLQYRFG
                     VLGVVPLLATLALALDACSKRWPAWIAAQRIASAVVLVAALTPTAAVWSTPRPAGGDP
                     FYADMYPGLMQLREACERRPGVVLAGINDGHWIRYHTRCSVIGNVFLLTEQDARKRLE
                     VETLLATEPARLRHDRADVAYVLVHRELELFVPVLADGRHGQGVLRWRTDTLPTLVRE
                     LLGPEESLPEGYGALWSAYLPDGQALGRLLEIER"
BASE COUNT          762 a         1492 c         1444 g          584 t
ORIGIN      
        1 gcggattgcc cgatcgcggg accgagccag gcgatgagct cgttcgcgtc gccgcaagcg
       61 gccaccgcgg ccgggagcac gccgttcaag aggccgcgcc agccggcatg cgcgacgccc
      121 acgcgcgtac cggcccgatc gcacaacaac acgggcaagc agtcggccgt catgacggcg
      181 cagaccacgc cggcgcggcg cgtgaccgcg ccgtcggctt cgcttccgtc gtcgcgatcg
      241 agatcgatca cgcgcacgcc atgaacttgc gtgagccaac gcggctcgga gcgcagctcg
      301 agcgcagcgg tcaggcgcgc gcgattggcg gcaacggcgc tcgcgtcgtc gccgacgtgc
      361 agcgcgagat tcagcgcgcc gtagcgcgcg gcggagccgc gctccgtgac ccaagcccgc
      421 acgttgcgcg gcgcgggcca gtccggcgca aaccccggaa cgtcgctcat cgcggcgcgc
      481 tcgcgtccgc gcgcaacagc tcgagcagcg cggctatgtc ggccgcgata gggctttcga
      541 ggtcgagctc gtttccggtg atcgggtgcg cgagccgcaa acgagccgcg tgcagcgcct
      601 gcctgcggaa tccctgcaac gcggcgcgca gctcatcgct cgcggcggga ggcagcttcg
      661 gcctgccgcc gtagaccgga tcgccgagca acggcgcgcg gatgtgagcc atgtgcacgc
      721 ggatctggtg cgtgcgcccc gtctcgagct cgagctcgca atgggtatgc gcgcggaacc
      781 gctcgacgac gcggtagcgc gtgcgggccg cccgcccgct ctcggtcacg gccatcttcg
      841 tgcgctcgcg ccgatgccgg ccgatcggcg cgtcgacggt gccgccgccg gtcagcacgc
      901 cttggcagat cgcgcggtaa gtgcgcttga tctcgcgccg ctcgagcgcc gcggtcaacg
      961 ccgtgtggct cggcagcgtg cgcgcgacga gcaggaggcc gctcgtgtcc ttgtcgagcc
     1021 ggtgcacgag acccgctcgc ggcaacgaag cgagctcggg atagcggtgc agcaacgcgt
     1081 tctgcagcgt tcccgagcga ttccccgcgc ccgggtgcac gacgaggccg acgggcttgt
     1141 cgacgacgag caacgcgtcg tcggcgtgaa cgacgctgag cgggatcggc tcgggccgcg
     1201 cttcgacggc agcctcgagc tcggcttgca gcgccagctc ctcgccgcct ttgacgagcg
     1261 tgcgggcttt ggcctcgcgg ccgccgacca gcagcgcccc ggcatcgatc catgcgcgga
     1321 gccggctgcg cgagaactcg ggcagcagct gcgcggcggc ttgatccagg cggcggccgg
     1381 ctagctccgg gggcaccgat aagcgtttta caatgcgggt cacggccctt ccgaccttca
     1441 ggctggaaac tttagctata ctgcgccgtc gaaacagcca cgactgattg gcggcattat
     1501 tgccgctcct cgagctccgg acatagcacg tgcttcgaat cggcaaatat tcactgctgc
     1561 tcgttctcgt tgcggcggcc gtcgcgggtt gcaaccgcag caacgtcgtc gaggaccagg
     1621 gccccgagct cctctaccag aagggctaca cggccatgga cgcctcgaac tacgccgggg
     1681 ccatcgagta cttcaatgcg ctcgccgcgc gctatccgtt cagccccgag actcggcagg
     1741 cgcagctcga tctcatttat ctttattacc gcagcctgca gcccgagctc gccatcgacg
     1801 ccgcggagca gttcgagcgc gaaaatccga cgcacgagcg cctcgattac tgcctctaca
     1861 tgcgcggtct cgtgtatttc gaccaggcgc cgaacgtcat cgagaagctg ttccgggtcg
     1921 atctatcgct gcggcccccg aaggacacgc tgaagtcctt ctcggcgttt caggagctga
     1981 tccgccgctt tccggatagc gagtacgtcc ccgatgcgcg ccagcggatg gtgttcctgc
     2041 gcaatcggct cgcccagtac gagaaccacg tcgccgacta ctacctgcgc cgcggcgcgc
     2101 ccgtggccgc gatcaaccgc gccaaatacg cgctggagca ctatcccgaa gcgcccgagc
     2161 tcgagatgac gctgcagctg ctcgtcgccg cttacgagca gctcggcatg acggatctcg
     2221 cggccgacgc gcggcgcgtg ctgcgcgagt cgttcggcga agaagccgcc acggcgcgcc
     2281 tctagctagc gctcaatctc gagcagcctt cctaacgcct gcccgtccgg caggtaggcc
     2341 gaccagagcg ctccgtagcc ctcgggcagc gattcctccg gtccgagcag ctcgcgaacg
     2401 agagtcggca acgtatcggt tcgccagcgc aacacgcctt gtccgtggcg accgtcggcc
     2461 aagaccggca cgaacagctc gagctcccga tggacgagca cgtaggcgac gtccgctcgg
     2521 tcatggcgca agcgcgcggg ttccgtcgcc agcagcgttt cgacctcgag ccgcttgcgc
     2581 gcgtcctgtt ccgtcaacaa gaagacgtta ccgattacgg agcagcgcgt gtggtagcga
     2641 atccaatggc cgtcgttgat gccggcgagc actactccgg ggcgccgctc gcacgcctca
     2701 cgcagctgca tgagacccgg atacatgtcg gcgtagaacg gatcgccgcc ggccggccgc
     2761 ggggtgctcc acacggcagc cgtcggcgtc agcgccgcga ccagaacgac ggcggacgcg
     2821 atgcgctgcg ccgcgatcca tgcgggccag cgcttcgagc aggcgtcgag cgccaatgcg
     2881 agcgttgcga gcagcgggac gacgccgagc acgccgaacc gatattgcag ctgcatgagg
     2941 accaggaaca tggctgccgc aaccgcaaag aactgacggc ccggctcgtt ggcgcgcacc
     3001 gcccagtaga gattcgcaag caaagccagc ggcgcgagcc acatgagcca cgagaagagg
     3061 cgcgtcgact gcgcgggacc gaacaaagcg tacagcgcgt acgggctccg cgcttcggtg
     3121 atgtcgcgga gcacagcgag gtcgccgctc atgaactgcg cgccgagccc ggcactgcgc
     3181 agcaatggca ccgcgacggc ggcagcggcc agcgccagcc ccaaggcgct cgcgaggcgt
     3241 ggccgaacga tggccagcgc gatcgagaca acggccgtgg ccgcggccac gtagctgtga
     3301 aaccacgaca acgaatagaa ctcgaacacg ccgccgcgcc atggctcgga cggaatcgac
     3361 acgagcaacg tcgtcgtaac gagcgccgca gcgagcgcga gcacggcgcg gcgcgggggc
     3421 agcggctcgc ggcgtaacca ccgccacgcg agcgcgagca cgaccggcaa ctgcaggatg
     3481 aacagcccgt tctgaacggc gacggccaac ccgagcacga caccgagcag caccgctggc
     3541 accacgctcg cggtgctgcg gaagaacgct atccctgagc acgccgcgag cagcgcgcag
     3601 atgagctcgg cgaagtggtg atcgacgttg ccgaccgcga acgcgagaaa caccatcggc
     3661 aacacggcga aggccagcgt cgcgagcgcc gcagctgtca acgaaagatc gagctggcgc
     3721 cagagaagca cgagcagccc gacggcaagc acggcagcgg ctaccggcag atgcaataga
     3781 acggcggcag cgtccgactc ggcggcaaac ggcccgaacc atccgacgat cgtagccatg
     3841 gcggtatcga atccccacgg ccatggaacc caactgccgt cgggataatg aatcgacgcg
     3901 tcgaactgcc gaacgggctc gccggccatg acgacgtcga gaattcggcg cgcgtggtaa
     3961 aaggcgtcgg gactcgacgg cacgtacttt ccgtcgacgc gcgcgccggc gaccatcgtg
     4021 agtgcggcga cgacgagcac gagcgatcca aagatccaga ccaacgcgta acggaacgcc
     4081 ggtgtcactc cgagctcctc gtgcttcgtt cggccgggtg cccggcggag cgaagaatag
     4141 gcgacggcaa gactcgatgg cgtgcgctag gtcacttgcg acccgtcttc agtagcccga
     4201 cgtgatcggg tagcgccaat cgcggccgaa cgcgcggctg ctcacgcgca cgccgggcgg
     4261 cgcctgtcgg cgcttgtact cc
//
LOCUS       DASSNN010000436         4213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_676389, whole genome shotgun sequence.
ACCESSION   DASSNN010000436 DASSNN010000000
VERSION     DASSNN010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676389"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFL84_08170"
     CDS             complement(<1..202)
                     /locus_tag="VFL84_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608635.1"
                     /translation="MTRTTRCISAAIAMFSAATVFEGPVLLAQETCAEIPTAADRLAC
                     YDLVSPPKEVAPAAPDTASAPPA"
     gene            complement(205..1830)
                     /locus_tag="VFL84_08175"
     CDS             complement(205..1830)
                     /locus_tag="VFL84_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7608636.1"
                     /translation="MKLRVAAALALASLGLAATAFGQSLLEAAEAGEHDAALAALKAG
                     GDVGARGPDGTTPLIWAAYNGDAELARALLAAGADADAKNEFGSFALAEAAIGGYTDV
                     IAALLKGGADANQANNEGETPLMEVARTGNVEAATLLLDAGADVNAVELWGEQSAIMW
                     AAAQKQPAMVKLLASRGADVDAHGAVRNWERKVIREPRPKDMNQGGFTPLHYAAREGC
                     IECARELVAAGAKLNLQDPHGVTPLVLSLLNLHFDFAAYLVEAGADVNKWDLYGRSPL
                     YMAADTSTLPVAGNGSMVVLPSMDSLKAIDVARLLLDKGANVNAQLKRRPPYRNVPQD
                     RGGDSILSQGATPLLRAARAGDTPMVELLLEHGALVDLPSNQGVTPLMAAAGVEFGLR
                     VTRGRNRTDEGVLSTMRALLDAGADINARMLMEPRGESAAHLLVIEQRLSDYSYDYRG
                     RQVPSVRAKPHRTALHGAAMKGFTPIVKFLAENGADLYAKDANGATALDLAEGNYNEP
                     FLRQAAEPHAETAAVLKELMAAHPPTEQLSSAR"
     gene            complement(1827..3122)
                     /locus_tag="VFL84_08180"
     CDS             complement(1827..3122)
                     /locus_tag="VFL84_08180"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET7608637.1"
                     /translation="MAFITKKHISRRAVLQGAGAAIALPLLDAMLPAGVAWGQTAAGK
                     IPHRLAFVGFPHGAVMRHWKPEETGRDYTMTRILEPLAPYRQHMTIVTGLRNKPAESP
                     EPHEFIERTWLSCVSPKQAGVIGPDAGVTADQFAARHIGQETRMPSLELTTSQRGAQL
                     AWRTPTQSLPQEGNPRNVFYRLFGQGDNEAERAAIVKETGSILDRVMAQTTSLQNKLG
                     GQDRAAVGAYLDSVREIERRVQMASQADTSALDIPEAPIGVPNDIDAHFALMFDLMAL
                     AFQADLTRVITFSMDREASMRTYNNLSISEAFHPLSHHANDPGKQDRLAVIQNYHTKV
                     FARFVDRMSKTQEGDGSLLDHSMVLYGSNMSDSNRHNNDPLPSAILGHAKGRIKGGQH
                     LHYPQDSKHANLLLTLLERTEIPIESIGDSDGTFSEIAA"
     gene            complement(3133..>4213)
                     /locus_tag="VFL84_08185"
     CDS             complement(3133..>4213)
                     /locus_tag="VFL84_08185"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET7608638.1"
                     /translation="AVDDITLASKLSFFLWNTIPDDELRELAVRGELSKEPVLRAQVA
                     RMLKDPRAETLASNFVFHWLDLKRLEEVEPDTSIFPYASGRGDPREDYLTELELFAKS
                     LFDEDRSIVDFMTAKHTYLNERIALLYGINSVKGDRFQRVELEDSTRWGLLGKGAILM
                     AAAYPNRTSPVLRGSFILEYIVGAPPATPPPGVDAFPEAEIGTAKARTVREIMAKHRE
                     NPTCFACHGVMDPLGFALENFDAVGVWRDVDRYNGNVIDSAAELPDGTPVNGPDDLRE
                     ALLRNPEQFVQTFTERFLTYALGRTVDYRDMPAVRKIVREAAKKDYRFSELVWQVVAS
                     EPFRMRVAPEPPAPPASVVAQAAAQ"
BASE COUNT          623 a         1408 c         1457 g          725 t
ORIGIN      
        1 aggccggtgg ggcactggcc gtgtccggcg cggcgggggc tacttccttc gggggcgaca
       61 cgaggtcgta gcacgcgagg cggtcggccg ccgtcggaat ctcggcgcac gtctcttgag
      121 cgagcagaac cgggccttcg aaaacggtcg cggcggaaaa catcgcaatg gccgccgaga
      181 tgcagcgagt ggtccgcgtc acggctaacg cgcgctcgag agctgctcgg tcggcggatg
      241 ggccgccatc agctccttga ggacggcagc agtctcggcg tgcggctcgg ccgcctgccg
      301 caagaacggc tcgttgtaat tgccttccgc gagatcgaga gccgtcgcgc cgttcgcatc
      361 cttcgcatag agatcggcgc cgttctcggc gaggaacttc acgatcggcg tgaagccctt
      421 catcgccgcg ccatgcagcg ccgtgcggtg cggcttcgcg cgcacgctcg gcacctgccg
      481 gccgcgatag tcgtagctgt agtcgctcaa gcgctgctcg atgacgagca agtgcgcggc
      541 cgactcgccg cgcggctcca tgagcatccg cgcgttgatg tcggcgccgg cgtcgagcag
      601 cgcgcgcatc gtcgagagca cgccttcgtc ggtgcgattg cggccgcgcg tgacgcgcaa
      661 gccgaactcg acgcccgcgg ccgccatcag cggcgtgaca ccctggttgc tcggcaggtc
      721 gacgagcgcg ccgtgctcga gcaagagctc gaccatcggc gtgtcgccgg cgcgcgcggc
      781 gcgcaagagc ggcgtcgcgc cttgcgacag gatcgaatcg ccgccgcggt cttgcggcac
      841 gttgcggtac ggcggccggc gcttcagctg cgcgttgacg ttcgcgcctt tgtcgagcag
      901 caagcgcgcg acgtcgatcg ccttcagcga gtccatgctc ggcagcacga ccatcgagcc
      961 gttgccggcc acgggcagcg tgctggtgtc agcggccatg tacaaaggcg atcggccgta
     1021 caggtcccat ttgttgacgt cggcgccggc ttcgaccagg tacgccgcga aatcgaagtg
     1081 cagattcaac agcgatagga cgagcggcgt gacgccgtgc ggatcctgca aattcagttt
     1141 ggcgccggcg gcgacgagct cgcgcgcgca ctcgatgcag ccttcgcgag ccgcatagtg
     1201 cagcggtgtg aagccgccct ggttcatgtc cttcggccgc ggctcgcgga tgaccttgcg
     1261 ctcccagttg cgcaccgcgc cgtgcgcgtc gacgtcggcg ccgcgcgagg ccaggagctt
     1321 gaccatcgcc ggctgcttct gcgcggcggc ccacatgatc gcggactgct cgccccagag
     1381 ctcgaccgcg ttgacgtcgg cgccggcgtc gagcagcagc gtggccgctt cgacgttgcc
     1441 cgtgcgcgcg acctccatca gcggcgtctc gccctcgttg ttcgcctggt tcgcgtcggc
     1501 gccgcctttc agcagcgcgg cgatgacgtc cgtgtagccg ccgatcgcgg cctcggccag
     1561 cgcgaacgag ccgaactcat tcttcgcgtc ggcatcggcg ccggccgcga gcaacgctcg
     1621 cgcgagctcg gcatccccgt tgtacgcggc ccagatcagc ggcgtcgtgc cgtcgggccc
     1681 gcgcgcgccg acgtcgccgc cggctttcag cgcggcgaga gccgcatcgt gctcgccggc
     1741 ttcggccgct tcgagcagcg actggccgaa cgccgtcgcc gctaacccga gcgaagccaa
     1801 cgccaacgcg gcggcgacgc ggagcttcat gccgctatct cggagaacgt gccgtcactg
     1861 tcgccgatcg actcgatcgg aatctccgtg cgctcgagaa gcgtcagcag caggttcgcg
     1921 tgcttcgagt cctgcggata gtgcaagtgc tggccgcctt tgatgcggcc cttcgcgtga
     1981 ccgaggatcg cggacggcaa cggatcgttg ttgtggcgat tgctgtcgct catgttgctg
     2041 ccgtagagca ccatcgagtg atcgagcagc gagccgtcgc cttcctgagt cttcgacatg
     2101 cggtcgacga agcgggcgaa caccttggtg tgatagttct ggatcacggc gaggcggtcc
     2161 tgcttgcccg gatcgttcgc gtggtgcgag agcgggtgga acgcttccga gatgctcaag
     2221 ttgttgtacg tgcgcatgct cgcttcgcgg tccatcgaga acgtgatgac gcgcgtgaga
     2281 tccgcctgga acgcgagcgc catcaagtcg aacatcagcg cgaagtgcgc gtcgatgtcg
     2341 ttcggcacgc cgatcggcgc ttcggggatg tcgagcgcgc tcgtatcggc ttgcgaggcc
     2401 atctgcacgc gccgctcgat ctcgcgaacc gagtcgagat acgcgccgac cgcggcgcga
     2461 tcctggccgc cgagcttgtt ctgcaggctc gtcgtctgcg ccatgacgcg atccaagatg
     2521 ctgcccgtct ccttgacgat ggccgcgcgc tcggcttcgt tgtcgccctg gccgaacaag
     2581 cggtagaaca cgttgcgcgg gttgccttct tgcggcagcg actgcgtcgg cgtgcgccac
     2641 gcaagctgcg cgccgcgctg cgacgtcgtg agctcgagcg acggcatgcg cgtctcctga
     2701 ccgatgtggc gcgccgcgaa ctggtcggcc gtgacacccg catcggggcc gatcacgccg
     2761 gcttgcttcg gcgacacgca cgagagccag gtgcgctcga tgaactcgtg cggctcgggg
     2821 ctctcggcgg gtttgttgcg caagcccgtg acgatcgtca tgtgctggcg atacggagcg
     2881 agcggctcga ggattcgcgt catggtgtag tcgcggccgg tttcctcggg cttccagtgg
     2941 cgcatcacgg cgccgtgcgg aaagccgacg aaggcgagcc ggtgcggaat cttgcccgcg
     3001 gccgtctggc cccaggcgac gccggccggc agcatcgcat cgagcagcgg cagcgcgatc
     3061 gcggctcccg ctccttgcag caccgcacga cgcgagatgt gcttcttggt aatgaacgcc
     3121 atttgtcctc tcctactgtg ccgcagcttg tgcgacgacg gatgccggcg gcgcgggcgg
     3181 ttccggcgct acgcgcatgc ggaacggctc gctcgccacg acctgccaaa ccaactccga
     3241 gaaacgatag tcctttttcg ccgcctcgcg cacgatcttc cgcacggcgg gcatgtcccg
     3301 gtagtcgacc gtccggccga gcgcgtacgt caggaagcgc tccgtgaacg tctgcacgaa
     3361 ctgttccggg ttgcgcagca gcgcctcgcg caagtcgtcg ggcccgttga cgggcgtgcc
     3421 gtccggcagc tccgccgcgg aatcgatgac gttgccgttg tagcgatcga cgtcgcgcca
     3481 aacgccgacg gcatcgaagt tttcgagcgc gaagccgagc gggtccatga cgccgtggca
     3541 cgcgaagcac gtcggattct cgcggtgctt cgccatgatc tcgcgcaccg tgcgcgcctt
     3601 cgccgtgccg atctcggcct ccgggaacgc gtcgacgccc ggcggcggcg tcgcgggcgg
     3661 cgcgccgacg atgtactcga ggatgaacga tccgcgcagc accggcgacg tgcggttcgg
     3721 atacgcggcc gccatcagga ttgcgccttt gccgagcaag ccccagcgtg tggaatcttc
     3781 gagctcgacg cgctggaagc ggtcgccctt gacgctgttg atgccgtaca agagcgcgat
     3841 gcgctcattt agatacgtgt gcttcgccgt catgaagtcg acgatgctgc ggtcctcgtc
     3901 gaagaggctc ttcgcgaaca gctcgagctc ggtgaggtaa tcctcgcgcg gatcgccgcg
     3961 gcccgacgcg tacgggaaga tcgacgtgtc gggctcgacc tcctcgaggc gcttcaagtc
     4021 gagccagtgg aagacgaagt tgctggccag cgtctcggcg cgcgggtcct tgagcatgcg
     4081 cgcgacctgc gcgcgcagca cgggctcctt gctgagctcg cctctaacgg cgagctcgcg
     4141 cagctcgtcg tcggggatgg tattccagag gaagaacgac agcttcgacg ccagcgtgat
     4201 gtcgtcgacg gca
//
LOCUS       DASSNN010000437         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1307756, whole genome shotgun sequence.
ACCESSION   DASSNN010000437 DASSNN010000000
VERSION     DASSNN010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307756"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..774
                     /locus_tag="VFL84_08190"
     CDS             352..774
                     /locus_tag="VFL84_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7608639.1"
                     /translation="MDSNAPLIAIVDDEVSICRALLRLLRVTDYRAEAFNSPILFLES
                     LAKRVPDCVVLDLQMPMMTGVELQEHLARLADPPPVIIITAHDEPKTRERCLALGAVR
                     YLRKPIEGEMLIDSIEKAVRARRRALAKSHGDGVVDRP"
     gene            complement(817..1518)
                     /locus_tag="VFL84_08195"
     CDS             complement(817..1518)
                     /locus_tag="VFL84_08195"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HET7608640.1"
                     /translation="MTRRFKIAAFGVATLAAALVAGTGGAAPKQPRIETGPDAEVTFD
                     GLVRVQKSVVDAAWVKPNFDLTPYKKLMVVSQGVSFRKLEPVSEFQARKESQFPVKEE
                     NKERLSRELKTAFETELGKLERYEIVSQPGPDVLLLVGAVIDVVSNIPPDVDSARFGR
                     GGVYLTSIGDATLVLELRDSESGEILGRAADRRAAESPFAFEVNNVTAWSEVRRLASY
                     WATQLRRRLEEIEKV"
     gene            1703..2140
                     /locus_tag="VFL84_08200"
     CDS             1703..2140
                     /locus_tag="VFL84_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608641.1"
                     /translation="MQFRHPRDRTLRLLTGLAGVLYVGSVFADDLTGSDSLLCYGLSA
                     ARCATGEACESVEPWKLNIPDFVKLDLRAKRIQTTAASKDNRETPLQTVQRSNGTILV
                     QGLQGERAFSWLITETSGEGTLSVAGVGQGVTVFTVCTPVDKL"
BASE COUNT          367 a          745 c          771 g          413 t
ORIGIN      
        1 aggatcgcgg gcctcgcgga ccacatcgtg ctgcccgtat cgcacgtcgc gctgctgtgg
       61 tcggcgagcg ttgcggcgca ggtcgcgcac tttctcgagc agggccggtt ccggcacgag
      121 cccaagtagg tctagcgcgg cgccgacgac tggggggatc cgaccggctg ccgcgctagg
      181 gcgtgcatgt tgctcttttc gccgttgcta ctgtcgggcc tgggcggcgc gcgcgacatt
      241 cgaccttggt cccatgaagg ggggttgcgc cggccgagag tcgagtccga gcggtagaca
      301 tagccgaagg tcgcatgtcg ctttgccgcg gcaacgcgct caatgagcgc catggacagc
      361 aatgcgccgc tgatcgcgat cgtcgacgac gaggtttcga tttgccgcgc gttgctgcgc
      421 ctcctgcgcg tgacggatta ccgtgcggaa gcgttcaatt cgccgatctt gtttctcgag
      481 tcgctggcga agcgcgtgcc cgattgcgtc gtgctcgatt tgcaaatgcc gatgatgacg
      541 ggcgtcgagc tgcaggagca tctcgcgcgg ctcgcggatc cgccgcccgt gatcatcatc
      601 accgcgcatg acgagccgaa gacgcgcgag cgttgcctcg cgctcggcgc cgtgcgctat
      661 ttgcgcaaac cgatcgaggg cgaaatgctg atcgactcga tcgagaaggc cgtgcgcgcg
      721 cggcggcggg cgcttgcgaa atcccatgga gacggcgtcg tcgaccggcc gtaacgtcgc
      781 gtcgagctct cgacgcgcgg atccgcaggc gtcgcgtcag accttctcga tctcctcgag
      841 acgcctgcgc agctgcgtcg cccagtacga cgcgaggcgc cgcacctccg accacgccgt
      901 gacgttgttg acctcgaagg cgaacggcga ctcggcggcg cgccgatccg ccgcgcgccc
      961 gagaatctcg ccgctctccg agtcgcgcag ctcgagcacg agcgtcgcgt cgccgatcga
     1021 ggtcagatac acgccgccgc ggccgaagcg cgccgagtcg acgtcgggcg gaatgttcga
     1081 gacgacgtcg atgacggcgc cgacgagcaa caggacgtct ggcccgggct ggctgacgat
     1141 ctcgtagcgc tcgagcttgc cgagctccgt ttcgaacgcg gtcttgagct cgcgcgacag
     1201 gcgttccttg ttctcttcct tgaccgggaa ctgcgattcc ttgcgcgcct gaaactcgct
     1261 gacgggctcg agcttgcgga acgagacgcc ctggctcacg accatgagct tcttgtacgg
     1321 cgtcagatcg aaattcggct tgacccatgc tgcgtcgacg acgctcttct gcactcgaac
     1381 gagcccgtcg aacgtgacct cggcgtccgg cccggtctct atgcgcggtt gtttgggtgc
     1441 cgcgccgccc gtgccggcga cgagcgctgc ggccagcgtg gcgacaccga aggctgcgat
     1501 tttgaaacgg cgcgtcatga tcgaaccccc atacttctct ggctcgtgtt gaatgcccaa
     1561 ctctgcgcgc gatgttctgc cgcggcgcgc gacttgcaaa taggccgttg ggccatggtc
     1621 atccgcccta ggtcctatgg cccgcgcgcg aggtctgccg acaatgagcc gaggccgatc
     1681 ctgcggccgt cgggagatgt gcatgcagtt tcgccatccg cgcgacagga cactgaggct
     1741 tctgaccggc ctcgcgggcg tgctctacgt cggcagcgtg tttgccgacg acctgacggg
     1801 ctcggactcg ctgctctgct acggcttgtc ggcggcgcgc tgcgcgacgg gagaggcgtg
     1861 cgagtcggtg gagccgtgga agctcaatat cccggacttc gtcaagctcg acttgcgcgc
     1921 caagcgcatt cagacgaccg ccgcgagcaa agacaatcgc gaaacgccgc tgcaaacggt
     1981 ccagcgctcg aacggcacga ttttggttca aggcctgcaa ggcgagcgtg cgtttagttg
     2041 gctcattacc gagacgagcg gagagggcac gctcagcgtc gcgggcgtag ggcagggcgt
     2101 cacggtgttc acggtgtgca cgcccgtcga caagctgtag cggtcgatcg agatgcaatg
     2161 aggtgaccgt catgcgaaga agttcgactg ccgttctcat cgccgcgctc gctctgctcg
     2221 gcaacccagc cgccgcacaa agcgtgtcgt cgcaactggg cctcgtcgtt tacccgagca
     2281 acaagcagga cgccgc
//
LOCUS       DASSNN010000438         5223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_207330, whole genome shotgun sequence.
ACCESSION   DASSNN010000438 DASSNN010000000
VERSION     DASSNN010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207330"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFL84_08205"
     CDS             complement(<1..280)
                     /locus_tag="VFL84_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7608642.1"
                     /translation="MSAASVAVVDDDGSLRASLARLFRSAGYSVSVFETATELLSSLP
                     RSRLDCVVLDLQLPGMTGVELLRRFQVLVDPPPVIVITGSDDERLREQC"
     gene            534..1850
                     /locus_tag="VFL84_08210"
     CDS             534..1850
                     /locus_tag="VFL84_08210"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HET7608643.1"
                     /translation="MRAIDINDYAVGIGVATTENIYVDAGDSQTIYPYLTKLVPSALD
                     DGVTFGRDGAYGVRWLSSNGFEIGALGKLQTQGYEADDSPLFAGLADRAWTVEVGPTF
                     GWRGPVHVDWTAFVDLLRNHKGSNQLLRLSLPRAFPRGYLIPEVGFHRYTRQFVDYYY
                     GVPVEAAEPGRPAFEGEPANGLSLGIAWGVRLTPHWIFTGALDVERFGAEIADSPLVA
                     DDDQSRLTLQVTYDGAPFFAPDAAMQVPVNLDFGLAEIKGETADDSSNSLGWFEAALR
                     VARRHRVAVGGFDATYSPNAGAEILIRNLQLLYGYDVLDDRQKTVTVEAGLHVDKLSA
                     DDAALTLPGRTANPLPMLAVDAAARFESRLSLRARLQLLMLDGEGYSGRQMFAAFGLY
                     HRTFENVSFGVGYVFNRVALRTGNAELATLIEPLHQGPSVLISASF"
     gene            complement(1868..2173)
                     /locus_tag="VFL84_08215"
     CDS             complement(1868..2173)
                     /locus_tag="VFL84_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608644.1"
                     /translation="MKVREVLDTWPPDKWTADDGHGRAPTDDAGKLKLLWFSVPDAEG
                     WFSLTATDSSGASWSTYCRTSDDVWRALEHALHAALKQPLELVGNVEVEPVPRPRLE"
     gene            2285..3613
                     /locus_tag="VFL84_08220"
     CDS             2285..3613
                     /locus_tag="VFL84_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7608645.1"
                     /translation="MNRPAVVILLASILSAASAPGFAQNTRRQQAIASSDVLSQIPMF
                     SYREGKKSSLNFRGTPIAANAQGKADVQFEDGNTEISAKVDDLPAPGSLGPYTTYVLW
                     TLTPDGRAANQGVIGSHEGGKGEIKTGYGASQFALIVTAEPHFAVSAPSTMIALYNVA
                     DEVEGTESKVTSLAERFDYSALKPIAIDEDKSPAAIVQAKYAVAIAESVGARQYAPDT
                     YAAAAQKLGAAEASANAKKSSDRKAAAGFAREAVIASEDARRAAMVGAAAAKAEAERR
                     TVAAAAAEAERARSAAESAAKARLELLGRLDAALPTRVTERGLVSEIGGVQFATGTSD
                     LSGPAREALARFSGIVASYPGLELNVEGHTDNVGNPAANNELSLRRAIAVRDYLISQG
                     VAASSIDVAGLGSSRPAADNATAEGRARNRRVEIVVSGGPLDTSRAVAAR"
     gene            3642..4085
                     /locus_tag="VFL84_08225"
     CDS             3642..4085
                     /locus_tag="VFL84_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HET7608646.1"
                     /translation="MAESPRDLAGPRFVVDTTAGNRLAWFRTWTMIERTMSSWILTAI
                     TLIGFGFTIVLLFDELGRFTGLEPPVRPLAPFQFGLALIGVGVVALVVAGWQYRAILA
                     HLRHDELAGVAEGTQTPVQALVYAITIITIFIGVFAFLAVLVRAL"
     gene            4082..4633
                     /locus_tag="VFL84_08230"
     CDS             4082..4633
                     /locus_tag="VFL84_08230"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HET7608647.1"
                     /translation="MIRTSPLSANPHGLEPLLAQYWWVLFARGALAVVFGMTTLVWPG
                     LSFAVLLLFVASWFFADGAVALFQAFTSAQRWPHVLDSSLSIAAGAFVILYPAMAGLA
                     LTMTIAIWLIAKGVTQVLLALRFGGTHQGAWLLGVVGITTAGFGAFLAHNPADALGLM
                     TLISGFAVALGLSFVALGWWFER"
     gene            complement(4646..5008)
                     /locus_tag="VFL84_08235"
     CDS             complement(4646..5008)
                     /locus_tag="VFL84_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7608648.1"
                     /translation="MAVIDDDESVRTALVRLFRSVDFETFAFASARAFLSEFRDLTLD
                     CVVLDLQMPEMTGLDVQRYLKMVYPSLSLPVVMITAHDEPGSAESCLAAGVAAYMRKP
                     VESTALVAEVRRLIRTDH"
     gene            complement(5085..>5223)
                     /locus_tag="VFL84_08240"
     CDS             complement(5085..>5223)
                     /locus_tag="VFL84_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7608649.1"
                     /translation="QIAARLGTAEKTIKVHRARVMHKMSVRSVAELTRVAEIVGIRPE
                     P"
BASE COUNT          832 a         1759 c         1754 g          878 t
ORIGIN      
        1 tgcactgctc gcggaggcgc tcgtcgtcgc tgcccgtgat gacgatgacg ggcgggggat
       61 cgacgagcac ctggaaacgc cgcagcagct cgactccggt catgcccggc aactgcaagt
      121 cgagcaccac gcagtccagc ctgctccgcg gcagcgagct caagagctct gtcgccgtct
      181 cgaacaccga gaccgagtag ccggcggagc ggaacaggcg cgccaaagaa gcgcgcagcg
      241 acccgtcgtc gtcgacgacg gcgaccgacg cagcgctcat tccgttagcc ccctgtccgc
      301 gcgacaagct tcttgtgcga agcttggccg cacattcgac cgcttctgat aattttcgcg
      361 cgattgcacc gacagctgcc ccaatgacac cgccggcatc tcgattgagg ctcatcgata
      421 ctggcaagtc aagccgcgtt cgtcgtgcgt gcaggcgcgc gcagctacgc gcaagcgcgg
      481 ccgcatgcct gctcgggctc gccgcgccgt gcagcgcagg ggtgctcgac agcttgcgag
      541 ccatcgacat caacgactat gcggtcggca tcggcgtcgc gacgaccgag aacatctatg
      601 tcgacgcggg cgactcgcag acgatctatc cgtatctgac gaagctcgtg ccgtcggccc
      661 tcgatgacgg cgtgacgttc gggcgcgacg gcgcctacgg cgtccgctgg ctctcgagca
      721 acggcttcga gatcggcgcg ctcggcaagc ttcagacgca gggctacgaa gccgacgact
      781 cgccgctgtt tgccggactg gccgaccgcg cctggaccgt cgaggtcggt ccgacgttcg
      841 gctggcgcgg ccccgtgcac gtcgactgga ccgcgttcgt cgatctgctc cgcaatcaca
      901 aaggctcgaa tcagctgctc aggctgtcgc tgccgcgcgc gttcccgcgc ggctacctga
      961 taccggaagt ggggttccat cgctacacgc gccaattcgt cgactactac tacggcgttc
     1021 ccgtcgaggc cgccgagccg ggcagaccgg ccttcgaagg cgagcccgcc aacggcctgt
     1081 cgttgggcat tgcttggggc gtgcgcctga cgccgcattg gatcttcacg ggcgccttgg
     1141 acgtcgagcg gttcggcgcc gagatcgcgg acagccctct cgtcgccgac gacgaccaga
     1201 gcaggctcac gttgcaggtg acctacgacg gcgctccgtt ctttgcgccg gacgctgcaa
     1261 tgcaggttcc cgtgaatctc gacttcggcc tggcggaaat caaaggcgag acggcggacg
     1321 actcgagcaa ttctctcggt tggttcgagg cggcgctgcg cgtcgcgcga cggcatcgcg
     1381 tcgcggtcgg cggcttcgac gcaacgtatt cgccaaacgc cggcgcggag attctgattc
     1441 gcaatctcca gctgctctac ggctacgacg tgctcgacga tcggcaaaag accgtgacgg
     1501 tcgaagcggg cttgcacgtc gacaagctct ccgcggacga cgccgcgctt acgttgcccg
     1561 gccgtaccgc gaatccgctg ccgatgctgg ccgtcgacgc cgccgcgcgc ttcgagagcc
     1621 ggttgtcgct cagagcgcgg ctgcagctcc tgatgctcga cggcgaaggc tactcgggcc
     1681 ggcagatgtt cgcggcgttc ggcctttacc atcggacgtt cgagaacgtg agcttcggcg
     1741 tcggctacgt cttcaatcgc gtggcgctgc gcaccggcaa cgcggagctc gcgacactga
     1801 tcgagccgct gcatcagggg ccgagcgtgc tgatctcggc gtcgttctag cgcagcgaaa
     1861 ggggcggcta ctcgagccgc gggcgcggca cgggttcgac ctcgacgttg ccgacgagct
     1921 cgagcggctg cttcaaagcc gcgtgcagcg cgtgctcgag ggcacgccag acgtcgtcgc
     1981 tcgtgcggca gtacgtcgac cagctcgcac cgctcgaatc ggtggccgtc aagctgaacc
     2041 agccttccgc gtcgggaacg ctgaaccaga gcagcttgag cttcccggcg tcgtccgtcg
     2101 gagcacggcc gtgaccgtcg tcggccgtcc acttgtcggg cggccaggtg tcgagaacct
     2161 cacgtacctt catgccgacg acaggatctt cgtccgagcg cgcgcgggct gtgcgaccaa
     2221 ggtccgatgg cgacgaccca tcggcaggtc gagagtcggg cctattcgac agcggagggg
     2281 cgccatgaac cgaccagccg tcgttatcct tttggcttcc attctcagcg ccgcatcggc
     2341 gcccgggttc gctcagaaca ctcgtcggca gcaagcgatc gcgagctccg acgtgctgag
     2401 ccagatcccg atgttctcgt accgcgaagg caaaaaatcg agcctgaact tccgcggcac
     2461 gccgatcgcc gccaacgcgc agggcaaggc cgatgtccag ttcgaggacg gcaacaccga
     2521 gatttccgcg aaggtcgacg atcttcccgc gccgggatcg ctcggaccgt acacgaccta
     2581 cgtgctctgg acgctgacgc cggacggccg cgcggcgaat caaggcgtca tcggcagcca
     2641 cgagggcggc aaaggcgaga tcaagaccgg atacggcgcc tcgcagttcg ctttgatcgt
     2701 gacggccgag ccgcacttcg ccgtcagcgc tccgagcacg atgatcgcgc tctacaacgt
     2761 agccgacgag gtcgaaggca ccgagtcgaa ggtcacgtcg ctggcggaac gcttcgacta
     2821 ctcggcgctg aagcccattg cgatcgacga ggacaagagc ccggcggcga tcgtgcaagc
     2881 gaaatacgcc gtcgcgatcg cggagtccgt cggcgcgcgg cagtacgcgc ccgacactta
     2941 cgcggccgcg gcgcaaaagc tcggcgccgc ggaagcttct gccaacgcga aaaagagctc
     3001 cgatcgcaag gcggcagccg ggttcgcgcg cgaggccgtg atcgcgagcg aggacgcgcg
     3061 gcgggccgca atggtcggcg ccgccgcggc gaaggccgaa gccgagcgcc gaaccgttgc
     3121 ggccgcggcc gccgaggccg agcgcgcgcg ctccgcggcc gagagcgcgg cgaaggcgcg
     3181 tctcgagctg ctcggccgtc tcgacgcggc gctgccgacg cgcgtcacgg agcgcggcct
     3241 cgtgtccgag atcggcggtg tgcaattcgc gaccggcacg tcggacttga gcggcccggc
     3301 gcgtgaagcg ctggcgcggt tctcgggaat agtcgcgtcg tatccgggcc tcgagctcaa
     3361 cgtcgaaggg cacaccgaca acgtcggcaa cccggcggcc aacaacgagc tctcgctgcg
     3421 ccgcgcgatc gcggtccgcg actacttgat cagccaggga gtcgcggcgt cgagcatcga
     3481 cgtcgcggga ttgggctcgt cgcgccctgc ggccgacaac gcgacggccg aaggacgcgc
     3541 gcggaatcgc cgcgtcgaga tcgtcgtgtc gggcggcccg ctcgacacga gccgcgcggt
     3601 cgcggcgcgc tgacccctcg agcggcacgt gtgtaacggc aatggctgaa tcgcctcgcg
     3661 accttgctgg acccagattc gtcgtcgaca cgacggcggg caatcgcctc gcttggtttc
     3721 ggacctggac gatgatcgag cgcacgatgt cgtcttggat cctcacggcg atcacgctga
     3781 tcggcttcgg cttcacgatc gtgctgttgt tcgacgagct cggtcgcttc acggggctgg
     3841 agccgccggt ccgcccgctc gctccgtttc agttcggtct cgcgctgatc ggcgtgggcg
     3901 tcgtcgcgct cgtcgtcgcg gggtggcagt accgcgcaat cctcgcccat ctgcggcacg
     3961 acgagctcgc cggcgtggca gaaggcacgc aaacaccggt gcaggcgctc gtctacgcga
     4021 tcacgatcat cacgatcttc atcggcgtat tcgcgttcct cgccgtcctg gtgcgcgcgc
     4081 tgtgattcga acgagcccgc tttcggccaa cccgcacgga ttggagccgt tgctcgcgca
     4141 gtactggtgg gtgctgttcg cgcgcggcgc gcttgcggtc gtcttcggca tgacgacgct
     4201 cgtgtggccg ggcctttcgt tcgccgttct gctgctgttc gtcgcgagct ggttcttcgc
     4261 cgacggcgca gtcgccttgt ttcaggcgtt cacttcggcg cagcgctggc ctcatgtgct
     4321 cgacagctcg ctcagcatcg cggccggcgc gttcgtgatc ttgtatcccg cgatggccgg
     4381 cttggctctc acgatgacga tcgcgatctg gctgatcgca aaaggcgtga cgcaagtgct
     4441 gctggctttg cgcttcggcg gcacgcatca gggcgcatgg ctgctcggcg tcgtcggcat
     4501 cacgaccgcg ggattcggcg ccttcctcgc gcacaacccg gccgacgctc tcggcttgat
     4561 gacgctgatc tctggattcg ccgtcgcgct cgggctgtcg ttcgtcgctc tcggctggtg
     4621 gttcgagcgc tgagctcaag cgcggtcagt gatccgtccg gatcaaccgg cggacctcgg
     4681 cgacgagcgc cgtgctctcg acgggcttgc gcatgtacgc cgcgacgccg gcggccaggc
     4741 aactttcggc gctgccgggc tcgtcgtgcg ccgtgatcat gaccacgggc agcgacaagc
     4801 tcgggtagac catcttcagg tagcgctgga cgtcgaggcc cgtcatctcg ggcatctgca
     4861 agtcgagcac gacgcagtcg agcgtcaagt cgcggaattc actcaagaaa gcacgagccg
     4921 atgcgaacgc gaacgtctcg aagtcgacgg accgaaagag gcgtacgagc gcggttcgaa
     4981 ccgactcgtc gtcatcgata acggccacgc ggtgtcgacg catgggctct atttacaccc
     5041 ccgcgccggc cggacgctat gggcgcttgt gcctacgctt gagctcaggg ctcgggccga
     5101 atgccgacga tctcggccac gcgcgtcagc tcggcgacgg agcgcaccga catcttgtgc
     5161 atgacgcgcg cacggtgaac cttgatggtc ttctcggcgg taccgagcct cgcggcgatc
     5221 tgc
//
LOCUS       DASSNN010000439         2911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1090105, whole genome shotgun sequence.
ACCESSION   DASSNN010000439 DASSNN010000000
VERSION     DASSNN010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090105"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1028
                     /gene="gshB"
                     /locus_tag="VFL84_08245"
     CDS             60..1028
                     /gene="gshB"
                     /locus_tag="VFL84_08245"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HET7608650.1"
                     /translation="MTQPRRLLGVVMDPIDSIKPKKDSTLAMLLAAQRRGWDIVYFRQ
                     HDLGVRDGAPKGHGSRLKVRDDSERWFEIGVPWSGGLETLDALLMRKDPPFDMEYIYT
                     TYILELAENRGLLVVNKPASLRDINEKAYTSWFPQCTPPSLVTRSRTALLEFLKEQGK
                     VVLKPFDGMGGRSIFVVAVGDPNTNVIIETLTDDGERFTLAQRFIPEIAQGDKRVLLI
                     DGVPIDYALARIPAPGESRGNLVMGARGEGRPLTERDRWLCAQVGPTLAAKGVLFAGL
                     DVIGDYLTEINVTSPTGIRELDRQFNIDIAAKLIDVIDRKLAAKAA"
     gene            1049..1903
                     /locus_tag="VFL84_08250"
     CDS             1049..1903
                     /locus_tag="VFL84_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET7608651.1"
                     /translation="MSDERLGSTLFLAALVHGVVILGVTFTAATTSDDTAPSLNVTLV
                     LDTGREELAADTAEFISNRNQQGAGVAARGLRPTSSLSADQPASQLGDPLGADASDGT
                     PREARPSAEQLASQGNVGDPVPALPQPIEAPAPTLQRAAALIEQAPQTTALEVGLRAE
                     LPNGNDDARTLIATPSTRESGLAEYLDGWRRRVERIGTANYPNELLGNIHAGRPTLEV
                     VIAADGRLDDIVVRRSSGDKALDQAALKILRLAAPFPPLPPSIREDYDVLRFAYEWDF
                     AAGGESGR"
     gene            1958..2512
                     /locus_tag="VFL84_08255"
     CDS             1958..2512
                     /locus_tag="VFL84_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET7608652.1"
                     /translation="MQSLQDHFLIAMPAMADPNFNETVTYICKHDAEGAFGLVITRPS
                     DLSLGEMLGQLAIELKDRRLAERPVMHGGPVEPERGFVLHRSDTPFEATLAVGPEIKL
                     TSSPDILAAIGNGYGPDPAFVALGYAGWGRGQLEAELGSNTWLTVPANPGIIFDTPIE
                     QRWTSALGLPGVDIRRITQYAGHA"
     gene            2530..>2911
                     /gene="ruvX"
                     /locus_tag="VFL84_08260"
     CDS             2530..>2911
                     /gene="ruvX"
                     /locus_tag="VFL84_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET7608653.1"
                     /translation="MARLTERDRDRHLVVAFDFGLRRIGVATANRATRTASPLTTVRV
                     EREPPWRELDRIIDDWRPAQLVVGVPEGDGAAAVARRARGFAAELAERYGLPVATVDE
                     TLTSAAAESELAEKRRSGYLRRRVG"
BASE COUNT          497 a         1003 c          964 g          447 t
ORIGIN      
        1 gtagctttgc gctttttcgc gaaactgcga acccgcgcgc aaaagcgagg aatgttttca
       61 tgacccagcc ccggcgattg ctcggcgtcg tgatggaccc gatcgactcc atcaagccca
      121 agaaggactc gaccctcgcc atgctgctgg ccgcccagcg ccgcggctgg gacatcgtct
      181 atttccggca gcacgacctc ggggtccgcg acggcgcgcc gaaggggcat ggctcgcggc
      241 tcaaggtccg cgacgacagc gagcggtggt tcgagatcgg cgtgccctgg agcggcgggc
      301 tcgagacgct cgacgccttg ctgatgcgca aggacccgcc gttcgacatg gagtacatct
      361 acacgaccta cattctcgag ctcgccgaaa accgcggcct gctcgtcgtc aacaagcccg
      421 cgagcctgcg cgacatcaac gagaaggcct atacgtcctg gttcccgcag tgcacgccgc
      481 cgagcttggt cacgcgctcg cgtaccgccc tgctcgagtt cctgaaggag cagggcaagg
      541 tcgtcctgaa gccgttcgac ggaatgggcg gccgctcgat cttcgtcgta gccgtcggcg
      601 atcccaacac gaacgtgatc atcgagacgc tgaccgatga tggcgagcgg ttcacgctcg
      661 cgcagcgctt catcccggag atcgctcagg gcgacaaacg cgtcctgttg atcgacggcg
      721 tcccgatcga ctacgcgctc gcgcgaatac cggcgcccgg cgaaagccgc ggcaacctcg
      781 tcatgggcgc gcgcggcgag gggcgcccgt tgacggagcg cgatcgctgg ctatgcgcgc
      841 aagtgggacc gacgctggcg gcgaaaggcg tcctgttcgc aggcctcgac gtcatcggcg
      901 attatctgac cgagatcaac gtgacttccc ctacgggaat ccgtgaactc gatcggcagt
      961 tcaacatcga catcgctgct aagctcatcg acgtcatcga tcgcaagctg gcggccaagg
     1021 ccgcctgacg ctatcgtaac ttccgctcat gagcgatgaa cgactcgggt cgacgctttt
     1081 cctcgccgcg ctcgtccacg gcgtcgtgat tctcggcgtg acgttcactg ccgcgacgac
     1141 cagcgacgac accgctccct cgctgaacgt cacgctcgtg ctcgacacgg gacgcgaaga
     1201 gctcgccgcc gatacggcgg agttcatctc gaaccgcaac cagcaagggg ccggcgtggc
     1261 ggcgcgcggc ttgcggccga cgagctcgtt gtcggccgac caaccggcga gccagctcgg
     1321 agatccgctc ggcgccgacg catccgacgg cactccgcgc gaagcgaggc cgagcgcgga
     1381 gcagctcgcg agccagggca acgtcggcga ccccgtaccc gcgttgccgc agccgatcga
     1441 ggcgccagcg ccgacgctgc agcgtgcggc cgcgttgatc gagcaagcgc cgcagacgac
     1501 ggcgctggag gtcgggctca gagccgagct gccgaacggc aacgacgacg cacgaacact
     1561 aatcgcaacg ccgagcacgc gtgagtcggg tctcgccgaa tacttggacg gctggcggcg
     1621 gcgcgtggag cgaatcggca ccgcgaatta tccgaacgag cttctcggca acattcacgc
     1681 gggacgcccg acgctcgagg tcgtgatcgc cgcggacggc cggctcgacg acatcgtcgt
     1741 gcggcgctcg tcgggcgaca aggcgctcga tcaagccgcg ctgaagatcc tgcggctcgc
     1801 ggctccgttt cctccgctgc cgccaagcat tcgcgaggac tacgacgttc tgcggttcgc
     1861 gtacgaatgg gacttcgccg ccgggggcga gagcgggcgt tgagcgcgcc gtcgatgcta
     1921 cgataacggc atcgacttga acgggagctc gccgccgatg caatcgctgc aagatcattt
     1981 cttgatcgcg atgcccgcga tggcggatcc gaatttcaac gagaccgtca cttatatctg
     2041 caagcacgac gccgagggcg cgttcggcct cgtcatcacg cggccgagcg acttgtcgct
     2101 cggcgagatg ctgggccagc tggccatcga gctcaaagat cgccgcctgg ccgagcggcc
     2161 cgtcatgcac ggcggccccg tcgagccaga gcgcggcttc gtgctgcacc ggtccgatac
     2221 gccgttcgag gccacgctcg ccgtcggtcc cgagatcaag ctgacctcat cgcccgacat
     2281 actggcggcg atcggcaatg ggtacgggcc ggacccggcg ttcgtcgcgc tcgggtacgc
     2341 gggctggggg cgcgggcagc tcgaagccga gctcggcagc aacacgtggc tcacggtccc
     2401 tgcgaacccg ggcatcatct tcgacacgcc gatcgagcag cgctggacgt ccgctctcgg
     2461 gttacccggc gtcgacattc gccgcattac gcaatatgcg ggtcacgcct gaagcgtaag
     2521 gatggcgccg tggcgcggct cactgaacgc gaccgcgatc gccatctcgt cgtagcgttc
     2581 gacttcgggc tccgccgcat cggcgtcgct accgcgaacc gcgcaacgcg aaccgcgtcg
     2641 ccgctcacga ccgtacgcgt cgagcgcgag ccgccgtggc gcgagctcga ccgcatcatc
     2701 gacgattggc gccctgcgca gctcgtagtc ggcgtgccgg aaggagacgg cgccgcagcg
     2761 gtcgcgcgac gcgcgcgcgg tttcgcggcg gagctggccg agcgctacgg cttgcccgtc
     2821 gcgaccgtcg acgagacgct gacctcggcc gccgccgagt ccgagctcgc ggaaaagcgc
     2881 cgctccggct atcttcgccg ccgcgtaggc a
//
LOCUS       DASSNN010000440         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1308085, whole genome shotgun sequence.
ACCESSION   DASSNN010000440 DASSNN010000000
VERSION     DASSNN010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308085"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VFL84_08265"
     CDS             <1..1540
                     /locus_tag="VFL84_08265"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose/sorbosone family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HET7608654.1"
                     /translation="MRDNVLLHGGFQVEFACQIVHRMPGMRRPAVTAFLLLLPALAAA
                     QVASGPNANPRAVGSSPPERFTTRVVATGFEDPWEVTYGPDGWLWVTERVGKRVVRVN
                     PVDGSRKVAVTIGEVHQELAQDGLLGLALHPQLLRGSDHVYVMYTYDADSGAATERRA
                     KIRRYTYDTASQTLREPVDVLTNLPHGPDHGASRIVFGPDGKLYASRGDHGSNFLAYY
                     CEPIRAQELPTAADVAARNWRGYEGKILRVNVDGGIPADNPVLNGVRSHVFTYGHRNP
                     QGLAFGPDGRLYESEHGQDTDDEVNRIEAGRNYGWPLIAGYKDDQYYTYANWSASAPT
                     PCSSLDYGRTIPSTVPQTKESEVSLPDFTPPLKTFFTVPSTFDRRALGNATAALAGLD
                     VYTSAAIPGWNPSILVAGMMSGTIFRFPLSGGTDPPQTYFKAQDRYRDLAISPDGKRI
                     YAVTDAHGRVLTAAGEPTAELAHPGALLEFTYAVQSSASSEADDWRELRLDGDDDPAS
                     RVKR"
     gene            complement(1549..>2788)
                     /locus_tag="VFL84_08270"
     CDS             complement(1549..>2788)
                     /locus_tag="VFL84_08270"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET7608655.1"
                     /translation="YQKAGNYRGQHTDIEDVFLKTGYLTSGAVTLEEVRGMADVAIQS
                     PGVCQGMGTANSMHVVCEALGMTLPGSAPVAAGSAKMHENLRASGARIVEMVWADLKP
                     RDIITAGAVRNAVQMVLAVSGSINCVKHLQAIATEADADVDVYQAFADYADRVPLLAA
                     VKPTGTGHIEDFEAAGGARAVLKRLERQLDLDARSVSGQTLREILAATTVADERAIAT
                     LDEPLSKRPAIVIVRGSLLPGGGIVRLGGTGERMLSFKGTANIFHSRDEALAGIKAGK
                     VKPGDVVVLRGLGVIGGPGMAMTSAVIFALDGAGLLDRVAAITEGQLSGLVNNGLVVG
                     EASPEAAAGGPLAFVENGDVISIDVKRKVVDLEVPDSVLAERRKTARTYGAQDQRGWL
                     GVYQRTVAPVHEGAVLSRKP"
BASE COUNT          441 a          973 c          934 g          440 t
ORIGIN      
        1 gatgcgcgac aatgttctgc tgcatggcgg attccaagta gagttcgcgt gccagatagt
       61 acataggatg cccgggatgc gtagaccagc cgtgactgcg ttcctgctgt tgcttccggc
      121 cttggccgca gcccaagtcg catccggccc gaacgcgaac ccgcgcgccg tggggtctag
      181 cccgccggag cggttcacga cacgcgtcgt cgcgaccggg ttcgaggatc cgtgggaagt
      241 cacgtacggg cccgacggtt ggctctgggt caccgagcgc gtcggcaagc gcgtcgtgcg
      301 cgtgaacccg gtcgatggaa gccgcaaagt tgcggtcacg atcggcgagg ttcaccagga
      361 gctcgcgcag gacgggctgc tcgggctcgc gctgcacccg cagctcttgc gcggcagcga
      421 ccatgtctac gtcatgtaca cgtacgacgc ggactcgggc gcggccacgg agcggcgcgc
      481 gaagattcgc cgctatacgt acgacaccgc ttcgcagacg cttcgcgagc ccgtcgacgt
      541 gctgacgaat ctgccgcatg gccccgatca cggcgcgagc cgcatcgtgt tcggtcccga
      601 cggcaagctc tatgcgagcc gcggcgacca cggctctaat ttcctggcgt actactgcga
      661 gccgattcgc gcgcaggagc tgccgaccgc ggccgacgtc gcggcgcgca actggcgcgg
      721 gtacgagggc aagatcctgc gcgtgaacgt ggacggcggg atcccggccg acaatccggt
      781 gttgaacggc gtgcgcagcc atgtgttcac gtacggccac cgcaacccgc aaggtctcgc
      841 gttcggcccc gacgggcggc tctacgaatc cgagcacggc caggacacgg acgacgaggt
      901 caaccgcatc gaggccggac gcaactacgg ctggccgctg attgccggct acaaggacga
      961 ccagtactac acgtacgcga actggtccgc gtcggcgccg acgccgtgct cgagcctgga
     1021 ctacggccgc accatcccga gcacggtgcc gcaaacgaag gagtccgagg tgagcctgcc
     1081 ggacttcacg ccgccgctga agacgttctt cacggtgccg agcactttcg acaggcgcgc
     1141 gctcggcaac gcgacggcgg cgctcgcggg tctcgacgtc tacacgtcgg cggcgatccc
     1201 gggctggaat ccgtcgatcc ttgtcgccgg catgatgagc ggcacgatct tccgcttccc
     1261 gctgtcgggc ggcaccgacc cgccgcagac gtacttcaaa gcacaagatc gctaccgcga
     1321 tctcgcgatc tcacccgatg gcaagcgcat ctacgccgtc acggacgcgc acggccgcgt
     1381 gctgacggcg gccggcgagc cgacggccga gctcgcccat cccggcgcgc tgctcgagtt
     1441 cacgtacgcc gtgcagagct cggcgtcgtc tgaagcggac gattggcgcg agctgcgcct
     1501 cgacggcgac gacgatcccg cgagccgagt aaagcgctga ggccgcgctc agggtttgcg
     1561 cgagagaacc gccccctcgt gcacgggcgc gaccgtgcgc tgatagacgc cgagccagcc
     1621 gcgctgatcc tgcgcgccat acgtgcgcgc cgtcttgcgc cgctccgcga gcacgctgtc
     1681 gggcacctcg aggtcgacga ccttgcgctt cacgtcgatc gagatgacgt cgccgttctc
     1741 gacgaacgcg agcggcccgc cggccgcggc ttcgggcgag gcttcgccga cgacgaggcc
     1801 gttgttcacg agccccgaga gctggccttc cgtgatcgcg gcgacacggt cgagcaagcc
     1861 cgcaccgtcg agcgcgaaga tgacggccga cgtcatcgcc atgcccgggc cgccgatgac
     1921 gccaaggccg cgcagcacga cgacgtcgcc gggtttcact ttcccggctt tgatgccggc
     1981 cagcgcttcg tcgcgcgagt gaaagatatt cgccgtgcct ttgaacgaga gcatgcgctc
     2041 gcccgtgccg ccgaggcgca cgatgccgcc gcccggcagc aggctgccgc gcacgatgac
     2101 gatcgcgggc cgcttcgaca acggctcgtc cagcgtggcg atcgcgcgct cgtccgcgac
     2161 cgtcgttgcc gcgaggatct cccgcagcgt ttgcccggac accgaacgcg cgtcgagatc
     2221 gagctgtcgc tcgagccgtt tcagcacggc acgagcgccg ccggcggcct cgaagtcctc
     2281 gatgtggccc gtgccggtgg gtttcaccgc tgcgagcaat ggcacgcggt ccgcgtaatc
     2341 ggcgaaggct tgatacacgt cgacgtccgc gtccgcttcg gtcgcgatcg cctgcagatg
     2401 cttgacgcag ttgatcgagc cgctgacggc caagaccatc tgcacggcgt tgcgcacggc
     2461 gccggccgtg atgatgtcgc gcggcttcaa gtctgcccaa accatctcga cgatgcgcgc
     2521 tccggacgcg cgcaggttct cgtgcatctt cgcgctgccg gccgcgaccg gcgcgctgcc
     2581 gggcagcgtc atgccgagcg cttcgcagac gacgtgcatc gagtttgccg tgcccatgcc
     2641 ttggcaaacg ccggggctct ggatcgcgac gtcggccatg cccctgacct cttcgagcgt
     2701 cacggccccc gaggtcaagt atccggtctt caggaacacg tcctcgatgt ccgtgtgctg
     2761 gccgcgatag ttgccggctt tctggtag
//
LOCUS       DASSNN010000441         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1209371, whole genome shotgun sequence.
ACCESSION   DASSNN010000441 DASSNN010000000
VERSION     DASSNN010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209371"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1954)
                     /gene="parC"
                     /locus_tag="VFL84_08275"
     CDS             complement(<1..1954)
                     /gene="parC"
                     /locus_tag="VFL84_08275"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET7608656.1"
                     /translation="MRDQELNFDGIERVPLATFTEKAYLDYSMYVILDRALPHLGDGL
                     KPVQRRIIYAMSDLGLSATSKPKKAARTVGDVIGKFHPHGDTACYEAMVHMAQPFSFR
                     YPFVDGQGNWGSQDDPKSFAAMRYTEARLTRYAQVLLAELEQGTVDWQPNFDGTLEEP
                     KLLPAQLPNLLLNGTSGIAVGMSTDVPPHNLKEVANALIKLLDDPDATLGELMKSIKG
                     PDFPTGGELVSSRADLVSIYKTGTGTLRLRARYEIEDGEVVITELPFQTSGAKVLEQI
                     AAQMTAKKLPMVEDLRDESDHENPTRLVIAAKSKVDLDALMNHLFATTELERTVRVNV
                     NVIGLDGRPAVFGLKALLEEWLKFRTATVKRRLEHRLERVAQRLHILDGLLVAYLNID
                     EVIKIIRREDEPKPVLMKRFKISDAQAEAILELKLRHLAKLEEMQIKGEQKQLSEERA
                     DLEATLKSKAKLTKLIKAELEELAEKHGDKRRTVVVEREAAQAIDESELVTSEPVTVV
                     LSERGFARAAKGHDIDVTGLSYKAGDQFLAAARGKSNQLAVFIDSTGRAYSVGAHTLP
                     SARGQGEPLAGFFNPPDGASFRAVLIGAPEDKWVVASSAGYGFVVQLGELYANKKAGK
                     TALRVPEGGTVVPASPVGAAGTRLAAA"
BASE COUNT          292 a          690 c          686 g          388 t
ORIGIN      
        1 acgccgctgc gagccgcgtg cctgcggcgc cgaccggcga cgccggcacg acggtgccgc
       61 cttcgggcac gcgcagcgcc gtcttgcccg ctttcttgtt cgcgtagagc tctccgagct
      121 gcacgacgaa tccgtagccc gccgacgacg cgacgaccca cttgtcctcg ggcgcgccga
      181 tcagcacggc gcggaagctc gcgccgtcgg gcgggttgaa gaatcccgcg agcggctccc
      241 cctgcccgcg cgccgacggt agcgtgtgcg cgccgacgct gtacgcgcgg cccgtgctgt
      301 cgatgaacac ggcgagctgg ttgctcttgc cgcgcgcggc ggcgaggaac tgatcgccgg
      361 ccttgtacga caagcccgtg acgtcgatgt cgtggccctt cgccgcgcgc gcgaagccgc
      421 gctcggagag cacgaccgtg acgggctcgc tcgtgacgag ctccgactcg tcgatcgcct
      481 gcgcggcttc gcgctcgacg acgacggtgc ggcgcttgtc gccgtgcttc tcggcgagct
      541 cttcgagctc cgccttgatg agcttcgtga gctttgcctt ggacttcagc gtcgcctcga
      601 gatcggcgcg ctcctccgag agctgcttct gctcgccctt gatctgcatc tcctcgagct
      661 tcgcgaggtg gcgcagcttg agctcgagga tcgcctcggc ttgcgcgtcg gagatcttga
      721 agcgcttcat gaggaccggc ttcggctcgt cctcgcggcg gatgatcttg atgacttcgt
      781 cgatgttgag atacgcgacg agcaagccgt ccaagatgtg cagccgctgc gcgacgcgct
      841 cgaggcgatg ctcgagccgg cgcttgaccg tcgcggtgcg gaacttcagc cactcctcga
      901 gcagcgcttt gagcccgaaa accgcgggcc ggccgtcgag gccgatcacg ttgacgttga
      961 cgcgaaccgt gcgctcgagc tcggtcgtcg cgaacaagtg gttcatcagc gcgtcgaggt
     1021 cgaccttcga cttcgcggcg atgacgaggc gtgtcgggtt ctcgtgatcg gactcgtcgc
     1081 gcaggtcctc gaccatcggc agcttcttcg ccgtcatctg cgccgcgatc tgctcgagca
     1141 ctttcgcgcc cgacgtctgg aacggcagct ccgtgatgac gacctcgccg tcctcgatct
     1201 catagcgcgc gcgcaggcgc agcgtgccgg tgcccgtctt gtagatcgag acgagatccg
     1261 cgcggctcga cactagctcg ccgcccgtcg ggaagtcggg ccccttgatg ctcttcatga
     1321 gctcgccgag cgtcgcgtcg ggatcgtcga gcagcttgat cagcgcgttc gcgacttcct
     1381 tgagattgtg cggcggaacg tccgtcgaca tgccgacggc gatcccgctc gtgccgttca
     1441 ggagcaggtt cggcagctgc gccggcaaga gcttcggctc ttcgagcgtg ccgtcgaagt
     1501 tcggttgcca gtcgacggtg ccctgctcga gctccgctag cagcacctgc gcgtagcgcg
     1561 tgagccgcgc ctcggtgtag cgcatggccg cgaacgactt cgggtcgtcc tgcgagcccc
     1621 agttgccttg gccgtcgacg aacgggtagc ggaagctgaa cggctgcgcc atgtggacca
     1681 tggcttcgta gcacgccgtg tcgccgtgcg gatggaactt gccgatgacg tcgccgacgg
     1741 tccgcgcggc cttcttcggc ttcgacgtgg ccgacaggcc gaggtcgctc atcgcgtaga
     1801 tgatgcggcg ctgcacgggc ttcaggccgt cgccgagatg cggcagcgcg cgatcgagga
     1861 tcacgtacat cgagtagtcg aggtacgcct tctccgtgaa cgtcgcgagc ggcacccgct
     1921 cgatgccgtc gaaattcagc tcctggtctc tcatcccctt ctccaagagg tgccggtgtt
     1981 acggcttcgg ttcaaccgaa gtgccggagt tacggttcaa ccgaagtgcc ggagctacgg
     2041 ttgaaccgaa ccgtaa
//
LOCUS       DASSNN010000442         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_78965, whole genome shotgun sequence.
ACCESSION   DASSNN010000442 DASSNN010000000
VERSION     DASSNN010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78965"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFL84_08280"
     CDS             complement(<1..333)
                     /locus_tag="VFL84_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HET7608657.1"
                     /translation="MSQSIVTKLVLKDLWLAWPIVAGTLLLGVLSLALSPWSAVMFFV
                     GGSALIVALILLNIMLVTASLMSERKEKIRVFMLSMPVSTTQYFVSKRLSSLIGYLVP
                     AALLTVGGV"
     gene            complement(330..1226)
                     /locus_tag="VFL84_08285"
     CDS             complement(330..1226)
                     /locus_tag="VFL84_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7608658.1"
                     /translation="MSELAIQMAGVEKSYRYFNLRDIHLTLEPGQILGFVGPNGAGKS
                     TTIRILMALVRQDQGDVRVLGRSMPREQAIAKRDIGYMSEDMRLHGAASLAWHMKFVA
                     SVHPHWDHEYAQTLLRRFGLRAEQPIKVLSHGERTKATLLLVLARRPRLLLLDEPTTG
                     LDPVARHEILRELTDVMSDDRRSILFSSQNTQDVEQISDQITFLDRGRIIESSDKETF
                     VERWRRLQLDVPPGVALPSLPEVTEISASGRLATATIKAYTDDVAAAFTRAGATVRDV
                     QRMSLEEIFVANVMSSRKEVSA"
     gene            complement(1223..1654)
                     /locus_tag="VFL84_08290"
     CDS             complement(1223..1654)
                     /locus_tag="VFL84_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7608659.1"
                     /translation="MYTVHTDMHSELTISHADRRPMYLQIIEQIRHRVAIGDWKPGHE
                     LPSIRALAVATRVSVITVKRAYLELERDGVIVTRQGKGSIVAENVDLGTQLKHEELSQ
                     HLTAAAEIGKHLGLTTDELVDRLRETAGPTAGERQSDEEVA"
     gene            complement(1874..>2126)
                     /locus_tag="VFL84_08295"
     CDS             complement(1874..>2126)
                     /locus_tag="VFL84_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608660.1"
                     /translation="DVAVTVDFLDSGDRTVAKAKAEAAELTFGMRLDLQAPLPSDAVR
                     LRIYSVQWRMDGGPAALMGPFRVPWEFGYLMADPAKIDR"
BASE COUNT          372 a          711 c          683 g          360 t
ORIGIN      
        1 cacgccgccg acggtcagga gcgcggccgg cacgagatac ccgatcaacg acgacagccg
       61 cttcgagacg aagtactgcg tcgtcgagac cggcatgctc agcatgaaca cgcggatctt
      121 ctctttgcgc tcggacatca ggctcgccgt cacgagcatg atgttcaaaa ggatcagcgc
      181 gacgatcaga gcgctgccgc ccacgaaaaa catgacggcg ctccagggcg acagcgcgag
      241 cgacagtacg ccgagcaaca gcgtgcccgc cacgatcggc cacgcgagcc aaaggtcctt
      301 caagaccaat ttcgtcacga tggactgact cacgcgctga cctccttgcg gctcgacatc
      361 acgttggcca cgaagatctc ctcgagcgac atccgttgaa cgtcgcgcac cgtcgcgccc
      421 gcgcgcgtga acgccgcggc gacgtcgtcc gtgtacgctt tgatggtcgc cgtcgcgagc
      481 cggccgctcg cgctgatctc ggtgacctcg ggcagcgacg gcaacgcgac gccgggcggc
      541 acgtcgagct gcagccgccg ccagcgctcg acgaacgtct ccttgtcgct cgactcgatg
      601 atccggccgc gatcgaggaa cgtgatctgg tcggagatct gctcgacgtc ctgcgtgttc
      661 tgcgacgaga acaggatcga gcgccggtcg tcgctcatca cgtccgtgag ctcgcgcagg
      721 atctcgtgac gcgcgacagg gtcgaggccc gtcgtcggct cgtcgagcag cagcaggcgc
      781 ggccggcgcg cgagcacgag caggagcgtc gccttggtgc gctcgccgtg cgacagcacc
      841 ttgatcggct gctcggctcg gaggccgaac cggcgcaaca gcgtctgcgc gtattcgtga
      901 tcccaatgcg gatgcaccga cgcgacgaac ttcatgtgcc acgcgagcga cgcagcgccg
      961 tgcaggcgca tgtcctcgga catgtagccg atgtcgcgct tcgcgatcgc ttgctcgcgc
     1021 ggcatcgacc ggccgagcac gcgcacgtcg ccttggtcct ggcgcacgag cgccatcagg
     1081 attcggatcg tcgtcgactt gcccgcgccg ttcggcccga cgaagcccaa gatctggccg
     1141 ggctcgagcg taaggtgaat gtcgcgcagg ttgaagtagc ggtagctctt ctcgacgcct
     1201 gccatttgaa tcgcgagctc gctcatgcga cctcctcgtc actttgccgc tcgccggcgg
     1261 tcggccccgc ggtctcgcga aggcggtcga cgagctcgtc ggtcgtgagc ccgaggtgct
     1321 tgcctatttc ggcggcagcc gtgaggtgct ggctcaactc ttcgtgcttc aactgggtac
     1381 ccaagtcgac gttctccgcc acgatcgagc ccttcccctg cctcgtcacg atcacaccgt
     1441 cgcgctcgag ctcgagatac gcgcgcttca cggtgatcac gctcacgcgc gtcgcgaccg
     1501 ccagagctct gatcgacggg agctcgtgcc ccggcttcca gtccccgatc gcgacccgat
     1561 gccggatctg ctcgatgatc tggaggtaca tcgggcgccg gtcggcgtgg gagattgtga
     1621 gctcactgtg catgtcggta tgcacagtat acacggctat atacacagtg caaccgtcgc
     1681 cttgcacagc cgcgaaagcc actgccggcg cgggatgtag gcggtttcgc ctcgagcgca
     1741 gtgatataca cagtgagggg gggctgggcg cggttggaga cccgacgcgc gaccgaagga
     1801 agcggcccag cgggttcagc gattcatatc gacagacgta gagattgcaa cgcccgccga
     1861 gcaggagcct tgtttaacga tcgatcttgg cagggtcggc catcagataa ccgaattccc
     1921 aaggtacgcg gaacgggccc atcaacgccg ccgggccgcc gtccatgcgc cactgcacgg
     1981 aatagatgcg cagcctgacg gcatcggacg gtagcggcgc ttgcaggtcg agtcgcatgc
     2041 cgaacgtcag ctcggcagct tccgctttgg ccttcgccac ggtgcggtcc ccactgtcga
     2101 ggaagtccac ggtcacggcc acgtcg
//
LOCUS       DASSNN010000443         4052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_819677, whole genome shotgun sequence.
ACCESSION   DASSNN010000443 DASSNN010000000
VERSION     DASSNN010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819677"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VFL84_08300"
     CDS             complement(<1..881)
                     /locus_tag="VFL84_08300"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7608661.1"
                     /translation="MLGNLLHDVRYALHGFASRPMFAVVVVLTLAVGIGVNVAVFSLF
                     DRIMLRELNVANPHELVKLVSPGPRAGNQLGNQQGTDDETFSYPLFRELEAAGEPDVD
                     LAASWIAQVSLGNGDRTVRGSAVLVSGDYFSVLGVGPELGRVLAEQDVVDAEPAAAVV
                     LSFEYWTTAFGADPAVLGKTLLVAGQPLEVVGVAPRGFVGTTPGNKSNVFAPLTLDWY
                     RDAQLPNPIVADRFFTYLYVFGRLRPGASLDAVRAKLDVTFRAIINDVEAPQIAARSV
                     AAGREFQEEAEYRARTLS"
     gene            complement(884..2128)
                     /locus_tag="VFL84_08305"
     CDS             complement(884..2128)
                     /locus_tag="VFL84_08305"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET7608662.1"
                     /translation="MDIPRPENKKRKRIRQIVIGSGTAVALAIVTIALSRLEPAAPSV
                     DRDTLYTGKVERGEMLRQTRGPGTLVPRTRLWIAAQSAGRVDRVLVRPGAMVQPDTVL
                     VELSNPDLMRQAEEKRYGLEIAKANFAEFELGLRSKELDQKAAVATAYASYEGARLDA
                     EAKRASGAVAELDVRRSELNAQSLKTSYDIQVERLNQLAATVEAQVAAQRAKLAQEQN
                     TYERMREQVEALHVRASLAGVVQTVLVEEGAQVQPGANIARVAKPDELMAELKVPETQ
                     AKDVVPAQLVAVDTRNGIVSGKVIRVDPVASEGTVQVDVELTGKLPPGARSEQSVDGT
                     IEIERLPDVIYTGRPTYVQPNSTITLFKLVDEGAYAVRVPVAVGVTSVNQVEIREGLV
                     PGDEVILSDTSAWDDVDRIRLN"
     gene            2313..2546
                     /locus_tag="VFL84_08310"
     CDS             2313..2546
                     /locus_tag="VFL84_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7608663.1"
                     /translation="MIRLNHLALLVSSYRRSRDWYTANLGLELEFEIPERNTVALQDD
                     GGLTLFLSEPAAGAVGATCTLAFEVDDVHATYR"
     gene            2730..4016
                     /gene="tyrS"
                     /locus_tag="VFL84_08315"
     CDS             2730..4016
                     /gene="tyrS"
                     /locus_tag="VFL84_08315"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET7608664.1"
                     /translation="MHILDELAARGLVADVTDRDGLKKLLADERVTFYVGYDPTSPSL
                     HVGNLVPMIVQARLQRAGHKPIVLIGGATGMVGDPSGKSAERNLLGDAELEANIAGMR
                     SQLSRFLDFGAGPTGAVLVNNADWTRGVGYLEFLRDVGKYLTINYMMAKDSVRSRLES
                     EAGISYTEFSYMLLQAFDFVHLAKAYGCRLQAGGSDQYGNITAGCELSRKMGGPQLFG
                     LTAPLLLDSSGQKMGKTSTGERVWLDAERTPPYSFYQYFLNVTDEDAARLLKIFSLRP
                     LAEIDELVRSHDADRSQRLAQRELARELTTWVHGAEETARVEQASRIMFGGTLEGVEE
                     RTLELLTKVVPVVEIDRKELENGIALIELLTRTVAESKTAARRLVQQGGAYINNVRVT
                     EGERKIGLGDLVTPTLLVVRGGRKDYRLVRAAASAK"
BASE COUNT          693 a         1353 c         1332 g          674 t
ORIGIN      
        1 gagagagtcc tcgcacggta ttccgcttct tcttggaatt cgcgccccgc cgccacgctc
       61 cgcgccgcta tctggggtgc ctcgacgtcg ttgatgatcg cgcgaaacgt tacatcgagc
      121 tttgctcgca ccgcatcgag agacgcaccc ggccgcaacc tgccgaacac gtacaaatag
      181 gtgaagaatc ggtccgcgac gatcggatta gggagctgcg cgtcgcgata ccaatcgagc
      241 gtgagcggcg cgaacacgtt ggatttgttg ccgggcgtcg tgccgacgaa gccgcgcggc
      301 gccacgccga cgacctcgag cggctggccg gcaacgagga gcgtcttgcc gagaacagcc
      361 gggtcggccc cgaacgccgt cgtccaatac tcgaacgaca acacgacggc cgcggccggc
      421 tccgcgtcga cgacgtcttg ctctgcgagg acgcggccca actccgggcc aacgccaagc
      481 acagaaaaat agtcgcccga cacgagcacc gccgagcctc gcacggtgcg gtcgccgtta
      541 ccgagcgaca cctgcgcgat ccagcttgcc gcgagatcga cgtcgggctc accggccgcc
      601 tcgagctcgc gaaacagcgg atagctgaac gtctcgtcgt ccgtcccctg ttggttgccg
      661 agctggttgc cagcgcgcgg accggggctc acgagtttca cgagctcgtg cggattcgcg
      721 acgttgagct cgcgcagcat gatgcggtcg aacagcgaga acacggcgac gttgacgcca
      781 atgccgaccg cgagcgtcag cacgacgacg acggcgaaca tcggccgaga tgcaaacccg
      841 tgcagcgcgt agcgaacgtc gtgcagcagg tttccgagca tcgtcagttc agccggatgc
      901 gatccacgtc gtcccacgcc gaagtgtcgg aaaggatgac ctcgtcgccc ggcacgaggc
      961 cctcgcggat ctcgacctgg ttcaccgagg tgacgccgac cgcgacgggc acgcgcacgg
     1021 cgtacgcgcc ttcgtcgacg agcttgaaca gcgtgatcgt cgagttcggc tgcacgtaag
     1081 tcggccggcc cgtgtagatg acgtcgggca gccgctcgat ctcgatcgtg ccgtcgacgg
     1141 actgctcgga gcgcgcgccg ggcggtagct tgcccgtgag ctcgacgtcg acctgcaccg
     1201 tgccttcgga ggcgaccggg tcgacgcgga tgactttgcc cgaaacgatg ccgttacgcg
     1261 tgtctacggc gacgagctgc gccgggacga cgtctttcgc ttgcgtctcc ggaaccttga
     1321 gctcggccat cagctcgtcg ggcttcgcca cgcgcgcgat gttggcgccg ggctggactt
     1381 gcgcgccctc ttcgaccaat accgtctgca cgacgccggc gaggctcgcg cgcacgtgca
     1441 acgcttcgac ctgctcgcgc atgcgctcgt aggtgttttg ctcttgagca agcttcgcgc
     1501 gttgcgcggc gacttgggct tcgaccgttg cggcgagctg attgaggcgc tcgacctgaa
     1561 tgtcgtacga cgtcttcaac gattgagcgt tgagctccga ccttcggacg tcgagctcgg
     1621 ccacggcgcc ggacgcgcgc ttggcttcgg cgtcgagccg cgcgccttca tacgacgcat
     1681 acgccgttgc gacagccgcc ttttgatcga gctccttgct gcgcagaccc agctcgaact
     1741 ccgcgaagtt cgctttcgcg atctcgagcc cgtagcgctt ctcctcggcc tggcgcatga
     1801 gatcgggatt ggaaagctcg acgagaaccg tatcgggctg caccatcgcg cccggacgca
     1861 cgaggacgcg gtcgactcgg cccgccgact gcgccgcgat ccagagccgc gtgcgcggca
     1921 cgagcgtgcc ggggccgcgt gtctgccgca gcatctcgcc gcgctcgacc ttgcctgtgt
     1981 agagcgtgtc ccggtcgacc gagggagcgg ccggctcgag tcgcgacaga gcgatcgtga
     2041 cgatggcgag agccaccgcc gtgccgctgc cgatgacgat ttgccggatc cgtttgcgtt
     2101 ttttgttttc tgggcgtggt atgtccattt gccgcctcca gagcaacgaa cgtgccacgc
     2161 gagtgtacgc caattaactg cttggttttc aatcggaaac gcctgaagcg acgacgccga
     2221 gtgagcgccg gttcgtcccg cccgcggggc ccacgtcccg aaaacgggac gaaccgcaag
     2281 cagctacaat cgtccgcccg gacagcgaca gcatgattcg cctcaatcac ctcgccttgc
     2341 tcgtatcgag ctatcgccgc tcgcgcgact ggtacacggc caatctcggc ctcgagctcg
     2401 agttcgagat cccggagcgc aacaccgtcg cgctccagga cgacggtggg ctgactctgt
     2461 tcttgtcgga gccggccgcg ggcgccgtcg gcgcgacgtg cacgttggcg ttcgaggtcg
     2521 acgacgtcca cgcgacgtat cgctagctcg cggcgcgcgg cgtcgcattc gagaagacgc
     2581 cgcagaagct cttctggggc tacggcgccg agctgcgcga tcccgacggc tatctcatct
     2641 atctctggga cgaggaatcg atgcgcgaga aaggcgcatg agctgcgcgc agcgcccgcc
     2701 gcgcctgccg atcgggtaat cttcgcgcca tgcacattct ggacgagctg gccgcgcgcg
     2761 gcctagtggc ggacgtcact gaccgggacg gcctcaaaaa gttgctcgcc gacgagcgcg
     2821 tgacctttta tgttggctac gacccgacgt cgccgagcct gcatgtcggc aacctcgtgc
     2881 cgatgatcgt gcaagcgcgg ctgcagcgcg ccgggcacaa gccgatcgtg ctgattggcg
     2941 gcgccaccgg catggtcggc gatccgtcag gcaagagcgc cgagcgcaac ctgctcggcg
     3001 acgcggagct cgaggcgaat atcgccggca tgcgctcgca gctatcgcgg ttcctcgatt
     3061 tcggcgcggg ccccacgggc gccgtgctcg tcaacaacgc ggactggacg cgcggcgtcg
     3121 gttacctcga gttcctacgc gacgtcggca agtacctgac gatcaactac atgatggcta
     3181 aggactcggt gcgctcgcgc ctcgagagcg aagccggcat cagctacacc gagttctcgt
     3241 acatgctgct gcaagcgttc gatttcgtgc atttggcgaa ggcctacggc tgccggttgc
     3301 aagccggcgg ctccgatcaa tacggcaaca tcacggccgg ctgcgagctc tcgagaaaga
     3361 tgggcggccc gcagctcttc gggctcacgg cgccgctgct gctcgactcc tcgggccaga
     3421 agatgggcaa gacgtcgacg ggcgagcgcg tctggctcga cgccgagcgc acgccgccct
     3481 actcttttta tcaatacttc ttgaacgtga cggacgagga cgccgcgcgc ctgctgaaga
     3541 tcttctcgct gcggccgctc gcggagatcg acgagctggt ccgctcgcat gacgcggatc
     3601 gaagccaacg cctcgcacag cgcgagctcg cgcgcgagct tacgacgtgg gtgcacggag
     3661 cggaggagac ggcgcgcgtc gagcaagcaa gccggatcat gttcggcggc acgctcgaag
     3721 gcgtcgagga gcgcacgctc gagctactga cgaaggtcgt gcccgtcgtc gagatcgacc
     3781 gaaaggagct cgagaacggc atcgcgctga tcgagctctt gacgcggacc gtcgccgagt
     3841 cgaagacggc ggcacggcgg ctcgtgcagc aaggcggcgc atacatcaac aacgtccgcg
     3901 tcacggaagg cgagcgcaag atcggcctcg gcgacctcgt cacgccgacg ctgctcgtcg
     3961 tgcgcggcgg gcgaaaagac taccggctcg tccgcgctgc ggcctccgcc aagtaaacgt
     4021 aacgagcgta acggttgtcg cggccccgcg cc
//
LOCUS       DASSNN010000444         3583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_941516, whole genome shotgun sequence.
ACCESSION   DASSNN010000444 DASSNN010000000
VERSION     DASSNN010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941516"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..592)
                     /locus_tag="VFL84_08320"
     CDS             complement(65..592)
                     /locus_tag="VFL84_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HET7608665.1"
                     /translation="METRSVLSRWIAVAMVAAALSACGGPDDDLRAYIDEVKARPGGR
                     IEPLQPYQPAPTFAYEAGTRRSPFVPEAPQRRRSNDPNAVEGPDQNRPREFLEQFPLD
                     TLRMVGTLADRRASFGLVQTNDGLVHRVTVGNHMGQNYGRVVSISDSQIQLVEIISDG
                     LGGYLERPAAIGLSD"
     gene            complement(592..1197)
                     /locus_tag="VFL84_08325"
     CDS             complement(592..1197)
                     /locus_tag="VFL84_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HET7608666.1"
                     /translation="MSIVDELRSLDTNDPGRWPLPIRVGAVALVFGAAVAAGIWITVI
                     KAEMPRLEQAQATERELRGQFEDRQRKAANFDAFRAQLAEIERDFGAMLRQLPGKTEV
                     PSLLVDISQTGLGAGLEEQTFTPMGEIQKDFYAELPIKLRYTGSYHELGNFVSGIAAL
                     PRIVTLHDISIRPVNSSNPDELTLDVTAKTYRYLDEEAGEG"
     gene            complement(1194..1787)
                     /locus_tag="VFL84_08330"
     CDS             complement(1194..1787)
                     /locus_tag="VFL84_08330"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HET7608667.1"
                     /translation="MPRINLLPWRAELRQKRKKEFFVALMGSLIVAAGTVYLSKLTVK
                     GWTSAQENRNQVLRTEIAALDSQIAEIKTLETQRDRLKARMQIITQLQRSRPEVVHLF
                     DELVTAVPEGVYFTEVTQQGTHIEVKGNAQSSTRVSALMRNIDDSAWLRGPGLEGVDF
                     VTAGTERNAQFRVFAEQVPMTSPDDEVAQAAPQGALR"
     gene            complement(1787..2848)
                     /locus_tag="VFL84_08335"
     CDS             complement(1787..2848)
                     /locus_tag="VFL84_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HET7608668.1"
                     /translation="MFATSRKGSLVGLDITTSSVKLIELVTSGRGYRVEAYAAEPTPP
                     NAINEKAIVDAEAVGEAIRRAHKRAGTKTREAAIAISGDAAITKVIQMPKNLSSGDLE
                     GQVELQADQYIPFPMEEVSFDFEVIGDSERDPDMVDVLLVATRTENVEQRQVAVEAAG
                     LQAKVVDVEAFAVENACQLLTHQMPDAGADKFIAIVDFGASTTTFSVLKDLKVIYTRD
                     FAFGGQQLTEEIMRTYGLSMEDAGRAKKEGGLPSNYQPEVLDAFIDDMSQQVSRSLQF
                     FLASGSGREQPDQILICGGCANIPGIADVISSRVGIPTEIGDPLGQMKISGRAKSQGV
                     DRDATALLTACGLALRSFD"
     regulatory      complement(3135..3231)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3310..>3583
                     /locus_tag="VFL84_08340"
     CDS             3310..>3583
                     /locus_tag="VFL84_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET7608669.1"
                     /translation="MTILALAVGFALVVSGLLIGVYYYVAPGLPQAAELRDIRIQVPL
                     QVYSRDGRLIDEFGEQKRTPVAYEHIPPLLIKAVLAAEDEHFFEHPG"
BASE COUNT          587 a         1155 c         1126 g          715 t
ORIGIN      
        1 aggttctcgc gacggacggg cactgacata ttcatacggt taactcccta agctcccctg
       61 cgcttcaatc ggacaggcca atcgcggcgg gacgttccaa gtacccgccg aggccgtcgg
      121 agatgatctc caccaattga atctgagaat ccgaaatgga aacgacgcgg ccgtagttct
      181 ggcccatgtg gttgccgacg gtgactcgat gcacgagccc gtcgttcgtc tgaactagac
      241 cgaaactggc gcgccgatcg gcgagcgtgc cgaccatgcg cagagtatcg agcggaaact
      301 gctcgagaaa ctcgcgcggc cgattctgat ccgggccttc gaccgcgttc ggatcgttgc
      361 tgcggcgccg ctgcggcgcc tcgggaacga acggcgagcg gcgcgtgccg gcctcgtacg
      421 cgaacgtcgg cgccggctga tacggctgca atggctcgat gcggccgccg ggccgcgcct
      481 tgacctcgtc gatataggcg cgcaaatcgt cgtcggggcc gccgcaggcg ctcaaagccg
      541 cggcgaccat ggccaccgcg atccagcgtg agagtacgct acgcgtctcc atcaaccctc
      601 ccccgcttcc tcatccaggt agcgataagt cttcgccgtc acgtcgagcg tcagctcgtc
      661 ggggttcgag ctgttgacgg ggcgaataga gatgtcatgt aaggtcacaa tgcggggcag
      721 cgcagcgatg ccgctcacaa aattgcccag ctcgtggtag ctgcccgtgt agcgcagctt
      781 gatcgggagc tccgcgtaga agtccttttg gatctcgccc atcggcgtga acgtctgctc
      841 ctcgaggccc gcgccgaggc cggtttgcga gatgtcgacg agcaagctcg gaacctcggt
      901 tttgcccggt agctggcgca gcatcgcgcc gaagtcgcgc tcgatctcgg ccagctgcgc
      961 gcggaaggca tcgaagttcg cggccttgcg ctgccgatcc tcgaactggc cgcggagctc
     1021 gcgctccgtc gcctgggctt gctcgagcct cggcatttcg gccttgatga cggtgatcca
     1081 gatgccggcg gccaccgccg ccccaaacac gagcgcgacg gcgccgacgc ggatcggcag
     1141 cggccaacgg ccgggatcgt tggtatcgag tgagcgaagc tcgtcgacga tgctcatcgc
     1201 agtgctcctt gcggtgccgc ttgcgcgacc tcgtcgtcag gcgacgtcat cggcacttgc
     1261 tccgcgaata ctctgaattg cgcattgcgc tccgtgcccg ccgtcacgaa gtcgacgccc
     1321 tcgagaccgg gcccgcgcag ccatgcggaa tcgtcgatgt tgcgcatcaa cgccgagacg
     1381 cgcgtgctgg attgggcatt gcccttcact tcgatatgcg tgccctgctg cgtgacttcg
     1441 gtgaagtaga cgccctctgg cacggcggtg acgagctcgt cgaacaagtg cacgacctcg
     1501 gggcgcgagc gctgcagctg cgtgatgatc tgcatgcgcg ccttgaggcg gtcgcgctgc
     1561 gtctcgagcg ttttgatctc cgcgatctga ctgtcgagcg cggcgatctc cgtacgcagg
     1621 acctggttgc ggttctcctg cgcgctcgtc caacccttga ccgtcaactt ggacagatag
     1681 acggtgccgg ctgcgacgat gaggctgccc attagggcca cgaagaattc tttcttgcgc
     1741 ttctggcgca gctctgcgcg ccagggcagt aggttgattc tcggcattag tcgaagctcc
     1801 tcaaggcgag accgcacgcg gtcaacagcg ccgtggcgtc tctatccaca ccctgcgact
     1861 tggcgcggcc cgaaatcttc atctgcccga gcggatcgcc gatctccgtc ggaatcccga
     1921 cccggctcga gatcacgtcc gcgatgccgg ggatgttcgc gcagccgccg cagatcagaa
     1981 tctggtccgg ctgctcgcgg ccgctgcccg acgccaagaa gaactgcagc gagcggctga
     2041 cttgctgcga catgtcgtcg atgaaggcgt cgagcacctc cggttgatag ttgctcggca
     2101 ggccgccttc cttcttcgcg cggcccgcgt cctccatgct caacccgtac gtccgcatga
     2161 tttcctcggt gagctgctgc ccgccgaatg cgaagtcgcg cgtgtagatg actttgagat
     2221 ccttgagcac gctgaacgtc gtcgtgctcg cgccgaagtc gacgatcgcg atgaacttgt
     2281 cggcgccggc gtccggcatc tggtgcgtca ggagctggca cgcgttctcg accgcgaacg
     2341 cttcgacgtc gacgaccttc gcttgcaggc cggccgcctc gacggcgact tggcgctgct
     2401 cgacgttctc cgtgcgcgtc gcaacgagca gcacgtcgac catgtcggga tcgcgctcgc
     2461 tgtcgccgat gacctcgaag tcgaagctga cttcttccat cgggaacggg atgtattgat
     2521 cggcttgcag ctcgacctga ccctcgaggt cgcccgagct caggttcttc ggcatctgga
     2581 tgactttcgt gatcgcggcg tcgccgctga tcgcgatcgc ggcctcgcga gttttcgtgc
     2641 cggcgcgctt gtgcgcgcgg cgaatcgctt cgccgacggc ctcggcgtcg acgatggctt
     2701 tctcgttgat cgcgttcggc ggcgtgggct cggcggcgta cgcttcgacg cgataaccgc
     2761 gtccgctcgt gacgagctcg atgagcttga ccgacgaggt ggtgatgtcg aggcctacca
     2821 aggatccttt gcgactcgtg gcgaacacgc tcttttcctt tccaacaccg gaggttgggt
     2881 tagaatccta caggtgcctt caactgccca atttaactat atctcacccg agatatacac
     2941 cgttgtgacg ggtctctcga tccgtcaatc gagcctaaac ccgtcgcgcg agcgtagccg
     3001 tcgcggaaac tgccggctgt gaccggtttc cctacttggc cgggacgagc gttcggtgtc
     3061 gcgggtgggt cgcggagaaa cggttggcga agcgtcgagt cggttccaaa aacgacagct
     3121 cgattttcta ctgcttcggc aaccccgcta tcctagttgc ggctcgagtg ccgcgcccgt
     3181 agaccggagg ctttgcgtcc ccacctttcg atgggtttgc cttgacgcgg cggactatgc
     3241 acaaaggcct gaggtgtggc aagggcgtac aatgcaactt cgccgatgaa atctacttca
     3301 cagattttgt tgacaattct ggccctcgcg gtgggcttcg cgctggtcgt ctccggacta
     3361 ttgatcggcg tctactacta cgtcgcgccg ggtttgccgc aggcggctga gctgcgcgac
     3421 atcaggatcc aggttccgct tcaggtctat agccgcgacg gccggctcat cgacgaattc
     3481 ggcgagcaga agcggacccc ggtcgcctac gagcacattc cgccgctgct catcaaggcc
     3541 gttctcgccg ccgaggacga gcacttcttc gagcaccccg gca
//
LOCUS       DASSNN010000445         3307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_676947, whole genome shotgun sequence.
ACCESSION   DASSNN010000445 DASSNN010000000
VERSION     DASSNN010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676947"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VFL84_08345"
     CDS             <1..741
                     /locus_tag="VFL84_08345"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET7608670.1"
                     /translation="MTDTNLHNKIALVTGGGRGIGNAIALALAEEGCDVAVNYVSREA
                     DARQTAEAIEALGRRSLVVRGDVAKSADVAALVAAVEKQLGPVDILVNNAGRAAFESL
                     EQMTEASWNDTIQVNLTSVFLMTQAVLPGMRARKWGRVINLTSVAAQAGSVLAVHYSA
                     AKAGVIAATKSYARLLAKEGVTVNAISPALIGTEMVASNPHIKPDMIPVGRFGRVEEC
                     ADVAVLLARNGYITGQTISVNGGMYFAA"
     gene            complement(757..1935)
                     /locus_tag="VFL84_08350"
     CDS             complement(757..1935)
                     /locus_tag="VFL84_08350"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7608671.1"
                     /translation="MRTRVSVAALALFLSPSFLAAQVPGVPERPLPDKPFVIDTAEAG
                     PVRVTPLKGLELPWDLAFLPSGDMLVTERTAMRLRLVRKGVLEPAPIAGLPERMSAGG
                     GGGLMAVAVHPRFAQTGWVYLTYTKPAERGRRGLGLARGRLDGMALKDVRELFTVDES
                     GGGPPPGLPLVFAPDGYLYFFIGGANDMIAQRGDSYYGKVLRFNDDGSVPKNPFAGRD
                     DVAKEIFTIGHRNGIGLTVHPTTGEIWESENGPYGGDEVNILKPGANYGWPLVSYGRD
                     YSGAPVSDKRTLPEVEDPVLFWVPSIAISGMTFYTGDRFPAWRNNLFVGGLQYGRLPR
                     SGRMHRVVFNEQWQEVRREAFFEDLRQRIRDVEQGPDGLLYVLTDERDGAVLKLEPAE
                     "
     gene            2071..3036
                     /locus_tag="VFL84_08355"
     CDS             2071..3036
                     /locus_tag="VFL84_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006176802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608672.1"
                     /translation="MSAIVEPTRPYVDRDDLAPVASRSAVSWGAVFAGAAAAAALSLI
                     LLLLGTGLGLSSISPWNAEGIDAGTFGVSSILWITFMQLAASALGGYLAGRLRTKWAG
                     VNRDEVYFRDTAHGLLAWAVATLGTAALLSSVVGAIVAGGVQAAGAAVGNAASAAVSG
                     ATVAGTQAEPGQAGQAGQAGAAEGPMAYFVDSLFRAGAGDDLRDDRPPEQSAASVAEV
                     TRIFANGIRMGELPNEDVQYVARVVAQRTDLSQQQAEQRVREVFMKMQMTLNEAETQA
                     REAADAARKASAYASLWLFISLLAGAFVASWMATFGGRQRDLYEA"
BASE COUNT          520 a         1092 c         1154 g          541 t
ORIGIN      
        1 atgactgata cgaaccttca caacaaaatc gcgctcgtga ccggcggcgg gcgcggcatc
       61 ggcaacgcca tcgcgctggc gctggccgaa gagggctgcg acgtcgcggt caactacgtc
      121 agccgcgagg ccgacgcgcg gcagaccgcc gaggcgatcg aagctctcgg aaggcgcagt
      181 ctcgtcgtgc gcggcgacgt ggcgaagagc gcggacgtcg cggcgctcgt cgcggccgtg
      241 gaaaagcagc tcgggcccgt cgacatcctc gtcaacaacg ccggacgcgc ggcgttcgag
      301 agcctcgagc aaatgaccga ggcgagctgg aacgacacga ttcaagtcaa cctcacgtcg
      361 gtgttcctga tgacgcaggc cgtgctgccc ggcatgcgcg cgcgcaagtg gggccgcgtc
      421 atcaatctga cgtcggtagc cgcgcaggcc ggcagcgtct tggcggttca ttactcggcc
      481 gccaaggccg gcgtcatcgc cgcgacgaag agctacgcgc ggctgctcgc gaaggaaggc
      541 gtcaccgtca acgcgatctc gccggcgttg atcggcacgg agatggtcgc gtcgaacccg
      601 cacatcaagc ccgacatgat cccggtcggg cgcttcggcc gcgtcgagga gtgcgccgac
      661 gtcgcggtgc tgctcgcgcg caacggctac atcacgggcc agacgatctc cgtcaacggc
      721 ggcatgtact tcgccgcgta gccgacggcc gccccgtcac tcggccggct cgagcttcag
      781 cacggcgccg tcgcgctcgt cggtcagcac gtaaagaagc ccgtcgggcc cttgctcgac
      841 gtcgcggatg cgctgccgca ggtcctcgaa gaacgcttcg cggcgaactt cctgccactg
      901 ctcgttgaag acgacgcggt gcatacggcc gctgcgcgga aggcggccgt actgtaagcc
      961 gccgacgaag aggttgttgc gccacgcggg gaagcgatcg ccggtgtaga acgtcatgcc
     1021 ggagatcgcg atcgacggca cccagaacag cacgggatct tcgacctcgg gcagcgtgcg
     1081 cttgtcggac acgggcgcgc cgctgtagtc gcggccgtag ctgacgagcg gccagccgta
     1141 gttcgcgccg ggcttcaaga tgttgacctc gtcgccgccg tagggaccgt tctcgctctc
     1201 ccagatctcg ccggtcgtcg gatgaaccgt gaggccgatg ccgttgcgat ggccgatcgt
     1261 gaagatctcc ttggcgacgt cgtcgcggcc ggcgaacgga ttcttcggca cgctgccgtc
     1321 gtcgttgaag cgcagcacct tgccgtaata ggaatcgccg cgctgcgcga tcatgtcgtt
     1381 ggcgccgccg atgaagaagt agagatagcc gtcgggcgcg aacacgagcg gcaatccggg
     1441 cggcgggccg ccgcccgatt cgtcgacggt gaagagctcg cgaacgtcct tcagcgccat
     1501 tccgtcgagg cgcccgcgcg ccagtcccaa gccgcgacgt ccgcgctcgg ccggcttcgt
     1561 atacgtcagg tacacccagc ccgtctgcgc gaaccgtggg tgcacggcca cggccatgag
     1621 cccgccgccg cctccggcgc tcattcgctc gggcaggccc gcgattggcg ccggctcgag
     1681 cacgcctttg cgcacgaggc gcaagcgcat cgcagtgcgc tccgtgacca gcatgtcgcc
     1741 gctcggcagg aacgcgaggt cccagggcag ctcgagacct ttcagcggcg tcacgcgcac
     1801 tgggcccgct tcggccgtgt cgatgacgaa cggcttgtcc ggtagtggcc gctcgggaac
     1861 gccgggtact tgcgcggcga gaaaggacgg cgagaggaaa agggctaggg cagcgaccga
     1921 aacgcgggtg cgcacgagcg ggactccgga tggatgcgtc gcgcgtcact gtactcgaac
     1981 gccgattgcg atgccctccg tggcgccgct gcgcgccctg gctcgtttct tgcaactaca
     2041 cgcgcagttt agatgcggag ggccgcccca atgagcgcca tcgtagaacc caccaggcca
     2101 tacgtagacc gtgacgacct cgcgcccgtc gcttcgcgaa gcgcggtgtc gtggggagcc
     2161 gtgtttgcgg gagcggccgc ggcggccgcg ctgtcgctga ttctgttgtt gctcggcacc
     2221 gggctcgggc tgtcgtcgat ttcgccgtgg aacgcggaag ggatcgatgc cggcacgttc
     2281 ggcgtctcga gcatcctctg gatcacgttc atgcagctcg cggcgtccgc gctcggcggc
     2341 tatctcgccg gaaggctgcg gacgaagtgg gcgggcgtga accgcgacga ggtgtacttc
     2401 cgcgataccg cgcacggcct gctggcgtgg gccgtcgcaa cgctcggcac tgccgcgctg
     2461 ttgtcgtcgg tcgtcggcgc gatcgtcgcc ggcggcgtgc aggcggccgg cgccgccgtc
     2521 ggcaatgcag cttcggccgc ggtcagcggc gccacggttg caggcacgca agccgagccg
     2581 ggccaggcgg ggcaggcggg gcaggccggc gcggccgaag gcccgatggc ttacttcgtc
     2641 gattcgctgt ttcgagccgg cgcgggcgac gatctgcgcg acgatcgacc gcccgagcaa
     2701 tcggccgcgt cggtcgccga ggtgacgcgc atctttgcga acggcattcg aatgggcgag
     2761 ctgcccaacg aggacgttca gtacgtcgcg cgagtcgtcg cacaacgcac cgatctctcg
     2821 cagcaacagg cggagcagcg tgtgcgcgaa gtgttcatga agatgcagat gacgctgaac
     2881 gaggccgaga cgcaggcgcg cgaggccgcc gacgccgcgc gcaaagcctc cgcgtacgcc
     2941 tcgttgtggt tgttcatctc gctgctcgcg ggcgcgttcg tcgcgagctg gatggccacg
     3001 ttcggcgggc gacagcgcga tctttacgaa gcttaggaga gaacgatgcg ttcgctattg
     3061 ctatggatcc tcggcgtgcc gctgccgatc atcatcctga tcgcgctgtt cacgcactga
     3121 gctaggcgcc tattgcgcgc gcttcgcgat gacttgcatc gcgggccccg tcgcgccttc
     3181 gacggcgcgc gtgaagtgga tctcttcgcc ctcgatcgtg ccttgccaga ggatcttgtt
     3241 cgtcgcgttg ttcgccgtgc gcaggacgtg aaacgaaatc ttgttgccct cgatcttgcc
     3301 gtctttg
//
LOCUS       DASSNN010000446         8041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_941849, whole genome shotgun sequence.
ACCESSION   DASSNN010000446 DASSNN010000000
VERSION     DASSNN010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941849"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VFL84_08360"
     CDS             <1..497
                     /locus_tag="VFL84_08360"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7608673.1"
                     /translation="GELWQMEIGPQGGDEVNILKPGANYGWPLVSLGRNYSGTLVSDQ
                     AFHRDGMEDPVIFWTPQISPAAMAFYTGDKFPQWQNSLIIGALSGQHVERMPFNDKGQ
                     PLRREEFLSELGVRFRDVEIGPDGYIYFSTEVRYGSGQPDGTVIRLEPAGDPLPAPTR
                     NRSN"
     gene            542..1279
                     /locus_tag="VFL84_08365"
     CDS             542..1279
                     /locus_tag="VFL84_08365"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET7608674.1"
                     /translation="MKRIALAAAVALPLFFGAANTAFAAKIAVIGTGNVGSALGPEFA
                     ALGHTIVYGSRTPNEKDVQDLVAKTGHGATATTQPEAVKGADMVLLAVPGNLAVDITK
                     SLGDLSGKIIIDPTNRYTRNTPDGYFTHDVPGGSNAELIQQAAPGAKVVKAFNTLNWT
                     KMVDPQSSGGPVSILIVGDDAAARAAVAELVKGMGLEPVDLGPLRFANTLEEMLVIWA
                     NARGRGAAYNYYLRPEPPSPRPSAPAR"
     gene            complement(1276..2586)
                     /locus_tag="VFL84_08370"
     CDS             complement(1276..2586)
                     /locus_tag="VFL84_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7608675.1"
                     /translation="MRRSLLAAALAALVGAAPAALAQQHSADTSASALHWAAYDNDLG
                     AARRLLAEGADPNAANRFGVTPLHEAATVGNAEMLKAMLEAGGNANAAFGEGETVLMT
                     AARAGNAESVRLLLAHGADVNASEHWHGQTALMWAAMENHADVVQILIDAGADVHARS
                     TKHDWVKISYSEGNVPKTRDIGGLTALQLAAREGSLESVEKLLDAGADASAAEPMYQL
                     TPLQLAIVNGHYTLAMRLLERGVGADDGSLYLAIDTRNLGFYAQRPNPPEKDGDVTSL
                     DVVNALLERGADPDAPYTKGLPEGILPEGDAKPRLPERTVAGGIAVPPGATPLDRAAA
                     AGDFAVVETLLEHGASASVATEDGTTPLMLLAGYSRTRTNAPLADIPKRLELIRLMLE
                     KGADVHAVHKDSTNNALFFAKQRNASEIVALLEQHGAREATAAN"
     gene            complement(2606..3982)
                     /locus_tag="VFL84_08375"
     CDS             complement(2606..3982)
                     /locus_tag="VFL84_08375"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET7608676.1"
                     /translation="MFISKKHLSRRTVLKGVGAAVALPLLDAMVPARTALAQTAAAPT
                     LRFGTIYIPHGAIMDVWMPKKVGTGFDLPVILKPLENLREHFNVVTGLYSEGANAHSA
                     CPGFFSACAHAPRGNLIELNTSIDQVIAQKIGQGTTFPSMELAIEDSSNIFGTCAADF
                     LCTYMDTISWRTPTQPLPMELNPRVVFERMFGGDGTTPEARRARLEQSTSILDAVASD
                     VRGLEAKLGTRDRARLDEYLANVREVERRIVQAEDQRSERRLEAPPTPSGIPESFEEH
                     VNLMFELQALAFQGDLTRVTSFMMGRELSGLSYPQIGISDGHHPVSHNNYDPKQMEKK
                     AKIDTYNVSLFAKFLQRLKDTPDGDSNLLENSLFVYGSGMSDGNVHNHLNLPVLLAGN
                     AGGRLKGGHHIQAGQLAKERAIPAIPKFDKMVPFANLMVSVLQLYGIETESYGKDMCA
                     SNGSVSLA"
     gene            complement(4055..6436)
                     /locus_tag="VFL84_08380"
     CDS             complement(4055..6436)
                     /locus_tag="VFL84_08380"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET7608677.1"
                     /translation="MPIVALALASVGCQPSVEQRAAAAKNLVDRYCTDCHNDAERTGE
                     LTLERLALTDVGAHADVWETVVKKLRGRMMPPAGEPLPEAAATDELVAYLEEQLDRAA
                     AEHPNPGTKSLHRLNRTEYGNAIRDLLALDIDASAYLPNDSEAYGFDNIADVLGTDPS
                     LLDRYLSAAWKITSAAMGDLSLAPAVATYRVPPDRSQRDHVDGLPLGTRGGMLVSHFF
                     PVDGEYVIRPKLWRNTVDVVRGTETPHDLEVSLDGAQLTLTRFGGPADEVPAQMFPGK
                     TADEIDRRFETRVVVSAGQHDVGVAFAKKSSAARQDVLQAFEREKHDARMDVGIPELD
                     QIVIEGPLTVAGPGRSPSRERIFSCFPKQESEAASCAEQILTALARRAYRRPITDNER
                     ERLLELYGAERAKGRTFEAGVQTGLAYILVSPQFLFRVEQDPVDAVPGSIYRIGDLEL
                     ASRLSFFLWNSLPDDELIDAAVAGKLGDSKELRAQVERMLADERATTLAKTFASQWLY
                     LRNLRASTPDMYVFPDFDDNLRQSAIRETELLFETIVREDRPLTELLSADYTYVNERL
                     AKHYGIPNIYGDQFRRIAQTDERRYGLLGHASVLTLSSYPNRTSPVSRGNYVLTNILG
                     TPAPQPPPNVPTLPENTAEKLSMRARMERHRADPVCASCHQLMDPIGLVLERFDGIGR
                     WRDEDNGEPVRDYGSPIHVLRDFGPINGPVDLRNAILSRPERFVRTATEKLMTYALGR
                     QLTTADMPTARAVVRKAAEDDYRFSSLVLGIVTSDTFQMRIAGERQAETIALD"
     gene            complement(6505..7647)
                     /locus_tag="VFL84_08385"
     CDS             complement(6505..7647)
                     /locus_tag="VFL84_08385"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7608678.1"
                     /translation="MATRTKPFHVATLALALAATAPALAHHGFGLFQMQEFKDYSGTL
                     TKLELVNPHSYLYFETVDADGKPLEMRCEMRAATLIRRSGWKIDEFVPGRHVDIHAHP
                     HRDDPHSCYLENVAIGGTTKIDRNDQFTHAPVDTSKRPLRLPSGEPNISGDWAVEQAV
                     LTIPPSGGNGSLVPRSLREDFAAGKITLEQIRARNPAPSRPQYTAKGEETSKAFRMWS
                     PEDNPRLSCKPTSIVFDWTFDWPINRITQTTVDGENVIDIDYGLFSASRRFHLGMTEH
                     PANLTPTNAGHSIGRWDGDTLVVDTVGFAEGVLVPPTRNSPQLHVVERFTLDPKTFAL
                     KREYTAEDPVYLAAPHKGQDVVLLSEVPFEKQPCSELTFEFTRPGN"
     gene            7754..>8041
                     /locus_tag="VFL84_08390"
     CDS             7754..>8041
                     /locus_tag="VFL84_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608679.1"
                     /translation="MTTIRRAPWALAVAAALLAQAGPGSRADAQSSADCDSLCSAAAE
                     LVERFELRESAAPVRERVGWERPKKIVVADVPLADYLRRAVPGVEIVGVAGL"
BASE COUNT         1230 a         2658 c         2779 g         1374 t
ORIGIN      
        1 cgggcgagct ctggcagatg gaaatcggcc cgcagggcgg cgacgaggtc aacatcttga
       61 agcccggcgc caattacggc tggccgctcg tttcgctcgg ccgcaactat tcgggcacgc
      121 tcgtctccga tcaggcgttc catcgcgacg gcatggaaga cccggtcatc ttctggacgc
      181 cgcagatcag cccggccgcg atggcgttct acacgggcga caagttcccg cagtggcaga
      241 acagcttgat catcggcgcg ttgagcggcc agcacgtcga gcgcatgccg ttcaacgaca
      301 agggccagcc gctgcgccgc gaggagttct tgagcgagct cggcgtgcgc ttccgcgacg
      361 tcgagatcgg gcccgacggc tacatctatt tctcgacgga agtgcgctac ggcagcggcc
      421 agcccgacgg cacggtcatt cggctcgagc cggccggcga tccgctgccc gcgccgacgc
      481 gcaaccgaag caattgacgc aacgtccgat ccaagtcgag agaaacgaaa ggagaaccgt
      541 catgaagcga atcgcgctcg cggccgccgt tgcgttgccg ctgttcttcg gcgccgcgaa
      601 cacggcgttt gcggcgaaga tcgccgtcat cggcaccggc aacgtcggct cggcgctcgg
      661 gcccgagttc gcggcgctcg gccacacgat cgtttacggt tcgcgtacgc cgaacgagaa
      721 agacgttcaa gatctcgtcg cgaagaccgg tcacggcgcg acggcgacga cgcaaccgga
      781 agccgtcaaa ggcgccgaca tggtgctgct cgccgtgccc ggcaacctcg ccgtcgacat
      841 cacgaagagc ctcggcgacc tgtccggcaa gatcatcatc gacccgacga accgttacac
      901 ccgcaacacg ccggacggct acttcacgca tgacgtgccg ggcggcagca acgcggagct
      961 catacaacag gccgcgccgg gcgccaaggt cgtgaaggcc ttcaacactt tgaactggac
     1021 gaagatggtc gacccgcaga gctcgggcgg ccccgtgtcg attctgatcg tcggcgacga
     1081 cgcggctgcg cgcgccgcgg tggccgagct cgtcaaaggc atgggcctcg agcccgtgga
     1141 tctcgggccg ctgcggttcg cgaacacgct cgaggagatg ctcgtgatct gggcgaacgc
     1201 gcgcggtcgc ggcgcggcct acaactacta cttgcggccg gagccgccga gcccgcgacc
     1261 gagcgctccc gcgcgctagt tcgcggccgt cgcctcgcgc gcgccgtgct gctcgagcag
     1321 cgcgacgatc tcgctcgcgt tgcgctgctt cgcgaagaag agcgcgttgt tcgtcgagtc
     1381 cttgtgcacg gcgtgcacgt cggcgccctt ctcgagcatg agccggatca gctcgaggcg
     1441 cttcggaata tccgcgagcg gcgcgttggt gcgagtgcgc gagtaaccgg cgagcagcat
     1501 cagcggcgtc gtgccgtcct cggtcgcgac cgaagcgctc gcgccgtgct ctagcaacgt
     1561 ttcgactaca gcgaagtcgc ccgcggccgc cgcgcggtcg agcggtgttg cgccgggcgg
     1621 cacggcaatt ccgccggcga ccgtgcgttc cgggagccgc ggtttggcat cgccctcggg
     1681 gaggatgccc tcgggaagcc ctttcgtgta cggcgcgtcc ggatcggcac cgcgctcgag
     1741 caatgcgttc acgacgtcga gagaagtcac gtcgccgtct ttctcgggcg gattcggccg
     1801 ctgcgcgtag aagcccaggt tgcgcgtgtc gatggcgaga tacagagagc cgtcgtcggc
     1861 gccgaccccg cgctcgagca gccgcatcgc gagcgtgtag tgcccgttga cgatcgcgag
     1921 ctgcagcggc gtgagctgat acatcggctc ggccgcgctc gcgtcggcgc ccgcgtcgag
     1981 cagcttctcg acggattcga gcgagccttc gcgcgccgcg agctgcagcg ccgtgaggcc
     2041 gccgatatcg cgcgtcttcg gcacgttgcc ttcggagtag ctgatcttga cccagtcgtg
     2101 cttcgtcgag cgcgcgtgca cgtcggctcc cgcgtcgatc aagatctgca cgacgtcggc
     2161 atgattctcc atcgcggccc acatcagcgc cgtttggccg tgccagtgct cggacgcatt
     2221 cacatctgcg ccgtgcgcga gcaagagccg cacgctttcg gcgttaccgg cgcgcgcggc
     2281 cgtcatcagc acggtttcgc cttcgccgaa ggctgcgttc gcgttgccgc cggcctcgag
     2341 catcgccttc agcatctcgg cgttgccgac ggtcgccgcc tcgtgcagcg gcgtcacgcc
     2401 gaaacggttc gccgcgttgg gatcggcgcc ttcggcgagc aaccgccgcg cggcgccgag
     2461 gtcgttgtcg tacgcggccc agtgcagcgc cgacgccgac gtgtcggccg agtgctgctg
     2521 cgcgagggcc gcgggcgccg cgccgaccag cgctgcgagc gccgctgcca agagggatcg
     2581 tctcaccgcc cggcctcgcg cggcgttagg ccagcgagac gctgccgttg ctcgcgcaca
     2641 tgtctttgcc gtagctctcg gtctcgatgc cgtagagctg cagcacgctg accatgagat
     2701 tcgcgaacgg caccatcttg tcgaacttcg ggatcgccgg gatcgctcgc tctttcgcga
     2761 gctggcccgc ttggatgtgg tggccgccct tgaggcggcc gccggcgttg ccggcgagca
     2821 gcacgggcag gttcaagtga ttgtgcacgt tgccgtcgct catcccgctg ccgtagacga
     2881 acaacgagtt ctcgagcagg ttgctgtcgc cgtccggcgt gtccttcaag cgctgcaaga
     2941 atttggcgaa caggctcacg ttgtacgtgt cgatcttcgc cttcttctcc atctgcttcg
     3001 gatcgtagtt gttgtgcgag accggatggt ggccgtcgga gatgccgatc tgcgggtagc
     3061 tcaaacccga cagctcgcgg cccatcatga acgacgtcac gcgcgtgagg tcgccctgga
     3121 acgcgagcgc ctggagctcg aacatcaggt tcacgtgctc ctcgaacgac tcgggaatgc
     3181 cgctcggcgt cggcggcgcc tcgagccggc gctcggaccg ctggtcctcg gcctgcacga
     3241 tgcggcgctc gacctcgcgc acgttcgcga gatactcgtc gagccgcgcg cggtcgcgcg
     3301 tgcccaactt ggcttcgagg ccgcgcacgt cggaggccac ggcgtcgagg atgctcgtgc
     3361 tctgctcgag ccgggcgcgg cgcgcttcgg gcgtcgtgcc gtcgccgccg aacatgcgct
     3421 cgaacacgac gcgcgggttc agctccatcg gcagcggctg cgtcggcgtg cgccacgaga
     3481 tcgtgtccat gtaggtgcag aggaagtcgg cggcacacgt gccgaagata ttcgagctgt
     3541 cctcgatcgc gagctccatc gacgggaacg tcgtgccctg gccgatcttc tgggcgatga
     3601 cctggtcgat cgacgtgttg agctcgatca agttgccgcg cggcgcgtgc gcgcatgccg
     3661 agaaaaatcc ggggcatgcc gagtgcgcgt tcgcgccttc cgaatagaga cccgtcacga
     3721 cgttgaagtg ctcgcgcagg ttctcgagcg gcttcaagat gacgggcagg tcgaagccgg
     3781 tgccgacctt cttcggcatc cagacgtcca tgatcgcgcc gtgcgggata tagatcgtgc
     3841 cgaagcgcag cgtcggcgcg gccgccgtct gcgcgagcgc cgtgcgcgcc ggcaccatcg
     3901 cgtcgaggag cggcagcgcg accgcggcgc cgacgccctt caggaccgtt cgccgcgaca
     3961 agtgcttctt gctgatgaac ataagcgtct ctccgaccgg gcacccgaaa tggggacaga
     4021 tctattttct acggaaaata gatctgtccc catttcaatc tagcgcaatc gtctcggcct
     4081 gtctctcgcc tgcaatccgc atttgaaacg tgtcgctcgt cacgattccg agcacgagcg
     4141 acgagaaccg gtagtcgtcc tcggccgcct tgcggacgac cgcgcgggcc gtcggcatgt
     4201 cggccgtcgt gagctggcgc ccgagcgcgt aggtcatgag cttctcggtc gccgtccgga
     4261 cgaaccgctc gggacggctc aagattgcgt tgcgcaagtc gacgggaccg ttgatcggcc
     4321 cgaaatcgcg cagcacgtgg atcgggctgc cgtagtccct gacgggctcg ccgttgtcct
     4381 cgtcgcgcca gcggccgatg ccgtcgaaac gctcgagcac gaggccgatg gggtccatga
     4441 gctgatggca gctcgcgcag acgggatcgg cgcgatgccg ctccatgcgc gcgcgcatcg
     4501 agagcttctc ggccgtgttc tcgggcagcg tcggcacgtt cggcggcggc tgcggcgccg
     4561 gcgtgccgag gatgttcgtg agcacgtagt tgccgcggct cacgggagag gtgcggttcg
     4621 ggtacgacga caacgtcagc acgctcgcgt gcccgagcaa gccgtaacgg cgctcgtcgg
     4681 tctgcgcgat gcggcggaac tggtcgccgt agatgttcgg aatgccgtaa tgcttcgcga
     4741 gccgctcgtt gacgtacgtg tagtccgcgg acaagagctc ggtcagcggc cggtcctcgc
     4801 gcacgatcgt ctcgaacaag agctccgtct cacgaatggc gctctgccgc aagttgtcgt
     4861 cgaagtccgg gaacacgtac atgtccggcg tcgacgcgcg caaattgcgc aagtacagcc
     4921 attggctcgc gaacgttttt gcgagcgtcg tcgcgcgctc gtcggcgagc atgcgctcga
     4981 cctgcgctcg aagctctttg gaatcgccga gcttacccgc gacggcggcg tcgatgagct
     5041 cgtcgtcggg caggctattc cagaggaaga acgacagccg cgacgcgagc tcgaggtcgc
     5101 cgatgcgata gatcgagccg ggcaccgcgt cgacggggtc ctgctccacg cggaacagga
     5161 attgcggcga gacgagaatg taggcgagtc cggtctgcac gccggcctcg aacgtgcgcc
     5221 ccttcgcgcg ctcggcgccg tagagctcga gcagccgttc gcgctcgttg tcggtgatcg
     5281 ggcggcgata cgcgcggcgc gcgagcgccg tcagtatctg ctccgcgcac gaggccgctt
     5341 ccgattcctg cttgggaaag cacgagaaga tgcgctcgcg gctcggcgag cggcccggcc
     5401 ctgcaacggt cagcggccct tcgatgacga tctgatcgag ctccggaatg ccgacgtcca
     5461 tgcgcgcgtc gtgcttctcg cgctcgaacg cctgcagcac gtcttggcgc gcggcggagc
     5521 tcttcttcgc gaacgcgacg ccgacgtcgt gctggcccgc cgacacgacg acgcgcgttt
     5581 cgaaacggcg gtcgatctcg tcggccgtct tgccggggaa catctgcgcc ggcacttcgt
     5641 ccgccggacc gccgaaacgc gtcaacgtga gctgcgcgcc gtcgaggctg acctcgaggt
     5701 cgtgcggagt ctccgtgccg cgcacgacgt cgacggtgtt gcgccagagc ttcggcctga
     5761 tcacgtactc gccgtcgacg ggaaagaagt ggctcacgag catgccgccg cgcgtgccga
     5821 gcggcagccc gtcgacgtga tcgcgctgcg agcggtcggg cggcacgcgg taggtcgcga
     5881 cggccggcgc gaggctgaga tcgcccatcg cggcgctcgt gatcttccac gccgccgaga
     5941 ggtagcggtc gagcagcgac ggatcggtac cgagcacgtc ggcgatattg tcgaaaccgt
     6001 atgcctcgct gtcgttcggt aggtaagcgc tcgcgtcgat gtcgagcgcg agcaggtcgc
     6061 gaatcgcgtt gccgtactcg gtgcggttca agcgatgcag cgatttcgtg cccggattcg
     6121 gatgctcggc ggccgcgcga tcgagctgct cctcgaggta cgcgacgagc tcgtcggttg
     6181 ccgcggcttc cggcaggggc tctccggccg gcggcatcat gcggccgcgc agcttcttga
     6241 cgacggtctc ccagacgtcg gcgtgcgcgc cgacgtcggt cagcgcgagc cgctcgagcg
     6301 tgagctcgcc cgttcgctcg gcgtcgttgt ggcaatcggt gcagtagcgg tcgacgaggt
     6361 tcttggctgc cgcggcgcgt tgctcgacgc tcggctggca acccaccgag gcgagagcga
     6421 gggcaacgat cggcaaggcg cgcataaccc caagaacgta cgggaaataa cctcccgaag
     6481 gtaggggcgg aggtcaagaa ctcatcagtt gccgggccgc gtgaactcga acgtcagctc
     6541 cgagcacggc tgcttctcga acgggacctc cgacaacagc accacgtcct ggcccttatg
     6601 gggcgccgcg agatagacgg gatcttcggc cgtgtactcg cgcttcagcg cgaacgtttt
     6661 gggatccagc gtgaagcgct cgacgacgtg cagctgtggg ctgttgcgcg tcggcggcac
     6721 gagcacgccc tcggcgaagc cgaccgtgtc gacgacgagc gtgtcgccgt cccagcggcc
     6781 gatcgaatgg ccggcgttcg tcggcgtgag attcgccggg tgctcggtca tgccgaggtg
     6841 gaagcggcgc gaggcgctga acaggccgta gtcgatgtcg atgacgttct cgccgtcgac
     6901 ggtcgtctgc gtgatgcggt tgatcggcca gtcgaacgtc cagtcgaaca cgatgctcgt
     6961 cggcttgcac gacaggcgcg gattgtcctc gggcgaccac atgcgaaacg ccttcgaggt
     7021 ttcctcgccc ttcgccgtgt actgcgggcg cgacggcgcg ggattgcgcg cgcggatttg
     7081 ctcgagcgtg atcttgcccg ccgcgaaatc ctcgcgcagg ctgcgcggca cgagcgagcc
     7141 gttgccgccg ctcggcggaa tcgtcagcac ggcctgctcg acggcccaat cgcccgagat
     7201 gttcggctcg ccggacggca ggcgcagcgg ccgcttcgac gtgtcgacgg gcgcgtgcgt
     7261 gaactggtcg ttgcggtcga tcttcgtcgt gccgccgatc gcgacgttct cgagatagca
     7321 cgagtgcggg tcgtcgcggt gcgggtgcgc atggatgtcg acgtgccggc ccggcacgaa
     7381 ctcgtcgatc ttccagcccg agcggcggat cagcgtggcc gcgcgcatct cgcagcgcat
     7441 ctcgagcggc ttgccgtcgg cgtcgacggt ctcgaaatag agatacgagt gcgggttgac
     7501 gagctcgagc ttcgtcagcg tgcccgagta gtccttgaat tcctgcatct ggaacagacc
     7561 gaagccgtga tgcgcgagcg ccggggccgt agccgcaagc gcgagtgcga gcgtggctac
     7621 gtgaaagggc tttgtgcgag tggccatggc cgaatccttc agctgtgcgt cttgccggtt
     7681 gtgtgtctat tttaaccgcc cgccgctgcc cgcgagcctc aagaaagccc gacctcaggt
     7741 agcatggccg cccatgacga cgatccgccg cgcgccgtgg gcgctggccg tggctgcggc
     7801 gctcttggcc caagccggtc ccggcagccg tgccgacgct cagagctcgg ccgactgcga
     7861 cagcttgtgc agcgccgccg ccgagctcgt cgagcgcttc gagctgcgtg agagcgccgc
     7921 gcccgtgcgc gagcgcgtgg ggtgggagcg gcccaagaaa attgtcgtcg ccgacgtgcc
     7981 gctcgcggat tacctgcgcc gtgccgtgcc cggcgtcgag atcgtcggcg tggccggcct
     8041 t
//
LOCUS       DASSNN010000447         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1826276, whole genome shotgun sequence.
ACCESSION   DASSNN010000447 DASSNN010000000
VERSION     DASSNN010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826276"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /locus_tag="VFL84_08395"
     CDS             <1..1995
                     /locus_tag="VFL84_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608680.1"
                     /translation="PPAEAVVSAAAREGGPSFYRRLADDNVAELAAMIRQAAAEPKST
                     ERELALAVLLKRYSELDALGAVRLAREAGVGGMALSSVYGAWARKAPGQALAALSTVE
                     RPEAAATVALALIEALGNDAAAVRRVGSVLAARKEDPIGSPVFGPVSAAGPSALSIVA
                     QRWADLDPRRAYAVARELDDERVRQALESAALRALARTAPAEAFAHFATLGNDARQVA
                     AMSGTLIELARADPERVLAAAADFPPDARRLGVQVAIQQLAERDLLAAVRYVEGMSPS
                     PEREQMLQVVARSYGKRDVAAALAWARAQPQRDNLVAMVIAGVAEQDANRAIDLALEL
                     TSPIERDRALQFVAMSAVSRDDATVEALANRLLALEGSQPQSSVGFNIASMWAQRSPD
                     RAMSWLLANGQNASPNIFMQVGQQLAMRDPKNAIAYTTQIPAGARESWARGVGQGYAR
                     SDPEGAIDWLVQFRGEDWYARAAVTVAMDVAQMNPAAAARLVDGLDSARGAVETQRLT
                     NVIASSWAGREPAAAAAWALDRPTEQERAMAVQGVINAWSGRDADGARQWTLTLPQGQ
                     MRDGALTTLLTASTLRSPAGLDARVLNGFASEAARQQAVMQVVQNLAYADAARARSIA
                     DAHLTDAAMRAQVERVLEAARNNPERARLGPAGPTQVLRQ"
     gene            2088..>2710
                     /locus_tag="VFL84_08400"
     CDS             2088..>2710
                     /locus_tag="VFL84_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7608681.1"
                     /translation="MPLPFGQPPASLCIVRLSAIGDTCHTVPVVRAIQDAWPSTKLTW
                     IVGKTEHSLLAGLEGVELVVLNKGLGLRGYAAVHRALRGRKFDALLHMHASTRANIVS
                     TLVRAPLRIGFDRARARDQQWLFTNQKLPARPQRHVMDGLFEFAELIGVPRPATPRWD
                     IPVGPADLAAIAPLFKKGSRTLVISPCTGQRFRNYRNWRSDWYAQIA"
BASE COUNT          411 a          963 c          948 g          388 t
ORIGIN      
        1 ccgccggccg aggccgtcgt ctcggccgcc gcgagagaag gtgggccgag cttctatcgc
       61 cgcctcgccg acgacaacgt cgccgagctc gccgcgatga tcaggcaagc cgccgcggag
      121 ccgaagtcga cggagcgcga gctcgcgctc gcggtgttgc tgaagcgtta ttcggagctc
      181 gatgcgctcg gcgccgtgcg gctcgcgcgc gaagcgggcg tcggcggaat ggctttgagc
      241 tcggtctacg gcgcctgggc gcgcaaagct ccgggtcaag cgctcgcggc gctcagcacc
      301 gttgagaggc ccgaggccgc ggcgaccgtc gcgctcgcgt tgatcgaggc gctcggcaac
      361 gacgccgcgg cggtccgtcg cgtcggcagc gtgctcgcgg cacgcaaaga agacccgatc
      421 ggctcgccgg tgttcggccc ggtctcggcg gccgggcctt ccgcgctttc gatcgtggcg
      481 cagcgctggg ccgacctcga cccgcggcgg gcgtatgcgg tggcgcgcga gctcgacgac
      541 gagcgcgtgc ggcaagcgct cgagtcggcg gcgctacgcg cgctcgcgcg caccgcgcca
      601 gccgaggcgt tcgcgcattt cgcgacgctc ggcaacgacg ctcggcaagt cgccgcaatg
      661 agcggcaccc tgatcgagct cgcgcgcgca gatccggagc gcgtgctcgc cgcggcggcc
      721 gacttccccc ctgacgcgcg ccgcctcggc gtccaagtcg caattcagca gctcgcggaa
      781 cgcgatttgc ttgccgcggt ccgctacgtc gaaggaatgt cgccgagccc cgaacgcgag
      841 cagatgctgc aggtcgtcgc ccgcagctac ggcaagcgcg acgtcgccgc ggcgctcgcc
      901 tgggcgcgcg cccaaccgca acgagacaac ctggtggcaa tggtgatcgc cggagtcgcg
      961 gagcaagatg cgaatcgcgc gatcgatctc gcgctcgagc tcacttcgcc gatcgagcgc
     1021 gaccgggcgc tccagttcgt cgcgatgtcc gccgtcagtc gagacgacgc caccgtcgaa
     1081 gcgctcgcca atcgcctcct cgctctcgag ggctcgcagc cgcaaagctc ggtggggttc
     1141 aacatcgcgt cgatgtgggc acagcgctcg cccgatcgag cgatgagctg gctgctcgcg
     1201 aacggccaga acgcgtcgcc gaatatcttc atgcaggtgg gtcaacagct cgcgatgcgc
     1261 gatcccaaga acgcgatcgc ttacacgacg cagattcccg ccggcgcgcg cgagtcgtgg
     1321 gcgcgcggcg tcgggcaggg ttatgcacgg agcgaccccg aaggcgcgat cgactggctc
     1381 gtgcaattcc gcggcgaaga ctggtacgcg cgcgcggcgg tcaccgtcgc catggacgtc
     1441 gcgcaaatga accccgcggc cgccgcgcgc ctcgtcgacg gcctcgattc cgcacgcggc
     1501 gcggtggaga cgcagcggct aacgaacgtc atcgcctcga gttgggccgg ccgcgagccc
     1561 gcggcggcgg ccgcgtgggc gctcgatcgc ccgaccgagc aagagcgcgc gatggccgtt
     1621 cagggcgtga tcaatgcatg gtcggggcgc gacgccgacg gcgctaggca gtggacgctg
     1681 acattgccgc aaggccagat gcgagacggc gcgctcacga cgctgctgac ggcttcgact
     1741 ttgcggtcgc cggccgggct cgacgcgcgc gtgctcaacg gcttcgcatc ggaggcggcg
     1801 cgacagcaag cggtcatgca ggtcgtgcaa aacctcgctt atgccgacgc ggcgcgagcg
     1861 cgatccatcg ccgatgcgca cctgacggat gcggcgatgc gcgcgcaggt ggagcgcgtg
     1921 ctcgaggcgg cgcgcaacaa cccggagcgg gcccgtttgg gccccgccgg accgacgcag
     1981 gtgcttcgcc aataacggcg ccgctcgtta ccgaggcgca cgagcccggc gcggcgcatc
     2041 gtgtaccatg gcgctccttc gaatcagaac gtgccaataa cttcggcatg ccgctgcctt
     2101 tcggccaacc gcccgcgtcg ctctgcatcg tccggctctc cgcgatcggc gacacgtgcc
     2161 acacggttcc cgtcgtgcgc gcgatccagg acgcatggcc gagcacgaag ctcacgtgga
     2221 tcgtcggcaa gaccgagcat tcgctgctcg cggggctcga gggcgtcgag ctcgtcgtgc
     2281 tcaacaaagg gctcggctta cgcggctacg ccgccgtgca ccgcgcgctg cgtggccgca
     2341 agttcgacgc gctgctgcac atgcacgcgt cgacgcgcgc gaacatcgtc agcacgctcg
     2401 tgcgcgcccc cttacgcatc ggcttcgacc gcgcgcgcgc gcgcgaccag cagtggctgt
     2461 tcacgaatca gaagctcccg gctcggccgc agcgccacgt catggacggg ctattcgagt
     2521 tcgccgagct catcggcgtg ccgcgacccg cgacgccgcg ctgggatatc ccggttggcc
     2581 cagctgatct cgccgcgatc gcgccgctgt tcaagaaagg ctcgcgcacg ctcgtgatca
     2641 gcccctgcac gggccagcgg tttcgtaatt accgtaactg gcgctccgac tggtacgcgc
     2701 aaatcgccga
//
LOCUS       DASSNN010000448         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1308542, whole genome shotgun sequence.
ACCESSION   DASSNN010000448 DASSNN010000000
VERSION     DASSNN010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308542"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFL84_08405"
     CDS             <1..231
                     /locus_tag="VFL84_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608682.1"
                     /translation="ISRTVAALQNQSLVRCVANRSDGRSVLVSSTAKGRATLQKGMVR
                     TLEQIAELLTRLDAQELEALANIIRQARDSKA"
     gene            complement(244..1125)
                     /locus_tag="VFL84_08410"
     CDS             complement(244..1125)
                     /locus_tag="VFL84_08410"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7608683.1"
                     /translation="MKVGFVGLGRMGQGMARRLLDAGHDLTVFDQFAAQAASLAAAGA
                     HAAASVAEVAERSEIVVTMLVEDATVANVGLGPRGLSESLRKGAIHLVMGTHGVAVVR
                     ELEARHREAGQTLVAAPVLGRPDLAATGQLGIVVGGPEEAVARCTALLKSMGRRIFAA
                     GAKPESATAIKLANNAVLGCAMVAMAEGFSLVRKYDVTPQVFQDVMTEGLFASVAYKV
                     YGQKMVDESYDQVGSPIHVGLKDANLIAAAADLARVPMPSHNVYRDRLLGAVAHGDGD
                     RDQAVLAREQARASGLE"
     gene            complement(1232..1402)
                     /locus_tag="VFL84_08415"
     CDS             complement(1232..1402)
                     /locus_tag="VFL84_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608684.1"
                     /translation="MKILLTALVILVGSSQFACRPLVAGGVGAAVGVAAERERQEDKK
                     EDQERARNSRNR"
     gene            1531..2127
                     /locus_tag="VFL84_08420"
     CDS             1531..2127
                     /locus_tag="VFL84_08420"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HET7608685.1"
                     /translation="MRAFTNFALAALAAVTLVGVSAQNRDRDGDEDQLAASGLAERRQ
                     EILDMAKAALNELRKDRDAAALLDDAYGTAVFDTTKGGFVVTGAGGTGVAMRKDGSNP
                     VFMHMGSGGVGLGAGLENYKFIILFENEDVYKRFIDGEWSAGATAQAAAGRDGAAVVG
                     KFNNGVAVYHITDKGLIAQADVSGVRFWPSDRLNPGVG"
     gene            complement(2218..>2558)
                     /locus_tag="VFL84_08425"
     CDS             complement(2218..>2558)
                     /locus_tag="VFL84_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET7608686.1"
                     /translation="MLLNGGELNGHRLLGPRTVEMMTANHTGDMVNGQFGRPARGMGF
                     GLGMQVVLDPIAADLAVSKGAISWPGGSGVAHWIEPAENLVSIYFIQGGQGGPARQEL
                     ENAVRQAIIE"
BASE COUNT          417 a          853 c          847 g          441 t
ORIGIN      
        1 atctcgcgca cggtcgccgc ccttcagaat cagtcattgg tccgctgcgt cgcaaatcgc
       61 agtgacggcc gctcggttct cgtctcgagc acggccaaag gccgggcgac gctgcaaaag
      121 ggcatggtgc gcacgctcga gcagattgcc gagctgctga cgcgcctcga tgcgcaagag
      181 ctcgaagctc ttgccaacat catccgccag gcacgcgact cgaaggctta ggccccgatc
      241 ttcttattcg agccctgacg cgcgcgcttg ctcgcgcgcg agaaccgctt gatctcggtc
      301 tccgtcgcca tgggcaacgg cgcccaggag ccggtcgcgg tagacgttgt ggctcggcat
      361 cggcacgcgc gcgagatcgg cggcagcggc gatcaagttc gcatccttga gcccgacgtg
      421 gatcggcgag ccgacctggt catagctttc gtcgaccatc ttctggccgt acaccttgta
      481 cgccacgctt gcgaacaggc cttccgtcat gacgtcctgg aacacttgcg gcgtcacgtc
      541 gtacttgcgg acgagcgaga agccttcggc catcgcgacc atcgcgcaac cgagcacagc
      601 gttgttcgcg agcttgatcg ccgtggccga ttcaggcttc gcgccggccg cgaaaatgcg
      661 ccggcccatc gacttgagca gcgcggtgca gcgcgcgacc gcctcttcgg ggccgccgac
      721 gacgatcccg agttgccccg tcgccgcgag atccggccgc cccagcacgg gcgccgcgac
      781 gagcgtttgg cccgcctcgc gatgccgcgc ttcgagctcg cgcacgaccg cgacgccgtg
      841 cgtgcccatg acgagatgga tcgcgccttt gcgcagcgac tcgctcaagc cccgcgggcc
      901 gaggccgacg ttggcgacgg tggcgtcctc gaccagcatc gtgacgacga tttcgctgcg
      961 ctcggccact tcggcgaccg aggccgcggc atgcgcgccg gccgcggcga gcgacgccgc
     1021 ctgcgccgcg aattgatcga acaccgtgag gtcgtggccg gcgtcgagca ggcggcgtgc
     1081 catcccttgt cccatgcggc cgaggccgac gaaccctact ttcatgtgct cctcgaacgg
     1141 gcttaccgaa agaggggcga ctttgacgcg cgggctcgcg cgttgcaagc agcggcggcc
     1201 tcggcgcgca cgctgcgcgc cgtgcgcgca actagcgatt gcggctgttg cgcgctcgct
     1261 cttggtcttc cttcttgtct tcctgtcgct cgcgctcggc ggccacaccg accgcagcgc
     1321 cgacgccgcc cgcaacgagc ggtctgcacg cgaactgact ggagcccacg agtattacta
     1381 aggcggtcag caagattttc atctcagttt ctccgaattg cctttggccg ccatggggtt
     1441 gcagcaaccg tgccagcccg cgcggcgcgg ccgcgtggca tggaacttgc tctatccggc
     1501 ccagttcatg ggcttttgga ggaacgacag atgagagcgt ttacaaattt cgcactcgct
     1561 gcgttggcgg cggtaacgct cgtcggggtg agcgcgcaaa accgtgatcg cgacggcgac
     1621 gaggatcagc tcgccgcgag cggtcttgcg gagcgccgcc aagagatcct cgacatggcg
     1681 aaggccgcgc tgaacgagct gcgcaaagat cgcgacgcgg cagcgttgct cgacgacgcg
     1741 tacggcacgg ccgtcttcga cacgacgaag ggcggcttcg tcgtcacggg cgccggcggc
     1801 acgggcgtcg cgatgcgcaa agacggcagc aacccggtgt tcatgcacat gggctcgggc
     1861 ggcgtcggtc tcggtgcagg actcgagaac tacaagttca tcatcctgtt cgagaacgag
     1921 gacgtctata agcgcttcat agacggcgag tggagcgccg gagcgaccgc gcaagcggcg
     1981 gcgggccgtg acggagctgc tgtcgtcggc aagttcaaca atggcgtcgc ggtttatcac
     2041 atcacggaca aaggcttgat cgctcaagcc gacgtctcgg gtgtgcgttt ctggccgagc
     2101 gaccgtttga accccggcgt cggctaaaga tcagcgttct ggtgagagtg tgagagagac
     2161 tgcgcgcacg gcgttcgcgc cgtgcgcgca gatttcttgt gcgcgcctaa ggccggttca
     2221 ttcgatgatg gcttgccgca cggcgttctc gagctcctgc cgcgcggggc cgccctgacc
     2281 gccctgaatg aaatagatcg atacgaggtt ctcggcgggc tcgatccagt gggccacgcc
     2341 gctgccgccg ggccaactga tcgcaccctt cgagaccgcg agatcggcgg caatcggatc
     2401 gagcacgact tgcatgccta gaccgaagcc catgccgcgc gccggccgcc cgaattggcc
     2461 gttgaccatg tcgccggtgt gattggccgt catcatctcg accgtgcgcg gcccgagcag
     2521 gcggtggccg ttgagctcgc cgccgttcag cagcatcg
//
LOCUS       DASSNN010000449         3270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1210249, whole genome shotgun sequence.
ACCESSION   DASSNN010000449 DASSNN010000000
VERSION     DASSNN010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210249"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1916
                     /gene="argS"
                     /locus_tag="VFL84_08430"
     CDS             171..1916
                     /gene="argS"
                     /locus_tag="VFL84_08430"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET7608687.1"
                     /translation="MTAVHDPRAVLGARLQAAVAKAFGAEHAGVDPMVRRSERADFQA
                     DLAMGMAKALKRAPRQVAEAVVAAADLADVCERVEIAGPGFINLTLKKTFIEQGLAEL
                     ASDERLGVPAARVKDRVIIDYSAPNVAKEMHVGHLRSTIIGDALARLLEFAGHEVIRQ
                     NHIGDWGTPFGMLIEHLLDVGEAGSQASIGELGDFYRAARVKFDGDPKFADRARERVV
                     LLQAGDARTLALWRQLVELSKQYFSSVYERLGVSLRNDDVQGESSYNAALADVAAELE
                     LKGLASINDGALCAFPPGFTNRDGEPLPLIVRKQDGGYGYGATDLAALRYRTQTLRGT
                     RLLYVVGSPQAQHLAMVFAIGAQAGWLAPPARAEHVAFGSILGADKKMFKTRAGDTVL
                     LTDLLDEAVERADAELAKREADRNAAERRALAAEIGIGAIKYADLANDRIKDYVFDWD
                     RMLAAEGRTGPYLQYAHARIQSIFRKGAEAGVARMKGSAIAVGEQAERKLALELLDFG
                     TAVVEAGETLRPHRLAGYLYDLATAFTGFFETCPVLKAPDDATRASRLALCELTARVL
                     ARGLGLLGIAAPERM"
     gene            complement(1945..2148)
                     /locus_tag="VFL84_08435"
     CDS             complement(1945..2148)
                     /locus_tag="VFL84_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608688.1"
                     /translation="MSSLFARKRNPNPEMLTLPAFCVPADDTANLLLKREAQLEWMRA
                     AGVEYILDGSVKRHRPIPEKRVA"
     gene            complement(2386..3186)
                     /locus_tag="VFL84_08440"
     CDS             complement(2386..3186)
                     /locus_tag="VFL84_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HET7608689.1"
                     /translation="MATPTYRIFAIKYAHVGRKAWGNFVDGDPHEDWDMPLDYYVWAV
                     VGDGRTIVVDTGFDAPMAKKRDRKIVHPVEQGLEAVGVRHADVKDVVITHMHYDHCGN
                     GALFPNARFHLQDTEMEYVTGRCMCHHQINHAFEADDVSRMVHRVFAGRVQFHDGTDE
                     LAPGITVHKIGGHTKGLQCVRVATERGPVVLASDATHFYAHIDSRRVFRVLYNQGDML
                     EGYNTLERLAGARERVIPGHDPLVLERYPSASAGTEGWAVRVDLPPRR"
BASE COUNT          524 a         1070 c         1132 g          544 t
ORIGIN      
        1 cccacaagcg gcatgagcct cgtcggcact agaccgcgcc ttgctcgacg ggttcaagcg
       61 ttccggcgtc aaaccgcagg tgccgcccgt atactacgcg gcctcgaatc catggaaccg
      121 gccgtgcccg ctcatatcta cccccatcga cgcttcgcga ggtcgcgccc atgacggccg
      181 tgcacgatcc gcgcgccgtg ctcggcgcgc ggctgcaagc ggccgtcgcc aaggccttcg
      241 gcgccgagca tgcgggcgtc gacccgatgg tgcgccgctc ggagcgcgcc gacttccagg
      301 ccgacctcgc gatgggcatg gccaaggcgc tgaagcgcgc gccgcggcag gtcgcggaag
      361 ccgtcgtcgc cgcggccgac ctcgccgacg tctgcgagcg cgtcgagatc gcgggccccg
      421 gcttcatcaa cctgacgctg aagaagacgt tcatcgagca gggcctcgcc gagcttgcta
      481 gcgacgaacg gctaggcgtt ccggccgcgc gcgtcaagga tcgcgtcatc atcgactact
      541 cggcgccgaa cgtcgcgaag gagatgcacg tcgggcattt gcgctcgacg atcatcggcg
      601 acgcgctcgc gcgcctgctc gaattcgccg ggcacgaggt gattcgtcag aaccacatcg
      661 gcgactgggg tacgccgttc ggcatgctga tcgagcactt gctcgacgtc ggcgaggccg
      721 gctcgcaagc gagcattggg gagctcggcg atttctatcg cgccgcgcgc gtgaagttcg
      781 acggcgatcc gaagttcgcg gaccgtgcgc gcgagcgcgt cgtgctgctg caagccggcg
      841 acgcgcgcac gctcgcgctt tggcggcagc tcgtcgagct gtcgaaacag tatttctcga
      901 gcgtttacga acgcctcggc gtgagcttgc gcaacgacga tgtgcaaggc gagagctcgt
      961 acaacgccgc gctggccgac gtcgccgcgg agctcgagct taaagggctc gcctcgatca
     1021 acgacggggc gctgtgcgcg ttcccccccg ggttcacgaa ccgcgacggc gagccgctgc
     1081 cgttgatcgt gcgcaagcag gacggcggtt acggctacgg cgcgactgat ctcgcggcgc
     1141 tgcgctatcg cacgcaaacg ctgcgcggca cgcgcttgct gtacgtcgtc ggctcgccgc
     1201 aagcccaaca tctcgcgatg gtgtttgcga tcggcgcgca ggccggttgg ctggcgccgc
     1261 cggcgcgcgc cgagcacgtc gcgtttggct cgatcctcgg cgcggacaag aagatgttca
     1321 agacgcgcgc cggcgacacg gtcttgctca cggacttgct agacgaagcc gtcgagcgcg
     1381 cggacgccga gctcgcaaag cgcgaggccg accgcaacgc cgccgaacgg cgtgcgctgg
     1441 cggccgagat tggcatcggc gcgatcaagt acgccgatct cgcgaacgat cgcatcaagg
     1501 actacgtgtt cgattgggac cgcatgctcg cggccgaagg gcgcacgggg ccgtatctgc
     1561 aatacgcaca tgcgcgcatc cagtcgatct ttcgcaaagg cgccgaggca ggcgtggcgc
     1621 gcatgaaggg ttctgcgatc gcggtcggcg agcaagcgga gcgcaagctc gcgctcgagc
     1681 tcctcgactt cggcacggcg gtggtcgaag cgggcgagac gttgaggccg caccggttgg
     1741 ccggctatct ctacgatctt gcgacggcgt tcacggggtt cttcgagacg tgccccgtgc
     1801 tcaaagcgcc ggacgacgcg acgcgcgcct cgcgcctcgc gctctgcgag ctcacggcgc
     1861 gcgtccttgc gcgcggattg gggctgctcg gaatcgccgc gcccgagcgc atgtagcgcg
     1921 tcgggcgggt ctgctctacg cgtcctaggc gacgcgcttc tcggggatcg gccggtgccg
     1981 tttgaccgag ccatcgagga tgtactcgac gcccgcggca cgcatccatt cgagttgcgc
     2041 ttcgcgcttc agaagcagat tggcggtatc gtcggccggg acgcagaagg ccggcaaggt
     2101 cagcatttcg gggttcggat tccgcttccg tgcgaacaac gagctcatgt tcttttcctt
     2161 atcctcgcgg actaaaactt caggacgagt gaaattttcg acgcgcacgc atgcagcgtc
     2221 tactcgccgg gcgtggggct aggaataatg cggcggcgtg cgccggcggg ccgaattatg
     2281 taacggaact agcgcacgta aagagatccg gcactcgctg cgagccgcct ggcgcctgaa
     2341 gatcgaacat aacgcgattg acccgccgcc ctgcggcggc cggttttacc ggcgcggcgg
     2401 taagtcgacg cggaccgccc agccttccgt gccggcggag gccgaggggt agcgctcgag
     2461 gacgagcggg tcgtggcccg ggatgacgcg ctcgcgggct ccggccagcc gctcgagcgt
     2521 gttgtagccc tcgagcatgt cgccctggtt gtagagcacg cggaacacgc gccgcgagtc
     2581 gatgtgggcg tagaagtggg ttgcgtccga ggcgagcacg acggggccgc gctccgtcgc
     2641 cacgcgcacg cactgcaggc ccttcgtatg gccgccgatc ttgtgcaccg tgatgcccgg
     2701 cgcgagctcg tcggtgccgt cgtgaaattg cacgcggccc gcgaacacgc gatgcaccat
     2761 gcgcgagacg tcgtcggctt cgaacgcgtg gttgatctgg tgatggcaca tgcagcggcc
     2821 ggtcacgtac tccatctccg tgtcttgcaa atggaaacgc gcgttcggga acagcgcgcc
     2881 gttgccgcag tggtcgtagt gcatgtgcgt gatgacgacg tccttgacgt cggcgtgccg
     2941 gacgccgacc gcctcgagcc cctgctcgac agggtgcacg atcttgcggt cgcgtttctt
     3001 cgccatcggc gcatcgaacc cggtgtcgac gacgatcgtg cggccgtcgc cgacgacggc
     3061 ccagacgtag tagtcgagcg gcatgtccca gtcctcgtgc gggtcgccgt cgacgaaatt
     3121 tccccaggcc ttgcggccga cgtgcgcgta cttgatcgcg aaaattcgat aggtcggcgt
     3181 tgccatgatg agtgaccgaa gaagacgttg ccgcgattgt actggcgcgg ccgaggaggg
     3241 agtaagcttc agcgcctcct cgggagaccg
//
LOCUS       DASSNN010000450         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1690802, whole genome shotgun sequence.
ACCESSION   DASSNN010000450 DASSNN010000000
VERSION     DASSNN010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690802"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /gene="lon"
                     /locus_tag="VFL84_08445"
     CDS             <1..1398
                     /gene="lon"
                     /locus_tag="VFL84_08445"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET7608690.1"
                     /translation="QQRVKALKGPILCLVGPPGVGKTSLGQSIARATNRRFVRMSLGG
                     VRDEAEIRGHRRTYIGSMPGKIVQNLGKVKVRNPLFLLDEVDKMSMDFRGDPSSALLE
                     VLDPEQNATFNDHYLEVDFDLSEVMFVCTANSLNIPAPLLDRMEVIRLPGYTEDEKLN
                     IARRYLVPKQMRQNGLKDEELKVSDSAIIDIVRYYTREAGVRNLEREIANICRKVVKQ
                     LLLRPRKNTVKVDAENLPKLIGVRKFRYGRAEENDQVGQVTGLAWTEVGGELLTIEAA
                     IVPGKGKLIHTGQLGDVMQESIQAAMTVVRSRAAVLGLDEGFHVKSDVHIHVPEGATP
                     KDGPSAGIGMCAALVSALTKIPARSSVAMTGEITLRGEVLPIGGLKEKLLAAHRGGIE
                     TVLIPADNEKDLAEIPKNIKDKLNIVPVKWIDEVIELALQQTPRPIRTDVVVASPERD
                     TTVDDEDASEAALPH"
     gene            1559..1831
                     /locus_tag="VFL84_08450"
     CDS             1559..1831
                     /locus_tag="VFL84_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET7608691.1"
                     /translation="MNKAELVDAVAAAANLSKADAGRAVDAVVNSITAALKKGQQVSV
                     VGFGTFGVKHRAARSGRNPRTGETISIAASNVPGFKAGKALKDAVN"
     gene            1846..1921
                     /locus_tag="VFL84_08455"
     tRNA            1846..1921
                     /locus_tag="VFL84_08455"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1879..1881,aa:Val,seq:tac)
     gene            1932..2008
                     /locus_tag="VFL84_08460"
     tRNA            1932..2008
                     /locus_tag="VFL84_08460"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1966..1968,aa:Asp,seq:gtc)
BASE COUNT          439 a          642 c          675 g          359 t
ORIGIN      
        1 cagcagcgcg tgaaggcgct caagggtccg atcctgtgtc tcgtcgggcc cccgggcgtc
       61 ggcaagacgt cgctcggtca gagcatcgcg cgcgcgacga accgcaggtt cgtgcgcatg
      121 tcgctcggcg gcgtgcgcga cgaggccgag attcgcggcc atcgccgcac gtacatcggc
      181 tcgatgcccg gcaagatcgt gcagaaccta ggcaaggtga aggtgcggaa tcctctgttc
      241 ttgctcgacg aggtcgacaa gatgtcgatg gatttccgcg gcgatccgtc gtcggcgctg
      301 ctcgaggtgc tcgacccgga gcagaacgcg acgttcaacg atcactatct cgaggtcgac
      361 ttcgatctct cggaagtcat gttcgtctgc acggcgaaca gcttgaacat tcccgcgccg
      421 ctgctcgatc gcatggaagt catccggctt cccggctaca ccgaggacga gaagctcaac
      481 atcgcgcgcc gctacctcgt gccgaagcag atgcgccaga acggcctcaa ggacgaggag
      541 ctcaaggttt cggattccgc gatcatcgac atcgtgcgct actacacgcg cgaagcgggc
      601 gtccgcaatc tcgagcgcga gatcgcgaac atctgccgca aggtcgtgaa gcagctactg
      661 ctgcggccgc gcaagaacac cgtcaaggtc gacgcggaga acctgccgaa gctgatcggc
      721 gtgcgtaaat tccgttacgg gcgcgccgag gagaacgacc aggtcggtca agtcaccggt
      781 ctcgcgtgga ccgaggtcgg cggcgagctc ttgacgatcg aagccgcgat cgtgcccggc
      841 aagggcaagc tgatccacac gggtcagctc ggcgacgtca tgcaggaatc gatccaagcc
      901 gcgatgacgg tcgttcgcag ccgcgcggcc gtgctcgggc tcgacgaagg cttccacgtg
      961 aaatccgacg tgcacatcca cgtccccgag ggcgcgacgc cgaaggacgg cccgtccgcc
     1021 ggcatcggca tgtgcgcggc gctcgtctcc gcgctcacga agatccccgc gcgctcgagc
     1081 gtcgcgatga cgggcgagat cacgctgcgc ggcgaagtgc tgccgatcgg cgggctcaag
     1141 gagaaattgc tggcagcgca ccgcggcggc atcgagaccg ttttgatacc cgccgacaac
     1201 gaaaaggacc tcgccgaaat accaaagaac atcaaagaca agttgaacat agttcccgtc
     1261 aagtggatcg acgaggtcat cgagctcgcg ttgcagcaaa cgccgcgacc gattcgcacc
     1321 gacgtcgtcg ttgcgtcgcc agaaagagac actaccgtcg acgacgagga cgcgagcgaa
     1381 gcggcgttgc ctcactaacg agcgctcgct cagttgacac gcgaaaatcg cgcggtataa
     1441 ggtggccgct gtttcgaggg gcggccactc aattcgagac aaatcttccg gggtagacgt
     1501 gcgttcccgc acgtctggtt aacaaaaact tttgcgcaca ctcgtgtaag ggattcgaat
     1561 gaacaaagca gaactcgtcg atgcggtagc ggctgcggcc aacctgtcca aggcggatgc
     1621 aggtcgtgca gttgacgcgg tggtgaacag catcacggcg gcgctgaaaa aaggacaaca
     1681 ggtttccgtt gtcggctttg gcacgttcgg tgtgaagcac cgtgccgcac gcagcggccg
     1741 caatccgcga acgggtgaga cgatcagtat tgctgcgagc aacgtacccg ggttcaaagc
     1801 tggcaaggct ctgaaggacg ccgtaaacta gcccgctctg tcccagggtg cttagctcag
     1861 ctgggagagc gtcgccctta caaggcgaag gtcgggggtt cgatcccctc agcacccacc
     1921 agaaaagagc cggagtggta gttcagctgg ttagaatacc ggcctgtcac gccgggggtc
     1981 gcgggttcga gtcccgtcca ctccgccaat attcgagaga agggcagatt tattttcggc
     2041 gcaaccgaag gtaggtctgt ctttctcgca tttggcccgc ccgtcgttcg ggggtcatac
     2101 taagggccgc actcc
//
LOCUS       DASSNN010000451         4011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1592744, whole genome shotgun sequence.
ACCESSION   DASSNN010000451 DASSNN010000000
VERSION     DASSNN010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592744"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VFL84_08465"
     CDS             <1..1108
                     /locus_tag="VFL84_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HET7608692.1"
                     /translation="AFWPITQFVMGENWLNLYFAALIGLGLTAVIVVITEYYTGTGFS
                     PVKTVANASTTGHATNIIAGLGISMKATALPVVAVCASIWGAYALGELYGIAIAATSM
                     LSMTGMIVALDAFGPITDNAGGIAEMSKLPKEVRHVTDALDAVGNTTKAVTKGYAIGS
                     AGLAALVLFADYTHSLTEAGLSGLTFDLANHEVIIGLFLGGLIPYLFGAMAMEAVGRA
                     AGSVVNEVRRQFREIKGIMEGTTRPDYSTAVDLLTKAAIKEMIIPSLLPLLVPVIVGL
                     VLGPAALGGLLVGTIVTGLFVAISMTTGGGAWDNAKKYIEDGHHGGKGSDAHKAAVTG
                     DTVGDPYKDTAGPAINPLIKIINIVALLSVPLLG"
     gene            1187..2218
                     /locus_tag="VFL84_08470"
     CDS             1187..2218
                     /locus_tag="VFL84_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HET7608693.1"
                     /translation="MFCSKCGARASPDARFCASCGRTLEMPAEPEPAMPDRGDVEYVE
                     AAIGPSNTDYYLRKFERFASGGGYASWNWPAFFVPFLWMLYRKMWLWALLYLFVTPIA
                     FGLLFGILLSVLPETTGAAVGWGLWLLFIFIVLPMYANALYFKTCRSRIAEAKRYRAE
                     RRRQLERLWNMGGTSHVAWVVALLLPVPFAGILAAISIPAYQDYTIRAQVVEGMNLAA
                     VAKAAVAETFLNNGVVPQDREDAGLTATGTETRGKYVSSVEIIDGRIDIFYGGEANRV
                     IAGQVLSITPYGIQRSGDTWSIAWRCGYAQVPDAATHEIAPYETGTIQPKYLPAACRP
                     QSYSAQQYQ"
     gene            2237..3100
                     /locus_tag="VFL84_08475"
     CDS             2237..3100
                     /locus_tag="VFL84_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7608694.1"
                     /translation="MRLSTLLMISFFGVGAASTASAETITADGPVVYGHHHFNTTDMA
                     AQKRFFVETLGGKLATIGTNNLEVIEFPNVFLFFRPMQAPTGGTIGSTVNHVGFSVPD
                     LKPVVAKLKANGFKMITSDSVAASVKVTDDIAAASPTTNIAFVLGPEDIKVELVEVKA
                     QAAPIQLHHVHFFGEQQAEMRAWYAKVFGAAVQPQGAGQVFVTAQLPGVTLSFSPSQT
                     PTVATQGRALDHIGFEIKNLEAFAKKLEADGIPLTVSYRRVDALNIAIAFIKDPWGTN
                     IELTEGLRAIN"
     gene            complement(3097..3945)
                     /locus_tag="VFL84_08480"
     CDS             complement(3097..3945)
                     /locus_tag="VFL84_08480"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7608695.1"
                     /translation="MALGACLCGTVRYELDGPFTMMMHCHCSMCRKHHGAPFATFVAA
                     AQSGFRWRSGEGAVGRYASSPQGSRSFCRQCGSVAPMLLPDAGIAVAPAGNLDGDLGI
                     RPQAHMFAGSKAAWYTIADALPQHAGVPPEFGGGEGVERPTVAQREGVADGSCLCGEV
                     AFEYRAPQRMYQCHCSRCRKARSAAHGANVFVKLEDFAFTRGEELVTQYKVPEAQRFT
                     TAFCTRCGGKAPSVQRARGVVVVPAGTLDTDPLMRPQAHIFVASKAPWFEITDAYPQF
                     AELPPP"
BASE COUNT          693 a         1336 c         1317 g          665 t
ORIGIN      
        1 cgcgttctgg cccatcacgc agttcgtcat gggcgagaac tggctcaacc tttatttcgc
       61 cgcgctgatc ggcctcggcc tcacggcggt catcgtcgtg atcaccgagt actacacggg
      121 cacgggcttc tccccggtca agaccgttgc gaacgcgtcg acgacgggtc acgcgacgaa
      181 catcatcgcg ggcctcggca tctcgatgaa ggcgacggcg ctgcccgtgg tcgccgtctg
      241 cgcgtcgatc tggggcgcgt acgcgctcgg cgagctctac ggcatcgcga tcgcggccac
      301 gagcatgctg tcgatgacgg gcatgatcgt cgcgctcgac gcgttcggcc cgatcaccga
      361 caacgccggc ggcatcgccg agatgtcaaa gctgccgaag gaagttcggc acgtgaccga
      421 cgcgctcgac gccgtcggca acacgacgaa ggccgtcacg aagggctacg cgatcggctc
      481 cgccggtctc gcggcgctcg tgctgttcgc cgactacacg cacagcctga ccgaggcggg
      541 tttgagcggc ctcacgttcg acctcgctaa ccacgaggtc atcatcggcc tgttcctcgg
      601 cggcttgatt ccttatcttt tcggcgcgat ggcgatggaa gccgtcggcc gcgccgcggg
      661 ctcggtcgtc aacgaggtgc gccgccagtt ccgcgagatc aaaggcatca tggaaggcac
      721 gacgcggccg gactactcga cggccgtcga cctgctgacg aaagccgcga tcaaggaaat
      781 gatcattccg tcgctgctgc cgctcttggt tcccgtgatc gtcggtctcg tgctgggccc
      841 agccgcgctc ggcggcctgc tcgtcggcac gatcgtgacg ggcttgttcg tcgcgatctc
      901 gatgacgaca ggcggcggcg cgtgggacaa cgcgaagaag tacatcgagg acggccacca
      961 cggcggcaag ggctccgatg ctcacaaagc cgccgtcacg ggcgacaccg tcggcgatcc
     1021 gtacaaggat acggcgggtc ccgcgatcaa cccgctgatc aagatcatca acatcgtcgc
     1081 gctgctcagc gtgccgctgt tgggctagga gcaacgctcg cgtagaacga cgaaggccgg
     1141 cagcaacact gccggccctt ttctttgagc agataggggg acgaccatgt tctgcagcaa
     1201 gtgcggtgcg cgagcgagcc cggacgcgcg gttttgcgca agctgcggac gcacgctcga
     1261 gatgccggcc gaacccgagc cggcgatgcc cgaccgcggc gacgtcgagt acgtagaagc
     1321 cgcgatcggc cccagcaaca ccgactacta cctgcgcaag ttcgagcgct tcgcctcggg
     1381 cggcggatac gcgtcgtgga actggccggc gttcttcgtg ccgttcctgt ggatgctgta
     1441 tcgcaagatg tggctttggg cgctgctcta cttgttcgtg acgccgatcg cgttcggcct
     1501 cctgttcggc atcctgttga gcgtgttgcc cgaaacgacg ggcgccgccg tcggctgggg
     1561 gctatggctc cttttcatat tcatcgtgct gccgatgtac gcgaacgcgc tgtacttcaa
     1621 gacgtgccgg tcacgcatcg cggaagcgaa acggtatcgc gccgagcgcc ggcggcagct
     1681 cgagcggctt tggaacatgg gcggcacgag ccacgtcgct tgggtcgtcg ccttgctgct
     1741 gccggtgccg ttcgccggca tcctcgcggc gatttcgatc ccggcctatc aggactacac
     1801 gatccgcgcg caggtcgtcg agggcatgaa cttggccgcc gtggccaagg ccgctgtcgc
     1861 cgagacgttc ctgaacaacg gcgtcgttcc gcaggatcgc gaagatgcgg gcctgacggc
     1921 gaccggcacg gagacgcgcg gcaagtacgt cagctccgtc gaaatcatcg acggccgcat
     1981 cgacatcttc tatggcggcg aggcaaaccg cgtcatcgcg ggccaggtgc tgtcgatcac
     2041 gccgtacggc attcagcgca gcggcgacac atggtccatc gcgtggcgct gcggctacgc
     2101 tcaggttccg gacgcagcga cgcacgagat cgcgccgtac gagacgggta ccatccagcc
     2161 gaagtacttg ccggccgctt gtcggccgca aagctactcg gcgcagcagt atcagtaaac
     2221 ccacgagaga gctccgatgc gactatccac gcttctcatg atctcgttct ttggcgtcgg
     2281 cgcagcttcg actgcttcgg cagagacgat cacggccgac gggcccgtcg tctacggcca
     2341 ccatcacttc aatacgaccg acatggccgc gcagaagcgg ttcttcgtag aaacgctcgg
     2401 cggcaagctc gcgacgatcg gcacgaacaa cttggaggtc atcgagtttc cgaacgtgtt
     2461 cctgttcttt aggccgatgc aggcgccgac gggcggcacg atcggcagca cggtcaacca
     2521 tgtcggcttc tcggtgccgg atttgaagcc cgtcgtcgcg aagctcaagg cgaacggctt
     2581 caagatgatc acgagcgact cggtcgcagc gtcggtcaag gtcacggacg acatcgccgc
     2641 ggcgagcccg acgacgaaca tcgcgttcgt gctcggcccc gaggacatca aagtcgagct
     2701 cgtcgaggtc aaggctcaag ccgcgccgat tcagctgcat cacgtccact ttttcggcga
     2761 gcagcaagcc gagatgcgcg cgtggtacgc gaaggtcttc ggcgcggccg tgcagccgca
     2821 aggcgcgggc caggtgttcg tgacggctca gctgcccggc gtcacgttga gcttctcgcc
     2881 gtcgcagacg ccgaccgtcg cgacgcaagg ccgcgcgctc gatcacatag gcttcgagat
     2941 caagaacctc gaggcgttcg cgaagaagct cgaagccgac gggattccgc tgaccgtgtc
     3001 gtaccgccgc gtcgacgcgt tgaacatcgc gatcgcgttc atcaaggacc cctggggcac
     3061 gaacatcgag ctgaccgagg gcctgcgcgc catcaactag ggcggcggca gctccgcgaa
     3121 ttgcgggtac gcgtcggtga tctcgaacca cggcgctttc gacgcgacga agatgtgcgc
     3181 ctgcggacgc atcagcgggt ccgtgtcgag cgtgccggcc ggcacgacga cgacgccgcg
     3241 cgcgcgctgc acggacggcg ctttaccgcc gcagcgcgtg cagaacgcgg tcgtgaaccg
     3301 ctgcgcctcg ggcactttgt actgggtcac gagctcctcg ccgcgcgtga acgcgaagtc
     3361 ctcgagcttc acgaacacgt tcgcgccgtg cgccgcgctg cgcgccttgc ggcagcgcga
     3421 gcagtggcac tgatacattc gctgcggggc gcgatactcg aaagccacct cgccgcagag
     3481 gcagctgccg tcggcgacgc cttcgcgttg cgcgacggtc ggccgctcca cgccttcgcc
     3541 gccgccgaac tcgggcggca cgccggcatg ctgcggcagc gcgtcggcga tcgtgtacca
     3601 cgcggccttc gagccggcga acatgtgcgc ttgcggtcga atgccgaggt cgccgtcgag
     3661 gttgcccgcc ggagcgaccg cgatgccggc gtcgggcaac agcatcggcg cgaccgaccc
     3721 gcattgacgg cagaacgatc ggctgccctg cggcgacgac gcgtatcgac cgacggcccc
     3781 ctcgccgctt cgccagcgga agcccgactg cgcagccgcg acgaacgtcg cgaacggcgc
     3841 gccatgatgc ttgcggcaca tcgagcagtg gcaatgcatc atcatcgtga acggcccgtc
     3901 gagctcgtag cgcacggtgc cgcaaagaca cgcccctaaa gccacgttcg gttcctccgt
     3961 ggacgactgc cgacagatgt taaccccaaa cggcgggcgc tcgagaaggc g
//
LOCUS       DASSNN010000452         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1308764, whole genome shotgun sequence.
ACCESSION   DASSNN010000452 DASSNN010000000
VERSION     DASSNN010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308764"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2054)
                     /locus_tag="VFL84_08485"
     CDS             complement(<1..>2054)
                     /locus_tag="VFL84_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7608696.1"
                     /translation="ERFAFELGLSPELEVASVEDCTAFFNEALDEGLTAAAVREMNAL
                     GDRLGVEDWRADVKRIADEARVNDIAPGALPEMGKRCADDLLAHFPAKLNGDLDAGLL
                     AAVHRALERIDLALDTTAGTAAYVEKLRSAIYALRRPPCAWKHWLTLAKEKATKKSEA
                     HADRVREAASVYAAHPRFQAEIRRYLERVYSLAADALERFQRIKMRRGLIDFNDMEQY
                     LLHALDEQAVRARLGEELELLLVDEFQDTSPMQLAIFMKLAALADKAVFVGDVKQAIY
                     AFRGCDPDLVFATLEALTARGGESDVLEHSWRSRPALVDYVNSVFGQAFAGRMRPEQI
                     ELRPKRAERTGEPAVLRWSIKGKDEVRAAALARAVGDLVASAFRVVDPDSDAPRPVRW
                     GDIAVLAASNDHVETLARALRAAQIPIRMTLKGLLEVPEVCLAKACLARLNDHTDTLA
                     TAEIVSMCDCSAPETWLGARLRWLAQDEDGYGWAETAGEAAGVAAHPVVAKLKALRES
                     LGAESPVEIVARVLNYVGIREVVTAWGPNAIKAAQRQRNLDALLNLAVEYERHCDSQH
                     LAATLTGFLFWLEHPHSAELDIQPAVTTGDAVHVLTYHKAKGLEWPVVVATDLHYRWR
                     PRLWSVRVEGPEDFDLERPLAGRVLRYWPQVFGQHEKDVPVLDVLRECEEGRRCLERG
                     VG"
BASE COUNT          330 a          728 c          675 g          321 t
ORIGIN      
        1 tcgcccacgc cccgctccag gcagcggcgg ccttcttcgc attcgcgcaa cacgtcgagc
       61 acgggaacat ctttctcgtg ctgcccgaag acctgcggcc aataccgcaa cacgcgcccg
      121 gcgagcggcc gctcgagatc gaagtcctcg ggtccctcca cgcgcacgct ccataagcgc
      181 ggccgccagc ggtagtgcag gtccgtggcc acgaccaccg gccactcgag acccttggcc
      241 ttgtgatagg tcagcacgtg cacggcgtcg cctgtcgtca cggcaggctg gatgtcgagc
      301 tccgcggaat gcgggtgctc tagccagaac aggaagccgg tcaaggtcgc ggcgagatgc
      361 tgcgagtcgc agtggcgctc gtactcgacc gcgagattca gcagcgcgtc gagattcctc
      421 tggcgctgcg cggccttgat cgcattcggc ccccaggccg tcactacctc gcggatgccc
      481 acgtaattga gcacgcgcgc cacgatctcg acgggcgact cggcgccgag actctctcgc
      541 agcgccttga gcttggccac gacgggatgc gccgccacgc cggccgcctc gcccgccgtc
      601 tccgcccatc cgtagccgtc ctcgtcctgc gccagccagc gcagcctcgc gccgagccag
      661 gtctccggcg cggagcaatc gcacatcgaa acgatctcgg ccgtcgccag cgtatccgtg
      721 tgatcgttga gccgcgcgag acacgccttc gcgagacaga cctcgggcac ctcgagcagg
      781 cccttgagcg tcatgcggat cggaatctgt gccgcgcgca gcgcccgcgc gagcgtttcg
      841 acatggtcgt tactcgccgc aagcaccgcg atgtcgcccc agcgcacagg acgcggcgcg
      901 tcggaatcgg gatcgacgac ccgaaaggcc gaagctacga gatccccgac ggcgcgcgcc
      961 agggccgcgg cgcgaacctc gtccttgccc ttgatcgacc agcgcagcac ggccggctcg
     1021 ccggttcgct cggcgcgctt cggacgcagc tcgatctgct cggggcgcat gcggcccgcg
     1081 aaggcttgcc cgaacacgct gttgacgtag tcgacgagcg cgggacgcga gcgccacgag
     1141 tgctcgagca cgtcggactc gccgcctcga gccgtgagcg cttcgagcgt cgcgaatacg
     1201 agatccggat cgcagccgcg gaaggcgtag atggcttgct tgacgtcgcc gacgaagacg
     1261 gctttgtcgg ccagcgcggc gagcttcatg aagatcgcga gctgcatcgg gctcgtgtcc
     1321 tgaaactcgt ccacgagcag cagctcgagc tcctccccga gccgcgctcg cacggcctgc
     1381 tcgtcgagcg cgtgcagcag atattgctcc atgtcgttga agtcgatgag cccgcgccgc
     1441 atcttgatgc gctgaaaacg ctcgagcgca tcggccgcga gcgagtagac ccgctcgaga
     1501 tagcggcgga tttcggcctg gaagcgcgga tgcgccgcat agaccgacgc cgcctcgcgg
     1561 acgcggtcgg cgtgcgcctc gcttttcttc gtcgctttct ccttggccaa ggtgagccag
     1621 tgcttccagg cgcaaggcgg acggcgcagc gcataaatcg cgctcctgag cttctccaca
     1681 tacgccgccg tgccggcggt cgtatcgagt gcgagatcga tgcgctcgag cgcgcgatgc
     1741 accgcagcga gaagcccggc atcgagatcg ccgttcagct tggcgggaaa gtgagcgagc
     1801 aaatcgtcgg cgcagcgctt gcccatctcg ggcagcgcgc cgggggcgat gtcgttgact
     1861 cgggcctcgt cggcgatgcg cttgacgtcg gcccgccaat cctcgacgcc gagccggtcg
     1921 ccgagagcgt tcatctcgcg cacggccgcg gcggtgagtc cctcgtccag cgcctcgttg
     1981 aagaacgccg tgcagtcctc gacgctcgcg acttcgagct cgggcgagag gccgagctcg
     2041 aacgcgaaac gctc
//
LOCUS       DASSNN010000453         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1211148, whole genome shotgun sequence.
ACCESSION   DASSNN010000453 DASSNN010000000
VERSION     DASSNN010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211148"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..539)
                     /locus_tag="VFL84_08490"
     CDS             complement(144..539)
                     /locus_tag="VFL84_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7608697.1"
                     /translation="MNGYVDDIEDATLRNTDFRRVLYTGSNLQLVLMTLQPGEEIGAE
                     THEDRDQFFRIVEGQGVVEIDGRRHAVEDDYAVVVPAGARHNVINSGRSPLKLYTLYG
                     PPEHRDAVVHRTKREAVGGDEHFDEAKTE"
     gene            812..2632
                     /locus_tag="VFL84_08495"
     CDS             812..2632
                     /locus_tag="VFL84_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608698.1"
                     /translation="MVLRYGATATRVALATGLASVLSFVAYGQFGGNNPPPYTPAADA
                     KDLKSVLFNWPWHLGMLRGIEEHELTVTLEYRGEGTIQVNGQPCAITPYKEAREGELG
                     TSGYRISNAYQFSGSRTQINCTLPNGRTYSNIEVVSGKYAWDEDRPGAGLVEGEGTAK
                     PMRESVDERLIRLWASPQGAPKAALAGAGIDPLKVFRNPGGLLQEGTSKIGDTSVTWD
                     GNTPVVTFPIPGVDGATAVARLDERFMASSVVVKNGRDTYEFTYSNYEDTNNPLNLIE
                     TKFPRKMVERKNGQVVRDLNTVVTETGSVYVVVPVPPSVGPTEPLPLFGSDQQAPALT
                     SNAPTPRLADGKPDLTGSWRSDGNKFFIWRYGNRRCAPTQLDGCTPAWNQTLDFEFEA
                     ASRFGPNRPLYRPEHWDTVIYNDMWTNRLDPIMTCQPMGQPRQGPPGRIFRTENDITL
                     LYGRGGDGGGGYSDFRVIATDGRAFDERQLLQTKYTGHSIGHWEGDTLVIESRGFTPE
                     TWLARGGFFHSDKMKVFERFTRKGDQLLYEVTVEDPDVLLQPWTLKPMLMTVAPPAPP
                     PQAGGPGGGGGGFGGASLIGVERGNCQEEELDDIAWQIRH"
BASE COUNT          497 a          887 c          879 g          473 t
ORIGIN      
        1 gcaacgagcg cggcctcgat cggatgcgcc gttttttccg acgtgatctc gacctctgcg
       61 agctcttcca acggcaacca gtcggcgttg gggggcgcga ttcgttcgga cggtccaatc
      121 actcgtttgc gcatgtcgtc gttctactcg gtcttggcct cgtcgaagtg ctcgtcgcct
      181 ccgaccgctt cgcgtttggt tcgatggacg acggcatcgc ggtgctccgg aggaccgtag
      241 agcgtataga gcttcaacgg cgaccggccg gaattgatca cgttgtgccg cgcgcccgcc
      301 ggaacgacta cggcgtagtc atcctcgacg gcatgacggc gtccgtcgat ctcgaccacg
      361 ccctgccctt ctacgatgcg gaaaaactga tcgcgatcct cgtgcgtttc cgcgccgatc
      421 tcctcgcccg gctgcagcgt catcagcacg agctgcaggt tggatcccgt atagagcacg
      481 cggcgaaagt ccgtgttgcg cagtgtcgcg tcttcaatgt cgtcgacgta tccgttcatg
      541 gagcgtccct cggtttgcaa cggcagctgg ccgctgctgg tggaagtcga atcgtgcaag
      601 agacatgccg cgccccgcct caccgcttac cgcgccccag gacaatcacc ggcctaccat
      661 cgaatcggag cgccgggcac gcgatagggc gtcatgactg cggcgggccc tccatcactt
      721 tgcttttctc tgcattcgcc ggccggcgca attgggacgg atgggcgcgg cagtgatagt
      781 ctcagcagca tcttggtata gggggagcac catggtcttg agatatgggg cgaccgcaac
      841 gcgtgtcgcg ctcgcaaccg gcctcgcgtc ggtactgtcg ttcgtcgcgt acggccagtt
      901 cggcggcaac aacccgccgc cctatacgcc tgcggcagac gctaaagacc tgaagtccgt
      961 gctgttcaac tggccgtggc acttgggaat gctgcgcggc atcgaggagc acgagctcac
     1021 ggtcacgctc gagtatcgcg gcgaaggcac gatccaggtc aacggtcagc cctgtgcgat
     1081 cacgccctac aaggaggcgc gcgagggcga gctcggcacg tcgggctacc gcatcagcaa
     1141 cgcgtaccaa ttttcgggca gccgcacgca gatcaattgc acgctgccga acggccgcac
     1201 gtattcgaac atcgaggtcg tcagcgggaa gtacgcctgg gacgaagacc ggcccggcgc
     1261 cggcctcgtc gagggcgaag gcacggccaa gccgatgcgc gaaagcgtcg acgagcgctt
     1321 gatccggctc tgggcgagcc cgcagggcgc accgaaagcg gccttggcgg gcgccggcat
     1381 cgaccccttg aaagtgttcc gcaaccccgg cggtctgcta caagaaggca cgtcgaagat
     1441 cggcgacacg tcggtcacgt gggacgggaa cacccccgtc gtgacgttcc cgattcccgg
     1501 cgtcgacggc gcgacggccg tcgcccggct cgacgagcgc ttcatggcct cgtccgtcgt
     1561 cgtcaagaac gggcgcgata cgtacgagtt cacgtacagc aactacgagg acacgaacaa
     1621 tccgctgaac ctgatcgaaa ccaagtttcc gcgcaaaatg gtcgagcgaa agaacggcca
     1681 ggtcgtgcgc gacttgaaca cggtcgtgac ggagacgggc agcgtctacg tcgtcgtgcc
     1741 ggtgccgccg agcgtgggcc ccacggagcc gctgccgctc tttggcagcg accagcaagc
     1801 gccggcgttg acttccaacg cgccgacgcc gcgtttggcc gacggcaagc ccgacctcac
     1861 gggcagctgg cgaagcgacg gcaacaagtt cttcatctgg cgctacggca accgccgctg
     1921 cgcgccgacg cagctcgacg gctgcacgcc ggcgtggaat cagacgctcg acttcgagtt
     1981 cgaggcggct tcgcgcttcg gcccgaatcg cccgctgtat agacccgagc actgggacac
     2041 ggtcatctac aacgacatgt ggacgaaccg gctcgatccg atcatgacct gtcaaccgat
     2101 gggtcaaccg aggcagggac cgcccgggcg catcttccgc acggaaaacg acatcacgtt
     2161 gctctatggt cgcggcggcg acggcggcgg cggttattcg gacttccgcg tgatcgcgac
     2221 cgacggccga gcgttcgacg agaggcagct gctccagacc aagtacacgg gtcattccat
     2281 cggccattgg gaaggcgaca cgctcgtgat cgagtcgcgc ggctttacgc ccgagacctg
     2341 gctcgcgcgc ggcggcttct ttcactcgga caagatgaaa gtcttcgaac gcttcacgcg
     2401 caagggcgat cagttgctct acgaagtgac ggtcgaggat ccggacgtgt tgctgcagcc
     2461 atggacgctg aagcccatgc tgatgaccgt tgctcctccc gctcctccgc cacaagcggg
     2521 tggcccgggc ggcggcggcg gcggcttcgg tggcgccagc ttgattggcg tggagcgcgg
     2581 caactgccaa gaagaagagc tcgacgacat cgcctggcag attcgccact gagaacgtgc
     2641 caaccaaagc gccggacgtc ttcgagacgt ccggcgtttt tttgctcagc gccagccgag
     2701 ctcgggtgcg acgtacttga gaatgctctc gatcgc
//
LOCUS       DASSNN010000454         3444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_457552, whole genome shotgun sequence.
ACCESSION   DASSNN010000454 DASSNN010000000
VERSION     DASSNN010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457552"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFL84_08500"
     CDS             complement(<1..333)
                     /locus_tag="VFL84_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET7608699.1"
                     /translation="MDIKTSAAPLSRPRLIIDERAYPRLLALAERARVQAPELADRLI
                     EEIERAELRPTSEMPEDVVTLGSEVTFRHGDRMQTVQIVAPEDADIEQKRISVLTPVG
                     AALLGLAVG"
     gene            complement(553..771)
                     /locus_tag="VFL84_08505"
     CDS             complement(553..771)
                     /locus_tag="VFL84_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608700.1"
                     /translation="MKAIEDLLPDVRDGLTREERIVLYVLHETQKERGGRDVPTAMLW
                     GRVCEHFYISPEDLSDMLARLGARKDSI"
     gene            complement(776..3079)
                     /locus_tag="VFL84_08510"
     CDS             complement(776..3079)
                     /locus_tag="VFL84_08510"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HET7608701.1"
                     /translation="MNRSKQGICAALACAAAGSAAAQNNAVLEDPGQKSVRVVRTATP
                     PVIDGDLSDAVWANAAVVDDLHQVTPIEYAEPDERTEILILYDDDALYVAARLYDDPN
                     GITARNMRQNDNLGQDDRFYVTIDPFNDRRSGYFFGLNPNGVRADGLYRNVTEFYGDW
                     DSIYDAAAGRFEGGWTAEMEIPYKSISFDPATDTWGLNFSRTVVRKNEIIAWVSRNRA
                     YNPAVSGLAIGFEGLQQGIGLEVVPSLAVSDRKDFMAGGTDSETKPSLDLAYRITPQL
                     NGSLTINTDFSVTEVDDRQVNLTRLGLFFPEKRDFFLREADIFEFGGIGGQRQSSIPG
                     LNTLAQNGRPFFSRRIGLSTTGQVVDLEYGGKVSGRVGRWEVGGLSVRQDAHSVTVLR
                     GREPIGVECAALPTVPATSSCVIPADTLSVARAKLGVLEESNVGVITTYGSPTSGAEN
                     ALAGLDFQYRNSRLPGSRALEGDVFFQQSDTDGFDGDQDSYGASLRFPSSQGVRGILQ
                     YRRYGAQFNPALGFLDSAGISDTFFNVGYMLRPSGGNLESWLANFDYQRIDYLNGGGL
                     KTEAYFLRPLTFTNRTNDTLTVAFSDFTERLIEPFQIYPGVFVPVGTYDADSRGIQGG
                     TGGHRKLAAFMRVVEYPDGKFYGGSRSDRYLELTWRPSAFFRGSLSYDYSDVDLPVPN
                     GAFEVRLLRAGFDFAFSPTISWVNLIQYDNVSEVGGINMRLHWIPEAGREVFFVINHN
                     VEDLDHDDEFHSAFSDATAKVSYTFRF"
     gene            complement(3167..>3444)
                     /locus_tag="VFL84_08515"
     CDS             complement(3167..>3444)
                     /locus_tag="VFL84_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608702.1"
                     /translation="IKYVLVEPACASGHLAPLHIAALIGVLLALAFGALAFHEWRAAG
                     GGWPDHGGGSAGRTRFMGVLAMLMAGSAALVMIAQWIPDWIVSPCQH"
BASE COUNT          584 a         1134 c         1142 g          584 t
ORIGIN      
        1 cccgaccgcg agcccgagca gcgcggcgcc gacgggcgtc agcaccgaga tgcgcttctg
       61 ctcgatgtcg gcgtcctcgg gggcgacgat ctgcacggtc tgcattcggt cgccgtgccg
      121 aaacgtcact tccgatccca acgtgacgac gtcctcgggc atctcgctcg tcgggcgaag
      181 ctcggcccgc tcgatctcct cgatgagccg gtcggctagc tcgggggcct ggactcgcgc
      241 gcgctcggcc agcgcgagca ggcgcggata ggcgcgctcg tcgatgatga gccgcggtcg
      301 ggacagcggc gcagccgatg tcttgatgtc catgtcgttc tccgtcgtac gaaacaaatg
      361 ctcgaagctc ggccgaggtg gcgagcttcg acggtcaggt cttggaagtc gtgcctgggc
      421 gcgccgggcg ctcgtcgcgc cgggcgatcc aggcttagca gccttggatc agcttgtcgg
      481 cgcgacgcgg gccgagacga aatgcagctg atgtaaacat gacaacgatg gtcgccaatc
      541 ggcgcgccgg agtcaaatcg aatctttccg cgcaccgagc cgcgcgagca tgtccgacaa
      601 atcttcaggg ctgatgtaga aatgctcaca gactcggccc cagagcatcg cggtcggcac
      661 gtcgcggccg ccgcgctcct tctgcgtctc gtgcagaacg tacagcacga tgcgctcctc
      721 gcgcgtaagc ccgtcgcgga cgtcgggcag caaatcctcg atcgccttca tcgggctaga
      781 acctgaaggt gtagctgacc ttcgccgtcg catcggagaa cgccgagtgg aattcgtcgt
      841 cgtggtcgag gtcctccacg ttgtgattga tcacgaagaa cacctcgcgt cccgcttcgg
      901 gaatccagtg cagccgcatg ttgatgccgc cgacctcgct gacgttgtcg tactgaatca
      961 ggttgaccca ggatatcgtc ggcgagaacg cgaagtcgaa tccggcgcgg aggagccgca
     1021 cttcgaacgc gccattcggc accggcagat cgacgtcgct gtagtcgtag ctcaagctac
     1081 cgcggaagaa cgccgacggg cgccacgtga gctccaagta ccgatcggag cggctgccgc
     1141 cgtagaactt gccgtcggga tactcgacga cgcgcatgaa cgccgcgagc ttgcggtggc
     1201 cgcccgttcc gccctggatg ccgcggctgt ccgcatcata ggtgccgacc ggcacgaaga
     1261 cgccggggta gatctggaac ggctcgatga gccgttccgt gaagtcgctg aacgcgaccg
     1321 tgagcgtgtc gttggtgcgg ttcgtgaacg tcagcggccg caggaagtac gcctcggtct
     1381 tgagcccgcc gccgttcaga taatcgatgc gctggtagtc gaaattcgcg agccacgact
     1441 cgagattgcc gccgctcggc cgcagcatgt agccgacgtt gaagaacgta tcgctgatcc
     1501 cggcgctgtc gaggaagccg agcgccgggt tgaactgcgc gccgtagcgg cggtactgca
     1561 ggatgcctcg cacgccttgc gagctcggaa agcggaggct cgcgccgtag gagtcctggt
     1621 cgccgtcgaa cccgtccgtg tcgctctgct ggaagaacac gtcgccttcg agcgcgcggc
     1681 tgcccggcaa gcgcgagttt cgatattgga agtcgaggcc cgcaagcgcg ttttcggcgc
     1741 cggacgtcgg gctgccgtac gtcgtgatga cgccgacgtt cgattcctcg agcacgccga
     1801 gcttcgcgcg cgcgacggac agcgtgtcgg ccgggatcac gcaagagctc gtggccggca
     1861 cggtcggcag agccgcacac tcgacgccga tcggctcgcg gccccgcaac acggtgacgg
     1921 agtgcgcatc ctggcgaacc gagagacccc cgacctccca gcgtccgacg cgtcccgaga
     1981 ccttgccgcc gtactcgaga tcgacgacct gcccggtcgt gcttaggccg atgcgccgcg
     2041 agaagaacgg ccggccgttt tgcgccagcg tgttgagacc cggtatcgac gactggcgct
     2101 ggccgccgat gccgccgaac tcgaagatgt cggcctcgcg caggaagaag tcgcgtttct
     2161 ccggaaagaa gagacccagg cgcgtgaggt tgacttgccg gtcgtcgacc tcggtcaccg
     2221 agaaatccgt attgatcgtc agcgagccgt tcagctgcgg cgtgatgcga tacgcgaggt
     2281 cgagcgacgg cttcgtctcg gagtcggtcc cgcccgccat gaaatccttg cggtcgctga
     2341 cggcgagcga cggcacgacc tcgagcccaa tgccttgctg cagcccctcg aagccaatcg
     2401 cgagccccga gacggcgggg ttgtaagcgc ggttgcgcga gacccaggcg atgatctcgt
     2461 tcttgcgcac gacggtgcgc gagaagttga ggccccacgt atccgtcgcc ggatcgaacg
     2521 agatcgactt gtacggaatc tccatctcgg ccgtccagcc gccttcgaag cggccggccg
     2581 cggcgtcgta gatcgagtcc cagtcgccgt agaactccgt gacgttgcga taaagcccgt
     2641 cggcgcgcac gccgttcggg ttcagcccga agaaatagcc actgcgccga tcgttgaacg
     2701 ggtcgatcgt gacgtagaag cgatcgtcct ggcccaggtt gtcgttctgg cgcatgttgc
     2761 gcgccgtgat cccgttagga tcgtcgtaga gccgcgccgc gacatagagt gcgtcgtcgt
     2821 cgtataggat caagatctcg gtccgctcgt cgggctcggc atactcgatc ggcgtcactt
     2881 ggtgcagatc gtccacgaca gccgcgtttg cccagaccgc gtcgctcaaa tcgccgtcga
     2941 tgacgggcgg agttgccgtg cgcacgacgc gcacgctctt ttggcccggg tcctcgagca
     3001 ccgcgttgtt ctgcgcggct gcgctgcccg ctgccgcgca cgcgagcgca gcgcagattc
     3061 cctgcttcga tcgattcatg ggggcgacgt tctacaggtg gcgcgtcacc atcgcaacca
     3121 cgtgtcgccg cccaagcacg cgcagcgcca gcatcggcgc tcccactcaa tgttgacagg
     3181 ggctcacgat ccagtcgggg atccactgtg cgatcatgac gagcgccgcg ctgcccgcca
     3241 tcagcatcgc gagcacgccc atgaatcgcg tgcggcccgc gctgccgccg ccgtgatcgg
     3301 gccagccgcc ccccgcggcg cgccactcgt ggaacgcgag ggctccgaac gcgagcgcga
     3361 gcagcacgcc gatcagcgcg gcgatgtgca gcggggcaag atggccgctc gcgcacgcgg
     3421 gctccacgag cacgtatttg atct
//
LOCUS       DASSNN010000455         4790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1827464, whole genome shotgun sequence.
ACCESSION   DASSNN010000455 DASSNN010000000
VERSION     DASSNN010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827464"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..809)
                     /gene="rdgB"
                     /locus_tag="VFL84_08520"
     CDS             complement(204..809)
                     /gene="rdgB"
                     /locus_tag="VFL84_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004958743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HET7608703.1"
                     /translation="MTRRKHWVLATANRGKVAELKALLAEAGLDIEVTPQSELEVAAP
                     PETGATFVENALTKARHAARATGLPAIADDSGLVVPALGGAPGIRSARFAGENADDRA
                     NVAKLLAALEAPSLDRAASFHCVLVALEHAEDPAPLIASGSWAGEIARAPRGRGGFGY
                     DPVFFDPTLGATAAEIEPGMKNRVSHRGTALRALVELLKNR"
     gene            complement(806..1522)
                     /gene="rph"
                     /locus_tag="VFL84_08525"
     CDS             complement(806..1522)
                     /gene="rph"
                     /locus_tag="VFL84_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET7608704.1"
                     /translation="MRPSGRRRDQMREVRLTPHFTRHAEGSVLAEFGDTRVLCTASFE
                     PKVPPFLKGKGQGWVTAEYGMLPRSTHSRTQREAARGTQSGRTLEIQRLIGRSLRAVV
                     DREALGENTITIDCDVLQADGGTRTAAITGGYVALALAAARLVGGGKIARSPIHGQVA
                     AVSVGIFRGQAVLDLDYAEDSEAETDMNVVMNDARGFIEVQGTAEGHALQRGELDGLL
                     DLAVVGIQQLLAAQAAVLSK"
     gene            complement(1594..2340)
                     /locus_tag="VFL84_08530"
     CDS             complement(1594..2340)
                     /locus_tag="VFL84_08530"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET7608705.1"
                     /translation="MRVDHAKLTLPGHRTENQDRVDVVAGNEALLLVVVDGMGGHAHG
                     ARAAEVTVATLREAFAEVPQPVFDPQGFLTLSLARAHDRVVKLGDGVALDHKPRATCA
                     VCLVQDGGAYWAHVGDSRIYQLRGGAIKARTRDHSHVELLLREGLIAEGEMRNHPMRN
                     FVECCLGGDVPLPDMSVTARLKLEPGDVLLVCTDGLWSGLEDADFADASRDAGVPIEQ
                     VIRKLAERAVAANAPHSDNTSVAAVRWDGP"
     gene            2500..3354
                     /locus_tag="VFL84_08535"
     CDS             2500..3354
                     /locus_tag="VFL84_08535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET7608706.1"
                     /translation="MTGFSTAEARVGPFRLVWEIRSVNHRFLELGFRLPEDLRAVEPD
                     CRELVGASIKRGKVDCSLKIGADDRGRVADTLVAGALERLRALEGKVRAVFPDARAMT
                     AEEVLRWPGVLEEPAQNLEELGEAVKDCLGAALRALTDSRAREGARIAEMLEKRNAAI
                     TALLSGIRPQLDGVQLRYRERLRERLERLDVKADPERLEQELALVAQRADVAEEIDRI
                     ASHVAEVEAILKRNEPVGRRLDFVIQELNREANTFASKVQDEALTRVAVELKVLIEQM
                     REQVQNLE"
     gene            3351..3989
                     /gene="gmk"
                     /locus_tag="VFL84_08540"
     CDS             3351..3989
                     /gene="gmk"
                     /locus_tag="VFL84_08540"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET7608707.1"
                     /translation="MSAPATRNGQLWVIAAPSGAGKTSLVKALVERDPSLKFSISYTT
                     RAPRPSEVEGRDYFFVSKEKFIEMANAGAFLEHACVFDNWYGTGREHVAKLLATGSSV
                     VLEIDWQGARQVRERAPDSHSIFILPPSVAELERRLRGRKSDSDAVIARRLKDALGDM
                     THWHEFDHLIVNDDFDRALERLAGVVSGREQGARTSLPAVRAAAQAIVSGGR"
     gene            4062..4340
                     /gene="rpoZ"
                     /locus_tag="VFL84_08545"
     CDS             4062..4340
                     /gene="rpoZ"
                     /locus_tag="VFL84_08545"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET7608708.1"
                     /translation="MARITVEDCLEKVPNMFQLVLVAAKRARQLANGAHPMVDWENDK
                     PTVVALREIAAGHIGPEILEEAERPQPEVMPEIEMSGELRAPHLGLGD"
     gene            4381..>4790
                     /locus_tag="VFL84_08550"
     CDS             4381..>4790
                     /locus_tag="VFL84_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7608709.1"
                     /translation="MDYASSILGLLPSGRRSIGVTQLLNKLETYLPPAQVDRIRDAYE
                     FGAEAHQGQKRLSGEPYIAHPVAVADLLADLHMDAPTIIAAILHDVIEDTPTAKDELA
                     ERFGADVAELVDGVSKLDHIQFRSRAEAQAESFR"
BASE COUNT          763 a         1646 c         1641 g          740 t
ORIGIN      
        1 gccgcggcgc cgtagccgcg ccgacacgat ttgaggcgtt ttggttgaac aatctaaaga
       61 tcgcgtgcct cagcccgttc aatgggttag cgtcgcattt gactccgagc cgctagaaag
      121 gcggcgcaac acgctggttt agatttggcg aaggtgcgcg tgtgcataaa tctcggcccg
      181 aaaccagctc accgcgcgcc aacctaccga ttcttcaaga gttcgacgag cgcccgtaac
      241 gccgtcccgc ggtggctcac gcgatttttc atgcccggct cgatctcggc ggccgttgcg
      301 ccaagcgtgg gatcgaagaa aacggggtcg tagccgaagc cgccgcggcc gcgcggggcg
      361 cgggcgatct cgcccgccca cgatccgctc gcgatcaacg gcgcggggtc ctcggcgtgc
      421 tcgagagcga cgagcacgca gtgaaagctc gcggcgcggt cgagcgacgg cgcctcgagc
      481 gccgcgagca gctttgcgac gttcgcgcgg tcgtccgcgt tctccccggc gaatcgcgcg
      541 gaccgaatgc cgggcgcgcc gccgagcgcg gggacgacga gacccgagtc gtcggcgatc
      601 gcgggcagcc ccgtcgcgcg cgcggcgtgg cgcgccttcg tcagcgcgtt ctcgacgaac
      661 gtcgcgccgg tctccggcgg cgcggcgact tcgagctccg attgcggcgt cacctcgata
      721 tcgagacccg cttcggcgag cagagcctta agctccgcga ccttgccgcg gttcgccgtc
      781 gcgagcaccc agtgcttgcg ccgcgtcact tcgagaggac ggcggcctgc gcggcgagca
      841 gttgctggat gccgacgacg gcgaggtcga gcagcccgtc gagctcgccg cgctgcaacg
      901 cgtggccttc ggccgttccc tgcacctcga tgaaccccct cgcgtcgttc atgacgacgt
      961 tcatgtcggt ctccgcttcg gaatcctcgg cgtagtcgag gtcgagcacg gcctggccgc
     1021 gaaagatgcc gaccgagacg gccgcgactt ggccatgaat gggactgcgc gcgattttgc
     1081 cgccgccgac gagccgcgcc gcggcgagcg ccaacgcgac gtagccgccc gtgatcgcgg
     1141 ccgtgcgcgt gccgccgtcg gcttgcagga cgtcgcagtc gatcgtgatc gtgttctcgc
     1201 cgagcgcctc gcggtcgacc acggcgcgta ggctgcgacc gatgagccgc tgaatctcga
     1261 gcgtgcggcc gctttgcgtg ccgcgcgcgg cctcgcgttg cgtgcggcta tgcgtcgagc
     1321 gcggcagcat gccgtactcg gccgttaccc agccttgtcc cttgcctttg agaaacggcg
     1381 gcaccttcgg ctcgaacgac gccgtgcaca gaacgcgcgt gtcgccgaac tccgcgagga
     1441 ccgatccttc ggcgtggcgc gtgaagtgcg gggtcagccg cacctcgcgc atttggtcgc
     1501 gccggcgtcc gctcggccgc atgagaagct ccgttcgaag cgaggggttc gcggacagct
     1561 taccagctcg cgtagcctcg agcggcggcc gcgctagggg ccgtcccagc gcaccgcggc
     1621 gacgctcgtg ttgtcgctgt gcggcgcgtt cgcggcaacc gctcgttcgg cgagcttgcg
     1681 aatgacctgc tcgatcggaa cgcccgcgtc gcgcgaagca tcggcgaagt cggcgtcctc
     1741 gagcccggac cagaggccgt ccgtgcagac gagcagcacg tccccgggct cgagcttcaa
     1801 gcgagccgtc acgctcatgt ccggcagcgg cacgtcgccg cccaagcagc actcgacgaa
     1861 gttgcgcatc gggtgattgc gcatctcgcc ttcggcgatc aacccctcgc gcagcaacag
     1921 ctcgacatgg ctgtggtcgc gcgtgcgcgc cttgatcgcg ccgccgcgca gctgatagat
     1981 ccggctgtcg ccgacgtgcg cccagtaggc gccgccatcc tgcacgaggc agacggcgca
     2041 ggtcgcgcgc ggtttgtggt cgagcgcgac gccgtcgccg agcttgacga cacgatcatg
     2101 cgcgcgagcc aacgacaagg tcaggaatcc ctgcgggtcg aataccggct gcggaacctc
     2161 ggcgaaggct tccctgagcg tcgcgaccgt cacctcggcg gcgcgcgcgc cgtgcgcgtg
     2221 tccccccatg ccgtcgacga cgaccaagag cagcgcctcg ttgcccgcga cgacgtcgac
     2281 tcgatcctgg ttttccgtcc gatgacccgg cagagtcagc tttgcatgat cgacgcgcac
     2341 tcttcgttac cgctccgctg attctctaca cactggaagc gccttccgcg cttggcgcgc
     2401 agccgggccg caaagtcgct gatgtacact cggttccgcg gcgcgaacgg cgccagattg
     2461 tctgaaacca cctccggact caatcgaatg cctcacagca tgaccggatt ctcgaccgcg
     2521 gaagcgcgcg tggggccgtt ccggctggtt tgggagattc gcagcgtcaa ccaccgattc
     2581 ttggagctcg gcttcaggct gccggaggac ttgcgcgccg tggagccgga ctgccgcgag
     2641 ctggtaggcg cttcgatcaa gcgcggcaag gtcgattgct cgctgaagat cggcgccgac
     2701 gaccgcgggc gcgtcgcgga cacgctcgtc gccggagccc tcgagcggct cagggcgctc
     2761 gagggcaagg tccgcgccgt atttcccgac gcccgggcga tgacggccga ggaggtgctg
     2821 cggtggcccg gagtgctcga ggagcccgcg caaaacctcg aggagctcgg cgaagccgtc
     2881 aaggactgtc tcggcgcggc gctgcgcgcg ctgaccgact cgcgcgcccg cgagggcgcg
     2941 cggatcgccg agatgctcga gaagcgcaat gcggcgatca ccgcgctcct gtcggggatc
     3001 cggccgcagc tcgacggcgt gcagctgcgc tatcgcgaac ggctgcgcga gcgtctcgag
     3061 cggctcgacg tcaaggccga ccccgagcgc ttggagcagg agctcgcgct cgtcgcgcag
     3121 cgcgccgacg tcgccgagga gatcgaccgc atcgcgagcc acgtcgcgga agtcgaagcg
     3181 atcttgaagc gcaacgagcc cgtcggccgg cggctcgact tcgtcattca ggagctgaat
     3241 cgcgaagcga acacgttcgc atcgaaggtc caggacgagg cgttgacgcg cgtggccgtc
     3301 gagctgaagg tgctgatcga gcagatgcgc gagcaggtgc agaatctcga atgagcgcgc
     3361 cggcaacgcg aaacggccag ctctgggtca tcgcggcgcc gtccggcgcc ggcaagacgt
     3421 cgctcgtcaa ggcgctcgtc gagcgcgatc cttcgctcaa attctcgatc tcgtacacga
     3481 cgcgcgcgcc gcgcccttcg gaggtcgagg gccgcgacta ttttttcgtg tcgaaagaaa
     3541 aattcatcga gatggcgaac gccggcgcgt tcttggagca cgcgtgcgtg ttcgacaatt
     3601 ggtacggaac gggacgcgag cacgtcgcaa agctgctcgc gaccgggtcg tccgtcgtgc
     3661 tcgagatcga ctggcaaggc gcgcggcagg tccgcgagcg cgcgccagac tcgcacagca
     3721 tcttcatcct gccgccgagc gtggccgagc tcgagcggcg gttgcgcggg cggaagtccg
     3781 attcggatgc cgtcatcgcg cgcaggctca aggacgcgct cggcgacatg acgcactggc
     3841 acgagttcga tcacctcatc gtcaacgacg acttcgaccg ggcgctcgag cgcttggcgg
     3901 gagtcgtctc gggccgcgag caaggcgcgc gcacgagcct gccggccgtg cgtgcggccg
     3961 cgcaggccat cgtttccggg ggccgctgac gcccgcactt gctgggccta gggggtgctg
     4021 cattagactt acgggttcta agccccggag ggccgtccgc aatggcaaga atcaccgtcg
     4081 aagattgttt ggaaaaagtc ccgaacatgt tccagctcgt gctcgtcgcg gcgaagcgcg
     4141 cgcgccagct tgccaacggc gcccatccga tggtcgattg ggagaacgac aagccgaccg
     4201 tcgtggcgct gcgcgagatc gcggccggcc acatcggccc ggagattctc gaagaggccg
     4261 agcgtccgca gcccgaagtc atgccggaga tcgaaatgtc cggcgagctg cgcgccccgc
     4321 acctcggatt gggagactga ccgctcccca accggccggc ccgatcggcg cgcttcgcca
     4381 atggactacg cgtcttccat cctggggctg ctgccgagtg gaagacgctc cataggcgtc
     4441 acgcaactgc tgaacaagct cgagacctac ctgccgccgg cgcaggtgga ccggatccgc
     4501 gacgcgtacg agttcggcgc cgaggcccac cagggccaga agcggctgtc cggcgagccc
     4561 tacatcgccc acccggtcgc cgtcgccgac ctgctcgccg acctgcacat ggacgcgccg
     4621 acgatcatcg cggcgatcct gcacgacgtc atcgaggaca cgccgaccgc caaggacgag
     4681 ctggccgagc gcttcggcgc cgacgtcgcc gagctggtcg acggcgtctc gaagctcgat
     4741 cacatccagt tccgcagccg cgccgaggcg caggccgaga gcttccgcaa
//
LOCUS       DASSNN010000456         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_584423, whole genome shotgun sequence.
ACCESSION   DASSNN010000456 DASSNN010000000
VERSION     DASSNN010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584423"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VFL84_08555"
     CDS             <1..718
                     /locus_tag="VFL84_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET7608710.1"
                     /translation="HAVTQFRVGDEVFGASKFGAFAELVCVGEDTLVPKPAGVSFEAA
                     AATPTAAYTALQGLQKGRIAAGQRVLIDGASGGVGTFAIQIAKAYGADVTAVCSTRNV
                     EIARALGASRVIDYRREDLASDAQRYDLVLAANAYRPLSHYRRILAPNGAYVMTGGGG
                     VQILQAMLLGAVISMTSSTRISNIMAVAKKKDLVVLKELLETGKIKPVIDRTVALGAV
                     PDAIRYLEQHHARGKVVVSI"
     gene            774..1925
                     /gene="prpC"
                     /locus_tag="VFL84_08560"
     CDS             774..1925
                     /gene="prpC"
                     /locus_tag="VFL84_08560"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HET7608711.1"
                     /translation="MSAETPTPARGKKSVALSGVVAANTALCTVGRTGNDLHYRGYDI
                     LDVAETCEFEEIAHLLVHERLPTSAELAAYRQKLKSLRELPEQLLDVLEAIPASAHPM
                     DVLRTGVSMLGCVEPERDQTPAHARDIADRLIASLPGMLLYWHHFTRNGVRLKLESGA
                     DSVAAHFLTLLHRRPPPESWVRAMHTSLVLYAEHELNASTFTARVIAGTGSDLYSCIA
                     GGIGALRGPKHGGANEVALEIQERYRSPDEAVADIRARLARKEVVIGFGHPVYTVADP
                     RNRVIKDVARRLSAEVGDDTLFRIADAIEAEMAAAKKMFANLDWFSAVAYHVMGVPTA
                     MFTPLFVMARTTGWSAHVIEQRADGKIIRPSANYVGPENRRFVPLAERG"
     gene            complement(1935..>2328)
                     /locus_tag="VFL84_08565"
     CDS             complement(1935..>2328)
                     /locus_tag="VFL84_08565"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET7608712.1"
                     /translation="CGFTPQYKGLEALWRKRRAEGFAVLGFPCDQFGQQEPGDEAEIK
                     QFCSLTYDVTFPLFAKIEVNGAGAHPLYLWLKGEKAGVFGTEAIKWNFTKFLIDRNGQ
                     VAKRFAPADSPEKIDKTVGELLAAPARS"
BASE COUNT          391 a          780 c          757 g          400 t
ORIGIN      
        1 ccacgcggta acgcagttcc gcgtcggcga cgaggtgttc ggcgcgagca agtttggcgc
       61 ctttgccgag ctcgtctgcg tcggcgagga cacgctcgtc ccgaagcccg ccggcgtctc
      121 cttcgaggcg gcggccgcga cgccgacggc cgcctacacg gcgctccagg gccttcagaa
      181 aggacgtatc gcggcgggac agagagtgct gatcgacggc gcgtccggcg gcgtcggaac
      241 gttcgcgatc cagattgcga aagcttatgg cgcggacgtg acggccgtgt gcagcacgag
      301 aaacgtcgag attgcgcgcg cgctcggcgc gtctcgcgtc atcgactatc gccgcgagga
      361 cctcgcgagc gacgctcagc gctacgatct cgtcttggcg gcgaatgcct atcgtccgct
      421 gtcccactac aggcgcatcc tcgcgcccaa tggcgcctac gtcatgaccg gcggcggcgg
      481 cgtccagatc ttgcaggcga tgctgctggg cgccgtcata tcgatgacga gcagtacgcg
      541 aatcagcaac atcatggcgg ttgcgaagaa gaaggatctc gtcgtcctca aagagctgct
      601 cgagacggga aagataaagc cggtcatcga ccgaaccgtt gcgctcggcg ccgtgcccga
      661 cgcgatccgt tatctcgagc agcatcacgc gcgaggcaag gtcgtagtca gcatctagcg
      721 gtttgccgcg tcgtgatttt gttccgcgcc tcgtttggcg tagtgtcggg cccatgagcg
      781 ctgaaacacc cacgcccgcg cgaggcaaga aatcggttgc actatccggc gtcgtcgccg
      841 cgaacacggc gctgtgcacg gtcgggcgaa ccggcaacga cctgcattac cgcggctacg
      901 acatcctcga cgtggccgag acctgcgagt tcgaggagat tgcgcacctg ctcgtccacg
      961 agcggctgcc aacctcggcg gagctcgccg cgtaccgtca aaaactcaag tctctccgtg
     1021 agttaccgga gcaacttctc gatgttttgg aagccatccc ggcgtccgct cacccgatgg
     1081 acgtgctgcg gaccggcgtg tcgatgctcg gctgcgtcga gcccgagcgc gaccaaacgc
     1141 ccgcgcacgc gcgcgacatc gcggaccggc tcatcgcgtc gctgcccggc atgctgctct
     1201 actggcacca cttcacacgc aacggcgtgc ggctcaagct cgagtcgggc gccgactccg
     1261 tcgcggcgca tttcctgacg ctgctgcatc ggcgccctcc tcccgaatcg tgggtccgcg
     1321 cgatgcacac gtcgctcgtg ctctacgccg agcacgagct caacgcttcg acgttcacgg
     1381 cgcgcgtcat cgcgggcacg ggctccgacc tctactcgtg catcgcgggc ggcatcggcg
     1441 cgctgcgcgg accgaagcac ggcggcgcga acgaggtcgc gctcgagatc caggagcgct
     1501 accgctcgcc cgatgaagcc gtcgccgaca tccgcgcacg cctcgcgcgc aaagaggtcg
     1561 tcatcggctt tgggcatccc gtctacacgg tggccgatcc gcgcaatcgc gtcatcaagg
     1621 acgtcgcccg gcgcctgtcg gccgaggtcg gcgacgatac gttgttccgc atcgccgacg
     1681 cgatcgaggc ggagatggcc gccgcgaaaa agatgttcgc gaacctcgac tggttctcgg
     1741 cggtcgccta tcacgtcatg ggcgtgccga cggcgatgtt cacgccgctg ttcgtcatgg
     1801 cacgcacgac cggttggtcc gcgcacgtca tcgagcagcg cgccgacggc aagatcattc
     1861 ggccgagcgc gaactacgta ggcccggaga accgccgctt cgtcccgctc gccgagcgcg
     1921 gctgaagcag ctgattacga gcgcgccggc gcggcgagca gctcgccgac ggttttgtcg
     1981 atcttttcgg gcgaatcggc gggcgcgaag cgcttggcga cttgcccgtt gcgatcgatg
     2041 agaaacttcg tgaagttcca cttgatcgcc tcggtgccga acacgccggc cttctcgcct
     2101 ttgagccaga ggtaaagcgg atgcgcgccg gcgccgttga cctcgatctt cgcgaacagc
     2161 gggaatgtga cgtcgtaggt gagcgagcag aactgcttga tttccgcttc gtcgccgggc
     2221 tcctgttggc cgaactgatc gcacggaaaa ccgagcacgg cgaaaccttc ggcgcgccgt
     2281 ttgcgccata gcgcttcgag ccctttgtac tgcggcgtga agccgcat
//
LOCUS       DASSNN010000457         5153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_210270, whole genome shotgun sequence.
ACCESSION   DASSNN010000457 DASSNN010000000
VERSION     DASSNN010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210270"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..773
                     /locus_tag="VFL84_08570"
     CDS             93..773
                     /locus_tag="VFL84_08570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET7608713.1"
                     /translation="MMAQSVYAVLRLAVVLVLTAGGAASAQAQRPLGPLDDGVVKYFI
                     APPLEDAAARPADTDLAVWAFEEWQRAVGGALKLVRVANESEALVRLYWVPAAGGQYG
                     EMRPLMVEGRRGAAVYIRPDTTALGPDIAARARLDALFRDTIVYLTCVHELGHAFGLS
                     HTDEFEDIMYFFGFGGDIVEFFARYRRQLKVRDDIRSASGLSEGDLMQLGALYGRSST
                     PGQLTALN"
     gene            898..1416
                     /gene="infC"
                     /locus_tag="VFL84_08575"
     CDS             898..1416
                     /gene="infC"
                     /locus_tag="VFL84_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET7608714.1"
                     /translation="MVQGKRVRRNAEILSPRVRVIGPNGDQLGIMTVQEALRSAESSG
                     LDLVEVAPGAEPPVCRIMDYGKFVFEQNKKAQSARRKQKQIQVKEVKFRPGTEEGDYQ
                     VKLRNLIKFLTHGDKAKVTLRFRGREMVHQELGAELLKRVQTDLEEYGSVEQQPLMEG
                     RQMVMVIGPRKR"
     gene            1524..1721
                     /gene="rpmI"
                     /locus_tag="VFL84_08580"
     CDS             1524..1721
                     /gene="rpmI"
                     /locus_tag="VFL84_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET7608715.1"
                     /translation="MPKLKTKRGAAKRFRAKGNGGFKSGHAHMRHILTKKSPKRKRKL
                     TRTMQVHDSDVEGLRRLLPYV"
     gene            1732..2091
                     /gene="rplT"
                     /locus_tag="VFL84_08585"
     CDS             1732..2091
                     /gene="rplT"
                     /locus_tag="VFL84_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007420413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET7608716.1"
                     /translation="MARVKRGVTAKARHRKILDQAKGYYNARRKVFRAAKQAVIKAGQ
                     YSFRDRRVRKREFRALWITRINAAARANGLSYSRLMNGLALAHIDVDRKILADIAVHD
                     NEGFAALVAAAKEALAA"
     gene            2114..3097
                     /gene="pheS"
                     /locus_tag="VFL84_08590"
     CDS             2114..3097
                     /gene="pheS"
                     /locus_tag="VFL84_08590"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET7608717.1"
                     /translation="MSSVAELLGQAEAAVRAAEDLAALDAVRVQFLGKKGVLTAQLRE
                     LGKLPPEEIRAAGKAINDAKSALTAAIDSRRDALEARALEATLKAEALDVTLPGRGAV
                     PGQLHPVTRSLRRIVAILSHAGFDVHTGPEVEDDFHNFTALNIPDNHPARAMHDTFYL
                     RSGLLLRTHTSPVQIRALKEHGAPIRLIAPGRVYRRDSDLTHTPMFTQVEGLVVDRRV
                     SFANLKALLYDFSSKFFEREVELRFRPSYFPFTEPSAEVDVRSESGRWLEILGCGMVH
                     PNVLRNAGVDPTEWSGYAFGMGVERLTMLRYGVDDLRAFFENDLRFLEQLA"
     gene            3108..>5153
                     /gene="pheT"
                     /locus_tag="VFL84_08595"
     CDS             3108..>5153
                     /gene="pheT"
                     /locus_tag="VFL84_08595"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET7608718.1"
                     /translation="MRVNLEWLRDWVEIGDADSVAADLTTSGLEVESIEPLANLDRNI
                     VVAEVLSVERHPNADRLSVCSVDDGAGRHQVVCGAPNVAPGIKAPFARVGSQLPGGKA
                     IGAAELRGVKSNGMLCSGKELALADDGAGLLLLDADAPTGTSLTDYLRLDDAALEVNV
                     TPNRGDCFSVIGLARELAARRDTALRGPAIAAVRATVKDTVAISLGAKTACPRFAGRV
                     LTGLSRTARTPLWMRERLRRAGVRPLQPIVDVTNYVMLELGQPMHAYDLAKLDGRIEA
                     RLARAGEKLTLLDGREVALADDMLVIADARGPVGLAGVMGGQSTAVGSTTDSIFLESA
                     FFAPGAIAGRPRRLGLHTDASLRFERGVDPSGQVRAIERATELLLEICGGKAGPVSDV
                     KHRPGLPKRSAVTLRRERLGAVLGVDVPAKRVAKILERLEMTTKPTAAGWRVTPPAFR
                     FDIAIEEDLIEEVGRLFGYDAIPATPGEVVERLGLASEKAVEADDIADLLAARGYAEA
                     ITYSFVDAALDALVEPGSPPVALANPIASDMAVLRQSLWPGLINAARLNVTHQRQRLR
                     LFEIGPQFAAAGQGVTQHGVVAGLALGTRAAEHWDGAGPEVDYFDVKGDVEAVLRLTG
                     AAGEFHFEAATHPALSPGRTARIVRGDRTAGWLGALHPELAKRIDKKRSAVVFALQLD
                     "
BASE COUNT          852 a         1727 c         1792 g          782 t
ORIGIN      
        1 tgacggcgca gccgacgagc cgaatgcacg ctccaaacga atcagggccc ctgcgttgac
       61 tcgccgcgcc ggccggggca gggtgcttgc gcatgatggc gcaatcggtc tatgcggtcc
      121 tgcggctggc ggtggtgctg gtcctcacgg cgggcggcgc ggcgagcgcg caagcgcagc
      181 ggccgctcgg accgctcgac gacggcgtcg tcaaatattt catcgcgccg ccgctcgagg
      241 atgccgcggc ccggcccgcc gacacggacc tcgcggtctg ggcgttcgag gaatggcagc
      301 gcgccgtggg cggcgccctc aagctggtgc gcgtcgcgaa cgaaagcgag gcgctcgtga
      361 ggctctattg ggttccggcg gccggcggcc agtacggcga gatgcggccg ctcatggtcg
      421 aaggacggcg cggcgcggcc gtctacatcc gcccggacac gacggctctc ggccccgaca
      481 tcgcggcgcg cgcgcggctc gacgccctgt tccgcgacac gatcgtctac ctgacctgcg
      541 tccacgagct cggccacgcg ttcggcctgt ctcatacgga cgagttcgaa gacatcatgt
      601 atttcttcgg cttcggcggg gacatcgtcg agttcttcgc gcgctaccgc cggcagctga
      661 aagtgcgcga cgatatccgc tcggcgtcgg ggttgtcgga aggcgacctc atgcagcttg
      721 gggcgctcta cggccgttcc tcgacaccgg gtcagctaac ggcattgaac tagctgaaaa
      781 ctaaggcgtt tgccacgcgc gcgccttgtg ccgattttcg cgggcccgta caatgccgcg
      841 ctggcctgcg accgggaggt tgccggccgc acttttttta accggaagga gtcacgaatc
      901 gtccaaggaa agcgcgtcag acgcaatgcg gagatcctat cgccgcgtgt tcgcgtcatc
      961 ggccccaacg gggaccagct cggaatcatg accgttcaag aggcgctgcg aagcgccgaa
     1021 agttcaggtt tggatctcgt cgaggttgcg cccggcgccg agcctcctgt ctgccgaatc
     1081 atggactacg gcaagttcgt gttcgagcag aacaagaagg ctcagtctgc gcggcgcaag
     1141 cagaagcaga tccaagtcaa ggaagtgaaa tttcgacccg gcaccgaaga gggcgactat
     1201 caggtcaagc tgcgaaatct catcaagttc ctgacgcacg gcgacaaagc caaggtcacg
     1261 ttgagattcc ggggacgcga gatggtgcac caagagctcg gcgcggagct gctgaagcgg
     1321 gtgcaaaccg acttggaaga gtacggctcg gtagagcagc agccgctcat ggaagggcgg
     1381 caaatggtca tggtgatcgg cccgcgcaag cgctaagccc gtcgccctaa aacgaacaag
     1441 tgctcgttcg cggcaaagac gccgcgggga gccgcccgcc cagtcgaggc aacactggaa
     1501 cgggctaaca cgaggagaaa gtcatgccga agctgaagac gaagcgcggc gctgcgaagc
     1561 gtttccgcgc gaaaggtaat ggcggattca agtccggcca tgcgcacatg cgccacatcc
     1621 tgaccaagaa gtcgcccaag cggaaacgca agttgacgcg cacgatgcag gtccacgact
     1681 ccgacgtcga ggggctgcgc cggttgctgc cgtacgtttg aggagactta gatggccaga
     1741 gtaaaacgag gcgttaccgc caaggcgcgc caccgcaaga tcctcgatca ggcgaagggc
     1801 tactacaacg cgcggcgcaa agtgtttcgc gccgcgaagc aggccgtcat caaggccggc
     1861 cagtattcgt tccgcgaccg ccgcgtgcgc aaacgcgagt tccgcgctct gtggatcacg
     1921 cgcatcaacg ctgcggcccg cgcgaacggg ctgtcgtaca gccgcttgat gaacggcttg
     1981 gcgctcgcgc acatcgacgt cgatcgcaag atcctcgccg acatcgccgt gcacgacaac
     2041 gaaggctttg ccgctctcgt cgcggccgcg aaagaggctc tcgccgccta accacgcact
     2101 cggggagctc gcggtgagca gcgtcgcgga actgctgggc caggccgaag cggccgtgcg
     2161 cgcggccgaa gacttggccg cgctcgacgc cgtgcgcgtg caattcctcg gcaagaaggg
     2221 cgtgctcacg gcgcagctgc gcgagctcgg caagctgccg cccgaggaaa ttcgcgcagc
     2281 cggcaaggcg atcaacgacg cgaagtcggc gctgacggcc gccatcgact cgcgccgcga
     2341 cgcgctcgag gcgcgcgcgc tcgaggccac gctcaaagcc gaggcgctcg acgtcacgct
     2401 gccgggccgc ggggcagtgc ccggtcagct gcaccccgtc acacggagct tgcggcgcat
     2461 cgtcgcgatc ctgagccacg ccggcttcga cgtgcacacg gggcccgagg tcgaggacga
     2521 ctttcacaac ttcacggcgc tgaatatccc ggacaatcac cccgcgcgcg cgatgcacga
     2581 cacgttctac ttgcgctccg gcctcttgct gcgaacgcac acgtcgcccg tgcagattcg
     2641 cgcgctcaag gagcacggcg cgccgatccg gctcatcgcg ccgggccgcg tctatcgccg
     2701 cgattccgat ctcacgcaca cgccgatgtt cacgcaggtc gaagggctcg tcgtcgaccg
     2761 gcgcgtcagc ttcgcgaact tgaaggcgct gctctacgac ttctcgtcga agttcttcga
     2821 gcgcgaggtc gagctgcgct ttcggccgtc gtactttccg ttcacggagc cgtcggccga
     2881 ggtcgacgtg cggtcggagt ccggccgttg gctcgagatc ttgggctgcg gaatggtgca
     2941 tccgaacgtg ctgcgcaacg cgggcgtcga tccgaccgag tggagcggct atgcgttcgg
     3001 catgggcgtc gagcggctga cgatgctgcg ctacggcgtc gacgacttgc gcgcgttctt
     3061 cgagaacgac ctgcgctttc tcgagcagct cgcctgaggc gtaagtcatg cgcgtaaatc
     3121 tcgaatggct gcgggattgg gtcgagatcg gcgacgcgga tagcgtcgcc gcggacctca
     3181 cgacgtcggg gctcgaggtc gagtcgatcg agccgctcgc gaacctcgac cgcaacatcg
     3241 tcgtggccga ggtgctatcc gtcgagcggc atccgaacgc ggaccgcctg tcggtctgca
     3301 gcgtcgacga cggcgccggc cggcaccagg tcgtctgcgg cgcgccgaac gtcgcacccg
     3361 gcatcaaggc gccgttcgcg cgcgtcggct cgcaattgcc gggcggcaaa gcgatcggtg
     3421 cggcggagct gcgcggcgtc aaatcgaacg gcatgctctg ctcgggcaag gagctcgcgc
     3481 tcgccgacga cggcgccggc ctgctgctgc tcgacgccga cgcgccgacg ggcacgagct
     3541 tgaccgacta tttgaggctc gacgacgccg cgctcgaggt caacgtgacg ccgaaccgcg
     3601 gcgattgctt cagcgtcatc ggcctggcgc gcgagctcgc cgcccggcgc gatacggcac
     3661 tgcgcgggcc cgcgatcgcc gccgtgcgcg cgaccgtcaa ggataccgtc gcgatctcgc
     3721 tcggcgcgaa aaccgcgtgc ccgcgcttcg ccggccgcgt gctgacggga ctttcgcgca
     3781 cggcgcgaac gccgttgtgg atgcgcgagc ggctgcgccg ggccggagtt cgcccgctgc
     3841 agccgatcgt cgacgtgacg aactacgtga tgctcgagct cggccagccg atgcatgcct
     3901 acgacctcgc gaagctcgac gggcgcatcg aggcgcggct cgctcgcgcc ggcgagaagc
     3961 tcacgctgct cgacggccgc gaggtcgcgc tcgccgacga catgctcgtg atcgcggacg
     4021 cacgcggccc tgtcggcttg gccggcgtca tgggcggcca gtcgacggcc gtcggcagca
     4081 cgacggattc gatttttctc gagagcgcct tcttcgcgcc cggcgcgatc gcggggcgcc
     4141 cacggcggct cggcttgcac acggacgcgt cgcttcgctt cgagcgcggc gtcgatccgt
     4201 cgggtcaagt gcgcgcgatc gagcgcgcca ccgagctgct gctcgagatc tgcggcggca
     4261 aggcggggcc ggtctcggac gtcaagcacc ggccgggatt gccgaagcgc tcggcggtca
     4321 cgttgcggcg cgagcgcctc ggcgccgttc tcggcgtcga cgtgcccgcc aaacgcgtgg
     4381 ccaagatcct cgagcggctc gagatgacga cgaagccgac ggccgccggc tggcgcgtga
     4441 cgccgcccgc gtttcggttc gacatcgcaa tcgaggagga cttgatcgag gaggtcggcc
     4501 gcctgttcgg ctacgacgcg attcccgcca cgcccggcga ggtcgtcgag cggctggggc
     4561 tcgcgagcga gaaggccgtc gaggccgacg acatcgcgga tttgctcgcg gcgcgcggct
     4621 acgccgaggc gatcacctac agcttcgtcg acgccgcgct cgacgcgctc gtggagcccg
     4681 gttcgccgcc cgtcgcgctc gcgaatccga tcgcgagcga catggcggtg ctgcgccaat
     4741 cgctgtggcc cgggctcatc aacgcggcgc ggctcaacgt gacgcatcag cgccagcggc
     4801 tgcggctgtt cgagatcggg ccgcagttcg cggcggccgg gcagggcgtt acacagcacg
     4861 gcgtcgtcgc gggcctcgcc ctcggcacgc gcgcggccga gcactgggat ggcgccggac
     4921 ccgaggtcga ctacttcgac gtcaaaggcg acgtcgaggc ggtgctgcgg ctcacgggag
     4981 cggccggcga atttcacttc gaggccgcga cgcacccggc gttgagcccg gggcgcacgg
     5041 cgcggatcgt gcgcggcgac cggaccgcgg gttggctcgg cgcgctgcac cccgagctcg
     5101 cgaagcgcat cgacaaaaag cgcagcgcag tcgtgttcgc gcttcagctc gac
//
LOCUS       DASSNN010000458         3292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1827992, whole genome shotgun sequence.
ACCESSION   DASSNN010000458 DASSNN010000000
VERSION     DASSNN010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827992"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFL84_08600"
     CDS             <1..289
                     /locus_tag="VFL84_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HET7608719.1"
                     /translation="EIYSLFPATCADAYGRKYAAGNYGLLYTAKGTASLLVPVTSVMA
                     AASGGWHTVFIAAAAMNLVAAFMALFVLRPLRLRMHAARVADALGASPHPG"
     gene            complement(327..1001)
                     /locus_tag="VFL84_08605"
     CDS             complement(327..1001)
                     /locus_tag="VFL84_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7608720.1"
                     /translation="MAVQPFRATFSAAALLAAGQFPAPAYAEGPMSWSVGGATDETHV
                     EVLRDGWGYESSGSQRGFSARGALELSRNFELELGAMSASDLHWTEYFSGYQGYLTAH
                     TTFDVRALNVSAVGKVVGRTFEGYIKAGVARYDIDGSQVLDTLLTDAAATRDVHASDF
                     DYLLGAGFFIKPAPKWRVRVEYQYFAVERDFLGISSDDDPSVDTFSIGFDYRLPKRQT
                     SQSSPQ"
     gene            complement(1135..1758)
                     /locus_tag="VFL84_08610"
     CDS             complement(1135..1758)
                     /locus_tag="VFL84_08610"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7608721.1"
                     /translation="MTDYARLALLATLAAAAPPALAQTFAGNEIVAGAAAELATTIAT
                     RRAELEDDHEAIAKLVDTLILPRFDMRRGSRAILAEHWDSASAADRDRFVTAFYDYLV
                     ATYGDLLLQFKPETLRVLPFDGDAAHSPARVHTIITLTDGTEVDVDFVMIGAGQDWRV
                     VDIEAEGVSYVKTYRSQFRVDIAADGLGSVIEWLEQKAAAVRAPGTR"
     gene            complement(1751..>3292)
                     /locus_tag="VFL84_08615"
     CDS             complement(1751..>3292)
                     /locus_tag="VFL84_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608722.1"
                     /translation="NPVVIGGGIAAAIVLAGIALWAMGRGTAESGGTDAVARNTIAVL
                     PFTVGGSPDAAYFGEGLADEILNRLSAVDGLQVAARTSSFAARASTDDVRSIGQRLAV
                     AHLVEGTVRRDGERVKIDVQLVDARRGYRIWNAPYEGVFGDIFALQDEIANTIVAKLR
                     DTVPTELPTAPIATSAPTTNLDAYDRLLEGRRYLNRRDEDSLRRSILLFEQAIELDPR
                     YGQAYVELAKAYVLLPSYSAEIQDEMFDLALATLAKGLANNASIDDSMQVVIALVSYA
                     RWDWIAAEVAFRRALEHERNDSDLLVWYSQFLAAVGRPEASAEHARRARQLDRLSPVV
                     THRLSVASMWIDADDDAARYALVAEELGMAPTPDAYIVLKLRERDYAAVRPLLIGVQT
                     MFARPTAWVDPLLDGLAHPERRAQAVAALARAEQARDIPPKYLFGSWLYLGEPERAID
                     AGLRLVNDRPSFNVEFVFAREAHQLRQHPRFGELVRAIGLNRYWDRFGWPETCRHSGD
                     TITCD"
BASE COUNT          548 a         1084 c         1120 g          540 t
ORIGIN      
        1 cgagatctac agcttatttc cggcgacgtg tgcagacgcc tacggccgca agtacgcggc
       61 aggcaattac gggctcttgt ataccgctaa aggcaccgcc tcgctgctag tgccggttac
      121 gagtgtcatg gcggcggcat cagggggctg gcacacggtg ttcatagcgg ctgcggcgat
      181 gaatctcgtc gcggcgttca tggcgttgtt cgtgctgagg ccgttgcgac tgcgcatgca
      241 cgccgcgcga gtagccgacg ccctcggcgc cagcccgcac ccggggtagc cgacaatcta
      301 gtgctccagt tatctcgcgc tggaggttac tgcggcgagc tttggctcgt ctgccgcttg
      361 ggcagcctgt aatcgaagcc gatcgaaaac gtgtcgacgc tcgggtcgtc gtcgctcgag
      421 atgccgagga agtcgcgctc gacggcgaaa tactggtatt cgactcgcac tcgccacttc
      481 ggcgccggct tgatgaagaa gcctgcgccg agcaggtagt cgaagtcgct ggcatggacg
      541 tcgcgcgttg ccgccgcatc ggtcagtagc gtgtcgagca cttgcgagcc gtcgatgtcg
      601 taccgcgcga cgccggcttt gatgtagccc tcgaacgtgc ggcccaccac cttgcccacg
      661 gcactgacat tcagggctcg cacatcgaaa gtcgtatgag ccgtcagata gccttggtag
      721 cccgaaaagt attcggtcca gtgcagatcc gatgcgctca tggccccgag ctcgagctcg
      781 aagttgcggc tgagctccaa ggcgccgcgt gcggaaaagc cgcgctggga gccgctcgac
      841 tcgtaccccc agccatcgcg aaggacctcg acatgggtct catccgtcgc gccaccgact
      901 gaccagctca tagggccctc ggcgtaggcg ggcgccggga attggccggc cgccagcagg
      961 gcggccgccg aaaaagtcgc gcggaatggt tgaacggcca tgggttgtgc tcctctcgtg
     1021 gtatcgatgg gagccccatg gtctctagcg actcccgcag tggtcctgcg gaaaagccga
     1081 ggttcagctg tggttttgcc gcgaagcggc gggaagcggc ctttcggcgc ggtttcaacg
     1141 cgttcccggt gcccgaacgg ccgcggcctt ttgctcgagc cattcgatga cgctgccgag
     1201 gccgtccgcg gcgatgtcga cgcggaactg cgaccggtag gtcttcacat aagagacgcc
     1261 ctcggcctcg atgtcgacga cgcgccaatc ctggcccgcg ccgatcatga cgaagtcgac
     1321 gtcgacctcg gtgccgtcgg tcagcgtgat gatcgtgtgc acgcgcgccg gcgaatgcgc
     1381 cgcgtcgccg tcgaacggca agacgcgcag cgtctcgggc ttgaactgga gcagcaggtc
     1441 gccgtaggtc gccacgaggt agtcgtagaa cgcggtcacg aagcggtcac gatcggccgc
     1501 tgaagcggag tcccagtgct cggccaggat cgcgcgcgag ccgcgccgca tatcgaagcg
     1561 cggcaagatc agcgtgtcga cgagcttggc gatggcctcg tgatcgtcct cgagctccgc
     1621 gcgccgcgtc gcgatcgtcg tcgcgagctc ggcggccgcg ccggcgacga tctcgttgcc
     1681 cgcgaacgtt tgcgcgagcg ccggcggcgc ggctgccgcg agcgtcgcga gcaacgcgag
     1741 gcgcgcgtag tcagtcacag gtgatcgtgt cgcccgagtg gcggcacgtc tcgggccaac
     1801 cgaaccggtc ccagtagcgg ttcaagccga tcgcgcgcac gagctcgccg aagcgcgggt
     1861 gctggcgcag ctggtgcgcc tcgcgcgcaa acacgaactc gacgttgaag ctcggccgat
     1921 cgttgacgag gcgaagcccc gcgtcgatgg cgcgctctgg ctcgccgagg tatagccacg
     1981 agccgaacaa gtatttcggc ggaatgtcgc gcgcttgctc ggcgcgcgcg agcgccgcga
     2041 cggcttgcgc gcggcgctcg ggatgcgcga gcccatcgag cagcggatcg acccaagccg
     2101 tcggcctcgc aaacatcgtc tgcacgccga tcagaagcgg ccgaaccgcg gcatagtcgc
     2161 gctcccgcag cttcagcacg atgtaggcgt cgggcgtcgg cgccatgccg agctcttcgg
     2221 cgacgagcgc gtaacgcgcc gcgtcgtcat cggcgtcgat ccacatcgaa gccaccgaaa
     2281 ggcggtgagt cacgaccggt gacaagcgat ccagctgccg cgcgcgccgc gcatgctcgg
     2341 cggaggcttc ggggcggccg acggccgcca aaaactgcga gtaccagacc agcaagtcgg
     2401 aatcgttgcg ctcatgctcg agcgcgcggc ggaacgcgac ctccgctgcg atccaatccc
     2461 aacgcgcata cgaaaccagc gcgatcacga cttgcatcga gtcgtcgatc gaggcgttgt
     2521 tcgcgaggcc cttggcaagc gtcgcgagcg ccaggtcgaa catctcgtct tgaatctcgg
     2581 ccgagtacga aggcagcagc acgtaagctt tcgcgagctc gacgtaggct tggccgtagc
     2641 gcggatcgag ctcgatcgct tgctcaaaga gcagaatgct gcgccgcagc gagtcctcgt
     2701 cgcgccggtt caagtaccgc cggccctcca gcaagcgatc gtaggcgtcg aggttcgtcg
     2761 tcggagccga cgtcgcgatc ggtgccgtcg gcagctcggt cggtaccgtg tcgcgcagtt
     2821 tcgccacgat cgtgttggcg atctcgtctt gcaacgcgaa gatgtcgccg aagacgcctt
     2881 cgtacggcgc gttccagatt cgatagccgc gccgcgcatc gacgagctgc acgtcgattt
     2941 tcacgcgttc gccgtcgcgg cggaccgtgc cttcgacgag gtgcgcgacg gcgagccgtt
     3001 ggccgatgct gcgcacgtcg tcggtcgagg cgcgcgcggc gaacgacgac gtgcgggccg
     3061 cgacctgcaa gccgtcgacg gcgctcaacc ggttcagaat ctcgtcggca agcccttcgc
     3121 cgaagtaggc ggcgtcgggc gagccgccga ccgtgaacgg cagcacggcg atcgtgttgc
     3181 gcgcaaccgc gtccgtgccg ccgctctcgg ccgtcccgcg gcccattgcc cagagcgcga
     3241 tgccggcgag cacgatcgcc gcggcaatgc cgccgccgat gacgaccgga tt
//
LOCUS       DASSNN010000459         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1464607, whole genome shotgun sequence.
ACCESSION   DASSNN010000459 DASSNN010000000
VERSION     DASSNN010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464607"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFL84_08620"
     CDS             <1..336
                     /locus_tag="VFL84_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HET7608723.1"
                     /translation="GTSTLTDITIGAQAGGQAYRQVIFFQDKAAYDKFTSGKFEFDAS
                     ASAVAVTASAQASAGTQGASSTAGAGRPNTGAAQGYVNGMQTFTMAEGGLMYAATIGG
                     QRYNFEAAK"
     gene            complement(394..>2097)
                     /locus_tag="VFL84_08625"
     CDS             complement(394..>2097)
                     /locus_tag="VFL84_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7608724.1"
                     /translation="GEKVNDLDNIGVRTQLLVTPNERTDVTFALDYTNQEPTGYAQVF
                     AGAVPTLRSNYRQFETIVADLGYAPPSRDPFDRLIDHDTPWKSGNEMGGFSANVDVDL
                     GPGTLTSTTAWRFWSWDPSNDRDYLGLPVGTLSQAPSKHEQATQEIRWAGDLSDNVSG
                     VFGFFGFDQKLNTDPVHTEQAGAALYRFLWTAPSTPAAATPELFDGYTSEIASELNTV
                     SAALYGQVDWAITDRLHLLPGLRFNYDQKDVDFARRVSGGPANPTAAERELLLGVYRP
                     QAFQTDVSDSDVSGHLTLQFAASDRINAYATLAKAFKSLGVNLGGLPLDANNNTIVEA
                     AELKPEDVTHFEIGFKASPTPGSTANVTLFDTQIEDFQTQVTNAQFGVARGYLANAEK
                     IRVRGAELDGNVNVGSRFAVRGSVAYTDAKYASFADAPPPLEGTGGPASVDASGGLLP
                     GVSKWAASVGTEYRKQGSFFRKPGEFFTGLDVYYRGDFSSSATPSQYLNVDGYSLLNL
                     RVGFRSDSGGWSGYLWARNLLDEQYFEQLLAAPAGNGAGHYGAVLGDPRVYGATLRFS
                     F"
BASE COUNT          362 a          693 c          707 g          335 t
ORIGIN      
        1 gggaccagca ccctcacgga catcacgatc ggggcgcagg ccggcggtca ggcctatcgg
       61 caagtcatct tcttccaaga caaagccgcc tacgacaaat tcacgagcgg caaattcgag
      121 ttcgacgctt cggcttccgc cgtcgcggtc acggcgagcg cacaggcctc ggcgggcacg
      181 cagggcgcgt cgtcgacggc cggcgccggc cgtccgaaca cgggtgcggc ccagggctac
      241 gtcaacggca tgcagacgtt cacgatggcc gaaggcgggc tcatgtacgc cgcgacgatc
      301 ggcgggcaac gctacaactt cgaagccgcc aaataagtgg tcgagcgagc ggcgcggttc
      361 gagccgcgcc gctcgcacgt ttccggccgc gagctagaac gagaaccgca gcgtcgcgcc
      421 atagacgcgc ggatcgccga gcaccgcgcc atagtgaccg gcgccgttgc cggcgggcgc
      481 cgcgagcagc tgctcgaagt attgctcgtc gagcagattg cgcgcccaga gataccccga
      541 ccagccgccg ctgtccgagc ggaagccgac gcgcaggttc aacaacgaat agccgtcgac
      601 gttcagatac tgcgacggcg tcgcgctcga cgagaaatcg ccgcggtaat agacgtcgag
      661 gcccgtgaag aactcgccgg gcttgcggaa gaagctgcct tgcttgcggt actcggtgcc
      721 gaccgacgcg gcccacttcg agacgcccgg cagcaagccg cccgacgcgt cgaccgaagc
      781 cggaccgccc gtgccctcga gcggcggcgg cgcatcggcg aacgacgcgt attttgcgtc
      841 cgtgtacgcg accgagccgc gcacggcgaa gcggctgccg acgttgacgt tgccgtcgag
      901 ctcggcgccg cgcacgcgaa tcttctcggc gttcgcgaga tagccgcgcg cgacgccgaa
      961 ctgcgcgttc gtgacttgcg tctggaagtc ttcgatctgc gtgtcgaaca acgtcacgtt
     1021 cgcagtcgag cccggcgtcg ggcttgcctt gaacccgatc tcgaagtgcg tgacgtcctc
     1081 cggcttgagc tcggccgcct cgacgatggt gttgttgttc gcgtcgagcg gtaggccgcc
     1141 caagttcacg ccgagcgact tgaaggcctt ggcgagggtt gcgtatgcgt tgatgcgatc
     1201 cgacgcggcg aactgcagcg tcaaatgccc ggacacgtcc gagtccgaga cgtcggtctg
     1261 aaacgcctgc ggccgataga cgccgagcaa cagctcccgc tccgcggccg tggggttggc
     1321 cggcccgccc gaaacgcgcc gcgcgaagtc gacgtccttc tggtcgtagt tgaagcgcaa
     1381 gcccggcagc aggtgcaggc gatccgtgat cgcccagtcg acctggccgt acaacgccgc
     1441 gctcacggtg ttgagctccg aagcgatctc gctcgtgtag ccgtcgaaga gctcgggcgt
     1501 cgccgccgcg ggcgtgctcg gcgccgtcca gagaaagcga tacagggccg cgccggcctg
     1561 ctccgtgtgg acgggatcgg tgttgagctt ctgatcgaag ccgaagaagc cgaagacgcc
     1621 gctcacgttg tcggagagat cgcccgccca gcgaatctcc tgcgtggctt gctcatgctt
     1681 cgaaggcgcc tgagacaacg tgccgacggg caagccgagg taatcgcggt cattcgacgg
     1741 atcccagctc caaaaccgcc atgccgtcgt cgacgtcagc gtgccgggcc ctaagtcgac
     1801 gtcgacgttg gccgagaagc cgcccatctc gttgccggac ttccacggcg tgtcgtgatc
     1861 gatgagccga tcgaacgggt cgcggctcgg cggcgcgtag cccaagtcgg cgacgatcgt
     1921 ctcgaactgc cggtaattcg agcgcagcgt cgggacggcg cctgcgaaga cttgcgcata
     1981 gccggtcggc tcttggttcg tgtaatcgag cgcgaacgtg acgtcggtcc gctcgttcgg
     2041 cgtgacgagc aactgcgtgc gcacgccgat gttgtcgaga tcgttgactt tctcgcc
//
LOCUS       DASSNN010000460         4775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_677879, whole genome shotgun sequence.
ACCESSION   DASSNN010000460 DASSNN010000000
VERSION     DASSNN010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677879"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..435
                     /locus_tag="VFL84_08630"
     CDS             19..435
                     /locus_tag="VFL84_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7608725.1"
                     /translation="MKRAKLAAGAVVVRRIDDRWHCLVLRAYRNWDFPKGLVEPGEEP
                     LEAALREVAEETGLRDLALRWGEVWCETEPYAGGKVARFYVAESASGKVTLPVNPALG
                     RPEHHEFRWLDFDAAAALLPPRLQRVLRWAQTVIDG"
     gene            complement(471..1769)
                     /locus_tag="VFL84_08635"
     CDS             complement(471..1769)
                     /locus_tag="VFL84_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608726.1"
                     /translation="MTHRALAIVSAAAFLGGVADEAAFSQERPACARGDLACWQQLHT
                     SQCAHATATLETCLVFLQRLETARKGAYSTGIALLLGETLADAASKDASPQSHERFLQ
                     RARAAYREVVKNEPFNASGYLGLAEIAATGAERVEWLRGAVRAEYQPAHMELLATALL
                     QDGGQPPELKASRELEAARVIEDAYTYESTSSERWRYSVLGLRRYTEAVERYPSAVTE
                     RAVDNVVLRVKDDIDYALLQRMLLMPESYLPYLADAFATMCEKSIAVLVTLDECMDGL
                     ELAVSTAESSFSAGTRRLLAEATLTGMRTVAGESLPRSAQARGKFIDWIDRLLATELE
                     PVDVAVDLLEARADYTPNLLERADTLQSALELLPNRGDLRLKLGATYVSLWLWPEALE
                     QLRVAKFYLPIDEQQSADTLIETADRRYQATFFPSAPSEQ"
     gene            complement(1860..3224)
                     /locus_tag="VFL84_08640"
     CDS             complement(1860..3224)
                     /locus_tag="VFL84_08640"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HET7608727.1"
                     /translation="MKRGGRRLGVLVVFAGVVAAAQAAPSVYPTGVTIYDPARTWNGY
                     TVLSPLATQAVIVIDMNGNVVKRWDDFNSSAGGPARVLPGGGVIGAKGARPPYQESLE
                     LVQRDFDGKLIWSFNRTEEIELRDVGRVWSSRQHHDWQRVDFPAGYYSPEATPAVTGS
                     NTLILTHTNHKLPAVADVNLEDDRLIEVTPDGRIVWEWTAGEHIDELGFAPDARAAIK
                     AAADVNAARNSFDWLHVNSATYLGPNRWFDAGDRRFAPTNVMISSRQASLLAIVARDG
                     KVVWRLGPDFSASEELRKIRQIIGQHHAHLIPKGLPGAGNLLVFDNGGSSGYGFAGPI
                     APNGVGAFARATSRVLEIDPVTLELVWSYTAPRFFSTNISSAQRLPNGNTLVTEGAGG
                     RMFEIVNDGTIVWEYMNPLFAGPRQSNDVYRAYRLPYDWIPQLKRPTEKAVTPPALGD
                     FSVP"
     gene            3416..>4775
                     /locus_tag="VFL84_08645"
     CDS             3416..>4775
                     /locus_tag="VFL84_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7608728.1"
                     /translation="MADDKASDQPEGTGGPTRVRGAPGIRALHLEDNPVDAELVLRRL
                     SADSLDVQCRLVADERGFRAGLADFAPQIILSDFSLRGFDGMAALEIARDMAPTTPFI
                     FVSGTIGEERAIEALKRGATDYVLKDNLRRLVPAIKSALRQAEIGLAKELAEDMLRRS
                     ESRLQDIINTSRDWIWECDREGRFTFSSPSVEQVLGYTRHEVLGRRASEYIDPADDLQ
                     LQATLADLPSGGADSSSPITLRWRHRQGKTRWLERTMVALREPDGTWRGVRGIDRDVT
                     LRIAQEARIRRLNRAQRFLSGASEAAMRIRDRNRLIREACRLAVSVGGYTRATIYMLP
                     NEVAGTPPLVCSYGDTQDDGARWLIKNRLPETSRAVSQVLATATPIVMNDLSDPANAA
                     LLGEKSALPDGPRARIALPLDIDQSVIGVVELDAQEPGVFGDAEIALLKQVAANITFA
                     LQY"
BASE COUNT          802 a         1600 c         1579 g          794 t
ORIGIN      
        1 cccccgtaga ctctctgcat gaaacgagcc aagttggcgg ccggagccgt cgtcgtgcgc
       61 cgaatcgatg accgatggca ctgcctcgtg ctgcgcgcct atcgcaactg ggacttcccg
      121 aaagggctcg tcgagcccgg cgaggagccg ctcgaggcgg cgctacgcga agtcgccgaa
      181 gaaaccggcc tgcgcgatct cgccctgcgg tggggagagg tgtggtgcga gaccgagcct
      241 tatgccggcg gcaaggtcgc gcgcttctac gtcgccgaat cggcgagcgg caaagtcacg
      301 ctgcctgtca acccggccct cggccggccc gagcatcacg agtttcgctg gctcgacttc
      361 gacgccgccg cggcgctgct gccgccgcgg ctgcagcgcg tgctgcgctg ggcgcaaacc
      421 gtgatagacg gttgacgaag gccgctagag ctgaacaagg aggagccggt ctactgctcg
      481 gacggggccg aaggaaagaa cgtggcttgg tagcgccggt ccgcggtctc gatgagcgta
      541 tcggcgctct gctgttcgtc gatcggcagg taaaacttcg cgacgcgcag ttgctcgagc
      601 gcttccggcc agagccacag gctcacgtac gtcgcgccga gcttcaagcg tagatcgcct
      661 cgattcggta agagctcgag cgccgactgc agcgtgtcgg ccctctcgag caaattcggc
      721 gtgtaatcgg cgcgggcctc gagcaggtcc accgcaacgt cgacgggctc gagctcggtc
      781 gccagtaacc gatcgatcca gtcgatgaat ttgccgcgcg cttgcgccga ccgaggcagc
      841 gattcaccgg caaccgttcg catgcccgtc agcgtcgcct cggcgagcaa tcgccgcgtg
      901 cccgcggaga acgagctctc ggccgtcgat acggcaagct cgagcccatc catgcattcg
      961 tcgagcgtga cgagcacggc gatactcttc tcgcacatcg tcgcaaacgc atcggcgaga
     1021 tacggcaggt acgactccgg catcagcaac atgcgttgca gcagcgcgta atcgatgtcg
     1081 tccttgacgc gcagcacgac gttgtcgacg gctctttcgg tgaccgcgga gggatagcgc
     1141 tccacggcct ccgtatatct gcgcagcccc aagacgctgt agcgccaccg ttcgctgctc
     1201 gtcgattcgt acgtatacgc gtcttcgatg acgcgggccg cttcgagctc ccgcgacgct
     1261 ttgagctccg gcggctggcc gccgtcctgc aacaacgccg tcgcgagcaa ttccatgtgc
     1321 gcgggctggt attcggctcg aaccgcaccg cgcaaccatt cgacgcgctc ggcgccggtc
     1381 gcggctatct cggcgaggcc caagtagccc gaggcgttga acggctcgtt cttgacgact
     1441 tctcgatagg cggcgcgggc acgctgcaga aatctctcgt gcgattgcgg cgacgcgtct
     1501 ttgcttgcgg cgtcggcgag cgtttcgccg agcagcagcg cgatgcctgt cgaataggcg
     1561 cccttgcgag ccgtctcgag ccgctgcagg aaaacgaggc aagtctcgag cgtggccgtg
     1621 gcgtgcgcgc attgcgacgt gtgcagctgc tgccagcagg ccagatcgcc gcgcgcgcag
     1681 gccgggcgct cctgcgaaaa cgctgcctcg tcggctacgc cgcccaagaa tgcggcggcg
     1741 gacacaatag cgagagcccg gtgcgtcacg gttccggcgt cccctctcga gctacgtcat
     1801 gaaggtcgaa ccaaactgac ctcgcgtacg tcgattcgtt cagccgccga tgaagacggc
     1861 taaggcacgg aaaagtcccc gagcgccggt ggtgtgacgg ccttctccgt cggccgcttc
     1921 agctgcggaa tccagtcgta cggcaatcgg tatgcgcggt agacgtcgtt cgactgccgc
     1981 ggccccgcga acaacgggtt catgtactcc cagacgatgg tgccgtcgtt gacgatttcg
     2041 aacatgcggc cgcccgcgcc ttcggtgacg agcgtgttgc cgttcggcag ccgctgcgcg
     2101 ctgctgatgt tcgtgctgaa gaaccgcggc gccgtgtacg accacacgag ctcgagcgtg
     2161 acgggatcga tctcgagcac gcgcgaggtg gcgcgagcga acgcgccgac gccgttcggc
     2221 gcgatcgggc cggcgaagcc gtagccgctc gacccgccgt tgtcgaacac gagcaggttg
     2281 ccggcgcccg gcaagccttt cgggatcaag tgcgcgtgat gctggccgat gatctggcga
     2341 atcttccgca gctcctcgct cgcgctgaag tccggcccca agcgccagac gaccttgccg
     2401 tcgcgcgcga cgatggcgag caagctcgcc tgccggctgc tgatcatgac gttggtcggc
     2461 gcgaagcgcc ggtcgccggc gtcgaaccag cggttcgggc cgagatacgt cgcggaattc
     2521 acgtgcagcc agtcgaacga gttgcgcgcc gcgttgacgt cggccgcggc cttgatcgcg
     2581 gcgcgcgcgt cgggcgcgaa gccgagctcg tcgatgtgct cgccggccgt ccattcccag
     2641 acgatgcggc cgtccggcgt gacctcgatg agccggtcgt cctcgagatt gacgtcggcc
     2701 acggccggca gcttgtgatt cgtgtgcgtg aggatcaacg tgttgctgcc cgtgacggcc
     2761 ggcgtcgcct cgggcgagta gtaccccgct ggaaaatcca cgcgctgcca atcgtgatgc
     2821 tggcgcgagg accagaccct gcccacgtca cgaagctcga tctcctcggt gcggttgaag
     2881 ctccagatca gcttgccgtc gaagtctcgc tgcacgagct cgagcgactc ttgatacggg
     2941 ggccgcgcgc ccttcgcgcc gatgacgccg ccgcccggca gcacgcgcgc cgggccgccc
     3001 gccgagctgt tgaagtcgtc ccagcgcttg acgacgttgc cgttcatgtc gatgacgatg
     3061 acggcctgcg tcgcgagcgg cgacagcacc gtgtagccgt tccacgtgcg cgcgggatcg
     3121 taaatcgtga cgccggtcgg atagaccgac ggcgccgcct gtgccgccgc aacgacgccc
     3181 gcgaagacca ccaagacgcc gagccgtctg ccgccacgct tcatcgaatc ccctccacgt
     3241 tgcacgcgcc acgaagctta gctcgcccgc gcgccgttcc cctagccgcg taatctggca
     3301 aatccatcct gacataaggc gcacggcgcc gccgcgccaa aaaacgtgat cgggttggac
     3361 taagtacgtg cgctaagcag ctatctttgg agcatatttc gtatagccct tctcaatggc
     3421 cgacgacaaa gcaagcgacc aacccgaggg aactggcggg cctacgcgcg ttcgcggcgc
     3481 gcctggaatt cgagcgctgc acctcgagga caatccggtc gacgccgagc tggtgcttcg
     3541 tcggctgtcg gccgacagcc tcgacgtgca atgtcgcctc gttgcggacg agcgcgggtt
     3601 tcgcgctggg ctcgccgact ttgcgccaca gatcatcctg tccgacttct cgttgcgcgg
     3661 cttcgacggc atggcggcgc tcgagatcgc tcgcgacatg gcgcccacga cgccgttcat
     3721 cttcgtatcc ggcacgatcg gcgaggaacg cgccatcgag gccttgaaac gcggcgcgac
     3781 ggactacgtg ctgaaggaca acttgcgccg cctcgtgccc gcgatcaaaa gcgcgttgcg
     3841 ccaggccgag attgggctcg ccaaggagct tgccgaagac atgctgcggc gcagtgagtc
     3901 gcggctccag gacatcatca acacgtcgcg cgactggatt tgggaatgcg atcgcgaggg
     3961 acgcttcacg ttctcgagcc cgagcgtcga gcaagtgctg ggctatacgc gtcacgaggt
     4021 cttgggccgg cgcgcatcgg agtacatcga cccggccgac gaccttcagc tgcaggccac
     4081 gcttgccgac ctgccctccg gtggcgccga ttcgagcagc ccgatcacgc tgcgctggcg
     4141 acaccggcag ggcaagacgc gctggctcga gcgcacgatg gtggcgctgc gcgagcccga
     4201 cggcacctgg cgcggcgtgc gcggcatcga ccgcgacgtc acgctgcgca ttgcgcagga
     4261 agcacggatc cgacggctga atcgagcgca gcgtttcttg agcggcgcca gcgaagccgc
     4321 catgcggatc cgcgaccgga accggctgat ccgcgaggca tgccgcctag cggtgagcgt
     4381 cggcggctat acacgggcca ccatttacat gctgcctaac gaggtcgccg gcacgccgcc
     4441 gctcgtgtgc tcgtacggcg acacgcagga cgacggcgcc agatggctga tcaagaaccg
     4501 gctgcccgaa acgagccgtg ccgtttctca ggtgctggcg acggccacgc cgatcgtgat
     4561 gaacgacttg agcgaccctg ccaacgccgc cttgctcggc gaaaagtcgg cgttgcccga
     4621 tgggccgcgt gcgcgcatcg ctcttcctct cgacatcgac caatccgtga tcggagtcgt
     4681 cgagctcgac gcgcaggagc cgggcgtgtt cggcgacgcc gagatcgcgc tgctgaagca
     4741 agtggcggcg aacattactt tcgcgctgca atacc
//
LOCUS       DASSNN010000461         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_585052, whole genome shotgun sequence.
ACCESSION   DASSNN010000461 DASSNN010000000
VERSION     DASSNN010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585052"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VFL84_08650"
     CDS             <1..990
                     /locus_tag="VFL84_08650"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HET7608729.1"
                     /translation="KVNADQLKEYKLADAKTTLTVTFANGSKREFLVGDAVYGGQDRY
                     VLDKQSGKAYVLSRELLSGLQVGESSLQLTDPKGFQLAKVAAVTIDAPGKSRSAVRVE
                     SGGAEGKKVKTWGEADTKKPNLTLANFIDNVTNNLRPMEYAAQVKVADLTQVLRMTYK
                     DDRGRTLGTMTLYKRAKPGEVPAGQELDPANPPPPELEYYIMTEKTRVPAVVRRDIAE
                     KMETDIDTVFASKPEEPGKSKVDPKGNPFGNGPLPAPHGANPPAPTPPAPTPTPPVPT
                     PPALKGRALAPVPAPAKTPAKAPAQAPAKAAAPTPVAPKAAAPAPAPASGSAH"
     gene            1290..>2510
                     /locus_tag="VFL84_08655"
     CDS             1290..>2510
                     /locus_tag="VFL84_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7608730.1"
                     /translation="MTRIRTHLLAVAAAMGLVGTGAMLESGLTPAHATPTVPADTSIS
                     DVAERVVESVVNISSEGTGESPAMSDPFFTDPMSPFFGGTPSRRMMQAKGSGVIVSSD
                     GRILTNSHVVNGFQTIRVTLQDGSEYAAKVVGKDTKTDLAVLQLQGKFPALKPLAFGD
                     SSKLRLGEIVLAIGDGLGVGKSVSMGIVSAKGRGGIGIEEYEDFIQTDAAINPGNSGG
                     ALVNMKGELVGINTAIASRSGGYQGIGFAIPSNMARPIMEMLARDGKVTRGYLGVDIA
                     TVTPAVLKQVKLGSSYGVVVGDVEKGTPASKAGLHAKDVITAFNGKPMRSADTLRTMI
                     AMAPPGSTVELEVVHPNGNKSTVHAKLGELPEKLHASVDDDDDDNRGNFQQHFYTMGP
                     DGQWHEVSPEQLKRH"
BASE COUNT          453 a          874 c          844 g          339 t
ORIGIN      
        1 aaggtcaacg cggaccagct gaaggagtac aagctcgccg acgcgaagac gacgctgacg
       61 gtgacgttcg cgaacggctc gaagcgcgag ttcctcgtcg gtgacgccgt ctacggcggc
      121 caggaccgct acgtgctcga caagcagtcg ggcaaggcgt acgtgctgtc gcgcgagctg
      181 ctctccggcc tccaggtcgg cgagtcgagc ctgcagctga cggatccgaa gggcttccag
      241 ctcgccaagg tcgccgccgt gacgatcgac gcgccgggca agtcgcgctc cgcggtgcgc
      301 gtggagagcg gcggcgccga gggcaagaag gtgaagacgt ggggcgaggc cgacaccaag
      361 aagccgaacc tgacgctcgc gaacttcatc gacaacgtca cgaacaacct gcggccgatg
      421 gagtacgcgg cgcaggtcaa ggtcgcggac ctgacgcagg tcctccgcat gacgtacaag
      481 gacgaccgcg gccgcacgct cggcacgatg acgctgtaca agcgcgcgaa gccgggcgag
      541 gtgcccgcgg ggcaggagct cgatccggcc aatccgccgc cgcccgagct cgagtactac
      601 atcatgacgg agaagacgcg cgtgccggcg gtcgtccgtc gcgacatcgc cgagaagatg
      661 gagaccgaca tcgacacggt gttcgcgagc aagccggagg agcccggcaa gtcgaaggtc
      721 gatccgaagg ggaacccgtt cgggaacggc ccgctgccgg ctccgcacgg cgcgaatccg
      781 ccggcgccga cgccgcctgc gccgactccg acgccgcctg tgccgacgcc gcccgcgctc
      841 aagggccgcg cgcttgcgcc agtgccggct ccggcgaaga cgccagccaa ggcgccagct
      901 caggctccgg cgaaggccgc ggctccgacg ccggtcgcgc ccaaggccgc ggcgcccgcg
      961 cccgcgcccg cgagcggcag cgcacactag aacgccgaac ggtttgcatc gccggctgcg
     1021 accgtcgcgc tccgggcgcc tcgggccgta cggccgggtc tcgcgcggcc gttaccacca
     1081 gtagcggcca cgctccgctt ccgggcgtgc ggcccgatcc tcctcggatc gctaggtctc
     1141 actcgacgct gcaaaccgtt cggcgttctc cgttcggagt tctgcggagt cagaacgcct
     1201 cacagatcgg ccagttatgc cagcggaata caggcaaccc cttgcctggt ccaacgttcg
     1261 taaccatcgg tcgtcgtaaa gaggcattca tgacccgcat ccgtacgcat ctcctcgccg
     1321 tcgccgctgc gatgggtctg gtcgggaccg gcgcaatgct ggaatccggc ctcacacccg
     1381 cgcacgcgac gcccaccgtc cccgcagata cgtcgatctc ggatgtcgcc gagcgcgtcg
     1441 tcgagagcgt cgtcaacatc agctccgaag gcacgggcga gagtcccgcg atgtcggatc
     1501 cgttcttcac cgatccgatg tcgccgttct tcggcggcac gccgagccgg cgcatgatgc
     1561 aggcgaaggg ctccggcgtg atcgtgtcga gcgacggccg catcctcacc aactctcacg
     1621 tcgtcaacgg cttccagacc atccgcgtga cgctgcaaga cggcagcgag tacgccgcga
     1681 aggtcgtcgg caaggatacg aagacagatc tcgccgtgct gcagcttcag ggcaagttcc
     1741 cggccctcaa gcctctggcg ttcggtgact cgtcgaagct gcggctcggc gagatcgtcc
     1801 tcgcgatcgg cgacggcctc ggagtcggca agagcgtctc gatgggcatc gtctcggcca
     1861 agggccgcgg cggcatcggc atcgaggagt acgaggactt catccagacg gacgcggcga
     1921 tcaaccccgg caactccggc ggcgcgctcg tcaacatgaa gggcgagctc gtcggcatca
     1981 acacggcgat cgcgtcgcgc agcggcggct accagggcat cggcttcgcg atcccgtcga
     2041 acatggcgcg gccgatcatg gagatgctcg cgcgcgacgg caaggtcacc cgcggctacc
     2101 tcggcgtcga catcgcgacg gtcacgcccg cggtgctcaa gcaggtcaag ctcggctcga
     2161 gctacggcgt cgtcgtcggt gatgtcgaga agggcacgcc ggcatcgaag gccggcctcc
     2221 acgcgaagga cgtgatcacg gccttcaacg ggaagccgat gcgctcggcc gacacgctcc
     2281 gcacgatgat cgcgatggcg cctcccggca gcacggtcga gctcgaggtc gtgcacccga
     2341 acggcaacaa gtcgaccgtg cacgccaagc tcggcgagct cccggagaag ctccacgcca
     2401 gcgtcgacga cgatgacgac gacaaccgcg ggaacttcca gcagcacttc tacacgatgg
     2461 gacccgacgg gcagtggcac gaggtctcgc cggagcagct gaagcgccat
//
LOCUS       DASSNN010000462         3138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1828312, whole genome shotgun sequence.
ACCESSION   DASSNN010000462 DASSNN010000000
VERSION     DASSNN010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828312"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2142)
                     /locus_tag="VFL84_08660"
     CDS             complement(<1..2142)
                     /locus_tag="VFL84_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7608731.1"
                     /translation="MQYFRSSSSIVAALAACLAAPIHAQEALEEIVVTALRRAESLQD
                     APATVTAFDAETIEQARVQSMRDYVQLTSNMTLMETQNNAFAFVNIRGLAQIRNVDPT
                     VAVVIDGVLSTTSLGFSQELYDVQQIEVLKGPQGALYGRNATGGAINITTKQPTTDPE
                     VYVRGGIGNGDYVQLSGVASGPLGRDDVRGRVVVGYKDADGWRDNVATGEKADPYEDL
                     TIAGKLLWDLGERTTLDLRLTYSNTESTGSQFVSNAPNFVTGFPGNAMWPGNGSAPPV
                     PGLPASITALIGDPNNTSIKHQGNEPGMDDREVVSLSAKFDWDVGAGTLTSITSLDQL
                     DHVTAAEQFAYYPFVQVQGTTAPLHPAGVLVPPAAAPLAAPGGTGQNLTFGQNRFHDS
                     ISQELRFTSPDDRALRWIGGVYFAQTDLDVMISINDDFGQGFVEQHTAPNIGGINPTV
                     SWTARFLAPLIPILGFPGASALNPNTNLSALAYNFDSNHNTAYAVFGQIEYDLTDTLE
                     LSAALRYDRDERELTIKAPDQFLPVFAFPSGREGDVREAEFDSVQPKLTLSWRPNDDW
                     TIYGTYAEGFRSGGFNLSGVAVGVATLVGAGVPGLPQGVRDSYDQEDTEGYEVGFKSS
                     LAGGALRWDAAVFDTEVSNGFTFVFVAPFTAQTTRNIKSADVSGVETSLSWLATDRLQ
                     LDLGLGFLDSEITASDWIGAGGISIIGKEMPQ"
     gene            2355..3071
                     /locus_tag="VFL84_08665"
     CDS             2355..3071
                     /locus_tag="VFL84_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608732.1"
                     /translation="MPPLRGLGLERGIAVVSCAMPGGIFWVSQSAILDVGGAATSDVL
                     LTTAHGLPADSRSVKRDCRVLVRGKRQVIAEVWHAGGDRAGPEHDWAVLLTQHVSGDV
                     HRWRPAQAPGEWLTRAVDRAAPVRLVRRYADDWQSDCRLEAKTVDPRLLLAYSCITYP
                     GQSGLPLVIALDREAEPVLIAVNIGRQSSWAGTKLDFTSVARPLDAEISATIERAAAR
                     AAQTPWRRRGSPRATRRDDG"
BASE COUNT          482 a         1034 c         1066 g          556 t
ORIGIN      
        1 ctgcggcatc tctttgccga tgatgctgat gccgccggcc ccgatccaat cgctggcagt
       61 gatctcggag tcgaggaatc ccaagccgag gtcgagctgc aagcgatccg tcgcgagcca
      121 cgagaggctc gtctcgacgc cgctgacgtc agcggacttg atgttgcgcg tcgtctgcgc
      181 cgtgaacggc gcgacgaaca cgaacgtgaa accgttcgaa acctccgtgt cgaacaccgc
      241 ggcgtcccag cgcagcgcgc ctccggcgag cgacgatttg aaaccgacct cgtagccctc
      301 cgtgtcctct tggtcatacg aatcgcgtac gccctgcggc aagcccggta cgccggcgcc
      361 gaccagcgtc gcgacgccga ccgcgacgcc gctcaagttg aagccgccgc tgcggaaccc
      421 ctccgcatac gtgccgtaaa tcgtccagtc gtcgttcggt cgccagctca gcgtgagctt
      481 cggctgcacg gaatcgaact cggcctcgcg cacgtcgccc tcgcggccgc tcgggaacgc
      541 gaacaccggc aggaactggt ccggcgcctt gatcgtgagc tcgcgctcgt cgcggtcgta
      601 acgcagtgct gcggagagct cgagcgtgtc cgtcaagtcg tactcgatct ggccgaacac
      661 cgcgtacgcc gtgttgtggt tgctgtcgaa gttgtaggcg agcgcactta agttcgtgtt
      721 cgggttcagc gccgaagccc ccgggaagcc aaggatcgga atcaacggcg ccaggaaccg
      781 cgccgtccag gacaccgtcg gattgatgcc cccgatgttc ggcgccgtgt gctgctcaac
      841 gaagccctgc ccgaagtcgt cgttgatcga gatcatcacg tcgagatcgg tctgcgcgaa
      901 gtagacgccg ccgatccagc gcagggcccg atcgtcgggt gaggtgaagc gcagctcctg
      961 gctgatcgaa tcatggaagc ggttttggcc gaacgtcagg ttctggccgg tgccgccggg
     1021 tgccgcgagt ggcgcagccg ccggaggcac caagactccg gcgggatgca gcggggcagt
     1081 cgtgccctgg acctgcacga acgggtaata cgcaaactgc tcggccgccg tgacgtggtc
     1141 gagctggtcg agcgacgtga tcgatgtcag cgtgccggcg ccgacatccc agtcgaattt
     1201 cgcggacaag ctcacgactt cgcggtcgtc cattccgggc tcgttgccct ggtgcttgat
     1261 gctcgtgttg ttcgggtcgc cgatcagcgc cgtgatcgac gccggcaggc caggcacggg
     1321 cggcgcggag ccgttgccgg gccacatcgc gttgccgggg aagcccgtca cgaagttcgg
     1381 cgcgttcgag acgaactgcg agcccgtcga ctccgtgttg ctgtacgtca gccgcaggtc
     1441 gagcgtcgtg cgctcgccca agtcccagag cagctttccg gcgatcgtga ggtcctcgta
     1501 cggatcggcc ttttcacccg tcgcgacgtt gtcgcgccag ccatccgcgt ccttgtagcc
     1561 gacgacgacg cggccgcgca cgtcgtctcg gccgagcggg ccgctcgcta cgcccgaaag
     1621 ctggacgtag tcgccgttgc cgatgccgcc gcgcacgtag acctcggggt ccgtcgtcgg
     1681 ctgcttcgtc gtgatgttga tcgcgccgcc cgtggcgttg cgtccgtaga gggcgccctg
     1741 cgggcctttg agcacttcga tctgctggac gtcgtagagc tcttgcgaga acccgaggct
     1801 cgtcgtcgac aacacgccgt cgatgacgac cgcgaccgtc ggatcgacat tgcggatctg
     1861 agccaggccg cggatgttga cgaacgcgaa cgcgttgttc tgcgtttcca tcagcgtcat
     1921 gttcgaagtg agctgcacgt agtcgcgcat gctctggacg cgtgcttgct cgatggtctc
     1981 cgcgtcgaac gcggtgaccg tagccggcgc atcctgcaag ctttcggcgc ggcgaagggc
     2041 cgttacgacg atctcttcga gcgcctcttg agcgtggatc ggtgccgcga gacaggcggc
     2101 gagcgcggcg acgattgacg acgacgagcg gaagtactgc atggtgtgtc tcccggatgg
     2161 tgttgtcgca ttgccaacaa taccaggtcc ggggaggaac cggtccgtcc aacccgttcg
     2221 gcgatctgcg cggctggttt tgccggccgc agccgcggta tcgtggccgc atgcgcgccg
     2281 tatggctcgt ggcctgtttt gtccccgtgg cttgtttgct ccccggggct gccgagacaa
     2341 gcgagattac cgccttgccg ccgctgcgcg gcttgggtct cgagcgcggc atcgccgtcg
     2401 taagttgcgc gatgcccggc gggattttct gggtaagcca aagcgcgatt ctggatgtcg
     2461 gcggcgccgc gacctcggac gttctgctga cgaccgcgca cggcttgccg gctgactcgc
     2521 ggtccgtcaa gcgcgattgc cgcgtgctcg tgcgcggcaa acgacaggtc atcgccgaag
     2581 tgtggcacgc cggcggcgac cgagcgggcc ccgagcacga ttgggcggtc ctgttgacgc
     2641 agcacgtcag cggcgacgtg caccgatggc gccccgcgca ggcccccggc gagtggctga
     2701 ccagggccgt cgatcgcgcc gcgccggtcc ggctcgttcg ccgttacgcg gacgactggc
     2761 aaagcgattg ccgcctcgag gcgaagaccg tcgatccgcg gctgctcctc gcatattcct
     2821 gcattaccta tcccggtcag tccgggttgc cgctcgtgat cgcgctcgac cgcgaggcag
     2881 agcccgttct gatcgccgta aacatcggca ggcagagcag ctgggccggc accaagctcg
     2941 acttcacgag cgttgcgcgg ccgctcgacg cagagatcag cgcgacgatc gagagggccg
     3001 ccgcgcgcgc agcgcagacg ccgtggcggc ggcgcggctc gccgcgtgcg acgaggcgcg
     3061 acgatggcta aggccccgtc gagttgccct ggcggctcgt gttagtgcaa tactcacgcg
     3121 tccctcgaag ggagctca
//
LOCUS       DASSNN010000463         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1212119, whole genome shotgun sequence.
ACCESSION   DASSNN010000463 DASSNN010000000
VERSION     DASSNN010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212119"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..773)
                     /locus_tag="VFL84_08670"
     CDS             complement(114..773)
                     /locus_tag="VFL84_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7608733.1"
                     /translation="MKRILRRSLVTAAACIAALAAASCGPAKPHNTGVYLLLDTSGTY
                     REELDKAKQIINFTLARLDGLDTFAVARIDTGSFSEQDIMAKVTLDDRPSAANQQKRE
                     FAQQINKFLDETPSSPYTDITGGLLQAVEFLNEKQPGRKTIFIFSDLKEDLQAGYVRD
                     IEVPVDGFEIVALNVTKLRSDNVDPREYMTRLEEWKERVEAEGGTWRVINDLDRLETL
                     L"
     gene            complement(770..2200)
                     /locus_tag="VFL84_08675"
     CDS             complement(770..2200)
                     /locus_tag="VFL84_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608734.1"
                     /translation="MKFESLLGITPYPGLSAVILAVLAMAVLYLARGSAHHVLLTVCQ
                     ALHDILNMAVQAITASEKRLVQRNHEVLLAAGREAKERVVEREFVRIGDSVHRDLAGY
                     PGLQRALSDAIVHIEEDHQRAAEVPPEPTAWVKAVEVVAKLDRQELSVGPLLSDIHAS
                     LVKAQAAALKEYRDASAKRHSLLQRMMPHWRRIQHTMDRVGKSVDSLLERSRSIDRHL
                     AEYREILQGTDHSIQVLSSSSLVQFFVSALVLCVAVGGATINFSLIARPMAEMVGGTA
                     FIGNFRMADIAALVIIMVEISMGLFLMESMRITRLFPVIGALPDKLRVRMMAVTFTIL
                     LMLASVEAGLAYMREILMQDELATAAILRGGAETAQSPFMWITTAAQMGMGFTLPFAL
                     VFVAIPLETFVQSLRTVLGLIGIGALRFLRLVLQLLGSAFVHAGTALIRIYDIPIFMP
                     LFLEHLWRGPSSKRPVFGKGKAEVGS"
     regulatory      complement(2202..2284)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2391..2990)
                     /locus_tag="VFL84_08680"
     CDS             complement(2391..2990)
                     /locus_tag="VFL84_08680"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7608735.1"
                     /translation="MSLRKTLLLVAIGACYVSASMPATLKKVDVSRREDRYRVAADTH
                     LDASPEAIYKVLMDFDADHYQRISEIYKESSYLPPDRDGTPLVYTRVEGCLLRYCRSM
                     SRVERLEVVTPQFIRSRTVPERSDFKYSLSEWTFEPEGAGTRVTYLMEMEPDFWLPPF
                     VGPWFLKRTLVKGAPAAIDRIEQLAQQEERAATSAAAAR"
BASE COUNT          552 a         1021 c          984 g          555 t
ORIGIN      
        1 cggcaacccc tcggttgccg gccttttcct ctaaaagggg acagatctat tttccgcgcg
       61 cagcggctcg cacggccacg ctgcaaaatc gcggaaaata gatctgtccc cttttagagg
      121 agcgtctcga ggcggtcgag gtcgttgatg acgcgccagg tgccgccttc ggcctcgacg
      181 cgctccttcc attcctcgag ccgcgtcatg tactcgcgcg ggtcgacgtt gtcgctgcgc
      241 agcttcgtga cgttcagcgc gacgatctcg aagccgtcga cgggcacctc gatgtcgcgc
      301 acgtagccgg cctgcaagtc ctctttgagg tccgagaaga tgaagatcgt cttgcggccc
      361 ggctgcttct cgttcaagaa ctcgacggcc tgcagcagcc cgcccgtgat gtccgtgtac
      421 ggcgaggacg gcgtctcgtc gagaaacttg ttgatctgct gcgcgaactc gcgcttctgc
      481 tgattcgccg cgctcggccg gtcgtcgagc gtcaccttgg ccatgatgtc ctgctcgctg
      541 aagctgcccg tgtcgatgcg cgcgacggcg aacgtatcga gcccgtcgag ccgcgccagc
      601 gtgaaattga tgatctgctt ggccttgtcg agctcctcgc ggtacgtgcc cgacgtgtcg
      661 agcagcagat agacaccggt gttgtgcggc ttcgccgggc cgcacgaggc ggccgcgagt
      721 gccgcgatgc aggccgccgc cgtcaccagc gatcgccgca gtatccgctt catgacccca
      781 cctccgcttt gccttttccg aaaaccgggc gcttcgagct gggaccgcgc cacagatgct
      841 cgaggaacaa cggcatgaag atcggaatat cgtagatgcg tatgagggcc gttcccgcat
      901 gcacgaacgc gctgcctaaa agctgcagca cgagccgcag aaaacgcagc gcgccgatgc
      961 cgatcaaccc gagcacggtt cgcagcgact gcacgaaggt ctcgagcgga atcgcgacga
     1021 acacgagcgc gaacggcagc gtgaagccca tgcccatctg cgccgccgtc gtgatccaca
     1081 tgaacgggct ctgcgcggtc tccgcgccac cgcgcaggat cgccgccgtc gcgagctcgt
     1141 cctgcatgag gatctcgcgc atgtacgcga gaccggcttc gacggacgcg agcatcaaga
     1201 gaatcgtgaa cgtgaccgcc atcatccgca cgcgcagctt gtccggcagc gcgccgatga
     1261 cggggaagag ccgcgtgatg cgcatcgatt ccatcaggaa gagacccatc gagatctcga
     1321 ccatgatgat gacgagcgcc gcgatgtcgg ccatgcggaa attgcctatg aacgccgtgc
     1381 cgccgaccat ctccgccatc ggccgcgcga tcaacgagaa gttgatcgtc gcgccgccca
     1441 cggcgacgca gagcacgagc gccgagacga agaactgcac gagcgacgac gacgacagca
     1501 cctggatcga gtgatcggtg ccctggagaa tctcgcggta ctcggcgaga tggcggtcga
     1561 tcgagcgcga ccgctcgagc aggctgtcca cgctcttgcc gacgcggtcc atcgtgtgct
     1621 ggatgcgccg ccagtgcggc atcatgcgct ggaggagcga gtgccgcttt gcgctcgcgt
     1681 cgcgatattc cttcagcgca gccgcctgcg ctttgaccag cgatgcgtga atgtcgctga
     1741 gtagcggccc tacggacagc tcctggcggt cgagcttcgc gacgacctcg acggccttga
     1801 cccacgccgt cggctcgggc ggcacttcgg ccgcgcgctg gtggtcttcc tcgatgtgaa
     1861 cgatcgcatc gctgagcgcc ctttgtagac cgggatagcc cgcgagatcg cgatggacgc
     1921 tgtcgccgat gcgcacgaac tcgcgctcga cgacgcgctc cttggcttcg cgtccggccg
     1981 ccagcaagac ctcgtgattg cgctggacga gcctcttctc gctcgccgtg atcgcttgca
     2041 ccgccatgtt caggatgtcg tgcagcgcct gacaaaccgt cagcagcacg tggtgcgccg
     2101 aacctcgcgc gagatagagg acggccattg cgaggacggc caaaataacg gccgacaatc
     2161 cgggatacgg agtgatcccg agaagactct cgaacttcat gactggcaac cccgctgtcg
     2221 taggtttccc gttagaccgg aagctttgcg acccttcctt tcggaaggtg tgccccttgc
     2281 agtgcagcga agcatcggtc gtcggcggga ccgttgccaa ccgtcttgtc ccgcaaccgt
     2341 gatgcagttc ccgcgcgcgg tccttatgtc aacgccggcg gcgtgaagcg ctagcgcgcc
     2401 gccgccgcgc tcgttgcggc gcgctcctct tgctgcgcga gctgctcgat ccgatcgatc
     2461 gccgcgggcg ctcccttgac gagcgttcgc ttcaaaaacc atggcccgac gaacggcggc
     2521 agccagaagt ccggctccat ctccatcaga tacgtgacgc gcgtgcccgc gccttcgggc
     2581 tcaaacgtcc actcggacag cgagtatttg aagtcgctgc gttcgggtac ggtgcgggac
     2641 cgaataaact gcggcgtgac gacctcgagc cgctcgacgc ggctcatgct cctgcaataa
     2701 cgcaacaggc agccctccac gcgcgtatag acgagcggag tgccgtcgcg gtccggcggc
     2761 aggtagctgc tctccttgta gatctcggag atgcgctggt agtgatcggc gtcgaaatcc
     2821 atcaacacct tatagatggc ttcgggcgag gcatcgaggt gcgtgtccgc ggcgacgcga
     2881 taacggtctt cgcggcgcga gacgtcgacc ttcttgagcg ttgcaggcat gctcgcggat
     2941 acgtagcagg cgccgatggc aacgaggagc aacgttttgc gcaaactcat ctgcggtcgg
     3001 cgacgcgtgg gctttgggag cccgaaacta tacgctgccg ctgcgttggc cgcgacgctc
     3061 gccgcttctc cgctgcacgc ccagcaagac gagggccccg ctaacgaacg ag
//
LOCUS       DASSNN010000464         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_314641, whole genome shotgun sequence.
ACCESSION   DASSNN010000464 DASSNN010000000
VERSION     DASSNN010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314641"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1364)
                     /locus_tag="VFL84_08685"
     CDS             complement(312..1364)
                     /locus_tag="VFL84_08685"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET7608736.1"
                     /translation="MPALLFAQEPETVEGEAFVPITPIERLNPSYPTRALQHGNSGWV
                     MLSFVVSETGEVEEAMIEDSSGAVFEDVALDAVMKWRYTPATVNGKPVAQSMVKTRIL
                     FDLEGMAKGVTREFQGSYRKIRDLLDTGDYAAAKPLLDELETEGRQNLYEDALFWALK
                     YGYLEAVQSGDAAAKRRALELALGHQDDSLLPADTFIAAAQRLYVLQVQAVDFSAARA
                     TFERLRDSEYAKRSSQHARAVAELTPAYEQIERVIAGRALLKVKGEIGDHAYWVHDLM
                     RRAFSMADVNGEIAALDVRCERGTRRYNMYPVDDVWVVPESWGKCGLYVNGERGTTFT
                     LVEHPAGTPATPLARE"
     gene            complement(1431..>2343)
                     /locus_tag="VFL84_08690"
     CDS             complement(1431..>2343)
                     /locus_tag="VFL84_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HET7608737.1"
                     /translation="GEWYSPTQPIPTKPPAYDRQGVTENDLIDFTPALRREALDIVTW
                     HKMGPLFTPPVASNINGPLGTLMAPSQDGGTNWQGGSFDPETGILYVASASTVVSKAV
                     VPPYPGQSEVAYIEGNAVTGPRTTGGSGSAAGGGRSEFQPAERRVLTPDDPSRGPPKL
                     ALSVQGLPLLKPPYGRISAIDMKRGEILWQIAHGETPDDIKENPALKGLTIPRTGQAG
                     SPGTLVTKTLLISGERLNTTTPEGLGAMLRAYDKASGREVGAVYMPAPQSGSPMTYLQ
                     NGEQYLVVAISGAQYAGELLAFRLPRR"
BASE COUNT          373 a          795 c          742 g          433 t
ORIGIN      
        1 agtgattgaa cggcactcga cttcagtgtc gtgtccggac cgacgctcca cgcgcccgga
       61 cgctcgtgat aaagaatggt gaaatcgtcc gcgtatgctg ccgccacgtg cagcagcgcg
      121 accgccgcga gcatgcagcg cttagcgatc caggatcccc ctccccgtaa tcccatatcg
      181 atggcaacga cgtagcggca cggcaattag tgcgcgagcg gcgcatcgag tatcgtccgc
      241 cggctctatg gcggcaagga aaaccagacg gcgatgccaa cttctgtatg acacttatac
      301 aactcgctac gtcattcgcg tgccaaaggc gtcgcaggcg tgccggcggg gtgttccacc
      361 agcgtgaacg tcgtaccgcg ctcgccgttg acatagaggc cgcatttgcc ccagctctcg
      421 ggcactaccc agacatcgtc gaccggatac atgttgtagc gacgcgtgcc gcgctcgcag
      481 cgcacatcga gtgcagcgat ctcgccgttc acatcggcca tcgagaatgc gcggcgcatg
      541 aggtcgtgga cccaataggc gtggtcgccg atctcgcctt tgaccttcag cagcgcccgc
      601 cccgcgatca cgcgctcgat ttgctcgtac gccggcgtca actccgcaac ggcgcgcgcg
      661 tgctggctgg acctcttcgc atactcggag tctcgcaggc gttcgaaggt ggcgcgggcc
      721 gcgctgaaat cgaccgcctg cacctgtagc acgtacaagc gctgcgccgc ggcgatgaag
      781 gtatccgcgg gcaacagcga gtcgtcctgg tgaccgagcg cgagctccaa cgcccttcgc
      841 ttcgcggccg catctcccga ttgcacggct tcgaggtagc catacttgag cgcccagaac
      901 agcgcgtcct cgtagagatt ctgtcgcccc tcagtctcga gctcgtcgag gagcggcttg
      961 gcggcagcgt agtcgcccgt atcgagcaag tccctgattt tgcggtacga gccttggaac
     1021 tcgcgcgtca cgcccttcgc catgccctcg aggtcgaaca agattcgcgt cttgaccatg
     1081 ctctgcgcga ctggcttacc gttgaccgtg gcgggcgtgt agcgccactt catgaccgca
     1141 tccaatgcca catcctcgaa taccgcgccg ctcgagtctt cgatcatggc ctcctcgacc
     1201 tcaccggtct cggatacgac gaagctcagc atgacccagc ccgagttgcc gtgctgcaag
     1261 gctcgcgtcg ggtagctcgg gttcaagcgt tcaatcggcg tgatcggcac gaacgcttcg
     1321 ccttcgaccg tttccggctc ctgtgcgaaa aggagcgccg gcatggacgc tgacagcaac
     1381 gcgagtgcaa cacgcatcac gactcctttc tctgtcggca gcgccgccgt tcagcgccgc
     1441 ggcagcctga acgccagcag ctcgcccgcg tactgcgcgc cgctgatcgc gacgacgaga
     1501 tactgctcgc cgttttgcag gtacgtcatc ggcgagccgc tctgcggcgc cggcatgtag
     1561 accgcgccga cctcgcggcc gctcgctttg tcgtaggcgc gcagcattgc gccgagccct
     1621 tccggcgtcg tcgtgttcaa gcgctcgccg gaaatgagca gcgtcttcgt cacgagcgtg
     1681 cccggcgagc cggcctggcc cgtgcgcgga atcgtcagtc ccttcagcgc ggggttctct
     1741 ttgatgtcgt cgggcgtctc gccgtgcgcg atctgccaga ggatctcgcc gcgcttcatg
     1801 tcgatcgcgc tgatgcggcc gtatggcggc ttcaacagcg gtagaccttg cacggagagc
     1861 gcgagcttcg gcggtccacg cgacgggtcg tcgggcgtca gcacgcgccg ttcggccggc
     1921 tggaactcgc tgcgccctcc tccggccgcc gagcccgagc cgcccgtcgt gcgcggtccg
     1981 gtcacggcgt tgccttcgat gtaggcgacc tcggattgcc cgggatacgg cggcacgacg
     2041 gccttgctga cgaccgtcga ggccgacgcg acgtacagga ttcccgtctc gggatcgaag
     2101 gagccgccct gccaattcgt gccgccgtcc tggctcggcg ccatcagcgt gccgagcggc
     2161 ccgttgatgt tgctggcgac gggcggagtg aacagcgggc ccatcttgtg ccacgtgacg
     2221 atgtcgagcg cctcgcggcg caacgccggc gtgaagtcga tgagatcgtt ctcggtgacg
     2281 ccttggcggt cgtacgcggg cggtttcgtc ggtatcggct gcgtcggtga ataccactcg
     2341 ccg
//
LOCUS       DASSNN010000465         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_822598, whole genome shotgun sequence.
ACCESSION   DASSNN010000465 DASSNN010000000
VERSION     DASSNN010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822598"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1003)
                     /gene="htpX"
                     /locus_tag="VFL84_08695"
     CDS             complement(122..1003)
                     /gene="htpX"
                     /locus_tag="VFL84_08695"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HET7608738.1"
                     /translation="MKRIVLFAAANVAVLAVLYVVLNVLGVERALARGGSQLDLGELL
                     VFSAIFGFLGSFISLALSKWMAKMATRAHVIDSPRNAAESWLKDTVYRLADATKIGRP
                     EVAIYDSPDVNAFATGASRNHALVAVSTGLLSRLSEREIEAVLAHEVSHIANGDMVTM
                     TLLQGVLNTFVIFFSRVIGFVVDRLLLRNERGTGPGFFLATIVAQILLGILATLIVMW
                     FSRHREFRADAGGAKLAGRDSMIRALESLRRSTQPAQLPEPLRAFGISNGRVGLGRLF
                     MSHPPLEERIAALQRAS"
     gene            1139..2071
                     /locus_tag="VFL84_08700"
     CDS             1139..2071
                     /locus_tag="VFL84_08700"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HET7608739.1"
                     /translation="MPSDNFWTTKFAALRYAVAWLGYGWMRLVVMLPFGAQRALGKAL
                     GRLAYVLLGKRREIAARNIDACLRELPAEKRARILKLHFESLGISLVEMAMGWFGPLD
                     EVRGYVDVEGAEHLTAALAQGRGVILFSAHFTPFELSWPALKALCPRLAGMYKWQRNP
                     VMNRIMQLGRGRSYDVQIAKDNVREMLRELRANAVFWYASDQSYGGKGSVLLPFFGEP
                     AMTNTAIGRIAKASGAAVLPQYCRRLENGRYLMTIGAPLRGVPSGDEIADTRALTSKL
                     EEYIRTCPEQYWWIHQRFKGRPAPLPNLYAASAS"
     gene            2096..>2867
                     /locus_tag="VFL84_08705"
     CDS             2096..>2867
                     /locus_tag="VFL84_08705"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HET7608740.1"
                     /translation="MKFPEELSLAPFWSPRYWPMWALLVLMRGAAKLPPRWQLKVGRA
                     FGRLLRRVKARQRRVARRNIELCFPALSAAEKRGLLERHFEAVGMSFVEMGVGWFTPI
                     ERLLRRVEIHGREHLERALANGRGALLVGGHFTALEIGFAVLEALTPRVATMYRTQRN
                     PMMDVMIRRGRYRFAKTQIPRDNVRALLRTLRDGNAVGYLPDQTYLGNQSELLPFFGE
                     PAVTNTATSKLAAISGAAVLTYFFRRLPSGDEIADTRAL"
BASE COUNT          510 a          939 c          953 g          465 t
ORIGIN      
        1 taccttgagc aaacttgcca tgccgagaac ggctaaaaat ccacgggatc cgatcatcaa
       61 ctagactcta gatcaacggc gcgaaaaata gcaacgcacc agttccgcgg ccgctcgcgc
      121 tttagcttgc tcgctgcagc gcggcgatgc gctcctcgag cggcgggtgc gacatgaaca
      181 gccgtcccag gccgacgcgc ccgttcgaga tgccgaacgc acgcagcggc tccggcaact
      241 gcgcaggctg ggtcgagcga cgcaggctct cgagggcgcg gatcatggaa tcgcgccccg
      301 ccagcttcgc gccgcccgcg tccgcgcgga actcgcgatg ccgcgagaac cacatcacga
      361 tcagcgtggc gagtatgccg agcaggatct gcgcgacgat ggtcgcgagg aaaaaacccg
      421 gccccgtgcc gcgctcgttg cgcagcagca ggcggtcgac gacgaacccg atcacgcgcg
      481 agaagaagat cacgaacgtg ttcaggacgc cttgcagcag agtcatcgtc accatgtcgc
      541 cgttcgcgat gtggctgacc tcgtgcgcta gcacggcttc gatctcgcgc tcggaaaggc
      601 gagacaagag gcccgtgctg acggcgacga gagcgtgatt acgcgacgcg ccggtcgcga
      661 aagcattgac gtcgggcgaa tcgtagatcg cgacctcggg ccggccgatt ttggtcgcgt
      721 ccgcgaggcg gtacaccgtg tccttgagcc agctctcggc cgcattgcgc gggctgtcga
      781 tgacgtgcgc gcgcgtcgcc atcttggcca tccacttcga cagcgcgagc gagatgaacg
      841 agcccaagaa gccgaagatc gcggagaaca cgagcaactc gccgagatcg agctgcgagc
      901 cgccgcgcgc cagcgcccgc tcgacgccga gcacgttcag cacgacgtag aggacggcga
      961 gaaccgcgac gttcgccgcc gcgaacaaga cgatgcgctt catcgatttc tccggagcct
     1021 ccgtacgaga ttgacggcgc gagatatggc gccgccgttc ggggatatca agctgccgcc
     1081 ggcgcacatg ctgctaagat cacctcaagt tttatggcgc gaaagacgcg tcccgagcat
     1141 gccctctgat aatttctgga ccacgaagtt cgctgcgctg cgctacgccg tcgcctggct
     1201 cggctacggc tggatgcggc tcgtcgtcat gctgcccttt ggcgcgcagc gcgcgctcgg
     1261 caaagcgctc ggccgcctcg cgtatgtcct gctcggcaag cgccgcgaga tcgcggcacg
     1321 caacatcgac gcctgcctgc gcgagctgcc ggccgaaaag cgtgcgcgaa tcctgaagct
     1381 gcacttcgaa tcgttgggga tctcgctcgt cgagatggcg atgggctggt tcgggccgct
     1441 cgacgaggtg cgcggatacg tggacgtcga aggcgccgag catctgaccg ccgcgctcgc
     1501 gcaaggcagg ggcgtcattc tgttctcggc gcacttcacg ccgttcgagc tctcttggcc
     1561 ggcgctgaag gcgctctgcc cgaggctcgc cggcatgtac aaatggcagc gcaatcccgt
     1621 catgaaccgc atcatgcagc tcggacgcgg acgctcctac gacgtccaga tcgcgaagga
     1681 caacgtccgc gagatgctgc gcgagctgcg cgcgaacgcc gtcttctggt acgcgtcgga
     1741 ccaaagctac ggcggcaagg gcagcgttct cctgccgttc ttcggcgagc ccgcaatgac
     1801 gaacactgcg atcggccgaa ttgcgaaggc gagcggcgcc gccgttctgc cccagtattg
     1861 ccgcaggctc gagaacggcc gttatctgat gacgatcggc gcgccgcttc gcggcgtacc
     1921 gagcggcgac gagatcgcgg acacgcgcgc gctgacgagc aagctcgagg aatacattcg
     1981 tacgtgcccg gagcagtact ggtggatcca ccaacgcttc aagggccgcc cggcaccgct
     2041 tccgaatctc tatgccgcat cggcgtcgtg aaggcgcgtt cgcggaatga gacgcgtgaa
     2101 attccctgag gaattgtcgc tggcgccatt ctggagcccc cgttactggc cgatgtgggc
     2161 actgctcgtc ctcatgcgag gcgccgcgaa gctcccgcct cggtggcagc tcaaagttgg
     2221 gcgcgcgttc ggccgcttgt tgcggcgagt aaaggcgcgg cagcggcgcg tcgcgcggcg
     2281 caacatcgag ctctgctttc cggctttgag cgcggccgag aagcgcggct tgctcgagcg
     2341 gcacttcgaa gccgtgggca tgtcgttcgt cgagatgggc gtcgggtggt tcacgccgat
     2401 cgaacgcctg ctgcggcgag tcgagatcca tggccgcgag catctcgaac gcgcgctcgc
     2461 gaacggccgc ggtgcgctgc tggtcggcgg gcacttcacg gctctcgaga tcggcttcgc
     2521 cgtgctcgag gcgctgacgc cgcgcgtcgc cacgatgtat cgaacccagc gcaatccgat
     2581 gatggacgtg atgattcgcc gcgggcgcta tcggttcgcg aagacgcaga tcccgcgcga
     2641 caacgtgcgc gcgctgcttc gcacgctgcg cgacggcaac gcggtcggct acctgccgga
     2701 tcagacgtat ctcggcaacc agagcgaatt gctgccgttc ttcggcgagc ccgccgtgac
     2761 gaatacggcg acgagcaagc tagcggcgat cagcggcgcc gcggtgctga cgtacttctt
     2821 tcgccggctg ccgagcggcg acgagatcgc ggacacgcgc gcgctga
//
LOCUS       DASSNN010000466         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_211003, whole genome shotgun sequence.
ACCESSION   DASSNN010000466 DASSNN010000000
VERSION     DASSNN010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211003"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFL84_08710"
     CDS             complement(<1..263)
                     /locus_tag="VFL84_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HET7608741.1"
                     /translation="MQLIPAIDLRDGRCVRLLQGDFERETRYSVDPVQLAEHYRELGA
                     RALHVVDLDGAKRGVPMNLPVIRRMRAAAGVSVQLGGGIRARA"
     gene            complement(277..879)
                     /gene="hisH"
                     /locus_tag="VFL84_08715"
     CDS             complement(277..879)
                     /gene="hisH"
                     /locus_tag="VFL84_08715"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HET7608742.1"
                     /translation="MKPNAVIIPSCGSNLASLKFALERLGADVPLTEDAARIRAASHV
                     ILPGVGAAAPGMERLAAAGLVDLIPTLKQPVLGICLGMQLLFARSEEEETPCLGVIDA
                     QIRRLPASPDLPVPQMGWNELAPLGESALLAGVPKGAYAYFVHSYAAPTGPYTRAVTN
                     YGLPFSAVVEQGNFFGTQFHPERSSRTGAQILDNFLKIDV"
     gene            complement(876..1934)
                     /gene="hisB"
                     /locus_tag="VFL84_08720"
     CDS             complement(876..1934)
                     /gene="hisB"
                     /locus_tag="VFL84_08720"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HET7608743.1"
                     /translation="MRRVLFVDRDGTLVAERKSLAEPVEQPLVAGVIPALLKLKSAGY
                     QFVIVTNQPGLGGAGHPRAAFEQADEKLRALFASQGIRFLGNFICPHLPEARCACRKP
                     AVGLVRDFLSLGPLDRERSAVVGDRDTDVELALNLGLRGFKLGAPGSETWEALAHLLA
                     DRPRIATVQRRTKETDIRVTVDLDSEAEPSAKTGLGFFDHMLEQLGKHGGFKLDVQCT
                     GDLHIDEHHTVEDVALTLGQALKEALGDKRGIQRYGFMLPMDEADAEVSVDLGGRPYL
                     VFEAKFARDRVGDLPTELVPHFFRSLAETLGAAIHVRVRGENTHHMVEVCFKAVARSL
                     RQAFKRDGDELPTTKGRL"
     gene            complement(1927..>2788)
                     /gene="hisC"
                     /locus_tag="VFL84_08725"
     CDS             complement(1927..>2788)
                     /gene="hisC"
                     /locus_tag="VFL84_08725"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET7608744.1"
                     /translation="QILVTRGSSEAIDVLVRGFCAAGRDKILVCPPTFDMYRLYAGIQ
                     NAGVVRVPLKAEQDFALDTDKILAAVDASVKIVFICSPNNPTGQSMARADIERICRAA
                     AGRALVVIDEAYHEFATGGDFLELRARYEHVVLLRTLSKFVSLAGVRCGLIVAAPELI
                     EFAQVVLPPYTFPTPSIELVQQALSQDSLKVSEERVGVLKRERARLSAALRDVPEVVQ
                     VYPSDANFILVRTRDGQAFRETARRADILVRTFDDPLLENCVRITVGRPSDNDLLLQA
                     VSQAERKRHA"
BASE COUNT          458 a          928 c          973 g          429 t
ORIGIN      
        1 ctcgcgcggg cgcggatgcc gccgccgagc tgcacgctca ctccggcagc ggcgcgcatg
       61 cggcggatca ccggcaagtt catcggcacg ccacgcttcg cgccgtcgag atcgacgacg
      121 tgcagcgcgc gcgcgccgag ctcgcggtag tgctcggcga gctgcaccgg gtcgacggag
      181 tagcgcgtct cgcgctcgaa gtcgccttgg aggaggcgga cgcagcgccc atcgcgcagg
      241 tcgatggcgg gaatcagttg catttatttc aacgtgttac acgtcaatct ttaggaaatt
      301 atcgaggatc tgcgcgcccg tgcgcgacga gcgctcggga tgaaattgcg tgccgaaaaa
      361 gttgccttgc tcgacgacgg ccgagaacgg cagcccatag ttcgtcacgg ctcgcgtgta
      421 ggggcccgtc ggcgccgcgt agctgtgaac gaagtaggcg tacgcgcctt tcggcacgcc
      481 ggcgagcagc gccgactcgc cgagcggcgc gagctcgttc cagcccatct gcgggaccgg
      541 caggtcgggg ctcgcgggca gacgccggat ctgcgcgtcg atgacgccga ggcacggcgt
      601 ttcttcctcc tcggagcgcg caaacaggag ctgcatgccg agacaaatgc cgagcacggg
      661 ctgcttcagc gtcggaatca agtcgacgag ccccgcggcc gcgaggcgct ccatgccggg
      721 tgccgcggcg ccgacgccgg gaagaatgac gtggctcgcc gcgcggatgc gcgcagcgtc
      781 ttccgtcaac ggcacgtcgg cgccgagccg ctcgagcgcg aacttgagcg acgcgagatt
      841 cgagccgcac gacgggatga tcacggcgtt cggcttcata gccggccttt cgtcgtcggc
      901 agctcgtcgc cgtcgcgctt gaaggcctgg cgcagcgacc gcgcgacggc cttgaagcac
      961 acctcgacca tgtggtgcgt gttctcgccg cgcacgcgaa catggatcgc cgcgccgagc
     1021 gtctcggcga gcgagcggaa gaagtgcggc acgagctcgg tcggcaaatc gccgactcgg
     1081 tcgcgcgcga acttggcttc gaagacgaga tacggtcgtc cgccgagatc gacggacact
     1141 tcggcgtccg cttcgtccat cggcagcatg aagccgtagc gttgaatgcc gcgcttgtcg
     1201 ccgagcgctt ccttgagcgc ttgtcccagc gtcagcgcga cgtcctcgac agtgtggtgc
     1261 tcgtcgatgt gcagatcgcc cgtgcactgc acgtcgagct tgaagccgcc gtgcttgccg
     1321 agctgctcga gcatgtggtc gaagaagccg agcccggtct tcgcgctcgg ctcggcctcg
     1381 ctgtcgagat cgaccgtcac gcgaatgtcg gtctccttcg tgcgccgctg caccgtcgca
     1441 atgcgcggcc gatcggcgag caggtgggca agcgcttccc acgtctcgga gcccggcgcg
     1501 ccgagcttga agccgcgtaa ccccaggttg agcgcgagct cgacgtcggt atcgcggtcg
     1561 ccgacgacgg ccgagcgctc gcggtcgagc gggcccaagc tcaagaaatc gcgcacgagg
     1621 ccgacggcgg gcttgcggca agcgcaccgc gcctccggca gatgcggaca aatgaagttg
     1681 ccgagaaagc gaatcccttg cgacgcgaac agcgcgcgca gcttctcgtc ggcttgctcg
     1741 aacgccgcgc gcgggtggcc ggcgccgccg agccccggct ggttcgtcac gatgacgaat
     1801 tgatagccgg ccgacttcaa cttcagcagc gcgggaatca cgccggccac gagcggctgc
     1861 tcgaccggct ccgcgaggct cttgcgctcg gcaaccagcg tgccgtcgcg atcgacgaac
     1921 agcactctac gcatgacgtt tgcgctccgc ttgggacacg gcttgcagca gcagatcgtt
     1981 gtcggatggg cgcccgaccg tgatgcgcac gcagttttcg agcagcggat cgtcgaacgt
     2041 gcgcacgagg atgtcggcgc ggcgcgccgt ctcgcggaac gcctggccgt cgcgcgtccg
     2101 cacgaggatg aagttcgcgt cgctcgggta gacctgcacg acttccggca cgtcgcgtag
     2161 cgcggccgag agacgcgcgc gctcgcgctt cagcacgccg acgcgctcct cggagacctt
     2221 cagcgagtcc tgcgacagcg cctgctgcac gagctcgatc gacggcgtcg gaaacgtgta
     2281 cggcggcagc acgacctgcg cgaactcgat gagctcgggc gcggccacga tgaggccgca
     2341 gcgcacgccc gcgagcgaca cgaacttcga cagcgtccgc aggagcacga cgtgctcgta
     2401 gcgtgcgcgc agctcgagga agtcgccgcc ggtcgcgaac tcgtgataag cctcgtcgat
     2461 gacgacgagc gcgcggccgg cagcggcgcg gcaaatgcgc tcgatgtcgg cgcgcgccat
     2521 cgactggccg gtcggattgt tcggcgagca gatgaagacg attttcacgc tggcgtcgac
     2581 ggcggcgagg atcttgtccg tatcgagcgc gaaatcctgc tcggccttca gcggcacgcg
     2641 cacgacgccg gcgttctgga tgccggcgta gaggcggtac atgtcgaaag tcggcgggca
     2701 tacgaggatc ttgtcgcggc ccgcagcgca aaagccgcgg accagcacgt cgatggcctc
     2761 gctgctgccg cgcgtcacga ggatctgc
//
LOCUS       DASSNN010000467         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_82390, whole genome shotgun sequence.
ACCESSION   DASSNN010000467 DASSNN010000000
VERSION     DASSNN010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82390"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1404)
                     /locus_tag="VFL84_08730"
     CDS             complement(118..1404)
                     /locus_tag="VFL84_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HET7608745.1"
                     /translation="MLFALILAGLVAVLLTGVPVFAGLTLFGTAVLYAAEGHLGGLGD
                     LVFGELNTYLLVAIPLFTLMAQFMIRGKVVDDLFGAAHTLVRHLPGGLGVATVAACTV
                     FAAISGSSVATALTIGAAAIPLMVRYGYPARVAYGVVGAGGTLGILIPPSGPMILYGV
                     VSDTSIGALFMAGVVPGLMLATIFAVYCVSAARFGKLARIREPRASLPEIAAALRRSF
                     WALMLPVLVLGGMYFGVFTATEAAAIGALGALLIAMLVYRNFTLRDLWISAQDAARNT
                     AMLFMILAAAAILANVLTKLRIPNERVDLVLRYEVSQLGFLCAVMALIFVLGMFLETI
                     SIILITTPVVLPVLAALKIHPVWYGILLTINLELALITPPVGMNLFALKAITRAPILE
                     IIHGVAPYVLLLIGGLALVMLFPSIALWLPGTMNFR"
     gene            complement(1534..2058)
                     /locus_tag="VFL84_08735"
     CDS             complement(1534..2058)
                     /locus_tag="VFL84_08735"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HET7608746.1"
                     /translation="MARIERLVRALAHVGLALAALALIASLATISYSVIRRYVFGAPV
                     AWTDELAGYLLVASVMLAAGDALLGGEHISVDILTERLRPSGKRIAALIGLFTVAVTA
                     VLLLVEGIGTVEFSHQVGLRSNGYLALPMWMPQLLVPIGALLLLLAAIVSFVAVLRGG
                     PLPHERDMPAQGIE"
BASE COUNT          407 a          748 c          746 g          287 t
ORIGIN      
        1 gcgattgtgt cgcgcgcccg cgtatcgtgc aattttaccc gctttgcctc gacgacggcc
       61 acggcgggcc cggctgatca ggtggtgtcg cgggtgtgcg attgcaaccg cgacgcctca
      121 gcggaaattc atcgtccccg gcagccacag cgcgatcgac ggaaacagca tcaccagcgc
      181 aaggccaccg atcagcagca gcacgtacgg cgcaacgccg tggatgatct cgaggatcgg
      241 cgcgcgcgtg atcgccttga gcgcgaacag gttcatgccc accggcggcg tgatcagcgc
      301 gagctcgagg ttgatcgtga gcaggatgcc gtaccacacc gggtggatct tgagcgccgc
      361 gagcaccggc agcacgaccg gcgtcgtgat caggatgatc gagatcgtct cgaggaacat
      421 cccgagcacg aagatcagcg ccatcacggc gcagaggaag ccgagctgcg acacttcgta
      481 gcgcagcacg aggtcgaccc tctcgttggg gatccgcagc ttggtgagca cgttggcgag
      541 gatcgccgcc gcggcgagga tcatgaacag catcgcggtg ttgcgcgccg catcctgcgc
      601 cgagatccac aggtcgcgca gcgtgaagtt gcgatagacg agcatcgcga tcagcagcgc
      661 gcccagcgcg ccgatggcgg cggcctcggt tgccgtgaac acgccgaaat acatgccgcc
      721 gagcaccagc accggcagca tgagtgccca gaacgagcgg cgcagtgcgg cggcgatttc
      781 cggcagcgac gcgcgcggct cgcggatgcg cgccagcttg ccgaagcgcg cggcgctcac
      841 gcagtagacg gcgaagatcg tcgcgagcat cagcccggga acgacgccgg ccatgaacag
      901 cgcgccgatc gacgtgtcgg acacgacgcc gtacaggatc atcggcccgg aaggcgggat
      961 caggatgccg agcgttccgc cggcgccgac gacgccatac gcgacgcgcg ccggatagcc
     1021 gtagcgcacc atcagcggaa tcgccgcggc gccgatcgtc agcgcggtcg cgacgctcga
     1081 tcccgaaatc gccgcaaaca ccgtgcacgc ggcgaccgtc gcgacgccga ggccgccggg
     1141 cagatgccgc acgagcgtgt gggcggcgcc gaacaggtcg tcgacgacct tgccgcgaat
     1201 catgaactgc gccatcagcg taaacagcgg aatggcgacg agcagatacg tgttgagctc
     1261 gccgaacacg aggtcgccga gcccgcccag atgcccctcg gccgcataga gtacggcggt
     1321 gccgaacaac gtgagcccgg cgaacaccgg aacgccggtc aggagaaccg cgacgagccc
     1381 ggcgaggatc agcgcgaaca gcacgggcgt gtcaccccag cgctcactct cggcctccct
     1441 gcactttcgg cgcgaatggc atcgccggcg tgtcggcctc tctcgctcgc gcggcttcgg
     1501 gcccttcggg cctcgggctt cgcccagccg ctctcactcg atgccctgcg ctggcatgtc
     1561 gcgctcatgc ggcaacgggc cgccgcgcag cacggccacg aacgagacga tcgccgccag
     1621 cagcagcagc aacgcgccga tgggtaccag aagttgcggc atccacatcg gcagcgcgag
     1681 gtaaccattg gagcgcagcc cgacctggtg cgagaactcg acggtgccga tgccttcgac
     1741 gagcagcagc accgcggtca ccgccacggt gaacaggccg atgagcgccg cgatgcgctt
     1801 gccgctcggg cgcaggcgct cggtcaggat gtcgacggaa atgtgctcgc cgccgagcag
     1861 cgcgtcgccg gcggcaagca tgacgctcgc caccagcagg tagccggcga gctcgtcggt
     1921 ccatgcgacg ggcgcgccga aaacgtagcg gcgaatgact gaatacgaga tcgtcgcgag
     1981 cgacgcgatc agcgccagcg cggccagcgc gaggccgacg tgcgcgagcg cgcgcaccag
     2041 ccgttcgatg cgcgccatgg cggcttgtta cgcccgcgag ccttcggtca gagcgacttc
     2101 acgagatcga ggatcttctg cccgtcctgt ggcgccgact tcaggaacgc gtcgagcacg
     2161 ggcttctgca tcgccgcctt ccacgtcg
//
LOCUS       DASSNN010000468         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_823098, whole genome shotgun sequence.
ACCESSION   DASSNN010000468 DASSNN010000000
VERSION     DASSNN010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823098"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1287)
                     /locus_tag="VFL84_08740"
     CDS             complement(175..1287)
                     /locus_tag="VFL84_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HET7608747.1"
                     /translation="MTDKPGLDYAAAGVDYGTLDASKILAQKAARATAVNLAAHDAVE
                     IEASRGESAYLVQVGGVLIASITECLGTKALVADAVRAYSGRTHYDTLAQDTIAMAVN
                     DLVTVGATPLSTHAYWSAGSSDWFADEERMRDLVRGWQAACDVCGMAWGGGETPALGG
                     VVEAQRIDLAASCVGIVKAQDRLMLGERLKAGDAIVLLASSGIHANGISLARKLAERL
                     PEGYRTPLDDGRSFGEALLDPTVLYPPVIEALFAAGVRPHYAVNITGHGWRKLMRHPA
                     DLKYVIRRIPPVPPVLEFLHKQGGMSARDAYGTLNMGAGFALFVAREDVAATIGVAQA
                     AGLPAWHAGDVEAGEKRLVIEPLGLQYAGSDLHLRA"
     gene            complement(1338..>2053)
                     /gene="aroE"
                     /locus_tag="VFL84_08745"
     CDS             complement(1338..>2053)
                     /gene="aroE"
                     /locus_tag="VFL84_08745"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET7608748.1"
                     /translation="YELIDAEPTAFETAVRGFAAAGGKGLNVTVPHKQAAFALCTKLS
                     DGATAAGAVNTISIVDGGLHGHNTDGIGLVRDLKINLRRALAGKRILVLGAGGAARGV
                     IGPLLDEKPEELVVANRSKDRADEVAANFAGTVLTARSFDELAYLRPFDVVINATSAG
                     LKGEAPPFPPALVGPRSFCYDLVYGSADTPFVAWAKTHGAEGAVHGWGMLVEQAAESF
                     AIWRGVRPDTKGLLKQVPR"
BASE COUNT          290 a          731 c          701 g          331 t
ORIGIN      
        1 cacggcgccg tcgttctgct gccgctttcg ccgctgccat tggcagtagc cgtagccgcg
       61 gttttcggct gcgtgtacct tctgctcgcg ctcgcgcttc ggcttccgga agcgcgcggc
      121 ctcacggata cgattcggcg ccgcttgcgg cgctgagccg cacgcgcgtt cccctcacgc
      181 tcgcaggtgc agatcgctgc cggcgtactg caacccgagc ggctcgatca cgaggcgttt
      241 ctcgccggcc tcgacgtcgc cggcatgcca tgcaggcagc ccggcggctt gggcgacgcc
      301 gatcgtcgcc gcgacatcct cacgcgccac gaacagcgcg aagcccgcgc ccatgttcag
      361 cgtgccgtag gcatcgcgcg cgctcatgcc gccctgcttg tgcaggaact cgagcaccgg
      421 cggcaccggc ggaatgcgcc ggatcacgta cttgagatcg gccgggtgac gcatgagctt
      481 gcgccagccg tggccagtga tgttgacggc atagtgcggg cgcacgccgg ccgcgaacaa
      541 tgcctcgatg acgggcgggt acagcaccgt cgggtcgagc agcgcctcgc cgaagctgcg
      601 gccgtcgtcg agcggcgtcc gatagccttc cggcaaacgc tcggcgagct tgcgcgcgag
      661 gctgatgccg ttcgcgtgaa tcccgctcga cgcgagcagc acgatcgcat cgccggcctt
      721 caagcgctcg ccgagcatca agcgatcttg ggctttgacg atgccgacgc acgacgccgc
      781 caagtctatg cgctgcgcct cgacgacgcc gccgagcgcg ggcgtctcgc cgccgcccca
      841 cgccatgccg cagacgtcgc acgccgcttg ccaaccgcgc acgaggtcgc gcatgcgctc
      901 ctcgtcggcg aaccagtcgc tgctgcccgc ggaccagtac gcgtgcgtcg acagcggcgt
      961 cgcgccgacc gtgacgagat cgttgacggc catcgcaatc gtgtcttgcg cgagcgtgtc
     1021 gtagtgggtt ctgcccgagt acgcgcgtac cgcatccgcg acgagcgctt tcgtgccgag
     1081 gcactccgtg atcgacgcga tcaggactcc gccgacctgg acgaggtatg ccgattcccc
     1141 gcgcgacgct tcgatctcga ccgcgtcgtg cgccgcgagg ttgaccgccg tggcgcgcgc
     1201 cgctttttgc gcgaggatct tcgaggcgtc gagcgtgccg tagtcgacgc cggcggcggc
     1261 gtagtcgaga ccgggcttgt cagtcatagg gctccgcggt ttaggcccat cataccgggc
     1321 gcagcgcggg ggatcgccta ccgcgggact tgcttcagca gccccttcgt gtcggggcgc
     1381 acgccgcgcc aaatcgcgaa cgactcggcc gcctgctcga cgagcattcc ccagccgtgc
     1441 acggcgcctt ccgcgccatg cgtttttgcc caggcgacga acggcgtgtc cgccgagcca
     1501 tagacgaggt cgtagcaaaa gctgcgcggg ccgacgagcg ccggcgggaa cggcggcgcc
     1561 tcgcccttga ggcccgccga cgtcgcgttg atgacgacgt cgaacggccg caggtaggcg
     1621 agctcgtcga acgagcgcgc ggtcagaacc gtgccggcaa aattcgcggc gacctcgtcg
     1681 gcgcggtctt tgctgcggtt cgcgacgacg agctcctccg gcttctcgtc gagcagcgga
     1741 ccgatgacgc cgcgcgccgc gccgccggcg ccgagcacga ggattcgctt cccggcgagc
     1801 gccctgcgca gattgatctt cagatcgcgg acgaggccga tgccgtcggt gttgtggccg
     1861 tgcaggccac cgtcgacgat gctgatggtg ttgacggcgc ccgcggcggt cgcgccgtcg
     1921 ctcaacttcg tgcacaacgc gaacgcggct tgcttgtgcg gcaccgtgac gttcaagccc
     1981 ttgccgcccg cagcagcgaa gccgcggacg gccgtctcga atgccgtggg ctccgcgtcg
     2041 atgagctcgt acg
//
LOCUS       DASSNN010000469         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1094247, whole genome shotgun sequence.
ACCESSION   DASSNN010000469 DASSNN010000000
VERSION     DASSNN010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094247"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VFL84_08750"
     CDS             <1..790
                     /locus_tag="VFL84_08750"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET7608749.1"
                     /translation="APAGAAAGLGCALGRHVRMLDPKAMNVRLAAVPAIVAALLTAAC
                     SGALQTGAPEPTATALEPQPAPARRAAPDPTAAERRIASLELQLLEKGAQIQNLQEQL
                     DEARREVVRSMARVQSVASRAEAASGIAEAELALQSMPDNAEIRKLTELASAEFEDEH
                     WGGALWLANQAKSAALNARGQLAEVDDGTLRPYERTLSLPLELETTTGANVRSGPGTG
                     FGVVYTLAPRSRVVAYSSAEQWLRIVDDSGRRGWISQSLIRGRP"
     gene            complement(771..>2332)
                     /locus_tag="VFL84_08755"
     CDS             complement(771..>2332)
                     /locus_tag="VFL84_08755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7608750.1"
                     /translation="LATLLPSVGTAWISYLENKRALEAKASEEIMAVSAQTAREVDLW
                     AKERRYDLRVFASSYEITENIESARGGSTSANRRVTDYLKSVSERFDVYDELLILAAP
                     SGQVIATTGDRAASVKLPEGWQTRLGKGDFVAGTPYWDDVDQRAEIVIAVPIQAGERI
                     LGTLAAEVNLLALIETLKGFAPGESGLASLLTADGSLIVSSAGGSAQAMQMRYPRETI
                     QAQLQSDVAPVELTDVTGEPVIGSMRPVPGIDWIVVAAIPTAEVYAQLARMRLVTVAI
                     VAGTLLVAALVGYALGLIITRPLDRLTRAASKVAGGELEVELVDTKGGEVGYLTEVFR
                     DMVSRLKTSRDELERLSVTDPLTGLDNRRRMTEALQNEVLRSRRLEHPFSVVMVDVDH
                     FKAYNDAHGHMAGDEALKRVATVLRGALSDGDSVARYGGEEFFVLLPETAATAAADFA
                     KRVRAALEKHAPPAGPVTLSFGIAAYPAHGDSGEALIRAADAALYEAKRGGRDRVVVA
                     RGEERVKAARG"
BASE COUNT          360 a          825 c          789 g          358 t
ORIGIN      
        1 cgcgccggcc ggcgccgcgg cggggttagg ctgcgcgctc gggaggcacg tgcggatgct
       61 cgaccccaag gccatgaacg ttcgtcttgc ggcggtgccg gcgatagtcg cggcgctgct
      121 gacggccgct tgcagcggcg cccttcaaac cggcgcgccg gagcccaccg ctacggcgct
      181 cgagccgcag ccggcacccg cgcgtcgcgc cgcgccggac ccgaccgccg cggagcggcg
      241 tatcgcgagc ctcgagctgc aactgctcga aaaaggcgcg cagatccaaa atcttcagga
      301 gcagctcgac gaggcgcgcc gcgaggtcgt gcgctcgatg gcgagagtgc aatccgtcgc
      361 ctcgcgcgcc gaagccgcat cgggcattgc cgaggccgag ctcgcgctgc agtcgatgcc
      421 ggataacgcc gaaattcgca agctgacgga gctcgcgagc gcggaattcg aggacgagca
      481 ctggggcggt gccctgtggc tcgctaacca ggccaagagc gccgcgctca atgcgcgcgg
      541 gcagctcgcc gaagtggacg acggcacgct tcgaccgtac gaacggacgc tgtcgctacc
      601 gctcgagctc gagacgacga cgggcgccaa cgtgcgctcg gggccgggaa cgggtttcgg
      661 cgtggtctat acgctcgcgc cgcgaagccg cgtcgtcgcc tactcctccg cggagcagtg
      721 gctgcgcatc gtcgacgatt ccggccgccg cggctggatc agccagagcc tcatccgcgg
      781 gcggccttga cgcgctcttc cccgcgcgcg acgacgacgc ggtctcttcc gccacgcttc
      841 gcctcgtaga gcgccgcgtc cgcggcgcgg atcagcgctt cgccgctgtc gccgtgggca
      901 gggtaagccg cgattccgaa gctcaacgta acgggccccg ccggcggcgc gtgcttttcg
      961 agggcggctc gcacgcgctt agcgaaatcc gcggccgcgg tcgcggccgt ctccggcaac
     1021 agcacgaaga actcctcgcc gccgtagcga gcgaccgagt cgccgtcact gagcgctccg
     1081 cgcagcaccg tcgcgacgcg cttcagcgct tcgtcgccgg ccatgtggcc atgcgcgtcg
     1141 ttgtacgcct tgaagtggtc gacgtcgacc atgacgacgg agaacgggtg ctcgagacgg
     1201 cgcgaacgca gcacctcgtt ctgcagcgct tcggtcatgc ggcgccggtt gtccaggccg
     1261 gttagaggat ccgtgacgga cagccgctcg agctcatcgc ggctcgtctt gagccgcgag
     1321 accatgtctc tgaagacctc ggtcagatag ccgacttcgc cgcccttcgt gtcgacgagc
     1381 tcgacctcga gctcgccgcc cgcgaccttc gacgccgcgc gtgtcagcct gtcgagcggg
     1441 cgagtgatga tgaggccgag cgcgtagccg accagggccg cgaccagcag cgttcccgcc
     1501 acgatcgcca ccgtcacgag ccgcatgcgg gcaagctgcg catagacctc ggctgtcgga
     1561 atcgcggcca cgacgatcca gtcgatgccg ggcaccggcc gcatgctgcc gatgaccggc
     1621 tcgccggtca cgtcggtgag ctcgaccggc gctacatcgc tctgcagctg cgcctgtatc
     1681 gtctcgcgcg gatagcgcat ttgcatcgcc tgcgccgagc cgcccgccga gctcacgatc
     1741 aagctgccgt ccgcggtcaa cagcgacgcg aggccggact ctccgggcgc aaagcccttc
     1801 agcgtttcga tgagggcaag cagattgacc tcggccgcga gcgtgccgag gatgcgttcg
     1861 ccggcctgaa tcggaacggc gatgacgatc tcggcgcgtt gatcgacgtc gtcccagtac
     1921 ggcgtgcccg cgacgaaatc gcctttacca aggcgcgtct gccaaccctc gggcagcttg
     1981 accgaagcgg cgcggtctcc cgtcgtcgcg atgacctggc cgctcggcgc cgccaagatc
     2041 agcagctcgt cgtagacgtc gaaccgctcg ctgacggact tcaaatagtc ggtcacgcgc
     2101 cgattggcgc tcgtcgaccc gccgcgcgcg ctctcgatgt tctcggtgat ttcgtacgag
     2161 ctcgcgaaca cgcgcaggtc gtagcgtcgt tccttggccc agaggtcgac ctcgcgcgcg
     2221 gtctgcgcgc tgacggccat gatctcttcg gaagccttcg cttcgagggc gcgcttgttc
     2281 tcgagatacg agatccacgc ggtaccgacc gacggcaaca gcgtggcgag ca
//
LOCUS       DASSNN010000470         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_586143, whole genome shotgun sequence.
ACCESSION   DASSNN010000470 DASSNN010000000
VERSION     DASSNN010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586143"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFL84_08760"
     CDS             complement(<1..299)
                     /locus_tag="VFL84_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HET7608751.1"
                     /translation="MLFLTALAAHAQAHEAACSALAKWTGGADKGVQISEARFYTDRT
                     IQGPGGSQTLPPHCHVAGSFERRTGVEGKEYAIGFALNMPANWNGRFMFQGGGGL"
     gene            complement(378..1244)
                     /locus_tag="VFL84_08765"
     CDS             complement(378..1244)
                     /locus_tag="VFL84_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608752.1"
                     /translation="MIAAAAVAATLSPAAVAQSASNGRNATDAGSANNAAPFTPGRLW
                     DGKTPDFRGIWQVQDTAYVNIEGHPSEDGLPASASIIVDPPDGKIPYKPAALARRQEN
                     YRNRATADPSSKCYQAGVPRATYLPTPLQILQSPGNFAIVYQDNHAFRVFHPDTRPHF
                     DAADFWMGDTRYRWEGDTLVADVVALTDELWLDHAGNFHSTGVHIVERYRMTGADTLE
                     YEARIEDPEVYTRPWTLRTVLHRVKQPGARIIEDECLEDENGVRHHISPYNPANLLKH
                     DYSRWKKPAGSD"
     gene            complement(1304..2176)
                     /locus_tag="VFL84_08770"
     CDS             complement(1304..2176)
                     /locus_tag="VFL84_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608753.1"
                     /translation="MLAKFDEARRPATVAAIYLSMLLAAASPRLVLAQPAPQSAPANA
                     VLPRLPDGHPDLQGLWLKTAGGFQGLLIGSLDGTNFAGGGGRGGARRAAAGPPPGPRF
                     EYTPEAAAEKLDREHRGYDDPEAHCHLPGVPRALDQPGGLYPVQIIQDENYIALLHEA
                     MHDVRIIPTDDSPHPKNYWAWDGDSRGRWEGDVFVVDVSDFNGRTWLDMAGDFVDENE
                     HVLERFTLLDHDTILYEATVTDPTVFVKPAEFRFTLKRLPADQQILEYSCLEGERSLQ
                     HYTKEDGGKKRRVE"
     gene            complement(2178..>2591)
                     /locus_tag="VFL84_08775"
     CDS             complement(2178..>2591)
                     /locus_tag="VFL84_08775"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7608754.1"
                     /translation="QVLPIAMLAFSWGAAPAFAHHSFVAEFDARKPVTLKGVVVKWEM
                     INPHGWITLDVPAADGATARWMIETSNPNAFMRLGWTKNSLKPGDPITIDAYRAKDGS
                     NTANAARITLANGAKVFAGSVGTPRVEAGDQSSVH"
BASE COUNT          420 a          834 c          853 g          484 t
ORIGIN      
        1 ttcaatccgc cgccgccctg gaacatgaac cgtccgttcc agttggccgg catgttgagc
       61 gcgaagccga tcgcgtactc cttgccctcg acgcccgttc gccgctcaaa gctgccggcc
      121 acgtggcagt gcggcggcaa ggtttggctg ccgcccggtc cctgaatagt gcggtcggta
      181 tagaaccggg cttcggagat ttgcacgcct ttgtcggcgc cgccggtcca tttggccaac
      241 gcgctgcagg ccgcctcgtg cgcctgtgcg tgcgccgcga gcgccgtgag gaagagcatc
      301 gtcacaggaa cgagtcgagc accattcatc gtgcggtctc caagcttagg ggtctaacga
      361 cgcctatttg cgaagcacta atccgaaccc gcaggcttct tccatcgcga gtaatcgtgc
      421 ttcaacaggt tcgccggatt gtatggcgaa atgtgatgac gaacgccgtt ctcgtcctcc
      481 aaacactcgt cctcgatgat tcgggcgccg ggctgcttga cccgatgcaa gaccgttcgg
      541 agcgtccacg gccgcgtata gacctcgggg tcttcgatgc gagcttcgta ctcgagcgtg
      601 tctgcgcccg tcatccggta gcgctcgacg atgtggacac cggtgctgtg gaagttgccg
      661 gcgtgatcga gccaaagctc atcggtgagc gccacgacgt cggcgacgag ggtgtcgccc
      721 tcccaacggt aacgcgtatc gcccatccaa aagtcagcgg cgtcgaaatg ggggcgcgtg
      781 tcgggatgga agacgcggaa ggcgtgattg tcctggtaga cgatcgcgaa gttgccggga
      841 ctttgcagga tctggagcgg cgtagggaga tacgtcgcac gggggacgcc ggcctgatag
      901 cacttcgagg acgggtcggc ggttgcgcga ttgcggtagt tctcttggcg ccgcgccaac
      961 gccgcaggct tgtacggaat cttcccgtcg ggcggatcga cgatgatgct cgccgacgcc
     1021 ggcaggccgt cctcgctcgg atgaccctcg atgttcacgt aggccgtgtc ttgcacttgc
     1081 cagatcccgc ggaagtccgg cgtctttccg tcccagagcc ggcccggcgt gaacggcgcc
     1141 gcgttgttcg cgctcccggc gtcggtcgcg ttcctgccgt tgctcgcgct ctgcgcgacg
     1201 gccgcgggag aaagcgttgc ggcgacggcg gccgccgcga tcacggcaat cgttcctgca
     1261 gcggcaaccg ccgaggcgcg gagcgcttct cggcgagcag cgatcattcg accctccgct
     1321 tcttgccgcc gtcttccttg gtgtagtgct gcaagctgcg ctcgccttcg aggcagctgt
     1381 attcgaggat ctgctgatcc gctggaaggc gtttcagggt gaagcgaaac tcggcgggct
     1441 tgacgaatac cgtcgggtcc gtgacggtgg cctcgtagag gatcgtgtca tgatccaaga
     1501 gcgtgaatcg ctcgagcaca tgctcgttct cgtcgacgaa gtcgcccgcc atgtcgagcc
     1561 aggtgcggcc gttgaagtcg ctcacgtcga cgacgaacac gtcgccttcc cagcggccgc
     1621 gcgaatctcc gtcccaggcc cagtagttct tcggatgcgg gctgtcatcc gtgggaatga
     1681 tgcgcacgtc gtgcatcgct tcgtgcagca gcgctatgta gttctcgtcc tgaatgatct
     1741 gcacgggata cagcccgccc ggctgatcga gtgcacgcgg gacgcctggc aaatggcaat
     1801 gcgcctcggg gtcgtcgtaa ccccggtgct cgcgatcgag tttttccgcc gcggcctccg
     1861 gcgtgtattc gaatcgcggc ccgggcggcg ggcccgccgc cgcgcgccgg gcgccgcctc
     1921 tgccgccgcc cccggcgaaa ttcgtcccgt cgagcgaccc gatgagcaag ccttgaaacc
     1981 cgccggcggt cttgagccac agaccctgta agtcgggatg gccgtcggga agcctcggga
     2041 gcaccgcgtt ggccggagcc gactgcggcg cgggctgagc caaaaccaat cgaggcgagg
     2101 cggccgcgag cagcatggac aagtagattg ccgcgacagt cgccggtcgg cgcgcctcgt
     2161 cgaactttgc gagcacgtta gtgcaccgaa gactgatcgc ccgcctcgac cctaggagtg
     2221 cccacggagc ccgcgaacac tttggcgccg ttcgcgagcg tgatgcgcgc ggcgttcgcc
     2281 gtgttcgaac catccttggc ccgataggcg tcgatcgtta tcgggtcccc gggcttaagc
     2341 gaattcttcg tccatccaag ccgcatgaaa gcgttcgggt tcgaggtctc gatcatccat
     2401 cgcgccgtcg cgccgtccgc cgcggggacg tcgagcgtga tccagccgtg cggattgatc
     2461 atctcccact tcacgacgac gcctttgagt gtcaccggct tgcgcgcgtc gaactcggca
     2521 acgaacgagt ggtgggcaaa cgcgggcgca gcgccccagg agaacgcgag catcgcaatc
     2581 ggcaatacct g
//
LOCUS       DASSNN010000471         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1595653, whole genome shotgun sequence.
ACCESSION   DASSNN010000471 DASSNN010000000
VERSION     DASSNN010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595653"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFL84_08780"
     CDS             <1..441
                     /locus_tag="VFL84_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7608755.1"
                     /translation="RNNVIPSEAKVTLDERMRPGEDPEAFRAELVRLIDDDDIDVVWN
                     GFGERPPGLISPVDDPAFVAIERAVREHYGAPVVPMMSTGATDMAFLRAKNMRCFGIG
                     PATDQEDGPLGYGAHSDQERILESELYRFVHFSYDLVVDLAGAD"
     gene            complement(448..1869)
                     /locus_tag="VFL84_08785"
     CDS             complement(448..1869)
                     /locus_tag="VFL84_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET7608756.1"
                     /translation="MGVQADSLEYERVASIIERQIDTGAFRAAERVPSLRSMSRHAGV
                     SIGTVVQAYLHLEQRGLLEARPRSGYFVAKRGTQPLALPTPRKAETRPRGVSREVVDT
                     VIESFGRTDLVALGSAVATSASRINGRLNGLTRRTLRDMPTLPNVFSSPPGLAELRTE
                     VAKRMALCGLPSRADDVVITNGTMEALALSLRVLCEPGDAVLVESPTYFGILQLAEHM
                     RLKVVEVPNHAGLGLDADALERALRSTRVAAAILQSSFNNPTGALTPDAAKRRIVAAL
                     AKHGVPLIEDDIYSDLHFGRERPTPFAAFDDSGNVITCGSMSKSIAIGYRIGWAISTR
                     YTEALIRAKFCTTVGCPTLQQHVAARYFRAGLHERHLRHVRNHLRGNCERFAEAIAAS
                     FPSGTRASQPAGGVVLWVELPRGTDGVALFHAALERGIGIAPGLIFSAAGEYRNFIRM
                     SAGMQWTPAVAKAVALLGRLAGR"
     gene            1958..2548
                     /locus_tag="VFL84_08790"
     CDS             1958..2548
                     /locus_tag="VFL84_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HET7608757.1"
                     /translation="MELLLPLASFAFVSSITPGPNNLMLSASGVVFGFNRTLPHLLGV
                     AVGFTLLLFVCASGVGALLLGLPAAALVLKVFGTAYLLYLAWSYRNALAPEARSASAR
                     PMKFAEAVAFQFVNAKAWIMGLTAASVFVPDYEPRYLAIAVMCGVFAIVNLPCIATWA
                     ALGATLKRCLTQDRWRKIFSGAIALLMVYSVIAMWV"
     gene            2685..>3060
                     /locus_tag="VFL84_08795"
     CDS             2685..>3060
                     /locus_tag="VFL84_08795"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET7608758.1"
                     /translation="MDIKAPDIHAFFAAAGAFAAAADKVDDEMALRLGSRFNVFDVIR
                     PNENRISDILRDLLDPHGMHGQGDRFLRAFLTLCTIEAAWCSPRASVRVEREHRTTML
                     DSARRIDLVLHFAGTEARGAIAI"
BASE COUNT          474 a         1020 c         1045 g          521 t
ORIGIN      
        1 cgcaacaacg tgattccgtc cgaggccaaa gtgacgctcg acgagcgcat gcgccccggc
       61 gaagaccccg aggcgttccg cgccgagctc gtgcgcctca tcgatgacga cgacatcgac
      121 gtcgtctgga acggcttcgg cgagcggccc ccgggcttga tctcgccggt cgacgacccg
      181 gcgttcgtcg cgatcgagcg cgcggtccgc gagcactacg gcgcgccggt cgtgccgatg
      241 atgagcacgg gcgccaccga catggcgttt ctgcgcgcca agaacatgcg ctgcttcggc
      301 atcggccccg cgaccgacca agaggacggc ccgctcggct acggcgcgca cagcgaccag
      361 gagcgcatct tggagtcgga gctctatcgc ttcgtgcact tcagctacga tctcgtcgtc
      421 gacctcgcgg gcgccgactg agcggcgcta acggccggcc aaacggccga gcagcgcgac
      481 ggccttcgcg accgcgggcg tccactgcat gccggcgctc atgcggatga agtttcggta
      541 ctcgcccgcc gccgagaaga tgaggccggg cgcgatgccg ataccgcgct cgagcgccgc
      601 gtgaaacagc gcgactccgt ccgtgccgcg gggcagctcg acccacagca cgacgccgcc
      661 ggccggctgc gaggcgcgcg tgccgctcgg gaacgaggcg gcaatcgctt cggcgaaccg
      721 ctcgcagttg ccgcgcaggt ggttgcgcac gtggcgcaga tgccgctcgt gcaggcccgc
      781 gcgaaagtag cgcgccgcga cgtgctgctg cagcgtcggg cagccgaccg tcgtgcaaaa
      841 cttcgcgcgg atcaacgcct cggtgtagcg cgtgctgatc gcccaaccga tccggtagcc
      901 gattgcgatc gacttcgaca tcgagccgca ggtgatgacg ttgcccgagt cgtcgaaggc
      961 cgcgaacggc gtcggccgct cgcggccgaa gtgcagatcg ctgtagatgt cgtcctcgat
     1021 cagcggcacg ccatgcttcg cgagcgcggc gacgatgcgg cgtttcgcgg cgtccggcgt
     1081 cagcgcgccc gtcgggttgt tgaagctcga ttgcaagatc gcggccgcga cgcgcgtgct
     1141 ccgcagggcg cgctcgaggg cgtcggcgtc tagacctaat cccgcgtgat tcggcacttc
     1201 gacgaccttg agccgcatgt gctcggcaag ctgcaggatg ccgaaatagg tcggagactc
     1261 gacgagcacg gcgtcgccgg gctcgcacaa gacgcgcagc gacagcgcga gcgcctccat
     1321 cgtcccgttc gtgatgacga cgtcgtcggc gcgcgagggc aggccgcaca gcgccatgcg
     1381 cttcgcgacc tccgtgcgca gctcggcgag ccccggcggc gacgagaaca cgttcggcaa
     1441 cgtgggcatg tcgcgcagcg tccggcgcgt caagccgttc aaccggccgt tgatgcggga
     1501 cgccgaagtg gcgaccgcgc tgccgagcgc gaccaagtcg gtgcgcccga acgattcgat
     1561 gacggtgtcg acgacctcgc gtgagacgcc gcgcggccgc gtctcggcct tgcgcggcgt
     1621 cggcagcgcg agcggctgcg tgccgcgctt cgcgacgaag tagccggaac ggggccgcgc
     1681 ctcgagcagt ccgcgctgct cgagatgcaa atacgcctgc acgaccgtgc cgatgctgac
     1741 gccggcgtgg cggctcatcg agcgcagcga cggcacgcgc tcggcggcgc gaaacgcgcc
     1801 ggtatcgatc tgccgctcga tgatcgacgc gacgcgctcg tattcgaggc tgtctgcttg
     1861 aacgcccatc tgtgctagtc gaaaaacacc aaaagtgagt ctgtactata agctgaggcg
     1921 cacgtaactt gcgacctccc cattgggagg tacggccatg gagctgttgc tgccgctcgc
     1981 gagcttcgcg ttcgtgtcgt cgatcacgcc gggcccgaac aacctgatgc tctcggcctc
     2041 gggcgtagtg ttcggcttca atcgcacgct tccgcacctg ctcggcgtgg ccgtcggctt
     2101 cacgctgctg ctcttcgtct gcgccagcgg cgtcggcgcg ttgctgctcg ggctgccggc
     2161 cgccgcgctc gtcttgaagg tgttcggcac ggcgtacctg ctctatctcg cgtggtcgta
     2221 ccgcaatgcg ctcgcgcccg aggcgcgcag cgcgtccgcg cggccgatga agttcgcgga
     2281 agccgtcgcg ttccagttcg tgaatgcgaa ggcctggatc atggggctta ccgcggcgtc
     2341 tgtcttcgtg ccggactacg agccgcgcta tctggcgatc gcggtcatgt gcggggtttt
     2401 cgcgatcgtg aacctaccgt gcatcgcgac gtgggcggcg ctcggcgcga cgttgaagcg
     2461 ctgcttgacc caggaccgct ggcgcaagat cttcagcggc gcaattgcgc tgctcatggt
     2521 ctactcggtg atcgcgatgt gggtctgaca tcgccgggtg ctgtccgtat atcgcgtcag
     2581 tgcgacgtag ctctcttcgc cgctgcgcga ccaatactac acttcggcgt caggcgcttc
     2641 ggattggtgg ccagatgatg ctcaagaacc tggcgggcta gcggatggac attaaagcgc
     2701 cggacatcca tgccttcttc gcagcggccg gcgcctttgc cgcggcggcg gacaaggtag
     2761 acgacgagat ggcattgcgg ctcgggagcc gcttcaacgt gttcgatgtt atccggccaa
     2821 acgagaatag gatttccgac atcctccgtg atcttctcga tcctcacggc atgcatggac
     2881 agggtgaccg tttcctacgg gcgttcctga cgctctgcac gatcgaggcc gcatggtgct
     2941 cgcctcgcgc gtccgtgaga gtagagcgcg agcatcggac gacgatgctc gattcggctc
     3001 ggcgcatcga tctcgtgctg cactttgcag gcaccgaggc gcgcggcgcc atcgcgattg
//
LOCUS       DASSNN010000472         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_586534, whole genome shotgun sequence.
ACCESSION   DASSNN010000472 DASSNN010000000
VERSION     DASSNN010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586534"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1219)
                     /locus_tag="VFL84_08800"
     CDS             complement(164..1219)
                     /locus_tag="VFL84_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7608759.1"
                     /translation="MRFKPLALAFCCLAAVSLAVSSFAQPRTPPREIMQVKGDLYRAR
                     NGNWYTVFLVTPAGIILGDPINETFSPWLKGELDTRFPGKPVRYVVYSHSHFDHAAGG
                     AVFADTATFVAHENMLRNMDGRYPQMPGDMIDRNLNGVIDREEIDIPTNTRPGVCGMG
                     PGFFDGIDRNRDGVATPQELQQFIVKPTIVYNDRMRIELGGQVVELVHPGENHSNDAT
                     VMVFPAERAAFATEFIADALVAGNPRSLPSACGPFDGSPLEEWIKSYRTVESLDFDVM
                     VTGHGSQLVDKALVRETREFFEDLRSEVAAGMAAGRTLEQLKESVRLEKYKDWANYER
                     LRANNVEAAYRNLLTYR"
     gene            complement(1310..2068)
                     /locus_tag="VFL84_08805"
     CDS             complement(1310..2068)
                     /locus_tag="VFL84_08805"
                     /inference="COORDINATES: protein motif:HMM:NF038116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor H family protein"
                     /protein_id="HET7608760.1"
                     /translation="MNARLLASLAAASFVFGSSVYAGETAAGTEERITRNAAGRVDEH
                     AADELRDPTSFELLRRKPGEIAVKPRAAAGSLKGAQKPLTSGESWIYDARTDLFGDAD
                     NDGYYRFVRVQLDADTIHTFAEIYAEIYLSADGEAWELLYSTNNFSIFGTDDGDDYEV
                     ETELVSGYSTGRYDVLVEIYDADTDEFLDDFGPEDSELFSMVPLEDSGRDGIVVDVPP
                     PVTIVEESGGGAVSWLALAGLAGALSMRRRRRNA"
BASE COUNT          405 a          754 c          772 g          396 t
ORIGIN      
        1 ttcgtcctcg agcagccgga agccgacgga gtgctcgccg gcgggaaggc caaagacttc
       61 aggagcaggt tgcggaagcg acggcagcgc tgaaactgcg gcgagcagca gcgttgcgaa
      121 gcgcacgagt ggcatgggca actcctcggc cgcgcggacc ctctcagcga tacgtcagca
      181 gattccgata cgcggcctcg acattgttcg cgcgcagccg ctcgtagttc gcccaatcct
      241 tgtacttctc gagccgcacg ctctccttga gctgctcgag cgtccggccg gcggccatgc
      301 ccgccgcgac ctcgctgcgc agatcttcga agaactcgcg cgtctcgcgc acgagcgctt
      361 tgtcgacgag ctgcgagccg tgccccgtga ccatgacgtc gaagtcgagg ctctcgacgg
      421 ttcgatacga cttgatccat tcctcgagcg ggctgccgtc gaacggcccg cacgcgctcg
      481 gcagcgagcg cggattgccg gcgacgagcg cgtcggcgat gaactcggtc gcgaacgcag
      541 cgcgctcggc cggaaatacc atgaccgtcg cgtcgttaga gtggttctcg ccggggtgaa
      601 cgagctcgac gacttggccg ccgagctcga tgcgcatgcg gtcgttgtag acgatcgtcg
      661 gcttgacgat gaactgctgc agctcctgcg gcgtcgcgac gccgtcgcgg ttgcgatcga
      721 tgccgtcgaa gaagcccggg cccatgccgc agacgccggg ccgcgtgttc gtcgggatgt
      781 cgatctcctc gcggtcgatg acgccgttca agttacggtc gatcatgtcg cccggcatct
      841 gcgggtaacg gccgtccatg ttgcgcagca tgttctcgtg cgcgacgaac gtcgcggtgt
      901 cggcgaacac ggcgccgccc gcggcgtgat cgaaatggct gtggctatag acgacgtagc
      961 gaaccggctt gccggggaag cgcgtatcga gctcgccctt caaccacggc gagaacgtct
     1021 cgttgatcgg gtcgcccaag atgatgccgg ccggcgtcac gaggaacacc gtgtaccagt
     1081 tgccgttgcg cgcgcgatag agatcgcctt tgacttgcat gatctcgcgg ggcggcgtgc
     1141 gcggttgcgc aaacgacgac acggcgagcg aaacggcggc gaggcagcag aaggcgagag
     1201 ctagcggctt gaagcgcacc gggcacctga tgcgaagcga agtgccggga gtatagagca
     1261 gcggcggcga gcaatgcccg ccgccgctca agtgcaaccg cggccgcggt tacgcgttgc
     1321 ggcgtcggcg gcgcatcgag agcgcgccgg ccaagcccgc cagcgcgagc cacgagaccg
     1381 cgccgccgcc gctttcctct acgatcgtga cgggcggcgg aacgtcgacg acgatgccgt
     1441 cgcggcccga gtcctcgagc ggcaccatcg agaacagctc cgaatcctcc ggcccgaagt
     1501 cgtccaggaa ctcgtcggtg tcggcgtcgt agatctcgac gagcacgtcg tagcgaccgg
     1561 tcgagtatcc cgagacgagc tcggtctcga cctcgtaatc gtcgccgtca tcggtgccaa
     1621 agatcgagaa gttgttcgtc gagtagagca gctcccacgc ctcgccgtcg gcgctcaaat
     1681 agatctcggc gtagatctcg gcgaacgtat gaatcgtgtc ggcgtcgagc tgcacgcgca
     1741 cgaagcggta atagccgtcg ttgtcggcgt cgccgaagag gtcggtccgc gcgtcgtaga
     1801 tccacgactc gccgctcgtc agcggctttt gcgcgccctt gaggctgccc gccgcggcgc
     1861 gcggcttcac cgcgatctcg ccgggcttgc gccgcagcag ctcgaacgac gtcggatcgc
     1921 ggagctcgtc ggccgcatgc tcgtcgactc ggcccgcggc gttgcgcgtg atgcgctctt
     1981 ccgtgccggc cgctgtttcc ccggcataca cactcgaccc gaagacgaag ctcgcagcgg
     2041 cgagcgaagc cagcaagcgc gcgttcatgt tcagtcctcc cggcacgcca agtgccgtcg
     2101 aggaccattt agcgacgcga atgctgaacg gcacctgagg ccgctcgaac gccaagacta
     2161 tggaaaatcg cgcctctaaa aggggacaga tctattttcc aacgcctcta aaaggggaca
     2221 gatctatttt ccaacgaaaa tagatctgtc cccttttagg gcgtcccttt ttagggcggg
     2281 tctagcgctc ggtcttggct tcgccgacgg gcacggagtc gatggcg
//
LOCUS       DASSNN010000473         3164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_82826, whole genome shotgun sequence.
ACCESSION   DASSNN010000473 DASSNN010000000
VERSION     DASSNN010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82826"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFL84_08810"
     CDS             <1..788
                     /locus_tag="VFL84_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608761.1"
                     /translation="PIHAHGWDFNDAPLPYLAMLNSGYKDAGVQVSWVVPATLYIQLG
                     AEVLRGDSFPAANGAESSGTGASTAFVHFGGDVGAEASWRAGLSFLTADANDRATDFD
                     SSRVSFTGKSDLAIADFVWKWAKNGNARDRYYVVQAEYLRRDEDGELTLSVPPAGDAT
                     HAYAGTQDGFYVQGVYQFRPRWRVGLRYDRLDASNDLGAVAPTPFVADHDPSRVSVMT
                     DFSNSEFSRLRLQLNRDDSRPETDDQVVVQYIMSLGAHGAHRF"
     gene            802..1704
                     /locus_tag="VFL84_08815"
     CDS             802..1704
                     /locus_tag="VFL84_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HET7608762.1"
                     /translation="MKIILTLTTVVLAAAAAPARAALDVFTCEPEWAALATELGGSLL
                     NASSATTPQQDPHYIQARPSLIAKVRSADLVLCTGADLEVGWLPLLLRQAGNAAVQPG
                     RPGYMTAADQVELLDKPASLDRAAGDIHPYGNPHVQTDPRNIAKVAAALAERLAMLDP
                     ANAAAYRSRHADFAARWQSALERWTAAVAPLRGRQVITHHKGWVYLESWIGLVEVGNL
                     EPKPGLPASAAHLAELLEQVKTQQVAAIIRSTYEDAKASEWLAGRTSIPAVVLPHTVG
                     SVPGADDLFSMFDVIVKTLAEAQQ"
     gene            1701..2486
                     /locus_tag="VFL84_08820"
     CDS             1701..2486
                     /locus_tag="VFL84_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET7608763.1"
                     /translation="MSFDFTGLSIVGPALIAGALVLATHVPLGQEVLRRGIIFIDLAV
                     AQIAGLGVIAAGGLGLEAHGIAAQIAAFASALIGGALLSWTDRRFPKVQEAIIGVSFV
                     LAATGGILLLANNPRGGEELRDLLVGQILWADVHTLAPAAALAALVLASWRFVMRKLG
                     RAGFYLLFAVSVTASVQLVGVYLVFASLMVPALATRSLRAHSLAVGYGVGLVGYVVGL
                     LLSAFFDLPTGAVIVWTLTIAGIALAPLFRRWRGAEAQATQVS"
     gene            complement(2498..>3164)
                     /locus_tag="VFL84_08825"
     CDS             complement(2498..>3164)
                     /locus_tag="VFL84_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HET7608764.1"
                     /translation="LRNGKQTVDFKGLTEQGWIHVPQKYRKYETQGFKTPTGKLELYS
                     TRFEKMGYAPLPYYEEAPETPLSKPDVADEYPFVLTTGGRIPVFFNSEHRQLPLCRKG
                     HPEPLCDIHPQTAAEYGIEDGEWMVIETRRGRIKQRANVTNKIDPRVIHCEHGWWFPE
                     RKGPDYGIWESNANLLTDNRGPFDPQMGTYQLRALLCRVSKLRPEDDHFGPPPLEAET
                     AVA"
BASE COUNT          463 a         1074 c         1082 g          545 t
ORIGIN      
        1 atccgatcca cgcgcacggc tgggacttca acgacgcgcc gctcccgtat ctcgcgatgt
       61 tgaacagcgg ctacaaggac gccggcgtgc aagtgagctg ggtcgtgccg gccacgctgt
      121 acattcagct cggcgcggag gtcttgcgcg gcgactcgtt cccggcagcc aacggcgccg
      181 agagcagcgg caccggagcg tcgacggcct tcgtgcattt cggcggcgac gtcggagccg
      241 aagcgagctg gcgcgccgga ctttcgtttc tgacggcgga tgccaacgac cgagcgacgg
      301 atttcgactc gagccgcgtc tcgttcacgg gaaagagcga cctcgcgatc gcggacttcg
      361 tctggaaatg ggcgaagaac ggcaacgcac gcgaccgcta ttacgtcgtt caggccgaat
      421 atctacggcg cgacgaggac ggcgagctga cgctcagcgt cccgccggcc ggggacgcga
      481 cccacgccta cgccggcacg caagacgggt tctacgtgca gggcgtctat cagtttcggc
      541 cgcgctggcg agtgggcctg cgctacgacc gcctcgacgc gagcaacgat ctcggcgccg
      601 tcgcgccgac gccgttcgtg gccgatcacg atccgagccg cgtcagcgtc atgacggatt
      661 tctcgaacag cgagttcagc cggctgcggc tgcaactgaa ccgcgacgac tcgcgtcccg
      721 agaccgacga tcaggtcgtc gtgcaataca tcatgagcct cggtgcgcac ggcgcgcacc
      781 ggttctgacg gagggcactc catgaaaatc atcttgacgc tcacgacggt cgtgctcgcg
      841 gcggcggccg ctccggcgcg cgcggcgctc gacgtgttca cgtgcgaacc ggaatgggcc
      901 gcgctcgcta ccgagctcgg cggcagcctc ctcaatgcgt cgtccgcgac gacgccgcaa
      961 caggacccgc actacatcca agcgcggccg agcctgatcg cgaaagtccg cagcgcggac
     1021 ttggtgctgt gcacgggagc ggatctcgag gtcggctggt tgccgctgtt gctgcggcaa
     1081 gccggcaacg cggccgtgca gccggggcgg ccgggctaca tgacggccgc ggaccaggtc
     1141 gagctgctcg acaagccggc cagcctcgat cgcgcggccg gcgacatcca cccttacggc
     1201 aacccgcacg tgcagaccga tccgcgcaac atcgccaagg tcgcggccgc gctcgccgag
     1261 cgcctcgcga tgctcgatcc ggcgaacgcc gcggcctatc ggagccgcca cgcggatttc
     1321 gcggcgcgtt ggcaaagcgc gctcgagcgg tggaccgcgg cggtcgcgcc gctgcgcggc
     1381 cgtcaggtca tcacgcatca caaaggctgg gtctacctcg agagctggat cggcttggtc
     1441 gaggtcggca acctcgagcc gaagccgggc ttgccggcga gcgctgcgca tctcgcggaa
     1501 ttgctcgagc aggtgaagac gcagcaagtc gccgcgatca ttcgctcgac ctacgaggac
     1561 gcgaaggctt ccgagtggct cgcgggacgc acgtcgatcc cggccgtcgt gctgccgcac
     1621 acggtcggca gcgtgccggg cgccgatgac ctgttctcga tgttcgacgt gatcgtgaag
     1681 acgctcgcgg aggcgcagca atgagcttcg atttcacggg gctcagcatc gtcgggcccg
     1741 cgctgatcgc cggcgcgctc gttctcgcca cgcacgttcc gctcggtcaa gaggtgctgc
     1801 gccgcggcat catcttcatc gacctggctg tcgcgcagat cgccgggctc ggcgtgatcg
     1861 cggcgggcgg cctcgggctc gaggcgcacg gcatcgctgc ccaaatcgcc gcgttcgctt
     1921 cggcgctgat cggcggcgcg ctcttgtcgt ggaccgaccg gcgctttccg aaagtgcagg
     1981 aagcgatcat cggcgtctcg ttcgtgctgg ccgcgacggg cggcatcctg ctgctcgcga
     2041 acaacccgcg cggcggcgag gagctgcgcg acctcttggt cggccagatc ctgtgggccg
     2101 acgtgcacac gctcgctccc gcggccgcgc tcgcggcgct ggtcctcgcg agctggcgtt
     2161 tcgtgatgcg caagctgggc cgcgcggggt tctatctgct gttcgcggtc agcgtcacgg
     2221 cctcggtgca gctcgtcggc gtctatctcg tcttcgcgag cctcatggtg ccggcgctcg
     2281 cgacgcgctc gttacgcgcg cactcgctcg ccgtcggcta cggcgtcggg ctcgtcggct
     2341 acgtcgtggg gctgctgctg tcggcgttct tcgatttacc cacgggcgcc gtcatcgttt
     2401 ggacgctcac gatcgccggc atcgcgctcg cgccgctgtt tcgccggtgg cgcggcgcgg
     2461 aggcccaagc tacgcaagta agctagcgcg gtcgcggcta ggcgactgcg gtttccgcct
     2521 cgagcggcgg aggcccgaag tgatcatctt cgggccgaag cttcgacact cgacacagca
     2581 gcgcgcgcag ctgatacgtg cccatctgcg gatcgaacgg cccgcggttg tcggtgagca
     2641 ggttcgcgtt cgactcccag atgccgtagt cggggccctt gcgctcgggg aaccaccagc
     2701 cgtgctcgca gtggatcacg cgcggatcga tcttgttcgt gacgttggcc ctctgcttga
     2761 tgcggccgcg gcgcgtctcg atgaccatcc actcgccgtc ctcaatgccg tactcggccg
     2821 ccgtctgcgg gtgaatgtcg catagcggct ccgggtgacc cttgcggcag agcggcagct
     2881 ggcgatgctc cgagttgaag aacaccggta tgcggccgcc ggtcgtcaga acgaacggat
     2941 actcgtcggc cacgtccggc ttgctgagcg gcgtctcggg cgcttcctcg tagtacggca
     3001 gcggcgcgta acccatcttc tcgaaccgcg tcgaatagag ctcgagcttg ccggtcggcg
     3061 tcttgaagcc ttgcgtctcg tattttctgt acttctgcgg gacgtggatc cagccttgct
     3121 ccgtgaggcc cttgaagtcc acggtctgct tgccgttgcg cagc
//
LOCUS       DASSNN010000474         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1466332, whole genome shotgun sequence.
ACCESSION   DASSNN010000474 DASSNN010000000
VERSION     DASSNN010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466332"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..2058
                     /locus_tag="VFL84_08830"
     CDS             217..2058
                     /locus_tag="VFL84_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7608765.1"
                     /translation="MANPSTRDWPASVLIATRLVSLAVVCAALYWGQAVLVPLVLAAL
                     LTILLSPLVTRLDRLRVPRVVGVLLVMALAGGLIGGVGYVVAGQLRSFATELPMHRQN
                     IRAKIRQAIDFTRGGAIEHVQDTIDEINDVVEEDGESAPSARERRRRDDEPLRVTVED
                     PPALLGNAQWLTPLFGAVGTVGLTLLLAIFMLIKREDLRDRLVSLSGRPSLVVATKAF
                     TDAGARITRYLVMQFIVNASMGIAVGIGLYFIGVEYAALWGLAAGVLRYMPYVGPWVA
                     ALLPITVSLVTTPGWEQVAMVVGLFIVLELLSNNVMEPLLYGHSVGLSELAVIVAAIF
                     WTWLWGPIGLVIATPLTACLVVLAAHVPALSAIGRLLGERPALKPHETIYQRLLACDV
                     DEADAIVTRCVSEHSRDEALQVLLDAVLALKRDLQASRVSAEDGELVLSGVRDLLEEL
                     DAAEPPPAAEPAPEHPVLMMGVPGRDPIDAVVLDLARVLLRGEPLVLEVLSTDLMIGE
                     ALTAIEAKAPAAVVVPSLPPAGLAPARQLCMRVHARIPALPLVAARLGDPESELGDRA
                     ALLETAGCAEVATSLGALKSTLGRIARAAARGAPTAGTARAASGKGR"
     gene            2069..>3482
                     /gene="glgX"
                     /locus_tag="VFL84_08835"
     CDS             2069..>3482
                     /gene="glgX"
                     /locus_tag="VFL84_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET7608766.1"
                     /translation="MSEYKLREGMPHPRGAAWDGDGVNFSLFSAHATGVELCLFDANG
                     ERELERIALPEYTDEIWHGYLEGAAPGLVYGYRVQGPYEPEQGHRFNPNKLLMDPYAR
                     ELIGSLTWDPACFGYTLGAENEDLSFDERDSAPFVPKAVVVDPGFDWLKGASPRVPVD
                     RTVIYEMHVRGYTRLHPAVAERHRGTFAGLATPQVIDYVKSLGVTTVELMPIHMFLND
                     WHLLERGLTNYWGYNSIAFFATDPRYLADPLRGVQEFKQMVARFHDAGLEVILDVVYN
                     HTAEGNERGATLSFRGIDNASYYRLLPDQKRYYINDTGTGNTLNLSHPRVIQMVTDSL
                     RYWVQEMHVDGFRFDLGTILAREPNGFDYRSGFLKVCSQDPVLTGVKLIAEPWDCGPG
                     GYQMGEFPPGWGEWNDKFRDTVRDYWRGEAPAAALAPRLYGSADIFDRNGRRPQASVN
                     FVTAHDGFTLNDLVSYNERHN"
BASE COUNT          524 a         1164 c         1198 g          596 t
ORIGIN      
        1 ttcgagcctc ccgactcggt cagtccgcga tttgctgcaa gtgtcatacc gcgcagggcg
       61 tctgcgcgtc gggcccgtgc cgtcgcagga attgcgccgg gacgcaaata ttgcgcatgc
      121 acgcggccgc ggcctgatgc gctcgaaaga cgggccccgc gatggcacgc aacttgctcc
      181 gctgacgcgg gacacggaga gcggcagcag gtgaccgtgg caaacccttc cacgcgcgac
      241 tggcccgcct cggtgctgat cgccacgcgg ctcgtgagcc tcgccgtggt atgtgcggcg
      301 ctctactggg ggcaagccgt gctcgtgccg ttggtcctcg cggcgctgct gacgatcctc
      361 ctgagtcctc tcgtcacccg gctcgatcgt ctgcgcgtgc cgcgcgtcgt cggcgtgttg
      421 ctcgtgatgg cgctcgccgg cggcctcatc ggcggcgtcg gctatgtcgt ggcggggcag
      481 ctgcgcagct tcgcgaccga gcttccgatg caccggcaga acatccgcgc gaagatccgc
      541 caggccatcg atttcacgcg cggcggcgca atcgagcacg tccaggacac gatcgacgag
      601 atcaacgacg tcgtcgagga agacggcgag tccgccccgt cagcgcgcga gcgccggcgc
      661 cgcgacgacg agccgctgcg cgtcaccgtc gaggacccgc cggcgctgct cggcaacgcg
      721 cagtggctca cgccgctgtt cggcgccgtc ggcacggtcg ggctcacgct gctgctcgcg
      781 atcttcatgc tgatcaagcg cgaggacttg cgcgatcgcc tcgtcagtct gtcgggcagg
      841 ccctcgctcg tcgttgcaac gaaagcgttc acggacgccg gcgcgcgaat cactcgctac
      901 ctcgtcatgc agttcatcgt caacgcttcg atgggcattg ccgtcggcat cgggttgtac
      961 ttcatcggcg tggagtacgc cgcgttgtgg ggtctcgcgg ccggcgtgct gcgctacatg
     1021 ccgtacgtcg ggccgtgggt cgccgcgctg ctgccgatca cggtcagcct cgtaacgacg
     1081 ccggggtggg agcaagtcgc gatggtggtc ggcttgttca tcgtgctcga gctcttgagc
     1141 aacaacgtca tggagccgct gctctatggc cacagcgtcg gcttgtcgga gctcgccgtg
     1201 atcgtcgcgg cgatcttctg gacctggctc tggggaccga tcgggctcgt catcgcgacc
     1261 ccgctcacgg cctgtctcgt cgttctcgcg gcgcacgttc ccgcgctctc ggccatcggc
     1321 cgcttgctgg gcgagcggcc ggcgctgaag ccgcacgaga cgatctatca gcggctgctc
     1381 gcatgcgacg tcgacgaggc ggacgcgatc gtcacgcgat gcgtgagcga gcattcgcgc
     1441 gacgaggcgc tgcaggtctt gctcgatgcg gtgctcgcgc tgaagcgcga cctgcaagcg
     1501 agccgcgtat cggccgagga cggcgagctc gtcttgagcg gcgtgcgcga cttgctcgag
     1561 gagcttgacg ccgcggagcc gccgccggcc gccgagccgg ctcccgagca tccggtgctg
     1621 atgatgggcg ttccgggtcg cgacccgatc gacgccgtcg tgctcgacct cgcgcgcgtg
     1681 ctgctgcgcg gcgagccgct cgtgctggag gtgctgtcga cggacttgat gatcggcgaa
     1741 gcgctgactg ccatcgaggc gaaagcgccg gcggccgtcg tcgtgccgtc gctgcctccg
     1801 gccgggctcg cgcccgcccg gcagctctgc atgagggtgc atgcgcgcat accggcgttg
     1861 ccgctcgtcg cggcgcggct cggcgatccc gaatcggagc tcggcgatcg cgccgcgctg
     1921 ctcgagacgg cgggttgcgc ggaggtcgcg acctcgctcg gcgcattgaa gagcactctt
     1981 ggacgcatcg cgcgagccgc agcgcgcggc gcgccgacgg ccggcacggc gcgtgcagcg
     2041 tcgggcaagg gccgctgaaa ggattcgaat gtcggagtac aagcttcgtg agggaatgcc
     2101 gcatccgcgc ggcgcggcgt gggacggcga cggcgtcaac ttctccctgt tctcggcgca
     2161 cgcaacgggc gtggagctgt gcctgttcga cgcgaacggc gagcgcgagc tcgagcgcat
     2221 cgcgttgccg gagtacacgg acgagatctg gcacggttac ctcgagggcg ccgcgccggg
     2281 gctcgtctac ggctatcgcg tccagggccc gtacgagccg gagcagggtc atcggttcaa
     2341 cccgaacaag ctgctcatgg atccgtatgc acgcgagctg atcggcagcc tcacgtggga
     2401 tccggcctgc ttcggttaca cgctcggagc ggagaacgaa gacctctcgt tcgacgagcg
     2461 tgacagcgcg ccgttcgtgc cgaaggccgt cgtcgtcgat cccggttttg attggcttaa
     2521 aggggcgtcg ccgcgcgtcc ccgtggatcg cacggtcatc tacgagatgc acgtgcgcgg
     2581 ctacacgcgt ttgcacccgg ccgtcgcgga acggcatcgc ggcacgttcg cgggcctcgc
     2641 gacgccgcag gtcatcgact acgtcaaatc gctcggcgtg accacggtcg agctcatgcc
     2701 gattcacatg ttcttgaacg actggcacct gctcgagaga ggcctcacga actactgggg
     2761 atacaactcg atcgcgttct tcgcgacgga cccgcgctat ctcgcggatc cgctgcgcgg
     2821 cgtgcaggaa ttcaagcaga tggtcgcgcg gttccacgac gccgggctcg aggtcattct
     2881 cgacgtcgtc tacaaccata cggccgaagg caacgagcgc ggcgccacgc tgtcgtttcg
     2941 cggcatcgac aacgcgtcgt actaccggct cttgccggat cagaagcgct actacatcaa
     3001 cgacaccggc acgggcaata cgctgaacct gagccatccg cgcgtcattc aaatggtgac
     3061 ggacagcctg cgctactggg ttcaggagat gcacgtcgac ggattcaggt tcgatctcgg
     3121 caccatcctc gcgcgcgagc cgaacggctt cgactaccgc agcggattct tgaaagtctg
     3181 ctcgcaggac cccgtcctta ccggcgtgaa gctgatcgcg gagccgtggg actgcgggcc
     3241 aggcggctat cagatgggcg agttcccgcc gggctggggc gagtggaacg acaagtttcg
     3301 cgacacggtg cgcgactact ggcgcggcga agcgccggct gccgccttgg ccccgcggct
     3361 ttacggctcc gccgacatct tcgatcgaaa cggccggcgg ccgcaggcaa gcgtcaattt
     3421 cgtcacggcg cacgacgggt tcacgttgaa cgacctcgtc tcctacaacg agcggcacaa
     3481 cg
//
LOCUS       DASSNN010000475         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_945862, whole genome shotgun sequence.
ACCESSION   DASSNN010000475 DASSNN010000000
VERSION     DASSNN010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945862"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1102)
                     /locus_tag="VFL84_08840"
     CDS             complement(167..1102)
                     /locus_tag="VFL84_08840"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7608767.1"
                     /translation="MAKHKVGWVGAGGRMGFAMAKRLLAAGHEVSVYNRTRSKVEPLA
                     ALGAKIVDSPRALRKHDIVFTTVSASDDLIAVCSGPDGLLDKSGAPKLLIDCSSVSEE
                     ASAQVREAAASLGTEMLAAPVSGNAKVVEAGKLTIVASGPRKSFEMAEPYLQALGVGV
                     TYVGEGELARMVKICHNLLLGIVAQGLAEITVLAEKGGVKRSAFLEFINNSVMGSQFS
                     RYKTPALVNLDWKPTFTPVLLRKDLDLGLKAAKELGAQLPVTKVARGEVDKLVQAGHT
                     ECDFAILLEQAATAAGMKLAPENIAVDDGLKKKSA"
     gene            complement(1113..>2237)
                     /locus_tag="VFL84_08845"
     CDS             complement(1113..>2237)
                     /locus_tag="VFL84_08845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET7608768.1"
                     /translation="GTTVPVGTVLAVVESSMSAAASAPAPAAKAAVAARAAAAAAAPA
                     AASAAPAQLAATGTTTAHVARDDHQRLSPAVRRLAAEYRLDLKAVPGSGRNGRITRRN
                     VLEFIQRQKSAPATAPSAAAAPAARAPSPSPAGPPPAGAKTVPFTRIRKLTAEHMVRS
                     KATSPHVLQAVEVDFQRVDLVRKTVRDAWKAKHGHSLTYLPFIANAVCRALEDFPNLN
                     SSVHGDSLVLHPSVNLGIAVDLGAEGLVAPVIKNADRMSVAQLADAIAEIAQRSRAGK
                     LKPDDFQGGTYTLSNSGTFGTLITAPIINQPQVAILSTDGVRKKPVVVETSEGDSIAI
                     RPVGVLAQSFDHRAVDGAYSAAFLDRVRKIIETSNWVVEV"
BASE COUNT          334 a          747 c          791 g          365 t
ORIGIN      
        1 ggaatacctg acagggccgt ggtcgtggac ttggacctac cagcgcaagc cgggctacaa
       61 gatgtacgag tacgtctgcg aggacaatcg cgagttcgcg gatcccgaaa cgggcgagca
      121 gcgcatgcgc ttcagaaacg aaacggaata gtgaacgcgc gccgcgctac gcggatttct
      181 tcttgaggcc gtcgtcgacg gcgatgttct cgggcgcgag cttcatgccg gccgcggtcg
      241 cggcctgctc gagcagaatc gcgaagtcgc actcggtgtg accggcctgc acgagcttgt
      301 cgacctcgcc gcgcgcgacc ttcgtgacgg gcagctgcgc gccgagctct ttcgcggcct
      361 tgagccccaa gtcgagatcc ttgcgcagca gcacgggcgt gaacgtcggc ttccagtcga
      421 gattcacgag cgccggcgtc ttgtagcgcg agaactgcga gcccatgacg ctgttattga
      481 tgaactcgag gaacgcgctg cgcttcacgc cgcctttctc ggcgagcacg gtgatctcgg
      541 ccagcccctg cgcgacgatg ccgagcagca ggttgtggca gatcttgacc atgcgcgcga
      601 gctcgccttc gccgacgtac gtgacgccga cgccgagagc ctgcagatac ggctccgcca
      661 tctcgaacga cttgcgcggt cccgaggcga cgatggtgag cttgccggcc tcgacgactt
      721 tcgcgttgcc gctgacggga gccgcgagca tctcggtgcc gagcgatgct gcggcttcgc
      781 gcacttgcgc ggacgcctcc tcggacacgc tcgagcaatc gatgaggagc ttcggcgcgc
      841 cgctcttgtc gagcagcccg tcgggacccg agcagaccgc gatcaggtcg tcggaagccg
      901 agacggtcgt gaacacgatg tcgtgcttgc gcagtgcgcg cgggctgtcg acgatcttcg
      961 cgccgagcgc cgcgagcggc tcgaccttgc tgcgcgtgcg gttgtagacg ctgacctcgt
     1021 gtccggccgc gagcagccgc ttcgccatcg cgaagcccat gcggccgcct gcgccgaccc
     1081 agccgacctt gtgcttcgcc atgaacgcta cctcagactt cgacgaccca gttgctcgtc
     1141 tcgatgatct tgcgcacgcg atcgaggaac gccgcggagt aagcgccgtc gacggcgcga
     1201 tggtcgaacg actgcgcgag cacgccgacg ggccggatcg cgatcgagtc gccttcgctc
     1261 gtctcgacta cgacgggctt cttgcgcacg ccgtccgtcg acaggatcgc gacctgcggc
     1321 tgattgatga tcggcgccgt gatcagcgtg ccgaacgtgc ccgagttcga cagcgtgtag
     1381 gtgccgcctt gaaagtcgtc gggcttgagc ttgccggcgc gcgagcgctg cgcgatctcg
     1441 gcaatcgcat cggcgagctg cgcgacgctc atgcggtcgg cgttcttgat gacgggcgcg
     1501 acgaggcctt cggcaccgag atctacagcg atgcctaagt tgacgctcgg gtgcagcacg
     1561 agcgagtcgc cgtgcacgct cgagttcagg ttcggaaagt cctcgagcgc gcggcacacc
     1621 gcgttcgcga tgaacggcaa gtatgtcagc gaatggccgt gcttcgcctt ccacgcatcg
     1681 cgcacggtct tgcgcacgag gtcgacgcgc tggaagtcga cctcgacggc ttgcagcacg
     1741 tgcgggctcg tcgccttcga gcggaccatg tgctcggccg tgagcttgcg gatgcgcgtg
     1801 aacggcacgg ttttcgcgcc ggcgggtggc gggccggctg gcgaaggcga cggcgcgcgc
     1861 gccgccggag cagcggcggc cgagggcgcg gtcgccggcg ccgacttctg gcgctggatg
     1921 aactcgagca cgttgcgccg cgtgatacgg ccattgcgcc cggagcccgg cacggccttc
     1981 aggtcgagcc gatactcggc cgcgagcctg cgcacggccg gcgaaaggcg ttggtggtcg
     2041 tcgcgcgcga cgtgagccgt cgtcgtgccg gtcgccgcga gctgcgcggg cgcggccgac
     2101 gcagcggcgg gcgctgccgc ggcggcggcc gcgcgcgcag ccaccgctgc cttggcggcc
     2161 ggcgccggcg ccgaggccgc tgcgctcatg ctgctctcga cgacggcgag cacggtgccg
     2221 accggcaccg tcgtgcc
//
LOCUS       DASSNN010000476         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_824119, whole genome shotgun sequence.
ACCESSION   DASSNN010000476 DASSNN010000000
VERSION     DASSNN010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824119"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VFL84_08850"
     CDS             complement(<1..872)
                     /locus_tag="VFL84_08850"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HET7608769.1"
                     /translation="MLFNSLTFVVFYLAVFALYWLLRARTPQNVLLLAASWIFYGAWS
                     WKFLLLLLGSTMLDYVCGLLIARAPDRRRKRLVLTVSVTVNLLFLATFKYLGFFVTEF
                     AELLRQLGVDVGLPVLNIVLPVGISFYTFQTIGYVVDVYRGKVPAVRNLLDYALYVAF
                     FPQLVAGPIERAGHMIPQFQTERVWSQPQFESGLQLAVWGLFKKIVLADNLAPYVDAV
                     YDNPQGFSGLTLITATIFFAFQIYCDFSGYTDTARGAARMLGFDLIRNFDFPYISKSP
                     VEFWRRWHISLSQW"
     gene            complement(877..1962)
                     /locus_tag="VFL84_08855"
     CDS             complement(877..1962)
                     /locus_tag="VFL84_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET7608770.1"
                     /translation="MSEPVAVRVPQMNPNDEHAIVVRWHVAKGGRVRAGQTLATLETT
                     KTTFDVDSPSDGYAFFEHEPNAELAVGAPLAWIADNDAPPRIAAPAAAVDSKPASAPA
                     ERFTRKARKLMKELGLGEADFAGVEKVDAEAVERAAAARAPKTPERVPPGAVPLELAP
                     AKIAEIQVLKRVHEQVVPSTVTVALEAERVEARLRREAAEHGPVSALELALYETARLL
                     PEWPELNGFYADGRAWSHATVAIGFAVNLGRSLRVPVVKNAGGASLRDVARAVRDLTL
                     RYMRDELAIEDVTGGTFTITDLSGEGVVNFVPVLNERQSAILGLCAARGDGSRDLVLT
                     FDHRLTDGMRAARFLATLRSRLEETPR"
     gene            complement(1955..>3447)
                     /locus_tag="VFL84_08860"
     CDS             complement(1955..>3447)
                     /locus_tag="VFL84_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7608771.1"
                     /translation="YETLNMAAKWGLPLLVVLENNLYAQSTSQSETLAGDICARAAAF
                     GIATLHGDTWRHEELAALLRAAAAAVRRESKPVFVRVDTYRLAAHSKGDDDRDVREID
                     GYKSRDPLNQFLANGACDQLELAAIRERVERAIERASTAPVLDLAARAKARGSAALSW
                     RQPSPQAGTQLAAINRALAGWLAEDERVLLLGEDVRSPYGGAFKATRGLSDDHPTRVL
                     NTPISEAGIVGIANGLALGGRRPIVEIMFGDFLALCFDQLLNHAAKFRDMYNGRVSSA
                     IVVRTPMGGGRGYGPTHSQNLEKHVVGVPGLRVLVLHGRAPIASLYAALRGSEEPTLI
                     IENKLLYRERADAALPPGYELAETAEPYPTTALRPKGAADVTVVAFGRMSLLAEQVAA
                     ELVGEEVELDLLLPLSVSPFDARPLHESIARTGKLVVVEEGTAHFDLASEVVARAVEG
                     YRGTRCLRVRRVAAQPRPIPSALELELGVLPSVASLREACLELYDE"
BASE COUNT          565 a         1177 c         1191 g          514 t
ORIGIN      
        1 aaccactgcg acaagctgat gtgccagcgc cgccagaact cgacgggcga cttcgagatg
       61 tacggaaagt cgaaattgcg gatcagatcg aagccgagca tgcgcgcggc gccgcgcgcc
      121 gtgtccgtgt agccggagaa gtcgcagtag atctggaacg cgaagaagat tgtcgcggtg
      181 atcagcgtca acccggagaa gccctgcgga ttgtcgtaga cggcgtcgac gtagggcgcg
      241 agattgtcgg cgaggactat cttcttgaac aggccccaca cggcgagctg gagccccgac
      301 tcgaactggg gctggctcca gacccgctcc gtctggaact gcggaatcat gtgccctgcc
      361 cgctcgatcg ggcccgcgac gagctgcggg aagaacgcga catagagcgc gtagtcgagc
      421 aagttgcgca cggccggcac cttgccgcgg tagacgtcga cgacgtagcc gatcgtctgg
      481 aacgtgtaga acgagatccc gaccggcaac acgatgttca ggaccggcag gcctacgtcg
      541 acgccgagtt gccgcagcag ctccgcgaat tcggtgacga agaagccgag gtacttgaac
      601 gtcgccagga acagcaggtt cacggtgacg ctgacggtga gcacgagccg cttgcgccgc
      661 cgatcgggcg cacgcgcgat gagcaggccg cacacgtaat cgagcatggt gctgccgaga
      721 agcagcagca agaatttcca gctccacgcg ccgtagaaga tccagctcgc cgccagcagc
      781 aagacgttct gcggggtccg cgcgcgcagc agccaataca gagcgaagac ggcgagatag
      841 aaaacgacga acgtcagcga gttgaagagc atcggtctag cgcggcgttt cttcgagccg
      901 cgagcgcagc gtcgcgagga accgcgcggc gcgcatgccg tccgtgaggc gatgatcgaa
      961 agtcagcacg agatcgcgcg agccgtcgcc gcgcgccgcg cacagcccga gaatcgccga
     1021 ctgccgctcg ttcagaacgg gcacgaaatt gacgacgccc tcgcccgaga ggtccgtgat
     1081 cgtgaacgtg ccgcccgtga cgtcctcgat cgcgagctcg tcgcgcatat agcgcagcgt
     1141 aagatcgcga acggcgcgcg ccacgtctcg gagcgaggcg ccgcccgcat tcttgacgac
     1201 cggcacgcgc agcgagcggc cgaggttcac tgcgaagccg atcgcgacgg ttgcgtggga
     1261 ccacgcgcgg ccgtcggcat agaagccgtt gagctcgggc cactcaggca acagccgcgc
     1321 cgtctcgtaa agcgcgagct cgagcgccga tacgggaccg tgctcggcgg cctcgcgtcg
     1381 gagcctggcc tcgacgcgct cggcttcgag cgccacggtc acggtgctcg gcacgacttg
     1441 ctcgtgcacg cgcttcagca cctggatctc ggcgatcttc gccggcgcga gctcgagcgg
     1501 aacggcgccc ggcggcacgc gctccggcgt cttcggtgcc cgtgccgccg ccgcgcgctc
     1561 gacggcctcg gcgtccactt tctcgacgcc cgcgaagtcg gcctcgccga ggcccagctc
     1621 cttcatgagc ttgcgcgcct tgcgcgtgaa tcgctcggcg ggcgcgctcg cgggcttcga
     1681 gtcgacggcg gctgccggtg ccgcgattcg cggcggcgcg tcgttgtcgg cgatccacgc
     1741 aagcggcgcg ccgacggcga gctcggcgtt cggctcgtgc tcgaagaatg cgtagccgtc
     1801 gctcggggag tcgacgtcga acgtcgtctt cgtcgtctcg agcgtcgcga gggtttggcc
     1861 cgcgcgaacg cggccgcctt tcgcgacgtg ccaacgaacg acgatggcgt gctcgtcgtt
     1921 gggattcatc tgcggcacgc gcaccgcgac gggctcactc atcgtagagc tccaggcagg
     1981 cttcgcgcag cgatgccacg ctcggcagca cgccgagctc gagctcgagc gcgctcggga
     2041 tcggccgcgg ctgcgccgcg acgcgccgca cgcgcagaca gcgcgtcccg cgatagcctt
     2101 cgacggctcg cgcgacgacc tcgctcgcga gatcgaaatg cgcagtgcct tcctcgacga
     2161 cgacgagctt gcccgttcgc gcgatcgatt cgtgcagcgg ccgcgcgtcg aacggcgaca
     2221 cgctgagcgg caacagcagg tccagctcga cctcctcgcc gacgagctcg gccgcgacct
     2281 gctcggcgag caggctcatg cgcccgaacg cgacgaccgt gacgtcggcc gcgcccttcg
     2341 ggcgcagcgc cgtcgtcgga tacggctccg cggtctccgc gagctcgtac ccgggcggca
     2401 gcgcggcatc tgcgcgctcg cgatagagaa gcttgttctc gatgatcaac gtcggctcct
     2461 cgctgccgcg aagcgcggcg tacagcgacg cgatcggcgc gcggccgtgc agaacgagca
     2521 cgcggaggcc gggcacgccg acgacgtgct tctccaagtt ctggctgtgc gtgggcccgt
     2581 aaccgcgccc tccgcccatc ggcgtgcgga ccacgatcgc gctcgagacg cggccgttgt
     2641 acatgtcgcg aaacttcgcc gcgtggttca ggagctggtc gaagcagagc gcgaggaagt
     2701 cgccgaacat gatctcgacg atcgggcggc ggccgccgag cgcaagcccg ttcgcgatgc
     2761 cgacgatacc ggcttcgctg atcggcgtgt tcagcacgcg cgtggggtgg tcgtccgaca
     2821 gaccgcgcgt cgctttgaac gcgccgccgt acggcgaacg gacgtcctcg ccgagcaaga
     2881 gcacgcgctc gtcttcggcg agccagccgg ccagcgcgcg gttgatcgcc gcgagctgcg
     2941 tgccggcctg cggcgacggt tgccgccacg acagcgcggc cgatccgcgc gctttcgcgc
     3001 gcgccgcaag gtcgagcacg ggcgccgtcg aggcccgttc gatcgcgcgt tcgacgcgct
     3061 cgcgtatcgc ggcgagctcg agttggtcgc aggcgccgtt cgcgaggaac tgattcagcg
     3121 gatcgcggct cttgtatccg tctatttcgc gaacgtcgcg gtcgtcgtcg cctttcgagt
     3181 gggctgcgag ccgatacgtg tcgacgcgca cgaacaccgg cttgctttcc ctccgcacgg
     3241 ccgcggcggc ggcgcgaagc agcgcggcga gctcctcgtg ccgccacgtg tcgccgtgca
     3301 acgtcgcgat gccgaacgcc gccgcgcgcg cgcagatatc tccggcgagc gtctcgctct
     3361 gcgacgtcga ctgcgcgtag aggttgttct cgagcacgac gagcagcggc aagccccatt
     3421 tcgccgccat gttcaaggtc tcgtaca
//
LOCUS       DASSNN010000477         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_586928, whole genome shotgun sequence.
ACCESSION   DASSNN010000477 DASSNN010000000
VERSION     DASSNN010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586928"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFL84_08865"
     CDS             <1..543
                     /locus_tag="VFL84_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7608772.1"
                     /translation="TLNRLEQTPKLVIAALNGHTVGGGLEVALAADIRIARADAGKIG
                     LPEVALGVLPGTGGTQRLARLVGKSRAIELMATGRLLTMAEAQAAGLVNEVWGDEVLA
                     GRSFAEAVHAYARQFTPPAKASRAVGRMKRAVQSGLEAGFAEGLAIERELQQLLFQSD
                     DAREGIAANLEKRKPAFKGQ"
     gene            549..2009
                     /locus_tag="VFL84_08870"
     CDS             549..2009
                     /locus_tag="VFL84_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7608773.1"
                     /translation="MASTTIDQKKLLINNEWRDASGGKTMDVVNPATEEVVATVASAD
                     KNDVDAAVSAARAAFNGPWGKLSARERGRLVSRLADRLMEKADEVSRLETLHNGKPIF
                     ESRQIEIPAAAECFEYYAGWADKVMGETIPVKGNFLTYTLREPLGVVAAIVPWNFPLL
                     LAAWKVAPALATGNTVILKPASQTPLTAIALGEIAVEVGLPPGVLNVVTGPGSSVGQA
                     IVEHPGIDKIAFTGDTSTGKGIMRAAADTLKKITLELGGKSPNVVLPDADIDAALRGA
                     TTGIFYGKGEVCAAGSRLLVAKEIKDEFMTKLAARTKKMVAGDPLDPRTRYGALASKK
                     QLETVSRYVDVAKKEGASLVAGGERIDIGTGKGYFYAPTVFDNVTPEMTIAREEIFGP
                     VLATIEFADLDEAIARANDTQYGLAAAVWTRDVKKAHYVARRLQAGTVWINTYNVYDT
                     AAPFGGYKASGFGREMSAHALEHYTQVKSVWVDLNM"
     gene            2012..>2230
                     /locus_tag="VFL84_08875"
     CDS             2012..>2230
                     /locus_tag="VFL84_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608774.1"
                     /translation="MRITDAHIHIQPFHMMPPAIAATFWTGKPNREELEGFAADPRKL
                     LARMDADGVDRVGLINYVSPDLMGFTADA"
BASE COUNT          416 a          721 c          775 g          318 t
ORIGIN      
        1 acgctgaacc gcctcgagca gacgccgaag ctcgtgatcg cggcgttgaa cgggcacacg
       61 gtcggtggag ggctcgaggt cgcgcttgcc gccgacattc gcattgcgag agccgatgcc
      121 ggaaaaatcg gtctgcccga agtggcgctt ggcgtgctgc cgggcaccgg cggaacgcag
      181 cgcctcgcac ggctcgtcgg caagtcgcgt gcgatcgagc tcatggccac cggcaggttg
      241 ctgacgatgg ccgaagcgca ggcggcaggt ctcgtcaacg aggtctgggg cgacgaggtc
      301 cttgccggcc gctcgttcgc cgaggcggtc cacgcctacg cgcgtcagtt cacgccgccc
      361 gccaaagcga gccgcgccgt cggccgcatg aaacgcgccg tgcagtcggg gctcgaggcg
      421 ggattcgcgg aaggcctcgc gatcgagcgc gaactgcagc agctcctctt tcaaagcgat
      481 gacgccagag agggcatcgc ggccaatctc gaaaaacgca agccggcctt caaaggacaa
      541 taacgagcgt ggcatcgacg acgatcgatc agaagaagct tctcatcaac aacgagtggc
      601 gcgacgcctc cggcggcaag acaatggacg tcgtcaatcc cgcgaccgag gaggtcgttg
      661 cgacggtcgc ctccgccgac aagaacgacg tggacgcggc cgtctccgcc gcgcgggcgg
      721 cgttcaacgg gccctgggga aaactgtcgg cgcgcgagcg cgggcggctc gtgagccgcc
      781 tggcggatcg cctcatggag aaggccgacg aggtctcgcg gctcgaaacg ctgcacaacg
      841 gcaagccgat cttcgaatcg cgtcaaatcg agattcccgc ggccgccgaa tgcttcgagt
      901 actacgccgg ctgggccgac aaggtcatgg gggaaacgat ccccgtgaaa gggaacttcc
      961 tcacctacac gctgcgcgag ccgctcgggg tcgttgccgc gatcgtcccg tggaatttcc
     1021 cgctgctgct ggcggcatgg aaggtcgcgc cggcgctggc gacgggcaac acggtgattc
     1081 tcaaacccgc cagccagacg ccgctgacgg cgattgcgct cggcgagatc gcggtcgagg
     1141 tcgggctgcc gcccggcgtg ctgaacgtcg tgaccggccc cggctcgagt gtcggccagg
     1201 cgatcgtcga gcatcccggg atcgacaaga tcgcgttcac gggcgacacg agcacgggca
     1261 agggcatcat gcgcgcggcg gcggacacgc tcaagaagat cacgctcgag ctcggcggca
     1321 agtccccgaa cgtcgtgctg cctgatgccg acatcgacgc cgcgctgcgc ggcgcgacga
     1381 cggggatctt ctacggcaaa ggcgaagtgt gcgccgccgg ttcgcgcctg ctcgtcgcga
     1441 aggagatcaa ggacgagttc atgacgaagc tcgccgcgcg cacgaagaag atggtcgcgg
     1501 gcgatccgct cgatccccgg acgcgctacg gcgcgctcgc gtcgaagaag cagctggaga
     1561 ccgtcagccg ctacgtggac gtcgcgaaga aggagggggc gtcgctcgtc gcgggcggcg
     1621 agcgcatcga catcgggacg gggaagggtt atttctacgc gccgaccgtg ttcgacaacg
     1681 tcacgcccga gatgacgatc gcgcgcgaag agatcttcgg gcctgtgctc gcgacgatcg
     1741 agttcgcgga cctcgacgag gccatcgcgc gcgcgaacga cacgcaatac ggcctcgccg
     1801 ccgcggtgtg gacgcgcgac gtcaagaagg cgcactacgt cgccaggcgc ctgcaggcgg
     1861 gcacggtctg gatcaacacc tacaacgtgt acgatacggc cgctcccttc ggcgggtaca
     1921 aagcgagcgg tttcggccgc gagatgagcg cccacgcgct cgagcactac acgcaggtga
     1981 agtccgtgtg ggttgatttg aatatgtgag gatgcgcatc accgacgccc acatccacat
     2041 ccagccgttc cacatgatgc cgccggcaat cgcggcaacc ttctggacgg gcaagccgaa
     2101 tcgcgaggag ctggagggct tcgccgcgga tccgcggaag ctgctcgcgc gcatggacgc
     2161 ggacggcgtc gatcgcgtcg gcctcatcaa ctacgtcagc ccggatctca tggggttcac
     2221 cgcggacgcg
//
LOCUS       DASSNN010000478         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_212809, whole genome shotgun sequence.
ACCESSION   DASSNN010000478 DASSNN010000000
VERSION     DASSNN010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212809"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..736)
                     /locus_tag="VFL84_08880"
     CDS             complement(212..736)
                     /locus_tag="VFL84_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7608775.1"
                     /translation="MAICNGGGADDAARAFEALYRRHRDFVLRVARRFTRDRELALDA
                     LQETFAYLLGKFPPAGSGLVLTARLPTLLYPAAKNAAITALRKARRYAGVDAELDELP
                     AETPVEGEPIDAALAALSPERREVLTLRFVDDLSLAEIAAALDVPLGTVKSRLHLALK
                     ELREDSRIKDLFGQ"
     gene            complement(801..1802)
                     /locus_tag="VFL84_08885"
     CDS             complement(801..1802)
                     /locus_tag="VFL84_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HET7608776.1"
                     /translation="MTRAALWLTALAALGCALPADAHRLSPAFFGLTETAPNTFDVQW
                     KVSISGGLAAVLEPRVPDGCSLTEGVRTYVVDDVRLQHGALSCPDGLGGKTFTVNGLA
                     ETQTDVLLRVDYLDGTASSLRLTPSEATVTIPERPSALEVVRTYLVLGVEHILLGYDH
                     LLFVLALLLIVNGVGRLVATVTAFTLAHTITLGAATLGFVHVPSAPVEAVIALSILFL
                     ASELARQGSGGNAAANLMQRFPWVVAFSFGLLHGFGFAGALREVGIPQQAVPLALLNF
                     NVGVEIGQLLFIAAVFGFGWLVRLTTVRVPAIWPRVVAYGIGSIAAFLVVERTIAVF"
     gene            complement(1799..>2206)
                     /locus_tag="VFL84_08890"
     CDS             complement(1799..>2206)
                     /locus_tag="VFL84_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7608777.1"
                     /translation="GARGDALEADAAAGVAALRSGRAPAEVGDRTPLRDSYDDAPREQ
                     VAVLFGDELANALFSARPGEWTGPFRSDFGLHAVRLRGRSEPRLPPYAEIAERVAEEY
                     GAQRRREANEQAYQEMRARYDVVIELPDAEPAQ"
BASE COUNT          368 a          762 c          784 g          292 t
ORIGIN      
        1 gcgagcgccg atcgcgcgaa gcggggcgcc cggcaaagta cgcgcgcgcg gcgtcgagca
       61 ccgcgcgatc ggtgcgcggg tcgacgatcg gctgggcgcg gtcgagcgcg cgcaatcggc
      121 cggcgatcgc gggcggcaag gtctcggtct gttcggtggt cggatccatt ggcgttccta
      181 atgcgttaag cgctgcaacc gcgcaaaagg ttcattggcc gaataagtcc ttgattctcg
      241 agtcctcgcg cagctccttg agagccaggt gcaggcgcga cttgacggtc ccgagcggca
      301 cgtcgagcgc cgccgcgatc tcggcgagcg acagatcgtc gacgaagcgc agcgtcagca
      361 cctcgcgccg ctccggcgat agcgcggcaa gggccgcgtc gatcggctcg ccttctaccg
      421 gcgtttccgc cggcagctcg tcgagctcgg cgtcgacgcc cgcgtagcgg cgcgccttgc
      481 gcaaggccgt gatcgcggcg ttcttcgcgg cgggatagag cagcgtcggg agccgcgccg
      541 tcagcacgag accgcttccg gccggaggga acttgccgag caggtacgcg aacgtttcct
      601 gcagcgcgtc gagcgcgagc tcgcggtcgc gcgtgaaacg ccgcgcgacg cgcagcacga
      661 aatcgcgatg gcggcggtag agcgcttcga acgcgcgcgc cgcgtcgtcc gcgccgccgc
      721 cgttgcagat cgccacgagg tcttcgtcac tgcggcgatc gaccgggccc atgtgcgaag
      781 ctggcccgtg cggctcgcgg ctagaagacc gcgatcgtgc gctcgacgac caagaacgcc
      841 gcaatcgatc cgatgccgta cgcgacgacc ctgggccaga tcgcgggcac gcgaacggtg
      901 gtgagccgca cgagccaacc gaaaccgaac acggcggcga tgaagagcag ctggccgatc
      961 tcgacgccga cgttgaagtt gagcagcgcg agcggcaccg cttgctgcgg gatgccgacc
     1021 tcgcgcagcg ctcccgcgaa gccgaagccg tgcagcaagc cgaacgagaa tgcgacgacc
     1081 cacgggaatc gctgcatcaa gttcgccgcg gcgtttccgc cggaaccctg ccgcgcgagc
     1141 tccgatgcga gaaacagaat gctgagcgcg atgacggcct cgacgggcgc cgaaggcacg
     1201 tgcacgaagc cgagcgtcgc ggcgccgagc gtgatggtgt gcgcgagcgt gaacgcggtc
     1261 acggtcgcga cgagacggcc gacgccgttc acgatcaaca gcagcgcgag cacgaacagc
     1321 agatgatcgt agccgagcag gatgtgctcg accccgagca cgagataggt gcgcacgacc
     1381 tcgagcgcgc taggccgctc agggatcgtg acggtcgcct cgctcggagt caggcgcagg
     1441 ctcgacgccg tgccgtccag ataatcgacg cgcagcagca cgtcggtctg cgtctctgcg
     1501 aggccgttca ccgtgaacgt tttgccgccg agaccgtcgg ggcaggacag cgcgccatgc
     1561 tgaagccgaa cgtcgtcgac gacgtacgtg cgcacgcctt cggtcagcga gcatccgtcc
     1621 ggcacgcgcg gctcgagcac ggccgcgagg ccgccggaga tcgagacctt ccattgcacg
     1681 tcgaacgtgt tcggcgccgt ctcggtgagc ccgaagaacg cggggctcaa gcggtgcgcg
     1741 tcggcgggaa gcgcgcaacc gagcgctgcg agcgcagtca accacagggc agcgcgcgtc
     1801 attgcgccgg ctccgcgtcc ggcagctcga tgacgacgtc gtagcgcgcg cgcatctctt
     1861 gatacgcctg ctcgtttgcc tcgcggcgac gctgcgcgcc gtattcttcc gcgacgcgct
     1921 ccgcgatctc cgcgtacggc ggcaaccgcg gctcgctgcg gccgcgcaag cgaacggcgt
     1981 gcaagccgaa gtccgagcgg aacgggccgg tccattcgcc gggccgcgcc gagaacaggg
     2041 cgttcgcgag ctcgtcgccg aacagcacgg ccacttgctc gcgcggcgcg tcatcgtacg
     2101 aatcgcgcag cggcgtgcgg tcgccgacct cggccggcgc gcgcccggaa cgcagtgccg
     2161 caacacccgc agccgcgtcg gcctcgagcg catcgccgcg cgcacc
//
LOCUS       DASSNN010000479         3192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_316814, whole genome shotgun sequence.
ACCESSION   DASSNN010000479 DASSNN010000000
VERSION     DASSNN010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316814"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VFL84_08895"
     CDS             complement(<1..926)
                     /locus_tag="VFL84_08895"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HET7608778.1"
                     /translation="MRTQGRNRKFLRAAVALSFTVLCAAAQADGDDATIKLTVASSQP
                     TTLAWVGAMHTLVVPELDKRLAARGAPYRIRWTEAYGGSLYKYGETLEGIEVGLADMG
                     WVGTLWELSKLPLQNVTYYAPFASDDFRLVVDIVNELHRTVPAMNDAWTAQNVKFLGA
                     SALDTYHLMTTFPVDSVDDLRGRKILAPGPSAAWLEGTGAVAVDGSLTTYYQQIQTGV
                     ADGVITILTGAAPNRLHEVAPYITLVGLGSQVTGALAINLDTWNRLPDAVREELERLG
                     PEYSRAVADDIAKRYDASIARMRSEGAIVTEFP"
     gene            971..1954
                     /locus_tag="VFL84_08900"
     CDS             971..1954
                     /locus_tag="VFL84_08900"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7608779.1"
                     /translation="MGMLVDGTWHDVWYDTKASQGRFVRSESQFRNWVTRDGSPGPSG
                     RGGFRAEPNRYHLYVAFACPWAHRTLIMRKLKGLESLIDISAVNSYMGPEGWTFAPGP
                     ETIPDSVNGVERLYELYTLADPNYSGRATVPVLWDKQERTIVSNESAEIVRMLNSAFD
                     DVGANSNDYYPKELRVEIDELNAFIYPNINNGVYRAGFATTQEAYEEAAKALFAALER
                     MEGRLATGRFLTGDRFTEADIRLFTTLIRFDPVYNVHFKCNLHRVIDYPNLWGFVRDV
                     YQMPGIADTVHVDFIKRHYYCSHANLNPSRIVPIGPELNYETPHFRERLVH"
     gene            complement(1983..2507)
                     /locus_tag="VFL84_08905"
     CDS             complement(1983..2507)
                     /locus_tag="VFL84_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608780.1"
                     /translation="MKLSIACSLVAGVLATFAIASAAGQSGKNGGNHERAAKVLEADT
                     MVGVSGSLRGATNAIRGVPGGGAPWVLDRAEVELSQDGKLEVEVEGLIIPDRGSNPVA
                     FFRAIVSCLTLDTAGNVTTLNLTTLNGAEVMIGNPLNGDAEIEAQLTLPEPCIAPIVF
                     VTSPTGSWFATTGF"
     gene            complement(2617..>3192)
                     /locus_tag="VFL84_08910"
     CDS             complement(2617..>3192)
                     /locus_tag="VFL84_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HET7608781.1"
                     /translation="GPGSVQWMTAGRGIVHSEFPEQQEGLMWGFQLWVNLPRKDKMTA
                     PRYQDIQGERIPEVELGPGARARVLVGTVNGVAGPVTDVATEPVYFDLHLDPGAQYVA
                     ELPEDHNVFAYVYSGDAKIGAAGTAIERGELALTSAGAKLPVVAGEQGARLIVVGGRP
                     IKEPVARYGPFVMNTEQEIRQAFADYQAGRL"
BASE COUNT          540 a         1087 c         1019 g          546 t
ORIGIN      
        1 gggaactccg tgacgatcgc gccttcgctg cgcatgcgcg cgatgctcgc atcgtaacgc
       61 ttcgcgatgt cgtcggcgac ggcgcggctg tactcgggcc caaggcgctc gagctcttca
      121 cgcaccgcgt cgggcagccg gttccacgtg tcgaggttga tcgcgagcgc gcccgtgacc
      181 tggctgccca acccgacgag cgtgatgtag ggcgcgacct cgtgcagccg gttcggcgcc
      241 gcgcccgtca ggatcgtgat gacgccgtcg gcaacgccgg tttggatctg ctggtaatac
      301 gtcgtcaagc tgccgtcgac ggcgacggcg cccgtgcctt cgagccatgc ggccgacggt
      361 cccggcgcga ggatcttgcg gccgcgcaag tcgtcgacgc tgtcgacggg aaacgtcgtc
      421 atcaagtgat acgtgtcgag cgcgctcgcg cccaggaact tgacgttctg cgccgtccac
      481 gcgtcgttca tcgcgggcac cgtgcgatgc agctcgttga cgatgtcgac gaccaggcga
      541 aagtcgtcgc tcgcgaacgg tgcgtagtac gtcacgttct gcagcggcag cttcgagagc
      601 tcccagagcg tgccgaccca gcccatgtcg gcgagaccga cctcgatgcc ttcgagcgtc
      661 tcgccgtact tgtagagcga gccgccgtac gcttcggtcc agcgaatccg atacggagcg
      721 ccgcgtgccg cgaggcgctt gtcgagctcg ggcacgacga gcgtatgcat cgcgccgacc
      781 caggcgagcg tcgtcggctg gctcgacgcg accgtcagct tgatcgtcgc gtcgtcgccg
      841 tcggcctgcg ccgcggcgca gagcaccgtg aacgaaagag caaccgccgc gcgaaggaac
      901 ttacgattgc ggccctgagt cctcatgaat gcggcaacgg tatcatggga ccttgaaacg
      961 gagatagacg atgggaatgc tcgtcgacgg cacctggcac gacgtctggt acgacacgaa
     1021 ggcgtcgcag ggccggttcg tgcgcagtga atcgcagttc cgcaactggg tcacgcgcga
     1081 cggctcgccg ggtccctcgg gccgcggcgg cttccgcgcc gagccgaacc gctatcactt
     1141 gtatgtcgcg ttcgcgtgcc cgtgggcgca tcgcacgctg atcatgcgca agctgaaagg
     1201 gctcgagtcg ctgatcgaca tttccgccgt caacagctac atgggaccgg agggctggac
     1261 gttcgcgccg ggccccgaaa cgattcccga ttccgtcaac ggcgtcgagc ggctctacga
     1321 gctctacacg ctggccgacc ccaattactc gggccgcgcg acggtgcccg tgctctggga
     1381 caagcaggag cgcacgatcg tgtcgaacga gtcggccgag atcgttcgca tgctgaacag
     1441 cgcgttcgac gacgtcggcg cgaacagcaa cgactactat cccaaggagc tgcgcgtcga
     1501 gatcgacgag ctcaatgctt tcatctatcc gaacatcaac aacggcgtct accgcgccgg
     1561 cttcgcaacg acgcaggaag cgtacgaaga agccgcgaag gcgctattcg cggcgctcga
     1621 gcgcatggaa ggccggctcg caacgggccg gttcttgacg ggcgatcggt tcacggaggc
     1681 cgacattcgg ctgttcacga cgctgattcg gttcgatccg gtctacaacg tgcacttcaa
     1741 gtgcaacctg caccgcgtca tcgactaccc gaacctctgg ggattcgtgc gcgacgtcta
     1801 ccagatgccc ggcatcgcgg acaccgtgca cgtcgacttc atcaagcggc attactactg
     1861 cagccacgcg aacttgaacc cctcgcgcat cgtgccgatc ggccccgagc tcaactacga
     1921 gacgccccac ttccgcgagc ggctcgtgca ctgagcacgc gtttcgtcgc cgcgagagcc
     1981 gctcaaaagc ccgtcgtcgc gaaccacgac cccgttgggg aagtcacgaa cacgatcggc
     2041 gcaatgcacg gctcgggcag cgtgagctgt gcctcgatct cggcgtcgcc gttcagcgga
     2101 ttgccgatca tgacctcggc cccgttcagc gtcgtcagat tcaacgtcgt aacgttgccg
     2161 gccgtgtcca aggtcaagca gctcacgatc gcgcggaaaa acgcgacggg attcgagcct
     2221 cggtccggaa tgatcaggcc ctcgacctcg acctcgagct tgccgtcctg tgagagctct
     2281 acctctgcgc gatccagcac ccacggcgcg ccgccgccgg gcacgccgcg gattgcattc
     2341 gtcgcgccgc gcaacgagcc ggacacgccg accatcgtgt cggcttcgag caccttcgcg
     2401 gcccgttcgt gatttccgcc attttttccg ctctggccgg cagcgcttgc aatcgcgaac
     2461 gttgcgagga ctccggcgac gagtgagcac gcaatcgaaa gtttcatagt ggttttcccc
     2521 tctcggttcc gacctaggag tgagaccact gtgtcccgag ggacgggcgc caagttcatc
     2581 gtgcgagaag gcgccgacgt tccggcgcac tagtcgttag agccggcccg cctgatagtc
     2641 ggcgaacgct tgccgaatct cttgctccgt gttcatgacg aacgggccgt agcgcgcgac
     2701 gggctccttg atcggccgcc cgccgacgac gatgagccgg gcgccttgct ctcccgcgac
     2761 gacgggcagc ttcgcgccgg ccgacgtcag cgcgagctcg ccgcgctcga tcgccgtgcc
     2821 cgcggcgccg atcttggcat cgcccgagta cacgtacgcg aacacgttgt ggtcttcggg
     2881 tagctccgcg acgtactgcg cgcccgggtc caagtgcaag tcgaaataga ccggctccgt
     2941 cgcgacgtcc gtgacgggcc cggcgacgcc gttgacggtg ccgacgagca cgcgtgcgcg
     3001 cgcgcccgga ccgagctcga cctcgggaat gcgctcgccc tggatgtcct gataccgcgg
     3061 cgccgtcatc ttgtccttgc gcggcaggtt cacccacagc tggaagcccc acatgaggcc
     3121 ctcctgctgc tcggggaatt ccgaatgcac gatgccgcgc cccgccgtca tccattgcac
     3181 gctgccgggg cc
//
LOCUS       DASSNN010000480         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1596648, whole genome shotgun sequence.
ACCESSION   DASSNN010000480 DASSNN010000000
VERSION     DASSNN010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596648"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VFL84_08915"
     CDS             complement(<1..895)
                     /locus_tag="VFL84_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET7608782.1"
                     /translation="MSLATRRLTALPQHLILIDPSPARRESMKSAGYFEDAAPREDLH
                     RRSLRGGAISMGALAVNVVVQVCSTIVLARILLPEDFGLVAMIAAVTGAASVFVDLGT
                     RDAVAQRGSITEGEVSALFWITLGTGLALTTITALSAPLIARFYGEPRLGYVTMALSV
                     TFVLPALYFQQHALMRRALMFRTLALIEVGASLLSTAIAIALAYHGYGYWALVWKLLL
                     TPLFTAIAVWISCGWWPKRPTFTPGVKELLKFGLNISGFILADGTARSADRVALGYTA
                     GAQVLGYYQNALGVYEYAIGIC"
     gene            993..2045
                     /locus_tag="VFL84_08920"
     CDS             993..2045
                     /locus_tag="VFL84_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608783.1"
                     /translation="MARPKYKVGDWVEVLGVDEILATLDGDGCLDGMPFMPEMTQFCG
                     RRFQIYKSAHKGCDTVFPVRSRRFDDAVHLSTRCDGSGHAGCQARCLLYWKGAWLKRV
                     EGPAPIPQPDAGGSRRPASAPCRPRMDLARLQQSTIARYEQGVPVFRCQATQLPYAGR
                     EVKWWDVRQYWADYTSGNVGLWTMFRGAVFAAFQTASNLVPGAARPMRRLYDSFRWVW
                     RGAKHPRSTGTIPVGAPTPTARLNLQPGETVRVKSHDEILKTISVASRNRGLWWDAEC
                     VPYCGGTYKVLARVDKIIEDKHGRMVEMKNPCIILDGVVCKARYSDQRMFCPRELYPY
                     WREIWLERVDAPNVPR"
     gene            2070..3071
                     /locus_tag="VFL84_08925"
     CDS             2070..3071
                     /locus_tag="VFL84_08925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7608784.1"
                     /translation="MKDAALSARHARAVTVLTPVYNGADYLPDCIESVLRQSFTDWSY
                     IIVDNKSTDGTLEIARRYAQADDRIRVVANDTFSSMPSNFNRAFALVPGDSRYVKVVC
                     ADDWIMPTCLAELVRHADAHPSAGVVVCHQQSGERVLWRELPAEVGFLRGREACRLGL
                     LEGVHILGSPTASLYRADMIREGPFFPNESPHCDTSACYEKLDRWDFGIVPKVLAVER
                     VHARQISSRIGDVAAANVAYVEVFMKYGRRYLDEPELSARFADVYSDYCKGLGRGLLN
                     LKGKEFWDYQRDELGKLGLRIDYGLVLRGALASLLANAAQPAVAARKLKALLPFLGR"
     gene            3184..>3439
                     /locus_tag="VFL84_08930"
     CDS             3184..>3439
                     /locus_tag="VFL84_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET7608785.1"
                     /translation="MLELSCGGKSIKSALFLGAHSDDIEIGCGGTLQRLVAACPDAHI
                     HWVTLSSDAVRAKETRRAAAKLLEGAKHTIRTEAFRESFFP"
BASE COUNT          624 a         1088 c         1141 g          586 t
ORIGIN      
        1 cgcaaatgcc gatcgcatat tcatagacgc ccaaggcatt ctgatagtag ccgagcactt
       61 gcgctccggc cgtgtagccg agcgcgacgc ggtccgccga tctcgcggta ccgtccgcca
      121 ggatgaagcc gctgatattc aggccgaatt tgagcagctc cttcacgccg ggcgtgaacg
      181 tcgggcgctt cggccaccag ccgcagctga tccacaccgc gatcgcggtg aaaagggggg
      241 taaggagcag tttccagacc agcgcccagt aaccgtagcc gtgataggcg agtgcgatcg
      301 cgatcgctgt cgacaggagg ctcgcgccta cttcgatcaa ggcgagcgtg cggaacatca
      361 gcgcacggcg catcagcgcg tgctgttgga aatacagcgc gggcagcacg aaagtgaccg
      421 acagagccat cgtgacgtat ccgaggcgcg gttcgccgta gaagcgcgcg atcagcggcg
      481 cgctgagggc ggtgatcgtc gtcagggcga gacccgtgcc gagcgtgatc cagaacagcg
      541 cgctgacctc gccctcggtg atgctgccgc gttgcgctac ggcgtcgcga gttccgagat
      601 cgacgaagac cgacgcggcc cccgtcaccg ccgcgatcat cgcgacgagc ccgaagtctt
      661 ctggaagcaa gatgcgcgcg agcacgatcg tcgagcagac ctgcacgacc acgttcacgg
      721 cgagcgcacc catggaaatg gcgccgccac gaagcgagcg gcggtgcaga tcttctcttg
      781 gagcggcgtc ctcgaaataa cctgcggatt tcatcgattc ccggcgagcg ggggaaggat
      841 cgatgagaat gagatgttgc ggcaacgcgg tcaagcgacg cgtggcgagc gacacggctg
      901 cctggctccc tgccgcgcga acgagttgaa ctcgcgactc gttgcgctac attgaccgct
      961 acccttcatt aactgcggcg tgtccttgag cgatggctcg ccccaaatac aaggtcggtg
     1021 actgggtcga agtgctcggc gtcgacgaga ttctggccac tctcgacggc gacggctgtc
     1081 tcgacggcat gccgttcatg cccgagatga cgcagttctg cggacgcagg ttccagatat
     1141 acaagagcgc tcacaaaggc tgcgacacgg tatttccggt ccgctcgcgc cgcttcgacg
     1201 atgcggtgca tctctccacc cgctgcgacg gctcgggcca tgccggttgc caggcgcgct
     1261 gccttttgta ctggaagggg gcgtggctca agcgcgtcga gggtcccgcg ccgattccgc
     1321 agccggatgc cggcgggtcg cggcggccgg catcggcgcc ctgtcgaccg cgcatggatc
     1381 ttgcacgttt gcagcagagc acgatcgcac ggtacgagca gggggtgccg gtctttcgat
     1441 gtcaggcgac gcagcttccc tacgcgggca gggaagtcaa atggtgggac gttcgccagt
     1501 actgggcgga ctacacgtcg ggaaacgtcg ggctctggac gatgttccgg ggcgccgttt
     1561 tcgccgcttt ccaaaccgcc agcaacctcg tccccggcgc cgcgcggccg atgcgccggc
     1621 tctacgattc gtttcgctgg gtttggcgcg gcgccaagca cccgaggagc acgggaacga
     1681 ttcccgtcgg tgcgccgact ccgaccgctc gcttgaatct tcagcccggc gaaacggtcc
     1741 gcgtgaagtc ccacgacgaa atactaaaga ccatcagcgt cgcgagcagg aaccgcgggc
     1801 tgtggtggga cgccgagtgc gttccgtact gcggcggcac ctacaaggtg ctcgcgcgcg
     1861 tcgacaagat catcgaggac aagcacggcc gaatggtgga gatgaagaac ccgtgcatca
     1921 tcttggacgg agtcgtgtgc aaggcgcgtt acagcgacca gcgcatgttc tgcccgcgtg
     1981 agctgtatcc gtactggcgc gagatctggc tcgagcgcgt cgatgcgccg aacgtgccca
     2041 ggtagcgcga gctcgaagca ggggcgcgcg tgaaagacgc cgcattgtcc gcgcgccatg
     2101 ctagagcggt gacggttctc acgccggtct acaacggcgc cgactacttg cccgactgca
     2161 tcgagagcgt gctgcgccaa tccttcacgg attggagcta catcatcgtc gacaacaaga
     2221 gcaccgacgg cacgctcgag atcgcgcgcc ggtacgcgca agccgacgac agaatccgag
     2281 tcgtcgcgaa cgacacgttc tcgagcatgc cgagcaactt caaccgcgcg ttcgccttgg
     2341 tgcccggcga cagccgctac gtcaaggtcg tctgcgcgga cgactggatc atgccgactt
     2401 gcttggccga gctggtccgc catgccgacg cccatccttc ggccggcgtc gtcgtctgcc
     2461 accagcaaag cggcgaacgc gtgctctggc gcgagctgcc ggcggaagtc ggtttccttc
     2521 gcggcaggga ggcttgccga ttgggcctcc tcgaaggagt ccacattctc ggctcgccga
     2581 cggcttctct gtatcgcgcc gacatgatcc gcgaggggcc gttcttcccg aacgaaagcc
     2641 cgcattgcga cacgagcgcc tgctacgaaa agctcgaccg ctgggatttc ggcatcgtgc
     2701 ccaaggtgct cgcggtcgag cgcgtgcacg ctcggcagat cagctcgagg atcggcgacg
     2761 tcgccgccgc gaacgtcgcg tacgtcgagg tgttcatgaa gtacgggcgc cgatatctcg
     2821 acgagccgga gctctcggcg cgtttcgccg acgtctactc cgactactgc aagggattgg
     2881 gccgcggcct gttgaacctg aagggcaagg agttctggga ttaccagcgc gacgagctcg
     2941 ggaagctcgg ccttcggatc gactacgggc tcgttctgcg tggcgccttg gcgtcgcttc
     3001 tcgccaacgc cgcgcagccc gccgtggccg cgcggaagct gaaggcgttg ctgccgttcc
     3061 tcggccgcta gccggcggga atcgtatgcc tgtcgcgaca ccgcggaatg tgcggcgctt
     3121 cagcaagctg cggtaagcgc gtctgttaat ctttgccgca agcaagtcgg cgagaccacg
     3181 taaatgctgg aactgagctg cggcggcaag agcatcaaat cggcgttgtt tctgggcgcg
     3241 cactccgatg acatcgagat cggctgcggc ggcacgctgc agaggctcgt cgccgcgtgc
     3301 cccgacgcgc acattcattg ggtgacgctg agctcggacg ccgttcgcgc gaaggagacg
     3361 cggcgcgccg cggccaagct gttggaaggc gcgaagcata cgatacgcac agaggcgttc
     3421 agagaaagct tcttcccgt
//
LOCUS       DASSNN010000481         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1831058, whole genome shotgun sequence.
ACCESSION   DASSNN010000481 DASSNN010000000
VERSION     DASSNN010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831058"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFL84_08935"
     CDS             complement(<1..512)
                     /locus_tag="VFL84_08935"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET7608786.1"
                     /translation="MTKKHLSRRTVLRGLGVSVALPLLDAMIPARTALAQTAAAAKPK
                     LAFVYFPHGAIMSQWTPAAEGKGFELSPLLAPLAPFKSQLTIVSGLENKHASGPTHAI
                     TPGTWLSGVSPRISHDPYGGVTADQIAAQHIGQDTPLPSLEVSTEEAVGGGACDRNYG
                     CSYGATISFRT"
     gene            complement(630..3068)
                     /locus_tag="VFL84_08940"
     CDS             complement(630..3068)
                     /locus_tag="VFL84_08940"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET7608787.1"
                     /translation="MSRLAAASGIVAVAALGAGIAWLAAHDGAPSAAEQWPLFGQYCI
                     DCHNRDDLTADIAFDRMSADSIAHEPEIFERAVRKLRGAQMPPPGSPQPDRATRRAWI
                     AGLEGELDAAAANARHPGRVALHRLNRTEYANAIAALLDLDVDVKALLPKDDESDGFD
                     NVANVLKVSPSFLEQYIAAARVISEMAVGLPNTKRDSRVYYAEPGINQNYHVAGMPLG
                     TRGGMLVEHFFPADGDYEFDLGGMARARYVEGLDYQHRLILVIDGKKVFEDEIGGPED
                     VEAVDLRQAAAVAEINGRFENIRVPLTAGPHTIAATFVARTMSESDAVLEPFIPGGGE
                     VGIIEGEESPLKIQRLEINGPFTTTGLSDTPSRKRIFTCRPASAAEEMPCAREIVTRL
                     TRQAFRRPVTDADLATPLEFYERGRAAADFEAGIRNALMIVLASPEFLYRFTAPPDDA
                     APGSVYAVDQYGLASRLSFFLWSSGPADELLVLAARGELDDPRVVEDQVKRMLKDPRA
                     AALVTNFAQQWLDIRGIREIVPDPVLFPEYNPDLGKAFAQELTLFLGSVLLEDHSVLE
                     LLGAKHTYLNERLALHYGVPGVRGDEFRRVDLADANRWGLFGKGGILMATSYPNRTAP
                     VLRGAWILESITGTPPASPPPNVEAFPETQEGEQPRTVRERLEMHRSSPSCNGCHGVM
                     DPLGFALENFDAIGAWRVKDREAGAAIDSSGQLADGRVVNGPVDLREALLEDPTQFVQ
                     TLTEKLMIYALGRSVEYYDMPAVRRIVHDAGRENYRFSALLAGIATSEPFRFSTTPPA
                     DDADDAITAQAAEE"
     gene            complement(3068..>3911)
                     /locus_tag="VFL84_08945"
     CDS             complement(3068..>3911)
                     /locus_tag="VFL84_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7608788.1"
                     /translation="QAAAAPTLTDLGSDLVLVEGAGANVVALASADGLLLVDGGSEDR
                     AAALQGALAARWPGRPVTIVFNTNWRAEHTGGNAALRGAGATAMAHENTKLWLGGDFF
                     VDWEDRHYAPHPAAMLPNKTFYTSGKVELGGRNVEYWHLPRAHTDGDVAVFFPDENVL
                     VASDLLSVGRYPVPDYATGGWIGGFIDATQKLLDATDARTRIVAAQGTVYGRAELEAQ
                     LALCKAVAQKTAEAFRVGMSFADFVASKPTAAFDAKWGDPRLFLRLVYKGGFAHLREL
                     GGVI"
BASE COUNT          590 a         1352 c         1347 g          622 t
ORIGIN      
        1 gtgcggaacg agatcgtggc gccgtagctg cagccgtagt tgcgatcgca cgcgcctccg
       61 ccgacggcct cctccgtcga gacctcgagc gacggcagcg gcgtgtcctg gccgatgtgc
      121 tgcgccgcga tttggtcggc cgtgacgccg ccgtacgggt cgtggctgat gcgcgggctg
      181 acgccggaga gccacgttcc gggcgtgatc gcgtgcgtcg gacccgacgc gtgcttgttc
      241 tcgaggccgc tgacgatggt gagctggctc ttgaacggcg cgagcggcgc gagcagcggc
      301 gagagctcga agcccttgcc ctcggccgcg ggcgtccact ggctcatgat cgcgccgtgc
      361 gggaaataca cgaaggcgag cttcggcttc gccgcggccg ccgtctgcgc gagcgccgtc
      421 cgcgccggaa tcatcgcgtc gagcagcggc agcgcgacgc tgactccgag cccgcgtaac
      481 accgtgcggc gcgacagatg tttcttcgtc aagtacatcg caccctccaa aacggtgccg
      541 gtgttttggt tcggtttaac cgaaacaccg gcacttcggt ttaaccgaaa caccggcact
      601 tcggtttaac cgaaccgaaa caccggcacc tactcttcag cggcctgcgc cgtgatggcg
      661 tcgtccgcgt cgtcggcggg cggcgtcgtg ctgaaccgga acggctcgct cgtcgcgatg
      721 ccggcgagaa gcgcagagaa gcggtaattc tcgcggcccg cgtcgtgcac gatgcgccgc
      781 acggccggca tgtcgtagta ctcgacgctg cggccgagcg cgtaaatcat gagcttctcg
      841 gtcagcgttt gcacgaactg cgtaggatct tcgagcagcg cttcacgcaa gtcgacgggc
      901 ccgttcacga ccctgccgtc ggcgagctgg cccgaggagt cgatcgcggc gccggcctcg
      961 cggtctttca cgcgccaagc gccgatcgcg tcgaagttct cgagcgcgaa cccgagcggg
     1021 tccatgacgc cgtggcagcc gttgcagctc gggctcgagc gatgcatctc gagccgctcg
     1081 cggaccgtgc gcggctgctc gccttcttgc gtctcgggga acgcctcgac gttcggcggc
     1141 ggcgacgcgg gcggcgtgcc cgtgatcgac tccagaatcc acgcgccgcg cagcaccggc
     1201 gccgtgcggt tcggatacga cgtcgccatc aagatgccgc ccttgccgaa caagccccag
     1261 cgattcgcgt cggcgagatc gactcgccgg aactcgtcgc cgcgcacgcc cggcacgcca
     1321 tagtgcagcg cgagccgctc gttcaagtac gtgtgcttcg cgcccaagag ctcgagcacg
     1381 ctatggtcct cgagcagcac gctgcccaag aacagcgtga gctcctgcgc gaaggcttta
     1441 cccaaatcgg ggttgtactc cgggaacagc acggggtcgg gcacgatctc gcggatgccg
     1501 cgaatgtcga gccattgctg cgcgaagttc gtgacgagcg ccgcggcgcg cgggtccttc
     1561 agcatgcgct tcacttgatc ttcgacgacg cgcgggtcgt cgagctcgcc gcgcgccgcg
     1621 agcacgagca gctcgtcggc tggcccgctg ctccagagga agaacgacag ccgcgacgcg
     1681 agcccatatt gatcgacggc gtagacggag cccggcgccg catcgtcggg cggcgccgtg
     1741 aagcggtata ggaattccgg cgacgcgagc acgatcatga gcgcattgcg gatgccggcc
     1801 tcgaagtccg cggcggcacg gccccgctcg tagaactcga gcggtgtcgc aagatccgcg
     1861 tccgtgaccg gccggcggaa cgcctggcgc gtcaagcgcg tcacgatctc gcgcgcgcac
     1921 ggcatctctt cggccgcgct cgcagggcgg catgtgaaga tgcgcttgcg gctcggcgtg
     1981 tcgctcaagc ccgtcgtcgt gaacgggccg ttgatctcga gccgctggat cttgagcggg
     2041 ctctcctcgc cctcgatgat gccgacctcg ccgccgcccg ggatgaacgg ctcgagcacg
     2101 gcgtcggact ccgacatcgt gcgcgcgacg aacgtcgccg cgatcgtgtg cgggcccgcc
     2161 gtcagcggca cgcggatgtt ctcgaaccgg ccgttgatct cggcgacggc ggccgcctga
     2221 cgcaaatcga cggcctcgac gtcctcgggt ccgccgatct cgtcctcgaa caccttcttg
     2281 ccgtcgatga cgagaatgag ccggtgctgg taatcgaggc cctcgacgta gcgagcgcgc
     2341 gccataccgc ccaagtcgaa ctcgtaatcg ccgtcggccg ggaagaaatg ctcgacgagc
     2401 atgccgccgc gcgtgccgag cggcatgccg gcgacgtggt agttctgatt gatgccgggc
     2461 tccgcgtagt agacgcggct gtcgcgcttc gtgttcggca agccgaccgc catctcgctg
     2521 atgacgcgcg cggccgcgat gtattgctcg aggaacgacg gcgacacctt cagcacgttc
     2581 gcgacgttgt cgaagccgtc gctctcgtcg tccttcggca gcagcgcctt gacgtccacg
     2641 tcgagatcga gcagcgccgc gatcgcgttc gcatactcgg tacggttcaa gcggtgcagc
     2701 gcgactcgcc cgggatggcg cgcgttcgcg gccgcggcat cgagctcgcc ttcgaggccc
     2761 gcgatccacg cgcgccgcgt cgcgcggtcg ggttgcggcg aaccgggcgg cggcatctgc
     2821 gcgccgcgca gcttgcgcac ggcgcgctcg aaaatctcgg gctcgtgcgc gatcgaatcc
     2881 gcggacatgc gatcgaacgc gatgtcggcg gtcaggtcgt cgcggttgtg gcagtcgatg
     2941 cagtactggc cgaacagcgg ccactgctcc gccgcgctcg gcgccccgtc gtgcgcggcg
     3001 agccacgcga tgccggcgcc gagcgctgcg accgcgacga ttccgcttgc ggcggcgagc
     3061 cggctcatca aatgactccg ccgagctcgc gcaagtgcgc gaacccgcct ttatagacga
     3121 gccgcaagaa cagccgcgga tcgccccact tcgcgtcgaa cgcggcggtc ggcttcgacg
     3181 cgacgaagtc ggcgaagctc atgccgacgc gaaacgcttc ggcggttttc tgcgcgacgg
     3241 ctttgcagag ggccagttgc gcctcgagct cggcgcggcc gtacacggtg ccctgcgccg
     3301 cgacgatgcg cgtgcgcgca tccgtcgcgt cgagcagctt ctgcgtcgcg tcgatgaagc
     3361 cgccgatcca gccgccggtc gcgtaatccg gcacggggta gcgccccact gaaagcaaat
     3421 cgctcgcgac gagcacgttc tcgtcgggaa agaacaccgc gacgtcgccg tccgtgtgcg
     3481 cgcgcggcaa gtgccagtac tcgacgttgc gcccgccgag ctcgactttg cccgaggtat
     3541 agaacgtctt gttcggcagc atcgcggccg gatgcggcgc gtagtgcctg tcttcccagt
     3601 cgacgaagaa gtcgccgccg agccagagct tcgtgttctc gtgcgccatg gcggtcgcgc
     3661 cggcgccccg cagcgcggcg ttgccgcccg tgtgctcggc gcgccagttc gtattgaaca
     3721 cgatcgtgac gggccggccc ggccagcgag ccgcgagcgc gccttgcagc gcggcagctc
     3781 ggtcctcgct gcccccgtcg acgagcagca gaccgtcggc cgaagccagc gcgacgacgt
     3841 tggcgccggc gccctcgacg agcacgaggt ccgacccgag gtcggtcagg gtcggcgcgg
     3901 cggcggcctg c
//
LOCUS       DASSNN010000482         4106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1831333, whole genome shotgun sequence.
ACCESSION   DASSNN010000482 DASSNN010000000
VERSION     DASSNN010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831333"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1239
                     /gene="dnaJ"
                     /locus_tag="VFL84_08950"
     CDS             112..1239
                     /gene="dnaJ"
                     /locus_tag="VFL84_08950"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET7608789.1"
                     /translation="MAKRDYYKVLDVARTATEVEIKKSYRRLAMKYHPDRNPDDHEAE
                     EKFKECKEAYEVLTDAQKRAIYDQHGHEGLAARGGGGFSAADAFSDIFGDVFGDIFGG
                     GRRGGRSQVFRGADLRYDLELTLEQAVAGDSITLDIPAQVECTRCTGKGAEPGSQPVN
                     CKTCGGVGHLRVAQGFFSIQQTCPNCGGAGTTIDKPCRECGGRGRVRKNKTLSVKVPP
                     GVDSGDRIRLAREGEAGRNGGPPGDLYVDITVSPHPIFTREGQNLSCEVPVSFATAAL
                     GGSVDVPTLDGNVVLKVPPETQSGSLFRLRGKGVRSVRESGVGDLFCRVQVETPVNLT
                     AEQKDQLRAFDASIQNEGERHSPRARSWFDGVKEFFERMGA"
     gene            1247..2053
                     /gene="dapB"
                     /locus_tag="VFL84_08955"
     CDS             1247..2053
                     /gene="dapB"
                     /locus_tag="VFL84_08955"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET7608790.1"
                     /translation="MKVAVLGAAGRMGRAVLSCILEADDLTLVGAVTETGDAQLGRDA
                     GELAGGRTAGVPLTDERSHGLHSAQVAVDFTLPTVTEANLRACVEHGAAIVIGTTGLE
                     QRQQKAMEKAAHEIPVVYARNLSVGVNVFMELVARAAKALGDAYDVEIAETHHRHKVD
                     APSGTALALGERIAASRGRRLDELAVYARHGQTGPRVPGTIGFSVARGGNVVGEHVVR
                     FIGAEEDVEFSHRAADRKTFARGALRAARWAAGQAPGLYSMTDVLGLSRG"
     gene            2217..3365
                     /gene="carA"
                     /locus_tag="VFL84_08960"
     CDS             2217..3365
                     /gene="carA"
                     /locus_tag="VFL84_08960"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET7608791.1"
                     /translation="MAEPAILALEDGTVFKGVSVGAEGRTLGEVVFNTAMTGYQEILT
                     DPSYYRQIVTLTYPHIGNVGTNSDDFESTKTYAAGLIIRDLSATVSNWRAEGGLEQFL
                     RRGKTVAIAEIDTRQLTRILRDKGAQAGCIVTGAKAKPEEAVKAARKFPGLKGMDLAK
                     LVTTKQPYQWNQGRIFPERQSVYTKHVAGFHVVAFDYGIKRNILRLIAEQDCRLTVVP
                     ATTKAEEVMALMPDGVFLSNGPGDPEPCDYAIEAIRTLVDKGVPTFGICLGHQLLGLA
                     SGAKTEKMKFGHHGANHPVVELDSGRVMITSQNHGFAVQENTLPNNVRATHRSLFDGS
                     LQGLERTDRPAFSFQGHPEASPGPHDLRPLFQRFTASMKTYRHRAQAV"
     gene            3369..>4106
                     /gene="carB"
                     /locus_tag="VFL84_08965"
     CDS             3369..>4106
                     /gene="carB"
                     /locus_tag="VFL84_08965"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820274.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HET7608792.1"
                     /translation="MPRRDDLESILIIGAGPIIIGQACEFDYSGTQACKALKEEGYRV
                     ILVNSNPATIMTAPASADAIYIEPIHWRAIAEIIAKERPSALLPTMGGQTALNCALDL
                     VREGVLEKYGVELIAASREAIDMAEDRELFRHAMKDIGLATPRAGIAHTMEEAWQVQE
                     EVGFPTIVRPSFTLGGSGGGIAYNRDEFEDIVTRGLDMSPTSEVLIEESVLGWKEFEM
                     EVVRDKNDNCIIVCSIENLDPMGIHTGD"
BASE COUNT          756 a         1337 c         1387 g          626 t
ORIGIN      
        1 aagaacggct aagcttgttt gacgacggac ccactggagc gagatctccc gtgggtccgg
       61 ttttatccgg accacccgag tcaggctgaa gcctgaccac acagacaaca aatggccaag
      121 cgggactact acaaggtgct cgatgtcgcg cggaccgcga ccgaagtcga gatcaagaaa
      181 tcctatcgcc gcctggcgat gaaatatcac ccggatcgca atcccgacga tcacgaggcg
      241 gaagagaaat tcaaggagtg caaagaggcc tacgaggtgc tgaccgacgc gcagaagcgc
      301 gcgatctacg atcagcatgg ccacgaggga ctcgctgcgc gcggcggcgg cggattcagc
      361 gcagccgacg ccttcagcga catcttcggc gacgtgttcg gcgacatctt cggcggcggg
      421 cggcgcggcg gccgcagcca ggtattccgc ggcgcggatc tgcgttacga cctcgagctg
      481 acgctcgagc aggccgtcgc cggcgattcg atcacgctcg atatcccggc ccaggtcgag
      541 tgcacgcgct gcacgggcaa gggagcagag cccggctcgc agcccgtcaa ctgcaagacc
      601 tgcggcggcg tcggccatct gcgcgtggcc cagggcttct tctcgattca gcagacctgc
      661 ccgaattgcg gcggcgccgg cacgacgatc gacaagccgt gccgcgagtg cggcggccgc
      721 ggccgcgtgc gcaagaacaa gacgctgtcc gtcaaagtgc cgcccggcgt cgacagcggc
      781 gatcggatcc gcctcgcgcg cgagggcgag gccgggcgga acggcgggcc gccgggcgat
      841 ctctacgtcg acatcacggt gtcgccgcat ccgatcttca ctcgcgaggg ccagaacttg
      901 agctgcgagg tgccggtcag ctttgcgacg gcggctctcg gcggcagcgt cgacgtgccg
      961 acgctcgacg gcaacgtcgt tctcaaggtt ccgcccgaaa cccagtcggg gagtctgttc
     1021 cggctgcggg ggaagggagt acgctccgtc cgcgagagcg gcgtcggcga cctcttctgc
     1081 cgcgtgcagg tcgagacgcc cgtgaatctg acggccgagc agaaggacca gctccgcgct
     1141 ttcgatgcgt cgatccagaa cgagggcgag cggcacagcc cgcgagcgcg gtcctggttc
     1201 gacggcgtca aagagttttt cgagcgaatg ggagcatagt ccgttgttga aggtagccgt
     1261 tcttggcgcc gcgggtcgca tgggccgcgc ggtgttgagc tgcatcctcg aggccgacga
     1321 tctgacgctc gtcggcgcgg tcaccgagac cggcgacgcg cagcttggcc gcgacgccgg
     1381 cgagctcgcg ggcggccgca cggccggcgt gccgctgacc gacgagcgca gccacggcct
     1441 gcactccgct caggtggccg tcgatttcac gctgccgacc gtcaccgagg cgaaccttcg
     1501 ggcctgcgtc gagcacggcg ccgcgatcgt gatcggcacg acggggctcg agcagcgcca
     1561 gcagaaggca atggagaagg ccgcgcacga aatcccggtc gtctacgccc gcaacctgag
     1621 cgtcggcgtc aacgtgttca tggagctcgt tgcgcgcgcc gcgaaggcgc tcggcgacgc
     1681 ctacgacgtg gagatcgcgg agacgcacca ccggcacaag gtcgacgcgc cttccggcac
     1741 ggcgctcgct ctcggggaac gcatcgccgc cagtcgcggt cgtaggctcg acgagttggc
     1801 cgtctacgcg cgccacggcc agacggggcc acgggtgccg ggcacgatcg gcttctcggt
     1861 cgctcgcggc ggcaacgtcg tcggcgagca cgtcgtgcgt ttcatcggcg ccgaggaaga
     1921 cgtggagttc tcgcaccggg ccgccgaccg caagacgttc gctcgcggcg cgctgcgcgc
     1981 ggcgcgctgg gccgccggtc aggcgccggg cctgtactcg atgacggacg tgctcggttt
     2041 gtcccgcggc tgagcgccgc gaatttttcc gcaggctcgc aaacggctag aacgttctca
     2101 aaaggacggg ctacaattgc cgcctgagtt tccccgttga tgaacacggg attggctgcc
     2161 gggcttgcag cccatcccgc gtcttttcgc aagccgaatt cttgtaggag ctcagcgtgg
     2221 cagagccggc gatactagcg ctcgaagatg ggacggtgtt caagggtgtg tcggtcggcg
     2281 ccgaaggccg cacgctcggc gaagtcgttt tcaacaccgc catgacgggc tatcaggaga
     2341 tcctgacgga cccgtcgtac taccgtcaga tcgtgacgct gacgtatccg cacatcggca
     2401 acgtcggcac caactccgac gacttcgagt cgacgaagac ctacgccgcc ggcctcatca
     2461 tccgcgattt gtcggcgacg gtcagcaact ggcgcgccga gggcggtctc gagcagttcc
     2521 tgcggcgcgg caagaccgtc gcgatcgccg agatcgacac gcggcaattg actcgcattt
     2581 tgcgcgacaa gggcgcgcaa gccggctgca tcgtcacggg cgcgaaggcc aagccggaag
     2641 aggccgtcaa agccgcgcgc aagtttccgg gcctgaaagg catggatctc gcgaagctcg
     2701 tcacgacgaa gcagccctac cagtggaacc aagggcgcat cttccccgag cggcagagcg
     2761 tctacacgaa gcacgtcgcc ggcttccacg tcgtcgcgtt cgactacggc atcaaacgca
     2821 acatcttgcg cttgatcgcg gagcaggatt gccggctcac ggtcgtccca gcgacaacca
     2881 aagccgagga ggtcatggcg ctgatgccgg acggcgtatt cctgtcgaac ggacccggcg
     2941 atcccgagcc gtgcgattac gcgatcgaag ccatccgcac gctcgtcgac aaaggcgtgc
     3001 cgacgttcgg catctgcttg ggccaccagc tgctcggtct cgcgagcggc gccaagaccg
     3061 agaagatgaa gttcggccac cacggtgcga accaccccgt cgtcgagctc gactcgggac
     3121 gcgtcatgat cacgagccag aaccacggct tcgccgtgca agagaacacg ttgccgaaca
     3181 acgtgcgcgc gacgcatcgc tcgctgttcg acggcagctt gcaaggcctc gagcggaccg
     3241 accggccggc gttctcgttc caaggccacc ccgaggcgag cccggggccg cacgacttga
     3301 ggccgctgtt ccaacgcttc acggcctcca tgaaaacgta tcgccatcgt gctcaagcag
     3361 tctgaacaat gccgcgtcgc gacgatctcg aaagcatcct gatcatcggc gccggcccga
     3421 tcatcatcgg gcaggcgtgc gagttcgatt actcgggcac gcaggcctgc aaggcgctca
     3481 aggaagaggg ttatcgagtc attctcgtga actcgaatcc ggcgacgatc atgaccgccc
     3541 cggcgagcgc cgacgcgata tacatcgagc cgattcactg gcgcgcgatc gccgagatca
     3601 tcgcgaaaga gcgcccgagc gcattgctgc cgacgatggg cggccagacc gcgctcaact
     3661 gcgcgctcga tctcgtgcgc gagggcgtgc tcgagaagta cggcgtcgag ctcatcgcgg
     3721 cgtcgcgcga ggccatcgac atggccgagg accgcgagct gtttcgtcac gcgatgaagg
     3781 acatcgggct cgcgacgccg cgggccggca tcgcgcacac gatggaggaa gcgtggcagg
     3841 tgcaggagga ggtcgggttc ccgacgatcg tgcggccgtc gttcacgctc ggcggcagcg
     3901 gcggcggcat cgcgtacaac cgcgacgagt tcgaggacat cgtcacgcgc gggctcgaca
     3961 tgtcgccgac gagcgaagtg ctgatcgaag agtcggtgct cggctggaaa gagttcgaga
     4021 tggaggtcgt tcgcgacaag aacgacaact gcatcatcgt ctgctcgatc gagaatctcg
     4081 atccgatggg catccacacg ggcgac
//
LOCUS       DASSNN010000483         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1096271, whole genome shotgun sequence.
ACCESSION   DASSNN010000483 DASSNN010000000
VERSION     DASSNN010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096271"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /locus_tag="VFL84_08970"
     CDS             complement(<1..1513)
                     /locus_tag="VFL84_08970"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HET7608793.1"
                     /translation="MRLRWVGWCALSCAGWASAQETAPLVDPGQKTVRIERTTERPVI
                     DGALDDAVWASAARIDDLHQVNPVEYAEPSERTEIYLLYDDDALYIGARMFTPPGEIT
                     ANVMRQGASITQEDTLFVTLDPFNTQRGGYFFGLNAHGVRFDGLYRNVSEYYSDWDSI
                     WFAGARRFDGGWTAEYEIPFKSLSFDPGSDTWGLNFSRSLETRNEDMAWVSRNRRWDP
                     SSAGLMTGLTALDQGIGLDVVPSASLVNRRVLRNDDPSTPVVELDPDTSDDDFEPSLD
                     VFYKLTPQMNAALTINTDFSATEVDDRQVNLTRFGLFFPEKRDFFLREADIFEFGRIG
                     ASDGTGALSNAERQNARPFFSRRIGLGAFGEPVDLNYGGKVSGRAGRFELGALSIRQD
                     ENPLAGVDDTTLSVVRAKAGIGSESTVGAVFTNGDPLNPRDNSLVGLDALYRNSRLPG
                     GRTIEAGVWYEETETEALPNETRPAGGNSAAGLGISVPSNNKLRGAFSTRRVEA"
     gene            complement(1642..>2825)
                     /locus_tag="VFL84_08975"
     CDS             complement(1642..>2825)
                     /locus_tag="VFL84_08975"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HET7608794.1"
                     /translation="VRRFHPLIALAALALLPAAHAHEVPSDVTVRMLVRPDGDRVRLL
                     VRAPLEAMQDIEFPTLGPGYLDVPRAAPQLRAAAQVWLLDNLELYEGERPLPLTIVAV
                     RASIPSDRSFADFDAALAHLHAEPLPAGLDLPWQQALLDVELAAPIESESSLFSLRAR
                     TERLGRRVVNAVRFFGASGVERVFQLEGDAGVVALDPRWHQSMLRFLAQGFEHILDGA
                     DHLLFLLCLVVPFRRQLRALVSIVTAFTAGHSVTLIASAYGAAPSALWFPPLVETLIA
                     VSIVYMCVENVVLPTPKTRWPIAFAFGLVHGFGFSFALQNTLQFAGSHVLTSLLSFNV
                     GIELGQLLVLVLMVPGLNLLFRYVPERAGGIVIAVLVGHTAWHWLLERFAALRAFDLW
                     P"
BASE COUNT          509 a          951 c          958 g          407 t
ORIGIN      
        1 tcgcctcgac gcgccgcgtc gagaatgcgc cgcggagctt gttgttgctc ggcaccgaga
       61 tgccgagacc cgccgcggag ttgccgccgg cgggccgcgt ctcgttgggc agcgcctcgg
      121 tctcggtctc ctcataccag acgccggcct cgatcgtgcg gccgcccgga agccgcgagt
      181 tgcgatagag cgcgtctaga cctacgagcg agttgtcgcg cgggttcaat gggtcgccgt
      241 tcgtgaacac cgcgccgacc gtcgactcgc tgccgatgcc ggccttcgcg cgcacgacgg
      301 acagcgtcgt gtcgtcgaca cccgcgagcg gattctcgtc ctgacgaatc gacaatgcgc
      361 cgagctcgaa gcggccggcg cggccgctca ccttgccgcc gtagttcaag tcgacgggct
      421 cgccgaaggc gcccaagccg atgcgccgcg agaagaacgg gcgcgcgttc tgccgctcgg
      481 cgttgctgag cgctcccgtc ccgtccgacg cgccgatgcg gccgaactcg aagatatcgg
      541 cctcgcgcaa gaagaagtcg cgcttctccg ggaagaacaa gccgaaccga gtcagattca
      601 cttggcggtc gtcgacttcc gttgccgaga agtccgtgtt gatcgtcaac gccgcattca
      661 tctgcggcgt cagcttgtag aagacgtcga gcgacggctc gaagtcgtcg tccgacgtgt
      721 cgggatcgag ctcgacgacc ggcgtcgaag gatcgtcgtt gcgcagcacg cgccggttca
      781 cgaggctcgc cgacggcacg acgtcgaggc cgatgccctg gtcgagggcc gtgagccccg
      841 tcatgagccc cgcgctgctc gggtcccagc gccgattgcg cgagacccac gccatgtcct
      901 cgttgcgggt ctcgaggctg cgcgagaagt tgaggcccca cgtgtcgctc cccggatcga
      961 acgacaacga tttgaacggg atctcgtact cggccgtcca gccgccgtcg aagcgccgcg
     1021 cgcctgcgaa ccagatgctg tcccagtcgc tgtagtactc ggagacgttg cgatagagcc
     1081 cgtcgaagcg cacgccgtgc gcgttcaagc cgaagaagta accgccgcgc tgcgtgttga
     1141 acggatcgag cgtgacgaac agcgtgtctt cctgcgtgat cgaagcgcct tggcgcatga
     1201 cgttcgcggt aatctcgccg ggcggcgtga acatacgcgc gccgatgtag agcgcgtcgt
     1261 cgtcgtagag cagatagatc tccgtgcgct cggacggctc ggcgtattcg acgggattca
     1321 cctggtgaag atcgtcgatg cgcgcggcgc tcgcccacac ggcgtcgtcg agcgcgccgt
     1381 cgatgacggg ccgctccgtc gtgcgctcga tgcgcacggt cttctgtccg ggatccacga
     1441 gcggcgcggt ctcctgggcg gacgcccatc ctgcgcacga cagcgcgcac caccctaccc
     1501 accgcaaccg catctatttg tgatcgccga ctcaggaaag cgcgaacgat aaccgaaaca
     1561 cgctctcggc ggcgcggcga aatcggcgcg aagccgagga caagcgccac ctttggaaag
     1621 cgctgctcgg tttgcgctcg atcaaggcca gagatcgaaa gcgcgcagcg ccgcgaagcg
     1681 ctcgagcaac cagtgccagg ccgtatgccc gacgagcacg gcgatcacga tgccgcccgc
     1741 gcgctcgggc acgtagcgga acagcaggtt cagccccggc accatgagca cgagaacgag
     1801 cagttggccg agctcgatgc cgacattgaa cgagagcagc gacgtcagca cgtggctgcc
     1861 ggcgaactgc agcgtgttct gcaatgcgaa cgagaagccg aagccgtgca cgaggccgaa
     1921 ggcgaacgcg atcggccaac gcgtcttcgg cgtcggcaac acgacgtttt cgacgcacat
     1981 gtagacgatc gacactgcga tcagcgtctc gacgagcggc ggaaaccaca gcgcggacgg
     2041 tgccgcaccg taggccgagg cgatcagcgt cacggaatgg cccgccgtga acgccgtgac
     2101 gattgagacg agcgcgcgca gctgacgccg aaacggcacg acgaggcaga gcaggaacaa
     2161 caggtgatcg gcgccgtcga gaatgtgctc gaagccttgc gcgaggaacc gcagcatgga
     2221 ttgatgccag cgcggatcga gcgcgacgac tccggcgtcg ccttcgagct ggaacacgcg
     2281 ctcgacgccg ctcgcgccga agaaacgcac ggcgttcacg acgcgtcggc cgagccgctc
     2341 cgtgcgcgcg cgcaacgaga acagcgagct ctcggactcg atcggcgccg cgagctctac
     2401 gtcgagcaac gcttgctgcc acggcagatc gagcccggcc ggcagcggct cggcatgcaa
     2461 gtgagcgagc gccgcgtcga agtcggcgaa ggaccggtcg cttgggatcg atgcgcgcac
     2521 cgcgacgatc gtgagcggca acggccgctc gccttcgtag agctcgaggt tgtcgagcag
     2581 ccagacctgc gccgcggcgc gcagctgcgg cgcggcgcgc ggaacgtcga gatagccggg
     2641 cccgagcgtc ggaaactcga tgtcttgcat cgcctcgagc ggcgcccgca ccaggagccg
     2701 cacgcggtcg ccgtccggcc gcacgagcat gcgcaccgtg acgtcgctcg gaacctcgtg
     2761 cgcgtgcgcc gcgggcagca gcgccagcgc cgcgagcgca atcaatggat ggaaccgccg
     2821 caccc
//
LOCUS       DASSNN010000484         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_317811, whole genome shotgun sequence.
ACCESSION   DASSNN010000484 DASSNN010000000
VERSION     DASSNN010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317811"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..734)
                     /locus_tag="VFL84_08980"
     CDS             complement(165..734)
                     /locus_tag="VFL84_08980"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HET7608795.1"
                     /translation="MRVTATATLLMTAAFTAWVGPVLADPPAHAPAHGWRKKHDPYYV
                     GYTGVSWERDYEISSGRCNREEIATVVGGIVGGAIANRVADEHKAVATIVGVIAGATI
                     GNRIGKELDEADRGCFGHALEIAQPGQRITWANPSAGLSYEMSPGAGSTRNGAKCREY
                     TLVTIAGRQRSTQTGVACQSQPGVWQTRS"
     gene            1018..1536
                     /locus_tag="VFL84_08985"
     CDS             1018..1536
                     /locus_tag="VFL84_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608796.1"
                     /translation="MARVIRAALLAMGFAFVAGAATAQQPATAEMRSLDEQVQEIKTD
                     VLGIAAELNRLEEKLLYPSNTQLAVFVSIAEGETFRLDSMQINIDGQLATHFLYSFKE
                     LDALQHGGVQRIYTGNVPTGSHQIDVSINGKTPGGDDFVSSESFSFDKGIEPKLLGIA
                     LNGPKSIELGEW"
     gene            1549..>2324
                     /locus_tag="VFL84_08990"
     CDS             1549..>2324
                     /locus_tag="VFL84_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7608797.1"
                     /translation="MRVHAVVISLLIGLPAAVQADAPAPESTTRDWYFGEAIYYAQQG
                     HYFEALERLDAELGQHRAVDEPALDSLYRNLHEAEFDIGDFELNYRMHHRAGRAITAV
                     LEGAVDEEVRNDAAYRLARIHFQKGQLEDALIALDRITGRVPERIVDDVAFLRANTLM
                     GLGRQAEAADVLKRLQNAEGLKGFAAYNLGVALLEDNRRQDALEQLDRAGQIQSSDEA
                     VLAIRDKSNFVLGTLLVEDQQFARAKQAFDRVRLEGPFSN"
BASE COUNT          423 a          756 c          756 g          389 t
ORIGIN      
        1 agcggctatc ggctcgtgga cttcgtcgac gacgacagcg gaggcggaga tgccgatgca
       61 gacgcgtcac gtgacgcgtt ttgcgccacg gcagcgcaaa gcgacgtcga acgagccgaa
      121 gtacgcgaac cgcgcggcgt ctaccgccta acgcgaccgc cggcctacga gcgcgtctgc
      181 cagacgccgg gctgagactg gcacgcgacg ccggtttgcg tcgagcgctg ccggccagcg
      241 atcgtgacga gcgtgtattc gcggcacttc gcgccgttac gcgtgcttcc cgcgcccggc
      301 gacatctcgt agctcaagcc ggccgaggga ttcgcccagg tgatgcgttg gcccggctgc
      361 gcgatctcca gcgcatggcc gaagcaaccg cgatcggctt cgtcgagctc tttgccgatg
      421 cggttgccga tcgtggcacc ggcgatgacg ccgacaatcg tcgcgacggc tttgtgctcg
      481 tccgcgacgc ggttcgcgat cgcaccgccg acgatgccgc cgacgacggt cgcgatctcc
      541 tcgcgattgc agcggcccga cgagatctcg tagtcgcgct cccacgaaac acccgtgtag
      601 ccgacgtagt acggatcgtg cttctttcgc cagccgtgcg cgggcgcgtg cgccggcggg
      661 tccgccaaga ccgggcctac ccacgccgtg aaagcggccg tcatcaatag agtggctgtc
      721 gcggtgacgc gcatatgagt gctcctccct gaggcgccag attgtggacc gcgatcgcgc
      781 cgcaaaatga gagccgggtc acaagcggag atcgtttcgc ttgccatgaa atggccgcaa
      841 acgtgtccgc ccggggcgac agtcttcgac aattttacgg tgcacccgcg gaaatgctgt
      901 cggcaaagca cgactgccct ttttgcgagc ccttttttgc ggtccagttc acatagcgcg
      961 gcacccgctt caaagacact tctgtcgatc ttcaagacgc agggaagggt tttgacgatg
     1021 gcgagggtaa ttcgcgccgc actgctcgcg atggggtttg cgttcgtggc cggcgcagcg
     1081 actgcgcaac agccagcgac tgccgagatg cgcagcctcg acgagcaagt gcaggaaata
     1141 aagaccgacg tgctcgggat cgcggcggag ctgaatcgcc tcgaggaaaa gctgctctat
     1201 ccctcgaata cgcagctcgc cgtgttcgta tcgatcgcgg aaggcgagac gttccgcctc
     1261 gactcgatgc agatcaacat cgacggccag ctcgcgacac acttcctcta cagcttcaaa
     1321 gagctcgacg cgctgcagca tggcggcgtg caacgcatct acacgggcaa cgtgccgacg
     1381 ggcagccatc agatcgacgt ttcgatcaac ggcaagacgc ccggcggcga cgatttcgtg
     1441 agctccgaat cgttctcgtt cgacaagggc atcgagccga agctgctcgg cattgcgctc
     1501 aatggcccca agtcgatcga gctcggcgag tggtgagcgg cgtttgccat gcgcgtccac
     1561 gcggtcgtca tctcgctatt gatcggcctg ccggccgcgg tgcaggccga tgcgcctgcg
     1621 ccggagtcga ccacgcgtga ctggtatttc ggcgaagcga tctactacgc gcagcagggg
     1681 cattacttcg aagcgctaga gcggctcgac gccgagctag gccagcaccg cgccgtcgac
     1741 gagcccgcgc tcgactcgct ataccgcaac ctgcacgagg ccgagttcga catcggcgac
     1801 ttcgagctca actaccgaat gcaccaccgc gccggccgcg cgatcacggc cgtgctcgag
     1861 ggcgccgtcg acgaagaggt gcgtaacgat gccgcgtatc ggctcgcacg gattcatttc
     1921 cagaaggggc agctcgagga cgcgctgatt gcgctcgacc gcatcacggg gcgcgtgccc
     1981 gaaaggatcg tcgacgacgt cgctttcttg cgcgcgaaca cgctgatggg gctcggccgc
     2041 caggccgaag cggcagacgt cctgaagcgc ctccagaacg ccgaggggct caagggcttc
     2101 gcggcctata acttgggcgt cgcgttgctc gaggacaatc ggcggcagga cgcgctcgag
     2161 cagctcgatc gcgccgggca gatccagagc agcgacgaag ccgtgctcgc gatccgcgac
     2221 aagtcgaact tcgtgcttgg cacgttgctc gtcgaggacc agcagttcgc gcgcgcgaag
     2281 caggcgttcg accgcgtccg gctcgaaggc ccgttctcga acag
//
LOCUS       DASSNN010000485         4721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_947433, whole genome shotgun sequence.
ACCESSION   DASSNN010000485 DASSNN010000000
VERSION     DASSNN010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947433"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..2592)
                     /gene="rlmKL"
                     /locus_tag="VFL84_08995"
     CDS             complement(361..2592)
                     /gene="rlmKL"
                     /locus_tag="VFL84_08995"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HET7608798.1"
                     /translation="MHSLYVTAPAGTADLAADELAACGLADLKVERGGVACSGSLEQA
                     YRACLWSRVANRVLLAVAKFSAPTPEALYDGVRTVDWSEHLSVDGTLAVEATSTRSSV
                     AHTQFAALKTKDAIVDQFRERFGARPSVDVAAPDVRINLHLDRDVATLAIDLSGESLH
                     RRGYRGPQGAAPLKENLGAAVLLRAGWQKLASAADGGELGFVDPMCGSGSLAIEAALI
                     AGDVAPGLLRNEFGFLRWRGHDEAAWQRLLTEAAERRAAARLDRFVFRAYDRDAAAVR
                     ATLENATRAGFAKHVHAERRELEDLPAAPAPRGLVAVNPPYGERLGDEDALKKTYALL
                     GEKLKAGYVGWQAAVLTGNPPLGRELRIRAKRTHTMYNGPIECRLLRFDIEPKHFEEK
                     RVPGALPAADAAARARPGAEMFANRLRKNVASLGEWAREEGVACYRVYDADMPEYAFS
                     IDVYAASAEPSARRFAYVQEYAPPKTVDAEKARARRAEAFSVLPEVLEVPRERVYVRT
                     RRKQKRGAQYEKLAERGEFEVVEEGGLKFLVNFTDYLDTGLFLDHRPTRARIRELAAG
                     KTFLNLFAYTATATVFAAAGGARATTSVDLSRTYLDWAQRNLERNGFDDANRHRLIQA
                     DVLEWLEQRERERYDLIFLDPPTLSRSKRMAKELDVQRDHVELIRSTLLKLAPSGLLI
                     FSTNFRKFRLDEAGLAGLAVADVTPATIPKDFARNPRIHRCFEIRVSQGAAKAPRPVL
                     SLR"
     gene            complement(2664..3491)
                     /locus_tag="VFL84_09000"
     CDS             complement(2664..3491)
                     /locus_tag="VFL84_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608799.1"
                     /translation="MVTGKTELLRAQRRWADAHGVRYDARGFVRALADNLRIPLDETV
                     LAELRRGSELEPRATQPARAHSLCSSAALVVNVFAYWRGRDQSPLLEALGVGGPGGTR
                     LEFEAPLPTGLPGDPPTVDVALYRPDGRCVAVESKFAEWLTPRPRGKRVFKEKYFAQG
                     QRVWDAAGLPRCQALAEDLQAGRERPRYLNAPQILKHALGLAVNGLRTSTLVYLYYDR
                     PGKEAAAHRAEIDRVVARLAGELDLRAATYQSLFAALRALQGLDGSYVDYLARRYFA"
     gene            complement(3519..4385)
                     /locus_tag="VFL84_09005"
     CDS             complement(3519..4385)
                     /locus_tag="VFL84_09005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7608800.1"
                     /translation="MVAATWRAWAAGAALAALSSTAAAELKNLAAPVIVGHYHLNVTS
                     IADHKRFWVDTLGGKAVKIGNVDAIEFPDVYLLLHEQKPTGPTRGTTFDHIGFAVPDV
                     PAMTTKVVAAGYGLTVGREPAPGETASPPTAGNYGRFSYLVGPDGVKVELVTNMAPDA
                     PPIVHHHVHFVNKQFVEMGQWYMKAFNATQRDGQTDFFFGADLPGVGYMLNFFSWLPN
                     ETLVGTKGRAVDHVGFEVRNLKAFCAELESKGLKLETPLHHDAALGLDVATLVDPWGT
                     VVELTEGLRPKP"
     gene            complement(4482..>4721)
                     /locus_tag="VFL84_09010"
     CDS             complement(4482..>4721)
                     /locus_tag="VFL84_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608801.1"
                     /translation="GFNDQGWLDGMGHPHTDRMRVTERFRRRDAGHLEIQTTIDDPGA
                     YASPFTYTIKTTLVPEDDLLEYFCSENEKDSEHYQ"
BASE COUNT          703 a         1645 c         1641 g          732 t
ORIGIN      
        1 aaggccgcgg gcctcgccgg gacgccgctc gtcgggcgcg tggtgcccga gctcgaggat
       61 cccgtagcgc cttcgattac gcgtcatacc ggccctccct cttgaatccg agtccccact
      121 gcgccgcgag caatctcaca agccgaaaag tcgttggttc ccgagtacgc ggaggtatcg
      181 caggcaagac cgacggtcgc gtctgtcaag cagttgacac aactcccaca tttgtttgct
      241 tgacgcacgc agcttcattg gcgctcaaga aacgatgacc tgacgccgtg cgatcagctg
      301 gaaccgatgc gaggcggcga cttcctagaa tgcggagtac gagcttcgcc ttacgcagcg
      361 ctagcgcagc gacagcaccg gccgcggcgc cttggccgcg ccctgcgaca cgcggatctc
      421 gaagcagcga tgaatgcgcg ggttgcgcgc gaagtccttc gggatcgtcg ccggcgtcac
      481 gtcggcgacg gcgaggccgg cgagcccggc ctcgtcgagc cggaacttgc ggaagttcgt
      541 cgagaagatc agcagcccgc tcggcgcgag cttcagcagc gtcgagcgga tcagctcgac
      601 gtggtcgcgc tgcacgtcga gctccttcgc catgcgcttc gagcgcgaca gcgtcggcgg
      661 gtcgaggaag atcaagtcgt agcgctcgcg ctcgcgttgc tcgagccatt cgagcacgtc
      721 ggcctggatc aagcggtgcc ggttcgcgtc gtcgaagccg ttgcgctcga ggttgcgctg
      781 cgcccagtcg aggtacgtgc gcgagagatc gacgctcgtc gtcgcgcgcg cgccgcccgc
      841 ggccgcgaac acggtcgccg tcgccgtgta cgcgaacagg ttcagaaatg ttttcccggc
      901 cgcgagctcg cgaatccgcg cgcgcgtcgg gcggtggtcg aggaacaagc ccgtgtcgag
      961 gtagtcggtg aagttgacga ggaacttgag cccgccctcc tcgacgacct cgaactcgcc
     1021 gcgctcggcg agcttctcgt actgcgcgcc gcgcttctgc ttgcggcgcg tgcgcacgta
     1081 cacgcgctcg cgcggcactt cgagcacctc gggcaacacg ctgaacgctt cggcacgccg
     1141 cgcgcgcgcc ttctcggcgt cgacggtttt cggcggcgcg tactcttgca cgtaggcgaa
     1201 gcgccgcgcc gaaggctcgg cggacgcggc gtagacgtcg atcgagaacg cgtactcggg
     1261 catgtccgcg tcgtagacgc gatagcaggc aacgccttcc tcgcgcgccc attcgccgag
     1321 cgaggcgacg ttcttgcgca accggttcgc gaacatctcg gcgccggggc gcgcgcgggc
     1381 ggccgcgtcg gcggccggca gcgcgcccgg cacgcgcttc tcctcgaagt gcttcggctc
     1441 gatgtcgaag cgcagcagcc ggcactcgat cgggccgttg tacatcgtgt gcgtgcgctt
     1501 cgcccggatg cgcagctcgc ggccgagcgg cgggttgccc gtcagcacgg cggcttgcca
     1561 gccgacgtag ccggccttga gcttctcacc gagcagcgcg taggttttct tcagcgcgtc
     1621 ctcgtcgccg agccgctcgc cgtacggcgg attgacggcg acgagcccgc gcggcgccgg
     1681 agccgcggga agatcctcga gctcgcgccg ctcggcgtgc acgtgcttcg cgaagcccgc
     1741 gcgcgtcgcg ttctcgagcg tcgcgcgcac ggcggccgca tcgcggtcgt aggcgcgaaa
     1801 tacgaaccgg tcgagccggg ccgcggcacg ccgctcggcg gcttccgtga gcaggcgctg
     1861 ccaggccgcc tcgtcgtggc cgcgccagcg caagaaaccg aactcgttgc gcagcaggcc
     1921 cggcgcgacg tcgcccgcga tcagcgcggc ttctattgcg aggctgcccg agccgcacat
     1981 cgggtcgacg aagccgagct cgccgccgtc ggccgcgctc gcgagcttct gccaaccggc
     2041 ccgcagcagc accgccgcgc cgaggttctc cttgagcggc gcggcgccct gcgggccgcg
     2101 atagccgcgc cggtgcaagc tctcgccgga gagatcgatg gcgagcgtcg cgacgtcgcg
     2161 gtcgagatgc agattgatgc gcacgtcggg cgcagcgacg tctacggacg gccgcgcgcc
     2221 gaagcgctcg cggaactgat cgacgatcgc gtccttcgtc ttcagcgcgg cgaactgcgt
     2281 gtgcgcgacg ctcgagcgcg tgctcgtcgc ctcgacggcc agcgtgccgt ccacgctcaa
     2341 gtgctcgctc caatcgacgg tgcgaacgcc gtcgtagagc gcctcgggcg tcggcgcgga
     2401 aaatttcgcg accgcgagca gcacgcggtt cgcgacacgc gaccagaggc acgcgcggta
     2461 cgcctgctcg agcgagcccg agcatgcgac gccgccgcgc tcgactttga ggtcggcgag
     2521 gccgcacgcg gcgagctcgt cggccgcgag atccgcggtg ccggccggcg cggtgacgta
     2581 gagggaatgc aagggcgcga cccgacttcc aagtgggccg ccatgctaag cgatggcttc
     2641 ggcgaacgca cgccgcaggc gcgctacgcg aagtagcgcc gcgcgaggta atcgacgtag
     2701 ctgccgtcga ggccctgcag cgcacgcagc gccgcgaaca gcgactggta cgtcgcggcg
     2761 cgcaggtcga gctcgcccgc gagtcgcgcc acgacgcggt cgatctcggc tcgatgcgcc
     2821 gccgcttcct tgcccggccg gtcgtagtag agatacacga gcgtgctcgt gcgcaggccg
     2881 ttcacggcga gaccgagcgc gtgcttcaaa atctgcggcg cgttcaagta gcgcggccgc
     2941 tcgcggccgg cctgcaagtc ctcggcgagc gcttggcagc gcggcagacc ggccgcgtcc
     3001 cacacacgct gcccttgcgc gaagtatttc tccttgaaca cgcgcttgcc gcgcggccgc
     3061 ggcgtgagcc actcggcgaa cttgctctcg acggcgacgc agcggccgtc gggccggtac
     3121 agcgccacgt cgacggtcgg cggatcgccg ggcaaacccg tcggcagcgg cgcctcgaac
     3181 tcgaggcgcg tgccgcccgg cccgcccacg ccgagcgcct cgagcagcgg cgactggtcg
     3241 cgtccgcgcc aatacgcgaa cacgttgacg acgagcgcgg ccgacgagca cagcgagtgc
     3301 gcgcgtgcgg gctgcgttgc ccggggctcg agctccgagc cgcgtcgcag ctcggcgagc
     3361 acggtttcgt cgagcggaat gcgcagatta tcggcgagcg cgcgcacgaa gccgcgcgcg
     3421 tcgtagcgca cgccgtgtgc gtcggcccag cgccgctgcg cgcgcaatag ctcggtctta
     3481 ccggtaacca ccggcgagag tgtaacggcg cgcgcgcctc agggcttcgg tcgcagccct
     3541 tcggtcagct cgacgaccgt gccccacggg tcgacgagcg tcgcgacgtc gagcccgagc
     3601 gccgcgtcgt gatgcagcgg cgtctcgagc ttgaggccct tcgactcgag ctccgcgcag
     3661 aacgccttca ggttgcggac ctcgaagccg acgtgatcga cggctcggcc cttcgtgccg
     3721 acgagagtct cgttcggcag ccagctgaag aagttcagca tgtagccgac gccgggcaag
     3781 tcggcgccga aaaagaaatc ggtctggccg tcgcgctgcg tcgcgttgaa cgccttcatg
     3841 taccactgcc ccatctcgac gaactgcttg ttcacgaagt gcacgtggtg atgcacgatc
     3901 ggcggcgcgt cgggcgccat gttcgtcacg agctcgacct tgacgccgtc gggcccgacc
     3961 aggtacgaga accggccgta gttgccggcc gtcggcgggc tcgccgtctc gcccggcgcg
     4021 ggctcgcgcc cgaccgtgag cccgtagccc gccgcgacga ccttcgtcgt catcgcgggc
     4081 acgtcgggca ccgcgaagcc gatgtggtcg aacgtcgtgc cgcgcgtcgg gccggtcggc
     4141 ttctgctcgt gcagcagcag gtacacgtcc ggaaactcga tcgcgtcgac gttgccgatc
     4201 ttgacggctt tgccgccgag cgtgtcgacc cagaagcgct tgtggtcggc gatgctcgtc
     4261 acgttcaagt gatagtggcc gacgatgacg ggcgccgcga gattcttcag ctccgccgcc
     4321 gccgtgctcg agagcgccgc gagcgccgcg ccagcggccc atgcgcgcca tgttgctgcg
     4381 accattgcac ctccccttac gatgaggccg atcttatacg agcgctcgat cttttgggga
     4441 tcgaatgatt cgcgcggcgc gcagcgactg gcgagcgcgg atcactggta gtgctcggag
     4501 tccttctcgt tctcggagca gaagtactcg agcaagtcgt cctcgggcac gagcgtcgtc
     4561 ttgatcgtgt acgtgaacgg gctcgcgtag gcgcccgggt cgtcgatcgt cgtctgaatc
     4621 tcgaggtgcc ccgcgtcgcg ccgacggaag cgctccgtga cgcgcatgcg atccgtatgc
     4681 ggatgaccca tgccgtccag ccacccttga tcgttgaaac c
//
LOCUS       DASSNN010000486         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_84775, whole genome shotgun sequence.
ACCESSION   DASSNN010000486 DASSNN010000000
VERSION     DASSNN010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84775"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFL84_09015"
     CDS             <1..471
                     /locus_tag="VFL84_09015"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7608802.1"
                     /translation="YTIRKVLPTAAAAALLFMTAAQAHHSFAMFDREQEIVKTGTVVR
                     WAFNNPHSWLYLNVKNADGTETLWSFEGSAPPALIQRGITGATFEPGDTVQFMYCPLR
                     DGRPGGAIGWAKLEDGQYINPADGGCAGNEQSIERWKGWLEKGYTSKADAEKAK"
     gene            769..1986
                     /locus_tag="VFL84_09020"
     CDS             769..1986
                     /locus_tag="VFL84_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608803.1"
                     /translation="MRYVPSTTVLSMAVYGGSGLAFVGANLLLARELSTTQFALLTLL
                     VALNTLGYHLAPLGLDGIVMRGRAQIGGALLKRAAAVALGVGAAMSIAASLVYGLSAA
                     MALLLLAGTAAGGLMLVASARFQSEQRFAISLALVMSPNLVLLLGAVATMLAGSSTAG
                     LSFEILTVGLGLAAGVGWALALRERRGQSAAPASVPWSEALVLAGVSAAGMLFVQLER
                     LVIPQVLSVADLALFGVLGAIAGSIFRLLQMGVGFSLLPRLRAATTILERRQLIAREL
                     RFAVVIAALGSAAILALTPPIEHWLLDGKYHLSMALLVAALFSGVAKIAHAFARATAT
                     ALATARELKLVSFAGWLSAAFAIGSAVVAAPWGLTGVIYGIGVGWLAWAVISFVVVSH
                     HLRLPAAVAAETQ"
     gene            complement(2002..2472)
                     /locus_tag="VFL84_09025"
     CDS             complement(2002..2472)
                     /locus_tag="VFL84_09025"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET7608804.1"
                     /translation="MEDAAHLYDLLKGFDTAMLVTRSVDGHLHARPMLVAELHADADA
                     YFVTSADSPKVAEVYADADVLLTFQSATQYAAVYGRVNVVRDRALVERLWSDAWKVWF
                     PRGAVDPSIALLRFDAEHGEFWNDLSARGVRYAFETAKAYMIGNSRRGERSPKH"
     gene            complement(2485..2865)
                     /locus_tag="VFL84_09030"
     CDS             complement(2485..2865)
                     /locus_tag="VFL84_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7608805.1"
                     /translation="MIAHTSLAVRDFKAARMFYERALAPLGYSPKMQHEESTGFNDGK
                     NTDFWIGKRKNVEPTHVAFEAKGKDEVEAFYSAALAAGGRDNGGPGYRRDYWPGYYAA
                     FVYDLDGHNIEAVWYDYAAEGAKD"
BASE COUNT          466 a         1062 c         1042 g          589 t
ORIGIN      
        1 tacacgattc gcaaagtgct gcccacggct gcggcggccg cgctgctttt catgacggcg
       61 gcgcaagctc atcactcgtt cgcgatgttc gaccgcgagc aggagatcgt gaaaaccggc
      121 acggtcgtgc gctgggcctt caacaatccg cactcgtggc tctacttgaa cgtcaagaac
      181 gcggacggca ccgagacgct ctggagcttc gaaggctcgg cgccgcctgc gctgatccag
      241 cgcggcatca cgggcgcgac gttcgagccc ggcgataccg tgcagttcat gtactgcccc
      301 ttgcgcgacg gccggcccgg cggcgcgatc ggctgggcaa agctcgagga cggccagtac
      361 atcaaccccg ccgacggcgg ctgcgccggc aacgagcaga gcatcgaacg gtggaagggc
      421 tggctcgaga agggctacac ctcgaaggcc gacgccgaaa aggcaaagta acggctgcgt
      481 cacccgctaa aaggggacag atctattttc agggaccgcg aaaggggacg gatctatttt
      541 tcttctgaaa atagatccgt ccccttttag cgttcagaaa atagatctgt ccccttttga
      601 cgcggccggt tcacggcttt tggcgtcgtt tccagaaatt tcatagccat tgaggacgcg
      661 cgcgcggcga gcggcagcga agccgctggc acgatgattg ctcgtcgtct cccgtacgcg
      721 tcggcgtctg ttgcacggcg caagcccgct gacgaggaga gacgactgat gcgctacgtc
      781 ccatctacga ccgtgctcag catggccgta tacgggggtt ccgggctcgc gttcgtgggc
      841 gcgaacttgc tgctcgcgcg cgagctgtcg acgactcaat tcgcgttgct gacgctgctg
      901 gtggcgctca acacgctcgg ctatcacctg gcgccgctcg gcctcgacgg catcgtcatg
      961 cgcggccgcg cccaaatcgg cggcgcgctg ctcaaacgcg ccgccgccgt tgcgctcggc
     1021 gtcggcgcgg cgatgagcat tgccgcgtcg ctcgtttacg ggctttcggc cgcaatggcc
     1081 ttgttgctgc tcgccggcac ggcggcgggc ggcctcatgc tcgtcgcgag cgcgcggttc
     1141 cagagcgagc agcggttcgc aatctcgctc gcgctcgtga tgtcgccgaa cctcgtgttg
     1201 ttgctcggcg ccgtcgcgac gatgctcgcg gggtcgagca cggccggttt gtcgttcgag
     1261 atcctgacgg tgggtctcgg cttggccgca ggcgtcggct gggcgctcgc gctgcgcgag
     1321 cgccgaggcc aaagcgccgc gccggcgtcg gtgccgtgga gcgaagcgct cgtcctcgcc
     1381 ggcgtcagcg ccgccggcat gctgttcgtg cagctcgagc ggctcgtcat cccgcaggtg
     1441 ctttcggtag cggacctcgc gctgttcggc gtgctcggcg cgatcgcggg atccatcttc
     1501 cggctgctgc agatgggcgt cggcttcagc ctgctgccgc ggctgcgcgc cgccacgacg
     1561 attctcgagc gccgccagct catcgcccgt gagctgcgct tcgccgtcgt catcgcggcg
     1621 ctgggctcgg ctgcgatact cgcgttgaca ccgccgatcg agcattggct gctcgacgga
     1681 aagtatcacc tctcgatggc gctactggtc gcggcgttgt tcagcggcgt cgcgaagatt
     1741 gcacacgcgt ttgcgagggc cacggccacc gcgctcgcga cggcgcgcga gctcaagctc
     1801 gtgagcttcg caggttggct gtcggcggcg ttcgcgatcg gctctgccgt cgtcgcggca
     1861 ccgtggggcc tcaccggcgt catctatggg atcggcgtcg gctggctcgc gtgggcggtc
     1921 atttcgttcg tcgtcgtcag ccaccacttg cggttgcccg cggccgtcgc cgcggagacg
     1981 cagtaggccg ccgatctcga gctagtgttt tgggcttcgc tcgccgcgcc gcgagttgcc
     2041 gatcatgtag gccttcgcgg tctcgaacgc gtagcgcact cctcgcgcgc tcaggtcgtt
     2101 ccagaattcg ccgtgctcgg cgtcgaaccg cagcagcgct atcgacggat ccaccgcccc
     2161 gcgcgggaac cagaccttcc aggcatcgct ccacaaccgc tcgacgagcg cgcgatcgcg
     2221 aacgacgttc acgcggccgt acacggctgc gtactgcgtc gcgctctgaa acgtcaacag
     2281 cacgtcggcg tcggcgtaga cctcggcgac cttcggcgaa tccgcgctcg tgacgaagta
     2341 tgcgtccgcg tcggcatgca gctcggcgac gagcatcggg cgcgcgtgca agtgcccgtc
     2401 gacggacctc gtcacgagca tcgccgtgtc gaatcctttc aagagatcgt agaggtgcgc
     2461 ggcgtcttcc attgcgcagt gccttcagtc cttcgcgccc tcggcagcgt agtcgtacca
     2521 aacggcttcg atgttgtggc cgtccaagtc gtagacgaat gcggcgtagt agcccggcca
     2581 gtagtcgcgc cggtagccgg ggccgccgtt gtcgcggccg ccggccgcga gcgcggcgct
     2641 gtagaacgcc tcgacctcgt ccttgccttt ggcctcgaac gcgacgtgcg tcggctcgac
     2701 gttcttgcgc ttgccgatcc agaagtccgt gttcttgccg tcgttgaagc ccgtcgactc
     2761 ctcgtgctgc atcttcggcg agtagccgag cggcgcgagc gcccgctcgt agaacatcct
     2821 cgccgccttg aagtcgcgaa ccgccaaact cgtatgcgcg atcatcgagc ctccatgggc
     2881 cgcgtacccg tcggaaaaaa agaggcaagt catgtgccag cggccgttcg ccgccggttt
     2941 cttgtaagct cctgaacaca tcccactggc cgcggtcccg aaccgcgcgg accaatcgat
     3001 cgatgccgag gctcgcttac aacgagaacg gatcacgcga ggctttagtg tgctcctcgc
     3061 gctgatcgtc cttctgccct tcgtcggcag cgtctgtgcc gccttcatgc cggccaacgc
     3121 gcgcaacgcc gaggcgtggc tcgcgggcgc cgtggcggc
//
LOCUS       DASSNN010000487         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_825654, whole genome shotgun sequence.
ACCESSION   DASSNN010000487 DASSNN010000000
VERSION     DASSNN010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825654"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VFL84_09035"
     CDS             <1..987
                     /locus_tag="VFL84_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608806.1"
                     /translation="GLNDAHGKKATIRNGAQALTKQTAQPTLFSIENLRFEQPLFSAI
                     SILGAGPASSTKISDVNIVGVRTFLFPAFNARFREGISVSTALAPIGGDIEISNNVID
                     GGTYTADDTTLAVSSAIAVTGALPQPGPANQPFTARLRISDNKLVNWSGSGILAAGVD
                     ELTIERNVIQPGAFANTLPLGCTTANGLGSANGISLQSVRDATVRDNAITLVPAVTGA
                     GTPATCTAGLILVGPTNVAGGVADGNIVYRNRIKGSGTYAIVVGVTATLPPGPTVSVE
                     SDNLFALNRLGNFAPLGASLFLGPGANGNAFVGNFPSLAGNVAGNDVISRQP"
     gene            complement(1108..1845)
                     /locus_tag="VFL84_09040"
     CDS             complement(1108..1845)
                     /locus_tag="VFL84_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608807.1"
                     /translation="METKKIRFVANKPWLTLDSPSKPGPIVKTLPDWYSKAEWFHQDP
                     STGKPFVEPDGGRVPTWKACPAVLDVMGTGYTYKTPCDIEFAEDSAGNIHGRVLDEQY
                     RSFLQEREPMPQFFHPRGFHRKHFAWWADWAVELPEGYSALYTHPLNRFELPFWTTSG
                     IVDNDKVHLPGTMPFFMLKGVRGILPAGTPYAQIIPFKREHWESEIDVSLTAEEISAK
                     TKENVTKYRKPDGGVYQREVWERRKYQ"
     gene            2072..>2631
                     /locus_tag="VFL84_09045"
     CDS             2072..>2631
                     /locus_tag="VFL84_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7608808.1"
                     /translation="MSVIDFLGSIGRDLRHAARGLARRPAFTMAAVLTLAIGIGATTA
                     IFSVVYSVLIKPLPYPNAGELVRIRYSSATLGTEELSSSTTQYFTYRAESRTIAEIGL
                     WGNGGETVTTADGTERIRSLRVTYGVLQALAVQPARGRWFTEQEHGPAAEGPDPLIVS
                     YGFAQSRYGSDSAAIGRDLTVNGRPA"
BASE COUNT          427 a          887 c          856 g          461 t
ORIGIN      
        1 ggattgaacg acgcccacgg aaaaaaggcg acgatccgca atggcgcgca ggcactgacg
       61 aagcagacgg cgcagccgac gctcttcagc atcgagaacc ttcggttcga gcagccgctg
      121 ttctcggcga tctcgattct cggcgctggc ccggcaagct ctacgaagat ctccgacgtg
      181 aacatcgtcg gcgtgcggac gtttttgttc ccggctttca acgcgcggtt ccgcgaaggt
      241 atctcggtgt cgacggcctt ggcgccgatc ggcggcgaca tcgagatttc gaacaacgtc
      301 atcgacggcg gcacgtacac ggccgacgac actacgcttg cggtcagctc cgccatcgcg
      361 gtcaccggcg cactgccgca gcccggtccg gccaaccagc cgttcaccgc gcgcctgcgc
      421 atttccgaca acaagctcgt gaactggtcg ggcagcggca ttctggcggc cggcgtcgac
      481 gagctcacga tcgagcgcaa cgtgatccag cccggcgcgt tcgcgaacac gctgccgctc
      541 ggctgcacga cggcgaatgg cctcggctcc gcgaacggca tcagcctcca gagcgtccgc
      601 gacgccaccg tgcgcgacaa cgcgatcaca ctcgtgccgg ccgtcaccgg cgccggcact
      661 ccggccacgt gcaccgcggg cctgatcttg gtgggaccga cgaacgtcgc aggcggcgtc
      721 gccgacggca acatcgttta tcgaaaccgc atcaagggca gcggcacgta tgcgatcgtc
      781 gtcggcgtga cggctacgtt gccgccgggc cccaccgtct cggtcgagag cgacaacttg
      841 ttcgcgctga accggctcgg caacttcgcg ccgctcggcg cttcgttgtt cctcggcccg
      901 ggcgcgaatg gcaacgcgtt cgtgggcaat ttcccgtctc tcgcgggcaa cgtggccggc
      961 aacgatgtga tcagccgcca gccttgaggc gagaaaacgc gcgccaggcg gtgcagcgga
     1021 tcgctcaggc cgcgcgccgc ccgctgcgcc gtttcggcgc gtagggcggc gcgggcaagt
     1081 gcaggccgct gaacatcagc aacgcgtcta ctggtatttg cggcgctccc agacctcgcg
     1141 ttggtaaacg ccgccatcgg gcttgcgata tttcgtgacg ttctctttgg tcttggcgct
     1201 gatctcttcc gcggtcaacg acacgtcgat ctccgattcc cagtgctcgc gcttgaacgg
     1261 gatgatctgc gcatacggcg tgccggccgg gaggattccg cggacgccct tcagcatgaa
     1321 gaacggcatc gtgcccggca gatgcacctt gtcgttgtcg acgatgccgc tcgtcgtcca
     1381 gaacggcagc tcgaatcgat tgagcgggtg cgtatagaga gcgctgtagc cttccggcag
     1441 ctcgacggcc caatcggccc accacgcgaa gtgcttcctg tgaaagccgc gcgggtgaaa
     1501 gaactgcggc atcggctcgc gctcttgcag aaagcttcga tactgctcgt cgagcactct
     1561 gccgtggatg ttgccggctg agtcctcggc gaactcgatg tcgcacggag tcttgtaggt
     1621 gtagcccgtg cccatgacgt cgaggaccgc ggggcacgcc ttccatgtcg gcacgcggcc
     1681 accgtccggc tccacgaacg gtttgccggt cgacgggtcc tgatggaacc actcggcctt
     1741 gctgtaccaa tcgggcagcg tcttgacgat cggcccgggt ttcgacgggc tgtccagcgt
     1801 cagccaaggc ttgttcgcca cgaaccggat ctttttggtt tccaccgctc gattcgccct
     1861 cttcttgtcg cacgctgtgg cgccgccgag tatgttgcag ccgtcccgcc ggcgggaagc
     1921 ccctgtgcgg atccgggacg cggccggttc gcgagtcgcg cggcgagccc gcaggcccgc
     1981 gcgaatcaac gtcttgcgcc agccgcggtt ttggcacgaa tgtcgcaaac gcgcagctta
     2041 gcgagctaca cggcggggga tctcgagcct gatgagcgtc atcgacttcc ttggatccat
     2101 tggccgcgat ctgcgccacg cggcgcgcgg gttagcccgc cgaccggcat tcacgatggc
     2161 ggccgtgctc acactcgcga tcggcattgg cgcgacgacg gcgatcttca gcgtcgtcta
     2221 ctcggtgttg atcaagccgc tgccgtatcc gaacgccggc gagctcgtgc gaatccgcta
     2281 tagctcggct acgttgggca ccgaagagct gtcgtcgtcg acgacgcagt acttcaccta
     2341 tcgcgccgag agccggacga tcgccgagat cgggctctgg ggcaacggcg gcgagaccgt
     2401 gacgaccgcc gacgggacgg agcgcatccg ttcgctgcgt gtgacatacg gtgtgctaca
     2461 ggcgctcgcc gtgcagcccg cgcgcggacg ctggttcacg gagcaggagc atgggcccgc
     2521 ggccgagggc cccgatccgc tcatcgtttc ctacggattc gcgcagagcc ggtacggcag
     2581 cgattcggcc gcaatcggac gcgacctcac cgtcaacggc cggcccgcgc a
//
LOCUS       DASSNN010000488         3249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_948034, whole genome shotgun sequence.
ACCESSION   DASSNN010000488 DASSNN010000000
VERSION     DASSNN010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948034"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFL84_09050"
     CDS             <1..378
                     /locus_tag="VFL84_09050"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7608809.1"
                     /translation="VELAIELGNDLNAVADFGDFEMVGEPEYTLLYYPHNLRELQDLG
                     VGDPRWSGSTALHGSIISNQPSIVQYLVDHGANIDAKTETGWTALMMTRGVFLANTGR
                     LFPAAEAILEKAHARRSAQAAAN"
     gene            498..1991
                     /locus_tag="VFL84_09055"
     CDS             498..1991
                     /locus_tag="VFL84_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7608810.1"
                     /translation="MAAEITEPTADSAVAPARVRATRRRLGIALFALAGVAAAGGYYW
                     YRASHAASGDRPLVVAVSIGDVENAVASAGTLQPSNTVPVGAQVSGQLQKLYVDVGDQ
                     VEMGQLLGEIDARVQRNKVDSSRASIASAEAQITARREALVLAQANWERQQRLWEDQA
                     TSKQEYDSARNNLASAQASLTQQEQSIIQSKATLATDETLLEYTKIFAPIGGTVVSIA
                     MKEGTTLNATQQSPTIMNIANLATMTVETQISEADIGKLYPGMQVYFTTLGGGNRRWH
                     STLRQILPTPTATNNVVTYTGLFDVDNSDGALLSGMTTQVYFVISAARNVLTVPVGAL
                     TFVDSPRSDGFAGRAAERSAAPGASGWRGQAADAGGSPAPSERAVLVSSSPSGTYPGG
                     AFDAARRSGSGSTPGRAQPSRAAKVRVVAADGSTAERDVMIGVTSRVTAEVLSGLTEG
                     EQVVAGIAQAAQRPGVGGQSNNGNNNFNNNFNRGFPGGNNFPGGFPR"
     gene            2001..>3249
                     /locus_tag="VFL84_09060"
     CDS             2001..>3249
                     /locus_tag="VFL84_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7608811.1"
                     /translation="MIAHALDATAAPDVIEDAGPDLTSRTVAVPLIDLRGVSRTFVTD
                     GGVEVRALREIDLTIYPGEFIAIMGQSGSGKSTLMNILGCLDRPSSGRYLFAGRDIQS
                     FDADGLAWLRREAFGFVFQSYNLLPSETAKENVEVPGIYAGLSASERAVRSESLLTTL
                     GLGERLDHRPNQLSGGQQQRVSIARALMNGGKIILADEPTGALDSRSGVEVMALLETL
                     ARRGHTVILITHDAEVARLADRIVEFSDGRVVRDSGRKPDAVAAKRNAELASLFMSRR
                     ASSLLGGSGEAIRMAMSSLRANLFRTILTLLGIVIGVASVVAMLAIGEGVQSGVIERI
                     SAIGTNMLVLQPARAENQRRNLPSTLAFSDADAILDNVPNVLYAMPELQNSQTVRWGN
                     ADYSTRITAASDVFPRARNWPMAR"
BASE COUNT          562 a         1087 c         1104 g          496 t
ORIGIN      
        1 gtcgagcttg cgatcgagct cggcaacgac ttgaacgccg tcgcggactt cggcgacttc
       61 gagatggtcg gcgagcccga gtacacgctg ctctactatc cgcacaatct gcgcgagctt
      121 caggatctcg gcgtcgggga cccgcgctgg agcggcagca cggccctgca cggctcgatc
      181 atctcgaacc agccgtcgat cgtgcagtac ctcgtcgatc acggcgccaa catcgacgcg
      241 aagaccgaga ccggctggac ggcgctgatg atgacgcgcg gcgtgttcct cgcgaacacg
      301 ggccggctgt tcccggcggc cgaggcgatc ctcgagaagg cgcacgcgcg gcgcagcgcg
      361 caggcggccg cgaactaagc gattccggcg gctccccggg ccgagccggg gcggcctttg
      421 tgcttctaaa gggacacccc cgcgaaactc ggctacgttg cgctgcaatt tcacggtatt
      481 cgcggagtgg gaggaacgtg gccgccgaaa tcactgagcc gacagcggat tccgccgttg
      541 cgccggcgcg cgtgcgagcc acgcgccgcc gcctaggaat cgcgcttttt gcgctcgccg
      601 gcgtagcagc ggcgggcggc tactactggt accgtgcttc gcacgcagcg tccggggacc
      661 ggccgctcgt cgtggcggta tcgatcgggg acgtcgagaa cgcggttgcg tcggcgggca
      721 ccttgcagcc gagcaacacc gttcccgtcg gcgcgcaggt ctcgggccag ctccaaaagc
      781 tctacgtcga cgtcggcgat caagtcgaga tgggacagct gctcggcgag atcgatgcgc
      841 gcgtgcagcg caacaaggtc gactcgagcc gcgcgagcat cgcttcggcc gaggcgcaga
      901 tcacggctcg gagagaggcg ctcgtgctcg cccaagcgaa ttgggagcgc cagcagcgtc
      961 tctgggaaga ccaggcgacg agcaagcagg aatacgactc ggcgcggaac aacctcgctt
     1021 cggcgcaggc gagcctcacg cagcaagagc agtcgatcat tcagagcaag gcgacgctcg
     1081 cgaccgacga gacgctgctc gagtacacga aaatcttcgc ccccattggc ggcaccgtcg
     1141 tgtcgattgc gatgaaagaa ggcacgacgc tgaacgcgac gcagcagtcg ccgacgatca
     1201 tgaacatcgc gaacctcgcg acgatgacgg tcgagacgca gatctcggaa gccgacatcg
     1261 gcaagctcta tcccggcatg caggtttact tcacgacgct cggcggcggc aatcgccgct
     1321 ggcacagcac gctacggcaa atccttccta cgccgacggc gaccaacaac gtcgtgacct
     1381 acaccgggct cttcgacgtc gacaatagcg acggcgcgtt gttgtccgga atgacgacgc
     1441 aggtctattt cgtgatatcg gctgcgcgga acgtcctcac ggtgcccgtt ggcgcgctga
     1501 cgttcgtcga ctcgccgcgc agcgacggct ttgcgggacg cgccgccgag agaagtgccg
     1561 cgcccggcgc ctcgggctgg cgcgggcagg ccgctgacgc gggcggctct ccggcgccct
     1621 ccgagcgcgc agtcctggtc agtagctcgc cgagcgggac gtaccccggc ggcgcgttcg
     1681 acgccgcgcg cagaagcggc agcggttcca cgcccggccg ggcgcaaccg tcgcgcgcgg
     1741 ccaaggtgcg cgtcgttgcc gccgacgggt cgaccgcgga gcgcgacgtc atgatcggcg
     1801 taacgagccg cgtgacggcc gaagtgctct cgggcttgac cgaaggcgag caagtcgtcg
     1861 cgggcatcgc gcaagccgcg caacgccccg gcgtcggcgg ccagagcaac aacggcaaca
     1921 acaacttcaa caacaacttc aatcgcggct ttccgggcgg caacaacttt cccggcggct
     1981 ttccgcgata ggcgcgagcc gtgattgcac acgccctcga tgcaacggcg gcgcccgacg
     2041 tcattgagga tgccgggccg gatctgacga gccgcacggt cgccgtcccg ctcatcgatc
     2101 tgcgcggggt ttcgcgtacg ttcgtcactg acggcggcgt cgaagtccgc gcgctgcgcg
     2161 aaatcgacct gacgatctat cccggcgagt tcatcgcgat catgggccag tcgggctccg
     2221 gcaagtccac gttgatgaac atcttgggct gcctcgatcg gccgagcagc ggccggtatc
     2281 tcttcgcggg ccgcgacatc cagagcttcg acgcggacgg cctcgcatgg ctgcgccgcg
     2341 aagcgttcgg ttttgtgttc cagagctaca acctgttgcc gagcgagacg gcgaaggaga
     2401 acgtcgaagt gcccggcatc tacgcgggcc tctcggcatc tgaacgcgcg gtgcgctccg
     2461 aatcgctgct cacgacgctc ggcttgggcg agcgtctcga tcaccggccg aaccagctgt
     2521 cgggcgggca gcagcagcgc gtctcgatcg cgcgcgcgct catgaacggc ggcaagatca
     2581 ttctcgccga cgagccgacc ggcgcgctcg attcgcgcag cggcgtcgag gtcatggcgc
     2641 tgctcgaaac gctggcgcgc aggggccaca cggtgatcct gatcacgcac gacgccgaag
     2701 tcgcgcggct tgccgaccgc atcgtcgagt tcagcgacgg ccgcgtcgtg cgcgacagcg
     2761 gccgaaaacc ggacgcggtc gccgccaagc gcaacgccga gctcgcgagt ctcttcatga
     2821 gccggcgcgc gtcgtcgctg ctcggcgggt ccggcgaggc gatccgcatg gccatgagct
     2881 cgttgcgagc gaatctgttc cgcacgatcc tgaccttgct cggcatagtg atcggcgtcg
     2941 cgtccgtcgt cgcgatgctc gcgatcggcg agggcgtgca gtcgggggtc atcgagcgca
     3001 tcagtgcgat cggcacgaac atgctcgtgc tgcagccggc gcgtgccgag aatcagcggc
     3061 gcaacctgcc gtccacgctc gcgttctcgg acgccgatgc gatcttggac aacgtgccaa
     3121 acgtgctcta tgccatgccc gagttgcaga acagccagac ggtgcgctgg ggcaatgcgg
     3181 attactcgac gagaatcacg gcggcttccg acgtgttccc gcgagcgcga aactggccga
     3241 tggcgcgcg
//
LOCUS       DASSNN010000489         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1097303, whole genome shotgun sequence.
ACCESSION   DASSNN010000489 DASSNN010000000
VERSION     DASSNN010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097303"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VFL84_09065"
     CDS             complement(<1..1037)
                     /locus_tag="VFL84_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7608812.1"
                     /translation="MTASPLTRSLAAAALLALLATAAHSATPRLVQAVKDGDRDAALA
                     LARDANEVAATEADGTTALHWAVRQGDFELVDRLLRSGAAVDAANRYGVTPLKLAAIS
                     GDAKLLKRLLDAGGDANTVGTDGETLLMTAARGGHVDAARLLIERGAEVDARESWHGQ
                     TALMWAAAQGHPAMVRELIAHGADVNARSNQEVWERQVTSEPRDKWLPPGGLTPLLFA
                     ARENCLDCLPVLIEAGADLNATTPDGISAVVIALINGHYDVAGALLEAGTDPTLADYT
                     GRTALYAAIDFNTMVKSNRPAPKVLENRLTGLDVARIALERGADVNARLKRLPPYRAK
                     LDRGNDGMLGGG"
     gene            complement(1034..2362)
                     /locus_tag="VFL84_09070"
     CDS             complement(1034..2362)
                     /locus_tag="VFL84_09070"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET7608813.1"
                     /translation="MFISKKQLPRRTFLKGLGATVALPLLDAMVPAFAATPTKAPRLG
                     FVYVPHGMVMSRFTPDAAGKGFVFKDINRSLEPYRDRLTIVSRLHHRAADSTAVHSLS
                     PTTWLSGVRPKPTQGTDAYAGVTADQIAARVIGQDTLLPSMELATEDHSGLIGACDRD
                     YGCIYMNTLSWRTPTTPMPMEINPRKVFERMFGQGDSEAARLVRRQEDRSILDAVTAQ
                     ATGLQAKLGTHDRATLDNYLEGVREIERRLTRAESQVAANADITIPEAPAGVPFEYTD
                     HVRLMYDLLALAYRADITRVFTFMMGREVSNRTYTQVGVTEGHHAVSHHQNKPEKLEM
                     LARIQAFHIGLFGEFLAKLDAIPDGDGSLLDNSMLLYGSNMSNSNAHDHYPLPNVLVG
                     GVRGRLKGGQHIVAEEHTPMTNLLLTMLDKVDAGVESLGDSTGTIAELAA"
     gene            complement(2547..>2836)
                     /locus_tag="VFL84_09075"
     CDS             complement(2547..>2836)
                     /locus_tag="VFL84_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608814.1"
                     /translation="VRPWPRTEATHIVERVYLTKLANVAARATGFVATVEKPINDDVL
                     VDEITLTDPSLYAGPQRRTAYFQRMADTATLEYACSAEHWLDALEKNRVSQ"
BASE COUNT          424 a          935 c          983 g          494 t
ORIGIN      
        1 ccgccgccga gcatgccgtc gttgccgcga tcgagcttcg cgcgatacgg cggcagccgc
       61 ttgagccggg cgttcacgtc ggcgccgcgc tcgagcgcga tgcgcgccac gtcgaggccg
      121 gtcaaacggt tctcgagcac cttcggcgcc ggccggttcg acttgaccat cgtgttgaag
      181 tcgatcgccg catagagcgc cgtgcgcccc gtgtagtcgg cgagcgtcgg atcggtgcct
      241 gcttcgagca gcgcgcccgc cacgtcgtag tggccgttga tgagcgcgat gacgaccgcg
      301 ctgatcccgt ccggcgtcgt cgcgttcaag tcggcgccgg cttcgatcag caccggcaag
      361 cagtcgaggc agttctcgcg cgcggcgaac agcagcggcg tcaagccgcc gggcggcaac
      421 catttgtcgc gcggctcgga cgtgacttgc cgctcccaga cctcttggtt cgagcgcgcg
      481 ttgacgtcgg cgccgtgcgc gatgagctcg cgtaccatcg cgggatggcc ctgcgccgcg
      541 gcccacatca acgcggtctg gccgtgccag ctctcgcgcg catcgacctc ggcgccgcgc
      601 tcgatcagga gccgagcggc gtcgacgtgc ccgccgcgag ccgccgtcat cagcagcgtc
      661 tcgccgtcgg tgccgaccgt gttcgcgtcg ccgcccgcgt cgagaagccg cttcaggagc
      721 ttcgcgtcgc cgctgatggc cgcgagcttg agcggcgtca ccccataacg attcgcggcg
      781 tcgacggcgg cgccggagcg cagcaagcgg tcgacgagct cgaaatcgcc ttgccggacg
      841 gcccagtgca gcgcggtcgt gccgtcggct tcggttgcgg cgacctcgtt cgcatcccgc
      901 gcgagcgcca atgccgcatc gcggtcgccg tccttgacgg cctgcacgag acgcggcgtc
      961 gccgagtggg cggcggtcgc cagcagcgcg agcagcgccg cggcggccag cgagcgagtg
     1021 agcggagacg ccgtcacgcg gcgagctcgg cgatggtgcc cgtgctgtcg ccgagcgact
     1081 ccactccggc gtcgaccttg tcgagcatcg tcaggagcag gttcgtcatc ggcgtgtgct
     1141 cctcggcgac gatgtgctgg ccgcccttga ggcggccgcg gacgccgccg acgagaacgt
     1201 tcggcagcgg atagtggtcg tgcgcgttgc tgttgctcat gttgctgccg tagagcagca
     1261 tcgaattgtc gagcaacgag ccgtcgccgt cgggaatcgc gtcgagcttc gccaagaact
     1321 cgccgaacag gcctatgtgg aacgcctgaa tgcgagcgag catctcgagc ttctcgggct
     1381 tgttctggtg gtgcgatacg gcgtgatggc cctcggtgac gccgacctgc gtgtaagtcc
     1441 ggttgctgac ctcgcggccc atcatgaacg tgaacacgcg cgtgatgtcg gcgcggtagg
     1501 cgagagcgag caagtcgtac atcaaccgga cgtgatccgt gtactcgaac ggcacgccgg
     1561 ccggcgcttc aggaatcgtg atgtcggcgt tcgccgcgac ttgcgactcg gcgcgcgtca
     1621 agcgccgctc gatctcgcgt acgccctcga gatagttgtc gagcgtcgcg cggtcgtgcg
     1681 tgccgagctt cgcctgcaag cccgtcgctt gcgcggtcac ggcgtcgagg atgctgcggt
     1741 cctcctggcg gcggacgagc cgagcggctt cgctgtcgcc ttgaccgaac atgcgctcga
     1801 acaccttgcg cggattgatc tccatcggca tcggtgtcgt cggcgtgcgc cacgacagcg
     1861 tgttcatgta gatgcagccg tagtcgcgat cgcaggcgcc gatcaggccg gagtggtcct
     1921 ccgtcgcgag ctccatcgag ggcagcaacg tgtcttggcc gatcacgcgc gccgcgatct
     1981 ggtcggccgt gacgccggcg tacgcgtcgg tgccttgcgt cggcttcggc cgaacgcccg
     2041 agagccaggt cgtcggcgac agcgaatgga ctgcggtcga gtcggccgcg cggtggtgca
     2101 ggcggctcac gatggtcaat cggtcacgat agggctcgag cgagcggttg atgtccttga
     2161 agacgaagcc cttgcccgcc gcgtccggcg tgaagcggct catgaccatg ccgtgcggca
     2221 cgtagacgaa accgaggcgc ggcgccttcg tcggggtcgc ggcgaacgcg ggcaccatcg
     2281 cgtcgagtag cggcaaggca accgtagcgc cgaggccttt cagaaaagtg cggcgcggga
     2341 gctgtttctt gctgatgaac atttcgcgat ttctccgccc ctgataggcc cttaactgat
     2401 tgaaaagtgt agcgaatact gaaccagaac ccaattgcgc ccggcgctgc gcgagcgcac
     2461 gattctcaca cgaaaagttt aacgtaagcc cttgccggca gtcgcgctcg gatttccgta
     2521 agccgctgtt tcgacatgca tccgggctac tgggacactc gatttttctc aagggcatcg
     2581 agccagtgct ctgccgagca cgcgtactcg agcgtggcgg tatcggccat gcgctggaaa
     2641 tacgccgtgc gccgctgcgg ccccgcatag agcgacgggt ccgtcagcgt gatctcgtcg
     2701 acgagcacgt cgtcattgat cggcttctcg acggtcgcga cgaagccggt cgcgcgtgcc
     2761 gcgacgttgg ccagcttcgt gaggtagaca cgctcgacga tgtgcgtcgc ttcggtgcgc
     2821 ggccacggcc gcacct
//
LOCUS       DASSNN010000490         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1216101, whole genome shotgun sequence.
ACCESSION   DASSNN010000490 DASSNN010000000
VERSION     DASSNN010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216101"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..772
                     /locus_tag="VFL84_09080"
     CDS             215..772
                     /locus_tag="VFL84_09080"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7608815.1"
                     /translation="MAADEPIATDSPGATIASLQQALVAARDKPNATVDERYRALEPA
                     IVETHNLPYIAEFALRRQWAALTDRERQRFVGAFQRLSVMTYAARFGTVTRETFQPIE
                     AGAPDANGRVEVTTAIKREGQPDVTLQYLLQRDGESWRIINIVADGVSDLALKRAEYQ
                     RVFAAGGIDGLIAELEQQTQRLERG"
     gene            complement(776..1354)
                     /locus_tag="VFL84_09085"
     CDS             complement(776..1354)
                     /locus_tag="VFL84_09085"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET7608816.1"
                     /translation="MARVTEKTFAAVGTLLVLCACGIASAQEVALATNTPESTFLMDA
                     IRGDIAEIKMGELAQMKGQSEGVRDFGEMLVEDHTKAQKKTADLAKEMNVPVPTQPTP
                     EQTAKHDALARLSGAEFDRQFVAEMVKGHQEEIAKYEKQAQSGDSKVAELAEDLLPTL
                     RQHLATAQRLQSGGDASHGDASHDRTPERSNN"
     gene            complement(1462..2061)
                     /locus_tag="VFL84_09090"
     CDS             complement(1462..2061)
                     /locus_tag="VFL84_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608817.1"
                     /translation="MLKRSALLLVSASLAAASASAQTATTYKSAVVALTEDAALRQSF
                     ESELVAIAKAHDYDAVTSYDITSDVKSLDTDEFLKALSDRGVQAVLMVRPAAVGEGSS
                     LEAVRAEVPSRTFADMQKFAKRPSGPGADELIAVVHLAIYTLTAKKPELVSSGAVWLD
                     EPVADRAEGIKRLQGLILANVDAVRPSIRRHLGLAPLPQ"
     gene            complement(2073..3536)
                     /locus_tag="VFL84_09095"
     CDS             complement(2073..3536)
                     /locus_tag="VFL84_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET7608818.1"
                     /translation="MTEEVGISGFAVYVPPYRVNLESWCRWTNNVWDKTRAVVGHSFR
                     RRGPAQSVYTMAATAVMRLIDRYDIDPARVGFLGLGTESSTDNSAGAVIVKGMLDEGL
                     QASGRAPLSRHCEVPEVKHACLGGVYALKHALRYLALEPEDRCAIVVSADIAEYARGS
                     SGEPTQGAGAVAMLVERNPRLLKVHLEHIGSASSYRAVDFRKPVLRNIIRGKLNCHFQ
                     DLPVFNGKYSTTCYLDETLHALNDMARRMGREPSDYYRELAAIFMHRPYHRMPEQSFA
                     MSYLFGLAQDGTRGLAELEELCARANMPVAGLLEEMRSQPNILQLVKDGNIDADAYPL
                     SIQLLKEFRGSKEFGALVASKMSLGSEPMTDIGNVYCAALPAWMAAGLEDAHRRGADL
                     GGRKVLAVGYGSGDAAEAIPMTVAREWRDAAAKIGFEAALKAHQDLTQAQYESLHDTG
                     TAQGLRDPEQGFVIQSIGSSANPNFSDEGIEYYRYVR"
BASE COUNT          600 a         1220 c         1174 g          654 t
ORIGIN      
        1 cgaaccttct tgcgccgcgg cacgtggtcg atgccgccgt tccgcccgac cgaggtcacg
       61 gaaggcgaca tccaggacat tgccgcatac ttgaagcagt cgtcgactgc cgccgcgcgc
      121 ggcgcccgct aagcttttgg agtcagggat gacaagccgc cgcgtattca ttgtttgcgc
      181 cgcaaccgcg ccgttggctt tcgccgtacc ggccgtggcg gcagacgagc cgatagcgac
      241 cgactcgccc ggcgcgacga tcgcgtcatt gcagcaggcg ctggtcgcgg ctcgcgacaa
      301 gccgaacgcg accgtcgacg agcgctatcg cgcgctcgag ccggctatcg tcgagacgca
      361 caacctgccg tacatcgccg agttcgcgtt gcgccggcag tgggccgcgt tgacggatcg
      421 agagcggcag cgcttcgtcg gggcgttcca acgcctgagc gtcatgacct atgccgcgcg
      481 cttcggcacc gtcacgcgtg aaacgtttca gccgatcgag gcaggggcgc ccgacgcgaa
      541 tgggcgcgtc gaggtcacga cggcgatcaa gcgcgaaggt cagcccgacg tcacgcttca
      601 gtatctgctc caacgcgacg gcgagagctg gcggatcatc aacatcgtcg ccgacggcgt
      661 cagcgatctc gcgctgaaac gcgccgaata tcagcgcgtg ttcgccgccg gcggaatcga
      721 cggcctcatc gccgagctcg agcagcagac gcagcggctc gagcgcggct agggatcagt
      781 tgttcgagcg ctcgggagtg cgatcgtggc tcgcgtcgcc gtggctcgcg tcgccgcccg
      841 actgcagacg ctgcgccgtc gcgagatgtt gccgcagcgt cggcagcaga tcctcggcga
      901 gctccgcgac cttcgagtcg ccgctctgag cctgcttctc gtatttcgcg atttcttcct
      961 gatggccctt gaccatctcc gccacgaact gccggtcgaa ctccgctccg gacagacgag
     1021 ccaacgcatc gtgcttcgcc gtttgctcgg gcgtcggctg cgtcggcacg ggcacgttca
     1081 tctccttcgc gagatcggcg gtcttctttt gcgccttcgt gtgatcctcg acgagcatct
     1141 cgccgaaatc acggacgccc tcgctctggc ctttcatctg cgcgagctcg cccattttga
     1201 tttcggcgat gtcgccgcgg atcgcatcca tcagaaacgt cgactccgga gtgttcgtcg
     1261 ccagcgcgac ttcttgcgcc gacgcaatgc cgcaggcgca taacaccaac agcgtaccca
     1321 ctgccgcgaa ggttttttcc gtaactcgtg ccatggccat tcctccgtga acggttgaga
     1381 gaacgagcgg gaaatgtgca agtcccgtac cacggggcgc gctttccggc gcccggcgcc
     1441 gggaaccggt cgggaaacgc cctattgggg cagtggcgcg aggcccaggt ggcgccggat
     1501 cgacgggcgc acggcgtcga cgttcgcgag gatcaggccc tgcaggcgct tgatgccttc
     1561 cgcgcgatcc gccacgggct cgtcgagcca gacggcgccc gacgatacga gctcgggttt
     1621 cttcgcggtc agcgtgtaaa tcgcgagatg cacgacggcg atcagctcgt cggcgccggg
     1681 tccgctcggc ctcttcgcga acttctgcat gtcggcgaat gtgcggctcg gcacttcggc
     1741 acgcacggct tcgagcgagg agccttcgcc gacggccgcg ggccgcacca tcagaaccgc
     1801 ttgaacgccg cgatcggaga gcgctttgag gaactcgtcc gtgtcgaggc tcttgacgtc
     1861 gctcgtaatg tcgtagctcg tcacggcgtc gtagtcgtgt gccttcgcta tcgccacgag
     1921 ctcgctctcg aagctctgtc gcagcgcggc gtcctcggtc aacgcgacga cggcggactt
     1981 gtaagtcgtg gctgtctggg ccgacgcgct cgccgcggcc agcgatgcac taacgagcag
     2041 caaggcggac cgcttgagca tgctctcaac cctcaacgca cgtagcggta gtactcgatg
     2101 ccttcgtcgc tgaagttcgg attcgcgctg ctgccgatcg actgaatcac gaagccctgc
     2161 tcgggatccc gcaggccttg cgcagtgccc gtatcgtgca ggctctcgta ctgcgcttgc
     2221 gtcagatcct gatgggcctt gagcgccgct tcgaagccga tcttcgccgc cgcgtcgcgc
     2281 cattcgcgcg cgaccgtcat cggaatggcc tcggcggcgt cgccgctgcc gtagccaacc
     2341 gcgagcactt tgcgcccgcc caagtcggcg ccgcgtctgt gagcgtcctc gaggccggcc
     2401 gccatccagg ccggcagagc cgcgcaatag acgttgccga tgtctgtcat cggctcggag
     2461 ccgagcgaca tcttggaggc gacgagagct ccgaactcct tgctgccgcg gaactccttc
     2521 aacagctgga tactgagcgg gtacgcgtcg gcgtcgatgt tgccgtcctt gacgagctgc
     2581 aggatgttcg gttgcgagcg catttcctcg agcaagcccg cgacgggcat attggcccga
     2641 gcgcagagct cctcgagctc ggcgagcccg cgcgtgccgt cctgcgccaa gccgaacaga
     2701 tagctcatcg caaacgactg ctcgggcatg cggtgatacg gccgatgcat gaagatcgcg
     2761 gcgagctcgc gatagtagtc cgacggctcg cgccccatgc gccgcgccat gtcgttcagc
     2821 gcgtgcagag tctcgtcgag atagcaggtc gtcgagtatt tgccgttgaa caccggcaaa
     2881 tcctggaaat ggcagttcaa cttgccgcgg atgatgttgc gcagcacggg cttgcgaaaa
     2941 tcgacggccc gatacgacga ggcgctgccg atgtgctcga gatgaacctt caacaagcgc
     3001 ggattgcgct cgacgagcat tgcgacggcg ccggcgcctt gcgtcggctc gccggagctg
     3061 ccgcgcgcgt actccgcgat gtcggcgctg acgacgatcg cgcagcggtc ctcgggctcg
     3121 agcgccagat aacgcagcgc gtgcttgagc gcgtagacgc cgcccaagca ggcgtgcttg
     3181 acctcgggca cttcgcagtg acgcgagagc ggggcgcggc cgctcgcctg caggccttcg
     3241 tcgagcatgc ccttgacgat gacggcaccg gccgagttat cggtgctcga ctcggtgccc
     3301 aatcccaaga aaccgacgcg ggcaggatcg atgtcatagc gatcgatgag gcgcatcacg
     3361 gccgtggcgg ccatcgtgta gacgctctgg gccgggccgc gcctccggaa gctgtggccg
     3421 acgactgcgc gtgtcttgtc ccaaacgttg ttggtccagc gacaccatga ctcgagattt
     3481 acacggtaag gcggcacata gacggcaaag ccgctaatac ccacttcttc ggtcatacaa
     3541 ctcccagtct gtgccgcagt cgacccccgt tgctcgggcg ccaccgtctc cggcccgcgc
     3601 gcgattatat gacaaacaga tggcaagcac ttctcgcgcc ccattggg
//
LOCUS       DASSNN010000491         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1216115, whole genome shotgun sequence.
ACCESSION   DASSNN010000491 DASSNN010000000
VERSION     DASSNN010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216115"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFL84_09100"
     CDS             complement(<1..576)
                     /locus_tag="VFL84_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HET7608819.1"
                     /translation="MIAEVVAVLAPVFVMAGIGYAWRLTGAPFDMAFVTRLIMYVAGP
                     CLVFTSLSRLALPLAQFWGMVGAMIVVTLGVAAISAALIKILKLPLRSFLPALTLGNT
                     GNLGLPLCLFAFGEEGLGLAVAVYVTNSLLQFTFVPLLHSRVSVVRTLFSTPMLYGAA
                     AGLAVLIAEVRVPVWLANTLGTLGDLLIPLML"
     gene            642..1400
                     /locus_tag="VFL84_09105"
     CDS             642..1400
                     /locus_tag="VFL84_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608820.1"
                     /translation="MNRPSLTLRTLGSSMLGQFLEYSVAARPLAASFEFFESLGFKSI
                     PVSDTLPDPYVVCFDGTIAIGLHDRAQSGPRLTFVRPGLRHYVRPLRRLGVELTSEHL
                     RDNEFNSVAFNDPGGQEIALIEARTFPPGNWDAHNVAICGELLEVTLPAEDLEASSRL
                     WQAFGLTVAASGDVPHRWQRLTGHGVTLGLHETHCAAGLSFRCEDLDARLEYLRAKGL
                     SARDGSPIADRAQPSATLTGPEKTHLYLFEKRAQ"
     gene            complement(1407..1985)
                     /locus_tag="VFL84_09110"
     CDS             complement(1407..1985)
                     /locus_tag="VFL84_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019021709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET7608821.1"
                     /translation="MPLEWIDYRVAATLYHSDQVAYACGSPLYRIRGGYSAKSGNRSV
                     IEVNSIIATHGDTHTLERNRERYTPPLNNRTLFRRDANLCMYCGLQFRASELTRDHIT
                     PISRGGRDVWTNVVTACRRCNNHKGGRTPEEARLQLVAVPFTPTYAEYIYLKGRRVLA
                     DQMQYLLTHFPRSSPLHARLRDALAEVNRKAS"
     gene            2221..>2499
                     /locus_tag="VFL84_09115"
     CDS             2221..>2499
                     /locus_tag="VFL84_09115"
                     /inference="COORDINATES: protein motif:HMM:NF017072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HET7608822.1"
                     /translation="MPLSIGVLKESFVHEARVALTPDVAAKFLELGVGIHMERGAGER
                     SHLGDELYKGVQFHGDAAAVLAASSVLLKVQPPQLTEVAALPAGAVVVG"
BASE COUNT          443 a          818 c          835 g          403 t
ORIGIN      
        1 gagcatgagc gggatcagca agtcgccgag cgtgccgagc gtgtttgcga gccagaccgg
       61 cacacggacc tcggcgatca agacggcgag accggcagcg gcgccgtaca gcatcggcgt
      121 cgagaacagc gtccgcacga ccgatacgcg gctgtgcagt agcggcacga acgtgaactg
      181 cagcaacgag ttcgtcacgt aaacggcgac ggcgaggccg agcccttcct cgccgaacgc
      241 gaacaggcag agcggcaatc cgaggttgcc ggtattgccg agcgtcaacg cgggcaagaa
      301 cgaacggagc ggcaacttga ggatcttgat cagcgccgcc gagatggccg cgacgccgag
      361 cgtcacgacg atcatcgcgc ccaccattcc ccagaactgc gcaagcggaa gcgcgagacg
      421 cgacaggctc gtgaacacga ggcacggtcc cgcgacgtac atgatgaggc gcgtcacgaa
      481 cgccatgtcg aacggcgcac ccgtcaggcg ccacgcgtaa ccgatcccgg ccatcacgaa
      541 caccggcgcg agaacggcga caacttcggc gatcacgagc gggccttcac ggtgcgatcg
      601 gtccgtatcg aggacgacgc ggcgcggcga agtctgccag aatgaaccgc ccaagtctca
      661 cgctgaggac tctcgggtcg tcgatgctcg gtcagttcct ggagtattcg gtcgcggctc
      721 ggccgctcgc cgcttcgttc gagttcttcg agtcgctggg cttcaagagc atcccggtca
      781 gcgacacgct gcccgacccc tacgtagtct gcttcgacgg caccatcgcg atcgggctgc
      841 acgaccgcgc gcagtccggg ccgcggctca cgttcgtgcg cccgggcctg cgccactacg
      901 tgcggcctct tcgccgcctc ggcgtcgagc tcacgagcga gcacttgcgc gacaacgagt
      961 tcaactccgt cgctttcaac gacccgggcg gccaagaaat cgcgctgatc gaggcgcgca
     1021 cctttccgcc gggtaattgg gatgcgcaca acgtggcgat ctgtggcgag ctactcgagg
     1081 tcacgttgcc ggccgaggac ctcgaggcgt cgagccgctt gtggcaggcg ttcgggctca
     1141 cggtcgccgc ctccggcgac gtcccgcatc ggtggcaacg gctgacggga cacggcgtga
     1201 cgctcgggtt gcacgagacg cactgtgcgg cgggcctcag ctttcgctgc gaggacttgg
     1261 acgctcgcct cgagtatctg cgcgcgaagg gattgtcggc gcgcgacggg tctccgatcg
     1321 cggaccgcgc gcagccgtcc gcgacgctga cgggcccgga gaaaacgcac ctctacttgt
     1381 tcgagaaacg agcgcagtag gccgcgttac gacgccttgc gattgacctc ggccagcgca
     1441 tcgcgcagcc gcgcatgcag cgggctcgag cgcgggaagt gcgtcagcag gtactgcatt
     1501 tggtcggcga gcactcgacg gcccttcaaa tagatgtact cggcatacgt cggcgtgaac
     1561 ggtacggcga cgagctggag gcgcgcttcc tcgggcgtcc gcccgccctt gtgattgttg
     1621 cagcgccggc aagccgtcac gacgttggtc cagacgtcgc ggccgccgcg gctgatcggc
     1681 gtgatgtgat cgcgcgtgag ctccgaggcc cggaattgca ggccgcagta catgcaaagg
     1741 ttcgcgtcgc gcctgaacaa cgtgcggttg ttcagcggcg gcgtgtagcg ctcgcggttg
     1801 cgctcgagcg tgtgcgtgtc gccgtgcgtc gcgatgatcg agttcacctc gatgacgctg
     1861 cggttgcccg acttggcgct gtagccgcca cggatgcggt acaacggcga gccgcaggcg
     1921 taggccacct gatcgctgtg gtacagcgtg gcggcaacgc ggtaatcgat ccactcgagt
     1981 ggcatccccg cgacatcagt acgaagaact tcttgagcga gtgtgtgcac aggtccttgc
     2041 atccagacac catccaggcc gctaggacat ctcaagtttc accgagagat caccaagatt
     2101 caatggccta cgtgacaccc ttgtgtcatc atcgtgggcc aagaggctag ccgattcccg
     2161 cttgtcgcgt tgccccgccg caacgcgcta ccgaaaaaac cgcgagcacg gagccgacgg
     2221 atgccactca gtatcggggt gctgaaggag tcctttgtcc acgaggcgcg cgtcgccttg
     2281 acccccgacg tcgccgccaa gtttctcgag ctcggcgtcg gcattcacat ggagcgcggg
     2341 gccggggagc gcagccatct cggcgacgag ctgtacaaag gcgtgcagtt ccatggcgat
     2401 gcagccgccg tgctcgccgc gtcgtccgtt ctactgaagg tccagccgcc gcagctcacc
     2461 gaggtcgcgg cgttgccggc cggcgccgtc gtcgtgggc
//
LOCUS       DASSNN010000492         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_215100, whole genome shotgun sequence.
ACCESSION   DASSNN010000492 DASSNN010000000
VERSION     DASSNN010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215100"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /gene="coaE"
                     /locus_tag="VFL84_09120"
     CDS             complement(<1..409)
                     /gene="coaE"
                     /locus_tag="VFL84_09120"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.4"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET7608823.1"
                     /translation="MADDSKLGPRLVVGLTGGIASGKSLVGAMFVKLGAPLIDTDVVA
                     REVVAVGEPGLAAVTAEFGPGVLLPSGELNRPGLRSMVFADDEKRRRLEAILHPLIRA
                     RTRSKLAELEAPYALVAVPLLVETNFGELVDRIL"
     gene            complement(469..1320)
                     /locus_tag="VFL84_09125"
     CDS             complement(469..1320)
                     /locus_tag="VFL84_09125"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET7608824.1"
                     /translation="MALLVVAGLAGLAVGSFLNVVAYRLPVMMERAWRAHCEELKSQP
                     FEAPAHIAERQRFDLWWPRSACPGCGNPIAAQHNIPVVGFLWLRGKCFSCGAKISRRY
                     PIVEALAAVLGLVTAYVLGPTWQTVAALGFAWTLLALTLIDLDHKLLPDSLTLPLLWA
                     GLLVNVPGLFTTLHSSVLGAAAGYLALWSVYQLFKLVTGKEGMGYGDFKLLAALGAWL
                     GWQLLPVVILLSAVVGSVVGIALIVFGGRSRQAAIPFGPYLAAAGFIALLWGERLVQL
                     YQAWFFR"
     gene            complement(1370..>2332)
                     /locus_tag="VFL84_09130"
     CDS             complement(1370..>2332)
                     /locus_tag="VFL84_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018868663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7608825.1"
                     /translation="VQSFDIVGAGHENPAMQKLILAIKADVEGGTSLHEALAKHPLHF
                     DDLFVSLVAAGEQAGALETLLDKIATYKEKTEAIKKKIKKALFYPCAVVVVAIVVTAI
                     LLIYVIPEFESLFQGFGADLPAFTRFVIDISKFVQSKGWILLVMVVGGIWGLIEAKKR
                     SRGVQHFFDRALLKLPILGAILNKSAIARYARTLSTTFAAGVPLVEALSSVAGACGNV
                     VYESAVIKMRDEVATGQRLQRAMENTNLFPNMVNQMVAVGEESGSLDQMSAKVADFYE
                     EEVDNAVDSMSSLLEPLIMAILGVLVGGLVIAMYLPIFKMGAVV"
BASE COUNT          440 a          781 c          761 g          350 t
ORIGIN      
        1 cgaggatccg gtcgacgagc tcgccgaaat tcgtctcgac gagcaacggc accgccacga
       61 gcgcgtaagg cgcctcgagc tccgcgagct tcgagcgcgt gcgcgcgcgg atcagcggat
      121 gcaggatcgc ctcgagcctg cggcgtttct cgtcgtccgc gaagaccatc gagcggagcc
      181 ccggccggtt cagctcgccc gagggcagca gcacgccggg cccgaattcg gccgtgacgg
      241 cggcgaggcc gggctcgccg actgcgacga cctcgcgcgc cacgacgtcg gtgtcgatga
      301 gcggcgcgcc gagcttcacg aacatcgcgc cgacgagcga cttgccgctc gcgatgccgc
      361 ccgtcaagcc cacgacgaga cgcggaccta acttcgagtc gtccgccatg cggtagctct
      421 ctgcaactta gggtctcgcc acgccgagat tttgcaggcg cgaaagcctc aacggaagaa
      481 ccacgcctgg tagagctgca cgagccgctc tccccacagc agggcgatga atcccgcggc
      541 ggcgaggtag ggcccgaaag ggatagcggc ctggcgcgag cgtccgccga aaacgatcag
      601 cgcgatgccg acgacgctgc cgacgacggc ggagagcagg atcacgacag gcaacagctg
      661 ccatccaagc caggcgccga gcgcggccag cagcttgaag tcgccgtagc ccatgccctc
      721 tttgcccgtc acgagcttga acagctgata gacgctccag agcgcgaggt agcccgccgc
      781 cgcgccgagc acgctcgagt gaagcgtcgt gaatagcccc ggcacgttga cgagcagccc
      841 cgcccagagc agcggcagcg tcagcgaatc cggcagcagc ttgtgatcga ggtcgatcag
      901 cgtcagcgcg agcagcgtcc acgcgaagcc gagcgccgcg accgtctgcc atgtcggccc
      961 gagcacgtac gccgtcacga ggccgagaac ggccgccaac gcctcgacga tcggatagcg
     1021 ccgcgagatc ttcgcgccgc agctaaagca tttgcccctg agccagagga acccgacgac
     1081 gggaatattg tgctgcgccg caatcgggtt gccgcagccg gggcacgccg accgaggcca
     1141 ccagaggtcg aaccgctgcc tctcggcgat gtgcgcgggg gcttcgaaag gctgcgattt
     1201 cagctcctcg cagtgcgcgc gccaggcgcg ctccatcatg acgggtaagc gatacgcgac
     1261 gacgttcagg aagctgccga cggcgagacc ggccaggccc gcgacgacga gcaacgccac
     1321 gggccaagcc tcgaagatct ccactagtat cggcgccgcg cggggcgctt cagacgacgg
     1381 ctcccatctt gaagatgggc aggtacatcg cgatgacgag accaccgacg agcacgccga
     1441 ggatcgccat gatcagcggc tcgagcaagc tcgacatgct gtcgacggcg ttgtcgactt
     1501 cttcctcgta gaagtcggcg actttggccg acatctggtc gagcgagccc gactcttcgc
     1561 cgacggcgac catctggttg accatgttcg ggaacaggtt cgtgttctcc atggcgcgct
     1621 ggaggcgctg gcccgtcgcg acctcgtctc gcatctttat caccgcgctt tcgtagacga
     1681 cgttgccgca cgcgccggcg accgagctga gcgcctcgac gagcggcacg ccggccgcga
     1741 aggtcgtcga gagcgtgcgc gcgtagcgcg cgatcgcgga cttgttgagg atcgcaccca
     1801 agatcggcag cttcagcagc gcgcggtcga agaagtgctg gacgccgcga ctgcgcttct
     1861 tggcctcgat gagcccccag atgccaccga cgaccattac gagcagaatc cagcccttgc
     1921 tctgcacgaa cttcgagatg tcgatgacga agcgcgtgaa cgcgggcaaa tcggcgccga
     1981 agccctggaa cagcgattcg aactcgggaa tgacgtagat cagcaggatc gccgtgacga
     2041 cgatcgcgac gacgacgacc gcgcacgggt agaacagcgc cttcttgatc tttttcttga
     2101 tggcctcggt tttttccttg taggtcgcga tcttgtcgag cagcgtctcg agcgcgccgg
     2161 cctgctcgcc ggccgccacg aggctcacga acagatcgtc gaaatgcagc ggatgcttcg
     2221 cgagcgcctc gtgtagcgag gtaccgcctt cgacatcggc cttgatcgcc aagatcagct
     2281 tctgcatcgc gggattttcg tggccggcgc cgacgatgtc gaacgactgc ac
//
LOCUS       DASSNN010000493         2458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1833053, whole genome shotgun sequence.
ACCESSION   DASSNN010000493 DASSNN010000000
VERSION     DASSNN010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833053"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFL84_09135"
     CDS             <1..501
                     /locus_tag="VFL84_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7608826.1"
                     /translation="RLMIARALVHEPRLLILDEPTAGVDIEIRRSMWEFMRDMNAKGT
                     TIILTTHYLEEAESLCRNVAIIDEGRIIENDAMANVIGKMQLEVFVLSLLEPVKAAPE
                     LSGYTVRLRNEREIEVEVAKGQGLNDVFAQLSAQGLHVSSMRTKTNRLEELFIRLVDR
                     KERKSA"
     gene            498..1310
                     /locus_tag="VFL84_09140"
     CDS             498..1310
                     /locus_tag="VFL84_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008897222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7608827.1"
                     /translation="MSVAIERTQARDLNLWRINLVGLWTIVRKETRRVVRIWIQTIVP
                     PAITMTLYFIIFGSLVGRRIGSMDGVPYMTFIAPGLIMMAVITNSFANVVSSFFSSKL
                     MLHLEEMLVAPLAHTTIVLGFVVGGVVRGLLVAALVVVVALFFTDLHIAHPLITFTTI
                     LLTAVVFSTAGLFNAIFAKTFDDISIIPTFVLTPLTYLGGVFFSIELLPPFWKHVAQL
                     NPILYMVNAFRFGMLGSSDISLGVAYAIMLAAAAGLFGAVLFMLKRGIGVRA"
     gene            1314..1997
                     /locus_tag="VFL84_09145"
     CDS             1314..1997
                     /locus_tag="VFL84_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HET7608828.1"
                     /translation="MCGRFGLFVTPEVLEEYFSLERVEGAAAPAPRYNLTPGQAVAVV
                     REHEGRRRLDALQWGLIPFWAKDATIGRRLVNARLDSVAAKPAFREAWQRRRCLIPAS
                     GFYEWSEPRGGRKRPYFIRPSDEPLLAIAGLWERWRTPSGEKLETCVIVTTDATPTLV
                     KIHDRMPLLIPRDAQALWLDPKSSLDDVRTLTERPPALDIHAVGFGVNDPKNDDEALI
                     APVEEAAQG"
     gene            complement(2004..>2458)
                     /locus_tag="VFL84_09150"
     CDS             complement(2004..>2458)
                     /locus_tag="VFL84_09150"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HET7608829.1"
                     /translation="PPEASVPTVERRVDLPPPAPRSSAIQQAAPSRLSDSSLSAAAAS
                     STGESNQSAAEPAAPGLSAPPRLTNLPDDDVALPSVAMLAAEGIAVPPLHLELHAFSP
                     QPRDRFVFINGRKYVEGDRLVEGPMLVAIQPTGAVLTHAGRRFVLVQE"
BASE COUNT          395 a          810 c          822 g          431 t
ORIGIN      
        1 cggctcatga tcgcgcgcgc gctcgtccac gagccgcgcc tgctgatcct cgacgagccg
       61 acggccggcg tcgacatcga gatcagacgc tcgatgtggg agttcatgcg cgacatgaac
      121 gcgaagggca cgacgatcat cctgacgacg cattatctcg aggaagccga gagcctttgc
      181 cgcaacgtcg ccatcatcga cgaggggcgg atcatcgaga acgacgcgat ggcgaacgtc
      241 atcggcaaga tgcagctcga agtgttcgtg ttgagcttgc tcgagccggt caaggccgca
      301 cccgagcttt cgggctacac ggtgcgcttg cgcaacgagc gcgagatcga agtcgaggtc
      361 gcgaaggggc aggggttgaa cgacgtgttc gcgcagcttt cggcccaagg cttgcacgtc
      421 tcgagcatgc gcaccaagac gaaccggcta gaggagctgt tcattcggct cgtcgacaga
      481 aaggagcgga aatccgcatg agcgtcgcga tcgagcgcac gcaagcgcgc gacctgaatc
      541 tttggcgcat caatctcgtc ggcttgtgga cgatcgtgcg caaggagacg cggcgcgtcg
      601 tgcggatctg gatacagacg atcgtgccgc ccgcgatcac gatgacgctg tacttcatca
      661 tcttcggcag cctcgtcggc cggcgcatcg gctcgatgga cggcgtgccg tacatgacgt
      721 tcatcgcccc gggcctcatc atgatggccg tcatcacgaa ctcgttcgcg aacgtcgtat
      781 cgtcgttctt ctcgagcaag ctcatgctgc acctcgagga gatgctcgtc gcgccgttgg
      841 cgcatacgac gatcgtgctc ggtttcgtcg tcggcggcgt cgtgcgcggt ttgctcgtgg
      901 cagcgctcgt cgtcgtcgtc gcgctgttct tcacggatct gcacatcgcg catccgttga
      961 ttacgttcac gacgatcctg ctgacggccg tcgtcttctc gaccgcgggc ctcttcaacg
     1021 cgatctttgc gaagacgttc gacgacatct cgatcatccc gacgttcgtg ctgacgccgc
     1081 tgacctatct cggcggcgtg ttcttctcga tcgagctgct gccgccgttc tggaagcacg
     1141 tcgcgcagct gaacccgatc ctctacatgg tcaacgcgtt ccgcttcggc atgctcggct
     1201 cttccgacat ctcgctcggc gtcgcctacg cgatcatgct cgccgccgcc gcgggtctct
     1261 tcggtgccgt gctgttcatg ctcaagcgcg gcatcggcgt cagagcgtga cgtgtgtgcg
     1321 gccgattcgg attgttcgtc acgcccgagg tgctcgagga gtacttcagc ctcgagcgcg
     1381 tcgaaggcgc ggccgccccg gcgccgcgct ataacctgac cccgggtcag gccgtcgccg
     1441 tcgtgcgcga gcacgagggc cggcgccggc tcgacgctct gcagtggggc ttgatcccgt
     1501 tctgggcgaa agacgccacg attggccggc gcctcgtcaa cgcgcgcctc gacagcgtcg
     1561 ccgccaagcc ggccttccgc gaagcgtggc agcggcgccg gtgcctgatt cccgcgagcg
     1621 gcttctacga atggagcgag ccgcgcggcg gccgcaaacg cccctacttc atccggccga
     1681 gcgacgagcc gctgctcgcg atcgcgggct tgtgggagcg atggcgcacg ccgtcgggcg
     1741 agaagctcga gacctgcgtc atcgtcacga cggacgctac acccacgctc gtgaagattc
     1801 acgaccgcat gccgctcttg attccgcgcg acgcgcaggc gctgtggctc gatcctaaga
     1861 gcagcctgga cgacgtgcgc acgctcacgg agcggccgcc tgcgctcgac attcatgccg
     1921 tcggattcgg cgtcaacgat ccgaagaacg acgacgaagc gctgatcgcg cccgtcgagg
     1981 aagccgcgca aggttgagcg acgctactcc tgaacgagga cgaaccggcg accggcgtgc
     2041 gtgagcacgg ccccggtcgg ctggatcgcg acgagcatcg ggccctcgac gagccggtcg
     2101 ccttcgacat atttgcggcc gttgatgaac acgaagcggt cgcgcggctg cgggctgaag
     2161 gcgtgcagct cgaggtgcag cggcggcacg gcgatgcctt cggcggcgag catcgcgacg
     2221 ctcggcagcg caacgtcgtc gtcgggcaag ttcgtgagcc gcggaggcgc cgagagcccg
     2281 ggcgctgcgg gctccgcggc gctctggttc gattcgcccg tgctcgacgc ggccgccgcc
     2341 gaaagcgacg agtcggacag ccgcgacggc gcggcttgtt gaatcgcgga gctgcgcggc
     2401 gccggcggag gcagatccac tctgcgctcg accgtcggga ctgacgcctc cggcggcg
//
LOCUS       DASSNN010000494         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_319352, whole genome shotgun sequence.
ACCESSION   DASSNN010000494 DASSNN010000000
VERSION     DASSNN010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319352"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="VFL84_09155"
     CDS             <1..1384
                     /locus_tag="VFL84_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7608830.1"
                     /translation="TANRYKVPLYPISTGRNLGYGGAAPAYSGCVVVDLKRMNRILDV
                     SERDKTCLVEPGVSYFDLYRHLRTNKIPLWIDTADPGWGGLIGNALDRGGGYTDVEFR
                     DHFDAHCGMEVVLPTGEVVRTGMGANPQAKTWQLFKYGLGPWIDGIFSQSNYGIVTKM
                     GFWLMQEPEAALQVSVTVPKRRDVVPLVDTLHSLMCAHTVPSQVTVASPLMAGNNPEL
                     VRLRGSGGASDADWERFAASAGGAPFWAATFVYYGPRKVIAAQWEHTKERLSGIAGVQ
                     FRETASHTFPLTDEQVEAVADKARYGIPSLNLFSSRSAPGAPPLEGHIDFSPMIAPHG
                     EDLIALTDYTGKVYADQGFTPSFVAGMMFHPRTMICFHAVPTFRNAADNARTRKLFES
                     LVRGCAERGWPIYRIHPVFQKLGMEMFGWGGGSLHRLHETLKDAIDPNGIVSPGRYDI
                     WPKRLRGTRA"
     gene            1381..1761
                     /locus_tag="VFL84_09160"
     CDS             1381..1761
                     /locus_tag="VFL84_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7608831.1"
                     /translation="MKHIGLVALLVAGAVTAQDAPFPIAAWSRTTAAVETQGSPVERG
                     AAVFNNWCSACHSRGPQNAPGTASLQAKYQGSVPAALEDRRDLTPDLVKFFVRNGVAM
                     MAPLRKTEVSDADLEALAAYLTQR"
     gene            complement(1821..>2196)
                     /locus_tag="VFL84_09165"
     CDS             complement(1821..>2196)
                     /locus_tag="VFL84_09165"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7608832.1"
                     /translation="AATAFAVLATALAGLGLYSVLAYTVAQRSREIGLRFALGAPAGR
                     IRGMLLRQMSAVTIFGIAFGAAAAWLLGRAARSLLFGVEAADPIALAGAAIVLVAVTL
                     GAAYLPARRASRVDPIVVLRYE"
BASE COUNT          355 a          749 c          760 g          332 t
ORIGIN      
        1 caccgcgaac cgctacaagg tgccgctcta tcccatctcc accgggcgca atctcggcta
       61 cggcggggcg gcgccggcgt attcgggctg cgtggtcgtc gacctgaagc gcatgaaccg
      121 catcctggac gtcagcgagc gcgacaagac ctgcctcgtc gagcccggcg tcagctactt
      181 cgatctctac cggcacctgc gcacgaacaa gatcccgcta tggatcgaca cggccgatcc
      241 tggctggggc ggcctcatcg gcaacgcgct cgaccgcggc ggcggctaca cggacgtcga
      301 gttccgcgat cacttcgacg cgcattgcgg catggaagtc gtgttgccga cgggcgaggt
      361 cgttcgcacc ggcatgggcg cgaatccgca ggcgaagacg tggcagctgt tcaagtacgg
      421 cctcggccct tggatcgacg gcatcttctc gcaatcgaac tacggcatcg tcacgaagat
      481 gggcttctgg ctcatgcagg agcccgaagc cgcgctgcaa gtcagcgtga cggtgccgaa
      541 gcgccgcgac gtcgtgccgc tcgtcgacac gctgcatagc ttgatgtgcg cgcacacggt
      601 cccgtcgcag gtcacggtgg cgagcccgct gatggcgggc aacaacccgg agctcgttcg
      661 gctgcgcggc tcgggcggcg cgtccgacgc cgactgggag cggttcgcgg cgagcgccgg
      721 aggcgcgccg ttctgggccg cgacgttcgt ctactacggg ccgcgcaagg tcatcgccgc
      781 gcagtgggag cacacgaagg agcgcttgtc cggcatcgcc ggcgtccaat tccgcgagac
      841 tgcgtcgcat acgttcccgc tgaccgacga gcaggtcgag gccgtcgccg acaaggcgcg
      901 ttacggcatc ccgtcgctga atctgttcag ctcgcgttcg gcgcccggcg cgccgccgct
      961 cgaaggtcac atcgatttct cgccgatgat tgcgccgcac ggcgaagact tgatcgcgct
     1021 gacggactac acgggtaagg tctacgccga tcagggtttc acgccgtcgt tcgtcgccgg
     1081 gatgatgttc catccgcgca cgatgatctg cttccatgcc gtgccgacgt tccgcaacgc
     1141 ggccgacaac gcgcgcacgc gcaagctgtt cgagtcgctc gtgcgcggtt gcgccgagcg
     1201 cggctggccg atctaccgca ttcatcccgt gttccagaag ctcggcatgg agatgttcgg
     1261 ctggggcggc ggttctctgc accggttgca cgagacgctg aaggacgcga tcgatccgaa
     1321 cgggatcgtc tcgccggggc gctacgacat ctggccgaag cggctgcggg ggacgcgggc
     1381 atgaagcaca tcggcctcgt cgcattgctc gtcgccggtg ccgttacggc gcaggacgcg
     1441 ccgttcccga tcgcggcgtg gagccgcacg acggccgccg tcgaaaccca aggctcgccg
     1501 gtcgagcgcg gagcggccgt cttcaacaac tggtgcagcg cctgccactc gcgcgggccg
     1561 cagaacgcgc cgggcacggc gtcgctgcaa gcgaagtatc agggcagcgt gccggccgcg
     1621 ctcgaggacc ggcgcgatct gacgcccgat ctcgtgaagt tcttcgtccg caacggcgtc
     1681 gcgatgatgg cgccgttacg caagaccgag gtcagcgacg cggacctcga ggcgctcgcg
     1741 gcgtacctga cgcagcgtta gatcccgccg tcgagcgatt cggcgagcag cgcgtcgtcg
     1801 aacaccgtga gcctcgttgg ctattcgtag cgcagcacga cgatcgggtc gacgcgcgaa
     1861 gcgcgccgcg ccgggaggta ggcggcgccg agtgtgaccg caacgagcac gatcgccgcg
     1921 ccggccagcg caattgggtc ggcggcctcg actccgaaca acaggctgcg cgccgcgcgc
     1981 ccgagcagcc acgcggccgc ggcgccgaat gcgatgccga agatcgtcac ggcgctcatc
     2041 tgtcgaagca gcatgccgcg tatgcggccc gccggcgcgc cgagcgcgaa ccgcaagccg
     2101 atttcgcgcg agcgctgcgc gaccgtgtac gcgagcacgc tgtagaggcc gaggccggcc
     2161 agcgcagtgg cgagcaccgc aaacgcggta gccgct
//
LOCUS       DASSNN010000495         3110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_319359, whole genome shotgun sequence.
ACCESSION   DASSNN010000495 DASSNN010000000
VERSION     DASSNN010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319359"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFL84_09170"
     CDS             complement(<1..207)
                     /locus_tag="VFL84_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378451.1"
                     /note="GTPase; similar structure to tubulin; forms
                     ring-shaped polymers at the site of cell division; other
                     proteins such as FtsA, ZipA, and ZapA, interact with and
                     regulate FtsZ function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET7608833.1"
                     /translation="MFELMDGYSQTAVIKVLGIGGGGGNAVAHMVRQGIEGVDFICAN
                     TDAQALKTAKVKTNMQIGCNITKGL"
     gene            complement(242..1477)
                     /gene="ftsA"
                     /locus_tag="VFL84_09175"
     CDS             complement(242..1477)
                     /gene="ftsA"
                     /locus_tag="VFL84_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET7608834.1"
                     /translation="MGRRDDRKLIVGLDIGTSKVVAIVGELSAEDRLEVIGLGSNPSR
                     GLKRGVVVDIESTVQSIQRAIEEAELMAGCEINSVLTGIAGSHVRSLNSHGIVAIRDK
                     EVSAGDVERVIDAARAVAIPADQRVIHVLPQEFIIDSQDGIREPIGMSGVRLEAKVHL
                     VTGAVSAAQNVVKCVQRCGLTVEDVVLEQLASSYAVLTEDERELGVCLVDMGGGTTDI
                     AIFSGGAIQHTAVIPIAGDQVTNDIAVSLRTPLPYAEEIKIKYACALSQLANPDETIE
                     VPSVGDRPPRRLARQTLAEVVEPRYEELFQLIREELRRSGFEELMAAGIVLTGGSAKM
                     EGAIELAEEVFHMPVRLGVPQFVEGLSDVVRNPIHATGVGLLLYSKQALEAKAEATAS
                     RLGFSNVFERMRAWFQKNF"
     gene            complement(1508..2320)
                     /locus_tag="VFL84_09180"
     CDS             complement(1508..2320)
                     /locus_tag="VFL84_09180"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HET7608835.1"
                     /translation="MFGRRANRRRVESRLARSKLPTLGAAWLRRLRAALVVPLAALTL
                     YGAFKGIQLALDQPVRELVVEGTFQRVTPIQVEAAVAEGLDAGFMTVNLSTLRERVQQ
                     LDWVDRANVGRRWPDTLIVRVTEHQAAARWGETGLLNVRVELFTERAQHSFAELPSLS
                     GPPGTERDVARRYLAVRGKLADAGLTLESLELDERGAWRLVLGGGQEIRLGRRDIDER
                     FLRFFGVVAPALAADLKRVDYVDLRYTNGFAVGWREGPPEHLAAVAAIRTGG"
     gene            complement(2320..>3110)
                     /gene="murB"
                     /locus_tag="VFL84_09185"
     CDS             complement(2320..>3110)
                     /gene="murB"
                     /locus_tag="VFL84_09185"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET7608836.1"
                     /translation="RRILADLPAAIPVHWLGLGSNLLVRDGGIRGAVIATGSLARELE
                     RVDERRVRASAGLACMLLAKRCVRWQLGPAAFFAGIPGTVGGALAMNAGAFGGETWTH
                     VESVTTVDRGGEVRERPRAEFEISYRTVRGPANEWFLGATFRFDHDASSSMDAIKALL
                     ARRNAAQPLGVPSCGSVFRNPPGDFAGRLIEQAGLKGLRRGGAMVSDKHANFILNVDD
                     ATAADIEALIGEVQAAVQRASGVLLEPEVRVIGEPLGTQAKGDR"
BASE COUNT          477 a         1129 c          993 g          511 t
ORIGIN      
        1 gagtcccttc gtgatgttgc agccgatttg catattggtc ttgaccttcg cggtcttgag
       61 cgcttgggcg tccgtgttgg cgcagatgaa gtcgacgccc tcgattccct gccgcaccat
      121 gtgcgcgacg gcgttgccgc ctccgccgcc gataccgagc actttgatga cggccgtttg
      181 gctgtacccg tccattagct caaacatttc ctgatctcct caagtagtga atcgcgaagc
      241 cctagaagtt cttttgaaac cacgctctca tgcgttcgaa tacgttgctg aacccaagcc
      301 tcgacgccgt cgcttcggct ttggcctcga gggcctgttt gctgtagagc aagaggccga
      361 ccccggtcgc gtgaatcgga ttgcggacga cgtccgagag cccttcgacg aattgcggca
      421 cgcctaagcg caccggcatg tggaacacct cctcggcgag ctcgatcgcg ccttccatct
      481 tggcgctgcc gcccgtcagc acgatgccgg cggccatgag ctcctcgaac cccgagcggc
      541 gcagctcctc gcgaatgagc tggaacaact cctcgtagcg cggctcgacg acctcggcca
      601 gcgtctgccg cgcgagccgc cgcggcggcc ggtcgccgac gctcggcacc tcgatcgtct
      661 cgtcggggtt cgcgagctgc gacaacgcgc acgcgtactt gatcttgatc tcttcggcgt
      721 acggcaacgg cgtgcgcaag ctgacggcga tgtcgttcgt gacttgatcg ccggcgatcg
      781 gaatgacagc cgtgtgctgg atcgcgccgc cgctgaagat cgcgatgtcg gtcgtgccgc
      841 cgcccatgtc gacgaggcag acgccgagct ccctctcgtc ctcggtcagc acggcgtagc
      901 tcgacgcgag ctgctcgagc actacgtctt cgaccgtcaa gccgcagcgc tgcacgcact
      961 tcacgacgtt ctgtgcggcg ctgaccgcgc cggtgacgag atgcaccttc gcttcgagcc
     1021 gcacgccgga catgccgatc ggctcgcgga tgccgtcctg cgagtcgatg atgaactcct
     1081 gcggcagcac gtggatgacg cgttgatccg ccgggatcgc gacggcgcgc gccgcgtcga
     1141 tgacgcgctc gacgtcgccg gcactgacct ccttgtcgcg gatcgcgacg atgccgtgcg
     1201 agttcaggct gcggacgtgg ctgcccgcta tcccagtcag cacgctattg atctcgcagc
     1261 cggccatgag ctcggcttcc tcgatcgcgc gctggatcga ctgcaccgtc gactcgatgt
     1321 cgacgacgac gccgcgcttg agtccgcgcg acggattcga tcccaagccg atgacctcga
     1381 gccgatcctc ggccgacagc tccccgacga tcgccacgac tttcgacgtg ccgatgtcga
     1441 gaccgacgat gagctttcga tcgtctcgcc tacccacttg tcacgctccc ctgcacttcc
     1501 cctctcccta gccgcccgtg cgaatcgcgg cgacggccgc gagatgctcg ggcggccctt
     1561 cgcgccagcc gacggcgaac ccgttcgtgt agcgcaagtc cacgtagtcg acgcgcttca
     1621 gatcggccgc gagcgccggc gctacgacgc cgaagaatcg aaggaaccgc tcgtcgatgt
     1681 cgcggcggcc gagcctgatc tcctgcccgc cgccgaggac gagccgccaa gcgccgcgct
     1741 cgtcgagctc gaggctctcg agcgtcaagc cggcgtccgc gagcttgccg cgcacggcca
     1801 agtaacggcg cgcgacgtcg cgctccgtgc ccggcggccc ggacaagctc ggcagctccg
     1861 cgaacgagtg ctgcgcgcgc tccgtgaaga gctcgacgcg cacgttcagg agccccgttt
     1921 ctccccagcg cgcggcggcc tggtgctcgg tcacgcgcac gatcaacgtg tcgggccagc
     1981 gccggccgac gttcgcgcga tcgacccaat cgagctgctg cacgcgctcg cgcaacgtgc
     2041 tcaagttcac ggtcatgaag ccggcgtcga gcccttcggc cacggcggcc tcgacctgaa
     2101 tcggcgtgac gcgctggaac gtgccctcga cgacgagctc gcgcacgggt tgatcgagcg
     2161 cgagctggat gcccttgaac gcgccgtaca gcgtcaacgc cgcgagcggc accacgagcg
     2221 cggcgcgcag gcgccgcagc cacgccgcgc cgagcgtcgg caacttcgag cgggcgaggc
     2281 ggctctcgac tctgcggcga ttcgctctgc ggccgaacat caacggtccc ccttcgcttg
     2341 cgtcccgagc ggctcgccga tcacgcggac ttccggctcg agcaacacac cgctcgcgcg
     2401 ctgcacggcg gcctgcacct caccgatcag cgcttcgatg tccgcggcgg ttgcgtcgtc
     2461 gacgttcaag atgaaattcg cgtgcttgtc cgagaccatt gcgccgccgc gcctcagccc
     2521 cttcagaccg gcctgctcga tgaggcgccc cgcgaaatcg ccgggcgggt tgcggaacac
     2581 cgagccgcag cttgggaccc cgagcggctg cgcggcgttg cgcctcgcga gcagcgcctt
     2641 gatcgcgtcc atcgagctcg acgcgtcgtg gtcgaagcgg aacgtggccc cgaggaacca
     2701 ctcgttcgcg gggccgcgca cggttcggta gctgatctcg aactctgcgc gcggccgctc
     2761 gcgcacctcg ccgccgcgat cgacggtcgt gacgctctcg acgtgcgtcc acgtctcgcc
     2821 gccgaaggcg cccgcgttca tcgcgagcgc gccgccgacg gtgccgggaa tcccggcgaa
     2881 gaatgccgca gggcccagct gccagcgcac gcagcgcttc gcgagcagca tgcaggcgag
     2941 acccgcgctc gcgcgcacgc gccgctcgtc gacacgctcg agctcgcgcg ccaagcttcc
     3001 cgtcgcgatc acggcgccgc gaattccgcc gtcgcgaacg agcaagttcg accccaagcc
     3061 gagccaatgc actggaatgg ccgccggcag gtccgcgagg atgcggcgaa
//
LOCUS       DASSNN010000496         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1833945, whole genome shotgun sequence.
ACCESSION   DASSNN010000496 DASSNN010000000
VERSION     DASSNN010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833945"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1940
                     /locus_tag="VFL84_09190"
     CDS             135..1940
                     /locus_tag="VFL84_09190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET7608837.1"
                     /translation="MKSLPRSRRYLPGLVAALAWTAVALAQAAPETTYIVQSASLDSA
                     RMQVRRVGAEPERDLDIINAVAVRLTEAQVAELRETPNVRVYDDRTVSARGTLLGGLT
                     DLVNDLNSTLAENPLVQTVQTIASPVVSTLACNPLVSTVTSPLVDTLQGVQDGTSLAS
                     LPLIYETNYPAMIGADKLHCRGITGRGVTIAVLDTGLWRDTFQFYGNRILATVDVTYG
                     GRGPVTGDPYGHGTHVTSIAASGAQALSGGYQGIAPQANVVVVRAFNGTGAGRYVDVI
                     AGLNWIVQNRARHNIRVLNLSFGAAPQSFYWDDPLNQAVMAAWRAGIVVVASAGNDGP
                     DPMTVNVPGNVPYVITVGALTDSNTPYDYTDDKLASFSSTGPTFEGFVKPEVVSPGGH
                     IKASMSSSSFLASLDPKSVQATQQLFTMSGTSQAAAVTSGVVALMLQDEPWLTPDTVK
                     CRLMVAARPAVNTNGTLAYSVFQQGAGLINALDAVNSTASSCANRGLDIDDDLADRRH
                     FGGPANEDGNGGYYIMDMEGRRWGDSLDSDGYTWSRGYPWGEGYSWSQGYTWSRGYTW
                     SRGYTWSRSLSWWNAPATYIPMSKPASIATWVPNE"
     gene            2104..>2781
                     /locus_tag="VFL84_09195"
     CDS             2104..>2781
                     /locus_tag="VFL84_09195"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HET7608838.1"
                     /translation="MMGASAAAADDPALVLDHLTTEDGLPQATVMTTLQDSQGFVWLG
                     TEDGLVRYDGGELHRYARSRTEPDSLPGNYVWQVVEDADTNLWIALDGAGVAKWNRRT
                     DRFTSYRHDASRAASLASDRVRTVLVDGSGSVWVGTYDAGVDVLEPKSGNVEHLRHDP
                     DDLGSLASDRVSTLALDRAGDVWIGTDRGLNHWRAETRTMTRVGTPSGDARSLHDVEI
                     SRVLEDQS"
BASE COUNT          467 a          967 c          927 g          420 t
ORIGIN      
        1 cggctcacgt ttgacagtcg ccgttatgtg acggccgtca caaaaaccgg cgcgccgcgc
       61 gtcgggtccg gcagcgtcgc acggggccac ggcgcgttcc aatgcgcgcg ttagtcagcc
      121 gcgtgagaag aacgatgaaa agcctgcccc ggagccgccg gtatctaccc gggctcgtcg
      181 ccgcattggc ctggaccgcc gtcgcgctgg cgcaagcagc gcccgagacg acgtacatcg
      241 tgcagagcgc gtcgctcgat tcggcccgca tgcaggtccg ccgtgtcggc gcggagcccg
      301 agcgagacct cgacatcatc aatgcggtcg ccgtccgcct gaccgaggcg caggtcgcgg
      361 aattgcgcga gacgccgaac gtccgcgtct acgacgatcg cacggtcagc gcccgcggca
      421 cgttgctcgg cggtttgacg gatctcgtca acgacctgaa ctcgacactg gcggaaaacc
      481 cgctcgtaca gacggtgcaa acgatcgcga gccccgtcgt ttcgacgctc gcctgtaacc
      541 ccctcgtcag caccgtgact tcgccgctcg tcgatacgct gcagggcgtt caagacggca
      601 cgagtctcgc ctcgctgccg ctgatctacg agacgaacta ccccgccatg atcggcgcgg
      661 acaagctgca ctgccgcggc atcacgggcc gcggggtcac gatcgccgtg ctcgacacgg
      721 ggctctggcg cgacaccttt cagttctacg ggaaccgcat cctcgcgacg gtcgacgtca
      781 cgtacggcgg ccgcggtccc gtcacgggcg acccctacgg tcacggcact cacgtgacgt
      841 cgatcgcggc gagcggcgcg caggcgctct ccggcggcta tcagggcatc gcgccgcagg
      901 cgaacgtcgt cgtcgtgcgc gcgttcaacg gcacgggcgc cggccgctac gtcgacgtca
      961 tcgcgggctt gaactggatc gtccagaacc gcgcgcggca caacatccgc gtgctgaacc
     1021 tgtcattcgg cgccgcgccg cagtcgttct actgggacga tccgctgaac caggccgtga
     1081 tggccgcgtg gcgagccggg atcgtcgtcg tcgcgtcggc cggtaacgac ggccccgacc
     1141 cgatgaccgt caacgtgccg ggcaacgtgc cgtacgtgat cacggtcggc gcgctgaccg
     1201 acagcaacac gccgtacgac tacacggacg acaagctcgc gtcattctct tcgacgggac
     1261 cgacgttcga gggcttcgtc aagcccgagg tcgtctcgcc gggcggccac atcaaggcct
     1321 cgatgtcgag cagcagcttc ctcgcgtctc tcgacccgaa gtctgtgcaa gcgacgcagc
     1381 agctcttcac gatgtcgggc acgtcgcaag cggcggccgt gacctcgggc gtcgtcgcgc
     1441 tgatgctgca agacgagccg tggctgacgc ccgacacggt caaatgccgc ctcatggtgg
     1501 ccgcgcggcc cgccgtgaac acgaacggca cgctcgcgta cagcgtgttc cagcaaggcg
     1561 ccggtctcat caacgcgctc gatgccgtca acagcaccgc gtcgagctgc gcgaaccgcg
     1621 ggctcgacat cgacgacgac ctcgccgacc ggcgccactt cggcggcccg gccaacgagg
     1681 acggcaacgg cggctactac atcatggata tggagggccg gcgctggggc gactccctcg
     1741 actccgacgg ctacacgtgg tcgcgcggct atccgtgggg cgaaggctac tcctggagcc
     1801 agggctacac ctggagccga ggctacacgt ggagccgcgg ctacacctgg agccgcagcc
     1861 tgtcgtggtg gaacgcgccg gccacttaca ttcccatgag caagccggct tcgatcgcga
     1921 cctgggtgcc gaacgagtaa tagcggtgtg acgcaagtcc ggtcgggata cggtgcatgg
     1981 acgcccggat tcccgacgcg gatcaaagcc ccaagacgcg cgcgcgcgga gacttctcgc
     2041 gtgagatcga atgaccggcc gtgcgccgca tggcgctcgt gcctagctca aggattgttc
     2101 gccatgatgg gggcgtccgc cgcggcggcc gacgaccccg cgctcgttct cgaccatctc
     2161 acgaccgagg acggcctgcc gcaggcgacc gtcatgacga cgctgcaaga ctcgcagggc
     2221 ttcgtctggc tcggcaccga ggacggtctc gtgcgctacg acggcggcga gcttcatcgt
     2281 tatgcgcgct cgcgcacgga accggattcg ctgccgggca actacgtctg gcaagtcgtc
     2341 gaagacgcgg acacgaatct ctggatagcg ctcgacgggg ccggcgtcgc gaaatggaat
     2401 cgccgcaccg accgtttcac gagttaccgc cacgacgcga gccgcgcggc ctcgctcgcg
     2461 agcgatcgcg tccggaccgt gctcgtcgac ggcagcggca gcgtatgggt cggcacttac
     2521 gatgccggcg tcgacgtgct cgagcccaag tcggggaacg tcgagcatct gcgtcacgac
     2581 cccgacgact tgggctcgct cgcgagcgac cgcgtttcga cgctcgcgct cgatcgcgcg
     2641 ggcgacgtgt ggatcggcac ggaccgcggg ctcaatcact ggcgtgccga gacgcgcacg
     2701 atgacgcggg tcggaacgcc gtccggcgat gcgcgatcgt tgcacgacgt cgagatctcg
     2761 cgcgtcctcg aggatcagag c
//
LOCUS       DASSNN010000497         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_590459, whole genome shotgun sequence.
ACCESSION   DASSNN010000497 DASSNN010000000
VERSION     DASSNN010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590459"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="VFL84_09200"
     CDS             <1..1311
                     /locus_tag="VFL84_09200"
                     /inference="COORDINATES: protein motif:HMM:NF039990.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HET7608839.1"
                     /translation="PLFAAITLGALACTPLAHAETIDELKQAIDALTRQVAELERQQA
                     AAARERASDATKAVTAGRPKGSFKLPGSDTSVTLGGYVKLDAVFSNPSAGVGNVGDLQ
                     LSPSSIAVGPDAGDHEHNQVKFGARETRLFAKTNTPTPLGDLDTHVEIDFYGADGNES
                     VSNSHGLRLRHAYFSLGRFLAGQTWTNFMNPAALPETLDFGGPAGQIFDRQAQVRWTD
                     TVALPGIATPARWSVALENPETVATVPGGASFRADDDHVPDVTGQVALDTTFGRFSVH
                     GLVRQVSVDSGATPAANGRRIGGGISVAGIVPAIGKDDLRFTASAGNAIGRYANGFFP
                     DAVVAPRGDLALPTQWTVFAGYRHYWTDRLRSSLVLSMAHENNPAAAPANTNRSARSA
                     HVNLIWSPVDHADVGVEYLYADRETEDGQKGHLDRLQASAKYAF"
     gene            1545..>2152
                     /locus_tag="VFL84_09205"
     CDS             1545..>2152
                     /locus_tag="VFL84_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608840.1"
                     /translation="MDVSALRRALFVSGVAALGIASTAHAALFRSYLRSDGGGTACTL
                     QAPCRLLDAALAAVQDGGEIWMLDSANFNTSTVVITKSVKIFAIPGALGSVVGLNGDA
                     IYIDTSGDVTLRNLQILNFGGGLNGISVVNASAVHIEKTSIDGFSTANSSCIHVDSAN
                     TVRLYVDDSFLRHCTVGVSVLGNNAANRTSAVLDNTRIERGF"
BASE COUNT          368 a          721 c          710 g          353 t
ORIGIN      
        1 ccgctcttcg ccgcgattac gctcggtgcg ctcgcctgca cgccactcgc gcatgccgaa
       61 acgatcgacg aattgaagca ggcgatcgat gcgctgacga ggcaggtcgc cgagctcgag
      121 cgccagcagg ccgcggccgc gcgcgaacgc gcgagcgacg caacgaaagc cgtcaccgcg
      181 ggccgcccga aaggctcgtt caagctgccg gggtccgaca cgtcggtgac gctcggcggc
      241 tacgtgaagc tcgatgcggt cttcagcaac ccgagcgcag gggtcggcaa tgtcggcgac
      301 ctgcagttgt ccccgtcgtc gattgcggtg ggacccgatg ccggggatca cgagcacaac
      361 caggtgaagt tcggcgcgcg cgagacgcgg ctgttcgcga agacgaacac gccgacgccg
      421 ctcggggatc tcgacacgca cgtcgagatc gacttctacg gcgccgacgg caacgagagc
      481 gtgagcaatt cgcacgggct gcggctgcgg cacgcgtatt tctcgctcgg ccgcttcctc
      541 gccggccaga cgtggaccaa cttcatgaat cccgcggcgt tgccggaaac cctcgacttc
      601 ggcggaccgg ccgggcaaat cttcgatcgc caggcgcagg tccgttggac cgacaccgtc
      661 gcgctgccag gcatcgcgac gcctgcacgg tggtcggtcg cgctcgagaa tccggagacc
      721 gtcgcaacgg ttccgggtgg cgcgagcttt cgcgccgacg acgaccacgt gccggacgtc
      781 accgggcagg tcgcgctcga cacgacgttc ggccgctttt cggtacacgg attggtgcgg
      841 caggtcagcg tcgattcggg cgcgacgccg gccgcgaatg gccggcgcat cggaggcggc
      901 atcagcgtcg ccggcatcgt gccggcaatc ggcaaggacg atctgcgctt caccgcgagc
      961 gcgggcaacg cgatcggccg gtacgcgaac ggcttcttcc ccgatgccgt cgtcgcgccg
     1021 cgcggcgatc tcgcgctgcc gacccaatgg acggtattcg ccggctatcg ccactactgg
     1081 accgaccgcc tgcgctcgtc actcgtgctc tcgatggcgc acgagaacaa tccggccgct
     1141 gcgccggcca acacgaatcg cagtgcgcga tcggcgcacg tcaacctgat ctggagtcca
     1201 gtcgaccacg cggacgtggg ggtcgaatac ctctacgccg atcgcgagac cgaagacggg
     1261 cagaaggggc atctcgaccg gctgcaggca tcggcgaagt atgcgttctg agaatgcgcc
     1321 gggcggacgt tgacactgcc gaagcggagt gcttcactgc cgcatcgccg ctgccgccgc
     1381 agcgggcatt tgacagggga ggtcaagtgc gtcccgattc ctgatacacc cttcacgtcc
     1441 gacgaagggg cgccccgtgt caagcgcacg gggcggccat cgccgcgacg ctccgctgcg
     1501 caccacacgc cttcgttcgg tcttcgtctt caccggaggg aaagatggac gtttccgcat
     1561 tgcgtcgtgc actgttcgta tcgggcgtcg cggcgctcgg catcgcgtcg accgcgcatg
     1621 ccgcgctgtt ccgcagctat ctcaggtcgg acggcggcgg cactgcgtgc acgctgcagg
     1681 ccccgtgccg attgctcgat gccgccctgg ccgcggtgca ggacggcggc gagatctgga
     1741 tgctcgattc ggcgaatttc aatacgtcga ccgtggtcat caccaagtcg gtcaagatct
     1801 tcgcgatacc gggggcgctc ggcagcgtcg tcggtctcaa tggcgatgca atttatatcg
     1861 acacgagcgg cgacgtcacg ctgcgcaatc tgcagatcct gaacttcggc gggggtctga
     1921 acggaatcag cgtcgtcaac gcaagtgctg tccatatcga aaagacgtcg atcgacggct
     1981 tcagcaccgc caacagctcc tgcattcacg tcgactcggc gaatacggtt cggctgtacg
     2041 tcgatgattc gttccttcgt cactgcaccg tcggcgtgtc cgtcctcggc aacaacgcgg
     2101 cgaaccgcac gtcggcggtt ctcgacaaca cgcggatcga gcgcggcttc aa
//
LOCUS       DASSNN010000498         3259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_216215, whole genome shotgun sequence.
ACCESSION   DASSNN010000498 DASSNN010000000
VERSION     DASSNN010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216215"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFL84_09210"
     CDS             complement(<1..394)
                     /locus_tag="VFL84_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HET7608841.1"
                     /translation="MSRPLADYTSTELRSIDELDAAIVSLAKQMNIDCYRMLVLVREF
                     DDRFGWMKWSFKNCAEWLAWRCSLKPSTAREKVRTAHALRRLPAIAKAFADGRLSYSK
                     VRALTRVADGEQQDKLLRYALEATVPDVE"
     gene            650..1882
                     /locus_tag="VFL84_09215"
     CDS             650..1882
                     /locus_tag="VFL84_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007106334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HET7608842.1"
                     /translation="MYTRLSVMMFLEFFIWGAWFVTMGTYLTGPLSFSDGENALAYAT
                     QSWGAIIAPFVIGLIADKYVNAERLLGVIHLVGAVLLYLLARTSAFATFYPYLLLYMI
                     LYMPTLALVNAISFRQLDDPARRFGLIRFWGTFGWVVAGLLISFGFDWDSQADQLRNT
                     FTMAAVASLALGVFSFTLPATPPKKAAVGGGLREALGLDALALLRDRNFLVFFVASIL
                     ICIPLAFYYQHANQFLTEIRVANAAGIQTIGQVSELGFMLLVPFFLKRFGMKTTLLIG
                     MLAWAARYVLFAYGNAGELAFMLILGIALHGVCYDFFFVSGQVFTDSKAGPRYKSAAQ
                     GLITLATYGVGMLIGFWVAGQLTAAFAVDGGHDWRSIWIYPAAFAAGVAVLFALLFRN
                     EAVAYEPKPTATPARDHG"
     gene            1875..2711
                     /locus_tag="VFL84_09220"
     CDS             1875..2711
                     /locus_tag="VFL84_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET7608843.1"
                     /translation="MASYRYNTVDVFTAKPLYGNGLAVVHDAKGLSDDLMQRFTRWTN
                     LSETTFLLPTTDPAADYNVRIFFPGGELPFAGHPTLGTCHAWLAAGGKPKNRDEIVQQ
                     CGVGLVRVRRDGTRLAFAAPPLKRTGPADDATLDSIARSLAIKRSAIRDSQWVVNGPN
                     WVAVMLGSREELLALKPDMTNLEPHDIGVVAPMPKGSDAAFEMRAFVGGGGPFEDPVT
                     GSLQAGLAQWLIGAGKAPDRYVAVQGTALGRDGRVYVEKVGQDIWIGGDSVALVEGKV
                     TL"
     gene            2747..>3259
                     /locus_tag="VFL84_09225"
     CDS             2747..>3259
                     /locus_tag="VFL84_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608844.1"
                     /translation="MARGAALAAASTLAPRDLLAQVARGATCGGPPAGVQLFTVREAL
                     GRDVRGALRTLREIGIVEAELFGLNGPESATLFGLPARDLKRALDDSGIRVPISHVGG
                     NLENSAAIADIAHTLGLSALCVALPSEFSGSGGMVPAKSRAQLDALAEKLNRVGREYR
                     ERGLTFGYHNH"
BASE COUNT          524 a         1110 c         1040 g          585 t
ORIGIN      
        1 cctcgacgtc gggaaccgtc gcctcgagcg cgtaccgaag cagcttgtct tgctgctcac
       61 cgtcggcaac ccgcgtcagc gcccgcacct tcgagtacga cagccggccg tcggcaaacg
      121 ccttcgcgat cgccggcaac cggcgcagcg cgtgcgccgt gcgcaccttc tcgcgcgccg
      181 tcgacggctt caagctgcag cgccacgcga gccactcggc gcaattcttg aacgaccact
      241 tcatccagcc gaagcgatcg tcgaactcgc gcaccagcac gagcatccga tagcaatcga
      301 tgttcatctg cttcgcgagg cttacgatcg ccgcatcgag ctcgtcgatg ctgcgaagct
      361 cggtcgaggt gtaatcggcc aagggacggg acatcacggc gctcatcgga agccccccaa
      421 aaggtgtgat tggaatgggc tggcattata tacagccaaa gagccgatta gccaataata
      481 atcagctatt tacagtgctt tcagccgtgg acaaccgccg ctcgaatcgc tggtgcgtgc
      541 cggccggcac cctatactcg tcttgctaca cgacgtagcg acttaacgcg aagaaccagg
      601 cgcattgacc tggatcgaca ataacgagga cggccccttg agcatcggaa tgtacacgcg
      661 cttgagcgtc atgatgttcc tcgagttctt catctgggga gcctggttcg tgacgatggg
      721 cacctatctc acgggccctt tgagcttcag cgacggcgag aacgcgctcg cgtacgcgac
      781 gcaatcgtgg ggcgcgatca tcgcgccgtt cgtgatcggg ctcatcgcgg acaagtacgt
      841 gaacgccgag cggctgctcg gcgtgatcca cctcgtcggc gcggtgctgc tgtatctgct
      901 cgcgcggacg tccgcgttcg cgaccttcta cccttatcta ttgctgtaca tgatcctgta
      961 catgccgacg ctcgcgctgg tcaacgcgat ctcgttccgc cagctcgacg atccggcccg
     1021 ccgcttcggc ctcattcgct tctggggcac gttcggctgg gtcgtggccg ggctcctgat
     1081 cagcttcggc ttcgattggg actcgcaagc cgatcagctt cgcaatacgt tcacgatggc
     1141 ggccgtcgcg tcgctcgcgc tcggcgtctt cagcttcacg cttccggcca cgccgccgaa
     1201 gaaggccgcc gtcggcggag gcctgcgcga agcgctgggt ctcgatgcgc tcgcattgct
     1261 gcgcgaccgg aatttcctgg tcttcttcgt ggcgtcgatc ctgatttgca tcccgctcgc
     1321 gttctactac cagcacgcga atcaattcct gaccgagatc cgcgtcgcga acgccgcggg
     1381 catccagacg atcggccaag tgtcggagct cggcttcatg ctgctcgtgc cgttcttctt
     1441 gaagcgcttc ggcatgaaga cgacgctctt gatcggcatg ctcgcgtggg cggcgcgcta
     1501 cgtgctgttc gcctacggca acgccggcga gctcgcgttc atgctgatcc tcggcatcgc
     1561 gctgcacggc gtctgctacg acttcttctt cgtctcgggc caagtgttca cggactcgaa
     1621 agccgggccg cgctacaaga gcgccgcgca agggctcatc acgctcgcca cttacggcgt
     1681 cggcatgttg atcggctttt gggtcgcggg gcagctcacg gccgcgttcg cggtcgacgg
     1741 cggccacgac tggcgctcga tttggatcta cccggccgca ttcgccgcag gcgtagcggt
     1801 gttgttcgcg ctattgttcc gcaacgaagc cgtcgcgtac gaacccaagc cgacggcgac
     1861 accggcacgc gaccatggct agctatcgct acaacaccgt cgacgttttc actgcgaagc
     1921 cgctttacgg caacggcctc gcggtcgtcc acgacgccaa gggtttgagc gacgacttga
     1981 tgcagcggtt cacgcgctgg acgaacctga gcgagacgac cttcctgctg ccgacgacgg
     2041 acccggcggc ggactacaac gtgcgcatct ttttcccggg cggcgagttg ccgttcgcgg
     2101 gccacccgac gctcggcacc tgccacgcgt ggctcgccgc cggcggcaag ccgaagaacc
     2161 gcgacgagat cgtgcagcag tgcggcgtcg gcctcgtgcg cgtgcgccgc gacggcacgc
     2221 gcctcgcatt cgcggcgccg ccgctgaagc gcaccggccc ggccgacgat gcgacgctcg
     2281 attcgatcgc gcgcagctta gctataaagc gcagcgccat ccgcgactcg cagtgggtcg
     2341 tgaacggtcc gaactgggtc gccgtcatgc tcggctcgcg cgaggagctg ctcgcgctga
     2401 agcccgacat gacgaacctc gagccgcacg acatcggcgt cgtcgcaccg atgccgaaag
     2461 gcagcgacgc ggctttcgaa atgcgcgcat tcgtcggcgg cggcgggccg ttcgaggacc
     2521 ccgtgacggg cagcctccaa gccggcctcg cgcagtggct gatcggcgcc ggcaaggcgc
     2581 ccgaccggta cgtcgcggtg cagggcaccg cgctcggtcg ggacggccgc gtctacgtcg
     2641 agaaggttgg gcaagacatc tggatcggcg gcgactcggt ggcgctcgtc gaaggcaaag
     2701 tcacgcttta acgttcagag gtatctcgat gaaccggcga gattttatgg cccgcggcgc
     2761 ggcgctcgct gcggcctcca cgctcgcgcc gcgcgacctc ctcgcgcagg tcgcgcgcgg
     2821 cgctacctgc ggcggtccgc ccgccggcgt gcagctgttc acggtgcgcg aagcgctcgg
     2881 ccgcgacgtg cgcggcgcgc ttcgcacttt gcgcgagatc ggcatcgtcg aagccgagct
     2941 gttcggcttg aacggccccg agagcgcgac gctgttcggg ctgccggcgc gcgacttgaa
     3001 gcgcgcgctc gacgacagcg gcatccgtgt gccgatctcg cacgtcggcg gcaacctcga
     3061 gaattccgcc gcgatcgccg acatcgcgca cacgctcggt ctctcggcgc tctgcgtcgc
     3121 gttgccgagc gagttcagcg gcagcggcgg catggtgccg gccaagagcc gcgcgcagct
     3181 cgacgcgctc gccgagaaat tgaatcgcgt cggccgcgag taccgcgagc gcgggctcac
     3241 cttcggctat cacaaccat
//
LOCUS       DASSNN010000499         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1834255, whole genome shotgun sequence.
ACCESSION   DASSNN010000499 DASSNN010000000
VERSION     DASSNN010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834255"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VFL84_09230"
     CDS             <1..575
                     /locus_tag="VFL84_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608845.1"
                     /translation="GEPLVTATELDEDVSGIHYSLAQYYESYSPDVEPGAAGLDGDLR
                     AIFDDLKDTDEGSHETRPAAELIRTHERQLMASVFRWTGHFPEKTRSLVRDLAKRAEA
                     LKQVYAREAEDEAVVAVTTLVTSLAMNFVHRGAYFPEAAPASQENASPPPQPSAEEAR
                     SDGQEDASAPLPPAESLPASEREDLKRVSR"
     gene            complement(565..1596)
                     /locus_tag="VFL84_09235"
     CDS             complement(565..1596)
                     /locus_tag="VFL84_09235"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET7608846.1"
                     /translation="MEITVLAYLEPDEKEPDIVVRQVAAGLAERGHKTSILTIRHDLN
                     ELIEGLKQRKPQLVFNLVESFGDDIMGGVIGVAGVLDLLQIPYTGGGPGEIFLQEDKA
                     LTKKLLAFEGIRYPDFATFPADANFETGGNLRMPLFVKPLRMDASIGIDEKSLVRNTQ
                     QMMERVLYIQRTFGDAALAEEYIEGREFYVGVMGNGELIAFPPIEMDFSGLKEGSLKV
                     MDNSAKFDENSDRFHGTKAVLASIEPELKAKLQKASIDAYRALRVCDYGRVDLRLTET
                     GEVYVIEVNASCYLEEQSEFATAAAALGIPYPELVERIAELAVERWKHRHRAQKRRKK
                     ARTLAAATG"
     gene            1810..>2376
                     /locus_tag="VFL84_09240"
     CDS             1810..>2376
                     /locus_tag="VFL84_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7608847.1"
                     /translation="MNDRSKCAALLVAAAVLSGCGGADETAAPPPDFSRAVTVERLRN
                     AASEPQNWLTHGGTYLEQRHSPLTQINKETLARLAPAWFFEFDTNRGQEATPLVVDGV
                     LYTTTAWSKAYALNAATGELIWSYDPKVPGAAGAKACCDVNSRGPALYMDKVIIATLD
                     GRLIALDRATGAPLWSTVTVDQSKMYTIT"
BASE COUNT          429 a          775 c          771 g          401 t
ORIGIN      
        1 tcggcgagcc gctcgtaacc gcgacggagc tcgacgagga cgtcagcggc atccattact
       61 cgctcgctca gtactacgaa agctactccc ccgacgtcga gcccggcgcc gcgggcttgg
      121 acggcgacct gcgcgcaatc ttcgacgacc tcaaagacac cgacgagggc tcgcacgaga
      181 cgcgccccgc ggcggagttg attcgaacgc acgagcggca gctcatggcg agtgtcttcc
      241 gctggaccgg ccactttccc gagaagacgc gcagcctcgt ccgcgatctc gcgaagcgcg
      301 cggaagcgct gaagcaagtc tatgcgcgcg aggccgagga cgaagccgtc gtcgccgtga
      361 cgacgctcgt gacgtcgctc gcgatgaact tcgtgcacag aggcgcgtat tttcccgagg
      421 ccgcgccggc gagtcaggag aacgcgagcc cgccgccgca gccgagcgcc gaagaagcgc
      481 gctccgacgg gcaagaggat gcgagtgcgc cgctgccgcc ggccgagtcg ctgccggcga
      541 gcgagcgaga ggacctgaag cgagtcagcc ggtagccgcg gccaaggtgc gggctttctt
      601 gcggcgcttc tgcgcgcgat ggcgatgctt ccagcgctcg acggccagct cggcgatacg
      661 ctcgacgagc tcgggatagg ggatgccgag cgccgccgcc gccgtcgcga actcgctctg
      721 ctcctcgaga tagcagctcg cattgacctc gatgacgtag acctcgcccg tctccgtaag
      781 acgcaaatcg acgcggccgt aatcgcagac gcgcagcgcg cgataggcgt cgatcgaggc
      841 cttctggagc ttggccttga gctcgggctc gatcgacgcg aggacggcct tcgtgccgtg
      901 gaaacggtcg ctgttctcgt cgaacttcgc gctgttgtcc atgaccttga ggctgccctc
      961 cttcaagccc gagaaatcca tctcgatcgg cgggaacgcg atgagctcgc cgttgcccat
     1021 gacgccgacg tagaactcgc ggccctcgat gtactcctcg gcgagcgcgg cgtcgccgaa
     1081 cgtgcgctgg atatagagga ctcgctccat catctgctgc gtgttgcgca cgagcgactt
     1141 ctcgtcgatg cctatcgaag cgtccatgcg cagcggcttc acgaacagcg gcatgcgcag
     1201 attgccgccc gtttcgaagt tcgcgtccgc cggaaaggtc gcgaaatccg ggtagcgaat
     1261 gccctcgaaa gccaagagct ttttcgtgag cgccttgtct tcctgcagga agatctcgcc
     1321 ggggccgccg ccggtgtatg gaatctggag caaatcgagc acgcctgcga cgccgatgac
     1381 gccgcccatg atgtcgtccc cgaacgactc gaccaagttg aaaacgagct gcggcttgcg
     1441 ctgcttcagg ccttcgatga gctcgttgag gtcgtggcgg atcgtcaaga tggaagtctt
     1501 gtggccgcgt tcggcgagac cggcggcgac ttgccgcacc acgatgtcgg gctccttctc
     1561 gtccggctct aggtaagcga ggaccgtaat ttccatcggt cgtcagtgcg cggctactgc
     1621 catcacgccg aactgtaacg acttaagtcc cgagcgacaa cgatcctgcc tcgcgacgtg
     1681 catgcggtca caccgaatgg aagcgaattg ccgacgacag cacacttcac tgccgcctcg
     1741 tccgctctgc catctcgagg cgcttgcggc tatcgttcgc ctatacggtt gcgatgccgg
     1801 cgagttcaaa tgaacgacag atcgaaatgc gccgctctgc tcgtcgcagc ggcggtgttg
     1861 agcggatgcg gcggcgccga cgagactgcg gcgccgccgc ccgatttttc gcgtgccgtc
     1921 accgtagagc gcctgcggaa cgcggcaagc gagccgcaga actggctcac gcacggcggc
     1981 acctacctcg agcagcgtca ttcgccgttg acgcagatca acaaggagac gctggcaagg
     2041 ctcgcgcccg cttggttttt cgaattcgat acgaatcgcg gtcaggaagc cacgccgctc
     2101 gtcgtcgatg gcgtgctcta tacgacgacc gcctggagca aggcgtatgc gctgaacgcg
     2161 gcgacgggcg agctgatctg gagctacgac ccgaaggtgc ccggggccgc cggcgcgaaa
     2221 gcgtgctgtg acgtgaactc gcgcggtcca gcgctgtaca tggacaaggt catcatcgcg
     2281 acgctggacg ggcggctcat cgccctcgac cgcgcgacgg gcgcgccgct ctggagcacg
     2341 gtcaccgtcg atcaatcgaa gatgtacacg atcacg
//
LOCUS       DASSNN010000500         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1217834, whole genome shotgun sequence.
ACCESSION   DASSNN010000500 DASSNN010000000
VERSION     DASSNN010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217834"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1388)
                     /locus_tag="VFL84_09245"
     CDS             complement(24..1388)
                     /locus_tag="VFL84_09245"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7608848.1"
                     /translation="MNTAVARKRSIAPAAPAIGPATTVEVSTPTEIQQVLIDARRYPS
                     PVRPVGSASSMTRCITASGGTQLDLSKMNRVLKIEGDRVTVQPGISLTELADVLADEG
                     LELISGFDLAGRTVGGAVSAAGLEASMAGDVGQFASHVVQLKVLSPNGKKFVVTEKTK
                     SLLALMRLSYGLLGVVYEVTLRVRPVQGFAVQTAKVSFKDFGQLGTKLLGATAGVKLY
                     LLPFRDRIYLELRRPAAEADPGRKFAWRFKDWAVYSALPEAARSLGMAMPIRQIRYPL
                     IDSLSEVAQRLVNNSLVRSGSNSVEQSGRYRLLGSKSRFSYTTWAFPASEFANTALAY
                     KLFCKEHYARTGFRCDMPTVGFRLNRDRSALLSPSFDSPMFTLSPLSTQDEGWDDFVL
                     DFSDFAAKHRGVPFFNQTRNAAPEVVTQRFGSRLAFFNKVRRELDPLDRLVNQFFQSY
                     LPQA"
     regulatory      complement(1462..1554)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1631..>2592)
                     /locus_tag="VFL84_09250"
     CDS             complement(1631..>2592)
                     /locus_tag="VFL84_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608849.1"
                     /translation="GFRYEPQAAAAPFESFERTDIELSEPFDETRTHEIFHLSFPSSG
                     RNGHPENLVEGQYFRSKEPGRKSLVVVMPIWGTSDYPPAKVSAGYAKRAGNDTHVIWI
                     YGTAPLFPWDELSSVPTEEGFRRVARESAERYRAAVVDMRHLVDWATTRPEIDPDRIA
                     FVGFSMSALVTATLLANEPRVAGGVLMMGAARYADIFATCQNRAGDVRNHAMRAFGWT
                     VDEYREFFKGLFDPADPVRFEGRYDPNKILMIDAMFDDCMPESSRAALWEITGHPERV
                     TFLYRHRSAFYSLTPLGLNVSRRKIYRFLDEIVGDKRERNAAD"
BASE COUNT          459 a          822 c          860 g          451 t
ORIGIN      
        1 tcccacctgc aactgagccg ccgtcaggcc tgcggcagat acgactgaaa gaactgattg
       61 acgagcctgt cgagcggatc gagctcgcgg cggaccttgt tgaagaacgc gagccgcgag
      121 ccgaagcgct gcgtgacaac ctcgggcgcc gcgttccgtg tttgattgaa gaacggcacg
      181 ccgcgatgct tcgcggcgaa gtcggagaag tcgagcacga aatcgtccca tccttcgtcc
      241 tgcgtcgaga gcgggctcaa cgtgaacatc gggctatcga acgacggaga gagcagggcg
      301 ctgcgatcgc ggttcagccg gaatccaacc gtcggcatgt cgcagcggaa acccgtgcgc
      361 gcgtaatgct ctttgcagaa gagcttgtac gcgagcgccg tgttcgcgaa ctcgctcgcc
      421 ggaaaggccc aggtcgtata ggagaagcgg ctcttgctgc cgagcagccg atagcgcccg
      481 ctttgctcga cggaattgct gccgctgcgc acgagcgagt tgttcacgag cctctgcgca
      541 acctcgctca agctgtcgat cagcggatag cggatttggc gaatcggcat cgccatgccg
      601 agcgagcgcg ccgcctcggg tagcgcggag tacacggccc agtccttgaa ccgccacgcg
      661 aacttgcgcc cgggatcggc ttcggctgcc ggccggcgta gctcgaggta gatgcgatcg
      721 cggaacggca ggagatagag cttcacgccg gccgtcgcgc cgagcagctt ggtgccgagc
      781 tggccgaaat ccttgaacga gaccttcgcc gtttgtacgg caaatccttg cacgggacgc
      841 acgcgcagcg tgacctcata gacgacgccg aggagcccgt agctgagccg catcagcgcg
      901 agcaggctct tcgtcttctc ggtaacgaca aatttcttgc cgttcgggct caggactttg
      961 agctgcacga cgtgcgacgc gaactggccg acgtcgccgg ccatcgatgc ctcgagcccc
     1021 gcggccgaga cggcgccgcc gaccgtgcgg ccggcaagat cgaatccgct gatgagctcg
     1081 agcccttcgt cggcgagaac gtccgcgagc tcggtcagag agatgccggg ctgcaccgtg
     1141 acgcgatcgc cctcgatctt gaggacgcga ttcatcttcg acaggtcgag ctgggtgccg
     1201 ccgctcgcgg tgatgcagcg cgtcatcgag ctcgcggagc cgacgggccg caccgggctc
     1261 ggatagcgcc tggcgtcgat caatacttgc tgaatctcgg tcggcgtgga gacttcgacg
     1321 gttgtggccg gacctattgc cggtgcagct ggagcgatgc tgcgtttgcg cgcgacggcc
     1381 gtattcatct gcggacggac tccctcaata tccgtgccgc cggtctgacg aatgagagtg
     1441 ccgcgagcgc gtgctcgagt agccggcaac cccgctatcg tagggcccgc tcggtggccc
     1501 gttagaccgg aagctttgtg tccccggctt tcaccgggtt tacctttgat cggcgacgcc
     1561 gaatatcgcc gagttcacct gtctcttgca agcgaccgcg cacgtggcgc cagaatgcga
     1621 caggtttatg tcaatcagcg gcattccgct cgcgcttgtc gccgacgatc tcgtcgagaa
     1681 agcgatagat cttgcggcgc gaaacgttga gaccgagcgg cgtcagcgaa tagaacgcgc
     1741 tgcggtggcg atagaggaac gtgacgcgct cgggatgacc ggtgatctcc cacagcgccg
     1801 cgcgcgacga ctcgggcatg caatcgtcga acatcgcgtc gatcatcaag attttgttcg
     1861 ggtcgtagcg tccctcgaag cgcacggggt cggcgggatc gaacagtccc ttgaagaact
     1921 ctcgatactc gtcgacggtc cagccgaaag cgcgcatggc atggttgcgg acgtcgccgg
     1981 cgcggttctg acaagtcgcg aatatgtccg cgtagcgagc cgcgcccatc atcagcacgc
     2041 cgcccgcgac tcgcggctcg ttcgcgagca gcgtcgccgt cacgagcgcg ctcatgctga
     2101 agccgacgaa cgcgatgcgg tcggggtcga tctcggggcg cgtcgtcgcc caatcgacga
     2161 ggtggcgcat gtcgacgacg gccgcgcggt agcgctcggc gctctcgcgc gccacgcgcc
     2221 gaaagccttc ctcggtcggc acggacgaga gctcgtccca tggaaacagc ggcgccgtgc
     2281 cgtaaatcca gatgacgtgc gtgtcgttgc ccgctcgctt cgcatagccg gccgacacct
     2341 tcgcaggcgg gtagtcgctc gtcccccaga tcggcatcac gacgacgagg ctcttccggc
     2401 ccggctcctt cgaacgaaag tactgacctt cgacgaggtt ttcgggatgc ccgttgcggc
     2461 cgctcgaggg gaacgagagg tggaatatct cgtgcgtgcg cgtctcgtcg aacggctcgg
     2521 agagctcgat gtcggtgcgc tcgaacgact cgaacggcgc cgcggccgct tgcggctcgt
     2581 aacggaagcc cg
//
LOCUS       DASSNN010000501         3073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1218062, whole genome shotgun sequence.
ACCESSION   DASSNN010000501 DASSNN010000000
VERSION     DASSNN010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218062"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="VFL84_09255"
     CDS             complement(<1..134)
                     /locus_tag="VFL84_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7608850.1"
                     /translation="MEIALSNITHRFGDLTALEGVSLHAGEGEIVAIIGPSGCGKSTL
                     L"
     gene            complement(138..1151)
                     /locus_tag="VFL84_09260"
     CDS             complement(138..1151)
                     /locus_tag="VFL84_09260"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7608851.1"
                     /translation="MLLIVAATVSVRAVAAAELTPMKIGVLRIASQAPFFMGVERGYF
                     AEAGIDAELVFFDAAVPATLAIASRDVDIAVIGFTPAFFNLAYFGGMVVIAGHSRELP
                     GFPNTGVFASNKAWDAGVRTMADLKGRVIGLTTIGSTNHYAVARIGEKYGFDVNENHL
                     VPAQGLGNLAAMLMGNQVDVASGAGTAFMGLIVKGQAHVIGWAGDEISWQSTGLASHP
                     RVIAEKRAALQAIIDTYERGASEYSAVLLDKNGDGTYKDPAKAEELLALISRWTSVPP
                     NAIAQSLAYIDSRLDVGSIYEMVDWYRNQRMIEKDTDPVKFIDLSFVEGHINIPAAMK
                     AAQ"
     gene            complement(1618..1989)
                     /locus_tag="VFL84_09265"
     CDS             complement(1618..1989)
                     /locus_tag="VFL84_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7608852.1"
                     /translation="MEFSQPKGIYQQIADQMRDRILAGEWHEGERIPSVRELAGGIGV
                     NPNTVTKSYQALLDREIIENQRGLGYFVTADAKKRILAQMREELVRDELPKIFRTMRM
                     LGMQLSDLEPYFARFKEEQAP"
     gene            2192..>3073
                     /locus_tag="VFL84_09270"
     CDS             2192..>3073
                     /locus_tag="VFL84_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608853.1"
                     /translation="MSSSQGFRESNLFGAPIRDLGLKIAGTRLEPVIDEFRKELAQRG
                     LRVVPRFHLSTEWGVPFGTVVIGIPFYLAHPDLTALHGEQVGHIEGFNHADILRYLRH
                     EMGHVVNYAYKLYDREAWVKLFGSITQPYREEYRPQPFSRRFVRHLPGWYAQKHPDED
                     WSETFAVWMTPDRDWRADYAQLPTALAKLDYCARTLAELGEPLVTATELDEDVSGIHY
                     SLAQYYESYAPDVEPGAAGLDGDLRAIFDDLKDTDEGSHETRPAAELIRRHERQLMAN
                     VFRWTGHFPEKTRSLVRD"
BASE COUNT          546 a         1006 c          973 g          548 t
ORIGIN      
        1 agcagcgtcg acttgccgca ccccgacggg ccgatgatcg cgacgatctc gccctcgccc
       61 gcatgcagcg atacgccctc gagcgcggtg agatcgccga agcggtgcgt gatgttggaa
      121 agcgcgatct ccatgcgcta ctgcgccgcc ttcatggcgg caggaatgtt gatgtgaccc
      181 tcgacgaagc tcaggtcgat gaacttcacg ggatccgtat ccttctcgat cattcgttgg
      241 ttacgatacc aatcgaccat ctcgtagatg ctgccgacgt cgagcctgct gtcgatgtag
      301 gcgagggact gcgcaatagc gttcggcggc accgatgtcc agcgcgagat cagcgcgagc
      361 agctcttccg ctttggcagg gtccttgtag gttccgtcgc cgttcttgtc gagcagcacg
      421 gccgaatact cggaggcgcc gcgttcgtag gtgtcgatga tcgcttgcag cgccgcgcgt
      481 ttttcggcga tcactcgcgg gtgcgaggcg agccccgtcg actgccacga gatctcgtcg
      541 ccggcccagc cgatcacgtg cgcctggccc ttgacgatga ggcccatgaa cgccgttccc
      601 gcgccggaag caacgtcgac ctgattgccc atcagcatcg cggcgagatt gccgagcccc
      661 tgcgccggca cgaggtggtt ctcgttgacg tcgaagccgt acttctcgcc gatgcgggcg
      721 acggcgtagt ggttcgtcga gcctatcgtc gtgagcccga tgacgcggcc tttgagatcc
      781 gccatcgtcc gcacgccggc gtcccaggct ttgttcgacg cgaacacgcc cgtattcggg
      841 aagcccggca gctcgcggct gtgccccgcg atgacgacca tgccgccgaa gtacgcgaga
      901 ttgaagaacg ccggcgtgaa tccgatgacg gcgatgtcga cgtcgcgcga ggcaatcgcg
      961 agcgtggccg gcacggccgc gtcgaagaac acgagctcgg cgtcgatgcc ggcttcggcg
     1021 aaatagcccc tctcgacgcc catgaagaac ggggcctgcg aagcgatgcg cagcacgccg
     1081 atcttcatcg gcgtgagctc ggctgcggcg accgcgcgca ctgacaccgt ggcagcgacg
     1141 atcagcagca aagccgcgac tcgtgtcggc cgcgaactgc gcaaaagact cccccgtgaa
     1201 tctcgtgtcg ctgccaaaat acgcggaaac gcgtgcggaa ggaatggaat ctttcgatcg
     1261 ctgagtctgg ctggcctaaa gaaatgggtc cgagaatctc ggatcctgga ggaactccgg
     1321 atccgtaaac gcatgcaaga atgcttcgag cgcatcctta tcggacgcgc cgaggttcag
     1381 ccgcaaaggg ctgcctgtcc cgccttcgcg aagcgcggag cccaactgcg gcgcgtcctc
     1441 aataccatgg tcgtagtggt cgagttggtc agcgagccga cgatatgcac accgtcgacg
     1501 acgagcccga ggggcggcga cacggccgcc accgaaaacg tcaaccgatc gccatcgccg
     1561 tcgacgaagg cgctcccgcc gtacaccgag cattgccgca atcgcgctga ctgttcctca
     1621 cggcgcctgc tcttctttga accgcgcgaa gtacggctcg aggtccgaaa gctgcatgcc
     1681 gagcatgcgc atcgtgcgga agatcttcgg caactcgtcg cgcacgagct cctcgcgcat
     1741 ctgcgcaagg atccgcttct tcgcgtcggc ggtcacgaaa tagccgaggc cgcgctggtt
     1801 ctcgatgatt tcgcggtcga gcaacgcttg atagctcttc gtcacggtgt tcgggttcac
     1861 gccgattccg ccggcgagct cgcgcaccga cggaatacgc tcgccctcgt gccactcgcc
     1921 cgcgaggatg cgatcgcgca tctgatcggc gatctgctgg tagatgcctt tcggttggct
     1981 aaattccatg gctcagctgc gcctcgctga tgtgttggtg tgaaatagtg tcctattgac
     2041 gtatgacact aagagactca cgctttcggc gtcaagtgcg ggcccgaaac ccgcaccgaa
     2101 cctcggggtg gaacgggtcg atggcgggcc cgatcgacgg tgtcagaatg atcgaatccc
     2161 gaacccccac cctcgagctg cgttgccgcc catgtcttcg tcgcagggtt ttcgtgaatc
     2221 gaacctcttc ggtgcgccga ttcgcgatct cgggctcaag atcgcgggca cgcggctcga
     2281 gcccgtcatc gacgagttcc gcaaggagct cgcgcagcga ggcttgcgcg tcgtgccgcg
     2341 ctttcacttg tcgaccgagt ggggcgtgcc gttcggcacc gtcgtgatcg gcatcccgtt
     2401 ctatctcgcg catcccgacc tgacggcgct gcacggcgag caggtcggcc atatcgaggg
     2461 attcaaccac gcggacatcc tccgctacct gcggcacgag atgggccacg tcgtgaacta
     2521 cgcctacaag ctctacgacc gcgaggcgtg ggtgaagctg ttcggctcga tcacgcaacc
     2581 gtatcgagag gagtaccggc cgcagccatt cagccgccgg ttcgtgcgcc atctccccgg
     2641 ctggtacgcg cagaagcatc ccgacgagga ctggtcggag acgttcgccg tgtggatgac
     2701 gcccgatcgc gactggcgcg ccgactacgc gcagctgccg acggcgctcg cgaagctcga
     2761 ctattgcgcg cggacgctcg ccgagctcgg cgagccgctc gtaaccgcga cggagctcga
     2821 cgaggacgtc agcggcatcc attactcgct cgcccagtac tacgagagct atgcgcccga
     2881 cgtcgagccc ggcgcggcag gcttggacgg cgacctgcgc gcgatcttcg acgacttgaa
     2941 ggacaccgac gagggctcgc acgagacgcg ccccgcggcc gagctgattc gccggcacga
     3001 acggcagctc atggcgaacg tgttccgctg gaccggccac tttcccgaga agactcgcag
     3061 tctcgtgcgc gat
//
LOCUS       DASSNN010000502         3762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_217037, whole genome shotgun sequence.
ACCESSION   DASSNN010000502 DASSNN010000000
VERSION     DASSNN010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217037"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="VFL84_09275"
     CDS             complement(<1..1473)
                     /locus_tag="VFL84_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7608854.1"
                     /translation="MTMPVWLDVLKRDLKLTRRLLTKNLAFTFTTIVTLALGIAASTV
                     IYTVVDAVLLEPLDYNDSGDIYRVYTVDTVGLPRGTTGGPHMHPIAEEGERIQAAIYG
                     YSFEQSIVNDEGTAFAVNEFRTSDEFFEVFTEPLLMGREFEPREDWHNTILSYQTWRD
                     VFGSDPNIIGKPINVGSGPLNVIGVAAEGFEFPVGTAMWTKIFTLPEGQPLPLLNLSG
                     YVRVQPGVSAEQAQAELDVFAGRLAAWPDGRKVELVARPLLEDIVGDLKPTLFIVSGA
                     TAVLLLIACINVANLLLARGVVRASEFALREAIGARRWRLFRQLMTESVVLCAVGGVL
                     GLGLALGAIKVLQAIGPADLPRFGTLAIDTGVFLFAAACVLLVAFGVGFAPALRVSRG
                     DLSALINSGGRSAAMARGRNRLFGSLVVAEIALAVVLVIGAGLLVRSYSQLSSADPGF
                     DPRRMVTLVLNVTGRTDVRNMRFDQERQTVAYDGTGMLAVA"
     gene            complement(1522..>3762)
                     /locus_tag="VFL84_09280"
     CDS             complement(1522..>3762)
                     /locus_tag="VFL84_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7608855.1"
                     /translation="NAIQAAMYGYSFENSVVNNEGTAFAVNEFRTSQEFFKVFTEPMH
                     LGRSFEPSDNFRNTILSYQTWRDVYGSDPDIINRPINVASGPLTVIGVAAEGFEFPVG
                     TKMWTLIFTGRDAGMNSPLPLFNMPGYVRATPGVTGAQVQAELDVFAGRLSESFPWPD
                     GRKLEFVSEPLLHEVVGDLRGTLFIVSGATAVLLLIACINVANLLLARGVARVSEFAL
                     REAIGARRWRVFRQLMTESFALCSLGGILGLGIALGAIQLLKAIGPADLPRLSTVSID
                     TNVFLFAAGCVVLVALVVGFAPALRVARGDLSALLNAGGRSASLARGRNRLFGALVIA
                     EIALAVVLVIGAGLLVRSYSQLASTDPGFDPSRMLTLVLNVPGRTDIQNMRADQAQRR
                     VVYDGSGLLGVTQFYEELVRRIDALPGVASAGVTSAAPLNQGLFPIVLGAFPVIGSSI
                     PVTDQLAYVSQVSPDYFTTLGIRPLAGRLLEPTDRRGGAGVVVINEAYARAYIDGDPL
                     GRRIRLPGGARPGGLAFNQLGEQTLDDGEIVGVIPDIKQATLEADVQPAVFVPTQQWH
                     VRKMAVVVRAQNDDPATLIPAIRRELEDLDSTLPGVFDVYSDVVSAGLARHRLGALVL
                     VVFAFVSLTLAAVGTYGLMSFSVNQRVNEIAVRSAFGAERGRLLKMFVTRALQLAVIG
                     IVIGVAGAVALRRIVASQLYETSALDPWVLALVPLTMVAVTLLASYVPARRASRIDVS
                     AALREN"
BASE COUNT          688 a         1259 c         1276 g          539 t
ORIGIN      
        1 cgcgaccgcg agcatgccgg ttccgtcgta agcgacggtt tgccgctctt gatcgaacct
       61 catgttccgg acatcggtcc gacccgtcac gttcaatacc agcgtgacca tgcgccgcgg
      121 atcgaagccc ggatcggcgg atgacagctg cgagtagctg cgcacgagca ggccggcgcc
      181 gatcacgagc acgacggcca acgcgatctc ggcgaccacc aacgagccga agagacgatt
      241 gcggccgcgc gccatcgcgg ccgagcgtcc gccgctgttg atcagcgcgc tcaaatcgcc
      301 gcgggatacg cgcagcgcgg gcgcgaatcc gacgccgaat gcgacgagca acacgcaggc
      361 ggccgcgaac aggaacactc cggtatcgat cgcgagggtt ccgaagcgcg gcagatcggc
      421 gggaccgatc gcctgcaaga ctttgatggc gccgagggca aggccgaggc cgagcacgcc
      481 gcccacggcg cagagcacga cgctctcggt catgagctgg cggaacaagc gccagcggcg
      541 cgcgccgatc gcctcgcgca gcgcgaactc gctcgcgcgg accacgccgc gcgcgagcag
      601 caagttcgcg acgttgatgc aggcgatcag gagcaggacc gccgtcgctc cggaaacgat
      661 gaacagcgtc ggcttcaaat cgccgacgat gtcctcgagc aacggccgcg cgacgagctc
      721 gaccttgcgg ccgtccggcc acgcggcgag ccggcccgcg aagacgtcga gctcggcctg
      781 cgcttgctcg gcggagacgc ccggctgcac gcgcacgtag cccgacaggt tcaggagcgg
      841 cagcggttgc ccttccggca gcgtgaagat cttcgtccac atggccgtgc cgaccggaaa
      901 ctcgaagccc tccgccgcca cgccgatgac gttcagcgga ccgctgccga cattgatcgg
      961 cttgccgatg atgttgggat cggagccgaa cacgtcgcgc cacgtttgat agctcagtat
     1021 cgtgttgtgc cagtcctcgc gcggctcgaa ctcgcggccc atgagcaacg gctcggtaaa
     1081 gacctcgaag aactcgtccg aggttcggaa ctcgttgacg gcgaaggcgg tgccttcgtc
     1141 gttgacgatc gactgctcga acgaataccc gtagatcgcc gcctggatcc gctcgccttc
     1201 ctcggcgatc gggtgcatgt gcggcccgcc cgtcgtgccg cgcggcagcc cgacggtgtc
     1261 gaccgtgtag acgcggtaga tgtcgccgga gtcgttgtag tcgagaggct cgagcagcac
     1321 cgcgtcgacg acggtgtaaa tcaccgtgct tgcggcgatg ccgagcgcca atgtcacgat
     1381 cgtagtaaag gtgaacgcga ggttcttggt cagcagccgc cgcgtgagct tgagatcgcg
     1441 cttcagcacg tcgagccaga cgggcatcgt catcgtcgtg cggcctcccc cgaatccagt
     1501 tgcagacctt cagccgcgag ctcagttctc ccgcagcgcg gcgctgacgt cgatgcgcga
     1561 cgcccggcgc gccggcacgt acgacgccaa cagcgtgacc gcgaccatcg tcaacggcac
     1621 cagcgcgagc acccacggat cgagcgcgct cgtctcgtag agctggctcg cgacgatgcg
     1681 ccgcaacgcg acggcgcccg cgacgccgat cacgatgccg atgaccgcga gctgcagcgc
     1741 gcgcgtcacg aacatcttga gcagcctgcc gcgctcggcg ccgaacgccg agcgcacggc
     1801 gatctcgttg acgcgctgat tgaccgagaa cgacatcaag ccgtacgtgc cgaccgcggc
     1861 gagcgtcaac gatacgaacg cgaacacgac gagcacgagg gcaccgagcc ggtggcgggc
     1921 caggcccgcc gagacgacgt ccgagtagac gtcaaagacg cccggaagcg tcgagtccaa
     1981 atcctcgagc tcgcggcgga ttgccgggat cagtgtcgcg ggatcgtcgt tctgcgcgcg
     2041 gacgacgacc gccatcttgc gaacgtgcca ttgctgcgtc ggaacgaaca cggcgggctg
     2101 cacgtcggcc tcgagagtcg cttgcttgat gtccgggatc acgccgacga tctcgccgtc
     2161 gtcgagcgtc tgctcgccga gctggttgaa cgcgagaccg cccggacgcg cgcctcccgg
     2221 cagccttatg cgccggccga gcgggtcgcc gtcgatgtac gcgcgcgcat acgcttcgtt
     2281 gatcacgacg acgccggcgc cgccgcgccg atcggtcggc tcgagcaggc ggccggcgag
     2341 cggacgaatg ccgagcgtcg tgaagtagtc gggcgaaacc tggctgacgt aggcgagctg
     2401 atcggtgacg ggaatgctgc tgccgatgac ggggaacgca ccgaggacaa tggggaagag
     2461 tccctggttc agcggcgcgg cggaggtcac gcccgcgctg gcgacgccgg gcagcgcgtc
     2521 gatgcggcgc acgagctcct cgtagaactg cgtcacgccg agcaggccgc tgccgtcgta
     2581 aaccacgcgg cgctgtgcct ggtcggccct catgttctgg atgtccgtgc gccccggcac
     2641 gttgagcacg agcgtgagca tgcggctggg atcgaagccc ggatcggtcg acgcgagctg
     2701 cgagtagctg cgcacgagca atccggcgcc gatcacgagc acgacagcca acgcgatctc
     2761 ggcgatcacg agcgcgccga acagtcgatt gcggccccgc gccagggagg ccgagcgccc
     2821 gccggcgttg agcagcgcgc tcaaatcgcc gcgcgcgacg cgcagcgcgg gcgcaaatcc
     2881 gacgaccagt gcgacgagca cgacgcagcc cgccgcgaac aagaatacgt tcgtgtcgat
     2941 cgacaccgtg gagaggcgcg gcaagtcggc ggggccgatc gccttcagca gctggatcgc
     3001 gcccaacgcg atgccgaggc cgagaattcc gcccaagctg cacagcgcga agctttcggt
     3061 catcagctgc cgaaagacgc gccagcggcg cgcgccgatt gcctcgcgca gcgcgaactc
     3121 gctcacgcgc gcgacgccgc gcgcgagcag caaattcgcg acgttgatgc aggcgatcaa
     3181 gagaagcacg gccgtggcgc cggacacaat gaacagcgtg cctcgcaggt cgccgacgac
     3241 ttcgtgcagc aacggttccg agacgaactc gagcttccgg ccgtccggcc acgggaacga
     3301 ctcgctgagg cggcccgcga agacgtcgag ctcggcctgc acttgtgcac ccgttacgcc
     3361 gggggtcgct cgaacatagc ccggcatgtt gaagagaggg agcgggctgt tcatgccggc
     3421 gtcgcgcccc gtgaagatca aggtccacat tttcgtgccg acggggaact cgaacccctc
     3481 ggccgccacg ccgatgacgg tcagcggacc gctggcgacg ttgatgggcc ggttgatgat
     3541 gtccggatcg gacccgtaca cgtctcgcca cgtttgatag ctcaagatcg tgttgcggaa
     3601 gttgtcgctc ggctcgaacg aacggccgag atgcatgggc tccgtgaaga ccttgaagaa
     3661 ttcctgcgag gtgcggaact cgttgaccgc gaacgccgtg ccctcgttgt taacgaccga
     3721 gttttcgaac gagtagccgt acatcgcggc ctgaatcgcg tt
//
LOCUS       DASSNN010000503         8959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_828602, whole genome shotgun sequence.
ACCESSION   DASSNN010000503 DASSNN010000000
VERSION     DASSNN010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828602"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFL84_09285"
     CDS             complement(<1..678)
                     /locus_tag="VFL84_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET7608856.1"
                     /translation="MNKAKASAAFETFIVPRINRGLREAGFWVLAGLSLILLLALLSY
                     NKADPAFTVAGGDETVTNRMGPAGAWFADVAFLLFGASAYLFPILVLIAAVFLFRGEE
                     LPERRQTVWRVIGFLLVIATSSGLATLHFQSPDLRETAGGILGQGVGYGLEYALGLLG
                     STVLLLVLWLAAVQLATGVSWIAVMDRTGHVVYRGVTVLHGWLGDFRIWWEGRRAKQL
                     RDEVVTKV"
     gene            802..1962
                     /locus_tag="VFL84_09290"
     CDS             802..1962
                     /locus_tag="VFL84_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7608857.1"
                     /translation="MQLKLERLTTIGAADAAAWNALAGDSPFLRHEFLHALEQSGCVG
                     DGTTWQPCYLVARDAQGLAGALPLFIKYDSHGEFVFDWSWADAYERSGRRYYPKLVSA
                     VPFTPATGRRLLLRDAQNPDVAGQLIEAARATAHELDASSLHVLFPTEPERELLAASG
                     FLTRKGCQFHWANDGYGSFDEFLSRFSADKRKKAKRERRRVAEAGIVFEHLRGNEPTP
                     ADWDAIFEFYSRTFWRRGREPYLNREFFAAVAAAMPDSLLVVLARQHGVPIATAICFR
                     NSTTLYGRYWGSGGDFHSLHFETCYYQGIDYCIREGLARFEPGTQGEHKVARGFVPQP
                     TWSCHWLRDADFHRAVGSFLTREARHVDAYIDEVEDHVPYRRAERDDAVIDA"
     gene            1973..2674
                     /gene="aat"
                     /locus_tag="VFL84_09295"
     CDS             1973..2674
                     /gene="aat"
                     /locus_tag="VFL84_09295"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET7608858.1"
                     /translation="MRSLYWISRDRRVDAFPPASEALTEPNGLLAAGGDLSPERLLAA
                     YRKGIFPWYQEGQPILWWSPDPRAVLWPDNLKVSRSLRRSLRNKRFELRVDTAFKLVV
                     AGCAEPRSYGAGTWITDDMAEAYTRLHRLGWAHSFETWQDETLVGGLYGVAIGGAFFG
                     ESMFTRVTDASKVALVHAVEFLRARGTQLIDCQVASAHTRSLGAVDVPRAEFLSRIAD
                     LCGESAQPQMWRSAG"
     gene            2745..2963
                     /gene="infA"
                     /locus_tag="VFL84_09300"
     CDS             2745..2963
                     /gene="infA"
                     /locus_tag="VFL84_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET7608859.1"
                     /translation="MAKEEAIQMEGTVVETLPNTTFRVELENGHVVTAHISGKMRKHY
                     IRILKGDAVTVELTPYDLTKGRIVYRAR"
     gene            complement(2977..5301)
                     /gene="clpA"
                     /locus_tag="VFL84_09305"
     CDS             complement(2977..5301)
                     /gene="clpA"
                     /locus_tag="VFL84_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HET7608860.1"
                     /translation="MLSTELEYCLNDAFQRAREERHEFITVEHLLLALLDTPTVIETL
                     KACGADLARLRRELKEFIEGATPRLRGEDEEGTEVQPTLGFQRVLQRAVFHVQSSGRK
                     EVTPINVLVAIFSEKQSQAVYLLGLQDVTRLDVVNFVSHGVPKVQEASKPDTDESSGE
                     SERGAETNPLERFTTNLNAQAEAGKIDPLIGRQLEIERTVQILCRRRKNNPLFVGEAG
                     VGKTALAEGLARLIVEGKVPDILRQCTIYALDMGSLIAGTKYRGDFEKRLKGVVAGLK
                     KRPGAILFIDEIHTVIGAGAASGGVMDASNLIKPLLANGELRCIGSTTYTEFRGIFEK
                     DHALARRFQKIDVLEPSVAETVEILRGLKSRFEEHHNIAYSDEALRAAAELSAKHIND
                     RHLPDKAIDVIDEAGANCRLQPESTRPRIVDVELIQNIVAKIARIPPKNVSASDRDAL
                     RNIERDLKLVIFGQDRAIAALSSAIKMARSGLGDERRPTGSFLFSGPTGVGKTEVTRQ
                     LALTLGVELVRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAITKHPHCVLLFDE
                     IEKAHPEVFNLLLQVMDHGTLTDNNGRKADFRNVTIVMTTNAGAQEAARASVGFTLQD
                     HSSDAMEVLKKLFSPEFRNRLDAIIQFEPLDEQSIARVVDKLIVELEAQLDKNDVTIE
                     LEPAARDWVAARGYDKKMGARPMARVIQEHIKRPLAEELLFGKLAEGGHVRVEVAPDG
                     ESLDLTVEPLKRELEDLRGKGPAQRELAGHKGEE"
     gene            complement(5302..5631)
                     /gene="clpS"
                     /locus_tag="VFL84_09310"
     CDS             complement(5302..5631)
                     /gene="clpS"
                     /locus_tag="VFL84_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009022289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HET7608861.1"
                     /translation="MSHDSEKGDRVDDHGLATAEAKPRLKRPPLYRVILLNDDYTPME
                     FVVEVLQKIFSLDRTTATRIMLEVHTKGKGVCGVYTYEIAETKVAQVTGLAQQHQHPL
                     LCTMEET"
     gene            5825..6739
                     /gene="gspC"
                     /locus_tag="VFL84_09315"
     CDS             5825..6739
                     /gene="gspC"
                     /locus_tag="VFL84_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HET7608862.1"
                     /translation="MDVAKRLAEWRDRPPEQWFTTVNQYAPPGVTAVLVIAIAYQLAT
                     LTWALVPGSTPAAVAPASRPSSTATAPAADYSALTGSHLFGEAAEQPEVAAAPVIDAP
                     DTTLSLTLTGILSKNEDGSGSVLIKNSRNVEKTYWVGQDIEGADGAKLHSVYADRVLL
                     DRNGRLETLRFPKDLTASAGSPMALPPLPQTQAQPAPLREVISQNASKLTDIIRPAPH
                     VQEGQIVGFRVNPGRDRATFEALGFQPGDVVTDINGTVLDDVSQGLQIFESLGEATQA
                     NVTVLRDGVPQVLVIDTTQLQKLQENRQ"
     gene            6736..8754
                     /gene="gspD"
                     /locus_tag="VFL84_09320"
     CDS             6736..8754
                     /gene="gspD"
                     /locus_tag="VFL84_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HET7608863.1"
                     /translation="MNHSTPDARRAGAWRRTPLRHAALCAALWACFVGGSARAQPQQP
                     VLYTANYRDADIRMVTEQVQQVIGRPIIIDPRVRAQLTILSNAPMTADAFYRTYLAAL
                     EVHGFIPLESGNNIMIVPDANARFGSGEDFVTQAIILDNIGAQQLVPILRPILAQYAH
                     LAAHQASNALIIADRPQNIRRVNELVRRLDQASTADIEVIQLENASADEVVRMLSQLN
                     TAAQAAGGSPPIQVIPDPRTNSVLLSGAGAVKLQMRALIAHLDTPSAEGGDSLVRYLN
                     YADAEDLATKLQAQFGGSGGGAPATPAEGASPATSGPVTIWADTGTNALVINAPERVR
                     ADMLAIINQIDIPRLQVQVDAIIVELSEEQTAALGVTWLRADDEFIGLTNFANAAGGG
                     ILGLASQAGAETPNLAAVAEGISVGIGKLSGAGGWAALLNALRGEGETNIISTPQIVT
                     LDNEEAEIRVGQEVPFVTGQFTNTGANQGSVNPFQTIQREQIGTNLKITPQINEGSGV
                     KLDIEQETSSLAATVQGAADLVTNTRTITTSVFVNDGDVLVLGGLIDDKLLESDRRVP
                     GLGKIPGLGWLFRARTSDRNKTNLMVFIRPTILKTPEDARFQTNTKYRYIQDLQRQMA
                     ADPVMLMHEFERPQLPPLPEEQELPRPDEGPTETVVPNGAPPDGSEPR"
     gene            8735..>8959
                     /locus_tag="VFL84_09325"
     CDS             8735..>8959
                     /locus_tag="VFL84_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608864.1"
                     /translation="MAANLGSDDPVVEVPVAAPSQRNLPFAFAKRHGVLIQRLHDGAA
                     EAIYRTGVAAQSIAEVRRFAGMPVRFSEVDP"
BASE COUNT         1562 a         3024 c         2884 g         1489 t
ORIGIN      
        1 caccttcgtc acgacttcgt cgcgcagctg cttcgcgcgc cggccttccc accagatgcg
       61 gaaatcgccg agccaaccgt gcagcaccgt gacgccgcga tagacgacgt gacccgtgcg
      121 atccatgacc gcgatccacg acacgcccgt cgcgagctgg acggccgcga gccatagaac
      181 gagcagcagc accgtcgagc cgagcaagcc gagcgcatac tcgaggccgt agccgacgcc
      241 ttggccgagg ataccgccgg cggtctcgcg cagatccggt gactgaaaat gcagcgtcgc
      301 gaggcccgag ctcgtcgcga tcacgagcaa gaagccgatc acgcgccaaa ccgtctggcg
      361 gcgctcgggc aactcctcgc cgcggaacag aaagaccgcg gcgatcagca cgaggatcgg
      421 gaacaggtac gccgaggcgc cgaacagcag gaacgcgacg tcggcgaacc aggcgccggc
      481 cgggcccatg cggttcgtga cggtttcgtc gccgcccgcg accgtgaacg cgggatccgc
      541 cttgttgtag ctcaagagcg cgagcagcag gatcaggctc agccccgcga gcacccaaaa
      601 tccggcttcg cgcaatcctc tattgatgcg cgggacgatg aacgtctcga atgcagcgct
      661 ggccttggcc ttattcaagt tactcgacag ttagtcgact taatgcgctg attatactca
      721 taggctgatc ttcgcctagt cggcacgcgg cgacacgccc gacggctcga cgctcggggc
      781 aacgacgggc acactcggcg catgcagctc aagctcgaac ggttgacgac catcggcgcc
      841 gccgacgcgg cggcttggaa cgcgctcgcc ggcgacagcc cgttcttgag gcacgagttc
      901 ctgcacgcgc tcgagcagag cggctgcgtc ggcgatggca ccacgtggca accgtgctat
      961 ctcgtcgcga gagacgcgca aggcctcgcc ggcgcgctgc cactctttat taagtacgac
     1021 tcgcatggcg agttcgtctt cgactggagc tgggccgacg cttacgagcg ctcggggcgc
     1081 cgctattacc cgaagctcgt ctcggccgtg ccgttcacgc cggcgacggg ccggcggctg
     1141 ctgctgcgcg acgcgcaaaa cccggacgtc gcggggcagt tgatcgaggc ggcgcgcgcg
     1201 acggcgcacg agctcgacgc gtcgtcgctg cacgtgctgt ttccgacgga gcccgagcgc
     1261 gagttgctcg cggcgagcgg gttcttgacg cgcaaaggct gtcagttcca ctgggccaac
     1321 gacggctacg gcagcttcga cgaattcttg agtcgcttca gcgccgacaa acgcaagaag
     1381 gcgaaacgcg agcgccggcg cgtcgctgaa gccggcatcg tgttcgagca tttgcgcggc
     1441 aacgagccga caccggccga ttgggacgcg atcttcgagt tttattcgcg aacgttttgg
     1501 cgtcgcggac gcgagccgta cttgaaccgc gagttcttcg ccgcggtcgc ggccgcgatg
     1561 ccggacagcc tgctcgtcgt gctcgcgcgc cagcacggcg tgccgatcgc cacggcgatc
     1621 tgcttccgca acagcacgac gctgtacggc cggtactggg gcagcggcgg cgacttccac
     1681 agcctgcact tcgagacgtg ctattaccaa ggcatcgatt actgcatccg cgagggactc
     1741 gcgcggttcg agccgggaac gcagggcgag cacaaagtgg cgcgcggttt cgttccgcag
     1801 ccgacgtggt cgtgccattg gctgcgcgac gcggacttcc accgcgccgt cggctcgttc
     1861 ctgactcgcg aggcgcgcca cgtcgacgca tacatcgacg aagtcgaaga ccacgtgccg
     1921 tatcggcgag cggagcgcga cgatgccgtc atcgatgcgt gagcggcgcg ccatgcgttc
     1981 gctgtactgg atctcgcgcg atcgccgcgt cgacgccttt ccgccggcaa gcgaggcgct
     2041 gacggagccg aacggcttgc tcgccgccgg cggcgatctt tcgccggagc gcctgctcgc
     2101 cgcctataga aaaggaatct tcccctggta ccaggaaggg cagccgatcc tgtggtggag
     2161 ccccgatccg cgcgccgtgc tctggcccga caacttgaag gtgtcgcgca gcctgcgtcg
     2221 ttcgctaagg aataagcggt tcgagctgcg cgtcgacacg gcgttcaagc tcgtcgtcgc
     2281 cggttgcgcg gagccgcgtt cttacggcgc aggcacgtgg atcacggacg acatggccga
     2341 ggcctacacc cgactgcacc ggctcgggtg ggcgcactcg ttcgagacct ggcaagacga
     2401 gacgctcgtg ggcggcctct atggcgtcgc gatcggcggc gccttcttcg gcgagtcgat
     2461 gttcacgcgc gtgacggacg catcgaaggt tgcgctcgtt catgcggtcg agttcttgcg
     2521 cgcacgcggc acgcagctca tcgactgcca agtcgcctcg gcgcatacgc gcagcctcgg
     2581 cgccgtcgac gtgccgcgcg ccgaattcct gtcgcggatc gcggacttgt gcggcgaatc
     2641 ggcgcaaccg caaatgtggc gctcggccgg ttgaagctgg ttggcggctc tacccggctt
     2701 gtgcaacaat gcgccgcccg cggcggggct cccagctgtt gctaatggcc aaagaagaag
     2761 caattcaaat ggaaggaacg gtagtggaaa cgctaccgaa cacgaccttt cgcgtcgagc
     2821 tcgagaacgg ccacgtcgtg acggcgcaca tctccggcaa gatgcgcaag cactatatcc
     2881 gcatcttgaa aggcgacgcc gtcacggtcg agctgacgcc gtacgacctc acgaaaggcc
     2941 gaatcgtcta ccgcgcgcgt tgagccgctg cgcggcttac tcttcgccct tgtggcctgc
     3001 cagctcgcgc tgcgcggggc ctttgccgcg taagtcctcg agctcgcgct tcagcggctc
     3061 gacggtgaga tccaggctct cgccgtcggg cgcgacctcg acgcgaacgt gcccgccttc
     3121 cgcgagcttg ccgaacaaca gctcttcggc gagcggccgc ttgatgtgct cttgtatgac
     3181 gcgcgccatc ggccgagcgc ccatcttctt gtcgtagccg cgagcggcaa cccagtcgcg
     3241 cgcagcgggc tcgagctcga tcgtgacgtc gttcttgtcg agctgcgctt cgagctcgac
     3301 gatgagcttg tcgacgacgc gcgcgatcga ctgctcgtcg agcggctcga actggatgat
     3361 cgcgtcgaga cgattgcgga actccggcga gaacagcttc ttcaggacct ccatcgcatc
     3421 cgacgagtga tcctgcagcg tgaagccgac ggacgcgcgc gcggcttcct gcgcgccggc
     3481 gttcgtcgtc atgacgatcg tgacattgcg gaagtcggcc ttgcggccgt tgttgtcggt
     3541 cagcgtgccg tgatccatga cctgcaacag caagttgaac acctcgggat gcgccttctc
     3601 gatctcgtcg aacagcagca cgcagtgcgg gtgcttcgtg atcgcttcgg tcaggagacc
     3661 gccctggtcg aagccgacgt aacccggcgg cgcgccgatc aggcgcgaca ccgtgtgccg
     3721 ctccatgtac tcggacatgt cgaagcgcac gagctcgacg ccaagcgtca acgcgagctg
     3781 ccgcgtgacc tcggtcttgc cgacgcccgt cggtccggag aacaggaacg agccggtcgg
     3841 ccgccgctcg tcgcccaatc ccgagcgcgc catcttgatg gccgaggaca acgccgcgat
     3901 cgcgcggtcc tggccgaaga tcacgagctt caggtcgcgc tcgatgttgc gcagcgcgtc
     3961 gcgatccgag gccgagacgt tcttcggcgg aatgcgcgcg atcttcgcga cgatgttctg
     4021 gatcagctcg acgtcgacga tgcgcggccg cgtgctctcg ggctgcaacc ggcaattcgc
     4081 gccggcctcg tcgatgacgt cgatcgcctt gtcgggcaag tggcgatcgt tgatgtgctt
     4141 cgccgacagc tcggccgcgg cgcgcagcgc ctcgtcggag tacgcgatgt tgtgatgctc
     4201 ctcgaaccgg cttttgagcc cgcgcagtat ctcgacggtc tcggcgacgg acggctccag
     4261 cacgtcgatc ttctgaaagc gtcgcgcgag cgcgtggtcc ttctcgaaga ttccgcggaa
     4321 ctccgtgtac gtcgtcgagc cgatgcagcg cagctcgccg ttcgcgagca gcggcttgat
     4381 cagattcgag gcgtccatga cgccgcccga cgccgcgccg gcgccgatga cggtgtggat
     4441 ctcgtcgatg aacaggatcg cgccgggccg cttcttgagc cccgcgacga cgcccttcaa
     4501 gcgcttctcg aagtcgccgc ggtatttcgt gcccgcgatc agcgagccca tgtcgagcgc
     4561 gtagatcgtg cactgccgca ggatatcggg cactttgccc tcgacgatga gccgcgcgag
     4621 accctccgcg agcgcggtct tgccgacgcc ggcttcgccg acgaacagcg gattgttctt
     4681 gcgccgccgg caaagaatct gcacggtgcg ctcgatctcg agctggcggc cgatcagggg
     4741 atcgatcttg cccgcttcgg cctgcgcgtt gaggttcgtc gtgaagcgct cgagcgggtt
     4801 cgtctccgct ccgcgctcgc tttcccccga cgattcgtcc gtgtcgggct tgctggcctc
     4861 ctgcaccttc ggcacgccgt gcgagacgaa gttgacgacg tcgagccgcg tgacgtcctg
     4921 cagcccgagc aaatagacgg cctgcgattg cttctcgctg aagatcgcga ccagcacgtt
     4981 gatcggcgtg acttccttgc ggccgctcga ttgcacgtgg aacaccgcgc gctggagaac
     5041 ccgttggaac ccgagcgtcg gctggacctc ggtcccttcc tcgtcctcgc cgcgcaggcg
     5101 cggagtggcg ccctcgatga actccttgag ctcgcggcgc aggcgcgcga gatccgcgcc
     5161 gcacgctttc aacgtttcga tcaccgtcgg cgtatcgagc aacgcgagca gcaggtgctc
     5221 gacggtgatg aattcgtgcc gctcttcacg cgctcgctgg aacgcgtcgt tcaaacaata
     5281 ttcgagctcg gtcgatagca tttacgtctc ttccatggtg caaagcagcg gatgctggtg
     5341 ctgttgtgca aggcccgtga cctgcgcgac cttcgtctcc gctatctcat atgtatagac
     5401 accgcacacg ccctttccct tggtatgcac ctcgagcatg atgcgggtcg ccgtcgttcg
     5461 gtccagagaa aaaatcttct gcagcacttc aactacgaac tccatcggcg tgtagtcgtc
     5521 gttgagcagg atcacgcggt acagcggcgg ccgctttaga cgcggtttag cttccgccgt
     5581 tgccagcccg tgatcgtcga ctcggtcacc tttttcggaa tcgtgcgaca tgtaacggga
     5641 attctatcga ccggggcggg tcaattgaac ccaagcagca acgctagagc taaggtgact
     5701 ccgacgtgcg tgagaagacg ttgacgcgtt cgacactatc aatgaattca gcgacttgtt
     5761 acgcacggag tacctgccct atcatgcccg atcgagcctg atcgcctcgg aagccaacac
     5821 ccgcatggat gtcgccaagc gactcgccga gtggagagac cgccctccgg agcagtggtt
     5881 cactacggtc aatcagtacg cgccccccgg cgtcacggcc gttctcgtca tcgcgatcgc
     5941 ctatcaactc gccacgctga cttgggcgct cgtgccgggc tcgacgcccg ccgcggtcgc
     6001 gccggcgtcg cggcccagct cgacggcaac cgcgccggcc gccgactaca gcgcgctgac
     6061 gggctcgcat ttgttcggcg aggcggccga gcaacccgaa gtggccgccg cgccggtcat
     6121 cgacgcgccc gacacgacgt tgagcctcac gctgacgggc atcctctcca agaacgagga
     6181 cgggagcggg agcgtgttga tcaagaacag ccgcaacgtt gaaaaaacgt actgggtagg
     6241 ccaggacatc gagggcgccg acggcgccaa gctgcattcg gtctacgccg atcgcgtgct
     6301 gctcgaccgc aacggccggc tcgagacgtt gcgttttccg aaggacctca ccgcgtcagc
     6361 cggctcgccg atggcgttgc cgccgttgcc gcaaacgcaa gcacagcccg ctccgctgcg
     6421 cgaagtcatc agccagaacg cctcgaagct cacggacatc atccggccgg ccccgcacgt
     6481 gcaagagggc caaatcgtcg ggttccgcgt gaacccggga cgcgatcgcg cgacgttcga
     6541 agcgttggga ttccaacccg gcgacgtcgt cacggacatc aacggcaccg tgctcgacga
     6601 cgtgagccag ggcctgcaga tcttcgaatc gctcggggaa gcgacccaag cgaacgtcac
     6661 cgtgctccgc gacggcgtac cgcaagtgct cgtcatcgac acgacccagt tgcagaagct
     6721 tcaggagaac cgccagtgaa ccactcgacc ccggacgcgc gccgcgccgg cgcgtggcgg
     6781 cgcacgcctt tacggcacgc cgcgctttgc gccgcgctct gggcgtgctt cgtcggcggc
     6841 tccgcgcgag cgcagccgca gcaaccggtg ctctacacgg cgaactatcg cgacgccgac
     6901 attcgcatgg tcacggagca ggttcagcag gtgatcgggc ggccgatcat catcgatccg
     6961 cgcgtgcgcg cgcagctgac gatcctgtcg aacgcgccga tgaccgccga tgcgttctac
     7021 cggacgtacc tcgcggcgct cgaggtccat ggcttcattc cactcgagtc cggcaacaac
     7081 atcatgatcg ttccggacgc gaatgcccgc ttcggctccg gcgaagactt cgtcacgcag
     7141 gcaatcatcc tcgacaacat cggcgcgcag cagctcgtgc cgatacttcg ccccattctt
     7201 gctcaatacg cgcatctcgc cgcacaccaa gcatcgaacg cgctgatcat cgcggatcgg
     7261 cctcagaaca ttcgccgcgt caacgagctc gtcaggcgct tggatcaagc gagcacggct
     7321 gacatcgagg tgattcagct cgaaaacgcg tcggccgacg aggtcgtccg aatgctcagc
     7381 cagctcaaca cggcggcgca ggcggccggc ggctcgccgc cgatccaggt gattccggac
     7441 ccgcgcacga acagcgtgct gctgtcgggc gccggcgccg tgaagctgca gatgcgcgcg
     7501 ttgattgcgc acctcgacac gccgtccgcg gaaggcggcg actcgctggt tcgttacctg
     7561 aactacgcgg acgccgaaga tctcgcgacg aagctgcaag cgcaattcgg cggcagcggc
     7621 ggcggtgcgc cggccacgcc ggccgaaggc gcctccccgg ccacgagcgg ccccgtgacg
     7681 atctgggcgg acaccggcac caacgcgctc gtcatcaacg ctcccgagcg cgtccgcgcc
     7741 gacatgctcg cgatcatcaa ccagatcgac attccgcgcc tgcaggtcca ggtcgacgcc
     7801 atcatcgtcg agctcagcga agagcagacc gccgcgctcg gcgtgacgtg gctgcgagcc
     7861 gacgacgagt tcatcggcct cacgaacttc gcgaatgccg cgggcggcgg catcctgggg
     7921 ctcgcgagcc aggcgggcgc cgaaaccccg aaccttgcgg ccgtcgccga gggcatctcg
     7981 gtcggcatcg gcaagctctc cggagccggc ggctgggcgg cactgctgaa cgcgctgcgc
     8041 ggcgaaggcg agacgaacat catctcgacg ccgcaaatcg tcacgctcga caacgaggag
     8101 gccgagatcc gcgtcggcca agaggtgcca ttcgtgacgg gccagttcac gaacacgggc
     8161 gcgaatcagg gctccgtgaa cccgttccaa acgatccagc gcgagcagat cggcacgaac
     8221 ttgaagatca cgccgcagat caacgagggc agcggcgtca agctcgacat cgagcaggag
     8281 acctcgagct tggccgccac cgtccaggga gcggccgacc tcgtgacgaa cacgcgaacg
     8341 atcacgacgt cggtgttcgt caacgacggc gacgtgctcg tgctcggcgg cctcatcgac
     8401 gacaagctgc tcgagtcgga tcggcgcgtg cccggcctag gcaagatccc ggggctcggc
     8461 tggttgttcc gcgcgcggac gtcggaccgc aacaagacga atctcatggt gttcatccgc
     8521 ccgacgattc tgaaaacgcc ggaggatgcg cgcttccaga cgaacacgaa gtatcgctac
     8581 atccaggatc tgcagcgcca gatggcggcg gatcccgtca tgctgatgca cgagttcgag
     8641 cggccgcagt tgccgccgtt gcccgaagag caagagctcc cgcgcccgga cgaggggccg
     8701 accgagaccg tcgtaccgaa cggcgccccg cccgatggca gcgaacctcg gtagcgacga
     8761 ccccgtagtc gaggtgcccg ttgcggctcc gtcgcagcgg aacctgccgt tcgcgttcgc
     8821 gaagcgtcac ggcgtcctga tccaacgcct gcacgacggc gccgccgaag cgatttaccg
     8881 aacgggcgtc gccgcgcaga gcatcgccga ggtcaggcgt ttcgcgggca tgcccgtgcg
     8941 attttccgag gtcgacccg
//
LOCUS       DASSNN010000504         3003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_464738, whole genome shotgun sequence.
ACCESSION   DASSNN010000504 DASSNN010000000
VERSION     DASSNN010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464738"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1734)
                     /gene="ilvA"
                     /locus_tag="VFL84_09330"
     CDS             complement(184..1734)
                     /gene="ilvA"
                     /locus_tag="VFL84_09330"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HET7608865.1"
                     /translation="MSTQPKSYPIAASYAPLIARARVYDVAIESPLDRAPRLSARLGH
                     DVLLKREDLQAVFSFKLRGSHNKLAQTPAEKLANGVICSSAGNHAQGVALAARQRGLR
                     AVIVMPKTAPSIKVDAVVALGGEAVLHGMTYDEANAHALTLERAQDLCFVHPFNDPDV
                     IAGQGTIGGELERQWPRVPDAVFVPVGGGGLISGIGAHLKHRYPGIKIVGVEPYESAS
                     MYESLRSGRIVTLDRVGTFADGVAVRRVGEETFRVASEVVDEIILVSTDEICAAIKDI
                     FEDVRVIVEPAGALSVAGMKRYAEARAARGATFVAVLSGANMNFGRLRHVTERAEIGE
                     HREALLAVEIPERPGAFLKFCEALGARNITEFNYRYVPGATARIFVGVSLQRGAAEAA
                     EIVAVLQAQGYPVLDLSGNELAKLHIRHLVGGQVPGLTNELLYRFEFPERPGALLSFL
                     QAVGDLWNISLFHYRNHGSDYGRVLAGVQVPETERVEFARHLAKIGYTYSDETANPAY
                     RLFLHSGS"
     gene            complement(1788..2855)
                     /locus_tag="VFL84_09335"
     CDS             complement(1788..2855)
                     /locus_tag="VFL84_09335"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HET7608866.1"
                     /translation="MSRRTVAILAAALVVLLALVIFGQRTSAPVGTGAALVPDLAAAL
                     GDLERVTIVKANGETVATLEKRPDNWVVADKHGYAADATKLRQALTALSEAKILEQKT
                     ANPDLYARLGVEDVTGANAAGVSVAFTAQGHELPTIILGNAEGSKYRYARRAGEAQSY
                     LIDRNPDVPRAAAQWVDSQIVDVRGERIREVTITHADGEVVHLSKASSELANFDVADV
                     PKGRELSYPGVANVIGNSLRELNLEDVEPASAPADQPTVVEFRTFDGLVVRVTGVKRG
                     DEDWITLEASVDAEQAAANAPPAAPAEGAAASTTPPPDPNAEAQRINAKVSGWRYKIA
                     GYQYDQMTRRMADLLKAVPPA"
BASE COUNT          453 a         1022 c         1049 g          479 t
ORIGIN      
        1 ccgcttcaga cgatgctcgc agtcgtcgat cagctcgtcg ccgaagcgag gacgtgcaag
       61 ccgctcacat tgataactga gcccaccagc atcgcggtgc agcgcaacgt ctgccccgag
      121 gcagcgcggc agcgctgagc cgcggccgag ccggcgcacg atgagctcga cgcatgagtc
      181 ccgtcatgag ccggagtgca ggaacagccg ataggccggg ttcgccgtct cgtccgagta
      241 cgtgtagccg atcttcgcga gatgccgcgc gaattcgacg cgctcggtct cgggcacttg
      301 cacgcccgcg agcacgcggc cgtagtcgga gccgtgattg cggtaatgga acaggctgat
      361 gttccagaga tcgccgacgg cttggagaaa cgagagcagg gcgccggggc gctccgggaa
      421 ctcgaaccga tagagcagct cgttcgtgag ccccggcact tggccgccga cgagatgccg
      481 gatgtgcagc ttcgcgagct cgttgccgct caagtcgagc accggatagc cttgcgcttg
      541 cagcacggcg acgatctcgg cagcttcggc cgcgccccgc tgcaacgaca cgccgacgaa
      601 gatgcgggcg gtggcgcccg gcacgtagcg gtagttgaac tccgtgatgt tgcgagcgcc
      661 gagcgcctcg cagaacttca agaacgcccc gggccgctcc ggaatctcga ccgcgagcag
      721 cgcctcgcgg tgctcgccga tctcggcgcg ctccgtcacg tggcgcaacc ggccgaagtt
      781 catgttcgcg ccgctcaaca cggccacgaa cgtcgcgccg cgcgcggcgc gagcttccgc
      841 atagcgtttc atgccggcga cgctgagcgc gccggccggc tcgacgatga cgcgcacgtc
      901 ctcgaagatg tccttgatcg cggcgcagat ctcgtccgtg ctcacgagga tgatctcgtc
      961 gacgacctcg ctcgccacgc ggaacgtctc ctcgccgact cggcgcacgg cgacgccgtc
     1021 ggcgaacgtg ccgacacgat cgagcgtgac gatgcgccct gagcgcagcg actcgtacat
     1081 gctcgccgat tcgtacggct cgacgccgac gatcttgatg ccggggtagc ggtgcttcaa
     1141 gtgagcgccg atgccgctga tcagcccacc cccgccgacg ggcacgaaga cggcgtccgg
     1201 cacgcgcggc cattgccgct cgagctcgcc gccgatcgtg ccttggccgg cgatcacgtc
     1261 gggatcgttg aacgggtgca cgaaacagag gtcctgcgcg cgctcgagcg tgagcgcgtg
     1321 cgcgttcgct tcgtcatagg tcatgccgtg cagcaccgcc tcgccgccga gcgcgacgac
     1381 ggcgtcgacc ttgatcgaag gggccgtctt cggcatcacg atgacggcac gcaggccgcg
     1441 ctgccgcgcg gcgagcgcga cgccctgcgc gtggtttccg gccgagctgc agatgacgcc
     1501 gttcgcgagc ttctccgcag gggtctgcgc gagcttgttg tgcgagccgc gcagcttgaa
     1561 cgagaacacg gcttgcaagt cttcgcgctt aaggagcacg tcgtggccga gccgcgccga
     1621 cagccgcggc gcgcgatcga gcggcgactc gatcgcgacg tcgtagacgc gagcgcgcgc
     1681 gatcaacggc gcgtagcttg cggcaatcgg atacgacttc ggctgcgtgc tcatggccta
     1741 cccgcggagc ggaaggcacg cagtgtaccg ggccgcggcg gcggccgtca ggctggcggc
     1801 acggccttga gcaggtccgc catgcgccgc gtcatctggt cgtattggta tccggcgatc
     1861 ttgtagcgcc aaccggagac tttcgcgttg atgcgctgcg cctcggcgtt cgggtcgggg
     1921 ggcggcgtag tcgaggccgc ggcgccttcg gccggagcgg ccggcggcgc gttcgcggcc
     1981 gcttgctcgg cgtcgacgct ggcctcgagc gtgatccaat cctcgtcgcc gcgcttgacg
     2041 cccgtcacgc gcacgacgag cccgtcgaag gtccgaaact cgacgaccgt cggttggtca
     2101 gcgggcgccg aagcgggctc gacgtcctcg agattgagct cccgcaatga gttgccgatc
     2161 acgttcgcga cgcccggata cgagagctcg cgtcccttcg ggacgtccgc gacgtcgaag
     2221 ttcgcgagct cggagctcgc cttcgagaga tgaacgacct cgccgtcggc gtgtgtgatc
     2281 gtgacctcgc ggatgcgctc gccgcggacg tcgacgatct gcgagtcgac ccattgcgcg
     2341 gccgcgcgcg gcacgtccgg gttgcgatcg atcaggtagc tctgcgcctc gccggcgcgg
     2401 cgcgcgtagc gatacttcga tccttccgcg ttgccgagga tgatcgtcgg cagctcgtgg
     2461 ccctgcgccg taaacgcgac gctgacgccc gcggcgttcg cgcccgtgac gtcctcgacg
     2521 ccgaggcgcg cgtacaggtc gggattcgcg gtcttctgct cgaggatttt tgcctcagag
     2581 agcgcggtca gcgcttgccg cagcttcgtc gcgtcggcgg cgtagccgtg cttgtcggcg
     2641 acgacccagt tgtcgggccg cttctcgagc gtcgcgacgg tctcgccgtt cgccttgacg
     2701 atggtcacgc gctcgagatc gccgagcgcg gcggcgaggt ccggaaccaa cgcggcgccg
     2761 gtgccgaccg gtgccgacgt gcgctgcccg aagatgacga gcgcgagcag cacgacgagc
     2821 gctgcggcga gaatcgcaac ggtgcgccgg ctcatcggcg tgccttcctg ttgcggcgca
     2881 tcagcacgac gccgagcgcg gcaagcgtca gcagcacggg catcagcacg atgttgatcg
     2941 ccttgagcca ggttccgagc cgctcgatgt ccgcatcgag gttgcggcgc acggcgcgca
     3001 gct
//
LOCUS       DASSNN010000505         2934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_681186, whole genome shotgun sequence.
ACCESSION   DASSNN010000505 DASSNN010000000
VERSION     DASSNN010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681186"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1426
                     /locus_tag="VFL84_09340"
     CDS             65..1426
                     /locus_tag="VFL84_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009024915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HET7608867.1"
                     /translation="MKKITCFKAYDVRGRVPEELDEDIAYRIGRGYAAFVKPRVVAVG
                     RDIRLSSPMLTAALIKGLTDSGVDVLDIGVGGTELSYFATFSRELDGGIMVTASHNPP
                     NYNGMKFVREGSRPISADTGLQEIRAFAEADELGPPAPKRGSVKQVDVKPAYIEHLLS
                     YVDVKSLAPLKIVLNAGNGGAGPIVDLLEPHLPFRFVKLFHEPDGTFPNGVPNPMIEE
                     SRKVTIERVRAERADLGIAWDGDYDRCFLFDELGQFIEGYYIVGLLAQSFLERSKGER
                     IVHDPRLTWNTLDIVSRLGGKAVQSKSGHAFIKQRMREVDAVYGGEMSAHHYFRKFSY
                     ADSGMIPWLLIAQIMSERKASLSELVGERMRAFPASGEINRKVKDAREVIARVEAKYT
                     PQAIAVDRTDGLSIEFPRWRFNLRSSNTEPLLRLNVESRGDAELMRAKTAELLALLGG
                     EPG"
     gene            1491..>2934
                     /locus_tag="VFL84_09345"
     CDS             1491..>2934
                     /locus_tag="VFL84_09345"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7608868.1"
                     /translation="MPDALRDPRCYGRGVNNVRLVETHISWVFLTGRFAYKVKKPVKL
                     PFVDFSTLKLRKRFCDEELRVNRRFAPELYLRVVPIGGTPAAPRIGRTPAFEYAVKMR
                     EFPADARLDRRLAANAVPRTALGDFGARLAQLHAELPRARGLPADEIGAAALRNVDEL
                     EAALQGRHGKALATLRTWTKRQGATLEQVFAQRAAAGARRECHGDLHLQNLLWRNGKI
                     VAFDALEFDRKLRDVDVVSEIAFLAMDLHAHDRLDLAYELKSRYFETGGDYGGVDVLP
                     FYLVYRALVRAKVAAIKQAQSAADGHEAARYLETALELAARRKPLLVITHGFSGSGKT
                     TITDELVSRLPAIRARSDLERKRLHGLAPAARSGSGLGTGLYAPSASRRTYAALAEIA
                     DRLLRNGQNALIDATFLRRDERLQFRQIAASNAARFAILDCTAPPDELRRRIRARARR
                     GRDASEADLEVLERQLSTAEPLDRAERRHVV"
BASE COUNT          504 a         1008 c          972 g          450 t
ORIGIN      
        1 gactagctgg catcattaca cagtagcgcg ggcgttcagc tcgccaaaga cagccggatc
       61 cacgatgaag aaaatcactt gtttcaaagc ctacgacgtt cgcggccggg tgcccgagga
      121 gctagacgag gacatcgctt atcgcatcgg ccgcggttat gccgcattcg tgaagccgcg
      181 cgtcgtcgcg gtcggcaggg acatccgcct gtcgagcccg atgctgaccg cggctctgat
      241 caaagggctc accgactccg gagtcgacgt gctcgacatc ggcgtcggcg gcacggagct
      301 gtcctatttc gcgacgttca gccgcgagct cgacggcggc atcatggtca ccgcgagcca
      361 caacccgccg aactacaacg gcatgaaatt cgtgcgcgaa gggtcgcggc cgatcagcgc
      421 cgacaccggc ctgcaggaga tccgcgcatt cgcggaggcc gacgagctcg ggccgccggc
      481 gccaaaacgc ggtagcgtca agcaagtcga cgtcaaaccg gcgtacatcg agcacctgct
      541 ttcgtacgtc gacgtgaaat cgctcgcgcc gctcaagatc gtcctgaacg ccggcaacgg
      601 cggcgccggg cccatcgtcg acctgctcga gccgcatttg ccgtttcgct tcgtcaagct
      661 cttccacgag cccgacggca cgttcccgaa cggcgtgccg aacccgatga tcgaggagag
      721 ccgcaaggtc acgatcgaac gcgtccgcgc cgagcgcgcc gatctcggga tcgcgtggga
      781 cggcgactac gaccgctgtt tcttgttcga cgagctcggc cagttcatcg agggctacta
      841 catcgtcggc ttgctcgcgc aatcgttcct ggagcgctcg aagggcgaac gcatcgtgca
      901 cgacccgcgc ctgacgtgga acacgctcga catcgtcagc cgcctgggcg gcaaggcggt
      961 gcaaagcaag tccgggcacg cgttcatcaa gcagcgcatg cgcgaggtcg acgccgtcta
     1021 cggcggcgag atgagcgccc accattactt ccgcaaattc tcgtacgccg acagcggcat
     1081 gatcccgtgg ctcttgatcg cgcagatcat gagtgagcgc aaggcgtcgt tgtcggagct
     1141 cgtcggcgag cgaatgcggg cctttcccgc gagcggcgag atcaaccgca aggtcaagga
     1201 cgcgcgcgag gtcatcgcac gcgtcgaggc caagtacacg ccgcaagcca tcgcggtcga
     1261 tcgcaccgat ggattgagca tcgaattccc gcggtggcgg ttcaatctgc gctcgtcgaa
     1321 cacggagcct ttgctccgcc tcaatgtcga gagccgcggc gacgccgagt tgatgcgagc
     1381 gaaaaccgcg gagctgctcg cgctgctcgg cggagaaccg ggatagcgtg cgccgcaaag
     1441 cgcgaccgcc acggacggag ggccgcccgg aggagccgaa acagcgctcg ttgcctgacg
     1501 cgctgcgcga tccgcgctgc tacggccgcg gcgtaaacaa cgtccgcctc gtggagacgc
     1561 acatctcgtg ggtcttcttg accgggcgct tcgcctacaa ggtcaagaag cccgtcaagc
     1621 tgccattcgt cgacttcagc acgctgaagc ttcgcaagcg cttctgcgac gaggagctgc
     1681 gagtgaatcg gcgcttcgcg ccggagctct atctgcgcgt cgtgccgatc ggcggcacgc
     1741 cggcggcgcc gcgaatcggc cgcaccccgg ccttcgagta cgccgtgaag atgcgcgagt
     1801 ttccggccga cgcgcgccta gaccggcggc tcgccgcgaa cgccgtgccg cggaccgcgc
     1861 tcggcgactt cggcgcgcgg ctcgcgcagc tgcacgcgga gctgccgcgc gcgcgcggcc
     1921 tccccgcgga cgagatcggc gccgccgctc tgcgcaacgt cgacgagctc gaggccgcgc
     1981 tgcaagggcg tcacggcaag gcgctcgcga ccttgcgcac ctggacgaag cgccagggcg
     2041 cgactctcga gcaagtgttc gcgcagcggg cggccgccgg cgcgcggcgc gagtgtcacg
     2101 gcgatctgca tttgcagaat ctcctgtggc gcaacggcaa gatcgttgcc ttcgacgcgc
     2161 tcgagttcga ccgcaagctt cgcgacgtcg acgtcgtcag cgagattgcg ttcctcgcca
     2221 tggatttgca cgcgcacgat cgcttggatc tcgcctacga gctcaagagc cgctatttcg
     2281 agaccggcgg cgactacggc ggagtcgacg tgctgccctt ctacttagtc taccgcgcgc
     2341 tcgttcgcgc caaagtcgct gcgatcaagc aagcccagtc ggcggccgac gggcacgaag
     2401 cggcgcgcta tctcgagacc gccctcgagc tcgcggcccg caggaagccg ctgctcgtca
     2461 tcacgcacgg cttttcgggc agcggcaaga cgaccataac cgacgagctc gtgagccgct
     2521 tgcccgccat tcgcgcccgc tccgacctcg agcgcaagcg gctgcacggg ctcgcgccgg
     2581 cggcgcgctc gggctcgggg ctcggcacgg ggttgtacgc gccctcggcc agccgaagaa
     2641 cttacgccgc gttggcggag atcgccgatc ggctgctccg caacggccaa aacgcgctga
     2701 tcgatgcgac gtttctgcgg cgcgacgagc ggctgcaatt tcgccaaatc gccgcatcga
     2761 acgcggcgcg gttcgcgatc ctcgactgca ccgcgccgcc cgacgagctg cggcgccgca
     2821 ttcgcgctcg cgcgcgcagg gggcgcgacg cgtcggaggc cgatctcgag gtcctcgagc
     2881 ggcaactttc gacggccgag cccctggatc gcgcggaacg gcggcacgtc gtgg
//
LOCUS       DASSNN010000506         9212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_321570, whole genome shotgun sequence.
ACCESSION   DASSNN010000506 DASSNN010000000
VERSION     DASSNN010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321570"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFL84_09350"
     CDS             complement(<1..582)
                     /locus_tag="VFL84_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HET7608869.1"
                     /translation="MLRTTLVGVAHWLVAGTAFAQELHIPRVAEPPKLADFVSMAPAE
                     EVRSRYAVVTDFTQREPQNGAPSSQRTDVYLGYDSKNLYAVFVAHDDDPKSVRANLAP
                     RENVGNDDRVGLLVDTFDDQRTAYAFRSTPLGVQWDARWAELGREGFDSSFEAVWYTD
                     AQLTAGGYVVLMTIPFRAMRFPETLEQRWRIQFE"
     gene            complement(649..1128)
                     /locus_tag="VFL84_09355"
     CDS             complement(649..1128)
                     /locus_tag="VFL84_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608870.1"
                     /translation="MPKYIAIALAGLAAFTAAQPPAALGQGGLDRRLQAAKRIECTFS
                     VLGTGTWDKNVPAITVTPAEIKANFFDINIDEGTAEAESAYGASFISVRYESGYLHIM
                     QMSDAGPLYLTTVLARAAGEGKLMAVHTRIEYSPTVIPGFTSRPEMYLGTCTIANPG"
     gene            1227..1802
                     /locus_tag="VFL84_09360"
     CDS             1227..1802
                     /locus_tag="VFL84_09360"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HET7608871.1"
                     /translation="MKTLLTRLAALAVGAALLAVGASGQSSNDAELTAQLKKLFPNAA
                     TFSTKSGNPAHYKAFGPAAGGKEGELLGLAYWTTELEPLERGFDGPIKMLVGLNPSGE
                     ITGVVVTDHREPYGYFSVDLPSWPKQFEGKSIRDSFKVGADVDAISRATITVTSASRA
                     IRNSSRKIARAYLAPPETPSSGAAAPAAAAQ"
     gene            1799..2887
                     /locus_tag="VFL84_09365"
     CDS             1799..2887
                     /locus_tag="VFL84_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET7608872.1"
                     /translation="MSSIRALLARRGVYALLLCMLVLGLGASAQPPADEPAAGAAAEA
                     PAWSFDDEEEEAAPTLLQLAREQALDIALFVAFAALVMVSFLRKSVALKYVTLVAAVL
                     YMGIYKSQLISIVNVFGVLGGGVSAASIGLPGGEPATAAAIGGALKSSLPPFAYSLAW
                     YTFAVFAVVTTIVWGRVYCGRVCAFGALTQLMDAVFPKRFQLEIPAKLEARASYVKYG
                     ILLGAIALYFATKEIAFYRYIEPFWLFTFDATPVLWVMVGVLLVASLFVRNLYCRFLC
                     PLGAALGLISKATTVLPIKRWSECSQCALCEKTCEWGAIRKRQILKTECVRCDDCEIL
                     YDDKARCPHWLLELKRKTRDTVVGKVTA"
     gene            complement(2877..3143)
                     /locus_tag="VFL84_09370"
     CDS             complement(2877..3143)
                     /locus_tag="VFL84_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET7608873.1"
                     /translation="MASYDLVFKKSVAKDLREFPKADVKRILQRIRSLADDPRPAGSE
                     KLSGRERYRLRQGIYRIVYEIDDGVLTVLVVKIGHRRDIYRTKQ"
     gene            complement(3130..3366)
                     /locus_tag="VFL84_09375"
     CDS             complement(3130..3366)
                     /locus_tag="VFL84_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET7608874.1"
                     /translation="MSDEASKRATIYFEPALHRAIRLKAAHTNRSLSEIVNDAVRWSL
                     REDQEDLAAFEDRVEEPTMSYEALLKDLKAHGKL"
     gene            3566..5185
                     /locus_tag="VFL84_09380"
     CDS             3566..5185
                     /locus_tag="VFL84_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016486587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HET7608875.1"
                     /translation="MISAANVTIQFGEKPLFEDVSVKFGNGNRYGLIGANGSGKTTFL
                     KILAGELEPTHGNVSIEPNVRVGKLRQDQFAFEQVPVLHTVIMGHERLWQLYQERERL
                     YSKPSLSEAEGLHAAEVESEFAALDGYSAESAAGELLLAVGIPENLHQGPMSAVAPGW
                     KLRVLLAQALFADPDVMLLDEPTNNLDIDTIRWLEGVLTQRKCTMVIISHDRHFLNTV
                     CTHMADLDYGELRLFPGNYDEYMIASTQAREQMLADNAKKKAQIADLQAFVSRFSANA
                     SKSRQATSRKRQIEKIELAEVKPSSRQSPYIRFEQDKKLYRLAVSLDKLSKSYGDHKV
                     FRDVTLHVEAGERVAVVGTNGIGKTTLLRSIMAELPVNHGTVKWAENAEVAYMPQDHA
                     AAFEEPATVFDWMRARGKPRDDDQAIRATLGRLLFSRDEMQKTVDKLSGGERGRMLFG
                     EIMLKRPNIILMDEPTNHLDMESIEALNLALENYPGTLIFVSHDREFVSSIATRVVEI
                     KSTGVIDFRGTYDDYLRSRGIAIDLQRAASQ"
     gene            5190..6194
                     /locus_tag="VFL84_09385"
     CDS             5190..6194
                     /locus_tag="VFL84_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7608876.1"
                     /translation="MARRDPNAPRAPDPASLVADLSRAHIAARCLHVIAECGAADALG
                     DAGASAAEIAAQIGLDADALDRMLRLLAAHGIFVRTADGRYAHSAASRLLKTDEPGSL
                     RSYVRMAGMPAFWDRFTELGRTAREGQGRHDMTALVDYYAAHPEEAAIFNAAMVSKSR
                     TVLPAVAAAYDFEPYSTIVDVGGGRGHLLKLILERAPNAKGVLFERPHVIEDTQPAQR
                     LELVGGDFFVDALPEGDLYLLMDLLHDWDDADAARILTAVRRAARPRSRVLIIETLVP
                     EIPGPHFGKTLDIIMLAVTRGRERTEAQHAALLTAFGFELTRVLPTASQYSIVEAVAV
                     "
     gene            complement(6191..7714)
                     /locus_tag="VFL84_09390"
     CDS             complement(6191..7714)
                     /locus_tag="VFL84_09390"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7608877.1"
                     /translation="MSRTASPSPWLPEPMAVAPALDPLAIPGPRRKRYALLLNPFYRK
                     DPHASFGKHVLTPTLALTAIAGATPDRWQLGYWDENLLQGPPPLEPFPEVVAITVHLT
                     FAKRAYELAQWYRQWGAKVVFGGLHVLSAPEECAPHADAIALGEGVQVWPQILADVEA
                     GTLKPVYRGDYRKPYRDDPAPRRDLLPRDGFLTTSSVIATRGCHNRCGFCYLSTEGLH
                     MPYRLRDVEQVVAELKADGQPYAVFVDNNLGSRPDYLRELCRALRPLDMIWSAALTID
                     VTDDPSLVREMALAGCTGVFIGFESLNAGNIRDAGKRSPVPDDYARRVAILHDNGIQV
                     NGSFVLGFDHDDATVFEKTVEWVEQNRLECATFHILTPYPGTPLFEQLEKEKRIVHRD
                     WRLYDTAHVVFEPKLMSGEELMAGYERCYRRLFSHRSIWRRRPKDWRAVLPYLAMSYL
                     YKRSNRIWHFLIRYRLTALVWRPLVELTRLRHLRFRRRLAQRDARVPEVRTVVSAGV"
     gene            7872..8054
                     /locus_tag="VFL84_09395"
     CDS             7872..8054
                     /locus_tag="VFL84_09395"
                     /inference="COORDINATES: protein motif:HMM:NF022894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE-like protein"
                     /protein_id="HET7608878.1"
                     /translation="MRAMRERRRARGLRELRLVVPDARSKAVRRRVAKAVAALDRSSE
                     IDAMKWIEAVSEFDTR"
     gene            8051..8380
                     /locus_tag="VFL84_09400"
     CDS             8051..8380
                     /locus_tag="VFL84_09400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HET7608879.1"
                     /translation="MKRGDVVTVAATGDYGKPRPAVIVQTDALPAEHASVVVCQMTSE
                     LSVAADFRVNIEPTESNGLRAPFQVMADKPVTIRRERIGKRVGRLDEADIARLNVALA
                     FVMGLAD"
     gene            complement(8442..8909)
                     /locus_tag="VFL84_09405"
     CDS             complement(8442..8909)
                     /locus_tag="VFL84_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7608880.1"
                     /translation="MALNTKKVIDQLNRILECELAGVVRYTHYSMMIFGYSRIPIVKW
                     FQDEAAESLQHAHQAGEMVTRLGGHPSLAIGDLLETHAHDIGQILRETLQAEHYALGL
                     YMELLKLVEGSSVALEEYARDLITQEEAHIDEIDKMLRKPGETTISKEARELD"
     gene            complement(8932..>9212)
                     /locus_tag="VFL84_09410"
     CDS             complement(8932..>9212)
                     /locus_tag="VFL84_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET7608881.1"
                     /translation="EMSAANLAKTPEPNTRLHHLRRFYYDTAGATNPINMQALKTLVG
                     MSQIVFGTDAPFFDGAPQLRGLQSAGFTAQELARVERANALAFLPRFA"
BASE COUNT         1576 a         3013 c         3044 g         1579 t
ORIGIN      
        1 ctcgaactgg atgcgccaac gctgctcgag cgtctcgggg aagcgcatcg cgcgaaacgg
       61 aatcgtcatc aagacgacgt atccgccggc cgtaagctgc gcgtcggtgt accagaccgc
      121 ttcgaacgac gagtcgaaac cttcgcggcc tagctcggcc cagcgcgcgt cccactgcac
      181 tccgagtggg gtcgagcgga acgcatacgc ggtccgttgg tcgtcgaacg tgtcgacgag
      241 caggccgaca cggtcgtcgt tgccgacgtt ctcgcgcggc gcgagattcg cgcgcacgct
      301 cttggggtcg tcgtcgtgcg cgacgaacac ggcgtagaga ttcttcgagt cgtagccgag
      361 atagacgtcg gtgcgctggc tcgaaggcgc gccgttctgc ggctcgcgct gcgtgaagtc
      421 cgtcacgacg gcgtagcggc tgcgcacttc ctcggccggc gccatgctca cgaagtcggc
      481 gagcttgggc ggctcggcga cgcgcggaat gtgcagctcc tgcgcgaatg ccgtgccggc
      541 gacgagccaa tgagcgacgc ctacgagcgt cgtccgtaac acaggcaagc gcgcaacact
      601 cggcattctg ataagccttg gactcgcacg gagcgagtag aaaaactact agccgggatt
      661 cgcgatcgtg caggtgccca agtacatctc gggccgcgac gtgaaccccg gaatcactgt
      721 cggcgaatac tcgatgcgcg tgtgcacggc catgagcttg ccctcgccgg ccgcgcgcgc
      781 gagcacggtc gtcaggtaca acgggcccgc atcgctcatc tgcatgatgt gcaagtaccc
      841 cgactcgtag cgcacggaga tgaacgaggc gccgtacgcg ctctcggcct cggccgtgcc
      901 ttcgtcgatg ttgatgtcga agaaattcgc cttgatttcc gcgggcgtca cggtgatggc
      961 cgggacgttc ttgtcccagg ttcccgtgcc gagcacggag aacgtgcact cgatgcgctt
     1021 cgcagcctgc agcctgcggt ccaggccgcc ttgacccaat gccgcgggtg gctgcgccgc
     1081 ggtgaacgcg gccagaccgg ccagtgcgat cgcaatgtac ttcggcattg ctccgtattg
     1141 tgcatgggca ccaaaggctt ggccacgcgg cgtaaagcaa acttgaccgt tgctcgcctc
     1201 gcccagcgcc cggataatcg agcccgatga agactttact cacgcgcctc gcggcactcg
     1261 cggtcggcgc ggctctcctc gcggtcggcg caagcggcca gagctcgaac gacgccgagc
     1321 tcacggcgca gctcaagaag ctgtttccaa acgccgcgac gttctctacg aagagcggca
     1381 atcccgcgca ctacaaagcg ttcgggcccg cggccggcgg caaagaaggc gagctgctcg
     1441 gcctcgccta ctggacgacg gagctcgagc cgctcgagcg gggcttcgac ggcccgatca
     1501 agatgctcgt cggcttgaat ccgagcggcg aaatcacggg cgtcgtcgtc acggatcatc
     1561 gcgagccgta cggctatttc tccgtcgatt tgccgagctg gccgaagcag ttcgagggca
     1621 agagcattcg cgactcgttc aaggtcggcg ccgacgtcga cgcgatctcg cgcgcgacga
     1681 tcacggtgac gagcgcgagc cgcgcgatcc gcaactcctc gcgcaagatc gcgcgcgcat
     1741 acttggcgcc gcccgaaact ccgtcctccg gcgctgcggc ccccgccgcc gcggcccagt
     1801 gagctcgatc cgagcgttac tcgcacgccg cggcgtgtac gcgctgctgc tttgcatgct
     1861 cgtgctcggc ctaggcgcca gcgcacaacc gccggcggac gagccggccg ccggcgcggc
     1921 ggccgaagcg cccgcgtgga gcttcgacga cgaggaagag gaagctgcgc cgacgctgct
     1981 tcagctcgcg cgcgagcaag cgctcgacat cgcgttgttc gtcgcgttcg cggcgctcgt
     2041 gatggtgagc ttcctgcgca agagcgtcgc gctgaaatac gtgacgctcg tcgcggccgt
     2101 gctctacatg ggcatctaca agagccaact catctcgatc gtcaacgtgt tcggcgtgct
     2161 tggcggcggc gtgtcggccg cgagcatcgg cttgcccggc ggagagcccg ccacggcggc
     2221 ggccatcggc ggcgcgttga agagcagcct gccgccgttc gcgtacagcc tcgcgtggta
     2281 cacgttcgcc gtcttcgccg tcgtcacgac gatcgtctgg ggccgtgtct actgcggacg
     2341 cgtctgcgcg ttcggcgcgc tgacgcagct catggacgcc gtcttcccga agcgcttcca
     2401 gctcgagatc ccggcgaagc tcgaggcgcg cgcgagctac gtcaagtacg gcatcctgct
     2461 cggcgctatt gctctgtatt tcgcgacgaa agagatcgcg ttctaccgct acatcgagcc
     2521 tttttggctg ttcacgttcg acgcgacgcc cgtgctctgg gtcatggtcg gcgtgctgct
     2581 cgtcgcgagc ctgttcgtgc gcaatctgta ctgccgtttc ctctgcccgc tcggcgcggc
     2641 gctcggcttg atctcgaaag cgacgacggt gctgccgatc aagcgctggt ccgagtgcag
     2701 ccagtgcgcg ctctgcgaga agacctgcga gtggggcgcg atccgcaagc ggcagatcct
     2761 gaagaccgag tgcgtgcgct gcgacgactg cgagatcctt tacgacgaca aagcacgctg
     2821 cccgcactgg ctgctcgagc tgaaacgcaa gactcgcgac acggtcgtcg gcaaggtcac
     2881 tgcttagtgc ggtagatgtc ccgccggtga ccgatcttca cgacgaggac ggtcaggacc
     2941 ccgtcgtcga tctcatagac gattcggtag atgccctggc gcaatcgata gcgttcccgg
     3001 cccgacagct tctcggagcc cgccggccgc ggatcgtcgg ccagcgaccg gattcgctgc
     3061 aagatccgct tgacgtcggc tttcgggaat tcgcggagat ctttggctac ggacttcttg
     3121 aaaacgaggt catagcttgc catgggcctt gaggtccttg agcagcgctt cgtaggacat
     3181 cgtcggctcc tcgacacggt cttcgaatgc cgcgaggtcc tcctgatcct cccgcagcga
     3241 ccaacgaacg gcgtcgttga cgatctccga caacgaccga ttcgtatgcg ccgccttgag
     3301 ccgaatagcg cggtgtaagg ccggctcgaa atagatcgtt gctctcttcg acgcttcgtc
     3361 gctcacgtgt cagctccttg acgttttgac gtcaaagcac tatggagccg aggccgaccg
     3421 ccgtcaatgc gagatttctc gcgagatgct taatttgcgc gcacttctcg cgagcgacga
     3481 caatgactta tactgcgcgc ccttcgcggc cgggcacagg gattgcagtg ccccggtctt
     3541 acccactcca cgtaacggga aagaaatgat ttccgccgcc aacgtcacta ttcaattcgg
     3601 cgagaagccg ctcttcgagg acgtctcggt caagttcggc aatggcaacc gttatggcct
     3661 catcggcgcg aacggctcgg gcaagacgac gtttttgaag attctcgccg gcgagctcga
     3721 gccgacgcac ggcaacgtct cgatcgagcc gaacgtgcgc gtcggcaagt tgcgtcagga
     3781 ccaattcgcg ttcgagcagg ttcccgtcct ccacaccgtg atcatgggtc acgagcggtt
     3841 gtggcagctc tatcaagagc gcgagcggct ctattcgaag ccgtcgttga gcgaggccga
     3901 aggcctgcac gcggcggaag tcgagagcga gttcgcggcg ctcgacggtt actcggccga
     3961 gtcggcggcc ggcgagctgc tgctcgcggt cggcattccg gagaacttgc atcaagggcc
     4021 gatgagcgcc gtcgcgccgg gctggaagct gcgcgtgctg ctcgcgcagg cgctgttcgc
     4081 ggaccccgac gtcatgctgc tcgacgagcc gacgaacaac ctcgacatcg acacgatccg
     4141 ctggctcgag ggcgtgctca cgcagcgcaa gtgcacgatg gtcatcattt cgcacgatcg
     4201 gcacttcttg aacaccgtct gcacgcacat ggcggacctc gattacggcg agctgcgcct
     4261 gttccccggc aactacgacg agtacatgat cgcctccacg caggcacgcg agcagatgct
     4321 tgccgacaac gcgaagaaga aagcgcaaat cgctgatcta caagcgttcg ttagccggtt
     4381 ctcggcgaac gcctcgaagt cgcggcaggc gacctcgcgc aagcgccaga tcgagaagat
     4441 cgagctcgcc gaggtcaagc cgtcgagccg gcagagcccg tacatccgct tcgagcagga
     4501 caagaagctc taccgcctcg cggtcagcct cgacaagctc agcaagagct acggcgatca
     4561 caaggtgttc cgcgacgtca cgctgcacgt cgaggccggc gagcgcgtcg ccgtcgtcgg
     4621 cacgaacggc ataggcaaga cgacgctgct gcgctcgatc atggccgagc tgcccgtcaa
     4681 ccacggcacc gtgaagtggg ccgagaacgc cgaggtcgcg tacatgccgc aggaccacgc
     4741 ggccgcgttc gaggagccag ccacggtgtt cgactggatg cgcgcacgcg gcaagccgcg
     4801 cgacgacgac caggcgatcc gcgcgacgct cggccggcta ttgttcagcc gcgacgaaat
     4861 gcagaagacc gtcgacaagc tctcgggcgg cgagcgcggc cgcatgctgt tcggcgagat
     4921 catgctgaag cggccgaaca tcatcctcat ggacgagccg acgaaccacc tggacatgga
     4981 gtcgatcgag gcgctgaacc tcgcgctcga gaactatccg ggcacgctga tcttcgtgag
     5041 ccacgaccgc gagttcgtct cgtcgatcgc gacgcgcgtc gtcgagatca agtcgacggg
     5101 cgtcatcgac ttccgcggca cctacgacga ctacctgcgc agccgcggca tcgcgatcga
     5161 tttgcagcga gccgcgagcc agtagcgtca tggctcggcg cgatccgaac gcaccgcgcg
     5221 cgccggatcc cgcttcgctc gtcgccgacc tttcgcgcgc gcacatcgcg gcgcgttgcc
     5281 tgcacgtgat cgcggagtgc ggcgccgcgg atgcgcttgg cgacgcgggc gcgagcgccg
     5341 ccgagatcgc ggcgcagata ggtctcgacg ccgacgcgct cgaccgcatg ctgcggctgc
     5401 tcgcggcgca cggcatcttc gttcgcacgg ccgacggccg gtacgcgcat tcggccgcgt
     5461 cgcgcctact caagacagac gagccgggct cgctgcgctc gtacgtgcgc atggccggca
     5521 tgccggcgtt ctgggaccgc ttcaccgagc tcggccgcac ggctcgcgaa ggccagggcc
     5581 ggcacgatat gacggccctc gtcgactact acgcggcgca cccggaagaa gccgcgatct
     5641 tcaacgcggc gatggtcagc aagtcgcgca cggtgctgcc cgcggtcgcg gcggcgtacg
     5701 acttcgaacc ctactcgacg atcgtcgacg tcggcggcgg ccgcggccac ttgctgaagc
     5761 tgatcctcga gcgtgcgccg aacgcaaaag gcgtgctgtt cgagcggccg cacgtcatcg
     5821 aggacacgca gcccgcccag cgcctcgagc tcgtcggcgg ggatttcttc gtcgacgcgc
     5881 tgcccgaagg cgatctctac cttctcatgg acctgctgca cgactgggac gacgccgacg
     5941 cggcgcgcat cctgaccgcc gtacgccgcg cggcgcggcc gcggtcgcga gtgttgatca
     6001 tcgagacgct cgtacccgag ataccggggc cgcatttcgg caagacgctc gacatcatca
     6061 tgctggccgt cacgcgcggc cgcgagcgca ccgaggcgca gcacgccgcg ctgctcacgg
     6121 cgttcggctt cgagctcacg cgcgtgctgc cgacggcgtc gcagtactcg atcgtcgaag
     6181 ccgttgccgt ctagacaccg gcgctgacga cggtgcgcac ctcgggaacg cgcgcgtcgc
     6241 gctgcgcgag ccgccgcctg aaccgcaagt gccgcaggcg cgtcagctcg acgagcggcc
     6301 gccagacgag cgccgtcagg cggtagcgga tcaagaagtg ccagatccgg ttcgagcgct
     6361 tgtagagata cgacatcgcg agatacggca gcacggcgcg ccaatctttc gggcggcggc
     6421 gccagatcga gcggtgcgag aagaggcgcc ggtagcagcg ctcgtagccc gccatgagct
     6481 cctcgccgct catgagcttc ggctcgaaca cgacgtgcgc cgtgtcgtag aggcgccagt
     6541 cccgatgcac gatgcgcttc tccttctcga gctgctcgaa cagcggcgtg ccggggtacg
     6601 gcgtgaggat gtggaacgtc gcgcactcga gccggttctg ctcgacccac tcgacggtct
     6661 tctcgaacac ggtcgcgtcg tcgtgatcga agccgagcac gaagctgccg ttcacctgaa
     6721 tgccgttgtc gtggaggatc gcgacgcggc gcgcgtagtc gtcggggacg ggcgagcgct
     6781 tgccggcgtc tcgaatgttg ccggcgttca gcgactcgaa gccgatgaag acgccggtgc
     6841 agcccgcgag cgccatctcg cgcacgagcg acgggtcgtc cgtcacgtcg atcgtcaacg
     6901 cggcgctcca gatcatgtcg agcggtctca gcgcgcggca gagctcgcgc aaatagtcgg
     6961 gcctgcttcc caaattgttg tcgacgaaca cggcgtacgg ctggccgtcg gctttgagct
     7021 cggcgacgac ttgctcgaca tcgcggagcc tgtacggcat gtgcaggcct tcggtcgaga
     7081 gatagcagaa gccgcagcgg ttatggcagc cgcgcgtcgc gatgacgctc gacgtcgtga
     7141 gaaagccgtc gcgcggcagc aggtcgcggc gcggcgccgg atcgtcgcga taaggcttgc
     7201 ggtagtcgcc gcggtagaca ggcttcaacg tgccggcctc gacgtccgcg aggatctgcg
     7261 gccacacctg cacgccttcg ccgagcgcga tcgcatccgc gtgcggcgcg cactcctcgg
     7321 gcgccgacaa cacgtgcagg ccgccgaaga cgactttcgc gccccactgc cgataccact
     7381 gcgcgagctc gtacgcgcgc ttcgcgaacg tcaggtgcac ggtgatcgcg acgacctcgg
     7441 gaaacggctc gagcggcggc gggccctgaa gcagattctc gtcccagtag ccgagctgcc
     7501 aacggtccgg cgtggcgccc gcgatcgcgg tcagcgcgag cgtcggcgtc agcacgtgct
     7561 tgccgaagct cgcgtgcggg tctttgcgat agaacgggtt cagcagcagc gcgtagcgct
     7621 tgcgccgcgg gcccgggatc gcgagcgggt cgagcgccgg ggcgacggcc atgggctcgg
     7681 gcaaccacgg ggagggggat gcggttcgag acatgacgcc gcctcggcaa ctcgatgtgc
     7741 ctatcgtcaa gtactttgca tggcaaagtc aatgacgccg aagccagcgc cgaggccttc
     7801 atgctggccg ttgcagcaag cgcgtaacgc gttacagtga aggaatgagc atggcgctcg
     7861 cagctgacag gatgagggcg atgcgtgaac ggcgccgggc tcgcggcctt cgcgagcttc
     7921 ggctcgtcgt gccggacgcg cgctcgaagg ccgtacgccg gcgagtagcg aaggctgtag
     7981 ccgccttgga tcggtcgagc gagatcgacg cgatgaagtg gattgaagcg gtctcggaat
     8041 tcgatacgcg atgaagcgcg gcgacgtcgt gaccgtggct gcgaccggcg actacggcaa
     8101 gccgcgccct gcagtgatcg tccagaccga tgccctgccg gccgagcacg cgtcggtggt
     8161 cgtttgccaa atgacttctg aattgagcgt tgccgcggac ttccgcgtca atatcgagcc
     8221 gacagagagc aacggtctgc gcgctccatt tcaggtgatg gcggacaagc cggtgacgat
     8281 tcgtcgcgag cggatcggca aacgtgtcgg ccggctcgac gaagcggaca ttgcacggct
     8341 caacgtcgcc ctcgccttcg tcatggggtt agcggactag ccccttcaag gggacagatt
     8401 tatttttccg cagaaaataa atctgtcccc ttttcggttg gtcagtcgag ctcgcgggcc
     8461 tctttgctga tcgtcgtctc gccgggcttg cgcagcatct tgtcgatctc gtcgatgtgc
     8521 gcttcctctt gcgtgatcaa gtcgcgcgcg tactcttcga gcgcgacgga gctgccttcg
     8581 acgagcttca gcagctccat gtacaggccg agcgcatagt gctcggcctg caaggtttcc
     8641 cggaggattt gcccgatgtc gtgcgcatgg gtctcgagca ggtcgccgat cgcgagcgac
     8701 ggatgcccgc cgagccgcgt gaccatctcg ccggcctggt gcgcgtgctg cagcgattcg
     8761 gccgcctcgt cttgaaacca tttgacgatc gggatccggc tgtagccgaa aatcatcatg
     8821 gaatagtgcg tgtagcgcac gacgcccgcg agctcgcatt cgaggatgcg attgagctgg
     8881 tcgatgacct tcttggtatt gagcgccatg gcggactcct ctcgttttcg atcaagcgaa
     8941 acgcggcaga aacgcgagag cgttcgcgcg ctcgacgcgc gcgagctctt gcgccgtgaa
     9001 gccggccgac tgcagccctc gcagctgcgg cgcgccgtcg aagaacggcg cgtcggtgcc
     9061 gaacacgatc tgcgacattc cgacgagcgt cttcaatgcc tgcatgttga tcgggttcgt
     9121 tgcgcccgcc gtgtcgtagt agaagcgccg caagtgatgc agcctcgtgt tcggctcggg
     9181 cgtcttcgcg agattcgcgg cgctcatctc gg
//
LOCUS       DASSNN010000507         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1601437, whole genome shotgun sequence.
ACCESSION   DASSNN010000507 DASSNN010000000
VERSION     DASSNN010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601437"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFL84_09415"
     CDS             <1..289
                     /locus_tag="VFL84_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7608882.1"
                     /translation="GIVMFLDVPGADPESVDVRMQDRLLSIVAHSTFTPPAGYSLVHC
                     EQPSGSYARQFAISEDVDEEHIAAALKDGVLRLDLPKAQKSSVKQIPVTHA"
     gene            390..824
                     /locus_tag="VFL84_09420"
     CDS             390..824
                     /locus_tag="VFL84_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7608883.1"
                     /translation="MLTLQQDINRLFDTFWRGMGMPMPSAWNELATEDLPRVDVRETD
                     KEVVVSAELPGMDEKDVEISVAGGLLSIRGETARERETENDGYVQRERTVGAVERLVP
                     LPDGLDLDSARATMKNGLLTVKIPRAGAASSDGAKQISIQRS"
     gene            1167..2441
                     /locus_tag="VFL84_09425"
     CDS             1167..2441
                     /locus_tag="VFL84_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7608884.1"
                     /translation="MPRIAAAAIAGVVDRWQTGHQVRRFDALVERSSDFIAVVTLEGL
                     LTYVNPAALRILGLESVEAARRRALLDFVPIEKRTGARTQVWPGVLRDGRWTEQLELF
                     NERTGTAVPLLVDWLRIDDAYSGTPMSLAAIGRNLVAKRLSEAKVKCLCKTLERRASA
                     RTARLTARNHDLQPELIEQRRVEARFRGLQLELFHAAHMSAAAQMAGVLAHELNQPLT
                     AATNFMNAARRFRAGARTENEGDVVSDNIAEAAKQMVRAAKILGRYRAFAACREASKR
                     VEQIPTLVAEASTVAFAGSGSLRVGFSVHCEPKAVSVVCDGIQIRQVFVNLMLNALEA
                     MGERSDGSLGVRTTLVGSDTVEIAVSDTGPGFSDAIYGQLFEPFVATTNNRVGLGLSI
                     CRKIVEAHGGRLQAETNDGGGVTVRFTLPAAL"
     gene            2441..3094
                     /locus_tag="VFL84_09430"
     CDS             2441..3094
                     /locus_tag="VFL84_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7608885.1"
                     /translation="MTSAERSVHVVDDDPDVLRSLGQVLLAAGFDPVLYETPFALLDA
                     VDRLADGCVLLDVRMPRMDGLQLQERLHELGSRSPVIFITGQGDIPMAVRAMKCGAVD
                     FIEKPFDDDRLVSSVESALALASRRSKYQGTREAAQRIATLSRRERQVLEGLMAGRTN
                     KGIARDFGLSVRTVEVHRARMLERLGTQTLAEAIRLAVTATLGPREAVAASRKRPSD"
BASE COUNT          568 a          983 c         1039 g          538 t
ORIGIN      
        1 cggaatcgtg atgttcttgg acgtcccggg cgcggatccc gagagcgtcg acgtgcggat
       61 gcaggatcgc cttctaagca tcgttgcgca cagcacgttc acgccgccgg ccggttactc
      121 gctcgtgcac tgtgagcagc cgagcggcag ctacgcgcgg caattcgcga tatcggagga
      181 cgtcgacgag gagcatatcg ccgcagccct caaggacggc gtgttgcgcc tggatcttcc
      241 gaaggcgcag aagtcgtccg tcaagcaaat ccccgtgact cacgcatagc gccgtaggag
      301 aacaggtcat gaatgcttcg agccttctgc cgtggaagcg tacccgcagc gacgccgccc
      361 gacgccgcga cggcgaccgc gagaccggcg tgcttacgct gcagcaggat atcaaccggt
      421 tgttcgacac gttctggcgg gggatgggga tgccgatgcc ttctgcctgg aacgagctcg
      481 cgacggagga cctgccgcgc gtcgacgtac gcgagacgga caaggaggtc gtcgtgtcgg
      541 ccgagctgcc gggcatggat gaaaaggacg tggagatcag tgtcgccggc ggcctgctca
      601 gcattcgcgg agagaccgcg cgcgaacgcg agaccgagaa cgacggctac gtgcaacgcg
      661 agcgcaccgt gggcgcagtc gagcgcctcg tgcccctgcc cgatggcctc gatctcgatt
      721 cggcgcgggc gacgatgaag aacggtctgt tgaccgtgaa gattccccgg gcaggggccg
      781 cgtcgtcgga cggggccaag caaatctcga tccaacgatc gtaatccctt agcgccccga
      841 tcatggccgg cgcgctcgtc gattgctgtg gcgctacacg atcgacctgt gtagtcttga
      901 cttgacataa ttgcaggtcg ccatgagatt gctttgaggc atccgaagca tcgcccgctt
      961 ttcccaacgg tagcgctcac accgcgcagc cgtggccgcg aacaggttca aagcttcgtc
     1021 gtcgctcctt aaaatttctc atgccgtggc gcgggcacat cgtcgacgct tctatttttc
     1081 gccgccgcgt ccgcgatctc gtcgccttat cgaaatcgcc ggtcccatca ccgagctcga
     1141 accttccgac acgcgcgcgt cgcgtcgtgc cccggatagc cgccgccgcg atcgcaggcg
     1201 tcgtcgaccg ctggcaaacc gggcaccagg ttcggcgctt cgacgcgctc gtcgaacgtt
     1261 cctcggactt catcgccgtg gtcacgctcg aaggcttgct cacctacgtc aacccggcag
     1321 cgctcaggat cctcggtctg gagtccgtgg aagcggcgcg acgcagagcg ctcctcgatt
     1381 tcgtgcccat agagaagcgc accggcgccc gcacgcaggt gtggccggga gtattgcgcg
     1441 acggccggtg gacggaacag ctcgagctgt tcaacgaacg caccggcacg gccgtgccat
     1501 tgctcgtcga ctggctgcga atcgacgatg cctatagcgg gacaccgatg agcctcgccg
     1561 ccatcggccg caacctcgtt gccaaaagac tctcggaagc gaaggtgaag tgcctttgca
     1621 aaacgctcga gcggcgcgca tcggcacgga cggcgcggct cacagctcgg aatcacgatc
     1681 tgcagccgga gctcatcgag cagagacgcg tcgaagcgcg cttcaggggg ctgcagctgg
     1741 agctgtttca tgcggcgcac atgagcgcgg ctgcgcagat ggccggcgtg ctcgctcatg
     1801 agctcaacca gccgctgacc gccgctacga acttcatgaa cgccgcgcgg cgctttcgcg
     1861 cgggagcacg aacggagaat gaaggcgacg tcgtctcgga caatatcgcc gaggccgcga
     1921 aacaaatggt tcgggccgcc aaaatcctgg gccgttaccg cgcgttcgct gcctgcaggg
     1981 aggcaagcaa gcgagtggag cagataccaa cgttggtggc ggaggcgagc acggtggcgt
     2041 tcgccggctc cgggtcgctt cgtgtcggct tcagcgttca ctgcgaaccg aaggccgtga
     2101 gcgtcgtctg cgacggcatt cagattcgcc aagtgttcgt caacttgatg ctcaacgcac
     2161 tcgaagcgat gggcgagcgc agcgacggca gcctcggcgt ccgtactaca ttggtcgggt
     2221 ccgacaccgt cgaaatcgca gtaagcgaca cgggacccgg attctcggac gccatctacg
     2281 gccaattgtt cgagccgttc gtcgcgacga cgaacaatcg cgtggggctc gggctttcga
     2341 tttgccgaaa gatcgtggag gcgcacggcg gccggcttca agccgagacc aacgacggcg
     2401 gcggcgtgac ggttcgcttc actcttccgg cggccctctg atgacgtcgg cggaacgctc
     2461 ggtccatgta gtcgacgacg acccggacgt cctccggtcg ctcggccaag tgttactggc
     2521 ggctgggttc gacccggtcc tatatgaaac gccgttcgcg ttgctcgacg ccgtcgatcg
     2581 gctggcggat gggtgcgtcc tgctcgatgt gcgcatgccg agaatggatg ggttgcagct
     2641 ccaggagcgc ctgcacgagc tcggaagtcg ctcgccggtg atcttcatca ccggccaggg
     2701 ggatataccg atggccgtcc gcgcgatgaa gtgcggcgca gtcgatttca tcgagaagcc
     2761 gttcgacgac gatcgattgg tctcttcggt cgagtcggct ctcgcgctcg catcgaggcg
     2821 cagcaagtac caagggacgc gcgaggccgc tcagcggatc gcgaccttga gccggcgaga
     2881 gcgacaggtt ctagaggggc tcatggccgg gcggacgaac aagggcatcg cgcgggattt
     2941 cggcttgagc gtacgcacgg tcgaagttca tcgtgcacga atgctggaac gcctcgggac
     3001 gcagaccctt gccgaagcca tccgactcgc ggtaaccgcg acgctggggc cgcgcgaggc
     3061 agttgcggcg agccgcaagc ggccttctga ctaagctagt gagcataccg agggcgcggc
     3121 ttcacgcg
//
LOCUS       DASSNN010000508         7064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_592302, whole genome shotgun sequence.
ACCESSION   DASSNN010000508 DASSNN010000000
VERSION     DASSNN010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592302"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1043
                     /locus_tag="VFL84_09435"
     CDS             15..1043
                     /locus_tag="VFL84_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HET7608886.1"
                     /translation="MKRRTWVALAVVTGSILIVGAFLHLHSEALHGHFSALHHGAGMH
                     ATGPAAELLGGAGGDPAVNARAREGVAEIGRRWDEDAFARTVALYTEVHRGIDWPGVL
                     APETFQYGSDARQTLELYRPEQEFGEPGPVFLFLHGNGLGNADRVVPGSDGLIYSHLG
                     KLAATAGGIGVSMGYRIGAGKVGVAALESGAEDLRLVIEWIATKIGAYGGDPNTIVIV
                     ANSEGATVAAGYLFNEQWQTKAGHGVAAAILSSGMFGALAPEIEPLIFNYAGKPVPLA
                     LWSAEYDVAGVTAGIADLHEILCRKYYGCPWYEQINGHNHVSQLMSLGTADTDVMNRF
                     IRFYHTVR"
     gene            complement(1015..1911)
                     /gene="gluQRS"
                     /locus_tag="VFL84_09440"
     CDS             complement(1015..1911)
                     /gene="gluQRS"
                     /locus_tag="VFL84_09440"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HET7608887.1"
                     /translation="MRYVGRYAPSPTGPLHLGSLVTAVASFLHARQAGGEWLVRIEDI
                     DPPREVPGAADDIQRTLEAFELEWDRSVLYQSTRLAAYEVAARRLLADGRAFHCRCSR
                     SEIRAANEGESGRYPGTCRDLRIPPGDAAVRVRVDSGSVAFDDALQGTIETDLATTLG
                     DYVIVRRDGLPAYHLAAVLDDAEQGVTTIVRGVDLLDSTAVHVHLQRVLALATPQYYH
                     VPVVVNEQGQKLSKQTGARAVDAHDRAAAARVLELLGLAVPSALAGERPRVLWQWAIE
                     RWHIDSLRGQRSFASARCDRSG"
     gene            complement(1928..2422)
                     /gene="dksA"
                     /locus_tag="VFL84_09445"
     CDS             complement(1928..2422)
                     /gene="dksA"
                     /locus_tag="VFL84_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HET7608888.1"
                     /translation="MASKPIAAPVPTRRRGGNLLTGPVKGFTPYQPKRGEEYMNKDQL
                     EHFRQLLLAWKRELMEEVDRTMLHMKDDAANFPDPNDRATQESEFGLELRTRDRERKL
                     LKKIDSALARIDDGSYGYCEETGDEIGLRRLEARPVATLCVEAQERRELAERQFRDRE
                     DQYR"
     gene            complement(2775..3191)
                     /gene="queF"
                     /locus_tag="VFL84_09450"
     CDS             complement(2775..3191)
                     /gene="queF"
                     /locus_tag="VFL84_09450"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HET7608889.1"
                     /translation="MASKPAARPSKKSLETFPNPSADRDYTIRMRIPEFTCLCPKTGQ
                     PDFATLHLEYVPDSKCVELKSLKLYIWSYRDEGAFHEAVTNRILDDLVQTMEPRFIRL
                     TAEFNVRGGIYTTVVAEHRKKGWEPAPAVALPADSR"
     gene            3324..6827
                     /gene="smc"
                     /locus_tag="VFL84_09455"
     CDS             3324..6827
                     /gene="smc"
                     /locus_tag="VFL84_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET7608890.1"
                     /translation="MRLSKIKLSGFKSFVDTTTIKFPSNLMGIVGPNGCGKSNIIDAI
                     RWVLGEASAKTLRSDSMVDVIFNGSANRKPVGLASIELTFDNSDGTITGAFAGYNEVA
                     VRRVVSRDGTSQYYLNNTRCRRKDITHILLGTGVGSHGYAIIEQGMISRLVEAKPEEL
                     RAFLEEAAGISKYKERRRETEQRIHHTRENLERLKDLREEIEKQLTHLQKQAKDAERY
                     KNLKTEQRKVNAELLALRLKALRAEVAQHEHELREKQIGLEAAIAAQTSAETSIEKLR
                     VELAERNERFSVVQGAYYKVGSEIARLEQSLQHRKELIQRQSEDLESTNSQIAELNAH
                     IDSDRVELEELERVLGELGPGLEEAHSVQRASQQALERAEQATEQARTRWEQLSEELA
                     AAERSMHVEDTRLEQLAAQSQRLEKELAKNQDERQALSFGDVEQKLESLVASEERLAA
                     ACESATRALSGVWTQIQQLREQEDRVSAELDQLRESLQTDRGRLTSLEALQEAALGKS
                     TEQLNRWLATQTLDERRRLAQDLVVENGWERAVETVLGPYLQAVGIDSIDRVAGSLAD
                     LTEGGLTLVETRGAAQARLPADALLKHMKTPAAVASLLVGVRAAASTKDAIARRHELA
                     DGESFITRDGVWVGRHWLRMNRSDDPQVGVIARGEEIKRLRETTQATSRRVEEVAKAL
                     ADTRSQRERLEEERARAQVEATQRQEQLAEAKAQLGASRAELDQARQRASALDRTVAD
                     LAAERQALVTAVDESKARRAAAVARHSELTAMRESVERERHELAERLAEARATAVQHR
                     EEAQAIAIKVETRRSSKESASAALLRVQSQLAHLSKRQQELKSAVEAAAEPMRADEQT
                     LVAKLDERLVVEADLGKARQAMDEIDTQLRETESQRNDRQRAVNEIREIADTVRLSVR
                     ESQVRAETVAEQFNATGFVLDEITLGLPGDATVEVWTETFESLERRITRLGNINLAAI
                     DEFKENSERKTYLDAQFTDLTEALETLENAIRKIDKETRARFKETFDKANDGLGRLFP
                     RLFGGGQAYLEMDGEDILAAGVTIMARPPGKKISTIHLLSGGEKALTAVALVFSIFEL
                     NPAPFCLLDEVDAPLDDANVGRFSEIVKEMSERVQFVIITHNKTTMETMHQLSGVTMQ
                     EPGVSRMVAVDIDEAVRLAAM"
BASE COUNT         1277 a         2272 c         2382 g         1133 t
ORIGIN      
        1 ggcggagaac aacgatgaaa cgtcgcacat gggtagctct ggctgtggtg accggctcga
       61 tcctgatcgt cggagccttc ttgcacctgc attcggaggc tttacacggg cacttctcgg
      121 cgttgcacca cggcgctgga atgcacgcga ccggtccggc cgcggagctg ctcggtggag
      181 cgggaggcga tcctgcggtc aacgcgcggg cgcgcgaagg cgtcgccgag attggcagac
      241 ggtgggatga agatgcgttc gcgcgaacgg ttgcgctcta taccgaagtg cataggggca
      301 tcgactggcc cggcgtgctc gcaccggaga cgttccaata cggatccgac gcgcggcaga
      361 cgctcgagct ttaccgcccg gagcaagaat tcggcgagcc cggtcccgtg tttctgtttc
      421 tgcacggcaa cggcctcggc aacgccgatc gcgtcgtgcc gggcagcgac ggcctgatct
      481 acagccatct cggaaagctc gcggcgactg ccggcggcat cggtgtctcg atgggctatc
      541 gcatcggcgc aggcaaggtc ggcgtcgcgg cgctcgagtc gggcgccgag gatctgcgtc
      601 tcgtcatcga gtggatcgcg acgaaaatcg gggcctacgg cggcgatccc aatacgatcg
      661 tgatcgtcgc gaattccgaa ggcgcgaccg tcgcggctgg ctatctcttc aacgagcagt
      721 ggcagacgaa ggccggtcac ggtgtagcgg ccgcgatctt gagctcaggg atgttcggag
      781 cactcgcgcc cgagatcgag ccgctgatct tcaactacgc gggcaagcct gtgccgctcg
      841 ctctgtggag cgcggaatac gacgtcgccg gagtcacggc cggcatcgcg gaccttcacg
      901 aaattctttg ccgcaagtac tacggctgtc cgtggtacga gcagatcaac ggacacaatc
      961 acgtctctca gctcatgagc ctcggcacgg ccgataccga cgtgatgaac cgcttcatcc
     1021 gcttctatca caccgtgcgc tagcgaacga tcgctgacca cgtagcgaat cgatgtgcca
     1081 gcgctcgatt gcccactgcc acagcacgcg cgggcgttcg cccgcgagcg cgctcggcac
     1141 cgctaggcca agcagctcga gcacgcgtgc agccgccgcg cggtcatgcg cgtcgactgc
     1201 acgcgcgccg gtctgcttcg acagcttctg gccttgctcg ttgacgacga cgggcacgtg
     1261 gtagtactgc ggcgtcgcga gcgcgaggac gcgctgaagg tggacgtgca ctgcggtcga
     1321 gtcgagcaga tcgacgcctc gcacgatcgt cgtgacgcct tgctctgcgt cgtcgagcac
     1381 ggcggccagg tggtaggcag gtaagccgtc gcgccgcacg atgacgtagt cgccgagcgt
     1441 cgtcgcgagg tccgtctcga tcgtgccctg caaggcgtcg tcgaaggcga cgcttcccga
     1501 atcgacccgc acgcgcacgg cggcatcacc aggcggaatc cgaaggtcgc ggcacgtgcc
     1561 gggatagcgg cccgactctc cttcgttcgc tgcgcggatc tcgctgcgcg aacagcggca
     1621 atgaaaagcg cggccgtcgg cgagcaagcg ccgggcagcc acctcgtagg ccgcgagcct
     1681 ggtgctctga tagaggacgg atcgatccca ctcgagctcg aacgcctcga gcgtgcgctg
     1741 gatgtcgtcg gcggcgccgg gcacctcgcg cggcgggtcg atgtcctcga ttcgcacgag
     1801 ccactcgccg cccgcttggc gcgcgtgcag gaagctcgcg acagccgtta cgagcgaccc
     1861 caaatgaagg gggcccgtag gagaaggagc gtagcggccg acgtagcgca cgctcggccg
     1921 cccaacctca gcggtactga tcctcgcggt cgcggaactg ccgctcggcg agctcgcggc
     1981 gttcctgcgc ttcgacgcag agcgtcgcga cgggccgcgc ctcgaggcgg cgcagtccga
     2041 tctcgtcgcc ggtctcctcg cagtagccgt acgagccgtc gtcgatgcgc gccaatgccg
     2101 agtcgatctt tttcaaaagc ttgcgctccc tatctcgagt gcgcagctcg agaccgaact
     2161 cggactcctg cgtcgcgcgg tcgttcggat cggggaagtt cgccgcatcg tccttcatgt
     2221 gcagcatcgt gcggtcgact tcttccatga gctcgcgctt ccacgccagc agcaactggc
     2281 gaaagtgctc gagctgatcc ttgttcatgt attcctcgcc gcgcttcggc tgatagggcg
     2341 tgaagccctt gaccggaccg gtgaggaggt tgccgccgcg ccgacgcgtc gggacgggcg
     2401 cagcgatcgg tttcgacgcc aaacgaatcg gcgccggcgg gccggagccc ggctttttcg
     2461 gtatcgtggc gatcgtcttc ttccctgatg gttttggttg ttccttgggt acagccagct
     2521 tgggcgagac cttcttgacg ctcgttcgtt tcgctgactt cccggttttt gcagaggatt
     2581 tcggcacggc cctgagcttg gccttgctgg gtcggctggc cgcagaagaa gctttcttgg
     2641 ccggcttttt cttgccggcg ggcgcctttt tcctggctgc tgcttttttc gcggacatgg
     2701 ccttggcctc caagaccctt caaaaggagc cggcgattat acagacgccc cggggtagcg
     2761 gaagagggca gacgtcaccg cgaatcggcc ggcagcgcga ccgccggagc gggctcccag
     2821 ccctttttgc ggtgctcggc gacgaccgtg gtgtagatgc ccccgcgcac gttgaattcc
     2881 gccgtcaaac gaatgaatcg cggctccatc gtctgaacga ggtcgtcgag gatgcggttc
     2941 gtgaccgctt cgtgaaatgc gccttcgtcg cggtaagacc aaatgtagag tttcagcgat
     3001 ttcagctcga cacacttcga atctggaacg tactcgaggt gcagcgtcgc gaagtcgggc
     3061 tggcccgtct tcggacagag acaggtgaac tccggaatgc gcatccggat cgtgtagtcg
     3121 cgatccgcgc tcgggttcgg aaacgtctcg agactctttt tcgacggccg agcagcaggc
     3181 ttactcgcca agttttcgtg ttccgattga aggtgaaaag cgacaggcgc ggccgtcgct
     3241 tttggattgg tttttactct acactaccgc cgtcgcagcg gccatgaggg cctctcaaag
     3301 ctcgaccacc gcggggtcaa acattgcgcc tgagtaagat caagctttcg ggcttcaagt
     3361 cttttgtcga tacaaccaca ataaaatttc cgagcaactt gatgggcatc gtcgggccca
     3421 acggttgcgg caagtccaac atcatcgacg cgattcgctg ggtgctcggc gaggcgtccg
     3481 cgaagacgct gcgcagcgat tcgatggtgg acgtcatctt caacggctcc gcgaaccgca
     3541 agcccgtcgg gctcgcgagc atcgagctca cgttcgacaa ctccgacggc acgatcacgg
     3601 gcgcgttcgc gggctacaac gaggtggctg tccgccgcgt cgtctcgcgc gacgggactt
     3661 cgcagtacta cctcaataac acgcgctgcc ggcgcaagga catcacgcac atcctgctcg
     3721 gcacgggcgt cggctcgcac ggctacgcga tcatcgagca gggcatgatc tcgcggctcg
     3781 tcgaggccaa gcccgaagag ctgcgcgcgt tcctcgagga agcggccggc atctccaaat
     3841 ataaggagcg ccggcgcgag accgagcagc ggatccacca cacgcgcgag aacctcgagc
     3901 gcttgaagga tctgcgcgag gaaatcgaga agcagctcac gcacctgcaa aagcaggcga
     3961 aggacgcgga gcgctacaag aacttgaaga ccgagcagcg caaggtcaac gccgagctgc
     4021 tcgccttgcg cttgaaggcg ctgcgcgccg aggtcgcgca gcacgagcac gagctgcgcg
     4081 agaagcaaat cggtctcgag gccgccatcg ccgcgcaaac gtccgcggaa acctcgatcg
     4141 agaagctgcg cgtcgagctc gcggagcgca acgagcgctt cagcgtcgtt caaggcgcgt
     4201 actacaaggt cggctccgag atcgctcggc tcgagcagtc gctgcagcat cgcaaggagc
     4261 tgatccagcg gcagagcgag gatctcgaga gcaccaacag ccaaatcgcc gagctgaacg
     4321 cccacatcga cagtgacagg gtcgagctcg aggagctcga gcgcgtgctc ggcgagctcg
     4381 gccccgggct cgaagaggcg cactcggtcc agcgtgcttc gcaacaggcg ctcgagcgcg
     4441 ccgagcaggc cacggagcag gcgcgcacgc gctgggagca gctcagcgag gagctcgcgg
     4501 cggccgagcg ctcgatgcac gtcgaggaca cgcggctcga gcagctcgct gcgcagagcc
     4561 agcggctcga gaaggagctc gcgaagaacc aggacgagcg gcaagccctg agcttcggcg
     4621 acgtcgagca gaagctcgaa tcgctcgtcg cgagcgagga gcggctcgcc gccgcctgcg
     4681 agagcgcgac gcgcgcgctg agcggcgtgt ggacgcagat tcaacagctg cgcgagcaag
     4741 aggaccgcgt atcggcggag ctcgaccagc ttcgcgaaag cctgcaaacg gatcgcggcc
     4801 ggctcacgtc gctcgaggcg ttgcaggaag ccgcgctcgg caagtccacg gagcagctga
     4861 accgctggct cgcgacgcag acgctcgacg agcggcgccg gctggcgcaa gacctcgtcg
     4921 tcgaaaacgg ctgggagcga gccgtcgaga ccgtgctcgg cccgtatttg caggccgtcg
     4981 gcatcgacag catcgatcgt gtcgcgggct cgctggccga cctcacggaa ggcggcttga
     5041 cgctcgtcga gacccgaggc gccgcccagg cgcggctgcc cgcggatgcg ttgctgaagc
     5101 acatgaagac gccggccgcc gtggcgtcgt tgctcgtggg cgtccgcgcc gccgcttcga
     5161 cgaaggacgc gattgcgcgc cgccacgagc tcgcggacgg cgagtccttc atcacgcgcg
     5221 acggcgtctg ggtcgggcgt cactggctgc gcatgaatcg cagcgacgat ccgcaggtcg
     5281 gcgtcatcgc tcgcggcgag gagatcaagc gtcttcgcga gaccacgcag gcgacttccc
     5341 gccgcgtcga agaggtcgcc aaggcattgg ccgatacgcg ctcgcaacgc gaacggctcg
     5401 aggaagagcg cgcgcgagca caggtggaag cgacgcagcg gcaagagcag ctcgcggaag
     5461 cgaaggcgca gctcggcgcg agccgcgcgg agctcgacca agcgcggcaa cgcgcttcgg
     5521 cgctcgatcg caccgtcgcg gatctcgcgg ccgagcggca ggcgctcgtc acggccgtcg
     5581 acgagagcaa ggcgcggcgt gcggccgccg tcgctcgtca cagcgagctc acggccatgc
     5641 gcgagagcgt cgagcgtgag cggcacgagc ttgccgagcg cctggccgaa gctcgcgcga
     5701 cggcggtaca gcatcgcgag gaagctcagg ccatcgcgat caaagtcgag acgcggcgca
     5761 gctcgaagga gtccgcgagc gcggcgctgc ttcgggtgca gagccagctc gcgcacctgt
     5821 cgaagcgcca gcaggagctc aagagcgcgg tcgaagccgc ggccgagccg atgcgcgccg
     5881 acgagcagac cttggtcgcg aagctcgacg agcgcctcgt ggtcgaggcc gatctcggca
     5941 aggcgcgcca agcgatggac gagatcgaca cgcagctgcg cgaaaccgaa tcgcagcgca
     6001 acgaccgtca gcgcgccgtc aacgagattc gcgagatcgc cgataccgtc cgtctctcgg
     6061 ttcgcgagtc gcaagtgcga gccgagaccg tcgccgagca gttcaacgcg acggggttcg
     6121 tgctcgacga gattacgctc ggccttcccg gcgacgcgac ggtcgaggtt tggaccgaga
     6181 cgttcgagtc gctcgagcgg cgcatcacgc ggctcggcaa catcaatctc gccgcgatcg
     6241 acgagttcaa ggagaactcg gagcgcaaga cctatctcga cgcgcagttc acggatctca
     6301 ccgaggcgct cgagacgctc gagaacgcga tccgcaagat cgacaaggag acgcgcgcgc
     6361 gcttcaagga gacgttcgac aaagcgaacg acggcctcgg ccgcctgttc ccgcggctct
     6421 ttggcggcgg ccaggcgtat ctcgagatgg acggcgagga cattctcgcg gccggcgtca
     6481 cgatcatggc tcggccgccc ggcaagaaga tcagcacgat tcacttgttg tcgggcggcg
     6541 agaaagcgct gacggccgtc gcgctcgtct tctcgatctt cgagctgaat cccgcgccgt
     6601 tctgcctcct cgacgaggtc gacgcgccgc tcgacgacgc gaacgtcggg cgcttctcgg
     6661 agatcgtcaa ggaaatgtcc gagcgcgtgc agttcgtcat catcacgcac aacaagacga
     6721 cgatggagac catgcatcag ctctccggag tgacgatgca ggagcccggc gtgtcgcgca
     6781 tggtggcggt cgatatcgac gaagcggttc ggctcgcagc aatgtagtcg cggctcgcca
     6841 tgtgggactt acgctgggta ctgttggggc tgggtgccct cgtactaatc gcggtatacc
     6901 tatggagccg cggaatcgtg tcgcgcgaca tgcttccgac gctgccgcgc cgcaaagagc
     6961 gcaccgagcc cagcatcggc gagccgccgc ctacacctga gcccacaccg cagcccgagt
     7021 cgctcgtcga gaccgtttcc gaggaagaga aggcgcgccc cggg
//
LOCUS       DASSNN010000509         4238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_829574, whole genome shotgun sequence.
ACCESSION   DASSNN010000509 DASSNN010000000
VERSION     DASSNN010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829574"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFL84_09460"
     CDS             <1..482
                     /locus_tag="VFL84_09460"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET7608891.1"
                     /translation="GRWPNVTADLLVDDVIFPVCRADVARRLKRPSDLLGEQLLTETP
                     SWDLWTDWFAAAGVPHSPPKPFRLSDDFYVQLQATLLGRGVTLARGLLVADALREGRV
                     VCPFPIAAPSRLQYYVVCSPERHDERAIVMLRGWLIEAARTTVADLPALIERGVRAR"
     gene            610..1644
                     /locus_tag="VFL84_09465"
     CDS             610..1644
                     /locus_tag="VFL84_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7608892.1"
                     /translation="MSAHDTLSDVLRTVRLRTAVFYYVSCDGEWAAEAPCSRDIAAAV
                     MPEAEHVMEYHVLTSGECWAGLVGQEPIKMRRGDVVLLPHGDAHVVSSAPGMRADPAV
                     DGYFQRAPARRPFRFHYDGAAPATLLAVDGQPPPPKVPATAATFVCGFIGCDLKPFNP
                     LIATLPRLLHLPAEGRSALSEQFATFAAAESAARRPGSEALLERLSEMMFVDAIRRHV
                     EQLPPDTTGWLAGLRDRYVGRALALLHERPAAPWTIEDLSSQVGLSRSALHERFVSLI
                     GQPPVQYLTSWRMQLASRLLLEGRATVATVAVDVGYDSEAAFARAFKRLVGMPPAAWR
                     RAHEPAAAGA"
     gene            1647..3401
                     /locus_tag="VFL84_09470"
     CDS             1647..3401
                     /locus_tag="VFL84_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7608893.1"
                     /translation="MRGAIAVHLAAACAFVSAAAAAAERVTLSIVGTNDLHGRFMTDE
                     RGRGGLAVLGGYVDNLRAARAADGGGVLVLDAGDTFQGGIESNLAEGLMIVDAYNAIG
                     YTALAVGNHDFDYGPVDPREPAALWLPRHVRGEPIGDVQGALKAAAARAHFPFLAANI
                     VDAASGKPVSWPNVAPSALVEVAGVRVGLVGTITETGLKQTLAAHVVGLETRPLAPTI
                     AAEARALRERGADIVVLATHAGGWCGETADPHDLASCDDGSEIFKLARELPRGTLQAI
                     VAGHTHGTVAHFVNGVPIISVPNFGGQFGRVDLTVDTARREVASVRIHEPQAVCTRVE
                     PRSGECAAAPGGVAAEYEGRPVIASAAVAAAIEPERKRVLALRAEPLGAVADAPLARG
                     AGLDGTLGNLFAEALLAVVPDADVALGYGAGRGGLRADLAEGPVTFGQAYDVFPFDNR
                     ITRVALTGAQLTRVIAAQLPLWIDGRRGLPGLAGLRVTIDCSGAQAEVRLTRRMGGVV
                     GADDPLVLAMASNTVGRFAATALPGEPEIVTAELPILVRDAFADWLLERGGHLAPAEV
                     AGRWHLPPQGIRCGAPPE"
     gene            3556..>4238
                     /locus_tag="VFL84_09475"
     CDS             3556..>4238
                     /locus_tag="VFL84_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608894.1"
                     /translation="MDKYCRLWTCALAALALHAFDAHGQRGDDDEGPRGGVICFGALG
                     DTRVEGDLNVVGRCTLNGTEVRGNVTLFVAGALVARDARIRGDIEGSRANFVDLQRTR
                     IDGKVELDELVGDLTTFVESELHGDVELTSNRSALEILNSEFSRNLVVSRNTGGVTIS
                     GNFVGNDLRCDANDPAPTGTANTIEGENEGQCASLVPTDPPPQPAPSPPPPSPPAPAP
                     APAPAPAPAP"
BASE COUNT          573 a         1524 c         1517 g          624 t
ORIGIN      
        1 aaggccgctg gcccaacgtg acggccgacc tgctcgtcga cgacgtcatc tttccggtct
       61 gccgcgccga cgtcgcgcgg cgcttgaagc ggccgagcga tctgctcggc gagcagctgc
      121 tcacggaaac cccaagctgg gacctgtgga ccgactggtt cgccgcggcg ggcgtcccgc
      181 attcgccgcc caaacccttt cgcttgagcg acgacttcta cgtgcagctg caggcgacgt
      241 tgctcggccg cggcgtcacg ctcgcgcgcg ggttgctggt cgccgacgcg ctgcgcgagg
      301 gccgcgtcgt ttgcccgttt ccgatcgccg cgccgtcacg gctgcagtac tacgtcgtct
      361 gcagcccgga gcggcacgac gagcgcgcga tcgtcatgct gcgcggctgg ctgatcgagg
      421 cggcgcgaac gaccgtcgcc gacctgccgg cgctgatcga gcgcggcgtg cgcgcccgtt
      481 gagcagggcg ggacgccggg ttagcttggg agcacggcgg atcgaatgag gagcatcgct
      541 tgccaggcct ttcgcgccgc aggttgccat cgctgacgta cagcctcgag ctgccgacgg
      601 agtgacgagg tgagcgcgca cgacacactg tccgacgtgc tgcgcacggt ccggctgcgc
      661 acggcggtgt tctactacgt ctcgtgcgac ggcgaatggg ccgccgaggc gccttgctcg
      721 cgcgacatcg cggcggccgt catgccggaa gccgagcacg tcatggagta tcacgtgctg
      781 acgtccggcg aatgctgggc cggtctcgtc gggcaagagc cgatcaagat gcgccgcggc
      841 gacgtcgtgc tgctgccgca cggcgacgcg cacgtcgtct cgagcgctcc cggcatgcgc
      901 gccgatcccg cggtcgacgg ttatttccag cgggcgccgg cgcggcggcc gtttcgcttc
      961 cactacgacg gcgcggcgcc ggctacgctc cttgcggtcg atggccaacc accgccgccg
     1021 aaagtgccgg ccaccgcggc cacgttcgtc tgcgggttca tcggctgcga cctgaagccg
     1081 ttcaatccgt tgatcgcaac gctgccgcga ttgctgcacc tacccgccga gggccgcagc
     1141 gcgctcagcg agcagttcgc gacgttcgcg gcggcggaat ccgcggctcg ccgccccggc
     1201 agcgaagcgc tgctcgagcg cttgagcgaa atgatgttcg tcgacgcgat tcgccgccat
     1261 gtcgagcaat tgccgcccga cacgacgggc tggctcgccg gcttgcgcga ccgttacgtc
     1321 ggccgcgcgc tcgcgctgct gcacgaacgg cccgcggcgc cgtggacgat cgaggacttg
     1381 agcagccagg taggcctgtc gcgctcggcg ctgcacgagc ggttcgtgtc gctgatcggc
     1441 cagccgcccg tgcagtacct gacgagctgg cgtatgcagc tcgcctcgcg cctgcttctc
     1501 gaagggcgcg cgacggtcgc gacggtcgcc gtcgacgtcg gctacgactc cgaggcggca
     1561 ttcgcacgcg cgttcaagcg gctcgtcggc atgccgccgg cggcgtggcg ccgcgcgcat
     1621 gagcccgcag cggcgggggc ctgagcatgc ggggcgcgat cgcggtgcat ctcgcggcgg
     1681 catgcgcttt cgtgtcggcg gccgcggccg ccgccgagcg cgtgacgctc tcgatcgtcg
     1741 gcacgaacga tctgcacggc cggttcatga ccgacgagcg gggccgcggc gggctcgcgg
     1801 tgctcggcgg ttacgtcgac aacctgcgcg ccgcgcgagc ggcggacggc ggcggcgtgc
     1861 tcgtgctcga tgccggcgac acgttccagg gcggcatcga gtcgaatctc gccgaagggc
     1921 tcatgatcgt cgacgcgtac aacgcgatcg gctacacggc tctcgcggtc ggcaatcacg
     1981 acttcgacta cggccccgtc gatccgcgcg agcccgcggc cctgtggctg ccgcggcacg
     2041 ttcgcggcga gccgatcggc gacgtgcaag gcgcgctcaa agccgccgcg gcgcgcgcgc
     2101 actttccgtt cctcgcggca aacatcgtcg acgcggcttc gggcaagccc gtgagctggc
     2161 cgaacgtcgc gccgtccgcg ctcgtcgagg tcgcgggcgt ccgcgtcggc ctcgtcggca
     2221 cgatcacgga gacgggactc aagcagacgc tcgcggcgca cgtcgtgggt ctcgagacgc
     2281 ggccgctcgc gccgacgatc gccgccgaag cgcgcgcgtt gcgcgagcgc ggcgccgaca
     2341 tcgtcgtgct cgcgacgcac gcgggcgggt ggtgcggcga gacggcggat ccgcacgacc
     2401 tcgcgtcgtg cgacgacggc agcgagatct tcaagctcgc gagagagctg ccgcgcggca
     2461 cgctgcaggc gatcgtcgct gggcacacgc acggcaccgt cgcgcacttc gtaaacggcg
     2521 tgccgatcat ctcggtgccg aatttcggcg ggcagttcgg ccgcgtggat cttacggtcg
     2581 acacggcgcg ccgcgaggtc gctagcgtgc gcatccacga gccgcaagcc gtctgcacgc
     2641 gcgtcgagcc gcgcagcggc gagtgcgccg cggcgcccgg cggcgttgcc gccgagtacg
     2701 aagggaggcc cgtgatcgcg agcgccgccg tcgccgccgc gatcgagccc gagcggaagc
     2761 gcgtgcttgc actgcgcgcc gagccgctcg gcgccgtggc ggacgcgccg ctcgcgcgcg
     2821 gcgccggcct cgacggcacg ctcggcaacc tgttcgccga ggcgctgctc gcggtcgtgc
     2881 cggacgccga cgtcgcgctc ggttacggcg cgggccgcgg cggcctgcgc gccgacttgg
     2941 cggaaggccc cgtgacgttc ggccaggcct acgacgtgtt cccgttcgac aaccgcatca
     3001 cgcgcgtggc gctgaccggc gcccagctca cgcgcgtgat cgccgcgcag ctgccgcttt
     3061 ggatcgatgg gcgccgcgga ttgcccggtc tcgccggcct gcgcgtgacg atcgactgca
     3121 gcggcgcgca ggccgaggtg cgcctcacgc gacgaatggg cggcgtcgtc ggcgccgacg
     3181 acccgctcgt cctggccatg gcctcgaaca ccgttggccg gttcgcggcc acggctctgc
     3241 ccggcgagcc cgagatcgtg actgccgagc tgccgatcct cgtccgcgac gcgttcgccg
     3301 actggctgct cgagcgcggc ggccacttgg ctccggccga ggtcgcgggg cgctggcacc
     3361 tgccgccgca aggcattcgc tgcggtgcgc cgcccgagta gaccgcaccc accggacgct
     3421 atccagaatc tgatttgatt ttgcgagaag cgcggcctcg cccactttcc gggacggctc
     3481 gaagcgccca ctcgcgccgc tcgagccggc ccggcttttg catttagagg cagaaatggg
     3541 acgagggacg accccatgga taagtattgc cggctatgga cgtgcgcgct cgccgcgctg
     3601 gctttgcacg ctttcgacgc tcacgggcaa cgcggcgacg acgacgaggg cccgcgaggc
     3661 ggcgtcattt gtttcggcgc gctcggcgac acgcgcgtcg agggcgatct gaacgtcgtc
     3721 ggcaggtgca cgctgaacgg caccgaggtc cgcggcaacg tcacgctgtt cgtggccggc
     3781 gctttggtcg cgcgcgatgc gcgcatccgc ggcgacatcg aaggcagccg cgcgaatttc
     3841 gtcgacctgc aacgcacgcg catcgacggc aaggtcgagc tcgacgagct cgtcggcgac
     3901 ttgacgacgt tcgtggagag cgagctgcac ggcgacgtcg agctcacgtc caaccgttcg
     3961 gcgctcgaga tcctgaacag cgagttcagc cgcaatctcg tggtatcgcg caacacgggt
     4021 ggcgtcacga tctcgggcaa cttcgtaggc aacgacttgc gttgcgacgc gaacgacccg
     4081 gcgccgaccg gcaccgccaa tacgatcgaa ggcgagaacg aaggccaatg cgcgagcctc
     4141 gtgccgaccg acccgcctcc gcagccggcg ccttcgccgc cgcctccttc accgccggcc
     4201 cccgcgcccg ctccggctcc ggcgccggct cccgcgcc
//
LOCUS       DASSNN010000510         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1100961, whole genome shotgun sequence.
ACCESSION   DASSNN010000510 DASSNN010000000
VERSION     DASSNN010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100961"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..410
                     /locus_tag="VFL84_09480"
     CDS             189..410
                     /locus_tag="VFL84_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608895.1"
                     /translation="MSIGDADFLGEIVGGGNYEQLLPHSIELLVFGKTIRVLDLPALI
                     RAKRAAGRPKDLEAVAELEALLVEKEREG"
     gene            483..1262
                     /locus_tag="VFL84_09485"
     CDS             483..1262
                     /locus_tag="VFL84_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608896.1"
                     /translation="MGSGEIIEEALRIARTHKSLWLFGFFVGLGGSNFGIGGVVGIIV
                     ATVAFVVLRFLGTSALIEGVTRARRNGRMTVREGFREGWANWGVLFLITLVFLPMNLA
                     TVALLGGACYLVWTVFGSPAAILAACAALLLGVPCLLTLYMWQMFAERIAVLENRRAL
                     DAMRKARLFLHGRLQLGLKLFVADLLGGLFVTVVGLIVIVPIAFVFVSARVWAASEWG
                     ALAAVGLGVLTVAPLLFVAFAFFGIMTTSVWTIGYLTQVEQ"
     gene            1265..1927
                     /locus_tag="VFL84_09490"
     CDS             1265..1927
                     /locus_tag="VFL84_09490"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7608897.1"
                     /translation="MTATMAALPSAPFFLRIYNVLSVLFGGDAEPLEEDAELRLVELA
                     RAGDPVARQRLYRRHVQRVFRTVRGMLRSDAEAEDVTQDAMLSAMTSLDRYSPRSGTR
                     FVAWLTTIAVNTTRRRFRRRRPELTATGELPDVVDADSDLASDLDLARQRGALMQALA
                     ELEPRERAIVSLRYGSDLNATEIAAQMGMEAANVRKILERVRARLGERIEALAKSRGA
                     AA"
     gene            1924..2163
                     /locus_tag="VFL84_09495"
     CDS             1924..2163
                     /locus_tag="VFL84_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608898.1"
                     /translation="MNDAEIMNLWCALEPSSRRRARIETRVFEWLEASETLVAAEWLR
                     LLKVKPLTTLTYAAVAALALLALTPVGWIAAWLLP"
     gene            complement(2213..2431)
                     /locus_tag="VFL84_09500"
     CDS             complement(2213..2431)
                     /locus_tag="VFL84_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608899.1"
                     /translation="MPACSENPAYCATPRLGSSARAGTLCNENTLLDERGVKNRYLWI
                     DPRSDGAQALAAKLGCSFVFDSLDIDCA"
BASE COUNT          385 a          785 c          828 g          482 t
ORIGIN      
        1 cacaggctca tggctccgcc cgcctaacgc aggatgccga tttcgtctat ggccgcagcg
       61 acgcgaacat cgctcggctt gccgacgcac ttgctccgtt ctcggcgtac ttgcgcgggg
      121 cgccgccagg tctcccattt cggttcgatt caacgacgat tcgtcgagga cttaacttca
      181 ctctgacgat gtccatcggt gacgcggact tcttagggga gatcgtcggc ggtggaaact
      241 acgagcagct cttgcctcac agcatcgagc tgctcgtctt cggcaagacg attcgcgttc
      301 tcgacttacc cgcgctcatc cgcgcaaaac gggcagccgg gcggccgaag gacctcgagg
      361 ccgtcgcgga gctcgaagca ctactcgtcg aaaaagagcg cgaaggatag cgcgtcacac
      421 ttgtgcacct aaccggctta ttgctctgaa ggccgcgttg gcccgagccc cggagcaagg
      481 ttgtgggtag tggcgagatc atcgaagaag cgctgcggat cgcccgcacg cacaagtcgt
      541 tgtggctgtt cgggttcttc gtcgggctag gcggctccaa tttcgggatc ggcggcgtgg
      601 tcggcatcat cgtcgccacc gtcgcattcg tggttctgag gttcctcggc acgagtgcgc
      661 tgatcgaagg cgtgacgcgc gcgcgccgca acggccgcat gaccgtgcgc gaaggattcc
      721 gggaaggttg ggcgaactgg ggcgtgctct ttctcatcac gctggtcttt ctgccgatga
      781 accttgcgac agtcgcgctg ctcggcggcg cgtgctatct cgtctggacg gtattcggct
      841 cgccggccgc gatcttggcg gcctgcgctg cgctcctcct tggcgtcccg tgcctcctga
      901 cgctctacat gtggcagatg ttcgccgagc gcattgccgt gctcgagaat cgccgcgcgc
      961 tcgacgcgat gcgcaaagcg cgtctgttcc tgcacggccg cttgcagctc gggctcaagt
     1021 tgttcgtcgc agaccttctc ggcggtctgt tcgtcacggt cgtcggcctg atcgtgatcg
     1081 ttccgatcgc gttcgtcttc gtatcggctc gcgtgtgggc ggctagcgag tggggcgcgc
     1141 tcgctgcggt cgggctcggt gtgctcacgg tcgcgccatt gctgttcgtg gcgtttgcgt
     1201 tcttcggcat catgacgacc tcggtttgga cgatcggcta cctgacgcag gtggagcaat
     1261 gatcttgacg gcaaccatgg ccgcattgcc ttcggcaccg ttttttctcc ggatctacaa
     1321 cgtcctgtcg gtactcttcg gcggcgacgc cgagccgctc gaggaggacg ccgagctgcg
     1381 gctcgtcgag ctagcgcgcg ccggcgatcc tgtcgcccgt cagcgcctct atcggcgaca
     1441 tgtgcagcgc gtcttccgca cggtgcgcgg catgctgcgc tctgatgccg aggccgagga
     1501 cgtcacgcag gacgcaatgc tgagcgcaat gacttcgctc gatcgttata gcccacgttc
     1561 cggcacccgc ttcgtcgcgt ggctgacgac gatcgccgtc aacacgacgc gtcgccggtt
     1621 tcgccgccgg cgtcccgagt tgaccgcgac gggcgagtta ccggacgtcg tggatgcgga
     1681 cagcgacctc gcaagcgacc tcgacctcgc gcgtcagcga ggcgcgctga tgcaagcgct
     1741 ggccgagctc gagccgcgcg agcgcgcgat cgtcagcctt cgttacggtt cggacctcaa
     1801 tgccaccgaa atcgctgcgc agatgggaat ggaagccgcg aacgtgcgca agattctcga
     1861 acgagtgcga gcccgcctcg gtgagcgcat cgaagcactc gccaaatctc gcggagcagc
     1921 agcatgaacg acgccgaaat catgaatctg tggtgcgcgc tcgagccgag ctcgcgccgc
     1981 cgcgcgcgca tcgagacgcg cgtgttcgag tggctcgagg cgagcgaaac attggtcgcc
     2041 gccgaatggc tgcgcttgct gaaggtcaag ccgctgacga cgctaaccta cgcggcggtg
     2101 gcggcactcg cgctcctggc gctgacgccg gtaggatgga tcgcggcgtg gctgttgccg
     2161 tagctgtcac accggcgcgc gacgcgacga tggcgcgcgg gttcggctgg ctctaagcgc
     2221 agtcgatgtc gagcgagtcg aagacgaagc tgcagccgag cttcgcggcg agcgcctgcg
     2281 cgccgtcgct ccttggatcg atccacaggt aacggttctt gacgccgcgc tcgtcgagca
     2341 aggtgttttc gttgcagagc gtgccggcgc gagcggacga gccgaggcga ggtgtcgcgc
     2401 agtacgcggg gttctcgctg catgcgggca ttggcgtgga atccgagcag cgcgagaagc
     2461 tcgaaagact cacccgctat
//
LOCUS       DASSNN010000511         4772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_322775, whole genome shotgun sequence.
ACCESSION   DASSNN010000511 DASSNN010000000
VERSION     DASSNN010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322775"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFL84_09505"
     CDS             complement(<1..529)
                     /locus_tag="VFL84_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET7608900.1"
                     /translation="MALGRWLLRNVLRLAYAVRVTGLEHYAEAGPRVLIVANHTSFLD
                     AALLAVFLPDRLTFAVNTEIAKRWWMKPLLKIVDAFPLNPTNPYSLKSLIRYIEQDKR
                     AAIFPEGRITVTGSLMKIYPGPGLVADKSKAMVLPIRIDGAQYTPFSRLRGRVRLRLF
                     PRITLTIMPPRRLEIP"
     gene            complement(692..3025)
                     /locus_tag="VFL84_09510"
     CDS             complement(692..3025)
                     /locus_tag="VFL84_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7608901.1"
                     /translation="MMQLLADPIMLLAALVVVLLICALVWAGSRRPYVREVERLRDDL
                     HNLLASGEQAERIPVNGRLSPFVDITASMNRLLDRSADAARVAAQVAEPPTGDRGELF
                     DALADTLPEVALIHTQTILYANRAAGELFGVAPDALIGKPITDLVRPAYRAGMRKHVG
                     PDAESTEPLAPFEVQLISNDEQGIWAELHSRRVMYGGEPALLTVARDITHRKSLETSL
                     GRGKLQARITLESIGEGVITTDRNGTIDYMNEAAEQLIGGARSAGIGKRLGDLISLVD
                     EIDRSTLGDPVAKCLSERRRVNLGRRAFLLSKLAEREFSTELTASPIRAPDGQIAGCV
                     VIFHDVSELRGLAREMSYQASHDALTGLVNRAEFERRLETALDSARGEAVGHVVCYLD
                     LDRFKLVNDTGGHIAGDNLLREIGALLKQRVRDSDTVARVGGDEFAMLLAGCPLDKAQ
                     QIAEDVVQAVAGHQFAWQDRVFDIGVSVGLVEVGKDSGSAESVLSAADSACYIAKQQG
                     RGRTHVYSTRDEVTARERGEIQWLQRLQRALKENGFELYVQPIVAMGGRKSNGPAAEV
                     LLRMRDESGSSILPVHFLASAERYQLMSHIDRWVVQTTLTAIAGGAPHLPAGRTCNIN
                     LSAQTLGDDDFLEFVVDVLDHTGVSPDRICFEVRESAVVNQLDQAQRFIAVLHGIGCR
                     FALDNFGSGIGSFANLKNLSLDFLKIDGTYTRNLDSDTVNREMVAAMVKLARTLDFQV
                     VAEQVEDQTSFEALRDLGVDFVQGYAVEKPRPLHGVH"
     gene            3416..4066
                     /locus_tag="VFL84_09515"
     CDS             3416..4066
                     /locus_tag="VFL84_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HET7608902.1"
                     /translation="MNFDVARTQMLGQQLRAWEVLDDRVLRAFAETPRENFVPREYRD
                     LAFADTEIPLPHGQTMLAPKVEGRILQALQVEPIDEVLVVGTGSGYLAACLGRLAKRV
                     TSVDIFPEFATAAAPKLAACGIRNVRLETADALSLTYRAQFDAIAVTGSVPTLDDHFV
                     SMLRPQGRLFIVVGREPAMEARLITLQPDGTTTSESLFETVLAPLINAERPEPFVL"
     gene            4118..4426
                     /locus_tag="VFL84_09520"
     CDS             4118..4426
                     /locus_tag="VFL84_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7608903.1"
                     /translation="MQFTARWPSYAQGGDVVLLDVREHDELAIAAVEGAVHIPMREVP
                     GRLAELDAGKPVVVMCHSGGRSRRVAEFLKGNGFPDVFNLNGGIDAWSTQIDSRVPRY
                     "
     gene            4568..>4772
                     /locus_tag="VFL84_09525"
     CDS             4568..>4772
                     /locus_tag="VFL84_09525"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7608904.1"
                     /translation="MNDPAIREAEASYLATAEVKPQARSQILPTLTLGVNRSHSFNDT
                     EVRSGAIDPITGFPLGSRQQSEQD"
BASE COUNT          818 a         1605 c         1541 g          808 t
ORIGIN      
        1 ccggaatctc gagccgccgc ggcggcatga tcgtcagcgt gatacgcggg aagagccgca
       61 gccgcacgcg tccgcgcagg cgcgaaaaag gcgtgtactg cgcgccgtcg atgcggatcg
      121 gcagcaccat tgccttcgac ttgtccgcga cgagacccgg gccgggatag atcttcatca
      181 gagagcccgt caccgtgatg cggccttccg ggaagatcgc ggcgcgcttg tcctgctcga
      241 tgtagcggat cagcgatttc agcgagtacg gattcgtcgg gttcagcggg aacgcgtcga
      301 cgatcttgag cagcggcttc atccaccagc gcttggcgat ttcggtgttg accgcgaacg
      361 tgaggcggtc gggcagaaac acggccagca gcgcggcatc gaggaacgac gtgtgattcg
      421 cgacgatcag aacgcgcggc ccggcttccg cgtaatgctc gaggcccgtg acgcgtacgg
      481 catacgcgag gcgcaggaca ttgcgcagca accatcgacc gagagccacc atcgggagcc
      541 gtctcgcacc acttcgttgc cgctacgatg cccgcagcgc cggccaattg ccatccgaaa
      601 aggcagcatc cgaaaagggg acacgaaaac ggggacagat ctattttcct ccgaaaatag
      661 atctgtcccc gttttcgtgt ccccgttttc gctagtggac gccgtgcagc ggccggggtt
      721 tctcgaccgc gtagccttgg acgaagtcga cgccgaggtc gcgcagcgct tcgaacgacg
      781 tctggtcctc gacctgctcg gcgacgacct ggaagtcgag cgtccgcgcg agcttgacca
      841 tcgcggcgac catctctcga ttcacggtgt cgctgtcgag gttgcgcgta taggtcccgt
      901 cgatcttcag gaagtcgagc gacaggttct tcaagttcgc gaacgagccg atgccgctgc
      961 cgaagttgtc gagcgcgaac cggcagccga taccgtgcag caccgcgatg aagcgctgcg
     1021 cctgatcgag ctggttcacg acggccgact cgcgaacctc gaagcagatg cggtcagggc
     1081 tgacgccggt gtggtcgagc acgtcgacga cgaactcgag gaagtcgtcg tcgccgagcg
     1141 tctgcgccga caggttgatg ttgcacgtgc gccccgccgg cagatgcggc gcgccgcccg
     1201 cgatggccgt caacgtcgtc tgcactaccc agcggtcgat gtgcgacatg agctgataac
     1261 gctcggccga tgcgaggaag tggacgggca ggatcgagct gccgctctcg tcgcgcatgc
     1321 gaagcaggac ctcggcggcc gggccgttgc tcttccgtcc gcccatcgcg acgatcggct
     1381 gcacgtagag ctcgaagccg ttttccttga gcgcgcgctg cagccgctgc agccactgga
     1441 tctcgccgcg ttcgcgcgcc gtgacctcgt cgcgcgtcga gtagacatgc gtgcggcccc
     1501 gcccctgctg cttcgcgatg tagcacgcgg agtccgcggc actgaggacg ctctccgcag
     1561 agccgctgtc cttgcccact tcgaccagcc cgacgctgac gccgatgtcg aagacgcggt
     1621 cttgccatgc gaactgatgc ccggcgacgg cctggacgac gtcctcggcg atctgctgcg
     1681 ccttgtcgag cgggcagccc gcaagcagca tcgcgaactc gtcgccgccg acgcgcgcga
     1741 cggtgtccga atcgcgaacg cgctgcttca acagcgcgcc gatctcgcgc agcaagttgt
     1801 cgccggcaat gtgccctccg gtgtcgttga cgagcttgaa ccggtcgagg tcgagatagc
     1861 agacgacgtg cccgacggct tccccgcgcg cggagtcgag cgcggtctcg agccgccgct
     1921 cgaactcggc gcggttgacg aggcccgtca gcgcgtcgtg gctcgcttga tacgacatct
     1981 cgcgcgcgag ccctcgcagc tcgctgacgt cgtggaagat cacgacgcaa ccggctatct
     2041 ggccgtccgg cgcgcggatc ggcgacgccg tgagctccgt cgagaactcg cgctcggcca
     2101 gcttgctcag caagaacgcg cgccggccga gatttacgcg ccgccgctcg ctcaagcact
     2161 tcgccaccgg atcgccgagc gtgctgcggt cgatctcgtc gacgagcgag atcagatcgc
     2221 ccaggcgctt gccgattccg gcgctgcgcg cgccgccgat cagctgctcg gccgcctcgt
     2281 tcatgtaatc gatcgtgccg ttgcggtccg tcgtgatgac gccttcgccg atcgattcga
     2341 gcgtgatgcg ggcttgcagc ttgccgcggc cgagcgacgt ctcgaggctc ttgcggtgcg
     2401 tgatgtcgcg cgcgacggtc aagagcgcgg gctctccccc gtacatgacg cggcggctgt
     2461 ggagctcggc ccagatgcct tgctcgtcgt tgctgatgag ctgcacctcg aacggggcca
     2521 acggctcggt gctctccgca tcggggccga cgtgcttgcg cattccagcg cggtacgccg
     2581 gacgcacgag gtccgtgatc ggcttgccga tcagcgcatc gggcgcgacg ccgaagagct
     2641 cgcccgccgc tctattcgca tacaggatcg tctgcgtgtg gatcagcgcg acctcgggca
     2701 gcgtgtcggc caaggcgtcg aagagctcgc cgcgatcgcc ggtgggcggc tccgcgactt
     2761 gcgccgcgac gcgcgcggca tcggcgctgc gatcgagcag gcggttcatc gacgccgtga
     2821 tgtcgacgaa cgggctcaag cggccgttga cggggatgcg ctcggcctgc tcgcccgacg
     2881 ccaggaggtt gtgcaaatcg tcgcgcagcc gctcgacttc gcgaacgtaa ggcctgcgcg
     2941 agccggccca tacgagcgcg cagatgagca gcacgacgac gagcgcggcg agcagcatga
     3001 tcggatcggc gagcagctgc atcatcctcc cccgcctgcg accccgtcgt tcggcacgcg
     3061 gccgtccgat cggcgccggc gcgctgcaag tgcaaacggg aatcgatcga cggacagcga
     3121 gatccgtgct gacacgtgat tgcgaagccc catgtgttgc cgcgcttcgc tgcgcggatg
     3181 cctgttcttg ttgttagcgc gcatcccgcg cgtcgggcgg ccttgcaccg agcctaaaac
     3241 tcggtcgccg gatggccgct tctcctcgcg ttcgacgata caggaagcgc ccgaaaagtc
     3301 gctcgtaatt gaccataatt cttcacatac cgccttgtat cgcatgaata aacttcttaa
     3361 cctaccgcgt tcttcacgag cgccctcttt tctccaagaa cacgccaaga acacgatgaa
     3421 cttcgacgtt gcacgaaccc aaatgctcgg ccagcagctg cgggcctggg aagtgctcga
     3481 cgatcgcgtt ctgcgcgcgt tcgcagaaac gccgcgcgag aatttcgtcc cgcgcgaata
     3541 ccgcgatctc gccttcgccg acaccgagat cccgctcccg cacggccaga cgatgctggc
     3601 accgaaagtc gaagggcgca tcctccaggc cctgcaggtc gagcccatcg acgaggtgct
     3661 cgtcgtcgga accggcagcg gctatctcgc ggcgtgcctc ggtcggctcg cgaagcgggt
     3721 cacgagcgtc gacatctttc ccgaattcgc gacagccgca gcgccgaagc tcgcggcctg
     3781 cggcattcgc aacgtccggc tcgagacggc cgacgcgctg agcctgacct accgggcgca
     3841 gttcgacgcg atcgccgtca ccggctccgt ccccacgctc gacgaccact tcgtctcgat
     3901 gctgcgcccg caagggcgcc tgttcatcgt cgtgggccgc gagcccgcca tggaagcccg
     3961 gctcatcacg ctgcagccgg acggcacgac gacgagcgaa agcctcttcg aaacggtgct
     4021 cgcgccgctg atcaacgccg agcggccgga gccgttcgtc ctctaagcaa gcgacgttag
     4081 agtaattctc atgccgggcg ttccggagct aagccccctg caatttaccg cgcgctggcc
     4141 gagctacgcg caaggcggcg acgtcgtgct gctcgacgtg cgcgagcacg acgagctcgc
     4201 gatcgcggca gtcgaaggcg ccgtgcacat cccgatgcgc gaggttcccg ggcgacttgc
     4261 cgagctcgac gcgggcaaac cggtcgtcgt gatgtgccat tcgggtggcc gcagccggcg
     4321 cgttgctgaa tttttgaaag gaaacggctt tccggacgta ttcaatctca acggtggtat
     4381 cgacgcgtgg tcgacgcaga tagactcccg ggtaccgcgc tattagtcac cccccataac
     4441 gacgtctaga cagggataac aagcgatgtc aattacgaag cacttactag cgcttaccct
     4501 cggcgcgtgc ctgaccacgc aggtttcggc gcaggatagc ctcctcgatg tctatcaacg
     4561 cgcgctgatg aacgacccgg ccattcgcga ggccgaggcc agctatctcg cgacggccga
     4621 agtgaagccg caggcacgca gccagatctt gccgacgctc acgctcggcg tgaaccggtc
     4681 gcactcgttc aacgatacgg aggtcaggag cggcgccatc gaccccatca cgggcttccc
     4741 gctcggctct cgacagcagt cggagcagga cg
//
LOCUS       DASSNN010000512         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_952609, whole genome shotgun sequence.
ACCESSION   DASSNN010000512 DASSNN010000000
VERSION     DASSNN010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952609"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFL84_09530"
     CDS             <1..405
                     /locus_tag="VFL84_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7608905.1"
                     /translation="LNVLEHIEDDVGALRTLRAKLVPRGRLFVYVPAFPVLYTSMDAR
                     VRHIRRYTRTTLRDSLAAAGFEILELRYADSLGFAATLLFKALDNGRGDVNRGLLRLY
                     DRLAFPVSRALDALTHRWFGKNLLALAAKPAA"
     gene            complement(412..831)
                     /locus_tag="VFL84_09535"
     CDS             complement(412..831)
                     /locus_tag="VFL84_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7608906.1"
                     /translation="MSARRAVDVVVGLIGDSSGRWLVNCRPPGTPLAGSWEFPGGKTR
                     PGEAAFAALARELDEELGIEVFEAEPVLVLEHDYPDKRVRLDVWHVVKFRGRATPREG
                     QPLRWVTAAECLDLPLLPADGPIVARLASLGASSEVP"
     gene            complement(860..>2506)
                     /locus_tag="VFL84_09540"
     CDS             complement(860..>2506)
                     /locus_tag="VFL84_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HET7608907.1"
                     /translation="KEGVRIQNESITYATITLQNYFRLYDKLAGMTGTASTEGEEFYE
                     IYKLEVVTIPTHRPMIREDYNDLVYRTERAKFEAVAEEIAELQEEGRPVLVGTVSIEK
                     SEYLSELLTRKGIQHQVLNAKQHEREAGIVAQAGKYAGVTIATNMAGRGTDIVLGGKP
                     DGRPKEEWEDEHNRVVEAGGLEIIGTERHESRRIDNQLRGRSGRQGDPGSSRFYLSME
                     DNLMRLFGDPERIKRMLSRAGMKEGEAIESKLLSRQIERAQRKVEAHNFDIRKQLLEY
                     DDVANDQRRVVYQQRQELMEASDIAEAIGAIRAEVVNNAISEYVPPESVEEDWNKEGL
                     TKALEREFGVRMDIASLLERDHTIDEKKLRDLVVGAVSEAYEKKLAEIGPQVMHEVEK
                     QIMLRQLDSHWKEHIAALDYLRQGIHLRGYAQRNPKQEYKREAFEMFSTMLERVKHDT
                     ISILSKIQIRRPEDVQAVEPAAPDPRTLRFQHAAAPSLTEAPPAPEPRPSAAQPDLRM
                     RPPPPEPVAPYVREAPKVGRNDPCPCGSGKKFKQCHGRLT"
BASE COUNT          333 a          870 c          824 g          479 t
ORIGIN      
        1 ctgaacgtgc tcgagcacat cgaggacgac gtcggggcgt tgcgcacgct gcgcgcgaag
       61 ctggtgccgc gcggccgctt gttcgtctac gtgcccgcgt tccccgtgct ctacacgagc
      121 atggatgcgc gagtgcgcca cattcggcgc tacacgcgca cgacgctgcg cgacagcctc
      181 gcggcggccg ggttcgagat cctcgagctg cgctacgccg actcgctcgg cttcgccgcc
      241 acgctgctgt tcaaggcgct cgacaacggc cgcggcgacg tcaatcgcgg cttgctgcgc
      301 ctctacgacc ggttggcgtt ccccgtcagc cgcgcgctcg acgcgctgac gcaccgctgg
      361 ttcggcaaga atttgctcgc gctcgcggcc aagcccgcgg cctgacgctc gtcagggaac
      421 ttcgctcgac gcgccgagcg acgcgagccg cgcgacgatg ggcccatcgg ccggcagcag
      481 cggcaaatcg aggcactccg cggcggtgac ccagcgaagc ggttgtccct cgcgcggcgt
      541 cgctcggcct ctgaacttca cgacgtgcca gacgtcgagc ctgacgcgct tgtcggggta
      601 atcgtgctcg agcacgagca cgggctcggc ctcgaacacc tcgatcccga gctcctcgtc
      661 gagctcgcgc gcgagcgcgg cgaacgccgc ttcgccgggg cgcgtcttgc cgcccggaaa
      721 ctcccacgag cccgcgagcg gggtgccggg cgggcggcaa ttgacgagcc aacgcccgct
      781 cgagtcgccg attaggccga cgacgacgtc gacggcgcgc cgcgcgctca tgacccttgg
      841 agctcctcga acgagcgcgt caggtcagac ggccgtggca ctgtttgaat ttcttgcccg
      901 agccgcacgg gcacgggtcg ttgcggccga ccttcggcgc ctcgcgcaca tacggcgcga
      961 ccggctccgg cggcggcggg cgcatgcgca ggtccggttg cgcggcgctc ggccgcggct
     1021 cgggcgcggg cggcgcctcg gtcagcgagg gggcggctgc atgctggaac cgcagcgtcc
     1081 gcgggtcggg cgcggccggc tcgacggcct ggacgtcctc gggccggcga atctggatct
     1141 tcgacaggat gctgatcgtg tcgtgcttca cgcgctcgag catcgtgctg aacatctcga
     1201 acgcttcgcg cttgtactct tgcttcggat tgcgctgcgc gtaaccgcgc aagtgaattc
     1261 cttggcgcaa gtaatcgagc gccgcgatgt gctctttcca gtgcgagtcg agctggcgca
     1321 gcatgatctg cttctcgacc tcgtgcatga cctgcggtcc gatctcggcc agcttcttct
     1381 cgtaggcctc ggagacggcg ccgacgacga gatcgcgcag cttcttctcg tcgatcgtgt
     1441 ggtcgcgctc gagcaatgac gcgatgtcca tgcgcacgcc gaactcgcgc tcgagcgctt
     1501 tggtcaggcc ttccttgttc caatcctcct cgacgctctc gggcggcacg tactcgctga
     1561 tcgcgttatt cacgacctcg gcgcgaattg cgccgatcgc ttccgcgatg tcgctcgcct
     1621 ccatgagctc ctgccgctgc tggtagacga cgcgccgctg atcgttcgcg acgtcgtcgt
     1681 actcgaggag ctgcttgcgg atgtcgaagt tgtgcgcctc gaccttgcgc tgcgcgcgct
     1741 cgatttgccg cgacaacagc ttgctctcga tcgcctcgcc ttccttcatg cccgcgcgcg
     1801 acagcatgcg cttgatgcgc tcggggtcgc cgaagaggcg catcaggttg tcttccatcg
     1861 acagatagaa gcggctggaa cccgggtcgc cctgacggcc cgatcgtccg cgcagctggt
     1921 tgtcgatgcg ccgcgattcg tgccgctcgg tgccgatgat ctcgagcccg ccggcctcga
     1981 cgacgcggtt gtgctcgtcc tcccactctt ccttcgggcg gccgtcgggc ttgccgccaa
     2041 ggacgatgtc ggtgccgcgg cctgccatgt tcgtcgcgat ggtgacgccc gcatacttgc
     2101 cggcctgcgc gacgatgcct gcctcgcgct cgtgctgctt tgcattcagc acctggtgct
     2161 gaatgccctt gcgtgtcagc agctccgaca ggtactccga cttctcgatc gacaccgtgc
     2221 cgacgagcac cggccgccct tcctcctgca gttcggcgat ctcctccgcg acggcctcga
     2281 acttcgcgcg ctccgtgcgg tagacgaggt cgttgtagtc ctcgcggatc atcgggcggt
     2341 gcgtggggat ggtgacgact tcgagcttgt agatctcgta gaactcttcg ccctcggtcg
     2401 atgccgtacc ggtcatgccg gcgagcttgt cgtagaggcg gaagtagttc tgcagcgtga
     2461 tcgtcgcgta cgtgatcgac tcgttctgga tccgcacgcc ttcctt
//
LOCUS       DASSNN010000513         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1602648, whole genome shotgun sequence.
ACCESSION   DASSNN010000513 DASSNN010000000
VERSION     DASSNN010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602648"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VFL84_09545"
     CDS             complement(<1..702)
                     /locus_tag="VFL84_09545"
                     /inference="COORDINATES: protein motif:HMM:NF017447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7608908.1"
                     /translation="MTRAVNVADIIDTRPVGAFQLRAFLLCALVLFVDGFDVQGITYV
                     VPTITREWGLVRGQLWPALTAGLVGVMLGAMLLAPLADRVGRRRVIVYSCVAFGALTL
                     LTLAADSLLSLVVLRFLTGLGLGSALPNALGLASEYAPHKHRALVVMFVGSGISLGAI
                     AAGIAATQLVGPFGWRAMFVVGGLLPLALAVLLASGLPESIRFAALLANRRDEAKRLL
                     RKLAPALDNDIDVVSH"
     gene            855..1391
                     /locus_tag="VFL84_09550"
     CDS             855..1391
                     /locus_tag="VFL84_09550"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HET7608909.1"
                     /translation="MQNRNPYAAPRTNVTQRDSTEDYGEIKLFSAEGRIGRVRYIGYT
                     FGLTILIALFIGMAAALLGAASPSIAFGVGAVGYIAIIVVQVLLTIQRSHDMNVTGWL
                     CLISLIPLAALVFWLVPGTRGENNYGKPPPPNTAGAVVLACILPVIMIVGIVAAVAIP
                     AYQQYQQRVAESPVQQLE"
     gene            1423..2310
                     /locus_tag="VFL84_09555"
     CDS             1423..2310
                     /locus_tag="VFL84_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HET7608910.1"
                     /translation="MQNRNPYAAPRTNVTARDSAVAEYGEVKLFSASGRIGRVRYLAY
                     SIGLSIIVLLVAGMMAGLAGVIGGEGAAVLVMALGYFAMLGIQFLPAIQRSHDMNVSG
                     WLSLIQLVPFGPLVFLFAPGTRGENNYGNPPPPNGVGVILVACLLPFFFVGGVLAAIA
                     IPAYQDYTIRAQVSEGLNIAAAIKSEVAQAYGRTGAAPADRVEAGLSADPADSAGMYV
                     DSVDVMQGTIMITYGGNANGAIAGSVLAIRPYVMPDRSVVWRCGQGPVPVGAVAMDES
                     ATAFTVDMLPKYLPSACRP"
     gene            2388..>2679
                     /locus_tag="VFL84_09560"
     CDS             2388..>2679
                     /locus_tag="VFL84_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7608911.1"
                     /translation="MPRRHSVLALAALAAVSLSSHAQQPPTAAAAPTESVLDVHGGQI
                     RLVTVASGLVHPWSIALLPGDASMLVAERNGKVRLIQNDALAAEPVWSADGVA"
BASE COUNT          447 a          924 c          874 g          434 t
ORIGIN      
        1 gtgcgacacg acatcgatgt cgttatcgag cgccggcgcg agcttgcgca gcaggcgctt
       61 cgcctcgtcg cggcggttgg cgagcagcgc cgcgaaacgg atcgactccg gcaacccgct
      121 tgcgagcagc accgcgagcg ccaacggcag gaggccgccc acgacgaaca tggcgcgcca
      181 accgaacggc cctacgagct gcgttgcggc gattccggcg gcgatcgcgc cgagcgagat
      241 gccgctgccg acgaacatga cgacgagcgc gcgatgcttg tgcggcgcgt actccgaggc
      301 gaggccgagc gcgttcggca aagcgctgcc gagcccgagg ccggtcagaa aacgcagcac
      361 cacgagcgag agcaacgagt cggccgctag agtcagcagc gtcagcgcgc cgaacgcgac
      421 gcacgaatac acgatgacgc ggcgcctacc cacgcgatcg gcgagcggcg ccagcaacat
      481 cgcgccgagc atgacgccga cgagcccggc cgtgagcgcg ggccagagct ggccgcgaac
      541 gagcccccat tcgcgcgtga tcgtcggcac gacgtacgtg atgccttgca cgtcgaagcc
      601 gtcgacgaac agcaccaggg cacagagcaa gaaggcgcga agctgaaacg cgccgacggg
      661 gcgcgtatcg atgatgtccg caacgttgac ggctcgggtc acgcttcagc tcttgctagg
      721 tcggtgtggt gacgtaagtc cgccgtaatc ataaacgact gtgaactgac ttgctttttc
      781 gcggccgccg actccctaag atgcatccgc atccgggaag gctctgaagc ccacagcccc
      841 acgaggcatt cgtcatgcaa aaccgcaacc cctatgccgc tccgcgcacg aacgtgacgc
      901 agcgcgacag cacggaagat tacggcgaga tcaagctgtt ctccgccgaa ggccgcatcg
      961 gccgcgtgcg ctacatcggc tatacgttcg gtctcacgat tctgatcgcg ctcttcatcg
     1021 gaatggccgc tgcgttgctc ggtgccgcaa gcccctccat cgcgttcggc gtcggcgccg
     1081 tcggctacat cgcgatcatc gtcgtgcagg tcctgctgac gattcagcgc tcgcacgaca
     1141 tgaacgtgac cggctggctc tgcttgatct cgctgattcc gctggccgcg ctcgtgttct
     1201 ggctcgtgcc cggcacgcgc ggcgagaaca actatggcaa gccgccgccg ccgaacacgg
     1261 ccggcgccgt cgtgctcgct tgcatcctgc cggtcatcat gatcgtcggc atcgtggccg
     1321 cggtcgcgat ccccgcgtat cagcagtatc agcagcgcgt cgccgagtcg cctgttcagc
     1381 agctcgagta gcgctcgcga ccgccgaact cgagggggcc tcgtgcagaa ccgcaatccc
     1441 tatgccgcgc cgcgaacgaa tgtgacggcg cgcgacagcg ccgtcgccga atatggcgag
     1501 gtcaagctct tctcggcgag cggccgaatc ggccgcgtcc gctacctcgc gtactcgatc
     1561 gggctttcca tcatcgtctt gctcgtcgcc ggcatgatgg ccggactggc cggcgtgatc
     1621 ggcggcgagg gcgccgccgt cctcgtcatg gcgctcggct acttcgcgat gttgggcatc
     1681 cagttcctac cggcgatcca gcgctcgcac gacatgaacg tgtcgggctg gctctcgttg
     1741 atccagctgg ttccgttcgg cccgctcgtg ttcttgttcg cgccgggcac tcgcggcgag
     1801 aacaactacg gcaatccgcc gccgccgaac ggcgtcggcg tgatcctcgt cgcgtgtctc
     1861 ctgccgttct tcttcgtcgg cggcgtgctg gccgcgatcg cgattccggc ctatcaggac
     1921 tacacgatac gggcacaggt ctccgaaggc ctcaacatcg cggcggcaat caaatccgag
     1981 gtcgcacagg cttacggccg aacgggagcc gcgccggcgg atcgtgtcga ggccggtctg
     2041 tccgcagatc ccgcggattc ggccgggatg tacgtcgata gcgtcgacgt catgcagggc
     2101 acgatcatga tcacctatgg cggcaacgcg aacggggcta tcgccggcag tgtcctcgcc
     2161 attcggccgt acgtcatgcc ggaccgcagc gtggtctggc gctgcggcca gggcccggtg
     2221 ccggtcggcg ccgtggcaat ggacgagagc gcgacggcct tcaccgtcga catgctgccc
     2281 aagtatctgc cctcggcctg tcgcccctga ttcaacgaac gcattgaccc ccgccgatcg
     2341 gggtctatga tccgagcgtt gcaagcctcg aggggagggc tttcgcaatg ccgcgccgac
     2401 attccgttct cgcgctcgcc gcgctcgccg ccgtgtcgct gagctcgcac gcccaacagc
     2461 caccgaccgc ggcggccgcg ccgaccgaaa gcgttctcga cgtgcacggc gggcagatcc
     2521 gcctcgtgac cgtagcgtcg ggcctcgtgc atccgtggag catcgcgctg ctgccgggcg
     2581 acgcctcgat gctcgtcgcc gagcgcaacg gcaaagtgcg gctcatccaa aacgatgcgc
     2641 tcgccgccga gccggtgtgg tcggccgacg gagtggcgg
//
LOCUS       DASSNN010000514         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_593335, whole genome shotgun sequence.
ACCESSION   DASSNN010000514 DASSNN010000000
VERSION     DASSNN010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593335"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VFL84_09565"
     CDS             complement(<1..503)
                     /locus_tag="VFL84_09565"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608912.1"
                     /translation="MDDAARTLALDRVNRQFLLEHPAQAADVIDGFGRAAAVQVISGH
                     QADTLATVWRHLSPARIDEIVPELPEKLLKQALHALDPGVLAISLPRISEESRNRCLA
                     LLPRRAEAEVRRLMDYPAGSAGRVMDKRIPSFRGEQPVALTLEQLRQSKPKDLRELFI
                     VDAERRL"
     gene            complement(509..1336)
                     /locus_tag="VFL84_09570"
     CDS             complement(509..1336)
                     /locus_tag="VFL84_09570"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7608913.1"
                     /translation="MSGREELAAAYLRLHPEAAARLLESMPADQANAVLGAVDAATAA
                     PVVGHMLPTCAAACIEQQPPAEAAQLFERLGSRESVAVLRHLPNELRENVLSSLGPQW
                     LMAFKLLLSYPTNTVGAWIEPRVLTLPDDCTAGEARDRVARSSSQAQARIYVLDRARR
                     LRGAVRGVGLLQVSSRKKIVSLLEPAEALWAREPLATAQEHGVWERHTEAPVVNRDEE
                     FIGVISYADLRKAFREIHHVAGPNGDRDMAEVSELFAIGAGSLWRSIGDLIGNHRQR"
     gene            complement(1333..>2047)
                     /locus_tag="VFL84_09575"
     CDS             complement(1333..>2047)
                     /locus_tag="VFL84_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608914.1"
                     /translation="WLIAHVIRGLVRRALESMDWLFARFAPRSAGRSEALARATSRAI
                     STVVFWIVLLVFAASALRILGGSLFQRWTENLLAYLPSAIGGIIIIVVGFTAGTLVRH
                     VLEQASVGLGVGQSSLLGRLAQAVIVISCIVIGIDQLGVDVAFLVQLATVTAAAVFGG
                     IALVFALGTRQHLANLIGAHYARKHYAPGDFVKIGAFQGRIVEIVDGCVFLETEAGDV
                     SVPAQNFSAEAFVKIRKTS"
BASE COUNT          324 a          700 c          731 g          292 t
ORIGIN      
        1 atgaggcggc gttcggcgtc gacgatgaag agctcgcgca ggtctttcgg cttgctctgg
       61 cgcagctgct cgagcgtcaa ggcgacgggc tgctcgccgc ggaagctcgg gatccgcttg
      121 tccatgacgc ggcccgccga accggccggg tagtccatga gccggcgcac ttcggcctcg
      181 gcgcgccgcg gcaagagcgc gaggcagcgg ttgcgggatt cctcggagat gcgcggcaac
      241 gagatcgcga gcacgccggg gtcgagcgcg tgcagtgcct gcttcagcag cttctcgggc
      301 agctccggga cgatctcgtc gatccgcgcc ggcgagagat gccgccagac ggtcgcgagc
      361 gtgtcggcct gatgtccgga gataacctgc acggcggccg cgcgcccgaa gccgtcgatg
      421 acgtccgcgg cttgcgcggg gtgctcgagc aggaactggc gattgacccg gtcgagcgcg
      481 agcgtgcgag cggcgtcatc catgcccatt accgttgccg gtggttgccg atcaggtcgc
      541 cgatgctacg ccacaagctg ccggcgccga tagcgaagag ctccgacacc tcggccatgt
      601 ctcgatcgcc gttcgggccc gcgacgtgat gaatctcgcg gaacgccttg cgcagatccg
      661 cgtacgaaat cacgccgatg aactcctcgt cgcgattgac gaccggcgct tcggtgtgcc
      721 gttcccaaac gccgtgctcc tgcgccgtcg cgagcggctc gcgtgcccac agggcctcgg
      781 ccggctcgag cagcgagacg atcttcttgc ggctcgagac ttgcagcagc ccgacgccgc
      841 gcacggcgcc gcgtaagcgg cgcgcccggt cgagcacgta gatgcgcgcc tgcgcttggc
      901 tcgagctgcg cgcgacacgg tcgcgcgctt cgccggccgt gcagtcgtcg ggcagcgtca
      961 gcacgcgcgg ctcgatccag gcgccgacgg tgttcgtcgg atagctcaag agcagcttga
     1021 aagccatgag ccactgcgga cccagcgagg agagcacgtt ctcgcggagc tcgttcggca
     1081 agtgccgcag cacggcgacg gactcgcggc tgcccagccg ctcgaatagc tgcgcggctt
     1141 ccgccggcgg ttgctgctcg atgcaggccg ccgcgcaggt cggcagcatg tggccgacga
     1201 cgggcgccgc cgtcgcggca tcgacggcgc cgagcacggc gttcgcttga tcggccggca
     1261 tcgactcgag cagacgcgcg gcggcctcgg ggtgcaggcg caagtaagcg gcggcgagct
     1321 cctcgcggcc gctcacgacg tcttcctgat cttcacgaac gcctcggcgc tgaagttttg
     1381 cgccggcacc gagacgtcgc ccgcttcggt ctcgaggaac acgcagccgt cgacgatctc
     1441 gacgatgcgg ccttggaacg cgccgatctt cacgaagtcg ccgggcgcgt agtgcttgcg
     1501 cgcgtaatgc gcgccgatca gattggcgag gtgctggcgc gtgccgagcg cgaacacgag
     1561 cgcgatgccg ccgaacaccg cggccgccgt gacggttgcg agctgcacca ggaaggcgac
     1621 gtcgacgccg agctgatcga tgccgatgac gatgcagctg atgacgatga cggcctgcgc
     1681 cagcctgccg agcaggctgc tctggccgac gccgagcccg accgacgcct gctcgagcac
     1741 gtgccgcacg agcgtgccgg cagtgaagcc gacgacgatg atgatgatgc cgccgatcgc
     1801 ggacggcagg tacgcgagaa ggttttcggt ccagcgctgg aacaggctgc cgcccaagat
     1861 gcgcagcgcg ctggccgcga acacgagcag cacgatccag aacacgaccg tcgagatcgc
     1921 gcggctcgtc gcacgggcca gcgcttcgga ccggcccgcc gagcgcggcg cgaagcgcgc
     1981 gaacagccag tccatgctct cgagggcgcg ccgcacgagc ccgcggatca cgtgggcgat
     2041 cagccag
//
LOCUS       DASSNN010000515         3701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1220327, whole genome shotgun sequence.
ACCESSION   DASSNN010000515 DASSNN010000000
VERSION     DASSNN010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220327"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /gene="tatC"
                     /locus_tag="VFL84_09580"
     CDS             complement(<1..671)
                     /gene="tatC"
                     /locus_tag="VFL84_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET7608915.1"
                     /translation="MSSLPLDPESARLGKRLLAGALELRSRAFKMILCLFGMFLVLLP
                     FADQIFQIVADPIMRAMPEGSSLISKQVASPFLTPLRATFWVALFGSMPLLLYHIWKL
                     VDAWMPKSKRRVALPFIAASATLFYVGVAFAFFVILPIVFQFFASRELADVKIMTDIN
                     AFLSFTLGMVFAFGLAFQMPIVIVIIVWTGLVSRRALKNARPYVLLAVFVIGAILTPP
                     DLFSQT"
     gene            complement(784..1176)
                     /locus_tag="VFL84_09585"
     CDS             complement(784..1176)
                     /locus_tag="VFL84_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET7608916.1"
                     /translation="MSVVIDASVLVAAIVDAGPNGEWAEHLLAQGGLHSPELVLVEAT
                     NIFRRLERAKKITSLEAGAAHRDLVNLDIEVYAFAPVAERVWQLRHALTSYDAWYVAV
                     AEAVELPLATLDTRLTRADGPGCAFLIP"
     gene            complement(1173..1409)
                     /locus_tag="VFL84_09590"
     CDS             complement(1173..1409)
                     /locus_tag="VFL84_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608917.1"
                     /translation="MTVQITIRDVPAHVRDELAARAARQGKSMQEYLRSELERMAARP
                     SIDAWLDGVRRRKEAAKSRVSARDILRSRDADRR"
     gene            1515..2357
                     /locus_tag="VFL84_09595"
     CDS             1515..2357
                     /locus_tag="VFL84_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7608918.1"
                     /translation="MNKKIWLGLTCSCLAFSAQAQFGTEPVELELIKVKDDLYVIHGA
                     YVPGNVTALITNEGVILVDDDFAVDHDNLMKKLREVTREPVKYVINTHFHNDHSGGNA
                     KLKAEGTLAVASIAARTRMLSASPQDAADITVEPRGAVHLGGKVAEIYYFGRGHTDGD
                     VVVLFPHDRTLAAGDLFTHGEGLPQLVDHAGGGSAKEWTGTLDKVLALDFDTVVPGHG
                     TVTNKQAMRDARASSQRMTEMLTQMVKQNRSKADIEAMLRNEFGFQDFHIMMSLDGLI
                     NEMK"
     gene            2397..3146
                     /locus_tag="VFL84_09600"
     CDS             2397..3146
                     /locus_tag="VFL84_09600"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET7608919.1"
                     /translation="MKRPSLLVVAVLALVVAGLASGQFRGRRSAYRYGTFDDNTPPRT
                     EFVNMRWHFGTNGAIGHMGWSHNYPYSELNLNEFIGRTTRVDVEDNSYRGIELSSPEI
                     FQYPFAYISEPGEMEMTEAEVANLREYIERGGFILIDDFDTWHMDNLREEMHRVFPDR
                     NFERLSVDNPIFNLVHEIESLDSMAPYVAGDDPVYYGLDNSMGQIAIVALHNNDFANF
                     WEWYGNPGYPLAPAADAFRMGTNFIVHAMTH"
     gene            3169..>3701
                     /locus_tag="VFL84_09605"
     CDS             3169..>3701
                     /locus_tag="VFL84_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7608920.1"
                     /translation="MTKHTTTRRDFLARSSAAAAAAASLARSAALAAVEEHGIPQRPI
                     PSTGEMLPVIGLGSSKVVEQIAQNGTEPLLGVLRALVAQGGKLVDTWPRNAANDAGFG
                     RVASMPEFRDKLFVTTKVDQVGKEAGVKQFRDSLRNYQRSVIDLVQIFSLTDLDTHWP
                     SLKEWKAEGAARYIGVTV"
BASE COUNT          728 a         1185 c         1209 g          579 t
ORIGIN      
        1 gtctgcgaaa acaagtcggg cggcgtcaag atggcgccga tcacgaacac ggcgagcagc
       61 acgtaaggcc gtgcgttctt caatgcgcgc cgcgagacga gccccgtcca gacgatgatg
      121 acgatgacga tcggcatctg gaacgcgagg ccgaacgcga acaccattcc gagcgtgaag
      181 ctcaagaagg cgttgatgtc ggtcatgatc ttgacgtcgg cgagctccct cgaagcgaag
      241 aactggaaca cgatcggcaa gatcacgaag aacgcgaacg cgacgccgac gtagaacagc
      301 gtggcgctcg cggcgatgaa cggcagcgcg acgcgccgct tcgacttcgg catccaggcg
      361 tcgacgagct tccagatgtg atagagcagc agcggcatcg agccgaacag ggcgacccag
      421 aacgtcgcgc gtagcggcgt caggaacgga gatgcgactt gcttgctgat gaggctcgag
      481 ccttcgggca tcgcgcgcat gatcgggtcc gcgacgatct gaaagatctg gtcggcgaac
      541 ggcagcagca cgaggaacat gccgaacagg cagagaatca tcttgaacgc gcgcgagcgc
      601 agctcgagcg cgccggcgag gagccgcttg ccgagccgag cgctttcggg atcgagggga
      661 aggctggaca cgcggccatt ctaaacgatc gcgcgtgagc ggcgtccgaa gggatccgtc
      721 cgatgttgcg tgacgcggag gtcgtccgac cgcgagctta tggcgagacg ggcctacgga
      781 acgttagggt ataaggaaag cgcagcccgg gccgtcagcg cgcgtgaggc gcgtgtcgag
      841 cgtggccagc ggtagctcga ctgcttcggc cactgcgacg taccacgcat cgtagctcgt
      901 caatgcatgg cgcagttgcc acactcgttc ggcgacagga gcgaacgcgt agacctcgat
      961 gtcgagattt accaggtcgc ggtgcgctgc ccccgcttcg agcgacgtga tcttcttggc
     1021 cctttcgagg cggcggaaga tattggtcgc ttcgacaagg acgagctcgg gtgagtgcag
     1081 tccgccctgt gcgagcagat gttctgccca ttcgccgttc gggcctgcgt caacaatcgc
     1141 cgcgacgagc acggaagcgt cgatgacgac gctcaccgcc ggtccgcatc cctggagcgc
     1201 aagatgtcgc gcgcagacac gcgcgacttg gcggcttcct tgcgccgccg aacgccgtcg
     1261 agccaggcgt cgatggacgg cctcgccgcc attctttcga gctcggagcg caggtactcc
     1321 tgcatcgact tgccttgacg agccgcgcgc gccgcgagct cgtcgcgcac gtgcgccgga
     1381 acatcgcgaa tcgtgatctg aacggtcatg caggcataat gccagcaacg gcagcacagt
     1441 gcaattagga gactgggcgt agtacgatgc tgcttcgcag atgacgtctt acttccagag
     1501 gtattcgaac gctcatgaac aagaagatct ggctcggctt gacgtgtagt tgccttgcgt
     1561 tctcggcaca ggcgcaattc ggcaccgagc ccgtcgagct cgagctgatc aaggtcaaag
     1621 acgacctcta cgtgatccac ggcgcctacg tccccggcaa cgtcacggcg ctgatcacca
     1681 acgagggcgt catcctcgtc gacgacgact tcgcggtcga tcacgacaac ctcatgaaga
     1741 agctgcgcga ggtcacgagg gagcccgtca agtacgtcat caacacgcac ttccacaacg
     1801 accacagcgg cggtaacgcg aagctgaagg ccgagggcac gctcgccgtc gcgtcgatcg
     1861 cggcgcgcac gcgcatgctg tcggccagcc cgcaggacgc cgccgacatc acggtcgagc
     1921 cgcgcggcgc cgtgcacttg ggcggcaagg tcgccgagat ctactacttc ggccgcggcc
     1981 acaccgacgg cgacgtcgtc gtattgttcc cgcacgaccg cacgctcgcc gccggcgact
     2041 tgttcacgca cggcgaaggc ctgccgcagc tcgtcgacca tgccggcggc ggcagcgcga
     2101 aggagtggac gggcacgctc gacaaggtcc tggcgctcga cttcgacacg gtcgtgccgg
     2161 ggcacggcac cgtcacgaac aagcaggcca tgcgcgacgc gcgcgcgagc tcgcagcgca
     2221 tgaccgaaat gctgacgcag atggtcaagc agaaccgctc gaaggccgac atcgaggcca
     2281 tgctgcgcaa cgagttcggc ttccaggact tccacatcat gatgtcgctc gacgggctca
     2341 tcaacgaaat gaaatgagcg cgtgagcggc tcgttgcacg gcgggggagg cgcgcagtga
     2401 aacgtccatc gttgctcgta gtcgccgtgc tcgcgctcgt cgtcgcgggg cttgcgtccg
     2461 gccagttccg cggccgccgc tcggcctaca ggtacggcac gttcgacgac aacacgccgc
     2521 cgcgcaccga gttcgtgaac atgcgctggc acttcggcac gaacggcgcg atcggccaca
     2581 tgggctggtc gcacaattac ccgtactccg agctcaactt gaacgagttc atcggccgca
     2641 cgacgcgcgt cgacgtcgag gacaactcgt atcgcggcat cgagctctcg agcccggaga
     2701 tcttccagta cccgttcgcg tacatctcgg agcccggcga gatggagatg accgaagccg
     2761 aggtcgcgaa cttgcgcgag tacatcgagc gcggcggctt catcctcatc gacgacttcg
     2821 acacctggca catggacaac ctgcgcgagg agatgcaccg cgtcttcccg gatcgcaatt
     2881 tcgagcggct gtcggtcgac aacccgatct tcaacttggt ccacgagatc gagagcctcg
     2941 acagcatggc gccctacgtc gcaggcgacg acccggtcta ctacggcctc gacaactcca
     3001 tggggcagat cgcgatcgtc gcgctgcaca acaacgactt cgcgaacttc tgggagtggt
     3061 acggcaaccc gggctatccg ctcgcgccgg cggccgacgc tttccgcatg ggcacgaact
     3121 tcatcgtgca cgcgatgacg cactgattcg gcttttggag ggctcacgat gacgaagcac
     3181 accacgacgc gccgcgactt cctcgctcgc tcgagcgcag cggcggcggc tgctgccagc
     3241 ttggcgcgca gcgcggcact cgcggccgtg gaagagcacg gcattccgca gcggccgatc
     3301 ccgagcacgg gcgagatgct gccggtcata ggactcggct cgagcaaagt cgtcgagcag
     3361 atcgcgcaga acggcacgga gccgctcctg ggcgtgctgc gcgcgctcgt cgcgcaaggg
     3421 ggcaagcttg tcgacacgtg gccgcgcaac gccgcaaacg acgcgggttt cggccgtgtg
     3481 gcctcgatgc cggagttccg cgacaagctg ttcgtgacga cgaaggtcga ccaagtcggc
     3541 aaggaagccg gcgtcaagca attccgcgat tcgctgcgca actaccagcg cagcgtcatc
     3601 gacctcgtgc agatcttcag cctgacggat ctcgacacgc actggccgag cttgaaggag
     3661 tggaaggccg agggcgcggc gcgctacatc ggcgtcacgg t
//
LOCUS       DASSNN010000516         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_90148, whole genome shotgun sequence.
ACCESSION   DASSNN010000516 DASSNN010000000
VERSION     DASSNN010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90148"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..740)
                     /locus_tag="VFL84_09610"
     CDS             complement(111..740)
                     /locus_tag="VFL84_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608921.1"
                     /translation="MLLRKLEQMLHELYALEVGYAVSDFLITDAELAGCLDFGGRKVD
                     EKLLIAEADGEADVALYLERELLDRLDRHDPLTKLDRDNLADFWAALEGVSHFTYYAW
                     NAARDKTVSLFELELQAEVDKFVTTGLLLREQTGREPNGLHGWLFDSPTLDAELKEHE
                     RERYQRANRYAAKYCQRLALARDDEAAHRELRRFYRLSQASKLAHIDSD"
     gene            complement(745..1368)
                     /locus_tag="VFL84_09615"
     CDS             complement(745..1368)
                     /locus_tag="VFL84_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608922.1"
                     /translation="MRTPTDRVHAVTSLQEFFKDSVADAMAKQGVAAEDHTAYYVVNL
                     LTLFARYETLYHEGNPGPGLQPVALLLAAAAETPNREARNALLRRIGDTSLFVAGFLG
                     DGFARKLIDIDYYIDMGGAAYGRLSDSVRGTREGRAFGTVFAELAEKFRDFVDVLAEI
                     RDSGRAAAIDVLRLYEVWLRTGSRRAARLLREHGLEPNATLNVATRH"
     gene            complement(1472..1906)
                     /locus_tag="VFL84_09620"
     CDS             complement(1472..1906)
                     /locus_tag="VFL84_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608923.1"
                     /translation="MRKQRHEIEVGCALTDAFTALVDVVAKGRWGGSAPVLDTALPIP
                     GRRYEAQRGKVLRRGKVLACLKPVSITLQETLLDPPCCVSLRLHWRIEPKDDGACVLL
                     DARYSLNGAAALRRRHWYERIHGHCVRMLGALAARVAECEET"
     gene            complement(2000..>2561)
                     /locus_tag="VFL84_09625"
     CDS             complement(2000..>2561)
                     /locus_tag="VFL84_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7608924.1"
                     /translation="VASGDWILLYAAAGGVGLIASQWARELGARVIGIVGTPAKRELA
                     LRFGCEHVLLDGDDIPRRVRELSGGGVPVVYDSVGRTTFYQSLDCLRPHGVLVSFGNS
                     SGKVEPFGLHELMRRGSLYVTRPTLQDFIGERASLEAGAGELFALVEKGRIEIEVNQS
                     YALRDAAQAHRDLEARKTTGSTVLIP"
BASE COUNT          434 a          858 c          856 g          413 t
ORIGIN      
        1 cctcccgaga ccgtgtgcgc acagggatgt gcgcgcacga gccgcaggga cgctcttccg
       61 gcgtgtctcg ggagggcctc tcgacggcgg tgcccggcga aaccgactgc ctaatcggag
      121 tcgatatgcg cgagcttcga cgcttgcgag agccggtaga aacgccgcag ctcgcggtgc
      181 gccgcttcgt cgtcgcgcgc gagcgcgagc cgctggcagt acttcgcggc atagcggttg
      241 gcgcgctggt agcgctcacg ctcgtgctct ttgagctccg cgtccaacgt cggcgaatcg
      301 aacagccagc cgtgcaagcc gttaggctcg cgccccgtct gctcgcgcag cagcaagccc
      361 gtcgtcacga atttgtcgac ctcggcttgc agctcgagct cgaacagcga gaccgtcttg
      421 tcgcgcgccg cgttccacgc gtaataggtg aagtggctga ccccttcgag cgcggcccag
      481 aaatccgcga ggttgtcgcg gtcgagcttc gtcagcggat cgtgcctgtc gagccggtcg
      541 agcagctcgc gctcgagata cagcgcgacg tcggcctcgc cgtcggcttc ggcgatcaag
      601 agcttctcgt cgaccttgcg cccgccgaag tcgaggcatc ccgcgagctc ggcgtccgtg
      661 atcaagaaat cgctgacggc gtagccgacc tcgagcgcat agagctcgtg caacatttgc
      721 tcgagcttgc ggagcagcat ctcgctagtg gcgcgtcgcg acgttcagcg tcgcgttcgg
      781 ctcgaggccg tgctcgcgca acaggcgcgc cgcgcgccgg ctgccggtgc ggagccagac
      841 ctcgtagagg cgcagcacgt cgatcgcggc ggcgcggccc gagtcgcgga tctcggcgag
      901 cacgtcgacg aagtcgcgga acttctcggc gagctcggcg aacacggtcc cgaatgcacg
      961 tccctcgcgc gtgccgcgca cgctgtcgga cagccggccg tatgcggcgc cgcccatgtc
     1021 gatgtagtag tcgatgtcga tgagcttgcg agcgaagccg tcgcccaaga agcccgcgac
     1081 gaacaacgac gtgtcgccga tgcggcgcag cagcgcgttg cgcgcttcgc gattcggagt
     1141 ctccgcggcc gcggccaaga gcagcgcgac gggttggagc ccggggccgg gattgccttc
     1201 gtggtacagc gtctcgtacc gagcgaatag cgtcagtagg ttgacgacgt agtaagcggt
     1261 gtgatcctcg gcagcgacgc cttgcttcgc catggcgtcg gcgacggagt ccttgaaaaa
     1321 ttcttgcagg ctggtgacag cgtgtactcg gtccgtcggc gtgcgcaaac aggtctcctg
     1381 caacctcggc gttgcactgc cgcacacgac ggcagagcgt gccgcttgct ttcgagtata
     1441 gagtcacgcc gcacggaaac tgcaaacggc ttcacgtttc ttcgcactct gcgacgcgcg
     1501 ctgccaaagc tccgagcatg cgcacacagt gaccgtgaat tcgttcatac cagtgtcggc
     1561 gtcgcaacgc cgcggcaccg ttcagcgagt agcgcgcatc gagcagcacg cacgcgccgt
     1621 cgtcctttgg ctcgatacgc caatgcaggc gcaggctcac gcagcacggc ggatcgagca
     1681 gcgtttcctg caacgtgatc gatacaggtt tgagacatgc aaggaccttg ccgcgccgca
     1741 gcaccttgcc gcgttgcgct tcgtagcggc gcccgggaat cgggagcgcc gtgtcgagaa
     1801 ccggcgcgct gccgccccag cggcctttcg cgacgacgtc gacgagcgcc gtgaatgcgt
     1861 ccgtcaacgc acagccgacc tcgatctcgt ggcgttgctt gcgcactgtc gtcctccgtg
     1921 acgagcgtca gcgtcgaagc taacgtggcg ccacgaggat cgcgacgcgc gaatccggcc
     1981 gaaacgcggc gaaattgcgc taggggatca gaaccgtcga gcccgtcgtc ttgcgcgcct
     2041 cgagatcgcg atgcgcttgc gcggcgtcgc gcaacgcgta gctctggttg acctcgatct
     2101 cgatgcgccc tttctcgacg agtgcgaaca gctcgccggc gcccgcttcg agtgacgcgc
     2161 gctcgccgat gaaatcctgg agcgtgggcc gcgtcacgta caacgagccg cgccgcatca
     2221 gctcgtgcaa accgaacggc tcgaccttgc cggacgagtt accgaagctg acgagaacgc
     2281 cgtgcggccg caagcaatcg agcgactgat agaacgtcgt gcggccgacc gagtcgtaga
     2341 cgacgggaac accgccgccc gagagctcgc ggacgcggcg cgggatgtcg tcgccgtcga
     2401 gcagcacgtg ctcgcagccg aagcgcaaag cgagctcacg cttcgcgggc gtgccgacga
     2461 tgccgatgac gcgcgcgccg agctcgcgcg cccactgcga ggcgatgagg ccgacaccgc
     2521 cggccgccgc gtacaagaga atccagtctc ccgaggcgac c
//
LOCUS       DASSNN010000517         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_323799, whole genome shotgun sequence.
ACCESSION   DASSNN010000517 DASSNN010000000
VERSION     DASSNN010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323799"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..914)
                     /gene="prmC"
                     /locus_tag="VFL84_09630"
     CDS             complement(66..914)
                     /gene="prmC"
                     /locus_tag="VFL84_09630"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET7608925.1"
                     /translation="MAKDSVLRTGDALTDAVKALTAVSETPRLDAELLLAFATRRTRS
                     AVIAFHERALDPAAAKLFSALLERRARGEPLAYLTQQREFFSLPLVVSPAVLIPRAET
                     ELLVELTLAAIAPAARPAVLDVGTGSGAIALAIKRERGDALVTATDRSAAALAVARRN
                     AAHLALDVRFVESDWLEKLGGEMFDVIASNPPYVRSAEIVGSLTFEPRVALDGGADGL
                     DAYRVLFAAARDHLNAGGALLVEHGAQQRAELVALAAANGWPVAAAHDDLAGRPRVLE
                     LARGAP"
     gene            complement(934..2022)
                     /gene="prfA"
                     /locus_tag="VFL84_09635"
     CDS             complement(934..2022)
                     /gene="prfA"
                     /locus_tag="VFL84_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET7608926.1"
                     /translation="MKESLRTKLTELVERHEEVARLLGDAATISDKDRFRELSKEYAR
                     LDEVARDFNLHQELEQHIAAAAELRESADPDMRALAEDEHKELSGRLAALERQLLVHL
                     VPKDPHDDANLFLEVRAGTGGDEAAIFAGDLFRMYSRYAERQGWSVEVLSSNAGEHGG
                     YREVISRVAGKGAYSKLKFESGVHRVQRVPETEAQGRIHTSTCTVAVFPEPEDIGEIE
                     VNKADLKIDTYRASGAGGQHVNKTDSAIRITHLPTGIVVECQDERSQHKNRARAMSLL
                     QARLQDAEETKRQTETAEKRRSLVGTGDRSERIRTYNFPQGRLTDHRINLTLYKLEDI
                     VNGDLDEVIGPLANEYQADQLGALGLEA"
     gene            complement(2019..>2460)
                     /locus_tag="VFL84_09640"
     CDS             complement(2019..>2460)
                     /locus_tag="VFL84_09640"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET7608927.1"
                     /translation="GVSGTVETIGIFNTVLKTPDNRVLHVPNSLVYAGTITNYNAEST
                     RRIDMTIAIGYDADIPQAKSVIAAVVAAESRVAPNPAPEVAVLEVYPAFVNVAVRVWA
                     QSGDYGSVRSDLLERIKRSLDKYGLSIPAEQRAMPPPAQLTASK"
BASE COUNT          350 a          859 c          837 g          414 t
ORIGIN      
        1 cccgagcttc gcctgccctt ccgcgccgac ctcggccagc gcgatctgcc gcgcgtagcg
       61 ccggctcatg gcgcgcctct cgcgagctcg agcacgcgcg gccgtcccgc gagatcgtcg
      121 tgcgcggccg cgacaggcca gccgttcgcc gctgcgagcg cgaccagctc ggcgcgttgc
      181 tgcgcgccgt gctcgacgag caacgcgccg ccggcgttca aatgatcccg ggccgcggcg
      241 aacagcacgc gatacgcgtc gaggccgtcg gcgccgccgt cgagcgcgac tcgcggctcg
      301 aacgtcaacg agccgacgat ctcggccgaa cgcacgtagg gcgggttgct cgcgatgacg
      361 tcgaacatct cgccgccgag cttctcgagc caatccgatt cgacgaagcg aacgtcgagc
      421 gcaaggtgcg ccgcgttgcg gcgcgcgacg gccaacgccg cggcgcttcg gtccgtcgcc
      481 gtcacgagcg cgtcgccgcg ctcgcgcttg atcgccaatg cgatcgcgcc gcttcccgtg
      541 ccgacgtcga gcacggccgg ccgagcggcc ggcgcgatag cggcgagcgt gagctcgacc
      601 agcagctccg tctcggcacg gggtatcaac acggccggcg atacgacgag cggcaacgag
      661 aagaactcgc gttgctgcgt caggtacgcg agcggctcac cgcgcgcgcg ccgctcgagc
      721 agcgccgaga acagcttcgc agccgccgga tcgagcgcgc gctcgtgaaa ggcgatcacg
      781 gcgctgcgcg tccgccgcgt cgcgaacgca agcaacagct ccgcatcgag gcgcggcgtc
      841 tcgctgaccg ccgtgagcgc tttcacggcg tcggtcagcg cgtcaccggt tcgcaagacg
      901 gaatcttttg ccatcgcacg tgcgctcgtc atctcaggcc tcgagcccga gcgcgccgag
      961 ctggtcggct tggtactcgt tcgcgagcgg cccgatcacc tcgtcgaggt cgccgttgac
     1021 gatgtcctcg agcttgtaca gcgtcaggtt gatgcggtga tccgtgagcc ggccctgcgg
     1081 aaagttgtag gtgcggatgc gctccgagcg gtcgccggtt ccaacgaggc tgcggcgctt
     1141 ctcggccgtc tcggtctggc gcttcgtctc ctcggcgtcc tgcaagcgcg cttgtaacag
     1201 cgacatcgcg cgcgcgcggt tcttgtgctg cgagcgctcg tcttggcact cgacgacgat
     1261 gccggtcggc aagtgcgtga tgcggatcgc cgagtcggtc ttgttgacgt gctgaccgcc
     1321 ggcgcccgac gcgcggtacg tgtcgatctt caagtccgct ttgttgacct cgatctcgcc
     1381 gatgtcctcg ggctcgggga acacggcgac cgtgcacgtc gacgtgtgga tgcggccttg
     1441 cgcctcggtc tcgggaaccc gctgcacgcg atgcacgccg gactcgaact tcagcttcga
     1501 gtacgcgccc ttgcctgcga cgcgcgagat gacctcgcga tagccgccgt gctcgccggc
     1561 attggagctc agaacctcga cgctccaccc ttgccgctcg gcatagcggc tgtacatgcg
     1621 gaacaagtcg ccggcgaaga tcgcggcttc gtcgccgccg gtgcccgcgc gcacctcgag
     1681 gaacagattc gcgtcgtcat gcggatcctt cggcacgagg tgcacgagca actggcgctc
     1741 gagcgccgct agccgtccgg aaagctcctt gtgctcgtcc tcggcgagcg cgcgcatgtc
     1801 gggatcggcg ctctcgcgca actccgcggc ggccgcgatg tgctgctcga gctcttgatg
     1861 caaattgaaa tcgcgcgcga cctcgtcgag gcgcgcgtac tccttcgaga gctcgcggaa
     1921 ccgatccttg tcggagatcg tcgccgcgtc accgagcagc cgggcgacct cttcgtgccg
     1981 ctcgaccaac tccgtgagct tggtgcgcag cgactccttc acttgctcgc cgtcagctgg
     2041 gccggcggcg gcatggcgcg ttgctcggcc gggatcgaca ggccgtactt gtcgagcgag
     2101 cgcttgatgc gctcgagcag gtcgctgcgc acggagccgt agtcgccgct ctgcgcccac
     2161 acgcgcacgg cgacgttgac gaaggcggga tacacctcga ggacggcaac ctccggcgcg
     2221 gggttcggag cgacgcgcga ctccgccgcg acgacggccg cgatgaccga cttcgcctgc
     2281 gggatgtcgg cgtcgtagcc gatcgcgatc gtcatgtcga ttcggcgcgt gctttcggcg
     2341 ttgtagttcg tgatcgtgcc ggcgtacacg agactgttcg gcacgtgcaa gacgcggtta
     2401 tcgggcgtct tcagcaccgt gttgaagatg ccgatcgtct cgaccgtgcc gctgacgccc
//
LOCUS       DASSNN010000518         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_90393, whole genome shotgun sequence.
ACCESSION   DASSNN010000518 DASSNN010000000
VERSION     DASSNN010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90393"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFL84_09645"
     CDS             complement(<1..812)
                     /locus_tag="VFL84_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006079914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HET7608928.1"
                     /translation="MREVNYTPPSSSSVFRLGLSVLVGIVIAYLIFGSWYQVDQGERA
                     VVLRFGELVGESGAGLHFKLPWVDAVRKITVQNQTKRYQSLEAYSRDQQPADLTVSVT
                     YVVVNPSEVYAQYGDLEGAVLRLIDPRLTAGIKTIFGQYDAVRAIQERTALNSDFDTA
                     ITSAIDGGPINVISVQIENIDFSDAYEQSVEQRMLAQVEIQRREQNLRTTEVEAQIAK
                     TKAEGEAEAAAIRARADALRANADLVQLQAVEKWDGKLPTTMVPSSALPFIN"
     gene            complement(917..>2121)
                     /locus_tag="VFL84_09650"
     CDS             complement(917..>2121)
                     /locus_tag="VFL84_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608929.1"
                     /translation="LPEPTQALEESAPPDSIVTPEPEAPIAADSPAELSAAQGELAAP
                     SEEASSVAKAASVSAAEQAPVGNADIQEMAAAQFSDSTIIAVIDANEVEFDLSPRALV
                     ALKNAGVSEPVIEAMLAAQAGEGQEAEPELAAAQSEAQQSAEFAKLTEMIEQLAARQE
                     AAEKARRAPEPPVSRADPTPRVWLVEDGERAAVAPTIAQVAVTGGSGKRGERMKNLQG
                     LANQALAFANPAVSGIATTLGSLFHPNEERTAVWALASPQSARVLDTAPVFEMDFAHI
                     PGVDPAKYQPAIVQLVPTSDNYRLVAAAETDGANARAMPRGPIIEETVETQSTRLARG
                     RYRIAPKGELGPGEYALVLRPVPETGRRRGRDKQASLGELLGGSTSQILYLTWEFSLA
                     GQPVANAL"
BASE COUNT          316 a          709 c          707 g          389 t
ORIGIN      
        1 aagttgatga acggcagagc ggagctcggc accatcgtcg tcggcagctt gccgtcccac
       61 ttttcgacgg cttgcagctg cacgaggtcg gcgttcgctc gcagcgcgtc ggcgcgcgcg
      121 cgaatcgccg cggcctcggc ctcgccttcg gccttggtct tcgcgatctg cgcttcgact
      181 tccgtcgtgc gcaaattctg ctcgcgccgt tgaatctcga cttgcgcgag catgcgctgc
      241 tcgacgctct gctcgtacgc gtccgaaaag tcgatgttct cgatttgcac gctgatgacg
      301 ttgatcggac cgccgtcgat cgcggaagtg atggccgtgt cgaagtcgct gttcaaagcg
      361 gtacgctcct gaatcgcgcg cacggcgtca tactggccga agatcgtctt gattccagcg
      421 gtgagccgcg gatcgatgag ccgcaggacg gcgccctcga gatcgccgta ttgcgcgtag
      481 acctcgcttg gattcacgac cacgtacgtg acgctgactg tgagatcggc gggctgctga
      541 tcgcgcgaat aggcctcgag gctctggtag cgcttcgttt ggttctgcac ggtgatcttg
      601 cgcaccgcgt cgacccacgg aagcttgaag tggagacccg cacccgattc gccgacgagc
      661 tcgccgaatc gcaaaacgac ggcgcgctcg ccttggtcga cttgatacca ggaaccgaat
      721 atcaagtaag caatcacgat accgacgagc accgaaagtc ccagacggaa cacgctgctc
      781 gagcttggcg gcgtgtaatt gacttccctc acggcttagc cctcttgttg tttagttgcg
      841 aggccgtaaa cgcggaaatg agagcgcgaa gagcatacca cgcgcgagct gcggaaccgg
      901 ccgtgcgggg ggcgcgctag agagcgttcg cgacgggctg gccggcgagc gagaattccc
      961 acgtcaggta caggatctgg ctcgtgctgc cgccgagcag ctcgccgaga gacgcttgct
     1021 tgtcgcgccc gcggcggcgg ccggtctcgg gaacgggacg cagcacgagc gcgtattcgc
     1081 cggggccgag ctcgcccttc ggcgcgattc gatagcggcc gcgggccaag cgcgtgctct
     1141 gcgtctcgac tgtctcctcg atgatcggcc cgcgcggcat cgcgcgcgcg ttggcaccgt
     1201 cggtttccgc cgcggcgacg agacggtagt tgtcgctcgt cggcacgagc tgaacgatcg
     1261 cgggctgata tttcgcggga tcgacgccgg ggatatgcgc aaagtccatc tcgaacacgg
     1321 gcgccgtgtc cagcacgcgc gcgctctgcg gcgacgcgag cgcccagacg gccgtgcgct
     1381 cttcgttggg gtgaaacaag ctgccgagcg tcgtcgcgat gccgctgacg gcggggttcg
     1441 cgaacgcgag cgcctggttc gcgagccctt gcaaattctt catgcgctcg cctcgcttgc
     1501 cgctgccgcc cgtgaccgcg acttgcgcga tcgtcggggc gaccgccgct cgctcgccgt
     1561 cctcgaccag ccagacgcgc ggcgtcggat ctgcacgcga cacgggcggc tccggcgcgc
     1621 ggcgcgcctt ctcggccgct tcctgccgcg cagccagctg ctcgatcatc tccgtgagct
     1681 tggcgaattc ggcggactgc tgcgcctcgg attgcgccgc cgcgagctcc ggctctgcct
     1741 cctgcccctc gcccgcctgc gccgccagca tcgcttcgat gacgggctcg gacacgcccg
     1801 cgttcttcag cgcgacgagc gcacgcggcg acagatcgaa ctcgacttcg ttcgcatcga
     1861 tgacggcgat gatcgtcgaa tcgctgaatt gcgccgcagc catctcttgg atgtccgcgt
     1921 tcccgaccgg ggcttgctcg gcggcgctga ctgacgcggc cttggcgacc gatgacgctt
     1981 cctcgctcgg tgcggcgagc tcaccttgtg cagcggataa ttccgcgggc gagtcggccg
     2041 cgatcggcgc ctcgggctcg ggcgtaacga tcgagtccgg cggcgcagat tcctcgagcg
     2101 cctgcgtcgg ctcgggcagc g
//
LOCUS       DASSNN010000519         3126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1102626, whole genome shotgun sequence.
ACCESSION   DASSNN010000519 DASSNN010000000
VERSION     DASSNN010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102626"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1643
                     /gene="uvrC"
                     /locus_tag="VFL84_09655"
     CDS             <1..1643
                     /gene="uvrC"
                     /locus_tag="VFL84_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET7608930.1"
                     /translation="VRAIAGIEVTVTRTEVEALMLEYNLIKEHRPRFNVMLRDDKSYP
                     YILLETGHRFPRLSFYRGPRTSKGRLFGPYPNAGSVRESLNQLHKLFQLRQCEDSYFE
                     NRSRPCMQHQIERCTAPCVGLISEEDYARDLEHAVLFLEGHSDVVTARLAERMEQAST
                     ALQFERAAQYRDQLARLKNVQSQQLMARSSGDFDAVGMAEDHGIYCVAVMFFRGGRSL
                     GTRSFFPKLVKGADDDEIIRAFLLQYYGGREAPREILVSHAVPEAAALAELLSAQSNH
                     KVAIKARVRGDRARFVEMAVTNARHSAELRYQSSASYERQLEELCEVLELDEPPTRLE
                     CFDVSHTAGEATVASCVVFGPEGPLKSDYRRFNVADVTPGDDYGAMAQVLSRRYARVQ
                     KGEVPMPDVLFIDGGPGQLARAIAVLQELGVSGARLVGVAKGQDRKPGRETLYLADRT
                     DPLRLPPSSPALHLIQQLRDEAHRFAITGHRARRQKARTRSPLEDIPGLGPKKRRELL
                     KQFGGLQALTRAGIDDLQRVRGISRQLAEAIYERFHAG"
     gene            1657..2235
                     /gene="pgsA"
                     /locus_tag="VFL84_09660"
     CDS             1657..2235
                     /gene="pgsA"
                     /locus_tag="VFL84_09660"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET7608931.1"
                     /translation="MKFNLPNALTWLRVVAIPLVVAVFYGPELFGWHESWTRPATGLL
                     FGLAGITDYFDGYLARRLGLTSSFGAFLDPVADKLIVSTALVLLVQADPQITLALVAA
                     IIIGREITVSALREWMSQIGARAHVAVSIFGKWKTTLQIIGIGLMLYRETLVGLPTYR
                     IGEWLLYVAAALTLWSMIDYLRAAWPAMRERS"
     gene            2304..2379
                     /locus_tag="VFL84_09665"
     tRNA            2304..2379
                     /locus_tag="VFL84_09665"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2337..2339,aa:Gly,seq:gcc)
     gene            complement(2395..2679)
                     /locus_tag="VFL84_09670"
     CDS             complement(2395..2679)
                     /locus_tag="VFL84_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608932.1"
                     /translation="MPGVIDLDCGAYDVKAGSIGHGQCGYVFGCTISPVPEDLSSYLM
                     MSARLGATIRLVFPEQPLLLERVEIERVDVVSVRIVGNVIDGATKRGLPS"
     gene            complement(2749..>3126)
                     /locus_tag="VFL84_09675"
     CDS             complement(2749..>3126)
                     /locus_tag="VFL84_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608933.1"
                     /translation="AGDDAATPLAKLPTVGYVWPSGSPVGYSIKYTDRATQPDGGERI
                     TFVTDKRLGSYDFKGWSVASPGSGADAAYSVIELDLNGSGTGTGTLSLVGEVVVDEQA
                     GTVALKPGAPSLLTNVKREATTH"
BASE COUNT          512 a         1034 c         1044 g          536 t
ORIGIN      
        1 tggtgcgcgc gatcgccgga atagaagtga ccgtcacgcg caccgaagtc gaagcgctga
       61 tgctcgagta caacctcatc aaggagcacc ggccgcgctt caacgtcatg ctgcgcgacg
      121 acaagagcta tccgtacatc ctgctcgaga cgggccatcg cttcccgcgg ctgtctttct
      181 accgcggccc gcgcacgtcg aaggggcggc tgttcggccc ttacccgaac gcgggctcgg
      241 tgcgcgaatc gctgaatcag ctccacaagc tgttccaact caggcaatgc gaggacagct
      301 acttcgagaa ccgcagccgc ccgtgcatgc agcatcagat cgagcgctgc acggcgccgt
      361 gcgtcggcct catcagcgag gaggactacg cgcgcgatct cgagcacgcc gtgctgttcc
      421 tcgagggcca cagcgacgtc gtcacggcgc ggctcgccga acgcatggag caggcgtcca
      481 cggcgcttca gttcgagcgg gcggcgcagt accgcgatca gctcgcgcgg ctcaagaacg
      541 tgcagtcgca gcagctcatg gcgcgctcga gcggcgactt cgacgcggtc ggaatggccg
      601 aagaccacgg catctattgc gtcgccgtga tgttcttccg cggcggccgc tcgctcggga
      661 cgcgcagctt ctttccgaag ctcgtcaaag gcgccgacga cgacgagatc attcgcgcgt
      721 tcctgctcca gtactacggc ggccgcgaag cgccgcggga aattctcgtg agccacgcgg
      781 taccggaggc cgccgcgctc gccgagctct tgtccgcgca gtcgaaccac aaggtcgcga
      841 tcaaggcgcg cgtgcgcggc gaccgggcgc ggttcgtcga gatggccgtc acgaacgcgc
      901 ggcacagcgc cgagctgcgg tatcaatcga gcgcgtccta cgagcgccag ctcgaggagc
      961 tttgcgaggt gctcgagctc gacgagccgc cgacgcggct cgagtgcttt gacgtcagtc
     1021 acacggccgg ggaagctacc gtcgcgtcgt gcgtcgtatt cgggccggaa gggccgctca
     1081 agtccgatta tcggcgcttc aacgtcgccg acgtgacgcc cggcgacgac tacggcgcga
     1141 tggcgcaagt gctgtcgcgc cgctatgcgc gcgtgcagaa aggcgaggtg ccgatgccgg
     1201 acgtgttgtt catcgacggc ggcccggggc agctcgcgcg cgcgatcgcc gtgctgcagg
     1261 agctcggcgt ctcgggcgcg cggctcgtcg gtgtcgccaa aggccaggac cgcaagccgg
     1321 gccgcgaaac cttgtacttg gccgatcgca cggatccgct gcgtttgccg ccgagctctc
     1381 cggcattaca cttgattcag cagttgcgcg acgaggcgca tcgcttcgcg atcacgggcc
     1441 accgcgcgcg gcggcagaaa gcgcgcacgc gctcgccgct cgaggacatc ccggggctcg
     1501 gaccgaagaa gcgccgcgag ctgctgaagc agttcggcgg tttgcaggcg ctgacgcgcg
     1561 ccggcatcga cgacttgcag cgagtgaggg gcatatcgcg ccagctggcc gaggcgattt
     1621 acgaacggtt ccacgccggc tgacggttca cgacgcatga agttcaatct gccgaacgca
     1681 ctgacctggt tgcgcgtcgt cgcgattccg ctcgtcgtgg ccgttttcta tggccccgag
     1741 ctctttggct ggcacgagtc gtggacgcgg cccgcgacgg ggctcctgtt cggcctggcc
     1801 gggatcaccg actacttcga tggctatctc gcgcggcggc ttggtctcac gtcgagcttc
     1861 ggcgcgtttc tcgatcccgt cgccgacaag ctgatcgtct cgacggcgct cgtcctgctc
     1921 gtgcaggccg atccgcagat cacgctcgcg ctcgtcgcgg cgatcatcat cggccgcgag
     1981 atcacggtct cggcgctgcg cgaatggatg agccaaatcg gcgcgcgcgc gcacgtcgcg
     2041 gtcagtatct tcggcaagtg gaagacgacg ctgcagatca tcggcatcgg cctgatgctg
     2101 taccgcgaga cgttggtcgg attgccgacg tatcgaatcg gcgagtggct gctctatgtc
     2161 gcagccgcgc tgacgctgtg gtcgatgatc gactacttgc gcgccgcgtg gcccgcgatg
     2221 cgcgagcgct cctaggccgc ttccgcgacg agccgcatgc cgttgccggc cgtggcaacg
     2281 ggctttagaa tggcgccctc gacgcgggaa tagctcagtt ggtagagcgc aaccttgcca
     2341 aggttgaggt cgcgagttcg agcctcgttt cccgctccac tcatccaaga gggcctacga
     2401 cggtaggccc cttttcgttg cgccgtcgat cacgttgccg acgatccgaa cggagacgac
     2461 gtcgacgcgc tcgatctcga cgcgctcgag cagcaacggt tgctcgggaa acactagccg
     2521 aatcgtcgcg ccaagccggg ccgacatcat caagtacgac gagaggtcct ccgggacggg
     2581 gctgatcgtg cagccaaaca cgtagccgca ctgaccgtga ccgatcgaac cggccttgac
     2641 gtcgtaggct ccgcagtcga ggtcgatgac cccaggcatg tcgatctcca gtacaacgac
     2701 aggtacgggg tagggcggca aggccgccct accgtttttg tctcccagtc aatgtgtagt
     2761 ggcttcgcgc ttgacgttcg tcaacaagct gggtgcaccc ggtttcaggg cgaccgtgcc
     2821 ggcttgctcg tcgaccacga cttcgccgac gagcgatagc gtccccgtgc cggtccccga
     2881 gccgttcaag tcgagctcga tcacggagta ggccgcatcg gcgccgctgc cggggctcgc
     2941 cacggaccag cctttgaagt cgtagctgcc gaggcgtttg tcggtcacga acgtgatgcg
     3001 ctcgccgccg tcgggctgcg tggcgcgatc cgtgtacttg atcgaatagc cgaccgggct
     3061 gccgctcggc cagacatagc cgaccgtggg cagcttcgcg agcggcgtag ccgcgtcgtc
     3121 gccggc
//
LOCUS       DASSNN010000520         5505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_323892, whole genome shotgun sequence.
ACCESSION   DASSNN010000520 DASSNN010000000
VERSION     DASSNN010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323892"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFL84_09680"
     CDS             complement(<1..434)
                     /locus_tag="VFL84_09680"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HET7608934.1"
                     /translation="MLYRDGAADEALAERDWIVLVNYVAAGNQRALRQLFERTHRLVF
                     SLIARITNSREVAEDLTIEVFSDLWRRARSYDPAHGSVLGWIMGQARAKAMGWLQLDR
                     ELDTDVLTPTTPLWGAIAHRIVSEYGVQPTFTAPLDWTDPDW"
     gene            685..1230
                     /locus_tag="VFL84_09685"
     CDS             685..1230
                     /locus_tag="VFL84_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7608935.1"
                     /translation="MADERETAAAAPDPQVAFVTGVFVRFQRSLLRYLRDLLLRREDA
                     EDVAQETYLRLVRSAGADRSEMRIRALMFRAATNLAYDRFRQRRSRGPHDDAELAALR
                     DETPAAEHVVALEQALGVVERTVLGLPLRCRRVFLLRTSHECSYEEIAERLGVSKRTV
                     EREMQAALEACQRELRGGELP"
     gene            1227..2102
                     /locus_tag="VFL84_09690"
     CDS             1227..2102
                     /locus_tag="VFL84_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608936.1"
                     /translation="MTRTDSTGNEAGAGTAALFVSVDAGEQGADVALASWLAERPDHE
                     RELERVELAAVLGKRLAAEPGSALHGEAAAAARVRSRGWVGRASAWGGALAAAVLVTV
                     FLLPHEPPGPADPELAPLRAAERVSIDATSDAAVVLPTGVVVDLSAVAVLPFSTSGDP
                     TLAARLERGVAASLRTVPGLYVIADEAVRPYAATQLDTAEIGGQLGARGIVDGAVELV
                     DGRVHVSARLREAATGATLWQTGVDRPVAELGAVRSEIAESIAATMLDSSLREQAARA
                     SRSGAPVSVSKPIPQ"
     gene            2157..2828
                     /locus_tag="VFL84_09695"
     CDS             2157..2828
                     /locus_tag="VFL84_09695"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HET7608937.1"
                     /translation="MLVLIFGIGNDAAAQARQGQQDQAAEIERLRRELEQVRQQQQPG
                     QPLLVVPSMQPPPPPPRIDPEQPMVDLLPLLQRVERASGKRFIVDRRLGSRIYLGTVD
                     PDDVTYPALLAILRINGFAAFESEGFVNIVPDANIRFHETRIVQTDDASIPAEEFVTR
                     VLAPKNIQAAMLVPILRPLMPQPAHLAAHSDSNQLILMDRYANVQRITAIVRSLDVPS
                     ARQGQ"
     gene            2868..3983
                     /locus_tag="VFL84_09700"
     CDS             2868..3983
                     /locus_tag="VFL84_09700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7608938.1"
                     /translation="MRIAHVEAGRHLYGGAAQVNYLLEGLTGRGVENLLFCPKDCELG
                     AAATSALVRALPMRGELDATLLPRLAAALKRAKPDLVHVHSRRGADLYGGLAAALAGV
                     PAVVTRRVDAAEPALLARLKYRSYRAVVALSHAIERQLVAAGVARERIVLIPSGIDTE
                     RYRPDPAARERLRAAFALRADALVVGVVAQLIERKRHSWLFASLPELLREWPQLAVVC
                     FGRGPLEGGLLVELAERGLTGRVVLAGFRSDLPALVPGLDVLAHPAGREGLGLALLEA
                     ASAGVPVVACAVGGVPDAVVDGETGLLVGRDDASGMTRALRSLLGSPETRARFGAAAR
                     RHVERRFDVSKLVGAHHSLYTRVLGERAAAAAPVGLE"
     gene            4016..4489
                     /locus_tag="VFL84_09705"
     CDS             4016..4489
                     /locus_tag="VFL84_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HET7608939.1"
                     /translation="MSLGRALKARGLRVATAESCTGGWIAKALTDVAGSSQWFEGGVV
                     AYSNAAKISLLGVASGVLAANGAVSEETVRAMAEGARSRFAADFAVAVSGIAGPDGGS
                     ADKPVGTVHFAWAAPGGTTAARRIFAGDRETVRRLTVALALERLIELATVDERPP"
     gene            4473..5006
                     /gene="thpR"
                     /locus_tag="VFL84_09710"
     CDS             4473..5006
                     /gene="thpR"
                     /locus_tag="VFL84_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET7608940.1"
                     /translation="MSDPLNERPDRHRVFFALWPDDATRAAISRSTREAVRLSGGRPI
                     AKERLHITVAFLGELTAAGVEAARAVPPIPVGPFELTLDAIGVWPESKILWLAPTALP
                     AALAELENLLWDGLVECGFRGAERDYLPHVTLARRARPVESAVEPVRFPVRDLALVES
                     FPDGRNVHYEVLERWDL"
     gene            5176..>5505
                     /locus_tag="VFL84_09715"
     CDS             5176..>5505
                     /locus_tag="VFL84_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006460571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HET7608941.1"
                     /translation="MDDNRKKALSAALGQIEKQFGKGSVMRLGDAIASRDIEVISTGS
                     LGLDLALGVGGLPRGRVIEIYGPESSGKTTLALHAIAEAQKNGGTAAFVDAEHALDPV
                     YAKKLGVD"
BASE COUNT          781 a         1899 c         1957 g          868 t
ORIGIN      
        1 ttccagtcgg ggtccgtcca atcgagcggc gccgtgaatg tcggctgcac gccgtactcc
       61 gagacgatgc ggtgcgcgat cgctccccag agcggcgtcg tcggcgtcag cacgtcggta
      121 tcgagctcgc ggtcgagctg caaccacccc atcgccttcg cgcgcgcctg gcccatgatc
      181 cagccgagca cggagccgtg ggccggatcg taggagcgcg cccggcgcca tagatcgctg
      241 aacacctcga tcgtgagatc ctcggcaacc tctcggctgt tcgtgatgcg cgcgatcaag
      301 ctgaacacca gcctgtgcgt acgctcgaag agctggcgca gcgcccgctg attgccggcc
      361 gcgacgtagt tgacgagcac tatccagtcg cgctcggcca gcgcctcgtc ggcggcgccg
      421 tcgcggtata gcacgtcccc cagcgttgcc gccggcggcg ggacggcgct gaataggcca
      481 tcgtcgtttc gtgaggtcac gggtttggga gggaggatcc ctgaatccat ctggatttta
      541 gaggcaagtt tggtgccagg aaacccgtct aattgcagcg tgcacgcaac atccgtggca
      601 gacgcgtggc ggaaagccgg gcctcggccg tattaggact gtgtgagcat cagggtcgta
      661 cgaccgagcg aggcacgtcc ttcgatggcc gacgagcgcg agactgcggc ggccgcaccc
      721 gatccgcagg tggcgttcgt gaccggcgtc ttcgtgcgtt tccagcgttc gctgctgcgg
      781 tatctgcgcg acctgctgct tcgccgcgag gacgccgagg acgtcgcgca ggaaacctat
      841 ctcaggctcg tgcggtccgc aggggccgac cgctcggaga tgcggattcg cgcgctgatg
      901 ttccgggccg cgacgaacct ggcctacgac cggttcaggc agcggcgatc gcggggtccg
      961 cacgatgacg ccgagctcgc ggcgctgcgc gacgagacgc cggccgccga gcacgtcgtc
     1021 gcgctcgagc aggcgctcgg cgtcgtcgag cgcaccgtgc tcggcttgcc gctgcgctgc
     1081 cgccgcgtat tcctgctgcg aacgtcgcat gagtgcagct acgaggagat cgccgagcgg
     1141 ctcggcgtca gcaagcgcac cgtcgagcgc gagatgcaag ccgcgctcga ggcgtgccag
     1201 cgagagttac gaggaggaga gttgccatga cgcgaacgga ttcgactggc aacgaggcgg
     1261 gggcaggcac ggccgccctg ttcgtgagcg tcgacgccgg cgagcagggc gccgacgtcg
     1321 cgctcgcgag ctggctcgcc gaacggcccg atcacgaacg cgagctcgag cgcgtcgagc
     1381 tcgccgcagt gctcggcaag cggctcgcgg ccgagcccgg cagcgcgctg cacggcgagg
     1441 ctgccgcggc cgcgcgcgtt cgatcgcggg ggtgggtggg ccgcgcgtcg gcgtggggcg
     1501 gcgcgttggc ggccgcggtg ctcgttaccg tgttcctgtt gccccatgag ccgccggggc
     1561 cggccgaccc cgagctcgcg ccgttgcgcg ccgccgagcg ggtgagcatc gacgctacga
     1621 gcgatgccgc ggtcgtgctg ccgaccgggg tcgtcgtcga cttgagcgcc gtggcagtgc
     1681 tgccgttctc gacgtcgggc gacccgacgc tggccgcgcg cctcgagcgc ggcgtcgcgg
     1741 cctcgctgcg cacggttccg ggcctgtacg tgatcgcgga cgaagccgtc cggccctatg
     1801 cggccacgca gctcgacacc gccgagatcg gcggccagct cggcgcgcgc ggcatcgtcg
     1861 acggcgccgt ggagctcgtc gacggccgcg tgcacgtgtc cgcgcgcctg cgcgaggccg
     1921 cgaccggagc gacgttgtgg cagaccggcg tcgaccgccc cgtcgccgag cttggcgccg
     1981 ttcgctccga gatcgccgag agcatcgccg caacgatgct cgactcgagc ttgcgagagc
     2041 aggccgcgcg tgcgagccga tccggcgcgc ccgtttcggt cagtaaaccg atcccgcaat
     2101 gacgttccac gaaggagcct taaatgcgat ccaatcgatt tctttgtgct ttcgcaatgc
     2161 tcgtgttgat ttttggaatc ggcaacgacg cagcggccca ggcccggcaa gggcagcaag
     2221 atcaagctgc agagatcgag cgcttgcgcc gcgagctcga gcaggtgcgg cagcagcagc
     2281 aaccggggca gccgctgctc gtcgtcccgt cgatgcagcc gccgccaccg ccacccagaa
     2341 tcgacccgga gcagccgatg gtcgatctgc tgccgctgct gcaacgcgtg gaacgcgcct
     2401 cgggcaagcg gtttatcgtc gatcgcaggc tcggctcgag gatctacttg gggaccgtcg
     2461 atccggacga cgtgacgtac ccggcgctgc tcgcgatcct caggatcaac ggattcgcgg
     2521 ccttcgagag cgagggcttc gtcaatatcg ttccggacgc caacattcgc tttcacgaga
     2581 cgcgtatcgt gcaaaccgat gacgcgtcga ttccggccga agagttcgtc acgcgcgtgc
     2641 tcgcgccgaa gaacatacag gccgctatgc tcgtgccgat attgcggccg ctgatgccgc
     2701 agccggccca cctggcggcg cactcggact cgaaccagct catcctcatg gaccgctatg
     2761 ccaatgttca gcgaatcacc gcgatcgtcc gctcgctcga cgtgccgtcg gcgcggcagg
     2821 gtcagtagcg agcgaccagg ggccacggtg ccgcgtatga tcgaggcatg cgaatcgcgc
     2881 acgtcgaggc cggccggcat ctctacggcg gcgcggccca ggtcaattac ctgctcgaag
     2941 gactgacggg ccgcggcgtc gagaacctgc tcttctgccc gaaggattgc gagctcggcg
     3001 ccgcggctac gtcggcgctc gtgcgcgcgt tgccgatgcg cggcgagctc gacgcgacgc
     3061 tgctgccgcg cctcgccgcc gcgctgaagc gcgcgaagcc cgacctcgtg cacgtccact
     3121 cgcgccgcgg cgccgatctt tacggcggtc tcgccgcggc gctggccggc gtccccgcgg
     3181 tcgtcacgcg gcgcgtcgac gcggcggagc cggctctgct cgcgcggctg aagtatcgct
     3241 cctatcgcgc cgtcgtggcg ctgtcgcacg cgatcgagcg gcagctcgtc gcggccgggg
     3301 tcgcgcgcga gcgcatcgtg ctgatcccga gcggcatcga taccgagcgc tatcgccccg
     3361 acccggcggc gcgcgagcgg ctgcgcgccg ccttcgcgtt gcgcgccgac gcgctcgtcg
     3421 tcggcgtcgt cgcgcagctg atcgagcgca agcgccattc gtggctgttc gcgtcgttgc
     3481 ccgagctgct gcgcgaatgg ccgcagctcg cggtcgtctg cttcggccgt gggccgctcg
     3541 agggagggct gctcgtcgag ctcgcggaac gcgggttgac gggccgcgtc gtgctcgcgg
     3601 gctttcgctc ggacttgccg gcgctcgtgc cgggcctcga tgtgctcgct catccggccg
     3661 gccgcgaggg tcttgggctc gcgctgctcg aagcggcgag cgcgggcgtt cccgtcgtcg
     3721 cgtgcgcagt cggcggagtg cccgacgcgg tcgtcgacgg cgagacgggc ctgctcgtgg
     3781 gccgcgatga cgcgtccgga atgactcgcg cgctgcgctc gctgctcggc tcaccggaga
     3841 cgcgcgcgcg cttcggagcc gcggcgcggc gccacgtcga gcggcgtttc gacgtgagca
     3901 agctcgtcgg cgcgcatcat tccttatata cgcgcgtgct cggcgagcgc gccgccgccg
     3961 cggcgccggt ggggctcgag tgacgccggc ggaccgcgat ctcgaagcat gggcgctgag
     4021 cctcggccgc gctctgaagg cgcgcggctt gcgcgtcgcg acggccgagt cgtgcacggg
     4081 cggctggatc gccaaggcct tgaccgacgt cgcgggcagc tcgcaatggt tcgagggtgg
     4141 cgtcgtcgcc tactcgaacg cggcgaagat ttcgctgctc ggtgtggcga gcggcgtgct
     4201 cgccgcgaac ggcgccgtct ccgaagagac cgtgcgcgcg atggccgaag gcgcgcgcag
     4261 ccgcttcgcc gcggatttcg ccgtcgccgt cagcggcatt gcgggccccg acggcggcag
     4321 cgccgacaag cccgtcggca cggtgcactt cgcctgggcc gcgcccggcg gcacgacggc
     4381 cgcgcgccgc atcttcgcgg gagaccgcga gaccgtgcgg cgtctgaccg tcgcgctcgc
     4441 gctcgagcgg ctcatcgagc tcgcgaccgt agatgagcga cccccttaac gaacggcccg
     4501 accgccaccg cgtgttcttc gcgttgtggc ccgacgacgc gacgcgcgcc gcgatctcgc
     4561 gctcgacgcg cgaggccgtg cgcctctccg gcggccggcc gatcgcgaag gagcggctgc
     4621 acatcaccgt cgcgttcctc ggcgagctca ctgccgcggg cgtcgaggcc gcgcgggcag
     4681 tgccgccgat tccggtcggg ccgttcgagc ttacgctcga cgccatcggc gtgtggccgg
     4741 agtcgaagat tctgtggctc gcgccgacgg ctctgccggc cgcgctcgcg gagctcgaga
     4801 atctgctctg ggatgggctc gtcgagtgtg gctttcgcgg cgcggagcgc gactacctgc
     4861 cgcacgtcac actcgcgcgg cgggcgcggc cggtcgagtc cgccgtcgag cccgtgcgct
     4921 ttccggttcg cgacctcgcg ctcgtcgagt cgtttccgga cggacgcaac gtccactacg
     4981 aggtgctcga gcgctgggac ctctgactca cgaaaatact ggacaaaacc tcagtatcgc
     5041 gattcaatgg cgactcctag tttctggtcg aagtcggtcg attcacgcga ctgccccctt
     5101 tacggaactg tgaaataatt ttgccgcgta tcgtaagccg cgcagccgga aaaccaccca
     5161 cccacgagcg agaatatgga cgacaaccgc aagaaagccc tttccgcagc actcggccag
     5221 atcgagaagc aattcggcaa gggctccgtc atgcgcttgg gcgatgcgat cgcgtcgcgc
     5281 gacatcgagg tcatatcgac gggctcgctc ggcctcgacc tcgcgctcgg cgtcggcggc
     5341 ttgccgcgcg gacgcgtgat cgaaatctat gggccggaaa gctcaggcaa gacgaccctt
     5401 gcgctgcacg cgatcgccga ggcgcagaag aacggcggga ccgccgcttt cgtcgatgcc
     5461 gagcatgcgc tcgatccggt ctatgcgaag aagctcggcg tcgac
//
LOCUS       DASSNN010000521         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_594378, whole genome shotgun sequence.
ACCESSION   DASSNN010000521 DASSNN010000000
VERSION     DASSNN010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594378"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="VFL84_09720"
     CDS             <1..1676
                     /locus_tag="VFL84_09720"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET7608942.1"
                     /translation="DLVERGGRMFGATLGKTEQLEVTLDGARILLADLAEYTVESGAA
                     IRLPVKAGTHSIGAAFIKKNHAPVEDIIQPFDFSLFEPAVDGDPDWTFVPHLASLSVT
                     GPYAVTGVGDTATRRRIFVCRPSSEREEAPCARQIVETLATHAYRHPVDREQLSTLMD
                     FYAVGREQAGFEAGIEMALRRILASPEFVFRFERLGQGVKPGQIYRISDVELASRLSF
                     FLWSSIPDDELLALAVDGKLHEPGTLRKQVRRMLDDPKSRAFVENFAGQWLYLRNLKN
                     KGGSVEHFPSFDDNLRQAFRTETEMLFASIVREDRNLLDLLTADYTFVNERLAHHYGM
                     QGVYGSEFRRVPVENAARRGILGHGSILLVTSLPERTSPVQRGVWVLENIVGAPVPTP
                     PPVVPALEEQAGTKEHPRTLREQMRLHTERPFCAGCHKIMDPVGFAMENFDAIGRWRT
                     DELGVPIDASARLVDGTEIDGMIDLRNALLKYSDRFVQTTTEKLMTYALGRGVEYYDM
                     PTVRAIARGAAKDDYRFSSIILGIVESDAFQMRTAEDPRQATQIVANTD"
     gene            1712..>2076
                     /locus_tag="VFL84_09725"
     CDS             1712..>2076
                     /locus_tag="VFL84_09725"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET7608943.1"
                     /translation="MPRRTFLRGIGATVALPLLDAMVPAMAATPAPRSRYSFLHVPHG
                     ASPGYWRPTGSGKNWELSRILQPLAPFKDRLTVISGTDHRMATSLSPEESAGDHSRTA
                     AVYLTAAHPKRTEGQDIYAG"
BASE COUNT          348 a          717 c          685 g          326 t
ORIGIN      
        1 ccgacctcgt cgagcgcggc ggccgcatgt tcggcgcgac tctcggcaag accgagcagc
       61 tcgaggtcac gctcgacggc gcgcgcatcc tgctcgccga tctcgccgag tacacggtcg
      121 agtccggcgc cgcgatccgg ctgcccgtca aagcgggcac gcattcgatc ggcgcggcgt
      181 tcatcaagaa gaatcacgca ccggtcgagg acatcatcca accgttcgac ttcagcctgt
      241 tcgagccggc agtcgacggc gacccggact ggacgttcgt gccgcacctc gcgagcctgt
      301 cggtgacggg accgtatgcc gtgacgggcg tcggcgatac cgcgacgcgc cggcgcatct
      361 tcgtctgccg gccctcgagc gagcgcgagg aagcgccgtg tgcgcggcag atcgtcgaga
      421 cgctcgcgac gcacgcgtac cgtcatcccg tcgaccgcga gcagctctcg acgctgatgg
      481 atttttatgc cgtgggccgc gagcaagccg ggttcgaagc cggcatcgag atggcgttgc
      541 gccgaattct cgcgtcaccg gagttcgtgt tccgcttcga gcgcctgggc caaggcgtaa
      601 agccgggcca gatctaccgc atctccgacg tcgagctcgc gtcgcgcctc tcgttcttcc
      661 tctggagcag catccccgac gacgagctgc tcgcgctcgc cgtcgacggc aagctccacg
      721 agcccggcac gttgcgcaag caagtgcgcc gcatgctcga cgatccgaag tcgcgcgcgt
      781 tcgtcgagaa cttcgccggt cagtggctct acttgcggaa tctgaagaac aagggcgggt
      841 ccgtcgagca ttttccgagc ttcgacgaca atctgcgcca agctttccgc accgagaccg
      901 agatgctgtt cgcgagcatc gtgcgcgagg accgcaactt gctcgatctc ctgacggccg
      961 attacacgtt cgtcaacgag cgcctcgcgc accattacgg catgcagggc gtctacggca
     1021 gcgagttccg ccgcgtgccg gtcgagaacg cggcgcgccg cggcatcctc ggccacggca
     1081 gcatcctgct cgtgacgtcg ttgcccgagc gcacgtcgcc cgtgcagcgc ggcgtctggg
     1141 tgctcgagaa catcgtcggc gcgccggtgc cgacgccgcc gccggtcgtg cccgcgctcg
     1201 aggagcaggc cggaacgaag gagcatccgc gcacgctgcg cgagcagatg cgcttgcaca
     1261 ccgagcggcc gttctgcgcg ggctgccaca agatcatgga tccggtcggc ttcgcgatgg
     1321 agaacttcga cgcgatcggg cgctggcgca cggacgagct cggcgtgccg atcgacgcga
     1381 gcgcacggct cgtcgacggc accgagatcg acggcatgat cgacctgcgc aacgcgctcc
     1441 tgaagtactc cgaccgattc gtgcagacga cgaccgagaa gctcatgacg tacgcgctcg
     1501 ggcgcggcgt cgagtactac gacatgccga cggtgcgtgc gattgcgcgc ggcgcggcaa
     1561 aggacgacta tcgtttctcg agcatcattt tgggaatcgt cgagagcgac gcgtttcaga
     1621 tgcgcacggc cgaggaccct cgtcaggcga cgcagatcgt cgccaatacg gattgatgga
     1681 gagcatcgcg atgttcatca cgaagcgaca catgccacgc cggactttcc tccgcggaat
     1741 cggtgcgacc gtcgcactgc cgctgctcga cgcgatggtg cccgcgatgg cggccacgcc
     1801 cgcgccacgc agccgctaca gcttcctgca cgtgccgcac ggcgcgtcgc cgggttactg
     1861 gcggccgacg ggctcgggca agaactggga gctctcgcgg atcttgcagc cgctcgcgcc
     1921 gttcaaggac cgcctcaccg tcatcagcgg cacggaccac cgcatggcga cgtcgctgtc
     1981 ccccgaggag agcgccggcg accattcgcg cacggcggcc gtgtatctga cggccgcgca
     2041 tccgaagcgc accgaaggcc aggacatcta cgccgg
//
LOCUS       DASSNN010000522         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_594443, whole genome shotgun sequence.
ACCESSION   DASSNN010000522 DASSNN010000000
VERSION     DASSNN010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594443"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VFL84_09730"
     CDS             complement(<1..1239)
                     /locus_tag="VFL84_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7608944.1"
                     /translation="MRTLRAWLHRLAGSFSSSRREREFADELQSHLQMHIDDQMRAGM
                     TLEEARRSALVALGGVAATEERYRDRGGFPIVAHAAQDLRFAARLFRRSPGFTLMAIV
                     TTMLAVGVNVAIFTAFNAAAFGPLNLPDAGRLVTISTRFDGQYKRNVHGVPSLLSLQE
                     FEAVRDQASSFDGLVGFAPFNQVTLGGAEPRQILVTAASCGYFGALRIRPALGRDLIA
                     SDCVTGAEPVVVLSDALWRSSFSADPSVVGRTVTINRRPFVVVGVAPRGFTGTQLVPE
                     DAFVPLSAQPLLDRNWDQVNGTTASWLFVMGHLRPGASISEARADLDVIAARLSEAAR
                     LASPGVTEHLDVTRSTMSALPEIRTIVLSVGFTLVAGVALVLIIACANIANLLLARAS
                     ARRREIAVRLALGASRARLIK"
     gene            complement(1236..1580)
                     /locus_tag="VFL84_09735"
     CDS             complement(1236..1580)
                     /locus_tag="VFL84_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7608945.1"
                     /translation="MDRDKSDVWQGTLSLMVLKTLETMGPLHGYGIARRIEQTSGNRL
                     IVNYGTLYPALLKLEQEGSIESDWGVSDNNRRAKFYRLTRAGRKQVERSTREWEETTA
                     ILSRFLAPGERP"
     gene            1708..>2179
                     /locus_tag="VFL84_09740"
     CDS             1708..>2179
                     /locus_tag="VFL84_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7608946.1"
                     /translation="MLFATSSLAARIDRSECDTTVAFAQLAKRRYPDVLLQPIGGATA
                     VFAGPGEPFSKLVGLGFDGPLDADVLGRVECEYDRRNAELRVELSTLADNSVGRLLTS
                     RGYILAGHENVLGLELTFDVVARLDKERSPDVEVSPVAPGDARDWIRLVTEAFAH"
BASE COUNT          367 a          720 c          752 g          340 t
ORIGIN      
        1 cttgatcagc cgcgcgcggc tcgcgccaag cgccaggcgg accgcgatct cgcggcgccg
       61 tgccgacgcg cgcgccagca gcaggttcgc aatgttcgcg cacgcgatga tcaacaccag
      121 cgccacgccc gcgacgagcg tgaagccgac gctgagaacg atcgttcgaa tctccggcag
      181 cgccgacatc gtcgatcgcg tgacgtcaag gtgctcggtc actcccggac tggcgagccg
      241 ggccgcctcg gacaggcgcg cggcaatgac gtcgagatcg gcgcgcgctt cgctgatcga
      301 cgcgcccggg cgcagatgcc ccatgacgaa caaccagctg gccgtcgttc cgttgacctg
      361 gtcccagttg cgatcgagga gcggctgcgc ggagagcgga acgaaggcgt cctccggaac
      421 gagctgcgtg ccggtgaagc cgcgcggcgc aacgccgacg acgacgaatg gccgccgatt
      481 gatcgtgacc gtgcggccga ccacggacgg gtcggcggaa aaagacgacc gccacagcgc
      541 gtcgctcagc acgacgaccg gctcggcgcc tgtcacgcag tcggacgcga tgagatcccg
      601 gccgagcgcc gggcgaatgc gaagcgcgcc aaagtagccg cacgatgccg cggtcacgag
      661 aatctgccgc ggctccgcac cgccgagcgt gacctggttg aagggcgcga acccgacgag
      721 gccgtcgaac gagctggcct gatcgcgcac ggcctcgaac tcctggagcg agagcagact
      781 cgggacgccg tgtacgttgc gcttgtattg gccgtcgaat cgcgtcgaga tcgtgacgag
      841 acggccggcg tcgggaagat tgagcggccc gaaagccgcg gcgttgaagg cggtaaagat
      901 ggcgacgttc actccgacgg caagcatggt cgtcacgatc gccatcagcg tgaaacccgg
      961 cgatcgccgg aagagccgcg ccgcaaaccg gagatcctgt gccgcgtgcg cgacaatcgg
     1021 gaagccgcct cgatcgcgat agcgctcttc ggttgccgcg acaccgccca gcgcgacgag
     1081 cgccgaacgg cgggcctcct ccagcgtcat gccggcgcgc atctgatcgt cgatgtgcat
     1141 ctggagatgg ctctggagtt cgtccgcgaa ctcgcgctcg cggcgcgagg agctgaacga
     1201 gccggcgaga cggtgaagcc atgcgcgcag cgttctcatg ggcgttctcc gggcgcgaga
     1261 aatcgcgaca ggatcgccgt cgtctcttcc cactcgcggg tcgagcgctc gacctgttta
     1321 cggccggcgc gagtcaggcg atagaacttt gcgcggcggt tgttgtcgga tacgccccag
     1381 tccgattcga tcgagccttc ctgttcgagc ttcaggagtg cggggtacaa ggttccgtag
     1441 ttcacgatca gccggttgcc gctcgtctgc tcgatgcgcc gcgcgatgcc gtacccgtgc
     1501 agcggtccca tcgtttcgag cgtcttgagc accatcaggc tgagggtgcc ctgccacacg
     1561 tcggatttgt cgcggtccat cgctactcct atggcaaagc catacgaagc gtagcgcgtt
     1621 tcgtatggga aagcaatacg actggcgcca gccgctggtt ccccacgtgg gacctcgacg
     1681 agcagcgtcc ggggtaggct gaagcctatg cttttcgcga cgagctcgct ggcggcgcgg
     1741 atcgaccgct ccgaatgcga caccaccgtg gcgtttgcgc agctcgcgaa gcggcgctat
     1801 ccggacgtcc tcctgcagcc gatcggcggc gcgacggccg ttttcgcggg tccgggagag
     1861 ccgttcagca agctcgttgg actcggcttc gacggaccgc tcgacgccga cgtgctcggg
     1921 cgcgtcgagt gcgagtacga ccggcggaat gcggagctgc gcgtcgagct ttcgacgctg
     1981 gcggacaact cggtcgggcg gctcctcacg tcgcgcggct acatcctcgc gggacatgaa
     2041 aacgtcctcg gcctcgagct gacgttcgat gtcgttgcgc ggctcgacaa ggagcgctct
     2101 cccgacgtcg aggtcagccc ggtcgcgccc ggggacgcgc gcgattggat ccggctcgtc
     2161 accgaggcgt tcgcgcacg
//
LOCUS       DASSNN010000523         5254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1103129, whole genome shotgun sequence.
ACCESSION   DASSNN010000523 DASSNN010000000
VERSION     DASSNN010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103129"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /gene="mobB"
                     /locus_tag="VFL84_09745"
     CDS             <1..360
                     /gene="mobB"
                     /locus_tag="VFL84_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HET7608947.1"
                     /translation="KDSHAHRMAGAREVLISSARRWALMHELRSEPELSLRELLTHLS
                     PVDLVIVEGYKTENHPKLEVHRASVGTPLLYPNDPHIVAIASDARPPNVPLPWADLND
                     VEAIADLVDELARPYPS"
     gene            357..971
                     /locus_tag="VFL84_09750"
     CDS             357..971
                     /locus_tag="VFL84_09750"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HET7608948.1"
                     /translation="MSAQGAVVGLVLAGGRSVRFGGEKAVALLDGRPLLEWAADRLRS
                     VCAQVAINVRAGSEAEAVAQTKALPTLYDEAGDALGPLAGVKVGLIWAQAEGAGLLAV
                     SPCDAPLLPADLYVRLLAHAAGGPAMAETRDGRQPLCAIWPVTALPDVRAALRGGAHP
                     PTWQMLERIGARKVFFDCPDAFANVNTREDLAAVEAEARGTPRS"
     gene            complement(998..1483)
                     /locus_tag="VFL84_09755"
     CDS             complement(998..1483)
                     /locus_tag="VFL84_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7608949.1"
                     /translation="MKSKLIATAIASLALATAVRGAEPVVAASPEAQKKMLESSDPQL
                     AANKKLVYDMWRVFLQGGDINAADKYLAPEYHQHNPNADTGLEGVKAYFRAAGRTATP
                     PKDSIDGLVAITAERDLVMIARLRELKDKEGKAYTTTTFDLFRVANGKIVEHWDIATK
                     P"
     gene            1628..1996
                     /locus_tag="VFL84_09760"
     CDS             1628..1996
                     /locus_tag="VFL84_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7608950.1"
                     /translation="MSIIVRKLRLQRGWTQEDVARFAGLSVRSVQRVERGQPGSLETM
                     KALAAVFETDVATLGNGGQEMSDTTVMPADEKEALEYVARLKGFYTHLFMYVVFAIGF
                     LGMFGLKWGFSSPPVRLKLN"
     gene            complement(2007..2702)
                     /locus_tag="VFL84_09765"
     CDS             complement(2007..2702)
                     /locus_tag="VFL84_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608951.1"
                     /translation="MLKQLRIGRTRCTAAPLVAAAMFLASCADEPVPKLQQIYTGFPE
                     SIDPQCRDGTATIYDECGDQLALFREALARANAENKVLLVEYGAEWCIWCHVFNAYIN
                     GDKGRFRYTYASAKEPEAWSTRTFEEDDDVAGAEELRDFVARHFVVVRIDAQYAPNGA
                     DVLEQSGAAEHFGGGLPFVFTVDTHGHYAATFEGKGLEKRRGSFDWYRGFHRDTMLRV
                     LTSMRNAALASRD"
     gene            complement(2708..3502)
                     /locus_tag="VFL84_09770"
     CDS             complement(2708..3502)
                     /locus_tag="VFL84_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7608952.1"
                     /translation="MLALAAAPSALAQRFITVASTTSTEQSGLFKHLLPLFEQKSGIE
                     VRVVALGTGQALDLARRGDADVVFVHAKAAEEQFLSEGHGVRRFPVMYNDFVIIGPKA
                     DPAGIAHGADAAEALRKIKDARAAFVSRGDRSGTHIAEVNLWTAAGVDIAKEKGPWYR
                     ETGQGMGPALNTASSMNAYLLSDRATWLAFVNRGELAILVEGDKRLLNQYGVMLVSPE
                     KHPSVKRADGQAFIDWLVSPEGQAAIADYKIGGEQLFFPNAGEPEA"
     gene            complement(3532..4242)
                     /locus_tag="VFL84_09775"
     CDS             complement(3532..4242)
                     /locus_tag="VFL84_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET7608953.1"
                     /translation="MLPLILEDVSFVAGGRAIIDGVSCDLAAGPRTVILGPNGSGKSV
                     LMRLCHGLLTPTSGRIVWHGNADEGRPRAQAMVFQRPVMLRRSALANVTYGLELAGVP
                     RAKRERRARDVLDAVGLTHVAGRPARVLSGGEQQKLALARAWALEPEVLFLDEPTANL
                     DPNATREVERIVDEIHASGTKIVMTTHNLGQARRLADEILFMSGGRVADHAPVERFFA
                     EPSTGEAAAFIKGELPWS"
     gene            complement(4248..4952)
                     /locus_tag="VFL84_09780"
     CDS             complement(4248..4952)
                     /locus_tag="VFL84_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7608954.1"
                     /translation="MGDIASAFAEAGRMIVAADGTLWGIVLLSLKVTLTAVAVATAVG
                     LPLGAALAIRRFAGRQVLLVTLNALMGLPPVVVGLVVYLLLSRAGPLGELGLLFTPTA
                     MVIAQAILVTPIIAGLSRQVLEDAWHEYEEQLRSLGATPTAAAATLLWDTRYSLVTVV
                     LAGFGRAAAEVGAVIIVGGNIEGVTRVMTTAISLETSKGDLPLALGLGLILVAVVFLI
                     NAAAQIIKTAAVRRYG"
     gene            complement(5133..5237)
                     /locus_tag="VFL84_09785"
     CDS             complement(5133..5237)
                     /locus_tag="VFL84_09785"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET7608955.1"
                     /translation="MNQYDFSIGSLIVSLLGALILLFIVGLFRRRRHT"
BASE COUNT          813 a         1770 c         1792 g          879 t
ORIGIN      
        1 aaggactcgc acgcgcatcg tatggccggc gcgcgcgagg tgctgatctc gagcgcgcgc
       61 cgctgggcgc tcatgcacga gctgcgcagc gagcccgagc tctcgctgcg cgagctgctg
      121 acgcacctgt cgcccgtcga cctcgtcatc gtcgaaggct acaagacaga gaatcatccg
      181 aagctcgagg tgcatcgcgc gtcggtcggc acgccgctgc tctatccgaa cgatccgcac
      241 attgtcgcga tcgcgagcga cgcgcggccg ccgaacgtac cgttgccctg ggcggatctc
      301 aacgacgtag aggcgatcgc cgatctcgtc gacgagctcg ctcggccgta tccgtcgtga
      361 gcgcgcaggg ggccgtcgtg ggcctcgtgc tcgcgggcgg gcgctcggtg cgtttcggcg
      421 gcgagaaggc cgtcgcgctg ctcgacggcc ggccgctgct cgaatgggcc gcggaccgct
      481 tgcgctcggt gtgcgcgcaa gtcgcgatca acgtgcgcgc gggcagcgag gccgaggccg
      541 tcgcgcaaac taaggcgttg ccgacgctct acgacgaagc gggcgatgcc ttgggcccgc
      601 tcgcgggcgt gaaagtgggt ttgatctggg cgcaagcgga aggcgccggc ctgctcgccg
      661 tgagcccttg cgatgcgccg ctgctaccgg ccgatctcta cgtgcgcctg ctcgctcacg
      721 cggccggcgg ccccgcgatg gccgagacgc gcgacggccg ccagcccctg tgcgcgatct
      781 ggcccgttac ggcgctgccc gacgttcgcg cggcgctgcg cggcggcgcg catccgccga
      841 cgtggcagat gctcgagcgt atcggcgcgc gcaaagtttt cttcgactgc cccgatgcct
      901 tcgcgaacgt caacactcgc gaggatttgg cggccgtcga agcggaagcc cgcggaacgc
      961 cgcgctctta gcgctctcgg cccgggtgcg acggccgtca gggcttcgtc gcgatgtccc
     1021 agtgctccac gatcttgccg ttggcaacgc ggaacaggtc gaacgtcgtc gtcgtgtacg
     1081 ccttgccctc cttgtccttc agctcgcgca gacgcgcaat cattacgaga tcgcgctcgg
     1141 ccgtgatcgc aaccaagccg tcgatcgagt ccttcggcgg tgtggccgtc cgtccggcgg
     1201 cgcggaagta ggccttgacg ccctctaggc cggtatcggc attcggattg tgctggtggt
     1261 actccggcgc gaggtatttg tccgcggcgt tgatgtcgcc gccctgcaag aacacccgcc
     1321 acatgtcgta gaccagcttc ttgttggccg ccagctgcgg gtcgctgctc tcgagcattt
     1381 tcttctgcgc ttcgggcgac gcggctacga cgggctccgc gccgcgcaca gcggtggcga
     1441 gggcgagcga cgcgatggcg gtcgcgatga gcttcgattt catggcggac tctcctggtg
     1501 gttttggtgt cgttattgag gccggagcgc ccgactatac ctgacagtcg cgtgccagtc
     1561 gggccgctga cgtggcgagc ggcgagggtt cgggccaagg tgccgccgtt tcgctaccat
     1621 cgcggctatg tcgatcatcg tccgaaaact gagactgcag cgcggctgga cccaggaaga
     1681 cgtcgcgcgc ttcgccggcc tgagcgtccg cagcgtgcag cgcgtcgagc gcggccaacc
     1741 cggcagtctc gagaccatga aagccctggc cgccgtgttc gaaacggacg tggcaacgct
     1801 aggcaatgga ggacaggaaa tgagcgatac gacggtcatg cccgcggacg agaaggaggc
     1861 gctcgagtac gtcgcgcgcc tgaagggctt ctacacgcat ctgttcatgt acgtggtgtt
     1921 cgcgatcggg tttctcggca tgttcggctt gaagtggggt ttttcgtcgc cgcccgtgcg
     1981 gctcaagctg aactgagacc gatgttctag tctcgcgacg cgagcgcggc gttgcgcatg
     2041 ctcgtcagca cgcgcagcat cgtgtcgcga tgaaagccgc gataccaatc gaacgagccg
     2101 cggcgcttct cgaggccttt accctcgaac gttgccgcgt agtgtccgtg cgtgtcgacg
     2161 gtgaacacga aggggaggcc gccaccgaag tgctcggccg ctccgctttg ctctagaacg
     2221 tctgctccgt tcggcgcgta ctgcgcatcg atgcgaacga cgacgaagtg gcgtgccacg
     2281 aagtcgcgca gctcctccgc tcccgcgacg tcgtcgtcct cctcgaacgt cctcgtcgac
     2341 catgcttcgg gctctttggc cgacgcgtac gtgtagcgga atcggccttt gtcgccgttg
     2401 atgtacgcgt tgaacacgtg gcaccaaatg caccactccg cgccgtattc gacgaggagg
     2461 accttgttct ccgcgttggc ccgcgcgagc gcctcgcgaa acagcgcgag ctgatcgccg
     2521 cactcgtcgt agatcgtcgc cgtgccgtcg cggcattgcg ggtcgatcga ctcggggaag
     2581 ccggtataga tctgttgcag cttcggcacg ggctcgtccg cgcacgatgc gaggaacatc
     2641 gccgcggcga cgagcggcgc cgccgtgcat ctcgttcggc cgatgcgcaa ttgtttgagc
     2701 atgcgactca cgcctccggc tcgcctgcgt tcggaaagaa cagctgctcc ccgccgatct
     2761 tatagtcggc gatcgcggcc tggccctcgg gcgagacgag ccaatcgatg aacgcttgcc
     2821 cgtcggcgcg cttgacgctc ggatgcttct cggggctcac gagcatgacg ccgtactggt
     2881 tcaagagccg cttgtcgcct tcgacgagaa tcgcgagctc gccgcggttc acgaacgcga
     2941 gccacgtcgc ccggtcggag agcaggtacg cgttcatgga cgacgccgtg ttcagcgccg
     3001 ggcccatgcc ttggccggtc tcgcgatacc acgggccttt ctccttcgcg atgtcgacgc
     3061 cggcggccgt ccagagattc acttcggcga tgtgcgtgcc gctgcggtcg ccgcgcgaga
     3121 cgaacgcggc tcgggcgtcc ttgatcttgc gcagcgcctc ggccgcatcg gctccgtgcg
     3181 cgatgccggc cgggtccgcc ttcggcccga tgatgacgaa gtcgttgtac atgacgggaa
     3241 agcggcgcac gccgtggcct tcgctcaaga attgctcttc ggcggccttc gcgtgcacga
     3301 acacgacgtc ggcgtcgccg cggcgcgcga gatcgagcgc ctggcccgtg ccgagcgcga
     3361 cgacgcgtac ctcgattccc gacttctgct cgaacagcgg cagcaggtgc ttgaagaggc
     3421 cggactgctc ggtcgaggtc gtcgaggcga ccgtgatgaa gcgttgcgcg agcgcgctcg
     3481 gcgcagcggc cagcgcgagc atgagaaacg cgatccgcgc gacattcgcc ctcagctcca
     3541 aggcagctct cctttgatga aggccgccgc ttcgcccgtc gacggctccg cgaagaaccg
     3601 ctccacgggc gcgtgatcgg cgacgcgccc gccgctcatg aagagaattt cgtcggcgag
     3661 ccggcgcgcc tggccgagat tgtgcgtcgt catgacgatc ttcgtgccgc tcgcgtggat
     3721 ctcgtcgacg atgcgctcga cctcgcgtgt cgcgttcgga tcgaggttcg cggtcggctc
     3781 gtcgagaaac agaacctccg gctcgagcgc ccacgcgcgc gcgagcgcga gcttctgctg
     3841 ctcgccgccc gacaacacgc gcgcgggacg gccggccacg tgcgtgagcc cgaccgcgtc
     3901 gagcacgtcg cgcgcgcgcc gctcgcgttt cgcgcgcggc acgccggcga gctcgagccc
     3961 gtacgtgacg ttggcaagcg cagagcggcg cagcatgacg ggccgctgaa acaccatcgc
     4021 ctgagcgcgc ggccggcctt cgtcggcgtt gccgtgccac acgatgcgcc ccgacgtcgg
     4081 cgtcaggagc ccatggcaca ggcgcatcag cacgctcttg ccggagccgt tcgggccgag
     4141 gatcacggtg cgcggacccg ccgcgaggtc gcacgacacg ccgtcgatga tcgcgcggcc
     4201 gccggcgacg aacgacacgt cctcgagtat caacggcagc acggcgtcta cccgtaacgg
     4261 cggacggccg ccgtcttgat gatctgcgcc gccgcattga tcaggaacac gacggcgacg
     4321 aggatcagcc caaggccgag cgcgagcggc aggtcgccct tgctcgtctc gagcgagatc
     4381 gcggtcgtca tgacgcgtgt gacgccctcg atgttgccgc ccacgatgat gacggctccg
     4441 acttcggccg cggcccggcc gaagcccgcg agcacgaccg tgacgagcga gtagcgcgtg
     4501 tcccaaagca gcgttgccgc ggcggccgtc ggcgtcgcgc cgagcgagcg cagctgctct
     4561 tcgtactcgt gccacgcatc ttcgagcacc tggcgcgaca gcccggcgat gatcggcgtc
     4621 acgagtatcg cttgcgcgat caccatcgcg gtcggcgtga acagcagacc gagctcgccg
     4681 agcgggcccg ctcgcgacag cagcaagtac acgacgagcc ccacgacgac gggcggaaga
     4741 cccatcaagg cgttcagcgt gaccagcaat acctgccggc cggcaaagcg ccggatcgcg
     4801 agcgcggcgc cgagcggcaa gcccacggcc gtcgcgacgg cgacggcggt cagcgtcact
     4861 ttgaggctca ggaggacgat gccccacagc gtcccgtcgg ctgcgacgat cattcgcccg
     4921 gcctcggcga acgccgatgc tatgtcgccc attcgggtct tggttctgaa gagctgcgtt
     4981 gcatggatta tccaacggcg cagcatccat tgcgcgagcg ccgcagagcg gcacgatacc
     5041 agcgtcgcgc acgcatgcgc acgcgtgacg tggcggcgag ctcgaggtct cgcgtctagg
     5101 cgcgggcatt cgggcggcta tcgccttgaa gctcaggtgt gccggcggcg gcggaatagc
     5161 cccacgatga acagcagtat cagcgcgccg agcagcgaca cgatgagcga gccgatgctg
     5221 aagtcgtatt gattgatcgt gcccgtgccg aaca
//
LOCUS       DASSNN010000524         2934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1474945, whole genome shotgun sequence.
ACCESSION   DASSNN010000524 DASSNN010000000
VERSION     DASSNN010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474945"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VFL84_09790"
     CDS             complement(<1..1193)
                     /locus_tag="VFL84_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET7608956.1"
                     /translation="MRARILLLLLAVCPASAMSQPTFDVMETTVAEIHRAYASGDLTA
                     RQLVQLYLDRIEAYDRNGPRINSIITRNAQALEEADRLDAAYRASGFVGALHGIPVML
                     KDQVDAKGMPTTLGSVLFKDYYPDQDAFVTAKLKEAGAIILAKVTLGEMAAGDTHGSL
                     FGSTRNPYALDRTVGGSSGGPAASIAANFATLAVGQEALASIRRPAAWSSVAGMRPTA
                     GLVSRTGVYAGWPQVAGSLGPIARTVEDVAALLDVLVGYDPDDPITAHGAAHVGGSYT
                     SFLDANGLRGARLGILRESIGIGSEPESEDFAKVDAAFARSVGELRAAGAVLVDPLVI
                     PDLKQLLANRGGRPGDGAEAFANYFARSRHPPFASIEEMRAAPDYDRLTNFAKALFTN
                     DGDTSR"
     gene            1310..>2934
                     /locus_tag="VFL84_09795"
     CDS             1310..>2934
                     /locus_tag="VFL84_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HET7608957.1"
                     /translation="MQAVIVGAGMGGLFGALALRESKQFERIDVYEQTRTPSTAGAGL
                     NIAPNGARLCKWLGVDLDGGDPKGPPGVLDGGRAAILEATCQVQPDGTRTRRPIDHDT
                     AAHDGAGFHHMHRLDLLMCLHKRVAELGPSSGLECPIRVHMGKRLESLEQDAASVTAK
                     FADGSSARGDVLIGADGLNSKVLRLLWPNTPPKRWTQVVVYRGLAPRAAIASLRKADG
                     SPLDYNPIDSYTMDVIKNDYAFAMTYWVRGGELLNIALMYYEPNAAEFEREDWGDWFP
                     IDHAEMQRSMSRAFGDDKRKHDVLALAGAMQNPTKWGLYDRDAFEHWQDGRVCLLGDA
                     AHPMLPTMGQGASQSFEDGAALGSCFKLHGTDVYSALLHYERVRHYRATRFQVASKFL
                     FKHLEPPDSPERRMLLKALNERDFPIFDHEQRAGDDDSWIYAFDARKVGDKLPPKKLG
                     PWDFRARSAALDTRKEIATNLWKPAVSLKGDRKVTRAELARHATFEDCWIVIRGKVYD
                     FTEWKDRHPGGPFVARMYAGKDATAEFGDFHSKL"
BASE COUNT          493 a         1019 c          994 g          428 t
ORIGIN      
        1 cgcgacgtgt cgccgtcgtt cgtgaacagc gccttcgcga aattcgtgag cctgtcgtag
       61 tcgggcgccg ccctcatctc ttcgatcgag gcgaagggcg gatggcggct ccgggcgaag
      121 tagttggcga acgcttccgc gccgtcgccc ggccggccgc cgcggttcgc gagcagctgc
      181 ttcagatccg gaatcacgag cgggtcgacg agcacggctc ccgcggcgcg cagctcgccg
      241 accgaccgcg cgaacgccgc gtcgaccttc gcgaagtctt cggactccgg ctccgagccg
      301 atgccgatcg attcgcgcaa gatgccgagg cgcgcgccgc gcaagccatt cgcgtcgagg
      361 aagctcgtgt agctgccgcc cacgtgcgcc gcgccgtgcg cagtgatcgg gtcgtccggg
      421 tcgtaaccga ccagcacgtc gagcagcgcc gcgacgtcct cgaccgtgcg cgcgatcggc
      481 ccgagagagc cggccacttg cggccaaccc gcgtacacgc ccgtacggct cacgaggccc
      541 gccgtgggcc gcatgcccgc gaccgagctc cacgccgcgg gcctgcgaat cgacgcgagc
      601 gcctcctgcc ccaccgcgag agtcgcgaaa ttggcggcga tgctcgcggc cggcccgccc
      661 gaggagccgc cgacggtgcg gtcgagcgcg taggggttgc gcgtcgagcc gaacaaagag
      721 ccgtgcgtat ccccggcggc catctcgccg agcgtgacct tcgcgaggat gatcgcgccc
      781 gcctctttga gcttcgccgt cacgaacgcg tcttggtccg ggtaatagtc cttgaagagc
      841 accgagccga gcgtcgtcgg catgcccttg gcgtcaacct ggtccttcaa catcaccggg
      901 atcccgtgca gcgcgcccac gaaaccggac gcacggtacg cggcatcgag gcggtccgcc
      961 tcttcgagcg cctgggcgtt ccgcgtaatg atcgaattga tgcgcggccc gttccggtcg
     1021 taggcctcga tgcgatcgag atagagctgg acgagctgcc gcgcggtcaa gtcaccggac
     1081 gcgtaggcgc ggtgtatttc cgcaaccgtc gtctccatga cgtcgaacgt gggctggctc
     1141 atcgcgctcg ccgggcagac ggcgagcagc agcaacagaa ttcttgctct cacggcctcg
     1201 ccacccttcc ccgcacgcgc tgcacgttat atcgactata actcgcggcc cgcttccgcg
     1261 gtgccgttga aggcgcacaa tgccaacgtc gagccgcgag ggggcgaaga tgcaggcagt
     1321 catcgtcggc gccggaatgg gcggcctgtt cggcgcgctg gcactgcgcg agtcgaagca
     1381 gttcgagcgc atcgacgtct acgagcaaac ccgcacgccg agcacggccg gagcgggcct
     1441 caacatcgcg ccgaacggcg cgcgcctctg caaatggctc ggcgtcgact tggacggcgg
     1501 cgacccgaaa gggccgcccg gagttctcga tggcggccgc gccgcgatcc tcgaggcgac
     1561 ctgccaggtg cagccggacg gcacgcgcac gcgccggccg atcgaccacg acacggccgc
     1621 ccacgacggc gcgggcttcc atcacatgca ccggctcgat ctcttgatgt gcctgcacaa
     1681 gcgagtcgcc gagctcggcc cgagctcggg tctggagtgt ccgatccgcg tgcacatggg
     1741 caaacggctc gagtcgctcg agcaagacgc agcgtccgtg accgcgaagt tcgcggacgg
     1801 cagctccgcg cgcggcgatg tactgatcgg cgcggacggg ctcaactcga aggtgctgcg
     1861 gctgctctgg ccgaacactc cgccgaagcg ctggacgcaa gtcgtcgtgt accgcgggct
     1921 cgcgccgcgc gccgcgatcg cgagcttgcg caaagccgac ggcagcccgc tcgactacaa
     1981 cccgatcgat tcgtacacga tggacgtcat caagaacgac tacgcgttcg cgatgacgta
     2041 ctgggtgcgc ggcggcgagc tcctgaacat cgcgctgatg tactacgagc cgaacgccgc
     2101 ggagttcgag cgcgaggact ggggcgactg gttcccgatc gatcacgccg aaatgcagcg
     2161 ctcgatgagc cgcgcgttcg gcgacgacaa gcgcaagcac gacgtgctcg cgctcgccgg
     2221 cgcgatgcag aacccgacga agtggggact ctacgatcgc gacgcgttcg agcactggca
     2281 ggacggccgc gtgtgcctgc tcggcgacgc ggcgcacccg atgctgccga cgatgggcca
     2341 aggcgcgtcg cagtcgttcg aggacggcgc cgcgctcgga agctgcttca agctgcacgg
     2401 cacggacgtc tacagcgcgc tgctccacta cgagcgcgtg cgccattacc gcgcgacgcg
     2461 ttttcaggtc gcgtcgaagt ttctgttcaa gcacctcgag ccgcccgatt cacccgagcg
     2521 gcgcatgctg ctgaaggccc tgaacgagcg cgacttcccg atcttcgacc atgagcagcg
     2581 cgccggcgac gacgactcgt ggatctacgc gttcgacgcg cgcaaggtcg gcgacaagct
     2641 gccgccgaag aagctcgggc cgtgggactt ccgcgcgcgc agcgcggcgc tcgacacgcg
     2701 caaggagatc gcgacgaacc tctggaagcc ggcggtctcg ctgaaaggcg atcgcaaggt
     2761 cacgcgcgcc gagctcgcgc gtcacgcgac gttcgaagac tgctggatcg tcatccgcgg
     2821 caaggtctac gacttcacgg aatggaagga ccgccatccc ggcgggccgt tcgtcgcgcg
     2881 catgtacgcg ggcaaggacg caacggcgga gttcggcgac ttccacagca agct
//
LOCUS       DASSNN010000525         7316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1103410, whole genome shotgun sequence.
ACCESSION   DASSNN010000525 DASSNN010000000
VERSION     DASSNN010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103410"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1854
                     /locus_tag="VFL84_09800"
     CDS             <1..1854
                     /locus_tag="VFL84_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7608958.1"
                     /translation="ARPTAFPQHADRKSYWAYLFARLKPGVSLDEATAAINAPYRAIV
                     NDVDAPLLTGVSEQQLAAFRARTIVLAPGDRGQSRIDDNASTPLNILIVSTGLVLLIA
                     CVNVANLLLARGSTRIGEIAVRASLGASRRRLLSLLLVETLLLAAAAALVSLPLTVGA
                     LRGINAMLPPSNRVFDLTLDGAVVAATMGLAAVSTLVFGLIPAMKLIRVDVNPAQQTQ
                     GARQTGGKAAARFRATLTTAQIALSMALLVLAGWFAQSLANVARVDLGFRAESLAVFT
                     IAPDRNGYSQAQTAALFARLEEEFAQIPGVTAAGAAGIALLDNSNWHNNVSVEGFEAT
                     PETDTDVSRNVVSTRFFETLEMPLLRGSGFESASSDGPGIAIVNERFVEKFGLGDGAV
                     GKRIAFGTSQDLNIEIVGVVRDAKYSEVKADSPPQVFVPRTQAPFMGEMNFYLRSNLR
                     LPELRSAVMGLLARHDANLPLINSLTVPQQARENVFLDRFMGTLATALAAMAVVLAAI
                     GIYGVLSYGVLQRLREIGLRIALGAAPRAVRGMVLKQIAWMAAVGVAIGVGLALLLGE
                     VGRALLFGLRPTDPIVPAAAVLTLLAVVAAAAYLPARRAAQVDPVTALRGD"
     gene            1893..2690
                     /locus_tag="VFL84_09805"
     CDS             1893..2690
                     /locus_tag="VFL84_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7608959.1"
                     /translation="MNATAPLTQSAPLIQLDDLKKVFYTDEVETHALSGVHFEITKGD
                     YLSIAGPSGCGKSTLLSILGLLDTPSGGRYVLNGTPVENLDASQRAQIRNKEIGFIFQ
                     AFNLIGDLTVFENVELPLTYRAGMKAAERKKRVLEALERVRMAHRLNHYPAQLSGGQQ
                     QRVAVARALVGSPSILLADEPTGNLDSKNGEQVMALLSDLHADGATICIVTHDPRYAD
                     YADRKIHMFDGRVVDEETLNRLREEEDRRIAEQARRGRPASADLKED"
     gene            2785..5265
                     /locus_tag="VFL84_09810"
     CDS             2785..5265
                     /locus_tag="VFL84_09810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7608960.1"
                     /translation="MWDVAVRDFRHAARSLARSASFTLVAVATLAIGIGATTAVFSVV
                     DGVLLKPLPYPEPDELVAVWHDAPGAPGLTAVAGGLLMSASMVVTYQDESQAFDKIGG
                     WVTTLENVTGLAEPEQVTAVQVTSETLEAFGIPPLMGRWIGLEDEATNGPLVAMLSYD
                     YWQARFGGDLNVVGRLIEVRGFSSEIVGVMPRGFKFGDTAPELILPANINRSNLMPPP
                     FFFNGIGRLRDGVTLEQASAEIERMLPIWIERFPFPGGASGRQVYLDTWRVGPALRPF
                     KDDVVGGVRDVLWIVMAMIGIVLVIASANVTNLLLVRGEGRAVELDVRAALGASSSRI
                     ARSLLLESGLLALLGGLAGLAVAYGALRALLKMAPPQLPRLDTIGIDARSLAFALLVT
                     FGAAVLFSVAPMLRALRARLSTSLRGARGASRGRAQHRAQNAVVVGQVALALVLLVSS
                     GLMIRTFEALRAVEPGFAEPESLQTFRINVPLQLVPDPHAVWRLERAIEDAVEAIPGV
                     DSAAFTSALPMESGRLSWNTIDVEGVDASQTTALRIFNNISPGYLGTMGMTIVAGRDL
                     DAADVDELRPVALVSEALARELWGTPDAALGHRIRSVSAWREVIGVVADVRMNGLDEA
                     PPSTVYWPTLMADYLLQPLVVWNGVAFAVRSSQAGSPGLTRQIEQAVWSVNPSLPVAS
                     MRTMQDIYDGSLGRTSFTLVLLVAAAGAALVLGIVGLYGVLSYAVSTRRREIAIRFAL
                     GARTRDVQARIVRQGVVLAGIGAAIGVVAAAGVTRLMTSLLYEVEAVDPLTYAAVAAG
                     LIAVAALASYLPARRAATVDPAESLAAE"
     gene            complement(5362..5772)
                     /locus_tag="VFL84_09815"
     CDS             complement(5362..5772)
                     /locus_tag="VFL84_09815"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET7608961.1"
                     /translation="MKYVLDTNAVSALMKGEPAAVERLLSIHREDVAIPQPVIAEIAY
                     GIERLPRSKRRERLQERFDLICAELARAAWTDAVSERFGHVKATLERRGERIEDFDAA
                     IAAHALAEDATLVTANLADMKRVPGLRIDDWAAP"
     gene            complement(5769..6017)
                     /locus_tag="VFL84_09820"
     CDS             complement(5769..6017)
                     /locus_tag="VFL84_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HET7608962.1"
                     /translation="MATSVHLPKELLDAVDRKARALRISRNQLIVRALEREISAGSEW
                     SPGFSERLAERDPDTARDVDELLAGVVAARRSKRATPL"
     gene            6159..6413
                     /locus_tag="VFL84_09825"
     CDS             6159..6413
                     /locus_tag="VFL84_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608963.1"
                     /translation="MDAWTGNVRYAVRVLLRTPGFTLIEELEPLRAVYGVAPLAQRIG
                     NEYSDDRLRTSALALLAGTAPLAALLPAWRASRVEPMKVL"
     gene            6427..>7316
                     /locus_tag="VFL84_09830"
     CDS             6427..>7316
                     /locus_tag="VFL84_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7608964.1"
                     /translation="MSFRQDVYYALRALAGRRLYALASIAVLAIAIGANTTVFSVFNG
                     LFLRPLPYPDGDRLVMVYDSYPKIDLPNAGTAIPDYLDRRDQAQSLEGLGVVARAPRT
                     LSSEGPPQRLLIARASASLFDVLRVGPALGRAFTADEETLGNDRVAVLSYALWNTRFG
                     GRRDILDSSIRLDGESYRVVGVMPRDFGFPDRDTDVYLPFAFTEQQRSDAARGNQFSI
                     SVGRLRAGATIEGLNAELEAIVARNVAAGRLEADTVTVAGFTGRAQPLRDVQVGSLRQ
                     VLAILQGIVILVLLIACANLA"
BASE COUNT         1096 a         2429 c         2497 g         1294 t
ORIGIN      
        1 gcgcgcccga ctgcgttccc gcaacacgcg gaccgcaaga gctactgggc ctatctattc
       61 gcgcgcttga agccgggcgt ctcgctcgat gaagcgacgg cggcgatcaa cgcgccgtac
      121 cgcgccatcg tcaacgacgt cgatgcgccg ctgctgacgg gcgtcagcga gcaacaactg
      181 gccgcgtttc gcgccaggac catcgtgctc gcgccgggcg accggggtca aagcaggatc
      241 gacgacaacg cgagcacccc gctcaacatt ctgatcgtgt cgacaggact ggtgctgctc
      301 atcgcatgcg tgaacgtcgc gaacctgttg ctcgcacgcg gctcgacgcg catcggcgag
      361 atcgccgtgc gtgcctcgct cggtgcatcg cgccgccggt tgctctcgct gttgctcgtc
      421 gagacgctgc tgctcgcggc cgcggcggcg ctcgtgagcc tgccgctcac agtgggcgcg
      481 ttgcgcggca tcaacgcgat gctgcccccg tccaaccggg ttttcgatct gacgctcgac
      541 ggcgcggtcg ttgcggcgac gatggggctc gccgccgttt cgacgctcgt gttcggcttg
      601 atacccgcca tgaagctcat ccgagttgac gtgaacccgg cgcagcagac gcagggcgcg
      661 cggcagaccg gcggcaaggc cgccgcgcgt tttcgcgcga cgctcacgac tgcgcagatc
      721 gcgctgtcga tggcgttgct cgtgctcgcc ggatggttcg cgcagagtct cgccaacgtc
      781 gcgcgcgtcg atctcggctt ccgcgccgag tcgctcgcgg tcttcacgat cgcaccggat
      841 cgcaacggtt actcccaggc ccaaacggct gcgctgttcg cgcgcctcga ggaggaattc
      901 gcgcagatcc cgggcgtcac ggccgccggt gccgcgggca tcgcgttgct cgacaacagc
      961 aactggcaca acaacgtcag cgtcgaaggc ttcgaagcga cgcccgagac cgacaccgac
     1021 gtttcccgca atgtcgtgag cacgcggttc ttcgagacac tcgagatgcc gttgctgcgg
     1081 ggctcgggat tcgagagcgc ttcgagcgac ggtccgggca tcgccatagt taacgagcgg
     1141 ttcgtcgaga aattcgggtt gggcgacgga gccgtcggca agcgcatcgc cttcggtacg
     1201 agccaagacc tcaatatcga gatcgtaggc gtcgtgcgcg acgccaagta cagcgaggtc
     1261 aaagcggact cgccgccgca ggtcttcgtg ccgcgcacgc aggccccgtt catgggcgag
     1321 atgaatttct atctgcgcag caacttgagg ctcccggagc tgcgctcggc ggtgatgggc
     1381 ttgctcgcgc gtcacgacgc gaatctgccg ctcatcaatt ccctcacggt tccgcagcag
     1441 gcgcgcgaga acgtattcct cgaccgcttc atgggcacgc tggccacggc gctggccgcc
     1501 atggccgtgg tgctggccgc catcggtatc tacggcgtcc tgtcgtacgg cgtgttgcag
     1561 cggttgcgcg agatcggcct gcgcatcgcc ctcggcgccg cgccccgcgc cgttcgcgga
     1621 atggtgctca agcaaatcgc ttggatggcg gctgtcggcg tcgcgatcgg cgtcgggctc
     1681 gcattgctgc tcggcgaggt agggcgcgcg ctgctgttcg ggctcaggcc gacggacccc
     1741 atcgttcccg ccgcagccgt gctaactttg cttgccgtcg tagctgccgc cgcgtacctg
     1801 ccggcgcggc gcgcggcgca ggtcgatccg gttactgcat tgcgcggcga ctgagccgct
     1861 ccgaaacttg gattcccttc gaggacacaa cgatgaacgc aaccgctccc ctgacccaaa
     1921 gcgccccctt gattcagctc gacgacttga agaaggtgtt ctacaccgac gaggtcgaga
     1981 cgcacgcgct ctcgggcgtg cacttcgaga tcacgaaagg cgactacttg tcgatcgcgg
     2041 gcccgtcggg ctgcggcaag tcgacgctgc tgtcgatctt aggtctactc gacacgccga
     2101 gcggcgggcg ctacgtgctg aacggcacgc ccgtcgagaa cctcgacgcg tcgcagcgcg
     2161 cgcagatccg caacaaggag atcggcttca tcttccaggc gttcaacctg atcggcgatc
     2221 tcacggtgtt cgagaacgtc gagctgccgc tgacgtaccg cgccggcatg aaggccgcgg
     2281 agcgcaagaa gcgcgtgctc gaggcgctcg agcgcgtgcg catggcgcac cggttgaacc
     2341 actatccggc gcagctctcg ggcggtcagc aacagcgcgt cgccgtcgcg cgcgcgctcg
     2401 tgggcagccc gtcgatcctg ctcgccgacg agccgacggg gaacctggac tcgaagaacg
     2461 gcgagcaagt catggcgctg ctctcggatc tgcacgccga cggagcgacg atctgcatcg
     2521 tcacgcacga cccgcgctac gccgactatg cggatcgcaa gatccacatg ttcgacggcc
     2581 gcgtcgtcga cgaggagacg ctgaatcgcc tgcgcgagga ggaagaccgc cgcatcgccg
     2641 agcaggcgcg ccgcggccgg cccgcgtccg ccgatctcaa agaagattga tgcgtcgcta
     2701 acgcttcgta gggtcgccgc gttcgtgccg gcgcggcgtg cgcgctccct gatccggcgg
     2761 actcacgcag cggagtaacc gatcatgtgg gacgtggcgg tccgagactt ccggcatgcg
     2821 gcgcgaagcc tcgcgcgcag cgcgtcgttt acgctcgttg cggtcgcgac actcgccatc
     2881 ggcattggcg ccaccacggc cgtgttcagc gtggtcgacg gcgtgctgct gaagccgctg
     2941 ccgtaccccg agcccgacga gctcgtcgcc gtctggcacg acgcgcccgg cgcgccgggg
     3001 ctgactgccg tggccggcgg cttgctgatg tcggcgtcga tggtcgtcac gtatcaagac
     3061 gagagccagg cgttcgacaa gatcggcggc tgggttacga ccctggagaa cgtcaccggc
     3121 ttggcggaac ccgagcaggt cacggccgtg caggtcacga gcgagacgct cgaggcattc
     3181 ggtattccgc cgttgatggg ccgctggata gggttggagg acgaagcgac caacggaccg
     3241 ctcgtcgcga tgctctccta cgactattgg caggcgcgct tcggcggcga tctcaatgtc
     3301 gtcggccggc tcatcgaggt ccgaggtttc agctcggaga tcgtcggcgt gatgccgcgc
     3361 ggcttcaagt tcggggacac ggcacccgag ctgatcttgc ccgccaacat caatcgctcg
     3421 aacctgatgc cgcctccatt cttcttcaac ggcattgggc gtctgcgcga cggcgtgaca
     3481 ctcgagcaag cgagcgccga aatcgagcgg atgctgccga tctggatcga gcggtttccg
     3541 ttcccaggcg gcgccagcgg ccggcaggtt tatctcgaca cgtggcgcgt ggggccggcg
     3601 ctgcggccgt tcaaggacga cgtcgtcggc ggggtgcgcg acgtgttgtg gatcgtgatg
     3661 gcaatgatcg gcatcgtgct cgtgatcgcg agcgccaacg tcacgaacct acttctcgtg
     3721 cgcggcgagg gccgcgccgt cgagctcgac gtgcgcgcgg cactcggcgc gagctcgagt
     3781 cgcatcgcgc gctcgttgct gctcgagagc gggttgctcg cgttgctcgg cggcttggcc
     3841 gggctcgcgg tcgcctacgg cgcgttgcga gcgctgctta agatggcgcc accgcaattg
     3901 ccgcgcctcg acacgatcgg catcgacgct cgctcgctcg cctttgcgtt gctcgtgaca
     3961 ttcggggcag cggtcctctt cagcgtcgcc ccgatgttgc gcgccttgcg ggcgcggctc
     4021 tcgacgagct tacgcggagc gcgcggtgca agccgtgggc gcgcgcagca tcgcgcacag
     4081 aacgctgttg tcgtggggca ggtcgcgctt gcgctcgtgc tgctcgtgag ctcgggtctc
     4141 atgattcgca cgttcgaggc cctgcgcgcg gtcgagcccg ggttcgctga gcctgagtcc
     4201 ctgcagacgt tccgcatcaa cgtgccgctg cagctcgtgc ccgatccgca cgccgtttgg
     4261 agactcgagc gagcgatcga ggatgcggtg gaagccattc cgggcgtcga ctcagccgcg
     4321 ttcacgtccg cattgccgat ggaaagcggc cgcttgagct ggaacaccat cgacgtcgaa
     4381 ggcgtcgatg cgagccagac gacggcgcta cgcatcttca ataacatctc tccgggctat
     4441 ctcggcacga tgggcatgac gatcgtggcg gggcgcgacc tcgacgcggc cgacgtcgac
     4501 gagcttcgcc ccgtggcgct cgtttcggaa gcgctcgctc gcgagctctg ggggacgccc
     4561 gacgcggcgt tgggccatcg aatccggtcc gtcagcgcgt ggcgcgaggt catcggcgtc
     4621 gtcgccgacg tgcgtatgaa cgggctcgac gaagcgccgc cctccacggt gtattggccg
     4681 accctgatgg ccgactatct actacagccg ctggtcgtct ggaacggcgt cgcgttcgcg
     4741 gttcggagct cgcaagcggg ctcgcctggg ctgacacgcc agatcgagca ggccgtctgg
     4801 tccgtcaacc cgagcttgcc tgtcgcgtcg atgcgcacga tgcaggacat ctatgacggc
     4861 tctctcggcc gcacgtcgtt cacgctcgtg ctgctcgtcg ctgcagccgg cgctgcgctc
     4921 gtgctcggca tcgtcgggct ctacggcgtg ctctcgtacg cggtgtcgac gcgccgccgc
     4981 gagatcgcga tccggtttgc gctcggcgcg cgcacgcgcg acgtgcaagc ccgcatcgtt
     5041 cggcaagggg tggtgctcgc gggtatcgga gccgcgatcg gcgtcgtcgc ggccgccggc
     5101 gtcacgcggc tcatgacgtc gttgctctac gaagtcgagg ccgtcgatcc gctgacgtac
     5161 gcggccgtgg cggccgggct catcgcggtc gccgcgctcg cgagctactt gccggcgcgg
     5221 cgcgccgcga ccgtcgatcc ggcggaatcg ctggccgcgg agtagcgcga tatggctggg
     5281 cctgaaggga tcattgctac gccacggcga ggacgtgcat cttcagtcac gtacgttcaa
     5341 gcgcccgaac gatggggctg cttatggggc ggcccaatcg tcgattctga gaccgggcac
     5401 acgtttcatg tcggcgagat tggcggtgac gagcgtggcg tcttccgcga gtgcgtgcgc
     5461 agcgatggcg gcgtcgaaat cctcgattcg ctcgcccctg cgctcgagcg tggccttgac
     5521 gtggccgaag cgttcgctga cggcgtccgt ccaagcggct cgcgcgagct cggcgcaaat
     5581 gaggtcgaac cgctcttgca gccgctcgcg acgcttggaa cggggcaagc gctcgatgcc
     5641 gtaggcaatc tcggcgatca cgggttgcgg tatcgcaacg tcctcgcggt gaatcgacag
     5701 cagccgttcc acggcggccg gttcgccctt catgagcgcg gaaacggcgt tcgtatccag
     5761 cacgtatttc acaacggcgt tgcgcgtttc gagcgccgcg cggccacgac gccggcgagc
     5821 agctcatcca cgtcgcgcgc cgtatcgggg tcccgttcgg ccaggcgctc ggagaagccc
     5881 ggcgaccatt ccgagccggc gctgatttcc cgctcgagcg cccggacgat cagctggttg
     5941 cggctgatgc gcagcgcccg agctttgcga tcgacggcgt ccagcagctc tttcggtagg
     6001 tgtacgctcg tggccatgac tatattcgcg aatatagtca cgcacgctga cgcttgcaag
     6061 ttgccatcga cacgcacggc cagtcgttgc ctgcgcgtaa ccgttccgcg gccgagcgta
     6121 tctaggctcc atatgcgcac cgaagcatag gagcacccat ggatgcttgg accggcaacg
     6181 ttcgttacgc ggtccgcgtg ctcctcagga cgccggggtt cacgctgatc gaagagctcg
     6241 agccgctgcg cgccgtctac ggcgtcgcgc cgctggcgca gcgcatcggc aacgagtact
     6301 ccgacgatcg gctgcgcacg tcggcgctcg cgttgctcgc gggcacggcg ccgttggccg
     6361 cgttgctgcc ggcctggcgc gcctcgcgcg tcgagccgat gaaagtattg tgagaggagt
     6421 gatccgatga gtttcagaca ggacgtctat tacgcgctgc gcgcgctcgc cggccggcgg
     6481 ctttacgcgc tcgcgtcgat cgccgtgctc gcgatagcga tcggcgcgaa cacgaccgtg
     6541 ttcagcgtgt tcaacggcct cttcttgcgc ccgttgccct atcccgacgg cgaccggctc
     6601 gtgatggtct acgactcgta tccgaagatc gacttgccga acgccggcac ggcgattccc
     6661 gactatctcg atcgacggga tcaagcacag tcgctcgagg gtttgggcgt agtcgcacgg
     6721 gcgccgcgca cgctgtcgag cgaaggaccg ccgcagcgtc tgctcatcgc gcgtgcgtcg
     6781 gcatcgttgt tcgacgtgct gcgggtaggc ccggcgctcg gccgcgcgtt taccgccgac
     6841 gaggagacct tgggcaacga ccgcgtcgcg gtcctgagct acgcgctctg gaacacgcgg
     6901 ttcggcggca gacgcgacat tttggatagc agcattcgcc tcgacggtga gtcgtatcgc
     6961 gtcgtcggcg tcatgccgcg cgactttggt tttccggacc gcgacaccga cgtctacctg
     7021 ccgttcgcgt tcacggagca gcagcgcagc gacgctgcgc gcggcaacca gttctcgatc
     7081 agcgtcggcc ggttgcgtgc gggcgccacg atcgaagggc tgaatgcgga gctcgaggca
     7141 atcgtcgcgc gcaacgtcgc ggccggccgc ctggaagcgg atacggtgac ggtcgccggc
     7201 ttcacgggcc gagcgcagcc gttgcgcgac gtgcaggtcg gttcgttgcg acaggtgctc
     7261 gcgatcttgc aggggatcgt gatcttggtg ctgctgatcg cgtgcgccaa cctcgc
//
LOCUS       DASSNN010000526         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1838525, whole genome shotgun sequence.
ACCESSION   DASSNN010000526 DASSNN010000000
VERSION     DASSNN010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1838525"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VFL84_09835"
     CDS             <1..1056
                     /locus_tag="VFL84_09835"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HET7608965.1"
                     /translation="IDGRYTVFAVKPTADARGASKEAAAATAAYRVLLGAYPDQQTTV
                     LDPAYAASLAAIPDGPAKTKGIAIGAEVAAAWLALRAGDGREAVVPYVFQVPAPGVYQ
                     RTPPAFPNALAPWLAVMRPFALTTPSQFRAPGPPDLTSDIYAQDVAFTQALGAANSTE
                     RSAEETEIARFYTESPTTFFSHNLREFAAAKNLGVTENARLFAQVFATTADSMIGCWD
                     SKYYYNFWRPVTAITTDLDDGNPATVRDPAWTPLAPTPPHPEYPAGHGCVSTGYAEAL
                     KHFFGSPHLDVTLTSTVAGTVPHVFDNTNKMIDEIILARVAGGMHFQTSIEDGSTLGK
                     KVARWVARNHFKPVGKR"
     gene            1181..1873
                     /locus_tag="VFL84_09840"
     CDS             1181..1873
                     /locus_tag="VFL84_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET7608966.1"
                     /translation="MKRIALVGDYSESVVAHRAIPQALKSAASELRADVTWEWLASRD
                     LPPTGAARLEPYAGFWCVPGSPYENMQGVLDAIRFARVQKRPFLGTCGGFQHALLEYA
                     QAVWDIEALHAETNPEAEDPVIAPLACSLVEVTAALRFERGSRLRAIYGRDSADEEYH
                     CNYGLNPRYAERLRSGPLTIAARDAEGGVRAVELRDHPFFIGTLFQPERSALRGQTPP
                     LVKAFVEAVARG"
     gene            complement(1892..>2228)
                     /locus_tag="VFL84_09845"
     CDS             complement(1892..>2228)
                     /locus_tag="VFL84_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7608967.1"
                     /translation="AAAPFLPEMPPRKPDEPGQFAFADRARVESILRSAGWTGIDVQP
                     LDVVCTLPERDLTPYITRLGPLGRVLQQVGEQQRARIVETVRAAFDPYVQGTQVRFTA
                     ACWTVAAHA"
BASE COUNT          346 a          770 c          767 g          345 t
ORIGIN      
        1 atcgacggcc gctacaccgt gttcgcggtc aagccgacgg cggacgcgcg cggcgcttcg
       61 aaggaggcgg cggccgcaac ggccgcctat cgcgtcctgc tcggtgccta ccccgatcag
      121 cagacgaccg tgctcgaccc ggcctacgcg gcgtcgctcg ccgcgatccc cgacgggccc
      181 gcgaagacga agggcatcgc gatcggcgcc gaagtcgcgg ccgcctggct cgcgctgcgc
      241 gccggcgacg gccgcgaggc cgtcgtgccg tacgtcttcc aggtgccggc accgggcgtc
      301 tatcaacgca cgccgcccgc gttcccgaat gcgctcgcgc cgtggctcgc cgtgatgcgg
      361 ccgttcgcgc tgacgacgcc gtcgcagttt cgcgcgcccg ggccgccgga tctgacgagc
      421 gacatctacg cgcaggacgt cgcgttcacg caagcgctcg gggccgcgaa tagcacggaa
      481 cgttcggccg aagaaacgga gatcgcgcgg ttctacaccg agagtccgac gacgttcttc
      541 tcgcacaact tgcgcgaatt cgcggcggcg aagaacttag gtgtaaccga gaatgcgcgc
      601 ctgttcgcgc aggtcttcgc gacgacggcc gacagcatga tcggctgctg ggactcgaag
      661 tactactaca acttctggcg tcccgtcacc gcgatcacga ccgatctcga cgacggcaac
      721 ccggcgacgg tacgagatcc cgcttggacg ccgctcgcgc cgacgccgcc gcaccccgag
      781 tacccggccg ggcacggctg tgtatccacg ggctatgccg aggcgctgaa gcacttcttc
      841 ggctcgccgc acctggacgt cacgctcacg agcaccgttg cgggcacggt gccgcacgtg
      901 ttcgacaaca cgaacaagat gatcgacgag atcatcctcg cgcgcgtggc cggcggcatg
      961 cattttcaga cgtcgatcga ggatggctcg acgctcggca agaaagtcgc gcgctgggtc
     1021 gcgaggaatc acttcaagcc cgtcggcaag cgctagcgaa cgacgcgtcc ccccgcgttg
     1081 ggagtcggcc ggccgaagcg ccggccgact ctatttggcg cgtgcgtttg gctttgcgtg
     1141 cgccctaaag tatcgtcgcg ctcgtgagcg agcggcggcg atgaaacgca ttgcactcgt
     1201 cggcgactac agcgagagcg tcgtcgcgca tcgcgcgatt ccgcaggcgc tgaagtcggc
     1261 tgcgagcgag ttgcgcgccg acgtgacgtg ggaatggttg gcgtcccgag acttgccgcc
     1321 gaccggagcc gcgcggctcg agccgtatgc gggcttctgg tgcgtgcccg gaagcccgta
     1381 cgaaaacatg caaggcgtgc tcgacgcgat tcgcttcgcg cgggtgcaga aacggccttt
     1441 cttgggaact tgcggcggat tccagcacgc gctgctcgag tatgcgcagg ccgtgtggga
     1501 catcgaggcg ctgcacgccg aaacgaatcc cgaggccgag gatcccgtca tcgcgccttt
     1561 ggcctgctcg ctggtcgagg tcaccgccgc gctgcgcttc gagcgcggct cgcggctgcg
     1621 agcgatctat ggccgcgatt cggccgacga ggaatatcac tgcaactacg ggctcaatcc
     1681 gcgctacgcg gagcggctgc gaagcggccc gcttacgatc gcggcgcgcg acgccgaggg
     1741 cggcgtgcgc gccgtggagc tccgcgatca tccattcttc atcggcacgc tgtttcagcc
     1801 cgagcgctcc gcgctgcgcg gacagacgcc gccgctcgtc aaggcgttcg tcgaggccgt
     1861 cgcgcgcggc tagccgccgg ccacatccgc gtcatgcgtg cgcggcgacc gtccagcatg
     1921 ccgccgtgaa gcgcacctgc gtgccctgca cgtaagggtc gaacgccgcg cgcaccgttt
     1981 cgacgattcg cgcgcgctgt tgctcgccga cctgttgcag cacgcggccg agcggtccga
     2041 gcctcgtgat gtagggcgtt aggtcgcgct ccggcagcgt gcagacgacg tcgagcggct
     2101 ggacgtcgat gcccgtccag cctgcgctgc gcagaatcga ctcgacgcgg gcgcgatccg
     2161 cgaaggcgaa ttggcccggc tcgtccggct tgcgcggcgg catctcggga aggaacggcg
     2221 ccgcggcg
//
LOCUS       DASSNN010000527         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1222114, whole genome shotgun sequence.
ACCESSION   DASSNN010000527 DASSNN010000000
VERSION     DASSNN010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222114"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..920)
                     /locus_tag="VFL84_09850"
     CDS             complement(174..920)
                     /locus_tag="VFL84_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7608968.1"
                     /translation="MTVIYVLWLREIKRYLRSRAQIIGSLAQPLLYLLVLGFGFGAVF
                     RQAGQGSYLQFVAPGVIAMGVLFTSIFSGIGLLWDRQFGFLKETLVAPVPRLTIMAGR
                     TLGGATIAIIQGTLVLVVCMIVGFRPENWAAVPVALMFMALIAIVFAALGTAIGSTLK
                     DMQGFQLIMNFLIMPIFFLSGALFPLNNLPAALTVATRLDPLAYGVDGLRGAFIGISA
                     MGAILDVSVLAVLSGLFLVFGAWAFSRIQV"
     gene            complement(917..1726)
                     /locus_tag="VFL84_09855"
     CDS             complement(917..1726)
                     /locus_tag="VFL84_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7608969.1"
                     /translation="MEAQALNKVFGDPKSDKAVRAVQDVTFSVKPGEIFAFLGPNGAG
                     KTTTIQMLTTLLKPTSGSVRIDGLDPTVSPLEVRKRFGIVFQDPSLDGELTAYENMEL
                     HGVLYHVPRKLRTERIELLLMLFELWDRRTSLVKTFSGGMRRRLEIARGLLHTPKILF
                     LDEPTLGLDPQSRNQLWTHVKNLNANEGVTVFLTTHYMDEAEKAAQRVAIIDHGCIVA
                     QGSADELKAQTGTDSLEGAFLKLTGTTIRDERGTAQDAMRQMAKMWQGGRR"
     gene            complement(1785..>2272)
                     /locus_tag="VFL84_09860"
     CDS             complement(1785..>2272)
                     /locus_tag="VFL84_09860"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET7608970.1"
                     /translation="VEQWGGAFVRHAGSGDERVPMALVEPAQRIYSDLAATQRNPALL
                     HGDLHHYNVLSDRERGWCAIDVKGVVGELEYELGAALRNPIERPDLFATLDVVERRLE
                     RFGLALGLDVGRARGWCFAQAVLSAIWSAEHENDAEREAALTLARALFDSSALKADYL
                     D"
BASE COUNT          399 a          779 c          741 g          353 t
ORIGIN      
        1 gcgcgacatc agctcggact tcgacgccgc gacgttgctg accgagctcg tgccgcacct
       61 gccgcgcgac gagagcgtgc tgcaagccta ccgcgagaca ctcgacacga tcggcagcga
      121 tttcgaccgc ggccgcgcgg ccgccgcgct cgaaggcgcg cggcgctagc ccgctaaacc
      181 tggattctcg agaacgccca tgcgccgaac acgaggaaca gccccgacag cacggcgagc
      241 accgagacgt cgaggatcgc gcccatcgcg ctgatgccga tgaacgcgcc gcgcaagccg
      301 tcgacgccgt acgcgagcgg gtcgagccgc gtcgcaaccg tcagcgcggc cggcaagttg
      361 ttcagcggga acagcgcgcc cgacaagaag aagatcggca tgatcaggaa gttcatgatc
      421 agctgaaagc cctgcatgtc cttcagcgtc gagccgatcg cggtgccgag cgccgcaaaa
      481 acgatcgcga tcagagccat gaacatgagc gcgacgggca cggcggccca gttctcgggg
      541 cggaaaccga cgatcatgca gacgacgagc acgagcgtgc cttgaatgat cgcgatcgtg
      601 gcgccgccga gcgtgcggcc ggccatgatc gtgagccgcg gcacgggcgc tacgagcgtt
      661 tccttcaaga agccgaactg ccggtcccag agcaggccta tgcctgagaa gatcgacgtg
      721 aacagcacgc ccatcgcgat gacgccgggc gcgacgaact gcaggtagct gccctgcccc
      781 gcctgcctga acacggcgcc gaagccgaag ccgagcacga gcagatagag cagcggctgc
      841 gcgagcgagc cgatgatttg cgcgcgcgag cgcaggtagc gcttgatctc gcgcagccac
      901 aacacgtaga tcacggtcat cgccgtcctc cctgccacat cttcgccatc tgccgcatgg
      961 cgtcctgggc cgtgccgcgc tcgtcgcgaa tcgtcgtgcc ggtcagcttc aagaacgcgc
     1021 cttcgagcga gtcggtgccc gtctgcgcct tgagctcatc ggcgctgccc tgcgccacga
     1081 tgcagccgtg gtcgatgatc gcgacgcgct gcgccgcctt ctcggcctcg tccatgtagt
     1141 gcgtcgtcaa gaacaccgta acgccttcgt tcgcgttcaa gtttttcacg tgcgtccaga
     1201 gctggttgcg gctctgcgga tccaagccaa gcgtcggctc gtcgaggaac aggatcttcg
     1261 gcgtgtgcaa caggccgcgc gcgatctcga gcctgcggcg catgccgccc gagaacgtct
     1321 tgacgagcga cgtgcgccgg tcccagagct cgaacagcat cagcagcaac tcgatgcgct
     1381 cggtgcgcag cttgcgcgga acgtgataga gcacgccgtg cagctccatg ttctcgtaag
     1441 ccgtgagctc gccgtcgagg ctcgggtcct ggaacacgat gccgaagcgt ttgcgcacct
     1501 cgagcgggct cactgtcgga tccaagccgt cgatgcgcac gctgccgctc gtcggcttga
     1561 gcagcgtcgt cagcatttga atcgtcgtcg tcttgccggc gccgttcggg cccaagaacg
     1621 cgaagatttc gccgggcttc acgctgaacg tgacgtcctg gacggcgcgc acggccttgt
     1681 ccgatttcgg gtcgccgaaa accttgttca gcgcttgagc ttcgatcaca cgacctccgt
     1741 tcgagccgag cggcggggcg caattatgcg cgcttcgcgc gagctcagtc gaggtaatcg
     1801 gccttcagcg cgctcgaatc gaacagcgcg cgagcgagcg tgagcgcggc ttcgcgttcc
     1861 gcgtcgttct catgctcggc gctccaaatc gccgacagca cggcctgcgc gaagcaccag
     1921 ccgcgcgctc tgccgacgtc gagcccgagc gcgagaccga agcgctcgag ccgccgctcg
     1981 acgacgtcga gcgtcgcgaa caggtccggc cgctcgatcg ggttgcgcag agcggcgccg
     2041 agctcgtact cgagctcgcc gacgacgcct ttgacgtcga tcgcgcacca gccgcgctcg
     2101 cggtccgaca agacattgta gtggtgcaag tcgccgtgca gcagcgccgg gttgcgctgc
     2161 gtcgcggcga ggtccgagta gattcgctgc gccggctcga cgagcgccat cggcacgcgc
     2221 tcgtcgccgc tgccggcgtg acgcacgaac gcgccgcccc attgctcgac gc
//
LOCUS       DASSNN010000528         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_595718, whole genome shotgun sequence.
ACCESSION   DASSNN010000528 DASSNN010000000
VERSION     DASSNN010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595718"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1586
                     /locus_tag="VFL84_09865"
     CDS             <1..1586
                     /locus_tag="VFL84_09865"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HET7608971.1"
                     /translation="RTFGLEGLLPVRAKNQSQQAERIYQQLRAAPDDLQKYVMLNALL
                     NRNVHLYYRVLADHLEELMPIVYTPTVGAAVQRFSQVFQGGGGIWISPDMKGRMQDVL
                     WNIVGSREIRLLVVTDNESILGIGDQGAGGMAISQGKLALYTAAAGIDPAKAIPVSLD
                     FGTDNEQLLADDQYLGWPSRRLRGAEYLALVDEFVAAVRTVMPDALVQWEDFRKDNAL
                     TILDRYRNVLPSFNDDIQGTGAVVMAGLNSALTIKGERLAAQRIVIFGAGAAGLGVAR
                     QIKAQLVDESVVGDAQANVVAVLDRGGLLVHDGRPMDDYKRALAWPAALAAQLGLADP
                     TQRSLLDVVRKYRPTVLIGLSGVARSFNEQIVREMAAHVDRPVIFPSSNPSSISEATP
                     ADLYAWTDCRCLVATGSPFPQVECGGRVFRVGQGNNVFIFPGLGLAAIAANATRITDS
                     MTKVASEALAKQVTSEERDAGLLFPAVSRLRTVSFEIAVAVAHQAIQDGVATAAHDDV
                     ERLVREARWSHDYQDYEPA"
     gene            1598..1984
                     /locus_tag="VFL84_09870"
     CDS             1598..1984
                     /locus_tag="VFL84_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7608972.1"
                     /translation="MTKDEFATYFEALQAHDYDTFKKYYTPDYRAHFDGQSFDREGVI
                     AVERKLAEIADSRWDVLDVVADDNGIAVHAFLEMRFLKDSPPGFALGAFKAGERVKKR
                     FCGFYKLRGGKICEFRVFPFLGERLD"
BASE COUNT          361 a          730 c          702 g          365 t
ORIGIN      
        1 ggcggacgtt cggcctcgag ggcctgctgc cggtgcgcgc gaagaatcaa tcgcagcagg
       61 ccgagcgcat ctatcagcag ctgcgtgccg cgcccgacga tctgcagaag tacgtgatgc
      121 tgaacgcgct gctcaatcgc aacgttcatc tctattacag ggtgctcgcc gaccatctcg
      181 aagagctgat gccgatcgtg tacacgccga cggtcggcgc ggccgtgcag cgcttcagcc
      241 aagtgttcca gggcggcggc ggcatctgga tctctcccga catgaaaggc cgcatgcagg
      301 acgtgctctg gaacatcgtc ggctcgcgcg agattcgctt gctcgtcgtc acggacaacg
      361 agtcgattct cggcatcggc gatcaaggcg cgggcggcat ggcgatctcg caaggcaagc
      421 tcgccttgta cacggcggcg gcgggcatcg atcccgcgaa agcgatcccc gtgagcctcg
      481 acttcggcac cgacaacgag cagctgctcg ccgacgacca gtacttgggc tggccttcgc
      541 gccgcctgcg cggggcggag tatctcgcgc tcgtcgacga gttcgtcgcg gccgttcgca
      601 ccgtgatgcc ggacgcgctc gtgcagtggg aggacttccg taaggacaac gcgctgacga
      661 ttctcgaccg ctaccggaac gtgctgcctt cgttcaacga cgacatccag ggcaccggcg
      721 ccgtcgtgat ggccgggctc aacagcgcgc tgacgatcaa gggtgagcgc ctggccgcgc
      781 agcgcatcgt catcttcggc gccggcgccg cgggactcgg cgttgcgcgg cagatcaagg
      841 cgcagctcgt cgacgaaagc gtcgtcggcg acgcgcaagc caacgtcgtc gccgtgctcg
      901 accgcggtgg cctgctcgtc cacgacggcc ggccgatgga cgactacaaa cgcgcgctcg
      961 cgtggccggc cgcgctggcc gcgcagctcg gcctcgccga cccgacgcag cggagcttgc
     1021 tcgacgtcgt gcgcaagtac cgcccgaccg tgctgatcgg gctttccggc gtggcgcgca
     1081 gcttcaacga gcagatcgtt cgcgagatgg cggcgcacgt cgatcggccc gtgatctttc
     1141 cgtcgtcgaa cccgagctcg atctcagaag cgacgccggc cgatctctac gcctggaccg
     1201 actgccgctg cctcgtcgcg acgggcagcc cgttcccgca agtcgaatgc ggcgggcgcg
     1261 tcttccgcgt cggccagggc aacaatgtct tcatcttccc gggcttagga ttagccgcga
     1321 tcgcggcgaa cgcgactcga atcacggact cgatgacgaa agtcgcgtcg gaagcgctcg
     1381 cgaagcaagt gacgagcgag gagcgcgacg ccggcctgct gttccccgcg gtatcgcgcc
     1441 tgcgcacggt gtcgttcgag atcgccgtcg cggtcgcgca tcaggcgatc caggacggcg
     1501 tcgcaaccgc ggcccacgac gacgtcgagc gcctcgtccg cgaagcgcgc tggtcgcacg
     1561 actaccagga ttacgagccc gcgtagcaag ccagcagatg acgaaggacg agttcgcgac
     1621 ctacttcgag gcgctgcaag cgcacgacta cgacacgttc aagaagtact acacgcccga
     1681 ctatcgcgcg cacttcgacg gccagagctt cgaccgcgag ggcgtgatcg ccgtcgagcg
     1741 caagctcgcg gagatcgccg attcgcgctg ggacgtcctc gacgtggtcg ccgacgacaa
     1801 cggcatcgcc gtgcacgcgt tcctcgagat gcgcttcttg aaggattcgc cgcccggctt
     1861 cgctctcgga gcgttcaaag ccggcgagcg cgtcaagaag cggttttgcg gcttctacaa
     1921 gctgcggggc ggcaagatct gcgagttccg cgtgttcccg ttcctcgggg agcgcctcga
     1981 ctagcgctac gcctcccgat cggcggcctc tttcatatcc agacgaccca cagcgtcttg
     2041 cgcctgcagt cgtagcagta ggcgttcgcg acctcggcaa cggggcgttc ttctttttgc
     2101 cagccgaaca cgatccacat gcgccgattc cttcttctgt tgttcgctcg gagcgcta
//
LOCUS       DASSNN010000529         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1697087, whole genome shotgun sequence.
ACCESSION   DASSNN010000529 DASSNN010000000
VERSION     DASSNN010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697087"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFL84_09875"
     CDS             complement(<1..736)
                     /locus_tag="VFL84_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7608973.1"
                     /translation="MTANARKTVVIAGEFPMHRGGTLLSPTIAFETWGKLNSKRDNAV
                     LIFTGMSPSAHAASSPEDPTPGWWEEIIGPGRPIDTRRYFVIVVNSLGSCFGSTGPAS
                     VDPRTGDIYRLTFPVLSLEDVARGGLEVIKHLGVNRLVATVGPSMGGMTALAFEILFP
                     GISDGFVSMSSGARSLPFSIALRSTQREMIRKDPAWMGGNYPRDAQPLVGMRLARKLG
                     MITYRSAEEWRLRFGRERAAVHASANG"
     gene            complement(850..1143)
                     /locus_tag="VFL84_09880"
     CDS             complement(850..1143)
                     /locus_tag="VFL84_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608974.1"
                     /translation="MKPAEKIAWAVGGVGFVAAAIIGLDERPADGGGLVIALASYAVG
                     LHWIIAYLFGGTMYGALRLQPTHRLARLAVLALGVLLTFLALQYVLGFGGLFA"
     gene            complement(1140..>2094)
                     /gene="der"
                     /locus_tag="VFL84_09885"
     CDS             complement(1140..>2094)
                     /gene="der"
                     /locus_tag="VFL84_09885"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET7608975.1"
                     /translation="GQGVDALLRAALASFPAADETDPALETEQGPKVAVIGRPNVGKS
                     TLINRLLGENRLITSPEPGTTRDSIHVPVERDDKKFVLVDTAGIRRRARVSLTIEKYS
                     VVQSLKAIEEAGAVIAVLDARENVTDQDVHLVGLAAERGRALALAINKWDGLASHERR
                     FVEREVERKLDFVPYASRHYISALHGSGIAELIKAALVGYKNAGVELATPRLTRVLED
                     ATQANPPPLVRGRQVRLRYAHQGGRYPPLIVVHGTQAQRLPDHYKRYLENAFREALRL
                     KGTPVRIELRTTENPFAGRRNKLTPRQAKHRRRMLRFKHKE"
BASE COUNT          339 a          712 c          714 g          329 t
ORIGIN      
        1 cgccgttcgc ggaggcatgc accgccgcgc gctcgcgccc gaagcgcagc cgccattcct
       61 cggccgagcg gtaggtgatc atgccgagct tgcgcgcgag ccgcatgccg acgagcggtt
      121 gcgcgtcgcg cggatagttg ccgcccatcc acgccgggtc cttgcggatc atctcgcgct
      181 gcgtcgagcg cagcgcaatc gagaacggca acgagcgagc gccggacgac atgctgacga
      241 agccgtccga gatgcccgga aacaggatct cgaatgcgag cgccgtcatg ccgcccatcg
      301 acggccccac ggtcgcgacg agccgattca cgccgagatg cttgatgacc tcgagcccgc
      361 cgcgcgcgac gtcctcgagc gagaggacgg ggaacgtcag gcgatagatg tcgcccgtgc
      421 gcgggtcgac ggacgcgggg cccgtcgagc cgaagcagct gccgagcgag ttcacgacga
      481 tcacgaagta gcggcgcgtg tcgatcggcc gcccggggcc gatgatctcc tcccaccacc
      541 cgggcgtcgg atcctcgggg ctcgacgccg cgtgcgccga cggcgacatg cccgtgaaga
      601 ttagcaccgc gttgtcgcgc tttgagttca gcttgcccca ggtctcgaac gcgatcgtcg
      661 gagacagcaa agtgccgccg cggtgcatag gaaactcacc ggcaatgacg accgtcttgc
      721 gcgcgttcgc cgtcacgctt agcccccaag ttcgagataa gaggcagtct agaacccgcg
      781 ggatgcgagg cttctaactt tcggctatgt aattaggccg cgcgcgcggg gtatgccgag
      841 ccggcactct caggcaaaca atccgccgaa cccgagcacg tattgcagag cgaggaacgt
      901 tagcaagacc ccgagggcga ggacggcaag gcgcgccaaa cgatgcgtgg gctgcaagcg
      961 cagcgcgccg tacatcgtgc cgccgaacag atacgcgatg atccagtgca aaccgacggc
     1021 gtagctcgcc aatgcgatga cgagaccgcc gccatcggcg ggccgctcgt cgagcccgat
     1081 gatggccgcg gcgacgaagc cgacaccgcc tacggcccac gcaatcttct cggccggttt
     1141 cattctttgt gcttgaaccg cagcatgcgg cggcggtgct tcgcttggcg cggcgtcagc
     1201 ttgttgcgcc ggcccgcgaa cgggttctcg gtcgtgcgca gctcgatgcg cacgggcgtg
     1261 cccttcagcc gcaacgcctc gcgaaacgcg ttctcgagat agcgcttgta atggtcgggc
     1321 aagcgctgcg cctgcgtgcc gtgcacgacg atcagcggcg ggtagcgccc gccttgatgc
     1381 gcgtagcgca gccgcacctg gcgcccgcga acgagcggcg gcggattcgc ttgcgtcgcg
     1441 tcctcgagca cgcgcgtcaa gcgcggcgtc gcgagctcga cgccggcgtt cttgtagccg
     1501 acgagcgcgg ctttgatcag ctccgcgatg ccgctgccgt gcagcgccga gatgtaatgc
     1561 cggctcgcgt acggcacgaa gtcgagcttg cgctcgacct cgcgctcgac gaagcggcgc
     1621 tcgtggctgg caagaccgtc ccacttgttg atcgcgagcg cgagcgcgcg gccgcgctcg
     1681 gccgcgagcc cgaccaagtg cacgtcctgg tcggtaacgt tctcgcgcgc gtcgagaacg
     1741 gcgatgacgg cgcctgcttc ctcgatcgcc ttcaggctct gcacgacgct gtacttctcg
     1801 atcgtcaagc tgacgcgcgc gcggcggcgt atgccggccg tgtctacgag cacgaatttt
     1861 ttgtcgtcgc gctcgacggg cacgtggatg ctgtcgcgcg tcgtgccggg ctcgggcgac
     1921 gtgatcaagc ggttctcgcc gagcagccgg ttgatcaacg tcgacttgcc gacgttcggc
     1981 cggccgatca cggcgacttt cgggccctgc tcggtctcca aggccgggtc ggtctcgtcc
     2041 gcggccggaa acgaggcgag cgccgcgcgc agcagcgcgt cgacgccttg cccg
//
LOCUS       DASSNN010000530         2729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_683320, whole genome shotgun sequence.
ACCESSION   DASSNN010000530 DASSNN010000000
VERSION     DASSNN010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683320"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VFL84_09890"
     CDS             complement(<1..1005)
                     /locus_tag="VFL84_09890"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET7608976.1"
                     /translation="MTEAADSGRKAIVTIEDRNRRHELGAADLPVTLGASHDADVTLE
                     GVAGSIQIGRFKDAFFVQSGRGARNLRVGGEPLSGTRELVDGDVIAFDRARLTCRVAN
                     GKLALKVDWIVTAGDTAPPDLDQVVRDRSRAADVAITPIAFKPSAAAAKAAPKPGLSR
                     MQIVLASGTAVLAIVAWFAFTAKSVALNIEPTPSTVTLPSTLFKLKIGERFLLRPGTH
                     RVAAELEGYYPLDTKIDVGSHDDQTIELTLTKLPGLVTLTTDPEVGAEVLVDGVAVGK
                     TPLVDAEITPGAHQLELSAERYLSAARELDVAGGGERQSLAVTLTPDWAVVSLNTAPA
                     G"
     gene            complement(1023..2528)
                     /locus_tag="VFL84_09895"
     CDS             complement(1023..2528)
                     /locus_tag="VFL84_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608977.1"
                     /translation="MEPNVPQHDETIVPVRPNLRGAAVAPRASVEAGSRLVRVSLTAL
                     GAVALGLAAAAAFYWLPASVDEERAAAAARPAEPTPDVPARPVLTPEQAAALKAQSGD
                     LLAGLLTLTDELEQLNVEAWAAEDWAKYQQLSEAGDNAFLADDFSTSAASYAEAKALG
                     DALKTRAATTITDSLAAAEAAVAAGDSELALQKYDVVLTIEPTNAKAAAGRARAERLP
                     EVISLVQRGNVEVARGELETALATYREALAIDADWPAAREGVDDVNRMLRDAEYERRL
                     SAGFGLLTSEDFGDARREFEAALALRPGSREATDGIAQAVEGAKLSQIKLAEARALAF
                     ERRELWTQAIELYRDVLAGDGTLLFAQTGLERAQRRAGLDAKLANLIDNPTLLFGDQV
                     LADARALLETAGAEAEKGPRLESQIKKLGELVELASKPLPVRLESDQLTTVTLYRVGT
                     LGAFAAKDLELRPGTYTVIGSRDGYRDVRRTFTVRPGRSLAPISVVCVEPI"
     gene            complement(2533..>2729)
                     /locus_tag="VFL84_09900"
     CDS             complement(2533..>2729)
                     /locus_tag="VFL84_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608978.1"
                     /translation="TTARLPYGPRGAAAEGAELAAAASADAAAARLAGAAIEALRARG
                     RVGGLSYFSDVIESVRGQHG"
BASE COUNT          387 a          971 c          948 g          423 t
ORIGIN      
        1 gccggccggt gcggtgttca gcgacacgac ggcccagtcc ggcgtcagcg tcacggcgag
       61 cgactggcgc tcaccgccgc cggcgacgtc gagctcgcgc gcggcggaga ggtagcgctc
      121 ggccgagagc tcgagctggt gggctccggg cgtgatctcg gcgtcgacga gcggcgtctt
      181 gccgaccgct acgccgtcga cgagcacctc ggcgccgacc tccgggtcgg tcgtcagagt
      241 cacgaggccc ggcagcttcg tcagcgtcaa ttcgatcgtc tggtcgtcgt gcgaaccgac
      301 gtcgatctta gtgtcgagcg ggtagtagcc ctcgagctcc gcggcgacgc ggtgcgtgcc
      361 cgggcgcagc aggaaccgct cgccaatctt gagcttgaac agagtgctcg gcagggtgac
      421 ggtcgaaggc gtcggctcga tgttcaacgc gacggacttc gccgtgaacg cgaaccacgc
      481 gacgatggcc agcaccgcgg tgccgctcgc gagcacgatt tgcatgcggc tcaaaccggg
      541 tttcggcgcg gccttcgccg cggcggcgga cggcttgaaa gcgatcggcg tgatcgcgac
      601 gtcggccgct cggctccggt cgcgcacgac ctggtccagg tcgggcggcg ccgtatcgcc
      661 ggccgtgacg atccaatcca ctttgagcgc aagcttgccg ttcgcgacgc gacacgtcag
      721 gcgcgcacga tcgaacgcga tgacgtcgcc gtcgacgagc tcgcgcgtgc cgctgagcgg
      781 ctcgccgccg acgcgcagat tgcgcgcacc tcggccggac tgcacgaaga acgcgtcctt
      841 gaagcggccg atctggatgc tgccggcgac gccttcgagc gtgacgtcgg cgtcgtgtga
      901 cgctccgagc gtgacgggca gatccgcggc gccgagctcg tgccgccggt ttcggtcctc
      961 gatcgtcacg atcgcttttc gcccgctgtc tgctgcctct gtcactcgaa tgtcctgtcg
     1021 ctctagatgg gctcgacgca gaccacgctg atcggcgcca agctccggcc cggccgcacg
     1081 gtgaaggtcc ggcgcacgtc gcgatagccg tcgcggctgc cgatgacggt gtaagttcct
     1141 gggcgcagct cgaggtcctt cgcggcgaat gcgccgagcg tgccgacgcg atacagcgtc
     1201 acggtcgtca gctggtccga ctcgagccgc acgggaagcg gcttcgacgc gagctcgacg
     1261 agctcgccga gcttcttgat ttgcgactcg aggcgcggcc ccttctcggc ctcggcgccg
     1321 gccgtctcga gcaacgcgcg cgcatcggcg agcacctgat cgccgaacag cagcgtggga
     1381 ttgtcgatga gattcgcgag cttcgcgtcg aggcccgctc gtcgttgcgc acgctcgaga
     1441 cccgtttgcg cgaacagcaa agtgccgtcg ccggcgagca catcgcgata gagctcgatc
     1501 gcttgcgtcc agagctcgcg ccgctcgaac gcgagcgcac gggcttcggc cagcttgatc
     1561 tgactgagct ttgcgccctc cacggcttgg gcgataccgt cggtcgcttc gcgcgagccg
     1621 ggccgcagag cgagcgccgc ttcgaactcg cgccgcgcgt cgccgaagtc ctccgacgtg
     1681 agcaagccga agccggccga caaccgccgc tcgtattcgg cgtcgcgcag catgcgattg
     1741 acgtcgtcga caccctcgcg cgcagccggc cagtcggcgt cgatcgcgag cgcctcgcga
     1801 tacgtcgcga gcgcggtctc gagctcgccg cgggcgactt cgacgttccc gcgctgaacg
     1861 agtgagatga cctcgggcaa ccgctcggca cgcgcgcgtc cggccgccgc cttcgcgttc
     1921 gtcggctcga tcgtcagcac gacgtcgtac ttctgcagcg cgagctccga atcgccggcg
     1981 gccacggcgg cctccgccgc ggcgagcgag tccgtgatcg tcgtcgccgc gcgcgtcttc
     2041 agcgcgtcgc cgagggcctt ggcttcggcg tagctcgcgg cggaagtcga aaaatcgtcg
     2101 gcgagaaacg cgttgtcgcc ggcttcggat agctgctggt acttcgccca gtcctcggcg
     2161 gcccaggcct cgacgttcag ctgctcgagc tcgtccgtca acgtcaagag acccgcgagc
     2221 aagtcgcccg attgggcctt cagcgccgcg gcctgctccg gcgtcaaaac cggccgcgcc
     2281 ggcacatcgg gcgtcggctc cgcggggcgt gccgctgcag cggcgcgttc ctcgtcgacg
     2341 ctcgccggca gccagtagaa ggcggccgcc gcggcgagcc ccaaggccac ggccccgagc
     2401 gccgtcagcg agacgcggac gagccgcgag ccggcttcga cgctcgcccg cggggcgact
     2461 gccgcgccgc gcaaattggg ccggaccgga acgatcgttt cgtcgtgttg tgggacgttt
     2521 ggttccaaag ccttaaccgt gctggccgcg cacagattct atgacatcgc tgaaatacga
     2581 aagtcctcca acgcggccgc gggcccgcag cgcctcgatg gccgcccccg cgagccgcgc
     2641 tgcagcggcg tccgccgacg cagcggcggc gagctcggcg ccttccgccg ccgcgccgcg
     2701 cggcccatag ggcaaccgcg ccgtcgtga
//
LOCUS       DASSNN010000531         3543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1839706, whole genome shotgun sequence.
ACCESSION   DASSNN010000531 DASSNN010000000
VERSION     DASSNN010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839706"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFL84_09905"
     CDS             <1..783
                     /locus_tag="VFL84_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET7608979.1"
                     /translation="VEQIDPFLFLNHHGPQVYAPRNRGLPFGPHPHRGFETVTFILTG
                     ELAHRDSAGHESVIKEGGIQWMTAGSGLVHAEISPEEFMRKGGPLEILQLWVNLPPRL
                     KMTPPRYVGLQRDEIPAIATPDGNGKVNLVSGEYLGRKGAMRPLLDIFMSTVELKSGA
                     RVEFPGVAQRNVFLYVVRGAVEVAGEKAPEWHLIELGRAGDSVVIDAKSDAVLLFGHA
                     EPIGAPVVSYGPFVMNTREEIMQAIHDYQAGKFNAVPLGSGA"
     gene            789..1400
                     /locus_tag="VFL84_09910"
     CDS             789..1400
                     /locus_tag="VFL84_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET7608980.1"
                     /translation="MARTKVRNVVFDFGGVLVSWRPQEIIDSFYAEQQLRDALRMHAF
                     QHDDWLDMDRGTLDEASVVRRCAARMARPESELEALFDHLRAALTPIEPTVALLRELR
                     RRDGLKLYGLSNMSEAIFAYLDGRHDFFKLFDGIVVSAAVKLLKPEAAIYEHLRDRFA
                     LNFAETVFIDDMERNVESARRVGLPAIQFVTTEQVRRELEPLL"
     gene            1448..2230
                     /locus_tag="VFL84_09915"
     CDS             1448..2230
                     /locus_tag="VFL84_09915"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET7608981.1"
                     /translation="MRTKLLMVALMACLLGGVASAQFFGGRGGREAPEPRGAPPEAEF
                     HMARLAYYARGCAGSRGYCNPWWAIDYPYAEAHFLPAVERMTRVEVAPDSRHITIDDE
                     YLFDYPWLFLQQPGQGNWHPQGEELERLREYLERGGFLVVDDFHSESEWRSVADAMQD
                     LLPGRPIVDIPEDDPLHHILFDLDKRTQIPGERHLGWRSMEGPPHWRGIYDDHGRLMV
                     ALNHNSDMGDAWEHADDAHYPAPMTATAYRFGVNYVIYAMTH"
     gene            2232..2684
                     /locus_tag="VFL84_09920"
     CDS             2232..2684
                     /locus_tag="VFL84_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET7608982.1"
                     /translation="MRRVLYLDDLAVGRKFATGSVTLTLEGCKAFAAEFDPQPFHLDE
                     KAARESVFGRIAASGWYTAALSMRLLVEGELTIAGGLIGLGGEVTWPRPTYPGDTLRV
                     ETEVLAIRVSESKPDRGIVTARNRTLNQHGEPVQIAVVKMLVPRRPGA"
     gene            complement(2689..>3543)
                     /locus_tag="VFL84_09925"
     CDS             complement(2689..>3543)
                     /locus_tag="VFL84_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608983.1"
                     /translation="DPDVGLNDDVIDGRFHSNTGVTINRTGRATPVFGGKVTIAGGEV
                     VTMAEGFLSSHPARLNKRKLFPAGLETHARRIELPERATALEAIPGPDNSQRFARDTA
                     LTFHDDGSVSWRELDGGAAGARRALGGEPFYLLADEDVELEVSGTVNGKVLVYTPDRI
                     VVTGDLRYATDPSAPQADDYLGLVAERTVEIAEPEITGPGDLEIFASIYARQRFVVRD
                     YLSRRSGTLIVHGSLTAGTLSASEPRFATRVEFDERLTTMRAPGFPLSDRYELDSSSG
                     EWRVVDTP"
BASE COUNT          557 a         1187 c         1215 g          584 t
ORIGIN      
        1 gtcgagcaga tcgacccgtt cttgttcttg aaccaccacg gtccgcaggt ctacgcgccg
       61 cgcaatcgcg gactgccgtt cggcccgcat ccgcaccgcg gtttcgagac cgtgacgttc
      121 atcctgacgg gcgagctcgc gcaccgcgac agcgcgggac acgagagcgt catcaaggag
      181 ggcggcattc agtggatgac ggccggcagc ggcctcgtgc acgccgagat ctcgcccgag
      241 gagttcatgc gcaaaggcgg gccgctcgag atcctgcagc tctgggtcaa cttgccgccg
      301 cggctcaaga tgacgccgcc gcgctacgtc gggttgcagc gcgacgagat tcccgcgatc
      361 gcgacgccgg acgggaacgg caaggtcaac ctcgtctcgg gtgagtatct gggccgcaaa
      421 ggcgcgatgc ggccgctgct cgacattttc atgtccaccg tcgagctcaa gtccggcgcg
      481 cgcgtcgagt ttcccggcgt cgcgcagcgc aacgtgttcc tctacgtcgt gcgcggcgcg
      541 gtcgaagtgg ccggcgagaa ggcgcccgag tggcatctga tcgagcttgg gcgtgccggc
      601 gattccgtcg tcatcgacgc gaagtccgac gccgtgctgt tgttcggcca cgccgagccg
      661 atcggcgcac cggtcgtctc gtacggcccg ttcgtcatga acacgcgcga agagatcatg
      721 caggcgatcc acgactacca ggccggaaag ttcaacgcgg ttccgttggg aagcggcgct
      781 tgacgagcgt ggcgcgcacg aaggtgcgca acgtcgtctt cgacttcggc ggcgtgctcg
      841 tgagctggcg cccgcaggag atcatcgact cgttctacgc ggagcagcag ctgcgcgacg
      901 ccttgcgcat gcacgccttc cagcacgacg actggctcga catggaccgc ggcacgctcg
      961 acgaggccag cgtcgtgcgc cgctgcgccg cgcgcatggc gcgcccggag agcgagctcg
     1021 aagcgttgtt cgaccacctg cgagcggcgc tgacgccgat cgagccgacc gttgcgctgc
     1081 tgcgcgagct gcgccggcgc gacgggctca agctctacgg cctctcgaac atgtcggaag
     1141 cgatcttcgc ttacctcgac gggcggcacg acttcttcaa gctgttcgac ggcatcgtcg
     1201 tatcggccgc cgtcaagctg ctgaagcccg aggccgcaat ctacgagcac cttcgcgacc
     1261 gtttcgcgct gaatttcgcc gagaccgtgt tcatcgacga catggagcgc aacgtcgaga
     1321 gcgcgcggcg cgtcggcttg ccggcgattc agttcgtgac gaccgagcag gtgcgccgcg
     1381 agctcgagcc cctgttgtag gggagcgcct ggcggtccgc tgcccggggt ggcacagtac
     1441 gaggcggatg cgtacgaagt tgctgatggt cgcgctcatg gcctgcctgc ttggcggagt
     1501 cgcgagcgcg cagttcttcg gcggccgcgg cggccgtgag gcgcccgagc cgcgcggcgc
     1561 gcctcccgag gccgagttcc acatggcgcg gctcgcctat tacgcgcgcg gctgcgcggg
     1621 ctcgcgcggc tattgcaacc cgtggtgggc catcgactac ccttacgcgg aggcgcactt
     1681 cttgcccgcc gtcgagcgca tgacgcgcgt cgaggtcgcg cccgacagcc gccacattac
     1741 gatcgacgac gagtacctgt tcgactaccc gtggctgttc ctacagcagc cggggcaggg
     1801 caactggcac ccgcaaggcg aggagctcga gcggctgcgc gagtacctcg agcgcggcgg
     1861 cttcctcgta gtcgacgact ttcacagcga gtccgaatgg cggtcggtcg ccgacgcgat
     1921 gcaagacctt ttgcccgggc gtccgatcgt cgacatcccc gaggacgacc ctctgcacca
     1981 cattctgttc gatctcgaca agcgcacgca gataccaggc gagcggcatc tgggctggcg
     2041 ctccatggaa ggccccccgc actggcgcgg catctacgac gatcatggcc gcctcatggt
     2101 cgcgctgaat cacaacagcg acatgggcga cgcgtgggag cacgccgacg acgcgcatta
     2161 ccccgcgccg atgacggcaa ccgcgtaccg cttcggcgtc aactacgtca tctacgcgat
     2221 gacgcattga gatgcgtcgg gtcctctatc tcgacgatct tgcggtcggc cgcaagttcg
     2281 cgacgggctc ggtgacgctg acgctcgaag ggtgcaaggc cttcgccgcc gagttcgatc
     2341 cgcagccgtt ccatctcgac gagaaggccg cgcgcgagag cgtgttcggc cgcatcgccg
     2401 cgagcggctg gtatacggcg gcgctgtcga tgcgcttgct cgtcgaaggg gagctgacga
     2461 tcgcgggcgg cctcatcggc ctcggcggcg aggtaacgtg gccgcgcccg acgtatcccg
     2521 gcgacacgct gcgcgtcgag acggaagtgc tcgcgattcg cgtctccgaa tcgaagcccg
     2581 atcgcggcat cgtcacggcg cgcaaccgga cactgaatca gcacggcgag cccgtgcaga
     2641 tcgcggtcgt caagatgctc gtgccgcggc ggcccggcgc ttaagctttc acggggtgtc
     2701 gacgacgcgc cattcgccgc tcgaggagtc gagctcatag cggtcgctca gcgggaagcc
     2761 gggcgctcgc atcgtcgtca gtcgctcgtc gaattcgacg cgcgtcgcga agcgcggctc
     2821 gctcgcgctc agcgtgccgg ccgtcaagct gccgtgcacg atcaacgtgc cgctgcgccg
     2881 cgacaagtaa tcgcggacga cgaagcgttg ccgagcgtag atcgaagcga agatctcgag
     2941 atcgccgggg ccagtgatct ccggctctgc gatctcgacg gtgcgctccg cgacgagccc
     3001 taaatagtcg tcggcctgcg gcgcgctcgg gtcggtggcg tagcgcagat cgccggtgac
     3061 gacgatgcgg tccggcgtgt acacgagcac tttgccgttg acggtgccgc tgacctcgag
     3121 ctcgacgtcc tcgtcggcga gcaagtagaa cggctcgccg ccgagcgcgc ggcgcgctcc
     3181 cgcggctccg ccgtcgagct cgcgccagct cacgctgccg tcgtcatgaa aggtcagcgc
     3241 ggtgtcgcgt gcgaagcgct ggctgttgtc ggggccggga atcgcctcga gtgccgtcgc
     3301 gcgctcgggc agctcgatgc ggcgggcgtg cgtctcgagg ccggccggaa acagcttgcg
     3361 cttgttcaag cgggccggat gcgacgacag aaatccctcg gccatcgtga cgacttcgcc
     3421 gccggcgatc gtgactttgc cgccgaagac cggcgtcgcc cgaccggtac gattgatcgt
     3481 cacgcccgtg ttggaatgga atcgtccgtc gatgacgtcg tcgttgagcc cgacgtcggg
     3541 atc
//
LOCUS       DASSNN010000532         3750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1697393, whole genome shotgun sequence.
ACCESSION   DASSNN010000532 DASSNN010000000
VERSION     DASSNN010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697393"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VFL84_09930"
     CDS             complement(<1..1261)
                     /locus_tag="VFL84_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019609773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET7608984.1"
                     /translation="MYRVLPALTLLLCWGGGWAQPPDTNRNPHWVGTWGAAQQLAAET
                     LPPWVVPPPREPNEPPPRALLPPYPTSFNDETLRMVVRTSIGGERLRLTLSNALGRSE
                     VHIGAVHIALRERGSAIAVATDRAVTFGGKASFAVQPGTLVVSDTIELAVPPLAELAV
                     SIYLPDETLDVTTHEFALNTTYVVSGNAAAAASLTNPRTNFTYFWLNGIEVLAAADAG
                     AIVALGDSITDGVTTTFDAQRAWPALLAAKLQANPATRNWAVINAGISGNRVRRDVIG
                     RSALGRFDRDVLARAGVEWLVLFEGINDVTWTALPAVPDGERATADEIIETLEQIVDR
                     AHARGIKVIGGTLMPMGGLWLHKAETEAMRQAVNEWIRTSGKLDAVADFDAATRDPNQ
                     PARLLSQVDSGDHIHPNDAGNAAMAGAI"
     gene            complement(1276..2361)
                     /locus_tag="VFL84_09935"
     CDS             complement(1276..2361)
                     /locus_tag="VFL84_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HET7608985.1"
                     /translation="MHRRLLFGRSLALAHLAVLCGGALAQQASPPKVAVEPDGTVVMP
                     AQSVPVSGFLSAEGKAYLAEHLRNLQRPEMLVQENGVPRLIAGYLARQREVFAVERAE
                     TRVGGVHAYVYTPRDGIAAENRHRVLVDLHGGGFRECWPACAELESIPIAALGRITVV
                     SLDYRQGPEHRHPAASEDVAAAYRELLKSYRPENIGLYGCSAGGMLAGMAVAWFQRHG
                     LPRPGAVGILCAGAANLEGNAFAGDAGYTTLPLGEGRAPPPGGNAVRMEYFAGTDPDD
                     PLVAPAASPGVLAKFPPTLIVTGTRSFDLSNAVYTHAQLVKNGVDADLHVWEGMFHGF
                     FYNPDVPESRECYDVIVRFFAKHLGRR"
     gene            2478..3548
                     /locus_tag="VFL84_09940"
     CDS             2478..3548
                     /locus_tag="VFL84_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7608986.1"
                     /translation="MHESYRRPLVTLLVAVCAVATGPLGAQQAAGRIERIRVPGPSLE
                     GNLEGDDATRDVHVYLPPSYSSDSRRRYPVVVFLHGYAATAEAYVRTLDLPQAADRAI
                     AAGAREMIIVLPDAFTGYSGSMYSSSVTTGDWESYIAQDLIAYIDAHYRTLAQRESRG
                     LAGHSMGGYGTVRIGMKRPDVFGALYAMSSCCLLIDPARGGDVVMRAARERAAARGQT
                     AEQPPSGRPDPSAGFANALSAQAAAWAPNPDNPPQYFDLPFKNGELDPLVASKWLANA
                     PLLMVDQYVPSLKRYRAIALDVGNADPLREDNANLDAALTRLRVAHGFEEYDGDHGNR
                     VRERFASKVLPFSSQHLATSSR"
BASE COUNT          616 a         1266 c         1256 g          612 t
ORIGIN      
        1 cgatggcgcc tgccatcgcg gcgttgccgg cgtcgttcgg atggatgtgg tcgccgctgt
       61 cgacttgcga cagcagccgc gccggttggt tcgggtcgcg cgtggccgcg tcgaaatccg
      121 ccactgcatc gagcttgccg ctcgtgcgaa tccattcgtt gacggcctga cgcatcgcct
      181 ccgtctcggc cttgtggagc cagagtccgc ccatcggcat cagcgttccg ccgatgacct
      241 tgatgcctcg agcgtgggcg cggtcgacga tctgctcgag cgtttcgatg atctcgtcgg
      301 ccgtcgctcg ctcgccgtcc ggtacggctg gcagagcagt ccacgtcacg tcattgatgc
      361 cctcgaacag cacgagccac tcgacgcccg cgcgcgccag cacgtcgcga tcgaaccggc
      421 cgagcgcgct cctgccgatg acgtcgcgcc gtactcggtt gccggagatc ccggcgttga
      481 tgacggccca gttgcgagtc gcgggattcg cctgaagctt cgcggcgagg agcgccggcc
      541 acgcgcgctg cgcatcgaac gtcgtcgtga cgccgtcggt gatcgaatcg ccgagcgcca
      601 cgatcgcgcc tgcgtcggcg gcggcgagca cctcgatgcc gtttagccag aagtaagtga
      661 aattcgtgcg cgggttcgtc agcgatgcgg ccgccgcggc gttgcccgag acgacgtaag
      721 tggtgttgag cgcaaactcg tgcgtcgtca cgtcgagcgt ctcatcaggc agataaatcg
      781 aaacggcgag ctccgcgagc ggcggcacgg cgagctcgat cgtgtcgctg acgacgagag
      841 tgcccggctg aaccgcaaac gacgccttgc cgccgaacgt gacggcacga tccgtcgcca
      901 cggcgatcgc ggagccgcgc tcgcgcagcg caatgtgcac ggcaccgatg tggacctcgc
      961 tccggccgag cgcgttcgac agcgtcaagc gcaaacgctc gccgccgatg ctcgtgcgaa
     1021 cgaccatgcg cagcgtttcg tcattgaaag acgtgggata cggcggaagc agcgcgcgcg
     1081 gaggcggctc gttcggctcg cgcggcggcg gcacgaccca cggaggcagc gtctcggcgg
     1141 cgagctgctg tgccgcgccc caagtgccga cccagtgcgg attccggttt gtgtccggcg
     1201 gctgggccca gccaccgccc cagcacaaca gcagggtgag cgccgggaga acccgataca
     1261 tgcggacgcc tcttctcatc gcctgccgag atgtttggcg aagaaacgca cgatcacgtc
     1321 gtagcactcg cgcgattccg gcacgtcggg gttgtagaag aagccgtgga acatgccttc
     1381 ccagacgtgc aaatcggcat cgacgccgtt cttgacgagc tgtgcgtgag tgtagacggc
     1441 gttgctcaag tcgaaggagc gcgttcccgt cacgatcagc gtgggcggaa acttcgcgag
     1501 cactccgggt gaggcggccg gcgcgacgag cggatcgtcg ggatcggtgc cggcgaagta
     1561 ttccatccgg accgcgttac cgccgggcgg aggcgcgcgg ccctcgccga gcggcagcgt
     1621 cgtgtagccc gcatcgccgg cgaaggcatt tccttccaga ttggccgcgc cggcgcacag
     1681 aatgccgacc gctccgggcc gcggcagccc atggcgctga aaccacgcga ccgccatccc
     1741 ggcgagcatg ccgccggccg agcagccgta gaggccgatg ttctcggggc gatagctctt
     1801 aagaagctcg cggtacgccg cggcgacgtc ctcgctggcg gccgggtgac gatgctcggg
     1861 tccttggcgg tagtcgaggc tcacgaccgt gatgcgcccg agcgctgcga tgggaatcga
     1921 ctcgagctcc gcacaggcgg gccagcactc gcgaaagccg ccgccatgca gatcgacgag
     1981 cacgcggtgg cgattctcgg ccgcgatgcc gtctcgaggc gtatagacgt acgcgtgcac
     2041 gccgccgacc ctcgtctcgg cacgctcgac ggcgaacact tcgcgctgcc gcgcgaggta
     2101 gcccgcgatg agccgcggca cgccgttttc ctggacgagc atctcgggcc gctgcagatt
     2161 gcgtagatgc tcggccaaat acgctttccc ttcggcactc aagaagccgg acacgggtac
     2221 cgactgtgcc ggcattacca ctgtgccgtc gggctcgacc gcgaccttgg gcggcgaggc
     2281 ttgttgagca agcgcccctc cgcacagcac cgccaagtgg gccagagcga gtgaacgtcc
     2341 gaacagcaag cgccggtgca tcgacaactc cgctcccctg cagccgcgta acagccgcga
     2401 aagtaccgcc gggtgcaatc gattgcaaac ctcgctgcgg tcactatcat cggcgtctca
     2461 cgggtcggag ggatgccatg cacgagtcat atcgccggcc tctcgtcaca ctgctcgtcg
     2521 cggtctgtgc tgtcgcaacc ggcccgctcg gcgcacaaca ggccgcaggg cgtatcgagc
     2581 ggatccgcgt gcccggcccg tcgctcgaag gcaacctcga gggcgacgac gcgacacgtg
     2641 acgttcatgt ctatctgccg ccgagctact cgagcgactc tcggcgccgc tatccggtcg
     2701 tcgtcttcct gcacggctac gccgccaccg cggaagccta cgtgcgtact ctcgatttgc
     2761 cgcaggcggc ggatcgcgcg atcgcggcgg gcgcgcgcga gatgatcatc gtgctgcccg
     2821 acgccttcac gggttacagc ggcagcatgt actcgagctc cgtcacgacg ggcgattggg
     2881 agagctacat cgcgcaggat ctgatcgctt acatcgacgc gcactaccgg acgctcgcgc
     2941 agcgggagag ccgcggtctc gcggggcatt cgatgggcgg ctacggcacc gtccgcatcg
     3001 gcatgaagcg ccccgatgtg ttcggtgcgc tctatgcgat gagctcgtgc tgtttgctga
     3061 tcgaccccgc gcgcggcggc gatgtggtca tgcgggccgc gcgtgagcgt gcggcggcac
     3121 gggggcaaac ggcggagcaa ccgccgagcg ggcgtcccga tccatcggcg ggtttcgcga
     3181 acgcgttgtc cgcgcaagcg gccgcttggg ccccgaatcc cgacaatccg ccgcagtact
     3241 tcgatctgcc gttcaagaac ggcgagctcg atccgctggt cgcatccaaa tggctggcga
     3301 atgcgccgct gctgatggtc gatcagtacg tgccgagctt gaagcgttat cgtgccatcg
     3361 cgctcgacgt cggcaacgcc gatccgctgc gcgaggacaa cgcgaacctc gacgccgcgt
     3421 tgacgaggct gcgtgtagcg cacggcttcg aagagtacga cggcgaccac ggcaaccgcg
     3481 ttcgcgagcg gttcgcttcg aaggtgctgc cgttctcctc gcagcatctc gccacgagct
     3541 cccgctagca cgatggcgac gaccgacagc tcccgccgaa cttgagcgga acgatcagac
     3601 cttgaacctc gggaacagcc gctcggcgtt cccgcgcagc atcatccgtt cgaattcttt
     3661 tggcagcgac agccccggca gctcattcac cgtcgactcg atcggctccg gcgagtagtc
     3721 ggtgccgaac aggatctgcg attccggcat
//
LOCUS       DASSNN010000533         3032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_956227, whole genome shotgun sequence.
ACCESSION   DASSNN010000533 DASSNN010000000
VERSION     DASSNN010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956227"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFL84_09945"
     CDS             complement(<1..571)
                     /locus_tag="VFL84_09945"
                     /inference="COORDINATES: protein motif:HMM:NF025760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM domain-containing protein"
                     /protein_id="HET7608987.1"
                     /translation="MGGHVASVDRQAREPKRAPWHGLVRRKGHIDPLLLGALIVIGCI
                     PLLMALARVLALPGADVPAGAEWLRELGRLLNQSFSLRWVMADDRPTALYLLTLPTGA
                     LLVAFARLTLGLRVLGLRAILIAIGFQEIGFLASFGLMIVVVAAIVALRPSMRRIRLP
                     LYARLPIILCLVATIMVGALLFGPWARSET"
     gene            complement(578..1837)
                     /locus_tag="VFL84_09950"
     CDS             complement(578..1837)
                     /locus_tag="VFL84_09950"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET7608988.1"
                     /translation="MANSDKPRRRRHGSPRDRSLVVLKESRPARREEPNRPVVRSLQR
                     RGVALRLVTLATTRLPLARRYAVRFGAFGALALLAAPIVAWLHYQSVHVTSMNAAVRG
                     HIAELGTPLTGLVASVEVDAGDRVAAGQVLVRLDDRTFRAEVNEAAAELEGLQRKIDV
                     ERAAIAHELLQVEQEEQGALANVAAARAQNSAAESQAQNARRLHEARQSLAASGVISN
                     QDLLNAETGSRTAQALVQEARAKQLAALSAEESARLTRDGLAIRERGIGVLEAEVQRA
                     RARLAKAEADLDSASVRAPEAGGILRRIIQPGASVEVGQPLMSMWLGREVWVDAWIDE
                     DDISSVRVGDPATVSLRSLPDRELAGVVDKIGLATDLEMPSEVPQQRTQRMSATPVVG
                     IRVRLLDPPDDLVPGLSAVVAIRRAAN"
     gene            complement(1824..2735)
                     /locus_tag="VFL84_09955"
     CDS             complement(1824..2735)
                     /locus_tag="VFL84_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608989.1"
                     /translation="MRHELKDGSPVSVKPRWSLPRGRIVLRTLRGDPQQQYLVFVPRT
                     GAADAPVLVSVHGISRNAHDQARVFATGCDERGVVLLVPIFTPDRHKDYQRLGRRGRG
                     ERTDLVLNECLAELALLTGADVAQFRMFGFSGGAQFAHRYVMAHPERVARAAFAAAGW
                     YTFPDDSQRFPYGIRPVRALRGVTFNPEKFLRVPVNVLVGERDVDSAKLRRTERTNAQ
                     QGTNRVERARNWVSAMQAASAAYGIDARVELTVVPAVDHSFDRFCREGKLMDRVFDSL
                     FPVNIAGLIGPRAVVQEVAREAPHGQL"
     gene            complement(2725..>3032)
                     /locus_tag="VFL84_09960"
     CDS             complement(2725..>3032)
                     /locus_tag="VFL84_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HET7608990.1"
                     /translation="ETADLLRSTGFARVVGGIVLAARDSMGASAGVDWMAALATPVLA
                     LSGVMTAAPLQRNEAAHATGLPIYDRDGLASPAVAIELIGRAQQHLETISRRPNDAA"
BASE COUNT          492 a         1048 c         1033 g          459 t
ORIGIN      
        1 cggtctccga gcgcgcccac ggtccgaaca gcagcgcgcc gaccatgatc gtcgcgacga
       61 ggcacaagat gatgggcagg cgcgcgtaca gcggcagccg gatccgccgc atcgacggcc
      121 gcagcgcgac gatcgcggcg acgacgacga tcatgaggcc gaagctcgcg aggaagccga
      181 tctcctgaaa gccgatcgcg atcaggatcg cgcgaagacc gagcacgcgc agcccaagcg
      241 tcaaccgggc gaacgcgacg agcaacgcgc ccgtcggcaa cgtcagcaag tagagggccg
      301 tgggccggtc gtcggccatt acccagcgaa gcgagaacga ctgattcagc agccggccga
      361 gctcgcgcag ccattcggcg cccgccggga cgtcggcgcc cggcagcgcg agcactcgcg
      421 cgagcgccat gagcagcgga atgcagccga tgacgatcaa cgcgccgagc agcagcggat
      481 cgatgtggcc cttgcggcgc acgagcccgt gccaaggcgc gcgcttcggt tcgcgcgcct
      541 gccgatcgac cgatgcgacg tggccgccca tcgccgttca gttcgccgcc ctccgaatcg
      601 ccacgaccgc cgacagcccg gggacgaggt cgtcgggcgg gtcgaggagc cgtactctga
      661 tgccgacgac gggcgtggca ctcatgcgct gcgtgcgttg ctgcggcacc tccgacggca
      721 tctcgagatc ggtcgcgagg ccgatcttgt ccacgacacc cgcgagctcg cggtcgggca
      781 gcgagcgcag cgagacagtc gctggatcgc cgacgcgcac cgacgagatg tcgtcctcgt
      841 cgatccatgc gtcgacccag acctcgcggc cgagccacat cgacatgagc ggctggccga
      901 cttcgacgga agcacccggc tgaatgattc ggcgcagaat gccgccggcc tccggcgcgc
      961 gtaccgaggc gctgtcgaga tccgcctctg ccttcgccag tcgcgctcga gcgcgctgca
     1021 cctccgcttc gaggacgccg atgccgcgtt cgcggatcgc gagcccgtcg cgcgtgaggc
     1081 gcgcgctctc ttcggcagat agtgcggcga gctgcttcgc ccgggcctcc tggaccagcg
     1141 cttgcgcggt gcgcgagccg gtctccgcat tcagaagatc ctgattcgaa attacgccgc
     1201 tggcagccaa cgattgtctc gcctcatgca gccgacgcgc gttttgcgct tggctttccg
     1261 cggccgagtt ttgtgctcgt gcggcggcga cattcgccag cgccccttgt tcttcttgct
     1321 cgacttgcaa caactcgtgc gcgatcgcgg cgcgctcgac gtcaatcttg cgttgtaggc
     1381 cttcgagctc ggcggcagcc tcgttcactt ccgcgcggaa ggttcggtcg tcgagcctga
     1441 cgaggacctg gccggccgct actcgatctc cggcgtccac ttcgacggag gcgaccaaac
     1501 cggtcaacgg cgttccgagc tcggcgatgt ggccgcgtac ggcagcgttc atcgaagtaa
     1561 cgtggaccga ctgatagtgc agccaggcga cgatcggcgc tgccagcagc gcgagcgcgc
     1621 cgaaggctcc gaagcggacc gcgtatcgtc ttgcgagcgg cagccgcgtc gttgcgagcg
     1681 ttacgagccg cagtgcgacg ccgcgtcttt gcaacgagcg gacgacgggg cggttcggct
     1741 cctctcgccg agccggccgc gactccttga ggacgacgag gctgcggtcg cggggcgacc
     1801 cgtgccgccg gcggcgaggt ttgtcagagt tggccatgcg gtgcttcgcg cgcgacttct
     1861 tgcaccactg cgcgggggcc gatgagaccc gcgatgttga ccgggaacag cgagtcgaag
     1921 acgcggtcca tgagcttgcc ttctcgacag aaccgatcga aggagtggtc gacggccggg
     1981 acgacggtca gctcgactcg cgcgtcgatt ccgtaggcgg ccgaggccgc ctgcatcgcc
     2041 gagacccaat tgcgcgcacg ctcgacacga ttcgtgccct gctgtgcgtt ggtgcgctcg
     2101 gtacgccgca gcttcgcgga atcgacgtcg cgctccccga cgagcacgtt gaccggtacc
     2161 cggaggaact tctccggatt gaacgtcacg ccgcgcagcg cgcgcacggg ccgaattccg
     2221 taaggaaagc gctggctgtc gtcgggaaac gtgtaccagc cggccgcggc gaatgccgct
     2281 cgcgcgacgc gctccggatg tgccatcacg taacggtgcg cgaactgcgc gccgccggag
     2341 aacccgaaca tgcggaactg cgcgacgtcc gcgccggtca gcagcgcgag ctccgccaga
     2401 cattcattga gcacgaggtc ggttcgctct ccgcgtcctc gccgaccgag acgttggtag
     2461 tccttgtggc ggtccggcgt gaaaatcggc accagcaaaa cgacgccgcg ctcgtcgcaa
     2521 cctgtcgcaa acacccgcgc ttggtcgtgc gcattgcgcg aaatgccgtg cacggacacg
     2581 agcaccggcg catccgccgc cccggtgcgc ggcacgaaca ccaggtactg ctgctgcgga
     2641 tcgccgcgca gtgtccgcag cacgatacgg ccgcgcggca gcgaccaacg tggcttgacg
     2701 gaaacggggc tgccgtcttt gagctcatgc cgcatcgttg ggtctccgtg aaatcgtctc
     2761 gaggtgttgc tgggcgcggc cgatgagctc gatcgctacc gccggcgagg cgaggccgtc
     2821 gcggtcgtag atcggcagtc cggtggcgtg cgccgcttca ttgcgttgca gcggtgcggc
     2881 cgtcatcacg ccgctcaagg cgagcaccgg cgtcgcaagc gccgccatcc agtcgacgcc
     2941 cgcgctcgcg cccatcgagt cgcgtgccgc gagcacgatg ccgccgacaa cgcgcgcgaa
     3001 tcccgtggag cgcagcagat cagccgtttc gc
//
LOCUS       DASSNN010000534         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_833958, whole genome shotgun sequence.
ACCESSION   DASSNN010000534 DASSNN010000000
VERSION     DASSNN010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833958"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFL84_09965"
     CDS             complement(<1..279)
                     /locus_tag="VFL84_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET7608991.1"
                     /translation="MRKALAVAAAVVVALAATGAILYARNSTPVVPPIATADAAAGPP
                     WVVKLHAQWCPVCMLTKGMWFRIETQYAGRVRLAVFDFTDEATTAASRA"
     gene            complement(283..720)
                     /locus_tag="VFL84_09970"
     CDS             complement(283..720)
                     /locus_tag="VFL84_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7608992.1"
                     /translation="MKAAAPAALAALLVIAAAPQALAHHSFAAEFDANQPVELHGKIT
                     KMEWINPHSWLHIDVKNADGTTTAWMIEGATPNTLLRRGFTREAVKAGTEITIIGFRA
                     KNGANRANGRDLILPDGSRLFMSSTGTGAPGDSEAPAEAAEPQ"
     gene            complement(717..1691)
                     /locus_tag="VFL84_09975"
     CDS             complement(717..1691)
                     /locus_tag="VFL84_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608993.1"
                     /translation="MRTRVTALSVVAVMAGGLASSVALAQTAAEPYEVPRTSWGDPDL
                     QGVWDYRTITPMERRPELGDRAYYTEEEVAQLEGRAARRMDKPPDENTPVGLVHAEYL
                     TDPGRFVDESHRTSLIVDPPNGRMPPLTPEAQARQAARGGAGGPGRAGGRADSYTDRS
                     LLERCITRGSAGAPLPTLYNNNIRITQAPGYAAIVNEMVHETRLVPLDGSPFGNVRSY
                     NGESRGHWEGDTLVVETRNFNGKAPYRGAGVNLKLTERYTRVGPDKIDFKVTFEDATQ
                     WTQPWTAEYSMRAGEELYEYACHEGNYGLRNILENARDEERKAAGARR"
     gene            1954..>3078
                     /locus_tag="VFL84_09980"
     CDS             1954..>3078
                     /locus_tag="VFL84_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET7608994.1"
                     /translation="MLLALGGAADAALAEGSAASGIDEIEVIGQRPTYRISETSTATK
                     TPTRLIDIPQSLTLIGSGMIRDQAMQNMGDVVRYVPGVQMAQGEGHRDAPIFRGNTST
                     ADFFVDGMRDDTQYYRDLYNVEQVEVLKGPSGMIFGRGGSGGLINRVTKQADGMNLQE
                     FGATLGSWEDRRLTADVGRAIGDGSAFRVNAFYEDSESYRNFGKLNRWAINPTFTLER
                     SDATRVTLGYERFEDERVTDRGIPSQLPLLGLPLETGASTFFGSPDSSPTSATVDAFA
                     STVTHDFSDHLTLTNKTRLADYDKFYQNVFPGAYSAADGRVSVSGYSNRTARENLLNQ
                     TDVTFSAETAGVSHTVLSGMELGRQHTDNFRQTAYFTAVGS"
BASE COUNT          494 a         1004 c         1013 g          567 t
ORIGIN      
        1 ggcgcggctc gcggccgtcg tcgcttcatc cgtgaagtcg aacaccgcga gccgcacgcg
       61 gcccgcatac tgcgtctcga tccgaaacca catgcccttc gtcagcatgc agacggggca
      121 ccattgcgcg tgcagcttca cgacccacgg cggtccggcc gcggcgtcgg ccgtcgcgat
      181 cggcggcacg accggcgtcg aattgcgcgc gtacaggatc gcacccgtcg cggccagagc
      241 gaccacgacg gcggcggcga cagcgagagc cttgcgcatg cgctattgcg gctcggctgc
      301 ctcggccggc gcttcgctgt cgcccggcgc gcccgttccc gtcgacgaca tgaacaagcg
      361 cgagccgtcg ggcaggatca agtcgcggcc gttggcgcgg ttcgcgccgt tcttcgcgcg
      421 aaagccgatg atcgtgatct cggtgccggc cttgacggcc tcgcgcgtga agccgcggcg
      481 cagcagcgtg ttcggcgtcg cgccttcgat catccatgcc gtcgtcgtgc catcggcgtt
      541 cttcacgtcg atgtgcagcc acgagtgggg attgatccac tccattttcg tgatcttgcc
      601 gtgcagctcg acgggctggt tcgcgtcgaa ctcggccgcg aacgagtgat gtgcgagcgc
      661 ttgcggagcc gcggcgatga cgagcagggc ggcgagcgcc gccggtgcgg ctgccttcat
      721 cggcgcgccc ccgcggcttt gcgctcctcg tcgcgtgcgt tctccaagat gttgcgcaag
      781 ccgtagttgc cctcgtgaca ggcgtactcg tagagctctt cgccggcgcg catcgagtat
      841 tccgcggtcc agggctgcgt ccactgcgtg gcatcctcga acgtgacctt gaagtcgatc
      901 ttgtcgggac cgacgcgcgt gtagcgctcc gtcaatttca gattgacccc ggcgccgcga
      961 tacggcgctt tgccgttgaa gttgcgcgtt tccacgacga gcgtgtcgcc ttcccaatgg
     1021 ccgcgcgatt cgccgttgta gcttcgcacg ttgccgaacg ggctgccgtc gagcggcacg
     1081 aggcgagtct cgtggaccat ctcgttcacg atcgcggcat agcccggcgc ctgcgtgatg
     1141 cggatgttgt tgttgtagag cgtcggcagc ggcgcgccgg cggagccgcg cgtgatgcag
     1201 cgctcgagca acgagcggtc ggtgtacgaa tcggcgcggc cgccggcgcg gcccggaccg
     1261 cctgcgccgc cgcgcgcggc ttgccgcgct tgcgcttcgg gcgtcaacgg cggcatgcgg
     1321 ccgttcggcg ggtcgacgat cagcgaggtc ctgtgcgact cgtcgacgaa gcggccggga
     1381 tcggtcaggt actcggcgtg cacgagaccg accggcgtgt tctcgtcggg cggcttgtcc
     1441 atgcggcgcg cggctcgccc ttcgagctgc gcaacttctt cttcggtgta gtacgcgcga
     1501 tcgccgagct cgggcctgcg ctccatcggc gtgatcgtgc ggtaatccca gacaccctga
     1561 aggtcgggat cgccccagga cgtgcgcgga acctcgtagg gctccgcggc cgtttgcgcc
     1621 aaggcgacgg acgaggcgag accccccgcc atgaccgcca ccaccgagag cgccgtgact
     1681 cgcgttctca ttgctccccc gaaaaaggtt gtaacgccgc taccgctctc gatcgtagca
     1741 ccggagtcgt accacgcgac gacggcgtga gcggccatgt tttgttgaga atgagttgca
     1801 ttggccgtac ggtcatgatg ataatgcgaa ctactcgcat ttgcattgag aagggagcgc
     1861 gacgtgagca tcaacggttt cgggggaatt cagggggcgt ttaacaacac caagcgcgcg
     1921 gcgtcgttgg ctcgacgacc actttgcgtc gcgatgctcc ttgccctcgg cggcgctgcc
     1981 gacgccgctc tggccgaggg ctccgctgct tcaggcatcg atgagatcga agtgatcggc
     2041 cagcgcccaa cttatcgcat ctccgagacc tcgacggcga cgaaaacccc gacgcggctg
     2101 atcgacattc cgcagtccct cacgctgatc ggcagcggca tgatccgcga ccaagccatg
     2161 cagaacatgg gcgacgtcgt gcgttacgtg ccgggcgtgc aaatggccca aggagagggc
     2221 caccgcgacg cgccgatctt tcgtggcaac acttcgaccg ccgatttctt cgtcgacggc
     2281 atgcgcgacg atacgcagta ctaccgcgat ctctacaacg tcgagcaagt cgaggttttg
     2341 aagggaccga gcggcatgat tttcggccgt ggtggctcgg gtggattgat caaccgtgtc
     2401 acgaagcagg ccgacggcat gaatctacaa gagttcggcg cgactctcgg ctcttgggaa
     2461 gatcgccgcc tcacggccga tgtcggtcga gccatcggcg acgggtcggc gtttcgagtc
     2521 aacgcgtttt acgaggactc ggaaagttat cgcaacttcg gcaagctcaa ccgctgggcg
     2581 atcaacccga cgttcacgct ggagcgaagc gatgcgacgc gcgtgacgct cggttacgag
     2641 cggttcgagg acgaacgcgt gaccgatcgc gggataccct ctcagttgcc tttgcttggg
     2701 ctcccgctcg agaccggtgc gtcgacgttc ttcggctcgc ccgactcgag ccccacgtcc
     2761 gcgacggttg acgccttcgc gtcgactgtg acgcacgact tttcggacca tctgacgctg
     2821 acgaacaaga cgcggttggc cgactacgac aagttctatc agaacgtatt tccgggcgcg
     2881 tattcggctg cggacggccg cgtcagcgtt tcaggctaca gcaatcgcac ggcgcgcgag
     2941 aatcttctga accagaccga cgtgacgttc tccgccgaga cggccggcgt ttcgcacacc
     3001 gtgctctcgg gcatggagct tggacggcag cacaccgaca acttcaggca aacggcctat
     3061 ttcaccgcgg ttggctcc
//
LOCUS       DASSNN010000535         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_327008, whole genome shotgun sequence.
ACCESSION   DASSNN010000535 DASSNN010000000
VERSION     DASSNN010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327008"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VFL84_09985"
     CDS             <1..443
                     /locus_tag="VFL84_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase F"
                     /protein_id="HET7608995.1"
                     /translation="MMMLTEHTANMMEVNNRHDARSSVLGGGHYYLRVRRKVPERIGE
                     PDRTWLAVAAYNLGFGHLEDARILTQFQGADPDQWEEVRERLPLLSDKAWFSRVQRGF
                     AAGQTAVTYVDNVRRYYEILMWLDSHETLTSRDFAPPNALGPAG"
     gene            complement(440..922)
                     /gene="tadA"
                     /locus_tag="VFL84_09990"
     CDS             complement(440..922)
                     /gene="tadA"
                     /locus_tag="VFL84_09990"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004552523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HET7608996.1"
                     /translation="MNSSGVDPDRALMEQALACAREAVAAGEVPVGAVIADATGAVVA
                     RAANAPIARSDASAHAEILALRAAGRAAGNYRLPGCVLYVTLEPCAMCVGALVHARVA
                     RIVYGAPDPKTGACGSVFDLASSPKMNHRIEVVGGVLAEQCGALLKQFFAARRGRDTL
                     "
     gene            1040..1693
                     /locus_tag="VFL84_09995"
     CDS             1040..1693
                     /locus_tag="VFL84_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7608997.1"
                     /translation="MIYIEFMSRRRQPGFHANNLGALVIEPFESQLERFHRGVLAGQS
                     GWEGSWSEDRMLLSAGRTWRLGPEPEYMTAWQTRTGFARIDEWDKVFRSGQADELEKR
                     FREVARIDRAGCYEALLEPSRAHGGIYYAEFFEPAGSNDEIGAFYERRAAAHAELTLA
                     LCLARIGKLAPDPGGLAVWTLPSFAALAAIASELDGTTEPVLLTTAGVYNDIGKEIL"
     gene            1694..2491
                     /locus_tag="VFL84_10000"
     CDS             1694..2491
                     /locus_tag="VFL84_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET7608998.1"
                     /translation="MDTEEKYLFDLMGFLVLPQILTREEVAELNALIDSYDLWRKAKS
                     GEEPAWVNDPNFMTVGAPHTWDEPFRRLLAHPKIMPYLKTLVGPKFRYDHGHALLMRA
                     GAKQLGLHGGATPWDPAQEYRYRDGEMLNGLVVVSYALSDASADDGGFAVVPGSHKSN
                     FAVPPQFIKFERVGPWVLRVPVKAGDAIIFSEACTHGTWPWRAKHERRSLLYKFAPGH
                     SAWASPYPSPADAPELDYPEEQKRILDQPYVAPDQDRFGHEYRKNVQ"
     gene            complement(2501..>3241)
                     /locus_tag="VFL84_10005"
     CDS             complement(2501..>3241)
                     /locus_tag="VFL84_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HET7608999.1"
                     /translation="PELPGVQRALAEAVLERARANGVPVVVVLFSGRPLIVPWLFERA
                     DALLLAWFPGTEAGNAIADVLTGRVSPSGRTPITWPRALGQIPLFYGQRNSGRPENPH
                     DHFTSKYLDCPSSPLIPFGFGLTYGRFTYANLAVSPETAAAEDVIEVTVDVTNEGAHA
                     AEETVFLFIRDRVASVARPTLELKGVAKLSLRPGETGTARFELPATELAFLGVDFEPV
                     LEPGEIEIHVGGSADPAELASSRVMLRP"
BASE COUNT          549 a         1116 c         1092 g          484 t
ORIGIN      
        1 gcatgatgat gttgaccgag cacaccgcca acatgatgga ggtcaacaat cgccacgatg
       61 cgcgctcgag tgtcctcggc ggcggccact actatctgcg cgtgcgccgc aaagtgcccg
      121 agcgcatcgg cgagccggac cgcacgtggc tcgcggtcgc ggcgtacaac ttgggcttcg
      181 gccatctcga ggacgcgcgc atcctgacgc agttccaagg cgcggatccg gatcagtggg
      241 aagaggtgcg cgagcgcttg ccgctcttga gcgacaaggc ttggttcagc cgcgtgcagc
      301 gcggcttcgc cgcaggccag accgcggtca cgtacgtcga caacgtgcgc cgctactacg
      361 agatcctgat gtggctcgac agccacgaga cgctgacgtc gcgcgacttc gcgccgccca
      421 acgccctcgg tcccgccggt tagagcgtgt cgcggccgcg ccgcgccgca aagaactgct
      481 tcagcagcgc gccacactgc tccgcgagca cgccgccgac gacctcgatt cggtgattca
      541 ttttcgggct cgaggcgagg tcgaacaccg agccgcacgc tccggtcttc ggatcgggcg
      601 cgccatagac gatgcgcgcg acgcgcgcgt gcacgagcgc gccgacgcac atcgcgcacg
      661 gctcgagcgt cacgtacaga acgcatcccg gcagacgata gttcccggcc gcgcggcccg
      721 ccgcgcgcag cgcgagtatc tccgcgtgcg cggacgcgtc gctgcgcgcg atcggcgcgt
      781 tcgcggcgcg cgccacgacg gcgcccgtcg cgtcggcgat cacggcgccg acgggcacct
      841 cgcctgccgc aacggcctcg cgcgcgcacg cgagcgcctg ctccatcagc gcgcgatccg
      901 gatccacacc gctcgaattc atgccggtca tcatcacgca gcaatccgcg ttcgcaccag
      961 cccgagcgcg gctcggcgtg gcgtgccgcc cggcccggaa cttgcttttc ctcggtcggt
     1021 catctcttcg agcgagacca tgatttacat cgaatttatg agccggcgcc gccagccggg
     1081 cttccacgcg aacaacctgg gcgcgctggt catcgagccg ttcgagagcc agctcgagcg
     1141 cttccatcgc ggcgttctcg ccggacaaag cggctgggaa ggctcgtggt cggaggaccg
     1201 catgcttctc agcgccggcc gcacctggcg gctcggaccc gagccggagt acatgacggc
     1261 ctggcaaacg cggaccggct tcgcgcgaat cgatgaatgg gacaaagtgt ttcgctctgg
     1321 ccaagcagac gagctcgaga agcggttccg cgaggtcgcg cgcatcgacc gcgcgggctg
     1381 ctacgaggcg ctgctcgagc cgagccgcgc gcacggcggc atttactatg cggaattctt
     1441 cgagcccgcg ggctcgaacg acgagatcgg cgcgttctac gaaagacgtg ccgccgcgca
     1501 tgccgagctg acgctcgcgt tgtgcctcgc caggatcggc aagctcgctc cggaccccgg
     1561 aggcctcgcg gtttggacgc tgccgtcgtt cgcggcgctc gccgcgatcg caagcgagct
     1621 cgacggcact accgagcccg tgcttctgac gaccgcgggc gtctacaacg acatcggaaa
     1681 ggagatcctg taaatggaca ccgaagaaaa atacttgttc gacctgatgg gcttcctcgt
     1741 cctgccgcag atactgacgc gcgaggaggt cgcggagctc aatgcgctga tcgactccta
     1801 cgacctatgg cgcaaagcga agtccggcga ggagccggcg tgggtcaacg atccgaactt
     1861 catgaccgtc ggcgcgccgc acacctggga cgagccgttc aggcgcctgc tcgcacatcc
     1921 gaagatcatg ccgtacttga agacgctggt cggtccaaaa ttccgctacg accacgggca
     1981 cgcgctgctg atgcgcgccg gcgcgaagca gctcggcctc catggcggcg cgacgccgtg
     2041 ggatccggcg caggagtacc gctaccgcga cggcgagatg ctgaacgggc tcgtcgtcgt
     2101 gtcgtacgcg ctcagcgacg cgagtgccga cgacggcggc ttcgccgtcg tgcccggcag
     2161 ccacaaatcg aacttcgcgg tgccgccgca gttcatcaag ttcgagaggg tcgggccgtg
     2221 ggtgcttcgc gtgccggtca aagccggcga cgcgatcatc ttcagcgagg cgtgcacgca
     2281 cggcacttgg ccgtggcgcg cgaagcacga gcgccgcagc ctgctctaca agttcgcgcc
     2341 ggggcactcc gcgtgggcgt caccgtatcc gtcgccggcc gacgcgcccg agctcgatta
     2401 tcccgaggag cagaagcgta tcctcgatca gccctatgtc gcgccggacc aggacaggtt
     2461 cgggcatgag taccgaaaga acgtccagtg aacgcgcgat ctagggccgc agcatcacgc
     2521 gcgaggacgc gagctcggcg gggtcggcgc ttccgcctac gtgaatctcg atttcgccgg
     2581 gctcgagcac cggctcgaag tcgacgccca agaacgcgag ctccgtggcc ggcagctcga
     2641 accgcgcggt gccggtctcg ccgggccgca acgaaagctt cgcgacgcct ttcagctcga
     2701 gcgtcgggcg cgcgacgctc gcgacgcgat cgcggatgaa caagaacacc gtttcctcgg
     2761 ccgcatgagc gccctcgttc gtcacgtcga cggtgacctc gatgacgtct tcggccgcgg
     2821 cggtctcggg gctcacggca agattcgcgt acgtgaaacg gccgtacgtg agaccgaagc
     2881 cgaacggaat cagcggcgag ctcgggcagt cgaggtactt gctcgtgaag tggtcgtgcg
     2941 ggttttcggg ccggcccgag ttgcgctggc cgtagaacag cggaatctgc ccgagcgcgc
     3001 gcggccacgt gatcggcgtg cgtcccgacg gcgacacgcg gcccgtcagc acgtccgcga
     3061 tcgcattgcc cgcttcggtg ccggggaacc acgcgagcag cagcgcatcg gcacgctcga
     3121 agagccatgg gacgatcaac ggccggccgc tgaagagcac gacgacgacg ggcactccat
     3181 tggcgcgggc gcgctcgagc acggcctccg cgagcgcgcg ttgcaccccg ggcagctcgg
     3241 g
//
LOCUS       DASSNN010000536         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_834214, whole genome shotgun sequence.
ACCESSION   DASSNN010000536 DASSNN010000000
VERSION     DASSNN010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834214"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VFL84_10010"
     CDS             complement(<1..583)
                     /locus_tag="VFL84_10010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7609000.1"
                     /translation="MAQRNELDVNQFLDTRRVGATHILVLSLLILTMMVDGYDIFVVG
                     TVAPYLAADLAIPPDSLTSVLVITQFGLMLGNFFVGPVADRYGRRFTMLWCLFAFGLL
                     TLATPLATTVGELFVLRFVAGLFLSGVIPNAIALVSEIMPSRVRATFVSITFAGYTLG
                     VSVIGAPVVKWLVPLGWEYAFVVGGALPLVLAAV"
     gene            complement(587..1963)
                     /locus_tag="VFL84_10015"
     CDS             complement(587..1963)
                     /locus_tag="VFL84_10015"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET7609001.1"
                     /translation="MSKAAHSKDGAQGSTLGELARILFDIKFAALDAATLDAVRARVL
                     DALGALVVGLQTPEGRVLRRYAQRASGRGGTLDFAERCRLYVGAARATEVDDIDVASC
                     TTVGSVVVPVALGVAARRSLADPRTLLAAVVAGYEAMQRLGRAIGGATLIYRGVWPTY
                     VTAPFAAAATAAKLLELDAAATARALALALTRTSSVPAAALSRSGFRYYSLGCAAVEG
                     ADAASAAAAGVEADQDALAAFAERIGAGLDAALVHAAGEPWLVREVDSKPWPTSRQAL
                     ASVAAFRALELAPASIDAIERIVVGVPGAYRAMIDRAAPPAQRIESMIGVQYQVALAA
                     LTPDKLYDAIRTEVPAPPELASLMAKIEVRADEALDARFPRRWGSRVTVRFGSGEERT
                     HEVLDPDGAAARPLDLDGVERKYARIFAASNVDKADWLGAARERCEQLARAAGDVGLV
                     ADLWASIE"
     gene            complement(1976..>2912)
                     /locus_tag="VFL84_10020"
     CDS             complement(1976..>2912)
                     /locus_tag="VFL84_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET7609002.1"
                     /translation="LQSGGALLVPLEGAPVYVGFGGGRELSFVRTLTWLDQVMSGGGP
                     KAFEVVRDQLRARGIASGPIAIAGAAGLAAGAEARVQKAFADYTLRPFDAELRALRAV
                     KRPREILAMRLTLGVVEDAVYAGLAKFTGGGDNAAAMLEAERVARARKARDVRVLVNM
                     GGPELRPFEGRLDGRHAPVRIWVAAQYQGYWAEAAAMAPVSQPTAAQKAVAAMLGAAR
                     EGATTGEVAAAAVAALSPAEGQAALEYGLGNTIGLSHDEGVRIRLGGSERLVAGSVLA
                     LRAHAAAGPNPSIDAAVASVGPSGAQRLQSLRIAG"
BASE COUNT          456 a         1079 c         1012 g          365 t
ORIGIN      
        1 gcaccgcggc gagcacgagc ggcagcgccc cgccgacgac gaaggcgtat tcccacccca
       61 acggcacgag ccacttcacg accggcgcgc cgatcacgga cacgccgagc gtgtagcccg
      121 cgaacgtgat cgagacgaac gtcgcgcgca cgcgcgacgg catgatctcc gagacgagcg
      181 cgatcgcgtt cggaatcacg ccgctcaaga agaggcccgc gacgaagcgc agcacgaaca
      241 gctcgccgac cgtcgtcgcg agcggcgtcg cgagcgtcag caggccgaac gcgaagagac
      301 accagagcat cgtgaagcgg cgcccgtagc gatcggccac ggggccgacg aagaaattgc
      361 cgagcatcaa gccgaattgc gtgatgacga gcacgctcgt cagcgaatcg ggtgggatcg
      421 cgagatcggc cgccagatac ggcgcgacgg tgccgacgac gaagatgtcg tagccgtcga
      481 ccatcatcgt caggatcaac agcgacaaga cgaggatatg cgtcgcgccg acgcggcgcg
      541 tgtcgaggaa ctggttgacg tcgagctcgt tgcgctgcgc catgtcttac tcgatcgacg
      601 cccacaggtc tgcgaccaga ccgacgtcgc ccgccgcgcg ggcgagttgc tcgcagcgtt
      661 cgcgcgcggc gccgagccaa tctgccttgt cgacgttgct cgcggcgaag atgcgcgcgt
      721 acttgcgctc gacgccgtcg agatcgagcg gccgcgccgc tgccccatcg ggatcgagga
      781 cctcgtgcgt gcgctcctcg ccggagccga aacgaaccgt gacgcggctg ccccaccggc
      841 gcgggaaacg cgcgtcgagc gcctcgtcgg cgcggacctc gatcttcgcc atcaacgacg
      901 cgagctccgg cggcgccggc acttcggtgc gaatggcgtc gtagagcttg tccggcgtca
      961 acgccgcgag cgccacctgg tattgcacgc cgatcatcga ctcgatgcgc tgcgcgggcg
     1021 gcgcggcgcg gtcgatcatt gcgcgatacg cgccgggcac tccgacgacg attcgctcga
     1081 ttgcgtcgat gctcgccggc gcgagctcga gcgcgcgaaa cgcggcgacg ctcgcgagcg
     1141 cttgccgcga cgtgggccac ggcttgctgt ccacttcgcg cacgagccag ggctcgccgg
     1201 ccgcgtggac gagcgccgca tcgagccccg cgccgattcg ctcggcgaat gccgcgagcg
     1261 catcttggtc cgcctcgacg cccgcggccg cggcgctcgc ggcgtccgcg ccttcgacgg
     1321 ctgcacaacc gagcgagtag tagcgaaaac cggagcgcga caatgccgcg gcaggcacgc
     1381 tcgacgtgcg agtcaacgcg agcgcgagcg cacgcgcggt cgcggccgca tcgagctcga
     1441 gcagcttcgc ggcggtcgcg gctgccgcga atggcgccgt gacgtacgtc ggccagacgc
     1501 ctcgatagat caacgtcgcg ccgccgatcg ctcggccgag gcgctgcatc gcctcgtagc
     1561 ccgccacgac ggccgcgagc agcgtccgtg gatcggcgag cgagcgtcgg gccgcgacgc
     1621 ccagcgcaac gggcacgacg acggagccga ccgtcgtgca ggatgcgacg tcgatgtcgt
     1681 cgacctccgt cgcgcgcgcc gcgccgacgt agagccggca acgctcggcg aagtcgagcg
     1741 tgccgccgcg cccgctcgcg cgctgcgcgt agcggcgcag cacgcgtcct tcgggcgttt
     1801 gcaagccgac gacgagtgcg ccgagcgcgt cgagcacgcg tgcgcgcacg gcatcgagcg
     1861 tcgccgcgtc gagtgccgcg aacttgatgt cgaacagaat tcgcgcgagc tcgccgagag
     1921 tcgaaccctg ggcgccgtcc ttcgagtgcg cggccttgga cattcgccgc cggggctagc
     1981 ccgcgatccg caacgactgc aagcgctgcg cgccggaggg tccgacgctc gcgaccgcgg
     2041 cgtcgatcga agggttcgga ccggccgcgg cgtgcgcgcg cagcgcgagc acgctgcccg
     2101 cgacgaggcg ctcgctgccg ccgaggcgaa tccgcacgcc ttcgtcgtgc gaaaggccga
     2161 tcgtgttacc gagcccgtac tcgagcgcgg cctgcccttc ggccggcgac agcgccgcga
     2221 cggcggccgc cgcaacctcg cccgtcgtcg cgccctcgcg cgctgcgccg agcatcgcgg
     2281 cgaccgcttt ctgcgccgcg gtcggctgcg agaccggcgc catcgcggcc gcctcggccc
     2341 aatagccttg gtactgcgcc gcgacccaga tccggaccgg cgcatgccgg ccgtcgagcc
     2401 gcccttcgaa cggccgcagc tcgggcccgc ccatgttgac gagcacgcgc acgtcgcgcg
     2461 ccttgcgcgc ccgcgcgacg cgctccgctt cgagcatcgc ggccgcgttg tcgccgccgc
     2521 ccgtgaattt cgcgagcccg gcatagaccg cgtcttcgac gacgccgagc gtcaagcgca
     2581 tggccaaaat ttcgcgcggc cgtttcacgg cacgcaacgc gcgcagctcc gcatcgaacg
     2641 gccgcagcgt gtagtcggcg aacgcttttt gcacgcgcgc ctccgcaccg gccgcgaggc
     2701 ccgcggcgcc cgcgatcgcg atcggcccgc tcgcaatccc gcgcgcgcgc aattggtccc
     2761 gcacgacctc gaatgccttc ggcccgccgc cggacatcac ctggtcgagc cacgtcagcg
     2821 tgcgcacgaa agacagctcg cggccgccgc cgaacccgac gtagacgggt gcaccttcga
     2881 gcgggacgag cagcgcgccg ccggactgaa gg
//
LOCUS       DASSNN010000537         9690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1105911, whole genome shotgun sequence.
ACCESSION   DASSNN010000537 DASSNN010000000
VERSION     DASSNN010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105911"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2565)
                     /locus_tag="VFL84_10025"
     CDS             complement(<1..2565)
                     /locus_tag="VFL84_10025"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609003.1"
                     /translation="MSYDTRNVFQACSRHSRVLVLALIAAAVTACSGDGDGSVGVGTG
                     QDPDPVAPDFPIAYTKGPLNDEDDQLQVNADLRDILRFAVGTDLYIRDRASPTAPERN
                     VTMRFTNGTGDVSGVEISSDGRKVLFAMRGPVDEGLALDDPDQPKWNIWEYEIATDNL
                     RRLIASDLTAQQGHDLSPHYLADGRIIFVSTRQQTAKAILINDGKPQYDARDEDRDNP
                     AFVLHVMRDDGRDEFKQVSFNQSSDYEPTVLDNGKVLFSRWDHAGNVNGIHLYQMNPD
                     GTELELLYGAESHLTGTNNEAVQFVSAREMFDGRIMAIVRPFDHPELGGAITIIDAKT
                     YVENTQPIAAFTGMPGPAQTAATPNQVRTDLVPSPGGRFSSAFPLWDGTGRVLTTWSI
                     CRLEEPDPANPTDPTAVIYRPCTPERLAATNPAPVVAPPLYGVWMYDPQTQTQQPIVI
                     GEEGVIISDIVAAQPRRAPAYIPDKLPGLDFDADLAAEEAGIINIRSVYDLDGTASVD
                     IAAVANPVITPPANRPARFLRIEKAVAIPDEDTLDLDDTAFGPNIQQGMREVIGYAPI
                     EPDGSVRVKVPANVALALSVLDGNSRRISARHQNWIQVVPGQELTCNGCHAPASGLSH
                     GRSTAFNSAYAGAPSTGVPFPGSVGTFSPDVGETMAETRTRVSCQTDCAALEPSVDVL
                     YTDVWTDPAVATPAAPVSYLYSNLTTAVPTSINCIQNWTPRCRTIINYETHIHPLWNT
                     PRPVLDAMGNVIGSNTCSQSGCHAPVNAMNAAMVPAGQLDLSDGVSPDEADHFNSYQE
                     LLFGDNRQILVGGAIQDEQQVVGTDAMGNPILDFVPVVPPMSPAGARQSRFFSCFDV"
     gene            3067..4275
                     /locus_tag="VFL84_10030"
     CDS             3067..4275
                     /locus_tag="VFL84_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609004.1"
                     /translation="MSNRMHVAPLAILGNHALLWRIFVALALLLCVLCGASIALAGPR
                     EQAARMHDRLAGVPADEATLNAMAATLPGNPTAAANIAMSNPNFYAVTLKNFSAPWTN
                     RDQNAFVPLNDYMALVMGMVRDDVPFNQILTANLLYTGPASVTPAPSPTSNAHFEALE
                     ARMRDSTFDQSQLVQTTQTAVYGTPAAATAGAITTRAAAEAFFIAGTNRAMFRFTLMN
                     HMCMDMEQVHDTSIAPDRIRQDVSRSPGGDSRVFLNNCIGCHAGMDPLAQAFAYYDFD
                     ETSQRLVYDSSAVNSKYFNNDATFPDGFVTPDDSWHNHWRQGQNALLGWNAALPGQGN
                     GAKSLGEELAGTQAFAQCQVKKVFKAVCLRDPVDANDRNQIDAMTNTFRNAYNLKQVF
                     AESAGYCMGN"
     gene            4299..6914
                     /locus_tag="VFL84_10035"
     CDS             4299..6914
                     /locus_tag="VFL84_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007619686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HET7609005.1"
                     /translation="MQYASLMSVESPGVGATKRGRSLPTRLAGGGAVLVAAMLAACSG
                     GSGADVEETPGQPVDVTSSNYNGPPPSTADVQSFKINLWDNVQANNRCGTCHSDTGGQ
                     APMFARRDDINLAYAAANGVVTLASPQDSQMVAKVGGGHNCWLASDAACGDILTTWIT
                     NWAGSTASTAARKIELEPPVLRDPGQSKNFPPDQGALFSTTVYPILEQHCSECHASNT
                     ALKQQPFFAEGPPSDSDAVELAYEAAKPKMNLDDPAISRLVVRLRNESHNCWTSSCTN
                     DANVMETAIQNFSNNVTPTSVDPSLVTSKATTLYEGTIASGGNRYEANVIAMYEFKTG
                     QDCGTSITGVCSLAYDTSGVDPAMDLTLSGNVQWFGGWGLNFAGGKAQASTSSSAKLK
                     NLILSTGEYSIEAWVAPGNVVQEDMRIVSYSASLTNRNFNLGQTMYDYDFFTRSSVSS
                     ANGDPALSTPSADEVLQATLQHVVATFHPATGRRIYVNGVLVATDAAPGGTITNWDTS
                     YAFVLGNEVSGNRMWNGVIRLVAIHNRALTQAQIQQNFDAGVGEKFFLMFGVEHLTNI
                     NDSFIVVEAAQFDSYGYLFRTPFFISLDGTAQPAGLNIRGMRVGLNGAEAHVGQAFAN
                     LDTQISGTVYTPGTGQTLLNLGTVLPLEKGPDEDEFFLTFDTLGANTYSRPPPAVPPA
                     PTPEDLPPASAIGVRTFDEISASIATLTGVSQNDPGVRAAVDEVRQSLPAVPSLEAYL
                     SSHQAAVGQLAVEYCHALIEDPALRAATFPGFNFTTTPAAAFANENALFDPLLDRTLG
                     LVQLGHQPDKNAVRTELSRLVNGYPDDAGLSGVVRPGLLNALPPGQANDEQRTRAIAK
                     AVCASVVGSAAMLIQ"
     gene            6943..8607
                     /locus_tag="VFL84_10040"
     CDS             6943..8607
                     /locus_tag="VFL84_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609006.1"
                     /translation="MLIRVKRKLRHPEAPQRHENHRRPMTRRELIAQGFMTGGAAVLT
                     TGLFSLFANPRAALAQLAEDLAADAGVLGCGVDRTGTKIPFICFDLAGGANLAGSNVL
                     VGRGGALGQLDTLSTAGYSKLGLPGDQVPGVAEAAPSATGTSNGLHADTSLGLAFHSD
                     SAFLRGMYASFKGDAAAIAAGTNGAVIPARSENDTGNNPHNPLYGIQRAGAEGSILTL
                     IGSQNTDSGGNSMAPAMMINAEFRPTKVDRPSDVTGLVGTSDRPKRLSHQDEVAVTEA
                     IYRLSKRRIDNNVSTGLIGPDPVRAPITRDQVVEELVKCGYLDAAYTKEKFGNVVLNP
                     AADLDIVGPTGIFTDAEFNAGGTDGNEFRKTASVMKMVIDGYAGAGCITMGGYDYHGG
                     MRQEGETKDFRAGRCMGAVLAYAMRRGKPVMMYVFSDGSLSSNGVIDNSVAGRGKGEW
                     VSDNQDMAAPFFLVYNPTGQRPTLLTSQNMTAAQHQQLGAFSADGNVVRSGTPGANNV
                     NLLVHMILLNYMALHGEQGQFTTLFPNHGLGDPALLDRFIAFAPIV"
     gene            8791..>9690
                     /locus_tag="VFL84_10045"
     CDS             8791..>9690
                     /locus_tag="VFL84_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609007.1"
                     /translation="MTAAEALRELLACPRCDAPLTEAGEAWRCGGCEVEFPRLAGIPW
                     LFAEPNAALAEWRVRLHFALQKLERDRQNIAASLAGAPLRTATRARLESLERATREHG
                     ERLRALLAPLELDRHAASYEVHLALRTRLPAHQGLTTYYANIHRDWCWGEEENAASYE
                     TLAAWLRAAPPSRALVLGAGAGRLAYDLHVQTAARTTVALDFNPLLSIVADNVTHGRA
                     IELYEFPIAPRGDAALLRTLSAPSPARAGLAFVLADAHRPPLRRGAFDTVVTPWLVDI
                     LPERFDILCARVNALLGDGGHWLN"
BASE COUNT         1657 a         3270 c         3197 g         1566 t
ORIGIN      
        1 gacgtcgaag cacgagaaga accgcgactg ccgcgcgccg gccgggctca tgggaggaac
       61 gaccggcacg aagtccaaga tcggattgcc catcgcgtcc gtgccgacga cctgctgctc
      121 gtcctggatt gcgccgccga cgaggatctg gcggttgtcg ccgaacaaca gctcctgata
      181 cgagttgaaa tgatccgcct cgtccggcga aacgccgtcg ctcaagtcga gctggccggc
      241 cggcaccatc gccgcgttca tcgcgttgac gggcgcgtgg cagcccgatt gcgagcacgt
      301 attgctgccg atcacgttgc ccatcgcgtc aagcaccggc cgcggcgtat tccacagcgg
      361 atggatgtgc gtctcgtagt tgatgatcgt gcggcaccgc ggcgtccaat tctggatgca
      421 gttgatgctc gtcggcaccg ccgtcgtcag gttcgagtac aggtacgaca cgggcgcggc
      481 cggtgtcgcg actgccggat ccgtccacac gtccgtatag agaacgtcga cgctcggctc
      541 gagggccgcg caatcggtct ggcagctgac gcgcgtgcgc gtctccgcca tcgtctcgcc
      601 gacgtcgggc gagaacgtgc cgacggagcc tgggaacggg acgcccgtgc tcggcgcgcc
      661 ggcgtaagcc gagttgaacg ccgtgctgcg gccgtgcgag aggccgctcg ccggagcgtg
      721 gcaaccgttg caggtcagct cttgccccgg cacgacctga atccagttct gatggcgcgc
      781 ggagatgcgc cggctattgc cgtcgagcac gctcagcgcg agcgcgacgt tggcgggcac
      841 tttgacgcgc acggagccgt cgggctcgat cggcgcgtag ccgatgacct cgcgcatgcc
      901 ctgctgaatg ttcgggccga acgccgtgtc gtcgaggtcg agcgtgtcct cgtccgggat
      961 cgcgaccgcc ttctcgatgc gcagaaagcg cgcaggcctg ttcgccggcg gcgtgatgac
     1021 gggattcgcg acggccgcga tgtcgacgct cgccgtgccg tcgagatcgt agacgctgcg
     1081 aatattgatg atgccggcct cctcggccgc gaggtcggcg tcgaagtcga ggcccggcaa
     1141 cttatcgggg atgtaggccg gggcgcggcg cggctgcgcg gcgacgatgt ccgagatgat
     1201 gacgccttcc tcgccgatca cgatcggctg ctgcgtctgc gtttgcgggt cgtacatcca
     1261 cacgccgtac agcggcggcg cgacgacggg cgcagggttc gtcgcggcga ggcgttccgg
     1321 cgtgcacggc cgatagatga cggcggtcgg gtcggtcgga ttggccgggt cgggctcctc
     1381 gagccggcag atcgaccagg tggtcaagac gcggcccgtg ccgtcccaga gcgggaacgc
     1441 cgagctgaag cggccgcccg gcgagggcac gagatccgtg cggacttgat tgggcgtcgc
     1501 ggccgtttgc gccggaccgg gcatgcccgt gaacgccgcg atcggctgcg tgttctcgac
     1561 gtaggtcttc gcgtcgatga tcgtgatcgc gccgccgagc tcgggatgat cgaacggccg
     1621 gacgatcgcc atgatgcggc cgtcgaacat ctcgcgcgcg ctgacgaact gcaccgcctc
     1681 gttgttcgtg ccggtcaggt ggctctcggc gccgtacagc agctcgagct ccgtgccgtc
     1741 gggattcatt tgatataggt gaatgccgtt cacgttaccg gcgtgatccc agcggctgaa
     1801 cagcaccttg ccgttgtcga ggacggtggg ctcgtagtcg ctgctctggt tgaacgacac
     1861 ctgcttgaac tcgtcgcggc cgtcgtcgcg catgacgtgc agcacgaacg ccgggttatc
     1921 gcgatcctcg tcgcgcgcgt cgtactgcgg cttgccgtcg ttgatcagaa tggccttcgc
     1981 cgtctgctgc cgcgtcgaga cgaagatgat gcggccgtcg gcgagatagt gcggcgagag
     2041 atcgtggcct tgctgggccg tcagatcgga ggcgatgagg cgccgcaggt tgtccgtggc
     2101 gatctcgtac tcccagatgt tccacttcgg ctggtcgggg tcgtcgagcg cgaggccctc
     2161 gtcgacgggg ccgcgcatcg cgaacaagac tttgcggccg tcgctcgaga tctcgacgcc
     2221 cgagacgtcg cccgtcccgt tcgtgaaacg catcgtgacg ttgcgctccg gtgccgtcgg
     2281 cgaagcgcgg tcgcgaatgt acaggtcggt gccgaccgcg aaacgcagga tgtcgcgcaa
     2341 gtcggcgttg acttgcagct gatcgtcctc gtcgttcagc gggcccttcg tgtacgcgat
     2401 cggaaagtcc ggcgccaccg gatcgggatc ctgcccggtg ccgacgccga cgctgccgtc
     2461 gccgtcgccg ctgcaagccg tgacggccgc ggcgatcaac gcgagcacga gcactcggct
     2521 gtgcctcgag caagcctgga agacgttcct cgtgtcgtag ctcatggaag gtccctctcc
     2581 attaactggc gctgtcgagg gtcgatccag gcttcaattg acgcgtcaac ggcaaactcc
     2641 gaatgcagcg ccacgagcaa tcctttcgct cgtggcgggc ttgcataatc tcgcgaccgc
     2701 tctcgtcgcg atgcgacgta atgccggcgc ccggtaatca tgggcatcga cctctacgcc
     2761 taccgttaac gtgatcacag aacttgcccg gatcacctcg cgttcgaagt gtgatcgacg
     2821 tctccatgtc ctcacacttc actcccgcgt cgtcgcgaga gacttcacct aatcgcaccg
     2881 tttcacgctt cggcgaattc gaacggtgct tccgatcacc gcgaggagct tacacgtcga
     2941 ctaggctgct tccgaaaccg tcgccaacga gagacgtctg tgatttccca cattacgcag
     3001 caattttttt tgtgttttta tgcgacccga attcgcacct tgggctcaac cgacgggacg
     3061 attgcgatga gtaaccggat gcacgttgcg ccgttagcaa tcctcggcaa ccacgcgttg
     3121 ctgtggcgaa tcttcgtggc gcttgcgctg ctcttgtgcg tcttgtgcgg tgcgtcgatc
     3181 gcgctggccg ggccgcgaga gcaagcggcg cgcatgcacg accgtctcgc cggcgtgccg
     3241 gccgacgaag ctacgttgaa cgcgatggct gcgacgctgc ccggcaaccc gacggccgcc
     3301 gcgaacatcg cgatgagcaa cccgaacttc tacgcggtga cgctgaagaa cttctcggcg
     3361 ccctggacga accgcgatca gaacgcgttc gtgccgctca acgactacat ggcgctcgtc
     3421 atgggcatgg tgcgcgacga cgtgccgttc aaccagatcc tgacggcgaa cttgctctac
     3481 acgggaccgg ccagcgtgac gccggcgccg tcgccgacga gcaatgccca cttcgaggcg
     3541 ctcgaggcgc gcatgcgcga ttcgacgttc gaccagagcc agctcgtgca gaccacacag
     3601 acggcggtct acggcacgcc tgccgctgcg acggccggcg cgatcacgac gcgcgccgcc
     3661 gccgaggcgt tcttcatcgc cggcacgaac cgagcgatgt tccgcttcac gctcatgaac
     3721 cacatgtgca tggacatgga gcaagtgcac gacacgagca tcgcgccgga ccgcatccgc
     3781 caggacgtct cgcgcagccc gggcggcgat agccgcgtct tcctgaacaa ctgcataggg
     3841 tgccacgcgg gcatggaccc gctggcgcag gcattcgcgt actacgactt cgacgaaacg
     3901 tcgcagcggc tggtctacga ctcgagcgcc gtgaattcga agtacttcaa caacgacgcg
     3961 acgttccccg acggtttcgt gacgcccgac gactcctggc acaaccactg gcgccagggt
     4021 cagaacgcgc tgctcggttg gaacgcggcc cttcccggcc agggcaacgg cgccaagtcg
     4081 ctcggcgagg agctcgccgg gacacaggcg ttcgcgcagt gccaggtcaa gaaagtcttc
     4141 aaggccgtct gcctgcgcga tcccgtcgac gcgaacgacc gtaatcaaat cgacgcgatg
     4201 acgaacacgt ttcggaacgc ctacaacttg aagcaggtgt tcgccgagag cgctgggtat
     4261 tgcatgggca actagcccac gcaccaggag tgacgacgat gcagtacgca agcttgatgt
     4321 cagtcgagtc gcccggcgtg ggcgccacga agcgcggccg ttcgctcccg acgcggctcg
     4381 ccggcggcgg cgccgtgctc gtcgcggcca tgctcgccgc gtgcagcggc ggcagcggcg
     4441 ctgacgtcga agagacgccg gggcagcccg tcgacgtcac gtcgtcgaac tacaacggcc
     4501 cgcccccttc gacggcggac gtgcagagct tcaagatcaa tctctgggac aacgtccagg
     4561 cgaacaaccg ctgcggcacg tgccacagcg atacgggcgg tcaagcgccg atgtttgcgc
     4621 gccgcgacga catcaacctc gcgtacgcgg ccgccaacgg cgtcgtgacg ctcgcatcgc
     4681 cgcaagactc gcagatggtc gcgaaggtcg gcggcggcca caactgctgg ctcgcgagcg
     4741 acgccgcgtg cggcgacatc ttgacgacgt ggatcacgaa ctgggccgga tcgacggcct
     4801 cgacggccgc gcgcaagatc gagctcgagc cgccggtgct tcgcgacccc ggccagagca
     4861 agaacttccc gcccgaccag ggcgcgttgt tctcgacgac cgtatacccg atcctcgagc
     4921 aacactgctc ggagtgccac gcgtcgaata cggcgctcaa gcagcagccg ttcttcgctg
     4981 aaggcccgcc tagcgacagc gatgccgtcg agctcgcgta cgaagccgcg aaaccgaaga
     5041 tgaacctcga cgatccggcg atctcgcgcc tcgtcgtgcg gctgcgcaac gaatcgcaca
     5101 actgctggac gtcgagctgc acgaacgacg cgaacgtcat ggaaacggcg atccagaatt
     5161 tcagcaacaa cgtcacgccg acgtccgtgg acccgagctt ggtgacgagc aaggccacga
     5221 cgctctacga gggcacgatc gcgtcgggcg gtaaccgcta cgaggcgaac gtcatcgcga
     5281 tgtacgagtt caagacgggc caggactgcg gcacgtcgat cacgggcgtg tgctcgctcg
     5341 cttacgacac gagcggcgtc gatcccgcga tggacctgac gctgtcgggc aacgtgcagt
     5401 ggttcggcgg ctggggcttg aacttcgccg gcggcaaggc gcaggcgagc acgagctcga
     5461 gcgccaagct caagaacctg atcctgtcca cgggcgaata ctcgatcgag gcctgggtcg
     5521 cgcccggcaa cgtcgtgcaa gaggacatgc gcatcgtcag ttactcggcg agcttgacga
     5581 accgcaactt caacctcggc cagacgatgt acgactacga cttcttcacg cgcagcagcg
     5641 tcagcagcgc caacggcgat ccggcgctgt cgacgccgtc cgccgacgaa gtgctgcaag
     5701 cgacgctgca gcacgtcgtc gcgacgttcc atcctgcgac gggccgccgc atctacgtca
     5761 acggcgtgct cgtggcgacc gacgccgccc cgggcggcac gatcacgaac tgggacacgt
     5821 catacgcatt cgtgctcggc aacgaggtgt cgggcaaccg catgtggaac ggcgtcattc
     5881 gcctcgtggc gatccacaat cgcgcgctga cgcaagcgca gatccagcaa aatttcgacg
     5941 cgggcgtcgg cgagaaattc ttcctgatgt tcggcgtcga gcatctcacg aacatcaacg
     6001 acagcttcat cgtcgtcgag gccgcgcagt tcgacagcta cggctatctg ttccgcacgc
     6061 cgttcttcat cagcttggac ggcacggcgc aaccggccgg cctcaacatc cgcggcatgc
     6121 gcgtcggcct gaacggcgcc gaagcgcacg tcggccaggc gttcgcgaat ctcgacacgc
     6181 agatctcggg caccgtctac acgcccggga cgggccagac gctgctgaac ctcggcaccg
     6241 tgttgccgct cgagaagggc cccgacgagg acgagttctt cttgacgttc gatacgctcg
     6301 gcgcgaacac gtactcgcgc ccgcccccgg ccgtgccgcc ggcgccgacg cccgaagacc
     6361 tgcctcccgc gtcggcgatc ggcgtgcgca ccttcgacga gatcagcgcg tcgatcgcga
     6421 ctctcacggg cgtcagccaa aacgatccgg gcgtgcgagc ggcagtcgac gaggttcggc
     6481 aatcgctgcc ggcggtcccg tcgctcgagg cgtacctatc gtcgcaccag gcggccgtcg
     6541 ggcagctcgc agtcgaatac tgccacgcgc tgatcgagga cccggcgctg cgcgcggcga
     6601 cgttcccggg cttcaatttc acgacgacgc cggcggcggc gttcgcgaac gagaacgccc
     6661 tcttcgatcc attgctcgat cggacgctcg gtttggtcca gctcggccac cagccggaca
     6721 agaacgccgt tcgcacggag ctgagccgcc tggtcaatgg ctaccccgac gacgcggggc
     6781 tctccggagt ggtgcgcccc ggcctgctga acgcgctgcc gccggggcag gcgaacgacg
     6841 agcagcgaac gcgcgcaatc gcgaaagcgg tctgcgcgtc cgtggtcggc agcgccgcga
     6901 tgttgatcca gtgattcgcg ctttgcagca gaggtaacgg ccatgctcat cagagtcaaa
     6961 cgcaagctcc gccatcccga agcgccgcaa cggcatgaaa accatcgccg cccgatgacg
     7021 cgccgcgagc tgatcgcgca aggcttcatg acgggcggcg cggccgtgct cacgaccggg
     7081 ctgttcagcc tcttcgcgaa tccgcgcgcg gcccttgcgc agctcgccga ggacctggcg
     7141 gcggacgcag gcgtgctcgg ctgcggcgtc gatcggaccg gcacgaagat tccgttcatc
     7201 tgcttcgacc tcgcgggcgg cgcgaacctc gcgggctcga acgtgctcgt cggcaggggc
     7261 ggggcgctcg gccagctcga cacgctgtcg acggccggct acagcaagct cggcttgccg
     7321 ggcgaccagg tccccggcgt cgcggaagcg gcgccgagtg cgaccggcac gagcaacggc
     7381 ctccacgccg acacgagcct cggtctcgcg ttccacagcg acagcgcgtt cctgcgcggc
     7441 atgtatgcga gcttcaaggg agatgcggcc gctatcgccg cgggcaccaa cggcgccgtg
     7501 atcccggccc gttccgagaa cgacacgggc aacaatccgc acaacccgct ctacggcatc
     7561 cagcgcgcgg gcgcggaggg ttcgatcctg acgttgatcg gctcgcagaa cacggactcg
     7621 ggcggcaact cgatggcgcc ggccatgatg atcaacgcgg agttccggcc gacgaaggtc
     7681 gaccggccga gcgacgtcac gggcctcgtc ggcacgagcg accggccgaa gcgattgagc
     7741 caccaagacg aggtcgccgt gacggaggcg atctatcggc tcagcaagcg acggatcgac
     7801 aacaacgtct cgacgggcct gatcggcccc gacccggtca gagccccgat cacgcgtgac
     7861 caggtcgtcg aggagctcgt gaagtgcggg tacctcgacg ccgcgtacac gaaggaaaag
     7921 ttcggcaacg tcgtgttgaa tccggccgcg gacttggaca tcgtcggccc gaccggcatt
     7981 ttcaccgacg ccgagttcaa cgcggggggc accgacggca acgagttccg caagacggcc
     8041 tcggtcatga agatggtcat cgacggctac gccggcgccg gctgcatcac gatgggcggc
     8101 tacgactacc acggcggcat gcgccaggaa ggcgagacga aggacttccg cgcgggtcgc
     8161 tgcatgggcg cggtcctcgc atacgcgatg cgcaggggca agcccgtgat gatgtacgtg
     8221 ttcagcgacg gctcgctgtc gagcaacggc gtcatcgaca actcggtggc gggccgcggc
     8281 aagggcgagt gggtcagcga caaccaggac atggcggcgc cgttcttcct cgtctataac
     8341 ccgacgggtc agcgccccac gctgctgacg agccagaaca tgaccgccgc gcagcatcag
     8401 cagctcggcg cgttctcggc ggacggcaac gtcgtgcgct cgggcacgcc cggcgcgaac
     8461 aacgtcaacc tgctcgtcca catgatcctc ctgaactaca tggcgctgca cggtgagcaa
     8521 ggccagttca cgacgctgtt cccgaaccat ggcctcggcg acccggcgct gctcgaccgg
     8581 ttcattgcgt tcgctccgat cgtctagcgg tcggcaagct cgaggagttc ttagcggttc
     8641 cctcgttcga tacgacggtt actcggccac ggatggctga gtaaccgttg ttcttttttg
     8701 tcgcgccgtt ttcacttaac gtgagcggtg cacgggctcg acccgcgaat cccggcaacg
     8761 atttcaccct tgagcgacga accgaccgca atgactgccg ccgaagcgct gcgcgagctg
     8821 ctcgcctgcc cgcgctgcga cgcgccgctt accgaagccg gtgaagcgtg gcgctgcggc
     8881 ggctgcgagg tcgagtttcc gcgcctcgcg ggcattccgt ggttgttcgc cgagccgaac
     8941 gcggcgctcg ccgaatggcg cgtccggctg catttcgctc tgcagaagct cgagcgcgat
     9001 cggcagaaca tcgcggcgtc actcgcgggc gcaccgctgc ggacggcgac gcgcgcacgt
     9061 ctcgagagcc tcgagcgcgc gacccgcgag cacggcgagc gcctgcgcgc actgctcgcg
     9121 cctctcgagc tcgatcggca cgccgcgagc tacgaggtcc atctcgcgct gcgcacgcgg
     9181 ttaccggccc atcaaggatt gacgacctac tacgcgaaca tccatcgcga ctggtgctgg
     9241 ggcgaagagg agaacgccgc ctcgtacgag acgctggccg cgtggctacg ggccgcgccg
     9301 ccgagccgcg ccctcgtgct cggcgcgggc gccggccgcc tcgcctacga cttgcacgtg
     9361 caaacggcgg cgcgcacgac ggttgcgctc gatttcaacc cactgctctc gatcgtcgct
     9421 gacaacgtca cgcacggccg ggcgatcgag ctctacgagt ttccgatcgc gccgcgcggc
     9481 gacgcagccc tgctgcggac gctttcggca ccctcgccag cccgcgccgg gctcgccttc
     9541 gtgctggcgg acgcgcatcg gccgccgctt cggcgcggcg cgttcgacac cgtcgtcacg
     9601 ccgtggctcg tcgatatcct gcccgagcgc ttcgacatcc tatgcgcgcg cgtcaacgca
     9661 ctactgggcg acggcggaca ctggctgaac
//
LOCUS       DASSNN010000538         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_327314, whole genome shotgun sequence.
ACCESSION   DASSNN010000538 DASSNN010000000
VERSION     DASSNN010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327314"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VFL84_10050"
     CDS             complement(<1..789)
                     /locus_tag="VFL84_10050"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HET7609008.1"
                     /translation="MSDQTQWAFPAELQPKSDEVAFDLRAALDAVVALRVEVPEDAFT
                     ASILGTERTGNGVVINDEGLVLTIGYLITEADSIWLTSHSGNAVPGHALAYDQTTGLG
                     LVVPLGKLDLPPLPRVKLRSASLDRDVFVIGHGGRGHALRARVFARREFAGYWEYLLD
                     EALFTTPPHPEWSGAALLDGRGRLIGIGSLFVQESDGDDTVKGNMFVPSELIDPILDD
                     MLRIGRADRPARPWLGIYTAETKDGITIQGLATGGPAERAGVQLG"
     gene            919..1599
                     /locus_tag="VFL84_10055"
     CDS             919..1599
                     /locus_tag="VFL84_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7609009.1"
                     /translation="MRVPTLLLLLFAVAVPASAQVPPSAAERGAYQGLFLAAARGDAA
                     EIARLAAAGSAVDVRDSYARTPLHVAAYFGQQEAMRALAKAGADPNALERDRYDIVTI
                     AAVANDVRSLDTALAIGARATNVTSRYDGTALIAAAHLGHDTVVRTLIRAGAPLDHVN
                     NLGWTAVIESIVLGDGGPRHVATLKALVDAGADVNLADRSGTTPLALARGRGFREMVV
                     LLERAGAR"
     gene            complement(1610..>2094)
                     /locus_tag="VFL84_10060"
     CDS             complement(1610..>2094)
                     /locus_tag="VFL84_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609010.1"
                     /translation="SAAAHRHAVRRRAAHSPTPTIETKRSLVAAHRCPACGGTNLQRS
                     SIRRSEAGTHAFRSPYRCKDCRHRFWVISRRTRIGVLSIAAGLAAVAIVAAGVLLLPA
                     YVPTAVAVDHDIEEWSPPAPMQWTVLTPKGMAAAVLEPNEVLSGGGEQGVFRRLGDVR
                     "
BASE COUNT          309 a          745 c          735 g          305 t
ORIGIN      
        1 gccgagctgc acgcccgcgc gctcggcggg accgccagtg gcaaggccct ggatcgtgat
       61 gccgtctttg gtctccgccg tgtagatgcc gagccacggc cgcgcgggac gatcggcgcg
      121 gccgatgcgc agcatgtcgt cgaggatcgg gtcgatcaac tcgctcggca cgaacatgtt
      181 gcccttgacc gtgtcgtcgc cgtccgattc ctgcacgaac agcgagccga tgccgatcag
      241 ccggccgcgg ccgtcgagca gcgcggcgcc gctccactcc ggatgcggcg gcgtcgtgaa
      301 cagcgcctcg tcgagcaggt actcccagta gcccgcgaac tcgcggcgcg cgaacacgcg
      361 cgcgcggagc gcgtggccgc ggccgccgtg gccgatgacg aatacgtcgc ggtcgaggct
      421 tgccgagcgc agcttaacgc gcggcagcgg cgggagatcg agcttgccga gcggcaccac
      481 gagcccgagc ccggtcgtct ggtcgtacgc gagcgcgtgg ccgggaacgg catttccgga
      541 atggctcgtg agccagatcg agtcggcctc ggtgatcaga tagccgatcg tcagcacgag
      601 gccttcgtcg ttgatgacga cgccgttgcc ggtgcgctcg gtgccaagga tcgaagcggt
      661 gaacgcgtcc tcgggcactt cgacacgcag ggcgaccacc gcatcgagcg ccgcgcgaag
      721 gtcgaacgcg acctcgtcgc tcttcggctg gagctcggcg ggaaacgccc actgggtctg
      781 atcggacatg tgttcggggc cgtgccggtt ggagtcaaaa cgatactggg tcggccattt
      841 tggcccacgc tcttgggcac cgcgcgcggc cgatcgttca tgccggtaga atcccgctcc
      901 cgtctccggg tcttgcggat gcgcgtcccg acgcttttgc tccttttgtt cgccgtggcc
      961 gtcccggcgt cggcgcaggt gccgccgtcc gccgcggagc gcggcgcgta ccagggactg
     1021 ttcctggcgg ctgcccgtgg cgacgccgcc gagatcgcgc gcctcgcagc cgcgggcagc
     1081 gccgtcgatg ttcgcgacag ctacgcccgc actccgctgc acgtcgcggc gtacttcggc
     1141 cagcaggagg caatgcgcgc gctcgcgaag gcgggcgcgg acccgaacgc actggagcgc
     1201 gatcgctacg acatcgtcac gatcgcggcg gtcgcaaacg atgtccgctc gctcgacacg
     1261 gcgctcgcaa tcggcgcgcg cgcgaccaac gtgacgagcc gctacgacgg caccgcgctg
     1321 atcgccgctg cgcacctcgg ccacgacacc gtcgtgcgca cgctgatccg agccggcgct
     1381 ccgctcgacc acgtcaacaa tctcggctgg acggccgtca tcgaatcgat cgtgctcggt
     1441 gacggcgggc cgcgccacgt cgcgacgctg aaggcgctgg tcgacgctgg cgccgacgtc
     1501 aatctcgccg accgcagcgg cacgacgccg ctcgcgctcg cgcgcgggcg cggcttccgc
     1561 gagatggtcg tcttgctcga aagggcgggc gcccgctgaa ccagccgggc taacgcacgt
     1621 cgccgaggcg gcggaagacg ccctgctcgc cgccgccgct cagcacctcg ttcggctcca
     1681 gcacggcggc cgccatgccc ttcggcgtca gcacggtcca ctgcatcggc gcaggcggcg
     1741 accactcctc gatgtcgtga tcgaccgcaa ctgcagtcgg gacatacgcg ggaagcagca
     1801 gcacgccggc cgcgacgatg gcaaccgccg cgagaccggc ggcgattgac agcacgccga
     1861 tgcgcgtcct gcggctgata acccagaagc gatgacggca atccttgcac cgatacggcg
     1921 agcggaacgc gtgcgtcccg gcctccgacc ggcgaatgct cgagcgctgc aggttcgtgc
     1981 ctccgcatgc gggacagcga tgcgccgcaa cgaggctgcg ctttgtctcg atggtcggcg
     2041 ttgggctatg cgccgcccgg cgccgcacgg catggcgatg ggcggcggca ctcg
//
LOCUS       DASSNN010000539         5861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1477672, whole genome shotgun sequence.
ACCESSION   DASSNN010000539 DASSNN010000000
VERSION     DASSNN010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477672"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /gene="gspF"
                     /locus_tag="VFL84_10065"
     CDS             <1..1059
                     /gene="gspF"
                     /locus_tag="VFL84_10065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HET7609011.1"
                     /translation="KRQFSLFKGISGLDLALLTRQLATLAKAGLPLDEALLAVSEQTE
                     KPRLKSIILGVRAKVLEGHSLASGLDEFPHAFPPVYRATVMAGEQAGQLDSVLDRLAD
                     YTESRHGLRQKVTQAMVYPVLLTTIALIIIVVMLIAVVPKVVGVFETTGQELPVMTRA
                     LIAISNFVQNWWFLLIVGVVGVVFLVVRALRNDDVRRRVHLWQLRAPIFGRVTRGLNT
                     ARFTRTLSILTASGVPALEALKISASVIVNLPMRAAVEDAAVRVREGGAIGRSLAQSK
                     LFPPMSIHLISSGEASGELDKMLERAANHQESEMDNLIATMLSVLEPLLIVIMGLVVL
                     AIVMAILLPIFQINQLAR"
     gene            1198..1479
                     /locus_tag="VFL84_10070"
     CDS             1198..1479
                     /locus_tag="VFL84_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609012.1"
                     /translation="MKVKESEKKKEEEEIEDDDEESEEEEGSTEAAAETDNVGDASVE
                     INVEELIHELEGDGVARPGREQAAKRRLEELMEKKRSKRELEDFDDYEV"
     gene            complement(1481..4588)
                     /locus_tag="VFL84_10075"
     CDS             complement(1481..4588)
                     /locus_tag="VFL84_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HET7609013.1"
                     /translation="MQPANGTNGVYAELHCLSNFSFLRGASHPQELVRTAAELGYAGL
                     ALTDECSVAGVVRAHTAAKELPLKLVVGSELVTDSGLKVVALADDRSAYAALCGLISR
                     ARRAAPKGEYLLRSDDVAECLRDHGLLLWLPSFADTEADARTGRWLKERFEGRLWVAV
                     ELLNLGSDRRVLQQARALGYELAVPLVASGNVHMHSRERRMLQDTLTAIRRKVPIHEL
                     GFELHSNAERSLRPVHELVRRYPPELLRETLAILERVNFSLAELRYEYPYELIPAGET
                     PTSYLRALTERGCRWRWPEGESPKVRSLIEHELTLIAELRYEAYFLTVHDIVSYARSI
                     GILCQGRGSAANSVVCFCLGITEVDPDRMQTLVERFISKERNEPPDIDVDFEHERREE
                     VIQYIYRKYSRERAALAATVITYRGRSAIRDVGKALGIEELKVGALASSLQWWESGVI
                     DEQRIREAGLDPKSRKVWRWIKLAESLLGFPRHLSQHVGGFVIAERPIHELVAVENAA
                     MPERTVIQWDKDDLEDLGLMKVDVLGLGMLSAIRRSFELIERFDGRKLTMANVPAEDP
                     AVYRMIQKADTIGVFQIESRAQMAMLPRLKPKNYYDLVIEIALVRPGPIQGDMVHPYL
                     RRRNGEEKVDYPSPEVENVLKRTLGVPIFQEQVMQLAIVAADFTPGEADQLRRAMAAW
                     KHRGGLEPFEAKLKTRMRAKGYSEEFANRIFQQILGFGDYGFPQSHSASFALLAYVSA
                     WLKHYEPAAFCCALLNSQPMGFYAPQQLVRSARDHGVEVRRIDVNHSDWESTLERGAD
                     GRTAVVRLGMRLVKGLASESAARVCAARAAAGFTSVQDLTERARLNAKDLGVLASAGA
                     LRTLASNRHRARWEVAGVTQPTELLKQVRFAEALPILRRPTEGEDIAADYAHVGVSIR
                     RHPLILLRNALDQAGIQTAREVGDLDPGARVRTAGLVITRQRPSAASGVTFVTLEDET
                     GFLNLVVWERLAERDRRALLGARLLGVVGVVQKESGVLHVIAEQLIDHSALLGGLVTQ
                     SRDFH"
     gene            complement(4595..>5861)
                     /locus_tag="VFL84_10080"
     CDS             complement(4595..>5861)
                     /locus_tag="VFL84_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HET7609014.1"
                     /translation="IASGTKLQTALALEASLRVLERSPQSERKSLESLAAWAGALTPA
                     VSVEAPESLLLEVAGSLKLFGSLDAIKAKLRTELARRYRDFRFCAAPTATAALWLARA
                     ASADVLQWRQLAGSLAALPLKVTRWPPDVQALLWDLGLRTVGECARLPRDGFARRVGR
                     SYLLELDRAFGRSVDLRAEHKTPRTWSSVAELSEESVDTAIFIEAVEHLLDDLCAELE
                     QRQAEIVSLELAFEHLHRPPTIERFDLREPTLDLKRLLRLVEDRLERVALPVPAVALR
                     MASGVFMPLERSNADLFEAKPPAERANALLERLQGRFGTSAVYGLRAVAEHRPEKAWA
                     KAPECGGRPADVPAEARPLWLLQTPVAIEARKDSLELVSGPERIESGWWDEQDVARDY
                     YTAKNARGQKLWVFRDHRTRSWFLHGLFG"
BASE COUNT         1033 a         1938 c         1900 g          990 t
ORIGIN      
        1 aagcgccagt tctcgctgtt caagggtatc tcgggcctcg acctcgcgct cttgacgcgc
       61 cagctcgcga cgctcgcaaa agccggcttg ccgctcgacg aggcgctgct tgccgtcagc
      121 gagcagaccg agaagccgcg gctgaagagc atcatcctcg gcgtcagggc caaggtactc
      181 gagggtcact cgctggcatc gggcctcgac gagtttccgc acgcgttccc gcccgtctac
      241 cgcgccacgg tcatggccgg cgagcaggct ggacagctcg attcggtgct cgaccggctc
      301 gcggactaca ccgagtcgcg ccacgggctg cggcagaaag tcacgcaggc gatggtctat
      361 ccggtcctgc tgacgacgat cgcgctgatc atcatcgtcg taatgctgat cgcggtcgtg
      421 ccgaaggtcg tcggcgtctt cgaaacgacg gggcaggagc tgcccgtcat gacgcgggcg
      481 ctgatcgcga tctcgaactt cgtccagaac tggtggttct tgctgatcgt cggcgtcgtc
      541 ggggtcgtgt ttctagtcgt ccgagcgttg cggaatgacg acgtgcgccg ccgcgttcat
      601 ctgtggcagc tgcgcgcgcc gattttcggc cgcgtcacgc gcgggctcaa cacggcacgg
      661 ttcacgcgca cgctgagcat cctgactgca agcggcgtgc cggcgctcga ggcgctcaag
      721 atcagcgcct cggtcatcgt gaacctgccg atgcgcgcgg ccgtcgagga cgcggccgtg
      781 cgcgtgcgcg aaggcggcgc aatcggccgc tcgctcgctc aatcgaaact gtttccgccg
      841 atgagcatcc atctcatctc gagcggtgaa gcgagcggcg agctcgacaa gatgctcgag
      901 cgggccgcta accatcaaga aagcgagatg gacaacctga tcgctaccat gttgagcgtc
      961 ctcgagccgc tcttgatcgt catcatgggc ctcgtcgtgc tggcgatcgt catggccatt
     1021 ctgttgccga tcttccagat caaccagctc gcgcgctgag cgtgcgcccg cttttgcgcg
     1081 gggtgtgcgc tcgatcacac aaaggcgagc cgaggggtat tgccaaccat taggggcggg
     1141 tttataaaag cgccgactgc gggttcaacc gactcgatct cgatgcggag gggaggaatg
     1201 aaggtaaagg agtcggagaa gaaaaaagaa gaagaagaaa tcgaagacga cgacgaggag
     1261 tccgaggaag aagaaggcag cactgaagct gcagcggaaa cggataacgt cggcgacgct
     1321 tcggtcgaga tcaacgtcga agagctcatt cacgagctcg agggcgacgg cgtcgctcgt
     1381 cccggtcgcg agcaggccgc gaagcgtcga ctcgaagagc tcatggagaa gaagcgctcg
     1441 aagcgcgagc tcgaggattt cgacgactac gaagtttgac tcaatgaaag tcgcgcgact
     1501 gcgtgacgag cccgcccaaa agcgcactgt gatcgatcag ctgttccgcg atcacgtgta
     1561 gcaccccgct ctctttctgc acgaccccga cgacgccgag caatcgcgct ccgagcaagg
     1621 cgcgccggtc gcgctcggcg agccgctccc agacgacgag gttcagaaag cccgtttcgt
     1681 cctcgagcgt cacgaacgtg acgccggacg cggccgacgg ccgttgccgc gtgatgacga
     1741 ggccggccgt gcggacgcgc gcgccgggat ccaaatcgcc gacctcgcga gccgtctgga
     1801 tgccggcctg atcgagcgca ttcctgagca aaatcaacgg gtgacgacgt atgctgacgc
     1861 cgacgtgggc gtagtccgcg gcgatgtcct cgccttccgt cggccggcgc aggatcggca
     1921 gcgcttcggc aaaacgcacc tgcttaagta gctcggtcgg ctgcgtgacg ccggccactt
     1981 cccagcgcgc gcgatggcgg ttgctcgcga gcgtacgaag cgcgcccgcc gacgcgagca
     2041 cgcccagatc cttcgcgttg agccgcgctc tttcggtgag atcctgcacg ctcgtgaacc
     2101 ccgccgcggc acgcgcggcg cacacgcgcg ccgcggattc gctcgcgagc cctttgacga
     2161 gccgcatgcc gagccgcacg acagcagtgc gcccgtcggc tccgcgctcg agcgtggatt
     2221 cccagtcgct gtgattgacg tcgatgcgcc gcacttcgac gccgtgatcg cgcgccgagc
     2281 gcacgagctg ttgcggcgca tagaagccca tcggctggct gttcagcaac gcgcagcaaa
     2341 atgccgccgg ctcgtaatgc ttgagccacg ccgaaacgta cgcgagcagc gcaaagctcg
     2401 cgctgtgact ctgcggaaat ccgtagtcgc cgaagccgag gatttgctga aagatgcggt
     2461 tcgcaaattc ttcgctgtat cccttcgcgc gcatgcgagt tttcagcttc gcctcgaatg
     2521 gctcgagccc gccgcggtgt ttccacgcgg ccatcgcgcg ccgcagctga tcggcctcgc
     2581 ccggcgtgaa atccgcggcg acgatcgcga gctgcatgac ttgctcttga aagatcggca
     2641 cgccgagcgt ccgcttcaaa acgttctcga cctcggggct cggatagtcg actttctcct
     2701 cgccgttacg gcgccgcaga tacggatgca ccatgtcgcc ctggatcggc cccggccgga
     2761 cgagcgcgat ttcgatgacg aggtcgtagt agttcttcgg cttgagccgc ggcagcatcg
     2821 ccatctgcgc tcgcgattcg atctgaaaga cgccgatcgt gtcggccttc tgaatcatgc
     2881 gatagacggc cgggtcctcc gccggaacgt tcgccatcgt gagcttgcgg ccgtcgaacc
     2941 gctcgatgag ctcgaagctg cgccggatcg cgctcagcat gccgagcccg agcacgtcga
     3001 ctttcatgag gccgagatcc tcgagatcgt ccttgtccca ttgaatgacc gtgcgctccg
     3061 gcatcgcggc gttctctacg gccacgagct catgaatcgg ccgctcggcg atgacgaaac
     3121 cgccgacgtg ctgcgacaga tgcctgggga agccgagcag cgattcggcc agcttgatcc
     3181 agcgccagac cttgcggctc ttcggatcga gccccgcctc gcggatgcgc tgctcgtcga
     3241 tgacgccgct ctcccaccat tgcagcgagc tcgcgagcgc gccgaccttg agctcctcta
     3301 tgccgagcgc cttgccgacg tcgcggattg cgctgcggcc gcgatacgtg atgacggtgg
     3361 ccgccaacgc ggcccgctcg cgcgagtact tgcgatagat gtattgaatg acctcctcgc
     3421 gccgctcgtg ctcgaagtcg acgtcgatgt cgggcggctc gttgcgctcc ttcgagatga
     3481 agcgctcgac caaggtctgc attcgatcgg ggtcgacctc ggtaatgcct aagcagaagc
     3541 agacgacgga gttggccgcc gacccgcggc cctggcacag gatgccgatg ctgcgcgcgt
     3601 agctgacgat gtcgtgcacg gtcaggaaat acgcctcgta gcgcagctcg gcgatcagcg
     3661 tgagctcgtg ctcgatcaac gagcgaacct tcggcgactc gccttccggc caacgccacc
     3721 ggcagccgcg ctccgtcaac gcgcgcagat agctcgtcgg cgtctctccg gccggaatca
     3781 gctcgtacgg atattcgtag cgcagctccg cgagggaaaa attcacgcgc tcgagtatcg
     3841 cgagcgtctc gcgcagcaac tcgggcggat aacggcggac gagctcgtgg acgggcctca
     3901 aggatcgctc ggcattcgaa tggagctcga agccgagctc gtgtatcggg accttgcgcc
     3961 gaatcgcggt cagcgtgtct tgcagcatgc ggcgctcgcg gctgtgcatg tggacgttgc
     4021 cggacgcgac gagcggcacg gcgagctcgt agcctaaagc ccgggcttgt tgcagcacgc
     4081 gccggtcgct ccctaagttc aagagctcga cggcgaccca gaggcggcct tcgaagcgct
     4141 ccttgagcca gcgtcccgtg cgcgcatcgg cttccgtgtc cgcgaagctc ggcagccaca
     4201 ataggagccc gtggtcgcgc agacattcgg cgacgtcgtc gcttcgtaac aggtattcgc
     4261 ctttcggagc cgcgcggcgc gctcgactga tgaggccgca cagcgccgca tacgcggatc
     4321 gatcgtcggc cagcgcgacg actttgaggc cgctatccgt gacgagctcg ctgccgacga
     4381 cgagcttcag cggcaattcc ttggcggccg tatgcgcgcg cacgactccg gccacggagc
     4441 actcgtcggt caacgcgagg cccgcatacc cgagctcggc cgcggtcctc acgagctcct
     4501 gtggatgcga cgcgccgcgc aggaacgaga aattactgag gcagtgcagc tcggcgtata
     4561 cgccgttcgt gccgtttgca ggctgcatgg cggctcaacc gaacaacccg tgcaagaacc
     4621 aagaacgagt ccggtgatcg cggaagaccc acagcttttg gccgcgcgcg ttcttggccg
     4681 tgtagtaatc gcgcgcgacg tcttgctcgt cccaccagcc gctctcgatt cgctcggggc
     4741 ccgagacgag ctcgagcgaa tctttgcgcg cttcgatggc cacgggtgtc tgcaacaacc
     4801 ataacggccg cgcttcggcg ggcacgtcgg ccggccggcc gccgcactcc ggcgctttcg
     4861 cccaggcctt ttcggggcgg tgctcggcaa cggcgcgcaa gccgtacact gccgacgtgc
     4921 cgaaccgccc ttgcagacgc tcgagcagcg cattcgcgcg ctcggccggt ggttttgcct
     4981 cgaacaaatc ggcgttgctg cgctcgagag gcatgaatac gccgctcgcc attcgcagcg
     5041 ccacggccgg caccggcagc gcaacgcgct ccaatcgatc ttcgacgaga cgcagcaacc
     5101 gctttaggtc cagagtcggc tcgcgtaagt cgaaccgttc gatcgtcggc ggccgatgca
     5161 gatgctcgaa ggcgagctcg aggctcacga tttccgcttg acgctgctcg agctccgcgc
     5221 agagatcgtc caagagatgc tcgacggcct cgatgaaaat ggcggtgtcg acgctttcct
     5281 cgctgagctc ggcaacggag ctccaggtcc gcggcgtctt gtgctcggcg cgcaagtcga
     5341 cgctgcggcc gaacgcgcgg tcgagctcga gcaaatacga acgcccgact cggcgcgcaa
     5401 aaccgtcccg cggcaaccgc gcgcactcgc cgacggtgcg gagccccaag tcccacaaca
     5461 gagcttgcac gtcgggcggc cagcgcgtga ctttgagcgg cagcgccgcc aagctgccgg
     5521 ccaattggcg ccattgcaga acgtcggcgc tcgccgctcg cgcgagccac aaagcggcgg
     5581 tcgccgtcgg cgcggcgcaa aagcgaaaat cgcggtagcg ccgcgcgagc tccgtgcgga
     5641 gcttggcctt gatggcatcg agagagccga acagcttgag gctgccggca acctcgagca
     5701 gcaagctctc aggcgcctcg acgctgaccg ccggcgtcag cgcgccggcc cacgcggcca
     5761 acgactcgag gcttttccgc tcggactgcg gcgaccgctc gagcacgcgc aaagacgctt
     5821 cgagcgccag ggccgtttgg agcttcgttc ccgaagcgat g
//
LOCUS       DASSNN010000540         4319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_470922, whole genome shotgun sequence.
ACCESSION   DASSNN010000540 DASSNN010000000
VERSION     DASSNN010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470922"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VFL84_10085"
     CDS             <1..1036
                     /locus_tag="VFL84_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7609015.1"
                     /translation="MQLQPIVHVAPDGLTAQGRWHVFAQEAEHREYANWGLGVLENDY
                     VKEDGVWKIRRHHFFTTMYTPYDAGWAKQAIPNAGPSAELPPDRPPTVAYEAYPKAFV
                     VPFHYSNPVTSAAPKAGVEVVAATATPESIGSQLTALERRVGLLEDVAQLERMNSIYG
                     YYLAHLQWDDLTGIFSPTGSIEIAMRGVYIGSASVRRNLDLYGKATDDQFGLQHNHMQ
                     FQPVIDVAPDGQSAKMRSRALSIMGQWERYSQWMGGIYENDYVKENGVWKIKRDQVFN
                     TYFVPYTVGWKDSQPRPPPGITAANPPDAPPTHPFEMYPSAFLPPFHYVHPVTGRPVE
                     WTPPAAAAAR"
     gene            complement(1044..1817)
                     /locus_tag="VFL84_10090"
     CDS             complement(1044..1817)
                     /locus_tag="VFL84_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012142997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HET7609016.1"
                     /translation="MQIDIFATVLIVIVAALLFSAFKVLREYERGVIFMLGRFYRVKG
                     PGLIVLIPVVQQMVRVDLRTKVLDVPSQDIITRDNVSVKVNAVIYFRVVDPQHAIINV
                     ENYMEATGQMAQTTLRSVLGQHELDELLAERDRLNNDIQKILDAATDVWGIKVSNVEI
                     KHVDLNENMVRAIARQAEAERERRAKVIHADGEYQAATKLVEAAAQLAREPQSMQLRY
                     LQTLTEVAGEKSSTIVFPVPTNVLDLLRGLAANETPKPR"
     gene            complement(1819..3129)
                     /locus_tag="VFL84_10095"
     CDS             complement(1819..3129)
                     /locus_tag="VFL84_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HET7609017.1"
                     /translation="MGTVTANASEVFVVELDGAITPATARYFIRSLDDAQEAGAAALV
                     VQLDTPGGLDEAMRDMIKAILASRVPVVVYVAPNGSRAASAGTYLLYASHVAAMAPAT
                     NVGSSTPVSIGGSPFPQGEPPAEGDGDAREPGPSAMERKIVNDAVAYIRGLATLRGRN
                     ADWAEMTVREAANLTAAEALEMNVIDLVAEDLDALLMALDGRAVDVAGATVTLDLEGA
                     EIERIVPDWRDELLALITNPNVAYILLMLGIYGLILEFYNPGTGVPGVTGVICLLLAA
                     YALQMLPVSYAGLALLLFGVALMVAEIVTPTVGVLGVGGVIAFVAGSIILFDSDLPGY
                     RVSIPIIAAAAVASAAVFLIGIGSAMRARRLRVATGREAMIGARAVALEDFAKRGNVR
                     AFSENWLAESPHPVRKGDELRVTGVEGLVLRVEPTTQEHVAEES"
     gene            3275..>4319
                     /locus_tag="VFL84_10100"
     CDS             3275..>4319
                     /locus_tag="VFL84_10100"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HET7609018.1"
                     /translation="MTDSTSKDKTEIDRRSFVAGALGAAAGAAALLKQSAQAQPPQGR
                     ELGTTAGAAIPPEMRRPQSLYRLEADVRDCQVTGKVPADLNGAFYRVGPDAQYPLHPK
                     TIPFDGEGHVGMFRIKNGRVDFKSRMVRNERYIANDKAGRQLFPVYRNPSQDDPSVKG
                     LSRSTANTHIINHKNYLLALKEDSPPVAMDLLTLDTIDPVYTFDGQLPSQTFTAHPKL
                     DSMTGNMVAFGYEADGFGSKTVSIFEITPQGKMVWNTKIEVPYVGMLHDFAVTEKHVV
                     FYVIPLAIDEKQMAEGGIHWSWYPGQPTYFGFVRRGGDGKDVQWIKGPTRSTRCIRRR
                     FRSTAKGTSACFAS"
BASE COUNT          763 a         1436 c         1428 g          692 t
ORIGIN      
        1 gatgcagctg cagccgatcg tgcacgtcgc gcccgacggg ctgaccgcac agggacgctg
       61 gcacgtgttc gcgcaggaag ccgagcaccg cgagtacgcg aactggggct tgggcgtgct
      121 cgagaacgac tacgtcaagg aagacggcgt ctggaagatt cgccgtcatc acttcttcac
      181 gacgatgtac acgccgtacg acgcgggctg ggcgaagcaa gcgatcccga atgcggggcc
      241 gtccgcggag ctgccgcccg atcgtccgcc gacggttgcg tacgaggcct atccgaaggc
      301 gttcgtcgtg ccgttccatt actcgaatcc cgtgacgagc gcggcaccca aggccggcgt
      361 cgaggtcgtc gccgcgacgg cgacgccgga atcgatcggc tcccaactaa ctgcgctcga
      421 gcgccgcgtc ggcttgctcg aggacgtggc acagctcgag cgcatgaact cgatctacgg
      481 ctactacctc gcgcatctgc aatgggacga tttgaccggc atcttctctc cgacgggcag
      541 catcgagatc gcgatgcgcg gcgtctacat cggctcggcg agcgtgcgcc gaaacttgga
      601 tctgtacggc aaggcgaccg acgaccagtt cggtttgcag cacaaccaca tgcagttcca
      661 gcccgtcatc gacgtcgcgc ccgatgggca gagcgcgaag atgcgctcgc gcgcgctcag
      721 catcatgggc caatgggagc gctacagtca gtggatgggc ggcatttacg agaacgacta
      781 cgtcaaggag aacggcgtct ggaagatcaa gcgcgatcag gtcttcaaca cgtacttcgt
      841 gccgtacacg gtcggctgga aggattcgca gccgcggccg ccgcccggca tcacggccgc
      901 gaacccgccc gacgcgccgc cgacccatcc gttcgagatg tatccgagcg cgttcctgcc
      961 gccgttccac tacgtacatc ccgtgacggg ccggcctgtc gagtggacgc cgccggccgc
     1021 agccgcggca cggtgagaac gcgtcagcgg ggcttcggcg tctcgttcgc cgcgagcccg
     1081 cgcagcaggt cgagcacgtt cgtcggaacg gggaagacga tcgtgctgct cttttcgccc
     1141 gccacctcgg tcagcgtctg caggtagcga agctgcatcg actgcggctc ccgcgcgagc
     1201 tgcgctgcgg cttcgacgag cttcgtggcc gcctggtact cgccgtcggc gtggatgact
     1261 ttcgcgcggc gctcccgctc ggcctcggct tgtctggcga tcgctctgac catgttttcg
     1321 ttcaggtcga cgtgcttgat ctcgacgttc gacaccttga tcccccaaac atcggtcgcg
     1381 gcatcgagga tcttctggat gtcgttgttc agccgatcgc gctccgcgag cagctcgtcg
     1441 agctcgtgct gcccgagcac ggagcgcagc gtcgtctgcg ccatctgacc ggtcgcctcc
     1501 atgtagttct cgacgttgat gattgcgtgc tgcggatcga cgacgcggaa atagatgacc
     1561 gcgttgacct tcaccgagac gttgtcgcgc gtgatgatgt cttgcgacgg cacgtcgagc
     1621 accttcgtgc gcagatcgac gcgaaccatt tgctgcacga ccgggatcag cacgatgagg
     1681 ccgggccctt tgacgcggta gaaacggccg agcatgaaga tcacgccgcg ttcgtactcg
     1741 cgcagcactt tgaatgcgct gaacagcagc gcggcgacga tcacgatcag gactgtggca
     1801 aaaatgtcga tctgcatctc acgactcctc ggcaacgtgt tcttgcgtgg tgggttcgac
     1861 acggaggacg agaccttcga cgcccgtgac gcgcagctcg tcgcccttgc ggaccggatg
     1921 cggcgactcc gcgagccagt tctcgctgaa cgcgcgcacg ttgccgcgct tggcgaagtc
     1981 ttcgagcgcg acggcgcggg cgccgatcat ggcttcgcgc ccggtggcga cgcgcagcct
     2041 gcgcgcgcgc atcgcggagc cgatgccgat caggaacacg gccgcggaag cgaccgcggc
     2101 ggccgcgatg atcggtatcg agacgcgata gccgggcagg tcggagtcga acaggatgat
     2161 cgagcccgcg acgaacgcga tgacgccgcc aacgccgagc acgccgaccg tcggcgtcac
     2221 gatctcggcc accatcagcg caacgccgaa caggagcagc gccaagccgg catagctgac
     2281 cgggagcatt tgcagcgcgt acgcggccag cagcaagcaa atgacgcccg tcacgcccgg
     2341 aacgccggtg cccggattgt agaactcgag gatgagcccg tagatgccga gcatgagcaa
     2401 gatgtaggcg acgttcgggt tcgtgatcag cgcgagcagc tcgtcgcgcc agtcgggcac
     2461 tattctctcg atctcggcgc cctcgagatc gagcgtgacc gtggcgcctg cgacgtcgac
     2521 cgcgcggccg tcgagcgcca tgagcagggc gtccaagtcc tcggcgacga gatcgatgac
     2581 gttcatctcg agcgcttcgg ctgccgtcaa gttcgccgcc tcgcgcacgg tcatctcggc
     2641 ccaatcggcg ttgcggccgc gcagcgtggc gaggccgcgg atgtaggcga ctgcgtcgtt
     2701 gacgatcttg cgctccatcg cggacgggcc gggctcgcgg gcgtcgccgt cgccttcggc
     2761 gggcggctcg ccctgtggaa acggcgaccc gccgatgctg acgggcgtcg acgagccgac
     2821 gttcgtcgcg ggcgccatcg ccgccacgtg gctcgcgtag agcaagtacg tgcccgcgct
     2881 cgcggcgcgc gagccgttcg gcgccacgta gacgaccacg ggtacgcgcg aggcgaggat
     2941 cgccttgatc atgtcgcgca tcgcttcgtc gagcccgccc ggcgtgtcga gctgcacgac
     3001 gagcgcggcc gctcccgcct cctgcgcgtc gtcgagcgaa cggatgaagt aacgggcggt
     3061 cgcgggcgtg atcgcgccgt cgagctcgac gacaaagacc tcgctcgcgt tcgcggttac
     3121 ggtgcccact cccagtagga gggccgccag gccgaacgag cagaggcgtc gcgccgccat
     3181 cgctgcatga tacgcccagc ttggggtacg gccggaagcc cggcttcgag tatcatttgg
     3241 ccaccgaatt cgactggcgc accggagacc tggcatgacc gacagcacat cgaaggacaa
     3301 gaccgaaatc gaccgccgct cgttcgtcgc gggtgcactc ggcgccgccg cgggcgccgc
     3361 ggcgctgctc aagcagagcg ctcaggcgca gccgccgcag ggccgcgagc tcggcacgac
     3421 cgccggcgcc gcgatcccgc ccgagatgcg ccggccgcag agcctgtacc ggctcgaggc
     3481 agacgtccgc gactgccagg tcacgggcaa ggtcccggcc gacttgaacg gcgcgttcta
     3541 ccgcgtcggc cccgacgcgc agtacccgct gcatccgaag acgatcccgt tcgacggcga
     3601 agggcacgtc ggcatgttcc gcatcaagaa cggccgcgtc gacttcaaga gccgcatggt
     3661 gcgcaacgag cgctacatcg cgaacgataa agccggccgc cagctgttcc ccgtctatcg
     3721 caacccgtcg caggacgacc ccagcgtcaa gggcttgagc cgcagcacgg cgaacacgca
     3781 catcatcaat cacaagaact atctgctcgc gctgaaggaa gacagcccgc ccgtcgcgat
     3841 ggatttgctg acgctcgaca cgatcgatcc cgtgtacacg ttcgacggac agctgccgag
     3901 ccagactttc acggcgcatc cgaagctcga ttcgatgacg ggcaacatgg tcgcgttcgg
     3961 ttacgaggcc gacggcttcg gcagcaagac tgtgtcgatc ttcgagatca cgccgcaggg
     4021 caagatggtc tggaacacga agatcgaggt gccgtacgtc ggcatgctcc acgacttcgc
     4081 ggtcacggag aagcacgtcg tcttctacgt cattccgctt gcgatcgacg agaagcagat
     4141 ggccgagggc ggcatccatt ggtcgtggta tccgggtcag cccacgtact tcggcttcgt
     4201 gcgccgcggc ggcgacggca aggacgtgca atggatcaag ggcccgacgc gcagtacccg
     4261 ctgcatccga agacgattcc gttcgacggc gaagggcacg tcggcatgtt tcgcatcaa
//
LOCUS       DASSNN010000541         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_471160, whole genome shotgun sequence.
ACCESSION   DASSNN010000541 DASSNN010000000
VERSION     DASSNN010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471160"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VFL84_10105"
     CDS             <1..138
                     /locus_tag="VFL84_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET7609019.1"
                     /translation="QVVVRPINFLAQSYDHRIIDGREAVLSLVAIKEALEDPARMLLD
                     L"
     gene            164..1597
                     /gene="lpdA"
                     /locus_tag="VFL84_10110"
     CDS             164..1597
                     /gene="lpdA"
                     /locus_tag="VFL84_10110"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET7609020.1"
                     /translation="MPDPQPFDVVVIGGGPGGYVAAIRAAQLGFATACIDDWTRGGGK
                     PALGGTCTNVGCIPSKALLQSSENYAHAGHAFADHGIRVEGLSIDVARMLARKDKVVA
                     QNNDGIAYLFKKNKVASFHGRGSFAGKDGDRWRIAVDGPGAATLGARHVIVATGSTPR
                     ALPGVAFDNERVLDNAGALAMAAVPKRLGVVGAGVVGLEMGSVWRRLGSTVTILEALP
                     ALLGAADEAVAKEAQKAFAKQGLAIELGVTIGNVSVGKDGVSVAYKDAAGKAQTATFD
                     KLIVSIGRVPMTTGLGAESVGLKLDARGFVDVDDECRTNLPNVWAIGDVVRGPMLAHK
                     AEDEGVAVAERIAGQHGHVDFNTTPWVIYTSPEIAWVGKTEQQLRKEGVAYRAGSFPF
                     SANGRARAIGDTTGFVKLLADAKTDRILGMHAIGPLVSELIAEGVVAMEFGASSEDIA
                     RICHAHPTLSEVTREAALSVDKRTLNL"
     gene            1659..>2076
                     /gene="zapE"
                     /locus_tag="VFL84_10115"
     CDS             1659..>2076
                     /gene="zapE"
                     /locus_tag="VFL84_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1/ZapE family ATPase"
                     /protein_id="HET7609021.1"
                     /translation="MIGADDPGQVSRRHGGLIEYLEPLHDERRATLDHAQMAALERLQ
                     QLADELVAFKAARRSALKRLFAAPPVPRGVYLWGGVGRGKSLLMDSFYATVPIRRKTR
                     VHFHAFMRDVHAGLAELKSEEDPLATVAAKIAQHFRL"
BASE COUNT          341 a          712 c          719 g          304 t
ORIGIN      
        1 caggtggtcg tgcgcccgat caacttcctc gcgcagtcgt acgaccatcg catcatcgac
       61 ggacgcgagg cggtgctgtc gctcgtggcc atcaaggaag cgctcgaaga cccggccagg
      121 atgctgctcg acctctgagt cccaacaaca actgacattt cgaatgcccg atccgcaacc
      181 cttcgacgtc gtcgtcatcg gcggcggccc cggcggctac gttgccgcga tacgcgccgc
      241 gcagctcggc ttcgcaaccg cctgcatcga cgactggacg cgcggcggcg gcaagcccgc
      301 gctcggcggc acgtgcacga acgtcggctg catcccgtcg aaggcgctcc tgcaatcgtc
      361 ggagaactac gcgcacgccg gccacgcgtt cgccgaccac ggcatccgcg tcgaaggcct
      421 gtcgatcgac gtcgccagga tgctcgcgcg caaggacaag gtcgtcgcgc agaacaacga
      481 cgggatcgcg tatctcttca agaagaacaa ggtcgcgtcg ttccacggcc gcggctcgtt
      541 cgccggcaag gacggcgatc gctggcgcat tgccgtcgac gggccgggcg cggcgacgct
      601 cggcgcgcgc cacgtcatcg tcgcgacggg ctcgacgccg cgcgcgctgc ccggcgtcgc
      661 gttcgacaac gagcgcgtcc tcgacaatgc gggcgccctc gcgatggcgg ccgtgccgaa
      721 gcggctcggc gtcgtcggcg cgggtgtcgt cggactcgag atgggcagcg tctggcgccg
      781 cctcgggtcg acggtcacga tcctcgaagc actgcccgca ttgctcggcg ccgccgacga
      841 agcggttgcg aaggaagcac aaaaagcgtt cgcgaagcag gggctcgcga tcgagctcgg
      901 tgtcaccatc ggcaacgtca gtgtgggcaa ggacggcgtc agcgtcgcgt acaaagatgc
      961 cgcaggcaag gcgcagacgg cgaccttcga caagctgatc gtctccatcg gccgtgtgcc
     1021 gatgaccacg ggcctcggcg cggagagcgt cggattgaag ctcgacgcgc gcggattcgt
     1081 cgacgtcgac gacgagtgcc ggacgaatct tccgaacgtc tgggcgatcg gcgacgtcgt
     1141 gcgcgggccg atgctcgcgc acaaggccga ggacgagggc gtcgcggtcg cggagcgcat
     1201 cgccggccag cacggccacg tcgacttcaa cacgaccccg tgggtcatct acacgtcgcc
     1261 ggagatcgcg tgggtcggca agaccgagca gcagctcagg aaagagggcg tcgcctaccg
     1321 ggccgggagc ttcccgttct cggcgaacgg ccgcgcacgc gcaatcggcg acacgaccgg
     1381 tttcgtcaag ctgctcgccg acgcgaaaac cgatcgcatt ctcggcatgc acgcgatcgg
     1441 tccgctggtg tcggagctta tcgccgaggg ggtggttgcg atggagttcg gcgcgtcgag
     1501 cgaggacatc gcgcgcatct gccacgcgca tccgacgctc tccgaagtca cgcgcgaggc
     1561 ggcgctctcc gtcgacaagc gcacgctgaa cctgtaagcc ctcgcataac cggctgcgcg
     1621 ctccgcttgc ccgttcgcgt ggatccggtg cgcattgcat gatcggggcc gacgatcccg
     1681 gccaggtctc gcgccgccac ggcggcctca tcgaatatct cgagccgctg cacgacgagc
     1741 ggcgcgcgac gctcgaccac gcgcagatgg ccgcgctcga gcggctgcag cagctcgccg
     1801 acgagctcgt cgcattcaag gcggcgcgca ggtcggcgct gaagcggctg ttcgccgcgc
     1861 cgccggtgcc gcgcggcgtc tatctgtggg gtggcgtggg ccgcggcaag agcctgctga
     1921 tggacagctt ctacgcgacc gtcccgatcc ggcgcaagac gcgcgtgcac ttccatgcgt
     1981 tcatgcgcga cgtgcatgcc ggcctcgcgg agctcaagtc ggaggaagat ccgctggcga
     2041 cggtggcggc gaaaatcgcg cagcacttcc ggctcg
//
LOCUS       DASSNN010000542         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1315617, whole genome shotgun sequence.
ACCESSION   DASSNN010000542 DASSNN010000000
VERSION     DASSNN010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315617"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFL84_10120"
     CDS             complement(<1..549)
                     /locus_tag="VFL84_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7609022.1"
                     /translation="MTRLNVSIVGAVATVLAAGAQAQRGAPNGEWPTYGGDLGHTRYA
                     PLDQIDASNFNRLEIAWRFKTDSLGPNREFNFQSTPLMVDGVVYSTAGTRRAVVALDA
                     GTGELLWIHRLDEGRRGQVAPRQLSGRGLAYWENGDDKRIIYVTPGYQLIALDARTGN
                     RVQSFGSGGIVDLKQNIDQNLDP"
     gene            731..1162
                     /locus_tag="VFL84_10125"
     CDS             731..1162
                     /locus_tag="VFL84_10125"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609023.1"
                     /translation="MRTKYRLLLLTIGLVPLTEVAAHHSPAMLYDLSKEISVTGIVTE
                     FQLGNPHMRIYLDVNNNGTIEKWLAEGGSKTQLMRAGWTGNEVKPGDKVTVRGHPPRD
                     ASQHLIHMEHLVLPDGTERFTEDIRYDQFNDRRRRPQESQP"
     gene            1159..1959
                     /locus_tag="VFL84_10130"
     CDS             1159..1959
                     /locus_tag="VFL84_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609024.1"
                     /translation="MSRARQALLGALLASCFGASAQSADPVAPFLGTWSGVFTTQQNE
                     YWTFADIQCFVGCPKEFYDHLSALLADPKNDNTPAFGLAAQSNAAWSAALDAMLTPLG
                     KQVREENKPENDPKFLNCQPYGFVREVTNPLPMQISRDGKHLLVRYEEWSLHRPIYMD
                     GRAQPTYATPSLLGHAVGRVENGALIVETTRVTPDLISDNSQVGHSGELEAVERYVVR
                     DNPRRLELTLTLTDPVTFTKPLVVTKTWLYTPDVELVQDTCNQQPGKP"
     gene            2010..>3250
                     /locus_tag="VFL84_10135"
     CDS             2010..>3250
                     /locus_tag="VFL84_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609025.1"
                     /translation="MKSAAAFLLIGASALPAATATAQNTTSEGGYEVTRSQTVADAPA
                     GYVGRKTTDRETRIGNTPETDGNSATFVMTVGGFVRECPSAEGIAAGNFEYSLTADDV
                     NTDEGETRRTHSLVSLIASLEGHVRDDGIVDYVVVDGDFTRQREGSPAEQQHVQRRFT
                     VGAQGQPDFAAMEDAVSATADVSIAIVMWMGSTLYTQAQTHWNMLDACAELAFEPATR
                     TRALGPNQSADVRVTLRTKADKAPIGGAEFQAGPLEGIGALTPRGGRTESAPIDFTYT
                     ASANPKNGNGFDIGARSRAGLASGKWQIVAATPLEGTFTQTRTMNMSGATLPPEVRAL
                     GAERYGIGTSSNYEITGRLRWTVEEGSTRPSSFGDAGSTFYVPTDGEITVKVRSDGRS
                     VAGVCTIEGGRTFRLAELPREA"
BASE COUNT          588 a         1087 c         1071 g          504 t
ORIGIN      
        1 cggatcgagg ttttggtcga tgttctgctt gagatcgacg atcccgccgc tgccgaagct
       61 ctgcacgcga ttgccggtgc gtgcatcgag cgcgatcagc tgatagcccg gcgtcacata
      121 gatgatgcgt ttgtcgtctc cgttctccca gtacgcgagc ccgcggcccg agagctggcg
      181 cggcgcgact tgcccgcgcc ggccttcgtc gagccgatgg atccagagca gctcgccggt
      241 gccagcgtcg agcgcgacga cggcgcgccg cgtgccggcc gtcgagtaca cgacgccgtc
      301 gaccatgagc ggcgtcgact ggaagttgaa ctcgcggttc ggcccgaggc tatccgtctt
      361 gaagcgccac gcgatctcga gccggttgaa gttgctcgcg tcgatctgat cgagcggcgc
      421 gtagcgcgtg tgcccgagat cgccgccgta cgtcggccac tcgccgttcg gcgcgccgcg
      481 ctgcgcctga gccccggctg ccagcaccgt tgctacggcg ccaacgatcg aaacattgag
      541 tcgcgtcatg gacggcctct gcggctcgtt ggttttccgc tgagtatagg cccgcctgct
      601 cgccgagcgt aacggccgtt gccgcccatc cgtagaacgg caaaccggct tgcatggtcg
      661 agcccggtgc gttgttgcta ccatcggcgg gccgcgtgcc gctacaacac gcgcagcctt
      721 tggagaaccg atgcggacga aataccggct gttgctattg accatcggcc tcgtgccact
      781 cacggaggtt gccgcgcatc attcaccggc gatgctctac gacctgagca aagagatctc
      841 cgtgaccggc atcgtcactg agttccagct cggcaatccg cacatgcgca tctacctcga
      901 cgtgaataac aacggcacga tcgagaagtg gctcgccgag ggcggctcga agacgcagct
      961 catgcgcgcc ggctggaccg gcaacgaggt caagccgggc gacaaggtca ccgttcgcgg
     1021 ccatccgccg cgcgacgcga gccagcatct gattcacatg gagcacctcg tgctgccgga
     1081 cggcacggag cgcttcaccg aggacatccg ctacgaccag ttcaacgacc gccgccggcg
     1141 cccgcaggaa agtcagccat gagtcgcgcg cgtcaggcgc tgctcggcgc gctgctcgcg
     1201 tcgtgcttcg gcgcttcggc gcaatccgcc gatcccgtcg cgccgttcct cggcacgtgg
     1261 agcggcgtgt tcacgactca gcagaacgag tattggacgt tcgccgacat ccagtgcttc
     1321 gtcggctgcc cgaaagagtt ctatgatcac ttgagcgcgc tgctcgccga tccgaagaac
     1381 gacaacacgc ccgcctttgg gctggccgcg cagagcaacg cggcttggtc cgcggcgctc
     1441 gacgcgatgc tgacgccgct cggaaagcaa gtgcgcgagg agaacaagcc cgagaacgat
     1501 ccgaaattct tgaactgcca accgtacggc ttcgtccgcg aggtgacgaa tccgctgccg
     1561 atgcagatca gccgcgacgg caagcacctg ctcgtgcgct acgaggaatg gtcgctgcat
     1621 aggccgatct acatggacgg ccgcgcacag ccgacgtacg cgacgccgag cttgttgggc
     1681 catgcggtag gccgcgtcga gaacggcgcg ctgatcgtcg agacgacgcg cgtcacgccc
     1741 gacttgatct cggacaactc gcaggtcggc catagcggcg agctcgaggc cgtcgagcgt
     1801 tacgtcgtgc gcgacaatcc gcgccggctc gagctcacgt tgacgctcac ggatcccgtg
     1861 acgttcacga agccgctcgt cgttacgaag acctggcttt acacgcccga cgtggagctc
     1921 gtgcaggaca cctgcaacca acagcccggc aagccctgat tccgctcgtt agtcggcgga
     1981 gctccgccga gtcagtggga ggcagcgcaa tgaaatcggc agccgcgttc ctgctcatcg
     2041 gcgcctcggc gttgccggcc gctacggcaa cggcgcaaaa caccacgtcg gaaggcggct
     2101 acgaagtcac gagatcgcag accgtcgccg acgcgccggc cggttacgtc ggccgcaaga
     2161 cgacggatcg cgagacgcgc atcggcaaca cgcccgagac cgacggcaac tcggcgacgt
     2221 tcgtcatgac ggtcggaggc ttcgttcgcg aatgcccgag cgcggaaggc atcgcggcgg
     2281 gcaacttcga gtactcgctg acggccgacg acgtgaacac cgacgaaggc gagacgaggc
     2341 gcacgcattc tctcgtcagc ctaatcgcaa gtctcgaagg gcacgtgcgc gacgacggca
     2401 tcgtcgacta cgtcgtcgtc gacggcgatt tcacgcggca acgcgaaggt tcgccggccg
     2461 agcagcagca cgtgcaacgg cgcttcaccg tcggcgcgca agggcaaccc gatttcgcgg
     2521 caatggaaga cgccgtctct gcgacggcag acgtctcgat cgcgatcgtg atgtggatgg
     2581 gcagcacgct ctacacgcag gcgcaaacgc actggaacat gctcgacgct tgcgccgagc
     2641 tcgcgttcga gccggcgacg cggacgcgcg cgctgggacc gaatcagtcg gctgacgtcc
     2701 gtgtcacgtt gcggaccaag gccgacaaag cgccgatcgg cggcgcggaa ttccaggcgg
     2761 ggccgctcga gggaatcggc gcgttgacgc cgcgcggcgg gcgcaccgag agcgcgccga
     2821 tcgatttcac ctatacggcg tcggccaatc cgaagaacgg gaatggcttc gacatcggtg
     2881 caaggtcgcg cgcgggtctc gcttcgggca aatggcagat cgtcgcggcc acgccgctcg
     2941 aaggaacgtt cacgcagacg cgcacgatga acatgagcgg cgccacgttg ccgcccgagg
     3001 tgcgcgcgct cggcgcggaa cggtacggca tcggcacgtc ctcgaactac gagatcacgg
     3061 ggcgcctgcg ctggaccgta gaggaaggct cgacacgacc atcgagcttc ggcgacgccg
     3121 ggtccacgtt ctacgtgccc accgacggtg agatcacggt aaaggtccgc agcgacggcc
     3181 gctccgtcgc cggcgtgtgc accatcgaag gcggccgcac gttccggctc gccgagctgc
     3241 cgcgcgaagc
//
LOCUS       DASSNN010000543         3298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1225024, whole genome shotgun sequence.
ACCESSION   DASSNN010000543 DASSNN010000000
VERSION     DASSNN010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225024"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFL84_10140"
     CDS             complement(<1..295)
                     /locus_tag="VFL84_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609026.1"
                     /translation="MREPKSTGLPPAQGLYDPKFERDSCGVGFVAHIKGKRSHKIIED
                     ADHLLCRMDHRGARGAEPNTGDGAGMLTALPHEFLAKAAKQAFGATLPPPGRFA"
     gene            complement(613..1311)
                     /gene="pdsR"
                     /locus_tag="VFL84_10145"
     CDS             complement(613..1311)
                     /gene="pdsR"
                     /locus_tag="VFL84_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HET7609027.1"
                     /translation="MPDAKLVAVVEDEAAIRDNYLAGLRRHGYRVVGFASRKDALAGF
                     ANRFPDLVIIDVNLGTEVEGGFELCRELRAQSAQLPIIFLTARESELDAVSGLRLGAD
                     DYLTKRTSIEHLLARVAALFRRVEALRAGDAAAERLEQGSLTLDRDRMSVQWKGTPVP
                     LTVTEFWVVHALAQHPGHVRSREQLMTAANVVLDDGTVTSQIKRIRAKFLGVDAKFDA
                     IQTVYGMGYRWHAD"
     gene            1440..2123
                     /locus_tag="VFL84_10150"
     CDS             1440..2123
                     /locus_tag="VFL84_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018144657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7609028.1"
                     /translation="MRIQNWSKVAALMLGCALTLPACTTLDPYSREERPARAQRQAII
                     GAAVGGAVGLITGDSSVERKKRALVGAGLGALAGAGVGAYMDRQEARLRAELERTGVS
                     VTRNGDNITLNMPGNITFAFGSADLNASFYDVLNSVSMVLAEFDQTVIEIAGHTDSVG
                     TEAANQALSERRAASVASYLGTRNVRTDRIITVGAGETRPIATNDTDAGRQANRRVEL
                     TLVPLTLES"
     gene            complement(2284..3147)
                     /gene="rpoH"
                     /locus_tag="VFL84_10155"
     CDS             complement(2284..3147)
                     /gene="rpoH"
                     /locus_tag="VFL84_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HET7609029.1"
                     /translation="MTSTALATRPAELMLAGPLGSLDHYIQAANAIPVLTAEEEFALA
                     TRLRDTNDLDAARRLVLSHLRFVIHVARGYAGYGLQLGDLIQEGNVGLMKAVRRFDPN
                     VGVRLVSFAVHWIRAEIHEFVLRNWRLVRVATTKAQRKLFFNLRKAKKHLGWLSKEET
                     EAVAADLGVTPRDVTEMEKRLSGHDLAFDPYPEEDDDFAPSTYLPSPNSDPAQAVERD
                     DAGANEHERLTAALAKLDERSRVILSRRWLAEKKATLHELAAEYGVSAERVRQIEANA
                     IGKLKKQLVAA"
BASE COUNT          529 a         1080 c         1111 g          578 t
ORIGIN      
        1 ccgcgaatct gcccggcggc ggcaacgtgg cgccgaaggc ctgcttcgcg gctttcgcta
       61 ggaactcgtg cggcagcgcc gtcagcattc ccgcgccgtc gcccgtgttg ggctcggcgc
      121 cgcgggcacc gcggtggtcc atgcggcaca gcaggtgatc cgcgtcctcg atgatcttgt
      181 ggctgcgctt gcctttgatg tgcgcgacga aaccgacgcc gcagctgtcg cgctcgaact
      241 tggggtcgta caacccttgt gccggcggaa ggccggtgct ctttggttca cgcatgcttg
      301 ccgtcctcat gccgcaagag cgttcacgac gcatcggccg gtacgcgccc gtttgggcgc
      361 gccgcgtcga tgccgtttcg tgtgtgtcgc tcttgcagtc atggtactaa cagcggtcag
      421 ccgtctagct ccgctgagtc gagtttttct tgaggcaggc agttttgcga cgccgacctg
      481 tcaagccgcc ggcccattcg atctgccgcg gagtggcccc aaattgagga ccggagcagg
      541 ccttgtgcca acgccagact atagggaacg cctctgcgcc gcgtcaatcg cgacgtcgcg
      601 cgaggacaac agtcagtcgg cgtgccagcg gtagcccatt ccgtagacgg tttggatcgc
      661 gtcgaacttc gcgtcgacgc ccaagaactt ggcgcgaatg cgtttgatct gcgaggttac
      721 cgtgccgtcg tcgagcacga cgttggccgc cgtcatcagc tgctcgcggc tgcgcacgtg
      781 gccgggatgc tgcgcgagcg cgtgcacgac ccagaactcc gtgacggtga gcggcacggg
      841 cgtgcctttc cactgcacgc tcatgcgatc gcgatcgagc gtcagcgatc cttgctcgag
      901 ccgctcggcc gcagcgtccc cggcgcgcag cgcctcgact cggcggaaca gcgccgcaac
      961 gcgcgcgagc aggtgctcga tgctcgtgcg cttggtcaag tagtcgtcgg cgccgagcct
     1021 aaggcccgag acggcgtcga gctcgctctc gcgggccgtc aggaagatga tcggcagctg
     1081 cgccgactgc gcccgcagct cgcggcagag ctcgaagccg ccctcgacct cggttccgag
     1141 attgacgtct atgatgacga gatcggggaa tcggttcgcg aacccggcga gcgcgtcttt
     1201 gcggctggca aagcccacga cgcgatagcc gtgccgccgc aggcccgcca ggtagttgtc
     1261 acggatggcg gcctcgtcct cgacgacggc tacgagcttg gcgtcaggca tggcctaaag
     1321 gcaggcgcgg caggcggcgc ccgggcccgc gctttgcata ttcctcgatt ggagctaggt
     1381 cacgtttcgt tcaggttcgg cttggcatga taccgcccgt cacatcagga ggaaccggta
     1441 tgcggattca gaactggtct aaggtcgctg ctctgatgct cggctgcgcg ctgacgctgc
     1501 cggcgtgcac gacgctcgac ccctatagcc gcgaagagcg accggcacgc gcgcaacggc
     1561 aagcgatcat tggagcggcc gttggtggcg ccgtgggcct catcacgggt gacagctccg
     1621 tcgagcgcaa gaagcgcgcg ctcgtcggcg caggtctagg agccttggcc ggcgcgggtg
     1681 tcggtgccta tatggaccgg caggaagcga ggctgcgcgc cgagctcgag cggaccggcg
     1741 tcagcgtgac ccgcaacggc gacaacatca cgttgaacat gcccggcaac atcacatttg
     1801 cgttcggcag cgccgatctg aacgcgagct tctacgatgt gttgaattcc gtcagcatgg
     1861 tgctggccga gttcgaccag accgtcatcg agatcgcagg tcatacggac agcgtcggca
     1921 cggaggcggc caaccaggcg ctgtccgagc ggcgggcagc ctcggtcgcc tcgtacctcg
     1981 gcacgcgcaa cgtccgcacg gaccgcatca tcacggtcgg cgcgggtgag acgcgcccga
     2041 tcgcgacgaa cgacacggac gcgggccggc aggcgaaccg gcgcgtcgag ctcacgctgg
     2101 tcccgctgac cctggaaagc tgagaggtct cctagacgac gaagggcgca ccgtctccgg
     2161 tgcgcccttc ttcattgggg cctatcgaaa aggggacgga tttatttaat ccgcgccctg
     2221 tttcttgcgc ggaccaatca tcaatgtgcc ggggcggatt aaaaaaatcc gtccccgcgc
     2281 gaatcaggcc gcgacgagtt gcttcttgag cttgccgatc gcgttggcct cgatctgccg
     2341 cacgcgctcg gccgaaacgc cgtactcggc ggcgagctcg tgcaacgtgg ccttcttctc
     2401 ggcgagccag cggcgcgaca ggatcacgcg gctgcgctcg tcgagcttcg ccagggccgc
     2461 cgtcagccgc tcgtgctcgt tggccccggc atcgtcgcgc tcgacggcct gcgcggggtc
     2521 gctgttcggg ctcggcaaat aggtcgaggg cgcgaagtcg tcgtcctctt cggggtaggg
     2581 gtcgaacgcg aggtcgtggc cgctcagccg cttctccatc tcggtcacgt cgcgcggcgt
     2641 gacgccgagg tcggccgcga cggcctcggt ctcctccttg ctgagccagc cgagatgctt
     2701 cttggcctta cgcaggttga agaacagctt ccgttgcgcc ttcgtcgtcg cgacgcggac
     2761 gagccgccag ttgcgcagca cgaattcgtg gatttcggcc cggatccagt ggaccgcgaa
     2821 cgagacgagg cgaaccccga cgttggggtc gaaccggcgc acggccttca tgaggccgac
     2881 gttgccttcc tggatcaagt cgccgagctg caggccgtag cccgcatagc cccgcgccac
     2941 gtgaatcacg aaacgcaggt gtgacaggac gagacgccgc gcggcgtcga ggtcgttcgt
     3001 atcgcgcaag cgcgtggcga gcgcgaactc ttcctccgcg gtcaacaccg ggatggcatt
     3061 ggcggcctga atgtagtggt ccaggctgcc gagcgggccc gcgagcatca actccgcagg
     3121 ccgggtagcc aaagcagtcg aagtcatggt tcctgtacct cccgaagcgc gcatgttagc
     3181 actcgtcgcc ttagagtgct aataaccgga tcggtttcgg gggcctattt cggctctatg
     3241 gcgcgtaaat ggcgggcggc ggccaggccg gcgccggccc agcccaagaa ccccccac
//
LOCUS       DASSNN010000544         4191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1841585, whole genome shotgun sequence.
ACCESSION   DASSNN010000544 DASSNN010000000
VERSION     DASSNN010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841585"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFL84_10160"
     CDS             <1..516
                     /locus_tag="VFL84_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609030.1"
                     /translation="VPPEFADRDREVLNGQGVRVGEEEGEITEAERAVQAEREAQAEA
                     DRKAKEEIARRDRMLLETYISVADIEDLRNRRLELLESQIKLTEVYLGNLRKRLVDLQ
                     AEAGNYKPYSTREDAPQIPDNLKLDISRTTASIAQYEQTLSRTRTNQQELQASFDDDI
                     TRFRQIKGVGY"
     gene            complement(521..988)
                     /gene="dut"
                     /locus_tag="VFL84_10165"
     CDS             complement(521..988)
                     /gene="dut"
                     /locus_tag="VFL84_10165"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET7609031.1"
                     /translation="MTTTARRIELKILNARIGKDIPLPKRATPGSAGLDLRACLDAPL
                     ELAPGETKLIPTGLAIHIADPQLAAVIIPRSGLGHKHGIVLGNLVGLIDSDYQGELMV
                     SCWNRGSAAFRIEPGERIAQLVVVPVVQAEFAVVENFAASDRGSGGFGHTGRS"
     gene            complement(1015..2262)
                     /gene="coaBC"
                     /locus_tag="VFL84_10170"
     CDS             complement(1015..2262)
                     /gene="coaBC"
                     /locus_tag="VFL84_10170"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET7609032.1"
                     /translation="MATTERVPAPRVLLGVTGGIAAYKAPEIVRRLVESGAEVQVVMS
                     RGASEFVGPLTFQAVSGRRVRDDLWDKEAEAAMGHIELARWADVVVVAPATANFLGTL
                     AAGLAGDLLSTVCLATTAPLVVAPAMNQAMWANAAVQANRAVLESRGVRFLGPATGDQ
                     ACGETGPGRMLEPNEIAAAVLEQLDPTRARPLKGLKVVVTAGPTREPIDPVRYITNRS
                     SGKMGFAVAAAARDAGAEVVLVTGPVALPTPASVRRVDVETAEEMYKKVHDEIGGAHI
                     FVACAAVSDYRPREAAERKIKRSAAEMELALVRSPDTLASVAELPRAPFTVGFAAETH
                     DVALHAREKLERKRIDMIAANQVGRDCGFDRETNALTVFWPGGELALGEAGKVQLARR
                     LVEIIAERYRSSRAIKKPSSRAG"
     gene            2417..3088
                     /gene="radC"
                     /locus_tag="VFL84_10175"
     CDS             2417..3088
                     /gene="radC"
                     /locus_tag="VFL84_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET7609033.1"
                     /translation="MKSGWPLSERPRERLLEAGPKALSDGELLAILLGPGARGRSAAE
                     LARGLLAERGGLVALLGSTPAELERASGLGPARVARLAAALELGRRYLGAPLEPRSAL
                     GAPEDAARFLKARLLDLPHEVFCCLFLDTRHRLIRYEELFRGTIDGATVYPREVVKRA
                     LAHNASAVILGHNHPSGVSEPSEADRSITLKLAKALALVEVRLLDHVVVSRGGHVSLA
                     ERGLV"
     gene            3163..3399
                     /gene="rpmB"
                     /locus_tag="VFL84_10180"
     CDS             3163..3399
                     /gene="rpmB"
                     /locus_tag="VFL84_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET7609034.1"
                     /translation="MSRVCQVTGKGPMVGNKVSHANNKTKRRFLPNLHTQRFWLENEK
                     RFVRLRVSRQGLRIIDKKGIEQVVAELRAQGQHI"
     gene            3431..3586
                     /gene="rpmG"
                     /locus_tag="VFL84_10185"
     CDS             3431..3586
                     /gene="rpmG"
                     /locus_tag="VFL84_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET7609035.1"
                     /translation="MREKIRLMSSAGTGHYYTTTKNRRLHPEKMEVLKYDPVVRKHVT
                     YKETKIK"
     gene            complement(3720..>4191)
                     /locus_tag="VFL84_10190"
     CDS             complement(3720..>4191)
                     /locus_tag="VFL84_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7609036.1"
                     /translation="KIRRVAELPKLEESSRTAAELDKLRAIMVHRMHVLRHYTLNVTL
                     PVLRRELESLGENANSLLRATRRHLSWAPEMLDDSARHRLNELAQRHPRFKTVLQFRS
                     ELKQLWEGAHTSNERLLADFHAWCARAEQSGIQGLQEFVAYLKSFRAMHEPALA"
BASE COUNT          706 a         1411 c         1400 g          674 t
ORIGIN      
        1 gtgccgcccg agttcgcgga ccgagaccgc gaggtcctga acggtcaagg cgtgcgcgtc
       61 ggcgaggaag aaggcgagat caccgaagcc gagcgtgcgg tccaggccga gcgtgaagcc
      121 caagccgaag cggatcgtaa ggcgaaggaa gagatcgcgc gccgcgatcg catgctgctc
      181 gagacgtata tctcggtcgc cgacatcgag gatctgcgca atcgccggct cgagctgctc
      241 gagtcgcaga tcaagctcac cgaggtttac ctcggcaact tgcgcaagcg gctcgtcgac
      301 ctgcaagccg aggccggcaa ctacaagccc tactccacgc gcgaggatgc gcctcagatt
      361 cccgacaacc tgaagctcga catctcgcgg acgactgcgt cgatcgctca atacgagcag
      421 acgctgtcgc gcacacgcac caaccagcaa gagctgcaag cgtcgttcga cgacgacatc
      481 acccgcttcc gccagatcaa gggcgtcggc tactagcgtt tcagctgcgg cccgtgtggc
      541 cgaatccgcc gctgccgcga tcgctcgcgg cgaaattttc cacgaccgcg aattccgcct
      601 gcacgactgg cacgacgacg agctgcgcga tgcgctcgcc gggctcgatg cggaatgccg
      661 cggagccgcg gttccagcac gagaccatga gctcgccttg atagtcggaa tcgatgagcc
      721 cgacgagatt gccgagcacg atgccgtgct tatggccgag ccccgagcgc gggatgatga
      781 cggccgcgag ctgcggatcg gcgatgtgaa tcgcgagacc cgtgggtatc agcttcgtct
      841 cgccgggcgc tagctcgagc ggcgcatcga gacaggcgcg caggtcgaga ccggcggagc
      901 ccggcgtcgc tcgcttcggt agcggaatgt ccttgccgat gcgcgcgttg aggatcttga
      961 gctcgatacg ccgggcagta gtcgtcacga acggggcctc gaggatcggc gccttcagcc
     1021 ggcgcgagag gaaggtttct tgatcgcgcg ggaagaacgg tagcgctcgg caatgatctc
     1081 gacgagccgg cgcgccagct ggactttgcc cgcttcgccg agcgcgagct cgccgccggg
     1141 ccagaacacc gtcaacgcat tcgtctcgcg gtcgaacccg cagtcgcggc cgacctggtt
     1201 cgcggcgatc atgtcgatgc gcttgcgctc gagcttctct cgcgcgtgca gtgccacgtc
     1261 gtgtgtctcg gcggcgaagc cgaccgtgaa cggcgcgcgc ggcagctccg cgacgctcgc
     1321 gagcgtatcg ggcgagcgca ccaaagcgag ctccatctcc gcagcggagc gcttgatctt
     1381 tctctcggcc gcttcgcgcg ggcgatagtc cgagaccgcc gcgcacgcga cgaagatgtg
     1441 cgcgccgccg atctcgtcgt gcaccttctt gtacatctct tccgccgttt cgacgtcgac
     1501 gcgccgcacg gacgcgggcg tcggcagggc gacgggcccc gtcacgagaa cgacttcggc
     1561 gcccgcgtcg cgcgccgccg cagcaacggc gaagcccatc tttccggagc tgcgattcgt
     1621 gatgtagcgc accgggtcga tcggctctcg cgtcgggccg gccgtgacga cgaccttgag
     1681 ccctttgagc gggcgagcac gcgtcggatc caactgctca agcaccgccg cggcaatctc
     1741 gttcggctcg agcatgcggc ccggtcccgt ctcgccgcac gcttgatcgc ccgtggcggg
     1801 cccgagaaac cgtacgccgc gcgattccag tactgcgcga ttcgcctgca cggcggcgtt
     1861 cgcccacatc gcttgattca tcgcgggcgc gacgacgagc ggagccgtgg tcgcgaggca
     1921 gacggtcgag agcagatctc cggccagccc cgccgcgagc gtgccgagaa aattggccgt
     1981 ggccggggcg acgacgacga cgtcggccca gcgcgcgagc tcgatgtggc ccatcgcggc
     2041 ttcggcttcc ttgtcccaga ggtcgtcgcg gacgcgccgg ccggaaaccg cctggaacgt
     2101 caggggcccg acgaactcgc tcgcgccgcg tgacatcacg acctgaactt cagcgccgct
     2161 ctcgacgagg cggcggacga tctcgggcgc tttgtacgcc gcgattccgc cggtcacgcc
     2221 gagcaacact cgcggtgcgg ggacgcgttc ggtggttgcc atgggcattg ctccggggcg
     2281 gagcttactc tcggttcttc cggccatgcc actggctaat cgagcttttc ttatgtcttc
     2341 ggggactaat cgctgctgcc gcgcccgcgc gggcacgcga cgatcagcaa ccatcgtttg
     2401 gtcgccggag aaccccatga agagcggctg gcccctctcg gaacggcctc gcgagcgcct
     2461 gctcgaggcg gggcccaaag cgctctccga cggagagctg ctcgcaatcc tgctcggccc
     2521 cggcgcgcgc ggccggtcgg ccgccgagct cgcgcgcggg ctgctcgccg agcgcggcgg
     2581 tctcgtcgcg ctgctcggca gcacgccggc cgagctcgag cgagcgagcg gcttggggcc
     2641 ggcgcgcgtc gcgcgtctcg cggcggcgct cgagctcggc cggcgttatc tcggggcgcc
     2701 gctcgagccg cgcagcgcgc ttggcgcgcc cgaggacgcg gcgcggttcc tgaaggcacg
     2761 gctgctcgat ctgccgcacg aggtgttctg ttgtctcttc ctcgacacgc gccaccgctt
     2821 gatccgctat gaggagctat tccgcggcac gatcgacggc gcgacggtct atccccgcga
     2881 ggtcgtcaag cgagcgctgg ctcacaacgc gtccgcggtg atcctggggc acaatcatcc
     2941 ctccggtgtg tcggagccga gcgaggccga tcgcagcatc acgctcaagc tcgcgaaggc
     3001 attggcgctc gtcgaggtca ggctcctcga ccacgtcgtc gtgagccgcg gcggccacgt
     3061 ctcgctggcc gagcgcggcc tcgtctaggc cccgattctt gcggccgcag ggggtcagcg
     3121 ggtatagtcc ccgccctttc gacgcctgct tagggattag ccatgtccag ggtctgccaa
     3181 gtcaccggca aagggccgat ggtcgggaac aaggtttccc acgcgaacaa caagacgaag
     3241 cgccgtttct tgccgaatct gcacacgcag cgtttctggc tcgagaacga gaagcgcttc
     3301 gtgcggctgc gcgtctcgcg ccaggggctt aggatcatcg acaagaaggg catcgagcag
     3361 gtcgtcgccg agctgcgcgc ccaaggccag cacatctaag agagcgccag aacctgtaga
     3421 gagatggacc atgcgtgaaa aaattcgatt gatgtcctcg gccggcaccg gccactacta
     3481 cacgacgacg aagaatcgcc gcctgcatcc cgagaagatg gaggtcctca agtacgaccc
     3541 cgtcgtgcgc aagcacgtca cgtacaagga aacgaagatc aagtagccgc gagcgctacg
     3601 gtcgaaaaaa acccgccttt cggcgggttt tttattggct gcggctcaaa aaggggacag
     3661 tccccaatac gttcgcccca aaaaggggac agtccccatt acgcccggcg cggcgacgct
     3721 tacgcgagcg ccggctcgtg catcgcgcgg aacgacttga ggtacgcgac gaactcctga
     3781 aggccttgaa tgccactctg ctcggcgcgc gcgcaccagg catggaagtc ggcgagcagg
     3841 cgctcgttgc tcgtgtgcgc gccttcccag agctgcttca gctcgctgcg aaactgcagc
     3901 acggtcttga atcgcggatg acgctgcgcg agctcgttca aacggtggcg cgcggaatcg
     3961 tcgagcattt cgggcgccca gctcaggtgc cgccgcgtgg cgcgcagcaa agagttcgca
     4021 ttctcgccta ggctctcgag ctcacggcgc agcaccggca gcgtgacgtt cagcgtgtag
     4081 tggcgcagca cgtgcatgcg gtggaccatg atcgctcgca gcttgtcgag ctcggccgcc
     4141 gtgcgcgacg actcctcgag cttcggcagc tcggcgacgc gacggatctt c
//
LOCUS       DASSNN010000545         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1841608, whole genome shotgun sequence.
ACCESSION   DASSNN010000545 DASSNN010000000
VERSION     DASSNN010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841608"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VFL84_10195"
     CDS             <1..1012
                     /locus_tag="VFL84_10195"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7609037.1"
                     /translation="VTEVAAAWPGFLGGIGGAPAYGEGATGKQIVRYQFVSPEYFGVL
                     GIDVVRGRGFSAGERNPSEAVAVVSETVARELWPGADALGQVLRLEPNPSIVRAGAIS
                     SAAAAAQASGDPLYQPRTAVVIGVARDVAGFTIGGVRFGGAGIYMPIDTDAATTALTL
                     RVRGDADVASRAILDRFAALDPNMAQVSTMETLTQIDTYILGSTFWVTIVLGALALLL
                     TLSGLFSVLSYLVEQRTREIGVRMALGASSSSIGALVLKESIWPVGIGLVVGCTLTVG
                     FCALLLATPAAEQIGAIVQLFDPVAYGASIAFIVAACAGAALLPALRAGGVNPLVALR
                     QE"
     gene            complement(1102..1854)
                     /locus_tag="VFL84_10200"
     CDS             complement(1102..1854)
                     /locus_tag="VFL84_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7609038.1"
                     /translation="MITNSESGTRIDEIAEGIYRINTPLALPGEVGFSFNQYLIVDDA
                     PMLFHSGMRRLFPLVSEAIRAVLPLERLRYVGLSHFEADECGALNQFLAAAPNAQAVC
                     GQIAALVSVNDYADRAPRALADGEQLSLGRHVIRWFETPHVPHAWECGLMLDTTTRTF
                     FCGDLFTQGGAGDVALTEADILGPSEAFRKPMDYFAHTPSTRAQLERLAREAPTTLAC
                     MHGSAWRGDGAALLRELAAVLERSQPAARAAA"
     gene            2100..>2560
                     /locus_tag="VFL84_10205"
     CDS             2100..>2560
                     /locus_tag="VFL84_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609039.1"
                     /translation="MSSSSAQSALAAPGRPPRLNLENQRKQARSLLKAVRAGDAQALR
                     RLAPLSPKRPHSAVAADRQHWSLHHAQLVIAREQGFTSWAKLKAHIEADSATLRLALV
                     GAAHRALETDVDEPLFADPLARALAGKRGLALHKELRSTTWPPHAAGPAPE"
BASE COUNT          361 a          891 c          908 g          400 t
ORIGIN      
        1 cgtgaccgag gttgcggccg cgtggcccgg tttcctggga ggcatcggcg gcgcgcccgc
       61 gtacggcgag ggcgccaccg gcaagcagat cgtccgttac cagttcgtgt cgcccgagta
      121 cttcggcgtg ctcggcatcg acgtcgtgcg cggccgcggc ttctcggccg gcgagcgcaa
      181 tccgagcgaa gccgtggccg tcgtgtccga gaccgtcgcg cgtgagcttt ggcccggcgc
      241 cgacgcgctc gggcaggtgc tgcgcttgga gcccaacccg tcgatcgtgc gcgcgggcgc
      301 catctcgtcc gcggcggccg cggcgcaagc ctccggcgat ccgctctacc agccgcgcac
      361 ggccgtcgtg atcggcgtcg cgcgcgacgt cgcgggcttc acgatcggcg gtgtcaggtt
      421 cggcggcgcc ggcatctaca tgccgatcga caccgacgcg gccacgacgg cgctgacgct
      481 gcgcgtgcgc ggcgacgccg acgtcgcgag ccgcgcgatc ctcgatcgat tcgcggcgct
      541 cgatccgaac atggcgcagg tctccacgat ggagacgctg acgcagatcg acacgtacat
      601 tctcggcagc acgttctggg tcacgatcgt gctcggcgcg ctggccctgc tgctgacgct
      661 gtcggggcta ttcagcgtgc tgtcgtatct cgtcgagcag cgcacgcgcg agatcggcgt
      721 gcgcatggca ctcggcgcca gcagctcgag catcggcgcg ctcgtgctga aggaatcgat
      781 ttggccggtc ggcatcgggt tggtcgttgg gtgcacgctg actgtcggct tctgcgcgtt
      841 gctgctcgcg acgccggcgg cggagcagat cggcgcgatc gtgcagctgt tcgacccggt
      901 cgcgtacggc gcgagcatcg cgttcatcgt ggcggcgtgc gcgggggccg cgctgcttcc
      961 ggcgctgcgc gcgggcggcg tcaatccgct cgtcgcgctg cggcaagagt gacagcaccc
     1021 ttgcgcggtc ggattcttgg gccatcggca tgcgccgccg cattgctcgc cgccgcggcg
     1081 cgcagccgct gaactgcggg attaggcggc ggcgcgcgcc gccggctgcg agcgctcgag
     1141 cacggccgcg agctcgcgca gcagcgccgc gccgtcgccg cgccaggcgc tgccgtgcat
     1201 gcaggcgagc gtcgtcggcg cctcgcgtgc gagccgctcg agctgcgcgc gcgtcgacgg
     1261 cgtgtgcgcg aagtagtcca tcggcttacg gaaagcttcg ctcggcccca agatgtcggc
     1321 ttccgtcagc gcgacgtcgc cggcgccgcc ttgcgtgaac aagtcgccgc agaagaacgt
     1381 gcgcgtcgtc gtgtcgagca tgaggccgca ttcccacgcg tgcggcacgt gcggcgtctc
     1441 gaaccatcgg atgacgtgcc ggccgagcga cagctgctcg ccgtccgcga gcgcgcgcgg
     1501 cgcgcggtcg gcgtagtcgt tgacggagac gagcgccgcg atttggccgc aaaccgcttg
     1561 cgcgttcggt gcggccgcga ggaactgatt cagcgcgccg cactcgtcgg cctcgaagtg
     1621 cgagagcccg acgtagcgca accgctcgag cggcagcacg gcgcggatcg cctcgctgac
     1681 caacggaaac aaccgccgca tgccgctgtg gaacagcatc ggcgcgtcgt cgacgatcaa
     1741 gtactgattg aagctgaagc cgacctcgcc cggcagcgcg agcggcgtgt tgatgcggta
     1801 gatgccctcg gcgatctcgt cgatgcgcgt gccggactcc gaattcgtga tcatgagcgg
     1861 tctcccctgg ttgaaagcct gaaatcgcaa gggagtggca cgacgccgcg cgtcgttaca
     1921 gcggtcggcg gcgaatgctt gacgccgccg cgcccgttgc aaaatacttc gcgtcagcgc
     1981 gcagcagagc gcgccccgtc cgcggtaagg gccacgccca tctgtcgctc gacacctttc
     2041 gtatgtgacc tcttttcagc ctgacgagcg gacgacaggc cacttgaaag gaggtttcca
     2101 tgtcatcgtc atcggcgcaa tccgctttag cagcacccgg gcgcccgccg cgcctcaacc
     2161 tcgagaacca acgcaagcaa gcccgctcgt tgttgaaggc cgtgcgcgcc ggcgacgcgc
     2221 aggcgttgcg ccggctcgcg ccgttgagcc cgaagcgccc ccattccgcc gtcgccgcgg
     2281 atcggcagca ctggtcgctg caccacgctc agctcgtgat tgcgcgcgag cagggattta
     2341 cgagctgggc caagctcaaa gcgcacatcg aagccgacag cgcaacgttg cggctcgcgc
     2401 tcgtcggggc cgcgcatcgc gcgctcgaga ccgacgtcga tgagccgttg ttcgccgatc
     2461 cgctcgcgcg cgcgctcgcc ggcaagcgag ggctcgcgtt gcacaaggag ctgcgctcga
     2521 cgacgtggcc gccgcacgcg gcgggaccgg ctcccgagca
//
LOCUS       DASSNN010000546         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1225262, whole genome shotgun sequence.
ACCESSION   DASSNN010000546 DASSNN010000000
VERSION     DASSNN010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225262"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFL84_10210"
     CDS             <1..334
                     /locus_tag="VFL84_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017270651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bicarbonate-binding protein"
                     /protein_id="HET7609040.1"
                     /translation="YGNGKIVESSPHVMKFWRDHASYPYRSHELWFLVVGVRWGRLAS
                     SDDLGDLVAKVNREDLWREAAEELGVPNAEIPRSTSRGVETFFDGKVFDPANPQAYLA
                     SLAIKRSS"
     gene            395..1111
                     /gene="ntrB"
                     /locus_tag="VFL84_10215"
                     /pseudo
     CDS             395..1111
                     /gene="ntrB"
                     /locus_tag="VFL84_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017270650.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
     gene            1119..1919
                     /locus_tag="VFL84_10220"
     CDS             1119..1919
                     /locus_tag="VFL84_10220"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7609041.1"
                     /translation="MESNERYVHIENVGKSFGGSSGTEVLRDVSLTVRKGELVSIIGH
                     SGCGKSTLLNVVAGLTRATSGNVFVDNREVVEPGPDRAVVFQNHSLLPWLSVYQNVRL
                     AVDKIFGGVMTSSERHEWTLRNLELVSMAEARGKKPAEISGGMKQRVGIARALSMKPK
                     LLLLDEPFGALDALTRAHLQDRVSEISMELGNTVMMITHDVEEAVLLSDRIVMLTNGP
                     AATIGSICEVPLERPRLRLALISDPTYVECRAAVLEFLHKRHRFVDAA"
BASE COUNT          317 a          687 c          703 g          380 t
ORIGIN      
        1 ctacggcaac ggcaagatcg tcgagtcgag cccgcacgtc atgaagttct ggcgcgacca
       61 cgcgtcgtat ccgtaccgaa gccacgagct ctggttcctc gtcgtgggcg tgcgctgggg
      121 caggctcgcg tcgagcgatg acttgggtga cctcgtcgcg aaggtcaatc gcgaggactt
      181 gtggcgcgaa gccgccgagg agctcggcgt gccgaacgcg gagataccgc ggtcgacgtc
      241 gcgcggcgtc gagacgttct tcgacggcaa ggtcttcgac cctgccaatc cgcaggcgta
      301 cctcgcgagc ctcgccatca agcgctcgag ctgaccgatg agcttgcgga acgcggcgcc
      361 gccgctcgtc gccgtcggcg cgatcgtcgt cctttgggag ctcctgtgcg cggctcccga
      421 cgcgagcttg ccgccgccgt cgcgcgtcgt cggcgagacg tgggagctca tcgcgcaccc
      481 gttctacgac cgcggcggca tcgacaaagg ccttttctgg cacatctacg cgagcttgaa
      541 gcgcgtcgcg ctcggctatc tgctcgcgtc gattgccggc atcgcgctcg gcgtgctcgt
      601 cggcagcagc cggctcgcga cgcgcgcact cgacccgatc ttccaggtgc tgcgcacgat
      661 tccgccgctc gcgtggctgc cgctgtcgct cgcggcattc cgcgacggcc agccgtccgc
      721 gatcttcgtg atcttcatca ccgcgatctg gccgatcatc atcaatacgg ccgtcggcat
      781 ccggaacatc ccgcaggatt accagaacgt cgcgaaggtc ttgaggctca atcgcggcga
      841 atacttcgtg tcgatcatgt tgccggcggc cgcgccgttc atcttcacgg gcttacgcat
      901 cggcgtcggc ctgtcgtggc tcgcgatcgt cgcgggcgag atgctgatcg gcggcgtcgg
      961 tatcggcttc ttcatctggg actcgtggaa cagctcgcgg ttgagcgaca tcatcgtcgc
     1021 gcttgcctac gtcggcgccg tcggcttgct gctcgaccgc gcgatcgccg tgctcgggcg
     1081 catcgtcacg cgcggcacgg cgagcacctg agacgcccat ggaaagcaac gagcgctatg
     1141 tgcacatcga gaacgtcggc aagagcttcg gcggctcgag tggcacggag gtgctgcgcg
     1201 acgtgtcgct gacggtgcgc aagggcgagc tcgtctcgat catcggccac tcgggctgcg
     1261 gcaagtcgac gctgctcaac gtcgtcgcgg ggctgacgcg cgcgacctcg ggcaacgtct
     1321 tcgtcgacaa tcgcgaggtc gtcgagcccg gtcccgatcg cgccgtcgtg tttcagaacc
     1381 actcgctgtt gccgtggctc agcgtctatc agaacgtgcg gctcgccgtc gacaagatct
     1441 tcggcggcgt gatgacgagc tccgagcggc acgagtggac gctgcgcaac ctcgagctcg
     1501 tcagcatggc cgaggcgcgc ggcaagaagc ccgccgagat ctcgggcggg atgaagcagc
     1561 gcgtcggcat cgcgcgcgcg ctgtcgatga agccgaagct tctgctgctc gacgagccgt
     1621 tcggcgcgct cgacgcgctg acgcgcgcgc atctgcaaga ccgcgtgagt gagatcagca
     1681 tggagctcgg caacacggtc atgatgatca cgcacgacgt ggaggaggcc gtgctgctct
     1741 ccgatcgcat cgtcatgctg acgaacggcc ccgcggcgac gatcggctcg atctgcgagg
     1801 tgccgctcga acggccgcgc ttgaggttgg cgctgatctc ggatcccact tatgtagagt
     1861 gtcgcgcagc cgtgctcgag ttcctgcaca agcgtcaccg gttcgtcgac gcggcgtagc
     1921 gcgttcgttg ctgccgctac agacggtagc ggatgaggta gctgccgagc tccttggctc
     1981 gctgccacag cgagcgcgtg cgccactctt cgagcttgat ctcgacggcg ttgttttcat
     2041 cggcggcgaa gacgtcctcg agctgctgcg cgaagtgcgc gccgaga
//
LOCUS       DASSNN010000547         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1608033, whole genome shotgun sequence.
ACCESSION   DASSNN010000547 DASSNN010000000
VERSION     DASSNN010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608033"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFL84_10225"
     CDS             complement(<1..400)
                     /locus_tag="VFL84_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609042.1"
                     /translation="MGGRRRPSAWLSSDLRQQLADEAARLMAEHGIQDFSLAKRKAAE
                     RLGVRSSESALPSNAQIQERLVERQRIFEPDVHDRRIAKLRLVATNVMGVLDLFRPKL
                     VGPVLDGTATVNSPIELHVFSDSPEAVAAAL"
     gene            complement(405..>2896)
                     /locus_tag="VFL84_10230"
     CDS             complement(405..>2896)
                     /locus_tag="VFL84_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET7609043.1"
                     /translation="IFLTILALAVGFALVMSGLFIGAYYYLAPGLPQAAELRDIKVQV
                     PLQVYSRDGRLIAEFGEQKRTPVSYDRIPPLLINAVVAAEDEHFFEHPGVDWHGVVRA
                     GLAFLTGRDVGGSTITQQVPRTLDVLKRAGLRSGFDRFVAKYKEMILAYRMEQEFTKE
                     EILELYLNTTFFGQRAYGVATAAQTYFGKNLDALSVSEIAILAGIPQRPAEWNPIYST
                     ENAAMRRAYVLRRMTETGAIDDAQYKAALDEHIVAQRFGTQSQLEARYVAEMVRAEMV
                     ARFGNAATTAGLKVTTTIDSRLQAASNRAMHANLIAYDERHGYRGPLAQLALPAGAVN
                     EADPLAPTDVDAEALRKLLEDYPTLLDYESAIVLGSDDVSARVFFADHGAESIGLDAV
                     EWAGAFVNDDVKGANPTTVAEVLKAGDVVRFRRTAEGGWRLAQLPEAQGAFVALDPFD
                     GAIVALNGGLDFFLNNFNRATQARRQPGSSFKPFVYSAAFENGFNPASVVLDAPVDVG
                     YQPELERVWRPENFNGKYFGPTRLREALLESMNSVTIRMTQGVGVPTVRSHVKRFGFD
                     DEAAPNDLSIALGAGGASPLSIAAGYATFANGGYKVTPYFIDRVVDANGEVVYETEPL
                     YCPECNTPPETPVQLEPEKSELVADAVELYPQQRAAPRIISAQNAYLVGDLMWDNARR
                     GTGARAKRELERNDLAGKTGTTNDGRDLWFVGFNANIVGASWVGFDQFRPLGAYEQGA
                     SAALPMWIGFMREALAGTAEARPKRPRGIVEYRINPKTGKIADDGTPNAIFEKFDIDH
                     IPEREESLISGSFDVSDSGAETEPVKDIFLQ"
BASE COUNT          474 a          966 c          959 g          497 t
ORIGIN      
        1 gaagcgccgc ggcgaccgct tccggagagt cgctgaacac gtgcagctcg atcggcgagt
       61 tcacggtcgc cgttccgtcg agcacggggc cgacgagctt cggccggaat aggtcgagca
      121 cccccatgac gttcgtcgcg acgaggcgca gcttcgcgat tcggcgatcg tgcacgtcgg
      181 gctcgaagat gcgttgccgc tcgaccagcc gctcttggat ctgtgcgttg ctcggcaacg
      241 cgctttcgct cgagcgcacg ccgaggcgct cggcggcctt cctctttgcc aacgagaagt
      301 cttggatgcc gtgctcggcc atcaggcggg ccgcttcgtc ggcgagctgc tgacgcaggt
      361 ccgacgagag ccacgccgaa ggtcgacgac gcccgcccat tgcgctactg gaggaaaatg
      421 tccttgacgg gctccgtctc ggcaccggag tccgaaacat cgaacgaccc gctgatcaga
      481 ctctcctcac gctccgggat atggtcgatg tcgaatttct cgaaaatcgc gttcggcgtg
      541 ccgtcgtccg cgatcttccc cgtcttcggg ttgatgcggt attcgacgat gcctcgcggc
      601 cgcttcggac gtgcctccgc ggtgcccgcg agcgcctcgc gcatgaagcc gatccacatc
      661 ggcaacgcag ccgaggcgcc ttgctcgtag gcgccgagcg gccgaaattg gtcgaatccg
      721 acccacgacg cgccgacgat gttcgcgttg aagccgacga accagaggtc gcggccgtcg
      781 ttcgtcgtgc ccgtcttgcc cgcgaggtcg ttgcgctcga gctcgcgctt tgcgcgcgca
      841 ccggtgccgc ggcgcgcgtt gtcccacatc aggtcgccga ccaggtacgc gttctgggcc
      901 gagatgattc ggggcgcagc gcgctgttgc ggatagagct cgacggcatc ggcgacgagc
      961 tcggattttt ccggctcgag ctgcaccggc gtctcgggcg gcgtgttgca ctcggggcag
     1021 tagagcggct ccgtctcgta gacgacctcg ccgttcgcat cgacgacgcg atcgatgaag
     1081 tacggcgtga ctttgtagcc gccgttcgcg aacgtcgcgt agcccgccgc gatgctgagc
     1141 ggcgacgctc caccggcgcc gagcgcgatc gataaatcgt tcggcgcggc ctcgtcgtcg
     1201 aagccgaagc gcttgacgtg gctgcggacg gtcggaacgc cgacgccctg cgtcatgcgg
     1261 atcgtcacgg agttcatcga ttcgagcagc gcctcgcgca gccgcgtcgg accgaagtac
     1321 ttgccgttga agttctcggg ccgccagacg cgctcgagct ccggctgata gccgacgtcg
     1381 acgggcgcgt cgagcacgac gctcgcgggg ttgaagccgt tctcgaacgc ggccgagtac
     1441 acgaacggct tgaacgagga gccgggctgc cgccgcgcct gcgtcgctcg attgaagttg
     1501 ttcaagaaaa agtcgaggcc gccgttcagc gcgacgatcg cgccgtcgaa cggatcgagc
     1561 gcgacgaacg cgccctgcgc ttcgggaagc tgcgcgagcc gccacccgcc ctcggccgtg
     1621 cggcggaagc gcacgacgtc gcccgccttc aagacctcgg ccaccgtcgt cgggttcgcg
     1681 cccttcacgt cgtcgttgac gaaggcaccg gcccattcga cggcgtcgag gccgatcgac
     1741 tccgcgccat gatccgcgaa gaacacgcgc gcgctgacgt catcggagcc gagcacgatc
     1801 gcgctctcgt agtcgagcag cgtcgggtag tcctcgagca acttgcgcag cgcctcggcg
     1861 tcgacgtcgg tgggcgcgag cggatccgcc tcgttcacgg ctcctgcggg caacgcgagc
     1921 tgcgcgagcg gcccgcgata gccgtggcgc tcgtcgtagg cgatcaggtt cgcgtgcatc
     1981 gcacgattcg aagcggcctg cagccggctg tcgatcgtcg tcgtgacttt gaggcccgcc
     2041 gtcgtcgccg cgttgccgaa acgcgcgacc atctcggcgc gaaccatctc ggccacgtac
     2101 cgcgcctcga gctgcgactg cgttccgaac cgctgcgcga cgatgtgctc gtcgagcgcg
     2161 gctttgtact gcgcgtcgtc gatcgcgccg gtctcggtca tgcgccgcag cacataggcg
     2221 cgccgcatcg ccgcgttctc ggtgctgtaa atcgggttcc actcggcggg gcgctgcgga
     2281 atgcccgcga gaatcgcgat ctcggaaacg gagagcgcgt ccaagttctt gccgaagtag
     2341 gtctgcgcgg ccgtcgcgac gccgtacgca cgctgaccga agaacgtcgt attcaaatag
     2401 agctcgagga tctcctcctt cgtgaattcc tgctccatgc gataggcgag gatcatctcc
     2461 ttgtacttcg cgacgaagcg gtcgaagccg gatctcagtc cggctcgctt caggacatcg
     2521 agggtccgcg gcacttgctg cgtgatcgtg ctgccgccga cgtctctacc ggtcaaaaaa
     2581 gcgagaccgg cgcgaacaac gccgtgccaa tcgaccccgg gatgctcgaa gaaatgctcg
     2641 tcctcggcgg ccacgacggc attgatcagc agcggcggaa tgcggtcgta ggagaccgga
     2701 gtgcgcttct gctcgccgaa ctcggcgatg agccggccgt cgcggctata gacctggagc
     2761 ggcacctgga ctttgatgtc gcgaagctcg gccgcctgcg gcaaacccgg cgcgagatag
     2821 tagtaagcgc cgatgaaaag ccccgacatg accagcgcga aacccaccgc cagggccaaa
     2881 attgtcagga aaatct
//
LOCUS       DASSNN010000548         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_328358, whole genome shotgun sequence.
ACCESSION   DASSNN010000548 DASSNN010000000
VERSION     DASSNN010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328358"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..>2508
                     /gene="uvrA"
                     /locus_tag="VFL84_10235"
     CDS             73..>2508
                     /gene="uvrA"
                     /locus_tag="VFL84_10235"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET7609044.1"
                     /translation="MSDALIVRGAREHNLKNIDVTLPRNALVVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGLMDKPDVDAIEGLSPAISIEQKTAGANPRSTVGTI
                     TEIYDYLRLLWARTGTPHCPNDGTPVERQSASQITDLVLEWPEGTKIEVLAPLVRGRK
                     GEFRDVFEAMRKQGFVRARINGETYDLSDVPKLNRRQNHDIAVVVDRLVVRPADRGRL
                     ADSIETALKAAEGVVEVVRQTGSPRSRLFSERYACPKCGLSLPELEPRQFSFNSPFGA
                     CPDCSGLGTRREVNAELVLGDAGLSILEGVILPWGEPSGYLRKVVLPALAKAYKFDLN
                     APWRDLSPAVQKVMLRGAPGKTFTYQYDSERWHGTYESGWKGVLDHVDKRYRETTSDA
                     VREQLEQYMVEQPCPTCGGKRLKPESLSVLVNGRSIGDVVALPVREALSFFSTLPLRA
                     PGKPGLDPEIAKPILKEVNDRLRFLADVGLEYLTLDRSADSLSGGEAQRIRLATQIGS
                     RLVGVLYILDEPSIGLHQRDNGRLLATLKELRDLGNTVLVVEHDEETIRAADYVVDLG
                     PRAGRFGGEVVAAGALDDVIRHPDSLTSRYLRGELRIPVPPRRRIVSADRMLRVVGAR
                     HHNLKNLTAEFPLGTFIAITGVSGSGKSTLITDILYQALARHFYRAKVVPGAHERIEG
                     LEQIDKVIDIDQSPIGRTPRSNPATYTGLFTPIRELFTYLPESKLRGYGPGRFSFNVK
                     GGRCEACQGDGLVKVEMHFLPDVYVPCEVCKGRRYNRETLEVRYKGKSIADVLDLTVS
                     DALDFFEHQPRIRT"
BASE COUNT          421 a          853 c          826 g          408 t
ORIGIN      
        1 gggcatgggg ctgctaacgt aggaaagcgg agagttgacc gcaagccatt gagctatcta
       61 agctcccggt ctatgtccga cgcgttgatc gtgcgcgggg cacgcgagca caatctcaaa
      121 aacatcgacg tcacgctgcc gcggaacgcg ctcgtggtga tcaccggatt gtccgggtcg
      181 ggcaagtcgt cgctggcgtt cgacacgatc tacgccgaag gacagcgccg gtacgtcgaa
      241 tcgctctccg cctacgcgcg gcagttcctc ggcctgatgg acaagcccga cgtcgacgcg
      301 atcgaaggcc tctcgcccgc gatctccatc gagcagaaga ccgccggcgc gaacccgcgg
      361 tcgaccgtcg gcacgatcac cgagatctac gactacctgc gcctgctctg ggcgcgcacg
      421 ggcacgccgc actgtcccaa cgacggcacg cccgtcgagc ggcagagcgc gtcgcagatc
      481 accgatctcg tgctcgagtg gccggaaggc acgaagatcg aagtgctcgc gccgctggtg
      541 cggggacgga aaggtgagtt ccgcgacgtg ttcgaggcga tgcgcaagca gggtttcgtg
      601 cgcgcgcgca tcaacggcga gacgtacgac ctctccgacg ttcccaagct caatcgccgg
      661 cagaatcacg acatcgccgt cgtcgtcgat cgcctcgtgg tgcgcccggc cgatcgcggg
      721 cgcctcgccg attcgatcga gaccgcgctc aaggccgcgg agggcgtcgt cgaagtcgtc
      781 cgccagacgg gctcgccgcg ttcccggctc ttctcggagc gctacgcctg tcccaagtgc
      841 gggctgtccc tgccagagct cgagccgcgc caattctcgt tcaactcgcc gttcggcgcg
      901 tgccccgatt gctcgggact cggcacccgc cgtgaggtga atgccgagct cgtgctcggg
      961 gatgccgggc tgtcgatcct cgagggcgtg atcctgccgt ggggcgagcc gagcggctat
     1021 ctgcggaaag tcgtgctgcc cgcgctcgcc aaagcgtaca agttcgatct caatgcgccg
     1081 tggcgggatc tgtcgccggc cgtgcagaag gtcatgctgc gcggcgcgcc cggcaaaacc
     1141 ttcacgtacc agtatgacag cgagcgctgg cacggcacgt acgagtcggg ctggaaaggc
     1201 gtgctcgacc acgtcgacaa gcgctatcgc gagaccactt ccgacgcggt gcgcgagcag
     1261 ctcgagcagt acatggtcga gcaaccctgt cccacctgcg gcggcaagcg cctgaagccc
     1321 gagagcctgt cggtcctcgt gaacggccgc agcatcggcg acgtcgtggc gctgcccgtg
     1381 cgcgaggccc tttccttctt tagtacgctg ccgctgcgcg cgcccgggaa gcccggactc
     1441 gatccggaga tcgcgaagcc gatcctcaag gaagtgaacg accggctgcg cttcctcgcc
     1501 gacgtcggcc tcgagtattt gacgctcgat cgctcggccg actcgctgtc aggcggcgaa
     1561 gcgcagcgca tcaggctggc gacgcagatc ggcagccggc tcgtcggcgt gctgtacatc
     1621 ctggacgagc cgtccatcgg gttgcatcag cgcgacaacg gccgcctgct cgcgaccctc
     1681 aaggagctgc gcgacctcgg caacaccgtg ctcgtcgtcg agcatgacga agaaacgatc
     1741 cgcgcggccg attacgtcgt cgatctcggg ccgcgcgccg gacgctttgg cggcgaggtc
     1801 gtggccgcgg gcgcgctcga cgacgtcatc cggcatccgg actcgctcac gtcgcgctac
     1861 ctgcgcggcg agctgcgcat tcccgtgccg ccgcggcgcc gcatcgtctc cgccgaccgc
     1921 atgctgcggg tggtcggcgc gcgccaccac aacctcaaga acctcaccgc cgagtttccg
     1981 ctcggcacgt tcatcgcgat cacgggcgtc tcggggtcgg ggaagtccac gctgatcacg
     2041 gacatcttgt atcaggcgct ggcgcgtcac ttctatcgcg ccaaggtcgt ccccggcgcc
     2101 cacgagcgga tcgaaggact cgagcagatc gacaaggtca tcgacatcga ccagagtccc
     2161 atcggccgga cgccgcgatc gaatcccgcc acgtacacgg gtctcttcac gccgattcgc
     2221 gagctgttca cgtatctgcc ggagtccaag ctgcgcggct acggcccggg gcgattctcg
     2281 ttcaacgtga agggcgggcg ctgcgaggcg tgtcagggcg acggcctcgt gaaagtcgag
     2341 atgcacttcc tgccggacgt ctacgtgccg tgcgaggtct gcaaggggcg gcgctacaac
     2401 cgggagacgc tcgaggtccg ctacaaagga aagtcgattg ccgacgttct ggacctgacc
     2461 gtctcggacg cgctcgactt tttcgagcac cagccgcgta tcaggacg
//
LOCUS       DASSNN010000549         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_472112, whole genome shotgun sequence.
ACCESSION   DASSNN010000549 DASSNN010000000
VERSION     DASSNN010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472112"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VFL84_10240"
     CDS             complement(<1..843)
                     /locus_tag="VFL84_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7609045.1"
                     /translation="MLRFLSLFAAALAFAGTAAAQTITFGASVQLTGPVANTGRYYKD
                     AYEFAIEKVNAAGGVKVGSQQQKIALKLLDNQSDVNLSVRQYTQLLTQDKVNFLLGPF
                     ASNFVLADSSIAEKASVPMVQGGGASDEIYTRGYKYIFGTLPAASDYFGSTVEALGKL
                     NPPPKNVALLFADDSFDVSVAKGTREILKKAGLQTVLDERYSTNASDFSVLLSRIKSS
                     NVDAVLVAGHETEVLNFIRQAKSLNVNPKFYSFTVGVPSADFRHALGKDANGVSGMTA
                     WLPSA"
     gene            1107..2111
                     /gene="ligD"
                     /locus_tag="VFL84_10245"
     CDS             1107..2111
                     /gene="ligD"
                     /locus_tag="VFL84_10245"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET7609046.1"
                     /translation="MVPKAAAAVARDRTPRRRPPVPAMTQIPPDVDDVSIAVDGKDVQ
                     LTNLRKPFWPALGLTKGDLLRYYAAVAPSLLPHVAARAMVMKRYPNGADGDSFFMKRA
                     PRPRPDWIATCSIEHGSGSVIAFPVVDDLASLLWLVNLGCIDLNPWYARCDDVDRPDY
                     LHFDLDPVPDASFDDVRACALIVRDELTSIGLPTYVKTTGSKGVHVYVPIERGPRQKE
                     VWAFAKGIAFSLAKRYPQQMTAEYRIAKRPAGRVLVDYNQNAWGRTLASVYSPRPKPL
                     ATVSTPITWDELAAGASIEDFRIDNVPSRIAALGDLWAPVDAAGGRFDLATLLRVSSR
                     "
     gene            2108..>2246
                     /locus_tag="VFL84_10250"
     CDS             2108..>2246
                     /locus_tag="VFL84_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET7609047.1"
                     /translation="MSLPLAVDYPPAEAKLVDALPQGCEWEYEPKWDGFRCLAFKDGD
                     DV"
BASE COUNT          366 a          752 c          740 g          388 t
ORIGIN      
        1 cgccgacggc agccacgccg tcatgccgct cactccgttc gcgtccttgc cgagcgcatg
       61 acggaagtcg gcgctcggca cgccgacggt gaacgaatag aacttcgggt tgacgttgag
      121 gctcttcgcc tggcggatga aattgaggac ctcggtctcg tgaccggcga cgagaaccgc
      181 gtcgacgttg ctgctcttga tccgcgacag cagaaccgag aagtccgatg cgttcgtgct
      241 gtagcgctcg tcgaggaccg tctgcaaccc cgccttcttc aggatctcgc gcgtcccctt
      301 cgcgaccgac acgtcgaacg agtcgtcggc aaagagcagc gcgacgttct tcggcggcgg
      361 attcagcttg ccgagcgcct ccacggtgct gccgaagtag tcgcttgcag ccgggagcgt
      421 gccgaagatg tacttgtagc cgcgcgtgta aatctcgtcg gaggctccgc cgccctgcac
      481 catcggcacg ctggccttct cggcgatcga cgaatcggcg aggacgaagt tgctcgcgaa
      541 cgggcccagc aggaagttga ccttgtcctg cgtgagcagc tgcgtgtact ggcgcaccga
      601 caggttgacg tccgactggt tgtcgaggag cttcagcgcg atcttctgct gctgcgagcc
      661 gaccttgacg ccaccggccg cgttgacctt ctcgatcgcg aactcgtagg cgtccttgta
      721 atagcgcccg gtgttggcga ccgggccggt caactggacc gaggcgccga aggtaatggt
      781 ctgggcggcc gcggtgccgg cgaaggcgag cgccgcggcg aagagcgaga gaaagcgaag
      841 catggcgaac tccggttggt gtgggtcgat tctcgttgtt gtgctcgtct ccgctcgctt
      901 cgccgccggc gggcgcgaag aagcccctgc acgttcaaag cccggccgcg ccgggggccg
      961 cgaggcgccc ttttgcaggt ccgagcaatt tcttcgattg gagcaggtaa agcttgcatc
     1021 ggttcatccg gaaacggcca aacgcgcacg agaacacagg gcacgccgct tgctgctcat
     1081 ccggattcac cggcctcttc cccaccatgg tacccaaagc cgcggcggca gtcgcgcgtg
     1141 accgaacgcc gcgccgccgg ccaccggttc ccgcgatgac gcagattccg cccgacgtcg
     1201 acgacgtctc catcgccgtc gacggcaagg acgtgcagct caccaatctg cgcaagccgt
     1261 tctggccggc gctcggcctc accaagggcg acctgctgcg ctattacgcg gcggtcgcgc
     1321 cgtcgctgct tccgcacgtc gcggcgcgcg cgatggtgat gaagcgttat ccgaacggcg
     1381 ccgacggcga cagcttcttc atgaagcgtg caccacgacc gcgtcccgac tggatcgcga
     1441 cctgctcgat cgagcacggc tccggcagcg tcatcgcatt cccggtcgtc gacgatctcg
     1501 cgtcgctgct gtggctcgtg aatctcggct gcatcgacct caatccgtgg tacgcgcgct
     1561 gcgacgacgt cgatcgtccc gattacctcc acttcgacct cgatccggtg cccgacgcgt
     1621 cgttcgacga cgtgcgcgcg tgcgcgctga tcgtgcgcga cgagctgacg tcgatcggcc
     1681 tgccgacgta cgtcaagacc acgggatcga agggggtgca cgtctacgtg ccgatcgagc
     1741 gcggcccgcg gcagaaggag gtgtgggctt ttgcgaaggg gatcgcgttc tcgctcgcga
     1801 agcgctatcc gcagcaaatg accgccgagt accgcatcgc caagcgaccc gccggccgcg
     1861 tgctcgtcga ttacaaccag aacgcgtggg gacgcacgct cgcgtcggtc tattcaccgc
     1921 ggccgaagcc actcgcgacg gtgtcgacgc cgatcacgtg ggacgagctc gccgccggcg
     1981 cctcgatcga ggacttccgc atcgacaacg taccgtcgcg catcgccgcg ctcggcgacc
     2041 tctgggcacc ggtggacgcg gcaggcgggc gcttcgacct cgcaacgttg cttcgcgtct
     2101 cgtcgcgatg agcctgccgc tcgcggtcga ctacccgccg gcggaggcga agctggtcga
     2161 cgcgctgccg cagggatgcg aatgggaata cgaacccaag tgggacggtt ttcgctgcct
     2221 cgcgttcaag gacggcgacg acgtgg
//
LOCUS       DASSNN010000550         2668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_328906, whole genome shotgun sequence.
ACCESSION   DASSNN010000550 DASSNN010000000
VERSION     DASSNN010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328906"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFL84_10255"
     CDS             <1..756
                     /locus_tag="VFL84_10255"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HET7609048.1"
                     /translation="LEFEQHPMQREVVGGTMAFPFGKARQLLDFYAEYEASAPDELFL
                     CMGLQNSPEGKAVSFNACYSGPAREAERVFAKVRAAGGTPLRDNLRTIDYVALQRSGD
                     VDDPRAVGSYTKSGFSVGLKPKFIDAILTGFGEQPGGSSVFFLYHCGGAINRVAADAT
                     AFPHREITLTPLLAVSWPIASDPTAQVAWLRKYWASVEPHMQGFYTNDIIDETQKQVD
                     ENYLDNYPRLVALKNKYDPKNLFRLNANVIPSV"
     gene            complement(901..1164)
                     /locus_tag="VFL84_10260"
     CDS             complement(901..1164)
                     /locus_tag="VFL84_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609049.1"
                     /translation="MAIDLDELLALPVEERMKLGEILMDSAVPPDIGPLLRDFVSALE
                     RTTRGLDLAIARLSALDERLARGRAEAREAALSSGETWPFTFS"
     gene            complement(1177..1470)
                     /locus_tag="VFL84_10265"
     CDS             complement(1177..1470)
                     /locus_tag="VFL84_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609050.1"
                     /translation="MPMQAESLEVLEKASVAPAQARAIVRAIEIEVAGAKDTLATKTD
                     LIALAATLRTEIAKQGGDLRAEIAGVIRQMYLALLGQTAVILGFFYFFLTQLR"
     gene            complement(1634..>2668)
                     /locus_tag="VFL84_10270"
     CDS             complement(1634..>2668)
                     /locus_tag="VFL84_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7609051.1"
                     /translation="PELGLVYFSTGNPGPDLNGSVRAGDNLFSVSMVAIEAKTGKYRW
                     HFQQVHHDLWDYDSPNPVVLFDAMYDGRLRKGIVEIGKTGYVYILDRETGKPLVGIDE
                     VPVPQEPRQATAATQPIPRGDEVIPHEIDIEPEGFELINNGRIFTPFWDKPVVVKPLG
                     TGGANWPPSSYDPETNLFYVCTGDGAAAYSTKEGGVEWVIPTPGARYFGGEYTRSRVP
                     RRGVLAAVDMRTNKLAWRQQWGELCYAGSTVTGGGLIFIGRNDGRLSALDKSSGAQLW
                     EFQTDAGIHAAVSTFERNGKQYVVALSAGSFFPNTTRGDSVWLFALDGAGTPSGASPE
                     PSGGAADLTH"
BASE COUNT          475 a          852 c          862 g          479 t
ORIGIN      
        1 ctcgaattcg agcagcaccc gatgcagcgc gaggtcgtcg gcggcacgat ggcattcccg
       61 ttcggcaagg cccgccagct gctcgacttc tacgccgagt acgaggcgag cgcgccggac
      121 gagctgttct tgtgcatggg cttgcaaaat agccccgaag gcaaggccgt ctcgttcaac
      181 gcgtgctaca gcgggccggc gagggaagcg gagcgcgtgt tcgcgaaggt acgcgccgcg
      241 ggcggcacgc cgctgcgcga caacttgcgc acgatcgact acgtcgcgct gcagcgctcg
      301 ggcgacgtcg acgatccgcg cgccgtcggg tcgtacacga agtcgggatt ctcggtgggc
      361 ctcaaaccca agttcatcga cgcgattctg accggcttcg gcgagcaacc gggcggcagc
      421 tcggtattct ttttgtatca ctgcggcggc gcgatcaacc gcgtggccgc cgacgcgacc
      481 gcgttcccgc accgcgagat cacgctgacg ccgctgctcg cggtgagctg gccgatcgcg
      541 agcgacccga ccgcgcaggt cgcgtggctg cgcaagtact gggcgagcgt cgagccgcac
      601 atgcaaggct tctacacgaa tgacatcatc gacgagacgc agaagcaggt cgacgagaat
      661 tatctcgaca actatccgcg gctcgtcgcg ctcaagaaca agtacgatcc gaagaacttg
      721 tttaggttga acgcgaacgt gatcccgagc gtttgatcaa cgtctcgatg tacgtaaggc
      781 gccggtcatg atcgagcaga cggtccccca tcttgtcgac atcgaccgtc aaacgctcga
      841 cgcgttcttg gacgaggatc gcttgccgta tggcactgcg taaatcgaac cagacgcgca
      901 tcacgagaac gtaaacggcc aagtttcacc ggagctgagc gccgcttctc gcgcttctgc
      961 tcgaccacgt gcgagccgct catcgagtgc gctcaaacgt gcgatcgcga gatcgaggcc
     1021 acgggtcgtt cgctcgagcg cgctcacgaa gtctcgtagc aatggaccaa tgtccggcgg
     1081 cacggcgctg tccatcagga tctcgcctaa cttcatgcgc tcctcgacag gcagtgccaa
     1141 tagctcgtcc aagtcgatcg ccattggcgg cccgcgtcag cgcagctgcg tgaggaagaa
     1201 gtaaaagaag ccgagtatga ccgccgtctg gcccaggagc gccaagtaca tttgacgtat
     1261 gacaccggcg atttccgctc gtaggtcgcc gccctgtttc gctatctcgg tacgaagagt
     1321 agccgccaag gcaatcagat cggtcttcgt tgcgagcgtg tccttggcgc cggcgacctc
     1381 gatttcgatg gcgcgcacga tcgcgcgcgc ctgggccggt gccacgcttg ctttctcgag
     1441 aacctcgagc gattccgctt gcatcggcat tcgatgataa gccccgccaa aagcgcagtg
     1501 tcaagcgacg ctggaggaga taatcgctca gcgccctctc ttcgcggagc gccgaaaacc
     1561 gtgaaagcga cgagttactg cgccaaaaag gggacagatc tattttcccg cgaaaataga
     1621 tctgtcccct tttctagtgc gtgaggtctg ctgcgccgcc ggagggctcc gggcttgcgc
     1681 cgcttggggt tccggcgccg tcgagcgcga agagccagac gctgtcgccg cgcgtcgtgt
     1741 tcgggaagaa cgagcccgcc gagagcgcga cgacgtactg cttgccgttg cgctcgaacg
     1801 tgctgacggc ggcgtggatg ccggcgtcgg tctgaaattc ccaaagctgc gcgccgctcg
     1861 atttgtcgag cgcgctcaac cggccgtcgt tgcggccgat gaagatcagg ccgccgcccg
     1921 tgacggtcga gcccgcgtag cagagctcgc cccactgctg gcgccacgcg agcttgttcg
     1981 tgcgcatgtc gacggccgcg agcacgccgc ggcgcggcac gcgcgagcgc gtgtactcgc
     2041 cgccgaagta gcgggcaccg ggagtcggaa tgacccattc gacgccgcct tccttcgtcg
     2101 aatacgccgc ggcgccgtcg cccgtgcaaa cgtagaacag attcgtctcg ggatcgtatg
     2161 agctcggcgg ccagttcgcg ccgcccgtgc cgagcggctt cacgacgacg ggcttgtccc
     2221 agaacggcgt gaagatgcgg ccgttgttga tgagctcgaa tccctcgggc tcgatgtcga
     2281 tctcgtgcgg gatgacctcg tcgccgcgcg ggatcggctg cgtcgcggcc gtcgcttggc
     2341 gcggctcttg cggcaccggt acctcgtcga tgccgacgag cggcttgccc gtctcgcgat
     2401 cgaggatgta gacgtagccc gtcttgccga tctcgacgat gcctttgcgt aggcggccgt
     2461 cgtacatcgc gtcgaacagc acgaccggat tcggcgagtc gtagtcccag aggtcgtgat
     2521 gcacttgctg gaagtgccag cggtatttgc ccgtcttcgc ttcgatcgcg accatcgaga
     2581 cgctgaagag attgtcgccg gcccgcaccg agccgttcag gtccgggccc gggttgcccg
     2641 tcgagaagta gacgagcccg agctcggg
//
LOCUS       DASSNN010000551         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_958397, whole genome shotgun sequence.
ACCESSION   DASSNN010000551 DASSNN010000000
VERSION     DASSNN010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958397"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VFL84_10275"
     CDS             complement(<1..989)
                     /locus_tag="VFL84_10275"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HET7609052.1"
                     /translation="MSRLTRRAATVLVAVLASTAAYAQRQSPANRAAPPPPSTPPPAA
                     ATPNADASTQALLDRIDELERRLSQLESTAVISQPKVLVKEVRVWVDDNGNQYDHQVP
                     GSKEVITYERELAQRRETIADSIDEKLAEEAAGGVEIGINNVTTLQFAHQTKGDDAVA
                     DGHAYGLSAADITFAANSAALNTEFYADLVGIGGTPPEQEIDSLSLINGQTARLSNNQ
                     LSVREAWLRTELAHQKLGLSIGRIDVTTHFDRNAAANDETTQFISDALVNNPVLGLAS
                     NGFGTVAEWDPKKSWNFKFGLQQSTDPNNQLGSLADSMFRLAEFEYIARPFGLP"
     gene            complement(1193..2647)
                     /locus_tag="VFL84_10280"
     CDS             complement(1193..2647)
                     /locus_tag="VFL84_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET7609053.1"
                     /translation="MSFECSRRTLLSAGALVAGAMVLPAARSRAQEIGRVMDELSRYM
                     AAARERALPPEVVEHTKHHVLDTFAAMVSGAKLEPGQAALRYAATQTHGGAATVIGAG
                     FTLDPAAAALVNGTCAHADETDDSHGPSQSHPGAAVVPAALALGESLRLDGTRFLRAV
                     ALGYDVGPRLTMALGGITFRNESRRSTHSFAATFGAAAAAGCAAALDAREMRWLLDYT
                     SQQTAGYAVWSRDTEHIEKAFVFAGMPARAGVTAAELVSAGWTGVDDVFSGEDNFFAV
                     NAPNGDPKLLVDELGMRYEVGNTDIKKWSVGTPIQAPLDALELTRKQRPFAAADVSEV
                     VVRLAPTVGAVVDNRDIPDICLQHMLAVMLLDGTASFAAAHDAARMRDAAVLRERAKV
                     RYVPDAELAALLPARVAVVEVVLTDGTRLTERVEAVRGTVRNPMGRDEVVTKARDLIE
                     PVLGGAIAGRLIATILALEETRDLRSLRRLLQRR"
BASE COUNT          412 a          941 c          963 g          488 t
ORIGIN      
        1 ggcaggccga acggccgcgc gatgtactcg aactcggcga gcctgaacat cgaatcggcg
       61 agcgagccga gctggttgtt cggatccgtg ctctgctgca ggccgaactt gaagttccag
      121 ctcttcttcg ggtcccattc cgcgaccgtg ccgaagccgt tgctggcgag acctagcacc
      181 gggttgttca cgagcgcgtc gctgatgaat tgcgtcgtct cgtcgttcgc ggcggcgtta
      241 cggtcgaagt gcgtggtcac gtcgatgcgg ccgatgctca agccgagctt ttggtgcgcg
      301 agctcggtgc gcagccacgc ttcgcgcacg ctcaactggt tgttgctcaa gcgcgccgtc
      361 tggccgttga tgaggctgag cgagtcgatc tcttgctcgg gcggcgtgcc gccgatgccg
      421 accaagtcgg cgtagaactc cgtgttcagc gcggccgagt tcgcggcgaa cgtgatgtcg
      481 gcggccgaga ggccgtaggc atggccgtcg gccaccgcgt cgtcgccctt cgtctggtgc
      541 gcgaattgca gcgtcgtgac gttgttgatg ccgatctcga cgccgcccgc cgcttcctcg
      601 gcgagcttct cgtcgatcga atccgcgatc gtctcgcggc gctgcgcgag ctcgcgctcg
      661 tacgtaatca cctctttcga gccggggacc tgatggtcgt actggttgcc gttgtcgtcg
      721 acccagacgc ggacttcctt cacgagcacc ttcggctgcg agatgaccgc ggtcgactcg
      781 agctggctca ggcgccgctc gagctcgtcg atccggtcga gcagcgcctg cgtgctcgcg
      841 tccgcattcg gcgtcgcggc ggccggcggc ggggtcgagg gcggcggcgg agcggcacgg
      901 ttcgccggcg actggcgttg tgcgtaagcg gccgtgctcg ccagcacggc gacgagcacc
      961 gtcgccgccc ggcgcgtgag ccgagacacc gaaaagttta agtgcagcat ggcctttccc
     1021 ccgagctcct gacgaaaaca gtgggcgcga aaacacgacg cggcgcagac cgccgacggg
     1081 tcatgatcga gtacgcctcg gggtttgtcc cgatatttac ggttgaacaa accgttaaag
     1141 gacgctaagc ggtgccgtta atggaagccg ctgaagggcc cccgcgcttc ccttaccgcc
     1201 gttgcaggag ccgccgtagc gaacggaggt cccgggtctc ctcgagcgcg aggatcgtcg
     1261 cgatcaagcg gcccgcgatc gccccgccga gcaccggctc gatgaggtcg cgtgccttcg
     1321 tcacgacctc gtccctgccc atcgggttcc tgacggtccc gcgcacggcc tcgacgcgct
     1381 ccgtgaggcg cgtaccgtcc gtcaagacga cctcgacgac ggccacgcgc gcgggcagca
     1441 gcgccgcgag ctccgcgtcc ggcacatagc ggacttttgc gcgctcgcga agcactgccg
     1501 catcgcgcat ccgtgcggca tcgtgcgcgg cggcaaacga tgccgtgccg tcgagcagca
     1561 tgaccgcgag catgtgctgc aggcagatgt cgggaatgtc gcggttgtcg acgacggcgc
     1621 cgaccgtcgg cgccagacgc acgacgacct cgctcacgtc tgccgctgcg aacggccgct
     1681 gtttgcgcgt gagctcgagg gcgtcgagcg gcgcctgaat cggcgtgccg acgctccact
     1741 tcttgatgtc cgtgttgccg acttcgtagc gcataccgag ctcgtcgacg agcaacttgg
     1801 ggtctccatt gggcgcgttg acggcgaaga agttgtcctc accggaaaag acgtcgtcga
     1861 cgccggtcca gccggcgctg acgagctccg ccgcggtcac gcctgcccgc gccggcatgc
     1921 cggcgaacac gaatgctttt tcgatgtgct ccgtgtcgcg gctccacacc gcgtagccgg
     1981 ccgtttgttg cgacgtgtag tcgaggagcc aacgcatctc gcgcgcgtcg agagcggctg
     2041 cgcagccggc agcggccgcc gcgccgaacg tcgcggcgaa cgaatgcgtg ctgcggcgcg
     2101 actcgttgcg gaacgttatg ccgccgagcg ccatcgtgag ccgcgggccg acgtcgtagc
     2161 cgagcgcaac ggcgcgcaag aaccgcgtgc cgtcgaggcg caagctctcg ccgagcgcga
     2221 gtgccgccgg cacgacggcg gcgcccgggt gcgattgcga cggcccgtgc gaatcgtccg
     2281 tttcatctgc gtgcgcgcac gtaccgttga cgagcgcggc ggcggcgggg tcgagggtaa
     2341 agccggcacc gatcaccgtc gctgcaccgc cgtgcgtctg tgtggccgcg tagcgcagcg
     2401 cggcctgtcc gggctcgagc ttcgcgccgg agaccatcgc ggcgaaagta tcgagcacgt
     2461 ggtgcttggt gtgctcgacg acctccggcg gcaacgcccg ctcgcgcgca gccgccatgt
     2521 agcggctgag ctcgtccatg actctgccga tttcctgcgc gcggctgcgc gcggccggca
     2581 gcaccatcgc gcccgcaacg agcgctcctg cgctgagcag cgtgcggcgt gagcattcga
     2641 acgacatgat tccccttctc gcggccggct ggagtttact ccgtacactg gccgtacacc
     2701 gaacgcatgt ttatgcttcc tacgactcgc gattttgggg ggagtcgaag aatgaaccga
     2761 aacctgctgt ggctggccgt gccgtttctc gcagcgtgcg cgcc
//
LOCUS       DASSNN010000552         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1608669, whole genome shotgun sequence.
ACCESSION   DASSNN010000552 DASSNN010000000
VERSION     DASSNN010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608669"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VFL84_10285"
     CDS             <1..623
                     /locus_tag="VFL84_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7609054.1"
                     /translation="QSVPLYDAGHDLVCAISDYFVHDDSTCYELGTSTGELLRKLAMH
                     HSKPNVRWIGIDPVEPMVAKAREHCAGVPNIEIVCEDAIAHDYEKADLFISYYCVQFV
                     QPRHRQELLNRVYERLHWGGAFILFEKVRGPDARFQDMSVALYNDFKAKNGFSADEIL
                     NKTTSLKGVLEPFSTEGNLGLLRRAGFTDIMSVMKWVCFEGFLAVK"
     gene            721..>2320
                     /locus_tag="VFL84_10290"
     CDS             721..>2320
                     /locus_tag="VFL84_10290"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HET7609055.1"
                     /translation="MDTQARFRSTRAVPWLAPRLWLAFGVLALGAAIAALRPAAAQEN
                     LAGAEELRELVGPIALYPDDLVAIVLPASTYPLQIVQAARFLEDRKRDSSLKPDEDWD
                     DSVVALLNYPEVIKLLNDDLDWTYDLGTAVLNQREDVLSAIQDFRDEAYAAGNLRSDE
                     RQIVAREDDAIEIKPANPQVIYVPYYEPEQVVVYQPAPVYYYYPVAYPVYYYPYPAHH
                     HFHTGFFWGVNTWFSIGWHSHYLHVYDPFYYGHPYYGWTYYDPFYVRNVYVNVNHYHY
                     PNYVWEPRYRDGGRPVTRSSEGRVYTADRDRTPHRGALDSSTTRTREGSVDRARGGAA
                     SGAQPSTDTAQANRTRTTPNGRSTRAETPPGQTHGQTHQPVRQARESGQVQAREPGGS
                     TDGSAAQPSRTRSGGGMSQALERSRAPQANADARTGSGAARSNPSMARANPGVSPRAS
                     ERSTGMPQQSSRSAPPRYQQAAPQREAPPPRQQQSFDSSSRGGGTSAQRSQPPSGGGQ
                     PGNSGGNGGGSYRGNSGGGNSGGGH"
BASE COUNT          422 a          785 c          747 g          366 t
ORIGIN      
        1 gccaatccgt gccgctgtac gacgccgggc acgatctcgt ctgtgcgatc agcgactact
       61 tcgtgcacga tgacagcacc tgctacgagc tcggcacctc gaccggagag ttgctacgga
      121 agctcgcgat gcaccattcg aagccgaacg tccgctggat cggaatcgat ccggtcgagc
      181 cgatggttgc aaaggcgcgc gagcactgcg ccggcgtccc gaacatcgag atcgtctgcg
      241 aggacgccat cgcgcacgac tacgagaagg ccgatctctt catctcgtat tactgcgtcc
      301 agttcgtgca gccgcgacac cggcaagagt tgctgaacag agtttacgag cggctccact
      361 ggggcggcgc cttcatcctg ttcgagaagg tgcgcgggcc ggacgcgcgc ttccaagaca
      421 tgagcgtcgc gctgtacaac gatttcaagg cgaagaacgg tttcagcgcc gacgagatcc
      481 tcaacaagac gacgagcctg aagggtgtac tcgagccctt ctcgaccgaa ggcaatctcg
      541 gcctgctccg ccgcgccggg tttacggaca tcatgtcggt gatgaaatgg gtctgcttcg
      601 agggcttcct cgcggtcaag tagcgtcatt aaggagccgt tcagctggcc caggctaggc
      661 tccagccatc gtgggtgtag accgtttgcc ggtcgcactc attagttagg agacctcacg
      721 atggacacgc aagcccgctt ccgctcgacc cgtgcggtgc cgtggctcgc tccgcggctc
      781 tggctcgcgt tcggcgttct cgcgctcggt gcagcgatcg cggcgctgcg cccggccgca
      841 gcgcaagaga acctcgccgg tgccgaggag ctgcgcgagc tcgtcggccc cattgcgctc
      901 taccccgacg acctcgtcgc gattgtcctc cccgcgtcga cgtatccgtt gcaaatcgtc
      961 caggccgcga ggttcttgga agatcgcaag cgcgactcga gcttgaagcc ggacgaggac
     1021 tgggacgact ccgtcgtcgc gctgttgaac tatcccgagg tcatcaagct tctgaacgac
     1081 gatctcgact ggacctacga tctcggcacg gccgtgctga accaacgcga ggatgtgctg
     1141 agcgcgatcc aagattttcg cgacgaagcg tacgccgcgg gcaatctacg cagcgacgag
     1201 cggcagattg tcgcgcgcga ggatgacgcg atcgagatca agccggcgaa tccgcaggtc
     1261 atttacgttc cgtactacga gccggagcag gtcgtcgtct atcaacccgc gccggtctac
     1321 tactactacc cggtcgcgta cccggtctat tactacccct atccggcgca tcaccacttc
     1381 cacaccgggt tcttctgggg cgtcaacacg tggttctcga tcggttggca ctcgcactac
     1441 ttgcacgtct acgacccgtt ctactacggc catccgtact acggctggac gtattacgac
     1501 ccgttctacg tgcgcaacgt gtacgtgaac gtcaaccact atcactatcc gaattacgtt
     1561 tgggagccga ggtaccgcga tggcgggcgc ccggtcacgc gcagcagcga gggccgcgtc
     1621 tacacggcgg atcgagatcg cacgccgcat cgcggcgcgc tcgactcgag cacgactcgc
     1681 acgagggaag gctccgtcga tcgcgcacgc ggaggtgccg cgagcggcgc acagccgtcg
     1741 accgacaccg cgcaggcgaa ccggacgcgc accacgccaa acggacggag cacgcgtgcg
     1801 gaaacgcctc ccgggcagac gcacgggcag acgcatcagc ctgtgcggca agcgcgagaa
     1861 tccggtcaag tgcaagcgcg tgagcccggt ggctcgaccg acggctctgc ggcgcagccg
     1921 agccgcacac gcagcggcgg cggaatgtcg caggcgctcg agcggagccg cgcgccgcag
     1981 gcgaatgcgg acgctcgcac gggctccggc gccgcgcgct cgaatccgag catggcgcgc
     2041 gcgaatcccg gcgtctcgcc gcgcgcgagc gagcgctcga cgggcatgcc gcagcaatcg
     2101 tcacgcagtg ccccgccgcg ctatcagcaa gcggcgccgc agcgcgaggc tccgccaccg
     2161 cgccagcagc agtcgttcga ctcgtcgagc cgcggcggcg gaacgagcgc gcagcgcagc
     2221 cagccgccgt ccggcggcgg ccagcccggt aactcgggcg gcaacggcgg cggcagctac
     2281 cgcggcaact caggcggcgg caattccggc ggcgggcacg
//
LOCUS       DASSNN010000553         3733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_225000, whole genome shotgun sequence.
ACCESSION   DASSNN010000553 DASSNN010000000
VERSION     DASSNN010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225000"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..595)
                     /locus_tag="VFL84_10295"
     CDS             complement(134..595)
                     /locus_tag="VFL84_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET7609056.1"
                     /translation="MALTVTRQELTAMVGRELGASDWFLIDQARVNAFADVTLDHQFV
                     HVDVERAKATPFGGTIAHGFLTLSLLVHLCLPFIPEPVNRKLVVNYGFDKVRFSAPVK
                     VGKRIRAVAKLGDVNERRPGNIVIRLDVTVEIEGENKPALVAEWLSLHVVD"
     gene            798..2150
                     /gene="rimO"
                     /locus_tag="VFL84_10300"
     CDS             798..2150
                     /gene="rimO"
                     /locus_tag="VFL84_10300"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET7609057.1"
                     /translation="MQVPKAATKTHSPKVGFVSLGCPKALVDSERILSQLRAEGYETA
                     ADYAGADVVVVNTCGFLDSARAESLEAIGEALAENGRVIVTGCLGAEANVIRDAHPNV
                     LAITGPQQYEAVVTAVHAAIPPPHDPFVDLVPAQGVKLTPRHYAYLKISEGCNHRCSF
                     CIIPRLRGDLVSRPAADVLGEAERLVRAGVRELLVISQDTSAYGVDVKYASSTWRGRA
                     VEARITDLAAALGELGAWVRLHYVYPYPHVDELIPLMAEGKVLPYLDVPFQHASPKIL
                     KAMKRPAAEAKTLQRIREWRAAVPTLTIRSTFIVGFPGETEQDFQALLDWLEEAELDR
                     VGCFRYEPVNNAAANALPNAVPEEVKEERWHRFMAAQQAISARRLVRRVGTDIDVIVD
                     EVDGTQALGRSMADAPEIDGRVYLPAGQGAKKIKAGAIVTARVTSADEYDLWAEPVGQ
                     "
     gene            2483..>3733
                     /locus_tag="VFL84_10305"
     CDS             2483..>3733
                     /locus_tag="VFL84_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET7609058.1"
                     /translation="MNDNSKKNHTASNGLRRWLPGLSVLREYEAAWLPRDIMAGLVLT
                     TVLVPVGIAYATASGVPGIYGLYATIVPLLAYALVGPSRILVLGPDSSLAAVILAVVA
                     PLAAGDPARAVALASGMAVVSGLTCIAIGLLRVGFVTELLSKPIRYGYMNGIALTVLI
                     SQIPKLLGFSVEDGTGPVRDLVGIAQGIGGGRVSWTAAAVGGATLLAILLLKPYKRVP
                     GILIAVLGATIAVGVLNLDTTAGVGVLGPLPQGLPAFALPLISPEHVRDVVVGGFAVA
                     MVAFADTSVLSRTYAAKTRTAVDPNREMIGLGAANLAAGLFQGFPVSSSSSRTPVAEA
                     AGAKTQLTGVVGALAIASVLLAAPNLLQYLPSSALAAVVIAAAIGLFEVADLRRIFRI
                     NNWEFWLSIVCFIGVATFGVIPGIG"
BASE COUNT          594 a         1275 c         1229 g          635 t
ORIGIN      
        1 catctccatc tgcggcgtga tccgcgacgc ggcttgcgcg ggcggcgcag ccgcctgcga
       61 gcccgcgccc gtatcggcgt cgcggccgca agccgcgagc ccaagtgccg caacgacaac
      121 ccactcgatt cgtttaatcg acgacatgta agctcaacca ttcggcgacg agcgccggtt
      181 tgttttcacc ttcgatctcg accgtcacgt cgagccgtat cacgatgttc ccgggcctgc
      241 gctcgttcac gtcgccgagc ttggcgacgg cccggatacg cttgccgacc ttgacgggcg
      301 ccgagaaccg tactttatcg aaaccatagt tcacgacgag cttgcggttc acgggctccg
      361 ggatgaacgg caagcacagg tgcacgagca gcgacagcgt caagaagccg tgcgcgatcg
      421 tgccgccgaa cggcgtcgcc ttcgcgcgct cgacgtcgac gtggacgaat tggtgatcga
      481 gcgtgacgtc cgcgaaagca tttacgcgcg cctgatcgat caaaaaccag tccgacgcgc
      541 cgagctcgcg gccgaccatg gccgtaagct cctgtcgcgt caccgtcagc gccatgcgcg
      601 ccctcctaat ggggacagac cccttttccc taatggggac agaccccttt tcggcaaaaa
      661 accggaagaa aatagatccg tccccttttt gaattgtggt ctgccggcct tgatcaggta
      721 aagccgggtt gccttttgcc gccgtgcgtg gcgcaatagc gccccctgcg cgtcccgccg
      781 caacccccgt ttcgagcctg caagtgccga aagccgctac gaaaacccat tctcccaagg
      841 tcggcttcgt gagcctcggc tgcccgaagg cgctcgtcga ttccgagcgg atcctgagcc
      901 agctgcgcgc cgaggggtac gagacggccg ccgattacgc gggcgccgac gtcgtcgtcg
      961 tgaacacgtg cgggttcctc gacagcgcgc gcgccgagtc gctcgaagcg atcggcgaag
     1021 cgctggccga gaacggccgc gtgatcgtga ccggatgcct cggggccgaa gcgaacgtca
     1081 ttcgcgacgc gcatccgaac gtgctcgcga tcacggggcc gcagcaatac gaagccgtcg
     1141 tcacggccgt gcacgccgcg atcccgccgc cccacgatcc gttcgtcgat ctcgtgccgg
     1201 cgcagggcgt caagctgacg ccgcgccact acgcgtactt gaagatctcc gaaggctgca
     1261 atcaccgctg cagcttctgc atcatcccgc gcttgcgcgg cgatctcgtc agccggcccg
     1321 ccgccgacgt gctcggcgaa gccgagcgcc tcgtgcgcgc gggcgtgcgc gagctgctcg
     1381 tgatctcgca ggacacgagc gcgtacggcg tcgacgtcaa atacgcttcg tcgacgtggc
     1441 gcggccgcgc cgtcgaggcg cgtatcacgg atctcgccgc ggcgctcggc gagctcggcg
     1501 cgtgggtgcg cctgcactac gtatatccgt atccgcacgt cgacgagctg attccgctga
     1561 tggccgaggg caaagtgctg ccgtatctcg acgtgccgtt ccagcacgcg agcccgaaga
     1621 tcttgaaggc gatgaagcgg cccgcggccg aggccaagac cctgcaacgc atccgcgagt
     1681 ggcgtgcggc cgtccccacg ctgacgattc gctcgacgtt catcgtcggc ttcccaggcg
     1741 agacggagca ggattttcag gcgttgctcg actggctcga ggaagccgag ctcgaccggg
     1801 tcggctgctt ccgctacgag ccggtgaaca atgcggccgc gaacgcgctg ccgaacgcgg
     1861 tgccggagga agtcaaagaa gaacgctggc accgcttcat ggccgcgcag caggcgatca
     1921 gcgcgcggcg gctcgtgcgc cgcgtcggca ccgacatcga cgtcatcgtc gacgaggtcg
     1981 acggcacgca ggctctcggc cggtcgatgg ccgatgcgcc cgagatagac gggcgcgtct
     2041 atctccctgc cggccaaggc gcgaagaaaa tcaaagcggg cgcgatcgtc acagcgcgcg
     2101 tcacgagcgc cgacgagtac gatctctggg ccgagccggt cggccagtga ccgagctgcc
     2161 ggtagcgacg gagtgaacct ctcggtgccc tcgcgcgctt aactgagtgg cggcagcgag
     2221 ccacaaccct cgtcgcaagt cacgcgcaag ctgatccatg cgggcatgcc ggcttacctg
     2281 attccgtccc cgtgctgcga caagttcgac tacctctacg acgcgcgggg cgtcgtcttg
     2341 tgcgctcctt ccggcggttt cacggggcgt ggcgacggaa gttgtccgga ggtgttgggg
     2401 acaggcgccg cgcaagatcc ttgaaatgaa cggtgcggct tggtagctag acgctgcagt
     2461 ccgagctccc cgcgccgagt gaatgaacga caactcaaag aagaaccaca ccgcatcgaa
     2521 cggcttgagg cgctggctcc cggggctcag cgtgctgcgc gagtacgagg ccgcatggct
     2581 gccgcgcgac atcatggccg gcctcgtcct gaccacggtg ctcgtgccgg tcggcatcgc
     2641 gtacgcgacc gcttcgggcg tccctggaat ctacgggctc tacgcgacga tcgtgccgct
     2701 gctcgcctac gcgctggtcg gtccgagccg catcctcgtg ctcgggccgg actcgtctct
     2761 ggccgccgtg attctcgctg tcgtcgcgcc gctcgcggcc ggagatccgg ctcgcgcggt
     2821 cgcgctcgcg agcgggatgg ccgtcgtgtc gggactcacg tgcatcgcga tcggactgct
     2881 gcgcgtcggc ttcgtcacgg agctgctgtc taagccgatc cgctacggct acatgaacgg
     2941 catcgcgctg acggtgctca tcagccagat cccgaagctg ctcggcttct cggtcgagga
     3001 cggcaccggc ccggtgcgcg acctcgtcgg catcgcgcaa ggcattggcg gcggccgagt
     3061 gagctggaca gccgcggcgg tcggcggtgc gactctgctc gcgatactct tgctcaagcc
     3121 ttacaagcgc gtcccgggca tcttgatcgc ggtcctcggc gccacgatcg ccgtcggcgt
     3181 gctgaacctc gacacgaccg ccggcgtcgg cgtgctcggc ccactaccgc agggcttgcc
     3241 ggcgttcgct ttgccgctga tcagccccga gcacgttcgc gacgtcgtcg tcggcggctt
     3301 cgccgtcgcg atggtggcgt tcgccgacac cagcgtcttg tcacgcacgt acgccgcgaa
     3361 gacgcgcacg gccgtcgatc cgaatcggga gatgatcggc ctcggcgccg cgaatctcgc
     3421 cgccgggctg ttccaggggt ttccagtcag cagcagctcg tcgcggacgc ccgtcgccga
     3481 agcggccggc gcgaagacgc agctcacggg agtcgtggga gcgctggcca ttgcgtcggt
     3541 gctcctggcc gcgccaaacc tgctccagta tctgccgagc agcgcgctcg ccgctgtcgt
     3601 gattgcggcg gccatcggct tgttcgaggt cgcggacttg cgacgcatct tccgcatcaa
     3661 caactgggag ttctggctgt cgatcgtctg cttcatcggc gtcgcgacgt tcggtgtgat
     3721 tccgggcatc ggc
//
LOCUS       DASSNN010000554         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1843040, whole genome shotgun sequence.
ACCESSION   DASSNN010000554 DASSNN010000000
VERSION     DASSNN010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843040"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFL84_10310"
     CDS             complement(<1..402)
                     /locus_tag="VFL84_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7609059.1"
                     /translation="MNPATGRVIGRYERHSQSELERRLQRAADEQRAWRETSFADRAA
                     RFRSIAARLRERAPVIAELIASEMGKPRAQGVAEAEKCASVCDYYAALAETFLAPEIV
                     KTEAAESRVVYRPLGPVLCLMPWNFPFWQLFR"
     gene            534..1922
                     /locus_tag="VFL84_10315"
     CDS             534..1922
                     /locus_tag="VFL84_10315"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HET7609060.1"
                     /translation="MHRHRLVLATLIASTALPALAQTPAAPTLDFEFFKARVQPILTT
                     KRDGNARCISCHGFGTTMRLQPLPEGAATWSDADAHKNFEILSARVLPGDPEGSRLLK
                     HPLAEAAGGDPHHDGGKHWTSKNDPEWQTLAAWAGGATLAAETAPKLNVRIVQTNSAG
                     DNVHIIDPATNKVVGTVHGIEVGHGAAAAPDGRWLYVSNEADSTLDVVDARTLRVTSK
                     VPLTGHPNNISISRDGRRVYVAIRAEPGAVDVIDTPTLTRAKSIPIEGAVHNTYVTPD
                     GRYVVAGSIAGKTATAIDQRTEEPAWVMHFDLGVRPMAFEQNADGSTKRMFVQLTEIN
                     GFAVVDFATHKEVQRIELPKLGPGKEPVYEGGNASHGMAVSADNKRLVVDSRLNSAVY
                     VYSLPDLKLQGSVDVGVAPDWVTLTPDGRTAYVANAGSNSVSVVDVVNMREVTRIPVG
                     QVPKRNITARLP"
     gene            complement(1933..>2278)
                     /locus_tag="VFL84_10320"
     CDS             complement(1933..>2278)
                     /locus_tag="VFL84_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HET7609061.1"
                     /translation="KGATTFTMTVREVGRDYRVAIANMGSINPGVTVSGMPKYPTIAD
                     DYARTFAAQKALPVDVFLASHASQFGMHRKYKPGDAYDPNRFVDPKGYLAAVERLEGI
                     YREQLARERAAR"
BASE COUNT          397 a          794 c          751 g          336 t
ORIGIN      
        1 cctgaagagc tgccagaacg ggaagttcca cggcatcagg cagagcacgg ggccgagtgg
       61 gcgatagacg acgcggctct cggccgcttc ggtcttcacg atctccggcg ccagaaacgt
      121 ctcggcgagc gcggcgtagt aatcgcagac ggaggcacac ttctctgcct cggccacacc
      181 ttgcgcgcgc ggcttgccca tctcgctcgc gatgagctcg gcgatgacgg gcgcgcgctc
      241 gcgcaagcgc gccgcgatgg agcgaaacct cgccgcgcga tcggcgaaag aggtttctcg
      301 ccaagcgcgc tgctcgtctg ccgcgcgctg gagccgccgc tcgagctcgc tctgtgagtg
      361 acgctcgtag cgcccgatca cgcggcccgt agcgggattc acgctttcaa ccggcatggc
      421 gcagagcgag caagaatggt gccagccgca tccagccgcc gtcccgtcgc cgctcgattc
      481 gccttcgctc gcggcgcata ctcaatggcg acgatccttc ggggggaaac gtcatgcacc
      541 gccatcgact cgtgctcgcc acgttgatcg cgtcgaccgc cctgcccgcg ctcgctcaga
      601 cgcccgccgc gccgacgctc gacttcgagt tcttcaaagc tcgcgtccag ccgatcctga
      661 cgacgaagcg cgacggcaac gcgcgctgca tctcgtgcca cggcttcggc acgacgatgc
      721 gcttgcagcc gctgccggaa ggcgcggcga cctggagcga cgccgacgcg cacaagaatt
      781 tcgagatttt gagtgcgcgc gttttaccgg gtgacccgga gggcagccgc ttgctgaagc
      841 atccgctcgc ggaagcggca ggcggcgacc cgcatcacga cggcggcaag cactggacct
      901 cgaagaacga cccggaatgg cagacgctcg ccgcttgggc gggcggcgcg acgctcgcgg
      961 cggagaccgc gccgaaattg aacgtacgca tcgtccagac caacagcgcc ggcgacaacg
     1021 tgcacatcat cgatcccgcc acgaacaaag tcgtcggcac ggtccacggc atcgaggtcg
     1081 ggcacggcgc ggcggccgca ccggacggcc gctggctcta cgtcagcaac gaagcggaca
     1141 gcacgctcga cgtcgtcgac gcgcgcacgc tgcgcgtcac gtcgaaagtg ccgctgacgg
     1201 gccacccgaa caacatctcg atcagccgcg acggccgccg cgtctacgtc gcgattcgcg
     1261 cggagcccgg cgccgtcgac gtcatcgaca cgccaacgct gacgcgcgct aagagcattc
     1321 cgatcgaggg tgccgtccac aacacctacg tgacgcccga cggccgctac gtcgtcgccg
     1381 gctcgatcgc gggcaagacg gcgacggcca tcgaccagcg cacggaggag cccgcgtggg
     1441 tcatgcactt cgacctcggc gttcggccga tggcgttcga gcaaaacgcc gacggctcga
     1501 cgaaacgcat gttcgtgcag ctgaccgaga tcaacggctt cgccgtcgtc gacttcgcga
     1561 cgcacaaaga agtgcaacgc atcgagttgc ccaagctcgg gcccggcaag gaaccggtct
     1621 acgaaggcgg caacgcgtcg cacggcatgg ccgtgtcggc cgacaacaag cgcctcgtcg
     1681 tcgacagccg cttgaacagc gcggtgtacg tctattcgtt gccggacttg aagctgcaag
     1741 gctcggtcga cgtcggcgtc gcgcccgact gggtcacgct gacgccggac ggccgcacgg
     1801 cctacgtcgc gaacgcgggc tcgaactcgg tctcggtcgt cgacgtcgtc aacatgcgcg
     1861 aggttacgcg cattccggtc gggcaagtgc cgaagcgcaa catcacggcg cggctaccgt
     1921 agcgcgcgcc ggtcaacgcg ccgcgcgctc gcgcgcgagc tgctcgcggt agatgccttc
     1981 gaggcgctcg acggcggcga gataaccctt cggatcgacg aaccggttcg ggtcgtaggc
     2041 gtcgccgggc ttgtacttcc ggtgcatgcc gaactgcgac gcgtgcgagg cgagaaacac
     2101 gtcgacgggc aacgccttct gcgccgcgaa cgtgcgcgcg tagtcgtcgg cgatcgtcgg
     2161 atacttcggc atgccgctca ccgtgacgcc gggattgatg ctgcccatgt tcgcgatggc
     2221 gacgcgatag tcgcggccta cctcgcgcac cgtcatcgtg aaggtcgtcg cccctttc
//
LOCUS       DASSNN010000555         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1226499, whole genome shotgun sequence.
ACCESSION   DASSNN010000555 DASSNN010000000
VERSION     DASSNN010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226499"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VFL84_10325"
     CDS             complement(<1..784)
                     /locus_tag="VFL84_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609062.1"
                     /translation="MTNTRRSFDPIKLACWAGIAVYGVEKAVRLQQGAALGELFDLGD
                     PSLWALLIGACVVGLILRSPTMRRRAMLIEANETRAATMSFVTVFTVMAVYYILRPVR
                     DSLASEWTDLEISHLWTIQFFLSLALVMVYGFACSRIRFRLLVPAVYAFYAVTFALFY
                     VGSSFVADRVLLDKAFYVWVSLFSLFHLSVFWSFMADTFTTEQSKRLFPFIAAGASAG
                     AFAGPSFVAVIASEVGNDNLMLVGAATLLLAIPMILWIQRLKV"
     gene            complement(1126..2070)
                     /gene="glk"
                     /locus_tag="VFL84_10330"
     CDS             complement(1126..2070)
                     /gene="glk"
                     /locus_tag="VFL84_10330"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HET7609063.1"
                     /translation="MDLIADIGATTTRCALVDDKGQELAPEIFENADFTGVVGVLRVY
                     LDHRRASDRPVRAALAVAAPITGDDVHMVNIGWPFSQTALRKELGLKRLQVVNDFAAI
                     AWALPKLTPADVVKIGGGESVSHATLAALGPGSGLGVSALVPCADAWAVMSGEGGHVS
                     MPAITREEEVVIAALRDRFDGHCSAERVLSGPGLVNLYVTLAELAGRGRPTVLPEDVT
                     NLAKQGEPLARKTLAMFFAMLGTIAANLAVTTGARGGVYIAGGIVPRLVEPLRKSEFR
                     ARFEAKGRYRDYLAAIPTYLITAQLPAFRGLKYLLGYS"
     gene            complement(2082..2297)
                     /locus_tag="VFL84_10335"
     CDS             complement(2082..2297)
                     /locus_tag="VFL84_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HET7609064.1"
                     /translation="MTDKIETEIFRNVERLKQLRVEHRDLDQVIARLVTDPNVDQLML
                     KRLKKRKLMLKDMITQLESARIPDLNA"
BASE COUNT          448 a          752 c          785 g          380 t
ORIGIN      
        1 cgacttttag gcgctgaatc cataggatca tcgggatcgc gagtaacaga gtcgcggcgc
       61 ccacgagcat caagttgtcg ttgccgacct cgcttgcgat gacggccacg aaactcggtc
      121 ccgcgaaggc accggcgctc gcgccggccg cgatgaatgg aaacagccgc ttcgactgct
      181 ccgtcgtgaa ggtgtctgcc atgaagctcc agaacaccga cagatggaac aggctgaaca
      241 ggctgaccca gacatagaag gctttgtcga gcagcacgcg gtcggcaacg aagctcgagc
      301 cgacgtagaa gagcgcgaac gtcaccgcgt agaacgcgta aaccgcgggc acgagcaggc
      361 ggaagcgaat cctcgagcac gcgaagccat acaccatcac gagcgcgagg ctcaagaaga
      421 actgaatcgt ccacaggtgc gaaatttcga ggtccgtcca ctcgctcgcc agcgagtccc
      481 gcacggggcg caggatgtaa tagacggcca tgaccgtgaa gacggtcacg aacgacatcg
      541 tggcggcacg ggtttcgttc gcctcgatca gcatcgctct gcgccgcatc gtcggcgacc
      601 gcaagatcaa gccgacgacg cacgcgccga tgagcagcgc ccagagtgaa gggtcaccga
      661 ggtcgaacag ctcgccgagc gccgcgccct gttgcaggcg caccgccttc tctacgccat
      721 agaccgcaat gcccgcccag caggcgagct tgatcggatc gaacgaccgc ctggtgttag
      781 tcatcgacgg agaatacacc gcgccttgcg gcggctgata cggcggggag ggtgccgcaa
      841 gtctcggttt ttcccaagcg ctggtgctag gttacgaacg cggtgctcga ggagggctcg
      901 ttcaatgcca ggaaaagcag cggcgctcat tgcgatcgtg tgtgtccttg gcggctgcag
      961 cggttcggaa gagccggccg cgcgcgccac ggagccggaa acggcagaga agcgggaaac
     1021 ggtgttcgat cctttgacga gcacgatcga ccgcgcggaa ggcgtgcagc aaaccgtcga
     1081 cgagcaagcg gccgagcagc gccggcggat cgaggaagcc tcgcgttaag agtagccgag
     1141 caggtatttg aggccgcgga acgcgggcag ctgcgccgtg atcaagtagg tcgggatcgc
     1201 cgcgaggtaa tcgcggtacc ggcccttggc ctcgaagcgc gcgcgaaatt cggatttgcg
     1261 cagcggctcg acgaggcgcg gcacgatgcc gccggcgata tagacgccgc cgcgcgcgcc
     1321 cgtcgtcacg gcgagattgg ccgcgatcgt gccgagcatg gcgaaaaaca tcgcgagcgt
     1381 cttgcgggcc agcggctctc cctgtttcgc caggttcgtg acgtcctcgg gcaagacggt
     1441 cggacggccg cgccccgcga gctcggcgag cgtgacgtat aggttcacga ggcccggccc
     1501 cgaaaggacg cgctccgccg agcagtgccc gtcgaacctg tcgcgcaggg ccgcgatgac
     1561 aacctcctcc tcgcgcgtga tggccggcat cgagacgtga ccgccttcac cgctcatcac
     1621 tgcccaagcg tcggcgcaag gcacgagcgc cgagacgccg aggccggaac ccgggccgag
     1681 cgcggcgagc gtcgcgtgcg acacgctctc gccgccgccg atcttgacta cgtcggctgg
     1741 cgtcagcttc ggcagcgccc acgcgatcgc cgcgaaatcg ttgacgactt gcagccgctt
     1801 caggccgagc tccttgcgca acgcggtctg cgagaacggc cagccgatgt tgaccatgtg
     1861 cacgtcgtcg cccgtgatcg gcgcggcgac ggccagcgcc gcgcggaccg gccgatcgct
     1921 cgcgcggcga tggtcgagat acacgcgcag cacgccgacg acgccggtga agtcggcgtt
     1981 ctcgaagatt tcgggcgcga gctcttgccc tttgtcgtcg accagtgcac agcgcgtcgt
     2041 ggtcgcgccg atgtcggcga tcaagtccat ttgcgcactc gtcaggcgtt gaggtccgga
     2101 atcctggcgc tctcgagctg agtgatcatg tccttgagca tcagcttgcg tttcttgagc
     2161 ctcttgagca tgagctgatc cacgttcgga tcagtgacga gccgggcgat gacctggtcc
     2221 aagtcccggt gctctacgcg tagttgcttg agccgctcca cgttcctaaa gatctcggtt
     2281 tcgattttgt cggtcacttg ccctctggat gggtgccaac cagtcgcgcc gctggtcgcc
     2341 aaaggccagg acgtgaggct ttaac
//
LOCUS       DASSNN010000556         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_600055, whole genome shotgun sequence.
ACCESSION   DASSNN010000556 DASSNN010000000
VERSION     DASSNN010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600055"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VFL84_10340"
     CDS             <1..443
                     /locus_tag="VFL84_10340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7609065.1"
                     /translation="ASEVALVETVRASMRTSAYTLAGCLDFAELCAVIEAADVLVSNN
                     TAPVHIAGAVGTPVVDLYALTNPQHVPWRVPHRVLYRDVPCRYCYKSVCPAGHHDCLA
                     QVSPAEVADAVRGLLDSTFEPVRATLDALPLTNLERPVAPRASV"
     gene            457..2166
                     /locus_tag="VFL84_10345"
     CDS             457..2166
                     /locus_tag="VFL84_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HET7609066.1"
                     /translation="MYTLGINAAYHDSAACLVRDGDVVAAAEEERFTRIKHGKRPVPF
                     TAWELPYHAIDYCLAAAGIELADLDDIAYSYDPYLLLARRGASVTLPLEPSAGAKGGL
                     EPWEPLFVSYIVNAERQLVDGVPHRLRERFVTAKERRHWKWHFVEHHLAHEASAFLPS
                     PFADAAVLTLDGRGEKTATSYGHFADGAYRRLKQVQLPSSLGLLYEQATDYLGFLRSS
                     DEYKVMALASYGKPTYVDWFRDVVELTGEGGYVVHPARFEDRFGPPRSRGAAVTQHHF
                     DIAHSLQAVLEEAVSELARWLRGETGSRNLCMAGGVALNCVLNGRLRAQRIFDRIFVQ
                     PAAGDAGTALGAALWIDWCHGARERRFRMDHAYLGPEFSDDEIEAELKRSKQKYRRAA
                     DVADEAARLLAAERILGWFQGRMEFGPRALGARSILASPLKADMQQRLNEIKDREDFR
                     PVAPAVLEEAAADWFVDAERAPFMLFVFDVAPGKAHLIPAVRHVDGTARVQTVGAHEQ
                     PRYYELLKAFERRTGVPVLVNTSFNSRGDPVVCTPRDALESFWSTPLDALAIGSFIIE
                     KPS"
BASE COUNT          325 a          776 c          744 g          362 t
ORIGIN      
        1 acgcgagcga ggtcgcgctc gtcgagaccg tgcgcgcgtc gatgcgcacg agcgcctaca
       61 cgctcgcggg ctgcctcgac ttcgcggagc tctgcgccgt catcgaagcg gccgacgtgc
      121 tcgtctcgaa caataccgcg cccgtgcaca ttgccggcgc cgtcggcacg ccggtcgtcg
      181 acctctacgc gctgacgaat ccgcagcacg tgccttggcg cgtgccgcat cgcgtgctct
      241 atcgcgacgt gccgtgccgc tattgctaca agagcgtctg tcccgccggt caccacgact
      301 gcttggcgca agtctcgccg gccgaggtcg ccgatgccgt ccgcgggctt ctcgactcga
      361 cgttcgagcc cgtgcgcgcg accctcgacg cgcttccact caccaatctg gaacgccccg
      421 tcgcgccgcg cgcgagcgtg tagcaggcgg ggctcgatgt acacgctcgg catcaacgct
      481 gcctaccacg attcggccgc gtgcctcgtg cgcgacggtg acgtcgtcgc ggcggccgag
      541 gaagagcgct tcacgcgcat caagcacggc aagcggcccg ttcctttcac ggcgtgggag
      601 ctgccgtatc acgccatcga ctattgcctc gcggccgcgg gcatcgagct cgccgatctc
      661 gacgatatcg cctattcgta cgatccgtac ctgctgctcg cccggcgcgg cgcgagcgtc
      721 acactgcccc tcgagccgag cgccggcgcg aaagggggcc ttgagccgtg ggagccgctg
      781 ttcgtctcgt acatcgtcaa cgcggagcgg cagctcgtcg acggcgtgcc gcatcggctc
      841 cgcgagcggt tcgtaacggc caaggagcgc cggcattgga agtggcattt cgtcgagcac
      901 catctcgcgc atgaggccag cgcgttcttg ccgtcgccgt tcgcggatgc cgcggtgttg
      961 acgctcgacg gccgaggcga gaagaccgcg acgagctacg gccatttcgc cgacggcgcc
     1021 taccggcgct tgaagcaagt gcagctgccg agctcgctcg gcttgctgta cgagcaggcc
     1081 actgactatc tcggcttcct gcggtcgtcc gacgaataca aggtcatggc gctcgcgtcg
     1141 tacggcaagc cgacctacgt cgactggttt cgcgacgtcg tggagctgac gggcgagggc
     1201 ggctacgtcg tgcatccggc gcggttcgag gaccgcttcg gaccgccgcg ctcgcgcggg
     1261 gcggcggtca cgcagcacca cttcgacatc gcgcattcgt tgcaggcggt gctcgaggag
     1321 gccgtcagcg agctcgcgcg ctggctgcgc ggcgagacgg gctcgcggaa cttgtgcatg
     1381 gccggcggag tcgcgctgaa ctgcgtgctg aacgggcgcc ttcgagcgca gcggattttc
     1441 gaccgcatct tcgtgcagcc cgccgccggc gatgccggaa cggcgctcgg cgccgcgctc
     1501 tggatcgact ggtgccacgg cgcgcgggag cggcgctttc gcatggacca cgcgtatctc
     1561 ggccccgagt tcagcgacga cgagatcgag gccgagctga agcgctcgaa acagaaatac
     1621 cggcgcgcgg ccgacgtcgc cgacgaggcg gctcggctgc tcgccgcgga gcgaattctc
     1681 ggctggtttc agggccgcat ggagttcggt ccgcgcgcgc tcggcgcgag gtcgatcctc
     1741 gcctcgccgc tcaaggccga catgcagcag cggctcaacg agatcaagga ccgcgaggat
     1801 tttcgccctg tcgcgccggc cgtgctcgag gaggccgccg cggactggtt cgtcgacgcc
     1861 gaacgcgcgc cgttcatgct gttcgtgttc gacgtcgcgc ccggcaaggc gcatttgatc
     1921 ccggccgtcc gccacgtcga cggcacggcg cgcgtgcaaa cggtcggcgc gcacgagcag
     1981 ccgcgctact acgaacttct gaaagcgttc gagcgccgca ccggcgttcc agtgctcgtc
     2041 aacacgtcgt tcaacagccg cggcgacccc gtcgtctgca cgccgcgcga cgcgctcgag
     2101 agcttctgga gcacgccgct cgacgcgctc gcaatcggtt ctttcatcat cgagaaaccg
     2161 tcatgaagcc acgtgtatcg gtcgtcgtcc ctacgcacgg caggccc
//
LOCUS       DASSNN010000557         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1843246, whole genome shotgun sequence.
ACCESSION   DASSNN010000557 DASSNN010000000
VERSION     DASSNN010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843246"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..992
                     /locus_tag="VFL84_10350"
     CDS             42..992
                     /locus_tag="VFL84_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7609067.1"
                     /translation="MDGITSRRQFLTKALGATAAVVGAGALRGAAAQSAPALGETELR
                     NDLSLVTGLPTNVVVLRTAGSAALVDSGPASHAAELAKLVRGRLGFLSVELLFNTHWH
                     VAHTGGNEALRSGDTTIIAHELTRLWMSTEYYVDWEDTTYEPREHAALPTRTFYATDP
                     QPIVAEIGDEEIEYGHLPEAHTDGDIYVWFKNRNVLAAGGVVTHGSYPVIDYATGGWI
                     GGLVDATRKLLAMTSSDTLIVPASGPAQPRAHLTRQLEMLTVVRERIENLMRKGRSIE
                     EMLAAEVTKEFDSAWGDNRERFVANVYNGLWWAGRLSDSL"
     gene            979..>2708
                     /locus_tag="VFL84_10355"
     CDS             979..>2708
                     /locus_tag="VFL84_10355"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET7609068.1"
                     /translation="MTRFERDCASRAAAALAAALLAGCSEPPPDQAVARHIELFGRYC
                     TECHNDAEAAGDMSLEHVTPELIAAKPEVFEQVIRKLRGGLMPPPGEPRPDREDRLAL
                     VTALERHLDATAAARGPKPGRVALHRMNRTEYAAAVEQLLALRIDARSMLPADMASEG
                     FDNVAEVLRVTPTHIEQYVAAARDISIMAVGEKAPAPTRADYRSERGLRTAHVDGLPL
                     GTRDGLLVEHVFPADGTYEINLNISSIPGSELRGYPYGWLEYEHTVAVTIDGAKVFLG
                     NIGGEADSKALDQGQIKEVEAIRNRFRGIRVDVKAGRRSVGAAFVARSFAEGDYLLQS
                     LVPGEGVPDIPRLYGMEIIGPYDPTGIAEPTESRARIFSCYPQSESEEQPCATEILTS
                     LARGAFRRPVTSEDVAPALEFYAQGRAEGGFETGIQKGLMAILASTRFLYRADPGAPP
                     ANVEPGSLYAISDLELAWRLSFFLWSQGPDDALLKLAEDGTLHEPDVLDAQVRRMLAD
                     ERSRSLVTNFAFQWLGVRRLEAIDPDPRLYPTFDEDLRRAFTKEMELYLDSILRERAS
                     VVDLLTAKHT"
BASE COUNT          447 a          951 c          935 g          375 t
ORIGIN      
        1 cggcgtgcag ccgagtagcg cgactcatta atataggaaa catggacggg atcacgagcc
       61 gccggcaatt cctgacgaag gcgctcggcg cgacggcggc cgtcgtcggc gcgggagctc
      121 tgcgcggcgc ggccgcgcaa agcgcgcccg cgctcggcga aacggagctt cgcaacgacc
      181 tctcgctcgt cacgggcctg cccacgaacg tcgtcgtgct gcgcacggca gggtccgcgg
      241 cgctcgtcga cagcggcccg gcctcgcacg cggccgagct cgcgaagctc gtgcgcggca
      301 ggctcggctt cctgtcggtc gagctgctgt tcaacacgca ctggcatgtc gcgcacacgg
      361 gcggcaacga ggcgctgcgc tccggcgaca cgacgatcat cgcgcacgag ctcacgcggc
      421 tctggatgag cacggagtac tacgtggact gggaagacac gacgtacgag ccgcgcgagc
      481 acgccgcgct gccgacgcgc acgttctacg cgaccgaccc gcagccgatc gtcgccgaga
      541 tcggcgacga ggagatcgag tacggccacc tgcccgaggc gcatacggac ggcgacatct
      601 acgtctggtt caagaaccgc aacgtgctcg ccgcgggcgg cgtcgtgacg cacggctcgt
      661 atcccgtcat cgactacgcg accggcggct ggatcggcgg gctcgtcgac gcgacgcgca
      721 agctgctcgc aatgacgagc tccgacacgc tgatcgtgcc ggcgagcgga cctgcgcagc
      781 ctcgcgcgca tcttacgagg cagctcgaga tgctgaccgt cgtgcgcgag cgcatcgaaa
      841 acctgatgcg caaaggccgc agcatcgagg agatgctcgc ggcggaagtc accaaggaat
      901 tcgactcggc gtggggcgac aaccgcgaac gcttcgtcgc gaacgtctac aacgggctct
      961 ggtgggcagg gaggctcagt gactcgcttt gagcgtgact gcgcgtcccg cgccgccgcg
     1021 gcgttggccg ccgcgctgct cgcgggctgc tcggagccgc cgcccgacca ggccgtcgcg
     1081 cggcacatcg agctcttcgg ccgctattgc accgaatgcc acaacgacgc ggaagccgcc
     1141 ggcgacatgt cgctcgagca cgtgacgccg gagctgatcg ccgcgaagcc cgaagtgttc
     1201 gagcaggtca tccgcaagct gcgcggcggg ctcatgccgc cgcccggcga gccgcgcccc
     1261 gacagggagg accggctcgc gctcgtgacg gcgctcgagc ggcacctcga cgcgacggcc
     1321 gcggcacggg ggccgaagcc cgggcgcgtc gcgctgcacc gcatgaaccg caccgagtac
     1381 gccgcggccg tcgagcagct gctcgcgctt cgcatcgacg cgcgctcgat gctgcccgcc
     1441 gacatggcga gcgaaggctt cgacaacgtg gccgaggtgc tgcgcgtcac gccgacccac
     1501 atcgagcagt acgtcgccgc cgcgcgcgac atcagcatca tggccgtcgg cgagaaggcg
     1561 cccgcgccga cgcgcgcgga ctatcgctcg gagcgcgggc tgcgcacggc gcacgtcgac
     1621 ggcttgccgc tcggcacgcg cgacggtttg ctcgtcgagc acgtcttccc ggccgacggc
     1681 acgtacgaga tcaacctcaa catctcgtcg ataccgggct cggagctgcg cggctatccg
     1741 tacggctggc tcgagtacga gcacaccgtc gccgtgacga tcgacggcgc gaaggtgttc
     1801 ttaggcaaca tcggcggcga ggccgactcg aaggcgctcg accaggggca gatcaaggag
     1861 gtcgaggcga tcaggaaccg tttccgcggc attcgcgtcg acgtcaaggc gggccggcgc
     1921 agcgtcggcg ccgcgttcgt cgcgcgcagc ttcgccgaag gcgactatct gttgcagtcg
     1981 ctcgtgcccg gcgaaggcgt gcccgacatc ccgcggctct acggcatgga aatcatcggc
     2041 ccgtacgacc cgaccggcat cgcggagccg accgaaagcc gcgcgcgcat cttcagctgc
     2101 tacccgcaga gcgagagcga ggagcagccg tgcgcgaccg agatcctgac gagcttggcg
     2161 cgcggcgcgt tccgccggcc cgtgacgagc gaggacgtcg cgccggcgct cgagttctac
     2221 gcgcaaggcc gagccgaagg cggcttcgag accggcatcc agaaggggct catggcgatt
     2281 ctcgcgagca cgaggttcct ctatcgcgcc gacccgggcg cgccgccggc gaacgtcgag
     2341 ccgggctcgc tttacgcgat cagcgacctc gagctcgcgt ggcggttgtc gtttttcctg
     2401 tggagccaag gccccgacga cgcgctgctg aagctcgccg aagacggcac gctgcacgag
     2461 cccgacgtcc tcgacgctca agtgcgccgc atgctcgccg acgagcgctc gcgctcgctc
     2521 gtcactaatt tcgcgttcca gtggctcggc gtgcgccggc tcgaggcgat cgatcccgac
     2581 cctcgcctgt atccgacgtt cgacgaagac ctgcgccgcg cattcacgaa ggagatggag
     2641 ctctatctcg acagcatcct gcgtgagcgt gcgagcgtcg tcgatctgct cacggcgaag
     2701 cacacgtt
//
LOCUS       DASSNN010000558         4074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1609487, whole genome shotgun sequence.
ACCESSION   DASSNN010000558 DASSNN010000000
VERSION     DASSNN010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609487"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VFL84_10360"
     CDS             <1..456
                     /locus_tag="VFL84_10360"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HET7609069.1"
                     /translation="NADGAAIAAAVARGENQTFVIDGEALTIEPNDLLVESSAAEGFA
                     CAEEAGYLVGLDTTLDDALRREGLARELVRAVQDARKQAGLEVADRIVLTVEGDAAVT
                     AAVAEHRDYLMTETLASAWRKPGKDEFVATQSQGEARWVIRLARDRGSR"
     gene            453..995
                     /gene="lspA"
                     /locus_tag="VFL84_10365"
     CDS             453..995
                     /gene="lspA"
                     /locus_tag="VFL84_10365"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004930972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET7609070.1"
                     /translation="MTERRWFSLSAGASNWLWLGVAVMALDQWTKWLIVERFEEYEPY
                     VLLPVLEIMRLHNTGAAFSFLSNASGWQRWVFVGLGVAVSAGILVWLRRLPPKGQGLL
                     AAGLSLILGGALGNVIDRVLRGHVIDFIRVHYHDHAFPAFNVADSAITIGAALIVLDN
                     LLELDRLRAKKKSSPTGIGG"
     gene            complement(972..1439)
                     /locus_tag="VFL84_10370"
     CDS             complement(972..1439)
                     /locus_tag="VFL84_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HET7609071.1"
                     /translation="MNTTAHTLGRRQSGQLRSRMRGVTLLELMAVVMVIGVLGMIAIP
                     SYRQYVMRAQRADAKTALLQLQTNQERYYLANRTYGTIAQLTAANLSNSGISERGAYQ
                     ITLTGVSATGYTATAAPRAGGAFDMSGDAQCTTFSIDAQGVRTATGSAADTCW"
     gene            complement(1460..>4074)
                     /locus_tag="VFL84_10375"
     CDS             complement(1460..>4074)
                     /locus_tag="VFL84_10375"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HET7609072.1"
                     /translation="CQKNFIVYLTDGEPTYDNAADTKIRNMTDANGATFSTLVGNTCD
                     AEAWAGIDFASPPGGTQSMCLDDLAEFLRDGDFSPLAGQQNILTYTVGFTVDLPLLAQ
                     TASRGGGSYYTANDAASLSTALQQIVTSILSSNTTFTAPTVAVNAFNRTQNLSDLFIS
                     VFRPSARMHWPGNLKRFRLDATTGEIVDANGDPAIDPATGFFADTARDLWSTSSGGDG
                     EDVEKGGAANLVPNSSSRVVYTYMGTVNKDLTHSDNRVQSSNGLLTDAVLLTGGAGDP
                     TRDQVIDFINGKDLPDTDQDNDVAEARTQLGDPLHSQPVSMVYGPGLREGLIFSATND
                     GMLHAFNLESGREEWAFIPPDFLDDQVQLYMDDPAAAKHYGIDGDLALQVVADNDATI
                     ESGEKVYLFFGMRRGGDFYYGLDVSDRSAPKLLWRIDGTTLPGIGQSWSTPVPTHVNV
                     SGATQNADKLALVIGGGYDPHHDNDAPSDDTVGNSIYIVDAVSGALLWHGGKTGTHKS
                     FNVSGRSMDYAIPSDVRVIDIDGNGYADRMYVGDMGGQVWRFDITNGNNATNLVAGGV
                     IAQLGAAPNWSSATLANTRRFYYAPDVAFISARGYDFIHIGIGSGTRGHPLSTAARDR
                     FYALRDYGWARKTQSDFDSLTPITDSALTAITAANQSVPQGTSPGWRLDLKNGSSWLG
                     EKVLAEARTFNNEVIFTTFRPGTAGTSCVPQLGTNRIYRMSVFNGSPVLNLDGSADPT
                     TLTMSDLYLESEGAPLPAPQMIFVDADRDADGIPDAEDDTDGDGIPDSEDPDYDGNGV
                     VDTNEDMDGDGIPDTQDDDVDGDNIPNSSDPDYRPTLSQSDGIMMAVGVMVFPEGYRN
                     DPVRTYWRQANIGN"
BASE COUNT          619 a         1277 c         1432 g          746 t
ORIGIN      
        1 aatgccgacg gcgcggcgat cgcggccgcc gtcgcgcgcg gcgagaacca gacgttcgtc
       61 atcgacggcg aggcgctgac gatcgagccc aatgacctgc tcgtcgagag ctcggcggcg
      121 gaagggttcg cgtgcgcgga agaggccggt tacctcgttg ggctcgacac gacgctcgac
      181 gacgcgctgc gccgcgaggg actcgcgcgc gagctcgtgc gcgccgtgca ggacgcgcgc
      241 aagcaggccg gtctcgaggt ggcggatcgc atcgtgctga cggtcgaagg cgacgcggcc
      301 gtcacggccg cggtcgccga gcaccgcgac tatttgatga ccgagacgct cgcgagcgcc
      361 tggcggaagc ccggcaagga cgagttcgtc gcaacgcaga gccaaggcga agcgcgctgg
      421 gtcattcggc tcgcgcgcga tcgggggtcg cgatgacgga acgccgctgg ttcagcttga
      481 gcgccggcgc ctcgaactgg ttgtggctcg gcgtcgccgt catggcgctc gatcagtgga
      541 cgaaatggct catcgtcgag cggttcgagg agtacgagcc atacgtgctg ctgcccgtgc
      601 tcgagatcat gcggctgcac aacacgggcg ccgcgttcag cttcttgagc aacgcgagcg
      661 gctggcagcg ctgggtattc gtgggcttgg gcgtcgccgt cagcgccggg attctggtct
      721 ggctgcggcg cttgccgccg aagggccagg ggttgctcgc cgcgggctta tcgctgatcc
      781 tcggcggcgc gctcggcaac gtcatcgatc gcgtgctgcg cgggcacgtc atcgatttca
      841 tccgcgtgca ctaccacgat cacgccttcc cggcgttcaa cgtcgcggac tcggcgatca
      901 cgatcggagc cgcgctgatc gtgctcgaca acctgctcga gctcgaccgg ctgcgggcca
      961 agaaaaaaag ctcaccaaca ggtatcggcg gctgatcccg tagcggtgcg cacgccctgc
     1021 gcatcgatgg aaaaggtcgt gcactgcgcg tcgccgctca tgtcgaacgc gccgccggct
     1081 ctcggcgccg ccgtcgcggt gtagccggtc gcgctgacgc cggtcaacgt gatctggtac
     1141 gcgccgcgct cgctgatgcc gctgttcgac aggttcgccg cggtgagctg cgcgatggtc
     1201 ccatacgtgc ggttcgcgag gtagtagcgt tcctgattcg tttggagctg cagcagggcc
     1261 gtcttggcgt cggcacgctg cgcgcgcatg acgtactggc ggtagctcgg aatcgcgatc
     1321 atgccgagca cgccgatgac catgacgacg gccatgagct cgagcagcgt cacgccgcgc
     1381 atgcgcgaac ggagctgccc gctttgccgc cggccgaggg tgtgtgcagt ggtattcatc
     1441 gattgcatcc cgcgaagcct cagtttccga tgtttgcctg gcgccagtac gtgcggacgg
     1501 ggtcgttgcg gtagccctcc gggaagacca tgacgccgac ggccatcatg atgccgtcgc
     1561 tctgcgacaa cgtcggccgg tagtccggat ccgagctgtt cggaatgttg tcgccgtcga
     1621 cgtcgtcgtc ctgggtgtcc gggatgccgt cgccgtccat gtcctcgttc gtgtccacga
     1681 cgccgttgcc gtcgtagtcg ggatcctcgc tgtcggggat gccgtcgccg tcggtgtcgt
     1741 cctctgcgtc gggaatgccg tccgcatccc gatcggcgtc gacgaagatc atctgcggcg
     1801 ccggcagcgg cgcgccctcg ctctcgaggt acaagtcgct catcgtcaac gtcgtcgggt
     1861 ccgcagatcc gtcgaggttc aaaaccggcg acccgttgaa gacgctcatg cgatagatgc
     1921 ggttcgtgcc gagctgcggc acgcaagagg tgcctgccgt gccggggcgg aacgtcgtga
     1981 agatgacctc gttgttgaag gttcgcgcct ccgcgagcac cttctcacct agccaactcg
     2041 agccgttctt gaggtcgaga cgccaacccg ggctcgtgcc ctgcgggacg ctctggttcg
     2101 ccgcggtaat ggcggtcagc gctgaatccg tgatcggcgt cagggagtcg aagtccgatt
     2161 gcgtcttcct cgcccaccca tagtcgcgca gcgcatagaa ccggtcgcga gcggcggtgc
     2221 tgagcgggtg gccgcgcgtg ccggagccga tgccaatgtg gatgaagtcg tagccgcggg
     2281 cgctgatgaa tgcgacgtcg ggcgcgtagt aaaagcggcg ggtgttggca agcgtggccg
     2341 aggaccagtt cggcgccgcg ccgagttgcg cgatgacgcc gcccgccacg aggttcgtgg
     2401 cattgttgcc gttcgtgata tcgaaccgcc acacctggcc gcccatgtcg ccgacgtaca
     2461 tgcggtcggc gtagccgttg ccgtcgatgt cgatgacgcg gacgtcggac gggatcgcgt
     2521 agtccatgga gcgcccggag acattgaagc tcttgtgcgt gccggtcttg ccgccgtgcc
     2581 agagcagggc gccgctcacc gcgtcgacga tgtagatcga gttgccgacc gtgtcgtcgg
     2641 acggcgcatc gttgtcgtga tgcgggtcgt agccgccgcc gatgacgagc gcgagcttgt
     2701 cggcgttctg cgtagcgccg ctcacgttga cgtgcgtcgg gacgggcgtc gaccagctct
     2761 gcccgatacc gggcagcgtc gtgccgtcga tacgccacag caatttcggc gcggaccggt
     2821 cgctgacgtc gaggccgtag tagaagtccc cgccgcggcg catgccgaag aacaggtaga
     2881 ccttctcgcc gctctcgatg gttgcgtcgt tgtcggcgac gacttgcagg gcgaggtcgc
     2941 cgtcgatgcc gtagtgcttc gctgcggccg ggtcatccat gtatagctgg acttggtcgt
     3001 cgagaaagtc gggagggatg aatgcccatt cctcgcgtcc gctctcgaga ttgaaggcat
     3061 gcagcatgcc gtcgttcgtc gccgaaaaaa tcagaccctc gcgcagcccc ggaccgtaaa
     3121 ccatcgacac cggctgcgag tgcagcggat cgccgagctg agtgcgcgcc tcggcgacgt
     3181 cgttgtcctg gtccgtgtcg ggcaggtcct tgccgttgat gaagtcgatg acttgatcgc
     3241 gggtcggatc gcccgcgccg cccgtcagca ggacggcgtc ggtgagcaat ccgttggacg
     3301 actgcacgcg gttgtcgcta tgcgtcaggt ccttgttgac cgtgcccatg tacgtgtaca
     3361 cgacgcggct cgaggagttc gggacgaggt tcgcggcgcc gcctttttcg acgtcctcgc
     3421 cgtcaccgcc gctcgacgtc gaccacaggt cgcgcgccgt gtccgcgaag aagcccgtcg
     3481 ccggatcgat ggccggatcg ccattggcgt cgacgatctc gcccgtcgtc gcgtcgagac
     3541 ggaatcgctt gaggttgccc ggccagtgca tgcgcgcgga cggacggaat acgctgatga
     3601 agagatcgct gagattctgc gtgcggttga acgcgttcac ggcgacggtc ggcgcggtga
     3661 aggtcgtgtt cgacgacagg atcgaagtca cgatctgctg tagcgcggtc gacaggctgg
     3721 ccgcgtcgtt ggccgtgtaa taggaaccgc cgccgcgcga cgccgtttgg gcgagcaacg
     3781 gcaagtcgac ggtgaagccg accgtgtagg tcaggatgtt ctgctgcccc gcgagcggcg
     3841 agaaatcgcc gtcgcgcagg aactcggcga ggtcgtcgag gcacatggat tgcgtgccgc
     3901 ccggcggcga cgcgaagtcg atgccggccc aggcctccgc gtcgcaggtg ttcccgacca
     3961 gcgtcgagaa cgtggcccca ttggcgtcgg tcatgttgcg gattttcgta tccgccgcgt
     4021 tgtcgtaggt cggttcgccg tcggtcaggt acacgatgaa gttcttctga cagc
//
LOCUS       DASSNN010000559         5339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_959460, whole genome shotgun sequence.
ACCESSION   DASSNN010000559 DASSNN010000000
VERSION     DASSNN010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959460"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFL84_10380"
     CDS             <1..294
                     /locus_tag="VFL84_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7609073.1"
                     /translation="RAHEFSIRTAMGAGRRDIVLLVLREGLIVTVVGILIGIVAAFGL
                     TRLIASRLYDVTPTDPLTIVAVVVLLTVIAMLACVIPARHAANAEPLRALRVD"
     gene            435..1589
                     /locus_tag="VFL84_10385"
     CDS             435..1589
                     /locus_tag="VFL84_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET7609074.1"
                     /translation="MAEDAFNHALRGKVAPGSELDAEPFGSSRSFKRPLVVCLVGLVA
                     LGVALQSLLIWRGGDSTQRASLLAAQAPTFEIEMPGTGRNLLLSVAELRWCLREDLRI
                     EIFEQRLSYSEPQRFGVMVADYNRRCTRFKYRDNDLERARRDIDGARKWIVEEAFAEA
                     YSTAAGGAPDRLAANGYSILTKDVQRLLRAVGYDPGPIDGHYGPRTRAAVESFEAEVG
                     RDPTGGISEALRRELLDRVRSGGFAESRLLAATAAEAATIRASCAAANGVAAYNRCVE
                     SELAALAQRRPPPARAMTAAERDTIDEVCLRAKLRGGAAGYDRCVEEQIAELAALESE
                     PSVAAMTRRQRATVEDRCASTGSFYGPAAFYRCAQERLAEVAAAGRNASL"
     gene            1658..2437
                     /locus_tag="VFL84_10390"
     CDS             1658..2437
                     /locus_tag="VFL84_10390"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET7609075.1"
                     /translation="MKRRMRMFVVALTLSASASIVFAQFFRRGYSPGVDQPLVGEPKE
                     WTFARLAYDGTGMRGRSGWSVDYPYAEIHFSDAVDRLTRIDVHPDGHVISPDSDDLFN
                     YPWLYAVEVGSWTFSDSQAARMREYLLRGGFLMVDDFHGEYEWQVFVEGMRMIFPDRP
                     IEDIPATDVIYSQPYEIEKRVQVPGPDYMTTGLTYERSDGVTPHWRGIRDADGRWMVM
                     ITHNIDYGEGWEQADNPRYPEAFTRQAYEVAVNYLIYTMTH"
     gene            complement(2471..3418)
                     /locus_tag="VFL84_10395"
     CDS             complement(2471..3418)
                     /locus_tag="VFL84_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7609076.1"
                     /translation="MPFIKVTDLAYGRLQSPNLDEAEEFLTAFGLVRTERTRDALYMR
                     GTDPSHHVHVTHLGPSRFMGLGFFAESEDDLARLARAPGASGVEHVDEPGGGKRVSLT
                     DPLGYRVEVVCGMQTLPRLPAAEQSTNTGTNKLRRAGELFRVERGPSRVKRIAHAVLM
                     AKSAADKIRWYREMLGFLPSDEVYEGSKDNIVASFNRCDRGETFVDHHTLLCFESPTT
                     GLNHLSFEVQGFDDLMVGHEHLKQAGKYRHVWGIGRHLLGSQIFDYWEDPWRRVHEHW
                     TDSDVLNARAGAGLHPVEIGFSAQWGTPPPDDFVHHAIP"
     gene            complement(3532..4656)
                     /locus_tag="VFL84_10400"
     CDS             complement(3532..4656)
                     /locus_tag="VFL84_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609077.1"
                     /translation="MKTTLGSIVSLAGMLAASVCMVGSAHAQSDPRAPQFKVDPSWPT
                     IPNDWVLGEVTSISVDRNDNVWVLHVPQSIPEDKRANAAPPVLEFDSAGKLLRSWGGP
                     SNGAAWLGREHGIFVDANDFVWIGGRAGWPRQTTPGVSDDMIMKFTMTGELVMQIGRS
                     GASTGNLDTENVHQATDLFVDTRAKEVYAADGYGNKRVIVFDSETGKFKRMWGAFGNP
                     PPATFAANAPTPQPQTTPEGPPEFGLPHAIKVSNDGVVYLADRINNRIQLFTREGKFL
                     KQVRVTNEGSDVVPVPAGFAFSPDAKQQFLYVVDSGPMRVVIFDRDTMTQIGVVGMKG
                     ANAGEFDIVHHMAVDSKGNLYTAEIVNNRRAQKFVLAGAR"
     gene            complement(4722..>5339)
                     /locus_tag="VFL84_10405"
     CDS             complement(4722..>5339)
                     /locus_tag="VFL84_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609078.1"
                     /translation="TGGNENFAKLGVTILGRPALRAQLLNPRPAPGNGQVPPVAPPAA
                     LPVVMFDAPTTVYFNGETVQLVPLNPAHTDGDTAVKFERADVLMTGDVYRSAGFPAAA
                     LTNGGSVLGTINELDRLIALTGPNTKVVPGHGPVVDRAALVFHRDMAVTVRDRVAKAL
                     RDGKTADEIRASKPTAEFEERVGGPPNFIAQFVDGLITELSAQRR"
BASE COUNT          853 a         1772 c         1790 g          924 t
ORIGIN      
        1 cgcgcgcacg aattcagcat ccgcacggcg atgggcgccg gacggcgcga catcgttctt
       61 ctcgtgctcc gcgaaggcct catcgtcacc gtcgtcggca tactgatcgg catcgtcgcg
      121 gcgtttggct tgacgcggct catcgcgagc cggctatacg acgtcacgcc gacagaccct
      181 ctgacgatcg ttgccgtcgt ggtgttgctg acggtcatcg cgatgctcgc gtgcgtgatc
      241 cccgcacgtc atgccgccaa cgccgagccg ctccgggcgc tgcgcgtcga ctagcccgct
      301 cgatcgttgt aacggctaag aggcggcgtt tggacggccc ggttatgtgc gctggtgcgc
      361 ggtcccgcgg ctgcaaggct gctaccgtgc ccgcgagatc ccctcgtgag acaagcctgt
      421 aggacgtcgt aacgatggcc gaggacgctt tcaatcacgc cttgcgcggc aaagtcgcgc
      481 ccggctccga gctcgatgcc gagccgttcg gcagctcgcg ctcgttcaag cgtccgttgg
      541 tggtgtgcct cgtggggctc gtcgcgctcg gcgtcgcgct tcaatccctg ctgatctggc
      601 gcggcggcga ttcgacgcaa cgcgcgtcgc ttctcgccgc gcaagcgccg accttcgaga
      661 tcgaaatgcc gggcaccggg cgcaatctct tgctctcggt cgcggagttg cgctggtgct
      721 tgcgcgagga cttgcggatc gagatcttcg agcagcggct gtcgtacagc gagccgcagc
      781 ggttcggcgt catggtcgcg gactacaacc gccgctgcac gcgcttcaag taccgcgaca
      841 atgacctcga gcgcgcgcgc cgcgacatcg acggcgccag gaagtggatc gtcgaggaag
      901 cgttcgcaga agcatattcg acggctgcgg gcggggcgcc cgacaggctt gcggcgaacg
      961 gctactcgat actgaccaaa gacgtgcaac ggctgttgcg tgcggtcggt tacgatcccg
     1021 ggcccatcga cgggcactac ggcccgcgaa cccgggcagc cgtcgagtcg ttcgaggccg
     1081 aggtcggcag agatccgacg ggcggcatct cggaagcgct gcgccgggag ctgctcgacc
     1141 gggttcgcag cggcggattt gccgagtcgc ggctgctcgc cgcgacggcc gcggaggccg
     1201 cgacgatccg cgcgagctgt gccgccgcga acggcgtcgc ggcgtacaac cggtgcgtcg
     1261 agtccgagct cgcggcgctc gcgcagcggc ggccgcctcc ggcgcgggcc atgaccgcgg
     1321 cggagcgcga cacgatcgac gaggtctgtc tgcgcgccaa gctgcgcggc ggcgcggcag
     1381 gttacgaccg ttgcgtcgag gaacagatcg cggagctggc ggcgctcgag agcgagccga
     1441 gcgttgccgc gatgacgagg cgccaacgcg ccacggtcga ggatcggtgc gcgagcacgg
     1501 ggtccttcta cgggcccgcg gccttttatc gttgcgcgca agaacgcctc gcggaagtcg
     1561 ccgcggcagg ccggaacgct tcgctctagt ctcgtcacgc tccgacggca actctgcgct
     1621 accatccgca gagtcctgat tcgcccggag gcaagcgatg aaacggcgaa tgcgcatgtt
     1681 cgtcgtcgcg ttgacgctca gcgcgtcggc ctctatcgtg ttcgcccagt tttttcgccg
     1741 cggctactcg cccggcgtgg accagccgct cgtcggcgag ccgaaggagt ggacgtttgc
     1801 gcggctcgcg tacgacggca cgggcatgcg cgggcgcagc ggctggtcgg tcgactatcc
     1861 gtacgccgag attcatttct cggacgccgt cgaccggctg acgcgcatcg acgtccatcc
     1921 ggacggccac gtgatcagcc ccgactccga cgacctgttc aactacccgt ggctgtatgc
     1981 ggtcgaggtc ggatcctgga cgttcagcga ttcgcaggcg gcgcggatgc gcgagtatct
     2041 gctgcgcggc gggtttctca tggtcgacga ctttcacggc gagtacgagt ggcaggtctt
     2101 cgtcgaaggc atgcgcatga tctttccgga tcggccgatc gaggacatcc cggcgaccga
     2161 tgtcatctac tcgcagccgt atgagatcga gaagcgcgtg caggtgccgg gccccgatta
     2221 catgacgacc ggattgacct acgagcggtc cgacggcgtg acgccgcatt ggcgcggcat
     2281 ccgcgatgcc gacggccgat ggatggtcat gatcacgcac aacatcgact acggcgaagg
     2341 ttgggagcag gccgacaacc cgcgctaccc ggaagccttc acgcgtcaag cgtacgaggt
     2401 cgccgtcaac tacctgatct acacgatgac gcactgacac gcggtcaggg cgcgcacgag
     2461 tgagtcgccc tcaaggaatc gcgtgatgca cgaagtcgtc gggcggcggc gtgccccatt
     2521 gcgcgctgaa cccgatctcg accgggtgca agccggcgcc cgcgcgagcg ttcagcacgt
     2581 ctgaatcggt ccagtgctcg tggacgcggc gccacgggtc ttcccagtag tcgaagatct
     2641 gactgccgag caaatggcgg ccgatgcccc agacgtgccg gtacttcccg gcttgcttca
     2701 agtgctcgtg accgaccatg aggtcgtcga agccctgcac ctcgaacgac aagtgattca
     2761 gccccgtcgt cggcgattcg aagcagagca acgtgtgatg gtcgacgaaa gtttcgccgc
     2821 ggtcgcagcg gttgaaagac gcgacgatgt tgtccttcga gccttcgtag acttcgtccg
     2881 aaggcaagaa accgagcatc tcgcgatacc agcgaatctt gtccgctgcg ctcttcgcca
     2941 tgaggacggc gtgcgcgatg cgcttgacgc gcgacggccc ccgctcgacg cggaacagct
     3001 ctccggcgcg gcgcagcttg ttcgtgcccg tattcgtcga ctgctccgcg gccggcagcc
     3061 gcgggagcgt ctgcatcccg cagacgactt cgactcgata accgagcgga tccgttagac
     3121 tcacgcgctt gccgccgccg ggctcgtcga cgtgctcgac gccgcttgcg cccggcgcgc
     3181 gcgcgagcct ggcgagatcg tcttcgctct ccgcgaagaa gccgagcccc atgaacctcg
     3241 acggtcccaa gtgagtgacg tgaacgtggt gggacgggtc cgtgccgcgc atgtagagcg
     3301 cgtcgcgcgt gcgctcggtg cgcacgaggc caaatgccgt caggaactcc tcggcctcgt
     3361 ccaggtttgg ggattgcagc cgcccgtagg cgaggtcggt aactttgatg aagggcatgc
     3421 cagtcaactg tcgcgggggt ttggcggccg cacacacgcg cggaaagtaa ctcgccgccc
     3481 cgattctgta aaacccgcga acccgatcgc gattccgcgg ttccgatcgc gtcaccgcgc
     3541 tccggccaac acgaacttct gcgcgcgccg gttgttgacg atctccgccg tgtagaggtt
     3601 gcccttagag tcgaccgcca tgtggtggac gatgtcgaac tcgccggcgt tggcaccctt
     3661 catgccgacc acgccgattt gcgtcatcgt gtcgcgatcg aagatcacga cgcgcatcgg
     3721 ccccgagtcg acgacgtaga gaaactgctg cttcgcgtcg ggcgagaacg cgaagcccgc
     3781 cggaaccggc acgacgtcgc tgccttcgtt cgtcacgcgc acttgcttca ggaacttgcc
     3841 ctcgcgcgtg aacagctgga tgcggttgtt gatgcgatcg gcgagataaa cgacgccgtc
     3901 gttcgatacc ttgatcgcgt gcggcaagcc gaactcgggc gggccctcgg gcgtcgtctg
     3961 tggctgcggc gttggggcgt tcgccgcgaa cgtcgcgggc ggcgggttgc cgaacgcgcc
     4021 ccacatgcgc ttgaacttgc cggtctccga gtcgaacacg atgacgcgct tgttgccgta
     4081 gccgtccgcg gcgtaaacct ctttcgcgcg cgtgtcgacg aacaggtcgg tggcttggtg
     4141 cacgttctcc gtatcgagat tgcccgtgct cgcgccgctg cggccgatct gcatcacgag
     4201 ctcgcccgtc atcgtgaact tcatgatcat gtcgtcgctg acgccgggcg tggtctgccg
     4261 cggccagccc gcgcgcccgc cgatccagac gaagtcgttc gcgtcgacga agatgccgtg
     4321 ctcgcggccg agccacgccg cgccgttgct cggtccgccc cagcttcgca gcagtttgcc
     4381 ggcggaatcg aactcgagca ccggcggcgc cgcattcgcg cgcttgtcct cggggatcga
     4441 ctggggcacg tgcagcaccc agacgttgtc gttgcggtcg accgagatcg acgtcacctc
     4501 gccgagcacc cagtcgttcg ggatcgtcgg ccagctggga tccactttga actgaggcgc
     4561 gcgcggatcg gactgggcgt gcgcgctgcc aaccatgcac accgaagcgg ccagcatccc
     4621 cgccaaggac acaatagagc caagcgtcgt cttcatcgaa cccccctcaa aatggggaca
     4681 gatctatttt cggagaaaaa tagatccgtc cccggagcct gtcagcgccg ctgtgcgctg
     4741 agctccgtta tcaacccgtc gacgaattgc gcgatgaaat tcggcggccc gccgacgcgc
     4801 tcctcgaact ccgcagtcgg cttcgacgcg cgaatctcgt ccgcggtctt gccgtcgcgc
     4861 agcgccttcg cgacgcgatc gcgcacggtg acggccatgt cgcgatggaa aaccagcgcg
     4921 gctcggtcga cgacgggccc gtggcccggc acgaccttcg tgttcggccc ggttagagcg
     4981 atcagcctat cgagctcgtt gatcgtgccg agcacgctac cgccgttcgt gagggcggcc
     5041 gcgggaaagc cggccgagcg atagacgtcg ccggtcatga gcacgtcggc gcgctcgaat
     5101 ttcacggccg tgtcgccgtc cgtgtgcgcg gggttcagcg gcacgagctg cacggtctcg
     5161 ccgttgaagt aaaccgtcgt cggcgcatcg aacatgacga ccggcaaagc ggccggcggc
     5221 gcaacgggtg gcacttggcc gttgcccggc gccggcctcg ggttcaggag ctgcgcgcgc
     5281 aatgccggac gcccgaggat cgtgacgccg agcttcgcga agttctcgtt gccgcccgt
//
LOCUS       DASSNN010000560         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_600580, whole genome shotgun sequence.
ACCESSION   DASSNN010000560 DASSNN010000000
VERSION     DASSNN010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600580"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFL84_10410"
     CDS             <1..492
                     /locus_tag="VFL84_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HET7609079.1"
                     /translation="FSVEAMRTVEVAGERASSTAVRDALAKGDLARAAALLGRNYAIS
                     GHVGHGDKRGRGLGFPTANVALRRTPPLTGIFTVRVHGLPGGPCTGVASVGVRPTVKT
                     DGKPLLEVFMLDFDETIYGRRITVEFLHKLRDEARFPDVETLVRQMGRDVADAREYFA
                     AHG"
     gene            800..>2372
                     /locus_tag="VFL84_10415"
     CDS             800..>2372
                     /locus_tag="VFL84_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET7609080.1"
                     /translation="MPDDSRTDYKSTLNLPDTAFPMRGDLARREPGWVDTWRHARVYE
                     AIRAASRGRPRFILHDGPPYANGDIHIGHAVNKILKDTIVKSRALAGYDAPYVPGWDC
                     HGMPIEVQIEKTHGKNLSTAETQRLCRAYATEQIERQKADFQRLGVLGDWDHPYKTMD
                     FRNEADEIRTLGRLLQKGYLYRGLKPVNWCFDCQSALAEAEVEYEDRVDVAIDVGFPL
                     VDTDERTRLARAFGIDALPDGPVYAVIWTTTPWTIPANQALNVHPDVVYALVTTPRGS
                     LVLAKDLVTACLARYGLQGEVSATAAGAALEKIAFRHPFYDRASPVYLGDFVTLDQGT
                     GIVHSSPAYGIDDFLSSRRYGMPDDEILNPVQGDGRYAADLPYFGGLRIWDANPRIVD
                     KLKESHALFHAEKFPHSYMHCWRHKTPIIYRATTQWFAGMDDVPGFRGVKPAETLRTT
                     ALRGVDGTEFFPAWGKARLRGMIANRPDWTLSRQRQWGVPLPFFVDRETDELHPDTLE
                     LLELAATKVEAGGIET"
BASE COUNT          422 a          771 c          793 g          386 t
ORIGIN      
        1 ttcagcgtcg aggcgatgcg caccgtcgag gttgccggcg agcgtgcgtc gagcacggcg
       61 gtacgcgatg cgcttgccaa gggcgatctc gcgcgcgccg cggcgctgct cggacgcaat
      121 tacgcgatca gcggccacgt cgggcacggc gacaagcgtg gacgcggtct cggttttccg
      181 acggcgaacg tcgcgctgcg ccgcacgccg ccgctgaccg ggatctttac cgtgcgcgtg
      241 cacggcctgc cgggagggcc atgcaccggc gtcgcaagcg tcggcgtacg accgacggtg
      301 aagacggacg gcaagccgct tctggaagtg ttcatgctcg atttcgacga gacgatctac
      361 ggccggcgca tcaccgtcga gttcctgcac aagttgcgcg acgaggcgcg cttccccgac
      421 gtcgagacgc tcgtccggca aatggggcgc gacgtcgcgg atgcgcgcga gtacttcgcc
      481 gcgcacggct gacgtgaacc ccgcggcgcc cgaaaccaag ggcctgttca cactatgttc
      541 aggaatgcga acggacggca tgggtcgcca atcaaggcgc tcgacgaagc gaagactggc
      601 tgtctttgcg atgagagcaa cgcagagtgg cgacccatgc cgcccgttcc ctccgggttg
      661 ctgcgaaaac ggcactgcgc gtcgttacga aagctcgccg ggcgtccagc ccgccttcgc
      721 tttcacgtct cgcgcagagc cgttttcgca atgcaacgca ttcgcgaaca tagtgtgaac
      781 aggccctaaa agtgcatcca tgcccgacga ctcccgaacc gattacaagt cgacgctgaa
      841 ccttcccgac acggcgtttc cgatgcgcgg cgatctcgcg cgcagggagc cgggatgggt
      901 cgacacgtgg cgccacgccc gcgtgtacga ggcgattcgg gcggcctcgc gcggccggcc
      961 gcgcttcatc cttcacgatg ggccgccgta cgcgaacggc gacatccaca tcgggcacgc
     1021 agtcaacaag atcctgaagg acacgatcgt caagagccgc gcgctcgccg gctatgacgc
     1081 tccgtacgta ccgggatggg attgccacgg catgccgatc gaggtgcaga tcgagaagac
     1141 gcatggcaag aatttatcga ccgcggagac gcagcgcttg tgtcgtgcat acgcgaccga
     1201 gcagatcgag cgccagaagg ccgatttcca gcggctcggc gtgctgggtg actgggacca
     1261 tccgtacaag acgatggact tccgcaacga ggcggacgag attcgcacgc tcggccggct
     1321 gttgcagaag ggctacctgt atcgcggcct gaagcccgtg aactggtgct tcgattgcca
     1381 gagcgcgctc gccgaagcgg aagtcgagta cgaggatcgc gtcgatgtcg ccatcgacgt
     1441 cggctttccg cttgtggaca cggacgagcg gacgcggctc gcgcgcgcgt tcggcatcga
     1501 tgcgcttccc gacggaccgg tctacgccgt catctggacg acgacgccgt ggacgattcc
     1561 ggccaaccag gcgctgaacg tccatcccga cgtcgtctac gcgctggtga ccacgccgcg
     1621 gggatcgctg gtgctggcca aggacctcgt aaccgcgtgc ctcgcgcgct atggcctgca
     1681 aggcgaagtg tcggcaacgg ctgccggcgc ggcgctcgaa aagatcgcgt tccgccaccc
     1741 gttctacgac cgcgcgtcgc ctgtgtacct cggtgatttc gtgacgctcg accagggcac
     1801 cggcatcgtg cacagctcgc ccgcgtacgg catcgacgac ttcctgtcga gtcgccgcta
     1861 tggaatgcct gacgatgaaa tcctgaaccc ggtgcagggc gatggccggt acgctgccga
     1921 cctgccgtac ttcggcggct tgcggatatg ggacgcgaat ccgcgcatcg tcgacaagct
     1981 gaaggaatcg catgcgctgt tccatgcgga gaaatttccg cacagctaca tgcactgctg
     2041 gcggcacaag acgccgatca tctatcgcgc gacgacgcag tggttcgcgg gcatggacga
     2101 cgtgcccggc tttcgcggcg tcaagcctgc ggagacgctg cggacgacgg cgttgcgcgg
     2161 cgtcgacggc accgagttct tccccgcctg ggggaaggcg aggctgcgcg ggatgatcgc
     2221 caatcgcccg gactggacgc tgtcgcggca gcgccagtgg ggcgtgccgc tcccgttttt
     2281 cgtcgatcgc gaaaccgacg agctgcatcc cgacacgctc gagctgctcg agctcgccgc
     2341 aacgaaagtc gaggcgggcg gcatcgagac gt
//
LOCUS       DASSNN010000561         3361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1480746, whole genome shotgun sequence.
ACCESSION   DASSNN010000561 DASSNN010000000
VERSION     DASSNN010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480746"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFL84_10420"
     CDS             complement(<1..418)
                     /locus_tag="VFL84_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609081.1"
                     /translation="MHLFEGPTARSLRHVAFRVLVVWFAILGSGSPAWGQARSSCPGG
                     RDSVLTNGTILTMDEADRTVTTVRIRDGRFLTVGEAVARNEPCVQVVDLGGRTAIPGL
                     IDGHTHFVRTAQAPGPFIEGLEAARSIAELQAALAAA"
     gene            531..>3361
                     /locus_tag="VFL84_10425"
     CDS             531..>3361
                     /locus_tag="VFL84_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7609082.1"
                     /translation="MDRLSTLAFSTLLYGIAIAMKFTAWRHPAFRERLKEQNLVAQIR
                     VKQLPTGRYYVIKDGKVSSRSGIHPNPDVVVAVKSARLGFQIFTHPNDQLGRINAAKS
                     FSMTLDGPQDKAIWFMFTLESMMQAGWRYGVDMGNGETRYANMANGGPLYVYVKDGKI
                     VRMTPIEFDASDPQPWTIKAKGKEFTPPRKTSLAPHGQTVKSTVYSPDRILYPMKRVD
                     FDPNGERNPQNRGISGYERISWDEALDIVANEIKRQKTVHGNGAIACSHPSHHAWGNI
                     GYYLSALRKFENAVGMTEVHHNPDSWEGWYWGATHHWGGSMRVGQSETYGTVEDCLKE
                     AEMVVFWSSNPESNSGAYGALEGTIRRQWLKEAGIKLVHIDPYYNDSIQLLGGKWIAP
                     RPTSSPALALAIAYVWITEGTYDKQYIETRTHGFEKWRDYVLGKTDGIPKSPEWQEAE
                     TAVPASDVRALAREWASKKTYLGAGAWGNGHGGACRNATGIQWARTMVCLIAMQGLGK
                     PGVNMGNLQWGTPLDFNFYFPGYAEGGMSGDFSHTAMGVVLYQRMPQLPSINTNTQVI
                     PRMQLPEAIIDGKAEGYVWNGSSIEAQFQKITYPKPGFSPVHMLWKYGGSLIASMPDS
                     SRYINMFRHPNLECLVSQNIWLHGDTEFADIILPACTNFERYDISEWAGLGGYAHHGE
                     QQLNHRVIVFQHKCIEPLGESKSDFWIFQKICERLDLAAYFTEGVGELGWVRRQFEGS
                     DLPKHISWKEFIRKGYFVVPTEKEEQRAPLSWNWFYEGRKKDVPEPMPLPSDYSQGYL
                     NGIQTQTGKIEFECESLKRFDPNSEDRPPIAKYQRPAEFPSNEGYEDFPLQLISPHPR
                     FSFHTQMDGKDSFLNDIPDHRVLIDGYYYWVMRIHPDDAKARGIRDNDLVRLYNERGA
                     VICAAKLTHRMLKGVVHSYASSGVYDPVG"
BASE COUNT          689 a         1105 c         1055 g          512 t
ORIGIN      
        1 cggcggccgc gagcgccgct tggagctccg cgatggagcg cgcggcttcg aggccttcga
       61 tgaacggacc aggcgcctgc gccgtgcgca cgaaatgcgt gtggccgtcg atcaatcccg
      121 gaatcgccgt gcggccgccc aagtcgacga cctgcacgca cggctcgttg cgcgcgacgg
      181 cttctccgac cgtcagaaac cgtccgtcgc gtatgcggac ggtggtcacg gttcggtccg
      241 cctcatccat cgtgagaatc gtgccgttcg tgagaacgga gtcgcggccc ccggggcacg
      301 agctccgggc ttgcccccaa gccggcgagc cgctgccgag aatggcaaac cacacgacga
      361 gcacgcgaaa tgcgacgtgg cgcaacgagc gtgctgttgg cccttcaaat agatgcatgc
      421 gaaccccccg tttgcggcac cagccttacg attatcagtc gaaaccggta acctacggag
      481 cccgaaatac ctcgcggaag cgcgacaaca ataggggaag gctaggcctg atggacagac
      541 tcagcacgct ggcgttttcc acactgctat acggcatcgc gatcgcgatg aaattcaccg
      601 cgtggcgcca tcccgccttc cgcgagcggc tcaaagagca aaacctcgtc gctcagatcc
      661 gcgtcaagca gctgccgacc gggcgctact acgtcatcaa ggacggcaag gtgtcgtcgc
      721 gcagcggcat tcacccgaac cccgacgtcg tcgtcgccgt caagagcgca cgcctcggct
      781 tccagatctt cacgcatccg aacgaccagc tcggccggat caacgcggcg aagtctttca
      841 gcatgacgct cgacgggccg caggacaaag ccatctggtt catgttcacg ctcgaatcga
      901 tgatgcaggc cggctggcgg tacggcgtcg atatgggcaa cggcgagacg cgctacgcga
      961 acatggcgaa cggcggcccg ctctacgtct acgtcaaaga cggcaagatc gtgcgcatga
     1021 cgccgatcga gttcgacgcc agcgacccgc agccgtggac gatcaaagcg aagggcaaag
     1081 agttcacgcc gccgcgcaag acgtcgctcg cgccgcacgg gcagaccgtc aagtcgacgg
     1141 tgtattcgcc cgatcgaatc ctgtatccga tgaagcgcgt cgacttcgat ccgaacggcg
     1201 agcgcaatcc gcagaaccgc ggcatctcgg gctacgagcg catcagctgg gacgaagcgc
     1261 tcgacatcgt ggcgaacgag atcaagcggc agaaaaccgt gcacggcaac ggcgcgatcg
     1321 cgtgcagcca tccgtcgcac catgcgtggg gcaacatcgg ctattacctc tcggcgctgc
     1381 gcaagttcga gaacgccgtc ggcatgaccg aggtgcatca caaccccgac agctgggaag
     1441 gctggtactg gggcgcgacg caccactggg gcggctcgat gcgcgtcggc cagtcggaga
     1501 cctacggcac cgtcgaagac tgcctcaaag aggccgagat ggtcgtgttc tggtcgagca
     1561 accccgagtc gaacagcggc gcgtacggcg cgctcgaagg cacgattcgc cggcagtggc
     1621 tcaaagaggc cggcatcaag ctcgtgcaca tcgaccccta ctacaacgac tcgattcagc
     1681 tcttgggcgg caagtggatc gcgccgcggc cgacgtcgag cccggcgctc gcgctcgcga
     1741 tcgcctacgt ctggatcacc gagggcacgt acgacaagca gtacatcgag acgcgcacgc
     1801 acggcttcga aaagtggcgc gactacgtgc tcggcaagac cgacggcatc ccgaagtcgc
     1861 ccgagtggca ggaggccgag acggccgtgc cggccagcga cgtgcgcgcg ctcgcgcgcg
     1921 aatgggccag caagaagacc tacctcggcg ccggcgcctg gggcaacggc cacggcggcg
     1981 cgtgccgcaa cgcgaccggc atccagtggg cgcgcacgat ggtgtgcctg atcgcgatgc
     2041 agggactcgg caaacccggc gtcaacatgg gcaacctgca atggggcacg ccgctcgact
     2101 tcaacttcta tttcccgggg tacgccgaag gcggcatgtc gggcgatttc agccacacgg
     2161 cgatgggcgt cgtgctctac cagcgcatgc cgcagctgcc gtcgatcaac acgaacacgc
     2221 aagtcattcc gcgcatgcag ctgccggaag cgatcatcga cggcaaggcg gaaggctacg
     2281 tctggaacgg ctcgagcatc gaggcgcaat tccagaagat cacgtatccg aagcccgggt
     2341 tctcgcccgt gcacatgctc tggaagtacg gcggctcgct gatcgcgtcg atgccggaca
     2401 gcagccgcta catcaacatg ttccgccacc cgaacctcga gtgcctcgtg agccagaaca
     2461 tctggctgca cggcgacacg gagttcgcgg acatcatcct gccggcctgc acgaacttcg
     2521 agcgctacga catcagcgaa tgggcgggct tgggcggcta cgcgcaccac ggcgagcagc
     2581 agctcaatca ccgcgtcatc gtgtttcagc acaagtgcat cgagccgctc ggcgagtcga
     2641 agtcggattt ctggatcttc cagaagatct gcgagcgcct cgacctcgcc gcctacttca
     2701 cggaaggcgt cggcgagctc ggctgggtgc gcaggcagtt cgagggatcg gacttgccga
     2761 agcacatctc gtggaaggag ttcatccgca agggctactt cgtcgttccg accgaaaaag
     2821 aagagcagcg cgcgccgctc tcgtggaact ggttctacga gggccgcaag aaagacgtcc
     2881 ccgagccgat gccgctgccg tccgattact cgcaaggcta tttgaacggc atccagacgc
     2941 agaccggcaa gatcgagttc gagtgcgaga gcctgaagcg cttcgatccg aatagcgagg
     3001 accggccgcc gatcgcgaag tatcagcgtc ccgcggagtt cccgagcaac gaaggctacg
     3061 aggactttcc gctgcagctg atctcaccgc acccgcggtt ctcgttccac acgcagatgg
     3121 acggcaaaga cagcttcttg aacgacatcc ccgatcatcg cgtgctcatt gacggctact
     3181 actactgggt catgcgcatc caccccgacg acgcgaaagc gcgcggcatc cgcgacaacg
     3241 acttggtgcg cctctataac gagcgcggcg ccgtcatctg cgccgcgaag ctcacgcacc
     3301 gcatgctgaa aggcgtcgtg cactcgtacg cgtcgtcggg cgtctacgac cccgtcggcg
     3361 a
//
LOCUS       DASSNN010000562         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_837747, whole genome shotgun sequence.
ACCESSION   DASSNN010000562 DASSNN010000000
VERSION     DASSNN010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837747"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFL84_10430"
     CDS             complement(<1..275)
                     /locus_tag="VFL84_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609083.1"
                     /translation="MRVAVVIAAAVAAAALEAQPARDLSGHWDRASPLESFANTPGGT
                     RDNAQAGAREAPFTPAGRAAFEANKPGYGPRRAMARNDPMGRCEPLGL"
     gene            complement(275..661)
                     /locus_tag="VFL84_10435"
     CDS             complement(275..661)
                     /locus_tag="VFL84_10435"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609084.1"
                     /translation="MRPVILRRGVRAAAALWLLAAWPAAAHHGGDVEWAEKAVGPITG
                     TATKFAFQFPHVFFEMTVEENGAPTPWTITTRWTPTILRDHGWTRESIKPGDKVTVTY
                     LPHIEREHIGQMQTIDVNGQSLPLSF"
     gene            755..>2680
                     /locus_tag="VFL84_10440"
     CDS             755..>2680
                     /locus_tag="VFL84_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7609085.1"
                     /translation="MRVLLLTPPMTQLNTPYPATPYLTGFLRAHGGDLDLEVSQADAS
                     LALFLRLFSAPWLTRMAEVLEQRARAARRGSSPPPSIAQFLSDAPRYAATVEPAVRFL
                     QGRDPSLALRIVSRTFLPEGPRFAPLAAAATAGDDPLSAAFGALGTTDQARYLASLYV
                     DDLADAWRDGIDPNFELARYGERLAASAPTFEPLQAALLGAPTLVDEALDQIASELAA
                     QHRPDVVGMSVPFPGNVYGAFRMARVMRAAAPHAKLVLGGGWVNTELRALKEPRVFDY
                     FDYVTLDDGERPLLNLLSRLNGRAAPLVRTYVRRGDEVALETDATQHDFPARDTGIPT
                     YDGLPLGDYVSVLEMLNPMHRLWSDGRWNKITLAHGCYWKKCSFCDVSLDYIGRYDRP
                     ETDVVMARIRALIAETGQTGFHLVDEAAPPAGMRALAKRLLEEKLSITWWGNIRFEKT
                     FTRELCGLLAESGCVAVSGGLEVASDRLLELMKKGVTVAQVARVTRAFTDAGIMVHAY
                     LMYGFPSETVQETIDALERVRQLFELGCIQSAYWHRFSATAHSPVGLEPERYGITLRP
                     PPNITFAHNDLEFDDPVGTDHDFLGAGLRKALYNYMLGVGLDADVRAWFEAPLPARRA
                     RGQRPRRRAPRVPKTTVP"
BASE COUNT          390 a          984 c          897 g          409 t
ORIGIN      
        1 aggccgagcg gctcgcagcg gcccatcgga tcgttgcgcg ccatcgcgcg gcgcggcccg
       61 tagccgggct tgttggcctc gaacgccgcg cggccggccg gcgtgaacgg cgcctcgcgc
      121 gcgccggctt gggcgttgtc gcgcgtgccg cccggcgtgt tcgcgaacga ctcgagcggc
      181 gacgcacggt cccagtgccc cgacagatcg cgggcgggct gcgcctcgag tgccgccgca
      241 gcgactgccg ccgcgatgac gacggcgacg cgcatcagaa cgacaacggc agcgactggc
      301 cgttgacgtc gatcgtctgc atctgcccga tgtgctctct ctcgatgtgc ggcagatacg
      361 tgaccgtgac tttgtcgccg ggcttgatcg actcgcgcgt ccagccgtgg tcgcgcagga
      421 tcgtcggcgt ccagcgcgtc gtgatcgtcc acggcgtcgg cgcgccgttc tcctcgacgg
      481 tcatctcgaa gaacacgtgc ggaaactgga acgcgaactt cgtcgccgtg cccgtgatgg
      541 gcccgaccgc cttctcggcc cattcgacgt cgccgccgtg atgggccgcc gccggccatg
      601 ccgcgagcag ccacaacgcg gccgcggcac ggacgccgcg gcgcaggatc accggtcgca
      661 ttgctttccc cccgggccgc aaagtgcagg agccatcttg cggccgcacc gcccggtttt
      721 caaacggccg aggcgcgggc taacctcacg cggcatgcgc gtactcttgc tgacaccgcc
      781 gatgacgcag ctcaacacgc cgtacccggc gacgccgtac ttgaccggct ttctgcgcgc
      841 gcacggcggc gacctcgatc tcgaagtgag ccaggccgac gcctcgttgg ccctgttcct
      901 gcgactgttc tcggccccgt ggctcacgcg catggccgaa gtgctggagc aacgcgcgcg
      961 tgccgcacgg cgaggctcgt cgccgccgcc gtcgatcgcg cagttcttga gcgacgctcc
     1021 gcgctacgcg gcgaccgtcg agcccgccgt ccggttcctg caagggcgcg acccgagcct
     1081 cgcgctgcgc atcgtctcgc gcacgttcct gccggaagga ccgcgcttcg cgccgctcgc
     1141 ggcagccgcg acggccggcg acgacccttt gagcgcggca ttcggcgcgc tcggcaccac
     1201 ggaccaggcg cgctatctcg cgagcctcta cgtcgacgac ttggccgacg cgtggcgcga
     1261 cggcatcgat cccaacttcg agctcgcgcg ctacggcgag cggctcgcgg cgagcgcgcc
     1321 gaccttcgag ccgctccagg cggcgcttct gggcgcgccg acgctcgtcg acgaggcgct
     1381 cgaccagatc gcgagcgagc tcgccgcaca gcaccgcccg gacgtcgtcg gcatgtcggt
     1441 gccgtttccc ggcaacgtct atggcgcatt ccgcatggcg cgcgtcatgc gcgccgccgc
     1501 gccgcacgcg aagctcgtgc tcggcggcgg ctgggtcaac acggagctgc gcgcgctcaa
     1561 ggagccgcgc gtcttcgact acttcgacta cgtcacgctc gacgacggcg agcggccgct
     1621 cctcaacttg ttgtcgcgct tgaacggccg agccgcgccg ctagtgcgca cgtacgttcg
     1681 ccgcggcgat gaggtcgcgc tcgagaccga cgcgacgcaa cacgacttcc ccgcgcgcga
     1741 cacgggcatc ccgacctacg acggcttgcc gctcggcgac tacgtatccg tgctcgagat
     1801 gctgaacccg atgcatcgcc tctggtcgga cggccgctgg aacaagatca cgcttgcgca
     1861 cggctgctac tggaagaagt gcagcttctg cgacgtgtcg ctcgactaca tcggccgtta
     1921 cgaccggccc gaaaccgacg tcgtcatggc gcgcattcgc gcgctgatcg cggagacggg
     1981 gcaaaccggc tttcatctcg tcgacgaagc cgcaccgccc gccggcatgc gcgcgctcgc
     2041 gaagcggctg ctcgaggaaa agctcagcat cacgtggtgg ggcaacatcc gcttcgagaa
     2101 gacgttcacg cgcgagctat gcggcttgct cgccgagtcc ggctgcgtcg ccgtcagcgg
     2161 cggcctcgag gtcgcgtcgg accggctgct cgagctcatg aagaaaggag tcaccgtcgc
     2221 gcaagtcgcg cgcgtcacgc gcgcgttcac ggacgccggc atcatggtgc acgcgtattt
     2281 gatgtacggc ttcccgagcg agaccgtgca agagacgatc gacgcgctcg agcgcgtgcg
     2341 gcaattgttc gagctcggct gcatccagtc ggcgtactgg caccggttca gcgcgacggc
     2401 gcactcgccc gtcgggctcg agcccgagcg ctacggcatc acgctgcggc cgccgccgaa
     2461 catcacgttc gcgcacaacg atctcgagtt cgacgacccc gtcggaacgg atcacgactt
     2521 cctcggcgcc ggcctgcgca aggcgctcta caactacatg ctcggcgtcg ggctcgatgc
     2581 cgacgttcgt gcttggttcg aagcacccct gcctgcgcgc cgcgctagag gccaacgccc
     2641 gcggcggcgc gcgccacgcg tgcccaagac gacggtgccg
//
LOCUS       DASSNN010000563         3215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_685817, whole genome shotgun sequence.
ACCESSION   DASSNN010000563 DASSNN010000000
VERSION     DASSNN010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685817"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /gene="guaA"
                     /locus_tag="VFL84_10445"
     CDS             complement(<1..1028)
                     /gene="guaA"
                     /locus_tag="VFL84_10445"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET7609086.1"
                     /translation="MTKPVTALAEHKDAHAERILVVDYGGQYTQLIARRVREVGVYSE
                     ILPWDASPEAIAEFAPKGIILSGGPESVHEVGSPQISDALLSAGAPVLGICYGMQALA
                     AKLGGVVAPSDHREYGYAEVDLTGSDPLLAKLGARLKVWMSHGDRVERLPKGFAPTAK
                     SSNSPFAAMADVERKIYALQFHPEVTHTQHGLEIIRRFVLDICGCAPVWTAKNIVAEH
                     IAAIRAKVGRDRVLLGLSGGVDSSVVAALLHEAIGDQLVCVFVDTGLLRLNEGDQVMA
                     VFAKHLGANVIRVDAEKTFLAALAGVDDPEQKRKIIGRLFVEIFEEEAAKLSNVKWLA
                     QGTIYPDV"
     gene            complement(1028..2503)
                     /gene="guaB"
                     /locus_tag="VFL84_10450"
     CDS             complement(1028..2503)
                     /gene="guaB"
                     /locus_tag="VFL84_10450"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET7609087.1"
                     /translation="MRIIHEALTFDDVLLEPAYSEVLPREVKLRTQLTRSLSLNIPII
                     SAAMDTVTEARLAITIAQEGGIGIIHKNMSPKDQARQVQQVKKFESGVIHDPITVTPD
                     TTIRDVIALTRAKNISGVPVVEGENLVGIVTHRDLRFKTQRDAPVSAVMTPKDRLVTV
                     PEGAEKEKVLGLLHKHRIEKVLVVDKDKGFKLRGMITVKDIQKAHDYPLACKDERGAL
                     RVGAAVGTGGDTDERVALLVEAGVDVVIVDTAHGHSRGVIERVRKIKREHRELQLIAG
                     NVATAEGARALVEAGADGVKVGIGPGSICTTRIVTGVGVPQITAVTTVAEALKGTDVP
                     CISDGGIRYSGDIAKALAAGAHSTMIGGLFAGTEESPGDVELYQGRSYKSYRGMGSLG
                     AMTQKHGSSDRYFQDTTEELEKLVPEGIEGRVPYKGSIVAIVQQLVGGLRAAMGYTGC
                     RDITELRTKTKFVRITGAGRRESHVHDVTITKEAPNYQRSE"
     gene            2603..>3215
                     /gene="xseA"
                     /locus_tag="VFL84_10455"
     CDS             2603..>3215
                     /gene="xseA"
                     /locus_tag="VFL84_10455"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET7609088.1"
                     /translation="MQPGDSLQLPFAERRILSVASLNREARRIVESGLGTVWVEGEIS
                     NLSRPSSGHLYWSLKDAEAQVRCAMFRLATRGLGFELANGQQVLVRARASLYEARGEY
                     QLIVDYVEEAGEGILRRKFEELKRRLAAEGLFEPARKREIPKLPRRIGVVTSPTGAAL
                     RDVLIALRRRFPITAVLIYPTQVQGLGSAEEIARTLALADRRAE"
BASE COUNT          485 a         1073 c         1080 g          577 t
ORIGIN      
        1 atcacgtcgg ggtagatcgt gccctgcgcc agccacttca cattgctcag cttcgcggct
       61 tcttcctcga agatctccac gaacaggcgg ccgatgatct tgcgcttctg ctcgggatcg
      121 tcaactccgg cgagagcggc caagaatgtc ttctcggcgt cgacgcgaat cacgttcgcg
      181 cccaagtgtt tcgcgaacac ggccatgacc tggtcgcctt cgttcaagcg cagcaggcct
      241 gtgtcgacga acacgcagac gagctgatcg ccgatcgcct cgtgcagcag cgcggcgacg
      301 accgacgagt cgacgccgcc cgaaagcccg agcagcacgc ggtcgcggcc gaccttcgcg
      361 cgaatagccg cgatgtgctc ggcgacgatg ttcttcgccg tccaaacggg tgcgcagccg
      421 cagatgtcga gcacgaagcg gcgaatgatc tcgaggccat gctgcgtgtg cgtgacctcg
      481 ggatggaact gcagcgcata gatcttgcgc tcgacgtcgg ccatcgccgc gaacggagaa
      541 ttcgacgact tggccgtagg ggcgaagcct ttcggcaagc gctcgacgcg atcgccgtgg
      601 ctcatccaga ctttcaggcg cgcgccgagc ttcgcgagca gcggatcgct gcccgtcaga
      661 tccacctcgg cgtagccgta ctcgcgatga tcggaaggcg cgacgacgcc gccgagcttc
      721 gccgcgagcg cctgcatgcc gtagcaaatg ccgagcacgg gcgcgccggc ggaaagcagc
      781 gcgtcgctga tctgcggcga gcccacttcg tgcaccgact cgggcccacc cgacaggatg
      841 attcccttcg gcgcgaattc ggcgatggcc tccggcgagg cgtcccacgg caggatctcg
      901 ctgtagacgc cgacctcgcg cacgcgccgc gcgatgagct gcgtgtactg gccgccgtag
      961 tcgacgacga ggatccgctc ggcgtgcgcg tccttgtgct cggcaagcgc tgtgacgggc
     1021 ttcgtcatca ctctgagcgt tggtagttcg gcgcttcttt cgtgatcgtg acgtcgtgga
     1081 cgtggctctc gcggcgcccg gcgcccgtga tgcgcacgaa cttggtcttc gtccgcagct
     1141 cggtgatgtc gcggcaaccc gtataaccca tcgcggcgcg taagcccccg acgagctgct
     1201 gcacgatcgc gacgatgctg cctttgtacg gcacgcggcc ctcgatgcct tcgggtacga
     1261 gcttctcgag ctcttccgtc gtgtcctgga agtagcggtc ggacgagccg tgtttctgcg
     1321 tcatcgcgcc gagcgagccc atgccgcggt acgacttgta cgaccgccct tgatagagct
     1381 cgacgtcgcc gggcgactcc tcggtgccgg cgaacaaccc gccgatcatc gtcgaatgcg
     1441 cgccggccgc gagcgccttg gcgatgtcgc ccgagtagcg gatgccgccg tccgagatgc
     1501 acggcacgtc ggtgcccttg agcgcctcgg cgacggtcgt cacggccgtg atctgcggaa
     1561 cgccgacgcc cgtcacgatg cgcgtcgtgc agatcgagcc cgggccgatg ccgaccttga
     1621 cgccgtcggc gccggcctcg acgagcgcgc gcgcgccctc cgccgtcgcg acgttgcctg
     1681 cgatgagctg cagctcgcgg tgctcgcgct tgatcttgcg gactctctcg atgacgccgc
     1741 gcgagtggcc gtgcgcggtg tcgacgatga cgacgtcgac gccggcctcg acgagcagcg
     1801 cgacgcgctc gtcggtatcg ccgccggtgc cgacggccgc gccgacgcgc aacgcgccgc
     1861 gttcgtcctt gcacgcgagc ggatagtcgt gcgccttctg gatgtccttc acggtgatca
     1921 tgccgcgaag cttgaagccc ttgtccttgt cgacgacgag caccttctcg atgcggtgct
     1981 tgtgcaagag cccgagcacc ttctctttct cggcgccttc ggggacggtg acgagccggt
     2041 cctttggcgt catgaccgcg gagaccggcg cgtcgcgctg cgtcttgaac cgaagatcgc
     2101 ggtgcgtgac gatgccgacc aagttctcgc cctcgacgac cggaacgccg gagatgttct
     2161 tcgcgcgcgt cagcgcgatg acgtcgcgga tcgtcgtgtc cggcgtgacc gtgatcgggt
     2221 cgtggatgac gccgctctcg aacttcttga cctgctggac ttggcgcgcc tggtccttcg
     2281 gcgacatgtt cttgtggatg atgccgatgc cgccttcctg cgcgatcgtg atcgcgaggc
     2341 gcgcctcggt cacggtgtcc atcgcggccg agatgatcgg aatgttcagg ctcaaggagc
     2401 gcgtcagctg cgtcctgagc ttgacctcgc gcggcaaaac ctcggagtaa gcgggctcta
     2461 agaggacgtc atcgaaggtg agcgcctcgt gaatgatccg catagcggag ctcccgggga
     2521 atttgagccg cgcagtttac tggctcgacc ggccacgagc aaatcgccgg agctgccccc
     2581 gaagtcgttg tatcatgccg ccatgcagcc cggcgattcg ctccaactgc cgttcgcgga
     2641 gcgacggatt ctctcggtcg cgagtttgaa tcgcgaggcg cggcgcatcg tcgagtccgg
     2701 cctcggcacc gtctgggtcg agggcgagat ctcgaatctg tcgcgccctt cgtcggggca
     2761 tctctactgg tcgctcaagg atgccgaggc gcaggtgcgc tgcgcgatgt tccgtctcgc
     2821 gacgcggggc ttgggcttcg agctcgcgaa cggccaacaa gtcctcgtcc gagctcgcgc
     2881 gagcctctac gaggcgcgcg gcgaatatca gctcatcgtc gactacgtcg aggaagcggg
     2941 cgaaggcatc ctgcggcgca agttcgagga gctgaagcgg cggctcgcgg ccgagggcct
     3001 gttcgagccc gcgcgcaagc gcgagatacc gaagctgccg cggcgaatcg gcgtcgtgac
     3061 gtcgccgacc ggcgcggcgc tgcgcgacgt cctgatcgcg ttgcggcgcc gtttcccgat
     3121 cacggccgtt ctgatctatc cgacgcaggt gcagggcttg gggtcggccg aggagatcgc
     3181 gcgcacgctc gcgctcgccg atcggcgcgc cgagt
//
LOCUS       DASSNN010000564         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1844542, whole genome shotgun sequence.
ACCESSION   DASSNN010000564 DASSNN010000000
VERSION     DASSNN010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1844542"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFL84_10460"
     CDS             complement(<1..356)
                     /locus_tag="VFL84_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7609089.1"
                     /translation="MTHAIRFDSFGGPEVLQYREVEVPAPREGEARVAHRAIGVNFID
                     VYHRTGLYPLTLPSGLGGEGAGVVTAVGPGVRHVKAGDRVAYTAPPPLGSYSDERVVA
                     ARCLVKLPDSIGDETAA"
     gene            431..1648
                     /locus_tag="VFL84_10465"
     CDS             431..1648
                     /locus_tag="VFL84_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020795244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7609090.1"
                     /translation="MSEHLLIVGGGQAAAQAVQSLRQQNFAGAITLLCDEPYPPYQRP
                     PLSKIYFAGELPRERLLLRPAAFYAEKGVTLELETRVEEIEPAPRRVRLRDGRTIAYD
                     RLLLATGSRARVCPVPGADLPGVHYLRTIADVDAIIAALEPGARVLLVGAGYIGLEVA
                     ASARQRGFDVTVLEAAGRVMSRTVSAEVSAFYEACHRAAGVAIHCGAGVKAFRGSSRV
                     TAVETSDGRSFACDIAIVGIGIQPNVELAANAGLPCDNGIVVDELARTADPHIVAAGD
                     CTNHPHPLLERRVRLESVPNAIHQAKVAAATLLGSPTPYSEVPWFWSDQYDLKLQIAG
                     LSTGYDEVVLRGDMGKRSFAAFYLREGQLLAVDAVNSPREFIAGKKLVANRARIAPDV
                     LSDANVDLTTVGG"
     gene            complement(1651..>2084)
                     /locus_tag="VFL84_10470"
     CDS             complement(1651..>2084)
                     /locus_tag="VFL84_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609091.1"
                     /translation="LGLVLNDGSNQPAALASRLLSTSPDTDLLLNGVLVSDDWLVTIP
                     GEGSFYVHADSNAWPFLKEKLVPVWYLDQPWTGPAEYWPTVGPGADDSADVVGLTGSF
                     RGSEGSAVERYEITALDPARNVAQVKGELHLNLPGPQVAAQ"
BASE COUNT          326 a          718 c          712 g          328 t
ORIGIN      
        1 gcggccgtct cgtcgccgat cgaatccgga agcttgacga ggcagcgcgc cgcgacgacg
       61 cgttcgtcgg agtacgagcc gagcggcgga ggcgccgtgt acgcgacgcg gtcgcccgct
      121 ttcacgtgcc ggacacccgg cccgaccgcc gtcacgacgc ccgcgccttc gccgccgagc
      181 ccgctcggca acgtgagcgg atagagcccg gtgcggtgat agacgtcgat gaagttcacg
      241 ccgatcgcgc gatgcgcgac gcgcgcttcg ccctcgcgcg gcgccggaac ctcgacctcg
      301 cggtactgaa ggacctccgg cccgccgaac gaatcgaaac ggatggcgtg tgtcatgaca
      361 tctaatatcg ggcggcgctg ctagaatcgc cgccctgttt cggccctgtt tcaataaaat
      421 acagcattcg atgagcgagc atctcctgat cgtgggcggc ggccaggcgg ccgcgcaagc
      481 cgtgcaatcg ctgcgccagc agaacttcgc gggcgcgata acgctgctct gcgacgagcc
      541 ttatccgccg tatcagcggc cgccgctgtc gaaaatatat ttcgcgggcg agctaccgcg
      601 cgagcgcttg ctgcttcggc cggccgcgtt ctacgcggag aaaggtgtca ccctcgagct
      661 cgagacgcga gtcgaggaaa tcgagcccgc gccgcgccgc gtgcgcctcc gcgacggacg
      721 cacgatcgcg tacgaccgcc tgctgctcgc gacgggcagc cgtgcgcgcg tttgtcctgt
      781 gccaggcgcg gacctgccgg gcgtccacta cctgcgcacg atcgcggacg tcgacgcgat
      841 catcgcggcg ctcgagcccg gcgcgcgcgt gctgctcgtc ggcgccggct acatcgggct
      901 cgaggttgcc gcgtccgcgc ggcaacgcgg cttcgacgtc acggttctcg aagcggccgg
      961 gcgcgtcatg tcgcgcacgg tcagcgcgga ggtgtccgcg ttctacgaag cgtgtcatcg
     1021 tgccgccggc gtcgcgattc actgcggcgc gggcgtcaag gcgttccgtg ggtcctcgcg
     1081 cgtcaccgcg gtcgagacga gcgacggccg cagcttcgct tgcgacatcg cgatcgtcgg
     1141 catcggcatc cagccgaacg tcgagctcgc ggcgaacgcc ggcctgccgt gcgacaacgg
     1201 catcgtcgtc gacgagctcg cgcggacggc cgatccgcat atcgtcgccg ccggcgactg
     1261 cacgaatcat ccgcatccgc tgctcgagcg gcgcgtgcgt ctcgaatccg tgccgaacgc
     1321 gatccaccag gccaaggttg cggctgcgac tctgctcgga tctccgacgc cgtactcgga
     1381 ggtgccgtgg ttctggtccg atcagtacga cttgaagctg cagatcgcgg gcttgtcgac
     1441 cggctatgac gaggtcgtgc tgcgcggcga catgggaaag cgcagcttcg ccgcgttcta
     1501 tctgagagag gggcagctcc tcgccgtcga cgccgtcaac agcccgcgcg agttcatcgc
     1561 ggggaagaag ctcgtcgcga atcgcgcgcg catcgcgccc gacgtgctaa gcgacgcgaa
     1621 cgtggacctg acgacggtcg gaggttgaac ctactgcgcc gcgacctgcg gcccgggcag
     1681 attcaagtgc agctcgccct tcacctgcgc gacgttgcgc gccggatcga gcgcggtgat
     1741 ctcgtagcgc tcgacggcgc tgccttcgct gccgcggaac gagcccgtga gaccgacgac
     1801 gtcggcgctg tcgtccgcgc cggggccgac ggtcggccag tactcggccg ggcccgtcca
     1861 cggctgatcg aggtaccaga ccggcacgag cttctctttc aaaaacggcc aggcgttcga
     1921 atcggcgtgc acgtagaagc tgccttcgcc ggggatcgtg acgagccagt cgtcgctgac
     1981 gagcacgccg ttcaacaaca gatccgtgtc gggcgacgtc gacaacagcc ggctcgcgag
     2041 agccgccggt tgattcgagc cgtcgttgag cacgaggccg agca
//
LOCUS       DASSNN010000565         6286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_685914, whole genome shotgun sequence.
ACCESSION   DASSNN010000565 DASSNN010000000
VERSION     DASSNN010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685914"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /locus_tag="VFL84_10475"
     CDS             <1..1838
                     /locus_tag="VFL84_10475"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET7609092.1"
                     /translation="LNLGIAAAAAALARMHAPARSAAVEAPGAPRAGNVAAVYAVTAL
                     SGMTALAAEVLWTRHLSLLLGGTVYTFALIVAVLLLGLGVGSATGAVAGKRVDPRIAL
                     ASCQALLAIAMAAAGYALARSLPFWPIDVTLPTTAAVALQLDLLRVACVVLPAALLWG
                     ASFPLALAAAIRAGEEPRRAVGRLYAANTAGAIVGALATTFVLIVVVGSQRAAQLMIL
                     ASVAGAVVLLVRYHGFTSRRLQRAAGIAAACAAAAVAVPALPPELVAYGRFLPTRGLN
                     ANVVYVGEGVTASIAVTKEPNGTLTYHNAGKTQASTYPQDMRLQRMLGHLTTLVPAEP
                     RNVLVIGLGAGITAGAVSLDPAVERVVVAELEPLVPIVAAGFFDVHNYYVVTNDKVEI
                     RIDDGRHALATTAERFDAITSDPLDPWVKGAAALYTREFWQLCKERLNDGGVVTVFVP
                     LYETTEDAVRSELETFFDVFPNGAVFANTVDGMGYDVVLVGRNGDEPIDVAAVTQRIE
                     SGDYARVAASLRAVGFDSALDLLSTFTADAASLEPWLAGAKITTDRNLRLQYLAGEGL
                     NVLAAPRIFAAMTASAPGVPPKLFTGTPAQLEELRQRLAARQGRY"
     gene            complement(1871..4042)
                     /locus_tag="VFL84_10480"
     CDS             complement(1871..4042)
                     /locus_tag="VFL84_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET7609093.1"
                     /translation="MSAEPLHPSDQYYIVSTSARTDDRTRVMKHGDTFAVFDRFGDIE
                     QFGAGELGVYDHDTRILSRLALRLDGHRPLLLSSTIKDDNAVLAVDLMNPDLKRGDEL
                     IPRGTVHVDRSRVLLDGACHERIRVHNYGDSPVEIAMSIECGADFADIFEVRGTLRAR
                     RGRQLPARVREAELELGYEGLDGRVRRTRLEFDPPPTRLSDGRAEYALKLPPRGEAMC
                     QLVVSGERAGSRAALPTTSYEDAARKVSDILARARADEPQLRTSSEPFNDWLSRSLAD
                     LHMMRTQTPYGPYPYAGVPWFSTPFGRDGIITALECLWFNPGVAKGVLKYLAATQAEF
                     DRPERDAQPGKILHETRSGEMAATGEVPFERYYGSVDATPLFVMLAGAYYERTGDLAA
                     LEALWPNVEHALDWIDRYGDLDADGFVEYARRTESGLVQQGWKDSRDSVFHRDGTFAE
                     APIALCEVQGYVYAAKRSAASVAHALGDFARARELDDAAQALRQRFEEVFWCDDLSTY
                     ALALDGRKRLCRVRASNAGHCLYAGIVGDERAERLASTLLCDASYSGWGIRTLAQTEP
                     HYNPMSYHNGSIWPHDNALIAAGLARYGHKAEALRVLTGLFDASQFFDLHRLPELFCG
                     FARRPDESPTQYPVSCAPQSWASASVLMLLQACLGLEVRGAERKVVFANPVLPEFLRE
                     VHIDALRVGDASVDLYLVRHREDVGINVVRREGRVNVVTMK"
     gene            complement(4042..5094)
                     /locus_tag="VFL84_10485"
     CDS             complement(4042..5094)
                     /locus_tag="VFL84_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7609094.1"
                     /translation="MRIAQVAPLFESVPPKYYGGTERVVSYLTEELVSQGHEVTLFAS
                     GDSITEAKLVASSRRSLRLDKNCVDQMAHHVLMLERVVQRASSFDVIHYHVDYLHFPH
                     SRRHKTPHLTTLHGRLDIPDLQPLYREFRDMPVVSISGAQREPLPFANWIGTVHHGLP
                     PDLYRFHAEPGDYLAFIGRVAPEKRVDRAIEIAKRVGVPLRIAAKVDAIDREYFENVV
                     EPLLRDPLIEYLGEIGEGEKEEFFGNARLLLFPIDWPEPFGLVMIEALACGTPVIAFR
                     GGSVPEVLDDGVTGFIVDDLDDAVAAIRRIGEISRRRCREVFEDRFAASRMASDYIEL
                     YSALLEAPQRRMTGTS"
     gene            complement(5190..6062)
                     /locus_tag="VFL84_10490"
     CDS             complement(5190..6062)
                     /locus_tag="VFL84_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HET7609095.1"
                     /translation="MFAAKEHSLLVPFDGTFDARKAPTAPPKGKDKNGWKERLEDETK
                     TLGEAQERLYADGRYPVLIVLQALDAAGKDGTIRHVFTGVNPAGLRVAAFKQPTSLEL
                     AHDFLWRTTPHLPERGHIAIFNRSYYEEVLVVRVHPEFLAAQRLPDEPSPKLWANRLR
                     AIADHERYLAEQGMIILKFWLNVSKAEQRERFLERIDEPKKNWKFNPGDLDERDRWDD
                     YVAAYEECFRVTSRPWAPWYAIPSDDKHYQRWQVAKLINSALDGLDLRFPQPDEKTLA
                     ALTKAKARLLAQSD"
BASE COUNT          996 a         2115 c         2135 g         1040 t
ORIGIN      
        1 cgctgaatct cggcatcgct gcagcggccg cagcgctcgc gcggatgcac gcgccggcgc
       61 gctcggccgc ggtcgaagcg cccggcgcgc cgcgcgccgg taacgtggcc gccgtctacg
      121 ccgtcacggc gctgtcgggc atgacggcgc tcgctgccga ggtgctgtgg actcggcatc
      181 tgtcgctgct gctcggcggc accgtctata cgttcgcgct gatcgtcgcc gtattgctgc
      241 tcggcctggg ggtcggcagc gcgaccggcg ccgtcgcggg caagcgcgtc gacccgcgca
      301 tcgcgctcgc gtcgtgccag gcgctgctcg cgatcgcgat ggccgccgcg ggatatgcgc
      361 tcgcccggtc gctgccgttc tggccgatcg acgtcacgct gccgacgacg gcggccgtcg
      421 cgctgcagct cgacttgctg cgagtcgcgt gcgtcgtgct gcccgccgcg ctgctctggg
      481 gcgcgagctt tccgctcgcg ctcgcggccg cgattcgcgc gggcgaagag ccgcgccgcg
      541 cggtcgggcg cctgtatgcc gcgaacacgg ccggcgcgat cgtcggcgcg ctcgcgacga
      601 cgttcgtgct catcgtcgtc gtcggcagtc agcgcgcggc gcagctcatg attctcgcga
      661 gcgtcgcggg cgccgtagtg ctgctcgtcc gctaccacgg ctttacgtcc cgtcggctgc
      721 agcgagccgc cggaatcgcg gcggcgtgcg cggcggccgc cgtcgccgtg cctgcgctgc
      781 ctcccgagct cgtcgcctat ggccgcttcc tgccgacgcg cgggctaaac gcgaacgtcg
      841 tttatgtcgg cgagggcgtc accgcctcga tcgcggtgac gaaggagccg aacggcacgc
      901 tgacgtacca caacgccgga aagacacagg cttcgacgta cccgcaagac atgcgcttgc
      961 agcgcatgct cgggcacctg acgacgctcg tccccgccga gccgcgcaat gttctcgtca
     1021 tcggcctcgg cgccggaatc accgcgggcg ccgtcagcct cgaccccgcg gtcgagcgcg
     1081 tcgtcgtcgc agagctcgag cctctcgtgc cgatcgtcgc cgccggattc ttcgacgtgc
     1141 acaactacta cgtcgtcacg aacgacaagg tcgagatccg catcgacgac gggcggcatg
     1201 cgctcgcgac gacggccgag cgcttcgacg ccatcacgtc cgatccgctc gacccgtggg
     1261 tcaaaggcgc cgccgcgctc tacacgcgcg agttctggca gctgtgcaag gagcggctca
     1321 acgacggcgg cgtcgtgacg gtgttcgtgc cgctctacga gaccaccgaa gacgccgtcc
     1381 gcagcgagct cgagactttc ttcgacgtgt ttccgaacgg cgccgtattc gcgaacaccg
     1441 tcgacggcat gggctacgac gtcgtgctcg tcgggcgcaa cggcgacgag ccgatcgacg
     1501 tcgcagccgt gacgcagcgg atcgagagcg gcgactatgc acgcgtcgcg gcttcgctgc
     1561 gcgccgtcgg tttcgattcg gcgctcgacc tgctgagcac gttcacggcc gacgctgcgt
     1621 cgctcgagcc gtggctcgcc ggcgcaaaga tcacgaccga ccgcaacctg cggctccagt
     1681 acctcgcggg cgaaggcctc aacgtgctcg cggccccgcg gatattcgcg gcgatgacgg
     1741 cctcggctcc gggcgttccg ccgaagctct tcacgggcac gcccgcgcag ctcgaagagc
     1801 tgcggcaacg gctcgcggca cgccagggtc gctactgagc gtaagcgcgg cgcacgggcg
     1861 cgccgtccgg ctatttcatc gtgacgacgt tgactctgcc ttcgcgccgc acgacgttga
     1921 tgcccacgtc ctcgcgatgg cggaccaggt aaagatcgac gctcgcatcg ccgacgcgca
     1981 gcgcgtcgat gtggacctcg cgcaagaact ccggcaacac gggattggcg aacacgactt
     2041 tgcgctcggc gccgcgcact tcgagcccga ggcacgcctg gagcagcatc agcacggagg
     2101 ccgaggccca cgactgcggc gcgcaggaga cgggatattg cgtcgggctt tcgtcgggcc
     2161 tgcgagcgaa accgcagaac agctccggca gccggtgcag atcgaagaac tgactcgcgt
     2221 cgaacaggcc cgtcagcacg cgcagcgcct cggccttgtg cccataccgc gcgaggcccg
     2281 ctgcgatcag cgcattgtcg tgcggccaga tggaaccgtt gtggtaggac atcgggttgt
     2341 aatggggttc cgtctgcgcg agcgtgcgga tgccccaccc cgagtacgag gcgtcgcaga
     2401 gcagcgtcga cgcgagccgt tccgcgcgct cgtcgccgac gatgccggcg tagaggcaat
     2461 gccccgcgtt cgacgcgcgc acgcggcaga gccgcttgcg cccgtcgagc gcgagagcgt
     2521 aggtcgagag gtcgtcgcac cagaagacct cctcgaagcg ctgccgcaat gcctgcgcgg
     2581 catcgtcgag ctcgcgcgcg cgcgcgaagt cgccgagcgc gtgcgcgacc gaagccgcgg
     2641 agcgctttgc cgcgtatacg tagccctgca cctcgcagag cgcgatcggc gcttcggcaa
     2701 aggtaccgtc ccggtggaac accgaatcgc gcgagtcttt ccaaccctgt tgaacgaggc
     2761 cgctctccgt gcggcgcgcg tattcgacga agccgtcggc gtcgagatcg ccgtagcggt
     2821 cgatccagtc gagcgcgtgt tcgacgttcg gccagagagc ctccaacgcg gcgagatccc
     2881 ccgtgcgctc gtaataggcg cccgcgagca tgacgaacag cggcgtcgcg tcgacgctgc
     2941 cgtaatagcg ctcgaacggg acctcgccgg ttgcggccat ttcgcccgag cgcgtttcgt
     3001 gcaagatctt gccgggctgc gcgtcgcgct cgggtcggtc gaactcggct tgcgtcgccg
     3061 cgaggtactt caggaccccc ttcgctacgc cgggattgaa ccacaggcat tcgagcgccg
     3121 tgatgatgcc gtcgcggccg aacggcgtgc tgaaccatgg cacgccggcg tacggatagg
     3181 gaccgtaggg cgtctgcgtg cgcatcatgt gcaggtccgc gagcgagcgc gacaaccagt
     3241 cgttgaacgg ctcgctcgag gtgcgaagct gcggctcgtc ggcgcgcgca cgcgccagta
     3301 tgtcggagac tttgcgggcg gcgtcctcgt acgaggtcgt cggcaacgcg gcacggctgc
     3361 cggcgcgctc gccggacacg acgagctggc acatggcctc accgcgcggc ggcaacttca
     3421 gggcgtactc cgcgcggccg tcgctcaagc gcgtgggcgg cgggtcgaac tcgagcctcg
     3481 tgcggcgcac gcgcccatcg agaccctcgt agccgagctc gagctccgct tcgcggacgc
     3541 gcgccggcaa ctgtctgccg cgccgcgcgc gcagcgtgcc gcgcacctcg aagatgtcgg
     3601 cgaagtcggc gccgcactcg atcgacatcg cgatctcgac gggagagtcg ccgtaattgt
     3661 ggacgcggat acgctcatga caagcgccgt cgagcaggac gcgcgagcgg tcgacgtgga
     3721 ccgtgccgcg cgggatcagc tcgtcgccgc gcttcaagtc cgggttcatg agatcgacgg
     3781 cgagcacggc gttgtcgtcc ttgatcgtcg agctcagcag caagggcctg tgcccgtcca
     3841 agcgcagagc caagcgcgac aagatccgcg tgtcatggtc gtagacgccg agctcgccgg
     3901 cgccgaattg ctcgatgtcg ccgaagcggt cgaatactgc gaacgtgtct ccgtgcttca
     3961 tgactcgggt ccgatcatcc gtgcgggcgg aggtcgagac gatgtagtac tgatccgacg
     4021 ggtgcagagg ttcggcgctc attaggacgt tcccgtcatg cgccgttgcg gcgcttcgag
     4081 caacgccgaa tagagctcga tgtagtcgct cgccatgcga ctggccgcga agcggtcttc
     4141 aaagacttcg cggcaacggc gccggctgat ttcgccgatg cggcgtatgg cggccacggc
     4201 atcgtcgaga tcgtcgacga tgaaacccgt gacgccgtca tcgagaacct cgggcaccga
     4261 gccgccgcgg aacgcgatga cgggcgtgcc gcaagcgagc gcctcgatca tgacgagccc
     4321 gaacggctcg ggccaatcga tgggaaacag caagaggcgc gcgttgccga aaaattcttc
     4381 cttttcgccc tcgccgatct cgccgagata ctcgatcagc ggatcgcgca gcagcggctc
     4441 gacgacgttc tcgaagtact ctcgatctat cgcatcgacc ttcgccgcga tcctgagcgg
     4501 cacgccgacg cgcttcgcga tctcgatcgc gcggtcgacg cgcttctcgg gcgccacgcg
     4561 gccgatgaat gccaggtaat cgccgggctc ggcgtgaaag cgatagaggt cgggcggcag
     4621 gccgtgatgc actgtgccga tccagttcgc gaacggcagc ggctcgcgct gagccccgga
     4681 gatcgagacc acgggcatgt cgcgaaactc gcgatagagc ggctgcagat ccggtatgtc
     4741 gaggcgcccg tgcagcgtcg tcaaatgcgg agtcttgtgc cgccgggagt gcgggaaatg
     4801 caaatagtcg acgtggtaat ggatgacgtc gaacgacgac gcgcgctgca cgacgcgctc
     4861 gagcatcagc acgtgatggg ccatctgatc gacgcagttc ttgtcgagtc gcagcgagcg
     4921 ccgcgacgaa gcgacgagct tcgcctccgt gatggaatcg ccgctcgcga acagcgtgac
     4981 ctcgtggccc tggctaacga gctcctcggt gagataggac acgacgcgct cggtgccgcc
     5041 gtagtacttc ggcggcacgc tctcgaacag cggggcgact tgggcgatac gcataggcat
     5101 ccatgccatg ttctggctcc ctgtatgacg agacagcgcg gccggcgcaa aagttctgcg
     5161 ccgcgcgtct cttatcgcca caaggtagat cagtcgcttt gcgcgagcag gcgagctttc
     5221 gcttttgtca aggcggccag cgttttttcg tcgggctgcg ggaaccgtag atccaacccg
     5281 tcgagcgccg agttgatgag cttcgcgact tgccagcgct ggtaatgttt gtcatccgac
     5341 gggatcgcgt accacggggc ccacggccgc gacgtcacgc gaaagcattc ctcgtacgcg
     5401 gcgacgtagt cgtcccagcg gtcgcgctcg tcgagatcgc cggggttgaa tttccaattc
     5461 ttcttcggct cgtcgatgcg ctcgagaaag cgttcgcgct gctcggcctt ggacacgttg
     5521 agccaaaact tcaagatgat catgccctgc tcggcgaggt agcgctcgtg atcggcgatc
     5581 gctcgcagcc ggttggccca cagcttcggt gaaggctcgt ccggcaggcg ctgcgccgcc
     5641 aggaactccg ggtgcacgcg aacgacgagc acctcttcgt agtagctacg gttgaagatc
     5701 gcgatgtggc cgcgctcggg cagatgcggc gtcgtcctcc agaggaagtc gtgcgcgagc
     5761 tcgagcgagg tcggctgctt gaatgccgcg acgcgcaagc cggccgggtt cacacccgtg
     5821 aaaacgtgac gaatcgtgcc gtccttgccc gcggcgtcga gcgcctgaag gacgatgagc
     5881 acgggatacc tgccgtccgc gtagagccgc tcctgtgcct cgccgagcgt cttggtctcg
     5941 tcctcgagcc gctccttcca gccgttcttg tccttgccct tgggcggcgc cgtcggcgcc
     6001 ttgcgcgcgt cgaacgtacc gtcgaacggc acgagcaagg aatgttcttt ggcggcgaac
     6061 atcgaccacc tctcggaggt gccggtgtta cggttcggtt aaaccgcaac accggcactt
     6121 cggttaaacc gcaacaccgg cacttcggtt caaccgaaac cgtaacaccg gcacctacgg
     6181 tgggggcggc gcggtgcggc actccgtggg gttccacttg ctgccgttcg gctcgcgctt
     6241 gaagtgcgtg ctcgtcagga acgcgtcgtt gagataacgc gggtcc
//
LOCUS       DASSNN010000566         2545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_601778, whole genome shotgun sequence.
ACCESSION   DASSNN010000566 DASSNN010000000
VERSION     DASSNN010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601778"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2545)
                     /locus_tag="VFL84_10495"
     CDS             complement(<1..>2545)
                     /locus_tag="VFL84_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET7609096.1"
                     /translation="ELGIDIGDVDLVCQLGSPHGISAFLQRVGRSGHGLGRIPKGRLF
                     PLSRDDLVECTALLASIKRRELDAICPRPQPLDVLAQQIVAEAGVQEWKVEDLHKRFA
                     HAASYKDLGIDEFVGVARMVAEGFTTHRGRRSAHLHFDRVNNVLRGRRGAALTAVTNG
                     GAIPDQFDYEVVLLPSEVPVGTLNEDFAFESLAGDIFQLGNASYRIQKVESGKVYVTD
                     AAGQPPTIPFWLGEAPGRSRELSSSVGKLNETIGELIDAEGLEGATRWLREQLGLSES
                     AAKQLIDYLGAAKAALGVLPTAKRVVFERFFDETGDTHLVVHSPFGSRVNRAWGLSLR
                     KRFCRRFNFELQAAALEDSIVLSLGAVHSFALEDVVHYLHSNSVRSVLTQAVLDAPLF
                     GVYWRWNASAALAVRRFRNGKKQPAQFQRMDAEDLLSTVFPDQLACAENLPGGDREIP
                     DHPLVKQTLDDCLTGAMDIDGLEEVLRRIEAGEIEVVCRELTSPSPLAQEILGAKPYA
                     FLDDAPAEERRTLAVQARRYMTPEQAAELGQLDPDAIARVRAEAWPQPLNADELHDAL
                     VLLGFVTAQEGAAWISMFERLRADGRATVVELPQAGTVWVAAERLPEVDAAFPGAVAA
                     ERLAVIGGAVEDRDTALRELVRSRFEALGPVTAEALAQPFGLAAAEIMPALLALEQQG
                     TAMRGNFTGAGVTVSVQPNEVPELRLNRNRNTGTEEWCERRLLARIHRYTLKRLRNEI
                     EPATLSDFQRFLFHWQGLGGERRQGREALSAVLGELQGLALPAAVWEREVLPARIADY
                     SALLLDQLSSAGEFVWWRPRPSGAQPGARAATVATSPIALVPRGSLALWR"
BASE COUNT          388 a          918 c          862 g          377 t
ORIGIN      
        1 cgccatagcg caagcgagcc gcgcggcacg agcgcgatcg gcgacgtcgc caccgtcgcg
       61 gcgcgtgcgc cgggctgggc gccgctcggc cgcggccgcc accagacgaa ctcgccggcc
      121 gatgaaagct gatcgagcaa cagcgccgag tagtccgcga tgcgcgccgg cagcacctcg
      181 cgctcccaga ccgccgccgg cagcgccagc ccctgcagct cgccgagcac ggcgctcaaa
      241 gcttcgcggc cttggcgacg ctcgccgccc aacccctgcc agtggaacaa gaagcgctgg
      301 aagtccgaca gcgtggccgg ctcgatctcg ttgcgcagcc gcttcagcgt atagcgatgg
      361 atgcgcgcaa gcagccgccg ctcgcaccac tcttcggtgc cggtgttacg gtttcggttc
      421 aaccgtaact ccggcacttc gttcggttga accgaaaccg taacaccggc acctgtgaag
      481 ttgccgcgca tcgcggtgcc ttgctgctcg agcgcgagca gcgccggcat gatctccgcg
      541 gccgcaaggc cgaacggctg cgccagcgct tcggccgtca cggggccgag cgcctcgaac
      601 cggctgcgca cgagctcgcg cagcgccgtg tcgcgatctt cgacggcgcc gccaatgacg
      661 gcgagccgct cggcggcgac cgcgcccggg aatgcggcgt cgacttccgg caatcgctcg
      721 gcggcgaccc aaaccgttcc ggcctgcggc agctcgacga cggtcgcgcg gccgtcggcg
      781 cgcagccgct cgaacatcga gatccacgcg gcgccttcct gcgccgtcac gaagccgagc
      841 agcacgagcg cgtcgtgcag ctcgtcggcg ttcaaaggct gcggccacgc ttccgcgcgc
      901 acgcgcgcga tcgcgtccgg gtccagctgg ccgagctcgg ctgcctgctc cggcgtcatg
      961 tagcggcgcg cctgcacggc cagcgtgcgc cgctcctcgg ccggcgcgtc gtcgaggaac
     1021 gcgtacggct tcgcgcccaa gatctcctgc gcgagcggcg acgggctcgt cagctcgcgg
     1081 cagacgactt cgatctcgcc ggcctcgatg cgccgcagca cttcctcgag cccgtcgatg
     1141 tccatcgcgc ccgtcaagca gtcgtcgagc gtctgcttga cgagcggatg atcgggaatc
     1201 tcgcggtcgc cgcccggcaa gttctcggcg caggcgagct gatccggaaa caccgtcgac
     1261 aacaaatcct cggcgtccat gcgctggaat tgcgccggct gcttcttgcc gttgcggaaa
     1321 cggcgcacgg cgagcgccgc gcttgcgttc cagcgccagt agacgccgaa cagcggcgcg
     1381 tcgagcacgg cttgcgtcag cacgctgcgc acgctgttgc tgtgcaagta gtgcacgacg
     1441 tcctcgagcg cgaagctgtg cacggcgccg agcgacagca cgatcgaatc ctcgagcgcc
     1501 gcggcttgca gctcgaagtt gaagcggcgg cagaagcgct tgcgcaagct caagccccac
     1561 gcgcggttga cgcgcgagcc gaacggcgaa tgcacgacga gatgcgtgtc gccggtctcg
     1621 tcgaagaagc gctcgaacac gacgcgcttc gccgtcggca gcacaccgag cgcggctttc
     1681 gcggcgccga ggtagtcgat gagctgcttc gcggcgctct cgctcaagcc gagctgctcg
     1741 cgcagccacc gcgtcgcgcc ttcgagccct tccgcgtcga tgagctcgcc gatcgtctcg
     1801 ttgagcttgc cgacggagct cgacagctcg cggctgcggc ccggcgcttc gccgagccag
     1861 aacgggatcg tcggcggctg cccggccgcg tcggtcacat agactttgcc cgactcgact
     1921 ttctggatgc gatagctcgc gttgccgagc tggaagatat cgccggccag gctctcgaac
     1981 gcgaagtcct cgttcaacgt gccgaccggc acttcgctcg gcagcagcac gacctcgtag
     2041 tcgaactgat cggggatcgc gccgccgttc gtgacggccg tcagcgcggc gccgcgccgg
     2101 ccgcgcagca cgttgttgac gcggtcgaag tgcaggtgcg cgctgcgccg cccgcgatgc
     2161 gtcgtgaagc cttccgcgac catgcgcgcg acgccgacga actcgtcgat gccgagatcc
     2221 ttgtagctcg ccgcgtgcgc gaagcgcttg tgcaggtcct cgactttcca ttcctgcacg
     2281 ccggcctcgg cgacgatctg ctgcgcgagc acgtcgagcg gctgcggtct cgggcagatc
     2341 gcgtcgagct cgcggcgctt gatgctcgcg agcagcgccg tgcactcgac gaggtcgtcg
     2401 cgcgacagcg ggaacaagcg gcccttcggg atgcggccga ggccgtggcc cgagcggccg
     2461 acgcgctgca agaacgccga aatgccgtgc ggcgagccga gctggcagac caagtcgacg
     2521 tcgccgatgt cgatgcccaa ttcga
//
LOCUS       DASSNN010000567         4144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_686050, whole genome shotgun sequence.
ACCESSION   DASSNN010000567 DASSNN010000000
VERSION     DASSNN010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686050"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..723)
                     /locus_tag="VFL84_10500"
     CDS             complement(235..723)
                     /locus_tag="VFL84_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HET7609097.1"
                     /translation="MESTTVVLWIASGVLIAIGAIGLVLPILPGAPLIFIGALLAAWA
                     EDFMYIGPGSLIVLGILTALAVAVDFVAGAFGARRFGASARAVTGATLGTIVGLFFGV
                     VGVIVGPFVGAVIGELSVRRNLAAAGRAGIGATLGLALGTAAKLAIAFAMIGVILFMR
                     LL"
     gene            complement(730..858)
                     /locus_tag="VFL84_10505"
                     /pseudo
     CDS             complement(730..858)
                     /locus_tag="VFL84_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(895..1560)
                     /locus_tag="VFL84_10510"
     CDS             complement(895..1560)
                     /locus_tag="VFL84_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609098.1"
                     /translation="MRRAFKLSLGLVVLFAALSIVLLAATIYTYDRLSGETLIAELRF
                     DATGDRQYLAHLRTGDRCEDRTFPLYGDQWRIDAEFLKWKYWALLLGLDSQYRLDRLE
                     GRYRSVAEQNSQRNVAHALGERTAVDVVALAESLGSWNFLLDATYGSSTYQDIDVGDV
                     YYVYRTTTGIITRHEPRPVQTGGGAAFDVTIDGACNDPSMWQRFTEWTDDGVVAALEL
                     VER"
     gene            1653..2276
                     /locus_tag="VFL84_10515"
     CDS             1653..2276
                     /locus_tag="VFL84_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7609099.1"
                     /translation="MLTIYGRTNSVNVQKVLWCLAELAVPYTRIDAGLEHGKNTEAWY
                     LKLNPNGKVPLLIDGSFTLWESNTIVRYLAGKHGMGSLCPAAFEARALAEAWMDWQLS
                     TLVGPVSVVFRNLVRKPIGERDAAAIERNLREANRAMTLLDDHLKRVPYVAGEAFTMG
                     DIPVGATAHRWLEIEGIDRPPLTAVRAWHDRLAQRAGFREHVQLPLS"
     gene            2463..>4144
                     /locus_tag="VFL84_10520"
     CDS             2463..>4144
                     /locus_tag="VFL84_10520"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7609100.1"
                     /translation="MRIAYRSLAVAYALAFFTLHASAENELFPRPAELEPAVQFWTRV
                     YTEIDTSEGFIHDDLRLNIVYQTVHTGDLSSRERRRRVERAQETTRNILTKLAGGART
                     GLNGEEERILKLFPEGTSNAEFRAAAGRVRFQLGQSDRFREGLVRSGTFKPYILGVLA
                     KRGLPLELAALPHVESSFDPTAYSKVGAAGLWQFTRSTGVRYMRIDHVIDERRDPYFA
                     TDAAARLLADNFSVIQSWPLALTAYNHGLAGMRRAVEVHKTSDISTIVAKYQGRTFGF
                     ASRNFYAAFLAALDIDSNPERYFPNLKLDAPSDTETIPVPAFMTVDTLADALNVREST
                     LRNLNPALTEIVWSGDKYVPQGFELRVPRETAAVAEALLGTIDANKMFAAQRPDREHK
                     VRRGDTLSRIAAEYRVSLAALMRVNGMTNKSVLRVGQSIRLPTDGVTAPPDATLVRAE
                     APAERAPAVVTPTLAATSAEGIYVVRRGDSIERIATRLGVDPQALIAANDIRNRNVIQ
                     IGQQLIIPTAPGAVVALAAVATQPAPTTDAPVVAAGESPAAVAAAPAASADVT"
BASE COUNT          752 a         1421 c         1322 g          649 t
ORIGIN      
        1 cgtcggtaaa gacgttcagc agcgggaacg cacccgaacc ggtcgtgcgg ctgccgaacc
       61 agtcgctgcc catcacactc tccatcgcac gctgcacacc gagcaacgtc gcgaacggat
      121 ctctgactcg aatgaccata agtacccctc ctcgcatggg gcgaaatttc cgttggccct
      181 tgtgtggggc cggcactttg gcgattcaag gccggctttc ggaatcgaag agagctacag
      241 gaggcgcatg aaaaggatga cgccgatcat cgcaaaggcg atcgcgagct tggcggccgt
      301 gccgagcgca agaccgagcg tggcgccgat gccggcgcgt ccagcggccg caagattgcg
      361 ccgcaccgaa agctcgccga tcacggcgcc gacgaacggc ccgacgatca cgccgacgac
      421 gccgaagaac agccccacga tcgttccgag cgtcgcgccc gtgacggcgc gcgccgacgc
      481 gccgaaccgc cgtgccccga atgcgccggc gacgaagtcc acggcgacgg ccagtgcggt
      541 caagatgccg agcacaatca gcgaccccgg gccgatatac atgaaatcct cggcccatgc
      601 ggccagcaag gccccgatga agatcaacgg agcgcccggc aatatcggca acacgagccc
      661 gatcgcgccg atcgcgatca gcacgccgga agcgatccaa agtacgacgg tggtcgactc
      721 cacgctcgct tagttcaaat cgggcgtgcg cagcttcttg ctatgccacg ccttgttgcg
      781 cacgtgcgtg cgcgatttga tgcgccagcc gtccggcgtg cgcacgtaaa cgtcctcgta
      841 acggcgagcg agcgcatgga gctgttcatc ggtgttcttc ctcgcagggc ttacttatcg
      901 ttcgacgagc tcgagcgcgg cgacgacgcc gtcgtcggtc cactcggtga agcgctgcca
      961 catcgacggg tcgttgcacg cgccgtcgat cgtgacgtcg aatgccgcgc cgcctccggt
     1021 ctgaacgggc cgcggctcgt ggcgcgtgat gatgcccgtc gtcgtgcggt agacgtaata
     1081 gacgtcgccg acgtcgatgt cctgatacgt ggagctgccg taggtcgcgt cgagcaagaa
     1141 attccacgag ccgagcgact cggcgagcgc cacgacgtcg acggccgtgc gctcgccgag
     1201 cgcgtgagcg acgttgcgct gcgaattctg ctcggccacc gagcggtacc ggccttcgag
     1261 ccggtcgagc cgatactgcg agtcgagccc gaggagcagc gcccagtatt tccacttcag
     1321 gaactcggcg tcgatgcgcc attggtcgcc gtacagcggg aacgtccggt cctcgcaccg
     1381 atcgccggtg cgcaagtgcg cgaggtattg acgatcgccg gtcgcgtcga acctgagctc
     1441 cgcgatcagc gtctcacctg aaagcctgtc gtacgtatag atcgtcgccg ccaagagcac
     1501 gatcgataac gccgcgaaca gcacgacgag cccaagcgat aatttgaacg ctctcctcat
     1561 gaagcgcctt ccctaggagt cggcacgccc gccgatgccg tgcgagaata ccacgccatc
     1621 cgtcaccgtc ccggcaagcc gaggagcgcc gcgtgctgac catctacggc cgcacgaact
     1681 ccgtcaacgt gcagaaggtg ctctggtgtc tcgcagagct tgcggtgccg tacacgcgga
     1741 tcgacgccgg cctcgagcac ggcaagaaca ccgaggcgtg gtacttgaag ctgaatccca
     1801 acggcaaggt gccgctgctg atcgacggct cgttcacgct gtgggagtcg aacacgatcg
     1861 tgcgctactt ggcgggcaag cacggcatgg gctcgctttg cccggccgcg ttcgaagcgc
     1921 gcgcgctcgc cgaagcgtgg atggactggc agctctcgac gctggtgggc ccggtgtcgg
     1981 tcgtgttccg gaacctcgtc cgcaagccga tcggcgagcg ggacgcggcc gcgatcgagc
     2041 gcaaccttcg tgaggcgaat cgcgcgatga cgctgctcga cgatcacttg aagcgagtgc
     2101 cttatgtcgc aggcgaggcc ttcacgatgg gcgacatccc cgtcggagcg acggcgcacc
     2161 gctggctcga gatcgaaggg atcgaccggc cgccgctaac ggcggtgcgg gcttggcacg
     2221 accgcctcgc gcagcgcgcg gggttccgcg agcacgtcca gctacccttg agctaaaggc
     2281 tttgacggca cgcaggaggt gcggtaattt ccggtcgtaa gccgatagaa acaatgacaa
     2341 aaaccggtta cgggagaccg ccttctcggc ccttcggacg tgaactgcgt ccgggacggc
     2401 cggcgggcgc aatggacata atccaagaac gacttgggct cgcaggattc gggaagcact
     2461 acatgcgcat tgcatatcgc tcgctggcgg tagcctacgc cctcgccttt ttcacacttc
     2521 acgcgagtgc agagaacgag ctctttccgc ggcccgcgga gctcgagccc gccgtccaat
     2581 tctggactcg cgtctacacc gagatcgaca cgagcgaagg cttcatccat gacgacctgc
     2641 gcttgaacat cgtctatcag acggtgcaca cgggcgactt gagctcgcgc gaacggcgcc
     2701 gccgcgtcga gcgcgcgcag gaaacgaccc gcaacattct cacgaagctc gccggcggcg
     2761 cgcgcacggg gttgaacggc gaggaagagc gcattctcaa gctgttcccc gaaggcacga
     2821 gcaacgccga gtttcgcgcc gcagcggggc gcgtgcgctt ccagctcgga caatcggatc
     2881 ggttccgcga gggcttggtc cgctccggca cattcaagcc ctacattctc ggggtgctcg
     2941 cgaagcgcgg cttgccgctc gagctcgcgg cgctgccgca cgtcgagtcg tcgttcgatc
     3001 caaccgctta ctcgaaagtc ggcgcggccg gcctctggca attcacgcgc tcgacgggcg
     3061 tgcgctacat gcgcatcgat cacgtcatcg acgagcgccg cgatccgtac ttcgccaccg
     3121 acgccgcggc gcggcttctc gccgacaact tcagcgtcat tcagtcctgg ccgctagcgt
     3181 tgacggccta caaccacggc ctcgccggca tgcgccgcgc ggtcgaagtg cacaagacgt
     3241 ccgacatctc gacgatcgtc gcgaaatatc agggccgtac gttcggcttc gcgtcgcgca
     3301 acttctatgc ggcgtttctt gccgcgctcg acatcgactc gaatcccgag cgctacttcc
     3361 cgaatttgaa gctcgacgcg ccgtcggata ccgaaacgat cccggtgccg gcgttcatga
     3421 ccgtcgacac gctcgccgac gcgttgaacg tccgcgaaag cacgctgcgc aacttgaacc
     3481 ccgcgctcac cgagatcgtg tggtcgggcg acaagtacgt gccgcaaggc ttcgagctac
     3541 gcgtgccgcg cgagacggcc gccgtcgccg aggcgctgct cggcacgatc gacgcgaaca
     3601 agatgttcgc ggcgcagcgc ccggaccgcg agcacaaggt gcgacgcggc gacacgctgt
     3661 cgaggattgc cgccgagtac cgcgtgagcc tcgcggcgct gatgcgcgtc aacggaatga
     3721 cgaacaaatc cgtgctgcgc gtcggtcagt cgatccgctt gcccacggac ggcgtcacgg
     3781 ccccgcccga tgcgacgctc gtgcgcgccg aggctccggc cgagcgcgca cccgccgtcg
     3841 tcactccgac cttggccgcg acgagcgccg aaggcattta cgtcgtgcgt cgcggcgaca
     3901 gcatcgagcg catcgcgacg cgcctcggcg tcgacccgca ggccctgatc gcagccaacg
     3961 acattcgcaa ccgcaacgtc atccagatcg gccaacagct gatcattccg acggctccgg
     4021 gcgccgtcgt cgcgctcgcc gccgtagcga cgcagcccgc gccgaccacg gacgcgcccg
     4081 tcgtcgcagc cggcgagtcg cccgccgccg tcgccgccgc gccggccgcc tcggccgacg
     4141 tgac
//
LOCUS       DASSNN010000568         4644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1700102, whole genome shotgun sequence.
ACCESSION   DASSNN010000568 DASSNN010000000
VERSION     DASSNN010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700102"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFL84_10525"
     CDS             complement(<1..193)
                     /locus_tag="VFL84_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609101.1"
                     /translation="MTKTRIVFAVSVLLALAGAAGAQPPGITAEMINTTLPLEGAPLA
                     VPGPYQTMSEPTFGKPGLVL"
     gene            298..1521
                     /locus_tag="VFL84_10530"
     CDS             298..1521
                     /locus_tag="VFL84_10530"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003270147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HET7609102.1"
                     /translation="MSGEQYQTMDVEGGRQVKLWTRGVPVDPKAKAQLANTAKMPFIF
                     KHLAAMPDVHVGIGATVGSVVATKGAIIPAAVGVDIGCGMIATQTTLKAADLPESLGK
                     IRSSIERAVPHGFVSIPGRSVKGAWSTTPDSVITRWRGLAERLDRIEDKYPRLKAKTP
                     QYQLGTLGGGNHFIEICLDTEQTVWVMLHSGSRGIGNLIGQTFIELARSDMKKHFINL
                     PDRDLAYFAEGTQHFDDYVEAMQWAQDYAAENRRAMMDAVLRVLREELRPFKLGDVAV
                     NCHHNYTARETHFGEDVLVTRKGAVRAGRGELGIIPGSMGARSYVVRGLGNPESFSSC
                     SHGAGRTMSRTEARKRFTVDEHVAATKGVECRKDADVIDETPAAYKNIDAVMEAQCDL
                     VEVVHTLKQVVCVKG"
     gene            1597..2244
                     /locus_tag="VFL84_10535"
     CDS             1597..2244
                     /locus_tag="VFL84_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609103.1"
                     /translation="MNVRSVVLLLAAAPLGAQAQSQAPLPPDIHPVTLSRLPPVTPED
                     LDEEARRLFNERQNFTPGPGPTHVTVYIPRERSLGVPTGENSPVGPRFFQLAVLIAAR
                     EIDQQYEWSAHEPAGLRQGLEQSVIDVVKYDRPVTGLAEKDATLITFGRTLLRDNKVS
                     SEVWADMVRLFGRQHTIDLLGIMGDYLRVGIMLNAVDQRQPPTRPALLPPLDRKR"
     gene            2290..3264
                     /locus_tag="VFL84_10540"
     CDS             2290..3264
                     /locus_tag="VFL84_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET7609104.1"
                     /translation="MKAAVYREYGPPEVVRIEEVPKPKPSAGEVVVRVVASTLCTADW
                     RLRRPSPPPMGWAMNGFRRPKKIHVLGMEFAGTVDAVGNGVRNFKGGERVFGASWRFG
                     AHAEYACFAERSLALIPDGVGFAEAAAIPYGGLTALQFLRLARIKAGQNVLVRGASGS
                     VGSASVQIAKHFGAQVTGMCSSANVELVRSIGADAVVDYTHEDFARAGRVYDIVHDTV
                     GGFGYRRARRVLKRGGVFIDAGPGLASLLVGPLLRLTGRGNVVGSVAKGGREQLEFLA
                     SLVAQRRFKPVIERRYRLSEIVAAHRHAETGHKKGNLIIDLALVPAGR"
     gene            3360..4220
                     /locus_tag="VFL84_10545"
     CDS             3360..4220
                     /locus_tag="VFL84_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7609105.1"
                     /translation="MRHLKTVSVAAVAAGVVLYLAGVTALWAFQRDLMYFPDGLPRVP
                     PSYYEMLDGVQEVSFTTADGIDLVAWYAPAPPNRPTVALFHGNGGSLRGERYRLKHFK
                     DAGLGVLLLAYRGYSGNGGSPSEQGLYADARAALDWLQASGVDGRAIVLYGISLGSGV
                     ATKMAAEREVGAVVLESPYTSTVDVAAWRFPIVPVSWLMEDRFESLARIGAVTEPVLV
                     MHGDADFVVPQRFGKQLFDAANEPKAGFWPHGVGHNDVFDNGGFDRALEFIRSTLHVP
                     IDTQSVANGA"
     gene            complement(4225..>4644)
                     /locus_tag="VFL84_10550"
     CDS             complement(4225..>4644)
                     /locus_tag="VFL84_10550"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7609106.1"
                     /translation="QQIDGTMASERVLATLSSWFGVLALALACVGLYGVVAHDVARRR
                     REIGIRLALGARPAAVEHETLKRAGLLVVAGVTGGLAAAFAATRFLGGLLYGLGPTDP
                     PSIAAGVAVLALTALAASYLPARRAARIRPAEVLRSE"
BASE COUNT          778 a         1524 c         1612 g          730 t
ORIGIN      
        1 agagcacgag gcccggcttg ccgaacgtcg gctcggacat cgtttgatac gggcccggga
       61 ctgcgagcgg cgcgccttcg agcggcagcg tcgtgttgat catttccgcc gtgatgccgg
      121 gcggttgtgc gccggctgcg ccggccaaag cgagcaacac cgaaactgcg aaaacgatgc
      181 gtgtttttgt catgaccacg tctcccccgg ggcgtttgtc ctccgcacca tgctacggtt
      241 tttcgcgcta tggtgcgaat ggggcaccgg gtgcgcacgg acccagtcga agacgacatg
      301 agcggcgagc aataccaaac catggacgtc gagggcggcc gccaggtcaa gctgtggacg
      361 cgcggcgtgc ccgtcgaccc gaaagcgaag gcgcagctcg cgaacacggc gaagatgccg
      421 ttcatattca agcacctcgc cgcgatgccg gacgtgcacg tcgggatcgg cgcgacggtc
      481 ggctccgtcg tcgcgacgaa gggcgcgatc ataccggccg ccgtcggcgt cgacatcggc
      541 tgcggcatga tcgcgacgca gacgacgctg aaggcggccg acttgcccga gtcgctcggc
      601 aagatccgct cgagcatcga gcgcgccgtg ccgcacgggt tcgtctcgat tccggggcgc
      661 agcgtgaagg gcgcctggtc gacgacaccg gactcggtca tcacgcgctg gcgcggtctc
      721 gccgaacggc tcgaccgcat cgaggacaag tacccgcgcc tgaaggcgaa gacgccgcag
      781 taccagctcg ggacgcttgg cggcggcaat catttcatcg agatctgcct cgacaccgag
      841 caaaccgtct gggtcatgct gcattcgggc tcgcgcggca tcggcaactt gatcgggcag
      901 acgttcatcg agctcgcgcg ctcggacatg aagaagcact tcatcaatct gccggaccgc
      961 gatctcgcgt atttcgccga gggcacgcag catttcgacg actacgtcga agcgatgcag
     1021 tgggcgcagg actacgcggc ggagaaccgc cgcgcgatga tggacgccgt tttgcgcgtg
     1081 ctgcgcgagg agctgcgtcc gttcaagctc ggcgacgttg ccgtgaactg ccaccacaac
     1141 tacacggcgc gcgagacgca cttcggcgag gacgtgctcg tgacgcgcaa gggcgccgtg
     1201 cgcgcaggcc gcggcgagct cggcatcatc ccgggcagca tgggcgcgcg ctcctacgtc
     1261 gtccgcggcc tcggcaatcc cgagagcttc tcaagctgca gccacggcgc gggccgcacg
     1321 atgtcgcgca ccgaggcgcg caagcgcttc accgtcgacg agcacgtcgc ggcgaccaag
     1381 ggcgtcgagt gccgcaagga cgccgacgtc atcgacgaga cgccggcggc gtacaagaac
     1441 atcgacgccg tcatggaggc gcagtgcgat ctcgtcgagg tcgttcacac gttgaagcag
     1501 gtcgtctgcg tcaagggatg aattgccgtc gaaccggtga cagcctccgt tctcgcggta
     1561 tagagtcgag ccacgctcga gaaagggggg tggagcatga acgttcgatc cgtggtcctg
     1621 ttgctcgcgg ccgcgccgct cggcgcgcaa gcgcagagcc aagcgccgct gccgcccgac
     1681 atccaccccg tcacgctatc tcgcttgccg cccgtgacgc ccgaggatct cgacgaggag
     1741 gcgcggcggc tcttcaacga acggcagaat ttcacgccgg gccccggccc gacgcacgtc
     1801 accgtctaca tcccgcgcga gcgcagcctc ggcgttccga ccggcgagaa cagtccggtc
     1861 ggcccgcgct tcttccagct cgccgtgctg atcgcggcgc gcgagatcga ccagcaatac
     1921 gagtggtcgg cgcacgagcc ggcgggcttg cggcaaggcc tcgagcagtc cgtcatcgac
     1981 gtcgtgaaat acgaccggcc cgtgacgggg ctcgccgaga aagacgccac gctgatcacg
     2041 ttcggccgca cgctgctgcg cgacaacaag gtcagcagcg aggtctgggc cgatatggtg
     2101 cgcctcttcg gccggcagca tacgatcgat ctgctcggga tcatgggcga ctatctgcgc
     2161 gtcggcatca tgttgaatgc cgtcgatcag cggcagccgc cgacgcggcc ggcgctcttg
     2221 ccgccgcttg atcgcaagcg ctaaccgcgc cgtgatcgac ggtagcacgc cgatgcgatt
     2281 gcccactcga tgaaggccgc ggtctaccgg gaatacggtc cgcccgaggt cgtgcgcatc
     2341 gaggaagtcc cgaagccgaa acccagcgcc ggcgaggtcg tcgtgcgcgt cgtcgcgtcg
     2401 acgttgtgca cggcggattg gcggctcagg cggccgagcc cgccgccgat ggggtgggcc
     2461 atgaacggct ttcggcggcc gaagaagatc cacgtgctcg gcatggagtt cgccggcacg
     2521 gtcgacgcgg tcgggaacgg cgtccgtaac ttcaaaggcg gcgagcgagt gttcggcgcg
     2581 agctggcgct tcggcgcaca cgcggaatac gcgtgcttcg ccgagcgctc gctggcgctg
     2641 attccggacg gagtcggttt cgcggaagcc gcggcgattc cctatggcgg gctaacggcg
     2701 ttgcagttcc tgcggctggc ccgtatcaag gccggacaga atgtcttggt tcgaggtgcg
     2761 tccggctccg tgggcagcgc gtcggtccag atcgcgaaac acttcggcgc gcaggtcacc
     2821 ggcatgtgca gctccgcgaa cgtcgagctc gtgcgctcga tcggtgctga cgccgtcgtc
     2881 gactacacgc acgaagattt cgcgagggca gggcgcgtct acgacatcgt tcacgacacc
     2941 gtcggcggat tcggctatcg cagggcgcgg cgcgtgctga agcgcggcgg cgtcttcatc
     3001 gacgcgggac cggggcttgc gtcgctgctc gtggggccgt tgcttcggct gaccggccgc
     3061 ggcaacgtcg tcgggagcgt ggcgaagggc ggccgcgagc agctcgagtt tctcgcgtcg
     3121 ctcgtcgcgc agcgcaggtt caagccggtc atcgagcggc gttaccgcct gagcgagatc
     3181 gtcgcggcgc accggcacgc cgagacaggc cacaagaagg gcaatctgat catcgacctc
     3241 gcgctggtac cggccggccg ctgacgagcg gcacatttcg tcagaattcg accgtaacgc
     3301 tgccaagttt ttcgcggaga atgtggccgt agcgaaggga ttgtgcgaac ggaaagcgca
     3361 tgcgacatct gaaaaccgtt tcggtcgctg ccgtcgccgc gggtgtcgtt ctctacctcg
     3421 ctggtgtcac ggcgctgtgg gcgttccagc gcgacctcat gtactttccc gacggcttgc
     3481 cgcgcgtgcc gccgtcgtac tacgagatgc tcgacggcgt gcaagaagta tcgttcacga
     3541 cggccgacgg catcgatctc gtcgcgtggt acgcgccggc gccgccgaat cggccgaccg
     3601 ttgcgctgtt tcacggcaac ggcggatcgt tgcgcggcga gcgctatcgc ttgaagcact
     3661 tcaaggacgc tgggctcggc gtgctgctgc tcgcgtaccg cggttattcc ggcaacggcg
     3721 gctcgccgag cgagcaaggc ctctacgccg atgcgcgcgc ggcgctcgac tggctgcagg
     3781 cgagcggcgt cgacggccga gcgatcgtgc tgtacggaat ttcgctcggc agcggcgtcg
     3841 cgacgaagat ggcggccgag cgcgaggtcg gcgccgtcgt gctcgaatcg ccctacacgt
     3901 cgacggtcga cgtcgcggca tggcgttttc cgatcgtgcc cgtgagctgg ctcatggagg
     3961 accgattcga gtcgttggcg cgcatcggtg cggtcacgga gccggtgctc gtcatgcacg
     4021 gcgacgccga cttcgtcgtg ccgcagcgtt tcggcaagca attattcgac gccgcgaacg
     4081 agccgaaggc aggcttctgg ccgcacggcg tcggtcacaa cgacgttttc gacaacggcg
     4141 gcttcgacag ggcgctcgag ttcatccgga gtacgctgca cgtgccgatc gatacgcaaa
     4201 gcgtcgcgaa cggcgcttga agcgtcactc ggagcgcaac acctcggccg gacggatccg
     4261 cgccgcacgc cgcgcgggga gataagaagc cgcgagcgcc gtcagcgcaa gcacggcgac
     4321 tccggctgcg atcgacggcg ggtcggtcgg accgaggccg tagagaaggc cgccgagaaa
     4381 ccgcgtcgcg gcgaacgcgg ctgcgaggcc gccggtgacg ccggccacga cgagcaagcc
     4441 ggctcgcttc agcgtctcgt gctcgaccgc agcgggtcgg gcgccgagcg caagtcgaat
     4501 gccgatctcg cgccggcgcc gcgccacgtc atgcgcaacg acgccataga gcccgacgca
     4561 ggcgagtgcg agcgccagca ctccgaacca cgacgacaac gtcgcgagca ctcgctcgct
     4621 cgccatcgtg ccgtcgatct gctg
//
LOCUS       DASSNN010000569         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_97988, whole genome shotgun sequence.
ACCESSION   DASSNN010000569 DASSNN010000000
VERSION     DASSNN010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97988"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..546)
                     /locus_tag="VFL84_10555"
     CDS             complement(46..546)
                     /locus_tag="VFL84_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HET7609107.1"
                     /translation="MATRQGRRLANAAGVGIVIALMGYALYEEHVVGLEACPLCILQR
                     MAMIGLGFVFAVAALHAPRGAGARVYAVLGALVALVGMGISGWHVRLQNLPPAEVPAC
                     GPGYDYIMSTFGWLDGLSMIFTASGECAEVNWTFLGLSMPAWVFVWFVALGALAVVAN
                     WSRLSR"
     gene            complement(546..1286)
                     /gene="phoU"
                     /locus_tag="VFL84_10560"
     CDS             complement(546..1286)
                     /gene="phoU"
                     /locus_tag="VFL84_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET7609108.1"
                     /translation="MEVEDLGHHISRRFNEDLERVRNSVLSMGGLVEEQLDRAVKALS
                     EGDSRLGLQVAEDDVKVNRMEVSIDEECSRILATRAPTAGDLRLVIAIIKTITDLERM
                     GDEAEKIGVLAARLASVERPSTNYRELRNLARHVKQMVHGALNAFARLDTKGALEIVK
                     SDSAVDEEYESIYRQGITFMMEDRSTISRVMDVTWMARALERIGDHAKNICEYVIFMV
                     HGKDVRHVRLESIEAELEQRARRLESRT"
     gene            complement(1314..2624)
                     /gene="phoR"
                     /locus_tag="VFL84_10565"
     CDS             complement(1314..2624)
                     /gene="phoR"
                     /locus_tag="VFL84_10565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HET7609109.1"
                     /translation="MTLPSRWASQLAALAALLAAALIFGLWFGGAGWWLAGAALLYVA
                     FTLRHLYWLDRALEGHRRVPLFETRGLWAETFARVEKLRTKARNRKKKYHRLLREVRE
                     STGALADGGIILNAEHEIMWFNPAAARLLGLEHATDIGHRIDNLLRHPDFVAYLKAPT
                     GDGLTVASPRHDTGWLHVQIIPYGTDQRLAIVRDVTNEKNVERTRRDFVANASHELRS
                     PLTVISGYLEALRETGDVPDQWQTPIAEMQRQADRMTRILRGLIELTRLESAEASADR
                     DFVDVTSMLKAIVQELQGRAGPSVELVLETDAALLGEESELHSIFYNIVNNAVRFTPP
                     SGSVKVVWRVADGAAVFEVVDTGIGIPEEQIPRVTERFYRVDPGRSRASGGTGLGLAI
                     VKHALQRHEGELVIASEEGKGSTVSCRFPRDRVVFRSDAVSAVL"
     gene            complement(2621..>2829)
                     /locus_tag="VFL84_10570"
     CDS             complement(2621..>2829)
                     /locus_tag="VFL84_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7609110.1"
                     /translation="LEFFMRHPDRVYSRAQLLDRVWGGNVYVEERTVDVHIRRLRKAL
                     TPTAHDGFIRTVRGAGYRFSVGRS"
BASE COUNT          468 a          974 c          928 g          459 t
ORIGIN      
        1 gcagccccgc cgccggagcg atgcgcgata acgaagcttt ccgctctatc tggacaggcg
       61 cgaccagtta gcgacgacgg cgagcgcgcc gagcgcgacg aaccacacga acacccatgc
      121 cggcatgctg agcccgagaa aggtccagtt gacctcggcg cactcgcccg atgccgtgaa
      181 gatcatcgac aggccgtcga gccagccgaa cgtcgacatg atgtagtcgt agccgggccc
      241 gcacgccggc acctcggccg gcggcagatt ttgcagccgc acgtgccagc ccgagattcc
      301 catgccgacc agcgccacga gcgcgccaag gaccgcgtac acgcgcgcgc cggcgccgcg
      361 cggcgcgtgc agcgccgcga ccgcgaacac gaagcccagc ccgatcatcg ccatgcgttg
      421 caggatgcac agcgggcacg cctcgaggcc tacgacgtgc tcctcgtaga gcgcgtagcc
      481 catcagagca atgacgatgc cgacgccggc cgcgttcgcg aggcgccgtc cctggcgtgt
      541 ggcgatcacg tgcggctctc caagcggcga gcgcgctgct cgagctcggc ctcgatgctt
      601 tccaaacgga cgtgccgaac gtccttgccg tgcaccatga agatcacgta ctcgcagatg
      661 ttcttcgcgt ggtcgccgat gcgctcgagc gcgcgcgcca tccacgttac gtccatgacg
      721 cggctgatcg tgctccggtc ttccatcatg aacgtgatgc cttggcgata gatcgactcg
      781 tactcctcgt cgacggcact gtcggacttc acgatctcga gcgcgccttt cgtgtcgagc
      841 cgcgcgaaag cgttgagcgc gccgtgcacc atttgcttga cgtgccgcgc gaggttgcgc
      901 agctcgcgat agttcgtcga cggccgctcg acgctcgcga ggcgcgccgc caggacgccg
      961 atcttctcgg cctcgtcgcc catgcgctcg aggtcggtga tcgtcttgat gatcgcgatg
     1021 acgagccgca agtcgccggc cgtcggcgcg cgcgtcgcga ggatgcggct gcactcctcg
     1081 tcgatgctga cctccatgcg attgaccttg acgtcgtcct cggcgacctg aaggccgagc
     1141 cggctgtcgc cctcggacaa cgctttgacg gcgcgatcga gctgctcctc gacgagcccg
     1201 cccatcgaga ggacggagtt gcgcacgcgc tcgagatctt cgttgaagcg ccgcgagatg
     1261 tggtgaccga gatcttcgac ttccatggcc gcgaatacct tgccgccggc gaactatagt
     1321 acggccgaga cggcgtcgct acgaaacacg acgcggtcgc gcggaaatcg gcagctgacg
     1381 gtgctgcctt tgccctcctc gctcgcgatc acgagctcgc cttcgtgcct ttgcagcgcg
     1441 tgcttgacga tcgcgagccc caagcccgtg ccgccgctcg cgcgcgagcg gcccgggtcg
     1501 acgcgataga agcgttccgt gacgcgcgga atctgctcct cgggtatccc gatgccggtg
     1561 tcgacgacct cgaacacggc ggcgccgtcg gccacgcgcc agacgacttt gacgctgccg
     1621 ctcgggggcg tgaaccgcac ggcgttgttg acgatgttgt agaagatcga atggagctcg
     1681 gactcttcgc cgagcaacgc ggcatccgtc tcgaggacga gctcgacgct cggtccggcg
     1741 cgcccctgga gctcctgcac gatcgccttc aacatgctcg tgacgtcgac gaagtcgcgg
     1801 tccgcgcttg cttccgccga ctcgaggcgc gtgagctcga tcaagccgcg gaggattcgc
     1861 gtcattcgat cggcttggcg ctgcatctcc gcgatcggcg tctgccactg atcgggcacg
     1921 tcgccggtct cgcgcaacgc ctcgagataa ccgctgatga ccgtcaacgg cgagcgcagc
     1981 tcgtgcgagg cattcgcgac gaaatcgcgc cgcgtgcgtt cgacgttctt ctcgttcgtc
     2041 acgtcgcgaa cgatcgcgag cctctgatcg gtcccgtacg gaatgatctg cacgtgcagc
     2101 catcccgtgt cgtggcgcgg cgacgcgacg gtgaggccgt cgccggtggg cgccttgaga
     2161 taggcgacga agtccgggtg acgcagcaga ttgtcgatgc ggtggccgat gtccgtcgca
     2221 tgctcgagcc cgagcaagcg cgcggccgcc gggttgaacc acatgatctc gtgctcggcg
     2281 ttcaagatga tgccgccgtc ggcgagcgcg cccgtgctct cgcggacctc gcgcagcaac
     2341 cggtgatact tcttcttgcg attgcgcgcc ttcgtccgca atttctcgac gcgcgcgaag
     2401 gtttcggccc acaggccgcg cgtctcgaac agcggcacgc gccgatgacc ttcgagcgcg
     2461 cgatcgagcc agtagaggtg acgcagcgtg aacgccacgt acagcagcgc ggcaccggcg
     2521 agccaccaac cggccccgcc gaaccaaaga ccgaagatca gggccgcggc gagcagcgcg
     2581 gcgagcgcgg cgagctgcga ggcccagcgg ctcggcaagg tcacgaccgg ccgactgaga
     2641 accgatagcc cgcgccgcgc acggtcctga tgaacccgtc gtgcgcggtc ggcgtcaacg
     2701 ccttgcgcag ccggcgaatg tggacgtcga cggttcgctc ctcgacatag acgttgccgc
     2761 cccagacgcg gtcgagcagc tgcgcgcggc tatagacgcg gtcgggatgg cgcatgaaga
     2821 actcgagca
//
LOCUS       DASSNN010000570         2579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_961043, whole genome shotgun sequence.
ACCESSION   DASSNN010000570 DASSNN010000000
VERSION     DASSNN010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961043"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFL84_10575"
     CDS             <1..580
                     /locus_tag="VFL84_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HET7609111.1"
                     /translation="AEHEFNASTFPARVIAGTGSDMHACITGAIGALRGPKHGGANEV
                     ALEIQERYATPEEAVADIRARLARKELVIGFGHPVYTVADPRNRVIKDVARRLSEEAG
                     DLTLFRVADAIEAEMAAAKKMFANLDWFSAVAYHVMEVPTPMFTPLFVIARTTGWAAH
                     VIEQRADGKIIRPSANYVGPENRRFVPLAERG"
     gene            complement(581..1084)
                     /locus_tag="VFL84_10580"
     CDS             complement(581..1084)
                     /locus_tag="VFL84_10580"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET7609112.1"
                     /translation="MNASSGKTAYDFTAMDIDGRERSLREFEGQALLIVNVASKCGFT
                     PQYKGLEALWRKRRDAGFAVLGFPCDQFGHQEPGDEAQIKEFCSLNYDVTFPLFAKIE
                     VNGAGAHPLYLWLKGEKAGVFGTEAIKWNFTKFLVDRSGLVVKRFAPADSPDKIDKLV
                     GDVLAGN"
     gene            1149..1550
                     /locus_tag="VFL84_10585"
     CDS             1149..1550
                     /locus_tag="VFL84_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609113.1"
                     /translation="MRMPFRALVVLAASSLALPVLAQQAAAGRWNASVDTPQGPFAFT
                     FEFVVDAAGKLTGSMINEFIGSIPIKDGVAKGNDLSFKLSIEGAPDGSVMTINYTGTV
                     KGDELALTSKFEGTPPGGGPAETKFTATRAK"
     gene            complement(1558..>2579)
                     /locus_tag="VFL84_10590"
     CDS             complement(1558..>2579)
                     /locus_tag="VFL84_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7609114.1"
                     /translation="VLALAACVVASQGAAQDSARAQDEAAREVARQEQEFAREQEALH
                     REFARQQEAFQREHVAAQRERLNQLSELSRTNALSALENADVQAQLAQAREQLAVAVG
                     QVARAAQLAQPAQYATRKGTSFVFAGGQPVLGLNIEDSDFGVFVTGVTPNGPAAKAGV
                     TVGDTIVAINDVELARIAGTRSPSAALLGQIGTIAPGEDVKLRILRGGDYRDVVVKAG
                     EGWLRQPFVFTGDRPVVRLNNWGAFFSNGQWSDIELVSLTPALGEYFGVSEGLLVVRA
                     GRASELGFRDGDVIIDIAGREPQSPEHALRILASFEPGESLQAAIMRQKKRESLAIRV
                     PTSTD"
BASE COUNT          429 a          901 c          825 g          424 t
ORIGIN      
        1 cgccgagcac gagttcaatg cctccacctt ccccgcgcgc gtgattgcag gaaccggcag
       61 cgacatgcat gcgtgcatca ccggcgcgat cggcgcgttg cgcggcccga agcacggcgg
      121 cgcgaacgag gtcgcgctcg agatccaaga acgctacgcc acgcccgagg aggcggtggc
      181 cgacatccgc gcgcgcctcg ctcgcaagga gctcgtgatc ggcttcggtc atcccgtcta
      241 cacggtcgcg gacccgcgca atcgagtcat caaggacgtc gcgcgccgcc tgtccgagga
      301 agcaggcgac ctcacgctgt ttcgcgtcgc cgatgccatc gaagcggaga tggccgccgc
      361 gaaaaaaatg ttcgcaaacc tcgattggtt ctcggccgtc gcctatcacg tcatggaggt
      421 gcccacgccc atgttcacgc cgctgttcgt gatcgcgcgc acgaccggct gggccgcgca
      481 cgtcatcgag caacgcgccg acggcaagat catccggccg agcgcgaatt acgtcgggcc
      541 cgagaaccgc cgtttcgtcc cgctcgccga gcgcggctga tcaattcccg gccagcacgt
      601 cgccgacgag cttgtcgatc ttgtcaggcg agtcggcggg cgcgaagcgc ttgacgacca
      661 agccgctgcg gtcgacgagg aacttcgtga agttccactt gatcgcctcg gtgccgaaca
      721 cgcctgcctt ctcgcccttg agccacaggt agagcggatg cgcgcccgcg ccgttgacct
      781 cgatcttcgc gaacagcgga aacgtgacgt cgtagttcag cgaacagaac tccttgatct
      841 gcgcttcgtc gccgggctct tgatgcccga actgatcgca cggaaagccg agcaccgcga
      901 acccggcgtc gcgccgcttg cgccacagcg cttcgagccc tttgtactgc ggcgtgaacc
      961 cgcacttcga ggcgacgttg acgatcagca gcgcctggcc ttcgaactcg cgcagcgagc
     1021 gctcgcggcc gtcgatgtcc attgccgtga agtcataggc ggtcttaccg ctgcttgcgt
     1081 tcatggattc gggtgcctta tatacggctc tgcacgtcgg cttcggtagc taaccaggag
     1141 aataaaccat gcgcatgccg ttccgcgccc tcgtcgtgct cgcagccagc tccctcgccc
     1201 tgccggtcct cgcgcaacaa gccgcggccg gccgctggaa cgccagcgtc gacacgccgc
     1261 aaggcccgtt cgcgttcacg ttcgagttcg tcgtcgatgc cgcagggaag ctgaccgggt
     1321 cgatgatcaa cgagttcatc ggctcgatcc cgatcaagga cggcgtcgcc aagggcaacg
     1381 acctgtcgtt caagctgtcc atcgaaggcg cgcccgacgg cagcgtcatg acgatcaact
     1441 atacgggcac cgtcaaaggc gacgagcttg cgctgacgag caaattcgaa ggcactccgc
     1501 ccggcggcgg gcccgccgag acgaagttca cggcgacgcg cgcgaagtaa gcgcggctca
     1561 gtccgtagaa gtcggcacgc ggatcgcgag cgactcgcgc ttcttctggc gcatgatcgc
     1621 ggcttgcagc gactcgccgg gctcgaagct cgcgagaatt cgcagcgcgt gctcggggct
     1681 ctgcggctcg cggcccgcga tgtcgatgat cacgtcgccg tcgcggaagc cgagctcgct
     1741 cgcgcggccc gcgcgcacga cgagcaggcc ttcgctgacg ccgaagtatt cgccgagcgc
     1801 cggcgtcagc gacacgagct cgatgtcgct ccactgtccg ttgctgaaga aggcgcccca
     1861 gttgttcagt cgcactacgg gccggtcgcc agtgaacacg aacggctgac gcagccagcc
     1921 ttcaccggct ttcacgacga cgtcgcgata gtcgccgccg cgcaaaatgc ggagcttcac
     1981 gtcctcaccc ggcgcgatgg tgccgatctg accgagcaaa gcggccgagg gcgagcgcgt
     2041 gcccgcgatg cgagcgagct cgacgtcgtt gatcgcgacg atcgtgtcgc cgacggtgac
     2101 gcccgccttg gcggccgggc cattcggcgt cacgccggtg acgaacacgc cgaagtcgct
     2161 gtcttcgatg ttgaggccga ggacgggctg gccgcccgcg aagacgaacg acgtcccctt
     2221 gcgcgtcgcg tattgtgccg gctgcgcgag ctgcgcagcg cgcgcaacct gaccgaccgc
     2281 aaccgcgagc tgctcgcgcg cctgcgcgag ctgcgcttgg acgtccgcgt tctcgagggc
     2341 cgacagcgcg ttcgtgcgcg acagctcgct cagctggttc aacctctcac gctgcgcggc
     2401 gacatgctca cgctggaacg cttcctgctg gcgcgcgaac tcgcggtgca gcgcttcttg
     2461 ctcgcgcgcg aactcttgct cttggcgcgc gacctccctc gccgcctcgt cttgcgctct
     2521 cgccgagtct tgcgcggctc cctgcgaggc gacgacgcat gcagccaaag caagcacgc
//
LOCUS       DASSNN010000571         3330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1611430, whole genome shotgun sequence.
ACCESSION   DASSNN010000571 DASSNN010000000
VERSION     DASSNN010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611430"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFL84_10595"
     CDS             complement(<1..219)
                     /locus_tag="VFL84_10595"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET7609115.1"
                     /translation="MARLLLLNGPNLGQLGRREPGLYGTTSLSDIESLLRARAVAAGH
                     ELDAYQSDSEADLIKRVHAARDQGVDLIL"
     gene            complement(303..908)
                     /locus_tag="VFL84_10600"
     CDS             complement(303..908)
                     /locus_tag="VFL84_10600"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET7609116.1"
                     /translation="MARSRPVLLTAALSAAAVLGYLSYRGLTRDVEAPAESTESAAPS
                     AVATLPDFSLANLAGEQQSIRSWPGKPLLINFWATWCGPCLREIPMLKELQASHPDLQ
                     VVGIAIDKPDLVTKFAGDMQFNYAILVGQNEAWAAAGALGVNIFALPFTVFTAGDGSI
                     LGVHTGELHAEHLEAFRDTVDALAAGRIDVPEARRRLADAI"
     gene            complement(920..3106)
                     /gene="dsbD"
                     /locus_tag="VFL84_10605"
     CDS             complement(920..3106)
                     /gene="dsbD"
                     /locus_tag="VFL84_10605"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HET7609117.1"
                     /translation="MLALLVAGAGSACAQDQGQTDELLPAEQVFRYEPTADAGQLYLD
                     FDVLDGHYLYRTRFGFESGTAGVAIGAARFPHGETHSDEFFGDQEVYRHKFRIAIPYK
                     RTAAVESFDLKVELQGCADRGLCYLPQEWTATIALPPVPFLGVGAIDASATDDQLSVD
                     EAFTMNARFDKPNELTVGWQVAPGYYLYRDKLTFAANGEIELGAAALPKGVPHTDDSF
                     GDVEIFRDYVEAKVPFARASPDELEVEIVAGFQGCKDASICYPPGEQRMTLKLPATSE
                     FAASAAPAGDELVSEQDQWAARIVNGSWPEMLGWFFFGGLLLSFTPCVLPMVPILSSI
                     IAGQGAVSTGRGFALSVSYVLGMAFTYTVLGMLSALVGKQLQAGLQEPWILSAFAGVF
                     VVLALGLFGAFQFQMPAAIQTRLSNLANQQKAGTFAGTAVIGALTSLILTTCVAPVLI
                     GALLAIGQTGDVARGGGALFAMSIGMGTPLLAVGASAGYLLPKVGPWMNTIKAGFGVV
                     MLGVAIYLLGRFLPPTPTLFLWALLVFFTGVFIGAFDPLPASPTVGRRFAKGIGALVC
                     LYGALLFVGALLGGDDVFEPIPRAALTQSGGGAVASTGSQELAFREIDTVAALDAALM
                     EARTAGQPVMLDFTADWCISCKEMEEYTFPDSSVVGALKPFMLLRADVTDNDDDDQAL
                     LQRFHSFGPPTIAFFDRSGFERTNYKLVGFVPADKFREHVTNLAAL"
     gene            complement(3144..>3330)
                     /gene="cutA"
                     /locus_tag="VFL84_10610"
     CDS             complement(3144..>3330)
                     /gene="cutA"
                     /locus_tag="VFL84_10610"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HET7609118.1"
                     /translation="VQQDQETLLIIKTCADLLAAVEKTIREKSKYELPELLALRVEAG
                     GAEYLNWIRESVAPLKD"
BASE COUNT          594 a         1090 c         1120 g          526 t
ORIGIN      
        1 caaaatcagg tctacgcctt gatcgcgcgc ggcgtgcacg cgcttgatca gatcggcttc
       61 cgaatcgctt tggtaggcgt cgagctcatg gccggcggcg acggcgcgcg cgcgcagcag
      121 cgactcgatg tcgctcaagg aagtcgttcc gtagaggccg ggctcacggc ggccgagctg
      181 gcccagattc gggccattca gtaacagcag gcgagccatg gcggcgcgtc ccccacgcgg
      241 tcgatggcgc gagtttaacc ggttttcggc gcaattggcg ccactttcgg aatagaacgg
      301 ctctagatcg cgtcggcgag gcggcggcgg gcttccggaa cgtcgatgcg gccggccgcc
      361 agggcgtcga ccgtgtcgcg gaacgcctcg aggtgctcgg cgtgcagctc gccggtatgg
      421 acgccgagga tgctgccgtc gccggccgtg aagaccgtga acggcagggc gaagatgttg
      481 acgccgagcg ccccggcggc ggcccaggcc tcgttctggc cgaccaggat cgcgtagttg
      541 aactgcatgt cgccggcgaa cttcgtgacg aggtccggct tgtcgatcgc gatgccgacg
      601 acttgcagat cggggtgcga cgcctgcagc tccttcagca tcggaatctc gcgcaggcac
      661 ggcccgcacc aggtagccca aaagttgatc aacagcggct tgccgggcca gctgcggatc
      721 gactgctgct cgccggccag gttcgcgagc gagaagtcgg gcagcgtcgc gacggccgac
      781 ggggccgcag actcggtgct ttcggcgggc gcctcgacgt cgcgcgtcaa gccgcgatag
      841 ctcaaatagc cgagaacggc cgcggcgctc agcgccgcgg tcagcagcac ggggcgcgac
      901 ctagccatga aacgactccc tacagcgccg cgaggttcgt gacgtgctcg cggaatttat
      961 cggccggcac gaatccgacg agcttgtagt tcgtgcgctc gaagccgctt cgatcgaaga
     1021 acgcgatcgt cggcggcccg aagctgtgaa agcgctgaag caatgcttgg tcatcgtcgt
     1081 cgttgtcggt tacgtcggct cgcaacagca tgaacggttt cagcgcgccg acgacggagg
     1141 aatcgggaaa cgtgtactcc tccatctcct tgcaggaaat acaccagtct gccgtgaagt
     1201 ctagcattac gggctgaccc gccgtccgcg cttccatgag cgcggcgtcg agcgctgcga
     1261 ccgtgtcgat ctcgcggaac gcaagctcct gtgatcccgt ggacgcgacg gcccctccgc
     1321 cgctctgcgt cagggcggcg cgcgggatcg gctcgaaaac gtcgtcgccg ccgagcaacg
     1381 cgccgacgaa cagcaacgct ccgtacaggc agaccagcgc gccgatgccc ttggcgaagc
     1441 ggcgcccgac cgttgggctc gccggcagcg gatcgaacgc gccgatgaag accccggtga
     1501 agaacacgag cagcgcccac aggaacagcg tcggcgtcgg cggcaggaag cgtccgagca
     1561 ggtagatcgc gacgccgagc atgacgacgc cgaagccggc cttgatcgtg ttcatccacg
     1621 ggccgacctt gggcagcagg taaccggcgg atgccccgac ggcgagcagc ggcgtcccca
     1681 tcccgatgct catcgcgaac agcgcgccgc cgccgcgcgc cacgtcgccc gtttgcccga
     1741 tcgcgagcag ggctccgatc agcaccggcg cgacacaggt cgtgaggatc agcgatgtca
     1801 gagcgccgat cacggccgtg cccgcgaacg tcccggcctt ctgctgattc gcgaggttcg
     1861 atagccgcgt ttggatcgcg gccggcatct ggaactggaa tgcgccgaac agcccgagcg
     1921 cgagcacgac aaaaacgccc gcgaatgcgg acaagatcca cggttcctgc aggcccgcct
     1981 gcaactgttt gccgacgagg gccgacagca ttccgagaac cgtgtacgtg aacgccatgc
     2041 cgagcacata gctgaccgag agcgcgaagc cgcggcccgt cgacacggcg ccttggccgg
     2101 cgatgatgct cgacaggatc ggcaccatcg gcagcacgca cggcgtgaac gacaacagca
     2161 ggccgccgaa gaagaaccag ccgagcatct ccggccacga gccgttgacg atgcgcgccg
     2221 cccattggtc ttgctcggag acgagctcgt cgcctgccgg cgcggcgctc gcggcgaact
     2281 cgctcgtcgc cggcagcttc agtgtcatgc gctgctcgcc gggcggatag caaatgctcg
     2341 cgtctttgca gccctggaag ccggcgacga tctcgacctc gagctcgtcg gggctcgcgc
     2401 gcgcgaacgg caccttcgct tcgacgtaat cgcggaagat ttcgacgtcg ccgaaactgt
     2461 cgtcggtatg gggcacgcct ttcggcagcg ccgcagcgcc gagctcgatc tcgccgttgg
     2521 cggcgaaggt cagcttgtcg cggtagaggt agtagccggg cgcgacttgc cagccgaccg
     2581 tgagctcgtt cggcttgtcg aagcgcgcat tcatcgtgaa cgcttcatcg acgctgagct
     2641 gatcgtcggt cgccgatgcg tcgattgcgc ccacaccgag aaacggcacg ggcggaagag
     2701 cgatcgtggc cgtccactcc tggggcaggt agcagagacc gcggtccgcg catccctgta
     2761 gctcgacctt cagatcgaag ctctcgaccg cggccgtgcg cttgtaaggg atcgcgatgc
     2821 gaaacttgtg gcgatagacc tcttggtcgc cgaagaactc gtcgctgtgc gtctcgccgt
     2881 gcgggaaacg cgcggcgccg atcgcgacac cggccgtgcc gctctcgaaa ccgaagcggg
     2941 tccggtagag gtagtggccg tcgaggacgt cgaagtcgag atagagctgg cccgcatcgg
     3001 cggtcggctc atagcgaaag acctgctcgg ccggaagcag ctcgtcggtc tggccttggt
     3061 cttgcgcgca ggcgctgccg gctccggcga cgagcaacgc cagcaagact cggagtgcta
     3121 aacgcgatgc gaccatagcg gcgttagtcc tttaacgggg caacggattc ccggatccaa
     3181 ttgaggtact ccgcgccacc cgcttcgacc cgcaacgcga gcagctcggg cagctcgtac
     3241 ttcgatttct cgcgaatcgt cttctcgacg gcggcgagca ggtcggcaca cgtcttgatg
     3301 atcaacagag tctcctgatc ctgctgaact
//
LOCUS       DASSNN010000572         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_602413, whole genome shotgun sequence.
ACCESSION   DASSNN010000572 DASSNN010000000
VERSION     DASSNN010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602413"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFL84_10615"
     CDS             <1..527
                     /locus_tag="VFL84_10615"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7609119.1"
                     /translation="TRAVLKETGAKLNLRYDASAWTQPLEELSPEAVHLTDPSRLRLE
                     AARLIVSSGRTVVAEVSLNRPRMVLGRDDSCDISLDSRYVSRYQNLFMETPEGWMLID
                     LSSTNGCFVNGRRIREHRLRDGDLIAVGHHQMRFTGPSGKARASADAAAELDSETTLV
                     GTRPEVLERSTSSH"
     gene            complement(537..1811)
                     /locus_tag="VFL84_10620"
     CDS             complement(537..1811)
                     /locus_tag="VFL84_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609120.1"
                     /translation="MDPRDSGIFRLASVGSYLDALSTITDEMSLKERSNLIFLDRCLH
                     ERWGLGENVILNWCPAGEGISLLIVPHYAIAGYTSAPSDASGRGAQSRPKQEFFQVLL
                     SGRRCLTPAQLAKVGRLLGIEPTHLSLREPLKNTPAQNEIIEKMVKRYSITYVPNRGV
                     CLFDIVGFGLLPPFEQMMQLNSLSYSLNSAQSKLLTKRIGVDFSRTTTGDGFYIWNRD
                     LSLEGNINLYHFLQLVLADNAIAQKKATHNTVPRLRACFHVGSSYEFHQSEGLNPTLY
                     NYVVGDVTVDVARVIERAMPGQILVGDFRAEMPIGDALGGGSRIVESADFVELAMRNV
                     EQLAGIELSGERVDAIRCYLTGTKLANGQFTVRRITVNDKHGIARRVYNAKVNIYRHN
                     AEPILLGIEDRSLGPDGMPGFGSEHVLRPALA"
     gene            2003..>2433
                     /locus_tag="VFL84_10625"
     CDS             2003..>2433
                     /locus_tag="VFL84_10625"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010548238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET7609121.1"
                     /translation="MANQDRDPPRVDPRRHSRVVVDGLTQAPSRAMLRAVGFKDEDFT
                     KPQVGIASTWNMVTPCNMHIDGLARHASAGADGAGAKSVLFNTITVSDGIANGTPGMR
                     YSLVSREVIADSIETVASAEGFDGLVTIGGCDKNMPGCVMA"
BASE COUNT          416 a          784 c          813 g          420 t
ORIGIN      
        1 tcacgcgagc ggtgctgaag gaaaccggcg ccaaattgaa ccttcgctac gacgcgtcgg
       61 cctggaccca accgctcgaa gagctgtcgc cggaggccgt tcacctgacg gatccgagcc
      121 ggctgcggct ggaagccgcg cggctgatcg tctcgagcgg caggaccgtc gtcgccgagg
      181 tatcgctgaa ccggccgcgc atggtgctcg gccgcgacga tagctgcgac atcagcctcg
      241 acagccgcta cgtgagccgg taccagaatc tgttcatgga gacgccggaa ggctggatgc
      301 tgatcgactt gagcagcacg aacggctgct tcgtcaacgg ccggcgaatc cgcgagcacc
      361 gcttgcgcga cggcgacctc atcgccgtcg gccatcatca gatgcgcttc acgggaccga
      421 gcggcaaggc ccgcgcgagc gccgacgcgg ccgcggagct cgattccgag acgacgctgg
      481 tcggcactcg ccccgaggtt ctcgagcgga gcactagctc acactgatcg gcccgctcag
      541 gcgagcgccg gccgcagcac gtgctcgctg ccgaaccccg gcatgccgtc ggggccgagc
      601 gagcggtcct cgattccgag caggatcggc tcggcgttgt gccgatagat gttgaccttg
      661 gcgttgtaga cgcgccgcgc gatgccgtgc ttgtcgttga cggtgatcct gcgaaccgtg
      721 aactggccgt tcgccagttt cgtgccggtc aagtagcagc ggatcgcgtc gacgcgctcg
      781 cccgagagct cgattcccgc gagctgctcg acattgcgca tggcgagctc gacgaagtcc
      841 gccgactcga cgatgcgcga gccgccgccg agcgcgtccc cgataggcat ctcggctcgg
      901 aaatcgccga cgaggatttg gcccggcatc gcgcgctcga tgacgcgcgc gacgtcgacg
      961 gtcacgtcgc cgacgacgta gttgtagagc gtcgggttga gcccttccga ttggtggaat
     1021 tcgtagctgc tgccgacgtg aaagcaggcg cgaagccgcg gcacggtgtt gtgcgtggcc
     1081 ttcttctgcg cgatcgcgtt gtcggccagc acgagctgca agaagtgata gagattgatg
     1141 ttgccctcga gcgagaggtc gcggttccag atgtagaacc cgtcgcccgt cgtcgtgcgc
     1201 gaaaagtcga cgccgatgcg cttcgtcagg agcttgctct gggcggagtt cagcgagtac
     1261 gaaaggctgt tgagctgcat catctgctcg aacggcggca agaggccgaa gccgacgatg
     1321 tcgaataggc acacgccgcg gttcgggacg taggtgatgc tgtagcgctt gaccatcttc
     1381 tcgatgatct cgttctgcgc cggcgtgttc ttgagcggct cgcgcaggga cagatgcgtg
     1441 ggctcgatgc cgagcagccg cccgaccttc gcgagctgcg ccggagtcag gcagcggcgg
     1501 ccggagagca gcacttggaa gaactcctgc ttcggccgcg actgcgcgcc gcgtccggag
     1561 gcgtccgacg gcgcgctcgt atagcccgcg atcgcgtagt gcggaacgat cagcagcgag
     1621 atgccttcgc cggccgggca ccagttcaag atcacgttct cgccgagccc ccagcgctcg
     1681 tgcaggcagc ggtcgagaaa gatcaggttg ctgcgctcct tcaggctcat ctcgtcggta
     1741 atcgtcgaga gcgcgtcgag gtagctcccg acgctggcca gcctgaagat tccggaatcc
     1801 ctcggatcca ttgttgttcg gtgtagaggt ggggcgatga tactctcggc ccccagaggc
     1861 ggcatagcag cgctagggcg ctgattctgc ggcctttagg cccaagtcag aggcgtcatt
     1921 cgcggacgcc tcggcggttg cccgaccgta tctccaccgc cataatcgcg tccccgtaaa
     1981 cggccgcacc agttcctccc ccatggcaaa ccaggaccga gatcccccgc gtgtcgatcc
     2041 gcgccgccat tcgcgcgtcg tcgtcgatgg gctcacgcag gcgcctagcc gcgccatgtt
     2101 gcgcgccgtg ggcttcaaag acgaggactt tacgaaaccc caggtcggaa tcgcgtcgac
     2161 ctggaatatg gtaacgccgt gcaacatgca catcgacggg ttggcccgcc acgccagcgc
     2221 cggcgccgat ggggcaggcg cgaaaagcgt tttgttcaat acgatcacgg tctccgacgg
     2281 catcgccaac ggcacgcccg gcatgcgcta ttcgctcgtt tcgcgcgagg tcatcgcgga
     2341 ctcgatcgag accgtggcgt cggccgaggg cttcgacggc ctcgtcacga tcggcggttg
     2401 cgacaagaac atgccgggct gcgtcatggc cat
//
LOCUS       DASSNN010000573         3686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_839680, whole genome shotgun sequence.
ACCESSION   DASSNN010000573 DASSNN010000000
VERSION     DASSNN010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839680"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFL84_10630"
     CDS             complement(<1..373)
                     /locus_tag="VFL84_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HET7609122.1"
                     /translation="MTWTVHKFGGTSVADASCIRRVAGIIGGQDRGNNLAVVVSAMKG
                     VTDDLLGLVDKAAKRQPVEATLQALRARHEKANVELLGAAGAKPIMEAFDKDLADIES
                     ILKALALVRAASNRSRDLVSGY"
     gene            complement(457..1128)
                     /gene="rpe"
                     /locus_tag="VFL84_10635"
     CDS             complement(457..1128)
                     /gene="rpe"
                     /locus_tag="VFL84_10635"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET7609123.1"
                     /translation="MRDFWIAPSILSADFARLGEEVDAVVAAGADLIHFDVMDNHYVP
                     NLTVGPAVCEALRKHGLETPIDVHLMIKPVDRIVPDFAAAGATYISFHPEATEHVDRT
                     LQLIREHGCKSGLVFNPATPLDWLDYVLDKVDLVLLMSVNPGFAGQQFIESVLPKIVA
                     VRALIDRSKLPVRLEVDGGIKVANIGRIAAAGADTFVAGSAIFGAPDYAATIAAMRRE
                     VAKPT"
     gene            complement(1121..2497)
                     /locus_tag="VFL84_10640"
     CDS             complement(1121..2497)
                     /locus_tag="VFL84_10640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7609124.1"
                     /translation="MIAARERATLAVLFVGVLLAALDIAIVGPALPAIQATFDVAPRW
                     LPTVFSVYVLFYLVGTPLLAKRSDRYGRRRVFLESLAVFGVGSLVVAAAWSFPVLLLG
                     RAVQAFGAGGLLPVAAAVIAETVPLERRGRTLGLIGAVFGVAFLLGPLLGGLLLGSSW
                     RWLFVINVPVAFVLMGAATRVLPRTGGREAGRFDAVGSALLAVVLAAIVIGIGQIDTN
                     APAASLQSLRVWPCFVLIALGIPLLWNVEKRAADPVLPPALLRSPQLRVVGVIAVAVG
                     AVEAGMVFLPAMAVLNFGIDEKTASLTMLPLVLTLAVGAPLAGWLLDHIGARSVVQLG
                     LALTVTGLLLFGLKPLDWPSFYLSGALVGFGMSALLGAPLRYIALHEAGEERRGAGQG
                     LLTLCVSVGQLVGAAIIGGVVGSASQALLGFRHSLLAVAAACALALVLSAALRGRVTN
                     EPGARANA"
     gene            complement(2498..3019)
                     /locus_tag="VFL84_10645"
     CDS             complement(2498..3019)
                     /locus_tag="VFL84_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7609125.1"
                     /translation="MLLSIGARQFASRGIDPVSVEELIAEADVSRATFYGLFSSKYSL
                     LEGILNPIFELATERIRALAARPPEEAIDGLVDIYWSLWTTHRDGLMLIPLVDHETFA
                     HFEEQHRALRQALLELLLGVERADLLRNGSAQYSLKVIARTAVQLLRVYDGHAAAAAL
                     FRDAVRGLLLRAH"
     gene            complement(3186..>3686)
                     /locus_tag="VFL84_10650"
     CDS             complement(3186..>3686)
                     /locus_tag="VFL84_10650"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7609126.1"
                     /translation="YTGGFAIACLLARARRATLIDSSPDALKLAEREAASNGVAEKCR
                     FVAANVFDELRLVRDAGTRFDLVVLDPPKFVHTADQVTAGSRGYKDINMLGLALVRPG
                     GVLATFSCSGHVDAALFQKIVAGAAVDAGSTAQILERLSQPPDHPVATEFPEADYLKG
                     LVLRVH"
BASE COUNT          667 a         1248 c         1256 g          515 t
ORIGIN      
        1 cgtacccgga gacgaggtcg cggctgcgat tcgacgccgc gcgcacgagc gcgagcgcct
       61 tcaggatgct ctcgatgtcg gcgaggtcct tgtcgaaggc ctccatgatc ggctttgcgc
      121 cggccgcgcc gagcagctcg acgttcgcct tctcgtgccg ggcccgcagc gcttgcaacg
      181 tcgcttcgac gggctgacgc ttcgccgctt tgtcgacgag gccgagcaag tcgtccgtaa
      241 cgcctttcat cgccgagacg acgaccgcca agttgttgcc gcggtcctgg ccgccgatga
      301 tgccggccac gcggcgaatg cagctcgcgt cggcaacact ggtgccgccg aacttgtgca
      361 cagtccaagt catgagtctt aacttccggg gaagcggttt tcggcggccg cgagcatagc
      421 agaacggccg tatgtttcga ggggcgggcc gcgcgtctac gtgggcttag cgacctctcg
      481 gcgcatcgcg gcgatcgtgg cggcatagtc gggcgcgccg aaaatcgccg agcccgcgac
      541 gaacgtatcg gcgccggcgg ccgcgatccg gccgatgttc gcgaccttga tgcctccgtc
      601 gacctcgagc cgcaccggca gcttcgagcg gtcgatcagc gcgcggacgg cgacgatctt
      661 cggcagcacc gactcgatga attgctggcc ggcgaaaccg gggttcaccg acatcagcag
      721 cacgaggtcg accttgtcga gcacgtagtc gagccagtcg agcggcgtcg cgggattgaa
      781 cacgagcccg ctcttgcagc cgtgctcgcg gatcaattgc agcgtgcggt cgacgtgctc
      841 tgtggcttcg ggatggaagc tgatgtaggt ggcgccggcc gccgcgaaat ccggaacgat
      901 gcgatcgacg ggcttgatca tgagatgcac gtcgatcggc gtctcgaggc cgtgcttgcg
      961 caacgcctcg cagacggccg ggcccacggt gagattcggg acgtaatggt tgtccatcac
     1021 gtcgaagtgg atcagatcgg cgccggcggc gacgacggcg tcgacctcct cgccgagccg
     1081 agcgaagtcg gccgacaaga tcgacggggc tatccaaaaa tcacgcattg gctcgcgctc
     1141 cgggctcgtt cgtcactcta ccccgcaacg ccgcgctgag caccaatgcc agggcgcaag
     1201 ccgcggcgac cgccaacagc gagtgtctga acccaagcaa cgcctggctc gccgagccga
     1261 cgacgccgcc gatgatcgcg gcgccgacga gctggcccac gctcacgcac agcgtcaaga
     1321 gcccctggcc cgcgccgcgg cgctcctcac ccgcctcgtg cagcgcgatg tagcgcagcg
     1381 gcgcgccgag cagcgcggac atgccgaagc ccaccaacgc tccggacagg tagaagctcg
     1441 gccagtcgag cggcttgagg ccgaacagca aaagacccgt gaccgtcagc gcgaggccga
     1501 gctgcaccac gctgcgcgcg ccgatgtgat cgagcagcca gcctgcgagc ggcgcgccga
     1561 ccgcgagcgt gagcacgagc ggcagcatcg tgaggctcgc cgtcttctcg tcgatgccga
     1621 agttcagcac ggccatggcc ggcaggaaca ccatgcccgc ttccactgcg ccgacggcga
     1681 cggcaatgac accgacgacg cgcagctgcg gcgagcgcaa cagcgcgggc ggcagcaccg
     1741 gatcggcggc gcgtttctcg acgttccata gcagcggaat gccgagcgcg atcaggacga
     1801 agcatggcca cacgcgcaac gactgcaagc tcgcggccgg cgcgttcgtg tcgatctggc
     1861 cgatcccgat gacgatcgca gccagcacga cggcgagcag agcgctgccc acggcgtcga
     1921 accggcccgc ttcgcggccg ccggtgcgcg gcagcacgcg agtggccgcg cccatcagca
     1981 cgaacgcgac gggcacgttg atgacgaaca gccagcgcca gctcgagccg agcagcaggc
     2041 cgccgagcag cgggccgagc aagaatgcga cgccgaacac ggcgccgatg aggccgagcg
     2101 tccggccgcg ccgctcgagc ggcacggtct cggcgatcac ggcagcggcg acgggcagca
     2161 agccgcccgc gccgaacgcc tgcacggcgc ggccgagcag cagcaccggg aacgaccacg
     2221 ccgcggcgac gaccaacgag ccgacaccga acacggcgag actttcgagg aacacgcggc
     2281 ggcgcccgta acggtccgaa cgcttcgcga gcagcggcgt gccgacgaga tagaacagca
     2341 cgtagacgct gaagacggtc ggcagccagc gcggcgcgac gtcgaacgtg gcctgaatcg
     2401 ccggcagcgc gggcccgacg atcgcgatgt cgagcgcggc cagcaaaacg ccgacgaaca
     2461 gcacggccag cgtggcccgc tcgcgcgccg cgatcatcta gtgcgcccgc agcagcaggc
     2521 cgcgcacggc gtcgcggaac agcgccgcgg cggcggcatg accgtcatag acgcgcagca
     2581 gttgcacggc cgttcgcgcg atgaccttca acgagtactg cgcgctgccg ttgcgcagca
     2641 gatcggcgcg ctcgacgccg agcagcagct cgagcagcgc ctgcctgagc gcgcggtgct
     2701 gctcctcgaa atgcgcgaac gtctcgtggt cgacgagcgg aatcagcatc agtccgtcgc
     2761 gatgcgtcgt ccaaagcgac cagtagatgt cgacgagccc gtcgatggcc tcttcgggcg
     2821 gccgcgccgc gagcgcgcga atgcgctcgg tagcgagctc aaatataggg ttaagtatcc
     2881 cttctagaag gctgtattta cttgaaaata agccgtagaa cgtggccctt gatacatccg
     2941 cctctgcaat gagctcctcg acgctgacgg ggtcgatgcc gcgcgaagcg aactggcggg
     3001 cgccgatcga gagcagcacg gcgcgcgtgc gctcgcggcg ccgctggact cgcgaagaac
     3061 gaaccgatct atttccctcg gaaaatagat ccgtcccctt tttcgccgaa ggcgctgggt
     3121 gccgcggttt catggcgaaa tcatacacga ctatctgttc ttgaacggcc gtatctggcc
     3181 ttgcgtcagt ggacgcgcag gaccaggcct ttgaggtagt cggcttccgg gaactcggtc
     3241 gcgacggggt gatcgggcgg ctgcgacaga cgctcgagga tttgtgccgt gctgcccgcg
     3301 tcgacggcag ctcccgcgac gattttctgg aacagcgccg cgtcgacgtg accggagcag
     3361 gaaaaggtcg cgagcacgcc gcccggccgg acgagcgcga ggccgagcat gttgatgtcc
     3421 ttgtacccgc ggctgccggc cgtcacttga tcggccgtgt gaacgaactt cggcggatcg
     3481 agcacgacga gatcgaagcg cgtgcccgcg tcgcgcacga ggcgtagctc gtcgaacacg
     3541 ttcgcggcga cgaatcgaca cttctcggcg acgccgttcg atgccgcctc gcgctccgcg
     3601 agcttcagcg catcgggcga cgagtcgatc aacgtcgcgc gacgcgcgcg ggccagcaag
     3661 caagcgatcg cgaatccgcc cgtgta
//
LOCUS       DASSNN010000574         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_839927, whole genome shotgun sequence.
ACCESSION   DASSNN010000574 DASSNN010000000
VERSION     DASSNN010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839927"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="VFL84_10655"
     CDS             <1..1229
                     /locus_tag="VFL84_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET7609127.1"
                     /translation="IDGFGRAAAVQVISGHQADTLATVWRHLSPARIDEIVPELPEKL
                     LKQALHALDPGVLAISLPRISEESRTRCLALLPRRAEAEVRRLMDYPAGSAGRVMDKR
                     IPSFRGEQPAALTLEQLRQSKPKDLRQLFIVDAERRLVGIVDIPDLMIAEPTDMLKEI
                     ARPASATVSPFDDREEVAQKLRDFQLDSLPVIDIHGRLIGAIRHDALIKTLEEAASVD
                     IQMMVGAGREERALSEPVFAVRKRLPWLLINLGTAAMAATVVGLFESTIAQFTALAIL
                     MPMVAGESGNTGAQALAVTMRGLALREISTRHWFRVLFKEARVGIINGLAIAATTAAG
                     VLIWSRNSGLAVVICIAMVTSMVIAGVAGAVIPIALTRLGQDPAQSSTIVLTTVTDVT
                     GFLSFLGIATLLSTLL"
     gene            1238..>2307
                     /locus_tag="VFL84_10660"
     CDS             1238..>2307
                     /locus_tag="VFL84_10660"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HET7609128.1"
                     /translation="MRLVKIALVCLAAFVVLLVAALGIVAATFDPNDYKGVAAEAFTA
                     RTGRRLTVDEDLRLTYFPWLAVETGGVTIGSAPELGGEAQPFATARRVAARVKLLPLL
                     SRRVEIGTVELEGLTLNLARDASLRGNWEDLLAAANAPAPAEPDTEPGAASIDGLAIE
                     GVRIRDGNVYWRENTNELRYSVTGWSLTTGGIGSGEPIAFETSLNFADETSGSKAALA
                     TSAVVAAAASGSVAATDLETTIELDLGDGSPVRELAAQAERIAFDRAAETLAVEGLVT
                     EAAGARAEWQVSGTSLSSNPTLSGSVAAEAANVATVLEQLRVALPASVDARELGALQA
                     HAQFSFQMEPQVVRLTGIEAAAL"
BASE COUNT          335 a          809 c          796 g          367 t
ORIGIN      
        1 tcatcgacgg cttcgggcgc gcggccgccg tgcaggttat ctccggacat caggccgaca
       61 cgctcgcgac cgtctggcgg cacctctcgc cggcgcggat cgacgagatc gtcccggagc
      121 tgcccgagaa gctgctgaag caggcgctgc acgcgctcga ccccggcgtg ctcgcgatct
      181 cgctgccgcg gatctccgaa gagtcgcgca cgcgctgtct cgcgctgctg ccgcggcgcg
      241 cggaggccga ggttcgccgg ctcatggact atccggcggg ctcggcgggc cgcgtcatgg
      301 acaagcgcat cccgagcttt cgcggcgagc agcccgccgc gttgacgctc gagcagctcc
      361 ggcagagcaa gccgaaggat ttgcgccagc tcttcatcgt cgacgccgag cggcggctcg
      421 tcggcatcgt cgacattccc gacctcatga tcgccgagcc caccgacatg ctgaaggaga
      481 ttgcgcggcc cgcgagcgcc accgtctcgc cgttcgacga ccgcgaggaa gtcgcgcaga
      541 agctgcgcga cttccagctc gactcgttgc ccgtcatcga catccacggg cggctgatcg
      601 gcgcgatccg ccacgacgcg ttgatcaaga ccctcgagga agcggcctcc gtcgacatcc
      661 agatgatggt aggcgccggc cgcgaggagc gcgcgctgtc ggagcccgtg ttcgccgtgc
      721 gcaagcgctt gccatggctg ctcatcaacc tcgggacggc ggcgatggcc gcgaccgtcg
      781 tgggcctgtt cgagtcgacg atcgcgcagt tcacggcgct cgccatattg atgcccatgg
      841 tggccggcga gtcgggcaac accggcgcgc aagcgctggc cgtgacgatg cgcggtctcg
      901 ctctgcgtga gatctcgacg cgccactggt tccgcgtgct cttcaaggag gcgcgcgtcg
      961 gcatcatcaa cggcctcgcg atcgcggcga cgacggcggc cggcgtgctg atctggagcc
     1021 ggaactcggg cctcgccgtc gtgatctgca tcgccatggt cacgtccatg gtcatcgccg
     1081 gcgtcgccgg cgcggtcatc ccgatcgccc tcacgcgctt gggtcaggac ccggctcaat
     1141 cgtcgacgat cgtcttgacg acggtcaccg acgtgaccgg cttcttgtcg ttcctcggca
     1201 ttgcaacact gctttcgacg ctgctgtagg aggagccatg cgtctcgtga agattgcgct
     1261 cgtttgcctc gccgcattcg tcgtgctgct cgtcgccgcg ctcggcatcg tcgccgcgac
     1321 gttcgatccg aacgattaca agggcgttgc ggccgaggcg ttcacggcgc gcacgggccg
     1381 tcggctcacg gtcgacgaag accttcgcct cacctacttc ccgtggctcg ccgtcgagac
     1441 gggcggcgtg acgatcggca gcgcgcccga gctcggcggc gaggcacagc cgttcgcgac
     1501 ggcacggcgc gtcgccgcgc gcgtgaagct gctgccgctc ctgtcgcgcc gcgtcgaaat
     1561 cggcaccgtc gagctcgaag gcttgacctt gaatctcgcg cgcgacgcga gcttgcgcgg
     1621 caactgggaa gacctgctcg cggccgcgaa cgcgccggcg cccgcggaac cggataccga
     1681 gcccggcgcc gcgagcatcg atgggctcgc gatcgaaggc gtgcggattc gcgacggcaa
     1741 cgtctactgg cgcgagaaca cgaacgagct gcgctacagc gtcaccggct ggtcgttgac
     1801 gacgggcggc atcggcagcg gcgagccgat cgcattcgaa acatcgttga atttcgccga
     1861 cgagacgtcc gggtcgaagg cggccctcgc gacgagcgcc gtcgttgcgg ccgcggcgag
     1921 cggctcggtt gcggcgaccg acctcgagac gacgatcgag ctcgacttgg gcgacggctc
     1981 gccggtgcgc gagctcgccg cgcaggccga gcgcatcgcg ttcgaccgcg ccgcggagac
     2041 gctcgccgtc gagggcctcg tcaccgaggc cgcgggcgcg cgagccgagt ggcaagtttc
     2101 gggcacgagc cttagctcga atcccacgct ttccggcagc gtcgccgcgg aagccgcgaa
     2161 cgttgcgacg gtgctcgagc agctgcgcgt tgctctgcct gcttccgtcg atgcgcgcga
     2221 gctcggcgcg ctgcaagccc acgcacagtt ctcgtttcag atggagccgc aggtcgtgcg
     2281 cctgaccggc atcgaagccg ccgcgct
//
LOCUS       DASSNN010000575         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_332740, whole genome shotgun sequence.
ACCESSION   DASSNN010000575 DASSNN010000000
VERSION     DASSNN010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332740"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VFL84_10665"
     CDS             <1..1159
                     /locus_tag="VFL84_10665"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HET7609129.1"
                     /translation="RLRTLAPKDSAIRRVHVVGAGTMGGDIAAWCALKGLDVTVQDRA
                     RQYVEPALKRAAELFRKRLRAPGEAEAAAQRLRVDLEAAEVGSADLVVEAIVEKAEAK
                     RALFAELEPRLPASALIATNTSSIQLENLAGALRDPGRFVGLHFFNPVASLPLVEVIR
                     GEQTSERAVLAAASFVTRIGKLPLPCRSSPGFVVNRLLTPYMLEALHAHSDGHSLESI
                     DAAAKEFGMPMGPVELADRVGLDVALHVAEILSGVLRSAPPEALRAKVAKGELGVKTR
                     RGFYAYDARDKPIKDRSRSRFDPDLPDRLLLPLVNEAVACLHEGVAADADLIDAGVIF
                     GAGFAPFTGGPLRYARARGVDDVVARLTDFARRFGTRFTPHEGWQKLRESA"
     gene            1250..>2166
                     /locus_tag="VFL84_10670"
     CDS             1250..>2166
                     /locus_tag="VFL84_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7609130.1"
                     /translation="MTKQVDSAQLKAFSPIDSLKKDNLAALAKKTKVRDAQPGETLFR
                     EGDAEKRTFYVLSGTIELREGNQRSGRITGGTEDARNPLSPKLPRRHTAIATSAVEYI
                     TIDSDLLDVMLTWDQTGSYEVSELRGESSGSDDWMTTLLQTKAFHKIPPANIQAIFMR
                     LQQVNHKAGDIVIKQGDEGDYFYVITRGKCVVTRETPLNKEGIRLAELQVGDTFGEEA
                     LISEAKRNATVAMETDGSLMRLGKEDFRKLLNEPMLDWVDAAGAEKVIQEGGQWLDVR
                     LPSEFEAYHNEGAINVPLYFIRLKLKTLDS"
BASE COUNT          392 a          711 c          722 g          341 t
ORIGIN      
        1 gcggttgcgc accctggcgc cgaaggacag cgcgatccgc cgcgtgcacg tcgtcggcgc
       61 gggcacgatg ggcggcgaca tcgccgcatg gtgcgcgctg aaaggcctcg acgtgacggt
      121 gcaggatcgc gcgcggcaat acgtcgagcc ggcgctcaag cgcgccgccg agctgtttcg
      181 caagcgcctg cgcgcgcccg gtgaagccga agccgcggcg cagcgcttgc gagtggatct
      241 cgaggcggcc gaggtcggct ccgcggatct cgtcgtggaa gcgatcgtcg agaaagcaga
      301 ggcgaaacgc gcgctgttcg ccgagctcga gccccgcttg ccggcctcgg cgctgatcgc
      361 gacgaatacc tcgagcattc agctcgaaaa tctcgccggc gccctgcgcg atccgggcag
      421 gttcgtcggg ctgcatttct tcaatcccgt ggcgagcctg ccgctagtcg aggtcatacg
      481 cggcgagcag acgtccgagc gagccgtgct ggcggcggcc tcgttcgtga cgcgcatcgg
      541 caagctgccg ctgccctgcc gtagctcgcc ggggttcgtc gtcaatcgct tgctcacgcc
      601 gtacatgctc gaggcgctgc acgcgcactc cgacggccac agcctcgaga gcatcgacgc
      661 cgcagcgaag gaattcggga tgccgatggg accggtcgag ctcgcggacc gggttgggct
      721 cgacgtcgcg ttgcacgtcg ccgaaatctt gagcggcgtg ctgcgctccg ctccgcccga
      781 ggcgctgcgc gccaaggtcg ccaaaggcga gctcggtgtc aaaacgcgtc ggggcttcta
      841 tgcgtacgac gcgcgcgaca aaccgatcaa ggaccgtagc cggagccgtt tcgacccgga
      901 ccttcccgac cggctcctgt tgccgctcgt caacgaagcg gtcgcttgcc tgcacgaagg
      961 cgtcgcggcc gatgcggacc tgatagacgc cggcgtcatc ttcggcgcgg gctttgcgcc
     1021 gttcaccggc ggcccgctcc gctatgcgcg cgcgcgcggc gtcgacgacg tcgtcgcgag
     1081 gctcacggac ttcgcgcgcc gttttgggac gcgcttcaca cctcatgagg gatggcagaa
     1141 gcttcgtgaa agcgcgtgaa accgtcggca ctggcgccgg aaggtgctgt gctagacttc
     1201 cgggcgctaa tcacttttct gcatatttgg accgcgcacc ctatccctga tgacgaagca
     1261 agtcgacagc gcccagctca aggcattttc gcccatcgac agcctcaaga aggacaacct
     1321 cgccgccctg gccaagaaga ccaaggtgcg cgatgcgcag cccggcgaga cgttgtttcg
     1381 cgagggcgac gccgagaagc gcacgttcta cgtgctctcc ggcacgatcg agctgcggga
     1441 aggcaatcag cgcagcggcc gcatcacggg cggcaccgag gacgcgcgca acccgctgtc
     1501 gccgaagctg ccgcgccggc acacggccat cgcgacgtcg gccgtcgagt acatcacgat
     1561 cgacagcgac ctgctcgacg tgatgctgac atgggaccag acgggttctt atgaagtctc
     1621 ggagctgcgc ggcgagagca gcggcagcga cgattggatg accacgctgc tgcagaccaa
     1681 ggcgttccac aagatcccgc ccgcgaacat ccaggcgatc ttcatgcgcc tacagcaggt
     1741 caaccacaag gccggcgaca tcgtcatcaa gcagggcgac gaaggcgact acttctacgt
     1801 catcacgcgc ggcaagtgcg tcgtcacgcg cgagacgccg ctcaacaagg aaggaattcg
     1861 gctggccgag ctccaggtcg gcgacacgtt cggcgaggaa gcgttgatct cggaggccaa
     1921 gcgcaacgct accgttgcga tggaaacgga cgggtcgttg atgcggctcg gcaaggaaga
     1981 ctttcgcaag cttctcaacg agccgatgct cgattgggtc gacgcggcgg gcgccgagaa
     2041 ggtcatccag gaaggcggcc agtggctcga tgtccgtcta ccgtcggaat tcgaggccta
     2101 ccacaacgaa ggtgcgatca acgtgccgct ctacttcatc cgcttgaagt tgaagaccct
     2161 ggactc
//
LOCUS       DASSNN010000576         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_332860, whole genome shotgun sequence.
ACCESSION   DASSNN010000576 DASSNN010000000
VERSION     DASSNN010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332860"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VFL84_10675"
     CDS             <1..1248
                     /locus_tag="VFL84_10675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7609131.1"
                     /translation="AELSRRGCNVSLLEARDRIGGRILTRHEPELPVPLELGAEFVHG
                     RPASTLRWLAQGGAPLIDAAQNHWLLRGGKLRPSDDLFTELKARLGKARPRHDLPFAE
                     FLAGPARRQLPPRIREFALMLVEGYDAADATRVSTLSTIEEWCGGGAADAATFRPQGG
                     YASLLRALAGALDPQRVHVRLGTIIREVRWKRGEVTIAAAQHGRAHEVVARKAIVTLP
                     LGVLQLPPQAPNGVRFVPALKAKQKALSSLAAGPVIKVLLRFRRPFWEEIDGGRYADA
                     GFFHSPRAAFPTFWTMLPARAPVLSAWAAGPQAAQLAGAGEDEIVRTALRCLAKIFAG
                     NERCLADFQGAYSHDWQADPFACGAYSYLVAGGGNAREQLARALAKTLFFAGEAAETG
                     GESGTVAGALESGKRAVEQLLAG"
     gene            1324..1707
                     /locus_tag="VFL84_10680"
     CDS             1324..1707
                     /locus_tag="VFL84_10680"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609132.1"
                     /translation="MIAKTCRWLLLSSLLLPLALYAHHSPAMFDFSKQLTLTGTVREF
                     QWTNPHSYIQLVVKPSDGAGAAQEWSLEMGANVYLYERGWRPSTVKAGDTITVTVVPL
                     RGGKTGGGLLMEAKTADGQTLGAHP"
     gene            1704..1952
                     /locus_tag="VFL84_10685"
     CDS             1704..1952
                     /locus_tag="VFL84_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609133.1"
                     /translation="MNGRRTIALASAAFVFLFCTAPFAVHAQSLPDWSGFWIGEGLRA
                     EISGFPSGGNYKLLGGDAPWSEEGRKRMTAADQRGADQ"
BASE COUNT          313 a          689 c          720 g          332 t
ORIGIN      
        1 gccgagctat cgcgccgcgg ctgcaacgtg tctctgctcg aggcacgcga ccgcatcggc
       61 ggccgcatcc tgactcgcca cgagcccgag ctgccggtgc cgctcgagct cggcgccgag
      121 ttcgtgcacg gcagacccgc atcgacattg aggtggctcg cgcaaggcgg cgcaccgctg
      181 atcgatgccg cgcagaatca ctggctcttg cgtggcggca agctgcggcc aagcgacgac
      241 ctctttaccg agctcaaggc gcggcttggc aaagcacgtc cgcgccacga tctgccgttt
      301 gccgagttcc tcgccggtcc ggcacggcgg cagctgccgc cgcgcattcg cgagttcgcg
      361 ttgatgctcg tcgaaggtta cgacgcggcg gacgcgacgc gcgtcagcac gctcagcacg
      421 atcgaggaat ggtgcggcgg cggcgccgcg gatgccgcga cgtttcggcc gcaaggcggc
      481 tatgcgtcgc tgctgcgtgc gctcgcgggc gcgctcgacc cgcagcgcgt gcacgtgcgg
      541 ctcggcacga tcattcgcga ggtgcgctgg aagcgcggcg aggtcacgat cgccgcggca
      601 cagcacggcc gcgcgcacga agtcgtcgcg cgcaaggcga tcgtcacgct gccgctcggc
      661 gttctgcagt tgccgccgca ggcgccgaac ggcgtacgct tcgtccccgc gctcaaggcc
      721 aagcagaaag cgctctcgtc gctcgcggcc gggcccgtca tcaaggtgct gctgcggttt
      781 cgccggccgt tctgggaaga gatcgacggc ggccgctacg ccgacgccgg gttcttccat
      841 tcgccgcgag ccgcgttccc gacgttctgg accatgctgc ccgcgcgcgc gccggtgctg
      901 tcggcgtggg ccgccggacc gcaggccgcg caactggcag gcgccggtga agacgagatc
      961 gttcgcacgg cgctgcgctg cctcgcgaag atcttcgccg gcaacgagcg ctgcctcgcc
     1021 gacttccagg gcgcgtactc gcacgactgg caggcggatc cgttcgcctg cggcgcctac
     1081 agctatctcg tcgcgggcgg cggcaacgcg cgcgagcagc tggcgcgggc gctcgcgaaa
     1141 acgttgttct tcgccggtga agccgcggag acgggcggcg agtcgggcac cgtcgcgggc
     1201 gcgctcgaaa gcggcaagcg cgcggtcgag cagctgctcg ccgggtagcc gtgccctgga
     1261 cgtgtaagct ttgccggcgc gattcgttat gtacgtgtct gcccacggcg ccagggggat
     1321 ctcatgatcg cgaaaacgtg tcgatggttg ttgctgtcga gcctgctctt gccgctcgcc
     1381 ctttacgctc atcattcacc ggcgatgttc gatttctcga agcagctgac gctgaccgga
     1441 accgttcgcg aattccaatg gacgaacccg cacagctaca tccagctcgt cgtcaaacca
     1501 agcgatggcg ccggcgcggc gcaagaatgg agcctcgaga tgggcgccaa cgtctatctc
     1561 tacgagcgcg gctggcgacc ttcgactgtg aaggccggag acacgatcac cgtcacggtg
     1621 gtgccgctgc gcggcgggaa aacgggtggc ggtctgctca tggaagcgaa gaccgccgat
     1681 ggtcagacgt tgggagcgca tccatgaacg gccgacggac cattgccctg gcctccgcag
     1741 cgttcgtctt cctgttctgc accgcgccgt tcgcggtgca cgcgcagtcg ttgccggact
     1801 ggagcggttt ctggatcggc gaagggctgc gggcggagat ctcggggttc ccgtccggcg
     1861 gcaactacaa gctcctgggc ggggacgccc cgtggagcga agaaggccgc aagcgcatga
     1921 ccgccgcgga ccagcgcggc gcggatcaat aggcgaaggg ctggggcgtc ccgttgatga
     1981 tgaactcggc ggcaccgctg caatttctcg tcacgccgcg cgaaacgttg atgatcaacg
     2041 tctatcgcga cgtg
//
LOCUS       DASSNN010000577         3819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1700809, whole genome shotgun sequence.
ACCESSION   DASSNN010000577 DASSNN010000000
VERSION     DASSNN010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700809"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFL84_10690"
     CDS             <1..563
                     /locus_tag="VFL84_10690"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7609134.1"
                     /translation="ADVLAFVQTASASNAARRAGLIATLLTRHRRNLLWYLMRLIRNR
                     DDAEDIAQEAFARLLGVPHLDADPSRARNYLFATATNLARDNYRRRTARAESAHVPLD
                     DLQLEATDPKPERLVDAERGCRIVDGALRDLPARSRQAFVLYVHEQMTYERIALVLGV
                     SKKTIERDVALTVALCRSRLTRWTKT"
     gene            577..1431
                     /locus_tag="VFL84_10695"
     CDS             577..1431
                     /locus_tag="VFL84_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609135.1"
                     /translation="MDRALGLALKAGRAAFVAAWLACVGSTATAQTNLIGYWNPLFHE
                     DVDERIPGPPIGDYLGLPITDAARLRAEAWDASLLSMPEHQCKPHPSTYGFRGVGVLR
                     IWEDRDEDTQQLVKIHTHIAWQEQHREIWMDGRPHPPEYAQHTWQGFSTGSWDGDVLV
                     VKTTHLKAGWMRRNGLPLSDRATMTDRFHRYGDIMTHVMIVEDPVYLTEPLVKTNGFL
                     LQPAGTMTPYPCEPVIEVVREPGFVPHYLPGTNPFLREFGERYGLPREATRGGAATAL
                     PEFAETLR"
     gene            1428..2207
                     /locus_tag="VFL84_10700"
     CDS             1428..2207
                     /locus_tag="VFL84_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609136.1"
                     /translation="MSAARFGLVLAVIASAFVGELSLGQPAPPRATTPASPRQAAPID
                     LTGQWVAIVNEDWRWRMVTPPKGDYASVPLNDEGRRVADQWDTSQDGRCEAYGAAALL
                     RMPTRLRIRWDGNARLVLETDAGRQVRNFDFGAPAAAASRSLQGQSVAKWITPPRNPG
                     GPGLAGAGGGPASTGGYLEVKTTNLTPGWLRRNGVPYSENAVVTEYFARFAAPNGDEW
                     LIVTTIVDDPRYLTQRFIVSNHFRKEAAGGHWNPRACGVGS"
     gene            2204..3157
                     /locus_tag="VFL84_10705"
     CDS             2204..3157
                     /locus_tag="VFL84_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7609137.1"
                     /translation="MSTRRLGLAALAFSFSAIVHAQTPGSDDLAVWHVRDGIYMIVGA
                     GGNTTVQVGEHGVLVVDTKLVTASDALLAAIRKISDKPIRYVINTHWHADHVGSNEAI
                     AKAGSTIAGGNVSGAIADAGEGAAVIAHENVLARMSSQKPPPPFEAWPTDTLFVPRKD
                     LYFNGEAIEILHQPAAHTDGDTLVFFRRTDVVSTGDVFTTTGYPVIRPEDGGSIDGVV
                     AALNRIIEITVPEEKQEGGTLVIPGHGRLCDEADVVEYRDMVTIIRDRIKSMVEMGMT
                     LRQVQAAKPTADYDGRYGATSGRWTTEQFVAAAYQSLKSDK"
     gene            3169..>3819
                     /locus_tag="VFL84_10710"
     CDS             3169..>3819
                     /locus_tag="VFL84_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006867419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HET7609138.1"
                     /translation="MAKGTASKSDAAKGENIGAVRCDRPQNGAEYLESLRDGREVYIY
                     GERVNDVTTHPAFRNTARMTARLFDALHDKEKSKKLLLPSDTDGKGMTHAFFKAPKTL
                     EESIAGRDAIAEWQRITYGWMGRSPDYKAAFLGTLGGNAEFYAPFEANAKRWYTFCQE
                     RVPFVNHAIIHPPVDRDKPPDKVADVCVHVDQETDGGLIISGAKVVATGSVLTNYTF"
BASE COUNT          702 a         1251 c         1265 g          601 t
ORIGIN      
        1 cagcagacgt tctggcgttc gttcaaacgg cgtccgcgag caacgcagcg cgccgggccg
       61 ggctgatcgc cacgttgctc acgcgccacc ggcgcaacct gttgtggtat ctgatgcgtt
      121 tgatccgcaa tcgcgacgac gccgaggata ttgctcagga agcgttcgcg cgcttacttg
      181 gtgtgccgca cctcgacgcc gacccgagcc gggcccgcaa ctatctgttc gcgaccgcca
      241 cgaacctcgc acgcgacaat tatcgccgcc gaacggcgcg cgccgaaagc gcgcacgtgc
      301 cgctcgacga tctgcagctc gaagctacgg acccgaagcc agagcggctc gtcgatgcgg
      361 aacgcggctg ccgaattgtc gacggagcgc tgcgcgactt gccggcgcga tcgcgtcaag
      421 cgttcgtgct atacgtacat gaacaaatga cctacgagcg cattgcgctc gtgctcggcg
      481 tcagcaagaa gacaatcgag cgcgacgtcg ccttgaccgt cgcgttgtgc cggtcgaggc
      541 ttacacgctg gacaaagacc tgaagggtcg ttgcaaatgg atagagcttt ggggctggca
      601 ttgaaagcgg gccgcgcggc cttcgtcgcc gcgtggctgg cgtgtgtggg ctcgaccgcc
      661 accgcgcaaa ccaatctcat cggatactgg aatccgttgt ttcatgagga cgtcgacgag
      721 cgcattcccg gccccccgat cggcgactac ttgggactgc cgatcaccga cgccgcgcgt
      781 ctgcgcgccg aggcgtggga tgcgtcgttg ctgtcgatgc cggagcatca gtgcaagccg
      841 catccctcga cctacggatt ccgcggcgtc ggcgtcctgc ggatctggga agaccgcgac
      901 gaagacacgc agcaactcgt caagattcac acgcacatcg cgtggcagga gcagcatcgc
      961 gagatctgga tggatggccg gcctcacccg cccgaatacg cacagcacac gtggcagggt
     1021 ttttcgacgg ggagttggga cggcgacgtg ctcgtcgtca agacgacgca cttgaaggcg
     1081 ggctggatgc ggcgcaacgg cttgccgctc agcgaccgcg cgacaatgac ggatcggttt
     1141 caccgttacg gcgacatcat gacgcacgtc atgatcgtcg aggaccccgt ctatctgacc
     1201 gagccgctcg tgaagacgaa cggcttcttg ctgcaaccgg ccggcacgat gactccgtac
     1261 ccttgcgagc ccgtgatcga ggtggtccgc gagccgggtt tcgtgccgca ctacctgccc
     1321 ggcacgaatc cattcctccg cgagttcggc gaacgctatg gtctgccgcg cgaagccaca
     1381 cgcggcggcg ccgcgaccgc gttgcctgaa tttgcggaga cgctgcgatg agcgccgctc
     1441 gtttcggtct agtgctcgcc gtcatcgctt ctgcattcgt gggtgagctc tcgctcgggc
     1501 agccggcgcc cccgcgtgcc acgacgcccg cgagcccgcg ccaagctgcg ccgatcgatc
     1561 tcacggggca gtgggtcgcg atcgtcaacg aggactggcg ctggcgcatg gtcacgccgc
     1621 cgaagggcga ctacgcgagc gtgccgctca acgacgaagg ccggcgcgtg gctgaccagt
     1681 gggacacctc gcaggatggc cggtgcgagg cctatggcgc ggccgcgctg ctgcgcatgc
     1741 ctacgcgtct gcgcattcgg tgggacggga acgcgcggct cgttctcgaa acggacgccg
     1801 gccgacaagt ccggaacttc gacttcggcg cgccggcagc tgcggcctcg cggtcgctgc
     1861 aaggtcaatc ggtggcgaag tggatcacgc cgccgcgtaa ccccggcggc cccggcctcg
     1921 caggcgccgg cggggggccc gcgagcacgg gcggctacct cgaggtcaag acgacgaatc
     1981 tgacgccggg ctggctgcgc cgtaacggcg tgccttacag cgagaacgcc gtcgtgaccg
     2041 aatacttcgc gcgcttcgcg gcgccgaacg gcgacgagtg gctcatcgtc acgacgatcg
     2101 tggacgatcc gcgttacttg acgcagcgct tcatcgtcag caaccacttc cgcaaggaag
     2161 ccgcgggcgg tcactggaat ccacgagctt gcggagtcgg gtcatgagca cgcgccgtct
     2221 cgggctcgct gccctcgcct tttcgttctc ggcgatcgtt catgcccaaa cacccgggtc
     2281 cgatgatctc gccgtctggc acgtgcgcga cggcatctac atgatcgtcg gcgcaggcgg
     2341 caacacgacg gtgcaggtcg gcgagcacgg cgtgctcgtc gtggacacga agctcgtgac
     2401 cgcatcggac gctttgctcg ctgcgattcg caagatctcc gacaagccga ttcgctacgt
     2461 catcaacacg cattggcatg ccgaccacgt cggcagcaat gaggccatcg cgaaagccgg
     2521 cagcacgatc gccggcggca acgtttccgg cgcgatcgcc gacgccggcg aaggcgcggc
     2581 ggtcatcgcg cacgagaacg tgctcgcgcg catgtcgagc caaaagccgc cgccgccttt
     2641 tgaagcgtgg ccgacggaca cgttgttcgt gccgcgcaag gacctctatt tcaacggcga
     2701 ggccatcgag atcctgcatc agccggccgc gcacaccgac ggcgacacgc tcgtgttctt
     2761 tcgccgcacc gacgtcgtca gcaccggcga cgtctttacg acgacgggtt accctgtgat
     2821 tcggccggaa gacggtggca gcatcgacgg cgtcgtcgcc gccttgaacc gcatcatcga
     2881 gatcaccgtg ccggaagaga aacaagaagg cggcacactc gtgatccccg ggcacggccg
     2941 gctgtgcgac gaagccgacg tcgtcgaata ccgcgacatg gtcacgatca tccgcgaccg
     3001 catcaagagc atggtcgaga tgggtatgac actgcggcaa gtgcaagccg caaagccgac
     3061 ggccgattac gacggccgct acggcgcaac gagcggccgc tggacgacgg agcagttcgt
     3121 cgcagcggcg taccaaagcc tgaagagcga taagtaagga gcaagcgcat ggcaaagggc
     3181 acggcgagca agagcgacgc cgcgaagggc gagaacatcg gcgcggtccg ctgcgaccgc
     3241 ccgcagaacg gcgcggagta tttggagtcg ctgcgcgacg gccgtgaggt ctacatctac
     3301 ggcgagcggg tcaacgacgt cacgacgcat ccggcgttcc gcaacacggc gcgcatgacg
     3361 gcgcggctgt tcgatgcgct gcacgacaag gagaaatcga agaagctgct gttgccgtcc
     3421 gataccgacg gcaagggcat gacgcacgcg ttcttcaagg cgccgaagac gctcgaggaa
     3481 tcgatcgcgg gccgcgacgc gatcgcggag tggcagcgca tcacctacgg ctggatgggc
     3541 cgctcgccgg actacaaggc cgcgttcctg ggcacgctcg gcggcaacgc ggagttctac
     3601 gcgccgttcg aagcgaatgc gaagcgctgg tacacgttct gccaggagcg cgtgccgttc
     3661 gtgaaccacg cgatcatcca tccgcccgtc gaccgcgaca aaccgccgga caaggtcgcc
     3721 gacgtttgcg tgcacgtcga ccaggagacc gacggcggct tgatcatctc gggtgcgaag
     3781 gtcgtcgcga cgggctcagt gctgacgaac tacacgttc
//
LOCUS       DASSNN010000578         3036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1700832, whole genome shotgun sequence.
ACCESSION   DASSNN010000578 DASSNN010000000
VERSION     DASSNN010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700832"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1054)
                     /gene="lpxK"
                     /locus_tag="VFL84_10715"
     CDS             complement(53..1054)
                     /gene="lpxK"
                     /locus_tag="VFL84_10715"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HET7609139.1"
                     /translation="MAGTAEILNGIWYGKSPMRYLLWPVAAVYRALSRLRREAYRRGW
                     RAAVEVPVPVIVVGNVSVGGTGKTPFVIWLADQLKQRGRKVGIVTRGYRGKGKEWPRA
                     VSADSDPQEVGDEPVLLARRTRCPVVAGPDRVACVRALLEDERVDVVLSDDGLQHYRL
                     ARAFEIAVVDGARGMGNGLCLPAGPLREPPSRLQEVDAIVVNGEGWGHAGVFRADAVV
                     TRVYHLKDGAERSLESFRNKPVHAVAGIGNPQRFFDLLLDADLDVDAHPLEDHAEITL
                     DELTFEEPGAVLITEKDAVKCEHLKANGVWCVVVDFKFDADSAARLMRLVLRGIGGR"
     gene            complement(1059..2831)
                     /gene="msbA"
                     /locus_tag="VFL84_10720"
     CDS             complement(1059..2831)
                     /gene="msbA"
                     /locus_tag="VFL84_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HET7609140.1"
                     /translation="MAASQSAPGVAAAMPEAELKPAETYRRLLRYVRPYWWIVAAVVV
                     PAAIYAVVGTAVPLLMGNFIDQLGDAARNSERAWVYPALIVLGFPIRGAMDVLTVYGL
                     AWVGRSVIRDLRTEVFAHYLGLPARYFDQGSSGVLISRLTYNTEMVAEAISNAVVIML
                     RDSLAVLTLLGVMIWYSPRLTLTVAVIGPVVVFVVALMSRAFRRYSARIQASMGDVTR
                     LTEQSLHGHRVVKVFEGQDHERQQFQDVNARNFRFNVRIVAVHAVGDALTQYAIALGL
                     AAVMYLSFGDLTAASFLGFITAMGMLLTPLKRLVNINSIVQRAITAAAGVFEILDQPE
                     EADTGTAPLTRARGDVAYRNVSFRYESGKERALRDVSLDVAAGTTVALVGQSGSGKTT
                     LVSLLPRFYDPDHGAVLLDGRDVREYSLRDLRRQIALVGQDVVLFDDTIANNISYGAL
                     AKFSRADVERAAEAAYVTEFAAALPRGLDTRVGERGALLSGGQRQRIAIARALLKDAP
                     VLVLDEATSALDTESERRVQAALASLMRGRTTLVVAHRLSTVERADRIVAMREGTIVE
                     SGTHAELLARGGYYASLYKMQFTV"
     gene            complement(2842..>3036)
                     /locus_tag="VFL84_10725"
     CDS             complement(2842..>3036)
                     /locus_tag="VFL84_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7609141.1"
                     /translation="KNERGTLRAAIERLTGDRRDLPVFIRADAAATHQSVVTAMDVAG
                     QLGFVKLNIATVTPPEAQAQ"
BASE COUNT          515 a         1047 c         1029 g          445 t
ORIGIN      
        1 agccgtgtcg aggcataccg cgcagggatc accgcgatga agccgtccgc cactagcgcc
       61 ctccgatgcc gcgcaggacg agccgcatga gccgcgcagc gctatcggca tcgaacttga
      121 aatcgaccac tacgcaccac acgccgttcg ccttcagatg ctcgcatttt actgcgtcct
      181 tctccgtgat cagaacggcg ccgggctcct cgaacgtcag ctcgtcgagc gtgatctcgg
      241 cgtgatcctc gagcggatgc gcatcgacgt cgagatccgc gtcgagcagc agatcgaaga
      301 atcgctgcgg attgccgatg ccggcgaccg cgtgcacggg cttgttgcga aacgactcga
      361 gggagcgctc cgcgccgtcc ttcaaatgat agaccctcgt gacgacggcg tcggcgcgga
      421 acacgccggc gtggccccag ccttcgccgt tgacgacgat ggcgtcgacc tcctgcaagc
      481 gcgacggggg ctcgcgcagc gggccggccg gcagacacag gccgttgccc atgccgcgcg
      541 cgccgtcgac gacggcgatc tcgaacgcgc gcgcgaggcg gtagtgctgc aacccgtcgt
      601 ccgacagcac gacgtcgacg cgctcgtcct cgagcagcgc cctgacgcat gcgactcggt
      661 cggggcccgc cacgacgggg cagcgcgtgc ggcgagcgag cagcacgggc tcgtcgccga
      721 cttcttgcgg gtcgctgtcg gcgctgacgg cgcgcggcca ttccttgccc ttgccgcgat
      781 agccccgcgt cacgatgccg accttgcggc cgcgctgttt caactggtcg gcgagccaga
      841 tcacgaacgg cgtcttgccc gtgccgccta cgctgacgtt gccgacgacg atgacgggaa
      901 cggggacttc gacggccgca cgccaaccgc gccgatacgc ctcgcgccgc agccgcgaca
      961 gcgcgcggta gacggccgcg accggccaca gcaagtaacg catcgggctc ttgccgtacc
     1021 agatgccatt caggatctcg gctgtcccgg ccatgcggtc agacggtgaa ctgcattttg
     1081 tagagcgagg cgtagtagcc gccgcgagcc aagagctccg cgtgcgtgcc gctctcgacg
     1141 atggtgcctt cgcgcatcgc gacgatgcga tcggcgcgct cgacggtcga aagccgatgc
     1201 gcgacgacga gcgtcgtgcg gccgcgcatc agactcgcga gcgccgcctg gacgcgccgc
     1261 tcggattccg tgtcgagcgc cgaagtcgcc tcgtcgagca cgagcacagg cgcatccttg
     1321 agcagcgcgc gcgcgatcgc gatgcgctgg cgctggccgc ccgagagcag cgcgccgcgc
     1381 tcgccgactc gcgtgtcgag gcctcgcggc agagccgccg cgaactccgt gacgtacgcg
     1441 gcctcggccg cacgctcgac gtcggcgcgc gagaatttcg cgagcgcgcc gtaagagatg
     1501 ttgttcgcga tcgtgtcgtc gaacagcacg acgtcctggc ccacgagcgc gatctgccgc
     1561 cgcaggtcgc gcagcgagta ctcgcgtaca tcgcgtccgt cgagcagcac ggcgccgtgg
     1621 tccgggtcgt agaaccgcgg cagcaggctc acgagcgtcg tcttgccgct gccggactgg
     1681 ccgacgagcg cgacggtcgt tccggctgcg acgtcgagac tcacgtcgcg caacgcgcgc
     1741 tccttgcccg actcgtaacg gaacgagacg ttgcgatacg caacatcgcc gcgcgcgcgc
     1801 gtgagcggcg cggtccccgt atcggcctcc tccggttgat cgaggatctc gaacacgccg
     1861 gccgcggccg tgatcgcacg ctgcacgatg gaattgatgt tgacgaggcg cttcaacggc
     1921 gtcagcagca tgcccatcgc ggtgatgaag ccgaggaacg acgccgcggt cagatcgccg
     1981 aacgacaggt acatgacggc cgcgaggccg agcgcgatcg cgtattgcgt cagcgcatcg
     2041 ccgacggcat gcacggccac gatgcgcacg ttgaaacgaa aattgcgcgc gttgacgtcc
     2101 tgaaactgct gccgctcgtg gtcttggcct tcgaacacct tcacgacgcg atggccgtgc
     2161 agcgactgct cggtcagccg cgtgacgtcg cccatcgagg cctggatacg cgcgctgtag
     2221 cggcgaaacg cgcggctcat caaggctacg acgaaaacca cgacggggcc gatcacggcg
     2281 acggtcagcg tgagccgcgg gctgtaccag atcatgacgc cgagcagcgt gagcaccgcg
     2341 agcgagtcgc gcagcatgat cacgacggcg ttcgagatgg cctccgcgac catctccgtg
     2401 ttgtacgtga ggcgggagat caagacgccg gacgaccctt ggtcaaagta gcgcgccggc
     2461 aagccgaggt agtgcgcgaa cacctcggta cgcaggtcgc gaatgacgga ccggccgacc
     2521 caggcgagcc catagacggt caacacgtcc atcgcgccgc gaatcggaaa tccgagaacg
     2581 atcagcgccg gatacaccca ggcgcgctcc gagttgcgcg ccgcgtcgcc gagctggtcg
     2641 atgaagttgc ccatgagcag cggcaccgcc gtgcccacga cggcgtagat ggcggccggt
     2701 acgacgacgg ccgcgacgat ccaccagtac ggacgcacat agcgaagcag gcgccgatac
     2761 gtctcggcgg gcttgagctc ggcctcgggc attgccgccg ctacaccggg tgccgattgc
     2821 gaggcggcca cgctccgcgg ctcactgcgc ttgcgcttct ggcggcgtga cggtcgcgat
     2881 gttgagcttg acgaagccga gctggcccgc gacgtccatc gccgtgacga cggactgatg
     2941 cgtcgcggcc gcgtcggcgc gaatgaacac cggcaagtcg cgcctgtcgc cggtgagccg
     3001 ctcgatcgcg gcgcgcaacg tgccgcgctc gttttt
//
LOCUS       DASSNN010000579         3114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1229995, whole genome shotgun sequence.
ACCESSION   DASSNN010000579 DASSNN010000000
VERSION     DASSNN010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229995"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..916)
                     /locus_tag="VFL84_10730"
     CDS             complement(125..916)
                     /locus_tag="VFL84_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7609142.1"
                     /translation="MADIEILELEDFALELAHTAGGIAQAHFRRPVTVENKSATAFDP
                     VTNADRAIERVLRTAIVERYPEHGIVAEEEGERAGKGEYTWFIDPIDGTRSFMTGSPL
                     WGTLIGLTRRDQPLFGLLVQPVLEEVFLGGPSGSWLIKAERRDRLKSRGCKTLAKAML
                     GCTHPDMFTGERATAFRALSGQCLLTRFGGDCYNYAMVAAGYLDLVVESQLKPYDIVP
                     LIPILEGAGCLVTDWHGKPPLRGGDVVVAGSRELHKAALAALRVA"
     gene            complement(920..2290)
                     /locus_tag="VFL84_10735"
     CDS             complement(920..2290)
                     /locus_tag="VFL84_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7609143.1"
                     /translation="MQNEGAVSAKVNLSELLENSRVGAMHIRIFVLCMACLIMDGFDV
                     QAMGYTAPAVIAEWGLERSVMGPVFVAANFGVLLGSLIFSMLADKIGRRPVIVWATVF
                     FSVMTVATAFAQNLEQLLLLRFIAGIGLGSIIPNATALIGEFSPKATRTTFVMCITVG
                     FTIGAAIGGFVAAWLIPAFGWRSVFVFGGIVPLVIALFMMWGLPESLQFLAVRKRRLD
                     LLARWLKELAPNLRVDASTQYVANETSKGGVPLWHLFREGRAVFTVLLWIVNFMNILM
                     LYSLSNWLPTLVTGMGYDQQTAVLVSTLLQVGGAIGTFGLAWIIARRGFIPVLLTSFA
                     IAAVSTALIGQQGLSLTLLSVVVFIAGWCVVGGQPGLNALSASYYPTYLRATGVGAGL
                     GVGRMGAIVGPGIAGILIGRQWGSEELFYAAAAPALISAVVMLIMRGVYKETVPAQAA
                     PAPLAH"
     gene            complement(2393..>3114)
                     /locus_tag="VFL84_10740"
     CDS             complement(2393..>3114)
                     /locus_tag="VFL84_10740"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET7609144.1"
                     /translation="VEPTPVVEERRVEPEPKPEVKPPPTPEPPPVDRTQQARERARVA
                     GLLPLTNQLQALRNNVTNERLQKTDTVAAAASSDAPFSERSLITSRVGTASGGINTAA
                     LSRNTGGSGLSGRETTQVESPVEGFAEAGGAAQRTGSSDLASRSREEIERVFDANKGR
                     IYTLYNRALRENPALQGKVVLRLTIAPDGRVTFCEIVSSELNDPELERGLVQRVLQFQ
                     FEARDDVEAITTTKPIDFFPA"
BASE COUNT          530 a         1057 c         1026 g          501 t
ORIGIN      
        1 cggtcgccgg cggttcgggg tctttgcgct gcgctcggct catattttgt caaacgggct
       61 atcgacccgg catgacgagg gtagccgaag cccgcgcgcg gctctacgaa ccggttcaca
      121 cggcttaggc gacgcgcagc gcggcaagcg ccgctttgtg cagctcgcgg ctgcccgcga
      181 cgacgacgtc tccgccgcgc aacggcggct tgccgtgcca gtcggtcacg agacagcccg
      241 cgccttcgag aatcgggatc aacgggacga tgtcgtacgg tttcagctgg ctctcgacca
      301 cgaggtcgag atagccggcc gcgaccatcg cgtagttgta gcagtcgccg ccgaaacgcg
      361 tcagcagaca ttggccgctc aacgcacgga atgccgtcgc gcgctcgccc gtgaacatgt
      421 ccgggtgcgt gcaaccgagc atcgccttcg cgagcgtctt gcagccgcgg ctcttcaaac
      481 ggtcgcgccg ctcggccttg atgagccagc tgccgctcgg cccgccgagg aacacttcct
      541 cgagcacggg ctgcacgagc aggccgaaca gcggttggtc gcggcgcgtg agccctatca
      601 gcgtgcccca gagcggcgag cccgtcatga acgagcgcgt cccgtcgatc gggtcgatga
      661 accacgtgta ctcgcccttg ccggctcgct cgccttcttc ctcggcgacg atgccgtgct
      721 cgggataacg ctcgacgatc gccgtgcgca acacgcgctc gatcgcgcgg tccgcgttcg
      781 tcacggggtc gaacgccgtt gcgctcttgt tctcgacggt gacgggccgg cggaaatgcg
      841 cctgcgcgat gccgcccgcc gtgtgtgcaa gctccagcgc aaaatcttcg agctcgagga
      901 tctcgatatc ggccatgcct cagtgcgcga gcggagccgg agccgcttgc gccggaactg
      961 tctctttgta cacgccgcgc atgatgagca tcacgacggc ggagatcagg gccggcgcgg
     1021 cggcggcgta gaacagctcc tcggaccccc actgcctgcc gatcagaatg ccggcgatgc
     1081 cggggccgac gatcgcaccc atgcggccga cgccgagacc ggctccgaca cccgtcgcgc
     1141 gcaggtaagt cgggtagtag ctcgcggaaa gggcgttgag gcccggctgt ccgccgacca
     1201 cgcaccagcc cgcaatgaac acgacgacgc tgagcagcgt cagcgacagt ccttgctggc
     1261 cgatcagcgc cgtgctcacg gcggcgatcg cgaagctcgt cagcagcacc ggaatgaagc
     1321 cgcggcgcgc gatgatccaa gcgaggccga acgtgccgat ggcgccgccg acttgcagca
     1381 gcgtgctcac gagcacggcc gtttgctggt cgtatcccat gccggtcacg agcgtcggca
     1441 gccagttcga cagcgagtac agcatcagga tgttcatgaa gttcacgatc catagcagca
     1501 ccgtgaagac ggcgcgcccc tcgcggaaca agtgccaaag cggcacgccg cccttgctcg
     1561 tctcgttcgc gacgtactgc gtgctcgcgt cgacgcgcag gttcggagcg agctccttga
     1621 gccagcgcgc gagcaagtcg aggcgccgct tgcggacggc gaggaactgc agcgactcgg
     1681 gcaagcccca catcatgaac agcgcgatca cgagcggcac tatgccgccg aagacgaaga
     1741 ccgagcgcca gccgaacgcg ggaatcagcc atgcggccac gaagccgccg atcgccgcgc
     1801 cgatcgtgaa gccgacggta atgcacatca cgaacgtcgt ccgcgtcgcc ttcgggctga
     1861 actcgccgat cagcgccgtc gcattgggga tgatcgagcc caagccgatg ccggcgatga
     1921 agcgcaacaa gagcaattgc tcgaggttct gcgcgaacgc ggtcgcgacc gtcatgacgg
     1981 agaagaaaac cgttgcccag acgatcacgg gcctgcgccc gatcttgtcg gcgagcatgc
     2041 tgaagatcag agagcccaag aggacgccga aattcgccgc gacgaatacg ggccccatca
     2101 ccgaacgttc gaggccccat tccgcgatca cggccggcgc cgtgtagccc atcgcctgaa
     2161 cgtcgaagcc atccatgatg aggcacgcca tgcagagcac gaagatgcgt atgtgcatcg
     2221 cgcctacgcg gctgttctcg agaagctcgc tgagattgac cttagcgctg acggcgcctt
     2281 cgttttgcat cgcgattccc cctgtcgact ctcggcctgc gtgccgacgg cacgcgggtc
     2341 cggccaggcc actatacagg tcggcccgca gccggtgcac aacgagaatc ttctacgcgg
     2401 ggaagaagtc gatcggcttc gtcgtcgtga tcgcctcgac gtcgtcgcgc gcctcgaatt
     2461 ggaactgcag cacgcgctgc acgaggccgc gctcgagctc gggatcgttg agctccgacg
     2521 aaacgatttc gcaaaacgtc acgcgcccgt cgggcgcgat cgtgaggcgc agcacgacct
     2581 tgccttgcag ggccgggttc tcgcgcagcg cgcgattgta gagcgtgtag atgcgcccct
     2641 tgttcgcgtc gaagacgcgc tcgatctctt cgcggctgcg cgaggccaag tcgctcgagc
     2701 cggtgcgctg cgccgcgccg cccgcctccg cgaatccttc gacggggctc tcgacttgcg
     2761 tcgtctcgcg gccgcttaag ccgctgccgc cggtgttgcg gctcaacgcc gcggtgttga
     2821 taccgccgct cgccgtgccg acgcgcgacg tgatcagaga acgttccgag aacggggcgt
     2881 cgctgctcgc agccgcggca acggtatccg tcttctgcag ccgttcgttc gtgacgttgt
     2941 tgcgcagcgc ctgcagctga ttcgtgaggg gcaacagccc cgccacgcga gcacgctcgc
     3001 gcgcctgttg tgtgcggtcg accggcggcg gctcgggcgt cggcggcggt tttacttccg
     3061 gcttcggttc gggctcgaca cggcgctcct cgacgacggg cgtgggctcg acgg
//
LOCUS       DASSNN010000580         2730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1612599, whole genome shotgun sequence.
ACCESSION   DASSNN010000580 DASSNN010000000
VERSION     DASSNN010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612599"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VFL84_10745"
     CDS             <1..1030
                     /locus_tag="VFL84_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609145.1"
                     /translation="ELGEKSATIFALEARDKALRDQLRASEDEFVVRSSAVQDAERAL
                     NDKQNELAKMMGELDEQAVIADSQKIEIVALKTQIEAMKERLSDAEKEVKATEQRVAH
                     ERVELKEATAELTAERGKVENLGRRVSELEQQLVAQTTEAEMLGRRAHDLETRLTEQS
                     RALTESEFDRKQLREQMEGARKTEADLRTALAEIGGKTNSATETLRAEKTQLQAELDR
                     ANEERTRLAREINDMKRDTEQAWSSERVENALLRERINDVAAEVARLASALEGPNSPI
                     DAILAAETARDKGALPPVNVEVEAAPEPAQSGNLGGNLGGNLADRIRALQSRASRLQP
                     SDDPASRN"
     gene            1107..1181
                     /locus_tag="VFL84_10750"
     tRNA            1107..1181
                     /locus_tag="VFL84_10750"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1139..1141,aa:Val,seq:cac)
     gene            1497..1979
                     /locus_tag="VFL84_10755"
     CDS             1497..1979
                     /locus_tag="VFL84_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET7609146.1"
                     /translation="MPATGRPSAYRLALGDKVKITVFNEPEMSGEFQVNSRGNVALPL
                     AGNVAAVNKTVDQFKHAVAAKLSGKYVRNPRIGVEVVTYQAINVIGEVKNAGQYPYRP
                     GLTMRDAVAMAGGYTYRANTHTLYVRHAGAPAEHTIDVDGVPPPIEPGDNVRVPERYF
                     "
     gene            2428..>2730
                     /locus_tag="VFL84_10760"
     CDS             2428..>2730
                     /locus_tag="VFL84_10760"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7609147.1"
                     /translation="MTATCAAGAEEAIPDDAYSRIPVGDRPHPEYDPIGYRVGSVFIY
                     PKLSGGVIYNSNVFASHRNPRADFAALISPELTIRYGKLPQVYPVEPSRFSAELNLG"
BASE COUNT          546 a          884 c          874 g          426 t
ORIGIN      
        1 cgagctcggt gagaagagcg ccaccatttt cgccctcgaa gcgcgcgaca aggcgctgcg
       61 cgaccagttg cgcgccagcg aagacgaatt cgtggtgcgc tcatccgcgg tgcaggacgc
      121 cgagcgcgcg ctcaacgaca aacagaacga gctcgccaag atgatggggg agctcgacga
      181 gcaggcggtg atcgccgaca gccagaagat cgagatcgtc gcgctcaaga cccagatcga
      241 ggcgatgaaa gaacggctca gcgatgccga gaaagaggtc aaggccaccg agcagcgcgt
      301 cgcgcatgag cgcgtcgagc tgaaggaagc gaccgcggag ctcacggccg aacgcggcaa
      361 ggtcgagaac ctcggccgtc gcgtcagtga gctcgagcag cagctcgtgg cacagaccac
      421 cgaggccgag atgctcggcc ggcgcgccca cgacctcgag acgcggctga ccgagcagtc
      481 ccgcgcgctg accgaaagcg agttcgaccg caagcagttg cgcgagcaga tggagggcgc
      541 gcgcaagacc gaagccgatc tgcgcacagc gctcgccgag atcggcggca agaccaactc
      601 tgccaccgag acgctgcgag ccgagaagac gcagctccag gccgagctcg accgtgcgaa
      661 tgaggagcgc acgcggctcg cccgcgaaat caacgacatg aagcgtgaca ccgaacaggc
      721 gtggtcgtcg gagcgggtgg agaatgcact gctgcgcgag cgcatcaacg acgtcgcagc
      781 cgaggttgcg cggctcgcct ccgcgctcga aggcccgaac tcgccgatcg acgcgatcct
      841 cgccgccgag actgcgcgcg acaagggcgc gctgccgccg gtcaacgtcg aagtcgaggc
      901 ggcaccggag ccggcgcaaa gtggcaatct tggtggcaat cttggcggca atctcgccga
      961 ccgcatccgc gcgctgcaga gccgcgcctc gcggctgcag ccttcggacg acccggcgag
     1021 ccggaattag cggcgcggcg cccggcaggg cgcgttgctt gacactccgc gggccgattt
     1081 cttatatccg cccgattgtg acgaccgggc ggttagctca gcgggagagc acacccttca
     1141 cacgggtggg gtcgtaggtt caatccctac accgcccacc attcgtcgcg cgataacgcg
     1201 gcgttatgct tcatccagaa ttcgcgcacg ggcgctcacg tgcccgaaat ccgccgcgcc
     1261 gtttccatgt tttgaccgcc aagtcttcgc gcggcagccc aacgaacacc aaaattcctc
     1321 agcctaatcc cggccgaaat cgcaagcacg catccgcgtg actttgcgat gccattgttc
     1381 gcgtcaggac accaatgcgc tggggacgac ggttgagtgc aattgcggca ttgtgtgtcc
     1441 tggtcgcagg atgcgatgga ccgagaggca ttccggcgac ggaaagcttc gcggccgtgc
     1501 cggcaaccgg caggccgtcc gcctatcggc tcgcgctcgg cgacaaggtc aagatcacgg
     1561 tgttcaacga gcccgagatg tcgggcgaat ttcaggtcaa tagccgcggc aatgtcgcgc
     1621 tgccgctcgc cggcaatgtg gcggcggtca acaaaaccgt cgaccagttc aagcacgcgg
     1681 tcgctgccaa gctcagcggc aaatacgtca ggaacccgcg catcggcgtc gaggtggtca
     1741 cctatcaggc gatcaatgtg atcggcgagg tcaagaacgc cgggcagtat ccgtatcgcc
     1801 ctggcctcac gatgcgcgac gcggtagcga tggccggtgg ttacacctac cgcgccaaca
     1861 cccacacact ttatgtccgg cacgccggcg cgcccgccga gcacaccatc gatgtcgacg
     1921 gcgtcccgcc gccgatcgag cccggggaca atgtccgcgt tcccgaacgc tatttctgac
     1981 tccacttgca cggaagaagc cacgaaagga gagcctcgat gtcgaaagcg attgcggtgt
     2041 cacgtcgcgt tccacataca gctcgctcac gcgtgacgag cgcgacgcgc ccaatccgcg
     2101 gcaaggagtt cagtccgccg ggctgaccat ctcccgccgt tcctgatcga ctgcagacct
     2161 gtcgtcacgc gaagcgcacg cttcgtgagc ggcgatgtgc ggccggcgaa ctcccgccaa
     2221 atcttttttc cgcaatgcgg aacgcaaatt gactcacgtc attgccggtc agggaaggaa
     2281 gcggctcgcg cgttgtcggt cgagcgaatc gaaccgatcc ggcgatcaac ccagaaaggg
     2341 cgatgcagcc aacgacaatg cgtcgttggc cctggttccg agtgttggag tctctgattg
     2401 agaattgggt ggatcgtggt ggcatgcatg acagcgacgt gcgcggcagg cgccgaggag
     2461 gccataccgg atgacgctta ctcgcggatt ccggtcggcg atcggccgca tccggagtac
     2521 gatccgatcg gttatcgcgt cggcagcgtc ttcatctatc cgaagcttag cggcggcgtg
     2581 atctacaatt cgaatgtctt cgccagccac cgtaatccgc gcgccgactt cgcggcgctg
     2641 atctcgcccg aactgacgat ccgttacggc aagctgcctc aggtctatcc cgtcgagcct
     2701 tccaggtttt cggccgagtt gaaccttggc
//
LOCUS       DASSNN010000581         3890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1700988, whole genome shotgun sequence.
ACCESSION   DASSNN010000581 DASSNN010000000
VERSION     DASSNN010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700988"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VFL84_10765"
     CDS             <1..380
                     /locus_tag="VFL84_10765"
                     /inference="COORDINATES: protein motif:HMM:NF028447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HET7609148.1"
                     /translation="MPLFVRAGSIVPTGPAIQHTGADKGGPVTLNVYTGADGSFSLYE
                     DDGTSRQYLHGQYSRVPLKWDEASRTLTIGAREGNGYDGMPSKRTINVRWMKPGTTRA
                     LSFDAKPDASVSYTGEPLTVRMR"
     gene            complement(381..1520)
                     /locus_tag="VFL84_10770"
     CDS             complement(381..1520)
                     /locus_tag="VFL84_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET7609149.1"
                     /translation="MRNLVCALALLFAVTTPPTPANAEHRPRARDAGITFGILPTGPL
                     NAITDVAGVRVGQVTIVEGTNIRTGVTAILPHGGNLYQDKVPAGFSVGNAYGKFMGST
                     QVEELGEIETPIVLTNTLNVPEAAAGVIEWTLKQPGNAEVRSVNAIVGETNDGTLNDI
                     RARRVRPADAVAAIEQAREGAVTEGAVGAGTGTIAFGWKGGIGTSSRKLPAKLGGWTV
                     GVLVQTNYGGVLQVAGVPVGQALGKYFLKDELSTASGDGSCIVVVATDAPLSDRNLER
                     LAHRGLLGIVRTGSPMTNGSGEYAVAFSTNLDVRRTPERRTQPSPIVDVPNDRISPLF
                     EAAVEAAEEAAINSMFAATPMDGNGRHIDALPVDEVVKLYKRARR"
     gene            complement(1620..3254)
                     /locus_tag="VFL84_10775"
     CDS             complement(1620..3254)
                     /locus_tag="VFL84_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET7609150.1"
                     /translation="MRVDRRGFLLSGAAAAYASPIRAQAAQPFSPSWESLASHYRMPD
                     WFRDAKFGIWAHWGPQCQPEWGDWYARLMYVRGRQPWFPAETAYEHHLEHYGHPSRTG
                     FLEIIGQWKAENWQPEYLLKRYQAAGARYVMAMGCHHDNLDLFASQHHAWNSVRVGPK
                     RDILGTWAPLVRDAGLKFGVSNHSGHAWHWYQVAYGYDAEGPMRGRRYDAYWLRKPHG
                     RGKFWDGLDPQDLYTGPYYVPPDGIASDQAMLDWHEKRDGQWLEGVPAGNVGFVEKWL
                     ARQKQMVEEYRPDIVYLDHQRVPFGEYGLEAVAHYYDQATRWHGRPDVVITAGQAGPF
                     ERRALVSNVERGTLDAIQPEPWQTATCIGNWHYSRRIYEEGSYKSARQVVQMLADVVS
                     KNGNLLLSIPVRGDGSIDDKEEAILDEIAAWTRRNGEAIFGTRPWRSFGEGPATRSPA
                     GAFGEDKAKPPTSEDVRFATKGGAVHAIFMEWPERERAIGSLGTRALPEASVEKVELL
                     GGPSLEFRRDADALRVTLPPRDGIVPAIRILGRGLV"
     gene            complement(3257..>3890)
                     /locus_tag="VFL84_10780"
     CDS             complement(3257..>3890)
                     /locus_tag="VFL84_10780"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HET7609151.1"
                     /translation="AAENVMPLLDPSLAEPRIWIGNGSRVAPHYDESDNIACVVGGKR
                     RFILFPTEQVENLYVGPIDRTIAGQPISMVDLDAPDYERFPRFREAEKHALVADLEPG
                     DAIYIPALWWHAVQATGDVNVLVNYWWTDHALDAGSPMHALGHGLLTISHLPEHKRRA
                     WRVLFDHYVFKANGEPAEHIPEPARGILGRSTPELRRMIRQFLMRALTSL"
BASE COUNT          628 a         1348 c         1262 g          652 t
ORIGIN      
        1 gcatgcctct gttcgtccgc gccggctcga tcgttccgac cggtcccgca atccagcaca
       61 ccggcgcgga caagggcggg ccggtcacgc tcaacgtcta caccggcgcc gacggcagct
      121 tctcgctcta cgaggacgac ggcaccagcc ggcaatatct gcacggccaa tattcacgcg
      181 tgccgctcaa gtgggacgag gcgagcagga cgctgaccat cggggcgcgc gaaggcaacg
      241 gctacgacgg aatgccgagc aagcgcacga tcaacgtccg ctggatgaag cccggaacga
      301 cgcgcgcact ttcgttcgat gcgaaacccg atgcgagcgt cagctacacc ggcgagccgt
      361 tgaccgtgcg gatgcgctga tcagcgccgc gcgcgcttgt agagcttcac cacctcatcg
      421 accggcagcg catcgatgtg gcggccgttt ccgtccatcg gggttgccgc gaacatggaa
      481 ttgatcgccg cctcctcggc ggcctcgacg gcggcttcga acagcgggga gatgcgatcg
      541 ttcggaacgt cgacgatcgg gcttggctgc gtccgccgtt cgggcgtccg ccgcacatcg
      601 aggttcgtcg agaaagccac cgcatattcg cccgagccgt tggtcatcgg cgagccggtg
      661 cgtacgatcc ccaacagccc gcgatgcgcg agccgctcga ggttgcggtc gctgagcggc
      721 gcgtcggtcg caacgacgac gatgcacgac ccgtcgccgc tcgccgtcga cagctcgtcc
      781 ttgaggaaat atttgccgag cgcctgaccg accggcacac cggcaacctg aagcacgccg
      841 ccgtaattgg tctgcaccag cacgccgacg gtccagccgc ccagcttcgc cgggagcttg
      901 cgcgagctgg tgccgatccc gcccttccag ccgaacgcga tggttcccgt ccccgcgccg
      961 accgcgcctt cggtcaccgc tccctcgcgc gcctgctcga tcgccgccac cgcatcggca
     1021 ggccgcaccc gccgcgcgcg aatatcgttg agcgtgccgt cgttggtttc gccgacgatc
     1081 gcgttgaccg agcgcacttc cgcgttgcca ggctgcttga gagtccactc gatcacgccg
     1141 gcagcggcct ccgggacgtt cagcgtattg gtgagcacga tcggagtctc gatctcgccg
     1201 agctcctcga cctgcgtcga gcccatgaac ttgccgtacg cattgccgac ggagaagccg
     1261 gcgggaacct tgtcctggta gaggttgccg ccgtgcggaa ggatggccgt gacgcccgtc
     1321 cgaatgttcg tgccctcgac gatcgtgacc tgcccgacgc gaacgccggc cacgtcggtg
     1381 atagcgttca gcggccccgt cggaagaatg ccgaaggtga tccccgcgtc gcgagcgcga
     1441 gggcgatgtt cggcatttgc gggggtggga ggcgtggtca cggcaaagag aagcgcaagc
     1501 gcgcaaacaa ggtttcgcat cggcgcatct ttggccaact cgtcattccc gcgaaagcgg
     1561 gaatcccgct gttctcgaac gaagccggac ccccgcttgc gcgggggtga cgataaaggt
     1621 caaaccaggc cgcgcccgag gatgcgaatg gccgggacga ttccgtcgcg cggcggcagc
     1681 gtgacccgca acgcatccgc atcgcgccgg aactcgaggg acggtccacc gagcagctca
     1741 accttctcga ccgaggcctc cggcaatgcc cgcgtgccga gcgacccgat ggcgcgctcc
     1801 cgctccggcc attccatgaa gattgcatgg accgcgccgc ctttcgtcgc gaagcgcacg
     1861 tcctcgctcg tcggcggctt ggccttgtcc tcgccgaacg cgcccgccgg cgatcgcgtg
     1921 gccgggcctt cgccgaagct gcgccacggc ctcgtgccga agatcgcctc gccgttgcgg
     1981 cgggtccacg cggcgatctc gtcgaggatc gcctcctcct tgtcgtcgat gcttccgtcg
     2041 ccgcgcaccg gaatgctgag cagcagattg ccgttcttcg acaccacgtc ggcgagcatc
     2101 tgcacgacct gcctcgcgct cttgtagctg ccctcctcgt agatccggcg cgagtaatgc
     2161 cagttgccga tgcaggtcgc ggtctgccac ggctccggct ggatggcgtc gagcgtgccg
     2221 cgctcgacat tcgagaccaa agcgcgccgc tcgaacggac ctgcctgccc cgccgtgatc
     2281 accacgtccg gcctgccgtg ccagcgcgtc gcctggtcgt agtaatgcgc gaccgcctcg
     2341 agcccgtact cgccgaacgg cacgcgctgg tgatcgagat agacgatgtc ggggcgatat
     2401 tcctcgacca tttgcttctg ccgcgcgagc catttctcga cgaacccgac attgccggcc
     2461 ggcacgcctt cgagccattg tccgtcccgc ttctcatgcc agtcgagcat cgcctggtcc
     2521 gacgcgatcc cgtccggcgg tacgtagtaa ggtcccgtgt aaaggtcctg cgggtcgagg
     2581 ccgtcccaga acttgccgcg accgtgcggc ttcctaagcc aataggcatc gtaccgccgc
     2641 ccgcgcatcg gaccttcggc gtcatagccg tacgccacct ggtaccagtg ccatgcgtgg
     2701 cccgaatggt tggaaacgcc gaacttcagc cccgcatcgc gcaccagcgg cgcccacgtc
     2761 ccgaggatgt cgcgcttcgg cccgacgcgg acgctgttcc acgcgtggtg ctggctcgcg
     2821 aacaggtcga gattgtcgtg gtggcagccc atcgccatca cgtatcgcgc gccggccgct
     2881 tgatagcgct tgagcagata ttcgggctgc cagttttccg ctttccactg gccgatgatc
     2941 tcgaggaagc cggtgcggct cgggtggcca taatgctcga ggtgatgctc gtacgcggtc
     3001 tccgccggga accacggctg ccgaccgcgc acgtacatca gccgcgcgta ccagtcgccc
     3061 cattcgggct ggcactgcgg cccccaatgc gcccagattc cgaacttcgc gtcgcgaaac
     3121 cagtccggca tgcgatagtg gctcgcgagc gattcccacg acggagagaa gggttgggcc
     3181 gcctgcgcgc ggatcggtga agcgtaggcc gccgccgcgc ccgagagcag gaatccgcgg
     3241 cggtcgacgc gcaaggtcag aggctcgtca gcgcccgcat caggaactgc ctgatcatcc
     3301 gccggagctc gggagtcgag cggccgagaa tgccgcgcgc gggctccggg atatgctctg
     3361 cgggctcgcc gttcgccttg aatacataat ggtcgaacag cacgcgccat gcccgtcttt
     3421 tgtgctcggg cagatggctg atggtgagca aaccgtggcc gagcgcatgc atcggcgatc
     3481 ccgcgtcgag cgcgtgatcg gtccaccagt aattgaccag cacgttgacg tcgccggtcg
     3541 cctgcaccgc gtgccaccag agcgcgggaa tgtagatcgc gtcgcccggc tccagatcgg
     3601 cgaccagcgc gtgcttttcg gcttcgcgaa accgcgggaa gcgctcgtag tcgggggcgt
     3661 cgaggtcgac catgctgatc ggctggccgg cgatcgtccg gtcgatcgga ccgacgtaga
     3721 ggttttcgac ctgctcggtc gggaacagga tgaaccggcg cttcccgccg acgacgcagg
     3781 cgatgttgtc cgactcgtcg taatgcggcg cgacgcggct gccgttgccg atccagatcc
     3841 gcggctccgc caatgacggg tcgagcaacg gcatgacatt ctcggcggcg
//
LOCUS       DASSNN010000582         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1847164, whole genome shotgun sequence.
ACCESSION   DASSNN010000582 DASSNN010000000
VERSION     DASSNN010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847164"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..996)
                     /locus_tag="VFL84_10785"
     CDS             complement(16..996)
                     /locus_tag="VFL84_10785"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HET7609152.1"
                     /translation="MHPNEEALPSDAVPEAASPRTDNAADDAAAAPDAMAAPAADAAQ
                     DSSDTLSPAVRRLVRQFDLDITGLHGTGPSGRIRVGDVMGLLAGRKDSGKRDAPPRAA
                     PPDSDAAPADDDAILEVLATEAEPQAGAEPAAHAVVPMTTVFDCDVSRVLAHRRKLRR
                     DNVELLTISYFLTALAAAVAKVPALAGGETPRFGVSLTTADGRIRGSVLEVPDPTERP
                     DEHVRAVDVALRANLHTPLEHSSLLVHHYGESGSLLATPTPIGAGHAASVGIGRIRRE
                     IVVRVVEGVETPRVAACCHLSLSFFPDRVPLHAANQALAAAVAILESWPD"
     gene            1108..1470
                     /locus_tag="VFL84_10790"
     CDS             1108..1470
                     /locus_tag="VFL84_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609153.1"
                     /translation="MKLATLAAVAVLAPSAAWAADFSGTWRLDNLFNGASSIITCTLV
                     QNGNTLTGNCKPEIPGIAPSELKGSVDGSSAKWGYDVVFNGNQARVDYVAELGADGKL
                     QGNVLRNGSPSPITGVKQ"
     gene            1526..>2412
                     /locus_tag="VFL84_10795"
     CDS             1526..>2412
                     /locus_tag="VFL84_10795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7609154.1"
                     /translation="MPSLLMAVSQEALTILLPLYMLEIGATPAFAALVVGLRGVGVLL
                     FDVPAGMLVARFGDKPVLVGGLSLIFSGLLLLSVTTEPLLLGFAAVALGSGHAAWMLG
                     RQSYLADVCAPAELGRAIAAMAGLQRGGALIGPFAGGALAGFAGYPIAFAAGVASAVV
                     AGAIVLAFARAVPPHENPDASLAGTFEVLRAQRGVLASAGGSALVLQLMRATRQLLIP
                     LFGEAVGLDVTTIGLVYSLATVVDIALFYPSGVLADRFGRKWSAVPSMALYTLGLALL
                     PLAAGFWSLLAISVLIGFA"
BASE COUNT          320 a          831 c          860 g          401 t
ORIGIN      
        1 aacttcactt ggaaactagt ctggccagct ctcgagaatc gcgacggccg ccgcgagcgc
       61 ttggttcgcg gcgtgcagcg gcacgcggtc ggggaagaac gacagcgaaa ggtggcagca
      121 cgcggcgacg cgcggcgttt cgacgccttc gacgacgcgg accacgatct cgcggcggat
      181 gcggccgatg ccgacgctcg ccgcgtgacc ggcgccgatc ggcgtcggcg tcgcgagcag
      241 gctgccgctc tcgccatagt ggtgtacgag caggctcgaa tgctcgagcg gcgtatgtag
      301 attcgcgcgc agcgcgacgt cgacggcgcg gacgtgctcg tcgggccgtt ccgtcgggtc
      361 ggggacctcg agcacggagc cgcgtatccg gccgtccgcg gtcgtcaacg acaccccgaa
      421 ccgcggcgtc tcgccgcccg cgagcgcggg caccttcgcg acggcggccg cgagcgcggt
      481 caagaaatag ctgatcgtga gcagctcgac gttgtcgcgc cgcagcttgc gccggtgggc
      541 cagcacgcgg ctcacgtcgc agtcgaacac ggtcgtcatg ggaaccacgg cgtgcgcagc
      601 cggttcggcg cccgcctgcg gctccgcttc cgtcgcgagg acctcgagga tggcgtcatc
      661 gtcggctggc gccgcatcgc tgtcgggcgg tgccgcgcgc ggaggcgcat cgcgcttgcc
      721 gctgtccttg cggcccgcga gcaagcccat gacgtcgccg acgcgaatgc ggcccgacgg
      781 ccccgtgccg tggaggcccg tgatgtcgag gtcgaactgc cgcacgagcc ggcgcacggc
      841 cgggctcagc gtgtcggacg aatcctgcgc cgcgtcggcg gccggcgccg ccatggcgtc
      901 cggagcggcc gccgcgtcgt cggccgcgtt gtcggttcgg ggtgaagccg cctccggcac
      961 cgcatcggac ggcaaggctt cttcattcgg atgcatggcg caagcatcgc gagaccgact
     1021 cgagatttca atctaggtat gatgtgccgc gttcgagctg tcgccatcgc gcgacaaggt
     1081 cgtctcgcaa acggcaggag gtagcagatg aagctcgcga cactcgcggc cgtcgcggtg
     1141 ctcgccccga gcgccgcatg ggccgcggac ttctccggca cgtggcgcct cgacaatctg
     1201 ttcaacggcg cgtcctcgat catcacgtgc acgctcgtcc aaaacggaaa cacattgacg
     1261 ggcaactgca agccggagat accgggcatc gcgccgtcgg agttgaaggg cagcgtcgac
     1321 ggctcgagcg cgaagtgggg ttacgacgtc gtgttcaacg gcaatcaagc gcgcgtcgac
     1381 tacgtcgccg agctcggcgc cgacggcaag ctgcagggca acgtgctgcg caacggcagc
     1441 ccgtcgccga tcacgggcgt caagcagtaa gcggctcaca ctgaagcttc cgccgccata
     1501 cgaccgtctg ctgctgccgg tttacgtgcc gtcgttgttg atggccgtga gccaggaagc
     1561 gctgacgatc ctattgccgc tctacatgct cgagatcggc gccacgcccg cgttcgcggc
     1621 gctcgtcgtc ggcctgcgcg gcgtcggcgt gctgctgttc gacgtgcctg cgggcatgct
     1681 cgtcgcgcgc ttcggcgaca agcccgtgct ggtcggcgga ttgagcctga tcttcagcgg
     1741 attgttgttg ctatccgtga cgacggagcc gctgctgctc ggcttcgcgg ccgtcgcgct
     1801 cggctccggc cacgcggcgt ggatgctcgg gcggcagtcg tatctcgccg acgtttgcgc
     1861 gccggccgag ctcggccgcg cgatcgccgc gatggccggc ttgcagcgcg gcggcgcgct
     1921 gatcggcccg ttcgcgggcg gcgcgctcgc cgggttcgcg ggctatccca tcgcgttcgc
     1981 ggccggcgtc gcgagcgccg tcgtagcggg cgcaatcgtg ctcgccttcg cgcgcgccgt
     2041 gccgccgcac gagaatcccg acgcgagcct cgccggcacg ttcgaggtgc tgcgcgcgca
     2101 gcgcggcgtg ctcgcgtcgg ccggcggcag cgcgctcgtg ctccagctca tgcgtgcaac
     2161 gcgccagctg ctgatcccgc tgttcggcga ggctgtcggg ctcgacgtca cgacgatcgg
     2221 cctcgtctat tcgctcgcta ccgtcgtcga catcgcgctg ttctatccct ccggcgtgct
     2281 cgcggaccgt tttggccgca agtggagcgc cgtgccgagc atggcgctct acacgttggg
     2341 cctcgcgctg ctgccgctcg ccgcggggtt ctggtcgctg ctcgcgattt ccgtgctgat
     2401 tggatttgcg aa
//
LOCUS       DASSNN010000583         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_100377, whole genome shotgun sequence.
ACCESSION   DASSNN010000583 DASSNN010000000
VERSION     DASSNN010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100377"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VFL84_10800"
     CDS             <1..596
                     /locus_tag="VFL84_10800"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HET7609155.1"
                     /translation="GIDRARAAAMLLLTQRGTPTLYYGDEIGMRGVDIPPEQAVDPQG
                     RRTGRNRDPTRTPMQWSGDTYAGFSAVEPWLPVGVDRGTANVSTQSQDAGSLLTLTRL
                     LLELRAREPDLQHGAQEPREAGSSVVAYCRGAGRRFLVVVNFERAPASYALRDDGAGR
                     VLLSTLLDREGERVADRVRLRADEGLLIALEPGSPRA"
     gene            complement(593..2659)
                     /locus_tag="VFL84_10805"
     CDS             complement(593..2659)
                     /locus_tag="VFL84_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET7609156.1"
                     /translation="MSAVGARVAAAVAETSGPIRTLTLHGVDDDRRAALLTREWLVTN
                     GLGGYASGTIGGAPSRRYHGLLIAALPPPYGRRLLLTDVSEALYLDGRRAAQLTATTA
                     DDAAHVPLEEFRLEAGLPAWRYDVGGVVIEKRMVMPHGQNTVHVLYELVAGAEFARLD
                     VTLAAHHRPHDAAVDTPLAERPPLTRSGAGYELTIGELPPLRLMIDATHTPLEEPPAA
                     WPERRYAVEESRGYMGRAGAWTPGVFALELKPAQPIAIVASTEPWEVITALEWRAAFA
                     AERDRRLACVAAAVPDAQKGFAAELVLAADQFIVTPVGRSAEAARVRAEGAEERSVIA
                     GYHWFTDWGRDTMISLEGLTLLTGRAAEAKGILRTFASAIRDGLLPNLFPEGESEGLY
                     HTADATLWLFHALDRYLMATSDRALLQALLPQLVEIVEHHTAGTRFGIGVDRNDGLLR
                     QGAPGFQLTWMDAKVGDWVVTPRRGKAVEINALWYNALCLLADWHRSVGRGGDGARFD
                     SAAAQVRDSFNRRFWHDPVGHLFDVVDGENGDDAACRPNQVIAISLAHPVLARRHWQR
                     VLRVVREELLTPYGLRTLSPKHPAYQPKYYGDLRSRDAAYHQGTVWPWLLGPFIDAWR
                     RAFPEDTAAPGQFLAAFEQHLDDGCVGSISEIFDAEAPYVPRGCVAQAWSVAEILRCV
                     VYANRH"
     gene            complement(2659..>2904)
                     /locus_tag="VFL84_10810"
     CDS             complement(2659..>2904)
                     /locus_tag="VFL84_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011427509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609157.1"
                     /translation="KIRQWAEARNGKPARVQGTGDGDDAGLLRIDFPEEHEDEERLEE
                     ISWDEFFEKFDEKRLAFVYQDKTAAGKPSRFNKFVSR"
BASE COUNT          422 a         1028 c         1007 g          447 t
ORIGIN      
        1 ccggcatcga ccgggcccgc gccgcggcga tgctgctgct cacgcaacgc ggcaccccga
       61 cgctctatta cggcgacgag atcgggatgc gcggcgtcga cattccgccg gagcaggctg
      121 tcgacccaca gggccgccgc accggccgca atcgcgaccc gacgcgcact ccgatgcagt
      181 ggagcggtga cacgtacgcg gggttctcgg ccgtggagcc gtggttgccc gtcggcgtcg
      241 accgcgggac cgcgaacgtc tcgacgcaga gccaggacgc cggctcgctg ctgacgctga
      301 cgcggctgct gctcgagctg cgcgcccgcg aaccggactt gcagcacggc gcgcaggagc
      361 cgcgcgaggc gggctcgagt gtagtcgcgt actgccgcgg cgccggccgg cgattcctcg
      421 tcgtcgtgaa cttcgagcgc gctccggcga gctacgcgct tcgcgacgac ggcgcgggcc
      481 gcgtgctgct atcgacgctc ctcgaccgag aaggcgagcg cgtggccgat cgagtccgac
      541 tgcgcgccga cgaggggctt ctgatcgcgc tcgagcccgg gtcgccgcgc gcctaatggc
      601 ggttcgcgta cacgacgcag cgcaatatct ccgccacgct ccatgcctgg gccacgcagc
      661 cgcgcggcac gtacggcgcc tcggcgtcga agatctcgct gatgctgccg acgcaaccgt
      721 cgtcgaggtg ctgctcgaac gccgcgagga attggccggg ggccgccgtg tcctcgggaa
      781 atgcgcggcg ccacgcgtcg atgaaaggac cgagcagcca gggccagacg gtcccttggt
      841 gatacgcggc atcgcggctg cgcaaatcgc cgtagtactt cggctgatag gcggggtgct
      901 tcgggctcag cgtgcggagc ccataaggag tcaacagctc ctcgcgaacg actcggagca
      961 cgcgttgcca atgacgccgc gccagcacgg gatgcgcgag cgagatcgcg atgacctggt
     1021 tgggccggca cgccgcgtcg tcgccgttct cgccgtcgac gacgtcgaac aagtgcccca
     1081 cggggtcgtg ccagaacctg cgattgaacg agtcgcgcac ttgcgctgcc gcgctgtcga
     1141 aacgcgcgcc gtcgccgccg cgcccgacgc tgcggtgcca gtcggcgagc aagcacagcg
     1201 cgttgtacca gagcgcattg atctcgacgg ctttgccgcg ccgcggcgtg acgacccaat
     1261 cgccgacctt cgcgtccatc cacgtgagct ggaagccggg cgctccctgg cgcagcaatc
     1321 cgtcgttgcg atcgacgcct atgccgaatc gcgtgcctgc cgtgtggtgt tcgacgatct
     1381 cgacgagctg cggcagcagc gcctggagca aagcccggtc gctcgtcgcc atgagatagc
     1441 gatcgagcgc gtggaacagc cacagcgtcg cgtccgcggt gtgatacagg ccttcgctct
     1501 cgccctccgg gaacaggttc ggcagaaggc cgtcgcgaat cgcgctcgcg aaagtccgga
     1561 ggatcccttt cgcttcggcc gcgcggcccg tcagcagagt cagcccttcg aggctgatca
     1621 tcgtgtcgcg gccccagtcc gtgaaccagt gataccccgc gatcacggaa cgctcttccg
     1681 cgccttcggc gcggacgcgc gccgcctcgg ccgatcgtcc gaccggcgtc acgatgaact
     1741 ggtcggcggc gagcacgagc tcggccgcga atcctttctg cgcgtcggga acggcggccg
     1801 cgacgcatgc aagccttcgg tcccgttcgg ccgcaaaagc ggcgcgccat tcgagcgccg
     1861 tgatgacctc ccacggctcg gtagaggcga cgatcgcgat cggctgcgcg ggcttgagct
     1921 cgagcgcgaa cacgcccggt gtccacgcgc cggctctgcc catgtagccg cgcgactctt
     1981 cgacggcgta ccgccgctcc ggccaagcgg cgggcggttc ctcgagcggc gtatgcgtcg
     2041 cgtcgatcat gagcctgagc ggcggcagct ctccgatcgt cagctcgtag ccggcgccgc
     2101 tccgtgtcag aggcgggcgc tcggcgagcg gcgtgtcgac ggcagcgtcg tgcggccggt
     2161 gatgcgcggc cagtgtgacg tcgagccgcg cgaattccgc gccggcgacg agctcgtaga
     2221 ggacgtgcac ggtgttctga ccgtgcggca tcaccatgcg tttctcgatg actacgccgc
     2281 ccacgtcgta gcgccaggcc ggtaggccgg cctcgagcct gaactcctcg agcggcacgt
     2341 gcgcggcgtc gtcggccgtc gtcgcggtga gttgagccgc gcgccgcccg tcgagataca
     2401 gcgcctcgct gacgtcggtc agcagcaggc gccggccgta cggcggcggc agcgccgcga
     2461 tcaggaggcc gtgatagcgc cgcgagggcg caccgccgat cgtgcccgag gcgtaaccgc
     2521 cgagaccgtt cgtgacgagc cactcgcgcg tcagcagcgc ggcgcgccgg tcgtcgtcga
     2581 cgccgtgcag cgtcagtgtg cgaatcggac cgctcgtttc ggccaccgcg gccgccacgc
     2641 gcgcgccgac ggcgctcatc agcggctgac gaatttgttg aagcggctcg gcttgccggc
     2701 cgccgtcttg tcttggtaca cgaaggcgag gcgcttctcg tcgaacttct cgaagaactc
     2761 gtcccagctg atctcttcga gccgttcctc gtcctcgtgc tcctcgggga agtcgatccg
     2821 cagcaggcct gcgtcatcgc catcgccggt cccttgcaca cgagcaggct tcccgttgcg
     2881 cgcttcggcc cactgccgaa tctt
//
LOCUS       DASSNN010000584         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1701351, whole genome shotgun sequence.
ACCESSION   DASSNN010000584 DASSNN010000000
VERSION     DASSNN010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701351"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFL84_10815"
     CDS             <1..651
                     /locus_tag="VFL84_10815"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET7609158.1"
                     /translation="GSTAYSTLGYFDDPVLSTMVAGGEQYVASLLFHELAHQRLYVKS
                     DSEFSEAFAMVVEELGTERWLSEHGTAADLERYRTRRRHREQFGELIAAQQARLRAIY
                     ATGAAPEQLREDKRRAFDTLRREYEALKASWQGTTDYDAWFAQPLNNASLASVATYTR
                     WLPALRARLDEVGLRAFYADAAAVAKLDVEEREEKLRAWAAKARAPRSDGDVSASG"
     gene            complement(635..2029)
                     /locus_tag="VFL84_10820"
     CDS             complement(635..2029)
                     /locus_tag="VFL84_10820"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HET7609159.1"
                     /translation="MSHGLLFTPRFLAGMRYCAALAVACAGGLALAQADAPRELPTLA
                     PMIATVAPAVVNIAVTTNIKSPGGQTQDELLRRFFDFDPPPGGRDREIEGAGSGVIVD
                     SANGYILTNHHVIANADKITVTLLENRSLTARIVGSDEASDLAVLQVDAADLTSIPFG
                     DSSSLHVGDYVVAIGNPFGFTNSVTSGIVSALGRSGLNPQAFEDFIQTDASINPGNSG
                     GALVDLNGKLVGINSAIISRTGGNVGIGFSIPVNMVRTIMQQLVATGTVRRGLLGVGI
                     RDVTPDIAATLELPSNSGALVYEVRPDTAAERAGIQIGDVIVSVNAVRLHDSGSLRSA
                     VGLLPPGEKVSVTLLRDGREQTLTAVLGELAPTRVTAVPREERDEPDDLDVIFEGAEI
                     VENTGPNGGLLVARVDPNSPAGERGLQQGDIITKVNRVRIRTMAEAVPIMEKARAILL
                     EVQRGERSSLILMR"
     gene            2519..>3710
                     /locus_tag="VFL84_10825"
     CDS             2519..>3710
                     /locus_tag="VFL84_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HET7609160.1"
                     /translation="MNMAFELKATRLADIALQEASLNIWDTKYRLKTKSGEPVDRDID
                     GTYQRVAKALAAVEEPEKRGEWYEKFLWALQRGAIPAGRITSNAGAEYHKPATSTINC
                     TVSGTIRDSMHDILDKVHEAGLTLKAGCGIGYEFSTLRPRGAYVSGAGAYTSGPLSFM
                     DIYDKMCFTVSSAGGRRGAQMGTFDVGHPDAMDFIRAKRENGRLRQFNLSLLITDEFM
                     QAVKADQPWKLAFPLTRREAEEENIDLDDDEQVVWREWPAADHYVVDDGGLVACRVYR
                     TLPARRMWDVIMSSTYDFAEPGFVLIDRVNEMNNNWWCEDIRATNPCGEQPLPPYGAC
                     LLGSVNLTTFVRDPFGPKARFDMDEYKEVVRVFTRMLDNVVEINGLPLEKQRGEIMRK
                     RRHGM"
BASE COUNT          691 a         1148 c         1234 g          637 t
ORIGIN      
        1 ggatcgacgg cgtactccac gctcggctat ttcgacgacc ccgtgctgag cacgatggtc
       61 gccggcggcg agcagtacgt cgcaagcctg ctgttccacg agctcgcgca ccagcgtctg
      121 tacgtcaaga gcgacagcga gttcagcgaa gcgttcgcca tggtggtcga agagctcggc
      181 acggagcgtt ggctgtcgga gcacgggacg gccgcggacc tcgagcgcta tcgcacgagg
      241 cgccggcatc gcgagcaatt cggcgagctg atcgcggcgc agcaagcacg gctacgcgcg
      301 atctacgcga cgggcgctgc gcccgagcaa ttgcgcgaag acaaacggcg cgctttcgac
      361 actcttcggc gcgagtacga ggcactgaaa gcgagctggc agggaactac cgactacgac
      421 gcgtggttcg ctcaaccgct caacaacgcg tcgctcgcgt cggttgccac ttacacgcgc
      481 tggttaccgg cgctgcgcgc gcggctcgac gaggtgggcc tccgcgcgtt ctatgccgac
      541 gcggcggcgg ttgcgaagct cgatgtcgag gagcgcgagg aaaagctgcg tgcctgggcc
      601 gcaaaagctc gcgcgccgcg ctccgacgga gacgtcagcg catcaggatg agcgagctgc
      661 gctcgccgcg ctgaacctcg agcaggatcg cgcgggcctt ctccatgatc ggcaccgctt
      721 cggccatcgt tcgaatccga acgcggttca ccttggtaat gatgtcgccc tgctgcaagc
      781 cgcgctcgcc ggccgggctg ttcggatcga cgcgcgcgac gagcaagccg ccgttcggac
      841 cggtgttctc gacgatctcg gcgccctcga atatcacgtc gagatcgtcg ggctcgtccc
      901 gctcttctct cggcacggcc gtcacgcgcg tcggcgcgag ctcgccgagc acggccgtca
      961 acgtctgctc gcggccgtcg cgcagcagcg tcacgctgac tttctcgccc ggcggcaaca
     1021 agccgaccgc gctacggagc gagcctgagt cgtggagccg caccgcgttg acgctgacga
     1081 tgacgtcgcc gatttgaatg ccggcgcgct cggcggccgt gtctggcctg acttcgtaga
     1141 ccaacgctcc cgagttgctg ggaagctcga gcgtggcggc aatgtcgggc gtgacgtccc
     1201 ggattccgac accgagcagc ccgcggcgca ccgttcccgt tgcgacgagc tgctgcatga
     1261 tcgtgcggac catgttgacg ggaatcgaga agccgatgcc gacgttgccg ccggtgcgcg
     1321 aaatgatcgc ggaattgatg ccgacgagct tgccgttcaa gtcgacgagc gcgccgccgg
     1381 aattgccggg attgatcgac gcgtcggtct ggatgaagtc ctcgaacgct tgaggattga
     1441 gaccgctgcg gccaagcgcg ctgacgatgc cggacgtgac gctgttcgtg aagccgaacg
     1501 gattgccgat cgcgacgacg taatcgccga cgtgcagaga cgaggagtcg ccgaacggga
     1561 tgctggtcag atccgcggcg tcgacttgca gcactgccag atcggaagcc tcgtcggagc
     1621 cgacgatgcg tgccgtgagg ctgcggttct cgagcaacgt gaccgtgatt ttgtcggcgt
     1681 tcgcgatgac gtggtgattc gtcaggatgt agccgttcgc cgagtcgacg atgactcccg
     1741 agcccgcgcc ctcgatctcg cggtcgcgac cgcccggcgg aggatcgaag tcgaagaaac
     1801 gccgcagcag ctcgtcttgc gtttgcccgc cggggctctt gatgttcgtc gtcacggcga
     1861 tattgacgac cgcgggcgcg accgtcgcga tcatcggcgc cagggtcggc aactcgcgcg
     1921 gcgcatcggc ttgcgcgagc gcaagcccgc cggcgcacgc aacggcgagc gccgcgcaat
     1981 agcgcatgcc agcgagaaat ctcggggtga acagcaagcc gtgactcatc tgaacgatac
     2041 ctattgggtc ggagcatgcg cggccagcgc agcatcctaa gcatagcctg gcgaccggtc
     2101 gttctcgaag cgaccgccgc tgtgaattac ctgtgcggag gcgggggata ggctgggact
     2161 agcctgcata caatatattg tggtcaaacc gcgtttgccc gagggtcgcg aggtcgcgaa
     2221 aaaaatttga tccgattcgg agatgttcgg aataacccat tgttctatat tgtttcttgg
     2281 ccgagctaga aattgtcata aaaccttgac agctattctg tgcaggccta ctctacgttc
     2341 caacgacaca agatcttgtg tcgcgcacgt gaaaagaaat tcttgattgg atcaaggctg
     2401 cgcaagcagc cgacggggag tttttcgcgc cgaaaaactg cggggaaccc gtccggtcca
     2461 acgagatgcg tgaaccgggg tggggttacg gcgtagcttg gggggatcaa gagcacctat
     2521 gaacatggcg tttgagctca aagccacgag actcgcggac attgctctgc aggaagcctc
     2581 attaaatatc tgggatacga agtaccgcct gaagacgaaa tcgggcgagc ccgtcgatcg
     2641 cgacatcgac ggcacttacc agcgcgtcgc gaaagcgctc gcggcggtcg aggagcccga
     2701 gaagcgcggc gagtggtacg agaagtttct ctgggcgctg cagcgcggtg cgatcccggc
     2761 cggccgaatc acgtcgaatg ccggcgccga gtaccacaag cccgcgacgt cgacgatcaa
     2821 ctgcacggtg tcgggcacga tccgcgactc gatgcacgac atcctcgaca aggtgcacga
     2881 ggccggcctc acgctgaaag cgggttgcgg catcggctac gagttctcga cgttgcgtcc
     2941 gcgcggcgcc tatgtcagcg gcgcgggggc ctacacgtcc ggcccgctct cgttcatgga
     3001 tatctacgac aagatgtgct tcaccgtgtc gtccgccggc ggccggcgcg gcgcgcagat
     3061 gggcacgttc gacgtcgggc accccgacgc gatggatttc attcgtgcca agcgcgagaa
     3121 cggccggctg cgccagttca acctgtcgct gctgatcacg gatgagttca tgcaagcggt
     3181 gaaggccgat cagccttgga agctcgcgtt tccgctgacg cggcgcgagg cggaggaaga
     3241 gaacatcgat ctcgacgacg acgagcaagt cgtgtggcgc gagtggccgg ccgccgacca
     3301 ctatgtcgtc gacgacggcg ggctcgttgc ctgccgcgtc tatcggacgc tgcccgcacg
     3361 gcgaatgtgg gacgtcatca tgtcgtcgac gtacgacttc gccgagcccg ggttcgtgct
     3421 catcgatcgc gtcaacgaga tgaacaacaa ctggtggtgc gaggacattc gcgccacgaa
     3481 tccgtgcggc gagcaaccgc tgcccccgta cggcgcttgc ctgctcggct cggtcaacct
     3541 caccaccttc gtgcgcgatc cgttcgggcc gaaggcgcgc ttcgacatgg acgaatacaa
     3601 ggaagtagtg cgcgtgttca cgcgcatgct ggacaacgtg gtcgagatca acggcctgcc
     3661 gctggaaaaa cagcgcggcg agatcatgcg caaacgccgc cacggcatgg
//
LOCUS       DASSNN010000585         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1484586, whole genome shotgun sequence.
ACCESSION   DASSNN010000585 DASSNN010000000
VERSION     DASSNN010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484586"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFL84_10830"
     CDS             complement(<1..962)
                     /locus_tag="VFL84_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HET7609161.1"
                     /translation="MEPVLKRGYSSWDRDVLPEDEYALRVEAVRAKLREQGLAALVVV
                     NYSLLGVMVDYADMAYLSGLQSGGALLLPLDGAPVYVGFGGGRELSFMRTLTWLDHVI
                     SGCGPKAFEVVREQLRAQGISNGPIAIAGAADLAAGAAARVQQAFEGYTLKPFDAELR
                     ALRAVKRPREILAMRLTLGVVEDAVHAGLAKFVGGGDNAAAMLEAERVARARKARDVR
                     VLVNMGGPELRPFEGRLDGRHSPVRLWVAAQYQGYWAEAAAMAPVSQPTAAQKAVAAM
                     LAAAGAGATTGEVAVAAIAALSPAEAQAALEYGLGGTIGLSHDEG"
     gene            complement(984..>2039)
                     /locus_tag="VFL84_10835"
     CDS             complement(984..>2039)
                     /locus_tag="VFL84_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET7609162.1"
                     /translation="VAYGSGHHAFTGTNPAWYVAAYKQLGPHMAVILPREGEPLLVLT
                     PTWDAARYRERATMDFVAVQSEDFLAHVGAEIKKRGLRGKRLAVAGGNLQPKAIAEAW
                     PHELGSAPLNAEKLISDMAKIRDEWSLSCTRRGVAIAERGYQNLLETTTVGMREYELA
                     ANLEVFMRSLGGEDNFQLMSASQHNTAGHFPTDRVLQKGDLVLAEITPAVEGEFIQIC
                     RSAVLGGPSPLQQEKFALLTQALEAGMRAAKPGVSVTEIVRVINEPIAAAGYAEYTKP
                     PYMRTRGHSMGLGSMEPEVAPGYEHYLQEDMVFVMHPNQYIPETGYIMCGEPVIVTKD
                     GAKNLTSRMGTLGTIEA"
BASE COUNT          301 a          742 c          670 g          326 t
ORIGIN      
        1 ccttcgtcgt gcgagagccc gatcgtgccg ccgagcccgt attcgagcgc ggcctgcgct
       61 tcggcgggcg agagcgcggc gattgcagcc accgcgactt cgcccgtcgt cgcgcccgcg
      121 cccgccgcgg ccagcatcgc cgcgacggcc ttctgcgccg cggtcggctg cgagaccggc
      181 gccatcgcgg cggcctcggc ccaataacct tggtactgcg ccgcgaccca gagccgcacc
      241 ggcgaatgcc ggccgtcgag ccgcccttcg aacggccgca gctcgggacc gcccatgttg
      301 acgagcacac gcacgtcgcg cgccttgcgc gcacgcgcga cgcgctcggc ctcgagcatg
      361 gccgcggcgt tgtcgccgcc gccgacgaat ttcgcgagac cggcgtgcac cgcgtcctcg
      421 acgacgccga gcgtcaagcg catggccaga atctcgcgcg gccgcttgac ggcgcgcagc
      481 gcgcgcagct ccgcgtcgaa cggcttcagc gtgtaaccct cgaacgcctg ctgaacgcgc
      541 gcggcggcgc cggctgcaag atccgcggcg cccgcgatcg cgataggccc attcgagatt
      601 ccttgcgcgc gcaactgctc gcgcacgacc tcgaacgcct tcggcccgca gcccgagatc
      661 acgtggtcga gccaggtcag cgtgcgcatg aaactaagct cgcggccgcc gccgaacccg
      721 acgtagacgg gtgcgccgtc gagtgggagc agcagcgcgc cgcccgattg aagccccgac
      781 aaatacgcca tgtcggcgta atcgaccatg acgccgagca ggctgtagtt gacgacgacg
      841 agcgccgcga ggccttgctc gcgcaacttc gcgcgcacgg cctcgacgcg cagcgcgtac
      901 tcgtcctccg gcaacacgtc gcgatcccac gacgagtagc cgcgcttcag gaccggttcc
      961 atgctgatca acgctgcaag ctcctacgct tcgatcgtcc cgagcgtgcc catgcggctc
     1021 gtcaaattct ttgcgccgtc tttcgtgacg atgacgggct cgccgcacat gatgtagccc
     1081 gtctccggga tgtactgatt cgggtgcatg acgaacacca tgtcttcctg caggtagtgc
     1141 tcgtatcccg gagcgacctc gggctccatc gagcccaagc ccatgctgtg gccgcgcgtg
     1201 cgcatgtacg gcggcttcgt gtactcagcg tagcccgcgg ccgcgatcgg ctcgttgatg
     1261 acgcggacga tctcggtgac ggagacgccg ggcttcgcgg cgcgcatgcc ggcttcgagc
     1321 gcttgcgtca gcagcgcgaa cttctcctgc tgcagcggcg acgggccgcc gagcacggcg
     1381 ctgcggcaga tctggatgaa ctcgccttcg acggccggcg tgatctcggc gagcacgagg
     1441 tcgcccttct gcagcacgcg gtcggtcggg aagtggcccg ccgtgttgtg ctgcgacgcg
     1501 gacatgagct ggaagttgtc ctcgccgccc aagctgcgca tgaacacctc gaggttcgcc
     1561 gcgagctcgt actcgcgcat gccgaccgtc gtcgtctcga gcaagttctg gtagccgcgc
     1621 tccgcgatcg cgacaccgcg gcgcgtgcag ctcagcgacc attcgtcgcg gatcttcgcc
     1681 atgtcgctga tcagcttctc cgcgttcagc ggcgcactcc caagctcgtg cggccacgct
     1741 tctgcgattg ctttcggctg caagttgccg cccgcgacgg cgagccgttt gccgcgaaga
     1801 ccccgcttct tgatctcggc gccgacatgc gcgagaaaat cctcggactg cacggcgacg
     1861 aagtccatcg tcgcgcgctc gcggtagcgt gccgcgtccc aggtcggcgt cagcaccagc
     1921 agcggctcgc cctcgcgcgg caggatcacg gccatgtgcg ggccgagctg cttgtacgcg
     1981 gccacgtacc acgcggggtt cgtgcccgtg aacgcgtggt ggcccgagcc gtacgcgac
//
LOCUS       DASSNN010000586         4510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1614003, whole genome shotgun sequence.
ACCESSION   DASSNN010000586 DASSNN010000000
VERSION     DASSNN010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614003"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VFL84_10840"
     CDS             complement(<1..1202)
                     /locus_tag="VFL84_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HET7609163.1"
                     /translation="MSYPTPPTYTVNTAITPLDPTVTGSVRTYAIAPDLPPGLSLDAT
                     TGRISGTPTTPWRARHTVVATNSGGNTTFALTIAVVTERSTTDRTPDEASGLQVHVMY
                     VIPSDGVDEQLDQTATLDQSLKIANEWFKSQTGGKGIRFDTYGGGKLDVTFLQLTKSD
                     VEMNVSGGNVRTKLEYQLFAKGFDSPDKVYLVYYGGNGDGCGRGAWPPTLHGNVAAVY
                     IGAASNCVNQPFAVATQPPGFLEFMGLHEILHVLGFAAPCAPNLTEGGHVGDSVQDLM
                     YSGSQPWQPSTVDVNHDDYFGPMGTGCLDLANSAFLDPLPAGAEPPPKWPYATLTDLG
                     CGGSATPGTAGAPTQIMFVNSLSSAVQVYERVPSTTTPGTYQNSYRMDVPANDGAVLA
                     VYQALPVT"
     gene            complement(1530..3785)
                     /locus_tag="VFL84_10845"
     CDS             complement(1530..3785)
                     /locus_tag="VFL84_10845"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HET7609164.1"
                     /translation="MMASSAATKRIERIGYALLGAAVALLGACANPSYQSGAASTGSA
                     QPGGQGLDEITVTGSRVYGPSGSPRRVEPRDRQATLAVAPANAEAGGESIAGMRPGGS
                     TLLSQVQPGEEIWVIQTSSAATETEDDEPGSGTMLALVAPARGAPATEVPLPLRHTDV
                     HAVITGYISAVDVTQQFENPYDEKIEAVYLFPLPEKAAVNEFVMTIGQRKIRGILRER
                     EEAQRIYEDARAQGYRASLLVQHRPNIFEQKVANIEPGKRIDVNIRYFHTLAYEDGWY
                     SFVFPTVVGPPYNPLRSPSPVAALPREEFRDPPIGTAVRYLRPSERSAHDLGIAVDVD
                     AGVQIEELAASHEIATSRDGESRAHVTLAAGDTIPNRDFILKFRVAGETIKSNLLTYT
                     DERTQQGYFTLLVYPPRTHNDLKRQPLEMVFVVDTSGSMAGAPIGQARDAINAALVRL
                     DPRDTFQIMNFSNDVRQFASKPLPATYDNIERARDYVRRLGGDGGTEMLGGIRAALGF
                     PRDPERTRYVTFLTDGYIGNEAQILGEVQRTIGDARIFSFGVGNSVNRYLLDGLATEG
                     RGTVAYLGLEDSAADVMDYYFERISRPALTDVAIDWDGLAATDVYPTRLPDLFPGRPI
                     VVTGKFRGTPDGVAVRGRLGAGTVSLDVAGARDSRQPALRSIWARLRIEELARRQTLT
                     GDPNGALADSIRATALEHSLMSAYTSFVAVDATERTAGERGTTVNQAVPVPAGVRYDT
                     TVSGERRTIVE"
     gene            complement(3788..4249)
                     /locus_tag="VFL84_10850"
     CDS             complement(3788..4249)
                     /locus_tag="VFL84_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609165.1"
                     /translation="MSRRARALHTALAVLCAATVLSTAAQPEASYFTSVDLFVVSDAP
                     VAAWQVELTERRGAMQIVGIERGDDATFRDPPFYDRVAMTRAATDRIVLASFSLSDAA
                     QLQSGRVRIARVHVRMTGTQEPDYEARLVAAGTADGRPIDAQLRIETQTER"
     gene            complement(4246..>4510)
                     /locus_tag="VFL84_10855"
     CDS             complement(4246..>4510)
                     /locus_tag="VFL84_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609166.1"
                     /translation="SRDRRSRWAVPLGAAAAVLLAAVLMRPLVFAPPAADDVDGSGRV
                     DVLDVLALARMRAAGGDASGITDARIEELAYRIVALDSARRVP"
BASE COUNT          683 a         1533 c         1548 g          746 t
ORIGIN      
        1 tctgtaactg ggagtgcctg gtacacagcc aagacggcgc cgtcattcgc ggggacgtcc
       61 attcggtagc tgttctgata cgtgccggga gtggtcgtgc tcggcacgcg ctcgtaaacc
      121 tgaaccgccg acgacaaact gttcacgaac attatttgag tcggcgcacc tgcggtcccg
      181 ggggtcgccg atcccccgca acccaagtcg gtgagcgtgg cgtacggcca cttcggcggc
      241 ggctccgccc cggccggcaa cggatcgagg aacgcgctgt tcgcgaggtc taggcagccg
      301 gtacccatcg gtccgaagta gtcgtcgtgg ttgacgtcga cggtcgacgg ctgccacggc
      361 tgtgagccgg aatacatgag gtcttggacg ctgtcgccga cgtgcccgcc ttcggtcaag
      421 ttcggcgcgc acggcgcagc gaagccgagc acgtgcagga tttcgtgcaa acccatgaac
      481 tcgaggaagc ccggcggctg cgtggcgacc gcgaagggct ggttcacgca gttggaagcg
      541 gcgccgatat agaccgcggc gacgttgccg tgcagcgtcg gcggccacgc gccgcggccg
      601 cagccgtcgc cgttgccgcc gtagtaaacg agatagacct tgtcggggga atcgaacccc
      661 ttggcaaaca attggtactc gagctttgtg cgcacgttgc cgcccgacac gttcatttcg
      721 acatcgcttt tcgtcagctg caagaacgtg acgtcgagct tgccgccgcc gtacgtgtcg
      781 aatcgaatgc ctttgccacc ggtctgcgac ttgaaccact cgttcgcgat cttcagcgac
      841 tgatcgagtg tggcggtttg atcgagttgc tcgtcgacgc cgtcggacgg aatcacgtac
      901 atgacgtgca cctgaaggcc gcttgcctcg tcgggggtgc ggtccgtcgt cgagcgctcg
      961 gtcaccacgg caatcgtcaa cgcgaacgtc gtgttgccgc cggagttcgt cgccacgacc
     1021 gtgtgcctgg cgcgccacgg cgtcgtcggc gttcccgaga tcctgcccgt cgtcgcgtcg
     1081 agcgagaggc cgggaggcaa gtccggcgcg atcgcgtaag tccgcaccga gcccgtgacg
     1141 gtggggtcga gcggcgtgat cgccgtgttg acggtgtagg tcggcggggt tggatagctc
     1201 aatcccgacg gcggttgcgt cgcgggaggt ggcggcggcc caccggagcc gccacctcca
     1261 ccgcccccac cgctgctccc tccaccaccg catgcggtca gtccaagcac aacgaccacc
     1321 aacggcagcc atccggctgc gcttcgcaac gagagcttca tgggggttcc tccctgaggc
     1381 ggcgctcccc agcgccgcac gtacgcccac gaagcaagtt gcgtgccacg cgcgcaaaac
     1441 ggcgcatcag cggcgcgaac cgcgcgcacg cgatcaatgc gtgcgcgagt tttatatttt
     1501 ttggaagccg agcgagacgc ttgcgtccat cactccacga tcgttctgcg ctcgccgctc
     1561 acggtcgtgt cgtaacgcac acccgcggga acgggcaccg cttggttcac cgttgtgccg
     1621 cgctcgccgg ccgtgcgctc cgttgcgtcg acggcgacga acgacgtgta cgcagacatc
     1681 aagctgtgct cgagcgccgt cgcccgtatc gaatcggcga gcgcgccgtt cgggtctccg
     1741 gtcaacgtct gccgtctcgc gagctcctcg atgcggagcc gtgcccagat gctgcgcagc
     1801 gccggctgac gcgagtcgcg cgcgcctgcg acgtcgagcg agacggtgcc cgcgccgagc
     1861 cgcccgcgaa cggcgacgcc gtccggcgtg ccgcggaact tgcccgtcac gacgattggg
     1921 cgccccggaa ataagtccgg caaacgcgtg ggatagacgt ccgtagcggc gagcccgtcc
     1981 caatcgatcg cgacgtcggt cagcgcgggc ctgctgatgc gctcgaagta gtagtccatg
     2041 acgtcggccg ccgagtcttc caagccgaga tacgcgacgg tgccgcggcc ttccgtcgca
     2101 aggccgtcga gcaggtagcg attcacggag ttgccgaccc cgaagctgaa gatgcgcgca
     2161 tcgccgatcg tgcgctgcac ttcgcccagg atctgcgctt cgttgccgat gtagccgtcg
     2221 gtcaagaacg tcacgtagcg cgtgcgctcg ggatcgcgcg ggaaacccaa ggccgcgcga
     2281 atgccgccga gcatctccgt gccgccgtct cctccgagcc ggcgcacgta atcgcgcgcg
     2341 cgctcgatgt tgtcgtaggt ggccggcagc ggcttgctcg cgaactgccg cacgtcgttc
     2401 gagaaattca tgatctggaa cgtgtcgcgt gggtcgaggc gaacgagcgc cgcgttgatc
     2461 gcatcgcgcg cctgcccgat cggcgcgccg gccatcgagc cggacgtatc cacgacgaac
     2521 accatctcga gcggctgccg cttcaggtcg ttgtgcgttc gcggcggata gacgagcagc
     2581 gtgaaatagc cttgctgcgt ccgctcgtcc gtataggtca gcaggttcga cttgatcgtc
     2641 tcgcccgcga cgcggaattt caggatgaag tcgcggttcg gaatcgtgtc gcccgccgcg
     2701 agcgtcacgt gcgcgcgcga ctcgccgtcg cggctcgtcg cgatctcgtg gctcgcggcc
     2761 agctcctcga tctggacgcc ggcgtcgacg tccacggcga tgccgaggtc gtgcgcggag
     2821 cgctcgctcg ggcgcagata ccgcacggcc gtgccaatgg gcgggtcgcg gaactcctcg
     2881 cgcggcagcg cggcgacggg gctcggcgag cggagcgggt tgtagggcgg gcccaccacg
     2941 gtcgggaaca cgaacgagta ccagccgtcc tcgtaggcca gcgtgtggaa atagcggatg
     3001 ttgacgtcga tgcgcttgcc cggctcgatg ttcgcgactt tctgctcgaa gatgttcggc
     3061 cgatgctgca cgagcaagct cgcgcggtag ccctgggcgc gcgcgtcctc gtagatgcgc
     3121 tgcgcctctt ccctctcgcg caggatgccg cggatcttcc gctggccgat cgtcatcacg
     3181 aactcgttga cggcggcctt ctccggcagc ggaaacagat agacggcctc gatcttctcg
     3241 tcgtatgggt tctcgaattg ctgcgtcacg tcgacggcgg agatgtagcc cgtgatgacg
     3301 gcgtgaacgt ccgtgtggcg caacggcagc ggcacctcgg tcgccggcgc gccgcgcgcg
     3361 ggcgccacga gcgcgagcat cgtgccggag ccgggctcgt cgtcctcggt ctccgtggcc
     3421 gccgacgacg tctggatgac ccagatctcc tcgccgggct gcacctgcga gagcagcgtc
     3481 gagccgccgg ggcgcatgcc cgcgatcgat tcgccgccgg cctcggcgtt cgccggggct
     3541 acggcgagcg tcgcttgccg gtcacgcggc tccacgcgcc gcgggctgcc gctcgggccg
     3601 tagacgcgcg atccggtaac cgtgatctcg tcgagacctt gcccgcccgg ctgcgccgac
     3661 ccggtcgacg ccgctcccga ctgatacgac gggttcgcgc atgcgccgag caaggcgacc
     3721 gccgcgccga gcaatgcgta gccgatgcgt tcgatgcgtt tcgtcgcagc gctcgaggcc
     3781 atcatggtca tctctccgtt tgcgtttcga ttctgagctg cgcgtcgatc gggcgaccgt
     3841 cggccgtgcc ggcggcgacg agacgcgctt cgtaatcggg ctcttgtgtg ccggtcatgc
     3901 gcacgtgcac gcgcgcgatc cgcacgcggc cgctctgcaa ttgcgccgca tcgctcaagc
     3961 tgaagctcgc gagcacgatg cgatcggtcg cggctcgcgt catggcgacg cggtcataga
     4021 acggcgggtc gcggaacgtc gcgtcgtcgc cgcgctcgat gccgacgatc tgcatcgcgc
     4081 cgcgccgctc cgtcagctcg acctgccacg cggcgacggg cgcgtcgctg acgacgaaca
     4141 agtcgacgct cgtgaagtac gaagcttcgg gttgcgccgc cgtcgacaac acggtcgccg
     4201 cgcagagcac tgcgagcgcg gtgtgcagcg cgcgggcccg gcggctcacg gcactctccg
     4261 cgcggagtcg agcgcgacga tgcggtacgc gagctcctcg atgcgcgcgt cggtgatgcc
     4321 cgacgcgtcg ccgccggcgg cgcgcatgcg cgcgagcgcg agcacgtcga gcacgtcgac
     4381 tcttcccgag ccgtcgacgt cgtcggcggc gggcggcgcg aaaacgagcg gccgcatgag
     4441 gacggccgcc aacagcacgg cggccgccgc gccgagcgga accgcccaac gcgagcgccg
     4501 atcgcgcgaa
//
LOCUS       DASSNN010000587         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_964034, whole genome shotgun sequence.
ACCESSION   DASSNN010000587 DASSNN010000000
VERSION     DASSNN010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964034"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>2322
                     /locus_tag="VFL84_10860"
     CDS             18..>2322
                     /locus_tag="VFL84_10860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7609167.1"
                     /translation="MSLYQDPAGLVWVGTRAGGVSRWNPRSLQFGGHRPAWLENQPIT
                     AFADAPDNQLWIASLAGLVRFDTAAATVTTIDALVGRKNAIGDRPVTSLRHDRRGALW
                     IGTMGGGLKVLMPDGRIESIPVEPGDPRSLGTDGMLALLESRSGAIWIGTFGGGVNVL
                     DPATRTVRQLPYGSDKPGAVSAPVVTAIVEDARGNVWIGTEGGGLDLARADGTVLKVF
                     RNDPADASSLPSNTVYALAVDEDGRLWAATEAGGLARVVDPEAEPDAIRFQVLSRADG
                     LSSDTLYGIVPDARGRLWVSGNAGLMRLDPATGAVKTYHREHGLQGEEFSFGAYFRLR
                     DGRVAFGGAGGFNVFDPAAVSENAEPPRIALTNVDVLGVRAGGDTPFWLRDTIALDYR
                     GSIVSLDFGVLDFNSPEHNRLAYRMSGLTDDWIDLGAQRRVTLTNLDYGDQVLEVRAA
                     NSDSVWSETPLRLTIRRDPAPWVSPWAYALYALVVAAFVGQRIRRHRQKIREMVEARA
                     RLEAEVQLRTRELTESNRQLAEAARAKSDFLDRMSHELRTPMNGVVGMTELLARTSLS
                     ATQSHLTKTIRSSAQILLQIVNDLLDLSKIRAGKVALEKLPVDLKQVLEECTSIFAGA
                     ADSKGIELIVCPPPPQRRVLLGDPLRLRQVLMNLVGNAVKFTERGEVVVRADVEAAEG
                     DRAIVRLAVTDTGIGMDAAAMDKIFEPFAQADETTTRRFGGTGLGLAICRELADLMNG
                     EIAVESQPGVGSTFRLSLPAALGAEIVP"
BASE COUNT          337 a          796 c          826 g          363 t
ORIGIN      
        1 tgcgcgattc gttcgtcatg tcgctgtatc aagaccccgc cgggctcgtc tgggtcggca
       61 cgcgcgcggg cggcgtcagc cgctggaacc cgcgcagctt gcagttcggc gggcatcggc
      121 cggcctggct cgagaatcag ccgatcacgg ctttcgccga cgcgccggac aaccagctct
      181 ggatcgcgtc gctggcgggg ctcgtgcgtt tcgataccgc cgccgcgacc gtgacgacga
      241 tcgatgcgct cgtcggccgc aagaacgcga tcggcgatcg ccccgtcacg tcgctgcggc
      301 acgatcgccg tggtgcgctc tggatcggca cgatgggcgg cgggctcaaa gtgctgatgc
      361 ccgacggacg aatcgagtcg attccggtcg agcccggcga tccgcgttcg ctcggcaccg
      421 acggcatgct ggcccttctc gagtcgcgca gcggcgcgat ctggatcggc actttcggcg
      481 gcggcgtgaa cgtgctggat ccggccacgc ggaccgttcg tcagctcccg tatggctcgg
      541 acaagccggg cgcggtcagc gcgccggtcg tgacggcgat cgtcgaggac gcgcgcggca
      601 acgtctggat cggcacggaa ggcggcgggc tcgatctcgc gcgcgccgac ggcacggtgc
      661 tgaaagtctt ccgcaacgat ccagccgacg cgtcgtcgct gccgtcgaac acggtttacg
      721 cgctcgccgt cgacgaggac gggcgcctct gggcggcgac cgaggcgggc gggctcgcgc
      781 gagtcgtcga ccccgaggcg gagcccgacg cgataaggtt tcaggtgttg tcgcgcgccg
      841 acggcctgtc gagcgacacg ctgtacggca tcgtgcccga tgcgcgcggc aggttatggg
      901 tcagcggcaa cgccgggctc atgcggctcg atcccgcgac gggcgccgtc aagacctatc
      961 accgcgagca cggcctgcag ggcgaggagt tctcgttcgg cgcgtacttt cggttgcgcg
     1021 acgggcgcgt cgcgttcggc ggggcgggcg gcttcaacgt cttcgacccc gccgcggtgt
     1081 cggagaacgc ggagccgccg cgcatcgcgc tgaccaatgt cgacgtcctc ggcgtgcgcg
     1141 ccggcggcga cactccgttc tggctgcgcg acaccatcgc gctcgactat cgcgggtcga
     1201 tcgtgtcgct cgatttcggc gtgctcgatt tcaattcgcc ggagcacaat cggctcgcgt
     1261 accgcatgag cggcctcacg gacgattgga tcgacctggg cgcgcagcgc cgcgtcacgc
     1321 tcacgaacct cgactacggc gaccaggtgc tcgaggtgcg cgccgccaac tccgattcgg
     1381 tctggagcga gacgcccctg cgcttgacga tccggcgcga tccggctccg tgggtgtcgc
     1441 cgtgggcgta cgcgctctat gcgctcgtcg tcgccgcgtt cgtggggcag cgaatccgcc
     1501 gtcaccggca gaagatccgc gagatggtcg aggcgcgcgc gcgcctcgag gccgaggtgc
     1561 agctgcgcac gcgcgagctg accgagagca atcggcagct cgccgaagcc gcgcgcgcga
     1621 agagcgactt cctcgaccgc atgagccacg agctgcgcac gccgatgaac ggcgtggtcg
     1681 gcatgacgga gctcctcgcg cgcacgtcgc tgtcggccac gcagtcgcac ctcacgaaga
     1741 cgatccgctc gtcggcacag atcctgctcc agatcgtcaa cgacctgctc gatctttcga
     1801 agatccgcgc gggcaaggtg gcgctcgaga agctgcccgt ggatctcaag caagtgctcg
     1861 aggagtgcac gagcatcttc gccggcgcgg ccgattcgaa aggcatcgag ctgatcgtct
     1921 gcccgccgcc gccgcagcgg cgcgttctgc tcggcgaccc gctaaggctc cgccaggtgt
     1981 tgatgaacct ggtcggcaac gcggtcaagt tcacggagcg cggcgaggtc gtcgtgcgcg
     2041 cagacgtcga ggccgccgag ggcgaccgcg cgatcgtgcg gctcgccgtg accgataccg
     2101 gcatcggcat ggacgccgcc gcgatggaca agatcttcga gccgttcgcg caagccgacg
     2161 agacgacgac gcgccgcttc ggcggcaccg gcttggggct cgcgatctgc cgcgagctgg
     2221 ccgatctcat gaacggcgaa atcgccgtcg agagtcagcc cggcgtcggc tccacgttcc
     2281 ggctgtcgtt gcctgcggcg ctcggcgccg agatcgtgcc cg
//
LOCUS       DASSNN010000588         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_964067, whole genome shotgun sequence.
ACCESSION   DASSNN010000588 DASSNN010000000
VERSION     DASSNN010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964067"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFL84_10865"
     CDS             complement(<1..184)
                     /locus_tag="VFL84_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004530684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7609168.1"
                     /translation="MAVKRPAVFLDKDGTLIDNVPYNVDPLRVTLARGAGGALRELSE
                     HGFLLVVVSNQPGIALG"
     gene            complement(175..906)
                     /locus_tag="VFL84_10870"
     CDS             complement(175..906)
                     /locus_tag="VFL84_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011853949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7609169.1"
                     /translation="MSGVSLEGELVLVTGGGQGLGAATCGVLARAGAAVAVTDLHADR
                     AETVAREICANGGKAFALRLDVGDERTAPEALEAAAEAHGGLFALINNAGVDFTKPID
                     ELTIEQWDTVVRTNLRGPFLLCRAAAAVMKRHKRGHIVNVASTAAKRAWANASAYHAS
                     KWGLLGLSYALHAELRPHGVRVCAVVAGGMRTPFLLDRFPDLDPSKLQDPENVAETIK
                     HVLTQPPGTAIAEVMVIPTEETSWP"
     gene            complement(903..2195)
                     /locus_tag="VFL84_10875"
     CDS             complement(903..2195)
                     /locus_tag="VFL84_10875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7609170.1"
                     /translation="MPQRIAMISDHASPLAAVGGVDSGGQNIYVAETARHLAARGYLV
                     DVFTRKDQPDQPEVVDWLPGVRVVHVPAGPPAYVRKEDILQWIPDFSAFVQRFAARER
                     YDLVHAHFFMSGLVALELKSALGLPFVVTFHALGLVRRLHQGAADEFPARRIAIERDI
                     IESADAIVAECPDDHRNLVTLYNADPHKIAVIPCGFASEDFWPIAPRFARRALGLPSD
                     VPVLLSVGRLVPRKGVDNVIRALGVLVRRLGVPAELLVVGGNSDIADPALTPEIGRLR
                     AVAAEAGVAERVTFTGRRSRELLKLYYSAADVFVTTPWYEPFGMTPVEAMACGTPVVG
                     ARVGGIKYSVLDGSTGYLVPPNDPAALAEKLNEICSSRRLAERFSSVGVRRAYALFRW
                     ERVAHSLAALYADVRVAAGGLNALPAVAEPLTGVVAAA"
     gene            complement(2376..3635)
                     /locus_tag="VFL84_10880"
     CDS             complement(2376..3635)
                     /locus_tag="VFL84_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609171.1"
                     /translation="MKRHECARRLGACLVVACASVSYTAHAEWEAIPDITLEAESNDN
                     PALNTAGTTETQLIDQATRLLADASVRIRKAEPRGEISFEPRVRGDLYAEEEARILES
                     TDFFLRSNGVQRGQTVRLGYSADIARERIVGVEFLDTLPTDPVEEDPSAVATTQVGIN
                     EQRTRVGIAPFVDIAMNSRSSVVLDGRLIDVDYATDQIAGRTDFLERAIGGEYRRALG
                     DRRGTFGVRAFASGYEASANANTTDTRGVEINYSRAMTELWSWEGSIGTQRSDFALNT
                     GGRRVRGTDTTPTYAFGITKRGERSTMHSEVVRRMSPDALGFVAPRDEVRVGWRKMMS
                     PRVNGHLVLRAIDAEGIPTVLGSGRRYGRLELALDWALRPTWSFIASYAYASAESEVT
                     IGDPSESNALTLGIRYHGRSMRPSTLQ"
     gene            complement(3654..>4036)
                     /locus_tag="VFL84_10885"
     CDS             complement(3654..>4036)
                     /locus_tag="VFL84_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET7609172.1"
                     /translation="IVQAKRNDNRVTRVGRLLRRTSLDELPQLVNVLQGRMSLVGPRP
                     HAVAHNEQYRNLIAGYMVRHKVTPGITGLAQVSGCRGETSTVDEMRRRVQYDLEYLRH
                     WCWLLDIKIMLRTLVLVFRDKRAY"
BASE COUNT          646 a         1391 c         1365 g          634 t
ORIGIN      
        1 gcccgagcgc gatgcccggc tgattcgaga cgacgacgag caggaatccg tgctcggaga
       61 gctcgcgcag agcgcccccg gcgccgcgcg cgagcgtcac acgcagcgga tcgacgttgt
      121 acggcacgtt gtcgatcagc gttccgtcct tgtcgaggaa cacggcaggg cgcttcacgg
      181 ccacgacgtc tcctcggtcg ggatgaccat gacctcggcg atcgccgtgc cgggcggctg
      241 cgtcagcacg tgcttgatcg tctcggcgac gttttcggga tcctgcagct tgctcgggtc
      301 gagatccggg aaccgatcga gcaggaacgg cgtccgcatc ccgcccgcga cgacggcgca
      361 aacgcgcacg ccgtgggggc gcagctccgc gtgcagcgcg tagctcaagc cgagcaggcc
      421 ccacttcgac gcgtgatacg cgctcgcgtt ggcccaggcg cgcttggccg ccgtcgaggc
      481 gacgttgacg atgtggccgc gcttgtggcg tttcatgacg gccgcggcgg cacggcatag
      541 caagaacggg ccgcgcaaat tcgtgcgcac gaccgtgtcc cactgctcga tcgtcagctc
      601 gtcgatcggc ttcgtgaagt cgacacccgc gttgttgatc agcgcgaaca ggccgccgtg
      661 cgcttcggcc gcggcctcga gcgcctcggg agcggtgcgc tcgtcgccga cgtcgaggcg
      721 aagcgcaaaa gccttgccgc cgttggcgca gatctctcgg gcgaccgttt cggcgcggtc
      781 cgcgtgcagg tccgtaacgg cgacggcggc gccggcgcgc gccaggactc cgcacgtcgc
      841 cgcgccgaga ccctgtccgc cgcccgtgac gagcaccagc tcgccctcga gcgacacgcc
      901 gctcatgccg ccgccacgac gccggtgagc ggctcggcaa cggccggcag cgcgttcaat
      961 ccgcccgccg ccacgcgcac gtcggcgtag agcgccgcga gcgagtgtgc aacgcgttcc
     1021 cagcggaaca gcgcgtacgc gcgccgcacg ccgacgctcg agaaccgctc ggcgaggcgc
     1081 cgcgacgagc agatctcgtt gagcttttcc gcgagcgcgg cgggatcgtt cggcggcacg
     1141 aggtagcccg tcgagccgtc gagaacgctg tatttgatgc cgcccactcg ggcgccgacg
     1201 acgggcgtgc cgcaggccat cgcctcgacc ggcgtcatgc cgaacggctc gtaccaaggc
     1261 gtcgtgacga agacgtcggc cgcgctgtaa tagagcttca ggagctcgcg cgagcgcctg
     1321 cccgtgaacg tgactcgctc cgcgacgccg gcttcggcgg cgaccgcacg cagccggccg
     1381 atctccggcg tcagcgccgg atcggcgata tccgagttgc cgcccacgac gagcagctcc
     1441 gcgggaacgc cgaggcgccg cacgagcacg cccagggcgc ggatgacgtt gtcgacgcct
     1501 ttgcggggca cgagccggcc gacgctgagc agcaccggca cgtccgacgg cagcccgagc
     1561 gcgcgacgcg cgaagcgcgg cgcgatcggc cagaagtcct cgctcgcgaa accgcacgga
     1621 atgacggcga tcttgtgcgg atccgcgtta tatagggtca ccaagttgcg gtgatcgtcg
     1681 ggacattccg cgacgatcgc atcggcgctc tcgatgatgt cgcgctcgat cgcgattcgc
     1741 ctcgccggaa actcgtccgc cgcgccctga tgcagcctgc gcacgaggcc gagcgcgtga
     1801 aacgtcacca cgaagggcag ccccaaggcg ctcttgagct ccaaggcgac gaggcccgac
     1861 atgaagaaat gagcgtgcac gaggtcgtaa cgctcgcgcg cggcgaagcg ctgcacgaat
     1921 gcggagaagt ccggaatcca ttgcaggatg tcctccttgc ggacgtaggc cggcgggccg
     1981 gccgggacgt gcacgacgcg cacgcccggc agccagtcga cgacctcggg ctgatcgggc
     2041 tggtccttgc gcgtgaatac gtcgacgagg tagccgcgcg cggcgagatg ccgcgccgtc
     2101 tcggccacgt agatgttctg gccgccgctg tcgacgcctc ctacggcggc gagcggcgat
     2161 gcatggtcgc tgatcatggc tatccgttgc ggcacgtgag tttctccttg caccatcctt
     2221 ggcgaagcgc ttctggacgg ccgcaaggcg ccgtctcgcc tgaacggatg ccaccagaag
     2281 cttggctctc agcgtgcaag ccccatgcca cggccgaaaa aagccgcgcg agagaaaaaa
     2341 agtctgcggc tagcgcaaag cgcgcgctat cgggctcact gcagcgtgct cggccgcatc
     2401 gagcggccgt ggtagcggat gccgagcgtc aacgcgttcg actcggacgg atcgccgatc
     2461 gtgacttccg actcggcgct cgcgtaggca tagctcgcga tgaacgacca cgtcggccga
     2521 agcgcccagt cgagcgcgag ctcgaggcga ccgtaccgcc ggccgctgcc gagaaccgtc
     2581 ggaatgcctt ccgcgtcgat cgcgcgcagc accaggtgcc cgttgacgcg cggcgacatc
     2641 atcttcctcc atcccaccct gacctcgtcg cgcggcgcga cgaagcccaa cgcgtcgggg
     2701 ctcatgcggc gtaccacctc cgagtgcatc gtcgagcgct cgcctcgctt cgtgatgccg
     2761 aacgcgtagg tcggcgtcgt gtcggtgcct ctgacgcgcc tgccgccggt gttcaacgcg
     2821 aagtccgaac gttgcgttcc gatcgagcct tcccacgacc acagctcggt catcgcgcgc
     2881 gagtagttga tctccacgcc tcgcgtgtcg gtcgtgttcg cgttggccga cgcctcgtaa
     2941 ccgctcgcga aggcgcgcac gccgaacgtg ccccgccggt cgccgagcgc ccggcgatat
     3001 tcgccgccga tcgcgcgttc gaggaagtcg gtgcgcccgg cgatctggtc ggtcgcatag
     3061 tcgacgtcga tgaggcgccc gtcgaggacg accgagctgc ggctgttcat cgcgatatcg
     3121 acgaagggcg caatgccgac gcgcgtgcgc tgctcgttga tgccgacctg cgtggtcgct
     3181 accgcgctcg gatcctcctc cacgggatcg gtcggcaacg tgtcgaggaa ctcgacgccg
     3241 acgatgcgct cgcgcgcgat gtccgcggag taaccgagcc gcaccgtctg gccgcgctgc
     3301 acgccgttcg agcgcagaaa gaaatcggtg ctctcgagga ttcgcgcctc ttcctcggca
     3361 taaaggtcgc ctcgcacccg cggctcgaag ctgatctcgc cgcgcggctc ggccttcctg
     3421 atacgaacgc tggcgtcggc gagcagccgc gtcgcctggt cgatcaattg ggtctccgtc
     3481 gtcccggctg tgttcaaggc ggggttatcg ttcgattccg cttcgagcgt gatgtcgggg
     3541 atcgcctccc actccgcgtg cgcggtgtac gagacagacg cgcaggcgac gacgagacac
     3601 gcgccgaggc gacgtgcgca ctcgtgacgt ttcatgttga ctaacctccc tagtcagtac
     3661 gcgcgcttgt cccgaaacac gagaacgagc gtgcgcaaca tgattttgat atcgagcaac
     3721 cagcaccaat gcctgagata ttcgaggtcg tactggacgc gccggcgcat ctcgtccact
     3781 gtcgacgtct cgccgcggca gccgctgact tgcgccaagc cggtgatacc gggcgtcact
     3841 ttgtgccgca ccatgtagcc tgcgatcaga ttgcggtact gctcgttgtg agcgaccgcg
     3901 tgcggccggg ggccaacgag gctcatgcgg ccttgcagca cgttgacgag ctgcggcagc
     3961 tcgtcgagcg acgttcggcg aaggaggcgg cccacgcgcg tgacgcgatt gtcgttacgc
     4021 ttcgcctgca cgatga
//
LOCUS       DASSNN010000589         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_687477, whole genome shotgun sequence.
ACCESSION   DASSNN010000589 DASSNN010000000
VERSION     DASSNN010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687477"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFL84_10890"
     CDS             <1..466
                     /locus_tag="VFL84_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017113111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609173.1"
                     /translation="DPRDDLSGTDVARKAVILAREAGLTLELKDIAVESLVPAALKEA
                     SVEEFLKRLPDFDAPMAERVAAAKRAGQVLRYVAAIDVRAGKARVGLESFAPKHAFAS
                     ISLTDNIVQFETGRYCDNPLIIRGPGAGPAVTAGGIFADLLRLCSMLSGNHS"
     gene            472..1773
                     /gene="thrC"
                     /locus_tag="VFL84_10895"
     CDS             472..1773
                     /gene="thrC"
                     /locus_tag="VFL84_10895"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET7609174.1"
                     /translation="MRYVSTRGGQEAGLVDAVMRGLAEDGGLFVPDELPRLDASAVRG
                     ESLAEIAVEVLAPFFEGSGLENELGNICRDAFDFPLPLKELTANGELSVLELFHGPMA
                     AFKDVGARFLAALMERALRSSTDARPLTILVATSGDTGGAVAAAFHRRRGIRVGVLFP
                     KGQVSPRQQHQLTCWGDNVRAFEVDGTFDACQALVKAAFTDAGLQRRHRLTAANSINI
                     GRLLPQVAYHAAGSLWHWRRHGTAAGSIVPSGNLGNGMACIWARAMGMPVREVVLAVN
                     ANRTIPDYLATGQWQPRPSIATLASAMDVGNPSNIERLRNLLPDFAELRASIESYPVD
                     DAEIRAQIAKDYERYGEVWCPHTATGFWVYDHLPPARKAGRPWVVSATAHPAKFETIV
                     EPVIGRSVPVPPALAELLHRPTASTPLAPRLEQFAAEIDAW"
     gene            1809..>2407
                     /locus_tag="VFL84_10900"
     CDS             1809..>2407
                     /locus_tag="VFL84_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET7609175.1"
                     /translation="MTSQYQIIAGAAVLVVLAKALLRRRPAVTPVTYVIAAVGVITCG
                     AMLFWQWQDVEDHAPITTMSDMVRVDAFGVFLGVVVLGATAMSLLVAIGYLRREQLEA
                     AEYVSLMLFSATGMLVMTTANDLIVVFLALEILSIPLYVLAAYHRRRLSSQEAGIKYF
                     VLGAFSSAIFLYGIALVYGATGTTNLSAIGSHVTEASFS"
BASE COUNT          355 a          824 c          831 g          397 t
ORIGIN      
        1 cgacccgcgc gacgacctct cgggcacgga cgtcgcgcgc aaggccgtga tcctcgcgcg
       61 cgaagcgggc ctcacgctcg agctcaagga catcgccgtc gaaagcttgg tcccggcggc
      121 gctcaaggag gcttccgtcg aggaattcct gaagcgcttg cccgacttcg acgctccgat
      181 ggcggagcgc gtcgccgccg cgaagcgggc ggggcaagtg ctgcgctacg tcgccgcaat
      241 cgacgtgcgg gccgggaagg cgcgggtcgg cctcgagtcg ttcgcgccca agcacgcgtt
      301 cgcgagcatc agcctcacgg acaacatcgt gcagttcgaa accggccgct attgcgacaa
      361 cccgctgatc atccgcgggc cgggcgcggg cccggccgtc acggccggcg gcatttttgc
      421 ggacttgctg cggctctgca gcatgctgtc gggcaaccac agctagctcc aatgcgctac
      481 gtaagcacgc gcggcggtca ggaagcgggt ctcgtcgacg cggtcatgcg cgggttggcc
      541 gaggacggcg gcctcttcgt gcccgacgag ctgccgcgac tcgacgcgag cgccgtgcgc
      601 ggcgaatcgt tggccgagat cgcggtcgag gtgctcgcgc cgttcttcga gggctccggc
      661 ctagagaacg agctcggcaa catttgtcgc gacgcgttcg atttcccgct gccgttgaaa
      721 gagctcacgg cgaacggcga gctctccgtg ctcgagctct ttcacggccc gatggccgcc
      781 ttcaaggacg tcggggcgcg tttcctcgcg gcgctcatgg agcgcgcgct tcgcagctcc
      841 acggacgctc ggccgctgac gatcctcgtc gcgacgtcgg gcgataccgg cggagccgtc
      901 gcggcggcgt ttcaccggcg gcgcggcatc cgcgtcggcg tgctgtttcc gaaggggcag
      961 gtgtcgccgc gccagcagca ccagctcacg tgctggggcg acaacgtccg cgcgttcgag
     1021 gtcgacggga cgttcgacgc ctgtcaggct ctcgtcaagg cagcattcac ggacgcgggc
     1081 ttgcagcgtc gccaccggct cacggcggcg aacagcatca acatcggccg gctgctgccg
     1141 caagtcgcgt atcacgcggc gggcagcttg tggcactggc gccggcacgg cacggcggcg
     1201 ggctcgatcg tgccgagcgg caatctcggc aatggcatgg cgtgcatctg ggcgcgcgcg
     1261 atgggcatgc cggtgcgcga ggtcgtgctc gccgtgaatg cgaaccggac gattcccgac
     1321 tatctcgcga cgggccagtg gcagccgcgc ccgagcatcg cgacgctggc ctcggcgatg
     1381 gacgtcggca acccgagcaa catcgagcgg ctccgcaatc tgttgccgga tttcgcggag
     1441 ctgcgcgcga gcatcgagtc ctatcccgtc gacgacgccg aaattcgcgc gcagatcgcg
     1501 aaggactacg agcgctacgg cgaggtctgg tgcccgcaca cggcgaccgg cttctgggtc
     1561 tacgaccact tgccgccggc gcgcaaggcg gggcggccgt gggtcgtcag cgcgacggct
     1621 catcccgcga agttcgagac gatcgtcgag ccggtgatcg gccgctcggt gccggttccg
     1681 cccgcgctcg cggagctgct gcaccggccg accgcgagca cgccgctcgc gccgcgcctc
     1741 gagcaattcg cggccgagat cgacgcttgg tagctcagaa taacaatgac ttacgtggtt
     1801 aatagaacgt gacgtctcaa taccagatca tcgccggcgc cgccgtgctc gtcgtgctgg
     1861 ccaaggcgtt gttgcgccgc cggcccgcgg tcacgccggt cacctacgtg atcgccgcgg
     1921 tcggcgtgat cacgtgcggc gccatgctgt tctggcagtg gcaagacgtc gaggaccacg
     1981 cgcccatcac cacgatgagc gacatggtgc gcgtcgatgc gttcggcgtg ttcctcggcg
     2041 tcgtcgtgct cggcgcgacg gcgatgtcgc tgctcgtcgc gatcgggtac ctgcgacgcg
     2101 agcagctcga ggccgccgag tacgtgtcgc tcatgctgtt ctcggccacc ggcatgttgg
     2161 tcatgaccac cgccaacgac ctgatcgtcg tgttcctggc gctggagatc ctgtcgatcc
     2221 cgctctacgt gctcgcggcc taccaccggc ggcggctcag ctcgcaggaa gccggcatca
     2281 agtacttcgt gctcggcgcg ttctcgtcgg cgatcttcct ctacggcatc gcgctcgtgt
     2341 acggcgcgac cgggaccacg aatctctcgg ccatcggcag tcacgtcacc gaggcgagct
     2401 tctccag
//
LOCUS       DASSNN010000590         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_335074, whole genome shotgun sequence.
ACCESSION   DASSNN010000590 DASSNN010000000
VERSION     DASSNN010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335074"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VFL84_10905"
     CDS             <1..897
                     /locus_tag="VFL84_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7609176.1"
                     /translation="PGGLTPLLFAARENCLACLPVLIEGGADVNATTPDGISAVVIAL
                     INGHYDVAGALLEAGTDPNLADYTGRTALYAAIDFNTMVKSNRPAPKVLENRLTGLDV
                     ARIALEHGADVNARLKRLPPYRAKLDRGNDGMLGGGTTAFLRAAKAGDLPAMKLLLEH
                     GADPTLAPTRNGISPLMAAAGLGTSEEDTTGRQKTQAQAIEAIQLLLDLRLDINAKAN
                     DGQTAVHGAAMQGYDDVIRFLASKGAALDTPDKDGFTPLDVALGKAGGFGFSGKEGVV
                     REHTAAVLRELMSSAGSAGAAR"
     gene            978..2168
                     /locus_tag="VFL84_10910"
     CDS             978..2168
                     /locus_tag="VFL84_10910"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET7609177.1"
                     /translation="MRLDGLWDLSFSGYVAVTLALTHVTIASVTIFLHRHQAHRGLTL
                     HPLVSHFFRFWLWLTTGMITREWIAIHRKHHARCETDEDPHSPQIYGIWRVLFGGVGL
                     YQTEAGNQETLEIYGRGAPDDWIERHVYGGRHRHLGVLVMLLIDLLVFGLPGLAVYAV
                     QMVWIPFWAAGVINGVGHYWGYRNFETADTSRNIWPVGVLIGGEEFHNNHHAYGSSAK
                     FSSKWWELDIGWLYICALEMLGLASIKKVAPRASFSRASQQTIDLETLRAVVTNRFYV
                     LKLYGRRVVAPVLHGHAEGEPSFPRRMLARVRKLMIREDSGLREDPHVRQWLENAVER
                     NHTVRTVYSFLQRLKTLCAQTTGKTESDLKRLQAWCAEAEASGIRVLGDFARLLQAYT
                     PQPA"
     gene            2222..>2434
                     /locus_tag="VFL84_10915"
     CDS             2222..>2434
                     /locus_tag="VFL84_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609178.1"
                     /translation="MRLTQTLGAGSVAALCAALLATAGATQEVDATARLAALEAELAT
                     LEHDVGLLEDTKAVKRLQRAYGYYVDK"
BASE COUNT          433 a          796 c          821 g          384 t
ORIGIN      
        1 ccgggcgggc tcacgccgct acttttcgcc gcgcgcgaga actgcctcgc ctgcctgccg
       61 gtgctcatcg aaggcggcgc agacgtcaac gcgacgacgc cggacggcat cagcgccgtc
      121 gtcatcgcgc tgatcaacgg ccattacgac gtggccggtg cgctgctcga agcgggaacc
      181 gatccgaatc tagccgatta cacgggccgc acagcgctct acgccgccat cgacttcaac
      241 acgatggtca agtcgaatcg gcctgcaccg aaggtgctcg agaaccggtt gacgggcctc
      301 gacgtggcac gcatcgcgct cgagcacggc gccgacgtca acgcgcggct caagcgcttg
      361 ccgccgtatc gcgcgaagct cgatcgcggc aacgacggaa tgctcggcgg cggaacgacg
      421 gcgttcctgc gcgccgcgaa agccggcgac ttgccggcga tgaagctgtt gctcgagcac
      481 ggcgccgatc cgacgctcgc gccgaccagg aacggcatca gcccgttgat ggcggcggcg
      541 gggctcggca cgtccgagga agacacgacg gggcggcaga agacgcaggc gcaggccatc
      601 gaagccatcc agctgctgct ggacttaagg ctggacatca acgccaaggc caatgacggt
      661 caaacggccg tgcatggggc agccatgcaa gggtacgatg acgtgatccg gttcctcgcc
      721 tcgaagggcg cggctctcga tactcccgac aaggatggct tcacgccgct cgacgtggcg
      781 ctcggcaagg ccggggggtt cgggttttcg ggtaaagaag gcgtcgtgcg cgagcacacg
      841 gccgcggtat tgcgcgagct catgagttcc gcaggcagcg cgggggctgc tcgctgagcc
      901 gtgcgaacgc gctataacgg cgaatcgcgc accggcgacc cggcggtcgc agcacataac
      961 gatagggtgg gttcgatatg aggctcgacg gtttgtggga cctctcgttc tccggctacg
     1021 tcgccgtgac gctcgccttg acgcacgtca cgatcgcgag cgtcacgatc tttctgcatc
     1081 gccaccaggc gcaccgaggg ttgacgctgc atcctctcgt aagccacttc ttccgcttct
     1141 ggctctggct gacgaccgga atgatcacgc gcgagtggat cgcgatccat cgcaaacacc
     1201 acgcgcgctg cgaaaccgac gaagacccgc atagcccgca gatctacggc atctggcgcg
     1261 tgctattcgg cggcgtcggg ctctaccaga ccgaagcagg caatcaggaa acgctcgaga
     1321 tttacggccg aggcgcaccc gatgactgga tcgagcggca cgtgtacggc ggtcggcacc
     1381 ggcatttggg tgtgctggtc atgctcctca tcgatctgct ggtgttcggc ttgcccggcc
     1441 tcgcggtcta cgcagtccag atggtgtgga ttccgttctg ggcggccggc gtgatcaatg
     1501 gcgtcggcca ctattggggc taccgcaact tcgagacggc ggacacgtcg cgcaacatct
     1561 ggcccgtcgg cgtcctgatc ggcggcgaag agttccacaa caatcaccat gcctacggct
     1621 cgtcggccaa gttctcgagc aagtggtggg agctcgacat cggttggctt tacatctgcg
     1681 cgctcgagat gctgggcctc gcgagcatca agaaggtcgc gccgcgcgcg tcgttctcgc
     1741 gcgcgtcgca gcagacgatc gacctcgaga cgctgcgcgc cgtagtcacg aaccgcttct
     1801 acgtcttgaa gctctacggc cgccgcgtcg tcgcgcccgt cttgcacggc cacgcggagg
     1861 gcgagccgag cttcccgcgc cgcatgctcg cgcgcgtgcg caagctcatg atccgcgagg
     1921 acagcggctt gcgcgaggac ccgcacgtgc ggcagtggct cgagaacgcg gtcgagcgca
     1981 atcacacggt acgcaccgtc tactcgttcc tgcagcggct gaagacgctc tgcgcgcaga
     2041 cgaccggcaa aacggagtcc gatctgaagc gcttgcaagc gtggtgcgcg gaggccgaag
     2101 cgagcgggat cagagtgctc ggcgacttcg cgagactgtt gcaagcgtat acgccacagc
     2161 ccgcctgaga gcgcggcagg cccgccggcg cctggacgtc cggcggcacg gggggaaagc
     2221 catgcgtctg acccagactt tgggcgcggg aagcgttgcg gcgctctgtg ccgcgctgct
     2281 cgcaacggcc ggcgcaacgc aggaagtcga cgcgaccgcg cgcctagcgg cgctcgaggc
     2341 cgagctcgcg acactcgagc acgacgtcgg tctgctcgag gacaccaaag cggtcaagcg
     2401 gctgcagcgc gcgtacggct attacgtcga caag
//
LOCUS       DASSNN010000591         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1232039, whole genome shotgun sequence.
ACCESSION   DASSNN010000591 DASSNN010000000
VERSION     DASSNN010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232039"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2160
                     /locus_tag="VFL84_10920"
     CDS             <1..2160
                     /locus_tag="VFL84_10920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7609179.1"
                     /translation="AVVRDDWRPSLTAAGEPVRLEGDLVSPGYFASLGVRPRLGRDFI
                     AADDVTGAPRVAIVTTGFAERRFGSAEAVIDRTITLDGESVTVIGVMPEGFENALSPT
                     AEVWAPLRYLTGQPFESREWGHHLRMVGRLRADVTLEQARREMFTIGSTPVAELPRPT
                     HASMQRGLALQSLQASVTSGVRPALLVILGAVLLLLAIACANVTNILLARALARRDEL
                     ATRAALGAEAGRLVRQLFAESALLISLGGVLGVGVAALATRALVMLAPADLPRLHAIG
                     LDARVLGLALATTAVVALVAGLWPALRARDIGSPMTLRTGPRATGHTFATLRRSLVVA
                     QVALATVLLAGSGLLLRSVEQLLSVPTGFDASNVMTMRVVVPSGSGIPYEEGQAFYER
                     VLAAVRTVPGVEEAALTSLLPLSGSSLEMYAVFWESTAGSAEHAGVAFRYTVTPEWFE
                     TMGIPLMRGRLLEASDGPDAPQAILINESFAARRFAGRDPIGQRVRIGPFNRPDGPWG
                     TIVGVVGDVKQSSLAESTDAFYVPLGQWAWLDTEQSLVVKTAGEPPAFIEPIKQAIWS
                     VNPTLPLERITTMSDLVADSEAQRTFALTIFAAFGLASLLLAGVGVYGVIEGSVTERT
                     REIGVRSALGATPKRIAAFVVGQGLTLTIVGVAIGVGIAASSTRAIASLLYGIEPFDL
                     VTYAGVVALLLAVAFVACYAPAFRAARIDPAITLRAE"
     gene            2168..>3548
                     /locus_tag="VFL84_10925"
     CDS             2168..>3548
                     /locus_tag="VFL84_10925"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7609180.1"
                     /translation="MSLKASDFKYAVRLLVKRPWFTALTVVMLAGGLSITLYTFTVLH
                     VMLYRDLPLPESSSMVKIGAGSWVDIEPLDAFELAELRAGASSFSELGVYRQSRALVG
                     DSGATRSVRSVESDWSIFEFTRTPPLLGRGFVADDNSAAAEPVAVLGYETWQSVFSGD
                     AGVVGELARINGRLTRVVGVMPRGYAFPETAELWLPLGPADLAPVAYTNAGLQTYARL
                     RAGVSVEAAQTELTAIVQRVREQRPTTGRQNSDPVAVLRFKEQGIVGAVMFGVLNLLA
                     ASILLLAAVNVGNLLLARTNERIKEIGVRISLGAPRTRLIVQITLENAILCAIGGLVA
                     LFVTARALELMNGFMRAAFENLPFWWTWGLDSGVVAAAGVALLLTVLVVSVLPALCVS
                     SVDPIALLRDGTGAGQGRGSGRVSRELVTVQIALISGIIVVGGAAAVIADRGARFEYG
                     MDTDRLLSMR"
BASE COUNT          462 a         1204 c         1248 g          634 t
ORIGIN      
        1 gccgtcgtgc gggacgattg gcgaccttcg ctcacggcgg ccggtgagcc cgtgcggctc
       61 gaaggcgatc tcgtgagtcc cggctatttc gcctctctcg gcgtacggcc ccggctcggg
      121 cgcgatttca tcgccgcaga cgacgtcacg ggagcgccgc gcgtcgcgat cgtcacgact
      181 ggcttcgccg aacgccgctt cgggagcgcc gaggccgtga tcgaccgcac gatcacgctc
      241 gacggcgaga gcgtcacggt gattggagtc atgccagagg gcttcgagaa cgctttgagc
      301 ccgacggccg aagtctgggc gccgctgcgt tatctcaccg ggcagccgtt cgagagccgc
      361 gagtggggcc accatttgcg aatggtcggc cgcctgcgcg cagacgtgac gctcgagcag
      421 gcgcggcgcg agatgttcac gatcggcagc acgccggttg ccgagcttcc gcgccccacg
      481 catgcgtcga tgcagcgcgg cctcgcgctt cagtcgctgc aagcctcggt gacgagcggc
      541 gtgcgcccgg cgctgctcgt gatcctcggc gccgtgctgc tgctgctcgc gatcgcctgc
      601 gccaacgtca cgaacatcct gctcgcgcgg gcgctcgcgc ggcgcgatga gctcgcgacg
      661 cgcgcagcgc tcggagcgga ggcggggcgc ctcgtgaggc agctcttcgc cgagagcgcg
      721 ctgctgatct cgctgggcgg cgtgctcggc gtcggtgtcg ccgcgctcgc gacccgtgcg
      781 ttggtcatgc tcgcgccggc cgacttgcca cgcttgcacg ccatcgggct cgatgcgcgc
      841 gtgctgggtt tggcgctcgc gacgactgcc gtcgtggcgc tcgtagccgg tctatggccg
      901 gcgctgcgcg cccgcgatat cggctcgccc atgacgctgc gaacggggcc gcgcgccacg
      961 ggccatacgt tcgccacgct gcgccggtcc ctcgtcgtcg cccaggtcgc gctagctacg
     1021 gtgctgctcg cgggttccgg tctgctgctg cgcagcgtcg agcagctcct ctcggtgccg
     1081 acgggcttcg acgcttcgaa cgtgatgacg atgcgggtcg tcgtgccgag cggctcgggc
     1141 attccctacg aggagggaca ggctttctat gaacgcgtgc tcgcggccgt tcgcacggtg
     1201 cccggcgtcg aggaggccgc gctgacgagc ctgctgcctt tgagcggtag cagcctcgag
     1261 atgtatgccg tcttctggga gtcgacggca ggtagcgccg agcacgcggg cgtcgcgttt
     1321 cgttacaccg tcacaccgga gtggttcgag accatgggca tacccttgat gcgtggacgg
     1381 ctgctcgaag cgagcgatgg gccggacgcg ccgcaggcga ttctgatcaa cgagtcgttc
     1441 gcggcacgcc gtttcgccgg ccgcgatcct atcggccagc gcgttcgcat aggccccttc
     1501 aaccgtccgg atgggccgtg ggggacgatc gtcggcgtcg tcggcgacgt gaagcaatca
     1561 tcgctcgcgg agtccacgga cgcgttctac gtcccactgg gccaatgggc gtggctcgac
     1621 accgagcaat cgctcgtcgt gaaaacggcg ggcgaaccgc cggcgttcat cgagccgatc
     1681 aagcaggcga tttggtccgt gaatccgacg ctgcccctcg agcgcatcac cacgatgtcc
     1741 gacctcgtcg cggactccga agcgcagcga acgttcgcgt tgacgatatt cgccgcattc
     1801 gggcttgcgt cgctgctgct ggccggcgtc ggtgtctacg gcgtcatcga gggcagcgtc
     1861 accgagcgca cgcgcgagat cggcgtccgg tcggcgctcg gggccacgcc gaagaggatc
     1921 gccgcgttcg tggtcggtca aggcctgacg ctaacgatcg tgggtgtcgc tatcggcgtc
     1981 ggcattgccg cgagctcgac gcgagcgatc gcgtcgctgc tgtacggcat cgagccgttc
     2041 gatctcgtga cgtatgccgg cgtcgtcgcg ttgctgctcg ccgtcgcgtt cgtcgcgtgc
     2101 tacgcgccgg ccttccgcgc cgcgcgcatc gatcccgcca ttacgttacg cgcggaatga
     2161 gcgagcaatg tcgctgaagg cttccgactt caaatacgcc gtgcgcctgc tcgtgaagcg
     2221 cccgtggttc acggcgctga ccgtcgtcat gctcgcgggc ggcctctcca tcacgctgta
     2281 tacgttcacg gtcctgcacg taatgctcta tcgcgacttg ccgttgcccg agagcagctc
     2341 gatggtgaag atcggcgccg gcagctgggt cgacatcgag ccgctcgacg cgttcgagct
     2401 cgccgagctg cgcgccggtg cgagcagctt cagcgagctc ggcgtttacc gccagtcgcg
     2461 cgcgctcgta ggcgacagcg gcgcgacgcg cagcgttcgc tccgtcgaat ccgattggag
     2521 catcttcgag ttcacgcgca cgccgccgct gctcggccgc gggttcgtcg ccgacgacaa
     2581 ttcggccgcg gccgagcccg tcgccgtctt gggctacgag acatggcaga gcgtcttctc
     2641 gggtgacgcg ggcgtcgtcg gcgagctcgc ccgcatcaac ggccggctca cgcgcgtcgt
     2701 cggcgtcatg ccgcggggct acgcgtttcc cgagaccgcc gagctttggc tgccgctcgg
     2761 gcccgccgac ctcgcgccgg tcgcgtacac gaatgcgggg ctccagactt acgcgcggct
     2821 tcgagccggc gtttcggtcg aggcggcgca gaccgagctg acggcgatcg tacagcgcgt
     2881 gcgcgagcag cggccgacga cgggccggca gaactccgat cctgtcgcgg ttctgcggtt
     2941 caaggagcag ggcatcgtcg gcgccgtcat gttcggcgtc ctgaacctgc tggcggcgtc
     3001 gatcttgctg ctcgccgccg tcaacgttgg gaacctgctg ctcgcgcgca cgaacgagcg
     3061 catcaaggag atcggtgtcc gaatttcgct cggcgcgccg cgcacgcggc tcatcgtgca
     3121 gatcacgctc gagaacgcga ttctttgcgc gatcggcggg ctcgtcgcgc tgttcgtcac
     3181 ggcgcgcgcg ctcgagctca tgaacggctt catgcgcgct gcgttcgaga acctgccgtt
     3241 ctggtggacg tgggggctcg actccggcgt cgtggcggca gcgggggtcg ccttactgct
     3301 gacggttctc gtcgtttcgg tgctgccggc gctctgcgtg agcagcgtcg atccgatcgc
     3361 gctgctccgc gacggcaccg gcgcgggcca ggggcgcggc tcgggtcgcg tctcgcgcga
     3421 gctcgtcacg gttcaaatcg cgctgatctc cggaatcatc gtcgtcggcg gcgccgcggc
     3481 cgtcatcgcc gatcgtggcg cgaggttcga gtacggcatg gacacggacc gccttctatc
     3541 gatgcgct
//
LOCUS       DASSNN010000592         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_101018, whole genome shotgun sequence.
ACCESSION   DASSNN010000592 DASSNN010000000
VERSION     DASSNN010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101018"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..354)
                     /locus_tag="VFL84_10930"
     CDS             complement(112..354)
                     /locus_tag="VFL84_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007636214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HET7609181.1"
                     /translation="MTAPRLSLPLRLLVLAGAISAVGGCSIEPWVKPYERDHLADPIM
                     SFDRDPVSSAYTGHVYEAREGARGATGGGGGGCGCN"
     gene            complement(359..850)
                     /locus_tag="VFL84_10935"
     CDS             complement(359..850)
                     /locus_tag="VFL84_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET7609182.1"
                     /translation="MLRAFSVFALTLLVASLAYAATVSGPAPNFTLQSNAGGQVSLAS
                     LKGKVVMVNFWATWCVPCRQEMPHLQALYEKYNGLGFELLAVNVEKNNAEGARKWLQE
                     TPVTFPVLFDPENQVTKLYKVQTMPSTVIIGRDGTMRFMHNGYKPGYENDYQTQVRAL
                     LRE"
     gene            complement(872..1531)
                     /locus_tag="VFL84_10940"
     CDS             complement(872..1531)
                     /locus_tag="VFL84_10940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HET7609183.1"
                     /translation="MESRVLVLFLGLTLGVGSLTGCARGRAAVAEDTSQPPVIEPDVA
                     RREIEPPRIDTEDFEVGITLGQISVEDFGVNTVAGARFAYHVTEGFFVEIAGGKADTE
                     LTSFERLSGAAQLLTDSEREYSYYNVSLGYNIFPGEHFIGKNRAMNTQTYVIGGVGKT
                     EFAGDDRFTINFGIGMRLMPLDWLAVHGDIRDHVFDIDLLGQEKTSHNLEATLGVTFF
                     F"
     gene            complement(1501..2244)
                     /locus_tag="VFL84_10945"
     CDS             complement(1501..2244)
                     /locus_tag="VFL84_10945"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET7609184.1"
                     /translation="MRWVLFVLSLCQLAITLNAAGAERQPREVTIADPFIELHTGPGK
                     GYPVFFVAERGEQIALLKRRTEWFEVQAARGQKGWVHFEQLATTLDADGEPFDLPALG
                     FSDYEARRWEVGALYGDFGGANVIGLYGSRTVTPNLSGEVWVSQALGRFSDSTLVTLN
                     IVHLMYPDWRASPYFTLGGGVIKTQPKATLVATTDRTDNFALAGAGVRMYLTRRFVFR
                     AEYKTHVVFTSRDDNEEVQEWKAGFSFFF"
     gene            complement(2245..>2631)
                     /locus_tag="VFL84_10950"
     CDS             complement(2245..>2631)
                     /locus_tag="VFL84_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609185.1"
                     /translation="NAAMVPAGQLDLSDGVSPDEADHFNSYQELLFGDNRQILVGGAI
                     QDEQQVVGTDAMGNPILDFVPVVPPMSPAGARQSRFFSCFDVGGTGCPSRSHVGYMSA
                     DELRLLAEWLDIGAQYYNNPFDAPVM"
BASE COUNT          482 a          884 c          821 g          444 t
ORIGIN      
        1 taggagtgca gcaggacgtc ggcgcgatcc tcgggcaaca cgcccgcctg cgccgcgcga
       61 acgacgacca gcgacaacaa ggcgatgagg agactgcgcg tcacgttggc ctcaattgca
      121 gccgcaaccg ccgccaccgc cgccggtggc accgcgcgcg ccttcgcgcg cctcgtagac
      181 gtgtcccgtg tacgcgctcg agacggggtc tcgatcgaac gacatgatcg ggtccgcgag
      241 gtggtcgcgc tcgtacggct tcacccacgg ctcgatgctg cagccgccga cggcagagat
      301 agcgcccgcc agcaccagca agcgaagagg cagcgacaat ctcggggcgg tcatcggctt
      361 actcccgcag cagggcccgg acctgggttt gatagtcgtt ctcatagccc ggcttgtagc
      421 cgttgtgcat gaaccgcatc gtaccgtcgc gtccgatgat cacggtgctc ggcatcgtct
      481 gcaccttgta gagcttcgtg acctggttct cggggtcgaa cagcaccggg aacgtcacgg
      541 gcgtttcttg cagccacttg cgagcgccct cggcgttgtt cttctcgacg ttgacggcga
      601 gcagctcgaa gcccaagccg ttgtacttct cgtagagcgc ttgcaagtgc ggcatctcct
      661 gccggcacgg cacgcaccac gtcgcccaga agttgaccat gacgaccttg cccttcagcg
      721 acgcgagcga gacctgcccg cccgcgttcg attgcagcgt gaagttgggc gccgggccgg
      781 acacggtcgc ggcgtaagcg agcgacgcca cgagcagggt caacgcgaaa actgagaacg
      841 cgcgcaacat gtagctacct ccgcaattcg cctagaagaa gaacgtgacg ccgagcgtgg
      901 cttcgaggtt atgcgacgtc ttctcctgcc ccaacaagtc gatgtcgaaa acatggtcgc
      961 gaatgtcgcc gtgcacggcg agccagtcga gcggcatcaa ccgcatgccg atgccgaaat
     1021 tgatcgtgaa ccgatcgtca ccggcgaact cggtcttgcc gacgccgccg atgacatacg
     1081 tctgtgtgtt catcgcgcga ttcttaccga tgaagtgctc gccagggaaa atgttgtaac
     1141 ccagcgacac gttatagtag gaatactccc gctcggagtc tgtgagcaac tgcgcagcgc
     1201 cgctcagccg ctcgaagctc gtgagctccg tgtcggcctt gccgcccgcg atctcgacga
     1261 aaaacccttc cgtcacgtgg tacgcgaagc gcgcgcccgc gaccgtgttg acgccgaaat
     1321 cctcgacgct gatctgcccg agcgtaatgc cgacctcgaa gtcttcggtg tcgatgcgcg
     1381 gcggctcgat ctcgcggcgc gcgacgtcgg gctcgatgac cggcggctgc gacgtgtcct
     1441 cggcgaccgc agctcggccg cgggcgcagc ccgtgaggct tccgacgccg agcgtcaggc
     1501 ctagaaaaag aacgagaacc ctgctttcca ttcctgcact tcctcgttgt cgtcacggct
     1561 cgtgaacacg acgtgggttt tatattcggc ccggaacacg aacctgcgag tcaaatacat
     1621 gcggacgccg gcgcccgcga gagcgaagtt gtcggtcctg tcggtcgtcg cgacgagcgt
     1681 cgccttcggc tgcgtcttga tcacgccgcc gccgagcgtg aaatacggcg acgcacgcca
     1741 gtccgggtac atgagatgca cgatgttcag cgtcacgagc gtgctgtcgg agaagcgccc
     1801 gagcgcttgc gacacccaga cctcgcccga caggttcggc gtgacggtgc gcgagccgta
     1861 caggccgatg acgttcgcgc cgccgaagtc gccgtacaac gctccgactt cccaacgccg
     1921 cgcttcgtag tcgctgaagc cgagcgccgg cagatcgaac ggctctccgt cggcatcgag
     1981 cgtcgtcgcc agctgctcga agtgcaccca gcccttctgg ccgcgcgccg cctggacctc
     2041 gaaccactcc gtgcgccgct tcaagagcgc gatctgctcg ccgcgctcgg ccacgaagaa
     2101 cacgggatag cctttgccgg ggcccgtgtg cagctcgatg aacggatcgg cgatcgtcac
     2161 ctccctaggt tgccgttctg cgcctgccgc attgagcgtg atcgcgagct ggcagagcga
     2221 aagaacgaac aacacccatc gcatctacat caccggcgca tcgaacgggt tgttgtagta
     2281 ctgcgcgccg atgtcgagcc attccgccaa aagccgcagc tcgtcggccg acatgtagcc
     2341 gacgtgcgag cgcgacggac accccgtgcc gccgacgtcg aagcacgaga agaaccgcga
     2401 ctgccgcgcg ccggccgggc tcatcggcgg aacgaccggg acgaagtcca ggatcggatt
     2461 acccatcgcg tccgtgccga cgacctgctg ctcgtcctgg atcgcgccgc cgacgaggat
     2521 ctggcggttg tcgccgaaca agagctcctg atacgagttg aaatggtccg cctcgtcggg
     2581 cgaaacgccg tcgctcaagt cgagctggcc cgccggcacc atcgccgcgt t
//
LOCUS       DASSNN010000593         4443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_605720, whole genome shotgun sequence.
ACCESSION   DASSNN010000593 DASSNN010000000
VERSION     DASSNN010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605720"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1826)
                     /locus_tag="VFL84_10955"
     CDS             complement(<1..1826)
                     /locus_tag="VFL84_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609186.1"
                     /translation="MLKRIAASCLALVLATGVRGQDVAPAEATSPVINRAVMMGDEVV
                     SLLDETVDWYRMLGVQQQSATQPSDLLFLYANRQAADRVVALAFELARANAELLSSQA
                     EVQQDAQADASGSPQALQRQQLDLDARAASLEAEIAAAQRQLGAAKGEDRSMMQARVA
                     ELQGDLDVVNARRNLLNNMMELAHETDANGSGVTALKEHINAIAASIPAASGVMPELG
                     ATEAASAVSAAFASADQIGARRFGIWDLGRNVIRLQNKLATIDEVDRRTEELQQTFMQ
                     SRAAPEEQIAMLSSRGDELAARSGGSDAAAMKSARNEYDTIAWLFAQTSSILVPLTKA
                     GVLLDQYRSNLDGWRAATRTQYCAALRTLAARLTFLLLLLAVVFAGGEIWKRAVFRYV
                     QETRRRHQLLLVRRIVVWAIAIVIIGIALSTEASSFATFAGLLTAGIAVAMQSVLVSI
                     VGYFFLIGKYGLRVGDHVQIGSVSGEVIDLGLVRLHLMELGPQHGPTGRVVALANSVV
                     FQASGGIFKQIPGVSLAWRELSLTLPGDADYAAIKERLLAAASRVLENYGEELERQTR
                     ALNKTSWGNMDKVEAQVQLRFSSDSIEAIVRYPVPLQRAAEV"
     gene            1998..2861
                     /locus_tag="VFL84_10960"
     CDS             1998..2861
                     /locus_tag="VFL84_10960"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HET7609187.1"
                     /translation="MYAVVGATGNSGRAVVKELTGLGEKPVAVVRNADKAREVLGPSA
                     KIAIAELDDRAAMTKALAGAKRVFIVTGHNPSSAAQQINVIEAAKAAGAEFIVKVSGG
                     RAVTGPNVESQNGRAHYEIEEHLKRCGLQWCILSPGLFMQNMLGQAANIKQGKIAQPW
                     AKDLPVALIDVRDTGALGARVLRDPAKHAGKVYSFTGAATTYGELANVISEALGKPIT
                     YTELTLEQAEQGMKARGMPDWLVAHLLAMARAGAGGAFSKENTQPIRDIVGRAPITIR
                     QFVQDFKGAFV"
     gene            2888..3286
                     /locus_tag="VFL84_10965"
     CDS             2888..3286
                     /locus_tag="VFL84_10965"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609188.1"
                     /translation="MSRGLSRACIAAAALLTASAALAHHSFAMFDQSTTVTLAGTVKE
                     FRWTNPHVFIEMLVKNDGGAEEQWSIEMTSPEHLARAGWRPSTLKPGDALTLSIHPMR
                     DGIKGGQYLSGIGPKGPLAGSRFAPPEPTR"
     gene            3283..4098
                     /locus_tag="VFL84_10970"
     CDS             3283..4098
                     /locus_tag="VFL84_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609189.1"
                     /translation="MSFRRAAGAAAALLLACGCDTRVAQLPDWSGQWENVGATPSPDG
                     GFYESLEQVLNELEWSPPLKPESQAAIDRVVEVQRKAMQAMRDGIDPGGAVTACTFGF
                     PTIMLESPLMFEILPTPKETALIFSSREIRHVYTDGRDHPPEGDVWPTPWGDSVGHWE
                     GDTLVIDTIAVKSAFPGREGSAVIAFGGIEESQMIAFLSPEAHFVERIRMLDNGQLEN
                     RMTITDPANLTEPWHMTRTYRRETHIDRMVHEDCEGEERNPIVDGKFTLTPPP"
     gene            4193..>4443
                     /locus_tag="VFL84_10975"
     CDS             4193..>4443
                     /locus_tag="VFL84_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609190.1"
                     /translation="MIALCLAASAGACVQSPGGDPAEQSANTSSEPSAPLMTVNQLMR
                     GILFPLGNTVFYAQADDPAAVPRDPMPSASTNPLTGIFGG"
BASE COUNT          800 a         1473 c         1466 g          704 t
ORIGIN      
        1 tcgacctccg ccgcacgctg cagcggaacg ggataccgga caatcgcttc gatggaatcg
       61 gacgagaacc gcaattgcac ttgcgcttcc accttgtcca tgttgcccca cgacgtcttg
      121 ttcagcgcgc gcgtctgccg ctcgagctcc tcgccatagt tctcgagcac gcgggatgca
      181 gcggcgagca gccgctcctt gatcgccgca tagtcggcgt cgcccgggag cgtaaggctc
      241 agctcgcgcc acgcgaggct tacgccggga atctgcttga aaatccctcc cgacgcttgg
      301 aacacgaccg agttcgcgag cgcaacgact cgtccggttg ggccgtgttg cggcccgagc
      361 tccatcaagt gcagccgcac caaaccgaga tcgatgactt caccggatac gctgccgatc
      421 tggacgtgat caccgacgcg caggccgtat ttaccgatca ggaaaaagta gccgacgatc
      481 gacaccagca cgctctgcat cgcgacggcg atgccggcgg tgagcaggcc ggcgaaggtg
      541 gcgaacgagc tcgcctcggt cgagagcgca atgccgatta tcacgatggc aatggcccag
      601 acgacgattc ggcgcacgag cagcagctgg tgccggcgcc gtgtctcctg cacgtacctg
      661 aacaccgccc gcttccagat ctcgccgccg gcaaacacga cagcgagcaa caagagcaga
      721 aacgtcaagc gcgcggccag cgtcctcaac gccgcacagt actgtgttcg ggttgcggcg
      781 cgccaaccgt cgagattgct ccggtactga tccagcaaca cgccggcttt cgtgagaggc
      841 acgagaatcg atgacgtctg tgcgaagagc cacgcgatcg tgtcgtattc gttgcgcgcg
      901 gacttcattg cggcggcatc gcttccgccg gaccgtgccg cgagctcgtc gccgcgcgag
      961 gacagcatgg cgatctgctc ctcaggcgcc gctcggctct gcatgaatgt ctgctgaagc
     1021 tcctcggtgc ggcggtccac ttcgtcgatc gtcgcgagct tgttctgcag cctgatcacg
     1081 ttgcggccga gatcccaaat gccgaaccgc cgcgcgccga tctgatcggc ggacgcgaac
     1141 gcagcgctga cggctgaggc cgcttccgtt gcgccaagct ccggcatcac gcccgaagcg
     1201 gccggaatcg aggccgcaat cgcgttgatg tgctccttga gggcggtcac acctgacccg
     1261 ttcgcgtccg tttcgtgcgc gagctccatc atgttgttca ggagattgcg ccgcgcgttc
     1321 acgacgtcga ggtcgccctg cagctccgcg accctcgctt gcatcatgct gcgatcctcg
     1381 cccttcgccg cgccgagctg tcgttgcgcg gccgctattt ccgcctcgag gctggccgcg
     1441 cgggcgtcga gatcgagttg ctgtcgctgc agcgcctgcg gcgaaccgga agcgtcggcc
     1501 tgagcatctt gttgcacctc tgcctggctg ctgagtagct cggcattcgc gcgcgcaagc
     1561 tcgaacgcga gagcgacgac gcggtcggct gcctgccggt tggcatagag gaagagtaag
     1621 tcgctcggct gagtggcgga ttgctgctga acgccgagca ttctgtacca gtcgaccgtc
     1681 tcgtcgagaa ggctgacaac ctcgtcgccc atcatcacag cgcggttgat gaccggagac
     1741 gtggcttccg ccggcgcgac atcctgccct cggactcccg tcgcgaggac cagggcaaga
     1801 cacgatgcgg caattcgctt caacatgacg gcaggcggtc cgatcgaggg agcagatggt
     1861 acccggttgg acgtgagggg cccattggcc tcgaagccca tggcagggcc gccggcaacg
     1921 ggcgtgctag catcgagccg ctcgcacctg aagccgaata tcgctcggag cgccccgcgc
     1981 tccaatggag gacggtcatg tacgcggtcg ttggcgcaac cggaaattcc gggcgcgcag
     2041 tcgtcaagga gctcacgggc ctcggcgaga agccggtggc cgtcgttcgc aacgcggaca
     2101 aggcgcgcga ggtgctcgga ccgagcgcca agatagcgat cgccgagctc gacgaccgcg
     2161 ccgccatgac gaaggcgctc gcgggcgcaa agcgcgtgtt catcgtcacg ggccacaacc
     2221 cgagctcggc cgcgcagcag atcaacgtga tcgaagcggc gaaggcggcg ggcgcggaat
     2281 tcatcgtcaa ggtttcgggc ggccgcgccg tgacggggcc gaacgtcgag tcgcagaacg
     2341 gccgcgcgca ttacgagatc gaggagcacc tgaagcgctg cggactgcag tggtgcattt
     2401 tgagcccggg tctcttcatg cagaacatgc tggggcaggc agcgaacatc aagcagggca
     2461 agatcgcgca accatgggcg aaagaccttc cggtagcgct gatcgacgtg cgcgacaccg
     2521 gcgcgctcgg cgcacgcgtg ctgcgcgatc cggcgaagca cgccggcaag gtctattcgt
     2581 tcaccggcgc cgcgacgacg tacggcgagc tcgcgaacgt catctccgag gcgctcggca
     2641 agccgatcac ctacacggag ctcacgctcg agcaggcgga gcaaggcatg aaagcgcgcg
     2701 gcatgcccga ctggctcgtg gcacacctgc tcgcgatggc gcgagccggt gccggcggcg
     2761 cgttctcgaa agaaaacacg cagccgatcc gcgatatcgt cggccgcgcg ccgatcacga
     2821 ttcgccagtt cgtgcaggat ttcaaaggcg cgttcgtctg aacggacagc ctgcgacgga
     2881 gacgcgcatg agccggggtt tgagtcgagc atgcatcgct gccgcggctc tgctgaccgc
     2941 gagcgccgcg ctcgcgcacc attcgttcgc gatgttcgac cagtccacga cggtcacgct
     3001 cgcgggcacc gtcaaggaat tccgctggac caatccgcac gtcttcatcg agatgctcgt
     3061 caagaacgac ggcggcgccg aagagcaatg gagcatcgag atgacgagcc cggagcatct
     3121 cgcgcgcgcg ggctggcggc cgtcgacgct gaagcccggt gacgcgctga cgctttccat
     3181 ccatccgatg cgcgacggca tcaaaggcgg ccagtacctt tccggcatcg ggccgaaggg
     3241 cccactggcc ggctcgcggt tcgcgccgcc ggagccgacg cggtgagctt ccggcgcgca
     3301 gcgggagcgg cggccgcgct gctgctcgca tgcggttgcg acacgcgcgt cgcgcaatta
     3361 cccgattgga gcggccagtg ggagaacgtc ggcgcgacgc cgagcccgga cggcggcttc
     3421 tacgaatcgc tcgagcaagt gctgaacgag ctcgagtggt cgccgccgct caaacccgaa
     3481 tcgcaggcgg cgatcgaccg cgtcgtcgaa gtgcagcgca aagcgatgca ggcgatgcgc
     3541 gacggcatcg acccgggcgg cgcggtcacg gcgtgcacgt tcggctttcc gacgatcatg
     3601 ctcgagtcgc cgttgatgtt cgagattttg ccgacgccga aggagaccgc gctgatcttc
     3661 tcgagccgcg agatccgcca cgtctacacg gacggccgcg accacccgcc cgaaggcgac
     3721 gtctggccga caccgtgggg cgactcggtc ggccactggg aaggcgacac gctcgtgatc
     3781 gacacgatcg ccgtcaagtc cgcgtttccg ggacgagaag gctcggccgt gatcgcgttc
     3841 ggtggaatcg aggaatcgca gatgatcgcg ttcttgagcc ccgaggcgca cttcgtcgag
     3901 cggatccgca tgctcgacaa cgggcagctc gagaatcgaa tgacgatcac cgatccggcc
     3961 aatctgaccg agccttggca catgacgcgc acgtaccgcc gcgagacgca catcgaccgc
     4021 atggtgcacg aggactgcga gggcgaggag cgcaatccga tcgtcgacgg caaattcacg
     4081 ctgacgccgc cgccctaacc cgccggcacg gttccggacc gcaatgcgct aagctcacgc
     4141 gaatcagtcg cggggggatg tttgcatgtc gaatcgtagg cgatggccca acttgatcgc
     4201 tctgtgtctc gccgcctcag ccggcgcttg cgtgcagtcg ccgggcggcg atcccgcgga
     4261 acagagcgcg aacacttcga gcgagccgtc cgcgccgctc atgaccgtca accagctcat
     4321 gcgcggcatt ctgttcccgc tcggcaacac ggtgttctac gcgcaggccg acgaccccgc
     4381 cgcggtgccg cgggacccga tgccgtcggc gtcaacgaat cccttgacgg gaatcttcgg
     4441 cgg
//
LOCUS       DASSNN010000594         4790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_231706, whole genome shotgun sequence.
ACCESSION   DASSNN010000594 DASSNN010000000
VERSION     DASSNN010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231706"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1839
                     /gene="recG"
                     /locus_tag="VFL84_10980"
     CDS             <1..1839
                     /gene="recG"
                     /locus_tag="VFL84_10980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET7609191.1"
                     /translation="TDGTGAVTLRFFYFNKSVEQALARGRRVRCYGDVRSGPAGLEMV
                     HPEHRAVTAEDSEPSDRLTPVYPTTEGLYQQTLRRVVERAVAALEHEKLEDYLAPALK
                     LRSLAGEPWPPLATALAYLHSPPRDAATELLLSRRDACSRRIALEELVAQRLSLRRAA
                     ALTRSERARPLPTPAERLAQFRAALPFDLTRAQTAAIDEIVADLRKDAPMNRLLQGDV
                     GSGKTVVAAMAALVAAAAGCQTAVMAPTELLAEQHFASFERWLTPLGIEVAALIGAQP
                     ARARAATLGAVADGRAAVVVGTHALFQESVEFRRLALVVVDEQHRFGVEQRRRLKQKG
                     ADDARVPHQLIMTATPIPRTLAMTAYADLDCSVIGELPPGRQPVHTAVVPEQRRAELV
                     RRVAAHCAAGQQAYWVCPLIEESELVDSRAASALHAELAEALPNARVGLIHGRMKSAE
                     KDAVMRAFKARELDLLVATTVIEVGVDVPNATLMVVENAERMGLAQLHQLRGRVGRGA
                     KASTCMLLYRAPLSDLARQRLGVLRETNDGFAVAQKDLELRGPGEVLGTRQTGVMQLR
                     IADLLRDADLLPQVIEISDQLLATAPEAADALIRRWIRGEAEYAKV"
     gene            1943..2827
                     /gene="ubiA"
                     /locus_tag="VFL84_10985"
     CDS             1943..2827
                     /gene="ubiA"
                     /locus_tag="VFL84_10985"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HET7609192.1"
                     /translation="MAWGHAQALLAHYARYWRLMRLHRPIGIWLLLWPTLWALWIASA
                     GRPDPKVFVFLVLGTIVVRSAGCVINDFVDRKIDPHVARTADRPLATGEVAPVEALLL
                     FGALMLIALGLVLNLNRLTLTFAAAGAFLTIVYPFTKRFLSTPQFVLGVAFSWGVPMA
                     FAATAGDVPRVGWLLFLSTVIWVVVYDTQYAMTDRPDDIKLGVRSTAILFGELDRTFI
                     VGLQALFLASLVLVGRSAAMGPWYYGGLGVACVFCLYQAYLIKERDIVQSFRAFLNNA
                     WLGGAIFAGIVLDYTFRP"
     gene            complement(2809..3498)
                     /locus_tag="VFL84_10990"
     CDS             complement(2809..3498)
                     /locus_tag="VFL84_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET7609193.1"
                     /translation="MVYGIFDRLFTESCPACGLASRGGFCGVCAAQLPRIARACRRCG
                     LAEPVAHCPRKETAWDVDAVIAPFSYRPPLDHYVQAMKYRGARAMGRAFALLLMPSLA
                     EQDGVDALAAVPLHGSRLRERGYNQAQEIARVLARALGLPAMMRGIARPVATPKQAGQ
                     GMLERRRSVARAFRVSCDLRGRHIAIVDDVVTTGATVNALAVELKAAGAERCVVLAVA
                     RTPEPAQGRNV"
     gene            3583..4635
                     /gene="bioB"
                     /locus_tag="VFL84_10995"
     CDS             3583..4635
                     /gene="bioB"
                     /locus_tag="VFL84_10995"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HET7609194.1"
                     /translation="MSAIPRDTALDPPVAAGPRADWTRPEVEALFELPFNDLLFKAQS
                     VHRQTFDPNAVQVSTLLSIKTGACPEDCAYCPQSIRFETGVDTHDLMPLEQVRAAAQR
                     AKDAGATRFCMGASYRGPKDSQLEPILEMVKAVKSVGLETCATLGLLRPGQAERLAEA
                     GLDYYNHNLDSSAEFYGKIISTRTYADRLETLERVRAAGVKVCCGGILGMGETRADRV
                     GLLHTLATLVPQPESVPINELVPIPGTPLASAEPLDPLEFVRCIAVARILMPRSHVRL
                     SAGRSDFSDELQALCFFAGANSIFYGEKLLTTGNPDTAADRRLFDRLGLAPEGTGTDA
                     RGRAAQNAVPEGLAAS"
BASE COUNT          678 a         1686 c         1693 g          733 t
ORIGIN      
        1 accgacggca ccggcgcggt cacgctgcgg ttcttctatt tcaacaagtc cgtcgagcaa
       61 gcgctcgcgc gcggtcggcg cgtgcgctgc tacggcgacg tgcgcagcgg gcccgccggg
      121 ctcgagatgg ttcatcccga gcaccgcgcc gtgaccgccg aggattcgga gccgagcgac
      181 cggctgacgc ccgtctaccc gacgacggaa ggcctgtatc agcaaacgtt gcgccgcgtc
      241 gtcgagcgcg cggtcgccgc gctcgagcac gagaagctcg aggactacct cgcgccggcg
      301 ctgaaactcc ggtcgctggc cggtgagccg tggccgccgc tcgcgacggc gctcgcctat
      361 ctgcattcgc cgccgcgcga cgccgcgacg gagctgctcc tgtcgcggcg tgacgcgtgc
      421 tcgcggcgca tcgcgctcga ggagctcgtc gcgcaacggt tgagcctgag gcgcgcggcg
      481 gcgctgacac gctccgagcg ggcccggccc ctgccgacgc cggccgagcg gctcgcccaa
      541 tttcgcgcgg cgctgccttt cgacctcact cgcgcgcaga ccgcggcgat cgacgagatc
      601 gtggcagatc tgcgcaagga cgcgccgatg aaccggctgc tgcaaggcga cgtcggctcg
      661 gggaagaccg tcgtcgccgc gatggccgcg ctcgtcgccg ccgccgcggg atgccagacg
      721 gccgtcatgg cgccgacgga gctgctcgcc gagcagcact tcgcgagctt cgagcgttgg
      781 ctcacgccgc tcggcatcga ggtcgcggcg ctgatcggcg cacagcccgc gcgcgcgcgc
      841 gccgcgacgc tcggcgccgt ggccgacggc cgcgccgccg tcgtcgtcgg cacgcacgcg
      901 ctgttccagg aaagcgtgga gttcagacgg ctcgcgctcg tcgtcgtcga cgagcaacat
      961 cgcttcgggg tcgagcagcg gcgcaggctc aagcagaaag gggccgacga cgcgcgcgtg
     1021 ccgcaccagc tgatcatgac cgcgacgccg atcccgcgca cgctcgcgat gacggcgtac
     1081 gccgatctcg actgctccgt gatcggcgag ctgccgccgg gccgccagcc cgtgcacacg
     1141 gcggtcgtgc ccgagcagcg gcgcgccgag ctcgtccggc gcgtcgcggc gcattgcgcg
     1201 gccggccagc aggcttactg ggtctgcccg ctgatcgagg agtccgagct cgtcgactcg
     1261 cgcgcggcga gcgcgcttca cgccgagctc gccgaagcgc tgccgaacgc gcgcgtcggc
     1321 ttgattcacg ggcgcatgaa gtccgccgag aaagacgccg tgatgcgcgc attcaaggct
     1381 cgcgagctcg acctgctcgt cgcgacgacg gtcatcgagg tcggcgtcga cgtgccgaat
     1441 gcgacgctga tggtcgtcga gaacgccgag cgcatgggac tcgcgcagct gcaccagctt
     1501 cgcggccgag tcggccgcgg cgcgaaggcc tcgacgtgca tgctgctcta tcgcgcgccg
     1561 ctctcggatc tcgcgcggca gcgcctcggc gtgctgcgcg agacgaacga cggcttcgcg
     1621 gtcgcgcaga aggacctcga gctgcgcggt ccgggcgagg tgctgggtac gcgccaaacg
     1681 ggcgtcatgc agctgcgcat cgccgatctt ttgcgcgacg cggatctgtt gccgcaggtc
     1741 atcgagatct cggaccagct gctcgccacg gcgccggaag ccgccgacgc gctgattcgg
     1801 cgctggattc gcggcgaagc agagtatgca aaggtctgaa agcgcgcgtt gccggccggg
     1861 cctcgcgtag aatcgccgcg atgcgcttgg gtccggaatc cgaacgttga gcactcggtc
     1921 gacacgcgtg actgcggcgg gaatggcttg gggccatgcc caggcgctgc tcgcccacta
     1981 cgcgcgctac tggcggctga tgcgcctgca ccggccgatc gggatctggc tgcttctctg
     2041 gcccacgctc tgggcgcttt ggatcgcgtc ggccgggcgg cccgatccga aggtgttcgt
     2101 cttcctcgtg ctcggcacga tcgtcgtccg ctcggccggg tgtgtcatca acgacttcgt
     2161 cgaccgcaag atcgacccgc acgtcgcgcg gacggccgat cggccgctcg cgacgggcga
     2221 ggtcgcgccc gtggaggcgc tgctgctgtt cggcgcgctg atgctgatcg cgctcgggct
     2281 cgtgctgaac ttgaacaggt tgacgctgac gttcgcggcc gccggagcgt tcctgacgat
     2341 cgtctatccg ttcacgaagc gcttcctctc gacgccgcaa ttcgtgctcg gcgtcgcgtt
     2401 ctcgtgggga gtgccgatgg cgttcgccgc gacggccggc gacgtgccgc gcgtcggctg
     2461 gctgctgttt ctctcgaccg tgatctgggt cgtcgtctac gacacgcagt acgcgatgac
     2521 ggatcggccc gacgacatca agctcggcgt gcgctcgacg gcgatcttgt tcggcgagct
     2581 cgaccgaacg ttcatcgtgg gcctgcaggc gctgtttctc gcgagcctcg tgctcgtcgg
     2641 gcgcagcgcg gcgatgggcc cgtggtacta cggcggcctg ggcgtggcct gcgtgttctg
     2701 cttgtaccag gcgtatctga tcaaggagcg cgacatcgtt cagtcgttcc gcgccttctt
     2761 gaacaacgct tggctcggcg gcgccatctt cgccggcatt gtgctcgact acacgttccg
     2821 tccttgagcg ggctccggag ttcgcgcgac ggcgagcacg acgcagcgct ccgcgcccgc
     2881 cgccttgagc tcgacggcga gcgcgttgac cgtggcgccc gtcgtgacga cgtcgtcgac
     2941 gatggcgatg tgccgtcccc gtagatcgca cgacacgcgg aatgcgcgcg ccacgctgcg
     3001 ccgccgctcg agcatgccct gcccggcctg ctttggcgtc gcgaccggcc gcgcgatgcc
     3061 gcgcatcatg gccggcaggc cgagcgctcg cgcgagcacg cgcgcgatct cttgcgcctg
     3121 gttgtagccg cgctcgcgca agcgcgagcc gtgcagaggc acggccgcga gcgcgtcgac
     3181 gccgtcctgc tccgcgagcg aaggcatgag cagcaacgca aacgcacgcc ccatggcgcg
     3241 cgcgccgcgg tacttcatgg cttgcacgta gtgatcgagc ggcggccgat acgagaacgg
     3301 cgcgatgacg gcgtcgacgt cccacgccgt ctccttgcgc gggcaatgcg cgacgggctc
     3361 ggctaggccg cagcggcggc aggcgcgcgc gatgcgcggc agctgcgcgg cgcagacgcc
     3421 gcaaaaaccg ccgcgggagg cgaggccgca cgcggggcag ctctccgtga acaagcggtc
     3481 gaaaatcccg taaaccacga caactccaag cggttgacag tgcactccgc cgtttgtgat
     3541 gctccagcgt cgcgcgcgaa gcgcttagcg agccatcggc gaatgtcggc cattccacga
     3601 gataccgcgc tcgacccgcc cgtcgcggcc gggccgcgcg ccgattggac gagacccgag
     3661 gtcgaggcgc tgttcgagct gccgttcaac gatctcttgt tcaaggcgca gtcggtgcat
     3721 cgacagactt tcgacccgaa cgccgtgcag gtctcgacgc tgctgtcgat caagaccggc
     3781 gcgtgtcccg aggactgcgc gtactgcccg caaagcattc gcttcgagac cggcgtcgac
     3841 acgcacgacc tgatgccgct cgagcaggtg cgcgccgcgg cgcagcgcgc caaggacgcc
     3901 ggggcgacgc gcttctgcat gggcgcctcg taccgcgggc cgaaggattc gcagctcgag
     3961 ccgatcctcg agatggtcaa ggccgtcaag tccgtcgggc tcgagacctg cgcgacgctc
     4021 gggctgctgc ggccgggcca agccgagcgg ctcgccgaag cggggctcga ctactacaac
     4081 cacaacctgg actcgtcggc cgagttctac ggcaagatca tcagcactcg aacctatgcc
     4141 gatcggctcg agacgctcga gcgcgtgcgc gcggcgggcg tcaaggtctg ctgtggcggg
     4201 atactcggca tgggcgagac gcgagccgac cgcgtcggct tgctgcacac gctggcgacg
     4261 ctcgttccgc agcccgaaag cgtgccgatc aacgagctgg tcccgatccc gggcacgccg
     4321 ctcgcgtccg cagagccgct cgacccgctc gagttcgtgc gttgcatcgc cgtggcgcgc
     4381 atcctgatgc cgcgctcgca cgtgcggctc tcggcgggcc gcagcgactt cagcgacgag
     4441 ctgcaggcgc tgtgcttttt cgcgggcgcg aactcgatct tctacggcga gaagctgctg
     4501 acgacgggca atcccgatac ggcggccgat cgacgcctgt tcgaccggct cggattggca
     4561 cccgagggca cgggcacgga tgcccgcggt cgagcggcgc agaacgcggt tcccgagggg
     4621 ctcgcggcga gctgagcgga agcgccgtgc gcaatccatg tctctgagcc tcgacgacgc
     4681 gctgcccgag gcgcgcgccg cgcggcgcgc gttcgatcgc gcgcgcgcct tcgagtcggc
     4741 atccttcatc cacgacgagg cgcgcgcgcg cctcggcgcg cggctcgacc
//
LOCUS       DASSNN010000595         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1233318, whole genome shotgun sequence.
ACCESSION   DASSNN010000595 DASSNN010000000
VERSION     DASSNN010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233318"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFL84_11000"
     CDS             <1..544
                     /locus_tag="VFL84_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7609195.1"
                     /translation="TDVTWPWNSETVEPLPKVGLPNKTFYEETELTVGGRTVQCGHLR
                     DCPHTDGDMYVYFREDNVLAVGDAVYGVGWPEIDWWTGGWIGGLVGGLDMLFYVANDD
                     TRIVPARGPVLTRAELRKQYDMYSVIWERLVKTLYSGGGPKEALAAKPTQEFDAIMGP
                     SDAFVERAFQSLWAYLSPDA"
     gene            570..>2056
                     /locus_tag="VFL84_11005"
     CDS             570..>2056
                     /locus_tag="VFL84_11005"
                     /inference="COORDINATES: protein motif:HMM:NF019255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HET7609196.1"
                     /translation="MKRIRSTKAFSTALRLAGALAASTVLAACNPFGTASVADRAAEQ
                     WPLVNKYCTDCHNAAELAGGLDFEKINPTNVAQHAEKLEMAVRKLRSHAMPPPKEPRP
                     DERRLQSFIVWLQDALDEAAAANHGSQPVVPHRLNRKEYANAIRDLLALEIDPVEFLP
                     QDEEVKHFDNIASGLQVSPSFVEQYVGAARQIAVRAVGRPDARTGGQTYFAPPGRQQA
                     HVKGLPLGTRGGFVVTHLFPSDGEYEVNIADMFSHIWGNDSEFENTVVVTLDGKTVYE
                     TTLGGEEDTRAYDQVQNGVMEKVNGRLKDIRFAATAGPHKVGVTFKRRTFAESDDQLQ
                     AFVPGGGQDRVFRVASFEVSGPYNPTGLSETPSRDKIFSCHPSRGDDEELCAERILTE
                     LATRAYRRPLAEEEVSDLLAYYRDGVATNGFEEGIRSGLTGILASPYFLYRIETPPAN
                     VKPGDVYALDDVTLASKLSFFLWNTIPDDELRELAVKGQLHDDKVL"
BASE COUNT          370 a          691 c          681 g          314 t
ORIGIN      
        1 gaccgacgtg acctggccgt ggaacagcga gaccgtcgag ccgctgccca aggtcgggct
       61 gccgaacaaa acgttctatg aggaaacgga gcttacggtc gggggtcgaa ccgttcagtg
      121 cgggcaccta cgcgactgtc cgcacacgga cggcgacatg tatgtctact tccgcgagga
      181 caacgtgctc gcggtcggcg acgccgtcta cggggtgggt tggcccgaga tcgactggtg
      241 gacgggcggc tggatcggcg gcctcgtcgg cggtctcgac atgctgttct atgtcgcgaa
      301 cgacgacacc cgcatcgtgc cggcgcgggg ccccgtgctg acgcgtgccg agctgcgcaa
      361 gcagtacgat atgtacagcg tgatctggga gcggctcgtc aagacgctct acagcggggg
      421 cggccccaaa gaggcgctcg ccgcgaagcc cacgcaggag ttcgatgcga taatgggccc
      481 gtcggacgcg ttcgtcgaac gcgcgttcca gagtttgtgg gcgtatctgt cgcccgatgc
      541 ctagcccgat gcggagcctg gatagaaacg tgaaacggat tcgatcgacg aaagcattct
      601 ccactgcgct gcggctcgcc ggcgcgctcg cggcttccac cgtgctcgcc gcctgcaatc
      661 cgttcggcac ggcgtccgtc gccgatcgcg ccgccgagca gtggccgctc gtcaataagt
      721 actgcaccga ctgccacaac gccgccgagc tcgcgggcgg gctcgacttc gagaagatca
      781 accccacgaa cgtcgcgcaa catgccgaga agctcgagat ggccgtgcgc aagctgcgca
      841 gccacgcgat gccgccgccg aaagagccgc ggcccgacga gcggcggctc caatcgttca
      901 tcgtatggct ccaggacgcg ctcgacgagg ccgcggccgc caatcacggc tcgcaacccg
      961 tcgtgccgca tcggctgaat cgcaaggagt acgcgaacgc gatccgcgac ttgctcgcgc
     1021 tcgagatcga tcccgtcgag ttcttgccgc aggacgaaga ggtcaagcac ttcgacaaca
     1081 tcgcgagcgg gctgcaagtg tcgccgtcgt tcgtcgagca gtacgtcggc gcggcgcgcc
     1141 agatcgccgt gcgcgccgtc ggccggcccg acgcgcgtac gggcggtcag acttacttcg
     1201 cgccgccggg ccggcagcag gcgcacgtga agggcttgcc gctcggcacg cgcggcggct
     1261 tcgtcgtcac gcacttgttc ccgtccgacg gcgagtacga agtcaacatc gccgacatgt
     1321 tcagccacat ctggggcaac gactcggagt tcgagaacac cgtcgtcgtg acgctggacg
     1381 gcaagaccgt ctacgagacg acgctcggcg gcgaagagga cacgcgcgcc tacgaccaag
     1441 tgcaaaacgg cgtcatggag aaggtcaacg gccgcctcaa ggacatccgt ttcgccgcga
     1501 cggccggacc acacaaggtc ggcgtgacgt tcaagcgccg cacgttcgcg gagtccgacg
     1561 atcagctcca ggcgttcgtg ccgggcggcg gccaggaccg cgtgttccgc gtggcgtcgt
     1621 tcgaggtcag cggtccctac aatccgacgg gcctaagcga gactccgagc cgcgacaaga
     1681 ttttttcgtg ccatccgtcg cgcggcgacg acgaggagct gtgcgccgag cgcatcctga
     1741 ccgagctcgc gacgcgcgcg tatcgccggc cgctcgccga ggaagaggtc agcgatctgc
     1801 tcgcctacta ccgcgacggt gtcgcgacca acggcttcga ggaaggcatc cgcagcgggc
     1861 tcaccggcat tctcgcgagc ccgtatttcc tctatcgcat cgagacgccg cccgcgaacg
     1921 tcaagcccgg cgacgtctac gcgctcgacg acgtcacgct cgcgtcgaag ctgtcgttct
     1981 tcctctggaa cacgatcccc gacgacgagc tgcgcgagct cgccgtgaaa ggccagctgc
     2041 acgacgacaa ggtgct
//
LOCUS       DASSNN010000596         6855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_479249, whole genome shotgun sequence.
ACCESSION   DASSNN010000596 DASSNN010000000
VERSION     DASSNN010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479249"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFL84_11010"
     CDS             <1..522
                     /locus_tag="VFL84_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7609197.1"
                     /translation="PHVIAGQGTLALELARFARARHVAIDAFYAPCSGGGLVGGCALA
                     LRAEWPSCAVYAVEPKGFEDHAASLEAGERRTVAPGATTLCDGLRAPTPGAITFAINK
                     RELAGARAVDDAQVREAMALAVEHLKVVVEPSGAAALAAVLVEPRPIGACVAVVLSGG
                     NVDAELLAEVLRT"
     gene            597..4004
                     /locus_tag="VFL84_11015"
     CDS             597..4004
                     /locus_tag="VFL84_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HET7609198.1"
                     /translation="MQLRQIARLDDWYRFDETGPLFITGLQALVRLTLAQSHRDRLAG
                     RNTAGFVSGYRGSPLGGLDRELWRAAPYLDAAQIRFQPGVNEDLAATSIWGTQQANLF
                     EGARHDGVFGMWYGKGPGVDRSGDALKHGNAAGTSRYGGVVAVAGDDHTCKSSSLPHQ
                     SEYAFIDASMPVLNPANVAEIVELGLYGYALSRFSGCWVGLKVTQETADATQSFALSP
                     QALSIVAPDFAMPPGGLNIRWPDPPNDQELRLHRYKLPAALAFARANALDRTIIDSRY
                     ARLGLVTTGKAHLDVLQALEDLGLDAARAGEVGIKLFKVGMSWPLEPEAIRQFAEGLE
                     EIVVVEEKRGVVESQLKEQLYNWQAKRRPLIVGKQDERGEWLLPGTGELTPALIARVL
                     AKRMRRFHSSTEIEERVRFLEQQDRRLAVVDADVQRLPHFCSGCPHNTSTRVPEGSRA
                     VGGIGCHYMAAWMDRDTVTYTQMGGEGATWIGQAPFTETRHVFQNLGDGTYAHSGTLA
                     IRAAVAAQVNMTYKILFNDAVAMTGGQPVEGHLTVAGVAQQLVGEGVRPILVVTDHPE
                     KYAGANGLPPSVAVHHRRELDRLQRELREVRGVSALIYDQTCAAELHRKRKRGTVPTP
                     DRRVIINELVCEGCGDCSVQSNCLSVMALETELGRKRRINQSSCNQDFSCLNGFCPSF
                     VTLRGAQRNPPRPLAAAALPSLPEPKQAALDGVFNALIAGVGGTGVVTASGLLGLAAH
                     LEGKSVVQLDQTGLAQKYGGVLSHVRIGADLIVAAGKEPLSMLSPDRSAVLVNTHEEM
                     PSDFIRDRDFTFPGKRLVASLRAAGRSGAVATLDATRLAAALLGDSIGANVLMLGFAF
                     QRGLLPVSGAALYRALELYGRNVEENKLAFDWGRFAAESLAEVERLAMEGAQSISTSL
                     TDAIARREEYLVGYQNRAYAQRYRAKLARIAAVEQRVRPGSSELQDAVARNYFSVLAY
                     KDEYEVARLHTETGFVESIKKNFGERARMSFHFSPPLLARKDPATGRPKKYELGPWVL
                     PILRVLAKLKWLRGTKLDPFARSADRRLERELLARYESLLDRIADELDDSRFALAVEL
                     AKLPQQVRGFGPLKAAAAQRARESEQSLWQQWVAPAARVVRARASAA"
     gene            complement(4039..4383)
                     /locus_tag="VFL84_11020"
     CDS             complement(4039..4383)
                     /locus_tag="VFL84_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020584910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HET7609199.1"
                     /translation="MDQYARIRDQLIAKQTELARRLERLKENLTGGRSADSQEQAQEL
                     ENAEVVDALGNEARVEISKIARALDQIKNNTYGICIDCGETIPMARLEAYPFADRCIR
                     CATAAESSRRPR"
     gene            4518..5219
                     /locus_tag="VFL84_11025"
     CDS             4518..5219
                     /locus_tag="VFL84_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET7609200.1"
                     /translation="MRLLAGTSGFSYKEWVGAFYPDKLAAKDMLAHYATRLPIVEINN
                     TFYRMPNAASIQSWRSQVPDSFRFAIKVPRRISHIKRLRDCGEDLAFLLRAVADLSPC
                     LGSLLVQLPPYARCDEAALASFLSRVPEGTRAAFEFRNDSWHVPAVFKLLESRNAALV
                     QSESDETFEPLPRTADWAYLRLRRVDYTTEDLHRWLERIDASGVTEAQVFFKHEDGAT
                     GPKLAAQLLAMADRR"
     gene            5408..>6855
                     /locus_tag="VFL84_11030"
     CDS             5408..>6855
                     /locus_tag="VFL84_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7609201.1"
                     /translation="MSPSKPVARMAASRRLPKRAAGSRIADERLQSVIDLAVDFYWEQ
                     DAHYRFTVYRPSGDADAELLGLVGKRSAEFFHEIAETDGRESLAATLARRAVFRDVLH
                     RLGSEPASARYFHFSGQPVFDQRNKFKGYRGIARDVSAQVRAERLVQLESALAQMLAG
                     ANDLATGLTAVIRAMCEALTWTAGNFWTVDAQRDTLRHEVGWNSLGEERRSMLSSGDR
                     LPHWLTHGPVWIGDTTRNPRTSRLEPAEADGWSTGLLIPVKAGGATIGVLDFYAPRVA
                     APEPQFLKLLRNAGAEIGRFYQRAHALDRLRESEERFSSTMALAAIGIAHVEDDGRFR
                     YVNPQLCAMLGYTEQELLAMTVKDVSHLGDANLTDDLRDQLRSGAIQSFKMEKRYLRK
                     DGSPIWVGLTIACKRDRNGECLYDISVVEDISSRKRAEERIQYLATHDGLTGLPNRLM
                     FMQLLGLSIETARRYGRKLAVLFIDLDRFKII"
BASE COUNT         1092 a         2305 c         2335 g         1123 t
ORIGIN      
        1 ccgcacgtca tcgcggggca gggcacgctc gcgctcgagc tcgcgcgctt cgcgcgggcc
       61 cggcatgtcg ctatcgacgc attctatgcg ccgtgcagcg gcggcgggct cgtcggaggc
      121 tgcgcgctcg cgctgagggc cgaatggccg agctgcgccg tctacgccgt cgagccgaaa
      181 gggttcgagg atcacgcggc ttcgctcgaa gccggcgagc ggcgcacggt cgcgcccgga
      241 gcgacgacat tgtgcgacgg gctccgcgcg ccgacgccgg gcgcgatcac gttcgcgatc
      301 aacaagcgcg agctcgcggg cgcgcgtgcc gtcgacgacg cgcaggtccg cgaggccatg
      361 gcgctcgcgg tggagcatct caaggtcgtc gtcgagccgt cgggggctgc ggcgcttgcg
      421 gccgtgctcg tcgagccgcg accgatcggc gcgtgtgtcg ccgtcgtact ctccgggggc
      481 aacgtcgacg ccgagctgct cgccgaggtg ctgcgcacgt gatgaatcaa gccgccgccc
      541 gataattggc ccataatgcc gggcgccgac ctgcgcatcg gggaacagga gaggcattgc
      601 agctcaggca gattgcacgg ctggacgatt ggtatcggtt cgacgaaacc ggtccgctgt
      661 tcatcacggg tctgcaagcg ctcgtgcgcc tcacgctcgc gcaaagccac cgcgaccggc
      721 tcgccggccg gaacacggcc ggcttcgtct cgggctatcg cggctcgccg ctcggcggcc
      781 ttgaccgcga gctgtggcgc gcggcgccgt acctcgacgc cgcgcagatc cgctttcagc
      841 ccggcgtgaa cgaggacctc gcggccacct cgatctgggg cacgcagcag gccaacctgt
      901 tcgagggtgc ccgccacgac ggcgtgttcg gcatgtggta cggcaagggg cccggcgtcg
      961 accgctccgg cgacgcgctc aagcacggca acgcggccgg cacgtcgcgc tacggcggcg
     1021 tcgtcgcggt cgcgggcgac gatcacacgt gcaagtcgtc gtcgctgccc catcaaagcg
     1081 agtacgcgtt catcgacgcg tcgatgcccg tgctgaaccc ggccaacgtc gccgagatcg
     1141 tcgagctcgg cctgtacggc tatgcgctgt cgcgcttcag cggttgttgg gtgggactga
     1201 aagtcacgca ggagacagcc gacgcgacgc agagcttcgc gttgtcgccg caagcgctgt
     1261 cgatcgtggc gccggatttc gcaatgccgc cgggcggctt gaacattcgt tggccggatc
     1321 cgccgaacga tcaagagctg cgcttgcatc gctacaagct gccggccgcg ctcgcgttcg
     1381 cgcgcgccaa cgcgctcgac cgcacgatca tcgacagccg ctatgcgcgc ctcgggctcg
     1441 tcacgacggg caaagcgcac ctcgacgtgc tacaggcgct cgaggacctc gggctcgacg
     1501 ccgcgcgcgc tggcgaagtc ggcatcaagc tgttcaaggt cggcatgagc tggccgctcg
     1561 agcccgaggc gatccgccaa ttcgccgaag gcctcgagga gatcgtcgtc gtagaggaga
     1621 agcgcggcgt cgtcgagagc cagctcaagg agcagctcta caactggcaa gcgaagcgcc
     1681 ggccgctgat cgtcggcaag caggacgagc gcggcgagtg gctattgccg ggaacgggcg
     1741 agctcacgcc cgcgctcatc gcgcgcgtgc tcgcgaagcg catgcggcgc tttcattcga
     1801 gcaccgagat cgaggagcgc gtgcgcttcc tcgagcagca ggaccgccgc ctcgcggtgg
     1861 tcgacgcgga cgtgcagcgc ttgccgcact tctgctcggg ttgcccgcac aacacctcga
     1921 cgcgtgtgcc cgagggcagc cgagccgtcg gcggcatcgg ctgccactac atggcggcgt
     1981 ggatggaccg cgacaccgtc acctacacgc agatgggcgg cgagggcgcc acgtggatcg
     2041 ggcaggcccc gttcaccgag acgcggcacg tgttccagaa cttgggcgac ggcacgtacg
     2101 cgcattccgg cacgctcgcg atccgcgcag ccgtcgcggc ccaagtcaac atgacgtaca
     2161 agatcctgtt caacgatgcc gtcgcgatga cgggtggcca gcccgtcgaa gggcacctca
     2221 ccgtcgcggg cgtcgcgcag cagctcgtcg gcgaaggcgt gcggccgatt ctcgtcgtca
     2281 ccgaccaccc ggagaagtac gccggcgcga acggtttgcc gccgtccgtg gccgtgcatc
     2341 atcgccgcga gctcgaccgc ttgcagcgcg agctgcgcga ggtgcgcggc gtgtcggcgc
     2401 tgatctacga ccagacttgc gcggccgagc tgcaccgcaa gcgcaagcgc ggcacggtgc
     2461 cgacgccgga tcggcgcgtc atcatcaatg agctcgtctg cgaaggctgc ggcgactgca
     2521 gcgtgcagtc gaattgcctg tccgtgatgg cgctcgagac cgagctcgga cgcaagcggc
     2581 gcatcaatca gagctcgtgc aaccaggact tcagctgctt gaacgggttt tgcccgagct
     2641 tcgtgacgct gcgcggcgcg cagcgtaatc cgccgcggcc gctcgccgcg gcggcgctgc
     2701 cgagcttgcc ggagcccaag caagccgcgc tcgacggcgt cttcaacgca ttgatcgcgg
     2761 gcgtcggcgg caccggcgtc gtgacggcga gcggcttgct cggtctcgcc gcgcatctcg
     2821 aaggcaaatc ggtcgtgcag ctcgaccaga cggggctcgc gcagaagtac gggggcgtgc
     2881 tctcgcacgt gcgcatcggc gccgacctga tcgtcgccgc gggcaaagag ccgttgtcga
     2941 tgctgtcgcc ggaccgctcc gccgtcctcg tcaacacgca cgaggagatg ccgtcggact
     3001 tcattcgcga ccgcgacttc acgttcccgg ggaagcggct cgtcgcgtcg ctgcgcgcgg
     3061 ccgggcgctc gggcgccgtc gcaacgctcg atgcgacgcg cctcgccgcg gcgctgctcg
     3121 gcgacagcat cggcgcgaac gtgctgatgc tcgggttcgc gttccaacgc gggctgctgc
     3181 cggtatccgg cgcggcgcta tatcgcgcgc tcgagctata cggccgcaac gtcgaggaaa
     3241 acaagctcgc gttcgactgg ggcaggttcg ccgcggagag cctcgccgaa gtcgagcggc
     3301 tcgcgatgga aggcgcgcaa tcgatctcga cgtcgctgac cgacgcgatc gcgcggcgcg
     3361 aggagtacct cgtcggctat cagaatcgcg cctacgcgca gcgctaccgc gcgaagctcg
     3421 cccgcatcgc cgcggtcgag cagcgtgtgc ggcccggcag cagcgagttg caggatgccg
     3481 tcgcgcgtaa ttacttcagc gtgctcgcct acaaggacga gtacgaggtt gcgcgcctgc
     3541 acaccgagac cggtttcgtc gagagcatca agaagaactt cggcgaacgc gcgcgcatga
     3601 gctttcactt ctctccgccg ctcctcgcgc gcaaagaccc tgcgactggg cggccgaaga
     3661 agtacgagct cggcccctgg gtgctgccga tcctgcgcgt gctcgcaaag ctcaaatggc
     3721 tgcgcggcac caagctcgat ccgttcgctc ggagcgcgga ccggcgtctc gagcgcgagc
     3781 ttctcgcgcg ttacgagtcg ctgctcgatc gcatcgcgga cgagctcgac gattcgcgtt
     3841 tcgcgcttgc ggtcgagctc gcgaagctgc cgcagcaggt gcgcggtttc ggaccgctca
     3901 aggccgcggc cgcgcagcgt gcgcgcgagt cggagcagag cctgtggcag cagtgggtgg
     3961 ccccggccgc tcgagtcgtg cgggcgcgcg caagcgccgc ctaatcgttc gctgaaggta
     4021 gtaagcgagc gctcgagctc agcgaggacg gcgcgaggac tccgccgccg tcgcgcagcg
     4081 aatgcagcgg tcggcgaacg ggtacgcctc gagccgcgcc atcgggatcg tctcgccgca
     4141 gtcgatgcag atgccgtagg tgttgttctt gatctggtcg agcgccctcg cgatcttgct
     4201 gatctcgacg cgcgcctcgt tgccgagcgc gtcgacgacc tcggcattct cgagctcctg
     4261 cgcctgctcc tgcgagtcgg cgcttcgccc accggtgagg ttttccttga gacgctccaa
     4321 gcggcgcgcg agctcggtct gtttcgcgat gagctgatct ctgatgcggg cgtactgatc
     4381 cattctcgat tgcgctcaag acgccagttg caagctatcc acgggcgcct ctagcatcga
     4441 ggcgcagagg ggacgaatgg tcttgttcgt cccaagcata gctcgtctcg caccgctgcg
     4501 ggaaaaaagg tggctcgatg cgtttgctcg ccggtaccag cggtttctcc tacaaagaat
     4561 gggtcggcgc gttctatccc gacaagctcg cggccaagga catgctcgcg cactatgcga
     4621 cgcggttgcc gatcgtcgag atcaacaata cgttctatcg aatgccgaac gcggcttcga
     4681 tccaatcgtg gcgttcccaa gtacctgatt cgtttcgctt tgcaatcaag gtgccgcggc
     4741 gcatctcgca catcaagcgg ctgcgcgatt gcggcgagga cctcgcgttc ctccttcgtg
     4801 cggtcgccga cttgtccccg tgtctcggct cgttgctcgt gcagctgccg ccgtacgcgc
     4861 gctgcgacga agcggcgctc gcgagtttcc tgagccgcgt gccggagggc acgcgcgccg
     4921 cgttcgagtt tcgcaacgat tcgtggcacg tgccggcggt gttcaagctg ctcgagtcgc
     4981 gcaatgcggc gctcgtgcag agcgagagcg acgagacgtt cgagccgctg ccccggaccg
     5041 ccgactgggc gtatctgcgg ctgcgccgcg tcgactacac gaccgaggat ctgcaccgct
     5101 ggctcgagcg aatcgacgcg tccggcgtca cggaggcgca ggtcttcttc aagcacgagg
     5161 acggtgccac cggcccgaag cttgccgcgc aattgctcgc gatggccgac cggcgctagg
     5221 gcttcggatt cgcacgaaaa gcgccaatct gtcgcagacg ttaattgggt cgcactcgtc
     5281 gagccgcgag tgagaggaat ctcgcaatcg gggcacccgc cggcgtttta ttctcattaa
     5341 tgcgacgacc ggggaccgcg tacaccccga cgttctcgcg ttatccaaag gaccggttgg
     5401 cgagcacatg agccccagta aacccgtcgc gcgcatggcg gcgtcgcgac ggttgcccaa
     5461 gcgcgccgcc ggctcgcgaa ttgccgacga gcgattgcaa tccgtgatcg atctcgcggt
     5521 cgacttctat tgggagcagg acgcgcacta ccggttcacg gtgtaccggc cgagcggcga
     5581 cgccgatgcc gagctcttgg ggctcgtcgg caagaggagc gcggaattct tccacgagat
     5641 cgcggagacg gacggccgag agtcgctcgc cgcgacgctc gcgcggcgcg ccgtgtttcg
     5701 cgacgtgctg catcggctcg gctccgagcc ggcgagcgcc cgctacttcc acttcagcgg
     5761 ccaaccggtc ttcgaccagc gcaacaagtt caagggttat cgcggcatcg cgcgcgacgt
     5821 cagcgcgcag gttcgggcgg agcgtctcgt tcagctcgag agcgcgcttg cgcagatgct
     5881 cgcgggcgcc aacgacctcg cgacgggttt gacggcggtc attcgcgcga tgtgcgaagc
     5941 gctgacctgg actgccggca acttctggac cgtcgatgcg caacgtgaca cgttgcgcca
     6001 tgaggtcggt tggaactcgc tgggcgagga gcgtcgctcg atgctctcat cgggcgaccg
     6061 gctgccgcac tggttgactc acggccctgt ctggatcggc gatacgacac gcaacccgcg
     6121 cacgtcgcgc ctcgagccgg ccgaggccga cggttggagc acggggctct tgatcccggt
     6181 gaaggccggc ggcgccacga tcggcgtcct cgacttctac gcgccgcgag tcgcggcgcc
     6241 cgagccgcag ttcctaaagc tgctgcgcaa tgcaggagcg gagatcggcc gcttctatca
     6301 gcgcgcgcac gcgctggaca ggctgcgcga gagcgaagaa cgcttctcgt cgacgatggc
     6361 gctggccgcg atcggcattg cgcacgtcga agacgacgga cgcttccgct acgtcaaccc
     6421 gcagctctgc gcgatgctcg gctataccga gcaagagctg ctcgcgatga ccgtcaagga
     6481 cgtctcgcac ctcggcgacg cgaacctcac cgacgacctg cgcgaccagt tgcgctccgg
     6541 tgcgatccag tcgttcaaga tggagaagcg ctacttgcgc aaggacgggt cgccgatttg
     6601 ggtcggtctc acgatcgcct gcaagcgcga tcgcaacggc gagtgcctct acgacatctc
     6661 ggtcgtcgag gacatctcct cgcgcaagcg cgccgaagag cgcatccaat atctcgcgac
     6721 ccacgacgga ctgaccggct tgccgaatcg tctgatgttt atgcagctcc tcggcttgtc
     6781 gatcgagacg gctcggcgct acggccgcaa gctcgcggtg ctgttcatcg acctcgaccg
     6841 tttcaagatc atcaa
//
LOCUS       DASSNN010000597         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1114731, whole genome shotgun sequence.
ACCESSION   DASSNN010000597 DASSNN010000000
VERSION     DASSNN010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114731"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VFL84_11035"
     CDS             complement(<1..948)
                     /locus_tag="VFL84_11035"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006984723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET7609202.1"
                     /translation="MKQKSKTPVRVAITGAAGQIGYQLAFRIASGQLFGEDQPIILQL
                     LEITPALGALSGVAMELDDCAFETLHGIVVTDKAEVAFKDINYGLLVGARPRGPGMER
                     KDLLQANAQIFSAQGKALDAVADRRVKILVVGNPANTNALIARENAKGLDPRNFTAMM
                     RLDHNRAKSQLAAKTGSHVGDIKGVIVWGNHSATQFPDLTHATVAGKPALSRVSPSWY
                     RDEFIPTVQQRGAAIIKARGASSAASAASAAIDHVRDWALGTPGDDWVSMAVSSDGSY
                     GIEEGVIYSYPVRCVRGDYQIVQGLSVDDFARAKMAATEA"
     gene            complement(1058..2080)
                     /gene="pspF"
                     /locus_tag="VFL84_11040"
     CDS             complement(1058..2080)
                     /gene="pspF"
                     /locus_tag="VFL84_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HET7609203.1"
                     /translation="MIGEAPAFLAMLEHVSRAAKLVKPVLVIGERGTGKELIASRLHY
                     LSPRWEQPLIKVNCAALTETLLESELFGHQAGAFTGATRTHIGRFEQANGGTLVLDEL
                     GTIPPRMQEKILRVIEYGEFQRVGGSDTIRTDVRIVGATNEHLPRLAELGRFRADLLD
                     RLAFDVIHVPPLRARPEDINTLAYHFAVNVTKELRRPFFPGFTSDAKAALLGYAWPGN
                     VRELKNAVERSVYRSETPDQPINEILFDPFASPFSPAPPREPDEEDEQPESSSLTTAP
                     ASVGVPADFRAAVADFERQVLRRALERAQFKQTVAAKILGLSYHQFRSLLRKHGLAKQ
                     GLDSAD"
     gene            2310..2990
                     /gene="pspA"
                     /locus_tag="VFL84_11045"
     CDS             2310..2990
                     /gene="pspA"
                     /locus_tag="VFL84_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019025761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HET7609204.1"
                     /translation="MGIFSRFTDIVNSNINSILDKAEDPEKLVRLMIQEMEDTLVEVR
                     STAARAIADRKEITRTLTNLEAEVRDWQTKAELALDKNREDLAKAALAEKARAANAAE
                     ALRAQHQALGDGLDKLNDDVVKLEEKLVDAKTRQQAILSRQRTASRRLEARKRLHSAR
                     IDDALIRFDQFERRIDDIEAHVESYDLGGGKGLKDQFSDLETEDAVAQELRELKSKRA
                     GQKRNEPA"
     gene            2994..3227
                     /gene="pspB"
                     /locus_tag="VFL84_11050"
     CDS             2994..3227
                     /gene="pspB"
                     /locus_tag="VFL84_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HET7609205.1"
                     /translation="MDSTTGVFVVTIVFMTVVLPFIVIMHYSTKWKATKGLSDDEHRM
                     LEDLWKESQAMQSRVNALETILDSQVPDWRRQQ"
BASE COUNT          596 a         1046 c         1086 g          556 t
ORIGIN      
        1 ggcttccgtg gccgccatct tcgcgcgcgc gaagtcgtcg accgagaggc cctgcacgat
       61 ctggtagtcg ccgcgcacgc agcgaaccgg gtaagagtaa atgacgcctt cctcgatgcc
      121 gtagctgccg tccgacgaca ccgccatgct gacccaatcg tcgccgggcg tgccgagcgc
      181 ccagtcgcgc acatgatcga tcgcggccga tgcggcggaa gcggccgacg acgcgccgcg
      241 cgctttgatg atcgccgcgc cgcgctgctg cacggtcggg atgaactcgt cgcggtacca
      301 gctcggcgag acacgcgaca gcgccggctt gccggcgacg gtcgcgtgag tgaggtccgg
      361 gaattgcgtc gccgaatggt taccccaaac gatgacgccc ttgatgtcgc cgacgtgcga
      421 gcctgtcttg gccgcgagct gcgacttcgc gcggttgtga tcgagccgca tcatcgcggt
      481 gaagttgcgc gggtcgagcc ccttcgcgtt ctcgcgcgcg atcagcgcgt tcgtgttggc
      541 cgggttgccg acgacgagga tcttgacgcg ccgatcggcg accgcatcga gcgccttgcc
      601 ctgagccgag aagatctgcg cgttcgcttg caagagatcc ttgcgctcca tgccgggccc
      661 gcgcggccgc gcgccgacga gcaggccgta gttgatgtcc ttgaacgcga cctcggcctt
      721 atccgtgacc acgatgccgt gcagcgtctc gaacgcgcag tcgtccagct ccatggcgac
      781 gccgctcaac gcgccgagcg ccggcgtgat ctcgagcagt tgcaggatga tcggttgatc
      841 ctctccgaac aattgcccgg acgcgatgcg aaatgccagc tggtaaccga tttgacccgc
      901 ggcgcccgtt atggccacgc gaacgggcgt cttgctcttc tgcttcatgg atgacgtttg
      961 cctcgacaac gatactgtcc aaaaccgaat gcgggccgga atattaccac gcgagttcca
     1021 accggcaggc gccgtcgccg cgcttcacgt atgcgcgtca gtcggcgctg tcgagcccct
     1081 gtttcgcgag gccgtgcttg cgcagcaggc tgcgaaattg gtggtagctc aggcccaaga
     1141 tctttgccgc gacggtttgc ttgaactgcg cgcgctcgag cgcgcgccgc agcacttgcc
     1201 gctcgaagtc ggcgacggcg gcgcgaaagt ccgccggcac accgacgctc gcgggcgccg
     1261 tcgtgagcga gctcgattcg ggttgctcgt cttcctcgtc gggctcccgc ggcggcgcgg
     1321 ggctgaacgg cgacgcgaac ggatcgaaca aaatttcgtt gatcggctga tccggagtct
     1381 ccgagcggta gacgctgcgc tcgaccgcgt tcttgagctc gcggacgttg ccgggccagg
     1441 cataaccgag cagcgccgct ttcgcgtcgg aagtgaagcc cggaaaaaac ggccgccgca
     1501 gctctttcgt gacgttgacg gcgaagtgat acgcgagcgt gttgatatcc tcgggccgcg
     1561 cccgcaacgg cggcacgtgg atgacgtcga acgcgagccg gtcgagcagg tcggcgcgga
     1621 agcggccgag ctcggcgagc cgcggcaagt gctcgttcgt tgcgccgacg atgcgcacgt
     1681 cggtgcgaat cgtgtcggag ccgccgacgc gctggaactc gccgtactcg atgacgcgca
     1741 ggatcttctc ctgcatgcgc ggcgggatcg tgccgagctc gtcgagcacg agcgtgccgc
     1801 cgttggcctg ctcgaaccgc ccgatgtgcg tgcgcgtggc gcccgtgaac gcgcccgctt
     1861 gatgaccgaa cagctccgac tcgagcagcg tctcggtcag cgcggcacag ttgaccttga
     1921 tcaacggctg ctcccagcgc ggcgagaggt aatgcagccg cgaggcgatg agctccttgc
     1981 ccgtcccgcg ctcgccgatg acgagcacgg gtttgacgag ctttgccgcg cgcgagacgt
     2041 gctcgagcat ggccaagaag gccggcgcct cgccgatcaa accggtagtc tcgggcgtcg
     2101 tggtcgtcgt tgcggcggca gcgctggcca agtggcacca actgttggtc gtttaggcga
     2161 aatcttagcc caacaccaaa cgcttaccca agcgtcgttg cgaggatggg agcgaaaatt
     2221 cgagtaggtt caagccttta aggccggagt gaccctctgg cacagtttct gctttgaggt
     2281 ccgcactcac aactagaggg cctcgtgcca tgggcatctt ctcgcgcttc accgacatcg
     2341 tgaactcgaa catcaactcg atcctcgaca aggccgagga tccggaaaaa ctcgtgcgtc
     2401 tcatgatcca ggagatggag gacacgctcg tcgaggttcg ctcgacggcg gcccgcgcca
     2461 tcgcggatcg caaggagatc acgcgcacgc tgacgaatct cgaagccgag gtgcgcgatt
     2521 ggcaaacgaa agccgagctc gcgctcgaca agaaccgcga ggatctcgcg aaggcagcgc
     2581 tcgccgagaa ggcgcgcgcc gccaatgccg ccgaggcgct gcgcgctcag catcaggcgc
     2641 tcggcgacgg cctcgacaag ctcaacgacg acgtcgtgaa gctcgaggaa aagctcgtcg
     2701 acgcgaagac gcgtcagcag gcgatcctat cccggcagcg cacggcgagc cggcggctcg
     2761 aggcgcggaa gcgcctgcat agcgcgcgca tcgacgatgc gttgattcgc ttcgaccagt
     2821 tcgagcggcg catcgacgac atcgaagcgc acgtcgagtc gtacgatctc ggcggcggca
     2881 aagggctcaa ggaccagttc tcggatctcg agaccgaaga tgccgtcgcg caggagctgc
     2941 gcgagctgaa gtcgaagcgt gccggtcaga agcgcaacga gcccgcttga tccatggatt
     3001 caacgacggg agtcttcgtc gtcacgatcg tcttcatgac ggtggtcctg cccttcatcg
     3061 tgatcatgca ctactcgacg aagtggaagg cgacgaaagg cttatcggat gacgagcaca
     3121 gaatgctcga ggatctctgg aaagagagcc aagcgatgca gagtcgcgtg aacgctctcg
     3181 agacgattct cgacagccaa gttcccgatt ggagaaggca gcagtgaacg atcctcttcg
     3241 cgagccgggc ttgatcaaca ggctgtaccg caatccgcgg cgcg
//
LOCUS       DASSNN010000598         5357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_102052, whole genome shotgun sequence.
ACCESSION   DASSNN010000598 DASSNN010000000
VERSION     DASSNN010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102052"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFL84_11055"
     CDS             <1..366
                     /locus_tag="VFL84_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609206.1"
                     /translation="GIRLAILAALESVYECFPHITLPQYLITLEVRKAERDGNPHTLA
                     SIVKKLKMPFSTASRVVWSLTEEGGDIGIIKYLNHPTDRRKKLLVINERNEPNEIPRA
                     ITRAMLDYYGEAVKQLRGM"
     gene            complement(451..882)
                     /locus_tag="VFL84_11060"
     CDS             complement(451..882)
                     /locus_tag="VFL84_11060"
                     /inference="COORDINATES: protein motif:HMM:NF008437.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HET7609207.1"
                     /translation="MNKSMLMGLVAGIGVATAGGVLGYQYLGERNADTAAAEPQAAAP
                     ARAAPATRTAAAPAQECWDEEVADTPAPKDEHRVAGTAIGAVVGGAIGKELGDGDSDL
                     ATAAGAAAGALLGRKAQGEFQENRTTTHTERRCGPAGSAPR"
     gene            complement(970..1347)
                     /locus_tag="VFL84_11065"
     CDS             complement(970..1347)
                     /locus_tag="VFL84_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET7609208.1"
                     /translation="MCAAVWNPCLACGACCAFFRASFHWSEADPAGRGLTPAGLTAKL
                     TPHRAVMRGTEKTPPRCVALRGTIGKNVHCAIHALRPSPCREFQASWVDGLHNERCDR
                     ARAAHGLPPLSLPGDSRKAPSAP"
     gene            complement(1347..4931)
                     /locus_tag="VFL84_11070"
     CDS             complement(1347..4931)
                     /locus_tag="VFL84_11070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HET7609209.1"
                     /translation="MRLVAELLRLSATDLANHLGCGHLSRLELAVAEGRARRPHRNDP
                     IVDLLMERGREHEAGYLKHLRAQKLGVVEIRTAPGADNFEATLAAMRDGADVIYQAPL
                     GDDRWYGRADFLRKVAGESELGAWSYEVTDAKLATETRAGTILQLCVYSTLLERLQGR
                     APQQAHVVAPHHKFVPESYRLDDYSAYYRLVKHRLETALEARDAITYPEPAQHCDVCN
                     WWKECNARRRADDHLCFVAGISRLQIKELRTRLDVETLERLGELKVVPKPTRGSRDAL
                     ERARDQAAIQLKARRLQTRQHEVLPLGREHGFFRLPAPSRGDLFLDLEGDRLALDGGR
                     EYLFGISDARGSYTPLWAANPAEEKRAFERVVDRILAAFEDDRAMHVYHFGAYEPTAF
                     KRLSGRYATRENELDVILRAELFVDLHTIVKHSLRASVETYSLKDLEQFYGLARTQDL
                     RVATASRRAIEWAIEMRADLGLDAAPRAAAKPQLELGLDAEPSENKFAEHIAIVERYN
                     RDDCVSAARLRDWLEELRVAAEREWGGELERPELRSGEASDSVAETAEETLKVMAALL
                     EGVPVDAAERSEGEQARWLMAQLLEWHRREEKAAWWEYFRLLELPLEDYEDERSALAG
                     LTFVETVGGSPRKPIHRYAFPPQEHDVRRGDDVCVREINQPIGKIDALDVAGHTVDIE
                     QPSRHADTRPERVFVRRMVSPKPKPAVLLEIGRFIAASGADADGPHRAARDLLLRRPP
                     RLVSGSRGLLPAERQASTAPGRVIAFAAARSAVERQRAAAATPRAIEVDAEVKEAIRL
                     GFELDHGVLPIQGPPGTGKTFTGSHMILELIKAKKKVGVTAVGHETIRNVLRAVCRRA
                     EEQGLEGFKCLHKGKPKDDNPEALHAYDDNDRVAELLRGGEYSLLGGTSWLWANARFR
                     DSVDVLFIDEAGQMSLADVLAVSAGAKSLVLLGDPQQLEQPQQASHPPGAGASALEHL
                     LGGTKTIAPERGLFLHQTRRLHPEICKFTALAFYENRLTSFAGLERQAVLSPPGSAAA
                     KLGSSGLVYVPVEHDANQSRSLEEVAAIESLVDALTAFGVRYRNAAGEEVPLTRDDIM
                     IVAPYNAQVTALAERLPDVRIGTVDKFQGREAPVVIVSLTTSAPEDAPRGMDFLYSAN
                     RLNVATSRAKALCILVGNPRLFEPDCRTPQQMRLANAFCLYRENARLV"
     gene            5134..>5357
                     /locus_tag="VFL84_11075"
     CDS             5134..>5357
                     /locus_tag="VFL84_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609210.1"
                     /translation="MKPVALPAQVRELLPVSTTRALMAALILYVWTRPTFHLVSYPVR
                     DYGLMGWQLTGIYPLNWLVPLSAAVLLIAA"
BASE COUNT          793 a         1819 c         1859 g          886 t
ORIGIN      
        1 gggattcgtc tggcgatctt ggccgcgctc gagtcggtct acgagtgctt cccgcacatc
       61 acgctgcccc aatacctgat cacgctcgag gtccgcaaag ccgagcgcga cggcaacccg
      121 cacacgctcg cctcgatcgt caagaagctc aagatgccgt tctcaacggc ttcgcgggtc
      181 gtatggtcgc tgaccgagga agggggcgac atcggaatca tcaagtacct gaaccatccg
      241 acggatcgcc ggaaaaagct gctcgtgatc aacgagcgca acgagccgaa cgagatcccg
      301 cgcgcgatca ctcgcgcgat gctcgactac tacggggaag ccgttaagca gctccgtggc
      361 atgtagcaac ggattggcgc cgccagagcg gctgcaggca aagaaaaggg ccgaaccgtt
      421 tccggctcgg ccccgagcca acgctagccg ctagcggggc gcgctgccgg ccgggccgca
      481 acgacgctcg gtgtgcgtcg tcgtgcggtt ctcctggaat tcgccttggg ccttgcggcc
      541 gagcaaggcg ccggcggccg ctccggccgc agtcgcgagg tcgctgtctc cgtctccgag
      601 ctccttgccg atcgcgccgc ccacgacggc gccgatcgcc gtgccggcga cgcggtgctc
      661 gtctttcgga gcgggcgtgt ccgcgacttc ctcgtcccag cattcctggg cgggggccgc
      721 ggccgtgcgc gttgccggcg ccgctcgcgc cggggccgcg gcttgcggct ccgcggcggc
      781 ggtgtcggcg ttgcgttcgc ccaagtactg atagccgagc acgccgccgg ctgtcgccac
      841 gccgattccg gccacgagac ccatcaacat cgacttgttc atagcgatcc tccctagtac
      901 ggcagccact gtgcgcctgg caaaagacct cgatccatga cggaggtcac ggccgccttg
      961 cagcgaattc tagggtgcgg aaggggcttt gcgactgtcc ccaggcagcg acagcggcgg
     1021 caagccatgc gcggcgcggg cccggtcgca acgctcgtta tgtaaaccgt cgacccagga
     1081 cgcctgaaac tcgcggcacg gcgacggccg cagcgcatgg atcgcgcagt ggacgttctt
     1141 gccgatcgtg ccgcgcagcg cgacgcaccg cggcggcgtc ttctcggtgc cgcgcatgac
     1201 ggcgcggtgc ggcgtcagct tcgcggtgag acccgccggc gtgagaccgc ggccggcggg
     1261 atcggcctcg ctccaatgga acgacgcccg gaaaaaggcg cagcaggcgc cgcacgcgag
     1321 gcacggattc cacacggccg cgcacattac accagtcggg cattctcgcg gtacaagcag
     1381 aacgcgttcg cgagccgcat ttgctgcggc gtgcggcagt ccggctcgaa cagccgcggg
     1441 ttgccgacga ggatgcagag cgccttggcg cgcgacgtcg cgacgttcaa gcggtttgcg
     1501 ctgtagagaa agtccatgcc gcgcggcgcg tcctcgggcg ccgacgtcgt cagcgacacg
     1561 atgacgaccg gcgcctcgcg cccttggaac ttgtcgacgg tgccgatgcg cacgtctggc
     1621 aggcgttcgg cgagcgccgt gacctgcgcg ttgtacggcg cgacgatcat gatgtcgtcg
     1681 cgcgtgagcg gcacttcttc gccggcggcg ttgcggtagc gaacgccaaa cgccgtcaat
     1741 gcgtcgacga gcgattcgat ggccgcgact tcctcgagcg agcgggactg attcgcgtcg
     1801 tgctcgacgg gcacgtagac gagccccgag ctgccgagct tggcggccgc cgagccgggc
     1861 ggcgagagca cggcctggcg ctcgagcccc gcgaaggagg tcaggcgatt ctcgtagaac
     1921 gccaaggccg tgaacttgca gatctcggga tgaaggcgcc gcgtctgatg caggaacaaa
     1981 ccgcgctcgg gcgcgatcgt cttagtgccg ccgagcagat gctcgagcgc cgaagcgccc
     2041 gcgccgggcg gatggctcgc ttgctgcggt tgctcgagct gctgaggatc gccgagcagc
     2101 acgaggctct tcgcgccggc cgatacggcg agcacgtcgg ccagcgacat ctgccccgcc
     2161 tcgtcgatga acaacacatc cacgctgtcg cgaaagcgcg cgttcgccca cagccacgat
     2221 gtgccgccga gcagcgagta ctcaccgccg cgcagcagct cggcgacgcg gtcgttgtcg
     2281 tcgtacgcgt gcagcgcctc ggggttgtcg tccttcggct tgcctttgtg caagcacttg
     2341 aagccctcga gcccctgttc ctcggcgcgc cggcatacgg cgcgcaacac gttgcggatc
     2401 gtctcgtggc cgaccgccgt gacgccgact tttttcttgg cctttatcag ctcgaggatc
     2461 atgtgcgagc ccgtgaacgt cttgccggtg ccgggcggtc cctggatcgg cagcacgccg
     2521 tggtcgagct cgaacccgag gcggatcgcc tccttgactt cggcgtccac ctcgatcgct
     2581 cgcggcgtcg ccgccgcggc gcgctgccgc tcgacggcgc tgcgtgccgc ggcgaacgcg
     2641 atcacgcggc ccggcgcggt cgaggcctgg cgttcggccg gcagcaagcc gcgcgagccg
     2701 gatacgagcc gcggcggccg gcgcagtagc agatcgcgcg cggcgcgatg cgggccgtcc
     2761 gcgtcggcgc cgcttgcggc gatgaagcgc ccgatctcga gcagcacggc gggcttcggc
     2821 ttcggcgaga ccatgcggcg cacgaacacg cgctcgggcc gcgtgtcggc gtggcggcta
     2881 ggctgctcga tgtcgacggt gtggcccgca acgtcgagcg cgtcgatctt gccgatcggt
     2941 tgattgatct cgcgcacgca gacgtcgtcg ccgcggcgca cgtcgtgctc ctgcggcggg
     3001 aacgcgtagc gatgaatcgg cttgcgcggc gagccgccga cggtctcgac gaacgtgagc
     3061 cccgcgagcg ccgagcgctc gtcctcgtag tcctcgagcg gcagctcgag caggcgaaag
     3121 tactcccacc acgcggcttt ctcctcgcgg cggtgccatt cgagcagctg cgccatgagc
     3181 cagcgggcct gctcgccttc gctacgctcc gcggcgtcga cgggcacgcc ttcgagcagg
     3241 gcggccatga ctttcagcgt ttcctcggct gtttcggcga cgctgtcgct cgcttcgccc
     3301 gagcgcagct cggggcgctc gagctcgccg ccccactcgc gctcggccgc gacgcgcagc
     3361 tcttcgagcc agtcgcgcag ccgcgcggcg gagacgcagt cgtcgcggtt gtagcgctcg
     3421 acgatcgcga tgtgctccgc gaatttgttc tcgctcggct ccgcgtcgag gccgagctcg
     3481 agctgcggct tggcggccgc gcgcggcgcg gcatcgagcc cgaggtcggc gcgcatctcg
     3541 atggcccatt cgatcgcgcg gcggctcgcc gtcgcgacgc gcaggtcctg cgtgcgcgcg
     3601 aggccgtaga actgctcgag gtccttcaac gagtacgtct cgacgctcgc ccgcagcgag
     3661 tgcttgacga tcgtgtgcag gtcgacgaag agctcggcgc gcaagatcac gtcgagctcg
     3721 ttctcgcggg tcgcatagcg ccccgacaag cgcttgaacg ccgtcggctc gtaggcgccg
     3781 aagtgataga cgtgcatcgc gcggtcgtct tcgaatgccg cgaggatgcg gtcgacgacg
     3841 cgctcgaagg cgcgcttctc ctcggcgggg ttcgccgccc acaagggcgt atacgagccg
     3901 cgcgcgtcgc tgatgccgaa caagtactcg cgcccgccgt cgagcgcgag ccgatcgccc
     3961 tcgagatcga gaaagagatc gccgcgcgac ggcgcaggca ggcgaaaaaa tccgtgctcg
     4021 cggccgagag gcagcacctc gtgctggcgc gtttgcaagc ggcgcgcctt gagctggatc
     4081 gccgcttgat cgcgggcgcg ctcgagcgcg tcgcgcgagc cgcgcgtcgg ctttggcacg
     4141 accttgagct cgccgagccg ctcgagcgtc tcgacgtcga gccgtgtgcg cagctccttg
     4201 atttgcagcc ggctgattcc cgcgacgaaa cagagatgat cgtcggcgcg ccggcgcgcg
     4261 ttgcattcct tccaccagtt gcagacgtcg cagtgctgcg cgggctcggg gtatgtgatg
     4321 gcgtcgcgcg cttcgagggc cgtttcgagc cggtgcttca cgagccggta ataagcggag
     4381 tagtcgtcga gccggtagct ctcgggaacg aatttgtgat gcggcgcgac gacgtgcgct
     4441 tgttgcggcg cgcggccctg cagccgctcg agcagcgtcg agtagacgca gagctgcagg
     4501 atcgtgccgg cgcgcgtctc ggtcgcgagc ttcgcgtccg tgacctcgta cgaccaagcg
     4561 ccgagctcgc tctcgcccgc cactttgcgc aggaagtccg cgcggccgta ccagcggtcg
     4621 tcgccgagcg gcgcctgata gatgacgtcg gcgccgtcgc gcatcgcggc cagcgtggcc
     4681 tcgaagttgt cggcgccggg cgcggtgcgg atttcgacga cgccgagctt ctgcgcgcgc
     4741 aggtgcttca agtagccggc ctcgtgctcg cggccgcgtt ccatgagcaa gtccacgatc
     4801 gggtcgttgc gatgcggcct gcgcgcgcga ccttcggcca ccgcgagctc gagccggctc
     4861 agatggccgc agcccaagtg gttcgcgaga tcggtggctg agagccgcaa cagttcggcg
     4921 acgagacgca tggcgcgatg ataatggcgg gccaccttag ccggtacccg ccgcgaaccg
     4981 atcgcgagct gccgtgcggc ggcgtcaccc tgcgccacag tttgcaggaa ttcgagcatt
     5041 tcgttgccac atttcctttc tagaatcact cgccacgaac ggcgctggtt cttaggagag
     5101 gaaatgtttg cggaacattt ttgctcccgc ctcgtgaaac ccgtcgcgtt gcccgcgcag
     5161 gtgagagagc ttctgccggt atcgacgacg cgcgcgttga tggcggcgct gatcctctac
     5221 gtgtggacga ggccaacgtt tcacctcgtg agctatccgg tccgcgatta cgggttgatg
     5281 ggctggcaac tgaccggcat ctatcctctc aactggctgg tgcccctctc ggccgcggtt
     5341 ttgctgatcg ccgcgtg
//
LOCUS       DASSNN010000599         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_232223, whole genome shotgun sequence.
ACCESSION   DASSNN010000599 DASSNN010000000
VERSION     DASSNN010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232223"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..2151
                     /locus_tag="VFL84_11080"
     CDS             148..2151
                     /locus_tag="VFL84_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HET7609211.1"
                     /translation="MSAAVETGWPVHGGTTTEQRFSPLAQINKDTIAELKPAWFVELD
                     TNRGQEATPIVVDGVLYTSTAWSKVYAVNAKTGERVWYYDPEVPGIAGYKACCDVNSR
                     GLAVAGGKVFLATLDGRLIALDQKTGTPLWSTVTVDQTQSYTITGAPRVIKNKVLIGN
                     SGAELGVRGYVSAYDMETGELAWRFYSVPGDPAKGPDGAASDEAIEKLARPTWFGDRY
                     YQYGGGGTPWDAIVYDEELDQVYVGTGNGSPWSRRQRSEGKGDNLFLSTVLALNPDTG
                     AYIWHYQESPGESWDYTSVQPMMLVDLTIAGTPRKALLHAPKNGFFYVLDRHTGKVIS
                     ANNFVPVNWATSVDVATGRPAITANAFYDDGAFFATPTASGGHNWSPWSYSPRTGLVY
                     FQAQEAYLRYDDLPDWKYVPGGYNLGQDRRPEVVRDDAKNLPAAKFYVLAWDPVENKE
                     RFRVDARGGGVLATAGDLVFQGRGIIDGELVAIDAATGQQVWSYPMPNAAAAAPISYS
                     VDGEQYIAINTGARATGNLQGIVDAQARARQPGRLVAFKLGGAATLPPPPGPAPPPNP
                     PDEVFADDAVRAGELLYADYCARCHGNATRSINVIPDLRRSPALTNKELWRSIVMDGT
                     LTDLGMIGWSHLIEPTQAESIRAYVGRQAVVLRDELAAEPAAP"
     gene            2208..>2544
                     /locus_tag="VFL84_11085"
     CDS             2208..>2544
                     /locus_tag="VFL84_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HET7609212.1"
                     /translation="MTAELNYVVFAGTRRVAAGQLRDVLPVLKRRFDRNRSDVGLVFE
                     VETGRQVEFDLRGTPTEILDRALPAPRRGRGRPKLGVVSREVSLLPRHWAWLEEQSAG
                     ISGALRRLVE"
BASE COUNT          447 a          856 c          862 g          379 t
ORIGIN      
        1 atctcgcaac gccgtagttc cggccgccta gaaaaggcga aggggcccct tatgaaccga
       61 gcgctggcct gcgccgcggc ggcggtgata tgtacgtcct gcgagagccg ctctcccgag
      121 ccggtggact cgggcaacga gggttccgtg tccgccgccg tcgaaacagg atggccggtc
      181 cacggcggca ccacgaccga gcagcgcttc tcgccgctcg cgcaaatcaa caaggacacg
      241 atcgcggagt tgaagcccgc gtggttcgtc gaactcgaca cgaaccgcgg ccaagaggcg
      301 acgccgatcg tcgtcgacgg cgtgctctat acgtccaccg cttggagcaa ggtctacgcc
      361 gtgaacgcga agaccggcga gcgcgtttgg tactacgacc cggaagtgcc gggaatcgcg
      421 ggctacaagg cgtgctgcga cgtgaacagc cgcgggctcg cggtcgcggg cggcaaagtg
      481 ttcctcgcga cgctcgacgg ccgcctgatc gcgctcgatc agaagaccgg cacgccgctg
      541 tggagcaccg tcacggtcga tcaaacgcag tcctacacga tcacgggcgc gccgcgcgtg
      601 atcaagaaca aggtgctgat cggcaactcg ggcgccgagc tcggcgtgcg cggctacgtg
      661 agcgcctacg acatggagac cggtgagctc gcgtggcgct tctattcggt gcccggcgat
      721 cccgcgaagg gccccgatgg cgccgcgtcc gacgaggcga tcgagaagct cgctcgcccc
      781 acttggttcg gcgacaggta ctaccagtat ggcggcggcg gcacgccgtg ggacgcgatc
      841 gtctacgacg aggagctcga tcaggtctac gtcggcacgg gcaacggtag cccgtggagc
      901 aggcggcagc gctcggaagg caagggcgac aacctgttcc tctcgaccgt gctcgcgctg
      961 aatcccgata ccggcgccta catctggcac taccaggaat cgcccggcga gtcgtgggac
     1021 tacacgtcgg tgcagccgat gatgctcgtc gatctcacga tcgcgggcac accgcgcaag
     1081 gcgctgctgc acgcgccgaa gaacggcttc ttctacgtgc tcgaccgcca tacgggcaag
     1141 gtgatctcgg cgaacaattt cgtgcccgtg aactgggcga cgtccgtcga cgtcgcgacg
     1201 gggcgaccgg cgatcacggc gaacgcgttc tacgacgatg gcgcgttctt cgcgacgccg
     1261 accgccagcg gcggccacaa ctggtcgccg tggtcgtaca gcccgcgcac ggggctcgtc
     1321 tatttccagg cgcaagaggc gtacttgcgc tacgacgacc tgccggattg gaagtacgtg
     1381 ccgggcggct acaacttagg ccaagaccgg cggccggaag tcgtgcgcga cgacgcgaag
     1441 aacttgccgg ccgcgaagtt ctacgtgctc gcctgggatc ccgtcgagaa caaggaacgc
     1501 ttccgcgtcg atgcgcgcgg cggcggcgtg cttgcgaccg cgggcgatct cgtcttccaa
     1561 gggcgcggca tcatcgacgg cgagctcgtt gcgatcgatg cggcgacggg ccaacaggtt
     1621 tggagctatc cgatgccgaa cgccgcggcc gcggcgccga tctcgtatag cgtcgacggc
     1681 gagcaataca tcgcgatcaa caccggcgcg cgcgcgacgg gcaacctgca gggcatcgtc
     1741 gatgcgcagg cgcgcgcgcg gcaaccggga cggctcgtcg cgttcaagct cggcggcgcg
     1801 gccacgctgc cgccgcctcc gggccctgcg ccgccgccga acccgccgga cgaagtgttc
     1861 gcggacgacg cagtgcgcgc gggcgagctg ttgtacgccg attactgcgc ccgttgccac
     1921 ggcaacgcga cgcgcagcat caacgtcatt cccgacttgc gacgttcgcc ggcgctcacg
     1981 aacaaggagc tatggcgctc gatcgtgatg gacggcacgc tcacggatct cggaatgatc
     2041 ggctggagcc acctcatcga gccgacgcaa gccgagagca tccgcgcata cgtcggccgc
     2101 caagccgtcg tgcttcgcga cgagctcgct gcagagcccg cagcgcccta gctattctat
     2161 tttaacccgg gtaaattacg gccgtaattt gcgcccggga aaaatagatg accgcagagc
     2221 tcaactacgt cgtcttcgcc ggcacacgcc gcgtggccgc agggcagctg cgcgacgtcc
     2281 ttcccgtgct gaagcggcgt ttcgaccgca accgctcgga cgtggggctc gtgttcgaag
     2341 tcgagaccgg ccggcaagtc gaattcgact tgcgcggcac gcccaccgaa atcctcgacc
     2401 gcgcgctgcc ggcgccgcgc cgcggccgcg gacggcccaa gctcggcgtt gtgagccgag
     2461 aagtctcgct gctgccgcgc cactgggcct ggctcgagga gcaaagcgcg ggaatctcag
     2521 gcgcgctgcg ccggctcgtc gagc
//
LOCUS       DASSNN010000600         7143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_479795, whole genome shotgun sequence.
ACCESSION   DASSNN010000600 DASSNN010000000
VERSION     DASSNN010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479795"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="VFL84_11090"
     CDS             <1..1462
                     /locus_tag="VFL84_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7609213.1"
                     /translation="GTGLGLAISRELSKLLGGEIKLVSTPGEGSTFTLYLPLSTPRIS
                     RPTRPSPGEARAPLPPPAPPAPKPPVQTLYAEDDSPPSTLNEAGDDRASIQPGDRTIL
                     IVENDLAFAKVLLEAARRQGFKGLVSATAAAAISLINEYMPTIVSLDIFLPDMPGWRI
                     LDRLKADLSTRHIPICIVSTDDSRDRALSSGAIGFITKPVASSDVIDEAIGQLADYVG
                     RPRKRVCLAMAPGGDLEIAQSRFAEGSVDVIATAEAAEICELLRKDQIDCLVVESGAT
                     DLQPADLIEVVEHRVLSRQLPIVIYGSGGGDALSRWRRRDGMFALREARSLERLVDTV
                     YFSLHRSLQELSDGERQAIEDLHRDSRVLEGRKALVVDDDMRNIFALSTVLHDHGMEI
                     ISANNGREAIRLVQEDPSIDIVLMDIMMPEMDGMTTMREIRKIEQRKDLPIIAVTAKA
                     MKGDREKCIEAGAWDYLSKPVDTIHLLGVLKSWLHR"
     gene            1470..3593
                     /locus_tag="VFL84_11095"
     CDS             1470..3593
                     /locus_tag="VFL84_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7609214.1"
                     /translation="MRPHPLPMPMEEKVNILVVDDLPDKRLAFREVLEDLDENLVLVG
                     SGAEALRELLEREFAVIVLDVNMPDIDGIETAQLIRKHGKTSHTPIIFVTAYADEMQT
                     ERGYELGAVDYILTPVVPAILRSKVRVFVDLYRAQRRAAKVARAEAARAAAESAQRRS
                     DLIALASRELSSSLDLRSSMERLLEVLVPNVGGCAVLSVDTDNGTVGLYRQDREPARL
                     LETLGVLPPAVRRAMAEVQNGGYSLEIRTDAPTSGQWPPWLRVAHVLPLKVGERTVGA
                     LTVGTVDHESAMIDDRGLLEIATRAAIAFDNAQLYSSLGREMARAKSAEEKLQEANRR
                     KDEFLAMLSHELRNPLAPIVTSAQVLDKVAPGDTRIAWVREVIERQVTHLAGLVDDLL
                     DVSRITQGKITLNREAVELGKILEHSLEIVRPLIDSKRHVLSVNVPDTPVWVFGDFSR
                     LSQIFSNVLHNAAKYTSDGGSIELNVIVDRGSVSVRVRDNGIGIEPQFLPHVFEVFTQ
                     GYRGLDRSQGGLGVGLAVVRRLIELHQGEVDIMSDGPGLGTEVSIRLPCISEVVQPTG
                     FPEEPRSARGGKRILIVDDNADAAESIAVLMQIEGHEVKTVTDAMQALACLDAFAPQV
                     AIIDIGLPGMNGYELAAGIRANRSLPKPLLIALTGYGQAEDFDRSRDAGFDHHFVKPA
                     EIQAIQAAIDEAPDDAEVEERRTLV"
     gene            3596..3730
                     /locus_tag="VFL84_11100"
     CDS             3596..3730
                     /locus_tag="VFL84_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609215.1"
                     /translation="MGSIVTPPPEVARKRRGGDADSLEEIVGHVPADLGPAESPRAAG
                     "
     gene            3717..>7143
                     /locus_tag="VFL84_11105"
     CDS             3717..>7143
                     /locus_tag="VFL84_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7609216.1"
                     /translation="MPQGNAAVAQTGADWLQGGGEMGARVRALDWSATALGPIGDWPQ
                     SLRSPLSMMLASKAQIILFWGPDYVVFYNDAYRPVFGAKHPNMLGMPGGVAWSEIWDS
                     GVNLRGLLDGVVRTGEAFSARDMLFVLERYGFDEETYFDVSYDPVRAESGAIAGVFCI
                     VTETTGRVVGERRLALLKDLAAHNSTARTQRDACVLAMETLATKQQDIVFALAYLDGQ
                     LQACTPGAEAALAAADGQQLMDLPFLSGNLVVGLNPRRPFDDLYRSFVTLVADQIAAA
                     VANARAYEDERKRAEALAEIDRAKTAFFSNVSHEFRTPLTLMLGPLEDLLAGAHGPLS
                     GGVEAAASLAHRNSLRLLKLVNSLLDFARIESGRADACYEPTDLAVETAELASVFRSA
                     IERAGLELAVDCQPLGEPVYVDRDMWEKIVLNLLSNALKFTFQGVIHVALRRHGDRAE
                     LRVSDTGVGIAEADLPKLFQRFQRVRNARSRTHEGSGIGLALVRELVKLHGGEIDVES
                     RERAGTAFTVRVPLGKAHLPSERVGAARQLASTRVGAQVFVEEALRSLPGKGEEPDPT
                     VSVQIPKMARKRLLLVDDNVDMREYVRRMLERHYDVVAAGDGHEALAAIRESVPDLVL
                     TDVMMPRLDGFGLLAAIRADSRTQSLPVIMLSARAGEEARLEGLAAGVDDYLIKPFSA
                     RELLARVGSHLALAEQRGRLEQALRYHAEQVETLLECAPLGIYLIGPDFRIRAVNPVA
                     RLAFGDIPGGLEGRDFGEVVNLLREESYADQVVRLFRHTLATGEPYFTREQALTRRES
                     GVQEYYEWRVERITLPDGERGLVCYFRDISAQVAARTAVEESREALKQADRRKDEFLA
                     TLAHELRTPLAPIHNALEILRLSGREGAVARSVYEMMSRQVSHMVRLVDDLMEVSRIT
                     RGKIDLEKQRVQLADVVANAVETTRPLVDSAGHLLTVDLGGASLEIDADPVRLAQVFS
                     NLLNNAAKYTPNGGQITLRAEQNGGYVVVSVRDNGTGIRGDVLAHVFDAFVQGDRSYS
                     RAKGGLGIGLTLARSIVALHGGTIEARSAGLGQGSEFVVRLPLPPPSVEGSAVESSRP
                     QTRIAGQRILVVDDNVDAAESLGALLRCLGAEVVTVHDGPAALEALRTETPAAS"
BASE COUNT         1165 a         2428 c         2438 g         1112 t
ORIGIN      
        1 cggcacgggc ctaggcctgg cgatcagccg cgagttgtcg aagctgctcg gcggcgagat
       61 caagctcgtc agcacgcccg gcgaaggcag cacgttcacg ctgtacctgc ctctgagcac
      121 gccgcggatc agccgcccga cgcggccgag cccgggcgag gcgcgagcgc cgttgccgcc
      181 gccggcgccg ccggcgccga agccgccggt gcagacgctc tatgccgagg acgactcgcc
      241 gccttctacg ttgaacgaag ccggcgacga tcgcgcgagc atccagcccg gcgaccgcac
      301 gattctgatc gtcgagaacg acctcgcatt cgcgaaggtg ctgctcgagg ccgcgcggcg
      361 ccaaggcttc aagggcctcg tcagcgcgac cgccgcggcc gcgatctcgt tgatcaacga
      421 gtacatgccg acgatcgtgt cgctcgacat ctttctgccc gacatgccgg gttggcgcat
      481 cctcgatcgg ctcaaggccg atctgtcgac gcgccacatc ccgatctgca tcgtctcgac
      541 cgacgattcg cgcgaccgcg cgctgtcgtc gggggcgatc ggcttcatca cgaagcccgt
      601 cgcatcgagc gatgtcatcg acgaagcgat cgggcagctc gcggactatg tcgggcggcc
      661 gcgcaagcgc gtctgtctcg caatggcgcc gggcggcgac ctcgagatcg cgcaatcgcg
      721 cttcgccgaa ggctcggtcg acgtcatcgc cacggcggaa gcggccgaga tttgcgagct
      781 cttacgcaag gaccagatcg attgcctcgt cgtcgagtcc ggcgccaccg acttgcagcc
      841 ggccgacctg atcgaggtcg tcgagcaccg cgtgctgtcg cggcagctgc cgatcgtgat
      901 ctacggctcc ggcggcggcg acgcgctatc gcgctggcgc cggcgcgacg gcatgttcgc
      961 gctgcgcgag gcgcgctcgc tcgaacggct cgtggacacg gtgtacttct cgctgcaccg
     1021 ctcgctgcag gagctgtcgg acggcgagcg gcaggcgatc gaggacttgc atcgcgacag
     1081 ccgcgtgctc gagggccgga aggcgctcgt cgtcgacgac gacatgcgca acatcttcgc
     1141 gctctccacc gtgctgcacg accacggcat ggagatcatc tccgctaaca acggccgcga
     1201 ggcgattcgt ctcgtgcagg aggacccgtc gatcgacatc gtgctgatgg acatcatgat
     1261 gccggagatg gacggcatga cgacgatgcg cgagatccgc aagatcgagc agcgcaagga
     1321 tttgccgatc atcgccgtca ccgcgaaggc catgaagggc gatcgcgaga agtgcatcga
     1381 agccggcgcc tgggactatc tctcgaagcc ggtcgacacg atccacttgc tcggcgtgct
     1441 gaagagctgg ctgcaccgct aagggctcca tgagacctca cccgctgccg atgccgatgg
     1501 aagagaaagt caacatactc gtcgtcgacg atttgcccga caagcgcctc gcattccgcg
     1561 aggtgctcga ggacctcgac gagaacctcg tgctcgtcgg ttccggtgcc gaagcgctgc
     1621 gcgagctgct cgagcgcgag ttcgcggtca tcgtcctcga cgtcaacatg cccgacatcg
     1681 acggcatcga gaccgcgcag ctgatccgca agcatggcaa gacgtcgcac acgccgatca
     1741 ttttcgtcac ggcctatgcc gacgagatgc agacggagcg aggctacgag ctcggcgccg
     1801 tcgactacat cctgacgccg gtcgtgcctg cgatcctgcg cagcaaggtg cgcgtgttcg
     1861 tcgatctcta ccgtgcccaa cggcgagcgg ccaaggttgc gcgcgccgag gccgcgcgcg
     1921 cggcggccga gtcggcgcaa cgacgctccg acttgatcgc gctcgcgagc agggagctgt
     1981 cgtcgtcgct cgacctgcgc tcgagcatgg agcggctgct cgaggtgctc gtgccgaacg
     2041 tcggcggctg tgccgtccta tccgtcgaca cggacaacgg aaccgtgggc ttgtacaggc
     2101 aggaccgcga gcccgcgcgc ctgctcgaga cgctcggcgt gctgccgccc gctgtccgcc
     2161 gtgcgatggc cgaggtccag aacggcggct actcgctcga gatccgcacc gacgctccga
     2221 cgtccggcca gtggccgccg tggctgcgcg tcgctcacgt gctgccgctc aaggtcgggg
     2281 aacgcaccgt cggcgccctc acggtcggaa cggtcgatca cgagagcgcc atgatcgacg
     2341 accgcggcct gctcgagatc gcgacacgcg cggcgatcgc gttcgacaac gcgcagctct
     2401 acagcagcct gggccgcgag atggcgcgcg cgaagtccgc cgaggagaag ctgcaggagg
     2461 cgaaccgccg caaggacgag tttctagcga tgctctcgca cgagctgcgc aatccgctcg
     2521 cgccgatcgt gacctccgcc caggtgctcg acaaggtagc gccgggcgac acgcgcatcg
     2581 cctgggtccg cgaggtcatc gagcggcaag tcacgcatct cgcgggtctc gtggacgact
     2641 tgctcgatgt ctcgcgcatc acccaaggca agatcacgct gaatcgcgag gccgtcgagc
     2701 tgggcaagat cctcgagcac agcctcgaga tcgtgcggcc gctcatcgat tcgaagcgcc
     2761 atgtgctgtc ggtcaacgtg ccggacacgc cggtctgggt attcggcgac ttctcccgtt
     2821 tgagccagat cttcagcaac gtgctgcaca acgccgccaa gtacacgagc gacggcgggt
     2881 ccatcgagct caacgtcatc gtcgatcgcg gcagcgtcag cgttcgcgtg cgcgacaacg
     2941 gaatcggtat cgagccccaa ttcctgccgc acgtgttcga agtgttcacg caaggttacc
     3001 gtgggttgga tcgcagccag ggtgggctcg gcgtcggcct cgccgtcgtc cgccgcctga
     3061 tcgagctgca tcagggagag gtcgacatca tgagcgacgg tccgggactc ggcaccgagg
     3121 tcagcatccg tctgccgtgc atcagcgagg tcgtgcagcc gaccggcttc ccggaggagc
     3181 cgcgctcggc gcgcggcggc aagcgcatcc tcatcgtcga cgacaacgcc gacgcggcgg
     3241 agtcgatcgc ggtgctgatg caaatcgaag ggcacgaggt caagacggtc acggacgcca
     3301 tgcaggcgct cgcctgcctc gacgcgttcg cgccgcaagt cgccatcatc gacatcggct
     3361 tgccgggcat gaacggttac gagctcgcgg ccggcattcg ggccaaccgc tcgctgccga
     3421 agccgttgct gatcgcgctg acgggatacg ggcaggccga ggacttcgac cgctcgcgtg
     3481 acgcaggctt cgatcatcac ttcgtgaagc ccgcggagat tcaggccatt caagccgcaa
     3541 tcgacgaagc tcccgacgac gccgaggtcg aagagcgacg aacgctggtt tgagcatggg
     3601 tagcatcgtg acgccgccgc ccgaagttgc gcgaaagcgg cgaggcggcg acgccgactc
     3661 gctcgaggaa atcgtcgggc acgtgccggc cgatctcggg cccgcggaga gcccgcgtgc
     3721 cgcagggtaa cgccgcggtt gcccagacgg gcgccgattg gttgcaaggc ggcggcgaga
     3781 tgggcgcgcg cgtccgcgcc ctcgactggt cggcgaccgc gctcggcccc atcggcgatt
     3841 ggccgcagag cttgcgcagt ccgttgagca tgatgctggc ctcgaaggcg cagatcattc
     3901 tgttctgggg ccccgactac gtcgtcttct acaacgatgc gtatcggccc gtcttcggcg
     3961 caaaacatcc caacatgctc ggcatgccgg gcggtgtcgc gtggagcgag atctgggaca
     4021 gcggcgtcaa tctgcgcggg ctgctcgacg gagtcgtgcg cacgggcgag gcattcagcg
     4081 cgagagatat gctgttcgtt ctcgagcgct acggcttcga cgaagaaacc tacttcgacg
     4141 tctcgtacga cccggtgcgc gcagagtccg gcgccatcgc cggtgtcttc tgcatcgtca
     4201 ccgagacgac gggacgcgtc gtcggcgagc ggcggctcgc actgctcaag gatctcgctg
     4261 cgcacaattc gacggctagg acgcagcgcg acgcctgtgt gctcgcgatg gaaacgctcg
     4321 cgacgaagca acaggacatc gtgttcgcgc tcgcgtatct cgacggccag ctgcaggcat
     4381 gcacgcccgg agccgaggcc gcgcttgcgg ccgcggacgg gcagcagctc atggacctgc
     4441 cttttctgtc cggcaacctc gtcgtcggcc tgaatccgcg gcggccgttc gacgacctat
     4501 accgttcgtt cgtgaccttg gtcgcggacc agatcgccgc cgcggtcgcg aatgcgcggg
     4561 cttacgagga cgagcgcaag cgcgccgagg ctctcgcgga gatcgaccgc gccaagacag
     4621 ccttcttctc gaacgtcagc cacgagttcc gcacgccgct cacgttgatg ctcggtccgc
     4681 tcgaggacct gctcgccggc gcgcacggcc cgctgtcggg cggcgtcgag gccgcagcgt
     4741 cgctggctca ccgcaacagc ctgcggctgc tgaagctcgt caactcgctg ctcgactttg
     4801 cgcgcatcga gtccggccgc gccgacgctt gctacgagcc cacggatctc gccgtcgaga
     4861 cggccgagct cgcgagcgtg ttccgctcgg ccatcgagcg tgccggcctc gagctcgccg
     4921 tcgactgcca gccgctcggc gagcccgtct acgtggaccg cgacatgtgg gagaagatcg
     4981 tgctgaatct tctctcgaac gcgctcaagt tcacgttcca gggcgtgatc cacgtcgcgc
     5041 tgcgcaggca cggcgaccgc gcggagctgc gcgtcagcga caccggcgtc ggtatcgccg
     5101 aggccgatct gccgaagttg tttcaacgct ttcagcgcgt gcgcaacgcg cgctcgcgca
     5161 cgcacgaggg ctccggaatc ggcctcgcgc tcgtgcgcga gctggtcaag ctccacggcg
     5221 gcgagatcga cgtcgagagc cgcgagcgag ccggcacggc gttcacggtg cgcgtgccgc
     5281 tcggcaaggc gcatttgccg tccgagcgcg tgggcgccgc gcgtcagctc gcgtcgacgc
     5341 gcgtcggcgc tcaggtcttc gtcgaagaag cgctgcgctc gctgcccggg aagggcgagg
     5401 agccggatcc gacggtcagc gtccagatac cgaagatggc gaggaagcgc ctgctgctcg
     5461 tcgacgacaa cgtggacatg cgcgagtacg ttcgccgcat gctcgagcgg cactacgacg
     5521 tcgttgcggc cggcgacggc cacgaagcgc tcgccgcgat ccgcgagagc gtgccggatc
     5581 tcgtcctcac cgacgtcatg atgccgcggc tcgacgggtt cggcttgctg gccgcgattc
     5641 gggccgactc gcgcacgcag tcgctgcctg tcatcatgct gtcggcgcgc gcaggcgaag
     5701 aagcgcggct ggaaggcctc gccgccggcg tcgacgacta tctgatcaag ccgttcagcg
     5761 cgcgtgagct gctcgcgcgc gtcggctcgc atctcgctct cgcagagcag cgcggccggc
     5821 tcgagcaagc gctgcgctac cacgccgagc aggtcgagac gctgctcgag tgcgcgccgc
     5881 tcggcatcta tctgatcggt cccgactttc ggatccgcgc ggtcaatccg gttgcgcggc
     5941 tcgcgttcgg cgacatcccg ggcggtctcg aaggccgcga ctttggcgag gtcgtgaacc
     6001 tcctacgcga ggagtcgtac gccgaccagg tcgtgcggct gttccgccac acgttggcga
     6061 ccggcgagcc gtacttcacg cgcgagcaag ccctgacgcg tcgcgagagc ggcgtgcagg
     6121 agtactacga gtggcgcgtc gagcgcatca cgctgcccga cggcgagcgt gggctcgttt
     6181 gctacttccg cgacatctcc gcgcaggtcg cggcgcgcac ggccgtcgag gaaagccgag
     6241 aggcgttgaa gcaagccgat cggcgcaagg acgagttcct cgcgacgctc gcgcacgagc
     6301 tgcgcacgcc gctagcgccg attcacaacg cgctcgagat cttgcggctc tcgggccgcg
     6361 agggcgccgt cgcgcgcagc gtgtacgaga tgatgagtcg acaagtcagc cacatggtca
     6421 ggctcgtcga cgatctcatg gaagtgtcgc ggatcacgcg cggcaagatc gacctcgaga
     6481 aacagcgtgt ccagctggcg gacgtcgtcg cgaatgcggt cgagacgacg cgcccgctcg
     6541 tcgactcggc gggacacctg ttgacggtcg acctcggcgg cgcgtcgctc gagatcgatg
     6601 cggaccccgt gcgcctcgcg caagtcttca gcaacctgct caacaacgcg gcgaagtaca
     6661 cgccgaacgg tggccagatc acgctgcgcg ccgagcaaaa cggcggctac gtagtcgtgt
     6721 cggtgcgcga caacggcacc ggcattcgag gggacgtgct cgcgcacgtc ttcgacgctt
     6781 tcgtccaggg cgaccgcagc tacagccgcg cgaagggcgg tctcggcatc ggcctcacgc
     6841 tcgcgcgcag catcgtcgcg ctgcacggcg gcacgatcga ggcgcgcagc gccggcttgg
     6901 ggcagggcag cgagttcgtc gtgcgcctgc cgctgccgcc cccgagcgtc gagggctccg
     6961 ccgtcgagtc gagccgcccg cagacgcgca tcgcgggcca gcgcatcctc gtcgtcgacg
     7021 acaacgtcga cgccgcggag agcttgggcg cgttgctacg ctgcttggga gcggaggtcg
     7081 tgaccgtgca cgacggcccg gccgcgctcg aggcgctgcg gacggagacg ccggccgcgt
     7141 cgg
//
LOCUS       DASSNN010000601         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_606772, whole genome shotgun sequence.
ACCESSION   DASSNN010000601 DASSNN010000000
VERSION     DASSNN010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606772"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..1183)
                     /locus_tag="VFL84_11110"
     CDS             complement(227..1183)
                     /locus_tag="VFL84_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7609217.1"
                     /translation="MNDSIDRRALLRNASLLVTGAALAKAVAAQEAPPAPQPPFPGFT
                     QHDIPTSGGVTIRAVKGGNGPPLLLLHGAPQTHYTWRDVAPRLAEEYTVVAADLRGYG
                     DSSQPQGLPDHSNYSKRAMAQDQVDVMKHFGFERFALVGQDRGGRVAHRLTLDHPDAV
                     TRAVFIDIVPTYYLYTHVTLAFVQSYPHWFSLLAPSPAGENAAVALQGGPANAAASPA
                     QLEYRRRNATPEGRHAMCEDYRAAASIDLEHDAADLDHRIRCPLHVLWAQDGAMDRLY
                     DVLAIWRERARNVTGKGMPGGHNMQEGAPNEVLAELRTFLRA"
     gene            complement(1183..>2191)
                     /locus_tag="VFL84_11115"
     CDS             complement(1183..>2191)
                     /locus_tag="VFL84_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7609218.1"
                     /translation="PPRFIPPSESPAGLSRGIGIIRAGWPAGRGKRFPADGSKTALLY
                     AAREGHLDIARLLVDHGASLDLADGNGVTPLISAIINASIVRVNRTGTSDHLKIAQLL
                     LDAGADVNAADWYGETPLWAAVDVRNLELGRDGNDRNVRDEALALVQRLLEAGANPNA
                     RTREFPHDRRFLVTIGSVAWVDLTGQTPFLRAAAAGDLEVMRLLLAHGADPNIATDAG
                     TTPLMVAAGVNWSVAETSDFGNEALLEAVKLAHSLGNDVNAVNSMGIAAVHGAANRGA
                     NDVIEYLAANGARLDAADAQGRTPLDWAAGVFLATHPPVRKPETIALLERLLKEQRGS
                     K"
BASE COUNT          321 a          741 c          762 g          367 t
ORIGIN      
        1 gctcaagtcg cgccgatcgc agccactgcc cgtataccgc ggcaagagtg gtctcgcgaa
       61 aggcatcgat cccacctcca atcgctcgat gcttgacgct gcgagcgatg gctcctgaca
      121 tcaacgttgg taaccctcga ccgcgggttc agccgcctcc tcggcaaggg cgagctgtta
      181 ttgcttcggc cttagccggt cccaccagct ctacagttcg agcgcctcac gcgcgcagga
      241 acgtacgcaa ctcggcgagt acctcgttgg gcgcgccttc ctgcatgttg tggccccccg
      301 gcatgccctt gcccgtgacg ttgcgcgcac gctcgcgcca gatcgcgagc acgtcgtaga
      361 gccgatccat ggcgccgtcc tgcgcccaga gaacgtgcag cggacagcgg atgcggtggt
      421 cgagatcggc cgcgtcgtgc tcgaggtcaa tcgacgcggc agcgcggtag tcctcgcaca
      481 tggcatggcg gccttcgggc gtcgcgttcc tgcgccggta ctcgagctgc gccggcgacg
      541 ccgcggcatt cgccggaccc ccttgcagcg cgaccgccgc gttctccccc gccggcgacg
      601 gcgccagcag gctgaaccag tgcggatacg actggacgaa cgcgagcgtc acgtgcgtgt
      661 agaggtagta cgtcggcacg atgtcgatga aaacggcgcg cgtgacggcg tcgggatgat
      721 cgagcgtcaa gcggtgcgcg acgcggccgc cgcggtcctg tccgactaga gcgaagcgct
      781 cgaagccgaa gtgcttcatc acgtcgactt ggtcttgcgc catcgcgcgc ttcgaataat
      841 tcgagtgatc cggcaagcct tgcggctggc tgctgtcgcc gtagccgcgc aagtcggccg
      901 cgacgaccgt gtactcttcg gcgagccgcg gcgcgacgtc gcgccacgtg taatgcgtct
      961 gcggcgcacc gtgcagcagc agcagcggcg gcccgttgcc gcctttgacg gctcggatcg
     1021 tgacgccgcc gctcgtcgga atgtcgtgct gcgtgaaccc cggaaacggc ggctgtggcg
     1081 caggcggagc ctcttgggcc gcgactgctt ttgcgagtgc ggctcccgtc acgagcagcg
     1141 aagcgttgcg cagcaaggcg cggcggtcga tcgagtcgtt cattacttgg atcctcgttg
     1201 ttctttgagt aagcgttcga gcaaagcgat cgtctcgggc ttgcggaccg gcgggtgcgt
     1261 cgcgaggaac acacccgcgg cccaatcgag cggcgtgcgg ccttgcgcgt cggccgcgtc
     1321 gagccgcgcg ccgttcgccg cgagatattc gatgacgtcg ttcgcgccgc gattcgcggc
     1381 gccgtgaacc gccgcgatgc ccatcgagtt cacggcgttg acgtcgttgc cgagcgagtg
     1441 cgcgagcttg acggcttcga gcagcgcttc gttgccgaag tcggacgtct ccgcgacgga
     1501 ccagttcacg ccggccgcga ccatcagcgg cgtcgtgccg gcgtcggtcg cgatgttcgg
     1561 gtcggcgccg tgcgcgagca gcaagcgcat gacctcgagg tcgccggccg cggccgcgcg
     1621 caagaacggc gtctggccgg tgaggtcgac ccaggctacc gagccgatcg tgacgagaaa
     1681 gcgccggtcg tgcggaaatt cacgcgtccg cgcgtttggg tttgcgcccg cttcgagcaa
     1741 gcgctgcacg agcgcgagcg cctcgtcgcg cacgttacgg tcattgccgt cgcggccgag
     1801 ctcgaggttg cgcacgtcga ccgcggccca gagcggcgtc tcgccgtacc agtccgccgc
     1861 attcacgtcg gcgccggcat cgagcaagag ctgcgcgatc ttcaaatgat cgctcgtgcc
     1921 ggtgcggttc acgcggacga tgctcgcgtt gatgatcgcg ctgatcagcg gcgtgactcc
     1981 gttgccgtcc gcgaggtcca agcttgcgcc gtggtcgacg agcagccgcg cgatgtcgag
     2041 atggccctcg cgcgccgcgt acagcagcgc cgttttcgag ccgtcggcgg gaaagcgctt
     2101 cccgcggccc gcgggccagc ccgcccggat gatgccgatg cctcgcgaca gaccggcggg
     2161 cgactcgctc ggcgggatga agcgcggcgg c
//
LOCUS       DASSNN010000602         5017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_606868, whole genome shotgun sequence.
ACCESSION   DASSNN010000602 DASSNN010000000
VERSION     DASSNN010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606868"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFL84_11120"
     CDS             <1..211
                     /locus_tag="VFL84_11120"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7609219.1"
                     /translation="IGVEQTVEAPDAVIVEGRYTGVYKPTGRRMDVQVCHVWRFRGER
                     IASFHQYVDTAALRRVMARDDAAAA"
     gene            complement(224..571)
                     /locus_tag="VFL84_11125"
     CDS             complement(224..571)
                     /locus_tag="VFL84_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7609220.1"
                     /translation="MKYKGSCHCGRVAFEVEGAIESAMSCNCSMCERKGSLLWFVPRA
                     NLRLTTPESSLSTYTFNKHVIKHRFCSNCGIHPFGEGTDQKGNARAAINIRCLEGIEL
                     DKIPVKHFDGRSH"
     gene            complement(555..1043)
                     /gene="msrB"
                     /locus_tag="VFL84_11130"
     CDS             complement(555..1043)
                     /gene="msrB"
                     /locus_tag="VFL84_11130"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET7609221.1"
                     /translation="MSAVREPSPAELRWKHVVELARSGNEPPPRRVEKSDAEWRAQLS
                     DEQYQVARKKGTERPFSSEMCALFTPGRYHCVCCDTPLFDSTSKFDSRTGWPSFTQPL
                     NVGVVAYHLDTSYGMQRVEALCNVCDAHLGHVFPDGPPPSGLRFCINAVSLKKASDEV
                     QR"
     gene            complement(1047..1565)
                     /gene="msrA"
                     /locus_tag="VFL84_11135"
     CDS             complement(1047..1565)
                     /gene="msrA"
                     /locus_tag="VFL84_11135"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET7609222.1"
                     /translation="MSQTETAILAGGCFWGMQDLIRKLPGVVSTRVGYSGGDVPHATY
                     RNHGTHAEAIEVVFDPKRLSYRELLEFFFQIHDPTTKNRQGNDVGMSYRSGIYYLSDE
                     QREIAEDTIADVDASKLWPGRVVTEVKSAGPFWDAEPEHQDYLLRYPGGYTCHFPRPN
                     WKLSRRAAARTG"
     gene            complement(1630..2832)
                     /gene="rocD"
                     /locus_tag="VFL84_11140"
     CDS             complement(1630..2832)
                     /gene="rocD"
                     /locus_tag="VFL84_11140"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HET7609223.1"
                     /translation="MSDSSALIAEAERVSAPNYSPLPVVLARGEGVWVWDVEGRRYLD
                     CISAYSSLNQGHRHPRIVAALVEQAGQLTLTSRAVHNDRMGPFLNELCALAGFEMALP
                     MNTGAEAVETAIKLARKWGYRVKGVAHDRAEIVVCRNNFHGRTTTIVGFSSEAQYRED
                     FGPFTPGFTLVDFGDLAALRAAITSNTVAFLVEPIQAEAGIIMPPEGYLAAARALCRE
                     RNVLFVLDEIQTGLARTGKMFAHDHEPGAEPDMLVLGKALGGGVYPVSAVLASRAIMT
                     LLKPGDHGSTFGGNPLAAAVGHASLRVLIRERLAERAAEQGEYLFGKLRALGAPCIAE
                     IRGRGLLVGIEIKSASGSARAYCERLLERGVLAKDTHEQVVRLAPPLIVERAELDWLV
                     EQLRAVLR"
     gene            complement(2829..3782)
                     /gene="rocF"
                     /locus_tag="VFL84_11145"
     CDS             complement(2829..3782)
                     /gene="rocF"
                     /locus_tag="VFL84_11145"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HET7609224.1"
                     /translation="MTTADRSSASVALIGVPTDVGAGKRGASMGPEALRVAGIDKALS
                     RLGRGVIDRGNVTGPRNPENPRCGDYRHLPEVAAWCRGVHDAVYESLTAGEIPLTMGG
                     DHSAAIGSVAAVARYCAERERPLAMLWLDAHADFNVASSSPSGNIHGMPVGIIAGHGP
                     PELVGLAGFHPMLAADRIVQIGIRSVDASEKELVVDSGMVVYDMRRIDELRMRTVMEQ
                     ALAHLAERDVHLHVSFDVDFLDPSIAPGVPSTVPGGPTYREAQLCMEMIYDSGLMRSL
                     DVMELNPAYDEGNRTAELAVELVQSLFGLQILARAADKYTL"
     gene            complement(3848..4609)
                     /locus_tag="VFL84_11150"
     CDS             complement(3848..4609)
                     /locus_tag="VFL84_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609225.1"
                     /translation="MAEHGSSSSDDEALSALIDGALPSDEAAALRARMLREPDLAARF
                     AAMERANRAVGDAYRDVVDEPLPERVLELLRAPQAREGKVVDLAARRQRRVAPAWLPH
                     AAAAGVALAIGLGLGLTLSQRSDGPSGLLAATGPVEQGSPLHELLESSPSGEPRDVGG
                     ATAEARYTFRAQGGEWCRELAVSSGASRNAAVACRRGGVWRIDLVGVEAADGEVYRPA
                     GGDSPVAGAVDALIDGEPLEPEAERALVGRGWAGD"
     gene            complement(4602..>5017)
                     /locus_tag="VFL84_11155"
     CDS             complement(4602..>5017)
                     /locus_tag="VFL84_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7609226.1"
                     /translation="DLMQNTVERALQRGWPSGVEPLAWLFKVCKNLWIDELRARAVRV
                     KAAQTRELDEEPTISGEEVVHAELGLRAVERALATLAPEQRAVLTLVAVEGLSYREAA
                     EVLDTPIGTVMSRLARARAALADRLGGAGDGGNHG"
BASE COUNT          737 a         1765 c         1746 g          769 t
ORIGIN      
        1 gatcggcgtc gagcaaaccg tcgaggctcc ggacgcggtc atcgtcgaag gacgctacac
       61 gggcgtctac aagccgaccg gccggcgcat ggacgttcag gtctgccacg tgtggcgttt
      121 ccgcggcgag cgcatcgcga gcttccatca gtacgtcgac acggccgcgc tgcgccgcgt
      181 catggcgcgc gacgacgcgg ctgccgcgtg atgcgagccg agatcagtgc gagcggccgt
      241 cgaagtgctt gaccggtatc ttgtcgagct cgatgccttc gaggcagcga atattgatgg
      301 ccgcgcgcgc gttgcccttc tgatcggtgc cttcgccgaa cggatgaatt ccgcagttcg
      361 agcagaagcg gtgcttgatg acgtgcttgt tgaacgtgta cgtgctcaag ctcgactccg
      421 gcgtcgtgag gcgcaggttg gcgcgcggca cgaaccacag cagcgatccc ttgcgctcgc
      481 acatcgagca gttgcagctc atcgcgctct cgatcgcgcc ctcgacctcg aacgcgacgc
      541 gaccgcagtg gcagctacct ttgtacttca tcgctggcct ttttgagaga aacggcgttg
      601 atacagaacc gcaggccgct cggcggcggg ccgtccggga acacgtggcc caagtgcgcg
      661 tcgcagacgt tgcagagcgc ctcgacgcgc tgcattccgt agctcgtatc gaggtgatag
      721 gcgacgacgc ccacgttcag cggctgcgtg aagctcggcc accccgtgcg gctgtcgaac
      781 ttgctcgtcg agtcgaacag cggcgtgtcg cagcaaacgc agtggtagcg gcccggcgtg
      841 aacagcgcgc acatctcgga gctgaagggc cgctccgtgc ccttcttgcg cgcgacctgg
      901 tattgctcgt cggacaactg cgctcgccac tcggcgtcgc tcttctcgac gcggcgcggc
      961 ggcggctcgt tgcccgagcg cgcgagctcg acgacgtgct tccagcgcag ctccgcgggc
     1021 gacggctctc gtacggcgct catgcgtcag cccgtccgcg ccgcggcgcg ccgcgacagc
     1081 ttccaattcg ggcgcgggaa gtggcatgta tagccgcccg gatagcgcaa gagatagtcc
     1141 tggtgctcgg gctccgcgtc ccaaaacggg cccgcggact tgacctcggt gacgacgcgg
     1201 cccggccata gcttcgacgc gtcgacgtcg gcgatcgtgt cctcggcaat ctcgcgctgc
     1261 tcgtccgaga ggtagtagat gccggagcgg tacgacatcc cgacgtcgtt gccttggcgg
     1321 ttcttcgtcg tcgggtcgtg aatctggaag aagaattcga gcagctctcg gtacgacagc
     1381 cgcttcggat cgaacacgac ctcgatggcc tcggcatgcg tgccgtggtt gcgatacgtc
     1441 gcgtgcggca cgtcgccgcc gctgtagccg acgcgcgtcg agacgacgcc gggcagcttg
     1501 cggatcaggt cctgcatgcc ccagaagcag ccgcccgcga gaattgccgt ttcggtttgg
     1561 ctcatgacga tctcccgtag acaccaagga tatgccttgg agcgggcctt gagccagatc
     1621 gttcgcgagt cagcgcaata cggcgcgcag ctgctcgacg agccagtcga gctcggcgcg
     1681 ctcgacgatc agcggcggcg cgagacggac gacctgctcg tgcgtgtcct tggccagcac
     1741 gccgcgctcg agcagccgct cgcagtacgc gcgcgccgag cccgaagcgg acttgatctc
     1801 gatgcccacg agcaggccgc ggccgcggat ctcggcgatg cacggcgcgc cgagcgcgcg
     1861 cagcttgccg aacaggtatt cgccttgctc ggccgcgcgc tcggcgagcc gctcgcggat
     1921 cagcacgcgc agcgacgcgt gcccgacggc cgccgcgagc ggattgccgc cgaacgtgct
     1981 gccgtggtcg ccgggcttga gcagggtcat gatcgcgcgg ctcgcgagca cggccgagac
     2041 ggggtagacg ccgccgccga gcgccttgcc gagcacgagc atgtcgggct ccgcgcccgg
     2101 ctcgtggtcg tgcgcgaaca tcttgccggt gcgcgcgagg cccgtctgga tctcgtcgag
     2161 cacgaacagc acgttgcgct cgcggcacag cgctcgcgcc gcggcgaggt agccctccgg
     2221 cggcatgatg atgccggcct cggcctgaat cggctcgacg aggaacgcga ccgtattcga
     2281 cgtgatcgcg gcgcgcaacg cggcgagatc gccgaagtcg acgagcgtga agcccggcgt
     2341 gaacggcccg aagtcctcgc ggtattgcgc ctccgacgag aacccgacga tcgtcgtcgt
     2401 gcggccgtgg aagttgttgc ggcagacgac gatctcggcg cggtcgtgcg cgacgccttt
     2461 gacgcggtag ccccacttgc gcgcgagctt gatcgccgtc tcgacggcct cggcgcccgt
     2521 gttcatcggc agcgccatct cgaagcccgc gagcgcgcag agctcgttca gaaacggccc
     2581 catgcgatcg ttgtgcacgg cgcgcgacgt cagcgtcagc tgccccgctt gctcgacgag
     2641 cgccgcgacg atgcgtggat gacggtggcc ctgattcagc gacgagtacg cgctgatgca
     2701 atcgagatag cgccggcctt cgacgtccca tacccagacg ccttcgccgc gggccaagac
     2761 gacgggcagc ggtgaatagt tcggcgcgct gacgcgctcg gcctcggcga tgagcgcgct
     2821 actgtcgctc atagcgtgta cttgtcggcc gcgcgcgcga ggatctgcag cccgaacaag
     2881 ctctggacga gctcgacggc gagctccgcg gtgcgattgc cctcgtcgta cgcggggttc
     2941 agctccatga cgtcgagcga gcgcatgagc cccgagtcgt agatcatctc catgcagagc
     3001 tgcgcttcgc gatacgtcgg cccgcccggg accgtcgacg gcacgccggg cgcgatcgaa
     3061 ggatcgagga agtcgacgtc gaagctgacg tgcaggtgca cgtcgcgctc ggcgagatgc
     3121 gcgagcgcct gctccatgac ggttcgcatg cgcagctcgt cgatacgccg catgtcgtag
     3181 acgaccatgc cgctatcgac gacgagctct ttttcgctcg cgtcgacgga gcggatgccg
     3241 atttgcacga tgcgatccgc ggccagcatc gggtggaagc ccgctaggcc gacgagctcg
     3301 ggcggtccgt gcccggcgat gatgccgacg ggcatgccgt ggatgttgcc cgacggcgac
     3361 gagctcgcga cgttgaagtc ggcgtgcgcg tcgagccaca acatcgcgag cggccgctcg
     3421 cgctccgcgc agtagcgcgc gacggcggcg accgagccga tcgcggcgct atggtcgccg
     3481 cccatcgtca gcggaatctc gcccgcggtc aggctctcgt agacggcgtc gtggacgccg
     3541 cggcaccacg ccgcgacctc gggcaggtgc cggtaatcgc cgcagcgcgg gttctcgggg
     3601 ttgcgcggac ccgtgacgtt gccgcgatcg atgacgccgc gcccgagcct cgacaaggcc
     3661 ttgtcgatgc cggccacgcg tagcgcttcg ggccccatcg aggcgccgcg cttccctgcg
     3721 ccgacgtcgg tcggaacgcc gatcaacgcc acggacgcgg agctcctatc cgccgtcgtc
     3781 atgagccgag tgtggcacgc gggctgcgcc atttgaacct cgcccgttgc agagccctga
     3841 gcgcgggtca gtcgccggcc cagccgcgtc cgacgagcgc gcgctcggcc tccggctcga
     3901 gcggctcgcc gtcgatcaaa gcgtcgacgg cccccgcgac cggcgagtcg ccgcctgccg
     3961 gccgatagac ctcgccgtcc gccgcctcga cgccgacgag gtcgatgcgc caaacgccgc
     4021 cgcggcggca cgcgacggcc gcgttgcgcg acgcgcccga gctgacagcg agctcacggc
     4081 accactcgcc gccttgcgcg cggaacgtgt agcgcgcctc ggccgtcgcg ccgccgacgt
     4141 cgcgcggctc gccgctcggc gaggactcga gcaactcgtg cagcggcgac ccctgctcga
     4201 ccggccccgt cgccgcgagc aagccgctcg ggccgtcgga gcgctggctc aaggtcaggc
     4261 cgagcccgag gccgatcgcg agcgccacgc cggccgcggc ggcatgcggg agccacgcgg
     4321 gtgccacgcg ccgttgccgg cgcgcggcga gatcgacgac cttgccctcg cgcgcttgcg
     4381 gcgcccgcaa cagctcgagc acgcgctcgg gcagcggctc gtcgacgacg tcgcgatacg
     4441 cgtcgccgac cgcgcgattc gcgcgctcca tcgcggcgaa ccgagccgcg aggtcgggct
     4501 cgcgcagcat gcgcgcgcgc agcgccgcgg cttcgtcgct cggcagcgcg ccgtcgatca
     4561 gcgccgagag cgcttcgtcg tcgctcgagg agcttccgtg ctcagccatg attgcctccg
     4621 tcgcccgcgc cgccgagccg gtccgcgagc gccgcgcggg cgcgcgcgag ccggctcatg
     4681 acggtgccga tcggcgtatc gagcacctcg gccgcctcgc gatacgacag tccttcgacg
     4741 gcgacgagcg tgagcacggc gcgctgctcc ggcgccagcg tcgcgagcgc gcgctcgacg
     4801 gcgcggagcc cgagctcggc gtgcacgact tcttcgccgc tgatcgtcgg ctcctcgtcg
     4861 agctcgcgcg tctgcgcggc cttgacgcgg acggcccgcg cgcggagctc gtcgatccag
     4921 aggttcttgc aaaccttgaa cagccaggcc aacggctcga cgccgctcgg ccagccgcgc
     4981 tgaagcgctc gttccaccgt gttttgcatg aggtcgt
//
LOCUS       DASSNN010000603         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_102642, whole genome shotgun sequence.
ACCESSION   DASSNN010000603 DASSNN010000000
VERSION     DASSNN010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102642"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..796
                     /gene="dhaL"
                     /locus_tag="VFL84_11160"
     CDS             134..796
                     /gene="dhaL"
                     /locus_tag="VFL84_11160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HET7609227.1"
                     /translation="MKEITLADVETAVKTIAATALKNEQYFCELDSYAGDADFGVSLA
                     SGFRAVEQEWQALDHSSIGGLLLKISMLISKNVGGCSGPIWGTAFMRAGALSKDRTTI
                     TLDDLDKMLTAAIEGIQKRGGAQLGDKTLLDSLIPIRDLVREHAGKPDADPSEVLRLA
                     TAVANEAIERTKPWVAKRGRQQFTGDRSRDTPDPGIVAIATMMNDLCKQFGVANQAPG
                     KN"
     gene            821..1816
                     /gene="dhaK"
                     /locus_tag="VFL84_11165"
     CDS             821..1816
                     /gene="dhaK"
                     /locus_tag="VFL84_11165"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HET7609228.1"
                     /translation="MKKFVNDPAKFVPEMLEGIFLANEGKLKYEPKYNLIYRADRPNK
                     NHVSVIQGSGSGHEPAHVMTVGPGMLDAASPGNVFAAPPMEYCLECIKLMNTPKGVLV
                     LINNYQGDRMNWDMAIEMAEAEGINVKSFIIDDDVAVQNSSYTVGRRGVAGNFFVMKA
                     CGAAAEQGKSLEQLLEIAKKVNANVRSMGVALTSCTPPAKGKPIFEIGEDEMEVGVGI
                     HGEPGRRRDKMKPADAIVDEIYDAVAKDLPFKSGDRVALMINGLGGTPPSELYVLYRK
                     AALRAKAGGLNIVRNYVGNYCTSLEMAGCSLTLLKLDKEIEELLAAPAEIAVRVF"
     gene            1813..>2141
                     /locus_tag="VFL84_11170"
     CDS             1813..>2141
                     /locus_tag="VFL84_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HET7609229.1"
                     /translation="MSERELKLLALDLDGTLVDSAPDIAHCLGRALEAIGLSAPGEAR
                     TRVWIGDGLETLLKRAIAHGGGEREPTASGKTRHQTALAAFLECYRDNLFVRSRLYPG
                     VKETLAA"
BASE COUNT          425 a          688 c          720 g          308 t
ORIGIN      
        1 aagggcgccg cgaaggtcgt gcccgagctc ggcgacccgc caaaggtgct cgtcacgctc
       61 gaggatttgc aggccatcgg agcacagcgc gcggcgtgac cagttcgtcc gcggcggcaa
      121 ggagaagagt gagatgaagg aaatcacctt ggcggacgtc gaaactgcgg tcaaaaccat
      181 cgccgcgacc gcgctcaaga acgagcagta cttctgcgag ctcgactcgt acgcaggcga
      241 cgctgacttc ggcgtgtcgt tggcttcggg cttccgcgcc gtcgagcagg agtggcaagc
      301 gctcgatcac agctcgatcg gcggcctgct gctcaagatc agcatgctga tctcgaagaa
      361 cgtcggcggc tgctcgggcc cgatctgggg cacggcgttc atgcgcgccg gcgcgctgtc
      421 gaaggatcgc acgacgatca cgctcgacga tctcgacaag atgctgacgg ccgcgatcga
      481 gggcattcaa aagcgcggcg gcgcgcagct cggcgacaag acgctgctcg actcgctgat
      541 cccgattcgc gacctcgtgc gcgagcatgc cggcaagccg gacgcggacc cgagcgaagt
      601 gctgcggctc gcgacggccg tcgcaaacga ggcgatcgag cgcacgaagc cgtgggtcgc
      661 gaagcgcggc cgccagcagt tcacgggcga tcgcagtagg gacacgcccg atccgggcat
      721 cgtcgcaatc gcaacgatga tgaacgacct ttgcaagcag ttcggcgtcg cgaatcaagc
      781 gccgggcaag aactagaaac tcacctcggg ggtacgcacc atgaagaagt tcgtgaacga
      841 cccggccaag ttcgtgccgg agatgctcga aggcatcttt ctcgccaacg aaggcaagct
      901 caagtacgag ccgaagtaca acctcatcta tcgcgccgac cggccgaaca agaaccacgt
      961 ctcggtgatc cagggctcgg gcagcggcca cgagcccgcg cacgtcatga ccgtcggccc
     1021 gggcatgctc gatgccgcgt cgcccggcaa cgtgttcgcc gcgccgccga tggagtactg
     1081 cctcgagtgc atcaagctca tgaacacgcc gaaaggcgtg ctcgtgctga tcaacaacta
     1141 ccagggcgac cggatgaact gggacatggc catcgagatg gccgaggccg agggcatcaa
     1201 cgtcaagtcg ttcatcatcg acgacgacgt cgccgtgcag aacagctcgt acacggtcgg
     1261 ccgccgcggc gtcgccggca acttcttcgt catgaaggcc tgcggcgcag cggccgaaca
     1321 aggcaagagc ctcgagcaac tgctcgagat cgcgaagaag gtcaacgcga acgtgcgctc
     1381 gatgggcgtc gcgctgacgt cgtgcacgcc gcccgcgaag ggcaagccga tcttcgagat
     1441 cggcgaagac gagatggaag tcggcgtcgg catccacggc gagccgggac gccgccgcga
     1501 caagatgaag cctgccgacg cgatcgtcga cgagatctat gacgccgtcg cgaaggactt
     1561 gccgttcaag agcggcgacc gcgtcgcgtt gatgatcaac ggcttgggcg gcacgccgcc
     1621 gagcgagctg tacgtgctgt accgcaaagc ggcgctgcgc gcgaaggccg gcggcctgaa
     1681 catcgtgcgc aactacgtag gcaactactg cacgtcgctc gagatggccg gctgctcgct
     1741 gacgctcttg aagctcgaca aagagatcga agagctgctg gcggcgcccg cggaaatcgc
     1801 cgtgcgagtg ttttgagcga gcgggagctc aagcttctcg ccctggacct agacggcacg
     1861 ctggtcgatt cggcgcccga catcgcgcac tgcctaggcc gtgcgctcga ggcgatcggc
     1921 cttagcgcgc ccggcgaagc gcgcacgcgc gtctggatcg gcgacgggct cgagacgttg
     1981 ctcaagcgcg cgatcgcgca cggcggcggc gagcgcgagc cgaccgcctc gggcaagacg
     2041 cggcaccaaa cggcgctcgc cgcgttcctc gagtgctatc gcgacaacct gttcgtgcgg
     2101 agccgcctct atccgggcgt caaggagacg ctcgccgcgt t
//
LOCUS       DASSNN010000604         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1234140, whole genome shotgun sequence.
ACCESSION   DASSNN010000604 DASSNN010000000
VERSION     DASSNN010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234140"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VFL84_11175"
     CDS             complement(<1..888)
                     /locus_tag="VFL84_11175"
                     /inference="COORDINATES: protein motif:HMM:NF044430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET7609230.1"
                     /translation="MSSTVTISPSSPDAPFASREELEARLASEYGAHLAILDQRLDEA
                     LRACGFEGAVIFAGDEQMIFRDDQAYPFRVEPYFKAWVPLTQAPGSFLRLVPGQRPML
                     VYKQVEDYWHEPPSDPEGYWTQHFDIRVARSDAEARKLSGSGPRWVAIGSAAKRAGRS
                     GSAETQPAVNDARFLSHLDYTRAVKTTYELLCMRGAQAMAVRGHLAVAAAFKSGTSEL
                     QLHQTYLAASGQRETELPYGNIVALNEHAATLHYQRMRADPPPVTRSLLIDAGAEFKG
                     YAADITRTYSASPSDDFAAL"
     gene            1032..1544
                     /locus_tag="VFL84_11180"
     CDS             1032..1544
                     /locus_tag="VFL84_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7609231.1"
                     /translation="MSHAVESLLTQYESGRLRRRDLVALLAALAAAPRTGAAQPAALK
                     AATLNHASLIVSDLDRSVAFYQRTFGLAVKSTQQGGVNLAVGDAFLGIYQGGANAMPH
                     INHFCFGLRGFDPAATVAALEAQGLAAESRTRDGVTQVYTADPDNLRIQLQDVSFCGG
                     VGPLGNECRA"
     gene            1556..>2315
                     /gene="trpS"
                     /locus_tag="VFL84_11185"
     CDS             1556..>2315
                     /gene="trpS"
                     /locus_tag="VFL84_11185"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET7609232.1"
                     /translation="MQVVLTGIKPTGQPGMLHIGNYVGAMLPAVAASKNPSVHSYFFI
                     ADYHAIGGDADKMARSTLEITASWLAVGLDPRKVMLYRQSDIPEIPELTWILHSVTAK
                     GLMNRAHAYKAAVAENEATPGRDPDQGVMMNLYSYPILMAADILMFKAAKVPVGRDQK
                     QHVEMARDIAQRFNHHFGETFVLPEAVIGDNIATLPGLDGRKMSKSYGNVIPLFAGEK
                     ELRKLVMKIKTNSLEPGQPKDPADSALYEIYRAFA"
BASE COUNT          379 a          791 c          789 g          356 t
ORIGIN      
        1 cagcgccgcg aagtcgtcgc tcggcgaagc cgagtacgtg cgcgtgatgt cggcggcata
       61 gcccttgaac tcggcgccgg catcgatcag cagcgaacga gtgacgggcg gcggatcggc
      121 gcgcatgcgc tggtagtgca acgtcgccgc gtgctcgttc agggccacga tgttgccgta
      181 cggcagctcg gtctcgcgct ggccgctggc cgcgagataa gtttgatgca actgcagctc
      241 cgacgtgccc gacttgaacg ccgccgcgac ggcgaggtgc ccgcgcacgg ccatcgcctg
      301 cgcgccgcgc atgcagagca gctcgtaggt cgtcttcacg gcgcgcgtgt agtcgaggtg
      361 gctcaagaag cgtgcgtcgt tgacggccgg ctgtgtctcg gcgctgccgc tgcgtccggc
      421 gcgcttcgcc gccgagccga tcgcgaccca acgcgggccg ctgcccgaga gcttgcgcgc
      481 ctcggcgtcg ctgcgcgcga cgcggatgtc gaagtgctgc gtccaatagc cttcgggatc
      541 gctcggaggc tcgtgccaat agtcctcgac ctgcttgtag acgagcatcg gccgctggcc
      601 cgggacgagg cgcaggaacg accccggcgc ctgcgtcagc ggcacccatg ccttgaaata
      661 cggctcgacg cggaacgggt acgcctggtc gtcgcggaaa atcatctgct cgtcgccggc
      721 gaagatgacg gcgccttcga agccgcacgc gcgcagcgct tcgtcgagcc gctggtcgag
      781 gatcgcgagg tgcgcgccgt actcgctcgc gagcctggcc tcgagctcct cgcgcgaagc
      841 gaacggagcg tcggggctcg aggggctgat cgtgacggtg ctgctcaagt gggtttcgcc
      901 atggcgggcc gttcggctgt tagccgggca tgatagacgg gccccgggcc caagcccaag
      961 cggcgcgggt gcttgaccct cgcgggcacg gcacggagac tgccgttcgc cgtacgcccg
     1021 aggaaagcgc catgagccac gccgtcgagt ccttgctgac ccaatacgag tcggggcgcc
     1081 tccgccgccg cgacctcgtc gcgctgctgg ccgcgctcgc ggccgcgccg cggaccggcg
     1141 ccgcgcagcc cgccgcgctg aaggccgcga cgctgaacca tgcgagcctg atcgtcagcg
     1201 acctcgaccg ctccgtcgcg ttctatcagc gcacgttcgg cctggccgtc aagagcacgc
     1261 agcagggcgg cgtgaatctc gccgtcggcg acgcgtttct cggcatttac cagggcggcg
     1321 cgaacgcgat gccgcacatc aatcatttct gcttcggcct tcgcggcttc gatccggccg
     1381 cgacggtcgc ggcgctcgag gcgcaggggc tcgccgcgga atcgcgcacg cgcgacggcg
     1441 tcacgcaggt ctacacggcc gaccccgaca acctgcggat ccagctccaa gacgtgagct
     1501 tctgcggcgg cgtcggtccg ctcggcaacg agtgccgcgc ttagagaggc cgcgcatgca
     1561 agtcgtcctc accggaatca agccgacggg gcagcccggc atgctgcaca tcggcaacta
     1621 cgtcggcgcg atgctgccgg ccgtcgcggc cagcaagaat ccgagcgtgc actcgtattt
     1681 ttttatcgcc gactaccacg cgatcggcgg cgacgcggac aagatggcgc gctcgacgct
     1741 cgagatcacg gcgagctggc tcgccgtcgg cctcgatccg cgcaaggtca tgctgtatcg
     1801 ccagtcggac atccccgaga ttcccgagct gacgtggatc ctgcacagcg tgacggcgaa
     1861 aggtctcatg aaccgtgcgc acgcgtacaa agccgccgtc gccgagaacg aggcgacgcc
     1921 gggccgcgac cccgatcagg gcgtcatgat gaatttgtac tcgtatccga tcctgatggc
     1981 cgccgacatc ctgatgttca aggcggcgaa ggtgccggtc gggcgcgacc aaaaacagca
     2041 cgtcgagatg gcgcgcgaca tcgcgcagcg cttcaaccac catttcggcg agacgttcgt
     2101 gttgccggag gccgtgatcg gcgacaacat cgcgacgctg cccgggctcg acggccgcaa
     2161 gatgtcgaag agctacggca acgtgattcc gctcttcgcc ggcgagaaag agctgcgcaa
     2221 gctcgtcatg aagatcaaga cgaactcgct cgagccgggt cagccgaagg accccgccga
     2281 cagcgctctc tacgagatct accgcgcgtt cgcga
//
LOCUS       DASSNN010000605         4239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1234320, whole genome shotgun sequence.
ACCESSION   DASSNN010000605 DASSNN010000000
VERSION     DASSNN010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234320"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="VFL84_11190"
     CDS             <1..162
                     /locus_tag="VFL84_11190"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HET7609233.1"
                     /translation="VRVWDSTAEIRYLVIPERPPRTEGMSQEELARLVTRDSMVGAEK
                     LRAPAERRG"
     gene            159..827
                     /gene="nthB"
                     /locus_tag="VFL84_11195"
     CDS             159..827
                     /gene="nthB"
                     /locus_tag="VFL84_11195"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR03888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HET7609234.1"
                     /translation="MNGVHDLGGMDGFPLPARDQGFALHEEWERLTWGMLLATLGIPG
                     IPNGNRRFIESLPPARYLTMPYYARFLESRLKSFLDTGLVTQQELDNPDGPVTMPKLP
                     GFAPRTPEEIVRFLRQEHSGQVNVAVRAAFAAGDEVVVKNDHPAGHTRVPRYVRGCRG
                     VILRDHGVHVFEDDVPAGVRVGPQHLYTVKFTGPELWGSRGHARDRVHVELWEIHLSR
                     ASSP"
     gene            827..1177
                     /locus_tag="VFL84_11200"
     CDS             827..1177
                     /locus_tag="VFL84_11200"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HET7609235.1"
                     /translation="MSQPPKLADLPLLPRDESGPKFAEPWQAQAFAVVIALTESGRIS
                     LKEWADRLGAVLAETEARGDYDTGRRYYDHWLTALERLVVEKNMTDWDELEHEGETIR
                     EDDDHRREEQLGGR"
     gene            1250..1987
                     /locus_tag="VFL84_11205"
     CDS             1250..1987
                     /locus_tag="VFL84_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7609236.1"
                     /translation="MTTASSKAGRRAAVTGAASGIGAATARLLRSQGWRVACLDRNLA
                     GARATAGQDGLAVEVDVADEASVTRAFKDVDAAFGGLDALVTAAGIINTTPFFETTVA
                     EFRKLFDVNVIGSFLTVREGAKYMQKGARICMVASISSYTGGGYVARGAYATSKGAVL
                     TMMKGCARELGPKGIAVNAVAPGFIDTPFVASAMSDPKRRKEVEAAVGKIGTAEQIAE
                     CCAWLVSPAADFVHGETLIADGGILMR"
     gene            complement(2054..3274)
                     /locus_tag="VFL84_11210"
     CDS             complement(2054..3274)
                     /locus_tag="VFL84_11210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET7609237.1"
                     /translation="MSKRPQPARSAPAVKPHSAADRSPDARGPFTPELEAEFGRQQLA
                     ENRTLIRVVALVALLLAAVRGADQILLGSWTPFQLGLFGVVLLASILLAATAWSPWFQ
                     RLYLPCAQVAIPLRGSLIAFVVAGVASRGQLESLMLLPLLMVGPFLVFGLRFRTATIT
                     VALTITVYATAAAIFGLPAPLIVRSCVLLMVVAGACGVVARTLERSARMRFIETHAMA
                     ELAQQDALTGVKNRRVFDEHLETFWQRAIDEERGIAILLIDVDHFKAYNDRYGHQAGD
                     RALRRVAEMIQMFVTRQNDLVARYGGEEFAVLMYDMDGVAAEKVAAQMRKAVCAMGIE
                     HRDSRLGQIVTISIGVAVVDPSPERRARGALQLADEALYQAKTMGRNRVEVMDSAAHK
                     ALETGVFTKGTGLR"
     gene            complement(3384..>4239)
                     /locus_tag="VFL84_11215"
     CDS             complement(3384..>4239)
                     /locus_tag="VFL84_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7609238.1"
                     /translation="PFLRHHFYYLAAVHAFAFYGLWGQILTRGVLAAVDTDAAVVNLV
                     AVLVPVLGVPFLLVSWAMLVNMAGSMFGRGIKPAWHSLHLVVFGLLLLGAWLAVGRLQ
                     AGSASPLDVLGLVEAAALIGIDLVYFAAFLALVWRFRLVVDEDKRRVLLVFSSLLFGA
                     FVARSLLAALVLVDIRLGVVALLAFHGSNLPALLYLRSKADRVFTPVKAEHATKQGME
                     HVLDRYGVTKRERQIVQKICLGKTNKQIAEELFISLQTVKDHTHRIYSKIGVNSRMQL
                     VQMMNTAK"
BASE COUNT          782 a         1394 c         1449 g          614 t
ORIGIN      
        1 gtacgcgtct gggacagcac ggccgagatt cgctatctcg tgattcccga gcgacctccg
       61 cgcaccgaag gcatgtcgca agaagagctc gcgcgcttgg tcacgcggga ctcgatggtc
      121 ggcgccgaga agctgcgtgc gccggcggag aggcgcggat gaacggcgtg cacgatctcg
      181 gcggcatgga tggattcccc ttgcccgctc gcgaccaagg cttcgcgctg cacgaagagt
      241 gggaacgcct gacgtggggc atgctgcttg ccacgctcgg aatcccgggc ataccgaatg
      301 gcaaccgcag gttcatcgag agcctgccgc ccgcgcgtta cctgacgatg ccgtactacg
      361 cgcgcttcct cgaatcgcgc ctgaagagct ttctcgacac cggtctcgtc acgcaacaag
      421 agctcgacaa tccggacggc cccgtcacga tgccgaagct gcccggcttc gcgccgcgga
      481 cgcccgagga gatcgtgcgg ttcttgcgcc aagagcattc gggccaagtg aacgtcgcgg
      541 tgcgcgccgc attcgcggcc ggcgacgagg tcgtcgtcaa gaacgaccac ccggccgggc
      601 acacgcgcgt gccgcgctac gtgcgcggct gccgcggcgt gatcttgcgc gatcacggcg
      661 tgcacgtgtt cgaggacgac gtgccggccg gtgtgcgggt cggcccgcaa catttatata
      721 cggtgaagtt cacggggccg gagctctggg gcagccgcgg ccacgcacgc gaccgcgtgc
      781 acgtcgagct atgggaaatt catctttcgc gcgcctcgag tccctgatga gccaaccgcc
      841 gaagctagcg gacctacctc tactgccacg cgacgagtcg ggtcccaagt tcgcggagcc
      901 gtggcaagcg caagcgttcg ccgtcgtcat cgcgctgacg gaatccggcc gaatctcgct
      961 caaagaatgg gccgatcgcc tgggcgccgt gctcgcggag accgaagcgc gcggcgacta
     1021 cgacacgggc cgccgttact acgaccactg gctcacggcg ctcgagcggc tcgtcgtcga
     1081 gaagaacatg acggactggg acgagctcga gcacgaaggc gaaacgatcc gcgaagacga
     1141 tgaccatcgg cgcgaggagc agctcggagg acgctgagcg accctctcgc agcacgcggc
     1201 aataacgact aggctgcgcg ccatacgacc cggccagagg gcccattcga tgaccacggc
     1261 ttcaagcaaa gcgggccgcc gcgccgccgt caccggcgcg gcgagcggca tcggcgccgc
     1321 gacggcgcgg ctccttcgat cgcagggctg gcgcgtcgcc tgcctcgacc gcaacctcgc
     1381 cggcgcgcgc gcgacggccg ggcaggacgg cctcgccgtc gaggtcgacg tcgccgacga
     1441 agcgtccgtc acgcgtgcgt tcaaagacgt cgacgcggct ttcggcgggc tcgacgcgct
     1501 cgtcacggcg gcgggcatca tcaacacgac gccgttcttc gagacgacgg tcgccgagtt
     1561 tcgcaagctc ttcgacgtca acgtcatcgg ctcgttcctg accgtgcggg aaggcgcgaa
     1621 gtacatgcag aagggcgcgc gcatctgcat ggtcgcgagc atctcgagct acacgggtgg
     1681 cggttacgtc gcgcgcggcg cgtacgcgac gagcaagggt gccgtgctga cgatgatgaa
     1741 gggctgcgcg cgcgagctcg gcccgaaagg catcgccgtg aacgccgtcg cgccgggctt
     1801 catcgatacg ccgttcgtcg cgtcggcgat gagcgatccc aagcgccgca aggaagtcga
     1861 ggcggccgtg ggcaagatcg gcacggccga gcagatcgcg gaatgctgcg cgtggctcgt
     1921 ctcgccggcc gccgacttcg tgcacggcga gacgctgatc gcggacggcg gaatattgat
     1981 gcgctgagcg cgccgcgaag agctcgccgt caccggcgtg atgaacgcgc ccgagcagcg
     2041 ggatgccggt cgtctagcga aggccggtgc ccttcgtgaa cacgccggtc tcgagcgctt
     2101 tgtgcgcggc cgagtccatg acctcgacgc gattgcggcc catcgtcttg gcttgataga
     2161 gcgcctcgtc ggcgagctgg agcgcgccgc gcgcgcggcg ctcggggctc ggatcgacga
     2221 ccgcgacgcc gatgctgatc gtgacgatct gcccgagccg cgagtcgcga tgctcgattc
     2281 ccatcgcgca gacggcctta cgcatctgcg ccgcaacctt ctcggccgcg acgccgtcca
     2341 tgtcgtacat cagcacggcg aactcttcgc cgccgtagcg cgcgaccaga tcgttctgcc
     2401 gcgtcacgaa catttggatc atctctgcga cgcgccgcag cgcgcgatcg ccggcctggt
     2461 ggccgtacct gtcgttgtaa gccttgaagt gatcgacgtc gatcaacagg atcgcgatgc
     2521 cgcgctcctc gtcgatggcg cgctgccaga acgtctcgag atgctcgtcg aagacgcgcc
     2581 ggttcttgac tccggtcaac gcatcctgtt gcgcgagctc ggccatcgcg tgcgtctcga
     2641 tgaaccgcat gcgcgcggaa cgctcgagcg tgcgcgcgac gacgccgcaa gcgccggcga
     2701 cgaccatcaa tagcacgcac gagcgcacga tcagcggcgc cggcaggccg aagatcgcag
     2761 cagcagtcgc gtacacggtg atcgtcaggg ccacggtgat cgtcgcggtc cggaatctca
     2821 ggccgaacac gaggaacggt ccgaccatga gcaacggcaa cagcatcagc gactcgagct
     2881 ggccgcgcga cgcaacgcct gcgaccacga acgcgatcag cgagccgcgc agcggaatcg
     2941 cgacctgcgc gcacggcaag tagagccgct ggaaccacgg gctccacgca gtcgcggcga
     3001 gcaggatcga cgcgaggagc acgacgccaa aaaggccgag ctgaaacggc gtccaagagc
     3061 cgagaaggat ctggtccgcg ccgcgtacgg cggccagcaa cagcgcgacg agcgccacga
     3121 ctcgaatcag cgtcctgttc tcggcgagct gctggcggcc gaactcggcc tcgagctccg
     3181 gcgtgaatgg gccgcgcgcg tcggggctcc ggtccgctgc cgaatggggt ttgaccgccg
     3241 gtgccgaacg ggcgggctga ggtcgcttgg acatgacgtc acgataaggg ccgtgtcggg
     3301 ccgaaaatgt gatgcggtta gcgtattggc ctttggtccg agcgagccgc ggaggtgacg
     3361 ctccgctccg ccggcagcga aatttattta gcagtattca tcatctgcac gagctgcatg
     3421 cggctgttca cgccgatctt cgagtagatg cgatgcgtgt ggtccttgac ggtctgcaag
     3481 ctgatgaaca gctcctcggc gatctgcttg ttcgtcttgc cgaggcagat tttttgcacg
     3541 atctgccgct cgcgcttcgt gacgccgtag cggtcgagca cgtgctccat gccctgcttc
     3601 gtcgcatgct ccgccttgac cggcgtgaac acgcggtcgg ccttcgagcg caggtacagc
     3661 aaggccggca gattggagcc gtggaaggcg agcagtgcga cgacgcccaa gcgaatgtcg
     3721 acgagcacga gcgccgcgag cagcgagcgc gcgacgaacg cgccgaacag cagcgaggag
     3781 aaaacgagca agacacggcg cttgtcctcg tcgacgacga gacggaagcg ccagacgagg
     3841 gcgagaaacg cggcgaagta cacgaggtcg ataccgatca gcgcggccgc ttcgacgaga
     3901 ccgagaacgt cgagcggcga cgccgacccg gcttgcaatc ggccgacggc cagccacgcg
     3961 ccgagcagca acaagccgaa gacgacgagg tgcagcgagt gccaggccgg cttgataccc
     4021 ctgccgaaca tcgagccggc catgttgacg agcatggccc acgagacgag caggaacggc
     4081 acgccgagca cgggcacgag cacggcgacg aggttgacga ccgccgcatc ggtgtcgacg
     4141 gccgccagaa cgccgcgcgt caatatctga ccccacaagc cgtagaacgc gaacgcatgc
     4201 accgcggcga ggtagtagaa gtggtgccga agaaacgga
//
LOCUS       DASSNN010000606         3756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1850786, whole genome shotgun sequence.
ACCESSION   DASSNN010000606 DASSNN010000000
VERSION     DASSNN010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850786"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFL84_11220"
     CDS             complement(<1..693)
                     /locus_tag="VFL84_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609239.1"
                     /translation="MSDSLHSVHWYRVAALRPALRAHLRLIRQAYRGEPWYVIHDPAS
                     GRNWRFSAAAERVIRLMDGRRSVDEIWTLCAQSGGDPPSQDEVVRLLAELHQADALAA
                     DADPDVRELLHRRRDHQARKLRRRFGNPLMLRVRLLDPDAFLTRTLPFVRPLFGAFGA
                     AAWLATVACGLLLCALHWRELGASVADLVFSPQTLLVAFLVYAPIKAVHELAHAYAVK
                     VRGGEVHELGLSW"
     gene            complement(698..2194)
                     /locus_tag="VFL84_11225"
     CDS             complement(698..2194)
                     /locus_tag="VFL84_11225"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET7609240.1"
                     /translation="MASPAARRDPERAETDLGRAETGARAAGGAPSVPTPTRPSTGDA
                     THEALDLLVAALEHRSMQAAATAVVNRLAASVGARRVSLGLWNGSSVELCAVSRSAAF
                     DAATGLSIAVRDAMHEAIAQRATMSVPSDPAASWREDAAQRVLLRLCGGGAVCTVPFA
                     DGTAVGGAVTLEWNDAQTLTSSVRERCEATLALVGPVLGAIARADLGFFRRLRDGLAA
                     ALRAALGPRRPLKKLTLLVAVALVTASSLVTGVHRVSGDAVLRGSVRRIVSAPVDGFI
                     AAAPARPGDVVAEGALLATFDDQALSLEVAKWQAEYEQALNEYREALAQLDSARVTTL
                     RAAMDRALAELELAEDNLARSEIRAPIAGVVVSGDFSQSLGAPLERGATLFELAPLDG
                     YHVTVRVPEQDVGYVAAGQHGRLALAALPGESVELVVERVTPVSEIVDGRNVFEVEAR
                     LLDARDALRPGMQGTAKLDVGNERLLWIFTHDAVDWLRLQLWRLGITG"
     gene            complement(2526..2948)
                     /locus_tag="VFL84_11230"
     CDS             complement(2526..2948)
                     /locus_tag="VFL84_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7609241.1"
                     /translation="MAQTITGGCLCGAVRYKITGDLIFSGKCYCEDCRKSGSTGHSSV
                     LAVPEPSVSMTGKLTEFKKAGSSGQPITRKFCPVCGSRISSSAAVMPGVLMINASSLD
                     DPKVFVNQMSVFASRAPSWDRPPSDAPSFPEMPPPQPH"
     gene            complement(3029..>3756)
                     /locus_tag="VFL84_11235"
     CDS             complement(3029..>3756)
                     /locus_tag="VFL84_11235"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7609242.1"
                     /translation="GRGAASEFAGLEVGQVLHAGTTSWNVVGQFRDRGSVAESEIWTD
                     APVLQGVYNRGTSYQSVRARLTEPQAFQAFKDALTSDPRLNVRVFTERQYYEEQSRTM
                     TAIVGTVGTVIAILMGLGAIFAAVNTMYSAVSARAREIATLRALGFGRFPIVVSVLVE
                     AALIAAVGGVLGMTIAYFAFNGWKASTLNFQSFSQITFAFTVTPQLAVQALVYALTLG
                     LFGGLFPSIRAARQPIVRGLREL"
BASE COUNT          590 a         1346 c         1317 g          503 t
ORIGIN      
        1 ccagctcagg ccgagctcgt gcacctcgcc gccgcgaact ttgacggcat aggcatgggc
       61 gagctcgtgc acggccttga tcggggcgta gacgaggaac gcgacgagca gcgtctgcgg
      121 cgagaagacg aggtccgcga cgctcgcgcc gagctcgcgc cagtgcagcg cgcagagcag
      181 cagcccgcag gctaccgtcg cgagccacgc ggccgctcca aacgcgccga agagcggccg
      241 cacgaacggc aacgtgcgcg tcaggaacgc gtcgggatcg agcagccgca cgcgaagcat
      301 gagcggattg ccgaagcgcc ggcgcagctt ccgcgcctga tgatcgcgcc ggcggtggag
      361 cagctcgcgc acgtcggggt ccgcgtccgc tgcgagcgcg tcggcctggt gcagctcggc
      421 gagcagccgc acgacctcgt cctggctcgg cgggtcgccg cccgactgcg cgcacagcgt
      481 ccagatctcg tcgaccgagc gccgcccgtc catgaggcgg atgacgcgct cggccgccgc
      541 cgagaagcgc cagttgcggc cgctggccgg gtcgtggatc acgtaccacg gctcgccgcg
      601 atacgcctgg cggatcaggc gcaggtgcgc gcgcaacgcc ggccgcagcg ccgcgacgcg
      661 ataccagtgc acgctgtgca gcgagtcgct catggcgtca gcccgtgatc ccgagccgcc
      721 acagctgcag gcgcagccaa tcgacggcgt cgtgcgtgaa gatccagagc agccgctcgt
      781 tgccgacgtc gagcttcgcc gtgccttgca tgccgggccg cagcgcgtcg cgggcgtcga
      841 gcaagcgcgc ctcgacctcg aacacgttgc ggccgtcgac gatctcggag accggagtca
      901 cgcgctcgac gacgagctcg acgctttcgc cgggcagcgc ggcgagcgcg agccgcccgt
      961 gctgcccggc cgcgacgtag ccgacgtctt gctccggaac ccgcacggtc acgtggtagc
     1021 cgtcgagcgg cgcgagctcg aacagcgtcg caccgcgctc gagcggcgcc ccgagcgact
     1081 gactgaagtc gccgctcacg acgacgccgg cgatcggcgc gcgaatttcg ctgcgcgcga
     1141 ggttgtcttc cgcgagctcg agctccgcga gcgcgcggtc catcgccgcg cgcagcgtcg
     1201 tgaccctggc gctgtcgagc tgcgcgagcg cctcgcggta ctcgttcagc gcctgctcgt
     1261 actcggcctg ccacttggcg acctcgagcg acagcgcctg gtcgtcgaac gtcgcgagca
     1321 acgcgccttc ggcgacgacg tcgccgggcc gcgccggagc ggccgcgatg aagccgtcga
     1381 cgggcgccga cacgatgcgc cgcacgctgc cgcgcagcac ggcgtcgccc gacacgcggt
     1441 gcacgcccgt gacgagcgag ctcgccgtca cgagcgccac ggcgacgagc agcgtgagct
     1501 tcttgagcgg ccggcgcggg ccgagcgcgg cacgcagcgc cgccgcgagc ccgtcgcgca
     1561 ggcggcgaaa gaatccgagg tcggctcgag cgatcgcgcc gagcacgggg ccgacgagcg
     1621 cgagcgtcgc ttcgcagcgc tcgcgcacgc tcgaggtgag cgtctgcgcg tcgttccact
     1681 cgagcgtcac ggcaccgccg accgcggtgc catcggcgaa cggcaccgtg cagacggcac
     1741 cgccgccgca gaggcgcagc agcacgcgct gcgcggcgtc ctcgcgccag ctcgcggcgg
     1801 gatcgctcgg gacggacatc gtggctcgct gagcgatcgc ttcgtgcatc gcgtcgcgga
     1861 ccgcgatcga caggcccgtc gccgcgtcga atgccgcgct gcgcgagacc gcgcagagct
     1921 cgacgctcga gccgttccag agacctaagc tgacgcggcg cgcgccgacc gaggccgcga
     1981 ggcggttgac gacggccgtc gcggccgcct gcatggaacg atgctcgagc gccgcgacga
     2041 gcaggtcgag cgcttcgtgc gtggcatcgc cggtcgacgg gcgcgtgggg gtcgggaccg
     2101 aaggggcacc gccagcggcg cgcgcgccgg tctcggctcg gccgagatcg gtctctgcgc
     2161 gttccgggtc gcgtcttgcg gccggacttg ccatggctgc ggtccctcgt cctgaggtca
     2221 actgaggcct gcgagcgccc gtcacacccg ggcaggcctc gtccttcgcg cgcgacgata
     2281 tcgcggccgg accgccgctc gcgttgacta ggctctcata accctctgtt ccgtcagtgc
     2341 gcgcagcccg cgctcgagcc cgaacgtgat gcggctcaca ccgcgcgcgt gacggccgtc
     2401 acggcggcaa gaagctcgcg tgcgaaatac gtgcttgcag aacgcagttg actcgcggtc
     2461 gcgagcgagc gcgtgccgcc gccacggcgg gcggcggcag cgcgaaggaa cggagccggc
     2521 ggccgctaat gtggctgcgg cggcggcatc tccgggaacg acggcgcatc gctcggcggg
     2581 cggtcccagc tcggcgcgcg cgacgcgaac acgctcatct gattgacgaa caccttcggg
     2641 tcgtcgaggc tcgacgcgtt gatcatcagc acgcccggca tgaccgccgc ggagctgctg
     2701 atgcgcgagc cgcacacggg gcagaacttc cgcgtgatcg gctggccgct gctgccggcc
     2761 ttcttgaact ccgtgagctt cccggtcatc gacacggaag gctccggaac cgcgagcacg
     2821 gacgagtgac ccgtgctgcc gctcttgcgg cagtcctcgc aatagcactt gccgctgaat
     2881 atcaggtcgc ccgtgatctt gtagcggacc gcgccgcaga gacagccgcc ggtgattgtc
     2941 tgagccattt tctattctcc ttgtccgaag ccgggttgag cgcccccagc gtaccaaaag
     3001 atgccgtgtc ggaaatcgcc cgcaaaacct acagctcgcg cagccccctc acgatcggct
     3061 ggcgcgctgc gcgaatgctc ggaaacaagc cgccgaaaag ccccagggtc aaggcgtaca
     3121 ccagtgcttg gaccgcaagc tgcggcgtca cggtgaacgc aaacgtgatc tggctgaacg
     3181 actggaagtt cagcgtcgac gccttccagc cgttgaatgc gaaataggcg atcgtcatcc
     3241 cgagcacgcc acccacggcc gcgatcagcg ccgcctcgac gagaacggag acgacgatcg
     3301 ggaaccggcc gaacccgagc gcgcgcagcg tcgcgatctc gcgcgcgcgc gccgagaccg
     3361 ccgagtacat cgtgttgacg gcagcgaaga tcgcgccgag acccatcagg atcgcgatga
     3421 ccgtgccgac cgtcccgacg atcgcggtca tcgtccgcga ttgctcctcg tagtattgac
     3481 gctccgtgaa gacacgcacg ttgagcctcg ggtccgaggt tagcgcgtcc ttgaacgctt
     3541 ggaacgcctg cggctcggta aggcgcgcgc gcacggattg atagctcgtg ccgcggttgt
     3601 agacgccttg aagcaccggc gcgtcggtcc agatctccga ctcggcgacg ctgccgcgat
     3661 cgcggaactg gcccacgacg ttccaagacg tggtgccggc atggagcacc tgcccgacct
     3721 cgagccccgc gaactccgac gcagcgccgc ggccga
//
LOCUS       DASSNN010000607         5519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_233038, whole genome shotgun sequence.
ACCESSION   DASSNN010000607 DASSNN010000000
VERSION     DASSNN010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233038"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VFL84_11240"
     CDS             complement(<1..1080)
                     /locus_tag="VFL84_11240"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HET7609243.1"
                     /translation="MSWAVAASVAPAQPQSTTPAVKDAAVAKAMTAAARALLESVRGE
                     PEFSERLRSYSMEDKLKLTLDDPWREKWQYWPTERSGLSFDLMHAKHRALTHELLWVL
                     LSNRGYHKLLNIMQLENVLQPSSATGFPRGIEEYSVTLFGEPSDTAPWAWRFEGHHVS
                     LSITVAPGQGFTVTPTFFGADPAEVAAGPLAGLRVLRVEEDLGLQLASSFTEAQRRSA
                     LVPGTPEWNKTGGFAYPGDVPWDIVAATILRDRSDWNSWKTELQPDGIAFKDLNGSQQ
                     AALLGILDELFTTYRPEIADVYRAALDLDGLRFAWIGGTKKGEPHYYRIQTRDFLFEL
                     DNVQGNANHIHEVWRSRAGDFGEDLL"
     gene            1297..1743
                     /locus_tag="VFL84_11245"
     CDS             1297..1743
                     /locus_tag="VFL84_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609244.1"
                     /translation="MRCATSWSRPSRIAVAVLVLALSPLAALAQGNGVPRAIGEFSPP
                     TRAQSISAQTGKALNEAISAMNAKRFAAARTAIGELRLNRLSPYERGMVELILFNSYY
                     AESKFAEARPHLVNALESGGLSDEQAAAAQEQIKRIDAWLASAPRA"
     gene            1752..2999
                     /locus_tag="VFL84_11250"
     CDS             1752..2999
                     /locus_tag="VFL84_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609245.1"
                     /translation="MYRSALALAACAIVASAAHAQRDDAIFPGRKGGQDTFGHYAVVA
                     DWPKPLAQLPGHEGWTYGAAQSVFAASPDRIFVLMRGELPALERPATQRLRDVGPSLE
                     YPVFRLPIRSATVASPPAGGAAGAMPPDGLKAWQDAGGVLGVDARWEHCILVFDRNGN
                     IVEDWTQWDSMLARPHAVYVSPYDPDEHVWIVDDFRHAIFKLTNDGKRLVQTIGTVNE
                     HGADATHFFRPTFMAWLPDGTFFVADGYENTRVVKFDASGKYMTAWGEKGTPPNETRP
                     GYMNNVHGIAVDPRTRRVFVNDRANNRAQVFDENGKFLDQWSFGGPPSDIHLFLIDGD
                     GYLWAADRGTNKILKYSLDGKLLYSWGTWGTFPGGMWGVHGMSVDTEGNFYTAEVDTG
                     RVQKFRPMRDANRRYLLAPPRRP"
     gene            complement(3011..3631)
                     /locus_tag="VFL84_11255"
     CDS             complement(3011..3631)
                     /locus_tag="VFL84_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HET7609246.1"
                     /translation="MSLEQISAHIEAHLGKADFVFHELVSDTVHIDIHIVEPRRCFPY
                     IRLVTSGMSDLPMTIPDDADVPRFAELMVTLPETWALNQKSFKDESLYWPLRLLTELA
                     RLPHKHATWLGFGHTVPNGDPPEPYAASTKLCGAIILPSVTVPEEFHTLRIDETKEIA
                     FFSIVPLYADELAFTLREGANELLDKIDSAELGDVIDPLRRSVLDE"
     gene            complement(3825..4106)
                     /locus_tag="VFL84_11260"
     CDS             complement(3825..4106)
                     /locus_tag="VFL84_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609247.1"
                     /translation="MPVLIGCLGLMAPRFAIVLVVIFSDYIGQAYTTVIWPFVGFLFM
                     PLTTLAYAWAINSRGSVAGFHLVVVVLAVLIDLGIVGGSAANRRRRAGA"
     gene            complement(4132..4887)
                     /locus_tag="VFL84_11265"
     CDS             complement(4132..4887)
                     /locus_tag="VFL84_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609248.1"
                     /translation="MGLERIAVAGCVLLVAASAAAQVPTKGEHNADLSGVWTNQNLPG
                     TAEWAINTFSKDLPEMTAWGKARFDAAKPQRGPRGVPVAETDDLVYKCYPPGTPRIYI
                     HPFPMEIIQLPGRVLMIFEYDHLVRQIYTDGREHRTDLPASWMGDSIGHWEGKTLVVE
                     STNFNDKTWIDRIAVPHSEQMKLIERLHLNDQGQLEIDMRIEDPVALAKPWEFSRYYR
                     KTDWTIEELMCEDNATYTPFENALLEFDGDESE"
     gene            complement(4898..5281)
                     /locus_tag="VFL84_11270"
     CDS             complement(4898..5281)
                     /locus_tag="VFL84_11270"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609249.1"
                     /translation="MRSKIGLMLTAAMFAAGPVSAHHGAGVFEPEKKVTVSGTVTDFQ
                     FVNPHVLVYLTVKGGDGKEVQWGGELTSPNRLARDERAVKWHKEILKPGDAITVTGHP
                     ARNGAPMMDILKIVDAHGTVLIDDE"
     gene            complement(5306..>5519)
                     /locus_tag="VFL84_11275"
     CDS             complement(5306..>5519)
                     /locus_tag="VFL84_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET7609250.1"
                     /translation="DGMKVDLAGNVYATGPGGILVIAPDGTHLGTIRPPEALTNVGWG
                     DDGRTLYITGRTALYRIRLSARGATS"
BASE COUNT          955 a         1845 c         1763 g          956 t
ORIGIN      
        1 cagcagatcc tcgccgaagt ccccggcgcg gctgcgccag acctcgtgga tgtggttcgc
       61 gttgccttgc acgttgtcga gctcgaacag gaaatcgcgc gtctggatgc gatagtagtg
      121 cggctcgcct ttcttggtgc cgccgatcca cgcgaaccgc aaaccgtcga ggtcgagcgc
      181 agcgcgatag acgtccgcga tctcgggccg atacgtcgtg aagagctcgt cgaggatgcc
      241 gagcagagcc gcttgctggc tgccgttcaa atccttgaac gcgatgccgt cgggctgcag
      301 ctccgtcttc cacgaattcc aatcgctgcg atcgcgcagg atcgtcgcgg ccacgatgtc
      361 ccagggcacg tcgcccgggt acgcgaagcc gccagtcttg ttccattccg gcgtgcccgg
      421 cacgagcgcg ctgcgccgct gcgcctcggt aaacgagctc gcgagctgca ggcccaagtc
      481 ctcttccacg cgcagcactc gcaggccggc gagcggaccc gcagccactt cggccgggtc
      541 cgcgccgaaa aacgtcggcg tcacggtgaa cccttggccc ggcgcgaccg tgatcgacaa
      601 cgagacatgg tgcccttcga agcgccacgc ccacggcgcc gtgtcgctcg gctcgccgaa
      661 caacgtgacg ctgtactcct cgatgccgcg cgggaaaccc gtggcggagc tcggctgcag
      721 cacgttctcg agctgcatga tgttcaagag cttgtgatag ccgcgattgc tgagaagcac
      781 ccacagcagc tcgtgcgtca gcgcgcgatg cttcgcgtgc atgaggtcga acgagagtcc
      841 cgatcgctcc gtgggccagt actgccattt ctcgcgccac ggatcgtcga gcgtgagctt
      901 gagcttgtcc tccatcgagt acgagcgcag gcgctccgag aactccggct cgccgcgcac
      961 ggactcgagc agcgcgcggg ccgcggccgt catcgctttg gcgacggccg cgtccttgac
     1021 tgcgggcgtc gtcgactgcg gctgagccgg agcgacgctc gcggcgaccg cccacgacac
     1081 cgcgacgacc tgaagaacag cgagccgcgc tcgcgcactc cgacccttct tgttcacaac
     1141 ggctccgcgt cagcccgcga cggccatacg atggcttggg gcacagccgg tgacaagagg
     1201 taacgcgtgc cgaggccgcc gcatcggccg cgtgctatgg taaccgcggt tccgagtgct
     1261 cgacgacaac gtccggtcca tcagggggag atcatcatgc gctgcgcaac gagctggtcc
     1321 cgtccttcgc gaatcgccgt cgccgtgctc gtgctcgcgc tttcaccgct agccgcgctc
     1381 gcacaaggca acggagtgcc gcgcgcaata ggcgaattct cgccgccgac acgcgcgcag
     1441 tcgataagcg cgcaaaccgg caaagccctc aacgaggcca tctcggcgat gaacgccaag
     1501 cgcttcgctg ccgcgcgcac agccatcggc gaattgcggc tcaaccgctt gagcccttac
     1561 gagcgcggca tggtcgagct gattctcttc aatagctact acgccgaaag caaattcgcc
     1621 gaggcgcgcc cgcacctcgt caacgctctc gaatcgggcg ggttgagcga cgagcaagcc
     1681 gccgcggcgc aagaacaaat caaacgcatc gacgcatggc ttgcgagcgc tccccgcgcg
     1741 tagcggcagg aatgtataga tcggccttgg cgctcgccgc gtgcgcgatt gtcgcgagcg
     1801 cggcgcacgc gcaacgcgac gatgcgatct tccccgggcg taaaggcggc caggacacgt
     1861 tcggtcatta cgccgtcgtc gccgattggc cgaagccgct cgcgcaattg cccggccacg
     1921 agggttggac ttacggcgcg gcgcaaagcg tgttcgcggc gagccccgac cgcatcttcg
     1981 tgctgatgcg cggcgagctg ccggcgctcg aacgccccgc gacgcagcgg ttgcgcgacg
     2041 tcggcccgag cctcgagtac cccgtgttcc gcctgccgat ccgcagcgcg acggtcgcga
     2101 gcccgccggc cggcggcgct gccggcgcga tgccgccgga tggcttgaag gcgtggcaag
     2161 acgcgggcgg cgtgctcggc gtcgacgcgc gctgggagca ttgcatcctc gtcttcgacc
     2221 gcaacggcaa catcgtcgag gattggactc agtgggactc gatgctcgcg cggccgcacg
     2281 ccgtctacgt gagcccgtac gaccccgacg agcatgtttg gatcgtcgac gacttccgcc
     2341 acgcgatctt caagctcacg aacgacggca agcgcctcgt gcaaacgatc ggcaccgtca
     2401 acgagcacgg cgcggacgcg acgcatttct ttcgcccaac gttcatggcg tggctgcccg
     2461 acggcacgtt cttcgtcgcc gacggttacg agaacacgcg cgtggtgaaa ttcgacgctt
     2521 cgggcaagta catgacggca tggggcgaga aaggcacgcc gccgaacgag acgcgccccg
     2581 gctacatgaa caacgtgcac gggatcgccg tcgatccgcg cacgcggcgc gtgttcgtca
     2641 acgaccgcgc caacaatcgc gcgcaggtct tcgacgagaa cggcaagttc ctcgaccagt
     2701 ggagcttcgg cggtccgccg tccgacattc acttgttctt gatcgacggc gacggatatc
     2761 tttgggccgc cgaccgcggc acgaacaaga tcttgaagta ctcgctcgac ggcaagctgc
     2821 tctactcctg gggcacgtgg ggcacgttcc ctggcggcat gtggggcgtg cacggcatga
     2881 gcgtcgacac cgagggcaac ttctatacgg ccgaggtcga cacgggccgc gtgcagaaat
     2941 tcaggccgat gcgcgacgcg aatcggcgat atctcctcgc cccgccgaga cgcccctaga
     3001 agattccgga tcactcgtcg agcacgctac gccgcagcgg atcgatgaca tcgccgagct
     3061 cggcgctatc gatcttgtcg agcagctcgt ttgcgccttc gcgaagggtg aaagccagct
     3121 cgtcggcgta cagaggaacg atcgagaaga acgcgatctc cttcgtctca tcgatgcgca
     3181 gagtgtggaa ttcttccggc accgtcacgg aaggcaagat gatcgcgccg cacagcttcg
     3241 tgcttgcggc atacggctcc ggcggatcac cattgggcac ggtgtgccca aagcccagcc
     3301 acgtcgcgtg cttgtgcggc aaacgagcca gctccgtgag cagccggagt ggccagtaca
     3361 agctctcgtc cttgaacgac ttctgattga gcgcccaagt ttccggcagc gtgaccatga
     3421 gctctgcgaa ccgaggcacg tcggcgtcgt ccggaatcgt cattggcagg tcgctcatcc
     3481 ccgacgtgac gagcctgatg tagggaaagc acctccgagg ttcgacgatg tggatgtcga
     3541 tgtgaacggt atccgaaacg agctcgtgaa agacgaagtc ggcctttccg aggtgcgcct
     3601 cgatgtgcgc ggagatctgc tcgagcgaca tctctccgtg gggggcttgc cgcggcgcag
     3661 ccggtccatg ccggagaatc ggagtaccgc ccatagacac gagggtcggc ttgtctttgc
     3721 gggagctcat ggtcgcccac tagaccggct tgcgcgtccg ccggcaagcc gattaatcgg
     3781 gcaattggcg agattctgcg tcgagccagt ccggcccgtc ggccttacgc gcctgcgcgg
     3841 cgtcttcggt tcgcggccga tccacccacg atgccgaggt cgatgagaac ggcaagcacg
     3901 acgacgacga ggtggaaacc cgcgaccgat ccgcggctgt tgattgccca cgcgtaggcg
     3961 agcgtcgtca acggcatgaa caagaacccg acgaacggcc agatgacggt cgtgtacgcc
     4021 tgaccgatgt aatcgctgaa gatgacgacg agcacgatgg cgaatcgcgg cgccatcaga
     4081 cctaagcaac cgatcaagac tggcatgctc gcgcctacgc ctccgcctga gctactcgga
     4141 ctcgtcgccg tcgaactcga gcaaggcgtt ctcgaacggc gtgtacgtcg cgttgtcctc
     4201 gcacatgagc tcttcgatcg tccagtccgt cttgcggtag taacgcgaga attcccacgg
     4261 ctttgcgagc gcgacggggt cctcgatgcg catgtcgatc tcaagctggc cttggtcgtt
     4321 caagtgcagc cgctcgatga gcttcatctg ctcgctgtgc ggcacggcga tgcggtcgat
     4381 ccaggtcttg tcgttgaagt tcgtcgactc gacgacgagc gtcttgccct cccaatggcc
     4441 gatcgaatcg cccatccacg acgccggcaa gtcggtgcga tgctcgcggc cgtcggtgta
     4501 gatctgccgc acgaggtggt cgtactcgaa gatcatcagc acacggcccg gcagctggat
     4561 gatctccatc gggaacggat ggatgtagat gcgcggcgtg ccgggcgggt agcacttgta
     4621 gacgagatcg tcggtctcgg cgacagggac gccgcgcggc ccgcgctgcg gcttcgcggc
     4681 gtcgaagcgc gctttacccc aggccgtcat ctcgggcaga tccttgctga acgtgttgat
     4741 cgcccactcg gccgtgcccg gcaagttctg gttcgtccag acgccgctca ggtcggcgtt
     4801 gtgctcgccc ttcgtcggca cttgtgccgc agccgaggcc gcgacgagca agacgcagcc
     4861 cgcgaccgca attcgttcga gacccatgtc gttctctcta ttcgtcgtcg atcagcacgg
     4921 tgccgtgcgc gtcgacgatc ttgaggatgt ccatcatcgg cgcgccgttg cgcgcgggat
     4981 gacccgtcac cgtgatcgcg tcgccgggct tcagtatttc cttgtgccac ttcacggcgc
     5041 gctcgtcgcg cgcgagccta ttgggactcg taagctcgcc gccccactgc acctccttgc
     5101 cgtcgccgcc cttgaccgtc aggtagacga gcacgtgcgg attgacgaac tgaaagtccg
     5161 tcaccgtgcc ggacaccgtg actttcttct cgggctcgaa cacgccggcg ccgtggtgcg
     5221 ccgacacggg cccggccgcg aacattgcgg ccgtgagcat gagaccgatt ttgctgcgca
     5281 tacttatctc cttgcctcgc gcgcgtcagc tggtcgcgcc gcgcgcgctc aaacgaattc
     5341 gatacaacgc cgtgcgcccc gtgatgtaca gcgtacgccc gtcgtcgccc cagcccacgt
     5401 tcgtcagcgc ctccggcggg cggatcgtgc ccaggtgcgt cccgtcgggc gctatgacga
     5461 ggatcccgcc cggccccgtc gcatagacgt tgccggcgag atcgaccttc atgccgtcg
//
LOCUS       DASSNN010000608         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1234398, whole genome shotgun sequence.
ACCESSION   DASSNN010000608 DASSNN010000000
VERSION     DASSNN010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234398"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VFL84_11280"
     CDS             complement(<1..929)
                     /locus_tag="VFL84_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609251.1"
                     /translation="MSASFWVVAGLFCAFAFFLLLVPLWRYRSRAGTWPLAGLVAAAL
                     VVPAAVALYFHVSDWDPEYAAELARNNSIIDEFARRLESTPNDVKGLRLLASSYASLQ
                     RWPEARAVYERLWALTPQPDDELKLAYAEAQLRTDSSSLTGEGGRLVEEVLASNPDNP
                     LALWYGGFVALERGMTDVVRTRWTRLLAFDPDPEFAAMLRARLEELDEAQAQAPVAPV
                     GPEIKVTVTLGEGRAVSALGPNAQLFIIARAPEGGPPIAVIRQPPTAVPGEFSLSDAN
                     AMIAGRSLSAYPEITVIARLSRTGQPTAQPGDW"
     gene            complement(926..1345)
                     /locus_tag="VFL84_11285"
     CDS             complement(926..1345)
                     /locus_tag="VFL84_11285"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HET7609252.1"
                     /translation="MRKLTWLLALWLGLVGSAWAVDDEVAFDDPALNARYHALTREIR
                     CPLCLNESIAESKSDVAQDLRREVRRMIGEGASDDEIKSALAARYGEFILYDPRLSRS
                     TLALWVGPFLFLAVGAFVFWRVLAKRRDQPFDEDPSL"
     gene            complement(1345..1908)
                     /locus_tag="VFL84_11290"
     CDS             complement(1345..1908)
                     /locus_tag="VFL84_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HET7609253.1"
                     /translation="MWRYVVPLAIFVVIGVFFAVGLRLNPSYVPSPLVGKPIPTFSLP
                     AVTDPDAIVSSDMLKGRVSLVNVWGTWCVECGHEHAYLVQLARAGVPIYGLNVHDEER
                     SAAVDWLERLGNPYVISGYDPDGKAAFDWGVTGAPETFLIGSDGTILHKHISPLTPRV
                     WQNDFVPLLRQHCGPGDCAFLAATGGR"
     gene            complement(1911..>2221)
                     /locus_tag="VFL84_11295"
     CDS             complement(1911..>2221)
                     /locus_tag="VFL84_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HET7609254.1"
                     /translation="RSGGDLVTVLRPQIRTYQVQRAPMTEAAIDTNVLRDVFVALGEP
                     LGDNAWSVRMQVKPLIGFLWLGSGLMVLGGLFAITDRRYRAAVRQTDRVAAQPAPGTA
                     "
BASE COUNT          392 a          768 c          758 g          303 t
ORIGIN      
        1 tcccaatcgc cgggctgcgc cgtcggctgg cccgtccgcg aaaggcgcgc gatcacggtg
       61 atctcgggat acgcggagag cgagcgtccc gcgatcatgg cgtttgcgtc gctcaacgag
      121 aactcgcccg ggacggccgt cggcggctgg cggatcacgg cgatcggcgg gccgccttcc
      181 ggcgcgcgcg cgatgatgaa gagctgcgca ttgggcccga gcgccgaaac ggcccgaccc
      241 tcgccgagcg tgacggtgac tttgatctcg gggccgaccg gcgcgaccgg cgcctgcgcc
      301 tgcgcctcgt cgagctcctc gagccgcgcg cgcagcatcg cggcaaactc cgggtccggg
      361 tcgaaggcca acaagcgcgt ccaccgcgtt cggacgacgt ccgtcatccc gcgctcgagc
      421 gcgacgaaac cgccgtacca gagcgcgagc gggttgtccg ggttcgacgc gagcacttcc
      481 tcgacgaggc gcccgccctc gcctgtcagg ctcgaggaat cggtgcggag ctgggcctcg
      541 gcataggcca gcttcagctc gtcgtcgggc tgcggcgtca acgcccacag ccgctcgtac
      601 acggcgcgcg cctcgggcca acgctgcagc gacgcgtagc tgctcgcgag caaccgcaga
      661 cccttgacgt cgttcggcgt gctctcgagg cggcgcgcga actcgtcgat gatcgagttg
      721 ttgcgcgcga gctccgccgc gtactccggg tcccagtcgc tgacgtggaa atagagcgcc
      781 acggcggccg gcacgacgag cgcggcggcg acgagccccg cgaggggcca cgtgcccgcg
      841 cggctcctgt aacgccagag cggcacgagc aagaggaaga atgcgaaggc gcagaagagc
      901 cccgcgacga cccagaaact tgcgctcaca gcgaaggatc ctcgtcgaac ggttgatcgc
      961 gccgcttcgc gaggacgcgc cagaacacga aggcgccgac ggcgaggaac aggaagggtc
     1021 cgacccagag cgcgagagtc gaccgcgaca ggcgcgggtc gtagaggatg aactcgccgt
     1081 agcgcgccgc gagcgccgac ttgatctcgt cgtcggacgc gccctcgccg atcatgcgcc
     1141 gcacttcgcg gcgcaaatct tgcgcaacgt cggacttcga ctccgcgatg ctctcgttca
     1201 ggcacaacgg gcagcggatc tcgcgagtca gcgcgtgata acgcgcgttc agcgcgggat
     1261 cgtcgaacgc gacctcgtcg tcgaccgccc aagcgctgcc gacgaggccg agccacagcg
     1321 cgagcagcca cgtcagcttt ctcatcatcg accgccggtg gccgcgagaa atgcgcagtc
     1381 gccgggaccg caatgttgcc gcagcagcgg cacgaaatcg ttctgccaca cgcgcggcgt
     1441 gagcggcgag atgtgcttgt gcagaatcgt gccgtcgctg ccgatcagga acgtctcggg
     1501 cgcgcccgtc acgccccaat cgaaagccgc tttgccgtcg ggatcgtagc ccgagatcac
     1561 gtaaggattg cccaagcgtt cgagccagtc gacggccgcc gagcgttcct cgtcgtggac
     1621 gttcaagccg tagatcggca cgccggcgcg cgcgagctgc acgagatagg catgctcgtg
     1681 gccgcattcg acgcaccacg ttccccatac gttgacgagc gagactcggc ctttgagcat
     1741 gtccgagctc acgatcgcgt cgggatccgt gacggccggc agcgagaacg tcggtatcgg
     1801 cttgccgacc agcggcgacg gaacgtagct cgggttgagc ctgagaccca cggcaaagaa
     1861 cacgccgatg acgacgaaga tcgcgagcgg aacgacgtag cgccacatcg tcaagcagtc
     1921 cccggcgccg gctgcgccgc gacgcgatcc gtctgccgca cggccgcgcg gtaacggcgg
     1981 tccgtgatcg cgaacaggcc gccgagcacc atgagaccgg agccgagcca caagaaaccg
     2041 atcagcggct tcacctgcat gcgcacgctc cacgcgttgt cgccgagcgg ctcgccgagc
     2101 gctacgaaga cgtcgcgcag cacgttggtg tcgatcgcgg cctcggtcat cggcgcgcgc
     2161 tgcacttgat acgtgcggat ctgcggacgc aaaaccgtga cgagatcgcc gccgctgcgc
     2221 a
//
LOCUS       DASSNN010000609         3800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1234404, whole genome shotgun sequence.
ACCESSION   DASSNN010000609 DASSNN010000000
VERSION     DASSNN010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234404"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1384)
                     /locus_tag="VFL84_11300"
     CDS             complement(173..1384)
                     /locus_tag="VFL84_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7609255.1"
                     /translation="MLFGEIVIVALQSIRANLFRAILTMLGIIIGVGAVITMLAAGAG
                     AQKRIDDQIAALGSNILTVNAAQFFSGGISRDRQTLVVDDVDALIADNRYLDAVVPEM
                     QNRGQLKLNNMNTNTRLVGTTADYATVFNYKLAYGRFFTPEEDELRTRVVVLGADIPG
                     RLEAEPDKLVGRSISVNGQPFEIIGIFESIGGGFGNQSPDASAVIPFRTAEQRVFGRK
                     QMDNISVRVAEDASMERAMVDIERVLRREHKLTPGSANDFSIVDRREFLNTQQEAQQT
                     FTMLLASIAGVSLLVGGIGIMNIMLVSVTERTREIGIRMALGATRFSILLQFLVESVT
                     LCLLGGGLGILLGTVAARTLSRFAGWEVFVSPESVGLASAFSVGVGLFFGIWPARRAA
                     RLDPIEALRYE"
     gene            complement(1441..1671)
                     /locus_tag="VFL84_11305"
     CDS             complement(1441..1671)
                     /locus_tag="VFL84_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609256.1"
                     /translation="MAIEPMLGAAEINGRSVSAAPEHTANNARNERQREAPRSALRKT
                     LCRTPLVIETMVRGTEKRCKSAQPAEKFSAAA"
     gene            1670..2512
                     /locus_tag="VFL84_11310"
     CDS             1670..2512
                     /locus_tag="VFL84_11310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7609257.1"
                     /translation="MLASAPRYPHQAYGYDLALARLQRDAAARSWVAAADRALLQPQA
                     IRLPFGKSGEQASGETANGYAFSVPVGRRITVNVSADGVSPDELFVDVFRAGAGAQRE
                     RVAGTIPGGGAVPVVVEVVDGGDYVLRVQPVLGAAARYDVAIESRPLLAFPVRGVDGR
                     AIWSGFGAERDGGRRAHRGVDIFAPRGTPVLAATDGFVTRVETTRVGGNVIWMQPLFG
                     NLRLYYAHLNEQWVEPGDFVLTGQPLGAVGNTGNAVTTPPHLHFGVYVRRRGGAQDPA
                     EFLR"
     gene            2565..2798
                     /locus_tag="VFL84_11315"
     CDS             2565..2798
                     /locus_tag="VFL84_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609258.1"
                     /translation="MSLRDQVDRLLPGWRTWYPSLFDAAADLGLIRARVCPPGSLLLS
                     RRHADVQRAAEEMHRERWGGGPPEDVAERRDGD"
     gene            complement(2815..3693)
                     /locus_tag="VFL84_11320"
     CDS             complement(2815..3693)
                     /locus_tag="VFL84_11320"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7609259.1"
                     /translation="MIIRSKLIPTAVLSLGLALWSEAGSSAPSHADVAYATSGAGTPL
                     KLDLYLPRAAGAPLLVWLHGGAWENGNKSAMPLAPLVERGFAVASLDFSPASKARFPG
                     QVHEIKAGIRFLRAEASRYGYDASRIGILGASSGAHLAALVGTSNGNAELEGALGDHR
                     DQSSDVHAIVAYFAATNLTTILAQSTPFGLNIREPALQRLLGAAPKEAEALAKLASPV
                     FQVDRGDPPLLLLHGDLDPQMPINQSHELEGAYERQGLEVDFIVVHGAAHGGEAFYSP
                     QNVERVAAFLGATLHP"
BASE COUNT          613 a         1265 c         1294 g          628 t
ORIGIN      
        1 cagctgcacg ggcgtgcgct cgaaggactc gaacggcacg accgcgggtt gcggctcgta
       61 gcggaacccg ggcctcggct cgggatcgcg atggatgtgg aacgcgcagc ccgtgcacgc
      121 gagcgcgaac gacgcgacga gccagctgcg gccgcgcacg agcggcgccg tgttattcgt
      181 agcgcagcgc ttcgatcggg tcgagacgag ccgcgcgacg cgccggccaa atgccgaaga
      241 aaagcccgac gccgacgctg aaagccgaag ccaagcccac cgactccggc gagacgaaga
      301 cttcccagcc tgcgaaccgc gacagcgttc gcgcagcgac cgtgccaagc aagatcccca
      361 atccgccgcc caacaaacac aaagtgacgg actcgaccag gaactgcaag agaatgctga
      421 agcgagtcgc gccgagcgcc atgcggatgc cgatctcgcg cgttcgctcc gtgacgctga
      481 caagcatgat gttcatgatg ccgatgccgc ctacaagcaa gctgacgccg gcgatgctcg
      541 cgagcagcat cgtgaacgtc tgctgcgcct cttgctgcgt gttcaggaac tcgcggcggt
      601 cgacgatcga gaagtcgttc gcgctgccgg gcgtgagctt gtgctctctg cgcagcacgc
      661 gctcgatgtc gaccatcgcg cgctccatcg acgcgtcctc ggcgacgcgc acgctgatgt
      721 tgtccatctg cttgcggccg aagacgcgct gctcggccgt tcggaacggg atcacggccg
      781 acgcgtcggg gctctggttg ccgaagccgc cgccgatgct ctcgaagatg ccgatgatct
      841 cgaacggctg gccgttgacg gagatcgagc ggccgaccag cttgtcgggt tcggcctcga
      901 gccgccctgg aatgtcggcg ccgagaacga cgacgcgcgt gcgcagctcg tcctcttcgg
      961 gcgtgaaaaa ccggccgtac gcaagcttgt agttgaatac ggtcgcgtag tcggccgtcg
     1021 tgccgaccag ccgcgtattc gtgttcatgt tgttgagctt gagctggccg cggttctgca
     1081 tctcgggcac gacggcatcg agataccggt tgtcggcgat cagcgcatcg acgtcgtcga
     1141 cgacgagcgt ctggcgatcg cgagaaatac cgcccgagaa gaactgggcc gcgttgacgg
     1201 tcaggatgtt cgagccgagc gcggcgatct ggtcgtcgat acgcttctgg gcgccggcgc
     1261 ctgccgccag catcgtgatg acggcgccta cgccgatgat gatgccgagc atcgtcagga
     1321 tcgcgcggaa tagattcgcg cgaatcgact gcagcgcaac gatgacgatc tcgccaaata
     1381 gcatgaggta ctactgcttg ccgaacgctg cgcctaacct aggcagcctc cacaataggg
     1441 tcaagccgca gcgctgaatt tttctgcagg ctgcgctgat ttacagcgct tctccgtccc
     1501 gcgtaccatc gtttcgatga ccagcggggt tcggcagagc gttttccgca acgcgctacg
     1561 cggcgcttcc cgctgccgct cgttccgcgc gttgtttgcc gtgtgctcgg gcgccgcgct
     1621 cacgctcctg ccattgatct cggcggcgcc caacatgggc tcgatcgcca tgctcgcgtc
     1681 cgcgccgcgg tatccgcacc aagcgtacgg ctacgatctc gcgctggcgc gattgcagcg
     1741 cgatgcagcg gcgagaagtt gggtcgctgc ggccgaccgt gcgcttctgc aaccgcaagc
     1801 gatccggttg ccgttcggga aaagcggcga gcaggcgagc ggcgagacgg cgaacggcta
     1861 cgcgttcagc gtgcctgtcg gccggcgcat caccgtgaac gtctcggccg acggcgtctc
     1921 gccggacgag ctcttcgtcg acgtatttcg cgccggcgcc ggggcgcagc gcgagcgcgt
     1981 ggccgggacg attcccggcg gcggcgcggt gccagtcgtc gtcgaggtcg tggacggcgg
     2041 cgactacgtt ttgcgcgtcc agccggtgct cggagcggcg gcgcgttacg acgtcgcgat
     2101 cgagtcgagg ccgttgctcg cgttccccgt gcggggcgtg gacggccgcg cgatctggag
     2161 tgggttcggt gcggagcggg acggcggccg acgagcgcat cgtggcgtcg acatttttgc
     2221 gccgcgcggc acaccggtcc tcgccgcgac ggacggtttc gtcacgcgcg tcgagacgac
     2281 tcgcgtgggc ggcaatgtga tctggatgca gccgctgttc ggcaacctgc gcctttacta
     2341 cgcgcatctg aacgagcaat gggtcgagcc cggcgacttc gtgctcacgg gtcagccgct
     2401 cggcgccgtg ggcaacacgg gcaacgccgt cacgacgccg ccgcatctgc atttcggcgt
     2461 ctacgtaagg cgccgcggcg gcgcgcaaga tcccgccgaa ttcttgcgct aacgagaact
     2521 ccgttcgttg ccacctcggc cgaggtccgc taggctccgg ccccatgagc ctgcgcgatc
     2581 aagtcgatcg gctattgccc ggctggcgga cgtggtatcc gagcctgttc gacgccgcag
     2641 ccgacctcgg cttgattcgc gcgcgcgttt gcccgccggg cagcttgctc ttgagtcgac
     2701 gccacgccga cgttcagcgc gccgccgagg aaatgcaccg cgagcgctgg ggcggcgggc
     2761 cgcccgagga cgttgccgag cgtcgcgacg gcgactagcc ttttctcacc ctcgctacgg
     2821 gtgcaacgtc gcgcccaaga acgcggccac gcgctcgacg ttctgcggcg agtagaacgc
     2881 ctcgccgcca tgcgccgcgc cgtgcacgac gatgaaatcg acttcgaggc cctgccgctc
     2941 gtatgcgcct tcgagctcgt gcgattggtt gatcggcatc tgcgggtcca gatcgccgtg
     3001 caggagcagc aacggcggat cgccgcggtc gacctggaac accgggctcg cgagcttcgc
     3061 gagcgcctcg gcctctttcg gcgcggcgcc gagcaaccgc tgcagcgcgg gctcgcggat
     3121 gttcagtccg aacggcgtcg actgcgcgag aatcgtcgtg agattcgtcg cggcgaagta
     3181 cgccacgatc gcgtggacgt ccgaggactg gtcgcgatga tcgccgagcg cgccttcgag
     3241 ctccgcgttg ccgttgctcg tgccgacgag ggcggccagg tgggcccccg aggacgcgcc
     3301 gaggattccg atccggctcg cgtcgtagcc gtagcgcgac gcctcggcac gcaggaagcg
     3361 aatcccggcc ttgatctcgt ggacctgccc cgggaaccga gccttcgagg ccgggctgaa
     3421 atcgaggctc gcgacggcga acccgcgttc gacgagcggt gccagcggca tcgccgactt
     3481 gttgccgttc tcccaggcgc cgccgtggag ccagacgagg agcggcgcgc ccgcggcccg
     3541 cggcaagtac aaatcgagct tgagcggcgt acccgcgccg ctcgtcgcat acgcgacatc
     3601 ggcgtgggaa ggcgcgctcg agcccgcttc ggaccagagt gccagcccga gcgaaagcac
     3661 ggcagttgga attagcttcg aacgaataat catggcgatc cccccatcgt ccgaaggaaa
     3721 ctatatgtca tggcggcggc tgtcgcgcgt accgagcggt ctcgccgccc tggctgtgca
     3781 cagcccgatt ccaacatttg
//
LOCUS       DASSNN010000610         4327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_607619, whole genome shotgun sequence.
ACCESSION   DASSNN010000610 DASSNN010000000
VERSION     DASSNN010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607619"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VFL84_11325"
     CDS             <1..376
                     /locus_tag="VFL84_11325"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7609260.1"
                     /translation="RRAHAAMPRVLELAKRFYDAGVRLSIGTDGTGGGPSFVRELRLH
                     VQAGIPPWDVLRLATSDAAERLGMGELRGALAPGFEADIVFLNSNPLESIENAGDVDT
                     VVQDGRAYRAKELIEMASAPAN"
     gene            469..1005
                     /locus_tag="VFL84_11330"
     CDS             469..1005
                     /locus_tag="VFL84_11330"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET7609261.1"
                     /translation="MTATAAKPETVRLSREELRQRWRQLADNPLVAALSYKVELNEKG
                     AIVVSPPTVRHAFIQGFLTRELARQRPEGTTLPECPVETEIGMRVPDVVWASPDFMKQ
                     HRNEKELSAAPELCIEVLSPTNTRAEIAEKIAAYLSAGAKEVWVVGDDGAAEIHVSAG
                     RVAATTLRFDLAALRRVD"
     gene            complement(1002..1646)
                     /locus_tag="VFL84_11335"
     CDS             complement(1002..1646)
                     /locus_tag="VFL84_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7609262.1"
                     /translation="MSRALGWSAAVALFGVTPVAGANEALLAAATRGDVAAVRALAES
                     GVDLNYTKTAGGETALVVAAQRGYLAVVQELLTRGAAVNTPRADGQTALHLAAANGHL
                     EIATALVEAKADFDFQARPGWTPLMLASAKGHKAIVDMLVAAGAAIDRKTNFGETAFF
                     MAAANGHTDVVKTLLALGANVNDTRGDGSSALRLASERRHVATVQVLLAAGAVD"
     gene            1746..2822
                     /locus_tag="VFL84_11340"
     CDS             1746..2822
                     /locus_tag="VFL84_11340"
                     /inference="COORDINATES: protein motif:HMM:NF023380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7609263.1"
                     /translation="MADHDDKEDGLTAAECAARTGLTVRALRVYEDYGLLAPKRTAGG
                     WRWYGKAELVRLNTIGLLKTAGLTLAQIRQVTMLSARGPSLRQVLEMQIDAWEQRKAE
                     AVHGHAVTEAALRSLNVNDSLSVDELCNLVRSMEMSDRSPDSSGPPAKPSAITLDAAI
                     LDRYVGHYKVAAYSVLTITREGGRLLARMPFSLAELTPLSETEFVGSITQSHYTFVTD
                     EEGIATGLMGNLDGIEVTWPRIDDAARDEIKAKLAARIESKTPVPGSESALRRLREGI
                     RNGAPNYDEMSPALAQLCRKQLPQLEMTARFLGDTQSIEFQGVGSQGWDVYEVRCENG
                     TVKWRIALTDGVIVGAAAAFADGP"
     gene            complement(2850..3344)
                     /locus_tag="VFL84_11345"
     CDS             complement(2850..3344)
                     /locus_tag="VFL84_11345"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609264.1"
                     /translation="MSRTTNRARAWRQVGLVLGSVIGILALAAWTSRAEAHHSFSVFD
                     MQTNKEVEGEVVEFQWTNPHTFLWISVKNADGSETRWGLEGMSPNFLGRRGWTKRTFE
                     PGDHIKAVIWPLKSGEPGGTLQRATLPDGSEVVNFGGGGGGGGGGGAARAGGAAPSGG
                     GAPQ"
     gene            complement(3341..4087)
                     /locus_tag="VFL84_11350"
     CDS             complement(3341..4087)
                     /locus_tag="VFL84_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609265.1"
                     /translation="MLRHAWIIAALLAAPLAAQKSGPQGDTWESIAKLPDFGGLWEVT
                     FGGGPRGGGEPPQFTPEYAAKLQAFQAAQQRGEIEDGPAANCVPNGMPSIMTQPYPIE
                     ILFTPGKVTILIEAYAQWRQIFTDGRAHPEDPDLTFNGHSIGHWEGDTLVVDTVGFTT
                     DTFLGNQGMRHSDKMHIVERFRLAQPDRLEVQTTITDTQALTKPITRTAAYGRHRDWT
                     LAEYVCQQNNRNFTTDDGKAGIDLRFEEEK"
BASE COUNT          756 a         1433 c         1384 g          754 t
ORIGIN      
        1 ccggcgtgcg cacgccgcaa tgcctagagt cctcgagctc gcgaagcgct tttacgacgc
       61 cggcgtgcgc ctgtcgatcg gcaccgatgg caccggcggc ggcccgagct tcgtgcgcga
      121 gctcaggctg cacgtccagg ccggcattcc gccgtgggac gtgctgcggc tcgcgacgag
      181 cgacgccgcc gagcggctcg gcatgggtga gctcagggga gccctcgcgc cgggattcga
      241 agccgacatc gtgtttctga acagcaaccc gctcgagagc atcgagaacg caggcgacgt
      301 cgacaccgtc gtgcaagacg gtcgcgcgta tcgcgccaaa gagctcatcg agatggcgtc
      361 agcgccggcg aattgactct atttctggct atgtcgacgg gattccgtca ttgcgcgccg
      421 ccgagcccgg cgcgcacaat cgaagcgccc tccttctcgg atgagccgat gactgccacc
      481 gccgccaaac cagaaaccgt ccgcttgtcg cgcgaagagc tgcgccagcg ctggcgccag
      541 ctcgccgaca atcctctggt ggcggcgctc tcgtacaagg ttgagctcaa cgagaagggc
      601 gcgatcgtag tgagcccgcc gactgttcgt catgcgttca ttcaaggctt cttgacgcgc
      661 gagctggcgc gtcaacggcc cgagggtaca acgctcccgg agtgtcccgt cgaaacggag
      721 atcggcatgc gcgtgcccga tgtcgtttgg gcatcgccgg atttcatgaa gcagcaccgc
      781 aacgagaagg agttgagtgc cgcaccggag ctctgtatcg aggtgctgtc gccgacgaat
      841 acgcgggccg agatcgcaga aaagatcgcg gcctatctct ccgcgggggc aaaggaagtt
      901 tgggtcgttg gtgacgacgg cgcagctgaa atccacgtga gcgccggccg agtggctgca
      961 accacgctca ggttcgacct agcagccctc cggcgagttg attagtcgac ggcgccggcc
     1021 gccaacaaga cctgcaccgt ggcaacatgc ctgcgctcag aagcgaggcg caacgcgctc
     1081 gagccgtcgc cccgcgtgtc attcacgttg gcaccgagcg cgagcagagt cttgacgaca
     1141 tctgtgtggc cattcgccgc cgccatgaag aacgcggttt cgccgaagtt ggtcttgcga
     1201 tcgatggccg cgcctgcggc gaccagcatg tcgacgatcg ccttgtgtcc cttcgccgaa
     1261 gcgagcatca gcggcgtcca tccgggcctt gcttgaaaat cgaaatcggc cttggcttcg
     1321 actagcgccg tcgcgatttc gagatgcccg ttggctgcgg cgagatgcag cgccgtttgt
     1381 ccgtccgcgc gtggcgtgtt gacggccgct ccgcgcgtca acagttcctg cacgacggcg
     1441 agatatcccc gctgcgctgc cacgactaga gcagtctccc cgcccgctgt cttcgtgtag
     1501 ttcaagtcga cgccgctctc tgccagcgct cgaaccgcgg cgacgtcgcc gcgcgtcgcg
     1561 gccgccagca acgcttcatt cgcgccggca accggtgtta cgccaaacaa ggcgaccgca
     1621 gcggaccaac ccaacgcccg cgacacggtt cgccgtcctt tttgcatcag catcccctgg
     1681 agactcgaca attcgatcga aggcttgcac cggacctaac gtcatagtct agagtgcagc
     1741 cgcacgtggc agaccacgac gacaaagagg acggcctcac cgccgccgaa tgcgcggccc
     1801 gcacgggcct gaccgttcgc gcgctgcgag tgtacgagga ctacgggctg cttgcaccga
     1861 agcgcaccgc cggcggctgg cgttggtacg gcaaagccga gctcgtcagg ctcaatacga
     1921 tcggactgtt gaagactgcg ggactcacct tggcgcagat ccgtcaggtc acgatgctaa
     1981 gcgcgcgggg cccgtcactg cggcaagtgc tcgagatgca gatcgatgcc tgggaacaac
     2041 ggaaagccga ggctgtgcac ggacatgccg tgaccgaagc cgcgttgcgc agtctcaatg
     2101 tgaatgactc gttgtcggtc gacgagctct gcaatttggt gaggagcatg gaaatgagcg
     2161 acagatcccc cgactcttcg ggcccgccgg caaagccgtc cgccatcacg ctcgatgccg
     2221 cgatcctcga tcgctacgtc ggccactaca aggtggccgc ctactcggtc ctcacgatta
     2281 ctcgcgaggg cggtcgactg ctcgcgcgaa tgccgttctc gctcgcggag ctcacaccgc
     2341 tcagcgagac ggagttcgtg ggctctatta cgcaatcgca ctacacgttc gtgacggacg
     2401 aagaaggcat cgcgacgggc ctcatgggca atctagacgg catcgaggtc acttggcctc
     2461 gcattgacga cgctgcgcgc gacgagatca aagcgaaact ggcggcgaga atcgagagca
     2521 agacgccggt ccctggcagc gaatcggcac tgcgtcgcct gcgcgagggc atacgcaacg
     2581 gcgcaccgaa ctacgacgag atgagccccg cactggcgca actctgccgc aagcaacttc
     2641 cgcaacttga gatgaccgcc agattcctag gcgacactca gtcgatcgag ttccaaggcg
     2701 tcggtagcca aggatgggac gtgtacgagg tccgctgcga gaacgggacc gtgaaatggc
     2761 gcatcgcgtt gacggatgga gtcatcgtcg gcgcggcggc ggcctttgcg gacgggccgt
     2821 aagggaagaa ggagcgctcg agcgattctc tactgtggcg cgccgccgcc agaaggcgcg
     2881 gcaccgccgg cgcgcgcagc tccgcccccg ccgcccccgc caccgccacc gccgaagttg
     2941 acgacctcgc tgccgtccgg caatgtcgcg cgctgcagcg tgccgccggg ctcgccgctc
     3001 ttgagcggcc aaatcacggc cttgatgtga tcgccgggct cgaaggtccg tttcgtccag
     3061 ccgcgccggc ccaagaagtt cgggctcatg ccctcgaggc cccagcgcgt ctcgctgccg
     3121 tcggcattct tcacgctgat ccacaaaaaa gtgtgcggat tcgtccactg gaactcgacg
     3181 acctcgcctt cgacctcctt gttcgtctgc atgtcgaaca cgctgaacga gtgatgcgcc
     3241 tcggcacggg acgtccatgc cgcgagcgcg agaatgccga tgacgctgcc gagcacgagg
     3301 cccacttgcc gccaagcgcg agcgcgattc gtcgttctgc tcatttctct tcctcgaacc
     3361 tgagatcgat tccagccttg ccgtcgtcgg tcgtgaagtt gcggttgttc tgctggcaca
     3421 cgtactcggc gagcgtccag tcgcgatggc ggccgtatgc ggccgtccgt gtgatcggtt
     3481 tggtcagcgc ttgcgtgtcc gtgatcgtcg tttgcacttc gagccgatcc ggctgcgcca
     3541 agcgaaagcg ctcgacgatg tgcatcttgt cgctgtggcg catgccctgg ttgccgagga
     3601 acgtatcggt cgtgaagccg acggtgtcga cgacgagcgt gtcgccttcc caatggccga
     3661 tggagtggcc gttgaacgtg agatccggat cctcgggatg cgcgcggccg tccgtgaaga
     3721 tctggcgcca ctgcgcgtac gcctcgatca ggatcgtcac cttgcccggc gtgaacagaa
     3781 tctcgatcgg atacggctgc gtcatgatcg acggcatgcc gttcggcacg cagttcgcgg
     3841 cggggccgtc ctcgatctcg ccgcgctgct gcgccgcttg gaacgcttgc agcttcgcgg
     3901 cgtactcggg cgtgaattgc ggaggctctc cgccgccgcg cggaccgccg ccgaacgtga
     3961 cttcccacaa gccgccgaaa tccggcagct tggcgatgga ttcccaggtg tcgccttgcg
     4021 ggcccgactt ctgcgccgca agcggcgctg cgaggagcgc cgcgatgatc caagcgtggc
     4081 gtagcatgct tcccccttct gcctgccgca tacactagca gcccgcgggg cgccggcagt
     4141 cttgagaata ctcggtggtg cacgccgccg ccgcacgggc ctcgcgctgg cacgcgactt
     4201 gctgctcgcg accatgactg ccgtgtgttt ccgcttcgtt ttgctcggca cagtgctcgc
     4261 cgtaacggcc ggctgccacc cgccaaacgt tccaaagcga gtcgaagccc gtggcgacca
     4321 agctcaa
//
LOCUS       DASSNN010000611         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_844733, whole genome shotgun sequence.
ACCESSION   DASSNN010000611 DASSNN010000000
VERSION     DASSNN010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844733"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFL84_11355"
     CDS             <1..753
                     /locus_tag="VFL84_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7609266.1"
                     /translation="PKPDYALGFHVWAQIAAGTVSVPQRIAMSSADSVDIAVHGVGTH
                     GASPHRGVDPVLVASQIVVSLQSLVSRSIDPLEDGVVTVGAIHGGTKHNIIGDRVDLQ
                     LTVRADNYETRAKLLEGIKRVADGVARSLGVPEDKLPDVVVSPTESTPPTLNDEETAA
                     RIQSALRAALGNERVPDYKRDGMGAEDFAYYGAPERGVKAVFFMVGGTPQSELANVAA
                     HHSPLFKIAPEPSITTGIEAMVVGAMTLLGHR"
     gene            848..>2279
                     /locus_tag="VFL84_11360"
     CDS             848..>2279
                     /locus_tag="VFL84_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609267.1"
                     /translation="MRMLRALTALTALVAIAAANAQPIPRTPEGKPDLQGIWQVRNRA
                     AYGLEHHDSKYGMPAGPSVVDGGEIPYLPAAREQQRRNFENRAALDPLNNCYMPGVPR
                     IMYMQHPLQIFQTRDHVAITFEWSQVFRLIYANGQPTLHEGIESWMGNSRGRWEGDVL
                     VVEVTDLNDRTWLDAAGNFHSNALRVTERYAMRDANTIEYSATIEDPNVFSRPWTIRT
                     PLHRETDLPRLYEYHCQAEKEEANGDFERDPLTWYPAPIPAGNAPFDARAGAALPAPA
                     RTGEIRRLPDGKPDISGWFELDAGGGNYGLESSPQIFLTPASRGLVVDPPDGKLPYQT
                     WARAERIARGQPHRGYDDPTAHCFLAGIPRSHYVPQPVHILQPPGYVVVLFERMSWRH
                     IAMNARPPLPDHVRLWQGDSHGRWEGDTLVVESKSFNGKAWLNEVGDVVSHMETVVER
                     FTPVDDDTIVYRATVTDPIPYTRPWTI"
BASE COUNT          391 a          794 c          759 g          335 t
ORIGIN      
        1 ccgaagcccg actatgcgct tgggttccat gtctgggcgc agattgcggc cggcacggtc
       61 agcgtgccgc agcgcatcgc catgtcgagc gccgacagcg tggatatcgc cgtgcacggc
      121 gtcggcacgc acggcgcgtc gccgcatcgc ggcgtcgatc ccgtgctcgt cgcgtcgcag
      181 atcgtcgtgt cgttgcagtc gctcgtgagc cgctcgatcg acccgctcga ggacggcgtc
      241 gtgaccgtcg gcgccatcca cggcggcacg aagcacaaca tcatcggcga tcgcgtcgac
      301 ctgcaattga cggtgcgcgc cgacaactac gagacgcgcg cgaagctgct cgagggaatc
      361 aagcgggtcg cggacggcgt cgcgcgctcg ctcggcgtgc ccgaagacaa gctgccggac
      421 gtcgtcgtgt cgcccaccga gagcacgccg ccgacgttga acgacgagga aacggcggcg
      481 cgcatccagt cggcgctgcg cgcggctttg ggcaacgagc gcgtgccgga ttacaagcgc
      541 gacggcatgg gcgccgagga cttcgcctac tacggcgcgc ccgagcgcgg cgtcaaagcc
      601 gtgttcttca tggtcggcgg aacgccgcag tccgagctcg caaacgtggc cgcgcatcat
      661 tcgccgctgt tcaagatcgc gccagagccg tcgatcacga ccggcatcga ggcgatggtc
      721 gtcggcgcga tgacgctgct cgggcaccgt tgacctcgac cgccgggtcc acgtcgcaag
      781 aaagctttag cggcgcgcag cgggcgtcgt cgctggtcac tatacgagct tctatccggg
      841 ggaaggtttg cgaatgttgc gtgcgctgac ggcactcacg gcactcgtcg cgattgcggc
      901 cgcgaacgcg caaccgattc cgcgcacgcc ggaaggcaag cccgacctgc aaggcatttg
      961 gcaggtgcgc aatcgcgcgg cctacggcct cgagcatcac gattcgaagt acggcatgcc
     1021 cgccgggccg agcgtcgtcg acggcggcga gattccatat ctgccggctg cgcgcgagca
     1081 gcaacggcgc aatttcgaga accgcgcggc actggacccg ctcaacaact gctacatgcc
     1141 cggcgtgccg cgcatcatgt acatgcagca tccgttgcag atcttccaaa cgcgcgacca
     1201 cgtcgcgatc acgttcgagt ggtcgcaggt gttcaggctc atctacgcga acggccagcc
     1261 gacgctgcac gaaggcatcg agagctggat gggcaactcg cgcggccgct gggagggcga
     1321 cgtgctcgtc gtcgaggtca cggatctcaa tgaccgcacg tggctcgacg cggccggcaa
     1381 cttccacagc aacgcgctgc gcgtgacgga gcgctacgcg atgcgcgacg cgaacacgat
     1441 cgagtactcg gcgacgatcg aagacccgaa cgtgttctcg cgtccgtgga cgatccgcac
     1501 gccgctgcac cgcgaaacgg atctgccgcg cctctacgag tatcactgcc aggccgagaa
     1561 ggaagaagcg aacggcgact tcgagcgcga cccgctgacg tggtacccgg cgccgattcc
     1621 ggccggcaac gcgccgttcg acgcgcgcgc cggcgcggcc ctgcccgcgc ccgcgcgcac
     1681 gggcgagatc cgccgcttgc ccgacggcaa gccggacatt tctggctggt tcgagctcga
     1741 cgccggcggc ggcaactacg ggctcgagtc gagcccgcag atcttcctga cgccggcgag
     1801 ccgcggcctc gtcgtcgacc cgcccgacgg caagcttccg tatcaaacct gggcacgagc
     1861 ggagcgcatc gcgcgcggac agccgcatcg cggctacgac gatccgaccg cgcactgctt
     1921 cctcgccggc atcccgcggt cgcactacgt gccgcagccg gtgcatattc tccagccgcc
     1981 cggctacgtc gtcgtgctgt tcgagcgcat gtcgtggcgg cacatcgcga tgaatgcccg
     2041 cccgccgctg ccggaccacg tgcgcttgtg gcaaggcgac tcgcacggcc gctgggaagg
     2101 cgacacgctc gtcgtcgaga gcaagagctt caacggcaag gcctggctca acgaggtcgg
     2161 cgacgtcgtg agccacatgg aaaccgtcgt cgagcgcttc acgcccgtcg acgacgatac
     2221 gatcgtctat cgcgccacgg tcaccgaccc gatcccgtac acgcgcccgt ggacgatcg
//
LOCUS       DASSNN010000612         5509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_481326, whole genome shotgun sequence.
ACCESSION   DASSNN010000612 DASSNN010000000
VERSION     DASSNN010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481326"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFL84_11365"
     CDS             complement(<1..451)
                     /locus_tag="VFL84_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7609268.1"
                     /translation="MFEFLFKYSPATYERAELVFASGWPVWLLVALIAAAAVVVGVAI
                     WYRRAGLGLVRGTVLGLLQTALVAGVLVLLWRPALVTQTLRPQENSVAVLLDTSGSML
                     YGNEDASRLQQAVDALSRNALPALESKFAVNLFSFAGELVELPSLDKV"
     gene            679..1455
                     /locus_tag="VFL84_11370"
     CDS             679..1455
                     /locus_tag="VFL84_11370"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET7609269.1"
                     /translation="MTSLGSKLVLAAAVALTAFAAAGDDLRGAPRLPTEELHFARMYY
                     DNYGGGYGGGFRGYGRGSYDTDFPEAEQHFTLGVGRLTRVDIGQDARLIEVTDDMIFD
                     YPWLYAVEVGRWSLDDTEAARLREYLLRGGFLMTDDFHGTQQWQIFVESMQRVFPDRP
                     IVEIPDEDEVKHVVYDLTEHIQIPGIAALRFGVTYEQDGVTPHWRGIYDDEGRLMVAI
                     NFNMDLGDAWELANEPYYPEPMTALATRYAVNYVIYAMTH"
     gene            1566..3275
                     /locus_tag="VFL84_11375"
     CDS             1566..3275
                     /locus_tag="VFL84_11375"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HET7609270.1"
                     /translation="MDGASTTISAAEGLLRALKRRGIDHVLANAGTDFAPVIEGLVRA
                     AGRKDAVPRFITVPHENLAMAMAHGYYQVAGKPAAVMVHVTVGTGNTICALMNAARDN
                     VPLLLMAGRTPLTQDGHIASRSAPIHWGQENFDQAGVVREYTKWDFELRGGQPVDEVV
                     GRALDIALSEPRGPVYLTLPREVLASPHKDSATPLPPTELAMPQPSAQHIDTLAEWIA
                     AAEFPLIVTSNLGRDRAAVRVLAELAERHAIPVAEPHGSCVNLPASHPFKLGHAGTEL
                     LGKADVVLVVDCEVPWYPRYAAPRAGAKVAHLGVDPLFERYPLRAFTAHLAITGNSRV
                     GLELLDEALQARPVQRAAADRRRSGVADFKKKKDAELAAIVERARNEKPIRYAYVGKC
                     VHDALPKNGIVVTELGVAGDHFGLEEPGQLLAVSIGGGLGFGLGASLGAKLAAPDRTV
                     VSTIGDGSYMFGNPTPFHLVSRAAKLPTLTIICNNGRWQAVESATRVVYPDGAAAHAD
                     EMPLVELQPSPEFTKVAEASDAFARRVDDPAELPRAIDAALTAVAGGRQALLDVRMEH
                     GVR"
     gene            3329..3532
                     /locus_tag="VFL84_11380"
     CDS             3329..3532
                     /locus_tag="VFL84_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609271.1"
                     /translation="MRTKIRGGTIETEIYTTEGGYVAIKQPAPSAENEHVCLLRADQL
                     PQLIYELQTLYDDRRSWQSAAVE"
     gene            complement(3752..4387)
                     /gene="maiA"
                     /locus_tag="VFL84_11385"
     CDS             complement(3752..4387)
                     /gene="maiA"
                     /locus_tag="VFL84_11385"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008951465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HET7609272.1"
                     /translation="MRLYSFFRSSAAYRVRIALNVKGIAYETVGVNLPGAAHHAAEFR
                     ALNPQATIPTLDDDGTILWQSLAIIEYLDARYPSPRLIPPEPVARARVQALAQLIACE
                     IHPLNNLRVLKYLRGELKLDEAAVSKWYAHWIAEAFGPLEQLVTRFSGGCYCYGDALS
                     LADVCLVPQLYNARRFSCDVTPYPTLVRIADALSAEPAFAAAAPDRQSDGR"
     gene            complement(4393..5055)
                     /locus_tag="VFL84_11390"
     CDS             complement(4393..5055)
                     /locus_tag="VFL84_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006985068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET7609273.1"
                     /translation="MRAMILAAGRGERMGALTAMQPKPLLAVGDRSLIEHHVVRLAAS
                     GVEEIVINLSYRGSEIRKRLGDGSRFGVAIEYSEEGEPPLETGGGIVHALPLLGRDPF
                     VLVNSDVYTDFDFRVLVERPRVPTLVLVPNPTHNARGDFGLDAAGFVSAAPPLSTYAG
                     VAIFDPGWFAELAPGRRPLKPVLDAAIARRELEGLKFSGLWLDVGTPERLEQARALAA
                     RS"
     gene            5177..>5509
                     /locus_tag="VFL84_11395"
     CDS             5177..>5509
                     /locus_tag="VFL84_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609274.1"
                     /translation="MPAASRRSIIAFWLSLGFAPQASAQDLCLIPEPPPELPQSTVPA
                     TAPSDDDTLEIVTEHIETVSDSEATLGDSQIKYGEGTITAQGLTLAENGDFDLTGRVQ
                     VTAPDVVVF"
BASE COUNT          954 a         1875 c         1865 g          815 t
ORIGIN      
        1 ggaccttatc gagcgagggc agctcgacta gctcgcccgc gaacgagaat agattcacgg
       61 cgaatttgct ctcgagcgcg ggcagcgcgt tgcgcgacag cgcgtcgacg gcttgttgga
      121 ggcgcgaggc gtcctcgttg ccgtacagca tgctgcccga cgtgtcgagc aacacggcga
      181 cggagttctc ctgcggccgc aacgtctgcg tgacgagggc cggccgccac agcagcacga
      241 gcacgccggc gacgagcgcg gtctgcagca ggccgaggac cgtgccgcgc accagaccca
      301 agccggcgcg gcgataccag atcgcgacgc cgacgacgac ggcggccgcc gcgatcagcg
      361 cgacgagcag ccagacgggc caaccgctcg cgaagacgag ctcggcacgt tcataggtgg
      421 cgggagagta tttgaacaag aactcgaaca tcgggctcac catacgctga cgccttcccg
      481 gctgagcccc gcgctgaaca aaactccaga aaccggcagt aaccggcacg agcgccgaga
      541 ggtgtcgggg ttcttaacgg agcttaccgt gcgatctccg ctcgcgacta gaccttccgc
      601 attcactatc gcgggcgaca ggcgccacgc taccatcggt tgaagactcg accgtaccgc
      661 gcaggacgct cgctctcgat gacaagcctc ggctcgaagc tcgtgctcgc cgccgcagtc
      721 gctctgacgg cattcgcggc ggccggcgac gatttgcgcg gcgcgccacg cttgccgacc
      781 gaggagctgc acttcgcgcg catgtactac gacaattacg gcggcggtta cgggggtggc
      841 ttccgcggct acggccgggg cagctacgac acggattttc ccgaggccga gcagcatttc
      901 acgctcgggg tcggccggct cacgcgcgtc gacatcggcc aggacgctcg gttgatcgag
      961 gtcaccgacg acatgatctt cgactacccg tggctgtacg ccgtcgaggt cggccgctgg
     1021 tccttggacg acaccgaggc cgcgaggctg cgcgagtacc tgctgcgcgg cggctttctg
     1081 atgaccgacg acttccacgg cacgcagcag tggcagatct tcgtcgagtc gatgcagcgc
     1141 gtgtttccgg atcgcccgat cgtcgagatc ccggacgagg acgaggtcaa gcatgtcgtg
     1201 tacgacctca ccgagcacat ccagatcccg ggcatcgcgg cgctgcgctt cggcgtgact
     1261 tacgagcagg acggcgtcac gccgcattgg cgcggcatct acgacgacga gggccggctc
     1321 atggtcgcga tcaacttcaa catggatctc ggcgacgcct gggagctcgc caacgagccc
     1381 tactatccag agcccatgac ggcgctcgcg acccgctacg ccgtcaacta cgtcatctac
     1441 gcgatgaccc actaaaaggg gacggatcta tttttctccg aaaatagatc cgtccccatt
     1501 agaaaataga tctgtcccca ttggaggggg ctaaactgcg cgcctagcga acgggagccg
     1561 aagcgatgga cggagcgagc acgacgattt ccgcggccga agggctgctg cgagcgctga
     1621 agcggcgcgg catcgaccac gtgctcgcga acgccggcac cgacttcgcg cccgtcatcg
     1681 aagggctcgt gcgcgccgcc ggacgcaagg acgcggttcc gcgcttcatc accgtcccgc
     1741 acgagaatct cgcgatggcg atggcgcacg gctactacca ggtcgcaggc aagccggccg
     1801 ccgtcatggt ccacgtcacg gtcggcaccg gcaacacgat ctgtgcgctg atgaatgcgg
     1861 cgcgcgacaa cgtgccgctg cttttgatgg cgggccgcac gccgttgacg caggacggcc
     1921 acatcgcgtc gcggtccgcg ccgattcact ggggacagga gaacttcgat caagccggcg
     1981 tcgtgcgcga gtacacgaag tgggacttcg agctgcgcgg cggccagccc gtcgacgagg
     2041 tcgtcgggcg cgcgctcgac atcgcgctgt ccgagccgcg agggcccgtg tatctgacgc
     2101 tgccgcgcga ggtgctcgcg agcccgcaca aggacagcgc gacgccgctg ccgccaaccg
     2161 agctcgcgat gccgcagccg agcgcgcagc acatcgacac gctcgccgaa tggatcgccg
     2221 ccgccgagtt cccgctgatc gtgacgtcga atctcgggcg cgatcgcgcc gccgtacgcg
     2281 tgctggccga gctcgccgag cggcatgcca ttccggtcgc ggagccgcac ggcagctgcg
     2341 tgaacttgcc cgcgagccat ccgttcaagc tcgggcacgc cggcaccgag ctgctcggca
     2401 aagccgacgt ggtgctcgtc gtcgattgcg aggtgccgtg gtacccgcgc tatgcggcgc
     2461 cgcgagccgg cgccaaagtc gcgcacctcg gcgtcgaccc gctgttcgaa cgttatccgt
     2521 tgcgcgcatt cacggcgcat ctcgcgatca ccggcaactc gcgtgtcggc ctcgagctgc
     2581 tcgacgaagc gctgcaagcg cggcccgtgc aacgcgcggc cgcggaccgc cggcgctcgg
     2641 gcgtcgccga cttcaagaag aagaaggatg ccgagctcgc ggccatcgtg gagcgcgcgc
     2701 gcaacgagaa gccgattcgc tacgcctacg tcggcaagtg cgtgcacgac gcgctgccga
     2761 agaacggcat cgtcgtcacc gagctcggcg tcgcgggcga tcacttcggg ctcgaggagc
     2821 cgggacagct gctcgcggtc agcatcggcg gcggcttggg cttcggcctc ggcgcgtcgc
     2881 tcggcgcgaa gctggcggcg ccggaccgca cggtcgtgag cacgatcggc gacggcagct
     2941 acatgttcgg caacccgacg ccgttccacc tcgtctcgcg cgcggcgaag ctgccgacgc
     3001 tgacgatcat ctgcaacaac ggccgctggc aagccgtgga atccgcgacg cgcgtcgtct
     3061 accccgacgg tgcggccgcg cacgccgacg agatgccgct cgtcgagctg caaccgtcgc
     3121 cggagttcac gaaggtcgcc gaagcctccg acgcgtttgc gcgccgcgtc gacgatccgg
     3181 cggagttgcc gcgcgcgatc gacgcggcgc tgacggccgt cgcgggcggc cgccaggcct
     3241 tgctcgacgt gcgcatggag cacggcgtcc gctgacaccc gcggcacgag atatgcattc
     3301 cgcaacgcgg gcgttacagg agctcgtcgt gcggacgaag atcagaggcg gaacgatcga
     3361 gaccgagatc tatacaaccg agggcggcta cgtcgcgatc aagcagccgg ccccgagcgc
     3421 ggagaacgag cacgtctgcc tgctcagggc ggaccagctg ccgcagctca tatacgagct
     3481 acaaacgctc tacgacgacc gccggagctg gcaaagcgct gcagtcgaat agccgccgcg
     3541 cgtgcgggcg gatcgctcat cgtgggcgag ccgccgctgc acggcacgtt ccaggcgctg
     3601 cccaccccaa acgcgagccg tcgatgtgga ggggcgcttc cgagaccgtg tgcgcacagg
     3661 gatgtgcgcg cacgagccgc agggacgctc ttccggcgtg tctcggaagc gcccctccac
     3721 agcggcggct cgcccgcgac gagcgaaccg cctaccgccc gtctgactga cggtcgggtg
     3781 ctgcggctgc aaacgcgggc tccgcggaga gcgcgtcggc gatgcggacg agcgttggat
     3841 aaggcgtgac gtcgcacgag aagcggcgcg cgttgtagag ctgcggcacg aggcagacgt
     3901 cggcgagcga cagcgcatcg ccgtagcagt agcagccgcc gctgaagcgc gtgacgagct
     3961 gctcgagcgg gccgaaggct tcggcgatcc agtgcgcgta ccacttgctg acggccgctt
     4021 cgtcgagctt cagctcgccg cgcaggtatt tcagcacgcg caagttgttc aacggatgga
     4081 tctcgcacgc gatcagctgc gcgagcgctt gaacgcgcgc gcgtgccacc ggctcgggcg
     4141 gtatgagccg cggcgacggg tagcgcgcgt cgaggtattc gatgatcgcg agcgattgcc
     4201 agaggatcgt gccgtcgtcg tcgagcgtcg ggatcgtcgc ttgcggattc agcgcgcgaa
     4261 actccgccgc atggtgcgcc gcgccgggca aattcacgcc gacggtttcg tacgcgatgc
     4321 ctttgacgtt cagcgcgatg cggacgcgat acgcggccga gctgcggaag aacgagtaga
     4381 gacgcatgcg atctagctcc gcgcggccaa ggcgcgcgcc tgctcgagcc gttccggcgt
     4441 gcccacgtcg agccagagcc ccgagaactt gagaccttcg agctcgcggc gcgcgatcgc
     4501 cgcgtcgagc acgggcttca gcggccggcg gcccggagcg agctccgcga accatcccgg
     4561 gtcgaagatc gcgacgccgg cgtaggtcga gagcggcgga gccgcgctga cgaatcccgc
     4621 ggcgtcgagc ccgaaatcgc cgcgcgcatt gtgcgtcggg ttcggcacga gcacgagcgt
     4681 cggcacgcgc ggccgttcca cgagcacgcg aaagtcgaag tccgtgtaca cgtcggagtt
     4741 cacgagcacg aacgggtcgc ggccgagcag cggcagcgcg tgcacgatgc cgccgcccgt
     4801 ctcgagcggc ggctcgccct cttcgctgta ttcgatcgcc acgccgaagc ggctgccgtc
     4861 gccgaggcgt ttgcggatct cgctgccgcg gtacgagaga ttgatgacga tctcctcgac
     4921 gccgctcgcg gcgagccgca cgacgtggtg ctcgatcagc gaacgatcgc cgaccgcgag
     4981 cagcggtttc ggttgcatcg ccgtcagcgc gcccatgcgc tcgccgcggc cggccgcgag
     5041 gatcattgct ctcatgcgat gacgatcaaa cgcgtacggt acgcgatctc aaccaacacg
     5101 ttcaggaatc caggcaagcc gttgaagcga cgcggtgcgt gtgccattgt agctgccttt
     5161 aattcagccg cacctcgtgc ccgccgcaag ccgccgatcc atcatcgcgt tctggctctc
     5221 ccttggcttt gcgccgcaag cctccgcgca agatctctgc ttgattcccg agccgccgcc
     5281 ggagctgccg cagtccaccg tccccgcaac cgcaccgagc gacgacgaca cgctggagat
     5341 cgtcacggag cacatcgaaa ccgtatcgga ttccgaagcg acgctcggcg actcgcagat
     5401 caaatatggc gagggcacga tcacggcgca gggcttgacg ctcgcggaaa acggcgactt
     5461 cgatttgacc ggccgtgtcc aggtgacggc gcccgacgtc gtcgtcttc
//
LOCUS       DASSNN010000613         3568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1617783, whole genome shotgun sequence.
ACCESSION   DASSNN010000613 DASSNN010000000
VERSION     DASSNN010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617783"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..506)
                     /locus_tag="VFL84_11400"
     CDS             complement(63..506)
                     /locus_tag="VFL84_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7609275.1"
                     /translation="MALVRYEPWGIGQLQSEINRLFRSFGDWDSSGATAGWVPPVDIY
                     EHGDRFQLYMDLPGVDPSSVDITLEGGVLTVSGERTQFTGPRDTNDQGELMRSERGLG
                     RFYRRFVLPDSVDSDRVKATNRHGVLELTIPKQAKALPRRIKVAA"
     gene            complement(664..1392)
                     /locus_tag="VFL84_11405"
     CDS             complement(664..1392)
                     /locus_tag="VFL84_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET7609276.1"
                     /translation="MIMRARIALYVAAATVAAQATMAEVSERIMRVAGTAVSYKLVLP
                     NGYDEAMTYPAILVFGGGPQTMNTIDGALARNFQLEAERRGYIVIGPAAPDGDLFFYD
                     GDRIFPEFLDMILAAYKIEAGKFHVAGPSNGGIAAMHVAAKHPEYFLSATAFPGYLWQ
                     PTEAKLRKLEGICAYFYVGEHDEYRWHGEMRREAEYLSSHGSVAQFSEEKGQPHRIET
                     LAGAGASRLFDGFEAARRGCTRRP"
     gene            complement(1389..2063)
                     /locus_tag="VFL84_11410"
     CDS             complement(1389..2063)
                     /locus_tag="VFL84_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609277.1"
                     /translation="MPLPAEQTGPSSWLDAALARFAADNGDLAPRTRAVLDVIVAEPK
                     RHARMLNTLSLLEHMGSHKIMATQQSAAIDQATLKHVAEEAHHAFFMKRQAEKTAERA
                     LEYVADDLLAAPSARMYFQRLEGATVRALGERRSARAAYLYMSMIVEFRALWFYGLYE
                     QALKRARHALTLKRVLGEEQAHLSDMAERLESGNELDGARTAQVLAAERKLFSRLLGA
                     LERAAA"
     gene            complement(2135..2965)
                     /locus_tag="VFL84_11415"
     CDS             complement(2135..2965)
                     /locus_tag="VFL84_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7609278.1"
                     /translation="MKFFPFCLLVVPALVVATACTRKNVPSRVEDRSPDARAAESPEQ
                     AARSTLASQAAHAVPEVDESHLRETAEEYIARRGAETKPFEPGEIAPSAVGPVAACMT
                     CHGAQGEGIPALATPRIGGMPAWYLARELKYFHVGVRAASVDDVQGTQMRAVLLASRT
                     DAAGLEDLAGYFATFDPPPAPALESGNAAHGEQLFATCVACHGVDGRGSAALNTPSLV
                     EQDGDYLVRQIDNFRSGIRGADMRDLFGQQMRAVAGSLITSHEDAVDVVAYIGSLRGD
                     "
     gene            3085..>3568
                     /locus_tag="VFL84_11420"
     CDS             3085..>3568
                     /locus_tag="VFL84_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609279.1"
                     /translation="MLRHAWFIAILVSAPLAAQKSGPQGDTWESIAKLPDFGGLWEVT
                     FGGGPRGAGEPPQFTPEYAAKLQAFQSASQRGEIEDGPAANCVPNGMPSIMTQPYPIE
                     ILFTPGKVTILIEAYAQWRQIFTDGRAHPEDPDLTFNGHSIGHWEGDTLVVDTVGFTT
                     D"
BASE COUNT          556 a         1182 c         1229 g          601 t
ORIGIN      
        1 cccggggggc gacatccccc cggacggtcc gcattgccga tccttcaagc ctctcggctg
       61 agctaggccg caaccttgat gcgtcgcggc aaagccttcg cttgcttcgg gatcgtgagc
      121 tcgagcacgc cgtggcggtt cgtcgccttg acgcggtcgg agtccacgct gtcgggcaac
      181 acgaagcggc gatagaagcg gccgaggccg cgctcgctgc gcatcagctc gccctggtcg
      241 ttcgtgtcac gcggaccggt gaactgcgtg cgctcgccgc tgacggtcaa cacgccgccc
      301 tcgagcgtga tgtccacgct gctcggatcg acgccgggaa ggtccatgta gagctggaac
      361 cgatcgccgt gctcgtagat gtcgacgggc ggaacccagc ccgcggtcgc gccgctcgag
      421 tcccaatcgc cgaacgagcg gaacaggcgg ttgatttcgc tctggagctg cccgataccc
      481 cacggttcgt atctgacaag tgccatagtg agtcatcctc cttgtctatc gtctgaactg
      541 gacgccccgt ttttccgggg cgcccgaaac ggccgttttc ttagggttcg agctggtttt
      601 tttcaaggcc ccgcgtatgg cggccttggg gccgaggagc tccgccccga gcgaattggc
      661 gcctcagggg cgccgggtgc acccgcgccg tgcggcctcg aagccgtcga aaaggcggct
      721 cgcccccgcg ccggcgagcg tctcgatgcg atgcggctgg cctttctctt cgctgaactg
      781 cgcgacgctg ccgtgagagc tcaagtattc ggcttcgcgc cgcatctcgc cgtgccagcg
      841 gtactcgtcg tgctcgccga catagaagta ggcgcagatg ccctcgagct tgcgcagctt
      901 cgcttcggtc ggttgccaga gataaccggg gaacgccgtc gccgagagga aatactcggg
      961 gtgcttggcc gcgacgtgca tcgccgcgat gccgccgttc gagggcccgg cgacgtggaa
     1021 cttgccggcc tcgatcttgt aggcggcgag gatcatgtcg aggaactcgg ggaagatgcg
     1081 atcgccgtcg tagaagaaga gatcgccgtc cggcgccgcg ggcccgatca cgatgtagcc
     1141 gcgccgctcc gcctccaatt ggaagttgcg cgcgagcgca ccatcgatcg tgttcatcgt
     1201 ttgcgggccg ccgccgaaga cgaggatcgc gggataggtc atagcctcgt cgtagccgtt
     1261 cggcagcacg agcttgtagc tgaccgcggt gccggcgacg cgcatgatgc gctcgctgac
     1321 ttcggccatg gtagcttgtg cggccacggt cgcggccgcg acatagagcg cgatgcgcgc
     1381 gcgcatgatc atgcggccgc ccgctcgagc gcgccgagca atcgcgaaaa gagcttccgc
     1441 tccgcagcga gcacttgcgc ggtgcgcgcg ccgtcgagct cgttgccgga ctcgagccgt
     1501 tcggccatgt cgctcaaatg cgcttgctcc tcgccgagca cgcgcttcaa cgtgagcgcg
     1561 tgccgcgccc gcttcagcgc ctgctcgtac aagccgtaga accagagcgc gcgaaactcg
     1621 acgatcatcg acatataaag gtaggcggcg cgcgcgctgc gtctttcgcc cagtgcgcgc
     1681 acggtcgcgc cctcgagccg ctggaagtac atgcgcgcgg aaggggccgc gagcagatcg
     1741 tcggcgacat attcgagcgc gcgctcggcc gtcttctcgg cctggcgctt catgaagaat
     1801 gcgtggtgcg cttcctcggc gacgtgcttc agcgtcgctt ggtcgatcgc ggcggactgc
     1861 tgcgtcgcca tgatcttgtg gctgcccatg tgctcgagca gcgacagcgt gttgagcatg
     1921 cgcgcgtggc gcttcggctc ggcgacgatg acgtcgagca cagcgcgcgt gcgcggcgcg
     1981 agatcgccgt tgtcggcggc gaaccgcgcg agcgcggcat cgagccacga gctcggcccc
     2041 gtctgttctg cgggaagcgg catgctctag agaatctgga cacccctgtc gtcggggttg
     2101 ctagtgtact tgaatccgcg ccgggaacgg cgcgtcagtc gccgcgcaga ctgccgatgt
     2161 aagcgacgac gtcgacggcg tcctcgtggc tcgtgatgag gctcccggcg accgcgcgca
     2221 tctgttggcc gaagaggtcg cgcatgtcgg cgccgcggat gccgctccgg aagttatcga
     2281 tctggcgcac gaggtagtcg ccgtcttgct cgacgaggct cggcgtgttc agcgccgcgc
     2341 tgccccggcc gtcgacgccg tgacacgcga cgcaggtcgc gaagagctgc tccccgtgcg
     2401 ccgcgttgcc cgactcgagc gccggagcgg gcggaggatc gaacgtcgcg aagtagccgg
     2461 cgaggtcttc gagccccgcc gcgtcggtcc gcgatgcgag cagcacggcg cgcatttgcg
     2521 tgccttgcac gtcatcgacg gacgcggcgc gcacgcccac atgaaaatac ttgagctcgc
     2581 gcgcgagata ccaggccggc atgccgccga tgcgcggcgt ggcgagcgcc gggatccctt
     2641 cgccctgtgc gccgtggcag gtcatgcatg cggcgacggg cccgactgcg ctcggcgcta
     2701 tctctccggg ttcgaacggc ttcgtttccg cgccgcgccg ggcaatgtac tcctcggccg
     2761 tctcgcgaag atgcgactcg tcgacctcgg gcacggcgtg agccgcctgc gacgccagcg
     2821 tcgacctcgc cgcttgctcg ggggactcgg cagcccgggc gtccggcgat cgatcctcga
     2881 cgcggctcgg cacattcttg cgcgtgcacg cggtcgcaac gacgagcgcg gggaccacga
     2941 gcagacagaa ggggaaaaat ttcatggcag caagtagcaa gtcgcgtgcc aggatggccg
     3001 cgccggcacg gccgattgaa aattctccag accgccgaag cgcggcgggc tgctagtgta
     3061 tgcgccaggc acagggggga aagcatgctg cgccacgcgt ggtttatcgc aattctcgtc
     3121 agcgcgccgc tcgcggcgca gaagtccggc ccgcagggcg acacctggga atcgatcgcg
     3181 aagctgcccg acttcggcgg cttgtgggaa gtcacgttcg gcggcggtcc gcgcggcgcg
     3241 ggagagcctc cgcaattcac gccggagtac gccgcgaagc tgcaagcgtt tcagtccgcg
     3301 agccagcgcg gcgagatcga ggacggcccc gcggcgaact gcgtgccgaa cggcatgccg
     3361 tcgatcatga cgcagccgta tccgatcgag attctgttca cgccgggcaa ggtgacgatc
     3421 ctgatcgagg cgtacgcgca gtggcggcag atctttacgg acggccgcgc gcatcccgag
     3481 gatcccgacc tcacgttcaa cggccactcg atcggccatt gggaaggcga cacgctcgtc
     3541 gtcgacacgg tcggcttcac gaccgata
//
LOCUS       DASSNN010000614         5190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_481425, whole genome shotgun sequence.
ACCESSION   DASSNN010000614 DASSNN010000000
VERSION     DASSNN010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481425"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /locus_tag="VFL84_11425"
     CDS             <1..1746
                     /locus_tag="VFL84_11425"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HET7609280.1"
                     /translation="RIVESGSRELDNLSRALRGGFVPVGGGGEPIRNPDSYPTGKNFY
                     GIDPEKVPKRASWELGVKLADEMLAKHLEEHGRYPEKVSFVIWGDETMRHEGVLESQI
                     FWLLGTRPVWNDRDKVVDVEVIPSSELKRPRVDIVIASAAEGMFNNVTLLMDKAVQEV
                     KALEEAENFVRRHYLATRATLIAAGYSEEDADRRAGVRIFDEPPGTFNLNTSKIAEAS
                     GTWNSDTGLGDDYLRKMGHGFGNGFWGEPMEDVFRLALSGTEKVVHSSSTMLYGALDN
                     DDFYMYMGGLASAVRNLDGASPELVVTNTRDPGKPEMSSIDEFIGSEFRSRYVNPTWI
                     EGMQKEGYAGAGAMRELVEYLWGWDATVSETVDDAMWQETFDVYVKDKHDLDMKRFFD
                     EKSPFAFQDIAARMLETARKGYWQADDETRATLVREYLESVAKHGVGCSDNTCGNAQL
                     LEYVVEQARAANVPAPAIDAFVAEVERATGAPIETLAAAAREFIAANEAKIASRVVPG
                     PSEAPQTENVDEAELRGYRLQNVNEPKLAEAANSAETRAPTPAESSRTLELLALAAFV
                     LGVLLVWRQRRRRAA"
     gene            1748..2902
                     /locus_tag="VFL84_11430"
     CDS             1748..2902
                     /locus_tag="VFL84_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609281.1"
                     /translation="MPRAGRAVCSCAVLVAAAACSPPRQISESGFGAFEVSLAAWNDG
                     LAVAWYDTRDGNAEVYVRRIDADGRKAGPELRLTTTEAESYEADIAPLTDAFAVAWYE
                     KAGDGAMRAQLGVWRRDGTPVWSAPVAVGTGSSRNPVVRSFADALFCAWIEADAERRE
                     FVWGGWWSRDGQPRGDPVRLGAAGETTWNLNAAIAPNGDAWVVFDADAGTRVDELFAA
                     KVSDGIVTLAQLTADDGIRSKYPDLALEAGRAAITWFDERDGNREVYLAAGAAHELLL
                     PIEQRARRITATPGASIGAYLAWSGDRIGLAWSDDSDGNYEVYFQPFDAGGAALAPAR
                     RLSDTRKSSVIPAIKPWRDGFALAWDEVAPARGGVHGDDMRAEVVFTTVP"
     gene            complement(2913..3521)
                     /locus_tag="VFL84_11435"
     CDS             complement(2913..3521)
                     /locus_tag="VFL84_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HET7609282.1"
                     /translation="MLAFFGVRPGMTALDVNAATGWYSEILARVVGPNGRVIAHNHPG
                     ARATLSAAALEARYGGNRLPNVEQLFARHDELRLPVGSLDVVLMSLVYHDTYWHRDGV
                     DWGPIDREALLASLRAALKPGGIVGVVDHYASAGRDPFESVMAVHRIDRAVVLRDFAA
                     AGFVLDGESDVLRARTDDHSLSVFDAAVVGRTDRFVMRFRKP"
     gene            complement(3581..3958)
                     /locus_tag="VFL84_11440"
     CDS             complement(3581..3958)
                     /locus_tag="VFL84_11440"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609283.1"
                     /translation="MKLASLGLTALLIVGTALAHHSPIVFDRTRQVTLTGVVTEFRWG
                     NPHSWIHIDVADADGKVNDWSIEMDPASHLAREGWKSTTLKPGDKISVVVFPLRNDEK
                     GGQYISVTLPDGKKLGRTADGVI"
     gene            complement(3970..4761)
                     /locus_tag="VFL84_11445"
     CDS             complement(3970..4761)
                     /locus_tag="VFL84_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609284.1"
                     /translation="MTVLSWPCVVAVLGFMAGTAAAQSAEPALPPGALSPENLAKPRP
                     KAPFDLTGTWQHELKGPQSWKFVPPAFELTPEAQVHYDAGRKAAAENKVYRDDIGQCW
                     PAGMPLIMTRVHPFLLMQYPTAIFMVQNFMNSLRIIYTDGRPHSDPDVVVPSWNGESI
                     GHWDGDTLVVDTKYFPGHHHWLDQGGASIPASDQLHIVERIRMIENGETLEIEYTMTD
                     PKTFKGEWKTTKRFNRQHDVDIYEVECTPDLNENLPATSSESLIR"
     gene            4944..>5190
                     /locus_tag="VFL84_11450"
     CDS             4944..>5190
                     /locus_tag="VFL84_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609285.1"
                     /translation="MNRPGLVLVSLPLLALAAAAYAHNAICDCFDNGDDTITCEGGFS
                     DGAKAVGVPLRVLDTAGKVLVDGKMSDQSDFTFPKPKV"
BASE COUNT          860 a         1687 c         1821 g          822 t
ORIGIN      
        1 cgcatcgtag agtccggctc gcgcgagctc gacaacttgt cgcgcgcgct gcgcggcggg
       61 ttcgtgccgg tgggcggcgg cggggagccg atccgcaatc ccgattcgta tccgaccggc
      121 aagaacttct acggcatcga cccggagaaa gtgccgaagc gcgcatcgtg ggagctcggc
      181 gtcaagctcg ccgacgagat gctcgcgaag cacctcgagg agcacggccg ttatcccgag
      241 aaagtttcgt tcgtcatctg gggcgacgag acgatgcgcc acgagggcgt gctcgagtcg
      301 cagatcttct ggctgctcgg cacgcggccg gtctggaacg accgcgacaa ggtcgtcgac
      361 gtcgaggtga tcccgagcag cgagctaaaa aggccgcgcg tcgacatcgt catcgcgagc
      421 gcggccgagg gcatgttcaa caacgtcacg ctgctcatgg acaaggccgt acaggaggtc
      481 aaggcgctcg aggaggccga gaacttcgtg cgccggcact acctcgcgac gcgcgcgacg
      541 ctgatcgcgg ccggctactc cgaggaagac gccgatcggc gcgccggcgt gcgcatcttc
      601 gacgagccgc ccggcacgtt caatctgaac acctcgaaga tcgccgaggc gagcggcacg
      661 tggaattcgg atacggggtt aggcgacgac tacttgcgca agatgggcca cggcttcggc
      721 aatggcttct ggggcgagcc gatggaggac gtgttccggc tcgcgctctc gggcacggag
      781 aaggtcgtgc actcgagctc gacgatgctc tacggcgcgc tcgacaacga cgatttctac
      841 atgtacatgg gcgggctcgc gagcgccgtg cgcaatctcg acggtgcgtc gccggagctc
      901 gtcgtcacga acacgcgcga tcccggcaag cccgagatgt cctcgatcga cgaattcatc
      961 ggcagcgaat ttcgctcgcg ctacgtgaac ccgacctgga tcgagggcat gcagaaggaa
     1021 ggctacgccg gcgccggtgc gatgcgggag ctcgtcgagt acctctgggg ctgggacgcg
     1081 acggtaagcg aaaccgtcga cgacgcgatg tggcaagaga cgttcgacgt ctacgtcaaa
     1141 gacaagcacg acctcgacat gaaacggttc ttcgacgaga agtcgccgtt cgcgttccag
     1201 gacatcgccg cgcgcatgct cgagacggcg cgcaagggct actggcaggc cgacgacgag
     1261 acgcgcgcga cgctcgtgcg cgagtatctc gaaagcgtcg cgaagcacgg cgtcggctgc
     1321 tccgacaata cctgcggcaa cgcgcagctg ctcgagtacg tcgtcgagca ggcgcgcgcg
     1381 gccaacgtgc cggcgccggc gatcgacgcg ttcgtggccg aggtcgagcg cgccacgggc
     1441 gcgccgatcg agacgctggc cgcggccgct cgagaattca tcgccgcgaa cgaggcgaag
     1501 atcgcgagcc gcgtcgtgcc gggaccctcc gaggcaccgc aaaccgagaa cgtcgacgag
     1561 gcggagctgc gcggctatcg gctccagaac gtcaacgagc cgaagctggc cgaggccgcg
     1621 aattcggcgg agacccgcgc gccgacaccg gcggagtcgt cgcgcacgct cgagctcttg
     1681 gcgctcgccg cgttcgtgct cggagtgctg ctcgtgtggc gccagcggcg ccgccgcgcc
     1741 gcctgacatg ccccgtgcgg ggcgcgcagt ctgctcgtgc gccgtgctcg tggcagcggc
     1801 cgcgtgctcg cccccgcgcc agatcagcga gagcggcttc ggcgccttcg aggttagcct
     1861 tgcggcttgg aacgacgggc tcgccgtcgc ctggtacgac acgcgcgacg gcaacgccga
     1921 ggtgtacgtg cgccggatcg acgccgacgg gcgcaaggca gggcccgagc tgcgcctcac
     1981 gacgacggag gccgaatcgt acgaggccga catcgcgccg ttgacggacg cgttcgcggt
     2041 cgcgtggtac gagaaggcgg gcgacggcgc gatgcgggca cagctcggcg tatggcggcg
     2101 cgacggcacg ccggtctgga gcgcgccggt tgccgtcggc acgggctcga gccgcaatcc
     2161 cgtcgtccgc agcttcgccg acgcgctgtt ttgcgcgtgg atcgaggccg acgcggagag
     2221 acgcgaattc gtttggggcg gctggtggag ccgcgacggg cagccgcgcg gcgatcccgt
     2281 gcgcctcggc gcggccggcg agacgacgtg gaatctcaac gcggcgatcg cgccgaacgg
     2341 cgacgcgtgg gtcgtgttcg acgcggacgc cggcacgcgc gtcgacgagc tgttcgccgc
     2401 gaaggttagc gacggaatcg tcacgttggc gcagctgacg gccgacgacg gcattcgctc
     2461 gaagtatccc gatctcgcgc tcgaggcggg ccgtgccgcg atcacgtggt tcgacgagcg
     2521 cgacggcaac cgcgaggtct atctggcggc gggcgctgcg cacgagctgc tgctgccgat
     2581 cgagcagcgt gcgcgccgca tcacggcgac gcccggcgcc tcgatcgggg cctatctcgc
     2641 ctggagcggc gatcgcatcg gcctcgcgtg gtccgacgac agcgacggca attacgaagt
     2701 gtattttcag ccgttcgatg cgggcggcgc ggcgctcgcg ccggctcggc ggctcagcga
     2761 cacgcgcaag agctcggtga tcccggcgat caagccgtgg cgcgacgggt tcgcgctggc
     2821 ttgggacgag gtagcgcctg cgcgaggcgg cgtgcacggc gacgacatgc gcgcggaggt
     2881 cgtcttcacg acggtgccgt gacgctccac gctcacggct tgcggaagcg catcacgaat
     2941 cggtccgtgc ggccgacgac ggccgcgtcg aacacgctca gcgaatgatc gtcggtccgt
     3001 gctcgcagca cgtcgctctc gccgtcgagc acgaagcccg ctgcggcgaa gtctctcaga
     3061 acgaccgcgc gatcgatgcg atgcacggcc atcacagact cgaacgggtc ccggccggcc
     3121 gaggcgtagt ggtcgacgac gccgacgatg ccgcccggct tcaaagcagc gcgcaacgac
     3181 gcgagcagcg cctcgcggtc gatcggcccc cagtcgacgc cgtcgcggtg ccagtacgtg
     3241 tcgtggtaga ccaacgacat cagcacgacg tcgaggctgc ctacgggcaa gcgcagctcg
     3301 tcgtgccgcg cgaagagctg ctcgacgttc ggcaatcgat tgccgccgta gcgcgcctcg
     3361 agggcggcgg cggaaagagt cgcgcgcgcg cccggatggt tgtgcgcgat cacgcggccg
     3421 ttcggcccga cgacgcgcgc gagaatctcc gagtaccagc ccgtcgcggc gttcacgtcg
     3481 agcgccgtca tgccggggcg cacgccgaag aaagcgagca cttcgtcggg cttgctcgtc
     3541 gcgctgcgtg cgcgctctag ctcggccgtc gcgtcgacgc tcagatgacg ccgtcggcgg
     3601 tgcgaccgag cttcttgccg tccggcagcg tgacggagat gtactggccg cctttctcgt
     3661 cgttacgcag cggaaagacg acgacgctga tcttgtcgcc gggcttcaac gtcgtcgact
     3721 tccaaccttc gcgcgcgagg tggctcgccg gatccatctc gatgctccag tcgttgactt
     3781 tgccgtcggc atcggcgacg tcgatgtgaa tccacgagtg cgggttgccc cagcgaaact
     3841 ccgtgacgac gcccgtcagc gtgacctgcc gcgtgcggtc gaagacgatc ggggagtggt
     3901 gcgccagcgc cgtgccgacg atcaacaacg ccgtgagacc caagcttgcg agtttcatga
     3961 tcgtcacctc tagcgaatca gcgattcgga cgacgtcgcg ggcaaattct cgttgaggtc
     4021 cggcgtgcac tcgacctcgt agatgtcgac gtcgtgctgg cgattgaagc gcttcgtcgt
     4081 cttccactcg cccttgaacg tcttcgggtc cgtcatcgtg tactcgatct cgagcgtctc
     4141 gccattctcg atcatgcgga tgcgctcgac gatgtgcagc tggtcgctgg ccggaatgct
     4201 cgcgccgcct tggtcgagcc aatgatggtg gcccgggaag tacttcgtgt cgacgacgag
     4261 cgtgtcgccg tcccagtggc cgatcgactc gccgttccaa ctcggcacga cgacgtccgg
     4321 gtcgctgtgc ggccggccgt cggtgtagat gatgcgcagg ctgttcatga agttctggac
     4381 catgaagatc gcggtcgggt actgcatcag caggaacgga tgcacgcgcg tcatgatcaa
     4441 cggcatgccg gccggccagc actggccgat gtcgtcgcga tagaccttgt tctcggcggc
     4501 cgccttgcgg ccggcgtcgt agtggacctg ggcttccggc gtcagctcga aggccggcgg
     4561 aacgaacttc cacgactgcg ggcccttcag ctcgtgctgc caggtgcccg tgaggtcgaa
     4621 cggcgccttc ggccgcggct tggcgaggtt ctccggcgac agggcgccgg gcggcagagc
     4681 gggctccgcg gattgggcgg cggctgtgcc ggccatgaac ccgagcacgg ccacgacgca
     4741 gggccaggaa agaacggtca taagatcccc cttcagctgg cggttagact acctgtgcga
     4801 cgatcccctg tgaaccgtag gcgcgccgcg gtgtttcatg ccgccgcgtt aaccgttagc
     4861 taaccgttga tgacttacga atcacttaac gtctgcggaa aatagcatcg gcgccggcta
     4921 ccccgcgcta gaggaaactt tggatgaaca ggccaggatt ggttttggtt tctctaccgc
     4981 tgctcgcgct ggcggcggct gcctacgccc ataacgcgat ctgcgactgc ttcgacaacg
     5041 gcgacgacac gatcacctgc gaaggcggct tcagcgacgg cgcgaaggcc gtcggcgtgc
     5101 cgctgcgcgt gctcgatacc gcgggcaagg tcctcgtcga cggcaagatg agcgatcaga
     5161 gcgatttcac gtttcccaag cctaaggtcg
//
LOCUS       DASSNN010000615         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1235568, whole genome shotgun sequence.
ACCESSION   DASSNN010000615 DASSNN010000000
VERSION     DASSNN010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235568"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VFL84_11455"
     CDS             <1..966
                     /locus_tag="VFL84_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609286.1"
                     /translation="RFRSDVLTLDVQPAVAPPSSLEGAAWLPAQKVTLTVQWSDPKPD
                     IAVGTPRTRTITIEAVGLLETQLPDIPLEGQAGVRQYADQPELDREVTAGGFKSRRSV
                     SYAVIAQTPGDVTLAGVRLPWWNVTTQRWEVAELPPRTLHVAPTADAAEPLPAPDAQT
                     AVAPGPAPSPTRSSYWPWITGLLAAGWVATLALWWRARPRTRDSRTPPRAPSKPEAKP
                     ALRKILRDLRAACAVSDPAAARNALLDYADVRFAPAPPRSLGALAALLPDSPAREVLA
                     LEAHIYGAAAGAWRGDGLTAVLGELESAAGPPEQAAAEPLLPLYR"
     gene            1054..2274
                     /locus_tag="VFL84_11460"
     CDS             1054..2274
                     /locus_tag="VFL84_11460"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7609287.1"
                     /translation="MDLQRLDPDSYGSPYAEQLKRGFSGLRFNGLLEKDFREFYIGQN
                     LSRARLSGLIGLIIVLAVTCIDLLLGSSGGGTLNTLRLGLLCPLLVMLGVAISVPGAQ
                     RWYTEVVAVGVTLIGFVVIYMAHLGALSGSSYVLAGLVLVVLYGCLFLGLLFNVAVTI
                     AGILVAGHFVTGIVVGLPFDALYYSTAILGGAAVIGGISAYNLEHALRTNFLETRVLN
                     ELAERDGMTGLYNRRIFDDYVERLWRQSRREGNGVAIIFVDIDFFKVYNDLYGHQAGD
                     DCLKKIAKCIARGAKRPFDFAARYGGEEFVLVLYGPPDDYARSVPEQIRRDVLELAIP
                     HAGSQAGKHVTVSVGLAMARPETSRSLAGAIQTADEALYQAKREGRNRVCFKDSDSDD
                     VETGNFRVIQRDLA"
     gene            complement(2277..2696)
                     /locus_tag="VFL84_11465"
     CDS             complement(2277..2696)
                     /locus_tag="VFL84_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7609288.1"
                     /translation="MRYLHTMVRVSNLEQSLDFYCNKLGLKEVRRVDHEAGRFTLVFV
                     AAPGQEDCTIELTHNWDPEPYTGGRNFGHLAYEVDDIYAVCERLRGAGVTINRPPRDG
                     KMAFIRSPDSISIELLQKGAALPKAEPWASMPNTGVW"
     gene            2783..>3220
                     /locus_tag="VFL84_11470"
     CDS             2783..>3220
                     /locus_tag="VFL84_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609289.1"
                     /translation="MTRARIIFVPGLNPKPPPEIYRPQLMRVLHASLRRMRPRAAEWL
                     AENEAQFELAAWTYLFHGAHRDISVDLPGIERLLVQTEAPAEDVLGLGSWTRRAVRWA
                     HLAGDRWPYLGRTFASRPTRRLMHEATRYLKNRDGVGAEVRASV"
BASE COUNT          485 a         1096 c         1071 g          568 t
ORIGIN      
        1 cgttttcgct ccgacgttct cacgctcgac gtgcagccgg ccgtcgcgcc gccttcctcg
       61 ctcgagggag ccgcatggct gcccgcgcaa aaagtcacgc tgacggtgca atggagcgat
      121 cccaagcccg acatcgccgt cggcactccg aggacgcgca cgatcacgat cgaagccgtc
      181 ggcttgctcg agacgcagct gcccgacatt ccgctcgagg gtcaggccgg cgttcgccag
      241 tacgccgacc agcccgagct cgaccgcgag gtcacggcgg gcggtttcaa gtcgcgtcgt
      301 agcgtcagct acgccgtcat cgcgcagacg cccggcgacg tcacgctcgc cggcgtacgg
      361 ctgccgtggt ggaacgtgac gacgcagcgc tgggaagtcg ccgagctgcc gccgcgaacg
      421 ctgcacgtcg cgccgaccgc cgatgccgcc gagccgctgc ccgcacccga cgcgcagacc
      481 gcggtggccc ccggtccggc gccttcgcct acgcgctcgt cgtactggcc gtggatcacg
      541 gggctcttgg ctgccggttg ggtggcgacg ctcgcactct ggtggcgcgc gcgcccgcgc
      601 acgcgcgatt cgcgaacgcc gccgcgagcg ccgtcgaagc ccgaggcgaa gcccgcgctg
      661 cgaaaaattc tgcgcgactt gcgcgccgcg tgtgcggtca gcgatccggc cgcggcacgc
      721 aacgctttgc tcgattacgc cgatgtccgc ttcgcgccgg cgccgccgcg cagcctcggc
      781 gcgttggcgg cgctgctgcc ggactcgccg gcgcgcgagg tgctcgcact cgaggcgcac
      841 atctacgggg cggcagccgg cgcttggcgc ggggacgggc ttacggccgt gctcggcgag
      901 ctcgagagcg cggcaggccc gcccgagcag gccgcggccg agccgctgct gccgctctac
      961 cgataagaac caatcgcgtc tgtgacctag attggagatt cggagccgcg tttcgcgcat
     1021 gcttccggat ctggcacgtc tgcctgcttt ggtatggacc tacagaggct cgatccggac
     1081 agctacggct cgccgtatgc ggagcagctc aagcgcggtt tctccggcct gcgcttcaat
     1141 ggcctgctcg agaaggactt tcgagagttc tatatcggcc agaatctgtc gcgcgcgcga
     1201 ttgtcggggc tcatcgggct gatcatcgtt ctggccgtca cgtgcatcga cctcctgctc
     1261 ggctcgagcg gcggcggcac gttgaacacg ctgcgcttgg ggctgctctg cccgctgctc
     1321 gtcatgctcg gcgtcgcgat ctccgtgccg ggcgcgcagc gttggtacac cgaggtcgtc
     1381 gccgtcggcg tgacgctgat cggcttcgtc gtgatttaca tggcgcatct cggcgcgctc
     1441 tcgggctctt cgtacgtgct cgccgggctc gtcctcgtcg tgctgtatgg ctgcttgttc
     1501 ctcggcttgc tgttcaacgt cgccgtcacg atcgccggca tcctcgtcgc gggacacttc
     1561 gtcacgggca tcgtcgtcgg actgccgttc gatgcgctgt actacagcac ggcgattctc
     1621 ggcggcgcgg ccgtcatcgg cggcatttcg gcctacaacc tcgagcacgc gctgcgcacg
     1681 aacttcctcg agacgcgggt gctgaacgag ctcgcggagc gcgacggaat gacggggctc
     1741 tacaatcgcc gcattttcga cgactacgtc gagcggctgt ggcgccaatc gcgtcgcgag
     1801 ggcaacggcg tcgcgatcat cttcgtcgac atcgacttct tcaaggtcta caacgatctc
     1861 tacggtcatc aggccggcga cgattgcttg aagaagatcg cgaagtgcat tgcacgcggg
     1921 gcgaaacggc cgttcgattt tgcggcgcgc tacggcggcg aggagttcgt gctcgtcttg
     1981 tacggcccgc ccgacgacta tgcgcgcagc gttcccgagc aaattcggcg cgacgttctg
     2041 gagctcgcga taccgcacgc ggggtcgcaa gcgggcaagc acgtgaccgt cagcgtcggt
     2101 ttggcgatgg cgaggcccga gacgagccgc agcctagcgg gcgcgattca aacggccgac
     2161 gaggcgctgt accaggcgaa gcgcgagggc cgcaatcgcg tctgcttcaa ggattccgac
     2221 agcgacgacg tcgagaccgg caacttccgc gtgatccagc gcgatctcgc ctagattcac
     2281 caaacgcccg tattcggcat cgaggcccag ggctcggcct tcggcaacgc cgcgcctttc
     2341 tgcagcagct cgatcgagat agagtcgggc gagcggatga acgccatttt gccgtcgcgc
     2401 ggcggccgat tgatcgtgac gcccgcgccg cgcaaccttt cgcataccgc gtagatgtcg
     2461 tcgacctcgt aggccaagtg gccgaaattt cgaccccccg tgtacggttc gggatcccag
     2521 ttgtgagtga gctcgatcgt gcaatcctct tggccggggg ccgctacgaa cacgagcgtg
     2581 aaccggccgg cctcgtggtc cacgcgccga acctccttga ggccgagctt gttgcaatag
     2641 aagtcgagcg actgttcgag gttgctgacg cgcaccattg tatgcagata ccgcacgacc
     2701 tattctcctt gcgagtcgtc atctcacgag agctgacgac tatacccaag gccatgccgg
     2761 cggtcatagg cgccgcaccg agttgacgcg cgcacgcatc attttcgttc cagggctcaa
     2821 cccgaaaccg ccgccggaga tctatcggcc gcagctgatg cgcgtgttgc acgcgtcgct
     2881 gcgccgcatg cggccgcggg ctgcggagtg gctcgccgag aacgaggcgc agttcgagct
     2941 cgccgcatgg acgtatctgt tccacggcgc gcatcgcgac atcagcgtcg acttgcccgg
     3001 catcgagcgg ctcttggtgc agaccgaagc gccggccgaa gacgtgctcg ggctcggctc
     3061 ctggacgcgg cgcgccgtgc gctgggccca tcttgccggc gaccggtggc cctatctcgg
     3121 ccgcacgttc gcgtcccgtc cgacgcgccg cctcatgcac gaggcgacgc gatatctgaa
     3181 gaatcgcgat ggcgtcggcg ccgaagtgcg cgcgtcggtg
//
LOCUS       DASSNN010000616         5641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_234501, whole genome shotgun sequence.
ACCESSION   DASSNN010000616 DASSNN010000000
VERSION     DASSNN010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234501"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VFL84_11475"
     CDS             complement(<1..1176)
                     /locus_tag="VFL84_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7609290.1"
                     /translation="MPGLLSKERIVAPPSFNRWLVPPASVAIHLCIGSVYAWSIYNPA
                     LIRVFGVVTPAAEDWLLADVVWIFTVAIVFLGLSAAVAGKWLEDVGPRMVGTVSAACW
                     GGGYLLGGLGIYLHELWLVYLGYGVIGGCGLGLGYVSPVSTLIRWFPDRRGMATGMAI
                     MGFGGGAIIGAPLKRFFIRTFYEAPEYLGSVADVSLVTEGGRRFAQTAGGLREVVVVG
                     ANDVGSMIVRGAEGVYAVGTGNVGVAETFITLGLIYFVVMLAAAFSYRLPAPGWKPAG
                     WAPPDEAHRAKRMISTHNVHIDEALKTRQFYQLWIVLCFNVTAGIGMLSVAQTMMTEI
                     FGTTLPTIVDGAFAATYVMMISVFNMLGRFLWASWSDYIGRRNTYWVFFALGIVLYLS
                     "
     gene            complement(1231..1306)
                     /locus_tag="VFL84_11480"
     tRNA            complement(1231..1306)
                     /locus_tag="VFL84_11480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1271..1273),aa:Arg,seq:cct)
     gene            complement(1376..2707)
                     /locus_tag="VFL84_11485"
     CDS             complement(1376..2707)
                     /locus_tag="VFL84_11485"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HET7609291.1"
                     /translation="MNLRLVASCGFVCAALLLSGCDSGRQAPGKVTVQVLNAAPRFAS
                     LQFQREHSEREFGEELFFRDARSYLYDADTYDFYVTERTLRESDPGRIWTFAPTLEER
                     TNYTFVLAEAAGEVQPFVIATPEPTADDARIVLFNAADGLPAMDLYLERPGVGIAGAT
                     PRGTLNALEQVAPPALPSGDYELFLTAAGNPADVLLTSPQFNLPAGTTSTFVVVPDSG
                     QTTALVSVMLIQSTPIVLFDRNAPAQLRGINAATDQAPRDLAVDSQFSPPLLPAMPFA
                     EPTAHAPITVGAVKVNVTPVGNPGVLEIDTQFNAIIGQRVTALFTGPAGALLPIFAAD
                     DLRRIHREAKLRLMNAAARFPGIDFVITLPDGDPNQAQPVAQLLAPGVSDYVPLYPGE
                     YDLYLFQAGTTTVVSGPTRIRVADGGIYAALALDGPDTSTASVRLLDDFVP"
     gene            2929..3882
                     /locus_tag="VFL84_11490"
     CDS             2929..3882
                     /locus_tag="VFL84_11490"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HET7609292.1"
                     /translation="MIAGASVALSAAAASAQLAEPVHVRNLNPLVSIFGLPAWDTVPI
                     GTRFGATAEIANHYRFSLAGNERLMLDGETVRTNFSFTHGFGSGWSLGVELPYYRVSG
                     GVLDDAIDGWHAAFGMPDGGRDARPEGQLLFAFGQPLAPSFVLTEPQTGIGDTQVKFA
                     RLIGADQGFVVQATVKVPTGDEQMLAGSGSADGALTLLRTRPLLARKRAAGYYWGVGL
                     VWAGDPEVVEFDANSWVPTGIVGGSWQVLPAWGLKGQLDVHGAFYDSELEEIGQTAIE
                     ATFSAWRRMGRRGQLDLAVVEDLNVSTAPDVVLQVAAAWQW"
     gene            3870..4373
                     /locus_tag="VFL84_11495"
     CDS             3870..4373
                     /locus_tag="VFL84_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7609293.1"
                     /translation="MAVVSPLLRLGAALCLMLAACDSGRPGAAARTEPPPATAVARAA
                     GFDAGPVKTAAEYLLEPELASADVARGEVLGFACAACHRFRAEEGTLIGPNLHGVFGR
                     RAASVEGFAYSAALRDSGLVWTPRSLEAWLANPADFVEGTTMAFTGYRSAEDRRDLIA
                     YLLHATQ"
     gene            4500..4988
                     /locus_tag="VFL84_11500"
     CDS             4500..4988
                     /locus_tag="VFL84_11500"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HET7609294.1"
                     /translation="MTEPVDREVSAVLPAGVYVERRRGGDRRRLTVRSFLQGGFTPRR
                     RGGRRAGEQHLPIDWHEPYLLFLSLTILLLSFADAFITLTLIMGGAREANPLLAYILR
                     DHPEWFATVKMGLTGAGVLMLVAMARWRLFRMRVGLMLQGIFVAYVALIAYEWWLVRT
                     LL"
     gene            5087..>5641
                     /gene="sucA"
                     /locus_tag="VFL84_11505"
     CDS             5087..>5641
                     /gene="sucA"
                     /locus_tag="VFL84_11505"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF027404.4"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET7609295.1"
                     /translation="MSTVFKHQYDTSHLQGANATYVEWLYEQYLAEPGSVPPEWRQYF
                     STLAAPSAEVPHGPIVQAVAERTRRSAAAPAVAPAAGEGAPAMEKQAAVSRLIQVYSL
                     RGHQIADLDPLGLWERPAPAVLSFDFLGLDESDLQREFSTGGLAGTGHTRMKLQDILA
                     LLKHIYCGKLAAVFAHISRARERLW"
BASE COUNT          920 a         1853 c         1954 g          914 t
ORIGIN      
        1 cgacagatag agcacgatgc cgagcgcgaa gaagacccaa taggtgttgc ggcggccgat
       61 gtagtcggac cagctcgccc agaggaagcg gccgagcatg ttgaacacgc tgatcatcat
      121 gacgtaggtc gcggcgaacg ccccgtcgac gatcgtgggc agagtcgtgc cgaagatttc
      181 cgtcatcatc gtctgcgcga cgctgagcat gccgatgccg gccgtcacgt tgaagcacag
      241 cacgatccag agctgataga actgccgcgt cttcagcgct tcgtcgatgt ggacgttgtg
      301 cgtgctgatc atgcgtttgg cgcgatgcgc ctcgtcgggc ggcgcccaac ccgcgggctt
      361 ccagcccgga gccggcagcc ggtacgaaaa cgcagcggcg agcatcacga cgaaatagat
      421 cagcccgagc gtgatgaacg tctccgcgac gccgacgttg cctgtgccga cggcatagac
      481 gccttcggcg ccgcgcacga tcatgctgcc gacgtcgttg gcgccgacga cgacgacctc
      541 gcgcagcccg ccggccgttt gcgcgaagcg ccggccgcct tccgtgacga gcgacacgtc
      601 ggcgacggag cccaagtact cgggcgcctc gtagaacgtc cggatgaaga atcgcttcag
      661 cggcgcgccg atgatggcac cgccgccgaa gcccatgatc gccatgccgg tcgccatgcc
      721 cctgcggtcc gggaaccagc ggatcagcgt gctgaccggc gacacgtagc ccaaccccaa
      781 gccgcacccg ccgatgacgc cgtagccgag atagacgagc cagagctcgt gcagatagat
      841 gccgaggccg ccgagcagat agccgccgcc ccagcaggcc gcggagacgg tgccgaccat
      901 gcgcggcccg acgtcctcga gccatttgcc ggcgaccgcc gcggaaaggc cgagaaacac
      961 gatcgcgacc gtgaagatcc acacgacgtc ggccagcagc caatcctcgg ccgccggcgt
     1021 cacgacgccg aagacgcgta tcagcgcggg gttatagatg ctccacgcgt agacggagcc
     1081 gatacaaaga tgaatggcga ccgacgcggg cggcacgagc catcgattga acgacggcgg
     1141 cgcgacgatg cgctccttcg agagcaagcc aggcatagtc ccccttcgcg gctcattctg
     1201 gtgtgagcct gagctcaggc cttcaatcgg tggcgccccg ggccgggatc gaaccggcga
     1261 ccttccgctt aggaggcgaa cgctctatcc actgagctac cggggcgcgt gcaactggta
     1321 agtggcgggg aaaagaatac caggtcgcag gcgcacggcg ccgtggccgc tcgcctcagg
     1381 gaacgaagtc gtcgagcagg cgcacgctcg ccgtcgaggt gtccgggccg tcgagggcga
     1441 gggcggcgta gatgccgccg tcggcgacgc ggatcctcgt cggtcccgac actaccgtcg
     1501 tcgtgccggc ttgaaaaaga tagaggtcgt actcgccggg gtagagcggc acgtagtccg
     1561 atacgccggg cgcgagcagc tgagccacgg gctgcgcctg gttggggtcg ccgtccggta
     1621 acgtgatgac gaaatcgatg cccgggaatc gcgccgcggc gttcatgagc cgcagcttcg
     1681 cctcgcgatg gatgcgccgc aggtcgtcgg cggcgaagat cggcagcagc gcaccggcgg
     1741 ggcccgtgaa cagcgccgta acgcgctgcc cgatgatggc gttgaactgc gtgtcgatct
     1801 cgagcacgcc ggggttaccg acgggcgtca cgttgacctt cacggcgccg actgttatcg
     1861 gcgcgtgggc cgtcggctcc gcgaacggca tcgccggcaa caacggcggc gagaactgac
     1921 tgtcgaccgc caggtcgcgc ggcgcttgat ccgtcgcggc gttgatgccg cgcagctgcg
     1981 ccggagcgtt cctatcgaat agcacgatcg gcgtcgactg aatcagcatc acgctgacga
     2041 gggccgtcgt ttggccgctg tcgggaacga cgacgaatgt cgacgtcgtg ccggcgggca
     2101 gattgaactg cgggctcgtc agcaggacgt cggcgggatt gccggctgcc gtcaggaaca
     2161 gctcgtagtc gccgctcggc agcgcgggcg gagcgacttg ctcgagcgcg ttcaaggtgc
     2221 cgcgcggcgt tgcgcccgcg atcccgacgc cggggcgctc gagataaaga tccattgcgg
     2281 gcagcccgtc agccgcgttg aacagcacga tccgcgcgtc gtcagcggtg ggctccggag
     2341 tcgcgatgac gaacggctgc acttcgccgg cggcttccgc cagcacgaac gtgtagttcg
     2401 ttcgctcttc gagcgtgggc gcgaacgtcc agatgcggcc ggggtcgctt tcgcgcagcg
     2461 ttcgctcggt aacgtagaag tcgtaggtat cggcgtcgta gaggtacgag cgcgcgtccc
     2521 tgaagaacag ctcttcgccg aactcgcgct cgctatgctc gcgctggaac tgcagcgacg
     2581 cgaaccgcgg ggccgcattc aagacctgaa cggtgacctt gcccggcgct tgccgcccgc
     2641 tgtcgcagcc cgagaggaga agcgcggcgc agacgaagcc gcacgaagca acgaggcgta
     2701 gattcataaa gggccggtcg cgagctgccg cggttgaagt ggggccgtat ggtacaggct
     2761 agccgcccca ggcgcgaaac ccgcggctag agccgtaatc tgcgcatgac tacaatgcga
     2821 gcgcccgccg ttgctcacat ttgcacgtaa tctgccgcca tgcggcgttc tgactgccgc
     2881 ggcggcggaa gattccacga aaagggctcc tcgatgcgga tcgtcggaat gatagcgggg
     2941 gcgagcgtcg ccctctcggc agcggcggct tcggcgcagc tcgccgagcc cgtgcacgtg
     3001 cgcaacctga acccgctcgt atcgattttc gggctgcccg cctgggacac ggtgccgatc
     3061 ggcacgcggt tcggagcgac cgccgagatc gccaatcact atcgcttctc tctcgccggg
     3121 aacgagcgct tgatgctcga cggcgagacc gtgcggacga acttctcctt cacgcacggg
     3181 ttcgggagcg gctggtcgct cggcgtcgag ctgccgtact accgcgtcag cggcggcgtg
     3241 ctcgacgacg cgatcgacgg ctggcacgcc gccttcggca tgccggacgg cggccgcgac
     3301 gcgcgtcccg aaggtcagct cctgttcgcg ttcggccaac cgctggcgcc gtcgttcgtg
     3361 ttgacggagc cgcaaaccgg tatcggcgat acgcaagtca agttcgcgcg gctcatcggc
     3421 gccgaccagg gcttcgtcgt gcaagcgacc gtcaaggtgc cgacgggcga cgagcagatg
     3481 ctggccggca gcggatccgc cgatggcgcg ctgacgctgt tgcgcacgcg gccgctgctc
     3541 gcgcgcaagc gcgccgccgg ctactactgg ggcgtgggct tggtgtgggc gggcgacccc
     3601 gaggtcgtcg agttcgacgc caactcatgg gtaccgaccg gcatcgtcgg cggcagctgg
     3661 caggtcctgc ccgcctgggg cctcaaaggt cagctcgacg tgcacggcgc gttctacgac
     3721 tcggagctcg aggagatcgg ccagacggcc atcgaggcga cgttcagcgc gtggcggcga
     3781 atggggcggc gcgggcagct cgacctcgcc gtcgtcgagg acctgaacgt cagcaccgcg
     3841 cccgacgtcg tcttgcaagt cgccgcggcg tggcagtggt aagcccgttg ctgcggcttg
     3901 gcgccgcgct ctgcttgatg ctcgcagcct gcgattccgg ccgtcccggc gccgctgctc
     3961 gcacggagcc gccgccggcc actgccgtcg ctcgcgccgc cgggttcgat gcgggacccg
     4021 tcaagacggc ggcggaatac ctgctcgagc ccgagctcgc ctcggccgat gtcgcgcgcg
     4081 gcgaggtgct cggattcgcg tgcgcggcgt gccaccggtt tcgcgccgaa gagggcacgc
     4141 tgatcgggcc gaacttgcac ggcgtgttcg ggcgtcgcgc cgcgagcgtc gaggggttcg
     4201 cgtactcggc ggcgttgcgt gactcagggc tcgtctggac gccgcgctcg ctcgaggcgt
     4261 ggctcgcgaa tcccgccgat ttcgtcgagg gcacgacgat ggcgttcacg ggttatcgct
     4321 cggccgaaga ccggcgcgat ctgatcgcct atttgctgca cgcgacgcag tgagctcgtc
     4381 gaaaagcttt acacgcggcg cgtagagcgg gcccgagccg gtggcgcggc gcgaacgacc
     4441 gcccggcggc tcggttcttg catcgaaaac gccgtactca ctcgcactgg gtttcgagcg
     4501 tgaccgagcc tgtcgatcga gaggtctccg cggttcttcc cgccggcgtt tatgtcgagc
     4561 ggcgcagagg cggcgaccga cgtcgcttga cggtgcggag cttcctgcag ggcggcttca
     4621 ctccgcgtcg ccgcggcggc cggcgcgccg gcgagcagca tctgccgatc gactggcacg
     4681 agccgtactt gctgttcttg tcgctgacga tcctgctgct cagctttgcc gatgccttca
     4741 ttacgctgac gttgatcatg ggcggcgcac gggaagcgaa cccgctgctc gcctacatcc
     4801 tgcgcgatca ccccgagtgg ttcgccaccg tcaagatggg gctcaccgga gcgggcgtgc
     4861 tcatgctcgt cgcgatggcg cggtggcggc tgtttcgcat gcgcgtcggt ttgatgctgc
     4921 aagggatttt tgtggcctat gtcgcattga ttgcgtatga atggtggcta gtacgcacct
     4981 tgttgtgagc aatcgcgttt gaacttgaag gttgtttggc tacactacgc ccgccttcgg
     5041 gctcgggggc tgcataatga cggcgaaaga cccgtagcct ccgctcatga gcacagtatt
     5101 caagcaccag tacgatacgt cgcacttgca gggagccaac gcgacctacg tcgaatggct
     5161 ttatgagcag tacctcgccg agcccggcag cgtcccgcct gagtggcgcc aatatttcag
     5221 cacgctagcg gcgccgagcg ccgaggtccc gcacgggccg atcgttcaag ccgtggcgga
     5281 acgcactcgg cgcagcgctg ccgcgcccgc cgtcgcgccg gcggccggcg agggcgctcc
     5341 ggccatggag aagcaagcgg ccgtctcgcg cttgatccag gtctacagcc tgcgcggcca
     5401 tcaaatcgcc gacctcgacc cgctcggatt gtgggagcgt ccggcgccgg ccgtgctctc
     5461 gttcgatttc ttgggcctcg acgagtccga tctgcaacgc gaattttcca cgggcgggct
     5521 cgcgggcacg ggccatacgc gcatgaagct ccaggacatc ctggcgctct tgaagcacat
     5581 ctactgcgga aagctcgccg ccgtgttcgc gcacatctcg cgcgcgcgcg agcggctctg
     5641 g
//
LOCUS       DASSNN010000617         3939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1235887, whole genome shotgun sequence.
ACCESSION   DASSNN010000617 DASSNN010000000
VERSION     DASSNN010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235887"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VFL84_11510"
     CDS             <1..350
                     /locus_tag="VFL84_11510"
                     /inference="COORDINATES: protein motif:HMM:NF023358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3450 family protein"
                     /protein_id="HET7609296.1"
                     /translation="RITELMQNVQASPSEKFRRLMEAYQIEMEYGRTMTAYRQKLADG
                     RDAEMVRLGRVALLYRVMEDGETGYWDNERKAFVPDPDSAAAIEEALSIAKEERAPDL
                     IVVPVPAPQGVRS"
     gene            347..1846
                     /locus_tag="VFL84_11515"
     CDS             347..1846
                     /locus_tag="VFL84_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7609297.1"
                     /translation="MSGALKSYRRLVGALAFASLLAAMSVDAQQARPAQPQQAPAQQP
                     PAQPAPQQPQVQLGTLRQSVQRLRDQERALAQERTQRFRAALQREERRAQEAQQRRNA
                     AEARSNALDRQWNDNEKRIAETQALLTDKQGNLGELFGVTRQVAGDAATVLSQSLLTT
                     QYGTPTEGEDRGTFLRRVAAATALPSIGELERLWYEILREMTDSGKVAKYRTAVLLRD
                     DPDTADVDESQMAEEKEVVRVGPFTASSNGTYLGYQPSTKMLFELSGDLTAEFQELGR
                     NLQSTPPNADYQRALVDPARGGLLSRYIERPSWVTRVEQGEGVVYVIIAVGVLGVLLA
                     VFQGVYLLMTRLAVAAQLRNLDSPKANNPLGRVLIAFRGDGSKRVESAELAELRISEA
                     VLREMPRLERFQSFLRLAVAAGPLLGLIGTVIGMIITFHAIVASGTSDPRLMADGIGK
                     AMIATVAGLGIAIPLLFINAGLAALSRGVTQVLDEQSQALLARNALERA"
     gene            1964..2932
                     /locus_tag="VFL84_11520"
     CDS             1964..2932
                     /locus_tag="VFL84_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET7609298.1"
                     /translation="MIVGFAPEGGRLRAVGDSLGDLDRLVWIDLLNPTADEETTLEAQ
                     LGVDVPTREEMQRLEVSGRLYREDDALVMTAILPARTDSDDLLMAPVEFVLVGGKLVT
                     VRYHEPRAFKTFPQRAAESNVGCASGETVLVALLEATIDRMTDILERAEEEVDAISRD
                     IFRGEPQRSASRDLMKTLQLIGRKGDLCSNIQVSVLSLQRVTGFFGQELAERADGKEL
                     RRRVKTLEHYLQSLSGHASFTSQKVTFLLDATLGMINVEQSNIIKIFSIAAGVFLPPT
                     LIASIYGMNFAFLPELKWHYGYLLAWLLMLASALLPLWYFKRRGWF"
     gene            complement(2929..>3939)
                     /locus_tag="VFL84_11525"
     CDS             complement(2929..>3939)
                     /locus_tag="VFL84_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET7609299.1"
                     /translation="GAAAAVAAACTPSPLPPLHAVSGVAQGTTYSLQWVGGASEPEIA
                     AAAEQELARIDALLSNYRADSTLELFNGAATTEPVELPRELVALLETAKRIHEASDGC
                     FDPTVRPLVRAWGFDGDSPAIPEPAAIDAARAVVGFDKLDLVDATHARKTAAKLEIDM
                     ASIGQGYSAGRLGDVLERGGSTAYLAEIGGEVVARGAKPDGSAWRIGIENPGAGERGG
                     PALRMPPEMRTAVITSGSYRHYFDADGRRFGHIIDPRSGRPVEHSLVAVTVVGADPAT
                     AAGWATALLCLGPDAANEAAEREHLSALLWNAPEGEEPTLEQTPAFASEWSALLDEPR
                     PR"
BASE COUNT          640 a         1319 c         1352 g          628 t
ORIGIN      
        1 agcgcatcac ggagctcatg caaaacgtcc aggcctcgcc gtcggagaaa tttcgtcggc
       61 tcatggaggc gtatcagatc gagatggagt acggcagaac catgacggct taccgccaaa
      121 agctggccga cggccgcgac gcggagatgg tgcgcctcgg acgcgtcgcg ctgctgtatc
      181 gagtcatgga ggacggcgag accggctact gggacaacga acgcaaggcg ttcgtgcccg
      241 atccggattc ggccgccgcc atcgaagaag cgctgagcat cgccaaagag gagcgcgcgc
      301 cggatctcat cgtcgtgccg gttccggctc cgcaaggagt ccggtcatga gcggcgcatt
      361 gaagagctat cggcggctcg tcggagcatt ggccttcgct tcgttgctgg cggccatgtc
      421 ggtcgatgcc caacaagccc gaccggcgca accacagcag gcccccgcac agcaaccgcc
      481 cgcgcaaccg gccccgcagc aaccgcaggt tcagctcggc acgctgcgcc aatccgtgca
      541 gcgcctgcgc gatcaggaac gtgcgctggc gcaggagcgc acgcagcgct tccgcgcggc
      601 gctgcagcgc gaggagcgca gggcgcaaga ggcccagcaa cgccgtaacg ccgccgaggc
      661 ccgcagcaac gccctcgacc ggcagtggaa cgacaacgag aagcgcatcg ccgagacgca
      721 ggcgttgctg acggacaagc aaggcaatct cggcgagctc ttcggcgtca cgcggcaggt
      781 cgccggcgat gccgcgacgg tattgtctca gtcgctgctg acgactcagt acggcacacc
      841 gacggaaggc gaggatcgcg gcacgttctt gcggcgcgtg gccgccgcca cggcgctgcc
      901 gtcgatcggc gagctcgagc ggttgtggta cgagatcctt cgcgagatga ccgatagcgg
      961 caaggtcgcc aagtaccgca cggccgtctt gttgcgcgac gatccggaca cggctgacgt
     1021 cgacgagagt cagatggcgg aagagaaaga ggtcgtgcgc gtaggcccct tcaccgcgag
     1081 cagcaacggc acctacctcg gctatcagcc gagcacgaag atgttgttcg agctctccgg
     1141 cgatctcacc gccgagtttc aggagctcgg ccgcaacctg caatccacgc ccccgaacgc
     1201 cgattatcaa cgcgcgctcg tcgacccggc gagaggcgga ttgctgagcc gctatatcga
     1261 gcggccgagc tgggtgacgc gcgtcgagca gggcgagggc gtcgtctacg tgatcatcgc
     1321 cgtcggcgtc ctcggcgtgt tgctggccgt cttccaaggc gtctacttgc tcatgacgcg
     1381 cctggcggtc gccgcgcagc tgcgcaatct cgacagcccg aaggcaaaca atccgctcgg
     1441 ccgcgtcttg atcgcgttcc gcggcgacgg cagcaagcgc gtcgagagcg cggagctcgc
     1501 cgagctgcgg atctccgaag ccgtgctgcg cgagatgccg cggttggagc gcttccagtc
     1561 gttcctgcgg ctggccgtcg cggccggccc gttgctgggt ctcatcggca ccgtcatcgg
     1621 catgatcatc acgttccacg cgatcgtggc ttccggcacg agcgatccgc gcctgatggc
     1681 ggacggcatc ggcaaagcga tgatcgcgac ggtagccggc ctcggcatcg cgattccgct
     1741 gctgttcatc aacgcgggat tggcggcgct gtcgcgcggc gtcactcagg tgctcgacga
     1801 gcagagccag gcgctgctcg cacggaacgc gctcgaacgg gcctgaggct tccaaggcgg
     1861 ggtcgctgct tgcggccccg ccgtgttccc ttccgtcctt ccttcgcgcg cgccgcatag
     1921 cgcgtgctag agtcccgcgc acgcaagcgt cacgggtacc tcgatgatcg ttggatttgc
     1981 gcccgaaggc ggccggctcc gcgcggtcgg agattccctg ggcgatctcg atcggctcgt
     2041 ctggatcgac ctcttgaatc ccacggccga cgaggagacg acgctcgagg cacagctcgg
     2101 cgtcgacgtg ccgacgcgcg aagagatgca gcggctcgag gtatcgggcc ggctttacag
     2161 agaagacgac gccctcgtca tgacggcgat cctgccggcg cgcacggaca gcgacgatct
     2221 cctgatggct ccggtcgagt tcgtcttggt cggcggcaag ctcgtgacgg tgcgctatca
     2281 cgagccgcgc gcgttcaaaa cgttcccgca gcgggcggcc gagtcgaacg tcggctgcgc
     2341 gagcggcgag accgttctcg tcgcgttgct cgaagcgacg atcgatcgca tgacggacat
     2401 tctcgaacga gcggaggaag aggtcgacgc gatctcgcgc gacatcttcc gcggcgagcc
     2461 gcagagatcg gcgagccgcg acctgatgaa gacgctgcag ttgatcggcc gcaagggcga
     2521 cttgtgctcg aatatccagg tcagcgtgct gtcgctgcaa cgcgtgacag gcttcttcgg
     2581 tcaagagctc gccgagagag ccgatggcaa ggagctacgc aggcgcgtca agacgctcga
     2641 gcactatctg cagtccttga gcggtcacgc ttcgttcacg tcgcaaaagg tcacgttctt
     2701 gctcgacgcg acgctcggca tgatcaacgt cgagcagagc aacatcatca agatcttctc
     2761 gatcgcggcc ggcgtatttt tgccgcccac gttgatcgcg tcgatttacg gcatgaactt
     2821 cgcgttcctg ccggagctca agtggcacta cggctacctg ctcgcctggc tgctcatgct
     2881 cgcgtcggcg ctgctgccgc tctggtactt caagcgccgc ggctggttct agcgggggcg
     2941 cggctcgtcg agcagcgcgc tccactccga ggcgaaagcc ggagtctgct cgagcgtggg
     3001 ctcttcgccc tcgggcgcgt tccacagcag ggccgagagg tgctcgcgct ctgcggcctc
     3061 gttcgcggca tccgggccga ggcacagtag cgccgtggcc cacccggcgg ccgtcgcggg
     3121 gtcggcgccg acgaccgtga ccgctaccag cgagtgctcg acgggccggc cgctgcgtgg
     3181 gtcgatgatg tggccgaacc ggcgcccgtc ggcgtcgaag tagtgacggt aagagccgct
     3241 cgtgatcacc gcagttctca tttccggcgg cattcggagc gccgggccgc cgcgttcgcc
     3301 ggcgccggga ttctcgatgc cgatgcgcca cgcggagccg tcgggcttcg caccgcgcgc
     3361 tacgacctcg ccgccgatct cggcgagata ggccgtgctg ccgccgcgct ccagcacgtc
     3421 gccgaggcgg ccggccgagt agccctggcc gatgctcgcc atgtcgatct cgagcttcgc
     3481 ggcggtcttg cgcgcgtgcg tcgcgtcgac gagatcgagc ttgtcgaagc cgacgaccgc
     3541 gcgcgcggcg tcgattgccg caggctccgg aatggccggc gaatcgccgt cgaagcccca
     3601 ggcgcgcacg agcggtcgga ccgtcggatc gaagcagccg tcgctcgcct cgtggatgcg
     3661 ctttgccgtt tcgagcagcg cgacgagctc gcggggcagc tcgaccggct ccgtggtcgc
     3721 ggcgccgttg aatagctcga gcgtcgagtc cgcgcgatag ttcgacagga gcgcatcgat
     3781 tcgcgcgagc tcctgctccg cggccgccgc gatctccggc tcgctggcgc cgcccaccca
     3841 ttgcagcgag tacgtcgtgc cttgcgcgac gccggacacg gcatgcagcg gcggaagcgg
     3901 cgaaggcgtg cacgcggcgg ccacggcagc ggctgcgcc
//
LOCUS       DASSNN010000618        10776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_481894, whole genome shotgun sequence.
ACCESSION   DASSNN010000618 DASSNN010000000
VERSION     DASSNN010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10776
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481894"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2020)
                     /locus_tag="VFL84_11530"
     CDS             complement(<1..2020)
                     /locus_tag="VFL84_11530"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HET7609300.1"
                     /translation="MNVRDWTARIAVALVASALAACSGSSGDSGPPGDPTPGQQKLVS
                     TPTELLALEERPSCSAFNEHLTNAVTGLFVNGGFVGCRDCLVTLAGGAAVQASLGTDQ
                     QSFDRFTETNNQEAGVDELDHIDTDANGYFYLIDGTQLVVANGLPPTDLRAVAKLDLG
                     WRADGLVLDPANRRLVIVRSELGFLSLAQWISPPTYPEPVTELVFVDVADPAAPVIER
                     RLKIEGFKLAVRRIGSRVHLITHMTPFMPLPLVDDAELESLREHLADLPADGQGALRI
                     KAQIRARVAALVAGTDPFDFLPNVEVSTGSQAFTDITPANCGGVAIPKVASRLALTTV
                     TSVDSDGTDASSLTVASNSWNVYASETNVYLSQTSGSWWFDPNQRQQTVVYKIGIGAE
                     RPAYRAVGAVDGWADSSFQFSEHNGFLRIATHRADLAPDLAQRVEDNHVWVLGDDGAG
                     RLNVVGKVNGFAPGERIYSARFLGTRGFVVTFRRVDPLFTFDLSVPQDPRLVGQVEIP
                     GVSTYVHPLDAAHLLTIGYDGDQNRLNGQFQLQIFDVQNLAEPRLLHKLVPRFDAAGF
                     AWTAAVHDHLAFNYFPEGGTLTVPVQYFATSTTDHFSGFVAFSVSAANGFSELGRLDH
                     SDLARTEHCKSPSGVAPQACGSPIYLQAAQPRRAVSAQLAGATYIYTLS"
     gene            complement(2055..3071)
                     /locus_tag="VFL84_11535"
     CDS             complement(2055..3071)
                     /locus_tag="VFL84_11535"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HET7609301.1"
                     /translation="MRASYKPVVTKLAIPLLVASCGIDQGGYHPPEVLSSPTAQTVLV
                     SGPIDAFGSLRVNGLVLDTSRAQVRIDGRAASEADLRVGQMIRAVARVDGTAAAALSI
                     DYEENVVGPVAAVDAGAGEFTVLGQRVRATATTRFDGGRLNRFGDLRVGDRVAVSGMT
                     LPDDTVLATYVGRAAAAEPFEVTTVVTAVGPAATTFALGALTIDYSRANVLELPAGQP
                     IANTVVEVTGSELRDGVLVADYVRALPLLPGTFTAAATALTAAELPTAMSSASSPVAA
                     RFLGVVTGASADVLVFGDLEVSLTPATVVSGDRSSLVAGARVIVAGRILAPGRIEATR
                     IEIQ"
     gene            complement(3076..3906)
                     /locus_tag="VFL84_11540"
     CDS             complement(3076..3906)
                     /locus_tag="VFL84_11540"
                     /inference="COORDINATES: protein motif:HMM:NF039473.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HET7609302.1"
                     /translation="MEDSLRSIALAACRAALKPLVRILLKHGVMHREFVELSKQVYVE
                     IARDDYGIRGRPTNVARMALLTGMDRKEVTRVKTALERRADDLASSGPQQGQDRITRV
                     LSGWHQDREFIRDGKPLALPLEGPAPSFAALVKRYGGDVPATTILRELKRTGAARVAD
                     GTVHVLRRNYRLDTVDAAALSRAGSVLRDIGQTVTHNLYRAAREESRFEARATNLQIP
                     PWALPAYRKFVYAEGQAFLEKIDAWLTAAEIPEQEKAGSGAQRIGVGLYWIQSGEATE
                     "
     gene            complement(4031..4294)
                     /locus_tag="VFL84_11545"
     CDS             complement(4031..4294)
                     /locus_tag="VFL84_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609303.1"
                     /translation="MHDITADLLRSGGSLWSTVECDVRSDRVIVYARLRANHTHGVEG
                     ALVESERIFNSVLAGREWLAAVHWTDRLCRTFKSRPSEDDAPS"
     gene            complement(4310..5347)
                     /locus_tag="VFL84_11550"
     CDS             complement(4310..5347)
                     /locus_tag="VFL84_11550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET7609304.1"
                     /translation="MCLWPLLPAWWPAWLAAGLALAATQWRARGVAFRPQGMHAAGEH
                     RPSVVVIALASGIFWGATSVVFYPSGSVEHQLLLAFILTVITAAWLPLYAFVRMSLLA
                     FAVPAVLPMAFELLSSPTAPQTTMGSLLVLVIAALATIARHAGRWLHADSAVRQALYH
                     QATHDPLVGLANRAEFHRRAQTLEIMQANAYSIAFIDLDHFKPINDSLGHASGDALLR
                     SVGAVLRQTVRKGDTAARLGGDEFAILMKDCCAAEAAAVAAIILERIGALGRELGGGA
                     RPRVTASIGIACSADLCLSPATLLEAADQACYRAKRAGRNRIEIATELPKASARSRNR
                     ANVPVAAGPSL"
     gene            complement(5583..6269)
                     /locus_tag="VFL84_11555"
     CDS             complement(5583..6269)
                     /locus_tag="VFL84_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609305.1"
                     /translation="MDRQHIRDAQVAERYLNGTLSPAEEQAFELAYLADPELQEELDL
                     TERMRAAFKELGAAGPLERPARPRARWLDFVASPRYALAASLVAAVALVSSSALYVQN
                     QGLRDGASAASPAQTRLLSLVSVRGAANPNEIAAPAPDEWTVLLLDPGFGEHDVYRAV
                     LVQRDGREVLRRDGLTRTYEGLVALGMPGATLAPGAYEIRLSGGRRDWPATRELDDLS
                     RTPLNVAARP"
     gene            complement(6316..6975)
                     /locus_tag="VFL84_11560"
     CDS             complement(6316..6975)
                     /locus_tag="VFL84_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7609306.1"
                     /translation="MTTRGSISAGTAFFGELQRVVAEDSESEPQIAADLCARIHNGSR
                     DAEDQMVRRYGAGLLRLLKLRTRDAELALDLRQDTLRVAIERLRRSMLEDPTGLAAYL
                     RSVALNLLIAQRRKDTRRATAPDSIAIENAADERGGPFDNVSREQLRHVVGVLLNELN
                     TPRDRDLLTRFYIRDEDKATICAALSVDSVHFNRVLFRAKQRFRELLLQAERRSRPVV
                     G"
     gene            complement(6959..8635)
                     /locus_tag="VFL84_11565"
     CDS             complement(6959..8635)
                     /locus_tag="VFL84_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7609307.1"
                     /translation="MLRSTLALSLVTACAAARGAGVDGAVPVATDRVIVRVASQQAWP
                     DLEADLERADARLEAILTAAPLRVLIRMRSPAAGARIAAELEASGRVAYAVPETFRPM
                     ELRGRHVPNDPLFAAQWALDNADRTERPAGGDVDAPRAWGYTLGSPAVTIAVLDDGVQ
                     LDHPDLEPNIASAGRDFTVYPPAEGAGPRVASDRHGTSVAGIAAARGDNGLGVSGICP
                     RCRILPIRVHGSSNIGVAEAFRYAVAQGADVITNSWGYAPSGRRHGAAASVDAAVRDA
                     IDSAAREGRGGRGTVIVFGMTNEPVDNCGAHPDISALESVVAVGVSDRDDRIGGSGFG
                     ECMDIVAPAKPEGLRTAGSTTTDRTGIDGHAEGDYFDGFGGTSAAAPLVAGVAALLLS
                     LNPELTRDDVQRILEHTADKIDRAHAAYDAAGFSVRAGHGRVNAARALVPSVEITVTP
                     ARVAAGEPFSVTVAASAPFGLKSVAWRGAGTRDAALDVLREAPLGGEAYRSITWRDLT
                     IERPGTYAFEAFAEDLLAAEHAPGYPHRDGTDADELASLTVVERADDDSR"
     gene            complement(8644..9177)
                     /locus_tag="VFL84_11570"
     CDS             complement(8644..9177)
                     /locus_tag="VFL84_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609308.1"
                     /translation="MRVNAMRFSFVLLTAAVFAAGCARLTPDPRNPAPVKDSPQLLRV
                     CYVIGDAAHLAVGDQVELSTSGQGRLRIRHIPREGNNEIWNGGEAVAVKAAILVEAVP
                     GKQGARRFVPVGRFSVRVPDQGDAVHDEFDFATSHATANLQGSRFPECNVALGTDEVI
                     IRGVTDHDRHDGDAVLR"
     gene            complement(9174..>10776)
                     /locus_tag="VFL84_11575"
     CDS             complement(9174..>10776)
                     /locus_tag="VFL84_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET7609309.1"
                     /translation="LRVADPALRATFASARREYYETQIDLLMARAREARTTAEERDAR
                     SRAALEVSERGRARMIADLLQEAAVDLRSGAPADVRASEDRLYEQLADLRRRRDLLLE
                     RPAAPEDAAELERVVAELADTENALNLVEIELRRSNPRHATLTTPTPLTAAQIQRAVG
                     PDTVLLQYSLGEAASWVWVVTAERIAAVELPGRAAIEAAAQRALARLEVHAPDGVSRS
                     SAELEALAELVLAPVRESLNRPRVALALDGALQFVPFAVLPLPGADGVVAPLLAQHEV
                     VEVPSMSALALAPRDAVPPRRKTLAVFADPVLEPSDPRFGAARAETVAAAAREHGVAA
                     RSSAGVELGRLLSTGYEGETIAALVPDSARFLARGFAANRAAVLRSDLREFRYIHFAT
                     HGLVDARYPGLSALVLSQFDEQGRPLDGFLRLNDIYNLKLGADLVVLSACNTALGRDV
                     RGEGLIGLTQGFMTAGARSVVASLWQVPDSATAELMRQFYGYMLDEGLAPPAALRKAQ
                     LASAAQRRWSDPYFWGGFVLVGDWR"
BASE COUNT         1686 a         3766 c         3724 g         1600 t
ORIGIN      
        1 ccgaaagcgt gtagatgtag gtcgcgccgg cgagctgcgc cgacaccgcg cggcgcggtt
       61 gcgcggcttg cagatagatg gggctgccgc aggcttgagg cgccacgccg ctcggcgact
      121 tgcagtgctc ggtccgcgcg aggtcgctat ggtcgagtcg cccgagctcc gagaaaccgt
      181 tcgccgcgct gaccgagaac gcgacgaaac cgctgaagtg atcggtcgtg ctcgtggcga
      241 agtattgaac cggcactgtc agcgtgccgc cttcggggaa gtagttgaac gccaggtggt
      301 cgtgcacggc ggccgtccaa gcgaaaccgg cggcatcgaa ccgcgggacc agcttgtgca
      361 gcaagcgcgg ctccgcgaga ttttgcacgt cgaagatctg cagctgaaac tgcccgttga
      421 ggcggttctg atcgccgtcg tagccgatcg tcagcaaatg cgcggcgtcg agcgggtgca
      481 catacgtgct tacacccggg atctcgacct gcccgaccag ccgcgggtcc tgcggcaccg
      541 acaggtcgaa cgtgaacagc ggatcgactc gccggaacgt gacgacgaac cctcgcgtgc
      601 cgagaaagcg cgcgctataa atgcgctcgc cgggcgcgaa gccgttgacc ttgccgacga
      661 cattgaggcg gcccgcgcca tcgtcgccga gcacccacac atgattgtct tcgacccttt
      721 gtgccagatc gggcgcgagg tccgcgcggt gcgtcgcgat gcgcaggaac ccgttgtgct
      781 cgctgaactg gaagctcgag tcagcccaac cgtcgaccgc gccgacggcg cgatacgcgg
      841 ggcgctccgc cccgatcccg atcttgtaga cgaccgtctg ttgccgctgg ttgggatcga
      901 accaccaaga tccgctcgtc tgactgaggt agacgttcgt ctcgctcgcg tacacgttcc
      961 acgagttgct ggcgaccgtc agagacgacg catcggtgcc gtcgctgtcg acgctggtga
     1021 cggtcgtcag cgccagtcgc gaggccacct ttggaatcgc gacgcctccg caattcgccg
     1081 gcgtgatgtc cgtgaacgcc tgactgccgg tgctgacctc gacattgggc aggaagtcga
     1141 aaggatccgt gcccgccacg agcgccgcga cgcgcgcacg gatctgcgcc ttgattcgca
     1201 acgcgccctg gccgtcggcc ggcaggtcgg cgaggtgctc gcgcaagctc tcgagctcgg
     1261 cgtcgtcgac gagcggcaac ggcatgaacg gcgtcatgtg cgtgatgaga tgcacgcggc
     1321 tgccgatgcg ccgcacggcg agcttgaacc cttctatctt gagacgccgc tcgatcacgg
     1381 gcgccgccgg atctgcaacg tcgacgaaca cgagctccgt caccggttcc ggatacgtcg
     1441 gcggcgaaat ccactgcgcc aagctcaaga agccgagctc ggagcgcacg atgacgagcc
     1501 gccgattcgc ggggtcgagc acgagtccgt ctgcgcgcca accgagatcg agcttcgcga
     1561 cggcgcgcag gtcggtcggc ggcagaccgt tggcaacgac gagctgtgtg ccgtcgatca
     1621 gatagaagta gccattggcg tccgtgtcga tgtgatcgag ctcgtcgacg cccgcttctt
     1681 ggttgttggt ttccgtgaac cgatcgaatg attgctgatc ggtcccgagc gaggcctgca
     1741 ccgccgcccc gcccgcgaga gtgacgaggc agtctcggca accgacgaag ccgccgttca
     1801 cgaacaagcc cgtgaccgcg ttcgtaagat gctcattgaa cgcgctgcag cttggccgct
     1861 cctcgagcgc gagcagctcc gtcggcgtcg aaacgagctt ctgctgcccg ggagtagggt
     1921 ctcccggcgg gccgctgtcg ccggacgatc cgctgcaagc ggcgagcgcg ctcgccacga
     1981 gagcgactgc gatgcgtgcc gtccaatcgc gaacattcat gacgcacctc ccttgaagtt
     2041 cttccgtgct cgagtcactg aatttcgatg cgcgtcgctt cgatgcgccc cggcgcgagg
     2101 attcggccgg cgacgatgac gcgcgcgccc gcaacgaggc tcgagcgatc gccgctcacg
     2161 acagtcgctg gcgtcaagct cacctcgaga tcgccgaaga cgagcacgtc ggcgctcgcg
     2221 cccgtcacga cgccgaggaa gcgagccgcg acgggcgacg aggcggacga catcgccgtg
     2281 ggcagctcgg cagcggtcag cgcggttgcg gcggccgtaa acgtgccggg caggagcggc
     2341 agcgcgcgga catagtcggc gaccaacacg ccgtcgcgga gctccgagcc ggtcacctcg
     2401 acgacggtgt tcgcgatcgg ctggccggcc ggcaactcga ggacgttcgc gcgcgagtag
     2461 tcgatcgtca gcgcgccgag cgcgaacgtc gttgccgccg gcccgacggc cgtgacgacg
     2521 gtcgtgacct cgaacggctc tgccgccgcg gcgcgcccga cgtacgtcgc gagcacggtg
     2581 tcgtccggca acgtcatacc gctgacggcg acgcgatcgc cgactcgcag atcgccgaag
     2641 cggttgagcc gcccgccgtc gaatcgcgtc gtggccgtgg cgcgcacgcg ctgcccgagc
     2701 accgtgaatt cgccggcgcc ggcatcgacg gcggcgacgg gacccacgac gttctcctcg
     2761 tagtcgatcg agagcgccgc cgccgccgtg ccgtcgacgc gcgctaccgc ccggatcatc
     2821 tggccgacgc gcaagtcggc ttcgctcgcc gcgcggccgt cgatgcgtac ttgcgcgcgc
     2881 gaagtgtcga gcacgaggcc gttgacgcgc aggctgccga acgcgtcgat tggcccggag
     2941 acgagcaccg tttgcgccgt tggactcgag agcacctcgg gcgggtggta gccgccctgg
     3001 tcgatgccgc aacttgcgac gagcaacggg atggcgagct tcgtcacgac gggcttgtat
     3061 gaggctctca tgcgctcact ccgtagcttc gcccgactga atccaataaa gacccacgcc
     3121 gatccgctgc gcgccgctcc cggctttttc ttgctccgga atttccgcgg ccgttagcca
     3181 cgcatcgatc ttctcgagga aagcctgccc ttccgcgtag acgaacttgc ggtaggccgg
     3241 cagtgcccac ggcggaatct gcaagttcgt cgctcgcgct tcgaagcggc tttcttcgcg
     3301 cgcagcgcgg tagaggttgt gcgtgacggt ctgaccgatg tcgcgcagca cgctgccggc
     3361 gcgcgacagc gcggcggcat ccacggtgtc gagcctatag ttgcggcgca gcacgtgcac
     3421 ggtgccgtcc gcgacgcgcg cggctcccgt gcgcttcagc tcgcggagaa tcgtcgtggc
     3481 gggcacgtcg ccgccgtagc gcttgacgag cgccgcgaag ctcggtgccg gaccttcgag
     3541 cggcagcgcg agcggctttc cgtcgcggat gaactctcga tcttgatgcc agcccgagag
     3601 cacgcgcgtg atgcggtcct gaccttgctg cggacccgac gacgcgaggt cgtcggcgcg
     3661 ccgctcgagc gcggtcttca cgcgcgtgac ttcttttcga tccatgccgg tcaggagcgc
     3721 catgcgcgcg acgttcgtgg gccgcccgcg gatgccgtag tcgtcgcgtg caatctcgac
     3781 gtacacctgc ttactgagct ccacgaattc ccggtgcatg acgccgtgct tgagcagaat
     3841 ccgcaccagc ggcttcaagg cggcccgaca cgccgccagc gcgatggagc ggaggctgtc
     3901 ttccatatgt gaggctttta cccccatata tggccatccg tcaagaaaaa tcggtccgag
     3961 ccgccgcgac cggcgcgcgg ccggcgacgg cggccgatcc gatgcgcggg ctagcgcaga
     4021 ccgcgcggcg tcaagagggg gcgtcgtctt cggaaggacg cgacttgaaa gtgcggcaca
     4081 agcgatccgt ccagtgcacg gctgccagcc attcgcggcc ggcgagcacc gagttgaaga
     4141 tccgttccga ttcgacgagc gcgccttcga cgccgtgcgt gtggttcgcc ctcagccgcg
     4201 catagacgat cacgcgatcg ctcctgacgt cgcactcgac tgtcgaccat agcgagccgc
     4261 cgctccggag caggtctgcc gtgatgtcgt gcatgtcgat tcgcgcgcgc tagagcgagg
     4321 gcccggccgc cactggcacg ttcgcgcgat tgcgggaccg cgcactggcc ttcgggagct
     4381 cggtggcgat ctcgatgcga ttgcggcccg cgcgtttcgc gcggtagcac gcttggtcgg
     4441 cggcttcgag cagtgtcgcg gggctcaagc acaggtcggc gctgcaagcg atgccgatgc
     4501 tcgccgtgac acgcggacgc gcgccgccgc cgagctcgcg gccgagcgct ccgatgcgct
     4561 cgaggatgat cgcagccacg gcggccgcct ctgccgcgca gcaatccttc atgagaatcg
     4621 cgaactcatc gcctccgagc cgcgcggccg tatcgccttt gcgcacggtt tggcgcagca
     4681 ccgctccgac actgcgcagt agcgcatcgc ccgacgcatg tcccagcgag tcgttgatcg
     4741 gcttgaagtg atcgagatcg atgaacgcga tcgagtacgc attcgcctgc atgatctcga
     4801 gcgtctgcgc gcgccgatgg aactcggcgc gattcgccaa gcccacgagc ggatcgtgcg
     4861 tggcctggtg atacagcgcc tggcggaccg cggaatcggc gtgcagccat cggccggcgt
     4921 gccgcgcgat cgtcgcgagc gccgcgatga cgagcactaa aaggctgccc atggtcgttt
     4981 gcggggcggt gggggatgac aggagctcaa acgccatggg cagcacagcc ggaacggcga
     5041 acgccagcag cgacatccgc acgaatgcgt aaagcggcag ccaagcggcc gtgatcaccg
     5101 tcaggatgaa ggcgagcagc aattgatgct cgaccgagcc gctcgggtag aacacgacgc
     5161 tcgtcgcgcc ccagaagatt cccgacgcga gcgcgatgac gaccaccgaa ggccgatgct
     5221 cgccggcggc gtgcatgcct tgcgggcgga aggcgacgcc gcgggcacgc cactgcgtcg
     5281 cagcgagcgc caatccggcc gcgagccatg ccggccacca cgcgggaagc aggggccaga
     5341 ggcacaagac gactgctagg cagccgcccg cggtgctgag cggcgcgacg gtagcgaaac
     5401 gccgcagatg gcgggcgcgt tgccaaccgt gcgcctcatc gccatcgacg aaactgtctg
     5461 ggtggtcagc gacgttcatt attgtttcct cgcctgccgt cgcattggcg gcacaccctg
     5521 tcagctgccg cgagtgcgcg aagcgtctca ccccgtcgcg aggttttttt cgaggacgag
     5581 cgttacgggc gcgcggctac gttcagcgga gtgcggctca ggtcgtccag ctcgcgcgta
     5641 gcgggccaat cgcggcggcc gcccgagaga cggatctcgt aggcgccggg cgccagggtc
     5701 gcacccggca tgccgagcgc gaccaggccc tcgtaggtcc gcgtgaggcc gtcacggcgc
     5761 agcacctcgc gtccgtcgcg ctgcacgagc actgcgcgat agacgtcgtg ctcgccgaaa
     5821 cccgggtcga gcaacagcac ggtccattcg tccggcgccg gcgcagcgat ctcgttcgga
     5881 ttggccgcgc cgcgcacgga gacgagagac aacaaccgag tctgagcggg cgacgccgcc
     5941 gaggcaccgt cgcgcaatcc ctgattctgc acatacaacg cgctgctcga cacgagcgcc
     6001 accgcggcga ccagcgacgc ggcgagcgcg tagcgcggcg atgccacgaa gtcgagccag
     6061 cgcgcgcgcg gccgggccgg gcgctcgagc ggtcccgcgg cgccgagctc cttgaacgcg
     6121 gcccgcattc gctcggtgag atcgagctct tcctgcagct ccgggtcggc gaggtaggcc
     6181 agctcgaacg cctgctcctc ggccggagat agcgtcccgt tgagatagcg ctcggcgact
     6241 tgggcgtcgc gaatgtgctg tcgatccata tgagtcaggc tgtctccacc gcttcgttta
     6301 tctcacgcga cgcgcctagc cgacgaccgg ccggctgcgg cgctcggctt gaagcaacag
     6361 ctcgcgaaac ctctgtttcg cgcgaaacag cacgcgattg aagtgcacgg agtcgacgct
     6421 gagcgccgcg caaatcgtgg ctttgtcctc gtcgcggata tagaaccgcg tcaacaagtc
     6481 gcggtcccgc ggcgtgttga gctcgttcag cagcacgccg acgacgtggc gcagctgctc
     6541 gcgcgagacg ttgtcgaacg ggccgccccg ctcgtccgcc gcgttctcga tcgcgatcga
     6601 gtcgggcgcc gtcgcgcgcc tcgtgtcctt gcgccgctgt gcgatcaaga ggttgagggc
     6661 cacgctgcgc aagtacgcgg cgagacccgt tgggtcctcg agcatggacc ggcgcagcct
     6721 ctcgatcgcg acacgcagcg tgtcttgccg cagatcgagc gcgagctccg catcgcgcgt
     6781 gcgcagcttg agcagccgca gcaaacccgc gccgtagcgc cgcaccatct ggtcttcggc
     6841 atcgcgcgag ccgttgtgta tgcgcgcgca caaatctgcc gcaatttgcg gctccgactc
     6901 ggagtcctcg gccacgaccc gctgcagctc cccgaaaaac gcggtccccg ccgagatgct
     6961 accgcgagtc gtcatcggcg cgctcgacga ccgtcagcga ggccagctcg tcggcatccg
     7021 tgccgtcacg atgcggatag cccggcgcgt gctccgccgc gagcagatcc tccgcgaacg
     7081 cttcgaaggc gtaggtcccg ggtcgctcga tcgtcagatc gcgccaggtg atggagcggt
     7141 acgcctcgcc gccgagcggc gcctcgcgca agacgtcgag cgcggcatcg cgggtgccgg
     7201 cgccgcgcca tgcgacggat ttgagcccga acggcgcgct cgccgcgacc gtgacgctga
     7261 acggctcgcc cgccgcgacg cgcgccgggg ttacggtgat ctcgacgctc ggcaccagcg
     7321 cgcgcgccgc gttcacgcgg ccgtggccgg cgcgcacgct gaagcccgcg gcgtcgtagg
     7381 cggcgtgggc gcgatcgatc ttgtcggccg tatgctcgag gattcgctgg acgtcgtcgc
     7441 gcgtgagctc tgggttcagc gacagcagca acgcggccac gcccgcgacg agcggcgccg
     7501 ccgccgacgt tcccccgaag ccgtcgaaat agtcgccctc ggcatgaccg tcgattccgg
     7561 tccggtccgt cgtcgtgctg cccgccgtgc ggagcccctc cggtttcgcc ggcgcgacga
     7621 tgtccatgca ctcgccgaag cccgagccgc cgatgcggtc atcgcggtcc gagacaccga
     7681 ccgcgacgac gctctcgagc gccgagatat cggggtgcgc gccgcagttg tcgaccggct
     7741 cgttcgtcat gccgaacacg atgacggtgc cgcggccgcc gcggccctcg cgcgccgcgc
     7801 tgtcgatcgc gtcgcgcacg gcggcgtcga cgctcgcggc cgctccgtgc ctgcggccgc
     7861 tcggcgcata tccccagctg ttcgtgatga cgtccgcgcc ttgcgcgacc gcgtagcgaa
     7921 aagcctcggc gacaccgatg ttcgacgagc cgtgcacgcg aatcggcaag atccggcaac
     7981 gcgggcagat gcccgagact ccgaggccgt tgtcgccgcg cgcggccgcg atgccggcga
     8041 ccgaggtccc gtggcgatcg gaagcgacgc gcggcccggc accctcggcg ggcggataca
     8101 ccgtgaagtc gcgcccggcg ctcgcaatgt tcggctcgag gtccggatga tcgagctgca
     8161 cgccgtcgtc gagcacggcg atcgtcaccg ccggcgagcc gagcgtgtag ccccaggcgc
     8221 gcggagcgtc gacgtcgccg ccggccggac gctcggtccg gtccgcgttg tcgagcgccc
     8281 actgcgcggc gaacagcggg tcgttcggca cgtggcggcc gcgcagctcc atcggccgga
     8341 aagtctccgg caccgcgtat gcgacgcggc ccgacgcctc gagctcggct gcaatacgcg
     8401 cgccggcagc cggggaacgc atgcggatca gcacgcgcaa cggcgcggcg gtcaggatcg
     8461 cctcgagccg cgcgtcggcg cgctcgagat cggcctcgag atccggccac gcctgctgcg
     8521 aagcgacgcg cacgatgacg cggtcggtgg cgacgggcac ggcgccgtcg acgcccgcgc
     8581 cgcgcgccgc cgcgcacgcc gtcacgagcg agagcgcgag cgtcgaccgc agcatggctt
     8641 cgatcagcgc aagaccgcgt cgccgtcgtg ccggtcgtgg tccgtgacgc cgcggatgat
     8701 gacttcgtcg gtgccgagcg cgacgttgca ctccggaaag cggctgcctt gcaggttcgc
     8761 cgtcgcgtgg ctcgtcgcga agtcgaactc gtcgtgcacg gcatcgcctt gatccggaac
     8821 gcgcaccgag aaacgcccga ccggcacgaa gcggcgcgcg ccctgcttgc cgggcacggc
     8881 ctctacgagg atggccgcct tcacggcaac ggcttcgccg ccgttccaga tttcgttgtt
     8941 gccttcgcgc gggatgtgcc gaatgcgcag gcgcccctga ccgctcgtcg acagctccac
     9001 ttggtcgccg acggcgagat gcgccgcgtc gccgatgacg tagcagacgc gcagcagctg
     9061 cggcgaatct ttgaccggcg ccggattgcg cggatccggc gtcagccggg cgcaacccgc
     9121 cgcgaagacg gccgcggtca gcagcacgaa cgaaaaacgc atagcgttga ctctcatcgc
     9181 caatctccca ctagaacgaa cccaccccag aaatacggat cgctccagcg ccgctgcgcg
     9241 gcgctcgcaa gctgcgcctt gcgcagcgcg gccggcggcg cgagcccttc gtcgagcatg
     9301 tagccgtaga actgcctcat gagctcggcc gtcgcgctgt cgggcacctg ccaaaggctc
     9361 gcgacgacgc tgcgcgcgcc cgcggtcatg aagccctgcg tgagaccgat caggccctcg
     9421 ccgcgcacgt ccctgccgag cgccgtattg cacgcgctga gcacgacgag atccgcgccg
     9481 agcttcaaat tgtagatgtc gttgagccgc agaaacccgt cgagtggcct gccctgctcg
     9541 tcgaactgcg agagcacgag cgcggacaat cccggatagc gcgcatcgac gaggccgtgc
     9601 gtcgcgaagt gaatgtagcg gaactcgcgc aagtcggagc ggagaacggc cgcgcggttc
     9661 gcggcgaacc cgcgtgcgag gaatcgcgcc gagtccggca cgagcgccgc gatcgtctcg
     9721 ccttcgtagc ccgtcgagag cagccgcccg agctcgacgc cggccgagga gcgcgccgcg
     9781 accccgtgct cgcgcgctgc cgcggcgacc gtctcggctc gcgcggcacc gaaccgcggg
     9841 tcgctcggct cgagcacggg atcggcgaat acggcgagcg tcttgcgccg cggcggcacg
     9901 gcatcgcgcg gcgccagtgc aagcgccgac atcgaaggca cctcgacgac ctcgtgctgc
     9961 gcgagcagcg gcgcgacgac cccgtccgcg cctgggagcg gcaacaccgc gaacggcacg
    10021 aactgcaggg ccccgtcgag ggcgagcgcg acgcgcggcc gattgaggct ctcgcggacc
    10081 ggagcgagca cgagctcggc cagcgcctcg agctccgccg aagatcgcga gacgccgtcc
    10141 ggcgcgtgca cctcgaggcg cgccagcgcg cgctgcgcgg cggcctcgat cgccgcacgg
    10201 ccgggcagct cgaccgcggc aatgcgctcc gccgtcacga cccataccca gctcgcggcc
    10261 tcgccgagcg agtactggag cagcacggtg tccggcccca cggcccgctg gatctgcgcc
    10321 gcggtcagag gcgtcggcgt cgtcagcgtc gcgtgccgcg ggttgctgcg ccggagctcg
    10381 atctcgacga ggttcagcgc attctcggtg tccgcgagct ccgcgacgac gcgctcgagc
    10441 tcggccgcat cttcgggagc tgccgggcgc tcgagcagca ggtcgcgccg gcgtctcaag
    10501 tccgcgagct gctcgtagag ccggtcctcg ctcgcgcgga cgtcggcggg agcgccgctg
    10561 cgcagatcga cggcggcttc ttgaaggagg tcggcgatca tgcgagcgcg gccgcgctcg
    10621 ctgacctcga gggcggcgcg cgagcgcgcg tcacgctcct cggccgtcgt gcgcgcttcg
    10681 cgcgcgcgcg ccatcagcaa gtcgatctgc gtctcatagt actcgcgccg cgccgaggcg
    10741 aacgtggctc gcagcgccgg gtccgcgacg cgcaag
//
LOCUS       DASSNN010000619         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1489280, whole genome shotgun sequence.
ACCESSION   DASSNN010000619 DASSNN010000000
VERSION     DASSNN010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489280"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /gene="tsf"
                     /locus_tag="VFL84_11580"
     CDS             <1..849
                     /gene="tsf"
                     /locus_tag="VFL84_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018934992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET7609310.1"
                     /translation="ERTGAGMMECKKALVETKGDIDAAAEWLRKAGAAKADKKSSRVA
                     AEGRIAIRSDEKAGRHVVLEVNSETDFVAKDDNFRAFVERVADVILEQRPANVEALMR
                     SSVGGKTVEDLRLELVTKVGENISVRRFDVVDSRGNVGAYVHGTRIGVLVDVEGGSPD
                     LARDLAMQVASLAPRYVSNDDVPKDVVAKEREIIAAQTAEEKKPPEIVAKMVEGKLRK
                     FLDEITLTGQVFVKDDKKRVRDLLSQNKAKVKGFRRYEVGEGIEKKSVDYAKEVEAIA
                     QGSQSR"
     gene            1017..1766
                     /gene="pyrH"
                     /locus_tag="VFL84_11585"
     CDS             1017..1766
                     /gene="pyrH"
                     /locus_tag="VFL84_11585"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET7609311.1"
                     /translation="MAQRSPAYKRILLKLSGEALLGGQNYGIDPAVIRRVASEITEVV
                     GLGVQVGLVIGGGNIFRGAGLAEAGMDRVTADQMGMLATVINALAMQDALERAGTGVR
                     VMSALQIHQVCEDYIRRRAVRHLEKGRVTVFAAGTGNPFFTTDTAASLRAIEIGADVL
                     IKATKVDGVYSADPMKDPKAVRYPALSFDQVLSDDLRVMDATAIVMCRENNLPLVVFN
                     LNTPGDLVRLVTGEPVGTSVMNHLPRTGTGK"
     gene            1763..2320
                     /gene="frr"
                     /locus_tag="VFL84_11590"
     CDS             1763..2320
                     /gene="frr"
                     /locus_tag="VFL84_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET7609312.1"
                     /translation="MINDVKKGAEQRMQRGIEAFKEDLRKLRTGRAHAGLLEHVMVEY
                     YGTSVPVKQTANISVEDARTLVVTPWEKNMVSAVEKAIINSGLGLNPVTAGQVIRVPL
                     PPMTEERRRDLVKVVRAHAETARVALRNVRREALADIKDLLKEKMIAEDDERRGQEEI
                     QKLTDRYVATVDSLLKDKEAEIMEF"
     gene            2340..>2652
                     /locus_tag="VFL84_11595"
     CDS             2340..>2652
                     /locus_tag="VFL84_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609313.1"
                     /translation="MLAARIITGVLFGAALTAALLFAPSPVTAAVLGILWLAGVWEWG
                     AFAKLPAAGRAGYTLLFAAAMVVGGPWLGTKGLGVLLLAALAWWLFALVLVVRYPRTF
                     SP"
BASE COUNT          479 a          863 c          898 g          412 t
ORIGIN      
        1 gagcgcaccg gcgccggcat gatggaatgc aagaaggcgc tcgtcgaaac gaagggcgat
       61 atcgacgcgg cggccgagtg gctgcgcaag gccggcgccg cgaaagccga caagaagtcg
      121 tcgcgcgtcg cggccgaagg ccgcatcgcg attcgtagcg acgagaaggc cggccggcac
      181 gtcgtgctcg aggtcaactc ggagacggac ttcgtcgcga aggacgacaa cttccgcgcg
      241 ttcgtcgagc gcgtggccga cgtcatcctc gagcaacgcc cggcgaacgt cgaagcgctg
      301 atgcgcagct cggtcggcgg caagaccgtc gaggacttgc gtctcgagct cgtcacgaaa
      361 gtcggcgaga acatctccgt gcgccgcttc gacgtcgtcg acagccgcgg caacgtcggc
      421 gcgtacgttc acggcacgcg gatcggcgtg ctcgtcgacg tcgaaggcgg aagccccgat
      481 ctcgcgcgcg atctcgcgat gcaggttgcg tcgctggcgc cgcgctacgt ctcgaacgac
      541 gacgtgccga aggacgtcgt cgccaaggag cgcgaaatca tcgccgcgca gacggccgag
      601 gagaagaagc cgcccgagat cgtcgccaag atggtcgagg ggaagctgcg caagttcctc
      661 gacgagatca cgctgacggg ccaagtcttc gtcaaggacg acaagaagcg cgtccgcgac
      721 ctgctctcgc agaacaaggc caaggtcaaa ggtttccggc gctacgaggt cggcgagggc
      781 atcgagaaga aatccgtcga ctacgccaag gaagtcgagg ccatagcgca agggtcgcag
      841 agccgataat ctaacccgtc ccgtcccggc tgagcgctca cgcggagggg ccctcccgag
      901 agacgccgga agaccatccc tggggctcgt cttcgcacat cctaagtgct cagacgctct
      961 cgggagggcc ctccgcgcga gcgcccagcc gggacgcacg acagagcaga ggttttatgg
     1021 cgcaacgctc gccggcctat aagcggattc tgctgaagtt gagcggcgag gccctgctcg
     1081 gcggccaaaa ttacggcatc gatcccgccg tgattcgccg cgtcgcctcc gagatcaccg
     1141 aggtcgtcgg tctcggcgtc caggtcggcc tcgtgatcgg cggcggcaac atcttccgcg
     1201 gcgcaggcct cgccgaagcc ggcatggacc gcgtgaccgc cgatcagatg ggcatgctcg
     1261 ccaccgtcat caacgcgctc gcgatgcagg acgcgctcga gcgcgcaggc accggcgtgc
     1321 gcgtcatgtc ggcgctgcag attcaccagg tgtgcgagga ctatatccgc cggcgcgccg
     1381 tgcgccacct cgagaagggc cgcgtcaccg ttttcgccgc cggcaccggc aacccgttct
     1441 tcacgacgga taccgccgcg agcttgcgcg cgatcgagat cggcgccgac gtgctgatca
     1501 aagcgacgaa ggtcgacggc gtctactcgg ccgatccgat gaaggatccc aaagccgtgc
     1561 gctatccggc gctgtcgttc gatcaggttt tgtccgacga cctgcgcgtc atggacgcga
     1621 cggccatcgt catgtgccgc gagaacaacc tgccgctcgt cgtgttcaac ttgaacactc
     1681 cgggcgatct cgtgcgcttg gtgaccggcg agccggtcgg cacctcggtc atgaaccact
     1741 tgccgcgtac ggggactgga aaatgatcaa cgacgtcaag aagggcgccg agcagcgcat
     1801 gcagcgcggc atcgaggcgt tcaaggaaga cctgcgcaag cttcgcacgg gccgcgcgca
     1861 cgcgggcctg ctcgagcacg tcatggtcga atattacggc acgagcgtgc ccgtgaagca
     1921 gacggccaac atctccgtcg aggacgcgcg cacgctggtc gtgacgccgt gggaaaagaa
     1981 catggtgtcg gccgtcgaga aggcgatcat caactccggg ctcggcttga atcccgtgac
     2041 ggccggccag gtcattcgcg tgccgttgcc gccgatgacg gaggagcggc gccgcgatct
     2101 cgtcaaagtc gtccgcgcgc acgccgagac ggcccgtgtc gcgctgcgca acgtgcggcg
     2161 cgaagcgctc gccgacatca aggacttgct caaggaaaag atgatcgccg aggacgacga
     2221 gcgccgcggc caagaggaga ttcagaagct caccgatcgc tatgtcgcca ccgtcgattc
     2281 gcttctgaaa gacaaggaag ccgagatcat ggagttctga gcgcccgctc acgctcgtca
     2341 tgctggcagc gcgcatcatc acgggcgtcc ttttcggcgc cgccctcacc gccgcgctgc
     2401 tgttcgcgcc gtctccggtc acggctgccg tcttgggcat tctctggctg gccggggttt
     2461 gggaatgggg agcgttcgcg aagctgccgg ccgcgggacg cgccggctac acgcttctgt
     2521 tcgctgccgc gatggtggtc ggcgggccgt ggctcgggac caagggcctc ggcgtgctgc
     2581 tgctcgcggc gctcgcgtgg tggctgtttg cgctcgtgct cgtcgtgcgt tatccgcgca
     2641 cgttcagccc aa
//
LOCUS       DASSNN010000620         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1489392, whole genome shotgun sequence.
ACCESSION   DASSNN010000620 DASSNN010000000
VERSION     DASSNN010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489392"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..956
                     /locus_tag="VFL84_11600"
     CDS             3..956
                     /locus_tag="VFL84_11600"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET7609314.1"
                     /translation="MDPISTTIFSGNAHPALARDIAYHLGLSLGRISVGRFSDGEISV
                     EIMENVRGRDVFIVQPTCPPVNENLMELLIMADACQRASAARVTAVIPYFGYGRQDRR
                     RRAARVPITGKLVANLIATAGIDRVLFVDVHADQITGFFDIPVDNVYASPVLLGDAWK
                     QKYKDMVVVSPDVGGVERARALAKRLDDAGLAIIDKRRPQPNMSEVMHVIGEVAGKTC
                     VLIDDMVDTAGTLCQAADVLKQRGAVRVVAYITHPVLSGPAVERIKASALDEIVVTDT
                     IPLNEAARACKRIRQLSVAELLAETLRRINESDSVSSLYLD"
     gene            1011..1775
                     /locus_tag="VFL84_11605"
     CDS             1011..1775
                     /locus_tag="VFL84_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006955202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HET7609315.1"
                     /translation="MKAVFELAGEPRATLGRTNSRRLRKAGKVPAIMYGGGEAPEALV
                     LEHNALKNQMSKEAFYTSILTLKIGSKAQQVVVKGVERHPARPQIMHMDFQRVREDVE
                     ITLTVPIHFTGEAEAKGVKEQGGVVDHLMTDVEVRCLPRYLPEFLTLDVTALELNQIY
                     HLSDIKLPEGVTLVALAGGNDHPVVAVNLPRAEEIDIPAETETPAGEVPALAQAEPGK
                     EGEAAAAAGGDAAKADAKAGGAKPEAKGGGKPEGKK"
     gene            1917..>2423
                     /gene="pth"
                     /locus_tag="VFL84_11610"
     CDS             1917..>2423
                     /gene="pth"
                     /locus_tag="VFL84_11610"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET7609316.1"
                     /translation="MGTPIDLIVGLGNPGPEYAATRHNAGFWFVDLLAKEHGGSFSRE
                     RKLQGEAAEIAIAGKRIRLLKPTTYMNLSGQSVGAAVNYYKIPTENVLVAYDELDLPA
                     GRMQLRFDGSSAGHNGINSVIEHIGSKFWRLRFGIGHPRDGEHASGERKDVIDHVLKR
                     AGGDEEDLL"
BASE COUNT          452 a          806 c          789 g          376 t
ORIGIN      
        1 cgttggaccc tatttcgacg accattttct ccggcaacgc tcaccccgcg ctggctcgcg
       61 acatcgcgta tcacttgggc ctatcgctcg gccgcatttc cgtcggccgc tttagcgacg
      121 gggagatcag cgtcgagatc atggagaacg tgcgcggccg cgacgttttc atcgtgcagc
      181 cgacgtgccc gcccgtgaac gagaacctca tggagcttct gatcatggcc gacgcgtgcc
      241 agcgggcttc cgccgcgcgt gtcaccgccg tgattcccta cttcggctac ggccgccagg
      301 accggcggcg ccgcgcggca cgcgtgccga tcacgggcaa gctcgtcgcg aacttgatcg
      361 cgacggccgg catcgaccgc gtgctgttcg tcgacgtgca cgccgatcag atcacgggct
      421 tcttcgacat tcccgtcgac aacgtctacg cgtctccggt cttgctcggc gacgcgtgga
      481 agcagaaata caaggacatg gtcgtcgtat cgccggacgt cggcggcgtc gagcgcgccc
      541 gcgcgctcgc gaagcggctc gacgacgcgg gcctcgcgat catcgacaaa cggcgcccgc
      601 agccgaacat gtccgaggtc atgcacgtga tcggcgaggt cgcgggcaag acctgcgtgc
      661 tgatcgacga catggtcgac actgccggca cgctgtgcca agcggccgac gtattgaagc
      721 aacgcggcgc cgtccgcgtc gtggcctata tcacccaccc cgtcctctcg gggcccgccg
      781 tggagcggat aaaagcctcg gcgctcgacg agatcgtcgt cacggacacg atcccgctga
      841 acgaggcggc ccgggcctgc aagcggattc ggcagctgtc agtggccgag ctgctggccg
      901 aaaccctgcg tagaatcaac gagtcggact cggtcagctc gctctatctc gactagctta
      961 ggctccgttg gccctgctaa gatgcgcggc ttcctggagg taccctagcc gtgaaagccg
     1021 tattcgaact ggccggagag ccacgcgcca cgctcggccg caccaatagc cgccgcctgc
     1081 gcaaggccgg caaagtcccc gccatcatgt atggcggcgg cgaagcgccc gaggcgctcg
     1141 ttctcgagca caacgcgctc aagaatcaga tgagcaagga agcgttctac acgagcatct
     1201 tgacgctcaa gatcggctcg aaggcgcagc aggtcgtcgt caagggcgtc gagcgccatc
     1261 ccgctcggcc gcagatcatg cacatggact tccagcgcgt gcgcgaggac gtcgagatca
     1321 cgctgaccgt gccgatccac ttcacgggcg aggccgaggc gaagggcgtc aaggaacagg
     1381 gcggcgtcgt cgaccacctg atgaccgacg tcgaagtccg ctgcttgccg cgctacctgc
     1441 ccgagtttct gacgctcgac gtcacggcgc tcgagctgaa tcagatctat cacctctccg
     1501 acatcaagct gccggagggc gtcacgctcg tcgcgctcgc cggcggcaac gatcaccccg
     1561 tcgtcgcggt caacttgccg cgcgccgagg aaatcgacat tccggccgag accgagacgc
     1621 cggccggcga ggttcccgcg ctggcgcagg ccgagcccgg caaggaaggc gaagcggctg
     1681 cggccgccgg cggcgacgcc gcgaaggccg acgccaaggc cggtggcgcc aagcccgagg
     1741 ccaaaggcgg cggcaaaccc gaaggcaaga aataagagtc gcttgccgag gctcgtcgtt
     1801 cgcccgaaag gccgccgcga ggcggccttt cagcttaaag aggtgccggt gttacggttc
     1861 ggttcaaccg taactccggc acttcggttg aaccgaaacc gtaacaccgg caccgaatgg
     1921 gaacgccaat cgacttgatc gtgggcctcg gcaatccggg gccggaatat gcggccaccc
     1981 gccacaacgc ggggttctgg ttcgtcgatc tattggcgaa ggagcacggc ggatcgtttt
     2041 cgcgcgagcg caagctgcaa ggcgaagcgg ccgagatcgc gatcgcgggt aagcgcattc
     2101 gcttgttgaa gccgacgacg tacatgaact tgagcggcca gtcggtcggc gcggccgtca
     2161 actactacaa gattccgacc gagaacgtgc tcgtcgccta cgacgagctc gacttgccgg
     2221 cgggacgcat gcagctgcgc ttcgacggca gcagcgcggg ccacaacggc atcaacagcg
     2281 tcatcgagca catcggcagc aaattctggc gtctgcgctt cgggatcggc catccgcgcg
     2341 acggcgagca tgcgagcggc gagcgcaagg acgtcatcga ccacgtgctg aagcgcgccg
     2401 gcggcgatga ggaagacctg ctg
//
LOCUS       DASSNN010000621         3243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1703735, whole genome shotgun sequence.
ACCESSION   DASSNN010000621 DASSNN010000000
VERSION     DASSNN010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703735"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1423
                     /locus_tag="VFL84_11615"
     CDS             <1..1423
                     /locus_tag="VFL84_11615"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7609317.1"
                     /translation="QAFDAKSGALLWEYKRQYPEGVRGGTNRNLAIWGTTLIDAGADN
                     SMYAIDARTGQLVWETKVLEPTLPARASSGPIIANGKIITGRQCQPQATHESCVITAH
                     DAKTGKELWRTRTIPRKGEPGDETWGDVPMEQRWHVGTWMVPSYDPELDRIYIGTSVT
                     IPAPKFILGGSDKQHLYHNSTLALDPDNGKVVWYYQHLNDNWDLDHPFERLLVDTAVA
                     PNASEVAWINPRVRPGERRRVITGVPGKTGIVYTLDRATGEFLWARPTVFQNVVKNID
                     GATGKVENDPDRVPSASGQSFMVCPGTNGGKNWPAGAYSPRTNTMYMPMQNMCMNATV
                     GAGERDPSKVYGFDSEYIAAPGADKVGVVWAISAENGTTVFKHEQRVGVMSLVATGGG
                     LVFGGDVAGNFHALDDKTGEVLWETNIGAPVSGYPVSFAVDGKQYVAVTTGQSLVAGA
                     ARRVTPELTGQSPPAQVVVFALP"
     gene            1432..2091
                     /locus_tag="VFL84_11620"
     CDS             1432..2091
                     /locus_tag="VFL84_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609318.1"
                     /translation="MRLRASSSAAALCLGILLPAAQARADVVVTVQAPVRARVFFESD
                     RRPPEMPASAAEGSAACSNVFEIEAKIESSVDVVSPTAVHVYPESFEIVTRLKSTIYL
                     PDRAPDQLHEHEEGHRAIGEYFYRNAEVAAREAAQSLIGRSFEGIGLDRAEAEQRAGN
                     VVLAVLKDEYMKRMHARSAAANARYDEITKHGLNGRMPAKGAVELALAEARRQEALTA
                     Q"
     gene            2105..3064
                     /locus_tag="VFL84_11625"
     CDS             2105..3064
                     /locus_tag="VFL84_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7609319.1"
                     /translation="MRASRYVLTVAASFTVTVAAQAPQPEVERHVAAARAAAGEHAAM
                     VDRLCPRSGVERPVQGNRTAARGVPARESWHAEPVKVFDNLYFVGMTEFASWAVTTSE
                     GIIVIDPLFDYSVEDEVVGGLTKLGLDPKSIKYVLVSHGHLDHVGGAKLLQERFGARV
                     LLAAADWDLLDRDDPPWKPRRDLNVTDGMKLTLGDTTLTIYHTPGHTNGTVSTLIPLR
                     DGNARHLAALWGGTLFNFGPDAARFRAYAESAAKFREIATAAGADVLLSNHTDYDGSK
                     QKLPALAARRAGAPHPYVVGNDSVRRYLTVANECAQAALAGVQ"
BASE COUNT          564 a         1064 c         1121 g          494 t
ORIGIN      
        1 gcaggcgttc gacgcgaaga gcggcgcgct gctctgggag tacaagcgcc agtatccgga
       61 aggcgtgcgc ggcggcacga accgcaatct cgctatttgg ggcacgaccc tgatcgacgc
      121 gggggccgac aactcgatgt acgcgatcga cgcgcgcacg ggccagctcg tttgggaaac
      181 gaaggtcctg gagccgactc tgccggcgcg cgcgagctcg ggcccgatca tcgcgaacgg
      241 caagatcatc acgggccgcc aatgccagcc gcaggccacg cacgagtcgt gcgtgatcac
      301 ggcgcacgac gcgaagacgg gcaaagagct ctggcgcacg cggacgatcc cgcgcaaggg
      361 cgagcccggc gacgagacgt ggggcgacgt gccgatggag cagcgctggc acgtcggcac
      421 gtggatggtg ccgagctacg accccgagct cgaccgcatc tacatcggca cgtcggtcac
      481 gatcccggcg ccgaaattca ttctcggcgg ctcggacaag cagcacctct atcacaactc
      541 gacgctcgcg ctcgatccgg acaacggcaa ggtcgtctgg tactaccagc atctcaacga
      601 caactgggat ctcgaccatc cgttcgagcg gctgctcgtc gatacggccg tcgcgccgaa
      661 tgcgagcgag gtcgcgtgga tcaatccgcg cgtgcggccc ggtgaacggc gccgcgtcat
      721 cacaggtgtt ccgggcaaga ccggcatcgt ctacacgctc gaccgcgcaa ccggcgaatt
      781 cttgtgggcg cgtccgaccg tgttccagaa cgtcgtcaag aacatcgacg gcgcgaccgg
      841 caaggtcgag aacgaccccg accgcgtgcc gtcggcctcc ggccagtcgt tcatggtgtg
      901 ccccggcacg aacggcggca agaattggcc cgccggtgcc tatagcccgc gcacgaacac
      961 gatgtacatg ccgatgcaga acatgtgcat gaacgcgacc gtgggcgccg gcgagcgcga
     1021 cccgagcaag gtctacggct tcgactcgga atacatcgcg gcgccgggcg cggacaaagt
     1081 cggcgtcgtc tgggcgatct cggccgagaa cggcacgacg gtgttcaagc acgagcagcg
     1141 cgtcggcgtt atgtcgctcg tggcgacggg cggcggtctc gtcttcggcg gcgatgtcgc
     1201 cggcaacttc cacgcgctcg acgacaagac cggcgaagtg ctctgggaga cgaacatcgg
     1261 cgcgccggtc agcggctatc ccgtatcgtt cgccgtcgac ggcaagcagt acgtggccgt
     1321 gacgacgggc cagtcgctcg tcgccggcgc ggcgcgccgc gtgacgccgg agctgacggg
     1381 tcagtcgccc ccggcgcaag tcgtggtatt cgcgttgcca tagcgagatc catgcgcctg
     1441 cgcgcgtcct cgtcagcagc agcgttgtgc ctcggcatcc tgctgccggc ggcgcaggcg
     1501 cgggcggacg tcgtcgtcac ggtgcaggcg ccggttcgcg cgcgcgtgtt cttcgaaagc
     1561 gaccggcggc cgccggagat gccggcgagc gcggccgaag gctcggcggc gtgcagcaac
     1621 gtgttcgaga tcgaggcgaa gatcgaatcg tcggtagacg tggtctcgcc gacagcggtg
     1681 cacgtctatc ccgagagctt cgagatcgtc acgcgattga agagcacgat ctatttgccg
     1741 gatcgtgcgc cggaccagct gcacgagcac gaggaagggc accgcgcgat cggcgagtat
     1801 ttctatcgca acgcagaagt ggccgcgcgt gaggccgctc agtcgctgat cggccgttcc
     1861 ttcgagggca tcgggctcga ccgcgcggaa gcggagcagc gagccggcaa cgttgtcctc
     1921 gcggttctca aggacgaata catgaagagg atgcacgcac gcagcgcggc cgctaacgcg
     1981 cgctacgacg agatcacgaa gcacggcctg aacggccgga tgcccgcgaa aggcgcagtc
     2041 gagctcgcgc tcgcggaagc tcgcaggcaa gaagcactga cggcgcaatg acgaggagaa
     2101 cacgatgcgc gcttctcgat atgtactgac ggtcgcagcg tccttcaccg tgacggttgc
     2161 ggcgcaagcc ccgcagcccg aagtcgagcg tcacgtcgcg gccgcgcgcg ccgccgccgg
     2221 cgagcacgcg gcgatggtcg ataggctttg ccctcgctct ggagtcgagc ggcccgttca
     2281 aggcaatcgc accgccgcgc ggggcgtgcc ggcgcgcgaa tcatggcacg ccgagcccgt
     2341 gaaggtcttc gacaatctct acttcgtcgg tatgacggaa ttcgcgtcgt gggccgtcac
     2401 gaccagcgaa ggcatcatcg tcatcgatcc gctgttcgac tactccgtcg aggacgaggt
     2461 cgtcggagga ttgaccaagc tcgggctcga tcctaagagc atcaagtacg tgctcgtgag
     2521 ccacggtcac ctcgatcacg tcggcggagc gaagctgctg caggagcggt tcggcgcgcg
     2581 cgtgctactc gcggccgcgg actgggactt gctcgatcgc gacgatccgc cgtggaagcc
     2641 gcgccgcgac ttgaatgtaa cggacggcat gaagctcacg ctcggcgaca cgacgttgac
     2701 gatctatcac acgccggggc acacgaacgg aacggtatcg acgctgatcc cgctgcgcga
     2761 cggcaacgcg cgccatctcg ccgcgctctg gggcggcacg ctgttcaatt tcgggcccga
     2821 tgccgcgcgg ttccgcgcgt acgcggaatc ggccgccaag ttccgcgaga tcgcgacggc
     2881 ggccggcgcc gacgtgctgc tctcgaatca cacggactac gacggctcga agcagaagct
     2941 gcccgcgctc gccgcgcggc gtgccggtgc gccgcatccc tacgtcgtcg gcaacgactc
     3001 ggtgcgccgc tatctaaccg tggcgaacga gtgcgcgcag gccgcgctcg cgggcgtgca
     3061 gtagtcgttg gctaccggac gccggtacct tcgccgtagc ccggtggctt gagggccggg
     3121 atggaggtca tcgggctcgt cctgttcatg ctggcgaccg tggtgttgag cggcgtgctc
     3181 gtgcgcatcc tgccgctcgc ggtgcccacg ccgttgctgc agatcgcctt tggcgcagtg
     3241 ctt
//
LOCUS       DASSNN010000622         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1489582, whole genome shotgun sequence.
ACCESSION   DASSNN010000622 DASSNN010000000
VERSION     DASSNN010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489582"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2247)
                     /gene="mbhE"
                     /locus_tag="VFL84_11630"
     CDS             complement(<1..2247)
                     /gene="mbhE"
                     /locus_tag="VFL84_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HET7609320.1"
                     /translation="MLLALIVLLPFVGSICAAFMPANARNAEAWLAGAVATASAILVA
                     VQYGDVVAGGVVRTKLAWLPQYGLDFYLRMDGFAWLFALLVAFIGTLVVLYARYYMSA
                     QDPVPRFFAFLLAFMGAMLGTVLSGNLIQLVVFWELTGLTSFMLIAYWYHRADARRGA
                     RMAAVVTVGGGLALLLGVLALGEIVGSYDLEAVLAAGDTIRGHSWYPALLALILLGVL
                     TKSAQFPFHFWLPHAMAAPTPVSAYLHSATMVKAGVFLLARLWPALAGTDLWVLLVCG
                     AGAASLLGGTFTATYQRDMKGVLAYSTIGHLGLITFLLGMSTPLALVAAVFHMLNHAT
                     FKASLFMATGVVDHETGTRDLGRLNGLRHAMPITATLAAVAAAAMAGVPLLNGFLSKE
                     MFFEESIVVGGSVGMQILLPAIATLGGLFSVAYSLRFIHEVFFGAPAPDLPRKPRDPR
                     LMLVPSALLVITCVLVGVLPGQTIGPLLATAGMAILGDVPQYELAVWHGFTPALLMSL
                     VALAGGVGFYALLYWRGQTLTETPLLSRFDSKRMFDIANVALTRTAARAARWLFSRRL
                     QPQLVLIVASACAAVALPLSTGGWLEHDLAVAPVDPVFAALWIVGGACAIGAAWQAKF
                     HRLAALIMVGGAGLVVCLTFAWLSAPDLALTQIAVEVVTMVLFLLGLRWLPRRLEISD
                     ARRRTARARARRARDAVLAGVAGIGLGTLAFAVVTLPPAGLLAPFFFANALDPAGGHN
                     VVNLILVDF"
BASE COUNT          451 a          801 c          818 g          259 t
ORIGIN      
        1 gaagtcgacg aggatcaagt tcacgacgtt gtggccgccc gcgggatcca gcgcgttcgc
       61 gaagaagaac ggggcgagca agccggccgg cggcagagtg acgaccgcga atgcgagcgt
      121 gccgaggccg atgccggcaa cgcccgcgag cacggcgtcg cgcgcgcgcc gcgcacgcgc
      181 gcgagcggtg cgccgccgcg catcgctgat ctcgaggcgc cgcggcaacc agcgcagccc
      241 gagcaggaac agcaccatcg tcacgacttc gactgcgatc tgcgtcaatg cgaggtccgg
      301 cgccgacagc catgcgaacg tcaggcagac gacgaggccg gcgccgccga ccatgatcaa
      361 tgccgcgagc cggtggaact tggcctgcca cgcggcaccg atggcgcacg cgccgccgac
      421 gatccacaac gccgcgaaca cgggatcgac cggcgctacc gcgagatcgt gctcgagcca
      481 gccgcccgtc gacaacggca acgcgacggc ggcgcacgcg ctcgcgacga tcaacacgag
      541 ctgcggttgc aagcgccgcg agaacagcca acgcgcggcg cgggccgccg tgcgcgtcag
      601 cgcgacgttg gcgatgtcga acatccgctt gctgtcgaac cgcgacagca gcggcgtctc
      661 ggtcagcgtt tgcccccgcc agtagagcag cgcgtagaac ccgactccgc cggcgagcgc
      721 gaccaggctc atcagaagcg cgggagtgaa gccgtgccaa acggcgagct cgtattgcgg
      781 cacgtcgccc aagatcgcca tgcccgcggt cgcgagcagg ggcccgatcg tctgcccggg
      841 tagaacgccg acgagcacgc aggtgatgac cagcagcgcg ctcggcacga gcatcaggcg
      901 cggatcgcgg ggcttgcgcg gcaaatccgg cgcgggcgcg ccgaagaaca cctcgtggat
      961 gaagcgcaac gagtacgcga cgctgaagag cccgccgagc gtggcgatgg ccggcagcag
     1021 gatctgcatg ccgacgctgc cgccgacgac gatcgattcc tcgaagaaca tctccttcga
     1081 cagaaagccg ttcaagagcg gcacgccggc catcgcggcg gcggcgacgg ctgcgagcgt
     1141 cgccgtgatg ggcatcgcgt gccgcaggcc gttgagcctt cccaagtccc gcgtgccggt
     1201 ctcgtggtcg acgacgccgg tcgccatgaa cagcgacgcc ttgaaagtcg cgtggttcag
     1261 catgtggaac accgcggcga ccagcgcgag cggcgtgctc atgccgagca agaacgtgat
     1321 gaggcccaag tgcccgatcg tcgagtacgc gagcacgccc ttcatgtcgc gctgatacgt
     1381 ggccgtgaac gtgccgccga gcagcgacgc ggcgccggcg ccgcagacga gcaggaccca
     1441 caagtccgtg cccgcgagcg cgggccacag ccgcgcgagc aggaacacgc cggccttgac
     1501 catcgtggcg gaatgcaagt aggcggacac cggcgtaggc gccgccatcg cgtgcgggag
     1561 ccagaagtgg aacggaaact gcgcgctctt ggtcaggacg ccgagcagaa tcagcgcgag
     1621 cagcgccgga taccagctgt ggccgcggat cgtgtcgccg gccgcgagca cggcctcgag
     1681 atcgtagctg ccgacgatct cgcccaaggc gagcacgccg agcagcagcg cgagcccgcc
     1741 gccgacggtc accacggccg ccatgcgcgc gccgcgccgc gcgtcggcgc ggtgatacca
     1801 gtacgcgatc agcatgaacg acgtgaggcc cgtgagctcc cagaacacga cgagctggat
     1861 caagttgccc gacagcaccg tgccgagcat cgcgcccatg aacgcgagca ggaacgcgaa
     1921 gaagcgcggc accggatcct gcgccgacat gtaatagcgc gcgtacagca cgacgagcgt
     1981 gccgatgaac gcgacgagca gcgcgaacag ccacgcgaac ccgtccatgc gcagatagaa
     2041 gtcgaggccg tattgcggga gccaggcgag cttcgtgcgg acgacgccgc cggcgacgac
     2101 gtcgccgtac tgcaccgcga cgaggatcgc gctggccgtc gcgacggcgc ccgcgagcca
     2161 cgcctcggcg ttgcgcgcgt tggccggcat gaaggcggca caaatgctgc cgacgaaggg
     2221 cagaaggacg atcagcgcga ggagcacagt gcagcctcgc gcgaccccgt ctcgttgttc
     2281 gcgagcatcg gcatcgatcg attgaaccgc gcggtcttgc gaccacgac
//
LOCUS       DASSNN010000623         3741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1852961, whole genome shotgun sequence.
ACCESSION   DASSNN010000623 DASSNN010000000
VERSION     DASSNN010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852961"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VFL84_11635"
     CDS             complement(<1..873)
                     /locus_tag="VFL84_11635"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET7609321.1"
                     /translation="MSTSTSRTIRANIFFAEKDRALREDVHRLGELVGELVREQGGEA
                     LFDLVETARKLAIAHREGDQAAYGELKLLLGSLAPSTARDFIRAFSTYFQMVNMAEKV
                     HRIRRRRAYLRDASAPQPFGFVDILQRLKAQGVDGDEIERALAQVHIQPVFMTHPTEV
                     TRRTLLRKEQNIARHLVEMMDPYLTPHELEATLGQIRQEMTTGWQTVEGLDEGIRLRD
                     EAEHVLFFLTDVLYRMVPPLYESLESALAEAFPERGGGVRVPIVVQFGTWVGGDMDGN
                     PHVTAKSIRQTLARQ"
     gene            967..2484
                     /locus_tag="VFL84_11640"
     CDS             967..2484
                     /locus_tag="VFL84_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609322.1"
                     /translation="MIERLAIRFAAAAAAAWLAAGAGAGEWWGPGDGRPLPEQASYAN
                     AAGAIGILNTAGTIDTAANPFFLPLGTNGRACVTCHQPADGMALSVATVRERWDETQG
                     RDPLFAAIDGRNCPNLPAADPAAHSLLLTRGLIRVALPWPPRDANGVPLPVEITLEVV
                     RDPTGCNTSPVHGLTSPQPTVSVYRRPRPVANTKYLTHHNFGVGPFIAKSGLPAVRDP
                     DTGLPTSMNMMADARATTLKLQAADAARTHLELVRPLAPDTMRELVAFEQQIYVAQAR
                     DREAGDLTRGAPGFGPRNMAEEPAGVLGNNTTRWVFPIDDAWREPRTGTAAEREARAA
                     IARGHDVFMFRTFFIRDSMHLNSVGLGNPVKRTCSTCHGMHMTGMDVANGWMDIGTTN
                     LPWAREAPLNPWTKDVPQLPLFKVTCNADVAPHPFLGSVIYTQDPGRALVSGKCNDVG
                     AIVMQQFRGLAARAPYFSNGSAATLRELVDFYDRRYSIGYSEQEKHDLVAFLEAL"
     gene            2481..3266
                     /locus_tag="VFL84_11645"
     CDS             2481..3266
                     /locus_tag="VFL84_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609323.1"
                     /translation="MRKVRAGCLLTALAAAPLAAAELAVEQRNFVACPIVRDTASVPC
                     WLAEYDGELYFLTLQTDVSAPVTPPWLGHRVLVEGKAARERPRVCGGIVLEPVVLSVL
                     PELDAACNTAVLPAEDRYNLTFEPPRPPGPSAGRLAFAGAAAAAPPAEPARAFALRYD
                     FDGLIVFRHAGELTAILEAAQARKARSVAITGHRGSVLLSNGTTLVEQPAIARRRAEQ
                     VAELLRGAGLTGVEYRVRWHDEAGAADGNDDAALRRVTVELDP"
     gene            3285..>3741
                     /locus_tag="VFL84_11650"
     CDS             3285..>3741
                     /locus_tag="VFL84_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018871184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET7609324.1"
                     /translation="MGFAPSAIELTSTVFAPMARIPKQYTGEGADISPPLAWRNAPAG
                     TKAFALFVHDPDAPLVTPQAYGFTHWVLYNIPGSVSELREAEKSYTSGANSFGKPGYG
                     GPMPPELHGVHHYYFWLLALRADKKLPPGLSMQDLFAQIEPQVLGMNRLI"
BASE COUNT          575 a         1320 c         1265 g          581 t
ORIGIN      
        1 ttgcctcgcg agcgtctgac ggatcgactt cgccgtgacg tgcgggttgc cgtccatgtc
       61 gccgccgacc cacgtgccga actggacgac gatcggcacg cgcacgccgc cgccgcgctc
      121 gggaaatgcc tcggccaacg cgctctcgag gctctcgtag agcggcggca ccatgcgata
      181 cagcacgtcg gtcaagaaga acagcacgtg ctccgcctcg tcgcgcaggc gtatgccttc
      241 gtccaggcct tcgaccgtct gccagcccgt cgtcatctct tgccgaatct gaccgagcgt
      301 cgcttcgagc tcgtgcggcg tgagataggg atccatcatc tcgacgagat gccgcgcgat
      361 gttttgctcc ttgcgcagca gcgtccggcg cgtcacctcg gtcgggtgcg tcatgaacac
      421 gggctggatg tggacctgcg ccagcgcgcg ctcgatctcg tcgccgtcga cgccttgcgc
      481 tttgagccgc tgcaggatgt cgacgaagcc gaacggctgc ggcgcgctcg cgtcgcgcaa
      541 gtaagcgcgg cggcggcgga tccggtgcac cttctccgcc atgttgacca tctggaagta
      601 ggtcgagaac gcacggatga agtcgcgcgc cgtgctcggc gccagcgatc ccagcagcag
      661 cttgagctcg ccgtaggccg cttgatcgcc ttcgcgatgc gcgatcgcga gcttgcgcgc
      721 cgtttcgacg agatcgaaca gcgcctcgcc gccctgctcg cgcacgagct cgccgacgag
      781 ctcgccgagc cggtgcacgt cctcgcgcag cgcgcggtcc ttctccgcga aaaagatgtt
      841 ggcgcggatc gtccgggatg tcgaagtcga catggcgagc cagtttaccg cttgtcacgg
      901 gcacgagccg ggttgcatag tcgcgcgacg tacggcgcca cggcggcgcg cgaagggagc
      961 gtgggcatga tcgagcggct cgcaattcga ttcgcggcgg cggccgcggc cgcgtggctc
     1021 gcggcgggcg ccggcgccgg cgagtggtgg gggcccggcg acggacggcc gctgcccgag
     1081 caggcgtcgt atgcgaatgc cgccggcgcc atcggcattc tgaacacggc cgggacgatc
     1141 gacaccgctg ccaacccatt cttcctgccg ctcggcacga atggccgcgc ctgcgtgacg
     1201 tgccatcagc ccgccgacgg catggcgctg tccgtcgcga ccgtccgcga gcgctgggac
     1261 gagacccagg gacgcgatcc tctgttcgcc gcaatcgacg gccgcaactg tccgaatctg
     1321 ccggcagcag atccggccgc gcactcgctg ctgttgacgc gcgggctgat ccgcgtggcg
     1381 ctgccttggc cgccgcgcga tgcgaatggc gtgccgctgc ccgtcgagat cacgctcgag
     1441 gtcgtccgcg acccgacggg gtgcaacacg agcccggtcc acggcctcac gtctccgcag
     1501 ccgacggtat ccgtgtaccg gcgtccgcgg cccgtcgcga acacgaaata cctgacgcac
     1561 cacaacttcg gcgtcggccc gttcatcgcg aagagcggcc tgcccgccgt acgcgatccc
     1621 gacactgggc tgccgacgag catgaacatg atggccgacg cgcgcgcgac gacgctgaag
     1681 ctgcaagccg cggacgcggc gcgcacgcat ctcgagctcg tgcgcccgct cgcgcccgac
     1741 acgatgcgcg agctcgtcgc gttcgagcag caaatctacg tggcgcaggc gcgcgatcgc
     1801 gaagccggcg acctgacgcg cggtgcgccg ggctttggcc cgcggaacat ggctgaggag
     1861 cccgccggcg tgctcggcaa caatacgacg cgttgggtct ttccgatcga cgacgcatgg
     1921 cgcgagccgc gaacgggcac cgccgccgag cgcgaagcgc gtgctgcgat tgcgcgcggc
     1981 catgacgtgt tcatgtttcg gacttttttc atccgcgact ccatgcactt gaacagcgtc
     2041 ggcctcggca atcccgtcaa gcgcacgtgc tcgacctgcc atggcatgca catgacgggc
     2101 atggacgtcg cgaacggctg gatggacatc ggcacgacga atctgccctg ggcgcgcgaa
     2161 gcgccgctga acccgtggac gaaggacgtc ccgcagctgc cgctgttcaa ggtcacgtgc
     2221 aatgccgacg tcgcgccgca tccgtttttg ggatccgtca tctacacgca ggatccggga
     2281 cgcgcgctcg tctccggcaa gtgcaacgat gtcggcgcga tcgtcatgca gcaattccgc
     2341 ggcctcgcgg cgcgcgcgcc gtatttctcg aacggctcgg ccgcgacgct gcgcgagctc
     2401 gtcgacttct acgatcggcg ctattcgatc ggctactccg agcaggagaa gcacgatctc
     2461 gtcgcgttct tggaggcgct atgagaaaag tccgcgctgg ttgcctgctg acggcgctcg
     2521 ccgcggcacc gctcgcggcg gcggaattgg ccgtagagca acgcaacttc gtcgcttgcc
     2581 cgatcgtccg cgacaccgcg tcggtgccgt gctggctcgc cgagtacgac ggcgagctct
     2641 acttcttgac gctgcagacc gacgtcagcg cgcccgtcac gccgccgtgg ctcgggcatc
     2701 gcgtgctcgt cgagggcaaa gcggcgcgcg agcgcccgcg cgtctgcggc ggtatcgtgc
     2761 tcgagcccgt cgtgctgtcg gtgctgccgg agctcgatgc ggcgtgcaac acggccgtgc
     2821 tacccgccga ggaccgctac aacctgacgt tcgagccgcc gcggccgccg ggcccgagcg
     2881 ccggccggct tgcgttcgcg ggcgccgccg cggccgctcc gccggccgag ccggcgcgcg
     2941 ccttcgcgct gcggtacgat ttcgatgggc tcatcgtgtt tcgccacgca ggcgagctca
     3001 cggcgatcct cgaggcggcg caggcgcgga aagcgcgctc ggtcgcgatc acgggtcatc
     3061 gcggcagcgt gctgctgtcg aacggcacga ccctcgtcga gcaaccggcg atcgcgcggc
     3121 ggcgagccga gcaggtcgcg gagctgctgc gcggcgccgg cctcacgggc gtcgagtacc
     3181 gcgtgcgctg gcacgacgaa gccggagccg ccgacggcaa tgacgacgcc gcgctgcgcc
     3241 gcgtcacggt cgagctcgat ccctgaaatt cgaggagaac gaccatgggt ttcgcaccgt
     3301 ccgcaattga gctcacgagc acggtgttcg cgccgatggc gcgcattccg aaacaataca
     3361 cgggcgaagg cgccgacatt tcgccgccgc tcgcgtggcg caacgcgccg gccggcacga
     3421 aagcgttcgc gctgttcgtg cacgaccccg atgccccact ggtgacgccg caggcgtacg
     3481 gcttcacgca ctgggtcctc tacaacatcc cgggcagcgt cagcgagcta cgcgaagccg
     3541 agaagagcta cacgagcggc gcgaacagct ttggcaagcc cggctacggc ggcccgatgc
     3601 cgcccgagct gcacggcgtg caccactact acttctggct gctcgccttg cgcgccgaca
     3661 agaagcttcc gcccggcctc tcgatgcaag acctcttcgc ccaaatcgag ccgcaagtcc
     3721 tcggcatgaa ccggctgatc g
//
LOCUS       DASSNN010000624         4264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1118338, whole genome shotgun sequence.
ACCESSION   DASSNN010000624 DASSNN010000000
VERSION     DASSNN010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118338"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFL84_11655"
     CDS             <1..330
                     /locus_tag="VFL84_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HET7609325.1"
                     /translation="EDATGADIESAIWQQLRTCYDPEIPINIVELGLVYECKIERAPS
                     GNRIAKVKMTLTAPGCGMGEILAQDVREKIEVIPTVERADVELVFDPPWNQSMMSEEA
                     RLEAGLL"
     gene            350..868
                     /locus_tag="VFL84_11660"
     CDS             350..868
                     /locus_tag="VFL84_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7609326.1"
                     /translation="MKRLLLLRHAKAVPATEPLVDADRPLAERGERDARRIGERLREQ
                     RLRPALIVASPAARALRTAQLVAGALDHPSDAIALDRRLYLAEPTAIVEIIGAQDAAI
                     ETLLVVGHNPGLTELVHDLLPTFEVDDLPTCAVVGLDYAEAESWDRIAGAAAMLGYYD
                     FPKNSRAPISAR"
     gene            complement(855..1280)
                     /locus_tag="VFL84_11665"
     CDS             complement(855..1280)
                     /locus_tag="VFL84_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET7609327.1"
                     /translation="MTRAEESDDDADRLRIDRWLWCARFFKSRSAAAEAVRGGHVRLN
                     GQRVKPAHAVKIGDALQLTLAYGVERDVSVALIPARRGPAPEAAASYVESPESIERRR
                     KAAEERAARAAYVPPTAGRPDKRTRRLLLRARQRGLSER"
     gene            complement(1277..3142)
                     /gene="sppA"
                     /locus_tag="VFL84_11670"
     CDS             complement(1277..3142)
                     /gene="sppA"
                     /locus_tag="VFL84_11670"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET7609328.1"
                     /translation="MDKDNRALPLRIVAAVWHGLDRLRRVLHLILLLGLFMVLIAGAV
                     GERVFIPQTAALVIAPRGVLVDQLSGDALDRALARAQGAPLAETLVRDVVDALRAARD
                     DERIKAVVLDLDELTGAGLSKLQDIAVELRRFKESGKPVIAVGDSYTRDQYYLAAQAD
                     DVYMHPMGLVLVDGYSRFLPYYKSLLDKIYVDYNVWTVGEYKSFVEPITRDDMSPQDE
                     EASKMYLDELWGAYQADVTSARGMAAPALQRYADDIVALLDEANGNTAQLAKDYGLVD
                     DLLTRDRMRERIREKMGEPKSSSGQPDAYTQVSLDDYVTWLRTEAPPESRPSKVAVIV
                     AAGEILDGRQAPGTIGGDSTSELIRRARADDSVKALVLRVDSGGGSAFASDLILRELE
                     RFQETERPVVVSMGSVAASGGYWISMGADEIWASPTTLTGSIGVGATIPTVPRTLDAL
                     GVHVDGVGTTELAGGLEITRPIGESMKGLIGLSVRHTYDEFVGKVAEHRERSVEEVEA
                     AAQGRVWTGSDALSRGLVDKLGGLPEAIESAAELAGLEEDTYMLDYVEQQLGFVETLM
                     LSMTAKALAGVDRLVDLPRWPATVTQALSTLEPLAFIDRLNDPRGIYAYCFCDTQ"
     gene            complement(3164..>4264)
                     /locus_tag="VFL84_11675"
     CDS             complement(3164..>4264)
                     /locus_tag="VFL84_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HET7609329.1"
                     /translation="VAIPLFTFAGFLLSAGQAPARLVRLSQALLGWLPGGLAIISLIA
                     CAIFTAFTGASGITIIALGALLYPALKQVGYDERFNLGLITSSGSLGLLFAPSLPLIL
                     YGIVAEAPIDDLFVAGLLPGILLIVVLGAYCYWRNRRLDYSVTERSWSAAWDAIWAAR
                     WDLPLPIVVLGGIYGGFFAVSEAAAVTALYVVLTELVILREVAWRDVPRIMRESMVLV
                     GAILAILGVSLASTNLMIDTGVPQRLFALVSDYVTGPTTFMIVLLLFLLILGAILDIF
                     SATVLIVPLLLPIAAQYGIDPIHLGIVFLAAMELGYLTPPVGLNLFIASHRFEKDITE
                     IYWATQPFLIVSLLAVIIIAFWPSLSLMLLNR"
BASE COUNT          748 a         1457 c         1420 g          639 t
ORIGIN      
        1 gaggacgcga ccggggccga catagaatcg gccatatggc agcagctgcg cacctgctac
       61 gacccggaga taccgatcaa catcgtcgag ctcgggctcg tctacgaatg caagatcgag
      121 cgcgcgccgt ccgggaatcg catcgcgaag gtcaagatga cgttgacggc gcccggctgc
      181 ggcatgggcg agatcctcgc gcaggacgtg cgcgagaaga tcgaagtgat accgacggtc
      241 gagcgcgcgg acgtcgagct cgtgttcgac ccgccgtgga accagtcgat gatgtcggag
      301 gaggcgcggc tcgaagccgg cctcttgtga cccggcggcg cctctagaga tgaaacggct
      361 cttgttgctg cgtcacgcga aggccgtgcc ggccacggag cctttggtcg acgccgatcg
      421 cccgctcgcc gagcgtggcg agcgcgatgc gcgccggatc ggcgaacggc tgcgcgagca
      481 gcggctgcgc ccggcgctga tcgtggcgag cccggcggca cgcgcgctgc gcaccgcaca
      541 gctcgtggcc ggcgccctcg atcatcccag cgacgcgatc gcgctcgatc gccggctcta
      601 tctcgccgag cccaccgcga tcgtcgagat catcggcgcc caggatgccg cgatcgaaac
      661 gctgctcgtc gtcggtcaca acccgggcct gaccgagctc gtgcacgatc tattgcccac
      721 cttcgaggtc gacgacttgc cgacatgcgc cgtcgtcggt ctcgactacg ccgaggcgga
      781 aagttgggac cgcattgcgg gcgcggccgc gatgctcggc tattacgact tcccgaagaa
      841 ctcgcgcgcg ccgatcagcg ctcgctgagc ccgcgctgcc gggcacgcag cagcagacgg
      901 cgcgtgcgct tgtcgggcct gccggccgtc ggaggaacgt acgcggcgcg cgccgctcgc
      961 tcctcggcgg ctttgcggcg ccgctcgatc gactcggggc tctccacgta gcttgcggcg
     1021 gcttcgggcg ccgggccgcg ccgcgccggg atcagggcta cgctcacgtc gcgctcgaca
     1081 ccgtaggcca acgtgagctg cagcgcgtcg ccgatcttga cggcgtgcgc cggcttcacg
     1141 cgttgcccgt tgagacgcac gtggccgccg cgcacggctt cggccgccgc gctgcggctc
     1201 ttgaagaatc gcgcgcacca cagccagcgg tcgatgcgca gccggtccgc gtcgtcgtcg
     1261 ctctcttccg cccgtgtcac tgcgtgtcgc agaagcagta cgcgtagatc ccgcgcgggt
     1321 cgttgagacg gtcgatgaag gcgagcggct ccaaagtcga tagagcctgc gtcaccgtgg
     1381 cgggccagcg cggcaggtcg acgagccgat cgacgccggc gagcgctttg gccgtcatcg
     1441 acaacatcag cgtctccacg aagccgagct gttgctcgac gtaatcgagc atgtaagtgt
     1501 cctcctcgag ccccgcgagc tccgcggccg actcgatggc ctccggcaag ccgccgagct
     1561 tgtcgacgag cccgcgagag agcgcatcgc tgccggtcca gacgcggccc tgtgccgccg
     1621 cctcgacctc ctcgaccgag cgctcgcggt gctcggcgac cttgccgacg aactcgtcgt
     1681 aggtgtgccg aaccgaaagg ccgatcagac ctttcatgct ctcgccgatg ggccgcgtga
     1741 tctcgagccc gcccgcgagc tccgtcgtgc cgacaccgtc gacgtgcacg cccaatgcgt
     1801 cgagcgtgcg gggaaccgtc gggatcgtcg cgccgacgcc gatcgacccg gtcaaggtcg
     1861 tcgggctcgc ccaaatctcg tccgcgccca tcgagatcca gtagccgccg gacgccgcga
     1921 cgcttcccat ggaaacgacg acgggccgct ccgtctcctg aaagcgctcg agctcgcgca
     1981 gaatcagatc cgacgcgaac gcgctgccgc cgccggagtc gacgcgcaac accagcgcct
     2041 tgaccgagtc gtcggcccgc gcgcgccgaa tcagctcgga cgtcgagtcg ccgccgatcg
     2101 tgcccggcgc ctgcctgccg tcgaggatct cccccgccgc aacgatgacg gcgaccttgc
     2161 tcgggcgcga ctcgggcggc gcttcggttc gcagccacgt gacgtagtcg tcgagcgaaa
     2221 cttgcgtata cgcgtcgggc tgacccgagc tcgacttcgg ctctcccatt ttttcccgga
     2281 tccgctcgcg catcctgtcg cgcgtcagca ggtcgtcgac gagcccgtag tccttggcga
     2341 gctgcgcggt gttgccgttg gcttcgtcga gcagcgcgac gatgtcgtcg gcgtaccgct
     2401 gcagcgccgg cgcggccatg ccgcgcgccg aagtcacgtc ggcctggtac gcgccccaaa
     2461 gctcgtcgag gtacatcttg cttgcctcct cgtcctgagg cgacatgtcg tcgcgcgtga
     2521 tcggctcgac gaacgacttg tactcgccga cggtccagac gttgtagtcg acgtagatct
     2581 tgtcgagcag cgacttgtag tacggcaaga atcggctgta gccgtccacg aggaccaagc
     2641 ccatcggatg catgtagacg tcgtcggcct gcgcggcgag gtaatactga tcgcgcgtgt
     2701 agctgtcgcc gaccgcgatg acgggcttgc cgctctcctt gaagcggcgc agctcgaccg
     2761 cgatgtcctg cagcttgctc aatccggcgc ccgtcagctc gtcgagatcg agcacgacgg
     2821 ccttgatgcg ctcgtcgtcg cgcgcggcgc gcaacgcgtc gacgacgtcg cgcacgagcg
     2881 tttcggcgag cggcgctccc tgcgctcgcg cgagcgcgcg atcgagcgca tcccccgaca
     2941 gctgatcgac gagcacgccg cgaggcgcga tgacgagcgc agccgtttgc ggtatgaaga
     3001 cacgctcgcc gacggcgccg gcgatcagca ccatgaaaag cccaagcagc agaatcaggt
     3061 gcagcacgcg gcgcagccgg tcgaggccgt gccagacggc cgccacgatt ctgagcggca
     3121 acgctcgatt gtccttgtcc atatctcgtc cctacgaaga tgttcagcgg ttgagcagca
     3181 ttagcgacag actaggccag aacgcaatga ttatcacggc caacagcgac acgatcaaaa
     3241 atggctgggt cgcccaatag atttccgtga tgtccttctc gaagcgatga ctggcgatga
     3301 acagattcaa gcccacgggc ggggtcagat agccgagctc catcgcggcc aggaacacga
     3361 tgccgaggtg gatcggatcg atgccatact gcgcggcgat aggcagcagc agcggcacga
     3421 tcagcacggt ggccgaaaat atgtcgagga tcgcgccgag gatcagcagg aacaggagca
     3481 gcacgatcat gaacgtcgtc gggcctgtca cgtagtcgct gacgagggcg aacagccgct
     3541 gcggaacgcc cgtgtcgatc atgaggttcg tcgacgcgag cgatacgccg agaatcgcca
     3601 agatcgcgcc cacgagcacc atcgattcgc gcatgatgcg cggcacgtcg cgccacgcga
     3661 cctcgcgcag gatcacgagc tcggtcagca cgacatagag cgccgtcacg gccgccgctt
     3721 cggagacggc gaagaaacct ccgtagatgc cgcccaacac gacgatcggc agcggcaagt
     3781 cccagcgcgc cgcccagatg gcgtcccagg cggccgacca cgagcgctcc gtcaccgagt
     3841 aatcgaggcg ccgattgcgc cagtagcagt aggcgccgag cacgacgatc agcaggatgc
     3901 cgggtaagag acctgcgacg aacagatcgt cgatcggcgc ttcggcgacg atgccgtaca
     3961 ggatcaacgg cagcgacggc gcgaacagga gacccaggct gccggacgac gtgatgagcc
     4021 cgagattgaa gcgctcgtcg tagccgacct gcttgagcgc cgggtacagg agcgcgccga
     4081 gcgcgatgat cgtgatgccc gaagcccccg tgaacgccgt gaagatcgcg caagcgatga
     4141 gcgagatgat cgcgagcccg ccgggcagcc agccgagcag cgcctgcgac aggcgcacga
     4201 gcctcgccgg cgcttgcccc gcgctcaaca agaagccggc gaacgtgaac agcgggatcg
     4261 cgac
//
LOCUS       DASSNN010000625         4654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_846928, whole genome shotgun sequence.
ACCESSION   DASSNN010000625 DASSNN010000000
VERSION     DASSNN010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846928"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VFL84_11680"
     CDS             complement(<1..1274)
                     /locus_tag="VFL84_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET7609330.1"
                     /translation="MGAGSRLPLGATVQDGGVNFAVFCPRATRVWLRLYRDDADSSPI
                     AELELTAAAHRTYGFWHVFVEGARAGWFYTWRAEGPNEPAAGLRFDAQRELLDPWARL
                     VSDAAWNRAAAIKGDPRSAFRAEIVGPDDYDWGGDEPLKRSLEGAVIYELHVGGYTRH
                     PSSGVANPGTFLGLVEKIPHLKSLGITDVELLPVFAFDRQDVPAATAALGLHNFWGYS
                     PVGFFAPHSPYAASGDARREFRDMVKALHAEGIGVILDVVLNHTAEGGEDGPVISFKG
                     FGNEFFYHLDPKDKRKYRDFTGCGNTINCNHPLVARFLLQCLEFWVAEMHVDGFRVDL
                     ASVLSRGEDGEPMYHAPVLWSIEFSDVLARARLIAEAWDAGGLYQVGDFPGFRWMEWN
                     GKYRDTLRRYLRGDAGLIGDVATRLMGSSDFYA"
     gene            complement(1307..3934)
                     /gene="glgP"
                     /locus_tag="VFL84_11685"
     CDS             complement(1307..3934)
                     /gene="glgP"
                     /locus_tag="VFL84_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET7609331.1"
                     /translation="MSNSVFALQVSPSIPPAIRRLDDLAKNFWFSWNPALGQLFRKLD
                     PTLWRKVEASPRQFLRSVDQSILDHAATDPRFIDEYKQIVAAFDEYLGSKPAFSDGLE
                     PGDLIAYFCAEYGWHESFPIYSGGLGVLAGDHCKTASDLNLPFVAVGLLYRHGYFHQR
                     IDRSGQQMPDYPPIDPRSAPLSIATHADGSEVRVMSPALGRDVSVRVWKAPVGRVTVL
                     LLDTDVPENVDEDRAITGKLYGGSDELRQLQEAVLGIGGVRALRALGLKPTVWHINEG
                     HAAFMMLERLREYTTAGLPFAAALEATAASTVFTTHTPVSAGHDVFPPERVARQFASF
                     PAELGITAEQLLDLGRAPERPDVFNMTRLALKGASAVNAVSKIHGRVSSRLCASAWPD
                     VPPAENPMGFVTNGVHVATFLRHTWAKLFDKHLGPDWRARMMDRTLIARIEAIPDELF
                     WETRQKVKSQMFGVLRERLTLQYQRNGLSEAHVQRLLKVLDPEKPDVLTVGFARRFAV
                     YKRATLLVADLGWLEQLVRDEQRPVVFVFAGKAHPHDEPAQWMMREIQRISSLPPFVG
                     KILLLEGYDLGLGRLLTSGVDVWLNTPVAPQEASGTSGMKAAMNGTVNLSVLDGWWAE
                     AYDGRTGYRNGWGIPPARDEQGDADRDRQDSQTLYETLQDEVVPLYYARDPRLGYSPE
                     WVRICKRSMASVLPAFNSERVLRDYLHEFYGPAARQGRVLAADGFKGARELGAWKEKV
                     RAAWPGVTVKLLGGAAAEISFAERISLEVEVALNGLAPSDVRVECQVRRVLGSELVVP
                     VQGYAENRRPKHGLSYLEGRAVLLAPFTPGDVDEAGNCRYRLELQPPWAGRLEYEVRA
                     VPDHPHLSHPYELGLMRRL"
     gene            complement(4047..>4654)
                     /locus_tag="VFL84_11690"
     CDS             complement(4047..>4654)
                     /locus_tag="VFL84_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HET7609332.1"
                     /translation="ADFYEGPIEDEEWLAIKRNRVPGNAALSRRLIEALLSDIDVAYA
                     EEWKFDHGIMVPLHFLTPRYDLPVIPVNVNCQGPPLTPLKRAYEFGRALRRACDAQPE
                     RIALVGTGGISHWPATPDSGTINEAWDREFLKRFLANDAEALTSYTDEETLRDAGQGG
                     FEIRTFLAVAGATEGATGELLFYAPIPIFAVGCTVATMTVK"
BASE COUNT          745 a         1589 c         1590 g          730 t
ORIGIN      
        1 gcgtagaagt cgctgctgcc catgaggcgc gtcgcgacgt cgccgatgag tcccgcatcg
       61 ccgcgcaggt accggcgcag cgtgtcgcga tacttgccgt tccactccat ccagcggaag
      121 cccgggaagt cgccgacctg atagagtccc cccgcgtccc acgcctcggc gatgaggcgg
      181 gcgcgcgcga ggacgtccga gaactcgatg ctccagagca cgggggcgtg atacatgggc
      241 tcgccgtcct cgccgcgcga gagcacgctc gcgaggtcga cgcgaaagcc gtcgacgtgc
      301 atctcggcga cccagaactc gaggcattgc agcaggaagc gagcgacgag cggatggttg
      361 cagttgatcg tgttgccgca gcccgtgaag tcgcggtact tgcgcttgtc cttcgggtcg
      421 aggtgataga aaaactcgtt gccgaacccc ttgaacgaga tgacgggccc gtcctcgccg
      481 ccctcggccg tatggttcag cacgacgtcg aggatcacgc cgatgccctc ggcgtgcagc
      541 gctttgacca tgtcccgaaa ctcgcggcgc gcgtcgccgc ttgccgcgta cgggctgtgc
      601 ggcgcgaaga agccgacggg actgtagccc cagaagttgt gcagaccgag cgcggccgtc
      661 gccgccggta cgtcttgccg gtcgaacgcg aacacgggca agagctcgac gtcggtgatg
      721 ccgagcgact tcaggtgcgg aatcttctcg accagcccca agaacgtgcc gggattcgcg
      781 acgccggacg atggatggcg cgtgtatccg ccgacgtgca gctcgtagat gacggcgccc
      841 tcgagcgacc gcttcagcgg ctcgtcgccg ccccagtcgt agtcgtccgg cccaacgatc
      901 tcggcgcgga acgcggaccg gggatcgcct ttgatggcgg cggcgcggtt ccaggccgcg
      961 tcgctgacga gccgcgccca cgggtcgagc agctcgcgct gcgcgtcgaa gcgcaagccc
     1021 gccgcgggct cgttcggccc ctctgcgcgc caggtgtaga accagcccgc gcgcgcgcct
     1081 tcgacgaaca cgtgccagaa gccgtacgtc cggtgagccg cggccgtcag ctcgagctcc
     1141 gcgatcgggc tcgagtcggc gtcgtcgcga tagaggcgca gccagacccg cgtcgcgcgc
     1201 gggcagaaga ccgcgaagtt cacgccgccg tcctgcaccg tggcgccgag aggcagccgc
     1261 gagcccgcgc ccaggccgag cttcggcgga ttgtcgcggg cggcgctcaa agcctgcgca
     1321 tcaagccgag ctcgtacgga tgcgacaggt gcggatgatc gggcaccgcg cgcacttcgt
     1381 attcgagcct gcccgcccac ggcggctgga gctcgagccg gtagcggcaa ttgccggcct
     1441 cgtcgacgtc gccgggcgtg aacggcgcca gcagcacggc gcgcccttcg agatagctca
     1501 agccgtgctt cggccggcgg ttctcggcgt agccttgcac gggcacgacg agctccgagc
     1561 cgagcacgcg ccggacctga cattcgacgc ggacgtcgct cggcgccaag ccgttcagcg
     1621 cgacctcgac ctcgagcgaa atccgctcgg cgaacgagat ctccgcggcc gcgccgccga
     1681 gcagcttcac ggtcacgccg ggccacgcgg cgcgaacctt ttccttccag gccccgagct
     1741 cgcgcgcgcc cttgaagccg tcggccgcca acactctgcc ctggcgcgcc gcgggcccat
     1801 agaactcgtg cagatagtcg cggagcacgc gctcgctgtt gaacgccggc agcacgctcg
     1861 ccatcgaccg cttgcagatc cgtacccact ccggcgagta gccgagcctt ggatcgcgtg
     1921 cgtaatagag cggaacgacc tcgtcctgca gcgtttcgta cagcgtctgc gagtcctgcc
     1981 ggtcgcggtc ggcgtcgccc tgctcgtcgc gcgcgggcgg tatgccccag ccgttacggt
     2041 acccggtccg gccgtcgtag gcctcggccc accagccgtc gagcacgctc aagttcacgg
     2101 tgccgttcat cgcggctttc atgccggacg tgccgctcgc ttcctgcggc gcaacgggcg
     2161 tgttgagcca gacgtctacg cccgacgtca gcaagcggcc gagccccaag tcgtagccct
     2221 cgaggagcag gatcttgccg acgaagggcg gcaagctcga gatccgctgg atctcgcgca
     2281 tcatccattg cgcgggctcg tcgtgcggat gcgccttgcc ggcaaagacg aacacgacgg
     2341 ggcgctgctc gtcgcggacg agctgctcga gccagccgag gtcggccacg agcagcgtcg
     2401 cgcgcttgta gacggcgaaa cgccgcgcga agccgaccgt cagcacgtcg ggcttttccg
     2461 gatcgagcac cttcaggagg cgctgcacgt gcgcttcgct caagccgttg cgctggtatt
     2521 gcagcgtgag cctctcgcgg agcacgccga acatctggct cttgaccttt tgccgcgtct
     2581 cccagaacag ctcgtcgggg atcgcctcga tgcgcgcgat cagcgtgcga tccatcatgc
     2641 gggcgcgcca atcggggccc aagtgcttgt cgaacagctt ggcccaggta tggcgcagga
     2701 acgtcgcgac gtgcacgccg ttcgtgacga agcccatcgg attttcggcg ggcggcacgt
     2761 cgggccaagc gctcgcgcac aaccgcgacg acactcggcc gtgaatcttg ctgacggcgt
     2821 tgacggccga cgcgcctttc agcgcgagcc gcgtcatgtt gaacacgtcg ggacgctcgg
     2881 gcgcacggcc caagtcgagc agctgctcgg ccgtgatgcc gagctccgcg gggaagctcg
     2941 cgaactggcg cgccacgcgc tcgggaggaa acacgtcgtg gcccgcgctg accggcgtgt
     3001 gggtcgtgaa caccgtgctc gcggccgtcg cttcgagcgc ggcggcgaac ggcaggcccg
     3061 ccgtcgtgta ctcgcgtaag cgttcgagca tcatgaaggc ggcgtggccc tcgttgatgt
     3121 gccaaaccgt cggcttgagc ccgagcgcgc gcagcgcgcg cacgccgccg atgccgagca
     3181 cggcttcctg cagctggcgc agctcgtcgc tgccgccgta gagcttgccc gtgatggccc
     3241 gatcctcgtc gacgttctcg ggcacgtccg tgtcgagcag caagaccgtc acgcgcccga
     3301 ccggcgcctt ccagacgcgc acggagacgt cgcggccgag cgccggagac atgacgcgga
     3361 cctcgctgcc gtcggcatgc gttgcgatcg acagcggcgc gctgcgcgga tcgatcggcg
     3421 gatagtcggg catctgttgg ccgctgcggt cgatgcgctg gtgaaaatag ccgtgccgat
     3481 agagcagccc cacggcgacg aacggcaggt tcaagtcgct ggccgtcttg caatgatcgc
     3541 cggcgagcac gcccaagccg cccgagtaga tcggaaagct ctcgtgccag ccgtactcgg
     3601 cgcagaagta ggcgatcagg tctcccggct cgagcccgtc gctgaaggcc ggcttgctgc
     3661 ccaggtactc gtcgaacgcc gcgacgatct gcttgtattc gtcgatgaac cgcggatcgg
     3721 tcgcggcgtg atcgaggatg ctttgatcga cggagcgcag gaattgccgc gggctcgctt
     3781 cgactttgcg ccagagcgtg ggatcgagct tgcggaacag ctgtccgagc gccgggttcc
     3841 agctgaacca gaagttcttc gcgaggtcgt cgagccgacg gatcgcgggt gggatgctgg
     3901 gcgaaacctg tagcgcgaac acgctgttcg acatgacaaa tccttttggt ggcgaataga
     3961 acggccagac gggtatcccc tgaaacccgc cgccgtgccg tcttgagtca ggctatttta
     4021 ggggaagcgg cgcgtccacg ccgcccctac ttcacggtca tcgtcgccac ggtgcagccg
     4081 accgcgaaga tcgggatcgg ggcgtagaag agcagctcgc cggtcgcgcc ctcggtcgcg
     4141 cccgcgaccg cgagaaaggt ccggatctcg aagccgccct gccccgcgtc gcgcagcgtc
     4201 tcctcgtcgg tatacgacgt cagcgcttcc gcgtcgttcg cgaggaagcg cttcaagaac
     4261 tcgcggtccc aggcctcgtt gatcgttccg gagtcgggcg tcgcgggcca atgcgagatg
     4321 ccgcccgtgc cgacgagcgc gatgcgctcg ggctgcgcgt cgcaggcgcg gcgcagtgcg
     4381 cggccgaact cgtaggcgcg cttgagaggc gtcagcggcg ggccttggca gttcacgttg
     4441 acgggaatga cgggcaagtc gtagcgcggc gtcaggaaat gcagcggcac catgatgccg
     4501 tgatcgaact tccactcctc ggcataggcc acgtcgatgt cggacagcag cgcctcgatc
     4561 aagcgccgcg acaaagccgc attgcccggc acgcgattgc gcttgatcgc gagccattcc
     4621 tcgtcctcga tcggcccttc gtagaagtcg gcca
//
LOCUS       DASSNN010000626         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_483367, whole genome shotgun sequence.
ACCESSION   DASSNN010000626 DASSNN010000000
VERSION     DASSNN010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483367"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFL84_11695"
     CDS             <1..195
                     /locus_tag="VFL84_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609333.1"
                     /translation="LVLGVAGALALGRVLASQIPEVRAADPFVLAGAAFVLATAALFA
                     SWLPARRAARVDPLQALRQD"
     gene            complement(192..605)
                     /locus_tag="VFL84_11700"
     CDS             complement(192..605)
                     /locus_tag="VFL84_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET7609334.1"
                     /translation="MVLVDTNVVVDVWQRNAEWHPWSRAQLRAQSQVHELVINPIIYA
                     ELSLNYESIDTLDVGVADLKLRLLDLPRAALFLAGHAFRKYRRAGGGKSGVLPDFFIG
                     AHAAVLGCAILTRDAARYRAYFPTVELLTPAQLAS"
     gene            complement(606..845)
                     /locus_tag="VFL84_11705"
     CDS             complement(606..845)
                     /locus_tag="VFL84_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET7609335.1"
                     /translation="MATTITVKGQVTIPKHIRDALKLTPGSQVEFAVENEKVVLYKAG
                     APRSGAKKDRFDRALGAAEIKWDPDELMALLRGDD"
     gene            1049..>2408
                     /locus_tag="VFL84_11710"
     CDS             1049..>2408
                     /locus_tag="VFL84_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7609336.1"
                     /translation="MLPEQFHPAVAGWFRGAFPGPTSVQLKAWDAIGSRKHTLISAPT
                     GSGKTLAAFLGVIDLLAKQAADGTLRDETQILYVSPLKALSNDIEKNLRAPLEGIGRE
                     FAARMLGPAPIRAAVRTGDTPQSERARMRRQPPHILVTTPESLYILLTTESGRNMLRT
                     VKTVIVDEIHALAGNKRGAHLALSLERLEALCAEAPTRVGISATTKPIDVMTSFLTGV
                     TDEPCTVIDEGHARERDLTLELPGSPLEAIMAAEVWIELYDRLAEMIRQHRTTLVFVN
                     TRRHAERAARHLAERLGDDAVTSHHGSLAKEHRLKAEERLKAGQLKALVATASLELGI
                     DIGDVDLVCQLGSPHGISAFLQRVGRSGHGLGRVPKGRLFPLSRDDLVECTALLASIQ
                     RRELDAIVLRPQPLDVLAQQIVAEVGVQEWKVQDLHRRFTHAATYKDLPLEEFVGVAR
                     MVA"
BASE COUNT          374 a          840 c          806 g          388 t
ORIGIN      
        1 ctcgtgctcg gcgttgccgg cgcgctcgcg ctcggccgcg tgctcgcttc gcaaatcccc
       61 gaggtgcgtg ccgccgaccc gttcgtgctc gccggcgcgg catttgtcct ggcgactgct
      121 gctttgtttg cgagctggct cccggctcgc cgcgcggcgc gcgtcgaccc gctgcaggcg
      181 ctgcggcaag actagctcgc tagctgcgcc ggcgtgagca gctcgactgt cgggaagtac
      241 gcgcgatagc gcgccgcgtc gcgcgtcaag atcgcacagc cgagcaccgc ggcatgtgcg
      301 ccgatgaaga agtccgggag cacgccgctc ttccctccgc cggcgcgccg gtacttgcga
      361 aacgcatggc cggcgagaaa gagcgcggcg cgcggcaggt cgagcagcct gagcttgagg
      421 tcggcgacac ccacgtcgag ggtgtcgatg gactcgtaat tcagcgacag ctcggcgtag
      481 atgatcgggt tgatgacgag ctcgtgcact tgcgactggg cgcgcagctg cgcgcgggac
      541 caggggtgcc actcggcgtt acgttgccag acgtcgacga cgacgttggt gtcgacgagc
      601 accattcaat cgtcgccgcg taagagcgcc atgagctcgt cggggtccca cttgatctcg
      661 gcggccccga gcgcgcggtc gaagcggtcc ttcttcgcgc cactgcgcgg tgcgccggct
      721 ttgtacagca cgaccttctc gttctcgacg gcgaactcga cttggctgcc gggcgtgagc
      781 ttcagagcgt cgcgaatgtg tttcgggatc gtcacttgcc ctttgaccgt gatcgtcgta
      841 gccatggcgg gtcatccggt attacttgga gcccacacgg taataccgcc gaccgctcgt
      901 ggcaacgaac gaatcccggc tttgcgtgcc gaattcacgt caagagatgt ccggtttcgt
      961 cgctacgctg atcgactgcg cgcgccttgg gccgcgccag gctccccgac actggacata
     1021 aacacagctt ttcggtaaac ctaggcccat gctccccgaa cagttccacc ccgccgtcgc
     1081 cggctggttc cgcggcgctt tccctgggcc gacgagcgtc cagctcaaag cctgggacgc
     1141 gatcggctcg cgcaagcaca cgctgatcag cgcgccgacg ggctcgggca agacgctcgc
     1201 cgcgttcctc ggcgtcatcg acctgctcgc gaagcaggcc gccgacggca cgctcaggga
     1261 cgaaacgcag atcctctacg tctcgccgct gaaggcgctc tcgaacgaca tcgagaaaaa
     1321 cttgcgcgcg ccgctcgaag gcatcggccg cgagttcgcg gcccgcatgc ttgggccagc
     1381 gccgattcgc gcggccgtgc gcacgggcga tacgccgcag agcgagcgcg cgcgcatgcg
     1441 ccgccagccg ccgcacattc tcgtcacgac gccggagtcg ctgtacatcc tgctgacgac
     1501 cgagtcgggc cggaacatgc tgcgcacggt caagaccgtc atcgtcgacg agatccacgc
     1561 gctcgccggc aacaagcgcg gcgcgcatct cgcgctgtcg ctcgagcggc tcgaagcgct
     1621 ctgcgcagag gcgcccacgc gcgtcggcat ctcggcgacg acgaagccga tcgacgtcat
     1681 gacgagcttc ctcacgggcg tcacggatga gccctgcacg gtcatcgacg aaggccacgc
     1741 gcgcgagcgc gatctcacgc tcgagctgcc gggctcgccg ctcgaggcga tcatggcggc
     1801 cgaggtctgg atcgagctct acgatcgtct ggccgagatg atccgtcagc accgcacgac
     1861 gctcgtgttc gtgaacacgc gccggcacgc cgagcgcgcg gcgcgccact tggccgagcg
     1921 gctcggcgac gacgccgtca cgtcgcacca cggcagcctc gcgaaagagc atcgcttgaa
     1981 ggccgaggag cggctcaagg ccgggcagct caaggcgctc gtggcgaccg cgtcgctcga
     2041 gctcggtatc gacatcggcg acgtcgactt ggtctgccag ctcggctcgc cgcacggcat
     2101 ctcggcgttc ttgcagcgcg tcggccgctc gggccacggg ctcggccgcg ttccaaaagg
     2161 gcgcttgttc ccgctgtcgc gcgacgacct cgtcgagtgc acggcgctgc tcgcgagcat
     2221 ccagcgccgc gagctcgatg cgatagtcct aaggccgcag ccgctcgacg tgctcgcgca
     2281 gcagatcgtc gccgaggtcg gcgtgcagga gtggaaggtg caggacctgc accggcgctt
     2341 cacgcacgcc gcgacctaca aggatctccc gctcgaggag ttcgtcggcg tcgcgcgcat
     2401 ggtcgccg
//
LOCUS       DASSNN010000627         6491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_483440, whole genome shotgun sequence.
ACCESSION   DASSNN010000627 DASSNN010000000
VERSION     DASSNN010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483440"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..263
                     /locus_tag="VFL84_11715"
     CDS             120..263
                     /locus_tag="VFL84_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609337.1"
                     /translation="MLALPRHQIDGVVEQCWRIDTLADAGDLARATVPRTAGVLRSGR
                     ATH"
     gene            266..1291
                     /locus_tag="VFL84_11720"
     CDS             266..1291
                     /locus_tag="VFL84_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7609338.1"
                     /translation="MRLRSRAVGLALLAALFVFVAGCGRTSSGATEVRIPLGAGGVGF
                     LPLLVMRDLKLIEKHASAAGLASLRVRWIDLGGPAVMNDALLSGSVDFIAAGPPAFIT
                     LWDKTRGSADVRGVAAMSSLPMYLNTTNPKLTTLDDLTETDKIAVTAVKVSIPSIVMQ
                     MYAARRYGAAEAFRFDRYTVTMTHADALIALLSGANQIDAHFTSPPFHQREIEDARVR
                     TLLDTDDIMGGATTFTMLSTTARYREANPIAYAAVLAALEEANEIIRSDPRAAAEILF
                     AAESAAGFSVDELVEVLSDPDIRFTTTPENTEKYAEFMLSIGSIEHRPSSWRDLFFPE
                     VHGAAGS"
     gene            1461..3449
                     /locus_tag="VFL84_11725"
     CDS             1461..3449
                     /locus_tag="VFL84_11725"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HET7609339.1"
                     /translation="MASILAAGAIYYLATRAAWVMTFPDSKVSLFFPPHAILVSILLL
                     APTRHWWAYLLAAVCSHFVATQQTQWPPLYALQAEAYDAVTIALTAGAIRVLNKSPLH
                     LLSLRDAVVFVVIAVTLIPAGTAVWGAAFTVAYGYGTELWIEWRNLTISNAVTTIVLV
                     PAILIAVQQFKSGFKADRGRIVEAALLALGILAVGWLAFGGSPAGPDTSPALLYAPIP
                     LLIWAALRFGLGGMSASMLLITIFAIWGTMQGRGPFLAQAPAENAFALQLFILVAATP
                     LLLLSAVIADERRSKEAVRVTEQRMSLAAESAQLVLWDWDLAKDEVWIQDQGIFGFPP
                     DTAVDHATLGGSVHPDDRAVRDAAIQRALANGGHYESEFRVILRDGSVRWIAARGRSP
                     VTVIDGKPARILGVAIDITRQKQVAAEAQLQREELAHLSRVATMSALSGSLAHELNQP
                     LTSILANTYAGKNIASHDAPDLRELRDIFADIDSAAGRAGDIIARMRTLLRRGHVALQ
                     PVDVKESLVELLRLTRTDLIARGVSVTDLAIGDLPPVMSDRVQLQQILLNLIKNACDA
                     MQANAADDRKLTLTASVDQNELRIGVLDCGVGLPDDVESLFQPFHSTKEGGLGMGLSI
                     CRTLVSAHGGRLWAEGRASRGAAFYVALPLAGASATSS"
     gene            complement(3451..4722)
                     /locus_tag="VFL84_11730"
     CDS             complement(3451..4722)
                     /locus_tag="VFL84_11730"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET7609340.1"
                     /translation="MAQAARTELLETLQRLLEIPAADLKVALTQACDAIAAAVNADKV
                     DAFIYQPESDCLVAVGSSTQPLSAKQRRHGLDVLQVANGGRVVHVYQTGQTYVTGHLE
                     DDTEELRGIREALEIRSKIGVPLEVGGSRRGMIMIAALRPEAFTPEDVKFAEAVARWV
                     GAVAHRAELVQEIERNAVEQGRRLVAEELITVLAHDLRNYISTIEYRVRAIRRKAELE
                     GRDAEVRDCDAAQRSIGRLGSLVSDILDAARIDQGVLQLDPRPVELTPLVNEVIDTLG
                     TTQHRIVTKISEDLLVAADPKRVCQCLENILANAVQHSPDDAPVTVFIRRVKRENDDV
                     ARIDIVDEGPGIAPELLPRIFERFVTGKQRKGGLGLGLYLAKRIATLHNGDLTVESKL
                     GGGAQFTLLLPCYRDDVHVARRRTTGEQPEP"
     gene            complement(4815..5333)
                     /locus_tag="VFL84_11735"
     CDS             complement(4815..5333)
                     /locus_tag="VFL84_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609341.1"
                     /translation="MDRGALLTMHLELNAHERTDEYRRYFPDWTRRSLLLGEELEADH
                     NSYLAGGYAEEFGYLARAEASRGVPLIDGESADDRELRRVQRAGMHPGYPLRPHPVVL
                     GRKQAQRHRAISSGVARADSSETNDHGKFVTVTERAYEREHRQPAHRAVAAVTRFLAA
                     VVALGWELPSRR"
     gene            complement(5312..5779)
                     /locus_tag="VFL84_11740"
     CDS             complement(5312..5779)
                     /locus_tag="VFL84_11740"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7609342.1"
                     /translation="MKSRPSLRFDHVAVPVHDARSAYQLFAETLGLPLLAAHTGDDWG
                     GAPWLMMIFGLAQDGQVALCVRHGAGKARQPAVDLPHFALTTSGKAQLAEWERKLKAA
                     GFAIRHEDHGDQQSIYFDDRSGLTWEITTAREGSANADPDAHSTVDEWIAEHS"
     gene            5869..>6491
                     /locus_tag="VFL84_11745"
     CDS             5869..>6491
                     /locus_tag="VFL84_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609343.1"
                     /translation="MTFRRSILRRHFKFAALAAFALGAQAQRAPELVFDPTWPRDLPN
                     NWKLGGITGLALDRDENVWLYNRPNDLTNIELHAELDPPLALCCVRAPSMIHLDKAGR
                     VIGSFAAPQGHGMDVDSDGFVYLGQDTVRMYDPRTGQVVGEVARAPEREDGGPVGLQP
                     ASSRPGQGGRGPVAAFLPQPAPDPAAVAAQAERRAAFRAKYPPATPM"
BASE COUNT         1046 a         2218 c         2092 g         1135 t
ORIGIN      
        1 acatcgaagt cgtcttgcgc aacggccaaa cgttgtcgaa gttcgtcgag caatcgctag
       61 gcaacgtgca ccgccccttg agtaacgagt agctcaccga gaagttcgtc gaccaggccg
      121 tgctcgcgct accgcgccac cagatcgacg gcgtcgtcga gcagtgctgg cgcatcgaca
      181 cgcttgccga tgccggcgac ctcgcgcgcg caacggtgcc gcgcacggcc ggcgtactgc
      241 gctccgggcg cgcgacgcac tgagcatgcg cttgcgttcg cgcgccgtcg gtctcgcgct
      301 gctcgcggcg ctcttcgtgt tcgtcgcggg ctgtgggcga acgtcgagcg gcgccacgga
      361 ggtccgcatt ccgctcggcg cgggcggcgt cgggttcctg ccgctgctcg tgatgcgcga
      421 cctaaagctc atcgagaagc acgcgagcgc cgcgggcctc gcgagcttgc gcgtgcgctg
      481 gatcgatctc ggcggaccgg ccgtcatgaa cgacgcgctg ctgtcgggct cggtcgactt
      541 catcgccgcg gggccgcccg cgttcatcac cttgtgggac aaaacacgcg gctcggccga
      601 cgtgcgcggc gtcgcggcga tgtcgtcgct gccgatgtac ctgaacacga cgaacccgaa
      661 gctcacgacg ctcgacgatc tcacggagac cgacaagatc gccgtcacgg ccgtcaaagt
      721 ctcgatcccg tcgatcgtca tgcagatgta cgcggcgcgg cgctacggcg cggccgaagc
      781 gttccggttc gaccgctaca ccgtcacgat gacgcacgcc gacgcgctga tcgcgctgct
      841 ctcgggcgcc aaccagatcg acgcgcactt cacgtcgccg ccgtttcatc agcgtgagat
      901 cgaggacgcg cgcgtgcgca cgctgctcga caccgacgac atcatgggcg gcgcgacgac
      961 gttcacgatg ctgtccacga ccgcgcgcta ccgcgaggcc aaccccatcg cttacgcggc
     1021 agtgctcgct gcgctcgagg aagcgaacga gatcatccgc agcgacccgc gcgcggccgc
     1081 cgaaatcttg ttcgccgccg agtcggcggc cggattttcc gtcgacgagc tcgtcgaagt
     1141 cttgagtgac cccgacatcc gtttcacgac gacgcctgag aacaccgaga agtacgccga
     1201 attcatgctg tcgatcggct cgatcgagca tcgcccgagc tcgtggcgcg atctattctt
     1261 ccccgaggtc cacggggctg cgggctcata gccttcgagc gagattcggc ggcgccggcg
     1321 cgctcgccgt tgggcaacgc gcggaaatcc cttacccttg tcgggaaacc gggccgacag
     1381 aggcacacct caaggtgctc gcagacaacg tcgacgcgac agcccgcaag gacgccttgg
     1441 cgctcccctc agctcgcaca gtggcaagca ttctggcggc gggtgccatc tactatctcg
     1501 cgacgcgcgc cgcctgggtg atgacgttcc ccgacagcaa ggtctcgctg ttcttccctc
     1561 cgcacgccat cctggtttcg atcctgctgc tcgctcccac gcggcactgg tgggcctatc
     1621 tgctggccgc cgtgtgcagt cacttcgtcg cgacgcaaca gacgcaatgg ccgccgcttt
     1681 acgccctgca ggccgaggcc tatgacgcgg tcacgatcgc gctgacggcc ggcgccatcc
     1741 gcgtgctcaa caaatcgccg ctccatctcc tcagccttcg cgatgccgtc gtcttcgtcg
     1801 ttattgcggt gacgctaatc cccgccggta cggctgtgtg gggtgccgcg ttcaccgtcg
     1861 cgtatggcta cggcaccgag ctctggatcg aatggcggaa cctcaccatc tccaacgcgg
     1921 tgacgacgat agtgctggtc cccgcgatcc tgatcgcagt ccagcaattc aaatccggct
     1981 tcaaggctga tcgcggacgc atcgtcgaag cggctctcct cgccctcggc attctcgcag
     2041 tcggatggct cgcgttcggc ggctccccgg caggaccgga cacttcgccc gcccttctgt
     2101 acgcacccat tccgctgctg atctgggcgg cgctgcgctt tgggctggga gggatgagcg
     2161 cctcaatgct gctcatcacc atatttgcca tttggggcac gatgcaggga cgcggcccct
     2221 tcctggcgca agccccggcc gaaaacgcgt ttgccctgca gttgttcatc ctcgtggcgg
     2281 ccactccgct gttgctgctc tcggccgtca tcgctgacga aaggcgatcg aaggaggcgg
     2341 tgcgggtgac cgagcagcgc atgagcctcg cggcggagag cgcgcaattg gtgctgtggg
     2401 actgggatct ggcaaaggat gaagtctgga ttcaggacca gggtattttc ggcttccccc
     2461 cggacactgc cgtcgatcac gccacactgg gcggaagcgt ccatcccgac gatcgtgccg
     2521 tgcgcgacgc cgcgatccag cgcgcactgg caaacggcgg ccattatgaa tccgagtttc
     2581 gcgttatctt gcgcgacggc tcggtgcgat ggatcgcggc acgcggccgc agtccagtca
     2641 ccgtcatcga cggcaaaccg gcccgaatac tcggcgtcgc aatcgatatc acgcgtcaga
     2701 agcaagttgc cgcggaagcg cagctgcagc gcgaggagct cgcgcacctc tcgcgagtcg
     2761 ctacgatgag cgcgctatcg ggctcgcttg cgcatgagct gaatcagccg ctgacgagca
     2821 tcctcgccaa cacgtatgcg ggcaagaaca tcgcgtcgca cgacgcgccg gacttgcggg
     2881 agcttcgcga catcttcgcc gacatcgaca gtgccgccgg ccgcgccggc gacatcatcg
     2941 cacgcatgcg cacactgctg cgccgcggcc acgtcgcgct tcagcccgtc gatgtgaaag
     3001 agagcctcgt ggagctgctg cgcctcacgc gcacggatct cattgcgcgc ggcgtctccg
     3061 tgacggatct cgccatcggc gatcttcccc cggtcatgtc ggatcgcgtg caactccagc
     3121 aaatcctctt gaacctgatc aagaacgcgt gcgatgcgat gcaggctaac gcggcggacg
     3181 atcgcaagct cacgctcacg gcttcggtcg atcagaacga gctgcgcatc ggcgtgctgg
     3241 actgtggagt cggattaccc gacgacgtcg agtcgttgtt tcagccgttt cactccacga
     3301 aggaaggcgg gctcggaatg ggcctgtcca tctgccgcac gctggtgagc gcgcacggcg
     3361 gcaggctgtg ggcggaagga agggccagtc gcggtgccgc gttctacgtc gcgctgccac
     3421 tggcgggcgc aagcgcgacg tcgagctagg ctacggctcg ggctgctccc cggtcgtacg
     3481 tcgccgagcg acgtgcacgt cgtcgcgata gcacggcagc aacagcgtga actgagcgcc
     3541 cccgcctagc ttcgactcga ccgtgaggtc gccgttgtgc agcgtcgcga ttcgcttcgc
     3601 gaggtacagc ccgaggccga gcccgccctt gcgctgtttg ccggtgacga atcgctcgaa
     3661 gatgcgcgga agcagctcgg gtgcgattcc cggcccttcg tcgacgatgt cgatgcgagc
     3721 gacgtcgtcg ttttctcgct ttacgcggcg tatgaagacc gtcaccggcg cgtcgtccgg
     3781 cgagtgctgc acggcgttcg cgagaatgtt ttcgaggcat tggcacacgc gcttggggtc
     3841 ggcggcgacc agcaagtctt cggagatctt cgtcacgatg cgatgttggg tagtgccaag
     3901 ggtgtcgatc acttcgttga cgagcggcgt gagctccacg ggtctcgggt cgagctggag
     3961 cacgccttgg tcgatacgcg cggcgtccag aatgtcggac accagcgagc cgagccggcc
     4021 gatgctcctc tgcgccgcgt cgcagtcgcg cacctcggcg tcacgtcctt cgagctccgc
     4081 tttgcggcgg atcgctcgga ctctgtactc gatcgtcgaa atgtaattgc gtaggtcgtg
     4141 cgcgagcacg gtgatcagct cctcggcgac gaggcggcgg ccctgctcga cggcattgcg
     4201 ctcgatttcc tgtacgagct ccgctctatg cgcgactgcg ccgacccagc gcgcgacggc
     4261 ctccgcgaat ttcacgtcct cgggcgtgaa ggcttccggc cgcagcgcgg cgatcatgat
     4321 catcccgcgg cggctgccgc cgacctcgag cggaacgccg atcttcgacc gaatttccaa
     4381 cgcctcgcga attccgcgaa gctcctcggt atcgtcctcg aggtgccctg tcacgtaggt
     4441 ttgaccggtt tgatacacgt gaacgacgcg gccgccgttg gccacttgca ggacgtcgag
     4501 cccatgccgc ctctgcttcg cggacagcgg ctgagtgctc gagcctacgg ccacgagaca
     4561 atcgctttcg ggttggtaaa tgaaagcgtc gaccttgtcg gcgttcacgg ccgcggcaat
     4621 ggcatcgcac gcttgagtaa gcgccacctt gaggtcggcc gccggaatct ccagcaaccg
     4681 ctgcagcgtc tcgagcaact ccgtgcgagc ggcctgtgcc acggcatctc cttggcgtgg
     4741 ctggcacgcg ttggcgagcg gaataatcgc cgacacgcag caagaacaat gccacccgat
     4801 cgcggcggcc ggacctaacg tcgtgacggg agctcccaac ccaatgcgac gactgcggca
     4861 aggaatcgcg tgacagcggc gacagcccgg tgcgccggtt gtcgatgctc gcgctcataa
     4921 gctcgttcgg tcacggtgac gaacttgccg tgatcgttgg tctcggagct gtcggcgcgc
     4981 gccacgccgc tcgatatagc gcggtggcgc tgcgcttgct tccgacccag gacaaccgga
     5041 tgaggtctga gcggataacc ggggtgcatg cctgcccgct gaacccgccg cagctcgcgg
     5101 tcgtccgccg actccccgtc gatcagcggc acgccgcggg acgcctcggc ccgtgccaga
     5161 tagccgaatt cctcggcata gccgccggcg aggtaggagt tgtggtcggc ctcgagctct
     5221 tccccgagca gtagcgagcg acgagtccag tccggaaaat aacgccggta ctcgtccgtt
     5281 cgctcatgcg cgttcaattc aagatgcatg gtcaggagtg ctccgcgatc cattcgtcga
     5341 ccgtcgaatg cgcatccgga tcggcgttcg ccgagccctc gcgtgccgtc gtgatctccc
     5401 aggtgaggcc gctgcgatcg tcgaagtaga tcgattgctg gtcgccgtgg tcttcgtggc
     5461 gaatcgcgaa cccggccgct ttcagtttcc gttcccactc cgcgagctgc gccttgccgc
     5521 tcgtcgtcag cgcgaaatgc ggcagatcga ccgcaggttg ccgcgccttc cccgcgccgt
     5581 gccgcacgca caatgcaacc tgtccgtctt gcgcgagacc gaagatcatc atgagccacg
     5641 gcgcgccgcc ccagtcatcg cccgtgtgcg cggccagcag cggcagccca agcgtctcgg
     5701 cgaacaattg ataggcgctc ctcgcgtcgt gcaccggcac ggcgacatga tcgaagcgca
     5761 gcgacggccg gcttttcatt gcgtcagaat agccctcgcc ggccgaaccc gcacgcgcac
     5821 cgcgctacga ggccggcctg catggagttg cgctagcatg gcgctgccat gacgtttcgg
     5881 cgctcaatcc ttcgacggca cttcaagttc gccgcgctgg cggccttcgc gctcggcgcg
     5941 caggcgcagc gcgcgccgga gctcgtgttc gatccgacgt ggcccagaga cttgccgaac
     6001 aactggaagc tcggcggcat cacgggccta gcgctcgatc gcgacgagaa cgtctggctc
     6061 tacaaccgtc ccaacgatct cacgaacatc gagctgcatg ccgagctcga tccgccgctc
     6121 gcgctctgct gcgtgcgcgc gccgtcgatg attcacctcg acaaagcggg ccgcgtgatc
     6181 ggctcgttcg ccgcgccgca aggtcacggc atggacgtcg acagcgacgg cttcgtctat
     6241 ctcggccagg acacggtgcg catgtacgat ccgcggacgg gccaagtcgt cggcgaggtc
     6301 gcgcgcgcgc ccgagcgcga ggacggcggg cccgtcgggc tgcagcccgc gagctcgagg
     6361 cccgggcaag gcggccgcgg gccggtcgcg gcgttcctgc cgcaaccggc gccggatccc
     6421 gcggcagtcg ccgcgcaagc ggagcgccgc gcggcattcc gcgcgaagta tccgccggcg
     6481 acgccgatga t
//
LOCUS       DASSNN010000628         6307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1619830, whole genome shotgun sequence.
ACCESSION   DASSNN010000628 DASSNN010000000
VERSION     DASSNN010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619830"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFL84_11750"
     CDS             complement(<1..204)
                     /locus_tag="VFL84_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609344.1"
                     /translation="MNNNEPEVIATVARPLARSVAAGLVCLTAACWIAPASANVVTEW
                     NALALGCITRGGPAQAFDTSLVQA"
     gene            complement(442..5664)
                     /locus_tag="VFL84_11755"
     CDS             complement(442..5664)
                     /locus_tag="VFL84_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7609345.1"
                     /translation="MTAERMKIVLLAAMLASAGADAQLLREPSRGEAVERHLPGDRTF
                     TQWVLDPNAVNTSAGDRFEAREVAAEELETIKLTNLVPPIRFEVGVAEIPDATVVELR
                     RILESLRGRRNVRLHLVGHADNQPLGPALAAVFGDNEGLSRERAGEAAELLKGTLGLP
                     ADAVSYSWAGDREPVASNETAAGRAQNRRVEVEVWYDEVKQGTALDEVLVKEDFQRIK
                     VCRTEEVCRLRYVDGNERRTRVQNVVAPLRFAEEAIEVTPIYVEQIREAVAGLSDRHN
                     VLVKFIGYTDDSPLSERNARIYGDQVGLSRAQARRVALAVQQQLKLPTSAVDSDGRGS
                     SRPLGSNATAQGRALNRRVEVEFWYDDPLQDLPDEPQLCPAPGNELVTRIYDPPWGSL
                     PQLPIENGEPVVPAGLTGLLRRGLADVADKMNPRVRFIGYTRNERLERRTTLVYGDDV
                     GLSASRARRAMERIASEMQLLPTQVEFEGRGYVQSDDVVNSGFIQGDTSYVVAQVVYD
                     EIAQLDDYEGVEITPLTRELAPQNAFALNLMRITVDGKPLDDKDRSSADIQRCTDVAL
                     QEADIQFGFDDLEADRRLSVAADPATVDFYRGADGWAAEPVRFRMYANYSHFIDRAEV
                     RIFAPEQSLEAEPLAVVTFGPDGAGEWLPEPEMFAAPARELKYVLRAYGAGGTFDETV
                     PQPLWIAYREVGPIAELPLPSQSAPPAQDVDALLAEIATAGAPYVELPEVAPAAAAES
                     TLAESSVSADAGTTDRALRVAYGENGLGLRNIPLASGSVTVRGSAVPPGHAVYVAGRA
                     VPVAEDGSFVAQEILPSGVHTVEVAVLDQQGNGELYLRDIELQRKDWFYVGMADVTLT
                     SHDSNGPIELLQGENNDINFDSSADARLAFFINGKFNEHWRLTASADTRDAPLEDLFS
                     NFLDKSPESLLRRIDPDYHFPTFGDDGTVEEMAPTLGKFYVKVGRDDNYAQWGNFHVG
                     YMNNELAQVDRGLYGANLHYESQRATSFGERRFAIDGFTAEPGTVASREEFRGTGGSL
                     YFLQRQDLLVGSERVRIEYRDRASGLVTGVVNLTPAVDYEVDYLQGRLLLTEPLSATR
                     DDDLLVRDGAIQGDEAYLVVRYEYTPGFDELDALSTGAQGHYWFGERVKVGLTANAND
                     DGDADSGLEAADVTVRINSDSWFKLQGAQTEGFISTVMRSDDGGFGFSGYDGASFADA
                     DAGGYRADLSLSLGNFFDRARGRVTVYTQSLDGGYSAPGLQTLTDTENYGGTFQFPVT
                     DRLSVRAKSDTRSAELGLSSSAHELNVAYTLNENWGVATGVRKDERRDDSAVVPLTQE
                     QGQRTDAVVQVGYDSNRRWSAYGFVQDTLSVDGTREENARIGSGGSYRISERLKIDAE
                     VSNGDLGAGGKIGTSYLPNERTTLYMNYALENERTDNGLLSTRGREGNTVAGVKTRLS
                     DSTSVYLEERYQNSAFTSGLTHSTGISLAPTERFNLAASTDIGTLRDAVTGAETDRNA
                     AGFRIGYGFTALQLSSGVEYRHDETEQPDLTLNTRETWLLRNNFKWQLSDASRLLGKL
                     NYAESTSSLGAFYDGGYTEAVLGYALRPVRNDRLNALIKYTYFYNVPTTDQLTQRSIA
                     AEFIQKSHVGALDLTYDLTPKWSIGGKYAHRRGELSLTRDNPEFFDNTADLFVVRTDF
                     RFKEDWEGLVEVRALTMPDLSDKRSGALVVVSRYLNPHFKIGVGYNFTDFSDDLTDLS
                     YDHRGTFLSLTGAL"
     gene            complement(5661..>6307)
                     /locus_tag="VFL84_11760"
     CDS             complement(5661..>6307)
                     /locus_tag="VFL84_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609346.1"
                     /translation="PDPSLDCTDVIGKVFDDANRNGLQDEGERGIPQVRVATARGLVA
                     TTDPFGRFHITCAITPREDRGTNFVLKLDDRTLPSGYRGSTELMQMKRATRGKALEFN
                     FGASIHRVIGLDLADPVFEPGSTQIRELWLPRLELLMSELRAAPAVLRLSYLADVEEP
                     QLVDARLKALRERIDTAWHATENCCAYDLVVEPEVFWRRGEPTGARERRRGERP"
BASE COUNT          959 a         2124 c         2141 g         1083 t
ORIGIN      
        1 ggcctggacg agtgaggtgt cgaacgcttg cgccggcccg ccgcgtgtaa tgcagccgag
       61 cgcgagcgcg ttccattcgg tcactacgtt cgcgctcgcg ggcgcgatcc agcatgccgc
      121 ggttaggcag accaacccgg cagcgaccga tcgcgcgagc ggacgcgcaa cggtcgcaat
      181 gacttcaggt tcattattgt tcactttgat ctcctgacag gtattggtgg taacgggaat
      241 cttttcagcg tcgagctttt tgcgtcacga gcgaacgctg agagggtcac tagcgaagca
      301 tgagaattgc gattcgggtt tcaagagtgc ccgaagcgag acagatgtca actacggaat
      361 gacgtgcgaa acggttgttt ttgtttgttg ctcgacgcaa ccgagcacta cacgttcgcg
      421 aaccgaagcc aaacgactgc gctacaacgc gcccgtcaag ctcaagaatg tgccgcgatg
      481 gtcgtagctt agatccgtga ggtcgtcgga aaagtcggtg aagttgtagc cgacgccgat
      541 tttgaagtgc gggttcaagt agcgcgacac gacgacgagc gcgccgctgc gcttgtcgga
      601 gaggtccggc atcgtcaacg cgcgcacttc gacgagccct tcccagtctt ccttgaacct
      661 gaaatccgtc cgcacgacga acaagtcggc cgtgttgtcg aagaactcgg ggttgtcgcg
      721 cgtgaggctc agctcgccgc gccggtgcgc gtacttgccg ccgatcgacc acttcggcgt
      781 gaggtcgtag gtcaggtcga gcgcgccgac gtggctcttc tggatgaact cggccgcgat
      841 gctgcgctgc gtcagctgat ccgtcgtcgg cacgttgtag aagtacgtgt acttgatcag
      901 cgcgttgagc cggtcgttcc ggaccgggcg cagcgcgtag ccgagcacgg cttcggtgta
      961 gccgccgtcg tagaacgccc cgagcgagct cgtgctctcg gcgtagttca gcttgccgag
     1021 caagcgcgac gcgtccgaca gctgccactt gaagttgttg cgcagcagcc aggtctcgcg
     1081 cgtgttcagc gtcagatccg gctgctcggt ctcgtcgtgg cgatactcga cgccgctcga
     1141 gagctgcagc gcggtgaagc cataaccgat cctaaagccg gccgcgttgc gatccgtctc
     1201 cgcgcccgtc accgcatcgc gtagcgtgcc gatgtcggtg ctcgcggcca ggttgaaccg
     1261 ctccgtcggc gccaagctga tgccggtcga gtgcgtgagg cccgacgtga atgcgctgtt
     1321 ctgatagcgc tcttcgaggt acacgctcgt gctgtcggac aatcgcgtct tgacgccggc
     1381 cacggtgttg ccttcgcgcc cgcgcgtcga aagcaggccg ttgtccgtgc gctcgttctc
     1441 gagcgcatag ttcatgtaca gcgtcgtgcg ctcattcggc agatagctcg tgccgatctt
     1501 gccgcccgcg ccgagatcgc cgttcgagac ttcggcgtcg atcttcaggc gctccgagat
     1561 tcgatacgag ccgcccgacc cgatgcgcgc gttctcctcg cgcgtgccgt ccaccgacag
     1621 cgtgtcctgc acgaaaccgt acgcgctcca gcggcggttc gagtcgtagc cgacttgcac
     1681 gacggcgtcg gtgcgctgac cctgctcttg cgtcagcggc accaccgccg agtcgtcgcg
     1741 gcgctcgtcc ttgcgcacgc cggtcgcgac gccccagttc tcgttgagcg tgtatgcgac
     1801 gttgagctca tgcgcgctcg agctaaggcc aagctcggcg ctgcgcgtgt cggacttcgc
     1861 gcgcaccgac agccgatcgg tcacggggaa ctggaacgtg ccgccgtagt tctcggtgtc
     1921 ggtgagcgtc tgcaggcccg gcgccgagta gccgccatcc aagctctgcg tgtagacggt
     1981 cacgcggccg cgcgcgcggt cgaagaagtt gccgagcgag aggctcaggt cggcgcgata
     2041 gccgccggcg tccgcgtccg cgaacgaggc gccgtcgtag cccgagaagc cgaagccgcc
     2101 gtcgtcggag cgcatgacgg tggagatgaa gccttccgtc tgcgcgcctt gcagcttgaa
     2161 ccacgaatcc gagttgatgc gcaccgtcac gtcggccgct tcgagcccgc tgtcggcgtc
     2221 gccgtcgtcg ttcgcgttcg ccgttaggcc gaccttgacg cgctcgccga accagtagtg
     2281 gccttgcgcg cccgtcgaca aagcgtcgag ctcgtcgaat ccgggcgtgt attcgtagcg
     2341 cacgacgagg tacgcctcgt cgccctggat cgcgccgtcg cgcacgagca agtcgtcgtc
     2401 gcgcgtcgca ctgagcggct ccgtcagcag cagccggcct tgcaggtagt cgacctcgta
     2461 gtcgacggcc ggcgtcaagt tcacgacgcc cgtgacgagc cctgaggcgc ggtcgcgata
     2521 ctcgatgcgc acgcgctccg agcccacgag caagtcctgg cgctgcagga agtacagcga
     2581 cccgcccgtg ccgcggaatt cctcgcggct cgccaccgtg ccgggctccg ccgtgaaacc
     2641 gtcgatggcg aagcgccgct cgccgaagct cgtcgcgcgc tgcgattcgt aatgcaggtt
     2701 cgcgccatag agcccgcggt cgacttgcgc gagctcgttg ttcatgtagc cgacgtggaa
     2761 gttgccccat tgcgcgtagt tgtcgtcgcg cccgactttg acgtagaact tgccgagcgt
     2821 cggcgccatc tcctcaaccg taccgtcgtc gccgaacgtc gggaagtgat agtcgggatc
     2881 gatgcggcgc aacagcgatt ccggcgactt gtcgaggaag ttgctgaaca gatcctcgag
     2941 cggcgcgtcg cgcgtgtcgg cgctcgccgt caaccgccag tgctcgttga acttgccgtt
     3001 gatgaaaaac gcgaggcgcg cgtcggccga ggaatcgaag ttgatatcgt tgttctcgcc
     3061 ttgcagcagc tcgatcgggc cgttcgagtc gtggctcgtc agcgtgacgt cggccatgcc
     3121 gacatagaac cagtccttgc gctgcagctc gatgtcgcgc aggtagagct cgccgttgcc
     3181 ctgctggtcg agcacggcca cttcgaccgt gtgcacgccc gacggcaaga tctcctgcgc
     3241 gacgaagctg ccgtcctcgg cgaccggcac ggcgcggccc gcgacgtaga ccgcgtggcc
     3301 cggcgggacc gcgctgccgc gcacggtcac ggagccgctc gcgagcggaa tgttgcgaag
     3361 ccccaagccg ttctcgccgt acgcgacgcg caaggcgcgg tccgtcgtgc cggcgtccgc
     3421 tgagaccgaa gactccgcta aagtggactc cgctgccgca gccggcgcaa cctcgggcag
     3481 ctcgacatac ggcgccccgg ccgtcgcgat ctcggcgagc agcgcgtcga cgtcttgcgc
     3541 gggcggcgcc gactgcgacg gcaacggcag ctcggcgatc gggccgacct cgcgataggc
     3601 gatccagagc ggctgcggca ccgtctcgtc gaacgtgccg cccgcgccgt acgcgcgcag
     3661 cacgtacttg agctcacgcg ccggcgccgc gaacatctcg ggctccggca gccactcgcc
     3721 ggcgccgtcg gggccgaacg tgacgaccgc gagcggctcc gcctcgagcg actgctcggg
     3781 cgcaaagatg cgcacctcgg cgcggtcgat gaagtgcgag tagttcgcgt acatgcggaa
     3841 gcgcacgggc tccgcggccc agccgtcggc gccgcggtag aagtcgaccg tcgccgggtc
     3901 cgccgcgacc gagagccgcc ggtcggcctc gagatcgtcg aagccgaact ggatgtcggc
     3961 ctcctgcagc gcgacgtcgg tgcagcgctg gatgtcggcg gagctgcgat ccttgtcgtc
     4021 gagcggcttg ccgtcgacgg tgatgcgcat caagttcaac gcgaacgcgt tctgcggggc
     4081 gagctcgcgc gtcagcggcg tgatctcgac gccttcgtag tcgtcgagct gcgcgatctc
     4141 gtcgtagacg acttgcgcga cgacgtacga cgtgtcgccc tggatgaacc ccgagttgac
     4201 gacgtcgtcg gactgaacgt agccgcggcc ctcgaactcg acctgcgtag gcaggagctg
     4261 catctcggag gcgatccgct ccatcgcgcg gcgggcgcgc gacgccgata ggccgacgtc
     4321 gtcgccgtag acgagcgtcg tgcgccgctc gagccgctcg ttgcgcgtgt agccgatgaa
     4381 gcgcacgcgc gggttcatct tgtccgcgac gtcggctaaa ccgcggcgca ggagaccggt
     4441 gagcccggcc ggcacgaccg gctcgccgtt ctcgatcggg agctgcggca acgatcccca
     4501 cggcgggtcg tagatgcgcg tgacgagctc gttgccgggc gccgggcaga gctgcggctc
     4561 gtcgggcagg tcctgcagcg gatcgtcgta ccagaattcc acctcgacgc ggcggttcag
     4621 cgcgcgcccc tgcgccgtcg cgttcgagcc gagcggcctc gagctgccgc gcccgtcgct
     4681 gtcgacggcc gacgtcggga gcttcaattg ctgctgcacg gccagcgcga cgcggcgcgc
     4741 ctgcgcacgc gacaggccga cctggtcgcc gtagatgcgc gcgttgcgct cggacagcgg
     4801 cgagtcgtcg gtgtagccga tgaacttgac gagcacgttg tgccggtcgc tcaagccagc
     4861 gacggcctcg cggatctgct cgacgtagat cggcgtgacc tcgatcgcct cttcggcgaa
     4921 gcgcaacggc gcgacgacgt tctgcacgcg cgtgcgccgc tcgttgccgt cgacgtagcg
     4981 cagccggcaa acttcctccg tccggcagac cttgatgcgc tggaagtcct ccttgacgag
     5041 cacctcgtcg agcgccgtgc cttgcttgac ctcgtcgtac cagacctcga cctcgacgcg
     5101 gcggttctgc gcgcggccgg ctgccgtctc gttcgacgcg acgggctcgc gatcgccggc
     5161 ccacgagtac gagaccgcgt cggccggcag ccccagcgtg cccttcaaga gctcggcggc
     5221 ctcgccggcg cgctcgcgcg acaagccttc gttgtcgccg aacacggccg cgagcgccgg
     5281 tccgagaggc tggttgtccg cgtgacccac gaggtgcagt cgcacgttgc gccgaccgcg
     5341 aaggctctcg aggatcctgc gcagctcgac gaccgtcgcg tcggggatct ccgcgacgcc
     5401 gacctcgaag cgaatcggcg gcacgagatt cgtgagcttg atcgtttcga gctcctcggc
     5461 cgccacttcg cgcgcctcga accggtcgcc cgcggacgtg ttcactgcgt tcgggtcgag
     5521 aacccattga gtgaacgtgc ggtcgccggg caagtggcgc tcgacggctt cgccgcgcga
     5581 cggctcgcgc agcaactgcg cgtcggcgcc cgcgctcgcg agcatcgcgg cgagcagcac
     5641 gattttcatg cgctcggccg tcatgggcgt tctcccctgc gccgctcgcg tgcaccggtg
     5701 ggctcgccgc ggcgccagaa cacctcgggc tcgacgacga ggtcgtaggc gcagcagttc
     5761 tccgtggcat gccacgccgt gtcgattcgc tcgcgcagcg ctttcaggcg cgcgtcgacg
     5821 agctgcggct cctcgacgtc ggcgagatac gagaggcgca gcacggcggg cgcggcgcgc
     5881 agctcgctca tcaagagctc gaggcgcggc agccacagct cgcgaatctg cgtggagccc
     5941 ggctcgaaca cggggtccgc gagatcgagg ccgatgacgc ggtggatcga cgcgccgaag
     6001 ttgaactcga gcgccttgcc gcgcgtcgcg cgcttcattt gcatcagctc ggtcgagccg
     6061 cggtagccgc tcggcagcgt ccggtcgtcg agcttcagca cgaagttcgt gccgcggtct
     6121 tcgcgcggcg tgatcgcgca cgtgatgtgg aagcggccga atggatccgt cgtcgcgacc
     6181 aagccccgcg ccgtcgcgac gcgaacctgt ggaatgccgc gctcgccttc gtcctgcaag
     6241 ccgttgcggt tcgcgtcgtc gaagaccttg ccgatgacgt cggtgcaatc gagcgacggg
     6301 tccggaa
//
LOCUS       DASSNN010000629         2180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1619831, whole genome shotgun sequence.
ACCESSION   DASSNN010000629 DASSNN010000000
VERSION     DASSNN010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619831"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VFL84_11765"
     CDS             <1..687
                     /locus_tag="VFL84_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET7609347.1"
                     /translation="EIGDMSPALQTRLLRVLAESEFFRVGGQTPIRVDVRVIAATHQD
                     LKQAVAAGRFREDLYHRLNVMRIEVPPLRQRREDIPELARFYLERAANELGVAPKTLS
                     PAAAALLLRYDWPGNVRELVNVARRLTVTAAGREIVEHDLPRDLGGLAAEPNADWTAE
                     LAQWAETQLAAGDGLPLLAEALPAFERTLIVAVLRRVRGRRLEAAKLLGWGRNTLTRK
                     IKELGLDGRV"
     gene            complement(694..1656)
                     /locus_tag="VFL84_11770"
     CDS             complement(694..1656)
                     /locus_tag="VFL84_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7609348.1"
                     /translation="MKTLRTAVVGVGYLGRFHAEKHRTLPAVELVGVADVDAAAGQSV
                     AERLGTHYFADHRELAGAVDAVTIAAATSAHFELARFFLEHGVHVLVEKPMTETSSEA
                     RILTELAEARGLKLQVGHVERFNPALLSVQERLTNVQFIECHRLAPFKPRGADVSVVL
                     DLMIHDLDVVLSLVDAEPASVSAVGIKVLTESADIANARIEFDSGAIANLTASRVSTS
                     AQRKFRVFEQNQYVSIDFGEGEVQRVVSQGAVHEGPPLAIERSRLDKGDALLAETEAF
                     VTAVLEDKPCRVSGRDGLAALELAEAILADIERRAARGPRDRAG"
     gene            complement(1653..>2180)
                     /locus_tag="VFL84_11775"
     CDS             complement(1653..>2180)
                     /locus_tag="VFL84_11775"
                     /inference="COORDINATES: protein motif:HMM:NF025097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET7609349.1"
                     /translation="DNRIGEFATVSLGTEKGGGVTRIGHRNMLMAYVHVGHDCQLGNE
                     VILTNTCQLAGHVVIEDRALVSGHTGVVQFVRLGTCSFLVAGSFTNKDIPPFTIAEGH
                     WATLRAVNKVGLRRAGFDLEERGNIERAVRLLLDRGATIEEVVRRIGEECAETPAVRH
                     LVEFLSTSKKGVARR"
BASE COUNT          337 a          757 c          737 g          349 t
ORIGIN      
        1 gagatcggcg acatgtcgcc ggcgctccag acacggctgc tgcgcgtgct ggccgagagc
       61 gagttcttcc gcgtcggagg gcagacgccc attcgggtcg acgtgcgcgt catcgcggcc
      121 actcatcagg atctgaagca agccgtcgcg gccggccgct tccgcgagga cctttatcat
      181 cgcctcaacg tcatgcgcat cgaggtgccg cctctgcgcc agcggcgcga agacattccc
      241 gagctcgcgc gcttttatct cgagcgcgcc gcgaacgagc tcggcgtcgc gcccaagacc
      301 ctgagtcccg ccgccgcggc actgctcttg cgctacgact ggccgggcaa cgtccgcgag
      361 ctcgtcaacg tcgcgcgccg gctcacggtc acggccgccg gacgcgagat cgtggagcac
      421 gatttgccgc gcgacctcgg cggcctcgcg gccgagccga acgcagactg gacggccgag
      481 ctcgcgcaat gggccgagac ccaactcgcc gcgggcgacg gcctgccgtt gctcgccgag
      541 gcgctgccgg ccttcgaacg caccctcatc gtcgccgtcc tgcgccgcgt gcgcggcagg
      601 cggctcgaag ccgcgaagct gctcgggtgg gggcgcaata ccctcacgcg taagatcaaa
      661 gagctgggcc tcgacggccg cgtctagccg cgtttatccg gcgcgatctc gaggcccgcg
      721 cgccgcccgg cgctcgatgt ctgcgaggat ggcttcggcg agctcgagcg cggcgaggcc
      781 gtcgcgcccc gacacgcggc agggcttgtc ttcgagcacg gccgtaacga aagcctccgt
      841 ctcggccagc agcgcgtcgc ccttgtcgag ccgcgagcgc tcgatggcga gcggcggacc
      901 ttcatgcacg gcgccctgac tcacgacgcg ctgcacctcg ccctcgccga agtcgatgga
      961 gacgtattgg ttctgctcga agacgcggaa cttgcgctgt gccgaggtcg agacccgcga
     1021 agcggtgaga ttcgcgatcg cgccgctgtc gaactcgatg cgcgcgttcg cgatgtcggc
     1081 gctttccgtc agcaccttga tgccgaccgc cgagacgctc gcgggctcgg cgtcgacgag
     1141 cgacaggacg acgtcgagat cgtgaatcat gaggtcgagc accacgctca cgtcggcgcc
     1201 gcgcggcttg aacggcgcga gccgatgaca ctcgatgaac tgcacgttcg tgagccgctc
     1261 ctgcacggaa agcagcgccg gattgaaacg ctcgacatgc ccgacctgca gcttgagccc
     1321 gcgcgcctcg gcgagctcgg tcaggatgcg cgcttcgctc gacgtctcgg tcatgggttt
     1381 ctccacgagc acgtgcacgc cgtgctcgag gaagaaacgc gcgagctcga aatgggcgct
     1441 cgtcgcggcc gcgatggtga cggcgtcgac cgcccccgcg agctcgcgat ggtcggcgaa
     1501 gtagtgcgtg ccgaggcgct cggcgacgct ttggcccgcc gccgcatcga catcggcgac
     1561 gccgacgagc tcgacggccg gcagagtgcg gtgtttctcg gcatggaagc ggccgagata
     1621 gccgacgccg acgaccgcgg ttcgcagagt cttcatcttc tcgcgacgcc tttcttcgag
     1681 gtactaagga actcgacgag atggcgcacc gcgggggtct cggcgcactc ttcgccgatc
     1741 ctgcgcacga cttcctcgat cgttgcgccg cggtcgagca gcagcctgac cgcccgctcg
     1801 atgttcccgc gttcctcgag atcgaaaccc gcgcgcctga ggcccacctt gttcaccgcg
     1861 cgcagcgtgg cccaatgccc ttcggcgatc gtgaagggcg gaatgtcctt gttcgtgaag
     1921 gagcccgcga cgagaaagga gcaggtgccg agccgcacga actgcacgac cccggtgtgg
     1981 ccgctcacga gcgcgcgatc ttcgatgacg acgtgcccgg cgagctgaca ggtgttcgtg
     2041 aggatcacct cgttgccgag ctggcaatcg tgtcccacgt gcacataggc catgagcatg
     2101 ttgcgatgcc cgatgcgcgt gacgccgccg cctttctcgg tgccgaggct gacggtcgcg
     2161 aattcgccga tgcggttgtc
//
LOCUS       DASSNN010000630         3087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_610737, whole genome shotgun sequence.
ACCESSION   DASSNN010000630 DASSNN010000000
VERSION     DASSNN010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610737"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VFL84_11780"
     CDS             <1..1074
                     /locus_tag="VFL84_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7609350.1"
                     /translation="LKVIERNARAQAQIIEDLLDMSSIISGKVRLEMRAVDLPSVLEA
                     SVNAVRPAAEAKGIELALALDATTHALRADPNRLQQVFWNLLTNAVKFTPKGGRVSVT
                     LERVNAHMKVTVADNGEGIDAAFLPYIFERFRQADASASRRHGGLGLGLSIVKQLVEL
                     HGGSINASSDGNGAGSTFTVELPAMALELDAAGLSAPRHQSSRSMAEQSDAYARTADL
                     GGVRVLVVDDEPDARSLIERLLEDCDATVTTAASASEALEHVAREAPDVLLSDIGMPA
                     EDGYSLMRRIRNLTGEASRVPAIALTAYARSEDRDKALQAGYQMHLAKPVEPVKLIEM
                     VASLVTRRRRYPPNVRERGSPSS"
     gene            complement(1117..1482)
                     /locus_tag="VFL84_11785"
     CDS             complement(1117..1482)
                     /locus_tag="VFL84_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET7609351.1"
                     /translation="MPTATGHTTAIRAGKVIGTDVYDRQGKKIGEVKDIVLEKTSNNI
                     LFAIVSFGGVLGMGEKYHPVPWAELDYDPERGGYTVSFTAEQLKNAPADSIEALTKGD
                     GRAFRDRAFSHYGTKPYWH"
     gene            complement(1581..1655)
                     /locus_tag="VFL84_11790"
     tRNA            complement(1581..1655)
                     /locus_tag="VFL84_11790"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1621..1623),aa:Thr,seq:tgt)
     gene            complement(1751..2530)
                     /locus_tag="VFL84_11795"
     CDS             complement(1751..2530)
                     /locus_tag="VFL84_11795"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7609352.1"
                     /translation="MRHLFLMLVLMNLAFAAWTAWFAPSERAGRPADEGLPALTLVSE
                     VPADLHSGAVADEGEAPSLPADAAPTDGVADAESGAAAPAPGEEAVVTSERPAEPEAR
                     CTSVGPFRELSQAAAAAATLRGEGFEPQQRVAEGEVWSGYWVYIPAIPTEAEANETLA
                     KVREGVREARIPDAYVIRNSDSGNLVSLGVFSEISGVSRLRDEVRALGFEPQVVDRMS
                     RATVYWIDIALAPGQALDFEALQPPGRIIRLEQRACDRAVN"
     gene            complement(2530..>3087)
                     /locus_tag="VFL84_11800"
     CDS             complement(2530..>3087)
                     /locus_tag="VFL84_11800"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET7609353.1"
                     /translation="ALVATRPGTSLTLVATAAEQFGVRCAYAEPHRLGVDRWVAVLAA
                     YHAARGAACAIDAGTAVTFDAVDATGAHLGGLILPGARLLGAALDRNTSNIGTTAAAS
                     GVARGLELLGRNTDVAVGNAAWLALSAALDRAVATVEHALGARPVVYLTGGDAEPLRA
                     WLETRVELRADLVLEGLKLFAAEES"
BASE COUNT          500 a         1063 c         1035 g          489 t
ORIGIN      
        1 ctcaaggtga tcgagcgcaa tgcgcgggcg caagcgcaaa tcatcgagga cctgctcgac
       61 atgagcagca tcatctccgg caaggtgcgc ctcgagatgc gcgccgtcga cctgccgtcc
      121 gttctggagg cctcggtcaa cgccgtgcga cccgccgccg aagcgaaggg catcgagctc
      181 gcgctcgctc tcgatgcgac gacgcacgcc ctgcgcgccg atcccaatcg gctgcagcaa
      241 gtgttctgga acctgttgac gaacgccgtc aagttcacgc cgaaaggcgg acgcgtgtcc
      301 gtgacactcg agcgagtgaa cgcgcacatg aaggtcaccg ttgcggacaa tggcgaaggc
      361 atcgacgcgg cgttcttgcc ttacatcttc gaacgttttc ggcaggccga cgcctccgct
      421 tcgcgccgcc acggcgggtt gggcctcggg ctctcgatcg tcaagcagct cgtcgagctg
      481 catggcggca gcatcaatgc ttccagcgac ggcaacggcg ccggatcgac gttcaccgtc
      541 gagctgcccg caatggcgct cgagctggac gcggcgggac taagtgcacc gcggcatcag
      601 tcctcgcgtt caatggcgga gcaaagcgac gcttacgcgc ggacggccga tctcggcggc
      661 gtccgcgtcc tcgtcgtcga cgacgagccc gacgcgcgct cgctcatcga gcgcttgctc
      721 gaggactgtg acgcgaccgt cacgacggcc gcgtcggcga gcgaagctct cgagcacgtt
      781 gcgcgcgaag ctccggacgt tctgctcagc gacatcggca tgccggcgga agacggctac
      841 agcttgatgc gccgcatcag aaacctcacc ggcgaggcga gccgtgtccc ggcgatcgct
      901 ttgacggcct acgcgcgcag cgaggaccgc gacaaagcgt tgcaagcggg ctatcaaatg
      961 catctcgcga agcccgtcga gccggtcaag ctcatcgaga tggtcgcgag cctcgtgacg
     1021 cggcgacggc ggtatccgcc gaacgtgcgc gagcggggct cgccttcgtc ttagagaagg
     1081 ctgcacgccc gccgagcggg cgtgcgcctc aggacgtcag tgccagtagg gcttggttcc
     1141 gtagtgcgag aacgcgcggt cgcggaacgc tcggccgtcg cccttcgtca aggcttcgat
     1201 cgagtccgcg ggagcgttct tcagctgctc ggccgtgaac gagaccgtgt agccgccccg
     1261 ttccggatcg tagtcgagct cggcccaggg cacggggtgg tatttctcgc ccattccgag
     1321 cacgcccccg aagctcacaa tcgcgaacaa gatgttgttg gaagttttct cgagcacgat
     1381 gtccttgacc tcgccgatct ttttcccttg tcgatcgtag acgtcggtgc cgatgacctt
     1441 gccggcgcgg attgcagtcg tgtgtcccgt agcagttggc atggttcgtt cctccttgtc
     1501 gaaacggccg ttttgatgca cgcagtgtgc caatcgggca agaccgctgc gccgagcttt
     1561 cggagacgcc acgactgaga tggtgccggt ggagagaatc gaactcccga ccttcggttt
     1621 acaaaaccgc tgcactaccg ctgtgctaca ccggcgctcg gtatctcaaa tcgcgctcga
     1681 cgagcgcaga tgttgtgcgc ggcattatag ccccgcgcgg cgcgcatcat cgtgctccga
     1741 cggcggcagc ctagttcacc gcgcgatcgc aggcgcgctg ctcgagcctg atgatccggc
     1801 ccggcggctg cagcgcctcg aagtcgagcg cctggccggg cgcgagcgcg atgtcgatcc
     1861 agtagacggt cgcgcggctc atgcgatcga cgacttgcgg ctcgaagccg agcgcgcgga
     1921 cctcgtcgcg cagccggcta accccgctga tctcgctgaa cacaccgagc gaaacgagat
     1981 tgccgctgtc gctattgcga atgacgtaag cgtccgggat gcgcgcctcg cgcacgcctt
     2041 cgcgaacctt cgcgagtgtt tcgttggcct cggcctcggt cggaatcgcc ggaatgtaga
     2101 cccaatagcc gctccagacc tcgccttcgg ccactcgctg ctgcggctca aagccttcgc
     2161 cgcgcagcgt cgcggcggcg gcagcggcct gcgacagctc gcgaaaaggg ccgacgctcg
     2221 tgcagcgggc ttcaggctcg gcggggcgct ctgaagtcac gaccgcttct tcgccgggcg
     2281 caggcgcagc cgcgccggat tcggcatccg ctacgccgtc cgtcggcgca gcgtcggcgg
     2341 gtagcgaggg cgcttcgccc tcgtccgcga cggcgccgct gtgcagatcg gccggcacct
     2401 cgctgacgag cgtcagcgcc ggcagccctt cgtcggccgg gcgccccgcg cgctcggacg
     2461 gcgcgaacca tgcggtccac gcagcgaaag cgagattcat cagaacgagc atgagaaaga
     2521 gatggcgcat cagctctctt ccgcggcaaa cagcttgagc ccttcgagca ccaagtctgc
     2581 gcgcagctcg acgcgggttt ccagccaggc ccgcagcggc tcggcgtcgc cgcccgtcaa
     2641 gtacacgacc ggccgcgcgc cgagcgcatg ctcgacggtc gcgacggcgc gatcgagcgc
     2701 ggcggacagt gccagccacg cggcattccc tacggccacg tccgtgttgc ggccgagtag
     2761 ctcgagcccg cgcgcgacgc cggacgccgc ggccgtcgtg ccgatgttgc tcgtgttgcg
     2821 atcgagcgcg gcgccgagca ggcgcgcgcc cggcaagatc aaccctccga gatgcgcgcc
     2881 ggtcgcgtcg acggcgtcga acgtgaccgc cgtgcccgcg tcgatcgcgc acgcggcgcc
     2941 gcgcgccgcg tgatacgccg cgagcacggc gacccagcgg tcgacgccga gccggtgcgg
     3001 ctccgcgtac gcgcagcgga cgccgaactg ctcggcggca gtcgcgacga gcgtgagcga
     3061 cgtccccggc cgagtcgcga cgagcgc
//
LOCUS       DASSNN010000631         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_848004, whole genome shotgun sequence.
ACCESSION   DASSNN010000631 DASSNN010000000
VERSION     DASSNN010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848004"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..>2752)
                     /locus_tag="VFL84_11805"
     CDS             complement(94..>2752)
                     /locus_tag="VFL84_11805"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7609354.1"
                     /translation="DTELSWFVDLTVTRELSAVPGMGRVTRVGGVAREIRVDLDPDRM
                     AALGATASDVSRQLRRIQAEYPGGEGRLGGLEQTVRTTGIIDNVYDLRGLPISLPDGR
                     SVRLDTIAEVRDQAGEQRAAALLDGKPVIGFEIVRSWGASALDVAKEARVVVERLQKE
                     YPNVHFAEASSTVDYIQESFDASMEMLAEGAVLAIIVVFLFLRDWRATAVSAVALPLS
                     IIPTFWAIWLLGYTLNILTLLALSLVVGILVDDAIVEVENIVRHLRMGKKPREAAMDA
                     AIEIGLAVVATTLTICAVFIPVAFMSGIAGEFFGPFSFTVVVAVLCSRLVARTLTPMM
                     AAYFLKPHDRPERPARIMTWYLERVRWCLAHRWTTLGVASVAIGAMLGLAPFVSTAFS
                     PAGSNGFTTLSVELAPGSSLEDTLAVAEAARKRLGQLPEVRSVYTTVGAQGGGGGRNP
                     TGGGSAGNVRRGTMVIQLDNPEGTRGAQQIFESKATELLRDIPGARFQFQGSGGSSLE
                     VTLVGDEPNRLNLAATNVEREIRSLPGLGMITSSAALQKPEIVIRPDPERAAELGVTT
                     ETLSLVTRIATSGDVNTSLSKFNLDNRQIPILVRLNDKSRSDLERLENLSVPGRNGPV
                     PLANVADVSLGAGPAQITRVDRSRKITLNVNLNGQALGDMLDAIEALPAMRNLPDGVR
                     LVPTGDSRWIREIFGEFALAMAIGVLAIYAVLVMLFHKFIQPVTILAALPPSAAGAIL
                     ALFLSGSDLAINTLIGLLMLMGIVSKNSILIVEYAIMAQRDQGMSRFDSLIDSCSKRA
                     RPIVMTTIAMIAGMTPIAMGWAGDPSFRAPMGIAVIGGLIVSTIMSLFIVPAVFTVVD
                     DFQVWLSRLWRRGEAGDDADKMPAPSAVG"
BASE COUNT          460 a          935 c          938 g          419 t
ORIGIN      
        1 cgggcgtagt ctggaactac gcggtcagca gcacctatac gtggggcaga ccgaagcagt
       61 cctgacgtcg ctccatttcg ccgcgctacg cgctcaacca acggcgctcg gcgccggcat
      121 tttgtcggcg tcgtcaccgg cctcgccgcg ccgccagagc cgcgagagcc aaacctggaa
      181 gtcgtcgacg accgtgaata ccgcgggcac gatgaacaag ctcatgatcg tcgagacgat
      241 caagccgccg atgacggcga tacccatcgg cgcgcggaag ctcgggtcgc cggcccagcc
      301 catcgcgatc ggcgtcatgc ccgcgatcat ggcgatcgtc gtcatgacga tcggccgcgc
      361 gcgcttgctg cacgagtcga tcagcgagtc gaaccgcgac atgccctgat cacgctgcgc
      421 catgattgcg tactcgacga tcaggatcga gttcttcgac acgatgccca tcagcatcaa
      481 caagccgatc agcgtgttga tcgcgaggtc gctaccggac aagaacagcg ccaggatggc
      541 gccggccgcc gacggcggca gcgcggccag gatcgtcacg ggctggatga acttgtggaa
      601 cagcatgacg agcacggcgt agatcgccag cacgccgatc gccatggcga gcgcaaactc
      661 gccgaagatc tcccttatcc agcgtgagtc gcccgtcggc acgaggcgca cgccgtcggg
      721 caagttgcgc atggccggca gcgcctcgat cgcgtcgagc atgtcgccga gcgcctggcc
      781 gttcaagttc acgttcagcg tgatcttgcg gctgcgatcg acgcgcgtga tctgcgcagg
      841 tcccgcgccg agcgacacgt cggcgacgtt cgcgagcggc acgggcccgt tgcggcccgg
      901 cacggacagg ttctcgaggc gctccaggtc gctgcgcgac ttgtcgttca aacgcacgag
      961 gatcgggatc tggcggttgt cgaggttgaa cttcgagagg ctcgtgttga cgtcgccgct
     1021 cgtcgcgatg cgcgtcacga ggctcagcgt ctcggtcgtc actccgagct cggccgcgcg
     1081 ctcgggatcc ggccggatca cgatctcggg cttctgcagc gcggcgctgc tcgtgatcat
     1141 gccgaggccg ggcaacgagc gaatctcgcg ctcgacgttc gtggcggcca ggttcagccg
     1201 gttcggctcg tcgccgacga gcgtgacctc gagcgagctg ccgccgctgc cttggaactg
     1261 gaaacgcgca ccgggaatgt cgcgcagcag ctccgtggct ttgctctcga agatctgctg
     1321 agctccgcgc gtgccttcgg ggttgtccag ttggatcacc atcgtgccgc ggcgcacgtt
     1381 gccggcggag ccgccgccgg tcgggttgcg gcctccgccg ccgccctgcg cgccgaccgt
     1441 cgtgtagacg ctgcggacct cgggcagttg accgaggcgc ttgcgcgcgg cttcggccac
     1501 ggccagcgtg tcttcgagcg acgaccccgg cgcgagctcg acgctcaacg tcgtgaagcc
     1561 gttgctgccg gccggcgaga aagccgtcga cacgaacggt gccaggccga gcatcgcgcc
     1621 gatcgcgacg ctcgcgacgc cgagcgtcgt ccaccgatgt gcgaggcacc agcggacgcg
     1681 ctcgagatac caggtcatga tgcgcgccgg gcgctcgggc cggtcgtgcg gcttcaggaa
     1741 ataggcggcc atcatcggcg tcagcgtccg cgcgacgagc cgcgagcaga gcacggccac
     1801 cacgaccgtg aagctgaacg ggccgaagaa ctcgccggcg atgccgctca tgaacgcgac
     1861 ggggatgaag acggcgcaga tcgtcagcgt cgtcgcgacg acggcgaggc cgatctcgat
     1921 ggccgcgtcc atcgccgctt ccctcggctt cttgcccatg cgcaggtggc gcacgatgtt
     1981 ctcgacctcg acgatcgcgt cgtcgaccag gatgccgacg acgagcgaca gcgcgagcag
     2041 cgtcagaatg ttcagcgtgt agccgagcag ccagatcgcc cagaaggtgg ggatgatcga
     2101 cagcggcagg gcgacggccg acacggccgt cgcgcgccaa tcgcgcagga acaggaagac
     2161 gacgatgatc gcgaggaccg cgccttcggc gagcatctcc atcgaagcgt cgaacgactc
     2221 ctggatgtag tcgaccgtcg agctcgcttc ggcgaaatgg acgttcggat actccttctg
     2281 caaccgttcg acgacgacac gcgcctcctt cgcgacgtcg agcgcgctcg cgccccacga
     2341 gcgcacgatc tcgaagccga tgacgggctt gccgtcgagc agcgcggcgg cgcgttgctc
     2401 gccggcctgg tcgcgtacct cggcgatcgt gtcgagccgc acgctgcggc cgtcggggag
     2461 cgaaatcggc aaaccgcgca gatcgtagac gttgtcgatg atgcccgtcg tgcgcacggt
     2521 ctgctcgagg ccgcccaagc gcccttcccc gccgggatac tcggcctgaa tgcggcgcag
     2581 ctgccgcgag acgtcgctcg cggtggcgcc gagcgcggcc atgcggtccg gatcgagatc
     2641 gacgcggatt tcccgcgcga cgccgcccac gcgcgtcacg cgacccatgc cgggtacggc
     2701 cgaaagctcg cgcgtgacgg tgagatcgac gaaccacgac agctcggtgt cc
//
LOCUS       DASSNN010000632         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1620362, whole genome shotgun sequence.
ACCESSION   DASSNN010000632 DASSNN010000000
VERSION     DASSNN010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620362"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2474)
                     /locus_tag="VFL84_11810"
     CDS             complement(<1..>2474)
                     /locus_tag="VFL84_11810"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7609355.1"
                     /translation="TGALTLACATNVDTLPPPPGIWPIETLLDGTSARALFDLPEGVD
                     WPRGPWHRPPSRAIAMAIPQQGQARPAAIFIAGLNPFRPVDEAYDSFIALYVGQLAAA
                     LANAQAYAAERRRAEALAQIDRAKTTFFSNVSHELRTPLTLMLAPVADALASERPALT
                     RETLELVYRNGARLRKLVNALLEFSRLEAGRVQARFEPLALGAYTADLASTFASTIER
                     AGLRYVVEVPADEEPTYVDRDMWEKIVLNLISNAVKFTLEGEISVSLSRQDHRMVLVV
                     RDTGTGIPPSELPRIFDRFHRVEGSHGRTQEGTGIGLALVQELVRLHGGSIEVASTPG
                     KGTTFTVAIPVGAGHLPVERLTQARPAPLRTAHGTYVAEAERWLPDAGELEPLHEQAS
                     GPMRFAHILLADDNADMRAYLGRLLGARWRVSTAADGEQALELARREHPDLVVTDVMM
                     PRLDGFGLLAALRADPATSDIPVVMLSARAGEESRVEGFEAGADDYLVKPFTAREVVA
                     RVEAQLLRARIRSVEDEHRRRLASVFSHAPVAIAVLRGPHHVFEFANAPYLELIDNRD
                     VDGKPIRDALPDLEGQGIFELLDTVYRTGERYIGRSYRVVLPQQTGDARESFFDFVYQ
                     PTFDAQGRVDGIAVVAFDVTALARAHEAAEGANRAKDEFLAMLGHELRNPLAPILTAL
                     QLLRLRGVQAGERERTIIERQVRHLVGLVDDLLDISRITRGKIRLSPAPIEVSDAVAK
                     GIELASPLLEQHQHDLIVEVPTEGLGVLADPDRLAQVVSNLLTNAAKYTPPGGLIRVS
                     ATLEEDEVCLRVKDSGIGIDSTMLPRL"
BASE COUNT          380 a          864 c          842 g          388 t
ORIGIN      
        1 agccggggca gcatcgtgga gtcgatgccg atgccgctgt ctttgacccg aaggcagacc
       61 tcgtcctcct cgagcgttgc cgacacgcga atcagtccgc cgggcggggt gtatttcgcg
      121 gcgttcgtca gcagattgct gacgacttgc gccagccgat cggggtcggc gaggacgccg
      181 agcccctcgg tcgggacctc aacgatcaga tcgtgctggt gctgctccag cagggggctc
      241 gccagctcga tgcccttggc gacggcgtca ctcacttcaa tcggcgcggg gctcaggcgg
      301 atcttgccgc gcgtgatgcg tgagatgtcg agcaagtcgt ccacgaggcc gacgagatgc
      361 cgcacctgcc gctcgatgat cgtgcgctcg cgttcgcccg cctgaacgcc ccgcagccgc
      421 agtaactgca gcgcggtgag gatcggcgca agagggtttc gcagctcgtg gccgagcatc
      481 gcgaggaatt cgtccttcgc gcggttggcg ccctcggcgg cttcgtgcgc gcgcgccagc
      541 gccgtgacgt cgaaggcgac gaccgcgata ccgtccacgc gcccctgcgc gtcgaacgtc
      601 ggctgataca cgaaatcgaa gaagctctcg cgcgcgtcac ccgtctgctg aggcaggacg
      661 acgcggtacg agcggccgat atagcgctcg ccggtgcggt aaaccgtgtc gagcagctcg
      721 aagatgccct gcccctccag gtccggcagc gcgtcgcgga tcggcttgcc gtccacatcg
      781 cggttgtcga tcagctcgag atacggcgcg ttggcgaact cgaacacgtg atgcggcccg
      841 cgcaggacgg caatcgctac aggcgcgtgc gagaacaccg acgccagccg tcggcggtgc
      901 tcgtcctcga ccgatcggat gcgcgcgcgc agcagctgcg cctcgacgcg cgcgacgacc
      961 tcgcgagcgg tgaacggctt caccaggtag tcgtctgccc ctgcctcgaa cccctcgacg
     1021 cgcgactcct ctcccgcgcg cgccgacagc atgacgaccg ggatgtcgct cgtcgccggg
     1081 tccgcccgca aggcggcaag caggccgaag ccgtcgagcc tcggcatcat gacgtccgtc
     1141 acgaccagat cggggtgctc gcgtctcgca agctccagcg cctgttcgcc gtcggccgcg
     1201 gtgctcacgc gccaccgcgc gccgagcagt ctgccgaggt acgcgcgcat gtccgcgttg
     1261 tcgtcggcca gcaggatatg ggcgaaccgc atcggtccgg acgcctgctc gtgcagcggc
     1321 tccagctcgc ccgcgtccgg tagccagcgt tccgcctccg cgacatacgt gccgtgcgcg
     1381 gtgcgcaacg gcgccggacg cgcttgtgtg aggcgctcca ccggaagatg gcccgcgcca
     1441 accggaatcg cgacggtgaa cgtcgtcccc ttgcctggcg tgctggcgac ctcgatcgag
     1501 cccccgtgga gcctgaccag ttcctgcacg agcgccagcc cgatgccggt gccctcctgc
     1561 gtccgcccgt gggagccttc gacgcgatgg aagcggtcga agatcctcgg cagctccgac
     1621 ggtggaatgc ccgttcccgt gtcccgcacc acgagaacca tgcgatggtc ctgccgcgag
     1681 agggatacgc tgatttcacc ctccaacgtg aacttgaccg cgttcgagat gagattgagg
     1741 acaatctttt cccacatgtc gcgatcgaca taggtgggct cttcgtcggc cggcacctcg
     1801 accacgtagc ggagcccggc acgctcgatc gtggacgcga acgtgctcgc gagatccgcg
     1861 gtgtaggcgc cgagcgccag cggctcgaag cgcgcctgaa cgcggccggc ctcaagccgc
     1921 gagaactcga gcagcgcgtt cacgagtttt cgcagccgcg cgccgttgcg atagaccaac
     1981 tcgagcgtct cgcgggtgag cgccggccgt tcgctcgcaa gcgcgtcggc caccggcgca
     2041 agcatgagcg tcaacggcgt tcgcagctca tgactgacgt tcgaaaagaa cgtcgtcttc
     2101 gcgcggtcga tctgcgcgag cgcttcggcg cgtcggcgct ccgctgcata cgcctgcgcg
     2161 ttggcgagcg ctgcggcgag ctgcccgacg tagagcgcga tgaagctgtc gtaggcttcg
     2221 tccaccgggc gaaacggatt gaggccggcg atgaagatcg ccgccgggcg cgcctgcccc
     2281 tgctgcggga tcgccattgc gatcgcgcgc gagggcggcc gatgccacgg gccacgcggc
     2341 cagtccacgc cctcgggcag gtcgaacagc gccctggccg atgtcccgtc gagcagcgtc
     2401 tcgatcggcc agatgccggg cggcggcggc agcgtatcga cgtttgtcgc gcacgcgagc
     2461 gtcagcgccc cggt
//
LOCUS       DASSNN010000633         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_106789, whole genome shotgun sequence.
ACCESSION   DASSNN010000633 DASSNN010000000
VERSION     DASSNN010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106789"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..>2068)
                     /locus_tag="VFL84_11815"
     CDS             complement(114..>2068)
                     /locus_tag="VFL84_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7609356.1"
                     /translation="QHVEVRQSIIDASKYGPQFAGGSLSIPMNYESLRRAGATARAML
                     VAAAAKTWNVPASELTTQASTVRHAASNRSATYGQLAETAATLPVPDAASVTLKPKQS
                     FKLLGTRVTGVDNAKLVRGEPLFGIDQRVPGMLFAAFAKAPAIGARATAANLDKIKAM
                     RGVKNAFIVAHQGDPISFNPAAAATLSGVAIVADSTWNALQAKKALEVQWDTSQASND
                     SWTNAVAQAKSLAAKPAAQVLGEGGNVDAAFGAGKTVEAFYTYHYVAHSDLEPQNCTA
                     WFKGDSIEIWAPTQTPQNAVDAVAALLSLPKDKVTLHQLRGGGGFGRRLANDSVCEAA
                     LVSKQAGGVPVKVQWTREDDMAFDYYRPGGFHAQKASIDSAGKLTAYQNHFITFTMDG
                     KQPVSAATLDKTEFPANVLANNRIAQSMIPSKIPTGPWRAPGSNVIAFAIQSFLHECA
                     VAAKRDYVDFLVDVMGEPRATSSGPFGAGLHTGRAVNVIKAVAERSGWGRSLPKGRGL
                     GLAFHFSHQGHFAEVAEVSVDASKKVDVHKVWVVADIGPIVNLSSAENQCQGSVIDAL
                     STAMGLKITFEGGKVEQTNFHQYPIVRIDKAPEIDVHFLATDYAPTGCGEPAFPPAAP
                     AIANAIFASTGQRIRTLPFSVEGYSV"
BASE COUNT          283 a          704 c          715 g          366 t
ORIGIN      
        1 ccagccaggc ggcgcgctcg agcgccggct ggtctcgtcc ggccgccgtg tcgacgacca
       61 cgaggatggt gttctcccac atgagcaggc gcctgtaagt gcttgttccc tctctagacg
      121 gagtagccct ccacgctgaa gggcagcgtg cggatgcgct ggcccgtcga cgcgaagatc
      181 gcgttcgcga tcgccggcgc ggccggcggg aacgccggct cgccgcaacc ggtcggcgcg
      241 taatccgtcg caaggaagtg cacgtcgatc tccggggcct tgtcgatgcg cacgatcggg
      301 tactggtgga agttcgtctg ctcgaccttg ccgccctcga acgtgatctt gaggcccatc
      361 gccgtgctca gcgcgtcgat gacggaacct tggcactgat tctcggcgct gctcaagttc
      421 acgatcgggc cgatgtcggc gacgacccag accttgtgca cgtcgacctt cttgctcgcg
      481 tcgacgctga cctccgcgac ctcggcgaag tgcccttgat ggctgaagtg gaacgcgagg
      541 ccgagcccgc ggcccttcgg caacgagcgg ccccagcccg agcgctcggc gacggccttg
      601 atcacgttga cggcgcggcc cgtgtgcaac ccggcgccga acggaccaga cgaggtcgca
      661 cgcggctcgc ccatgacgtc gaccaagaag tcgacgtagt cgcgcttcgc ggcgacggcg
      721 cactcgtgca agaagctctg gatcgcgaac gcgatgacgt tgctgcccgg cgcgcgccac
      781 ggtcccgtcg gaatcttcga cgggatcatc gactgcgcga tgcggttgtt cgcgagcacg
      841 ttggccggga actcggtctt gtcgagcgtc gcggccgaga ccggctgctt gccgtccatc
      901 gtgaacgtga tgaagtggtt ctgatacgcg gtcagcttgc cggcgctgtc gatggaggcc
      961 ttctgcgcgt ggaagccgcc ggggcggtag tagtcgaacg ccatgtcgtc ctcgcgcgtc
     1021 cactgcacct tgaccggcac gccgcccgct tgcttcgaga cgagcgcggc ttcgcagacg
     1081 ctgtcgttcg cgagccggcg gccgaaaccg ccgccgccgc gcagctggtg cagcgtgact
     1141 ttgtccttcg gcaggctcag cagcgccgcg accgcgtcga cggcgttctg cggcgtctgc
     1201 gtcggcgccc agatctcgat gctgtcgccc ttgaaccagg ccgtgcagtt ctgcggctcg
     1261 aggtcggagt gcgcgacgta gtgatacgtg tagaacgcct cgactgtctt gccggcgccg
     1321 aacgccgcgt cgacgttgcc gccctcgccg agcacttgcg cggcgggctt cgccgcgagc
     1381 gacttggcct gcgcaacggc gttggtccag ctgtcgttcg acgcttgtga cgtgtcccac
     1441 tgaacctcga gcgccttctt cgcctggagc gcattccaag tggagtccgc gacgatcgcg
     1501 acgcccgaca gcgtggccgc ggccgcggga ttgaagctga tcggatcgcc ctggtgcgcg
     1561 acgatgaacg cgttcttgac gccgcgcatc gccttgatct tgtcgagatt cgccgccgtg
     1621 gcgcgcgcgc cgatcgccgg cgcttttgcg aacgcggcga acagcatgcc cgggacgcgc
     1681 tggtcgatgc cgaacagcgg ctcgccgcgc acgagcttcg cgttgtcgac gcccgtgacg
     1741 cgcgtgccga gcagcttgaa gctctgcttc ggcttcagcg tgacgctcgc cgcatcgggc
     1801 accggcaacg tcgcggcggt ttcggcgagc tggccgtagg tcgcgctgcg gttgctggcc
     1861 gcgtggcgca ccgtgctcgc ctgcgtcgtg agctcggagg cgggcacgtt ccaggtcttc
     1921 gccgcggcgg cgacgagcat cgcgcgcgcc gtcgcgcctg cgcggcgcag cgactcgtag
     1981 ttcatcggga tcgacagcga gccgcccgcg aattgcgggc cgtacttgct cgcgtcgatg
     2041 atcgattggc gcacctcgac gtgctgcc
//
LOCUS       DASSNN010000634         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1321915, whole genome shotgun sequence.
ACCESSION   DASSNN010000634 DASSNN010000000
VERSION     DASSNN010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321915"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFL84_11820"
     CDS             <1..783
                     /locus_tag="VFL84_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609357.1"
                     /translation="SASAQQSGAAEAGQRTARAGESTKDAKPSAPTPRRADGTVDLGG
                     DGIWDQPWITDFGKQLVGDQQIPFLPWTKAMYDYNKSNQVAYDPQGFCLPPGGPRMFG
                     TPYPSQFIQDRNRIVVIFEGGAHVWREIHMDGRPLPKREDLNPTYFGYSVGRWEGDTL
                     VVETTGFNEKTWLNFNGTMHTDELYTVERISRPNRDTLHYEATITDPGAYTKPWTVAW
                     DIPWLEGAELAEYICQENNKFLLELKDDNGNPFFEDIPTETP"
     gene            805..2043
                     /locus_tag="VFL84_11825"
     CDS             805..2043
                     /locus_tag="VFL84_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET7609358.1"
                     /translation="MNLRPARWFALLCFALPFLGGCGAGQPGESGAHWVATWGASPFG
                     FVAFGPAPAPVPFMNQTVRQKLRISVGGEQVRVRFSNELGTTPLVIGAATVALAGRDA
                     AVDGASLRKLTFGGADSIKIPPGAPALSDPVDLKLADLAEIAVSIYLPDETPPATMHM
                     GRTAYVSGAGDFTQAADLAGAELTANHVFLTGVYVSTNENVAVVAALGDSITDGAAST
                     PHSYNSWPDHFAPRIAAANVAVVNQGISGNQVLRDGAGVSALARFDRDVLSTPGLTHV
                     IVLEGINDIGTGGFNFPGTTGPAPPERTADELIAGYRQLIARAHARGLKIYGGTLTPF
                     EGTFAGYYTPAKDEVRLAVNEWIRGSGEFDAVIDFEAAIRDEANPRVMRAEYDSGDKL
                     HPGDAGYKRMADSIDAALFR"
     gene            complement(2051..2578)
                     /locus_tag="VFL84_11830"
     CDS             complement(2051..2578)
                     /locus_tag="VFL84_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609359.1"
                     /translation="MQSPEGDAAEESADSASAATAPIMTVNQLMRGILFPLGNTVFYA
                     QADDPAAVPRDPMPSASTNPLTGIFGGWQAVENSALALVESAELLKVPGRMCSNGEVV
                     DVANADWIRFVDDYRQKSLAAYEAVLTKDPDAIVLASGDLSEACLACHRVYRRELTQG
                     DPSQRCIPGPAQTRG"
BASE COUNT          501 a          970 c          944 g          455 t
ORIGIN      
        1 tcggcgtccg cgcagcaaag cggcgcggcc gaggcgggtc agcgcacggc gcgagcggga
       61 gagagcacga aggacgcgaa gccttcggca ccgacgccgc ggcgcgccga cgggaccgtc
      121 gatctcggcg gggacggcat ctgggatcaa ccgtggatca cggatttcgg caagcagctc
      181 gtgggcgacc agcagatccc gttcttgccc tggacgaagg cgatgtacga ctacaacaag
      241 tcgaaccagg tcgcctatga cccgcagggc ttctgcctgc cgccgggcgg cccgcgcatg
      301 ttcggcacgc cgtatccgtc gcaattcatc caagaccgaa accgcatcgt cgtgatcttc
      361 gagggcggcg cgcacgtctg gcgcgagatc cacatggatg gacgcccgct gccaaagcgc
      421 gaagacctga acccgacgta cttcggttat tccgtcggcc gctgggaagg cgacacgctg
      481 gtcgtcgaga cgaccggctt caacgagaag acatggctga acttcaacgg cacgatgcat
      541 acggacgagc tctacaccgt cgagcgcatc agccggccga atcgcgacac gttgcactac
      601 gaggcgacga tcacggaccc cggcgcctac acgaagccgt ggacggtcgc ctgggacatt
      661 ccgtggctcg agggagccga gctcgcggag tacatctgcc aagagaacaa caagttcttg
      721 ctcgagctca aggacgacaa cggcaatccg ttcttcgagg acatcccgac cgaaacgccc
      781 tgagcttagg cgaggagagc gcgcatgaac ttgaggcccg ctcgttggtt cgcgctgctt
      841 tgcttcgcgc tcccattcct gggcggttgc ggagccgggc aacccggcga gtcaggcgcg
      901 cactgggtcg ccacgtgggg tgcgagcccg ttcggtttcg tcgcgttcgg gcccgcgccg
      961 gccccggtgc cgttcatgaa ccagaccgtg cggcagaagc tgcgcatcag cgtcggcggc
     1021 gagcaagtgc gcgtccgctt ctcgaacgag ctcggcacga cgccgcttgt gatcggcgcg
     1081 gccacggtcg cgctcgccgg ccgcgatgcc gcggtcgatg gcgcgagcct gcgcaagctg
     1141 acgttcggcg gcgcggattc gatcaagatt ccgcccggcg cgcccgcgct cagcgacccg
     1201 gtcgatctta agctcgcgga tctcgccgag atcgcggtca gtatctacct gcccgacgaa
     1261 actccgcccg cgacgatgca catgggccgg accgcatacg tttccggcgc cggcgatttc
     1321 acgcaagccg ccgaccttgc gggcgccgag ctgaccgcga accacgtctt tctgacgggt
     1381 gtctacgtga gcacgaacga aaacgtcgcg gtcgtcgcgg cgctcggcga ctcgatcacc
     1441 gacggcgccg cgtcgacgcc gcactcctac aacagttggc ccgatcattt cgcgccacgc
     1501 atcgccgccg ccaatgtcgc cgtcgtgaat cagggcatct cgggcaatca ggtgctccgc
     1561 gatggcgcgg gcgtcagcgc gctggcgcgg ttcgatcgcg acgtgctgtc gacgccgggc
     1621 ttgacgcacg tcatcgttct cgaaggcatc aacgacatcg gcaccggtgg cttcaacttc
     1681 ccgggcacca cgggccccgc gccgcccgag cgcacggccg acgagctgat cgcgggctac
     1741 cgccaactga tcgcacgcgc gcacgcgagg ggcctgaaga tctacggcgg gacgctgacg
     1801 ccgttcgaag ggactttcgc gggctattac acgcccgcga aagacgaagt gcgcctcgcg
     1861 gtcaacgagt ggatccgcgg cagcggcgag ttcgatgccg tcatcgactt cgaagcggcg
     1921 atccgggacg aagcgaaccc gcgcgtgatg cgcgccgagt acgattcggg cgacaagctg
     1981 caccccggcg atgccggtta caaacggatg gccgactcga tcgacgcggc gctattccgc
     2041 tgagcgcccg ttagccgcgg gtttgcgccg ggcccggaat gcaacgctgg ctcgggtcgc
     2101 cttgcgtcag ctcgcgccgg tacacgcggt ggcacgccaa gcacgcttct gaaaggtcgc
     2161 cgctcgcgag cacgatcgcg tccggatctt tcgtgagcac ggcctcgtaa gccgccaagc
     2221 tcttctgccg gtagtcgtcg acgaaccgga tccagtcggc gttcgcgacg tcgacgactt
     2281 cgccgttcga gcacatgcgg cccggcacct tcagtagctc cgcggactcc acgagcgcga
     2341 gcgcgctgtt ctcgacggct tgccaaccgc cgaagattcc ggtcaaggga ttcgttgaag
     2401 ccgagggcat gggatcgcgc ggcaccgcgg cggggtcgtc ggcctgcgcg tagaacaccg
     2461 tgttgccgag cgggaacaag atgccgcgca tgagctggtt gacggtcatg atcggcgcag
     2521 tcgccgcgct agccgagtcc gcgctctcct cggcggcatc gccttcgggc gactgcacgc
     2581 aagcgctcgc cgaagcggcg aaacacgaaa tggccaatct ccaccatcga ctacggttcg
     2641 acatgcaaac acccccgcaa ctgatgcgcg tgagcttagc gcatcgcgcg tcggaaccgt
     2701 tctgacatac agtaggtctc gtgagcacgc ttctccagga tctgcgctac gccgctcgat
     2761 ggctctcccg caatcgcgga ttcacggccg tcgctgtcct cacgctcgcg ctcggcgttg
     2821 gggcgaacac cgcgatcttc agcgtgctgc aggcgattgt gttgcgagac
//
LOCUS       DASSNN010000635         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_341958, whole genome shotgun sequence.
ACCESSION   DASSNN010000635 DASSNN010000000
VERSION     DASSNN010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341958"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1399)
                     /gene="pabB"
                     /locus_tag="VFL84_11835"
     CDS             complement(26..1399)
                     /gene="pabB"
                     /locus_tag="VFL84_11835"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET7609360.1"
                     /translation="MATPRITELTHSTSAARRFRTLAGLPWCMWLDSAAADDHERFDV
                     LVADPYVTLRTRGAVTEIARRGAPARESQRPPFELVREQLGDVAAASPGLPFCGGAVG
                     YFGYDLGRRFERIPAIAAADIAMPDLAIGIYDWAVVVDHAARRTWLVGNGRDARTFAE
                     WPRLVARLSAEPPPAPAPFRVRTRPASNLDRGAYAGAFRAVQEHIRRGDCYQVNLTQR
                     FEAEAEGDAWHAYLRLREINPAPFAAYLDLPDGKVVCSSPERFLRLRDGRVETKPIKG
                     TRPRSKDPARDRALADELRASAKDRAENVMIVDLLRNDLGKCCVPGSVHASKLFEIES
                     FASVHQLVSTVEGRLAPGLHALDLVAACFPGGSITGAPKVAAMKIIEELEPQRRSVYC
                     GSIGYIGFDGNMDTNIAIRTLVQQGSHVYTWAGGGVVADSNVDAEYQESLDKAAAMLA
                     VMDPAAD"
     gene            1575..2345
                     /locus_tag="VFL84_11840"
     CDS             1575..2345
                     /locus_tag="VFL84_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7609361.1"
                     /translation="MTQFTRRKLKINGIETVVLEAGSGSPLVFLHGAGTVTGFDFAAS
                     WSRKFRVVIPYHPGFGPSADDPAVTEIHDFVLHYLELFDALELRQFRLVGQSMGGFIA
                     TKFAIEHGARIDKLVLVCPIGLPVPPGQPTVNFLEVPPEQLPTLLGHDPQTVIKHLPA
                     GAPPPDFIAERVKEAVTAGRVLGGGTRTFDEKLPRYLHRLTMPTLLIWGNQDKLTPTA
                     QHKTWAKALPNATVRLFDNAGHLVLDEAPGAADAVAAF"
BASE COUNT          372 a          832 c          820 g          377 t
ORIGIN      
        1 tgcgcggcgg cggctcgggc gcggtctagt cggcggccgg atccatgacg gccagcatcg
       61 ccgccgcctt gtcgaggctt tcctgatact cggcgtcgac gttcgaatcc gcgacgacgc
      121 cgcccccggc ccacgtgtac acgtggctgc cctgctgcac gagcgtgcgg atcgcgatgt
      181 tcgtgtccat gttgccgtcg aagccgatgt agccgatcga gccgcagtag acgctgcgcc
      241 gctgcggctc gagctcctcg atgatcttca tcgccgcgac tttcggcgca ccggtgatcg
      301 agccgccggg gaagcacgcc gcgacgagat cgagcgcgtg cagccccggc gcgagccgcc
      361 cttcgacggt gctcacgagc tgatgcacgc tcgcgaacga ctcgatctcg aacagcttgc
      421 tcgcgtgcac ggagcccggc acgcagcact tgccgaggtc gttgcgcaga aggtccacga
      481 tcatgacgtt ctcggcgcgg tccttcgcgc tcgcgcgcag ctcgtcggcg agcgcgcggt
      541 cgcgcgccgg atccttggag cgcggccgcg tgcctttgat cggcttcgtc tcgacgcgcc
      601 cgtctcgaag acgcaagaac cgctcgggcg acgagcagac gactttgccg tcgggcaggt
      661 cgagatacgc cgcgaacggc gccgggttga tctcgcgcag gcgcaggtag gcgtgccacg
      721 cgtcgccctc tgcttcggcc tcgaaccgct gcgtcagatt cacttgatag cagtcgccac
      781 ggcggatgtg ctcttgcacg gcgcgaaacg cgcccgcgta tgctccgcgg tcgaggttcg
      841 acgcgggccg cgtgcgcacg cgaaacggcg cgggcgccgg cggcggctcg gccgagagac
      901 gcgcgacgag gcgcggccac tccgcgaacg tccgcgcgtc gcggccgttg ccgacgagcc
      961 aggtgcgccg cgcggcgtgg tcgacgacga cggcccagtc gtagatgccg atcgcgagat
     1021 cgggcatcgc gatatcggcc gccgcgatcg ccggaatgcg ctcgaatctg cggccgaggt
     1081 cgtagccgaa atagccgacc gctccgccgc aaaacggcag gccgggagaa gcggccgcga
     1141 cgtcgccgag ctgctcgcgc acgagctcga agggcggacg ctgcgactcg cgagccggcg
     1201 cgccgcgcct cgcaatctcg gtgacagcgc cgcgtgtgcg cagcgtgacg tacggatcgg
     1261 caacgaggac gtcgaagcgc tcgtggtcgt cggccgccgc gctgtcgagc cacatgcacc
     1321 acggcaagcc cgcgagcgtg cgaaagcgcc gcgccgcgga cgttgagtgc gtgagctcgg
     1381 tgattcgcgg cgttgccaaa cctgatcctc tccggaggct cgcgaccgcg gccctcggcc
     1441 gcgccggcgg cggggcgcca attttcgagc aaggctacgc gcgcgacaat atgcaggcgc
     1501 cgttcgcgcg ttgcatcgcc cggtccaaac acatagtgtt cgacttctcg cgcgctcggc
     1561 ggaggaccgc aacgatgacg caattcactc gccgcaagct gaagatcaat ggcatcgaga
     1621 ccgtggttct cgaagcgggc agcggatcgc cgctcgtgtt cttgcacggc gccggaaccg
     1681 tcacgggctt cgatttcgcg gcgtcctgga gccggaaatt tcgcgtcgtc attccttatc
     1741 atcccggctt tgggccgtcg gccgacgatc ccgccgtgac ggagatccac gatttcgtgc
     1801 tccactactt ggagctgttc gacgcgctcg agctgcgtca atttcgtctc gtgggccagt
     1861 cgatgggcgg cttcatcgcg actaagttcg cgatcgagca cggcgccaga atcgacaagc
     1921 tcgtgctcgt ctgccccatc ggcctgccgg tgccgcccgg gcagccgacc gtgaacttcc
     1981 tcgaggtgcc acccgagcag ttgcccacgt tactcgggca cgacccgcag accgtgatca
     2041 agcacttgcc ggccggcgcg ccgccgcccg acttcatcgc cgagcgcgtc aaggaagcgg
     2101 tgacggccgg gcgcgttctc ggcggcggca cacgcacgtt cgacgagaag ctgccgcgct
     2161 atttgcaccg gctgacgatg ccgacgctcc taatctgggg caatcaagac aagctgaccc
     2221 cgacggcgca gcacaagacg tgggcgaaag cgttgccgaa cgcgacggtg cgcttgttcg
     2281 acaacgccgg gcatctcgtg ctcgacgagg cgcccggcgc agccgacgcc gtcgcggcgt
     2341 tctagaggcc gagctgctcg ttcgcccagg gactgacgta acgccaattc gggccgtccg
     2401 t
//
LOCUS       DASSNN010000636         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1238651, whole genome shotgun sequence.
ACCESSION   DASSNN010000636 DASSNN010000000
VERSION     DASSNN010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238651"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..578)
                     /locus_tag="VFL84_11845"
     CDS             complement(24..578)
                     /locus_tag="VFL84_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HET7609362.1"
                     /translation="MEIYVSTDVEADGPVPGTNSMLSFASAAYTAEGKLLGTFSANLE
                     TLPGATANPKTAEWWLTQPEAWAACRRDPQPPEVAMREYAAWVGALPGKPVFVAYPAG
                     FDFVFVYWYLMRFVGQSPFGFSAIDIKSYAMALLKTEYRHATKSRLPAHWFGTQAHTH
                     IALDDAIEQGTLFCNMLAENLGRP"
     gene            complement(600..1715)
                     /locus_tag="VFL84_11850"
     CDS             complement(600..1715)
                     /locus_tag="VFL84_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609363.1"
                     /translation="MSKKRPKTSTAARRSIFPPPPRGMKGRGAELATLARTVLATRPT
                     RIALVGPGGSGKSMLATALGHKLRAAFGGRVDWFRSASWGFYTLSEMLALRFGTGRGD
                     GRVQRLQEFLARGPQRLIVLDNHENDAAVERLFETFADSNATFVITARRCLLAGVFVF
                     PVVAPLVTSGKSAFPRVAAVTRLLRWNPLALDIADGIVKSRAASAKSLGKFLQDAGVT
                     RVRTLEHEDDLPEIALLIAWAWPRLGEAARRILAVLAHVEGDHVDLVSLTELARVERI
                     GPALAELTRWHLVLEPVRERYTLHAVVRHAVGRRTQPDADRVFSHYVTLLEQHPERLE
                     LEQSHLFAAMEHASRKNDMYALLRIEALARRLSGEER"
     gene            1805..2215
                     /locus_tag="VFL84_11855"
     CDS             1805..2215
                     /locus_tag="VFL84_11855"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609364.1"
                     /translation="MKRAHWFALAAGVLSAAVTAGVSAHHSFAAVYDMQKPITVKGTI
                     VNVRLANPHSWFYLDVKNDAGQVERWAFEAGTPSGMIRNGYKPNIVPPGSEVTITGFR
                     GRVESEHIGMLRQLTTADGTVYGLFGPQEGPNAR"
     gene            2255..>2731
                     /locus_tag="VFL84_11860"
     CDS             2255..>2731
                     /locus_tag="VFL84_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609365.1"
                     /translation="MSRALEGSLAVPLAAAMLLTLGGANVAVAQDQAPAKPEAVPRMA
                     DGHPDLSGVWWGGADVGGRRPGGGGGGPPGGRPGGAGGPGGAAPTPPPSFTSLYQPWA
                     AERAKTLSDQDDPTLHCVPVAFGTLNVRLWNVGAVGQIIATPKMVVLLSETFHDYQL"
BASE COUNT          446 a          905 c          949 g          431 t
ORIGIN      
        1 gcgccaagac gcgcttcggc tgactacggg cggccgagat tctcggcgag catgttgcag
       61 aacagcgtgc cttgctcgat cgcgtcgtcg agcgcgatgt gcgtgtgagc ttgcgtcccg
      121 aaccaatgcg cgggcaaccg cgacttcgtg gcgtgccggt actcggtctt gagcagcgcc
      181 atcgcgtagc tcttgatgtc gatcgccgag aatccgaacg ggctctgacc gacgaagcgc
      241 atcagatacc agtagacgaa cacgaaatcg aatccggcgg gataggcgac gaacaccggc
      301 tttccgggca gcgcgccgac ccaagcggcg tactcgcgca tggcgacttc cggaggctgc
      361 gggtcgcgac gacacgcggc ccacgcttcc ggctgcgtga gccaccactc ggcggttttc
      421 ggattcgcag tcgcgcccgg cagcgtttca aggttcgcgc tgaacgtgcc cagtagcttg
      481 ccttcggccg tgtaggcggc cgatgcgaag ctcagcatgg agttcgtccc gggaaccggt
      541 ccgtcggcct cgacatccgt gcttacgtag atctccatgc tgctcgcgcg cccctgtgtt
      601 cagcgctcct cgccgctgag cctgcgcgcg agcgcttcga ttcgcaacag cgcatacatg
      661 tcgttcttgc gcgaggcgtg ctccatcgcc gcgaacagat gcgactgctc gagctcgagc
      721 cgctccggat gctgctcgag cagcgtgacg tagtgcgaga agacacgatc ggcgtcgggc
      781 tgcgtgcgcc gaccaacggc atgacgaacg accgcgtgca gcgtgtagcg ctcgcgcacc
      841 ggctcgagca cgaggtgcca gcgcgtaagc tccgcgagcg cggggccgat acgttccacg
      901 cgcgcaagct cggtaagcga caccaagtcg acatgatccc cttcgacgtg cgcgagcact
      961 gccaggatgc ggcgcgccgc ctcgccgagg cgcggccagg cccaagcgat cagcagcgcg
     1021 atctcgggca agtcgtcctc gtgctcgagc gtgcgcactc tcgtcacgcc ggcgtcttgc
     1081 aagaacttgc cgagcgattt cgcgctcgcg gctcgcgact tcacgatgcc gtcggcgatg
     1141 tcgagcgcga gcgggttcca ccgcaacaga cgcgtgaccg cggccacgcg cggaaacgcg
     1201 ctcttgccgg aagtgacgag cggcgcgacg accgggaaga cgaacactcc cgcgagcaga
     1261 cagcggcgcg ccgtgatgac gaacgttgcg ttcgagtcgg cgaacgtctc gaacaagcgc
     1321 tcgacggcgg catcgttctc gtggttgtcg agcacgatga gccgttgcgg cccgcgcgcc
     1381 aggaattcct gcaagcgctg gacgcggccg tcgccgcgcc ccgttccgaa gcgcagcgcg
     1441 agcatttcgc tcaacgtgta gaagccccag ctcgccgagc gaaaccagtc gacgcgcccg
     1501 ccgaatgccg cgcgcagctt gtgccccagc gccgtggcca gcatcgattt gccgctgccg
     1561 ccgggcccga cgagcgcgat gcgcgtcggt cgcgtggcga gcacggtgcg cgcgagcgtg
     1621 gcgagctcgg cgccgcggcc tttcatgccg cggggcggcg gcggaaagat cgagcggcgg
     1681 gccgcggtgc tggttttggg ccttttcttc gacattcttg tggtgcctcg gcagctgcac
     1741 tttacatggc ggctcgcatt ggatgcgccg agcgtggaga atgaacgcac ggaggaactc
     1801 agcaatgaaa cgagctcact ggttcgcgct cgcggcagga gtgctctcgg ccgccgtgac
     1861 ggcgggggtc tcggcgcatc actcgttcgc ggcggtctac gacatgcaga agccgatcac
     1921 ggtgaaaggc acgatcgtga acgtccggct cgcgaacccg cattcgtggt tctacctcga
     1981 cgtgaagaac gatgcgggcc aagtcgagcg ctgggcgttc gaggccggca cgccaagcgg
     2041 catgatccgc aacggctaca agccgaacat cgtcccgccc ggctccgagg tcacgatcac
     2101 cggtttcaga gggcgcgtcg agtccgagca catcggcatg ctgcggcagc tcacgacggc
     2161 cgacggcacc gtgtacggtt tgttcgggcc gcaagaagga cccaatgcac ggtgacgcgc
     2221 gcttgcgtgc cgctgtgaac ggaggactgc gatcatgagt cgtgctctcg aaggatccct
     2281 cgcggtgccg ttggcggccg cgatgctgct cacgctcggc ggcgcgaacg ttgccgtcgc
     2341 gcaagaccaa gcgccggcga aacccgaggc cgtgccgcgc atggcggacg gccaccccga
     2401 tctctccgga gtctggtggg gcggcgccga tgtcggcggg cggcgtccgg gcggtggcgg
     2461 tgggggcccg ccgggcggac ggccgggcgg agccggtggg ccgggcggcg cggcgccgac
     2521 gccgccaccg tcgttcacga gcctctatca accctgggcg gccgagcgtg cgaagacgct
     2581 cagcgaccaa gacgatccga cgctgcactg cgtgcccgtc gcgttcggaa ccctgaacgt
     2641 gcggctctgg aacgtcggcg cggtcgggca gatcatcgcg acgccgaaga tggtcgtgct
     2701 cttgtccgag acgtttcacg attatcagct c
//
LOCUS       DASSNN010000637         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_342032, whole genome shotgun sequence.
ACCESSION   DASSNN010000637 DASSNN010000000
VERSION     DASSNN010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342032"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VFL84_11865"
     CDS             <1..843
                     /locus_tag="VFL84_11865"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7609366.1"
                     /translation="LHRRGRAYQHRFGQRAQRFAIELFRQAIALDRNYAPAWAALALS
                     YVLLYRYSLASEANRNEALAAARRAVELDSTLAEAWVAKGAATTICCDYGTAEQALAK
                     AVSLGPASFEAHYYYGRAATEMGEFAKAAALYERAAAIEPDDYQSLVFAAQCYRSLGQ
                     GARGGDAERRALAAAERALARDPTDARALVLSASALIHAGRAAEARSWVERACALEPD
                     EPHCRYNAACAYVLLGEHERALDMLEALDLKNMANRAWMQHDIEFDPLREHPRFKAIM
                     AVTR"
     gene            964..1320
                     /locus_tag="VFL84_11870"
     CDS             964..1320
                     /locus_tag="VFL84_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HET7609367.1"
                     /translation="MSSFRKTSVLAVIAALGALSTFTSAAQGTTPSAPARSVAVSYGH
                     LDLSDTRSIDALYRRLASAAERACGSYDGRDLRERSDWRRCRDEAFSTAIAHLVEMRI
                     AAVNHQRNRRATVPGG"
     gene            complement(1281..>2165)
                     /locus_tag="VFL84_11875"
     CDS             complement(1281..>2165)
                     /locus_tag="VFL84_11875"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HET7609368.1"
                     /translation="VPVERVRRDGAAAVIAADAVVTAEQFVHRRASDRAQGALCLRAG
                     TRLGAPEIAVLAGAGRASALVAELPRVAVMSTGDELVDVGKPIAPQQIRSTNDRAVEA
                     SLTQHRLAQVTRARLRDDANALAVAIDRLDTEHDMLVLSGGISMGKFDFVPSVLTELG
                     AKLVLKGVQQRPGRPMWFGVSARGKPIFALPGNPVSTLVCATRYVLPALRQASGLAPA
                     PPELVELTAPIEASPALTLFTPVTLSSSERATLLAAPHPTNTSGDFVTLAGTDGFVEL
                     AAKRGSHPPGTVARLFRW"
BASE COUNT          340 a          761 c          754 g          310 t
ORIGIN      
        1 ctgcaccgca gaggccgcgc ctaccagcac cgcttcggcc aacgcgcgca gcgcttcgcg
       61 atcgagctct tccggcaggc gattgcactc gaccgcaact acgcacctgc atgggccgcg
      121 ctcgcgctga gctacgtgct gctctatcgc tattcattgg cgagcgaagc gaaccgcaac
      181 gaagcgctcg ccgccgcgcg ccgcgcggtc gagctcgatt ccactttggc cgaggcctgg
      241 gttgcgaaag gggcggctac gacgatctgt tgcgactacg gtacggcgga gcaggcgctc
      301 gcgaaggccg tgagcctcgg gccggcgtcg ttcgaagcgc actactacta cggtcgcgct
      361 gccaccgaga tgggagaatt cgcgaaggca gccgcgctgt acgaacgcgc cgctgcgatc
      421 gagcccgacg actaccagtc gctcgtgttc gcggcccagt gctatcgctc gctcgggcaa
      481 ggcgcacgcg gcggcgacgc ggaacgacga gcgctggcgg ccgccgagcg cgccctcgcg
      541 cgcgacccta ccgacgcgcg ggcactggtc ttgagcgcat cggcgctgat tcacgccggg
      601 cgggccgccg aagcgcgctc gtgggtggag cgcgcctgcg cgctcgagcc ggacgagccg
      661 cactgccgtt acaacgctgc ctgcgcctac gtgctgctcg gcgagcacga gcgcgcgctc
      721 gacatgctcg aggcgctcga cctcaagaac atggccaatc gcgcctggat gcagcacgac
      781 atcgagttcg atccgctccg cgagcatccg cgcttcaaag ccatcatggc ggttacgcgc
      841 tgacgtaagc tcggtcacca aaaagtcagc caccgatcac ggggcggtca agagatcgcg
      901 gcgctgctcc ctcatcctgt gcgccatgcc ggctaccggc acaacccaaa caggagatcg
      961 gacatgagca gctttcgcaa gacatcggtc ctcgcggtga tcgccgcgtt gggcgctttg
     1021 tcgaccttta cttcggccgc acagggtacg acgccgtccg cgccggcacg ctcggtagcg
     1081 gtgagctacg gccacctcga cctgagcgac acgcgctcca tcgatgcctt gtaccggcgg
     1141 ctcgcctccg cagcggagcg tgcgtgcggc tcgtacgatg ggcgcgacct gcgcgagcgc
     1201 agcgactggc gccgttgccg cgacgaagcc ttcagcacgg cgattgcgca cctcgtcgag
     1261 atgcggatcg ccgcggtgaa tcaccagcgg aataggcgtg cgacggtgcc gggcggatga
     1321 gagccgcgct tcgcggcgag ctccacgaac ccgtcggtgc cggcgagcgt cacgaagtcg
     1381 cccgacgtgt tcgtggggtg cggcgcggca agcagcgttg cgcgttccga ggacgatagc
     1441 gtcaccggcg tgaaaagcgt cagcgccggc gacgcttcga tcggcgccgt gagctcgacg
     1501 agctcgggcg gcgcgggcgc gaggccggag gcctgccgca aggccggcag cacgtagcgc
     1561 gtcgcgcaga cgagcgtcga gacgggattg ccgggcagtg cgaagatcgg tttgccgcgc
     1621 gcgctgacac cgaaccacat cggccgaccg gggcgctgct gcacgccttt cagcacgagc
     1681 ttcgcgccga gctccgtgag cacggacggc acgaagtcga acttgcccat cgagatgccg
     1741 ccgctcaaga cgagcatgtc gtgctcggtg tcgagccgat cgatggcgac ggcgagcgcg
     1801 ttcgcgtcgt cgcgcagccg cgcgcgtgtc acctgcgcca agcggtgctg cgtgaggctc
     1861 gcctccacgg cgcggtcgtt cgtcgagcgg atctgttgcg gcgcgatcgg cttgcctacg
     1921 tcgacgagct cgtcgccggt cgacatcacc gcgacgcgcg gcaactccgc gacgagagcg
     1981 ctcgcccggc ccgcgccggc cagcacggcg atctcgggcg cgcccaagcg tgtgccggcg
     2041 cgcaggcaca aggccccttg agcccggtcg ctggcgcgcc tgtgaacgaa ttgctcggcc
     2101 gtcacgaccg cgtccgcggc gatgacagcc gcggcgccgt cgcgtcgcac gcgctcgacc
     2161 ggcac
//
LOCUS       DASSNN010000638         5464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1321978, whole genome shotgun sequence.
ACCESSION   DASSNN010000638 DASSNN010000000
VERSION     DASSNN010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321978"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1732)
                     /locus_tag="VFL84_11880"
     CDS             complement(38..1732)
                     /locus_tag="VFL84_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7609369.1"
                     /translation="MPAKPWLKSYSPGVPAEIDPDRYPSLVALLEASFARFSAQPAFT
                     NLGTTLSFREVERLSRAFAAYLQALGLERGDRVAIMLPNTLQSPVALFGILRAGMVAV
                     NVNPMYTITELGHQLADSGARAVVVLENFAHTVGAALPQTNVQTVIVTRLGDHCSPLK
                     RAVVNFVVKHVKKLVRPWQIPGAVAYRDALARGAALAYTAPRLGGGDLAFLQYTGGTT
                     GRAKGAMLTQRNMVANTLQAAAWARPFYTPDAGVVVTPLPLYHVYSLTANLFCFVELG
                     AQNLLITDPRDFKGFVALLRRHKFAFMTGVNTLFNALLHTDGFAKLDFSSLRVVMGGG
                     MAVQREVAARWQAVTGVVISQGYGLTEASPIVTGNPLHLKEFNGSVGLPFPSTDVAIF
                     DETDRELPVGEVGEICVRGPQVMAGYWGKPDETAKVMFGDGWLRTGDIGRLDANGYLF
                     VEDRKKDVIVVSGFKVYPNEIEDVATMQAGVREAAAIGVPDASSGEAVKLFVVRSDDK
                     LTTADVLAHARRHLTGYKVPRHIEFVASLPKSNVGKVLRRALKESEQAQENASAPK"
     gene            complement(1722..2357)
                     /locus_tag="VFL84_11885"
     CDS             complement(1722..2357)
                     /locus_tag="VFL84_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HET7609370.1"
                     /translation="MLIFRYLAALLALTVVCLPAAHGAEQTIVVVGDSLSSGYGIAAE
                     QSWVAMLEDRLQTEGYGYSVVNASIAGDTSAGGLARLPRLLAAHDPSLVVIELGGNDG
                     LRGQPVATLRDNLAKMIELSKQSGAGVVLAGIQIPPNYGTKYTEALAAVYPELARRFD
                     APLIDFILEDVALHRELMQPDGIHPNAAGQKIVFANVWRVLGPLLTPHDAG"
     gene            2350..3033
                     /locus_tag="VFL84_11890"
     CDS             2350..3033
                     /locus_tag="VFL84_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7609371.1"
                     /translation="MSTAEFLLRAENLTKKVSSPEGELTILDSVTIGVPRGHTAAVVG
                     PSGAGKSTLLALLAGLDEPTSGKVWLNGAELTALDEDGRAGVRNREVGFVFQSFHLLP
                     SLTAIENVLLPLELAATANASARARQALVDVGLEHRLRHYPKQLSGGEKQRVAIARAF
                     VTGPSLLFADEPTGNLDNKTGAKILDMLFDLNTAAGTTLVLVTHDLEAAGRCRHVIEL
                     AGGKVVKDG"
     gene            3030..3512
                     /locus_tag="VFL84_11895"
     CDS             3030..3512
                     /locus_tag="VFL84_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HET7609372.1"
                     /translation="MSAERFWRHKKLVELTPREWESLCDGCGRCCLKKLQDEATGKVV
                     YTDVACKLLDRERCRCRRYASRHALVPDCIALDAADEHAFDWLPTTCAYRKVASGQDL
                     DWWHPLVSGDSSSVHRAGISVRGRTLAERDVSVDELDARVIRWIKTAPLRRPTARRRT
                     "
     gene            complement(3509..>5464)
                     /locus_tag="VFL84_11900"
     CDS             complement(3509..>5464)
                     /locus_tag="VFL84_11900"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET7609373.1"
                     /translation="PILVQFGTWVGGDMDGNPHVTAKSIRHTLARQRALVLDLYYREC
                     AELAAHLSQGLRRVGVSEELLRRTEQYTGHFPEAAASLPSRHRQMPYRAYLRLVAARL
                     KATYDDHAYPYESPNELVADLELLADSLRANKGRNAGLFAVRRLLRRVRTFGFYLATV
                     DIRQNALVHRRVIGEGLQEPSWLSADDEQRARRLEEALERRESPLGALSSEGRRTLAV
                     FQTIAHCRRKYGRESIGPYIVSMAHGPDDVLSVLLLAKWGHLGSKGESVPLDIAPMFE
                     TGEDVERAPKIMERLLSDKLYREHLRARGDHQIVKLGYSDSHRDGGIAATRWSLQKAQ
                     QALVATMARHGVALTIFHGRGGTVSRSGGAQHEAVLAAPRGAISGRLRMTEQGENINT
                     KYGLRGIALRTLEQLLSAVLLVTARPPPENPAEARWQSIMEEIANASRGAYQSLLSDP
                     DDFIEYFRAATPIDVIERIGSRSERAELSDSATELRAAPWVFAWTQSRCLLPSWYGVA
                     TGLKKAIDAHGEEVLVEMFEQWHFFRVLLSDVATALAKADLDVAARYSVLAGERHEKF
                     FPAIRREYRACVDLILRLTRQNELLEASRTLRRAIRLRNPYVDPMSFLQVDLLERWRA
                     GGRQDGPVLQALTASINGIAHAMQGTG"
BASE COUNT          878 a         1878 c         1847 g          861 t
ORIGIN      
        1 acctcgccga cgggtagctc gcggtcggtc tcgtgctcta cttcggcgcg ctcgcgtttt
       61 cctgagcttg ctcgctctcc ttgagcgcgc gccgcagcac cttgccgacg ttcgatttcg
      121 gcaggctcgc gacgaactcg atgtgccgcg gcaccttgta gccggtcaga tggcgccgag
      181 cgtgcgcgag cacgtcggcc gtcgtcagct tgtcgtcgct acgcacgacg aacagcttga
      241 ccgcctcgcc cgacgatgca tccggcacgc caatggcggc cgcctcgcgg acgccggcct
      301 gcatcgtcgc gacgtcctcg atctcgttcg gatagacctt gaagcccgag acgacgatga
      361 cgtccttctt gcggtcctcg acgaagagat acccgttcgc gtcgagccgg ccgatgtcgc
      421 ccgtgcgcag ccacccgtcg ccgaacatga ccttggccgt ctcgtcgggc ttgccccaat
      481 acccggccat gacctgcggg ccgcgcacgc aaatctcgcc gacctcgccg acgggtagct
      541 cgcggtcggt ctcgtcgaag atcgcgacgt ccgtcgatgg gaatggcagg ccgaccgagc
      601 cgttgaattc cttcaggtgc agcgggttgc ccgtgacgat cggcgacgcc tccgtgaggc
      661 cgtacccctg cgagatgacg acgcccgtca ccgcctgcca gcgcgcggcg acctcgcgct
      721 gcacggccat gccgccgccc atgacgacgc gcagcgacga aaaatcgagc ttggcgaacc
      781 cgtccgtgtg gagcagcgcg ttgaacagcg tgttgacgcc cgtcatgaac gcgaacttgt
      841 gccttcgcag cagcgcgacg aagcccttga aatcgcgcgg atcggtgatc agcaggttct
      901 gcgcgccgag ctcgacgaag cagaagagat tcgccgtcag cgaatagacg tgatagagcg
      961 gcagcggcgt cacgacgacg ccggcgtcgg gcgtgtagaa cggccgcgcc caggcggctg
     1021 cttgcagcgt gttcgcaacc atgttgcgct gcgtcagcat cgcgcccttg gcgcgccccg
     1081 tcgttccgcc cgtgtattgc aggaacgcga ggtcgccgcc gccgagccgc ggcgccgtgt
     1141 aggcgagcgc ggcgccgcgc gccagcgcgt cgcgatacgc gacggcgccc gggatttgcc
     1201 acggacgcac gagcttcttg acgtgcttga cgacaaagtt gacgacggcg cgcttgagcg
     1261 gcgagcagtg atcgccgagc cgcgtcacga tgacggtttg gacgttcgtc tgcggcaacg
     1321 cggcgccgac cgtgtgagcg aaattctcga gcacgacgac ggcgcgggcg ccactgtccg
     1381 cgagctggtg cccgagctcc gtgatcgtgt acatcggatt gacgttcacg gccaccatgc
     1441 cggcgcgcag gatgccgaac agcgctacgg gcgactgcag cgtgttcggc agcatgatcg
     1501 cgacgcggtc cccgcgctcg agcccaagcg cctgcaagta agcggcgaac gcgcgcgaca
     1561 gccgctcgac ctcgcggaac gagagcgtcg tgccgagatt ggtgaaagcc ggctgggccg
     1621 agaaccgcgc gaagctcgct tcgagcagcg cgacgagcga cggatagcgg tccgggtcga
     1681 tctcggccgg gacacccggc gagtacgact tgagccacgg tttagccggc atcgtgcggc
     1741 gtcagcagcg gtccgagcac gcgccagacg ttcgcgaaca cgatcttctg gcccgccgcg
     1801 ttcggatgaa tgccgtccgg ctgcatgagc tcgcggtgga gcgcaacatc ctccaggatg
     1861 aagtcgatga gcggcgcgtc gaagcgtcgg gcgagctcgg gatacacggc ggccagcgcc
     1921 tccgtgtact tcgtcccgta gttcggcggg atctgaatgc ccgcgagcac gacgccggcg
     1981 ccgctttgct tgctgagctc gatcatcttc gcgagattgt cgcgcagcgt cgcgacgggc
     2041 tggccgcgca gcccgtcgtt gccgccgagc tcgatgacga cgagcgacgg atcgtgggcg
     2101 gccagcaaac gcggcaaacg cgcgagcccg cccgccgacg tgtcgcccgc gatgctggca
     2161 ttcaccacgc tatatccgta accttccgtc tgcaggcgat cctcgagcat cgcgacccag
     2221 gactgctccg cggctatccc atagccgctg ctcagggagt cgcccaccac gacgatggtt
     2281 tgctcggcgc cgtgcgctgc cggaaggcag acgaccgtca gcgcgagcaa agcagcaagg
     2341 tagcggaaga tgagcaccgc ggaattcctg ttacgagccg aaaatctgac gaaaaaggtt
     2401 agcagccccg agggtgagct gaccatcctc gattccgtca cgatcggcgt gccgcgcggg
     2461 cacacggcgg ccgtcgtggg gccgtcgggt gcgggcaagt cgactctgct cgcgctgctc
     2521 gcgggcctcg acgagccgac gtccggaaaa gtctggttga atggcgcgga gctgacggcg
     2581 ctcgacgagg acggccgggc cggcgtgcgc aatcgagagg tcggtttcgt ttttcaatct
     2641 tttcacctgt tgccgtcatt gacggcgatc gagaacgtgc tcctgccgct cgagctcgcg
     2701 gcgaccgcga acgcctcggc gcgggcccgg caagcgctcg tggacgtggg cctcgagcat
     2761 cgcctgcgcc actatccaaa gcagctgtcg ggcggcgaaa agcagcgcgt cgcgatcgcg
     2821 cgcgcgttcg tgacggggcc gtcgctgctc ttcgccgacg agccgaccgg gaatctcgac
     2881 aacaagaccg gcgccaagat cctcgacatg ctgttcgacc tgaacacggc ggccggcacg
     2941 acgctcgtgc tcgtcacgca cgacctggag gcggcgggcc gctgccggca cgtcatcgag
     3001 ctcgcgggcg gcaaagtcgt gaaagacgga tgagcgcaga gcggttctgg cggcacaaga
     3061 agctcgtcga gctgacgccg cgcgagtggg aatcgctctg cgacggctgc ggccggtgct
     3121 gcctgaaaaa gcttcaagac gaagcgacgg gcaaggtcgt ctacacggac gtcgcgtgca
     3181 agctgctcga ccgcgagcgt tgccgctgcc gccgctatgc gagccgccac gctctcgttc
     3241 cggactgcat cgcgctcgat gcggccgacg agcacgcgtt cgattggctg ccgacgacat
     3301 gcgcgtatcg caaagtcgcg tccggccagg acctcgactg gtggcatccg ctcgtctcgg
     3361 gcgattcaag ttccgtgcac cgcgcgggaa tttccgtgcg cggccgcacg ctggccgagc
     3421 gcgacgtaag cgtcgacgag ctcgacgcgc gcgtcatccg ctggatcaag acggcaccgc
     3481 tccgccggcc gacggcccgc cggcgaacct agcccgtccc ttgcatcgcg tgcgcgatgc
     3541 cgttgatgct cgccgtcagc gcttgcagga ccggcccgtc ctgccgccca cccgcgcgcc
     3601 agcgctcgag caggtcgacc tgcaggaagc tcatcggatc gacgtacggg ttgcgcaagc
     3661 gaatcgcgcg ccgcagcgtg cgcgacgctt ccaagagctc gttctgccgc gtgagcctca
     3721 ggatcaagtc gacacaggcg cgatactcgc gccggatcgc cgggaagaat ttctcgtgcc
     3781 gctcgcccgc tagcaccgaa tagcgcgccg cgacgtctag atcggccttc gcgagcgccg
     3841 tcgcgacgtc ggacagcagc acgcggaaga aatgccattg ctcgaacatc tcgacgagca
     3901 cttcttcgcc gtgcgcatcg attgccttct tcaagcccgt cgcgacaccg taccagctcg
     3961 gaagcaaaca gcggctctga gtccacgcga atacccacgg agccgcacgc agctccgtcg
     4021 cggagtcact gagctcggcg cgttcggagc ggctgccgat ccgctcgatg acgtcgatcg
     4081 gcgtcgcggc gcggaagtac tcgatgaaat cgtcgggatc cgacagcaag ctctgatagg
     4141 cgccgcggct cgcgttggcg atctcctcca tgatggattg ccagcgcgcc tcggcgggat
     4201 tttccggcgg cggccgcgcc gtgacgagca gcaccgcgga cagcagctgc tcgagcgttc
     4261 gcagcgcgat gccgcgtagg ccgtacttcg tgttgatgtt ctcgccctgc tccgtcatgc
     4321 gcagccggcc cgagatcgcg ccgcgcggcg cggcgagcac ggcctcgtgc tgggcgccgc
     4381 cgctgcggct cacggtgccg ccgcggccgt gaaagatcgt cagcgcgacg ccgtgccgcg
     4441 ccatcgtcgc gacgagcgct tgctgcgcct tttgcaggct ccagcgcgtc gcggcgatgc
     4501 cgccgtcgcg gtgactgtcc gagtacccga gcttgacgat ctggtgatcg ccgcgtgcgc
     4561 gcagatgctc gcgatagagc ttgtcgctca acaggcgctc catgatcttc ggcgcgcgct
     4621 cgacgtcctc gcccgtctcg aacatgggcg cgatgtcgag cggcacggac tcgcctttcg
     4681 agcccaagtg gccccacttc gcgagcagca gcaccgacag gacgtcgtcg ggcccatgcg
     4741 ccatgctgac gatgtacggg ccgatcgact cgcgcccgta tttgcgccgg cagtgcgcga
     4801 tcgtctgaaa cacggcgagc gtgcggcggc cctccgacga cagcgcgccg agcggcgatt
     4861 cgcgccgctc gagcgcttcc tcgagccgcc gcgcgcgctg ctcgtcgtcg gccgaaagcc
     4921 agctcggctc ctgcagcccc tcgccgatca ctctcctgtg cacgagcgcg ttctggcgga
     4981 tgtcgacggt cgccaagtag aacccgaacg tccgcacgcg gcgcagcagc cggcggaccg
     5041 cgaacaggcc cgcattgcgg cccttgttcg cgcgcaggct gtcggcgagc agctcgagat
     5101 ccgcgacgag ctcgttcggc gactcgtacg gataagcgtg gtcgtcgtag gtcgccttga
     5161 gccgcgcggc gacgaggcgc aagtacgctc gatacggcat ttgccggtgg cgcgacggca
     5221 gcgacgcagc ggcctcgggg aagtgccccg tgtactgctc cgtgcgccgc agcagctcct
     5281 ccgacacgcc gacgcgccgg aggccctggc tcaagtgcgc ggcgagctcg gcgcattcgc
     5341 ggtagtaaag atcgagcacg agcgcgcgct gccgcgcgag cgtgtggcga atcgacttcg
     5401 ccgtcacgtg cggattgccg tccatgtcgc cgccgaccca ggtgccgaac tgcacgagga
     5461 tcgg
//
LOCUS       DASSNN010000639         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_612379, whole genome shotgun sequence.
ACCESSION   DASSNN010000639 DASSNN010000000
VERSION     DASSNN010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612379"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VFL84_11905"
     CDS             complement(<1..1043)
                     /locus_tag="VFL84_11905"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET7609374.1"
                     /translation="MTDSADDARSTIERRTFLQLAGGVALAGATQWASTAAAAPSKPY
                     VPGASTHEYDKMFVASVTPYKPGSEDIDEAAFRDYMRYWAQPKFVGAGGAMLVSPEAG
                     EAFYLTQPEKLRLAEIALDELGDKTVVFSGVMQTTTRACLEEARALKKLGVHGLFLMP
                     PIGTIDIVAAWDSQKNPEVWIDQLKEIADAVNLPVIIHPTGGKFFALPLESGMKAVDA
                     VPQIVGYKMITQDFEAMARALRAYTKRHVAVLPAGANNMPTAIKNGWFDGTVSGSWNY
                     AMEKTVDAIVAERDQKDPAKAEKLWGDGLMALHRTVTNSDKGYRLHSAYKLCTWMRGL
                     IPNPFLRAPQRRLP"
     gene            1161..>2135
                     /locus_tag="VFL84_11910"
     CDS             1161..>2135
                     /locus_tag="VFL84_11910"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HET7609375.1"
                     /translation="MNGPTRSPFFISVLAALVLVAPAAAQTDGAERPTIEEITVIGRY
                     PGPPLWKVTSGARTLWVFGELSPVPKGLDWDPRNAERVLDRAGVVIGSPRVSAMTLNP
                     IRVFKLFRGARRLVRLPEDATLADQMPPELYARYAALRTRYLRKVDEEDEELRPALAV
                     LQLYRAALDDTGLTSDSGIGKTLARKMRRSKADEAEVIVETEPETVLDELEKITPEAE
                     LSCFESVLTTIETDLGGMKERANAWAVGDVEALKRFDYPDSQGNCLDALVTVEGFAEL
                     RDELYRKWLSEAERALATHATSFSVLPMRELVASDGLLAQLAARGYTVT"
BASE COUNT          336 a          718 c          709 g          372 t
ORIGIN      
        1 ggcaagcgcc gctgcggcgc gcgcaggaac ggattcggaa tcaggccgcg catccaggtg
       61 cagagcttgt acgcgctgtg aaggcgatag cccttgtcgg aattcgtgac cgtgcgatgc
      121 agcgccatca agccgtcgcc ccacaacttc tccgccttgg ccgggtcctt ctggtcgcgc
      181 tcggcgacga tcgcgtcgac ggtcttctcc atcgcgtaat tccacgagcc gctcaccgtg
      241 ccgtcgaacc agccgttctt gatcgcggtc ggcatgttgt tggcgccggc gggcaggacc
      301 gcgacgtggc gcttcgtgta ggcgcgcagg gcgcgcgcca tcgcttcgaa gtcctgcgtg
      361 atcatcttgt agccgacgat ctgcggcacc gcgtcgacgg ccttcatgcc ggactcgagc
      421 ggcaacgcga agaatttgcc gcccgtcgga tggatgatga cgggcaggtt cacggcatcg
      481 gcgatttctt tgagttgatc gatccagact tcggggttct tctgcgaatc ccacgccgcg
      541 acgatgtcga tcgtgccgat cggcggcatc aggaacaggc cgtgcacgcc gagcttcttc
      601 agcgcgcgtg cctcttcgag gcaggcgcgc gtcgtcgtct gcatgacgcc gctgaagacg
      661 acggtcttgt cgccgagctc gtcgagcgcg atctcggcta agcgaagctt ctcgggttgc
      721 gtgaggtaga acgcctcgcc ggcttccggc gaaaccagca tggcgccgcc ggcgccgacg
      781 aatttcggct gcgcccaata gcgcatgtaa tcgcggaacg cggcttcgtc gatgtcttcg
      841 ctgcctggct tgtacggcgt gacgctcgcg acgaacatct tgtcgtactc gtgcgtgctc
      901 gcgccgggca cgtacggctt cgaaggcgct gccgcggctg tcgatgccca ctgcgtcgcg
      961 cccgccagcg ctacgccgcc ggcgagctgc aggaaggtcc gtcgctcgat cgtgctgcga
     1021 gcgtcatctg ccgagtcagt catggctccc ccttcggcac ccgcgagcgc cgccaggcct
     1081 ggcgaccgtc catacgggcc gttggcccga tgctacgccg caagtcgtac cagcagaagc
     1141 cccgttgcta agatcgcgcg atgaacggac cgacccgctc gccattcttc atctccgtgc
     1201 tcgccgctct cgtcctcgtt gcgccggcag cggcacaaac cgacggcgcc gaacgcccga
     1261 cgatcgaaga gatcaccgtg atcggccgct atccgggccc gccgctctgg aaggtcacgt
     1321 cgggcgctcg cacgctctgg gtgttcggcg agctgtcgcc cgtgccgaag ggactcgact
     1381 gggatccgcg caatgccgag cgcgtgctcg atcgcgccgg cgtcgtcatc ggcagcccgc
     1441 gcgtcagcgc gatgacgctg aacccgatca gggtgttcaa gctgttcaga ggcgcgcggc
     1501 ggctcgtccg gctgcccgaa gatgcgacgc tcgcggacca gatgccgccc gagctttacg
     1561 cacgttatgc ggcgctacgc acgcgctact tgcggaaggt cgacgaagaa gacgaagagc
     1621 ttcgtcctgc gctcgcggtg ctgcagctct atagggccgc gctcgacgat accggcctca
     1681 cgagcgactc gggtatcggc aaaacgctcg cgcgcaagat gcgccgctcg aaggccgacg
     1741 aagccgaggt catcgtcgag accgagcccg agaccgtgct cgacgagctc gagaagatca
     1801 cgccggaggc cgagctcagc tgcttcgagt ccgtgttgac gacgatcgag accgacctcg
     1861 gcggcatgaa ggagcgcgcg aacgcgtggg ccgtcggcga cgtcgaggcg ctgaagcgct
     1921 tcgactaccc cgattcgcaa ggcaactgtc tcgatgcgct ggtcaccgtc gagggcttcg
     1981 ccgagctgcg cgacgagctc taccggaagt ggctctccga ggccgagcgc gcgctcgcga
     2041 cgcatgcgac gtcgttctcg gtgctgccga tgcgcgagct cgtcgcatcc gacggattgc
     2101 tcgcgcagct cgcggcgcgc ggttataccg tgacg
//
LOCUS       DASSNN010000640         6077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_612461, whole genome shotgun sequence.
ACCESSION   DASSNN010000640 DASSNN010000000
VERSION     DASSNN010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612461"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFL84_11915"
     CDS             complement(<1..491)
                     /locus_tag="VFL84_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7609376.1"
                     /translation="MISVVTVHGLWMRGAAMGVLRRRLEPRGLMVHEFTYSSIAGSLA
                     DNTAALAAFVERVPGETVHFVGHSLGGLLICALLEHALPPRIGRVVCLGTPFKGSKTA
                     ARVTRWPGGRHVIGKCLADVHARGGFAAWRAGVEAGCVAGRMPFGVGRLLGPFPEPND
                     GTVA"
     gene            complement(488..937)
                     /gene="nudB"
                     /locus_tag="VFL84_11920"
     CDS             complement(488..937)
                     /gene="nudB"
                     /locus_tag="VFL84_11920"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HET7609377.1"
                     /translation="MAAVAEFRRPESVLVVVHTPELDCLLLERVEPRGFWQSVTGTLR
                     WAETPAECAARELEEETGLSPHGLRDAHVQRRFPILPAWRSRYAPGVDSNVEHQWYLE
                     VPEVRAVEIEPTEHVAYLWLPVEAAIEKVASWTNREALQRLADARRA"
     gene            complement(945..2735)
                     /gene="aspS"
                     /locus_tag="VFL84_11925"
     CDS             complement(945..2735)
                     /gene="aspS"
                     /locus_tag="VFL84_11925"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET7609378.1"
                     /translation="MRSHYCGHVNRAEIGRTVTVAGWVHRRRDHGGVIFIDLRDREGL
                     LQVVFDPDRAEMFKEAERLRSEYVLAVEGTVRARPQGTVNPNLASGEVEVLAKKLTVL
                     ARAKTPPFHHDEPVSEELRLKYRYLDLRREPMLKNLRLRHAVTRALRKFMDEHGFVDV
                     ETPILTKTTPEGARDYLVPSRTQQGNFFALPQSPQLMKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQLDIETSFMNEEDILQLMEELLRDTFKSVLGAELPKPFPRMTYADA
                     MRRYGSDKPDLRNPLELVEVADLFRGVEFKVFAGPASDPKSRIAALRLPAGGELTRKE
                     IDDYTALVGRHGAKGLAYIKVNERAKGRDGLQSPILKFLPDAAIAGVLERTGAVDGDL
                     VFFGADKAGVVNESLNALRAALGRDRKLLKGEWAPLWIVDFPMFEELPEGGLTPVHHP
                     FTAPRLPGADAVSMTAEQLKAAPTAATARAYDIVLNGYEVGGGSIRIHNPELQSAVFD
                     LIGISKEQAELKFGFLLAALDFGCPPHGGIAFGLDRLVMLMAGADNIRDVIAFPKTAT
                     ASDLMTGAPSPADAAQLKELGIRLKSAAPG"
     gene            complement(2851..3258)
                     /locus_tag="VFL84_11930"
     CDS             complement(2851..3258)
                     /locus_tag="VFL84_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HET7609379.1"
                     /translation="MPIYEYRCSACGHDLEALQKMTDAPLRKCPECGKSQLKRLVSAS
                     QFRLKGSGWYETDFKNKSETKRNLVEHAGADSSDAADKSTDTAAKDEAGKAVAAAKNG
                     GATEKPAKKPASEKPAPVAPARKAKARPKAARG"
     gene            complement(3278..5869)
                     /gene="clpB"
                     /locus_tag="VFL84_11935"
     CDS             complement(3278..5869)
                     /gene="clpB"
                     /locus_tag="VFL84_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018946531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HET7609380.1"
                     /translation="MDKLTTNFQQALADAQSLAVGRDHQFIEPVHVMAALLEQPGGSV
                     VGILTKAGANLNLLRSQLGEAIDRIPSVEGTPGEVHIGNELSKLLNVMDKLAQERGDA
                     YISSELFLLAAVQSKTDLGRILERAGAVKGAIAKAIDEVRGGAKVEDQNAEEKRQALE
                     KYTIDLTARAEQQKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVNGQVPEGLKGKRVLSLDMGALIAGAKFRGEFEERLKAVLNDLAKQEGQVILFID
                     ELHTMVGAGKAEGSMDAGNMLKPALARGELHCVGATTLDEYRKYVEKDAALERRFQKV
                     LVDEPSVEDTIAILRGLKERYEVHHQVEITDPAIVAAATLSHRYISDRQLPDKAIDLI
                     DEAASRIRMEIDSKPEEMDRLERRLIQLKIEREALNKESDEASKRRLADLEAQIAKLD
                     REYADLEEVWKAEKALVQGEAQIKEELERARAELEKARRASDLGRMAELQYGKIPELE
                     RRLEAAIKSDRGAANKLLRNKVTDEEVAEVVSKWTGIPVSKMLEGEKEKLLRMEQHLE
                     ARVVGQTEAVQTVSNAIRRARAGLSDPNRPNGSFLFLGPTGVGKTELTKALAAFLFDT
                     EEAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVVLLDEVEKAH
                     PDVFNVLLQVLDDGRLTDGQGRTVDFRNTVIVMTSNLGSQRIQELAGAANYARMKAEV
                     MEIVGQHFRPEFINRVDDVVVFHPLEQEHIRKIVDIQLGYLQRRLADREIGLVLDASA
                     RDRLAQAGFDPVYGARPLKRAIQQQLENPLAQRILRGDFGPGDVVRVVAEGDALGFVK
                     GVNEEAA"
BASE COUNT          928 a         2105 c         1950 g         1094 t
ORIGIN      
        1 gcgaccgtgc cgtcgttcgg ctccgggaac ggaccgagca ggcggccgac accgaacggc
       61 atgcggcctg cgacgcagcc ggcctcgacg ccggcgcgcc acgccgcaaa cccgccgcgc
      121 gcgtgaacgt ccgccaagca cttgcctatg acgtggcggc caccgggcca acgcgtgacg
      181 cgcgccgcgg tcttgctgcc cttgaacggc gtgccgaggc acacgacgcg gccgatacgc
      241 ggaggtaacg cgtgctcgag caacgcgcag atcaagaggc cgcccaagct gtggccgacg
      301 aagtgaacgg tctcgccggg gacgcgctcg acgaacgcag cgagcgccgc cgtgttatcc
      361 gccagcgagc ccgcgatcga cgagtacgtg aactcgtgaa ccatgaggcc gcgaggctcg
      421 agccggcggc gtagaacgcc catcgcggcc ccgcgcatcc agagcccgtg caccgtgacg
      481 accgagatca cgcgcggcgc gcgtcggcga ggcgctgcag cgcctcgcga ttcgtccacg
      541 acgcgacctt ctcgatggcg gcctcgaccg gcagccacag atacgcgacg tgctcggtcg
      601 gctcgatctc gaccgcgcgc acttcgggca cctcgagata ccactgatgc tcgacgttcg
      661 agtcgacgcc gggcgcatag cgcgaccgcc acgcgggcag aatcggaaag cggcgctgca
      721 cgtgcgcgtc gcgcaagccg tgggggctca agcccgtctc ttcctcgagc tcgcgcgcgg
      781 cgcactcggc cggcgtctcg gcccagcgca acgtgcccgt gaccgactgc cagaagccgc
      841 gcggctcgac gcgctcgagc agcaagcagt cgagctccgg cgtgtgcacg acgacgagca
      901 cggactccgg tcgcctgaac tctgcgacgg cggccatgcg ctcgtcagcc cggagcggcg
      961 ctcttcaagc ggatgccgag ctccttgagc tgcgcggcat cggccgggct cggcgcccct
     1021 gtcatcaaat cgctcgccgt ggcggtcttc gggaatgcga tgacgtcgcg gatgttatcg
     1081 gcgcccgcca tcagcatcac cagtcggtcg aggccgaacg caatgcctcc atgcggcggg
     1141 cagccgaagt cgagtgccgc cagcaggaac ccgaacttga gctcggcctg ctccttcgag
     1201 ataccgatca agtcgaacac ggccgactgc agctcggggt tgtggattcg gatcgagccg
     1261 ccgccgacct cgtagccgtt cagcacgatg tcgtaggcgc gcgccgtcgc cgcggttggc
     1321 gccgctttta gctgctcggc cgtcatcgac acggcgtcgg cgccgggcag gcgcggcgcc
     1381 gtgaacggat gatgcacggg cgtgaggccg ccctccggca gctcctcgaa catcggaaag
     1441 tcgacgatcc agagcggcgc ccattcgcct ttcagcagct tgcggtcgcg gccgagcgcc
     1501 gcgcgaagcg cgttcagcga ttcgttgacg acgcccgctt tgtccgcgcc gaagaacacg
     1561 aggtcgccgt cgaccgcgcc ggtccgctcg agcacgccgg cgatcgccgc atcgggcagg
     1621 aacttcagta tcggcgactg caagccgtcg cggcctttcg cgcgctcgtt gaccttgatg
     1681 tacgcgagac ctttggcgcc gtgccggccg acgagagccg tgtagtcgtc gatctccttg
     1741 cgcgtgagct cgccgcctgc aggcaagcgc agcgccgcga tgcggctctt cgggtcggac
     1801 gccggacccg cgaacacttt gaactcgacg ccgcggaaga gatccgcgac ctcgacgagc
     1861 tcgagcgggt tgcgcagatc gggcttgtcg ctgccgtaac ggcgcattgc gtccgcgtac
     1921 gtcatgcgcg ggaacggctt cggtagctcc gcgccgagca cggacttgaa agtgtcgcgc
     1981 agcagctcct ccatcaattg aaggatgtcc tcctcgttca tgaagctcgt ctcgatatcg
     2041 agctgcgtga actcgggttg gcggtccgcg cgcaggtcct cgtcgcggaa gcacttgacg
     2101 atctgatagt agcggtcgaa gcccgacatc atcagcagct gcttcatgag ctgcggcgat
     2161 tgcggcagcg cgaagaagtt gccctgctgc gtgcggctcg gcacgagata gtcgcgcgcg
     2221 ccctcgggcg tcgtcttcgt caggatcggc gtctcgacgt cgacgaatcc atgctcgtcc
     2281 atgaacttcc gcagcgcgcg cgtcacggcg tgccgcaacc gcaagttctt cagcatcggc
     2341 tcgcggcgca gatcgagata gcggtacttg agccgcagct cctcgctgac gggctcgtcg
     2401 tgatggaacg gcggcgtctt cgcgcgcgcg agaaccgtga gcttcttcgc gagcacctcg
     2461 acctcgccgc tcgcgagatt cgggttcacg gtgccttgcg gccgcgcgcg caccgtgcct
     2521 tcgacggcga gcacgtactc cgagcgcagc cgctcggcct ctttgaacat ctcggcgcgg
     2581 tccggatcga agacgacctg cagcaggccc tcgcgatcgc gcaagtcgat gaagatcacg
     2641 ccgccgtgat cacggcgccg gtgtacccac ccggcgaccg tgacggtgcg gccgatctcg
     2701 gcacggttga cgtggccgca gtagtgactg cgcatcgctc tggtcctttt gagcctaacc
     2761 ctttgaaacc aaaggtggca tgagcctcga agaggccgcg atgttacgcg ggaaggcgcg
     2821 gcgcgcgcaa ggcgcgcgga ccgaagccgg ttagccgcgc gccgcttttg gccgagcctt
     2881 cgctttccgc gcgggcgcga ctggagcggg cttctcgctc gccggcttct tcgccggctt
     2941 ctctgtggcg ccgccgttct ttgcggccgc gacggcttta cccgcttcgt ccttcgcggc
     3001 ggtatcggtc gacttgtccg ctgcgtcgct cgagtccgcg ccggcgtgct cgacgagatt
     3061 gcgcttcgtc tcgctcttgt tcttgaaatc ggtctcgtac cagccgctgc ctttcaagcg
     3121 gaactgcgac gccgagacga gcctcttcag ctgcgacttg ccgcactcgg ggcacttgcg
     3181 cagcggcgca tccgtcatct tctgcaacgc ttccagatcg tgcccgcagg cactgcatcg
     3241 atactcgtag atcggcatac ggcttaggct ctcaacctca cgcggcttct tcgttgacgc
     3301 ccttcacgaa tccgagcgcg tcgccctctg cgacgacgcg gacgacgtcg ccggggccga
     3361 aatcgccgcg caggatacgc tgtgcgagcg gattctccaa ttgctgctga atcgcgcgct
     3421 tcagcggccg cgccccgtag acggggtcga accccgcttg cgcgaggcga tcgcgcgccg
     3481 aagcgtccag cacgaggcca atctcgcggt ccgcgagccg ccgttgcaga tagccgagct
     3541 gaatgtcgac gatcttgcgg atgtgctcct gctcgagcgg gtggaatacg acgacgtcgt
     3601 cgacacggtt gatgaactcg ggccggaagt gctgaccgac gatttccatg acctcggcct
     3661 tcatgcgcgc gtagttcgcc gcgcccgcga gctcctggat gcgttggctg cccaagttcg
     3721 acgtcatgac gatgacggta ttgcggaagt ccacggtgcg gccctggccg tccgtgaggc
     3781 ggccgtcgtc gagcacttgc agcagcacgt tgaagacgtc ggggtgggct ttctcgacct
     3841 cgtcgagcag cacgaccgag tacggcttgc gccgcacggc ctcggtcagg tacccgcctt
     3901 cctcgtagcc gacgtagccc ggcggcgcgc cgatcaagcg cgcgaccgag tgcttctcca
     3961 tgaattccga catgtcgatg cggaccatgg cctcctcggt gtcgaacagg aacgcggcga
     4021 gcgccttcgt gagctccgtc ttgccgacgc cggtcgggcc caagaacagg aacgagccgt
     4081 tcggccgatt cggatccgac aggcccgcgc gcgcgcggcg gatcgcgttc gacacggtct
     4141 gcacggcttc ggtctggccg accacgcgcg cctcgaggtg ctgctccatg cgcaacagct
     4201 tctctttctc gccctcgagc attttcgaga cggggatgcc ggtccacttc gagacgacct
     4261 cggcaacctc ctcgtccgtg actttgttgc gcagcaattt gttggccgcg ccacggtcgc
     4321 tcttgatcgc ggcttcgagc ctacgctcga gctcggggat tttgccgtac tgaagctcgg
     4381 ccatgcggcc caagtccgac gctcgccgcg ccttctcgag ctcggcgcgc gcgcgctcga
     4441 gctcctcctt gatttgcgcc tcgccttgca ccagggcctt ctcggccttc cagacttcct
     4501 cgaggtccgc gtactccctg tcgagcttcg cgatctgcgc ctcgagatcc gcgaggcggc
     4561 gcttcgacgc ctcgtccgat tccttgttca atgcctcgcg ctcgatcttg agctggatga
     4621 gccggcgctc gagtctgtcc atctcctcgg gcttcgaatc gatctccatg cgaatgcgcg
     4681 acgccgcctc gtcgatcaag tcgatcgcct tgtccggcag ctgccggtcg ctgatgtagc
     4741 ggtgcgacag cgtcgcagcc gcgacgatcg cgggatccgt gatctcgacc tggtgatgaa
     4801 cctcgtagcg ttccttcaag ccccgcagga tcgcgatcgt gtcctcgacg ctgggctcgt
     4861 cgacgagcac cttctggaac cgccgctcga gcgcggcatc cttctcgacg tacttgcggt
     4921 actcgtcgag cgtagtcgcg ccgacgcaat gcagctcgcc gcgcgcgagc gcgggcttca
     4981 gcatgttgcc ggcatccatc gagccttcgg ccttgccggc gccgaccatc gtgtgcagct
     5041 cgtcgatgaa cagaatcacc tgcccttcct gcttcgcgag atcgttgagc acggccttca
     5101 gccgctcctc gaactcgccg cggaacttcg cgccggcgat cagcgcgccc atgtcgagcg
     5161 acaacacgcg ctttcccttc aagccttcgg gaacctgacc gttgacgatg cgttgcgcga
     5221 gaccctcgac gatcgccgtc ttgccgacgc cgggctcgcc gatcagcacc ggattgttct
     5281 tcgtgcgccg ctgcagcact tggatcgtgc ggcggatctc gtcatcgcgg ccgatgacgg
     5341 gatcgagctt ctgctgctcc gcgcgagcgg tcaggtcgat cgtgtacttc tcgagcgcct
     5401 gacgtttttc ctcggcgttc tggtcctcga ccttcgcgcc gccgcgcacc tcgtcgatcg
     5461 ccttcgcgat cgcgcctttg accgcgccgg cacgctcgag aatccgcccg agatcggtct
     5521 tcgactgcac ggccgcgagc aagaacagct cgctggaaat gtaggcatcg ccgcgctcct
     5581 gcgcgagctt gtccatgacg ttcaagagct tcgagagctc gttgccgatg tgcacctcgc
     5641 cgggcgtccc ttcgacgctt gggatgcgat cgatcgcctc gccgagctgc gagcgcagca
     5701 gattcaggtt ggcgccggcc ttcgtgagga tgccgaccac gctgccgccc ggctgctcga
     5761 gcagcgccgc catcacgtgg acgggctcga tgaattgatg gtcgcggcca acggcaagcg
     5821 actgcgcgtc ggcaagcgcc tgttggaagt tcgtcgtcag cttgtccatg cgcatagcaa
     5881 agcgctccgc tcgttccgca ggctcgtcac atgagggccg ccccagctct tctcaaccac
     5941 ccccgccgaa tggggacaga tctattttcg caaaggggac ggaccccatt ttccgaaaat
     6001 agatctgtcc ccatttgtgg tccccatttg cgagcctagg cggaggagtc gagccatacc
     6061 agcgtggcca tgcggcc
//
LOCUS       DASSNN010000641         8288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1239716, whole genome shotgun sequence.
ACCESSION   DASSNN010000641 DASSNN010000000
VERSION     DASSNN010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239716"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VFL84_11940"
     CDS             complement(<1..515)
                     /locus_tag="VFL84_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609381.1"
                     /translation="MEPITAPPNPFVAETGSLVIPSFEELFFLPGDWFIYLLSSRAAP
                     IAETLGISPADYGGTFAGFIAWFSWILLAIVAIAATSGVRRFDRALTGHIIYAGSEVR
                     RRWRMALVFADYRRKLRVQRKEPSIDVAEEPGLSRDELRILQLHAKLSPGFALSTRDV
                     AEELNTRVHDIR"
     regulatory      complement(516..591)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(650..907)
                     /locus_tag="VFL84_11945"
     CDS             complement(650..907)
                     /locus_tag="VFL84_11945"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HET7609382.1"
                     /translation="MRDQDLEALLAELHLRLKQARSLDPEALKLLTTVARDIETALEN
                     DDASAVATEPVEALAVRFEAEHPSLAGVLRQIMDTLAKAGI"
     gene            999..2318
                     /locus_tag="VFL84_11950"
     CDS             999..2318
                     /locus_tag="VFL84_11950"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET7609383.1"
                     /translation="MLRAALLALAMLTASNARAQPLFEELRLIAPAAPGGGWDQTARV
                     MQQALQRERIVTTPVVENVPGAAGTIGLARFVGAERGNGDVLLVSGLIMLGGIVTYDS
                     PVTLADAVPIARLTGEYEALAVPAASPFRTLEDLLAAFRTDPESISWGGGSVGGSDQI
                     LAGLIAAAVGVEPRRVNYIGYSGGGEALAAVLGGQVSVGVNGLAEFAAQVDAGALRVL
                     AVSSGERLPGVDAPTLRERGVDVELENWRSVLAPPGITAGERARLAAAVDAMVRSNEW
                     RAMLARYRWLDRYLAGDEFAAFAAAEERRVRSILRDLGASAAAGDARAFDPYAAFVLT
                     GLALFGVAASVSVLRARNSAHERAPLPWRPLALIGAGAALHVLLAETLGFIVAAAVLF
                     WLVARAFDTRHPARDLGIALAVAAVSYALFDYALDLPLPAGVLGAWL"
     gene            2322..3815
                     /locus_tag="VFL84_11955"
     CDS             2322..3815
                     /locus_tag="VFL84_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014332771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HET7609384.1"
                     /translation="MDTFEGLSFGFANALTPQNLLWALLGTTLGTAIGVLPGIGPALT
                     VAVLLPVTFGLDPVSAFIMFGGIYYGAMYGGSTTSILLNTPGETGAIATAIDGHQMAL
                     RGRGAAALTTAALGSFVAGTIATLALALFAPALASVALEFGPAEYFALALLAFSAVTA
                     LLGASLARGLFSLFLGLALGLVGIDQLTGQARLSFGIPQLLDGIDVVILVIGLFAVGE
                     TLHQAARPSRAEAAPVQLTRFMSMSREDWRRSWKAWLRGTAIGFPLGILPMGGSVIPT
                     FLSYYAEKRLSPKPSEFGHGAIEGVAGPEAANNASTAGVLVPLLTLGVPSSATAAILL
                     AAFQQYGLQPGPLLFSTRPDLVWTLIASLVLGNVMLLVLNLPLVSVWARVMLLPRPLL
                     FGVVLVLASLGVYSLNRSLLDVGLLYAVGALGLALRVLDVPLVPAVLGLVLGPMAEQQ
                     FRRAVAISEGDLTVFVTRPISAALLTAAALALLAPALLKRRRRRRHE"
     gene            3808..5205
                     /gene="tcuA"
                     /locus_tag="VFL84_11960"
     CDS             3808..5205
                     /gene="tcuA"
                     /locus_tag="VFL84_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HET7609385.1"
                     /translation="MSDTGAPYDVVVIGGGNAGLSAALTARRAGARVLVLERAPRELR
                     GGNSRHTRNLRCAHAAPTDILTGSYPGEELLADLARINDGETDEALAALVAARSSDCV
                     PWMAAFGARFQAALRGTLQLSRTNAFFLGGGTALMNSYYAAAEKLGIDVRYDAEVIEL
                     VLRDGKFESALVRSRGAEQAVRARAVVVAAGGFEANLEWLRDAWGDAARNFLIRGTPY
                     NTGTMLRALLDAGAAPIGSPSECHAVAIDGRAPKFDGGIVTRLDCLPLGIVVDRDAQR
                     FYDEGEDVWPKRYAIWGKLVARRPGQIAYSIVDAKVRGLFMPSAYPAIEAESIRELAN
                     RLGLPAERLEATVDELNRAVRPGSFDHTTLDDCRTLGLLPDKTHWAQRLDAPPYAAYP
                     LRPGITFTYLSVRVDTAARVLATDGAPFENVYAAGEIMAGNVLRKGYIAGIGMTIGTV
                     FGRIAGEGAARHANH"
     gene            5192..6280
                     /gene="tcuB"
                     /locus_tag="VFL84_11965"
     CDS             5192..6280
                     /gene="tcuB"
                     /locus_tag="VFL84_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HET7609386.1"
                     /translation="MQTTELEREGARVMTVCNACRYCEQYCPAFQAMEQRVTFAGADL
                     NYVANLCHGCGECLYACQYAPPHELAINVPKLFAQLRVRSYEQYAWPAALGAAFRRQG
                     VWTALLLAAVMIALLLGATVALNRGALLEPGASADFYAVAPHGVMAALFGGIGVFVLV
                     ALAIGAVRCVRDQRAMNAAPPHGERGGTGAAFADILTLNHLHVAGRDCVTRLEVRTPW
                     RRWLHHATFYGFMLCFASTCVATLYHLAGAPAPYGYSSLPVLLGTAGGIGLLIGTAGA
                     LLHRRARDAALSDAAQDDLDRSFLVLLLLTAATGLLLLALRHERAMGLLLVVHLGVVL
                     ALFLTLPYGKLVHGIYRGIALLRYRRER"
     gene            complement(6321..6836)
                     /locus_tag="VFL84_11970"
     CDS             complement(6321..6836)
                     /locus_tag="VFL84_11970"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HET7609387.1"
                     /translation="MKEPGDRAADDVVLSAFIDDELAAADAASVRARLSTDTWFAHRF
                     SAMFRATAAIRGAYRDWVDVIAAVAAVHARALQDAYRSGAGDRAAAAFTERPRLRNVD
                     LAVARLTSEQRTALCLAVLEDLSYVEIAAALRVTDGTVKARLAEARTALAAKLDETWF
                     AAMLPPSTTRQ"
     gene            7014..7586
                     /locus_tag="VFL84_11975"
     CDS             7014..7586
                     /locus_tag="VFL84_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET7609388.1"
                     /translation="MSKQPPPRDAAWPAGTVFTVGHSTLPIERFLALLAAYGIEQLAD
                     VRTVPRSRRNPQFNADALRESLAGAGIAYVPLRELGGLRKPRADSPNAGWRNESFRGY
                     ADYMQTEEFGNALERLVELAAERRTAIMCAEAVPWRCHRSLVADALTVRGIGVVEILS
                     ETSYREHALTPFARVSGTTVTYPPAQDRLI"
     gene            7680..>8288
                     /locus_tag="VFL84_11980"
     CDS             7680..>8288
                     /locus_tag="VFL84_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609389.1"
                     /translation="MLSRSLARCCAACAAFAAAHCNAQSSDADPFHWPYATALGSGVY
                     GLGDGTEAQTYRANFSVSLRDALDDRAGVRLLLPVAFGVQNLDDDALPLDRGNDGIEH
                     AGFLPGVELEHLPGERWTLRSRAQLGYAKELEGTKQSARLAAFGLRSRVTFADAPGNP
                     SLINGVLWTGFDSSGGARSSLIRLTAGVEFDIRAARWRVRDSP"
BASE COUNT         1103 a         2907 c         2891 g         1387 t
ORIGIN      
        1 cggatgtcgt gcacgcgcgt gttgagctct tccgcaacgt ccctcgtcga cagcgcgaat
       61 cccggcgaga gcttcgcgtg cagctgcagg atgcgcagct cgtcgcgcga aaggcccggc
      121 tcctcggcga cgtcgatcga cggctccttg cgctgcacac gaagcttgcg ccgatagtcg
      181 gcgaacacga gcgccatgcg ccagcgccgc cgcacctctg accctgcgta gatgatgtgg
      241 cccgtgagcg cgcggtcgaa gcggcggacg ccggaggtcg ccgcaatcgc gacgatcgcg
      301 agcaagatcc agctgaacca cgcgatgaac cccgcgaacg tgccgccgta atcggcggga
      361 ctgatgccga gcgtctcggc gatcggcgcg gcgcggctcg agagcagata gatgaaccag
      421 tcgccgggaa ggaagaacag ctcttcgaac gacgggatga cgagagagcc cgtctcggcg
      481 acgaacggat tcggcggtgc agtaatcggt tccatcgtgg cgaccccgct gtctctcgcg
      541 agaccggaag ctttgcgtcc ccgcctcgcg acgggtttgc ctttggcacg gcgctagtct
      601 ccgcccatga tccgggcggc acaacgtgct ttttgacaga aggccggatc tagattccgg
      661 ctttggcgag cgtgtccatg atctggcgca aaacgccggc cagcgacgga tgctccgcct
      721 cgaaccgcac cgcgagcgcc tcgacgggct cggtggcgac ggcgctcgcg tcgtcgttct
      781 cgagcgccgt ttcgatgtcg cgcgcgacgg tcgtgagcag cttgagcgcc tcgggatcga
      841 gcgagcgcgc ctgcttgagc cgcaggtgaa gctccgcgag cagcgcctcc agatcttggt
      901 ccctcatccg atccctcgcg gcaacgaaaa cattgggctc actgtgcctc tatataatgt
      961 gcgcggcaag caagggggtt cgcatcctga gctcgaacgt gctccgcgcc gcgctgctcg
     1021 cgctcgcgat gctgacggcg tcgaatgcgc gggcgcaacc gcttttcgag gagctgcgcc
     1081 tcattgcgcc ggcggcaccc ggcggcggct gggatcagac cgcgcgcgtc atgcaacaag
     1141 cattgcagcg ggaaaggatc gtcacgacgc ccgtcgtcga gaacgtgccc ggcgccgccg
     1201 gaaccatcgg cctcgcgcgc ttcgtcggag ccgagcgcgg caacggcgac gtgctgctcg
     1261 tgtccgggct catcatgctc ggcggcatcg tgacttacga ctcgcccgtc acgctcgccg
     1321 atgccgtgcc gatcgcgcgc ctcacgggcg agtacgaggc cctcgccgtg ccggcggcgt
     1381 cgccgtttcg cacgctcgag gatcttctcg cggcgttccg caccgacccc gagtcgatct
     1441 cgtggggcgg cggctcggtc ggcggcagcg accagatcct cgcgggcctc atcgccgcgg
     1501 ccgtcggcgt cgagccgcgc cgcgtcaact acatcggcta ttccggcggc ggcgaggcgc
     1561 tcgcggccgt cctcggcggg caagtgtcgg tcggcgtcaa cggcctcgcg gaattcgcgg
     1621 cgcaggtcga cgcgggggcg ctgcgcgtgc tcgcggtgtc gagcggcgag cgcttgccgg
     1681 gcgtcgacgc gccgaccttg cgcgagcgcg gcgtcgacgt cgagctcgag aactggcgct
     1741 cggtgctcgc gccgcccgga atcacggccg gcgagcgcgc gcggctcgcg gccgcggtcg
     1801 acgcgatggt tcgctcgaac gagtggcgcg cgatgctcgc gcgttatcgc tggctcgacc
     1861 gctatctcgc aggcgacgag ttcgcggcgt tcgcggcggc cgaggagcgg cgggttcgtt
     1921 cgatcttgcg cgacctcggc gcgagcgcgg cggccgggga tgcgcgtgcg ttcgatccgt
     1981 atgcggcgtt cgttctgacg ggtctcgcgc tgttcggcgt cgcggcgagc gtctccgtgc
     2041 tgcgcgcgcg gaacagcgcg cacgagcgcg cgccgctgcc gtggcgcccg ctggcgctga
     2101 tcggcgccgg cgcggcgctg cacgtgctgc tcgcggaaac cttgggcttc atcgtcgcgg
     2161 ccgcggtgtt gttctggctc gtcgcgcggg ccttcgacac gcgccacccg gcgcgcgatc
     2221 tcggcatcgc gctcgccgtc gcggcggtca gctacgcgct cttcgactac gcgctcgatc
     2281 tgccgctgcc ggccggcgtg ctcggcgcct ggctctaggc gatggacacc ttcgaaggcc
     2341 tgagcttcgg attcgcgaac gcgctgacgc cgcagaacct tctctgggcg ctgctcggca
     2401 cgacgctcgg caccgcgatc ggcgtgctgc ccggcatcgg tccggcgctc accgtcgccg
     2461 tgctgctgcc ggtcacgttc ggcctcgatc ccgtcagcgc gttcatcatg ttcggcggca
     2521 tctactacgg cgcgatgtac ggcggctcga cgacgagcat cctgctcaac acacccggcg
     2581 agaccggggc gatcgcgacg gcgatcgacg gtcatcagat ggcgctgcgc ggccgcggcg
     2641 cggcggcgct gacgacggcg gcgctcggct cgttcgtcgc cggcacgatc gcgacgctcg
     2701 cgctcgcgct gttcgcgccg gcgctcgcgt cggtcgcgct cgagttcggg cccgccgagt
     2761 acttcgcgct cgcgctgctc gcattttcgg ccgtgacggc gctgctcggc gcgtcgctcg
     2821 cgcgcggtct tttcagtttg tttctggggc tcgcgctcgg cctcgtcggc atcgaccagc
     2881 tcacgggcca ggcgcggctc tccttcggca tcccgcagct gctcgacggg atcgacgtcg
     2941 tgatcctcgt catcgggctc ttcgccgtcg gcgagacgct gcaccaagcc gcgcggccga
     3001 gccgcgccga ggccgcgccc gtgcaactga cgcgcttcat gagcatgagc cgcgaggact
     3061 ggcgccgctc gtggaaggca tggctgcggg gcactgcgat cggcttcccg ctcggcatcc
     3121 tgccgatggg cggctcggtg atcccgacgt tcctgtcgta ctacgccgag aagcgcttga
     3181 gcccgaagcc ttcggagttc ggccacggcg cgatcgaagg cgtcgccggg cccgaggccg
     3241 cgaacaacgc gtcgacggcc ggcgtgctcg tgccgctttt gacgctcggc gtgccgtcgt
     3301 cggcgacggc ggcgatcttg ctcgcggcgt ttcagcagta cgggctgcag ccggggccgc
     3361 tgctgttctc gacacgcccg gatctcgtct ggacgctgat cgcgagcttg gtcttgggca
     3421 acgtgatgct gctcgtgctg aacctgccgc tcgtgagcgt ctgggcgcgc gtcatgctgc
     3481 tgccgcggcc gctcctgttc ggcgtcgtgc tcgtgctcgc gtcgctcggc gtctacagct
     3541 tgaatcgctc gctgctcgac gtaggccttc tctacgccgt cggcgcgctc ggtctcgcgc
     3601 tgcgcgtgct cgacgtgccg ctcgtgccgg ccgtgctcgg gctcgtgctc gggccgatgg
     3661 ccgagcagca attccgccgc gccgtcgcga tcagcgaagg cgatctcacg gtgttcgtga
     3721 cgcggccgat ttccgcggcg ctgctcacgg ccgcggcgct ggcgctcttg gcgccggcgc
     3781 tgctcaaacg tcggcggcgc cgtcgacatg agtgacaccg gcgcacctta cgacgtcgtc
     3841 gtcatcggcg gcggcaacgc gggcctgtcg gccgcgctga cggcacgccg cgccggcgcg
     3901 cgagtgttgg tgctcgagcg cgcgccgcgc gagctacgcg gcggcaatag ccgccacacg
     3961 cgcaatctgc gctgcgctca cgccgcgccg acggacatct tgaccggctc gtatccgggc
     4021 gaagagctgc tcgccgatct cgcgcgcatc aacgacggcg agaccgacga ggcgctcgcg
     4081 gcgctcgtcg cggcgcgctc gagcgactgc gtgccgtgga tggcggcatt cggcgcgcgg
     4141 ttccaagcgg cgctgcgcgg cacactgcaa ttgtcgcgca cgaacgcgtt tttcctcggc
     4201 ggcggcacgg cgctcatgaa cagctactac gccgcggcag agaagctcgg catcgacgtg
     4261 cgctacgacg ccgaagtcat cgagctcgtg cttcgcgacg gcaagttcga gtctgcgctc
     4321 gtgcggtcga gaggtgccga gcaagccgtc cgcgcgcgcg ccgtcgtcgt cgcggccggc
     4381 ggcttcgaag cgaacctcga gtggctgcgc gacgcatggg gcgacgcggc gcgcaacttc
     4441 ctgatccgcg gcacgccgta taacacgggc acgatgctgc gcgcattgct cgacgccggt
     4501 gcggcgccga tcggcagtcc cagcgaatgc cacgcggtcg cgatcgacgg ccgcgcgccg
     4561 aagttcgacg gcggcatcgt gacgcggctc gactgcctgc cgctcggcat cgtcgtcgat
     4621 cgcgatgcgc agcggttcta cgacgagggc gaggacgtct ggccgaagcg ctacgcgatc
     4681 tggggcaagc tcgtcgcgcg gcggccggga cagatcgcct actcgatcgt cgacgcgaaa
     4741 gtgcgcggct tgttcatgcc ctcggcatat cctgcgatcg aggcggagtc gattcgcgag
     4801 ctcgcgaatc ggctcggctt gccggccgaa cggctcgagg cgaccgtcga cgagctcaat
     4861 cgcgccgtac ggccgggctc gttcgatcac acgacgctcg acgactgccg caccttaggg
     4921 cttttgccgg acaagacgca ctgggcgcag cggctcgacg caccgccgta cgcggcgtat
     4981 ccgcttcggc ccggcatcac gttcacgtac ttgagcgtgc gcgtcgatac cgcggcgcgc
     5041 gtgctcgcga cggacggcgc gcctttcgag aacgtctatg cagccggcga gatcatggcc
     5101 ggcaacgtgc tgcgcaaggg ctatattgcc ggcatcggca tgacgatcgg caccgtgttc
     5161 ggccgaattg cgggcgaggg cgcagcgcga catgcaaacc actgagcttg agcgcgaagg
     5221 cgcgcgcgtg atgaccgtct gcaacgcctg ccgctactgc gagcagtact gccccgcgtt
     5281 ccaagcgatg gagcagcgcg tgacgttcgc cggcgcggat ttgaattacg tcgcgaacct
     5341 gtgccacggc tgcggcgaat gcttgtacgc gtgccagtac gcgccgccgc acgagctcgc
     5401 gatcaacgtg ccgaagctgt tcgcgcagct gcgcgtgcgc tcgtacgagc agtacgcgtg
     5461 gcccgccgcg ctcggcgcgg cgtttcgccg ccaaggggta tggacggcgc tgctgctcgc
     5521 ggcggtcatg attgcattgc tgctcggcgc gacggtcgcg ctgaaccgcg gcgcgctgct
     5581 agagccgggc gctagcgccg acttctacgc ggtcgcgccg cacggcgtca tggccgcgct
     5641 gttcggcggc atcggtgttt tcgttctcgt cgcgttggca atcggcgccg tacgctgcgt
     5701 gcgcgaccag cgcgcgatga acgcggcgcc gccgcacggc gagcgaggcg gcacgggcgc
     5761 cgcatttgcc gacatcttga cgctgaacca tctgcacgtt gccggccgcg actgcgtcac
     5821 gcggctcgag gtccgcacgc cgtggcgccg ctggctgcat cacgcgacgt tctacggctt
     5881 catgctgtgt ttcgcctcga cctgcgtagc gacgctctat cacctcgccg gcgcgcccgc
     5941 gccttacggt tattcgagct tgcccgtgct gctcggaacc gccggcggta tcgggctctt
     6001 gatcggcact gccggagcgc tgttgcatcg ccgcgcacgc gacgccgcgc tctcggatgc
     6061 ggcgcaagac gaccttgacc gatccttctt ggtgctgctg ttgctgacgg ccgcgacggg
     6121 cttgctgttg ctcgcgctgc gccacgagcg cgccatggga ctgctgctcg tcgtgcactt
     6181 gggcgtcgtg ctcgcgctgt tcctcacgct gccgtacggg aagctggtcc acggcattta
     6241 tcgcggcatc gcgctgctgc ggtaccgacg cgagcggtag tgctttccct cgacgtagcc
     6301 tcgtcgaaaa agaccgccgt tcactgccgc gtcgtcgacg gcggcagcat cgccgcgaac
     6361 caggtttcgt cgagctttgc ggcgagcgcc gtgcgcgcct cggccagtct cgctttgacc
     6421 gtgccgtcgg tcacgcgcaa cgccgccgca atttcgacgt agctcaggtc ctctagcact
     6481 gcgaggcata gcgcggtccg ctgctcggac gtcaagcgtg cgacggcgag gtcgacgttg
     6541 cgcaaccgcg ggcgctcggt gaacgcagcg gcagcgcgat cgccggcccc gctgcggtaa
     6601 gcgtcctgga gcgcgcgcgc atgaacggct gctacagcag cgatgacgtc gacccaatcg
     6661 cgatacgccc cgcgaatcgc agcggttgcc cggaacatcg cgctgaaccg atgcgcgaac
     6721 caggtatcgg tcgaaagcct cgcgcgcacg cttgcggcat ccgccgcggc cagctcgtcg
     6781 tcgatgaacg ccgagagcac cacgtcgtcg gcagcccggt ctcctggctc tttcatcgcc
     6841 gtattcccct tcacgccgcc cccttcgctg tgcctctgtg gagacaacgt tcgagcgcgc
     6901 ggagttattc cgcggcgcaa gcgacggttc aggggggtgc gcccgaatcc ccggggttgc
     6961 cgtggtattt ttggtgtgcg acgcgatcta aacgtgtctg ccaaggaggc gccatgagca
     7021 agcaaccgcc gccgcgggac gcggcctggc ccgccggaac ggttttcacc gtcggccact
     7081 cgacgctgcc gatcgagcgc tttctcgcgc tgctcgccgc atacggcatc gagcagctcg
     7141 ccgatgtccg gaccgtgccg cgctcgcggc gcaatccgca gttcaacgcc gacgcgctgc
     7201 gcgagtcgct cgccggggcc ggcattgcct acgtgccgct tcgcgagctc gggggcctac
     7261 gcaagccgcg cgccgattcg ccgaacgccg gctggcgcaa cgaaagcttc cgcggctatg
     7321 ccgactacat gcagaccgag gagttcggca acgctctcga gcgtctcgtc gagctggcgg
     7381 cggagcggcg cacggccatc atgtgcgccg aggcggtgcc gtggcgctgc catcgttcgc
     7441 tcgtggccga cgcgctcacg gtgcgcggca tcggcgtcgt cgagatcttg agcgagacga
     7501 gctaccgcga gcacgcgctg acgccgttcg cacgcgtgag cggcaccacg gtcacctatc
     7561 ctccggcgca agaccggctc atttgagcgc gccgcgccgc gctggcgcgg aacttgctgc
     7621 aatcgcgccg tcgacgcggc caacggatgg ccgcgcgcca acagccaagg atggccggca
     7681 tgctctcacg ctcgctcgcg cgctgttgcg ccgcgtgcgc cgctttcgcg gccgcgcatt
     7741 gcaacgcgca gtcttccgat gccgaccctt ttcattggcc ttacgcgacc gcgctcggct
     7801 cgggtgtcta cgggctcggc gacggcacgg aggcgcagac gtatcgcgcg aacttctcgg
     7861 tgagcctgcg cgacgcgctc gacgaccgcg ccggcgtgcg gttgctgctg cccgtcgcgt
     7921 tcggcgtgca gaatctcgac gacgatgcac tgccgctcga ccgcggcaac gacggcatcg
     7981 agcacgccgg attcttgccc ggcgtcgagc tcgagcacct gcccggcgag cgctggacgc
     8041 tgcgctcgcg cgcgcagctc ggctacgcga aggagctcga aggcacgaag caatcggcgc
     8101 ggctcgccgc tttcggcttg cggagccgcg tcactttcgc cgacgcgccg ggaaatccct
     8161 cgctgatcaa cggcgttctc tggacggggt tcgattcgag cggcggcgcg cgcagctcct
     8221 tgatacgcct cacggccggc gtcgaattcg acatccgcgc cgcgcgctgg cgcgtgcgcg
     8281 acagcccg
//
LOCUS       DASSNN010000642         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_485942, whole genome shotgun sequence.
ACCESSION   DASSNN010000642 DASSNN010000000
VERSION     DASSNN010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485942"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..906)
                     /locus_tag="VFL84_11985"
     CDS             complement(181..906)
                     /locus_tag="VFL84_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7609390.1"
                     /translation="MRTQARGGAAPGAVADAAPVLEAHGLVKTFSDVGRNVEVLRGVE
                     LSVAKGDRVAIIGTSGSGKTTLLQLIGGLDQPTSGEVRVAGESMSALRDADRGRLRNR
                     ALGFVYQFHHLLPEFTALENVAMPLLVRGMAPAQAAAEARGLLERVGLSERLEHRPSQ
                     LSGGERQRTAVARALVTRPAAVRADEPTGNRDRPTGLRVFELLLELNAALGTSLVVVT
                     HDPDLAARMDRVLELKDGVLRPV"
     gene            complement(908..2170)
                     /locus_tag="VFL84_11990"
     CDS             complement(908..2170)
                     /locus_tag="VFL84_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HET7609391.1"
                     /translation="MRQPYELSIAVRYLRTRSAHSFISFISAVSMIGIALAVAVLIVV
                     LSVMNGFENELQQRILGMVSDAQIIGYPGRFTDWREARERVLDRDDVEAVAPYVEGLA
                     MAVAGEALAGVTVRGVEPTLETTVSTIDEVMTSGDFGSLTPGSFNVVIGAQLAKLLGV
                     GIGDEVGVWLAEGRMTLAGFLPRHRTFTVSGIFDAGMYEYDRGLVFVSFDDAAKLFGT
                     DGEATGLRLAIPDLYNASDVAKQAAQQLIEEGFAQKYQYDNWTSVHYTFFRSIQLQKT
                     MLFVILSLVVAVAAFNIVSTLMMVVRDKRGDIAILRSVGTTQGSVMAMFASQGTLIGF
                     IGTGLGVLLALLFCWQIGNIVDLLQWLFGVDLLSAEVYYLDTLPAQVRPLEVLQIGGL
                     ALALAVAATFYPALAAARQPPAEALRYE"
     gene            complement(2175..>2540)
                     /locus_tag="VFL84_11995"
     CDS             complement(2175..>2540)
                     /locus_tag="VFL84_11995"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7609392.1"
                     /translation="VVIDKQKDIAILRTYGLEAGRVARIFIVQGAVIGLVGALLGAGL
                     GLLLAYNIGVVVPWLEQTFGFKIMPGDVYYVTELPSEVQLFDVVAVPVAAFVLAILAT
                     LYPARRAAAVAPALALRYD"
BASE COUNT          457 a          870 c          843 g          370 t
ORIGIN      
        1 cgcccacgag cagcggccga agagccgcgc agcgcagcgc cgcgagcgcg gcgatcagta
       61 gcggcacgct ccatgctgcg ctcggcaact gcggcaaccg atgcgccgcg agcgtgccga
      121 gcagccacgc gccggcaatc cagacgatcg gcgccgggcg aaccgtccgc gcgattttgg
      181 ctagacgggc ctcaatacgc cgtccttgag ctcgagcacg cgatccatgc gcgccgccag
      241 atccggatcg tgagtgacga cgaccaggct cgtgccgagc gccgcgttga gctcgagcag
      301 aagctcgaag acgcgcaagc ccgtcggccg atcgcggttg ccggtcggct cgtcggcccg
      361 caccgcggcc ggccgcgtca cgagcgcgcg cgcgacggcc gttcgctgcc gttcgccgcc
      421 cgaaagctgg ctcggccgat gctcgagacg ctcgctcaag cccacgcgct cgagcaggcc
      481 gcgcgcttcg gcggccgctt gcgcgggcgc catcccgcgc acgagcaacg gcatcgcgac
      541 gttctcgagc gccgtgaact ccggcagcag atggtgaaac tggtagacga agccgagcgc
      601 gcgattgcgc aaccggccgc gatccgcgtc gcgcagcgcg ctcatcgact cgccggccac
      661 gcgtacttcg ccggacgtcg gttgatcgag accgccgatg agctgcagca gcgtcgtctt
      721 gcctgagccg gacgtgccga tgatcgcgac gcggtcgccc ttcgcgacgc tgagctcgac
      781 gccgcgcagc acctcgacgt tgcggccgac gtcgctgaac gtcttgacga gaccgtgcgc
      841 ctcgagaacc ggcgccgcat cggccactgc ccccggagcc gccccacccc gcgcttgtgt
      901 gcgcacgtca ttcatagcgc agcgcctcgg ccggcggttg gcgcgcagcg gccagtgccg
      961 gatagaacgt cgcggccacg gccagcgcga gcgccaagcc gccgatttgc aggacctcga
     1021 gcggccggac ttgcgcgggc agcgtgtcga ggtagtagac ctccgccgat aggagatcga
     1081 cgccgaacag ccactgcaga agatcgacga tgttgccgat ttgccagcag aagagcagcg
     1141 ctagcaagac gccgagcccc gtgccgatga agccgatcag cgtgccctgg ctcgcgaaca
     1201 tcgccatgac gctaccttgc gtcgtgccga cgctgcgcag aatcgcgatg tcgccgcgct
     1261 tgtcgcgcac gaccatcatc agcgtcgaga cgatgttgaa tgccgccacg gcgacgacga
     1321 gcgacaggat cacgaacagc atcgtcttct gcagctggat cgagcggaag aacgtgtaat
     1381 gaacgctcgt ccagttgtcg tactgatact tctgcgcgaa accttcctcg atgagttgct
     1441 gtgcggcctg ctttgcgacg tcggacgcgt tgtagaggtc gggaatcgcg agccgcaggc
     1501 ccgtcgcttc gccgtccgtg ccgaacagct tcgctgcgtc gtcgaagctc acgaacacga
     1561 gcccgcgatc gtactcgtac atgccggcat cgaagatgcc ggatacggtg aaggttcgat
     1621 gacgcggcag gaagccggcg agggtcattc gcccctccgc gagccagaca ccgacctcat
     1681 cgccgattcc gacgccgagc agcttcgcga gctgcgctcc gatcacgacg ttgaagcttc
     1741 ccggcgtcag cgagccgaag tcgccgctcg tcatgacctc atcgatcgtc gagacggtag
     1801 tctcgagggt cggctcgacg ccgcgcacgg tgacgcccgc gagcgcctcc cccgccacgg
     1861 ccatcgccaa gccctcgacg taaggcgcga cggcctcgac gtcgtcgcgg tcgagcacgc
     1921 gctcgcgcgc ttcacgccag tccgtgaagc ggccggggta accgatgatt tgtgcgtcgg
     1981 agaccatccc caagatccgt tgttgcagct cgttctcgaa accgttcatg acggagagca
     2041 cgacaatcaa taccgcgacc gcgagcgcaa tgccgatcat cgaaactgcc gagatgaacg
     2101 agataaagct gtgcgcgctt cgggtgcgca gataacgcac ggcgatcgag agctcgtagg
     2161 gttggcgcat gcggctagtc gtagcgcagc gcgagcgcgg gtgcgacagc tgccgcgcgg
     2221 cgggcagggt acagcgtcgc gaggattgcg agcacgaacg ccgccacggg cacggcgacg
     2281 acgtcgaaga gctgcacttc cgacgggagc tccgtcacgt aatagacgtc gccgggcatg
     2341 atcttgaagc cgaaggtctg ttcgagccac ggcacgacga cgccgatgtt gtaggcgagc
     2401 aagagaccca agcccgcgcc gagcagcgcg ccgacgaggc cgatgacggc gccttgcacg
     2461 atgaagatgc gcgcgacgcg gcccgcctcg agaccgtagg tgcgcaggat cgcgatgtcc
     2521 ttctgcttgt cgatcaccac
//
LOCUS       DASSNN010000643         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_850049, whole genome shotgun sequence.
ACCESSION   DASSNN010000643 DASSNN010000000
VERSION     DASSNN010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850049"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VFL84_12000"
     CDS             complement(<1..440)
                     /locus_tag="VFL84_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET7609393.1"
                     /translation="MEYKDYYQIMGVGRNASADEIKRAYRKLARKYHPDVSKEKDAEA
                     RFKEVGEAYEVLRDPEKRAAYDALGSRKPGEEFRPPPDWQFDYGAAESDLSGHSDFFE
                     QLFGGLGRGRRGSFRSRGFDTAGQVEVTLEEAFHGTTRTLEMLST"
     gene            544..1944
                     /locus_tag="VFL84_12005"
     CDS             544..1944
                     /locus_tag="VFL84_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET7609394.1"
                     /translation="MQAGQLLATALAATGFAAGTVASLHALHFKRRPQSAFAWIAVCM
                     TLPLFGALLYFLFGINRVQTRARKLLTRHPEPDCPTEYVGTPPPQLQPLARLGSAVSG
                     WPLTAGNRVELLHEAAAIFDAMIAAIERAREYVYFSSYIFDGGPLGQRFVAALTAAAD
                     RGADVRVLLDGFGEWYSLNPARKLLEGTRVRFARFLPPRLLPPSFSVNLRNHRKILIV
                     DGLEAFTGGINIRDRYLDTGHLRIIDSHFRLSGPVLAQIETVFLRDWQFVTGDPGIVA
                     RRVPQPTGGALCRVIADGPAAGFDRLTALLTGAIASARRRVAIMTPYFVPPRELISPL
                     QAAALAGVDVAVILPERNNLPYVHRATRHMLWELLERGVHVYYQPPPFVHSKLFYVDD
                     YYAQVGSANFDSRSLRLNFEMNVEVYDRDTVTELATHFEAIRERSRRVTLAEVDARPF
                     LTRTIDGLAWLFSPYL"
BASE COUNT          304 a          755 c          692 g          379 t
ORIGIN      
        1 gtcgaaagca tctcgagcgt gcgcgtcgtg ccgtggaacg cctcctcgag cgtgacctcg
       61 acctgcccgg ccgtgtcgaa cccgcggctt ctgaacgagc cgcgccggcc gcggccgagg
      121 ccgccgaaca gctgctcgaa gaaatcgctg tggcccgaaa gatccgattc ggcggcgccg
      181 tagtcgaact gccagtccgg cggcgggcgg aactcttcgc cgggcttgcg cgagcctagc
      241 gcgtcgtacg ccgcccgctt ctcgggatcg cgcagcacct cgtaggcctc gccgacttcc
      301 ttgaacctgg cctcggcatc tttttccttg ctgacgtcgg ggtggtactt gcgcgcgagc
      361 ttccggtagg cgcgcttgat ctcgtcggcc gacgcatttc ggccgacgcc catgatctgg
      421 tagtagtcct tgtattccac gccgcccttc cgtgtagaag cgaactcccg tagtggcggc
      481 gcgacgcatc gctttcaacg gcctcgctgg ccgcgcctcg acccatatgc tacacatcgc
      541 gccatgcagg caggccagct tttggcgacc gctcttgccg ccaccggctt cgccgccggc
      601 accgttgcgt cgctgcacgc cctgcacttc aagcggcggc cgcaatcggc cttcgcctgg
      661 atcgccgtct gcatgacgct gccgctattc ggcgcgctcc tctatttctt gttcggcatc
      721 aaccgcgtgc aaacgcgggc ccgcaagctc ttgacgcggc atcccgagcc cgactgcccg
      781 accgagtatg tcggcacccc gccgccgcag ctccagccgc tcgcgcggct cggctcggcc
      841 gtcagcggct ggccgctgac ggccggcaat cgcgtcgagc tgctgcacga ggcggccgcg
      901 atcttcgacg ccatgatcgc ggcaatcgag cgggcgcgcg agtacgtcta cttcagcagc
      961 tacatcttcg acggcggccc gctcgggcag cggttcgtcg cggcgctcac ggcggccgcg
     1021 gaccgcggcg ccgacgtccg cgtgctgctc gacggcttcg gcgagtggta ctcgctgaac
     1081 ccggcgagga agctgctcga gggcacgcgc gtgcgctttg cgcggttcct gccgccgcgg
     1141 ctgctcccgc cgtcgttcag cgtcaacctg cgcaatcacc gcaagatcct gatcgtcgac
     1201 ggcctcgagg cgttcacggg cggaatcaac atccgcgacc gctatctcga tacggggcac
     1261 ttgcgcatca tcgactcgca ctttcggctg agcggcccgg tgctcgcgca gatcgaaacg
     1321 gtgttcttgc gcgattggca gttcgtgacg ggagatcccg gcatcgtcgc gcggcgcgtg
     1381 ccgcagccga ccggaggcgc gctgtgccgc gtcatcgcgg acggcccggc cgcgggattc
     1441 gaccggctca cggcgctttt gaccggcgcc atcgcgagcg cgcggcggcg tgtcgcgatc
     1501 atgacgccgt acttcgtgcc gccgcgcgag ctgatctcgc cgctccaggc ggccgcgctc
     1561 gcgggcgtcg acgtggccgt catcctgccc gagcgcaaca acctgccgta cgtgcatcgc
     1621 gccacgcgcc acatgctctg ggagctgctc gagcgcggcg tccacgtcta ttaccagccg
     1681 ccgccgttcg tgcacagcaa gctgttctac gtcgacgact attacgcgca ggtcggctct
     1741 gcaaatttcg attcgcgcag cctgcggctg aacttcgaga tgaacgtcga ggtatacgac
     1801 cgcgataccg tcacggagct cgcgacgcac ttcgaggcga tccgcgagcg atcgcgccgc
     1861 gtgacgctgg ccgaggtcga cgcgcggcca ttcctgacga ggacgatcga cggcctcgcc
     1921 tggcttttct caccgtacct ttgaggagtc gccgcgatgc agcacagtgt tacggcggcc
     1981 gcgcggaaac gcgggccgat gctctccacg ctactcgcgc gttggcggaa cggcgatacg
     2041 cacatttacg tacgcacgca aatggcctga gcgcgcaaca ttgatgggcg tgtgcgagaa
     2101 agagctgcgg ctggcgttgg tttgcttcgg
//
LOCUS       DASSNN010000644         4850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1493563, whole genome shotgun sequence.
ACCESSION   DASSNN010000644 DASSNN010000000
VERSION     DASSNN010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493563"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VFL84_12010"
     CDS             <1..856
                     /locus_tag="VFL84_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609395.1"
                     /translation="SWGDPAIAGAYNNSDETGIPFERPDAFAGRTLESFTQEELAAIT
                     AERQKQTIERNPTLSEFPGATSPMHWFENYFAANSRAWLVADPPDGKVPPQTEEARQR
                     AARAGRGPADSYLDRSLYDRCITRGLPGSMMPAIYGNSYQIAQSPDAVTITYEMIHDT
                     RVIPLDGSPHVSPAIRQYLGDARGRWEGNTLVVETTNFNGLASFRGSSEHLKLVERFT
                     PLGRDTLEWSVTFDDPHTWERPWTFAMNLVHDESQPLFEYACHEGNYGLANILSAARA
                     EEAAAKKK"
     gene            897..1934
                     /locus_tag="VFL84_12015"
     CDS             897..1934
                     /locus_tag="VFL84_12015"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7609396.1"
                     /translation="MNELRSRTVTLASLRSALCAAALLALGAASVAQAQPAGQIKSLS
                     HNIHAVNDLDTTLSFYKEVFGLDGRATDFANPAVPLLTNAPGVTLRMSMLSLPGGPRY
                     ELTHFKGLERQPGVAKYTDPGAASVVLYVRDLDAVVAAAKKAKAQLVTTGGEPIHVAT
                     ASGHARSIVLRDPDGYFLQLVEEAPPAGAPAGTIHRATLAFTMEDAAATQRFYTGMMA
                     VPLSGPTEFKRDASLAKLYGAPETVEIRKLTGTLPPGTAVEFTEFRGVPRTKFHLRVR
                     DPGAPAMAINVDNLEGMVAQMKAAGVNLISANGELVDFGNGVRNIFVEDPNGLNLELI
                     YRPAAPARPAN"
     gene            1945..2778
                     /locus_tag="VFL84_12020"
     CDS             1945..2778
                     /locus_tag="VFL84_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7609397.1"
                     /translation="MHRNRTFAAAAALTLVSIGSLGFSVWAQSGAPSTGPSAAPRPRA
                     QGYLGADAVPDHRVFLPPPPAVDSPAGIADVAIFHATRSLEGSARWQLAASDDRLGAR
                     KMLGDFGCAMGVDLAAVEAPALERTLARAGADLFPMVGQAKDHYRRPRPFVTESGPIC
                     VAVSPDFAASGSYPSGHSASAWLYTLLLAEIDPDNAAAIVARGRAFGESRVVCGVHYE
                     TDVEGGRLTTTALVAALHGDAEFQADMAAARAELAKARAAAPKPDAASCPADAALATP
                     W"
     gene            complement(2792..3973)
                     /locus_tag="VFL84_12025"
     CDS             complement(2792..3973)
                     /locus_tag="VFL84_12025"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7609398.1"
                     /translation="MKRLTSTLLAAGLALGAAAPLAAQDLTITNAHIVVGNGQVIERG
                     SIVVRGGKIESVAAGAPARTVGQVIDAKGMSAMAGFIDAHRHVNTGPEEKAQMQRQLE
                     AGYTTILSGGGPGDGNKTLRDHIESGQINGPRIIPSERIRLAQLTPDQAREAVRAMKA
                     AGIDYTGEISLTPVPGPTPHELEVLRAVLDEGNKIGVKVQVHAVSSPAMNAAVEMGIP
                     LLVHLPNKDWVSKADAEKLAAAGTKILMTIGFGAPTIDVFAKDNTPRFRDGKPWPDGI
                     VDGVGGGVEAGYAMVNARTIFDAGAIIGYCTDTNYDALAGLEHELKSMNVVFSPADMV
                     KIMGPNTAAYLGMEDQLGTLEPGKLADIILLDGDPLEGYWNWLKTKVTIKGGKVVVDV
                     R"
     gene            4190..>4850
                     /locus_tag="VFL84_12030"
     CDS             4190..>4850
                     /locus_tag="VFL84_12030"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HET7609399.1"
                     /translation="MLANPRLQALRTVWLSDIHLGSRECRVNLLLDFLRHTRCDVLYL
                     VGDIVDLENLRKSFYWPSSHTEVLRLLLKKSQEGTRIVYIPGNHDHELHAFAGVKFGG
                     IEIANHAIHTTRAGRRLLVTHGDQFDAVVRRPSLGVWLGGFACRRLLTLNRFVHWLHD
                     VFDRPYWSLAQHLKARFPGAKGYVERYQNATLAAAREARVDGVVCGHIHRAAFTEVDG
                     LL"
BASE COUNT          748 a         1718 c         1593 g          791 t
ORIGIN      
        1 gtcgtggggc gaccccgcga tcgccggcgc ctacaacaac agcgacgaga cgggcatccc
       61 gttcgagcgc cccgacgcct ttgcgggacg tacgctcgag agcttcacac aagaggagct
      121 cgccgcgatc acggccgagc gccagaaaca gacgatcgag cgtaacccga cgctcagcga
      181 gttcccgggg gccacgagcc ccatgcactg gttcgagaac tacttcgccg ccaacagccg
      241 ggcatggctc gtagccgatc cgcccgacgg caaagtgccg ccgcagaccg aggaagctcg
      301 gcagcgcgcc gcgcgggctg ggcgcggccc cgcggactcc tacctcgacc gcagcctcta
      361 cgaccgctgc atcacgcgcg gcctgccggg ctcgatgatg ccggcgatct acggcaactc
      421 gtaccagatc gcgcagagcc cggatgccgt gacgatcacc tacgagatga tccacgacac
      481 gcgcgtcatc ccgctcgacg gcagcccgca cgtgagcccc gcgatccgcc aatacttggg
      541 cgacgcgcgc ggccgctggg aaggcaacac gctcgtcgtc gagaccacga acttcaacgg
      601 cctggcgtcg tttcgcggct cgagcgagca cctgaagctc gtcgagcggt tcacgccgct
      661 cgggcgcgac acgctcgagt ggtcggtcac gttcgacgat ccgcacacgt gggagcggcc
      721 gtggacattc gcgatgaacc tcgtccacga cgagtcgcaa ccgctgttcg agtacgcgtg
      781 ccacgaaggc aactacgggc tcgcgaatat tttgtcggca gcgcgcgccg aagaggctgc
      841 tgcgaaaaag aagtagctcg gcacgtacgg ccgcaaccat tgaagggggg attccaatga
      901 acgagcttcg ctctcgcacc gtcacgctcg cgtcgcttcg gtcggcgcta tgcgccgccg
      961 cgttgctcgc gctcggcgcg gcatcggtcg cgcaggcgca gccggcgggg caaatcaaat
     1021 cgctgtcgca caacattcac gcggtcaacg acctcgacac gacgctgtcc ttctacaagg
     1081 aggtgttcgg cctcgatggc cgagccacgg acttcgcgaa tcccgccgtg ccgctgctga
     1141 cgaatgcgcc cggcgtcacg ctgcgcatgt cgatgctgtc gctgccaggc ggcccgcgct
     1201 acgagctcac gcatttcaaa gggctcgagc gccagcccgg cgtcgccaag tacacggatc
     1261 cgggtgcggc cagcgtcgtg ctctacgtgc gcgacctcga cgccgttgtg gcggctgcca
     1321 agaaagcgaa ggcgcagctc gtcacgacgg gcggcgagcc gatccatgtc gcgacggcga
     1381 gcggccacgc gcgctcgatc gtgctgcgtg acccggacgg ctacttcttg cagctcgtcg
     1441 aggaagcgcc gccggccggt gcgccggcag gcacgatcca ccgcgcaacg ctcgctttca
     1501 cgatggaaga cgccgccgcc acgcagcggt tttacacggg catgatggcc gtgccgctct
     1561 cgggcccgac ggagttcaag cgcgacgcgt cgctcgcgaa gctgtacggc gcgcccgaga
     1621 ccgtcgaaat tcgcaagctc acgggcacgt tgccgcccgg cacggccgtc gagttcacgg
     1681 agtttcgcgg cgtgccgcgc acgaagttcc atttgcgcgt gcgcgacccc ggcgcgccgg
     1741 ccatggcgat caacgtcgac aatctcgagg gcatggtcgc gcagatgaag gccgccggcg
     1801 tcaacttgat ttcggccaac ggcgagctcg tcgacttcgg caacggcgtc cgcaacatct
     1861 tcgtcgagga tccgaacggc ctgaatctcg agctcattta tcggccggcc gcaccggctc
     1921 ggcccgccaa ctaggaagaa gaacatgcac cgcaatcgta cgtttgccgc cgcagcggca
     1981 ctgacactgg tctcgatcgg ttcgctcgga ttcagcgtgt gggcgcagtc cggcgctccg
     2041 tcgaccgggc cgagcgccgc gccgcgccct cgggcccagg gctacctcgg cgccgacgcc
     2101 gtgccggacc accgcgtatt ccttccgccg ccgccggcgg tcgattcgcc cgctgggatc
     2161 gccgacgtcg cgatcttcca cgcgacacgc tcgctcgaag gcagcgcgcg ctggcagctc
     2221 gcggcgagcg acgatcggct cggagcgcgc aaaatgctcg gcgacttcgg ttgcgcgatg
     2281 ggcgtcgatc tggcagccgt cgaagcgccc gcgctcgagc gaacgctggc tcgcgcgggc
     2341 gcggacctgt tcccaatggt gggccaagcc aaggaccact accggcggcc tcgccccttc
     2401 gtcacggaat caggccccat ctgcgtcgct gtctcgcccg acttcgccgc gagcgggtcg
     2461 tacccgtccg gccattccgc ctcggcgtgg ctatacacgt tgctgctcgc ggagatcgat
     2521 cccgacaatg ccgcggcgat cgtcgcgcgt ggccgcgcat tcggcgagag ccgcgtcgtc
     2581 tgcggcgtgc actacgagac cgacgtcgag ggaggccgcc tcacgacgac ggcgctcgtc
     2641 gccgcgctgc acggcgatgc cgagttccag gccgacatgg cggccgcgcg ggcggagctc
     2701 gcgaaggcac gcgcggcggc tccgaagccg gatgccgcga gctgcccggc ggacgcggcg
     2761 ttggccacgc cctggtgaag cgcggctcgg cttagcgcac gtcgacgacg accttgccgc
     2821 ccttgatcgt gaccttcgtc ttgagccagt tccagtagcc ttcgagcggg tcgccgtcga
     2881 gcaggatgat gtcggcgagc ttgccgggct cgagcgtgcc gagctggtct tccatgccga
     2941 ggtacgcggc cgtgttcggc cccatgatct tgaccatgtc ggccggcgag aacacgacgt
     3001 tcatcgactt cagctcgtgc tcgaggccgg cgagcgcgtc gtaattcgta tcggtgcagt
     3061 agccgatgat ggcgcccgcg tcgaagatcg tgcgcgcgtt gaccatcgcg tagccggcct
     3121 cgacgccgcc gccgacaccg tcgacgatgc cgtcgggcca cggcttgccg tcgcggaagc
     3181 gcggcgtgtt gtccttcgcg aacacgtcga tcgtcggcgc gccgaagccg atcgtcatca
     3241 ggatcttcgt gccggccgcg gccagcttct cggcgtcggc cttgctgacc cagtccttgt
     3301 tcggcaagtg cacgagcagc gggatgccca tctcgacggc cgcgttcatc gcgggcgagc
     3361 tcacggcgtg gacttgcacc ttgacgccga tcttgttgcc ttcgtcgagc acggcgcgca
     3421 gcacctcgag ctcgtgcggc gtcgggcccg gcaccggcgt cagcgagatc tcgcccgtgt
     3481 agtcgatgcc ggcggccttc atcgcgcgca cggcctcgcg cgcctggtcc ggcgtgagct
     3541 gcgcgaggcg gatccgttcg gagggaatga ttcgcgggcc gttgatctgc cccgactcga
     3601 tgtgatcgcg cagcgtcttg ttgccgtccc cggggccgcc gcccgagagg atcgtcgtgt
     3661 agccggcctc gagctggcgc tgcatctgcg ccttttcctc ggggcccgtg ttgacgtgcc
     3721 ggtgcgcgtc gatgaacccc gccatcgcgc tcatgccctt cgcgtcgatg acctggccaa
     3781 ccgtgcgcgc cggcgcaccc gcggccaccg attcgatctt gccgccgcgc acgacgatcg
     3841 agccgcgctc gatgacttgg ccgttgccga cgacgatgtg cgcgttcgtg atcgtcaagt
     3901 cctgagccgc gagcggcgca gcggcgccga gcgcgaggcc cgcggccagc agcgtcgagg
     3961 tcaatctttt catgtgtttt cccccttcaa tgggctcatc ttaacggcgc cgcgtggtcg
     4021 cggggcccat tcgagccggc attgaccgtt cttaagacct cggtcagcag gccggagcaa
     4081 gctcttgggg gcaggcggcg tctccaatcg gcgacgattt ctgcgatcca cgtcgaagca
     4141 aatagactcg cgagccttca ctcttggccc tctcatggag gagagaccgt tgctagcgaa
     4201 tccgcgcctt caagccctcc gtactgtctg gctctccgac atccacctgg gctcccgcga
     4261 atgccgagtc aatctgctgc tcgacttctt gcgccatacg cgctgtgacg tcctctatct
     4321 cgtcggcgac atcgtcgatc tcgagaactt gcgcaagagc ttctactggc cgtcgagcca
     4381 cacggaggtc ctgcgcctgc tgctgaagaa gagccaggaa ggcacgcgga tcgtctatat
     4441 ccccggcaat cacgaccacg agctgcacgc cttcgccggc gtcaagttcg gcggcatcga
     4501 gatcgcgaac cacgcaatcc atacgactcg cgccggccgc cgcctgctcg tcacgcacgg
     4561 cgaccagttc gatgcggtcg tgcgccggcc ctcgctcggc gtctggctcg gcgggttcgc
     4621 atgccgccgc ctgctcacgc tgaaccggtt cgtgcactgg ctgcacgacg tcttcgaccg
     4681 gccctactgg tcgctcgcgc aacacctgaa ggcgcgcttc ccgggcgcga agggctacgt
     4741 cgagcgctac cagaacgcga cgctcgccgc ggcgcgcgag gcgcgcgtcg acggcgtcgt
     4801 ctgcggccac atccaccgcg ccgcgttcac ggaggtcgac ggcctgctct
//
LOCUS       DASSNN010000645         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_613361, whole genome shotgun sequence.
ACCESSION   DASSNN010000645 DASSNN010000000
VERSION     DASSNN010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613361"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /gene="fusA"
                     /locus_tag="VFL84_12035"
     CDS             complement(<1..1438)
                     /gene="fusA"
                     /locus_tag="VFL84_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET7609400.1"
                     /translation="MARKTPLARYRNIGIMAHVDAGKTTTTERILFYTGVSQRLGEVD
                     DGSAVMDWMEQEQERGITITSAATTCFWKGMSRKLPEHRINIIDTPGHIDFTIEVQRS
                     LRVLDGAVAVFCGVSGVEPQTETVWRQADKYGVPRVAFVNKMDRAGADFLRVLDQIRE
                     RLKAKPVALQLPIGAADEFEGVVDLVTMKAIRWDNKTLGMRVIESDIPAALEPQAREY
                     RAKLLEAAADADERLLEKFLERGDLSPDEIREGLRARCLAGEIVPALCGSAFRNKGVQ
                     ALLDAVVLYLPAPSDRAAVKGVVPGGAEEARSPSDKAPFSAFAFKIASDAAGSLTFFR
                     VYSGVLRSGDSVYVPRRDKTEIVGRLVQMHANERSEIDEVRAGDIAAAVDLKDVTTGD
                     TLADPKHPLTLEMMEFPEPVIAAAIEPKTSADHARLGDALAKLVREDPTLRVHVDAES
                     GQTILSGMGELHLEIVVDRMLREFGVAAN"
     gene            complement(1460..1939)
                     /gene="rpsG"
                     /locus_tag="VFL84_12040"
     CDS             complement(1460..1939)
                     /gene="rpsG"
                     /locus_tag="VFL84_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET7609401.1"
                     /translation="MSRRAQAPRRDSLPDPKFGNAMLAKFINMVMKSGKKSVAEKIVY
                     GAIERMSERVGKDQSRAIELLGQALDNIKPAVEVKSRRVGGATYQVPVEVRAARRETL
                     AMRWAIEAARSRSEKSMAVRLAHELMDASENRGAAVKKREDTHRMADANKAFAHYRW"
     gene            complement(1942..2316)
                     /gene="rpsL"
                     /locus_tag="VFL84_12045"
     CDS             complement(1942..2316)
                     /gene="rpsL"
                     /locus_tag="VFL84_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET7609402.1"
                     /translation="MSTTNQLVRKARTAVKHKSNVPALNSSPQKRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGTL
                     DCGGVGDRRQGRSKYGAKRPKG"
BASE COUNT          396 a          863 c          829 g          494 t
ORIGIN      
        1 cgttggcggc gacgccgaac tcgcgcagca tgcggtcgac gacgatctcg aggtgcagct
       61 cgcccatgcc actcaaaatc gtttgacccg actccgcgtc gacgtggacg cgcagcgtcg
      121 gatcctcgcg cacgagcttc gcgagcgcgt cgccgagccg cgcgtggtct gccgaggttt
      181 tcggctcgat ggccgcggca atcacgggct cggggaattc catcatctcg agcgtgagcg
      241 ggtgcttggg atccgcgagc gtgtcgcccg tcgtgacgtc cttgagatcg acggcggccg
      301 cgatgtcgcc ggcgcgcacc tcgtcgatct ccgaccgctc gttcgcgtgc atctgcacga
      361 ggcgcccgac gatctcggtc ttgtcgcggc gcggcacgta gaccgaatcg ccggagcgca
      421 atacgccgga gtagacgcgg aagaacgtca ggctgccggc cgcgtcgctc gcgatcttga
      481 acgcgaatgc cgagaacggg gccttgtcgc tcgggctccg cgcttcctcg gcgccgcccg
      541 gcacgacgcc cttgacggcg gcgcgatccg acggcgcggg caagtagagc accacggcgt
      601 cgagcagggc ttgcacgccc ttgttgcgga acgccgagcc gcagagcgcc ggaacgatct
      661 cgccggccaa gcagcgcgcg cgcaatcctt cgcggatctc gtccgggctc aagtcgccgc
      721 gctccaagaa tttttcgagc aagcgctcgt ctgcgtccgc cgccgcctcg agcagcttgg
      781 cgcgatactc tcttgcttgc ggctcgagag ccgccgggat gtcgctctcg atgacgcgca
      841 tcccgagcgt tttgttgtcc cagcgaatcg ccttcatcgt gacgagatcg acgacgcctt
      901 cgaactcgtc ggccgcgccg atcggcagct gcagcgcgac gggcttggcc tttaaacgct
      961 cgcggatctg gtcgagcacg cgcaggaaat ccgcgccggc gcgatccatc ttgttgacga
     1021 acgcgacgcg cggcacgccg tacttgtcgg cctggcgcca gacagtctcg gtctgcggct
     1081 cgacgccgct gactccgcag aacaccgcaa cggcgccgtc gagcacgcgc agcgagcgtt
     1141 gcacttcgat cgtgaagtcg atgtggcccg gagtgtcgat gatgttgatg cggtgctcgg
     1201 ggagcttgcg gctcatccct ttccagaagc aggtcgtggc ggccgacgtg atcgtgatgc
     1261 cgcgctcttg ctcctgctcc atccagtcca tgacggccga gccgtcatcg acctcaccga
     1321 gccgctgcga gacccccgtg tagaacagga tccgctcggt agtcgtcgtc ttgccggcat
     1381 cgacgtgagc catgatgccg atattccgat accgcgcgag tggggtcttc cgtgccatgt
     1441 tcgggccgcc cgaccatcac taccagcgat agtgcgcgaa cgctttgttc gcgtccgcca
     1501 tacggtgcgt gtcttcgcgc ttcttgacgg ccgcgccgcg gttctcggaa gcgtccatga
     1561 gctcgtgcgc caggcgcacg gccatgctct tctcggagcg cgaacgcgcg gcctcgatgg
     1621 cccagcgcat cgcgagcgtc tcgcggcgcg ccgcgcgtac ttcgacgggc acctgatacg
     1681 tcgcgccgcc tacgcggcgg gatttcacct cgacggccgg cttgatgttg tcgagcgctt
     1741 gaccgagcag ctcgatggcg cggctttggt ccttgccgac gcgctccgac atgcgctcga
     1801 tggcgccgta gacgatcttc tccgcgacgg acttcttgcc gctcttcatc accatgttga
     1861 tgaatttcgc gagcatcgcg ttgccgaact tcgggtccgg caaggaatcg cgccgcgggg
     1921 cttgtgctct tctcgacatg tttagccttt gggacgctta gcgccgtatt tcgaacgacc
     1981 ttggcgccga tcgccgacgc cgccgcaatc gagcgtgccg cgcacgatgt ggtagcgcac
     2041 gccgggcagg tccttgacgc ggccgccgcg aatcagcacg accgagtgtt cctgcaggtt
     2101 gtggccttcg ccgccgatgt agctcgtaac ctcgaagccg ttcgtcaagc gaacgcgcgc
     2161 gaccttccgc atcgcggagt tcggcttctt cggcgtcgtc gtatagacgc gagtacagac
     2221 gccgcgcttt tgcggcgagg aattgagcgc gggcacgttg ctcttgtgct tgacggcagt
     2281 gcgcgcttta cgaacgagtt ggttggtcgt cgacattcaa tgcttccaaa ataaattttg
     2341 gcccgttacc gagcgttctc ggcagccggg ccttggacgt cctaagccta aacctaagcc
     2401 tcgcgaagga ttttcgggtc gcgaaaccgg tgtcgatgcg ccaggacagc ccgaaattct
     2461 aggcttccgg ccggactgac gtcaaccgca agaggtgccg gtgttacggt ttcggttcat
     2521 ggacctggct tcagcgctcg cgcgccgcct gcctcgaacc gaaccgtaac accggcaccc
     2581 tc
//
LOCUS       DASSNN010000646         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1240446, whole genome shotgun sequence.
ACCESSION   DASSNN010000646 DASSNN010000000
VERSION     DASSNN010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240446"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..515
                     /locus_tag="VFL84_12050"
     CDS             135..515
                     /locus_tag="VFL84_12050"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HET7609403.1"
                     /translation="MSSSTPKTRSKPHGEPAPIPGTVIFDGIEAQKGYALNKMCYSFN
                     STENRAAFARDEDGYCRAYGITPAQRDAIRRRDVLALVNLGGNVYYLAKLAGIFGLGV
                     QDIGAQQTGVTVEEFRARLSAAGG"
     gene            517..1359
                     /locus_tag="VFL84_12055"
     CDS             517..1359
                     /locus_tag="VFL84_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase family protein"
                     /protein_id="HET7609404.1"
                     /translation="MAKLLGAICTSHIPAIGNAIARGKENEPYWRPFFAGYPPIREWL
                     KKVDPAAVVMIYNDHGLSFFLDKMPTFAIGAAPQYRNEDEGWGLPTLPPYTGDPRLSW
                     HMIESLVADEFDVTTCQEMLVDHAFTVPISLLWPERSARRFGTVPVAINTVQHPLPTP
                     ARCYAFGQAIGRAIESYPQDIGVVVVATGGMSHQLDGERAGFINKAFDLYCLEKIVAD
                     PRALTKMSIHELVREAGTQGVELIMWLAARGALDGNVAQLHRHYHIPVSNTAAGLLLL
                     ERAA"
     gene            1439..2047
                     /locus_tag="VFL84_12060"
     CDS             1439..2047
                     /locus_tag="VFL84_12060"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HET7609405.1"
                     /translation="MAQLSILELGNVLFRPSATFARIAERPPSSTAVFFGFALWLGLA
                     PPVFAYIGTTTFGWRLGVEPLFLSAPSAAAIAAAYFALLLVGFLSASFVAHWMASTYS
                     ADASFGRSFALIALVGAPLTVGSVVHLYPHALINVVVLVPALIWSMYLLYRGLPIVLK
                     TDPAHGMLMASSLVAYLLVAWVTLLGITVVLWSHGIGPRVLT"
     gene            2044..>2569
                     /locus_tag="VFL84_12065"
     CDS             2044..>2569
                     /locus_tag="VFL84_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7609406.1"
                     /translation="MSTRSAVDGAAIAPTYHDRIVTAFTIASVFWAIVGMAAGVYVAA
                     ELVWPDLSFSLPWLSFGRLRTVHTNVVLFGFGVSALIGTSLYSVQRTSHTPLYAPRLA
                     WFVFYAWQAALVLGGWSLLAGWNGGKEYAELEWPFDVAITVVWLCYAVVFFGTIAQRK
                     IAPIYISNWFYGALI"
BASE COUNT          391 a          858 c          844 g          476 t
ORIGIN      
        1 gcgccttggt cgacgaggaa ctcgccgtag aaaaaattgt tgtcgatgtt gtcgggatcg
       61 accgcgacgc cgacgagatc gcggccatcg acgccgcgct cgagcggctc gatgcgggca
      121 cttacgggca gtgcgtgtcg agctcaacgc ccaagacgcg ctcgaaaccg cacggcgagc
      181 cggcgccgat acccggcacc gtcattttcg acggcatcga agcccaaaag ggctacgcgt
      241 tgaacaaaat gtgctactcg ttcaacagca ccgagaatcg cgccgcgttc gcgcgcgacg
      301 aggacggcta ctgccgcgct tacggcatca cgccggcgca gcgggacgcg atccggcgcc
      361 gcgacgtgct ggctctcgtc aatcttggcg gcaacgtcta ttacctcgcg aagctcgcgg
      421 gcatcttcgg gctcggcgtg caagacatcg gcgcgcagca aaccggcgtg accgtcgaag
      481 agttccgcgc gcgtctcagc gcggcaggcg gatgacatgg cgaagctgct cggcgcgatc
      541 tgcacgtcgc atatcccggc aatcggcaac gcgatcgcgc gcggcaaaga gaacgagccg
      601 tactggaggc cgttcttcgc gggctaccct ccgataaggg agtggctcaa gaaagtcgat
      661 ccggccgccg tggtgatgat ctacaacgat cacggtctga gcttcttcct cgacaagatg
      721 ccgacgttcg cgatcggcgc ggcgcctcag taccgcaacg aagacgaggg ttgggggctg
      781 ccgacgttgc cgccctacac gggcgatccg cggctctcgt ggcacatgat cgagtcgctc
      841 gtcgcggacg agttcgatgt cacgacctgc caggagatgc tcgtcgatca cgcgttcacg
      901 gtgccgatct cgctgctgtg gccggaacgc tcggcgcgcc gcttcggcac ggtgcccgtc
      961 gcgatcaata ccgtccagca tccgctgccg acgccggcgc gctgctacgc gttcggtcag
     1021 gcgatcggcc gcgcgatcga gtcgtacccg caggacatcg gcgtcgtcgt cgtcgcgacg
     1081 ggcggcatgt cgcatcagct cgacggcgag cgcgcgggct tcatcaacaa ggcgttcgat
     1141 ctgtactgcc tcgagaagat cgtcgccgat ccgcgtgcgc tgacgaagat gtcgattcac
     1201 gagctcgtgc gcgaagccgg aacgcaaggc gtcgagctca tcatgtggct cgccgcgcgc
     1261 ggcgcgctcg acggtaacgt cgcgcagctt catcggcact accacatccc ggtctcgaac
     1321 acggcggcgg gtctgctgct gctcgagcgc gccgcctgac tctcggaaag tacgaatact
     1381 cgcggcacgt gcgagctctt agagtgagcg ctgcaccgcc cccacggacg gcgattcagt
     1441 ggcacaactc tcgatactcg agctcggcaa cgtcctcttc agaccgagcg caacctttgc
     1501 gcggattgcg gaacggccgc cgtcatcgac cgccgtgttc ttcggcttcg cgctctggtt
     1561 gggcctcgcg ccgcccgtct tcgcctacat cggcacgacg acgttcggct ggcggctcgg
     1621 cgtcgagccg ctgtttctca gcgcgccgtc cgccgcagcg atcgcggcag cgtatttcgc
     1681 gttgctgctc gtcgggttcc tgagcgccag tttcgttgcc cactggatgg cgtcgacgta
     1741 ctcggccgac gcgtcgttcg gccgcagctt cgcgttgatc gcgctcgtcg gcgcgccgct
     1801 gaccgtcggc agcgtcgtgc acctgtatcc gcacgcgttg atcaacgtcg tcgtgctcgt
     1861 gccggcgctg atctggagca tgtacctgct gtaccgcggg ttgccgatcg tgctgaagac
     1921 cgatcccgcg cacggcatgc tgatggcgtc gtcgctcgtc gcgtacttgc tcgtggcgtg
     1981 ggtcacgctg ctcgggatca cggtcgtgct ctggagccac ggcatcggcc cgagggtgct
     2041 cacgtgagca cgcgcagcgc agtcgacggc gccgcaatcg cgccgaccta tcacgaccgc
     2101 atcgtcacgg cgttcacgat cgcatcggtg ttctgggcca tcgtcggcat ggcggccgga
     2161 gtctatgtcg cggccgagct cgtctggccg gacttgagct tctcgctgcc gtggttgtcg
     2221 ttcggccggc tgcgcaccgt gcacacgaac gtcgtgctgt tcggcttcgg cgtctcggcg
     2281 ctgatcggca cgtcgctcta tagcgtgcag cggacgtcgc acacgccgct ctacgcgccg
     2341 cggctcgcct ggttcgtctt ctacgcctgg caggccgcgc tcgtgctcgg cggctggtcg
     2401 ttgctcgccg gttggaacgg cggcaaggaa tacgcggagc tcgagtggcc gttcgacgtc
     2461 gcgatcaccg tcgtctggct ctgctacgcg gtggtgttct tcggcacgat cgcgcagcgc
     2521 aagatcgcgc cgatctacat ctcgaactgg ttctacggcg cgctgatca
//
LOCUS       DASSNN010000647         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_486534, whole genome shotgun sequence.
ACCESSION   DASSNN010000647 DASSNN010000000
VERSION     DASSNN010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486534"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VFL84_12070"
     CDS             complement(<1..1185)
                     /locus_tag="VFL84_12070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7609407.1"
                     /translation="MRRVFSPVARITLAMLGITVALVLAAHTLRILPDTEAEALANRK
                     RTVEALTVQLTSGTLLDDLDSTIVVLDAVVERSPEILSAALRDAGGTIVLESGNHEQF
                     WRPTSDGRSTPEFVRAPIFAGDAEIGALEVRFEPLPSQWALSFDRGGVAAFLLFVTIG
                     GALGYFLVLRRSLRALDPSAVVPERVKVAMDTLVEGVIIMDEREQVLLANQAFGRYLD
                     VPVEQLIGKRASALNWRSADTGGSAPALPWVVAMTTRVPVTDCMLELRAANQAIRTFA
                     VNATPVLDARGGLRGALASFGDVTELRQQNGELQRTLTQLEDSRQVVEKHNAELRYLA
                     SHDALTGCLNRRAFFDAFERAFEGAVERKDVLCCAMIDIDHFKSVNDRFGHATGDRVI
                     GFV"
     gene            complement(1191..>2806)
                     /locus_tag="VFL84_12075"
     CDS             complement(1191..>2806)
                     /locus_tag="VFL84_12075"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HET7609408.1"
                     /translation="DAPTTAPVTLAPLPEDGGPRLITNAELVANAADIDGNSLTAAGL
                     AIASGNGTLVDNGNGTWTYTPAADDSGVVAFLYTIADGNGGTVAGSATLDITPVNDAP
                     VVTSNGGGLNASAGVAENTTAVTVVTSVDADGDAVQYSIIGGADAARFRIDGATGALT
                     FLSAPNYEAPADGGADNIYEVIVRAVDGNGGSATQTLQIAVAGVDEAPTAGGDAFEMS
                     DNQLLIVTTTRLLSNDADPEGGALRIVSMTSPLSGTLEQDAAGGWRYTPSSGFTGNDR
                     FTYTVADASGRTATAEVVIAVASLPSGAGGAAPAAPAATSSAAPSAPSGPVSGGAIAS
                     TFGGNPLDGVDVLAGGHVDVAGAEASAVWTVDAEEPPAGDVADSAVADFAAEIERERV
                     YDVRNWAPPDQVELEQIAIDMNNNLLRLALNMGLQDTSRSLAVFDETFQEMADGVRRA
                     DREVAEIAIGSGVAVGAGVIAWLLRGGALVASLLSALPAWASFDPVPILARRHDDERK
                     PPAPPEDPSETAVSRVLRPDALPTRQARS"
BASE COUNT          464 a          985 c          930 g          427 t
ORIGIN      
        1 gacaaacccg atgacgcgat ctccggttgc gtggccgaat cgatcgttga cgctcttgaa
       61 gtgatcgatg tcgatcatcg cgcagcacaa gacatccttg cgctcgaccg cgccctcgaa
      121 cgcgcgttcg aatgcgtcga aaaacgcgcg gcgattcagg caacccgtca gcgcgtcgtg
      181 cgaagcgaga tagcgcaact cggcattgtg cttctcgacg acctggcgag aatcctcgag
      241 ctgcgtaagc gtgcgctgga gctcgccgtt ctgctggcgc aactccgtga cgtcgccgaa
      301 gctcgccaat gcgccgcgca gaccgccgcg cgcatcgagc accggcgtcg cattcacggc
      361 gaacgtgcgg atcgcctggt tcgccgcccg aagctcgagc atgcaatcgg tgacgggcac
      421 gcgtgttgtc atcgccacga cccagggcag cgccggagcg ctgccgcccg tgtccgcgga
      481 gcgccaattc agcgccgacg cccgtttgcc gatgagctgc tcgaccggca cgtcgagata
      541 acggccgaaa gcctgattcg cgagcagcac ctgctcgcgc tcgtccatga tgatcacgcc
      601 ctcgacgaga gtgtccatcg ccaccttgac gcgctcgggc accacggcgc tgggatccaa
      661 cgcgcgcaag gagcggcgca agaccaggaa atatccgagc gctccaccga tggtgacgaa
      721 cagaaggaac gccgcgacgc cgccgcgatc gaacgacagc gcccactgcg acggcaaggg
      781 ctcgaagcgg acctccaacg cgccgatctc ggcgtcgccg gcaaagattg gagcgcgcac
      841 gaactcgggc gtcgagcgcc cgtcggaagt cggccgccaa aattgttcgt ggttgccgga
      901 ctcgagcacg atagtaccgc ctgcatcgcg caacgccgcg gacagaatct cgggactgcg
      961 ctcgacgact gcatcgagca ccacgatcgt gctgtcgaga tcgtcgagca gtgtccccga
     1021 cgtcaactgc accgtcagcg cttcgacggt gcgcttccga tttgcgagcg cctccgcctc
     1081 cgtgtcggga agaatccgca gcgtgtgcgc ggccaagacc agcgcgaccg tgatcccgag
     1141 catggccaag gtgatgcgag cgaccggaga gaagacacgg cgcatgggca tcagctccga
     1201 gcctgacggg tggggagagc atcgggacgc agcacgcggc tgaccgccgt ctcgctcgga
     1261 tcttccggcg gagccggcgg cttccgctcg tcgtcgtgac gccgcgcgag aatcggaacc
     1321 gggtcgaagg aagcccaggc cggcaacgcc gacagcaacg acgccacgag cgcgccgccg
     1381 cgcaatagcc acgcaatgac gccggcgccg accgcgacgc cggatccgat cgcaatctcg
     1441 gcaacttcac gatccgcgcg gcgcacgccg tcggccatct cctggaacgt ctcgtcgaag
     1501 accgccagcg agcgcgaggt gtcctgcaat cccatgttca gcgcaagtct gagcaggttg
     1561 ttgttcatgt cgatcgcgat ctgctcgagc tcgacttgat cggggggcgc ccagttccgc
     1621 acgtcgtaga cgcgttcccg ctcgatctcc gcggcgaagt cggcgacggc actgtctgcc
     1681 acgtcgccgg ccggcggctc ctccgcgtct acggtccaaa cagcagacgc ctctgcgccc
     1741 gccacgtcga catggccacc cgcgaggacg tcgaccccgt cgagcgggtt gccgccgaac
     1801 gtgctcgcaa tggcgccgcc gctcaccggc ccggacggcg ccgacggcgc cgcggaagac
     1861 gtcgccgcag gcgccgcagg cgcagcgccg cccgcccctg acggcagaga cgcgacggcg
     1921 atgacgacct ccgcggtggc cgttcgtccg ctcgcgtccg ccaccgtata cgtgaaccgg
     1981 tcgttgcccg tgaacccgct cgacggcgta tagcgccaac cgcccgcggc atcctgctcg
     2041 agcgtcccgg acaacggact ggtcatcgac acgattcgca gcgcaccacc ctcgggatcc
     2101 gcgtcgttgg agagcaagcg tgtcgtcgtc acgatcaaca attggttgtc ggacatttcg
     2161 aatgcatcgc cgccggcggt cggcgcctcg tcgacgcccg cgacggcgat ttggagtgtc
     2221 tgcgtggccg agccgccgtt gccgtccacg gcgcgcacga tgacttcgta gatgttgtct
     2281 gcgccgccgt cggcgggcgc ctcgtagttc ggagccgaga ggaatgtcag cgcgcccgtc
     2341 gcgccgtcga tgcggaaccg cgcggcatcg gcgccgccga tgatcgagta ctggacggcg
     2401 tcgccatccg catcgacgct cgtcacgacg gtgaccgccg tcgtgttctc ggcgacgccc
     2461 gcgctcgcat tgaggccgcc gccattcgag gtcacgaccg gcgcgtcgtt caccggtgtg
     2521 atgtcgagcg tggcgctgcc cgcgaccgtg ccgccgttgc cgtcggcgat cgtgtagagg
     2581 aaggcgacta cacccgagtc gtcggcggcc ggcgtgtacg tccacgtgcc gttgccgttg
     2641 tcgacgagcg tgccgttgcc gctcgcgatc gcgaggccgg cggccgtcag ggaattgccg
     2701 tcgatgtcgg ccgcgttcgc gacgagctct gcgttcgtga tcaggcgcgg cccgccgtcc
     2761 tccggcagcg gcgccaatgt gactggcgcc gtcgtcggcg cgtcgt
//
LOCUS       DASSNN010000648         4088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_109314, whole genome shotgun sequence.
ACCESSION   DASSNN010000648 DASSNN010000000
VERSION     DASSNN010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109314"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1714)
                     /locus_tag="VFL84_12080"
     CDS             complement(<1..1714)
                     /locus_tag="VFL84_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7609409.1"
                     /translation="MAKDRLKRRIAEALQRSSARDELKLILAGDGLERAAARGVLHPI
                     LENVKDPIRAVAGDGIVQEANTAAARLLDAAVGDIVGRDIARFIPTLVPALPTLEALA
                     ERVADTFVDATPELIAVRRNDGRPLTVEVTVSRAGFGPSTCFVLCMRDVTERLHDEQA
                     LRDSEARYRALVENAPEAIVVLDVDRNVFVEVNDNAVKLFKLSREELLLLGPEALCPE
                     RQSDGQPSHDLHLGYVERALRGARPVFEWLHRDGQGKEIPCEVRFIHLPSSKQRLIRA
                     SIIDNAARRQADTLAYGERRVLELVAANAPLEKTLQAVVRLVEQLHPGVGASISLLDA
                     HAGELALAAANALETAMSEALAKLPIGLRSGSCGVAASLGRQVVVRDVATDPLWAELA
                     AVAAANGVRACCSTPIVTADDRVLGTLALYFATVRGPNTEELDLVTRLTQLAGIAIRR
                     KQDETALRESEARFRSLFDNVVDGVYQAAPSGELKSANPALVHMLGLDEGALGRCNMA
                     EFFVDAKERARLVSELMSYGRVRNFEYQLRTRAGRVIVVIENSRLVSSADGSPLYFEG
                     TITDI"
     gene            complement(1813..2316)
                     /locus_tag="VFL84_12085"
     CDS             complement(1813..2316)
                     /locus_tag="VFL84_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609410.1"
                     /translation="MRARALLLLAVLACPALGTAAPPADERPAPARLRVPGKPTGPIA
                     VEYTLAGAPAVGVPLEIAVKARVEADVKGLAIEANPSAPRAVLVTPPELVTAADGVYS
                     WTITVVPLAVEAGYLSVIVAGRVDGVAQARGVTVSLHGAAVQGAAPSAGPADPEALIA
                     LPVEETP"
     gene            complement(2313..4037)
                     /locus_tag="VFL84_12090"
     CDS             complement(2313..4037)
                     /locus_tag="VFL84_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609411.1"
                     /translation="MGRGYDRAMLRLLVSVSLVALLAGCGSSGSADPGVPADSVSLSG
                     QVTFDRVPAVAGQGLVYAQTTVQPARGVTVELLEAGAVRASTTTDATGHYVFAGAPQN
                     TDVSVRVRAEMLRIGTPSWNFRVVDNVNGEALYTLAGAVFNTGTSDVTRNLHAASGWD
                     GSAYTATRSAAPFAILDVAYDAVQLVLTAEPSTVFPALRFHWSPANVPVLGTAPGEIG
                     SSRFQPDVGILLVGAVDQDTDEYDRHVIAHELGHYLEYHFSRSDSIGGPHALSDQLDM
                     RLAFGEAWGTAFAAMATGDAVYTDTFGAGQRHFFAVDVEQRPSRTNPNPGWFNEESLQ
                     SLLYDLYDNGRDVPPGSLVVDDLSLGFGPIWHAFANEHRTTRALTSVFPFVDALKVAR
                     PADAALIDNLTRSQRIADVGDAFGTGQTNFGVPTKRSLAEVSLDFNTVYGTLAVGATL
                     QNVCSLDDYTSSFTGAENKVASRRFVRFTVTNPGQHTITVRARAPLNAPADPDAVLHL
                     GGGRKIVSETPPSCSVQTPSACVEEFSPTLSAGEHVLEVYEWTNTNALDDADPPIGRT
                     CFDVTVTR"
BASE COUNT          637 a         1423 c         1402 g          626 t
ORIGIN      
        1 tgatgtcggt gatcgtgccc tcgaagtaca gcgggctgcc gtccgccgag ctcacgagcc
       61 gcgaattctc gatgacgacg atgacgcggc ccgcgcgcgt cctgagctga tactcgaagt
      121 tgcggacgcg gccataggac atcagctcgc tgacgaggcg ggcgcgctcc ttcgcgtcca
      181 cgaagaactc ggccatgttg cagcggccca gcgcgccctc gtcgagaccg agcatgtgca
      241 cgagcgccgg gttcgccgac ttgagctcgc cgctcggcgc cgcctggtac acgccgtcca
      301 cgacgttgtc gaacagcgag cggaacctcg cctcgctctc ccgcagcgcg gtttcgtcct
      361 gcttgcggcg aatcgcgatg ccggcgagct gcgtcaggcg cgtgacgagg tcgagctcct
      421 ccgtgttcgg gccgcgcacc gtggcgaaat acagcgcgag cgtgccgagc acccggtcgt
      481 cggccgtgac gatcggcgtc gagcagcagg cccgcacgcc gttggccgcg gcgacggccg
      541 cgagctcggc ccagagcgga tccgtcgcga cgtcgcgaac gacgacctgc cggccgagcg
      601 atgccgcgac gccgcacgag cccgagcgca agccgatcgg caatttcgcc aacgcttcgg
      661 acatggccgt ctccaaagcg ttggccgcgg cgagcgcgag ctcgccggca tgcgcgtcga
      721 gcaagctgat cgaagcgccg acgccgggat gcagctgctc gacgagccgc acgacggcct
      781 gcaaggtttt ttcgagcggc gcattcgccg cgacgagctc gagcacgcgg cgttcgccgt
      841 aggccagcgt gtccgcctgg cggcgcgcgg cgttgtcgat gatgctcgcg cggatcaggc
      901 gctgcttcga cgacggcaaa tggatgaagc gcacctcgca ggggatctcc ttgccttgtc
      961 cgtcgcggtg cagccattcg aagacgggcc gcgcgccgcg caaggcgcgc tcgacgtagc
     1021 ccaagtgcaa gtcgtgcgac ggctgaccgt cgctctggcg ctcggggcac agggcttcgg
     1081 gacccaaaag cagcagctct tcgcgggaga gcttgaacag cttgacggcg ttgtcgttga
     1141 cctcgacgaa cacgttgcgg tcgacgtcga gcacgacgat cgcctcgggc gcgttctcga
     1201 cgagcgcgcg atagcgcgct tcgctgtcgc gcagcgcttg ctcgtcgtgg agccgctccg
     1261 tgacgtcgcg catgcagagc acgaagcacg tgctcggtcc aaaaccggcg cggcttacgg
     1321 tgacctcgac cgtcaacggc ctaccgtcgt tgcgccgcac ggcgatgagc tccggcgtcg
     1381 cgtcgacgaa cgtgtccgca acgcgctcgg cgagagcctc gagcgtcggc agcgcgggca
     1441 cgagcgtcgg aatgaaacga gcgatgtcgc ggccgacgat gtcgccgacc gccgcgtcga
     1501 gcagccgcgc ggcggccgta ttcgcttcct gcacgatgcc gtcgccggcg accgcgcgaa
     1561 tcgggtcctt gacgttctcg aggatcggat gcagcacgcc gcgcgcagcg gcgcgttcca
     1621 atccgtcgcc ggcgaggatc agcttcagct cgtcgcgtgc cgacgagcgc tgcaacgctt
     1681 cggcaatcct gcgctttagg cggtccttcg ccatcgacaa taccaaggcc tccggccccg
     1741 ccggtgaatg ggcaaaattc taggaacggc ttggccttag ccgccgtgac tcaactctcg
     1801 tttaccgccg ttttagggag tttcctcgac ggggagcgcg atcagagcct cggggtccgc
     1861 ggggccggcg ctcggcgcag ccccttgcac ggcggcgccg tgcagcgaca ccgtgacgcc
     1921 gcgcgcttgc gcgacgccgt cgacgcggcc cgcgacgatc acgctcaagt agcccgcctc
     1981 gacggccagc ggcacgaccg tgatcgtcca ggaatagacg ccgtcggcgg ccgtgacgag
     2041 ctccggcggc gtgacgagga ccgcgcgagg cgcgctcggg ttcgcttcga tcgcgaggcc
     2101 cttcacgtcg gcctcgactc tcgctttgac cgcgatctcg agcggcacgc cgacggccgg
     2161 cgcccccgcg agcgtgtact cgacggcgat cggccccgtc ggcttgccgg gcacgcgaag
     2221 ccgcgccggc gccgggcgct cgtccgcggg cggcgccgcc gtgccgagcg cggggcacgc
     2281 gagaaccgcc aacagcaaca gtgctctcgc cctcatcgcg tcaccgtcac gtcgaagcac
     2341 gttcggccga tcggcggatc cgcatcgtcg agcgcgttcg tgttcgtcca ttcgtaaacc
     2401 tcgagcacgt gctctcctgc cgacagcgtc ggcgagaatt cctcgacgca ggccgaaggc
     2461 gtttgcacgg agcagctcgg aggcgtttcc gacacgatct tgcggccgcc gcccaagtgc
     2521 agcacggcgt cgggatccgc cggcgcgttg agcggcgcgc gcgcgcgcac cgtgatcgtg
     2581 tgctgccccg gattcgtgac cgtgaagcgc acgaagcggc gcgacgcgac cttgttctcg
     2641 gctcccgtga acgagctcgt gtagtcgtcg aggctgcaca cgttctgcaa cgtcgcgccg
     2701 acggccaacg tgccatagac cgtgttgaaa tccagcgaga cttcggcgag cgaccgcttg
     2761 gtcggcacgc cgaagttcgt ttgtcccgtg ccgaacgcgt cgccgacgtc cgcgatgcgc
     2821 tgcgacctcg tcaggttgtc gatcagcgcc gcgtcggccg gacgcgcgac tttgagcgcg
     2881 tcgacgaacg ggaacacgct cgtcaacgcg cgcgtcgtgc ggtgctcgtt cgcgaatgcg
     2941 tgccagatcg ggccgaaccc gagcgacagg tcgtcgacga cgagcgagcc gggcggcacg
     3001 tcgcggccgt tgtcgtagag gtcgtagagc agcgactgca gcgactcctc gttgaaccat
     3061 cccggattcg gattcgtgcg gctcgggcgt tgctcgacgt cgacggcgaa gaaatgccgt
     3121 tgccccgcgc cgaacgtgtc cgtgtaaacc gcgtcgccgg tcgccatcgc cgcgaacgcg
     3181 gtcccccagg cttcgccgaa cgcgagcctc atgtcgagct ggtcggagag cgcgtgcggg
     3241 ccgccgatgc tgtccgagcg cgagaagtgg tattcgagat agtgaccgag ctcgtgcgcg
     3301 atgacgtgcc ggtcgtattc gtccgtgtcc tgatctaccg cgcccacgag cagaatcccg
     3361 acgtccggtt gaaaccggct cgagccgatc tcgcccggag ccgtgcccaa aaccggcacg
     3421 ttcgcggggc tccagtgaaa gcgcagcgcc gggaaaaccg tcgacggctc ggccgtcagc
     3481 acgagctgca cggcgtcgta cgcgacgtcg aggatcgcga acggcgcggc ggagcgcgtc
     3541 gccgtgtaag ccgagccgtc ccatcccgaa gccgcgtgca gattgcgcgt gacgtcggac
     3601 gtgcccgtgt tgaacacggc gcccgcgagc gtgtacagcg cctcgccgtt cacgttgtcg
     3661 acgacacgga agttccagct cggcgtaccg attcggagca tctccgcgcg cacgcggacg
     3721 gaaacgtccg tgttctgcgg cgcgcccgcg aagacgtaat ggcccgtcgc atccgtcgtc
     3781 gtgctcgcgc ggacggcgcc cgcctcgagc agctcgaccg tgacaccgcg cgcgggctgc
     3841 accgtcgtct gcgcatagac gaggccctga ccggcgacgg cggggactcg atcgaacgtg
     3901 acttggccgc tcagcgagac gctgtcggcc ggcacgccgg gatcggcgct gccgctgctg
     3961 ccgcagccgg cgagcagcgc cacgagcgaa accgaaacga gcagccgcag catcgctcga
     4021 tcgtaaccgc gccccatggc cttatcacag tgtctgtgtc ccgcgacctg cggttttcta
     4081 acccaaaa
//
LOCUS       DASSNN010000649         3579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_613802, whole genome shotgun sequence.
ACCESSION   DASSNN010000649 DASSNN010000000
VERSION     DASSNN010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613802"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VFL84_12095"
     CDS             <1..1263
                     /locus_tag="VFL84_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7609412.1"
                     /translation="ALVNWSATDSIRIRGGFQVATRQANTEELFAGPRLNTVGDFIYG
                     DPCQVSTTAPWGNRPPSQYPATAATHGNRNDPSGPNPNYLQVQQLCAALINRSDTNPA
                     NDDNSAFDTNTGSAQYPRAPGAPNGRDSFTRPGFPFFQAENEIPRGNPNVNPEEAETW
                     TLGVVFAGPGSLENLTASVDFYDIEITDVIATIDSTFVYSKCLNADGVSNPTYSLDDP
                     GGYCDMIGRQVQTGERDTVQAPYVNSGMLFTRGIDFGINWTRDLGDGGSSFYVNSQLT
                     YLDEFKIQDAPGEIILDVRDTLSTTYYGAQYKYKMYNTFGYNLPNGRASLALNWRYLP
                     EIRSETAARNPATTQLGADSYSVFGLNTRVMINDRIEFRGGIDNLFDEDPVVVEVRPG
                     VDSNTDVTRTEYYDALGRRAYVGLKVSF"
     gene            complement(1326..1892)
                     /locus_tag="VFL84_12100"
     CDS             complement(1326..1892)
                     /locus_tag="VFL84_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609413.1"
                     /translation="MKRAQMDKANGFARSARACAAVLAAALAAPVAAQQSADASAADD
                     AADGPQACLNQNEIRRATILNDRNIVFTTRHDVIYSNQLPKQCPGLNRKSIVNYPITN
                     RRLCAGDRFQVLWEQAPGRFAPAAMCPLGTFVPITATELEDLASSTDDNRGRRQRGRS
                     AREAVTTEQVELPPAATPPEAAAPTAPE"
     gene            2005..>3579
                     /locus_tag="VFL84_12105"
     CDS             2005..>3579
                     /locus_tag="VFL84_12105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7609414.1"
                     /translation="MSVPPGVSERDFQRAIARFTSVVGADWVFTSDEDTALYRDAYSP
                     LVGEPEEKVASAAVAPDSAEQVREIVRIANELRIPLYPISTGRNLGYGGSAPVLSGSV
                     VLDLKRMNRILEVDERNAYALVEPGVSYFDLYRHIEERGLKLWIDCPDPGWGSVVGNA
                     LDHGVGYTMNPYRDHWGAQCGLEVVTGDGELVRTGMGALPGSKTWQQFKYGFGPYLDG
                     MFSQSNFGVVTKMGIWLMPQPEAYVDCTVEVFGHDDIIPLVDHMSYVMNTGLQNAGTN
                     LQSPIRFAAARDPELRAALASDDPADIAKLNAFARERQVPFYSNIFKFYGPEQVVRAQ
                     LDYTRRRLSSIPGARVRDGAFYRFPITAEQKAAVRDPQDLGIPTLETFSIGARSNFGP
                     ATRGHLGFSPVIPMRGEAVIESQRVFQRVLQEFGTDVALLVFPQSYHPRTFVILCMFY
                     VMPDVRSNQAVRRTFSKLVEVAAEHGWGEYRTHTAFMDECAGAYSFNDHALRKLHEKI
                     KDAVDPNGVLSAGRYGI"
BASE COUNT          593 a         1190 c         1191 g          605 t
ORIGIN      
        1 gcgctcgtca actggtcggc gacggactcc attcggatcc gcggcggctt ccaggtcgcg
       61 acgcgccagg ccaacacgga agagctgttc gcgggcccgc ggttgaacac ggtgggcgac
      121 ttcatctacg gcgacccgtg ccaggtctcg acgacggcgc cgtggggcaa tcgtccgccg
      181 agccagtacc ccgcgacggc cgcgacgcat ggcaaccgca acgatccgag cgggccgaac
      241 ccgaactacc tgcaagtgca gcagctctgt gcggccctca tcaaccgcag cgacacgaac
      301 ccggccaacg acgacaactc ggcgttcgac accaatacgg gctcggctca gtatccgcgc
      361 gcccctgggg ctccgaacgg gcgcgactcg ttcactcgac ccggcttccc cttcttccag
      421 gccgagaacg agatcccgcg cggcaacccg aacgtcaacc ccgaggaagc cgagacgtgg
      481 acgctcggcg tcgtgtttgc ggggccgggt agcctcgaga acctgaccgc gtcggtggac
      541 ttctacgaca tcgagatcac ggacgtcatc gcgacgatcg attcgacgtt cgtctacagc
      601 aagtgcttga acgcggacgg cgtcagcaac ccgacgtact cgctggacga cccgggtggc
      661 tactgcgaca tgatcggccg ccaggtgcaa accggcgagc gcgacacggt ccaggcgccg
      721 tacgtgaact cgggcatgtt gttcacgcgc ggcatcgact tcggcatcaa ctggacgcgc
      781 gatctcggcg acggcggcag ctcgttctac gtcaacagcc agctcacgta cttggacgag
      841 ttcaagatcc aggatgctcc cggcgagatc attctcgacg tgcgcgacac gctgagcacg
      901 acgtactacg gagcgcagta caagtacaag atgtacaaca cgttcgggta caacctgccg
      961 aacggcaggg cgagcctggc gctgaactgg cgttacttgc cggagatccg gtccgagacg
     1021 gcggcgcgca atcccgccac cacgcagctc ggcgccgatt catacagcgt gttcggtttg
     1081 aacacgcgcg tcatgatcaa cgaccggatc gagttccgcg gcggcatcga caacctgttc
     1141 gacgaggatc ccgtcgtcgt cgaggtgcgg ccgggcgtcg acagcaacac cgacgtcacg
     1201 cggaccgagt actacgacgc gctcggacgc cgcgcctatg tcggcttgaa ggtcagcttc
     1261 taaccgtccg gggggatggg aaagcgacgg cgggccttcg ggcccgccgt tttttacgct
     1321 gcggtttact cgggagcggt cggggcggca gcctcgggcg gtgttgccgc cggcggcagc
     1381 tcgacttgct ccgtcgtgac ggcctcgcgc gcgcttcggc cgcgctgccg gcggccgcgg
     1441 ttatcgtcgg tgctcgacgc gaggtcctcg agctccgtcg ccgtgatcgg cacgaacgtg
     1501 ccgagcgggc acatggccgc gggcgcgaaa cggcccggcg cctgctccca gagcacctgg
     1561 aagcggtcgc cggcgcagag gcggcggttc gtgatcggat agttcacgat gctcttgcgg
     1621 ttcaggccag ggcactgctt cggcagctgg ttgctgtaga tgacgtcgtg ccgcgtcgtg
     1681 aacacgatgt tgcgatcgtt caggatcgtc gcgcggcgga tctcgttttg attgaggcag
     1741 gcttgcgggc cgtccgccgc gtcgtccgcg gcgctcgcgt cggcgctctg ttgcgcagcg
     1801 acgggcgcgg ccagggccgc ggcgagcacg gcggcgcagg cgcgcgccga tcgcgcgaac
     1861 ccgttggcct tgtccatctg cgctcgcttc aacgtggcct cagcgacgta gtctcggcgc
     1921 cgccccgata agttcattcg acccggcttg ccattttgct ccgctcgggc gagtctcgcc
     1981 gctcgagatt cgaggagctt cggaatgagc gtaccaccgg gcgtcagcga gcgggatttt
     2041 cagcgggcca tcgcgcgctt tacgtccgtg gtcggggccg attgggtctt cacgagcgac
     2101 gaggacacag cgctctaccg cgacgcgtac tcgccgctcg tcggcgagcc cgaagagaag
     2161 gtggcgtcgg cggcggtggc ccccgattcc gcggagcaag tgcgcgaaat cgtccgcatc
     2221 gcgaacgagc tccgtattcc tctttatccg atctccaccg gccgcaactt gggctatggc
     2281 ggctcggcgc cggtgttgtc gggcagcgtc gtgctcgatc tgaagcgcat gaaccgtatt
     2341 ctcgaggtcg acgagcggaa cgcgtacgcg ctcgtcgagc ccggcgtcag ctacttcgat
     2401 ctctatcgcc acatcgagga gcggggactc aagctctgga tcgactgtcc cgatcccggc
     2461 tggggcagcg tcgtcggcaa cgcgctcgat cacggcgtcg gctacacgat gaacccgtac
     2521 cgcgatcact ggggcgcgca gtgcggcctc gaggtcgtga ccggcgacgg cgagctcgtg
     2581 cgcacgggca tgggcgcgct gccgggctcg aaaacctggc agcagttcaa gtacggcttc
     2641 gggccgtacc tcgacggcat gttctcgcag tcgaatttcg gcgtcgtgac gaagatgggt
     2701 atctggctca tgccgcagcc cgaagcttac gtcgactgca cggtcgaggt gttcggccac
     2761 gacgacatca tcccgctcgt cgaccacatg agctacgtca tgaacacggg cctacagaac
     2821 gcgggcacga atctgcaatc gccgattcga ttcgcggcgg cgcgcgatcc ggagctgcgc
     2881 gcggcgctcg cgagcgacga tcccgccgac atcgcgaagc tgaacgcgtt cgcgcgcgag
     2941 cgccaggtgc cgttctactc gaacatcttc aagttctacg ggcccgagca ggtcgtgcgc
     3001 gcgcagctcg actacacgcg ccggcgcctg tcatcgatac cgggcgcgcg cgtgcgcgac
     3061 ggcgcgttct accggtttcc gatcacggcc gagcaaaaag cggcggtgcg cgatccgcag
     3121 gacctaggga tcccgacgct cgagacgttc tcgatcggcg cgcgctcgaa tttcgggccc
     3181 gcgactcgcg gccacctcgg gttctccccc gtgatcccga tgcgcggcga agccgtcatc
     3241 gaatcgcagc gcgtgttcca gcgcgtgctg caggagttcg gtaccgacgt cgcgctgctc
     3301 gtgtttccgc agtcgtatca cccgcgcacg ttcgtgatcc tctgcatgtt ttacgtcatg
     3361 cccgacgtcc gatcgaacca ggccgtgcgc cgcacgttca gcaagctcgt cgaagtggcg
     3421 gccgagcacg gctggggcga gtaccgcacg catacggcgt tcatggacga atgcgcgggc
     3481 gcctactcgt tcaacgatca tgcgctgcgc aagctgcacg agaagatcaa ggacgcggtc
     3541 gatccgaacg gcgtcctctc ggcggggcgc tacggcatt
//
LOCUS       DASSNN010000650         3479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_109653, whole genome shotgun sequence.
ACCESSION   DASSNN010000650 DASSNN010000000
VERSION     DASSNN010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109653"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2390)
                     /locus_tag="VFL84_12110"
     CDS             complement(<1..2390)
                     /locus_tag="VFL84_12110"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET7609415.1"
                     /translation="MAYTLLGHDFTPPDLRAKVTGKAKYAEDARADGMLFCRLLTSTM
                     PHARVKIDATEALAMDGVVAVLTADDVPPANAPAEPILTNEPLFVGDRILAVAARSEE
                     LAQEAIDRIKVEYEPLPYVIDPLASLFPAGPDARSDGNVVTQQGVKQIKWTAADFSDA
                     GDTKLPMGTPGAEWSFGDVEAGFQQAKYVLDESFVTAALSHHCLEPRSCMAYWQGDKV
                     FIYGSTQSQSAVMPALARLAGVEPDNVVYIAEFCGGGFGSKINPYPAMGVPIYMAKKT
                     GMPVMMRVSRHEEALFGSSRPAFQGRLRMGFRADGRVLAADLSIVQQNGPYNGGGDLG
                     AAAGAITLLYQPLAMRYRGIQVSTNTTPTGAQRGPGQNQIAAAIEPLIDKAAKALNLD
                     RVAIRRINAADNNAKYDSQQGPVTSAYQREALERGAAAFGWETRKERSGRANGSKVTG
                     VGIGQAFHSAGRGGLDGIVRITPDGKVHIHCGVGNLGTYSYASTSRVAAEVLKASWDN
                     CVIVRGDSRLGLAFSSAQSGSNTSFTMSRATYAGAMDALAKLKEIAAQTLGGTAGDYD
                     VENETVFAKADRSRSMTYAAAAQKAIELGGKFSGQVVPDDVNAVTKSGVAVIAGTGLI
                     GVAKDNIAVTGMVPAIAVGFVEIELDRETGKIDIVDYLGVADCGTVLHPMGLNAQIRS
                     GAVMGFGMGLSERHIYDPQNGLPGNVGLIQAKPASYLDVPSQMQTLAVDINDPQNPVG
                     SKGIGEPVEGCATAAVLAAIADALGGHYFNRVPVLPDMIVNAAAGREQSHKPLQVST"
     gene            complement(2514..3143)
                     /locus_tag="VFL84_12115"
     CDS             complement(2514..3143)
                     /locus_tag="VFL84_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609416.1"
                     /translation="MTAIADNAARQAIVTYLLEALKGRSAKELRIAFLTRDAVAEVVS
                     AVIRDFVAELAAIFRDRDPAMEPLAGELGPDTFAGIQWRHPTWRRGTAIRLEFQRSNR
                     AAPIAGLCAPSERALVGAVGGQHEALDQPTRDKISAALANARGVTLLGRDSAWWPAYQ
                     YLEPPLDDWNTAHALMRVAGIELHEGRMCRDWLADEFVAIRQAVRPIIG"
     gene            complement(3140..>3479)
                     /locus_tag="VFL84_12120"
     CDS             complement(3140..>3479)
                     /locus_tag="VFL84_12120"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET7609417.1"
                     /translation="PHAGEQVRQLEDYAEHLSREYDDRFWVIYLSGDGRGPTTLGEWD
                     MRLRRDGRSCTIAYGNRGARQGRSTLQEWVKACAHLADADKIRWFLLDFEQWIGRTFD
                     APVSTSEARA"
BASE COUNT          521 a         1192 c         1149 g          617 t
ORIGIN      
        1 gtgctgactt gcagcggctt gtgcgactgc tcgcggcccg cggccgcgtt gacgatcatg
       61 tcgggcagca cggggacccg gttgaagtag tggccgccga gcgcgtcggc gatcgccgcg
      121 agcacggccg ccgtcgcgca gccttcgacg ggctcgccga tgcctttcga gccgaccggg
      181 ttctgcggat cgttgatgtc gaccgcgagc gtctgcatct gcgacggcac gtcgagatac
      241 gacgccggct tcgcctggat caggccgacg ttgccgggca agccgttctg cggatcgtag
      301 atgtggcgct cggagaggcc catgccgaag cccatgacgg cgccgctgcg gatctgcgcg
      361 ttcaggccca tcgggtgcag cacggtgccg caatcggcga cgccgagata gtcgacgatg
      421 tcgatcttgc cggtctccct gtcgagctcg atctcgacga agccgacagc gatcgcgggc
      481 accatgcccg tgacggcgat gttgtccttc gcgacgccga tgagccccgt gcccgcgatc
      541 acggcgacgc cggacttcgt cacggcgttg acgtcgtcgg gcaccacttg gccgctgaac
      601 ttgccgccga gctcgatcgc tttctgcgcg gccgccgcgt acgtcatgct gcgcgagcgg
      661 tcggctttcg cgaacacggt ctcgttctcg acgtcgtagt cgccggccgt gcccccgagc
      721 gtctgcgccg cgatctcctt gagcttcgcg agcgcgtcca tcgcgcccgc gtacgtcgcg
      781 cgcgacatcg tgaacgacgt gttgctgccc gactgcgccg acgagaacgc caagccgagc
      841 cgcgagtcgc cgcgcacgat gacgcagttg tcccagctcg ctttcagcac ctcggccgcg
      901 acgcgcgagg tggacgcgta cgagtacgtg cctaaattgc cgacgccgca gtggatgtgc
      961 accttgccgt ctggcgtgat gcgcacgatg ccgtcgaggc cgccgcggcc ggccgagtga
     1021 aacgcttgac cgatgccgac acccgtcact ttcgagccgt tcgcccggcc gctgcgctct
     1081 ttgcgcgtct cccaaccgaa cgcggccgcg ccgcgctcga gcgcctcgcg ctgatacgcg
     1141 ctcgtgacgg gcccttgctg tgagtcgtac ttggcgttgt tgtcggccgc gttgatgcgg
     1201 cggatcgcga cgcgatcgag attcaatgcc ttcgcggctt tgtcgatcag cggctcgatc
     1261 gcggccgcga tctggttctg gcccggaccg cgctgcgcgc ccgtcggcgt cgtgttcgtg
     1321 ctgacctgga tgccgcgata gcgcatcgcg agcggctgat agagcagcgt gatcgcgccg
     1381 gccgcggcgc cgagatcgcc gccgccgtta tacggaccgt tctgctggac gatcgagaga
     1441 tcggccgcga ggactcgccc atcggcccgg aagcccatcc tgaggcgtcc ttggaacgcg
     1501 ggccgggacg agccgaacag cgcttcctcg tggcggctca cgcgcatcat gaccggcatg
     1561 ccggtcttct tcgccatgta gatcggcacg cccatcgcgg gatacgggtt gatcttcgag
     1621 ccgaagccgc cgccgcagaa ctccgcgatg tagacgacgt tgtcgggctc gacgccggcg
     1681 agccgcgcaa gcgccggcat gacggcgctc tggctctgcg tcgaaccgta gatgaagacc
     1741 ttgtcgccct gccaatacgc catgcagctg cgcggctcga ggcagtgatg cgacagcgcg
     1801 gccgtcacga acgactcgtc gagcacgtac ttcgcttgct ggaatccggc ctcgacgtcg
     1861 ccgaacgacc actcggcgcc gggcgtgccc atcggcagct tggtgtcgcc ggcgtcggag
     1921 aagtcggcgg ccgtccactt gatctgcttg acgccttgct gcgtgacgac gttgccgtcg
     1981 ctgcgcgcgt cgggccccgc gggaaacagg ctcgcgagcg gatcgatgac gtacggcagc
     2041 ggctcgtact cgaccttgat gcggtcgatg gcttcctgcg caagctcctc gctgcgcgcg
     2101 gcgacggcga ggatgcggtc gccgacgaac agcggctcgt tcgtcaggat cggctcggcg
     2161 ggcgcgttgg ccggcggcac gtcgtcggcc gtcagcacgg cgacgacgcc gtccatcgcg
     2221 agcgcctcgg tcgcgtcgat cttgacccgc gcatgcggca tcgtgctcgt cagcaggcgg
     2281 cagaacagca tgccgtcggc gcgagcgtcc tcggcgtact tggccttccc ggtgaccttg
     2341 gcccgcaaat cgggcggcgt gaaatcgtgc cccagaagcg tgtaagccat gagctgtctc
     2401 ccccgccgga cccggcggcg cgtatgcggc cgggcgcctc gtttcggccg attctgcggc
     2461 cgctgctcca tggcttcaag caggggaccg ccgagggcgc ggcggcgaac gagttacccg
     2521 attatcgggc ggacagcctg ccggatggcg acgaactcgt ccgccagcca gtcgcggcac
     2581 atgcggcctt cgtgcaactc gatccctgcg acacgcatga gcgcatgagc cgtgttccaa
     2641 tcgtccagcg gaggctcaag gtactgatag gctggccacc aagcggaatc gcgaccgagc
     2701 aatgtgacgc ctcgcgcgtt tgcgagagcc gctgaaatct tgtctcgcgt aggttgatcc
     2761 agcgcttcgt gctggccgcc taccgcacct actagtgccc gttccgacgg cgcgcagagg
     2821 cccgcaatcg gcgccgcacg gttcgagcgc tgaaactcca agcggatcgc cgtgccccga
     2881 cgccacgtcg gatgccgcca ctggatcccg gcaaacgtgt caggcccgag ctcgcccgcc
     2941 aatggttcca tagcggggtc ccggtctcga aatatcgcgg ccaactcggc gacgaaatcc
     3001 cgaatcaccg cgcttacgac ttcggccacg gcgtccctcg tcaggaacgc gattcgcagt
     3061 tccttcgcgc tcctgccctt tagcgcttcc aataggtagg tgacgatcgc ttgccgggcg
     3121 gcgttatcgg cgatggccgt catgctcgtg cttccgatgt cgaaacaggc gcgtcgaagg
     3181 tcctgccgat ccactgctcg aagtcgagaa gaaaccatcg gatcttgtcg gcgtcggcga
     3241 gatgcgcgca cgctttgacc cactcctgaa gtgtcgaccg gccttgccta gcaccgcgat
     3301 tgccgtatgc gatcgtgcag ctacgaccgt cacggcggag acgcatgtcc cactcgccta
     3361 acgtcgtcgg ccctcttccg tcgccgctca ggtagataac ccaaaagcga tcgtcgtatt
     3421 cgcggctcaa gtgttccgcg taatcttcaa gttgtctgac ctgctcgccg gcatgcggt
//
LOCUS       DASSNN010000651         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1623563, whole genome shotgun sequence.
ACCESSION   DASSNN010000651 DASSNN010000000
VERSION     DASSNN010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623563"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..2555)
                     /locus_tag="VFL84_12125"
     CDS             complement(63..2555)
                     /locus_tag="VFL84_12125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7609418.1"
                     /translation="MNRAPLLSAFGRDLKYAARVLGRSPAFTATAILTLALAIGANTA
                     VFSLVDAILVKPLPYPEPDRLGYVIAPIRTTEGEYVVDSHNGTTWERLRDNVPAIDAA
                     VTRARSGGQQVNLVVGDSAASVRHARVGSGYFRVLGVAPLAGREFTQEEDRPGGPAVT
                     VLSYGLWQRLFNGDPNAVGKTLLLRGEPHEVVGVMPRELRGEGGDFDVYTPVRPSPTG
                     EGAGLNYGVIARVKPGYTWAEALAAMPALDEEYFSRLMGRNWADAQPSGRFSLVPMQQ
                     ALTADAAEPLMTLFGAVCAVLLVACVNLAALLFARATGRAKEIATRLALGSGRGAVVR
                     QLFAESLVLGALGGACGLLLSFACLEALKLFGATTYTEWAGVTLDLRALAVTVAVALG
                     ASVLFGLFPAFAATAVDVNESLKEGGERGNVRGSRHWTRRSLVAAEMALGAVLLIATG
                     LLVRTFVNVSGLEPGFDAAGVTTASVSLQDARYDTAAKMNRLFDESLRRIESSPGVES
                     AAVSLELPFRRLLNAAFSFVDETDPARRAANVTYATPAYFETLRIPLLAGRTIAETDT
                     ASSPAVVTVSQDFVDFLTNGQSPVGRRIRVAGAEREIVGVTGNVKITDTGGIYVPGMI
                     EGPIKSAPLIYLPAAQTPDGLLAVHNTFSPMWSVRAASGAAAETALRSAIASVDPLLP
                     VGAVRPLAELRTAATAKQQLMMVLVGVIGAAALLLSAVGVYGLIAHSVAERRRELGVR
                     LALGATTGRAIRNVAWSGIGLAALGAAAGVVMAWPVAQALDGRGLLWGVDSHDPATFV
                     AGVAFLLVVAAIASIVPALKILRLDPAQALRE"
     gene            2699..>3299
                     /locus_tag="VFL84_12130"
     CDS             2699..>3299
                     /locus_tag="VFL84_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609419.1"
                     /translation="MKTMKLALVPLALAALAGSAGAQQPQQQPANQPPPQPMSFFVTS
                     VGKGDGANLGGLAGADAHCQSLAAAVGRGNVTWRAYMSAGAQGPNGIVHARDRIGRGP
                     WFNARGALIASNVDDLHQDRNNIRKPTALNEKGEEVNGVGNQPNMHDMLTGSDSTGRL
                     VPGNVALTTCNNWTSNSTGNAMLGHHDRLGGPNASWNSVH"
BASE COUNT          565 a         1131 c         1126 g          477 t
ORIGIN      
        1 agttatatag tacgacagac gtagagatac aactcgtctt ttctcaagaa cgccgaatgt
       61 cgttattcgc gcagggcttg cgccggatcg agccgcagaa tcttgagcgc cggaacgatg
      121 ctcgcgatcg ccgcgacgac gagcagaaag gcgacgccgg cgacgaacgt cgcggggtcg
      181 tggctgtcga cgccccagag caatcctctg ccgtcgagcg cttgcgccac cggccaggcc
      241 attacgaccc cggcggcggc gccgagcgcc gcaaggccga tgcccgacca cgcgacgttg
      301 cgtatggctc gacccgtcgt ggcgccgagc gccaagcgca cgccgagctc gcggcgccgc
      361 tcggcgacgc tgtgcgcgat gagcccgtag acgccgacgg ctgagagcag taatgccgcg
      421 gcgccgatga cgccgacgag caccatcatg agctgttgtt tcgcggtcgc cgcggtgcgc
      481 aactccgcga gcggacggac ggcgccgacc ggcagcagcg gatcgacgga cgctatcgcg
      541 ctccgtaacg ccgtttccgc ggcggcgccg ctcgcggctc gcacgctcca catcggcgag
      601 aacgtgttgt gaacggcgag caagccgtcg ggcgtctgcg ccgccggcag gtagatgagt
      661 ggcgcgctct tgatggggcc ttcgatcatg ccaggcacgt agattccgcc cgtgtccgtg
      721 atcttgacgt tgcccgtgac gccgacgatc tcgcgctcgg cgccggcgac gcggatccgg
      781 cgcccgaccg gactctgccc gttcgtcagg aagtcgacga agtcttggct gacggtgacg
      841 acggccggac tcgatgcggt atctgtctcg gcgatggttc ggccagcgag cagcgggatg
      901 cgcagcgtct cgaagtacgc cggcgtggca taggtgacgt tggccgcgcg acgcgctgga
      961 tcggtttcgt cgacgaacga aaaagccgcg tttagcaacc gcctgaaggg aagctcgagc
     1021 gagaccgcgg ccgactcgac gccgggcgac gattcgatgc gccgcaacga ctcgtcgaac
     1081 aggcgattca ttttcgcggc cgtgtcgtag cgcgcgtcct gcagcgacac gctcgccgtc
     1141 gtcacgcccg cggcatcgaa ccccggctcg aggccgctca cgttcacgaa cgttcgcacg
     1201 agaaggcccg tggcgatcaa cagcacggcg ccgagcgcca tctccgcggc tacgagcgag
     1261 cggcgcgtcc agtggcgcga gccgcggaca ttgccgcgct cgcctccttc cttcagcgat
     1321 tcgttgacgt cgacggccgt ggcggcaaac gccgggaaca agccgaatag cacgctcgcg
     1381 ccgagcgcga cggctaccgt tacggcgagc gcgcgcagat cgagcgtgac cccggcccac
     1441 tcggtgtacg tcgtcgcacc gaatagcttc agcgcctcga gacaggcgaa gctcaggagc
     1501 aaaccgcacg caccgccgag cgcgccgagc acgaggctct cggcgaagag ctgccgcacg
     1561 acggcgccgc gtccgctgcc gagcgcgagg cgcgtggcga tttccttcgc gcggccggtc
     1621 gcgcgcgcga agagcagggc ggcgaggttc acgcacgcga cgagcagcac cgcgcacact
     1681 gcgccgaaca gcgtcatgag cggttctgcc gcgtctgcgg tcagcgcttg ctgcatcggc
     1741 acgagcgaga agcggccgct cggctgcgcg tcggcccagt tgcggcccat gaggcggctg
     1801 aaatactcct cgtcgagcgc gggcatcgcc gcgagcgcct ccgcccacgt gtagccgggc
     1861 ttcacgcgcg cgatgacgcc gtagttcaag ccggctccct cgccggtcgg cgagggccgc
     1921 acgggcgtat agacgtcgaa gtcgccgcct tcaccgcgca gctcgcgagg catcacgccg
     1981 acgacctcat gcggctcgcc gcgcagcagc agcgtctttc cgacggcgtt ggggtcgccg
     2041 ttgaagagcc gctgccagag tccgtagctc aacaccgtca cggcggggcc gccgggccga
     2101 tcttcttctt gcgtgaactc gcgcccggcg agcggcgcga caccgagcac acggaagtat
     2161 cccgagccga cccgcgcgtg ccgcaccgac gccgcggagt cgccgacgac gaggttgacc
     2221 tgctgcccgc cggatcgggc tctcgtcacc gccgcatcga tcgcgggcac gttgtcgcgc
     2281 aaccgctccc acgtggtgcc gttgtgcgag tcgacgacgt attcgccttc cgtggttctg
     2341 atcggcgcaa tgacgtatcc caggcgatcc ggctcgggat agggcaacgg cttgaccaga
     2401 atcgcatcga cgaggctgaa gacggccgtg ttcgcgccga ttgcgagcgc gagcgtgagg
     2461 atggctgtcg cggtgaatgc cggtgagcgg ccgagcacgc gcgccgcata tttgaggtcg
     2521 cgtccgaatg cgctgagcag aggtgcccgg ttcatgtcgt cccccgtgcc gtcgccggcg
     2581 agcaaagcat gccaccttca ggttccttca gatagcggaa cgacttcggc ggtttagaat
     2641 cgaggcagcg cgggtccggc cgcgcgcgcg ctcgaacaac aacaccttgg gggaaccgat
     2701 gaaaaccatg aagcttgctc tcgttccgct cgctttggcg gcgctcgccg gctcggccgg
     2761 cgcgcaacaa ccacaacagc aaccggcgaa ccaacctccg ccgcaaccga tgagtttctt
     2821 cgtgacgagt gtagggaagg gcgacggcgc caatctcggc ggcctggccg gcgccgacgc
     2881 gcattgccag tcgcttgcgg ccgctgtcgg ccgcggcaat gtcacgtggc gcgcgtatat
     2941 gagcgccggc gcacagggcc cgaacggcat cgtgcacgcg cgggaccgca tcggccgcgg
     3001 gccgtggttc aacgcgcgcg gcgcgctgat cgcgtcgaac gtcgacgact tgcatcagga
     3061 ccgcaacaac atccgcaagc cgaccgcgct gaacgagaag ggcgaagagg tcaacggcgt
     3121 cggcaaccag ccgaacatgc acgacatgtt gacgggctcc gactcgacgg gtcgcctcgt
     3181 gccgggcaac gtcgcgctga cgacgtgcaa caactggacg agcaacagca cgggcaacgc
     3241 gatgctcggc catcacgacc ggctcggcgg cccgaacgca tcgtggaact ccgtccaca
//
LOCUS       DASSNN010000652         6692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1122812, whole genome shotgun sequence.
ACCESSION   DASSNN010000652 DASSNN010000000
VERSION     DASSNN010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122812"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..858)
                     /locus_tag="VFL84_12135"
     CDS             complement(55..858)
                     /locus_tag="VFL84_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7609420.1"
                     /translation="MTLAATNLEISYGSRTAVRATTVALEPGRLVALVGPNGAGKSTL
                     LRALAGLVAHGGIVTWQDAPLAKLDGRTRARTVAYLPQDPPVHWPLAARELVALGRLP
                     HRAYASDESAADAAAVTTAMRQTDTLEFAERSVSRLSVGERARVLLARALAVEAPVLL
                     ADEPIAMLDPYHQLDVLGTLRSYAAGGESGRNRRVVVAVLHDLGLAARFCDRVLLMDG
                     GAIVGDGAPDATLGAQAIRAHYRVEPLIARHEGEPLIVPWRSLNGRRRD"
     gene            complement(863..1858)
                     /locus_tag="VFL84_12140"
     CDS             complement(863..1858)
                     /locus_tag="VFL84_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET7609421.1"
                     /translation="MSETARSGPTPALLIGLALLTLLAAAASLVIGRDGGGPTFGFGG
                     PELADTIVREIRLPRTLLALLVGGTLGLCGAALQGLLRNPLAEPGLLGASSGAAFGAV
                     IVFYFGFAGASSLKLPLGAVGGSLLALAALYALAGRRGDFLTIVLAGIAINALAAALT
                     SLALNFAPSPYAALEILFWMLGSLADRSLAHVYLAVPLMVPGWLLVVAAARGLDALTL
                     GEDTAFSLGFDPKRTQALVIGGTALAVGGAVAVTGVVGFVGLVVPHLLRRAVGQHPSR
                     LLLPSFLGGALLTTLADVAIRLLPPGPELKLGVVTALLGAPFFLHLVMRSSAREA"
     gene            complement(1855..2709)
                     /locus_tag="VFL84_12145"
     CDS             complement(1855..2709)
                     /locus_tag="VFL84_12145"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7609422.1"
                     /translation="MQRSISRGPRARAPLAAAALAAAAAAGGATAEPLPTVASINLCA
                     DQHLLALADPEQILTVSWLAADPEESMLASEARRHTLNYGTAEELLKFTPDVVLAGTY
                     TSPFTRTMLRRLRYRVVELEPEASIADIERNVRLVAQVVGQVERGEQLVAALRDEVHA
                     IEAKRPARNPAAVIVRPGGFTVGADSLANELLTLAGFRNVAAERGLDRWGSLSMEALL
                     RSAPDVIVLTGYRSAQPSLANAVLEHPALAALGATQRTVTVQTALWACGLPGSIEAAA
                     MLQRAALP"
     regulatory      complement(2828..3009)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3077..4867
                     /locus_tag="VFL84_12150"
     CDS             3077..4867
                     /locus_tag="VFL84_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7609423.1"
                     /translation="MRYRVRIVDLDRHWFEIECRIEDAAAEQRFSMPAWIPGSYLLRD
                     FARHVVRAEAKSGEAPLDVVKTGAAEWCVRGAARALSFTITVYALDQSVRGAYLDRQR
                     GFFNGPCVFVLPDGREAESIEVELERPQQPACAEWRVATALTADEVDERGFGTYRAGD
                     YDELLDHPVELSDFESVEFEAGGVPHRLVIAGRFESDLDRIAADLAQICTAQIEFFGK
                     PPPFERYLFLGLAVGEGHGGLEHRASASLIFGRDDLPKPGETGPSRDYQRFLALASHE
                     YFHTWHVKRTKPAAFMPYRLDRRNHTRLLWVFEGITSYYQDLMLLRSGVIGVNAYLTR
                     LGEILTRVFRMPGRRKQSLEASSFDAWDVLYRPEPHHPNTTISYYTKGALVALALDLT
                     LRLGTAGRTSLDDVMRELWRRHGARGVGVPEDGFERLAAAVSGLDLGSFFASAVRGTD
                     DLPLKELLAEFGVALEMRAAAGADDRGGTPRAANGELLTLGAQVRELAHGLELTSVLE
                     GGAAERAGLNPGDVLVAVDRLRVTGRNLARRLARFESGERVTATVFRGDELLEVGVVL
                     RAAPLDTCYLTVRDPADQAAVERRRAWLGE"
     gene            4880..5572
                     /gene="plsY"
                     /locus_tag="VFL84_12155"
     CDS             4880..5572
                     /gene="plsY"
                     /locus_tag="VFL84_12155"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET7609424.1"
                     /translation="MAETARVSAGRERMIELGLKATLAYLVGSVLGSLLVGFFYGGVD
                     IRKAGSGNAGGTNALRTQGKVFALWVMIVDIGKGILAAIAIPPLVIPGVGIDASVDRS
                     LVLYAVSFAAIVGHVFPVWFGFRGGKGGATAAGLVCYLAPDAGLVMLGAWLLIAFTTG
                     FVGLATIGAALVAVVYLGFTTLPEQSGLFVFACATAALVIYAHRSNIRRMLDGTESRF
                     SSPAARWLRRGQ"
     gene            5579..6565
                     /locus_tag="VFL84_12160"
     CDS             5579..6565
                     /locus_tag="VFL84_12160"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET7609425.1"
                     /translation="MTVEQLLHALADGRTHSGEELARSFGVTRAAIWKQVAKLEDFGL
                     AVDAAPGAGYRLAHRLDLLDAEGVRAALEPAVAARIAKLEVFTELDSTNRRLLASPPR
                     IGALDVCIAEFQTAGRGRRGRRWQAPLGSGIALSVGWQFAGVPAEPSALTLAVGVAVR
                     RVLERVAGLTISLKWPNDLVFDERKLGGILLELAAEAHGGAHVVVGVGLNVALPAALL
                     RTLCDWPRGAVDVATALGRPPPPRAVIAAALVNELAELFADYPTLGFAAYRTEWRSAD
                     YLRGRAVRLEESTGRSFGTALGIDADGALLVETQAGKRRRVVAGDVSVRSVA"
BASE COUNT          951 a         2331 c         2409 g         1001 t
ORIGIN      
        1 ccggctcagg gtcacgaggt tcgccataga ggcgattata cgagggcttg ggcgtcagtc
       61 gcggcgccgt ccgttcagcg agcgccaggg cacgatcaac ggctcgccct cgtggcgcgc
      121 aatgagcggc tccacgcgat agtgcgcgcg gattgcctgc gccccgagcg tagcatcggg
      181 cgctccgtcg ccgacgatgg cgccgccgtc catgagcagc actcggtcgc agaagcgcgc
      241 ggcgaggccg aggtcgtgca ggacggcgac gacgacccgc cgattccggc ccgactcgcc
      301 gccggccgcg tacgagcgca gcgtgccgag cacgtcgagc tgatggtaag gatcgagcat
      361 cgcgatcggc tcgtcggcca ggagcaccgg ggcttcgacg gccagagcgc gcgcgagcag
      421 cacgcgcgcg cgctcgccga ccgaaagccg gctcacgctg cgctccgcga attcgagcgt
      481 gtcggtctgg cgcatcgccg tcgtcacggc ggccgcgtct gcggcgctct cgtcggatgc
      541 gtacgcgcgg tgcggcaacc ggccgagagc gacgagctcg cgagccgcga gcggccaatg
      601 aaccggcggg tcctgcggca ggtacgcgac cgtgcgcgcg cgcgttctgc cgtcgagctt
      661 cgcgagcgga gcgtcttgcc aagtcacgat gccgccgtgc gcgacgaggc cggccagcgc
      721 gcgcagcagc gtgctcttgc ctgccccgtt cgggccgacg agcgcgacga gcctgccggg
      781 ctcgagtgca accgtcgtcg ctcgcacggc cgttcgcgag ccgtagctga tttcgaggtt
      841 cgttgcagca agcgtcatgg tgtcacgctt cgcgcgccga gctgcgcatc acgagatgca
      901 ggaagaacgg cgcgccgagc agcgcggtca cgacgccgag cttgagctcg ggccccggcg
      961 gcagcagccg gatcgcgaca tcggccaacg tggtgagcaa tgcgccgccc aggaagctcg
     1021 gcagcagcag ccggctcggg tgctggccca ccgcgcggcg caacaggtgc gggacgacga
     1081 ggccgacgaa gccgacgacg ccggtcacgg cgacggcgcc gccgacggcg agcgcagtgc
     1141 cgccgatcac gagcgcttgc gtgcgcttcg ggtcgaagcc gaggctgaac gcggtgtcct
     1201 cgccgagcgt cagcgcgtcg agcccgcgcg ccgcggcgac gacgagcagc cagccgggca
     1261 ccatgagcgg cacggcgaga tatacgtgcg cgaggctgcg gtcggcgagc gaaccgagca
     1321 tccagaaaag gatctcgagt gccgcatacg ggctcggggc gaagttcagc gcgagcgagg
     1381 tcaacgcggc ggcgagggcg ttgatcgcga tgcccgcgag cacgatcgtc aagaagtcgc
     1441 cgcgccgccc cgcgagcgcg tagagcgcag cgagcgcgag cagcgagccg ccgacggcgc
     1501 cgagcgggag cttgagcgac gacgcgcccg cgaagccgaa atagaacacg atgacggcgc
     1561 cgaatgcggc gccgctcgac gcgccgagca ggcccggctc ggcgagcgga ttgcggagca
     1621 ggccttgcag cgccgcgccg cacaagccga gcgtgccgcc gacgagcagt gcgagcagcg
     1681 tgcgcggcaa gcggatctcg cgaacgatcg tgtcggcgag ctcgggtccg ccgaagccga
     1741 aggtcggccc gccgccgtcg cgaccgatga cgagcgacgc cgccgctgcc aacagcgtca
     1801 gcaacgcgag cccgatcagg agcgccggcg tcggcccact gcgcgccgtt tcgctcatgg
     1861 cagcgcggcc cgctgcagca tcgccgcggc ctcgatgctg cccggcaagc cgcatgccca
     1921 cagcgcggtc tgcaccgtga cggttcgctg cgtcgcgccg agcgccgcca gcgccggatg
     1981 ctcgagcacg gcgtttgcga gcgacggctg cgcgcttcga tagccggtca gcacgatgac
     2041 gtccggcgcg ctgcgcagca gcgcctccat cgacaagctg ccccaccggt cgaggcctcg
     2101 ctcggccgcg acgttcctga aacccgcgag cgtgagcagc tcgttcgcga gcgagtccgc
     2161 gccgaccgtg aagccgccgg gccgaacgat gacggctgcg ggattccgcg cgggccgctt
     2221 cgcttcaatc gcatgaacct cgtcgcgcag cgccgcgacg agctgctcgc cgcgctcgac
     2281 ttggccgacg acttgcgcga cgagccgcac gttgcgctcg atgtccgcga tcgacgcttc
     2341 gggctcgagc tcgacgacgc gatagcgcag gcgccgcagc atcgtgcgcg tgaacgggct
     2401 cgtgtacgtg ccggcgagca cgacgtcggg cgtgaacttc aggagctcct cggccgtgcc
     2461 atagttcaac gtgtggcgcc gcgcctcgct cgcgagcatc gattcctcgg ggtccgcggc
     2521 gagccagctg acggtcagaa tctgctcggg atcggcaagc gcgagcagat gctgatcggc
     2581 gcacaagttg atcgaggcga cggtcggcag tggctcggcg gtggcgccgc cggctgcggc
     2641 tgccgcggcc agcgccgcgg cggcgagcgg cgcgcgcgca cgcggccctc tcgagatcga
     2701 tcgttgcaca gcgtggctcc gtgtttgccc ggagccgatc cgccgattcg agacggaacc
     2761 tgggcgccga gccgagtctc aacacgtcgc cacgacggaa ctccgtatcg cgcgcactcc
     2821 cggcacacga aaggacgaca gcggcggcag gtctcctggc tcgccggctc gagcggtctc
     2881 gcaagccttc ccgagggttt tacctcagtg gccgtagcgc gagacctcgc cggctacagt
     2941 tgcgggaaca gccgcagtgg tctgcgttcc ctcttagccc gttagggcac cgcctggggc
     3001 caaccttagc acagcgcggc tatgcgacaa tgccgcccac ttcggccgcg ccgcatattc
     3061 gaggacccgc gctctgatcc gctatcgcgt tcgcatcgtc gacctggatc ggcactggtt
     3121 cgagatcgaa tgccgcatcg aggacgcggc tgcggagcaa cgcttctcga tgccggcctg
     3181 gattcccggc agctatttgc tgcgcgactt cgcgaggcac gtcgtgcgcg cggaggcgaa
     3241 gagcggcgaa gcgccgctcg acgtggtcaa gaccggcgcc gcggagtggt gcgtgcgcgg
     3301 cgcggcacgc gcgctctcgt tcacgatcac ggtctacgcg ctcgaccaat ccgtgcgcgg
     3361 cgcgtatctc gatcggcaac gcggcttctt caacggtccg tgcgtgttcg tcttgcccga
     3421 cggccgcgag gcggagtcga tcgaggtcga gctcgagcgg ccgcagcagc cggcctgcgc
     3481 tgagtggcga gtcgcgacgg cgctgaccgc ggacgaggtc gacgagcgcg gcttcggcac
     3541 gtatcgcgcc ggcgattacg acgagctgct cgaccatccc gtcgagctca gcgacttcga
     3601 gagcgtcgag ttcgaagccg gcggcgtgcc gcatcggctc gtcatcgccg gacggttcga
     3661 gtccgacctc gaccgaatcg ccgcggatct cgcgcagatc tgcacggcgc agatcgagtt
     3721 tttcggcaag ccgccgccgt tcgaacgtta tctcttcttg ggcctcgcgg ttggggaagg
     3781 tcacggcggc ctcgagcatc gcgcgtcggc gagcctgatc ttcggccgcg acgacttgcc
     3841 gaagccgggc gagaccgggc cgtcgcgcga ttaccagcgg tttttggcgc tcgcgagcca
     3901 cgagtacttt cacacttggc acgtgaagcg cacgaagccg gccgcgttca tgccgtatcg
     3961 gctcgatcgg cgcaatcaca cgcgccttct ctgggtcttc gaaggcatca cgagctacta
     4021 ccaagacttg atgctgctgc gcagcggcgt gatcggcgtc aacgcgtatc tcacgcggtt
     4081 gggcgagatc ttgacgcgcg tgttccggat gccgggccgg aggaagcaga gtctcgaggc
     4141 gtcgagcttc gacgcgtggg acgtgctcta tcggcccgag ccccatcatc cgaacacgac
     4201 gatcagctat tacacgaagg gcgcgctcgt cgcgctcgcg ctcgacctta cgctgcggct
     4261 cggcaccgcg ggccgaacgt cgctcgacga cgtgatgcgc gagctatggc gccgccacgg
     4321 cgcgcgcggc gtcggcgttc ccgaggacgg cttcgagcgg ctcgccgcgg cggtcagcgg
     4381 gctcgatctc ggctcgttct tcgcgagcgc cgtgcgcggc accgacgact tgccgttgaa
     4441 agagctgctc gcggaattcg gcgtcgcgct cgagatgcgc gcggcggcgg gcgcggacga
     4501 tcgcggcggc acgccgcgtg ccgcgaacgg cgagctgctg acgctcggtg ctcaggtgcg
     4561 cgagctcgcg cacgggctcg agctcacgag cgtgctcgag ggcggcgcgg ccgagcgcgc
     4621 cgggctcaac cccggcgacg tgctcgtcgc ggtcgaccgg ctgcgcgtca cgggccgcaa
     4681 tctggcccgg cgtctcgccc gcttcgagag cggcgagcgc gtgacggcga ccgtgttccg
     4741 cggcgacgag ctcctcgaag tcggagtcgt gctgagagcc gcgccgctcg atacttgcta
     4801 cctgaccgtg cgcgacccgg ccgatcaggc ggccgtcgag cggcggcgag cgtggctcgg
     4861 cgaatgacgg cgccttacca tggcggaaac ggcgcgtgtt tccgcaggta gagagcgcat
     4921 gatcgagctc gggctcaaag cgacgttggc ctatctcgtc ggctccgttc tcggcagcct
     4981 cctcgtcggc tttttctacg gcggggtcga catccgcaaa gccggcagcg gcaacgcagg
     5041 cgggacgaac gcgctgcgca cgcagggcaa agtgtttgcg ctctgggtca tgatcgtcga
     5101 catcggcaag gggattctcg cggcgatcgc gattccgcca ctcgtgattc ccggcgtcgg
     5161 catcgatgcg tcggtcgacc gctcgctcgt gctttacgca gtctcgttcg ccgcgatcgt
     5221 gggccacgtg ttccccgttt ggttcggttt tcgcggcggc aagggcggcg cgacggccgc
     5281 gggtctcgtg tgctatctcg ccccggacgc cgggctcgtc atgctcggcg cgtggctttt
     5341 gatcgcgttc acgacgggct tcgtcggcct cgcgacgatc ggcgccgcgc tcgtcgccgt
     5401 cgtctacctc ggcttcacga ctttgcccga gcagagcggc ctgttcgtgt tcgcttgcgc
     5461 gaccgcagcg ctcgtcatct atgcccaccg cagcaacatc cggcgcatgc tcgacggcac
     5521 cgagtcgcgg ttctcgagcc cggcggcgcg ctggctgcgc cgcggccagt aggcgcgcgt
     5581 gaccgtcgag cagttgctgc acgccttggc cgacggccgg acgcattccg gtgaggagct
     5641 cgcgcggtcg ttcggcgtca cgcgcgccgc gatctggaag caagtggcga agctcgagga
     5701 cttcggcctc gccgtcgatg ccgcacccgg cgccggctac cggctcgcgc atcgtctcga
     5761 ccttctcgat gccgaaggcg tgcgcgcggc gctcgagccc gccgtcgccg cgcgcatcgc
     5821 aaagctcgaa gtgttcaccg agctcgattc gacgaaccgc cgcttgctcg cgtcgccgcc
     5881 gcgaatcggc gcgctcgacg tctgcatcgc ggagtttcag actgcgggtc gcggtcggcg
     5941 gggccggcgt tggcaagcgc cgctcggcag cggcatcgcg ctgtcggtcg gttggcaatt
     6001 cgccggcgtt ccggccgagc cgtcggcgtt gacgctcgcc gtcggcgtcg ccgtgcgccg
     6061 cgtcctcgag cgcgtcgcgg ggctcacgat ttctctgaag tggccgaacg acctcgtgtt
     6121 cgacgaacgc aagctcggcg gcatcttgct cgagctcgcg gccgaggcgc atggcggcgc
     6181 gcacgtcgtc gtgggcgtcg ggctcaacgt cgcgttgccc gccgcgctgc tgcgcacgct
     6241 ttgcgactgg ccgcgcgggg ccgtagacgt tgcgacggcg ctcggtcggc cgccgccgcc
     6301 gcgcgccgtc attgccgctg cgctcgtcaa cgagctcgcg gagctgttcg ccgactaccc
     6361 gaccctgggc ttcgcggcgt atcgcaccga gtggcggtcc gccgattatc tccgcggccg
     6421 cgccgtgcgg ctcgaggagt cgacgggccg ctcgttcggc accgcgctcg gcatcgacgc
     6481 cgacggcgca ttgctcgtcg agacgcaggc cggcaagagg cggcgcgtcg tggccggaga
     6541 cgtcagcgtg cggagcgtcg catgaccgtg ctcgtcgaca tcggcaacac gcgcatcaaa
     6601 tgggcgacgc tctcggccgg caagctcgtg cgccgcggca gcgccgtgca tcgcggcgcg
     6661 ctcgacgacg ccgtcgccgc gttcgcggcg ca
//
LOCUS       DASSNN010000653         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_110512, whole genome shotgun sequence.
ACCESSION   DASSNN010000653 DASSNN010000000
VERSION     DASSNN010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110512"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VFL84_12165"
     CDS             <1..538
                     /locus_tag="VFL84_12165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HET7609426.1"
                     /translation="DVARAICEKMLRRHPHVFGDQKIAGSAEQTKRWEEIKREERGAA
                     DASAGVLDDVPVGLPALTRAVKLGKRAATVGFDWPDVAGVRAKVDEELAELDAAVASG
                     DRDAVAAEMGDLLFSVANWCRHLQLDPESCVRSANERFARRFRAVERAVARDGRGWSD
                     HDAAALDELWRRAKAEEP"
     gene            648..1250
                     /locus_tag="VFL84_12170"
     CDS             648..1250
                     /locus_tag="VFL84_12170"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HET7609427.1"
                     /translation="MLANKFLAVAVLGIVCASPALADHDDRGRGRGNAYGHHKHAPAA
                     VVYAPAVVSARVVNVEPLVRYVTIDRPREQCWNEVVREPAPSLRVVGATAAGSIIGGA
                     IGHQFGSGNDRDALTVLGAVAGGAVAHHRAVAREGYATRDVTVQRCQVVNERVTERVS
                     DGYLVTYRLEGQRYTMRTDRHPGEWVQIAARPVGYRQVRY"
     gene            1343..1708
                     /locus_tag="VFL84_12175"
     CDS             1343..1708
                     /locus_tag="VFL84_12175"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET7609428.1"
                     /translation="MMSHRFTRTIKRAGFLAGIAAAMLLVVDSAGARPRSLALQQAEQ
                     EAEPLGPAQDRLIPAQRGGGISLQEASAKAQSAFPGTVVGARQVQMGDRVVYEIRILG
                     QDGRTVRTFRIDAQTGAFL"
     gene            1720..>2298
                     /locus_tag="VFL84_12180"
     CDS             1720..>2298
                     /locus_tag="VFL84_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7609429.1"
                     /translation="MRLLLVEDDPNLSRSLSAQLEQAGYSVEKSTDGREGLYYAREYP
                     VDLAIVDLGLPSMSGVELIKQLRADGKEYPILVLTARDRWQDKVEALKLGADDYVVKP
                     FNVEELLARVDALLRRAGGWAQSELVCGPITLDTRSQEVKVGGAKLELTSFEYKLLEY
                     LMLHAGEVLSKTQITEALYSEDFERDSNVIEVF"
BASE COUNT          380 a          740 c          814 g          364 t
ORIGIN      
        1 ggacgtcgcg cgcgcaatct gcgagaagat gctgcgccgc catccgcacg tgttcggcga
       61 ccagaaaatc gcgggcagcg cggagcagac gaaacgttgg gaggagatca agcgcgaaga
      121 gcgcggcgcc gccgatgcga gcgcgggcgt gctcgacgac gtgccggtcg ggctgccggc
      181 gctgacgcgc gccgtgaagc tcggcaagcg cgcggcgacc gtcggcttcg actggcccga
      241 cgtcgcgggc gtgcgcgcga aggtcgacga ggagctcgcc gagctcgacg cggcggttgc
      301 gagcggcgac cgcgacgccg tcgccgccga gatgggcgat ctgctgttct ccgtcgcgaa
      361 ttggtgccgg catttgcaac tcgacccgga gagttgcgtg cgctcggcga acgagcgatt
      421 cgcgcgccgt ttccgcgccg tcgagcgcgc cgtggcgcgc gacggccgcg gctggtcgga
      481 tcatgacgcg gccgcgctcg acgaattgtg gcggcgcgcc aaggccgagg agccttgagc
      541 ggcgcggctt tcagccgctt ttcgacattc aggttcgatt cagcgcctcg tccctaacct
      601 gcgctccaag gtcccgagga gggaccgcaa gcaaagggag tgcagccatg ttggcgaaca
      661 aatttctcgc tgttgccgta ctgggcattg tctgcgcgag ccccgcgctg gccgaccacg
      721 atgatcgcgg ccgcggccgc ggtaacgcgt acgggcacca caagcacgcg cccgccgccg
      781 tcgtctacgc gccggccgtc gtctccgcgc gagtcgtcaa cgtggagccg ttggtgcgct
      841 acgtcacgat cgatcgcccg cgcgagcaat gctggaacga ggtcgttcgc gagcccgcgc
      901 cttcccttcg tgtggttggc gcgacggccg cgggcagcat catcgggggc gcgatcggcc
      961 accagttcgg cagcggcaac gaccgcgatg cgctgacggt cctcggcgcg gtggcgggtg
     1021 gtgccgtggc gcatcaccgc gcagtcgcgc gcgaaggcta tgcgacgcgc gacgtcacgg
     1081 tgcagcgctg ccaggtcgtg aacgagcgcg tcaccgagcg agtctccgat gggtatctcg
     1141 tgacgtatcg tctcgagggt cagcgctaca cgatgcggac cgaccgccat cccggcgagt
     1201 gggtgcagat cgccgcgcga cccgtgggtt atcggcaggt gcggtattga gagcgccttc
     1261 cgggcgggcc gttggcccgc ccggttttca ttcgagggtt tccttgacgt ggaccgacgg
     1321 agggcgacaa gctttgccgc caatgatgag ccatcgcttt acaaggacga tcaagcgcgc
     1381 gggtttcctc gcagggatag ctgcggcgat gctgctcgtc gtcgattccg ccggggcgcg
     1441 gccgcgatcg ttggcgctgc agcaagcgga gcaggaagcc gaaccgctcg gcccggcgca
     1501 agacaggctg atacccgccc agcgcggcgg cggcatctcg ctacaagagg cgagcgcgaa
     1561 ggcgcagagc gcgttcccgg gaaccgtcgt gggcgctaga caggtgcaga tgggcgaccg
     1621 cgtcgtttac gagatccgca tcctcggcca agacggacgc acggttcgca cgttccgcat
     1681 cgacgcgcaa accggcgcgt ttctctagag cgcagcgcca tgcgcctgct actagtcgaa
     1741 gacgacccca atctgagccg ttcgctcagc gcccagctcg agcaagcggg ttactccgtc
     1801 gagaagtcga cggatggccg tgaaggcttg tactacgcgc gcgagtaccc cgtcgatctc
     1861 gcgatcgtcg atctcggtct gccgagcatg tcgggcgtcg agctcatcaa gcagctgcga
     1921 gccgatggca aggagtaccc gatcctcgtc ctcacggcgc gcgatcgctg gcaagacaag
     1981 gtcgaggcgt tgaagctcgg cgccgacgac tatgtcgtca agccgttcaa cgtcgaggag
     2041 ctgctcgctc gcgtcgatgc tttgttgcgc cgtgccggag gatgggcgca gtcggagctc
     2101 gtctgcgggc cgatcacgct cgacacgcgg tcgcaagaag tcaaagtcgg cggcgcgaag
     2161 ctcgagctga cgagcttcga atacaagctg ctcgagtacc tcatgctgca cgccggcgag
     2221 gtgctatcga agacgcagat caccgaggcg ctctacagcg aggacttcga gcgcgacagc
     2281 aacgtcatcg aggtcttc
//
LOCUS       DASSNN010000654         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_852628, whole genome shotgun sequence.
ACCESSION   DASSNN010000654 DASSNN010000000
VERSION     DASSNN010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852628"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFL84_12185"
     CDS             complement(<1..216)
                     /locus_tag="VFL84_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET7609430.1"
                     /translation="MGPGTILVGAVVFGVIVLISKSIRVVQQAQTMIVERLGKYHKTL
                     GSGINFIMPVIDKPRAVDWHQTVMTPAG"
     gene            complement(218..661)
                     /locus_tag="VFL84_12190"
     CDS             complement(218..661)
                     /locus_tag="VFL84_12190"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET7609431.1"
                     /translation="MQDWLIWFLAAIALFVAEMFTAGFWLACLAVGAGIAGIAALIPG
                     LGPAVQGVIFAGSSLISMAGLRPRLMQYFQLGPGSDVRTGVDALLGKTAIVTERIEPV
                     TGAGRVKVNGEDWRGASADGATIEPGTKVMVIQVDGTTLMVEKEL"
     gene            731..1795
                     /locus_tag="VFL84_12195"
     CDS             731..1795
                     /locus_tag="VFL84_12195"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET7609432.1"
                     /translation="MLVLLAVLSAVTLQQQAPAPRQALKGDSVAIYNLVGELRVESGS
                     GSDVIVEITRGGADADKLELQTGDVRGRASLRIIYPADVITLPERDRGSQTTLRVRDD
                     GTFNDDSWSGRRYREGREVRIGGRWRRGGSDGLEAYADLRILVPRGKNVAVYLGVGKA
                     TVSTVDGTIKVSVASADVAAERTRGTLRIATGSGNVDLRDGAGEVDLNSGSGDITVAA
                     VQATRLNIETGSGNVTLTDGKATTLSVETGSGDVDVTGAGGDELSFDTGSGNIDIALV
                     TAFNSLNVETGSGDVTLKMPPAIGAQVDIDTGSGDIDLGGFTLAVRRIERDHVTGTLG
                     DGKGRLSVETGSGNVRLLKL"
     gene            complement(1751..2158)
                     /locus_tag="VFL84_12200"
     CDS             complement(1751..2158)
                     /locus_tag="VFL84_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET7609433.1"
                     /translation="MTDNRVRLDRWLWAARFFKTRALSAEAIAGGKVHVNGTRAKAAK
                     QLQVGDELRVRVGPYEWLLTVTGLSERRGPPAVARTLYEESPEGRAARERLAEQHKIA
                     PAPTYQGKGRPTKTERRELLKFEEPDVPRSRFD"
     gene            complement(2155..2493)
                     /locus_tag="VFL84_12205"
     CDS             complement(2155..2493)
                     /locus_tag="VFL84_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET7609434.1"
                     /translation="MWQVAHELTLALYKSTGTFPQAERYGLTSQIRRSASSVCANIAE
                     GCCRQTRREFARFAYVALGSASELEYHLLLAFDLGILKNEQYEVLDGSVTRTKRMLSG
                     LIRKLTTDNR"
BASE COUNT          438 a          822 c          810 g          424 t
ORIGIN      
        1 gcccgcgggc gtcatgacgg tctgatgcca atcgaccgcg cgcggcttgt cgatgaccgg
       61 catgatgaag ttgatgcccg agccgagcgt cttgtggtac ttgcccaggc gctcgacgat
      121 catcgtctgt gcctgctgta ccacgcggat gctcttcgag atcagcacga tgacgccgaa
      181 gacgacggcg ccgacgagta tggtccctgg gcccatctta gagctccttt tccaccatca
      241 gagtcgtccc atccacctga atcaccatga ccttcgtgcc gggctcgatc gtggccccgt
      301 ccgccgacgc gccgcgccag tcctcgccat tcaccttgac ccgcccggcg cccgtcaccg
      361 gctcgatgcg ctcggtcaca atcgccgtct tgccgagcag cgcatccacc ccggtccgca
      421 cgtcggaccc gggaccgagc tggaaatact gcatcagccg gggccggagg cccgccatgc
      481 tgatcaggct cgatcccgcg aaaatgaccc cctggaccgc cggtccgagc ccggggatca
      541 acgccgcgat gccggcgatg cccgcaccga cggccagaca ggccagccag aagccggccg
      601 tgaacatttc tgcgacgaac agggcgatcg ccgcgagaaa ccagatcagc caatcttgca
      661 taaggttcaa agctgcaact ctcgacgcgg tttcgctatc caatcagaga cactcaaata
      721 gggatgcgct atgctcgttc tgttggccgt tctctccgcc gtcacgctcc agcagcaggc
      781 cccggcgccg cggcaggcgc tcaaaggtga ttccgtcgcg atctacaatc tggtgggcga
      841 gctgcgcgtc gagtccggct cgggcagcga cgtgatcgtc gaaatcacgc gcggcggggc
      901 cgatgccgac aagctcgagc tgcaaaccgg cgacgttcgc ggccgcgcgt cgctgcgcat
      961 catctatccc gccgacgtga tcacgctccc cgagcgcgac cgcggctcgc agacgacgct
     1021 gcgcgtgcgc gacgacggca ccttcaacga cgactcgtgg agtgggcggc gctatcgcga
     1081 gggacgcgaa gtgcggatcg gcgggcgctg gcggcgcgga gggagcgacg gactcgaggc
     1141 gtacgcggat ctgcgcatcc tcgtgccgcg cggcaagaac gtcgccgtct acctcggcgt
     1201 cggcaaagcc acggtgtcga ccgtcgacgg cacgatcaag gtgagcgtcg cgtcggcgga
     1261 cgtggcggcc gagcggacgc gcggcacgct gcggatcgcg acgggatcgg ggaacgtcga
     1321 cctgcgcgat ggcgcgggcg aggtcgatct gaacagcggc tcgggcgaca tcacggtcgc
     1381 ggcggtgcag gcgacccggc tcaacatcga gaccggctcc ggcaacgtca cgctgaccga
     1441 cggcaaggcc acgaccctca gtgtcgagac cggctccggg gacgtggacg tcacgggcgc
     1501 gggcggcgac gagctgtcgt tcgacaccgg cagcggcaac atcgacattg cgctcgtgac
     1561 cgccttcaac tcgttgaatg tcgaaaccgg ctcgggcgat gtcacgctga agatgccgcc
     1621 ggccatcggc gcgcaggtgg acatcgatac ggggagcggc gacatcgatc tcggcgggtt
     1681 cacgctggcg gtgcgccgca tcgagcgcga tcacgtcacc ggcacgctcg gcgacggcaa
     1741 ggggcggctc tcagtcgaaa cgggatcggg gaacgtccgg ctcctcaaac tttagcagct
     1801 cgcggcgctc tgttttcgtc ggccggccct tgccttggta ggtgggggcc ggcgcgattt
     1861 tgtgctgctc cgcgagccgc tcgcgcgctg cacggccctc gggcgattcc tcgtacagcg
     1921 tcctggcgac ggcgggcggc ccgcgccgct cggataatcc cgtcacggtg agcagccact
     1981 cgtagggtcc cacgcgcacg cgcagctcgt cgcccacctg gagctgcttc gccgctttgg
     2041 cgcgggtgcc attcacgtgg actttgccgc cggcaatcgc ctcggcagac aaggcgcgcg
     2101 ttttgaagaa gcgcgcggcc cacaaccagc gatcgagacg tactcggttg tcggtcatcg
     2161 gttatcggtt gtcagctttc gaatcaatcc actgagcatg cgcttagttc tggtgacgga
     2221 accgtccagt acttcgtact gctcgttctt taggatgcca agatcgaatg ccaacagaag
     2281 gtggtattcc aattcacttg ccgatcccag tgcaacgtaa gcaaatcggg caaattctcg
     2341 gcgcgtttga cgacagcagc cctctgcgat attcgcgcat actgatgaag cggatcgccg
     2401 aatctgcgaa gtaagtccgt agcgttcggc ttgtggaaac gtaccagtgc tcttgtagag
     2461 agctagtgta agttcgtgag ctacttgcca caca
//
LOCUS       DASSNN010000655         9352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_692791, whole genome shotgun sequence.
ACCESSION   DASSNN010000655 DASSNN010000000
VERSION     DASSNN010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9352
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692791"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2356
                     /gene="alaS"
                     /locus_tag="VFL84_12210"
     CDS             <1..2356
                     /gene="alaS"
                     /locus_tag="VFL84_12210"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET7609435.1"
                     /translation="FFEMLGNFSFGDYFKQEAIQFAWEFVTGTLAIDRKRLMVTVHDS
                     DDEAYDFWHKMIKLPTSKIVRIGNNKPDGGSDNFWQMGDTGPCGPCSEIFYDHGPKVP
                     GGPPGSPDAEGDRWVEIWNLVFMQFDRSADGTLTPLPKPSVDTGAGLERMSAVMQGVT
                     NNYDIDIFVDLIREAARLLGTPDLKNPSLRVIADHIRACSFLIVDGVVPSNEGRGYVL
                     RRIARRAMRHGHKFGVAPTFFNELVPTLSVVMGDAYPELVAKQAFVRDVLRKEGEQFA
                     RTLETGMAILDAAIAKLGGAKVIDGNTVFKLHDTYGFPTDLTADIARERGLRVDMEGY
                     ERAMDVQRRQSQAASQFGADLSAGVKVEGKTEFVGYDALAGEAPVVALLDAAGKPVPA
                     LEKGAEGTVVLESTPFYAESGGQVGDTGELRAGTARFAVSDTQKFGLAFGHRGKLETG
                     RIAVGDRVEAHVDAKRRAAIVLNHSATHLLHAALRKVLGSHVQQKGSLVAPERLRFDF
                     SHYEPVSAEQLAEIEALVNEQIRLNHGAEIRVLPYDEAIAAGALAFFGDKYGDKVRVL
                     KLGEFSTELCGGTHVRRSGDIGLFKIVSEGGIAAGVRRIEAVTGQGALDLVNANEAAL
                     REVAGLVKANKDEVPAKVAQLVERTRSLERELAALRRKLASGGGRDILQEAQVVNGIK
                     VVAARLDGADAKALRDTADQLKSKLGSGVVVLGAVEGDRVFLVASVTQDLAGRLKAGE
                     LIKPVAELVGGRGGGRPDFAQAGGTKPDQVDAALALVPSLIAAA"
     gene            2645..2860
                     /gene="csrA"
                     /locus_tag="VFL84_12215"
     CDS             2645..2860
                     /gene="csrA"
                     /locus_tag="VFL84_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HET7609436.1"
                     /translation="MLILTRRVGETVVIGNDVTVTVLGVKGNQVRLGVNAPREVAVHR
                     EEIFERIKREQVEEKARPPLNGTTVHP"
     gene            2975..3065
                     /locus_tag="VFL84_12220"
     tRNA            2975..3065
                     /locus_tag="VFL84_12220"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3009..3011,aa:Ser,seq:gct)
     gene            complement(3102..3569)
                     /locus_tag="VFL84_12225"
     CDS             complement(3102..3569)
                     /locus_tag="VFL84_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7609437.1"
                     /translation="MYTRILVPVDGSAASARGLDEAIELARHLKARVQLVHVVEPWIV
                     VGAEAPPAAVLQIVESVRSNGAALLKDCEKKVRNAGVEVDRELIETLGSSPGECIVTK
                     ASELNADLIVCGTHGRRGVRRLLMGSDAEYIVRRTPVPVLLVRNQEQAAREAA"
     gene            complement(3723..4937)
                     /locus_tag="VFL84_12230"
     CDS             complement(3723..4937)
                     /locus_tag="VFL84_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609438.1"
                     /translation="MRRHDAGAMSARLWFRALMAVSAALLVVTFASYALVDHRWLARV
                     LFWFGLGGENNIGAWWSGMLLALAAFLAFDGFFDRSKRLVEQRGWLALGCALLLLSFD
                     EIASLHEYLSGLGQSYLAVLGIAGLTLASYAMQQLRRAHVPARTLLKLLIAFGLLATV
                     PIHEAVQQKLQWDNQAVYGLRAFLEEGTEIVAMLIFVAVTRETSASALRSSQDCLVAL
                     VRWPRLIGLTALFLWPVLVAATFELPQPGGPADWLASTLLLACALLAVREALQGGRFD
                     SRSLALILFYAAVSAAANAMKIAWDPVVLGTSVNIRGIAFALLVVTGVLALKAKGRRL
                     RARHAFPVAALIGASAIVWPSSQLVWCAIPPVLAVWFFAIESKAAAESRVALGAGAAP
                     TPEPAPRTKAAA"
     gene            complement(5221..6183)
                     /gene="cysK"
                     /locus_tag="VFL84_12235"
     CDS             complement(5221..6183)
                     /gene="cysK"
                     /locus_tag="VFL84_12235"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET7609439.1"
                     /translation="MIYESILDTIGNTPVVKLHRIPPKHVTIYAKIEAFNPLSSVKDR
                     LAFAIVDDAERRGTLKPGQTVVEATSGNTGIALAMVCAAKGYPFVATMSETFSVERRK
                     IMRALGAKVILTPAAERGTGMVRLAAALAEKHGWFLARQFTNPANPAYHRQTTGPEIL
                     KDFAGRRLDFWVTGWGTGGTLTGAGEVIKLARPEVQIIATEPAGASLLGGKAWAPHKI
                     QGWTPDFLPDVLNPKVFDQLLPVTDEEGKQMARRLAREEGIFVGISAGATMAAAVKAA
                     ETAKPGSVLLAMLPDTGERYLGTYLFDDLNAGSDDEWLASLDKK"
     gene            complement(6180..7646)
                     /gene="cysG"
                     /locus_tag="VFL84_12240"
     CDS             complement(6180..7646)
                     /gene="cysG"
                     /locus_tag="VFL84_12240"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF007922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HET7609440.1"
                     /translation="MQHLPLFADLAQRAVLVVGGGVVAERRVTLLLEAKAAITVVAPE
                     LSPRLVELSAEGAFRHLAKTYAGDPLDAYWLVVAATDDRDVNAAVAAAALAAKRFCNV
                     VDDASSSTFIMPAIVDRSPVTIAIGSSGLSPVLARWIKGVIETLLPARLGALAELAGR
                     WRRRAREQIADAAERRHFWERVVTGVVAEHAFAGRDADAERALEAALASWGSDDVARR
                     GEAYLVGAGPGGIDLITIRGRQLLAAADVVLYDRLVSSELLQYARRDAELVCVGKTPR
                     RPSITQKQLNRLLVSLVRSGKRVCRLKGGDPFVFGRGGEELEALAEAGLKFQVVPGVS
                     AAEGCAAYSGIPLTLRGVAQAVVLTTGHTSADGTPATADYQPGQTLALYMGVAQYGEI
                     AAALLAKGHPSETPVAVVESGTTDRQRVVRTVLGSLGAAQAALDIRPPALLIVGETTR
                     YADRYSWFAPSKVEHFADEPADQGARVSYRQSNHKAST"
     gene            7845..8873
                     /locus_tag="VFL84_12245"
     CDS             7845..8873
                     /locus_tag="VFL84_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET7609441.1"
                     /translation="MTLKQLKYLVGIADSGLNITSAAERLFTSQPGISKQLKQLEAEL
                     GVQLFTRKGKSLAAITPAGREVIARARKILREVGNITSLASDLTAELDGTLSIATTHT
                     QARYVLPEVIRAFRERYPRVDLELHQGTSEQIAELISTNKVDFAIATGSQDLFSHLTL
                     MPIYHWHRIVLVPKGHALTKHTKPLDLKALADHPLVTYVFSGTGESSFKRAFREHGLE
                     PRVVFTARDADIIKTYVRMGMGVGVIASMAFDCADREDLVAIDASELFPRVTTWLGFP
                     RDMVLRGYMVDFIHLFAPHYTPRLIRDAARAATQEEVDEMLHSIALPLKGGCEQEQQR
                     SMRTRVTD"
     gene            complement(9138..>9352)
                     /locus_tag="VFL84_12250"
     CDS             complement(9138..>9352)
                     /locus_tag="VFL84_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HET7609442.1"
                     /translation="HPIAEWTDRDVHRYLTKHNLPYHPLWEKGYVSIGDHHSTKALHE
                     VATVEETRFNGLKRECGIHEINLADL"
BASE COUNT         1604 a         3108 c         3150 g         1490 t
ORIGIN      
        1 gttcttcgag atgctcggca acttctcgtt cggcgattac ttcaagcaag aggcgatcca
       61 gttcgcctgg gaattcgtga ccggcactct cgcgatcgac cgcaagcggc tcatggtcac
      121 ggtccacgac tccgacgacg aggcctacga cttctggcac aagatgatca agctgcctac
      181 gagcaagatc gtccgcatcg gcaacaacaa gccggacggc ggctccgaca atttctggca
      241 gatgggcgac acgggcccgt gcgggccatg cagcgagatc ttctacgacc acggtccaaa
      301 ggtcccgggc ggcccgccgg gctcgccgga cgccgaaggc gatcgctggg tcgagatctg
      361 gaatctcgtg ttcatgcagt tcgaccgctc ggccgacggc acgctgacgc cgttgccgaa
      421 gccgtccgtc gacacgggcg cggggctcga gcgcatgtcg gccgtgatgc agggcgtcac
      481 gaacaactac gacatcgaca ttttcgtcga tctcattcgc gaagcggcgc ggttgctcgg
      541 cacgccagac ctcaagaatc cctcgctgcg cgtcatcgcg gaccacatcc gcgcgtgcag
      601 cttcttgatc gtcgacggcg tcgtgccgtc gaacgaaggc cgcggctacg tgctgcgccg
      661 catcgcgcgg cgcgcaatgc ggcatgggca caagttcggc gtcgcgccca cgttcttcaa
      721 cgagctcgtg ccgacgctta gcgtagtcat gggcgatgcg tatcccgagc tcgtcgcgaa
      781 gcaggcattc gtgcgcgacg tgctgcgcaa ggaaggcgag cagttcgcgc gcacgctcga
      841 gacaggcatg gcgattttgg acgccgcgat cgcgaagctc ggcggcgcca aggtcatcga
      901 cggcaacacc gtcttcaagc tgcacgacac ctacgggttc ccgaccgatc tgacggccga
      961 catcgcgcgc gagcgcgggc tccgcgtcga catggaaggc tacgaacggg ccatggacgt
     1021 gcaacggcgg cagtcgcagg ccgcgagcca gttcggcgcg gacctctccg ccggcgtaaa
     1081 ggtcgagggc aagaccgagt tcgtcggcta cgacgcgctc gcgggcgagg cgcccgtcgt
     1141 cgcgctgctc gatgccgcgg gcaaacctgt gccggcgctc gagaagggcg ccgaaggcac
     1201 cgtcgtcctc gagagcacgc cgttttacgc cgagagcggc ggccaggtcg gcgacacggg
     1261 cgagctgcgc gccggcacgg cccggttcgc cgtcagcgac acgcagaaat tcggcctcgc
     1321 gttcggccat cgcggcaagc tcgagacggg ccgaatcgcc gtcggcgatc gcgtcgaagc
     1381 gcacgtcgat gccaaacggc gcgccgcgat cgtgctgaac cactcggcca cgcatttgct
     1441 gcacgcggcg ctgcgcaaag tgctcgggag ccacgtgcag cagaagggct cgctcgtcgc
     1501 gccggaacgg ctgcgcttcg acttctcgca ctacgagccg gtcagcgcgg agcagctcgc
     1561 cgagatcgag gcgctcgtca acgagcagat tcgcttgaac catggcgccg agatccgcgt
     1621 gctgccgtat gacgaagcga tcgcggccgg cgcgctcgcg ttcttcggcg acaagtacgg
     1681 cgacaaggtc cgcgttctga agctcggcga attctcgacc gagctttgcg gcggcacgca
     1741 cgtcaggcgt tccggcgaca tcggtctgtt caagatcgtc tcggagggcg gcatcgcggc
     1801 cggcgtccgg cgcatcgagg ccgtcacggg gcagggcgcg ctcgacctcg tcaacgcgaa
     1861 cgaggcggcg cttcgcgagg tcgcgggtct cgtcaaggcc aacaaggacg aggtgccggc
     1921 caaggtcgcg cagctcgtcg agcgcacgcg ctcgctcgag cgcgagctcg cggcgctgcg
     1981 ccgcaagctc gcgagcggcg gcgggcgcga catattgcag gaagcgcagg tcgtcaacgg
     2041 catcaaggtc gtggccgcgc ggctcgacgg cgcggacgcg aaggccctgc gcgataccgc
     2101 cgatcagctc aagagcaagc tcggctcggg ggtcgtcgtt ctcggtgccg tcgagggaga
     2161 ccgggtcttt ctcgtagcca gcgtcaccca ggacctggcc gggcgcctga aggccggtga
     2221 gctgatcaag cccgtcgccg agctcgtcgg cggcaggggc ggcggccgtc ccgacttcgc
     2281 gcaggcgggg ggtaccaaac ccgaccaagt cgacgcggcg ctcgcgctcg ttccgagcct
     2341 gatcgcggcc gcctgaccgt ttcctggagc ctgtctaaat ccgggccttt cacaaactcg
     2401 tcactttggc tgttttttcg gctaccatga gccgcgttcg ccagtcgcct tgcgcggggc
     2461 tggtgagcag gggggaccgg cagaacgaca agaacgagcc ggttagccct gaagtctgcc
     2521 ggattcgtcc gtgcgcaagc gcggagggga cgaggtggac taggggcgat tgccgaatcc
     2581 gacaacaaca gctccgctta gcagagcaac ggaccggcga agcagggcct agaaggagtt
     2641 ggctatgctg atcttgaccc ggcgcgtcgg ggaaacggtc gtcatcggta acgatgtcac
     2701 cgtgaccgtc ctgggggtga aaggcaatca agtgcggctc ggcgtgaacg ccccgcgcga
     2761 agtcgccgtc caccgcgaag agatcttcga acgcatcaag cgcgagcaag tcgaagaaaa
     2821 agcgcggcca ccgttgaacg gcaccaccgt tcacccgtag cgatacaccg gtcgctgcgg
     2881 ctcgtcgcat catccgacgt gcgcccgcgc gggcgtcgcg attctgtgtt tccgccagta
     2941 gaattgcagc ctcgcagatt caatccgcgt ccccggagag atggccgagt ggtcgaaggc
     3001 gcacccctgc taagggtgta agggccaaaa gcccttcgag ggttcgaatc cctctctctc
     3061 cgccacttct ttacgggcag ggttgaaggg tcgcggctgg cctacgctgc ctcgcgggcg
     3121 gcctgctcct gattacgcac caacagcact ggcaccggcg tgcggcggac gatgtactcg
     3181 gcgtcgctcc ccattagtag tctgcgaacg ccgcggcgcc cgtgagtgcc gcaaacgatg
     3241 aggtcggcat tgagctcgct ggctttggtc acgatgcatt cgccgggcga gctcccgagc
     3301 gtctcgatca actctctatc gacctctacg ccggcattcc ggaccttctt ttcgcaatcc
     3361 ttgagcagcg cggcgccgtt actgcgtaca gattcgacga tctgaagaac ggcggctggc
     3421 ggcgcttcgg ctcccacgac gatccaaggc tctacgacat gcacgagctg cacgcgggct
     3481 ttcaaatgcc gtgcaagctc aatggcctcg tcgaggcctc gggcggatgc tgcgcttcca
     3541 tcgactggaa cgagaattcg cgtgtacacg acacgacctc ctgctgggct gtagcgctga
     3601 gcacagcaag aattgggccg gtgtcttacg gcaacaaata gtacgtgtgc tcgggatcga
     3661 ggaccggcgg tagcttccgg aacggccgcg gcagaatcaa ttccgcgttc gggcagagac
     3721 ggctatgcgg ctgctttggt tctcggggcc ggttcgggcg tgggcgctgc gcctgcgccg
     3781 agcgcgactc tgctttctgc ggctgccttg ctttcgatcg cgaagaacca taccgccaac
     3841 acgggcggta tcgcgcacca gacgagctgc gacgacggcc acacgatcgc cgaggcgccg
     3901 atgagcgctg cgacgggaaa cgcgtgccgc gcccttaagc ggcgcccctt cgccttgagc
     3961 gcgagaacgc ccgtcacgac tagcagcgcg aacgcgatgc cgcgaatgtt gacgctcgtg
     4021 ccgagcacga cggggtccca tgcgatcttc atggcattcg cggccgcgga cacggccgca
     4081 tagaaaagga tcagcgcgag agagcgcgaa tcgaatcgcc cgccctgcaa cgcctctcga
     4141 acggcgagca gcgcgcaagc caagagcagc gtcgacgcca gccagtcggc gggaccgccg
     4201 ggctgcggca gctcgaacgt cgcagccacc aagaccggcc aaaggaacag cgcggtcaga
     4261 ccgatgagcc gcggccaacg cacgagcgcg acgagacagt cttgcgacga tcgcagcgcc
     4321 gacgccgacg tctcgcgcgt caccgcgacg aagatcagca tggcgacgat ctcggtgcct
     4381 tcttcgagga aggcgcgcag gccgtagacc gcctggttgt cccattgcag cttctgctga
     4441 accgcctcgt ggatcggcac ggtggccagc agcccgaacg cgatcagcag cttgaggagc
     4501 gttcgcgccg gtacgtgcgc gcgacgcaat tgctgcatgg cgtacgaggc gagggtcagg
     4561 ccggcgattc cgagcacggc aaggtaactc tgcccgagcc ccgacaagta ctcgtgcagc
     4621 gacgcgatct cgtcgaacga gagcagcagc agcgcgcaac cgagcgcaag ccagccgcgc
     4681 tgctcgacga gccgcttgct tcgatcgaag aagccgtcga acgccaggaa cgccgcgagc
     4741 gccagcaaca tccccgacca ccacgcgccg atgttgttct cgccgcccag gccgaaccag
     4801 aacaggacgc gcgcaagcca gcgatggtcg acgagggcgt aagaggcgaa cgtcacgacg
     4861 agcagcgctg ccgagactgc catgagcgcg cgaaaccaca gccgtgcgct catggcaccg
     4921 gcatcgtgtc gacgcatggt tgtgcctccc ttcgccgccg gtcgtggctg accgtagccg
     4981 gcgtaacccg gcccgacgcg atgcactttc ggtgccaagg cggttcttcg agcgcgctcg
     5041 ctttggccgt cgccgctttt tttcacgagc ggcaattcac tgccatcgca aattgtggaa
     5101 gttgctccgc ggcggcgcgg cgcggacgaa aaggcgcgtg ctcggagcag gccgtgctcg
     5161 tccgaagaat ttcgaggatc gtctgggctg ggtcccggag ctccgcgctc cgctcgtcgg
     5221 ctacttcttg tcgaggctcg cgagccactc gtcgtccgag cccgcgttca aatcgtcgaa
     5281 gagataggtg cccaagtagc gctcgcccgt gtcgggcagc attgcgagca gcacggagcc
     5341 cggcttcgcg gtctcggcgg ccttgacggc cgcggccatc gtcgcgccgg ccgagatgcc
     5401 gacgaagatg ccttcctcgc gcgcgagacg gcgcgccatc tgcttgcctt cctcgtccgt
     5461 gaccggcaag agctggtcga acactttcgg gttcagcacg tcgggcaaga agtccggcgt
     5521 ccaaccttgg atcttgtgcg gcgcccacgc cttgccgccg agcaatgacg cgccggcagg
     5581 ctccgtcgcg atgatctgca cctcggggcg cgcgagcttg atcacctcgc ccgcacccgt
     5641 cagcgtgccg cccgtgcccc aacccgtcac ccagaagtcg agtctgcggc ccgcgaagtc
     5701 cttcaagatc tcggggcccg tcgtttgccg gtgataggcc gggttcgcgg gattcgtgaa
     5761 ctgccgcgcg aggaaccagc cgtgcttctc ggcgagcgcc gccgccagcc gcaccatgcc
     5821 ggtgccgcgc tccgcggccg gcgtcaggat caccttcgcg ccgagcgcgc gcatgatctt
     5881 gcggcgctcg accgagaacg tctcggacat cgtcgcgacg aacggatagc ccttcgccgc
     5941 gcagaccatc gcgagcgcta tgccggtgtt gccggacgtc gcctcgacga cggtttggcc
     6001 gggcttcagg gtgccgcgcc gctcggcgtc gtcgacgatc gcgaacgcga ggcgatcctt
     6061 gaccgacgag agcggattga acgcctcgat cttcgcgtag atcgtgacgt gcttcggcgg
     6121 gatccgatgc agcttgacga cgggcgtgtt gccgatcgtg tcgaggatgc tttcgtagat
     6181 catgtggaag ccttgtggtt gctttgacgg tagcttacac gagcgccttg atcggccggc
     6241 tcgtcggcga agtgctcgac cttgctcggc gcgaaccagc tgtagcgatc ggcgtagcgc
     6301 gtcgtctcgc cgacgatcag gagcgccggc ggccggatgt cgagcgcggc ctgagcggcg
     6361 cccaagctgc cgagcaccgt tcgcacgacg cgctgccggt cggtcgtgcc gctctcgacg
     6421 acggcgaccg gcgtttcgct tggatggcct ttcgcgagca atgcggcggc gatctcgccg
     6481 tattgcgcca cgcccatgta gagcgcgagc gtctgtccgg gttggtagtc ggccgtcgcc
     6541 ggcgtgccgt cggcgctcgt gtgacccgtc gtcagcacga cggcctgcgc gacgccgcgt
     6601 agcgtcaacg gaatgccgga gtaggcggcg cagccctcgg cggcggagac gccgggaacg
     6661 acttgaaact tgagccccgc ttccgcgagc gcctcgagct cctcgccgcc gcggccgaac
     6721 acgaacggat cgccgccctt gagccggcac acgcgcttgc cggatcgaac gaggctcacg
     6781 agcagccgat tcaattgctt ctgcgtgatc gacggccggc gcggcgtctt gccgacgcag
     6841 acgagctcgg catcacggcg tgcgtattgc aggagctcgg aactgacgag ccggtcatag
     6901 agcacgacgt cggccgcggc caggagctga cggccgcgga tcgtgatgag atcgattccg
     6961 cccggaccgg cgccgacgag atacgcttcg ccgcggcgtg caacatcgtc gctgccccag
     7021 ctcgcaagcg ccgcttcgag cgcgcgctcg gcatcggcgt cgcgccccgc gaacgcatgc
     7081 tcggctacga cgccggtcac gacgcgctcc cagaaatggc gccgctccgc cgcatccgcg
     7141 atctgctcgc gcgcgcgccg gcgccaccgg ccggcgagct ccgccagcgc gccgagccgc
     7201 gccggcagca gcgtttcgat gacgcctttg atccagcgcg cgagcacggg cgacaggccg
     7261 ctgctgccga tcgcgatcgt gaccggcgag cggtcgacga tggccggcat gatgaacgtg
     7321 ctcgaagacg cgtcgtcgac gacgttgcag aagcgtttcg cggctagcgc cgccgcggcg
     7381 accgcggcgt tcacgtcccg atcgtcggtg gcggcgacga ccagccagta ggcgtcgagc
     7441 gggtcgccgg cgtaggtctt tgcgagatgc ctgaacgcgc cctcggccga gagctcgacg
     7501 aggcgcgggg acagctccgg ggccaccacg gttatggcgg ccttggcttc gagcagcagc
     7561 gtcacgcgcc gctcggcgac gacgcctccg ccgacgacga ggacggctcg ctgcgcaagg
     7621 tccgcaaaca ggggtagatg ctgcatggga ataaggagcc tagccgcagc cacgataccg
     7681 cgcccgcgga tcgcccaaca acaccatatc cgcctaggtt tatagcgccc cgcaatatcg
     7741 gcggccgcgt tgacaccccg atctaaccga tggttatatg cagcccagcg gccgccgact
     7801 cgcacgagga agcggccttt tcatctcgat ggggggtctg gcacgtgacg ctgaagcagc
     7861 tcaagtacct cgtcggcatc gccgacagcg gcttgaacat cacgtcggcc gcggagcggc
     7921 tgttcacgag ccagcccggc atcagcaagc agctgaaaca gctcgaggcc gagctcgggg
     7981 tgcagctctt cacgcgcaag ggcaagagct tggcggcgat cacgccggcc ggccgcgagg
     8041 tcatcgcgcg ggcgcgcaaa atcttgcgcg aggtaggcaa catcacgagc ctcgcgagcg
     8101 acctgacggc cgagctcgac ggcacgttgt cgatcgcgac gacgcacacg caagcgcgct
     8161 acgtgctgcc cgaggtgatc cgtgcgttcc gcgagcgcta tccgcgcgtc gacctggagc
     8221 tgcaccaggg gacgtcggag cagatcgccg agctcatcag cacgaacaag gtcgacttcg
     8281 caatcgcgac cggatcgcag gacctgttct cgcacctgac gctgatgccg atttatcact
     8341 ggcatcgcat cgtgctcgtg ccgaagggcc acgcgctgac gaagcatacg aagccgctcg
     8401 acttgaaggc gctcgccgat catccgctcg tgacctacgt gttcagcgga accggcgagt
     8461 cgtcgttcaa gcgcgcgttc cgcgagcacg gcctcgagcc gcgcgtcgta ttcaccgcgc
     8521 gcgacgccga catcatcaag acctacgtgc gcatggggat gggcgtcggc gtcatcgcgt
     8581 cgatggcgtt cgactgcgcg gaccgcgaag atctcgtcgc gatcgacgcg agcgagctgt
     8641 tcccgcgcgt cacgacctgg ctcggctttc cgcgggacat ggtgctgcgc ggctacatgg
     8701 tcgacttcat tcacctgttc gcgccgcact acacgccgcg cctgatccgc gacgccgcgc
     8761 gagcggcgac gcaagaagag gtcgacgaga tgctgcattc gatcgcgttg ccgctgaagg
     8821 gcggctgcga gcaggagcag cagcgcagca tgcggacgcg agtgacggat tgagcgaacg
     8881 taatccttac gcgccaccca ccgcggaggt ccgcgacgcg cctcggccag ccgatggcgc
     8941 aggcaatctg gcgggtatcg ttcggtgaca cgctgctcct gaccctcggc ggcatgacgg
     9001 ccgggcgtcg gcaacgtata cggactggtc gacgcgctgt tcgtctttcg caatgaccgg
     9061 cgctgcgtgc acgacttgat agccggcacg aaagtcatca acgcggagac ggcgtcgaca
     9121 gcgacttcgg tgtcgggcta gaggtcggcg agattgatct cgtggatgcc gcactcgcgc
     9181 ttcaagccgt tgaagcgcgt ctcctcgacg gtagcgactt cgtgcagcgc tttggtgctg
     9241 tgatggtcgc cgatcgagac gtagcctttc tcccagagcg ggtgatacgg caggttgtgc
     9301 ttcgtcaggt agcgatgcac gtcgcggtcg gtccattcgg cgatcgggtg ca
//
LOCUS       DASSNN010000656         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1860042, whole genome shotgun sequence.
ACCESSION   DASSNN010000656 DASSNN010000000
VERSION     DASSNN010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860042"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..783
                     /locus_tag="VFL84_12255"
     CDS             187..783
                     /locus_tag="VFL84_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609443.1"
                     /translation="MVDMRHLKELLLQSLEHERAGVDVYAAAIRCAVSQDLKHQWSEY
                     LEQTQTHVDILENVLRKLEMDPEQLTSGRRIVRSLGNALVMAMETAKATGDPASAQLV
                     ACECIVLAETKDHMNWELLTKGAARLEGAQKQALEEACEIVEEQEDEHLYHSRGYWRE
                     LWLDALGLPAMLPPPEEMQHVRSAISAAQAQQQSERNR"
     gene            803..>2694
                     /locus_tag="VFL84_12260"
     CDS             803..>2694
                     /locus_tag="VFL84_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET7609444.1"
                     /translation="MTARSLVPPGRSQSDRASTSKPLEDYALIGNMISAALVGRDGSI
                     DWLCLPRFDSPACFAALLGDSANGRWIIAPVGAHESKRSYYSGTAVLETRFSTSSGEA
                     ALIDFMPQTDDADKIDVVRIVRGLRGSVEFDMELVLRFAYGTAVPWVQRRDYGLSAIA
                     GPDAVELHTRAPLEGRDLTTHSKFTVREGDSMPFTLSYHRSHEAPHFVPDTRESLNNT
                     VTAWREWSKHCVFKCQRGDWNEAVMRSLITLKLLTYAPTGGIVAAPTTSLPEKLGGPR
                     NWDYRYCWIRDSALTLFALLNAGYREEAERWRRWLLRATAGHPGQLRIMYGISGERLL
                     PEHELPWLPGYEGSSPVRIGNKAASQRQLDVYGELLDVLHTARDADLAPLEDAWALQK
                     TLLRDLEHAWKEHDHGIWETRGPARAFTHSRLMSWVAFDRGIKSCEHFGLDGPVDRWR
                     ATRAEIHDDVCKNAFDAERNTFVQYYGARDLDAALLLIPQTGFLPANDPRFVGTVAAI
                     ERELVVGDGLVMRYTKDIDELGREGAFLLCSFWLADAYVMLGRLEDATRIFDRMLDLR
                     NDLGLLSEEYDPDRKRLIGNFPQGFSHIGLINTAFNLVKAHGPAQQRAQRTAPSDGEG
                     VLKDKER"
BASE COUNT          477 a          882 c          896 g          439 t
ORIGIN      
        1 cgcgttatgc attagcgtgc tcgcggatac gcggacgttt gaggattcga accgatgcct
       61 ttccgcactg agccactcgg ctgctgcggc ggccgagtgg cgcacttttg tgcgcgctcg
      121 atccgtagcg gccggggcac gtcacttgca acgtgaggtt ctcagcggta tctacggagc
      181 acggccatgg tggacatgcg gcacttgaag gagcttctgc tccagtcgct cgagcacgag
      241 cgcgcgggcg tcgacgttta cgcggcggcc atccgctgtg cggtctcgca ggatctcaag
      301 caccaatggt cggaatacct cgagcaaacg caaacccacg tcgacatcct cgagaacgtc
      361 ttgcgcaagc tcgagatgga tccggagcag ctcacgtcgg gccgccgcat cgtccgttcg
      421 ctcggtaacg cgttggtcat ggcaatggag acggcgaagg cgacaggcga cccggcatcc
      481 gctcagctcg tcgcctgcga atgcatcgtc ctcgccgaga cgaaagacca tatgaactgg
      541 gagctgctga cgaagggcgc cgcccgcctc gaaggcgcgc agaagcaagc tctcgaggag
      601 gcctgcgaga tcgtcgagga gcaggaggac gagcacctct accacagccg cggctactgg
      661 cgtgagctgt ggctcgacgc gctcgggctg ccggccatgc tgccgccgcc cgaggaaatg
      721 cagcacgtgc gatcggcgat cagcgcggcg caggctcagc agcaaagcga acgcaatcgc
      781 tgaccggagg cgcgcgccga gcatgacagc gcgatcgctc gtccctccgg ggcgcagtca
      841 gtcggaccgt gcctctacgt ccaagccgct cgaggactac gcgctgatcg gcaacatgat
      901 ctcggcggcg ctggtcggcc gcgacgggtc gatcgattgg ctctgcttgc cgcgattcga
      961 ttcgcccgct tgctttgcgg cgttgctcgg cgacagcgcg aacggccggt ggatcatcgc
     1021 gccggtcggg gcccacgaga gcaaacgttc gtactactcg ggcaccgcgg tgctcgagac
     1081 acgcttttcg acgagcagcg gcgaggcggc gctgatcgac ttcatgccgc agacggacga
     1141 cgcagacaaa atcgacgtcg tgcgcatcgt gcgcggcctt cgcggcagcg tcgaattcga
     1201 catggagctc gtgctgcgct tcgcgtacgg caccgccgtg ccgtgggtcc agcggcgcga
     1261 ctacggcctg agcgcgatcg ccggcccgga cgccgtcgag cttcacacac gcgcgccgct
     1321 cgagggccgc gacttgacga cccactcgaa gttcacggtc cgcgagggcg acagcatgcc
     1381 cttcacgctg tcctaccacc gatcgcacga ggcaccgcat ttcgttccgg acacgcgcga
     1441 gagcctcaac aacaccgtga cggcgtggcg cgaatggtcg aagcactgcg tcttcaaatg
     1501 ccagcgcggc gattggaacg aagccgtgat gcgctcgctg atcacgctga agctgctgac
     1561 ctatgctccg acgggcggaa tcgtcgccgc accgacgacc tcgttgcccg aaaagctcgg
     1621 cggcccgcgc aactgggatt atcgctattg ctggattcgc gactccgccc tgacgctgtt
     1681 cgcgctgctc aacgcgggct atcgcgagga agccgaacgg tggcggcgtt ggctgctgcg
     1741 cgcgacggcc ggtcacccag ggcagctccg gatcatgtac ggcatctccg gcgagcgttt
     1801 gctgcccgag cacgagctgc cttggctccc ggggtacgaa ggcagctcgc ccgttcgcat
     1861 cggcaacaag gcggcgtcgc aacgccagct cgacgtttat ggcgagcttc tcgacgtgct
     1921 gcataccgcg cgcgacgccg acctcgcgcc gctcgaggac gcctgggcct tgcagaagac
     1981 gctgctcagg gacctcgagc acgcgtggaa ggagcacgac catggaatct gggaaacgcg
     2041 cgggccggcg cgcgcgttca cgcattcgcg gctgatgagc tgggtcgcat tcgaccgcgg
     2101 catcaagagc tgcgagcact tcgggctcga cgggccagtc gatcgatggc gcgcaacgcg
     2161 agcggagatc cacgacgacg tttgcaagaa cgcgttcgac gccgagcgca acacgttcgt
     2221 ccagtattac ggggcccgag acctcgacgc cgcgctcttg ctgattccgc agaccgggtt
     2281 tttgcccgcg aacgatccgc gcttcgtcgg cacggtggcg gcgatcgagc gcgagctcgt
     2341 cgtcggcgac ggcctcgtca tgcgctacac gaaagacatc gacgagttgg gacgcgaggg
     2401 cgcgttcttg ctctgctctt tctggctggc ggacgcgtac gtcatgctgg ggcggctcga
     2461 ggacgcgacg cggatcttcg accgcatgct cgatctgcgc aacgacttag ggctactctc
     2521 cgaggagtac gatcccgacc ggaagcggct catcggcaac ttcccgcaag gcttctcgca
     2581 catcggcctg atcaacaccg cattcaacct cgtgaaggcg catggaccgg cgcaacagcg
     2641 tgctcagcgc acggcgccga gcgacggtga gggagttctc aaggacaagg agcg
//
LOCUS       DASSNN010000657         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1243564, whole genome shotgun sequence.
ACCESSION   DASSNN010000657 DASSNN010000000
VERSION     DASSNN010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243564"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFL84_12265"
     CDS             complement(<1..620)
                     /locus_tag="VFL84_12265"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609445.1"
                     /translation="MRLAATLGVVAAGLAARGALAHHSPARYDLETQRTVEGAVTQYE
                     WGNPHVYLFVRENGAERVWTVEAYPSTAMKQYGWAKDTFAVGDRVVVAGNPGRNAAQS
                     TLFLRSIRKAGATAALYDSGGAIAAPPPTPRETFRATSIAGTWVSSVGPVFANFFGPG
                     LAEAATPQGAAAVAEFRDTANPGLECVPFAPPVYMILPGYRSIEIRS"
     gene            complement(529..1863)
                     /locus_tag="VFL84_12270"
     CDS             complement(529..1863)
                     /locus_tag="VFL84_12270"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609446.1"
                     /translation="MNHQSLRIRRRHALGGLLTAGLIVAPAAYAHHGFGNFAMDEDIT
                     LSGDIIKVDFVNPHAWVHFNVTKPDGTKAEYRCELRSATTLRRSGWTPEMFAAGTHIT
                     IQGSPDRAEKNACYTSTITFGDGTVLDRYGQRIPAQVTTQRSARLPSGEPNISGDWAV
                     EQLVMTDPAGRDGTLVPLSRVGQFERGAVPEGQQEIPGARGTPQAQQPRTAPPGGGGG
                     GGFAGRPGPVELTDAGKAAVASLPQMSRAERSCASGSIVSDWGGEPVNRITQGAGTIT
                     IQYGRNGRTRTVHMTGAHPANITPSADGHSIGKWENDVLVVDTIGFIPGTLVGQTPHS
                     AAARRRALLGRSGDDDVEARGAGRRSSLLEDELHEQHHDDRFDRAVRCGSLSGFDAGR
                     AAGAGGGSRARALRAFRARRARSAARGDARRRRGRSRCARRARASQPRALRP"
     gene            2043..>2542
                     /locus_tag="VFL84_12275"
     CDS             2043..>2542
                     /locus_tag="VFL84_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609447.1"
                     /translation="MAALTLGGLAQSGSDEPTAWVTPRTADGQPDLQGHWTNDTYTPL
                     ERPVELGDKALFTPEEAAAFFKSKVDDLNAQAANDIHYDDAIWQAENYDKVASRRTSL
                     IVDPPNGRLPPLTAQGEDRLPRQRATQRATSADSAKSRSLGERCISWGNVGPPMMPPT
                     YNANLQ"
BASE COUNT          402 a          879 c          869 g          392 t
ORIGIN      
        1 gagcgaatct cgatgctgcg atagcccggc agaatcatgt agacgggcgg cgcgaacggc
       61 acgcactcga ggccgggatt cgccgtgtcg cgaaactccg cgacggctgc ggcgccttgc
      121 ggcgtagccg cttccgcgag cccgggcccg aagaaattcg cgaacacggg gccgacgctg
      181 gacacccacg tcccggcgat cgacgtggcc cgaaacgtct cgcgcggtgt cggcggcgga
      241 gccgcaatcg cgccgcccga gtcatagagc gccgcggtag cgccggcttt gcgaatgctg
      301 cgcaggaaca gcgtgctctg cgcggcgttc ctgccgggat tgccggcgac gacgacgcga
      361 tcgccgaccg cgaacgtgtc cttcgcccag ccgtactgct tcatcgccgt cgacggatac
      421 gcttcgaccg tccagacgcg ctcggcgccg ttctcgcgca cgaatagata gacgtgcggg
      481 ttgccccact cgtactgcgt gaccgcgccc tcgacggtgc gttgcgtctc aaggtcgtag
      541 cgcgcggggc tgtgatgcgc gagcgcgccg cgcgcagcga gaccggccgc gacgacgccg
      601 agcgtcgccg cgagccgcac tgcgcgcgcg cctcgcacga aacgctctca gcgcgcgggc
      661 ccgggagccg ccgccggcgc cggcggcacg accggcgtca aatcctgaca agcttccgca
      721 gcgtacggca cggtcgaaac gatcatcgtg gtgctgttcg tgaagctcgt cttccagtag
      781 agaggatcgt cggcctgcac ctcgcgcttc aacgtcgtcg tcgccggatc gaccgagaag
      841 cgctcgacga cgtgcagctg cgctgtgcgg agtttggccg accagcgtgc cgggaatgaa
      901 gccgatcgtg tcgaccacga gcacgtcgtt ctcccacttg ccgatcgagt ggccgtcggc
      961 gctcggcgtg atgttcgcgg gatgcgcgcc cgtcatgtgc acggtgcgcg ttcggccgtt
     1021 ccgcccgtac tggatcgtga tcgtgcccgc gccttgcgtg atgcgattga cgggctcgcc
     1081 gccccaatcc gagacgatgc tgccggacgc gcacgagcgc tccgcgcgcg acatctgcgg
     1141 caacgacgcg accgccgcct tgccggcgtc cgtcagctcg acaggcccgg gccgacccgc
     1201 gaagccgccg ccgccaccgc ctccgggcgg cgccgtgcgc ggctgttgcg cctgcggcgt
     1261 accgcgcgcg cccggaattt cttgctggcc ttcgggcacg gcgccgcgct cgaattgtcc
     1321 gacgcgcgag agcggcacga gcgtgccgtc tcggccggcc gggtccgtca tgacgagctg
     1381 ctcgacggcc cagtcgccgg aaatgttcgg ctcgccgctc ggcaggcgcg ccgagcgctg
     1441 cgtcgtgacc tgcgccggaa tgcgctggcc gtagcgatcg agcacggtgc cgtcgccgaa
     1501 cgtgatcgtg ctcgtgtagc acgcgttctt ctcggcccga tccggcgagc cttggatcgt
     1561 gatgtgcgtg ccggccgcga acatctcggg cgtccagccc gagcggcgca gcgttgtcgc
     1621 ggagcgcagc tcgcagcggt attcggcctt cgtgccgtcc ggtttcgtga cgttgaagtg
     1681 cacccacgcg tgcgggttca cgaagtcgac cttgatgatg tcgcccgaga gcgtgatgtc
     1741 ctcgtccatc gcgaagttgc cgaagccgtg atgggcatag gcagcgggcg cgacgatgag
     1801 acccgccgtg agcaggccac cgagggcatg ccggcgccga atacgaagtg actgatgatt
     1861 cacgctattc ccccaatagt tcgcgtgtag attgggcgca ttatccgtgc ggcgcggcga
     1921 atcgtcgatg acctgaagga aagttgactg gccgggccgg cgcgcctagc gttgagcggt
     1981 cgcaagttcc ggccggtggc gccatgaaac gtcgcattcg gtccctcgga gcagccgcag
     2041 ctttggccgc cctgacactc ggcggcctcg cacaatcggg ctcggatgag ccgacggcgt
     2101 gggtcacacc gcgtacagcc gacggtcagc ccgacctgca aggtcactgg acgaacgaca
     2161 cttacacgcc gctcgagcgc cccgtcgagc tcggcgacaa ggcgctgttc acgccggaag
     2221 aagccgcggc gttcttcaag tcgaaagtcg acgacttgaa cgcgcaggcc gcgaacgaca
     2281 tccactacga cgacgcgatc tggcaagccg agaactacga caaggtcgcg agccggcgca
     2341 cctcgttgat cgtcgatccg ccgaacggcc gtttaccgcc gttgacggcg caaggcgaag
     2401 accggctgcc tcggcaacgc gcgacgcagc gcgcaacctc cgccgacagc gccaagagcc
     2461 gctcgctcgg cgaacggtgc atctcgtggg gcaacgtcgg cccgccgatg atgccgccga
     2521 cctacaacgc gaacttgcag at
//
LOCUS       DASSNN010000658         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_975819, whole genome shotgun sequence.
ACCESSION   DASSNN010000658 DASSNN010000000
VERSION     DASSNN010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975819"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFL84_12280"
     CDS             <1..788
                     /locus_tag="VFL84_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7609448.1"
                     /translation="ALYNRQFDTAVALIEHGADVDQWDWWGRSPLYLAIELNRIPDSR
                     RGDLPALDKNTGLDVARLLLERGANVNMRLKHQPPLRNEPGDRGYVDGGPDVLVVSPG
                     ATALHVAAKASDDDAVKLLLAHGANVQAANVFGITPVMAAAGVGHWYGVFREFPTIGR
                     YKTGADAVATMKLLLAAGARLDGRTTELSIGFQRPRIAGLTAAHGAAFQGWNEVIEFL
                     HAQGAPIDAKQTSADGATPRDIALAEGHPETAALIDRLLADRR"
     gene            800..>3112
                     /locus_tag="VFL84_12285"
     CDS             800..>3112
                     /locus_tag="VFL84_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7609449.1"
                     /translation="MLHDLKNACRSLLKAPWFTCVVVLTLALGLGANTAIFGVVNKLL
                     LNPLPYADSDRLVYVGINFPGLPFGFPLPDFAARALREEARTLDRVEAFSPRSVLAYD
                     ESGARILSGMRITPSLPGLLGVAPVLGRGFSPADAEAGAPAVVMLGYEMWQRDYGGAP
                     EVVGRALTLDETSYVVVGVLPPGSAAFLGEQRPDVWFPLSLDRPPADFEFFEVLARLP
                     PGLSQETAEQEIETILNRALAASPRQIFGGDAVRARVDRPADRFGGSTREALLVLLAA
                     VGLVLLVACSNVANLLLARGVSRARELALRSALGASAWRLVRASLAECLVLALAAGAV
                     GIALGWATLRILVRLRPDSLGAPIGEVRLDATVLAFTFGISVVTALVFGLAPAAQLTS
                     RKLGDSLRHGASGAVRGGSGAGLRKALVAAQMTLSVVLLVSAGLLVRSVIYLQNVDVG
                     FDVANLFSAPLTLPRGRYEQPASREVLAEQLLANVRSMPGIAAVTQAHIAPPQYSISG
                     NDFEIRGVTVSEADASGGHAFNYVRPNFFATLGIPMLQGRTFTADELRTGNVFVISQA
                     AAQRFWPSGNAVGAEMKWGRNWGTVVGVAADVASGGMIRGKDDPQFYLPYQAAEVPSS
                     LGSPPSMLLIVRAAGDPAVAMESVRAATKALDPEIAIPYMLLTETGLAATIDGPRFNM
                     ALLTAFAAIALVLAAVGLAAVIGYEVTERTHEFGIRMALGARTENVRRLAMKHGLTPA
                     FVGVVLGVLGALAATRFAASMLYGIEPRDPL"
BASE COUNT          437 a         1062 c         1087 g          526 t
ORIGIN      
        1 tcgcgctcta caaccggcag ttcgacacgg ccgtcgcgct gatcgagcac ggcgccgacg
       61 tcgatcagtg ggactggtgg ggcaggagcc cgctctatct cgcgatcgag ctgaatcgca
      121 ttcccgacag ccggcgcggc gacttgcccg cgctcgacaa gaacacgggc ctggacgtcg
      181 ctcgcctgct gctcgagcgc ggcgcgaacg tgaacatgcg cctgaagcat cagccgccgc
      241 tgcgcaacga gcccggcgac cgcggctacg tcgacggtgg tcccgacgtg ctcgtcgtga
      301 gccccggagc gacggcgctg cacgtcgcgg cgaaagcgag cgacgatgat gcggtgaagc
      361 tgctgctcgc gcacggcgcg aacgtgcagg ccgcgaacgt cttcggcatc acacccgtca
      421 tggccgccgc gggtgtcggc cactggtacg gcgtgttccg cgagttcccg acgatcggcc
      481 gctacaagac cggcgccgac gccgtcgcga cgatgaagct gctgctcgcg gccggcgcga
      541 ggctcgacgg ccgcacgacc gagctcagca tcggctttca gcggccgcgc atcgcagggc
      601 tcacggccgc gcacggcgca gcgttccagg gctggaacga agtcatcgag ttcctgcacg
      661 cgcagggcgc gccgatcgac gcgaagcaga cgagcgccga cggcgcaacg ccgcgcgaca
      721 tcgcgctcgc cgaaggccat cccgagactg cggcgctaat cgatcgcttg ctcgccgaca
      781 ggcgctgagc gacgacgcga tgctgcacga tctcaagaac gcctgccgat cactgttgaa
      841 agctccgtgg ttcacgtgcg tcgtcgtgct cacgctcgcg cttgggctag gcgcgaacac
      901 ggccattttc ggcgtcgtca acaagctgct gctgaacccg ctgccgtacg cagactcgga
      961 ccgcctcgtt tacgtgggca tcaacttccc gggtcttccg ttcgggtttc cactacccga
     1021 tttcgccgcg cgcgcgttgc gcgaggaggc acgcacgctc gaccgcgtcg aggcgttctc
     1081 accgcgtagc gtgctcgcct acgacgagag cggtgcgcgc atcttgtcgg gtatgcgaat
     1141 cacgccgagc ctgccgggct tgctcggcgt cgcgcccgtg ctcggccgcg gcttttcgcc
     1201 cgccgatgcc gaagccggcg cgccggccgt cgtcatgctc ggctatgaaa tgtggcagcg
     1261 agattacggc ggcgcaccgg aagtagtcgg ccgcgcgctg acgctcgacg aaacgtctta
     1321 cgtcgtggtc ggcgttttgc cgccggggtc ggccgctttc ctgggcgagc agcggccgga
     1381 cgtatggttt ccgctatcgc tcgatcgccc accggcggat ttcgagttct tcgaagtgct
     1441 ggcccgcttg ccgcccgggc tctcgcaaga gacagcggag caggagatcg aaacaatcct
     1501 gaaccgcgcg ttggcggctt ctcctcgaca gatcttcgga ggcgacgccg tgcgcgcgcg
     1561 tgtcgatcgg ccggcggatc gcttcggcgg cagcacgcgc gaggcgctgc tcgtgttgct
     1621 cgcggccgtg ggtctcgtgc tgctcgtcgc atgctcgaac gtcgcgaact tgctgctcgc
     1681 gcgcggcgtg tctcgcgcgc gcgagctcgc gctgcgctcg gcgctcggcg cgagcgcgtg
     1741 gcggctcgtg cgcgcgtcgc tggccgaatg cctcgtgctc gcgctcgcgg ccggcgccgt
     1801 cggcatcgcg ctcggctggg ccacgttgcg aattctcgtt cggctgcggc ccgacagctt
     1861 gggcgcgccg atcggcgagg tgcggctcga cgcgacggtg ctcgcgttca cgttcggcat
     1921 atcggtcgtg accgcattgg tgttcggcct ggcgccggcc gcgcagctca cgtcgcgcaa
     1981 gctcggcgac tcgctgcggc atggcgcgtc gggagccgtg cgcggcggca gcggcgcggg
     2041 cttgcgcaaa gcactcgtcg cggcgcagat gacgctgtcg gtggtgctgc tcgtcagcgc
     2101 cgggctgctc gtgcgcagcg tcatctattt gcaaaacgta gacgtgggtt tcgacgtcgc
     2161 gaacctgttt tccgcgccgc tgacgctgcc gcggggccgt tacgagcagc cggcgagccg
     2221 cgaggtgctc gccgagcagc tgctcgctaa cgtgcgctcg atgcccggca tcgccgcggt
     2281 gacgcaggcg cacatcgcgc cgccgcagta ctcgatctcg ggcaacgact tcgagatccg
     2341 cggagtcacg gtcagcgaag ccgatgcgag tggcggtcac gcattcaact acgtgcgacc
     2401 taacttcttc gcgacgctcg gcatcccgat gctccagggg cgcacgttca cggcggacga
     2461 gctgcgcacc ggcaacgtgt tcgtgatcag tcaggcggcc gcgcaacgct tctggccgtc
     2521 gggcaacgct gtcggcgccg aaatgaagtg ggggcgcaat tggggaacgg tcgtcggcgt
     2581 cgccgccgat gtggcctccg gcggcatgat tcgcggcaag gacgatccgc agttctactt
     2641 gccgtatcaa gccgcagagg tgccgtcgag tctcggctcg ccgccgtcga tgctcctgat
     2701 cgtacgtgcg gccggcgatc cggccgtcgc gatggagtcg gtgcgagcag cgacgaaagc
     2761 gctcgacccc gagatcgcga ttccgtacat gctgctgacc gagactgggc tcgccgcaac
     2821 gatcgacggg ccgcgcttca acatggcgct gctgaccgca ttcgccgcga tcgcgctcgt
     2881 tcttgcggcg gtcggtctcg ccgccgtgat cggctacgag gtcacggagc gcacgcacga
     2941 gttcggcatt cgcatggcgc tcggcgcgcg gaccgagaac gtgcgccgtc tcgcgatgaa
     3001 gcatggattg actccggcgt tcgtcggtgt cgttctcggc gtgctcggcg ccctcgccgc
     3061 cacgcgattc gcggcgagca tgttgtacgg catcgagccg cgcgacccgt tg
//
LOCUS       DASSNN010000659         3939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_976067, whole genome shotgun sequence.
ACCESSION   DASSNN010000659 DASSNN010000000
VERSION     DASSNN010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976067"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VFL84_12290"
     CDS             <1..538
                     /locus_tag="VFL84_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609450.1"
                     /translation="YLGVFAEMIAVWASLALFLATGATLRAHRDELALMEGLDDAEQR
                     ELEERQSGWQKILDRAYASVRSGLPAQAYRTVKELIESEGDTLEIYQWTFNGMLAWDD
                     PRHAAMLGERFAKKLWEAGRKHDALELAQRCRKLSPSFVPPAAFTAELAAYARELGRH
                     RLADDLAELAAANRPAAP"
     gene            complement(535..1782)
                     /locus_tag="VFL84_12295"
     CDS             complement(535..1782)
                     /locus_tag="VFL84_12295"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7609451.1"
                     /translation="MTRPRIVIVGAGFGGLFAAQALARVDAEVTVVDRHNYHLFQPLL
                     YQVATAGLPPSDVAWPIRGVLRRQRNATVLLGEVVGVDANRRVVELEDRTLPFDYLVV
                     ATGSTHSYFGHDEWRPFAPGLKSIDDATEIRRRILSAFEAAEMEADAARRERLMRFVI
                     VGAGPTGVELAGTIAELARFTLASDFRRIDPRSAQIVLVEAGPRVLAAFKEPQSDYAL
                     RALLKLGVEVRLNAAVTGCDKHGVWLGAERLESETAIWAAGVAASPAGRWLGAPTDRA
                     GRVLVQPDLSTGGNVFVIGDAASVTLPDGRRVPGVAPAAKQQGRYVAKVIAARIEGRA
                     DPKPFAYRDPGNLATIGRKVAVIEFPFMTLRGFLAWWIWGIAHIYFLVGVPSPVLVSL
                     RWLWEYLTYGRGARLITGQEHAD"
     gene            2071..2730
                     /locus_tag="VFL84_12300"
     CDS             2071..2730
                     /locus_tag="VFL84_12300"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609452.1"
                     /translation="MTAAAIAQQPSQQPTPRSSQPQTQPTPPRNSQPAPDSRSTQPAP
                     APRGGDRTEQMSFERADKNADGQVSRQEANTIEGFDFSRADTNNDASLSRQEYQAAMA
                     TSTQRDGPGGRNESSGSSRSGAGGSASARSGGPQSDQSGGASRNQRDEDPGDRTGQGQ
                     QRSAQVSFDTADKDKDGKITRQEASDVPGLNFSSADTNADASLSRQEFQTAMAGAQPR
                     G"
     gene            complement(2811..3527)
                     /locus_tag="VFL84_12305"
     CDS             complement(2811..3527)
                     /locus_tag="VFL84_12305"
                     /inference="COORDINATES: protein motif:HMM:NF026184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HET7609453.1"
                     /translation="MAKLKLTVLSLRYSSWSMRPWLVLYHTGAPYETETVSLPHMARQ
                     SEDTSLADRRKLGSVRGLFPVLRVDGTPIHESLAICEYVADSFPDAHLWPAEPLQRAT
                     ARAICSEMVSGFLAMRNELSCHLFGRVPAFKPSDAARADIERVFEIWRERLEQSGGPF
                     LFGRFTIADAMYFPVLTRFRTYGIELPRSLAGYAEALESQPAVRALIAVAATAPRIPV
                     YDDYLRRFGGDPDAALHPTK"
     gene            complement(3559..>3939)
                     /locus_tag="VFL84_12310"
     CDS             complement(3559..>3939)
                     /locus_tag="VFL84_12310"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7609454.1"
                     /translation="DYLSRHRLAPGAREFLTSAHRANLPVWCLSNDVGRWSRRLREAL
                     GLQQLLAGAVISSDAKVRKPDRGIYEHLLVATGCRPAELLFIDDRAKNVAGAAAVGIP
                     AVEFTGSDVYGRLSRKLFARQAEV"
BASE COUNT          708 a         1329 c         1342 g          560 t
ORIGIN      
        1 gtatctcggg gtcttcgccg agatgatcgc cgtgtgggcg agcctcgcac tgttcctcgc
       61 gaccggagcg acattgcgcg cgcatcgcga cgagctcgcg ctaatggagg gcctcgacga
      121 cgcggagcag cgcgagctcg aggagcgcca gtcgggctgg cagaagatcc tcgaccgcgc
      181 gtacgcctcg gtgcgcagcg gtttgccggc gcaggcctat cgcaccgtca aggagctcat
      241 cgaaagcgaa ggcgacacgc tcgagatcta tcagtggacg ttcaacggca tgctcgcctg
      301 ggacgacccg cggcacgcgg ccatgctcgg cgagcggttc gcgaagaagc tctgggaggc
      361 cgggcgcaag cacgacgcgc tcgagctcgc gcagcgctgc cgcaagttga gcccgagctt
      421 cgtgccgccc gcggcgttca cggccgagct cgcggcctac gcgcgcgagc tcggccggca
      481 tcgtctcgcc gacgacctcg ccgagctcgc ggcggcgaac cggccggccg cgccttagtc
      541 ggcgtgctct tggcccgtga tgagccgcgc gccgcggccg tacgtcaagt attcccagag
      601 ccagcgaagc gatacgagca cggggctcgg cacgccgacg aggaagtaga tgtgcgcgat
      661 gccccagatc caccacgcga gaaagccgcg tagcgtcatg aacggaaact cgatgacggc
      721 gaccttgcgc ccgatcgttg cgaggttgcc ggggtcgcga taggcgaaag gcttggggtc
      781 ggcgcgccct tcgatgcgcg ccgcgatgac cttcgcgacg tatcgtcctt gctgcttcgc
      841 ggccggcgcg acgcccggca cgcggcggcc gtccggcaga gtcacgctcg ccgcgtcgcc
      901 gatgacgaac acgttgccgc ccgtcgacag atcgggctgc acgagcacgc ggccggcgcg
      961 atccgtcggc gcgccgagcc agcgtccggc cggcgacgcc gcgacgcccg cggcccagat
     1021 cgcggtctcg ctctcgagcc gctcggcgcc gagccatacg ccgtgcttgt cgcagcccgt
     1081 gaccgcagcg ttcaacctga cttcgacgcc gagcttcaag agcgcgcgca gcgcgtagtc
     1141 cgactgcggc tctttgaacg ccgcgagcac gcgcggcccc gcttcgacga gcacgatctg
     1201 cgcgctgcgc gggtcgatgc ggcggaaatc ggaggccaac gtgaatcgcg cgagctcggc
     1261 gatcgtgccc gcgagctcga cgcccgtcgg tccggcgccg acgatgacga agcgcatcag
     1321 ccgctcgcgg cgagccgcgt cggcctccat ctcggcggcc tcgaacgcgc tcaggattcg
     1381 gcgtcgaatc tccgtcgcgt cgtcgatcga tttcagaccc ggcgcaaacg gtctccattc
     1441 gtcatggccg aaatacgaat gcgtcgagcc cgtcgcgacg acgaggtaat cgaacggcag
     1501 cgtgcgatcc tcgagctcga cgacgcgccg gttcgcgtcc acgccgacga cttcgccgag
     1561 cagcaccgtc gcattgcgct gccggcgcaa gacgccgcga atcggccacg cgacgtccga
     1621 tggcggcagg cccgccgtcg cgacttgata caacagcggt tggaacaagt ggtagttgtg
     1681 ccggtcgacg acggtgactt cggcgtcgac gcgtgcgagc gcttgcgccg cgaacaatcc
     1741 gccgaagccc gcgccgacga tcacgattcg agggcgcgtc attcgatgag gagcggccgc
     1801 ggaggtgagt aggcggaaat atatgacagc gactggacgc gctcatagtc cctgtgcgca
     1861 tggcggcgat tgtccgatct ggaattgcat tcccaagctg cgaaagcgcg cgtgctcgca
     1921 gcctcgatgc gtgcagaaag tgggtggcat gtgtcttgca gtccatgcgc cgcttgccgc
     1981 gcaccacgcg gtgggggctt ctatcaatca cgaggagatg cagtcatgag gatgaagaga
     2041 ttagcttttg caacattggt ggcgatgggc atgaccgcag ctgcgatcgc ccagcagccc
     2101 agtcagcaac cgactccgcg gtcgtcgcaa ccacagacgc agccgacacc gccgcgcaac
     2161 agccagccgg caccggactc gcgcagcacg cagcctgcgc cggctccgcg cggcggcgat
     2221 cgcaccgagc agatgagctt cgagcgtgcc gacaagaacg ccgacggtca ggtcagccgg
     2281 caagaggcga acaccatcga agggttcgat ttctcgcgcg ccgacacgaa caacgacgcg
     2341 tcgttgagcc gtcaggagta tcaagcggcg atggcgacgt cgacgcagcg cgacggtccg
     2401 ggcgggcgca acgagtcgag cggcagctcg cgctcaggag ccggcggcag tgcaagcgcg
     2461 cgcagcggcg gcccgcagag cgatcaatcg ggcggcgcga gccgcaatca gcgcgacgag
     2521 gatcccggcg accgcaccgg ccagggtcag cagcgcagcg cccaggtaag cttcgacacg
     2581 gccgacaagg acaaggacgg caagataacg cggcaagaag cgagcgacgt tccgggcctc
     2641 aacttctcgt ccgccgacac gaatgcggac gcatcgttga gccgtcagga gtttcaaacc
     2701 gcaatggcgg gcgcccagcc gcgaggctag gcatcgtacg gcgcacgccg gccgcaaggc
     2761 cgcacgcgcc taccctgtcc gtcgagatga ggccgcgcga aagcgcggcc tcattttgtg
     2821 gggtgcaacg cggcgtccgg atcgccgccg aaacggcgca gataatcgtc gtaaacggga
     2881 atgcgcggcg ccgtggcggc gacggcgatc agcgcgcgca cggcgggctg cgactcgagc
     2941 gcttcagcgt aaccggcgag cgagcgcggc agctcgatgc catacgttcg aaagcgcgtc
     3001 agcacgggaa agtacatcgc gtcggcgatc gtgaagcggc cgaacaggaa cggaccgccg
     3061 gactgctcga ggcgttctct ccagatctcg aagacgcgct cgatgtccgc gcgcgccgcg
     3121 tcgctcggct tgaacgccgg gacgcggccg aatagatggc acgagagctc gttgcgcatc
     3181 gccaagaagc cgctgaccat ttccgagcag atcgcgcgcg ccgttgcgcg ctgcagcggc
     3241 tcggcgggcc acaggtgcgc atccggaaac gagtcggcga catactcgca aatcgcgagc
     3301 gactcgtgga tcggcgtgcc gtccacgcgc agaacgggga acaggccgcg gacgctgccg
     3361 agcttgcggc gatccgcgag agaggtgtcc tcgctctgcc gcgccatgtg cggcagcgaa
     3421 accgtttcgg tctcgtacgg tgcccccgtg tgatagagca cgagccacgg gcgcatcgac
     3481 cacgatgagt aacgcaaact caaaacggtg agcttcagtt tcgccatgcg tgcacgatgc
     3541 cgccggtatc ggtcgtagtc aaacttcggc ttgccgcgcg aaaagcttcc tgctcaggcg
     3601 accgtaaaca tccgatcccg tgaactctac cgccgggatg ccgaccgccg cggcgcccgc
     3661 gacgttcttc gctcgatcgt ctatgaacaa cagctctgcc ggccggcacc cggtcgcgac
     3721 gagcaagtgc tcgtagattc cgcgatccgg ctttcggacc ttcgcgtcgc tgctgatgac
     3781 ggctcccgca agcagttgct ggaggcccaa cgcctcgcgc aaccgcctcg accatcggcc
     3841 cacgtcgttc gacagacacc aaaccgggag gttggctcgg tgcgcgctcg tcaagaactc
     3901 gcgcgcgccg ggcgccaaac ggtgtcttga caggtagtc
//
LOCUS       DASSNN010000660         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_976527, whole genome shotgun sequence.
ACCESSION   DASSNN010000660 DASSNN010000000
VERSION     DASSNN010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976527"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..482)
                     /locus_tag="VFL84_12315"
     CDS             complement(90..482)
                     /locus_tag="VFL84_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7609455.1"
                     /translation="MAAKLNHTIVWCRDQVASANFLTEILGLPPPRRFMGFLVVDLAN
                     DVSVDYMAAESEPIALQHLAFLVSEQEFDGALARVRERTIEIWADPAKKQPGRINRHF
                     GGRGVYFDDPNGHLFEIITTPYGDPATL"
     gene            complement(489..1199)
                     /locus_tag="VFL84_12320"
     CDS             complement(489..1199)
                     /locus_tag="VFL84_12320"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HET7609456.1"
                     /translation="MWSPLRASFAAAALAATLIGPATAGAETVAMIGTGNVGAALGRR
                     FAEHGHAVVYGSRDPSAADVRELVAVTGHGAVARSQAQAAASADIVVLAVPWTAVEDV
                     VRALPDLTGKIVVDPTNPRVTAGDGFADYPIEDSNAERIARLAPGATVVKAFSTLGFE
                     TMLDPTVAGGPVTVPIVGDDRAAKERVAVLAREIGLEAVDVGPLRHARIIEGLHYLRA
                     NAYGGRINYHLPRDHARD"
     gene            complement(1225..2424)
                     /locus_tag="VFL84_12325"
     CDS             complement(1225..2424)
                     /locus_tag="VFL84_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7609457.1"
                     /translation="MRQRDFLKLIAQSIVAHRLRSVLTALGIGIGVTAVVLLTSIGEG
                     LNTYMVEMFTQFGTTTVQIQPGKTSTFGFTPGVLNSTRPLSLDDAAALERAPHVLASV
                     PVVAGSASVEGNGRERSVTVYAVGPEFDRAFKFDVVSGTFLPHDELERARNVAVLGAK
                     VHSELYGGANALGERIRVGGQRYRVVGIMESKGDMVGIDLDDTVYIPAARGLELFNRQ
                     GLLEIDVLYAEDAPVDEVVAGIKRILAARHGAEDFTVVTQQQMLDVLGSIVEVLTLGV
                     AALGGISLLVGGVGIFTIMTIAVRERTQEIGLLRAIGARRSAIAQLFLGEAMLLSGAG
                     GAGGLALGMGIVLLVQWTLPAMPATISPLYVVLSLALAVLIGAIAGVLPARSAARLEP
                     LDALRAE"
     gene            complement(2430..>3548)
                     /locus_tag="VFL84_12330"
     CDS             complement(2430..>3548)
                     /locus_tag="VFL84_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7609458.1"
                     /translation="VAAVVTLTAVGEGARRYVTGQFSSLGTELLIVLPGRAETSGGGI
                     QGLLVGETARELTLDDTLALTRSPRIKQVTPLVLGAGTASSGARERDITVLGTGAAML
                     AIQHWLMSSGRFLPEMPLDTAQPVCVLGHTVARELFAGRPAVGDWVRIGDTRCRVLGV
                     LAEQGMAGGFNVDETVIMPVASAQQIFNTSAVFRILVEAISLDAVPAARRDIIEIVKA
                     RHAGEEDITVVTQDALVSTFDTIFNMITAGLAAIAGISLVVAGVLIMNVMLVAVSQRT
                     AEIGLLKAVGARNRQVLALFLTEAACLSLLGALVGLAFGTGGIWLMKALFPVLDFAAP
                     AWASASAVAVAVLSGLVFGIMPARRAAALDPVDALMKR"
BASE COUNT          579 a         1214 c         1237 g          518 t
ORIGIN      
        1 tgcgcgatca agcgcagacg cagatgagca aaaggatttt tcgcgcgtcg tcgccacgcg
       61 gcgacaaagc gcgtcgcggc tgctccaaat tacagagtcg cgggatcgcc gtacggcgtc
      121 gtgatgatct cgaacaggtg cccgttggga tcgtcgaagt agactccgcg gccgccgaag
      181 tgacggttga tgcggccggg ctgtttcttc gcgggatcgg cccagatctc aatcgtgcgc
      241 tcgcggacgc gcgcgagcgc gccgtcgaac tcttgctcgc tgacgagaaa cgcgaggtgc
      301 tgcagcgcaa tcggctcgga ctcggccgcc atatagtcga ccgagacgtc gttggcgaga
      361 tcgacgacga gaaaacccat gaaacgccgc ggcggcggta ggccgagaat ctcggtcagg
      421 aaattcgcgg acgcaacctg atcgcggcac caaacgatcg tgtggttgag cttggcggcc
      481 atgctccctc agtcgcgcgc gtggtcgcgc ggcaaatggt agttgatgcg cccgccgtaa
      541 gcgttggcgc gcaagtagtg caggccttcg atgatgcgcg cgtggcgcaa ggggccgacg
      601 tcgacggcct cgagcccgat ctcgcgggcg agcacggcga cgcgttcctt cgccgcgcga
      661 tcgtcgccga cgatcggcac tgtgacgggc cctccggcga ccgtggggtc gagcatcgtc
      721 tcgaagccga gcgtgctgaa cgctttgacg accgtggcgc ccggagcgag tcgcgcgatg
      781 cgctcggcgt tcgagtcctc gatcggatag tcggcgaagc cgtcgccggc cgtgacgcgc
      841 gggttcgtcg ggtcgacgac gatcttgccc gttagatcgg gcagcgcgcg gacgacgtcc
      901 tcgacggcgg tccacggcac ggcgagcacg acgatgtcgg cgctcgccgc cgcttgtgct
      961 tggcttcgcg cgaccgcgcc gtggccggtg acggcgacga gctcgcgcac gtcggccgcg
     1021 gacggatcgc gcgagccgta gacgaccgca tggccgtgct cggcgaagcg ccgcccgagc
     1081 gccgcgccga cgttgccggt gccgatcatc gcgacggttt ccgcgccggc agtcgcgggg
     1141 ccgatcagcg tcgcggcgag cgccgcggct gcgaacgatg ctcgaagtgg actccacatc
     1201 gcgagactct cctttgatcg ccagtcactc ggcgcgtaaa gcgtcgagcg gctcgagccg
     1261 cgccgcgctg cgcgccggaa gcacgcccgc gatcgcgccg atcagcaccg cgagcgccaa
     1321 cgacagcacc acgtagagcg gcgagatcgt cgcgggcatc gccggcagcg tccactgcac
     1381 gagcagcacg atgcccatcc cgagcgcgag cccgccggcg ccgcccgcgc ccgaaagcag
     1441 catcgcctcg ccgaggaaga gctgcgctat tgccgagcgc cgcgcgccga tcgcgcgcag
     1501 caagccgatc tcctgcgtcc gctcgcgcac cgcgatcgtc atgatcgtaa agattccgac
     1561 gccgcccacg agcaacgaaa tgccgcccag cgccgccacg ccgagcgtca gcacttcgac
     1621 gatcgacccg agcacgtcca gcatctgctg ttgcgtgacg accgtgaagt cctcggcgcc
     1681 gtggcgagcc gcaagaattc tcttgatgcc ggccacgacc tcgtcgacgg gcgcgtcctc
     1741 ggcatagagc acgtcgatct cgagcagccc ttgccggttg aacagctcga ggccgcgcgc
     1801 agcggggatg tagacggtgt cgtcgagatc gatgccgacc atgtcgccct tcgactccat
     1861 gatgccgacg acacgatagc gctggcctcc gacgcggatc cgctcgccga gcgcgttggc
     1921 gccgccgtac agctccgaat ggaccttagc gccgagcacg gccacgttgc gcgcgcgctc
     1981 gagctcgtcg tgcggcaaga acgtgccgct gacgacgtcg aacttgaacg ccctgtcgaa
     2041 ctcgggaccg acggcgtaaa ccgtcacgct gcgctctcgg ccattgcctt cgaccgatgc
     2101 gctgccggcg acgaccggaa cggaggcgag cacgtgcggc gcgcgctcga gcgccgcggc
     2161 atcgtcgagc gagagcggcc gcgtgctgtt caacacgccg ggcgtgaagc cgaacgtgct
     2221 cgtcttgccg ggctgtattt gcacggtcgt ggtgccgaac tgcgtgaaca tctcgaccat
     2281 gtacgtgttc aggccctcgc cgatcgacgt cagcagcacg acggccgtga cgccgatgcc
     2341 gatgccgagc gccgtgagca cgctccgcag cctgtgcgcg acgatggact gggcgatcag
     2401 cttgaggaag tctcgctgcc gcatcgcgct caccgcttca tgagcgcgtc gaccgggtcg
     2461 agcgccgcgg cacgccgcgc gggcatgatg ccgaacacga gcccgcttag cacggcgacg
     2521 gcgacggccg aggccgacgc ccacgcgggg gccgcgaaat cgagcaccgg gaacagggct
     2581 ttcatgagcc agatgccgcc cgtgccgaac gcgagtccga ccaaggcgcc gagcagcgac
     2641 aagcacgccg cttccgtgag gaacagcgcg aggacttgcc ggttccgcgc gccgacggcc
     2701 ttcaagaggc cgatctcggc cgtgcgctgg ctcacggcga cgagcatgac gttcatgatc
     2761 agcacgccgg cgacgacgag acttatgcct gcgatcgcgg cgaggccggc cgtgatcatg
     2821 ttgaagatcg tgtcgaacgt cgataccagc gcgtcctgtg tgacaaccgt gatgtcttcc
     2881 tcgccggcgt ggcgcgcctt gacgatctct atgatgtcgc ggcgcgcggc cggcacggcg
     2941 tcgaggctga tcgcctcgac gaggatgcgg aacacggccg aggtgttgaa gatctgctgc
     3001 gcgctcgcga cgggcatgat cacggtctcg tcgacgttga agccgccggc catgccctgc
     3061 tcggcgagca cgccgagcac gcggcagcgc gtgtcgccga tgcgcaccca gtcgcctacg
     3121 gccggccgcc cggcgaagag ctcgcgcgcg acggtgtggc cgagcacgca gaccggctgc
     3181 gccgtgtcga gcggcatctc gggcaggaac cggccgctgc tcatgagcca atgttggatc
     3241 gcgagcatcg cggcgccggt cccgagcacc gtgatgtcgc gttcgcgcgc gccggaagaa
     3301 gccgtgcctg cgccgagcac gagcggcgtc acttgcttga tgcgcgggct gcgcgtcagc
     3361 gcgagcgtat cgtcgagggt gagctcgcgc gccgtctcgc cgacgagcag gccttggatg
     3421 ccgccgcccg acgtctcggc gcggcccggc agcacgatca ggagctcggt gccgagcgac
     3481 gagaattgac ccgtgacgta gcggcgcgct ccctccccga cggccgtgag tgtgacgacg
     3541 gccgcgac
//
LOCUS       DASSNN010000661         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1861100, whole genome shotgun sequence.
ACCESSION   DASSNN010000661 DASSNN010000000
VERSION     DASSNN010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861100"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1508)
                     /locus_tag="VFL84_12335"
     CDS             complement(192..1508)
                     /locus_tag="VFL84_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HET7609459.1"
                     /translation="MTRRDDRLANWILLGLVVGAAAGAATLTIGALAPAVLTGARAVA
                     TYALDPLGQVFLRLLFFVVMPLVFASLAAGVVQLGRLSRLGPLAVRTFALFFFNMAIA
                     VALGLIMMNVVEPGNRLDDATQARLVEQFGGAAQQHIERSAQSPALTPSTIVDMFMPR
                     NLLGSFVGNDRNALGEVLPLIVFAILVGAAGAALAPDKRARLQAGLDLVTELMTSIVH
                     FALRLAPYAVAGMIFSVVVKIGFDILVALGVFVVGCAACMLIHLFGTMSLWLKLWTRH
                     RPREFFRQIKSVLVTAFSTSSSAATLPAALDAAQNQLAVRPSVAGFVLPLGTTMNMSG
                     TALYEGCVVLFVAQVFGFDLGFGEQITLLVLAVFSAVAVAAIPGGSLPLIAGLLITFG
                     IPPEGIGIVLGVDRLLDMTRTMVNVGSDVVTTLVVDARTPATEADA"
     gene            1592..>2509
                     /locus_tag="VFL84_12340"
     CDS             1592..>2509
                     /locus_tag="VFL84_12340"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609460.1"
                     /translation="MSSRTFALLLGLLTAPAFAHHSFAPHFDIDKPVDISGTVTEYEA
                     RNPHSYLHIAAVDENGKTQEYVCESHGVTQLSRNGITPAMLKPGTELRVDGSQSRHSP
                     HMCFFNNVYFPDGRALNVNGPRGAPPPRALPARSDIFGTWMLAPAAPDARRANTSRPQ
                     PMMNFLTPAGAAAVAAYDPFKDDPTFRCDPVAIRRVWGAPGTPLSITREGNDVVLRHE
                     WMDVVRTVHLAQREHPANGPRTSLGHSIGRFDGGTLVIETANYSAGVLNQYVEEPGKP
                     TRGLLHSAALTSVERLSFDAARQRLVVEVE"
BASE COUNT          461 a          863 c          827 g          358 t
ORIGIN      
        1 aactcgcgcc ggtcgatcct gccgtggcag tactcgtcgt aaagatcgta gacgcgttga
       61 tcgatcgaga gcggtgctgc gcttctcagg tcttgcggtt gtgcggccat ggttcctccg
      121 aattgttgga agtcgaaagc cggtcgtggc gaacgaaatt taccgcgccg gctcaaccct
      181 gtcgattcgg ctcacgcgtc cgcttcggtc gccggcgtgc gcgcgtccac gacgagcgtg
      241 gtcacgacgt ccgagccgac gttgaccatc gtgcgcgtca tgtcgagcag ccgatcgacg
      301 ccgagcacga tgccgatgcc ttcgggcgga attccgaacg tgatcaagag acccgcgatg
      361 agcggcaacg atccgcccgg aatggccgcg acggcgacgg cgctgaacac ggcgagcacg
      421 agcagcgtga tttgctcgcc gaagccgaga tcgaagccga agacctgcgc cacgaacagc
      481 acgacgcagc cctcgtagag agccgtgccg ctcatgttca tcgtcgtgcc gagcggcaac
      541 acgaatcccg cgacggatgg gcgcacggcg agctgattct gcgccgcgtc gagcgcggcc
      601 ggcagcgtcg ccgcgctcga gctcgtcgaa aacgcggtca cgagaaccga cttgatctgg
      661 cgaaaaaatt ccctcggccg gtgccgtgtc cagagcttga gccagagcga catcgtgccg
      721 aacagatgaa tcagcatgca ggcggcgcag ccgacgacga atacgccgag cgcgacgagg
      781 atgtcgaaac cgatcttgac gacgacgctg aaaatcatgc ccgccacggc gtacggcgcg
      841 aggcgcagcg cgaagtgcac gatcgaagtc atgagctcgg tgacgaggtc gagcccggcc
      901 tgaagccgcg cacgcttgtc tggcgcgagc gccgcgccgg cggccccgac caagatcgcg
      961 aacacgatca acggcaagac ctcgccgagc gcgttgcggt cgttgccgac gaacgagccg
     1021 aggaggttgc gcggcatgaa catgtcgacg atcgtgctcg gcgtcaaagc cggcgactgc
     1081 gcactgcgct cgatgtgttg ctgcgcggca ccgccgaact gctcgacgag ccgcgcctga
     1141 gtcgcgtcgt cgagccgatt gccgggctcg accacgttca tcatgatgag gccgagcgcg
     1201 acggcgatgg ccatgttgaa gaagaacagc gcgaacgtgc ggaccgcgag cggccccaaa
     1261 cggctcaagc ggccgagctg aacgacgccg gccgcgagcg acgcgaacac gagcggcatg
     1321 acgacgaaga agagcagccg caggaacacc tggccgagcg gatcgagcgc gtacgtcgcg
     1381 acggctcgcg cgcccgtcaa cacggccggc gcgagcgcgc ctatggtcag cgtcgcggcg
     1441 cccgccgcgg cgccgacgac caacccgagc agaatccagt tcgcgaggcg gtcgtcacgg
     1501 cgcgtcatcg cggcacgcta gcaaaatccg ctgcgcggcg cgatgggccc cgaatatact
     1561 catagccacc ttcgaagagc attcgccgcc tatgagctcg agaacttttg ccttgctgct
     1621 cggcttgctc accgctccgg ccttcgcgca ccacagcttc gcgccgcact tcgacatcga
     1681 caagcccgtc gacatctcgg gaacagtgac cgagtacgaa gcgcgcaatc cgcacagcta
     1741 cctgcacatc gccgccgtcg acgagaacgg caagactcaa gagtacgttt gcgaatcgca
     1801 cggcgtcacg cagctctcgc ggaacggcat cacgcccgcg atgctgaagc ccggcacgga
     1861 gctgcgcgtc gacggctcgc agtcccggca tagcccgcac atgtgcttct tcaacaacgt
     1921 ctatttcccc gacggccgcg cgctcaacgt caatggcccg cgcggcgcgc cgccgccacg
     1981 cgcgttgccg gcgcgcagcg acatcttcgg cacctggatg ctggcgccgg ccgctcccga
     2041 cgcgcgccgc gccaacacga gccggccgca gccgatgatg aatttcctga cgccggccgg
     2101 cgccgcggcc gtcgcggcgt acgatccgtt caaggacgac ccgacgttcc gctgcgaccc
     2161 cgtcgcgatt cgccgtgtct ggggcgcgcc cggcacgccg ctatcgatca cgcgcgaggg
     2221 caacgacgtc gtcctgcgcc acgagtggat ggacgtcgtg cgcaccgtgc acctcgccca
     2281 gcgcgagcac ccggcgaacg ggccgcgcac gtcgctgggt cattcgatcg gccgcttcga
     2341 cggcggcacg ctcgtcatcg agacggccaa ctattcggcg ggcgtgctga atcagtacgt
     2401 cgaggagccc ggcaagccga cgcgcggcct gctgcactcg gccgccttga cgtcggtcga
     2461 gcgcttgtcg ttcgacgcgg cgcggcagcg gctcgtcgtc gaggtcgag
//
LOCUS       DASSNN010000662         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_976677, whole genome shotgun sequence.
ACCESSION   DASSNN010000662 DASSNN010000000
VERSION     DASSNN010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976677"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /gene="ccmE"
                     /locus_tag="VFL84_12345"
     CDS             complement(<1..392)
                     /gene="ccmE"
                     /locus_tag="VFL84_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET7609461.1"
                     /translation="MKSRTQRKLFIGLVLAGITLAGFLAYRALQENLLYFFSPSQVAA
                     GEAPNRTFRLGGMVVEGSLKREIGTLNVTFLVTDNEHSVPVKYNRVLPDLFREGAGAV
                     VVGRMNDKGEFIGDEVIAKHDENYMPPEV"
     gene            complement(389..562)
                     /gene="ccmD"
                     /locus_tag="VFL84_12350"
     CDS             complement(389..562)
                     /gene="ccmD"
                     /locus_tag="VFL84_12350"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HET7609462.1"
                     /translation="MNGHGAYVWSAYGITLAVLILNVWLARAKHRSALQRARELAAQA
                     EVPRIKPTVRRLQ"
     gene            complement(577..1329)
                     /gene="ccmC"
                     /locus_tag="VFL84_12355"
     CDS             complement(577..1329)
                     /gene="ccmC"
                     /locus_tag="VFL84_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HET7609463.1"
                     /translation="MKLPRWFHQLGSPPYVYRLAERLCPWLGGIGVALLAVGAYWGLV
                     IAPPDYQQGDSYRIIYIHPQTAYVGMMAYVVMAVASAIGYIWRLKLAHAVAVSAAPIG
                     AWFTFCALFTGAVWGRPTWGTYWQWGDPRIMFEALLLFLFLGYLALRGAFDDRDKADR
                     VTAILAVVGVVNIPIVHFSVEWWNSIHQGPTILRAGGPAMTNDMLVPFLIMVLGFTLT
                     FYWLLLRRLQAEIVEREGDARWIRELVGQGAP"
     gene            complement(1340..2005)
                     /gene="ccmB"
                     /locus_tag="VFL84_12360"
     CDS             complement(1340..2005)
                     /gene="ccmB"
                     /locus_tag="VFL84_12360"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HET7609464.1"
                     /translation="MSVFQAVLLRDLRVSFRRWSELAHPLIFFVIVVTLFPLALSPTA
                     SQLREVGVGVLWVATLLSSLLAVDGLFRGDADDGSLEQLLLSPAPMTLTVLAKVTAHA
                     LVTLVPLICLVPLSAYTFNIPLAALPTLAAALLLAAPTLSVLVALGAALTVSLRRGGS
                     IVGLLVLPLTAPLLIFGTRATDLSVNGEPTAAPLLLLGALAALAVSLGPLAIAAAVKV
                     GLE"
     gene            complement(2002..>2341)
                     /locus_tag="VFL84_12365"
     CDS             complement(2002..>2341)
                     /locus_tag="VFL84_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7609465.1"
                     /translation="TVVENLAFHAAIWDRKQAFEQVLEELKLEQVAHMRARYLSAGQR
                     RRVALAMLKLQGAKLWLLDEPTTNLDSEGRAVLVDWTRRHVMAGGLAVIATHQPDEFA
                     MSGALMIDLA"
BASE COUNT          466 a          794 c          732 g          349 t
ORIGIN      
        1 acctcgggcg gcatgtagtt ttcgtcgtgc ttggcgatga cttcgtcgcc gatgaactcg
       61 cctttgtcgt tcatgcggcc cacgacgacc gcgcccgctc cttcgcggaa caggtccggc
      121 agaacgcggt tgtatttgac cggcaccgaa tgctcgttgt ccgtcacgag gaacgtcacg
      181 ttgagcgtgc cgatctcgcg cttcaggctg ccctcgacga ccatgccgcc gagccggaag
      241 gtgcgattcg gagcttcgcc ggccgcgacc tgcgacgggc tgaagaaata caaaaggttt
      301 tcctgcagcg cgcgatacgc caggaacccg gcgagcgtga tgccggcgag caccagccct
      361 atgaacagct ttcgttgcgt tctcgacttc attgcagccg tcgcaccgtg ggtttaatgc
      421 gcggaacttc agcctgcgcg gcgagctcgc gggcacgctg caatgcggac cgatgctttg
      481 cgcgcgcgag ccacacgttg aggatcaaca cggccagtgt aatgccgtac gcggaccaaa
      541 cgtaagcgcc gtggccgttc attaagaaga attcgctcac ggcgcgccct gccccactag
      601 ctcgcgaatc cagcgcgcgt cgccctcgcg ctcgacgatc tcggcctgca atcgccgcag
      661 caacaaccag tagaacgtca gcgtgaaccc gagaaccatg atcaggaacg gcacgagcat
      721 atcgttcgtc atcgccggcc cgccggcgcg caagatggtc ggaccctgat gaatcgagtt
      781 ccaccattcg accgaaaaat gaacgatcgg aatgttgacg accccgacga ccgctaggat
      841 cgcggtgacg cggtccgctt tgtctcggtc gtcgaaggcg ccgcgcagcg cgagatagcc
      901 caggaacagg aacagcagca gggcctcgaa catgatgcgt ggatcgcccc actgccagta
      961 agttccccac gtgggacgcc cccacacggc gcccgtgaac aaggcgcaga acgtgaacca
     1021 ggccccgatc ggcgcggcgc tgaccgccac ggcgtgagcg agcttcaacc gccagatgta
     1081 gccgatcgcc gacgcgaccg ccatgacgac gtaggccatc attccgacgt acgccgtttg
     1141 cgggtggata tagatgattc gatacgaatc gccctgctga tagtccggcg gagcgatcac
     1201 gagaccccag tacgcgccga cggcgagcag cgcaacgccg attccgccca accacgggca
     1261 gagacgctcg gccaaacggt agacgtaggg tggcgagccg agctggtgga accaacgtgg
     1321 cagcttcatt cgctgatcct cactcgagcc ccactttcac cgccgccgcg atcgcgagcg
     1381 gccccagcga aaccgcgagc gccgcgagcg cgccgagcag cagcagcggc gccgccgtcg
     1441 gctcgccgtt gacgctcaag tccgtggcgc gcgttccgaa gatcagtagc ggcgccgtca
     1501 gaggcagcac gagcagcccg acgatcgagc cgccgcggcg caagctcacc gtcagcgccg
     1561 ctccgagcgc gacgagcacg ctcaacgtcg gcgcggcgag caacagcgcc gccgcgagcg
     1621 tcggcaacgc cgccaacgga atgttgaacg tgtacgccga cagcggcacg aggcagatca
     1681 gcggcacgag cgtcacgagc gcgtgtgccg tcactttcgc gagcaccgtg agcgtcatcg
     1741 gcgcggggct cagcaagagt tgctcgaggc tgccgtcgtc tgcgtcgccg cggaacaatc
     1801 cgtcgaccgc gagcagcgac gacagcagcg tcgcgaccca caacacgccg actccgacct
     1861 cgcgcagctg cgacgccgtc ggggacaacg ccagcggaaa cagcgtcacg acgatgacga
     1921 agaagatcag cggatgcgcg agctcgctcc agcgccggaa cgagacgcgc aaatcgcgca
     1981 acaacactgc ctggaatacg ctcatgccag atcgatcatc aatgccccgc tcatcgcgaa
     2041 ctcgtcgggc tgatgcgtcg cgatgacggc gaggcccccc gccatcacat gacggcgcgt
     2101 ccagtcgacg aggactgcgc gtccttcgct gtcgaggttc gtcgtaggct cgtcgagcaa
     2161 ccagagcttg gctccctgaa gcttcagcat cgcgagcgcc acgcgccggc gttgtcccgc
     2221 cgacaagtag cgcgcacgca tatgagcgac ctgctcgagc ttcagttcct cgagaacctg
     2281 ctcgaacgcc tgcttgcggt cccagattgc cgcgtggaaa gcgaggttct cgacgacggt
     2341 g
//
LOCUS       DASSNN010000663         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_617696, whole genome shotgun sequence.
ACCESSION   DASSNN010000663 DASSNN010000000
VERSION     DASSNN010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617696"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VFL84_12370"
     CDS             complement(<1..880)
                     /locus_tag="VFL84_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170537.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET7609466.1"
                     /translation="MSAAQHTPVMQQYHAIKARFPDTLLFYRMGDFYELFYDDARRTA
                     KLIDIALTRRGQSGGEPIPMAGVPVHSVETYLARLVRLGESVAICEQMGDPATSKGPV
                     ARDVVRIVTPGTLTDAALLDDRETRLLAAVTFTPRIGLAWLDLAGGRFSLTELESLES
                     LHAEVDRLKPAELLLAEDLDAPPWLAGLAGARRRPPWHFDPGAGKRLLCEQLGTLDLS
                     GFDAEDAHAGVGAAGCLLQYAKDTQRAALPHLRALTRERREHAVIMDAQTRRNLEIET
                     SLGVRAEYTLAGLMDRC"
     gene            1022..1708
                     /locus_tag="VFL84_12375"
     CDS             1022..1708
                     /locus_tag="VFL84_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7609467.1"
                     /translation="MPRSARVVVPAHAYHVFQRGHNQQACFAETGDYERYLANLAELK
                     EELGVKVYAWCLMTNHVHLLLEPSTATSLGLLMKRLSGRQTRYHNRLEQRSGTLWEGR
                     YKSSLVQSEAYLLACCRYIELNPVRACIVAAPEDYRWSSCRARLGYARSTILDLDPCY
                     QELAADESVRRERYREFLQSAIPEGEWALIRTAVRRGQLTGNEAFRQQVAATLHRRIE
                     NRGPGRPARK"
     gene            complement(1783..>2778)
                     /locus_tag="VFL84_12380"
     CDS             complement(1783..>2778)
                     /locus_tag="VFL84_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HET7609468.1"
                     /translation="FSDPNMPPVNDLPNPYSDRQVMPLPDGRTWGSTAGVDAAKGRDD
                     VWAIDRCGSNTCVGSHANPLLHYDAAGRLIGQMGADLFAFPHGIHVDDDGNVWVTDPL
                     PPDGRGAGGNVGQQVTKLSPSGEVLLQLGTRKVAGDPPDHFSSPSDVVVGRNGDIFVA
                     DGHGEGTSERIIKFDKDGKFIKSWGKAGNGPCGAGEFSSLHAIAIDSRGRLFIGDRDN
                     NRIQIYDQDGNYLDCWYQFSRPSGIYIDADDNLFVADSESRDGVNEGRDLAHPGWQRG
                     IRIGSARDGSVRYFIPDPDPQGGSSAAEGVAAINGGAVVYGAEVGPRSFVRYTRQ"
BASE COUNT          462 a          877 c          945 g          494 t
ORIGIN      
        1 cgcagcggtc catcaagccc gccagcgtgt actcggcgcg cacgccgagg ctcgtctcga
       61 tctcgagatt gcggcgcgtc tgcgcatcca tgatcacggc atgctcgcgc cgctcgcgcg
      121 tcagcgcgcg cagatgcggc agcgccgcgc gctgcgtgtc cttcgcgtac tgcagcagac
      181 agcccgccgc gccgacgccg gcgtgcgcat cttcggcgtc gaagccggag agatcgagag
      241 tgccgagctg ctcgcagagc agccgtttgc cggcgccggg atcgaagtgc cacggcggcc
      301 ggcgccgcgc gcccgccaga cctgcgagcc acggcggcgc gtcgagatcc tcggcgagca
      361 agagctccgc cggcttcagg cggtcgacct cggcgtgcag cgattcgagg ctctcgagct
      421 cggtgaggct gaaccggccg cccgcgaggt cgagccacgc gagcccgatg cgcggtgtga
      481 acgtcacggc agcgagcagc cgcgtctcgc gatcgtcgag cagcgcggcg tcggtcagcg
      541 tgccgggcgt gacgatgcgc acgacgtcgc gcgcgacggg ccctttgctc gtggcggggt
      601 cgcccatctg ctcgcagatc gcgaccgact cgccgagccg cacgagcctc gcgagatacg
      661 tctcgacgga atgcacgggc acgccggcca tcgggatcgg ctcgccgccg gactggccgc
      721 gccgcgtcag cgcgatgtcg atgagcttcg cggtgcgccg cgcatcgtcg tagaagagct
      781 cgtagaagtc gcccatgcga tagaacagca gcgtgtcggg aaacctcgcc ttgatggcgt
      841 ggtactgctg catgacgggc gtgtgctgcg cggcgctcat cttcaaaaag gggacagatc
      901 tattttctgt ttctaaatgg ggacggatct attttctgtt ctgagcgccg agattgtcag
      961 ctggtctgct cacgaaatac agaagaatct tccgcttgac gattgcagct ggagggggcc
     1021 gatgccgagg tctgcgcgag tggtggtgcc ggcgcatgcg tatcacgtct ttcaacgcgg
     1081 ccacaaccaa caagcctgct tcgctgagac cggcgattac gagcggtacc tcgcgaacct
     1141 cgcggagctc aaagaagagc tgggcgtaaa agtgtacgca tggtgtctga tgaccaacca
     1201 tgtgcatttg ctactggagc caagcactgc gacaagcctt gggttgctca tgaagcggtt
     1261 gtccggtcga cagactcggt atcacaatcg attggaacag cgcagcggca cgctttggga
     1321 aggtcgctac aaatcgagct tggtgcaaag tgaagcctat cttctggcct gttgccgcta
     1381 catcgagctc aatccagtac gcgcgtgcat agtggcagcg cccgaagact accgatggtc
     1441 tagctgtcga gcgcggctgg ggtatgcgag gtccaccatt ctcgatctgg atccgtgcta
     1501 tcaagagtta gcggcggacg agtcggtgcg gcgtgagcgg tacagggaat tcttgcaaag
     1561 tgcgattccg gagggggagt gggcactgat tcgcaccgcc gtccggcgcg gtcaactcac
     1621 cgggaatgaa gccttccgcc agcaagtcgc tgcaactctt caccggagaa tcgagaatcg
     1681 tgggccgggg cgaccggcga gaaaatagat ctgtccccat tttgctcgaa tctattctcc
     1741 gatatctccg gacagtagtg gggtctgtcc ccttttaggg gttcattgcc tggtgtagcg
     1801 gacgaacgag cgtgggccga cttcggcgcc gtagacgacg gcgccgccgt tgatggcggc
     1861 gacgccttcc gcggcgctgc tgccgccctg cgggtcggga tccggaatga aataccggac
     1921 cgagccgtcg cgcgcgctgc cgatccggat gccgcgctgc cagcccgggt gagcgaggtc
     1981 gcggccttcg ttcacgccgt cgcgcgattc cgagtcggcg acgaacaagt tgtcgtcggc
     2041 gtcgatgtag atgccgctgg gacggctgaa ctgataccag cagtcgaggt agttgccgtc
     2101 ctgatcgtag atctggatgc ggttgttgtc gcgatcgccg atgaagagcc gcccgcgcga
     2161 atcgatcgcg atcgcgtgca ggctcgagaa ctcgccggcg ccgcacggcc cgttgccggc
     2221 cttgccccac gacttgatga acttaccgtc cttgtcgaac ttgatgatgc gctcgctcgt
     2281 gccttcgccg tgcccgtccg cgacgaagat gtcgccattg cgaccgacca caacgtccga
     2341 cggcgagctg aagtgatccg gcgggtcgcc cgcgaccttg cgcgtgccga gctgcagcaa
     2401 gacctcgccc gacgggctca gcttcgtgac ctgctgcccc acgttgccgc ccgcgccgcg
     2461 gccgtcgggc ggcagcgggt ccgtgaccca gacgttgccg tcgtcgtcga cgtggatgcc
     2521 gtgcgggaac gcgaacaggt cggcgcccat ctggccgatc aggcgaccgg cggcgtcgta
     2581 gtgcaacagc ggattcgcgt gcgagccgac gcacgtgttg ctgccgcagc ggtcgatcgc
     2641 ccagacgtcg tcgcggccct tggccgcatc gacgccggcc gtcgagcccc acgtgcggcc
     2701 gtcgggcagc ggcatgactt gccggtccga gtacggattc ggcaggtcgt tgacgggcgg
     2761 catgttcgga tcgctgaa
//
LOCUS       DASSNN010000664         6385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_347916, whole genome shotgun sequence.
ACCESSION   DASSNN010000664 DASSNN010000000
VERSION     DASSNN010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347916"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2719
                     /locus_tag="VFL84_12385"
     CDS             <1..2719
                     /locus_tag="VFL84_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7609469.1"
                     /translation="TTDAGDGDNVAMSVAGGLDIGERWSFIGSFESQRIDQIQRNPDE
                     VGDWYRRWGWVQNPAWSSASPAAVPRNLKLPDVHSTVHAPTGKIDKFLSGGTVVAPTF
                     TFNGQSPVGQVFTMDGTGLVPFAPGTVVGCGATAACLTTGGAAGATTGTGSGGTGSQS
                     GGPEAAIANDAFNGGPYGAEVRRDNIFAGFTFDATDNMRFFSNLLLGSTESNNLDDRG
                     IPHGTSPWHYQIFSDNAYLPTALRQAMVAQGLTSFTLQKQGTVLGMPGNYNDHEERRN
                     EFETWTLQLGLDQQINDNWRMQARLQRGSTRKYTAVLNELRVDREYLGMDAVEVYTDR
                     RDVNGDGVVDLIAEPDRGTGTIVCNVQRYNVTDNDPRLAASVAGFRVPSVQGDDSLGG
                     PTDLVPIPGPVGPDAISNCVPFNVLGQGNVSEAAQDYLTSIKWGDSVVTQEFAEVLFT
                     GDVWEGFGPGAFSIATGLTYRDQWFWQRGQPQALMAYGPPRNADGSPAANGVNIGIRG
                     IPAGFTGGSANLHEFSTVPVIKGGFDVSEAFAEINMPLWDGGPRRLEVDVAGRHSDYS
                     TSGGIDSYKVGVNLQIAEFLRFRATNSRDVREPTFSERFDLQGGGGRVTDPLNGNASV
                     EITTVSGGNPNLAPEEADTNTAGFIFQPAKIPGFQFSVDWYEIDLSEAVGQLGAQLIV
                     NECAAGVPGRCELVTRDPLTNAITSVRNVFLNIDRAKVRGIDYEVLFNAEPNLASNQA
                     EALTFRLLAGRLLEDSTTSRTATGGFATTDNSNLYTEPDLEVLASVRYQIGAFGIGVQ
                     QRYLPESKLNVQWLEWYPGIVLPTANTITVDDNTVEGQTNTDLTFTYDATARGDNARW
                     SLSLAISNVFDVDPPVVADFGQRFSAQTIPVNSFDVYGRRYLMSFDYKF"
     gene            2785..3309
                     /locus_tag="VFL84_12390"
     CDS             2785..3309
                     /locus_tag="VFL84_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HET7609470.1"
                     /translation="MGGVLGVAAALWGQVTMGQASDDAAPALVFAFELRAQVAAPVEI
                     GEVPHGRRRIVAILGGTVKGPQLNATVLPGGADWQLIQRDGFSELDTRYTLETDKGQL
                     VYVQNAGIRHAPPDVMQKLLAGETVDPKLVYFRTVPKFETAAPELQWLARSVFVGVGE
                     RFPTEVVVRFYRLE"
     gene            3306..4712
                     /locus_tag="VFL84_12395"
     CDS             3306..4712
                     /locus_tag="VFL84_12395"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7609471.1"
                     /translation="MSRKRTLSRRSLLRAGAAAAGGAAALAQAGAAFGQAPAAVTQRR
                     FRAWISRGEGPGRTTLQEATLRPITGRQVLVRTEATNLCYTLVPAVLGLAAPAAPAPQ
                     AAPVAGALAAPPSRRRINDMAVIQGHGGVGVVEAVGPDVRRVQVGDRVCVSGTPHCGA
                     CYRCLRGRSDMCQFLSAIGPDDLVPIADLADGTPVYENSHIGGLAEVMVAFEEWVVPI
                     FTTKPAAEVGMVCGCTSVAGLGATTAHGLVAIEPAASVAVVGCGPIGLSAVQGARIAG
                     ASTIIAIDPIAARREMARKVGATHALDPNAEGDGLVDKVRSLTTWPTDRLWAGGRNPG
                     GRRPGSGADFVIEAAGADNVTPRAERGPDSTGVRAMQQAYRMTALGGHLITTGLLRGD
                     IVLPGNLFSFGGVTHHSGQAGGCSPMRDIPRFVELLEKGQFDARSLATGIVPLERMLE
                     GYEQVAYRTTITGIMTAS"
     gene            4808..5938
                     /locus_tag="VFL84_12400"
     CDS             4808..5938
                     /locus_tag="VFL84_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7609472.1"
                     /translation="MRAWLLGFVVLVATGASAAAATRQPAFDESELASGAALALAPPE
                     RPIMIPSFDDTFALDAEMESFVAPLKEVREPRQRMQALVEALEARGMFSLEYAEVTRT
                     ARDTFHDRQGNCLSFTMLFVSLARAIGLQASYQSVVVPPTWSNDGQVVVASHVNTAVR
                     TGRGEETIVDFNLRPYQAEHRSRRVGDSYALALFYTNLGAEAMLRNDHPAALAYLREA
                     ARVRPDIAGVWVNLGVLYARHGRYEHAEAAYLHALDVDDDEPSALTNLALVYSALGEH
                     ELATQYHDRVQSYRERNPYYHFASAARAYDNQRFADALTSLRKALRLKPDEEEFYELR
                     GKVQTALGKSRDATQSFELARSYAEAEELRSRSAVELGIGLQ"
     gene            complement(5946..>6385)
                     /locus_tag="VFL84_12405"
     CDS             complement(5946..>6385)
                     /locus_tag="VFL84_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609473.1"
                     /translation="SYDPKAPPSRQFGNPVHCVRPTHDGLVYVCDRVNDRVQIFRKDG
                     TFVSEAFFERDTLLSGSVSELAFSPDPNQTFMYMVDGVNNELRIVERATNEVLARVGR
                     PGRYAGQFHVVHNVAVDSEGNVYTTEVNTGQRVQKFRRLDPQD"
BASE COUNT         1051 a         2142 c         2156 g         1036 t
ORIGIN      
        1 cacgacggac gccggcgacg gcgacaacgt cgccatgtcg gtggccggcg gtctcgacat
       61 cggcgagcgc tggtcgttca tcggctcgtt cgagagccag aggatcgatc aaatccagcg
      121 caatcccgac gaagtcggcg actggtaccg gcgctggggc tgggtacaga accccgcttg
      181 gagctccgct tcgccggccg ccgtgccccg taacctcaag ctgcccgacg tccattccac
      241 ggtgcacgcg cctacgggca agatcgacaa gttcttgagc ggcggcaccg tcgtggcgcc
      301 aacgttcacg ttcaacggcc agagccccgt aggccaagtg ttcacgatgg acggcaccgg
      361 cctcgttcct ttcgcgccgg gtaccgtcgt cggctgcggc gcgacggcag cgtgtctgac
      421 gaccggggga gcggccggcg cgacgacggg caccggcagc ggcggcaccg gctcacagtc
      481 aggtggtccg gaagccgcca ttgcgaacga cgcattcaac ggagggccgt acggcgccga
      541 ggtcaggcgc gacaacatct tcgcgggctt cacgttcgac gcgaccgaca acatgcgctt
      601 cttcagcaac ttgcttttgg gttcgaccga gtcgaacaac ttggacgacc gcggcatccc
      661 gcacggcacg tcgccgtggc actaccagat cttcagcgac aacgcgtatc tgccgacggc
      721 gctgcgccaa gcgatggtcg ctcaggggct tacgagcttc acgctgcaga agcagggcac
      781 ggtcctcggc atgccgggca actacaatga ccacgaggag cggcgcaacg agttcgagac
      841 ctggacgttg cagctcgggc tcgaccagca gatcaacgac aactggcgca tgcaggcgcg
      901 cttgcagcgg ggctcgacgc gtaaatacac ggccgtgctg aacgagctgc gcgtcgaccg
      961 cgagtatctc ggcatggacg ccgtcgaggt ctacaccgat cggcgcgacg tcaacggcga
     1021 cggcgtcgtc gatctgatcg ccgagcccga tcgaggtacg ggcaccatcg tctgcaacgt
     1081 ccaacgctac aacgtcaccg acaacgaccc gcgcctggcg gcgtcggtcg cgggcttccg
     1141 cgtgccgtcc gtgcaaggcg acgactcgct tggcgggccg accgatctcg tgccgatccc
     1201 gggccccgtc gggccggacg caatctcgaa ttgcgtgccg ttcaacgtgc tcggccaagg
     1261 caacgtctcc gaggcggcgc aggattatct gacgtcgatc aagtggggcg actcggtcgt
     1321 gacgcaggag ttcgcggaag tcttgttcac cggcgacgtg tgggagggct tcggtccggg
     1381 agcgttctcg atcgcgacgg gtctcaccta tcgcgaccag tggttttggc agcgcggcca
     1441 accgcaggct ctgatggctt acggtccgcc acgcaacgcc gacggctctc cggccgcgaa
     1501 cggcgtcaac atcgggattc gcggcattcc ggccggcttc accggcggca gcgccaacct
     1561 ccacgagttc tcgacggtgc cggtcatcaa aggcgggttc gacgtcagcg aggcgtttgc
     1621 cgagatcaac atgcccctct gggatggggg gccgcgtcgg ctcgaggtcg acgtcgcggg
     1681 tcgacactcg gattattcga cgagcggcgg gatcgattcc tacaaggtcg gcgtgaacct
     1741 ccagatcgcc gagtttctgc gcttccgcgc cacgaactcg cgcgacgttc gcgagccgac
     1801 cttttcggaa cgcttcgacc tgcaaggcgg cggcggtcgc gtcaccgatc cgctgaacgg
     1861 caacgcctcg gtcgagatca cgaccgtcag cggcggcaat cccaacctcg cgccggagga
     1921 agccgacacc aatacggccg gcttcatctt ccagccggcg aagattccgg gatttcagtt
     1981 ctcggtcgac tggtacgaga tcgatctcag cgaagcggtc ggccagctcg gcgcgcagct
     2041 gatcgtcaat gagtgtgcgg ccggggtccc cggccggtgt gagctcgtca cgcgcgatcc
     2101 tttgacgaac gcgatcacca gtgtccgcaa cgtgttcctg aacatcgatc gagcgaaggt
     2161 acgcggaatc gactacgagg tgctgttcaa tgccgagccg aacctcgcgt cgaaccaggc
     2221 cgaagcgctg acgttcaggc tcctggccgg caggttgctc gaggacagca cgacctctcg
     2281 gacggccaca ggcggtttcg cgacgacgga caactccaat ctctacacgg agcccgacct
     2341 cgaagtgctc gcgtcggtgc gctaccagat cggtgcattc ggcatcggtg tgcagcagcg
     2401 ctacctgccg gagtcgaagc tgaatgtgca gtggttggaa tggtacccgg gcatcgtgct
     2461 gccgaccgcc aacacgatca ccgtcgacga caacacggtc gagggccaga cgaacaccga
     2521 cctcacgttc acgtacgatg cgaccgcgag aggtgacaat gcgcgttggt ctctgtcgct
     2581 cgcgatcagc aacgtgttcg acgtcgatcc gcccgtcgtc gccgatttcg gtcagcgctt
     2641 cagcgcgcag acgatcccgg tgaacagctt cgacgtgtac ggccgccggt acttgatgag
     2701 cttcgactac aagttctgag acccttcggc cgcgcagcgg aacgcgctag gatctcgccc
     2761 atggtaaacg gatcttctcg gatcgtgggc ggggtcctcg gcgtggccgc ggccctctgg
     2821 gggcaggtca ccatgggtca ggcaagcgat gacgccgctc cggcgctcgt gttcgcattc
     2881 gagctgcgcg cgcaagttgc cgctccggtc gagatcggcg aagtcccgca cggccggcgc
     2941 cgcatcgtcg cgatcctcgg cggcaccgta aaggggccgc agctcaatgc caccgtcctg
     3001 ccgggcggcg cggactggca gctgattcaa cgcgacggct tctcggagct cgacacgcgc
     3061 tacacgctcg agaccgacaa aggtcaactc gtctacgtgc agaacgccgg gatccgtcac
     3121 gcgccgcccg acgtcatgca gaagctcttg gccggcgaga ccgtcgatcc gaagctcgtg
     3181 tacttccgca cggtgccgaa gttcgagacg gcggcgccgg agctgcagtg gctcgcgcgc
     3241 agcgtcttcg tcggggtcgg cgagcgcttc ccgacggaag tcgtcgtgcg gttctatcgc
     3301 ctcgaatgag caggaagcgc acgctctctc gccgcagctt gctgcgcgcc ggcgccgcgg
     3361 cggccggcgg cgccgccgca ctcgcgcagg ccggcgcggc gttcggccag gcgccggcgg
     3421 ccgtcacgca gcgccggttc agggcctgga tctcgcgcgg cgaaggaccc ggccgcacga
     3481 cgttgcagga agcgacgttg cggccgatca cgggacgcca agtgctcgtg cgcaccgaag
     3541 cgacgaatct ttgctacacg ctcgtgccgg ccgtgctcgg ccttgccgcg ccggcggctc
     3601 ccgcgccgca agcggcgccc gtagcgggcg cgctggccgc gccgccgagc cggcggcgca
     3661 tcaacgacat ggccgtgatt caaggccacg gcggcgtcgg cgtcgtcgaa gccgtcgggc
     3721 ccgacgtgcg ccgcgtgcaa gtcggcgacc gcgtctgcgt ctcgggcacg ccgcactgcg
     3781 gcgcgtgcta tcgctgcctg cgcggccgct ccgacatgtg tcagttcttg agcgcaatcg
     3841 gtcccgacga tctcgtcccg attgcggatc tcgcggacgg cacgccggtc tacgagaact
     3901 cgcacatcgg cgggctcgcc gaagtcatgg tcgcgttcga agaatgggta gtgccgatct
     3961 tcacgacgaa gcctgcggcc gaggtcggca tggtctgtgg ctgcacgagc gtcgccggcc
     4021 tcggcgcgac gaccgcgcac gggctcgtcg cgatcgagcc cgcggcgagc gtcgccgtcg
     4081 tcggctgcgg gccgatcggc ttgtcggccg tgcagggcgc gcgcatcgcc ggcgcttcga
     4141 cgatcatcgc gatcgatccg atcgccgcgc ggcgcgagat ggcgcgcaaa gtcggcgcga
     4201 cgcatgcgct cgatccgaac gccgaaggcg acggcctcgt cgacaaggtt cgttcgctga
     4261 cgacgtggcc gacggaccgg ctctgggcgg gcgggcgcaa cccgggcggc cgccgtcccg
     4321 gctccggcgc cgacttcgtc atcgaggccg cgggggccga caacgtaacg ccgcgcgccg
     4381 agcgcggccc cgattccacg ggtgtgcgcg cgatgcagca ggcgtatcgc atgacggcgc
     4441 tcggcggtca tctgatcacg accgggctgc tgcgcggcga catcgtgcta cccggcaatc
     4501 tgttctcgtt cggcggcgtc acgcatcaca gcgggcaggc cggcggctgc agcccgatgc
     4561 gcgacatccc gcgtttcgtc gaattgctcg agaaaggcca gttcgacgcg cgctcactgg
     4621 cgacgggcat cgtgccgctc gagcgcatgc tcgagggtta cgagcaggtc gcgtatcgaa
     4681 cgacgatcac gggaatcatg actgcctctt gagaggcgtt gccgcagcgc gacataaggc
     4741 ctgcggcacg agttggcggc cggcgtcggc ggagtacaat cgtggtgtcc cgctgcgggt
     4801 ggcgtcgatg cgagcttggc tcctcggttt cgtcgttctc gttgcgaccg gagccagcgc
     4861 ggctgcggcc acccgccaac ctgcattcga tgaaagcgag ctcgcgtcgg gcgccgcgct
     4921 cgcgctcgcg ccgcccgagc gcccgatcat gatcccgtca ttcgacgaca cctttgcgct
     4981 cgacgcagag atggaatcgt tcgtcgcgcc gctgaaggaa gtgcgcgagc cgcggcagag
     5041 gatgcaggcg ttggtcgaag cgttggaagc gcgcggcatg ttctcgctcg aatacgcgga
     5101 ggtcacgcgc acggctcgcg acacgttcca cgatcggcag ggcaactgcc tttcgttcac
     5161 gatgctgttc gtctcgctcg cgcgcgctat tggcttgcag gccagttatc agagcgtcgt
     5221 ggtgccgccg acttggtcga acgacggtca ggtcgtcgtg gcgagccacg tgaatactgc
     5281 cgtgcgcacg ggccgcggcg aagaaacgat cgtcgacttc aaccttcgcc cctatcaggc
     5341 cgagcaccgc agccggcgcg tcggcgattc gtacgcgctc gcgctgttct atacgaacct
     5401 cggcgccgaa gcgatgctgc gaaacgatca ccccgcggcg ctcgcctatt tgcgcgaagc
     5461 cgcacgcgtc cgtcccgaca tcgcgggcgt ctgggtcaac ctgggggtgc tatacgcgcg
     5521 ccacggccgg tacgagcatg cggaggccgc ttatctgcat gcgctcgacg tcgacgacga
     5581 cgagccctcc gcgctgacca acctcgcgct cgtgtactcg gcgctcggcg agcacgagct
     5641 agccacccag tatcacgatc gcgtgcaaag ctatcgcgag cgcaacccgt actaccactt
     5701 cgcgtcggca gcgcgagcct acgacaatca gcggttcgcg gacgcgttga cgtcgctgcg
     5761 caaggcgttg cgcctgaaac cggacgaaga ggaattctac gagctgcgcg gcaaggtcca
     5821 gacggcgctc ggcaagtcgc gcgacgcgac gcaaagcttc gagctcgcgc gctcctatgc
     5881 ggaagcagag gagcttcgct ctcggtccgc cgtggagctc ggcatcggct tgcagtagcc
     5941 cttcgtcagt cctgcggatc gagccggcgg aatttctgca cccgctgccc cgtgttgacc
     6001 tcggtcgtgt agacgttgcc ctccgagtcg acggccacgt tgtggacgac atggaactgc
     6061 cccgcgtagc ggccgggccg gccgacgcgc gcgagcactt cgttcgtggc gcgctcgacg
     6121 atgcgcagct cgttgttgac gccgtcgacc atgtacatga acgtctgatt aggatcgggc
     6181 gagaacgcga gctcggacac ggagcccgac agcaacgtgt cgcgctcgaa gaacgcctcc
     6241 gatacgaacg tgccgtcctt gcggaagatc tgcacgcgat cgttgactcg gtcgcagacg
     6301 tacacgaggc cgtcgtgcgt cggccgcacg cagtgcacgg gattgccgaa ctgccgagac
     6361 ggcggcgcct tcggatcgta ggacg
//
LOCUS       DASSNN010000665         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1497870, whole genome shotgun sequence.
ACCESSION   DASSNN010000665 DASSNN010000000
VERSION     DASSNN010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497870"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFL84_12410"
     CDS             complement(<1..199)
                     /locus_tag="VFL84_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609474.1"
                     /translation="MDLRRRRPPPAALALLGLAAALTAPRLGRAQEPPATSPTPAETA
                     VNSPMVAGESEADEPARQFVKW"
     gene            complement(223..1293)
                     /locus_tag="VFL84_12415"
     CDS             complement(223..1293)
                     /locus_tag="VFL84_12415"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012849011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II asparaginase"
                     /protein_id="HET7609475.1"
                     /translation="MRLVKGISATLAVVALAAAGQAAAQQQRKPNIVILATGGTIAGA
                     AASGTQAGYTSGAVTIDAMLKAVPGATDLANIKGEQIANVGSQDMSFEILLKVAKRIN
                     ELLPSPDVDGIVITHGTDTLEESAFFLNLVVKSDEPVVMVGSMRPSTAVSADGPLNLY
                     NAIAVAADPAAKARGVLVVMNDWIHLAHSLTKTSTTAVQTFLSPVRGLGGIVAYGKTD
                     WYNIPAWKHTTRSEFDIARVNALPRVDIVYAYADMPADAIDSAVKNGAKGIVIAGVGN
                     GNMNAASVQAASKAAKSGVAVVRSSRVATGSVGRNVELNDDQLGLIASDELNPQKARI
                     LLTLALLQPHTPEQLQQLFYTY"
     gene            complement(1326..>2208)
                     /locus_tag="VFL84_12420"
     CDS             complement(1326..>2208)
                     /locus_tag="VFL84_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HET7609476.1"
                     /translation="LVIPKMGRTDLQDAVPMTVGQEFHAFAAALESEIALLRDAEKYL
                     YTINMGATAIGSEINVPDGYVEAVTKELAALTKKPIVPAKDMFAATWDQQAFVVYSSA
                     LKSVAVKLSKISSDLILLTSGPRAGLAEIKLPERQPGSSIMPGKVNPVIPELMNVIAF
                     RVMGNDAGVAFAAHSGQLQLNAYEPLVGLNVMESQHILFTGSKALREQCVDGITVNQQ
                     VLTHYMETTVGIVTALNTVIGYEKATELAGKAYSSGKGILEIIREEKVLTDAQIKDLL
                     DPVKLTNLDRSLYPPTP"
BASE COUNT          331 a          711 c          748 g          418 t
ORIGIN      
        1 tccacttgac gaactggcgc gcgggctcgt cggcctcgct ttcgccggcc accatcgggg
       61 agttgacggc ggtctcggcc ggcgtcggcg aagtcgccgg cggctcttgc gcgcggccga
      121 gccgcggcgc cgtcagcgcc gcggccagcc cgaggagcgc gagcgcagcg ggcggtggcc
      181 gccgacgacg aaggtccatc taggcagtcg acgcgcgcgc gatcagtacg tgtagaagag
      241 ctgctggagc tgctccggcg tgtgcggctg cagcagcgct agggtcagca gaatccgcgc
      301 tttctgcggg ttcagctcat ccgaagcgat gaggccgagc tggtcgtcgt tgagctcgac
      361 gttgcggccg acggagcccg tcgcgacacg cgagctgcgc acgacggcga cgccgctctt
      421 ggctgctttc gacgcggcct gcaccgacgc ggcgttcatg ttgccgttcc cgacgccggc
      481 gatcacgatg cccttcgcgc cgttcttgac ggcggagtcg attgcatcgg ccggcatgtc
      541 cgcgtacgcg tagacgatgt cgacgcgcgg cagcgcgttc acgcgcgcaa tatcgaactc
      601 gctgcgcgtc gtgtgcttcc aggccgggat gttgtaccag tcggtcttgc cgtaggccac
      661 gatgccgccg agcccgcgca ccggcgacag gaacgtttgc acggccgtcg tgctcgtctt
      721 cgtcagcgag tgcgcgaggt ggatccagtc gttcatgacg acgaggacgc cgcgcgcctt
      781 cgccgccgga tcggccgcga cggcgatcgc gttgtagagg ttcagcgggc cgtcggcgct
      841 gacggccgtg gacgggcgca tcgagcccac catgacgacg ggctcgtcgc tcttgacgac
      901 gaggttcaag aagaacgccg actcctcgag cgtgtccgtg ccgtgcgtga tcacgatgcc
      961 gtcgacgtcc ggcgacggca gcagctcgtt gatgcgcttc gcgaccttga gcaggatctc
     1021 gaacgacatg tcctgcgagc cgacgttcgc gatctgctcg cccttgatgt tcgcgagatc
     1081 ggtggcgccg ggaaccgcct tcaacatcgc gtcgatcgtg acggcgcccg acgtgtagcc
     1141 cgcttgcgtg ccgctcgccg cggcaccggc gatcgtgccg ccggtcgcga gaatgacgat
     1201 gttgggcttt cgttgttgct gggcagccgc ctgccctgcg gcggcgagag cgacgaccgc
     1261 cagcgtggcc gagatacctt tgactagtcg catgtgcttc tcctagctcg cgtgcgcgta
     1321 cgcgctcagg gggttggggg gtagagactt ctgtcgaggt tcgtgagctt gaccgggtcg
     1381 agcagatcct tgatctgcgc gtccgtgagg accttctcct cgcggatgat ttcgaggatg
     1441 cccttgccgc tcgagtaggc ttttcctgca agctcggtcg ccttttcgta accgatgacc
     1501 gtattcaatg cggtcacgat gccgaccgtc gtttccatgt agtgcgtgag cacttgctga
     1561 ttgacggtga tgccgtcgac gcactgctcg cgcagcgcct tcgagccggt gaacagaatg
     1621 tgctgcgact ccatgacgtt gagcccgacc aacggctcgt aggcgttcag ctgcagctgc
     1681 ccgctgtgtg cggcgaaggc gacgccggca tcgttgccca tgacgcggaa cgcgatgacg
     1741 ttcatgagct caggaatcac gggattgact ttgccgggca tgatcgagga gcccggctgc
     1801 cgctccggca gcttgatctc ggcgaggccg gcgcgcggtc ccgacgtcaa caggatcaag
     1861 tcgctcgaga tcttcgagag cttgacggcg acgctcttca gcgccgacga gtagacgacg
     1921 aacgcctgct ggtcccaggt cgccgcgaac atgtccttcg cgggcacgat cggcttcttc
     1981 gtcagcgcgg cgagctcttt cgtgaccgcc tcgacgtagc catccggcac gttgatctcc
     2041 gagccgatcg ccgtggcgcc catgttgatc gtgtagaggt atttctcggc gtcacgcagc
     2101 aaagcaatct cgctctcgag cgctgcagcg aacgcgtgaa actcctgccc gaccgtcatc
     2161 ggcacggcgt cttgcagatc cgtgcggccc atcttcggaa tcacgagg
//
LOCUS       DASSNN010000666         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1244855, whole genome shotgun sequence.
ACCESSION   DASSNN010000666 DASSNN010000000
VERSION     DASSNN010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244855"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VFL84_12425"
     CDS             complement(<1..155)
                     /locus_tag="VFL84_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET7609477.1"
                     /translation="MKLKGSKTEQNLKDAFAGESQANRRYLYFAAKADVEGFNDVATV
                     FRSTAEG"
     gene            329..580
                     /gene="xseB"
                     /locus_tag="VFL84_12430"
     CDS             329..580
                     /gene="xseB"
                     /locus_tag="VFL84_12430"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET7609478.1"
                     /translation="MAATEKSNDLGGLEKSLEELEALVVRLESGDLPLEQALKEFEHG
                     VKLTRQCQAALQEAEQKVEILLKKTATAEPSPFTNGDEE"
     gene            complement(687..1952)
                     /locus_tag="VFL84_12435"
     CDS             complement(687..1952)
                     /locus_tag="VFL84_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET7609479.1"
                     /translation="MPERIGKYVIINEVGQGSTGRVFLSHDPYYGRDVAIKLYNIDTE
                     EDEQKARVTRKMFFNEAQMMGRLQHPNILPIYDAGEENGAYYVVTEHVHGARTLNAYC
                     RPDNLLRIDDVVEILFKAAKGLHYAHGRGVVHRDIKPSNIMLTLDNDVRIIDFGIALI
                     KDSNVSRIEGIAGSPSYMSPEQVQSAEITHKSDIYSLGAVMYELLTGFRPFRANSLSK
                     LLHQIVYATPAPIHTLREGIPEEIENVVMTALLKEPEKRFASAVELAGALTKLHQKLR
                     SEHDRLDRQEQFDVLRKLRFFHDFSHAEIWEVLRASEWCTYQPEDEIIREGELDDRFY
                     IIVNGSVSVESGRRQTGVLTDGDCFGEASYVRGARRTATIKAISPLTLVRVSSTLLEQ
                     SSSACQLRFNKVFLRSLIERLQGIAPAAT"
     gene            complement(2010..>2374)
                     /locus_tag="VFL84_12440"
     CDS             complement(2010..>2374)
                     /locus_tag="VFL84_12440"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HET7609480.1"
                     /translation="LALFELPVPWRVAAVLAVIGAGIWICGESARRLQRHDDQRIVFD
                     EIAGVLLTSLAVAKPTVFALALVFVLFRVFDIVKPWPIRDVDHSLHGGLGIMLDDLIA
                     ALYAAACVATIRVLLPTI"
BASE COUNT          450 a          725 c          742 g          457 t
ORIGIN      
        1 tcgccttcgg ccgtcgaccg aaacacggtc gcgacatcgt tgaagccttc gacgtcggcc
       61 ttcgccgcga agtagagata gcggcgattc gcctgcgatt cgccggcaaa ggcgtccttc
      121 aaattctgtt cggtcttact gcccttgagt ttcatctgtg gtctccgaga acgtggattc
      181 gcaccggcgc cgacgtccgg cgaacagtcc ttagactttg tctaggaact cggacccaat
      241 ctattctggt cgccgaaaac ttgtcaaccc gtgcgtttga cccgggtttg ggcgccctgt
      301 accgtggccg cctcgctgaa ggaccgctat ggccgcgacc gaaaagagca atgacttagg
      361 tgggctcgag aaatcgctcg aggagctcga agcgctcgtc gtgcgcctcg agagcggcga
      421 cctaccgctc gagcaagcgc tgaaagaatt cgagcacggc gtcaagctga cgcgtcaatg
      481 tcaggcggca ttgcaggaag ccgagcagaa ggtcgagatc ctgctcaaga agacggccac
      541 ggccgagccg agcccgttca cgaacggcga cgaagaatga aaaaggggac agatcggttt
      601 gaaatgggga cagatctatt ttcggtcctc agaaaataga tctgtccccg ttttcatgtc
      661 ctcagaaaat agatctgtcc ccgttttcag gtggcggcgg gggcgatgcc ttgcagacgc
      721 tcgatcaacg agcgcaggaa caccttgttg aaccgcagct ggcacgccga cgacgattgc
      781 tcgagcagcg tcgagctcac gcgcacgagc gtcagcgggc tgatcgcctt gatcgtggcc
      841 gtgcggcgcg cgccgcgcac gtagctcgct tcgccgaagc agtcgccgtc ggtgagcacg
      901 ccggtctggc ggcgccccga ctccacgctg accgagccgt tgacgatgat gtagaaccgg
      961 tcgtcgagct cgccctcgcg gatgatctcg tcctcgggct ggtacgtgca ccattcgctc
     1021 gcgcgcagca cttcccagat ctcggcgtgc gagaagtcgt ggaagaagcg cagcttgcgc
     1081 aacacgtcga actgctcctg gcgatcgaga cggtcgtgct cgctgcgcag cttctggtgc
     1141 agcttcgtca gcgctccggc gagctcgacc gcgctcgcga accgcttctc gggctccttg
     1201 agcagcgccg tcatgacgac gttctcgatc tcctcgggga tgccttcgcg cagcgtatgg
     1261 atcggcgcgg gcgtcgcata cacgatttga tgcagcagct tgctcaagct gttcgcgcgg
     1321 aacggccgga aacccgtcag cagctcgtac atgaccgcgc cgagcgagta gatgtcggac
     1381 ttgtgcgtga tctcggccga ctgaacttgc tccggcgaca tgtagctcgg gctgcccgcg
     1441 atgccctcga tgcgcgagac gttcgagtcc ttgatcaacg cgatgccgaa atcgatgatg
     1501 cggacgtcgt tgtcgagcgt cagcatgatg ttgctcggct tgatgtcgcg atggacgacg
     1561 ccgcggccgt gcgcgtaatg caatcctttc gcggccttga agagaatctc gacgacgtcg
     1621 tcgatacgca gcaagttgtc gggccggcaa tacgcgttca gcgtgcgcgc gccgtgcacg
     1681 tgctccgtga cgacgtagta cgcgccgttt tcctcgccgg cgtcgtagat cggcaggatg
     1741 ttgggatgct gcagccgccc catcatctgc gcttcgttga agaacatctt gcgagtgacg
     1801 cgcgccttct gctcgtcttc ctcggtgtcg atgttgtaga gcttgatcgc gacgtcgcgg
     1861 ccgtaatacg ggtcgtgaga aaggaacacg cggcccgtgc tgccctgccc cacctcattg
     1921 atgatgacgt atttgccgat gcgctccggc acgtcgcgag agccgcgcct gtcggcggct
     1981 agtggggctt ttgcctgggc catagaaagc taaatcgttg gcagcagtac tcggatcgtt
     2041 gcgacacacg ccgccgcgta gagcgcggcg attaggtcgt caagcataat tccgaggccg
     2101 ccgtggagac tgtgatccac atcgcggatc ggccagggct tcacgatgtc gaaaactcta
     2161 aacaaaacaa acactagcgc gagcgcgaaa accgtcggtt tcgcgacggc gaggctcgtc
     2221 aaaagcacgc cggcaatctc gtcgaagacg atgcgctgat cgtcgtgacg ctgaaggcgc
     2281 cgcgcgcttt cgccgcagat ccagattcca gcgccgatca cggcgagcac ggccgccact
     2341 cgccacggca ccggcaactc gaacagggcc aagg
//
LOCUS       DASSNN010000667         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1497976, whole genome shotgun sequence.
ACCESSION   DASSNN010000667 DASSNN010000000
VERSION     DASSNN010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497976"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2325)
                     /gene="hrpA"
                     /locus_tag="VFL84_12445"
     CDS             complement(<1..>2325)
                     /gene="hrpA"
                     /locus_tag="VFL84_12445"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HET7609481.1"
                     /translation="PEILRSSLAAVILQMAALALGDVAAFPFVDPPPPRAIADGYQLL
                     QELDAVDASHALTARGRELAKLPLDPRIGRIVLAARENDCLAEALVIASALSVPDPRE
                     RPLERAQAADQAHLAFRDARSDFLSLIALWEFFDSPAQKALSHRKRVEACRAHFVSFL
                     RMTEWRDVHAQLVAVLTEAGIRVDTRLPPAIDAARYAAIHRSLLAGLLGNIGVKSEPA
                     ATMGGQYDGARGLRFFLHPGSGLAKKPPKWVLAAELTQTSRLYARCAGCVEPDWIEAV
                     AGDRVVHEYFDARWDRDRGEVVARERVKLYGLTLVAGRPVSYGAIAPAEARAVFIREA
                     LVADELGVDAPFVAHNRALIAEIAALEHKARREDVLVDDETIAAFFAARIPDDVVSRA
                     TFERWRKTAERSDARTLFLTREVLMRHAADTVTEALFPATLHAGDGQFPLEYRFAPGH
                     PLDGLTLTVPLARLNQVDGDALSWLVPGLVREKVTLLLKALPKGIRNRLVPLPSTVTS
                     FLGQPRPANASLSAALAAYVTEQTGETTEARTFEAVDLPAHLRINVRVVDAAGAELAM
                     DRALAPLRERLQEAARLSFAAQGPAFERKGIRQWTFGELPETLAVRRNRQGSRRSGTD
                     DGTVTGYPALVDEGDDGVALSLLDTKAAADAATRKGVVRLIDLELGDALARMVKHAPA
                     WTAIGLALRAAGPLDRLQEDVRAAVADRAFLGEDPLPRDRDAFVAQVKRARARLPAVF
                     EGALLTLAAIASEYQALSAKLAAMPSSQRMLASEA"
BASE COUNT          359 a          815 c          825 g          326 t
ORIGIN      
        1 gcttcggatg ccagcatccg ttgtgaagaa ggcatcgccg cgagttttgc ggaaagcgcc
       61 tggtattccg aggcgatcgc ggcaagtgtc aggagcgcgc cttcgaacac ggccggcagg
      121 cgcgcacgcg ctcgtttgac ctgcgcaacg aacgcgtcgc ggtcgcgcgg cagcggatct
      181 tcgcccagga acgcccgatc ggcgaccgcg gcgcgaacgt cttcctgcag gcgatcgagc
      241 gggccggcgg cgcgcaatgc gagcccgatg gcggtccacg cgggcgcgtg cttcaccatg
      301 cgcgcgagcg cgtcgccgag ctctaagtcg atcaggcgca cgacgccctt gcgtgtcgcc
      361 gcgtcggccg ccgccttcgt gtcgagcagc gacagcgcaa cgccatcgtc gccctcgtcg
      421 acgagcgccg ggtatccggt cacggtgccg tcatccgtcc cggagcgccg gctcccttgg
      481 cggttgcggc gcacggcgag cgtttccggc aactcgccga acgtccattg gcgaatgcct
      541 ttgcgctcga acgccggccc ttgggcggcg aacgaaaggc gcgccgcttc ctgcagccgc
      601 tcgcgcagcg gcgcgagagc gcggtccatc gcgagctcgg cgcccgcggc gtcgacgacg
      661 cgcacgttga tgcgcagatg cgcagggagg tccacggcct cgaacgtccg cgcttccgtc
      721 gtctcgcccg tttgctcggt cacgtaagcc gcgagcgccg cggacagcga cgcgttcgcc
      781 ggtcgcggct gcccgagaaa cgacgtgacg gtcgaaggca gcggcacgag ccggttgcgg
      841 atccctttcg gcaatgcctt cagcagcagc gtgacttttt cgcgcacgag gccgggaacg
      901 agccacgaca gcgcgtcgcc atccacctga ttgaggcgcg cgagcggtac tgtcagcgtc
      961 agcccgtcca gcggatggcc cggcgcgaac ctgtactcga gcggaaattg gccgtcgccc
     1021 gcgtgcagcg tcgcgggaaa gagcgcctcc gtcaccgtgt ccgccgcgtg ccgcatcagc
     1081 acttcacgcg tcaggaagag cgtccgggca tcgctgcgct cggccgtctt gcgccagcgc
     1141 tcgaacgtgg cgcgcgaaac gacgtcgtcc gggatgcgcg cagcgaaaaa cgcggcgatc
     1201 gtctcgtcgt cgacgagcac gtcctcgcgc cgcgccttgt gctcgagcgc cgcgatttct
     1261 gcgatcagcg cgcggttgtg cgcaacgaac ggcgcgtcga cgccgagctc gtcggcgacg
     1321 agcgcctcgc gtatgaacac ggcgcgtgct tcggcgggcg cgatcgcgcc atacgaaacg
     1381 ggccggccgg cgacgagcgt caggccgtag agcttcacgc gctcgcgcgc gacgacctcg
     1441 ccgcggtcgc gatcccagcg cgcatcgaaa tattcatgca cgacgcggtc acccgcgacc
     1501 gcctcgatcc agtcgggctc gacgcagccg gcgcaacgcg cgtagagccg cgacgtctgc
     1561 gtgagctcgg ccgcgagcac ccatttcggc ggtttcttcg cgaggcccga gcccggatgc
     1621 aggaagaagc gcaggccacg ggcgccgtcg tattgcccgc ccatcgtcgc cgccggctcc
     1681 gacttgacgc cgatgttgcc gagcagtccc gcgagcagcg agcggtgaat ggccgcgtag
     1741 cgcgcggcgt cgatcgcggg cggaagccgc gtgtcgacac ggattccggc ctcggtcagc
     1801 acggcgacga gctgcgcatg cacgtcgcgc cattcggtca tccgcagaaa cgacacgaag
     1861 tgggcgcggc acgcctcgac gcgcttgcga tgcgacagcg ctttttgcgc cgggctgtcg
     1921 aagaactccc acagcgcgat cagcgacagg aagtccgagc gcgcgtcgcg aaacgcgaga
     1981 tgcgcctggt cggccgcctg cgcgcgttcg agcggacgct cgcggggatc gggcaccgac
     2041 agcgcgctcg cgatcacgag cgcctcggcg agacagtcgt tctcgcgcgc cgcgagcacg
     2101 atgcggccga tgcggggatc gagcggaagc ttcgcgagct cgcggccgcg cgccgtcagc
     2161 gcatggcttg catcgacggc atcgagctcc tgcagcagct ggtagccgtc ggcgatcgcg
     2221 cgcggcggcg gcggatcgac aaacggaaac gcggcgacgt cgccgagcgc gagcgcggcc
     2281 atctgcagga tcaccgccgc gagcgagctg cggaggattt ccgga
//
LOCUS       DASSNN010000668         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1244942, whole genome shotgun sequence.
ACCESSION   DASSNN010000668 DASSNN010000000
VERSION     DASSNN010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244942"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /gene="kdsA"
                     /locus_tag="VFL84_12450"
     CDS             complement(<1..818)
                     /gene="kdsA"
                     /locus_tag="VFL84_12450"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HET7609482.1"
                     /translation="MEKKHVMIGDIDCGADRLFLIAGPCVIEEESLMMRTAERLKGIT
                     QRLGVPLIFKSSFSKDNRSSLEYYQGPGLEAGLRLLEKIKRQFELPILTDIHYPYQAA
                     PAAEVCDVIQIPAYLCMQSELVVAAAKTGAVVNIKHGQFLAPENMAKPVKKIEDSGND
                     RIIVTERGYTFGYNDLVVDPRSFYLLNQIGYPVVFDAGHSIRKYGIPSKDPRGSARQF
                     LTTLARSAVAAGVDGFFIESHPNPPEALCDAASQYALDDLESFMRPLIDIHNLVR"
     gene            complement(883..2172)
                     /locus_tag="VFL84_12455"
     CDS             complement(883..2172)
                     /locus_tag="VFL84_12455"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HET7609483.1"
                     /translation="MRFLLDFVYLIACILLSPWLVYRLCTAGRRDFAMRFGVGLRDSL
                     ASCIWLHGSSAGEVAVLRPLVAMLERDHPGTPLVISAFTATGLATARKLYARHRVVPL
                     PVDLSFVVARYLRSLNPRLLVVAESEFWPNLIGCTHSRGVPVALVNGKMSAKSFSLHA
                     RTRLVTRVLASFSMLAMQTEEHAQRLLALGVERARLHVTGNMKYDLARPSGDAASAAK
                     LRVALGFAQSDVVIIGGSLHDGEDEALLDAYAAARERVADAALVIVPRYPADADVIER
                     RARERGFAVTRKTAIDAGAAQALGNRAVLIVDTVGELARMYAVADLAFVGGSLFFRGA
                     NKGGHNLMEPAILGVPVLFGPYNFSFKETVEDLLAADAGKLVRDAAELRAVVASLVAD
                     RDARRELGSRARRVVEAKRGATARNYALLAEFARARP"
BASE COUNT          411 a          727 c          763 g          355 t
ORIGIN      
        1 cgaaccaagt tgtgaatgtc gatcagcgga cgcatgaagc tctcgagatc gtcgagagcg
       61 tattggctcg ctgcgtcgca gagcgcttcc ggcggattcg ggtgcgactc gatgaagaag
      121 ccgtcgacac ccgccgcgac cgccgagcgc gcgagagtcg tcaagaactg ccgcgcgctg
      181 ccgcgcggat ccttgctcgg gattccgtac ttgcggatcg agtggcccgc gtcgaacacg
      241 acggggtagc cgatctggtt caagagatag aagctacgcg gatcgacgac gagatcgttg
      301 tagccgaacg tgtacccgcg ctccgtgacg atgatgcgat cgttgccgga gtcctcgatc
      361 ttcttgacgg gcttggccat gttctcgggc gccaggaatt gcccgtgctt gatgttgacg
      421 acggcgcccg tcttcgcggc cgcgacgacg agctcggact gcatgcacaa gtacgcggga
      481 atctgaatga cgtcgcagac ttcggcggcc ggcgcggcct gatacggata gtggatgtcc
      541 gtgaggatcg gcagctcgaa ctgacgcttg attttctcga gcaagcgcag tccggcttcg
      601 agacccgggc cctggtaata ctcgagcgag ctgcgattgt ccttcgagaa cgacgacttg
      661 aagatcaagg gaacgccaag gcgctgcgtg atgcccttga gccgctcggc cgtgcgcatc
      721 atcagcgact cttcctcgat gacgcacggg ccggcgatca agaacagccg atccgcgccg
      781 caatcgatgt cgccgatcat gacgtgcttt ttttccatgt agcgtccaag tccttaaagt
      841 accgggcatt ctgggcccaa gggtcggcgg ctggcaatag actcaaggcc gcgcgcgcgc
      901 gaactcggcg agcagcgcat agttgcgcgc cgtcgcgcct ctcttggcct cgacgacgcg
      961 ccgcgcgcgc gacccgagct cgcgccgcgc gtcgcgatcg gcgacgagcg acgcgacgac
     1021 ggcgcgaagc tcggccgcgt cgcgcacgag cttgcctgcg tcggcggcga gcagatcctc
     1081 gaccgtttcc ttgaagctga agttgtacgg accgaacagc accggaacgc cgaggatcgc
     1141 gggctccatg agattgtggc cgcctttgtt cgcgccgcga aagaacaagc tgccgccgac
     1201 gaaggcgaga tcggcgacgg catacatgcg cgcgagctcg ccgacggtgt cgacgatgag
     1261 cactgcgcgg tttcccaacg cttgcgcggc gccggcgtcg atggccgtct tgcgcgtcac
     1321 cgcgaaaccg cgctcgcgcg cgcggcgctc gatgacgtcg gcgtcggccg gataacgcgg
     1381 cacgatgacg agcgccgcat ccgcgacgcg ctcgcgcgca gcggcatagg cgtcgagcaa
     1441 cgcctcgtcc tcgccgtcgt gcaggctgcc gccgatgatc acgacgtcgg actgcgcaaa
     1501 cccaagcgcg acgcgcagct tcgcggcgct cgcggcgtcg ccgctcgggc gcgcgaggtc
     1561 gtatttcatg ttgcccgtga cgtgcagtcg cgcgcgctcc acgccgagcg cgagcagacg
     1621 ttgcgcgtgc tcctcggtct gcatggcaag catcgagaag ctcgcgagca cgcgcgttac
     1681 gagccgcgtt cgcgcgtgca ggctgaacga cttcgccgac atcttgccgt tcacgagcgc
     1741 gacgggaaca ccgcgcgagt gcgtgcagcc gatcaagttc ggccagaact cggactccgc
     1801 gacgacgagc agccgtggat tcaacgagcg caggtagcgc gcgacgacga aagacaaatc
     1861 tacgggcagc ggcacgacgc ggtgccgcgc atagagcttg cgcgccgtcg cgagccccgt
     1921 cgcggtgaac gccgagatga cgagcggcgt gccgggatgg tcgcgctcga gcatcgcgac
     1981 gagcggcctc agcacggcca cctcgccggc cgaggagccg tgcagccaga tgcacgacgc
     2041 caacgagtcg cggaggccca cgccgaaacg catcgcgaag tcgcgtcttc ccgccgtgca
     2101 gaggcggtag acgagccacg gcgagaggag gatgcaggcg atgaggtaga cgaaatcgag
     2161 cagaaatctc atcgacaact tcgtgggccg cctcaacagg cgcccgaagt ttaccgcagt
     2221 cgcgaagcgg atgacctact tgcggcgggc tttctc
//
LOCUS       DASSNN010000669         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_113739, whole genome shotgun sequence.
ACCESSION   DASSNN010000669 DASSNN010000000
VERSION     DASSNN010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113739"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFL84_12460"
     CDS             <1..479
                     /locus_tag="VFL84_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HET7609484.1"
                     /translation="ARNRTPMRTVDLDVLSAENGASYWERDERHDLSVSLNRGQPALA
                     ALMRVMEDWIAHFLGVKVAIRAEREIDDAHWSWHVGLDAEASAILNDLYNRQPIDEAR
                     MGRLLCLFRLDFDNPADMRPALAGRPVWLAMAMDERNRLRLKPQNLLMNLPLAALQ"
     gene            543..1175
                     /locus_tag="VFL84_12465"
     CDS             543..1175
                     /locus_tag="VFL84_12465"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HET7609485.1"
                     /translation="MNESTTARDCSRARTALRLSAGVIGVLALGVAACAQQPAAPGPA
                     PAPPPAAPADAATPAVAPETSGPLAPLAWLEGCWQGNVNQREFREQWLPLRGGLLIGA
                     GQTVLHGKMQDYQYMRIEPKSDGVYFAQFSGDRKETSFRLAGTQTADKDTIFTFANTA
                     ADAFPARLIYRRGSEGWLYETIEGTLNGGERKVIYPLRRVDCETGEFILR"
     gene            1175..1531
                     /locus_tag="VFL84_12470"
     CDS             1175..1531
                     /locus_tag="VFL84_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7609486.1"
                     /translation="MADPNAPASKSAALASFERMLAAGKDSALLRYSIGNEHAKAKDW
                     PAAAEALARAVALDADYTAAWKLYARALLETGQPQAALEAYRQGIDVARRKGDRQAEK
                     EMAVFARRIERALAGG"
     gene            complement(1549..2076)
                     /gene="rnhA"
                     /locus_tag="VFL84_12475"
     CDS             complement(1549..2076)
                     /gene="rnhA"
                     /locus_tag="VFL84_12475"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HET7609487.1"
                     /translation="MSGVREKAGAPAAKPQATGDHSTAVVVYTDGACKGNPGPGGWGV
                     LLIRGGSEQELFGGERSTTNNRMELTAVIRALEALEQGSDVDLYLDSQYVKQGIESWI
                     HTWKRNGWRTADRKPVKNAELWRELDTLAKTHAIRWHWVRGHADTPGNHRADALANRG
                     VAQVNGAGNMNGATP"
     gene            complement(2073..>2325)
                     /locus_tag="VFL84_12480"
     CDS             complement(2073..>2325)
                     /locus_tag="VFL84_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7609488.1"
                     /translation="GRFDCYVPPFTQEKWLRRFAFFEKAGDRWWPITGGVYFLRATKK
                     VLGMRLLTPAWQRRERRKKALAPAAGARESLTTHRRRQR"
BASE COUNT          379 a          811 c          784 g          351 t
ORIGIN      
        1 tggcgcgcaa caggacaccg atgcgcacgg tcgacctcga cgttctgagc gccgagaacg
       61 gcgcgtcgta ctgggagcgc gatgagcggc acgatctgtc cgtgagcctc aaccgcggac
      121 agcccgcgct ggccgcgctg atgcgcgtca tggaggactg gatcgcgcac ttcctcggcg
      181 tcaaggtcgc gatccgagcc gagcgcgaga tcgacgatgc gcactggagc tggcacgtcg
      241 ggctcgacgc cgaggcgagc gcgattctca acgatctcta caatcgacag ccgatcgacg
      301 aggcgcggat gggacggctg ctgtgcctgt tccggctcga cttcgacaat cccgcggaca
      361 tgcggcccgc gctcgccggc cggccggtat ggctcgcgat ggcgatggac gaacgaaatc
      421 gcctgcgtct caagccgcag aacctgctga tgaatcttcc actcgcggcg ctgcagtgac
      481 cgcgacgccg atgaaccgcc gctggtcgat aattgcacct ccgacaccga ctcgagaacg
      541 agatgaacga atcgaccact gcacgcgact gctcacgtgc gcgaacggcg ctgcgcctct
      601 ccgctggcgt gatcggcgtc ctcgcgcttg gtgtagccgc gtgtgcgcag cagccggccg
      661 cacccggacc ggcgcccgcg ccgccgcccg cggccccggc ggatgcggca acgccggccg
      721 tcgccccgga aacgtccggc ccgctcgcgc cgctcgcatg gctcgagggg tgctggcagg
      781 gcaacgtgaa ccagcgcgag tttcgcgagc aatggctgcc gctgcgcgga ggcctgctga
      841 tcggcgccgg gcaaacggta ttgcacggca agatgcagga ctaccagtac atgcgcatcg
      901 agccgaaatc cgacggcgtc tatttcgcgc aattctccgg cgaccgaaag gagacgtcgt
      961 tccggctcgc cggcacgcag accgccgaca aggatacgat cttcacgttt gccaacaccg
     1021 cggccgacgc gtttccggcg cgcctcatct atcgccgggg gagcgaaggc tggctctacg
     1081 agacgatcga aggcacgctg aacggcggcg agcgcaaagt catctacccg ctgcgtcgcg
     1141 tcgactgcga aaccggtgaa ttcattctcc gctgatggct gatccaaacg cgccggccag
     1201 caagagcgcc gcactcgcga gcttcgagcg gatgctcgcc gcaggcaagg acagcgcgct
     1261 gctgcgctac tcgatcggca acgagcacgc gaaagcgaag gattggcccg cggccgcgga
     1321 ggcgctcgcg cgcgccgttg cgctcgacgc cgactacacg gccgcgtgga agctgtacgc
     1381 gcgcgccctg ctcgaaaccg ggcagccgca ggcggcgctc gaagcctatc ggcagggcat
     1441 cgacgtggcg cggcgcaagg gcgaccgcca ggccgaaaaa gagatggcgg tgttcgcgcg
     1501 ccgcatcgag cgcgcactcg caggcggctg aagtcgcgcg ggagccgttt acggcgtggc
     1561 gccgttcatg ttgccggcgc cattgacttg cgcgacaccg cgattggcga gcgcgtcggc
     1621 gcgatggttg ccgggcgtgt cggcgtgacc gcgcacccag tgccagcgga tcgcatgcgt
     1681 tttcgcgagc gtgtcgagct cgcgccagag ctcggcattc ttcacgggct tgcggtcggc
     1741 cgtgcgccag ccgttgcgct tccacgtgtg gatccagctc tcgatgccct gcttgacgta
     1801 ctgcgaatcg aggtagagat cgacgtccga cccctgctcg agcgcttcga gcgcgcggat
     1861 gacggcggtc aactccatcc ggttgttcgt cgtgctgcgc tcgccgccga aaagctcctg
     1921 ctcggagccg ccgcgaatca gcagtacgcc ccatccgccg ggccccgggt tgcccttgca
     1981 cgcgccgtcg gtatagacga cgaccgccgt cgagtgatcg cccgtcgctt gcggcttcgc
     2041 cgccggagcg ccggctttct cccgcacacc gctcaacgct gccgccgccg atgcgtcgtg
     2101 aggctctcgc gcgcgcccgc agccggtgcc agcgccttct tgcggcgctc gcgacgctgc
     2161 cacgcgggtg tcagcagccg catgccgagc accttcttcg tcgcgcgcag gaaatagacg
     2221 ccgccggtga tcggccacca gcggtcgccg gccttctcga aaaacgcgaa ccgtcgcagc
     2281 cacttctcct gcgtgaacgg aggcacgtag cagtcgaagc ggccg
//
LOCUS       DASSNN010000670         6763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1708432, whole genome shotgun sequence.
ACCESSION   DASSNN010000670 DASSNN010000000
VERSION     DASSNN010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708432"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2053
                     /locus_tag="VFL84_12485"
     CDS             <1..2053
                     /locus_tag="VFL84_12485"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HET7609489.1"
                     /translation="LGLAVGAIVVLLVVVAVAVSLLFDPNDYKDEITAAVQDATGRQL
                     TLDGDLELAVFPTIRIAVGSATLSNAPGFGDEPMARIGSASLSIALLPLLAQRIEVSR
                     ARLEGLTLNLARNRSGANNWQDLGGRDTPASAASAPAGGGGAPANLDLGVGAIEIENA
                     HIVYNDAAAGGRWELTGFGMTAEDFGLGDRFPLHMEFGLAGAEVAVQVAADMQATITL
                     ADNSYRLDDLAVTVDGSGAGWPGGPSQAKLNFDSLAANLADETLELNGLTLEFLGVTM
                     AGSLSGQKLLSNLALTGAVDIREFAPREVLERFGVDVQTADAAVLTRASAKANLLYNS
                     SQTGLRDMQFRLDDSTLTGRVALEGERITYDLTVDDINVDRYLPPASEESDAPAEEGS
                     LDEVDLPLEVMKTVDARGELKFGKAKFSGLTLTNVAFPLTVANGAARLTPSAQLYGGR
                     FSGDIRLTVQGNAANMSVEQTLDDVDLAGLGQDLLGSQDVTGTGDVRLKLATAGQNLG
                     AMRRDLDGDVAFSVTNGSLEGIDLWFELRRARARLDGETLPERGDAPRRTTFSSLSAT
                     GVVEDALLTNRDLNGTLDFMTVDGSGTVNLLDDTIAFDLVAKMVDGPVLRSDPAMVKY
                     AGKTLPLKVTGTIDAPSVLPDFSAIVRQQVTQEVEDKVDEEVDEAKERVRDRLRGLLN
                     R"
     gene            2050..2673
                     /locus_tag="VFL84_12490"
     CDS             2050..2673
                     /locus_tag="VFL84_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609490.1"
                     /translation="MRTAPAAAALGALGAGALAAAATIDDLDVTKQRGHYSLEANAYL
                     DATRESIFAVLTDFDDNAYSRISRAYKESRYLDEPAADGTPLVYTRLEGCMLWHCMTL
                     ERTERLETEAPGWIKSTALPEASNFKLATSEWVLEPDGDGTMMTYKLEMEPDFFVPPI
                     IGPWYLKRTLSQGGLRAVTRIERLARELDGRPNQPLPPLPPARTRDR"
     gene            2670..3719
                     /gene="mutY"
                     /locus_tag="VFL84_12495"
     CDS             2670..3719
                     /gene="mutY"
                     /locus_tag="VFL84_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET7609491.1"
                     /translation="MTTLAPRLLGWFDAHGRHDLPWQRDRTPYSVWVSEIMLQQTQVG
                     TVIPFYLRFMRRFPTSAALAAAPLDDVLAHWAGLGYYARARNLWRAARAVAAQHGGEL
                     PTTFDALHALPGIGRSTAGAILAQALGARWPILDGNVKRVLARYHAVAGWPGLPAVAN
                     ELWRHAETHTPHERVADYTQAIMDLGATLCARTRPACTVCPLTTGCEAALAGTQALYP
                     AARPKRERPQRRVAVLVVRMPDGRVLLERRPARGIWGGLYSLPELPAEDSPRDWCARR
                     LGAAVAAERALAIIEHAFTHFDLDLEPRLLELAAEPAAMMERDDWLWCRPGAKLDVGV
                     PAPVAALLSGPLGAA"
     gene            3727..3993
                     /locus_tag="VFL84_12500"
     CDS             3727..3993
                     /locus_tag="VFL84_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HET7609492.1"
                     /translation="MARKVNCVLLGTEAEGLDRAPYPGELGKRIYENVSKVAWQRWLK
                     HQTMLINEYRLTPIEPKARKFLEDEMQKFFFGQGSEAPPDYKPQ"
     gene            complement(4006..4371)
                     /locus_tag="VFL84_12505"
     CDS             complement(4006..4371)
                     /locus_tag="VFL84_12505"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7609493.1"
                     /translation="MKSYGIFYILAFVSDLTRSKAFYRDKIGCKLSTDEPRVAGLALG
                     EGYLVLHVDEREPKQRIYGGGMTVALQVEDAALEHARLAKLRVPVSKLIDQPWGERNF
                     TFTDPDGYVWLVAQSRGLS"
     gene            complement(4390..4965)
                     /locus_tag="VFL84_12510"
     CDS             complement(4390..4965)
                     /locus_tag="VFL84_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609494.1"
                     /translation="MPRTIAPIAAALLVLCPPAFAAAQSEPAATTETAAPAAERVEEI
                     VVEGRRTLFSLRRDVQAARENVWEVFSALNSDDDFDIACTSAPRTGSHVKNRACRPRY
                     ADNATRRAGQEVARRMSACGPSDSACLEAAMQMATGEAQAQLAIIPYMDRRLDDEFHR
                     LAADQPELAAAILEYLAKEHEYEDAVRSRGN"
     gene            complement(4988..>6763)
                     /gene="acs"
                     /locus_tag="VFL84_12515"
     CDS             complement(4988..>6763)
                     /gene="acs"
                     /locus_tag="VFL84_12515"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET7609495.1"
                     /translation="SFDANDLHVRWFADGELNVSVNCLDRHLAKRPEQTAILWEPDDP
                     NGSPRLISYRELHESVCKLANALKRLGVKKGDRVTIYMPMIPETAIAMLACARIGAIH
                     SVVFGGFSPEALKGRIEDCASRVVITADEGVRGGKKIPLKANVDEALGNVRGTKVEHV
                     VVVKHTGGNVGWTSGRDKWYEDVQDGASDLCPPETMNAEDPLFILYTSGSTGKPKGVL
                     HTTAGYLLHASITHQYVFDYRDGELFWCAADVGWVTGHTYIVYGALANGATTVMYEGI
                     PTYPDASRLWQVVDKHQIDILYMAPTAIRALMREGDGPVKKTSRASLRLLGTVGEPIN
                     PEAWEWYYHVVGDGRCPIVDTWWQTETGGMLITPLPGATKLKPGSATLPFFGVKPAIV
                     DPSGKILEGACSGNLVLLDSWPGQMRTVYGDHQRFVDTYFKHFPGMYFTGDGARRDED
                     GYYWITGRVDDVINVSGHRLGTAEVESALVGHAKVAEAAVVGFPHDLKGQGIYAYVTL
                     KAGESSSDALRKELVAWVRKEIGAIAQPDVIQWAPSRPKTRSGKIMRRILRKIAANDF
                     KELGDTSTLADPAVVDDLIKNRAAT"
BASE COUNT         1046 a         2282 c         2314 g         1121 t
ORIGIN      
        1 tctcggattg gcggtcggcg ccatcgtcgt tctgctcgtc gtcgtcgcgg tggcggtctc
       61 gttgctgttc gatccgaacg actacaagga cgagatcacg gcggccgtgc aggacgcgac
      121 gggccgtcag ctcacgctgg acggcgatct ggagctcgcc gtgttcccca cgattcgcat
      181 cgccgtcggc agcgcgacgc tgtccaacgc gcccggcttc ggtgacgagc cgatggcgcg
      241 catcggcagc gcgtcgttga gcatcgcctt gttgccgctg ctcgcgcagc gcatcgaggt
      301 cagtcgagcg cggctggagg ggctcacgct caatctcgcg cgcaaccgca gcggcgccaa
      361 caactggcag gacttgggcg ggcgcgacac gcccgcgagc gccgcgtccg ctccggcggg
      421 cggcggcggc gcgccggcca acctcgacct cggggtcggc gccatcgaga tcgaaaatgc
      481 gcatatcgtc tacaacgacg ccgcggcggg cggccgctgg gagctgaccg gattcggcat
      541 gacggccgag gatttcggtc tcggcgaccg cttcccgctg cacatggagt tcggcctcgc
      601 gggggccgaa gtggccgtgc aggtcgcggc cgacatgcag gcgacgatca cgctcgcgga
      661 caactcctat cggctcgacg atctggccgt gaccgtcgac ggcagcgggg cgggctggcc
      721 cggcggcccg agccaggcga agctcaattt cgactcgctg gccgcgaatc tcgcggacga
      781 gacgctcgag ctgaacggcc tcacgctcga gttcttgggt gtgacgatgg ccggctcgct
      841 gtccgggcaa aagctgctca gcaacctcgc gttgaccggc gccgtcgaca ttcgcgagtt
      901 cgcgccgcgc gaggtgctcg agcggttcgg cgtcgacgtg cagacggccg acgcggcagt
      961 gctgacgcgc gcgagcgcga aagcgaatct gctctacaac tcgagccaga ccgggctgcg
     1021 cgacatgcaa ttcaggctcg acgactcgac gttgaccggc cgcgtcgcgc tcgagggcga
     1081 gcgcatcacc tacgatctca ccgtcgacga catcaacgtc gaccgttatt tgccgcccgc
     1141 gtcggaggaa tcggatgcgc cggccgagga gggctcgctc gacgaggtcg acttgccgct
     1201 cgaggtcatg aagacggtcg atgcgcgcgg cgagctcaag ttcggcaaag cgaaattcag
     1261 cggcttgacg ctgacgaacg tggcgttccc gctgacggtc gcgaacggcg ccgcgcggct
     1321 cacgccgagc gcgcagctct acggcggccg gttcagcggc gacattcgct taacggtgca
     1381 gggcaacgcg gcgaacatga gcgtcgagca gacgttggac gacgtagacc tcgccgggct
     1441 gggtcaggac ttgctcggct cgcaagacgt cacgggcacg ggcgacgtgc gcctgaagct
     1501 cgcgacggcc ggccagaacc taggcgcgat gcggcgtgat ctcgacggcg acgtcgcgtt
     1561 cagcgtgacc aatggttctc tcgaagggat cgatctgtgg ttcgagctgc gtcgggcccg
     1621 cgcgcgcctc gacggcgaga cgctgcccga gcgcggcgac gctccgcgcc gcacgacgtt
     1681 ctcgtcgctg tccgccacgg gcgtcgtcga ggacgcgctg ctgacgaatc gcgatctgaa
     1741 cggcacgctc gacttcatga ccgtggacgg ctcggggacc gtcaacttgc tcgacgacac
     1801 gatcgcgttc gatctcgtcg cgaagatggt cgacggcccc gtgcttcggt ccgatcccgc
     1861 gatggtcaag tatgccggca agacgttgcc tctgaaggtc acgggcacga tcgacgcgcc
     1921 gtcggtgctt ccggattttt ccgccatcgt gcgccagcag gtcacgcagg aggtcgaaga
     1981 caaggtcgac gaggaagtcg acgaggcgaa ggaacgggta cgcgaccgcc tgcggggatt
     2041 gctgaaccgg tgaggacggc gcccgccgca gccgcgcttg gcgcgctcgg cgccggcgcg
     2101 ctcgcggccg cggcgacgat cgacgacctc gacgtcacta agcagcgcgg ccactattcg
     2161 ctcgaagcca atgcgtacct cgacgcgacg cgcgagtcga tcttcgcggt gctcaccgat
     2221 ttcgacgaca acgcgtattc gcgcatctcg cgcgcgtaca aagagagccg ctatctcgac
     2281 gagcccgccg ccgacggcac gccgctcgtt tacacgcgcc ttgaaggctg catgctctgg
     2341 cattgcatga cgctcgagcg caccgagcgg ctcgagactg aagcgccggg ctggatcaag
     2401 agcacggcct tgcccgaggc cagcaacttc aagctcgcca cgtcggaatg ggtgctcgag
     2461 ccagacggcg acggcacgat gatgacctac aagctcgaga tggagcccga cttcttcgtg
     2521 ccgccgatca tcgggccgtg gtatctgaag cgcacgctat cgcaaggcgg gctgcgcgcg
     2581 gtcacgcgca tcgagcggct cgcgcgcgag ctcgacggcc ggccgaacca gccgttgccg
     2641 ccgcttccgc cggcccgcac gcgcgatcga tgacgactct ggcgccgcgc ttgctcggct
     2701 ggttcgacgc gcatggccgg cacgacttgc cgtggcagcg cgaccgcacg ccgtattccg
     2761 tgtgggtgtc ggagatcatg ctgcagcaga cgcaggtcgg cacggtgatt ccgttctacc
     2821 tgcgcttcat gcggcgcttc ccgacgagtg cggcgctcgc ggcggcgccg ctcgacgacg
     2881 tgctcgcgca ctgggccggg ctcggctact acgcgcgcgc tcgcaatctc tggcgagcgg
     2941 cgcgcgccgt cgcggcgcag cacggcggcg agctgccgac gacgttcgac gcgctgcatg
     3001 cgctgcccgg catcggccgc tcgacggccg gagcgatcct cgctcaggca cttggggcaa
     3061 ggtggccgat cctcgacggc aacgtgaaac gcgtgctcgc gcgctatcac gccgtcgccg
     3121 gttggccggg gttgcccgcg gtcgcgaacg agctctggcg ccacgccgag acgcacacgc
     3181 cgcacgaacg cgtcgccgac tacacgcaag cgatcatgga tctcggcgcg acgctctgcg
     3241 cgcgcacgcg ccccgcctgc accgtttgcc cgctgacgac cggctgcgag gcagcgctcg
     3301 ccggcacgca ggcgctgtat ccggcggcgc ggccgaagcg cgaacggccg caacgccgcg
     3361 tcgccgtgct cgtcgttcgc atgccggacg gccgcgtcct gctcgagcgg cgccccgcgc
     3421 gcggcatctg gggcgggctc tacagcttgc ccgagctgcc ggcggaggac tcgccacgcg
     3481 actggtgcgc gcgcaggctc ggtgcggccg tcgcggccga gcgagcgctc gcgatcatcg
     3541 agcacgcgtt cacgcacttc gatctcgacc tcgagccgcg tttgctcgag ctggccgccg
     3601 agccggctgc gatgatggag cgagacgatt ggctttggtg caggcccggg gcgaagctcg
     3661 acgtcggcgt gccggcgccc gtcgccgccc ttctcagcgg ccctctcgga gcggcatagt
     3721 gggcgcatgg cgagaaaagt gaattgcgta ttgctcggga ccgaggccga gggtctcgac
     3781 cgcgcgccgt atccgggcga gctcggcaag cggatctacg agaatgtgtc gaaggtcgcc
     3841 tggcagcgct ggctcaagca ccagacgatg ctgatcaacg agtacaggct cacgccgatc
     3901 gagccgaagg cgcgcaagtt cctcgaggac gagatgcaga agttcttctt cggccagggc
     3961 tccgaagcgc cgcccgacta caagccgcag taactaaggt ccggttcagc tcaagccgcg
     4021 cgactgggcg acgagccata cgtagccgtc gggatccgtg aacgtgaaat tccgttcgcc
     4081 ccacggttga tcgatgagct tgctcacggg cacgcgcagc ttcgcgaggc gtgcgtgctc
     4141 caaggcggcg tcctcgacct gcagcgcgac cgtcatgccg ccgccgtaga tgcgctgctt
     4201 cggctcgcgc tcgtcgacgt gcagcacgag atagccttcg cccaaggcga ggcccgcgac
     4261 gcgcggctcg tcggtcgaga gcttgcagcc gatcttgtcg cgatagaacg ctttgctgcg
     4321 cgtaagatcc gagacgaacg cgaggatgta gaaaatgccg tacgacttca cggtgccatt
     4381 tgcctcgcgc tagtttccac ggctgcgcac cgcatcctcg tactcgtgct ctttcgcgag
     4441 atattcgagg atcgcggccg cgagctcggg ctggtcggcc gcgaggcggt ggaattcatc
     4501 gtcgagacgc ctatccatgt agggaatgat ggcgagctgc gcttgtgctt cgccggtggc
     4561 catctgcatc gcggcttcca agcaagcgga gtccgatggg ccgcaagcgc tcattcgccg
     4621 agcgacctcc tgacccgcgc gcctcgtcgc attgtcggca taccgcggcc ggcatgcgcg
     4681 gttcttgacg tggctgcccg tgcgcggcgc gctcgtgcaa gcaatgtcga aatcgtcgtc
     4741 gctgttgagc gcgctgaaca cttcccagac gttttcgcgc gccgcctgca cgtcgcggcg
     4801 aaggctgaac agcgtgcgcc tgccttcgac gacgatttcc tcgacgcgct ccgcggcggg
     4861 ggccgccgtc tcggtggtgg ccgcgggctc gctctgagcc gccgcgaagg cgggcgggca
     4921 gagaacgagc agtgccgcgg cgatgggtgc gatcgtgcgc ggcattgctc gattctccgg
     4981 gcgcggctca agtcgccgcg cgattcttga tcaggtcgtc gacgacggcg gggtccgcga
     5041 gcgtcgacgt gtcgccgagc tccttgaaat cgttggccgc gatcttgcga aggatgcgcc
     5101 gcatgatttt gccggagcgc gtcttcggcc ggctcggcgc ccattggatg acgtcgggtt
     5161 gcgcgatcgc gccgatctcc ttgcgcaccc acgcgacgag ctccttgcgc agcgcgtcgc
     5221 tcgagctctc gcccgctttc agcgtcacgt aggcgtagat gccttggccc ttcaagtcgt
     5281 gcgggaagcc gacgaccgcg gcctcggcga ctttcgcgtg gcccacgagc gcgctctcga
     5341 cctcggccgt gcccaagcgg tggcccgaga cgttgatcac gtcgtcgacg cgccccgtga
     5401 tccagtagta gccgtcctcg tcgcggcgcg cgccgtcgcc cgtgaagtac atgcccggga
     5461 aatgcttgaa gtacgtgtcg acgaagcgct ggtgatcgcc gtagaccgtg cgcatctggc
     5521 ccggccagct gtcgagcagc acgagattgc ccgagcacgc gccttcgagg atcttgccgc
     5581 tcggatcgac gatcgccggc ttcacgccga agaacggcag cgtggccgag cccggcttca
     5641 gcttcgtcgc cccgggcagc ggcgtgatca gcatgccgcc cgtttccgtt tgccaccacg
     5701 tgtccacgat cgggcagcgc ccgtcgccga cgacgtggta ataccattcc cacgcctcgg
     5761 gattgatcgg ctcgccgacg gtgccgagca gccgcaagct cgcgcgcgac gtttttttca
     5821 ccgggccgtc accctcgcgc atcagcgcgc gaatcgccgt cggcgccatg tagaggatat
     5881 cgatctggtg cttgtcgacg acttgccaga gccggctcgc gtctgggtac gtcgggatgc
     5941 cttcgtacat gaccgtcgtc gcgccgttcg cgagcgcgcc gtagacgatg tacgtgtgcc
     6001 ccgtgaccca gccgacgtcg gccgcgcacc agaagagctc gccgtcgcgg taatcgaaga
     6061 cgtactggtg cgtgatcgac gcgtgcagca agtagccggc cgtcgtgtgc agcacgcctt
     6121 tcggcttacc cgtcgagccc gacgtataga gaatgaacag cggatcctcg gcgttcatcg
     6181 tctcgggcgg gcagaggtcg gacgcgccgt cttgtacgtc ctcgtaccac ttgtcgcgtc
     6241 ctgaagtcca accgacgttg ccgcccgtgt gcttgacgac gacgacgtgc tcgaccttcg
     6301 ttccgcggac gttgccgagc gcctcgtcga cgttcgcctt caagggaatc ttcttgccgc
     6361 cgcgcacgcc ttcgtcggcc gtgatcacga cgcgcgaggc gcagtcctcg atgcggcctt
     6421 tcaaagcctc gggcgagaag ccgccgaaca cgaccgagtg gatcgcgccg atgcgcgcgc
     6481 aggcgagcat cgcgatcgcg gtctcgggaa tcatcggcat gtagatcgtg acgcggtcgc
     6541 ctttcttgac gccgaggcgc ttcagcgcgt tcgcgagctt gcaaacactc tcgtgcagct
     6601 cgcgatagct gatcagccgc ggcgagccgt tcgggtcgtc gggctcccac aggatcgccg
     6661 tttgctcggg gcgcttcgcg aggtgacgat ccaagcaatt gaccgagacg ttgagctcgc
     6721 cgtcggcgaa ccagcgaacg tgcagatcgt tcgcgtcgaa gct
//
LOCUS       DASSNN010000671         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_491696, whole genome shotgun sequence.
ACCESSION   DASSNN010000671 DASSNN010000000
VERSION     DASSNN010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491696"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VFL84_12520"
     CDS             <1..883
                     /locus_tag="VFL84_12520"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7609496.1"
                     /translation="GDMDMTLDVYRPPAGVTEKRMAIIHLFGGGFFAGNKNAGYIIND
                     AKALGARGYTSVSANYRLQTQAKWPAQIHDVKAAIRWTRANAAKIGVDADKIVVAGYS
                     AGGMLALLAAGTNDNPEFEGTVGTPGVSSKVNACIGVYPLASAQIATGLWPEGQATPD
                     AIKAASPTTYIGPNFAPTIFIHGEADTTVPVSSSIDFFTKLKAANVPTALTVIQGAQH
                     AFDNGALDAVEVMASSIDLFMDRLFINPKPYQGFGGGGGGGRRPGGGGPGGGGGGQGR
                     PGGGGPGGGGAPRPQGN"
     gene            940..1131
                     /locus_tag="VFL84_12525"
     CDS             940..1131
                     /locus_tag="VFL84_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609497.1"
                     /translation="MSRAILAALAGVFLCGAVQAQTYEPIAHWLVLLSGMGVAPNGDL
                     YVTDAYSSDYVHRFDAKGK"
     gene            1212..1358
                     /locus_tag="VFL84_12530"
     CDS             1212..1358
                     /locus_tag="VFL84_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609498.1"
                     /translation="MRIIATDRLNNRIVHLSLDGEFLGVVNGELLLPAARSRSTARTS
                     AAAS"
     gene            1367..1573
                     /locus_tag="VFL84_12535"
     CDS             1367..1573
                     /locus_tag="VFL84_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609499.1"
                     /translation="MTILDKAGKVVTHIGTNTDEGIGTNRVPPEKWRPGYVIAAHGVA
                     TNADGDVFVSEFNTFGRVLTFDRR"
     gene            complement(1584..2498)
                     /locus_tag="VFL84_12540"
     CDS             complement(1584..2498)
                     /locus_tag="VFL84_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET7609500.1"
                     /translation="MHRTPLAALLVAAAALLPSRGAPAQDLEPRLYTNVPLGMNFLGA
                     GYGYSEGNVLFDPAVQLDNAAIEIDGPVLGAGRAVRLGPFSGKVDGAIAHVCLDGSAD
                     FQGERVTRDVCGWTDARARVTVNFIGAPPLRRPQFAGYRQDWVFGASLQLGLPVGDYD
                     PARLVNIGANRVSSKLEVGASKNLTHWLLELSLADTLYEDNTNFYVGRVREQDPIASL
                     QGHAVYRFSTGIWLAIDATRYHGGQTTTGGVTSNDVQSNDRIGITASLPINARQSVKL
                     AASTGVSTRTGTDFDTVAAVWQFAWGGR"
     gene            complement(2588..>3385)
                     /locus_tag="VFL84_12545"
     CDS             complement(2588..>3385)
                     /locus_tag="VFL84_12545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7609501.1"
                     /translation="VLASMAPAAYGQTRADCERAYTPQRAQEGKDVIWEPIEDSMVGR
                     MLEMASVTAADKVYDLGSGDGKIVIAAAKAFAATGVGIEYDAGLVEHSRCLAAAEHVA
                     DRVTFIRGDLFETDFSAATIVTLYLTPRVLARLEPILLAMKPGTRVASYSFSIGDWEP
                     DAEIDSFGDGSAFLFIVPANAAGAWTFRNENGAEAFDVELEQSYQKLAGRAGGSRVIG
                     SLRGAALDFGFIDGAEKVRVEGVVESDRIVATVARGTTSASYVATRN"
BASE COUNT          551 a         1138 c         1170 g          526 t
ORIGIN      
        1 cggcgacatg gatatgacgc tcgacgtcta tcgtccgccg gcgggcgtca ccgagaagcg
       61 catggcgatc attcatttgt tcggcggcgg cttctttgcc ggcaacaaga acgccggcta
      121 catcatcaac gatgccaaag cgctcggcgc ccgcggctac acgagcgtgt cggcgaacta
      181 tcgcttgcag acgcaggcca agtggccggc gcaaatccat gacgtcaagg ccgcgatccg
      241 ctggacgcgc gccaacgccg cgaagatcgg cgtcgacgcc gacaagatcg tcgtcgccgg
      301 ctactcggcg ggcggcatgc tcgcactgct cgcggccggc accaacgaca acccggagtt
      361 cgaaggcacg gtgggcacgc cgggcgtcag ctcgaaggtc aacgcctgca tcggcgtcta
      421 cccgctcgcg agcgcgcaga tcgcgacggg gttgtggccc gagggtcaag cgacgcccga
      481 tgcgatcaaa gcggcatcgc cgacgacgta catcggcccg aatttcgcgc cgacgatctt
      541 catccatggc gaagcggaca cgacggtgcc ggtctcgtcg agcatcgact tcttcacgaa
      601 gctcaaagcg gccaacgtgc cgacggcgct gacggtcatc caaggggcgc agcacgcgtt
      661 cgacaacggc gcgcttgacg cggtcgaagt gatggcctcg tcgatcgacc tgttcatgga
      721 tcggctgttc atcaatccga agccgtatca gggcttcggc ggtggcggcg ggggcggccg
      781 gcgtccgggc ggcggcggcc ccggcggtgg tggcggcggt cagggccggc caggtggtgg
      841 cggccccggt ggcggcggcg cgccgcgtcc gcaggggaac tagcgcaacg caattgcccg
      901 cggcccgccg cgggcccgaa cgagacgccg ggagtgcgta tgtcgagagc aatactcgcc
      961 gcactcgccg gcgttttctt gtgcggcgcc gttcaggcgc aaacctacga gcccatcgcg
     1021 cactggctcg tgctgctgtc cggcatgggc gtcgcgccga acggcgatct ctacgtgacg
     1081 gacgcctact cgagcgacta cgtgcatcgc ttcgacgcga agggcaagta gctcggacgt
     1141 tcggcggcaa ggccgcgccg tacggcttca acatcctgca caagatcgcg atcgatacgc
     1201 gctttgatcc cgtgcgcatc atcgcgacgg atcggctgaa caaccgcatc gtgcatctgt
     1261 cgctcgacgg cgagtttctc ggcgtcgtca acggcgagct gctcttgccg gccgcgcggt
     1321 cgcgatcgac ggcgagaacg tcggcggcgg caagctgaac gggcgcgtga cgatcttgga
     1381 caaagccggc aaagtcgtca cacatatcgg cacgaacacc gatgaaggca tcggcacgaa
     1441 tcgcgtgccg cccgagaagt ggcgccctgg ctatgtgatc gcggcgcacg gcgtcgcgac
     1501 gaatgccgac ggcgacgtgt tcgtctccga gttcaacacg ttcggccgcg tgctgacgtt
     1561 cgatcgccgc tgaccgcggc ggctcagcgc ccgccccacg cgaactgcca cacggccgcg
     1621 acggtgtcga agtccgtgcc cgtgcgcgtc gagacgcccg tgctcgcggc gagcttgacg
     1681 gattgcctcg cattgatcgg caacgaagcc gtgatgccga tgcgatcgtt cgactgcacg
     1741 tcgttgctcg tgacgccgcc cgtcgtcgtc tgcccgccgt gatagcgcgt cgcgtcgatg
     1801 gcgagccaga tccccgttga gaaccggtag accgcatgcc cttgcaatga ggcgatcggg
     1861 tcctgctcgc gcacgcggcc gacgtagaag ttcgtgttgt cctcgtagag cgtgtcggcg
     1921 agcgagagct cgagcagcca gtgcgtgagg tttttcgatg cgccgacctc gagcttcgac
     1981 gagacgcgat tcgcgccgat gttgacgagc cgcgcgggat cgtagtcgcc gacgggcaag
     2041 ccgagttgca agctcgcgcc gaacacccag tcttgccggt agcccgcgaa ctgtggccgt
     2101 ctcagcggcg gcgcgccgat gaagttcacg gtcacgcggg cgcgcgcatc ggtccagccg
     2161 cagacgtcgc gcgtgacgcg ctcgccttgg aagtcggccg agccgtcgag gcagacgtgt
     2221 gcgatcgcgc cgtcgacctt gcccgagaac ggcccgagcc ggacggcgcg gcctgcccca
     2281 agcacggggc cgtcgatctc gatcgccgcg ttgtcgagct gcacggccgg gtcgaacagc
     2341 acgttgccct cggagtagcc gtagccggcg ccgaggaagt tcatgccgag cggcacgttc
     2401 gtgtagaggc gcggctcgag atcctgcgcg ggcgcgccgc gcgagggcag cagcgcggca
     2461 gcggccacca gcagcgcagc gagaggagtc cgatgcattc gcggtcgtcg cttgccgaga
     2521 ctcccgcgaa cgtagcacgg ccgcgcgcgg tgccgcggtg gcccatgggc ctacttgttc
     2581 ggccgggtca gttgcgcgtg gcgacgtagc tcgcggaagt cgtgccgcga gccacggtgg
     2641 cgacgatgcg atcggattcg acgacgcctt cgacgcgaac cttctccgcg ccgtcgatga
     2701 acccgaaatc gagcgcggcg ccgcgcaggc tgccgatgac gcgcgagccg cccgcgcggc
     2761 ccgcgagctt ctggtagctc tgctcaagct cgacgtcgaa cgcctctgcg ccgttctcgt
     2821 tgcggaacgt ccatgcgccg gccgcgtttg ccggcacgat gaacaggaac gcggagccgt
     2881 cgccgaacga gtcgatctcg gcgtccggct cccaatcgcc gatcgagaac gaatacgacg
     2941 cgacgcgcgt gccgggcttc atcgcgagca gtatcggctc gagccgcgcg agcacgcgcg
     3001 gcgtcaggta cagcgtcacg atcgtcgctg cgctgaagtc ggtctcgaag aggtcgccgc
     3061 gaatgaacgt gacgcggtcg gcgacgtgct cggcggccgc gaggcaacgc gaatgctcga
     3121 cgagcccggc gtcgtactcg atgccgacac cggttgcggc gaacgccttt gccgcggcga
     3181 tcacgatctt gccgtcgccg gagcccaagt catagacctt gtcggccgcc gtgacgctcg
     3241 ccatctcgag catgcggccg accatcgaat cctcgatcgg ctcccagatg acgtctttgc
     3301 cttcttgcgc acgctgcggc gtgtacgcgc gctcgcaatc cgcgcgcgtt tggccgtagg
     3361 ccgcgggcgc catcgacgcg agcac
//
LOCUS       DASSNN010000672         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_619117, whole genome shotgun sequence.
ACCESSION   DASSNN010000672 DASSNN010000000
VERSION     DASSNN010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619117"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFL84_12550"
     CDS             <1..373
                     /locus_tag="VFL84_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET7609502.1"
                     /translation="LNLRSGEQLRSKKGPVFDTAIIAGVMAAKRTHELIPFCHPIALE
                     DCKIAIEWGDGNEVVIECSVRATHKTGVEMEALTGATTAALTIYDMCKALTHGIAIRE
                     VVLLAKAGGKRDYTAPGVTTT"
     gene            370..615
                     /locus_tag="VFL84_12555"
     CDS             370..615
                     /locus_tag="VFL84_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET7609503.1"
                     /translation="MTIQVTARYFAAFRERAGRDRELVSTEARTAADLYGELARRHGF
                     ADSMTRCKVAINDELGDWNAALSPGDEVLFFPPVAGG"
     gene            620..1048
                     /locus_tag="VFL84_12560"
     CDS             620..1048
                     /locus_tag="VFL84_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014506880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET7609504.1"
                     /translation="MSFKITSAVIDPAARKRELERSTAGACVTFEGWVRDHNDGEAVQ
                     ALEYEAHEAIAEKEGARIVAEALEKFAVTAVHCEHRVGKLAIGDCAVWVGVSADHRGA
                     AFDACRYVIDETKARVPIWKKEHYASGATGWVNCATRAAG"
     gene            1171..2517
                     /locus_tag="VFL84_12565"
     CDS             1171..2517
                     /locus_tag="VFL84_12565"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET7609505.1"
                     /translation="MRIAGFLALFVSTSCSAATYYVSNDGNDRASGKSAAEAWATLDK
                     VNRQSFKTGDVVLFRDGDRWQGKLAVDWSGTASAYATVGAYHLENGTAVKGSRERRPI
                     IEGAGRFPGGGIYDALIMVNTRDYVRIEDIEVRNSEGRGIGFARANFGDVVNVVVDGA
                     YVDGIHFLKSDHGSVSRSFVTHAGLVFPRDGRKHAWAAAITFVGSDSGRVVESTVAET
                     YGEGINTNHGSTGTLIENNRVFAVRAVGIYADAAPKTTIRGNIVVGTAKREFWRGGQS
                     VGAGIAINDEKYNYKAGGGSLPDDVQSKDAKIEGNVVVYTSTGIALWSAVPQASHDNL
                     IVAGNTLIDNDRQISGLGTKAPGGLLADNVLLSLSKGTSDVDSAKLSGLTARNNYFSQ
                     GDPGGDLGHAGNRREGIAVRETGWRSIDSIDDVDWDELARLARSSRANEARAADQN"
     gene            complement(2508..>2764)
                     /locus_tag="VFL84_12570"
     CDS             complement(2508..>2764)
                     /locus_tag="VFL84_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609506.1"
                     /translation="FQPADRFSIVPKLGMSHWRLEQDLTGFFLPPGLVDSSESGNDWI
                     GRVSFEWHAGQRLRLYAAYTEAHYDFGDSTAPSFGFKFQF"
BASE COUNT          507 a          903 c          944 g          410 t
ORIGIN      
        1 gctcaacctg cgttccggcg aacagctgcg ctcgaaaaag ggccccgtgt tcgacacggc
       61 gatcattgcc ggcgtcatgg ccgcgaagcg cacgcacgag ctgatccctt tttgccaccc
      121 catcgcgctc gaggactgca agattgcgat cgagtggggc gacggcaacg aggtcgtcat
      181 cgagtgctcg gtgcgcgcga cgcacaagac cggcgtcgag atggaggcgc tgaccggagc
      241 gacgaccgcc gcgctgacca tctacgacat gtgcaaggcg ctgacccacg ggatcgcgat
      301 ccgcgaagtc gtgctgctcg cgaaagccgg cggcaagcgc gattacaccg ctccgggtgt
      361 gacgacgaca tgacgattca ggtcacggcc cggtacttcg cggcgttccg cgagcgcgcg
      421 gggcgcgacc gcgagctcgt gagcacggaa gcgcgcacgg ccgcggatct ctacggcgag
      481 ctcgcgcgcc ggcacggctt cgccgattcg atgacgcgct gcaaggtcgc gatcaacgac
      541 gagctcggcg actggaacgc ggcgctgtcg cccggcgacg aggtgctgtt cttcccgccc
      601 gtcgccggcg gctagcctca tgtcgttcaa gatcacatct gccgtcatcg atccggccgc
      661 gcgcaagcgc gagctcgaaa gaagcacggc cggcgcctgc gtgacgttcg aaggctgggt
      721 gcgcgatcac aacgacggcg aggccgtgca ggccctcgag tacgaggcgc acgaggcgat
      781 tgccgagaag gaaggcgcgc gcatcgtcgc ggaggcgctc gagaagttcg ccgtgacggc
      841 cgtgcactgc gagcatcgcg tcggcaagct cgcgattggc gactgtgcgg tgtgggtcgg
      901 cgtcagcgcc gatcatcgcg gcgccgcctt cgacgcgtgc cgctacgtca tcgacgagac
      961 gaaagcgcgc gtgccgatct ggaagaaaga gcactacgcg agcggcgcga cgggctgggt
     1021 caactgcgcg acgcgcgccg ccggctaaag accccgcgca ctatcgagag ctatccggcg
     1081 cctgcgccgg tccgtgcgga caacgcccgg ctggtacacc tcttgcacgc tcagcgatga
     1141 actttcccgc ggcggagggg tgtcatcgtg atgcggattg cgggattcct ggccctgttc
     1201 gtctcgacga gctgctcggc ggcgacgtac tacgtgagca acgacggcaa cgaccgcgcg
     1261 agcggcaagt ccgctgcaga agcctgggcg acgctcgaca aggtcaaccg gcagtcgttc
     1321 aagaccggcg acgtcgtcct gtttcgcgac ggtgaccgct ggcaaggcaa gctcgccgtc
     1381 gactggtccg gcacggcatc cgcgtatgcc accgtcggcg cctaccacct cgagaacgga
     1441 acggcggtga agggatcccg cgagcggcgc ccgatcatcg aaggcgccgg ccgctttccc
     1501 ggcggcggca tctacgacgc gctgatcatg gtcaacacgc gtgactacgt tcgcatcgag
     1561 gatatcgagg tacggaattc ggaagggcgc ggtatcggct tcgcgcgcgc caacttcggc
     1621 gacgtcgtga acgtggtcgt cgacggcgca tacgtcgacg gcattcattt cctgaagtcg
     1681 gaccatggct ccgtctcgcg ctcgttcgtg acgcacgccg gtctcgtctt tccgcgagac
     1741 ggccgtaagc acgcgtgggc cgcggcgatc accttcgtcg gctccgattc gggccgcgtc
     1801 gtcgagtcga cggtggccga aacctacggc gaaggcatca acacgaatca cggctcgacg
     1861 ggaacgctga tcgagaacaa ccgcgtgttc gcggtgcggg ccgtcggcat ctacgccgat
     1921 gcggcaccga agacgacgat ccgcggcaac atcgtcgtcg gcacggcgaa acgcgagttc
     1981 tggcgcggcg gccagtcggt gggcgccggc atcgcgatca acgacgagaa gtacaactac
     2041 aaagccggcg gcggctcgct gcccgacgac gtccagtcga aggacgcgaa gatcgagggc
     2101 aacgtcgtgg tctatacgtc gaccggcatc gcgttgtggt cggccgtgcc gcaggcgtcc
     2161 cacgacaact tgatcgtcgc gggcaacacg ctgatcgaca acgaccggca aatctcgggc
     2221 ctcggcacga aggcccccgg cggcctgctc gccgacaacg tgctgctcag cctgagcaag
     2281 ggcaccagcg acgtcgactc ggcgaagctt tccgggctca cggcccgaaa caactatttc
     2341 agccaggggg atcccggagg cgatctcggc catgccggca accggcgcga aggcatcgcc
     2401 gtgcgtgaga cgggttggcg cagcatcgac tcgatcgacg acgtcgactg ggacgagctc
     2461 gcgcgcctcg cgcgctcgtc gagagccaac gaggcccgtg ccgccgatca gaactgaaac
     2521 ttgaaaccga agctcggggc cgtcgagtcg ccgaagtcgt agtgggcctc cgtgtaggcc
     2581 gcataaaggc gcaatcgctg gccggcatgc cactcgaagc tcacgcggcc gatccaatcg
     2641 ttgccggact cggacgagtc gacgagcccg gggggcaaaa agaagccggt cagatcttgc
     2701 tcgagacgcc aatgactcat gccgagcttc ggaacgatgc tgaaccggtc cgccggttgg
     2761 aacg
//
LOCUS       DASSNN010000673         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_114640, whole genome shotgun sequence.
ACCESSION   DASSNN010000673 DASSNN010000000
VERSION     DASSNN010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114640"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..547)
                     /locus_tag="VFL84_12575"
     CDS             complement(125..547)
                     /locus_tag="VFL84_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET7609507.1"
                     /translation="MGVLRYLVFIAVGLVVASVLFLGLSFLVSANFDSSVAYVERWIV
                     RVTCDQYELAGTVRDTRGRPVPYAVVEVSFLGERLTTRSNVDGTFKLAADETVCDRRP
                     PRSVQLLVVAEDFRPKTTAVPYDAGSIDVTLDARDFRP"
     gene            complement(548..1588)
                     /locus_tag="VFL84_12580"
     CDS             complement(548..1588)
                     /locus_tag="VFL84_12580"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7609508.1"
                     /translation="MRAAAGAALAFGAFGAYAQPAAPKRIALGGRRVSVIDIHAHCLF
                     PEVASRVPNVRVPRAAPPTLVLGPERIAAMDERGIDIQALSVNTYWWYSASRDDARTV
                     VAMNDDGIAAWCKQHSSRFVGLCSVALQFPDLAAEQLEHAVKNLDLRGASIGGHVNNE
                     PPTSEKYDPFWAKAEELGVPVFMHPNNALNVAREGALDGRGGLGNIVGNPLETTVFLS
                     RMIFDGTLDRFPRLKLCAAHGGGYMTSYLGRSDAACIVRDDANCANKRKPSEYFKDQI
                     VVDSMVFSSEGLRHLVAETGVTQVVYGSDQPYFWPDTIDIIVEAPFLSDADKEAILSG
                     NLRRLLRISGRG"
     gene            1819..>2750
                     /locus_tag="VFL84_12585"
     CDS             1819..>2750
                     /locus_tag="VFL84_12585"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7609509.1"
                     /translation="MKATVCIRAAAGLIGAATLTVSTSTWAQPPGPAGEAFKVVIVAE
                     GLKNPWSMAWLPNGDMLVTERGTGDYPGSLRIIRGGKLLPTPVPGVPTVRAQGQGGMQ
                     EVAVHPNFAQNHFIYLSYAKPRNGTAQTCRTPAPANLPAGQPPCIEGTTALMRAKLEG
                     DKLVEAKEIFEAKAWNDAPGHFGARITFDGQGHLFMSVGDRMAGLFPRTPSGAMDPNL
                     EGHPAQNLGLHQGKIIRLNDDGSVPQDNPFVGRAGALPEIWSYGHRNPQGLYFDRATG
                     TLWESEHGPQGGDELNVIEKGKNYGWPVIGYGAN"
BASE COUNT          494 a          930 c          899 g          427 t
ORIGIN      
        1 cagcgcaacg gctcgtcgct gccttcgtcg tcgtcttgag cggcggcgct cgcgagcgac
       61 aaagcagcga cgcttccgag caacgcggaa agccttgtgc gtgtcatggc gaacctccag
      121 ctcgtcaggg cctgaagtcg cgggcgtcga gcgtcacgtc gatggaaccc gcatcatagg
      181 gcacggccgt cgtcttcggt cgaaaatctt cggcaacgac tagcagctgc acgctgcgcg
      241 gcggccggcg atcgcagacg gtctcgtcgg cggctagctt gaacgtgccg tcgacgttgc
      301 tgcgcgtcgt gagccgctcg ccgagaaacg aaacctcgac gacggcgtac ggcaccggcc
      361 gcccgcgcgt gtcgcgcacg gtgccggcga gctcgtactg gtcgcaagtc acgcgcacga
      421 tccagcgctc cacgtacgcg acgctcgagt cgaagttcgc gctgacgagg aagctcaggc
      481 cgagaaacag caccgacgcg acgacgagcc cgacggcaat gaacaccagg taccgcagga
      541 cccccatcta ccctcggccc gagatacgca gcaaccgtcg caagttgccg ctcaagatcg
      601 cttccttgtc cgcgtccgac aggaacggcg cttcgacgat gatgtcgatc gtgtcgggcc
      661 agaagtacgg ctggtcggag ccgtagacga cttgcgttac gccggtttcg gcgacgagat
      721 ggcgcaagcc ttccgaggaa aacaccatcg agtcgacgac gatttggtcc ttgaagtact
      781 cgctcggctt gcgcttgttc gcgcagttcg cgtcgtcgcg gacgatgcac gccgcgtcgc
      841 tgcgacccaa gtacgacgtc atgtagccgc cgccgtgtgc ggcgcagagc ttcaaccgcg
      901 gaaaccggtc gagcgtgccg tcgaagatca tgcggctcaa gaacaccgtc gtctcgagcg
      961 gattgccgac gatgttgccc aagccgccgc ggccgtcgag cgcgccctcg cgcgcgacgt
     1021 tgagcgcgtt gttcgggtgc atgaacacgg gcacgccgag ctcttccgct ttggcccaga
     1081 acggatcgta cttctcgctc gtcggcggct cgttattgac gtggccgccg atcgacgcgc
     1141 cgcgcagatc gaggttcttg acggcgtgct cgagctgctc ggccgcgaga tccggaaact
     1201 gcagcgcgac cgagcagaga cccacgaacc gcgacgagtg ctgcttgcac caggcggcga
     1261 tgccgtcgtc attcatcgcg acgaccgtgc gcgcgtcatc ccggctcgcc gaataccacc
     1321 aatacgtgtt gacgctgagc gcctggatgt cgatgccgcg ctcgtccatc gccgcgatgc
     1381 gctcgggccc gagcacgagc gtcggcggcg ccgcgcgcgg gaccctgaca ttcggcacgc
     1441 ggctcgcgac ttccgggaac aggcagtgcg catggatgtc gatgaccgag acgcgcctgc
     1501 ctccgagcgc gatgcgcttc ggcgccgccg gctgtgcgta cgcaccgaac gcgccgaatg
     1561 caagcgccgc gccggccgcg gccctcatga atgctctgcg atcgctcata gatgcccccg
     1621 gagcccaagc ctcgcccaat ggcctcgaag cgtcaatcgg gcccgaatgc agatttctcg
     1681 atggctttcg gtcgcttccg gccgagccgt cgggctaaga taatcgccga cgcccgttcg
     1741 ggcagataag aacgatcgca gcgcggtagc gctggttttg gcaacgaagc gccgggggcg
     1801 ctcaacaaag gaacatccat gaaggcaaca gtatgcattc gcgcggctgc cgggctcatc
     1861 ggtgccgcga cactgacggt ttcgacgtcg acgtgggcgc agccgcccgg cccggccggc
     1921 gaagccttca aagtcgtgat tgtcgccgaa gggttgaaga atccctggag catggcgtgg
     1981 ctgccgaacg gcgacatgct cgtcacggag cgcggcaccg gcgactaccc cggctcgctg
     2041 cgcatcattc gcggcggcaa gctgctgccg acgccggtgc ccggcgtgcc gaccgtgcgc
     2101 gcgcaaggtc aaggcggcat gcaggaagtc gccgtgcacc cgaacttcgc gcagaaccat
     2161 ttcatctact tgagctacgc gaagccgcgt aacggcaccg cgcaaacgtg ccgaacgcca
     2221 gccccggcga atttgcccgc cggccagccg ccctgcatcg agggcacgac cgcgctgatg
     2281 cgcgccaagc tcgagggcga caagctcgtc gaggccaaag agatcttcga ggcgaaggcc
     2341 tggaacgacg cgccgggtca cttcggcgcg cgcatcacgt tcgacggcca aggtcatctc
     2401 ttcatgtcgg tcggcgaccg catggcgggc ctgttcccgc gcacgccgtc gggcgcgatg
     2461 gatccgaacc tcgagggcca tcccgcgcag aacttgggtc tccatcaagg caagatcatc
     2521 cggttgaacg acgacggctc cgtcccgcaa gacaatccgt tcgtcggtcg cgccggcgcg
     2581 ctgcccgaga tctggagcta cggccaccgc aatccgcagg gcctgtactt cgatcgcgcc
     2641 acgggcacgc tctgggagag cgagcacggc ccgcaaggcg gcgacgagct caacgtcatc
     2701 gagaagggca agaactacgg ctggcccgtc atcggttacg gcgcgaacta
//
LOCUS       DASSNN010000674         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_244708, whole genome shotgun sequence.
ACCESSION   DASSNN010000674 DASSNN010000000
VERSION     DASSNN010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244708"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFL84_12590"
     CDS             complement(<1..255)
                     /locus_tag="VFL84_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET7609510.1"
                     /translation="MLADRVRHEAFRAALKKAIEPGKSAVADIGAGLGVLAFFARELG
                     AREVWLYDPGPALALAEAVADRNGIDGLHFVAERSLDVADP"
     gene            complement(292..1131)
                     /locus_tag="VFL84_12595"
     CDS             complement(292..1131)
                     /locus_tag="VFL84_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET7609511.1"
                     /translation="MSVRPDKRKERLYRRGLVALLAGLMCVGVALADPMKWREYPGVE
                     YYGFELPTDFRRPAEWVFARLMYPSIGWGGSWKNGGTSWTIDYPRGDRHIAALVRRLS
                     VIDARSVEQPVDLDDGDDVFYWPWLYAVEVGQWDLTDAQAAKLREYLLRGGFLMVDDF
                     HGTVEWGTFVDSMRRVFPDRPIVDIPNDDPIFHAFWDLEERQQIPGYQFVFSGRRFEQ
                     DGYEAHWRGIYDDEGRVMVVINHNMDLGDAVEHSDTPAYPEELSAQAMRTFLNYIVYA
                     MTH"
     gene            1223..2611
                     /locus_tag="VFL84_12600"
     CDS             1223..2611
                     /locus_tag="VFL84_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET7609512.1"
                     /translation="MKDLTQGSIVRNILAMAAPLAAGMIFQTLYFLVDLYFVTRLGPT
                     AVAGVGAGGTLTFAIMALTQVLGVGAVALIAQAVGRRDQAEANLVFNQSVSLAVVCAL
                     ATLIGGNAFAGLYLSLVGADAATQSLGATYLYWFLPGLALGFAQVVMGSGLRGTGIVK
                     PTMVIQALSVLLNIVLAPILIAGWGTGYAMGVAGAGFASSIAIVVGIVLLAWYFLRRE
                     KYVGFDARLWRPQLKVWKRMLVVGLPAGAEFGLMFCYMVVVYWVISDFGATAQAGFVI
                     GSRIMQSIFLPAMAIAFAAGPIAGQNYGAHQTARVRETFVKAAGLSTVVMICVTAFLQ
                     WRPEMLVAFFPSEPEEQAVGATFLRIISWTFVAQGIVFTCSGIFQGLGNTIPAMLSSA
                     IRLCVFVPLAVWVASRPAFHLDEVWWLSVSTVWLQAAVSYVLLRHQFKRRLVDRPAPA
                     VPPAAAPAPAES"
BASE COUNT          397 a          866 c          883 g          494 t
ORIGIN      
        1 cgggtcggcg acgtcgagcg agcgctcggc aacgaaatgg aggccgtcga tgccgttgcg
       61 gtcggcgacg gcctcggcca gcgcgagcgc gggcccgggg tcgtagagcc acacctcgcg
      121 cgcgccgagc tcgcgggcga agaacgctag cactcccaag ccggcgccga tgtcggcgac
      181 ggcgctctta ccgggctcga tcgctttttt cagggccgcg cgaaacgcct cgtggcgaac
      241 gcgatccgcc agcatgcgtc gatggaattc gatcgacatg cgggaggcag gctaatgcgt
      301 catcgcgtag acgatgtagt tcaagaaggt gcgcatcgcc tgggccgata gttcctcggg
      361 atacgcgggc gtatcggagt gctcgacggc gtcgcccaag tccatgttgt gattgatgac
      421 gaccatgacg cggccttcgt catcgtagat gccgcgccaa tgcgcttcgt agccgtcctg
      481 ctcgaaacgc ctaccgctga acacgaactg gtaaccgggg atttgctgcc gttcctcgag
      541 atcccagaac gcgtgaaaga tcggatcgtc gttcggaatg tcgacgatcg gccggtccgg
      601 gaacacgcgc cgcatgctgt cgacgaacgt tccccattcc accgtgccgt gaaagtcgtc
      661 gaccatcagg aagccgccgc gcagcaagta ctcgcgcagc ttcgccgctt gcgcgtcggt
      721 gaggtcccat tgacctacct cgactgcgta gagccagggc caatagaaca cgtcgtcgcc
      781 gtcgtcgaga tccacgggct gctcgacgga gcgggcatcg atgacggaca accgccgcac
      841 gagcgcggcg atgtgccggt cgccgcgcgg gtagtcgatc gtccagctcg tgccgccgtt
      901 cttccagctg ccgccccagc cgatcgacgg gtacatgagg cgcgcgaaga cccactccgc
      961 cggcctgcgg aaatccgtcg gcagctcgaa gccgtagtac tcgacgccgg ggtactcgcg
     1021 ccatttcatg gggtccgcga gcgcgacgcc cacgcacatg aggcccgcga gcagcgcgac
     1081 gaggccgcgg cgatagaggc gttccttgcg tttgtcgggc cgtacgctca cccctccgag
     1141 catagcgaac taggccgcga ccgcactaga gcagtttcac ggtagagcga gtattctgtg
     1201 cggccatttc gcgaggttgc ccatgaagga cctgacccag ggctcgatcg tccgcaacat
     1261 tctcgcgatg gccgccccgc tcgcggcggg catgatcttc caaacgctgt acttcctcgt
     1321 cgacctctat ttcgtcacgc ggctcggccc gacggcggtc gcgggtgtcg gcgccggcgg
     1381 cacgctgacg ttcgcgatca tggcgttgac tcaagtgctc ggcgtcggcg cggtcgcgct
     1441 gatcgcgcaa gccgtgggcc gaagagacca ggccgaggcg aatctcgtgt tcaaccagtc
     1501 ggtgtcgtta gcggtcgtgt gtgcgctcgc gacgctgatc ggcggaaacg cgttcgcagg
     1561 tttgtacctc tcgttggttg gagccgacgc cgcgacgcag agcctcggcg ctacgtatct
     1621 ctattggttc ctgccgggct tggcgctcgg cttcgcgcag gtcgtcatgg ggtcggggct
     1681 gcgcggcacc ggaatcgtca agccgacgat ggtcattcaa gccctgtccg tgctgctgaa
     1741 catcgtgctc gcgccgattc tgatcgcggg ctggggaacg ggctatgcga tgggcgtcgc
     1801 gggcgccggg ttcgcgagct cgatcgcgat cgtcgtcggc atcgtgctgc tcgcctggta
     1861 tttcctgagg cgcgagaagt acgtcggttt cgacgcgcgg ctctggcggc cgcagctcaa
     1921 agtctggaag cgcatgctcg tcgtcggctt gccggccggc gccgagttcg ggctcatgtt
     1981 ctgctacatg gtcgtcgtct actgggtcat cagcgacttc ggcgcaaccg cgcaggccgg
     2041 tttcgtcatc ggctcgcgca tcatgcaatc catcttcctg cccgcgatgg cgatcgcatt
     2101 cgctgcgggc ccgatcgcgg gccagaacta cggcgcgcac cagaccgcgc gcgtccgcga
     2161 gacgttcgtg aaggccgccg ggctcagcac ggtcgtcatg atctgcgtga ccgcgttctt
     2221 gcagtggcgg ccggagatgc tcgtcgcgtt tttcccctcg gaacccgagg agcaggcggt
     2281 cggcgcgacg ttcctgcgca tcatctcctg gacgttcgtc gctcagggca ttgtgttcac
     2341 gtgctccggc atcttccagg gcctcggtaa cacgattccc gcgatgttga gctccgcgat
     2401 tcgcctctgc gtgttcgtgc cgctcgccgt atgggtagcg tcgcggcccg cgttccacct
     2461 cgacgaagtc tggtggctgt cggtctcgac ggtctggctg caagccgcgg tcagctacgt
     2521 cttgctgcgc caccagttca aacgccggct cgtcgatcgg cccgcaccgg ccgtgccgcc
     2581 cgcggccgcg ccggcgccgg ccgaaagctg actcgcgggt cggcatttcg gggtgcgcct
//
LOCUS       DASSNN010000675         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1863362, whole genome shotgun sequence.
ACCESSION   DASSNN010000675 DASSNN010000000
VERSION     DASSNN010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863362"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFL84_12605"
     CDS             <1..747
                     /locus_tag="VFL84_12605"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7609513.1"
                     /translation="NGRAPRLSPALYGAVIDAAHRRALRVNAHVFYHSDAEALVAAGI
                     DGLVHLVRDAEMSDELIGAIVARNVYVNANMSNPRRSTQAGLPQWLTPADPLFALLNE
                     SVAPELLAKIQAAFAQRDPRAAADARERYAILERSLRKLDAAGAKVVLGADTGVEDHP
                     FGVAEHLELEAMVDAGLSPAHALAAATSRAAEYLHLADRGSLVPGKRADLLVLDASPL
                     DDIVNTRRISQVFIDGTEIDRAALRSQLSE"
     gene            complement(821..1039)
                     /locus_tag="VFL84_12610"
     CDS             complement(821..1039)
                     /locus_tag="VFL84_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609514.1"
                     /translation="MSPADQNELTVLTNERERCHRNWVDGLSTVATNGRIHPTGRQYE
                     GLERLLRSLRISQQRLETFERGLDRART"
     gene            complement(1442..>2038)
                     /locus_tag="VFL84_12615"
     CDS             complement(1442..>2038)
                     /locus_tag="VFL84_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609515.1"
                     /translation="YICAACGIATFALGQGRENRIYKAAVIALTQDAALRADFERQFV
                     AKAREHNYDAVVSYDVAPNVTDVRDKEFVDTMLANKVDIVLLVRPAAIGPGASLDSVR
                     KEVSAKTLTDMRAFAKKVSDSNGNDLIAVVHQAVYLFYGRDPEVISSGAVWLDEPVQD
                     RVEAVARLQDLMLENLDAARPAIRRFYRMNPEAPAARQ"
BASE COUNT          330 a          678 c          652 g          378 t
ORIGIN      
        1 aacgggcgcg cgccgcgttt gagccctgcg ctgtacggcg ccgtcatcga cgcggcccac
       61 cgacgggctt tgagggtcaa cgcgcacgtg ttctatcact cggacgccga agcgctcgtc
      121 gcggcgggta tcgacggcct cgtgcacttg gttcgcgacg ccgagatgag cgatgaattg
      181 atcggcgcga tcgtcgcgcg caacgtctac gtcaacgcga acatgtcgaa cccgcgccgt
      241 tcgacgcagg cgggtcttcc gcaatggctg acgcccgccg atccgctgtt cgctctgctg
      301 aacgagtccg tcgcccccga gctgctcgcg aagatccagg cggcgttcgc gcagcgcgat
      361 ccgcgagcgg cagccgacgc gcgcgagcgc tacgcgatcc tcgagcgcag cctgcgcaag
      421 ctcgatgcgg ccggcgcgaa agtcgtgctc ggcgccgata ccggcgtcga ggatcatccg
      481 ttcggcgttg ccgagcacct cgagctcgag gcgatggtcg acgcgggtct atcgcccgcg
      541 cacgcgctcg ccgccgcgac gagccgcgcc gcggaatatc tgcatctcgc cgatcgcggc
      601 agtctcgtgc ccggcaagcg cgccgatctg ctcgtgctcg acgcgagccc gctcgacgac
      661 attgtcaaca ctcgtcgaat ctcacaagtg ttcatcgacg gcaccgagat cgatcgggcc
      721 gctttgcgct cgcaactatc agaatgagcc gcgcgccgga gctcgcggtg cgcgaccgcg
      781 aactcctcac cccgaggttg gagtccgcgc caatatcgcc tcaagtccgc gcgcggtcga
      841 gccctcgttc gaaggtttcc aggcgttgct gggagattct gagcgagcga agcaagcgct
      901 cgagaccttc gtactggcgg cctgtcggat gaatccggcc gttcgtggct acggtgctga
      961 gcccatcgac ccagttgcgg tggcagcgct cgcgttcgtt cgtcagcacg gtcaattcgt
     1021 tctggtccgc tgggctcatg ctcgagctcc cttggagaac gcgcgacccg aaccgtcgca
     1081 agcgacggcg agaatcagcg gggtggatga tctagaccga gtgtgcgctc aatcgacgcc
     1141 gattgcactg ccgattttgg cagctaagcc gccgtttccg cggcgcggcg cagagcgtcc
     1201 ggaggcgtct cgtccgccaa actcagacgc atgcgcctgg aatgcttgag gtgaagccgc
     1261 aacgcttcct ccatctcggc caggagctcc ttggcgagcg ctacatcgaa tccctttcgc
     1321 tcacgcctcg ctatcgtctc gcgttgcaga gctacgagcc gctcaccctc gcgaaggtgt
     1381 atgtccgctt gcgcgagcgc ttgctccaac atttcacgct cggtcgtgtg catgggcgat
     1441 ctcactggcg cgccgccggc gcctcgggat tcattctgta gaagcggcgg atggccggtc
     1501 gagccgcgtc gaggttctcg agcatcaagt cttgcagccg cgcgacggct tccactctgt
     1561 cctgaacggg ctcgtcgagc cacacggcgc ccgaagaaat cacctccggg tcgcggccat
     1621 agaagagata caccgcctga tggacgaccg caatcaaatc gttgccgttc gaatcgctga
     1681 ccttcttcgc gaacgcgcgc atgtcggtca gagtcttggc gctgacttcc ttgcgcacgg
     1741 agtcgagcga agcgccgggc ccgatggcag ccggccgaac cagcagcacg atgtctactt
     1801 tgttggcgag catcgtgtcg acgaactctt tgtcgcggac gtccgtgacg ttcggcgcga
     1861 cgtcgtagct gacgaccgcg tcgtagttgt gctcgcgagc tttggcgacg aattggcgct
     1921 cgaagtccgc gcgcagcgcc gcgtcctgcg tgagcgcgat caccgcggct ttgtagatgc
     1981 ggttttctct tccttggccg agcgcgaaag tcgcgatgcc gcatgcggca cagatata
//
LOCUS       DASSNN010000676         7600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1246580, whole genome shotgun sequence.
ACCESSION   DASSNN010000676 DASSNN010000000
VERSION     DASSNN010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246580"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..351)
                     /locus_tag="VFL84_12620"
     CDS             complement(1..351)
                     /locus_tag="VFL84_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609516.1"
                     /translation="MQLHWFKAPGGAWPAFDAVDPEQIDQYGVFVVWRNGNIARVSAV
                     LYVGRGSMRQELARCQRDPVFRDARDLYVTWAQVSDVEKIDSVAAYLYQKLRPLWGEI
                     VHVPPLAVNTPWAA"
     gene            662..889
                     /locus_tag="VFL84_12625"
     CDS             662..889
                     /locus_tag="VFL84_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609517.1"
                     /translation="MKPDAVFRLVTEMSDLQKLSLLQLLKIGATSPEVRSVVLECRDR
                     GLDVDGTLAEVLRRFADKRMPAEPTGRVTHH"
     gene            1005..1868
                     /locus_tag="VFL84_12630"
     CDS             1005..1868
                     /locus_tag="VFL84_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7609518.1"
                     /translation="MNRPMSVLLVLAALLSSASVTAQGTLDRIRERGEIRIGYRGDAR
                     PLSFEENGAPAGYSVDLCKRVAAGIGEQLKMPAMKATYVKVTTDNRFDALVKGDIDIE
                     CGATTITLGRLERVDFTLMTFVTGGTFLSKAENRVGGPADLAGKRIAVPRGTSTATAL
                     QAYLKENSIDARVVTVDDRGAGMSRLTRGDVDALAGDQIVLLGDALDVLETNKDANFS
                     FADALFSYEPYGFMVRRNDADFRLAVNRELAKVFRDGDQAQIYQNWVGSNGVKPSPML
                     IAMYLVQGLSE"
     gene            complement(1888..2208)
                     /gene="sugE"
                     /locus_tag="VFL84_12635"
     CDS             complement(1888..2208)
                     /gene="sugE"
                     /locus_tag="VFL84_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HET7609519.1"
                     /translation="MAWILLVVAGVFEVVWAYYMKQSSGFTRVLPSVITIATMIVSFV
                     LLSLAMRTLPLGTAYTVWTGIGAVGTFAVGVAVLGEPADAMRVAAAALIVCGLILMRL
                     SSAQ"
     gene            2437..2841
                     /locus_tag="VFL84_12640"
     CDS             2437..2841
                     /locus_tag="VFL84_12640"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609520.1"
                     /translation="MLKRTLGSLLVGGALLAAGSLQAHHSLAGVYDIRGQGSVSGVLT
                     KVAFTNPHGAMYLDVTNADGTVTNWVMTTGSANTLANLGFGTGGPNTVKAGDAVTITY
                     FPARNGKPLGFIRSIVLADKREIQISSGSSSD"
     gene            3110..>7600
                     /locus_tag="VFL84_12645"
     CDS             3110..>7600
                     /locus_tag="VFL84_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7609521.1"
                     /translation="MTLEALKLPGYQHGERIHDSSAAVVYRARRVSDGARVVIKRSQG
                     HSVSVRQLTRYRNEFELLRSLDCRGVVKAHDLLRHDGHVALVLEDLPGTSLRAWIQSS
                     PEAPIGERLAIAIELAEIVANVHAANVIHKDVSSHNVVYDAQKGRCTLIDFGIATRLR
                     SEESEFKAAGALEGTLAYIAPEQTGRMNRSLDHRADLYSLGVTLYELFTGELPHESAD
                     QLEMVHFHIAVKPVPPRERHARVPEALSEIVLKLLQKEPEYRYQSAAGLVADLRRCLA
                     AVDAGRPAERFELGSADVVDRFDPPQKLYGRAAETSALLASFERVARGGVETVTVAGQ
                     PGIGKTSLVQEIYQPITKRRGYFVAGKFDLLQQNVPFSALVAALQDLVQQLLMEGEEE
                     IAAWREAIDAAVHPNGQLIVDVVPALELIIGPQPRVAELEGLEAQNRFHLAFQNFVQL
                     FCKKSHPLVLFLDDMQWADSASLNLITRLVSARATESLLLVLTYRDAEVEANHPFLLA
                     VKEQRKQGVPVLAIELKPLGVPEVAEFVADTLHQDARTAMPLAEVIQQKTGGNPFFMR
                     QFLRALYGARLITFDGAAKRFRYDVAAVKSAAITENVAELLATELQRLPASTRETLRV
                     AAVIGGRFELRLLAKVRKQSPAATNESLRPAVEAGLVVPLSGLESVDANALQSPLVYG
                     RFGFRHDRVQQAAYATLPESARPQLHLEIGRAWLAMASSAELEARLFDVVGHLNEGRG
                     GIVNAEERRRLADLNRRAGIKARDATAYDLAVSSFRTAIELDGAPAWRDRYSVQFDTH
                     RRLAEALGLTADSAGALAVIEHALEHAEPLIDRTQLCAIKTNVLLIMGRIPEALACGR
                     AAAREFGVDLPEQPEEVRALLQREIGSIRQRAAAVGIEKWLDLPAMEDPSQIALMGLL
                     MHCLPAAFQTDQESYALLCCTMVRLSIEHGNCPLSARAYGSFAALLSSQLSEYEDAYR
                     FAKLGVDLAHKLGETSVLSGVYFLWAMFASHWVKPVDESIDLYEQAVRFGLQSGDHVH
                     AGYSVARRFSHLQVRGAPLAELREEGRAALETLHRISDAANREFLQPRVELIDWLRGE
                     RPHGNTLGTAELDESARTAEIQKRGNRSFEADWFMVLTILRYHAEEFEAAHAYAKIAA
                     GLQPFCAGFVTRAEHALYYALATAAVYPAAAAGERAAHDATLAAIRKEMARWVRLCAA
                     NVEHMQLLVEAECARLRDARIEAADYYDRSIAAARGNGYLGIEALAAELAGRFWLAAG
                     KPDFARIYLERALDAYVAWGAAGKAADLSSKLGLVPAGSAAASAASASTTLGSQKRSD
                     ALDLATLLKASQAIAGEIVLERLLVELMDIMRENAGAESVVIVLESNGEFLVQAVKTA
                     SGVARVLVAEPLRSSVACSPGIVNYVLRTSELVVLDDAALHGKYRGDAYVASRRPKSI
                     LCAPVAHKGKLIGALYLENNQVAGAFTPDRLEALNILMSQVAVSIDNATLYSRQEQQS
                     RTI"
BASE COUNT         1256 a         2582 c         2546 g         1216 t
ORIGIN      
        1 ctacgccgcc cagggcgtat tgaccgcgag cggcggcacg tgaacgatct cgccccagag
       61 cggccgcagc ttctgataga ggtaggcggc caccgagtcg atcttctcga cgtcgctgac
      121 ctgcgcccac gtgacataca gatcgcgggc atcgcgaaac accgggtcgc gttggcaccg
      181 cgccagctct tgcctcatcg agccgcgtcc gacgtagaga acggccgaca ccctagcgat
      241 attaccgttg cgccagacga cgaatacgcc gtactggtcg atctgctcgg gatcgacggc
      301 atcgaaggcg ggccaggcgc cgcccggtgc cttgaaccaa tgtagctgca cttcgggccc
      361 cgttttcctt tgtagtaatt ctcgtaacgc cgctgcctgc ggtgcggcgc cccgggattc
      421 aacgcggtcg acctcagccg acgtccccat accagccgcg gtgcaagttc cgtgcgcggc
      481 cgggccgatg acggccaggg cgccgcgacc ggacgcggtt cttgcacgga atttgcagtt
      541 ccagggcttg cgcggcccga aagcccgcgg aaacacgacg gtcgtgcctt ggcactgacc
      601 gtcaagccgc gtgcggattg caaaaattgg aatcaacatt cggaaaagtt gccgagaacc
      661 catgaaacca gacgccgtct tcaggctcgt gaccgaaatg tcggacctgc agaagctcag
      721 cctgctgcaa ctgctcaaga tcggcgcgac gtcgccggag gtcaggtcgg tcgtgctcga
      781 atgccgcgac cgcggcctcg acgtcgacgg cacactggcc gaggtgttgc gccggtttgc
      841 ggacaagcgt atgcccgccg agccgaccgg ccgcgtgacg caccactagg acactggacc
      901 taggtccgat tccgcgttcc gccgcgcctc tgaaatagtt ctccgtgagg atggccctgc
      961 ggccatcgtg cgctcctgcg taccgccacc aaggagaacg tagcgtgaac aggccgatgt
     1021 cggtgcttct cgtgctagcc gccctgctgt ccagtgcttc cgttacggcg caggggacct
     1081 tggaccgcat ccgggagcgc ggcgagatca ggatcggcta tcgcggcgac gcgcggccgt
     1141 tgtcgttcga ggagaacggg gcgcccgcgg gctattccgt cgatctttgc aagcgcgtcg
     1201 cggccggcat cggcgagcaa ctgaagatgc ccgccatgaa ggcgacgtac gtcaaagtga
     1261 cgacggacaa ccgcttcgac gcgctcgtca agggcgacat cgacatcgag tgcggcgcaa
     1321 cgacgatcac gctcgggcgg ctcgagcgcg tcgacttcac gctgatgacg ttcgtcacgg
     1381 gcggcacgtt cttgtccaag gccgagaacc gcgtcggcgg cccggccgac ctcgccggca
     1441 agcgaattgc cgtgccgcgc ggcacgtcga ccgcgacggc gctgcaggct tacttgaagg
     1501 agaattccat cgacgcgcga gtcgtgacgg tcgacgaccg cggcgccggc atgtcgcgcc
     1561 ttacgcgcgg cgacgtcgat gcgctggccg gggatcaaat cgtgctgctc ggcgacgcgc
     1621 tcgacgtgct cgagacgaac aaggacgcga acttctcgtt cgccgacgcg ctgttctcgt
     1681 acgagccgta cggcttcatg gttcggcgca acgacgccga cttcaggctc gccgtcaacc
     1741 gcgagctcgc gaaggttttc cgcgacggcg accaagcgca gatctatcag aactgggtcg
     1801 gcagcaatgg cgtgaagcct tcgccgatgc tgatcgcgat gtatctcgtt cagggcttgt
     1861 cggagtagac ctcgcggccg cgcggcgtca ctgagcgctc gacagcctca tcagaatcaa
     1921 gccgcagacg atcagcgcgg ccgcggccac ccgcatcgcg tcggccggtt ctccgagcac
     1981 ggccacgccg accgcgaacg tcccgacggc gccgatgccg gtccagaccg tgtaggccgt
     2041 tccgagaggc agcgttcgca tcgcgagcga cagcagcacg aagctcacga tcatcgtcgc
     2101 gatcgtgatg accgagggca ggacgcgcgt gaagccgctc gactgcttca tgtagtacgc
     2161 ccagacgacc tcgaacacgc cggcgacgac taacaggatc caggccatga cggcacctcc
     2221 acttcgttac agtgccgggc cgtcccggac ttatgcccaa tcggttgggg gaggacgttg
     2281 cctcgcgtgc ccatggtagc gaacctaggt aagtctcaag gctgtttgag cccgtgcgtt
     2341 cgcgctggta cgctcgcgcg gatgggtcga tccttttcct tgcaattgca actggatcgc
     2401 atccgccgag gattcaactt ttgagggggc gacgacatgc ttaagcggac cttgggatct
     2461 cttctcgtgg gcggcgcgct gctcgcggcc ggctcgctgc aggctcacca ctcgctggcg
     2521 ggcgtctacg acatccgcgg gcagggcagt gtgtcgggag tgttgacgaa ggtcgcattc
     2581 acgaatccgc acggcgcgat gtacctcgac gtcacgaatg ccgacggcac ggtcacgaat
     2641 tgggtcatga cgacgggctc ggccaatacg ctcgcgaacc tcggcttcgg caccggcggt
     2701 ccgaacacgg tcaaagcggg cgacgcggtc acgatcacgt acttcccggc gcgtaacggc
     2761 aagccgctcg gcttcatccg ctcgatcgtt ctggccgaca agcgcgagat ccagatctcg
     2821 agcggcagca gctccgacta aagaacgcgt ctcgtaggcc cttctcggtc acggcggccc
     2881 cgcgttgctc gcagcgccgg ggccgttctt tttccgcaac cagttgttca cggttcgatg
     2941 agccggccga tcgcgcgtgc gcaacggccc cgtggatcgt gacgcagttc gtggcgccac
     3001 ggcggcccga gggtctttga tttagggcga gcatccgacc ggcctgaccg gcaccaactc
     3061 gcctgcgctg cggccagacg accgggcgtc cgaccgtcga gcgaggggta tgacgttgga
     3121 ggcactgaag ctccccggct atcagcacgg cgagcggatc cacgacagca gcgcggccgt
     3181 cgtctatcgc gcgaggcgcg tcagcgacgg tgcccgcgtc gtcatcaagc gctcgcaggg
     3241 acattccgtc tccgtccgtc agctcacgcg ctatcgcaac gagttcgagc tgctgcgctc
     3301 gctcgactgc cgcggcgtcg taaaggcgca cgatctgctg cgccacgacg ggcacgtcgc
     3361 gctcgtgctc gaggatttgc ccggcacgtc gttgcgcgcc tggatccagt cctcgcccga
     3421 ggcgccgatc ggcgagcggc tcgccatcgc gatcgagctc gccgagatcg tcgcgaacgt
     3481 gcacgccgcg aacgtgatcc acaaggacgt cagcagccac aacgtcgtct acgacgcgca
     3541 gaaggggcgc tgcacgttga tcgattttgg catcgcgacg cggttgcgca gcgaagagag
     3601 cgaattcaaa gcggcgggcg cgctcgaggg cacgctcgct tacatcgctc ccgagcagac
     3661 gggccgcatg aacaggagcc tcgaccatcg cgccgatctc tactcgctcg gcgtgacgct
     3721 ctacgagctc ttcacgggag agctgccgca cgagagcgcc gatcagctcg aaatggtgca
     3781 cttccacatt gccgtcaagc cggtgccgcc gcgcgagcgc cacgcgcgcg tgccagaggc
     3841 gctctcggag atcgtgctca agctgctgca aaaggagccg gagtatcgtt accagagcgc
     3901 ggccggtctc gtcgccgacc tgcgccgctg cctggccgcc gtcgacgcag gccggcccgc
     3961 ggagcgcttc gagctcggga gcgccgacgt cgtcgatcgc ttcgatccgc cgcagaagct
     4021 gtacgggcgt gccgcggaaa cgagcgcgtt gctcgcgagc ttcgagcgcg tcgcacgcgg
     4081 cggcgtcgag acggttacgg tcgcgggcca gcccggcatc ggcaagacgt cgctcgtcca
     4141 ggagatctac cagccgatca cgaagcggcg cggctacttc gtcgccggca agttcgacct
     4201 gctgcagcaa aacgtgccct tcagcgcgct cgtcgcggct cttcaagacc tcgtccagca
     4261 attgctgatg gaaggcgagg aggagatcgc ggcctggcgt gaggcgatcg acgccgccgt
     4321 tcacccgaac ggccagctca tcgtggacgt cgtccccgcg ctcgagctca tcatcggccc
     4381 gcagccgcgc gtcgccgagc tcgaggggct cgaagcgcag aaccgcttcc acttggcatt
     4441 ccagaatttc gtgcagctgt tctgcaagaa gtcgcacccg ctcgtgctgt tcctcgacga
     4501 catgcagtgg gccgactccg cgagcctgaa cctgataacg cggctcgtgt cggcacgcgc
     4561 gaccgaatcg ctgctgctcg tgctcacgta tcgcgacgcg gaggtcgagg ccaaccaccc
     4621 gttcctgctc gcggtcaagg agcagcgaaa gcaaggcgtt cccgtgctcg cgatcgagct
     4681 gaagccgctc ggcgttcccg aggtggccga gttcgtcgcc gacacgctgc atcaagatgc
     4741 gcggactgcg atgccgttgg ccgaggtcat ccagcagaag accggcggca acccgttctt
     4801 catgcgccag ttcttgcgcg cgttgtacgg cgcgcggctg atcacgttcg acggcgccgc
     4861 gaagcgcttc cgttacgacg tggcggccgt caagagcgcc gcgatcacgg aaaacgtggc
     4921 cgagctcttg gccacggagc tgcagcggct gcccgctagc actcgcgaaa cgctgcgtgt
     4981 cgcggccgtc atcggcggcc ggttcgagct ccgtctgctc gcgaaagtgc gtaaacaatc
     5041 gccggcggcg acgaacgaaa gcctgcggcc ggcggtcgaa gcgggcctcg tggtgccgct
     5101 gtcgggcctc gaaagcgtcg acgcgaacgc gctgcagtcg ccgctcgtct acgggcggtt
     5161 cggcttccgc cacgaccgcg tgcagcaggc cgcgtatgcg acgctgcccg agagcgcgcg
     5221 gccgcagctg cacctcgaga tcggccgagc gtggctcgcg atggcctctt cggcggagct
     5281 cgaggcacgt ctgttcgacg tcgtcgggca cttgaacgaa ggccgcggcg gcatcgtcaa
     5341 cgccgaagag cggcgccgtc tcgccgactt gaaccgccgc gccggcatca aggcgcgcga
     5401 cgctacggca tacgacctcg ccgtcagcag ctttcgcacc gcgatcgagc tcgacggcgc
     5461 gccggcgtgg cgcgaccgct actcggttca gttcgacacg catcggcggc tcgccgaggc
     5521 gctcggcctc acggcggact ccgcgggcgc gctcgcggtc atcgagcacg cgctcgagca
     5581 cgccgagccg ctgatcgacc ggacgcagct ctgcgcgatc aagacgaacg tgctgctgat
     5641 catggggcgc atccccgaag cgctggcctg cgggcgcgcg gccgcgcgcg agttcggcgt
     5701 cgacctgccc gagcagccgg aggaggtccg cgcgctgctg caacgcgaga tcggctcgat
     5761 ccggcagcgc gcggccgcgg tcggcatcga gaagtggctc gacctgcccg cgatggagga
     5821 cccgagccag atcgcgctga tgggcctgct catgcactgc ctgcccgcgg cgttccagac
     5881 cgaccaggaa agctacgcgc tgctctgctg cacgatggtc aggctgtcca tcgagcacgg
     5941 caactgcccg ctctcggcgc gcgcgtacgg gtcgttcgcg gcgctcctga gcagccagct
     6001 cagcgagtac gaagacgcgt atcgcttcgc gaagctcggc gtcgacctgg cgcacaagct
     6061 cggcgagacg tcggtgttgt cgggcgtcta cttcctgtgg gcgatgttcg catcgcactg
     6121 ggtcaagccc gtcgacgaaa gcatcgacct ctacgagcag gccgtccgct tcggcctcca
     6181 gagcggcgac cacgtgcatg ccggctacag cgtcgcccgg cgcttttcgc acttgcaggt
     6241 gcgcggcgcg ccgctcgcgg agctgcgcga ggagggacgt gccgcgctcg agacgctgca
     6301 tcggatcagc gacgccgcga atcgcgaatt tctccagccg cgggtcgagc tcatcgactg
     6361 gctgcgcggc gagcgcccgc acggcaacac gctgggcacc gccgagctcg acgagtctgc
     6421 gcgaacggcc gagattcaga agcgcggcaa tcggtcgttc gaggccgact ggttcatggt
     6481 gctcacgatc ctgcgctacc acgccgagga gttcgaagcg gcgcacgcgt acgccaagat
     6541 cgccgcgggc ctgcagccgt tttgcgccgg attcgtgacg cgcgcggagc acgcgctgta
     6601 ctacgcgctc gcgacggccg ccgtctatcc ggccgccgcc gccggagagc gcgcggcgca
     6661 cgatgcgacg ctggccgcga tacgcaagga gatggcgcgc tgggtccggc tgtgcgccgc
     6721 caacgtcgag cacatgcagc tgctcgtcga ggccgaatgc gctcggctgc gcgacgcgcg
     6781 catcgaggcg gcggattact acgaccgctc gatcgcggcc gcgcgcggca acggctacct
     6841 aggtatcgag gcgctcgccg cggagctcgc cggccgcttc tggctcgccg cgggcaagcc
     6901 cgatttcgcg cgcatttacc tcgagagggc gctcgacgcg tacgtcgcat ggggtgccgc
     6961 cggcaaggcc gccgatctgt cgtcgaagct cggcctcgtg ccggcgggga gcgcagcggc
     7021 ttccgcggct tcggcttcga cgacgctcgg ctcgcaaaaa cgctcggacg cgctcgatct
     7081 cgcgacgctg ctcaaggcga gccaggcgat tgccggcgag atcgtgctcg agcggctgct
     7141 cgtcgagctc atggacatca tgcgtgagaa cgccggcgcc gagtccgtcg tcatcgttct
     7201 cgaatcgaac ggcgagttcc tcgttcaagc tgtcaagacc gcgagcggcg tcgcgcgggt
     7261 cttggtcgcc gagccgctgc gctcctcggt tgcctgctcg cccgggatcg tgaattacgt
     7321 gctgcgcacg tcggagctcg tcgtgctcga cgacgcggcg ctgcacggca agtaccgcgg
     7381 cgacgcctac gtcgcgagcc gccggccgaa gtcgatcctg tgcgcgcccg tcgcgcacaa
     7441 gggcaagctc atcggcgcgc tatacctcga gaacaaccag gtcgccggcg cgttcacgcc
     7501 ggaccggctc gaggcgttga acatcttgat gtcgcaggtc gccgtgtcga tcgacaacgc
     7561 gacgctgtat agccggcaag agcagcagag ccgcacgatc
//
LOCUS       DASSNN010000677         9432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1128298, whole genome shotgun sequence.
ACCESSION   DASSNN010000677 DASSNN010000000
VERSION     DASSNN010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128298"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1565
                     /locus_tag="VFL84_12650"
     CDS             342..1565
                     /locus_tag="VFL84_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012484837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7609522.1"
                     /translation="MLARALRDFGDGFVAVLLPVYLVALGHTAFEVGVIATAALFGSA
                     VLTLLIGAIGARCDLRTLLLSASLLMAVSGIGYAVADEYWLLTLVALIGTINPSAGSA
                     NVFVPLEHTVLAEHVAPLERTRAFARYSLAGALAAACGSLAAAAPEWLATLGLEKLVA
                     FKLMFVAYAALGLANVVVYRGVPHRPRERIGSSRAPLGRSRPVVFKLAALFSLDAFAG
                     GFIVQSLLALWLLERFDLSLATASAVFFWMGVLAAFSFPVAAWLASRIGLVNTMVFTH
                     IPASVCLILAAFAPTLESTVALLLVRAVLGQMDVPARSSYVMAVVGEAERPAAASATA
                     VPRSLAAAASPAIAGMLFAAGLQLWPLLICGGLKIAYDVLLFAAFRRLEPPEERVTAT
                     STTTRSLLRGPERRR"
     gene            complement(1507..2241)
                     /locus_tag="VFL84_12655"
     CDS             complement(1507..2241)
                     /locus_tag="VFL84_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609523.1"
                     /translation="MFSKRSDVRTWHETSPRAFRAIALTAAACGGAPALAFPPYETTD
                     AETAGTSAIEFRLGLLDVEETGSDTERHTPLTNLNFGVGPHFEISSELERSADDHELD
                     GAALGFKWAAPRGTVSIGVETLALLPVQSAQSGAGIESQFLVTVEREQWTLHGNGGVF
                     YDPRNDDTERGWRASVLAEFPRDTLRPGVELFIRDSDALETEIQAGAGLIVSLERMEI
                     RTGLHVGLNDAAPDLGANFWLSWKWQ"
     gene            complement(2222..3199)
                     /locus_tag="VFL84_12660"
     CDS             complement(2222..3199)
                     /locus_tag="VFL84_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609524.1"
                     /translation="MRFLILLAALVCSVSRAQAPEPLKQIATVALPNVEGRIDHLAFD
                     AQRQRLFVAALGNDTVEVLDAATNMHLKSLPGFHEPQGIAIAADFGAVAVANGDTGTL
                     QLLDAETFATRWTVAIGGDADNVRYDAAAKELYVAAEGGLYRVDPPSGKAVRHIAIDG
                     HPESFQLETGGKRVFANLPGLVSSQVVVGDREAGSVVARWSSLGCRSNYPMALDETAM
                     RVFIGCRLPARLAVLDSRSGELTTSVNVVGDTDDLFFDAARQRVYVIGGEGFVDVLQR
                     RGDAIERTARVATRAGARTGLWVASNGRLYVAVPARDERGAEARVFEAQ"
     gene            complement(3285..4691)
                     /locus_tag="VFL84_12665"
     CDS             complement(3285..4691)
                     /locus_tag="VFL84_12665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET7609525.1"
                     /translation="MSFFALEHKPTRADFGALLRLAGPLVLIQVGTMLMGVVDTIMVG
                     HVSPAALASVALGHMYFFAISIFGMGVLFALDPIVAQGLGARDELAVRRGLQRGLVLA
                     LALSVPISLVLLTVRPVLELVGQPAEVIPAAAGFVHRNALSVLPFYVFVVLRQTLQAH
                     HRVLPIGITIVVANLVNVALNYLWIYGHFGFPAMGVLGSAWATTVSRWLMAALMLALG
                     WGTLKPYLRELAPNLLDLRPLERMLKLGFPIGAQMVLESTAFNVMALLMGWLGVAQVA
                     AHQIALNLASLAFMVPLGVSSAAAVIVGHAVGRGDAEGVRRSTVASLAVGAGFMLCTA
                     ALFILAPRPLAALYTRDAPVLALAALLLPLAGLFQVFDGLQVVAIGLLRGLGDTRMPM
                     IINVIGFWCIGMPVSLWLGFGVGYGAVGLWWGLVVGLVVVALFLIVRVRKREQHGLER
                     IIIDEHAKAAMRADPIAD"
     gene            complement(4763..5224)
                     /locus_tag="VFL84_12670"
     CDS             complement(4763..5224)
                     /locus_tag="VFL84_12670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET7609526.1"
                     /translation="MPQATWDDLAAGRGCAFDLPRADVTSHWDLVGRLTASSWYLPVN
                     QMYRGHGILVFDPRHATRLDELSTAEWHAYAADLHRVVTAIVAVCKPDHMNVESLGNV
                     MPHLHWHVVPRYKRDGRWGQPIWAPDVKAQPERKLADDDRARLLAALRAAL"
     gene            5297..5950
                     /locus_tag="VFL84_12675"
     CDS             5297..5950
                     /locus_tag="VFL84_12675"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7609527.1"
                     /translation="MRDRSVVLELGAIAAAALVFVATFQVRPPYLDFVLAAAAVALIV
                     LSAARSRRLWELQRSVDPTGARGAWKAALVFTGAALVVLAGAGVLAARAAGTPATPRF
                     GNWHMLVVAALYFPWALLQQYIFMFYWFGRWLRLVPVPVAVALTAVAFAAVHFPRWPV
                     MGVTLVAGSVWALIYYRWRSIVPLAVSHALLGTALHYWVFGNDLLERWIPLLDRWLP"
     gene            6021..6464
                     /locus_tag="VFL84_12680"
     CDS             6021..6464
                     /locus_tag="VFL84_12680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HET7609528.1"
                     /translation="MTEQNDNVDRRSVLLGAGTAAAAGVVAAFGAKPTEAQGRVDQEC
                     MTIVYQNGPDVRFDFDYYINTHMPLIMRLYGKSISRFELRRGQPGADGAAPPYVATIT
                     IWIADGAAFDAAQREHQAGLRADVPKFTNAVLIAQRDRIVGTATS"
     gene            complement(6454..7011)
                     /locus_tag="VFL84_12685"
     CDS             complement(6454..7011)
                     /locus_tag="VFL84_12685"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HET7609529.1"
                     /translation="MAQNTIARKIVVVRGQRVMLASDLAILYEVTTGNLNLAVRRNAL
                     RFPPDFMFQLTKAEHASLRLQLASLELGRGRHPKYLPYAFTEAEYSHLEASGLRVGPT
                     VRFADTRCKVCAAGAAGTALGEWRYGCRPGDSGCKEADPSRTIGSGGRSRPMRARAWP
                     TVTANDRRLGAARAANCGGVRSSTT"
     gene            complement(7110..7751)
                     /locus_tag="VFL84_12690"
     CDS             complement(7110..7751)
                     /locus_tag="VFL84_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011377433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET7609530.1"
                     /translation="MPRFDLVIFDCDGVVVDSERLVFEVFGSFLRSLGVHLSDAETRE
                     QFLGRSLPDCMAIVERLRGKPAPAGALERYMAERDRVLAARVQAVEGIREVLDALTIP
                     YCIASSGGYDKMRITLGATKLMPLFEGRLFSATEVPRAKPAPDIFLFAAERMGASPAR
                     TAVIEDSVNGVLAGSAAGMTVFGYVDLTPAAKLMEAGASRTFTRMRELPALLE"
     gene            complement(7751..8182)
                     /gene="arfB"
                     /locus_tag="VFL84_12695"
     CDS             complement(7751..8182)
                     /gene="arfB"
                     /locus_tag="VFL84_12695"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HET7609531.1"
                     /translation="MAGDEGLRVSGSVTIPAAELELTAIRSQGAGGQNVNKVASAVQL
                     RFDAARSSALPPAARERLLALRDHRISADGVVVIKAQQFRSQEQNKAAALERLADLVR
                     RALVVPKVRRATKPTRASKARRLEDKTRRSRLKRERRTLDD"
     gene            complement(8191..>9432)
                     /locus_tag="VFL84_12700"
     CDS             complement(8191..>9432)
                     /locus_tag="VFL84_12700"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7609532.1"
                     /translation="CAAAAAIGIAALAATAQQSQGTPAAAAPPQQQQRGAAGGGGGIP
                     RPPNGIVVPPLGAGPFTYKTAEQEIRVVVYTRGLTRPWSAVWLPSGEMLVTERTGALR
                     VVRNGALDPTPVAGVPEVRVQGLSGLFDVALHPQFATNKFVYLTYNKPIAERQNGLGV
                     ARGVWDGKALTNVRDIFVTTDASSVSRLVFGRDGKLYVSTFGNAGDGSGAQNPMSHAG
                     KVLRLNDDGTVPKDNPFVGRDGYKPEIYTLGHRSTLGLIVHPVTGELWESENGPNGGD
                     EVNILKAGANYGWPLVSTGRSYPGPWQSKGFSREGFEDPHVYWVPSIAASGFTIYTGS
                     KLAGWKNNLLIGGMRMGEIPGTGHLERVVFNANWDEIRREMLLVDLRQRIRDVRQGPD
                     ELLYVLTDEDAGAILRIEPAS"
BASE COUNT         1492 a         3292 c         3082 g         1566 t
ORIGIN      
        1 ggtgtagagc ggctctgcag ccgctccgct gccgatcgcc aggccggcgg cggcaacaac
       61 gcttgtgctc tttctcatgc cgattctctt taaacctaag gtcgtagttt acgcccctga
      121 cttgccggcc catgaccggc aatgggccag tcgacagatg ctctcgggct tcgctagcat
      181 ctgcgagccg tcggggcttt tcgcaggcgc cccgcaactc gaagacggtc ttccgtccaa
      241 gcgctgcgca acacgtcgtg tcgacaggtt gccccatgcg cgaagatcgt cgttccaccc
      301 gccattcgac ctcacggcca gcccctgtcg cgtatcgcgt catgctggct cgcgcgctgc
      361 gtgacttcgg cgacgggttc gtggccgtgc tgctccccgt ctatctcgtc gcgctcggcc
      421 acacggcctt cgaggtcggc gtcatcgcga cggcggcgtt attcggctcg gccgtcctga
      481 cgctgctgat tggggccatc ggcgcgcgct gcgacctccg cacgctgctg ctctcggcgt
      541 cgctgttgat ggccgtgagc ggcatcggtt acgcggtcgc ggacgaatat tggctgctga
      601 cgctcgtggc cctgatcggc acgatcaatc cgtcggccgg ctcggccaat gtcttcgtgc
      661 cgctcgagca cacggtgttg gcggagcacg tcgcgccgct cgagcggact cgcgcgttcg
      721 cgcgctacag cctcgccgga gcgctcgctg ccgcgtgcgg gtctctcgcg gccgccgcgc
      781 cggagtggct cgcaacgctc ggcctcgaga agctcgtcgc attcaagctg atgttcgtcg
      841 cctacgcggc gctcgggctt gcaaacgtcg tcgtttatcg cggcgtcccg catcgaccgc
      901 gcgaacgcat cggaagctcg cgtgcgccgc tcggccggtc gcgccccgtc gtcttcaagc
      961 tcgctgcact gttcagcctc gacgcattcg ccgggggctt catcgtccaa tcgctgctcg
     1021 cgctctggct gctcgaacgt ttcgatttgt ccctcgcaac cgcgagcgcg gtcttcttct
     1081 ggatgggcgt gctcgccgca ttctcgttcc cggtggcggc gtggctcgca tcgcgcatcg
     1141 ggctcgtcaa tacgatggtg ttcacgcaca ttccggcgag cgtctgcctg atcctcgccg
     1201 ctttcgcgcc gacgctcgag tcgaccgtcg cgcttctgct cgtccgcgcc gtactggggc
     1261 aaatggacgt gcccgcgcgc tcgtcgtacg tgatggccgt cgtcggcgag gcggagcgtc
     1321 ccgccgccgc aagcgctacg gcggtgccgc gaagcctcgc tgccgcggcg agccccgcga
     1381 tcgccggcat gctgttcgcg gcgggcctgc aactctggcc gctgctgatt tgcggcgggc
     1441 tcaagatcgc ctacgacgtg cttctattcg cggcgtttcg ccggctcgag cctccggaag
     1501 aaagggtcac tgccacttcc acgacaacca gaagtttgct ccgaggtccg gagcggcgtc
     1561 gttgagaccg acgtgcaagc ccgtgcgaat ctccatgcgc tcgagcgaga cgatgagccc
     1621 cgcgccggcc tgtatttctg tctcgagcgc gtcggaatcg cgaatgaaaa gctccacgcc
     1681 gggccgcagc gtgtcgcgcg gaaactcggc gaggacgctc gcgcgccagc cgcgctcggt
     1741 gtcgtcgttg cgcgggtcgt agaagacgcc tccgttgccg tgaagcgtcc actgctctcg
     1801 ctcgaccgtg accaggaatt gcgactcgat gccggcaccg ctctgcgcgg actgcaccgg
     1861 caacagcgcc agcgtctcga cgccgatgct gaccgttccg cgcggagccg cccacttgaa
     1921 tccgagggca gcgccgtcca attcgtggtc gtccgccgag cgctcgagct ccgagctgat
     1981 ctcgaagtgc gggccgaccc cgaagttcaa attcgtcagc ggcgtgtgcc gctcggtgtc
     2041 cgagccggtc tcctcgacgt ccaacaggcc cagccgaaac tcgatcgcgc tggtacccgc
     2101 cgtctcggcg tcggtcgtct cgtacggagg gaacgcaagc gccggcgcgc cgccacaggc
     2161 cgctgctgtc agcgcgatgg ctcgaaaagc ccgcggcgac gtctcgtgcc aagtgcgaac
     2221 gtcactgcgc ttcgaaaaca cgtgcttcgg cgccccgctc gtctcgtgcc ggcactgcaa
     2281 cgtagagcct gccgttcgac gcaacccaca agcccgtgcg cgcgcccgcg cgggtggcga
     2341 ctctcgcggt cctctcgatg gcatcgccgc gccgctgcaa gacgtcgacg aagccttcgc
     2401 cgccgatcac gtagacgcgc tggcgcgccg cgtcgaaaaa caaatcgtcg gtatcgccga
     2461 cgacgttgac tgacgtcgtg agctcgcccg accgactgtc gagcacggcg aggcgcgcgg
     2521 gcagacggca gccgatgaac acgcgcatcg ccgtctcgtc gagcgccatc ggatagttgg
     2581 agcggcagcc gagcgacgac caacgtgcta cgacggaccc ggcttcccgg tcgccgacta
     2641 cgacctgcga gctgacgaga ccaggcagat tcgcgaagac acgcttgccg ccggtttcga
     2701 gctggaacga ctccggatgg ccgtcgatcg cgatgtgccg aacggccttg cccgacgggg
     2761 gatcgacgcg atagaggcct ccctcggccg cgacatagag ctccttcgcc gccgcgtcgt
     2821 aacgcacgtt gtcggcgtcg ccgccgatcg cgacggtcca gcgcgttgca aacgtctccg
     2881 cgtcgagcaa ctgcagcgtt cccgtgtctc cattggcgac cgcgaccgcg ccaaagtcgg
     2941 ccgcgatggc gatgccttgc ggctcgtgaa acccgggcaa cgacttcaga tgcatgttcg
     3001 ttgcagcgtc gagcacttcg accgtgtcgt tacccaacgc ggcgacgaac aaccgctgcc
     3061 gctgcgcatc gaacgcgaga tgatcgatgc gaccctcgac gttcggcagc gctaccgtcg
     3121 cgatctgctt caacggctcc ggcgcttgcg ctcgcgagac cgagcatacg agcgcggcca
     3181 gcagaatcag gaaacgcatg actcgacctc cgtatcggac tgcggagcgc atcgacgatt
     3241 atgcgctcgc gcggcccggt cggtagatcc tccgcgattg cgtttcagtc ggcgatggga
     3301 tctgcgcgca tggccgcctt cgcgtgctcg tcgatgatga tgcgctcgag cccatgttgc
     3361 tcgcgcttgc gcacgcggac gatcaggaac aaggcgacga cgacgagccc gacgacgagc
     3421 ccccaccaca ggccgacggc gccgtagccg accccgaaac cgagccacaa gctcacgggc
     3481 atgccgatgc accagaagcc gatcacgttg atgatcatcg gcatgcgcgt gtcgccgagg
     3541 ccgcgcagca agccgatcgc cacgacttgg aggccgtcga acacctggaa caagccggcg
     3601 agcggcaaca gcagcgccgc gagcgcgagc acgggcgcgt cgcgcgtata gagcgccgcc
     3661 aacggccgcg gcgcgaggat gaacagcgcg gccgtgcaga gcatgaagcc ggcgccgacc
     3721 gcaagcgacg cgacggtcga gcgccgcacg ccctcggcgt cgccgcgtcc gacggcatgg
     3781 ccgacgatga cggccgcggc cgacgatacg ccgagcggca ccatgaacgc gagcgacgcg
     3841 agattcagcg cgatctgatg cgccgcgacc tgcgcgacgc cgagccagcc catcagaagc
     3901 gccatgacgt tgaacgccgt gctctcgagc accatctgcg cgccgatcgg gaagcccagc
     3961 ttcagcatgc gctcgagcgg gcggagatcg agcaggttcg gcgcgagctc gcgcaagtag
     4021 ggcttcagcg tcccccagcc gagcgcgagc atcagcgcgg ccatgagcca gcggctcacg
     4081 gtcgtcgccc atgccgagcc gagcacgccc atcgccggaa agccgaagtg gccgtagatc
     4141 cacagatagt tcagcgcgac gttgacgaga ttagctacga cgatcgtgat gccgatcggc
     4201 agcacgcggt ggtgcgcttg cagcgtctgc cgcagcacga cgaagacgta gaacggcaac
     4261 accgacaacg cgttgcgatg cacgaagccg gccgccgcag gaatgacctc ggccggctgg
     4321 cccacgagct cgagcacggg ccgcaccgtc aacaggacga gcgagatcgg gacactgagc
     4381 gcgagcgcca gcacgaggcc gcgctgcaaa ccgcggcgca ccgcgagctc gtcgcgcgca
     4441 ccgagcccct gcgcgacgat cgggtcgagc gcgaacagca cgcccatgcc gaagatcgag
     4501 atcgcgaaga agtacatgtg cccgagcgcg accgacgcga gggccgcggg cgagacgtgg
     4561 cccaccatga tggtgtcgac gacgcccatc agcatcgtgc cgacctgaat cagcacgagc
     4621 ggcccggcga ggcgaagcag agcgccgaag tcggcgcgag tcggcttgtg ctcgagtgcg
     4681 aagaaagaca tcacggcaag aaacgcaaaa ggtgcgcagc ttagctcatc ggcgccggtg
     4741 ccgcctcacg agaacagtgc gctcagaggg cagcgcgcag cgccgcgagc agccgcgcac
     4801 ggtcgtcgtc cgcgagcttg cgctcgggct gcgctttgac gtcgggcgcc cagatcggct
     4861 gaccccagcg gccgtcacgc ttgtaacgcg ggactacgtg ccagtgcagg tgcggcatga
     4921 cgttgccgag cgattcgacg ttcatgtgat ccggcttgca gacggcgacg atggccgtca
     4981 cgacgcgatg cagatcggcc gcgtacgcat gccactcggc cgtcgagagc tcgtcgaggc
     5041 gcgtcgcgtg ccgcgggtcg aacacgagta tcccgtgacc gcggtacatc tgattgacgg
     5101 gcagatacca cgacgacgcc gtcagccggc cgacgagatc ccaatgcgag gtgacatcgg
     5161 cgcgcggcaa atcgaatgcg caccctcgcc cggcagccaa gtcgtcccaa gtcgcctgcg
     5221 gcatcgacat tgctccagtc gaatcgatcg ttcgaactgt agcaccatgg cgcgcgccgc
     5281 cagtatgctc aggcagatgc gcgatcgctc cgtagtgctc gagctcggcg cgatagccgc
     5341 cgcggcgctc gtcttcgtcg cgacgttcca ggttcggccg ccgtatctcg atttcgtgct
     5401 tgccgcggcg gccgtagctt tgatcgtgct cagcgccgcg cgtagccggc ggctctggga
     5461 gctgcagcgc agcgtcgacc cgaccggcgc gcgcggtgcc tggaaggcag cgctcgtctt
     5521 cacgggcgcc gcgctcgtcg tgctcgccgg cgcgggcgtg ctcgcggcgc gcgcggccgg
     5581 cacgcccgct actccgcgct tcggcaactg gcacatgctc gtcgtcgccg cgctctactt
     5641 cccgtgggcg ctgctacagc aatacatctt catgttctac tggttcggcc gctggctgcg
     5701 cctcgtgccc gttccggtcg ccgtcgcgct gacggccgtc gcgttcgcgg ccgtgcactt
     5761 cccgcgctgg cccgtcatgg gcgtgacgct cgtcgcgggc agcgtgtggg cactcatcta
     5821 ttaccgctgg cgaagcatcg tgccgctcgc cgtgtcgcac gcgctgctcg gcacggcgct
     5881 gcactattgg gtgttcggca acgatctgct cgagcgctgg attccgttgc tcgatcggtg
     5941 gctaccctag agcgcttgag cattgtgcgc gcgttcatgc cgtgctctag tagcggcaac
     6001 tcttttgggg agttccgaac atgacagagc aaaacgacaa cgtcgaccgc cgctccgtgc
     6061 tgctcggcgc cggtaccgcc gccgcagccg gcgtcgtcgc cgcgttcggc gcaaagccca
     6121 ccgaggcgca agggcgcgtc gatcaggagt gcatgacgat cgtctatcag aacggccccg
     6181 acgtgcggtt cgacttcgat tactacatca acacgcacat gccgctgatc atgcggctct
     6241 acggcaagtc gatcagccgc ttcgagttgc ggcgcgggca gcccggcgcc gacggcgccg
     6301 cgccgccgta cgtcgcgacg atcacgatct ggatcgccga cggcgctgcg ttcgacgccg
     6361 cgcagcgtga gcatcaagcg ggactccgcg ccgatgtgcc gaagttcacg aacgcggtct
     6421 tgattgcgca gcgcgaccgc atcgtcggca ccgctacgtc gtagaactgc gcactccgcc
     6481 acagtttgcg gcgcgcgccg cgcctagcct gcggtcattc gcagtgaccg tgggccacgc
     6541 gcgtgcacgc atcggccgag atcgaccgcc gcttcctatc gttcgtgacg gatctgcttc
     6601 tttgcagccg ctgtctccag gtcggcatcc gtacctccat tcgccaagtg ctgtccccgc
     6661 ggcaccggcc gcgcacactt tgcaacgggt gtcggcaaat cgcacagttg ggccaacccg
     6721 cagaccgcta gcttcgaggt gcgaatattc ggcctccgtg aatgcgtatg gcaggtactt
     6781 cgggtgtcgc cctctgccga gctctaagct tgcaagctgc aatcttagag acgcgtgctc
     6841 ggctttcgtg agctgaaaca tgaagtctgg cgggaaccgc aacgcgttgc ggcggaccgc
     6901 cagattcaaa tttcctgtgg ttacttcgta gagaattgcg aggtcgctcg cgagcatgac
     6961 ccgttggccg cggacgacta cgatctttcg ggcaatcgta ttctgagcca tcggttgcgg
     7021 tatgcgtcta ctcgccacct cccgagtatt gagggacgct gcgttttcac gcaggtttat
     7081 tttccggcca aagtgcaaac aaatctggtt tactccagca acgctggcag ctcgcgcatt
     7141 cgcgtgaagg tgcggctcgc gcccgcttcc atgagcttcg cggccggcgt caggtcgacg
     7201 tagccgaata ccgtcatgcc ggccgcgctt cccgcgagca cgccgttcac ggagtcttcg
     7261 atgaccgcgg tgcgcgccgg gctcgcgccc atgcgctcgg cagcgaatag aaagatatcg
     7321 ggggcaggct tcgcgcgcgg cacctcggtc gcgctgaaca gccgcccttc gaagagcggc
     7381 atcagcttcg tcgcgccgag cgtgatgcgc atcttgtcgt agccgccgct cgacgcgatg
     7441 cagtacggga tcgtcagcgc gtcgagcacc tcgcgaatcc cttcgacggc ctgcacgcgc
     7501 gcggcgagca cgcgatcgcg ctcggccatg taccgctcga gcgcgcccgc gggagcgggc
     7561 ttgccgcgga gccgctcgac gatcgccatg cagtcgggga gcgagcggcc gaggaactgc
     7621 tcgcgcgtct cggcgtcgct caaatgcacg ccgagcgacc gcagaaacga gccgaacacc
     7681 tcgaagacga ggcgctcgct gtcgacgacg acgccgtcgc agtcgaagat gacaaggtcg
     7741 aaacgcggca tcagtcgtcg agcgtgcgcc gctcgcgctt cagccgcgac cgccgcgtct
     7801 tgtcctcgag gcggcgcgcc ttcgacgccc gcgtcggctt cgtcgcgcgt cgcaccttcg
     7861 gcacgacgag cgcgcggcgc acgagatccg ccaaacgctc gagcgcagcc gccttgttct
     7921 gctcttggct gcgaaactgc tgcgccttga tgacgacgac gccgtcggcg gagatgcgat
     7981 ggtcgcgcag cgcgagcaag cgctcgcgcg cggccggcgg cagcgcgctc gagcgcgcgg
     8041 cgtcgaagcg cagctgcacg gcgctcgcga ctttgttgac gttctgcccg cccgcgcctt
     8101 gcgagcggat cgccgtgagc tcgagctcgg cggccgggat cgtcacgctg ccgctcacgc
     8161 gcagcccttc gtcgccggcc acggcgcgct tcagctcgcg ggctcgatgc gcaggatcgc
     8221 gccggcgtct tcgtccgtca gcacgtacaa gagctcgtcc ggcccctggc gcacgtcgcg
     8281 gatgcgctgc cgcagatcga cgagcagcat ctcgcggcgg atctcgtccc aattcgcgtt
     8341 gaagacgacg cgctcgaggt gccccgtgcc cggaatctcg cccatgcgca tgccgccgat
     8401 cagcaggttg ttcttccagc ccgcgagctt cgagcccgta tagatcgtga agcccgaagc
     8461 cgcgatcgac ggcacccagt agacgtgcgg atcctcgaat ccttcgcgcg agaacccttt
     8521 cgattgccac ggccccggat acgagcggcc cgtgctcacg agcggccagc cgtagttcgc
     8581 gccggctttt agaatgttga cctcgtcgcc gccgttcgga ccgttctcgc tctcccagag
     8641 ctcgcccgtc acgggatgca cgatgagccc aagcgtgctg cggtgaccga gcgtgtagat
     8701 ctcgggcttg tacccgtcac gccctacgaa cgggttgtcc ttaggcacgg tgccgtcgtc
     8761 gttcaaacgc agcaccttgc cggcgtggct catcgggttc tgcgcgccgc tgccgtcgcc
     8821 ggcattgccg aacgtgctga cgtagagctt gccgtcgcgg ccgaacacga gacgcgagac
     8881 gctgctcgcg tcggtcgtga cgaagatgtc ccgcacgttc gtcagcgctt tgccgtccca
     8941 tacgccgcgc gcgacgccga gcccgttctg ccgctccgcg atcggcttgt tgtaagtcaa
     9001 ataaacgaat ttgttcgtcg cgaactgcgg atgcagcgcg acgtcgaaga gacccgacaa
     9061 gccctgcaca cgcacctcgg gcacgcccgc gacgggcgtc ggatcgagcg cgccgttgcg
     9121 cacgacgcgc agcgcgccgg tgcgctcggt cacgagcatc tctccgctcg gcaaccaaac
     9181 ggcgctccac ggacgcgtca agccgcgcgt gtagacgacg acgcggatct cttgctccgc
     9241 cgtcttgtaa gtgaacggcc ccgcgccgag cggcggtacc actatcccgt tcggcgggcg
     9301 cggaattccg ccgccacccc cggccgcccc gcgctgctgc tgttgcggcg gtgcggccgc
     9361 agccggcgtg ccctgggatt gctgcgcggt tgcggccagc gccgcgatcc caatcgccgc
     9421 tgcggcggcg ca
//
LOCUS       DASSNN010000678         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1863616, whole genome shotgun sequence.
ACCESSION   DASSNN010000678 DASSNN010000000
VERSION     DASSNN010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863616"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFL84_12705"
     CDS             complement(<1..571)
                     /locus_tag="VFL84_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609533.1"
                     /translation="MLRWLSVVVLFVAAGAQAQLDGALISLEHPAIGYRTEPLDNPVA
                     ALQERLTEGKTQLAFDARPGIGYLPAVLRALEVPLESQLLVYSKTSLQQRLITPERPR
                     ALYFNDELALGSVQDGMIEIAVQDAKQGAVFYTLQRVDEQPRLERQGSCLGCHYAYAT
                     LGVPGFFARSVPTAKDGQILPWLGNALVDH"
     gene            671..2365
                     /locus_tag="VFL84_12710"
     CDS             671..2365
                     /locus_tag="VFL84_12710"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7609534.1"
                     /translation="MSTKTLASIVPVALATTLSAFAQPAPQPPSQPCPAVRDVLLTNG
                     RIHTMDVSDNVVASVRIVGDRFAEVGTTRMPTSRCTRTIDLGGRTAVPGLIDNHNHIV
                     LLGLRPGHDTRLESATSIGDVLAMLAARAAALPSGEWITSIGGFDINQFVRPPGMPRF
                     PTLAELDSVTPNHPVYIQMSFSGPSVTNSLGKRFFEQAGIEVGADGAIAGGFQTPNPT
                     TRALFELRKLQTLADQKRGTQDAMRYAASLGLTTHLDEGGFPATGTAADAAAHFDRYR
                     AYDALLALYAEGALTQRIRLNFLHMETDPATPELVARLKNVFPHYGDDLLRIVGIGEF
                     TAGASPILTEASEPWRNGTRRVAEAGWRNENHSLTPGDFKVIIDEWTQLNASLPAPGI
                     RNQRWVVAHAPFITQEYIDKLKALGGGVSVLGGWRYISGTAQQNGPPFRMLQQSGIPI
                     GMSSDGMQISPMNPWLGLYYVVTGKNARGEVINAGQTLGRAAGLRLYTAANGWFLSEE
                     GQLGSIEPGKYADVAVLSDDYFDSRAVSDEEIKRITSVLTLVGGQVVFGEPDRLGR"
     gene            complement(2438..>2784)
                     /locus_tag="VFL84_12715"
     CDS             complement(2438..>2784)
                     /locus_tag="VFL84_12715"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7609535.1"
                     /translation="RARTEEKRAQLQQLTLEREKLRDAIAAEVRESYARVVSLKSQVG
                     LADVAVAHAQRAYELNRTRIFDQQGLPLEALAAMQTLASAELGALEARAGLAVAQLRL
                     HTVLGNPIAGAL"
BASE COUNT          476 a          954 c          906 g          448 t
ORIGIN      
        1 cgtgatcgac gagcgcattg ccgagccaag gcaggatctg cccgtccttg gcggtcggca
       61 cgctgcgcgc gaaaaacccg ggcacgccga gcgtcgcata cgcgtagtgg cagccgagac
      121 agctgccctg ccgctccaac cgaggctgct cgtccacgcg ctgcaacgta tagaacacgg
      181 cgccctgctt cgcgtcttgc acggcgatct cgatcatccc gtcttgcacg gacccgaggg
      241 cgagctcgtc gttgaagtag agcgcgcgcg ggcgttccgg cgtgatcaag cgctgctgca
      301 acgacgtctt cgagtagacg agcagctgcg actcgagcgg cacctcgagc gcgcgcagca
      361 cggcaggcag atagccgatg ccggggcgag cgtcgaatgc gagctgcgtc ttgccttccg
      421 tcagacgttc ttgcagagcg gctacgggat tgtcgagcgg ctccgtccgg tagccaatcg
      481 caggatgctc gagcgagatc agagcgccgt cgagctgcgc ctgcgcgccc gcggctacga
      541 agagaacgac gacggaaagc caacgcaaca tcgaggacgt atcggctaca cgacgcgact
      601 cagcatacac ttcgagcctc ggattatcgc gcgttggggt aaagtaccac caacaatcag
      661 ggggcttcgc gtgtcgacca aaactttggc ttcgatcgtt cccgtcgcgc tcgcgacgac
      721 gttgtcggca ttcgcgcaac cggcgccgca accgccgtcg cagccttgtc ccgccgttcg
      781 cgacgtgctg ctgacgaacg gccgcattca cacgatggat gtgtccgaca acgtcgtcgc
      841 gtcggtccgc atcgtaggcg accgcttcgc cgaagtcggc acgacgcgca tgccgacgtc
      901 gcgctgcacg cgcacgatcg acctcggagg gcggactgcc gtgcccggcc tcatcgacaa
      961 tcacaatcac atcgtgctgc tcgggcttcg gcccggccac gacacgcggc tcgagtcggc
     1021 cacgtcgatc ggcgacgtgc tcgcgatgct cgcggcgcgc gcggctgcgc tgccgagcgg
     1081 cgagtggatc acctcgatcg gcggcttcga catcaatcag ttcgtgcggc cgcccggcat
     1141 gccgcgcttt ccgacgctcg ccgagctcga ctcggtgacg ccgaatcatc ccgtttacat
     1201 ccagatgtcg ttctcggggc cgtccgtcac gaactcgctc ggcaagcgct tcttcgagca
     1261 ggccggcatc gaggtcggcg ccgacggcgc gatcgcgggc gggttccaaa cgccgaaccc
     1321 cacgactcgc gcgctgttcg agctgcgcaa attgcagacg ctcgcggatc aaaaacgcgg
     1381 cacgcaagac gcgatgcgct acgcggcgag cctcgggctc accacgcatc tcgacgaagg
     1441 gggcttcccg gccacgggca ccgcggccga tgccgccgcg cacttcgatc gttatcgcgc
     1501 ctacgacgcg ctgcttgcgc tctatgccga gggcgcgctg acgcagcgca tccgcttgaa
     1561 cttcttgcac atggagaccg acccggcgac gcccgagctc gtcgcacgct tgaagaacgt
     1621 gtttccgcac tacggcgacg atcttctcag gatcgtcggc atcggcgagt tcactgccgg
     1681 cgcgtcgccg attctcacgg aggcgagtga gccttggcgc aacggcacgc ggcgcgtcgc
     1741 ggaagccggc tggcgcaacg agaaccactc gctgacgccg ggcgacttca aagtcatcat
     1801 cgacgagtgg acgcagctga acgcgtcgct gccggcaccc ggaatccgca atcaacgttg
     1861 ggtcgtcgca cacgcgccgt tcatcacgca ggagtacatc gacaagctga aagcgctcgg
     1921 cggcggcgtc agcgtgctcg gcgggtggcg ctacatcagc ggcaccgcgc agcagaacgg
     1981 cccgccgttc cgcatgctgc agcaaagcgg catcccgatc ggcatgagct ccgacggcat
     2041 gcagatctcg ccgatgaatc cgtggctcgg cctctactac gtcgtgacgg gcaagaacgc
     2101 gcgcggcgag gtcatcaacg cgggacaaac gctcggccgc gccgcgggtc tgcgcttgta
     2161 cacggcagcg aacggctggt tcttaagcga agaaggccag ctcggctcga tcgagcccgg
     2221 caagtacgcg gacgtcgccg tgctctcgga cgactacttc gattcgcgcg cggtcagcga
     2281 cgaggagatc aagcgcatca cttcggtgtt gacgctcgtc gggggacagg tcgtcttcgg
     2341 cgagcccgac cggctgggcc ggtaggtgct cagcggcacc gccgatttct gtcatcaagc
     2401 tcgcctcacg gctgcggctg gtgggtctcc gatgccgcta cagcgcgccg gcaatcggat
     2461 tgccgagcac cgtatgcagc ctgagctgtg cgacggccag gccggcgcgc gcctcgagcg
     2521 cgccgagctc ggcactcgcg agcgtctgca tcgcggccag cgcctcgagc ggcaggcctt
     2581 gttgatcgaa gatgcgcgtg cgattgagct cataagcacg ctgcgcgtgc gcgactgcga
     2641 cgtcggctaa cccgacctgg ctcttcaggc tgacgacccg cgcgtaagat tcgcgcacct
     2701 cggcggcgat cgcatcgcgc agcttctcgc gctccaacgt cagctgctga agctgcgcgc
     2761 gcttctcctc ggtccgcgca cgct
//
LOCUS       DASSNN010000679         3538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_620125, whole genome shotgun sequence.
ACCESSION   DASSNN010000679 DASSNN010000000
VERSION     DASSNN010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620125"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFL84_12720"
     CDS             complement(<1..623)
                     /locus_tag="VFL84_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET7609536.1"
                     /translation="MGRLFERASLSWPIVLAAVLAAGCDRASPPEDSVPQAARDVSQP
                     EGWADDLAMPTPKDLNPDPNVLEINLEARVADVELLPGTTTPAWTYNGTVPGPMIRGK
                     VGDRVIVHFKNSLPEETTIHWHGIRVPNEMDGAPGATQQPIATGDEFRYEFVLKDAGT
                     YWYHPHTDSSAQVGRGLYGAFVVDDPGDPKAFGDDLVMILSDIGLDE"
     gene            887..1264
                     /locus_tag="VFL84_12725"
     CDS             887..1264
                     /locus_tag="VFL84_12725"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET7609537.1"
                     /translation="MPSKDDKRERITAICRALPGAELEDKGDHAIYRVRGKVFAYFLD
                     DHHSDGIVSVCVKSELGENEDRARLEPQRFYLPAYIGSRGWFGMRLDRGRVSWREVEE
                     IVERSYRLTAPKSLLKRLDPAKP"
     gene            1345..1776
                     /locus_tag="VFL84_12730"
     CDS             1345..1776
                     /locus_tag="VFL84_12730"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET7609538.1"
                     /translation="MSWWGWCIVGALLLAAELFAVDAQFYLIFLGVAAFVVGLLGVAG
                     VDLPSWAQWLTFGALSIAAMLTVRKQLYERLRKRPLGNVATDVAQRVRIPEALPPGKT
                     LRVEYRGSGWTALNVGDRPIPAGEEVLIEGVDGLTLRVRLN"
     gene            1792..2739
                     /locus_tag="VFL84_12735"
     CDS             1792..2739
                     /locus_tag="VFL84_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HET7609539.1"
                     /translation="MDTVLVAVIAAVVVVVFVLAKTAIVVPQQSAYIVEYVGKYSRTL
                     QAGFHILFPFIERVAYKHTLKEMAIDIPEQICITKDNVQVGIDGVLYMQILDAARASY
                     GIANYEFAISQLAQTTLRSQIGKIELDRTFEERATINANVVSELDKASEPWGVKVLRY
                     EIKSINPPRDVIQAMEKQMRAEREKRAVILTSEGERDAKINQAEGEKQRVIKESEAQR
                     QQQINVAAGEAEAILAVATATAEGLRRVAEAVAAPGGREAMQLRVAEDYVTQLGNLAR
                     TANTLVVPANLSDIASMIALATKVFDQTHAQGGTAPAVR"
     gene            complement(2736..>3538)
                     /locus_tag="VFL84_12740"
     CDS             complement(2736..>3538)
                     /locus_tag="VFL84_12740"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7609540.1"
                     /translation="TPQWLPYIGTTDVDAATDAAVRLGGRVQTAPTALPNGGRYAVLV
                     DPQGAAFGVHASAAPPQPETAPKPGEFSWHELATTIAPNAAFGFYAALFDWDLVAEHD
                     MGAMGMYLVFGRSGRQLGGMFDKGAEGKPGAAYWLGYVSVTDLEGTVERAKAARGSVL
                     MGPMDVPGGDRIAQLMDPYGAFFALHKPAAQGAAAETGKAAARAPAKKAAAKKPAARK
                     AAKKAPAKRAKPAARKKAKKAAKKKAARKPAKAKPKKKAAKKAKKRRR"
BASE COUNT          557 a         1146 c         1239 g          596 t
ORIGIN      
        1 ttctcgtcga ggccgatgtc ggacaagatc atgacgaggt cgtcgccgaa tgctttcggg
       61 tcgccgggat cgtcgacgac gaacgcgccg tagaggccgc ggccgacctg ggccgacgag
      121 tccgtgtgcg ggtggtacca atacgtgccg gcgtctttga gcacgaactc gtaacggaac
      181 tcgtcgcccg tcgcgatcgg ctgctgcgtg gcgcccggcg cgccgtccat ctcgttcggc
      241 acgcggatgc cgtgccagtg gatcgtcgtc tcctcgggca acgagttctt gaagtgcacg
      301 atgacgcgat cgccgacttt cccacggatc atcggccccg gcacggtgcc gttgtacgtc
      361 cacgccggag tcgtcgtgcc ggggagcagc tcgacgtcgg cgacgcgcgc ttcgaggttg
      421 atctcgagca cgttcgggtc ggggttcagg tccttgggcg tcggcatcgc gaggtcgtcg
      481 gcccagccct cgggttggct cacgtcgcgc gccgcctgcg gcacggagtc ctcgggcggg
      541 ctcgcgcggt cgcagccggc cgcgaggacg gcggccagca cgatcggcca agagagagaa
      601 gctcgctcga acagacgtcc cattccgttt cccattgagt cagcgtgggg cttctcgccc
      661 cggttggcca ttggtagatc tacggacccc gaccggaccg cgcaagcgcg gcgtcgcggg
      721 ctcggtgtct aaggagacgg ccgttggaaa gccggcctcg gcggaaattc tatcgggaag
      781 cattgccccg ccttatcgca gaagcgaata tgcggcgcgt tgtctgcgca ttttcgcggc
      841 tcctgcagaa atcttgaact cgaggcggcc gattcggccg ttgcgcatgc caagcaaaga
      901 cgacaaacgc gagcgaatca cggccatttg ccgggcgctg cccggcgccg agctcgaaga
      961 caagggcgac cacgcgatct accgcgtgcg cggcaaggtg ttcgcgtact tcctcgacga
     1021 ccaccatagc gacggcatcg tctccgtctg cgtgaagtcg gagcttggcg agaacgagga
     1081 ccgagcgcgg ctcgagccgc agcgctttta cctgccggcc tacatcggct cgcgcggctg
     1141 gttcggcatg cggctcgatc gcggccgcgt gagctggcgc gaggtcgagg agatcgtcga
     1201 gcggagttac cggctgacgg ccccgaagtc gctgctgaag cggctcgacc cggcgaaacc
     1261 ttgatcgcac ggccgtgtac tgcataatcg cgcgcgggat gggcgcgctc gcggttaggt
     1321 tccggtcgcg ggcaggagaa agccatgagc tggtggggtt ggtgcatcgt aggcgcgctg
     1381 ctactagccg cggagctgtt cgcggtcgat gcgcagttct acttgatatt cctaggcgtg
     1441 gccgcgttcg tcgtgggttt gctcggcgtg gccggcgtcg atttgccgag ctgggcacag
     1501 tggctgacgt tcggcgcgct ctccatcgcg gcgatgttga cggtgcgcaa gcagctctac
     1561 gaacggctcc gcaagcgccc gctcggcaac gtcgccaccg acgttgcgca acgcgttcgg
     1621 attcccgagg cactgccgcc gggcaagacg ctgcgcgtcg aatatcgcgg cagcggctgg
     1681 acggcgctca acgtgggcga ccggccgatc cccgcgggtg aagaagtcct catcgagggc
     1741 gtcgacggtc tcactttgcg tgtgcggctg aactagggcg gcggagaatc catggacaca
     1801 gtgttggtgg cagtcattgc ggcggtggtt gtcgtggtct tcgttctggc gaagacggcg
     1861 atcgtcgtgc cacagcaaag cgcctacatc gtggagtacg tcggcaagta cagccgcacg
     1921 ttgcaagccg gattccacat cttgtttccg ttcatcgagc gcgtcgcgta caagcacacg
     1981 ctcaaggaaa tggcgatcga tatcccggag cagatctgca tcacgaagga caacgtgcag
     2041 gtcggcatcg acggcgtgct ctacatgcag atcctcgacg cggcgcgcgc gtcgtacggc
     2101 atcgcgaact acgagttcgc gatctcgcag ctcgcgcaga cgacgttgcg cagccagatc
     2161 ggcaagatcg agctcgaccg cactttcgag gaacgcgcca cgatcaacgc caacgtcgtc
     2221 agcgagctcg acaaggcgtc ggagccgtgg ggcgtcaagg tgctgcgcta cgagatcaag
     2281 agcatcaacc cgccgcgcga cgtgatccag gcgatggaga agcagatgcg cgcggagcgc
     2341 gagaagcgcg cagtgatcct gacgtccgag ggcgagcgcg acgcgaagat caaccaggcg
     2401 gaaggcgaga agcagcgcgt gatcaaggag tcggaggctc agcgccagca acagatcaac
     2461 gtggcggcgg gcgaggccga ggcgatcctc gcagtggcga ccgcgacggc cgagggcttg
     2521 cggcgcgtcg ccgaggcggt ggccgcgccg ggcggccgcg aggcgatgca gctgcgcgtc
     2581 gcggaggact acgtgacgca gctcggcaac ctcgcgcgca ccgcgaacac gctggtcgtg
     2641 ccggcgaact tgagcgacat cgcgagcatg atcgcgctcg cgacgaaggt cttcgaccag
     2701 actcacgcgc agggcgggac ggcgcccgcc gtgcgctagc ggcggcgctt cttcgccttc
     2761 ttcgcggcct tctttttcgg cttggccttg gccggcttgc gcgcggcctt cttcttggcc
     2821 gccttcttgg ccttctttct cgcggccggc ttcgcgcgtt tcgcgggcgc tttcttggcg
     2881 gccttgcggg ccgccggttt cttcgcggcc gcctttttcg cgggcgcgcg cgccgcggcc
     2941 ttgccggtct cggccgccgc gccctgtgcc gcgggcttgt gcaaagcgaa gaacgcgccg
     3001 tacgggtcca tgagctgcgc gatgcgatcg ccgcccggca cgtccatcgg gcccatcagc
     3061 accgagccgc gcgccgcctt cgcgcgctcg acggtgccct cgagatccgt gacgctgacg
     3121 tagccgagcc agtacgcggc gccgggcttg ccctccgccc ctttgtcgaa catgccgccg
     3181 agctgcctgc cgctgcggcc gaagacgagg tacatgccca tcgcgcccat gtcgtgctcg
     3241 gcgaccaagt cccaatcgaa cagcgccgca tagaagccga aagcggcgtt cggagcgatc
     3301 gtcgtcgcga gctcgtgcca agaaaactcg ccgggtttcg gcgcggtctc gggctgcggc
     3361 ggtgctgccg aagcgtggac gccgaatgcg gcgccctgcg ggtccacgag gacggcatag
     3421 cggccgccgt tcggcaacgc tgtgggagcc gtctgcacgc ggccgccgag acgcaccgcg
     3481 gcatccgtcg ccgcatcgac gtcggtcgtg ccgatgtacg gcaaccattg cggcgtgc
//
LOCUS       DASSNN010000680         6111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_978869, whole genome shotgun sequence.
ACCESSION   DASSNN010000680 DASSNN010000000
VERSION     DASSNN010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978869"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1190)
                     /locus_tag="VFL84_12745"
     CDS             complement(90..1190)
                     /locus_tag="VFL84_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET7609541.1"
                     /translation="MSVRSYVCGALAAWVMAGAPAAAEPGARQVPLPPGLASVWRVDG
                     RKVDAYSPAWSLAAFSTDGKLVGVSDDGGTRIYDASDGRLVRMLPAPYSTGQFAYSLA
                     ISSTGLVALGRVGGVDIHALEQRGEPAKFYCGGICGPISALAFSPNGAWLASQAARSM
                     RDTTPGIVDVLDLRAGIRGAELEASSTRAGVMFAGDGGTLIAANVTRVDGTGTFGMRR
                     FSSAADWRRTRDLVGAQVPRGSIGPYAFNERVAAYAFDGRVELREVATGALVWAQPFV
                     PPGLDSTADTPMKLDLVAFAPRGDLLLGYESPVSREGPGAIVLRRMTDGAVVAMYDVV
                     GVSALAVAPDSDRFMYTTGAGRTYTVVARVPR"
     gene            complement(1187..1981)
                     /gene="thyA"
                     /locus_tag="VFL84_12750"
     CDS             complement(1187..1981)
                     /gene="thyA"
                     /locus_tag="VFL84_12750"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET7609542.1"
                     /translation="MRQYLDLLRAIKERGVRKSDRTGTGTLSLFGYQMRFDLDAGFPV
                     LTTKKLHLRSIIHELLWFLKGDTNIQYLRDNEVTIWDEWADDNGDLGPVYGAQWRAWR
                     TADGRQIDQIQRALDLIRNEPSSRRIIVSAWNVGELEQMALAPCHALFQFHVAQGRLS
                     CQLYQRSADVFLGVPFNIASYALLIHMMAQQTNLKPGELVWTGGDCHLYTNHMVQTEL
                     QLTREPLPLPRLVIKRKPESLFDYRFEDFELLDYYAHGSIKAPIAV"
     gene            complement(1983..2777)
                     /gene="lgt"
                     /locus_tag="VFL84_12755"
     CDS             complement(1983..2777)
                     /gene="lgt"
                     /locus_tag="VFL84_12755"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET7609543.1"
                     /translation="MFTYPHFDPVAFSIGPLSVRWYGIMYLVGFVLGWLGARTHAKKP
                     WSRIKPENVDDIVFYVALGAIIGGRVGYMLIYGLSELIADPLSIFKVWQGGMSFHGGF
                     IGVLTAMWLYARHIKQPFLVVMDFVAPWTAFGLFAGRIGNFINGELWGKPTSPDAPWA
                     VIVDGQALHPTQLYEAFLEGIVLFVVLWLYSRKPRPPMATSALFLIVYGVSRFIVEFV
                     RVPDQQIGYLAFGWVTMGQVLSAPMIIAGVILLVLAYRRAGTTVEA"
     gene            2928..4955
                     /locus_tag="VFL84_12760"
     CDS             2928..4955
                     /locus_tag="VFL84_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET7609544.1"
                     /translation="MRNRLSEETSPYLKQHADNPVHWQAWNDEALEEARKSRKPILLS
                     VGYSACHWCHVMAHESFEDEATAKLMNDLFVNIKVDREERPDLDKIYQTSHQLMTRQA
                     GGWPLTVFLTPEQVPIFTGTYFPKERRFGLPAFREVLVAIDQYYREKADEIRERGASL
                     VEALRSLHVASADDFAQLSREPLMLALERLRANYDPEHGGFGGGPKFPHTTSLELLLA
                     HSRRAGGAGAQASAMVAHTLDRMAQRGLYDHLGGGFFRYSVDGAWAIPHFEKMLYDNA
                     QLLGLYADAYAATGTQRYASVARATADWVMRDMQSREGGYYSTLDADSEHEEGKFYGW
                     TPAQFDAALTREESALAKRVFGLDGPPNFEGKHWHLFLAEAPEAAAAALGLDVARAGE
                     LLETARAKLLAAREKRVWPGRDEKVLVSWNGLMIAGMARAARILADPRLADSATRAVD
                     FIRAELFSDGRLKATYKDGRARFAGYLDDYAFLAYGLLELLQTRWRDSDLAFARELAD
                     VLLTHFEDASGGFFFTADDHERLIMKPKPFVDEAAPAGNGIAALALIALGHLTGEQRY
                     LDAAERTVRAALHALKSYPDAHATLLRALEELLEPPKLVVLRGELDELEPWQRRLASA
                     YEPNRLAFAIPNGAKLSGLLAERAPRERPVAYVCEGMTCRAPVELREGAVL"
     gene            complement(5000..5905)
                     /locus_tag="VFL84_12765"
     CDS             complement(5000..5905)
                     /locus_tag="VFL84_12765"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7609545.1"
                     /translation="MAAASVAAQDAAAQTMYRYKDANGVWVYTDRKPGGEQDFAEQQI
                     ERRFEKPAVNLLQRSQENGVALIAQNTYFGPVQIAFRVRPIENVSPSTRRSGLTVLPP
                     RSETELVVVGKSAQDVDLRFDSDFQFIPGAPGVEHAPDQPYRLPYALSSSVRVSQAYP
                     DVRTHSDRSSQYAIDFVMPIGTDVFAARDGVVIEVASDFFEAGTDYQVDGPRANVVRV
                     LHDDGTMGLYVHLNWNTIRVVPGQRVRRGEYLADSGNTGFSTGPHLHFVVQRNEGGAL
                     VAVPLEFASAAGPITLTTGKTYTAY"
BASE COUNT         1058 a         2103 c         2040 g          910 t
ORIGIN      
        1 cccaccgtgc tcgccgcaag caacagaacc acgcaccacc gcgtcaccag cgttgtcacc
       61 gataccccgc ctcgtcggcg cgaccgcgtt cagcgcggca cgcgcgcgac tacagtgtac
      121 gtgcgcccgg cgcccgtggt gtacatgaac cgatcgctgt ccggcgcgac ggcgagcgcg
      181 ctgacgccga cgacgtcgta catcgcgacg acggcgccgt cggtcatgcg ccgcagcacg
      241 atcgcgcccg ggccttcgcg gctcacgggc gactcatagc cgagcagcag atcgccgcgc
      301 ggcgcgaagg cgacgagatc gagcttcatc ggcgtgtcgg ccgtcgagtc gaggcccggc
      361 ggcacgaacg gctgggccca gacgagcgcg cccgtcgcaa cctcgcgcag ctcgacgcgg
      421 ccgtcgaacg cgtacgcggc cacgcgctcg ttgaacgcgt aagggccgat cgagccgcgc
      481 ggcacttgcg cgccgacgag gtcgcgcgtg cggcgccagt ccgccgcgct gctgaagcgg
      541 cgcatgccga acgtgcccgt gccgtcgacg cgcgtgacgt tggccgcgat cagcgtgccg
      601 ccgtcgccgg cgaacatcac gccggcgcgg gtcgaggacg cttcgagctc ggcgccgcgg
      661 ataccggcgc gcaaatcgag gacgtcgacg atcccgggcg tcgtgtcgcg catgctgcgc
      721 gcggcctgcg atgcgagcca tgcgccgttc ggcgagaacg cgagcgccga tatggggccg
      781 cagattccgc cgcaatagaa cttggccggc tcgccgcgct gctcgagcgc atggatgtcg
      841 actccgccga cgcggccgag cgcgacgagg ccggtcgacg agatcgcgag cgagtatgcg
      901 aactgccccg tcgaatacgg cgcgggcagc atgcgcacga gacggccgtc gctcgcgtcg
      961 tagatgcgcg tgccgccgtc gtcgctgacg ccgacgagct taccgtcggt cgagaacgcg
     1021 gccagcgacc atgccggcga atacgcgtcg accttgcggc cgtcgacgcg ccagacgctc
     1081 gcgaggccgg gcggcagagg gacttggcgc gcgcccggct cggccgcggc tggcgcgccg
     1141 gccatgaccc acgcggccag cgcgccgcaa acatacgacc gcacgctcac accgcgatcg
     1201 gggctttgat cgagccgtgc gcgtagtagt cgagcagctc gaagtcttcg aaacggtagt
     1261 cgaacaggct ctcgggcttg cgcttgatga cgagccgcgg cagcggcagc ggctcgcgcg
     1321 tgagctgcag ctccgtttgc accatgtggt tcgtatagag atggcaatcg ccgcccgtcc
     1381 agacgagctc gccgggcttc aggttcgtct gctgcgccat catgtggatc aacagcgcgt
     1441 acgacgcaat gttgaacggc acgccgagga acacgtcggc gcttcgctgg tagagctgac
     1501 acgagagccg gccctgcgcg acgtggaact gaaacaacgc gtggcacggc gccaacgcca
     1561 tctgctcgag ctcgccgacg ttccaggcgc tgacgatgat gcggcgcgag ctcggttcgt
     1621 tgcggatcag gtcgagcgcg cgctggatct ggtcgatctg ccggccgtcc gccgtgcgcc
     1681 acgcgcgcca ttgcgccccg tagacgggac ccaagtcgcc gttgtcgtcg gcccactcgt
     1741 cccagatcgt gacctcgttg tcgcgcaagt actggatgtt cgtgtcgccc ttcaggaacc
     1801 agagcaactc gtggatgatc gagcgcagat gcagcttctt ggtcgtgagc acggggaagc
     1861 ccgcgtcgag atcgaagcgc atctggtagc cgaacagcga gagcgtgccc gtgcccgtgc
     1921 ggtcgctctt gcgcacgccg cgctctttga tcgctcgcag aaggtcgagg tactgtcgca
     1981 tgtcaggcct cgacagtagt gccggcacgc cggtacgcga gcacgagcaa aatgacgccg
     2041 gcgatgatca tcggcgccga caacacttgt cccatcgtga cccagccgaa cgcgagatag
     2101 ccgatctgct gatccggcac gcgcacgaac tcgacgatga accgcgacac gccgtagacg
     2161 atcaagaaca acgccgacgt cgccatcggc ggccgcggct tgcggctata gagccagagg
     2221 acgacgaaca agacgatgcc ttcgaggaac gcctcgtaga gctgagtcgg atgcagcgcc
     2281 tggccgtcga cgatgacggc ccatggcgcg tcggggctcg tcggcttgcc ccacagctcg
     2341 ccgttgatga agttgccgat gcggcccgcg aacaggccga acgccgtcca cggcgcgacg
     2401 aaatccatga cgaccaggaa cggctgcttg atgtggcgcg cgtagagcca catcgccgtc
     2461 aacacgccga tgaagccgcc gtggaacgac atgccgccct gccatacctt gaagatgctg
     2521 agcggatccg cgatcagctc gctcaacccg tagatcagca tgtagccgac gcgcccgccg
     2581 atgatcgcgc cgagcgcgac gtagaagacg atgtcgtcga cgttctcggg cttgatgcgg
     2641 ctccacggct tcttggcatg ggtgcgcgcg ccgagccagc cgagcacgaa cccgacgagg
     2701 tacatgatcc cgtaccagcg cacggacagc gggcctatcg agaacgcgac cggatcgaaa
     2761 tgcggatatg taaacagacg acaccaccga acgagtcaaa cgctcgctag tgtagccgcg
     2821 ccgccgcccg tgcgcagcgg cagcgcctgc ggcgacgccg gaagtcgtct tctcgttacc
     2881 gcaagctcgt cttctcggag ctggcgtgga aggtgcatag tcgacgcatg cgaaaccggc
     2941 tgagcgaaga gacgagcccg tacctgaagc agcacgcgga caaccccgtg cactggcaag
     3001 cctggaacga cgaagcgctc gaggaggctc gtaagtcccg caagccgatc ctgctgtcgg
     3061 tcggctattc cgcgtgccat tggtgccacg tcatggcgca cgaatcgttc gaggacgagg
     3121 ccacggcgaa gctcatgaac gacctcttcg tcaacatcaa ggtggaccgc gaggagcgcc
     3181 ccgacctcga caagatctac cagacgagcc atcagctcat gacgcgccag gccggcggct
     3241 ggccgctgac cgtgttcctg acgccggagc aagtcccgat cttcacgggc acgtacttcc
     3301 cgaaggagcg ccgctttggg ttgcccgcgt tccgcgaggt gctcgtcgcg atcgatcagt
     3361 attaccgcga gaaggccgac gagattcgcg agcgcggcgc gagcctcgtc gaggcgctcc
     3421 gcagcctgca tgtcgcgagc gccgacgatt tcgcgcagct gtcgcgcgaa ccgctcatgc
     3481 tggcgctgga gcgcctccgc gccaactacg accccgagca cggcgggttc ggcggcgggc
     3541 cgaagttccc gcatacgacg tcgctcgagc tcttgctcgc gcattcgcgg cgcgccggcg
     3601 gcgcgggcgc gcaagcgtcg gcgatggtcg cgcacacgct cgaccgcatg gcgcagcgcg
     3661 gcctctacga tcatctgggc ggcggcttct tccgctacag cgtcgacggc gcctgggcga
     3721 tcccgcattt cgagaagatg ctgtacgaca acgcgcagct gctcggcttg tacgccgacg
     3781 cgtacgcggc gacgggcacg cagcgctacg cgagcgtcgc gcgcgcgacg gcggactggg
     3841 tcatgcgcga catgcagtcg agagaaggcg gctactactc gacgctggac gcggattccg
     3901 agcacgagga aggcaagttc tacggctgga cgcccgcaca gttcgacgcg gcgctcacgc
     3961 gcgaagagtc ggcgctcgcg aaacgcgtct tcggcctgga cggtccgccg aacttcgagg
     4021 gcaagcattg gcacctgttc ctcgccgaag ctcccgaagc cgccgcggcg gcgctcgggc
     4081 tcgacgtcgc gcgagccggc gagctgctag agaccgcgcg cgcgaagctg ctcgcggcgc
     4141 gcgagaagcg cgtctggccg ggccgcgacg agaaggtgct cgtctcgtgg aacggcctca
     4201 tgattgccgg catggcgcgc gcggcgcgca tcctcgccga ccctaggctc gcggattccg
     4261 cgacgcgcgc cgtcgatttc attcgcgccg agctcttcag cgacggccgc ctcaaagcca
     4321 cgtacaagga cggccgcgcc cggttcgccg gctatctcga cgactacgcg tttctcgcgt
     4381 acggcttgct cgagcttctg cagacgcgtt ggcgcgacag cgaccttgcg ttcgcgcgcg
     4441 agctcgccga cgtgctgctc acgcacttcg aggacgcgag cggcggcttc ttcttcacgg
     4501 ccgacgacca cgagcgcttg atcatgaagc cgaagccgtt cgtcgacgag gccgcgccgg
     4561 ccgggaacgg aatcgccgcg ctcgcgttga tcgcgctcgg ccatctcacc ggcgagcagc
     4621 gctacctcga cgcggccgag cgcaccgtgc gcgcggcgct gcacgcgctc aagagctacc
     4681 ccgacgcgca cgcgacgctg ctgcgcgcgc tcgaggagct gctcgagccg ccgaagcttg
     4741 tcgtgctgcg cggcgagctg gacgagctcg agccttggca gcgcaggctc gcgtcggcct
     4801 acgagccgaa ccggttggcc ttcgcgatcc cgaacggcgc gaagctgtcg gggctgctcg
     4861 cggaacgcgc gccgcgcgag cggcccgtcg cctatgtctg cgagggaatg acctgccgcg
     4921 cgccggtcga gctccgcgag ggcgcagtcc tctaaaaggg gacagatcta ttttcgtccg
     4981 aaaatagatc tgtccccttt tagtaggccg tgtaggtttt gccggtcgtg agcgtgatgg
     5041 gaccggcggc gctcgcgaac tcgagcggca ccgcgacgag cgcgccgcct tcgttgcgct
     5101 gcacgacgaa gtgcaaatgc ggccccgtgc tgaagcccgt gttgccggaa tcggcgaggt
     5161 actcgccgcg gcgcacgcgc tgacccggaa cgacgcggat cgtgttccag ttcaagtgca
     5221 cgtacaggcc catcgtgccg tcgtcgtgca gaacgcgcac gacgttcgcg cgcgggccgt
     5281 cgacttggta atcggtgccg gcctcgaaga aatcgctcgc gacctcgatc acgacgccgt
     5341 cgcgcgccgc gaacacgtcg gtgccgatcg gcatcacgaa atcgatcgcg tactggctcg
     5401 atcgatccga gtgcgttcgc acgtccggat acgcctgcga gacgcgcacg gagctcgata
     5461 acgcatacgg caaacggtac ggctggtcgg gtgcgtgctc gacgccgggc gcgcccggga
     5521 tgaactgaaa gtccgagtcg aagcgcaggt cgacgtcctg ggcggacttg cccacgacga
     5581 cgagctccgt ctcgctgcgc ggcggcagca ccgtgagccc gctgcgccgc gtcgacggcg
     5641 acacgttctc gatcggccgc actcgaaacg cgatctgcac cggaccgaag tacgtgttct
     5701 gcgcgatcaa cgcgacgccg ttctcctgcg atcgctgcag caggttcacc gccggcttct
     5761 cgaagcgccg ctcgatctgc tgctccgcaa aatcctgctc gccccccggc ttgcggtccg
     5821 tataaaccca caccccattc gcatccttgt accgatacat cgtctgcgca gccgcatcct
     5881 gcgccgcgac ggaagccgca gccacagcga gccatagcac acgctttgag acgaagaacg
     5941 atgagctcat ggaaagatgc gtcaggatcg acgaagcact gtctcacgca cgaccgccga
     6001 cggcggcgac tctcctcaca ctaccccacc cccaccgcca ccgtcgacga agtcgacata
     6061 aaacaaacgt ccccgcggtg actgacgtga gggggtcgtc gccggggcct t
//
LOCUS       DASSNN010000681         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_978897, whole genome shotgun sequence.
ACCESSION   DASSNN010000681 DASSNN010000000
VERSION     DASSNN010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978897"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1529)
                     /locus_tag="VFL84_12770"
     CDS             complement(183..1529)
                     /locus_tag="VFL84_12770"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET7609546.1"
                     /translation="MFITKKHLSRRTLLRGGTAAIGLPLLSAMVPAATALAQTAAAAK
                     PRMGFFYLPHGAIMNNTRYGNEMNRWTPDALGSDFDLKPILAPFAPLKKYMTVVSGLG
                     NKPAESSAVHAIVPATWLSCEHPRQSHAPYGGVTVDQIAAKHIGQDTPLPSLEVATEE
                     EGGGAACDGTYGCSYGRTISFRTPTTPLPMEFDARKAFEKIFGRGKSDSERRAVSGDY
                     QSLLDMVLTEASKLKGTLGGEDRALVDDYLDSVREIERRLALLGQRDLTKIDLPDVPV
                     ARPSFDDLLRLQFDLIVAAFQANMTRIASFMMAAEVSNQSYAHVGVPDAFHPVSHHAD
                     NKASMEKLVLIQRYHTQVFADFMTKLTKIPDGDGGSILDNSIFLYGSNMSNSNQHNQF
                     PLPTLVFGGGAGKIKGHQHLVYPDRTPLANLLFTLLLRAGVPVEKVGDSTGELAEV"
     gene            complement(1531..>2079)
                     /locus_tag="VFL84_12775"
     CDS             complement(1531..>2079)
                     /locus_tag="VFL84_12775"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HET7609547.1"
                     /translation="DRTSPVLRGAWIMEQILAAPPNPPPPGVETDLAPAGEVPKSVRE
                     RLALHRTQTSCNQCHGVIDPLGQALESFDVIGEWRSRERDSGVAVDSTGTTSIGQPVN
                     SPEELRAALSSDPTLFVHTLTEKLLTFALGRGLEYYDMPVVRRIVADASAQGYSFEAI
                     VLGVARSVPFRMRSGDEEPAGG"
BASE COUNT          331 a          677 c          705 g          366 t
ORIGIN      
        1 gaccgtcgcg tcatccctct ctgccgccgc gatcaagtca tcggcggccg acgaagaaaa
       61 attgacggcg cacgccgcaa acgcgactac agcggcgagc tcggtgccgg tgttacggtt
      121 tcggttgaac cgaaaccgta acaccggcac ctcttcaggg gcgtctcggg cgagcgacaa
      181 ggctagacct ctgcgagctc accggtgctg tcgccgacct tctcgacggg cacgcccgcg
      241 cgcagcagca gcgtgaacag caggttcgcg agcggcgtgc gatcgggata gacgaggtgc
      301 tgatggccct tgatcttgcc ggcgccgccg ccgaacacga gcgtcggcaa cgggaactga
      361 ttgtgctgat tgctgttgct catgttgctg ccgtacagaa agatcgagtt gtcgaggatc
      421 gatccgccgt cgccgtccgg aatcttcgtg agcttcgtca tgaagtccgc gaacacctgc
      481 gtgtgatagc gctggatcag cacgagcttc tccatcgacg ccttgttgtc ggcgtgatgc
      541 gagacgggat ggaacgcgtc gggcacgccg acgtgcgcgt acgactgatt gctgacctcg
      601 gccgccatca tgaagctcgc gatgcgcgtc atgttcgcct ggaacgccgc gacgatcaga
      661 tcgaactgca gcctgagcaa gtcgtcgaag ctcggccgcg ccacgggcac gtcgggcaag
      721 tcgatcttcg tcagatcgcg ctggcccaag agcgcaaggc gccgctcgat ctcgcgcacg
      781 ctgtcgagat aatcgtcgac gagcgcgcgg tcttcgccgc cgagcgtgcc tttcagcttg
      841 ctcgcttccg taagcaccat gtcgagcaag ctttgatagt cgccggaaac cgcgcgccgc
      901 tcggagtcgc tcttgccgcg gccaaaaatt ttctcgaacg ctttgcgcgc gtcgaactcc
      961 atcggcagcg gcgtcgtcgg cgtgcggaac gaaatcgtgc ggccgtacga gcagccatag
     1021 gtgccgtcgc acgccgcgcc gccgccttct tcctcggtcg cgacctcgag cgagggcagg
     1081 ggcgtgtcct ggccgatgtg cttcgccgcg atctggtcga ccgtgacgcc gccgtacggc
     1141 gcgtggctct ggcgcgggtg ctcgcacgat agccacgtgg ccggcacgat cgcgtgcacg
     1201 gcggagcttt cggcgggctt gttgccgagg ccgctgacga cggtcatgta tttcttgagc
     1261 ggcgcgaacg gcgcgaggat cggcttcagg tcgaaatcgc ttcctaaggc gtcgggcgtc
     1321 cagcggttca tctcgttgcc gtagcgcgtg ttgttcatga tcgcgccgtg cggcagatag
     1381 aagaagccca tgcgcggctt cgcagcggcc gcggtttgcg cgagcgcggt cgcggcgggg
     1441 accatcgcgg acaggagcgg caagccgatc gcggccgtgc cgcctctgag cagcgtgcgg
     1501 cgcgacaagt gcttcttcgt gatgaacata tcagcctcct gccggctcct catctccgct
     1561 ccgcatgcgg aacggcacgc tgcgcgcgac gcccaagact atcgcctcaa aactatagcc
     1621 ttgggccgag gcgtccgcca cgatgcggcg cacgaccggc atgtcgtagt actcgaggcc
     1681 gcggccgagc gcgaacgtga gcagcttctc ggtcagcgtg tgcacgaaca gcgtcgggtc
     1741 gctcgacagc gccgcgcgca gctcctcggg gctgttcacg ggttggccga tcgaggtcgt
     1801 gcccgtcgag tcgacggcta cgccgctgtc gcgctccctc gagcgccatt cgccgatgac
     1861 gtcgaagctc tcgagcgctt ggccgagcgg atcgatgacg ccgtggcact gattgcacga
     1921 ggtttgcgtg cggtgcagcg cgagccgctc gcgcacggac ttcggcacct cgccggccgg
     1981 cgcgagatcg gtctcgacgc cgggcggcgg cggattgggc ggcgccgcga ggatctgctc
     2041 catgatccac gcgccgcgca gcacgggcga cgtgcgatc
//
LOCUS       DASSNN010000682         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_856395, whole genome shotgun sequence.
ACCESSION   DASSNN010000682 DASSNN010000000
VERSION     DASSNN010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856395"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFL84_12780"
     CDS             <1..221
                     /locus_tag="VFL84_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET7609548.1"
                     /translation="AFYHDQKIDLEELIREQFLLALPMKPLCDEACKGLCPICGANLN
                     TNPCDCKPSWVDPRLKGLSDLLKNAKDS"
     gene            238..417
                     /gene="rpmF"
                     /locus_tag="VFL84_12785"
     CDS             238..417
                     /gene="rpmF"
                     /locus_tag="VFL84_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET7609549.1"
                     /translation="MPNPKRRHSKTRTAKRRTHDALQPIGRGECPQCHEAKPPHQVCP
                     NCGYYRGRQVKPVEE"
     gene            590..1615
                     /gene="plsX"
                     /locus_tag="VFL84_12790"
     CDS             590..1615
                     /gene="plsX"
                     /locus_tag="VFL84_12790"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET7609550.1"
                     /translation="MRIAIDAMGGDVGPSVVVDGALVAARHLQVGLLLVGSAAVIEAE
                     LARHPAAGGLDVQIADAPEHVEMSESAAAALRRKPRASIRVAVDCVSRGRADAVVSAG
                     HTGASVMAAHAAFGPLRGVDRPALATIIPTRHDRAILLDSGATVGCRPQHLVQFAVLG
                     AGYAKVAIERSDPTVGLLSVGEEETKGNELTREAHQLLKHAPIRFIGNIEGRDVFAGN
                     ADVIVCDGFTGNITLKLSEGLVDTVEGLLHDELASTFGGRVGYALSRQAFRRFRRRVD
                     HSEYGGAPLIGLNGLCIVAHGQSSSKAIANAVTMAVRAVDADLVTRLRHDVAAFTGTR
                     EPESFRS"
     gene            1612..>2067
                     /locus_tag="VFL84_12795"
     CDS             1612..>2067
                     /locus_tag="VFL84_12795"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET7609551.1"
                     /translation="MIAFVFPGQGAQKVGMGKALADAFDICRETFAEADAALGEPLSA
                     LCYDGPDDRLMLTENTQPAILAMSTAVARLVKSRGIDAAFAAGHSLGEYSAHVAAGTL
                     SFADALRIVRQRGRYMQEAVPVGEGAMAAILGLDAAAVARACADAAEGQV"
BASE COUNT          359 a          669 c          700 g          339 t
ORIGIN      
        1 cggcgttcta ccacgatcag aagatcgatc tcgaggaact catccgcgag cagtttctgc
       61 ttgcgctccc gatgaagccg ctctgcgacg aggcgtgcaa gggcctctgt cccatctgcg
      121 gtgcgaacct gaacaccaat ccttgcgact gcaagccgtc ctgggtcgac ccgcggctga
      181 agggattgag cgacctgctc aagaacgcga aggacagtta acaacgagcc gactcacatg
      241 ccgaatccca aacgacgcca ttcgaagacc cgcaccgcca agcgccgcac gcacgacgcc
      301 ctgcagccga tcggccgcgg cgagtgtccg cagtgccacg aggcgaaacc gccacaccag
      361 gtctgcccga actgcggcta ctatcgcggc cgccaggtca agccggtaga agagtgaagt
      421 cgttggccgt ttgccgtaag ccgtttgccg taagccgttc gccgttctat ttgtggcgcc
      481 cttcgggcgc ggcatcagtt cagctagaac ggtagcggcg gcaaaccgtc aacggtaaac
      541 ggcaaacggc aaacggcaaa cggcaaacgg ccaacggcaa acgttcttca tgcgcatcgc
      601 tatcgacgcg atgggcggcg atgtcggacc gtcagtggtc gtggacggcg ccctcgtcgc
      661 ggcgcgccat ctccaggtgg gactgctgct cgtgggcagt gctgcggtga ttgaagccga
      721 actcgcgcgc catccggccg cgggtggcct cgacgtgcag attgccgatg cgcccgagca
      781 cgtggagatg tcggaatcgg cggcggcggc cttgcgacga aagccgcgcg cgtcgatccg
      841 tgtcgcggtg gactgcgtca gccgcgggcg cgccgatgcc gtcgtcagcg cgggacacac
      901 gggcgcgtcg gtcatggcgg cgcatgcggc gttcggcccc ttgcgcggcg tcgatcgccc
      961 ggcgctcgcg acgatcattc cgacgcgtca cgatcgagcg attctgctcg attcgggcgc
     1021 gacggtcggc tgccggccac agcacctcgt ccagttcgct gtgctgggcg ccggctatgc
     1081 gaaggtcgcc atcgaacgat cggatccgac cgtcggactt ttgtccgtcg gggaagaaga
     1141 aacgaagggc aacgaactca cgcgcgaggc gcatcaactc ttgaagcacg ctccaatccg
     1201 gttcatcgga aacatcgagg gacgcgacgt gttcgccggc aacgcggacg tcatcgtctg
     1261 cgacggattc acgggcaata tcacgttgaa gttgagcgaa ggcctcgtcg atacggtcga
     1321 aggcctcctg cacgacgagc tcgcgtccac gttcggcggc cgggtcgggt atgcgctgtc
     1381 gcggcaggcg ttccgccgtt tccggcgccg cgttgaccac tccgagtacg gcggcgcgcc
     1441 gctcatcgga ctcaacggac tctgcatcgt cgcccacggg cagtcgtcgt cgaaggcgat
     1501 cgcgaacgcc gtgacgatgg cggtgcgggc ggtggacgct gatctcgtga cgcgtcttcg
     1561 tcacgacgtc gccgccttca ccggaacccg ggaacccgag agcttccggt catgattgcg
     1621 ttcgtctttc caggccaggg cgcccaaaag gtcgggatgg gaaaggcgtt ggccgacgcg
     1681 ttcgacatct gccgcgagac gtttgcggaa gctgacgctg cgctcggcga gccgctgagc
     1741 gcgctgtgct acgacggtcc ggacgatcgc ctgatgctga ccgagaacac gcagccggcg
     1801 atcttggcga tgagcacggc ggtcgcccgc ctggtgaaat cccgcgggat cgacgcggcg
     1861 tttgccgccg gccacagcct tggtgagtac tccgcacacg ttgcggccgg tacgctatcc
     1921 ttcgcggatg ccttgcgcat cgtccggcag cgagggcgat acatgcagga agcggttccg
     1981 gtcggagaag gcgcgatggc cgcgatccta gggctcgacg ccgcggcggt cgcccgtgcg
     2041 tgcgcagacg ccgcggaggg ccaggtc
//
LOCUS       DASSNN010000683         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_856708, whole genome shotgun sequence.
ACCESSION   DASSNN010000683 DASSNN010000000
VERSION     DASSNN010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856708"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VFL84_12800"
     CDS             complement(<1..923)
                     /locus_tag="VFL84_12800"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7609552.1"
                     /translation="MAAFSFNEFYQRNRRVVIWVILFLLLWALRDFFAIVFLTFVLAI
                     IAAPLAEIGMRRLRLPHWAAVTLVYVFFLVVLVAFVRFVVPSVAIEVNRMIDNLPATE
                     ATLIETKNRLVRRYPALREPINGYMRSALSDQSLEEVERQLANDRQRLGLSAADVAAA
                     AESTDVPTGALAEYFNRQDQLLVNALIAVQRQRISQYAPTVTVMLYRATATTLLALLF
                     SYLILIDLNRLKAGIGRLRNSRVGDFYEEAAPPIGRFGILLGRAVEAQAAIAVINTLL
                     TLIGLLLLKIPLVAMLSVIVFVCSFVPVLGV"
     gene            complement(1022..2125)
                     /locus_tag="VFL84_12805"
     CDS             complement(1022..2125)
                     /locus_tag="VFL84_12805"
                     /inference="COORDINATES: protein motif:HMM:NF042665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6607 family protein"
                     /protein_id="HET7609553.1"
                     /translation="MRTLLAGVAVLVLLLSWPAHAEPRQYTFSWPFEEGTAMRPRGGT
                     TRGAPLTLATGPSAAWQAVQEPGLSKFERDRRAILAMAGGYRTTFDFLETVGFTPGFA
                     PSRPYQSWSTEYVYVVEDSGTFIRLQHLIVMFVADRDGKVQGPFVQKHWRQDWRYEDT
                     DLHVFVGRNRWVRQTRTPDEVLGLWSQAVFQVDDSPRYESLGEWEHYGSYSAWTSGRT
                     ARPLPRRESTVRSDYEILEGTNRHTLTPTGWVQEEDNLKLNLDEQGQPDADVPYLARE
                     VGVARYEHVVDFDFSAGDAYWQRTGAFWRDVRAAWDAVYAERDSFEYLEQADGEEMFV
                     PLFEHAERLAGGEPYDAAAAAEVIRRTFERHLR"
     gene            complement(2127..2864)
                     /locus_tag="VFL84_12810"
     CDS             complement(2127..2864)
                     /locus_tag="VFL84_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HET7609554.1"
                     /translation="MLFGLAYLDRVESGPVGDWRSASRAAVGLAPDPATTREPVVQVY
                     AARTVRWRGYFGVHTWVAIKPRDASAYTVYEVNGWRLRRSGSAVSVDHRSPDSRWFGE
                     RPDLLAELRGDGVDGVIARIEAAVESYPYADTYHVWPGPNSNTFVAHLLRAAPELRAD
                     LPAHAIGKDYLGPSFVARAPSGTGVQANVFGVLGVLGGVEEGVELNVLGLTFGVDPLD
                     LSLKLPMAGRVGWPREAPAPVALADEN"
     gene            complement(2955..>3178)
                     /locus_tag="VFL84_12815"
     CDS             complement(2955..>3178)
                     /locus_tag="VFL84_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609555.1"
                     /translation="YTSVSRIVYSLTNEAQPGGVLKLVPDSKDRRRKHIELDADALKR
                     MGSHVRALEKAMIDYYGSSVYKLKKDQPN"
BASE COUNT          558 a         1069 c         1050 g          501 t
ORIGIN      
        1 aatacgccga gcaccggcac gaagctgcag acgaagacga tgacggacag catcgcgacg
       61 agcggaatct tgagcagcag caagccgatc agcgtgagca gcgtgttgat cacggcgatc
      121 gcggcctgcg cctcgaccgc gcggccgagc aggatcccga agcgtccgat cggcggggcg
      181 gcctcctcgt agaaatcgcc gacgcgcgag ttcctcaagc ggccgatgcc ggccttgagc
      241 ctattcaggt cgatcagtat cagatagctg aagagcagcg cgagcaacgt cgtcgccgtc
      301 gcgcgataca acatcacggt gacggtcggc gcgtattggc tgatgcgctg gcgctgcacg
      361 gctatcagcg cattcacgag cagttgatcc tggcggttga aatactcggc cagcgctcct
      421 gtcggaacgt cggtgctttc ggccgcggcc gcgacgtcag ccgcgcttag gccgaggcgc
      481 tgcctgtcgt tggcgagctg ccgttcgacc tcctccaggc tctggtccga cagcgcgctg
      541 cgcatgtagc cgttgatcgg ctctcgcagg gcgggatacc gccgcacgag cctgttcttg
      601 gtttcgatca gcgtcgcttc ggtggccggc agattatcga tcatccgatt cacttcgatg
      661 gcgacgctcg gcacgacgaa ccgcacgaag gccacgagca cgacgaggaa gaacacgtag
      721 acgagcgtga cggcggccca gtgaggcagc cgcaaccggc gcatgccgat ctccgccagc
      781 ggcgccgcga tgatcgcgag cacgaacgtt aggaagacga tcgcgaagaa atcccgcagc
      841 gcccacagca gcaggaacaa aatgacccaa atgacgacgc gccgattgcg ctgatagaac
      901 tcgttgaagc tgaaggcggc cacggtttgc tgcgggaact ggcgttgttc tttcggcgac
      961 tggcgaaggc cggaacggct atggtagcgg aacgcccgtc ggcttgcggc aggacgcggc
     1021 ctcagcgcag atgccgctcg aacgtcctgc ggatgacttc ggcggcggcc gctgcgtcgt
     1081 agggctcgcc gccggcgagc cgctcggcgt gctcgaacaa cggaacgaac atctcctcgc
     1141 cgtctgcttg ttcgagatac tcgaacgagt cgcgctcggc gtagacggcg tcccatgccg
     1201 cgcgcacgtc gcgccagaac gcccccgtgc gctgccaata cgcgtcgccc gccgagaaat
     1261 cgaagtcgac gacgtgctcg tagcgggcga cgccgacctc gcgcgcgagg tacggcacgt
     1321 cggcatccgg ctgcccctgc tcgtcgaggt tgagcttcaa gttgtcttcc tcttgcaccc
     1381 agcccgtcgg cgtcagcgtg tgccggttcg tgccctcgag gatctcgtaa tcgctgcgca
     1441 cggtcgactc gcgccgcggc agcggccgag cagtgcgccc gctcgtccac gcggagtagc
     1501 tgccataatg ctcccactcg ccgagcgact cgtagcgcgg cgagtcgtcg acctggaaca
     1561 cggcttgcga ccagaggccg agcacttcgt cgggcgttcg tgtctgccgc acccagcgat
     1621 tgcggccgac gaagacgtgc agatcggtgt cttcgtaacg ccagtcctgc cgccaatgct
     1681 tctgcacgaa cggtccttgc acctttccgt cgcgatccgc gacgaacatg acgatcaggt
     1741 gctgcaagcg gatgaacgtg ccgctgtcct cgacgacata aacgtattcc gtgctccacg
     1801 attggtacgg acgcgacggc gcgaaccccg gagtgaagcc gacggtctcg agaaagtcga
     1861 acgtcgtgcg gtagccgccg gccatggcga ggatcgcgcg gcgatcgcgc tcgaatttcg
     1921 aaaggcccgg ctcttgcacc gcctgccatg cggcgctcgg ccccgtcgcg agcgtaagcg
     1981 gcgcaccgcg cgtcgtaccg ccgcgcggcc gcatcgcagt gccttcctcg aacggccagc
     2041 tgaacgtgta ctgccgcggc tcggcgtgcg cgggccatga gagcagtagg acgaggacgg
     2101 caactcccgc gagcagcgtg cgcatgttag ttctcgtcgg cgagcgcgac gggagccgga
     2161 gcctcgcgcg gccagcctac ccggccggcc atcggcaact tcagcgacag atcgagcggg
     2221 tcgacgccga acgtgagccc caagacgttg agctcgacgc cctcctcgac tccgccgagc
     2281 acgccgagca cgccgaatac attcgcctgc acgccggtgc cgctcggcgc gcgcgccacg
     2341 aacgaggggc cgaggtagtc cttgccgatc gcatgcgcgg gcaagtccgc gcgcagctcc
     2401 ggcgccgcgc gcagcaggtg tgcgacgaac gtgttcgagt tgggtccggg ccagacgtga
     2461 tacgtatccg cgtacggata cgactcgacg gccgcctcga tgcgcgcgat gaccccgtcg
     2521 acgccgtcgc cgcgaagctc cgcgagcagg tcgggtctct caccgaacca gcgagagtcg
     2581 ggcgaacgat ggtcgacgct gacggcgctg cccgaacggc gcagccgcca gccgttcacc
     2641 tcgtagaccg tgtaggcgga cgcgtcgcgc ggcttgatcg cgacccacgt atggacgccg
     2701 aagtacccgc gccagcgcac ggtgcgcgcg gcatagactt gaacgacggg ctcccgcgtc
     2761 gtggcgggat ccggcgcgag cccgaccgcg gcgcggctcg cgctacgcca atcgccgacg
     2821 ggccccgact cgacgcgatc gaggtaggcc aagccgaaca gcaacggcgg aattgaccat
     2881 aaccatttgg cgctcggacg cttcattccg agccaacata gacgcgccgg gtaaaccggg
     2941 caagcgtcct ccgctcagtt cggctggtct ttcttgagct tgtataccga gctgccgtag
     3001 taatcgatca tcgccttctc gagcgcgcga acgtgcgagc ccatgcgctt caacgcgtcc
     3061 gcgtcgagct cgatgtgctt gcggcggcga tctttgctgt ccggcacgag cttcagcacg
     3121 ccgcccggct gcgcctcgtt ggtcagcgag tacacgatgc gcgacaccga tgtgtacg
//
LOCUS       DASSNN010000684         6300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1247590, whole genome shotgun sequence.
ACCESSION   DASSNN010000684 DASSNN010000000
VERSION     DASSNN010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247590"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1528
                     /locus_tag="VFL84_12820"
     CDS             <1..1528
                     /locus_tag="VFL84_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7609556.1"
                     /translation="IRGQELAIRAALGAARGRIARTLLGESVLLGFMGGIVGLAVAAS
                     TLPLLLTLAGQSLPTALEVTIDATVLAFAPVISLAAGLLFGLIPVIKHAGARFATGLT
                     GMGRTHSAGRERHRARNALVIAQVGFALMLLVASGLMIRTFQSLRDVEPGFTEPESVL
                     MLRVLIPQSAVPDFPQSLRVQNDIVDRLRAIPGVSSVGFATRPPLGQFGPTGPFSLED
                     KPDAPPLGPVFRYASPDYFATLGTPLVAGREIEWPDLYGTAQLAVISQGFAEREWGSA
                     GAALGKRLRRSPTSPWIEVVGVVGDIRLHGVEQAAPDAIYLTSNEFVAQFAGRVAYFA
                     VRSERVATQGFLREIEQAVWSVNGSLPLGDVHTLGDAYDRSMARTSLTLVLLGITAAM
                     ALALGLVGIYGVLCYVVTQRTREIGIRLALGAQNAQLERLVLGHVLALVGAGVALGLG
                     GAALLTRLMESQLFGVTALDPLTYGAVAAILTATGALAGYLPARRATRVDPMTALRVE
                     "
     gene            1675..2151
                     /locus_tag="VFL84_12825"
     CDS             1675..2151
                     /locus_tag="VFL84_12825"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HET7609557.1"
                     /translation="MNRNWIIGIVVAVGAIFVRSQMQADRDATGAIVDAGSVDAFQVR
                     VGDCFDDGSAFEGDEVNSVAGVPCSKPHDNEVYAVFDVAEQSFPGEALGGMAHEGCLE
                     RFEAFVGKDYDSSSLDIATMYPSRESWQQQHDREVICAVYDVEAKKLTGSMKGTRL"
     gene            2259..3758
                     /locus_tag="VFL84_12830"
     CDS             2259..3758
                     /locus_tag="VFL84_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609558.1"
                     /translation="MRPTNKTLRAALFGTGALLATASVYAQINDRIRGAPSEANHFVE
                     TPRGWVHPKTPWGEPDIQAKLNMMQAAGVPLERCANSYRAALPPGAGAPPGPAGGVGA
                     PGQPCDMNKKWLTEEEYAQRMEAFRTRVDASRQFAEQGNLGRAMMTGLTDPAIPQRQT
                     NLIVDPPNGLLPPLTAEGKRRAMRAKSDWALPGETIAFEGPESFDSWDRCITRGMPSS
                     MMPYRYNGGFSIRQAPGYVIFDLEMIHESRVIPTNGGQHLPPEVKQWMGDSRGHWEGN
                     TLVVETTNYQEGAPIINLAVVGSPPGNRFPYSDQMKTTERIVRLNDKTWLYEIKTEDP
                     VILTAPFTVRYPMNHDDSYWWSEYACHEDNTIVRNYTVTSRHERANPTPEPPQAPVQV
                     APGLADALAGRWVGRPRIVTIDVDIELEFTKNADGTVNGKLIGTNLGPIDRPLRGFTI
                     VDNTVYFTLPNVDPWRFSGELGDGTITGVVASAQGGVPVTFRKQPSRAR"
     gene            3764..4450
                     /locus_tag="VFL84_12835"
     CDS             3764..4450
                     /locus_tag="VFL84_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609559.1"
                     /translation="MLKMTGRIGGLLLGAIAMPAFACDLPKVPLIPEKDKVGDQAPTI
                     SAATGAYFDGMRAYTDCTEALLDAAGGEAASPSFKGVINGRLTVAVAEAQAVQKLFQE
                     RVGAGQTAQAGSEAALRKLIEGLASGKPDYDAMTPEFAKQTRQMIRFQQPMIAAAGAI
                     KSIEFAGIDTEGRNIFQVTQEKSMTNARIALDADGKIEFALLRPAPTPGEKRPTATIP
                     TRSSGLKRDR"
     gene            complement(4483..5340)
                     /locus_tag="VFL84_12840"
     CDS             complement(4483..5340)
                     /locus_tag="VFL84_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609560.1"
                     /translation="MKLATALLVALIAGTAVHAQNRGSTGPFTGYEAEALSDVWPEIR
                     VAARFEDINWPTHGLTRAPGSPEAQRLLSANWNEIRREERFEDIDWDEYANNRYSQSS
                     RSGDRYGRLEGPFTSDEADALARVWPQIRVAARFEDITWPRHGLERAPGSAEARRLLS
                     SNWDEIRREERFEDIDWDRYYDSRYSSRSDRAERYGRLERGFPGTERDDNDSPFTREE
                     AADLSRVWSEIRQAARFEDINWRTHGLSGPPGDSEARRLTSRYWSELRRAARFEDIDW
                     QTTTGYRAR"
     gene            complement(5416..5787)
                     /locus_tag="VFL84_12845"
     CDS             complement(5416..5787)
                     /locus_tag="VFL84_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609561.1"
                     /translation="MNTDELFRQLEPPPGGAEAFARRLDEIAAERPSRRARVLAAAAA
                     VAAVALVTAIVLTRKPSDAPPVLVADDAPAVDIYNAPELDRLLGRPSRPAELMVMVNM
                     ETANVTKIETSNEKVRIYQIN"
     gene            complement(5777..>6300)
                     /locus_tag="VFL84_12850"
     CDS             complement(5777..>6300)
                     /locus_tag="VFL84_12850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7609562.1"
                     /translation="NTRLDMPSPDKSQWVDVYRRLEKPLYNVVYRVIWDAAESQDVVQ
                     ETFLRCWRRKDDIRADGFKALLFRTALNLASNRRRRTRVWRVVGVEALDDVADESDST
                     DVFSKPVREAFDNLPTTLRDVLLLTELAGMTYPEIAAALGIAEGTVGSRRTRALALLR
                     ERLDVQGVVSHEY"
BASE COUNT         1025 a         2095 c         2134 g         1046 t
ORIGIN      
        1 gattcgcggg caggagctcg cgattcgcgc tgcactcggc gccgcgcgcg ggcgcattgc
       61 gcgcacgttg ctcggcgaga gcgtgttgct cggcttcatg ggcggcatcg tcggcttggc
      121 ggtcgcggcc agcacgttgc cgcttttgct caccctcgcc ggacagagct tacccacggc
      181 gctcgaagtc acgatcgacg cgaccgtgct cgcgttcgct ccggtcatct cactggccgc
      241 cggtctgttg ttcggcttga ttcccgtcat caagcacgcg ggcgccaggt tcgcgacggg
      301 actgaccggc atgggcagga cgcatagcgc gggccgcgaa cgtcatcgcg cccggaacgc
      361 gttggtgatc gcgcaggtcg ggttcgccct catgctgttg gtcgcgtcgg gcttgatgat
      421 ccgcacgttt cagtcgttgc gcgacgtcga accgggcttc acggagcccg agagcgtgct
      481 gatgctgcgc gtgctcattc cgcagtccgc cgtgcccgac ttcccgcagt cccttcgagt
      541 ccagaacgac atcgtcgacc gcctccgtgc gattcccggc gtgtcatccg tcggtttcgc
      601 gacccggccg ccgctcggcc aattcgggcc gacgggcccg ttctccctcg aggacaagcc
      661 ggatgcgccg cccttgggtc ccgtgttccg ctacgcgtcg cccgattact tcgcgaccct
      721 gggcacgccg ctcgtggcgg gtcgtgagat cgagtggccc gatctctacg gcaccgcgca
      781 attggccgtg atctcgcagg gcttcgcgga gcgggagtgg ggctcggccg gggccgcgct
      841 cggcaaacgc ttgcgccggt cgccaacctc accgtggatc gaagtcgtcg gcgtggtcgg
      901 cgacatcagg ctccacggtg tcgagcaagc ggcgccggac gcgatatacc tgacctccaa
      961 cgagttcgtc gcgcagttcg ccggccgtgt cgcctatttc gccgtccgca gcgagcgtgt
     1021 cgccacgcag ggttttttgc gggagatcga gcaagcggtc tggtccgtga acggcagcct
     1081 gccgctcggc gacgtgcaca cgctcggcga cgcttacgac cgctcgatgg cgcgaacgtc
     1141 gttgacgctc gtcctgctcg gcatcaccgc ggcgatggcg ctcgcgctcg gcctcgtcgg
     1201 catctacggc gttctctgct acgtcgtcac gcaacgcacg cgtgagatcg gcattcgcct
     1261 ggcgctcggc gcgcaaaacg cgcagctcga gcgcttggtg ctaggccacg tgctcgcgct
     1321 cgtcggcgcc ggcgtggcgc tcggcttggg cggcgcggcg ttgctgacgc gcctcatgga
     1381 gtcgcagctg ttcggcgtga cggcgctcga cccgctgacg tacggcgccg tcgcggcgat
     1441 tctgacggcg acgggcgcgc tcgccggcta tttgcccgcg cggcgcgcga cgcgcgtcga
     1501 tcccatgacg gcgcttcgcg tcgaataaga aaagcaccgc catcgtccgc gtctcattat
     1561 ggaagatgaa cgcgcgcgcc tacgcttggg cgcgcagaga gcgagacctg cgtccttccg
     1621 cggccgaaag tggcgggcaa gaattgccgc cgattcatcc cttggggggc cgtcatgaac
     1681 cggaactgga ttatcggcat cgtcgtcgcc gtcggcgcga tcttcgtcag gtctcaaatg
     1741 caggccgatc gtgacgcgac gggcgccatc gtcgacgcgg gaagcgtcga cgcgttccaa
     1801 gtgcgcgtag gcgactgctt cgacgacggc tccgcgttcg agggcgacga ggtcaacagc
     1861 gtggcgggag tgccgtgctc gaagccgcac gacaacgagg tctacgccgt gttcgacgtc
     1921 gccgagcaga gcttccccgg cgaagccctg ggcggcatgg cgcacgaggg ttgcctcgag
     1981 cgcttcgagg cgttcgtcgg caaggactac gattcgtcgt cgctcgacat cgcgacgatg
     2041 tatccgtcgc gcgaaagctg gcaacagcag cacgaccgcg aggtcatctg tgccgtctac
     2101 gacgtggaag cgaagaagct gacgggcagc atgaagggca cgcgcctata gcgtcgagct
     2161 gcgcgcccga gccggcccct cgtcgggtga tggacggccc cgcgccgtaa ggttaattta
     2221 ccgtctctga aggcccggct tgggccaagg ggacggaaat gcggccaacc aacaagacgc
     2281 tgcgcgctgc gctcttcggc acgggagcgt tgcttgccac tgcgagcgtc tacgcgcaga
     2341 tcaacgatcg gattcgcggc gcgccgagcg aggcgaatca tttcgtcgag acgccgcgcg
     2401 gctgggtgca tccgaagacg ccgtggggcg agcccgacat ccaggccaag ctcaacatga
     2461 tgcaggcggc cggcgtgccg ctcgagcgct gcgcgaacag ctatcgcgcc gcgctgcctc
     2521 cgggcgcggg tgcgccgccc ggcccggcgg gcggtgtcgg cgcgcccgga cagccgtgcg
     2581 acatgaacaa gaaatggctg acggaagagg agtacgcgca gcgcatggag gcgttccgca
     2641 cacgcgtcga cgcgagccgc cagttcgctg agcagggcaa cctgggccgc gcgatgatga
     2701 cggggctcac ggatcccgcc atcccgcagc gccagaccaa tctcatcgtc gatccgccga
     2761 acgggttatt gccgccgctg acggcggaag gcaagcgccg cgcgatgcgc gcgaagagcg
     2821 actgggcgct gccgggtgag acgatcgctt tcgaaggccc ggagagcttc gatagctggg
     2881 accgctgcat cacgcgcggc atgccgtcgt cgatgatgcc gtatcggtac aacggcgggt
     2941 tctcgattcg ccaagcgccg ggctacgtga tcttcgatct cgaaatgatt cacgaatcgc
     3001 gcgtcattcc gacgaacggc gggcagcacc tgccgccgga ggtcaagcaa tggatgggtg
     3061 actcgcgcgg ccactgggaa ggcaacacgc tcgtcgtcga gacgacgaat tatcaggaag
     3121 gtgccccgat catcaatctc gccgtcgtcg gctcgccgcc cggcaatcgg ttcccgtaca
     3181 gcgaccagat gaagacgaca gagcgcatcg ttcgtctgaa cgacaagacg tggctctacg
     3241 agatcaagac cgaggatccc gtgattctca cggcgccgtt cacggtccgt tatccgatga
     3301 accacgacga cagctattgg tggtcggagt acgcgtgcca cgaggacaac acgatcgtcc
     3361 gcaactacac ggtcacgagc cggcacgagc gcgcgaatcc gacgcccgag ccgccgcagg
     3421 cgcccgtgca agtcgcgccc ggccttgccg acgcgctcgc tggccgctgg gtcggccgtc
     3481 cgcgcatcgt gacgatcgac gtcgacatcg agctcgaatt cacgaagaac gccgacggca
     3541 ccgtcaacgg caagctgatc ggcacgaact taggtcccat cgatcgcccg ctgcgagggt
     3601 tcacgatcgt ggacaacacc gtttacttca cgctgccgaa cgtcgatccg tggcggtttt
     3661 cgggcgagct cggcgacgga accatcaccg gtgtcgtcgc gagcgcgcag gggggcgtgc
     3721 ccgtgacatt ccgcaagcag ccgagtcgag cgaggtaaca acaatgttga agatgacggg
     3781 ccgcatcggc ggcctactgc taggtgcgat cgcgatgccc gcgttcgcgt gcgatttgcc
     3841 gaaagtgccg ctgataccgg agaaggacaa ggtcggcgat caggcgccga cgatcagcgc
     3901 cgcgacgggc gcctacttcg acggcatgcg ggcgtacacc gattgcaccg aggccctgct
     3961 cgatgcggcc ggtggcgagg ccgcgtcgcc gtcgttcaag ggtgtcatca acgggcggct
     4021 caccgttgcc gtcgccgaag cgcaagcggt gcagaagctg ttccaggaac gcgtcggggc
     4081 cggtcaaacg gcgcaagccg gcagcgaggc cgcgctgcgc aagctgatcg aaggcctcgc
     4141 cagcggcaag ccggactacg acgcgatgac gccggaattt gccaaacaaa cgcgacagat
     4201 gatccggttc caacagccca tgatcgcggc cgcgggtgcg atcaagagca tcgagttcgc
     4261 cggcatcgac accgaaggcc gcaacatctt ccaggtcacg caagagaagt ccatgacgaa
     4321 cgcgcgaatc gcgctcgacg cggacggcaa gatcgaattc gcgctcttgc ggcccgcgcc
     4381 gacgccgggc gagaagcggc cgacggcgac gataccgacg cgctccagcg gtctcaaacg
     4441 cgaccgctag cgcgcgcggt gtccgcagtc tagcgccgcc cgtcagcgcg cgcggtagcc
     4501 cgtcgtcgtt tgccagtcga tgtcctcgaa gcgcgccgcg cggcgcagct cgctccaata
     4561 tcgcgacgtg agccgccgcg cctcgctgtc gccgggcggg cccgacagcc cgtgcgtgcg
     4621 ccagttgatg tcctcgaagc gcgccgcttg gcggatctcg ctccacacgc ggctcaggtc
     4681 cgcggcttct tcgcgtgtga acgggctgtc gttgtcgtcg cgctccgtgc cgggaaagcc
     4741 gcgctcgagt cggccgtagc gctcggcgcg gtccgaccgc gatgaataac gactgtcgta
     4801 gtagcggtcc cagtcgatgt cctcgaagcg ctcttcacgc cggatctcgt cccagttgga
     4861 agagagcaag cggcgcgctt cggcgctgcc gggcgcgcgt tctaagccgt gcctcggcca
     4921 agtgatgtcc tcgaagcgtg ccgctacacg gatctgtggc cagactctgg ccaacgcgtc
     4981 agcctcgtcg ctcgtgaaag gaccctcgag ccggccgtaa cgatcgccgc tccggctcga
     5041 ttgcgagtag cggttgttcg cgtactcatc ccagtcgatg tcctcgaagc gctcttcgcg
     5101 ccggatctcg ttccagttcg cggagagcaa gcgctgcgcc tccgggctgc ccggcgcgcg
     5161 cgtgaggccg tgcgtcggcc agttgatgtc ctcgaaacgc gcagccacgc ggatttccgg
     5221 ccagacgtcg ctcaacgcct cggcctcgta gcccgtgaac gggccggtgg acccacggtt
     5281 ttgcgcgtgc accgccgtcc ctgcgatgag cgccacgaga agtgctgtcg cgagtttcat
     5341 ttcgcctcct tgcatgcatg cgttcgggag ccctgttacg agcaagacgc gtgccagcgg
     5401 cggagcgaaa acagctcagt tgatctggta gatacggacc ttttcgttgc tggtttcgat
     5461 cttggtgacg ttggccgttt ccatgttcac catgaccatg agctcggcgg gccgcgacgg
     5521 gcggccgagc agccggtcga gctcgggcgc gttgtagatg tcgacggcgg gcgcgtcgtc
     5581 ggcgacgagc accggcggcg cgtcgctcgg ctttcgcgtg agaacaatgg ccgtgacgag
     5641 cgcgacggcc gcgacggcgg ccgccgccgc gagcacgcga gcgcgccgcg aaggccgttc
     5701 ggccgcgatt tcgtcgagcc gccgcgcgaa cgcttccgcg ccgccgggcg gcggctcgag
     5761 ttgcctgaac agttcgtcag tattcatgtg aaactacccc ttgcacgtcg agccgctcgc
     5821 gcagcaatgc aagcgcgcgc gtcctgcgcg agccgacggt gccttcggcg atgccgagcg
     5881 ccgcggcaat ctcaggatac gtcatgccgg caagctcggt gagcaacagc acgtcgcgca
     5941 gcgtcgtcgg caaattgtcg aacgcttcgc ggaccggttt cgagaagaca tccgtcgaat
     6001 cgctttcgtc ggcgacgtcg tcgagcgctt cgacgccgac gacgcgccag acgcgcgtgc
     6061 ggcggcgccg gttgctcgcg agattcaacg ccgtgcgaaa gagcagcgcc ttgaatccgt
     6121 cggcgcggat gtcgtccttg cggcgccagc agcgcaggaa cgtttcctgc acgacgtcct
     6181 gactctccgc ggcatcccag atgactcggt acacgacgtt gtacagcggc ttctccaagc
     6241 gccggtacac gtcaacccac tgagacttgt cggggctcgg catgtcgagc cgtgtgttgc
//
LOCUS       DASSNN010000685         3771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_856871, whole genome shotgun sequence.
ACCESSION   DASSNN010000685 DASSNN010000000
VERSION     DASSNN010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856871"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFL84_12855"
     CDS             complement(<1..462)
                     /locus_tag="VFL84_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010672428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7609563.1"
                     /translation="MDNGFYQRLDSELTELRAQGLFKSERVISSPQGGQVRANDADVI
                     NLCANNYLGLANHPAIREAAHRALDRYGYGMASVRFICGTQAVHKQLEAAMSRFLGTE
                     DTILYSSCWDANGGLFETLLGEQDAVISDSLNHASIIDGIRLCKARRLRYAN"
     gene            634..2502
                     /locus_tag="VFL84_12860"
     CDS             634..2502
                     /locus_tag="VFL84_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609564.1"
                     /translation="MGDAEERESVDARSADRSDSFWARVQEHKIIQWGLGYLGAAFAL
                     AQGAELVGNAFDWPDVVGRILILALIAGLPVALALAWFHGHRGQQRVSTGELWVISAL
                     VLLGGIFVTVAFRETDDAAANAATSATEASPSGIAAPRNAEAREPNSIAVLPFANISS
                     DQEQEYFVDGLSEELMNQLAQVRALRVTARTSAFAFKGSAETVDKIGDQLKVAHVLEG
                     SVRKAGSQLVITATLIDTATGFNTWSKSYDRELKDIFAIQQDIAREVVAALKVTFGID
                     EDQRIPGGTDNLDAYAMYLQATSRPQASIPAAQAALNLTEEALKLDPNFSLAWAHKAR
                     LLLFLRLDPRRSGPELQAMAEEAAQRAVELAPDSPVAHAVMANAASVRGDWRTAEAKY
                     RQVLARGDPPERGGGYGLLQLAVGHVYAARDRLLLQRQRDPLNEAAVAFLVAAEDSLG
                     NTQAAGAEYERGAQLFRPWFAGWYNGVLTLAGIPEANFRDAPASVGFSFADPPFAAIV
                     ASWDDPAAARQAVRAAYASIDQSVVPLWRVLARLQIGALGARFGDPELAMTSFEETFG
                     SSPEQVYAIWRPVYRDMRKLQRFKEFVRQIGLVDYWKQYGWPDVCRPFGENDFECD"
     gene            2547..3299
                     /locus_tag="VFL84_12865"
     CDS             2547..3299
                     /locus_tag="VFL84_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7609565.1"
                     /translation="MRTQQRLLPWLALALTAVAAQAAHAQDEQVARMRAALAAPERPA
                     ENKARDAARKPIETVQFFGIKTGQTAIDLIAVGGWFTEVLSAAVGPTGKVYSQNPPFL
                     VNADAEKALLARLGNAQAVHDRLDQSIPAGSVDAAVTAMNLHDIYNGYADQAGGEQPA
                     VDFLKAVYATLKPGGVLGVIDHVGVAGKDNVMLHRMLPQQARDAITKAGFTIEAESTL
                     LANPADDHTKNVFDPSVMGHTDQFVFRARKPR"
     gene            complement(3346..>3771)
                     /locus_tag="VFL84_12870"
     CDS             complement(3346..>3771)
                     /locus_tag="VFL84_12870"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7609566.1"
                     /translation="VLELQAVCDAARRYIDEGGVADRVDTLSIDMFRDAWPRGYDGVF
                     FSNIFHDWNADTNVFLARRAYEALEPGGRILLHEQLLAEDGSGPVTTAAFSMLMLLGT
                     HGRQYTFGELKQILSSAGFVDVDSCATYGYYSVVSGRKA"
BASE COUNT          647 a         1240 c         1273 g          611 t
ORIGIN      
        1 gttcgcgtaa cgcaggcggc gcgctttgca gagccgaatg ccgtcgatga tgctcgcgtg
       61 attcagcgag tcggagatca cggcgtcctg ctcgccgagc agcgtctcga acaagccgcc
      121 gttcgcgtcc cagcaggacg aatagaggat cgtgtcctcg gtgccgagga accgcgacat
      181 cgccgcctcg agctgcttgt gcacggcttg cgtgccgcag atgaagcgga ccgacgccat
      241 gccgtagccg tagcggtcga gcgcgcggtg cgcggcttcg cgaatcgcgg gatggttcgc
      301 gagcccgagg tagttgttcg cgcacagatt gatgacgtcc gcgtcgttcg cgcggacttg
      361 cccgccctgc gggctcgaga tcactcgctc gctcttgaag agtccttgcg cgcgcagctc
      421 cgtgagctcg gagtcgagtc gctgatagaa gccgttgtcc acgcgtgacc ccgattacag
      481 gagctgggcg caattatacg tgcgcccccc agccggcccc caaaatgggg acagatctat
      541 tttcggcgaa aatagatctg tccccttttg ccggggccgg agtgctacca tcggcggcgc
      601 tgcatagcgg cacgcctgcg agagagcatc tctatggggg acgcggaaga gcgcgagtca
      661 gtcgacgcga ggagcgccga tcgctcggac agcttttggg cacgcgtcca agagcacaag
      721 atcatccagt ggggcctcgg ctatctcggc gcggcgttcg cgctcgcgca gggtgccgag
      781 ctcgtcggca acgcgttcga ttggccggac gtcgtcggcc ggatcctgat cctcgcgctc
      841 attgcgggct tacccgtcgc gctcgcgctc gcgtggttcc acgggcatcg cggccagcag
      901 cgtgtgagca ccggagagct ctgggtcatt tccgctctgg tcttgctcgg cggcatattc
      961 gtgacggtcg ctttccggga gacggacgac gcggctgcga acgccgcgac gagcgcgacc
     1021 gaggcctcgc cgtccggcat tgccgcgcca cggaacgcgg aggctcgcga gccgaattca
     1081 atcgcggtgc tgccgttcgc gaacatctcg tccgatcagg agcaggagta tttcgtcgac
     1141 gggctttccg aggagctgat gaatcagctc gcgcaagttc gagcgctgcg cgtgacggcg
     1201 cgcacgtcgg cattcgcgtt caaaggctcg gcggaaaccg tcgacaagat cggcgaccag
     1261 ctgaaagtcg cgcatgttct cgaaggcagc gtacgaaagg ccggcagcca gctcgtgatc
     1321 acggcgacgc tgatcgacac ggcgacggga ttcaacacct ggtcgaagtc ttacgaccgc
     1381 gagctcaagg acatcttcgc gatccagcaa gacatcgcgc gcgaggtcgt ggccgcgctg
     1441 aaggtcactt tcggcatcga cgaagatcag cggatcccgg gcggcaccga caacctcgac
     1501 gcctacgcga tgtacttgca ggcaacgtcg cggccgcagg ccagcatccc ggcggcccag
     1561 gccgcgctga atctcaccga ggaggcgctg aagctcgacc cgaatttttc gctcgcatgg
     1621 gcgcacaagg cgcggctgct gctcttcttg cggctcgatc cgcgccgcag cggtcccgag
     1681 ctccaggcga tggccgagga ggccgcgcaa cgcgcggtcg agctcgcgcc ggattcgccc
     1741 gtcgctcacg ccgtgatggc caacgcggcc agcgtacgcg gcgattggcg cacggccgag
     1801 gccaagtatc gacaagtgct cgcgcgcggc gaccctccgg agcgcggcgg cgggtacggt
     1861 cttctgcagc tcgcggtcgg ccatgtctac gcggcgcgcg accggctgct tttgcaacgg
     1921 cagcgcgacc cgctgaacga ggcggccgtc gccttcctcg tcgccgccga ggacagtctc
     1981 ggcaacacgc aggccgccgg ggccgaatac gagcgtgggg cgcagctgtt ccgcccgtgg
     2041 ttcgccggtt ggtacaacgg agtcttgacg ctggccggaa ttcccgaggc gaacttccgc
     2101 gacgcgccgg cttcggttgg cttctcgttc gccgatccgc cgttcgccgc gatcgtcgcg
     2161 agctgggacg atcccgccgc ggcgcggcag gcggtccgtg ccgcctacgc gtcgatcgac
     2221 cagtcggtcg tgccgctttg gcgcgtgctc gctcgcctgc aaatcggcgc gctcggcgcg
     2281 cgattcggcg atcccgagct cgcaatgacg tcgttcgagg aaaccttcgg gtcgagcccc
     2341 gagcaggtct acgcgatctg gcgtcccgtc tatcgcgaca tgcgcaagct tcagcgcttc
     2401 aaggaattcg tgcggcagat cggtctcgtc gattactgga agcagtacgg ctggcccgac
     2461 gtgtgccgcc ccttcggcga gaacgatttc gaatgcgatt gacagcgccg gcgcgtccgt
     2521 cggtgtaaaa cacaaggagt attccgatga gaacgcagca acgattgcta ccgtggctcg
     2581 cattggcgct gaccgccgtc gccgcgcaag ccgcgcacgc gcaagacgag caggtggcac
     2641 gcatgcgggc ggcgctcgcg gcgcccgagc ggccggccga gaacaaagcg cgcgacgcgg
     2701 cgcgcaagcc gatcgagacc gtgcagttct tcgggatcaa gacgggccag accgcgatcg
     2761 acttgatcgc cgtcggcggt tggttcacgg aggtgctgtc ggccgccgtg ggcccgacgg
     2821 gcaaggtgta ttcgcagaac ccgccgttcc tcgtcaacgc cgacgccgag aaggcattgc
     2881 tcgctcggct cggcaacgca caggccgtgc acgatcggct cgatcagtcc attccggcag
     2941 gcagcgtcga tgccgcggtg acggcgatga atttgcacga catctacaac ggctatgcgg
     3001 accaagcggg cggcgagcag ccggccgtgg actttctgaa agcggtctac gcgacgctga
     3061 agcccggcgg cgtgctcggc gtgatcgatc acgtcggcgt cgccggcaag gacaacgtga
     3121 tgctgcatcg catgctgccg cagcaggcgc gcgacgcgat cacgaaggcc ggcttcacga
     3181 tcgaggccga atcgacgctg cttgcgaacc cggccgacga tcacacgaag aacgtgttcg
     3241 acccgtcggt catggggcac acggaccaat tcgtgttccg cgcgcgcaaa ccgcgctaga
     3301 acgaaaggac gccaccacct gcgcgagcgg ccgccgccag caggcctagg cctttcggcc
     3361 gctcacgacc gagtagtacc cgtaggtcgc gcacgaatcg acgtcgacga accccgcgct
     3421 cgagagaatc tgcttgagct cgccgaacgt gtactggcgg ccgtgcgtgc cgagcagcat
     3481 caacatcgag aatgccgcgg tcgtgaccgg ccccgagccg tcctcggcga gcagttgctc
     3541 gtgcagcaag atgcgcccgc ccggctcgag cgcttcgtac gcgcgccgcg cgagaaacac
     3601 gttcgtgtcg gcgttccaat cgtggaagat gttcgagaag aacacgccgt cgtagccgcg
     3661 cggccacgcg tcacggaaca tgtcgataga taaagtatcc acgcgatccg cgacgccgcc
     3721 ttcgtcgatg tagcggcgcg cggcgtcgca gacggcctga agctcgagca c
//
LOCUS       DASSNN010000686         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_621079, whole genome shotgun sequence.
ACCESSION   DASSNN010000686 DASSNN010000000
VERSION     DASSNN010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621079"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VFL84_12875"
     CDS             <1..1125
                     /locus_tag="VFL84_12875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7609567.1"
                     /translation="LALAACAVGEAGNPPPAPPAPQVTTAEVAVRELNDWADFTGRLE
                     AVESVEIRARVGGYVESVHFAEGGRVEQGALLYQIDPRPFKAEVDRLTAERARAAAQL
                     DLAQSYRDRAERLLARNATSQEEFEQHAADATVAAAQLASIGAALEKAELDLEFTRVT
                     APIAGRVSRAIVTAGNLVDAQTVLTTLVSDDAVYAYFDVDEHTYLEHVRQASAPEASV
                     VHIGLVNEQGYPHTARLDFVDNQVDPSHGTIRARAVLDNRDGELTPGLFARMQLVSPT
                     RYSAALVDDRAVGTDLGRRFVFVVDDQGVVQYRPVETGRLVDGLRVVTNGLAAGDVVI
                     VNGVQRVRPGVSVAETRVAMGHDLPPLARAADAAVANAAY"
     gene            1151..>2334
                     /locus_tag="VFL84_12880"
     CDS             1151..>2334
                     /locus_tag="VFL84_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019409684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7609568.1"
                     /translation="MRFSQFFIQRPIFAGVLSAIIFIAGALSLELLPISEYPEVVPPT
                     VVVRAIYPGANPQVIAETVASPLEQAINGVEDMLYTSSQSTADGLMTLNVTFELGTDL
                     DQAQVLVQNRVAQALPKLPEVTQRLGVVTEKSLPDFTLVVHLVSPNDRYDMLYLSNYA
                     TLRVRDELKRIDGVGDAQVFGAGEYSMRVWLDPERVAALNLTASDVVRAIREQNVQVA
                     AGQLGSPPAPSAAQFQLLVDTQGRLVSEEEFEDIVVKTGDRGQITRLKDVARVELGAS
                     TYALRSLLDNEPAVAIPVYQQPGSSNAIEISNKVRAKMEELKKEFPEGVDYRIVYDPT
                     VFVRESIGAVVQTLVEALVLVVLVVVLFLRTWRASIIPLAAVPVSLVGTLAAMHLLGF
                     SL"
BASE COUNT          363 a          825 c          780 g          366 t
ORIGIN      
        1 ctcgcgcttg ctgcttgcgc cgtcggcgaa gccggtaacc ccccaccggc tccgccggcg
       61 ccgcaagtga cgacggccga ggtcgccgtg cgcgagctca acgactgggc cgacttcacg
      121 ggccgtctcg aagccgtcga aagcgtcgag atccgcgccc gagtcggcgg ctacgtcgag
      181 tccgtgcact tcgccgaagg cgggcgcgtc gagcaaggcg cgctcttgta tcaaatcgat
      241 ccgcggccgt tcaaggcgga agtcgatcgt cttacggccg agcgcgcgcg cgcggcagcg
      301 cagctcgacc tcgcacagtc gtatcgcgat cgcgccgagc gcctgctcgc gcgcaacgcg
      361 acgtcgcaag aagagttcga gcagcacgcc gccgacgcaa cggtcgcggc cgcgcagctc
      421 gcctcgatcg gcgccgcgct cgagaaggcc gagctcgacc tcgagttcac gcgcgtgacg
      481 gcgccgatcg cggggcgcgt ctcgcgcgcg atcgtgacgg ccggcaatct cgtcgacgcg
      541 caaacggtgc tgacgacgct cgtctccgac gacgccgtct acgcgtactt cgacgtcgac
      601 gagcacacat atctcgagca cgtgcggcag gccagtgcgc ccgaggccag cgtcgtgcac
      661 atcggcctcg tcaacgagca aggctacccg cacacggcgc ggctcgactt cgtcgacaac
      721 caagtcgacc cgagccacgg cacgatccgt gcgcgcgccg tgctcgacaa ccgcgacggc
      781 gagctcacgc cgggcttgtt cgcgcgcatg cagctcgtga gcccgacgcg ttacagcgcc
      841 gcgctcgtcg acgaccgcgc ggtcggcacc gatctcggca ggcgcttcgt gttcgtcgtc
      901 gacgaccagg gcgtcgtgca gtaccgcccc gtcgagaccg gtcggctcgt cgatggcctg
      961 cgcgtcgtga cgaacggcct cgccgccggc gacgtcgtca tcgtcaacgg cgtgcagcgc
     1021 gtgcgcccgg gcgtcagcgt cgccgagacg cgcgtcgcga tgggtcacga cctgccgccg
     1081 ctcgcgcgcg cggccgatgc cgctgtcgcg aacgcggcgt actaacgcgg ctttccaagg
     1141 gagcaaagcc atgcgcttct cgcaattctt catccagcgg ccgattttcg ccggcgttct
     1201 gtcggcgatc attttcatcg ccggcgcgct gtcactggag ctcttgccca tcagcgaata
     1261 ccccgaggtc gtgccgccga cggtcgtcgt gcgcgcgatc tatccgggcg ccaacccgca
     1321 agtcatcgcg gagaccgtcg cgtcgccgct cgagcaggcc atcaacggcg tcgaggacat
     1381 gctctacacg tcgtcgcagt cgacggccga cggcctcatg acgctgaacg tcacgttcga
     1441 gctcggcacc gacctcgatc aggcgcaggt gctcgtgcag aaccgcgtcg cgcaggcgct
     1501 gccgaagctg cccgaggtca cgcagcggct cggcgtcgtg acggagaagt cgctgccgga
     1561 cttcacgctc gtcgtgcacc tggtgtcgcc gaacgaccgc tacgacatgc tgtatctgtc
     1621 gaattacgcg acgctgcgcg tgcgcgacga gctcaagcgc atcgacggcg tcggcgatgc
     1681 tcaggtgttc ggcgccggcg agtattcgat gcgcgtctgg ctcgacccgg agagggtcgc
     1741 cgcgctgaac ctcacggcca gcgacgtcgt gcgcgcgatc cgcgagcaga acgtgcaagt
     1801 cgcggcgggc cagcttggga gcccgcccgc gccgagcgcg gcgcagttcc agcttttggt
     1861 cgacacgcaa ggccgtctcg tcagcgagga agagttcgag gacatcgtcg tcaagaccgg
     1921 cgaccgcggc cagatcacgc gcttgaagga cgtggcgcgc gtcgagctcg gcgcgagcac
     1981 gtacgctctg cgctcgctgc tcgacaacga gcccgccgtc gcgattcccg tgtatcagca
     2041 gcccggcagc agcaacgcga tcgagatctc gaacaaggtc cgcgccaaga tggaggagct
     2101 caagaaggag ttcccggaag gcgtcgacta ccgaatcgtt tacgacccga ccgtgttcgt
     2161 gcgcgaatcc atcggcgcgg tcgtgcagac gctcgtcgag gcgctcgtgc tcgtcgtgct
     2221 cgtcgtcgtg ctgttcctgc gcacgtggcg cgcgtcgatc attccgctcg cggccgtgcc
     2281 cgtgtcgctc gtcggcacgc tggccgcgat gcatttgctc ggcttctcgc tgaa
//
LOCUS       DASSNN010000687         4994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1247815, whole genome shotgun sequence.
ACCESSION   DASSNN010000687 DASSNN010000000
VERSION     DASSNN010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247815"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VFL84_12885"
     CDS             <1..700
                     /locus_tag="VFL84_12885"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET7609569.1"
                     /translation="NILGPIHVTGHDASRVEMHATETVRGDLQADIERARAQMQLRTE
                     TEPGRVAIRVRHVGDDDDGRRNSGWDNYSIEYDIDVRVPRGATLHVSTVNDGDVTVED
                     VNGEFELKNVNGGVRLVRAGGNGTINTVNGDVEAIFARAPSAATSFHTVNGDLDVTFP
                     ANLAAELAFHTMQGDVFTDFDVESLSVPPEVRRDRGRYVVNTNRNSAFRVGAGGERHS
                     FNTLNGDIFVRKAK"
     gene            701..1522
                     /locus_tag="VFL84_12890"
     CDS             701..1522
                     /locus_tag="VFL84_12890"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET7609570.1"
                     /translation="MSNRIGIFALLTGASCAVSGLAAAQDDQRLVVPLTDPSRPGRLE
                     INLFSGDISVEGYDGKEIVIVADAPIRDADQPEPPRQDGLRRIQSSSVGLTVEENDNT
                     VLVRMDFSPKNADLQIRVPRRTSVKASLVNGGDVEIDSVTGDHELQNVNGDVIATDIA
                     GSAVINATNGDVRATFTSVEAMKPMSFTSFNGDVDVTLPANIKADLIVGSQQGDVYTD
                     FDVTAQTDPPNVQQRVGPRGGREVRVSHQTRYAVGGGGSDIEMRTFNGDVMIRKR"
     gene            1615..1863
                     /locus_tag="VFL84_12895"
     CDS             1615..1863
                     /locus_tag="VFL84_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609571.1"
                     /translation="MNDDRIASLLGEIRDNQRQALEQQRAQLEIAHSHFEQAKAQIAE
                     SLKLQRVAISRQRAALWIALPALFLCIAAIVYLIWRYF"
     gene            complement(1866..2672)
                     /locus_tag="VFL84_12900"
     CDS             complement(1866..2672)
                     /locus_tag="VFL84_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609572.1"
                     /translation="MRIGETRALGLKSAVAVAALFAAGAAAAQSAGAVPRASDGHPDL
                     SGTWDNGAGIDFVQPRKDGKSICIFGCPGGAPPPESVPQASVPKYRPEFAARVADLDA
                     RQVQEDPVLRCFAPGVPRIGPPDKIVQRAGEAVFLYEDVSGSFFRLVPTDGRPHPTNN
                     EPSFLGDAVGHWEGDTLVVETKNFNDQSWLTDNGAFHTDALRVVERLSRTADTLEWTA
                     TAYDPAVLAEPYTTAKRVAKLTDLEIVESPPCIDRSLANMVDSSHHTNPR"
     gene            complement(2685..3077)
                     /locus_tag="VFL84_12905"
     CDS             complement(2685..3077)
                     /locus_tag="VFL84_12905"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609573.1"
                     /translation="MARTVLPLVLVLGALSFYGGTAAAHHSVAGQFDQAQRATITGVI
                     SKVEWINPHVYIQMDVPDEKGAITTWRLESLPTAMLRKAGLTSEMLKGGGQKVTAAVL
                     LARNGTPNLGWLLNLSYEDGHEYVLAGE"
     gene            3294..4355
                     /locus_tag="VFL84_12910"
     CDS             3294..4355
                     /locus_tag="VFL84_12910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7609574.1"
                     /translation="MCTDVGRAVLELADEAAPQQVANFRRYVDMGFYSGTVFHRVQPG
                     LFVQGGGMDRELRVRSTLPPVPNESSNGLHNTRGTVAAARTEDPDSARSQFFVNLADN
                     TDELEGGRAGYTVFANVTEGIDVFEAIGRLPTGPAGPFRADVPTPLVAIKSIARLDAA
                     ALAALPEDGREAALKERIMAAAAAGSTIDALDAIGQYRSLCGADDPEIALTEARMALA
                     LDDRRRAMFVLEELMATTEPEEPAHAAAMELYREAATDGAMTSQLLAACAPPAVPALP
                     DARSVSEAEMIASQRQVREFVAGGDAYLACLDRVINDEDRAASLRNAAVEEYNRMVAA
                     MEEIAAGFNEQIRIFKARG"
     gene            complement(4339..>4994)
                     /locus_tag="VFL84_12915"
     CDS             complement(4339..>4994)
                     /locus_tag="VFL84_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HET7609575.1"
                     /translation="NIVVEIPNLEPRAIERAVGIDLGLKDLAALSTGEKIENPKQLAK
                     LAGKLALAQRANKKRLATNIHARIKNARNDFLHKASHRIVRDFDYIAVGNVSAAGLVK
                     TRLAKSVLDAGWSSFRNMLRYKAIARGAWYEEVNERFSTQVCSACGSLPEQRPKGIAD
                     LGVREWTCGDCGSVHDRDQNAASNILRVGHPSPAVGTSGPPGRGACQAAILPLSRAP"
BASE COUNT          850 a         1657 c         1658 g          829 t
ORIGIN      
        1 gaacatcctc gggccgattc acgtcacggg ccacgacgcg agtagagtcg agatgcacgc
       61 gaccgaaacc gtgcgcggcg atttgcaggc cgacatcgag cgcgctcgcg cgcagatgca
      121 gctgcgcacg gagaccgagc ccggacgcgt cgcgatccgc gtgcgccacg tcggcgatga
      181 cgacgacggc cgccgcaact cgggctggga caactacagc atcgagtacg acatcgacgt
      241 gcgcgtgccg cgcggcgcca cgcttcacgt ctcgacggtc aacgacggcg acgtgaccgt
      301 cgaggacgtc aacggcgagt tcgagctcaa gaacgtcaat ggcggcgtgc ggctcgttcg
      361 ggccggcggc aacggcacga tcaacaccgt caacggcgac gtcgaagcga tattcgcgcg
      421 cgcaccgagc gccgcgacgt cgtttcatac cgtgaacggc gatctcgacg tgacgtttcc
      481 ggcaaacctc gcggccgagc tcgcgttcca cacgatgcag ggcgacgtct tcacggattt
      541 cgacgtggag tctttgagcg tgccgcccga ggtgcggcgc gaccgcggcc ggtacgtcgt
      601 gaacactaac cgcaactccg cgttccgcgt gggcgcgggc ggggaaagac attccttcaa
      661 cactttgaac ggcgacattt tcgtacggaa ggcgaaataa atgagcaaca gaataggcat
      721 ctttgcattg ttgacgggcg cttcgtgcgc ggtctcgggc ctcgccgcgg cgcaggacga
      781 ccagcgcctc gtcgttccgc tcacggatcc gtcgcggcct gggcggctcg agatcaattt
      841 gttctccggc gacatcagcg tcgaaggtta cgacggcaag gagatcgtca tcgtcgccga
      901 cgcgccgatc cgcgacgcgg atcagccgga gccgccgcgg caagacggtc tgcgcaggat
      961 tcagagctcg tccgtcggcc tcacggtcga ggagaacgac aacaccgtcc tggtccgcat
     1021 ggacttcagc cccaagaacg ccgacctgca gattcgcgtc ccgcggcgga cgtccgtcaa
     1081 ggcgagcctc gtcaacggcg gcgacgtcga gatcgactcc gtgacgggcg accatgagct
     1141 tcagaacgtc aacggcgacg tcatcgcgac cgacattgcg ggctcggccg tcatcaacgc
     1201 gacgaacggc gacgtgcgcg cgactttcac gtcggtcgag gcgatgaagc cgatgtcgtt
     1261 cacgtcgttc aacggcgacg tcgacgtgac gctgcccgcc aacatcaagg ccgacttgat
     1321 cgtcggcagc cagcagggcg acgtgtacac ggacttcgac gtcaccgcgc agaccgatcc
     1381 gccgaacgtc cagcaacgag tcggcccaag gggcggcagg gaagtccgcg tgtcgcacca
     1441 gacgcgttat gcggtcggcg gcggcggctc ggacatcgag atgcgcacgt tcaacggcga
     1501 cgtgatgatc cgcaaacgtt agacggcgat cgacgttttt gcgacgcgct acggggtagg
     1561 cgtcgaccgt ttcggcatac cgtgggagct caggagcccc acggaagcgc gaggatgaac
     1621 gacgatcgaa tcgcgagctt gctcggcgag attcgcgaca accagcgcca ggcgctcgag
     1681 caacaacgcg cgcagcttga gatcgcgcac agtcatttcg agcaggcgaa agctcagatc
     1741 gccgaatcgc tgaagctgca gcgcgtagcc atcagccgcc agcgcgcagc attgtggatc
     1801 gccttgccgg cgctctttct ctgcatcgcc gcgatcgtct atctgatctg gcgctatttc
     1861 taagatcagc gcgggttcgt gtggtgcgag ctgtcgacca tgttcgcgag ggaacggtcg
     1921 atgcacggcg gcgattcaac gatctcgagg tccgtgagct tcgcgacgcg cttcgccgtc
     1981 gtgtacggct ccgcgagcac ggcgggatcg tacgccgtcg ccgtccactc gagcgtgtcg
     2041 gccgtgcggc tcagccgctc gacgacgcgc agagcatccg tgtggaacgc gccgttgtcg
     2101 gtcagccacg attgatcgtt gaagtttttc gtctccacga cgagcgtgtc gccttcccaa
     2161 tggccgaccg cgtcgcccaa gaagctcggc tcgttgttcg tcgggtgcgg ccggccgtcg
     2221 gtcggcacga gccggaagaa gcttcccgag acgtcctcgt agaggaacac ggcttcaccc
     2281 gcgcgctgga cgatcttgtc cggcgggccg atgcggggca cgccgggcgc aaagcagcgc
     2341 agcacgggat cctcttggac ttgccgcgcg tccaagtcgg cgacgcgcgc ggcgaattcc
     2401 ggccggtact tcggcacact cgcctgaggg accgattcgg gcggaggcgc gccgcccggg
     2461 cagccgaaga tgcagatcga cttgccgtcc ttgcgcggct gcacgaagtc gatgcccgcg
     2521 ccgttgtccc aggtgccgct caggtccgga tgaccgtccg atgcgcgcgg taccgcgccc
     2581 gccgactgcg ccgcggcggc gccggcggcg aacagcgccg cgaccgcgac ggcggactta
     2641 agccccaatg ctcgtgtttc gccaatgcgc atcttgagcc tccgtcattc gcccgcgagc
     2701 acgtactcgt ggccgtcttc gtaggagaga ttcagcagcc atcccaagtt aggcgtgccg
     2761 ttgcgtgcga gcagcacggc cgcggtcact ttctggccgc cgcccttcag catctcgctc
     2821 gtgaggccgg ccttgcgcaa catcgcggtc ggcagggatt cgagccgcca cgtcgtgatc
     2881 gcgcccttct cgtcggggac gtccatctgg atgtagacgt gcgggttgat ccactcgact
     2941 ttgctgatga cgcccgtgat cgtcgcgcgc tgcgcctggt cgaactgacc cgcgacggaa
     3001 tgatgcgcgg ccgcggtgcc gccgtaaaac gagagcgcgc cgagaacgag caccagcggc
     3061 aggacggttc gtgccattgc agtcacccca caccccgagc ggcagccaag atcttaacgc
     3121 agttgcttgc gctgtcgctc gccggcgtca ccggctgggc tcgttcggcg gggtgctatg
     3181 ctcggcggcg ccttacggtt gagggatcga ggccgatgag tcttcgcttg cgggcgctct
     3241 gcgcgctcgg cgcactgtgc ggcgccggaa cactgtctgc gaccgaggtc gccatgtgca
     3301 cggacgtggg gcgcgccgtg ctcgagctcg ccgacgaggc cgcgccgcag caggtcgcga
     3361 acttccgtcg ctacgtcgac atgggcttct actccggcac ggtgttccac cgcgtgcagc
     3421 ccggcctctt cgtccaaggc ggcggcatgg accgcgagct gcgcgtgcgc tcgacgcttc
     3481 cgcccgtccc gaacgagtcg agcaacgggt tgcacaacac gcgcggcacc gtggccgccg
     3541 ctcgcaccga ggatcccgat tctgcgcgct cgcagttctt cgtaaatctc gccgacaaca
     3601 ctgacgagct cgagggcggg cgtgccggct acaccgtgtt cgcgaacgtc acggaaggca
     3661 tcgacgtctt cgaggcgatc ggccggctgc cgacgggccc ggcgggacct tttcgcgccg
     3721 acgtgccgac gccgctcgtc gccatcaagt cgatagcgcg gctcgacgcg gccgctctgg
     3781 ccgccctgcc cgaagacggc cgcgaggccg ccctgaaaga gcggatcatg gccgcagcgg
     3841 ccgccggcag cacgatcgac gcgctcgacg cgatcggcca gtaccgctcg ctttgcggcg
     3901 ccgacgaccc ggagatcgcg ttgaccgaag cccgcatggc gctcgcgctc gacgatcggc
     3961 gccgcgcgat gttcgtgctc gaggagctca tggcgacgac ggagccggaa gagcccgccc
     4021 atgccgccgc gatggagctc taccgggaag cggcaaccga cggcgcgatg acgtcgcagc
     4081 tgctcgccgc ctgcgcgccg cccgcggtcc ccgcgctgcc cgacgcgcgc agcgtcagcg
     4141 aagccgagat gatcgcgtcg cagcgccagg tgcgcgagtt cgtggcgggc ggcgatgcct
     4201 atctcgcgtg cctcgaccgc gtcatcaacg acgaggaccg cgcggcgagt ctgcgcaacg
     4261 cggccgtcga agagtacaac cgcatggtgg cggccatgga agagatcgcg gcgggcttca
     4321 acgagcagat ccgcatcttc aaggcgcgcg gctaagcggt aagatcgcgg cttgacatgc
     4381 tccccgccct ggagggccgg aggttcccac agctggcgag ggatgtccca ctcggagaat
     4441 gttcgacgca gcgttttggt cgcgatcatg tacggaaccg cagtcaccac acgtccactc
     4501 tcttactcca aggtctgcga tacctttcgg cctctgctcg ggcagcgagc cgcaagcaga
     4561 acagacctgg gtactgaatc gctcgttcac ttcctcatac caagctccgc gcgcaatcgc
     4621 tttgtagcgc agcatgtttc ggaaggacga ccagccagca tcgagcacgg acttcgccag
     4681 tctggtcttg acgagtcccg cggcgcttac gttgccaacc gcgatgtaat cgaagtctcg
     4741 cacgatgcga tgcgaggctt tgtgcaagaa gtcgttccgc gcattcttga tgcgcgcgtg
     4801 gatgttggta gcgaggcgtt tcttgttggc gcgctgcgcc aaggccaact tgccggcaag
     4861 cttggcgagt tgcttggggt tctcgatctt ctcgccggtc gagagtgccg caagatcctt
     4921 cagtccgaga tcgatgccca ctgcgcgctc gatcgctcgc ggctcaagat tgggtatctc
     4981 gacgacgatg ttca
//
LOCUS       DASSNN010000688         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_493078, whole genome shotgun sequence.
ACCESSION   DASSNN010000688 DASSNN010000000
VERSION     DASSNN010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493078"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VFL84_12920"
     CDS             complement(<1..786)
                     /locus_tag="VFL84_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7609576.1"
                     /translation="MFHPAVAAWFEQTFAKPTEAQARAWPLVAAGRDVLIAAPTGSGK
                     TLAAFLASIDALVREGTKRPLPDETFVVYVSPLKALSNDVRKNLEAPLAGIRAELAAQ
                     GFPDVEIRTLVRTGDTPAHERAAMRKRPPHIVVTTPESLYILLGSASGRRMLETTRSV
                     IVDEIHALAPNKRGSHLALTLARLQALVGHRLVRIGLSATQKPIEEVARFLVGAHGEC
                     EVVDAGHARPRDLALEVPSSPLEAVMSNETWLQVYARLADLVAA"
     gene            944..1603
                     /locus_tag="VFL84_12925"
     CDS             944..1603
                     /locus_tag="VFL84_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609577.1"
                     /translation="MRRALSMSLLVALFASTGALAQGFRFSNPDDSEERDAAARDQRI
                     AEHLAVECRRDLLNKKIMVVIGERQSNGVVEARQENYGPHYQAINRRLQALGLRTYTP
                     EQIKRQIAQAELDAYFRNDPDAALAASRKLGASFVLRGLISAETTRNPMMAVNQVSLN
                     MGFTLTASNGRVISDTQATASSYAGADVRRMAQTLVDEQADEVVAKLYGDYCRNAART
                     R"
     gene            1661..2494
                     /locus_tag="VFL84_12930"
     CDS             1661..2494
                     /locus_tag="VFL84_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609578.1"
                     /translation="MSKRLARLAPPLAGAVIALCSAPLLAQPTFGNPSPTAGSETSQR
                     ANDSADQALYRPVEYVNKARKGPAVIVIPGEIKSNNATFMQKFTANNIADFGELEMSN
                     ANFPVLERSNLGPLLNEFQLAYNLGDPQAARKFLGMGKLKSTKYVVKFDILKAEEVAA
                     AQQGFDGRALGNIAGALGAFSGSWGGARAGAVGSAAVGSVHTGESTGVWIIGMRYKII
                     NAETTEQVAQGYTEEKMEVGAKSSSVLGVSQSQQGGVSLDTMVQRLVQKSVWDIDSKY
                     K"
BASE COUNT          501 a          965 c          987 g          391 t
ORIGIN      
        1 cgcggccacg aggtcggcga ggcgcgcata gacctgcagc caagtctcat tcgacatcac
       61 cgcctcgagc ggcgacgatg gcacttcgag cgcgaggtcg cgcggacgcg cgtgaccggc
      121 gtcgacgacc tcgcattcgc catgggctcc gaccaggaag cgcgcaacct cctcgatcgg
      181 cttctgcgtc gccgacaagc cgatccgcac gagccgatgg ccgaccagcg cctgcaggcg
      241 cgcgagcgtc agggcgagat ggctgccgcg cttgttcggc gcgagcgcgt ggatctcgtc
      301 gacgatgacg ctgcgcgtcg tttcgagcat gcgccggccc gacgccgagc cgagcaggat
      361 gtacagcgat tcaggcgtcg tgacgacgat gtgcggcggc cgcttgcgca tcgcggcgcg
      421 ctcgtgcgcc ggcgtgtcgc cggtgcgcac gagggtgcgg atctcgacgt ccggaaagcc
      481 ttgcgccgcg agctcggcgc ggatgccggc gagcggcgcc tcgaggttct tccgcacgtc
      541 gttcgacagc gccttgagcg gcgacacgta gacgacgaac gtctcgtcgg gcagcggtcg
      601 cttggtgcct tcgcgcacca gcgcgtcgat cgacgcgagg aacgccgcca acgtcttgcc
      661 ggaccctgtc ggtgcggcga tcaggacgtc gcgtccggcc gcgacgagcg gccacgcgcg
      721 cgcctgcgcc tccgtgggct tcgcgaacgt ctgttcaaac caggcggcga cggcgggatg
      781 gaacatgcgg cggtgtcgga ggagtgtcgg aagagcggcg aaacgaccga ttgtcccatt
      841 gttgcatccg ttccgcggcc acccggcggc cggccggccg gagcgatgga tttcgcgcag
      901 cggctcagct aatcttgccg atcgctttcg accgcttacg ccgatgcgtc gcgctctctc
      961 gatgtcgttg ctcgtggcgc ttttcgcaag taccggcgcg ttggcgcagg gcttccgctt
     1021 ctcgaacccg gacgacagcg aagagcgcga cgccgcagcc cgcgaccagc gcatcgccga
     1081 gcacctcgcg gtcgaatgcc gccgcgacct cctgaacaag aagatcatgg tcgtcatcgg
     1141 cgagcgccaa tcgaacggcg tcgtcgaggc gcgccaggag aactacggcc cgcactacca
     1201 ggcgatcaac cgccgcctgc aggcactcgg cttgcggacg tacacgcccg agcagatcaa
     1261 gcgccagatc gcgcaggccg agctcgacgc gtacttccgc aacgatccgg acgccgcgct
     1321 cgccgcgtcg cgcaagctcg gcgcgagctt cgtcctgcgc gggctgatct ccgcggagac
     1381 gacgcgcaat ccgatgatgg ccgtgaacca ggtttcgctg aacatgggat tcacgctgac
     1441 cgcgagcaac ggccgcgtga tctccgatac gcaggcgacg gcgagctcgt acgccggcgc
     1501 cgacgtgcgg cgcatggcgc agacgctcgt cgacgagcag gccgacgagg tcgtcgcgaa
     1561 gctctacggc gattattgcc gcaacgccgc gcgcacccga taacgagtaa ccgggcgcgc
     1621 tgcgcgtccg tacgaagcaa cgtcaaccgg agtgaacgtc atgtcgaagc gtcttgcccg
     1681 tctcgccccg ccgctcgccg gcgccgtcat cgcgctctgc agcgcccctc tcctcgcgca
     1741 gcccacgttc ggcaatccgt cgccgaccgc cggcagcgaa acgtcgcaac gcgcgaacga
     1801 ttcggccgac caggcgctgt accggccggt cgagtacgtg aacaaggcga ggaaggggcc
     1861 tgcggtgatc gtgatccccg gcgagatcaa gagcaacaat gcgacattca tgcagaagtt
     1921 cacggcgaac aacatcgccg acttcggcga gctcgagatg tcgaacgcca atttccccgt
     1981 gctcgagcgc tcgaacctcg gaccgctgct caacgagttc cagctcgcct acaacctcgg
     2041 agatccgcag gccgcgcgca agttcctcgg catgggcaag ctcaagagca ccaagtacgt
     2101 cgtcaagttc gacatcctga aggccgagga agtcgcggcc gcgcagcagg gcttcgacgg
     2161 ccgcgcgctc ggcaacatcg ccggcgcgct cggcgcgttc agcggctcgt ggggtggcgc
     2221 acgcgccggc gccgtcggct ccgcggcggt cggctcggtg cacaccggcg aatcgaccgg
     2281 cgtgtggatc atcggcatgc gttacaagat catcaacgcc gagaccaccg agcaggtcgc
     2341 gcagggctac accgaggaga agatggaagt cggcgcgaag tcgagctcgg tgctcggcgt
     2401 gtcgcagtcg caacagggcg gcgtctcgct cgacacgatg gtgcaacgac tcgtgcagaa
     2461 gtccgtctgg gacatcgaca gcaagtacaa gtaggcatga gcgcgccggg ccgtcccaag
     2521 cgcgaattgc gcccactggg gggcagcgca gcggcgaagc cgcaagcgtg gggggaacgt
     2581 acaagcgcgg cgcgcgaggc gaagccgagc ggaggaggac tgcgcgcgag gcgcgaagcg
     2641 caggtccgaa ggggccgccc gagcgcgcgg tccgaagccc gacacgcgat gtggcgaatc
     2701 gatctgccag cacaaggagg gcgcccgaag ggcccgacga cgcgcgagtg ccgggaggcc
     2761 gacgctcgga cccgcgcata ccgccggact gccaaggagg ccgacgcgtg agcgccgtgt
     2821 catgatcgaa cggctcggca agta
//
LOCUS       DASSNN010000689         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_621835, whole genome shotgun sequence.
ACCESSION   DASSNN010000689 DASSNN010000000
VERSION     DASSNN010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621835"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFL84_12935"
     CDS             <1..257
                     /locus_tag="VFL84_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609579.1"
                     /translation="LNFGVGYDRIDRDFTDTVGAQPDGRDDWTGAWVSRAVGRRFSLG
                     VAISSYDRSGIESYDEQRYELRIAYSPTDSAAASMGSVGR"
     gene            357..1415
                     /gene="rfbB"
                     /locus_tag="VFL84_12940"
     CDS             357..1415
                     /gene="rfbB"
                     /locus_tag="VFL84_12940"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011771700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET7609580.1"
                     /translation="MNILITGGAGFIGSATIRYLIENTTARVVNVDSLTYAGNLESLG
                     RAAASERYAFEKADICDKKRLTQIFRTHQPDAVMHLAAESHVDRSIDGPEAFFQTNVT
                     GTGRLLQTATEYWNSSKDEKRAAFRFLHISTDEVFGDLKAGDEPFCETTAYAPSSPYA
                     ATKAASDHLVRAWGRTYKLPILITNCSNNYGPYQFPEKLIPHMILNALSGKPLPVYGD
                     GRQIRDWLYVEDHARALWTVLTRGRVGETYNIGGRNEQRNIDVVRTICSLLQSSVPAA
                     RRYEELITYVTDRPGHDSRYAINAGKIERELGWTPAETFETGLAKTVTWYLENRAWWQ
                     RVLDGSYRLVRIGTSAAS"
     gene            1412..2287
                     /gene="rfbA"
                     /locus_tag="VFL84_12945"
     CDS             1412..2287
                     /gene="rfbA"
                     /locus_tag="VFL84_12945"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET7609581.1"
                     /translation="MRRGIILAGGSGTRLHPVTIGVCKQLLPIYDKPMIYYPLSTLML
                     AGIRDVLVISTPQDTARFEQILGDGDQWGMNIQYAVQPKPEGLAQAFIIGERFVRNEP
                     STLILGDNLFFGSGLTGLLQEASQISDGATVFAYRVSSPEAFGVVAFDASGRATSIEE
                     KPARPKSNYAVTGLYFYDSNAVAIAKSLKPSARGELEISDLNSRYLEQGRLNVQRMAR
                     GTAWLDTGTHDSLLEAAHFIQTLEKRQGLKVGCPEEVAWRLGWIDAGKLEQLATRLKK
                     SGYGDYLLGLLEQGS"
     gene            2284..>2624
                     /gene="rfbC"
                     /locus_tag="VFL84_12950"
     CDS             2284..>2624
                     /gene="rfbC"
                     /locus_tag="VFL84_12950"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET7609582.1"
                     /translation="MKVTKTDLPGVLIIEPKAFGDERGFFLETFQSERYREAAGISLP
                     FVQDNHSRSKRGVLRGLHLQTRHVQGKLVRAARGEIFDVAADIDPKSPTFGRWVGVTL
                     SDTNHRQLWIPP"
BASE COUNT          487 a          875 c          842 g          420 t
ORIGIN      
        1 ggctcaactt cggcgttggc tatgacagga tcgaccgcga cttcacggac accgtcggcg
       61 cgcagcccga cgggcgcgac gattggaccg gcgcgtgggt gagccgcgcc gtgggccggc
      121 gattcagctt gggcgtcgca atttcgagct acgaccggtc cggcatcgag tcgtacgacg
      181 agcagcgtta cgagctcaga attgcctaca gcccgaccga tagcgccgcc gcgtcgatgg
      241 ggtcggttgg ccggtaacgg cgcgggggct gccggttttc ggggatttgt tcaaggtttt
      301 gcggccgaaa gtcacataaa atcgcggcca gcatcatcca ggcgccagga ccgaacgtga
      361 acatcctcat caccggcgga gcaggcttca tcggctccgc gacgatccgt tacctgatcg
      421 agaacacgac ggctcgcgtc gtcaacgtgg acagcctcac ctacgccggc aatctcgagt
      481 cgctcggccg cgcggccgcg tccgagcgtt acgcgttcga gaaagccgac atctgcgaca
      541 agaagcgctt gacgcagatc ttccgcacgc accagcccga cgccgtcatg cacctcgcgg
      601 ccgagagcca cgtcgaccgc tcgatcgacg gccccgaggc gttcttccag acgaacgtca
      661 cgggcacggg ccggctgcta cagacggcga cggaatactg gaattcgtcg aaggacgaga
      721 agcgcgcggc gtttcgtttc ctgcacatct cgaccgacga ggtcttcggc gatctcaagg
      781 ccggcgacga gcctttttgc gagacgacgg cgtacgcgcc gagctcgccg tacgcggcca
      841 cgaaggccgc gtccgaccat ctcgtgcgcg cctggggccg cacgtacaag ctgccgatac
      901 tgatcacgaa ctgctcgaac aactacgggc cgtaccaatt ccccgagaag ctgattccgc
      961 acatgatctt gaacgcgctg tcgggtaagc cgctgcccgt ctacggcgac ggccggcaga
     1021 tccgcgactg gctctacgtc gaggaccacg cgcgcgcgct ctggaccgtg ctcacgcgcg
     1081 ggcgcgtcgg cgagacctac aacatcggcg gccgcaacga gcaacgcaac atcgacgtcg
     1141 tgcgcacgat ctgctcgctg ctgcaaagca gcgtgcccgc ggccaggcgc tacgaagagc
     1201 tgatcacgta cgtgaccgat cggccgggcc acgattcgcg ctatgcgatc aacgcgggca
     1261 agatcgagcg cgagctcggc tggacgccgg ccgagacgtt cgagacgggc ttggcgaaaa
     1321 ccgtcacgtg gtatctcgag aatcgagcgt ggtggcagcg cgtgctcgac ggcagctatc
     1381 ggctcgtcag aatcggcacg agcgccgcct catgagacgc ggcatcatcc tcgccggcgg
     1441 ctccggcacg cgcttgcatc ccgtcacgat cggcgtctgc aagcagctgc tgccgatcta
     1501 cgacaagccg atgatttatt acccgctgtc gacgctgatg ctcgccggca tccgcgacgt
     1561 gctcgtgatc tcgacgccgc aggacacggc ccggttcgag caaatacttg gcgacggcga
     1621 tcagtggggc atgaacattc agtacgccgt gcagccgaag ccggaaggcc tcgcgcaagc
     1681 gttcatcatc ggcgagcgct tcgtgcgcaa cgagccgagc acgctgatcc tcggcgacaa
     1741 cttgttcttc ggcagcggct tgaccggcct gctacaggag gccagccaga tctcggacgg
     1801 cgcgacggtg ttcgcttacc gcgtctcctc gcccgaagct ttcggcgtcg tggccttcga
     1861 cgcgagcggg cgtgccacgt cgatcgagga aaaacccgcg aggccgaaga gcaattacgc
     1921 ggtcacaggt ctctacttct acgactcgaa cgccgtcgcg atcgcgaaat ccctgaagcc
     1981 ttccgctcga ggcgagctcg agatctccga cctcaacagc cgttacctcg agcaaggccg
     2041 gttgaacgtg cagcgaatgg cccgaggcac ggcgtggctc gacacgggca cgcacgactc
     2101 gctgctcgag gcggcccact tcatccagac gctcgagaag cgccagggtc tcaaggtggg
     2161 ctgccccgaa gaggtcgcat ggcggctcgg ctggatcgac gccggcaagc tcgagcaact
     2221 cgccacccgg ctcaagaaga gcggctacgg cgactacttg ctcggcctgc tggagcaggg
     2281 ctcgtgaaag tcacgaagac ggacctgccc ggagtcctga tcatcgagcc gaaagcgttc
     2341 ggcgacgagc gcggcttctt tctcgagacg ttccagtccg agcgctaccg cgaggcggcc
     2401 ggcatctcgc tgccgttcgt gcaggacaac cactcgcgct cgaagcgcgg cgtgctgcgc
     2461 ggcctgcacc ttcagacacg ccacgtgcaa ggcaagctcg tgcgcgccgc gcgcggcgag
     2521 atattcgacg tcgccgcgga catcgatccg aagtctccga cgttcggccg ctgggtcggc
     2581 gtgacgttgt cggacacgaa tcatcgccag ctctggattc cgcc
//
LOCUS       DASSNN010000690         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_980570, whole genome shotgun sequence.
ACCESSION   DASSNN010000690 DASSNN010000000
VERSION     DASSNN010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980570"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFL84_12955"
     CDS             <1..441
                     /locus_tag="VFL84_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7609583.1"
                     /translation="ENVGFVFQHFHLLDDLTVYENLEIPLSYRDVARKEREARVAEIL
                     DRFQMVGKKDLYPTQLSGGQQQQVAVARAVIANPRVVLADEPTGSLHWSQGEEIMKLL
                     KGLNDNGTTIVQVSHDERVAAFGRRLIELGDGWISSDRKIASTR"
     gene            442..1800
                     /locus_tag="VFL84_12960"
     CDS             442..1800
                     /locus_tag="VFL84_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7609584.1"
                     /translation="MAPRQPRIFVADDQRDVVESLRLLLKSEGYAAETFGNTESLVGA
                     LRERTADAVLMDLNYTRDTTSGDEGLDAVTRIRSFDAHTPIVLMTAWGTINLAVEGMR
                     RGAQDFIEKPWDNERLLAVLRTQVALGRALQRARTLEAENRQLVGGGDSLIAESPAMQ
                     PVLETIERVAPTDANVLITGESGCGKGLVARLIHEGSPRRERSLITVNIGSLAETVFE
                     SEMFGHVKGAFTDAKNDRVGRFELADGGTLFLDEIANIPVAQQGRLLRVLEDGQFERL
                     GSSRTQRADVRVLAATNADIDAEITAGRFRADLLYRLNTIHIHLPPLRERGDDILRLA
                     QRFLTQHAQHHRRGVTQFSEPALSALRRYSWPGNVRELNHVIERAVLMARGSAIEPAD
                     LRLIAAPPSSADRRPMTLDEVELDAIRAALARHDGNVVAAAEELGMSRSALYRRMEKF
                     GL"
     gene            1797..>2323
                     /locus_tag="VFL84_12965"
     CDS             1797..>2323
                     /locus_tag="VFL84_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HET7609585.1"
                     /translation="MTRRKRLRFQNRVLLITLLTVAPFAVATTVMLFLSDVAAGLRWT
                     ILGFLAFGVLIGLVAIRRHVVRPLQTLANMLEALREGDYSMRGRNVDPEDSFGEVMVE
                     VNTLSRTLYDQRLEALEAGALLQKIIADVDIAVFAFDAALRLRIVNRAGQVLLGGPSE
                     ALQGRSAAELGLSGML"
BASE COUNT          376 a          782 c          792 g          373 t
ORIGIN      
        1 gagaacgtcg gcttcgtgtt ccagcatttt catttgctcg acgacctgac ggtctacgag
       61 aacctcgaga tcccgctctc gtatcgcgac gtcgcgcgca aggagcgcga ggcccgcgtc
      121 gcggagatcc tcgatcggtt ccagatggtc ggcaagaagg atctctatcc gacgcagctc
      181 tcgggcggac agcagcagca agtcgccgtc gcgcgcgccg tgatcgcgaa cccgcgtgtc
      241 gtgctcgccg acgagccgac cggcagcctg cactggagcc agggcgaaga gatcatgaag
      301 ctcttgaaag gcttgaacga caacggcacg acgatcgtgc aggtctcgca cgacgagcgc
      361 gtcgccgcgt tcggccggcg gttgatcgag ctcggcgacg gctggatcag ctcggaccgc
      421 aagatcgcga gcacgcgata gatggcgccg cgccaaccgc gcattttcgt tgcagatgat
      481 cagcgcgacg tcgtcgagtc gctgcgcttg ctcttgaaga gcgaaggcta tgccgccgag
      541 acgttcggca acaccgagtc gctcgtcggc gcgctgcgcg agcgcacggc cgacgccgtg
      601 ctcatggatt tgaactacac gcgcgacacg acgtccggcg acgaaggcct cgacgctgtc
      661 actagaatcc gctcgttcga cgcgcacacg ccgatcgtgc tgatgacggc gtggggcacg
      721 atcaacctcg ccgtcgaagg catgcgccgc ggcgcgcagg acttcatcga gaagccctgg
      781 gacaacgagc gcctgctcgc cgtgctgcgc acgcaagtcg cgctcggccg cgcgctccaa
      841 cgcgcgcgca cgctcgaggc cgagaaccgc cagctcgtcg gcggcggcga ttcgctgatc
      901 gcggaatccc cggcgatgca gcccgtgctc gagacgatcg agcgcgtcgc gccgaccgat
      961 gcaaacgtgc tgatcacggg cgagagcggc tgcggcaagg ggctcgtcgc gcggctgatc
     1021 cacgaaggct cgccgcggcg cgagcgcagc ttgatcactg tcaacatcgg cagcttggcc
     1081 gagacggtgt tcgagagcga gatgttcggc cacgtcaaag gcgcgttcac ggacgcgaag
     1141 aacgaccgcg tcggccgctt cgagctcgcc gacggcggca cgctgttctt ggacgagatc
     1201 gcgaacatcc ccgtcgcgca gcaagggcgc ctcttacgcg tgctcgagga cggccagttc
     1261 gagcggctcg gctcgtcgcg aacgcaacgc gccgacgtgc gcgtgctcgc ggccacgaac
     1321 gctgacatcg acgccgagat cacggcgggg cggttccgcg ccgacctgct gtatcgcttg
     1381 aacacgatcc acattcacct gccgccgttg cgcgagcgcg gcgacgacat ccttaggctg
     1441 gcacagcggt tcctgacgca gcacgcgcag caccatcgcc gcggtgtcac gcagttcagc
     1501 gagccggcgt tgtcggcgct gcgccgctat tcctggcccg gcaacgtccg cgagctgaac
     1561 cacgtcatcg agcgcgccgt gctcatggcg cgcggctcgg ccatcgagcc cgcggatctg
     1621 cggctcatcg cggcgccccc gtcgtcggcc gatcgccggc cgatgacgct cgacgaggtc
     1681 gagctcgatg cgattcgcgc cgcgctggcg cgccacgatg gtaatgtggt cgcggccgcc
     1741 gaggagctcg ggatgagccg ctcggcgctc tatcggcgca tggagaagtt cggcttgtga
     1801 cgcggcgcaa gcgcctgcgt tttcagaacc gggtgctgct cattacgttg ctgacggtcg
     1861 caccgttcgc ggtggcgact acggtgatgc tgttcctgtc ggatgtggct gccgggctgc
     1921 gctggacgat tctcggcttc ctcgcgttcg gcgtgctgat cggcctggtt gccatccgcc
     1981 gtcacgtcgt gcggccgctc cagacgctcg cgaacatgct cgaggcgctg cgcgaaggcg
     2041 actactcgat gcgcggccgc aacgtcgatc ccgaagactc gttcggcgag gtcatggtcg
     2101 aggtcaacac gctgtcgcgc acgctctacg accaacgcct cgaagcgctc gaggccggcg
     2161 cgctgctgca gaagatcatc gccgacgtcg acatcgccgt gttcgcgttc gatgcggcgc
     2221 tgcgcttgcg catcgtcaac cgagcgggcc aggtgctgct cggcggcccg agcgaggcgc
     2281 tgcaaggccg cagcgccgcg gagctcggat tgagcggcat gct
//
LOCUS       DASSNN010000691         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_352444, whole genome shotgun sequence.
ACCESSION   DASSNN010000691 DASSNN010000000
VERSION     DASSNN010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352444"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /locus_tag="VFL84_12970"
     CDS             <1..1941
                     /locus_tag="VFL84_12970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HET7609586.1"
                     /translation="RTLRYDDFSAYYRRVRRRFESALAAPNGTEPYPVEHCTLCEFRG
                     VCHDRWRGEDSLVLVAGVRRTQVAGLRDARLPTLTALAMAAPRTRVAHVEAHAFEKLR
                     DQAALQLERRMSGRLDWHRIDAEAGHGFEQLPRPSPGDVVFDIEGDPFWEPAQGLHFL
                     FGILVRDGDGWQYRPMWAHDRVGERRMFEDFVDLVHARLAADPAMHVYHYGTYENAAL
                     KQLMGAYATREDAVDELLRRGVLVNLHTVVRQGLRAGVESYSLKEVEGLVPYARRADV
                     RSGMGAVLAYEEWMTARDDALLASIADYNEDDCRATLALRDWLVAQHPNDLSWLGPRA
                     SVEDKDAEEAGARAELRRQLVEGEEPGSPRWLAGELLEYHQREARPVWWWFFERSDHM
                     TTDELLDDGESIAGLAPAGAPTADRKSRVYPLTFPVQQHKFRPGDSPYDPATKKGAGT
                     IVALDDIAGTLALKRGPKLSDVPLPRALIPGGPYKTDAQRDALARFARSMLADDARYP
                     AVRDILARTPPRVRGRESGGDIQTIDIVEQRALTLALESSYLFIQGPPGTGKTWTGAR
                     LITELMRNGRRVGVTATSHKAIHNLLDEVERAAKEEGLRFRGLKKCSSDNAESLYDSA
                     SIASASASDTCPASSMISVSTA"
     gene            complement(1860..>2069)
                     /locus_tag="VFL84_12975"
     CDS             complement(1860..>2069)
                     /locus_tag="VFL84_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011714303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609587.1"
                     /translation="NAESLYDSASIASAGEIKDFANAGPDTLLFAGTAWLFAHRDVDQ
                     AVDTLIIDEAGQVSLADALAIDALS"
BASE COUNT          341 a          680 c          736 g          312 t
ORIGIN      
        1 cggacgctgc gatacgacga cttctcggcg tattaccggc gcgtgcgccg ccggttcgaa
       61 tcggcactcg ccgcgcccaa cggtaccgag ccgtatcccg tcgagcactg cacgctgtgc
      121 gaattccgtg gcgtctgcca cgaccggtgg cgtggcgaag acagcctggt gctcgtcgcc
      181 ggcgtgcgcc gcacgcaggt cgccggcctg cgcgacgcgc gcctgccgac gctcacggcg
      241 ttggcgatgg cggccccgcg aaccagggtc gcgcacgtcg aagcacacgc gttcgagaag
      301 ctgcgcgacc aggcggcctt gcaactcgag cgacgcatgt cgggccggct cgactggcat
      361 cggatcgacg ccgaagcggg ccacggcttc gagcaactgc cgcggccatc gcccggggac
      421 gtggttttcg acatcgaagg cgatccgttc tgggaacccg cgcaggggct gcacttcctg
      481 ttcggcatcc tcgttcgcga cggcgacggt tggcaatacc ggccgatgtg ggcgcacgat
      541 cgtgtcggcg agcggcggat gttcgaggat ttcgtcgacc tcgttcatgc gcgccttgcc
      601 gccgatccgg cgatgcatgt ataccactac ggcacctacg agaacgcggc gctcaagcag
      661 ttgatgggcg cgtacgcgac gcgcgaggat gccgtcgacg agctgttgcg ccgcggcgtg
      721 ctcgtcaatc tgcatacggt cgtgcggcag ggcttgcgcg cgggcgtcga aagctactcg
      781 ctgaaagagg tggaggggct cgtgccgtac gcacggcggg ccgacgtgcg cagcggcatg
      841 ggcgcggtgc tcgcgtacga ggaatggatg accgcgcgcg acgacgcgtt gcttgcatcg
      901 atcgccgact acaacgaaga cgattgccgc gcgacgctcg cgttgcgtga ctggctggtc
      961 gcgcagcatc caaatgatct ttcgtggctc ggcccgcgcg cgagcgtcga ggacaaggat
     1021 gcggaggaag ccggcgcgcg cgccgagctg cggcggcagc tcgtggaggg cgaggagccc
     1081 ggatcgccgc ggtggctggc gggcgagctg ctcgaatatc accagcgcga ggcgcggccg
     1141 gtgtggtggt ggttcttcga gcgcagcgat cacatgacca cagacgagct gctcgacgac
     1201 ggcgagtcga ttgcgggcct cgcgccggcc ggggcgccga ctgcggacag gaagtcgcgc
     1261 gtttacccgc tgacgttccc ggttcagcag cacaagttcc gaccgggaga ttcgccgtac
     1321 gacccggcaa cgaagaaggg cgccggcacg atcgtcgcgc tcgacgacat cgcgggcacg
     1381 ctggcgctca agcgcggacc gaagctcagc gacgtgccac tgccgcgcgc gctgattccc
     1441 ggcggcccgt acaagaccga cgctcagcgc gacgcgctcg cgcgcttcgc tcgatcgatg
     1501 ctcgcggacg acgcgcgtta tcctgcggtg cgcgacatcc ttgcgcggac gccgccgcgc
     1561 gtgcgcgggc gcgaaagcgg cggcgacatc cagacgatcg acatcgtcga gcagcgcgcg
     1621 ctgacgctgg cgctcgagtc gagctacctg ttcattcagg gaccgccggg caccggcaag
     1681 acgtggaccg gcgcgcggct catcaccgag ctcatgcgaa atggccggcg cgtcggcgtc
     1741 acggcaacta gccacaaggc aatccacaac ctgctcgacg aggtggagcg ggccgcgaaa
     1801 gaggagggcc tgcgctttcg cggcttgaag aagtgctcga gcgacaacgc cgagtcgctc
     1861 tatgacagcg cgtcgatcgc aagcgcgtcg gccagcgaca cctgccctgc ttcatcgatg
     1921 atcagcgtgt cgaccgcctg atcgacgtcc cgatgggcga agagccaggc agtgccggca
     1981 aacagcaacg tgtcaggacc cgcgttggcg aagtccttga tttcgcccgc gcttgcgatc
     2041 gacgcgctgt catagagcga ctcggcgtt
//
LOCUS       DASSNN010000692         2668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_981206, whole genome shotgun sequence.
ACCESSION   DASSNN010000692 DASSNN010000000
VERSION     DASSNN010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981206"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFL84_12980"
     CDS             complement(<1..381)
                     /locus_tag="VFL84_12980"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HET7609588.1"
                     /translation="MLRVAGGVLWTAINRMFEDDALPLAAALAYYSLLSMAPLLLVAV
                     ALAGTFFADGQVHEQLIEEMRRLTGDAGATLTQTVIANTGSQGRSAWSLALGAFLTLL
                     GASTVFGQLQYALNRVWRVKAAPRA"
     gene            447..1634
                     /locus_tag="VFL84_12985"
     CDS             447..1634
                     /locus_tag="VFL84_12985"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HET7609589.1"
                     /translation="MRNGLRRAAIVIGVLVVIGIAARLAAPTLVERYVNRQLADMGEY
                     RGTVSRVELRLLAGGYVLRDLTIVETDSTNETPFVTMPAMDLTLQWRALSRGRAVGEV
                     VMHDPVVNFIQGPSDEQQQLGAGVNWPQEIRELFPFRLNVVEARNGLVTFRAPGIDTN
                     ESLTIRDFQARLRNLTNVQGVAEPAFAEVDVRGGIMGNAPLTLTGRIDPNERTPTFDI
                     DFSIEGAQLVDVNPWLREFLNVDAEMGAFSMYAELAAAEGKFEGYVRPILENPKFVDR
                     DDDEKGPFRKAWEGLVNVAAKILENRQEEQVATQIPLRGEIEDPDVDLLTTIVNLLRN
                     AFVAAFSHSLEGSINLRDVAGDVLGDDSAADASKAPQDGAQEPSRRERREERRRNREA
                     NQQ"
     gene            complement(1700..>2668)
                     /locus_tag="VFL84_12990"
     CDS             complement(1700..>2668)
                     /locus_tag="VFL84_12990"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HET7609590.1"
                     /translation="NGNDPGLVVGKAAADALLATEYRSDNGAAFNGEAVVGKWRPTPP
                     ALATMAFVYAATTRPFVMDKPEQFRPGPPPAVTSDKYTREYNEAKAKGSVLSHPATNA
                     CPAPADTDKARFWAGNFGAQWNQTIRDVAVDRQLGVGDTARLLALANLAVADAGIAVW
                     DAKIHYNYWRPITAIREGDSDGNPNTAGDAGWTPFIQTLSAQTPPYPDYTSGANGVTA
                     AYATILQLFFKSDFVPLAINKAPLAATVPICTTPRLFRRISDAMDEVVEARILLGIHF
                     RSADVVARQTGTRVAWNTFTHALRPLHNNYHGFGYGHGDDDDDSQH"
BASE COUNT          446 a          910 c          890 g          422 t
ORIGIN      
        1 cgcgcgaggc gcggccttga cgcgccagac gcggttcaac gcgtactgca gttggccgaa
       61 cacggtgctc gcgccgagca acgtcaggaa cgcgccgagc gcgagcgacc atgcgctgcg
      121 cccctggctg cccgtgttcg cgatgacggt ctgcgtcagc gtcgcgcccg catcgcccgt
      181 tagccggcgc atctcttcga tgagctgctc gtgcacctgg ccgtcggcga agaacgtgcc
      241 cgcgagcgcg acggccacga gcaacagcgg cgccatcgac agcagcgagt agtacgcgag
      301 cgcggccgcg agcggcagcg cgtcgtcctc gaacatccgg ttgatggcgg tccagagcac
      361 gccgccggcg actctcagca tatcggccag tcagcaagta ccgtgccacg gcggctacgt
      421 ggcacgagcc ttgcagccga cagcgcatgc gtaacggact gcgccgcgcg gccatcgtca
      481 tcggcgtcct cgtcgtcatc ggcatcgccg cacgcctcgc ggcgcccacg ctcgtcgagc
      541 gctacgtcaa ccggcagctc gccgacatgg gcgagtatcg cggcaccgtc agccgcgtcg
      601 agcttcgcct cctggccggc ggctacgtgc tgcgcgacct cacgatcgtc gagaccgatt
      661 cgacgaacga gacgccgttc gtgacgatgc cggccatgga tctcacgctg cagtggcgcg
      721 cgctgtcccg cgggcgcgcc gtcggcgagg tcgtcatgca cgacccggtc gtgaatttca
      781 tccaggggcc gtccgacgag cagcagcagc tcggcgccgg cgtcaactgg ccgcaggaga
      841 tccgcgagct gtttccgttc cggctcaacg tcgtcgaagc acgcaacggc ctcgtgacgt
      901 ttcgcgcgcc cggcatcgac acgaacgaat cgctgacgat ccgcgacttc caggcgcggc
      961 tgcgcaactt gacgaacgtg caaggcgtcg ccgagccggc gttcgcggag gtcgacgtgc
     1021 gcggcggcat catgggcaac gcgccgctga cgctgaccgg gcgcatcgac ccgaacgaga
     1081 ggacgccgac gttcgacatc gacttctcga tcgaaggcgc gcagctcgtc gacgtgaatc
     1141 cgtggctgcg cgaattcctg aacgtcgacg ccgagatggg cgcgttctcg atgtacgccg
     1201 agctcgccgc cgccgaaggc aaattcgaag gctacgtgcg gcccatcctc gagaatccga
     1261 agttcgtcga ccgcgacgac gacgagaaag gtccgtttcg caaagcgtgg gaaggcctcg
     1321 tcaacgtcgc ggcaaaaatt ctcgagaacc gccaggaaga gcaagtcgcg acgcagatcc
     1381 cgctgcgcgg cgagatcgag gaccccgacg tcgacctgct gacgacgatc gtcaatctgt
     1441 tacgcaacgc gttcgtcgcc gcgttctcgc actcgctcga aggctcgatc aatctgcgcg
     1501 acgtcgccgg tgacgttctc ggcgacgatt ccgctgccga cgcctccaaa gccccgcaag
     1561 acggcgcgca agaaccgtcg cgccgcgagc ggcgcgagga gcgccgccgc aaccgcgagg
     1621 cgaatcagca ataaataaaa ccggatcgag gagaggcggc tgcctctcga tcgagccggt
     1681 ctcgacgcac gtgactcagc tagtgctgcg agtcgtcgtc atcgtccccg tggccgtagc
     1741 cgaagccgtg gtaattgttg tgcagcggcc gcagcgcatg cgtgaacgta ttccacgcga
     1801 cgcgcgtccc cgtctgtcga gccaccacgt cggcactacg gaagtggatg ccgagcagga
     1861 tgcgcgcctc tacgacctcg tccatcgcgt ccgagatgcg gcggaacagc ctcggcgtcg
     1921 tgcagatcgg cacggtcgca gcaagcggcg ccttgttgat cgcgagcggc acgaagtcgc
     1981 tcttgaagaa gagctgcagg atcgtcgcgt acgcggccgt gactccgttc gcgcccgacg
     2041 tgtagtccgg gtacggcggg gtctgcgcgc tcaacgtctg gatgaacggc gtccaacccg
     2101 cgtcgccggc cgtgttcggg ttcccgtcgc tgtcgccttc gcggatcgcc gtgatgggcc
     2161 gccagtagtt gtagtggatc ttggcatccc agactgcgat gccggcgtcc gcgacggcga
     2221 ggttggcgag cgcgagcagg cgcgccgtgt cccccacccc aagctgtcga tcgaccgcga
     2281 cgtcgcggat ggtctgattc cactgcgcgc cgaagttgcc ggcccaaaac cgcgccttgt
     2341 cggtgtcggc cggcgccggg caagcattcg tagcgggatg cgacagcacc gagccctttg
     2401 ctttggcctc gttgtactcg cgcgtgtatt tgtcgctcgt cacggccggc ggcggacccg
     2461 ggcggaactg ctccggctta tccatcacga acgggcgtgt cgtcgcggcg tagacgaacg
     2521 ccatcgtcgc aagggccgga ggcgtcgggc gccatttgcc gacaacggct tctccgttga
     2581 acgcggcacc gttgtccgaa cggtactcgg tcgcgagcag cgcatcggcg gccgccttgc
     2641 cgacgacgag acccgggtcg ttaccgtt
//
LOCUS       DASSNN010000693         2550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_494438, whole genome shotgun sequence.
ACCESSION   DASSNN010000693 DASSNN010000000
VERSION     DASSNN010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494438"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..657
                     /locus_tag="VFL84_12995"
     CDS             409..657
                     /locus_tag="VFL84_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609591.1"
                     /translation="MPPVAQAVLQELRMRYQAAYTAYQACVRAVTEATMSGSTPSSML
                     LEQEAKALGALTKTRASLLAAMAEDAGAAESMPIGPPG"
     gene            complement(713..788)
                     /locus_tag="VFL84_13000"
     tRNA            complement(713..788)
                     /locus_tag="VFL84_13000"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(752..754),aa:Glu,seq:ctc)
     gene            860..2191
                     /gene="pcnB"
                     /locus_tag="VFL84_13005"
     CDS             860..2191
                     /gene="pcnB"
                     /locus_tag="VFL84_13005"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HET7609592.1"
                     /translation="MNDTELESRHGPVIVPRSEHNISRANISPNALKVLYRLKSAGFE
                     AFLVGGSVRDLLLDRHPKDFDVVTNADPDQIQDLFRNCRLIGRRFRLAHVRFGRDIVE
                     VATFRAAGAEPNDDDRAHSDSGRILRDNVYGTIGEDVWRRDFTVNALYYNITDFSIWD
                     YVDGVGDVRARTLRLIGDPEKRYREDPVRMLRAVRFAAKLDFTLAPETEAPLASLGKL
                     LADVPPARLFDELLKLFQSGHAVQSFDLLLRYDLLRYLFRDTQAALDTPDRDKIIDFI
                     RRGLANTDQRVREDKPVTPMFLYALFLWFPIQALARRLESEGWNDGQAMLEASQRIVA
                     TQQTAFPRRFSSPMKELLTMHWRFGQRHGARAARLLQHKRFRAAYDFLVLRASCGEVE
                     QEVADWWTELTSLPPEEQSKRLNVGRRQGGRRRRGGRRRAGRHHEINDVVV"
     gene            2286..>2550
                     /locus_tag="VFL84_13010"
     CDS             2286..>2550
                     /locus_tag="VFL84_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HET7609593.1"
                     /translation="MPLDGPRYIVVEGPIGVGKTSLAKRLAKSFASELILERAEENPF
                     LEGFYRNRRQSALPTQLFFLFQRARQIEQIRQSDMFSPVRIADF"
BASE COUNT          472 a          829 c          843 g          406 t
ORIGIN      
        1 gtcatcgagt acgaaggcca tagccgcaac aacgtctacc gccgaggctg gcgccccgac
       61 atggtgcaca ccggtgacac gatcagcatc gacatcgcac ccatgcgaac cggagaggac
      121 ggcggctacg tcaagacgtg ggtcctcaaa gacggaaccg agttctagag cgaatcgatt
      181 ctcggcgaag ccgctgcagc ggcccggcat cagcccggcg caaaaaaaga gagcccgacc
      241 gcgaggccga gctctacagg gggcttcaag atctccaatc ggggaggaaa ttctcaaagc
      301 gggagtattg taagccgagc cgctggtcat cgcggtcaag aactgtcaag cgtctcttta
      361 ttgagacacc tggccctcgg ccgtgccggt gctcagaatg ccggacccat gccccccgtc
      421 gcacaagccg tgctgcaaga gctcaggatg aggtatcaag ccgcatacac ggcctatcag
      481 gcctgcgtcc gcgcggtgac cgaggcgacg atgagcggct cgacaccatc ctcgatgctg
      541 ctcgagcaag aggccaaagc gctcggtgcg ttgaccaaga cgcgagcgag ccttcttgcc
      601 gccatggccg aggatgccgg cgccgcggaa tcgatgccga tcggtccgcc gggctgacgc
      661 gcctcgatgc cgcgaatgtc gagcggcgtt gcgccgctcg cgccaatgga aatggcgctc
      721 cctgggggaa tcgaaccccc gttccagcct tgagagggcc gtgtcctggg ccgctagacg
      781 aagggagctt ggatgcccgc ctgaagcgag ggctggtatg ataccggcct ttctcgcagg
      841 gcgaaaccac ggcagcttgt tgaacgacac ggaactagag tcgcgccacg gtcccgtcat
      901 cgtcccgcgc tcggaacaca acatcagccg ggccaacatc tcgcccaacg ctctcaaagt
      961 cctgtaccga ttgaagagcg ccgggttcga ggcgtttctc gtgggcggca gcgtccgcga
     1021 cttgctcctc gaccggcatc cgaaggactt cgacgtcgtc acgaacgccg atcccgacca
     1081 gattcaggac ctctttcgca actgccggtt gatcggcagg cggtttcgtt tggcgcacgt
     1141 gcgcttcggc cgcgacatcg tcgaggtcgc gacgtttcgc gcggcgggcg ccgaaccgaa
     1201 cgacgacgat cgtgcccact ccgacagcgg ccgcattctg cgcgacaacg tctacggcac
     1261 gatcggcgag gacgtttggc gccgcgactt cacggtcaac gcgctctact acaacatcac
     1321 ggacttcagc atctgggact acgtcgatgg cgtcggcgac gtgcgcgcgc gaacgctgcg
     1381 gctgatcggc gacccggaga agcgctatcg cgaggacccg gtgcgcatgc tgcgcgccgt
     1441 gcggttcgcc gcgaagctcg acttcacgct cgcgcccgag acggaagcgc cgctcgcgtc
     1501 gctcggcaag ctgctcgcgg acgttccgcc tgcgcgcttg ttcgacgagc tcttgaagct
     1561 gttccagagc gggcatgccg tgcagagctt cgaccttctg ctccgctacg acttgctgcg
     1621 ttatctcttt cgcgatacgc aggccgcgct cgacacgccc gaccgcgaca agatcatcga
     1681 cttcatcagg cgcggcctcg ccaatacgga ccaacgcgtg cgcgaggaca agccggtcac
     1741 gccgatgttc ttgtatgcgc tgttcctgtg gttcccgatc caagcgctcg cgcgccgtct
     1801 cgagagcgag ggctggaatg acggccaggc gatgctcgag gcgagccagc gcatcgtagc
     1861 gacgcagcaa acggcgtttc cgcgccggtt cagctcgccg atgaaagagc tgctgacgat
     1921 gcactggcgt ttcgggcagc ggcacggcgc gcgtgcggcg cggctgcttc aacacaagcg
     1981 ctttcgagcg gcctacgact tcctcgtgct gcgggcgagc tgcggcgaag tagagcagga
     2041 agtcgcggac tggtggacgg agctcacgag cttgccgccc gaagagcaga gcaagaggct
     2101 caacgtgggc cgccgccagg gagggcggcg ccggcggggc gggcgccggc gcgccggccg
     2161 ccaccacgag atcaacgacg ttgtcgttta gcttcggggc gagagacaag cggccgaagg
     2221 ccgcgtcggc tcccgtcggc cgagcggccg cgaaacgcga cgcgggggac cagggagtgg
     2281 gtgccgtgcc gctcgacggg ccgcgctaca tcgtcgtcga gggcccgatc ggcgtcggca
     2341 agacgagcct cgcgaagcgg ctggcgaaga gcttcgcgag cgagttgatc ctcgagcgag
     2401 ccgaggaaaa cccgtttctc gaggggttct atcgcaaccg gcggcagtcg gcgttgccaa
     2461 cgcagctatt ctttctgttt caacgcgcgc ggcagatcga gcagatacgg caaagcgaca
     2521 tgttctcgcc cgtgcgcata gccgacttcc
//
LOCUS       DASSNN010000694         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_352631, whole genome shotgun sequence.
ACCESSION   DASSNN010000694 DASSNN010000000
VERSION     DASSNN010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352631"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VFL84_13015"
     CDS             <1..923
                     /locus_tag="VFL84_13015"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7609594.1"
                     /translation="RADRLAVVIGPCSIHDPAAALEYAARLKPLRETLGDALEIVMRV
                     YFEKPRTTVGWKGLINDPDLDGSFNINKGLRLGRRLLCDINELGLPAGVEYLDTISPQ
                     YISDLVAWGAIGARTTESQVHRELASGLSCPVGFKNGTDGNVKIAVDAVGAASHPHHF
                     LAVTKEGQATVAATAGNPDGHVILRGGKTPNYGAEHVAAACQLLAQADLPARLMIDAS
                     HANSLKRHENQPRVVEEIAAQIEGGEERIVGVMVESHLVGGRQDLTSGKPLVYGQSIT
                     DACIDWPTSVTVLDRLAAAVRARRRRAAAA"
     gene            1005..1406
                     /locus_tag="VFL84_13020"
     CDS             1005..1406
                     /locus_tag="VFL84_13020"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609595.1"
                     /translation="MRMRWPLFAIAAVLTGLAGVATAHHSFTATYFEDRTTEIQGKLL
                     QFMFRNPHSFVHVEAPDENGTMQRWAVEWGGAGQLSGQGVTNQTLRVGDIVSITGNPG
                     RDPADHRIRMQYLRRPADGFEWGRNPGETFN"
     gene            1418..>2167
                     /locus_tag="VFL84_13025"
     CDS             1418..>2167
                     /locus_tag="VFL84_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609596.1"
                     /translation="MRALLISASLCAIVVVAPARAQLAIVGEWTGRYHEDQGDRVPGD
                     VQGDFTGVPLNDAARKYAEAFDVTRVAVLEHQCQPYNVAHIYRGPNQFRIWEEKDPAT
                     QEILAYRIYNGTYQQYRTIWMDGRAHPPAYAPHTHMGFSTGQWHGDILTVNTTHIKKE
                     FYRRSGIPSSDMTTLVEHWIRHGNVLSHVSMITDPVYLTEPYVTSEEFVLMERGNQNW
                     LYNCEYVMEVPMDKNDVPFYLPGKNPFIEDWA"
BASE COUNT          401 a          731 c          705 g          330 t
ORIGIN      
        1 gccgagccga tcgcctcgcc gtcgtgatcg ggccctgctc gatccatgat ccggccgcag
       61 ccctcgagta cgcggcgcgt ctcaagccgc tgcgcgagac gctcggagat gcgctcgaga
      121 tcgtcatgcg cgtctacttc gagaagccgc gtacgaccgt cggctggaaa gggctcatca
      181 acgaccccga cctcgacggc agcttcaaca tcaacaaggg cttgaggctc gggcggcgct
      241 tgctctgcga catcaacgag ctcgggctgc cggccggcgt cgagtatctc gacacgattt
      301 cgccgcagta catctcggac ctcgtcgcct ggggcgccat cggcgcgcgc acgaccgaga
      361 gccaagtcca tcgcgagctc gcgtcgggtc tctcgtgccc ggtcggcttc aagaacggta
      421 ccgacggcaa cgtcaagatt gctgtggatg cggtcggtgc ggcatcgcat ccgcatcact
      481 tcctggccgt gaccaaggaa ggtcaggcga ccgtcgcggc gacggcgggc aatcccgacg
      541 gccacgtgat cctgcgcggc ggcaagacgc cgaactacgg cgccgagcac gtcgcggcgg
      601 cgtgtcagct gctcgcgcaa gccgacttgc cggcgcggct catgatcgat gcgagccacg
      661 caaacagctt gaaacggcac gaaaatcagc cgcgcgtcgt cgaggagatc gcggcgcaga
      721 tcgagggcgg ggaagagcgc atcgtcggcg tgatggtcga gagccacctc gtcggcggcc
      781 ggcaagacct cacgtccggt aagccgctcg tgtacggcca gagcatcacg gacgcctgca
      841 tcgactggcc gacgtcggtg acggttctcg accgcctcgc ggcggccgtc cgcgcacggc
      901 gccgccgagc ggcggcggct taacagcgcg gcggttccac cggtagaatt ccgctcgacg
      961 gtgggccgtc ggacggccgt tatcaccgca atcgggagag ctttatgcgc atgcgctggc
     1021 ccttgttcgc aatcgcggcc gtcctgacag ggctcgcggg cgttgccacg gcccatcatt
     1081 cgttcacggc gacctatttc gaagaccgca cgaccgagat ccagggcaag ctgctgcagt
     1141 tcatgttccg caacccgcat tcgttcgtgc acgtcgaggc gcccgacgag aacggcacga
     1201 tgcagcgctg ggccgtcgaa tggggcggtg ccggccagct atcgggccag ggcgtcacga
     1261 atcagacgct gcgcgtcggg gacatcgtct cgatcacggg caatcccggc cgagacccgg
     1321 ccgaccaccg catccgcatg cagtacctgc gccgcccggc cgacggcttc gaatggggca
     1381 ggaaccccgg cgagaccttc aactaggcca tagaagggtg cgagctctcc tcatcagcgc
     1441 ctcgctgtgc gcgatcgtcg tggttgcgcc ggcccgcgcc cagctcgcga tcgtcggcga
     1501 gtggacaggc cgctatcacg aggaccaggg cgaccgcgtg cccggcgacg tgcagggcga
     1561 cttcacgggc gtgccgctca acgacgcggc acgcaagtac gccgaggcgt tcgacgtcac
     1621 gcgcgtcgcg gtgctcgagc accaatgcca gccgtataac gtcgcgcaca tctatcgcgg
     1681 cccgaaccag ttcaggatct gggaagagaa ggatcccgcg acgcaggaga tcctcgcgta
     1741 ccgcatctac aacggcacgt accagcaata ccgcacgatc tggatggacg gccgcgcgca
     1801 tccgcccgcg tacgctccgc acacgcacat gggcttctcg acggggcagt ggcacggcga
     1861 catcctcacg gtcaacacga cgcacatcaa gaaagagttc taccgccgca gcggcatccc
     1921 gagcagcgac atgacgacgc tcgtcgagca ctggatccgc cacggcaacg tgctgtcgca
     1981 cgtttcgatg atcaccgatc cggtgtatct gaccgagccg tacgtgacga gcgaagagtt
     2041 cgtgctcatg gaacgcggca atcagaactg gctctacaac tgcgagtacg tcatggaagt
     2101 cccgatggac aagaacgatg tgccgttcta cctgccgggc aagaatccgt tcatcgagga
     2161 ctgggcc
//
LOCUS       DASSNN010000695         3531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1867122, whole genome shotgun sequence.
ACCESSION   DASSNN010000695 DASSNN010000000
VERSION     DASSNN010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867122"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VFL84_13030"
     CDS             <1..815
                     /locus_tag="VFL84_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7609597.1"
                     /translation="LMSEAVAVLEGSPSRLELVRALVDRGAYDPGYKSYPFGDTLLTI
                     ARDRGFDEIAGLLENALARGRARKWPETGEIDYEQDADQVRFDHAVHDGKRRDVERLL
                     AARPDLARNELSSWAEGVLMMPANRRNRALLEVLLLHGATVPLMSKWARFYYFKHDDI
                     AVFLMERGMSPHSRTWHGVTLLHDMAQNGDLAKARLLIDHGAELDVIEDEYRSTPLGL
                     AARWGRRSLVELLLARGANPNASGAPWSTPLAWARKKGHRELERVLVAAGAE"
     gene            837..923
                     /locus_tag="VFL84_13035"
                     /pseudo
     CDS             837..923
                     /locus_tag="VFL84_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404383.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            936..1319
                     /locus_tag="VFL84_13040"
     CDS             936..1319
                     /locus_tag="VFL84_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7609598.1"
                     /translation="MKRTWTIIGVADVARSFAWYQALFGQPKSAPAHDYFGQIVDTDG
                     TVLLCLHEWGPHGHPTLTSPDVAEPGNGLLLFFRVDDFDEALARARGLVRRLEEEPGI
                     NPATGTREFALRDPDGYYVMVSALG"
     gene            1479..2183
                     /locus_tag="VFL84_13045"
     CDS             1479..2183
                     /locus_tag="VFL84_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7609599.1"
                     /translation="MSVWPREFLVILGESGSGKTTLLKTINRLQPPDSGDVLVEGRST
                     SEVVEHELRRRIGYVFQRVGLFPHMTVAENIAVTPRLLGWSEDEVRARVEELLALVNL
                     PVEMAPRLPAALSGGQRQRVGVARALAARPAIMLMDEPFGALDPVTRDSLGGEYRQLH
                     ERMGLVTVMVTHDVLEALLLADRIVVMRDGGIVASGTPREMMNERGDAEVRRLMDMPR
                     RQMTRVAAMLDDARRP"
     gene            2180..>3531
                     /locus_tag="VFL84_13050"
     CDS             2180..>3531
                     /locus_tag="VFL84_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET7609600.1"
                     /translation="MNDRFASAWALLPGYLSQHVALSAAAMALGLVVSLALVVAAVRS
                     ERVRWPVLAFASLIQTVPSLALLALFYPLLLAISGLIESVFGTGFSALGFLPSLLALA
                     LYSMLPVIRNGVAGILNIDAAVIEAARGVGMTDWQRLRRVELPLAAPMLMAGIRTAAV
                     WVIGAATLSTPVGQVSLGNYIFSGLQLQNWVAVLFGCVAAAALALAVDQLLGLIETGL
                     ARRSRSRVAAGVAALAVGIGAAALPFGGDSAAAYVVGGKNFSEQFILAELMSARIAAS
                     GGAAARRTGLGSAVVFAALASGDIDVYVDYSGTIWANVMRRTDVPRREPMLAEMRAWL
                     EAEHGIVLLGSLGFENAYGLAMRRDRAERLGIATIADLAAHAAELSMGGDLEFFSRPE
                     WQALESSYGLAFATRREFEPTFMYRAVTDGEVDVISAFTSDGRIAANDLLVLADPKGA
                     "
BASE COUNT          511 a         1197 c         1233 g          590 t
ORIGIN      
        1 agctcatgtc cgaggcggtc gccgtcctcg agggctcgcc gtcgcggctc gagctcgtgc
       61 gcgcgctcgt cgatcgaggc gcctacgatc cgggctacaa gagctatccg ttcggcgaca
      121 cgctgctcac gatcgcgcgc gaccgcggtt tcgacgagat cgcggggctc ctcgaaaacg
      181 cgctcgcgcg cgggcgcgcg cgcaagtggc cggaaacggg cgagatcgac tacgagcagg
      241 acgccgacca ggtgcgtttc gatcacgcgg tgcacgacgg gaagcgccgc gacgtcgagc
      301 ggctgctcgc cgcgcgtccc gatctcgcgc gcaacgagct ctcgtcctgg gccgaggggg
      361 tgctgatgat gccggccaat aggcgcaacc gcgccctgct cgaggtgttg ttgctgcacg
      421 gcgcgacggt gccgctcatg tcgaagtggg cgcggttcta ttacttcaag cacgacgaca
      481 tcgcggtctt cctcatggag cgcggcatga gcccgcactc gaggacctgg cacggcgtga
      541 cgctgctcca cgacatggcg cagaacggcg atctcgcgaa agcgcgcctc ttgatcgacc
      601 acggcgccga gctcgacgtg atcgaggacg aatatcgctc gacgccgctc ggcctcgccg
      661 cgcggtgggg gcgacgatcg ctcgtcgagc tcttgctcgc gcgcggcgcg aatccgaacg
      721 cctcaggcgc gccgtggagc acgccgcttg cgtgggcgcg caagaaaggt catcgggagc
      781 tcgagcgcgt gctggtcgca gccggcgcgg agtgaatcgc ccgacgagtt tgggcgacgg
      841 cctgctcgga tatcgatatg atgagcgcca acaccgacgg ccggttcgtg cacaaggatg
      901 gcactcccta ttcaaagtca taacggtgag cagccgtgaa gcgcacctgg accatcatcg
      961 gcgtcgccga cgtcgcccgc agcttcgcct ggtatcaagc gctgttcggg cagccaaagt
     1021 ccgcgcctgc gcacgactac ttcggacaaa tcgtcgacac ggatggcacc gtcttgctct
     1081 gtcttcacga gtggggaccg cacgggcatc cgaccctgac cagtcccgac gtcgcggagc
     1141 ccggcaacgg gctgctgctg ttcttccgag tcgacgattt cgacgaggcg ctggcgcgag
     1201 cgagagggct cgtccgccgg ctcgaagagg aacctgggat caatcccgcc accggcacgc
     1261 gcgaattcgc gcttcgcgat cccgatgggt actacgtcat ggtcagcgct cttggctgag
     1321 catgtacgga gctcccgacg ccgcagagcg cgcatgctcg gccggcggcc ggcacgcgag
     1381 ccgtgactga cgccttggag ggtcgcggcc gaccgatcgt cgagctgcgt tcgattcggc
     1441 gcgccttcgg ctcgaccgta gcggttcgag atgtcgactt gagcgtctgg ccgcgcgagt
     1501 ttctcgtcat cctcggcgag tcggggtcgg gcaagacgac gctgctcaag acgatcaacc
     1561 gcttgcagcc cccggattcg ggcgacgtgc tcgtcgaagg gcgttcgacg agcgaggtcg
     1621 tcgagcacga gctgcggcgg cgcatcggct acgtgtttca acgcgtcggg ctctttccgc
     1681 acatgacggt ggcggagaac atcgccgtga cgccgcgcct gctcggctgg agcgaagacg
     1741 aagttcgcgc ccgcgtcgag gagctgctcg cgctcgtcaa cttgcccgtg gaaatggcgc
     1801 cgcggctgcc cgcggcgttg tcgggcgggc agcgccagcg cgtcggtgtc gcgcgcgcgc
     1861 tcgcggcgcg cccggcgatc atgctcatgg acgagccctt cggcgcgctc gatcccgtca
     1921 cgcgcgacag tctcggaggc gaatatcggc agctgcacga acgcatgggc ctcgtgaccg
     1981 tcatggtcac gcacgacgtg ctcgaggcgc tgctcctcgc cgatcgcatc gtcgtcatgc
     2041 gcgacggcgg catcgtcgcg agcggcacgc cgcgcgagat gatgaacgag cgaggcgacg
     2101 ccgaggttcg caggctcatg gacatgccgc gccgtcagat gacacgcgtc gcggccatgc
     2161 tcgacgacgc gcggcggcca tgaacgatcg cttcgcaagc gcgtgggccc tcttgcccgg
     2221 ctacttgagc cagcacgtcg cgctgagcgc cgccgccatg gcgctcggct tggtcgtgag
     2281 cctcgcgctc gtcgtcgccg cggtgcgcag cgaacgcgtg cggtggccgg tgctcgcgtt
     2341 cgcgagcctg attcagaccg tgcccagcct cgcactgctc gcgcttttct atccgctgct
     2401 gctcgcgatt tccgggctca tcgagagcgt cttcggcacc ggtttctcgg cgctggggtt
     2461 cctgccgtcg cttctcgcgc tcgcgctgta ctcgatgctc cccgtcattc gcaacggcgt
     2521 cgcgggaatt ctcaacatcg acgccgcggt catcgaggcg gcgcgcggcg tcggcatgac
     2581 cgactggcag cgtctgcggc gcgtcgaatt gccgctcgcc gcgccgatgc tgatggccgg
     2641 gatccgcacc gcggccgtgt gggtcatagg cgccgcgact ttatcgacgc ccgtgggcca
     2701 agtcagcctc ggcaactaca tcttctcggg attgcagctg cagaattggg ttgcggtgct
     2761 gttcggctgc gtggcggcag cggcgctcgc gctcgcggtc gaccagctgc tcggcttgat
     2821 cgaaacgggc ctcgcgcgtc gcagccgttc gcgcgtcgca gccggcgtcg ccgcactggc
     2881 cgtcggcatc ggcgccgccg cgctgccgtt cggcggcgac tccgccgccg cgtacgtcgt
     2941 gggcggcaag aacttctcgg agcagttcat actcgccgag ctcatgagcg cgcggatcgc
     3001 ggcgtcgggc ggcgccgcgg cgcggcgcac gggcctaggc tccgccgtcg tgttcgcggc
     3061 gctcgcgagc ggcgacatcg acgtatacgt cgactacagc ggcacgatct gggccaacgt
     3121 catgcggcgc acggacgtgc cgcggcgcga gccgatgctc gccgagatgc gggcgtggct
     3181 cgaagcggag cacggcatcg tgctgctcgg ctcgctgggc ttcgagaatg cctatggcct
     3241 cgcgatgcgg cgcgatcgag cggagcggct cggcatcgcg acgatcgccg acctcgcggc
     3301 gcacgctgcc gagctttcga tgggcggcga cctcgagttc ttttcccgcc ccgagtggca
     3361 agcgctcgag tcttcctatg gcctcgcgtt cgcgactcgc cgcgaattcg agccgacgtt
     3421 catgtaccgc gccgtgacgg acggcgaggt cgacgtgatc tccgcattca cgagcgatgg
     3481 gcgaatcgcg gcgaacgact tgctcgtgct cgccgaccca aagggcgcga t
//
LOCUS       DASSNN010000696         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_982236, whole genome shotgun sequence.
ACCESSION   DASSNN010000696 DASSNN010000000
VERSION     DASSNN010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982236"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFL84_13055"
     CDS             complement(<1..680)
                     /locus_tag="VFL84_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609601.1"
                     /translation="MARIAASRSRLVFFLTLGALAACSERPGPAPEPAANAPAAGAGA
                     TVFTGARLIVGDGTVLEDATFTVGPDNRFGIVGATAAVTVPAGATTVDLAGLTVMPAI
                     VDTHTHLSRDRAGLMADLKHRAYFGVSAAMSLGQDNTDDAYAMRREVIPGHALYRMAG
                     RGFTAPEPGRTDIPYWITTAEEGRAGVREQAARGVDIIKIWVDDRNGQYAKLAPELYT
                     AVIDEAHKA"
     gene            complement(765..1319)
                     /locus_tag="VFL84_13060"
     CDS             complement(765..1319)
                     /locus_tag="VFL84_13060"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7609602.1"
                     /translation="MPRRRTSEELLHLFRENWPDGYSPAEEVMLRLYHAALRHTEELQ
                     RFLARFDLTTLEFVTLRVLRREPPPHVMTPSALHETLVLSAGGMTKIVKQLEAKGLVS
                     RRPNEADKRSKLVALTPRGQAAIEAAQSAVRDFDFALLERTLTRGEQQRLAQLLRKLL
                     VSLEPRRASAAVGFAAESLLRAEV"
     gene            complement(1337..1729)
                     /locus_tag="VFL84_13065"
     CDS             complement(1337..1729)
                     /locus_tag="VFL84_13065"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609603.1"
                     /translation="MRSKYLALLLGAAAVAAPVYAHHSFTATYDEGKTIQIEGELVQF
                     MFRNPHAFVHVMAPDENGQMQRWGVEWGGAGALTGQGVTRDSLKPGDKVIITGNPGRN
                     PIDHRLRMRTLLRPSDGFGWGQRGETFD"
     gene            complement(1857..>2266)
                     /locus_tag="VFL84_13070"
     CDS             complement(1857..>2266)
                     /locus_tag="VFL84_13070"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609604.1"
                     /translation="RMSTRHAIFGAALGLASVVAHAHHSIAAVYDSSRERRLEAVVAE
                     FKFVNPHPFVIVTVDEDGAEQSWRLEMDNRFELAQVGMTDTTLKRGDRVIVTGSAGRT
                     EPRTLYILRLDRPADGFRYEQVGSRPRVSTVRR"
BASE COUNT          340 a          777 c          773 g          376 t
ORIGIN      
        1 gccttgtgcg cctcgtcgat gacggccgtg tacagctcgg gcgcgagctt cgcgtactgg
       61 ccgttccggt cgtcgaccca gatcttgatg atgtcgacgc cgcgcgcggc ttgctcgcgc
      121 acgccggcgc gtccttcctc cgccgtcgtg atccagtacg ggatatccgt gcggcccggc
      181 tcgggcgccg tgaagccgcg gcccgccatg cgatacagcg cgtggccggg gatcacttcg
      241 cggcgcatcg cgtacgcgtc gtccgtgttg tcctggccca agctcatggc ggcgctgacg
      301 ccgaaatacg cgcggtgctt gagatcggcc atgagccccg cgcggtcgcg cgacaggtgc
      361 gtatgggtgt cgacgatcgc gggcatcacg gtgagccctg cgaggtcgac ggtcgtcgcg
      421 ccggccggga ccgtaacggc cgcggtggcg ccgacgatgc cgaagcgatt gtcgggaccg
      481 accgtgaacg tcgcgtcctc gagcacggtt ccgtcgccga cgatgagccg agcgcccgtg
      541 aacacggtgg cgccggcgcc cgccgccggc gcgttggcgg ccggttcggg cgcggggccc
      601 ggccgctccg agcatgcggc cagcgcgccg agcgtgagaa agaaaacgag acgagaacgc
      661 gatgctgcga tccgcgccat ggctcactcc ccctatcctt tgcccgcgat cttaggccga
      721 tcggccggcg gccgccaatc gcgaacggcg actgcggaaa ggcgctagac ctcggcgcgc
      781 aaaagcgatt cggcggcgaa gccaacagcg gccgatgcgc ggcgcggctc gaggctcacg
      841 agcagcttgc gcagcaactg cgcgaggcgt tgctgctcgc cgcgcgtcag cgtgcgctcg
      901 agcagcgcga agtcgaagtc gcgcacggcg ctctgcgcgg cctcgatcgc ggcttgcccg
      961 cgcggcgtca gagcgacgag cttgctgcgc ttgtcggctt cgttcggacg ccgcgagacg
     1021 agaccctttg cttcgagctg cttcacgatc ttcgtcatgc cgccggccga gagcacgagc
     1081 gtctcgtgca gcgcgctcgg cgtcatgacg tgcggcggcg gctcgcgccg cagcacgcgc
     1141 agcgtcacga actcgagcgt cgtcagatcg aaccgcgcga ggaagcgctg cagctcttcg
     1201 gtgtgccgca acgccgcgtg gtagagccgc agcatgacct cttcggcggg ggaatagccg
     1261 tccggccagt tctcccggaa caggtgcagc agctcctccg aggtgcggcg gcgcggcatt
     1321 cgcgctgcgc ccccgctcag tcgaacgtct cgccgcgctg gccccagccg aagccgtcgg
     1381 acggccgcag cagcgtgcgc atgcgcaacc gatgatcgat cggattgcgg cccgggttgc
     1441 ccgtgatgat gaccttgtcg cccggtttca acgagtcgcg cgtgacgccc tgacccgtca
     1501 gcgcgcccgc gccgccccat tcgacgcccc agcgctgcat ctgcccgttc tcgtccggcg
     1561 ccatgacgtg cacgaacgcg tgcggattgc ggaacatgaa ctgcacgagc tcgccttcga
     1621 tctggatggt cttgccctcg tcgtacgtgg ccgtgaacga gtgatgcgcg tagaccggcg
     1681 ccgcgacggc ggcagcaccc aacagcaaag cgagatactt cgatctcatg attgcaccct
     1741 ccggggtggc gatgcattta atttactaga aaactaattc cgcggtcatt ttccggctca
     1801 acttttcttc gcgctcgggc tttcttgcga ctcgcggatc tcgagcgggc ccgtcgtcag
     1861 cggcgcacgg tgctgacgcg cggccgcgag ccgacttgct cgtaacggaa gccgtcggcg
     1921 ggacggtcta gcctcaagat atagagcgtg cgcggctccg tgcgccctgc actgcccgtc
     1981 acgatcacgc gatcgccgcg cttcaaggtc gtatccgtca tgccgacttg ggcgagctcg
     2041 aaccgattgt ccatctcgag ccgccaagat tgctcggcgc cgtcttcgtc gacggtgacg
     2101 atcacgaacg ggtgcggatt cacgaacttg aactcggcga cgacggcttc gagacgccgc
     2161 tcgcgcgagc tgtcgtaaac ggccgcgatc gaatggtgcg cgtgcgctac gacgctcgcg
     2221 agcccgagcg cggcgccgaa gatggcgtgt cgcgtgctca tccgta
//
LOCUS       DASSNN010000697         3813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_858856, whole genome shotgun sequence.
ACCESSION   DASSNN010000697 DASSNN010000000
VERSION     DASSNN010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858856"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1725
                     /gene="ligD"
                     /locus_tag="VFL84_13075"
     CDS             <1..1725
                     /gene="ligD"
                     /locus_tag="VFL84_13075"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HET7609605.1"
                     /translation="TFPEIAEIAAALPFSQFIIDAEVVVLDARGIPSFSLLQKRGRLT
                     RRADVARAALELPASIYAFDLLAFEGLDLRALPLVERKAALRALLPVSGAFRYSEHFE
                     AQGEALFDEAERLGLEGIIGKRAASAYVSKRSTDWIKVNAAKSDDFVVVGYLPPKQGG
                     KGFGALLLAQYRDGVLTYAGRAGSGFAARDFAALEPLLAAKPRAAPPPKAELARGAVW
                     LADGPVVQVKFKQRTPDGMLRQPVYLRTRDDKKPEECVWQTEQAAPELDAEPAAPAVE
                     PPERPSVAFTNLDKVFWPADGYTKGDMIAYYEGVAAWLLPYLKDRPVVLTRYPDGIDG
                     KSFFQKDAPVYAPSWLRLETMWSEHAEREIRYFVLDDLESLLYVANMGTIPLHVWSSR
                     VAALERPDWCILDLDPKGAPLKDVVKIALHLHELCEDVGLANFVKTSGSTGLHVLIPL
                     GARYTYEQSRTLAELLARFAAKSLPEIATIVRSVSDRDGKVYVDFLQNGHGRLLVSPF
                     CVRPLPGAPVSMPLEWSEINGRLKLERFNIRSAGKWLAKHGDPLAPVLTASSDMLAAL
                     DSLTRRLA"
     gene            1796..2515
                     /locus_tag="VFL84_13080"
     CDS             1796..2515
                     /locus_tag="VFL84_13080"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HET7609606.1"
                     /translation="MRKPVTLIAGEFGSPHFGTSPHLEEAVRLARKEAPQALYIGAAS
                     GDDETFGTALCALIGAAGASDVFWPKLTKRPRDKSVARKALARVDFVFVGGGDVEAGM
                     DVLRRAQLIAELRAAAARGVVFAGMSAGAIMLGERWVRWPRADATDDEAETYECLGLA
                     PCALDTHGEGDRWGEARSFAAVRARELKRKARAFGIPSGGALVVRGGGKMHARGKPVP
                     VFVASPRGAATIERTLPAEKG"
     gene            2512..3750
                     /locus_tag="VFL84_13085"
     CDS             2512..3750
                     /locus_tag="VFL84_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609607.1"
                     /translation="MSYSTAHWRRVVDETRRMVAEVPLYRDRPQPPADPAEVDAWLSQ
                     VPPVGKRELRRGFPKALVREHQDLSAAMRNEQVTLLATSGTTSDRLQVIWEWSWWDPQ
                     EREAMRLNARIAQAMARSDYREAVLTTPACGAGTCHFGSQTAAERSIDGILFFNESAD
                     PTYWTDGECDRMLAEWREFAPRGVEADPAYLAIIARAALARGVRLPPLEFITLTYETT
                     TRAMRRDIGRAFDAPVFQLYGATEAGVLFMECEHGLLHPNERHSHVDLVPLPGAGRLA
                     RVLVTTLGRAWMPLLRYDVGDIVRVAESRDCACGLKSDGPLLTRVEGRQSDCLAIGGE
                     TVTPLMLDDAIHAALGPSATIEQWQLAGDDLLVVDPKSGDSAAQSAAAVGALLRRAIR
                     GERVAAIAPEASGKYRLVKR"
BASE COUNT          583 a         1306 c         1333 g          591 t
ORIGIN      
        1 acgttcccgg agatcgccga gatcgcggcc gcgctgccgt tctcgcaatt catcatcgac
       61 gccgaagtcg tcgtgctcga cgcgcgcggt atcccaagct tctcgttgct gcagaagcgc
      121 ggccgcttga cgcgccgcgc cgacgtcgcg cgcgccgcgc tcgagctgcc cgcaagcatc
      181 tacgcgttcg atttgctcgc gttcgaaggc ctcgacctgc gcgcgttgcc gctcgtcgag
      241 cgcaaggcgg cgttgcgagc gctgcttccc gtctcgggcg cgtttcgtta ctccgagcat
      301 ttcgaagcgc aaggcgaggc gttgttcgac gaagccgagc ggctcggcct cgagggcatc
      361 atcggcaagc gcgcggcatc ggcgtacgtg agcaagcgct cgacggattg gatcaaggtc
      421 aacgccgcaa agtccgacga cttcgtcgtc gtcggctact tgccgccgaa gcaaggcggc
      481 aaagggttcg gtgcgctgct gctcgcgcag taccgggatg gcgtgctgac gtacgcgggg
      541 cgcgccggca gcggcttcgc cgcacgcgac ttcgccgcgc tcgagccgtt gctcgcagcg
      601 aagccgcgcg ccgcgccgcc gccgaaagcg gagctcgcgc gcggcgcggt gtggctcgcg
      661 gacgggcccg tcgtgcaggt caagttcaag cagcgcacgc ctgacggcat gctgcgccag
      721 ccggtttatc tgcgcacgcg cgacgacaag aagccggagg agtgcgtctg gcaaacggag
      781 caggcggcgc cggaactcga cgccgagccg gcggcgccgg ccgtcgagcc gcccgagcgg
      841 ccgagcgtcg cgttcacgaa cctcgacaag gtgttctggc ccgccgacgg ctacacgaag
      901 ggcgacatga tcgcctacta cgaaggtgtg gccgcgtggc tgctgccgta tttgaaggat
      961 cggcccgtcg tgctgacgcg ctatcccgac ggcatcgacg gcaagtcgtt cttccagaaa
     1021 gacgcgcccg tctatgcgcc gagctggctc aggctcgaga cgatgtggag cgagcacgcc
     1081 gagcgcgaga tccgctactt cgtgctcgac gacctcgagt cgctgctcta cgtcgcgaac
     1141 atgggcacga tcccgctgca cgtctggtcg agccgcgtcg cggcgctcga gcgtcccgac
     1201 tggtgcatcc tcgatctcga tccgaaaggc gcgccgctca aggacgtcgt caagatcgcg
     1261 ctgcacctgc acgagctctg cgaggacgtg gggctcgcga acttcgtcaa gacgagcggc
     1321 tcgacgggcc tgcacgtgct gatcccgctc ggcgcgcgct acacgtacga gcagtcgcgc
     1381 acgctcgcgg agctgctcgc gcgcttcgcg gcgaagtcgc tgccggagat cgcgacgatc
     1441 gtgcgctcgg tctcggaccg cgacggcaag gtctacgtcg acttcttgca gaacggccac
     1501 ggccgcttgc tcgtgtcgcc gttctgcgtg cggccgctgc ccggcgcgcc cgtctcgatg
     1561 ccgctcgagt ggtcggagat caacggccgc ctcaagctcg agcgcttcaa catccgctcg
     1621 gcaggcaagt ggctcgccaa gcacggcgat ccgctggcgc ccgtcctgac ggcgtcctcc
     1681 gacatgctgg ccgccctcga ctcgctgacc cgccgcctcg cctagcgaaa aggggacaga
     1741 ccccattttc atttttcgcg gaaaatagat ctgtcccctt tttgaaggag ctcgattgcg
     1801 caagcctgtc acgctgatcg ccggcgagtt cggcagccct catttcggca cgagccctca
     1861 tctcgaagaa gccgtgcgac tcgcgcgcaa ggaggcgccg caggcgctgt atatcggcgc
     1921 tgcaagcgga gacgacgaga cgttcggcac cgcgctctgc gcgctgatcg gcgccgccgg
     1981 cgcgtccgac gtcttctggc cgaagctcac gaagcggccg cgcgacaagt cggtcgcgcg
     2041 caaagcgctc gcgcgcgtcg acttcgtatt cgtcggcggc ggcgacgtcg aggccggcat
     2101 ggatgtcttg cggcgcgcgc aactcatcgc cgagcttcgc gctgcggcgg cccgcggtgt
     2161 cgtatttgcc gggatgtccg ccggtgcgat catgctcggc gaacgctggg tgcgctggcc
     2221 gcgcgccgac gccaccgacg acgaggcgga gacgtacgag tgtcttggcc tagcgccatg
     2281 cgcgctcgat acgcacggcg aaggcgaccg gtggggcgag gcgcggtcgt tcgcggccgt
     2341 gcgcgcgcgc gagctgaagc gcaaagccag ggcgttcgga atcccgagcg gcggcgcgct
     2401 ggtcgtccgc gggggtggca aaatgcacgc acgagggaag ccggtacccg tgttcgtggc
     2461 cagtccgcgt ggggccgcga cgatagaacg gacgttaccg gccgagaagg gatgagctac
     2521 tcgacagcac attggcggcg ggtcgtcgac gagacgcgcc gcatggtggc cgaggtgccg
     2581 ctgtaccgcg atcggccgca accgccggcc gacccggccg aggtcgacgc gtggctctcg
     2641 caggtgccgc ccgtcggcaa gcgcgagctg cggcgcggct ttccgaaagc gctcgtgcgc
     2701 gagcaccagg atctttccgc cgcgatgcgc aacgagcaag tcacattgct cgcgacgtcg
     2761 ggcacgacct ccgatcggct gcaagtcatc tgggaatggt cgtggtggga cccgcaggag
     2821 cgcgaggcca tgcgcttgaa cgcgcgcatc gcgcaggcga tggctcgctc cgattatcgc
     2881 gaagccgtgc tgacgacgcc ggcctgcggc gcgggtacct gtcatttcgg cagccagacc
     2941 gcggccgagc gcagcatcga cggcatcctg tttttcaacg agagcgccga cccaacgtat
     3001 tggaccgacg gcgaatgcga ccgcatgctc gccgagtggc gcgagttcgc gccgcgcggc
     3061 gtcgaggccg acccggcgta cctcgccatc atcgcgcgcg cggcgctcgc gcgcggcgtg
     3121 cgcttgccgc cgctcgagtt catcacgctc acatacgaga cgacgacgcg agcgatgcgc
     3181 cgcgatatcg gccgcgcgtt cgacgcgccg gtgttccaac tgtacggcgc gaccgaggcc
     3241 ggcgtcttgt tcatggagtg cgagcacggg ctgctgcacc cgaacgagcg gcacagtcac
     3301 gtcgatctcg tgccgctccc cggggcgggg cggctcgcgc gcgtgctcgt cacgacgctc
     3361 ggacgagcgt ggatgccgct gctgcgctac gacgtcggcg acatcgtgcg cgtcgcggaa
     3421 tcgcgcgact gcgcgtgcgg cctcaagagc gacgggcccc tgctcacgcg cgtcgaaggc
     3481 cgccagagcg attgcttggc aataggcggt gaaaccgtga cgccgctgat gctcgacgac
     3541 gcgatccacg cggctctcgg gccctcggcc acgatcgagc agtggcagct cgccggcgac
     3601 gacctgctcg tcgtcgatcc gaagagcggc gacagcgcgg cgcagtcggc cgccgccgtc
     3661 ggtgcgctgc tgcggcgggc gattcgcgga gagcgcgtcg cggcgatcgc gcccgaagca
     3721 tccggaaaat accgcctcgt caaacgctga tgcgaccctc cgggtttccg ctgctcgaga
     3781 acggctacga cggcatgccg ttcgtctacc tcg
//
LOCUS       DASSNN010000698         3884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1132014, whole genome shotgun sequence.
ACCESSION   DASSNN010000698 DASSNN010000000
VERSION     DASSNN010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132014"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            411..1841
                     /locus_tag="VFL84_13090"
     CDS             411..1841
                     /locus_tag="VFL84_13090"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HET7609608.1"
                     /translation="MRGSTVYDAPTLFEVYREQLGKPVSADRARAIVAALLAKYEADG
                     YSRPQAKLDDALLEAGVLRIDIREPRIGEVRVNGDPGPHLERLETLGTKLRDDVPITQ
                     AELASTLRQMRSLPGLTLQATTARDDASANVYRLDLDTQFDRMTGVVRLSNRGTDEAG
                     PVFLLGQVMFNGLLGGRTNLGAVFGGATDYDEYHGLGLLAQVGVGASGGGLSFSGFRS
                     RSDPHEPLVDRDDDYLRDRASISFARPLPGFERANLVLAAALDLDDLEILRAGERLRD
                     ERLRMLSVNPRWSWRHERGAQYLVGLEVVKGLDAMGSGLTALDIEEDARRADFTLTRM
                     TFTRLSRFGESWSVRFDALAQQTPHVLPYAERFKIGGERLGRGFEVAEIAGDEGAGAK
                     VELRRNLDAAPALLRGASLYGFYDLAAAFKNNQPGRESAATAGFGLAVQHRRVSSIIE
                     LAKPLTHPDVEGRKELALFAEVALLL"
     gene            1866..2855
                     /locus_tag="VFL84_13095"
     CDS             1866..2855
                     /locus_tag="VFL84_13095"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7609609.1"
                     /translation="MKLASVHYRGRDLVVTEIQEGELVGISSLPGRPATQPVDMLDVV
                     RGGEPLKQALAASLDKIRGDASVARIPIADVRWYPPVRRPGKICAVAMNNSASNERKI
                     SAPDHPAFFLKPSSCLVGHREPIRIRRYYGSVHPEPELAVVIGRAARDVPATNALDAV
                     YGYTIFNDITGNGMRAEDRFHYWAVYAKKDNPSETERVEQHLSYAGRYKGTDTFGAMG
                     PWLVTRDEIANPDDLSVTCRVGGELVADDSTRYYNYKVAEVVSFISQFQTLLPGDVIA
                     LGTAFKPGQGRKSIHTANFQTVAGPVEIAIEGLGVQQNPILIEDKELGAWRLP"
     gene            complement(2865..3185)
                     /locus_tag="VFL84_13100"
     CDS             complement(2865..3185)
                     /locus_tag="VFL84_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609610.1"
                     /translation="MHFRPVILGLAALWLVLFVGSFVSLQATGTDGGSESGLARTAVF
                     LTWQVLAFAVAMLGAFTTRLAAARGAERVKAFGYVPLALSVFLVGSFIALVAFRVWVL
                     PLFN"
     gene            complement(3196..>3884)
                     /locus_tag="VFL84_13105"
     CDS             complement(3196..>3884)
                     /locus_tag="VFL84_13105"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HET7609611.1"
                     /translation="EWPDTIHDFGGFPDELYRLRYPAPGSPQLARDIVARLAAADVSA
                     RLAPSRGLDHGAWIPLSLLLPRADVPVLQVSINSRRSPEQHFALGRALRSLRDSGTLV
                     LGSGGATHNLALYAHARGRDDDSAPPEWVEAFNEWTAGAVAGRRFDDLFRYAERAPFA
                     AQNHPTPEHYLPLFVTLGTAHDDEPGVRIHSSYDRGLLSLDAYAFGLVPPAPREIRVR
                     SAAPGPAVLH"
BASE COUNT          680 a         1328 c         1315 g          561 t
ORIGIN      
        1 gcgcgcagag ctcgacccgg cgtatgaagt cgcggtcgcc gcgacgagca cgagtgccgg
       61 agcgagtagg aggaggcgcg gcgagatccc ctgcgagtcc gtcttcttct tcatgaccga
      121 cactccatgt cgtcaacgag cgacagaata gacgcgggcg agcggcaatg ctgtgacgac
      181 actcacggcg gcgtccagcg agattgtcca taatccgcaa ccatcggccc tctgctccgc
      241 tggatcgaat gcgcccggac gagacgcgat gcgacctacg aaagcattgc ggcggtgacc
      301 gaccgccaag ccgagcgaga gcgctccgag ttggcataat cgtccttgcg tttgaactaa
      361 tctgcgcgag tgccgcagcc gcggccacgc cgaatctcgt ttccgtcgtc gtgcgcggct
      421 ccacggtcta cgacgcgccg acgctgttcg aggtctatcg cgagcaactc ggcaagcccg
      481 tgagcgccga tcgtgcacgt gcgatcgtgg ccgcgctgct cgcgaagtac gaggcggacg
      541 gttactcgcg gccgcaagcc aagctcgacg acgcgttgct cgaggccgga gtgctacgaa
      601 tcgacatccg cgagccgcgc atcggcgaag tccgcgtcaa cggcgatccc gggccgcacc
      661 tcgagcggct cgagacgctc ggcacgaagc tgcgcgacga cgttccaatc acgcaagcgg
      721 aattagcgtc gacgttgcgg cagatgcgct cgctgccggg attgacgctg caggcgacga
      781 cagcccgcga cgacgcgagc gcgaacgtct atcgactcga tctcgacacg caattcgacc
      841 gcatgacggg cgtcgtgcgg ttgagcaacc gcggcaccga cgaggcgggg cccgtattcc
      901 tgctcggcca ggtcatgttc aacgggctac tcggcggccg gacgaacttg ggcgcggtct
      961 tcggcggcgc caccgactac gacgaatatc acgggctcgg gttgctcgcc caagtcggcg
     1021 tcggcgcgag cggcggcggg ctctcgttca gcggctttcg ctcgcgctcg gatccgcacg
     1081 agccgctcgt cgaccgcgac gacgactatc tgcgcgatcg tgcctcgatc tccttcgcgc
     1141 ggccgttgcc cggcttcgag cgagcgaacc tcgtgctcgc ggccgcgctc gacctcgacg
     1201 atctcgaaat tctgcgcgcg ggcgagcggc tgcgcgacga gcggctccgc atgctctccg
     1261 tcaacccgcg gtggagctgg cggcacgagc gcggcgcgca gtacttggtc ggcctcgagg
     1321 tcgtgaaagg gttagacgcg atgggcagcg ggctgacggc gctcgacatc gaggaagatg
     1381 cccgacgcgc ggatttcacg ctgacgcgca tgacgttcac gcgcctcagc cgcttcggcg
     1441 agagctggtc cgtgcgcttc gatgcgctcg cccagcaaac gccgcacgtg cttccgtacg
     1501 ccgagcggtt caagatcggc ggcgagcggc tcggccgcgg cttcgaagtc gccgagatcg
     1561 caggcgacga aggcgccggc gcgaaagtcg agctgcgccg caacctcgac gccgcgcccg
     1621 cgctgctccg cggcgcctcg ctgtacggct tctacgacct ggccgcggcg ttcaagaaca
     1681 accagccggg ccgcgagtcg gccgcgacgg cgggcttcgg cctcgccgtg cagcaccgcc
     1741 gcgtgtcgag catcatcgag ctcgcaaaac cgctgacaca ccctgacgtc gagggccgta
     1801 aggagctcgc gctgttcgcg gaagtcgcgc tgctgctcta acgcgatgga gtaagatcgg
     1861 cggccatgaa attggccagc gtccattacc gcggccgcga cttggtcgtc acggagatcc
     1921 aggaaggcga gctcgtcggc atctcgtcgt tgcccggccg cccggcgacg cagcccgtcg
     1981 acatgctcga cgtcgtccgc ggcggcgagc cgctgaagca ggcgctcgcc gcttcgctcg
     2041 acaagatacg cggcgacgcg tccgtcgcgc gcatccctat cgcggacgtt cgttggtatc
     2101 cgcccgtgcg ccgcccgggc aagatctgcg cggtcgcgat gaacaactcg gcgagcaacg
     2161 agcgcaagat cagcgcgccc gaccatccgg cgttcttctt gaagccgtca tcgtgtctcg
     2221 tcgggcatcg agagccgatt cgcattcgcc gctactacgg cagcgtgcat cccgagcccg
     2281 agctcgccgt cgtcatcggc agggccgcgc gcgacgtgcc cgcgacgaac gcgctcgacg
     2341 ccgtctacgg ctacacgatc ttcaacgaca ttaccggcaa cggcatgcgc gcagaggatc
     2401 ggtttcatta ctgggccgtc tacgcgaaga aagacaaccc gagcgagacg gagcgcgtcg
     2461 agcagcatct ttcgtacgcc ggccgctaca agggcaccga cactttcggt gcgatgggcc
     2521 cgtggctcgt cacgcgagac gagatcgcga accccgacga tttgagcgtg acttgcaggg
     2581 tcggcggcga gctcgtggcc gacgacagca cgcgctacta caactacaag gtcgccgagg
     2641 tcgtgagctt catcagccag ttccagacgc tgctgcccgg cgacgtgatc gcgctcggca
     2701 ccgcgttcaa gccggggcaa ggccgcaaat cgatccacac ggcgaacttc cagaccgttg
     2761 cgggccccgt cgagatcgcc atcgaagggc tcggagtgca gcagaacccg atcctgatcg
     2821 aggacaaaga gctcggcgca tggcgcctgc cttgagcgag ccggctagtt gaacagcggc
     2881 aggacccaga cccgaaacgc gacgagcgcg atgaacgagc cgacgaggaa cacgctgagc
     2941 gcgagcggca catagccgaa cgccttgacg cgttcggcgc cgcgggccgc cgcgagccgc
     3001 gtcgtgaaag cgccgagcat cgccacggcg aatgcgagga cctgccacgt cagaaacacg
     3061 gccgtgcgcg cgagcccgct ttccgagccg ccgtccgtcc ccgtcgcttg cagcgacacg
     3121 aacgaaccca cgaacagcac cagccacagc gcggctaatc ccaagatgac cggccggaag
     3181 tgcatgggcc gctcgctaat gcagaaccgc ggggccgggc gcggccgagc ggacgcgaat
     3241 ctcgcggggc gcgggcggca cgagaccgaa cgcatacgcg tcgagcgaca gcaggccgcg
     3301 atcgtagctc gagtggatgc gcacgccggg ctcgtcgtcg tgtgcggtgc cgagcgtcac
     3361 gaacagcggc agatagtgct cgggcgtcgg atgattctgc gccgcaaacg gcgcgcgctc
     3421 ggcataacga aacagatcgt cgaagcgccg ccccgctacg gcgccggccg tccactcgtt
     3481 gaacgcctcg acccactcgg gcggcgcgct gtcgtcgtct ctgccgcgcg cgtgcgcgta
     3541 cagcgcgaga ttgtgcgtgg cgccgcccga tcccaagacc agcgttccgc tgtcgcgcag
     3601 cgagcgcagc gctcgaccca acgcgaagtg ttgttccggc gagcggcggc tgttgatcga
     3661 gacctgcagc accggcacat cggcgcgcgg cagcaataac gacagcggta tccacgcgcc
     3721 gtggtcgagc ccgcggctcg gcgcgagccg cgcggacacg tcggccgccg ctagacgagc
     3781 gacgatgtcg cgcgcgagct gtgggcttcc gggcgcgggg tagcgcaggc gatagagctc
     3841 gtccggaaag ccgccgaagt cgtgaatcgt gtcgggccac tccg
//
LOCUS       DASSNN010000699         4972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_859169, whole genome shotgun sequence.
ACCESSION   DASSNN010000699 DASSNN010000000
VERSION     DASSNN010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859169"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VFL84_13110"
     CDS             <1..687
                     /locus_tag="VFL84_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HET7609612.1"
                     /translation="INSTNVGEMLRQIGVAPFVEAETAEITASLYWPGPPSAAWLDHI
                     AGDLALRAEKGSLVDVAPGGAGRAAGLLSISALPRRLALDFRDVFNRGFVFDDITADF
                     VIVDGNAYTDNLKLTGPVAEVGLIGRTGLRDRDYRQQVVVTAEPGKVLPTVGALLAGP
                     QVAAALLIFTRIFKKPLGGIGRASYCVTGSWSEPVVERLTDEQLEEGAVCAELPPSAA
                     LLKPQEVAAR"
     gene            684..1496
                     /locus_tag="VFL84_13115"
     CDS             684..1496
                     /locus_tag="VFL84_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET7609613.1"
                     /translation="MKVAAVQMTSTRDVAANLREAGRLVADAARQGAQLVVLPENFSF
                     LGATDADRVAAVERFGDGPAQRFLAETAESLGLWIVGGTIPIRDGAADRASSRSLLVG
                     PDGRVAAHYDKIHLFDVDIPGREKERYSESATTLAGTRVVAATTPLARIGMTVCYDIR
                     FPALFHRLSVLGTDIVVVPAAFTVPTGEVHWLPLLQARAIESLVYVVAAGQWGEHAGG
                     RKTYGHSLILGPWGELMAELPAGPGVVCADLDMIVLTDLRRRFPTVQHRREL"
     gene            1522..3000
                     /gene="tldD"
                     /locus_tag="VFL84_13120"
     CDS             1522..3000
                     /gene="tldD"
                     /locus_tag="VFL84_13120"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HET7609614.1"
                     /translation="MRKPSKQVAPAPVDSFAIARELLLEGQGLELAALERALSAAMVR
                     KLDYVDLYLQLTRYETWTVEDGIVKEGAYSVDQGIGVRAVSGERTGFAYSDQLDETAL
                     TDAVVAARGIARTKGGGSQKIPARVASHRPLYPSGDPLRSMTDSDKVALLAELDRRVR
                     AMDSRVVQVIGSLSGVYEVVLVQATDGTLAADVRPLVRLNVSVIMEKDGRREQGYAGG
                     GGRGDYTLVTGNGRPLELAAEAVRTAAVNLSAVPSPAGTMPVVLGSGWPGILLHEAIG
                     HGLEGDFNRKGTSAFTGRIGERVATDQCTVVDDGTLVGRRGSLTVDDEGTPTSCNTLI
                     ESGILKGYMFDKLNARLMGVKSTGNGRRESFAHTPMPRMTNTYMLPGKYDPEEIIASV
                     DRGIYAPNFGGGQVDITSGKFVFSASEAYIIENGKKTVPVKGAMLIGSGPEVLTRVSM
                     VGNDLKLDAGLGTCGKDGQSVPVGVGQPTIRIDSLTVGGTQA"
     gene            3093..3266
                     /locus_tag="VFL84_13125"
     CDS             3093..3266
                     /locus_tag="VFL84_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609615.1"
                     /translation="MVIDFRATLTEADFKRPLLDAAEDRLVREARGDVDLAVEGLRDS
                     LLSRLVRLFMGVR"
     gene            3277..3507
                     /locus_tag="VFL84_13130"
     CDS             3277..3507
                     /locus_tag="VFL84_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609616.1"
                     /translation="MWLSKPVYEALPYYYVALGAAALIARLYVDWWYWPAICLAVGVL
                     SIAAGLFVLVKRLKSRRSESRASRRARRAALP"
     gene            complement(3473..4540)
                     /locus_tag="VFL84_13135"
     CDS             complement(3473..4540)
                     /locus_tag="VFL84_13135"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7609617.1"
                     /translation="MLVEFGFKPFRKRKVELLSMIKDFMVEQYAAGKKILLVIDEAQN
                     LSRKVLEEVRLLSGLELQKEKLLRIIIAGQPELSHKLDSPRLQQLTQRVRLRFHLGAL
                     SKRETHEYITHRLEVSGAKGRRIFEPAACDLVFRYAGGVPRLVNVLCDTAMLCAFAEE
                     KTVIDEALVKAAVEELQWVEYAERLRTQERGMATGRFANVSRPLARLEVLYRDQLVGE
                     YRLAPGKTIIGRTPDNDVQIESRFISRHHAQVVSDQAHSFVEDLNSTNGVFIRSHRVK
                     HQTLSDGDIIQIGEHKLLYRDMRGASPPLLPPEDDDEPDDFDDDDDFDDEERDDEERD
                     EDDDADDADELKEEQLDERGG"
     gene            complement(4691..>4972)
                     /locus_tag="VFL84_13140"
     CDS             complement(4691..>4972)
                     /locus_tag="VFL84_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase"
                     /protein_id="HET7609618.1"
                     /translation="DQKIGWKSVSGDAPNAGTVRFEPIGPDRTLVRLVMAYEPQGATE
                     NVGDALGLLSTRVEKSVKQFKKYIESRGVEDGAWRGEVHDSKPQPKRPH"
BASE COUNT          757 a         1689 c         1675 g          851 t
ORIGIN      
        1 atcaactcga cgaacgtcgg cgagatgctg cggcaaatcg gcgtggcgcc gttcgtcgaa
       61 gcggagaccg ccgagatcac cgcgagcctc tattggcccg ggccgccttc ggccgcgtgg
      121 ctcgatcaca tcgcgggcga cctcgcgctg cgcgccgaga agggcagcct cgtcgacgtc
      181 gcgccgggcg gtgccgggcg cgccgcgggc cttttgagca tcagcgcgct gccgcgccgg
      241 ctcgcgctcg atttccgcga cgtgttcaac cgcggcttcg tgttcgacga catcacggcc
      301 gacttcgtca tcgtcgacgg caacgcctac acggacaacc tgaagctgac cggccccgtc
      361 gccgaggtcg gcctcatcgg ccgcacggga ctccgcgatc gcgactatcg gcagcaagtc
      421 gtcgtgacgg cggagcccgg caaggtgctg ccgaccgtcg gcgcgctgct cgcgggtccg
      481 caggtcgcgg ccgcgctgct gatcttcacg cgcatcttca agaagccgct cggcggcatc
      541 gggcgcgcgt cgtactgcgt gacgggcagc tggtcggagc ccgtcgtcga gcggctgacc
      601 gacgagcagc tcgaggaggg cgccgtctgc gccgagctcc caccgagcgc ggcgctcttg
      661 aagccgcagg aggtcgccgc gcgatgaaag tagccgccgt gcagatgacg agcacgcgcg
      721 acgtcgccgc gaacttgcgc gaggcgggcc ggctcgtcgc cgatgccgcg cggcagggcg
      781 cgcagctcgt cgtgctgccc gaaaactttt cgttcctcgg cgcgacggac gccgatcgcg
      841 tcgctgccgt cgagcgcttc ggcgacggcc ccgcgcaacg gtttctcgcc gagacggccg
      901 agagcctcgg tctctggatc gtcggcggca cgattccaat ccgtgacggc gccgccgatc
      961 gcgcgtcgtc gcgctcgctg ctcgtcgggc ccgacggccg agtggccgcg cactacgaca
     1021 agattcatct cttcgacgtc gacatcccgg gccgcgaaaa ggagcgctac agcgagtccg
     1081 cgacgacgct cgccggcacg cgcgtcgtcg cggcgacgac gccgctcgcg cgcatcggca
     1141 tgacggtctg ctacgacatc cgcttcccgg cgctctttca tcgcttgagc gtgctcggca
     1201 ccgacatcgt cgtcgtgccc gcagcgttca cggtgccgac cggcgaggtt cattggctgc
     1261 cgctcttgca agcgcgcgcg atcgagtcgc tcgtctacgt cgtcgccgcg ggtcagtggg
     1321 gcgagcacgc gggcggccgc aagacctatg gccactcgct gatcctcggt ccgtggggcg
     1381 agctgatggc cgaattgccc gccggtcctg gcgtcgtttg cgccgatctc gatatgatcg
     1441 tcctcacgga cctgcgccgc cgctttccga ccgtccaaca tcggcgcgag ctctaagccc
     1501 gggccttcta ggagagttgt agtgcggaaa ccgtcgaagc aagtagcgcc ggcccccgtc
     1561 gattcgttcg cgatcgcgcg cgagctgctg ctcgagggcc aaggcctcga gctcgccgcg
     1621 ctcgagcgag cgctgtcggc cgcgatggtg cgcaagctcg actacgtcga tctgtatctg
     1681 cagctcacgc gctacgagac ctggaccgtc gaggacggca tcgtcaaaga gggcgcgtac
     1741 agcgtcgacc agggcatcgg cgtgcgggcc gtctcgggcg agcgcacggg cttcgcctac
     1801 tccgaccagc tcgacgagac ggcgctgacc gacgccgtcg tcgccgcgcg cggcatcgcg
     1861 cgcacgaaag gcggcggctc gcagaagatc cccgcgcgcg tcgcgagcca tcggccgctc
     1921 tatccctccg gcgatccgct gcgcagcatg accgactccg acaaggtcgc gctgctcgcg
     1981 gagctcgacc ggcgcgtccg cgccatggac agccgcgtcg tgcaagtgat cggcagcctc
     2041 tcgggcgtct acgaggtcgt gctcgtccaa gccaccgacg gcacgctggc cgcggacgtg
     2101 cggccgctcg tgcgcctcaa cgtctccgtg atcatggaaa aggacggccg gcgcgagcag
     2161 gggtatgccg gcggcggcgg ccgcggcgac tacacgctcg tgaccggcaa cggccggccg
     2221 ctcgagctcg cggccgaggc cgtgcgcacg gcggccgtca acctatcggc cgtcccatcc
     2281 cctgcgggca cgatgcccgt cgtgctcggc tcgggctggc ccggcatctt gctgcacgaa
     2341 gcgatcggcc acggcctcga gggcgacttc aatcgcaagg gaacgtcggc gttcacgggc
     2401 cgcatcggcg agcgcgtcgc gaccgatcaa tgcacggtcg tcgacgacgg caccttggtg
     2461 ggccggcgcg gctcgctgac cgtcgacgac gaaggcacgc cgacgagctg caacacgctg
     2521 atcgagagcg gcatcttgaa gggctacatg ttcgacaagc tgaacgcgcg cctcatgggc
     2581 gtgaagtcga ccggcaacgg gcggcgcgag tcgttcgcgc atacgccgat gccgcgcatg
     2641 acgaacacct acatgctgcc gggaaaatac gatcccgagg agatcatcgc gtcggtcgac
     2701 aggggcattt acgcgccgaa cttcggcggc ggccaggtcg acatcacgag cggcaagttc
     2761 gtgttctcgg cgagcgaggc gtacatcatc gagaacggca agaagacggt gcccgtcaaa
     2821 ggcgcgatgc tgatcggcag cgggcccgag gtgctgacgc gcgtgtcgat ggtcggcaac
     2881 gatttgaagc tcgatgcggg cctcggcacg tgcggcaagg acgggcagtc cgtgcccgtc
     2941 ggcgtgggcc agccgacgat tcggatcgac tcgctgaccg ttggcggcac gcaggcgtga
     3001 gttacgtcaa accgcgctcg tgaatggctt cactgcaaac ggagccgcgg cgccctagtc
     3061 ttagcgaagt cgtttttttt gagagccgac agatggtcat cgatttccgc gcgacgctga
     3121 ccgaagccga cttcaagcgg ccgctgctcg atgcggccga agatcggctc gttcgcgagg
     3181 cccgcggcga cgtcgacctc gccgtcgagg gccttcggga ctccctgctg agccggctcg
     3241 tgcgcctctt catgggtgtc cgctagactg ccacccatgt ggttgtcgaa accggtctac
     3301 gaggcgctgc cgtactacta cgtggcgctc ggcgcggccg cgttgatcgc gcgtctctac
     3361 gtcgactggt ggtattggcc cgcgatctgc ctcgccgtcg gagtgctctc catcgcggcg
     3421 ggtctcttcg tcttggtcaa gcgcctgaag tcccggcgct ccgagtcgcg cgctagccgc
     3481 cgcgctcgtc gagctgctct tccttgagct cgtcggcgtc gtcggcgtcg tcgtcctcgt
     3541 cgcgctcttc gtcgtcgcgc tcctcgtcgt cgaagtcatc gtcgtcgtcg aaatcgtccg
     3601 gctcgtcgtc gtcctctggc ggaagcaacg gcgggcttgc gccgcgcatg tcgcgataga
     3661 gcagcttgtg ctcgccgatc tggatgatgt cgccgtccga tagcgtctgg tgcttgacgc
     3721 ggtgtgagcg aatgaagacg ccgttggtgc tgttcaagtc ctcgacgaac gaatgcgctt
     3781 ggtcggagac gacctgcgcg tggtgccgcg agatgaagcg gctctcgatc tgcacgtcgt
     3841 tatcgggcgt gcgcccgatg atcgtcttgc cgggagcgag gcgatattcg ccgacgagct
     3901 gatcgcggta cagcacctcg aggcgcgcga gcggccggct cacgttcgcg aaccgcccgg
     3961 tggccatgcc gcgctcctgg gtgcgcagcc gctccgcgta ttcgacccac tgcagctcct
     4021 cgacggccgc cttgacgagc gcctcgtcga tgacggtctt ctcttcggcg aacgcgcaca
     4081 gcatcgccgt gtcgcacagc acgttcacga gccgcggcac gccgcccgcg taacgaaaga
     4141 cgagatcgca agcggccggc tcgaagatcc ttcggccctt cgctcccgag acctcgagcc
     4201 ggtgcgtgat gtactcgtga gtttcgcgct tggagagcgc gcccaagtgg aaacgcagac
     4261 gaacgcgctg agtcaactgc tgcaggcgcg gcgagtcgag cttgtgcgag agctcgggct
     4321 ggcccgcgat gatgatgcgc agcagctttt ccttctgcag ctcgaggccc gacaggagcc
     4381 gaacttcctc gagcaccttg cgcgacagat tctgcgcttc gtcgatgacg agcaaaatct
     4441 tcttgccggc cgcgtactgc tcgaccatga agtccttgat catcgagagg agctcgacct
     4501 tgcgcttgcg gaacggcttg aagccgaact cgacgagcaa ggcttgcgtt caaagcaacc
     4561 gcgagttcag ataacggacg tattcgcgat gtgcggcggt ccagatgacg cgtgcgtcgt
     4621 cgagcgcttg aagcgtcgac gagcacgcgg gcatccctgc ctgacctccc agtaaagctg
     4681 acactgttcg tcagtgcggc cgttttggtt gcggcttgct gtcgtgcacc tcgccgcgcc
     4741 aagcgccgtc ctcgacgccg cggctctcga tgtacttctt gaactgcttc acgctcttct
     4801 cgacacgcgt gctcaacagg ccgagcgcgt cgccgacatt ctccgtcgcg ccttgcggct
     4861 cataggccat gacgaggcga accaacgttc gatcggggcc gatcggctcg aagcggacgg
     4921 tgccggcgtt cggcgcatcg cccgagacgc tcttccagcc gatcttctgg tc
//
LOCUS       DASSNN010000700         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_249372, whole genome shotgun sequence.
ACCESSION   DASSNN010000700 DASSNN010000000
VERSION     DASSNN010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249372"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VFL84_13145"
     CDS             complement(<1..839)
                     /locus_tag="VFL84_13145"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7609619.1"
                     /translation="MANGAEQWVTVATGLTEARANELGLVLTARNVPFQRQAGSQGWE
                     LWVPLADAPAAANELTLYRHENLKPVGGTRPVEEVAAGRAGVIWYLATLSTLFFALHT
                     FLFDLDWLAAGRLEAGPLLHGEWWRAVTALTVHVELGHLLGNLAFGAFYGYFVGRYLG
                     VGVGWLAVLLAASGANVLNAWVESPAHRSIGASTAVFAALGMLATYTWRRGYLRGTPW
                     RARIAPIVASIGLLAFTGTSGENTDLGAHLFGFIAGLATGLALARFASVTWLRTPRVQ
                     RIAG"
     gene            932..1435
                     /locus_tag="VFL84_13150"
     CDS             932..1435
                     /locus_tag="VFL84_13150"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019410888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET7609620.1"
                     /translation="MALHSARRAATAYCYWDLVTSPFRSLCVAVDGEGRLIEIRFDRR
                     RPEGRRDFKRCSDAARQLGEYFAGKRREFTLELAPVGTDFQLRVWRALGKIPYGAVRN
                     YADIARAIGQPGAARAVGQANGCNPLPIVIPCHRVIASDGTIGGYSGGLAIKHRLLAL
                     EGAELDL"
     gene            1439..1738
                     /locus_tag="VFL84_13155"
     CDS             1439..1738
                     /locus_tag="VFL84_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609621.1"
                     /translation="MANDLRCWRCGEPLAALTLPLARLDECPKCRVHLHVCRMCIHYA
                     PTKPKGCREDDAIEVRDKKAANFCDYFKPNAQAFDSADQQAEQKARNQLDDLFKK"
     gene            1828..>2122
                     /locus_tag="VFL84_13160"
     CDS             1828..>2122
                     /locus_tag="VFL84_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018916501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609622.1"
                     /translation="MFAKLKLLGAAAVLVGASAAAVAGDYGFDITNDTGDTITYLYVS
                     PETSNYWEEDVLGKDGLLRPGRTQHVRLSGYKTPIFDIRLVDQDGDRYTFWKVD"
BASE COUNT          372 a          750 c          702 g          298 t
ORIGIN      
        1 ccggcgatgc gctgcacgcg cggcgtgcgc agccatgtca ccgatgcgaa gcgcgcgagc
       61 gcgaggcccg ttgcgagccc cgcgatgaag ccgaacaggt gcgcgcccaa gtcggtgttc
      121 tcgccgctcg tgcccgtgaa cgcgagcagg ccgatgctgg cgacgatcgg cgcgatgcgt
      181 gcgcgccacg gcgtgccgcg caagtagccg cgccgccacg tgtacgtcgc gagcatgccg
      241 agcgcagcga acacggccgt cgacgcaccg atcgagcgat gcgccggcga ctcgacccac
      301 gcgttcagca cgttggcgcc ggaggccgcg agcagcaccg cgagccagcc gacgcctacg
      361 ccgagatacc ggcccacgaa gtagccgtag aacgcgccga acgcgaggtt gccgaggaga
      421 tggccgagct cgacgtgcac ggtcagcgcc gtgacggcgc gccaccattc gccgtgcaat
      481 aacggacccg cctcgagccg gccggcagcg agccagtcga gatcgaacag gaacgtgtgc
      541 aacgcgaaaa atagagtcga aagcgtcgcg aggtaccaaa tcacgccggc gcgccccgcg
      601 gcgacttcct cgaccggccg cgtgccgccg acgggcttca ggttctcatg ccggtacagt
      661 gtcagctcgt tcgccgcggc cggcgcgtcg gcgagcggga cccagagctc ccagccttgc
      721 gagccggcct gccgctggaa cggcacgttg cgcgcggtca agacgagccc gagctcgttc
      781 gcgcgcgcct cggtgaggcc ggtcgcgacc gttacccact gctccgcgcc gttcgccatc
      841 tgatagcatc ccacgccata acaactaagc cgcttaggct tcggcgccac ggcggcacgc
      901 taaggtagca aacttggggc aaccggcagc catggccctt cactccgcgc ggcgcgccgc
      961 gacggcgtat tgctactggg atctcgtgac ctcgccgttc cgctcgctct gcgtcgccgt
     1021 cgacggcgag ggccggctca tcgaaattcg cttcgaccgc cgccgccccg aagggcgccg
     1081 cgacttcaag cgctgcagcg acgccgcgcg gcagctcggc gagtacttcg ccggcaagcg
     1141 gcgcgagttc acgctcgagc tcgcgcccgt cggcacggat ttccagctgc gcgtctggcg
     1201 cgcgctcggc aagatcccgt acggcgccgt gcgcaattac gccgacatcg cgcgcgcgat
     1261 cggccaaccc ggcgccgcgc gcgccgtcgg ccaggcgaac ggctgcaacc ctctgccgat
     1321 cgtgatcccg tgccaccgcg tcatcgcgag cgacggcacg atcggcggct attccggcgg
     1381 cctcgcgatc aagcatcgct tgctcgcgct cgaaggcgcc gagctcgatc tctagacgat
     1441 ggcgaacgat cttcgctgct ggcgctgcgg cgagccgctg gccgcgctca cgctgccgct
     1501 cgcgcggctc gacgagtgcc cgaagtgccg cgtgcatctg cacgtctgcc gcatgtgcat
     1561 ccattacgcg ccgacgaagc cgaaaggctg ccgcgaggac gacgccatcg aggtccgcga
     1621 caagaaggcc gcgaacttct gcgactactt caagccgaac gcgcaggcat tcgactccgc
     1681 cgaccagcag gccgagcaaa aggcgcgtaa ccagctcgac gatctgttca agaaatagcg
     1741 gaaccgtagc caaacggcga cacgctgcca ggtgccaacc ggctgagtag tatccggccg
     1801 gctcaaaagg accttggagg tttcgccttg tttgccaagc tcaagctgct cggtgcggcc
     1861 gccgtgctcg tgggcgcgag cgccgccgcc gtcgccggcg actacggttt cgacatcacc
     1921 aacgacaccg gcgacacgat cacgtatctg tacgtgagcc ccgagacatc gaactactgg
     1981 gaagaggacg tgctcggcaa ggacggcctg ttgcgcccag ggaggacgca gcacgtgagg
     2041 ttgtcgggct acaagacgcc gatcttcgac attcgcttgg tcgaccagga cggcgaccgg
     2101 tatacgtttt ggaaagtcga cg
//
LOCUS       DASSNN010000701         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_354330, whole genome shotgun sequence.
ACCESSION   DASSNN010000701 DASSNN010000000
VERSION     DASSNN010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354330"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..2050
                     /gene="ftsH"
                     /locus_tag="VFL84_13165"
     CDS             146..2050
                     /gene="ftsH"
                     /locus_tag="VFL84_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET7609623.1"
                     /translation="MNNLLKNIGIWLVIGLVVLTVVKQFDSRQSAKDSVAYSDFLESA
                     RSGRVESAVVEGRSIKWVSTDKKRYVTYSPGDIWMVGDLVKYGVKVEARPEEEQSFLA
                     QIFISWFPMLLLIGVWIFFMRQMQGGGRGGAFSFGKSKARMLDESNNTVTFADVAGCD
                     EAKEEVAELVEFLRDPTKFQKLGGRIPRGVLMVGSPGTGKTLLAKAIAGEAKVPFFSI
                     SGSDFVEMFVGVGAARVRDMFEQAKKNAPCIVFIDEIDAVGRQRGAGLGGGNDEREQT
                     LNQLLVEMDGFEGSAGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQILL
                     VHMRKVPMAPDVKPDIIARGTPGFSGADLANLVNEAALFAARGNKRLVDMLDFERAKD
                     KIIMGAERRSMVMPEEERRNTAYHESGHAIVAMLLPKTDPVHKVTIIPRGRALGVTMQ
                     LPETDRYSLDKDRMLNTVAVLFGGRIAEELFMNQMTTGASNDFERATQIARDMVMRYG
                     MSDLMGPMVYGDNEGEIFLGRAITRTVNMSEETMRNVDAEIRGIIDQQYALARKLIEE
                     NRDKVEAMAKALLELETIDSDQIGDIMSGKPPRPPKPVPTPAPGTTIGPGTVGGTAPS
                     TNPAKSGLDG"
BASE COUNT          432 a          728 c          799 g          370 t
ORIGIN      
        1 tttgtaacgc tttgcacccc cggcgcagcc gtggagcgcg attacacgta gaatgggaat
       61 cggcgcagtc cgctcgcggg ccgcgcgttc atttatcacc cggcgggccg gccggaacga
      121 cgctagcggc cgcgaggagc aacgcttgaa caatttgttg aagaacatcg gaatctggct
      181 cgtcatcggg ctcgtcgtgc tgaccgtcgt caagcaattc gactcccgcc agtcggccaa
      241 ggattccgtc gcgtattccg atttcctgga gtcggcgcgc agtggccgcg tcgaatccgc
      301 ggtcgtcgaa ggccgctcga tcaagtgggt atccaccgac aagaagcgct acgtcacgta
      361 cagccccggc gacatctgga tggtgggcga cctcgtcaag tacggcgtca aggtcgaggc
      421 gcgtccggag gaagagcaga gcttcctcgc gcagatcttc atctcgtggt tcccgatgct
      481 gttgctgatc ggcgtgtgga tcttcttcat gcgacagatg cagggcggcg gcaggggagg
      541 tgcgttcagc ttcggcaaga gcaaggcgcg catgctcgac gagtcgaaca acaccgtgac
      601 gtttgccgac gtcgccggct gcgacgaggc gaaggaggag gtggccgagc tcgtcgaatt
      661 cctgcgtgac ccgacgaagt tccagaagct cggcggacgc attccgcgcg gcgtgctgat
      721 ggtcggctcg ccgggcaccg gcaagacgct gctcgcgaag gcgatcgccg gtgaggcgaa
      781 ggttccgttc ttctcgatct ccggctccga cttcgtcgag atgttcgtgg gcgtcggcgc
      841 tgcgcgtgtg cgcgacatgt tcgagcaggc gaagaaaaac gcgccttgca tcgtgttcat
      901 cgacgaaatc gacgccgtcg gccgccagcg tggcgcggga ctcggcggcg gcaacgacga
      961 gcgcgagcag acgctcaatc aattgctggt cgagatggac ggcttcgaag gcagcgcagg
     1021 cgtgatcgtc atcgccgcta cgaaccggcc tgacgtcctc gatcccgcgt tgctgcgtcc
     1081 gggccgcttc gatcgccagg tggtcgtgcc gctgcccgac atccgcggcc gcgagcagat
     1141 cctgctcgtg cacatgcgca aggtgccgat ggcgccggac gtcaagcccg acatcatcgc
     1201 ccgcggcacg cccgggttct cgggcgcgga tctcgcgaac ctcgtcaacg aggcggcgct
     1261 gttcgccgcg cgcggcaaca agcggctcgt cgacatgctc gacttcgagc gcgcgaagga
     1321 caagatcatc atgggcgccg agcggcgctc gatggtgatg cccgaggagg agcgccggaa
     1381 caccgcgtat cacgagtcgg gtcacgcgat cgtcgcgatg ctgctgccga agaccgatcc
     1441 cgtgcacaag gtgaccatca tcccgcgcgg gcgtgcgctc ggcgtgacga tgcagctgcc
     1501 cgagaccgat cgctacagcc tggacaagga ccgcatgctg aacacggtcg cggtgctctt
     1561 cggcgggcgc atcgccgaag agctgttcat gaaccagatg accaccggcg cctcgaacga
     1621 cttcgagcgc gcgacgcaga tcgcgcgcga catggtgatg cgctacggga tgagcgacct
     1681 gatggggccg atggtgtacg gcgacaacga gggcgagatc ttcctcggcc gtgcgatcac
     1741 gcgcaccgtc aacatgtcgg aagagacgat gcgcaacgtc gacgctgaga ttcgcggcat
     1801 catcgaccag cagtacgcgc tcgcgcgcaa gctgatcgag gagaaccgcg acaaggtcga
     1861 ggcgatggcg aaggcgctgc tcgagctcga gacgatcgac tccgatcaaa tcggcgacat
     1921 catgtccggc aagccgccgc ggccgcccaa gcccgtgccg acgcccgcgc cgggcacgac
     1981 gatcggtccc ggaacggtgg ggggcacggc accatcgacg aatccggcca aatcggggct
     2041 cgatggctga ccaatcgagc agcgcgacga agaggtgcac cggcgtgcgc ctcttttttt
     2101 gcgctttgca gattgacggg aagcatggcg taggacgcgg gcaatgcgtg tccggcgctc
     2161 caggtcgttg acgtcggccg aggcaaggcc cgagcaggcc cgaagggcgc gacgccgcgc
     2221 caaggagaac ggccgacgcg ctgccagatg catatcaaca agtgcaagga ggcgcgggct
     2281 ttcacggact cggagcgtgc gccgcacgtt gacggctgcc aatgcacat
//
LOCUS       DASSNN010000702         5592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1868022, whole genome shotgun sequence.
ACCESSION   DASSNN010000702 DASSNN010000000
VERSION     DASSNN010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868022"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1019
                     /locus_tag="VFL84_13170"
     CDS             39..1019
                     /locus_tag="VFL84_13170"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7609624.1"
                     /translation="MIQRFCASLVACAVVTTAAAQAPADAPAADSARVAPFVARARAA
                     AGNEWGATVDFFCGDAARANRADDPEMKPTRVFDNLYALGRTSTVVWALTTPDGILLI
                     DAGYPDQLDSVLLPQMKAAGLDANDVHYVLVAHGHSDHFGAASYFQQRGARVALSDAD
                     WSLIENPPPRPAGAPPAPPAAGLPPPRRDVVVADGTPISFGGVEITPVLIPGHTPGAL
                     GFVFRVMDGAQAHTAALFGGSILTSNRISDDGLRQYVQSLGRFAAVTQRLGVDVEIQN
                     HPLYDGFPQKLERLAARRAGEPHPFVVGTGAYQRFLTVMSECANAQLARR"
     gene            complement(1016..1948)
                     /locus_tag="VFL84_13175"
     CDS             complement(1016..1948)
                     /locus_tag="VFL84_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609625.1"
                     /translation="MRSFNSLLIAAAVVFVGAASAQPPPGGPPRGGPGYPQVYDPKMP
                     FDAKEIAGIWTPNAVGFGGGGRCRDCGDRGYSNEFPVFTEEGQAAFDANTPSYGRAAG
                     SPDAAAHPEEHIGRRRAQPPALGNDLYQSCNPMGIPRAILYPDPEEFIVLPDRILQHF
                     QWGYGLRTIWMDGRTLPKPEEIDIARWWGYAVGRWEGNTLVVTSTGYDGRTWVDHFGY
                     PHSDMMVLEERYTRINYDTLELKMTLTDPVYYQKPWVSETKRMHLIPRDFIKSSDWSG
                     LLEDLCAPVDEVDTFNRLIRDPAGTGQPAAPPSR"
     gene            complement(1959..2399)
                     /locus_tag="VFL84_13180"
     CDS             complement(1959..2399)
                     /locus_tag="VFL84_13180"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609626.1"
                     /translation="MKRAALSCTLIGALFAAVALPAFAHHGTLVSYDRDKQWTREVTV
                     TRFNYANPHPQIFFDVTDEKGTVVHWAGELLPNPAGLLRVGWTKSRSLQALAPGTKIN
                     ITIAPARAGGLVGLVVRIKNLDGADIVSDSVPYPDTAAAPAPQP"
     gene            complement(2442..3344)
                     /locus_tag="VFL84_13185"
     CDS             complement(2442..3344)
                     /locus_tag="VFL84_13185"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7609627.1"
                     /translation="MNLRQFDLNLLVALDVLLTERNVTRAGERLFLSQPAVSGILARL
                     RHAFQDELLVRVGRNLELTAFAADLAGPVHDAVRQIEDLLNLRRPFEPESAQWSFRVA
                     ASDYVVFLLLGTLLRSLAECAPNVSVRILALEPTAGERLAAGELDFAVLPAEIEPSLP
                     SLPLFEDTWVCAVWSGHPHVGEHFTLDEFLAIPHLSFRLAGPDHGSIAEGHLAELGFE
                     LKIVASTESFATAPFLLRDTPFATLVPRRLGERLRQAADIRLVELPLDLPPLREKLIW
                     SPRYTASPAHVWFRTRIAEIAQAL"
     gene            complement(3341..4270)
                     /locus_tag="VFL84_13190"
     CDS             complement(3341..4270)
                     /locus_tag="VFL84_13190"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET7609628.1"
                     /translation="MTTPLRLSTAALIFALAVCAPLQSQTGNASRGALKPGRTFSECR
                     NCPEMMVLPAGKVSIGSPPDEPLRRDNEPQKEITFARAFAISRTAVTWDQWEACVRDR
                     WCDGAAIDAALRTGQNGEPNPDYRDYGRGTRPVVGVSWYDAQGFVGWLNWKTDEDDAY
                     RLPSEAEWEYAARGGTTTAFPWGAELDHDYGNFGKEAGGLGGKAEGRDTWIDETAPVA
                     SFPPNAFGLYDMHGNVFEWTEDCYEADRAHTPADGSANKEGNCANRVFRNGTFMSNPY
                     MQRSARRGAPYPATQRGRNYLGFRVAKTLPGAQ"
     gene            complement(4273..5316)
                     /locus_tag="VFL84_13195"
     CDS             complement(4273..5316)
                     /locus_tag="VFL84_13195"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 family protein"
                     /protein_id="HET7609629.1"
                     /translation="MNKTNPIPLLCAVLLLVFAPLAAQPQTSQRTLIPHATWNCGMPE
                     GIPRPEAGRLVFEIEVPLERAIDVGHTQYGMRRVAVGLDGAVKGPKFSGVVAPGTLDF
                     ELTLANGTVEIEQTLVLKAADGSYVYARNAGTGPSADDIRVVMDFEAPNAGEHAWLNS
                     GQFVARRELNVAAKMLRLRVYEVAGVPAGSGKDGTLTIAKPSNVPAQPWNYRLKEAGE
                     QPGELLITENVTLAPSQRVGASKRGDRNIIPITGGTVKGRISGKVLMGGADFQLLTPP
                     ATIDAHYLWETDDGEVIVVRNGGAFGALVPTFEARADGPYAYLNEGAYLSSNPGRGEG
                     GVALTFHESARAR"
     gene            complement(5334..>5592)
                     /locus_tag="VFL84_13200"
     CDS             complement(5334..>5592)
                     /locus_tag="VFL84_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609630.1"
                     /translation="ERTFFWRAGTGAAKQTAVRRGDWKLVIDGPHTYVFNLRTDLSER
                     NDLARWRQDIAQQLYPLIASWEASVDAEAKTLGSTNGGSGL"
BASE COUNT          881 a         1940 c         1846 g          925 t
ORIGIN      
        1 cactaaacta tggtctcgtg gagccgggag gggaagccat gatccaacga ttctgtgcgt
       61 ctctggttgc gtgtgccgtc gtcaccaccg ccgcggcgca ggcgccggcg gacgcacccg
      121 cggccgactc cgcgcgcgtc gcgcccttcg tcgctcgcgc ccgcgcggcc gccggcaacg
      181 aatggggcgc aaccgtggac ttcttctgcg gcgatgcggc ccgcgccaat cgcgccgacg
      241 accccgagat gaagccgacg cgcgtgttcg acaacttgta cgcgctcggc cgcacgagca
      301 cggtcgtctg ggcgctgacg actccggacg gcatcttgtt gatcgacgcc ggctaccccg
      361 atcagctgga ctcggtgctg ctgccgcaga tgaaagcagc cgggctcgac gcgaacgacg
      421 tgcactatgt gctcgtcgcg cacggccatt ccgatcactt cggcgccgcg tcgtactttc
      481 agcaacgcgg cgcgcgtgtc gcgctgtccg acgccgactg gagcttgatc gagaacccgc
      541 cgccgcgccc agccggcgcg ccgcccgccc cgcccgcagc cggattgccg ccgccgcgcc
      601 gcgacgtcgt cgtggccgac ggcacgccga tctcgttcgg cggcgtcgag atcactcccg
      661 tgctgatccc cggacacacg cccggcgcgc tcggcttcgt gttccgcgtc atggacggcg
      721 cgcaggctca caccgccgcg ttgttcggcg gctcgatatt gacgagcaat cgcatctccg
      781 acgacggctt gcggcaatac gtgcaatcgc tcgggcgttt cgccgccgtg acgcagcggc
      841 tcggcgtcga cgtcgagatc cagaatcatc cgctctacga cggattcccg cagaagctcg
      901 aacggctggc ggcgcgccgc gccggcgagc cgcatccgtt cgtcgtcggc accggcgcgt
      961 atcaacgctt cctgaccgtc atgagcgagt gcgcgaacgc gcagctcgcg cgccgttaac
     1021 gcgacggcgg agccgccggc tggcccgtgc ccgccgggtc gcgaattagc cggttgaacg
     1081 tgtcgacctc gtcgacgggc gcgcacagat cctcgagcaa gccgctccaa tcggacgact
     1141 tgatgaagtc gcgcgggatg aggtgcatgc gcttcgtctc cgagacccac ggcttctggt
     1201 agtagacggg atcggtcaac gtcatcttga gctcgagcgt gtcgtagttg atgcgcgtgt
     1261 agcgctcttc gagcaccatc atgtcgctgt gcggatagcc gaagtgatcg acccacgttc
     1321 ggccgtcgta tccggtcgac gtgacgacga gtgtgttgcc ttcccagcgt ccgaccgcgt
     1381 agccccacca gcgcgcgatg tcgatttctt ccggcttcgg caacgtgcgg ccgtccatcc
     1441 agatcgtgcg caagccgtag ccccactgga agtgctgcag gatccgatcc ggcaacacga
     1501 tgaattcctc tggatcggga tagaggatcg cgcgcgggat gcccatcgga ttgcagctct
     1561 ggtagagatc gttgccgagc gctgggggct gtgcgcggcg ccggccgatg tgctcttcgg
     1621 gatgcgcggc ggcgtcgggg ctgccggctg cgcggccgta cgacggcgtg ttcgcgtcga
     1681 acgcggcctg cccttcctcg gtgaacacgg gaaactcgtt gctgtagccg cggtcgccgc
     1741 agtcgcgaca gcgccctccg ccgccgaagc cgacggcgtt cggcgtccag atgcccgcga
     1801 tctctttcgc atcgaacggc atcttcgggt cgtagacctg cgggtaaccc ggaccgcctc
     1861 gtggcgggcc gcccggcggc ggctgagccg aagcggcgcc gacgaacacg accgcggccg
     1921 cgatcagaag cgaattgaac gatcgcatag cggcgcgctc agggctgtgg cgcgggcgcc
     1981 gcagcagtat cgggatacgg caccgagtcg gacacgatgt ccgcaccgtc gaggttcttg
     2041 attctgacca ccaagccgac gaggccgccg gcgcgtgccg gcgcaatcgt gatgttgatc
     2101 ttcgtgccgg gcgcgagcgc ttgcagcgag cgcgacttcg tccagccgac gcgcagcaag
     2161 ccggccgggt tcggcaacag ctcgccggcc caatgcacga ccgtgccctt ctcgtcggtg
     2221 acgtcgaaga agatctgcgg atgcggattc gcgtagttga agcgcgtgac cgtcacttcg
     2281 cgcgtccact gtttgtcacg atcgtaggaa acgagcgtgc cgtgatgcgc gaatgcgggc
     2341 agcgccaccg ccgcgaacaa tgcgccgatg agagtgcaag acaacgctgc gcgtttcacg
     2401 acgccccccg gcaagagatg tccccgtccg ttctcattct ctcacagggc ctgcgcgatc
     2461 tcggcgatcc gcgtccggaa ccagacgtgc gcggggctcg ccgtgtagcg cgggctccaa
     2521 atcagcttct cgcggagtgg cgggaggtcg agcggcagct cgacgagcct tatgtcggcg
     2581 gcctgccgca gccgctcgcc gagccggcgc ggcacgagcg tcgcgaacgg cgtgtcgcgc
     2641 agcaagaacg gcgccgtcgc aaagctctcg gtcgaggcga cgatcttgag ctcgaacccg
     2701 agctccgcga gatggccctc cgcgatcgag ccgtgatcgg ggcccgccag gcggaagctc
     2761 agatgcggga tcgcgaggaa ctcgtcgagc gtgaagtgct cgcccacgtg cgggtgcccc
     2821 gaccagacgg cgcagaccca ggtgtcctcg aagagcggca gcgacggcag gctcggctcg
     2881 atctccgcgg gcaagaccgc aaagtcgagc tcgccggcgg cgagccgctc gccggcagtc
     2941 ggctcgagcg caaggatccg caccgagacg ttcggtgcac actcggccag cgagcgcagc
     3001 aacgtgccga gcagcaggaa cacgacgtaa tcgctggccg cgacgcgaaa cgaccactgc
     3061 gcgctctcgg gctcgaacgg gcgccgcaag ttcaacaggt cttcgatctg gcggaccgcg
     3121 tcgtgcaccg gccccgcgag atccgcggcg aacgccgtca gctcgaggtt ccggcccacg
     3181 cgcacgagca gctcgtcctg aaatgcatgc cgcaaccgcg cgaggattcc gctgaccgcg
     3241 ggctggctca gaaacaagcg ctcgccggcg cgcgtgacgt tgcgctccgt gagcagcacg
     3301 tcgagcgcga cgagcaggtt cagatcgaat tgccgaaggt tcattgagct ccgggcaacg
     3361 tcttggcgac gcggaacccg agataattgc ggccgcgctg cgtggccgga tacggcgcgc
     3421 cgcgccgcgc cgagcgctgc atgtacgggt tgctcatgaa cgtgccgttg cggaacacac
     3481 ggttcgcgca attgccttcc ttgttggccg agccgtcggc cggcgtgtgc gcgcggtcgg
     3541 cttcgtagca gtcttcggtc cactcgaaga cgttgccgtg catgtcgtag aggccgaacg
     3601 cgttcggcgg gaacgatgcg acgggcgccg tctcgtcgat ccaagtatcg cgtccttccg
     3661 ctttgccgcc gagaccaccg gcctccttgc caaaattgcc gtaatcgtga tcgagctcgg
     3721 cgccccacgg gaacgccgtc gtcgtgccgc cgcgcgcggc gtactcccac tccgcttccg
     3781 acggcagccg atacgcgtcg tcctcatcgg tcttccagtt cagccaaccg acgaaccctt
     3841 gcgcgtcgta ccagctcacg ccgacgacgg gccgcgtgcc gcggccataa tcacggtaat
     3901 cgggattggg ctcgccgttc tgcccggtgc gcagcgccgc gtcgattgcg gcgccgtcgc
     3961 accaacggtc gcgcacgcac gcctcccact ggtcccacgt cacggccgtg cgcgagatcg
     4021 cgaacgcgcg tgcaaacgtg atttccttct gcggctcgtt gtcgcgacgc agcggctcgt
     4081 cgggcggcga gccgatcgac acctttccgg ccggcaacac catcatctcc gggcagttgc
     4141 gacattcgct gaaggtccgg cccggtttca gcgcgccgcg cgacgcgttg ccggtctggc
     4201 tctgcagcgg cgcgcatacg gcaagcgcga agatcagcgc tgccgtcgac aaacgaagtg
     4261 gtgtcgtcat ggctaacgag ccctcgcgct ctcgtggaac gtcaacgcga cgccgccctc
     4321 gccccgcccc gggttcgagc tcaagtacgc accttcgttc agatacgcgt acggaccgtc
     4381 ggcgcgcgcc tcgaacgtcg gcacgagcgc gccgaacgcg ccgccgttgc gcacgacgat
     4441 gacctcgccg tcgtccgtct cccacaaata atgcgcgtcg atcgtcgcgg gcggcgtaag
     4501 gagttggaag tcggcgccac ccatcagcac cttgcccgag atgcggccct tcacggtgcc
     4561 gcccgtgatc ggaatgatgt tgcgatcgcc gcgcttgctc gcgccgacgc gctgcgacgg
     4621 agcgagcgtc acgttctcgg tgatcagcag ctcgcccggt tgctcgcccg cctccttgag
     4681 gcggtagttc cacggctgcg ccggaacgtt cgacggcttc gcgatcgtga gcgtgccgtc
     4741 cttaccggag ccggccggca cgcccgctac ctcgtataca cgcagccgca gcatcttcgc
     4801 cgcaacattc agctcgcgcc gcgcaacgaa ctggcccgag ttgagccagg cgtgctcgcc
     4861 ggcgttcggc gcctcgaagt ccatgacgac gcggatgtcg tcggcgctcg gccccgtgcc
     4921 ggcgttgcgc gcatagacgt agcttccgtc ggcggctttc aacacgagcg tttgctcgat
     4981 ctcgacggtt ccgttcgcga gcgtcagctc gaaatcgagc gtgccgggcg cgaccacgcc
     5041 ggaaaacttc ggccctttga cggcgccgtc gagcccgacg gcgacgcgcc gcattccgta
     5101 ctgcgtatgt ccgacgtcga tcgcgcgctc gagcgggacc tcgatctcga ataccaagcg
     5161 gccggcttcg ggacgcggga tcccttccgg catgccgcaa ttccaggtcg cgtggggaat
     5221 caacgtgcgc tgcgaagtct gcggttgcgc ggccagaggc gcgaacacga gcagcaacac
     5281 tgcgcacagc aacggaatcg ggttggtctt gttcatatcc tcgagtcccc gcgttacaaa
     5341 ccgctgcctc cgttcgtcga accgagcgtc ttcgcttcgg cgtctactga agcttcccag
     5401 ctcgcgatca acggatagag ctgctgcgcg atgtcctgac gccagcgcgc aagatcgttg
     5461 cgctcgctca ggtccgtgcg caggttgaac acgtacgtat gtggcccgtc gatgacgagc
     5521 ttccaatctc cgcgcctgac ggcagtctgc tttgcagcgc ccgtgccggc tcgccagaag
     5581 aacgttcgct cg
//
LOCUS       DASSNN010000703         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_859695, whole genome shotgun sequence.
ACCESSION   DASSNN010000703 DASSNN010000000
VERSION     DASSNN010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859695"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VFL84_13205"
     CDS             <1..772
                     /locus_tag="VFL84_13205"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7609631.1"
                     /translation="DNRRLEDFEVLIEGKVERRIVFAPDGTMYVTGADAFRFYDSDLD
                     SIEGRVFTDNPDVRRNFTGRVIRINPDGTVPVDNPWLGRATVARETFAHGFKDPEGAA
                     INPATGELWLVDHGPQGGDEINIVRGGHDYGWPEVSYGVQYDARQADGRKNVAVGSGK
                     TSAPGVDEPIYYWVPSIAPSGMAFYTGTLFPEWRGNLFVGAMAGQHLVRLVLDGERVI
                     AEEKLLEELGVRIREVRQGPDGALYVFGGDSLIRITPP"
     gene            complement(795..2174)
                     /locus_tag="VFL84_13210"
     CDS             complement(795..2174)
                     /locus_tag="VFL84_13210"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET7609632.1"
                     /translation="MMRFQGIVAGFAALLATATTVAQPPGPPPPAPLDGAARTAVVKA
                     AADALRQRYVYPEVGKRAAEAIEAALAAGTYDEIVQPWAFAERLTADLREVAHDKHMN
                     ITARGPAPVPAAATGLPPPGPPPRSEAGVTRSDRLPGNIGYIEVVALPALDAFKGPVD
                     KAMAALADTRALIVDVRRNGGGAPEAEVYLASYFLDPAKPIVVNTFVSRVPNTETFRT
                     REFTSSPTPYWYRGKPVYVLTSAATFSGGEGVAYDMQVHKLATLVGETTGGGANPGGG
                     MPLTPDFGMFMPGGRAESPITGTNWEGVGVKPDVAVPAADALKTALGLLGQKTDKTEI
                     AALSEARFFEPRNTANPLSEPAVRRMIAELVRGEPNYDLLSPGMQEVTRAQLPGLQRM
                     FQSLGEIESVTFVEVGPQGGDVFDVKFANGAVRWNIALTADGKTAGAGVRPLGPLPGG
                     PPAAPPPQQ"
     gene            2325..2600
                     /locus_tag="VFL84_13215"
     CDS             2325..2600
                     /locus_tag="VFL84_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HET7609633.1"
                     /translation="MARKYSKKASKKVERAMHERKRGKLRSGRSGKKVTSRKQAIAIG
                     LSEARRSGAKVPKRRRRKTSSSRRKGSTRRRRTTSRRSSRRSSKRRA"
     gene            2794..>3082
                     /locus_tag="VFL84_13220"
     CDS             2794..>3082
                     /locus_tag="VFL84_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609634.1"
                     /translation="MIELLTAVEDSGLSVFIREWPSVFGFPTILFLHTLGLAMVAGTS
                     IAIDLWILRGANAGAPLHLLGLTRTMWLGFGINLLSGLALLLAYPAKALTNS"
BASE COUNT          519 a         1019 c         1038 g          506 t
ORIGIN      
        1 cgacaatcgc cgcctcgagg acttcgaggt gctgatcgaa ggcaaggtgg agcggcgcat
       61 cgtgttcgcg ccggacggca cgatgtacgt gaccggcgcc gacgcgttcc gcttctacga
      121 ctccgacctc gacagcatcg aaggccgcgt gttcacggac aaccccgacg tccgccgcaa
      181 cttcacgggc cgcgtcattc gcatcaaccc cgacggcact gtacctgtgg acaatccgtg
      241 gctcggacgc gcgacggtgg cgcgcgagac gttcgcgcac ggtttcaagg atcccgaggg
      301 cgccgcgatc aacccggcga ccggcgagct ctggctcgtc gatcacggcc cgcaaggcgg
      361 cgacgagatc aacatcgtgc gcggcggcca cgactacggc tggcccgagg tttcgtacgg
      421 cgtgcaatac gacgcgcgcc aagcggacgg ccgcaagaac gtcgcggtcg gcagcggcaa
      481 gacgtcggcg ccgggcgtcg acgagccgat ctattactgg gtaccgtcga tcgcgccttc
      541 gggcatggcg ttctatacgg gcactctctt tcccgagtgg cgcggcaatc tcttcgtcgg
      601 cgcgatggcg ggccagcacc tcgttaggct cgtgctcgac ggcgagcgcg tgatcgccga
      661 ggaaaaacta ctggaagagc tcggcgtccg catccgcgag gtgcggcaag gccctgacgg
      721 cgcgctctac gtgttcggcg gcgactcgct gatccgcatc acgccgccgt agcgataccc
      781 gctgcccacg cgctctattg ctgcggcggc ggggctgccg gcggccctcc cggcagcggg
      841 cccagcggac gaacgcccgc tccggccgtc ttgccatccg cggtcagagc gatgttccaa
      901 cgcaccgcac cgttcgcgaa cttgacgtcg aaaacgtcgc ccccttgtgg gccgacttcg
      961 acgaaggtca cggactcgat ttcgcccaac gactgaaaca tgcgctggag tccaggcagc
     1021 tgcgcgcgcg tcacttcctg cattccgggc gacagcaagt cgtaattcgg ctcgccacgc
     1081 acgagctcgg cgatcatgcg gcgaacggcg ggctccgaaa gcgggttggc ggtattgcgc
     1141 ggctcgaaga aacgcgcctc cgacaacgcg gcgatctcgg tcttgtcggt cttttgcccg
     1201 agcagaccga gcgcggtctt gagtgcgtcg gcggcaggca ccgcgacgtc gggcttcacg
     1261 ccgacgccct cccagttcgt gccagtgatc gggctctcgg cgcggccgcc cggcatgaac
     1321 attccaaagt ccggcgtcag cggcatacct ccgccggggt ttgcgccgcc gcccgtcgtc
     1381 tcgccgacga gcgtcgcgag cttgtggact tgcatgtcgt aggcgacgcc ctcgcccccg
     1441 gagaaggttg cagcgctcgt cagcacgtag acgggcttgc cgcgatacca gtacggcgtc
     1501 ggcgagctcg tgaactctct ggtgcgaaac gtctcggtgt tcgggacgcg cgacacgaac
     1561 gtgttgacga cgatcggctt cgcggggtcg agaaagtaac tcgccagata gacctccgcc
     1621 tccggcgcgc cgccgccgtt gcggcgcacg tcgacgatca atgcgcgcgt gtcggcgagg
     1681 gcagccatgg ctttgtcgac cgggcccttg aacgcgtcga gcgcgggtaa tgcgacgacc
     1741 tcgatgtagc cgatgttgcc gggcaaccgg tcggagcgag tgacgcccgc ctcgctgcgc
     1801 ggcggaggac cgggcggtgg cagtcccgtt gctgccgcgg ggaccggcgc cggtccgcgt
     1861 gcggtgatgt tcatgtgctt gtcgtgcgcg acctctcgca aatccgccgt gagccgttcc
     1921 gcgaatgccc acggctgcac gatctcgtcg tacgttcccg ccgcgagcgc cgcttcgatc
     1981 gcttcggccg ctcgcttccc gacctcggga tagacgtacc gctggcgaag tgcgtccgcg
     2041 gccgccttga cgacggccgt gcgcgccgcg ccatcgagcg gcgcgggcgg tggcgggccg
     2101 ggaggttgag cgaccgtagt cgccgttgcg agaagagctg cgaaaccggc gacgatccct
     2161 tgaaaccgca tcatgaggat ctcctcggag cgctcggatc ggaggttaga tgcacgatca
     2221 tgttcgcgcg tcgtacacga tgcaatgggc gcaacggtcg cgtcccattt ttggcacgga
     2281 tcatgctgtt tcctgccacg acttcggtca cgacggagga aagcatggcc aggaagtaca
     2341 gcaagaaggc atcgaagaag gtcgagcgcg cgatgcacga gcgcaagcgc ggcaagctgc
     2401 gcagcgggcg ctccggcaag aaagttacga gccgcaagca ggcgatcgcg attggcctct
     2461 ccgaggcgcg ccgatcgggc gcgaaagtcc cgaagcgacg ccgcaggaag acgagcagca
     2521 gccgtaggaa gggcagcaca cgccgacgac gtaccacgag ccggcgcagc agcagacgca
     2581 gcagcaagcg ccgcgcgtaa gccggcggcg gccatccagc cggcgaccgc caatgccaaa
     2641 atggggactg ccaaaatggg gacggattta tttttgcgcc gtagactgac gagacaccgc
     2701 aagtcgatgt gggcgcataa atagatccgt ccccattttg ggagatgtaa atagatccgt
     2761 cccctttttg ggggtctggt acgctgcgcc gcgatgatcg agctactgac ggccgtcgag
     2821 gacagcgggc tcagcgtctt catccgcgag tggccgtcgg ttttcggctt tccgacgatc
     2881 ctgttcctgc acacgctcgg cctcgcgatg gtcgccggca cgagcatcgc gatcgatctg
     2941 tggatcctgc gcggcgccaa cgccggcgcg ccgctgcacc tgctcggcct gacccgaacg
     3001 atgtggctcg ggttcggcat caatcttctt tccggtcttg cgctgctgct cgcgtatccc
     3061 gcgaaagcgc tgacgaactc cg
//
LOCUS       DASSNN010000704         2579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1132925, whole genome shotgun sequence.
ACCESSION   DASSNN010000704 DASSNN010000000
VERSION     DASSNN010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132925"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..325
                     /locus_tag="VFL84_13225"
     CDS             47..325
                     /locus_tag="VFL84_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609635.1"
                     /translation="MRWLRLAAPALLLAAWWTGSFAQQQGTPPATTPPPTPPPASTPQ
                     PSTQRPAQPEQEKEDEKPQAPSDDAADEEFTPTEELQPDAAVTFPVDI"
     gene            337..1713
                     /locus_tag="VFL84_13230"
     CDS             337..1713
                     /locus_tag="VFL84_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7609636.1"
                     /translation="MGAIAIEINDAGLAVATESDVLAVEPGFARIEGGKIVTGEQARA
                     RARRQPRQTSSRFWSALSLEPGSAGADIGKSAAELAFAQLESVWQRVGSDATAVILVV
                     PGGYRTEQLGLLLGLAQECGMPVRALVDAAAAASVRPYPKRQLVYLDASLYRVSLSLL
                     EQNGEAQVRAEHALAQGLAAVTDAFARRIADHFVRATRLDPFTHAETEQALYDALPEW
                     LEALQREERFELALKYRNEEFRVTAERDAVLGVAKGPYRAFIQLVAQHREPGKRLVLQ
                     LSDRIAALPGLVGELSRLDDSQIERYAPGHAARSVLLVPSIAESQGDVKLLKRVPWRE
                     APSEDASDAARSAAEPRPMLARATPPTHVVYGGLAYRVGAAGLAIGREADPQRRTIVL
                     TGSNGVSRLHCEVVLRDGELKLRDLSSYGTFVNERKVAGETTLSRADVIRIGSPGAEL
                     HVVDVEDA"
     gene            1713..>2579
                     /locus_tag="VFL84_13235"
     CDS             1713..>2579
                     /locus_tag="VFL84_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609637.1"
                     /translation="MAKRRKGELNVFSMSFLDAITAGFGCVVLLFMLVSANAVLDQRT
                     AVENREAEARRWELRVLTGQRNLVQLREALEVQLREWTNLLAVRQGLVTQIQDTEAEL
                     ATLTEDSEARRAAIERLRKELAELNKQSQELVTNQREESDDATRLRAFQGEGNRQYLT
                     GLRMGGKHVVILVDTSTSMLDRTIVNILRRRHMTPEQQRRSPKWRQVVNTVDWLSTQI
                     APGTDVQIIGFTDKATWLIPGTEGKWVTINDGSELDAPIEALRQSYPQGPTSLAAAFN
                     AIKSLEPKPDNIY"
BASE COUNT          437 a          873 c          901 g          368 t
ORIGIN      
        1 cgcaacttgc agtgagggac atgccgtgag tagtgaagga accccgatgc gttggttgag
       61 attggcggcg ccggcgctcc tgctcgccgc gtggtggacc ggttcgttcg cgcagcagca
      121 agggacgcct cccgctacga cgcctccgcc gacgccgccg ccggcgagca cgccgcagcc
      181 aagcacacag cggcccgcgc agcccgagca ggaaaaggaa gacgagaaac cgcaggcgcc
      241 gtccgacgat gccgcggacg aagagttcac gccaaccgag gagctgcagc cggatgcggc
      301 cgtcacgttc ccggtggata tctagccgcg agcaccatgg gcgccattgc catcgagatc
      361 aacgatgccg ggttggccgt cgccactgaa tccgacgtgc tcgccgtcga gcccggcttc
      421 gcgcgcatcg agggcggaaa aatcgtgacg ggcgagcagg cgcgagcgcg cgcacggcgg
      481 cagccgcgcc agaccagcag ccgattctgg agcgcgctca gcctggagcc gggctccgcc
      541 ggcgccgata tcggcaagag cgccgccgag ctcgcgttcg cgcagctcga gtcggtgtgg
      601 cagcgcgtcg gctccgacgc gacggccgtc attctcgtcg tgccgggcgg ctatcgcacc
      661 gagcagctcg gcctgctgct cggattggcg caggaatgcg gcatgcccgt gcgcgcgctc
      721 gtcgatgccg cggcggccgc gagcgtgcgg ccgtatccga agcggcagct cgtctatctc
      781 gacgcgagcc tttatcgcgt ctcgctgtcg ctgctcgagc agaacggcga agcgcaagtg
      841 cgcgcggagc acgcgctcgc gcaagggctc gccgcggtca cggacgcgtt cgcgcgccgc
      901 atcgcggatc acttcgtgcg cgcgacgcgt ctcgatccgt tcacgcacgc cgagacggag
      961 caggcgctct acgacgcctt gcccgagtgg ctcgaggctt tgcagcgcga ggagcgcttc
     1021 gagctcgcgc tgaaatatcg taacgaagaa tttcgagtta cggccgagcg cgacgccgtc
     1081 ctcggcgtcg ccaaagggcc gtatcgcgcg ttcattcagc tcgtggccca gcatcgtgag
     1141 cccggcaaga ggttggtcct gcagctctcc gaccgaatcg cggcgttgcc cgggctcgtg
     1201 ggcgagctct cgcggctcga cgactcgcag atcgagcgct atgcgcccgg ccatgccgcg
     1261 cgcagcgtgc tgctcgtgcc gtcgatcgcg gagagccaag gcgacgtgaa gctgctgaag
     1321 cgcgtgccgt ggcgcgaggc gccgagcgag gatgcttccg acgccgcgcg cagcgcagcg
     1381 gagcccagac cgatgctcgc gcgcgcgacg ccgccgacgc acgtcgtcta cggcggcttg
     1441 gcctatcgcg tcggcgcggc gggtctcgcg atcggccgcg aggccgatcc gcagcgccgc
     1501 acgatcgtgc tcacgggcag caatggcgtg tcgcggctcc attgcgaggt cgtcttgcgc
     1561 gacggcgagc tcaagctgcg cgacttgagc agctacggca cgttcgtcaa cgagcggaag
     1621 gtcgccggcg agacgacgtt gagccgcgcg gacgtcatcc gcataggctc gccgggtgcc
     1681 gagctgcacg tcgtcgacgt ggaggacgcc tgatggcgaa gcggcgcaag ggcgagctca
     1741 acgtattctc gatgtcgttc ctcgacgcga tcacggccgg attcggctgc gtcgtgctgc
     1801 tgttcatgct cgtcagcgcg aacgccgttc tcgaccagcg taccgccgtc gagaatcgcg
     1861 aggccgaagc ccgccggtgg gagctgcgcg tgctgaccgg gcaacgcaat ctcgtgcagc
     1921 tgcgcgaggc gctcgaggtc cagcttcgtg aatggaccaa cttgctcgcc gtccgccagg
     1981 gcctcgtgac gcaaatccaa gataccgagg ccgagctcgc gacgttgacg gaggactccg
     2041 aggcgcggcg tgcggcgatc gagcgcctgc gcaaggagct cgccgagctc aacaagcagt
     2101 cgcaggagct cgtgacgaac caacgcgagg agtccgacga cgctacgcgg ctgcgcgcgt
     2161 tccagggcga aggcaaccgc cagtatctga cgggcctgcg catgggcggc aagcacgtcg
     2221 tgattctcgt cgacacgtcg acgagcatgc tcgatcgcac gatcgtcaac atcttgcggc
     2281 gccgccacat gacgccggag cagcagcgcc gctcgccgaa gtggcggcag gtcgtcaaca
     2341 cggtcgactg gttgtcgacg cagatcgcgc ccgggaccga cgtccagatc atcggcttca
     2401 ccgacaaggc gacgtggctg attccgggca ccgaaggcaa atgggtcacc atcaacgacg
     2461 gcagcgagct cgacgcgccc atcgaggcgc tgcggcagag ctacccgcaa ggcccgacga
     2521 gcctggcagc ggcgttcaac gcgatcaagt cgctcgagcc gaagcccgac aacatctac
//
LOCUS       DASSNN010000705         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_355099, whole genome shotgun sequence.
ACCESSION   DASSNN010000705 DASSNN010000000
VERSION     DASSNN010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355099"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="VFL84_13240"
     CDS             <1..1260
                     /locus_tag="VFL84_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HET7609638.1"
                     /translation="ADEPPQVPGSVLSADPLDVACGEGVLRIHYGQREGGVYCSGAQL
                     ASELNMVEGLRFEPPRDRRAAAKRKTRVLILGVNGFIGNFLSERLLDDGRYEVHGMDL
                     NDGAIRRLESRPGFYFHEGDISIHREWIEYHIKKCDVIVPLVAIATPIEYTRNPLRVF
                     ELDFEENLRVVRYCVKYKRRIIFPSTSEVYGMCDDEEFDEDRSRLILGPINKQRWIYS
                     TSKQLLDRVIWAYGQQQGLKFTLFRPFNWIGPRLDSLEAARIGSSRAITQLILNLVEG
                     TPLLLIDGGRQKRCFTDVAEGIECLFRIIENRDGRCDGRIFNVGNPQNEASIRELAEL
                     LVEKFEKHPLRDRFPPLAGIKEVESQSYYGEGYQDVTHRRPSIANAKRTLGWQPVIPL
                     EESIERTLDFFLRDHLATVDGAESEPK"
     gene            1257..>2062
                     /locus_tag="VFL84_13245"
     CDS             1257..>2062
                     /locus_tag="VFL84_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005739438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7609639.1"
                     /translation="MTPLGLRIDVDTFRGTREGVPRLLDQLAAHGITGTFFFTLGPDN
                     MGRHLWRLLKPRFAMKMLRSRAASLYGWDILLAGTAWPGRRIGAHLAPVLRAADAAGH
                     ETGLHAWDHHRWQAAASRMSADALTREIRLGVEAFGDALGRRPDCSAAAGWVCNERAL
                     EAKESFGFRFNSDCRGRSIFAPVVNGRRCAPQVPTTLPTYDELIGRDGLTQEQYNERL
                     LSMIRPGELNVLTVHAEVEGIACAGLFERFLEACAARAIRPMTLGSLLREA"
BASE COUNT          356 a          714 c          647 g          345 t
ORIGIN      
        1 gcggacgagc cgccgcaagt gccgggcagc gtcctttccg ccgatccgct cgacgtcgcc
       61 tgcggcgaag gcgtgctccg aattcattac ggccagcgcg aaggcggcgt ctattgcagc
      121 ggcgcgcagc tcgcttccga attgaacatg gtggaaggct tgcgcttcga gcctccgcgg
      181 gaccgccgcg cggccgccaa gcgcaagacg cgcgtgctga tcctcggcgt aaacggcttc
      241 atcggcaatt tcctgagcga gcggctgctc gacgacggcc gctacgaggt ccacggcatg
      301 gatctcaacg acggcgcgat ccggcgcctc gagtcccgcc cgggcttcta cttccatgaa
      361 ggcgacatct cgattcacag ggaatggatc gagtaccaca tcaagaagtg cgacgtcatc
      421 gtgccgctcg tcgcgatcgc gacgccgatc gagtacacgc gcaatccgct gcgggtcttc
      481 gagctcgatt tcgaagagaa cctgcgcgtc gtacgctatt gcgtgaagta caagcggcgg
      541 atcatctttc cgtccacctc ggaagtctac ggcatgtgcg acgacgagga attcgacgaa
      601 gaccgctcgc ggctcatcct cgggcccatc aacaagcagc gctggatcta ttccacttct
      661 aaacaactgc tggatcgagt gatctgggcc tacgggcagc agcagggact caagttcacg
      721 ctcttcaggc cgttcaactg gatcgggccg cggctcgatt cactcgaagc cgcgcgcatc
      781 ggcagctcgc gcgccatcac gcagctgatc ctgaatctcg tcgagggcac gccgctgctg
      841 ctcatcgacg gcggccgcca gaagcgctgc ttcaccgatg tcgccgaggg catcgagtgc
      901 ctgttccgca tcatcgaaaa tcgcgacggg cgctgcgacg gacgcatctt caacgtcggc
      961 aatccccaaa acgaggcgag cattcgcgag ctcgccgagc tgctcgtcga aaagttcgag
     1021 aagcacccgc tgcgcgatcg ctttccgccc ctcgcgggca tcaaggaagt cgagagccag
     1081 tcctactacg gggagggtta tcaggacgtg acgcatcgcc gtcccagcat cgcgaacgcg
     1141 aaaaggacgc tcggctggca gcccgtcatc ccgctcgagg agtcgatcga gcgcacgctc
     1201 gatttcttcc tgcgcgacca cctcgcaacc gtcgatggcg ccgaatccga gccgaaatga
     1261 cgcctctcgg cctgcgcatc gacgtcgaca cgtttcgcgg cacgcgtgaa ggcgtgccgc
     1321 ggctgctcga ccagctggcc gcgcatggca tcacggggac tttcttcttt acgctgggtc
     1381 ccgacaacat gggccggcac ctctggcggc tcctcaagcc gcgcttcgcc atgaagatgc
     1441 tgcgctcgcg cgccgcgagc ctctacggct gggacattct gctggccggc acggcctggc
     1501 ctggcagacg catcggcgcg cacctcgcgc ccgtgctccg cgccgccgac gcggccggcc
     1561 acgagacggg cctgcacgcc tgggaccacc atcgctggca ggcagcggct tcgcgcatgt
     1621 cggcggacgc cttgacgcgc gaaatccgcc tcggcgtcga ggctttcggc gacgcgctcg
     1681 gccggcggcc ggattgctcg gccgcggcag gctgggtctg caacgagcgc gcgctcgaag
     1741 ccaaggaaag cttcggcttt cgtttcaaca gcgactgccg cgggcgcagt atcttcgcgc
     1801 ccgtggtcaa cggccggcgc tgcgcgccgc aggtccccac gacattgccg acctacgacg
     1861 agctcatcgg ccgcgacggg ctcactcagg agcagtacaa cgaacggctg ctctccatga
     1921 tccggcccgg cgagctcaac gtgctcaccg tacacgccga ggtcgagggc atcgcctgcg
     1981 cgggtctctt cgagcgattt ctcgaggctt gcgccgcgcg cgccattcgc cccatgactc
     2041 taggcagcct gcttcgcgag gc
//
LOCUS       DASSNN010000706         4013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1251730, whole genome shotgun sequence.
ACCESSION   DASSNN010000706 DASSNN010000000
VERSION     DASSNN010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251730"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFL84_13250"
     CDS             complement(<1..301)
                     /locus_tag="VFL84_13250"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609640.1"
                     /translation="MRRIALAALSMCALSANVALAHHSPVMFDQSKPLTLTGTVRQFQ
                     WTNPHSYIQLVVKNDAGQDEEWSLEMAAPTYLYNLGWRPSTVKSGDTLTVTIAPLR"
     gene            complement(304..1209)
                     /locus_tag="VFL84_13255"
     CDS             complement(304..1209)
                     /locus_tag="VFL84_13255"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7609641.1"
                     /translation="MTRTIVCWCLELAAALAVSCAAAQEAPLNVLSPKATSAGWTGVH
                     RPHTKLSDVLDRHKGGKDWSETVVDDESLTAQWISMAPGQKTPRRMNGDTREWWIVQS
                     GMLKFSVEGREPVIATKGFMVQVPYRTLYQIENVGTEAALRFEVNVTRARKLYPLDET
                     PTPLPGFDYIPTRVTGGRGTLDAQNRPVVDFAKVVSGEARGGPFVVDDRSFANIIIGN
                     YQKPQPGNKGHYHEEGSEFWLIMLGKIRYKIEGLPEFEAEPGDVVYVPRQTWHLASFG
                     GSEGLRSCRLAMNGFPYQAHMFEVD"
     gene            1356..2285
                     /locus_tag="VFL84_13260"
     CDS             1356..2285
                     /locus_tag="VFL84_13260"
                     /inference="COORDINATES: protein motif:HMM:NF028266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HET7609642.1"
                     /translation="MSSPSSSSRLISLLGLAVAALPAHADEYSWQLSANASRVETRGL
                     DTDSWAVDGTYYLNAIDDGDGPLALASFLNPTTHVSLEASKTELPLGFLDDPTVLALN
                     GAYVLPAQKWYVGADYTETSADDTGFFSRSESKGYGVEAGRYLGASTTLELGLGRFEQ
                     SSETPAACPPTVCFGTPLDADTTTDSVSLEVFHVRRFRSLTYSLQASVSRGEQQTDIR
                     SSVTSLPLASGGRVPVRTYSVAGELFPTKALGVRLGYTQIESAGYRGDSYDAATTWFF
                     KPRFAIQVGLSRASLELGPDKSDGATVRFIGRL"
     gene            complement(2376..2846)
                     /locus_tag="VFL84_13265"
     CDS             complement(2376..2846)
                     /locus_tag="VFL84_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HET7609643.1"
                     /translation="MKRIALATFVALGLAATATAQQTRTPPGNDRTNQVSFAEVDKDK
                     DGKLTREEGNAINGFDFSRADTNNDRTLTRQEFDAAMAKSTARGDGHEGPLTGDRTEQ
                     VTFEQVDKNKDGKIDRDEAEDVDGFNFSAADADDDRWISRAEFQTAMAGSRPRG"
     gene            3007..3420
                     /locus_tag="VFL84_13270"
     CDS             3007..3420
                     /locus_tag="VFL84_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET7609644.1"
                     /translation="MRSAAAAARSHVHAGVPQAQGGAAVYTLADPRDLQAPRYVGQTR
                     DPHARFAQHVRTARLWLPDEPPWWVRSPKHRPLYAWIRELHRDGGWLPFMWVEQWVGP
                     GADPRVAERAAIMRWLARGAQLLNVEARGPQLPLL"
     gene            complement(3406..3651)
                     /locus_tag="VFL84_13275"
     CDS             complement(3406..3651)
                     /locus_tag="VFL84_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609645.1"
                     /translation="MLSPTAKTKRFLEMIQIGLTFGSVCTVVDASLMPARAQDAAGWY
                     TASAHEAGASADSLFRDLARTERKRRESELREALEQR"
     gene            3815..>4013
                     /locus_tag="VFL84_13280"
     CDS             3815..>4013
                     /locus_tag="VFL84_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609646.1"
                     /translation="MTRFVRALIVAAACLVTGWWLLRAPEQERLSESTPARRGALPAR
                     EVESHASAAVHDAELVADVPSA"
BASE COUNT          635 a         1315 c         1313 g          750 t
ORIGIN      
        1 tgcgcagcgg cgcgatcgtc acggtcagcg tatcgccgct cttcacggtc gacgggcgcc
       61 aaccaaggtt atagagatag gtcggagccg ccatctcgag gctccattcc tcgtcttgcc
      121 cggcgtcgtt cttcacgacg agctggatgt agctgtgcgg gttcgtccat tgaaactggc
      181 gaaccgttcc cgtcagcgtc agcggcttgg actgatcgaa catcacgggc gagtgatgcg
      241 cgagcgcgac gttggcactc aacgcgcaca tcgagagagc ggcgagggcg atgcggcgca
      301 tgctcaatcg acctcgaaca tgtgcgcttg atacgggaag ccgttcatcg cgagccggca
      361 cgagcggagt ccctcgctgc cgccaaagct cgcgagatgc cacgtctgcc gcggcacgta
      421 cacgacgtcg ccgggctccg cctcgaactc gggcaggcct tcgatcttgt agcggatctt
      481 gccgagcatg atcagccaga actccgagcc ttcctcgtgg taatgccctt tgttgccggg
      541 ctgcggcttc tggtaattgc cgatgatgat gttcgcgaag ctgcggtcgt cgacgacgaa
      601 cgggccgccg cgcgcctctc ccgagacgac tttcgcgaag tcgacgacgg gccgattctg
      661 cgcatcgagc gtgccgcggc cgcccgtcac gcgcgtcgga atgtaatcga agccagggag
      721 cggcgtcggc gtctcgtcga gcgggtagag cttgcgcgcg cgtgtcacgt tgacctcgaa
      781 ccgcagcgcg gcctcggtgc cgacgttctc gatctggtaa agcgtgcggt acggcacctg
      841 caccatgaag cccttcgtcg cgatgacggg ctcgcgccct tcgacgctga acttcagcat
      901 cccggactga acgatccacc actcgcgcgt atcgccgttc atgcgccgcg gcgtcttttg
      961 ccccggcgcc atcgagatcc attgcgcggt gagactctcg tcgtcgacga cggtctcgga
     1021 ccaatccttc ccacctttgt gcctatcgag cacgtcgctc agcttcgtgt gcggccgatg
     1081 cacgcccgtc cagccggcgg atgttgcttt cggcgacagc acgttgagcg gcgcctcttg
     1141 ggcggccgcg cacgagaccg ccagcgccgc ggccagctcc aagcaccagc agacaatcgt
     1201 tcgcgtcacg gtgcgtctca tgggcgcctc tcgctgcaaa aatccccctt tcgcgatgaa
     1261 gcagcgtagt cgtctccccg gcgcgcggca atcgcggaac tcttcgcgac cggccctctc
     1321 gaactgcgca cggtcttggg aaggggggaa cgcgtatgtc gagtccttcg tcatcgtcga
     1381 ggctgatttc actgctaggt ctggccgtcg ccgcgcttcc ggcacacgcc gacgagtatt
     1441 cgtggcaact gtccgccaat gcgagccgcg tcgaaacgcg cggtctcgat acggatagct
     1501 gggccgtcga cgggacgtat tacctgaatg cgatcgatga cggcgacgga ccgcttgcgc
     1561 tcgcgtcgtt cctgaatcca acgacgcacg tgtcgctcga ggcgagcaag actgaattgc
     1621 cgctgggttt cctggacgac ccgaccgtcc tcgccttgaa cggcgcctat gtgctacccg
     1681 cgcagaaatg gtatgtcggt gccgattaca cggagacgag cgcggacgat acgggcttct
     1741 tttcacgctc cgagtcgaag ggctacggcg tcgaggcggg tcgctatctc ggcgcgagca
     1801 cgacattgga gctcggcctc ggccgattcg agcagagctc ggagaccccc gcggcttgtc
     1861 cgccgaccgt ttgcttcggg acgcccctcg acgccgacac gacgacggac tccgtcagcc
     1921 tcgaggtatt ccacgtgcgg cgatttcgtt cgctgaccta ctctttgcag gcaagcgtat
     1981 ctcgaggcga gcaacaaacc gatatccgct cgtcggtcac ttcgctgccg cttgcgtcgg
     2041 ggggccgtgt gcccgttcgc acgtattccg tcgccggcga gctgtttccg acgaaggcgc
     2101 tcggcgtgcg cctcggatac acacagatcg aaagcgcggg ctaccgcggc gacagctacg
     2161 acgccgccac gacgtggttc ttcaagccgc gcttcgcgat acaagtaggg ctgtcgcgcg
     2221 cgagcctcga gcttggaccc gacaaatccg acggcgccac ggtccgattc atcggtcgac
     2281 tgtagccgca gcgcgaacga gcgcgctgcg tccgaaggtg tggtgagcga gagcgcgggg
     2341 ccgcgcgatg cggccccgcg cgcttgccgg aagcgctagc cgcgcggccg cgatccggcc
     2401 atcgccgttt ggaactcggc gcggctgatc caacggtcgt cgtcagcgtc cgccgccgag
     2461 aagttgaagc cgtcgacgtc ttcggcctcg tcacggtcga tcttgccgtc cttgttcttg
     2521 tcgacttgct cgaacgtcac ttgctccgtg cgatcgcccg tcagcgggcc ttcgtggccg
     2581 tcgccgcgcg cagtcgactt ggccatcgcc gcatcgaatt cttgcctcgt cagcgtccga
     2641 tcgttgttcg tatcggcgcg tgaaaagtcg aagccgttga tggcgttgcc ttcctcgcgg
     2701 gtcagcttgc cgtccttgtc cttgtcgact tccgcgaagc tgacttggtt cgtgcggtcg
     2761 ttgcccggtg gcgttcgggt ttgctgcgcc gtagccgtcg cagcgagacc gagagccaca
     2821 aacgtagcga gtgcaatccg tttcatagca atacctccgt tccatggttc gacaacccgg
     2881 ttttgcaaga catatgccag aagcgcttcg gcgcaagtgc ctgagcgggc tcgaacaatg
     2941 tagctgtatc gatgtacagt ctcggcagga ggctcgcaaa cactggaaga agcgcaacgc
     3001 gttttcatga ggtcggcggc cgccgcggca cgctcccacg ttcatgccgg cgttccgcaa
     3061 gcgcaaggcg gcgccgccgt ctacacgctc gcggacccgc gcgacctgca agcgccgcgc
     3121 tacgtcgggc aaacacgcga cccgcacgcg cgcttcgcgc agcacgtgcg cacggcgcgc
     3181 ctgtggcttc ccgacgagcc gccgtggtgg gtgcggtcgc cgaaacatcg gccgctgtac
     3241 gcctggatac gcgagctcca tcgggacggc ggctggctgc cgttcatgtg ggtcgagcaa
     3301 tgggtcggcc ccggcgccga cccgcgcgtg gccgagcgcg ccgcgatcat gcgctggctc
     3361 gcccgagggg cgcagttgtt gaacgtcgag gcccgagggc cgcagctacc gttgctctag
     3421 cgcttctctg agctccgatt cacggcgctt gcgctcggtt cgggcaaggt cgcggaagag
     3481 ggaatctgcg ctcgctcccg cttcgtgggc gcttgccgtg taccagccgg ctgcgtcttg
     3541 cgcgcgcgcc ggcatgaggc tcgcatcgac gacggtgcag acgctcccga acgtcaggcc
     3601 gatctgaatc atctcgagga accgtttcgt ctttgccgtc ggggagagca tggactcgag
     3661 cgtaggtcgc ccgagatgaa tcgaacctga aaccgtcgac gacgtgaacg cgcctgttga
     3721 accaaggcgc gttgggaggc tcaaggcgga tactattctt gactccgcgc gcgagccgcg
     3781 tcgcgcctac tgtcgcgctg cgtcaagagt gaatatgacg agattcgttc gagcgctgat
     3841 cgtcgcggcg gcgtgcctcg tgaccgggtg gtggctgctg cgcgcgccgg agcaagagcg
     3901 gttgtcggag tccacgccgg cgcggagggg ggcgctaccg gcgcgagaag tcgagtctca
     3961 cgcgagcgcc gcggtgcatg acgcagagct cgttgccgac gttccttcgg cgg
//
LOCUS       DASSNN010000707         3757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1133702, whole genome shotgun sequence.
ACCESSION   DASSNN010000707 DASSNN010000000
VERSION     DASSNN010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133702"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VFL84_13285"
     CDS             <1..950
                     /locus_tag="VFL84_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HET7609647.1"
                     /translation="ALMFAGVEPMAAYALVGMGAAVYGPAKYGILPELVGSQRLVRAN
                     GWIEGSTILAIVLGTVVGSRVADYRISAAFMMVMGCYAASALATFGLPRLAPAQAGAE
                     AGIRVFIGRMRTLFVAARARFAMLGNSLFWASAAVMRLLLVIWAPAVLAARSSSDVAD
                     LTLPLALGIIAGALIVPRVIPIAHLRRARLAAYAMGACIVAFSLVDTVWPARAVLMAI
                     GFCGGMFLVPINAALQDIGHRSIGSGRAVAVQSFFENSAMLTGVGIYTAALGLGARPV
                     GSVVVVGVLVLIATTIVSWHLPPDPAVDAPGDDAAPAAQ"
     gene            complement(957..1394)
                     /locus_tag="VFL84_13290"
     CDS             complement(957..1394)
                     /locus_tag="VFL84_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7609648.1"
                     /translation="MSALRASLVVAAMLAAGTAAAQSTLESRREEVFTAERAFARTMA
                     ERDFAAFGRYVAADCVFFSGNTPLRGRDAVLAAWKAFFDGAQAPFSWEPDQVEVLASG
                     ELALSTGLVKDPAGAVAARFNTIWQRQPDGRWLVIFDKGGPAE"
     gene            complement(1398..1814)
                     /locus_tag="VFL84_13295"
     CDS             complement(1398..1814)
                     /locus_tag="VFL84_13295"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609649.1"
                     /translation="MSRQSFIGVMAAALAAAWVTPSRAHHSFSAEYDRDKPIKVTGTV
                     TKVEWTNPHARIYLDVKDADGNVVNWDFELGPPNGLMRQGWRRDSLHEGHVVTISGFL
                     SKDQQHVANARSVNLPDGRQVLAGSSFDIDAATAAP"
     gene            1903..2721
                     /locus_tag="VFL84_13300"
     CDS             1903..2721
                     /locus_tag="VFL84_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609650.1"
                     /translation="MTKLRRLYAALTLLAPIVASAQWGPHRDERIPRNPDGTPNLEAP
                     APRTADGKPDLTGVWQGFGTLGGTAAQQEPEGAVPRAGFANVGQNLKEPLPLRSEYKA
                     LADQRRADGGKDNPEAVCLPMGIMQFHTQGAPRKFIQTPDVLVILYEASMGVRQIFTD
                     GREFPDNDPQPWWYGYSVGHWEGDTLVVVTRGLRDGEWLDIFGTPLTDAATYTERFRR
                     VSYGRMEIDVTVDDPKAYTAPWTVRVNQRIMPEQELLEFVCNENNRYFVRGPKE"
     gene            2741..>3757
                     /locus_tag="VFL84_13305"
     CDS             2741..>3757
                     /locus_tag="VFL84_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609651.1"
                     /translation="MRARSNGLEPAKLALAALMLAAAAGCDGVTPTAPAAAAPADDGI
                     ANRNLRLVGRNDLQARSAYQPIVHAYGERRILFVGHHAGQALNPATGLIEKNGLSVVD
                     VTDPTRPTYLTHFPPTGTEASGTQHVQVCDGSALPNADDAKVYAIRTNGLLSYEVLDV
                     TDPAAPKFVTTIAETGVSSRPDSDRGNRETHKFQWDCATGIAYMNGTAAGWRVTRVLQ
                     AFDVSDPAHPKHVRDFGLVGYEPSSTEPYPKPQVAGLHQPFVVGNRMYLGYNSGEEGV
                     LQIVDRERFMSGDPEPTAANLRAPEIARIDLPSYWGVHTAKPIYDFEIADYADNSEAR
                     TRDLL"
BASE COUNT          597 a         1289 c         1273 g          598 t
ORIGIN      
        1 cggcactcat gttcgccggc gtcgagccga tggccgcgta cgcgctcgtc ggcatgggtg
       61 cggcggtcta cggtccagcc aagtacggaa ttctgcccga gctcgtcggc tcgcagcgtc
      121 tcgtgcgcgc gaacggctgg atagaaggat ccacgatcct cgcgatcgtg ctcggtaccg
      181 tcgtcggctc gcgcgtcgcg gactaccgga tatcggccgc tttcatgatg gtcatgggct
      241 gctatgcggc ttcggcgctt gcgaccttcg gcttgccgcg gctcgctcct gcgcaagcgg
      301 gcgcggaagc cggcatcaga gtcttcatcg gccgcatgcg aacgttgttc gtcgcggcga
      361 gggcccgctt cgcgatgctc ggcaattcgc tgttctgggc atcggctgcc gtgatgcgct
      421 tgctgctcgt catctgggcg cctgccgtgc tcgcggcgcg gagctctagc gatgtcgccg
      481 atctgacgct gccgctcgcg ctcggcatca tcgccggcgc gttgatcgtg ccgcgcgtga
      541 ttccgatcgc gcatcttcgc cgcgcgcggt tggccgccta tgcgatgggc gcgtgcatcg
      601 tggcgttcag cctcgtcgac acggtgtggc cggcgcgcgc ggtgctgatg gcgatcggtt
      661 tctgcggagg catgttcctc gtgccgatca acgccgcgct gcaggacatc ggccaccgct
      721 cgatcggcag cggacgcgcg gtcgcggtcc agagcttctt cgagaactcc gcgatgctga
      781 ccggcgtcgg catctatacg gccgcgctcg gcctcggcgc ccggcccgtc ggctcggtgg
      841 tcgtcgtcgg cgtgctcgtc ttgatcgcga cgacgatcgt ctcgtggcac ctgccgccgg
      901 atccggccgt cgacgcgccc ggagacgacg ccgcgccggc cgcgcagtga gctgcgttat
      961 tcggcgggtc cgcctttgtc gaagatcacg agccagcggc cgtcgggctg ccgctgccag
     1021 atcgtgttga agcgcgccgc tacggcgccc gccggatcct tgacgagccc cgtcgagagc
     1081 gcgagctcac ccgaggcgag cacctcgacc tgatcgggct cccacgagaa cggcgcttgc
     1141 gcgccgtcga agaatgcctt ccaagccgca agcacggcgt cgcggccgcg cagcggcgtg
     1201 ttgccggaga aaaagacgca gtccgcggcg acgtagcggc cgaacgccgc gaagtcgcgc
     1261 tcggccatcg tacgcgcgaa ggcgcgctcg gcggtgaaga cttcctcgcg ccgcgattcg
     1321 agcgtgctct gcgcggctgc cgtgcccgcg gcgagcatgg ccgcgacgac gagcgacgcg
     1381 cgcagcgcac tcacgggtta tggcgcggcc gtggccgcgt cgatgtcgaa ggacgagccg
     1441 gcgagcactt gccgcccgtc gggcaagttc acggagcgcg cgttcgcgac gtgctgttga
     1501 tctttcgaaa ggaagccgct gatcgtgacg acgtgcccct cgtgcagcga gtcgcgcctc
     1561 cagccttggc gcatgagccc gttcggcggg ccgagctcga agtcccagtt gacgacgttg
     1621 ccgtccgcgt ctttgacgtc gaggtagata cgggcgtgcg gattcgtcca ttcgaccttc
     1681 gtgacggtgc cggtcacctt gatcggcttg tcgcggtcgt actcggccga gaacgagtgg
     1741 tgcgcacggc tcggcgtgac ccacgcggcg gccaacgccg cggccatgac gcctatgaac
     1801 gactgacggc tcatcgactc ccccttcgac gtctactggc gcgatgatac gctctgaccg
     1861 gctttaagga ccatcgagcc ccgacccaca agagcgagaa cgatgacgaa gctccgacgt
     1921 ctgtacgccg cgctcacgct gctcgcgccg atcgtggcct cggcccaatg gggcccgcac
     1981 cgcgacgagc gaattccgcg caaccccgac ggtacgccga atctcgaggc gccggcgccg
     2041 cgcaccgccg acggcaagcc cgatctaacg ggcgtctggc agggcttcgg cacgctcggc
     2101 ggcacggccg cgcagcaaga gcccgaaggc gccgtgccgc gcgccggatt cgcaaacgtc
     2161 ggccagaact tgaaggagcc gttgccgctg cgctccgagt acaaggcgct cgccgatcag
     2221 cgccgcgccg acggcggcaa ggacaacccc gaggcggttt gtctgccgat gggaatcatg
     2281 cagttccaca cgcagggagc gccgcgcaaa ttcattcaga cgccggacgt gctcgtcatc
     2341 ctgtacgaag cgagcatggg agttcggcag atcttcacgg acggccgcga gtttcccgac
     2401 aacgatccgc agccgtggtg gtacggctat tcggtcggcc attgggaagg cgacacgctc
     2461 gtcgtcgtca cgaggggtct gcgcgacggc gaatggctcg acattttcgg cacgccgctg
     2521 acggacgctg ccacgtacac ggagcggttt cgccgcgtca gctacggacg catggagatc
     2581 gacgtgacgg tcgacgaccc gaaggcatac accgcgccgt ggaccgtgcg cgtcaaccag
     2641 cggatcatgc ccgagcagga attgctcgag ttcgtctgca acgagaacaa ccgctacttc
     2701 gttcggggtc cgaaagagta ggcggtcagg agcgcagccg atgcgcgcaa gatcaaacgg
     2761 cctcgagccg gcgaagctcg ccctcgcggc gctaatgctg gccgccgcgg cgggctgcga
     2821 cggcgtcacg cccaccgctc ccgcggctgc cgcgcctgcc gacgacggca tcgcgaatcg
     2881 caacctgcgg ctcgtcggcc gcaacgacct tcaggcgcgc tccgcatatc agccgatcgt
     2941 gcacgcctac ggcgagcgcc ggatactctt cgtcggccat cacgcgggcc aggctttgaa
     3001 tcccgcgacg gggctcatcg agaagaacgg gctctcggtc gtcgacgtca ccgatccgac
     3061 gcggccgact tacttgacgc actttccgcc gacgggcact gaagcgagcg gcacgcagca
     3121 cgtgcaagtc tgcgacggca gcgcgctgcc gaacgccgac gacgcgaagg tctacgcgat
     3181 ccgcacgaac ggcctgctga gctacgaggt gctcgacgtc acggatcccg cggcgccgaa
     3241 attcgtgacg acgatcgcgg agacgggcgt ctcgtctcgc cctgactccg atcgcggcaa
     3301 ccgcgagacg cacaagttcc agtgggactg cgcgaccggt atcgcctaca tgaacggcac
     3361 ggccgcgggc tggcgcgtca cgcgcgtgct gcaggcgttc gatgtctcgg atcctgcgca
     3421 cccgaagcac gtccgcgact tcggcctcgt gggctacgag ccgagctcga ccgagccata
     3481 cccaaagccg caagtcgcgg ggctgcacca gccgttcgtc gtcggcaacc gcatgtattt
     3541 gggctacaac agcggcgagg agggcgtgct gcaaatcgtc gaccgcgagc gattcatgag
     3601 cggcgacccc gagccgacgg cggcgaacct tcgcgcgccc gagatcgcgc ggatcgacct
     3661 gccgagctat tggggcgtgc acacggcgaa gccgatctac gatttcgaaa tcgccgacta
     3721 cgccgacaac agcgaagcgc gcacgcgcga cctgctg
//
LOCUS       DASSNN010000708         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1251768, whole genome shotgun sequence.
ACCESSION   DASSNN010000708 DASSNN010000000
VERSION     DASSNN010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251768"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VFL84_13310"
     CDS             complement(<1..743)
                     /locus_tag="VFL84_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HET7609652.1"
                     /translation="MTKLIREHATEYYAAKNFNIWYGFGVIATFVLVLQLVTGIFLTM
                     NYKPAATDAFASVEYIMRDVEWGWLIRYLHSTGASAFFIVVYLHMFRGLLYGSYKKPR
                     ELVWIIGMLIYLSLMGEAFFGYLLPWGNMSYWGAQVIVSLFGAVPVIGNELAEWIRGD
                     FYISDITLNRFFAFHVVAVPLLLVALVVVHIMALHEVGSNNPDGIEIKEHKGPDGKPI
                     DGVAFHPYHTSKDLVILIVFLIVFSAVVF"
     gene            complement(827..1417)
                     /gene="petA"
                     /locus_tag="VFL84_13315"
     CDS             complement(827..1417)
                     /gene="petA"
                     /locus_tag="VFL84_13315"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HET7609653.1"
                     /translation="MAESTGFTRRHFLTVATAVTGTAGAIAAAVPFVASFKPSARAQA
                     LGAPVTVDISKLEPGAMTRVEWRGRAIFVVNRTEQMLDTLTKDVDKLKDPDSEDSTQP
                     EDAKNPYRSIRPQYLVLEGVCTHLGCAPMARFEVAAADLGADWPGGFYCPCHGSKFDL
                     AGRVFVGVPAPTNLPVPPYRYINDSTILIGSDTGNS"
     gene            1614..>2005
                     /locus_tag="VFL84_13320"
     CDS             1614..>2005
                     /locus_tag="VFL84_13320"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7609654.1"
                     /translation="MPRIVEVASFVAQSVAIGLALAFVAVLVRPELISRAPASDDSQA
                     SYANAVAATAPAVVNIQTATVYSVPRTSRDLTLQGLGSGVVVDDEGYVVTNFHVIRDV
                     DQIVVQLADGRTAVPKLVGTDQETELALL"
BASE COUNT          393 a          644 c          628 g          340 t
ORIGIN      
        1 aagaacacga ccgccgagaa cacgatcaag aacacgatca agatgaccaa gtccttgctc
       61 gtgtgatacg gatggaacgc gacgccgtcg atcggcttgc cgtcggggcc cttgtgctcc
      121 ttgatctcga tgccgtcggg attgttcgag ccgacctcgt gcagcgccat gatgtggacg
      181 acgacgagcg ccacgagcag caacggcacc gcgacgacgt ggaacgcgaa gaagcggttc
      241 agcgtgatgt ccgagatgta gaagtcgccg cgaatccatt cggcgagctc gttgccgatc
      301 accggaaccg cgccgaatag cgacacgatg acctgggcgc cccaatagga catgttgccc
      361 cacggcaaga gatagccgaa gaacgcctcg cccatgaggc tcaaatagat gagcatgccg
      421 atgatccaga cgagctcgcg cggcttcttg tacgagccgt agagcaggcc gcggaacatg
      481 tgcaggtaga cgacgatgaa gaacgccgaa gcgcccgtcg aatgcaggta gcggatcagc
      541 cacccccact cgacgtcgcg catgatgtac tcgaccgacg cgaacgcgtc cgtcgccgcc
      601 ggcttgtagt tcatcgtcaa gaaaatgccc gtgacgagct gcagcacgag cacgaacgtg
      661 gcgatgacgc cgaagccgta ccagatattg aagttcttcg ccgcgtagta ctcggtcgcg
      721 tgctccctga tgagcttcgt catcgggaag cgagcgtcga tccatgcgcg gagagcggaa
      781 aggcctcggc cgagccgatc gaagacgtcc gcttcggtac tcgccattat gaattccctg
      841 tgtcggaacc gatcagaatc gtgctgtcgt tgatgtagcg atacggcgga acaggcaggt
      901 tcgtcggcgc agggacgccc acgaacacgc gaccggccag atcgaacttc gagccgtggc
      961 acgggcagta gaagccgccc ggccaatcgg cgccgagatc ggcggccgcc acttcgaacc
     1021 gcgccatcgg cgcgcagccc aagtgcgtgc agacgccctc gagcacgagg tattggggcc
     1081 gaatgctgcg atacgggttc ttggcgtcct cgggctgcgt cgaatcctcg gagtccggat
     1141 ccttcaactt gtcgacgtct ttcgtgagcg tgtcgagcat ctgctcggtg cggttcacga
     1201 cgaagatcgc gcgcccgcgc cactcgacgc gcgtcatcgc gcccggctcg agcttgctga
     1261 tgtcgaccgt aacgggggcc ccgagcgcct gcgcccgggc gctcggcttg aacgaagcga
     1321 cgaacgggac tgccgcggcg atcgcgccgg ccgtaccggt cacggccgtt gcgaccgtca
     1381 gaaaatgccg cctagtgaaa ccggtgcttt cagccacaaa gcacctcttg accgacgggg
     1441 tcaagcatca ggaagcctcc ctcaaaacca agttgagttc cattccgcac gggtgtcgca
     1501 cgtggtcctt tcgtacgccg cgcggtcggt tcttgcggat gcgcggcatt atacacagcg
     1561 gccaaacggc ttggccacaa agcacccgtg ccgctacctg ctagacttcc gccatgccgc
     1621 ggattgtcga ggtcgcttcc ttcgtcgcgc aatcggttgc gatcggcctc gctctcgcgt
     1681 tcgtcgccgt gctcgtgcgg cccgagctca tcagccgcgc gccggcgtcc gacgacagtc
     1741 aagcgagcta cgcgaatgcc gtcgccgcca cggccccggc cgtcgtgaac atacagaccg
     1801 cgactgtcta cagcgtgccg cgcacctcgc gcgatctgac cttgcaaggg ctcggctccg
     1861 gcgtcgtcgt cgatgacgaa ggctatgtcg tgacgaactt tcacgtgatc cgcgacgtcg
     1921 atcagatcgt cgtgcagctc gcggacggac gaactgccgt gccgaagctc gtcggcacgg
     1981 atcaagagac ggagctcgcg ctgct
//
LOCUS       DASSNN010000709         5415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_250752, whole genome shotgun sequence.
ACCESSION   DASSNN010000709 DASSNN010000000
VERSION     DASSNN010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250752"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFL84_13325"
     CDS             complement(<1..473)
                     /locus_tag="VFL84_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7609655.1"
                     /translation="MTDAQSINGDLAVRVAVDTAAMEWTPSPSRTVFRKRVHLVGPPE
                     SGQVTSVVRYEPSSTFHGHGHPDGEEILVLDGVFSDEHGDWPRGTYLLNPEGFRHAPF
                     SRDGCVLFVKLRQFPGRAREHVALRTESMPWEPARAGVAVRRLYAQPGFADSMRL"
     gene            complement(501..1091)
                     /locus_tag="VFL84_13330"
     CDS             complement(501..1091)
                     /locus_tag="VFL84_13330"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HET7609656.1"
                     /translation="MRALLENWRCRISFAWYCLDKGAVAFYSLLPLPLVLLAVVAYFL
                     REDLELVRAGHARTVAEAAAARDAELTCLAENVYYEARGEPLEGQYAVAEVTMNRVAS
                     SRFPKSVCEVVHEQRWDARRKRQVGAFSWTELDSLGRPRGAAWQQALEVATAVYDGTH
                     TPRVPNALYYHAKYIKPSWAKPNRRVATIGSHVFYR"
     gene            complement(1260..4265)
                     /locus_tag="VFL84_13335"
     CDS             complement(1260..4265)
                     /locus_tag="VFL84_13335"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609657.1"
                     /translation="MKRLLAGCAIVALAVIPLAAPAQQTPITRFARFTGTINFVATGG
                     SLRTQPNTGDSCAVGASSSQALTGIPAGASIVAAYLYWGGSGAAVDANVTLNGQAVAA
                     PRTFTATFNNGGTNFPYFGGFADITSRVTGNGAMTFSGLTVTTGAPHCGSSAVVAGWS
                     VVAIYGSASERLRAINVYDGLQFFRGNNLLLNPDGFRIPPTNFDGRFTVVTWEGDPGN
                     STPMNGFSEVLRFNGTAVDDGIVVAGSDPVIQPYDGTVNSAGVATSYGVDVDTFDVTA
                     LLAPGQTSATTEYSAGGDLVLLTAQIVSATSEPVVDLTLTKTHAGNFTVGGSNVYTLT
                     VSNLAGNQREDNTVVVTDTLPAGLTFVSGVGTGWTCGASGQTVTCSHPPILDPGNSLP
                     PITMTVAATGVAVPSVTNTATVTSASHDIDAANNTASDLAVVVGPNLSTSTKTVVDLN
                     GGEPDAGDTLRYTVTLIESAGLPAPGASVVDDLAAELGNLAVVSMPPGAANFSTGAGT
                     GANGTGRLDVRNIFVPANGSATVVFDAQIAAGTTPGTPIDNTATVTNLGGLGAAPSAP
                     TLIVSPSGIPSSGAKPLYLRSTPGNTLSRTPPGADGPVTLVFNTPVAWTMTPTLQLPM
                     TLPGGNIAVPLYLLRVGAGGTRTLQVTLSNTVTGTIGSATQTIALNTAGPPQLATFVI
                     ANPPTVTFPLGSAFRVTIRQTAPNNANRNTLVYPVGSAGNYSRVVLNSATVIDVNSVQ
                     TYGAAYPGGAAQANFNYGGTAYIRAVVSDPFGSFDIASARISIVDSASVVRVANAVMP
                     MVADSGAATRTYEYAYVVPAGSPSGGWSAQVTAFEGTEGLVTHTRTGGFVVPPLLPAL
                     RVTKTVDVLSDPVHGAVNPFQIPGSLQRYRVTVTNTGVGSVDASTLVIDDLVPANTDL
                     IVASGGGDPVQFFDGPTPSGLTFDYATHVTYSDQPGGAPPYGYTPTADGNGVDPSVTA
                     LRIAPSGAMPGAAGPNQPSFSVEFRVRVR"
     gene            4547..>5415
                     /locus_tag="VFL84_13340"
     CDS             4547..>5415
                     /locus_tag="VFL84_13340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609658.1"
                     /translation="MHAIGTAAGTNITSTATVNYSVGGTPATESSNTATVVVAELINV
                     TMALQSPTVSVAAGATNEALLFLVTNTGNGGETFTLLGDSVLVGDDFDPAPAAPFIYF
                     DTDSSGDLSPADTPYVAGSNDPVIAADGSVAVLLVNDIPAGLPDGQYGRSELTATSNT
                     GTGAPGTVMAGQGTGGVDAVIGTSGGEVAVFGQYLIGDILVSAVKSQAVQDPFGGSQP
                     IPGAAITYQVVVTATGSGVALGAALTDPIPASTTYVAGSLRLNGTPLTDAADGDAGSF
                     VLGPPRVAVSLGDL"
BASE COUNT          848 a         1837 c         1875 g          855 t
ORIGIN      
        1 tcgagccgca tcgaatcggc gaaaccgggc tgcgcgtaca gccgccgcac ggcgacgccc
       61 gcgcgcgccg gctcccacgg catcgattcc gtgcgcagcg cgacatgctc gcgcgcgcgg
      121 cccggaaact gccgcagctt cacgaacagc acgcagccgt cgcgcgagaa cggcgcgtgc
      181 cgaaagccct cggggttcag cagatacgtg ccgcgcggcc aatcgccatg ctcgtccgag
      241 aacacgccgt cgagcacgag gatctcctcg ccgtccgggt gaccgtggcc gtgaaacgtc
      301 gagctcggct cgtagcgaac gaccgacgtc acctgccccg actcgggcgg accgacgaga
      361 tgcacgcgct tgcgaaagac cgtgcggctc gggctcggcg tccattccat cgccgccgtg
      421 tcgacggcga cgcgcacggc aagatcgcca ttgatcgatt gcgcgtcagt cacgcgcact
      481 aaggtacaag acgcgagcgc tcagcgatag aacacgtggc tgccgatcgt cgcgacgcgg
      541 cggttcggtt tcgcccagct cggcttgatg tacttggcgt gataatagag cgcgttcggc
      601 acgcgcggcg tatgcgtgcc gtcgtagacg gccgtcgcga cctcgagcgc ctgctgccat
      661 gccgcgccgc gcggccggcc cagcgagtcg agctccgtcc acgagaacgc gccgacttgc
      721 cgcttgcgcc gcgcgtccca gcgctgctcg tgcacgacct cgcagacgga cttcggaaag
      781 cggctcgacg cgacgcggtt catcgtgacc tcggcgacgg cgtattggcc ttcgagcggc
      841 tcgccgcgcg cctcgtaata gacgttctcg gcgagacagg tgagctcggc gtcgcgcgcc
      901 gcagcggcct ccgccacggt gcgcgcgtgg cccgcgcgca cgagctcgag atcctcgcgc
      961 aagaagtacg cgaccaccgc gagcagcacg agcggcaacg gcagcagcga gtaaaacgca
     1021 accgcccctt tgtcgaggca gtaccacgcg aacgaaattc ggcagcgcca gttttcgagc
     1081 agcgcgcgca cgagacctcc gaaccaacgg ttcctgcgcg agcgcaggag ccgtcgagtg
     1141 tgtgccttcg tgggtgtgcg cgcagaatcg tctgcgggcg catggctgtc aaacgactgc
     1201 gtcgaatcgc cgactgctcg tgcagctcgc cgctttatgt gaagcgactc cgtctcacct
     1261 caacgaacgc gaacgcggaa ctcgacgctg aagctcggct gattcgggcc ggccgctccc
     1321 ggcatcgcac cgctcggcgc aatgcgcagc gccgtgacgc tcggatcgac gccgttgccg
     1381 tcggccgtcg gcgtatagcc gtacggaggc gcgccgcccg gctggtccga gtaggtcacg
     1441 tgcgtcgcgt agtcgaacgt gaggccgctt ggtgtggggc cgtcgaagaa ttgcacggga
     1501 tcgccgccgc cgctcgcgac gatgaggtcg gtgttcgcgg gcacgaggtc gtcgatgacg
     1561 agcgtgctcg cgtcgaccga tccgacaccc gtgttcgtga ccgtgacgcg gtagcgctgc
     1621 aagctgcctg gaatctgaaa cggattgacg gctccgtgca cggggtcgga cagcacgtcg
     1681 acggtcttcg tgacgcgcag cgcgggcaga agcggcggca cgacgaagcc gccggtgcgc
     1741 gtgtgcgtca cgagaccttc cgtgccttcg aatgccgtga cctgcgccga ccaaccgcct
     1801 gacggcgagc ccgccgggac cacgtaggcg tattcgtacg tgcgagtcgc ggcgccggaa
     1861 tccgcgacca tcggcatcac cgcgttcgcg acgcgcacga cggacgcgga gtcgacgatc
     1921 gagatgcgcg cggacgcgat gtcgaagctg ccgaatggat cgctgacgac cgcgcggatg
     1981 tacgccgtgc cgccgtagtt gaagttggct tgcgcggcgc cgcccgggta cgcggcgccg
     2041 tacgtctgca cgctgttgac gtcgatgacc gtcgcgctgt tcagcacgac gcggctgtag
     2101 ttgccggcgg aaccgacggg atacacgagc gtgttgcggt tcgcgttgtt cggcgcggtc
     2161 tgcctgatcg tgacgcggaa cgccgagccc agcgggaacg tcaccgtcgg cggattcgcg
     2221 atgacgaacg tcgcgagctg gggcggcccc gccgtgttga gcgcgatcgt ctgcgtcgcg
     2281 ctgccgatcg tgccggtcac ggtgttcgag agcgtgactt gcagcgtgcg cgtgcctccg
     2341 gcgccgacgc gcagcagata gagcggcacg gcgatgtttc cgcccggcag cgtcatgggc
     2401 agttgcagcg tcggcgtcat cgtccatgcg accggcgtgt tgaacacgag cgtcacgggc
     2461 ccgtcggcgc cgggcggcgt gcgcgataac gtattgcccg gcgtgctgcg cagatagagc
     2521 ggcttcgcgc ccgacgacgg aatgccggac ggcgagacga tcagcgtcgg cgcggacggc
     2581 gccgcgccga ggccgcctaa gttcgtgacc gtcgcggtgt tgtcgatcgg cgtgccgggc
     2641 gtcgtgcccg ccgcgatctg cgcgtcgaac acgaccgtcg cgctgccgtt cgccggcacg
     2701 aagatgttgc ggacgtcgag gcgccccgtc ccgttcgctc cggtgccggc gccagtcgaa
     2761 aaattcgccg cgccgggcgg catgctgacg acggccaagt tgccgagctc ggccgcgagg
     2821 tcgtcgacga cactggcgcc gggcgccggc agacccgcgg actcgatgag cgtgaccgtg
     2881 tagcgcagcg tgtcgccggc gtcgggctcg ccgccgttca agtcgacgac ggttttcgtc
     2941 gacgtcgaga ggttcgggcc gacgacgacg gcgaggtccg acgccgtgtt gttcgccgca
     3001 tcgatgtcgt ggctggcgct cgtgacggtg gccgtgttcg tgacgctcgg cacggcgacg
     3061 ccggtcgccg cgaccgtcat cgtgatcggc ggcagcgagt tgccgggatc gagtatcggc
     3121 ggatgcgagc acgtgacggt ctgacccgag gcgccgcacg tccagcccgt gccgactccg
     3181 ctcacgaacg tgagcccggc gggcaacgtg tcggtcacga cgacggtgtt gtcctcgcgc
     3241 tgattgccgg ccaggttcga gaccgtcagc gtgtagacgt tgctgccgcc gaccgtgaag
     3301 ttgccggcgt gcgtcttcgt caacgtcaga tcgacgacgg gctcgctcgt cgcgctgacg
     3361 atttgcgcgg tcaagagcac gaggtcgccg cccgccgagt actccgtcgt cgccgacgtc
     3421 tgacccggcg cgagcagcgc ggtgacgtcg aacgtgtcga cgtcgacgcc gtagctcgtc
     3481 gcgactcccg ccgagttgac ggtgccgtcg tacggctgga tcacggggtc gctgcccgcg
     3541 acgacgatgc cgtcgtcgac ggcggtgccg ttgaagcgca gcacctcgct gaagccgttc
     3601 atcggcgtcg agttgccggg gtcgccttcc cacgtgacga ccgtgaaccg gccgtcgaaa
     3661 ttcgtcggcg gaatccggaa cccgtcggga ttcaacagca ggttgttgcc gcggaagaat
     3721 tgcaggccgt cgtagacgtt gattgcgcgc agcctctcgc tcgcgctgcc gtagatcgcg
     3781 acgacgctcc agccggcgac gacggcgctg ctgccgcagt gcggcgcgcc cgtcgtcacc
     3841 gtgagccccg agaacgtcat cgcgccgttg ccggtgacgc ggctcgtgat gtcggcgaag
     3901 ccgccgaagt acggaaagtt cgtgcctccg ttgttgaacg tcgccgtgaa cgtgcgcggc
     3961 gccgcgacag cctggccgtt cagagtcacg ttcgcgtcga cggccgcgcc ggagccgccc
     4021 cagtacagat acgccgcaac gatcgacgcg ccggccggaa ttcccgtcaa cgcctgcgag
     4081 ctcgacgcgc cgacggcgca agagtcgccc gtgttcggct gtgtgcgcaa cgagccgccg
     4141 gtcgcgacga agttgatcgt cccggtgaaa cgcgcaaacc gagtgatcgg cgtttgctgt
     4201 gccggcgccg cgagcggaat taccgcgagc gcaacgattg cgcatcctgc gagcagtcgc
     4261 ttcatgcgac gcacttaaca atgtgaccgg ccgtcttggc cgaacgctac tgtgagctac
     4321 cccacatgga tatccgcgac gctttcggca gttttttcgc ggcccttcct ttaccgttct
     4381 caaaccttga acggattcac agggtgaacg tcgcgcatga cgcgcggcga attcgcatgg
     4441 acgcgagaca gtgctcacaa gactgacgtc atcgtcgcgc tcgcgcgacg cgcttcgacg
     4501 cgctctactc ctcacgctcg tctcaagcgc tgcgtcaccc gcggccgtgc acgctattgg
     4561 cacggccgcg ggcactaaca tcacgagcac tgccaccgtc aactactcgg tcggcggcac
     4621 gccggcgacg gagagctcga atacggcgac cgtcgtggtc gcggagctca tcaacgtcac
     4681 gatggcgctg caaagcccga cggtctccgt cgcggccggc gctacgaacg aagcgctgct
     4741 gttcctcgtg acgaacacgg gcaacggcgg cgaaacgttc acgctcttgg gcgacagcgt
     4801 gctcgtcggc gacgacttcg atccggcgcc ggccgcgcca ttcatctatt tcgacacgga
     4861 ctcgagcggc gacctttcgc cggccgatac gccgtacgtc gccggcagca acgaccctgt
     4921 gatcgccgcg gacggcagcg tcgccgtgct gctcgtcaac gacataccgg ccggcttgcc
     4981 ggacggccaa tacggccgca gcgagctgac cgcgacgtcg aacacgggca ccggagcgcc
     5041 gggcacggtg atggcggggc aaggcaccgg cggcgtcgac gcggtcatcg gcacgagcgg
     5101 cggtgaagtc gcggtgttcg gccagtacct gatcggcgac attttggttt ccgccgtgaa
     5161 gtcgcaagcc gtgcaggatc cgttcggcgg gagccagccg ataccgggcg cggcgatcac
     5221 ttatcaagtc gtcgtgaccg cgaccggctc gggggtcgcg ctcggcgccg cgctcaccga
     5281 cccgattccg gcgagcacga cctacgtcgc cggcagcttg cgcttgaacg gcacgccgct
     5341 gacagacgcc gccgacggcg acgccggctc gttcgtcttg ggaccgccgc gcgtcgccgt
     5401 gagcctcggc gacct
//
LOCUS       DASSNN010000710         3349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_121354, whole genome shotgun sequence.
ACCESSION   DASSNN010000710 DASSNN010000000
VERSION     DASSNN010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121354"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1462)
                     /locus_tag="VFL84_13345"
     CDS             complement(182..1462)
                     /locus_tag="VFL84_13345"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7609659.1"
                     /translation="MADSAPRSEQKLDILFVTSDLFPPFRPAAKSVFAEGLAARGHRI
                     DWLIQAAEPTSRAGEQPFKGGVAYVAPTNAGNTRLARLKRHLADAWNDFRVFGLLRRR
                     RYSLVQIKDKYFGALVAIAAAKLHGVPVFYWLAYPHGEASLYAARSGVARYRWLYRWR
                     GALQRFVLYRIIMPACTHVFVQSEQMRRDVAGEGIPLELMTAVPSSLNLRDIDALAGA
                     GEDEPSGPPTVVYLGTLLRERQLDFIVRAHARVVSALPDAQLVFVGSGWMPDDEQLLR
                     REAERLGLGGNVTITGWLPMRDAWRHVRRAALCVSPYLPVPILRSTSPTKLIEYMALG
                     KAVVANDHPEQADVLRASGAGLVCAWDEQEFAAAIVELLRDPERCKSMGAAGRRYVAE
                     HRTHWAMVDLVAGRYRQHLREPPKPPKPRAFARV"
     gene            complement(1455..2159)
                     /locus_tag="VFL84_13350"
     CDS             complement(1455..2159)
                     /locus_tag="VFL84_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609660.1"
                     /translation="MGDQPFLTNLDEYPGALLVAGCDWSATTAITRMFRRLPCFADSS
                     WGHDDELDGALLLAGLSPRRTAGRPCLQTSYVRDRYREYFAHDDFTLVWIVREPRAAV
                     RSLLASRDRALPARNALGLSAKSEGGQGASRLEKACAAYVASIRQTTELKERLGDRVA
                     VVDYDELAADRNRLLPALCRFAEVGCDSHVLRHLHGKSVRKGSLASFEATIVDQLALP
                     SYRRARSAATLSLAHG"
     gene            complement(2405..>3349)
                     /locus_tag="VFL84_13355"
     CDS             complement(2405..>3349)
                     /locus_tag="VFL84_13355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7609661.1"
                     /translation="RRLCHEVLSEGQHRPRASVDTAYSDELVRLILNAKGPGALCDEI
                     MRDEDPSYLRRHLELTVTAHVDPAELEDADVLDFGCGAGASTVILAKLLPRSRITGIE
                     LRRANLEVARARAEFYGLESTRFLLAPSGERLPGGLGRFRAIMLSAVFEHLLPAEREA
                     LLPSLWRLLSPGGVLFVDETPSRWFPIETHTTGLPLINYLPDRLAETYARRCSRRVRR
                     DATWDEMRRDGIRGATANGILGLLPADAGRPVLLKPRRLGITGALDLWTRGYAAGGRG
                     WRGALKAAAAPPLRSAARLLDGSWLVPYLSLAIRKSPA"
BASE COUNT          561 a         1143 c         1149 g          496 t
ORIGIN      
        1 tggcgcgagc gccgcccatg aggcggatga gcacgacgag catcgcgatc gccgacagcg
       61 cctcgggcat caggatcaga tacgggtgca gcagcttacg cgattcgatc aggtactcga
      121 cgaccaggat ttgaatcagc agcgcgatga cgagcccgtg catggccggc gactctctgc
      181 gtcaaacgcg tgcgaacgct ctcggcttcg gcggttttgg cggctcgcgc aggtgctggc
      241 gataacggcc ggcgacgaga tcgaccatag cccaatgcgt gcgatgctcg gcgacgtaac
      301 ggcgcccggc cgctcccatg ctcttgcaac gctcggggtc gcgcagcagc tcgacgatcg
      361 ccgcggcgaa ctcctgctcg tcccacgcgc agacgagacc cgcgccgctc gcgcgcagca
      421 cgtcggcttg ctcgggatga tcgttcgcga caaccgcttt gccgagcgcc atgtactcga
      481 tgagcttggt cggcgacgtc gagcgcagga tcggaaccgg caaatacggc gagacgcaca
      541 gcgctgcgcg ccgaacgtgc cgccacgcgt cgcgcatcgg cagccaaccc gtgatcgtga
      601 cgttgccgcc gagcccgagc cgctcggctt cgcggcgcag cagctgctcg tcgtcgggca
      661 tccagccgct gcccacgaat acgagctgcg catcgggcag cgcgctcacg actcgcgcgt
      721 gcgcacgcac gatgaagtcg agctgccgct cgcgcagcag agttccgagg tacacgaccg
      781 tcggcggccc gctcggctcg tcttcgcctg cgccggcgag cgcgtcgatg tcgcgcaggt
      841 tcagcgagct cggcacggcg gtcatgagct cgagcgggat gccttcaccg gcgacgtcgc
      901 ggcgcatctg ctcgctctgc acgaacacgt gcgtgcaggc gggcatgatg atccgataca
      961 gcacgaagcg ctgcagcgcg ccgcgccaac ggtagagcca gcgatagcgc gcgacgccgc
     1021 tgcgggcggc gtacaacgag gcttcgccgt gcggataggc gagccagtag aacaccggca
     1081 cgccgtggag cttcgcggcc gcgattgcga cgagcgcgcc gaaatacttg tccttgatct
     1141 gcacgagcga atagcggcgc cgtctcagaa gcccgaacac tctgaagtcg ttccacgcgt
     1201 cggcgagatg ccgcttgagc cgcgcgaggc gagtgttgcc cgcgttcgtc ggcgcgacgt
     1261 acgcgacgcc gcccttgaac ggttgctcac ccgctcggct cgtcggctcc gcggcctgaa
     1321 tcagccaatc gatgcgatgc ccgcgagccg cgagcccttc cgcgaagacc gatttggcgg
     1381 cagggcgaaa cggcggaaat aggtccgacg tcacgaacaa gatgtcgagc ttctgctcgc
     1441 tgcgaggggc cgaatcagcc atgggccaag ctcaacgttg cggccgaacg ggcgcgccga
     1501 tacgacggca gcgcgagctg atcgacgatc gtcgcttcga agctcgcgag cgagcctttg
     1561 cgcacactct tgccgtgcag gtgccgaagc acgtgagaat cgcagcccac ctccgcgaag
     1621 cggcacaacg cgggcaacaa gcggttgcgg tcggcggcga gctcgtcgta gtcgacgacc
     1681 gcgactctat cgccgagacg ctccttgagc tccgtcgtct gccggatgct cgcgacgtac
     1741 gccgcgcacg ccttctcgag ccgcgacgct ccttggccgc cctcggactt ggcggacaaa
     1801 ccgagcgcgt tccgtgcggg cagcgcgcgg tcgcggctcg cgagcaacga ccgcaccgcc
     1861 gcgcgcggct ctcgtacgat ccacacgagc gtgaaatcgt cgtgagcaaa gtactcgcga
     1921 tagcggtcgc gtacgtagct cgtctgaaga cacgggcgcc cggccgtgcg gcgcgggctc
     1981 aagccggcga ggagaagagc gccgtcgagc tcgtcgtcgt gtccccacga ggaatcggcg
     2041 aagcacggca gccgcctgaa catgcgcgtg atcgcggtcg tggccgacca gtcgcagccc
     2101 gcgaccagca gcgctccggg gtactcgtct aagttcgtca agaacggctg atcgcccatg
     2161 gccgcgtaaa agtccgacca ggagctgatt ccatggtcgc cgcgacggtg gacgagctgc
     2221 gtgaagtgag cgcggcgctt gtgtcgggct agggccggtc gaagcgacgc gtcggcggcg
     2281 atggtcacca cgttgcgcga tcccagggtg ttgttgaatc cacgtcatcg atgcaagtcc
     2341 catgccttcg gcgaactgcc gccgcggcgg cggcgagttg cgccggcttt ccaaaaactg
     2401 ctcgttacgc gggcgacttg cggatcgcga gcgacaggta cgggacgagc caggagccgt
     2461 ccaacagacg cgcggcgctt cgaagcggcg gtgccgcggc tgccttcagc gccccgcgcc
     2521 agcccctgcc gcccgcggcg tagccgcggg tccagagatc gagggctcca gtgatgccga
     2581 gccgtcgcgg cttcaagaga accgggcggc cggcgtcggc cggcagcagg ccgaggattc
     2641 cgtttgccgt cgcgccgcgg atgccgtcgc ggcgcatttc atcccaagta gcgtcgcgcc
     2701 gcacgcggcg cgagcatcgc cgagcatatg tttcggcgag ccgatcgggc aggtagttga
     2761 tcagcggcag gcccgtcgta tgagtctcga tcggaaacca ccgcgacggc gtctcgtcga
     2821 cgaacagcac gccgcccggt gagagaaggc gccacagcga cggcagcagc gcctcgcgct
     2881 cggctggcag cagatgctcg aagacggcgc tcagcatgat cgcgcggaac cggccgaggc
     2941 cgcccggcaa ccgctcgccg ctcggcgcga gcaggaatcg cgtgctctcg agcccgtaaa
     3001 attcggcgcg cgcgcgcgcg acctcgagat tcgcccggcg cagctcgata ccggtaatcc
     3061 tgctcctcgg cagcagcttc gcgagtatga ccgtcgaggc gccggcgccg cagccgaagt
     3121 cgaggacgtc ggcatcctcg agctccgcgg gatccacgtg cgcggtcacc gtcagctcga
     3181 gatgccgccg caagtagctc gggtcctcgt cgcgcatgat ctcgtcgcat agcgcgcccg
     3241 gccctttggc gttcagaatc agccgcacga gctcgtccga gtaggcggtg tcgacgctgg
     3301 cccgcggtcg gtgttgccct tcgctcagca cttcgtggca gagccggcg
//
LOCUS       DASSNN010000711         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_860989, whole genome shotgun sequence.
ACCESSION   DASSNN010000711 DASSNN010000000
VERSION     DASSNN010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860989"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VFL84_13360"
     CDS             complement(<1..905)
                     /locus_tag="VFL84_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7609662.1"
                     /translation="MIRHYARAAAATGIALAAFACGAQSTRSYSAVTDARLESPEPRN
                     WLMFLREYNSWSYSPLREINTRNVANLVPVWTMSTGTAEGHQSPPIVNDGVMFITTPF
                     NQVIAIDAATGDLLWRYRHTIPADIRMGHPTNRGVALYGDKVYTATSDARVVALDAKT
                     GAVVWNKAVESYNTGYYMTIAPLAARGKIMVGVSGGERGIRGFVVALDANTGEQLWKT
                     YTVPAPGEPGNDTWPGDSWRTGGAPVWVTGSFDPKLGLTYWGTGNPGPWTGDTRPGDN
                     LYTNSVIALDVETGALKAHHQYHWN"
     gene            complement(916..1533)
                     /locus_tag="VFL84_13365"
     CDS             complement(916..1533)
                     /locus_tag="VFL84_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET7609663.1"
                     /translation="MPDRRETAVAYRNTPRRRVLKACCGLALCAPVAARGDDLDDAAA
                     PPQAGDALVFAYGDRAGQVIAPADVALGAKQILAYPMQPATRRVRDGSRLNQLIVVRL
                     PPDKLIDATRARAADGVVAYSGVCTHTGCDVTDWFGDTLHFKCPCHESEFDPGDGARV
                     VGGPAPLPLAALPIKLVDGALVVAGPFEGRVGFQALDPFSAAPPQ"
     gene            1606..>2343
                     /locus_tag="VFL84_13370"
     CDS             1606..>2343
                     /locus_tag="VFL84_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HET7609664.1"
                     /translation="MARSVKTSVVGSYPQPDWLIDRARLTKMMAPRIRVREVWRVPPD
                     RLEQAQDEATLEAIRDQERAGVDIITDGEIRRESYSNKFANALDGIDLTRVGEIVGRT
                     GNRIPVPIVSGPIRRRGPVEVRDVEFLRKNTSKTIKITLPGPFTMAQQAVNEHYRDDE
                     SVALAYAEAVNEEVHDLFAAGADVVQLDEPWLQARAEAARRYGIKAINRALAGVRGTT
                     ALHLCFGYAALVKDKPASGYSFLAELEE"
BASE COUNT          364 a          775 c          845 g          359 t
ORIGIN      
        1 tcgttccagt gatattggtg atgagccttc agcgcgcccg tctcgacgtc gagcgcgatg
       61 acggagttcg tgtagaggtt gtcgccgggg cgcgtgtcgc ccgtccacgg gccgggattg
      121 cccgtgcccc agtacgtgag cccgagcttc gggtcgaagc tgcccgtgac ccagaccggc
      181 gcgccgcccg tgcgccacga gtcgccgggc cacgtgtcgt tgccgggctc gccgggcgcg
      241 ggcacggtgt aggtcttcca gagttgctcg ccggtgttcg cgtcgagcgc gacgacgaag
      301 ccgcgaatgc cgcgctcgcc gcccgagacg ccgaccatga tcttgccgcg cgccgcgagc
      361 ggcgcgatcg tcatgtagta gccggtgttg tagctctcga ccgctttgtt ccagacgacg
      421 gcgccggtct tcgcgtcgag cgcgacgacg cgcgcatccg acgtcgccgt gtagaccttg
      481 tcgccgtaca gcgcgacgcc gcgattcgtc gggtgcccca tgcggatgtc ggcagggatc
      541 gtatgccggt agcgccacag caagtcgccg gtcgccgcgt cgatcgcgat gacctggttg
      601 aacggcgtcg tgatgaacat gacgccgtcg ttgacgatcg gcggcgactg atggccttcc
      661 gcggtgcccg tcgacatcgt ccacacgggc acgaggttcg cgacgttgcg cgtattgatc
      721 tcgcgcaacg ggctgtagct ccacgagttg tactcgcgca ggaacatgag ccagttgcgc
      781 ggctccggcg actcgagccg cgcatccgtc acggcgctgt agctgcgcgt gctttgcgcg
      841 ccgcacgcga acgcggcgag cgcgatgccg gtggctgctg ccgcgcgagc gtaatggcga
      901 atcatgacgt tctccttatt gtggcggcgc tgcgctgaag ggatcgagcg cctggaatcc
      961 gactcggcct tcgaatgggc cggcgacgac gagcgcgccg tcgacgagct tgatcggcaa
     1021 cgcggcgagc ggcaacggtg cgggtccgcc gacgacgcgc gcgccgtcac cggggtcgaa
     1081 ctccgactcg tggcaagggc acttgaagtg cagcgtgtcg ccgaaccagt cggtcacgtc
     1141 gcagcccgtg tgcgtgcaga cgcccgagta cgcgacgacg ccatcggcgg cgcgggctcg
     1201 agtcgcatca atgagcttgt cgggcggcag ccgcaccacg atgagctggt tcagccgcga
     1261 gccgtcgcgg acgcggcgcg tggcgggctg catcggatag gcgagaatct gcttcgcgcc
     1321 gagcgcgacg tcggccggcg cgatgacctg gcccgcgcga tcgccgtacg cgaacacgag
     1381 cgcatcgccg gcctgcggcg gcgctgcggc gtcgtccaga tcgtcgccgc gcgcggcaac
     1441 cggtgcgcac agcgcgaggc cgcaacaggc cttgagcacg cggcggcgcg gcgtattgcg
     1501 gtaagcgacg gcggtttcgc gccgatccgg catgcgggcc cccctaggac aaaacgcagc
     1561 tcgcagctta tcgtaagcct tgggtctgcg cgctaagggg ggcggatggc ccgcagtgtc
     1621 aagacgtcgg tcgtggggag ctacccgcag cccgactggc tcatcgatcg cgcgcggctc
     1681 acgaagatga tggcgccgcg gattcgcgtc cgcgaagtat ggcgcgttcc gccggatcgc
     1741 ctcgagcaag cgcaggacga ggcgacgctc gaagcgatcc gcgaccagga gcgcgcgggc
     1801 gtcgacatca tcacggacgg cgagattcgc cgcgagagct attcgaacaa gtttgcgaac
     1861 gcgctcgacg gcatcgactt gacgcgcgtc ggcgagatcg tcggccgcac ggggaacagg
     1921 atccccgtgc cgatcgtctc gggcccgatc aggcggcgcg gccccgtcga ggtccgcgac
     1981 gtcgagttcc tgcgcaagaa cacgagcaag acgatcaaga tcacgctgcc cgggccgttc
     2041 acgatggcgc agcaggccgt caacgagcat taccgcgacg acgagagcgt cgcgctcgcg
     2101 tacgccgagg ccgtcaacga ggaggtgcac gacctcttcg cggccggcgc agacgtcgtg
     2161 cagctcgacg agccgtggct gcaagcgcgc gccgaggccg cgcgccggta cggcatcaag
     2221 gcgatcaacc gcgcgctggc cggcgtgcgc ggaacgaccg cgctgcactt gtgtttcggt
     2281 tatgccgcgc tggtcaagga caagccggcg agcggctatt cgtttctcgc cgagctcgag
     2341 gag
//
LOCUS       DASSNN010000712         3979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_121659, whole genome shotgun sequence.
ACCESSION   DASSNN010000712 DASSNN010000000
VERSION     DASSNN010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121659"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFL84_13375"
     CDS             complement(<1..350)
                     /locus_tag="VFL84_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019011286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7609665.1"
                     /translation="MTHSVAHWQTQAAVKDRLMRPLRDLRISVIDRCNYRCPYCMPAE
                     VFGENHKFLPRAHWLTPGEIKRVASVFLQLGVKKLRLTGGEPLLRRDIVEIVAGLAEL
                     HGAEDLAITTNGSRL"
     gene            569..1828
                     /locus_tag="VFL84_13380"
     CDS             569..1828
                     /locus_tag="VFL84_13380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HET7609666.1"
                     /translation="MNRSRLEPLGVPETEYDIEEAASRHGPSALDRWLLKQIVTRLGP
                     ESPVRAALWDAPDVAPRDGAVGVRILDRKALWQLVVNPGLHFGDLYSAGRVQVRGDLL
                     TLLLEAYRFSDKHSTLRGALAHGRKLAPDLSDSKRNIHHHYDIGNDFYKLWLDREALQ
                     YTCAYFADPAMTIEQAQQAKMHHVCRKLQLKAGERVVEAGGGWGGFALFMAKNYGVRV
                     RSFNISKEQVAHSRAWAKELKLDGQVEFVEDDYRNLTGTYDAFVSIGMLEHVGKGNYK
                     DLGALMSRALTKEGRGLVHSIGRDAPALMNAWIDKRIFPGAYPPTLREMMDMFEPNAL
                     SVLDVENIRLHYAKTLEWWLQRYEENVDTVRQMFDETFVRAWRLYLVGSIAAFLGGKL
                     QLFQVLYSRTGAKNVPWTRAHVYRDHP"
     gene            1806..3155
                     /locus_tag="VFL84_13385"
     CDS             1806..3155
                     /locus_tag="VFL84_13385"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7609667.1"
                     /translation="MSTAITPESYAAKAARLTSTWQRGDGPLRLQKSTISNLFRYQPR
                     GAAARRLSLGEFNHVLGIDNAGRTVEVEGLTTYETVVRHCLEHGFLPLVAPELKHITV
                     GGATVGIGIESTCYRYGFVHDGLREADVLLPNGTIVVARPDNEHADLFDALPNSYGTL
                     GYILRAKIALHRAGPYVHLHVEKFRDAAAYLEAMRIATQSPDLQFVEGLFFEDNRYFL
                     MTGRFVDKVQYCDDIVRRSIFYRLVETRRDIYLKTFDYIFRYDPDWFWNVPETGAYNA
                     FRRYAPERWRNSAFYTKYVALKRKALGTVGVSDDRTEPLIQDWEVPWARAEELIRFAI
                     DEVDLDGRPWAAVPIKAPRQPTLYPIKPNELYFNLGCYCQVRRPDGMAPYHYTKILDR
                     KCFDLGGIKMLYSSSFLNETEFDALYNGAAYKKLKAKYDPGGNAQTLYKKVALAATR"
     gene            3292..3741
                     /locus_tag="VFL84_13390"
     CDS             3292..3741
                     /locus_tag="VFL84_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET7609668.1"
                     /translation="MSEHRATIDWQRSSADFTYMTYNRAHDWRFTTVSVPASATKEYR
                     GDETRVNPEEALVASLSSCHMLTFLAVAAKRKLPLDAYRDEAVGVLEKNAEGKLAITR
                     VTLRPQIKWSPGVTVSAADLEKMHHDAHVGCFIANSVKTEITVEPQP"
BASE COUNT          766 a         1313 c         1236 g          664 t
ORIGIN      
        1 aggcgcgagc cgttcgtcgt gatcgcgagg tcctcggcgc catggagctc cgccagaccg
       61 gcgacgatct cgacgatgtc gcgacgcagc agcggctcgc ctccggtcag ccgcagcttc
      121 ttgacgccga gctgcaggaa gacgctcgcg acgcgcttga tctcgcccgg cgtcagccaa
      181 tgcgcacgcg gcaggaactt gtggttctcg ccgaacacct cggccggcat gcaatacgga
      241 cagcggtagt tgcaacggtc gatgaccgag atacgtaggt cgcgcaacgg ccgcatgagc
      301 cggtctttga cggccgcctg tgtctgccaa tgcgctacgg aatgagtcat gttcgtcgga
      361 tagcaaccat ggcagccgct ttgggaaggc tgccgaattt cgtgccgcta tagtaaccta
      421 cgggttggaa atcgaccgcg cccaaacgtc ggcgcggcat tttggcaaac atgccggcgc
      481 cccatcccgc aggggccgaa ctcggcgtga gaccgagcgg caagggcttt gacggcccgc
      541 agtgctggag ttgcagggtg gatcgaccat gaaccgaagt cgtttggagc cgttgggcgt
      601 tccggaaacg gaatacgaca tcgaggaagc tgcttctcgc catgggccga gcgcgctcga
      661 ccgctggctg ctgaagcaga tcgtaacgag gctcggcccg gagtccccgg tgcgcgcggc
      721 cctctgggac gcgcccgacg tcgcgccccg cgatggcgcc gtcggcgtgc ggattctcga
      781 ccgcaaggcg ctctggcagc tcgtcgtgaa tccgggcctt cacttcggcg acctgtacag
      841 cgcgggccgc gtccaagtgc gcggcgacct gttgacgctg ctcctcgagg cctatcgctt
      901 cagcgacaag cattcgaccc tgcgaggggc gctcgcgcac ggccgcaagc tcgcgccgga
      961 cctttccgac tcgaagcgca acatccatca ccactacgac atcggcaacg acttctacaa
     1021 gctctggctc gaccgcgagg cgctgcaata cacgtgcgcc tacttcgcgg atcccgcaat
     1081 gacgatcgag caggctcagc aggcgaagat gcaccacgtc tgccgcaagc ttcagctcaa
     1141 agccggcgag cgcgtcgtcg aagccggcgg cggctggggc ggatttgcgt tgttcatggc
     1201 gaagaactac ggcgtccgcg tgcgcagctt caacatctcg aaggagcagg tcgcacattc
     1261 gcgcgcctgg gccaaggagc tcaagctcga cggccaagtc gagttcgtcg aggacgacta
     1321 tcgcaacctg accggcacgt acgacgcatt cgtctcgatc ggcatgctcg agcacgtcgg
     1381 caagggcaat tacaaggacc tcggcgcgct gatgagccgc gcgctgacca aggaaggccg
     1441 cggcctcgtc cacagcatcg gccgagacgc gccggcgctc atgaacgcgt ggatcgacaa
     1501 acgcatcttc cccggcgcct atcctccgac cctgcgcgag atgatggaca tgttcgagcc
     1561 gaacgcgctc tcggtgctcg acgtcgagaa catccgcttg cactacgcga agacgctcga
     1621 gtggtggctg caacgctacg aggagaacgt cgatacggtc cgccagatgt tcgacgagac
     1681 gttcgtgcgc gcgtggcgcc tctatctcgt cggctcgatc gcagcgttcc tgggcggcaa
     1741 gctgcagttg ttccaggtgc tctactcgcg taccggcgcg aagaacgttc cgtggacgcg
     1801 cgcgcatgtc taccgcgatc acccctgaga gctacgcagc caaggcagca cgtctaacga
     1861 gcacctggca acgcggcgac ggaccgctgc ggcttcagaa gtcgacgatc tcgaacctgt
     1921 tccgctatca gccgcgcggc gccgcggcac gccgcctgtc gctcggcgag ttcaatcacg
     1981 ttctcgggat cgacaacgcc gggcgcacgg tagaggtcga agggctcacg acatacgaga
     2041 ccgtcgtgcg ccactgcctc gagcacgggt tcttgccgct cgttgcgccc gagctcaagc
     2101 acatcaccgt cggcggagcg accgtcggca tcggcatcga gtcgacgtgc taccgctacg
     2161 gcttcgtcca cgacggcctg cgcgaggcgg acgtcctgct gccgaacggc acgatcgtcg
     2221 tagcgcggcc cgacaacgag cacgccgatc tgttcgacgc gctgccgaac tcctacggca
     2281 cgctcggcta catcttgcgc gcgaaaatcg cgctgcaccg ggccgggccg tacgtgcacc
     2341 tgcacgtcga gaaatttcgt gacgccgcgg cgtatctcga agccatgcgc atcgcgacgc
     2401 aatcgccgga tctgcagttc gtggaaggac tgttcttcga ggacaaccgc tatttcctga
     2461 tgacgggccg gttcgtcgac aaagtccagt actgcgacga catcgtgcgc cgcagcatct
     2521 tctaccgtct cgtcgaaacc cggcgcgaca tctacttgaa gaccttcgac tacatcttcc
     2581 gctacgaccc ggactggttc tggaacgtgc ccgaaaccgg ggcttacaac gcgtttcgcc
     2641 ggtatgctcc ggagcgctgg cgcaactcgg cgttctatac caagtacgtt gcgctgaagc
     2701 gcaaggcgct cggcaccgtc ggcgtcagtg acgaccgcac ggagccgctg atccaagact
     2761 gggaagtgcc gtgggcgcgc gccgaggagc tgatccggtt cgcgatcgac gaggtcgatc
     2821 tcgacggccg gccgtgggcc gccgtgccga tcaaagcgcc gcgtcagccg acgctgtatc
     2881 caatcaagcc gaacgagctc tacttcaact tgggctgcta ctgccaggta cggaggcccg
     2941 acggcatggc cccgtatcac tacacgaaga ttctcgacag gaagtgcttc gacctcggcg
     3001 gcatcaagat gctctattcg tcgtcgttct tgaacgagac ggagttcgac gcgctctaca
     3061 acggcgctgc ctacaagaag ctcaaagcga agtacgaccc cggcggcaac gcgcaaacgc
     3121 tgtacaaaaa agtcgccctc gcggcgaccc gttgagctca cccccgaaaa ggggacagat
     3181 ctattttcca gccccgaaaa ggggacagat ctattttcga tccgccgcgg ccggcactcc
     3241 cgccagccct ggcggtttag aatgctcgta aagccctagc tggagcgacc gatgtccgag
     3301 caccgagcca cgatcgactg gcagcgcagc agcgcggatt tcacctacat gacttacaac
     3361 cgcgcgcacg attggcggtt tacgaccgtc agcgtgccgg catcggcgac gaaggagtac
     3421 cgcggcgacg aaacgcgcgt gaaccccgag gaagcgctcg tggcttcatt gtcgtcgtgt
     3481 cacatgctga cgtttctcgc cgtcgccgcg aagcgcaagc tgccgctcga cgcttatcgc
     3541 gacgaggcag ttggcgtgct cgagaagaac gccgaaggga agctcgcgat cacgcgcgtc
     3601 acgctgcgac cgcagatcaa gtggtcgccg ggtgtcacgg tgtccgccgc cgatttggag
     3661 aagatgcacc acgacgcgca cgtcggctgc ttcatcgcga actccgtcaa gacggagatc
     3721 acggtcgagc ctcaacccta gagagccggg ggacagatct attttctgga aaaatagatc
     3781 tgtccccatt ttcagcgcgc gccgaccagc acgcgccgat ctcgccgctc tcgagcagca
     3841 tgaccgtcgg caacgaacgc acgccgtaac gcgccgctgc gagcacggtc ttcgcgaacg
     3901 tcgcgcggtt gacggcgacc gtcaaccgtc gccgttcaaa gcgagcacag cgctcaccac
     3961 ggcagccaaa acggccgtc
//
LOCUS       DASSNN010000713         3425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_251446, whole genome shotgun sequence.
ACCESSION   DASSNN010000713 DASSNN010000000
VERSION     DASSNN010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251446"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VFL84_13395"
     CDS             <1..787
                     /locus_tag="VFL84_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HET7609669.1"
                     /translation="LGVAAQALKTMSRWSWLAVGIGAYIAFTLATFPAGVALRWFAPP
                     GVTFAGVAGTLWSGSAATCTVQGFTVQALRWRLRPASLLLGRVAANVDARIPDGFVRG
                     DVIASASSVRFSDLTGATSLPALQSVLPVRGLRGQASVALESLVLENGWPAVAVGQIK
                     LGKLESLPLIPDGQGAYVPLGDYTVTLVEAPEGELAATFVDNGGPLEVQGTAKLDDRR
                     NYSIDALVEPRAGAPEMLVKGLDIMAAEPDAEGRRRLNLTGSL"
     gene            complement(765..>3425)
                     /locus_tag="VFL84_13400"
     CDS             complement(765..>3425)
                     /locus_tag="VFL84_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET7609670.1"
                     /translation="LQRLVDDEVAAAAALVDARLIAALAPLLRHAAGAANEPLRALLA
                     PWLALDRPPESGAAALAAWRGIAALLLTKQGEWRKRVAKPEGFGPEHAAPRDRLRALL
                     EELAGNEELLAALRTVGTLPDARYTDAQWQSLAALKTVLLRLAAELKVAFAEARAVDF
                     VELALAAQRALGAVEAPSELLLALDRRIQHLLVDEFQDTSLAQRRLLELLTAGWEPDD
                     GRTLFLVGDPMQSIYRFRDADMSLFLLAKQRGVGAVRFRSLTLQRNFRSAPAIVQWVN
                     AAFARVFPAADDVAAGRSAFRESVAARAAGRDQLVHAHACAAVPGAEVESVVAILAAE
                     RQRDPEQSIAVLVQNRRHLEGLRERLRERGLAVHAVEIDSLGEQSIAQDLAGLTRALL
                     HFDDRIAWLAVLRAPWCGLTLADLEALCGDDHASAIWDLLEQPARLARLSEDGKKRAR
                     SIVATLTAAFAMRERLPLARWVERTWTLLGGPACLDDAAEQLSAEQFFALLAAAERGG
                     DLDDPAMLQRALEAVQPQADAPREHGIEIMTMHRAKGLEFDTVVLLGLAREPRPDEPK
                     ALQWLARTAADGHDDLVMVPAAFAGDGELLADFVRRAERERDAAERARLLYVATTRAR
                     ERLHLVWQLAAETEAPPASSLLAHLWPVVCATPRTAEPAASKAGVVAIEPVLRRLAAP
                     AAAAASAAAEPAAPAIRPEFVWAGHAAVHVGTVVHRYLQRIAEQGLERWSAQRVASLR
                     PAFARELELLGVEAAERAGAAERVATALARSLLDVHGRFVLAPHDEARSELRLTVRAG
                     AALEHIRLDRTFVADGKRWIVDFKTSQHEGGALNEFLDSEVARYAPQLERYAVAVAAS
                     DARPVQLALYFPLLGELRAWPSAATASRSG"
BASE COUNT          481 a         1250 c         1246 g          448 t
ORIGIN      
        1 gctcggtgtt gctgcgcagg ccctgaagac catgagccgt tggtcgtggc ttgccgtagg
       61 gataggcgcg tacatcgcgt tcacgctcgc gacgtttccg gccggcgtcg cgctgcgctg
      121 gttcgcaccg cccggagtca cgttcgccgg cgtcgcgggc acgttgtggt cgggcagcgc
      181 ggcgacctgc accgtccaag gcttcacggt ccaggcgttg cgctggcgcc tgcgtcccgc
      241 atcgctgctg ctcggccgcg tggcggcgaa cgtcgacgcg agaattccgg acggcttcgt
      301 cagaggcgac gtcatcgcga gcgcgagcag cgtccgcttc agcgatctga ccggcgcaac
      361 ctcgttgccg gcgctgcaaa gcgtgctgcc cgtgcgcggg ctgcgcggcc aagccagcgt
      421 cgcgctcgag tcgctcgttc tagagaacgg ttggcccgcc gtcgccgtcg gccagatcaa
      481 gctcggcaag ctcgagagct tgccgttgat ccccgacggc caaggcgcgt acgtgcctct
      541 cggcgactac acggtcacgc tcgtcgaggc gcccgaaggc gagctcgcgg cgaccttcgt
      601 cgacaacggc gggccgctcg aggttcaggg cacggcgaag ctcgacgacc ggcgcaacta
      661 ttcgatcgac gcgctcgtcg agccgcgcgc cggcgcgccc gagatgctcg ttaaaggtct
      721 cgacatcatg gccgccgagc ccgatgccga gggccggcgg cgcctcaacc tgaccggctc
      781 gctgtagcgg cggacggcca cgcgcgcagc tcgccgagca acgggaagta caacgcgagc
      841 tgcacgggcc gcgcgtcgct tgccgcgacg gcgacggcat agcgctcgag ctgcggcgca
      901 tagcgcgcga cctccgagtc gagaaactcg ttcagggcac cgccttcgtg ctggctcgtc
      961 ttgaaatcga cgatccaacg cttgccgtcc gcgacgaacg tgcggtcgag gcggatgtgc
     1021 tcgagcgccg cgccggcgcg taccgtgagc ctgagctccg agcgcgcttc gtcgtgcggc
     1081 gccagaacga agcggccatg gacgtccaag agcgagcgcg ccaatgcggt tgcgacgcgc
     1141 tcggcggcgc cggcgcgctc ggccgcctcg acgccgagca gctcgagctc gcgcgcgaac
     1201 gccggccgca gggaggcgac gcgctgcgcg ctccagcgct cgagcccctg ctccgcgatc
     1261 cgctgcaagt agcggtgcac gaccgttccg acgtgcacgg cggcatgacc ggcccagacg
     1321 aactcggggc gtatggccgg ggcggcaggc tccgcggcgg cgctcgccgc ggccgccgcc
     1381 ggcgcggcga gccggcgcag gacgggctcg atcgcgacga cgccggcctt ggatgcggca
     1441 ggctcggcgg tgcgcggcgt cgcgcagacg acgggccaga ggtgcgcgag cagcgagctc
     1501 gccggcggcg cttcagtctc cgccgccagc tgccagacca ggtgcagacg ctcgcgcgcg
     1561 cgcgtcgtcg cgacgtacaa gaggcgcgcg cgctcggccg cgtcgcgctc gcgctcggcg
     1621 cggcgcacga agtccgcgag cagctcgccg tcaccggcga acgcggccgg caccatgacg
     1681 agatcgtcgt gtccgtcggc cgcggtccgc gcgagccact gcagcgcctt cggctcgtcg
     1741 ggccgcggct cgcgcgcgag cccgagcagc acgacggtgt cgaactcgag ccccttggcc
     1801 cggtgcatcg tcatgatctc gatgccgtgc tcgcgcggcg cgtcggcttg cggctgcacg
     1861 gcctcgagcg cgcgctgcag catcgcgggg tcgtcgagat cgccgccgcg ctcggctgcg
     1921 gcgagcagcg cgaagaactg ctccgccgac agttgctccg ccgcatcgtc gaggcacgcc
     1981 gggccgccga gcagcgtcca cgtgcgctcg acccagcgcg cgagcggtaa gcgctcgcgc
     2041 atggcgaacg cggccgtcaa cgtcgcgacg atgctgcgcg ccctcttctt cccgtcctcg
     2101 gagaggcgcg cgagccgggc gggctgctcc agcaaatccc agatcgcgga ggcatgatcg
     2161 tcgccgcaca gcgcttcgag atcggcgagc gtgagcccgc accacggcgc ccgcagcacg
     2221 gcgagccaag cgatgcggtc gtcgaagtgc agcagcgctc gcgtgaggcc cgcgaggtcc
     2281 tgcgcgatcg actgctcgcc caagctgtcg atctcgacgg cgtgcacggc gaggccgcgc
     2341 tcgcgcaacc gctcgcgcaa cccttccaaa tgtctgcggt tctggaccag caccgcgatc
     2401 gactgctccg gatcgcgttg cctctcggcg gccaagatcg cgacgacgct ttcgacctcg
     2461 gcgcccggca cggcggcgca cgcgtgcgcg tgcacgagct ggtcgcggcc ggcggcgcgc
     2521 gccgcgacgc tctcgcgaaa agccgaacgc ccggccgcga cgtcgtccgc ggccggaaac
     2581 acgcgcgcga aagccgcgtt gacccactgc acgatcgcgg gcgcgctgcg gaagttgcgc
     2641 tgcagcgtga ggctgcgaaa ccgaacggcg ccgacgcctc gctgcttcgc gagcaggaac
     2701 agcgacatgt cggcgtcgcg aaagcgatag atcgattgca tcgggtcgcc gacgaggaac
     2761 agcgtgcggc cgtcgtccgg ttcccaaccc gccgtcagca gctcgagcaa ccggcgctgc
     2821 gctagcgacg tgtcctggaa ctcgtcgacg agcagatgct gaatgcggcg gtcgagcgcg
     2881 agcagcagct ccgacggcgc ctcgaccgcg ccgagcgcgc gctgcgccgc gagcgcgagc
     2941 tcgacgaagt cgacggcgcg cgcttcggcg aacgcgactt tgagctcggc cgcgaggcgc
     3001 agcagcacgg tcttgagcgc ggccaggctc tgccactgcg cgtcggtgta gcgcgcatcg
     3061 ggcagcgtgc cgacggttcg cagcgccgcg agcagctcct cgttgccggc cagctcctcg
     3121 agcagcgcgc gcagccggtc gcgcggcgcg gcatgctcgg ggccgaagcc ttcgggtttc
     3181 gcgacgcgtt tgcgccattc gccttgcttc gtcagcaaca gagccgcgat gccgcgccag
     3241 gctgcgagag ccgccgcgcc cgattccggc gggcggtcga gcgcgagcca cggcgcgagc
     3301 agcgcgcgca acggctcgtt cgccgcgccc gcggcatgcc gcagcagcgg cgcgagcgcc
     3361 gcgatcagac gcgcgtcgac gagcgccgcc gcggccgcga cctcgtcgtc gacgagccgc
     3421 tgcag
//
LOCUS       DASSNN010000714         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_861690, whole genome shotgun sequence.
ACCESSION   DASSNN010000714 DASSNN010000000
VERSION     DASSNN010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861690"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..655
                     /locus_tag="VFL84_13405"
     CDS             29..655
                     /locus_tag="VFL84_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609671.1"
                     /translation="MRGEDKPALIVATERYIAAIKQYAACTEGELAAAGGETAPESLR
                     TLLSNRSKSAIAEAAAVAALFAERVAPLNELYLAEFITGEGDDCAPLQRITATTIVDD
                     HAVLFHERNERAYLNVLEAVCPDLERFGHFEIRRNLVGATALEVGPVQTNGLCSHEYI
                     VPYFSLDSVTSRRECALGRFYELDPRQVGRLMEGRSAVRQAAKPPAPE"
     gene            complement(600..1457)
                     /locus_tag="VFL84_13410"
     CDS             complement(600..1457)
                     /locus_tag="VFL84_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HET7609672.1"
                     /translation="MTTSALREFVFGHAKLFVLTGAGCSTGSGIPDYRDADGQWKRGR
                     PIMLQDFVGNERSRQRYWARSLVGFRRMRAARPNGAHRALAALEAQGRITELVTQNVD
                     GLHQAAGSRNVVDLHGRVDVVRCLKCDCRIAREHVQTALVERNPDYASLDALEAPDGD
                     AALDGIDFASFEVPACDACGGMLKPDVVFFGESVPAERVQRAFAALGEADAMLVVGSS
                     LMVYSGFRFVKAMADAGKPIAAVNLGDTRADDLLTLKVAEPCADALSFALTPEREVLP
                     LAAPPSVLP"
     gene            complement(1462..2088)
                     /locus_tag="VFL84_13415"
     CDS             complement(1462..2088)
                     /locus_tag="VFL84_13415"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HET7609673.1"
                     /translation="MSEQRVFALFSTPLYVNNVGDFARPDLRSLEYATAAETGGVYNF
                     RTSVDKNVLHRPEFKAVHDLVMKEVDVYAHGLCGVAHNVHFYVTNSWINVHTRGQAAG
                     QHIHGNSLISGVLYLDVNEMSGDLVFHRDVLSLVPFPPALDFDVDSFNIYNCKSWSYK
                     PKVNDICLFPSTVMHSADPNESDKERWCLAFNVFARGTFGGLHQLTIK"
     gene            complement(2085..>2792)
                     /locus_tag="VFL84_13420"
     CDS             complement(2085..>2792)
                     /locus_tag="VFL84_13420"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7609674.1"
                     /translation="LARDHTVVVTDLRGYGDSGKIAGDAKHALYSKRTMALDQVEVMR
                     HFGFPRFTVFGHDRGARVTHRLALDHPDEVERAAVLDIIPTHYLLSRVDKAFAESAYH
                     WFFFARPAPFPETVIANSLELFVGRGDSTLGTEYRRVFKIPGTLHAMCEDYRAGGSID
                     FEHDGADLDKKVQCPLLVLWGENGSVAGRYDALSIWGERAARLTGKQMPGGHSFQESH
                     PNETAAELRAFLSGTRA"
BASE COUNT          486 a          887 c          941 g          478 t
ORIGIN      
        1 gcccgcgctc gtcgcgattc cggcgcgggt gcgcggagag gacaagccgg ccctcatcgt
       61 cgcgaccgag cgctacatcg ccgcgatcaa gcaatacgca gcctgtaccg aaggcgagct
      121 cgccgccgca ggcggagaga ccgcgccgga gtcgctgcgc accctactga gtaaccgaag
      181 taagtcggcg atcgcggaag ctgcggcggt cgcagctctg ttcgccgagc gtgtggcgcc
      241 tctgaacgag ctgtatctcg cggagttcat cacgggagaa ggggacgatt gcgcaccgct
      301 gcagcgtata acggcgacga ccatcgtcga cgatcacgcc gtcctctttc acgagcgaaa
      361 cgagcgggcg tacctgaacg tgttggaagc ggtgtgcccc gatctggagc gcttcggtca
      421 tttcgagatc cgccgaaacc tcgtcggagc gactgctttg gaggttggac ccgttcagac
      481 gaacgggctt tgtagccacg agtacatcgt tccgtatttc tcgctcgaca gtgtgacgtc
      541 tcgccgcgag tgcgcgctcg gccgctttta cgagctcgat cccaggcaag tgggtcgcct
      601 catggaagga cgctcggcgg tgcggcaagc ggcaaaacct cccgctccgg agtgagggcg
      661 aacgagagag catcggcgca cggttcagcg actttcaacg tcagtagatc gtcggcgcga
      721 gtgtccccaa gattcacggc agcgatcggc ttgccggcgt ccgccattgc tttcacgaat
      781 cggaagccgg agtagaccat cagcgacgag ccgacgacga gcatggcgtc ggcctcgccg
      841 agcgccgcga acgcgcgctg cacgcgctcc gccggcacgc tctcgccgaa gaacacgacg
      901 tcgggcttca gcatgccgcc gcaggcgtcg cacgccggca cttcgaacga agcgaagtcg
      961 attccgtcga gcgccgcgtc gccgtcgggc gcttcgagcg catcgagcga cgcgtagtcg
     1021 gggttgcgct cgaccagggc ggtttgcacg tgctcgcgcg cgatgcggca atcgcacttc
     1081 aggcagcgca cgacgtcgac gcggccgtgc aggtcgacga cgttgcggct gcccgccgcc
     1141 tggtgcaggc cgtcgacgtt ctgcgtgacg agctcggtga ttcggccttg cgcttcgagc
     1201 gctgccagcg cgcgatgcgc gccgttcggc cgcgcggcgc gcatgcgccg gaagccgacg
     1261 aggctgcgtg cccagtaccg ttgccgcgag cgttcgttgc ccacgaagtc ttgcagcatg
     1321 atcggccggc cgcgcttcca ttggccgtcg gcgtcgcggt agtcggggat gccggagccc
     1381 gtgctgcaac ccgcgcccgt cagcacgaac agtttcgcgt ggccgaagac gaactcgcgc
     1441 aatgcggaag tcgtcatcgc actacttgat ggtgagctgg tgcagcccgc cgaacgtgcc
     1501 gcgcgcgaac acgttgaatg ccaagcacca gcgctctttg tcggactcgt tcggatccgc
     1561 cgagtgcatg acggtcgacg gaaacaagca gatgtcgttg accttcggct tgtagctcca
     1621 gctcttgcag ttgtagatgt tgaacgagtc gacgtcgaag tcgagcgcgg gtggaaacgg
     1681 aaccaagctc aagacgtcgc gatgaaagac gaggtcgccg ctcatttcgt tgacgtcgag
     1741 atacagcacg cccgagatca gcgagttgcc gtggatgtgc tggcccgccg cctggccgcg
     1801 cgtgtgaacg ttgatccacg agttcgtgac gtagaaatgg acgttgtgcg cgacgccgca
     1861 gaggccgtgc gcgtagacgt cgacttcctt catgacgagg tcgtgcacgg ccttgaactc
     1921 cggccggtgc agcacgttct tgtcgacgga cgtcctgaag ttgtagacac cccccgtctc
     1981 ggccgccgtc gcgtactcga gcgagcgcag gtcgggccgc gcgaaatcgc cgacgttgtt
     2041 gacgtagagc ggtgtcgaga acagcgcgaa gactcgctgc tcgctcatgc tctggtcccg
     2101 gacaagaatg cgcgcagctc ggcggccgtc tcgttcggat gcgactcctg gaacgaatgg
     2161 ccgcccggca tttgcttgcc cgtgagacgc gccgcgcgct cgccccagat cgaaagcgcg
     2221 tcgtagcgcc cggcgacgga gccgttctcg ccccagagca cgagcagcgg gcattgcact
     2281 ttcttgtcga gatcggcgcc gtcgtgctcg aagtcgatcg agccgccggc gcgatagtcc
     2341 tcgcacatcg catgcagcgt gccgggaatc ttgaacacgc ggcgatactc ggtgccgagc
     2401 gtcgaatcgc cgcggccgac gaagagctcg aggctattcg cgatcacggt ctccggaaac
     2461 ggagcggggc gcgcgaagaa gaaccagtga tacgcgctct cggcgaacgc cttgtcgact
     2521 ctgctcaata gataatgtgt cgggattatg tcgagcacgg cggcgcgctc gacttcgtcc
     2581 ggatgatcga gcgctaggcg atgcgtgaca cgcgcgccgc ggtcgtggcc gaatacggtg
     2641 aagcgcggaa agccgaagtg ccgcatgacc tcgacctggt cgagcgccat cgtgcgcttc
     2701 gaatacagcg cgtgcttcgc gtcgcccgcg atcttgccgc tgtcgccgta gccgcgcaga
     2761 tccgtgacga cgacggtgtg gtcgcgcgcg ag
//
LOCUS       DASSNN010000715         4295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_251952, whole genome shotgun sequence.
ACCESSION   DASSNN010000715 DASSNN010000000
VERSION     DASSNN010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251952"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..850)
                     /gene="bioD"
                     /locus_tag="VFL84_13425"
     CDS             complement(185..850)
                     /gene="bioD"
                     /locus_tag="VFL84_13425"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HET7609675.1"
                     /translation="MATLFVTSSGTGIGKTYVTLRLIAELKAAGRRVRALKPVASGFD
                     AAQPGEGDTGALLRALELEPTAANLDAVSPWRFAAPLSPDMAAARERRAVPFDSLVAH
                     CRTAARQGDVTLIEGIGGVMVPLDDRHTVLDWIAALGAPTLLVVGSYLGTLSHSLTAA
                     AALRGRGVTVAGLVVSESEEQPVAAAETAATLSRFVAPVAVRVLPRAAPVPLLPLVSP
                     YLA"
     gene            complement(851..2023)
                     /locus_tag="VFL84_13430"
     CDS             complement(851..2023)
                     /locus_tag="VFL84_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7609676.1"
                     /translation="MASRSRGARPSLDEIARDKLEALERRHLRRRLVVTAHSSATAAT
                     QSSAELVSFSCNDYLGLSKHPEVVAASIDATRRFGVGAGSSRLVNGNHPLYAELETRL
                     AALKGAEDCVVFGSGYLTNVGVMPALLGRGDLVLLDELCHNSLLTGASLSGARALEFR
                     HNDVGHAAELLAAERSSHRRCLLVTDGVFSMEGDLAPLPALAALAAEHDAWLMTDDAH
                     GLGVVGGGRGSSFAHGAPVSVPLQMGTLSKAVGSYGGYLCASRSVAELVRNRARSFVY
                     STGLPPGTVAAAIRALDLIASDGELVGRPLARARELTTALGLPPATSAIVSLVVGSAA
                     RALAASEALRSAGFLVAAIRPPTVPPGTARLRLTFSAAHTPEQVAALAAAVRPLLA"
     gene            complement(2111..>4295)
                     /gene="lon"
                     /locus_tag="VFL84_13435"
     CDS             complement(2111..>4295)
                     /gene="lon"
                     /locus_tag="VFL84_13435"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET7609677.1"
                     /translation="VDSPGPDDLYRIGTTVSVVRYLKAPNGVHHLICQGEQRFRTLDY
                     LSGLPFLAARFDLIPDQVTTDANIEARAALLKQKAIEAIELLPQVPPELGPALQNIES
                     PGALADMLGGLIDLKPVEKQTILEALKVRDRLDRVIELLTHRVEVLKLSREIGEQTQG
                     KLDERQREYVLREQLKTIKKELGEDETQSELEELRQAIADAKMPEEVEKQAQKELRRL
                     EGMPEASTEHSMVRTYLDWLVSVPWSKSDAETIDIARAREILDADHYGLEKVKRRILE
                     YLAVRKLNAAGRSPILCFVGPPGVGKTSLGQSIARALGLKFVRASLGGVHDEAEIRGH
                     RRTYIGSMPGKIIQSLRKAGTNNPVFMLDEMDKLGVGFHGDPSAALLEVLDPEQNSTF
                     SDHYLDVPFDLSKVFFVATANVLDTIPGPLRDRMEIIELPGYTQDEKLAIATRYLVGR
                     QLKANGLTEDQASITEAALRTVIDGYTREAGVRNLEREIGALLRHAAMKIAEGTAERV
                     DIDAPDVPAILGPRHFENEVKLRTSVPGVATGLAWTPVGGDILFIEATLVPGKGQLIL
                     TGQLGDVMKESAQAAVSLAKGRLDKLGAARSVLETNDLHIHLPAGAIPKDGPSAGVAM
                     FLAVASLLSGRTVRHDIAMTGEISLRGLVLPIGGIKEKALAALRAGIDTVLLPKRNEK
                     DLEEIPEPARKQLKFVFLENIDDALAAAFDPPPGASETARRAAAA"
BASE COUNT          645 a         1477 c         1521 g          652 t
ORIGIN      
        1 gatccggcga gaccgcttcg acggccggcg agaacgacac ggacggcatg gcgctcgacc
       61 gccgcgtcga gatgaacgtc gtcgacggca ccggccgtgt cgcggagctc aacacgaact
      121 gaaagccgca aggttccccc ctagtggccg ggagtcgccg cgaaggaggc ggggctcccg
      181 gccgctatgc caaatagggt gagacgaggg gcagcagcgg caccggcgcg gcgcgcggca
      241 acactcggac cgcaacgggt gcgacgaagc gcgagagcgt cgcggccgtc tccgcagccg
      301 cgacgggctg ctcctctgac tcgctgacga ccaagcccgc gacggtgact ccgcgcccgc
      361 gcagcgccgc ggccgcggtc aggctgtggc tcagcgttcc caagtagctg ccgacgacga
      421 gcagcgtcgg cgcgccgagc gccgcgatcc agtcgagcac ggtgtgccgg tcgtcgagcg
      481 gcaccatgac gccgccgatt ccctcgatca gcgtcacgtc tccttgtctc gcggccgtgc
      541 ggcagtgcgc gacgagcgaa tcgaacggaa cggcgcgccg ctcgcgcgcg gcggccatgt
      601 cgggcgagag cggcgccgcg aatcgccatg ggctcacggc atcgaggttg gcggcggtcg
      661 gctcgagctc gagcgcgcgc agcaacgcgc ccgtgtcgcc ttcgccgggc tgcgcggcgt
      721 cgaagccgga agcgacgggc ttcagtgccc gcacgcgtct tccggcagct ttgagctccg
      781 cgatcaggcg cagcgtcacg tacgtcttgc cgatgccggt gcccgacgac gtgacgaaga
      841 gcgtggccat ctatgcgagc agcggtcgga cggccgcggc gagcgcggcc acttgctccg
      901 gcgtgtgcgc ggccgagaac gtcaagcgca gccgcgcggt gccgggcggc accgtcggcg
      961 ggcggatcgc cgcgacgaga aagccggccg agcgcagcgc ctcgctcgcc gcgagcgcgc
     1021 gcgccgcgct gcccacgacg agcgacacga ttgcgctcgt tgcgggaggc aggccgagcg
     1081 cggtcgtgag ctcgcgcgcg cgcgcgagcg ggcggcccac gagctcgccg tcgctcgcga
     1141 tcaggtcgag cgcgcggatc gcggccgcga cggttccggg cggaaggccc gtcgagtaca
     1201 cgaagctgcg cgcgcgattg cgcacgagct ccgcgacgct gcggctcgcg cagagataac
     1261 cgccgtagct tccgaccgct ttcgacaatg tgcccatttg cagcggcacg ctgaccggcg
     1321 cgccgtgcgc gaagctcgag ccgcggccgc cgccgacgac cccgaggccg tgcgcgtcgt
     1381 cggtcatgag ccaggcgtcg tgctcggccg cgagcgccgc gagagccggc agcggcgcga
     1441 gatcgccttc catgctgaac acgccgtcgg tgaccaacag acagcggcgg tggctcgagc
     1501 gctcggccgc gagcagctcg gccgcgtgac cgacgtcgtt gtggcggaac tcgagcgcgc
     1561 gagcgccgct caaggacgcg cccgtcagca gcgagttgtg gcagagctcg tcgagaagca
     1621 cgaggtcgcc gcggccgagc agcgccggca tgacgccgac gttcgtcagg tagccgctgc
     1681 cgaacacgac gcaatcctcg gcgcctttga gggccgcgag cctggtctcg agctcggcat
     1741 agagcggatg attgccgttc accaaccgcg acgagccggc gccgacgccg aagcggcgcg
     1801 tcgcgtcgat gctcgcggcg acgacttccg ggtgcttcga caagccgagg tagtcgttgc
     1861 acgagaacga gacgagctcg gccgaggatt gcgttgcggc cgtcgcgctc gagtgagcag
     1921 tcacgacgag ccggcggcga aggtgccgcc gctcgagcgc ctcgagcttg tcgcgcgcga
     1981 tctcgtcgag cgaagggcgc gcgccgcgcg aacgtgatgc caactgcgaa gctccggccg
     2041 gtgagcgcgc attctcaccg accgcggcga cggcgggaag ccgtcgacgc ggcctaagcc
     2101 ggcgcagtcc tcaggcggcg gccgctcgcc gcgcggtctc gctcgcgccc ggcggcggat
     2161 cgaacgcggc tgccagcgcg tcgtcgatgt tctcgaggaa cacgaatttc agttgcttgc
     2221 gggccggctc ggggatttcc tcgaggtcct tctcgttgcg cttcggcagc agcaccgtgt
     2281 cgatgccggc gcgcagcgcc gcgagggctt tctccttgat gccgccgatc ggcagcacca
     2341 acccgcgcag gctgatctcg cccgtcatcg cgatatcgtg gcggaccgtg cggcccgaga
     2401 gcagcgaggc gacggcgagg aacatcgcga cgcccgcgct cgggccgtcc ttcgggatcg
     2461 cgccggccgg caagtggatg tgcaggtcgt tggtctcgag gaccgaccga gcggcgccga
     2521 gcttgtcgag gcggcccttc gcgaggctca cggccgcttg cgcgctctcc ttcatgacgt
     2581 cgccgagctg gcccgtcagg atcagctgac ccttgcccgg cacgagcgtg gcttcgatga
     2641 acaagatgtc gccgccgacc ggcgtccagg ctagacccgt cgcgacgccg ggcacgctcg
     2701 tgcgcagctt gacctcgttc tcgaagtgcc gcgggccgag aattgcgggc acgtccggcg
     2761 cgtcgatgtc gacacgctcc gccgtgccct cggcgatctt catcgccgcg tgccgcagca
     2821 gagcgccgat ctcgcgctcg aggttgcgca cgccggcctc gcgcgtgtaa ccgtcgatga
     2881 cggtgcgcag cgcggcctcg gtgatcgagg cctggtcttc ggtcaagccg ttggctttca
     2941 attgccgccc gacgagatag cgcgtcgcaa tcgcgagctt ctcgtcttgc gtgtagccgg
     3001 gcagctcgat gatctccatg cggtcgcgca aggggcccgg gatcgtgtcg agcacgttcg
     3061 cggtcgcgac gaagaacacc ttgctcaagt cgaacggcac gtcgaggtag tgatccgaga
     3121 acgtcgagtt ctgctcggga tcgagcacct cgagcagagc ggcggacgga tcgccgtgaa
     3181 agccgacgcc gagcttgtcc atctcgtcga gcatgaagac ggggttgttc gttcccgcct
     3241 tacgcaagct ctgaatgatc ttgcccggca tcgagccgat ataggtgcgg cgatggccgc
     3301 gaatctcggc ctcgtcgtgc acgccgccca agctcgcgcg cacgaacttg agccccagcg
     3361 ctcgcgcgat gctctggccg agcgacgtct tgcccacgcc gggcggaccg acgaagcaca
     3421 gaatggggct gcggcccgcg gcgttgagct tgcggacggc gagatactcg aggatgcgcc
     3481 gcttgacctt ctcgaggccg tagtggtcgg cgtcgaggat ctcgcgcgcc ctcgcgatgt
     3541 cgatggtctc ggcgtcgctc ttcgaccatg gaaccgagac gagccaatcg aggtacgtgc
     3601 gcaccatcga atgctcggtc gaggcctcgg gcatgccctc gagccgccgc agctccttct
     3661 gcgcctgctt ctcgacttcc tccggcatct tcgcgtccgc gatcgcttgc cgcagctcct
     3721 cgagctcgga ctgcgtctcg tcctcgccga gctccttctt gatcgtcttg agctgctcgc
     3781 gcagcacata ttcgcgctgg cgctcgtcga gcttgccttg cgtctgctcg ccgatctcgc
     3841 gcgagagctt cagcacctcg acgcgatgcg tgaggagctc gatgacgcga tcgagccgat
     3901 cgcgaacctt cagcgcctcg aggatcgtct gcttttcgac cggcttcaga tcgatgaggc
     3961 cgccgagcat gtccgcgagc gcgcccggcg actcgatgtt ctgcagcgcg gggccgagct
     4021 ccggcggcac ttgcggcaac agctcgatcg cctcgatggc cttctgcttg agcaaggccg
     4081 cgcgggcctc gatgttcgcg tccgtcgtga cttgatcggg gatcaagtcg aaacgcgcgg
     4141 cgagaaacgg cagcccgctc agatagtcga gcgtgcgaaa gcgctgctcg ccctggcaga
     4201 tgaggtggtg aacgccgttc ggcgccttca aatagcgcac gacgctgacc gtcgtgccga
     4261 ttcgatagag atcgtccggg cccggcgagt cgacg
//
LOCUS       DASSNN010000716         3298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1633612, whole genome shotgun sequence.
ACCESSION   DASSNN010000716 DASSNN010000000
VERSION     DASSNN010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633612"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1322)
                     /locus_tag="VFL84_13440"
     CDS             complement(36..1322)
                     /locus_tag="VFL84_13440"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET7609678.1"
                     /translation="MSTAKHGVADDLRERMLDLGRAARAAAGVLAQAPADVKRNVLVE
                     AARELRKSRAQVLAANAEDVAAAKASGLSGAMIDRLALDDKRIEATAAGLETVAALDD
                     PVGQVVAEWQRPNGLKIARVRVPLGVIGIIYESRPNVTADAAALCLKSGNAAILRGGS
                     ESARSSAAIHACLARALTKHNLPETAVQMVPTQDREAVGIMLRDMGAFIDVIVPRGGK
                     GLVARVQQDARVPVMSHLEGICHTYVHAAADLEMARRITKNAKLRRTGICGATETILI
                     DRACVATHLQPIVKDLIDAGCEVRGDVRTQSSDKRVRAAVDSDWGQEFLDAIVAVRVV
                     DGLDEALAHIRRYGSAHTDAIVTHDAAAAERFLNELDSAIVMHNASTQFADGGEFGMG
                     AEIGIATGKIHARGPVGAAELTSYKYVVRGTGQIRP"
     gene            complement(1319..2470)
                     /gene="proB"
                     /locus_tag="VFL84_13445"
     CDS             complement(1319..2470)
                     /gene="proB"
                     /locus_tag="VFL84_13445"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET7609679.1"
                     /translation="MSTAAHVTHAERLANARSVVIKIGSALFVDQQTGAMNRAWLEGV
                     CADVADLRRAGKDVVIVSSGAVALGRRELKLARGPALEDKQAAAAAGQIVLAHAYQEI
                     LRGLGLTAAQVLLTLDDSERRERYLNASKTLRTLLKLGAVPVINENDTVATQELRYGD
                     NDRLSARVAQMVSAECLVLLSDVDGLYTADPRRDENARYVEEVGQLTREVLDMAGGPG
                     SSDGSGGMRTKLEAARIAVGAGCRMCIATGRVARPISALLKGGKATWFLPSATPGAAR
                     KQWIAGTLKPRGTLRVDSGAERALAAGRSLLPAGVTQVDGPFGRGDAVSVVAADGREI
                     ARGLTAYPADEAAQIAGRRSGDIQEILGYSGGDEIIHRDDLVLLGTKQS"
     gene            complement(2506..3000)
                     /gene="ilvN"
                     /locus_tag="VFL84_13450"
     CDS             complement(2506..3000)
                     /gene="ilvN"
                     /locus_tag="VFL84_13450"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET7609680.1"
                     /translation="MRHIISMLLQNEAGALARVASVLSSRGYNIESLNVAPTADETVS
                     RLTLVTSGSETVIDQIEKQLGKLVDVVELADMTKSDHIERELALFKLRVTNGAGAKLN
                     PVIEQFGARILSDAGEHYTVELVGGSQDIDRFLKRSSQVADVVTLVRSGAMAVMRGAA
                     LKRG"
     gene            3140..>3298
                     /locus_tag="VFL84_13455"
     CDS             3140..>3298
                     /locus_tag="VFL84_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7609681.1"
                     /translation="MDTLTQPKLARSHPLAGTKMSGADIIIQVLADEGVDTIFGYSGG
                     AILPTYDAV"
BASE COUNT          486 a         1156 c         1099 g          557 t
ORIGIN      
        1 cgcctgcgat gaagccaatc gcggctcggc gagaatcaag gacggatttg gccggtgccg
       61 cggacgacgt atttgtagct cgtgagctcg gcggcgccga ccgggccgcg cgcgtggatc
      121 ttgcccgtcg cgatgccgat ctccgcgccc atgccgaact ccccgccgtc cgcgaactgc
      181 gtcgacgcgt tgtgcatgac gatcgcgctg tcgagctcgt tcaagaagcg ctccgcggcc
      241 gccgcatcgt gcgtgacgat cgcgtccgtg tgcgccgacc cgtagcggcg gatgtgcgcg
      301 agcgcctcgt cgagcccgtc gacgacgcgg accgcgacga tcgcgtccag aaactcctgg
      361 ccccagtccg agtcgaccgc cgcgcgcacg cgcttgtcgc tcgattgcgt gcggacgtcg
      421 ccgcgcacct cgcatcccgc gtcgatgagg tccttgacga tcggctgcag gtgcgtcgcg
      481 acgcacgcgc gatcgatcaa gatcgtttcc gtcgcgccgc agatgcccgt gcgccgcagc
      541 ttcgcgttct tcgtgatgcg gcgcgccatt tcgagatcgg cggcggcgtg cacgtacgtg
      601 tggcagatgc cctcgagatg gctcatgacc ggcacgcgcg catcttgctg cacgcgcgcg
      661 acgaggcctt tgccgccgcg cggcacgatg acgtcgatga aggcgcccat gtcgcgcaac
      721 atgatgccga cggcctcgcg gtcttgcgtc gggaccatct gcacggccgt ctcgggcaaa
      781 ttgtgcttgg tcaacgcacg cgcgagacac gcatgaatcg cggcgctcga gcgcgcgctc
      841 tccgagccgc cgcgcaagat cgccgcgttg ccgctcttca agcacagcgc cgccgcgtcg
      901 gccgtgacgt tcggccggct ctcataaatg atgccgatga cgccgagcgg cacgcgcacg
      961 cgcgcgatct tgaggccgtt cgggcgctgc cactccgcga cgacctggcc gaccgggtcg
     1021 tcgagcgcgg cgaccgtttc gaggccggcg gccgtcgctt cgatgcgttt gtcgtcgagc
     1081 gcgaggcgat cgatcatcgc gccggacagc ccgctggcct tggcagccgc gacatcttcg
     1141 gcgttggccg ccagaacctg ggcgcgcgat ttgcgcagct cgcgcgcggc ttccacgagg
     1201 acgttgcgtt tcacgtcggc cggcgcttgc gccagcacgc cggctgccgc gcgtgccgcg
     1261 cgcccgagat cgagcatgcg ctcgcgcaag tcgtcggcaa caccgtgctt cgccgtgctc
     1321 atgattgctt cgttccgagc agcaccaagt cgtcgcgatg gatgatctca tcgccgccgc
     1381 tgtagcccaa gatctcctgg atgtcgcccg aacggcggcc cgcgatctgc gccgcctcgt
     1441 ccgcggggta cgccgtcaag ccgcgcgcga tctcgcggcc gtcggccgcc acgacgctga
     1501 cggcgtcgcc gcgcccgaac gggccgtcga cttgggtcac gccggccggc agcaggctgc
     1561 ggccggccgc gagcgcgcgc tcggcgcccg agtcgacacg aagcgtcccc ctcggcttca
     1621 acgtgcccgc aatccattgc ttgcgcgcgg cgcccggtgt cgcgctcggc aggaaccacg
     1681 tcgctttgcc gcctttgagc agcgcgctga tcggccgagc gacgcggccc gttgcgatgc
     1741 acatgcggca gcccgcgccg acggcgatgc gcgcggcctc gagcttcgtg cgcatgccgc
     1801 cggagccgtc actcgaaccc ggcccgcctg ccatgtcgag cacttcgcgc gtgagctggc
     1861 cgacctcttc gacgtagcgc gcgttctcgt cgcggcgcgg atcggccgtg tacaagccgt
     1921 cgacgtccga cagcagcacg agacattcgg cgctgaccat ctgcgccacg cgcgcggaga
     1981 ggcgatcgtt gtcgccgtag cgcagctctt gcgtcgcgac cgtgtcgttc tcgttgatga
     2041 ccggaacggc gcccaacttg agcagcgtgc gcagcgtttt gctcgcgttg agatagcgct
     2101 cgcgacgctc gctgtcgtcg agcgtcaaca gcacttgcgc ggccgtgagc cccaagccgc
     2161 gcaagatttc ctggtaagca tgcgcgagca cgatttggcc ggcggccgcg gccgcctgct
     2221 tgtcctcgag cgccgggccg cgcgccagct tgagctcgcg ccgcccgagc gcaacggcgc
     2281 ccgacgagac gatgacgacg tccttgccgg cgcggcgcag atcggcgacg tccgcgcaga
     2341 ccccctcgag ccacgcgcga ttcatcgcgc ccgtctgttg gtcgacgaac agcgccgagc
     2401 cgatcttgat gacgacgctg cgcgcgttgg cgaggcgttc ggcgtgcgtc acgtgcgctg
     2461 cggtgctcat gatgcgtggg ctcgctcgaa gcgagcgatg aagcgctagc ctcgtttgag
     2521 cgcggcgccg cgcatgacgg ccatcgcgcc gctgcgaacc agcgtcacga cgtcggcgac
     2581 ctgcgacgag cgtttcaaga accggtcgat gtcctgactg ccgccgacga gctcgaccgt
     2641 gtagtgctcg cccgcatcgc ttaaaatccg ggcgccgaac tgctcgatga cggggttaag
     2701 cttcgcgccg gcgccgttcg tgacgcgcaa cttgaacaac gcaagctcgc gctcgatgtg
     2761 gtcggacttc gtcatgtcgg cgagctcgac gacgtcgacg agcttgccga gctgcttctc
     2821 gatctggtcg atgacggtct cggaacccga cgtgacgagc gtcagacgcg acacggtttc
     2881 gtcggcggtc ggcgcgacgt tcaagctctc gatgttgtaa ccgcgcgaag agagcacgct
     2941 cgcaactcgc gcgagagcgc ccgcttcgtt ctgcaagagc attgaaatga tatgtcgcat
     3001 cggcgatctg ggacccgcgg cgcgcgcagg gtttgcaatg ttggcgtaga actttagcta
     3061 ttgcaatgca ggtccttttc gtcaaaactc cggtggctta gcggctttga gcccgcactc
     3121 ggcttctcgg cgaccaccca tggacactct gactcagccc aagctcgcac gctcgcaccc
     3181 gctcgcgggc acgaaaatga gcggcgcaga catcatcatc caagtgctgg ctgacgaggg
     3241 tgtagacacg atcttcggct acagcggcgg cgcgatcctg ccgacctacg acgccgtc
//
LOCUS       DASSNN010000717         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1329173, whole genome shotgun sequence.
ACCESSION   DASSNN010000717 DASSNN010000000
VERSION     DASSNN010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329173"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1048)
                     /locus_tag="VFL84_13460"
     CDS             complement(116..1048)
                     /locus_tag="VFL84_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET7609682.1"
                     /translation="MRRFEIRLARPLRTVLVALLVAAASSAPAQAPRPQAQPAAAPAP
                     VPAAVAIARPSAAEVQLARDAFAKFATSTDAATRAVLQKYPGLLEVRPPVPNTAVLPS
                     LAPPFQAKFEANKEVAKQGGGELLLVGDSITDFWRNTDGPFAGKPVLDKHFGQWKVAN
                     FGIAGDTTQGVLYRLQNGEGQGFSPRAIMLMIGTNNTARNTAAEIAEGIGAVVLELQK
                     DFPQAKILLLGVFPRGRPNDPVRATIADINRTIAKLHDGQRVHYLDIGAKFLSPDGTI
                     SADVMSDLLHPTPKGYEIWAQAVKEPLTALMAGR"
     gene            1281..1787
                     /locus_tag="VFL84_13465"
     CDS             1281..1787
                     /locus_tag="VFL84_13465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7609683.1"
                     /translation="MLYRGVAVLGLLAVSPAHAALIEYSFTSTLADYGTGAQENFGVE
                     VGGPANGGFAFDDAAVRTYYSQDLFDHTLNATGDELWIGDAAPHVYSADTWRLDGNAV
                     SGYVVNGMHFLLQEWFCGPLLNADSWGYGGSVKPKSVPEPASLSLLAMGAFGALALRR
                     RKQAGDRK"
     gene            1980..>2161
                     /locus_tag="VFL84_13470"
     CDS             1980..>2161
                     /locus_tag="VFL84_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609684.1"
                     /translation="MFTMRFWDLFGSDGVSCVKLPAAVAFPTDDRLLRRIEEQRRALP
                     ANNMKPLWRSRETASSP"
BASE COUNT          349 a          683 c          745 g          384 t
ORIGIN      
        1 ggtgggcgaa ggcgggcgcc gcgaatggcg cgccggcggc gagcgcgaag aggggggcga
       61 gacgtcgcat ggggcaacct cggatccaaa tcatacgggc atgatagaga gatcgtcagc
      121 ggccggccat cagcgccgtc agcggctcct tcacggcttg cgcccagatc tcgtagccct
      181 tcggcgtcgg gtgcagaaga tcgctcatga cgtcggccga gatcgtgccg tcgggcgaga
      241 ggaacttcgc gccgatgtcg agataatgca cgcgctggcc gtcgtgcagc ttcgcgatcg
      301 tgcggttgat gtcggcgatc gtcgcgcgga ccgggtcgtt cggccggccg cgcgggaaca
      361 cgccgagcag caagatcttg gcttgcggaa aatccttttg cagctcgagc accacggcgc
      421 cgatgccttc ggcgatctcg gccgccgtgt tccgcgccgt gttgttcgtg ccgatcatca
      481 gcatgattgc gcgcgggctg aacccctggc cctcgccgtt ttgcaggcga tagagaacgc
      541 cctgcgtcgt gtcgcccgcg atcccgaagt tcgcgacctt ccactggccg aagtgcttgt
      601 cgagcacggg cttgccggcg aagggaccgt cggtgttgcg ccagaagtcg gtgatcgagt
      661 cgccgacgag cagcagctcg ccgccgcctt gcttcgcgac ctctttgttc gcttcgaatt
      721 tcgcctgaaa cggcggcgcg aggctcggca ggacggccgt gttcggcacg ggcggccgca
      781 cctcgagaag cccgggatac ttctgcaaga cggcgcgcgt cgcggcatcc gtcgacgtcg
      841 cgaatttcgc gaacgcgtcg cgcgcgagct gaacctcggc tgcgctcggg cgcgcgattg
      901 cgacggcggc gggtacgggc gcaggcgcgg ctgcgggctg ggcctgcggt cgcggcgctt
      961 gcgccggcgc gctcgaggcg gccgcaacga gcaacgcaac caataccgtt cgaagcggac
     1021 gagcgagacg aatctcgaaa cgacgcatgg ccggaccccc ctgatccgaa ttcgaccgcg
     1081 tgattctgcc cgtccgccgg gcgaaccgcc agaagcaccg aggttccaaa acctttccaa
     1141 aacttggttt aaccgccgcg cgttttgcag cgcgcttcga ggccgagccg cgcgagatcg
     1201 cgacgggcgc gccggcagcg tcgggcacga tacttgctta gttggaaggg tcatcaggga
     1261 aggcttccag ggaggcttac atgttgtacc gaggagtagc ggttcttgga ctgctagctg
     1321 tttctccggc ccacgcggcg ctcatcgaat actcgttcac gtccacgctc gccgactatg
     1381 gcaccggtgc tcaggaaaac ttcggcgtcg aagtcggggg cccggccaac ggcggattcg
     1441 cgttcgacga cgcggccgtg cggacttact actcacagga tctgttcgac catacgctca
     1501 acgcaaccgg cgacgagctc tggatcggag acgcggcccc gcacgtgtac agcgccgaca
     1561 cgtggcgcct cgacggcaac gccgtcagcg gttatgtcgt caacggcatg catttcttgc
     1621 tgcaggaatg gttctgcggt ccgctcttga acgccgactc gtgggggtac ggcggctcgg
     1681 tcaagccgaa gtccgtgccg gagcccgcat cgctttcgct gctcgcgatg ggcgcgttcg
     1741 gcgcgttggc gctgcgcagg cgcaagcagg ccggcgaccg caagtagctc gttgcaaagt
     1801 ttgggatcgg taagcgcgtc gctcggcttc ctgacgcccc gctcggcttg ccgctcgcac
     1861 gcgtcaacgt gccgacggta gccgaacgcc ttcgggcgtg ctttttgcat tcgataggga
     1921 aggggatttc cacgcaaggt ccagtgcgca tggacgcgcc gttagggagg gtcgtccaca
     1981 tgttcactat gcgtttctgg gacctctttg ggtccgacgg ggtgtcgtgc gtgaagcttc
     2041 cggcggccgt cgcgtttcct accgacgacc ggttgctgcg aaggatcgaa gagcagcggc
     2101 gcgcgttgcc cgcgaacaac atgaaaccgt tgtggaggag ccgcgagacc gcgtcatcgc
     2161 c
//
LOCUS       DASSNN010000718         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_122475, whole genome shotgun sequence.
ACCESSION   DASSNN010000718 DASSNN010000000
VERSION     DASSNN010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122475"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /gene="gatB"
                     /locus_tag="VFL84_13475"
     CDS             complement(<1..445)
                     /gene="gatB"
                     /locus_tag="VFL84_13475"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637102.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HET7609685.1"
                     /translation="MQWEIVVGLEIHAQLATRSKIFSGASTQYGAAPNSQASLVDLGY
                     PGTLPVLNGECVKMAVKFGLATSCEIARRSVFARKNYFYPDLPKGYQISQYELPIVHG
                     GKVEIVLDDGSTKTIRITRAHLEEDAGKSLHEDFVGMSGIDLNRAG"
     gene            complement(451..1911)
                     /gene="gatA"
                     /locus_tag="VFL84_13480"
     CDS             complement(451..1911)
                     /gene="gatA"
                     /locus_tag="VFL84_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET7609686.1"
                     /translation="MHTRTLAQLAGDLRARRVSATELTRHYLSRIERFDAKLNAFITV
                     TAEQALAQAAVADRRLAKGDASPLTGLPVAHKDIFCTSGVKTTCASKMLDNFVAPYDA
                     TVVERLANAGAVMLGKTNMDEFAMGSSNETSFYGPVHNPWDVSLVPGGSSGGSAAAVA
                     ARIAPAATGTDTGGSIRQPAALAGITGLKPTYGRVSRYGMIAFASSLDQAGVLTQTAE
                     DAALLLEAIAGFDERDSTSLDDAVPPYSKLVAEPWDDVTIGVPESFFDDGLDADNAKA
                     VRAALKELTALGAKLKPVELEYIHLSVPAYYVVAPAEASSNLSRFDGVRFGHRAEHAA
                     GADLVTFYMRNRGEGFGAEVKRRILTGTYVLSAGYFDAYYLQAQKARKLISDDFARAF
                     RDVDLVAGPTTPSPAFGIGQKTSNPIEMYLNDIYTIGANLAGLPAISIPCGFVRNLPV
                     GLQLVAPPLNEARLLKVAHHFQKVTDWHQRMPGGYA"
     gene            complement(1968..2252)
                     /gene="gatC"
                     /locus_tag="VFL84_13485"
     CDS             complement(1968..2252)
                     /gene="gatC"
                     /locus_tag="VFL84_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET7609687.1"
                     /translation="MLRPEDLERLCRLARLEITPAEVPDVLAKLSSIVAMVGELTACD
                     TTGVTPMAHPLERGERLRADVVTESDRHELYQRNAPLVERDLYLVPKVIE"
     gene            2386..3429
                     /locus_tag="VFL84_13490"
     CDS             2386..3429
                     /locus_tag="VFL84_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET7609688.1"
                     /translation="MLKSFLGYFSNDLSIDLGTANTLIYVRGRGIVLNEPSVVAIREE
                     PGRGGKVLEAVGIEAKNMLGRTPGHITAIRPMKDGVIADFSVTEKMLQHFIKKAHGSR
                     YFRPSPRVLVCVPYGSTQVERRAIRESAEGAGARKVYLVEEPMAAAIGAGMPVHEARG
                     SMVLDIGGGTSEVAVISLNGIVYASSVRIGGDRFDDAITNYVRRNYGILIGEATAERI
                     KHEIGSAYPGQDVREISVKGRNLSEGVPRSFTLNSNEILEALQEPLQGIVGAVKVALE
                     QTPPELGADVAERGIVLTGGGALLTDIDKLLTEETGLPVVIADEPHTCVARGGGRVLE
                     LLDEYGPSAFSVE"
BASE COUNT          600 a         1158 c         1193 g          631 t
ORIGIN      
        1 tgcccgcgcg gttcaagtcg atgccggaca tcccgacgaa gtcctcgtgc agcgacttgc
       61 cggcgtcctc ttccagatgc gcgcgcgtga tcctgatcgt cttggtgctg ccgtcgtcga
      121 gcacgatctc gactttgccg ccgtgcacga tcggcagctc gtactggctg atctgatagc
      181 ccttcggcaa gtcgggatag aagtaattct tgcgcgcgaa cacggagcgg cgcgcgatct
      241 cgcagctcgt cgcgagcccg aacttgaccg ccatcttcac gcactcgccg ttcagcaccg
      301 gcaacgtgcc gggatagccc aaatcgacca agctcgcttg gctgttcggc gccgcgccgt
      361 actgcgtcga cgcgcccgag aagatcttcg agcgcgtcgc gagctgcgcg tggatctcga
      421 ggccgacgac gatttcccac tgcatggcgc tcacgcgtag ccgcccggca tgcgctgatg
      481 ccagtccgtg accttctgga agtgatgcgc gaccttcagc aagcgcgctt cgttgagcgg
      541 cggcgcgacg agctgcaagc cgaccggcag attgcgcacg aagccgcacg gaatcgagat
      601 cgcgggcaag cccgcgaggt tcgcgccgat cgtgtagatg tcgttcaggt acatctcgat
      661 cgggttactc gtcttctggc cgatcccgaa cgccggcgac ggcgtcgtag gtcccgcgac
      721 gagatcgacg tcgcgaaacg cgcgcgcgaa gtcgtcgctg atgagcttgc gcgccttttg
      781 cgcttgcagg tagtacgcgt cgaagtagcc cgcggagagc acgtacgtgc ccgtcaggat
      841 gcggcgcttg acctcggcgc cgaaaccctc gccgcggttg cgcatgtaga acgtcacgag
      901 gtccgcgccg gcggcgtgct cggcacgatg gccgaagcgc acgccgtcga agcgcgacag
      961 attcgacgac gcttcggccg gcgcgacgac gtagtacgcg ggcaccgaca agtgaatgta
     1021 ctcgagctcg acgggcttca gctttgcgcc gagagcggtg agctctttca gcgccgcgcg
     1081 cacggctttg gcgttgtcgg cgtcgagccc gtcgtcgaag aagctctcgg gcacgccgat
     1141 cgtcacgtcg tcccacggct cggcgacgag cttcgagtag ggcggaacgg cgtcgtcgag
     1201 cgacgtcgag tcgcgctcgt cgaaaccggc gatcgcctcg agcagcagcg ccgcgtcctc
     1261 ggccgtctgc gtcagcacgc cggcctggtc gaggctcgac gcgaacgcga tcatgccgta
     1321 gcgcgaaacg cgcccgtacg tcggcttgag acccgtgatg ccggcgagcg cggcgggctg
     1381 gcgaatcgag ccgcccgtgt cggtgcccgt cgcggccggc gcgatgcgcg cggcgaccgc
     1441 ggcggcagag ccgcccgacg agccgcccgg cacgagcgat acgtcccacg gattgtggac
     1501 cggaccgtag aagctcgtct cgttcgacga gcccatcgcg aactcgtcca tgttcgtctt
     1561 gccgagcatg acggcgccgg cattcgcgag gcgctcgacg acggtcgcgt cgtacggcgc
     1621 gacgaagttg tcgagcatct tcgacgcgca cgtcgtcttg acgccggacg tgcaaaagat
     1681 gtccttgtgc gctacgggta agccggtcag cggcgaggcg tcgcccttcg cgagtcgtcg
     1741 atcggcgaca gctgcctgtg cgagcgcttg ctcggccgtg acggtgatga acgcgttcag
     1801 cttcgcgtcg aagcgctcga tgcggctcaa gtagtgacga gtcagctccg tcgccgagac
     1861 gcgccgtgcc cgaaggtcgc cggccaattg tgcgagagtc cgtgtgtgca tgcggaatga
     1921 ttgccgaagg cttccgcgcc ttgccttaga ggcgcggcgc cgctgatcta ttcgatgact
     1981 ttgggaacga gatatagatc acgttccacg agcggcgcat tgcgttgata tagctcgtgc
     2041 cggtcgctct cggtgacgac gtcggctcgc agccgctcgc cccgctcgag cggatgcgcc
     2101 atcggcgtca cgcccgtcgt atcgcacgcc gtaagctccc cgaccatggc cacgatgctc
     2161 gaaagcttgg ccagcacgtc gggcacttcg gcgggcgtga tctcgagtcg ggcgagccgg
     2221 caaaggcgct ctaagtcttc gggtcgaagc acgggtttct cggcggaaat gagcgggctt
     2281 ggaagtggag tgatcgactt ctctttaata atacatgtcg cttgtggatt ggcctaccca
     2341 ttgttaaagt cgcgccacgt ttccccctag cggccttggg cctcaatgtt aaagagcttt
     2401 ctgggatatt tctccaacga tctatccatc gatctcggca cggctaatac cctgatttat
     2461 gtccgcggac gcggcatcgt cctgaacgag ccgtcggtcg tggcgatccg cgaagagccg
     2521 ggtcgcggcg gcaaggtgtt ggaggccgtc ggcatcgagg cgaagaacat gctcggccgc
     2581 acgccgggcc acatcacggc gatccggccg atgaaagacg gcgtcatcgc cgacttcagc
     2641 gtcaccgaga agatgctgca gcacttcatc aagaaggcgc acggctcgcg ttacttccgg
     2701 ccgagcccgc gcgtgctcgt ctgcgtgccg tatggctcga cgcaagtcga gcgccgcgcg
     2761 attcgcgagt cggccgaagg cgccggcgcg cgcaaagttt atctcgtcga ggagccgatg
     2821 gccgccgcga tcggcgccgg catgcccgtg cacgaggcgc gcggctcgat ggtgctcgac
     2881 atcggcggcg gcacctccga ggttgccgtc atctcgctga acggcatcgt gtacgcatcg
     2941 tcggtgcgca tcggcggtga ccggttcgac gacgcgatca cgaactacgt tcgccgcaac
     3001 tacggcatct tgatcggcga agccacggct gagcgcatca agcacgagat cggctcggca
     3061 tatccgggtc aagacgtgcg cgaaatttcc gtgaagggcc gcaacctctc cgagggcgtg
     3121 ccgcgcagct tcaccctgaa cagcaacgag atcctcgagg cgttgcaaga gcctttgcag
     3181 ggcatcgtcg gtgccgtcaa agtcgcgctc gagcaaacgc cgccggagct cggcgccgac
     3241 gtcgcggagc gcggcatcgt tctgacgggc ggcggcgcgc tcctgaccga catcgacaag
     3301 ttgttgaccg aggaaaccgg attgccggtc gtcatcgccg acgagccgca cacgtgcgtc
     3361 gcccgaggcg gcggccgcgt gctcgagctg ctcgacgaat acggaccgtc ggcattctcg
     3421 gtggagtagc ttcggaccta gcaccagcgc aggaggacgc atgagtgcag caggaagagc
     3481 cagtcccggc ttcggtcgaa gcgggttctc catgggcgct cgcttcctgc tcctcgccgt
     3541 cgcctgcatc gcgctgatga tgctcgatca gcgccagcat ca
//
LOCUS       DASSNN010000719         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_497689, whole genome shotgun sequence.
ACCESSION   DASSNN010000719 DASSNN010000000
VERSION     DASSNN010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497689"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VFL84_13495"
     CDS             complement(<1..1109)
                     /locus_tag="VFL84_13495"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HET7609689.1"
                     /translation="MKRNVLLIATGMIVGIAVLAALRATAWQFREGEPPTLAPLLGRV
                     TPAVVNIAVVGTAAEARNPLLEDPFFRRFFEGPDAPAMPPSVPRQSIGSGVIIDGHAG
                     LVVTNHHVVQGADSIVVTLSDRREFNAMLVGSDAGTDVALLRVMADNPSFADIPLGDS
                     NAVSVGDYVVAIGNPFGLGQSATAGIVSAMGRSGVNIESYEDFIQTDASINPGNSGGA
                     LVDLNGRLLGVNTAILSGSGGNIGIGFAIPSNMVSEVVQQLLEHGNVQRGRIGIAIQD
                     VTPGLAQALDLKTDRGALVTQVEPGSPAEEAGIQPGDVVVAIDDEPIDSSADLRNTVG
                     LVRAGESVAVTVLRNGQRRTVRTTVAADATQTAATT"
     gene            1267..2013
                     /locus_tag="VFL84_13500"
     CDS             1267..2013
                     /locus_tag="VFL84_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HET7609690.1"
                     /translation="MDNRISLHCAALIGLTALGVGCERDTSDRQGSARDESAQSRPGD
                     SSLSRNQPGEPAAGANGDSTIRPPATATNPAAPGSAAAPAAPPRLMARAEIQPLGDHT
                     VRGVIEFEQPPPGSSGPMTIHVMLMGLDPGPHGMHVHTGSDCMAPGMHLNPQDAPHGP
                     ANAASGMRHLGDLGNITADASGMVEEMLRDSLLGSDASFVGRVIVVHEGQDDLSTQPD
                     GSSGAPVGCGLIEPAGEDVISRGPAPDRGV"
     gene            complement(2029..>2700)
                     /locus_tag="VFL84_13505"
     CDS             complement(2029..>2700)
                     /locus_tag="VFL84_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET7609691.1"
                     /translation="SLAAYLASASIAGLAIAFGSQGLVQDVVTGLTLIFSDLIDVGDL
                     VEMGGQTGVVRGVTMRFVELENSLGGTVFVPNRTISSVTNYPRGYVRCFVDVTLLGDA
                     ASRQRMQTAAVRLMSSFAREFPGVSAREPAIVGRQKLDSGKEFLRLKFRVWPNRGGPI
                     ETTFRQELEAELKRENAEYQSWMISIAYEIEARAAARLAFSWLLRRADAARERGAARR
                     GQGSR"
BASE COUNT          424 a          936 c          926 g          414 t
ORIGIN      
        1 gtagtcgctg cggtttgcgt ggcatccgcc gcgaccgtcg tgcgcacggt gcggcgctgg
       61 ccgttacgaa gcaccgtgac ggcgacggac tcgcccgcgc gcacgaggcc gaccgtgttg
      121 cgcaagtcgg ccgagctatc gatcggctcg tcgtcgatcg cgacgacgac gtcgccgggt
      181 tggattccgg cttcctcggc cggagagccc ggctcgacct gcgtaacgag cgcgccgcga
      241 tccgtcttca agtcgagcgc ttgcgcgaga cccggcgtga cgtcctggat cgcgatgccg
      301 atgcggccgc gctgcacgtt gccgtgctcg agcagctgct ggacgacctc gctgaccatg
      361 ttgctgggga tcgcaaaacc gatgccgatg ttgccgcccg agccggacag tatcgccgtg
      421 ttcacgccga gcagccggcc gttgaggtcg acgagcgcgc cgcccgagtt gccgggattg
      481 atcgacgcgt cggtctggat gaaatcctcg tagctctcga tgttgacccc gctgcgcccc
      541 atcgcgctca cgatgccggc cgtcgcgctc tgaccgaggc cgaacgggtt gccaatcgcg
      601 accacgtagt cgccgaccga caccgcgttc gagtccccga gcgggatgtc ggcgaacgac
      661 ggattgtccg ccatgacgcg cagcagcgcg acgtccgtgc ccgcgtcgct gccgacgagc
      721 atcgcgttga actcgcgccg gtcgctcagc gtcacgacga tgctgtcggc gccctgcacg
      781 acgtgatggt tcgtcacgac caatcccgcg tgaccgtcga tgatgacgcc cgagccgatg
      841 ctctggcgcg gcacgctcgg cggcatggca ggggcgtcgg gaccttcgaa gaaccggcgg
      901 aagaagggat cctcgagcag cggattgcgc gcttccgccg ctgtgccgac gacggcgatg
      961 ttgacgaccg cgggtgtgac gcgcccgagc agcggcgcca acgtcggcgg ctcgccctct
     1021 cggaactgcc aggcggtcgc gcgcagcgcc gcgagcacgg cgatgcccac gatcattccg
     1081 gtcgcgatga gtagtacgtt gcgtttcatg ctcgattcct ctccgcggcc acgccggacg
     1141 gcaatttcat gggggctcct tgcggtgtgg agcaagttct atgccacgcg catttcgccg
     1201 cggcgggcgc cgcgaccgtt tggcaccaat cttgctgctg accggcacac gttcgaggag
     1261 agagccatgg acaacagaat ttccttgcat tgcgcggcgt tgatcggctt gaccgcgctc
     1321 ggcgtgggct gcgaacgcga cacgtcggac cgccaaggct cggcgcgcga cgaatcggcg
     1381 cagagccgcc ccggcgacag ctcgctgtcg cgcaatcagc ccggcgagcc cgcggccggc
     1441 gcgaatggcg acagcacgat tcggccgccg gctaccgcga cgaatccggc cgcgcccggc
     1501 agcgcggcgg cgccggctgc gccgcctcgc ctgatggccc gcgcggagat ccagccgcta
     1561 ggcgatcaca ccgtgcgcgg cgtcatcgag ttcgagcagc cgccgccggg cagcagcggt
     1621 cccatgacca ttcacgtgat gctgatgggg ctcgacccgg gcccgcacgg catgcacgtg
     1681 cacacgggca gcgactgcat ggcgccgggc atgcatctga atccgcaaga tgcgccgcat
     1741 ggaccggcca acgcggcttc gggcatgcgc catctcggcg acctcggcaa tatcacggcg
     1801 gacgcgtcgg gcatggtcga ggaaatgctg cgcgactcgt tgctcggcag cgacgcgagc
     1861 ttcgtcggca gggtcatcgt cgtccacgag ggacaggacg atctttcgac gcagccggac
     1921 ggcagctcgg gcgcgcccgt cggctgcggg ctcatcgagc cggccggcga ggacgtcatc
     1981 agccgcggcc cggctcccga ccgcggcgtt tgaggagcgc gcatcgcgtc aacgactgcc
     2041 ctgtccgcgg cgagccgctc cgcgctcgcg cgccgcgtcg gcacgccgaa gcagccacga
     2101 gaacgcgagg cgcgccgccg cgcgcgcctc gatctcgtag gctatcgaga tcatccacga
     2161 ctgatattcg gcgttctctc gcttgagctc ggcctcgagc tcttgccgaa acgtcgtttc
     2221 gatcgggccg ccgcgattgg gccagacacg aaacttcaac cgcaggaatt ccttgcccga
     2281 gtcgagcttc tggcggccga caatggccgg ctcgcgcgcc gatacgccgg gaaactcgcg
     2341 cgcgaacgag ctcatgagcc gcaccgcggc agtctgcatg cgttggcgcg acgcagcgtc
     2401 gccgagcagc gtgacgtcga cgaagcagcg gacatagccg cgcggatagt tcgtgacgct
     2461 gctgatggtc ctgttcggca cgaataccgt tccgccgagc gagttctcga gctcgacgaa
     2521 ccgcatcgtg acgccgcgga cgacgccggt ctgccccccc atttcgacga ggtcgccgac
     2581 gtcgatcaag tccgagaaga tcaacgtgag ccccgtgacg acgtcttgca cgaggccctg
     2641 ggagccgaac gcgatcgcga gcccggctat cgaggcgctc gcgaggtagg ccgccagcga
//
LOCUS       DASSNN010000720         4115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1634051, whole genome shotgun sequence.
ACCESSION   DASSNN010000720 DASSNN010000000
VERSION     DASSNN010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634051"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /gene="tuf"
                     /locus_tag="VFL84_13510"
     CDS             <1..181
                     /gene="tuf"
                     /locus_tag="VFL84_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012519826.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET7609692.1"
                     /translation="TDVTGAVELPANTEMVMPGDNIQMKVELISPIAMEEGLRFAIRE
                     GGRTVGAGVVAKILE"
     gene            200..511
                     /gene="rpsJ"
                     /locus_tag="VFL84_13515"
     CDS             200..511
                     /gene="rpsJ"
                     /locus_tag="VFL84_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET7609693.1"
                     /translation="MANQNIRIRLKAFDHRLIDKSAREIVETAKRTGATVRGPVPLPT
                     KIERYTILISPHADKDARDQYELLTHKRLMDIVDPTDKTVDALMKLELPAGVDVQIKL
                     N"
     gene            786..1457
                     /gene="rplC"
                     /locus_tag="VFL84_13520"
     CDS             786..1457
                     /gene="rplC"
                     /locus_tag="VFL84_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET7609694.1"
                     /translation="MSVGLVGRKCGMTRIFRENGDSVPVTVIEALPNRVTTVKTAERD
                     GYSAVQVTVGAAPKAHRTTKAEAGHFANAKVDPGRGLYEFRLGEGEGAELKAGAELKV
                     DLFKVGQFVDVSGTTKGKGYTGVIKRHNFSHQYMSHGNSLSHRAPGAIGQRQTPGRVF
                     PGKRMAGRLGADKVTKINLEVVKIDAERNLLLVKGAIPGSENGQVVVRPAVKAAAKAA
                     GKSGN"
     gene            1478..2083
                     /gene="rplD"
                     /locus_tag="VFL84_13525"
     CDS             1478..2083
                     /gene="rplD"
                     /locus_tag="VFL84_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015464030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET7609695.1"
                     /translation="MKVKVKGGSGTLELADATFGAAFNGPLVHQVVTAYLAGARAGTK
                     AQKNRSDVSGSGKKPWKQKGTGRARVGSIRSPIWVGGGRTFAARPRSFEQKVNRKMYR
                     GAMRSVLSELLRQERLIVTPELQVSEAKTKALRGKLEKLGFTTGLVIVEAFDMKLWLA
                     ARNLPGVDVLEAQLIDPVSLVGASHVVVTAAALKIIEERVK"
     gene            2080..2391
                     /gene="rplW"
                     /locus_tag="VFL84_13530"
     CDS             2080..2391
                     /gene="rplW"
                     /locus_tag="VFL84_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET7609696.1"
                     /translation="MSSSADRERLMQVLLGPHVSEKATSVAESGNQVVFKVRNDATKT
                     DVKKAVELLFEVKVERVSVVRIPAKQKRFGQRPGARASWKKAYVRLAPGQDINFMSAE
                     A"
     gene            2394..3224
                     /gene="rplB"
                     /locus_tag="VFL84_13535"
     CDS             2394..3224
                     /gene="rplB"
                     /locus_tag="VFL84_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET7609697.1"
                     /translation="MALRKLKPTSAGQRFVVRVVKEGLHKGDPYEPLLEPFRKQGGRN
                     NQGRITTRHQGGGHKRYYRVIDFKRDKDGVAGKIERLEYDPNRTAYLALVLYADGERR
                     YILAPKRAQVGDPIISGADAPIKPGSALPLKNIPIGSLVHCIEMKPGKGAQLARSAGA
                     SVQLVAREGEYVTVRLRSGEMRKVHVNCRATVGEVGNDEHNLRKIGKAGAQRWRGIRP
                     TVRGVAQNPVDHPHGGGEGKSGQGNPHPVTPWGQKTKGYKTRRNKRTRSMIVRDRRQK
                     "
     gene            3268..3531
                     /gene="rpsS"
                     /locus_tag="VFL84_13540"
                     /pseudo
     CDS             3268..3531
                     /gene="rpsS"
                     /locus_tag="VFL84_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486366.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
     gene            3652..3984
                     /gene="rplV"
                     /locus_tag="VFL84_13545"
     CDS             3652..3984
                     /gene="rplV"
                     /locus_tag="VFL84_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET7609698.1"
                     /translation="MQVVAKLRYARISPQKCRLVADQIRGQSVSNALKTLAFSRKKAA
                     RMMHKVLESAIANAEHNHGADIDELKVSNVLVDAAPFFARYAARAKGRGDRILKRNSH
                     ITVRVGDE"
BASE COUNT          841 a         1274 c         1393 g          607 t
ORIGIN      
        1 gacggacgtg acgggcgcgg tggagctgcc ggcgaacacc gagatggtga tgccggggga
       61 caacattcag atgaaggtcg agctgatctc gccgatcgcg atggaagagg gcctaaggtt
      121 cgcgatccgc gaaggcggcc gcaccgtcgg cgccggcgtc gtcgccaaga tcctcgaata
      181 aggaatagca gagaaaatca tggcgaatca aaacattaga atcaggctca aggcgttcga
      241 tcaccggctc atcgacaaat cggcgcgcga gatcgtcgag acggccaaac ggaccggcgc
      301 gacggtgcgg ggccccgtgc cgctgccgac gaagatcgag cggtacacga tcctgatctc
      361 gccgcatgcg gataaggacg cgcgcgacca gtacgagctc ctgacccaca agcggctcat
      421 ggatatcgtt gatccgacgg acaaaaccgt cgatgcgctg atgaagctcg agctgccggc
      481 cggcgtggac gtgcagatca agctgaatta atggggaccg atttagggac gaggggggac
      541 ggatttgaaa tccgtcccca cacgttgtac tagcgaattg caggtggtta taatgccggg
      601 cttttcagca gcccggaccg tttccagcgg gttgttcagc ttgagagtag ccgtccaagt
      661 tcctggacgg ccgccaaatt tcgacgccgc tttttgcgcg gagtcatggg tgccaaggcg
      721 cgcttagtcg cagccctggc tgaagcgccg gccggtgtag caggcgcgga tagagaggaa
      781 gacaaatgtc agtcggtcta gtgggccgga aatgcggcat gacccgtatt ttccgggaaa
      841 acggcgactc cgttccggtc accgtcatcg aagcgctgcc gaatcgcgtc acgaccgtga
      901 agacggccga gcgcgacggc tatagcgccg tgcaagtcac ggtcggtgca gcgccgaagg
      961 cgcaccgcac cacgaaggcc gaggcaggcc atttcgcgaa cgcgaaagtc gacccgggcc
     1021 gcggtctcta cgagttccgc ttgggcgaag gcgaaggggc cgagcttaaa gccggcgccg
     1081 agctcaaggt cgacctcttc aaggtggggc agttcgtcga cgtcagcggc acgacgaagg
     1141 gcaagggtta cacgggcgtc atcaagcgcc acaacttctc ccatcaatac atgtcgcacg
     1201 gcaactcgct ctcgcaccgt gcaccgggcg ccatcggcca gcggcaaacg ccgggccgtg
     1261 ttttcccggg caagcgcatg gcggggcggc tcggcgccga caaggtcacg aagatcaatc
     1321 tcgaggtcgt caagatcgac gccgagcgca acttgctgct cgtcaaaggc gcgattccgg
     1381 gcagcgagaa cggccaagtc gtagtgcgtc cggccgtcaa agcggctgcc aaagcggccg
     1441 ggaagtcggg caactaatac acgcgcgaga tcgggcgatg aaagtcaaag ttaaaggtgg
     1501 tagtggcacg ctcgagctcg cggatgcgac gttcggcgcc gcgttcaacg ggccgctcgt
     1561 gcatcaggtc gtcacggcct atctcgccgg cgctcgcgcg ggaacgaagg cgcagaagaa
     1621 tcgctccgac gtcagcggca gcggcaagaa gccgtggaag cagaagggca cggggcgagc
     1681 ccgtgtcggc tcgatccgca gcccgatctg ggtcggcggc ggccgcacgt tcgcggcgcg
     1741 cccgcgcagc ttcgagcaga aggtcaaccg caagatgtac cgcggcgcga tgcgctcggt
     1801 gctgtcggag ctgctgcgtc aggagcggct catcgtgacg cccgagctcc aagtctcgga
     1861 ggcgaagacg aaggcgctgc gcggcaagct cgagaagctc ggcttcacaa cggggctcgt
     1921 catcgtcgaa gcgttcgata tgaagctctg gctcgccgcg cggaacttgc ccggagtcga
     1981 cgtgctcgaa gcgcagctca tcgatccagt gagcttggtc ggcgccagcc acgtcgtcgt
     2041 cacggccgcg gcactgaaaa tcatcgagga gcgcgtgaaa tgagctcgag cgccgatcgc
     2101 gaacggctga tgcaggtatt gctcgggccg cacgtctccg agaaggcgac gagcgtcgcc
     2161 gaatccggta accaagtcgt gttcaaggtt cgcaacgacg cgacgaagac cgacgtcaag
     2221 aaggccgtcg agctcctgtt cgaggtcaag gtcgagcgcg tgtccgtcgt gcgcatcccg
     2281 gcgaagcaga agcgcttcgg ccagcgcccc ggcgcgaggg cttcgtggaa gaaggcctac
     2341 gtgcgcttgg cgccgggcca ggacatcaac ttcatgagcg ccgaggctta atcgtggcgt
     2401 tacgcaagct caaaccgaca tcggccggac agcgcttcgt cgtccgcgtc gtcaaagaag
     2461 gattgcacaa gggcgatccg tacgagccgt tgctcgagcc gttccgcaag cagggtggcc
     2521 gcaacaacca gggccgcatc acgacgcggc accagggcgg cgggcacaag cggtactacc
     2581 gcgtcatcga cttcaagcgc gacaaggacg gcgtcgccgg caagatcgag cgcctcgaat
     2641 acgatccgaa ccgcacggcg tatctcgcgc tcgtgctgta tgcggacggc gagcgccgct
     2701 acatcctcgc gccgaagcgc gcgcaggtcg gcgacccgat catctcgggg gcggacgcgc
     2761 cgatcaaacc cggcagtgcg ctgccgctga agaacattcc gatcggctcg ctcgtccatt
     2821 gcatcgaaat gaagcccggc aagggcgcgc agctggctcg cagcgccggt gcctccgtgc
     2881 agctcgtcgc tcgcgagggc gagtacgtga cggtgcgttt gcgctcgggc gagatgagga
     2941 aagtgcacgt gaactgccgt gccaccgtcg gtgaagtcgg caacgacgag cacaacttgc
     3001 gcaagatcgg caaggccggc gcgcagcgct ggcgcggcat tcggccgacc gtgcgcggcg
     3061 tggcgcagaa ccccgtcgac catccgcacg gcggcggcga aggcaagtcc gggcaaggca
     3121 atccgcaccc ggtcacgccg tggggccaga agacgaaggg ttacaagacc cgccgcaaca
     3181 agcggacgcg ctcgatgatc gtgcgcgatc gccggcagaa atagctagag agacccgaac
     3241 cagagagccc cgaattagag aacagatatg tcacgttcga tcaagaaagg cccgttcgtc
     3301 gacgtccacc ttctagacaa ggtggcgaag gccgctgcca cgaacgatcg ccggccgatc
     3361 aagacctggt cgcgccgctc gatgatcgtg cctgacatgg tcgggctcac gatcgcgatc
     3421 cacaacggcc gtcagcacgt gcccgtgctc gtctccgaga acatggtcgg ccacaagctc
     3481 ggcgagttcg cggcgacgcg cacgttcaag ggccactcgg gcgaccgcaa ggtcgcggct
     3541 acggcgggcg ctcccggtgg cggtggcgca ccaggcgctc ccggcgcgcc ggccggcggt
     3601 ggcggcggcg gcaagggcgg cggcgagaag ggcggaaagg agtaagggcc aatgcaagtt
     3661 gtcgccaagc ttcgttacgc gcgcatctcg ccgcagaagt gccggctcgt cgcggatcag
     3721 atccgcggcc agtcggtctc gaacgctctg aagactctcg cgttcagccg caagaaggcc
     3781 gcacgcatga tgcacaaggt gctcgagtcg gcgatcgcca acgccgagca caaccacggt
     3841 gccgacatcg acgagctcaa ggtctcgaac gtgctcgtcg acgcagcgcc attcttcgcg
     3901 cgctacgccg cccgggccaa gggccgcggc gaccgcatct tgaagcgcaa cagccacatc
     3961 accgtccgag taggcgacga gtagcgtatg ggtcaaaagg ttcatccggt cggcattcgc
     4021 ctcggcatca cgaaagagtg gtcgtcgcgg tggtacgcga attcacagac gttccccggc
     4081 tacgtcgagc aggatcaccg catccgcgag ttcct
//
LOCUS       DASSNN010000721         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_862313, whole genome shotgun sequence.
ACCESSION   DASSNN010000721 DASSNN010000000
VERSION     DASSNN010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862313"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFL84_13550"
     CDS             complement(<1..222)
                     /locus_tag="VFL84_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET7609699.1"
                     /translation="MTESIDKPPFRAEHVGSLLRPRSLLELRSKVADGAASASSLRAH
                     EDSCIRELVQLQEELGLRSITDGEFRRNTF"
     gene            complement(560..1357)
                     /locus_tag="VFL84_13555"
     CDS             complement(560..1357)
                     /locus_tag="VFL84_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609700.1"
                     /translation="MKSVLLAAAVLALSIGFADPSFAQRRNAKLPPAGPSPRNAAGRV
                     LLGGAPNDPKGVWTPVFGIGKHLKDLNLIPFQKWARAQYDSHLVSQMEPHTRCKPSGA
                     SREFQTPYGVEIDEFPQLGRIYIFDIGGPHTFRTVYTDGRSHPKDFDPTYYGHSIGWW
                     EGDTFVIDTVGYNEGFWPDRRGLPHTSRLHTVERLTRLDSRTMKYEITFDDPGAYTAP
                     WTTSFNLRWEDGTELYEYICQDANEAGQLLVGVNGTKSVDRTSRIAP"
     gene            complement(1582..2010)
                     /locus_tag="VFL84_13560"
     CDS             complement(1582..2010)
                     /locus_tag="VFL84_13560"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609701.1"
                     /translation="MRSVTSSLVAAAAVLFVSGQAMAHHSFAAEFNADQKVTLTGIVT
                     KVQWTNPHVWFYVNVKDKDTGEVKNYGMEMGAPHLLQRRGWRRETLKIGTEVTVNGFL
                     ARNGSNKVSARSVTLTATGSRPGQTLDAASSKDNNQTAVN"
     gene            complement(2089..>2872)
                     /locus_tag="VFL84_13565"
     CDS             complement(2089..>2872)
                     /locus_tag="VFL84_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609702.1"
                     /translation="DKVTMAGSGRPLFVVKNTAPIPPEQPRPTPRWSDGVPILGSQDA
                     SGGYWAYPTKTVLVQDGVNVPMDRDGLLQNLSDAPKVAPLQPWALALYENRQRRHLRD
                     DPMYLKCEPPGGVRQFQSNLGVSFVEDRARQRIFVLMGSGDRNYRIIYLDGRKPVGQV
                     GGDDNNPLFYGRSVGKWEGDTLVIQTTDFNERFWFTNGGLPHTDMLQLTERFSRPNYD
                     TLHYEVTIDDPGAYTKPWKSSWDLRWVGGEDLPNYFCQDNRP"
BASE COUNT          479 a          937 c          916 g          540 t
ORIGIN      
        1 gaaagtgttg cggcggaact cgccgtccgt aatggagcgc aagccgagct cttcctgcag
       61 ttgcacgagc tcgcgaatgc acgaatcctc gtgcgcccgc agcgagctcg cgctcgccgc
      121 gccgtcggct accttcgagc gcagctctag aaggctgcgc ggacgcagca agctgcccac
      181 gtgctcggcg cgaaagggag gcttgtcgat actctcggtc atgcttgact ctccttgttc
      241 gaacggcttg gtgctcgagc gccgctcatg tcctgcgcga tccatgcgaa cgccgagcga
      301 gatgcgggcg ccgggtttcg acggctgcgc tacgatcggc agcgaccgcg aatggcgcgg
      361 cgagatgccg cgccacgcag cgcatcatag acgagtaatc gaatgaagtg ctgcgccgtc
      421 tcatgatcgg gagggcagcg cgcgctcggc tgttcttgcc gctcgcgagt gcggagacgc
      481 cgccgagcga cgcgacggcg cgatgacgag cgggccgggg tgacgcggcg gctcaatcgc
      541 gagccgccgc ggatttcgct cagggcgcta tgcggctcgt gcggtcgacc gacttcgtgc
      601 cgttgacgcc gaccagcagc tggcctgctt cgttcgcgtc ctggcagatg tactcgtaga
      661 gctcggtgcc gtcttcccag cgcagattga agctcgtggt ccacggcgcc gtataggcgc
      721 cgggatcgtc gaacgtgatt tcgtatttca tggtgcgcga gtcgaggcgc gtaaggcgtt
      781 ccacggtgtg caggcggctc gtatggggaa ggccgcgccg atcgggccag aagccttcgt
      841 tatagccgac cgtatcgatg acgaacgtgt cgccttccca ccagccgatc gaatggccgt
      901 agtaggtcgg gtcgaagtcc ttgggatgag agcggccgtc cgtgtagacg gtcctgaaag
      961 tgtgcggccc gccgatatcg aaaatataga tgcggccgag ctgcggaaac tcgtcgattt
     1021 cgaccccgta cggcgtctga aactcgcgcg aagcacccga aggcttgcag cgcgtatgcg
     1081 gttccatctg cgatacgaga tgcgagtcgt actgagcccg agcccacttc tggaaaggaa
     1141 tgaggttgag gtccttgaga tgcttgccga tgccgaatac gggcgtccag acgcccttcg
     1201 gatcgttggg cgcgccgccg agcagcactc tgccggccgc attcctcggt gagggccccg
     1261 caggtggcag cttggcgtta cgtcgctgtg cgaacgacgg gtcggcgaag ccgatcgaga
     1321 gcgccagaac ggcggccgca agcaaaaccg atttcattgc aaatctcccc ccagcttcgt
     1381 cccggcgttt ttttgtgcgg gacgaagatc gctgcgcagg tctcgagtcc ggggctcgac
     1441 acgcgagccc cgcccaggca aaaaacggcc gcgtctaagg cgccgcctcg ctaagccacg
     1501 actagcgagg cgccgctcct tggtttcggc caaacctccg caggcggcaa gcccgcgcaa
     1561 ataccccggc ggcgtctgcg atcagttgac cgccgtctgg ttgttgtcct tgctcgaagc
     1621 ggcatcgagc gtctgccccg gccggctgcc cgtggccgtc aaggtcacgc tgcgtgcgct
     1681 caccttgttg ctgccgttgc gcgccaggaa gccgttcacc gtcacttccg tgccgatctt
     1741 cagcgtctcc cgccgccagc cgcgccgctg cagcagatgc ggggcgccca tctccatgcc
     1801 gtagttcttc acttcgcccg tgtctttgtc tttcacgttt acgtaaaacc acacgtgcgg
     1861 attcgtccac tgcaccttcg tgacgatgcc cgtcagcgtg accttctgat ccgcgttgaa
     1921 ctccgcagca aacgaatgat gcgccatcgc ctgaccactg acgaacagca cggccgccgc
     1981 ggcaacgagg ctgctcgtta cactacgcat tttcatacct tcctccatca actctcacac
     2041 tgtcactcca ggtgtttcga cgcctcggcc cgcggccggc gccccgccct agggccggtt
     2101 gtcctggcaa aagtaattcg gtaaatcctc gcctccgacc cagcgcaagt cccaactcga
     2161 cttccacggc ttcgtatatg cgccaggatc gtcgatcgtg acctcgtagt gcaaggtatc
     2221 gtaattcgga cgcgagaaac gctccgtgag ctgcagcata tccgtatgcg gcaggccgcc
     2281 gttcgtgaac cagaagcgct cgttgaaatc ggtggtctgg atcacgagcg tgtccccttc
     2341 ccacttgccg accgagcgcc cgtagaacag cgggttgttg tcgtcgccgc ccacctggcc
     2401 gaccggcttg cggccgtcga gatagatgat gcggtaattg cggtcgccgc tgcccatgag
     2461 cacgaatatt cgttgccgcg cgcgatcctc gacgaacgac acgccgaggt tcgactggaa
     2521 ctgccgcacc ccgcccgggg gctcgcactt gagatacatg gggtcgtcgc gcaagtgccg
     2581 tcgctgcctg ttctcgtaga gcgcgagcgc ccacggttgg agcggcgcga ccttgggtgc
     2641 gtcgctgagg ttctggagca ggccgtcgcg gtccatggga acgttcacgc cgtcctgcac
     2701 gagcaccgtc ttcgtcggat acgcccaata gccgccgctc gcgtcctgag agccgagaat
     2761 cggcacgccg tcggaccagc gcggggtcgg gcgaggctgc tcgggcggga tcggcgcggt
     2821 gttcttcact acgaagaggg gccgcccgct gccggccatc gtcaccttgt cg
//
LOCUS       DASSNN010000722         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_626977, whole genome shotgun sequence.
ACCESSION   DASSNN010000722 DASSNN010000000
VERSION     DASSNN010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626977"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1108)
                     /locus_tag="VFL84_13570"
     CDS             complement(74..1108)
                     /locus_tag="VFL84_13570"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609703.1"
                     /translation="MHALARGLIALAALPSVAAAHHSFFGRFDTQKLVEVEGEVTEVL
                     WRNPHAYFGVRVDGVDWDVETSSLTVLKRMGIDEGTIEVGDRIRLAANPPLGEKKEMY
                     ARHVLLADGRELLMNVGLTPRWTKRGATVGDDSLLMAREGDGSRPDLGIFRVWSHTRA
                     IARLFPEVTDPTVDINAYPLTKAARAQLQKFDRATDNPTQNCVPKGMPTIMEAPYPIE
                     LVREANGDIRLKIEEYDLERHIVMNPSVTRELTSPTPLGDSVGRWDGATLVVTTTRIS
                     WPFFSQLGIRQSPAVRIVERFTPWDDGSRLDYEMTVTDPATFTRPVVLTQHWLWLPSV
                     TLLPYECTVR"
     gene            complement(1113..1886)
                     /locus_tag="VFL84_13575"
     CDS             complement(1113..1886)
                     /locus_tag="VFL84_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7609704.1"
                     /translation="MRALLLVASALLLAGAGQSCRAQDAPAATPAYAIPAGTPAHIRR
                     AVESPERNAEARARDYHRKPAEILTMSGIEEGDKIVEIASFGQYFTTMLSAAVGPTGR
                     VDMYDLPYTERFAGAASREFDEKHSNAVYHQESYNDVALPQNVDSVWMVLYYHDLKPA
                     GVDTAAFNRKIFNALKPGGIYLVIDHKAEDGSGWRDAGTIHRMGVDTIKQEVLAAGFE
                     LKQESDLLANPADDRTKMVFEPGTRGTTDQAFFIFRKPN"
     gene            2103..>2792
                     /locus_tag="VFL84_13580"
     CDS             2103..>2792
                     /locus_tag="VFL84_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609705.1"
                     /translation="MQTSRSAPAGISDPGSIDAKTGLEIERLRFAPEAEAEFTTYFIQ
                     RSLARARWATAIYLALVAVVTAINMRGEMAPLGDTLQQPIYLLRLGVACPALVLILAA
                     TVIPELQRHYQWITATAVIVTGMSVMAISGIAAANSLPQFQMGDVLVVVYATLFLGLL
                     ARPVVVVASALMISFTGIGWFLGVSTEHLIFASSVVFATTLMSVLSALRMERLLRANF
                     IENRLLNDIAER"
BASE COUNT          404 a          949 c          921 g          518 t
ORIGIN      
        1 gccggcgcca tgcgccagca cgagcgcggc cgcggcgtcg ttcggacatt gccacagcat
       61 gatgcgagcg ccgctagcgg accgtgcact cgtacggcaa cagcgtcacg ctcggcaacc
      121 acagccagtg ctgcgtgagc acgacaggcc gcgtgaacgt cgcggggtcc gtgacggtca
      181 tttcgtaatc gagacgcgag ccgtcgtccc acggcgtgaa gcgctcgacg atccgcaccg
      241 ccgggctttg cctgatgccg agctggctga agaacggcca gctgatgcgc gtcgtcgtca
      301 cgacgagcgt cgctccgtcc cagcggccca ccgagtcgcc gagcggcgtc ggtgaggtga
      361 gctcgcgcgt cacgctcggg ttcatgacta tgtgccgctc cagatcgtat tcctcgatct
      421 tcaggcggat gtcgccgttc gcttcgcgca cgagctcgat cgggtacggc gcctccatga
      481 tcgtcggcat gcctttcggc acgcagttct gggtcgggtt gtcggttgcg cggtcgaact
      541 tctgaagctg cgcgcgcgcc gccttagtca gcggataggc attgatgtcg acggtcgggt
      601 ccgtgacctc cgggaacagc cgcgcgatgg cgcgcgtgtg gctccagacg cggaagatgc
      661 cgaggtcggg ccgcgagccg tcgccttcgc gcgccatcag caacgagtcg tcgccgaccg
      721 tcgcgccgcg cttcgtccag cgcggcgtga gcccgacgtt catgagcagc tcgcggccgt
      781 cggcgagcag cacgtgccgc gcgtacatct ctttcttttc gccgagcggc gggttcgccg
      841 cgaggcggat tcgatcgcca acttcgatcg tgccttcatc gatccccatc cgcttcagca
      901 ccgtcagcga gctcgtctcg acgtcccagt cgacgccgtc gacgcgcacg ccgaaatagg
      961 cgtgcggatt gcgccacagc acttccgtga cctcgccctc gacctcgacg agcttttgcg
     1021 tgtcgaagcg gccgaaaaag ctgtggtgcg ccgcggcgac gctcggcagc gccgcgagag
     1081 cgatcaagcc gcgcgcgagc gcgtgcatcg gctcagttcg gcttgcggaa gatgaagaac
     1141 gcctggtccg tcgtgccgcg agtgccgggc tcgaacacca tcttcgtgcg gtcgtcggcc
     1201 gggttcgcga gcaagtcgct ctcctgcttc agctcgaagc cggcggcgag aacctcttgc
     1261 ttgatcgtgt cgacgcccat gcgatggatc gtgccggcgt cgcgccagcc cgagccgtcc
     1321 tcggccttgt gatcgatgac gagatagatg ccgccgggct tcaacgcgtt gaagatcttg
     1381 cggttgaacg ccgcggtgtc gacgcccgcc ggcttcaagt cgtggtagta gagcaccatc
     1441 cagacggagt cgacgttctg cggcagcgct acgtcgttgt agctctcttg gtggtagacg
     1501 gcgttcgagt gcttctcgtc gaactcgcgc gacgcagcgc ccgcgaatcg ctccgtatac
     1561 ggcaggtcgt acatgtcgac gcggccggtc ggcccgacgg ccgccgacaa catcgtcgtg
     1621 aagtactgcc cgaacgaggc gatctcgacg atcttgtcgc cttcctcgat gcccgacatc
     1681 gtcaggatct cggccggctt gcggtggtag tcgcgcgcgc gtgcctcggc gtttcgctcc
     1741 ggcgactcga cggcgcgcct tatgtgggcc ggcgtgcccg ccgggatcgc gtaggccggt
     1801 gtcgcggccg gcgcatcctg ggcgcggcag ctttgccctg ccccggctag gagcaaggcc
     1861 gatgccacga gcagcaatgc acgcatgagg accccctatt ggtctcgttg tcgggcctca
     1921 ttctgcgtgc tttcggcgcc cgagtgaact gccgcttccg gccttgcgcc gcgcgtaaaa
     1981 cggtgcgaaa gccgcgccgc cggccgctcc atcggctcga gttgagcacc gctgcacaat
     2041 tcgggggtgc cccttgtgct accgtgcgca cgtcacgcat ctgcccccgg agggccgctt
     2101 tcgtgcagac ctcgcgatcg gcgccggctg gcatttccga ccccggctcc atcgacgcaa
     2161 agaccgggct cgagatcgag cggctgcgct tcgctcccga ggcggaggcc gagttcacga
     2221 cctacttcat ccagcgttct ctggcccgcg cgcgctgggc gaccgcgatt tacctcgcgc
     2281 tcgtcgccgt cgtgacggcc atcaacatgc gcggcgaaat ggcaccgctc ggcgatacgt
     2341 tgcagcagcc gatttacctg ctgcgcctcg gcgtcgcgtg tccggcgctc gtgctgattc
     2401 tcgcggcgac cgtgatcccc gagctgcaac gccactacca atggatcacg gcgacggccg
     2461 tcatcgtgac cggcatgagc gtcatggcca tcagcggcat cgcggccgcg aacagcctgc
     2521 cgcaattcca gatgggcgac gtgctcgtcg tcgtctacgc gacgctgttc ctagggctct
     2581 tggcgcgccc cgtggtcgtg gtcgcgtcgg cgctgatgat ttcgttcacc ggcatcggtt
     2641 ggttcctagg cgtctcgacc gagcatctga tcttcgcgtc gagcgtcgtg ttcgcgacga
     2701 cgctgatgtc cgtgctgtcg gcattgcgca tggagaggct gctgcgcgcg aacttcatcg
     2761 agaaccggct gctcaacgac atcgccgagc gc
//
LOCUS       DASSNN010000723         3290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_862597, whole genome shotgun sequence.
ACCESSION   DASSNN010000723 DASSNN010000000
VERSION     DASSNN010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862597"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFL84_13585"
     CDS             <1..331
                     /locus_tag="VFL84_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7609706.1"
                     /translation="CWSALFFFASAAASSAYLTVSEIFPLEMRAVAISLFYAIGTGIG
                     GFIGPALYGTLIGTGSRDAVFFGYAVAGALMAGAAITAAWLGIDAERKPLEAVARPLS
                     AALEETQ"
     gene            424..1377
                     /locus_tag="VFL84_13590"
     CDS             424..1377
                     /locus_tag="VFL84_13590"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET7609707.1"
                     /translation="MPSRLSKLSIDEIRERYARSDEPVSAQTLSKLKRDPRHGVRVIY
                     EALKKRYERDSAERNRLDAMLNFERVLWRAGVQHIAGVDEVGMGPLAGPVIAAAVVFP
                     PHTELNGIDDSKKLDLEQRLDAESRIREAASAIAIGRAEVAEIDTVNIYHAGLLAMRR
                     AVEALPVRPQHVLVDARSIPGVDVPQNCFDKGDGIDFSIAAASIVAKVHRDRLMDELA
                     REYPEYGFERHKGYCTPEHQDAIRRLGPCAIHRKSFTFIRELRGEYSALFYDLKQRLY
                     AAAAAAELEAIEQEVDASRGALSESEQRKLRLTLSRRWRAL"
     gene            1362..2621
                     /locus_tag="VFL84_13595"
     CDS             1362..2621
                     /locus_tag="VFL84_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HET7609708.1"
                     /translation="MAGTLKKRELRAAARSADLRYVSDADRGLARRRRGKSFVYLRPG
                     GQVLRDKAALTRIRALAIPPAWDEVWICPWANGHLQATGRDARGRKQYRYHARWREAQ
                     DQNKYERIVAFAKALPAMRRAVARDLRKRGLPREKVLATTVKLLETTLIRVGNDEYAR
                     DNRSFGLTTMHDKHVKVRGAAIRFDFRGKHGIEHEIDLEDRRLARIVHACRDLPGQEL
                     FQYLDEAGDVCDVGSGDVNDYLRAISGQDFTAKDFRTWAGTALAAQALQEIQDFDTAA
                     AAKRNVTKAIERVAERLGNTQAICRKSYVHPAIIEAYMDRSLVKTSKRRAAKALRGSL
                     HRLSSEEAAVLALLQRRMEHELTGSPKPRTRATSKRAGRGTKRSGGPRRRSTTERRTA
                     GSCRPRSGCSPRRRTRASRAAPSRGAA"
     gene            complement(2518..>3290)
                     /locus_tag="VFL84_13600"
     CDS             complement(2518..>3290)
                     /locus_tag="VFL84_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609709.1"
                     /translation="GARAMKASFVSSAFAALAMAAAAASAQDVPRMADGKPDFSGIYT
                     PPSTVNATGPRGNLIFNADKMAPVKPGAESLLYEARNGDPRHDEPRAMCLPAGFPSGM
                     LYILPIQIVQNPNYIVIIPELQRAARIIPTDGRAHRTGIEPTYYGDSVGKWEGDTLVI
                     DSVNFKRWILDDYHYTDPTKTRWHSDELHTIERLTRDGNKLRYQITIDDPKIFTRPFS
                     QDFELTLRPDWEPLGLLEYVCEENNRCAGGNCRPSDVQ"
BASE COUNT          558 a         1089 c         1119 g          524 t
ORIGIN      
        1 gtgctggtcg gcattgttct tcttcgcatc ggcggccgcg agctccgcgt acctcacggt
       61 cagcgaaatc tttccgctcg agatgcgcgc ggttgccatc tctctcttct atgccattgg
      121 aacgggaatc gggggattca tcgggcccgc gctgtacggc acgttgatcg gtaccggcag
      181 ccgcgatgcg gtgttcttcg gctacgccgt cgccggggcg ctgatggcgg gcgccgcgat
      241 taccgccgcg tggctcggca tcgatgccga gcgcaagccg ctcgaagcgg ttgcgcgccc
      301 gctatccgca gccctcgaag aaacgcagta gcaccggcga cgtcgcaagc ccctcgatgg
      361 caagtcttgt ggctcgaggc cgcgttggct aagttgcggg cttcggagac ttgggaaccc
      421 agcgtgccgt ccaggctgtc gaaactatcc atcgatgaga ttcgtgagcg ctacgcgcgc
      481 tccgacgagc ccgtcagtgc gcagacgctg agcaagctca agcgcgaccc tcggcacggc
      541 gtgcgcgtga tctacgaggc gctcaagaag cgctacgagc gcgacagcgc cgagcgcaac
      601 cggctcgacg cgatgctgaa tttcgagcgt gtgctgtggc gcgccggcgt ccagcacatc
      661 gccggcgtcg acgaggtcgg catgggaccg ctggccggcc ccgtgatcgc cgcggccgtg
      721 gtattcccgc cgcacaccga gctcaacggc atcgacgatt cgaagaagct cgacctcgag
      781 cagcgcctcg acgccgagag ccgcatccgc gaggccgcga gcgccatcgc catcggccgc
      841 gccgaggtcg ccgagatcga caccgtcaac atctatcacg ccggtctact cgcgatgcgg
      901 cgagccgtcg aagcgctgcc ggtgcggccg cagcacgtgc tcgtcgacgc gcgcagcatc
      961 ccgggtgtcg acgtgccgca gaactgcttc gacaagggcg acggcatcga cttctcgatc
     1021 gccgccgcct cgatcgtcgc gaaggtccac cgcgaccgcc tcatggacga gctcgcgcgc
     1081 gagtatccgg aatacggctt cgagcggcac aaggggtact gcacgcccga gcaccaagac
     1141 gcgatccgcc ggctcggacc ctgcgcgatt catcgcaagt ctttcacctt cattcgcgag
     1201 ctgcgcggcg agtactcggc tttgttctac gatttgaagc agcgcctcta tgcggccgcg
     1261 gcggcggccg agctcgaagc gatcgaacag gaagtcgacg cgagccgcgg cgcgctctcg
     1321 gaaagcgagc agcgcaagct acggttgacg ttgtcacggc gttggcgggc actctaaaga
     1381 agcgcgagct ccgggcggct gcgcgttcgg ccgaccttcg ttacgtcagc gacgcggacc
     1441 gcgggttggc tcggcgccgc cgcggcaagt cgttcgtcta tcttcggccg ggcggccaag
     1501 tgctgcgcga caaggcggca ctcacgcgga ttcgcgcgct ggccattccg ccggcctggg
     1561 acgaggtctg gatttgcccg tgggcaaacg gacacctgca ggcgacgggc cgcgacgcgc
     1621 gcggccgcaa gcaataccgc tatcacgcgc gctggcgcga ggcccaggac cagaacaagt
     1681 acgaacgaat cgtcgcgttc gcgaaagcgc tgcccgctat gcgccgcgcc gtcgcgcgcg
     1741 acttgcgcaa gcgcgggctg ccgcgcgaga aagtgctcgc gacgacggtc aagctgctcg
     1801 agacgacgct gattcgcgtc ggcaacgacg aatacgcccg cgacaatcgc tcgttcgggc
     1861 tgacgacgat gcacgacaag cacgtcaagg tgcgcggcgc ggcgatacgg ttcgactttc
     1921 gcggcaagca cggcatcgag cacgaaatcg acctcgagga ccggcggctg gcccggatcg
     1981 tccatgcgtg ccgcgatctg cccgggcaag agctcttcca gtacctcgac gaggcgggcg
     2041 acgtctgcga cgtcggctcc ggcgacgtca atgactatct gcgcgccatc agcgggcagg
     2101 atttcacggc caaggacttc cgcacttggg ccggcacggc gctcgccgca caagcgctac
     2161 aggagatcca ggacttcgat acggcggccg ccgcgaagcg caacgtcacg aaggcgatcg
     2221 agcgcgtcgc cgagcggctc ggcaacacgc aagcgatctg ccgcaagagc tatgtgcatc
     2281 cggcgatcat cgaagcctac atggatcgct cgctcgtcaa gacgtcgaag cggcgagccg
     2341 cgaaggcgtt gcgcggctcg ctgcaccggc tgtcgtccga ggaagcggcc gtgctcgcac
     2401 tgctgcagcg gcgcatggag cacgagctga ccggcagccc aaaaccccga acgcgagcga
     2461 cgtccaaacg cgcgggccgg ggcaccaagc gcagtggcgg gccgcgccga aggtcgacta
     2521 ctgaacgtcg gacggccggc agttgccgcc cgcgcagcgg ttgttctcct cgcagacgta
     2581 ctcgagcaag ccgagcggct cccagtcggg gcgcagcgtg agctcgaagt cttgcgagaa
     2641 cgggcgcgtg aagatcttcg ggtcgtcgat cgtgatctgg taacgcagct tgttaccgtc
     2701 gcgagtcagc cgttcgatcg tgtgcagctc gtcgctgtgc cagcgcgtct tcgtcgggtc
     2761 cgtgtagtga tagtcgtcca agatccagcg cttgaagttc acgctgtcga tgacgagcgt
     2821 atcgccttcc cacttgccga ccgaatcgcc gtagtacgtc ggctcgatgc cggtgcgatg
     2881 cgcgcggccg tccgtcggaa tgatgcgagc cgcgcgctgc agctcgggaa tgatcacgat
     2941 gtaattcggg ttctgcacga tctgaatcgg caggatgtag agcatcccgg agggaaaccc
     3001 ggccggcagg cacatggcgc gcggctcgtc gtggcgcggg tcgccgttgc gcgcctcgta
     3061 gagcaagctc tcggcgcccg gcttcacggg ggccatcttg tccgcgttga agatcaggtt
     3121 gccgcgcggg cccgtcgcgt tcacggtcga aggcggcgtg tagatgccgg aaaagtccgg
     3181 cttgccgtcc gccatgcgtg gaacgtcctg cgcgcttgcg gccgccgccg ccatggcgag
     3241 cgctgcgaat gcgctggaga cgaatgaagc tttcatcgcg cgggcgcctc
//
LOCUS       DASSNN010000724         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1634602, whole genome shotgun sequence.
ACCESSION   DASSNN010000724 DASSNN010000000
VERSION     DASSNN010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634602"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VFL84_13605"
     CDS             complement(<1..1100)
                     /locus_tag="VFL84_13605"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET7609710.1"
                     /translation="MASALAIVAVEPVAAQEQAQVAQPLEEVVVTGSRITRQDFSANS
                     PITTIDRTTFENTSTIGVETVLNQLPQFIPAVTQFTTGDVQQTGDRTIGASTVSLRGL
                     GANRNLVLIDGKRGQPVDPTMVVDTNMIPAAAIQRVEIISGGASAVYGADAVGGVVNF
                     ILKDNFEGASVDVRVGDTEHGGNQEITISGLIGVNAGDRGNVMVGVERSSRSKQLTWE
                     RDWRIEDMANPSTAGTAFGWGSDTWISNIQGTFGNNPSQAFVNNMFSQAQPCAFGDPP
                     GPAGPNEWATGVCPTVPAGQPLEGAVLGVSNSNATRFLLNRSPDPAQRTVYTGLMQSS
                     GAAGSYRYNGPFNVDNLGNFEGLPYRAYQPDGT"
     gene            complement(1488..>2066)
                     /locus_tag="VFL84_13610"
     CDS             complement(1488..>2066)
                     /locus_tag="VFL84_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7609711.1"
                     /translation="VVSVWSFRRNLNWYVLGWTLPGATLGILIGYLVAASVSSAAVMA
                     LVGGISIAFGGYRLWTERHGPPTAGASSPGWLGSLAGVASGFTSQIAHAGAPPFQLWV
                     LPRRLPRDVLIGTWAVYFAALNWIKVPAFAALGQFTATNMLTAAALLPVAIGSTFAGV
                     WLVRRIDGERFYKIIYRLMILVGIILIGEAVL"
BASE COUNT          359 a          679 c          687 g          341 t
ORIGIN      
        1 gtgccgtcgg gctgataggc gcggtacggc aggccttcga agttgccgag gttgtcgacg
       61 ttgaaggggc cgttataccg atacgagccg gcggcgcctg agctctgcat gaggcccgta
      121 tagacggtgc gctgcgccgg atccgggctt ctgttcagca ggaatcgcgt tgcgttggag
      181 ttcgaaacgc cgagcacggc gccctccaac ggctgaccgg cggggaccgt cgggcacacg
      241 ccggtcgccc attcgtttgg gcccgcggga ccgggcggat cgccgaacgc gcacggctgc
      301 gcttgcgaga acatgttgtt gacgaatgcc tggctcggat tgttgccgaa cgtgccctgg
      361 atgttgctga tccaggtatc gctgccccag ccgaacgccg tgccggccgt gctggggttc
      421 gccatgtcct cgatgcgcca gtcccgttcc caggtgagct gcttcgaccg cgacgagcgc
      481 tcgacgccga ccatcacgtt gccgcggtcg cccgcgttca cgccgatgag gcccgagatc
      541 gtgatctctt ggttgccgcc gtgctccgtg tcgcctacgc gcacgtcgac ggaggcgccc
      601 tcgaagttgt ccttcaagat gaagttgacg acgccgccga cggcgtccgc gccgtacacg
      661 gccgaagcgc cgcccgagat gatctccacg cgctgaatgg cggcggcggg aatcatgttc
      721 gtgtcgacga ccatcgtcgg atcgacgggc tgcccgcgct taccgtcgat cagcacgagg
      781 ttgcggttcg cgccgagccc gcggaggctg acggtcgacg caccgatcgt tcgatcgccc
      841 gtctgctgca cgtcgccggt cgtgaactga gtgacggccg gaatgaactg cggcagctgg
      901 ttcagcaccg tctcgacgcc gatcgtgctc gtgttctcga acgtcgttcg atcgatcgtc
      961 gtgattgggc tgttggcaga gaagtcttgg cgcgtgatgc gcgagccggt gacgacgacc
     1021 tcctcgagcg gttgagctac ttgcgcttgc tcctgtgcgg cgaccggctc gacggctacg
     1081 atcgccaatg ccgaggccaa cagcgcggtc ccggatgccg gggccgtgat tcgtctcgtt
     1141 cttcgccctg ccggcattct ctgcagcgct tcggcaacgg caacgggcaa gactgcggcg
     1201 cgcaggcgcg cgcgcctatg gatacatgcg ttcatgaagc cccccaatca tgcgtgtttt
     1261 gtttcagcga ctccggctaa ggacacgcgc tcgacgcttg cgaccgcgac accccgccgg
     1321 aacgagccga caccccccga aggcgtcgtg ctagggttcg agcttgccgc agaacgccga
     1381 gggttgcgcg ccgttgccgg aaacgaatag cgacgtcacc ggaagcgagc gccgcggagt
     1441 gctgatgttg ctcgacgtcg ccggcgacta actgccggcg ccgtcggtca gagcacggct
     1501 tcgccgatca agatgatgcc caccaagatc attaaccgat aaatgatctt gtagaaccgt
     1561 tcgccgtcga tgcggcgcac gagccaaacg cccgcgaaag tcgagccgat cgcaacgggg
     1621 agaagcgccg cggccgtcag catgttcgtt gcagtgaact gaccgagcgc cgcgaacgcg
     1681 ggcactttga tccagttcag cgccgcgaag tacacggccc acgttccgat caagacgtcg
     1741 cgcggaaggc gccgcggcaa gacccaaagc tggaacggcg gcgcgcctgc atgcgcgatc
     1801 tggctcgtga atcccgaagc cacgccggcc agcgaaccga gccagcccgg cgacgaagcg
     1861 ccggcagtgg gcggcccgtg ccgttcggtc caaagacgat agccgccgaa ggcgatcgag
     1921 atgccgccca cgagcgccat gacggcggcc gacgagacgc tcgcggcgac caagtagccg
     1981 atcaagatcc caagcgtcgc gccgggcaac gtccagccga gcacgtacca attcaggtta
     2041 cggcgaaacg accacacgct gacgac
//
LOCUS       DASSNN010000725         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1329673, whole genome shotgun sequence.
ACCESSION   DASSNN010000725 DASSNN010000000
VERSION     DASSNN010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329673"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1757
                     /locus_tag="VFL84_13615"
     CDS             <1..1757
                     /locus_tag="VFL84_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET7609712.1"
                     /translation="AVMDRIGHSVYGAVTALQARLGDLQIWWEGRRAKQQRDEVVTKV
                     RAKSKTTAPRIEPVIEKIEVSARATERLERERQVPMFEPAASNELPALSLLDNPPPKE
                     GGYSAEALEAMSRLVELKLNDFGVEAEVVAVHPGPVVTRFEIKPAAGVKSSQISGLAK
                     DLARSLATVSVRVVEIIPGKPYVGLEIPNEVREIVALGEIVKSQAYDQLKSPLTLALG
                     KDISGQPMVADLARMPHLLIAGTTGSGKSVALNAMVLSLLYKSTPEHVRLIMIDPKML
                     ELSVYEGIPHLLAPVVTDMKQAANALRWCVAEMERRYALMAGLGVRHLSSYNKKVKEA
                     LENGTPLTDPTFIPETEDDKAPELHPMPYVVVIIDELADMMMMVGKKVEELIARLAQK
                     ARASGIHMIVATQRPSVDVITGLIKANIPCRIAFQVSAKVDSRTILDQIGAEHLLGHG
                     DMLYLPSGTSMPIRVHGAFVSDNEVHAVVKALRASGEPNYIDNVLDGPAGELAGVEAP
                     GPTGELDGEQDPLYDQAVRIVTETRKASISGIQRRLKIGYNRAARMVETMEEAGIVGN
                     LQTNGFREVLAPPPPVAD"
     gene            1711..2376
                     /gene="lolA"
                     /locus_tag="VFL84_13620"
     CDS             1711..2376
                     /gene="lolA"
                     /locus_tag="VFL84_13620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HET7609713.1"
                     /translation="MVSGKYSRRRLRSLTSLVFLFSVAAIHGASAQSAGDGIARLEAF
                     LDGVHSLTARFKQEIWSEDQTRLEQTDTGSLWLQRPNRFRWTYEGPAELVVVADGTKL
                     WIYDKELEEVTVTPFDDTAGASPAVLLSGDRNVREDFEVVESYTRDGLDWVKLAPKKA
                     SDFSAISIGFNGTEPRRLELVDGLGQTTRVELDDLVLNPEIPDDVFHLDVPPGVDVSG
                     NDG"
BASE COUNT          453 a          814 c          825 g          409 t
ORIGIN      
        1 tcgccgtcat ggatcggatc ggccacagcg tgtatggcgc ggtgacggcg ctgcaggcgc
       61 ggctaggcga tctgcagatc tggtgggaag gccggcgagc caagcagcag cgcgacgaag
      121 tcgtgacgaa agtgcgggca aagtcgaaga cgacggcgcc gcgcatcgag cctgtcatcg
      181 agaagatcga agtcagcgcg cgggcaacgg aacggctcga gcgcgagcgg caagtgccga
      241 tgttcgagcc ggcggcgtcg aacgagctgc cggcgctgtc gttgctcgac aacccgccgc
      301 cgaaggaagg cggctattcc gctgaggctc tggaggcgat gtcacggctc gtcgagctca
      361 agctcaacga tttcggcgtc gaagccgagg tcgtcgccgt acatcccggc cccgtcgtca
      421 cacggttcga gatcaagccc gcggccggcg tgaagagcag ccagatcagc ggtctcgcca
      481 aggacctcgc gcgctcgctt gcgacggtca gcgtccgcgt cgtcgagatc attcccggca
      541 agccgtacgt cggcctcgag attccgaacg aagtgcgcga gatcgtcgcg ctcggcgaga
      601 tcgtgaagtc gcaagcgtac gaccagctga aatcgccgtt gacgctcgcg ctcggcaagg
      661 acatcagcgg ccagccgatg gtcgccgatc tcgcgcgcat gccgcacctc ttgatcgccg
      721 gcacgaccgg gtcgggcaag tccgtcgcgc tgaacgcgat ggtgttgagt ctcctgtaca
      781 agtccacgcc cgagcacgtg cggctcatca tgatcgatcc gaagatgctc gagctctcgg
      841 tgtacgaggg cattccgcat ctcctggcgc ccgtcgtcac ggacatgaag caggccgcga
      901 acgcgctgcg ctggtgcgtc gcggagatgg agcggcgcta cgcgctgatg gcgggcctcg
      961 gcgtgcgtca tctgtcgagc tacaacaaga aggtcaagga agcgctcgag aacggcacgc
     1021 cgctcaccga tccgaccttc atccccgaga ccgaggacga caaggcaccc gagctgcacc
     1081 cgatgccgta cgtcgtggtc atcatcgacg agctcgccga catgatgatg atggtcggca
     1141 agaaggtcga ggagctcatc gcgcggcttg cgcagaaggc gcgcgcttcc ggcatccaca
     1201 tgatcgtcgc gacgcagcgg ccgtcggtcg acgtgatcac gggcctgatc aaggccaaca
     1261 ttccgtgccg catcgcgttc caggtgtcgg ccaaggtcga ctcgcggacg atcctcgatc
     1321 agatcggcgc cgagcatctg ctcggccacg gcgacatgct gtatctgccg tcgggcacct
     1381 cgatgccgat ccgcgtgcac ggcgcattcg tctccgacaa cgaggtccac gccgtcgtca
     1441 aagcgctgcg cgcgagcggc gagccgaact acatcgacaa cgtcctcgac ggtccggccg
     1501 gcgagctcgc cggagtcgaa gcgccggggc cgaccgggga gttggacggc gagcaagatc
     1561 ccctctacga tcaagccgtc cggatcgtca cggagacgcg caaggcgtcg atctccggca
     1621 tccagcggcg gttgaagatc ggctacaacc gcgctgcgcg gatggtcgag accatggaag
     1681 aggctggcat cgttggcaat ctgcagacga atggtttcag ggaagtactc gcgccgccgc
     1741 ctccggtcgc tgactagcct cgtctttctt ttctccgtcg cagccattca cggcgcgtcg
     1801 gcgcagtccg ctggcgacgg cattgcgcgg ctcgaagcat ttctcgacgg cgtccactcg
     1861 ttgacggcgc gcttcaagca ggagatctgg agcgaggatc aaacgcggct cgagcagacc
     1921 gacacaggat cgctgtggct gcaacgccct aatcggttcc gctggaccta tgaaggtccc
     1981 gcggagctcg tcgtcgtcgc cgacggtacg aagctctgga tctacgacaa agagctcgag
     2041 gaagtcaccg tcacgccgtt cgacgacacc gccggcgcga gcccggcggt gctgctcagc
     2101 ggcgatcgca acgtccgcga agacttcgag gtcgtggagt cgtacacgcg cgacgggctc
     2161 gattgggtca agctcgcgcc gaagaaagct tccgactttt cggcgatctc gatcggcttc
     2221 aacggcaccg agccgcgccg gctcgagctc gtcgacggat tgggccagac cacgcgcgtc
     2281 gagctcgacg atctcgtgct caatcccgag attcccgacg acgtgttcca tctcgacgtg
     2341 ccgcccggcg tcgacgtaag cggtaacgac ggctgagacg acgtgctgcc gctgcgtttt
     2401 ccgaggctct ggacggcgct cgcctggtcg ctgaccgcgg ccgtcatcat cggcagcctc
     2461 cttccgggtc ccgtcgtcgc gtccattccg attcgcgaca a
//
LOCUS       DASSNN010000726         4620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_862904, whole genome shotgun sequence.
ACCESSION   DASSNN010000726 DASSNN010000000
VERSION     DASSNN010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862904"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VFL84_13625"
     CDS             <1..1248
                     /locus_tag="VFL84_13625"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HET7609714.1"
                     /translation="PFHDLLAPSIHYAREGFPVTEVIGRAWAAGARVLHAYPGFAEVF
                     MPNGGAPEVGEPFANPGLARAYELIASEGREPFYDGAIAAAIEDCVRENGGYLGREDL
                     RAHRCEWVEPVSTTYRGWRVFELPPNGQGITVLQMLNILEGFELGSMKWGSAEHLHLL
                     LEAKKLAFEDRAKFYADPAFAALPVERLVSKEYAAARRALIDVNRAAESPTHGDLRPG
                     AGDTVYLAAADADGNMVSLIQSNYMGFGSGVTVAPFGFGLQNRGSQFALDPKHANAYA
                     PGKRPFHTIIPAFAMRSGEALLAFGVMGAAMQPQGHVQVLTNMLDFGLGLQGAGDAPR
                     VRHEGSTEPTGAPRQMHGGTVYLEPGFAAETIDGLRARGHRVEPIEDRAAFGGYQAIK
                     RDLGRGVYLGASESRKDGHAAGY"
     gene            complement(1462..4602)
                     /gene="putA"
                     /locus_tag="VFL84_13630"
     CDS             complement(1462..4602)
                     /gene="putA"
                     /locus_tag="VFL84_13630"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HET7609715.1"
                     /translation="MFDAAHPAPPVVSAAITDRYLADEGTVVAELVERAALAPAQSAR
                     VRREAAALVEAIRRRQQDAGGVDALLRAYDLSSGEGIVLMCLAEALLRIPDADTADRL
                     IADKLGSADWQAHLGKSESLFVNASTWALLLTGRIVRPAELDEAPASFFSRLVERVGE
                     PVVRAALRQAMRILGSQFVMGETIDGALRRAAEHPELNYSFDMLGEAALTAADAQRYF
                     EAYGAAIEAVGHGGASAGSLLDAPSVSVKLSALCPRFEVPQSARAVRELVERLTELAL
                     AASAARIGLTVDAEEADRLELSLAIFAAVFGDERLDDYFGLGLAVQAYQKRALPVLEW
                     LAALARGAGRTIPVRLVKGAYWDSEIKRAQEQGLDDYPVFTRKCNTDVSYLACAKALL
                     TEHDSLYPQFATHNAHTVAWLLEVGGGRPFELQRLHGMGEELYGELNARPSFTHRARV
                     YAPVGSHEHLLPYLVRRLLENGANTSFVNRLVQAEADVDEIVADPVEAVRALGGSVRH
                     PRIPLPRALFAPARENSRGTKLASSRDLEGLAAALAATGAGAWRARPRIDGEDVDGAP
                     QECRDPADRRRLVGIAELADGKHARRALDAASAYAPRFSETPAAERAALLKRAADLFE
                     ADRAELAARCVAETGKTVPDSLNEVREAVDLLRYYAAECERVLARPMRLPGPTGERNE
                     LELVGRGVFFCVSPWNFPLAIFTGQVAAALAAGNAVIAKPAEQGTLVAARAVALLERA
                     GIPAGALQFLPGDGRALGEVALADPRVAGVAFTGSVETARAINAALAQREGALATLIA
                     ETGGVNAMIVDSSALPEQVVRDALASAFNSAGQRCSALRLLCLPEHTADEILRMLCGA
                     MDELVVGDPQLLATDVGPVIDADAHRALTDYVGERERSVVHRVRLGAAHEHGLFVPPT
                     VVALERASQLTREVFGPVLHVVRYDPRRLDALVDEINGLGYGLTLGVHTRIDSTADRV
                     ARRARVGNVYVNRNMIGAVVGVQPFGGMGLSGTGPKAGGPHYLPRFATERTVTTNTAA
                     VGGNATLLSLDATPGT"
BASE COUNT          677 a         1641 c         1662 g          640 t
ORIGIN      
        1 ccgttccacg acctgctcgc gccgtcgatt cattacgccc gcgagggttt tcccgtgacc
       61 gaagtcatcg gccgcgcatg ggcggccggc gcgcgcgtgc tgcatgcgta tcccggcttc
      121 gccgaggtgt tcatgccgaa cggcggcgcg ccggaggtcg gcgagccatt cgcgaacccg
      181 gggctggcgc gcgcctacga gctcatcgcg agcgaaggcc gcgagccgtt ctacgacggc
      241 gcgatcgctg ccgccatcga agactgcgtg cgcgagaacg gcggatatct cgggcgcgag
      301 gatcttcgcg cgcaccgctg cgagtgggtc gagcccgtct cgacgactta tcgcggctgg
      361 cgtgtcttcg agctgccgcc gaacggccag ggcatcacgg tccttcagat gttgaacatc
      421 ctcgaagggt tcgagctcgg ctccatgaag tggggcagcg cggagcacct acacttgctt
      481 ctcgaggcga agaagctcgc attcgaggac cgcgcgaagt tctacgcgga ccccgcgttc
      541 gcggcgctac ccgtcgagcg gctcgtctcg aaggagtacg cggcggcgcg ccgagcgctg
      601 atcgacgtga accgcgcggc cgagtcgccg acgcatggcg acttgcggcc gggcgccggc
      661 gacaccgtct acctcgcagc ggccgatgcc gacggcaaca tggtgtcgtt gatccagagc
      721 aactacatgg gcttcggctc cggcgtcacc gtcgcgccgt tcggcttcgg gctccagaat
      781 cgcggctcgc agttcgcgct cgacccgaag catgcgaacg cgtatgcgcc cggcaagcgg
      841 ccgttccaca cgatcattcc cgcgttcgcg atgcgctccg gtgaagcttt gctcgcgttc
      901 ggcgtcatgg gcgcggccat gcagccgcag ggccacgtgc aagtgctgac gaacatgctc
      961 gactttggac tgggcctgca aggcgccggc gacgcgccgc gcgtgcgcca cgagggctcg
     1021 accgagccca cgggcgcgcc gcggcaaatg cacggcggca ccgtgtatct cgagcccggc
     1081 ttcgcggcgg aaacaatcga cggcttgcgc gcgcgcggcc accgggtcga gcccatcgag
     1141 gaccgggcgg cgttcggcgg ctatcaagcc atcaagcggg acttgggccg cggcgtctat
     1201 ctcggcgcgt cggagtcgcg caaggacggg cacgcggcgg gttactgaag aacgtagtgg
     1261 gcagaaccgg gcgcggtgca gtgaaatcgt tggggggatg cgattcaggt gactaccgcg
     1321 accggccctg cccgcttcta cgtagcaaac ggcgtgccaa gtcgcaaagc caggcgattc
     1381 gggggtcgcg cgcaatgagc cgtgcgcgcg tgcacccgct tggagcaagc gcggcgcgcg
     1441 cttgcccgaa attggcgagg ctcaggtacc cggcgtcgcg tcgagcgaca acagcgtcgc
     1501 gttgccgccg acggcggccg tgttcgtcgt gaccgtgcgc tcggtcgcga agcgcggcag
     1561 gtagtgaggc ccgccggcct tcggccccgt gccggacagg cccatgccgc cgaacggctg
     1621 cacgccgacg acggcgccga tcatgttgcg gttgacatag acgttgccga cgcgcgcgcg
     1681 ccgcgcgacg cggtccgccg tcgaatcgat gcgcgtgtgg acgccgagcg tcagcccgta
     1741 cccgaggccg ttgatctcgt cgacgagcgc gtcgagccgc ctcgggtcgt aacgcacgac
     1801 gtgcagcacg gggccgaaca cttcgcgcgt cagctgcgaa gcgcgctcga gcgcgacgac
     1861 ggtcggcggc acgaacaagc cgtgctcgtg cgccgcgccg aggcggacgc gatgcacgac
     1921 gctgcgctcg cgctcgccga cgtagtcggt cagcgcgcga tgcgcgtccg cgtcgatgac
     1981 aggccccacg tccgtcgcga gcagctgcgg atcgccgacg acgagctcgt ccatcgcgcc
     2041 gcacagcatg cgcaggattt cgtccgccgt atgctcgggc aggcagagca agcgcagcgc
     2101 ggagcagcgc tgacccgcgc tgttgaacgc cgacgcgagc gcgtcgcgca cgacttgctc
     2161 gggcaacgcc gagctgtcga cgatcatcgc gttgacgccg ccggtttccg cgatcagcgt
     2221 cgcgagcgcg ccttcgcgct gggcgagcgc cgcgttgatc gcgcgcgcag tctcgacgga
     2281 gcccgtgaac gcgacgccgg cgacgcgcgg atcggcgagc gctacctcgc cgagagcgcg
     2341 gccgtcgccg ggaaggaact gcagagcgcc cgccgggatc ccggcgcgct cgagcagcgc
     2401 gaccgctcgc gccgcgacga gcgtgccctg ctcggccggc ttcgcgatca cggcgttgcc
     2461 ggccgcgagc gccgcggcga cctggcccgt gaagatcgcg agcggaaaat tccacgggct
     2521 cacgcagaag aatacgccgc gcccgacgag ctcgagctcg ttgcgttcgc ccgtcggccc
     2581 cggcaaccgc atcggccgcg cgagcacgcg ctcgcattcg gcggcgtagt agcgcagcag
     2641 atcgacggcc tcgcggacct cgttgaggct gtcgggcacc gtcttgcccg tctcggcgac
     2701 gcagcgcgcg gcgagctcgg cgcgatccgc ctcgaataag tcggccgcgc gtttcaacag
     2761 cgccgcgcgc tccgcggccg gcgtctcgct gaagcgcggc gcgtacgcgc tcgccgcgtc
     2821 gagcgcgcgc cgcgcgtgct tgccgtcggc gagctcggcg atgccgacga ggcgccgccg
     2881 atcggcgggg tcgcggcact cctgcggcgc gccgtcgacg tcctcgccgt cgatgcgcgg
     2941 ccgcgcgcgc cacgcgcccg cgccggtcgc cgcaagcgcc gcggcgaggc cctcgagatc
     3001 gcggctcgag gcgagtttcg tgccgcgcga gttctcgcgc gcgggcgcga acagcgcgcg
     3061 cggcagcgga atccgcgggt gccgtaccga gccgccgagc gcgcggacgg cttcgacggg
     3121 atcggcgacg atctcgtcga cgtcggcctc ggcctgcacg agccgattca cgaacgacgt
     3181 gttggcgccg ttctcgagca gccgccgcac gagatacggc aagagatgct cgtggctgcc
     3241 caccggcgcg tagacgcggg cccggtgcgt aaagctcggc cgcgcgttga gctcgccgta
     3301 gagctcctcg cccatgccgt gcagccgctg cagctcgaac ggcctgcccc cgccgacctc
     3361 gagcagccag gcgaccgtgt gcgcgttgtg cgtcgcgaat tgcggataca gcgaatcgtg
     3421 ctcggtcagc agcgctttcg cgcacgcgag gtacgagacg tcggtgttgc acttgcgcgt
     3481 gaacacgggg tagtcgtcga gcccttgctc ctgcgcgcgc ttgatctcgc tgtcccagta
     3541 agcgcctttg acgaggcgaa ccggaatcgt gcggcccgcg ccgcgcgcga gcgccgctag
     3601 ccactcgagc acgggcagcg cgcgcttctg ataggcctgc acggcgaggc cgagaccgaa
     3661 gtagtcgtcg aggcgctcgt cgccgaacac ggccgcgaag atcgcgagcg atagctcgag
     3721 gcggtcggcc tcctcggcat cgacggtgag accgatgcgc gcggcgctcg ccgcgagcgc
     3781 gagctcagtc agtctctcga cgagctcgcg cacggcgcgc gcgctctgcg gaacctcgaa
     3841 gcgcgggcag agcgccgata gcttcaccga aacgctcggc gcgtcgagca gcgagcctgc
     3901 gctcgcgccg ccgtgcccga cggcctcgat cgcggcgccg tatgcctcga agtagcgctg
     3961 cgcgtccgcc gccgtcagcg cggcctcacc gagcatgtcg aacgagtagt tgagctccgg
     4021 gtgctcggcc gcgcggcgca gcgcgccgtc gatcgtctcg cccatgacga actgcgagcc
     4081 caaaatccgc atcgcctggc gcagcgccgc gcggacgacg ggctcgccga cgcgctcgac
     4141 gagccgcgag aagaacgacg ccggcgcttc gtcgagctcg gccggacgca cgatgcggcc
     4201 cgtcaacagt agcgcccacg tggacgcgtt gacgaacagc gactcgctct tgcccaagtg
     4261 cgcctgccag tccgcggagc cgagcttgtc cgcgatgagc cggtcggccg tgtccgcgtc
     4321 ggggatgcgc agcagcgctt cggccaggca catcagcacg atgccttcgc ccgacgacaa
     4381 gtcgtaggcg cgcagcagcg cgtcgacgcc gcccgcgtct tgctgcctgc ggcgaatcgc
     4441 ttccacgagc gcggcggcct cgcggcgcac gcgcgcggac tgcgccggcg cgagcgcggc
     4501 gcgctcgacg agctcggcga ctacggtgcc ttcatcggcg agatagcgat cggtgatcgc
     4561 cgcgctgacg acgggcggcg cgggatgagc cgcgtcgaac agccaatggc gctccgatcc
//
LOCUS       DASSNN010000727         7511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_986017, whole genome shotgun sequence.
ACCESSION   DASSNN010000727 DASSNN010000000
VERSION     DASSNN010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986017"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            944..1429
                     /locus_tag="VFL84_13635"
     CDS             944..1429
                     /locus_tag="VFL84_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7609716.1"
                     /translation="MHLSANVFDVWVFRRRGAEAEFLLLYTSVEKATRHFNGGRFWQI
                     PSNVVNDGESIVEAIRRVLDRYGLRPKAIWAGEHAYLIYNRRFAEMQAIGVYAAEVED
                     AAVHLDPAEHSEFAWLSREDCLNRVHYRGLKDGLRSVHEYVTGVEAPAKELCLYIAPS
                     A"
     gene            1478..1954
                     /locus_tag="VFL84_13640"
     CDS             1478..1954
                     /locus_tag="VFL84_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7609717.1"
                     /translation="MTVKRMDNVGIVVENLDAAIEFFTELGLTLEGRMPIEGEWAGRV
                     TGVRGQRVEIAMLRTPDGHNRIELSRFDAPAIASDHRTAPVNSLGYLRAMFTVDDIDE
                     TLVRLEKLGAKVVDEVVDYEAIYRLCYIRGPEGILIGLAQQLGQQASRENPMERKR"
     gene            1977..2504
                     /locus_tag="VFL84_13645"
     CDS             1977..2504
                     /locus_tag="VFL84_13645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7609718.1"
                     /translation="MLSIRRATANDTGEIERMVADFVKDHPAANHPRDAAALRAAYFG
                     EHPVAQLLVAERNGEIVGMAQWRLIYDMFWGMFGAQAEWLYVKPQCRGSGIVAALVAR
                     VCAEASAAGAKFAQGGGGEGPSRLYERVAIGMSDRACHLSGKAFQVFAKLDGLPVREI
                     VRRLPAKELGLQPPD"
     gene            2945..3343
                     /locus_tag="VFL84_13650"
     CDS             2945..3343
                     /locus_tag="VFL84_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007128925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7609719.1"
                     /translation="MTTARPFLMFQGGVAQAALDLYFATFPDSRMVRVDRYAKGEPAP
                     EGTIKVAVFTICGREWMCSDSPIDHAFTFTPSSSTFVDFDSAAELDRVFGILVDGGKV
                     LMPLNDYGFSRRFGWLVDRFGVSWQLNLPA"
     gene            3340..3729
                     /locus_tag="VFL84_13655"
     CDS             3340..3729
                     /locus_tag="VFL84_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609720.1"
                     /translation="MTALDIRKLANAFIPAATPSDRLPSAALTAVTRFKRKHGGLWVG
                     GTVAATDRGVSFEPNGLNRAFHEDLETISVRAEDIRAVRHEFGWFTGVVVVTHAGGEF
                     RFRCYGAKKLAAALAAAYRERRSHAQT"
     gene            3716..4186
                     /locus_tag="VFL84_13660"
     CDS             3716..4186
                     /locus_tag="VFL84_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET7609721.1"
                     /translation="MPKHKARRVALDLRGLSGREELFRLSGAVKRDPAVERWLTSGAS
                     ELRSLAQRWFARMRECGADVRELMHDGCPTACVEGAAFAYVSTFKAHVNVGFFCGAQL
                     PDPAGLLEGSGKRMRHVKLRPGEEPNAAALDELIDAAYRAIKARLAAERAAADD"
     gene            4387..4620
                     /locus_tag="VFL84_13665"
     CDS             4387..4620
                     /locus_tag="VFL84_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609722.1"
                     /translation="MPQYRFNVFGRLVVIKGAPGNWSAFLLGADGKRRKADFLVPGSL
                     CEDELCGYLSDLFHESASSTQPAVTRLDCSSNT"
     gene            4678..5037
                     /locus_tag="VFL84_13670"
     CDS             4678..5037
                     /locus_tag="VFL84_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019666704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7609723.1"
                     /translation="MASTHHAIDYIEFTVRDLNAAKRFYAAAFGWQFNDYGPEYAGIK
                     GVDGEVGGLQQAAQPRTGGPLVVLYSKDLEKTLAAVRAAGGKIVREPFAFPGGRRFQF
                     TDPSGNELGVWSQAQDH"
     gene            5313..5966
                     /locus_tag="VFL84_13675"
     CDS             5313..5966
                     /locus_tag="VFL84_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609724.1"
                     /translation="MRLSALAISGAAVALAIALVAYFRPGGEPPLVSKSETPPVQEVP
                     VANEPAAAPPLEATVFVPPAAESRAGAGPVPSIEPAPLPGATATTPMADLMAGRGTDR
                     PMPALIERERAFAVEPVDRTWAPAAEARIYAKLAEIPGLKLIGLQVECRSTMCRIQTE
                     SSGGSSLPFKDLLGPAGLEPAWMATIKERNGPVRSAAYLWRDGFAPPTEPLQPRDTN"
     gene            6037..6465
                     /locus_tag="VFL84_13680"
     CDS             6037..6465
                     /locus_tag="VFL84_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7609725.1"
                     /translation="MAIDVRGVCTLVLVFDMPTSLKFYRDVLGFEVVATNDDSAGDNV
                     DWCYLRLGKAELMLNGMYEAHRRPAAPDKKRIATHSDVCLYFACPDVDGAYCYLRSKG
                     VDVKEPQVARYGMKQLYLLDPDGYNLCFQWEATPVEQPVP"
     gene            6462..6875
                     /locus_tag="VFL84_13685"
     CDS             6462..6875
                     /locus_tag="VFL84_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance family protein"
                     /protein_id="HET7609726.1"
                     /translation="MKVHRLTPILNVSDIQESFEWFEKLGWKKAWDWGSPPTFGGVCC
                     DDSEIFLCEGGQGGRGHTGLPMTFGPESDEALERAVWMSMWVDDVDAVHRRCLEQGIE
                     ITWPPTNMPWGARELHVRHPDGHVFRVSKNIEEEK"
     gene            6962..>7511
                     /locus_tag="VFL84_13690"
     CDS             6962..>7511
                     /locus_tag="VFL84_13690"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HET7609727.1"
                     /translation="MAQAQLVLFVESALRAGQPRDAVRAALEQAGWSKDQIADALAHF
                     ADVAFAVPVPRPRAQLSARDAFWYLLMFGALYTSAFYLGDLLFGFINLAYPDRANASY
                     YANTPEYIERGIRWATAALIVAFPVFMLAALKLGREVAADPTRRNSAMRKWLTYLTLL
                     VAASAIIGDGITLVYNLLSGELT"
BASE COUNT         1323 a         2308 c         2494 g         1386 t
ORIGIN      
        1 cggactacga caagcttgtc ggctaacaag ctctacggag ttccccgtca aggtgggcga
       61 cgatggctct ggatgttttc ggtggcgcgc gagcggttgc gcgacgcgat cggaagcggc
      121 aaggtcgagg cgaagacggc atctctctac gcggccttgt ggggcgagcg ctgaactcgt
      181 ccgggccaag ataaaagacg aacgtggatg gtgtcgatgt cgatgtgacg ctgttcgttg
      241 agggcgtgaa gtcgcattat ccttgcgtga ccggagagct gctggcattt ggccgggagc
      301 tacttcgttt cgctttggcc gccgttgaga atgcacgttg agtttcccgc gtgtcgccgc
      361 acaacaagca gttgcagtgg agccgttcca tttggcgcgt tttctggcgc ttccatcagc
      421 aacgtgcctc attagttttt cctcggcttc cgtgtcggct gacaagcagt caagcagttg
      481 catcggaccg tgagctccgc acaccgttat ccgatcaggg agagtcgttt tgtcgacgcc
      541 taaccagacc gttcccggcg ctgcgcgaat cacggtgcgc tccactgcgg acattgcgag
      601 cgcgttgaaa ggaagcctgt cgcctgaagg cttggtgcta gcagaggagg acttgggccc
      661 agacttctac gacctgaaaa cggggttagc gggagacctt tttcagaaag tggctaccta
      721 ccgaggtagg ctagccatag tgatccgcga cccacacgtc tacggagacc ggttcagtga
      781 gctcgcctac gaacatcgcc gtcatccgtc ggttcgattc ttcgaagagc gcgcgccggc
      841 ggtgcagtgg ttgaacggag gcgcgcgtcg cgacgagcgc actgagtgag ctgcggcgtt
      901 acgcggctga gctgcggcgc tattcgggct aggtcgagct cctatgcatc tttcggcgaa
      961 cgttttcgac gtctgggtct tccgccgccg cggcgcagaa gctgagtttc tgttgctgta
     1021 cacgtccgtt gagaaggcta cgcgccactt caacggaggg cggttttggc agattccaag
     1081 caatgtcgtg aacgacgggg aatcgatagt agaagcgatc cgccgagtgt tggatcgcta
     1141 cggccttcgc ccgaaagcaa tctgggcggg ggagcatgcg tatctgatct acaaccggcg
     1201 ttttgctgaa atgcaagcca tcggcgtgta cgcagcggaa gtcgaggacg ccgctgtcca
     1261 tctcgaccca gccgagcatt ccgagtttgc atggctgtcc cgcgaggatt gcctcaatcg
     1321 agtgcactat cgcggcctca aggacggact acggtccgtg cacgagtacg tcacgggagt
     1381 cgaggcgcct gcgaaagagc tgtgtctcta tatcgcgccg agcgcatgac aaccggttgc
     1441 ggcggatggt gatggcatca cgggcgcgag gaatagcgtg acagtcaagc gcatggacaa
     1501 cgtcggcatc gtcgtggaaa acctcgatgc cgccatcgag ttcttcaccg agctcgggct
     1561 gacgctcgaa ggccggatgc ccatcgaagg cgaatgggcc ggccgcgtca ccggagtgcg
     1621 cggccagcgc gtcgagatcg ccatgctgcg cacgccggac ggccacaacc gcatcgagct
     1681 ttcacgcttc gacgcgcccg cgatcgcgtc cgatcaccgc acggcccccg tgaactcgct
     1741 gggatacctt cgcgcgatgt tcaccgtcga cgacatcgac gaaacgctcg tccggctcga
     1801 gaagcttggc gctaaggtgg tcgatgaagt cgtcgactac gaagcgatct atcggctttg
     1861 ctatatccga ggtcccgaag gaattttgat cgggctcgcc cagcagctcg ggcagcaagc
     1921 ctcaagggag aatcccatgg aacgcaagcg ttgagggtcc tgtgcgagat gtcgccatgc
     1981 tttccattcg tcgtgcaact gcaaacgaca cgggcgaaat cgagcgcatg gtggccgatt
     2041 tcgtcaagga ccaccccgcc gccaaccatc cgcgagatgc agccgcgttg cgcgccgcct
     2101 actttggcga gcatccggtt gcgcagctgc tcgtagccga gcgaaacggc gagatcgtgg
     2161 ggatggcgca gtggcgcctc atctacgata tgttctgggg tatgttcggt gcgcaagctg
     2221 aatggctcta cgtcaagccg cagtgccgcg ggagcggtat cgtcgctgcg ctcgtcgcgc
     2281 gcgtgtgcgc ggaggcttcg gcggcgggag ccaaattcgc gcagggcgga ggcggcgagg
     2341 ggccttcgag actttatgaa cgcgtggcta ttggcatgtc cgatcgggcc tgccacctgt
     2401 ccggcaaggc gttccaggtg tttgcgaagc tcgacggcct gcctgttcgc gaaatcgtcc
     2461 gccgcctacc ggccaaagag ctcgggctgc aaccgccgga ttagcaagtt ccaatatgct
     2521 cggagaggac ggcgcgaagc gtcattgatg tgctgaaaaa tcgcccttgc cccgtatgcg
     2581 ccgaaccgac ggtgaatcct ttcgcgtcat tgatttggcc gcgctgcaaa gtgtgccgct
     2641 ccaagttcaa gctccggctg cccatcggca tcggcttcat tggcgactac gtagggcact
     2701 gcgtgttgct gggctccatt gcggtagctg ttttcgtcgc cgacacttgg gccttcgtag
     2761 tcggcttagc cctcttcacg gccctcgaag tcgcgatctt ccgcgtgagc cggttggagc
     2821 ccgacactcg agaccctgtc acggtcatgc agctcaggcg atactcgcgc gcctaagtac
     2881 gttgaaagct caatgacgat cgagaccgaa cgcgacgttg acggattgcg acatcgagag
     2941 cgagatgaca accgctagac cattcctgat gttccaaggc ggcgttgcgc aagccgcact
     3001 ggatctctat ttcgccacgt tcccggactc tcgcatggtc cgcgtcgacc gctacgcgaa
     3061 gggcgagccc gctcccgaag gcacgatcaa ggtcgccgtc ttcaccattt gcgggcgcga
     3121 gtggatgtgc tcggacagcc cgatcgatca tgcgttcacg ttcacgccgt ctagctcgac
     3181 gttcgtcgat ttcgattcgg cggcggagct cgatcgggtg ttcggcattc tcgtggacgg
     3241 cggcaaggtg ctgatgccgt tgaatgacta cggtttcagc cggcggttcg gctggttggt
     3301 cgatcggttc ggcgtgtcgt ggcagttgaa cttgccggcg tgaccgcgct cgacatccgc
     3361 aagctggcca acgcgttcat tcccgcggcg acaccgtccg accggctgcc gagcgcggcg
     3421 ctgacagccg tcacgcggtt caagcgcaag catggcggcc tgtgggtcgg cggcaccgtc
     3481 gccgccacgg atcgcggcgt gtcgttcgag ccgaacggtc tcaaccgggc cttccacgaa
     3541 gacttggaga cgattagcgt acgtgctgaa gacatccgtg ccgtgcggca cgagttcggc
     3601 tggttcacag gtgtagtggt agtcacgcac gcgggcggcg agtttcgctt ccgttgctac
     3661 ggcgcaaaga agctcgccgc agcgcttgct gcggcttatc gtgagcggcg gagtcatgcc
     3721 caaacataaa gcgcgccgtg tcgcgctcga cctgcgcggc ttgagcgggc gcgaggagct
     3781 ttttcgttta tcgggggccg taaagcgcga tcccgccgtc gaacgttggc tcacgagcgg
     3841 cgcgagcgag ctgcgttcgc ttgcgcagcg ctggttcgcg cgcatgcgcg agtgcggcgc
     3901 cgacgtgcgc gagctaatgc acgatggttg tcccacggct tgcgtcgaag gcgccgcgtt
     3961 cgcgtacgtg agcacgttca aggcgcacgt caacgtcggc tttttctgcg gcgcgcagct
     4021 gcccgacccc gccggcctgc tcgaaggcag cggcaaacgc atgcggcacg tcaagctgag
     4081 gcccggcgag gagccgaacg cggccgcgct cgacgagctc atcgacgcag cgtaccgcgc
     4141 catcaaggca cgcctcgccg ccgagcgcgc cgcggccgat gactgacacg gacgccgagc
     4201 gctacgagat ccgccgcgcg cgatcttgga atctgtccag gcgcggctcg gctgggccga
     4261 gcccgacgtc gcgccgctcg ggtagtggcg gggctcgggc gaacttttcg agcttcgtgt
     4321 aaccaaatcc ggggctcgcg tatctaatgc gacagggaac gggagaggtt cccgaagacc
     4381 gcgctcatgc cgcaataccg attcaacgtg ttcggacgtc tcgtcgtgat caaaggcgcg
     4441 cccggcaatt ggtcggcgtt cttgctcggc gcggacggca agcggcgcaa ggccgacttc
     4501 ctggtaccgg gctcgctgtg cgaggacgag ctctgcggct acctgtccga tttgttccac
     4561 gagtcggcga gctcgacgca gcccgccgtc acgcgcctcg actgctcgtc gaatacctga
     4621 cacaggccgt caggtggcac gtgtaagctc atattgacgg gcaaacggag caccgccatg
     4681 gcgagcactc accacgcgat cgactacatc gagttcacgg tccgcgacct gaatgccgcg
     4741 aagcgcttct acgcggcggc gttcggctgg cagttcaacg actacgggcc cgagtacgcc
     4801 ggcatcaaag gcgtcgacgg cgaggtcggc gggctccagc aagcggcgca gccgcgcacg
     4861 ggcggaccgc tcgtcgttct ctattcgaaa gacctcgaga agacgctcgc cgcggttcgt
     4921 gcggccggcg gcaagatcgt gcgcgagccg ttcgcgttcc cgggcgggcg gcgctttcag
     4981 ttcacggacc cgagcggcaa cgagctcggc gtctggtcgc aagcccagga ccactgaagc
     5041 gacgcgattt ttgccaggat cgagcgggcg gcgtactgtt tctgccgtga atctcgcagc
     5101 cgtgcgtcgc ttggtcgaac agcgcgagat catggtccgc gtcgatccga aggcctacga
     5161 gacactgacc tagggcaaga tcgcgtacct gcatgtgcac ctgacgaacg cgaaggcgcg
     5221 cgacgtcgag acgttgttgc gctcggcgtg ggaacgcaag gcgccgaaac ggctcgtcgc
     5281 gccgtcgggc tgagaatcga gatgacagtc agatgcgact gagtgcttta gcgatcagcg
     5341 gcgcggcggt cgcgctcgcg atcgcgctcg tggcctactt tcgacctggc ggcgagcccc
     5401 cgctcgtttc gaagagcgag acgccgcctg tgcaggaggt tcccgtcgcg aacgagccgg
     5461 cggccgcgcc tcctctggaa gcgaccgtct ttgtgccgcc tgcggccgag tcgcgtgcgg
     5521 gcgcaggtcc tgtgccgagc atcgagcctg cgccgctgcc gggtgcaacg gcgacgacgc
     5581 cgatggccga cctcatggcg ggaagaggaa cagatcgacc gatgcccgcg ctcatcgaac
     5641 gcgagcgggc attcgcggta gaacccgtcg accggacgtg ggcgccagcc gccgaggcgc
     5701 gcatatacgc gaagctcgcg gagataccgg ggctcaagct catcggtttg caggtcgaat
     5761 gccgatcgac gatgtgccgt atccagaccg agtcgagcgg cgggtcctcg ctgcccttca
     5821 aggatttgct cgggccggcc ggtctcgagc cggcctggat ggcaacgatc aaggagcgca
     5881 acggtccagt caggtccgcc gcgtatctgt ggcgcgatgg cttcgcgccc ccgaccgagc
     5941 cgcttcaacc gcgcgacacc aactgacatc gattgcgaag gcttgtcttc gcatcgcggc
     6001 ggcagcacgc tgtaaccgaa aaaagaaggg ggaagcttgg cgatcgacgt gcgcggtgtc
     6061 tgcacactgg tgctcgtgtt cgatatgccg acgtcgctga agttttaccg cgacgtgctc
     6121 ggcttcgagg tcgtcgcaac gaacgacgac agcgccggcg acaacgtcga ttggtgctac
     6181 ctgcggctcg gcaaggccga gctgatgctg aacggcatgt acgaagcgca tcggcgccct
     6241 gcggcacccg acaagaaacg catcgcgaca cactccgacg tctgtctcta tttcgcctgc
     6301 cccgacgtcg acggcgcgta ctgctacctg cgatcgaaag gcgtcgatgt gaaggaaccg
     6361 caggtggcgc gttacggtat gaagcagctc taccttctcg atcccgatgg ctacaacctc
     6421 tgcttccagt gggaagcgac gcccgtggag cagccggtgc catgaaagtt catcgcctga
     6481 ctccgatcct gaacgtctcg gatatccagg aaagcttcga gtggttcgag aagctcggct
     6541 ggaaaaaggc ctgggactgg ggctcgcccc cgacgttcgg cggcgtctgc tgcgacgaca
     6601 gcgagatttt cctgtgcgaa ggcggacaag gcgggcgcgg gcacaccggc ctgccgatga
     6661 cgttcggccc ggagtcggac gaggcgctcg agagagccgt ctggatgtcg atgtgggtcg
     6721 acgacgtcga cgccgtgcat cggcgctgcc tcgagcaagg catcgagatc acgtggccgc
     6781 cgacgaacat gccctggggc gcgcgcgagc tgcacgtgcg ccatccggac ggccacgtgt
     6841 tccgcgtcag caagaacata gaagaagaga aatagatctt cgaggacggg gaggagcccg
     6901 agctcgtcgc gcgagatatg caagccttct tcgtctcggt ggcccgttag cggaggtcgg
     6961 catggcgcag gcacagttgg ttctgttcgt cgaatcggcg ctgcgcgcgg gtcagccgcg
     7021 cgacgccgtg cgcgcggcgc tcgagcaggc cggttggtcg aaggaccaaa tcgccgacgc
     7081 gctcgcgcac ttcgcggacg tcgcgttcgc cgtgccggtg ccgcggccgc gcgcgcaact
     7141 gtccgcgcgc gacgcgttct ggtatctgct gatgttcggc gcgctgtaca cgagtgcgtt
     7201 ctatttgggc gatctgctgt tcggtttcat caacctcgcg tatcccgacc gagcgaacgc
     7261 gagctactac gccaacacgc cggagtacat cgaacgcgga attcgctggg ccacggcggc
     7321 tttgatcgtc gcgttcccgg tcttcatgct cgcggctctg aagctggggc gcgaggttgc
     7381 cgctgatccc acccgccgca actccgccat gcgcaagtgg ttgacctacc tcacgctgct
     7441 cgtagccgcg agcgcgatca tcggcgacgg gatcacgctc gtatacaacc tgctgagcgg
     7501 cgagctaacc g
//
LOCUS       DASSNN010000728         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1507553, whole genome shotgun sequence.
ACCESSION   DASSNN010000728 DASSNN010000000
VERSION     DASSNN010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507553"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFL84_13695"
     CDS             <1..185
                     /locus_tag="VFL84_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609728.1"
                     /translation="RFLARHQTELEHVHVLVGSKMVELTVAIARHRSRTDRLIQIYSD
                     PAIFEAQLDAACKRRR"
     gene            complement(194..1174)
                     /locus_tag="VFL84_13700"
     CDS             complement(194..1174)
                     /locus_tag="VFL84_13700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7609729.1"
                     /translation="MAALSILDLAFVPEGGTPADALRRALELAQHAETWGYTRFWLAE
                     HHNMAGVASAATSVVIGFIAGGTRTIRVGAGGIMLPNHSPLVIAEQFGTLESLYPGRI
                     DLGLGRAPGTDQRTLRALRRDPLSAESFPQDVLELQALLAPRKPGQVVEAVPGTGLEV
                     PLWILGSSLYGAQLAAALGLPYAFASHFAPDELMGALATYRAGFRPSAQLAAPYAMVG
                     ANVVVAETDAEARRLFTSVQQGITNLLRGARGRLQPPIDDIESYWTPPEKAQASRMLA
                     CSFVGSPATVRAGLTRFVAETGADELIVAAGIYDQAARLRSYELLAEALR"
     gene            complement(1184..1828)
                     /locus_tag="VFL84_13705"
     CDS             complement(1184..1828)
                     /locus_tag="VFL84_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET7609730.1"
                     /translation="MNAVLHYIYDPLCGWCYGAEPLVSAASNVDGLALRMHAGGLWPQ
                     PTRLPDHMRRYIQQADARVGQMSGQPYGEPYLNGLLFDPDLVLESRPVIAAVLAAQAL
                     DPGKALPMLRAIQHAHYERGQHVVREATLLAIAAEIGLDSAAFAEAFKTVRVDEHIAE
                     SQELMNNVGAQGFPTFVLQIGDEWFAVPHGRFASAPGKFAEWLDAQLRAQSRTH"
     gene            1916..>2114
                     /locus_tag="VFL84_13710"
     CDS             1916..>2114
                     /locus_tag="VFL84_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HET7609731.1"
                     /translation="MNAPPLDPLASPCVRNCCLDDDNVCMGCGRALQEIVAWGTASDA
                     DKTAILARSRERLRTRAIRAAK"
BASE COUNT          333 a          744 c          743 g          294 t
ORIGIN      
        1 cccgcttctt agcgcgtcat cagaccgagc tcgagcacgt gcacgtgctc gtcgggtcga
       61 agatggtcga gctcacggtc gcgatcgcgc gccaccggtc gcgcacggat cgcctgatac
      121 aaatctattc cgacccggcc atcttcgagg cgcagctcga tgccgcctgc aagcgccggc
      181 ggtgagcgcc cgatcagcgc aacgcttcgg cgagcagctc gtacgagcgg agccgcgcgg
      241 cttgatcgta gatgcccgcc gcgacgatga gctcgtcggc acccgtctct gcgacgaacc
      301 gcgtgagtcc cgcgcgcacc gtcgccggcg agccgacgaa cgagcacgcg agcatgcgcg
      361 aggcctgcgc cttctcgggc ggcgtccagt agctttcgat gtcgtcgatc ggcggctgca
      421 gccgcccgcg cgcgccgcgc agcaggttcg tgatgccttg ctgcaccgac gtgaagagcc
      481 gccgcgcctc cgcatccgtc tcggccacga cgacgttcgc gccgaccatc gcgtacggtg
      541 cggcgagctg cgccgacggc cgaaagcccg cgcgatacgt cgcgagcgcg cccatgagct
      601 cgtccggagc gaaatgcgat gcgaacgcgt acggcagccc gagcgccgcc gcaagctgcg
      661 cgccgtacaa gctcgagccc aggatccaga gcggcacctc gaggcccgtg cccggcacgg
      721 cctcgacgac ttggcccggc ttgcgcggcg cgagcaacgc ctgcagctcg agcacgtcct
      781 gcggaaacga ctcggcgctg agcggatcgc gccgcaatgc ccgcagcgtg cgctggtccg
      841 tgcccggcgc gcgcccgagc ccgagatcga tgcggcccgg gtacagcgac tcgagcgtgc
      901 cgaactgttc cgcgatcacg agcggcgagt ggttcggcag catgatgccg ccggcgccga
      961 cgcgaatcgt gcgcgtgccg cccgcgatga agccgatgac gaccgacgtc gcggcgctcg
     1021 cgacgccggc catgttgtgg tgctcggcga gccagaagcg cgtgtagccc caagtctcgg
     1081 catgctgcgc gagctcgagc gcgcggcgca gcgcgtcggc cggcgtgccg ccttccggca
     1141 cgaacgccaa atcgaggatc gacaacgcgg ccatggtggc gcgctagtgc gttcgcgact
     1201 gcgcgcgcag ctgcgcgtcg agccactcgg cgaacttgcc cggcgcggac gcgaatcgcc
     1261 cgtgcgggac ggcgaaccat tcgtcgccga tctggagcac gaacgtcggg aacccctgcg
     1321 cgccgacatt gttcatgagc tcctgcgatt ccgcgatgtg ctcgtcgacg cggaccgtct
     1381 tgaaagcctc ggcgaacgca gcggagtcga gcccgatctc ggcggcgatc gcgagcaaag
     1441 tcgcttcgcg gacgacgtgc tggccgcgct cgtaatgcgc gtgctgaatc gcacgcagca
     1501 tcggcagcgc cttgccggga tcgagcgctt gcgcggccag cacggccgcg atgacgggcc
     1561 gcgactcgag cacgagatcg ggatcgaaca agaggccgtt cagatacggc tcgccgtacg
     1621 gttggccgct catctgcccg acgcgcgcat cggcctgttg gatgtagcgg cgcatgtggt
     1681 cgggcagccg cgtcggctgc ggccacaagc cgccggcgtg catgcgcagc gcgagcccgt
     1741 cgacgttcga cgcggcggag acgagcggct cggcgccgta gcaccagccg cagagcggat
     1801 cgtagatgta gtgcaggacg gcgttcatgc gccatctaac accggcgaat gcgcagggcg
     1861 caagcgagcg ccggttcacg ccgaccggcg agcgagtggc ttatcatcgc gacgcatgaa
     1921 cgcgccgcct ttggatccgc tcgcctcgcc gtgcgtgcgc aactgttgtc tcgatgacga
     1981 caatgtctgc atgggctgcg gccgcgcgct gcaggagatc gtcgcctggg gcacggcgag
     2041 cgacgcggac aagaccgcga tcctcgcgcg cagccgcgag cgcctgcgca cgcgcgctat
     2101 tcgcgcggcg aagc
//
LOCUS       DASSNN010000729         3853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_498444, whole genome shotgun sequence.
ACCESSION   DASSNN010000729 DASSNN010000000
VERSION     DASSNN010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498444"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..2554
                     /locus_tag="VFL84_13715"
     CDS             98..2554
                     /locus_tag="VFL84_13715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7609732.1"
                     /translation="MSAAGLNRLEEARRALRHAWRGLLKSPWFTGLAVLTLGLGIGVN
                     TGVFSVIYSTLIAPLPYDDAGRLVLIRQSSPLAGQGDVGVSIRELYDYRERLRSFDEI
                     VEFHQMDFDLLRRGEPDRVATGVVSHNFFDVLGIRPLHGRTFVEADDRAGAEAVLVLG
                     HAYWQNRFGGDPNVIGQVLEMNDRPHTVIGVLPAVALYPNEVDVFMPTSACPFRAAGE
                     LQMEANRRAFSILRVFGKLKTGVYPSTAAAEVAAVGNDFAREFPSIYRPEAGFRAQTL
                     GVQDALTQNARPMLLVLLGITGLVLLLACANVGNLTLARTLQRSHERATRAALGAGRL
                     SLLAQPLAESTLVAIAGAVVGLAFAWATLGLLATFIGRFTSRAGEIGISWPVFAFTLG
                     IALVAGVLLGVLPALGAPVDVAGAIKTGSKGGGGPRRLLQHVLVAGQVTVSVVLLAAA
                     GLLLLSFVRLQSVDAGYRVDHVLSAEIFANFSKYATPESRQRLYEDVLERLQAVPGIT
                     SAAITNAVPLSGMRPGQTRLRIEGAGDAGELAPTVDGRIASPRYFETLGIPLIRGRVF
                     EDTDTATTERVAVINETAARQWGELDPFGARVAIDGGRNWYRIVGIVGDVRNFGLDVE
                     AVAQAYTPLAQANGNIQGRILARTAGDPGAAMGAIRAAIQSVDPDMPIERMQTLDTIR
                     SAYLATPRLTALLLSLFAGLALCITVTGIAGVIATSVAQRTREIGLRIAVGASSRGVL
                     AMVLKQGFKLVGIGLCVGVPLAVLFGLALRSYLFATPPADPLMLAGVALALAAAGAIG
                     CAGPALRATRIDPISALRAD"
     gene            2724..>3853
                     /locus_tag="VFL84_13720"
     CDS             2724..>3853
                     /locus_tag="VFL84_13720"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET7609733.1"
                     /translation="MRTFRLRVMTALFAALALGLLEEAKVLAQNPAPNAGDDSLEEIV
                     VTGSRIARRDFTSQSPIVTIDQSTLTTRTNVGMEAALNQFPQFTPLGTQAQNSPAGTP
                     FPQADAAPGAATLNLRGLGGNRSLVLVDGRRVQPVNGQLLVDINTIPSAAISRVEVIT
                     GGAAAVYGADAIAGVVNFITKKDFTGVEVNAQSSITAEGDGEETSISGMLGAALPDGR
                     GSVMLGADWSKRNIIFGRDRDWVVKGWRDPGTTAGGIGSSNLSQFDPARASYCGVTNC
                     GQPLGPPFGAGNAPTGGFPLTGGTIYGIDQNGNLFDPNNPLNPAHPYTGPLGGDSNFK
                     LNPAQTPGTPQTLGYFDPEHSYLQLPLERYAIFGSGAIDLTE"
BASE COUNT          603 a         1316 c         1274 g          660 t
ORIGIN      
        1 cgtccaaata cgcaaggaag acaatattcg tgctggtcaa ttcgagctaa tttggacagc
       61 agtgatctgt ccccttttcc ggcagggggt gaagccgatg agcgctgcgg gactcaaccg
      121 actcgaagag gcacggcgcg cgctgcgcca tgcgtggcgc gggctcctca agagcccgtg
      181 gttcacgggc ctagcggtgc tcacgctcgg cctcggcatc ggcgtcaaca cgggcgtttt
      241 cagcgtgatc tattcgactc tgatcgcgcc gctgccgtac gacgacgcgg gccggctcgt
      301 gctgatccgc cagtcctcgc cgctcgcggg tcagggcgac gtcggcgtct cgattcgcga
      361 gctctacgac taccgcgaac ggctcaggag cttcgacgag atcgtcgagt tccaccagat
      421 ggacttcgac ctgctgcgcc gcggcgagcc cgatcgcgtc gcgacgggcg tcgtgtcgca
      481 caacttcttc gacgtactcg gcattcggcc gctccacggc cggacgttcg tggaggccga
      541 cgaccgcgcc ggcgcggagg ccgtgctcgt gctcgggcac gcgtactggc agaaccgctt
      601 cggcggcgac cccaacgtga tcggccaggt gctcgagatg aacgaccggc cgcacaccgt
      661 gatcggcgtg ctgccggccg tagcgctcta tcccaacgaa gtcgatgtct tcatgccgac
      721 gtcggcgtgc ccgtttcgcg ccgcaggcga gctccagatg gaagccaatc ggcgcgcgtt
      781 ctcgatcttg cgcgtgttcg ggaagctgaa gaccggcgtg tatccttcga ccgcggcagc
      841 cgaagtcgcg gccgtcggca acgacttcgc gcgcgagttc ccgagcatct atcgccccga
      901 ggccggcttt cgcgcgcaaa cgctcggcgt gcaagacgcg ctgacgcaga acgcgcggcc
      961 gatgctgctg gtgctacttg gcatcaccgg gctcgtgctg ctgctcgcat gcgcgaacgt
     1021 cggcaacctc acgctcgcgc gcacgttgca gcgctcgcac gagcgcgcca cgcgcgcggc
     1081 gctcggcgcc gggcgtttga gcttgctcgc acagccgctc gccgagagca cgctcgtcgc
     1141 gatcgcgggg gccgtcgtcg ggctcgcgtt cgcatgggcg actctcggtt tgcttgccac
     1201 gttcatcggc cggttcacgt cgcgcgccgg ggaaatcggc atctcttggc ccgtgttcgc
     1261 gttcacgctg ggaatcgcgc tcgtcgccgg cgtgctgctc ggcgtgctgc cggcgctcgg
     1321 agcacccgtc gacgtcgcgg gcgcgatcaa gacgggatcg aaaggcggcg gagggccgcg
     1381 ccggctcttg cagcacgtgc tcgtcgcggg ccaggtgacg gtctccgtcg tgttgctcgc
     1441 ggccgccggc ttgttgctgc tcagcttcgt ccggctacag agcgtcgacg cggggtatcg
     1501 ggtcgaccac gtgctctcgg ccgagatctt tgcgaatttt tcgaaatacg cgacgcccga
     1561 gtcgcggcag cggctgtacg aggacgtcct cgagcgtttg caggccgtgc ccggcatcac
     1621 ttccgccgcc atcacgaacg ccgtgccgct atcgggcatg cggccggggc agactcgatt
     1681 gcggatcgag ggcgccggcg acgcgggcga gctcgcaccg acagtcgacg ggcgcattgc
     1741 gtctccccgg tatttcgaga cgctcggcat cccgttgatc cgcggccgcg tgttcgagga
     1801 caccgacacg gcgacgacgg aacgcgtggc cgtcatcaac gaaaccgccg cgcgtcaatg
     1861 gggtgagctc gaccccttcg gcgctcgggt ggcgatcgac ggcgggcgca actggtaccg
     1921 cattgtcggc atcgtaggcg acgtgcggaa cttcggcttg gacgtcgaag ccgttgcgca
     1981 agcctatacg ccgctcgcac aggcgaacgg caacatccaa ggccgcatcc tcgcgcgcac
     2041 ggccggcgat cccggcgccg ccatgggcgc gatccgcgcc gcaattcaga gcgtagatcc
     2101 cgacatgccg atcgagcgca tgcagacgct cgacacgatt cgcagcgctt atctcgccac
     2161 gccgcgcctc acggcgctgc tgctgtcgct gttcgcgggc ctcgcgctct gcatcacggt
     2221 gaccggcatt gcgggcgtca tcgcgacgtc ggttgcgcag cgcacgcgcg agatcggcct
     2281 caggatcgcc gtcggcgcga gctcgcgcgg cgtgctcgcg atggtgctga agcaaggctt
     2341 caagctcgtc ggcatcgggt tgtgcgtagg cgtgccgctc gccgtgctgt tcgggctcgc
     2401 gctgcgctcg tatctgttcg cgaccccgcc ggccgatccg ctcatgctcg ccggcgttgc
     2461 gctcgcgctc gcggcagcgg gcgccatcgg ctgcgccggc cccgcgctac gcgccactcg
     2521 catcgatccg atctcggcgc tgcgagccga ctagacgctc ggtacggtag cgaggtcagc
     2581 ggccggcgga ggccttgcgc catgggctcg caggtgattg gaattttgcc tcatctgtga
     2641 gacaaatatc caaagtgact cgcgagacaa atatatccgc gtgtctcttc tgcagcgtcg
     2701 gttcattcct ttggggggtt gccatgcgaa cgttccgatt acgcgtcatg acggcgttgt
     2761 ttgcggcatt agcgctgggt ctgctcgagg aagccaaggt gcttgcgcaa aacccggcac
     2821 ccaacgcggg cgatgattca ctcgaggaaa tcgtagtcac cggatcgcgc atcgcccggc
     2881 gcgacttcac gtcgcagagc ccgatcgtga cgatcgatca gtcgacattg acgacgcgga
     2941 ccaacgtcgg catggaggcc gcgctgaacc aatttcctca attcacgccg ctcggcacac
     3001 aggcgcagaa cagcccggcc ggcacaccgt tcccgcaagc cgatgccgcg cccggcgccg
     3061 ccacgctgaa cctgcgcggc ctaggcggca accgcagcct cgtgctcgtc gatggccggc
     3121 gagtgcaacc cgtcaacgga cagctgctcg tcgacatcaa cacgatcccg tcggcggcga
     3181 tctcgcgtgt cgaggtcatc accggcggtg cggcggccgt gtacggcgcc gacgcgattg
     3241 ccggcgtcgt caacttcatt acgaagaagg acttcacggg cgtcgaggtc aatgcccagt
     3301 ccagcatcac ggccgagggc gacggcgagg aaacctcgat tagtggcatg ctcggcgcgg
     3361 cgctgcccga tggccgcgga tcggtcatgc tcggggccga ctggagcaag cgcaacatca
     3421 tcttcggccg agatcgcgac tgggtcgtga aaggatggcg cgatcccggc acgacagccg
     3481 gcggcatcgg cagctccaac ctgtcgcaat tcgatcccgc gcgcgcgtcc tactgcggcg
     3541 tcacgaattg cggacagcct cttgggccgc ctttcggcgc gggcaatgcg cccacaggcg
     3601 ggttcccgct cacgggcggc acgatctacg gcatcgatca gaacggcaac ctcttcgatc
     3661 ccaacaaccc gctgaatccg gcccacccct ataccgggcc gctcggcggc gactcgaact
     3721 tcaagctgaa tccggcccag actccgggca cgccgcagac gctcgggtat ttcgatccgg
     3781 agcacagcta cttgcaactg ccgctcgagc gctacgcgat cttcggctcg ggcgccatcg
     3841 atctcaccga gaa
//
LOCUS       DASSNN010000730         6053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1871660, whole genome shotgun sequence.
ACCESSION   DASSNN010000730 DASSNN010000000
VERSION     DASSNN010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871660"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1818
                     /locus_tag="VFL84_13725"
     CDS             52..1818
                     /locus_tag="VFL84_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HET7609734.1"
                     /translation="MLFALAFLGGVLTIVSPCILPVLPFVLTRTNVPFRRGALPLLAG
                     MALTFAAVASLAAVGGGFVVSANRWGRWAALSLMLVFGLALLLPSFAQRLGAPLVALG
                     HRLSGAAARRDGRAGSSLLLGVATGLLWAPCAGPILGLILTGAALNGANLETSLLLLA
                     FAAGSAASLAAALLLGARVFAALKRSLGAEEWLRRALGAAVVVSAVAIAFGLDTGFLA
                     RVSTASTTRIEEAVFGRFMPARDALVSGDGAMMAGGGAMMSARGTAGALPVEGVMPPL
                     DGVTQWLGSEPLTRESLRGKVVLVDFWTYSCINCIRALPFVKAWHEKYRDRGLVVIGV
                     HTPEFAFEKDLGNVRRAVEELGLTYPVAVDNDYAVWRAFDNRYWPAHYFIDAAGNIRH
                     EHFGEGEYEASERVIQRLLAEAGGAGVATDLVAPNAEGAALASDLLSVVTPETYLGHA
                     RAEHFASPGGQIRGAAHEYAAPQRLGLNEWALAARWTVGEEAAVLNGAPGKIVMRFQA
                     RDLHLVLGPGPDGKPVRFRVTLDGRAPGADHGADVGADGGGTVDGYRLYQLIRQQGAV
                     RERIFEIEFLGSGVAAYAFTFG"
     gene            1829..2536
                     /gene="msrA"
                     /locus_tag="VFL84_13730"
     CDS             1829..2536
                     /gene="msrA"
                     /locus_tag="VFL84_13730"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET7609735.1"
                     /translation="MRSQASIWSRAAAVLALVALHGLAHAAEQAVLIPAPAFDEQLAD
                     ASSSATAVLAGGCFWGVQGVFQHVEGVLKAVSGYAGGSAANAKYELVGNGDTGHAETV
                     RIVYDPSKITYGRLLQIFFSVVHDPTQLNKQGPDHGPPYRSAIFVENAQQRAIAERYI
                     AQLEAAKVYRAKIVTTLEEAPFYPAEAYHQDFLERNPTHPYIAYNDLPKVRDLRSVFP
                     NVYRAEPALVLARARAR"
     gene            complement(2827..5784)
                     /locus_tag="VFL84_13735"
     CDS             complement(2827..5784)
                     /locus_tag="VFL84_13735"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7609736.1"
                     /translation="MDDRTTSFGYWLKRRRKALDLTQEQLAQGVACSHFAIRKIEADE
                     RRPSRRLAERLADRLGIPPHERGAFLEAARALRSVDRLAVDNTPLTPNRVEQAEAGPF
                     VGRGKEIDLLAGLLARLGPGVGRVALVEGEPGIGKSRLTRELARQARARGAATLATKC
                     YEIEAAMPYQAVIDVVTQALELMPTAVLQKLPPVSLAEIAALVPGVALRAAVPVLSAD
                     LPEARQARIFHALVQLLELLTATRHLLVIVDDLQWADDASAQFLHYLARRTAALPMLT
                     VYAYRDEELDTRAGLAELVASVRGEAHVHHMTLARLELAETKELLASQGADLSERLAV
                     RLHRETDGNPFFLTSMLQAMGAGEIAAHADAELPLPDALRASVRARLAHLPDDARPAL
                     DVAAVLGRRFDFESLFAVMKTSEDELLHAVETLVRWRVLREESAGTYDFSHDKVREVA
                     YLEIGGAGRRLLHKAVAEALEQQDEGEAHERDARLAEHYERGHVWGKALHHMVLVAQR
                     SLKLFAVREALGWFDRAIALASAHRGTITERALVDLHEQRGRARAQAGQTDGAVADTR
                     IVIDAARARGDRTKVREALIQLGMTYRRGDDYGAAAECLGQALAECRTLGDERQVADT
                     LYHLGTVMWSNGHNAEAIAHHAEAVALCERHGLSDLVAVQAYHGRGEAHFFNLEHAAA
                     IRCYERSIELARGIGDKSYESENLMMIGHAYLGHLGIGDHAQARVYFEAALEIAERAD
                     LQWHIPPTLLGLACVRGYLGDYGGAVSDIRQTIGSIEPLKLPRYQIMAYEMLASLLLD
                     VGLNRRALEASERGIAIAGSVQTTFWRGRCEGTLAMARMRLADLDVEPALASTLRWAR
                     ENDERSQLVRCLEALAELELRRGDLDACGAFADEMLALGNGAGMKELTACGHWWRGEA
                     LAASGKRDAAIEHLSLAASGAEKLGRVRLAKDALEALVKVSGDARHVARASALAARIA
                     DSARECERLMSGS"
BASE COUNT          852 a         2114 c         2098 g          989 t
ORIGIN      
        1 cgctactgca tcaacggcct cgccctgaag ttcgtgccgg cctagctcgc catgctgttc
       61 gcgctcgcgt ttctcggcgg cgtgctcacg atcgtgagcc cgtgcatcct gcccgtgctg
      121 ccgttcgtgc tcacgcgcac gaacgtgccg ttcaggcgcg gggcgttgcc gttgctcgcg
      181 ggcatggcgc tgacgttcgc ggccgtcgcc tcgctggcgg ccgtcggcgg cggcttcgtc
      241 gtcagcgcga atcggtgggg acgctgggcg gcgctgtcgt tgatgctcgt gttcgggcta
      301 gcgctgctgc tgccgagctt tgcccagcgc ctcggcgcgc cgctcgtcgc gcttgggcat
      361 aggctctcgg gcgcggccgc gcggcgcgac ggccgcgccg ggtcgtcgtt gctgctcggc
      421 gtcgcgacgg ggctgctctg ggcgccgtgc gcgggcccga tcctcgggct gatcctgacg
      481 ggcgcggcgt tgaacggcgc aaatctcgag acctcgctgt tgctgctcgc gttcgccgcg
      541 ggcagtgccg cgtcgctggc cgcggcgctg ctcctcggag cccgagtgtt cgccgcgctg
      601 aagcgctcgc tcggcgcgga ggagtggctg cgccgcgcgc tcggtgccgc cgtcgtcgtc
      661 agcgcggtcg cgatcgcatt cgggctcgac acgggcttcc tggcgcgcgt gtcgacggcc
      721 agcacgacgc ggatcgagga ggcggtgttc ggccgcttca tgcccgcgcg cgacgcgctc
      781 gtgtccggcg acggcgcgat gatggcgggc ggcggcgcga tgatgagcgc gcgcggcacg
      841 gcgggcgcgt tgcccgtcga gggcgtcatg ccgccgctcg acggcgtgac gcagtggctc
      901 ggctccgagc cgctgacgcg cgagagcttg cgcggcaagg tcgtgctcgt cgacttctgg
      961 acttactcgt gcatcaactg tatacgcgcg ctgccgttcg tgaaggcgtg gcacgagaag
     1021 tatcgtgacc ggggcctcgt cgtgatcggc gtgcacacgc ccgagttcgc gttcgagaaa
     1081 gacctcggca acgtgcgccg cgccgtcgag gagctcggcc ttacgtatcc cgtggccgtc
     1141 gacaacgact acgccgtctg gcgcgcgttc gacaatcggt actggcccgc gcactacttc
     1201 atcgacgcgg ccggcaacat tcgccacgag cacttcggcg aaggcgagta cgaggcgtcc
     1261 gagcgcgtga tccagcggct actcgccgag gccggcggcg ccggcgtcgc gacggatctc
     1321 gtcgcgccga acgcagaagg cgcggcgctc gcgtccgatc tcctcagtgt cgtgacgcct
     1381 gaaacctact taggccacgc gcgcgccgag catttcgcgt cgcccggcgg gcagatccgc
     1441 ggcgccgcgc acgagtacgc agcgccgcag cggctcggtc tcaacgagtg ggcgctcgct
     1501 gcgcgctgga ccgtcggcga ggaggccgcg gtgttgaacg gcgcgcccgg taagatcgtc
     1561 atgcgctttc aagcgcgcga cctgcacctc gtgctgggcc ccggccctga cggcaagccc
     1621 gtgcggttcc gcgtcacgct cgacgggcgg gcgccgggcg ccgatcatgg cgcggacgtg
     1681 ggcgccgacg gcggcggcac cgtggacggc tatcggctct atcaattgat ccgccagcaa
     1741 ggcgccgtcc gcgagcggat tttcgagatc gaattcttgg gctccggcgt cgcggcgtac
     1801 gccttcactt tcggctagga gattccgtat gcgctcgcaa gcttcgatct ggtctcgcgc
     1861 ggcggccgtg ctcgcgctcg tcgcgctgca cgggctcgcc cacgccgcgg agcaagcggt
     1921 gctgattccg gcgccggcgt tcgacgaaca gctagccgac gcatcgtcgt cggcgaccgc
     1981 cgtgctcgcg ggcggctgct tctggggcgt gcagggcgtg ttccagcacg tcgagggcgt
     2041 gctcaaagcc gtgtcgggtt atgccggcgg ctccgccgcg aacgcgaagt acgagctcgt
     2101 cggcaacggc gacaccggcc acgccgaaac cgtgcgcatc gtctacgacc cgtcgaagat
     2161 cacgtacggg cggctgctgc agatcttttt ctccgtcgtt cacgatccga cgcagttgaa
     2221 caagcagggc cccgaccatg gcccgccgta ccgctcggcg atcttcgtcg agaatgcgca
     2281 gcagcgagcg atcgccgagc gctacatcgc gcagctcgag gccgccaagg tgtaccgggc
     2341 gaagatcgtg acgacgctcg aggaagcgcc gttctaccca gccgaggcgt atcaccagga
     2401 ctttctcgag cgcaacccga cgcacccgta catcgcgtac aacgatctgc cgaaggtgcg
     2461 cgacctgcgc agcgtcttcc cgaacgtcta tcgcgccgag cccgcgctcg tgttggcgcg
     2521 agcacgcgcc cgttgatctt cgtgggcggg tgcgggcgag ctacaagcgc ctcgtccgcc
     2581 ggatggcttc ggcgacgtcg atcatttgac tcagttcttg taatccgaaa tcagaaacgg
     2641 ccttgatcag ctgcgcttcg agatcggcat agacgggatc cgacgattgg ccgacggccg
     2701 agcgatcgct tttaaccata acgcctcccc aggctagctc ccatcgtaga aggtgcaatt
     2761 tgtatgcctc cgcggcgtcg ccgcactgcg gggcatcgtc gcgctccccc ggagacgtga
     2821 gctttctcag ctccccgaca tgagccgctc gcactcgcgc gcgctatcgg cgatgcgtgc
     2881 cgcgagagca gacgcacgag ccacgtgtcg cgcgtcgccg ctgactttga cgagcgcttc
     2941 gagcgcgtcc ttggcaagcc gcactcgtcc gagcttctcg gcgccggagg cggcgagcga
     3001 aagatgctcg atcgcggcat cgcgcttgcc tgaggcggcc agcgcttcgc cgcgccacca
     3061 atggccgcac gccgtcagct ctttcattcc cgcgccgttg ccgagcgcga gcatctcgtc
     3121 ggcgaacgcg ccgcaagcat cgaggtcgcc gcgccgcagc tcgagctcgg ccaacgcctc
     3181 gagacagcga accaactgcg aacgctcgtc gttctcgcgc gcccagcgca gcgtgctcgc
     3241 gagcgcgggc tccacgtcga ggtcggcgag ccgcatgcgc gccatggcga gggtcccctc
     3301 gcaccgcccg cgccagaacg tcgtttgcac ggagccggcg atcgcgatgc cgcgctccga
     3361 cgcctcgagc gcgcggcggt tcaggccgac gtcgagcagc aagctcgcca gcatctcgta
     3421 agccatgatc tggtagcgcg gcagcttcag cggctcgatc gagccgatcg tttgccggat
     3481 gtcgctgacg gcgccgccat agtcgccgag gtacccgcgc acgcaggcga gaccgagcag
     3541 cgtgggcggg atgtgccact gcaggtcggc gcgctccgcg atctcgagcg cggcctcgaa
     3601 gtagacgcgc gcctgcgcat ggtcgccgat gccgagatgc cccaagtacg cgtgcccgat
     3661 catcatgagg ttctcggact cgtagctctt gtcgccgatg ccgcgagcga gctcgatcga
     3721 gcgctcgtaa caacggatcg cggcggcgtg ctcgaggttg aagaaatgcg cctcgccgcg
     3781 gccgtggtag gcctgcacgg cgacgagatc ggaaagaccg tggcgctcgc ataacgcgac
     3841 ggcctcggcg tgatgcgcga tcgcttctgc gttgtggccg ttgctccaca tgaccgttcc
     3901 caagtgatac agcgtgtccg cgacttgccg ctcgtcgccg agcgttcggc actcggcgag
     3961 cgcttggccg aggcattcgg ccgccgcgcc gtagtcgtcg ccgcgccggt acgtcattcc
     4021 gagctgaatc agagcttcac ggaccttcgt tcgatcgccg cgagcgcgcg cggcgtcgat
     4081 gacgatgcgc gtgtcggcga ccgcgccatc cgtctgcccc gcctgcgcgc gcgcccggcc
     4141 gcgctgctcg tgcaagtcga cgagggcgcg ctccgtgatc gtgccgcgat gcgcggacgc
     4201 gagtgcgatc gcgcgatcga accaaccgag cgcctcgcgc acggcgaaga gcttcaacga
     4261 ccgctgcgcg acgagcacca tgtggtgcaa cgccttgccc cacacgtggc cgcgctcgta
     4321 atgctcggcg agccgcgcgt cgcgttcgtg cgcctcgcct tcgtcctgct gctccaaggc
     4381 ctcggcgacg gccttgtgca gcagccggcg ccccgcgccg ccgatctcga gataggctac
     4441 ttcgcgtacc ttgtcgtggc tgaagtcata ggttcccgcg gattcttcgc gcagcacgcg
     4501 ccaccgcacg agcgtctcca cggcatgcag cagctcgtcc tcggaggtct tcatgaccgc
     4561 gaacaggctc tcgaagtcga agcgccggcc gagcacggcc gcgacgtcga gcgccggccg
     4621 agcgtcgtcg ggaaggtgcg cgagccgcgc gcgtaccgat gcgcgcagcg cgtcgggcag
     4681 cggcagctcg gcgtccgcgt gggcggcgat ctcgccggcg cccatcgcct gcagcatcga
     4741 ggtcaggaaa aaaggattgc cgtcggtttc acggtgcagc cggaccgcca agcgctcgga
     4801 gaggtcggcc ccttggctcg cgagcaactc cttcgtttcg gcgagctcga gtctcgcgag
     4861 cgtcatgtgg tgcacgtgcg cctcgccgcg cacgctcgcg acgagctcgg cgagacccgc
     4921 gcgggtatcg agctcctcgt cgcgataggc gtagacggtc agcatcggca aagcagccgt
     4981 ccggcgcgcg aggtaatgca gaaactgcgc gctcgcgtcg tcggcccatt gcaaatcgtc
     5041 gacgataacg agcaggtgcc gcgtcgcagt gagcagctcg agaagctgca cgagcgcgtg
     5101 gaagatgcgc gcctgacggg cttcgggtag atcggccgaa agcaccggca ccgcggcgcg
     5161 cagcgcgacg ccgggcacga gcgccgcgat ctcggcgagc gagaccggcg gcagcttctg
     5221 cagcacggcg gtgggcatga gctcgagcgc ctgcgtgacg acgtcgatca cggcctgata
     5281 cggcatcgcc gcttcgatct cgtagcactt cgttgcgagc gtcgcggctc cgcgggcgcg
     5341 cgcctgacgc gcgagctcgc gcgtcagccg gctcttgccg attcccggct cgccttcgac
     5401 gagcgccacg cggccgacgc ccggcccgag ccgcgcgagc agccccgcga gcaggtcgat
     5461 ctctttgccg cgcccgacga acgggcccgc ttccgcttgc tcgacgcggt tcggcgtgag
     5521 cggcgtgttg tcgaccgcca agcgatcgac cgagcgcagc gcacgcgcag cctcgaggaa
     5581 cgcgccgcgc tcgtgcggcg gaattccgag ccggtcggcc agccgctcgg cgagccggcg
     5641 cgacgggcgg cgctcgtcgg cctcgatctt gcgaatggcg aagtgcgagc acgcgacgcc
     5701 ctgcgcgagc tgctcctggg tcaggtcgag cgccttacgg cgccgcttca gccaatagcc
     5761 gaacgatgtc gttcgatcat ccaagggata cgccttgccg ccgtcgtcgg cgccggggcc
     5821 ttcttctggg gcccctttgt gccgattctg gcggtttttt gtcccgctgt ctgtcccgct
     5881 cggcgacccc ggccgcgccg ctcgctccgc ttcaattcac gcgttcgaca aaacgcggcg
     5941 gcagctgccg cacgccattc gatcaagaca atgggaggag ctttcatgaa cgtatcgagg
     6001 aagagattcg tcggcccggc actgctctgg tgcgcggccg cggcactttc gac
//
LOCUS       DASSNN010000731         4824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_358353, whole genome shotgun sequence.
ACCESSION   DASSNN010000731 DASSNN010000000
VERSION     DASSNN010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358353"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1023
                     /locus_tag="VFL84_13740"
     CDS             49..1023
                     /locus_tag="VFL84_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET7609737.1"
                     /translation="MRPRIALVHDAAAAAGRPDSSDTLVEAQAIAAELAKLGYETLTL
                     PVGLDLGALERTLRELAPHAVFNLVESLVGRGRLLHLVPALLESLGVPFTGCSAHALA
                     ATSHKVAAKKLLRQADIATPAMLGEAGDDGPWIVKSVFEHASLGLDDSSVVRGASGAA
                     RILEARRAEFGGDWFAERFVAGRELNVALIAAPPGVRVLPVAELRFEGYPEDKPAIVG
                     YAAKWDSSSFEYRHTVRSFGVEPELAARAERLARACWDLFALDGYARVDFRVDASGLP
                     SVLEINANPCLSPDAGFAAALAQAGIGYRDAIGWLIADARRRVPARDA"
     gene            1020..1559
                     /locus_tag="VFL84_13745"
     CDS             1020..1559
                     /locus_tag="VFL84_13745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7609738.1"
                     /translation="MTKPRAHRYRSRPRPRDIAAVRRLVSSSGGVFQREERAIALELL
                     KERLARGAKSGYWFFFVERQAELLGYAAWGPVPLTRRSFDLYWIVVAGAVQRQGLGRA
                     LLKLVERGVAARGGGNLYIETSSKPEYSRTRRFYREAGYERVALLRHFYAPGDHKLMF
                     CKVIRARRQGVRGAGRVLL"
     gene            1746..1973
                     /locus_tag="VFL84_13750"
     CDS             1746..1973
                     /locus_tag="VFL84_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609739.1"
                     /translation="MRKAKAQVDEDGNEAHFFDDLESSDDLGDSDDDPDPRKPKKPAR
                     QRVELRNEQQSLRRQLSDWDDYEQDDFDTKL"
     gene            2102..3292
                     /locus_tag="VFL84_13755"
     CDS             2102..3292
                     /locus_tag="VFL84_13755"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HET7609740.1"
                     /translation="MLVDGESLDAAPAADALLEAAGGKPAEQSEIALSFGLVLHSIEL
                     RREPRREHETLPETLAANVRRANGLLAREGLRLLPTAMHPWLEPGDARLWPHGTPGAV
                     HAALDRLFSCKTHGWANQQSFAIELPFDGDEQFVRLHAAVRFLLPIVPALAASSPIVG
                     GERNGVADNRLAAYRGKCARLPAAIGAVVPEPVGSIGEYRERVLEPLYRELEPHDPEG
                     LLRQDWINARAAFVRLDRSSVELRVLDLQEAPAMDAAFAALIVEVLKLLCAEQWLDAA
                     GMNRRRTEELGRLLELAARQGEGLPVGDRPYLQALGFRGGATELKGLWEHLIDTVSAR
                     GSLSAANGRRLEHYLRHGTLATRIGKAVGLAPTRAKLMRVYEELCEALAGGTPFAPPP
                     PAAH"
     gene            3315..3968
                     /locus_tag="VFL84_13760"
     CDS             3315..3968
                     /locus_tag="VFL84_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HET7609741.1"
                     /translation="MPAAYAPLFAGAADVLATHRGYDLGAREVARAFGRRLGVTPFVG
                     TTTRLVVDLNRSPGNRSVFSAYTRPLSAVECEAALASHYWPYRNAVEDSVARAVGAGE
                     AVLHVSAHSFTPVLRGEVRNCDVGFLYDPREAGEVRFVESWYAALAAAAPRLTLRRNY
                     PYRGVSDSLVTHLRRRYAGRGYVGMELEVNQKHVGSASWRALVAVLAATLVTAVERG"
     gene            4027..>4824
                     /locus_tag="VFL84_13765"
     CDS             4027..>4824
                     /locus_tag="VFL84_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET7609742.1"
                     /translation="MSADPSLRNEQTIAVFVDFENLAFGVEEDKKGRFDIELVLKRLL
                     ERGRIVFKRAYCDWTRFKDFMHEFHRHGFEMVDIPSSKVSGKNSADIRMVVDAIDLCY
                     SKTHIDTFVLLTGDSDFSPVVSKLKENNKRVVGCGVRSSTSDLLANNCDEFIFYDDLI
                     RVQKRTQTRQKPKKESANKGEDKAEDKIEKRRQEAFDRLAATVASLESDYDPVWGSLV
                     KPTLKRVYPGFNESYYGYRNFTELLEDAAKEGVIKLELDEQRGNYKVR"
BASE COUNT          726 a         1689 c         1660 g          749 t
ORIGIN      
        1 atcgcgcgct gatcggcgag atcgtgcgct gcgcgtcggc gaggatcgat gcgtcctagg
       61 atcgcgctgg tccacgacgc cgcggccgcc gcgggccgcc ccgactcgag cgacacgctc
      121 gtcgaggcgc aggcgatcgc cgccgagctc gcgaagctcg gctacgaaac gctgacgctg
      181 ccggtcgggc tcgatctcgg cgcgttggaa cgcacgctgc gcgagctcgc gccgcacgcc
      241 gtcttcaatc tcgtcgagtc gctcgtgggg cgcggccggc tgttgcacct cgtgccggcg
      301 ctgctcgagt cgctcggcgt gccgttcacg ggctgctcgg cgcatgcgct cgcggcgacg
      361 tcgcacaagg tggccgcgaa gaagctgctg cggcaagccg atatcgcgac gccggccatg
      421 ctcggcgaag ccggcgacga cgggccctgg atcgtgaagt ccgtgttcga gcacgcgtcg
      481 ctcgggctcg acgactcgtc cgtcgtccgc ggcgcgagcg gcgcggcgcg cattctcgaa
      541 gcgcgccgcg cggaattcgg cggggattgg ttcgccgagc gcttcgtcgc cggccgcgag
      601 ctcaacgtcg cgctgatcgc ggcgccgccg ggcgtgcgcg tgctgccggt cgcggagctt
      661 cggttcgaag gctacccgga ggacaagccc gcgatcgtcg gctatgcggc gaagtgggac
      721 tcgagcagct tcgagtaccg gcacacggtc cgctcgttcg gcgtggagcc cgagctcgcc
      781 gcgcgcgccg agcggctcgc gcgcgcctgc tgggacctgt tcgcgctcga cggttatgcg
      841 cgcgtcgact ttcgcgtcga cgcctcaggt ctcccgtccg tgctcgagat caacgcgaac
      901 ccgtgcctct ctccggatgc cggcttcgcg gcggcgctcg cgcaggcggg cattggatac
      961 cgcgacgcga tcggctggct gatcgccgac gcgcgccgcc gcgtgcccgc ccgcgacgca
     1021 tgacgaagcc ccgcgcccac cgctatcgct cgcggccgcg gccccgtgac atcgcggccg
     1081 ttcgccggct cgtctcctcg agcggcggcg tattccagcg cgaggagcgg gcgatcgcgc
     1141 tcgagctgct caaggagcgc ctcgcgcgcg gagcgaagag cggctactgg ttcttcttcg
     1201 tggaacggca agccgagctc ttggggtatg cggcgtgggg gcccgtgccg ctcacgcggc
     1261 ggtccttcga cctgtactgg atcgtcgtcg caggcgcggt gcagcgacag ggcttgggcc
     1321 gcgcgctgct caagctcgtc gagcgcggcg tggcggcccg cggcggcggc aatctctaca
     1381 tcgagacctc gtcgaaaccc gagtacagcc ggacacggcg cttctataga gaggcgggct
     1441 acgaacgtgt cgcgctgctg cgccacttct atgcgccggg cgatcataag ctgatgtttt
     1501 gtaaggtcat tcgcgcccgc aggcagggcg ttcgaggcgc cggccgagtg ctgctctgac
     1561 ctaaagaaat cttataactg ccggatttcc ctagccattt cgcggcttga cacacttttg
     1621 caaccgccta tagttgcgca gccgaccgct cccgaggggt aggccgtgtc taacgggtgt
     1681 gccgcctgtt gggcacaacc ggaccgaaga tccggacagt actaggcaag gagggtcttt
     1741 accccatgcg taaggccaaa gcacaggtcg acgaagacgg caacgaggcc catttcttcg
     1801 acgacctcga gtcgagcgac gatctcggcg attccgacga cgatcccgat ccacgtaaac
     1861 ccaagaagcc cgcgcgtcag cgcgtcgagc tgcgcaacga gcagcagagc ctgcggcgcc
     1921 agctatcgga ctgggacgat tacgagcagg acgacttcga cacgaagctg tagcgcgccg
     1981 ccaagacggc ggccgtccgg cgcaagccac gattaccggg agcccttcca cgccgtcccc
     2041 tgatgcgccg cccgagcggc cgcttgcgct gctcgagggc tatggcgtcg cggccgagta
     2101 catgctcgtc gacggcgaga gcctcgacgc cgcgccggcc gcggatgcgc tcctcgaagc
     2161 ggcgggcggc aagccggccg agcagagcga gatcgccttg agctttggcc tcgtcctgca
     2221 ctcgatcgag ctgcggcgcg agccgcgccg cgagcacgag acgctgcccg agacgctcgc
     2281 cgcgaacgtg cgccgcgcga acggcctgct cgcgcgcgag gggctgcgct tgctgccgac
     2341 ggcgatgcac ccgtggctcg agccgggcga cgcgcggctc tggccgcacg gcacgccggg
     2401 cgccgtccat gccgcgctcg accggctgtt ctcgtgcaag acccacggct gggcgaacca
     2461 gcagagcttc gcgatcgagc tgccttttga cggcgacgag caattcgtgc gcctgcacgc
     2521 cgcggtgcgg ttcctgctgc cgatcgtgcc cgcgctcgcc gcgagctcgc cgatcgtcgg
     2581 cggtgagcgc aacggcgtcg cggacaaccg cctcgccgcg tatcgcggca agtgcgcgcg
     2641 cttgcccgcc gcgatcggcg ccgtcgttcc ggagcccgtc ggctcgatcg gcgagtatcg
     2701 cgagcgcgtg ctcgagccgc tctaccgcga gctcgagccg cacgaccccg aaggcctctt
     2761 gcgccaggac tggatcaacg cgcgcgccgc gttcgtgcgc ctcgatcgct cgagcgtcga
     2821 gcttcgcgtc ctcgacctgc aggaagcgcc cgccatggac gccgcgtttg cggcgctgat
     2881 cgtcgaggtg ctgaagctgc tctgcgccga gcagtggctc gatgccgcgg gcatgaaccg
     2941 gcggcgcacc gaggagctcg gccggttgct cgagctcgcc gcgcggcaag gcgaggggct
     3001 tccggtcggc gaccggccgt atctgcaagc gctcggtttc cgcggcggcg ccaccgagct
     3061 caaggggctc tgggagcatt tgatcgacac ggtctcggcg cgcggctcgc tgtccgcggc
     3121 gaacggccgg cggctcgagc actatctgcg ccatggcacg ctcgcgacgc ggatcggcaa
     3181 agccgtcggg ttggcgccca cgcgggccaa gctcatgagg gtctatgaag agctctgcga
     3241 agcgctggcc ggcggcacgc cgttcgcgcc gccgcctccg gccgctcatt agctgcgagc
     3301 acggcggcaa ccgcgtgccc gcggcctacg cgccgctgtt cgcgggcgcc gccgacgtgc
     3361 tcgccacgca tcgcggctac gacttgggcg cgcgcgaggt cgctcgcgcc ttcggccgcc
     3421 ggctcggcgt cacgccgttc gttggcacga cgacgcgcct cgtcgtcgac ttgaaccgtt
     3481 cgccgggcaa ccgcagcgtg ttctcggcgt atacgcgccc gctgtcggcg gtcgaatgcg
     3541 aggcggcatt ggcctctcac tactggccgt accgcaatgc cgtcgaggat tccgtcgcgc
     3601 gcgccgtcgg cgccggcgag gccgtgctgc acgtgtcggc acacagcttc acgccggtgc
     3661 tgcgcggcga ggtccgcaac tgcgacgtcg gcttcctcta cgatccccgc gaagcgggcg
     3721 aggtccgctt cgtcgagtcg tggtacgccg cgctcgctgc ggccgccccg cggctcacgc
     3781 tgcggcgcaa ctatccgtat cgcggcgtct ccgactcgct cgtgacgcac ttgcgccgcc
     3841 gctacgccgg ccgcggctac gtcggcatgg agctcgaggt gaaccagaag cacgtcggca
     3901 gcgcaagctg gcgcgcgctc gtcgcagtgc tcgccgccac gctcgtgacg gccgtcgagc
     3961 gcggctagct tgcccggccg acgcgttcgc cctcactatg cggggcgcaa cccaaaggaa
     4021 ccgcccatgt ccgccgaccc ttcgctgcgc aacgagcaaa cgatcgccgt gttcgtcgat
     4081 ttcgagaacc ttgcgttcgg cgtcgaggag gacaagaaag gccggttcga catcgagctc
     4141 gtgctgaagc gcctgctcga gcgcggccgc atcgtcttca agcgtgccta ctgcgactgg
     4201 acgcggttca aggatttcat gcacgagttc caccgccacg gcttcgagat ggtcgacatc
     4261 ccgagctcga aggtgtccgg taagaacagc gccgacatcc gcatggtcgt cgacgcgatc
     4321 gacctctgct actcgaagac gcacatcgac acgttcgtgc tcttgaccgg cgacagcgac
     4381 ttttcgcccg tcgtctcgaa gctcaaggag aacaacaagc gcgtcgtcgg ctgcggcgtc
     4441 cgcagctcga cctcggacct gcttgccaac aattgcgacg agttcatctt ctacgacgac
     4501 ctcattcgcg tgcagaagcg cactcagacg cggcagaaac cgaagaagga gagcgcgaac
     4561 aagggcgagg acaaggccga ggacaagatc gagaaacgca ggcaggaagc gttcgaccgg
     4621 ctcgccgcga cggtcgcctc gctcgagtcg gattacgacc ccgtctgggg ctcgctcgtg
     4681 aagccgacgc tgaagcgcgt atacccgggg ttcaacgaga gctattacgg ctaccgcaac
     4741 ttcacggagc tgctcgagga cgccgccaag gaaggcgtca tcaagctcga gctcgacgag
     4801 cagcgcggca actacaaggt ccgc
//
LOCUS       DASSNN010000732         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1872240, whole genome shotgun sequence.
ACCESSION   DASSNN010000732 DASSNN010000000
VERSION     DASSNN010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872240"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFL84_13770"
     CDS             complement(<1..498)
                     /locus_tag="VFL84_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HET7609743.1"
                     /translation="MMTYDTLFSIRRLWIILAASMVVMFGTLLYFGGQIYQAAPPIPA
                     AVTTAGGETLFTRADIERGQNVWQSIGGMQQGSIWGHGSYVAPDWSADWLHREAVALL
                     DLLAREDGASSYGALAAPEQARLQAALAQTMRTNTYDAATGVVTVSDERARAIASVAL
                     HYSGLF"
     gene            complement(547..1179)
                     /locus_tag="VFL84_13775"
     CDS             complement(547..1179)
                     /locus_tag="VFL84_13775"
                     /inference="COORDINATES: protein motif:HMM:NF008228.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family NAAT transporter"
                     /protein_id="HET7609744.1"
                     /translation="MNFAAELFYYVLSTIGALLPIVNPFAAAPMVVSMTAPLAEDERR
                     DQIRRACIYMFCILAAFLVAGGLIMQFLGISIPGLRVAGGLVIAAVGFRMLFLPQPST
                     AYAEQGPKHDISFTPLAIPSLAGPGSIAVVIGMSTTAQADRYVILRHALIIVGIAITA
                     LVCYFVLRGATRLARFLGPSGMSGLTGIMGFLLVCIGVQFVINGVTHLEG"
     gene            1391..2239
                     /locus_tag="VFL84_13780"
     CDS             1391..2239
                     /locus_tag="VFL84_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018295028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HET7609745.1"
                     /translation="MARLEQSPAAAGLVRKAARQATRAELEYFHTPAYVDRVIELSAR
                     GSGLLDAGDTPAFPGVYEAAASVVGGTLEALAAIVAGPARRAFIPIGGLHHAARDGAA
                     GFCVFNDIGVAIEAARRHHGIRRVAYVDIDAHHGDGVFYAFEADPEVKFADLHEDGRY
                     LYPGTGSRSETGQGPAAGTKLNIPLPPGADDSVFLAAWEEVERYVEKAEPELILLQCG
                     ADSIAGDPITHLQLTPEAHAHAARRLAALADRFAEGRVLGMGGGGYNRANLANAWTRV
                     VEELAA"
     gene            2239..>2735
                     /gene="glyA"
                     /locus_tag="VFL84_13785"
     CDS             2239..>2735
                     /gene="glyA"
                     /locus_tag="VFL84_13785"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011589454.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET7609746.1"
                     /translation="MFAKQSLDEFDPELGSVLAAEGIRQETYLELIASENYVSPRVLE
                     AQGSLLTNKYADGYPGHRHYAGCEMADVAERLAIERACELFGAGWANVQPYSGSQANL
                     AAYLAVLSPGDTLLGMRPAHGGHRTHGAADNQSGSLYNVVPYDVAATGDLDYDQIAAL
                     ARKHRP"
BASE COUNT          471 a          953 c          902 g          409 t
ORIGIN      
        1 aaaaaggccc gaatagtgca gcgcgaccga tgcaatcgcg cgcgcccgct cgtcgctgac
       61 cgtcacgacg ccggtcgccg cgtcgtacgt gttcgtgcgc atcgtctgcg ccaaggccgc
      121 ttgcaatcgc gcctgctccg gcgccgcgag cgcgccgtag ctcgatgcgc cgtcctcgcg
      181 cgcgagcagg tcgagcagcg cgacggcctc gcggtgcagc cagtcggccg accaatccgg
      241 cgcgacgtag ctgccatggc cccagatcga gccctgctgc atgccgccga tcgattgcca
      301 gacgttctgg ccgcgctcga tgtcggcgcg cgtgaacagc gtctctccgc ccgcggtcgt
      361 cacggcggcc ggaatcggcg gtgccgcttg gtagatctgc ccgccgaagt aaagcagcgt
      421 gccgaacatc acgaccatgc tcgccgccag gatgatccag aggcgccgga tcgagaacag
      481 cgtgtcgtaa gtcatcatga aaccccccga ttagcgctcg ccgctcgccc gccggccact
      541 tcgtgcctag ccttccagat gtgttacgcc gttgatgacg aattgaacgc cgatgcagac
      601 gagcaggaat cccatgatgc cggtcaagcc gctcatcccg ctcggcccga gaaaccgcgc
      661 gagcctcgtg gcgccgcgca gcacgaagta gcacacgagc gccgtgatcg cgattccgac
      721 gatgatcagc gcgtgccgca ggatcacgta gcggtcggct tgtgccgtcg tcgacatccc
      781 gatcacgacg gcgatcgagc cggggccggc caagctcggt atcgcgagcg gcgtgaacga
      841 gatgtcgtgc ttcggcccct gctcggcgta ggccgtgctc ggttgaggca ggaacagcat
      901 gcggaaaccc acggcggcga tgacgagccc gccggcgaca cgcaggcccg gaatcgagat
      961 gccgagaaac tgcatgatca ggccgcccgc cacgaggaac gcggccaaga tgcagaacat
     1021 gtagatgcag gcccggcgga tttgatcgcg gcgttcgtcc tcggcgagcg gcgccgtcat
     1081 gctgacgacc atcggcgccg ccgcgaacgg gttgacgatc ggaagcagcg cgccgatcgt
     1141 gctcaacacg taatagaaca gctctgcggc gaaattcatc tggcccggta tcatgcccgc
     1201 agcaaaaatc ggccgccagc acggcgccgc gtgccgcctc gcgcatgcgg ttcgtgcgat
     1261 catttagccg tcgcaacgca gcggtaaccg caagatggcg caagcgctcg tccttgtctc
     1321 gggccccgag attgcccggt atggatttcc gcagggacat ccattcggca ccgatcgcca
     1381 tgccgcgttc atggcgcgac tcgagcaatc gccggccgcc gcaggcctcg tccgcaaggc
     1441 ggcgcggcag gcgacgcgcg cagagctcga gtacttccac acgccggcat acgtcgatcg
     1501 cgtcatcgag ctgtcggcgc gcggcagcgg cttgctcgat gccggcgata cgccggcgtt
     1561 tccgggagtc tacgaagccg ccgcgagcgt cgtcggcggc acgctcgagg cgcttgccgc
     1621 gatcgtcgcg gggccggcgc ggcgcgcgtt cattccgatc ggcggtttgc atcacgcggc
     1681 gcgcgacggc gccgcgggct tctgcgtctt caacgacatc ggcgtcgcga tcgaagccgc
     1741 gcgccgccac cacggtattc gccgcgtcgc gtacgtcgac atcgacgcgc accacggcga
     1801 cggcgtgttc tacgcgttcg aggcggaccc cgaggtcaag ttcgccgacc tgcacgagga
     1861 cggccgctat ctctatcccg gcaccggcag ccgcagcgag accgggcagg gccccgcggc
     1921 cggcacgaag ctcaacatcc cgttgccgcc gggcgccgac gactcggtct ttctcgcggc
     1981 atgggaagaa gtcgagcgct acgtcgagaa agcggagccg gagctgatcc tgctgcaatg
     2041 cggcgcggac tcgatcgccg gcgatccgat cacgcattta cagctcacgc ccgaggctca
     2101 cgcgcacgcg gcgcgccggc tcgccgcgct cgcggaccgg ttcgccgaag gacgcgtgct
     2161 cggcatgggc gggggcggat acaaccgcgc gaacctcgcg aacgcgtgga cgcgcgtcgt
     2221 cgaagaattg gcggcctgat gttcgccaag caatcgctcg acgaattcga tccggagctg
     2281 ggaagcgtgc tcgctgccga gggcatccgc caggaaacgt acctcgagtt gattgcgtcc
     2341 gaaaactacg taagcccgcg cgtgctcgaa gcgcagggct cgctcctcac caacaagtat
     2401 gccgacggct accctggcca ccgtcactac gccggctgcg agatggcgga cgtcgccgag
     2461 cgactggcga tcgagcgcgc ttgcgaattg tttggagccg gctgggccaa tgtgcagccg
     2521 tattccggct cgcaagcaaa cctcgccgcg tatctcgccg tgctctcgcc cggcgacacg
     2581 ctgctcggca tgcgcccagc gcacggcggc catcgaacgc acggcgcggc cgacaaccag
     2641 tcgggctcgc tgtacaacgt cgtgccgtat gacgtcgctg caaccggcga tctcgactac
     2701 gaccagatcg ccgcgctcgc gcgcaaacat cggcc
//
LOCUS       DASSNN010000733         3777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1508579, whole genome shotgun sequence.
ACCESSION   DASSNN010000733 DASSNN010000000
VERSION     DASSNN010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508579"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFL84_13790"
     CDS             complement(<1..322)
                     /locus_tag="VFL84_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7609747.1"
                     /translation="MTTSQPTVYVVDDDPSVLRAIGRLLRMEFAVEAFDSPATFLEAM
                     RPDAPGCILLDLALPGVSGLELQERLVASGCLQPVVFLSGQGSVRTSVQAMRAGALDF
                     IEKPW"
     gene            complement(319..2553)
                     /locus_tag="VFL84_13795"
     CDS             complement(319..2553)
                     /locus_tag="VFL84_13795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7609748.1"
                     /translation="MNRKQKAEWCAHFFACVAALLPAAAQAKDVLLLHPLEQRAPEFA
                     AIEAGLRREFANASVEATHVYSELVDLDFRGPIDRRIFADYLRAKYADVGIDAVVALS
                     DDAVQFVSAYRDVIGNVPLVFTTRASSATSNIPNSSSVVAPLFPSRTVELALRLQPSA
                     KHLFVVSDAPTADDVGLPPLRTFMPDYSGKLDVEYLLGLAPDALLDRVSHLPPSSLVL
                     YAPIDRTPVPDGLAPHEMAEQLAARANAPVYGVHDDDVGRGFVGGYMPRSAQIGAELA
                     RQTLRALRGEASRAADIGASYIVDSRALSRFKLDEARLPAGTELKFAPPSIWKLYQGW
                     IITLLVLVAVQFALIVTLLIQSISRRRDRLALAETAQRFQLARVAGKVGIWQWDRGTE
                     LLIVEPELRELLGYAASEGVEATRDWTKLIYEEDLPKFRRAARDHAQGVTPSFELQCR
                     ILDKNGHVRWFLCRGQALIGRVRLIGTATDITDRKRDEDERARTLLQLQEQRNELAHL
                     GRAAMAGALSGAVAHELNQPLSAMMSNARAGLQFIAGGSADRQEIKDILADIENDGRR
                     AGEVIRHLRSLLRRGEAQFETVQLDVIVDQVLRLINSDLISNNIKVVHEPMAGVPAIS
                     ADPVQLQQVMLNLLSNACDALKGVNPRDRRITIGVGMRGPGRLRISVSDNGCGFADGN
                     PEHLFKPFVTTKRSGLGLGLSISRSIIDAHGGRIWAENNRDAGATFHVDLALAAQGAE
                     RVVA"
     gene            2908..>3777
                     /locus_tag="VFL84_13800"
     CDS             2908..>3777
                     /locus_tag="VFL84_13800"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HET7609749.1"
                     /translation="MRVLSAIALAALVCAACEREAPVSPAQPAVAAADRAERESARLR
                     EWLDARYEEQLDFSPLTKTRLGRKEDYDRIDDLSEAGAEARLAWLRASVAELEREFDR
                     DALTAEAQTSYDLWRYGLERFEAAWPYRRRGYIFTQMQGPHTGLPQALINFHRVDEEA
                     DMLAYIARLGEVGRALRQALDAAQVAAGEGVHAPRFAYDAVIEQSRAVIAGAPFGGAG
                     QSPLYADANAKLAALVAAAKIDTARADELRAAAAAALTERVKPAYDDIIAWASADRAN
                     SGEIATGVWKLPDG"
BASE COUNT          624 a         1278 c         1285 g          590 t
ORIGIN      
        1 cccaaggctt ttcgatgaag tcgagcgcgc ccgcgcgcat cgcttggacg ctcgtgcgta
       61 cactgccctg gccgctcaag aagacgacgg gctgcaggca gcccgatgcg acgagccgct
      121 cctggagctc gaggccggac acgcccggaa gcgcgaggtc gagcaggatg cagcccggcg
      181 cgtcggggcg catcgcctct aggaaggtcg ccggagaatc gaacgcctcg acggcgaact
      241 ccatgcgcaa cagacgcccg atcgcgcgca gcacgctcgg atcgtcgtcc acgacgtaga
      301 cggtcggttg actcgtcgtc acgccaccac ccgctctgct ccctgcgccg cgagggcgag
      361 gtcgacatgg aacgtggccc ccgcgtcgcg attgttctcg gcccaaatcc taccgccgtg
      421 ggcatcgatg atcgatcgcg agatcgagag gcccaggccc aagccgctcc gcttcgtcgt
      481 gacgaacggc ttgaacagat gctcgggatt gccgtccgcg aatccgcagc cgttgtcgct
      541 gaccgagatg cggaggcgcc cggggccgcg catgccgacg ccaatcgtga tgcgccggtc
      601 gcgcgggttc acgcccttca gggcgtcgca cgcgttgctc aacagattca gcatgacctg
      661 ctgcagctga accgggtcgg cggagatcgc cggcacaccc gccatcggct cgtgcacgac
      721 cttgatgttg ttgctgatca aatcgctgtt gatgagccgc agcacctggt cgacgatcac
      781 gtcgagctgc acggtctcga actgcgcttc gccgcgccgc agcaagctgc gcaggtggcg
      841 aatcacctcg cccgcgcggc ggccgtcgtt ttcgatgtcg gcgaggatgt ccttgatctc
      901 ttgccggtcc gccgagcctc cggcgatgaa ctgcagcccg gcccgagcgt tgctcatcat
      961 cgcgctcaac ggctgattga gctcgtgcgc gacggcgccg gacagcgcgc ccgccatcgc
     1021 ggcacggccg agatgcgcga gctcgttgcg ctgctcctgc agctgcagca gcgtccgtgc
     1081 tctttcgtcc tcgtcgcgct ttcgatcggt gatgtcggtc gccgtgccga tgaggcggac
     1141 gcgcccgatc agcgcctggc cgcgacacaa gaaccagcga acgtggccgt tcttgtcgag
     1201 aatgcgacat tgaagctcga agctcggcgt gacgccctgc gcgtggtcgc gcgccgcgcg
     1261 gcgaaacttc ggcaggtctt cctcgtagat gagcttggtc cagtcgcgcg tcgcttcgac
     1321 accttcgcta gcggcgtatc cgagcagctc gcgcagctcg ggctcgacga tcagcagctc
     1381 ggtccccctg tcccactgcc agatgccgac cttgccggcg acgcgcgcga gctgaaaacg
     1441 ctgcgccgtc tcggcgagcg cgagccggtc tctgcggcgg ctgatcgatt ggatcaacaa
     1501 ggtcacgatc aacgcgaatt gcacggcgac gagcacgagc agcgtgatga tccagccttg
     1561 gtagagcttc cagatcgacg gcggtgcaaa cttgagctcg gtgccggccg gcaagcgcgc
     1621 ctcgtcgagc ttgaagcggc tcaacgcgcg tgagtcgacg atgtacgagg cgccgatgtc
     1681 cgcggcgcgc gaggcttcgc cgcgaagcgc gcgcagcgtc tggcgcgcca gctcggcgcc
     1741 gatttgcgcg gagcgaggca tgtagccgcc cacgaagccg cggccgacgt cgtcgtcgtg
     1801 gacgccgtaa acgggagcgt tggcgcgcgc cgcgagctgc tccgccattt cgtgcggggc
     1861 cagtccgtcc ggcaccgggg ttcggtcgat cggcgcgtag agcacgagcg agctcggcgg
     1921 caagtgcgag acgcggtcga gcagcgcgtc gggcgcgagg ccgagcaggt attcgacgtc
     1981 gagcttgccc gagtaatcgg gcatgaaagt gcgaagcggc ggcagtccca cgtcgtcggc
     2041 cgtcggcgca tcgctgacca cgaacaaatg cttcgcgctc ggctgcaagc gcagcgcgag
     2101 ctccacggtg cgcgacggaa agagcggagc aacgaccgac gatgagttcg ggatgttgct
     2161 cgttgccgag ctcgcccgcg tcgtgaacac caacggcaca ttgccgatga cgtcgcgata
     2221 tgcggagacg aattgcacgg cgtcgtcgga caacgcgacg acggcgtcga tgccgacgtc
     2281 ggcgtatttg gctcgcaagt agtcggcgaa gatgcgccga tcgatgggcc cgcgaaaatc
     2341 gaggtcgacg agctcgctat agacatgcgt cgcttcgacg gacgcgttcg cgaattctcg
     2401 gcgaagcccg gcctcgatcg cggcaaactc aggcgcacgc tgctcgagag gatgaagcag
     2461 cagcacgtct ttcgcttgcg ccgccgccgg caagagcgcg gctacgcacg caaaaaaatg
     2521 cgcgcaccat tccgccttct gctttcgatt cacttgacgg acccgctcgc ggcgcattct
     2581 gcgacgctcg agaggcatgc ggtgtgtgcg gcgccgcgct gcctagtgtt gtagtgttgc
     2641 tacgctaaca tagttcccgc gaatgccacc actatcccgc atagcgacgg cagtcaccga
     2701 ctgcgaactg cgcggtcgtc gcgtaccggc aacagaattc ccagttgccg gggctgtcgc
     2761 agggtggttt gctcagtacc tggctcgtta gcaaggctca ataccaaccg gggccggcgc
     2821 cgtccccggg cggagccgcg caccttatgg gtccggcacg gcgcggcctt gcagtaagct
     2881 ggcaaccccg tccgtggagg ctgtcgcatg cgcgtattgt ccgccatcgc cttggccgcc
     2941 ctcgtttgcg ctgcctgtga gcgcgaagca ccggtctcgc cggcgcagcc ggcagtcgcg
     3001 gcggccgacc gagccgaacg cgaaagcgcg cggctgcgcg aatggctcga cgcgcgctac
     3061 gaggagcagc tcgacttcag ccccctgacg aagacgcgcc tcggccgcaa ggaagactac
     3121 gatcgcatcg acgacctctc cgaggccggc gccgaagcgc ggctcgcctg gctgcgcgcg
     3181 agcgtcgccg agctcgagcg cgagttcgac cgcgacgcgc tgacggccga agcgcagacc
     3241 tcctacgatc tctggcgcta cgggctcgag cgcttcgagg cggcatggcc gtatcggcgg
     3301 cgcggctaca tcttcactca gatgcagggt ccgcataccg ggctgccgca agcgctgatc
     3361 aacttccacc gcgtcgacga agaagccgac atgctcgcgt acatcgcgcg cttaggcgaa
     3421 gtcgggcgcg cgctgcgcca agctctggac gccgcgcagg tcgcggccgg cgaaggcgtg
     3481 cacgcgccgc gcttcgcgta cgacgccgtc atcgagcaat cgcgtgcggt catcgcggga
     3541 gcgccgttcg gcggcgctgg ccagtcgccg ctctacgcgg acgcgaacgc caagctcgcc
     3601 gcgctcgtcg ccgcggcaaa gatcgacacg gcgcgcgcgg atgagctccg ggcggcagcc
     3661 gcggccgcgc tgaccgagcg cgtcaagccc gcgtacgacg acatcatcgc gtgggcatcg
     3721 gccgatcgcg cgaacagcgg cgagatcgcg acgggcgttt ggaagctgcc cgacggg
//
LOCUS       DASSNN010000734         6266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1873031, whole genome shotgun sequence.
ACCESSION   DASSNN010000734 DASSNN010000000
VERSION     DASSNN010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873031"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1107
                     /locus_tag="VFL84_13805"
     CDS             217..1107
                     /locus_tag="VFL84_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609750.1"
                     /translation="MTDSTPTIQGLRDRLTGLLADWGAELAVVLKELEDKRARVAELE
                     SAAASNAELEQLKQQLEGQTQLIETLRGDAEEAQKLRAEVRNRDLEFERVSSELESKK
                     ELVRALRRDTDGVDRVRTESRREVEELKSKLQKAEAQAKKAAAELAAIRETSDSKKSE
                     ELTELEALRADLEARKSLIKSLRSDQERIAPLQATLQEKQEIIAQLEAAMNRHSNTIA
                     ELRRSVETWRRKYLALKGDSPTATTSVTLPALSDTDVRIIEQVDKKGGGVNQQATIAI
                     DMRRSLLEARRSGPKGHGEK"
     gene            1460..3457
                     /locus_tag="VFL84_13810"
     CDS             1460..3457
                     /locus_tag="VFL84_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HET7609751.1"
                     /translation="MTTTLSENPVHRDLMGLKTSHHRGSFIDDLTAFATQHQAQHWRG
                     TFAEFLHDILPKDPAKLTRSSHQYVYDMLCAYRDAPRHVRGTGEPEPTATALFAGELF
                     GIDSALERVIDYFKAAAAGSDVGRRLLLLLGPPSGGKSSLVILLKRGLEEYSHTDDGA
                     LYALQGSPLHESPLNLIPASLRPKFRATYGVDIQGELSPYARARLDDEFQGDFMRFPV
                     ERIFLSEAARVGVGTYAPHDPTTADIADLVGSVDLSKVADIGDEGDPRAWSWSGAVYA
                     ASRGVLEMIEILKVKREFLYLLLTLTQEKNVKVSRFPLIHLDETILAHTNLAEFNKFL
                     QEKENEALLDRMVIVKVPYTLSFRDEARIYRKLVSSAPAFRKVHFDPHVVDLAAVFAI
                     LTRLQKPTREGLDLTKKLKLYAQEDVEGFTAADVPRIRQESTDEGLTGVSPRFVINAI
                     SNAITRNNMASLTSMDMLLALKDAIETDARMDAGRKKQWIEFLVLARKDFYNRWVKED
                     VHRALFASFQEEAQQLLEKYLDEIEASLDKREVEDPITGESRPPDERFLRSVEEKIKI
                     SESGKLSFRQEVVRKAMVAFKAGEKFTLDNHSRLHEAIEQYLFEERRDVLRLVTSKAR
                     PDDEAEHKISAVQERLIKEYGYDEHSAKEALNYVTTLLSQE"
     gene            3491..4651
                     /locus_tag="VFL84_13815"
     CDS             3491..4651
                     /locus_tag="VFL84_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HET7609752.1"
                     /translation="MAASSGFVDPSGSGRQWYDLFSRGARDWLRHNDKVRAAVRDKLP
                     ELIAGSELLPSADRTVQVPVRFLEHARLRLRDAEQSEGVGQGAGKPGDQLVQPSDGTM
                     SKGAGGNEEGGYQFILELKIDDIVDWLWEELKLPNLKVKSGAVQYDDYTREGWSRRGV
                     RSRLDRRRSLKEAIKRRAVQDEGAAFTNDDLRYRQLAMRRRPATEAVVYFAMDVSSSM
                     TENDRKLAKTFFFWVVQGLRRQYTHLETVFVAHTVEAWEFAEQEFFQVSGSGGTVAST
                     AFRKVLEIATHRYDPGRFNTYVFYASDGANFHNDREAAQAALNELGEIANYVGYVETA
                     SVAQQPLQSETALLFEQLEAATHAAGRHALSSPESVWDAIRAFFSRRAEAEV"
     gene            4653..6020
                     /locus_tag="VFL84_13820"
     CDS             4653..6020
                     /locus_tag="VFL84_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HET7609753.1"
                     /translation="MNEALQTYAGRLEALAKSHGLDYYPVQFEEVPSSFMMEIAVYGL
                     PVRMPHWSFGVRYIYQLVQHRMGHSRLFEVVFPGNPGRAYLAGNNSLQENTLVAAHVL
                     GHADFAKNNSLFKRSQEQVGYRIVEQAAAHARQIGAAVEQYGQNRVEQVLDAALSLEA
                     HIDPIQLLRRDRYPQYVEAPRPVQESGFQRRFESLPGSPEVVDAPLERARAPIPAAPE
                     RDLLWLIAEYAPELEEWERDIFRAVREESFYFYPVFACQIMNEGWASYWHARLLREAD
                     FLPQNEYLDAIKTHSDVVRPHAAEQQASLATNPYHLGFSIWESIVKEHGIERAFQIRA
                     EDDDFSFVRNHLTEELAEELKLFRYERSADGGVKVLDHDIHALRETVLAPKYNFGAPA
                     VLAKHIAHDGALTLAHEHDTDGRGLDLDRAERVLDYLHKVWRRPIRLETIDSSGQPRT
                     ISRAA"
BASE COUNT         1219 a         2057 c         2010 g          980 t
ORIGIN      
        1 atcgacgagt ccgagccgga catgcacggc gtcaacggca ccgtggcggc accgctcagc
       61 cgcctgccct acgaggacct gtgcccgggg cgtgacgcgc tgctcgcgtt gcagaagcgg
      121 ttccgctgaa tagccgcgca tgtgacgtaa ttcaccgcaa gaattcgcga agtccggcag
      181 cgtagcgggc gctacaagaa gtagcgtggg gacgctatga ccgactcgac tcctacgatc
      241 cagggcttgc gcgatcgctt gaccggcctt ctggccgatt ggggagccga gctcgcagtc
      301 gtcttgaagg agctcgagga caagcgcgcg cgcgtggccg agctcgaaag cgccgccgcc
      361 agcaatgccg agctcgagca gctcaagcag cagctcgagg gccaaaccca actgatcgag
      421 acccttcgcg gcgacgccga ggaagcgcag aagctgcgcg ccgaggtgcg caaccgagac
      481 ctcgagttcg agcgcgtgag ctcggagctc gagagcaaga aggagctcgt gcgagcgctg
      541 cgccgggata ccgatggcgt cgaccgcgtg cggaccgagt cgcgccgcga ggtcgaagag
      601 ctcaagagca agctgcagaa agctgaagcc caggcgaaaa aggccgcggc ggagctcgcg
      661 gccatacgcg agacgagcga tagcaagaag agcgaggagc tcacggagct cgaggcgctg
      721 cgcgccgact tagaggcgcg caagtcgctg atcaagagcc tgcgctccga tcaggagcgc
      781 atcgcgccgc tgcaggcgac gctgcaagag aagcaagaga tcatcgcgca gctcgaggcc
      841 gccatgaacc gtcactcgaa cacgatcgcc gagctgcgcc gcagcgtcga gacctggcga
      901 cgcaaatacc tggccttgaa gggcgacagc ccgaccgcga cgacgtcggt gacgttgccg
      961 gcgctctcgg acaccgacgt gcgcatcatc gagcaagtcg acaagaaggg cggcggcgtc
     1021 aaccagcagg ccacgatcgc gatcgacatg cgccgctcgc tgctcgaggc gcgccgctcg
     1081 ggcccgaaag gccacggcga gaagtagccg gcccggtaga tccgcgccgt tttccggccg
     1141 cgcgctcgca agcgccggca gcaatttccc aaatcgttaa aaaatagagc ggagccgcct
     1201 ccgtcggcga cgcttgcgcg tcgtcgcgat cttgtcgaac ttatgttcaa ccgatcctat
     1261 aattgtgact gtactcacag acacgccgct tggaaagttc gagcctgcat ctaacgtcga
     1321 tttaacggaa cgccgattac gttacttcgt ctaaatcgtt gtatcggtag ttggaggaga
     1381 gcctgacgat caaacaacaa caataaaaaa aacgaaggca atgcccatgg catcgcaaac
     1441 atcaaaatag ggagtgttga tgacgacgac gctatccgag aacccagtcc atcgagatct
     1501 gatgggcctc aaaacgtcac accacagagg ctcgttcatc gatgacttga ccgcgttcgc
     1561 cacgcaacat caagcacagc actggcgtgg caccttcgcc gaattccttc acgacattct
     1621 tcccaaagat cccgccaagc tgacgcgctc gtcgcatcag tacgtctacg acatgttgtg
     1681 cgcgtatcgc gacgcgccga gacacgtgcg tggaacggga gaaccagagc cgacggcgac
     1741 cgcgttgttc gcgggcgagc tgttcggcat cgacagcgct ctcgagcgcg tcatcgacta
     1801 cttcaaagcc gcggccgccg gctccgacgt cggccggcga ttgctgctat tgctaggccc
     1861 gccgtcgggc ggcaagtctt cgctcgtgat tctcttgaag cgcggcctcg aggagtacag
     1921 ccacaccgac gacggcgcgc tgtacgcgct gcaaggctcg ccgctgcacg agtcgccgct
     1981 gaacctgatc cccgcgagcc tgcggcctaa gttccgcgcg acctacggcg tcgacatcca
     2041 aggcgagctc tcgccgtacg cgcgcgcgcg gctcgacgac gagttccagg gcgacttcat
     2101 gcggttcccg gtggagcgca ttttcctgtc cgaagccgca cgcgtcggcg tcggcacgta
     2161 cgctccgcac gacccgacga cggccgacat cgcggacttg gtcggctccg tggacctctc
     2221 gaaggtggcc gacatcggcg acgaaggcga cccgcgcgcg tggtcctggt cgggcgccgt
     2281 ctacgccgcg agccgcggcg tgctcgagat gatcgagatc ctgaaggtca agcgcgagtt
     2341 cctgtatctg ctcttgacgc tgacgcagga gaagaacgtc aaggtgtcgc gcttcccgct
     2401 gatccatctc gacgagacaa tcctcgcgca tacgaacctg gccgagttca acaagttctt
     2461 gcaggagaag gagaacgagg cgctgctcga tcgcatggtg atcgtcaagg tgccgtacac
     2521 gctctcgttc cgcgacgagg cgcgcatcta tcgcaagctc gtctcgagcg cgccggcgtt
     2581 ccgcaaagtg cacttcgatc cgcacgtcgt cgatctcgcc gccgtgttcg cgatcctgac
     2641 gcgcttgcag aagccgactc gcgaaggcct tgacctcacg aagaagctca agctctacgc
     2701 ccaggaggac gtcgaaggct tcacggcggc cgacgtgccg cgcatccgcc aggaatccac
     2761 cgacgaaggt ttgactggcg tgagcccgcg cttcgtcatc aacgcgatca gcaacgcgat
     2821 cacgcgcaac aacatggcga gcctgacgag catggacatg ctgctcgcac tgaaggacgc
     2881 catcgagacc gacgcgcgca tggacgcggg ccgcaagaag cagtggatcg agttcctcgt
     2941 gctcgcgcgc aaggacttct acaaccgctg ggtcaaagag gacgtgcacc gcgcgctgtt
     3001 cgcgtcgttc caagaggagg cgcagcagct tctcgagaaa tatttggacg agatcgaggc
     3061 ctcgctcgac aagcgcgagg tcgaggatcc gatcacgggc gagtcgcgcc cgcccgacga
     3121 gcggttcctg cgctcggtcg aggagaagat caagatctcc gagtccggca agctctcgtt
     3181 ccggcaggaa gtcgtgcgca aggcgatggt cgcgttcaag gccggcgaga aattcacgct
     3241 cgacaatcac tcgcgcttgc acgaggccat cgagcagtac ctgttcgagg agcgccgcga
     3301 cgtgctgcgg ctcgtgacct cgaaggcgcg gcccgacgac gaggccgagc acaagatctc
     3361 ggccgtccag gagcgcctga tcaaagagta cggctacgac gagcacagcg cgaaagaagc
     3421 gctcaattac gtcaccacat tgttgtcgca ggagtagagc gagacacaag gcaagggagg
     3481 gcaggagaaa atggcagcgt cgtccggctt cgtcgatccc tcgggttccg ggcgccagtg
     3541 gtacgacctc ttctctcgcg gcgcgcgcga ttggctgcgg cacaacgaca aggtgcgcgc
     3601 ggccgtccgc gacaagctcc ccgagctcat cgccggcagc gagctgctgc cctccgccga
     3661 ccgcaccgtc caagttcccg tccgcttcct cgagcacgcg cgcctgcggc tgcgtgacgc
     3721 cgagcaatcc gaaggcgtcg gtcaaggcgc cggcaagccc ggcgatcagc tcgtccagcc
     3781 gagcgacgga acgatgtcga agggcgcggg tggcaacgag gagggcggct accagttcat
     3841 cctcgagctc aagatcgacg acatcgtcga ctggctctgg gaggagctga agctgccgaa
     3901 cttgaaggtc aagtccggcg ccgttcagta cgacgactac acgcgcgaag gctggagccg
     3961 gcgcggcgtg cgctcgcggc tcgaccggcg ccgctcgctg aaggaagcga tcaagcgccg
     4021 cgccgtgcag gacgaaggcg ccgcgttcac gaacgacgac ttgcgctacc gccagctcgc
     4081 gatgcgccgg cggcccgcga ccgaagccgt cgtctatttc gcgatggacg tgtcgtcgag
     4141 catgaccgag aacgaccgca agctcgcgaa gacgttcttc ttctgggtcg tgcaggggct
     4201 gcgccgccaa tacacgcacc tcgagaccgt gttcgtcgcg cacacggtcg aggcgtggga
     4261 gttcgcggag caagagttct tccaggtcag cggcagcggc ggcaccgtcg cctcgacggc
     4321 gttccgcaaa gtgctcgaga tcgcgacgca ccgctacgat ccgggccgct tcaacacgta
     4381 cgtgttctac gcgtcggacg gcgcgaactt ccataacgac cgcgaggccg cgcaagccgc
     4441 gctgaacgag ctcggcgaga tcgcgaacta cgtcggctac gtcgagacgg cgtccgtcgc
     4501 gcagcagccg ctgcaatccg agacggcgct gttgttcgag cagctcgagg ccgcgacgca
     4561 tgccgcgggc cgccacgcgc tgtcctcgcc cgagtcggta tgggatgcga ttcgcgcgtt
     4621 cttcagccgg cgcgcggagg ccgaggtcta gcatgaacga ggcgctgcaa acctacgcgg
     4681 gccggctcga agctctcgca aagagccacg gcctcgacta ctaccccgtg cagttcgaag
     4741 aggtgccttc gagcttcatg atggagatcg ccgtgtacgg gctgcccgtg cgcatgccgc
     4801 actggtcgtt cggcgtccgc tacatctatc agctcgtcca gcaccgcatg ggccactcgc
     4861 ggctcttcga ggtcgtgttc cccggcaatc cgggccgcgc ttatctcgcc ggcaacaact
     4921 cgctgcaaga gaacacgctc gtcgcggcgc acgtgctcgg gcacgccgac ttcgcgaaga
     4981 acaactcgct gttcaagcgc agccaggagc aggtcggtta ccgcatcgtc gagcaggctg
     5041 ccgcgcatgc gcgccagatc ggcgcggccg tcgagcagta cggccagaat cgcgtcgagc
     5101 aagtgctcga cgcggcgctg tcgctcgaag cgcacatcga cccgatccag ctcctgcgcc
     5161 gcgatcggta cccgcaatac gtcgaagcgc cgcgcccggt gcaggagagc ggcttccaga
     5221 ggcggttcga gagcttgccg ggcagccccg aagtcgtcga cgcgccgctc gaacgcgcgc
     5281 gcgcgccgat cccggcggcg cccgagcgcg acttgctctg gctcatcgcc gagtacgcgc
     5341 ccgagctcga ggagtgggag cgcgacatct tccgcgccgt gcgcgaggag tcgttctatt
     5401 tctaccccgt gttcgcgtgc cagatcatga acgaaggctg ggcgtcgtat tggcacgctc
     5461 gcctgctgcg cgaggcggac ttcctgccgc agaacgagta tctcgacgcg atcaagacgc
     5521 actccgacgt cgtccggccg cacgcggcgg agcagcaagc gtcgctcgcg acgaatccgt
     5581 atcacttggg attctcgatc tgggagtcga tcgtcaagga gcacggcatc gagcgcgcgt
     5641 ttcagatccg cgccgaggac gacgacttca gcttcgtccg caatcacttg accgaagaac
     5701 tcgccgagga gctgaagctg ttccgctacg agcgcagcgc cgacggcggc gtcaaggtgc
     5761 tcgaccacga catacacgcg ctgcgcgaaa ccgtgctcgc gccgaagtac aacttcggcg
     5821 cgccggccgt tctcgcgaag cacatcgcgc acgacggcgc gctgacgctc gcccacgagc
     5881 acgacaccga cggccgcggc ctcgacttgg accgcgccga gcgcgtgctc gactacctgc
     5941 acaaggtctg gcgccgcccg atccggctcg agacgatcga ctcgtcgggc cagccgcgca
     6001 caatctcccg cgccgcctga aaaggcttgc tctgccatcg gcaggcagtg acaccccggt
     6061 gccggtgtta cggtttcggc tcaaccgaag tgccggagtt acggttcaac cgaagtgccg
     6121 gtgttacggt tcaaccgaac cgtaacaccg gcaccgcatt cagtcgcttt tgtcagtcgt
     6181 ccaggtcgct gagcgtgacc gtgatgtcct gcttcgagac gtcgacgttc cagaacgtgt
     6241 acttgtcgcc gtcttcgtcg acgagg
//
LOCUS       DASSNN010000735         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1873106, whole genome shotgun sequence.
ACCESSION   DASSNN010000735 DASSNN010000000
VERSION     DASSNN010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873106"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VFL84_13825"
     CDS             <1..1137
                     /locus_tag="VFL84_13825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HET7609754.1"
                     /translation="LDSRPIPRWRAEDLDPRVYALSRASQHLLAHVGAWSRIAETRAA
                     PYLRMRVWEGADAYAANALDFDSADIGEPDLGHIVEDALLRTVLADRLAASREAQLVI
                     GAEIESVAASEREIAVALKDGGSLRGAVLLAADGSDSTVRRLRGLPVAGHGYGQNAVV
                     THVTSTRGHQSTAWQRFLPGGPLALLPLADGRSSIVWSLPTLDAERLLAASDAEFLAE
                     LGAASLGCVGELTACSKRVAFPLQALHALKYTAPRVALLGDAAHTVHPLAGQGMNLGI
                     LDAASIAAVVEDALIAGEDIGDLKVLRRYERQRKGDNLAMLAAFDGLNRLFRLPSWAA
                     PMRELGLRAVESAAPVKRVLMQKALGLEAGRRLEVRWSRAELQA"
     gene            1149..>2463
                     /locus_tag="VFL84_13830"
     CDS             1149..>2463
                     /locus_tag="VFL84_13830"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7609755.1"
                     /translation="MSDIRTLSAGAFLGALVLLAAPLAAQEQTWNRTLERIATGVVSI
                     QIDSTRAFDTERNQSSQATGFVVDAERGLILTNRHVVTPGPVRAQAVFLNQEEVDLVP
                     VYRDPIHDFGFFRYDPADLQFMKPTELELDPTAAQIGRDIRVVGNDAGEQLSILAGTI
                     ARLDRQAPNYGRGNYNDFNTFYLQAASGTSGGSSGSPVVDIEGRVVALNAGANSQAAS
                     SFFLPLDRVEVALANIQAGKPVPRGTLETEFVHKPFAELRRLGLTRDTETRVRQTFPR
                     QTGLLVVEQVIPESAAASKLEPGDVLVEVDGKLIAEFVPLAAVLDGAVGREVSVVIER
                     GGQRLETRVKVDDLHEITPDEYIEYGDGVFHKLSYQQARHFYRPVAGVYVANPGYVFG
                     KAAIPRGSIVTGIGGEKLDDLDDLQRALESLPDDAQVPVRFVTFDE"
BASE COUNT          380 a          868 c          829 g          386 t
ORIGIN      
        1 ctcgactcgc ggccgatccc gcgctggcgc gccgaggacc tggatccgcg cgtctacgcg
       61 ctgtcgcgcg cgtcgcagca tttgctcgcg cacgtcggcg cgtggagcag aatcgccgag
      121 acacgcgccg cgccgtacct acgaatgcgc gtctgggaag gcgccgatgc ctacgcggcg
      181 aacgcactcg acttcgacag cgccgacatc ggcgagcccg atctcggcca catcgtcgag
      241 gacgcgctgc tgcgcaccgt gctcgccgac cggctcgccg cgtcgcgcga agcgcagctc
      301 gtcatcggcg ccgagatcga gtccgtcgcg gcgagcgagc gggaaatcgc cgtcgcgctg
      361 aaggacggcg gcagcttgcg cggcgccgtg ctgctcgcgg ccgacggcag cgactcgacg
      421 gtgcggcgcc tccgcggctt gcccgtcgcg ggtcacgggt acgggcagaa cgccgtcgtc
      481 acgcacgtga cgagcacgcg cggccaccag agcacggcgt ggcagcggtt cctgccgggc
      541 ggcccgctcg cgctgctgcc gctcgccgac gggcgcagct cgatcgtctg gtcgctgccg
      601 actttggatg cggagcggtt gctcgcggca agcgatgccg agtttctcgc cgagctcggc
      661 gcggcgagct taggatgcgt cggcgagctc acggcgtgct cgaagcgcgt cgcgttccct
      721 ctgcaagcgc tgcacgcgct gaagtacacg gccccgcgtg tcgcgctgct cggcgatgcc
      781 gcgcataccg tgcaccccct ggcggggcag ggcatgaact taggcattct tgatgcggcc
      841 tcgatcgcgg ccgtcgtcga ggacgcgctg atcgccggcg aagacatcgg cgacctcaaa
      901 gtgctgcgcc gctacgagcg gcagcgcaag ggcgacaacc tcgcgatgct ggccgctttc
      961 gacggcctga accgcttgtt tcgccttcct tcatgggccg cgccgatgcg cgagctgggc
     1021 ttgcgcgccg tcgaatcggc tgctcctgtc aaacgcgtct tgatgcaaaa ggcgctcggc
     1081 ctcgaagcgg ggcgtaggct cgaagtccgc tggtcacgcg cggagctgca agcatagaat
     1141 ccggcgctat gagcgatatt agaaccctgt ccgccggagc gtttctcggc gctctggtcc
     1201 tcttggctgc gccgctcgcg gcgcaagagc aaacctggaa tcgcacgctc gagcgcatcg
     1261 cgaccggcgt cgtgtcgatc caaatcgaca gcacgcgcgc gttcgacacc gagcgcaacc
     1321 aatcgagcca agcgacgggc ttcgtcgtcg acgccgagcg cggcttgatt ctgacgaacc
     1381 ggcacgtcgt gacgccgggc cccgtgcgcg cgcaagccgt cttcctgaac caggaggagg
     1441 tcgatctcgt ccccgtctat cgcgacccga tccacgactt cggcttcttc cgctacgacc
     1501 cggcggatct gcagttcatg aagccgaccg agctcgagct cgatcctacc gccgctcaga
     1561 tcggccgcga catccgcgtc gtcggcaacg acgccggcga gcagctctcg atcctcgccg
     1621 gcacgatcgc gcggctcgac cgccaagcgc cgaactacgg ccgcggcaat tacaacgact
     1681 tcaacacgtt ctatttgcaa gccgcgtcgg gcacgtcggg cggctcgtcg ggctcgcccg
     1741 tcgtcgacat cgaaggccgc gtcgtcgcgc tgaatgccgg cgccaactcg caagcggcgt
     1801 cgagcttctt cctgccgctc gaccgcgtcg aggtcgcgct cgcgaacatc caagccggaa
     1861 aaccggtgcc gcgcggcacg ctcgagaccg agttcgtgca caagccgttc gccgagctgc
     1921 gccgcctggg cttgacgcgc gacaccgaga cgcgcgtgcg ccagaccttt ccgcggcaga
     1981 cgggtctgct cgtcgtcgag caggtgattc cggagtcggc cgcggcgagc aagctcgagc
     2041 ccggcgacgt tctggtcgaa gtggacggca agctcatcgc ggagttcgtt ccgctcgcag
     2101 cggttttgga cggcgcagtg ggccgcgagg tcagcgtcgt catcgagcgc ggcgggcagc
     2161 ggctcgaaac ccgcgtcaag gtcgacgatc tgcacgagat cacgcccgac gagtacatcg
     2221 agtacggcga cggcgtcttt cacaagctct cgtatcagca agcgcggcac ttctatcggc
     2281 ccgtcgcggg cgtctacgtc gcgaatcccg gctacgtctt cggcaaggcc gcgatcccgc
     2341 gcggcagcat cgtgaccggc atcggcggcg agaagctcga cgacctcgac gaccttcagc
     2401 gagccctcga gtcgttgccc gacgacgcgc aggtgcccgt gcgcttcgtg acgttcgacg
     2461 agc
//
LOCUS       DASSNN010000736         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_988440, whole genome shotgun sequence.
ACCESSION   DASSNN010000736 DASSNN010000000
VERSION     DASSNN010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988440"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFL84_13835"
     CDS             complement(<1..191)
                     /locus_tag="VFL84_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609756.1"
                     /translation="MRGNGHKETTISALTWKTSPRWYLWEGGFLLLGAAQGVVPLHAH
                     HAIQIVIAIDGEVAVKEERG"
     gene            complement(249..887)
                     /locus_tag="VFL84_13840"
     CDS             complement(249..887)
                     /locus_tag="VFL84_13840"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7609757.1"
                     /translation="MSNVRAPAVLGRAVRQRRSQKTYDALISTGFKLLEKREFDSITI
                     AALAQAAGYSVGAFYSRFQSKDEFFEALVAHHLEERARARGALLESSASEDWLEALIA
                     DIANYYWRRRRFWRAALVRSGHDPAFWEPIRRHARDNYESLVARIQRDARRALSKEEC
                     TNIRFAFQLVLSAVNNRIMNRPRPSFIGQADFIAFLVRAFRLVSEYDKLVGA"
     gene            1032..1622
                     /gene="pnuC"
                     /locus_tag="VFL84_13845"
     CDS             1032..1622
                     /gene="pnuC"
                     /locus_tag="VFL84_13845"
                     /inference="COORDINATES: protein motif:HMM:NF016837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HET7609758.1"
                     /translation="MTWTETIGFALGAWCVWLIVKEHIWNWPVGIAQCAFYIVVFWRA
                     GLYADALLQIVYIALGVLGWYWWLRGGSARTALRIERVPAIALLPLVAVTVVGTGLLS
                     AVLARATNSTVPFWDALTTVLSLTAQFMLSRKWLENWYVWIAADVVYIGLYLYKGLLL
                     TTALYAGFMALCVAGLLRWRGTMSSRTEVPAKVLSP"
     gene            1619..>2439
                     /locus_tag="VFL84_13850"
     CDS             1619..>2439
                     /locus_tag="VFL84_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7609759.1"
                     /translation="MSARFGRGLVLGKFYPPHAGHSYLISSARAKCERLTVMVLAAHV
                     ESIPLEERRAWLAELHPDCEIVAGYDDVPMDIEDADIWARHMEAIEALLDEQADAVFT
                     SEPYGDELARRLGAVHVSVDPARTAVPCSGTAVRADPARYWWALAPPVRAWFARRVVV
                     VGAESTGTTTLAAALAHHYSTLWVPEAGRDWSASRPGGLSARWRSEEFDEIARRQAAS
                     EDEAARKLPRPLLFCDTDALATAVWHERYMGRRSPNVEAFAAARVPDLYLLTLDD"
BASE COUNT          396 a          828 c          779 g          436 t
ORIGIN      
        1 ccgcgctcct ccttcaccgc aacctcgccg tcgatcgcga tgacgatctg aattgcgtga
       61 tgggcgtgca aaggaaccac gccttgcgcc gcgccgagca ggaggaagcc gccttcccaa
      121 agataccacc gcggcgacgt cttccaggtc agtgcggaga tcgttgtttc cttgtgcccg
      181 tttccccgca tcggcgccgc tccgttacac gcgatcgatc cacgagctcc agtcgcgcgg
      241 ttcggccctc atgctccgac gagcttgtcg tactccgaca cgagccgaaa cgcgcgcacg
      301 agaaaggcga tgaagtcggc ctgcccgatg aacgagggcc gcggccgatt catgatgcgg
      361 ttattgaccg cgctcagcac cagctggaac gcgaaacgaa tgttcgtgca ctcctccttg
      421 ctcagcgctc gccgcgcgtc ccgttgtatg cgggcgacca gcgactcgta gttatcccgc
      481 gcatgtcgcc tgatcggctc ccagaacgcg ggatcgtgcc cgcttcgcac gagcgcggcg
      541 cgccagaacc gccgcctccg ccagtagtag ttggcgatgt cggcgatgag cgcctccagc
      601 cagtcttcgc tcgccgagct ttcgagcagc gccccgcggg cccgagcgcg ctcctcgagg
      661 tggtgggcga ctaaagcctc gaagaactcg tccttgcttt gaaaacggga ataaaacgcc
      721 ccgaccgaat agcctgccgc ctgggcgagc gccgcgatcg tgatggagtc gaactcgcgt
      781 ttctcgagaa gtttaaagcc cgtggagatc agggcgtcgt aggtcttctg gctccgcctt
      841 tgcctgactg cccgtcccag gacggccggc gcacgaacgt tgctcatggg tcaacaaccg
      901 ggtccgctcg aagcggcgac tatacaacgg cgcacgcttg gatgccgagc gcagcactta
      961 tactccacgc ctcgaaacaa aacggcggac ccgacgggcg ggtcggcccg taaagcgcga
     1021 gcaagccatc catgacgtgg accgaaacga tagggttcgc cctcggcgcc tggtgcgtct
     1081 ggctcatcgt gaaagagcac atttggaact ggccggtcgg catcgctcag tgcgccttct
     1141 atatcgtcgt gttctggcgc gccggcctct acgcggatgc cctgctgcag atcgtctaca
     1201 tcgcgctcgg cgtgcttgga tggtactggt ggctgcgcgg cggcagcgcg cgcacggcac
     1261 tgcggatcga gcgcgtgccg gcgatcgcac tgctgccgct cgtcgcggtt acggtcgtcg
     1321 gtaccggcct tctttcggcg gtcctcgcgc gcgccacgaa ttcgaccgtg cccttctggg
     1381 acgcgctgac gaccgtcttg agcctgaccg cgcaattcat gctgagccgc aagtggctcg
     1441 agaactggta tgtctggatc gctgccgacg tcgtctacat cgggctgtac ctctacaaag
     1501 gactgctcct gacgaccgcg ctctacgccg gcttcatggc cctgtgcgtg gcgggcctgc
     1561 tgcggtggcg gggcacgatg agctcccgga cggaagtccc tgcgaaagtg ctcagtccgt
     1621 gagcgcgcgc ttcggccgag gcctcgtgct cggcaaattc tatccgccgc atgccggcca
     1681 ttcatacctc atctccagcg cccgggctaa atgcgaacga ttgacggtca tggtgctcgc
     1741 cgcgcacgtc gaatcgattc cgctcgaaga gaggcgcgcc tggctcgcag agctgcatcc
     1801 cgattgcgag atcgtcgcgg gctacgacga cgtccctatg gatatcgagg acgccgatat
     1861 ttgggcgcgg cacatggaag cgatcgaagc gctgctcgac gaacaggccg atgcagtctt
     1921 tacatccgag ccttacggcg acgagctcgc gcgacggctc ggcgccgtgc atgtctccgt
     1981 cgatccggcg cgaaccgccg taccgtgcag cggcacggcc gtccgcgccg acccggcacg
     2041 ctactggtgg gccctcgcgc cgcccgtccg agcttggttc gcgcggcgcg tcgtcgtggt
     2101 cggcgcggaa tcgacgggca ccactacgct cgccgcggcg ctcgcgcatc actactcgac
     2161 gttatgggtg ccggaagccg gccgcgattg gtcggcgtcg cgcccgggcg gactctcggc
     2221 tcgctggcgc agcgaagagt tcgacgagat cgcccgccgg caggccgcga gcgaggacga
     2281 ggccgctcga aagcttccac ggcccctact cttctgcgat accgatgcgc tcgcgacagc
     2341 ggtttggcac gagcgataca tgggccggcg ctcgccgaat gtcgaggcgt tcgccgccgc
     2401 gcgcgtcccc gatctctact tgctgacgct cgacgacat
//
LOCUS       DASSNN010000737         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_699356, whole genome shotgun sequence.
ACCESSION   DASSNN010000737 DASSNN010000000
VERSION     DASSNN010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699356"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFL84_13855"
     CDS             complement(<1..309)
                     /locus_tag="VFL84_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7609760.1"
                     /translation="MDKPRHAFRHLDLKHLRQLTEVADKGSIRAAAESLAITQPALSR
                     SIRSIENELGLKLIERGPRGAELTPAGARLLSYARIIDANLTSAENELRGLRDRSSRT
                     E"
     gene            387..911
                     /locus_tag="VFL84_13860"
     CDS             387..911
                     /locus_tag="VFL84_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HET7609761.1"
                     /translation="MSFERRGIGEFATAFGSLASYVKGDIEIINDDVKSYAFSNVFDV
                     AAHAAPYEKIVVAKNRQYVIETLRAEGTSDWFSASHDEFAIVMDGRVEIELVKLDFPG
                     TVVPPDVEGSVKLASAPVGRNMGFMRLGRGHQALLPPRAAYRFRSTAGVGVILLQTIL
                     GPHSVQKWSAICRT"
     gene            908..1930
                     /locus_tag="VFL84_13865"
     CDS             908..1930
                     /locus_tag="VFL84_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HET7609762.1"
                     /translation="MNDSAVRASAPNEAGYRSFRLGSFTFSRDEYFAHVEWISKGTKL
                     SHTMSADVFLRALMRDVAWNFFYGTVNFDDVFGTRNRYGLVEFFAGCYHPTYKSQGLD
                     LLETFDSGLAMQTCKAILHDWTNLGYDPFAAPMETGLSWIGKKVGHNSAAIDRARDAC
                     KRMPGLPGDLPLRTDRNRYPVNRAFLDVPQDEPEIHAAPGFEGEVHAFNLFAYLSRSD
                     VTWNPSVSSVCKHSLFCPTTEEYILPVKHANDRVEWFLQLSDQIEWQIEDGVTGLPRA
                     KVVMRPGDVAAMPADIRHQGFARKRAMLLVWENADPELPRLHAQGAVPRSPAEEFWPE
                     PTRQQR"
     gene            2033..>2511
                     /locus_tag="VFL84_13870"
     CDS             2033..>2511
                     /locus_tag="VFL84_13870"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET7609763.1"
                     /translation="MIGHSQRLVAAAVAAALVPSARVLAQSEGLEEILVTAQRREQSL
                     QDVPISVTAITGEAIEEGGFSDVEDLSIFVPNLFMRDAFTGQSLIVRGVGTSTGNEAF
                     EQAVAAFSDGVYYGRDNLSQGAVFDLERVEVVRGPQPTFAGQSATAGAINVITRKPG"
BASE COUNT          430 a          813 c          805 g          463 t
ORIGIN      
        1 ctccgtgcgg ctgctgcgat cgcgtaagcc ccgcagctcg ttctcggcgg aggtcaggtt
       61 ggcgtcgatg attcgcgcgt agctcaggag ccgggctccc gcaggagtga gctccgcgcc
      121 gcgcggccct cgttctatca gcttcaatcc gagctcgttc tcgatgctgc ggatactgcg
      181 ggacagcgcg ggctgggtga tcgcaagcga ctcggccgct gcgcggatgc tgcccttgtc
      241 cgctacttcc gtcagctgcc gcaaatgctt gaggtccaag tgacggaaag catgcctcgg
      301 cttgtccaag acgtgatcct aaggtagcga ctgccctcgg cagtatactc acggcacggt
      361 acttccagcg gggggcgaca gcgcgaatga gctttgagcg gcgcggaata ggcgagttcg
      421 ccaccgcctt cggttcgctc gcgagctacg tcaaaggcga catcgaaatc atcaacgatg
      481 acgtcaagag ctacgcgttc tcgaacgtgt tcgacgtcgc tgcgcacgcc gccccgtacg
      541 aaaagatcgt cgtcgccaag aatcgccaat acgtgatcga gacgctgcgc gcggagggca
      601 cgtcagattg gttctccgcg tcgcacgacg agtttgcgat cgtgatggac ggccgcgtcg
      661 agatcgagct cgtgaagctc gattttcccg gcacggtcgt gcctcccgac gtcgaagggt
      721 cggtcaagct cgcaagcgcg ccggtcggcc gcaacatggg cttcatgcgg ctcggccgcg
      781 gtcatcaggc gctgttgccg ccgcgtgccg cataccggtt ccgctcgacg gccggggtcg
      841 gcgtcatcct gctgcagacg atcctcgggc cgcattccgt gcagaagtgg tcggcgatat
      901 gccgcacgtg aacgactcgg cggttcgagc gtccgcgccg aacgaggccg gctaccgcag
      961 ctttcggttg ggctcgttca cgttctcgcg cgacgaatac ttcgcgcacg tcgagtggat
     1021 ctcgaagggc acgaagctct cgcacacgat gtcggccgac gtgttcttgc gcgcgctcat
     1081 gcgcgacgtc gcgtggaatt ttttctacgg cacggtgaat ttcgacgatg tgttcggcac
     1141 tcggaaccgc tacggtctcg tcgaattctt cgccggctgt taccacccga cctacaagtc
     1201 gcagggcctc gatctcctcg agacgttcga cagcggtctc gcgatgcaga cctgcaaggc
     1261 gatcctgcac gactggacga atttagggta cgacccgttc gcggcgccga tggagacggg
     1321 gctgtcctgg ataggcaaga aggtcggaca caactcggcc gcgatcgatc gtgcgcgcga
     1381 tgcctgcaag cgcatgcccg gcttgcccgg cgacctgccg ctgcgcaccg accgcaaccg
     1441 ctatcccgtc aaccgcgcgt tcctcgacgt gccgcaggac gagcccgaga ttcacgccgc
     1501 gccgggcttc gaaggcgaag tgcacgcgtt caacctgttt gcctatctgt cgcgttccga
     1561 cgtgacctgg aacccgtccg tgagctcggt ctgcaagcac agcctgtttt gcccgacgac
     1621 cgaggaatac atcctgccgg tcaagcacgc caacgaccgc gtcgaatggt ttctgcagct
     1681 gagcgaccag atcgaatggc agatcgagga cggcgtgacg gggttgccgc gcgccaaggt
     1741 cgtcatgcgc cccggcgacg tcgcggcgat gcccgccgat attcggcatc aaggatttgc
     1801 gcgcaagcgc gccatgttgc tcgtctggga gaacgccgac cccgagttac cgcgcctgca
     1861 tgcgcaaggc gccgtcccga ggagcccggc cgaggaattc tggcccgagc cgacccggca
     1921 gcaacgctga ggcgtttcgt tcccccgagg caagcgggtc gtcttgtcgt cgggacgacc
     1981 cctcctgcgt actactagct agctggaatc aattttcccg ggggggatcg agatgattgg
     2041 ccattcccag cgtttggtcg cggccgccgt cgccgcggca ttggtaccga gcgcccgtgt
     2101 gctcgcgcaa agcgaaggtc tcgaagagat tctcgtcacg gcacaacgtc gcgagcagag
     2161 ccttcaggac gtcccgattt cggtcaccgc gatcaccggt gaggccatcg aggagggcgg
     2221 tttctccgat gtcgaagacc tgtcgatttt cgtgccgaac ttgttcatgc gcgacgcctt
     2281 cacggggcaa tcgctgatcg ttcgcggcgt cggcacgtcg accggcaacg aagcgttcga
     2341 gcaagccgtc gccgcattct cggacggcgt ctactacggg cgcgacaact tgagccaagg
     2401 cgccgtgttc gatttggagc gcgtcgaggt cgtacgaggg ccgcagccca cctttgccgg
     2461 ccaaagcgcc accgccggcg cgatcaacgt catcacgcgc aagcccggcc a
//
LOCUS       DASSNN010000738         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_125970, whole genome shotgun sequence.
ACCESSION   DASSNN010000738 DASSNN010000000
VERSION     DASSNN010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125970"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFL84_13875"
     CDS             <1..760
                     /locus_tag="VFL84_13875"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HET7609764.1"
                     /translation="FIDSVLRSDQSVVDLLTADYTYLNERLAMHYGIETVKGARFRRV
                     TLPDDSRYGLLGKGAVLALTSYPNRTSPVLRGQWILARLVGAPPPAPPPDVPDLAENK
                     RGEPPRTLRARLEQHRENPTCFACHGIMDPLGFALENYNAVGQYRSLDPDTRTPVDAS
                     GVLPDGTQVSGVGDLRRALTARPEQFVQALTENLMTFALGRTLDYRDMPTVRAIVRNA
                     ERDDYRFESLVLGIVSSDAFREREAVTETVASLE"
     gene            789..>2046
                     /locus_tag="VFL84_13880"
     CDS             789..>2046
                     /locus_tag="VFL84_13880"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET7609765.1"
                     /translation="MFLTRKHLSRRTVLKGAGASIALPLLDAMIPAGTALAKTAAAPA
                     TRLGFVYFPHGALRDQWEPATTGRNPKYPFILEPLEPVRDYVTIVSGLRNKAGEGGVP
                     HGITEETWLNCVHPRQRNVDKGIGVTADQLAARYLQKDTPLPSMEICGEPGGMISFRT
                     PNQPLPMEGNPRKVFISMFGQGQTNAERQTILGTTTSLLDYVTEASASLNRKLDAADR
                     AKVEDYLDSVREVEQRVEKLKQSSESLANLPDAPIGAPDDFGELLDVQFELFALAWET
                     NRTNVVTMKMVEEASMRVYANLGVHDAFHPTSHWGGFPERIANLRKIQNYHTQIFSKF
                     AQRLARTPDGDGSVLDHSIILFGSNMGNSDAHDADLLPEALIGRGGGLKGNQHLHYPR
                     NTPLANLLVTMLDRAGIPGNEIEKFGD"
BASE COUNT          364 a          709 c          667 g          306 t
ORIGIN      
        1 cttcatcgac agcgtgctgc gcagcgatca gagcgtcgtc gacctgctga ccgcggacta
       61 cacgtatctg aacgagcggc tcgcgatgca ctacggcatc gagaccgtca aaggcgcgcg
      121 cttccgccgc gtcacgctgc ccgacgactc gcgttatggg ctgctcggca agggcgccgt
      181 cctcgctctg acgtcgtatc cgaaccgaac ctcgccggtg ctgcgcggcc agtggatcct
      241 cgcgcgcctc gtcggcgctc cgccgccggc gccgccgccc gacgttccgg acctcgccga
      301 gaacaagcgc ggcgagccgc cgcgcacgct gcgcgcgcgg ctcgagcagc atcgcgagaa
      361 tccgacctgc ttcgcgtgcc acggcatcat ggatcccctc ggcttcgcgc tcgagaacta
      421 caacgccgtc ggtcagtacc gctcgctcga tcccgacacg cgtacgcccg tcgatgcgag
      481 cggcgtgctg ccggacggaa cgcaagtgag cggcgtcggc gacttgcgcc gcgcgctgac
      541 ggcgcgcccc gagcaattcg tgcaagcgct gaccgagaac ctcatgacgt tcgcgctcgg
      601 ccgcacgctc gactatcgcg acatgccgac ggtccgtgcg atcgtccgga acgcggaaag
      661 ggacgactat cgcttcgagt cgctggtgtt gggcatcgtc tcgagcgatg ccttccgcga
      721 gcgggaggcc gtcaccgaaa cggtggcgtc tttggagtaa gctaagaggg aaacggagag
      781 ctgccgctat gttcttgacg cgcaagcatc tgtcacggcg taccgtcctg aaaggggccg
      841 gcgcctcgat cgcgctgccg ctgctcgatg cgatgatccc ggccggtacc gcgctcgcga
      901 agacggccgc cgcacccgcg acgcggctcg gcttcgtgta tttcccgcac ggcgcgctgc
      961 gcgaccagtg ggagcccgcc acgacgggcc gcaacccgaa atatccgttc atcttggagc
     1021 cgctcgagcc cgtccgcgac tacgtcacga tcgtcagcgg cctgcgcaac aaggccggcg
     1081 agggcggcgt gccgcacggc atcaccgagg agacctggct caactgcgtg catccgcgcc
     1141 agcgcaacgt cgacaagggc atcggcgtca cggcggacca gctcgcggcg cgctacttgc
     1201 agaaggacac gccgctgccg tcgatggaga tctgcggcga gccgggcggc atgatctcgt
     1261 tccgtacgcc gaaccagccg ctgccgatgg aaggcaatcc gcgcaaggtg ttcatctcga
     1321 tgttcggcca gggccagacg aacgcggaac gccagaccat cctcggcacg acgacgagct
     1381 tgctcgacta cgtgacggag gcgtcggcca gcctgaaccg taagctagat gccgccgacc
     1441 gcgcgaaggt cgaggactat ctcgactccg ttcgcgaggt cgagcagcgc gtcgagaagc
     1501 tcaagcagag cagcgaatct ttggcgaact tgccggatgc gccgatcggc gcgccggacg
     1561 acttcggcga gctgctcgac gtgcagttcg agctgttcgc gctcgcgtgg gagacgaacc
     1621 gcacgaacgt cgtcacgatg aagatggtcg aggaagcgag catgcgcgtg tacgcgaacc
     1681 tcggcgtcca cgacgcgttc cacccgacgt cgcactgggg tgggttcccg gagcgcatcg
     1741 cgaacctgcg caagatccag aactatcaca cgcagatctt ctcgaagttc gcgcaacgcc
     1801 ttgcgcgcac gccggacggc gacggctcgg tgctcgacca ctcgatcatt ctgttcggca
     1861 gcaacatggg caacagcgac gcgcacgacg cggacctgct gcccgaagcg ctgatcgggc
     1921 gcggcggcgg cctcaagggc aatcagcacc tgcactatcc gcgcaacacg ccgctcgcga
     1981 acttgctcgt cacgatgctc gaccgcgccg gcatccccgg caacgagatc gagaagttcg
     2041 gcgaca
//
LOCUS       DASSNN010000739         6060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_700079, whole genome shotgun sequence.
ACCESSION   DASSNN010000739 DASSNN010000000
VERSION     DASSNN010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700079"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1341
                     /gene="lysA"
                     /locus_tag="VFL84_13885"
     CDS             91..1341
                     /gene="lysA"
                     /locus_tag="VFL84_13885"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET7609766.1"
                     /translation="MRQLAGRWRELVAHERKFAGGVFGETWERLTVGAPPARPAPKPW
                     WVKKKAQLLEIGAQRDATYVYDRESIEQAVAALRSLKSVDAVFYAMKANPNREILKLV
                     DAGGLNFECVSPGEIARVLEVVPRIDRKRILFTPNFAPRSEYEQALEQGVWVTLDNLF
                     PLKEWPKSFKGKEVFVRIDTGQGRGHHEHVRTAGVHSKFGIPLFEIDELVEAAKRAGA
                     RIVGLHAHTGSGVLAASAWLDTGRQLLKLLEKLPDVRYIDVGGGLGVPEKAGQPPFDL
                     QALDTALAEIKQGCGGRSLFLEPGRFIVAQAGVIVARVTQTKGKGDVQYVGVGTGMNS
                     LIRPALYGAYHEIVNLTRLGLPATELVTVVGPICETGDRLGSDRLLPAAKENDVLLIA
                     NAGAYGYAMSSRYNLREPAQEVLI"
     gene            1352..2176
                     /locus_tag="VFL84_13890"
     CDS             1352..2176
                     /locus_tag="VFL84_13890"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7609767.1"
                     /translation="MHGPKNSLARVLYRFGTGRRLARNEWAAPYWEERDGQRVVACRL
                     CPKLDRDRGECSVPFGTPLRKCVVAATEAHLRDTRGARVLEIGYARHSYGKRIVEHCG
                     GSWTGIEPLVSRSRAPNVGAGSYGHAGEIPFQDETFDRVFGNQTFEHWGEPLPDGTAS
                     PGYRACLAEIWRVLKLGGRLYLDAPIHLHGHEMFVAGDLGRILALFDGESWSDIVVER
                     WRYDHSPLPRYPTPEGDARDALLNVPSYDPAVIADVQRNGTVWLLTVAATKTGRAP"
     gene            complement(2187..2951)
                     /locus_tag="VFL84_13895"
     CDS             complement(2187..2951)
                     /locus_tag="VFL84_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET7609768.1"
                     /translation="MNGHASAGAGHRAHGAADARTIVERIALYDWSAAEAALDEYGWA
                     PLAELLTAAECDALAALYPFEQHFRSRVAMARHGFGRGEYKYFRYPLPTLVAELRTSL
                     YARLVGVANRWNELMRVDVRFPDRHEEFVERCHRAGQQRPTPLLLQYGPEDYNCLHQD
                     LYGEHVFPLQAVFLLSEPADDFAGGEFVLTEQRPRMQSRAEVVPLAKGDGAIFAVNQR
                     PKRGARGFYRVTMRHGASRVRSGRRYTAGVIFHDAR"
     gene            complement(3002..4060)
                     /locus_tag="VFL84_13900"
     CDS             complement(3002..4060)
                     /locus_tag="VFL84_13900"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7609769.1"
                     /translation="MTMNRRELLAGAGALAAAVAARGEAQTQSRAKVVDVHTHMYSHG
                     WVKAVEQAADPTFRIGDNGALIYRGGSIGRMTPPMLDWDLRIKDMDDAKVDVALISLT
                     APNVYWGTRAQSVAAARAINDDFLAAQRKYDGRIRWFASLPFQYEADALAELRRAKDN
                     GAIGVCTLTNILGTALTEPQYRAIWRECAAMQLPVFIHPTTPFTDGMGLADFGLGNTI
                     GFTSDTSLCFAKLILAGVLDELPDLKLIACHGGGAFPYLAARFDIMWQRTTSARKNEA
                     PPSTYLKRIWYDSIVYDQPTLDFLVERVGAERVLYGSDYPFTIGDMKGILARVDSLPP
                     HDRDAIRSGNAERLFDLG"
     gene            complement(4110..4811)
                     /locus_tag="VFL84_13905"
     CDS             complement(4110..4811)
                     /locus_tag="VFL84_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7609770.1"
                     /translation="MSDSLPTVLIPGLLCTPRLYAEQIPKVWRFGAVTVADHTRDDSM
                     GGIARRILAQAPPRFALIGLSMGGYVAFEVERQAPDRVAKLALLDTTARPDAPEQTEQ
                     RKRQIEMARNGRFDGIAQLLFPRFVAAARHGDRALQAVVRTMADDTGPEAFVRQQTAI
                     MNRVDSRPGLAAIRCPTLVLVGADDVLTPPDRAAEMAAAIPGARQVVVPACGHLSPLE
                     QPQAVTDALVAFLSS"
     gene            complement(4811..5104)
                     /gene="hisE"
                     /locus_tag="VFL84_13910"
     CDS             complement(4811..5104)
                     /gene="hisE"
                     /locus_tag="VFL84_13910"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HET7609771.1"
                     /translation="MSDTEFLAKLQQVIRQRLADAPEGSYTAKLASQGMLKVAQKLGE
                     EGVELALAAAAQDQARVTAEAADLVYHLLLLLALRDLTLADVVAELERRHRPR"
     gene            complement(5101..5886)
                     /gene="hisF"
                     /locus_tag="VFL84_13915"
     CDS             complement(5101..5886)
                     /gene="hisF"
                     /locus_tag="VFL84_13915"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HET7609772.1"
                     /translation="MLTRRIIPCLDVRDGVVVKGVQFRDHKVVGDIMTLAARYRDEGA
                     DELVFYDITASPDRRTVDRTWVNRIAAVLDIPFCVAGGIRSVADAETVLNLGADKISV
                     NSPALERPDLIDELVRRFGSQCVVIGVDTLTRDGDYHVYQYTGDPTRSRSAERRTLDW
                     VREVQDRGAGEIVLNCMNQDGVRQGYDVPQLAAVRAICQVPLIASGGAGAREHFAEVF
                     NRARVDGALAASVFHTATIEIGELKRYLAAAGIAIRPVDEEAP"
     gene            complement(5868..>6060)
                     /locus_tag="VFL84_13920"
     CDS             complement(5868..>6060)
                     /locus_tag="VFL84_13920"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HET7609773.1"
                     /translation="NVELYRECVRRWPGIEFQASGGVRDAGDLAALAATGVAATVSGK
                     ALLEGRLKPEEIRPFLRGA"
BASE COUNT          952 a         2041 c         2124 g          943 t
ORIGIN      
        1 ggccagcacg aacaccaggc gttgcttgat cgcgcgcagc aactgcaatt gctggacgcg
       61 ttcggcggtt tgcaggacga ggccgcgcgc gtgcgtcaac tcgcaggccg gtggcgcgaa
      121 ctcgtcgcgc acgaacgcaa gttcgcgggc ggcgtcttcg gcgaaacctg ggagcggctc
      181 accgtcggcg ccccgcccgc aaggcccgcg ccgaagccgt ggtgggtcaa gaagaaggcg
      241 cagctgctcg agatcggcgc gcaacgcgac gcgacctacg tctacgatcg cgaaagcatc
      301 gagcaagccg tcgccgcgtt gcgttcgctg aagtccgtcg atgccgtctt ttacgcgatg
      361 aaggcgaatc cgaatcgcga gatcttgaag ctcgtcgacg cgggcgggct caatttcgaa
      421 tgcgtctcgc cgggcgagat cgcgcgcgtg ctcgaggtcg tgccgcgcat cgaccgcaag
      481 cgaattctct tcacgccgaa cttcgcgccg cgctcggagt acgagcaggc gctcgagcag
      541 ggcgtctggg tcacgctcga caatctgttc ccgctcaagg agtggccgaa gagcttcaag
      601 ggcaaggagg tgttcgttcg catcgacacg ggtcaaggcc gcggtcacca cgagcacgtt
      661 cgcacggccg gcgtgcactc gaagttcggc atcccgttgt tcgagatcga cgagctcgtc
      721 gaagcggcga agagggccgg cgcgcgtatc gtcggcctgc acgcgcacac gggcagcggc
      781 gtgctcgcgg cgagcgcctg gctcgacacg ggccggcagc tgctgaagct gctcgagaag
      841 ctgcccgacg tgcgctatat cgacgtcggc ggcggtctcg gcgtgcccga gaaggcggga
      901 caaccgccgt tcgacttgca ggcgctcgac acggcgctcg ccgagatcaa gcaaggctgc
      961 ggcggccgct cgctgttcct cgagcccgga cgcttcatcg tcgcgcaggc gggcgtcatc
     1021 gtcgcgcgcg tcacgcagac gaagggcaag ggcgacgtgc agtacgtcgg cgtcggcacg
     1081 ggcatgaaca gcttgatccg gcccgcgctg tacggcgctt atcacgagat cgtgaacctg
     1141 acgcggcttg ggttacctgc gacggagctc gtgacggtcg tcggcccgat ctgcgagacc
     1201 ggcgatcggc tcggctccga ccggctgctg ccggccgcga aggagaacga cgtgctgctg
     1261 atcgcgaacg ccggcgccta tggctatgcg atgagctcgc gctacaacct gcgcgagccg
     1321 gcgcaagaag tgctgatcta ggaacgccca agtgcacggc ccgaagaatt cgctggcccg
     1381 tgtcttgtac cgcttcggca ccggacggcg gctcgcgcgc aacgagtggg ccgcgccgta
     1441 ctgggaagag cgcgacggcc agcgcgtcgt cgcgtgccgc ctgtgcccga agctcgatcg
     1501 cgaccgcggc gaatgcagcg ttccgttcgg cacgccgctg cgcaaatgcg tggtcgccgc
     1561 gaccgaggcg cacttgcgcg acacgcgcgg cgcgcgcgtg ctcgagatcg gttatgcgcg
     1621 ccactcgtac ggcaagcgca tcgtcgagca ttgcggcggc tcatggaccg gcatcgagcc
     1681 gctcgtctct cgcagccgcg ccccgaacgt gggcgccggc agctatgggc acgccggcga
     1741 aattccgttt caggacgaga cgttcgaccg cgtgttcggc aaccagacgt tcgagcactg
     1801 gggcgagccg ctgcccgacg gcacggcctc tcccggctat cgcgcatgcc tggccgagat
     1861 ctggcgcgtg ctgaagctgg gcggccggct ctatctcgac gcgccgatcc atctgcacgg
     1921 gcacgagatg ttcgtggccg gcgacctcgg acgcattctc gcgttgttcg acggcgagtc
     1981 gtggtccgac atcgtcgtgg aacgctggcg ctacgaccac agcccgctgc cgcgctatcc
     2041 gacgcccgag ggcgacgcgc gcgacgcgct gctgaacgtg ccgagctacg acccggccgt
     2101 catcgccgac gtgcagcgca acggcacggt gtggctgctg accgtcgccg cgacgaagac
     2161 cggccgcgcg ccgtagggcc caaggtctag cgggcgtcgt ggaagatcac gccggccgtg
     2221 taacgccgtc cggagcgaac tcggctcgcg ccgtgccgca tcgtcacgcg atagaagccg
     2281 cgcgcgccgc gcttcggacg ttggttgacg gcgaagattg cgccgtcacc cttcgcgagc
     2341 ggcactacct cggctcgcga ctgcatgcgc ggccgctgct ccgtgagcac gaattcgccg
     2401 ccggcgaagt cgtccgccgg ctcggatagc aggaacaccg cctgcagcgg aaatacgtgc
     2461 tctccataga gatcctggtg gagacagttg taatcctcgg ggccgtactg gagcagcagc
     2521 ggcgtcggtc gctgttggcc cgcgcgatgg cagcgctcga cgaattcctc gtgacgatcc
     2581 gggaagcgca cgtcgacacg catgagctcg ttccagcggt tcgcgacgcc cacgagccgc
     2641 gcatagagcg atgtgcgcag ctcggcgacg agcgttggca acggataacg aaaatatttg
     2701 tactcgccgc ggccgaaccc gtgccgcgcc atcgcgacgc ggctccggaa gtgctgttcg
     2761 aacggataga gcgccgccag tgcatcgcac tcggccgccg tcagcagctc cgcgagcggc
     2821 gcccagccgt attcgtcgag agccgcctcg gccgccgacc agtcgtagag agcgatgcgc
     2881 tcgacaatcg tgcgagcgtc ggcggcgccg tgcgctcgat gaccagcacc cgcggaagcg
     2941 tggccgttca tggagaaccc gtggagctca taccggccta ggactcaagg cttcgcgagg
     3001 gtcagccgag gtcgaacagc cgctccgcgt tgccgctgcg gatcgcgtcg cggtcgtgtg
     3061 gcggcaggct gtcgacgcgc gcgaggatgc ccttcatgtc gccgatcgtg aacggatagt
     3121 cggagccgta gagcacgcgc tccgcgccga cacgctcgac gaggaagtcg agcgtcggct
     3181 ggtcgtagac gatcgagtcg taccagatcc gcttcaggta cgtcgacggc ggcgcctcgt
     3241 ttttgcgcgc gctcgtcgtg cgctgccaca tgatgtcgaa gcgcgcagcg aggtacggga
     3301 acgcaccgcc gccgtggcac gcgatcagct taagatccgg cagctcgtcg agcacgcccg
     3361 cgaggatcaa tttcgcgaag cagaggctcg tatcggacgt gaagccgatc gtgttgccga
     3421 ggccgaagtc cgcgaggccc atgccgtccg tgaacggcgt cgtgggatga atgaacacgg
     3481 gcagctgcat cgcggcgcac tcgcgccata tcgcccgata ctgcggctcg gtcagcgccg
     3541 tgccgaggat gttcgtcagc gtgcacacgc cgatcgcgcc gttgtccttg gcccggcgca
     3601 gctcggcgag ggcgtctgct tcgtattgga acggcagcga cgcgaaccag cggatgcggc
     3661 cgtcgtactt gcgctgcgcg gccaagaagt cgtcgttgat cgcgcgggcc gccgcgacgc
     3721 tctgcgcgcg cgtgccccag tagacgttcg gcgcggtcaa gctgatcagc gcgacgtcga
     3781 ccttcgcgtc gtccatgtcc ttgatgcgca ggtcccagtc gagcatcggc ggcgtcatgc
     3841 ggccgatcga gccgccgcga tagatcaggg cgccgttgtc gccgatgcga aacgtcggat
     3901 ccgccgcttg ctcgacggcc ttcacccagc cgtgcgaata catgtgcgtg tgcacgtcga
     3961 cgactttggc gcgcgattgg gtctgcgctt cgccgcgcgc ggcgaccgcg gcggcgaggg
     4021 cgcccgcgcc cgcgagcagc tctctgcgat tcatcgtcat ggttacctcg gcggcaatgc
     4081 gaagcgctca gtgtgcatca actcgcggtc tacgagctca agaacgcgac cagcgcgtcc
     4141 gtgacggcct gcggctgctc gagcggcgac agatggccac acgccggcac gacgacctgg
     4201 cgagcaccgg ggatggccgc cgccatctcg gccgcgcggt cgggcggcgt cagcacgtcg
     4261 tcggcgccga cgagcacgag cgtcgggcag cgaattgccg cgagccccgg ccgcgagtcg
     4321 acgcgattca tgatcgcggt ttgctggcga acgaacgcct cgggtcccgt gtcgtccgcc
     4381 atcgtgcgca cgaccgcttg cagcgcacga tcgccgtgcc gcgcggccgc gacgaaccgc
     4441 ggaaagagca gctgcgcgat gccgtcgaag cggccgttcc gcgccatctc gatttgccgt
     4501 ttgcgctgct cggtctgctc gggcgcgtcg gggcgcgccg tcgtgtcgag caatgcgagc
     4561 ttcgcgacgc gatccggcgc ttggcgctcg acctcgaacg cgacgtagcc gcccatcgag
     4621 agcccgatca gcgcgaaccg cggcggcgcc tgcgcgagga tccggcgcgc aatgccgccc
     4681 atgctgtcgt cgcgtgtgtg gtccgcgacc gtcacggcgc cgaaacgcca gaccttggga
     4741 atctgctcgg cgtaaagacg cggcgtgcag agcaagcccg ggatcagcac cgtcggcagg
     4801 gagtcgctca tcagcgcggc cgatggcggc gctcgagctc ggcgacgacg tcggcgagcg
     4861 tcaggtcgcg cagcgcgagc agcagcaaca ggtgatagac gaggtcggcc gcctcggccg
     4921 tgacgcgcgc ctggtcttgc gccgcggcgg cgagcgcgag ctcgacgcct tcctcgccga
     4981 gcttttgcgc gaccttgagc atgccctggg aggccagctt cgccgtatag ctgccctcgg
     5041 gcgcgtcggc gagccgctgg cggatgacct gctgcaattt ggccaagaat tccgtatcgc
     5101 tcacggggcc tcctcgtcaa cgggccgaat cgcgatgccg gccgcggcga gatagcgctt
     5161 cagctcgccg atctcgatcg tcgccgtgtg gaacacgctc gcggcgagcg cgccgtcgac
     5221 gcgcgcgcgg ttgaacactt ccgcgaagtg ctcgcgcgct ccggcgccgc ccgaggcgat
     5281 cagcggcacc tggcagatcg cgcgaacggc ggcgagctgc ggcacgtcgt agccttggcg
     5341 cacgccgtcc tggttcatgc agttcagcac gatctcgccg gcgccgcgat cttgcacttc
     5401 gcgcacccag tctagcgtgc ggcgctctgc ggagcgcgag cgcgtggggt cgcccgtgta
     5461 ttgatagacg tggtagtcgc cgtcgcgcgt cagcgtgtcg acgccgatca cgacgcattg
     5521 cgagccgaag cggcgcacga gctcgtcgat caggtccggc cgctcgaggg cgggggagtt
     5581 cacggagatc ttgtcggcgc cgagattcaa cacggtctcc gcgtcggcga cgctgcggat
     5641 gccgcccgcg acgcagaacg ggatgtcgag cacggccgcg atgcgattga cccaggtgcg
     5701 gtcgacggtg cgccggtccg ggctcgccgt gatgtcgtag aacacgagct cgtcggcgcc
     5761 ttcgtcgcga tagcgcgccg cgagcgtcat gatgtcgccg acgaccttgt gatcgcggaa
     5821 ctgcacgcct ttcacgacga cgccgtcgcg gacgtcgaga cagggaatta tgcgccgcgt
     5881 aagaatggtc ggatctcctc gggtttgagc ctgccttcga gcagcgcttt gccggacacg
     5941 gtcgccgcga cgccggtcgc cgcgagcgcc gccagatcgc cggcgtcgcg cactccgccc
     6001 gaggcttgga actcgatgcc gggccagcgc ctcacgcact cgcgatagag ctcgacgttg
//
LOCUS       DASSNN010000740         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1638505, whole genome shotgun sequence.
ACCESSION   DASSNN010000740 DASSNN010000000
VERSION     DASSNN010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638505"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VFL84_13925"
     CDS             complement(<1..813)
                     /locus_tag="VFL84_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET7609774.1"
                     /translation="MLQCRVLEKRAYDLFMQNLIKGTSHLALGQEAIAAGFGIAMRPD
                     DYTFCTYRGHNHTLVRGVGMTEILGELMGNSSGLMRGKGGSMHLTSVKHHMMGSYAII
                     GAHMTIANGAAWSAQYRGTEQVAVCFFGDGTTNIGAFHEALNFAVIWKLPVVFVCENN
                     LYMEYTNIHDVTAVEHPAADRAASYGLEPVLIDGNDVDVVYRTAQRALDLARKGGGPS
                     LIEAETYRHGGHSRADPGKYRPDDEVQAWLAKDPIPRYRAALTARGATDAQLD"
     gene            1087..2109
                     /locus_tag="VFL84_13930"
     CDS             1087..2109
                     /locus_tag="VFL84_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HET7609775.1"
                     /translation="MVAVHTRIRVILLGFALAALAGCGAAEPGAATSSSEPYVIKFPH
                     VVAPATPKGIMAQRFKELAEERFPGRVRVEVYPSAQLMEDTESIEALAFGEVQMIAVS
                     LSLFDRLTPKFQVFDLPFLFPNLAAVERFQASPAGQALKTTLTDQGILGLQFWHNGMK
                     QLTGPRPLIEPDDARGLKFRVMESDILQAEIEAIGGSPQKMAFGEVFQALQTGTVDAQ
                     ENTWSNIYSSKFYEVQPYMTESNHGYIGYYVAVNGKFWSSLPAELRAGLETTLAEVTE
                     WGNARSEEINQQDKQRIIDSGRTQLTVLTPQQLTKWQSAMRPVWDEFRDAIGADLVNA
                     ALAAAQ"
     gene            2135..2626
                     /locus_tag="VFL84_13935"
     CDS             2135..2626
                     /locus_tag="VFL84_13935"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HET7609776.1"
                     /translation="MQRLSKAADRLEETFMIVALVFMTVLTVVQVVLRYGFGSGLVWS
                     LEATTYTFAWLVLIGMSYGVRTEAHIAVDLVTSKLTPAGATVFAAIALAASLAYCALM
                     IYGSALFVDRLMALGTNARDIPLPRWVLTGIMPIAFLLLALRLVQAALPKLRPRGTAA
                     RSR"
     gene            2623..>3282
                     /locus_tag="VFL84_13940"
     CDS             2623..>3282
                     /locus_tag="VFL84_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET7609777.1"
                     /translation="MTAAFLVVALLALLLLRVPIAISLGLSAVLTMLLFAEQSLVALA
                     QRFFSALQVYPLLAIPFFILAGALMTTGGVAKRMIAFANALVGHFRGGLAMAALLACA
                     LFAAVSGSSPATVVAVGSIMIAGMARSGYTREFAAGLICNAGTLGILIPPSIVMVVYG
                     AVTETSIGDLFLAGIVPGLMLTGMLMIAVAFRARRLDLPRQPRADWRTVLTTGREAIW
                     GL"
BASE COUNT          478 a         1075 c         1111 g          618 t
ORIGIN      
        1 gtcgagctgg gcgtccgtcg cgccgcgcgc cgtcaacgcc gcccggtacc gcgggatcgg
       61 gtccttcgcg agccaggcct gcacctcgtc gtcgggccgg tacttgccgg ggtcggcgcg
      121 cgagtggccg ccgtgacgat acgtttcggc ttcgatcaag ctcgggccgc cgcctttgcg
      181 cgcgaggtcg agcgcgcgct gcgcggtgcg atatacgacg tcgacgtcgt tgccgtcgat
      241 gagcacgggc tcgagcccgt acgaggccgc acggtccgcg gccggatgct cgacggccgt
      301 gacgtcgtga atgttcgtgt actccatgta caggttgttc tcgcagacga acacgacggg
      361 gagcttccaa atcacggcga agttcagcgc ctcgtggaac gcgccgatgt tcgtcgtgcc
      421 gtcgccgaag aaacacacgg cgacctgctc ggtgccgcga tactgcgcgg accacgcggc
      481 gccgttcgcg atcgtcatgt gcgcgccgat gatcgcgtac gagcccatca tgtggtgctt
      541 gacgctcgtc aagtgcatgg agccgccctt gccgcgcatg aggccggacg agttgcccat
      601 gagctcgccc aagatctccg tcatgccgac gccgcgcacg agcgtgtggt tgtggccgcg
      661 ataggtgcag aacgtgtagt cgtcgggccg catcgcgatg ccgaacccgg cggcgatggc
      721 ttcctgaccg agtgccagat ggctcgtgcc cttgatcagg ttctgcatga acaagtcgta
      781 agcgcgcttc tcgagcacgc ggcactggag catcgttcta tagagcttca ggcgcgtctc
      841 ggtgctgatc gagagttgcg gattacgggc ggtgaagccc gccacggtgt gtgcttccac
      901 ttctatatgc cttgttgatg agaggggcgt tctaacgatc cggattcggc ggccgccctc
      961 gcggtcgtgc cgcaaccggc cggaatccga cggttttcct tggctagaag gccgcttagc
     1021 ctagcaagat gctctcggct cggccagact gttcatcacg taatccgctc gcgataaggg
     1081 ggaatcatgg tggcggtgca cacgcgcatt cgcgtgattc tgctgggctt cgcgctcgcc
     1141 gcgctggcgg ggtgcggtgc cgcggaaccc ggcgccgcga cgtcgagcag cgagccgtac
     1201 gtcatcaagt ttccgcacgt cgtcgcgccc gcgacgccga aaggcatcat ggcccagcgc
     1261 ttcaaggagc tcgccgaaga gcgtttcccg ggccgcgtgc gcgtcgaggt ctatccctcc
     1321 gcgcagctca tggaggacac ggagtcgatc gaggcgctcg cgttcggcga ggtgcagatg
     1381 atcgcggtct cgttgtcgtt gttcgatcgg ctcacgccta aattccaggt gttcgacctg
     1441 ccgttcttgt tcccgaatct cgcggcggtc gagcgctttc aggcgagccc ggccggtcag
     1501 gcgctgaaga cgacgctgac ggatcaagga atcttgggcc ttcagttctg gcacaacggc
     1561 atgaaacagc tcacgggccc gcggccgctg atcgagcccg atgacgcgcg cggcctgaaa
     1621 tttcgcgtca tggaatccga catcctgcaa gccgagatcg aagcgatcgg cggcagcccg
     1681 cagaagatgg cgttcggcga ggtctttcag gcccttcaga cgggcaccgt cgacgcgcag
     1741 gagaacacct ggtcgaacat ctactcgtcg aagttctacg aggtgcagcc gtacatgacc
     1801 gagagcaatc acggctacat cggctattac gtcgcggtca acggaaagtt ctggagctcg
     1861 ctgcccgccg agctgcgggc cgggctcgag acgacgctgg ccgaggtcac ggagtggggc
     1921 aacgcgcgct cggaagagat caaccagcaa gacaagcagc gcatcatcga ctcgggccgc
     1981 acgcagctca cggtgctgac gccgcagcag ctcacgaagt ggcagagcgc gatgcgcccc
     2041 gtctgggacg agttccgcga cgcgatcggc gccgatctcg tcaacgccgc gctcgcggcc
     2101 gcgcagtaaa caccgtctct cgatgccatc gctcgtgcag cgcctgtcga aggctgcgga
     2161 ccggctcgaa gagacgttca tgatcgtcgc gctcgtcttc atgacggtgt tgaccgtcgt
     2221 gcaggtcgtg ctgcgctacg gcttcgggtc gggtctggtc tggtcgctcg aggcgacgac
     2281 ctacacgttc gcgtggctcg tgctgatcgg catgtcgtac ggcgtgcgga ccgaagcgca
     2341 catcgccgtc gatctcgtga cgagcaagct cacgccggcc ggcgccacgg tgtttgccgc
     2401 catcgcgctc gcggcgagcc tcgcgtactg cgcgctgatg atctacggca gcgcgctgtt
     2461 cgtcgatcgc ttgatggcgc tcggcacgaa cgcgcgcgac attccgctgc cgcgctgggt
     2521 gctgaccggc atcatgccga ttgcgttcct gctcctcgct cttcggctcg tgcaggccgc
     2581 gcttcccaag ctgcggccgc gcggcacggc ggctcgttcg cgatgacggc cgcgttcctc
     2641 gtcgtcgcgc tgctcgcgct gctgctcctg cgcgtgccga tcgcgatctc gctcggcttg
     2701 tccgcggtgc tgacgatgct gctgttcgcc gagcagtcgc tcgtcgcgct cgcgcagcgg
     2761 ttcttcagcg cgctgcaagt ctatccgctg ctcgcgatcc cgttcttcat cttggccggc
     2821 gccctgatga cgacgggcgg cgtcgcgaag cgcatgatcg cgttcgcgaa cgcgctcgtc
     2881 ggccattttc gcggcgggct cgcgatggcg gcgctgctcg cgtgcgcgct gttcgcggcg
     2941 gtctcgggtt cgtcgccggc gaccgtcgtc gccgtcggct cgatcatgat cgcgggcatg
     3001 gcgcgctcgg gctacacgcg cgagttcgcg gccggcttga tctgcaacgc cggcacgctc
     3061 ggcatcctga tcccgccgtc gatcgtcatg gtcgtttacg gcgccgtcac cgagacctcg
     3121 atcggcgact tgtttctggc gggcatcgtg ccggggctca tgctgaccgg gatgctgatg
     3181 atcgccgtcg cgttccgcgc gcgccggctc gacttgccgc gacagccgcg cgccgattgg
     3241 cgcacggtgc tcacgactgg ccgcgaggcg atctgggggc tg
//
LOCUS       DASSNN010000741         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1714932, whole genome shotgun sequence.
ACCESSION   DASSNN010000741 DASSNN010000000
VERSION     DASSNN010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714932"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VFL84_13945"
     CDS             complement(<1..983)
                     /locus_tag="VFL84_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609778.1"
                     /translation="MFPIGRRCAPPERRLPARRLARRAARIALPVLASLSLGVAALAG
                     AQQNLGFETLDGGALSAWSVAGGSAEIASGVDAAEGEQSLEIQRAEPGVTRVTQRIPA
                     ARLRVGGDAPRLRLTGLTRQHDSRISAALWLRIDGKRGPLFLDSGGYAREPGESPAAS
                     PQSAGGGKEWRRLEVELPFPRDVDEVAFGVIVRGQGTAWFDALELRVVAVDSERAASP
                     AATRYLGEALALMREHSLRRAEIDWPALRAQALEHARGAVTDADAHLAVRFALRELGD
                     RHSYLQSAAATRALAATAVANARTGSPLTPPRARPLGANLAYVSVPGFAGGT"
     gene            complement(949..>2491)
                     /gene="mdoH"
                     /locus_tag="VFL84_13950"
     CDS             complement(949..>2491)
                     /gene="mdoH"
                     /locus_tag="VFL84_13950"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HET7609779.1"
                     /translation="RKVGNLHDFITRWGGRYDFMVVLDADSILAPETLVTLVREMAAD
                     PNLGLLQTVPRLAGGRTLYARLQQFAGRVYGPIVARGIASWQGDDGNYWGHNAIVRVR
                     AFAAAAGLPVLPGKKPFGGAILSHDFVEAALLRRAGWSVRMLPTLGGSWEDSPPSLLD
                     SAARDRRWAQGNIQHLAVILSQGFTWSNRVHMGIGVMSYLASPLWFALLIVGLGTAGH
                     IATVQFEYFTDELSLFPRWPLFDSERMIRLFIIAMATLLTPKILGVLRSFVDGELLRK
                     VNPLRVALGAVAETILSALYAPIMMMMQVRQVSEIFIGQDSGWTTQSRKHARLPWGML
                     FRRHWLQMLSGLVVSVVLLYVSPPLFLWMSPALVGLVLALPLSAASGSTLLAKVSRFL
                     GLLTVPEEVNVPRVIAARDEAEARLEELLDGVTIERLLSDELARQRHFAAVQPRPPAT
                     RGHPDVMFITARAKLGDAHTAAEALSWLSPPERLAVLGDRDLFHVLVRLVNPDVPDRP
                     ALRSA"
BASE COUNT          415 a          887 c          872 g          317 t
ORIGIN      
        1 gtgccgcccg cgaatcccgg cacgctgacg taggcgaggt tcgcgccgag cggccgcgcg
       61 cgcggcggcg tcaacggcga ccccgtgcgc gcgttcgcga cggccgtcgc ggcgagcgcg
      121 cgcgtcgcgg ccgcgctttg cagatagctg tgccggtcgc cgagctcgcg cagcgcgaag
      181 cgcacggcga ggtgagcatc ggcgtcggtc acggcgccgc gcgcgtgctc gagcgcctgc
      241 gcgcgcaacg ccggccagtc gatctcggcg cgccgcaacg aatgctcgcg catcagcgcg
      301 agcgcctcgc cgaggtaacg tgtcgccgcc ggcgaagccg cacgctccga atcgacggcg
      361 acgacgcgca gctcgagcgc gtcgaaccac gccgtgcctt ggcctcggac gatgacgccg
      421 aacgcgacct cgtcgacgtc gcgcgggaac ggcagctcga cctcgagccg gcgccactcc
      481 ttgccgccgc cggcgctttg cggcgacgcg gcgggcgact cgccaggctc gcgcgcatag
      541 ccgccgctgt cgaggaacaa cgggccgcgc ttgccgtcga tgcgcagcca gagcgcggcg
      601 gagatacgcg agtcgtgttg ccgcgtgagg ccggtcagcc tcaagcgcgg cgcatcgccg
      661 cccacgcgca gccgcgccgc ggggatgcgc tgagtgacgc gcgtgacgcc gggctccgct
      721 cgctgaattt cgaggctctg ctcgccctcg gccgcgtcga cgccgctcgc aatctcggcg
      781 ctgccgcctg cgacgctcca cgcggacagc gcgcctccgt cgagcgtctc gaagccgagg
      841 ttctgctgcg cgccggcgag ggccgcaaca ccgagcgaca gcgaggcgag gacgggaagc
      901 gcaattcgcg ccgcgcgccg tgcgaggcgc cgcgccggaa ggcgccgctc aggcggagcg
      961 cagcgccggc cgatcgggaa catcggggtt cacgagccgc acgagcacgt ggaacaggtc
     1021 gcgatcgccg agcactgcca atctttccgg cggcgaaagc cacgacaaag cctcggccgc
     1081 cgtgtgcgcg tcgccgagct tcgcgcgcgc ggtgatgaac atgacgtcgg gatggccgcg
     1141 agtcgccggc ggccgcggct gcacggcggc gaaatgccgt tgccgcgcga gctcgtcgct
     1201 caagagcctc tcgatcgtga cgccgtcgag cagctcttcg agccgcgctt ccgcttcgtc
     1261 gcgcgccgcg atgacgcgcg gaacgttgac ttcctctggc accgtcagaa gacccaggaa
     1321 gcgcgagact ttcgcgagca acgtgctgcc gctcgcggcg gacaacggca gcgcgagcac
     1381 gagacccacg agagccggag acatccacaa gaacaacggc ggcgaaacgt agaggagcac
     1441 gaccgagacg accagacccg aaagcatttg cagccagtgg cggcggaaca gcatccccca
     1501 cggcaggcgc gcgtgcttgc ggctctgcgt cgtccagccg gagtcctggc cgatgaagat
     1561 ctcggacacc tggcgcactt gcatcatcat catgatcggc gcgtacagcg ccgagagtat
     1621 cgtctcggcg acggcaccga gcgcgacacg caacggattg accttgcgca gcagctcgcc
     1681 gtcgacgaac gagcgaagca cgcccaagat cttcggcgtc aacagcgtcg ccatcgcgat
     1741 gatgaagagc cggatcatgc gttcgctgtc gaacaatggc cagcgcggga acaacgacag
     1801 ctcgtccgtg aagtattcga actggaccgt cgcgatgtga cccgcggtgc cgaggccgac
     1861 gatgagcagc gcgaaccaca gcggtgaggc gagatagctc atcacgccga tccccatgtg
     1921 gacgcgattc gaccacgtga acccttggct taagatcacg gcgaggtgct ggatgttgcc
     1981 ctgcgcccag cgccggtcgc gcgcggccga gtcgagcagg gacggcgggc tgtcctccca
     2041 gctgccgccg agcgtcggca gcatgcgcac cgaccacccg gcgcggcgca gcagcgcggc
     2101 ttcgacgaaa tcgtgggaga ggatcgcgcc gccgaacggc ttcttgcccg gcaacaccgg
     2161 caagccggcc gccgcggcga acgcgcgcac gcgcacgatc gcgttgtgtc cccagtaatt
     2221 cccgtcgtcg ccttgccagc tcgcgatgcc gcgcgcgacg atcggcccgt aaacccgccc
     2281 cgcgaactgc tgcagccgcg cgtacaacgt tcggccgccc gcgagccgag gcacggtttg
     2341 caggagcccg aggttcggat ccgccgccat ctcgcgcacg agcgtcacga gcgtttcggg
     2401 agccaggatg ctgtcggcgt cgagcacgac catgaagtcg tagcggccgc cccagcgcgt
     2461 gatgaaatcg tgcaggttgc cgaccttgcg g
//
LOCUS       DASSNN010000742         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1512318, whole genome shotgun sequence.
ACCESSION   DASSNN010000742 DASSNN010000000
VERSION     DASSNN010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512318"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..945)
                     /locus_tag="VFL84_13955"
     CDS             complement(70..945)
                     /locus_tag="VFL84_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609780.1"
                     /translation="MSEWIDFAVYVTLIVLIFVLLPRHGRQFTLPMIADRNPDWPARN
                     RDVVARLERSRWFLNACYVWAVVSIVVLLGVQLGLIAPPLDSNAPKWEILKDVNSTFL
                     ILGLLCWGVCWLLWVRWLATHVPPAETRRATLKPRVIGDYLSVRWRVAVDALTVLHLS
                     TWVVIGALGLAGGAKFWGSFAFFMVMTLAFRIFGYLAPRRRPGYLDRLFGEAYRRSEI
                     GLAYFMRLWPAASGAIVLAELTTGADLDRAAQLLVVSFVCVLALMILRLRPVAPGTGP
                     VSGHGPFASERRSPA"
     gene            complement(942..1337)
                     /locus_tag="VFL84_13960"
     CDS             complement(942..1337)
                     /locus_tag="VFL84_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7609781.1"
                     /translation="MEITVHTASPEPVYEQIVRQIQDRVASGELKPGASLPTVRQLAG
                     DLQLNRNTVARAYKQLEDRGVILTAGRKGTFVRDNASREVERIKSNRAERSVRHLVAA
                     LLGEGLSRAEIEALFNDALRVAQPKETTP"
     gene            complement(1389..1916)
                     /locus_tag="VFL84_13965"
     CDS             complement(1389..1916)
                     /locus_tag="VFL84_13965"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET7609782.1"
                     /translation="MKSVISIELDGTTFTLDERAYAALRAYLDRAGERLGAHPDRAEV
                     VAGLERSIAAQLRSGAQAVATIDEAAMLAALKAVGRVDGPALHDSASSDNVGYREPRT
                     RRLYRLREGHWIVGVCTGLAAYARIDPVLIRLIFILAGVFSGGVAILVYVILAFVMPI
                     ALTDDEISEAHGGMR"
     gene            complement(1934..>2395)
                     /locus_tag="VFL84_13970"
     CDS             complement(1934..>2395)
                     /locus_tag="VFL84_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609783.1"
                     /translation="GGTFRCQEPRDVMLIFDITEEEHPVAISTFQVPEEPGDFCHRGG
                     RFGPHSFADAYHPSFDKKLVVLAYFNAGVRVVDIRNPFEPREVARFIPEVTENTTESC
                     IEIDGARQCDRAIQTNNVNLDDRGYIYALDRASTGLHVFELTGEARAIAGL"
BASE COUNT          443 a          794 c          781 g          377 t
ORIGIN      
        1 gatggggcgc ggcatcgcgc ggcgagccgt cgcgtcgctg atgacgccac gtgaccaagc
       61 gtctgccggt tatgccggcg accggcgctc ggacgcgaac ggtccgtgac ccgataccgg
      121 gccggtgccc ggcgcgacgg gtcgcaagcg caggatcatg agcgccagca cgcacacgaa
      181 cgaaacgacc agcaactgcg ccgcgcggtc gagatcggcg cccgtcgtca gctcggccaa
      241 gacgattgcg ccgctggccg ccggccacag ccgcatgaag tacgcaagcc cgatctcgct
      301 gcggcgatag gcttctccga acaggcggtc gagatagcca ggccggcgcc gcggagcgag
      361 gtaaccgaag atcctgaacg ccagggtcat gaccatgaag aacgcgaagc ttccccagaa
      421 cttcgcaccg cccgccaacc cgagcgcgcc gatcacgacc caagtcgaaa gatgcagcac
      481 cgtcagcgcg tcgacggcga cgcgccaacg aacactgagg tagtcgccga tgacgcgcgg
      541 cttcagcgtg gcacggcgcg tttcggcggg cggcacgtgc gtggctagcc aacgaaccca
      601 aagcagccag cacacgcccc agcagagcaa gccgagaatc aagaaggtgc tgttcacgtc
      661 cttcaagatc tcccacttcg gtgcgttcga gtcgagcggt ggagcaatga ggccgagctg
      721 cacgccgagc aacacgacaa tgctgaccac ggcccataca tagcacgcgt tcaaaaacca
      781 gcggctgcgc tcgaggcgcg cgacgacgtc gcgattacgc gcgggccagt ccggatttcg
      841 gtccgcgatc attggcagcg tgaactgcct gccgtgacgc ggcagcagca cgaagatcaa
      901 cacgatcaac gtgacgtaaa cggcaaagtc gatccattcg ctcatggtgt cgtttccttc
      961 ggttgcgcga cgcgcagcgc gtcgttgaac aaagcttcga tctcggcgcg gctcaagccc
     1021 tcgccgagca gcgctgcgac gaggtgccgc acgctgcgct cggcgcgatt cgacttgatg
     1081 cgctcgacct cgcgcgaggc gttgtcgcgc acgaacgtgc ccttgcggcc cgcggtcagg
     1141 atcacgccgc ggtcctcgag ctgcttgtag gcgcgtgcga ccgtattgcg gttcagctgc
     1201 aggtccccgg cgagctggcg caccgtgggc agcgacgccc cgggcttgag ctcgccggat
     1261 gcaacgcggt cctggatctg ccggacgatt tgctcgtaga ccggctcggg cgaggccgtg
     1321 tgtacagtga tttccatttg tcctagtttc aactaggaca aatggaaagt caagccgtcg
     1381 acgcagcttc agcgcatgcc gccgtgggcc tcgctgatct cgtcgtccgt cagcgcgatc
     1441 ggcatcacga acgcgaggat cacgtagacc agtatcgcta caccgccgga aaacactccg
     1501 gcgaggatga agatcaacct gatcaagacg gggtcgattc tcgcgtacgc ggcgagcccc
     1561 gtgcagacgc ccacaatcca gtggccctcg cgtaagcgat agagcctgcg cgttcgcggc
     1621 tcgcggtagc cgacgttgtc actcgacgcg gaatcgtgga gcgccgggcc gtcgacgcgg
     1681 ccgacagcct tcagtgccgc aagcatcgcg gcctcgtcga tcgtggcgac agcctgtgcg
     1741 cctgagcgca gctgcgccgc gatcgagcgc tcgagccccg cgaccacctc ggcacgatcc
     1801 ggatgcgcgc cgagccgctc gccggcgcga tcgagatagg cgcgcaacgc cgcgtaggcg
     1861 cgctcgtcga gcgtgaacgt cgtgccgtcg agctcgatcg agattaccga tttcatcgtt
     1921 tgcagctctc cgtctagagg cccgcgatcg cgcgcgcctc gcccgtcagc tcgaagacgt
     1981 gcaaccccgt cgacgcgcga tcgagcgcgt agatgtagcc gcggtcgtcg aggttcacgt
     2041 tgttcgtctg gatcgcacgg tcgcactgcc gcgcgccgtc gatctcgatg cacgactcgg
     2101 tcgtgttctc ggtgacctcg gggatgaagc gcgcgacctc tctcggctcg aacggattgc
     2161 gaatgtcgac gacgcgcacg ccggcgttga agtacgcgag cacgacgagc ttcttgtcga
     2221 agctcgggtg atacgcgtcg gcgaacgagt gcggcccgaa gcgtccgccg cgatgacaga
     2281 aatcgccggg ctcctcgggc acctgaaacg tcgagatcgc tacgggatgc tcttcttcgg
     2341 tgatgtcgaa gatcagcatg acgtcgcgcg gctcttggca acggaacgtg cctcc
//
LOCUS       DASSNN010000743         3247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1332483, whole genome shotgun sequence.
ACCESSION   DASSNN010000743 DASSNN010000000
VERSION     DASSNN010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332483"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFL84_13975"
     CDS             <1..583
                     /locus_tag="VFL84_13975"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HET7609784.1"
                     /translation="ASLPAFTIAVAAPPNRPPTISGAPATSVLQGTLYSFQPAASDPD
                     GNALTFSISSKPSWATFDSATGRLQGTPAPADVGTTANVVISVSDGSASASLPAFSVA
                     VQAVATGSATLTWLPPTTNTDGTPLTNLAGYRIYWGTSPGNYSSSATVMSPGITSYVV
                     ESLTPNTYYFAVKALNSAGAESVFSNVASKTIR"
     gene            complement(627..1148)
                     /locus_tag="VFL84_13980"
     CDS             complement(627..1148)
                     /locus_tag="VFL84_13980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7609785.1"
                     /translation="MNIVACDYEHHAAPILAILNRAIVSSTALFDYKPRAPETMVEWF
                     RAKTARQFPVLGAVGDDGALLGFATYGTFRAWPAYKYTIEHSVYVHEQHRRGGVARAL
                     MTRLIAAAEEQSYHVMVGGIDATNAPSIALHEQLGFTRCGVIHHAGFKFGRWLDLVFY
                     ELQLRTPARPVDG"
     gene            complement(1167..3044)
                     /locus_tag="VFL84_13985"
     CDS             complement(1167..3044)
                     /locus_tag="VFL84_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7609786.1"
                     /translation="MKLVDFDIPATASPSVGAPPEGLVAFYWHFIRQTKGWYAAMFAA
                     SLTVALLDTVIPIFIGRLVALMGAPDRAAALAAALPMLLGMVALVLVARPLAIFMDIS
                     IRHNALVPGATSLIRWQSHWHVVRQNWPFFQNDFAGRIANRVMQTASSLRESAMSSIR
                     AVWYIGVYGVTAFGLMMAADWRLTVPIATWAVGYVFVLKHFVPRLRELSRVSSEGKSL
                     VMGRVVDSYTNILTVKLFGRMRDEDAFVREAMDGHYGAMRRHLRMITQFQISLTVLNA
                     LLLVGTGAIGITLWAQGSIDAGQVATALPLAWQTVNAAGWVSWEVTSIFENIGTVQEG
                     MQSIAVPLSGVDRPGARPLDVRRGEIKFENLTFAYGRSDSAPVVEKLNLTIRAGERVG
                     LVGRSGAGKSTLVNLLLRLHETESGRIAIDGQDIANVTQESLRAAIGMVTQDTSLLHR
                     SIAANIRYGKPSASDAELVAAARKAQAHEFIVGLRDWHGRTGYAAHVGERGVKLSGGQ
                     RQRVALARVILKDAPILVLDEATSALDSEVELAIQEQLLDLMQGKTVIAIAHRLSTIA
                     RMDRLVVLDAGRVVEEGTHDELLRRRGHYEKLWRHQSGGFIANELDESDEPPTLRPAV
                     G"
BASE COUNT          540 a         1119 c         1091 g          497 t
ORIGIN      
        1 cgcgtcgttg cccgcgttca cgatcgcggt tgcggcgccg ccgaaccggc cgccgacgat
       61 ctccggcgct ccggccacgt cggtgctgca agggacgctc tattcatttc aaccggcggc
      121 ttccgatccc gacgggaacg cgctgacgtt cagcatctcg agcaagccgt cgtgggcgac
      181 gttcgactcg gcgacgggcc gactgcaggg cacgcccgcg ccggccgacg tcggcacgac
      241 cgcgaacgtc gtcatcagcg tcagcgacgg cagcgcgagc gcgtcgttgc cggccttcag
      301 cgtcgccgtg caagccgtcg cgacgggctc ggccacgttg acctggctgc cgccgacgac
      361 gaacacggac ggcacgccgc tgacgaatct cgccggttac aggatttact ggggcacgtc
      421 gcccggcaac tactcgagct ccgcgacggt catgagcccc ggcatcacga gctacgtagt
      481 cgagagcctc acgccgaaca cgtactactt cgccgtcaag gcgctcaaca gcgccggcgc
      541 cgagagcgtg ttctcgaacg tggcgagcaa gacgatccgg tgagcggcgg cttggcgtga
      601 gcgaggcgcg cgccgcccac cctgcctcag ccgtcgacgg gccgagcagg cgtgcgcagt
      661 tgcagctcgt agaagacgag gtcgagccag cgcccgaact tgaagccggc gtgatggatc
      721 acgccgcagc gagtgaagcc gagctgctcg tgcagcgcga tgctcggcgc gttcgtcgcg
      781 tcgatgccgc cgaccatgac gtgatagctc tgctcctcgg cggcggcgat caagcgcgtc
      841 atgagcgcgc gcgcgacgcc gccgcggcgg tgctgctcgt gcacgtagac cgagtgctcg
      901 atcgtgtact tgtacgcggg ccaagcacgg aacgttccgt acgtcgcgaa gccgagcagc
      961 gcaccgtcgt cgccgaccgc gccgagcacg gggaactggc gcgcggtctt cgcgcggaac
     1021 cactcgacca tcgtttcggg ggcgcgcggc ttgtagtcga acaacgccgt cgagctcacg
     1081 atcgcacggt tcaagatcgc gaggatcggc gcagcgtgat gctcgtaatc gcaagcgacg
     1141 atgttcacgg cgctttcccc gtacggctag cctacggccg gtcgtaatgt cggaggctcg
     1201 tcgctctcgt cgagctcgtt cgcgatgaag ccgcccgatt ggtggcgcca gagcttttcg
     1261 tagtgtccgc ggcggcgcag cagctcgtcg tgcgttccct cttcgacgac tcggccggcg
     1321 tcgagcacga cgagccggtc catgcgcgca atcgtcgaaa gccgatgcgc gattgcgatg
     1381 accgtcttgc cttgcatcaa gtccaacagc tgctcctgga tcgcgagctc gacctcgctg
     1441 tcgagcgccg acgtcgcctc gtcgagcacg agtataggcg cgtccttcaa gatcacgcgc
     1501 gcgagcgcga cgcgctgccg ttgcccgccc gatagcttga cgccgcgctc gccgacgtgc
     1561 gccgcataac ccgtccggcc atgccaatcg cgcagcccga cgatgaactc gtgcgcctgc
     1621 gccttgcgcg ccgccgcgac gagctcggcg tcgctcgcgg acggcttgcc gtaacggatg
     1681 ttcgcggcaa tcgaacggtg cagcagcgac gtgtcctgcg tcaccatgcc gatcgccgct
     1741 cgcaggctct cctgcgtgac gttcgcgatg tcctggccgt cgatcgcgat gcggcccgac
     1801 tcggtctcgt gcagccgcag cagcaagttc acgagcgtcg acttgccggc tccggagcgg
     1861 ccgacgagac ccacgcgctc gccggcgcga atcgtcaggt tcaacttctc gacgacgggc
     1921 gccgaatcgg agcggccata ggcgaacgtc aggttctcga acttgatctc gccgcgcctg
     1981 acgtcgagcg gccgcgcgcc ggggcgatcg acgccgctca agggcaccgc gatcgactgc
     2041 attccttcct gcacggtgcc gatgttctcg aagatcgacg tgacctccca gctgacccag
     2101 cccgccgcat tgacggtctg ccacgcgagc ggcagcgccg tcgcgacctg gcccgcatcg
     2161 atcgagccct gcgcccacag cgtgataccg atggcccccg tgccgacgag cagcagcgcg
     2221 ttcaacaccg tcagcgagat ctggaactgc gtgatcatgc gcaaatgccg acgcatcgcg
     2281 ccgtagtggc cgtccatcgc ttcgcggacg aacgcgtcct cgtcgcgcat gcggccgaac
     2341 agcttgaccg tcaggatgtt cgtgtagctg tcgacgacgc ggcccatgac caacgacttt
     2401 ccctcggagc tgacgcgcga cagctcgcgc agccgcggga cgaaatgctt gaggacaaag
     2461 acgtagccga cggcccacgt cgcgatcggc accgtgagcc gccagtcggc cgccatcatg
     2521 aggccgaagg ccgtgacgcc gtagacgccg atgtaccaga cggcgcggat gctcgacatc
     2581 gcgctttcgc gcagcgagct cgccgtctgc atgacgcggt tcgcgatgcg gcccgcgaag
     2641 tcgttctgga agaacggcca gttctggcgc acgacgtgcc agtggctttg ccagcggatc
     2701 aggctcgtcg cgccgggaac gagcgcgttg tgccggatcg agatgtccat gaatatcgcg
     2761 agcggccgtg cgacgagcac gagcgcgacc atcccgagca gcatcggcag cgcggccgcg
     2821 agagccgcgg cgcggtcggg cgcgcccatc aacgcgacga gccggccgat gaagatcggg
     2881 atgacggtgt cgagcagcgc gacggtcagg ctcgccgcga acattgccgc gtaccagccc
     2941 ttggtctggc ggatgaagtg ccagtagaac gcgacgaggc cctcgggcgg cgcgccgacc
     3001 gacgggctcg cggtcgcggg aatgtcgaag tcgacgagct tcacggaaga atgtccgcgc
     3061 ggctcacgcg ctgcctccga ctcaagggaa cgccgacggg ttgccggcgg ccgcacgata
     3121 acagattcgc gccgcacgca aattggcatc tcgagaaagc gcgtcaagcc gtcgccgaac
     3181 ggcgacctga tcttccggcg gacttggtca tggacgagtg acggatcgcg cgccggggcg
     3241 cggaccg
//
LOCUS       DASSNN010000744         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_867923, whole genome shotgun sequence.
ACCESSION   DASSNN010000744 DASSNN010000000
VERSION     DASSNN010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867923"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2383
                     /gene="ileS"
                     /locus_tag="VFL84_13990"
     CDS             <1..>2383
                     /gene="ileS"
                     /locus_tag="VFL84_13990"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET7609787.1"
                     /translation="KELGIFDKRRIEQEVGIASFTAQCRASVMRHVAEWEAMTERMGF
                     WVDFEDAYFTLHNDYIESVWYLLKKIWDKGLIYQGYKVVPYDPRIGATLSSHEVAQGY
                     REVEDPSVYVRFKVHGETNTYFLVWTTTPWTLPANLLLAVHPDVEYVWVERGKETLIL
                     AKPLVAAIFKDEDVRIVRTARGRELDGMRYERLFDYLPVAGDVCRVRPAEFVTTEDGT
                     GIVHVAPAYGEDDLKLGQQYGLPVLHAVGEDGYFVAAVTPVAGKFFKDADPTIIEILR
                     ERGLLFRATKYLHNYPFGWRTGDPLIYYAKNAWYIRTTEFRQRMVELNHGIKWVPEHI
                     REGRFGNWLENNIDWALSRERFWGTPLPVWTDGDGDYICIGSRAELEKLSGRTLGELD
                     LHRPAIDSVTFVHEGREYRRVAEVIDCWFDSGAMPYAQWHYPFENAAVFEQSFPADFI
                     CEAIDQTRGWFYTLHAIATMVSDRAAYKNVVCLSHIVDQDGKKMSKSLGNIINPYDVF
                     NAVGADALRWHFTARVAPDVQKRVSVEIIADVASSFINTFWNTYAFFVMYARLDGFER
                     GADVPYARRPEIDRWVLSLLNQTVATATAALDDYDALRAGAAIESFVDQLSNWYVRRN
                     RRRFWKAASGADKQAAYLTLYECLETVNRLLAPFVPFISEAVHQNLVRKVSPDAPVSV
                     HMTAWPDVDARRVDERLLEETAVVQRVVGLGRAARNSSKLKVRQPLPRLLIRVPDAAA
                     EAAVRRHEDQILDELNVKRLELIARDATLVTYRIKPNLPVIGKRYGKLIPKIRA"
BASE COUNT          422 a          792 c          784 g          385 t
ORIGIN      
        1 gaaggagctc ggcatcttcg acaagcggcg catcgagcag gaggtcggca tcgcgagctt
       61 cacggcgcag tgccgcgcgt ccgtgatgcg ccatgtcgcc gaatgggaag ccatgacgga
      121 gcgcatgggc ttctgggtcg acttcgagga cgcgtacttc acgctgcaca acgactacat
      181 cgagagcgtc tggtacctgc tcaagaagat ctgggacaaa gggctcatct atcagggcta
      241 caaggtcgtg ccgtacgacc cgcgcatcgg cgcgaccttg tcttcgcacg aagtcgcgca
      301 aggttaccgc gaggtcgagg acccgtccgt ctacgtgcgc ttcaaggtgc acggcgagac
      361 gaacacgtac ttcctcgtgt ggacgacgac gccgtggacg ctgcccgcga acttgctgct
      421 cgccgtgcac cccgacgtcg agtacgtctg ggtcgagcgc ggcaaagaga cgctgattct
      481 cgcgaagccg ctcgtcgccg cgatcttcaa ggacgaggac gtgaggatcg tgcgcacggc
      541 tcgcggccgc gagctcgacg gcatgcgcta cgagcggctg ttcgactacc tgcccgtggc
      601 gggcgatgtc tgccgcgtgc ggcctgcaga gttcgtgacg accgaggacg gcacgggcat
      661 cgtgcacgtc gcgccggcgt acggcgagga cgacttgaag ctcggccagc aatacggcct
      721 gcccgtgctg cacgccgtcg gcgaggacgg ctacttcgtg gccgccgtga cgccggtcgc
      781 cggcaagttc ttcaaggatg ccgacccgac gatcatcgag atcctgcgcg agcgcggcct
      841 gctcttccgc gcgacgaagt atctgcacaa ctatccgttc ggctggcgca ccggcgatcc
      901 gctgatctac tacgcgaaga acgcgtggta catccgtacg acggagttta ggcagcgcat
      961 ggtcgagctc aatcacggca tcaaatgggt gcccgagcac atccgcgaag gccgcttcgg
     1021 caactggctc gagaacaaca tcgactgggc cctgtcgcgc gagcgcttct ggggaacgcc
     1081 gttgccggta tggaccgacg gcgacggcga ctacatctgc atcggctcgc gcgccgagct
     1141 cgagaagctc tcgggcagaa cgctcggcga gctcgacctg caccggcccg cgatcgacag
     1201 cgtgacgttc gtgcacgaag gccgcgagta ccggcgcgtc gccgaggtca tcgattgctg
     1261 gttcgactcg ggcgcaatgc cgtacgcgca gtggcactac ccgttcgaga acgccgcggt
     1321 gttcgagcag agcttcccgg ccgatttcat ctgcgaggcc atcgaccaga ctcgcggctg
     1381 gttctacacg ctgcacgcga tcgcgacgat ggtgtcggac cgggctgcgt acaagaacgt
     1441 ggtctgcctg agccacatcg tcgaccaaga cggcaagaag atgtcgaagt cgctcggcaa
     1501 catcatcaac ccgtacgacg tcttcaacgc cgtgggcgcc gacgcgctgc gctggcactt
     1561 cacggcgcgc gtggcgcccg acgtgcagaa gcgcgtgtcc gtcgagatca tcgccgacgt
     1621 cgcgagcagc ttcatcaata cgttctggaa cacgtacgcg ttcttcgtga tgtacgcgcg
     1681 cctcgacggc ttcgagcgcg gcgccgacgt gccgtacgcc cggcggcccg agatcgatcg
     1741 ctgggtgttg tcgctgctca accaaacggt tgcgacggcg accgcggcgc tcgacgacta
     1801 cgacgccctg cgcgcgggtg ccgcgatcga gagcttcgtc gaccagctca gcaactggta
     1861 cgtgcgccgt aaccgccgcc gcttctggaa agccgcgagc ggcgcggaca agcaggccgc
     1921 gtacttgacg ctctacgagt gtctcgagac cgtgaaccgc ctgctcgcgc cgttcgtgcc
     1981 gttcatcagc gaggccgtgc accagaacct cgtgcggaag gtctcgcccg acgcgccggt
     2041 cagcgtgcac atgacggcct ggcccgacgt cgacgcgcgc cgcgtcgacg aacgtctgct
     2101 cgaggaaacc gccgtcgtgc agcgcgtcgt gggcttgggg cgcgccgcac gcaactcgtc
     2161 caagctcaag gtgcggcaac cgttgccgcg cctcttgatt cgcgtgccgg atgcggcggc
     2221 cgaggcggcc gtgcgccgcc acgaggacca gatactcgac gagctcaacg tcaagcgcct
     2281 cgagctcatc gcgcgcgacg cgacgctcgt gacctatcgc atcaagccga acctgcccgt
     2341 catcggcaag cgctacggca agctgatccc gaagatccgc gcg
//
LOCUS       DASSNN010000745         3589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_867970, whole genome shotgun sequence.
ACCESSION   DASSNN010000745 DASSNN010000000
VERSION     DASSNN010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867970"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..668)
                     /locus_tag="VFL84_13995"
     CDS             complement(18..668)
                     /locus_tag="VFL84_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609788.1"
                     /translation="MKGRMLEVLAIALMLLASHVTAAADSRDSLAGTWSLDDRSSDDP
                     VEEIGGKHGNGLGRQIVKSVNVFGIPVGSLPLPGDDEENKEDPLTPAQIVGASLAYVF
                     EATYRLRITQNASATEIRYGNAPANIYRDGGMFEHDGWTSKVEWRNGALTIEHERPAD
                     GAHISERYWIEPRADELRWTATLKRPKKATVEVERVFYRAPANQDANLPFTARLSP"
     gene            945..>3589
                     /locus_tag="VFL84_14000"
     CDS             945..>3589
                     /locus_tag="VFL84_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7609789.1"
                     /translation="MTAPTLRRVAAPLVVRLTPLACTVAAALGTIAVPALAQDTGLEE
                     ILVTARYREENIQTTPIAITAFSGEELELRSIENVEDIGQAIPNAYFRRNASNFGPNN
                     TIGLRGLNQIDFSYAFEPTVGIYIDDVYHSTITGSDMDLVDLDRVEVLRGPQGTLFGK
                     NSLGGAIRLVSRAPEGDDTGMVSVTVGDYDRLDLKAMGDFALIDEKLFVRLVGVTKEQ
                     EGYGASLDFTCEMIRRGTPQLAGLNDGLGFGGYGPDNSVLLPPLPGQTTPRPDFDTLP
                     DLLPPIAVPVGSAADNAFSLPAQRNIAQDGSCELGKLGGTSSEAGRVMLRFLPSDKLD
                     INLSVDATNSDDDPNVDTQISRINNQTDNDYDIGVVFPSYGIRYTTDNRFVTGDPYTN
                     YATFSDVVNGQNYPRNARMDSGGVSLVLTYDLTDRTSAKLILADRGYDTEWTNDSDRT
                     PFGLTQTHYVQNHDEQQAELQFSGAAGGNDRLGWTAGLFFFESESRAYNTTEFEAFNY
                     TGGLLNFVANDGYTTDNESAFVHVTYDFTERLRVSGGARYTDETKTNTFDHGPALNRS
                     DVPLIFGDSRTDWKLSVDFSLTDSVFLYAQAATGFSSESATPRIFTVGQLMALAGEEL
                     LSEEIGAKLEFLDSRLRVNAAIFTSDYDPRIRQTGGVNQCDAPTELNPVPYRLQGGTC
                     PPGTFFAGSTGLPWFFYDNSPGTLDGYEVELTATPVERLLISFSLGQNEYENTNNDPG
                     PSNFTYIAPGYLFQPEFNSSLGLQYDFRFGNGGTLSPRIDAFYQSERHTGPANARPAA
                     HEVTANTCPTQCIEDYTTFNARLTYQPPNAEWRLAIFGTNVTNEFYWQQYSPEITVNA
                     TTGAITNTAPFGRTGVASAPREWALT"
BASE COUNT          667 a         1168 c         1119 g          635 t
ORIGIN      
        1 gatcccccgt cgccggctca cggcgacagc cgcgcggtga acggtaggtt ggcgtcctga
       61 tttgcgggcg ctcgatagaa cacgcgctcg acctcgacgg ttgccttctt gggtcgcttc
      121 aacgtcgccg tccagcgcag ctcgtcggcg cgaggctcga tccagtagcg ttcgctgatg
      181 tgcgcgccgt ccgccggccg ctcgtgctcg atcgtcagcg cgccgttccg ccattcgact
      241 ttcgacgtcc agccgtcgtg ctcgaacatg ccgccatcgc gatagatgtt ggcaggagcg
      301 ttgccgtagc ggatctcagt agccgaagcg ttctgcgtga ttcgcaaccg ataggtcgct
      361 tcgaagacgt acgcgagcga cgcgccgact atctgcgcgg gcgtgagagg atcttctttg
      421 ttctcctcat cgtcgccagg cagcggcagg ctcccgaccg ggatgccgaa cacattgacg
      481 cttttcacga tttgccgccc gagaccgtta ccgtgcttgc cgccgatctc ttcgaccgga
      541 tcgtcgctcg agcgatcgtc gagcgaccac gtgcctgcaa gcgaatcgcg cgagtcggcc
      601 gccgctgtca cgtgactcgc cagcaacatc agcgcaatgg ccagcacttc gagcattctt
      661 cccttcatcg caagtctcct cgtgcgacgc gcagagccgc atgctagcga agcgtgctcg
      721 gcgcggtcgt aaccaatcgt gaacgtcgcc ggtcctgccc ggctgccgga ataacgagcg
      781 gactcagcgg ctggttgacg tgcccgtcaa ggaagcgcca ctgcgccgaa ccggatggtg
      841 cgtctcgcgc gcgccgccac cgaccgcggc gtactgttgt ggcgtagcgg cggctgcggc
      901 acactcggac gcgctatacg gcattccgtt ttgaaggggg acccatgact gctccgacgc
      961 tgcggcgagt tgccgcgccg cttgtcgttc gcctcacacc gctcgcctgc acagtagccg
     1021 ccgcgctcgg cacgatcgct gttccagcgt tggcccaaga cacgggcctc gaggaaatac
     1081 tcgttacggc gcgctatcgc gaagagaaca tccagacgac gccgattgcg atcaccgcat
     1141 tctcgggcga agagctcgag ctgcgcagca tcgaaaacgt cgaggacatt ggccaagcga
     1201 ttccgaacgc ttatttccgt cgcaacgcga gcaacttcgg accgaacaac acgatcggct
     1261 tgcgtggtct caatcagatc gatttcagct atgcgttcga gcccaccgtc ggcatctaca
     1321 tcgacgacgt gtaccacagc acgatcacgg gctcggacat ggacctcgtc gacctcgatc
     1381 gtgtcgaggt gctgcgcggg ccgcagggca cgttgttcgg caagaacagc ctcggcggcg
     1441 cgattcgcct ggtctcgcgg gcgccggagg gcgacgacac cggaatggtc tccgtcaccg
     1501 tcggcgacta cgaccgcctg gacttgaagg ccatgggcga ctttgcattg atcgacgaga
     1561 agctgttcgt gcggctcgtc ggcgtgacga aggagcagga aggctacggc gcttcgctcg
     1621 acttcacgtg cgagatgatt cgccgcggga ctccgcagct cgccggcttg aatgacggcc
     1681 tcggcttcgg cggatatggt cccgacaaca gcgtcctgct gccgcccctg ccgggccaaa
     1741 cgaccccgcg cccggatttc gacacgctgc cggacctgct gccgccgatc gcggtgccgg
     1801 tcggcagcgc ggccgacaac gcgttctcgc tgccggcgca gagaaacatc gcgcaggatg
     1861 ggtcatgcga gctcggcaag ctcggcggca cgtcgtcgga ggccggtcgc gtaatgctgc
     1921 gattcctgcc gtcggacaag ctcgacatca acctttcggt cgacgcgacg aacagcgacg
     1981 acgaccccaa cgtcgacacg caaatctcgc gcatcaacaa tcagacggac aacgactatg
     2041 acatcggcgt cgtgttcccg agctacggca tccgatacac cacggacaac cgcttcgtaa
     2101 cgggtgaccc gtacacgaat tacgcgacgt tctcggacgt cgtgaacggc cagaattatc
     2161 cgcgtaacgc tcgcatggat tcaggcggcg tttcgctggt attgacgtac gacttgaccg
     2221 accggacgag cgcgaagcta attttggccg accgcggtta cgacaccgag tggacgaacg
     2281 acagcgaccg cacgcccttc gggctaacgc aaacgcacta tgtccagaat catgacgagc
     2341 agcaggcgga gctgcagttc agcggtgcgg cgggtggcaa cgacaggctc ggttggaccg
     2401 ccggactgtt cttcttcgag tcggagagcc gcgcttacaa cacgaccgag ttcgaggcgt
     2461 tcaattacac gggcgggctg ttgaacttcg tcgccaatga cggctacacg acggacaacg
     2521 aatccgcttt cgtgcacgtc acctacgact tcacggagcg cctgcgcgta tcgggcggcg
     2581 cccgctacac ggacgagacg aagacgaaca ccttcgacca cggcccggcg ctgaatcgtt
     2641 ccgacgtgcc gctgatcttc ggcgactcgc gcaccgactg gaagctcagc gtggacttct
     2701 cgctgacgga ctcggtgttc ttgtacgcac aggcggctac ggggttcagc tcggagagcg
     2761 caacgccgcg catcttcacg gttggccagc tcatggcgct cgccggcgaa gagctgctga
     2821 gcgaggagat cggcgcgaag ctcgagttcc tcgacagccg cttgcgtgtc aacgcggcga
     2881 tcttcaccag cgactacgac ccgcgcatcc ggcagacggg cggcgtgaac cagtgcgatg
     2941 cgccgacgga gttgaacccc gtgccatacc ggctgcaggg cgggacgtgt cctcccggga
     3001 ccttctttgc cggctctacg ggattgccat ggttcttcta cgacaactcg ccgggcacgc
     3061 tcgacggtta cgaggtcgag ctgacggcga ctcccgtcga gcgattgctg atcagtttct
     3121 cgcttgggca gaacgagtac gaaaacacga acaacgatcc ggggccgagc aatttcacct
     3181 acatcgcgcc gggctatctg ttccagccgg agttcaactc gagcttggga ctccagtacg
     3241 acttccggtt cgggaacggc ggcacgctgt cgccgcggat cgacgcgttc tatcagtcgg
     3301 agcgccacac ggggcccgca aatgcgcggc cggcggctca cgaggtgacg gccaatacct
     3361 gtcctacgca gtgtatcgag gactacacga ccttcaacgc acgtctcaca tatcagccac
     3421 cgaacgcaga gtggcgtctg gcgatattcg gcacgaacgt gaccaacgag ttttactggc
     3481 agcagtactc gcccgagatc acggtcaacg ccacgacggg ggcgattacg aacacggcgc
     3541 cgttcggccg cacgggtgtg gcgagcgcgc cgcgagaatg ggcgttgac
//
LOCUS       DASSNN010000746         4402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1876555, whole genome shotgun sequence.
ACCESSION   DASSNN010000746 DASSNN010000000
VERSION     DASSNN010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876555"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFL84_14005"
     CDS             <1..286
                     /locus_tag="VFL84_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609790.1"
                     /translation="TGLLDFASLPRAVQDTRELTSHILCMAVAFIFFLGAALLRTRSG
                     SFATPAVWYVVAIESVGSLFLVYGGHLASIVVFERLRTAPLDDVTLQPKP"
     gene            complement(376..451)
                     /locus_tag="VFL84_14010"
     tRNA            complement(376..451)
                     /locus_tag="VFL84_14010"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(416..418),aa:Ala,seq:cgc)
     gene            complement(513..1424)
                     /locus_tag="VFL84_14015"
     CDS             complement(513..1424)
                     /locus_tag="VFL84_14015"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HET7609791.1"
                     /translation="MFFLTDALVIGLRAVVFVALFQAAGAALFLALYERQTAPAAAER
                     LRRFARAAALVALVAAVLHYVLTPARMAGSFGSTFDPSLESLLLRSSSGTAHIVRVVG
                     LAILLLSLDRASRVNTYGACIGAALALWSFALMGHTAIHPQRLILAPLLLVHVVVAAV
                     WLGALAGLYIAARETGTASAPLVARFSAHAGWAVPAIFVCGLGMSVIFIGSFAELATP
                     YGAMVLGKSLAFAALMALAAQNKWRLGPRLSAGDASAVPALRRTVKAEWVLIAVVLAG
                     TAVMTSLYAPEHLEGSFAPEHRDAPAH"
     gene            complement(1431..1784)
                     /locus_tag="VFL84_14020"
     CDS             complement(1431..1784)
                     /locus_tag="VFL84_14020"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HET7609792.1"
                     /translation="MKRLLGLALALLLAAVANAHTPLKESSPAADASVPAPKAIVLTF
                     GGDVRLTSVSLTDSTGAAKHLDAPPTATGAMFRIAVHEPLAPGAYKVVWRAVGGDTHI
                     VSGELGFTVVAAPAQ"
     gene            1924..3162
                     /locus_tag="VFL84_14025"
     CDS             1924..3162
                     /locus_tag="VFL84_14025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET7609793.1"
                     /translation="MPTDVRAESRRNFLRFLAESPLLLGLGGSLAAGTARAQDDAVLS
                     GALDFNGVIKSAADAVNVWDFEAAVRTKLNAGHYAYVAQGSDDFGTIAANRAAYRKIG
                     LRPRRLVDVGTIDMGVEVFGQRLSSPLMLCPVGAQLMFHAEGEVGVARAAKAKNALQI
                     LSTVTSMSVEDVAAARGGPGWFQLYTVSDWSATERMLKRAEDAGCTALAWTVDIPVRN
                     LEALERFDRDTDPVCQACHAGMPSDPFKMRRMFDGIDMSKVRMGLAGLSWSYIERLKG
                     ATKMKVLLKGIQTREDAALCLKYGVDGIVVSNHGGRADETLRGTLDCLPEVVEAVGGR
                     IPVFVDGGIRRGTDIFKALALGAKAVGIGRPYIWGLGSFGQQGVERVLDILTRELHIV
                     MTQMGAAKIADISKTSLQPL"
     gene            complement(3281..3691)
                     /locus_tag="VFL84_14030"
     CDS             complement(3281..3691)
                     /locus_tag="VFL84_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET7609794.1"
                     /translation="MYQSEIEITTKGRGTYELTARVAEAVRSARVRTGLCHVFTTHTS
                     ASLMICENADPDVRRDLETFMADLAPDGDPRFVHTAEGPDDMSAHVRSVLTTSDLTIP
                     IKDGELALGTWQGVYLWEHRRSGHRRCVLVTIQS"
     gene            3756..>4402
                     /gene="trmH"
                     /locus_tag="VFL84_14035"
     CDS             3756..>4402
                     /gene="trmH"
                     /locus_tag="VFL84_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HET7609795.1"
                     /translation="MTPERFQKLQRALDRRQPDLTVFMDAVNKPHNLSAIIRTADAVG
                     IQRLHAISEGEAIRRHHMIAGGAKHWVGITLHKSTKAALAALRAAGWRLVAAHLGEKT
                     RDYRDVDYTAKVALMVGAELVGLSDAAKAAADEHIAVPMHGLGTSLNVSVAVGVILAE
                     AERQRLKAGMYERSRLTPEEREQTLFEWSYPDIAERCRQLGRPYPKLTADGRLAA"
BASE COUNT          755 a         1461 c         1505 g          681 t
ORIGIN      
        1 gacgggtctt ctggatttcg catctctgcc gcgggccgtg caggacaccc gggagctgac
       61 ttcccacatc ctctgcatgg ctgtcgcctt cattttcttt ctcggcgcag cgcttctgcg
      121 cacgcgctcg ggctcgttcg caacgcctgc cgtgtggtac gtagtggcca tcgaatcggt
      181 cggctcgctg tttctcgttt atggcgggca tctcgcgtcg atcgtagtct tcgaacgact
      241 ccggacggct ccgctagacg acgtaacctt gcagccaaag ccgtgagccc atgcgcgagg
      301 cgaagccgat gacgtgctgc gagcgcagca cggcttcaac cccggggggc gatcaacaga
      361 atgtaggcgt cggattggtg gagcagatgg gggtcgaacc cacgacctcc gcattgcgaa
      421 cgcggcgctc tcccaactga gctactgccc cacgcgaggc gcgaattatc ggccagtttc
      481 cgcgccggcc gcaagccggg ccgcctgatt tctcaatgtg ccggcgcgtc ccggtgctcg
      541 ggcgcgaacg agccctcgag atgctcgggc gcgtacagcg acgtcatgac ggccgtgccg
      601 gcgaggacga cggcgatcag gacccactcg gctttcacgg tgcgccgcag cgcgggcacg
      661 gcgctcgcgt cacccgcgga cagtcgcggc ccgagccgcc acttgttctg cgcggcgagc
      721 gccatcagcg cggcgaacgc gagcgacttg ccgagcacca tcgcgccgta cggcgtcgcg
      781 agctcggcaa agctgccaat gaaaatcacg ctcatgccga ggccgcagac gaagatcgca
      841 ggcacggccc aacctgcgtg cgctgaaaag cgcgcgacga gcggcgcgct cgccgtgccc
      901 gtttcgcgcg cggcgatgta gaggcccgcc agcgcgccga gccaaaccgc cgcgacgacg
      961 acgtggacga gcagcagcgg cgcgaggatc aagcgctgcg gatgaatcgc ggtgtggccc
     1021 atcaacgcga acgaccacaa tgcgagcgcc gcgccgatgc acgcgccata cgtgttgacg
     1081 cggctcgcgc gatcgaggct gagcagcagg atcgcgaggc cgacgacgcg cacgatgtgc
     1141 gccgtgcccg agctcgagcg cagcagcagc gactcgagcg aaggatcgaa cgtgctgccg
     1201 aagctgccgg ccatgcgcgc aggcgtcagc acgtagtgca gcacggccgc gacgagcgcg
     1261 acgagcgccg cggcacgcgc gaatctgcgc agccgttccg cggcagccgg cgccgtctgg
     1321 cgttcgtaga gtgcgaggaa cagcgccgcg cccgcggctt ggaacaacgc gacgaagacg
     1381 acggctcgaa gaccgatgac gagcgcgtcg gtgagaaaga acacggcgag ctattgtgcg
     1441 ggcgccgcga ccaccgtgaa gccgagctcg cccgagacga tgtgtgtgtc gccgccgacc
     1501 gcacgccaga cgactttgta ggcgccgggt gcgagcggct cgtgaacggc gatccggaac
     1561 atcgcaccgg tggccgtcgg gggcgcgtcg aggtgtttcg ccgcacccgt ggaatccgtc
     1621 aacgaaaccg aggtcaggcg aacgtcgccg ccgaacgtga gcacgatcgc cttcggcgcc
     1681 ggcacggagg cgtcggccgc gggcgacgat tccttgagcg gcgtatgagc gttcgcgacg
     1741 gcggcaagca gcagcgcgag cgcaagaccc agcaaacgtt tcatcggtga gctcctcgaa
     1801 ggcgccattc tacgcgtgtg cgcgccggcg cgtcttgcgc ctccgcgcgg cgaaccggcc
     1861 cgtcttgggc gggccgcgag agcggtggta tgttggcccc cgaagctcac tgggggacct
     1921 ggaatgccga ccgacgttcg cgccgaatcg cgccgtaatt tcctgcgttt tctggccgag
     1981 agcccgctgc tgttgggact aggcggctct ctcgcggcgg gcacggcgcg cgcgcaggac
     2041 gacgcggtgc tgtcgggcgc gctcgacttc aatggcgtca tcaagagcgc cgcggatgcc
     2101 gtcaacgtct gggacttcga agcagccgtc cgcacgaagc tcaacgcggg ccactacgcg
     2161 tacgtcgcgc aaggctccga cgacttcggc acgatcgcgg cgaaccgcgc cgcgtaccgg
     2221 aagatcggct tgcggccgcg gcggctcgtc gacgtcggca cgatcgacat gggcgtcgaa
     2281 gtcttcggcc agcgcttgag ctcgccgctc atgctctgcc ccgtcggcgc gcagctcatg
     2341 tttcacgcgg aaggcgaagt aggagtcgcg cgcgctgcga aagccaagaa cgcgctgcag
     2401 attctctcga ccgtcacgag catgtccgtc gaggacgtcg ccgcggcgcg cggcggtccc
     2461 ggctggttcc agctctatac ggtgtcggat tggtcggcga cagagcgcat gctgaagcgc
     2521 gccgaggacg ccggctgcac ggcgctcgcg tggaccgtcg acattcccgt ccgaaatctc
     2581 gaagcgctcg aacgcttcga ccgcgacacc gatccggttt gtcaggcgtg ccacgccggc
     2641 atgccgtcgg atccgttcaa gatgcggcgc atgttcgacg gcatcgacat gtcgaaggtg
     2701 cgcatggggc tcgcgggctt gagctggagc tacatcgagc ggctcaaagg cgcgacgaag
     2761 atgaaggtgc tgctgaaagg cattcagacg cgcgaggacg cggcgctgtg tcttaagtac
     2821 ggcgtcgacg gcatcgtagt cagcaatcac ggcggccgcg ccgacgagac gctgcgcggc
     2881 acgctcgact gcctgcccga ggtcgtcgaa gccgtcggcg gccgcatccc cgtgttcgtc
     2941 gacggcggca tccgccgcgg caccgacatc ttcaaggcgc tcgcgctcgg cgcgaaagcc
     3001 gtcggcatcg gccggccata catctggggc ctgggctcgt tcggccagca aggcgtcgag
     3061 cgcgtgctcg acatcctgac gcgcgagctc cacatcgtca tgacgcagat gggcgcggcg
     3121 aagatcgccg acatctcgaa gacctcgctg caacccctct aaaaggggac agatttattt
     3181 ttcgctgaaa gtgtagatgt ctcgacttgg aacgtgccgc gaaaaataaa tccgtcccct
     3241 tttagctggg gcgtgccgcg aaaaataaat ctgtcccctt ttagctctgg atcgtgacga
     3301 gcacgcagcg gcgatggccg ctgcggcggt gctcccacag atagacgccc tgccacgtgc
     3361 cgagcgcgag ctcgccgtcc ttgatcggaa tcgtcaggtc cgacgtcgtc aagacgctgc
     3421 gcacgtgcgc ggacatgtcg tcgggccctt cggccgtgtg cacgaagcgc ggatcgccgt
     3481 cgggagcgag atcggccatg aatgtctcga ggtcgcggcg cacgtcggga tccgcgttct
     3541 cgcagatcat cagcgacgcg ctcgtgtgcg tcgtgaagac gtggcagagc cccgtgcgca
     3601 cgcgcgccga gcgcacggcc tcggcgacgc gcgccgtcag ctcgtacgtg ccgcggccct
     3661 tcgttgtgat ctctatctca ctctggtaca tgaggtcatt ctaggtagcg ccttgctgct
     3721 aggataccgg ccgtggggcc ggcaaggact gccttatgac acccgagcga ttccaaaagc
     3781 tccagcgcgc gctcgaccgc cgccaacccg atctcacggt gttcatggac gccgtgaaca
     3841 agccgcacaa tctgtccgcg atcatccgca cagcggatgc cgtcggcatc cagcggctgc
     3901 acgcgatctc ggaaggcgaa gcgatacgac gccatcacat gatcgcgggc ggcgcgaagc
     3961 actgggtcgg gatcacgctg cacaagtcga cgaaggcggc gctcgcggcc ttgcgcgccg
     4021 cgggttggcg gctcgtcgcc gcgcatctcg gcgagaagac gcgcgactat cgcgacgtgg
     4081 attacacggc gaaggtggcg ctcatggtcg gcgccgagct cgtaggcttg agcgacgcag
     4141 ccaaagccgc tgccgacgag cacatcgccg tcccgatgca cgggctcggc acgtctctga
     4201 acgtctcggt cgcggtcggc gtgatcctcg ccgaggccga acggcaacgg ttgaaggccg
     4261 gcatgtacga gcgctcgcgg ctcacgcccg aggagcgcga gcagacgctg ttcgaatggt
     4321 cgtatcccga tatcgcggag cgctgccggc agctgggccg cccttacccg aagctcacgg
     4381 ccgacggccg gctcgccgcg aa
//
LOCUS       DASSNN010000747         8342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_503188, whole genome shotgun sequence.
ACCESSION   DASSNN010000747 DASSNN010000000
VERSION     DASSNN010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503188"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFL84_14040"
     CDS             complement(<1..422)
                     /locus_tag="VFL84_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HET7609796.1"
                     /translation="MRVAAHEEALPAARYGSLADVAGRIEAMLGSGESAHWLLDRNQL
                     ALEARLALVDEAAESLDVQYFVWQSDASGHLLADRMLHAADRGVRIRILIDDFGVSGK
                     GGDVLKLDAHPNIEVRNFNPWSTRGNRLGTSTEFLARA"
     gene            651..2129
                     /locus_tag="VFL84_14045"
     CDS             651..2129
                     /locus_tag="VFL84_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013654675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7609797.1"
                     /translation="MSGKLDLFIGGQWVAAKNKAKFPVYNPSTGEVFAEVADAGRADA
                     AAAIEAAAAAQPAWAALPHSKRAALLTKVADVLEARAKEFQAALIDEGGAWVGKTMFE
                     TGYSVGVYRAAAAAAYQVTGEILPSDHGKLSLVVREPLGVVSVISPWNFPLILSSRGF
                     AVALAVGNTVVLKPSEETPVSGGLLIAEVLEEAGIPKGVFNVVTCSRDNVAAVGDELI
                     SNPTVRGISFTGSTAVGSQVAAKAGGLLKKCCVELGGKDALIVLDDADIEHAVNAATF
                     GSFMHQGQICMAVERVIVHKDVANEFIEKFVQNTKKLKVGNPREMANCIGPIINQKQL
                     DKIRDQVEDAVAKGAKVLCGGKHKGLFFEPTVLTNVTRDMKVMREETFGPVAPVITVG
                     SVDEAVAVANDSEYGLSAGIMTRNEERGLAIARRLNTGMAHINDSSVNDEPHVPFGGV
                     RWSGLGRHGGRLGIDQFTEMRWITLERGGRHYPPPFLVNPGH"
     gene            2279..2734
                     /locus_tag="VFL84_14050"
     CDS             2279..2734
                     /locus_tag="VFL84_14050"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609798.1"
                     /translation="MVKQVVKQVLPTTMVGSYPRPSWFKQQLLGRDIRVAFKEVAHEE
                     TYQDAVATVIRDQIDSGLDIVTDGNMWYDDYVGVIGSFCWYLYERIPGFEPTRNPHPS
                     MVNAAPSAGKTFLDDWAASSTTAPSTRSPIACVGPISTTSPRKPPATSR"
     gene            2629..3402
                     /locus_tag="VFL84_14055"
     CDS             2629..3402
                     /locus_tag="VFL84_14055"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609799.1"
                     /translation="MGGVINNGPVDKEPDRLRWPDLYDIAQKAAGDVPVKCSIGAGPA
                     NLAWHVYFNNQSYYKSPKDLTFALCPIFNKEMKELVKHGAKYLQIEDLGAWLPLFTGN
                     KDDYKWIRESIEMMCDGVDAKIGWHFCFGNAWGNDILSKNFPQGYQTVLPHFWNTEGV
                     DEFVLDYANRHMAGVEFLKNCPKNKGVQVGVLDIRTSMVETPDDVADRIRTTLKYLSP
                     EQVTLSTDCGMKPLARMVSKIKLNALAEGAAIVRKEIKA"
     gene            complement(3485..4609)
                     /locus_tag="VFL84_14060"
     CDS             complement(3485..4609)
                     /locus_tag="VFL84_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609800.1"
                     /translation="MKPGILNSVLVEQVRNTAAWLWADPSSSALTAIFDAARDEPLDA
                     PGALERTLRLRLAAHYATVATFVPTDVDAHIRHHMWLSVGDAETFARAKACIDEAVAR
                     DARLVSARVTRGLSGHDGEWLSVRAGALGRALVLGLAEAAEVLAAEIDAELERERRAF
                     AEALEQGAPATVVLALATTIAHNLGDLSRVVDAWPKVARASEHCARYSRLGHSDGARA
                     GAEFLVAGALNKELMALENHRFLALRKPRALRRSRALLLPVGPWFDAWGETVCRSELL
                     DDADRAEVVAALVELHLRSPAQEGCLRALAAFHRSTRGGLAWYDTALPARLRKEIGRG
                     RIREALDVSRERFEARIEGRCRTLIARLTDGASQRLRASA"
     gene            complement(4752..6092)
                     /locus_tag="VFL84_14065"
     CDS             complement(4752..6092)
                     /locus_tag="VFL84_14065"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET7609801.1"
                     /translation="MFITKKHLDRRTFLRGAFGTAIALPLLDAMVPAATAQTRTAAAE
                     RLRFGAIYMPNGVYPGTWHPTKVGKDFEFNRTMKPLEPFRDNLTTISKLKAPEGKKDM
                     GGIHMGASAAFLNGRGPVGHNGDFNLIESKKSIDQYIADAIAGDTPLRSLELGTEDMG
                     TSAGACDGYPCVFFNTISWYDDSSPLPVSVNPQATFERMFGDPGTPEQRLHRLQAKES
                     MLDSVLQETHRLQKVIGAKDNAILDEYMTNIRRVEEQLQKMQARSGALTNAADGPVGI
                     PEDFNEHVTITYDLLHIAFQGDISRVFTFMVGHEGSGRSYAHVGVKEPHHSTSHHKNT
                     PESIEQYARITAYHMVKLAEFLTKLKNTPEGDGTLFDTAMVYFGAGMSNGLLHDRHNV
                     PAALIGSANGRLKGNRHLEARVDQPTSDLLLGMADLLGAEVETIGIAEGRFNLA"
     gene            complement(6096..>8342)
                     /locus_tag="VFL84_14070"
     CDS             complement(6096..>8342)
                     /locus_tag="VFL84_14070"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET7609802.1"
                     /translation="DNVHTDPAVWEEVLRKLKIHAMPPRMERQPPAEMVAEFVSGLEG
                     TLDAAAAANPYAGDTKVHRLNRAEYANAVRDVLGVEADLSSLLPSDGGDFGFDNIAEV
                     LTTSPLLLERYLTVALRVADMAVGNPEAAVTATAYRIPCELTQDKHLDGMPLGTRGGT
                     RVTHTFPADGEYVFSGRLVRGVEEGLFGIEGHDRPHEFLILVDGNTVYSAEVGGAELH
                     ELSVKEGINVAQDSVNAAMTSPPIPIAAGPHEVAFTWRERSPSEQNAWEPGLRASLEA
                     HNPSGMPRLEDGVVAGPYNVTGVSETPTRKRIFVCRPASAAEEPACAEQILSSLARRA
                     FRRSVEADDIQASLAFYNDARAAGGDFDDGIRAAVSRMLVSPWFLFRVEGDSPDVPAG
                     SNHPVTDFELASRLSFFLWSTIPDDELLTLAENGRLREPKTLEAQVRRMIADSRSDAM
                     VENFVGQWLQLRNLESRVRPDFLLFPDFDDNLRKSFRKETELLFANVLREGRPVQELI
                     TADYTFVNERLARHYGMKGVYGARFRKVELTDPNRYGLFGHGSLLSLTSVSTRTSPII
                     RGKFIQTEFWNNPPPTPPADVPALEESAPKDRPSTVREQLELHRANPVCAACHNNIDP
                     VGFALENFDADGSWREKTREGLEIDSAGILLDGTPVNGPVELRGALLADPNLFASTVA
                     EKMLIYALGRGLQPADKPIVRQIVRNAAENDYSLVSIVLGIVDSYPFQNRTNGGAAAT
                     ATIAQTRE"
BASE COUNT         1406 a         2752 c         2819 g         1365 t
ORIGIN      
        1 tacgcacgcg ccaggaactc ggtcgacgtg ccgagacggt tgccgcgcgt gctccacggg
       61 ttgaagttgc gcacctcgat gttcgggtgc gcgtcgagct tcagcacgtc gccgcccttg
      121 cccgagacgc cgaaatcgtc gatcaggatt cgaatccgga cgccgcgatc ggccgcatgc
      181 agcatccggt cggcgagcag atggccgctc gcgtcgctct gccaaacgaa gtactgcacg
      241 tcgagcgact cggccgcctc gtccacgagg gcgagccgcg cttccagcgc gagctggttg
      301 cgatcgagca gccaatgcgc cgattcgccg ctgccgagca tcgcctcgat gcggcccgcg
      361 acgtcggcca gcgacccgta gcgagccgcc ggcaaggcct cctcgtgcgc ggcgacgcgc
      421 acgggcaagg ctcggcagcc ggcgaccgca aagctgacga cggcgattac gagggcggcg
      481 gctctgatca cgccccggcg gcgcgcctgc gcacggtgtg tgctttggtt atcgacccca
      541 ctcgttgtct atactagcgc ggcgaagccc aatgcaagca acaacagggg ccggtacacg
      601 ggtgcttcgc gcggcggaac aatcgagcgc cccaccttag gagaacgcgc atgagcggca
      661 agctggatct attcatcggc ggtcagtggg tcgccgcgaa gaacaaagcg aaattccctg
      721 tctacaaccc ttcgaccggc gaggtcttcg ctgaagtggc cgatgcgggc cgggccgatg
      781 cggccgccgc gatcgaagcc gccgcggccg cgcagccggc ttgggccgct ctgccgcatt
      841 cgaagcgcgc ggcgctgctc acgaaggtcg ccgacgtgct cgaggcgcgc gccaaagaat
      901 tccaggcggc gctgatcgac gagggcggcg cgtgggtcgg caagacgatg ttcgagacgg
      961 gctacagcgt cggcgtatac cgagcggcgg ccgcggccgc gtaccaggtc acgggcgaga
     1021 tcttgccatc cgatcacggc aagctgagcc tcgtcgtgcg cgagccgctc ggcgtcgtct
     1081 cggtcatctc gccgtggaac ttcccgctga tcttgtcgtc gcgcggcttc gccgtcgcgc
     1141 tcgcggtcgg caacaccgtc gtgctgaagc cgtccgagga gacgcccgtc tcgggcgggc
     1201 tcctgatcgc cgaggtgctc gaggaagccg gcatcccgaa aggcgtgttc aacgtcgtca
     1261 cctgctcgcg cgacaacgtc gcggccgtcg gcgacgagct catttcgaat ccgaccgtgc
     1321 gcggcatcag cttcacgggc tcgacggccg tcggcagcca ggtcgcggcg aaggccggcg
     1381 ggctgctcaa gaagtgctgc gtcgagctcg gcggcaagga cgcgctgatc gttctcgacg
     1441 acgcggacat cgagcacgcc gtcaacgccg cgacgttcgg ctcgttcatg caccaaggcc
     1501 agatctgcat ggcggtcgag cgcgtgatcg ttcacaagga cgtcgcgaac gagttcatcg
     1561 agaagttcgt gcagaacacg aagaagctca aagtgggcaa cccgcgcgag atggcgaact
     1621 gcatagggcc gatcatcaac cagaagcagc tcgacaagat ccgcgaccag gtcgaggacg
     1681 ccgtcgcgaa gggcgcgaag gtgttgtgcg gcggcaagca caagggcttg ttcttcgagc
     1741 ccaccgtgct cacgaacgtc acgcgcgaca tgaaggtcat gcgcgaggag acgttcggac
     1801 ccgtggcacc cgtgatcacg gtcggaagcg tcgacgaggc cgttgccgtc gcgaacgact
     1861 ccgagtacgg gctctcggcc ggcatcatga cgcgcaacga ggagcggggt ctcgcgatcg
     1921 cgcggcggct caacacgggc atggcgcaca tcaacgacag ctcggtcaac gacgagccgc
     1981 acgtgccgtt cggcggagtc cgctggagcg gcctaggccg gcacggcggg cgcttaggga
     2041 tcgatcagtt caccgagatg cggtggatca cgctcgagcg cggcggacgg cactacccgc
     2101 cgccgttcct cgtgaacccg gggcactgag cgcggcgacg aaggcgtttc gggcggcgcg
     2161 ccgcgaccgc gccgaaacgc cgccaaacca cgcggtgcgc ggctatccgg gcgggaattg
     2221 catggcttat actgcggccc ctcatcacga gctggcgctg attcagtagg agaaaaaaat
     2281 ggtaaagcaa gtcgtaaaac aagtgctccc gacgacgatg gtcgggtcct atccccggcc
     2341 gagttggttc aagcagcagc tgctgggtcg tgacattcgc gtcgcgttca aggaggtcgc
     2401 ccacgaggag acgtaccagg acgcggtcgc caccgtcatc cgcgatcaga tcgatagcgg
     2461 cctcgacatc gtgaccgacg gcaacatgtg gtacgacgac tacgtcggcg tcatcggctc
     2521 gttctgttgg tacctctacg agcgcattcc gggcttcgag ccgacgcgca atccgcaccc
     2581 gtcgatggtc aatgcggccc cgagcgccgg caagacgttc ctcgacgatt gggcggcgtc
     2641 atcaacaacg gccccgtcga caaggagccc gatcgcctgc gttggcccga tctctacgac
     2701 atcgcccaga aagccgccgg cgacgtcccg gtgaagtgct cgatcggcgc cggcccggcc
     2761 aacttggcct ggcacgtcta cttcaacaat cagtcgtact acaagagccc gaaggacctg
     2821 accttcgcgc tctgcccgat cttcaacaaa gagatgaagg agctcgtcaa gcacggcgcg
     2881 aagtacctgc agatcgagga cctcggcgcc tggctgccgc tcttcacggg caacaaggac
     2941 gactacaagt ggatccgcga gagcatcgag atgatgtgcg acggcgtcga cgcgaagatc
     3001 ggctggcact tctgcttcgg caacgcctgg ggcaacgaca tcctgagcaa gaacttcccg
     3061 cagggttacc agaccgtgct gccgcacttc tggaacaccg agggcgtcga cgaattcgtg
     3121 ctcgactacg cgaatcgcca catggccggc gtcgagttcc tgaagaactg cccgaagaac
     3181 aagggcgtgc aggtcggcgt gctcgacatc cgcacgagca tggtcgagac gccggacgac
     3241 gtcgccgatc gcatccgcac aacgctgaag tacttgtcgc cggagcaagt cacgctatcc
     3301 acggactgcg gcatgaagcc gctcgcgcgc atggtgtcga agatcaagct caacgcgctg
     3361 gccgaaggcg ccgcgatcgt ccgcaaggag atcaaggcct aaaagccgtc gcgaactttc
     3421 gcgattcgaa cgaacgggcc ggcctcgaaa gccggcccgt tttttttctg ctcctggctc
     3481 ggcgttaggc gctcgcgcgc agccgttgcg acgcgccgtc cgtgaggcgc gcgatcagtg
     3541 tgcggcagcg gccttcgatg cgcgcttcga agcgctcgcg cgagacgtcg agagcctcgc
     3601 ggatgcggcc gcggccgatc tccttgcgca ggcgcgcggg cagcgccgtg tcgtaccagg
     3661 cgaggccgcc gcgcgtagag cggtggaacg cggccagagc gcgcaagcat ccttcctgcg
     3721 ccggagagcg caggtgcagc tcgacgagcg ccgcgacgac ctcggcgcgg tcggcatcgt
     3781 cgagcagctc gctgcggcag accgtctcgc cccaggcgtc gaaccagggg ccgaccggca
     3841 gcagcagcgc gcggctgcgg cgcagcgcgc gcggcttgcg cagcgccagg aaccggtggt
     3901 tctcgagcgc catgagctcc ttgttcaacg cgccggcgac gagaaactcg gcgcccgccc
     3961 tcgcgccatc cgagtgcccg agccgcgaat agcgcgcgca gtgctcgctc gcgcgggcca
     4021 ccttcggcca ggcgtcgacg acgcgcgaaa ggtctccgag gttgtgcgcg atcgtcgtcg
     4081 cgagcgcgag caccaccgta gcgggcgcac cttgctcgag cgcctcggca aaggcgcgcc
     4141 gctcgcgctc gagctcggcg tcgatctcgg cggccagcac ttccgccgct tcggcgagtc
     4201 ccagcacgag cgcgcggccg agcgcgccgg cgcgaacgga cagccattcg ccgtcgtgcc
     4261 cggaaaggcc gcgggtgacg cgtgccgaca cgagacgagc gtcgcgcgcg acggcctcgt
     4321 cgatacaggc tttggcgcga gcgaacgtct cggcgtcgcc gacgctgagc cacatgtgat
     4381 ggcggatgtg cgcgtcgacg tcggtcggca cgaacgtcgc gacggtcgcg taatgcgccg
     4441 ccagacgcag ccgcagcgtt cgctcgagcg cgccgggggc gtcgagcggc tcgtctcgcg
     4501 ccgcgtcgaa aatcgcggtc agcgcgctcg aggaaggatc ggcccagagc cacgcggccg
     4561 tgttgcgaac ctgctcgacg agaaccgaat tcaggatgcc gggcttcact tccgctgcga
     4621 ctgctctaca gacaaagacg ccgcctcgga ggcggcgtcg ttcggaatcg aatccgggga
     4681 cggattgata aatccgtccc ctcgtcgagg tccggggacg gatttatcaa tccgtcccct
     4741 cttcgcatgt cctacgcgag gttgaagcga ccctcggcaa tgccgatcgt ctcgacctcg
     4801 gcgccgagca gatcggccat gccgagcaag agatccgacg tcggctgatc gacgcgcgcc
     4861 tcgagatggc gattgccctt caagcggccg ttggcgctgc cgatcagcgc ggccggcacg
     4921 ttgtgccggt cgtgcaagag accgttgctc atgcccgcgc cgaagtaaac catcgccgtg
     4981 tcgaacagcg tgccgtcgcc ttccggcgtg ttcttgagct tcgtcaagaa ctcggcgagc
     5041 ttgaccatgt ggtacgccgt gatgcgcgcg tattgctcga tgctctccgg cgtgttcttg
     5101 tggtgcgacg tcgaatggtg cggttccttg acgccgacgt gcgcatagct gcgaccgctg
     5161 ccctcgtggc ccaccatgaa cgtgaacacg cgcgagatgt cgccctggaa ggcgatgtgc
     5221 agcaagtcgt acgtgatcgt gacgtgctcg ttgaagtcct cggggattcc gacggggccg
     5281 tccgcggcgt tcgtcagcgc gcccgagcgc gcctgcatct tctgcagctg ctcttcgacg
     5341 cggcggatgt tcgtcatgta ctcgtcgagg atcgcgttgt ccttggcgcc gatgaccttc
     5401 tgcagccgat gcgtctcctg cagcacggaa tcgagcatgc tctccttcgc ctgcaagcga
     5461 tgcaggcgct gctccggcgt gcccgggtcg ccgaacatgc gctcgaacgt ggcctgcgga
     5521 ttgaccgaga ccggcagcgg gctcgagtcg tcgtaccacg agatcgtgtt gaagaacacg
     5581 cacgggtagc cgtcgcaggc gccggccgac gtgcccatgt cctcggtgcc gagctcgagc
     5641 gagcgcagcg gcgtgtcgcc ggcgatcgcg tcggcgatgt actggtcgat cgacttcttc
     5701 gactcgatca ggttgaagtc gccgttgtgg ccgaccgggc cccggccgtt caagaacgcg
     5761 gcgctggcgc ccatgtggat gccgcccatg tccttcttgc cttcgggcgc cttcagcttg
     5821 ctgatcgtcg tcaagttgtc acggaacggc tcgagcggct tcatcgttcg gttgaactcg
     5881 aagtccttgc cgaccttcgt cggatgccac gtgccggggt agacgccgtt cggcatgtag
     5941 atcgcgccga aacgcaatct ctcggcggcg gcggtccggg tctgcgcggt cgcggcgggt
     6001 accatcgcgt cgagcagcgg cagggcgatt gcagtgccga atgcaccgcg caaaaacgtg
     6061 cggcggtcca aatgcttctt cgtgatgaac attggctact ccctcgtttg cgcgattgtc
     6121 gctgtcgcgg cagctccgcc attcgtccgg ttctggaacg gatagctatc tactataccc
     6181 aatacaatag agacaaggct gtaatcgttc tcggctgcat ttctcacgat ttgcctgacg
     6241 atgggcttgt cggcgggctg taaaccgcga cccagcgcat aaatcagcat tttttcggca
     6301 accgtgctcg cgaacagatt cggatcggcc agcaacgcgc cgcgcagctc gaccggcccg
     6361 ttgacgggcg tgccgtcgag caggatgccc gccgaatcga tttcgaggcc ctctcgtgtc
     6421 ttctcgcgcc agctgccgtc cgcgtcgaaa ttctcgagcg cgaagccgac cgggtcgatg
     6481 ttgttgtggc acgcggcgca cacgggattc gcgcggtgca gctcgagctg ctcgcggacc
     6541 gtggacggcc ggtccttcgg cgcgctctcc tcgagcgcgg gcacgtcggc cggcggcgtc
     6601 ggcggcggat tgttccagaa ctcggtctgg atgaacttgc cgcggatgat cggcgacgtg
     6661 cgcgtcgaca ccgacgtcag cgatagcagg ctgccgtgcc cgaagaggcc gtagcggttc
     6721 ggatccgtca gctcgacctt gcggaaccgc gcgccgtaca cgcccttcat gccgtagtgg
     6781 cgagcgaggc gttcgttgac gaacgtgtag tccgcggtga tgagctcctg cacggggcgc
     6841 ccttcgcgca gcacgttcgc gaacaagagc tcggtttcct tgcggaagga cttgcgcaag
     6901 ttgtcgtcga agtccgggaa cagcaagaag tccggcctga cgcgcgactc gaggttgcgc
     6961 agctgcagcc actgaccgac gaagttctcg accatcgcgt cggagcgaga atccgcgatc
     7021 atgcggcgaa cctgcgcctc gagcgtcttc ggctcgcgca gccggccgtt ctccgcgagc
     7081 gtcaagagct cgtcgtcggg aatcgtcgac cacaggaaga acgacaaccg cgaggcgagc
     7141 tcgaagtccg tgaccggatg gttcgagccg gccggcacgt cgggcgagtc gccttccacg
     7201 cggaacagga accaggggct cacgagcatg cgcgagaccg cggcacggat gccgtcgtcg
     7261 aagtcgccgc ctgcggcgcg cgcgtcgttg tagaacgcga gcgacgcctg gatgtcgtcc
     7321 gcttcgaccg agcggcggaa cgcgcggcgc gccagcgacg acaggatctg ctcggcacac
     7381 gccggctctt cggctgcgct cgcgggccgg cagacgaaga tgcgcttgcg cgtcggcgtc
     7441 tcgctgacgc ccgtgacgtt gtaaggtccg gcgacgacgc cgtcctcgag ccgcggcatt
     7501 ccggacgggt tgtgcgcctc gagcgaagcg cgcagtcccg gctcccaggc gttctgctcg
     7561 cttgggcttc gctcgcgcca cgtgaacgcg acctcgtgcg gcccggccgc gatcggaatc
     7621 ggcggcgagg tcatcgccgc gttgacggag tcctgcgcga cgttgatgcc ttccttgacg
     7681 ctgagctcgt gaagctctgc gccgccgacc tcggcggagt acacggtgtt gccgtcgacg
     7741 agaatcagga actcgtgcgg ccggtcgtgc ccttcgatgc cgaacaaacc ttcctcgacg
     7801 ccgcgcacga gccggcccga gaacacgtac tcgccgtcgg cgggaaacgt gtgcgtgacg
     7861 cgcgtgccgc cgcgtgtgcc gagcggcatg ccgtcgagat gcttgtcctg cgtcagctcg
     7921 cacgggatgc ggtacgccgt ggccgtcacg gcggcctccg gattgccgac ggccatgtcc
     7981 gcgacgcgca gcgcgaccgt gagatagcgc tcgagtaaga gcggcgacgt cgtcagcacc
     8041 tcggcgatgt tgtcgaagcc gaagtcgccg ccgtcgctcg gcagcaatga cgacaggtcc
     8101 gcctctacgc cgagcacgtc gcgaacggcg ttcgcgtact cggcacgatt caagcgatgg
     8161 actttcgtgt cgccggcgta cggattcgcg gcggccgcgg catcgagcgt gccctcgagg
     8221 cccgagacga actccgcgac catctcggcg ggcggctggc gctccatgcg cggcggcatc
     8281 gcatggatct tgagcttgcg cagcacctct tcccagaccg cgggatcggt gtgcacgttg
     8341 tc
//
LOCUS       DASSNN010000748         3332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1259071, whole genome shotgun sequence.
ACCESSION   DASSNN010000748 DASSNN010000000
VERSION     DASSNN010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259071"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VFL84_14075"
     CDS             <1..910
                     /locus_tag="VFL84_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7609803.1"
                     /translation="EQLAWAYAITPGPAPPAPADDGTRYSLDGSEGHFTLDQIRNRMG
                     PADWYPGDHPPMPPIVAKGREAAGIWACSLCHYPNGKGRPENAGVAGLPKQYFIQQMH
                     DFKNGLRASADSRKPNTPIMAGFAVQMTDEEIEQAADYFGAMRWTPWIQVVETDTVPK
                     TRISGGMHLKLEGAEAGTEPLGNRIVESPVNAEHTELLRDPRSSFIAYVPKGSVARGK
                     ELALYGNEGVTACTVCHGQDLNGLAIVPSLRGRSPSYIARQLADMKAGKRNGAWTPLM
                     APVVASLGSDDILNLAAYLASLEPMP"
     gene            complement(926..1885)
                     /locus_tag="VFL84_14080"
     CDS             complement(926..1885)
                     /locus_tag="VFL84_14080"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HET7609804.1"
                     /translation="MGFRIRHARAAAAAMLCAAAPIAAQPLKPGFADALLGRWDLAIE
                     APDGVYPSWLEVRLRTETELMGRFVGRVGSARYVSDIDYRDGRVTLRVPVQYETDIDA
                     LRFEGTVRGDRIEGTTLAADGAAVRFTATRAPALASQSRPRWRPAQPLANGRDLAGWT
                     ARNAQHPGCWRVQGAVLAATPPCVDLVSERTFGDFRLHAELRFPPGSNSGIYLRGRYE
                     VQIQDDAGKALDSLRMGGIYGFIAPGVDAARAAGEWQTLDVELVGRRVTVVLNGTTIV
                     DGQEIPGITGGALDSDEGAPGPIMLQGDHGPIEFRNLRIATAR"
     gene            1973..2743
                     /locus_tag="VFL84_14085"
     CDS             1973..2743
                     /locus_tag="VFL84_14085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7609805.1"
                     /translation="MRPMRFLASALALLVLAQTAAQAQAYRVPANAPANIKRAVESPS
                     RPADQRARDADRKPAETLMLANIAEGQRVVELAAFGHYWTNMLVEAVGPSGHVYMVDM
                     PWTDRFGGEAARAFDAAHENATYTQAHYNEMQLPQNVDAVIFVQFYHDLKRSNASETV
                     DTADMNKKIFAALKPGGIYLVVDHKAEAGSGWRDAMTLHRIDPATIKSEVTAAGFELV
                     QDSPLLANPADDVKQSMRAEGIRGKTDQAVLVFRKPAR"
     gene            2748..>3332
                     /locus_tag="VFL84_14090"
     CDS             2748..>3332
                     /locus_tag="VFL84_14090"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HET7609806.1"
                     /translation="MTALVRPFAALRPTADTAAAVVAPPYDVVSTDEARALAAGRPSS
                     FLHISRPEIDLPEGSSPYSDEAYAQGARSLARLVEKRVLVRDDAPSFYVYRMVMNGRA
                     QTGVAFGASVRAYEEHRIRRHELTRPDKEDDRVRNIASLNAQTGPVLLAYRANPALHE
                     LVGAAARLEPLFSVAGPNDVEHTIWRVTAPSEIAA"
BASE COUNT          551 a         1204 c         1121 g          456 t
ORIGIN      
        1 cgagcagctc gcctgggcct acgcgatcac gccgggcccc gccccgccgg cgcccgccga
       61 cgacggaacg cgctactcgc tcgacggcag cgaggggcac ttcacgctcg accagatccg
      121 caatcgcatg gggcccgccg actggtaccc cggcgaccac ccgcccatgc cgcccatcgt
      181 cgcgaaaggg cgcgaggccg ccggcatctg ggcctgctcg ctgtgccatt acccgaacgg
      241 caaagggcgc cccgagaacg ccggcgtggc cgggctgccg aagcagtact tcatccagca
      301 gatgcacgac ttcaagaacg gcctgcgcgc gagcgccgac tcgcgtaaac cgaacacgcc
      361 gatcatggcg ggcttcgccg tgcagatgac cgacgaggag atcgagcaag cggccgacta
      421 cttcggcgcg atgcgctgga cgccgtggat tcaagtcgtc gagaccgaca ccgtgccgaa
      481 gacgcgcatc tcgggcggca tgcacctgaa gctcgaaggc gccgaggccg gcacggagcc
      541 gctcggcaac cgcatcgtcg agtcgcccgt gaatgccgag cacaccgagc tcctgcgcga
      601 tccgcgctcg agcttcatcg cctacgtgcc gaaaggctcc gtcgcgcgcg gcaaggagct
      661 cgcgctgtac ggcaacgaag gcgtgaccgc gtgcacggtc tgccacggcc aggacttgaa
      721 cggcctcgcg atcgtgccga gcctgcgcgg ccgcagcccg agctacatcg cgcgccagct
      781 cgccgacatg aaggccggca agcgcaacgg cgcgtggacg ccgttgatgg cgcccgtcgt
      841 cgcgagctta ggaagcgacg acatcttgaa tctcgcggcg tatctcgctt cgctcgagcc
      901 gatgccgtag ccgttcgagc gcggctcagc gcgcggtcgc gatcctgaga ttgcgaaact
      961 cgatcggccc gtgatcgccc tgcagcatga tcgggcccgg cgcgccctcg tcgctgtcga
     1021 gtgcgccgcc cgtgatgccg ggaatctcct ggccgtcgac aatcgtcgtg ccgttcaaga
     1081 cgacggtcac gcggcggccg acgagctcca cgtcgagggt ttgccattcc ccggcggcgc
     1141 gggccgcgtc gacgcccggc gcgatgaagc cgtagatgcc gcccatgcgc agcgaatcga
     1201 gcgccttgcc cgcatcgtct tggatctgca cctcgtagcg cccgcgcaaa tagatgccgc
     1261 tgttgcttcc gggcgggaac cggagctccg cgtgcaagcg aaagtcgccg aacgtgcgct
     1321 cgctgacgag gtcgacgcac ggcggtgtcg cggcgagcac cgccccttgc acgcgccagc
     1381 agccagggtg ctgcgcgttg cgcgccgtcc accccgcgag atcgcggccg ttcgctagcg
     1441 gctgagcggg ccgccaccgc ggccgcgact gcgacgcgag cgccggcgcg cgcgtggccg
     1501 tgaaccgcac tgccgcgccg tcggccgcga gcgtcgtgcc ctcgatgcga tcgccgcgca
     1561 ccgtgccttc gaagcgcagc gcgtcgatgt cggtctcgta ctgcaccggc actcgcagcg
     1621 tcacgcggcc gtcgcggtag tcgatgtcgc tgacgtagcg cgcgctgccg acgcggccga
     1681 cgaaccggcc catgagctcg gtctcggttc gtagccggac ctcgagccag gacggataaa
     1741 cgccgtcggg cgcctcgatc gcgagatccc accggccgag cagcgcgtcg gcgaagccgg
     1801 gcttgagcgg ctgggcggca atcggcgccg ccgcgcagag cattgcggcc gccgcggctc
     1861 gcgcgtgccg aatcctgaac cccattcgcg aatgatacgc cctgcccgcc tgtagccgcg
     1921 caacagcgga actggtagaa cgcacgaagc tcattcgaag ggagaaagat ccatgcgccc
     1981 gatgcgtttc cttgcaagcg cgctcgccct gctcgtcctc gcccaaaccg cggcgcaggc
     2041 ccaggcctat cgcgtgccgg ccaacgcacc cgccaacatc aagcgcgccg tggaatcgcc
     2101 gtcgcgcccc gccgatcagc gcgcgcgcga cgcggaccga aagccggccg agacgctgat
     2161 gctcgcgaac atcgccgaag gtcagcgcgt cgtcgagctc gcggccttcg gccattactg
     2221 gacgaacatg ctcgtcgaag ccgtcgggcc ctccggccac gtctacatgg tcgacatgcc
     2281 gtggacggac cggttcggcg gcgaagccgc gcgcgcgttc gacgcggcgc acgaaaacgc
     2341 gacttacacg caagcgcact acaacgagat gcagctgccg caaaacgtcg acgccgtgat
     2401 cttcgtgcag ttctaccacg acttgaagcg cagcaacgcg agcgagaccg tcgacactgc
     2461 ggacatgaac aagaagatct tcgcggcgct gaagccgggc ggcatctacc tcgtcgtcga
     2521 tcacaaggcc gaggccggct cgggctggcg cgacgcgatg acgctgcacc gcatcgatcc
     2581 cgcgacgatc aagagcgagg tgacggccgc cggattcgag ctcgtgcagg acagcccgct
     2641 gctcgcgaat ccggccgacg acgtgaagca gagcatgcgc gccgaaggca ttcgcggcaa
     2701 aacggatcag gccgtgctcg tgttccgcaa gccggcacgt tagcgccgtg accgcgctcg
     2761 ttcgcccgtt cgccgcgctg cgcccgaccg cggacacggc ggccgccgtc gtcgcgccgc
     2821 cgtacgacgt cgtcagcacg gacgaagcgc gcgcgctcgc cgcgggccgg ccgtcgagct
     2881 tcctgcacat ctcgcggccg gagatcgatt tgcccgaggg cagctcgccg tattccgacg
     2941 aggcttacgc gcaaggcgcg cgcagcctgg cgcggctcgt cgagaagcgc gtgctcgtcc
     3001 gcgacgacgc gccgtcgttc tacgtttatc gaatggtcat gaatgggcgc gcgcagaccg
     3061 gcgtcgcgtt cggggcctcg gtgcgcgcct acgaagagca ccgcattcgc cgccacgagc
     3121 tcacgcgtcc cgacaaggag gacgatcgcg tgcgcaacat cgcatcgttg aacgcgcaga
     3181 cgggtcccgt gctcttggcg tatcgcgcga accccgcgct gcacgagctc gtcggcgcgg
     3241 ccgctagact cgagccgctg ttctcggtcg ccgggccgaa cgacgtcgag cacacgatct
     3301 ggcgcgtgac ggcgccgagc gagatcgctg cg
//
LOCUS       DASSNN010000749         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_503369, whole genome shotgun sequence.
ACCESSION   DASSNN010000749 DASSNN010000000
VERSION     DASSNN010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503369"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VFL84_14095"
     CDS             complement(<1..1207)
                     /locus_tag="VFL84_14095"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET7609807.1"
                     /translation="MAANEKAREATSAAAGASAQLGHVVAIGASAGALEALRTVLGGL
                     EASSSATFVVAVHRSSSSGGRLDGLLQPYTELPVREAAREELLEPHTILLVPASAEVG
                     VIDSHLRLVDMPAAEARRGRIDRLFRAVAAGRGSRGVGVLLTGAGSDGSLGLGGIKDR
                     GGLTIVQDPAEAEYAGMPSSALDSGVVDLVLPLRDIPSAIERYCGTDPQLKHDRREQG
                     TDESALFGELLALLEQRTGRDFAVYRQSLLAQRVAKRMRIHAIDDWPTYLGLLRTDTA
                     EAAALANELLLNVTEFFGHERKFERLESDVLPRLFATKTRDRDTFRAWVVGCSTGEDA
                     YSLAIALLEQRARRDVRPSIQVFASDMSDESLQRARSGFYPAEIEGLVSPERLAAFFV
                     RDEDRGYRVR"
     gene            complement(1283..>2027)
                     /locus_tag="VFL84_14100"
     CDS             complement(1283..>2027)
                     /locus_tag="VFL84_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017276125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7609808.1"
                     /translation="PCLSQFVLVCFNGTPKLVAVLVQPRSYGGRALEVLVTFLEGGDA
                     DKPEAVDRGESADSLVVSLRDKLRIAEQRLETMRQEHALAYEDLRAANEELQSLNEEY
                     RSTTEELETSKEELQSVNEELQTVNNELKMKLEEVSRANNDLENFMAATDIPMLFLDR
                     GLSIKRYTAPLRQIFNVKNHDQGRPIGDLTHNVLYDDLEKDAAAVLANLSPVERRIKT
                     RDGEVLTVRIRPYRTSEDKIDGVVVTFVK"
BASE COUNT          314 a          699 c          663 g          351 t
ORIGIN      
        1 gacgcacgcg atagccgcga tcttcgtcac gaacgaagaa cgcggccaat cgctcgggcg
       61 acacgagacc ctcgatctcg gcgggataaa aaccgctgcg cgcgcgttgc aacgactcat
      121 cggacatgtc gctggcaaac acctggatgc tgggccgcac gtcgcggcga gcgcgctgct
      181 cgagcagcgc gatggcgagc gagtatgcgt cctcgcccgt cgagcagccg acgacccaag
      241 cgcgaaacgt gtcgcggtcg cgcgtcttcg tcgcgaacag ccgcggcaac acgtcggact
      301 cgagccgctc gaatttcctc tcgtgaccga agaactcggt cacgttcagc aacagctcgt
      361 tggcaagcgc cgcggcttcc gccgtgtccg ttcgcagaag cccgagatac gtgggccagt
      421 cgtcgatcgc gtggattcgc atccgcttgg cgacgcgctg cgcgagcagc gattgccggt
      481 agactgcgaa gtctcggccc gttcgctgct cgagcagcgc gagcagctcg ccaaacagcg
      541 ccgactcgtc cgtgccctgt tcacgccggt cgtgcttgag ctgcgggtcc gtgccgcaat
      601 agcgctcgat ggccgacggg atgtcgcgca gcggcagaac gaggtcgacg acgccggaat
      661 cgagcgcgct gctcggcatg ccggcgtact cggcctcggc cgggtcctgc acgatcgtga
      721 ggccgcctcg atccttgatg ccgccgagac cgagcgagcc gtcggagccg gcgcccgtca
      781 acaagacgcc tacgccgcgg ctgccgcgtc ccgcagcgac ggcgcggaac aagcggtcga
      841 tgcgcccgcg ccgcgcctcc gcggccggca tgtcgacgag ccgcaagtgc gagtcgatga
      901 cgccgacttc ggcgcttgcc ggcacgagga gaatcgtgtg aggttcgagc agctcttcgc
      961 gcgccgcttc gcggaccgga agctccgtgt acggctggag caggccgtcg agccggccgc
     1021 cactgctcga cgagcggtgc acggcgacga cgaacgtggc gctcgagctc gcctcgaggc
     1081 cgccgagcac ggtccggagc gcctcgagcg cgccggccga ggcgccgatg gcgacgacat
     1141 ggccgagctg agccgatgcg ccggcggccg cgctggtcgc ttccctcgcc ttttcgttcg
     1201 ccgccatgtc ggacggtctc ctagctcgac tacgctcttc ccgttcggcg ggtccgtcta
     1261 caaattccgt gccacgccgc tctcatttga cgaaggtcac gacgacgccg tcgatcttgt
     1321 cctcggaggt gcggtacggc ctgatccgca cggtcagcac ctcgccgtcc cgcgtcttga
     1381 tgcgccgctc gactggcgac agattggcga gcacggccgc cgcgtccttc tcgagatcgt
     1441 cgtagaggac gttgtgcgtg agatcgccga tcggccgccc ctgatcgtga ttcttgacgt
     1501 tgaagatctg gcgcagcggc gccgtatacc gcttgatgct caagccgcgg tcgaggaaca
     1561 acatcggaat gtcggtcgcc gccatgaagt tctcgaggtc gttgttggcg cgcgagactt
     1621 cctcgagctt catcttgagc tcgttgttga cggtctgcag ctcctcgttg acgctctgca
     1681 gctcttcctt gctcgtctcg agctcttcgg tcgtcgagcg gtactcctcg ttcaagctct
     1741 gcagctcttc gttggccgcg cgcagatctt cgtaggccaa cgcgtgctct tgccgcatcg
     1801 tctcgagccg ctgctcggcg atgcgcagct tgtcgcgcag gctcacgacg agcgaatccg
     1861 ccgactctcc gcgatccacg gcctccggct tgtccgcgtc gccgccttcg aggaacgtga
     1921 ccagcacttc gagcgcgcgg ccgccgtacg agcggggttg caccagcacg gcgaccagct
     1981 tcggcgtgcc gttgaagcac accagcacga attgagacaa gcacggc
//
LOCUS       DASSNN010000750         3505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1333345, whole genome shotgun sequence.
ACCESSION   DASSNN010000750 DASSNN010000000
VERSION     DASSNN010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333345"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VFL84_14105"
     CDS             complement(<1..490)
                     /locus_tag="VFL84_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7609809.1"
                     /translation="MNVATHVRHATRRDLLRHVAALAGAALLPSRLAAQDARLETVRL
                     SPRMNAILGPDATVLAADSDDGVVLVDGGHASWADALLQTVASLYPAKPVRALFNTHW
                     HEEQTGANRVLGERGVEIVAHENTKLWLGKEVWVRWSDKKYPPLPQRALPTTTVYDPG
                     RMT"
     gene            complement(487..909)
                     /locus_tag="VFL84_14110"
     CDS             complement(487..909)
                     /locus_tag="VFL84_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HET7609810.1"
                     /translation="MLTTSLVPPVLALIGWTFVMWVWLYATRIPALRAARVDLVAVSS
                     TGAKLDLPPEVARVADNYNHLHEQPTIFYALALAAEHGAAVDGISVALAWAYVALRVI
                     HSFVQATRNVIIVRFYVFATASVVLFLLLLRTIVLLVP"
     gene            1043..3343
                     /locus_tag="VFL84_14115"
     CDS             1043..3343
                     /locus_tag="VFL84_14115"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7609811.1"
                     /translation="MAPDLPQRLAAILAADIAGYTRLMELDEAGTIASWRLARAEIID
                     PTIARNGGRIVKLTGDGFLAEFSTAESAVRAALTMQEKLTTRFAAQPADRRVAFRMGI
                     DIGDIWVDEQDVYGTGVNIAARLESLATPGALCISDAVHAAVKHKIEARYEDLGPRRV
                     KNVATAVHVWRASMATPLVARCRPAALAGGTIAVVALIALTAGVVYFGRPPSSPGAAV
                     AGPSGEVAPVEAPPAATVTAPAVDPNTIAVLRLYNIDGSEEMRIFADGLAEDIINRLS
                     ATPPLRVSSRGDSFALDANSSGEAVRSRLRVMYFIEGSVRRSGDTLRVVVKLVDSASG
                     FQIVSRQFDKPIGEFLQLQDEITKLVVANLRVALPSLADEPAYASAETASYDAYLAYR
                     RGMDILGRPLTREAVTEALRAFRQSLAVDADYAAAFAGICLANIAGYDVTQEGAYMIS
                     ADSSCNSALDRNANLIVVHDALGALYLRTGRTDDAERAFERTLAIHRDDVPALTGLGD
                     VYSKQQRLADAEQRYRHAIGLQPGNWRTSNALGNFLFRTGRYEEAAAAYGDVVALDTA
                     NGTGWANLASAAMLSGDFTSAVSAFERALEVEPSAQTYMNLGMLHYYRGEADAAKEAL
                     DKAIAMTPENHVVWSNLGDVLTLVGDVDGANRAFGEANRLARDKLARNSRDATTLLDL
                     AWITAMLGGFAEAQQLIGTAKALDPSDPYVPYYDALVRARMGDTDNALDRLETAVEMG
                     YSRVLIRAEPHLAALHGSSRFDALVK"
BASE COUNT          610 a         1186 c         1163 g          546 t
ORIGIN      
        1 acgtcatgcg gcccgggtcg tacacggtcg tcgtcggcag cgcgcgttgc ggcagcggcg
       61 gatacttctt gtccgaccag cgcacccaga cttccttgcc gagccagagc ttcgtgttct
      121 cgtgcgcgac gatctcgacg ccgcgctccc cgagcactcg attcgcgcct gtctgctctt
      181 cgtgccagtg cgtgttgaac agcgctcgca cgggcttggc cggatacagg ctcgcgacgg
      241 tttgaagcag cgcatcggcc cacgatgcat gcccgccgtc gacgagcacg acgccgtcgt
      301 cgctgtcggc cgcgagcacg gtggcgtcgg gtcccaggat cgcgttcatg cgtggcgaga
      361 gccgcaccgt ctcgagcctc gcgtcctgcg cggcgagccg cgagggaagc agcgccgcgc
      421 cggcgagcgc ggccacgtgc cgcagcaagt cgcggcgtgt cgcgtgacga acgtgcgtcg
      481 ccacgttcat ggaaccaaca gcacgatcgt acgcagcagc agcaagaaca agacgaccga
      541 cgccgtcgcg aagacgtaga agcgcacgat gatgacgttg cgcgtcgctt gcacgaacga
      601 atgaatcacg cgcagcgcga cgtaggccca cgcgagcgca acgctgatcc cgtcgacggc
      661 ggccccgtgc tcggccgcga gcgcgagcgc gtagaagatc gtcggctgct cgtgcaggtg
      721 gttgtagtta tcggcgacgc gcgcgacctc gggcggcaga tcgagcttcg cacccgtgct
      781 cgacacggcc accagatcga ctcgcgcggc acgcagcgcg ggtatgcgag ttgcgtagag
      841 ccagacccac atgacgaacg tccacccgat cagcgcgagg acgggcggaa cgaggctcgt
      901 cgtcagcacg tgcaataacc ctcggatttt cctggggcga gtattacgcg cgggtcggga
      961 ttccgacaag acgacctgcc gccaggcgcc gcggccggtg ctatagtgcc ccgtccacgt
     1021 tgcgcacggc aaagggtcct cagtggcgcc agaccttccg caacgactcg ccgccatcct
     1081 cgcagccgac atcgcgggtt atacgcggtt gatggagctc gacgaggccg ggacgatcgc
     1141 gtcatggcgc ctcgcccgcg ctgaaatcat cgatccgacg attgcacgca acggcggccg
     1201 catcgtcaaa ctcacgggcg acgggttcct cgcggagttc tccacggcgg agagcgccgt
     1261 gcgcgcggcg ctcacgatgc aggaaaagct cacgaccagg ttcgccgcgc agccggccga
     1321 ccggcgcgtt gcgtttcgca tgggaatcga catcggcgac atctgggtcg acgagcaaga
     1381 cgtctacggc accggcgtca acatcgcggc acggctcgaa tcgctcgcga cgccgggcgc
     1441 gctctgcatc tccgacgccg tgcacgcggc cgtcaagcac aagatcgaag ctcgctacga
     1501 agacctcggg ccgcggcgcg tcaagaacgt ggcgactgcc gtgcatgtct ggcgggccag
     1561 catggcgacg ccgcttgtcg cgcgctgcag gccggcggcg ctcgcgggcg gcacgatcgc
     1621 ggtcgtagcg ctgatcgcgc tcacggcagg cgtcgtctac ttcggccgcc cgccgtcgag
     1681 cccaggcgcg gcagtcgccg gcccgagcgg cgaggtcgcg ccggttgaag cgccgccggc
     1741 agccacggtg acggcgccgg ccgtcgatcc caacacgatc gccgtgctgc gcctctacaa
     1801 catcgatggc agcgaggaga tgcgaatctt cgcggacggc ctcgccgaag acatcatcaa
     1861 tcggctctcc gcgacgccgc cgctgcgcgt ttcgtcgcgc ggcgattcgt tcgcgctcga
     1921 cgccaacagc tcgggcgaag ccgtgcgtag tcggctgcgt gtcatgtact tcatcgaagg
     1981 cagcgtgcgc aggagcggcg acacgctgcg cgtcgtggtc aagctcgtcg actccgcgag
     2041 cggctttcag atcgtgtcgc gccagttcga caaaccgatc ggcgagttcc tgcaacttca
     2101 ggacgagatc acgaagctcg tcgtcgcgaa tttacgtgtt gcgctgccgt cgctcgccga
     2161 cgagccggcg tacgcgtcgg cggagacggc gtcgtacgac gcctacctcg cgtatcgccg
     2221 cggcatggac atcctcggcc ggccgttgac gcgcgaggcc gtgaccgaag cgctacgagc
     2281 gttccggcaa tcgcttgccg ttgacgccga ctacgccgcc gcctttgcgg gaatctgtct
     2341 cgcgaacatc gccggctatg acgtgactca ggaaggcgcc tacatgatca gcgcggactc
     2401 gtcgtgcaac tcggcactcg accgcaacgc taacctgatc gtcgttcacg atgcgctcgg
     2461 cgcgctgtac ctgcggacgg gccgcaccga cgacgcggag cgtgccttcg agcgcacgct
     2521 cgccatacat cgcgacgacg tccctgcgct gacgggcctt ggcgacgttt actcgaagca
     2581 acagcggctc gccgacgccg agcaacgcta ccgccacgcc atcgggctac agccgggcaa
     2641 ctggcgcacg tccaatgcgc tcggcaactt cttgttcagg accgggcgtt atgaagaggc
     2701 cgccgcggcg tatggggacg tcgtggcgct cgacacggcc aacggcacgg gctgggcgaa
     2761 cttggcatcg gccgcgatgc tgtcgggcga cttcacgtca gccgtgagcg cattcgagcg
     2821 cgcactcgag gtcgagccga gcgcgcagac ctacatgaac ctcggcatgc tgcattacta
     2881 ccgtggcgag gccgacgccg ccaaagaggc gctcgacaag gccatcgcga tgacgcccga
     2941 gaaccacgtg gtttggtcca acttgggcga cgtgctcacg ctcgtgggcg acgtcgacgg
     3001 cgcgaacaga gcctttggcg aggccaaccg gctcgcgcgc gacaagctcg cgcgcaacag
     3061 ccgcgacgcg acgaccttgc tcgacctcgc ttggatcacc gccatgttgg ggggcttcgc
     3121 cgaggcgcag cagttgatcg gtacggcgaa ggcgctggat ccgagcgacc cctacgtgcc
     3181 ctattacgac gccctcgttc gagcgcgcat gggcgatacc gacaacgcgc ttgatcggct
     3241 ggagacggcg gtcgagatgg gttactcacg cgtgctgatt cgcgcggagc cgcacctcgc
     3301 ggcgctacac ggcagctcgc ggttcgatgc gctcgtgaaa tgaaacaaca accaacaaga
     3361 aggacagtct atgagaagca cgactatacc cgcgggccta ttgctgacgt tgtcgctgac
     3421 gggatgcggc agcgcagtga agctcaccga ttccactgat ccgatgtgtc cccgcttcga
     3481 ccagatcact ttgcaatacg gcgat
//
LOCUS       DASSNN010000751         4644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1876924, whole genome shotgun sequence.
ACCESSION   DASSNN010000751 DASSNN010000000
VERSION     DASSNN010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876924"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="VFL84_14120"
     CDS             <1..1257
                     /locus_tag="VFL84_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7609812.1"
                     /translation="QTLESKIVDTVQEKVENGIVGEITDTVRGVAETAGELVDAAGNA
                     VTDAAAEPARFGPELDPLGRTIEAETWIILVPAEYADRIASWGFTIRRREDLATLNRV
                     LLTVAAPEDRDIAQAALELALDAPGTQVDFNHVYRPGAEPDGDGLAAKPAVPAVAPPH
                     SPAPARSVGIIDTAVATEHEALREADIVQRDFVPFTNERPTAHGTAVASILVGSAARL
                     HAQLRGGRVYAASVFFEDESGDDATTAASLTVAGEWLAAEGVHVINMSLAGPPNRVLE
                     AAIQALVGRGVVVVAAVGNNGPTGEPLYPAAYPNVVGVTAVDAAQRVYRYANRGPHVR
                     FAAPGVDIRVAKSGGGLRDMTGTSIASPYAAAVIAQTLATRTGATPASVVAELERAAV
                     DLGAKDFDSVFGFGLIEPLTPITASR"
     gene            complement(1241..2239)
                     /locus_tag="VFL84_14125"
     CDS             complement(1241..2239)
                     /locus_tag="VFL84_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609813.1"
                     /translation="MTTRLATLVVAASLCAVPAVGSAQGEVTGDGGGASEAAAGRPPS
                     PASGAASGDSADAVSFEVGAGLEYDSNVAVLELDASTGAGDAAASLDFGVAYDRPGNA
                     KLDFGAGYNFSETAHEDFSAFDVRIHRASASTSYDLGRFDVGATLQYADAALDGNEFL
                     ALTQLSPYVSGLVGRRLFLRFAYLDSDKDFADNPARDAAASSFSSDAYVFINGLKTYL
                     VFGHRYDDEDAGLDELDYTGQRFGLQLSQRFTARSREITLKTELRAESRDYRAVTPSI
                     GELRRDDRRQLGLTVEIPAGQRVVMRIAIRHADNESNLASVDFPETVGSIAFSAKL"
     gene            complement(2373..2918)
                     /locus_tag="VFL84_14130"
     CDS             complement(2373..2918)
                     /locus_tag="VFL84_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609814.1"
                     /translation="MRTQMTLVAAAVAFALGFAAEAGAERTTGGVDATARSTTEASVR
                     EQTALELRGARVRGLARADFDARAGAEGDFDSAFDAQHSNETEAGIEAQSRSEIEADV
                     DSELAVEIGTAVAGEAEAAVDAVIAQSIEAEIASSVDAAVAAEVASAVQSEVESAVES
                     EVQAAVDSEIESAVGALVGGP"
     gene            3137..4114
                     /locus_tag="VFL84_14135"
     CDS             3137..4114
                     /locus_tag="VFL84_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609815.1"
                     /translation="MKALALIIATSLGVATAALAQQTSVRGEASAESQTSVSANRNGA
                     NVASDNRAQGGVAVERDRKDGTREQAATGFSGASEMNATLERSVDTRRAKVGDEVTAK
                     TNETFTTASGATIPRGSRLVGRVTEARARERGEAAAASQLGIVFERAELPDGRVVPMQ
                     ATIQAVAAARSQAQGELGSASHGTDAFGSSHASGGGLAGGGLTGGAIGGVGAASGHVV
                     GSAAGGAGGAVRGATRLPRSDVGAASNGAVGATAGGALLGSSAGAVGGLNASGRWLAG
                     SRGVFGIGDLELASAAAGGAQGSLITSPSRNVRLDGGTQLLLVGSAASR"
     gene            4241..>4644
                     /locus_tag="VFL84_14140"
     CDS             4241..>4644
                     /locus_tag="VFL84_14140"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006084144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET7609816.1"
                     /translation="MARHGMVATSQPLATEIGVATLRRGGSAVDAAIAANAALGLTEP
                     TSCGIGGDLFAIVWEAASERLHGLNASGRSPRSLTLERLRDLGHEFIPERGALAVSVP
                     GAVDGWCELHARFGRLPFHDLLAPSIHYAREG"
BASE COUNT          688 a         1648 c         1645 g          663 t
ORIGIN      
        1 caaaccctcg aatcgaagat cgtcgacacc gttcaggaga aggtcgagaa cggcattgtc
       61 ggcgagatca cggacaccgt gcgcggcgtc gccgagacgg ccggagagct cgtcgatgcc
      121 gccggcaacg ccgtcaccga cgccgccgcg gagcccgcgc gcttcgggcc ggagctcgat
      181 ccgctcggcc gcacgatcga agccgagacc tggatcattc tcgtgccggc cgagtacgcc
      241 gaccgcatcg cgagctgggg attcacgatc cgccggcgcg aagacctcgc gacgctgaac
      301 cgcgtgctgc tgacggtcgc ggcgcccgag gatcgcgaca tcgcgcaggc cgcgctcgag
      361 ctcgcgctcg acgcgcccgg cacgcaggtc gacttcaatc acgtctatcg gccgggcgcc
      421 gaaccggacg gcgacggctt ggctgcgaag ccggctgttc ctgccgtcgc gccgccgcat
      481 tcgcccgcgc ccgcgcgctc ggtcgggatc atcgacacgg ccgttgcgac cgagcatgaa
      541 gccctgcgcg aagccgacat cgtgcagcgg gacttcgtcc cgttcacgaa cgagcggccg
      601 acggcgcatg gcacggccgt cgcgtcgatt ctcgtcggga gcgccgcgag gttgcacgcg
      661 cagcttcgcg gcgggcgcgt ctacgccgca tcggtgttct tcgaggacga gagcggcgac
      721 gacgcgacga cggccgcgag cctgaccgtc gccggcgagt ggctcgccgc cgaaggcgtg
      781 cacgtcatca acatgagtct cgccgggccg ccgaaccgtg tcctcgaggc cgctattcaa
      841 gcgctcgtcg ggcgcggcgt cgtcgtcgtc gcggccgtcg gcaacaacgg cccgacgggc
      901 gagccgctct acccggccgc gtatccgaac gtcgtcggcg tgacggcggt cgacgcagcg
      961 cagcgcgtct atcgctacgc gaaccgcggg ccgcacgtgc ggttcgcggc gccgggcgtc
     1021 gacatccgcg tcgcgaagag cggcgggggc ctaagggaca tgaccggcac ctcgattgcg
     1081 tcgccgtacg cggcggccgt catcgcgcaa acgctcgcga cgcgcacggg cgcgactccc
     1141 gcttccgtcg tcgccgagct cgagcgcgcg gccgtcgatc tcggcgcgaa ggatttcgac
     1201 agcgtgttcg gtttcggtct catcgagccg ctgacgccga tcacagcttc gcgctgaacg
     1261 cgatcgagcc gaccgtctcc gggaaatcga cgctcgcgag attcgattcg ttgtccgcat
     1321 gccggatggc gatgcgcatg acgacgcgct gcccggcggg aatctccacg gttaggccaa
     1381 gctggcgccg atcgtcgcgg cgcagctcgc cgatcgacgg cgtgaccgcg cgatagtcgc
     1441 ggctctcagc acgcagctcc gtcttcagcg tgatctcgcg cgagcgcgcc gtgaagcgtt
     1501 gcgagagctg gaggccgaag cgctgccccg tgtaatcgag ctcgtccaag ccggcgtcct
     1561 cgtcgtcgta acggtggccg aacacgaggt aggtcttgag accgttgatg aacacgtagg
     1621 cgtcgctcga gaacgagctc gccgcggcgt cgcgcgccgg gttatcggcg aaatccttgt
     1681 ctgaatccaa gtacgcgaac cgcaggaaca gccgccgccc gacgagcccc gacacgtacg
     1741 gcgagagctg cgtcagcgca aggaactcgt tgccgtcgag ggccgcgtcg gcgtactgaa
     1801 gcgtggcgcc gacgtcgaag cgcccgagat cgtagctcgt gctcgccgag gcgcggtgga
     1861 tgcggacgtc gaacgccgag aagtcctcgt gcgccgtctc ggaaaagttg tagcccgcgc
     1921 cgaagtcgag cttcgcattg ccgggccgat cgtacgcaac gccgaaatcg agcgacgccg
     1981 cggcgtctcc ggcgcccgtc gacgcgtcga gctcgagcac ggcgacgttg ctgtcgtatt
     2041 cgagtccggc gccgacctcg aacgaaacgg cgtcggccga gtcgcccgag gcggcgccgc
     2101 tcgcgggcga ggggggccgc ccggcggccg cttcgctcgc accgccgcca tcgccggtga
     2161 cctccccttg cgcggagccg acggcgggaa cggcgcagag cgaagcagcc acaaccagag
     2221 tcgcgagtcg tgtcgtcatt tatttgcgcc tcgcacgtat tgtccggaat tcgcgcccac
     2281 aagcgacctg tcgtcgaacg gagtcaccgt ttccaaagat gagaaaagag agatccgggc
     2341 cggcaatcac tcgacggccg gcccggagcc catcatgggc cgccgacgag ggcgccgact
     2401 gccgactcga tctcgctgtc gacggcggct tgcacctccg attcgacggc gctttcgacc
     2461 tcggactgga cggcgctcgc gacctcggcc gcgaccgccg cgtcgaccga agacgcgatc
     2521 tccgcctcga tggactgcgc gatgaccgcg tcgacggccg cctcggcctc gccggcaacc
     2581 gccgttccga tctcgacggc cagctccgaa tcgacgtccg cttcgatctc ggatcggctc
     2641 tgcgcttcga tcccggcctc cgtctcgttc gagtgctgcg cgtcgaaggc gctgtcgaaa
     2701 tcgccttcgg cgcccgcacg cgcgtcgaaa tccgcgcgag caaggccgcg cacgcgcgcg
     2761 ccgcggagct cgagcgcggt ttgctcgcgc acgctcgctt ccgtggtcga acgcgccgtc
     2821 gcgtcgacgc cgccggtcgt ccgttcggcg ccggcttcgg cggcgaaacc gagcgcgaac
     2881 gcgacggcag ccgccacaag agtcatctga gtacgcatgt cgtaatcctc cctgaggttg
     2941 gtcctcgcta agagaacggg cgtcgcggcg catctattcc cgccgcgagt cgcgttatgt
     3001 cacgaagcga gcattggcgc ggcgcctcgg gcaacgggaa taagtcgacg gctcggccgt
     3061 tatcggtatg tccacgcgca tcgagcgcgc tcaacggcgc agagggacgc gccgcatcga
     3121 tagaggagga atcgcaatga aagctctagc tctgatcatc gctacttcgc tcggcgtcgc
     3181 cacggccgcg ctcgcgcagc aaaccagcgt tcgtggcgaa gcgtccgccg agtcgcagac
     3241 ttccgtctcc gccaacagaa acggcgcgaa cgtcgcgagc gacaaccgcg cgcaaggcgg
     3301 cgtcgccgtc gagcgcgatc gtaaggacgg cacgcgcgag caggcagcga ccggcttctc
     3361 gggcgcgtcg gagatgaacg cgacgctcga gcgctccgtc gacacgcgcc gcgcgaaggt
     3421 gggcgacgag gtcacggcca agacgaacga gaccttcacg acggcgagcg gcgcgacgat
     3481 tccgcgcggc tcacggctcg tcggtcgcgt gaccgaggcc cgggcccgcg agcgcggcga
     3541 ggctgcggcc gcgtcgcagc tcggcattgt gttcgagcgg gccgagcttc cggacggccg
     3601 cgtcgtgccg atgcaggcga cgatccaggc cgtcgcggcc gctcggtcgc aggcgcaggg
     3661 cgagctcggc tccgcgtcgc acggcacgga cgccttcggc tcctcgcacg cctcgggcgg
     3721 cgggttggcc ggcggcggcc tgacgggcgg cgcgatcggc ggcgtcgggg ccgcaagcgg
     3781 ccacgtcgtc ggcagcgcgg ccggcggggc cggcggagcg gtccgcggtg cgacgcgctt
     3841 gccgcgctcc gacgtcggcg cggcgtcgaa cggcgcggtc ggcgccacgg ctggcggcgc
     3901 gctgcttggc tcgtcggccg gcgcagtggg cggcttgaat gcctccggcc ggtggctcgc
     3961 cggcagccgc ggcgtgttcg gcatcggcga tttggagctg gcgtcggccg cggccggcgg
     4021 cgcgcagggg tcgctgatca cctcgccgtc gcgcaacgtc cggctcgacg gcggcacgca
     4081 gctgttgctc gtcggcagtg cggcgagccg ctgacgcttg ctcgagaccg acgagtcgag
     4141 gggttgccgg cgacggcaac ccctctttcg ttcgcctgcc agaccgccac tacaatgccg
     4201 cgtggaccga ctcgaccgct tcgcaacccg ctccgtcgtc atggcccgcc acggcatggt
     4261 cgcgacgagc cagccgctcg cgaccgagat cggcgtcgcg acgctgcgcc gcggcggcag
     4321 cgccgtcgac gcggccatcg ccgcgaatgc cgcgctcggc ctcacggagc cgacgagctg
     4381 cggcatcggc ggcgacctgt tcgcgatcgt atgggaagcc gcgagcgaac ggctgcacgg
     4441 attgaacgcg agcggccgct cgccgcgctc gctgacgctc gagcggttgc gcgatctggg
     4501 ccacgaattc atccccgagc gcggcgcgct cgccgtcagc gtaccgggcg ccgtcgacgg
     4561 ctggtgcgag ctgcacgcgc gcttcgggcg cttaccgttc cacgacctgc tcgcgccgtc
     4621 gattcattac gcccgcgagg gttt
//
LOCUS       DASSNN010000752         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_363401, whole genome shotgun sequence.
ACCESSION   DASSNN010000752 DASSNN010000000
VERSION     DASSNN010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363401"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFL84_14145"
     CDS             <1..546
                     /locus_tag="VFL84_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609817.1"
                     /translation="PEHVRGLPILAADSTANVGTALDLLARGARQVLLLAAPEPRIER
                     LLIYVEPSSFGRDSVLAAAASLARHLPIDVGMLVRDEEDDGYRDLLDLRNLSLRQHGL
                     DIRTETFRGNATDAVLERLVTSDAQTLLVIGLSAPERCSDLVDDLQKLLREQPPAAVL
                     FVSGRDRTQAQSQPHAAAFSL"
     gene            564..1643
                     /locus_tag="VFL84_14150"
     CDS             564..1643
                     /locus_tag="VFL84_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7609818.1"
                     /translation="MRGFLFAFVVLLTACGGGDQDRRDGELRLLNVSYDPTRELYREF
                     NDQFIAKWLADTGQRVSVQMSHGGSGSQARAVIDGLNADVVTLALAWDIDNIQSLSRR
                     LPASWQSRLPNNSSPYTSTIVFLVRKGNPKGIHDWPDLVNGDVQIITPNPKTSGGARW
                     NYLAAWGFALDRALGGNIDELKSLPADKVAAAEAEAEKFVGELYRRVPVQDTGARAAT
                     NTFVQRKIGDVLLAWENEALLAQREMGSDAFDIVWPSVSVLAEPPVALIDSVVDRKGT
                     RAVAEAYLDYLYAPEGQDIIARNFFRPTNAEVAARYTEQFPEIRTFTVDAVFGGWARA
                     QAEHFADGGIFDRLFLAASGAPYRP"
     gene            1682..2527
                     /gene="cysT"
                     /locus_tag="VFL84_14155"
     CDS             1682..2527
                     /gene="cysT"
                     /locus_tag="VFL84_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HET7609819.1"
                     /translation="MATARGWVSPSVLPGFGLTLGFAGLFLSFIVLIPMAALIIRAGS
                     LPLDEFWRIATEPRALASYRLTFGASFLAACVNAVFGFIIAWTLVRYEFPGRRIVDAI
                     IDLPFALPTAVSGIALATVFAPNGWLGRHLESIGVQVAYTWVGVVVALTLIGLPFVVR
                     SVQPALEEVDPSLEEAAESLGASRWMVFRRVILPTVLPALATGFTLALARAVGEYGSV
                     IFIAGNLPMRTEITPLLIVIKLEQYDYLGAAALGLVMLCVSFVMLLSINAINGWRIRR
                     MAGRR"
     gene            2527..>3160
                     /locus_tag="VFL84_14160"
     CDS             2527..>3160
                     /locus_tag="VFL84_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7609820.1"
                     /translation="MAAAAHSVRRQHSPFVRAALIAVSVLFLAAILIMPVVSVLGYAF
                     SRGLAVYLEALHAPDTLAAIRLTLTTAAIVVPTNTIFGVAAAWAIAKFDFRGKQILIT
                     LIDLPFSVSPVIAGLVFVLLFGAQGWFGPFLRDQGIQVVFALPGIVLATMLVTFPYVA
                     RELIPIMQLQGREAEEAAVSLGAGGWMTFFRVTLPSIRWGLLYGVILCNAR"
BASE COUNT          441 a         1076 c         1059 g          584 t
ORIGIN      
        1 cccgagcacg tccggggctt gccgatcttg gcggccgact cgacggcaaa cgtcgggacg
       61 gcgctcgacc tgctcgcgcg cggcgcgcgc caggttctcc tgctcgcggc gccggagccg
      121 cgcatcgagc gactgctgat ctatgtcgag ccgtcctcgt tcggccgcga cagcgtgctg
      181 gcggccgcgg cgagcttggc ccgtcatttg ccgatcgacg tcggcatgct cgtgcgcgac
      241 gaggaagacg acggctatcg cgacctgctc gatcttcgca acctctcgtt gcgccagcac
      301 ggcctcgaca tccgcacgga gacgttccgc ggcaacgcga cggacgccgt cctcgagcgt
      361 ctcgtgacgt cggacgcgca gacgctgctc gtgatcggct tgagcgcgcc cgagcgatgc
      421 agcgatctcg tcgacgatct gcagaagctg ctgcgcgagc agccgccggc cgcggttctg
      481 ttcgtcagcg gccgcgaccg gacgcaggcg caatcgcagc cgcacgcagc ggcgttctcg
      541 ttgtgagcgc aaggatggcc ctcatgcgtg gcttcttatt cgcgttcgtc gtgctgctca
      601 cggcttgcgg cggaggcgac caggaccggc gcgacggcga gctgcggctc ttgaacgtct
      661 cgtacgatcc gacccgggag ctctatcgcg aattcaacga ccaattcatc gcgaaatggc
      721 tggccgacac cgggcagcgc gtttccgtgc aaatgtcgca cggcggctcg ggcagccaag
      781 cgcgggccgt catcgacggc ctgaatgccg acgtagtgac gctcgcgcta gcgtgggata
      841 tcgacaacat ccagtccctg agccgcaggc tgccggcgtc gtggcagtcg cgcttgccga
      901 acaacagctc gccatacacg tcgacgattg tttttctcgt gcgcaagggc aatccgaagg
      961 ggattcacga ctggccggac ctcgtgaacg gcgacgtgca gatcatcacg ccgaacccca
     1021 agacgtcggg cggcgcgcgc tggaactacc tcgcggcgtg gggcttcgcg ctcgaccgcg
     1081 cgctcggcgg caacatcgac gagctcaagt cgctgcccgc ggacaaagtt gcagcggccg
     1141 aggccgaggc cgagaaattc gtcggcgagc tctatcggcg cgttcccgtg caggatacgg
     1201 gcgcccgcgc ggcaacgaac acgttcgtgc agcgcaagat cggcgacgtg ctgctcgcgt
     1261 gggagaacga ggcgctgctc gcgcagcgcg agatgggctc ggacgcgttc gacatcgtct
     1321 ggccgtccgt cagcgtcctc gccgagccgc ccgtggcgtt gatcgattcg gtcgtagacc
     1381 gcaagggcac gcgcgccgtc gccgaggcgt atctcgatta cctctacgcg cccgagggcc
     1441 aggacatcat cgcgcgcaat ttcttccgcc cgacgaacgc cgaggtcgcc gcccggtata
     1501 ccgagcagtt tcccgagatc cgcacgttca cggtcgatgc cgtgttcggc ggctgggcgc
     1561 gcgcgcaagc cgagcacttc gccgacggcg gaatcttcga tcggctgttc ttggctgcat
     1621 ccggcgcacc gtatcggcca tgagcgcgcg cgccaccgat acggacgtcg gaacttaagc
     1681 catggcgacc gcgcgaggct gggtcagtcc cagcgtgctg ccggggttcg gtctgacgct
     1741 cggtttcgcc gggctgttcc tctcgttcat cgtgctgatc ccgatggcgg cgctgatcat
     1801 ccgggccggc agcctgccgc tcgacgaatt ctggcgcatc gccacggagc cgcgcgcgct
     1861 cgcgtcgtac cggctcacgt tcggcgcctc gttcctcgcg gcgtgcgtga atgcggtctt
     1921 cggcttcatc atcgcctgga cgctcgtccg ctatgagttt ccgggccggc gcattgtcga
     1981 cgcgatcatc gacctgccgt tcgctctgcc gaccgccgtg tcgggcatcg cgctcgcgac
     2041 ggtgttcgcg ccgaacgggt ggctcggcag gcatctcgag agcatcggcg tccaagtcgc
     2101 gtacacgtgg gtgggcgtcg tcgtcgcgct gacgctgatc gggctgccgt tcgtcgtgcg
     2161 gtcggtgcag cccgcgctcg aggaagtcga cccgagcctc gaggaggccg ccgagtcgct
     2221 cggcgcgagc cgctggatgg tcttccgccg cgtgatcctg ccgacggtgc tgcccgcgct
     2281 cgcgaccgga ttcacgctcg cgctcgcgcg tgccgtcggc gaatacggct ccgtgatctt
     2341 catcgccggc aacttgccga tgcgcaccga gatcacgccg ctcttgatcg tcatcaagct
     2401 cgagcaatac gactacctcg gcgcggcggc gctcggcctg gtcatgctgt gcgtgtcgtt
     2461 cgtcatgctg ttgtcgatca acgcgatcaa cggctggcgc atccggcgca tggcgggaag
     2521 gcgctgatgg ccgccgccgc acactccgtt cgcaggcagc acagcccatt cgtgcgcgcg
     2581 gcgctgatcg ccgtgtccgt gctattcctc gccgcgatct tgatcatgcc cgtcgtctcg
     2641 gtgctcggct acgcgttctc gcgcggcctc gcggtttatc tcgaggcgtt gcatgcgccc
     2701 gacacgctcg cggcgattcg tctgaccttg acgacggcgg cgatcgtcgt tccgacgaac
     2761 acgatcttcg gcgtcgcggc ggcttgggct atcgcaaagt tcgacttccg cggcaagcag
     2821 atcttgatca cgttgatcga cctgccgttc tcggtttcgc ccgtcatcgc gggcctcgtg
     2881 ttcgtgctgt tgttcggcgc gcaaggctgg ttcgggccgt tccttcgcga tcagggcatc
     2941 caagtcgtgt tcgcgctgcc cggcatcgtg ctcgcgacga tgctcgtgac gttcccatac
     3001 gtcgcgcgcg agctgatccc gatcatgcag ctgcaaggcc gcgaggccga ggaggccgcg
     3061 gtcagcctcg gcgccggcgg ctggatgact ttcttccggg tgacgctgcc gagcattcgc
     3121 tgggggttgc tctatggcgt catcctctgc aacgcgcgcg
//
LOCUS       DASSNN010000753         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1877270, whole genome shotgun sequence.
ACCESSION   DASSNN010000753 DASSNN010000000
VERSION     DASSNN010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877270"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..>2083)
                     /gene="mrdA"
                     /locus_tag="VFL84_14165"
     CDS             complement(233..>2083)
                     /gene="mrdA"
                     /locus_tag="VFL84_14165"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET7609821.1"
                     /translation="IAAAIIVLLLSGVLVTRLVQLQILNYERFAELSQGNRFRIDPLP
                     PTRGIIYDRNGIVIAENRPNWELVATAEQIDNLDRTLRSLEVLGLVDPAEHGALRTLV
                     RSHRGFERVKLSNLTEEQAATFAVRRHLFSGIDIQEALSRYYPFGDQTAHAIGYVSSI
                     SEQDLQRIDRGNYAGTSHIGKTGIERAYEDRLHGQVGYRQLMVNAQGRVIEAAGAGGK
                     GSTDTGGFESKAPQPGENLVLTLDMKLQLAAQEALGDSRGAVVAIDPRNGDVLALVST
                     PSFDPNRFAAGMSRRDYVALNTDPDKPLFNRALAGTYPPGSTVKPFIALTALQHESVA
                     PEHEIYCPGEWRLPGQTHRYREGRGGVHGSVGLHEAIVRSCDVYFYQLAAAMGIDSLS
                     SGLTAFGFGAPTGLDISGEQSGIVPSPEWKKRNFSRREDQIWFPGETVITGIGQGYVL
                     VTPIQLASAAATFAARGTRFAPRLLLGTENAVTGEMSVLEPRALPSVDDHAPEHWQFV
                     ADAMVGVTSEEGGTARTQMKGLAPHVAGKTGTAQVITIGQDERYREEEIEETQRDHGL
                     FVAFAPAEAPRIALGIVVENGGGGARAAAPVARKVLDAFFAAENYVASQP"
BASE COUNT          331 a          718 c          704 g          330 t
ORIGIN      
        1 cgtgaacagg cgcagatagt gcggcgggat ctgcgccatc acgaagaagg cgccgagacc
       61 gacggcgaac cgcatggctt ggcgaagcag caagctcaag tcggcgtcga cggcgctgta
      121 gagcacgacc atcccgagcc cgactacgcc caatagaatc aggagcaggg gcatgtcgac
      181 gacgaagccc ttgaacccgc gcttgcggcc gccggcggcg gccctgctcc actcaaggct
      241 ggctagcgac atagttttcc gctgcgaaga aagcgtcgag aaccttgcgc gcgaccggtg
      301 ccgcggcgcg cgcgccgccg ccgccgttct cgacgacgat gccgagcgcg atgcgcggcg
      361 cctcggccgg cgcgaacgca acgaacaatc catgatcgcg ctgcgtctcc tcgatctcct
      421 cctcgcgata cctctcgtcc tggccgatcg tgatgacctg ggcggtgccg gtcttgccgg
      481 cgacgtgggg cgcgaggccc ttcatctgcg tgcgcgcggt gccgccctcc tccgacgtca
      541 cgccgaccat cgcatcggcg acgaactgcc aatgctctgg cgcgtggtcg tcgacgctcg
      601 gcaacgcgcg cggctcgagc acgctcatct cgcccgtcac ggcgttctcg gtgccgagca
      661 gcaggcgcgg cgcgaaccgc gtgcctcgcg ccgcgaacgt cgcggccgcg ctcgcgagct
      721 gaatcggcgt cacgagcacg tagccctggc cgatgcccgt gatgacggtc tcgccgggga
      781 accagatctg atcctcgcgg cgcgagaaat tgcgcttctt ccactccggc gacggcacga
      841 tgccgctctg ctcgcccgag atgtcgaggc ccgtcggcgc gccgaagccg aacgccgtga
      901 gcccgctcga caggctgtcg atgcccatcg cagccgcgag ctgatagaag tacacgtcgc
      961 acgagcgcac gatcgcctcg tgcaggccca cgctgccgtg gacgccgccg cggccctcgc
     1021 ggtaacggtg cgtctggccc ggaagccgcc attcgccggg gcagtagatc tcgtgctcgg
     1081 gggccacgga ttcgtgctgc agcgccgtca acgcaatgaa cggcttcacg gtcgagcccg
     1141 gcggataagt gccggcgagc gcgcgattga agagcggctt gtcgggatcc gtgttcaacg
     1201 cgacgtagtc gcggcgactc attccggccg cgaaccgatt cggatcgaac gacggcgtgc
     1261 tcacgagcgc gagcacgtcg ccgttgcgcg gatcgatcgc gacgacggcg ccgcgcgagt
     1321 cgccgagcgc ttcctgcgcc gcgagctgca gcttcatgtc gagcgtcagc acgaggttct
     1381 cgccgggctg aggcgccttc gactcgaacc cgcccgtgtc ggtgctgccc ttgccccccg
     1441 ctcctgcggc ctcgatcacg cggccctgcg cgttcaccat gagctggcgg tagccgacct
     1501 gaccgtgcag ccgatcctcg tacgcgcgct cgattccggt cttgccgatg tgcgacgtgc
     1561 cggcgtagtt gccgcggtcg atgcgctgca gatcctgctc gctgatgctg ctgacgtagc
     1621 cgatcgcgtg cgcggtttgg tcgccgaacg gatagtagcg tgacaatgcc tcttggatgt
     1681 cgatgcccga gaagagatgc cggcgcacgg cgaaagtcgc cgcctgctcc tcggtgaggt
     1741 tgctgagctt cacgcgctcg aagccgcgat gcgagcgcac cagagtgcgc agcgcgccgt
     1801 gctcggccgg atcgacgagc ccgagcacct cgagcgagcg cagcgtgcgg tcgagattgt
     1861 cgatctgctc ggccgttgca acgagctccc agttcggccg attctcggcg atcacgatgc
     1921 cgttgcggtc gtagatgatg ccgcgcgtcg gcggcagcgg gtcgatgcgg aagcgattgc
     1981 cttgcgacaa ctcggcgaac cgctcgtagt tcaaaatctg cagctgaacg agccgcgtaa
     2041 cgagcacgcc ggacagcagc agcacgatga tcgcggccgc gat
//
LOCUS       DASSNN010000754         3871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1259812, whole genome shotgun sequence.
ACCESSION   DASSNN010000754 DASSNN010000000
VERSION     DASSNN010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259812"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VFL84_14170"
     CDS             <1..647
                     /locus_tag="VFL84_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609822.1"
                     /translation="SVARGTTICADAERHCASLVARVSALKQRQQPVLLATRTVAASE
                     RLSGALTRARLQHQVLNARNEPVEAAIVAQAGEAGRVTIATNMAGRGTDIKLGPGVAL
                     LGGLHVIVAECNEAARLDRQMFGRAGRQGDPGSYERVLALDDDVLRAHLPAWLRAWLA
                     GLVGAFPRLGGSLAECVFRAVRARLERRAARQRRLALLEDGRIGDALSFSGSME"
     gene            652..1584
                     /locus_tag="VFL84_14175"
     CDS             652..1584
                     /locus_tag="VFL84_14175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7609823.1"
                     /translation="MIRVMKVSILGLLAACAMPLRAQQAVPLDCLLEPNRVIDVSSAV
                     RGVLSAVDVERGDLVEQDQVVARLDSSVEQAAMELAQARARANAEVQADQINAEFAER
                     RSDRFVTLYDRSAVSTDQLDESKTIARLRELELKQSRENQHLAQLEARQAEEILRRHV
                     IRSPVRGVVVQRYLSPGESAEDRPILRIAEIETLRAEAIIPVAQFGTIQVGQRAIVVP
                     EEPLTGHYVGTVSVVDRVADAASGTFRARVLLDNSDYALPSGLRCAVRFLQPGESVAD
                     NIPSPPASRPLSRAALQSSRPRDLAAAPPSTTAR"
     gene            1596..>3871
                     /locus_tag="VFL84_14180"
     CDS             1596..>3871
                     /locus_tag="VFL84_14180"
                     /inference="COORDINATES: protein motif:HMM:NF025611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HET7609824.1"
                     /translation="MFERLRNRVLSRGSRRDGGRGPAKRLIVEELEPWILMSADAAPA
                     FDAFRSPLTTEHETHELLLDSGELSGSLAVGTVAAGVRHEIVFVDARLADHEQIVSGL
                     PGGSGDRQIEVFVIEAGEDGLERITEVLGARQGVDAVHVFSHGVDGAVELGDSWLNGA
                     VLEMRSETVAGWRSALSADADLLLYGCNVGHDTEGKAFLDRLSELTGADVAASDDATG
                     AAALGGDWNLEVATGSIETSAALGAGVQQSWTHVMAISVGNSSSAASAGAASSLTWAH
                     TVDGGSDGLLLVTVSVETDTGTTVGSVTYGGVALTRLDAATSTRDHVEMWYLKLPAAG
                     TANIVVTLSSGADRVAAGATDFFGVEQDSSAYSSGSVVTASGTGDPSLTIASAAGELV
                     VDGVADRDIDSEFVGASQTVLFTNKTGTSPGDAWVGSSYEAGAASVTMSWTTNGPGGQ
                     WAAIAVALKPAGNAAPVLDASRTPVLSAVAEDAGAPSGAVGTGVQFGLVDFVVPSGQI
                     DSVTDTDPGAVTGVAVIGADTTNGTWWYSTNNGGTWLALGAVSNTNARLLAADGSTRI
                     YFQPNANFNGTIANAITFRAWDRTSGVNGGTANASINGGTTAFSTATDTANLTVTAVN
                     DAPTTAPVTLAPLAEDGGSRLITQAQLLANASDVDGNPLTATALAIASGAGSLVDNGN
                     GTWTYTPAPNDSTSVSFSYTIADGNGGTVAGSATLDLTPVNDPPTTSPVTLAPLAEDG
                     GSRLITQAQLLANASDVDG"
BASE COUNT          582 a         1328 c         1365 g          596 t
ORIGIN      
        1 gcagcgttgc tcgcggcact acgatctgcg ccgacgccga gcggcactgc gcctcgctgg
       61 tcgcgcgcgt gagcgcgctc aagcaacggc agcagcccgt gctgctcgcg acgcgcacgg
      121 tcgccgcctc ggagcggctc agcggcgcgc tgacgcgcgc acggttgcag caccaggttt
      181 tgaacgcgcg caacgagcct gtcgaggccg cgatcgtcgc gcaagccggc gaggccggcc
      241 gcgtcacgat cgcgacgaac atggccggcc gcggcaccga catcaagctc ggccctggcg
      301 tcgcgctgct tggcgggctg cacgtcatcg tcgcggaatg taacgaggcc gcgcgcctgg
      361 atcggcagat gttcggacgc gccggccgcc agggcgatcc gggcagctac gagcgagtgc
      421 tcgcgctcga cgacgacgtc ctgcgcgccc acttgccggc gtggctgcgc gcgtggctcg
      481 ccgggctcgt cggcgctttt ccgcggctcg gcggctcgct ggccgagtgc gttttccgag
      541 ccgtccgcgc gcgcctcgag cgccgcgcgg cgcggcagcg gcggttggct cttttggaag
      601 acggacgcat cggcgatgcg ctgtccttct ccggttcgat ggagtgacga aatgatccgt
      661 gtaatgaagg tgtcgatcct tgggttgctc gcggcatgcg ccatgccgct gcgggcgcag
      721 caggccgttc cgctcgactg cctgctcgag ccgaatcgcg tcatcgacgt gagcagcgcc
      781 gtgcgcggcg tgctctcggc cgtcgacgtc gagcgcggcg atctcgtcga gcaggaccag
      841 gtcgtcgcga ggctcgactc gtcggtcgag caggccgcga tggagctcgc gcaggcgcgg
      901 gcgcgcgcga acgccgaagt gcaggccgac cagatcaacg ccgaattcgc cgagcggcgc
      961 agcgaccgct tcgtcacgct gtacgaccgc agcgcggtct cgaccgacca gctcgacgag
     1021 tcgaagacga tcgcgcggct gcgcgagctc gagctcaagc agtcgcgcga gaatcaacac
     1081 ctcgcgcagc tcgaggcgcg gcaggccgag gagatcctgc ggcgccacgt catccgcagc
     1141 cccgtccgcg gcgtcgtcgt gcagcgctat ttgtcgccgg gcgagtcggc ggaggatcgg
     1201 ccgatcctgc gcatcgcgga gatcgagacg ctgcgcgccg aagcgatcat ccccgtggcg
     1261 cagttcggga ccatccaggt cgggcaacga gccatcgtgg tgcccgagga gccgctgacg
     1321 ggtcactacg tcggcacggt gtcggtcgtc gaccgcgtcg cggacgccgc gagcggcacg
     1381 ttccgcgcgc gcgtgttgct cgacaactcc gactacgcgc tgccgagcgg gctgcgctgc
     1441 gccgtgcgct tcttgcagcc cggggaatcc gtggccgaca acattccgtc gccgcccgcg
     1501 agccggcccc tgtcgcgcgc cgcgctgcag tcctcgcggc cgcgcgatct cgccgccgcg
     1561 ccgccctcga cgacggctcg ctgaggtaac cgcaagtgtt cgagcgcctt cgcaatcggg
     1621 tgttgtcgcg cggctctcgc cgcgacggcg ggcgtgggcc cgcgaagcgc ctgatcgtcg
     1681 aggagctcga gccgtggatc ctgatgtcgg cggacgccgc accggcgttc gatgctttcc
     1741 gctcgccgct tacgaccgag cacgagacgc acgagctgtt gctcgattcc ggcgagcttt
     1801 cggggtccct cgccgtaggc accgtcgccg ccggcgtccg gcacgaaatc gttttcgtcg
     1861 acgcgcggct tgcggaccat gagcagatcg tatccggact tccgggcgga agcggcgatc
     1921 gccagatcga ggtgttcgtc atcgaggcgg gagaggacgg actcgagcgc atcaccgaag
     1981 ttttgggagc gcggcagggc gtcgacgccg tgcatgtctt ttcgcacggt gtcgatggcg
     2041 ccgtggagct cggcgattcg tggctaaacg gtgccgtttt ggaaatgcgc tcggagaccg
     2101 tcgcgggctg gcgaagcgcg ctgtccgccg atgcggacct gctgctgtac ggttgcaacg
     2161 tcggtcacga cacggagggc aaggccttcc tcgaccgtct cagcgagctg acgggggccg
     2221 acgtcgcggc cagcgacgat gcgacgggcg ccgccgctct cggtggcgac tggaacctcg
     2281 aagtcgccac tggatcgatc gagacctcgg cggcgctcgg cgcgggcgtt caacagagtt
     2341 ggacgcatgt gatggccatc agcgtcggca acagctcgtc cgctgcctcc gcgggcgccg
     2401 cctcgtcgct cacgtgggcg cacacagtgg atggcggcag cgacggcctc ctgctcgtta
     2461 ccgtgtccgt cgaaacggac acgggcacca cggtcggctc cgtcacctac ggtggcgtgg
     2521 ctctcacgag gctcgatgcg gccacgtcga ccagagacca cgtcgagatg tggtacctga
     2581 agctgcccgc tgccggcacg gcgaatatcg tcgttaccct gagcagcggc gctgacaggg
     2641 ttgcggccgg cgccacggac tttttcggcg tcgagcagga ctcttctgcc tactcgtccg
     2701 gatcggtggt cactgctagt ggcaccgggg atccgtcgct gacgatcgcg tccgcggcgg
     2761 gcgagctcgt cgtcgacggt gtcgcagatc gcgacatcga ctcggaattc gtcggcgcca
     2821 gccaaaccgt cctgttcacg aacaaaacgg ggacaagccc cggtgacgct tgggtcggca
     2881 gcagctacga ggccggcgcc gcctcggtca ccatgtcctg gaccaccaac ggtccgggcg
     2941 gtcagtgggc cgcgatcgcc gtagccttga agcccgccgg caacgcggcg cccgtgctgg
     3001 atgcgagccg tactccggtc ttgagcgcgg tggccgaaga tgccggcgcg ccgtcgggcg
     3061 ccgtcggcac cggagttcaa ttcgggctcg tagatttcgt agttccgagc ggtcagatcg
     3121 acagcgtcac ggataccgat ccgggcgccg taacgggcgt tgccgtgatc ggcgccgata
     3181 ccacgaatgg cacgtggtgg tattcgacga acaatggcgg cacctggctc gcgctcggcg
     3241 ccgtcagcaa caccaacgca cggttgctcg cggccgacgg gtcgacgcgg atctacttcc
     3301 aaccgaacgc aaacttcaac ggcacgatcg cgaacgccat cacgttccgc gcctgggatc
     3361 gcacgagcgg agtgaacggc ggcacggcga acgcctccat caacggcggc acgaccgcgt
     3421 tctcgacggc gaccgacacg gcgaatctca cggtcaccgc ggtgaacgac gcgccgacga
     3481 cggcgccggt gacgctcgcg ccgctcgccg aggacggcgg gtcgcggttg atcacacagg
     3541 cgcagctcct cgcgaatgct tcggatgtcg acggcaatcc actgacggcg acggcgcttg
     3601 cgatcgcgag cggcgccggc tcgctcgtcg acaacggcaa cgggacgtgg acctacacgc
     3661 cggcaccgaa cgattcgacc tcggtcagct tcagctacac gatcgccgac ggcaacggcg
     3721 gcacggtcgc gggcagcgcg acgctcgatc tcacgcccgt caacgatccg ccgacgacgt
     3781 cgccggtcac tctggcgccg ctcgccgagg acggcgggtc gcggttgatc acacaggcgc
     3841 agctcctcgc gaatgcttcg gatgtcgacg g
//
LOCUS       DASSNN010000755         3848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_992963, whole genome shotgun sequence.
ACCESSION   DASSNN010000755 DASSNN010000000
VERSION     DASSNN010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992963"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1764
                     /gene="speA"
                     /locus_tag="VFL84_14185"
     CDS             <1..1764
                     /gene="speA"
                     /locus_tag="VFL84_14185"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HET7609825.1"
                     /translation="VLLRFQDVLRAQVRRVNEAFRSAIAESNYGNVYRGIYPIKVNQL
                     HEVVDELLDAGRPYGMGLECGSKAELIACLPHIDGETLLVCNGVKDRTMLSLMIAGQK
                     LGQNIVPVMEKYSEFTDVKGLAFSTGYMSQLGVRVRLATRGAGRWAESSGTNSKFGLN
                     VSELMRMVNELEASGQQSKLVLLHCHIGSQIADIQVLKEATKEVAQIYAQLVKRGIAL
                     KYLDVGGGLGVNYDKGHLEEDAGINYALEEYANAVVFTVKEVCDANEVPTPTLVTESG
                     RALTAHHSVLIVPVLGAHAKDDPSQDLTVPESAAEPLHALAKILAALPKTRKQSELLE
                     AYHDSNERLDEVRQLFTLGYLPLAERALAESLYWRICTGLLKALKRGKPDASRPEIVE
                     LEDKLTELYLCDFSVFQSMLDHWAIGQPFPIVPIDRLDEAPEKRAILVDLTCDSDGKV
                     SHYVSTRDKRFLPVHATVNGTPYYVGFFLMGAYEDIIGDAHNLFGRVTEAHIYADPDE
                     DGDFWIEKIIHGTAVQDMLAQVQYFPNDLHRRMSELVRAKIQQNVVRPTQGMEILDQY
                     MACFPQTTYCDTRDIEREHQR"
     gene            1761..2651
                     /locus_tag="VFL84_14190"
     CDS             1761..2651
                     /locus_tag="VFL84_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HET7609826.1"
                     /translation="MTPRPLKVGLVQMAMGEDPARNLAKAIEGIRDAAKRGAQLVVLP
                     ELFRSRYFCQSEDAEQFKLAEAIPGPSTDTLGELARELQITLVASLFEKRAAGLYHNT
                     AAVIDAERGYLGKYRKMHIPDDPRYYEKYYFTPGDLGFKVFDTKPAQLGVLVCWDQWY
                     PEAARLTAMQGADILIYPTAIGWHPEEKAQYGERQHGAWETVQRGHAVANGCFVIAVN
                     RTGFEPDPRGSGGIEFWGQSFIAGTDGRILVRAPADREVVLVEEIDLAEIDAVRAGWP
                     FFRDRRIDAYTEITRRFGND"
     gene            2849..3700
                     /locus_tag="VFL84_14195"
     CDS             2849..3700
                     /locus_tag="VFL84_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7609827.1"
                     /translation="MTLATVYRLIQQQEAGLLAEWIRSQRAAKRSAGGAYADEQETAA
                     SAEFLKRFMRGLAAGHADTGDASWDATREFLDGLSRSRAKQGFTPKETATFVFSLKEP
                     LFARMRAAIKDADELGTAIWDATLLLDDLGLYTTEVYQKSREELISRQQQELLELSTP
                     VTRLWEGVLAVPLVGTLDSARTQVVMESLLEEIVRSGASIAVMDITGVPTVDTLVAQH
                     LLKTVAAAKLMGAECVISGIRPQIAQTIVHLGVDLGDVVTKSSLADGFRHALGRLGLA
                     VVPAAGR"
     gene            3703..>3848
                     /locus_tag="VFL84_14200"
     CDS             3703..>3848
                     /locus_tag="VFL84_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-anti-sigma factor"
                     /protein_id="HET7609828.1"
                     /translation="MEKIPILRMGRFLLVSIQVDLHDRMAMRLQEDLTEQIVSTGAKG
                     VLID"
BASE COUNT          700 a         1280 c         1264 g          604 t
ORIGIN      
        1 gtgctcctgc ggttccaaga cgtcttgcgc gcccaggttc ggcgcgtcaa cgaggccttc
       61 cgtagcgcga tcgcggaatc gaactacggc aacgtctatc gcggcatcta tccgatcaag
      121 gtcaaccagc tgcacgaggt cgtcgacgag ctgctcgacg ccggccggcc gtacggcatg
      181 ggcctcgagt gcggctcgaa agccgagctg atcgcgtgtc tgccgcacat cgacggcgag
      241 acgctgctcg tctgcaacgg cgtcaaggac cgcacgatgc tgtcgctgat gatcgccggc
      301 cagaagctcg ggcagaacat cgtgcccgtg atggagaagt actcggagtt caccgacgtc
      361 aaggggctcg cgttctcgac gggctacatg tcgcagctcg gcgtgcgcgt gcggctcgcg
      421 acgcgcggcg cgggacggtg ggcggaatcg tcgggcacga actcgaaatt cggcctcaac
      481 gtgtccgagc tcatgcgcat ggtcaacgag ctcgaggcga gcggccagca atcgaagctc
      541 gtgctgctgc actgccacat cggcagccag atcgcggaca tccaggtgct caaggaggcg
      601 acgaaagagg tcgcgcagat ctacgcgcag ctcgtgaagc gcggcatcgc gttgaaatac
      661 ctcgacgtcg gcggcggcct cggcgtcaac tacgacaagg gccatctcga ggaagacgcc
      721 ggcatcaact acgcgctcga ggaatacgcg aacgccgtcg tgttcacggt caaggaagtc
      781 tgcgacgcga acgaggtgcc gaccccgacg ctcgtgacgg aaagcggccg cgcgctgacg
      841 gcgcaccact cggtgctgat cgtgcccgtg ctcggcgcgc acgcgaagga cgatccgagc
      901 caggacctca cggtgccgga gagcgcggcg gaaccgctgc acgcgctcgc gaaaatcctt
      961 gcggcgctgc cgaaaactcg caagcaatcg gagctgctcg aggcttacca cgacagcaac
     1021 gagcggctcg acgaggtgcg gcagctgttc acgctcggct acttgccgct cgccgagcgc
     1081 gcgctcgccg agagcctgta ctggcgcatc tgcacgggac ttctgaaggc gctgaagcgc
     1141 ggcaagcccg acgcatcacg gcccgagatc gtcgagctcg aggacaagct cacggagctc
     1201 tacctctgcg atttctcggt gttccagtcc atgctcgatc actgggccat cggccagccg
     1261 ttcccgatcg tgccgatcga ccggctcgac gaggcgcccg agaagcgcgc gatcctcgtc
     1321 gacctgacct gcgattcgga cggcaaggtc tcgcactacg tgtcgacgcg cgacaagcgc
     1381 ttcctgcccg tgcacgcgac cgtcaacggc acgccgtact acgtcggctt cttcctgatg
     1441 ggcgcgtacg aggacatcat cggcgacgcg cacaatctgt tcggccgcgt gaccgaggcg
     1501 cacatctacg ccgaccccga cgaggacggc gacttctgga tcgagaagat catccacggc
     1561 acggccgtgc aggacatgct cgcgcaggtg cagtacttcc cgaacgacct gcaccggcgc
     1621 atgagcgagc tcgtgcgcgc gaagatccag cagaacgtcg tgcggccgac gcaaggcatg
     1681 gagatcctcg accagtacat ggcgtgcttc ccgcagacga cctattgcga cacgcgcgac
     1741 atcgagcgcg agcaccagcg atgacgccgc gcccgctgaa agtcgggctc gtgcaaatgg
     1801 cgatgggcga ggatccggcg cggaaccttg ccaaggcgat cgaaggcatc cgcgatgccg
     1861 cgaagcgcgg cgcgcagctc gtcgtgctgc ccgagctgtt tcgctcgcgc tacttctgcc
     1921 agagcgagga cgcggagcag ttcaagctcg ccgaggcgat cccggggccg agcacggaca
     1981 cgctcggcga gctcgcacgc gagcttcaga tcacgctcgt cgcgagcctg ttcgagaagc
     2041 gcgcggccgg tctctaccac aacacggcgg ccgtcatcga tgcggaacgc gggtatctcg
     2101 gcaagtaccg caagatgcac atccccgacg acccgcggta ctacgagaag tattacttca
     2161 cgccgggcga cctcgggttc aaagtcttcg atacgaagcc ggctcagctc ggcgtgctcg
     2221 tctgctggga ccagtggtac cccgaggccg cgcgcctgac ggcgatgcag ggcgccgaca
     2281 tcctgatcta tccgacggcg atcggctggc accccgagga gaaggcgcag tacggcgagc
     2341 gccagcacgg cgcctgggag accgtgcagc gcggccatgc cgtcgcaaac ggctgcttcg
     2401 tgatcgccgt gaatcgcacc ggcttcgagc ccgacccgcg gggctcgggc ggcatcgagt
     2461 tctggggcca gagcttcatc gccggcaccg acgggcgcat cctcgtccgc gcgcccgccg
     2521 accgcgaggt cgtgctcgtc gaggagatcg acctcgccga gatcgacgcc gtccgcgccg
     2581 gctggccgtt cttccgcgat cggcgcatcg acgcctacac cgagatcacg cgccgcttcg
     2641 gcaacgactg aacccgttag agccccatcc gccgccgccg tcgcgacgcg cgatcggccg
     2701 cggcgccgtc cggcatggga tttgcttcta ggggtcaaag gaatcgaact tcaataaccc
     2761 agggccaggc aacccgcgtt gccgtgggcc tgcttgcgtt tgctcgatcc caaatgctgc
     2821 acgagctttc caaactttgg aggacagcat gacgcttgcg accgtctatc gcctgataca
     2881 acaacaggaa gcgggcctgc tcgccgagtg gattcggtcg caaagggcgg caaagcgttc
     2941 ggcgggcggg gcgtacgccg acgagcaaga gaccgccgcg tcggccgagt ttctgaagcg
     3001 cttcatgcgc gggctcgcag ccggacatgc cgataccggc gacgcgtcct gggacgcgac
     3061 gcgcgagttc ctcgatggtc tgtcgcggtc gcgggccaag caggggttca cgccgaagga
     3121 gaccgcgacg ttcgtcttct cgctcaagga gccgctgttc gcgcgcatgc gcgccgcaat
     3181 caaggacgcc gacgagctcg gcaccgcgat ctgggacgcg acgctgttgc tcgacgacct
     3241 cggcctctac acgaccgagg tgtaccagaa gagccgcgag gagctgatct cgcgtcagca
     3301 gcaggagctg ctcgagctct cgacgccggt cacgcggctc tgggaaggcg tgctcgccgt
     3361 gccattggtc ggaacgctcg atagcgctcg cactcaggtc gtcatggagt ccttgctcga
     3421 ggagatcgtt cgctccggcg cttcgatcgc cgtcatggac atcacgggcg tgcccaccgt
     3481 cgacacgctc gtcgcacaac atctcctgaa gaccgttgcc gccgcgaagc tcatgggagc
     3541 ggagtgcgtc atcagcggca tccgccccca gatcgcgcag acgatcgtgc atttaggcgt
     3601 cgacctcggc gacgtcgtca cgaagtcgtc gctggccgac gggttccgcc acgcgctcgg
     3661 ccgcctgggc ctagcggtcg ttccggctgc gggccgttga ccatggagaa aatcccgatc
     3721 ctgcgcatgg gcaggttttt gctcgtctcg attcaagtcg atctgcacga ccgcatggcg
     3781 atgaggctcc aggaagacct cacggagcag atcgtcagta ccggagccaa gggagtcctg
     3841 atcgatat
//
LOCUS       DASSNN010000756         4946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_702358, whole genome shotgun sequence.
ACCESSION   DASSNN010000756 DASSNN010000000
VERSION     DASSNN010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702358"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFL84_14205"
     CDS             <1..373
                     /locus_tag="VFL84_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7609829.1"
                     /translation="RRLALMKPTALLINTARGAVVDLDALCDALDSHALAAAALDVLP
                     EEPLPRDARILRFGDRVLLSPHMVAANQGGTLGAAIPWAAEAALAVLRGELPERIYNT
                     EAVVKWQARFGGKSLLGARRG"
     gene            476..1705
                     /locus_tag="VFL84_14210"
     CDS             476..1705
                     /locus_tag="VFL84_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7609830.1"
                     /translation="MYDFANSGYTTVVITAVFNAYFVAVVCEGQSWGTFAWTLALSIS
                     YALVIISAPIIGAYADAHAAKKKLLAITTSGCVLGTALLALVGPGDIALAFALIVLSN
                     VFFGTGENIAAAFLPELATGKAMGKVSAWGWSLGYIGGLVTLGICLAYVFWARSAGQT
                     EAEFVPVTMLITAGVFAVASSFTFLVLKERAVPQPAEKGIAVRALARVGQTLQQARRY
                     KDLLRFLGCIVLYQSGVQTVIALAAIYAQQAMGFSTQDTIVLVLLVNITASLGAYLFG
                     QWQDRLGHRKTLTLTLLGWCSMVVIAYLSTGQMGFWIAANIAGLCLGASQSAGRALVG
                     YLAPADRHAEFFGLWGLAVKLASILGPLSYGAVTWITDNNHRTAMLITGGFFVLGLAV
                     LASVDPERGRRAAIAAA"
     gene            1922..2902
                     /locus_tag="VFL84_14215"
     CDS             1922..2902
                     /locus_tag="VFL84_14215"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET7609831.1"
                     /translation="MADKKILYVFPGQGSQYRGMGSDLVEAFPVARDLYARASEVVGY
                     DMTELSFRDPREQLNQTRFTQPALLTHEVACLESFRSLVGDRAKPALGAGHSLGEYAA
                     LVNAGALTFEAALKLVQRRGELMSEFGRGGMVATTLELPAAQALADKYFCGIGGCNLP
                     DQTVIAGETKDLDALSAEMATLYPNKRGVRLNTEGAFHTYLMVSAAQEFRKVLEQTEF
                     GVLGVDTLSNYTGKLHESASNAIRSRLFFQLFNPVKWVSCINTAVDAGIDTVIELGGG
                     IGKGEGPDGKRPNLESIVKKSLKWREHQAQYLPAISAATIRATAEQLSAG"
     gene            2990..4702
                     /locus_tag="VFL84_14220"
     CDS             2990..4702
                     /locus_tag="VFL84_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HET7609832.1"
                     /translation="MAGLKDPHGGVLKNLYLDEAGVKEEKARARDLPSWDMNMRQLCD
                     IEMLLNGAFSPLEGFLGEADYRRVLSDMRLTSGLLWPMPITLDVTEQFAEKRKVGDTI
                     ALRDSEGVLIATMDIASIYRPNFDEEAKAVFGTTDQKHPAVSYLKTRSGPVYLGGKLR
                     GVEVPGHYDFKHLRDTPRELRDRFQKLGWRRIVAFQTRNPMHRAHQELTFRAAKDVEA
                     NLLIQPVVGMTKPGDVDHFTRVRCSEHLLKAYPEQTTALSLLPLAMRMGGPREAMWHA
                     IIRKNYGCTHFIVGRDHAGPGKDSAGKPFYGPYDAQELLKKHEKELDITVVPFKNMVY
                     VEDKAQYFPEDEVEAGTRTLDISGTELRRRLQEGAEIPDWFSFPDVVAELRRTHPPRH
                     KQGFTVFFTGLSGSGKSTVANALMVKLMEMGGRPVTLLDGDVVRKHLSSELGFSKEHR
                     DLNIQRIGYVASEITKNGGIAICAPIAPYAATRKRVREMIEPLGGFIEIHVATPIEVC
                     EARDRKGLYAKARAGILKGFTGIDDPYEAPENPEMRLDTTESTPDMSAHRIIVKLESL
                     GFIK"
BASE COUNT          899 a         1567 c         1640 g          840 t
ORIGIN      
        1 gcgcaggctc gcgctgatga agccgacggc gctgctgatc aataccgcgc gcggcgccgt
       61 cgtcgatctc gacgcgctct gcgacgcgct ggacagccac gcgctcgccg cggcagcgct
      121 cgacgtgttg cccgaggagc cgctgccccg cgacgcacgg atcctgcggt tcggcgatcg
      181 cgtgctgttg tcgccgcaca tggtggccgc gaaccagggc ggcacgctcg gggccgcgat
      241 tccgtgggcc gccgaagcgg cgctcgcggt gctgcgcggc gagttgccgg agcgcatcta
      301 caacaccgag gccgtcgtga agtggcaagc gcgcttcggc ggcaagtcgt tgctcggcgc
      361 ccgccgcggt tgattcgaag cgtgatcgga aaaattactt gcaggcagga tttgacgagt
      421 gagtagcagc ggcttgcagc cgggggtgcg cccgcgcgag gtttgggcgt gggcgatgta
      481 cgacttcgcc aactcgggct acacgaccgt cgttatcacg gctgtcttca acgcctattt
      541 cgtcgcggtc gtctgcgagg ggcaaagctg gggaacgttc gcgtggacac tcgcgctgtc
      601 gataagctat gcgctcgtca tcatcagcgc gccgatcatc ggcgcctatg ccgacgccca
      661 cgccgcgaaa aagaagctgc tcgcgatcac gacctcgggg tgcgtgctcg gcacagcgct
      721 gctggcgctg gtcggccccg gggacattgc gctcgcgttc gcgctgatcg tgctgtcgaa
      781 cgttttcttc gggaccggcg agaacatcgc cgccgcgttc ttgcccgagc ttgcgacggg
      841 aaaggcgatg ggcaaggttt cggcatgggg ttggtcgctt ggctacatcg gcggcctcgt
      901 gacgctcggc atctgcttgg cctacgtgtt ctgggcgaga agcgcgggac aaacggaagc
      961 ggagttcgtt ccggtcacga tgttgatcac ggccggcgtg ttcgccgtgg cgagctcgtt
     1021 cacgttcttg gtgctcaagg agcgcgcggt tccgcaaccg gcggagaagg gcatcgcggt
     1081 gcgcgcgctg gcgcgcgtag gtcagacgct gcagcaggcg agacgctaca aagatctgct
     1141 gcgctttttg gggtgcatcg tgttgtacca gtccggcgtg caaacggtga tcgcgctcgc
     1201 ggcgatctat gcgcagcagg cgatgggctt ctcgacgcaa gacacgatcg tgctcgttct
     1261 gctcgtcaac atcacggcga gcctcggtgc gtacctgttc gggcaatggc aggaccggtt
     1321 gggccaccgc aagacgctga cgctgacgct gctcggctgg tgctcgatgg tcgtgatcgc
     1381 atacttgagc acgggccaga tgggcttctg gatcgccgcg aacatcgccg gcctctgctt
     1441 gggcgcgagc caatcggccg gccgcgcgtt ggtcgggtat ctcgcacccg ccgaccggca
     1501 cgcggaattc tttgggctct ggggcctcgc cgtgaagctt gcctcgatcc tggggccgtt
     1561 gagctacggc gccgtcacct ggatcaccga caacaaccac cgtaccgcga tgctgatcac
     1621 gggcgggttc ttcgtgctcg ggctggcggt gctcgcgagc gtcgatcccg agcggggccg
     1681 acgcgccgcc atcgcggccg cctgaagccc cccctgcggc tgcatagcct cccccgcagg
     1741 tgccgttccc taggaggtgc cggtgttacg gttcggttca accgtagctc cggcacttcg
     1801 gttgaaccga accgtaacac cggcaccgaa cggcataacg cgattccagc ttagtcttgg
     1861 catagcccgc gggttcgggg catcgtaaca actgcacgag cgtccagggc aagaccgaag
     1921 cgtggctgac aagaagattc tctacgtttt tcccggccaa ggttctcagt atcggggcat
     1981 gggctccgac ctcgtggaag cgtttccggt cgctcgcgat ctctatgcga gggcgagcga
     2041 ggtcgtcggt tacgacatga ccgagctcag cttccgcgac ccgcgcgagc agctgaatca
     2101 aacccgtttc actcaacccg cgctgctcac gcacgaagtc gcgtgcctag agtcgttccg
     2161 cagcttggtc ggcgatcgcg ccaaacccgc gctcggcgcc ggccacagcc tcggcgagta
     2221 cgcggcgctc gtgaatgcag gagcgctgac gttcgaggct gcgctgaagc tcgtgcagcg
     2281 gcgcggcgag ctcatgagcg agttcggccg cggcgggatg gtagcgacga ctctcgagct
     2341 gcccgccgcg caagcgctcg ccgacaaata cttctgcggt atcggcggct gcaacttgcc
     2401 ggaccagacg gtgattgccg gcgaaaccaa ggatctcgac gcgttgagcg ccgagatggc
     2461 gactctttac ccgaacaagc gcggcgtgcg gttgaacacc gaaggcgcgt tccacacgta
     2521 tctcatggtc agcgcggcgc aggagtttcg caaagtgctc gagcagacgg agttcggcgt
     2581 gctcggcgtc gatacgctct cgaactacac gggcaagctg cacgaatccg cgagcaacgc
     2641 gatccgctcg cggttgttct tccagctctt caacccggtc aagtgggtct cgtgcatcaa
     2701 cacggccgta gacgccggca tcgataccgt catcgagctc ggcggcggca tcggcaaggg
     2761 cgagggtccc gacggcaagc ggccgaacct cgagtcgatc gtcaagaaat ccttgaaatg
     2821 gcgcgagcac caagcacaat acctgcccgc gatcagcgcg gcgacgatcc gcgcgacggc
     2881 cgaacaactg tcggccggtt aaatcgatga atcgaaccgc tgcaccggcc cgaggccgaa
     2941 cggcccgagc gtgccggagc gcaccgcaat gacgatggag taaacgcaaa tggctggctt
     3001 gaaagatccc cacggtgggg tgctgaagaa cctgtacctc gacgaagcgg gcgtgaagga
     3061 agagaaggcc cgcgcgcgag acctgccgtc gtgggacatg aacatgcggc aactctgcga
     3121 catcgagatg ctgctgaacg gcgcgttctc gccgctcgag gggttcttgg gcgaggccga
     3181 ttatcgacgc gtgctatccg acatgcgtct cacgagcggc ctgctgtggc cgatgccgat
     3241 cacgctcgac gtcaccgagc agttcgcaga gaagcgcaag gtcggcgaca cgattgcgct
     3301 gcgcgatagc gaaggcgttc tgatcgcgac gatggacatc gcgagcatct accgcccgaa
     3361 tttcgacgag gaagcgaagg ccgtgttcgg cacgacggac cagaagcacc cggccgtcag
     3421 ctatctgaag acgcggagtg gtcccgtcta tctcggcggc aagctgcgcg gcgtcgaggt
     3481 tcccggtcac tacgacttca agcatttgcg cgacacgccg agagagcttc gcgaccgatt
     3541 ccagaagctc ggctggcgcc gcatcgtcgc gttccagacg cgcaatccaa tgcaccgcgc
     3601 gcaccaagag ctcacgttcc gcgcggccaa ggacgtcgaa gcgaacctgc tgatccagcc
     3661 cgtcgtcggc atgacgaagc ccggcgacgt cgatcacttc acgcgcgtgc gctgttccga
     3721 gcatctgctg aaggcctatc ccgagcagac gaccgcgctc tcgttgctgc cgctcgcgat
     3781 gcgcatgggc ggcccgcgcg aggcgatgtg gcacgccatc atccgcaaga actacggctg
     3841 cacgcacttc atcgtcggac gcgatcacgc cggtccggga aaggacagcg ccggtaagcc
     3901 gttctacggt ccgtatgacg cgcaggagct gctgaagaag cacgagaaag agctcgacat
     3961 caccgtggtc ccgttcaaga acatggtcta cgtcgaggac aaggctcagt acttccccga
     4021 ggacgaagtc gaggcgggca cgcgcacgct cgacatctcc ggcaccgagc tgcgccggcg
     4081 gctccaggaa ggcgccgaga tcccggactg gttctcgttt cccgacgtcg tcgccgagct
     4141 gcgccgcacg catccgccgc ggcacaagca gggcttcacg gtgttcttca cggggctctc
     4201 gggctcgggt aagtcgaccg tcgccaatgc gttgatggtc aagctcatgg agatgggcgg
     4261 ccggcccgtg acgctgctcg acggcgacgt cgttcgcaag catctatcga gcgagctcgg
     4321 cttctcgaaa gagcaccgcg acctcaacat ccaacgcatc ggctacgtcg caagcgagat
     4381 cacgaagaac ggcggcatcg cgatttgcgc gccgattgcg ccgtatgcgg cgacgcgcaa
     4441 gcgggtccgc gagatgatcg agccgctcgg cggcttcatc gagattcatg tcgcgacgcc
     4501 gatcgaagtc tgcgaggcgc gcgaccgaaa aggtctctac gcgaaagcgc gcgccggcat
     4561 cctcaagggc ttcacgggca tcgacgatcc gtacgaggcg ccggagaatc ccgagatgcg
     4621 gctcgatacg accgagtcga cgcccgacat gtcggcgcat cgcatcatcg tcaagctcga
     4681 gtcgctcggc ttcatcaagt aagccccgtg acgcccgaag ggcggaaggc gcgccgaacg
     4741 gcgcgagacg ccactgtgcc cgcggaaagc catcgagagc gagcccgaga gtgcagtcgc
     4801 gagagccgat ttcttcgacg aatcaatggc atgcgtggac ataatgcgac cgcactggaa
     4861 gtcagtgctc cgtcgcggca gaatcgactt gcagaacgct aagaagaaca acaactttgc
     4921 gcgcgtttgg ctgtatcttg ctggcg
//
LOCUS       DASSNN010000757         4849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_871255, whole genome shotgun sequence.
ACCESSION   DASSNN010000757 DASSNN010000000
VERSION     DASSNN010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871255"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFL84_14225"
     CDS             <1..708
                     /locus_tag="VFL84_14225"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7609833.1"
                     /translation="INEWLFHQCGAFWSEPMSVHFMLSQPEVVDELATSARRAFTAHL
                     DKYVGPTRWHQQGRAKPLPAFGWKDPRNTFTLPVWKQVFPNLRVIHIVRHGIDVAASL
                     SQRHLAALLAATGGSAPNALTVIRDDAFGILSSRRGWTLTEALTMWEQYLDKARLECA
                     ALGERAMEIRYEDLLSEPQRVIPIVAEFCGVPAPADAASVTALERSRAFAFRRDPDLV
                     DFARSARPALARYGYAA"
     gene            795..1397
                     /locus_tag="VFL84_14230"
     CDS             795..1397
                     /locus_tag="VFL84_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609834.1"
                     /translation="MSRIYASTILALAALQLPAAPAIAQVIFAPGRGGGIDTAIQGRA
                     TTRDDIYTCQAAPGGSRIRENCEVVTEIEQLEQQQKMAKLLKPPRLLQCGASTTTEFR
                     QLDTIARVNGTLEIRDCGAASGTVTIAVVVKDESGAQEPREFTETWQRDDERDVSFSA
                     DYPIGEKAELVDVRLSGLTCTCTDPFAQPGPPAAGEGTPD"
     gene            1577..2545
                     /locus_tag="VFL84_14235"
     CDS             1577..2545
                     /locus_tag="VFL84_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609835.1"
                     /translation="MLDSRTFTTKLALAAIAALLSGAAAAQEAPAGSAEADGGTSQAI
                     EEVVVRGRRLEDIKSDLRIHIRDFIGQVVAKPPGRGFARWYRKVCVGVYNLENNAAQY
                     VVDRVSKLALEVGLTPGEPGCAPQINIVFATNAKETAAAMVESEPRMFMPVAGHAGMD
                     LGRVALEEFAQSDKAVRWWHVSLPVDARTGGAAIELDKNCGQAFCPPTVNVAGPSRIH
                     NGTRDDMIYVIVIVDATQLNGTTWQQLGDYLAVVSLCQIDPHANPASFDSILNLFTNP
                     KAYSGLTDWDRSYVRALYEFDQERIESMQTNEIVGRIAARELESAQ"
     gene            complement(2555..4309)
                     /locus_tag="VFL84_14240"
     CDS             complement(2555..4309)
                     /locus_tag="VFL84_14240"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7609836.1"
                     /translation="MPQQSIDEAFAAALEHHRAGRLGNAEQGYRAILARAPEHADSLN
                     LLGVIALQVGDLPSALALVRRAVELRPDAAVCRNNLGQVLERHGRDDEAAECYEAAIR
                     LDPTYAEAHNNLGVLRARRDRLAEAEALYRKAVELDPNYAEPHTNLGNLLKDRGELDA
                     AIASYRRAVEIRPDLSQLHSNLLLTLHYHPGCSPAELLREHLVWAERHVAPLKPARRP
                     HANVADPERRLRVGYVSPDFREHPVARFMLPLLQEHDRRQVEVFAYSDVTAPDPVTGI
                     VRRHADQWRDVAALGDDALAEAVRADGIDILVDLAAHSGHNRMLTFARRPAPVQLTYL
                     AYCSTTGNDAIDHRITDRFLDPPGEEGSYTEASIRLPYCYWCYSAPPLSLPPATRRPG
                     PPTFGCLNNFAKVSDVTLALWVRLLRRVPEAELLLYARTEAHRERVHRALRAGGIDEA
                     RVTFVGRQSLALYLETYRDVDVALDPYPYGGGTTSCDALWMGVPVVSLAGSTAVSRAG
                     STLLSNVGLERLVATTGEQYVERAAELIRDQARLAELRRELRRSLESSPVMNAPQFAR
                     DFEAALRAAWRAWCAGRS"
     gene            complement(4379..>4849)
                     /locus_tag="VFL84_14245"
     CDS             complement(4379..>4849)
                     /locus_tag="VFL84_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609837.1"
                     /translation="AAAGAVFSLAAGQDAFAQAPAEPVSVDVSECVKLTTPEERLACF
                     EKQVEGSRTSPAAQGAASASPPPPASSASSDARGPDRKAEPADVEARIAELRETVPNA
                     YLITLDNGQVWRQTVPKAYPLQAGHPVRIYYSRWRSYRLTNETLKSFIQVERVR"
BASE COUNT          796 a         1627 c         1665 g          761 t
ORIGIN      
        1 atcaacgagt ggctcttcca tcagtgcgga gcgttctggt ccgagccgat gtcggtccat
       61 ttcatgctgt cgcagcccga ggtcgtcgac gagctcgcga cgagcgcacg tcgagcattc
      121 accgcgcacc tcgacaagta cgtgggcccc acgcgttggc accagcaagg gcgcgcgaag
      181 ccattgccgg ccttcggttg gaaggacccg cggaacacgt tcacgctgcc ggtgtggaag
      241 caggtattcc cgaatctgcg ggtcatccac atcgtgcgtc acggcatcga cgtagccgcg
      301 agtctctcgc aacggcactt ggcggcgctg ctcgcggcga ccggcggatc tgctcctaac
      361 gcactgaccg tgatccgcga cgacgcattc ggcatcctat cgtcgcggcg cggttggacg
      421 ctcaccgagg cgctgacgat gtgggagcag tacctcgaca aggcgcgtct cgaatgcgcg
      481 gcgctaggcg agcgcgcaat ggagatccgg tacgaggatc tgttgagcga gccgcagcgc
      541 gtcattccga tcgtcgcgga gttctgcggt gttcccgcgc ccgcggacgc cgcatcggtc
      601 accgccctcg agaggagccg ggccttcgcg ttccgccgcg atcccgatct cgtcgatttc
      661 gcgcgttcgg cgcgccctgc gctcgcgcgt tatggttatg cggcttgaac ccgccgaccc
      721 ccggcgcgga cgcatgctag tcttggggtt gcgttagctc gattacgaca gcccagtcgt
      781 tcggaggctg aaaaatgagt cgaatctacg ccagtacgat ccttgcactg gccgcgctgc
      841 aactgcccgc cgcgccggcc atcgctcagg tcatcttcgc gcccggccgc ggcggcggca
      901 tcgacaccgc cattcagggg cgcgccacca ccagggacga catctacacc tgccaggcgg
      961 cgccgggcgg atcgcgcatc agggaaaact gcgaggtcgt cacggagatc gagcagctcg
     1021 agcagcaaca gaagatggcc aagctgctca agccgccgcg gctcttgcaa tgcggtgcca
     1081 gcacgacgac cgaattccgg cagctcgaca cgatcgcgcg cgtcaacggc acgctcgaga
     1141 tccgcgactg cggggccgcg tcgggcaccg tcacgatcgc cgtcgtcgtc aaggacgaga
     1201 gcggcgcaca agagccgcgc gagttcaccg aaacttggca acgcgacgac gaacgtgacg
     1261 tcagcttctc ggccgactac ccgatcggcg agaaggccga gctcgtcgac gttcgcctga
     1321 gcggcctgac gtgcacgtgc acggacccct tcgcgcaacc cgggccgccc gcggccggag
     1381 aaggtacgcc ggattagcgc aagatcagtg aacgtgcgcg tgcgcggcct gacgtgcacg
     1441 tgcgccgata ccgcgcaatg agcagtgcgc tgaccgatcg gccgttcaag cgagtgtgaa
     1501 gtttcctagc ctgctcccgg cgccatggtt ttcctacggt acactccgcc gacctcgccc
     1561 gctctggact cgtcggatgc tcgatagcag gacctttacc accaagctcg ctctggcggc
     1621 gatcgcggcg ctgctgagcg gggccgcggc ggcacaggaa gcgccggccg gctcggctga
     1681 ggccgacggc ggcacgtcgc aggccatcga ggaggtcgtc gtgcgcggtc gtcggctcga
     1741 ggacatcaag tcggaccttc gcatccacat cagagacttc atcggccagg tcgtcgcgaa
     1801 gccgccgggg cgcgggttcg cacgctggta ccgcaaggtc tgcgtcggcg tctacaacct
     1861 cgagaacaat gccgcgcagt atgtcgtgga ccgcgtctcg aagctcgcgc tcgaagttgg
     1921 gctcacgccc ggcgagcccg gttgcgcgcc gcaaatcaac atcgtgttcg cgaccaatgc
     1981 gaaggaaacg gccgccgcga tggtcgaaag cgagccgcga atgttcatgc ccgtggcagg
     2041 acacgccggc atggacctgg gccgcgtcgc gctcgaggag ttcgcgcagt ccgacaaggc
     2101 cgtgcgctgg tggcacgtga gcttgccggt cgacgcgcgc accggcggcg cggccatcga
     2161 gctcgacaag aactgcgggc aggcattctg cccgccgacc gtcaacgttg cgggcccgtc
     2221 gcgcattcat aacggcacgc gcgacgacat gatctacgtc atcgtcatcg tggacgcgac
     2281 gcagctcaac ggcacgacgt ggcagcagct cggcgactat ctcgccgtcg tatcgctgtg
     2341 ccagatcgac ccgcacgcga acccggcatc gttcgacagc atcctgaacc tgttcacgaa
     2401 tccgaaggct tattccggcc tcaccgattg ggaccggtcc tacgttcgcg cgctctacga
     2461 gttcgatcag gaacggatcg aatcgatgca gacgaacgag atcgtcgggc gcatcgcggc
     2521 gagggagctc gaatccgctc agtgagcggc agccttacga tcggcccgcg caccaggcgc
     2581 gccatgccgc tcgcagcgcc gcctcgaaat cgcgcgcgaa ctgcggcgcg ttcatgacgg
     2641 gcgacgattc gaggctgcgc ctgagctcgc gccgcagctc cgcgagccgt gcctgatcgc
     2701 ggatcagctc ggccgcgcgc tcgacgtact gctctccggt tgtcgcgacg agccgttcga
     2761 ggccgacgtt cgacagcaac gtcgagcccg cgcgcgagac ggccgtgctg ccagcgagac
     2821 tgacgacggg cacgcccatc cacagcgcat cgcacgacgt cgttccgccg ccgtacgggt
     2881 acgggtcgag cgcgacgtcg acgtcgcgat aagtctcgag ataaagcgcc agcgactggc
     2941 ggccgacgaa ggtcacgcgt gcctcgtcga tgccgccggc gcgcaaggcg cggtgcacgc
     3001 gctcgcgatg ggcctcggtg cgcgcataga gcaggagctc tgcctcgggc acacggcgaa
     3061 gcaggcggac ccatagcgcg agagtgacgt cgctgacctt ggcgaaattg ttgaggcagc
     3121 cgaacgtggg agggcccggg cggcgagtgg ccggcggcag cgaaagcggc ggcgccgagt
     3181 agcaccaata gcagtacgga agccggatcg aggcctcggt gtagctgcct tcctcgccgg
     3241 gcgggtcgag gaagcggtcg gtgatccgat gatcgatcgc gtcgttgccc gtcgtgctgc
     3301 agtacgcgag atacgtgagc tggaccggcg cgggcctgcg cgcaaacgtc agcattcggt
     3361 tgtggcccga atgcgcggcg aggtcgacga ggatgtcgat gccgtcggcg cgcacggctt
     3421 cggcaagcgc gtcgtctccg agcgccgcga cgtctcgcca ctgatccgcg tgccggcgca
     3481 cgatcccggt cacgggatcc ggcgccgtga cgtcggagta cgcgaagacc tcgacctgcc
     3541 ggcggtcgtg ctcttgcagc agcggcagca tgaagcgcgc gacgggatgc tcgcgaaaat
     3601 cgggtgagac gtaccccact cgcaagcggc gctcggggtc ggcgacgttc gcatgcgggc
     3661 ggcgtgccgg cttcaacggc gcgacgtgcc gctcggccca tacgagatgc tcgcgtaaga
     3721 gctcggccgg cgagcagccc ggatggtagt gcagcgtcag cagcaggttg ctgtgcagct
     3781 gcgacaagtc gggacggatc tcgacggcgc gccgatacga ggcgatcgcc gcgtcgagct
     3841 cgccccgatc cttgagcagg ttgcctaagt tcgtgtgggg ctctgcgtaa ttcgggtcga
     3901 gctcgacggc tttgcggtag agagcctcgg cctccgcaag ccgatcgcgc cgcgcgcgca
     3961 gcacacccaa gttgttgtgc gcttcggcgt aagtaggatc cagccggatt gccgcttcgt
     4021 agcactcggc cgcttcgtcg tcgcgtccgt gacgctcgag gacttggccc agattgtttc
     4081 ggcacaccgc cgcatcgggc cgcagctcga cggcccgtcg aacgagcgcg agcgcggacg
     4141 ggagatcgcc gacctgcaac gcaatgactc cgagaaggtt cagcgaatct gcatgctccg
     4201 gcgcgcgcgc gagtatggcg cgatagccct gctccgcgtt gcccaaccgg cccgctcgat
     4261 ggtgctcgag cgcggccgcg aacgcttcat cgatgctctg ctgcggcatc cgcctcccgc
     4321 ggcgatggac gtaggccgcg tagcgtaccg tatgccgttg cgggttggag gccgcgcgtc
     4381 agcgcactcg ctcgacctgg atgaagctct tcagcgtctc gttcgtgaga cggtacgagc
     4441 gccaccgcga gtagtagatc cgcacggggt gcccggcctg taacgggtaa gccttcggca
     4501 cggtttggcg ccacacctgg ccgttgtcga gcgtgatcag gtacgcgttc ggcaccgttt
     4561 cgcggagctc ggcgatccgc gcctcgacgt cggccggctc cgctttgcgg tcagggccgc
     4621 gcgcgtcgct cgaggcactg ctcgcgggcg gcggcgggct cgccgaagcg gcgccctgcg
     4681 cggccgggct ggtgcgcgag ccctcgacct gcttctcgaa gcaggcaagg cgctcctccg
     4741 gcgtggtgag cttgacgcac tcgctgacgt ccacgctgac cggctccgcc ggcgcctgcg
     4801 cgaaagcgtc ctggccggcc gcgagcgaaa aaacagcgcc ggcggcggc
//
LOCUS       DASSNN010000758         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1879444, whole genome shotgun sequence.
ACCESSION   DASSNN010000758 DASSNN010000000
VERSION     DASSNN010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879444"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VFL84_14250"
     CDS             complement(<1..1126)
                     /locus_tag="VFL84_14250"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7609838.1"
                     /translation="MAERIGAWLAAWASGVVARPRLTVAATLVLVALAAWYAAGNLGV
                     NTDTANMISPTIPWRQHYNDYRDAFPLRDRNLLIVIDAPTPARADDLAAGLLAELRKR
                     PDEYRAPLLAGEGEFFERNGLLYLPTANLAELTDRLAAAQPLLGLLQKKLDGAAVIDV
                     ARQTLAADGAGETAELASFYEELARTIDAAAEGQASALAWDRLISTTPARAPGTRRII
                     TLQPAVDFRRMQPAAEAIADVRAIAARLDPAGEARVRLTGTVAMEHEELLSVSRGAGL
                     GALATFLMVAVVLYAALRSWRLLLASIVTLIAGLSLTAAFAAVAVGQLNLLSIAFVVL
                     NVGLGSDYVIHVLLRYRELVAEGHATNAALVEAVRDVGSSL"
     gene            complement(1130..1876)
                     /locus_tag="VFL84_14255"
     CDS             complement(1130..1876)
                     /locus_tag="VFL84_14255"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine phosphorylase"
                     /protein_id="HET7609839.1"
                     /translation="MSFDEPVHRDGAFTIGFIAALALECTSLRRHSPRTERWLVVQSG
                     PGAARAAAAASRAIEAGAQLLVSWGLAGGLGVGVAPGTVIAPRRVLAQGAEPLSVDPA
                     WHSRLALLAADLGVHRGDLLTVAEPLESPAAKRAAAAATNAVAVDMESAGIAAAAAHA
                     RLPFVALRVVVDAVDDALPRGAADWVDEQGHRRKSAVLRAVVDARGWGALLTLARRYR
                     VASGVLNRLAGALAAQPMLGAGGAALQAGS"
     gene            complement(1866..>2358)
                     /locus_tag="VFL84_14260"
     CDS             complement(1866..>2358)
                     /locus_tag="VFL84_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HET7609840.1"
                     /translation="NILKDIWDDRQAGACWLPRSVFARGTMDLERLEAWHTQPVFHDG
                     LRELIGVGHAHLRNALEYTCLIPKRETGIRRFCLWAIGLAVLTLRKIHRNPAFRSGSE
                     VKVSRRTVKATVLTTNLTLMSNRALRLMFARAADGLPLATDALAAAASGESLRRPGRV
                     DVV"
BASE COUNT          367 a          859 c          840 g          292 t
ORIGIN      
        1 cgagcgacga gccgacgtcg cgcaccgctt cgacgagcgc cgcgttcgtc gcatgccctt
       61 cggctacgag ctcgcgataa cgcaacagca cgtgaatcac gtaatcgctg cccaagccga
      121 cgttgaggac gacgaacgcg atcgacagca ggttcagctg gccgaccgcg acggccgcga
      181 acgcggcggt cagcgacagg cccgcgatca gcgtcacgat cgacgccaac agcaggcgcc
      241 acgagcgcag cgcggcatag aggactaccg cgaccatgag gaacgtcgcg agcgcgccca
      301 gacctgcgcc gcggctcacg ctcaagagct cctcgtgctc catcgcgacg gtgccggtca
      361 gccgcacgcg agcctcgccc gcgggatcga gccgcgcggc gattgcgcgc acgtccgcga
      421 ttgcctccgc ggccggctgc atgcgcctga agtcgacggc cggctgcagc gtgatgatgc
      481 ggcgcgtgcc cggcgcgcgc gccggcgtag tcgagatcag ccggtcccag gcgagcgcgg
      541 acgcctggcc ctcggccgcg gcgtcgatcg ttcgcgcgag ctcctcataa aaggaggcga
      601 gctcggccgt ctctccggcg ccgtcggcgg ccagcgtctg gcgcgcgacg tcgatgacgg
      661 ccgcgccgtc gagcttcttc tgtagcagcc cgagcagcgg ctgcgccgcg gccaagcggt
      721 ccgtcagctc ggcgaggttg gcagtcggca gatacaagag gccgttgcgc tcgaagaact
      781 cgccttcgcc ggcgagcagc ggggcgcgat actcgtccgg ccgcttacgc agctcggcga
      841 gcagcccggc cgcgagatcg tcggcgcgcg ccggcgtcgg cgcatcgatg acgatcagaa
      901 gattgcggtc gcgcagcgga aacgcgtcgc ggtagtcgtt gtaatgctgc cgccacggga
      961 tcgtcggcga gatcatgttc gccgtgtccg tgttgacgcc gagattgccg gccgcgtacc
     1021 aggccgcgag cgcgacgagc acgagcgtcg cggctaccgt gagccgcggc cgcgcgacga
     1081 cgccactcgc ccaagcggcg agccaagcac cgatccgctc cgccatgcgc tagctccccg
     1141 cctgcaacgc ggcgccgccg gcgccgagca tcggctgcgc ggccaacgcg cccgcgaggc
     1201 ggttcagcac gccgctcgcg acgcggtagc gccgcgccag cgtcagcaac gcgccccagc
     1261 cgcgcgcatc gacgaccgca cgcagcacgg cggacttgcg ccggtgcccc tgctcgtcga
     1321 cccaatctgc agcgccgcgc ggcagagcat cgtcgacggc gtcgacgacg acacgcagcg
     1381 cgacgaacgg cagacgcgcg tgcgcggccg ccgcggcgat gcccgccgat tccatgtcga
     1441 ccgcgactgc gttcgtggcc gccgcggccg cgcgcttcgc ggccggcgac tcgagcggct
     1501 cggcgacggt cagcaggtcg ccacgatgaa cgccaaggtc ggccgccagc aacgcgagcc
     1561 gcgaatgcca tgcgggatcg accgaaagcg gctccgcgcc ttgcgcgagg actcggcgcg
     1621 gcgcgatgac ggtgccgggc gcgacgccga cacccaagcc tccggcgagg ccccacgaca
     1681 cgagcagctg cgcgcccgcc tcgatcgcgc ggctcgcggc ggctgccgcg cgcgcggcac
     1741 cgggaccgct ctgcacgacg agccagcgct cggtgcgcgg cgagtgcctg cgcaagctcg
     1801 tgcactcgag cgcgagcgcg gcgatgaatc cgatcgtgaa ggcaccgtcc cggtggacgg
     1861 gctcgtcaaa cgacatcgac tcgtccgggc ctgcgcagcg actcgccgct cgcggcggcc
     1921 gcgagggcgt cggttgcgag cggcagcccg tcggctgcgc gcgcgaacat caggcgcagc
     1981 gcgcgattgc tcatcagcgt caggttcgtc gtcaacacgg tggccttgac ggtgcggcgc
     2041 gacaccttga cctcgctgcc ggagcggaac gcggggttgc gatgaatctt gcgcagcgtg
     2101 agcacggcga ggccgatggc ccagaggcaa aagcgtcgga tgccggtctc gcgcttcgga
     2161 atcaggcacg tgtactcgag cgcgttgcgc agatgcgcat gacccacgcc gatcagctcg
     2221 cgcaggccgt cgtggaacac gggttgcgtg tgccaggcct cgagccgttc gagatccatc
     2281 gtgccgcgcg cgaacacgga gcgcggcagc cagcacgcac cggcctgccg gtcgtcccag
     2341 atgtccttca agatgttc
//
LOCUS       DASSNN010000759         4428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_365713, whole genome shotgun sequence.
ACCESSION   DASSNN010000759 DASSNN010000000
VERSION     DASSNN010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365713"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFL84_14265"
     CDS             complement(<1..438)
                     /locus_tag="VFL84_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HET7609841.1"
                     /translation="MSAADYRPADQLYLWLLTVPADPVLIGELNLVRANRGVSLRYAE
                     AWLERGFALSEDLPLVDEEFLPQEKETAVGAVDDARPDRWGERLIRFLDKPARLSLLE
                     YLYFAGDDRFGALGVSTSATEYLPRRFGPLPALGDAARIQELIR"
     gene            complement(435..815)
                     /locus_tag="VFL84_14270"
     CDS             complement(435..815)
                     /locus_tag="VFL84_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7609842.1"
                     /translation="MPKSPRALESLPPAVTQALARLGENLAVARLRRRESQRAWAKRL
                     GISVPTLIRLERGDPGVGIGIYATALWLIGRVGALPDLAAPSEDRGALESDVRKALRR
                     RAIRSPHSGEARLGRKGSDRSGSA"
     gene            941..1174
                     /locus_tag="VFL84_14275"
     CDS             941..1174
                     /locus_tag="VFL84_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HET7609843.1"
                     /translation="MTKRKQPVTLTLDADVVARARMFTDNLSTFVESLLADYIAREQR
                     CRITEANVMRETVATWNRFAEKHGSFADEHSAL"
     gene            complement(2142..2708)
                     /locus_tag="VFL84_14280"
     CDS             complement(2142..2708)
                     /locus_tag="VFL84_14280"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HET7609844.1"
                     /translation="MNGRMGLFGSLMLLASSASAQGVFSFEGIPLRGEPSVEVNLDES
                     MIKLLGGAAELTGFEGVTNVRVLVYENIAEDMQGVLKFVESTGTKLEGDGWRAVVRVR
                     EEGEQVRIYMKPGTDGTLSGVTVMITESGSNDGDDDGGGSGEAVFVNVSGNIRPEQLG
                     QLASGIGVNGVLNGLPGLQPAAPARAQD"
     gene            complement(2713..3231)
                     /locus_tag="VFL84_14285"
     CDS             complement(2713..3231)
                     /locus_tag="VFL84_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609845.1"
                     /translation="MTQKDLNEEWAWTQLEAWADGSLTGESRARMDAAIAADARLRAA
                     AERAVGVHRALRAAPAVSMPRGLRSRLLAIPSQAPRARRSYFVPALASAAVASAVIAA
                     VLWLNLQPTQPPPDPRVVAAAQDLETAMRYLQKSAVITQGHVESAVGTGLRDAFAASR
                     EALAKDTHETGG"
     gene            complement(3228..3716)
                     /locus_tag="VFL84_14290"
     CDS             complement(3228..3716)
                     /locus_tag="VFL84_14290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7609846.1"
                     /translation="MSTWRFRLAVLMHDRAVFRLASALLRDSREAEDVTQEVFLSYWQ
                     HGENVERPREWLLRVARNACLSRFRRAGRVITQDPAELPEISDGRDPAWHYEHSELAS
                     ELRKLVDVLPEPQRSLLVMFDVHGMDGAACARVLGLNANQVKVYLHRARRKLREQLER
                     AS"
     gene            3859..>4428
                     /locus_tag="VFL84_14295"
     CDS             3859..>4428
                     /locus_tag="VFL84_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609847.1"
                     /translation="MNGSRALVLALLAVAGSAAANHDESVGARFVQPGGIDAGTCLDH
                     HAPCVSIQYALAQAEPGNTVKVGAGIFDLSGVDPETFVHGPVHATGGYGDADHYFESR
                     PLQVQSIIVGVDARYRNALAARGFYWAESVAAAKRNEISFAAGTALQQTQAAAAQCVQ
                     GMAGQFPCRNLDFQSQVALAQFSSRPVSAA"
BASE COUNT          749 a         1516 c         1387 g          776 t
ORIGIN      
        1 gcgaatgagc tcctggatgc gagccgcgtc accgagcgcg ggcaacggcc caaaacggcg
       61 cggcaagtac tcggtcgccg acgtcgagac gccgagcgcg ccgaagcggt cgtcgccggc
      121 gaagtagagg tactcgagca acgacaaccg cgccggcttg tcgagaaagc gaatcagccg
      181 ctcgccccag cggtcgggcc tcgcgtcgtc gactgcgccg accgccgttt ccttttcctg
      241 cggcagaaac tcctcgtcga cgagcggcag gtcttcgctg agcgcgaagc cgcgctcgag
      301 ccaggcctcc gcgtaccgca gcgaaactcc gcggttggcg cgaacgaggt tgagctcgcc
      361 aatgagcacc gggtcggccg gcacggtcaa caaccagaga tagagctggt cggcggggcg
      421 atagtcggcc gcgctcacgc gcttccgctc cgatcggagc ccttgcggcc gaggcgcgcc
      481 tcgcccgaat gcggcgagcg aatcgcgcgc cgccgcaacg ccttgcggac atcgctctcg
      541 agtgcgccgc ggtcctcgct cggcgccgcg aggtcgggca gcgcgccgac cctgccgatg
      601 agccacagcg ccgtcgcgta gatgccgatg ccgacgccgg ggtcgccgcg ctccaagcgg
      661 atcagcgtcg gcaccgagat gccgagccgc ttcgcccaag cgcgttgcga ttcgcggcga
      721 cgcaggcgcg cgaccgcgag gttttcgccg aggcgggcca gcgcctgggt tacggccggc
      781 ggcagggatt ccagcgctct agggctcttt ggcatgacat agagtttatc taaacgtcaa
      841 atttggtcaa ctctatttta ttgataaatt ggggtagatc gggcctggct gaatggtctc
      901 actcgcatac gatgcgcatg cgcacatgga gaaaatctcg atgaccaagc gaaagcagcc
      961 tgtcaccttg acgctggacg cggatgtcgt agcgcgggcg cgtatgttta cggacaacct
     1021 ctccaccttc gtcgagtcac tgctggccga ttacatcgct cgggaacaac ggtgccgcat
     1081 caccgaagcc aacgtcatgc gcgagaccgt ggccacctgg aatcgcttcg ccgagaagca
     1141 cggttccttc gccgacgaac attcggctct ctagcattag cgccgttcgg gcgccggctt
     1201 cactgcatgg cgcaggtcgt cgtggcgagg cgctcgcacg agatcgccgt gcgcaaggtt
     1261 ctcggagctg agacgccact ggtgatgatc agggaatgat gtggaccggt taaccagccc
     1321 catcgagctc gacttcacgc tattcggccc attacttcgc gacgaggccg cgcgcgtaaa
     1381 gctactaggc gacgtgctgc aacttgttgg ctagatcgcg gccggaggtt cgaggaggca
     1441 gcggacggcc gtccgatttg tatagcgcga tggcgtcctc tacgatttca caaagctccg
     1501 cgaacacggc ccgctcatcg agcccgtgac aacctccata gaccaggcct ggagcgctgc
     1561 cgacgtagca ttggtcttcg tcggaccact cgacgatctt cacgtatctc gcgctgtccc
     1621 tcatcgctgt gactcccgta tcgccaaccg cacggctctc acttgatacg gcttggcgtc
     1681 gtcacccgga tcgccggaga ttgtacccgg cttgcgtagg cgcggatgca cgtgcggtgg
     1741 cttccgcgtc cgcctctgtc cacgaatccg gcgctctgaa gatcggcgat cagttccctg
     1801 atctttggcg gcacgagctc tctgttcgga cgctgtgccg cctgccgtcg cgcgtcgcgg
     1861 tggacgacga ctcacgattc ttgcgagaag cgcttgttta gctaacgaga aagacgacgg
     1921 ctgcgccccc acagtaccga ccttggaggg aggtggccgg tgtgttctac cactgggcgc
     1981 agccgtccag cctaatagag caggttccgt gccattcggt tacaacgaaa cggaacgcat
     2041 tgatgcggaa ccgaaaacgc tgcgcgccga ccgccgcgtg cccgtagtcg tttgcgccac
     2101 tagttcgtcg caaacgctac aagtgtcccg attccgcaaa ctcagtcctg cgctctggcc
     2161 ggggcggccg gctgcaagcc cggcagacca ttcaatacac cgttcacgcc gatgcccgag
     2221 gccagctgtc cgagctgctc gggccggatg tttcccgaaa cgttgacgaa tacagcctcg
     2281 ccgctgccgc cgccgtcatc gtcgccgtcg ttgcttccgc tttccgtgat catgaccgtg
     2341 acgcccgaca gcgtgccgtc ggtaccgggc ttcatgtaga tgcgaacctg ctcgccttcc
     2401 tcgcgcacgc gtacgacggc acgccagccg tcgccctcga gcttcgtgcc cgtcgattcg
     2461 acgaacttga gcacgccttg catgtcctcg gcgatgttct cgtagacgag gacgcggacg
     2521 ttagtgacgc cctcgaaccc cgtgagctcg gccgccccgc ccaagagctt gatcatcgac
     2581 tcgtccaagt tgacctcgac gctcggctct ccccgaagag gaatgccctc gaacgagaag
     2641 acaccttgcg cgctcgcgct cgaggcgagc agcatcaagc tgccgaacaa acccatcctg
     2701 ccgttcatat cattagcctc cagtctcgtg tgtatctttc gcgagcgcct cgcggctcgc
     2761 ggcgaacgca tcgcgcaagc cggtgccgac ggcgctctcg acatgtcctt gggtgatgac
     2821 cgcgctcttt tgcaagtagc gcatcgccgt ctcgagatct tgcgcggccg cgacgacgcg
     2881 cggatcgggc ggcggctgcg taggctgcag attcagccac agcacggcgg cgatgacggc
     2941 cgacgccacc gccgcgctcg cgagcgccgg cacgaaatac gagcgccgcg cgcgcggtgc
     3001 ttggctcgga atcgcgagca agcggctgcg caggccgcgc ggcattgaaa ccgccggcgc
     3061 cgcgcgcagt gcgcggtgca ccccgacggc gcgctcggcc gcggcgcgaa gacgcgcatc
     3121 ggccgcgatc gcggcgtcca tgcgcgcgcg gctttcaccc gtcaagctac cgtcggccca
     3181 cgcttcgagc tgggtccacg cccactcttc gttcaaatct ttttgcgtca tgacgcacgc
     3241 tccaattgct cgcgcagctt ccttcgcgcg cgatgcaaat acactttgac ctggttcgca
     3301 ttgagcccga gcacgcgcgc gcacgccgcg ccatccattc cgtgcacgtc gaacatcacg
     3361 agcaagctgc gctgcggctc gggcagcacg tcgacgagct tgcgcagctc gctcgcgagc
     3421 tcgctgtgct cgtaatgcca cgccggatcg cggccgtcgc tgatctcggg caattccgcg
     3481 ggatcttggg tgatgacgcg gccggctctg cgaaagcggc tcaggcacgc attgcgcgct
     3541 acgcgcagca gccactcgcg tggtcgctcg acgttctcgc catgctgcca gtagctcaag
     3601 aaaacctcct gcgtcacgtc ctcggcctcg cgcgaatcgc gcagcagcgc cgaggccagg
     3661 cggaacacgg cgcggtcgtg catcaacacc gcgagccgaa accgccaagt tgacatcacg
     3721 ccgccgcctt cgcgcggctc tgcgtttgcc aactcttccg gttgtggaat cggttgcggc
     3781 gccataccct ttgccgttgg accacgacct tgaagacggc ccaactcagc gaaggttaca
     3841 ccgcaaagga ttgcttcgat gaacggctca cgcgccctag tcctggctct gctagcggtc
     3901 gccggcagtg ccgcggcaaa ccacgacgag agcgtcggtg cacgcttcgt gcagcccggc
     3961 ggcatcgacg ccggcacctg cctcgatcac catgcgccgt gcgtctcgat ccaatacgcg
     4021 ctcgcgcagg cggagcccgg caacacggtc aaggtcggcg cgggtatttt cgacctgagc
     4081 ggcgtggatc ccgagacgtt cgtgcacggg cccgtgcacg cgacgggcgg ctacggcgac
     4141 gccgatcact acttcgaatc gcggccgctt caggtgcagt cgatcatcgt cggcgtcgac
     4201 gctcgctatc gcaacgccct cgccgcgcgc ggcttctact gggccgagag cgtagccgcc
     4261 gcgaagcgca acgagatttc gttcgccgcc ggtacggcac ttcagcagac gcaggccgcc
     4321 gcggcgcagt gcgtgcaggg gatggccggg cagttcccgt gtcgcaatct cgattttcaa
     4381 tcgcaggtcg cgctcgcgca gttctcgagt cggcccgtct ccgccgcg
//
LOCUS       DASSNN010000760         2453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_871529, whole genome shotgun sequence.
ACCESSION   DASSNN010000760 DASSNN010000000
VERSION     DASSNN010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871529"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..658
                     /locus_tag="VFL84_14300"
     CDS             224..658
                     /locus_tag="VFL84_14300"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609848.1"
                     /translation="MTKRIATVVSLLGVGLGLVRPLAAHHSFGAEYDVQQPITLTGVL
                     TKIEWTNPHTHIYLDVADERGAVVNWQLEGYPPVALHRIGWKQNVTMKVGDKITVSGW
                     RARDGGAWGHSREITLPNGEKMMFGPPAGTGDGGGSPAVDVR"
     gene            658..1479
                     /locus_tag="VFL84_14305"
     CDS             658..1479
                     /locus_tag="VFL84_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609849.1"
                     /translation="MRALVAVVLVTAAWAAPALAQAPRADGVPRTAQGKPDLTGVWQG
                     GSTLPGAWDEANAGTGVGGSGRDPNAAVVGSSNDRPAGREGAPYQEWAAKKVLEAYNR
                     RGVDDPTGKCLPAGIPRSVMLGLFPQQFVQTPTQLVILYEYMSTYRVIPFGTQHPDDL
                     IPSYMGNSVAHWDGDTLVVDVVGFNDVTWLAGTGTFHSDALHIVERYTRTSKDRIDYE
                     VTMEDPNVLTKPWVLRSSLMLREGTRLQEYVCAENNLDLERYEQLLRNGVDFTRK"
     gene            1476..1976
                     /locus_tag="VFL84_14310"
     CDS             1476..1976
                     /locus_tag="VFL84_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609850.1"
                     /translation="MRTLASTLVEEVWRQHFVAYEDALAAFYALQDKTEMTLEQSYSI
                     TGEIDRLEALRRTLHDARSQFKASADDGRVVEIERPRAALELDGVRFEVLDLAANRVP
                     PNGALLELGFRVDPLAIPRFVKLTTPIKIKFPFGESTYTASCRLVRFRMIRNQFVFRF
                     ATVDPI"
     gene            2095..>2453
                     /locus_tag="VFL84_14315"
     CDS             2095..>2453
                     /locus_tag="VFL84_14315"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET7609851.1"
                     /translation="MAVGAALPAMTQEPQAGSAEEIIVTGSRVSRSGFDSSQPLTTID
                     SQQIENLGLVNVGDVVRTLPQNTPFFTETNVGIGNFNVGAQLANLRGLNPFFGTRTLT
                     LVDTRRVVPTTEGGAVDL"
BASE COUNT          446 a          777 c          824 g          406 t
ORIGIN      
        1 tgcgcggaga ataccgcgaa agcatgcgta gacgaaaata atatcggcga accacccggt
       61 tcggttgaag cggcgcggcc actggaaccc gcggcatgcg cggttttgat gcctttgtcg
      121 cgcgagtgtg gccaaaatgc cgccgatcga aaattcgcgg cgaagcgcgc gcgggttttt
      181 ggggagtatg atttcgaggc gctaccgcat gggggatggg gcagtgacga agcggatcgc
      241 gactgtcgtc tcgttgctcg gtgtcggctt gggtctagtg cgaccgctcg cagcgcacca
      301 ctcgttcggc gccgagtacg acgtccagca accgatcacg ttgacgggcg tgctgacgaa
      361 gatcgagtgg acgaacccgc acacgcacat ttatctcgac gtcgccgacg agcgcggcgc
      421 cgtcgtcaat tggcagctcg aaggctaccc gcccgtggcg ctgcaccgca tcggctggaa
      481 gcagaacgtc acgatgaagg tcggcgacaa gatcacggtg tcgggctggc gagcgcgcga
      541 cggcggagcc tggggccatt cgcgcgagat cacgctgccg aacggcgaga agatgatgtt
      601 cggcccgccg gccggcacgg gtgacggcgg cggcagcccg gccgtggacg tccgctgatg
      661 cgcgcgctcg tcgcagtcgt gctcgtcacg gccgcctggg cggcgccggc tctcgcgcaa
      721 gcgccgcgcg ccgacggcgt gccgcgcacg gcgcaaggca agcccgacct caccggcgtc
      781 tggcaaggcg gcagcacgct tcccggcgcc tgggacgaag cgaacgccgg cacgggcgtc
      841 ggcggatcgg gccgcgatcc gaacgcggcc gtcgtcgggt cgtcgaacga ccggccggcc
      901 ggccgcgagg gcgctccgta tcaagagtgg gccgcgaaga aggtgctcga ggcgtacaac
      961 cgccgcggcg tcgacgaccc gaccggcaag tgcttgccgg ccggcattcc gcgcagcgtc
     1021 atgctggggt tgtttccgca gcagttcgtg cagacgccga cgcagctcgt gatcctgtac
     1081 gagtacatga gcacgtatcg cgtgattccg ttcggcacgc agcacccgga tgacctcatc
     1141 ccgagctaca tgggcaactc ggtcgcgcat tgggacggcg acacgctcgt cgtcgacgtc
     1201 gtcggcttca acgacgtgac ctggctcgcg ggcacgggca cgtttcactc ggacgcgctg
     1261 cacatcgtcg agcgctatac gcgaaccagc aaggaccgca tcgactacga ggttacgatg
     1321 gaagatccga acgtgctgac gaagccgtgg gtcttgcgtt cgagcctcat gctgcgcgaa
     1381 ggtacgcgac tacaagagta cgtatgcgcc gagaacaacc ttgacctcga gcgctacgag
     1441 cagttgttgc gcaacggcgt cgatttcacg cgtaaatgag aacgctcgcg agcacactcg
     1501 tcgaagaagt ctggaggcag cacttcgttg cctatgagga cgccctcgca gcgttctacg
     1561 cgttgcaaga caagaccgaa atgacgctcg agcagagcta ttcgatcacg ggggaaatcg
     1621 atcggctcga ggcgctgcgc cgcacgcttc acgacgcgcg cagtcagttc aaagcgtcgg
     1681 ccgacgacgg ccgcgtcgtc gagatcgagc ggccgcgcgc cgcgctcgag ctcgacggcg
     1741 tccgtttcga ggtcctggat ctcgcggcca atcgcgtgcc gccgaacggc gcgctgctcg
     1801 agctcggctt ccgcgtcgac ccgctggcga tccctcggtt cgtcaagctg acgacgccga
     1861 tcaagatcaa atttccgttc ggtgaatcga cctacacggc gtcgtgccgg ctggtgcggt
     1921 tcagaatgat tcgcaaccaa ttcgtgtttc gctttgcgac ggtcgatccg atctagaggg
     1981 cgcaacgccg aatttttcaa taaccattaa cagggacggg aatcatccat gacgacttct
     2041 cgagtgaatg tgcgatacca agcgtcgcgt cttggtgcgt tgagcgcgct tgcggtggcc
     2101 gtcggggccg cgttgccggc gatgacgcaa gagccgcagg ccggctcggc tgaagaaatc
     2161 atcgtcacag gctcgcgcgt gagccgcagc ggcttcgact cgtcgcagcc gctcacgacg
     2221 atcgactcgc agcagatcga gaacttgggg ctcgtcaacg tcggcgacgt cgtgcgcacg
     2281 ctgccgcaga acacgccgtt cttcaccgag acgaacgtcg gcatcggcaa cttcaacgtc
     2341 ggcgcgcagc tcgcgaactt gcgcggcctg aacccgttct tcggcacgcg gacgctgacg
     2401 ctcgtcgaca ctcggcgtgt cgtgccgacg acggaaggcg gcgccgtcga cct
//
LOCUS       DASSNN010000761         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_995393, whole genome shotgun sequence.
ACCESSION   DASSNN010000761 DASSNN010000000
VERSION     DASSNN010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995393"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFL84_14320"
     CDS             <1..807
                     /locus_tag="VFL84_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609852.1"
                     /translation="RILAAVLALLTAAAAHAQVRTEIPRAANGRPDISGVWQALNTAH
                     WNLEPHVSEYPVLLELGAQFAVPPGAGVVVGGEIPYLPEARAERDRRFENRLIDDPEG
                     KCYMGGVPRSTYMPYPFQILQNEHDVVIYYQFATAFRRIFVDGKEEAPLDSWMGWSNG
                     HWDGNTFVVEVTGLNGLTWLDRAGNYASGNAKITERYTPLGPNHLQYEATIDDSTVFS
                     RPWTIRMPLYRIVDEGFRILEFKCEPYAEEKIYGHLRKPGTEPPAGSSSR"
     gene            804..1760
                     /locus_tag="VFL84_14325"
     CDS             804..1760
                     /locus_tag="VFL84_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609853.1"
                     /translation="MRYRTALAVAGLAIAIAPLCAHAQKKPRTAWGDPDITGAYSEFT
                     TAPLERDPKYAEKEFLAREEYEAFAKARLETRFGDDDTEIGTQADVHYSMGQFQLSEN
                     EGVHSLNLRTSIVTQPKNGRIPPLTAEAEARRDARAAARRGKEFDGPEMRSLTERCII
                     WSSTQAPILPRGYNSNLQIFQSPGYVIIQGEMGDPRVIPTDGRKLPDKLLPQWNGTSV
                     GRFEGDTFVVETAGFHPESAWRNASENMHVTERITRVDDDTLEYAFTVVDPDTWAEPW
                     GGVYPLTKLDGLLYEYACTEGNYGMANILSGARAGEKATAAK"
     gene            complement(1802..>2279)
                     /locus_tag="VFL84_14330"
     CDS             complement(1802..>2279)
                     /locus_tag="VFL84_14330"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7609854.1"
                     /translation="EGYVFSESLTLHGPYAGRGPKGYKRSDQQIVEDACQRLERDGEV
                     DASDIEVTAELGVIRLRGTVPDRRTKRRAEECVESVYGAHDVMNELRVSYEGREQWQG
                     GQSSQSQGAQAGRGTQASQASQGTQSSQGTQTSGQRPGAGTATSDQLGEDKRSPRH"
BASE COUNT          397 a          782 c          747 g          353 t
ORIGIN      
        1 cgcatcttgg cagccgtgct cgcgctgctc accgcagcgg ccgcgcatgc tcaagttcga
       61 accgagattc cgcgcgccgc gaacggccgc cccgacatct cgggcgtctg gcaagcgctg
      121 aacaccgcgc actggaacct cgagccgcac gtctcggaat atcccgtcct gctcgagctc
      181 ggcgcgcagt tcgcggtgcc gcccggcgcc ggcgtcgtcg tgggcggcga gatcccgtat
      241 ttgccggaag cgcgcgccga gcgcgatcgc cgcttcgaga atcgcttgat cgacgacccg
      301 gaaggcaagt gctacatggg cggcgtgccg cgctcgacgt acatgcccta cccgttccag
      361 atcctccaga acgagcacga cgtcgtcatc tactatcagt tcgcgacggc gttccgccgc
      421 atcttcgtcg acggcaagga agaagcgccg ctcgacagct ggatgggctg gtcgaacggg
      481 cattgggacg gcaacacgtt cgtcgtcgag gtcaccggct tgaacggcct cacgtggctc
      541 gaccgcgccg gcaattacgc gagcggcaac gcgaagatca ccgagcgcta cacgccgctc
      601 gggccgaacc acttgcagta cgaggcgacg atcgacgact cgaccgtctt ctcgcggccg
      661 tggacgatcc gcatgccgct ctatcgcatc gtcgacgaag gcttccgcat cctcgagttc
      721 aagtgcgagc cgtatgcgga agagaagatc tacgggcatt tgcgcaagcc gggaaccgag
      781 cctccggcag ggagctcaag ccgatgagat accgaacagc tctggccgtt gcgggattgg
      841 ccatcgcgat cgcgccgctc tgcgcgcacg cgcagaaaaa gccgcgcacc gcgtggggcg
      901 atcccgacat cacgggagcg tattccgagt tcacgacggc gccgctcgag cgcgacccca
      961 agtacgccga gaaggaattc ctcgcgcgcg aggagtacga ggcgttcgcg aaggcgcgtc
     1021 tcgagacgcg attcggcgac gacgatacgg agatcggcac tcaggccgac gtgcactaca
     1081 gcatgggcca gttccagctg agcgagaacg aaggcgtgca ctcgctgaat ctgcgcacgt
     1141 cgatcgtgac gcagccgaag aacggccgca tcccgccgct gacggccgaa gcggaggcgc
     1201 gccgcgacgc gcgcgccgct gcgcgcaggg gcaaagagtt cgacggcccc gagatgcgct
     1261 cgctgacgga gcgctgcatc atctggtcga gcacgcaagc gccgatactg ccgcgcggct
     1321 acaacagcaa cctgcagatt tttcagagcc cgggctacgt gatcatccaa ggcgagatgg
     1381 gcgatccgcg cgtgatcccg acggacgggc gcaagctccc cgacaagctc ctgccgcagt
     1441 ggaacggcac gtcggtcggg cgctttgagg gcgacacgtt cgtcgtcgag acggcgggct
     1501 tccatcccga gagcgcctgg cgcaacgcga gcgagaacat gcacgtcacg gagcgcatca
     1561 cgcgcgtcga cgacgacacg ctcgagtacg cgttcacggt cgtcgacccc gacacgtggg
     1621 ccgagccgtg gggcggcgtc tatccgctca cgaagctcga cggcttgctc tacgagtacg
     1681 cctgcacgga aggcaactac ggcatggcga acatcctctc gggcgcccgc gccggcgaga
     1741 aggcaaccgc cgccaaatga catcatgccg gcgcgggatg accgcgccgg cgtgagctct
     1801 gctagtgccg cggcgagcgt ttgtcctcgc ctagttggtc ggacgtcgcc gtgccggcgc
     1861 ccggccgctg acccgatgtc tgcgttcctt gcgacgactg cgtgccctgc gatgcttgcg
     1921 acgcttgcgt gccgcggccg gcttgcgcgc cctggctttg cgagctctgc ccgccttgcc
     1981 actgctcgcg cccttcatac gacacacgca gctcgttcat gacgtcgtga gcgccgtaga
     2041 ccgattcgac gcactcctcg gcgcggcgtt tggtcctgcg gtcgggaacc gtgccgcgca
     2101 ggcggatcac gccgagctct gcagtgacct cgatgtcgct cgcgtcgacc tcgccgtcgc
     2161 gctcgagacg ctgacacgcg tcctcgacga tctgttggtc cgagcgcttg tagcccttcg
     2221 gcccgcggcc cgcataaggg ccgtgcagcg tcaacgactc ggagaagacg tagccttca
//
LOCUS       DASSNN010000762         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1880515, whole genome shotgun sequence.
ACCESSION   DASSNN010000762 DASSNN010000000
VERSION     DASSNN010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880515"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFL84_14335"
     CDS             <1..205
                     /locus_tag="VFL84_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009470989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HET7609855.1"
                     /translation="DFPIIPCNLCGSQENMKRREIKAMLASWEREDPGRTERLFRSLQ
                     NVTPSHLADRELFDFESLAGADD"
     gene            complement(202..1503)
                     /locus_tag="VFL84_14340"
     CDS             complement(202..1503)
                     /locus_tag="VFL84_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7609856.1"
                     /translation="MEPSADNAPRILILEPDIKVRSSLLRYAIKGWQGAAVQSTSGTL
                     TEALADPERLKNFDVLLVGCDFSQDGTAENPTLQAIRAVAAEPRNPAIILLTTKGSEY
                     TAVQAIKSGAFDYIPKTLLGREQVLSAVQRAMLHRKGLLGTRGGTVTGVVRLFGSDMR
                     RCLATRDSVSVHVAFSAERAKEVVLKVLHRGRGSLSRDANFERLVMEFKLLYDINDHA
                     VAEIYDFRVTSQYCYIAMEYFPLGHLGMQLDRNVSPHEALYYAREIARALSIIHMAGV
                     VHRDLKPANIMLRDDRSIALIDFGISQSTHTAKAEPQGTTREIAGTPYYMSPEQAAGL
                     AADERTDLYSLGVILYQMLTGDKPYVGTTADEILEQHRNAALPILPSELSSCQPLLNK
                     LLAKEPGQRFGSAREALEALQHMMGTLAGAAQPSAASAAAS"
     gene            complement(1584..>2147)
                     /locus_tag="VFL84_14345"
     CDS             complement(1584..>2147)
                     /locus_tag="VFL84_14345"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7609857.1"
                     /translation="LALFCCTFAAAAQPNLDWIDRDAIAAREVQIVVERSNRPLTAEV
                     KVASEVDAPATAIWDVLKACEIAPEYVPNVVSCRKVEELDGGRADLFMQTIKPIFFVP
                     AFEHEFRLDYTPYTRIDVNRVSGPIAHMQGTWWLLPEDNGRILLVYELTLDPGMPIPR
                     FFVRATLKHDLPRLVAAIRERAEAAAP"
BASE COUNT          352 a          694 c          731 g          370 t
ORIGIN      
        1 cgactttccg atcattccgt gcaacctctg cggctcgcag gagaacatga agcggcgcga
       61 gatcaaagcg atgctcgcga gctgggagcg cgaggacccg ggtaggaccg aacggttgtt
      121 ccgcagcctc cagaacgtga cgccgtctca tctcgccgac cgcgagctgt tcgacttcga
      181 gagcctggcg ggcgccgacg actagctcgc cgccgcggag gccgcgctcg gctgcgccgc
      241 gcccgccaac gtacccatca tgtgctgcag cgcctcgagc gcttcacgag cgctgccgaa
      301 gcgctgaccc ggttctttcg cgagcagctt gttcaagagc ggctgacacg aggagagctc
      361 ggacgggaga atcggcagcg ccgcattgcg gtgttgctcc aggatttcgt cggcggtcgt
      421 gccgacgtag ggtttgtcgc ccgtcaacat ctgatacagg atcacgccga gcgaatagag
      481 atccgtgcgc tcgtcggcgg cgagcccggc cgcttgctca gggctcatgt agtaaggcgt
      541 tcccgctatc tcgcgcgtcg tgccctgcgg ctccgccttc gccgtgtgcg tcgactgcga
      601 gatgccgaag tcgatcaacg cgatgctgcg gtcgtcgcgc agcatgatgt tggcgggctt
      661 caaatcgcgg tgaacgacgc cggccatgtg gatgatcgac agcgcgcgcg cgatctcgcg
      721 cgcgtagtag agcgcttcgt gcggcgacac gttgcgatcg agctgcatgc ccaagtgtcc
      781 gagcggaaag tattccatcg cgatgtagca gtactggctc gtgacgcgaa agtcgtagat
      841 ctcggcgacg gcgtggtcgt tgatgtcgta gagcagcttg aactccatga cgaggcgctc
      901 gaaattcgcg tcgcgcgaca gcgagccgcg gccgcgatgc agcaccttga gcacgacctc
      961 cttggcgcgc tccgcgctga acgctacgtg aaccgagacg ctgtcacgcg tcgcgaggca
     1021 gcggcgcatg tcggagccga acagccggac gacgcccgtc acggtgcctc cgcgtgtgcc
     1081 gagcaacccc ttgcgatgca gcatcgcgcg ctgcacggcc gagagcacct gctcacggcc
     1141 gagcagcgtc ttcgggatgt agtcgaacgc gcccgacttg atcgcctgga cggccgtgta
     1201 ctcgctgccc ttcgtcgtca gcaggatgat ggccgggttg cgcggctcgg cggccacggc
     1261 gcggatcgcc tgcagcgtcg ggttctcggc agtgccgtcc tgcgaaaagt cgcagcccac
     1321 gagcagcacg tcgaaattct tgagccgctc cggatcggcg agcgcctcgg tcagcgtgcc
     1381 cgacgtcgac tggaccgccg cgccttgcca gcctttgatc gcgtaacgca gcagcgaaga
     1441 gcggaccttg atgtccggct ccagaatcag gattcgcggc gcgttgtcgg cggacggctc
     1501 cacagctcgg gaaccgtacc aaatccgcaa ctgtggggcg gtgacgctcg tctcgctgcc
     1561 ggctcggcga tccgccgaat ccgtcaaggc gcggcggctt cggcgcgctc ccggatggcc
     1621 gcgacgagcc tcggcaggtc gtgtttcagc gtggcgcgga cgaagaagcg cggaatcggc
     1681 atcccggggt cgagcgtaag ctcgtagacg agcaggatgc ggccgttgtc ctcgggcaga
     1741 agccaccagg ttccttgcat gtgtgcgatc ggcccgctga cgcggttgac gtcgatccgc
     1801 gtgtacggcg tgtagtcgag ccgaaactcg tgctcgaatg ccggcacgaa gaagatcggc
     1861 ttgatggtct gcatgaacag gtcggcgcgg ccgccgtcga gctcctcgac cttccggcag
     1921 ctcacgacgt tcgggacgta ctcgggcgcg atctcgcacg ccttgagcac gtcccagatc
     1981 gccgtcgcgg gagcgtcgac ctcggacgcg accttcactt ccgccgtgag cggccggttg
     2041 ctgcgctcga ctacgatttg gacttcccgc gccgcgatcg catcgcggtc gatccagtcg
     2101 agattgggtt gcgccgccgc ggcgaaggtg cagcagaaga gcgcgag
//
LOCUS       DASSNN010000763         4183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_367929, whole genome shotgun sequence.
ACCESSION   DASSNN010000763 DASSNN010000000
VERSION     DASSNN010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367929"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="VFL84_14350"
     CDS             <1..1113
                     /locus_tag="VFL84_14350"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609858.1"
                     /translation="DWLAAGVVMVWRVLGGLLVGCGMAVPAHAHHSFAAEFEADKTAE
                     LSGKIVQVWWNNPHVRYRLQVAGEGGATEDWELQAASITAMSQLGWTQATIEVGDQLT
                     VSGQVGRNGTKKLYVRNIVRPDGTRLATGRGDTAGPDPNQVHATAGKTYGYGSTRNAY
                     PADISGPWRNRYKWRLTVDDLEPKPTPFTAEGRAAFEATDHYQDPALRCLALGLPRLF
                     GAPYNMEIVDAGTHYVFIYVEHNTPRRVFMDGRKPPPDTAPSSLGFSVGHWEGNALVI
                     ETTHLLPAWLDGSGLPMKGEGTRIVERYELAPDKLAMDRIMTIYDPYYTQPLVRRRGS
                     ARDDNVDIAEQASCDPDSYYRDLLELGKLEQHFDGR"
     gene            complement(1110..1643)
                     /locus_tag="VFL84_14355"
     CDS             complement(1110..1643)
                     /locus_tag="VFL84_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609859.1"
                     /translation="MLPGAAAAAALAIFALAAQFSPQRAAAQSTPCTAIADDAERLAC
                     YDRALRSAAPEPPAPRTPAPAAPPPQTPSAPAAEPPAPNAAQAPAARQSPQPQDQDAD
                     KQTVPLVIVGVRTLPGRETLFTAQDGTVWVQTDSQRIVGLPATPFDAELKPGAMSSQF
                     LVPKNGARAIRVRPVER"
     gene            1794..3767
                     /locus_tag="VFL84_14360"
     CDS             1794..3767
                     /locus_tag="VFL84_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005807847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET7609860.1"
                     /translation="MAEPTPEAAAEFRVEVERFADVRILRYRVPGFEALDARTKRLLY
                     YLYEAALCGREITYDQKYRYNLSVKRTLEQIVRHYAGDRDTADFRALTLYLKRVWFSN
                     GIHHHYAHDKFRPEFSAAAFAAFVRGTPAQFPLREGQSLEQLIDELARVMFDPALDAK
                     LVSKSADRDVLTSSAVNFYAGLTQLEVEAFYAGAAGETKVSLGLNSRLVKVDGAAVEQ
                     VQKVGGRYTEAIEHIVAWLTKAAGCAENDAQRSALEKLIRFYRTGSLEDWDEYNIAWL
                     RDTESHVDVVNGFIEVYNDPLGMRGTFESIVSFRDTEATRRIGTIAAAAQWFEDHMPF
                     FGRHKKPSVTGISGKVITIVVESGDAAPSTPIGVNLPNSDWIRREHGSKSVTLANIVA
                     AYNAVRGGVDREFCYSAAEAERNDRYGELAATLHVDLHEVIGHGSGQIEQGVRPLHET
                     LKNYGSTLEEARADLVALYFAVDPKLVELGLQPHVEVGHAAYDQFIRNALLQQLNRVE
                     PGKELEEDHMRNRQLIAAWAYEHGLADNVIERRSRDGKTFFVINDYAKLRTLFGRLLR
                     ELQRIKSTGDYAAIRELVERYAVKADPALCAEVRARYAALDVPAYTGFIGPRLVPLRN
                     GDEIVDVRLEYPDDFAAQMLEYADRYAFLPTWN"
     gene            3778..>4183
                     /locus_tag="VFL84_14365"
     CDS             3778..>4183
                     /locus_tag="VFL84_14365"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009575820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HET7609861.1"
                     /translation="MNRLGIAACLLALAPHGASSAQEAAGAAPAPKVVLIIGDGMDQQ
                     QITIARDYIAGYDGRLTLDTLPVRSSVQIQTVAEEDPSMPEYVADSANTATSIATGIV
                     TSAGRISTTAGTDLDAPTIMEIAAAAGVGTGIV"
BASE COUNT          708 a         1405 c         1406 g          664 t
ORIGIN      
        1 gactggctcg cggcgggggt cgtcatggtg tggcgcgttt tgggtgggtt gctcgtgggt
       61 tgcggtatgg ccgtgccggc gcatgcccac cactcgttcg cggccgagtt cgaagccgac
      121 aagaccgccg agctcagcgg caagatcgtc caagtctggt ggaacaaccc gcacgtccgc
      181 tatcggctgc aggtcgccgg cgaaggcggt gcgaccgagg actgggagct gcaagccgcg
      241 agcatcacgg ccatgtcgca gctcggctgg acgcaagcga cgatcgaggt cggcgatcag
      301 ctcacggtct cgggacaggt cggacgcaac gggacgaaga agctctacgt tcgcaacatc
      361 gtgcggccgg acggcacgcg gctcgcgacg ggccgcggcg acaccgcggg gcccgatccg
      421 aatcaagtcc acgcgaccgc gggcaagacc tatggctacg gctcgacacg caatgcgtat
      481 cccgccgaca tctcgggtcc gtggcgcaat cggtacaagt ggcgcctgac cgtcgacgac
      541 ctcgagccga agccgactcc gttcacggcc gaagggcgcg cggcgttcga agcgacggat
      601 cactatcaag atcccgcgct tcgctgcctc gcgctcggtc taccgagact gttcggcgcg
      661 ccctacaaca tggagatcgt cgacgccggc actcactacg tcttcatcta cgtcgagcac
      721 aacacgccgc gacgagtctt catggacggc cgcaagccgc ctcccgatac cgcgccgtcg
      781 tcgctcggct tctcggtcgg acattgggaa gggaacgcgc tcgtcatcga gacgacgcac
      841 ttgttgcccg cttggctcga cggttcgggt ctgccgatga agggcgaagg cacgcgcatc
      901 gtcgagcggt acgagctcgc gcccgacaag ctcgcgatgg accgcatcat gacgatctac
      961 gatccgtact acacgcagcc gctcgtgcgc cggcgcggct cggcgcgcga cgacaacgtc
     1021 gacatcgccg agcaggcgag ctgcgatccc gacagctact accgcgactt gctcgagctc
     1081 ggcaagctcg agcagcactt cgacggccgc tagcgttcga cgggccggac gcgaatcgcg
     1141 cgcgcgccgt tcttcggcac gagaaactgg ctgctcattg cgcccggctt gagctcggcg
     1201 tcgaacggcg tcgcgggaag accgacgata cgctggctat ccgtctgcac ccagaccgtg
     1261 ccgtcctgcg ccgtgaacag cgtctcgcgt cccggcaacg tgcgcacgcc gacgatgacc
     1321 aatggcaccg tctgcttgtc cgcgtcttgg tcttgcggct gcggcgactg tcgcgccgcg
     1381 ggagcttgcg cggcattcgg cgccggcggc tcggcagccg gagcgctcgg cgtttgcgga
     1441 ggcggcgcgg ccggcgccgg tgtcctcggc gcgggcggtt ccggcgcggc tgaacgcagc
     1501 gcgcggtcat agcaagcgag acgctcggcg tcgtccgcga tcgccgtgca cggtgtgctt
     1561 tgcgccgcgg cccgttgcgg cgagaactgc gccgccagcg cgaaaatggc gagggcggcg
     1621 gctgctgccg cgccgggaag caatgtgtca tgatcccgac cctcacgcgg agccgcggtc
     1681 ctcattccga tcgtcccccg attacagcaa tgtccgagac aacgtccgcc atagtggccg
     1741 cttcaggcgc ggccgatcaa ggggccgagc cgcctgcacg ggctcgcgta ggcgtggccg
     1801 agcctacgcc cgaggcggcc gcggagtttc gcgtagaggt cgagcgcttc gccgacgtgc
     1861 ggatcttgcg ctaccgcgtg ccgggcttcg aggcgctcga cgcacgtacg aaacggctcc
     1921 tttattatct gtacgaggcg gcgctctgcg gccgcgagat cacctacgat cagaagtacc
     1981 gctacaactt gagcgtaaag cgcacgctcg agcaaatcgt ccggcactat gcgggcgacc
     2041 gcgacaccgc cgattttcgc gcgctgacgc tttatttgaa gcgcgtctgg ttctcgaacg
     2101 gcattcatca tcactacgcg cacgacaagt tccggcctga gttttcggcc gcggcgttcg
     2161 cggcgttcgt gcgcggcacg ccggcgcagt ttccgctgcg cgagggccag tcgctcgagc
     2221 agctcatcga cgagctcgcg cgcgtcatgt tcgatccggc tctcgacgcg aagctggtca
     2281 gcaagagcgc cgatcgcgac gtgctgacga gctctgccgt caatttctac gccgggctca
     2341 cgcagctaga ggtcgaagcg ttctatgcgg gcgcggccgg cgagacgaag gtctcgctcg
     2401 gcctcaattc gcggctcgtc aaggtcgacg gcgccgccgt cgagcaggtc cagaaagtcg
     2461 gtgggcgcta cacggaggcc atcgagcaca tcgtcgcctg gctcacgaag gcggcgggct
     2521 gcgccgagaa cgatgcgcaa cgctctgcgc tcgagaagct gattcgcttc taccgaaccg
     2581 gcagcctcga ggattgggac gagtacaaca tcgcttggct gcgcgacacc gagtcgcacg
     2641 tcgacgtcgt caacggtttc atcgaggtct acaacgaccc gctcggcatg cgcggcacgt
     2701 tcgagagcat cgtctcgttc cgcgacacgg aagcgacgcg ccgcatcggc acgatcgccg
     2761 ccgccgcgca gtggttcgag gatcacatgc cgttcttcgg ccggcacaag aagccgagcg
     2821 tgacgggcat ttccggcaag gtcatcacga tcgtcgtcga gtcgggagac gcggcgccgt
     2881 cgacgccgat cggggtcaac ctgccgaact cggattggat ccggcgcgag cacgggtcga
     2941 agtccgtgac cctcgcgaac atcgtcgcgg cgtacaacgc cgtgcgcggc ggcgtcgatc
     3001 gcgagttctg ctactccgcc gccgaggccg agcgcaacga ccgctacggc gagctggccg
     3061 cgacgctgca cgtcgatttg cacgaagtca ttggtcacgg gtcggggcaa atcgagcagg
     3121 gcgtccggcc gctgcacgag accctgaaga actacggctc gacgctcgag gaagcgcgcg
     3181 ccgatctcgt cgcgctgtat ttcgccgtcg atccgaagct cgtcgagctt gggctgcagc
     3241 cgcacgtcga ggtcgggcac gcggcctacg accagttcat ccgcaatgcg cttctgcagc
     3301 agttgaaccg cgtcgagccc ggcaaggagc tcgaggaaga ccacatgcgc aaccgccagc
     3361 tcatcgcggc gtgggcgtat gagcacggcc tcgcggacaa cgtcatcgag cggcgctcgc
     3421 gcgacggcaa gacgttcttc gtgatcaacg actacgcgaa gctgcgcacg ctgttcggcc
     3481 ggctcctgcg cgagctgcag cgcatcaagt ccacgggcga ctacgcggcg attcgcgagc
     3541 tcgtcgagcg ttacgccgtc aaggccgacc ccgcgctctg cgccgaggtg cgcgcgcgtt
     3601 atgcggcgct cgacgtgccg gcctacacgg gcttcatagg gccgcgcctc gtgccgctgc
     3661 gaaacggcga cgagatcgtc gacgttcgtc tcgaataccc cgacgatttc gccgcgcaaa
     3721 tgctcgagta tgcggatcgc tacgctttct tgccgacgtg gaattgagct ctatcgaatg
     3781 aacaggctcg gcatcgccgc gtgtctactg gcgcttgcgc cgcacggcgc ttctagcgct
     3841 caagaggcgg ccggcgcagc gcctgcgccg aaagtcgtgc tgatcatcgg cgacggcatg
     3901 gaccagcagc aaatcacgat cgcgcgcgac tacatcgccg gctacgacgg ccggctcacg
     3961 ctcgacacgc tgccggtgcg cagcagcgtg cagatccaga ccgtcgccga ggaagacccg
     4021 agcatgccgg agtacgtcgc cgattccgcg aacacggcga cgtcgatagc gacgggtatc
     4081 gtgacgagcg cgggccgcat ctcgacgacg gcgggcacgg acttggacgc cccgacgatc
     4141 atggagatcg ccgcggcggc cggcgtcggc accggcatcg tca
//
LOCUS       DASSNN010000764         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_704057, whole genome shotgun sequence.
ACCESSION   DASSNN010000764 DASSNN010000000
VERSION     DASSNN010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704057"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2391)
                     /locus_tag="VFL84_14370"
     CDS             complement(<1..2391)
                     /locus_tag="VFL84_14370"
                     /inference="COORDINATES: protein motif:HMM:NF009586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET7609862.1"
                     /translation="MGTLLWLVLLIGTLLTLAYRRTDLKTATIVLGALLVAYLLFGDG
                     GFLWNLLLIVLFVLFALLNVEQFRRDNVTRRVFEIYRRMLPSMSKTEQEALEAGNVWW
                     DGELFTGMPDWRRLKNLPPPKLSAEEQAFLDGPTEELCRLLDDWQITHELADMPANVW
                     QFLKDHKFFAMIIPKEYGGLEFSPLAMSMVLAKVSSRSAVASSTIGVPNSLGPAELLL
                     HYGTDEQKQYWLPRLASSEEIPCFALTSPRVGSDATALVDNGVVCRGTWQGSEIVGIR
                     LNWDKRYITLAPIATVMGLAFKLRDPDHLIGDVDDYGITAALIPTGLPGIEIGRRHFP
                     IDIPFQNGPIRGRDVFVPLDAIIGGTKMAGQGWHMLVQQLAVGRSIVLPSNALGGAQA
                     ALYATGAYAKVRRQFNLPIGKFHGVGEAIARMAGSVYTMMAASRVTCTAITNGEKPAV
                     PSAILKYHNTEMSRQVASDAMDVHGGKGIMLGPKNYLARGYEGVPIAITVEGANILTR
                     NLIIFGQGAIRCHPFVRAEMDAARNPDTQAGLVAFDRLLFKHLGYGLSNAARSLVMAV
                     TFARFTDVPIAGPTRRYYQHINRYSASFALITDAAMFTLGGELKRRELLSARLGDILS
                     YLYLASMVLKHYQDQGEPPEDLPLVEWSCRTLLYKTQEQLHGLLRNFPNRWVAAVLRF
                     FIFPRGRTYFAPSDVLGQEIVELMINPTPTRERLAAGIYTAVEPHNQLGLLQLAMEIA
                     EQVKPLERRVFDARRAGEIKADDIPSQIDEAEQKGILTPAEAEAVRAFDRRVLDVTG"
BASE COUNT          424 a          826 c          840 g          421 t
ORIGIN      
        1 gcccgtgacg tcgagcacgc gccggtcgaa cgcgcgcacg gcttccgcct cggccggcgt
       61 cagtatgccc ttctgctcgg cctcgtcgat ctggctcgga atgtcgtcgg ccttgatctc
      121 gccggcacgc cgcgcgtcga agacgcgccg ctcgagcggc ttgacctgct cggcgatctc
      181 catcgccagc tgcagcagcc cgagctgatt gtggggctcg acggccgtat agatgccggc
      241 cgcgagccgc tcgcgcgtcg gcgtcgggtt gatcatgagc tcgacgatct cttggccgag
      301 cacatcggac ggcgcgaagt aggtgcggcc gcgcgggaag atgaagaagc gcaagacggc
      361 ggcgacccaa cggttcggga agttgcgcag caggccgtgc agctgctcct gcgtcttgta
      421 cagcagcgtg cggcaagacc actcgacgag cggcagatcc tcgggcggct cgccttggtc
      481 ctgatagtgc ttgagcacca tcgacgccaa gtacaaatag ctcaagatgt cgccgagccg
      541 cgccgacagc agctcgcgcc gcttgagctc gccgccgagc gtgaacatcg ccgcgtccgt
      601 gatcagcgcg aacgaggcgc tgtagcggtt gatgtgctgg taatagcggc gcgtcgggcc
      661 ggcgatcggc acgtcggtga agcgcgcgaa cgtgaccgcc atgacgagcg agcgtgccgc
      721 gttcgagagg ccgtacccca agtgcttgaa caacagccgg tcgaaggcga cgagaccggc
      781 ttgcgtgtcg ggattgcgcg cggcgtccat ctccgcgcgc acgaacggat gacagcgaat
      841 cgcgccctga ccgaagatga tcaagttgcg cgtcaagatg ttcgcgcctt cgaccgtgat
      901 cgcgatcggc acgccttcat agccgcgcgc gaggtaattc ttcggcccga gcatgatgcc
      961 cttgccgccg tgcacgtcca tcgcatcgct cgcgacttgc cggctcatct cggtgttgtg
     1021 gtacttcaag atcgccgacg gcacggccgg cttctcgccg ttcgtgatcg ccgtgcacgt
     1081 cacgcgcgac gcggccatca tcgtgtagac gctgccggcc atgcgcgcga tcgcctcgcc
     1141 gacgccgtga aacttgccga tcggcaggtt gaactggcgg cgcactttcg cgtacgcgcc
     1201 cgtcgcgtaa agcgcggcct gcgcgccgcc gagcgcgttg ctcggcagaa cgatgctgcg
     1261 tccgaccgcg agctgctgca cgagcatgtg ccagccttgg ccggccatct tcgttccgcc
     1321 gatgatcgcg tcgagcggca cgaacacgtc gcggccgcgg ataggaccgt tctggaacgg
     1381 aatgtcgatc gggaaatggc gccggccgat ctcgatgccg ggcagccccg tcgggatcag
     1441 cgcggccgtg atgccgtagt cgtcgacgtc gcctatcaga tggtccggat cgcggagctt
     1501 gaacgcgagg cccatgacgg tcgcgatcgg cgcgagcgtg atgtagcgct tatcccagtt
     1561 caggcgaatg ccgacgatct cgctgccttg ccacgtgccc ctgcagacga cgccgttgtc
     1621 gacgagcgcc gtcgcgtcgg agccgacgcg cggcgacgtc agcgcgaagc acggtatctc
     1681 ttcgctgctc gcgagccgcg gcagccagta ctgcttctgc tcgtcggtgc cgtagtgcag
     1741 cagcagctcg gcggggccga gcgaattcgg cacgccgatc gtcgacgacg cgacggcgct
     1801 gcgcgaggac actttcgcga ggaccatcga catcgcgagc ggcgagaact cgaggccgcc
     1861 gtactccttc ggaatgatca tcgcgaagaa cttgtggtcc ttcaggaact gccagacgtt
     1921 cgccggcatg tcggcgagct cgtgcgtgat ctgccagtcg tcgagcaggc gacagagctc
     1981 ctcggtcggt ccgtcgagga acgcttgctc ctcggccgag agcttcggcg gcggcaggtt
     2041 cttgagccgg cgccaatccg gcatgcccgt gaagagctcg ccgtcccacc agacgttgcc
     2101 ggcttcgagc gcttcctgct ccgtcttgga catgctcggg agcatgcgcc ggtagatctc
     2161 gaacacgcgc cgcgtgacgt tgtcgcggcg aaactgctcg acgttcagca acgcgaacag
     2221 cacgaacagc acgatcagca gcaggttcca gagaaagccg ccgtcgccga acagcagata
     2281 ggcgacgagc aacgcgccga gaacgatcgt cgcggtcttc aagtccgtgc ggcgataggc
     2341 gagcgtcagc aacgttccga tcaggagcac gagccagagc agggttccca tcgcgactcc
     2401 ttagcctgga acgagagcga gtacctgcgc gccgcctgaa gaagcgctag agcaggtttg
     2461 cgactccgac gcgctcttcg cggagctcgg cttccgtggc cgccatcttc g
//
LOCUS       DASSNN010000765         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1718040, whole genome shotgun sequence.
ACCESSION   DASSNN010000765 DASSNN010000000
VERSION     DASSNN010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718040"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="VFL84_14375"
     CDS             <1..1464
                     /locus_tag="VFL84_14375"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HET7609863.1"
                     /translation="AACPAGLLWAQDRPTEPFDRAALLAKAAELAKQPHALPPNPQPD
                     GRRLTYEQYRSIRFDPAAAIWRTENRNFAVDLLYPGFIFDVPVNINLVVGGVARRVLF
                     TNERFDYGPEVPSVAATGEYTGYSGFRVRAPLNSPDYLDEFLVFQGASYFRAVAKGQL
                     YGISARGLAVRTARPEGEEFPYFTDFWIERPPEGATEIVIHALLQSPSVVGAYTFKAR
                     PGEETIVDVDAALFPRVDLTAFGIAPLMSMFLFNASNRFRFDDYRAAVHDSDGLQILN
                     GRGERLWRPLANPRMTSQTSAFVDDSPKGFGLLQRKRNFADYQDAEAQYERRPSLWVE
                     PATEWGPGHVELVEIPSPRVNDNIVAYWQPREPILAGQSREFSYRLRFTDEPLDDSLA
                     RVVATRVGQAPNAEGQRAFVIDFKGAGDIPDDLVPEVWSSAGEVFAPRGQVVAQAGTY
                     RVAFELDPKRENLIEMGVVLRSQGKPWSETWLYQWSR"
     gene            1440..>2103
                     /locus_tag="VFL84_14380"
     CDS             1440..>2103
                     /locus_tag="VFL84_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis glucosyltransferase H"
                     /protein_id="HET7609864.1"
                     /translation="MVVPVEPMTLRHSSSVPPFAELDMPEQNLRRYVRERRLARPSFF
                     TVLARLLTFGGALALTVYATLEMIAVVSVGIVSALQWAMVALFAITFAWIALPATAAV
                     SGVLFGWVRRRAKADLRVEQRTALVMPVYNENPAGTFAALLAMAEALTEQNATGFEIF
                     VLSDTTRPEIYVKETAAYHVLREKLGERMRVWYRRRPDNVGRKVGNLHDFVTRWGGRY
                     DFM"
BASE COUNT          322 a          721 c          695 g          365 t
ORIGIN      
        1 gccgcttgcc cggccgggct cttgtgggcc caggaccggc cgacggagcc gttcgatcgc
       61 gcggcgctgc tcgccaaagc ggccgagctg gccaagcagc cgcatgcgtt gccgccgaat
      121 ccgcagcctg acggccgccg cctcacttac gagcaatatc gctcgatccg cttcgatccc
      181 gccgcggcga tctggcgcac cgagaaccgc aatttcgccg tcgatctgct ctaccccggt
      241 ttcatcttcg acgtccccgt caacatcaac ctcgtcgtcg gcggcgtcgc gcgccgtgtg
      301 ctgttcacga acgagcggtt cgactacgga cccgaagtgc cgagcgtcgc cgccaccggc
      361 gagtacacgg gctactcggg tttccgcgtt cgtgcgccgc tgaactcgcc cgattatctc
      421 gacgagttcc tcgtgttcca gggcgcgagc tactttcgcg ccgtcgcgaa ggggcagctc
      481 tacggaatat cggcgcgcgg gctggccgtc cgcacggcga ggccggaggg ggaggagttt
      541 ccgtacttca ccgatttctg gatcgagcgt ccgccggaag gcgcgaccga gatcgtgatc
      601 cacgcgctgc tgcagagccc gtcggtcgtc ggcgcctaca cgttcaaggc ccggcccggc
      661 gaggaaacga tcgtcgacgt cgatgcggcg ctgttcccgc gcgtcgacct gaccgcattc
      721 ggcatcgcgc cgctgatgtc gatgttcttg ttcaacgcgt cgaaccgctt ccgcttcgac
      781 gactaccgcg cggccgttca cgactccgat gggctgcaga tattgaacgg gcgcggtgaa
      841 cggctgtggc gcccgctcgc gaatccgcga atgacctcgc aaacctctgc cttcgtcgac
      901 gacagcccga aagggttcgg cctgctccag cgcaagcgca atttcgcgga ctatcaggac
      961 gccgaagcgc aatacgagcg gcggccgtcg ctctgggtcg agcccgccac cgagtggggg
     1021 cccggccacg tcgagctcgt cgagattccc tcgccgcgcg tcaacgacaa catcgtcgcg
     1081 tactggcagc cgcgcgagcc gatcttggcc ggacagagcc gcgaattctc gtatcggctg
     1141 cggttcaccg acgagccgct cgacgattct ctcgcgcgcg tcgtcgcgac gcgcgtcggg
     1201 caggcgccga acgccgaagg ccagcgcgcg ttcgtcatcg atttcaaagg cgccggggac
     1261 atccccgacg acctcgtccc cgaggtctgg agctcggcgg gcgaagtctt tgcgccgcgc
     1321 ggccaagtcg tggcgcaggc cggcacgtat cgcgtggcgt tcgagctgga tcccaagcgg
     1381 gaaaacctaa tcgagatggg cgtcgttctg cgctcgcagg gcaaaccttg gagcgagacg
     1441 tggttgtacc agtggagccg atgacgctgc gccattcgag ctcggtgccg ccgttcgccg
     1501 agctcgacat gcccgagcaa aacttgcggc gctacgttcg cgagcgccgg ctcgcacgtc
     1561 cgtcgttctt cacggtgctc gcgcgtctcc tcacgttcgg cggcgcattg gcgctcaccg
     1621 tctacgcgac gctcgagatg atcgccgtcg tgtcggtcgg catcgtgtcg gcgctgcagt
     1681 gggcgatggt cgcgctgttc gcgatcacgt tcgcttggat cgcgctgccg gccacggccg
     1741 ccgtctcggg cgtgctgttc ggctgggtgc gccgccgcgc gaaggcggat ctacgcgtcg
     1801 agcagcgcac ggcgctcgtg atgccggtct acaacgagaa cccggccggc acgttcgccg
     1861 cattgctcgc gatggccgag gcgctgaccg agcagaacgc cacgggcttc gagatcttcg
     1921 tgctttccga cacaacgcgc cccgagatct acgtgaaaga aacagccgcg tatcacgtgc
     1981 tgcgcgagaa gctcggcgag cgaatgcgcg tctggtatcg gcgccgcccg gacaacgtcg
     2041 gccgcaaggt cggcaacctt cacgatttcg tgacgcgctg gggcgggcgc tacgacttca
     2101 tgg
//
LOCUS       DASSNN010000766         3743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_873739, whole genome shotgun sequence.
ACCESSION   DASSNN010000766 DASSNN010000000
VERSION     DASSNN010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873739"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2257)
                     /locus_tag="VFL84_14385"
     CDS             complement(<1..2257)
                     /locus_tag="VFL84_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7609865.1"
                     /translation="MSSLSTISRAVRSALSAPYAITATVACGVCVVGAPVWAQQAAGA
                     DTGDTITVTGSRLRRDAFNSVSPVTLITREEVTAAGFTSATDALQGTAVTNGGAQINN
                     AFGGFVTDGGPGANTLSLRGLGAGRTLVLINGRRVAPSGTRGAVGSADLNVLPNAIID
                     HVEVLRDGASSIYGSDAIAGVVNIVTLEEPSGITFEAQSNNPLDGGGEQNRVSMVAGW
                     SGEKWSFGGSLEYYDRAELTQADRDWTACNVDGRRDPDTGASLDFTDPLTGQPKCYPI
                     TGTGSNGVTINTIGTQNVTAANAGSLGLTGTVVGAPGSTGNNTTFNRWRPNPTVTTGV
                     VGFEGVGGGGNNLNVRDTFEPRMRNKSLISPGENSTAYLQAGRGLDILGNAELYFDFL
                     AHQRESAQTGYRQLALDYRLGSPLIPSNLSFSDFGPDQGTSGGSRVGVRAFIGFGNDE
                     NKQSIEFYKPSAGLRGDIGENWRYDANVSFSTSDAKYKQDSWLTDKLIYAGDVVAAPS
                     GTDPALVRNGLTCRINLTNPAERCIPYPPLDNAVIAGIMPADFVDYTWRDAIGHTEYD
                     EDVYAVTFDGPLAALPAGRLQAAFGFEHRDAEIDDTPDANSIAGNLYNLTSATPTRGK
                     DSVDEVFTEIEVPLIANKKGAYELTANVSMRYTDYDSYGSDDTYKVGLMYSPVNWFSV
                     RVTQGTSFRAPALFEQFQGPTSGFLSQAGDPCNNYGVKVGPVVANCASEGLPPTFNAT
                     SSIRVFSEGGAA"
     gene            complement(2402..>3743)
                     /locus_tag="VFL84_14390"
     CDS             complement(2402..>3743)
                     /locus_tag="VFL84_14390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7609866.1"
                     /translation="RLPDIDGFEVCKALRRNPSLPSLAIVQMSATFEAAEYKVRALEG
                     GADTFLSDPVEPTVLVATVRAMLRLRHAEMQLREFDRRKDEFLATVAHELRNPLAPLR
                     HCVEWLEQSPDAVSALQACLPIMRRQSDHLIRLVDDLADMSRITQNKLTLRLSSVDLL
                     QTLEAAIDEHRAELEAKEQELDLDLPERPLILAADAVRLSQVFGNLLANGIRYTPRGG
                     RISVAVRETGAHATVTFADTGEGIDPKDIDRIFDLFVQVAPHRTGLGIGLALVHRIVD
                     MHGGSISASSPGRGAGSTFTVRLPLGAPEVVDDERRDAVQFRESLAPRATVPTPRRVL
                     VVDDNVDVADSLAQLLKAMDHDVRVAYRGPDGVAAYSEFAPDVVLMDINMPGMDGLEA
                     IAKIRAVTGGDRTLICTLSGHGKAHAQRAFEAGADGHLVKPIGRAELSAVLERA"
BASE COUNT          594 a         1229 c         1275 g          645 t
ORIGIN      
        1 ccgcggctcc gccttcgctg aacactcgaa tgctgctcgt cgcgttgaag gtcggcggca
       61 gcccttccga ggcgcagttg gcgacgaccg gaccaacctt gacgccatag ttgttgcagg
      121 ggtcgccggc ttggctcaag aagccgctcg tcgggccttg gaactgctcg aagagcgcgg
      181 gagcgcggaa cgacgtgcct tgcgtgaccc gtacggagaa ccagttgacc gggctgtaca
      241 tcaagccgac cttgtatgtg tcgtcggagc cgtacgagtc gtagtccgta tagcgcatcg
      301 acacgttcgc ggtcagctcg tacgcgccct tcttgttggc gatcagcggc acctcgatct
      361 ccgtgaacac ctcgtcgacg ctgtccttgc cgcgcgtcgg cgtggcgctc gtcaggttgt
      421 agaggttgcc cgcgatgctg ttcgcatcgg gcgtgtcgtc gatctcggcg tcccgatgct
      481 cgaagccgaa cgcggcctgc agacggccgg ccggcagcgc cgcgagcgga ccgtcgaacg
      541 tcacggcgta cacgtcttcg tcgtactccg tgtggccgat ggcgtcgcgc caggtgtagt
      601 cgacgaaatc ggccggcatg atgccggcga tgaccgcgtt atcgagcggc ggatacggaa
      661 tgcaccgctc cgccggattc gtcaggttga tcctgcaagt caggccgttg cgaacgagcg
      721 cgggatcggt gcccgacggc gcggcgacga cgtcgccggc atagatcagc ttgtcggtca
      781 accacgagtc ttgcttgtac ttggcgtcgg acgtcgaaaa gctgacgttg gcgtcgtagc
      841 gccagttctc gccgatgtcg ccgcgtagcc cggcggacgg cttatagaac tcgatcgact
      901 gcttgttctc gtcgttaccg aagccgatga aggcacgcac gcccactcgc gagccgccgc
      961 tcgttccctg atccggaccg aagtcgctga acgagagatt tgacggaatc agtgggctgc
     1021 ccaagcgata gtcgagcgcc agctggcgat aacccgtttg cgcagactcg cgctggtgcg
     1081 ccaagaagtc gaagtagagc tccgcattgc cgaggatgtc gaggccacgc cccgcctgca
     1141 aataggccgt cgagttttcg cccggcgaga tcaggctctt gttcctcatg cgaggctcga
     1201 acgtatcgcg cacgttcaag ttgttcccgc cgccaccgac gccttcgaag ccgacgacgc
     1261 ccgtcgtgac ggtggggttc gggcgccaac gattgaacgt cgtgttgttg cccgtggagc
     1321 ccggtgcgcc gacgaccgtg cccgtcaagc ccaggcttcc ggcgttggcc gccgtgacgt
     1381 tctgcgtgcc gatcgtgttg atcgtgacgc cgttcgagcc cgtgcccgtg atcggatagc
     1441 acttgggctg ccccgtgagc gggtcggtga agtcgagcga cgcgcccgtg tccggatcgc
     1501 gccgaccgtc gacgttgcac gccgtccagt cgcggtctgc ctgcgtgagc tcggcgcggt
     1561 cgtagtattc gagcgacccg ccgaacgacc acttctcgcc ggaccagcca gcgaccatgg
     1621 agacgcggtt ctgctcgccg ccgccgtcga gcggattgtt cgactgagcc tcgaacgtga
     1681 tgcccgacgg ctcttcgagc gtgacgatgt tgacgacacc ggcgatggcg tccgatccat
     1741 agatcgagga cgcgccgtcg cgcagcacct cgacgtggtc gatgatcgcg ttcggcagca
     1801 cgttcaggtc ggccgagccg accgcgccgc gcgtgccgga cggcgcgacg cgccggccgt
     1861 tgatcagcac caacgtgcgt ccggcgccga ggccgcgcag cgacaacgtg ttcgcgccgg
     1921 ggccgccgtc ggtgacgaaa ccgccgaacg cattgttgat ctgcgctccg ccgttcgtga
     1981 cggcggtgcc ctgcagcgcg tcggtcgcgg acgtgaaacc ggcggccgtg acttcctcac
     2041 gcgtaatcag cgtgacgggc gagaccgagt tgaacgcgtc gcggcgcaac cgcgagccgg
     2101 tcacggtgat ggtgtcgccc gtgtcggctc ccgccgcctg ttgcgcccaa acgggcgcgc
     2161 cgacgacaca aacgccacaa gcgaccgtgg ccgtgatcgc atacggcgcg gacagcgcgg
     2221 aacgcaccgc acgcgagatc gtgcttagac tcgacatttc gttgatcccc cctaatttag
     2281 ttggttgcag tgggccccct gaggcccgcc gcgttggcgg caagtgtaac gggccccatg
     2341 acaaatttgt aatgtgggtg ggcccgttgt ggcgggcgtg caacagtcgg agatcggcgg
     2401 ctcaggcccg ctcgagaacg gcgctgagct cggcgcggcc gatgggcttc acgaggtgac
     2461 cgtcggcgcc cgcctcgaat gcgcgctggg catgcgcctt gccgtggccg ctcagggtac
     2521 agatcaacgt cctgtcgccg cccgtcaccg cgcggatctt ggcaatggct tcgaggccgt
     2581 ccatgcccgg catgttgatg tccatgagga cgacgtcggg tgcgaactcc gaatacgccg
     2641 ctacgccgtc gggtccgcgg taggcgactc gcacgtcgtg gtccatcgcc ttgagcagct
     2701 gcgcgagcga atccgcgacg tcgacgttgt cgtcgacgac gagaacgcgc cgcggcgtcg
     2761 gcaccgtcgc cctcggcgcc aacgactcgc ggaactgcac ggcgtcgcgg cgctcgtcgt
     2821 ccacgacttc gggcgccccg agcggcaggc gcaccgtgaa cgtgctgccg gcgccgcggc
     2881 cggggctcga agccgaaatc gagccgccgt gcatgtccac gatccggtgc acgagcgcga
     2941 ggccgatgcc gagcccggtc ctgtgaggcg cgacctgcac gaacaagtcg aagatgcggt
     3001 cgatgtcctt cgggtcgatg ccttcgccgg tatcggcgaa cgtcacggtc gcgtgcgcgc
     3061 cggtctcgcg aaccgcgacg gagatgcgcc cgccgcgcgg cgtgtagcgg atgccgttcg
     3121 cgagcaagtt gccgaagact tgcgagaggc gaaccgcgtc ggccgcgaga atcagcgggc
     3181 gctcgggcag atcgaggtcg agctcttgct ccttcgcttc tagctcggcg cgatgctcgt
     3241 cgatcgcagc ctccagcgtc tgcaggaggt cgacggagga caaccgcaac gtcagcttgt
     3301 tctgcgtgat ccggctcatg tcggcgagat cgtcgacgag ccggatcaag tggtcggact
     3361 gccgccgcat gatcggcaag caggcctgca acgcggagac cgcgtccggc gattgctcga
     3421 gccactcgac gcaatgacgc agcggcgcga gcggattgcg gagctcgtgc gccacggtcg
     3481 ccaagaattc gtccttgcgc cgatcgaact cgcgcagctg catctcggcg tgacgaaggc
     3541 gcagcatcgc ccgcacggtc gcgacgagca cggtgggctc gacgggatct gacagaaacg
     3601 tgtcggcgcc gccttcgagc gcgcgcactt tgtactccgc cgcttcgaac gtggccgaca
     3661 tttgcacgat tgcgagcgac ggcaggctcg gattccgccg cagcgctttg cacacctcga
     3721 atccgtcgat gtcgggcagg cgg
//
LOCUS       DASSNN010000767         4581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1882245, whole genome shotgun sequence.
ACCESSION   DASSNN010000767 DASSNN010000000
VERSION     DASSNN010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1882245"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VFL84_14395"
     CDS             complement(<1..696)
                     /locus_tag="VFL84_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609867.1"
                     /translation="MSARILVFGLMAAVALAACSKSTPSGGADTQAADASAGKTLRTA
                     WGDPDLQGMWPITNLVSTPFQRPVDQQGNPIYGDRELLTDEEYAAAQQRLAARDQRYQ
                     DEIKSNKMGMGHWAEASHNNDAARLTSLLVEPKNGRFPELTPRGKELAAKMKSSWSGE
                     VFDKPTDFDTWDRCITRGLPPSMFPFMYNNGIEIIQAPGYVIVRLEMIHEARVIPVDG
                     RRPLDPAIKQWMGE"
     gene            911..2926
                     /locus_tag="VFL84_14400"
     CDS             911..2926
                     /locus_tag="VFL84_14400"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7609868.1"
                     /translation="MHAAHRCIGLGFALALAACGEAPEQANTAAPAAGSAPPAGVAPG
                     DWPLINRDLSANRYSPLAEITTANVANLAPSWTYQLGGNSSAVPVVVAGVMYLPSRDR
                     VVALDGDSGALVWEYVLPAPPAPPASAAPPEGAGAGGGGGPGGPGGGPRGPSASTRGV
                     SYWAGDGTLPPRILFMSGANLIALDAATGTPAAGFGTNGAVNVGVPYGGTPTVFDSVA
                     IIGAASGEVPQGPPGNPRAFDVRTGAKLWEFQTVPRPGEPFNDTWGNGWEGRGGTNMW
                     GFAAPIDAALGLAYLPIAGPAANYYGGDRPGNNVFGNSIVAVDARSGAYKWHFQTVHH
                     DLWDTDMPSAGALLEVERNGTRVPVIAHVGKSAYFYVLDRTTGQPFHEVRETPVPKGD
                     VPTEWYSPTQPIPVRPPPLARVSFSEADLVRPEDTTPEHAAACRAFMEKSGGFYNAGP
                     FTPFMYKAPEAPPKSTIQFPGGTGGVNWGGVAIDPTTGFVFAASHDGALVGWVQDKDP
                     NVTYSFEARGSKQPYDRASINGVGPFFTFSAPLSGQYNERGQGVGPSAPCQRPPWGKL
                     VAVNANTGEIAWESVLGLNENLPEGKQLVGSAGSAGPSVTAGGLVFVGNTSDRRFRAF
                     DARTGAELWSAMLASNAGANPMSYLGSSGKQRVAIVAGDTVNVFALP"
     gene            3008..3826
                     /locus_tag="VFL84_14405"
     CDS             3008..3826
                     /locus_tag="VFL84_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609869.1"
                     /translation="MQLNKKIALAILVASAALGAAPSIAQQSGAAARPARIAGHPNIN
                     GIWQAMNSANWNLEAHSATKLDEFWALGSLAAIPAGQSVVDGDGKIPYKPEALAQRDE
                     NRKGWPKSDPETSCYLPGIPRATYMPFPFQIVQGGGDILFVYTYASANRLVNMGEPIE
                     PPVDTWMGQSNGRWENETLVIETTGFNDRTHFDRSGNYHTSSLKVTERFTPVSENHLQ
                     YSATIEDPNVFTRPWTINMPLYRHIEPNAQILEFKCVPFVEELLYKDLELPQQR"
     gene            3944..4363
                     /locus_tag="VFL84_14410"
     CDS             3944..4363
                     /locus_tag="VFL84_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609870.1"
                     /translation="MRTNLISLVAGVGLSLTAAVPALAHHSFAAEFDANKPVTIEGTV
                     KEFRWVNPHSWLHINVTKPDGTVEEWAVEGGAPSALLRRGWTRDTLPPGTKVKVEGFM
                     SKDGALRANARDITFPDGKKMFVGSSGTGAPDERPEQ"
BASE COUNT          808 a         1514 c         1528 g          731 t
ORIGIN      
        1 ctcgcccatc cactgcttga ttgcgggatc gagcggacgg cggccgtcga cggggatcac
       61 gcgcgcctcg tggatcatct cgagccgcac gatcacgtag cctggggcct gaatgatctc
      121 gatgccgttg ttgtacatga acgggaacat cgacggcggc aaaccgcgcg tgatgcaccg
      181 atcccacgtg tcgaagtcgg tcggcttgtc gaagacctcg ccgctccagc tgctcttcat
      241 cttggcggcg agctccttgc cgcgcggcgt cagctcgggg aaacgcccgt tcttcggctc
      301 gacgagcaat gaagtcaagc gagccgcatc gttgttgtgg ctcgcctcgg cccagtggcc
      361 catgcccatc ttgttgctct tgatctcgtc ctgatagcgc tgatcgcgag ccgcgagccg
      421 ttgctgagct gccgcgtact cttcgtccgt gagcagctcg cggtcgccgt agatcggatt
      481 gccctgctga tcgaccggcc gctggaacgg cgtgctgacc aaattcgtga tcggccacat
      541 gccttgcagg tcggggtcgc cccacgccgt gcggagcgtc ttgcccgctg acgcgtcggc
      601 cgcctgcgta tcggcgccac ctgacggtgt cgacttcgag cacgcggcga gcgcgactgc
      661 cgccatgaga ccgaagacga ggatccgcgc gctcatcaca acctccacag gcccactggc
      721 caaaaaacca gcctggccgc cgcatcgatg ggatgcaacc aaagtttctt taggacgccc
      781 ttagctcgcg cgccgcgcga atccgctccc ccagggcgtc gccgcaaaag tatacggcgg
      841 gcccgccccg cgcgggaacg gtgtatctta cggaccctaa gcgaagctca ggtgttttgg
      901 gggaacgttc atgcacgctg ctcaccgttg cattggtttg ggctttgctc tcgcgctggc
      961 cgcgtgcggc gaagcgcccg agcaagccaa tactgcggca ccggccgcgg ggtccgcgcc
     1021 gccggccggc gtcgcgcccg gcgactggcc gctgatcaac cgcgatctca gcgcgaaccg
     1081 ctactcgcct ttggccgaga tcacgaccgc gaacgtcgcg aatctcgcgc cgagctggac
     1141 gtatcagctc ggcggcaact cctcggcggt cccggtcgtc gtcgcaggcg tcatgtactt
     1201 gccgagccgc gaccgcgtcg tcgcgctcga cggcgacagc ggcgcgctcg tctgggaata
     1261 cgtgctaccc gccccgcctg cgccgcccgc gagcgcggcg ccgcccgaag gcgccggcgc
     1321 cggtggcggc ggcgggcccg gcggccccgg cggcggtccg cgcggtccca gcgcttcgac
     1381 gcgcggcgtg agctattggg cgggcgacgg cactctgccg cctcgcatcc tgttcatgag
     1441 cggagcgaat ttgatcgcgc tcgacgcggc gaccggaacg cccgccgcgg gcttcggaac
     1501 aaacggcgcg gtcaacgtcg gcgtgccgta cggcggaacg ccgacggtgt tcgatagcgt
     1561 cgcgatcatc ggcgcggcaa gcggtgaagt gccgcaagga ccgcccggca atccgcgtgc
     1621 cttcgacgtg cgcaccggcg cgaagctctg ggagttccaa acggtgccgc gcccgggcga
     1681 gccgttcaac gacacttggg ggaacggttg ggaggggcgc ggcggcacca acatgtgggg
     1741 cttcgccgcg ccgatcgacg cggcgcttgg gctagcgtat ttgccgatcg cagggcccgc
     1801 cgcgaactac tacggcggcg accggcccgg caacaacgtc ttcggcaact cgatcgtcgc
     1861 cgtcgacgcg cgcagcggtg cgtacaagtg gcatttccaa accgtgcacc acgatctttg
     1921 ggacacggat atgccgtcgg cgggtgccct gctcgaggtg gagcgcaatg gcacgcgcgt
     1981 gcccgtcatc gcgcacgtcg gcaagtcggc gtacttctac gtgctcgacc gcacgaccgg
     2041 acagccgttt cacgaagtgc gcgagacgcc ggtgccgaaa ggcgatgtgc cgaccgagtg
     2101 gtattcgccg acgcagccga tcccggtgcg cccgccgccg ctcgcgcgcg tcagcttcag
     2161 cgaggccgat ctcgtccgcc ccgaagacac gacgcccgag cacgcagccg cgtgccgcgc
     2221 cttcatggag aagagcggcg gcttctacaa cgccggcccg ttcacgccgt tcatgtacaa
     2281 ggcgcctgaa gcgccgccga agtcgacgat ccaattcccg ggcggcacgg gcggcgtgaa
     2341 ctggggcggc gtcgcgatcg atccgacgac gggcttcgtg ttcgcggcat cgcacgacgg
     2401 cgcgctggtc ggctgggttc aggacaagga tccgaacgtc acgtacagct tcgaagcccg
     2461 cggctcgaag cagccgtacg accgcgccag catcaacggt gtcggtccgt tcttcacgtt
     2521 cagcgcgccc ttgagcggcc agtacaacga gcgcggtcag ggcgtcggcc cgagcgcgcc
     2581 gtgccagcgt ccgccgtggg gcaagctcgt cgccgtcaac gcgaacacgg gcgagatcgc
     2641 ctgggagagc gtgctcggct tgaacgagaa cctgcccgag ggcaagcagc tcgtcggcag
     2701 cgcgggcagc gctggtccgt ccgtgacggc cggcggcctc gtgttcgtcg gcaacacgag
     2761 cgatcgccgt ttccgcgcgt tcgacgcacg cacgggagcg gagttgtgga gcgcgatgct
     2821 cgcgagcaat gccggcgcga acccgatgag ctacttagga agcagcggca agcagcgcgt
     2881 cgcgatcgtc gccggcgata ccgtcaacgt gttcgcgctg ccataagggc gaaaatgacg
     2941 gcggagtgac gtttcactta tgattcgcgg cttgtcgatt cgcagaagta caactagggg
     3001 attctgcatg cagctcaaca agaagatcgc cttggcgatt ctcgtcgcat cggccgctct
     3061 cggcgccgcg ccaagcattg cgcagcagtc gggcgcggcg gcacgtcccg cgcgcatcgc
     3121 cgggcatccg aacatcaacg gcatctggca ggcgatgaac agcgcgaact ggaatctcga
     3181 ggcgcactcg gcgacgaagc tcgacgagtt ctgggcgctc ggctcactcg ccgcgattcc
     3241 ggccgggcaa agcgtcgtcg acggcgacgg caagatcccc tacaagccgg aggcgctcgc
     3301 gcaacgcgac gagaaccgca agggctggcc gaagtcggat ccggagacga gctgttatct
     3361 gcccggcatt ccgcgcgcga cgtacatgcc gtttccgttc cagatcgtcc aaggcggcgg
     3421 cgacatcttg ttcgtgtata cgtacgcgag cgcgaaccgg ctcgtgaaca tgggcgagcc
     3481 gatcgagccg cccgtcgaca cgtggatggg gcagtcgaac ggccgctggg aaaacgagac
     3541 gctcgtcatc gaaacgacgg gcttcaacga ccgcacgcac ttcgaccgct ccggcaacta
     3601 tcacacgagc tcgctgaagg taacggaacg gttcacgccc gtcagcgaga atcacttgca
     3661 atattcggcg acgatcgagg acccgaacgt cttcacacgg ccgtggacga tcaacatgcc
     3721 gctgtaccgg cacatcgagc cgaacgcgca gatcctcgag ttcaagtgcg tgccgttcgt
     3781 cgaggagctg ctctacaagg atttggagct gccgcagcag cgatagccgg ggccaaatca
     3841 caggatttgc caaggtggga ttgcgtcgac cggcggccgg cccgagatcg agataaacta
     3901 gcgaccaaga tcggttctac ctggaacctt gaggagacga tagatgcgta cgaacctaat
     3961 cagcctggtc gcgggcgtcg ggctatcgct gacggcagcg gtaccggcac tcgcgcatca
     4021 ctcgttcgcc gcggaattcg atgcgaacaa gcccgtcacg atcgagggca ccgtcaagga
     4081 gttccgctgg gtcaacccgc attcgtggct ccacatcaac gtgacgaagc cggacggcac
     4141 cgtcgaagag tgggccgtcg aaggcggcgc gccgagcgcg ctgctacgcc gcggctggac
     4201 gcgtgatacg ttgccgccgg gcacgaaggt caaggtcgaa ggcttcatgt cgaaggacgg
     4261 cgcgctgcgc gcgaacgccc gcgacatcac cttccccgac ggcaagaaga tgttcgtcgg
     4321 ctcgtcgggc accggcgcgc cggacgagcg gccggagcaa taacactccg ccgacacgct
     4381 caacgacgaa tcaaccgggg gcctcgcgcc cccggttgcg tttaaaaagg ggacagattt
     4441 attttttgga agaggcttgg aagaaaatag atccgttcct ttttcggaaa ataaatctgt
     4501 ccccttttcg gtcaggattt ctgggcggtg tcgagggacg cgcgaagctc agccggcagt
     4561 cccaaccgcg cggcgagggc g
//
LOCUS       DASSNN010000768         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_997075, whole genome shotgun sequence.
ACCESSION   DASSNN010000768 DASSNN010000000
VERSION     DASSNN010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997075"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /gene="hrcA"
                     /locus_tag="VFL84_14415"
     CDS             complement(<1..869)
                     /gene="hrcA"
                     /locus_tag="VFL84_14415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET7609871.1"
                     /translation="MASDRPRDILDERAQILLRLLVDKYIREGQPVGSRTLSREAGLD
                     LSPATIRNIVSDLEELGFVKSPHTSAGRVPTIKGYRFFVDTLIKLTPPRGEELKRFSE
                     SLSEASGDPQALALSVSNLLSAVTSLAGIVTVPRRAQTAVRQIEFLPLSGHRVLAILV
                     INDREVQNKILNVSRDFGADELKRASNFLNEHCGGRSLPEIRELLLAELKRTRASMNA
                     LMVEAIEMAQKVFPESEPAPEFVVAGETNLMGIDQVADVETLKGLFEAFGRQRDILHL
                     LDQSMKADGVQIF"
     gene            957..1853
                     /locus_tag="VFL84_14420"
     CDS             957..1853
                     /locus_tag="VFL84_14420"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002306.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HET7609872.1"
                     /translation="MPRQFKTVALWGRLGERSVTEPARQVLSHLRKRGITVLASITSD
                     PTRELADATHVDEREAAKRADLVIAIGGDGTLLHAARNVAARNVPLVGINRGRLGFLT
                     DVTPEHLRDALDAILKGNYLAERRLTLAARLGKRAKVSLFALNDIVLQKGDTGRLLDF
                     TTEVDDVYVNTHRGDGLIVATPTGSTAYALSCGGPIIQPNVDALVMVPICPHTLSDRP
                     LVLPSSSEIRVTLDNPGGSEAHVVCDGESLARMSAGDALTISLSKQPVTLLHPREYNY
                     YELLRSKLNWGRASRGQSNGRR"
     gene            1864..>3011
                     /locus_tag="VFL84_14425"
     CDS             1864..>3011
                     /locus_tag="VFL84_14425"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7609873.1"
                     /translation="MLSLIRVKNYAVIDEIEVEFGGGLNVMTGETGAGKSILVDALGL
                     ALGDRADASAVRRDAERAEISVSFDVPGRHEALRWLAERGLDDDTACTLRRLVGSDGR
                     SRAFINGQPVNLQDLKSLGELLVDIQGQHAHQSLLDAGNQRALLDAHDEVAPVAKTVA
                     ENFAAWHTLVEQLENRRSSSAQRESEIELVRFQIAELEALAIAEREPERLKAERDRLA
                     NTDRLLSGATAALDALAENESASAYAAVVLARREIEKLAEVDAALRPPATALASIEIE
                     LREVETALQHYRDRIEADPARLAWLDDRLARVRTLARRHGAGEDDLPRVLASLRGRLA
                     ALDGGGESLEALEKRTAEARTQFLATARRLSGLRAKHAAALGRAVTGLL"
BASE COUNT          522 a         1018 c          993 g          478 t
ORIGIN      
        1 atgaaaatct ggacgccgtc ggccttcatg ctctgatcga gcaaatgcaa aatgtcgcgc
       61 tgacggccga acgcttcgaa taggcctttc agcgtctcga cgtccgcgac ctggtcgatc
      121 cccatcaggt tcgtttcgcc cgctacgacg aactcgggcg ccggctcgga ctccggaaac
      181 accttttgcg ccatttcgat cgcctcgacc atcagcgcat tcatcgaggc gcgcgtgcgc
      241 ttgagctccg cgagcagcag ctcgcggatc tccggcaggc tgcgaccgcc gcaatgctcg
      301 ttcaagaagt tgctcgcgcg cttgagctcg tcggcgccga aatctcgcga aacgttgagg
      361 attttgttct ggacctcgcg gtcgttgatg acgaggatgg caagcactcg atgacccgac
      421 agcggcaaga actcgatctg tcgcaccgca gtttgcgcgc ggcgcggcac cgtgacgatt
      481 ccggcgaggc tcgtcacggc ggagagcagg ttcgaaacgg acagcgccag cgcctgcggg
      541 tcgcccgacg cttcgctcaa gctctcgctg aagcgcttga gctcttcgcc tctcggcggc
      601 gtgagcttga tcaaagtgtc gacgaaaaag cggtagccct tgatcgtcgg aactcggccg
      661 gccgacgtgt gcggcgattt cacgaacccg agctcttcga gatcggagac gatgttgcga
      721 atcgttgcag gagagagatc caacccggcc tcgcgcgaga gcgttcgaga gccgaccggc
      781 tgtccctcgc gaatgtattt atcgacgaga aggcgcagca gaatctgtgc gcgttcgtcc
      841 aggatgtccc taggtctgtc gctcgccata ggcccaggct gtgataacgt agagcccgct
      901 tttttagcga ccagcacgct aacaacgggt tcccgccact gtcaagaaag caaaccatgc
      961 cgcgtcagtt caagaccgtc gcgctgtggg ggcgcctcgg cgaacgcagc gtgacggagc
     1021 ccgcgcggca agttctatct cacctgcgca aacgcggcat cacggtcctc gcctccatta
     1081 cgtccgatcc gacgcgcgag ctcgccgatg ccacgcatgt cgacgagcgc gaagcggcaa
     1141 aacgcgccga cctcgtgatc gcgatcggcg gcgacggcac cttactgcac gccgcgcgca
     1201 acgtcgccgc gcgcaacgtg ccgctggtcg gcatcaatcg cggccgtttg ggatttctca
     1261 cggacgtgac gcccgagcac ttgcgcgacg cgctcgacgc gatcttgaag ggcaactatc
     1321 tcgccgagcg ccggctcacg ctcgccgctc ggctcggcaa gcgcgcgaag gtctcgctgt
     1381 tcgcgctcaa cgacatcgtg ctgcagaagg gcgacacggg ccggcttctc gacttcacga
     1441 ccgaggtcga cgacgtctac gtcaacactc atcgcggcga cggactgatc gtcgcgacgc
     1501 cgacgggctc cacggcttac gccctatcct gcggcggtcc gatcatccag cccaacgtgg
     1561 atgcgctcgt gatggtgccg atctgcccgc acacgctgag cgacaggccg ctcgtgctgc
     1621 cgagctcgag cgagatccgc gtgacgctcg acaatcccgg cggcagcgaa gcgcacgtcg
     1681 tttgcgacgg cgagtcgctg gcgcgaatgt cggcgggaga cgcgctgacg atctcgttgt
     1741 cgaagcagcc cgtcacgctg ctgcatccgc gcgaatacaa ctactacgag ctgttgcgct
     1801 cgaagttgaa ctgggggcga gcgagccgcg gtcagtcgaa cggccgccgc tagcgggcgc
     1861 gtcatgctga gcttgatccg cgtcaagaac tacgccgtca tcgacgagat cgaggtcgag
     1921 ttcggcggcg gcctgaacgt catgaccggc gaaacgggcg ccggaaaatc gatcctggtc
     1981 gacgcgctgg ggctcgcgct cggcgatcgc gccgacgcgt cggccgtgcg ccgcgacgcg
     2041 gagcgcgccg agatcagcgt ctcgttcgac gtgccgggcc gccacgaggc gctgcgctgg
     2101 ctcgccgagc gcggcctcga cgacgacact gcgtgcacgc tgcgcaggct ggtcggctcg
     2161 gacggacgct cgcgcgcgtt catcaatggc cagcccgtga acctccagga cttgaagtcc
     2221 ctcggggagc tgctcgtcga catccaaggg cagcacgcgc accagtcgtt gctcgacgcc
     2281 ggcaaccagc gagcgttgct cgacgcgcac gacgaggtcg cgcccgtcgc caagacggtc
     2341 gcggaaaatt tcgccgcttg gcacacgctc gtcgagcagc tcgagaaccg ccgctcgtcg
     2401 agcgcgcaac gggaatccga aatcgagctc gtgcgctttc agatcgccga gctcgaagcg
     2461 ctggcgatag ccgaacgcga gcccgagcgt ctcaaagccg aacgcgaccg gctcgccaac
     2521 acggaccgtt tgctgagcgg cgccaccgcc gcgctcgacg cgctggccga gaacgaatcg
     2581 gcttcggcct acgccgccgt cgtgctcgcg cgccgcgaga tcgagaagct tgccgaagtc
     2641 gacgcggcct tgcggccgcc ggcgacggcg ctcgcgagca tcgagatcga gctgcgcgag
     2701 gtcgagacgg cgctgcagca ctaccgcgat cgcatcgaag cggatccggc ccgcttggcg
     2761 tggctcgacg accggctcgc gcgcgtgcgc acgctcgccc gcaggcatgg cgccggcgag
     2821 gacgacctgc cgcgcgtgct ggcgtcgctg cgcgggcgcc tcgcagcgct cgacggcggc
     2881 ggcgagtcgc tcgaggcgct cgagaagcga acggcagagg cgcggacgca attcctcgcg
     2941 accgcgcgcc gcttgagcgg cttgcgcgcg aagcacgcgg ctgcactagg ccgcgcggtc
     3001 acgggattgc t
//
LOCUS       DASSNN010000769        10179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1883482, whole genome shotgun sequence.
ACCESSION   DASSNN010000769 DASSNN010000000
VERSION     DASSNN010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10179
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883482"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VFL84_14430"
     CDS             complement(<1..399)
                     /locus_tag="VFL84_14430"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7609874.1"
                     /translation="MRRSIVTVLVACVPLAAAGGPLIDAVKRQDAAAASALLDRGAAV
                     DEAEADGTTALHWAAERDDLETVELLLDAGAAVTAANRFGVTPLELAANNGNGEIVER
                     LLASGADPNARSREGQTPLMSAALNGQPAAV"
     gene            532..2877
                     /locus_tag="VFL84_14435"
     CDS             532..2877
                     /locus_tag="VFL84_14435"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET7609875.1"
                     /translation="MKRYVVGISVFVVTAAAAAWIARHSDEAATRGLLGRYCTDCHNK
                     TDFTAELVIEPTDVASIAADPEHWEKIVRKLRAEEMPPDGPRPQHAQYQRAAAFLASR
                     LDAAAAAEPQPGAVPAFRRLTRTEYKNAIRDLLALDGLPAELDFELLLPADNAASGFD
                     NIADLLFVSPVVMERYIAAAQKIARLAVGDLRAPVMVNIHRLSEQLPQDERIDGLSFG
                     TRGGLRVETYLPLDAEYVFEVETAAPVPEAHELEVSVDGVRVANATLLTPERRSGAPR
                     RSNEQATFRVPIAAGPHLIGITFVEHSEALDESIARGRMRGRGTLPAIAMATIRGPYA
                     ATGPGDTPSRSRIFVCRPNGAADEPACAQQILRTLAHKAYRRPATDADVADLMPFYEA
                     GRAEGNFDTGIQLALERLLVSPQFLYRIEREPAEPGAHLVSDIELASRLSFFLWSSIP
                     DDELLAAAESGSLRRPGVLRAQVQRMLADARSEAMVTNFAAQWLFLRDVAVKDPDIFL
                     FPDHDPSLRAALERETELFVGSVFRSGGSVLDLLTAKHTFLNERLAKHYGVPHIQGSY
                     FRRVELGGDSRRAGLLGQGSILTVTSYAARTSPVLRGKYVLDNLLASPPPPPPPDVPS
                     LATEDAKDGAPLTLREAMIRHRANPVCAACHAKMDPIGFALEHFDAVGRWRDEDAGMP
                     IDSRSELADGRVIDGPEGVQAFLTSRPELFVSALTEKLLMYALGRNVQYYDAPAVRAV
                     VREAAARNYEFSAIVEGIVASVPFQMRATARDGVAAKENEQ"
     gene            2874..4235
                     /locus_tag="VFL84_14440"
     CDS             2874..4235
                     /locus_tag="VFL84_14440"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET7609876.1"
                     /translation="MKLETGKFVSRKALPRRTFLRGIGATLALPMLDAMVPAFAADRA
                     PWTPRLGFVYVGNGIVHKTFKLTGLGRDFEFSPVLKPLAPLRDYLTVVSGLDHAQANN
                     FGDGTGDHPRSSAAWLTGVHAYDRTQPGVEVKLTTTADQLAADVLGAHTPVRSLELAV
                     DTATQSACDSGDCFYVNTVSWRNETTPNLTENHPRVVFERLFGDGGNAADRSARTRKT
                     ASILDSVREEANRLAASVGHSDRSKLAEYLDAVREIEQRIGAAESETHGEVQLPERPL
                     GIPESFDDYAKLMLDLQLLAYQSDTTRVFSMILAREVSSRPYPHIGVPDQHHAASHHR
                     NDPGLIEKKTKIDAYHASLLGYLAEKMRTTSDGGGTLLDQSLLVYGGGMGDGNLHQHV
                     DLPCVLLGKLGGTVKAGQHVAYPSGTPMTNLLLTVLDKVGVTVAAIGDSTGRLSPDYL
                     TAV"
     gene            complement(4232..4498)
                     /locus_tag="VFL84_14445"
     CDS             complement(4232..4498)
                     /locus_tag="VFL84_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HET7609877.1"
                     /translation="MATGWAREGAVQDQIDDTVKDGVARAKSLIPQGPSATHCHECGE
                     PIPEARRAALPGVRLCIVCQQKADADVRVFSGYNRRGSKDSQLR"
     gene            4628..5002
                     /locus_tag="VFL84_14450"
     CDS             4628..5002
                     /locus_tag="VFL84_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609878.1"
                     /translation="MANAPRKRTAEQKPDALDEAFDKLEQAVPDSVTAAIRWMRSPQA
                     RIVRLPLGILCILGSFLWFLPVLGLWFLPLGLLLIAQDVPFMRRPVGRMTRWLLDRWE
                     HLRQWWSRKKEARAKRLRSESR"
     gene            5027..6142
                     /locus_tag="VFL84_14455"
     CDS             5027..6142
                     /locus_tag="VFL84_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET7609879.1"
                     /translation="MLPRMRVRSSTRQQKKKYRILLMVHETFVPPVETGIGAARLDEI
                     RTEVDVLRTLRRLGHEVIVVGIGGDLAELRETIDKWRPHVVFNLLDEFAGIISYDTHV
                     VAYLELLEQRYTGCNPRGMMLSRDKVLTKRVLATHGITTPAFKLFPFGRRFHAPARVR
                     FPLFVKSATEDASLGIAQASLCEDMRSLRERVELIHDQVQSDALVEEYIDGRELYVGV
                     LGNAKLVTLPPWEMDFGALPRDQARIATRRAKWNRRYQAKHGIDTGKARLRDDQHAHL
                     SRLSKRIYRALYMTGFARMDFRLCSDGRLFLLEANCNPNLSKGEDFADSAKAAGIGYT
                     ALVDRILQLGLASMPEWRQHAPRARTVRQNVKSTPIT"
     gene            complement(6112..9132)
                     /locus_tag="VFL84_14460"
     CDS             complement(6112..9132)
                     /locus_tag="VFL84_14460"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7609880.1"
                     /translation="MTRIESFSRALARTKLSLAVAGALCAGFAAAQEATPGPSGIDEV
                     TVTGTRITRDGMSSPTPITAISAEDMQMMAPGQFIDSLDYLPPFFMNDAPDTAASKSQ
                     SAGAANVNLRGLGANRTLVLLDGRRMVPSNRLGAVDINLFPEAMIERVEVVTGGASAV
                     YGTDAVAGVVNFILKDDFEGFDIHTQVGATERGDGENSEVSVAYGTSLGERGRLSVSG
                     EWFSADRIETLEGRDWFRHWGLIQNPVAGGPRDLVRPDVVSYTYTNGGLINSTLPGAG
                     PGADRIPTAIHRYEFLPDGSAQPFVAGNLIGANPATGPRVSTPGGSYGTHQITNGGSG
                     WDAVTDRGGSLVPETERGSLFAHYNFDLTDNASLYAQFLAGENKVNSVGTLPLGIAGW
                     AGTIYSGNPFLPANIQQLMTANNIQSFLLERYHTTADLAHDRFVTDNDTRSLTFGGES
                     QLQGGMFEGWTLGGYAQLGRNDNRITQVDFIRSDRLPEAMDVVADPLTGQPACRASFF
                     DPVRYGDCVPINLLGVGRASQQAIDYVLTGDQYILAETEQNVAEFSMEGEVGDGWGAG
                     AISLAFGGGWREESIGQTLGPADIIAGSLPAADSPTTRPDCVSNTVDLLNPPAGCTGI
                     RGVPAQFAATPNEIFIFTNVQPIAGSYSVSEVFAETLLPLLADRTGFEQLDLSLAARY
                     ADYEGSGGIWAWKVGLDWQIHDAVRLRTTSSRDIRAATLSERFDRQGVGSAVNDPAFG
                     GLNYTMTQIIGGNPNLNPEEADTVSFGLVWQPSFLEGFSMSADYYEIDIAGALAQVGV
                     QNIVDQCFSFGVMCDKVTRDTTTGRVTIVDNAFVNQDAARVVGTDIEAGYTHQLDGGG
                     TLSARLLATWLNENSTTARGAPKRDVAGETGDGSLPEFKSTTYLRYAKGPFSLFAQER
                     FIGSGSLDFDDVAGVTIDDNSVDSRYYTDMGLTWGPERANVQWQLFFNVQNLFDRDPP
                     PAASFAQFSGTRITNDRLFDFYGRRYVIGVDLTF"
     gene            complement(9345..>10179)
                     /locus_tag="VFL84_14465"
     CDS             complement(9345..>10179)
                     /locus_tag="VFL84_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7609881.1"
                     /translation="SSPTGGWMALRDGQPFAGINSIEDRIPGTAESLWLAAITVNDPK
                     TSVATAKRLGATIRQDVTELPGWGTYSVLQDPQGAPLLLVKPARSLGGTQGYSGWRWA
                     ELWTPDTKAAADFYTQVVGYELEAVPVGNETYDVFRTSAKRHAGLVGLDRADIAPRWA
                     PYIGVTDLRGILVRVWQAGGSVLREPAEVDFAAAGANRVALVADPSGAALFLYQLDER
                     ATADPGAIADARRASSTPPPPRPSGGDSNIRVSVTVGYGFGPGWGSAYPAYLRPTLGP
                     Y"
BASE COUNT         1615 a         3541 c         3392 g         1631 t
ORIGIN      
        1 gacggccgcc ggttggccgt tcagcgccgc gctcatgagc ggcgtctggc cttcacggga
       61 ccgtgcgttc ggatcggcgc ccgacgcgag cagccgctcg acgatctcgc cgttaccgtt
      121 gttcgcggcg agctcgagcg gcgtgacgcc gaagcggttc gccgcggtga cggctgcgcc
      181 cgcgtcgagc agcagctcga ccgtctcgag gtcgtcgcgc tcggcggccc agtgcagcgc
      241 cgtcgtgcca tcggcttccg cctcgtcgac ggccgcgccg cgatcgagca gcgcgctcgc
      301 cgccgcggcg tcttgccgct tcacggcatc gatgagcggc ccgcccgcgg cggcgagcgg
      361 cacgcaggcg actagcaccg tgacgatcga ccgacgcatg ccctctccac ccctggcgtt
      421 acgttgcctt cgaatatacg ccggccgcgc ggcgccggcc atgagcgggg ccccgtggca
      481 agcagcgcgc gaacgcccta ttctgctcga aaagaaccaa gcggcagggg catgaagcgc
      541 tacgtcgtcg gtatttccgt ctttgtcgtc acggccgccg ctgcagcgtg gatcgcgcgc
      601 cattcggacg aggccgcgac acgcggcctg ctcggccgtt actgcaccga ttgccacaac
      661 aagaccgact tcacggccga gctcgtcatc gagccgaccg acgtcgcgag catcgccgcc
      721 gatcccgagc actgggagaa gatcgtgcgc aagctgcgcg cggaagaaat gccgcccgac
      781 ggtccgcggc cgcagcacgc gcaataccag cgcgcggcgg cgttcctcgc gtcgcggctc
      841 gacgcggccg ccgcggcaga gccgcaaccg ggcgccgtgc cggcctttcg gcgcctgacg
      901 cgaaccgagt acaagaatgc gattcgcgat ttgctcgcgc tcgacggtct gccggcggag
      961 ctcgacttcg agctgctcct gccggccgac aacgcggcga gcggcttcga caacatcgcc
     1021 gatctgctgt tcgtctcccc cgtcgtcatg gagcgctaca tcgcggcggc gcagaagatc
     1081 gcgcggctcg ccgtaggcga tctgcgagcg cccgtcatgg tcaacatcca ccggctctcc
     1141 gagcagctgc cgcaggacga gcgcatcgac ggcctgtcgt tcggcacgcg cgggggctta
     1201 agggtcgaga cctacctgcc gctcgacgcc gagtacgtct tcgaggtcga gacggccgcc
     1261 cccgtccccg aggcgcacga gctcgaggtc agcgtcgacg gcgtgcgcgt cgcgaacgcg
     1321 acgctgctga cgcccgaacg tcggtcgggc gcgccgcggc gcagcaacga gcaggcgacg
     1381 tttcgcgtgc cgatcgcggc cggcccgcat ctgatcggca tcacgttcgt cgagcactcc
     1441 gaggcgctcg acgagagcat cgcgcgcggc cgcatgcgcg gccgcggcac gctgcccgcg
     1501 atcgcgatgg cgacgattcg cggcccctat gccgcgacgg gccccggcga tacgccgagc
     1561 cgcagccgca tcttcgtctg ccggccgaac ggcgcggccg acgagcccgc gtgcgcgcag
     1621 caaatcttgc gcacgctcgc gcacaaggcg tatcgccggc cggcgacgga cgccgacgtc
     1681 gccgacctca tgccgttcta cgaggcgggc cgcgccgagg gtaacttcga tacgggcatc
     1741 cagctcgcgc tcgagcgcct gctcgtgagc ccgcagttcc tctaccgcat cgagcgcgag
     1801 ccggccgagc ccggcgctca cttggtcagc gacatcgagc tcgcctcgcg cttgtcgttc
     1861 ttcctctgga gcagcatccc cgacgacgag ctgctggccg cggccgagag cggctcgctg
     1921 cgccggcccg gcgtcctgcg cgctcaggtg cagcgcatgc tggccgacgc tcgctccgag
     1981 gcgatggtca cgaacttcgc ggcgcagtgg ctgttcctgc gcgacgtcgc ggtcaaggac
     2041 cccgacatct tcctgtttcc ggaccacgac ccgtcgctgc gcgcggcgct cgagcgcgag
     2101 acggagctgt tcgtcggcag cgtgtttcgc agcggcggca gcgtgctcga tctcttgact
     2161 gcgaagcaca cgttcttgaa cgagcggctc gccaagcact acggggttcc gcacatccaa
     2221 ggcagctact tccgccgcgt cgagctcggg ggcgacagcc ggcgcgcggg cctgctcggg
     2281 caaggcagca tcctgaccgt cacgtcgtac gcggcgcgca cgtcgcccgt gctgcgcggc
     2341 aagtacgtgc tcgacaacct gctcgcgtcg ccgccgccgc ctccgccgcc cgacgtgccg
     2401 tcgctcgcga ccgaggacgc gaaggacggc gcgccgctga cgctgcgcga ggcgatgatc
     2461 cgccaccgcg cgaatcccgt gtgcgcggcc tgccacgcca agatggatcc gatcggcttt
     2521 gccctcgagc acttcgacgc cgtcggccgc tggcgcgacg aagatgccgg catgccgatc
     2581 gattcgagga gcgagctcgc cgacggccgc gtcatcgacg gcccggaggg cgtgcaggcg
     2641 ttcctgacgt cccggcccga gctcttcgtg agcgcgctca cggaaaaact gctgatgtat
     2701 gcgctaggaa ggaacgtgca gtattacgac gcgccggccg tccgggccgt cgtccgcgaa
     2761 gccgctgcac ggaactacga gttttcggct attgttgagg gcatcgtcgc aagcgtgccg
     2821 tttcagatgc gggcgaccgc cagggacggc gttgccgcga aggagaacga acagtgaagc
     2881 tcgaaacggg caagttcgta agccgcaaag ccctgccgcg ccgcacgttc ttgcgcggca
     2941 tcggagcgac gctcgcgctg ccgatgctcg acgcgatggt gccggcattc gccgccgacc
     3001 gcgcgccgtg gacgccgcgg ctcggcttcg tctacgtcgg caacggcatt gtgcacaaga
     3061 ccttcaagct cacgggcttg gggcgtgact tcgaattctc gcccgtgctg aagccgctcg
     3121 cgccgctgcg cgattacttg acggtcgtca gcggtctcga tcacgcgcaa gccaacaact
     3181 tcggcgacgg caccggcgat cacccgcggt cgtcggcggc ttggctcacg ggcgtgcacg
     3241 cctacgaccg cacgcagccc ggcgtcgagg tcaagctcac gacgacggcc gatcagctcg
     3301 ccgccgacgt cctcggcgcg catacgccgg ttcgctcgct cgagctcgcc gtcgacacgg
     3361 cgacgcagag cgcctgcgat tccggcgatt gcttctacgt gaacacggtt tcgtggcgca
     3421 atgagacgac gccgaacctg accgagaatc atccgcgcgt cgtcttcgag cgcctgttcg
     3481 gcgacggcgg caacgccgcg gaccgcagcg cccgcacgcg caagacggcg agcatcctcg
     3541 actcggtccg cgaggaagcg aaccgcctcg ccgcttcggt cggccacagc gatcgcagca
     3601 agctggccga gtatctcgac gccgtccgcg agatcgagca gcgcatcggc gcggccgagt
     3661 cggagacgca cggcgaggtg cagctgcccg agcgcccgct cggcattccg gagtcattcg
     3721 acgattacgc gaagctcatg ttggacctgc aactccttgc gtatcaatct gatacgacaa
     3781 gagttttcag catgatttta gcgcgcgagg tcagcagccg cccgtacccg cacatcggcg
     3841 tgccggacca gcatcatgcg gcttcgcacc accgcaacga tccgggcctg atcgagaaga
     3901 agaccaagat cgacgcctac catgcatcgc tgctcggcta tctcgccgag aagatgcgca
     3961 cgacgtccga cggcggcggc acgctgctcg atcagagctt gctcgtctac ggcggcggca
     4021 tgggcgacgg caatctgcac cagcacgtcg accttccctg cgtgctgctc ggcaagctcg
     4081 gcggcacagt aaaggccggc cagcacgtcg cgtacccaag cggtacgccg atgacgaatc
     4141 tgttgttgac ggtgctcgac aaggtcggcg tcactgtcgc cgcgatcggc gacagcacgg
     4201 ggcggctctc gcccgactat ctcacggcgg tctagcgcag ctggctgtcc ttgctgccgc
     4261 ggcggttgta gccgctgaag acgcgcacgt cggcgtcggc cttttgctga cagacgatgc
     4321 agagccgcac gccgggcagc gccgcacgcc gcgcctcggg aatcggctcg ccgcactcgt
     4381 ggcaatgcgt ggcgctcggt ccctgcggaa tgaggctctt cgcccgcgca actccgtcct
     4441 tgaccgtgtc gtctatctga tcctgtacgg cgccttcgcg cgcccaaccc gtagccatga
     4501 aaccgcctta ccgctcgaat acccgtcaga ggtaggttgc cgcaattggt tccctcgcgg
     4561 caatgcgcct gtacgcgctt ctgttcgtcg caaggtcccg gcaccgatct tgcacgaacc
     4621 cgagacgatg gcgaatgcac cacgcaagag gaccgccgag caaaaacccg acgcgctgga
     4681 cgaggccttc gacaagctgg agcaggcggt tcccgattcc gtcacggcgg cgattcgctg
     4741 gatgcgctca ccgcaggcgc gcatcgtgcg gttgccgctc ggaatcctgt gcatcctcgg
     4801 cagctttctt tggtttctac ccgtgctcgg cttgtggttc ctgccgctcg gtttgctgtt
     4861 gatcgcgcaa gacgtgccct tcatgcgcag gcccgtggga cgcatgaccc gctggttgct
     4921 cgatcgctgg gaacacttgc gccagtggtg gtcgcgcaaa aaggaagccc gcgcaaaacg
     4981 gctgcgcagc gaatctcgct gacgcgctga atggagccgt tgtggcatgc tgccgcgcat
     5041 gcgggttcgc agcagcacgc gtcagcagaa gaagaagtat cgaatcctgc tgatggtgca
     5101 cgagaccttc gtgccgcccg tcgaaaccgg catcggcgcg gcgcggctcg acgagattcg
     5161 caccgaggtc gacgtcctgc gcacgctgcg ccgcctcggt cacgaggtca tcgtcgtcgg
     5221 catcggcggc gatctcgcgg agcttcgcga gacgatcgac aagtggcggc cgcacgtcgt
     5281 gttcaatctg ctcgacgagt tcgcgggcat catctcgtac gacacccacg tcgtggccta
     5341 cctcgagctg ctcgagcagc gctacaccgg ctgcaatccg cgcggcatga tgttgtcgcg
     5401 cgacaaggtg ctcacgaagc gcgtgctcgc gacgcacggc atcacgacgc cggctttcaa
     5461 gctgttcccg ttcggccgcc gctttcacgc gccggcgcgc gtcaggttcc cgctgttcgt
     5521 gaagtcggcg accgaggacg cgtcgctcgg catcgcgcaa gcctcgctct gcgaggacat
     5581 gcgaagcctg cgcgagcgcg tcgagctgat ccacgaccag gtccagagcg acgcgctcgt
     5641 cgaggaatac atcgacggcc gcgagctcta cgtcggcgtg ctcggcaatg cgaagctcgt
     5701 gacgctgccg ccgtgggaga tggacttcgg cgccctgccg cgcgaccagg cgcgaatcgc
     5761 aacgcgcagg gcgaagtgga atcgccgcta tcaggcgaag cacggcatcg acaccggcaa
     5821 ggctcggcta cgcgacgacc agcacgccca cttgagccgc ctgtcgaagc ggatctaccg
     5881 cgcgctctac atgaccggct tcgcgcgcat ggactttcgc ctctgcagcg acggccgcct
     5941 ctttctgctc gaagccaact gcaatcccaa cctcagcaag ggcgaggact ttgcggattc
     6001 cgccaaggca gccggcatcg gctacacggc cctcgtcgac cgcatcctgc agctcgggct
     6061 cgcgtcgatg cctgaatggc gccagcacgc gccgcgagcc cgaaccgttc gtcagaacgt
     6121 caaatcgacg ccgatcacgt aacggcggcc gtagaaatcg aacagccgat cgttcgtgat
     6181 gcgcgtgccg ctgaactgcg cgaacgaggc cgccggcggc ggatcccggt cgaacaagtt
     6241 ctggacgttg aagaacagct gccactgcac gttcgcgcgc tcgggtcccc acgtgagccc
     6301 catgtcggtg tagtagcgcg agtcgacgga gttgtcgtcg atcgtgacgc ccgcgacgtc
     6361 gtcgaagtcg agcgacccgg agccgatgaa gcgctcctgg gcgaacaggc tgaagggccc
     6421 tttcgcgtag cgcagatacg tcgtcgactt gaactccggc agcgagccgt cgccggtttc
     6481 gcccgcgacg tcgcgcttcg gcgcgccgcg cgccgtcgtc gagttctcgt tgagccacgt
     6541 cgccaagagc cgcgccgata gcgtgccccc gccgtcgagc tgatgcgtgt aaccggcctc
     6601 gatgtccgtg ccgaccacgc gcgcggcgtc ctggttgacg aacgcgttgt cgacgatcgt
     6661 gacgcggcct gtcgtcgtgt cgcgcgtgac cttgtcgcac atgacgccga agctgaagca
     6721 ctggtcgacg atgttttgca cgccgacctg cgcgagcgcc ccggcgatgt cgatctcgta
     6781 gtaatcggcc gacatcgaga agccttcgag gaagctcggc tgccagacga ggccgaacga
     6841 gaccgtgtcg gcttcctcgg gattcaagtt cggattgccg ccgatgatct gcgtcatcgt
     6901 gtagttcagt ccgccgaacg ccgggtcgtt cacggcgctg ccgacgcctt ggcgatcgaa
     6961 ccgctcggac agcgtcgccg cgcggatgtc gcgcgacgac gtcgtgcgca agcgcacggc
     7021 gtcgtgaatc tgccagtcga ggcccacctt ccatgcccag attccgcccg agccttcgta
     7081 gtccgcgtaa cgcgccgcga gggagagatc gagctgctcg aaccccgtgc ggtcggcgag
     7141 cagcggcaac agcgtctcgg cgaacacctc cgagaccgaa tacgagcccg cgatcggctg
     7201 cacgttcgtg aagatgaaga tctcgttcgg cgtggccgcg aactgcgccg gcacgccgcg
     7261 gatgccggtg cagcccgccg gcggattcaa taggtcgacg gtgttgctga cgcagtccgg
     7321 cctcgtcgtc ggactgtccg ccgcgggaag cgagcccgcg atgatgtcgg cgggcccgag
     7381 cgtctggccg atcgattctt cgcgccaccc gccgccgaag gcgagcgaga tcgcgccggc
     7441 tccccagccg tcgccgacct cgccttccat cgagaattcg gcgacgttct gctccgtttc
     7501 cgcgaggatg tactggtcgc ccgtcagcac gtagtcgatc gcctgctgcg acgcgcggcc
     7561 tacgccgagc agattgatcg gcacgcagtc gccgtaacgc accggatcga agaacgacgc
     7621 gcggcacgcc ggctggcccg tcagcggatc ggccacgacg tccatcgcct cgggcagccg
     7681 gtcgctgcgg atgaagtcga cctgcgtgat gcggttgtcg ttcctcccga gctgcgcgta
     7741 accgccgagc gtccatcctt cgaacatgcc gccctgaagc tgcgactcgc cgccgaacgt
     7801 caacgatcga gtgtcgttgt cggtgacgaa ccgatcgtgc gcgagatcgg ccgtcgtgtg
     7861 gtagcgctcg agcaggaacg actggatgtt gttcgccgtc atgagctgct ggatgttggc
     7921 cggtagaaac ggattgccgc tgtagatcgt tcccgcccaa cccgcgatgc cgagaggcag
     7981 cgtgccgacg ctgttgacct tgttctcacc ggcgaggaac tgcgcgtaca acgacgcgtt
     8041 gtcggtgaga tcgaagttgt agtgcgcgaa caggctgccg cgctcggtct cgggcacgag
     8101 cgagccgccg cgatccgtga cggcgtccca gccgctgcct ccgttcgtaa tctggtgcgt
     8161 gccgtagctt ccgcccggcg tgctgacgcg cgggccggtc gccggattcg cgccgatgag
     8221 gttgccggca acgaacggtt gcgccgagcc gtccggcaga aactcgtagc ggtggatcgc
     8281 cgtcggaatg cggtcggcgc ccggtccggc gccgggcagc gtgctgttta tcaagccgcc
     8341 gttcgtgtac gtgtaggaca cgacgtcggg gcgaacgaga tcgcgcgggc ctcccgcgac
     8401 cgggttctgg atcaagcccc agtgccgaaa ccagtcgcgg ccttcgagcg tctcgatcct
     8461 gtcggcactg aaccattcgc cggacaccga caggcgaccg cgctcgccga ggctcgtgcc
     8521 gtacgcgacg gagacttcgc tgttctcgcc gtcgccgcgc tccgtcgcgc cgacctgcgt
     8581 gtggatgtcg aagccctcga aatcgtcctt caagatgaag ttcacgacgc ccgcgacggc
     8641 atcggtgccg tacacggccg acgcgccgcc cgtcacgacc tcgactcgct cgatcatcgc
     8701 ttcggggaac aagttgatgt cgacggcacc gagccgattc gaaggcacca tgcggcggcc
     8761 gtcgagcagc acgagcgtgc ggttcgcgcc caagccgcgc aggttcacgt tcgcggcgcc
     8821 ggccgattgc gacttgctcg ccgcggtgtc gggggcgtcg ttcatgaaga acggcggcag
     8881 ataatcgagc gagtcgatga actggcccgg cgccatcatc tgcatgtctt cggccgagat
     8941 agccgtgatc ggcgtcggcg agctcatgcc gtcgcgcgtg atgcgcgtgc ccgtcaccgt
     9001 gacctcgtcg attcccgacg gcccgggcgt cgcttcttgc gcggctgcga agcctgcgca
     9061 gagcgcaccg gcgacggcaa gcgaaagttt cgtgcgcgca agcgcacggc tgaatgactc
     9121 gattcgcgtc acgggatccc ccttgttgct cagtccattg cgcaacgggc ggtcgcggga
     9181 cacgccgtcg caaaagaacg atcagttgtt tgtcgtgagc ctagcgaacg gaaaaagccg
     9241 agtcaaaagg ctgacttggc ggttcggctg cgcgtttgcg cggatgcgac aaccgcagct
     9301 gccgcgagcg gccggagctt caatcgacgg cgcgccgcgc gcagtcagta cgggccgagc
     9361 gtcggccgca ggtaggccgg ataggcgctc ccccacccgg gcccgaagcc gtagccgacg
     9421 gtgacgctga cgcgaatgtt ggaatcgccg ccgctcgggc gcggcggcgg cggcgtcgag
     9481 ctcgcgcgcc gcgcgtcggc gatcgcgccg gggtccgcgg tcgcacgctc gtcgagctga
     9541 tagaggaaca gcgcggcgcc gctcggatcc gcgacgagcg cgacgcgatt cgcgccggcc
     9601 gcggcgaaat cgacctcggc cggctcccgc aacacgctgc cgccggcctg ccagacgcgc
     9661 acgaggatgc cgcgcaagtc cgtcacgccg atataaggcg cccagcgcgg cgcgatgtcc
     9721 gcgcggtcga gacccacgag cccggcgtgg cgcttcgccg aagtgcgaaa gacgtcgtac
     9781 gtctcgttgc cgaccgggac tgcctcgagc tcgtagccga cgacttgcgt gtaaaaatcc
     9841 gccgcggcct tcgtgtccgg cgtccacagc tccgcccagc gccagccgct atagccttgc
     9901 gttccgccga gcgatcgcgc cggcttcacg agcagcagcg gcgcgccctg cggatcttgc
     9961 agcacggaat aggtgcccca gccggggagc tcggtcacgt cttggcgaat cgtcgcgccg
    10021 agccgcttcg ccgtcgcgac ggacgtcttg ggatcgttga ccgtgatcgc ggcgagccac
    10081 agcgattcgg ccgtgcccgg aattcggtcc tcgatgctgt tgatgccggc gaacggctgg
    10141 ccgtcgcgca gcgccatcca gcccccggtc gggctcgac
//
LOCUS       DASSNN010000770         2041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1719189, whole genome shotgun sequence.
ACCESSION   DASSNN010000770 DASSNN010000000
VERSION     DASSNN010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719189"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VFL84_14470"
     CDS             <1..709
                     /locus_tag="VFL84_14470"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7609882.1"
                     /translation="DALGRQIQTASESGQEPIVMEIVGIAPGMPGPDFDSAPRQHLYV
                     PFGQAYTAGMSLLVAARANVADPVQLLAMIRDEIRDIDPALPVLSMMTMREYRNKNWS
                     LWFTRIGGQLFTVLGLVGLLMAMVGLYGVTAFLMSRRTREIGIRMALGATRGAVLRRF
                     MRESIRLTAAGIALGLLLALGAGRLLSSKLYGVSPRDPWVLLGASALLALAATAASWL
                     PVSRALRVAPSAALRDE"
     gene            complement(723..1640)
                     /locus_tag="VFL84_14475"
     CDS             complement(723..1640)
                     /locus_tag="VFL84_14475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7609883.1"
                     /translation="MAANEHQHRPVLGVFLKLSSVVLLSFLAACVKYLGDAVPTGQII
                     FVRGVISMAVLAFIAWQFHGFKILRTDRLRSHAIRSLAGTASMFCWFTALTMIPLADF
                     TAISFTAPLFLTLLAMLLLGERIHIYRWSALAIGFVGVIITIGPHLTFGGNSLGVLVA
                     FGAAVFSAAAIATLRSMSGAGGEHPLAITFYFSLTTVAVSALTAVGGWPMPTAEQWFF
                     IVISALFGVSGQLLMTMSYRYAEASTLAPLDYTNLLLAVTLGLYFFDEVPHWSMWIGA
                     PLVISAGLIILWREYRRYAAVPAPVVPND"
     gene            1733..>2041
                     /locus_tag="VFL84_14480"
     CDS             1733..>2041
                     /locus_tag="VFL84_14480"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7609884.1"
                     /translation="MRTTLITLSLASLTSFSVSSQAPNGEALFTQHCAACHLNPAEEK
                     IPTRAAMATLAPNAIVESLTDGTMRIQGQALSPPERVAIAELVTGRPVLAATSRLDQG
                     F"
BASE COUNT          387 a          655 c          688 g          311 t
ORIGIN      
        1 cgatgcgctc ggccggcaga ttcagaccgc ctccgagtcc gggcaggaac cgatcgtgat
       61 ggaaatcgtc ggcatcgcgc cgggcatgcc gggaccggac ttcgattcgg ccccgcgcca
      121 acacttgtac gtgccgttcg gccaagcgta cacggcgggc atgagcctgc tcgtcgcggc
      181 gcgtgcaaac gtcgccgatc cggtgcagct gctcgcgatg atccgcgacg agattcgcga
      241 catcgatccg gcgttaccgg tcttgtcgat gatgacgatg cgggagtacc gcaacaagaa
      301 ctggtcgttg tggttcacgc gaatcggcgg ccaactcttc acggtgctcg ggctcgtcgg
      361 tttgctcatg gcgatggtcg gtttgtacgg cgtcacggca ttcctaatgt cgcgccggac
      421 gcgagaaatc gggattcgca tggcgctcgg cgcgacgcgc ggcgcagtgc tccggcgatt
      481 catgcgcgaa agcattcgat tgacggcggc gggcatcgca ctggggctgc ttctcgcgct
      541 cggcgccggt cggctcctca gcagcaagct ctacggcgtc agcccccgag atccctgggt
      601 gctgctcggg gcgagcgcgc tactcgcgct cgcggcgacc gcagcatcgt ggttgccggt
      661 cagccgcgca ctgcgcgtcg cgccgagcgc ggcattgcgc gacgagtgag agctcgtgtt
      721 gctcagtcgt tcggcaccac gggcgccggc acggcagcgt agcgccggta ctcgcgccag
      781 agaatgatga gccccgccga gatgacgagc ggcgcgccga tccacatcga ccagtgcggc
      841 acctcgtcga agaagtagag cccgagcgtc acggcgagca gcaggttcgt atagtcgagc
      901 ggcgcgagcg tggaggcttc ggcgtagcga tacgacatcg tcatcaatag ctggccggac
      961 acgccgaaca aagcagagat gacgatgaag aaccactgct ccgccgtcgg catcggccaa
     1021 ccgccgacgg ccgtgagcgc cgaaacggct acggtcgtca gcgagaagta gaacgtgatc
     1081 gcaagcggat gctcgcctcc cgcgccgctc atgctgcgca gcgtcgcgat cgcggccgcc
     1141 gagaacaccg ccgcgccgaa cgcaacgagc acgccgagcg agttgccgcc gaacgtcagg
     1201 tgcggcccga tcgtgatgat gacaccgacg aagccgatcg caagcgcaga ccagcggtag
     1261 atatgaatgc gctcgccgag cagcagcatc gcgagcagcg tcaggaacag cggcgccgtg
     1321 aagctgatgg ccgtgaagtc ggcgagcggg atcatcgtca gcgcggtgaa ccagcagaac
     1381 atgctggccg ttccagccaa cgagcggatc gcgtgcgagc gcaggcggtc ggtgcgcagg
     1441 atcttgaaac cgtggaactg ccaagcgatg aacgcgagca cggccatcga gatcacaccg
     1501 cgcacgaaga tgatttgacc cgtcggcacg gcgtcgccca gatacttgac gcaggcggcg
     1561 agaaacgaga gcagcacgac cgaggacagc ttcaggaaga cgccgaggac cggccggtgt
     1621 tgatgttcgt tcgcggccaa ggcgcgcacc agtcgcgatg ctgacgggcg cacaccatag
     1681 caggccctac ggcgcttttt aagattcggc tattcttgcc gcgggaggac tcatgcgcac
     1741 caccttgatc acgttgtcgc tcgcttcgct gacgagcttc tcggtttcga gccaagcgcc
     1801 gaacggcgaa gcgctattca cacagcactg cgccgcgtgc catttgaacc cggccgagga
     1861 aaagattccg acgcgagccg cgatggctac gctcgcgccg aacgcgatcg tcgaatcgct
     1921 gaccgacggc acgatgcgca tccaagggca agcgttgtcg ccacccgagc gcgtcgcgat
     1981 cgccgagctc gtgacggggc gccccgtgct cgcggcgacg agtcggctcg accaaggctt
     2041 c
//
LOCUS       DASSNN010000771         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1339149, whole genome shotgun sequence.
ACCESSION   DASSNN010000771 DASSNN010000000
VERSION     DASSNN010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339149"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1549
                     /locus_tag="VFL84_14485"
     CDS             245..1549
                     /locus_tag="VFL84_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609885.1"
                     /translation="MQRKSVFDGLIGVGALALAVLLTGCEPGAAPGTTAAQPAPAGGA
                     ARRAGRNSYQDWPTGAYRVVEGWPKPLPDTRHAHAGWTWGSFGGVYAETPDRIWIAMR
                     GELPLPEGAAPWTPYVALNPSRGNATGNGDGITATCGEEPKRGWERRFEHSIIVVDRN
                     GNLVDEWPHLDPMFSQLPCGRGPHQIKISPYDPEKHVWIIDDQLHMIYRFTYDGKLEM
                     SKGEVGVRGRGPNTFDRPTDIAWLPDGTYFITDGYGGTRVAKYGPNDEFIKDWGGPPK
                     DPQNPGPNEFNTVHSIAISADQRLFVVDRGHERMQVFDTEGNFLDMWPLRSPHWPASQ
                     RTLVTNHFIDDKGFIWAGDAFTNRILKFDLDGNFLYSWGAPGPQPGRLGCSHGITTDQ
                     LGNLYLADCFAGRVQKFEPLPDADPDKIAGQIQRTWDTWKPN"
     gene            1628..>2007
                     /locus_tag="VFL84_14490"
     CDS             1628..>2007
                     /locus_tag="VFL84_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609886.1"
                     /translation="MPRAVLADLGDDLPPEGIGRGIRHVSYSDQGGRPDGVQVMYHRG
                     YLYVGHMFSDGVTVLDASDPRALKPVHFFTTAPNTRTHHLQVAEGHMLLANGANIVRM
                     QSYDSTRGYFENTLADSLTNRQPFR"
BASE COUNT          358 a          679 c          679 g          291 t
ORIGIN      
        1 tgatttgata gatcagcgct gcgcagccgc tcgcggcgaa cagcgcgagc aggacggcga
       61 gcgtcgcctt actcggatcg gcgacgccgg cgggcgatcg gggcgaagcg ggctcgggca
      121 cggcaagtga gcaagaccaa aggagcggtg attgcgccag caagccgagg cgtttgcaac
      181 cgcggctctc tcgtttttgc cgcacgcgcg gctcctggtg cagaatgccg cggggggtat
      241 gcatatgcag cggaagtccg tgttcgatgg gctcatcggc gtgggggctc tcgccttggc
      301 ggtgctgttg acgggttgcg agcctggagc tgcacccggc acgactgccg cgcagcctgc
      361 gccggccggc ggcgcggccc gtcgcgcagg ccgcaacagc tatcaagact ggccgacggg
      421 cgcataccgc gtcgtcgaag gctggccgaa gccgctgccc gatacgcgtc acgcgcacgc
      481 cggctggacc tggggctcgt tcggcggcgt ctacgcggaa acgcccgacc gcatctggat
      541 cgcgatgcgc ggcgagctgc cgttgcccga aggcgcggcg ccgtggacgc cgtacgtcgc
      601 gctgaatccg tcgcgcggca acgcgaccgg caacggcgac ggcatcaccg cgacgtgcgg
      661 cgaggagccg aagcgcggct gggagcgccg cttcgagcac tccatcatcg tcgtcgaccg
      721 caacggcaac ctcgtcgacg agtggccgca cttggacccg atgttcagcc agctgccgtg
      781 cggccgcggc ccgcatcaga tcaagatcag cccgtacgat cccgagaagc acgtctggat
      841 catcgacgac cagctgcaca tgatctatcg cttcacgtac gacggtaagc tcgagatgag
      901 caaaggcgag gtcggcgtgc gcggccgcgg gccgaacaca ttcgaccggc cgaccgacat
      961 cgcctggctg cccgacggca cctacttcat cacggacggt tacggcggca cgcgcgtcgc
     1021 gaagtacggc ccgaacgacg agttcatcaa ggactggggc ggaccgccga aggatccgca
     1081 gaacccgggc ccgaacgagt tcaacaccgt gcacagcatc gcgatcagcg cggatcaacg
     1141 gctgttcgtc gtcgaccgcg gccacgagcg catgcaggtg ttcgacaccg aaggcaattt
     1201 cctcgacatg tggccgctgc gctcgccgca ttggcccgcg agccagcgca cgctcgtgac
     1261 gaaccatttc atcgacgaca agggcttcat ctgggccggc gacgcgttca cgaatcgaat
     1321 cttgaagttc gatctcgacg gcaacttctt gtacagctgg ggcgcgccgg gcccgcagcc
     1381 cgggcggctc ggctgctcgc acgggatcac gacggatcag ctcggcaacc tgtatctcgc
     1441 ggattgcttc gccggccgag tccagaagtt cgagccgctg cctgacgccg acccggacaa
     1501 gatcgcgggg cagatacagc gcacgtggga cacgtggaag cctaactgag cgccgcgcgc
     1561 ggccgcccaa atgccgacgc ggcgtgcaca tctgaagacg ttgctcggcc tcgcggcgag
     1621 cggcgcgatg ccgcgcgcag tgctggccga cttgggcgac gacctccctc cggaagggat
     1681 cggccgcggc attcgtcacg tctcgtacag cgaccaaggc ggccggcccg acggcgtgca
     1741 ggtcatgtac caccgcggct atctgtacgt cggccacatg ttcagcgacg gcgtcacggt
     1801 gctcgacgcg agcgacccgc gcgcactgaa gccggtgcac ttcttcacga cggcgccgaa
     1861 cacgcgcacg caccacctgc aagtcgcgga agggcacatg ctgctcgcaa acggcgcgaa
     1921 catcgtgcgc atgcagtcgt acgacagcac gcgcggctac ttcgagaaca cgctcgccga
     1981 ttcgctgacg aaccggcagc cttttcg
//
LOCUS       DASSNN010000772         4704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1339220, whole genome shotgun sequence.
ACCESSION   DASSNN010000772 DASSNN010000000
VERSION     DASSNN010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339220"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VFL84_14495"
     CDS             <1..152
                     /locus_tag="VFL84_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET7609887.1"
                     /translation="IAMCGSGVTACHLLLALEAAGLGQGRLYAGSWSEWIRDPERPIR
                     TGAEP"
     gene            259..1506
                     /locus_tag="VFL84_14500"
     CDS             259..1506
                     /locus_tag="VFL84_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7609888.1"
                     /translation="MLRTSQIEASSLLKNVRYEIRGKLARRAHELERQGYEIIQLNIG
                     NPGLFGLRTPETMRLAMIENLPSAEAYCNQKGIFPAREAVVMQQQARGIDGVDADHVF
                     MGNGVSELIDLTLRGLLNPGDEVLIPSPDYPLWTAATVLNGGRAVYYPCPESQDFNPD
                     PDAVEKLVTPRTKAIVIINPNNPTGAVYSRERLAALAKLAERHGLVVMSDEIYDQMLY
                     DGAEFVPMATLARDTLCATFSGLSKIYRACGYRVGWVSFSGAVDRSERYMSALDLLAA
                     LRLCSNVPGQWAVQTALGGFQSIAKLCSPGGRLYQSRAAIIKGVAQSRYLSLVTPRGS
                     MYAFIGVDKQKLPGFDDEAFAMELLEQQHVLIAPGSSFNTPYRDHFRITTLPEEARLA
                     EVFQRIEHVLEQIAERPPRLETA"
     gene            1536..4058
                     /locus_tag="VFL84_14505"
     CDS             1536..4058
                     /locus_tag="VFL84_14505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET7609889.1"
                     /translation="MERSTTIVTSNALAANQWELHLSARAVAAGAAAWETPPVAPYAA
                     WLDELWLEHAGDRGPALSQNQSLALWRRVIAESAESSALIGHTGAAQWAAGAWNLLHR
                     WQIDPAMERAAPLEVDYRAFLSWCRAYRARLDGHGFVDRAEIEAALPSRVAALEGRLV
                     LADLDEPYPARDALLRELAARGAALETLAAPAAAGAPHAARLADAADELRAAFAWAAQ
                     RLAARPSSRVAIVVANAGRRAHEIERLAADLRPDECWIAGRTLADEPVIGAAVDALAL
                     AGAHASYAAFGRWLRSPFFAGAGEERFARARLDTELRAELRSQLSFRAAYRCGVADLL
                     AARVPASARALAAALDAVGAVARATPSRWGHIVTRFLAELGWQPPSARAVLLAWQSTL
                     DELARLTPIVGQISLDAALAELTRLLERSTRAALPVRGVHVLARVDEVGPGYDAVWVT
                     GFTDAAWPQPALGLPLLPLKLQREHGMPYSSPRDAQERSARAFERLVRRSPELVVSWP
                     ARVYDYETEPSPAIRDWPVLADGELAALTAARPPRAAARETRADTAPPLKATRVPGGT
                     GALGRQARCPLRAFYQDRLGARALEPLTFGVPARLRGIAAHRAVEQLLGDSPAQADLA
                     SKAGEVAPSVERALAKLFGRARPHLKALYELEAEQLERALAALLRAELTRAPFRVRAV
                     EQRATATIGPLTFDVRIDRVDELADGTLAIVDYKTSERATSGEWFGPRLRDAQVPLYA
                     SQSPERVGAAVVARLTPAEVRYFGFWPEGTFPGRASKAANPDTDAQLAVWRAQLAELA
                     GEIAAGDARVFADDYDDAAGAYAPLTRVFEQLALERGSAARW"
     gene            4064..>4704
                     /locus_tag="VFL84_14510"
     CDS             4064..>4704
                     /locus_tag="VFL84_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7609890.1"
                     /translation="MSDAEAREAALDPAESFIVQAPAGSGKTELLIQRYLALLATVRQ
                     PEQVVAITFTRKAAAEMRRRVLRALRLAAENTPSAEAHERTAFDLARAVIARDAEHDW
                     QLLAQPQRLRIDTLDAFNAWLARQLPVLAGGAAAADVVDKADDLYAQAARRCVEAVDG
                     DDAALERALRILLRDVGNDCAALEQLLAALLPRRDQWLRLLADGSSALRPLLEG"
BASE COUNT          627 a         1686 c         1707 g          684 t
ORIGIN      
        1 tcatcgcgat gtgcggctcg ggcgtcacgg cgtgccacct gctgctggcc ctcgaggcgg
       61 cggggctcgg gcaggggcgg ctctatgccg gctcctggag cgagtggatt cgggatcccg
      121 agcggccgat acggacggga gccgagccat gacggcccgc taactcttcg ctattgcgac
      181 gccggcgctt ggcccgggcc gcgcggcgtg gtaaagactt cagccaattc atcgagcgtc
      241 tgttcgaaag gaatcgtcgt gttgcgcacc tctcagatcg aagcgagctc tctgctcaag
      301 aacgtccgct atgagattcg cggcaagctc gcccggcgcg cccacgagct cgagcgacaa
      361 ggctacgaga tcatccagct caacatcggc aacccggggc tgttcggctt gcggacgccg
      421 gagacgatgc gcttggcgat gatcgagaac ctgccttcgg ccgaggccta ctgcaaccag
      481 aaaggcatct tcccggcgcg cgaggccgtc gtgatgcagc agcaggcgcg cggcatcgac
      541 ggcgtcgacg ccgatcacgt gttcatgggc aacggcgtca gcgagctgat cgatctcacg
      601 ctgcgcggcc tcttgaatcc cggcgacgag gttctgattc cgagccccga ctatccgctc
      661 tggacggcgg ccacggtctt gaacggcggc cgcgccgtct actacccgtg cccggaatcg
      721 caggacttca atccggaccc cgacgccgtc gagaagctcg tgacgccgcg gacgaaagcg
      781 atcgtcatca tcaatccgaa caacccgacg ggcgccgtct acagccgcga gcggctcgcg
      841 gcgctcgcga agctcgccga gcggcacggc ctcgtcgtca tgagcgacga gatctacgac
      901 cagatgctct acgacggcgc cgagttcgtg ccgatggcga cgctggcccg cgatacgttg
      961 tgcgcgacgt tcagcgggct ctcgaagatt tatcgcgcgt gcggctatcg cgtcggctgg
     1021 gtgtcgttca gcggcgccgt cgatcgctcg gaacgctaca tgagcgcgct cgacttgttg
     1081 gcggcgctgc gcctctgcag caacgtgcct gggcaatggg ccgtccagac tgcgctcggc
     1141 ggattccaga gcatcgcgaa gctctgctcg ccgggcggcc ggctctacca gtcgcgagcg
     1201 gccattatca aaggtgtcgc gcagagccgc tatttgagtc tcgtcacgcc gcgcggctcg
     1261 atgtacgcgt tcatcggcgt cgacaagcag aagctgcccg gcttcgacga cgaggcgttc
     1321 gcgatggagc tgctcgagca gcagcacgtg ctgatcgcgc cgggctcgag cttcaacacg
     1381 ccgtaccgcg accacttccg catcacgacg ttgcccgagg aagctcggct cgccgaagtg
     1441 ttccagcgca tcgagcacgt gctcgagcaa atcgccgagc ggccgccgcg cctcgaaacg
     1501 gcctaaaagt ggccgccgcg cacgcgagcg cgcgcgtgga gcgctcaact acgatcgtca
     1561 cttccaacgc tctcgccgcc aaccagtggg agctgcacct ctccgcgcgc gctgtcgcgg
     1621 ccggtgcggc ggcgtgggag acgcccccgg tggcgccgta tgcggcgtgg ctcgacgagc
     1681 tctggctcga gcatgccggc gaccgcggcc cggcgctgtc gcaaaatcaa tcgctggcgc
     1741 tgtggcgccg cgtcatcgcc gagtccgccg agagcagcgc tctgatcggg catacgggcg
     1801 ccgcgcagtg ggccgcaggc gcgtggaacc tgctgcaccg ctggcagatc gatccggcga
     1861 tggagcgagc cgcgccgctc gaggtcgact atcgcgcgtt cctgtcttgg tgccgcgctt
     1921 atcgcgcccg gctcgacggc cacggcttcg tcgaccgcgc cgagatcgaa gccgcgctgc
     1981 cgtctcgtgt cgcggcgctc gagggccggc tcgtgctcgc cgatctcgac gagccttacc
     2041 cggctcgcga cgcgctgctc cgcgagctcg ccgctcgcgg cgcggcgctc gagacgttgg
     2101 cggcgccggc ggcagccgga gccccgcacg cggcccggtt ggccgacgcc gccgacgagc
     2161 tgcgtgccgc gtttgcgtgg gcggcgcaac ggctcgccgc gcggccgtcg agccgcgtcg
     2221 cgatcgtcgt cgcgaacgcg ggccggcgcg cccacgagat cgagcggctc gcggccgact
     2281 tgcggcccga cgagtgctgg atcgcgggac gcacgctggc cgacgagccc gtgatcggcg
     2341 cggccgtcga cgcgctcgcg ctcgcgggcg cccacgcttc gtatgcggcc ttcggccggt
     2401 ggctgcgcag cccgttcttc gcgggcgccg gcgaggaacg cttcgcgagg gcgcggctcg
     2461 acacggagct gcgagccgag ctgcgctcgc agctttcgtt ccgcgccgcc taccgctgcg
     2521 gcgtcgccga cctgctcgcg gcgcgcgtgc cggcgtccgc gcgcgcgctc gcggccgcgc
     2581 tcgacgcggt cggcgccgtt gcgcgcgcga cgccgagccg gtggggccac atcgtcacgc
     2641 ggttcctggc cgagcttggc tggcagccgc cgtcggcgcg agccgtgctg ctcgcgtggc
     2701 agagcacgct cgacgagctc gcgcggctga cgccgatcgt cgggcagatc tcgctcgacg
     2761 cggcactcgc ggagcttacg cgcctgctcg agcgctcgac gcgtgccgcg ctgccggttc
     2821 gcggcgtgca cgtgctcgcg cgcgtcgacg aggtcggccc gggttacgac gccgtctggg
     2881 tgacgggatt cacggacgcc gcgtggccgc agccggcgct cgggctaccg ttgctgccgc
     2941 tgaagctgca gcgcgagcac ggcatgccgt attcgtcgcc gcgcgacgcg caagagcgct
     3001 cggcgcgcgc gttcgagcgc ctcgttcgcc gcagccccga gctcgtcgtc agctggccgg
     3061 cgcgcgtcta cgactacgag acggagccga gtcccgcgat tcgcgattgg cccgtgctcg
     3121 cggacggcga gctcgcggcg ctgaccgcgg cgcggccgcc gcgcgcggcg gctcgcgaga
     3181 cgcgtgccga caccgcgccg ccgctcaaag cgacgcgtgt gcccggcggg accggcgctc
     3241 tcggccgcca agcgcgctgc ccgctgcgcg cgttctatca ggatcggctc ggcgctcggg
     3301 cgctcgagcc gctcacgttc ggcgtgccgg cgcggctgcg cggcatcgcg gcgcatcgcg
     3361 ccgtggagca gctgctcggc gattcgccgg cgcaggccga cttggcgagc aaggccggcg
     3421 aggttgcgcc gagcgtggag cgcgcgctcg cgaagctgtt cggacgagcg cggccgcatc
     3481 tcaaggcgct gtacgagctc gaagccgagc agctcgagcg cgcgctcgca gcgctgctgc
     3541 gcgcggagct cacgcgcgcg ccgtttcgcg tgcgcgcagt cgagcagcgc gcgacggcga
     3601 cgatcgggcc gttgacgttc gacgtccgca tcgaccgcgt cgacgagctc gccgacggca
     3661 cgctcgcgat cgtcgactac aagaccagcg agcgcgcgac gagcggcgaa tggttcggcc
     3721 cgcggttgcg cgacgcgcag gtgccgctct acgcgagcca atcgcccgag cgcgtgggcg
     3781 cggccgtcgt cgcgcggctc acgccggccg aagtgcgcta cttcggtttc tggccggagg
     3841 gcaccttccc ggggcgcgcg tcaaaggccg cgaatcccga caccgacgcg cagctcgccg
     3901 tgtggcgcgc gcagctcgcg gagctggccg gcgagatcgc ggccggcgac gcgcgggtct
     3961 tcgccgacga ctacgacgac gcggcgggcg cttacgcgcc gttgacgcgc gtcttcgagc
     4021 agctcgcgct cgagcgcggc tcggcggctc gctggtgagc gcgatgagcg acgcagaggc
     4081 gcgcgaggcg gcgctcgatc ccgcggagtc gttcatcgtt caggcgccgg ctggctccgg
     4141 caagaccgag cttttgatcc agcgctactt ggcgctgctc gcgacggtgc ggcagcccga
     4201 gcaggtcgtc gcgatcacgt tcacgcgcaa agcggccgcc gagatgcgcc ggcgcgtgct
     4261 gcgcgcgctg cgcctcgcgg ccgagaacac gccgagcgcg gaggcgcacg agcgcacggc
     4321 attcgacttg gcgcgcgcgg tgatcgcgcg cgacgccgag cacgactggc agctgctcgc
     4381 gcagccgcag cgcttgcgca tcgacacgct cgacgcattc aacgcgtggc tcgctcggca
     4441 actgccggtg ctcgccggcg gcgccgcggc ggccgacgtc gtcgacaaag ccgatgacct
     4501 ctacgcgcaa gccgcgcgcc gctgcgtcga agcagtcgac ggcgacgatg cggcgctcga
     4561 gcgcgcgctg cgaattttgc tccgcgacgt cggcaacgat tgcgcggcgc tcgagcagtt
     4621 gctcgcggct ctgctgccgc gccgcgacca atggctgcgg ttgctcgcgg acgggtcgag
     4681 cgcgcttcgc ccgctgctcg aggg
//
LOCUS       DASSNN010000773         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1884589, whole genome shotgun sequence.
ACCESSION   DASSNN010000773 DASSNN010000000
VERSION     DASSNN010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884589"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VFL84_14515"
     CDS             complement(<1..174)
                     /locus_tag="VFL84_14515"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7609891.1"
                     /translation="MAQTNPPFLTGVPELLVLRMLSGREMYGYEIVKAMRLVTAEAIG
                     FGEGVIYPVLHSLE"
     gene            complement(277..>2092)
                     /locus_tag="VFL84_14520"
     CDS             complement(277..>2092)
                     /locus_tag="VFL84_14520"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7609892.1"
                     /translation="KVPGAAGAKACCDVNSRGPAVYENKLIIATLDGRLIALDRATGA
                     PVWSTVTVDQSKMYTITGAPRVVKNKVVIGNAGAEFGVRGYVSAYDADTGELAWRFHV
                     VPGDPNAGPDGAASDKALAELAAPTWFGRWYEMGGGGTPWDAIVYDPELDQLYVGTGN
                     GSPWNRQIRSDGRGDNLFLSSVLALDPDTGAYLWHYQESPGESWDFTSTQPMILADLD
                     VGGAARKVILHAPKNGFFYVLDRQTGKVLAANNFVPTYWATHVDLATGRPAIVSNAYY
                     DSGPFVGSPTGLGAHNWSPMSYSPLTGLVYFRASRASMIYRHDPDFVFREGLNNTGIY
                     RGPPTDEDRAINEANPPLSGSSLLAWDPVAGAPRFTIDGFGGGVLTTAANLLFQGRGT
                     ITGELVAFDATTGERLWSHETPNSMAPGPISYSVGGEQYIAVMGGRGFSGSNDADARA
                     RNPGRIFAFKLDGKAMLPPGPGPAPPPNVPDEIFSTAAVAAGAEPYRVYCSRCHGGET
                     HSLNIIPDLRRSAALSSAALWRSIVVDGALEPNGMIGWSQFLTPEEAETIRAYVAAQA
                     RMLQQVEAPESAVRPLGIEPPTIEPPVIDPAAVDPTAL"
BASE COUNT          329 a          686 c          725 g          352 t
ORIGIN      
        1 ctcgagcgaa tgcagcacgg gatagatcac gccttcgccg aaaccgatgg cctcggccgt
       61 gacgagcctc atcgccttga cgatttcgta cccgtacatc tcgcgcccgc tcaacatccg
      121 cagcacgagc agctccggca cgccggtcaa gaatggaggg ttcgtctgtg ccataactta
      181 gaacgataga ctcaacttgg tgtactggcc agtccgatct agcggtttga tcgagctgaa
      241 atttcgcgct cggacttgac tccggacgac gcgaagttat agggcggtcg ggtcgacggc
      301 tgcggggtcg atgacgggcg gctcgatcgt cggcggctcg atgcccaagg ggcgcacggc
      361 cgattcgggc gcttccactt gttgcagcat ccgcgcctgc gccgccacgt aagcgcggat
      421 cgtctccgct tcctcgggcg tcaagaactg cgaccagccg atcatgccgt tcggctcgag
      481 cgcgccgtcg acgacgatgc tgcgccaaag cgcggcgctc gagagcgcgg ccgagcggcg
      541 cagatccggg atgatgttca gcgagtgcgt ctcgccgccg tgacagcgcg agcaatacac
      601 gcgatagggt tcggcgccgg ccgcgacggc ggcggtcgag aagatctcgt cgggcacgtt
      661 cggcggcgga gcgggcccgg gccccggcgg cagcatcgct ttgccgtcga gcttgaaggc
      721 gaatatgcgc ccagggttgc gggcgcgcgc gtcggcgtcg ttactgcccg agaatcctcg
      781 ccctcccatg actgcgatgt actgctcgcc gccgaccgag tagctgatcg ggcccggcgc
      841 catcgagttc ggcgtctcgt gcgaccacag ccgttcgccg gtcgtcgcgt cgaacgcgac
      901 gagctcgccc gtgatcgtgc cgcgcccttg aaacaataga ttcgcggccg tcgtcaaaac
      961 gccgccgccg aagccgtcga tcgtgaagcg cggcgccccc gcgacggggt cccacgcgag
     1021 caacgacgag cccgagagcg gcgggttcgc ctcgttgatg gcgcggtcct cgtcggtcgg
     1081 cgggccgcgg tagatgccgg tgttgttgag tccctcgcga aatacgaagt cgggatcgtg
     1141 gcggtagatc atcgaagcgc gcgacgcgcg aaagtagacg agccccgtga gggggctgta
     1201 cgacatcggc gaccagttgt gcgcgcccag gcccgtcggc gagccgacga acggcccgga
     1261 gtcgtagtac gcgttcgaga cgattgcggg gcggcccgtc gcgaggtcga cgtgcgtagc
     1321 ccaatacgtc ggcacgaagt tgtttgccgc gagcactttg cccgtttgcc gatcgagcac
     1381 gtagaagaag ccgttcttcg gcgcgtgcag gatgactttg cgcgcggcgc cgcctacgtc
     1441 caagtcggcg aggatcatcg gttgcgtcga tgtaaagtcc cacgactcgc cgggcgattc
     1501 ttggtagtgc cacaggtatg cgccggtgtc ggggtcgagc gcgagcacgc tcgagagaaa
     1561 caggttgtcg ccgcggccgt cggacctgat ttgccgattc cacgggctgc cgttgccggt
     1621 gccgacgtag agctgatcga gctcggggtc gtagacgatc gcgtcccacg gcgtgccgcc
     1681 gccacccatc tcgtaccagc ggccgaacca cgtcggcgcc gcgagctcgg cgagcgcctt
     1741 gtccgacgcg gcgccgtcgg ggccggcgtt cggatcgccg ggcacgacgt ggaagcgcca
     1801 cgcgagctcg cccgtatcgg cgtcgtacgc gctgacgtag ccgcgcacgc cgaactccgc
     1861 gccggcgttg ccgatgacga ccttgttctt tacgacgcgc ggagcccccg tgatcgtgta
     1921 catcttcgat tgatcgacgg tgaccgtgct ccagaccggc gcgcccgtcg cgcgatcgag
     1981 cgcgatgagc cggccgtcga gcgtcgcgat gatgagcttg ttctcgtaga cggcggggcc
     2041 gcgcgagttc acgtcgcagc acgccttcgc tccggccgcg ccgggcactt tc
//
LOCUS       DASSNN010000774         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1884613, whole genome shotgun sequence.
ACCESSION   DASSNN010000774 DASSNN010000000
VERSION     DASSNN010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884613"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFL84_14525"
     CDS             <1..253
                     /locus_tag="VFL84_14525"
                     /inference="COORDINATES: protein motif:HMM:NF027650.4"
                     /note="Uup; in Escherichia coli this cytoplasmic protein
                     was shown to contain ATPase activity; mutations in this
                     gene affect RecA-independent excision of transposons and
                     affects Mu bacteriophage growth; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7609893.1"
                     /translation="PPPRKLSYKLQRELDALPDLIAGLEQKVAELRAVVNEPQFYRRP
                     HREVQDALAALHGAEREVETAIDRWAELEEQAAAAGDGG"
     gene            complement(250..1485)
                     /gene="dinB"
                     /locus_tag="VFL84_14530"
     CDS             complement(250..1485)
                     /gene="dinB"
                     /locus_tag="VFL84_14530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET7609894.1"
                     /translation="MSEDWPRIIVHADMDAFYAAVEQLDDPSLRGRPLLVGSDSARGV
                     VLTASYEARVSGAGSAMPMHRARRLCPEALVVPPRFERYQEISELVMKAFGDFSPVVE
                     PLSLDEAFLDMTGSTRLFGDPKSIGLRIKDAVRECTGGLTASVGISATRYVAKVASGF
                     RKPDGLTIVPPASMREWLAPQPVANLWGAGPKTAERLRASGFETIGQVASADAAALER
                     AFGALGRRFLALANGVDPREVVGSRRAHRVSSERTLNVDIAKPAQIAGYLRLSAANVA
                     ARLRRSHRFARGVRVKLKTSDFRVVTRQRVVEPTDVGAVLFSHATSLLAELAGESGPF
                     RLVGLAAYDLVGAEALAAQLGLLPEAGNRERRLETALDALAARFGRGVVQRAGDLARD
                     RGVGVAANLDFLHDRENDD"
     gene            complement(1482..2378)
                     /locus_tag="VFL84_14535"
     CDS             complement(1482..2378)
                     /locus_tag="VFL84_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET7609895.1"
                     /translation="MPELPDITLYIEALRQRLLGQRLERSRVRSPFLLRSVDPPLEAV
                     QGARVAAFERLGKRIAIGFDNDVWLVLHLMIAGRLHWRDDWPAKPTRQTALALGFENG
                     TLLLTEAGTKKRASLHVARGRAGLAAHQPGGLELDRASAAEFEAALKRGNHTLKRALT
                     DPRLISGVGNAYSDEILHRARLSPVTLTSRLTSEEAARLFAAAREVLAAWLKRLRDKA
                     HGDFPGKVTAFHDEMAVHGKYGKPCPVCQTKVQRIRYADNETNYCPRCQTNGRLLADR
                     SLSRLLKKDWPKSIDELEQLRP"
     gene            complement(2383..>2812)
                     /locus_tag="VFL84_14540"
     CDS             complement(2383..>2812)
                     /locus_tag="VFL84_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609896.1"
                     /translation="EEVAQVVQAQLAELGGAATQAAGPAPVGPEIKLSVTLGEGRSLT
                     ALAPTAQLFVMALAPEGAGPPLAVIRRPPSAVPGEFSLSDANSMIQGRSLAAYPEITV
                     VARLSRSGQPTAQPGDWQAEAVVRPAEAPTVALVIDQVVQ"
BASE COUNT          421 a          995 c          986 g          410 t
ORIGIN      
        1 gccgccgccg cgcaagctct cgtacaagct gcagcgcgag ctcgacgccc tgcccgatct
       61 gatcgcgggg ctcgagcaga aagtcgcgga gctgcgcgcc gtcgtcaacg agccgcagtt
      121 ctatcggcgg ccgcatcgcg aggttcagga cgcgctcgct gcgctgcacg gtgccgagcg
      181 cgaggtcgaa accgcgatcg atcgctgggc cgagctcgag gagcaagcgg cggccgcggg
      241 cgacggcggt tagtcgtcgt tttcgcggtc gtgcaggaag tcgagattcg cggcgacccc
      301 gacgccgcgg tcgcgcgcga gatcgccggc gcgctgcacg acgccgcggc cgaatcgagc
      361 cgcgagcgcg tcgagcgcgg tctcgagccg ccgctcgcgg ttcccggctt cgggcaggag
      421 cccgagctgc gcggccagcg cctcggcgcc gaccagatcg taggcggcaa ggccgacgag
      481 acggaagggg ccgctctccc ccgccagttc ggcaagcagc gacgtagcgt gcgagaacag
      541 cacggcgccg acgtcggtcg gctccacgac gcgttgccga gtcacgacgc gaaagtccga
      601 ggtcttgagc ttcacgcgca cgccgcgcgc gaaccggtgc gatctgcgca gtcgcgcggc
      661 gacgttcgcc gcggacaagc gtaggtaccc tgcgatttgg gccggcttcg cgatgtcgac
      721 gttcaacgtg cgctccgagc tcaccctgtg cgcgcgacgc gagccgacga cctcgcgcgg
      781 atcgacgccg ttcgcgagcg cgaggaaacg ccggccgagc gcgccgaacg cgcgctcgag
      841 agccgcggcg tccgcgctcg cgacctggcc gatcgtctcg aagcccgacg cgcgcaggcg
      901 ctcggcggtc tttggaccgg cgccccaaag attcgccacg ggctgcggcg cgagccactc
      961 gcgcatgctc gccggcggca cgatcgtgag cccgtcgggc ttgcgaaagc cgctcgcgac
     1021 tttcgcgacg tagcgcgtcg ccgaaattcc gaccgacgcc gtcaggccgc ccgtgcattc
     1081 gcgcacggcg tctttgatcc gcaggccgat cgacttgggg tcgccgaaca gccgcgtgct
     1141 gcccgtcatg tcgaggaacg cctcgtcgag gctcagcggc tcgacgaccg gcgagaaatc
     1201 gccgaacgcc ttcatcacga gctccgagat ctcctgatag cgctcgaagc gcggcggcac
     1261 gacgagcgcc tcggggcaca accgccgcgc gcggtgcatc ggcatcgcgc tgccggcgcc
     1321 ggacacgcga gcttcgtagc tcgcggtcaa cacgacgccg cgagcgctgt ccgatcccac
     1381 gagcagcggc cgccctcgta agctcggatc gtcgagctgc tcgacggccg cataaaacgc
     1441 gtccatgtcg gcgtgcacga tgatgcgcgg ccagtcctcg ctcatggccg cagttgctcg
     1501 agctcgtcga tcgatttcgg ccagtccttc ttcagcaacc gcgacagcga ccgatcggcc
     1561 agcaagcgcc cgttcgtctg gcaccgcggg cagtagttcg tctcgttgtc ggcatagcgg
     1621 atgcgctgca ctttggtctg gcacacggga cacggcttgc cgtacttgcc gtggacggcc
     1681 atctcgtcgt gaaacgccgt gaccttgccg gggaagtcgc cgtgcgcttt gtcgcgcaag
     1741 cgcttgagcc acgccgcgag cacctcgcgc gcggccgcga agagccgcgc cgcttcctcc
     1801 gacgtcaagc ggctcgtcaa agtgaccggc gacagtcgtg cgcggtgcag gatctcgtcg
     1861 gagtacgcgt tgccgacacc gctgatcaag cgtggatcgg tcagcgcccg tttcaacgtg
     1921 tgattgccgc gcttcagcgc cgcttcgaac tccgcggcgc tcgcgcgatc gagctcgagg
     1981 ccgccgggct ggtgcgccgc gagccccgcg cgcccgcgcg caacgtgcag cgaggcgcgc
     2041 ttcttcgtgc cggcttccgt cagaagcaac gtgccgttct cgaagccgag cgccaatgcg
     2101 gtctggcgcg tcggcttcgc gggccagtcg tctcgccagt gcagccgccc cgcgatcatg
     2161 aggtgaagca cgagccagac gtcgttgtcg aagccgatcg cgatgcgctt gccgaggcgc
     2221 tcgaatgcgg cgacgcgcgc gccctgcacc gcctcgagcg gcggatcgac gctgcgcagc
     2281 agaaacggcg aacggacgcg gctgcgctct agacgctggc cgagaaggcg ctgccgcaac
     2341 gcttcgatgt agagcgtgat gtcgggcagc tccggcatgc gctcattgga ccacctgatc
     2401 gatgacgagc gctacggtgg gcgcttcggc cggacgcacg acggcctcgg cctgccaatc
     2461 gccgggctgc gccgtcggtt ggccggagcg cgacaaccgt gcgacgacgg tgatctcagg
     2521 ataggccgcg agcgagcggc cctggatcat gctattcgcg tcgctcagcg agaactcgcc
     2581 gggcactgcg ctcggagggc gacggatgac cgcgagcggc ggtccggcac cttcgggcgc
     2641 gagggccatg acgaacagct gagcggtcgg cgcgagcgcc gtcagcgagc gtccctcgcc
     2701 gagcgtgacg ctgagcttga tctcgggccc gacgggcgcc ggcccggccg cctgcgtcgc
     2761 tgctccaccg agctccgcga gctgtgcctg gacgacttgc gccacttcct ct
//
LOCUS       DASSNN010000775         3013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_998973, whole genome shotgun sequence.
ACCESSION   DASSNN010000775 DASSNN010000000
VERSION     DASSNN010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998973"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..525
                     /locus_tag="VFL84_14545"
     CDS             52..525
                     /locus_tag="VFL84_14545"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HET7609897.1"
                     /translation="MEFLVWLESTPIANAIRTIPWLYPTFETGHYIGLSLLVGGIMLI
                     DLRVLGFARRLPLKSMIGLLPFVWAGFVINVLTGSMLFIYGATNFGTNKAFMLKMAFM
                     VVAGLNALAFDLSVRRSRGSWVAADRPPALVKGFATASLVFWVCVVTTGRWMAYL"
     gene            671..1492
                     /locus_tag="VFL84_14550"
     CDS             671..1492
                     /locus_tag="VFL84_14550"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012595452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET7609898.1"
                     /translation="MPMGFPVAGFDSGQRYRAAPGDIFVASYPKCGTTWTQYIVYLLE
                     NGGRPLAAGERLDAVFPHLEEVGEEIVRALRAPRLIKTHLPFSRAPWSTQARYVYVAR
                     NPFDCAVSFYHHTRGFPRHYDFAEGTWDTFFECFMRGEVDFGGYFEHLGSWWPHRSEP
                     NVLFLTYERMVEAPAAAIRAIAEFLGGRPRELARDALALDGVVRESGFKEMRRDQDRW
                     SSARPADMPAFVRRGVVGDWRNQFSAEQARRLADKCTGLPAIDELWPGLAANALE"
     gene            1510..1842
                     /locus_tag="VFL84_14555"
     CDS             1510..1842
                     /locus_tag="VFL84_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002540963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HET7609899.1"
                     /translation="MTDKLTERQETELEAAVLRRLLRHFDEHKEVQNIELMTLAGFCR
                     NCLSKWFVEEAKQRGLTVDLESAREKIYGMPYSEWKERYQPPATEEQLARLAAVEKAK
                     KEKAGGKA"
     gene            complement(1839..2840)
                     /gene="ispB"
                     /locus_tag="VFL84_14560"
     CDS             complement(1839..2840)
                     /gene="ispB"
                     /locus_tag="VFL84_14560"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HET7609900.1"
                     /translation="MSLVKPNPEGPSLEALRALVGDDWADVNRMIVRRLGSDVALVNQ
                     VAHHIIHNGGKRLRPLAVLLAARACGAGRSQDHVAAAAIIEFIHTATLLHDDVVDSSG
                     LRRGQETANAVFGNQASVLVGDFLYSRSFQMMVELKEMRILDVLANATNTIAEGEVLQ
                     LMNCNNPDIGEADYMEVIYRKTAKLFEAGTRIGAILAKQSDEVEQALATYGRHLGQAF
                     QLVDDALDYDAKVEELGKNIGDDLAEGKPTLPLIYAMAHGSAAERHMIREAIETGGTR
                     NLTSIQLAIETSGGLRYTAERARSEVELAIAALAPLPASKFRDGLVDLAQFSIQRRY"
BASE COUNT          500 a          985 c          987 g          541 t
ORIGIN      
        1 gcttgatcgc gtacgcgccg tgatgcgcgc gctgcgccga ggctaaagcc gatggaattc
       61 ctggtctggc tcgagagtac gccgatcgcg aacgcgatcc gcacgatccc gtggctctac
      121 ccgacgttcg agacgggcca ctacatcggc ctgtcgctgc tcgtcggcgg catcatgctg
      181 atcgacctgc gcgtgctcgg gttcgcgaga aggctgccgc tcaagagcat gatcggcctc
      241 ttaccgttcg tctgggcggg cttcgtcatc aacgtactca cgggctcaat gctgttcatt
      301 tacggcgcga cgaacttcgg cacgaacaag gcgttcatgt tgaagatggc gttcatggtc
      361 gtcgccggct tgaacgcgct cgcgttcgac ctgtccgtgc gccgttcacg cgggtcctgg
      421 gtcgcggcag accgcccgcc ggcgctcgtg aagggcttcg cgaccgcgtc gctcgttttc
      481 tgggtctgcg tcgtcacgac aggccgatgg atggcctatc tctaatgcac acctctaaat
      541 ggggacagat ctatttttgt ctccaacggg gacggacccc atttcgcccg aaaaatagat
      601 ctgtcccctt ttaggcgtgg gcgactcgac gggtcgcggt ccgcgttaca cgatccacga
      661 cggctttcgc atgccgatgg gctttcccgt cgcgggcttc gactcgggcc agcgttatcg
      721 tgcagcaccg ggcgacattt tcgtcgcttc gtatccgaaa tgcggcacga cgtggacgca
      781 gtacatcgtc tacttgctcg agaacggcgg ccggcccctt gccgccggag aacggctcga
      841 cgccgtgttc ccgcatctcg aggaagtggg cgaggagatc gtgcgtgcgc tgcgcgctcc
      901 aaggctcatc aaaacgcatc tgccgttctc gcgcgcgccg tggagcacgc aggcgcgata
      961 tgtctacgtc gcgcgcaatc cgttcgactg cgccgtctcg ttctatcacc acacgcgcgg
     1021 tttcccgcgt cactacgact tcgccgaagg cacgtgggac acgttcttcg agtgcttcat
     1081 gcggggcgag gtcgatttcg gcggctactt cgagcacttg ggctcgtggt ggccgcatcg
     1141 ctctgagccg aacgttctgt tcctgaccta cgagcgaatg gtggaggcgc ctgcggccgc
     1201 gattcgcgcg atcgccgaat tcttgggcgg acgcccgcgc gagcttgcgc gcgacgcgct
     1261 tgcgctcgac ggcgtcgtgc gcgagagcgg tttcaaggaa atgcgccgcg accaggaccg
     1321 ctggtcgagc gcgcgccccg ccgacatgcc ggcgttcgtg cgcagaggcg tcgtcggcga
     1381 ctggcgcaat cagttctcgg ccgagcaagc gcggcgcctc gccgacaaat gcacgggcct
     1441 gcccgcgatc gacgagctct ggcccggtct cgccgcaaac gcgctagagt agagccgcga
     1501 ggaaaagcca tgacagacaa gctaactgag cgccaagaga ccgagctcga agccgccgtg
     1561 ctgcggcgcc tgctgcgcca cttcgacgag cacaaggaag tgcagaacat cgaattgatg
     1621 acgctcgccg gtttctgccg caattgcttg agcaagtggt tcgtcgagga agccaagcaa
     1681 cgcgggctta ccgtcgatct cgagagcgcg cgcgaaaaga tctacggcat gccgtactcg
     1741 gagtggaaag agcgctatca gccgcccgcg accgaggagc agctcgcgcg gctcgccgcc
     1801 gtcgagaagg caaagaaaga aaaagccggc ggcaaggcct aatagcgccg ctggatcgag
     1861 aactgcgcga ggtcgacgag cccgtcgcgg aacttcgagg ccggaagcgg cgccagcgcc
     1921 gcgatcgcga gctcgacttc cgagcgagcg cgctcggccg tgtaccgcaa gccgcccgac
     1981 gtctcgatcg cgagctggat cgaggtcaag ttgcgcgtcc cgccggtctc gatcgcctca
     2041 cggatcatgt ggcgctcggc cgcgctgccg tgcgccatgg catagatgag cggcagcgtc
     2101 ggcttgcctt cggcgaggtc gtcgccgatg ttcttgccga gctcctcgac tttggcgtcg
     2161 tagtcgagcg cgtcgtcgac gagctggaac gcttgaccga gatgccggcc gtacgtcgcg
     2221 agcgcctgct cgacctcgtc gctctgcttc gcgaggatcg cgccgatgcg cgtgcccgcc
     2281 tcgaacagct tcgccgtctt gcggtagatg acctccatgt agtcggcctc gccgatgtcg
     2341 gggttgttgc agttcatgag ctgcagcacc tcgccctcgg cgatcgtgtt cgtcgcattc
     2401 gcgagcacgt cgagaatgcg catttccttg agctcgacca tcatctggaa cgagcgcgaa
     2461 tagaggaaat cgccgacgag cacgctcgcc tgattgccga acacggcgtt cgcggtctcc
     2521 tggccgcggc gcaggcccga gctgtcgacg acgtcgtcgt gcagcagcgt cgcggtatgg
     2581 atgaactcga tgatggccgc ggcggcgacg tggtcttggc tgcggccggc gccgcaggcg
     2641 cgcgccgcaa gcagcaccgc caacggccgc aggcgcttgc cgccgttgtg aatgatgtgg
     2701 tgcgcgacct ggttcacgag cgcgacgtcg ctgcccaagc gccgcacgat catgcgattg
     2761 acgtcggccc aatcgtcgcc gacgagcgcc cgcaacgcct cgagcgaggg cccttccgga
     2821 ttcggcttca ccaacgacat gcgcaaattt tgcctgaacc ggccccctcg agcgaggcag
     2881 ccgaacgtcg aagaggccgc gtgcctggcg gcgggcgcac ctcgaagggc cctcccggga
     2941 gacgccggaa gaccatccct ggggctcgtg ttcgcacatc catgtgctca cacgctcccg
     3001 ggagggccct tcg
//
LOCUS       DASSNN010000776         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1720346, whole genome shotgun sequence.
ACCESSION   DASSNN010000776 DASSNN010000000
VERSION     DASSNN010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720346"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VFL84_14565"
     CDS             complement(<1..629)
                     /locus_tag="VFL84_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HET7609901.1"
                     /translation="MASERARPRAPPFIDPPGYACHRPEATLLYQLVERHYPELVAAR
                     EGARRPLPKYVQEEFEAYLKCGRLEHGFLRVRCEDCHAEKLVAFSCKRRGFCPSCGAR
                     RMTDSAAFLADEVSPAKPIRQWVLSLPLALRFLLATDPDALSQVLGTVYRTISAHVLK
                     KARLTRASGATGAVTLIQRFGSALNLNVHFHLLVLDGAYLVHTEPPVFRR"
     gene            789..1529
                     /locus_tag="VFL84_14570"
     CDS             789..1529
                     /locus_tag="VFL84_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET7609902.1"
                     /translation="MVAIKRVVDYNVRVRVKPDGSGMATEGLKMSINPFDEIAIEAAL
                     RLKESGTFANVVLVTIGPVEAEQQLRTGLAMGADRAIRVNAPAQLEPLAVARALHALA
                     LREQPGLVVLGKQAVDDDHGQTGQMLAALWDRPQATFASQLSVAGDVATVTREIDAGL
                     ETIEVDLPAVVTTDLRLNQPRYVKLPEILKAKSKPLAVIPLAELGVDAARQFTVHDFA
                     PPPARAAGVRVKDVAELVAALESRGVLP"
     gene            1526..2470
                     /locus_tag="VFL84_14575"
     CDS             1526..2470
                     /locus_tag="VFL84_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7609903.1"
                     /translation="MTRVLVVAEHDGARLDAATAKCVRCALDLKPTALVVAVLADGGA
                     DVAAAAAKLEGVTEVLHVDAPHNAAPLAAILAPQVAALAKEFTHVLGPSSTFGKDLLP
                     RVAALAGVGQVSDVLAIVGPHRFRRPVHAGNAVVTVDADPRRLVVATVRLASYRGIGE
                     GAPAPVEKRTLDVVLPKHTRFVERRASGGERPDLMTAQRVVSGGRALGSAEGFKLVAE
                     LADVLGAALGASRAAVDSGFVANDLQVGQTGKIIAPELYVAVGISGAIQHLTGIKDAR
                     VIVAINKDAEAPIFEVADIGLVADLFEAVPRLVAALKR"
BASE COUNT          388 a          858 c          873 g          370 t
ORIGIN      
        1 cggcggaaca ccggcggctc ggtgtggacg aggtacgccc cgtcgagcac gagcaggtga
       61 aagtgaacat tgaggttgag cgccgaccca aaccgctgga tcagcgtgac ggcgccggtc
      121 gcaccgctcg cgcgcgtgag ccgagccttc ttgagaacat gggccgagat cgtgcggtag
      181 accgtcccga gcacttggct caacgcgtca gggtcggtgg cgagcaagaa acgcagcgcc
      241 aacggcagcg acagcaccca ctggcgaatc ggcttcgccg gtgacacctc gtccgcaaga
      301 aacgcggcac tgtccgtcat tcgccgcgca ccgcaggagg gacaaaagcc gcggcgctta
      361 cagctgaacg cgacgagctt ttcggcgtgg cagtcctcgc agcgcacccg cagaaacccg
      421 tgctctaagc ggccgcattt cagatacgcc tcgaactcct cctgcacgta tttcggcagc
      481 ggtcggcgcg cgccttcgcg cgcggcgacg agctcggggt agtgccgctc gacgagctgg
      541 tagagcagcg tcgcctcggg ccggtggcag gcatacccgg gcgggtcgat gaacggtggc
      601 gcccgcgggc gtgcccgctc gctcgccatg ggctcgcgtc cttgcgatgg agggtggacg
      661 cgaagactct acgcaggtag gccgcgacgg cggcatcgcg aatctcagcg aatcaaatgg
      721 attcgaggcg cgatgctaaa atttgacgac gatttcgcgc ccgcggggta ccttccttgc
      781 aaacgatcat ggtcgccatc aagcgcgtcg tggactacaa cgtccgcgtg cgcgtgaagc
      841 ccgacggctc cggcatggcg accgaagggc tcaagatgag catcaatccc ttcgacgaga
      901 tcgcgatcga ggcggcgctt cgcctcaagg agtcgggcac gttcgcgaac gtcgtgctcg
      961 tcacgatcgg ccccgtcgaa gccgagcagc agttgcggac cggcttggcg atgggcgccg
     1021 atcgcgcgat tcgcgtcaac gcgcccgcgc agctcgagcc cctcgccgtc gcccgcgccc
     1081 tgcacgcgct cgctctgcgc gagcagcccg gcctcgtcgt gctcggcaag caagccgtcg
     1141 acgacgacca cgggcaaacg gggcagatgc tggccgcgct gtgggaccgg ccgcaggcga
     1201 cgttcgcgtc gcagctttcg gtcgcgggcg acgtcgcgac cgtgacgcgc gagatcgacg
     1261 cggggctcga gacgatcgag gtcgacttgc ccgccgtcgt cacgacggac ttgcggctga
     1321 atcagccccg ctacgtcaag ttgcccgaaa tcttgaaagc gaagtcgaaa ccgctcgccg
     1381 tgattccgct cgcggagctc ggcgtcgacg cggcgcggca attcaccgtg cacgatttcg
     1441 cgccgccgcc ggcacgcgcg gccggcgttc gcgtcaaaga cgtggccgag ctcgtcgccg
     1501 cgctcgagag ccgcggggtg ctgccgtgac gcgcgtgctc gtcgtcgccg agcacgacgg
     1561 cgcgcggctc gacgccgcga cggcgaagtg cgtgcgctgc gcgctcgatc tgaagcccac
     1621 ggcgctcgtc gtcgccgtgc tcgccgacgg cggcgccgac gtcgcggcgg cggccgcgaa
     1681 gctcgagggc gtcaccgagg tcttgcacgt cgacgcgccg cacaacgcgg cgccgctcgc
     1741 ggcgattctc gcgccgcagg tcgcggcctt ggccaaagag ttcacacacg tgctcggccc
     1801 ttcttcgacg ttcggcaagg atttgctgcc gcgcgtcgcg gcgctcgccg gcgtcggcca
     1861 agtcagcgac gtgctcgcga tcgtgggccc gcatcgcttt cgccggcccg ttcacgccgg
     1921 caacgccgtc gtcacggtcg acgcggatcc gcggcggctc gtcgtcgcga cggtccggct
     1981 cgcgtcgtac cgcggcatcg gcgaaggcgc gcccgcgccc gtcgagaagc gcacgctcga
     2041 cgtcgttctg ccgaagcaca cgcgcttcgt cgagcggcgc gcgagcggcg gcgagcggcc
     2101 ggacttgatg accgcgcagc gcgtcgtgtc gggcggccgc gcgctcggca gcgccgaggg
     2161 gttcaagctc gtcgccgagc tcgccgacgt gctcggcgcg gcgctcggcg cgtcgcgcgc
     2221 ggccgtcgac tccggtttcg tcgcgaacga tctgcaagtc ggccagaccg gcaagatcat
     2281 cgcgcccgag ctctacgtcg ccgtcggcat ctcgggcgcg atacaacatc tgaccggcat
     2341 caaggatgcg cgcgtcatcg tggcgatcaa caaggatgcc gaggcgccga tcttcgaggt
     2401 cgccgacatc ggcctcgtgg ctgacttgtt cgaggccgtg ccgcggctcg tcgcggcctt
     2461 gaagagatga aagcggcctt gaagagatg
//
LOCUS       DASSNN010000777         3856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_706178, whole genome shotgun sequence.
ACCESSION   DASSNN010000777 DASSNN010000000
VERSION     DASSNN010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706178"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VFL84_14580"
     CDS             <1..1005
                     /locus_tag="VFL84_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET7609904.1"
                     /translation="VGAYVYYTSGRIVGTDNAYVKADVGVISAEVAGPIATLNVRENQ
                     RVEAGDVLFTIDDRPFRVALDRGNAQLAAINDFVESTKASYRQSLEQLALARTNAAYE
                     QRELDRLTALAERKLTSDVAVDEQRHERDVANQEIQVTERALDAIRARLGGDLDRPVT
                     EQAAYLAAKSMRDAAELDLEHTVVRAPFSGIASQVPTLGHHVQPGSPIMTIVADRDMW
                     IEANFKETDLTHVAVGQPVDIRLDTYPDRRWRGSVASISQATGAEFSVIPAQNATGNW
                     VKVAQRIPVRIAIEMRGDDPELRAGMSAIVDIDTGHERPAPHWIRALLPSNSAFAESR
                     "
     gene            1012..2541
                     /locus_tag="VFL84_14585"
     CDS             1012..2541
                     /locus_tag="VFL84_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007636823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET7609905.1"
                     /translation="MSATAINATASAAAPLNRGMITLSVMLATVMQVVDTTIVNVALP
                     HMQGSMAATQDQISWVLTSYIVAAAIMMPLTGVLADRFGRKRLFATAVIGFTLASMLC
                     GAATSLEQMVVFRALQGALGASLVPLSQAVLLDTYPREKHGSAMALWGVGVMVGPILG
                     PTLGGYLTEYYTWRWAFYINLPVGILAVLGIAAFVPEAKGGESRGFDYFGFALLSIAI
                     GSLQLMLDRGNSLDWFDSTEVVLEAATAALAFYLFLTHMFTADKPFLEPALFADRNFV
                     AGVLLMFTVGVMLMSTMALLPPFLQNLLGFPVLTAGYVLAPRGAGTMLAMMVVGRLVG
                     KVDTRLLVLAGLGLMAWSLREMTQWTAEVGVSEIISTGIVQGIGLGFIFVPLSTIAFS
                     TLAPRYRNEGTAMFSLVRNIGASVGISIVMTVLGHEVQKSHAELSTSITPFRDAFQAP
                     GVPPLWDLSTELGRIALDAEVSRQAVTIAYLNDFRFMMYLCLLTLPLLLLLRPRRSAP
                     R"
     gene            2634..3389
                     /locus_tag="VFL84_14590"
     CDS             2634..3389
                     /locus_tag="VFL84_14590"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7609906.1"
                     /translation="MKRYYVCLSFAVGVLVVAAANAQSAAKATVADRVVIGSPYAIKA
                     DAEADAFVTATAEKAYAQNCASCHGADMHGKAGVPDLTDFEFIWGITGEETSDVGPVA
                     ELEQTIRYGVRNQDCPAVHDQALYGDCPDTRYSEMPAFATLGSLTGEQIRDMTEYMLS
                     LSGGQADAAAAARGKMEFGQCVECHGPEGMGYKPYGGPDLTDDIWIYGGDRTAIAASI
                     SAGRMGRCPAWVGKLDAATIKSLAVYIWRKVQG"
     gene            3483..>3856
                     /locus_tag="VFL84_14595"
     CDS             3483..>3856
                     /locus_tag="VFL84_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609907.1"
                     /translation="MKLMLAAGLLAAFGSATVVAQPAPTPFKLGTFERQGRPFVGIVL
                     RENVVIDFAAANAAVTSPASNVAAPADMKDLIARYDSGLRARIVEIVRAVGDTRPAYV
                     YDLSALKVLPPVMPTTMLNVAV"
BASE COUNT          617 a         1322 c         1277 g          640 t
ORIGIN      
        1 gtcggcgcct acgtctatta cacgtcggga cgaatcgtcg gaaccgacaa cgcgtacgtc
       61 aaagccgacg tcggcgtcat cagcgcggag gtcgcggggc cgatcgccac gctgaacgtg
      121 cgcgagaacc agcgcgtcga agccggcgac gtgctgttca cgatcgacga ccgccccttc
      181 cgcgtcgcgc tcgatcgcgg caatgcgcag ctcgcggcga tcaacgactt cgtcgagagc
      241 accaaggcgt cgtaccggca atcgctcgag cagctcgcgc tcgctcgcac gaacgcggcc
      301 tacgagcagc gcgagctcga ccggctcacc gcgcttgcgg agcgcaagct cacgtccgat
      361 gtggccgtcg acgagcaacg ccatgaacgc gacgtcgcca accaagagat tcaggtcacg
      421 gagcgcgcgc tcgatgccat ccgcgcgcgt ctcggcggcg atctcgaccg gcccgtgacc
      481 gagcaagccg cgtatctcgc cgcaaaatcg atgcgcgacg ccgccgagct cgatctcgag
      541 cacacggtcg tgcgcgcgcc gttcagcggc atcgcgagcc aagtgccgac gctcggccat
      601 cacgtccagc ccggatcgcc gatcatgacg atcgtcgccg atcgcgacat gtggatcgag
      661 gcgaacttca aagagaccga cttgacgcac gtcgccgtcg gccagcccgt cgacatacgc
      721 ctcgacacct accccgatcg ccggtggcgc ggcagcgtcg cgagcatcag ccaggcgacg
      781 ggcgccgagt tctcggtgat tccggcgcag aacgcgaccg gcaattgggt caaggtcgcg
      841 caacggattc ccgtgcgcat cgcaatcgag atgcgcggcg acgatccgga gttgcgcgcc
      901 ggcatgagcg cgatcgtcga catcgacaca ggtcacgagc ggcccgcgcc gcattggatc
      961 cgcgcactgc tgcccagcaa ctccgcgttc gcagagagcc gctagccggt catgagcgcg
     1021 acggcgatca acgcgacggc gagcgctgca gcgccgctga accgcggcat gatcacgttg
     1081 tcggtcatgc tcgcgacggt catgcaggtc gtcgacacga cgatcgtcaa cgtcgcattg
     1141 ccgcacatgc aaggcagcat ggccgcgact caggaccaga tttcctgggt gctgacgtcc
     1201 tacatcgtcg ccgcggcgat catgatgccg ctgaccggcg tgctcgccga ccgcttcggg
     1261 cgcaagcggc tgttcgccac ggccgtcatc ggcttcacgc tggcctcgat gctgtgcggc
     1321 gcggccacgt cgctcgagca aatggtcgtg tttcgtgcgc tgcaaggcgc gctcggcgcg
     1381 agcctcgtgc cgctctcgca agcggtgctg ctcgacacgt atccgcgcga gaagcacggc
     1441 tcggcgatgg cgctatgggg cgtcggcgtc atggtcggcc cgatcttggg gccgacgctc
     1501 ggcggctacc tgaccgagta ctacacctgg cggtgggcgt tctacatcaa cctgcccgtc
     1561 ggcatcttgg ccgtgctcgg catcgcggcc ttcgtgcccg aggccaaggg cggagagtcg
     1621 cgcggcttcg actacttcgg cttcgcgctg ctcagcatcg cgatcggctc gctgcagctc
     1681 atgctcgacc gcggcaactc gctcgactgg ttcgactcga ccgaggtcgt gctcgaagcg
     1741 gcgacggcgg cgctcgcctt ctatctgttc ctgacccaca tgttcacggc cgacaagccg
     1801 ttcctcgagc ccgccctgtt cgcggaccgc aacttcgtcg ccggcgtcct gctgatgttc
     1861 acggtcggcg tgatgctgat gtcgacgatg gcgctgctgc cgccgttcct gcagaacctg
     1921 ctcggctttc ccgtgctgac ggcgggctac gtgctcgcgc cgcgcggcgc gggcacgatg
     1981 ctcgcgatga tggtcgtcgg caggctcgtc ggcaaggtcg acacgcgcct gctcgtgctc
     2041 gccgggctcg gcctcatggc gtggtccttg cgcgagatga cgcagtggac tgcggaggtc
     2101 ggagtctcgg agatcatctc gacgggcatc gttcagggca tcggcctggg cttcatcttc
     2161 gtcccgctgt ccacgatcgc gttctcgacg ctggcaccgc gctaccgcaa cgaagggacc
     2221 gcgatgttca gcctggtccg caacatcggc gccagcgtcg gcatctcgat cgtcatgacc
     2281 gtgctcggcc acgaggtgca gaagagccac gcggagttga gcacgagcat cacgccgttt
     2341 cgcgacgcgt ttcaggcgcc cggcgttccg ccgctgtggg acctttcgac cgagctcggg
     2401 cgcatcgcgc tggacgcgga agtgtcgcgg caagcggtca cgatcgctta tctgaacgac
     2461 ttccgcttca tgatgtacct ctgcttgctc acgttaccgt tgctcttgct gctgcgtccg
     2521 cgccggtccg cgccgcgtta accgtgcata accgttatta actgccgcgc ggcgctcgcg
     2581 cggcgttaga gtccgcgctc ggtgaaggcc caccgcacgc ccggaggctc tgcatgaagc
     2641 gctattacgt ttgcctctca tttgcagtcg gagtcctcgt ggtcgcggcc gcgaacgcgc
     2701 agagcgcggc gaaagcgacc gtcgcggatc gcgtcgtcat tgggtcgccg tacgcgatca
     2761 aggccgacgc cgaagccgac gcgttcgtga cggcgacggc cgagaaggcc tacgcgcaga
     2821 actgcgcgag ctgccacggt gccgacatgc acggcaaggc cggcgtgccg gatctcaccg
     2881 acttcgagtt catctggggc atcacgggcg aggagacgag cgacgtcggc ccggtcgccg
     2941 agctcgagca gacgatccgc tacggcgtgc gcaatcagga ctgtcccgcc gtgcacgacc
     3001 aggcgctcta cggcgactgc cccgacacgc gctactccga gatgccggct ttcgcgacgc
     3061 tcggctcgct gacgggcgag caaatccgcg acatgaccga gtacatgctg tcgctgtcgg
     3121 gcgggcaagc cgatgcggcg gccgccgcgc gcggcaagat ggagttcggc cagtgcgtcg
     3181 agtgccacgg gccggagggc atgggataca agccgtatgg cgggcccgac ttgaccgacg
     3241 acatctggat ctatggcggc gaccgcaccg cgatcgcggc ctcgatctcg gcgggccgca
     3301 tgggccgctg ccccgcgtgg gtcggaaagc tcgacgccgc gacgatcaag tcgctcgccg
     3361 tgtacatctg gcgcaaggtc cagggctaaa ccgcgccgcg ttggcggcgg aagcgccgcg
     3421 cacgttacga tgacctcgag acccgcggcg ccgtgccgcg gacgcactgg ggggaatcgc
     3481 tcatgaagtt gatgcttgca gcagggctcc ttgcggcctt tggctcagcg actgtcgtcg
     3541 cacaacccgc tccgacaccg ttcaagctcg gcacgttcga gcgccagggc cggccgttcg
     3601 tcggcatcgt gctgcgcgaa aacgtcgtca tcgatttcgc ggccgcgaac gcggccgtca
     3661 cgagcccggc gtcgaacgtt gccgcgccgg ctgacatgaa agatctcatc gcgcgttacg
     3721 acagcggctt gcgcgcgcgc atcgtcgaga tcgtacgcgc cgtcggcgat acgcgtcccg
     3781 cctacgtcta tgacttgagc gcgctgaaag tgctgccccc ggtcatgccg acgacgatgc
     3841 tgaacgtcgc cgtcaa
//
LOCUS       DASSNN010000778         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1885601, whole genome shotgun sequence.
ACCESSION   DASSNN010000778 DASSNN010000000
VERSION     DASSNN010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885601"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1824
                     /locus_tag="VFL84_14600"
     CDS             <1..1824
                     /locus_tag="VFL84_14600"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7609908.1"
                     /translation="ILVTTVEPGDEFGGTFRGGFGSDSLLDAFAAFDAPFSDNVKSRF
                     SIGTRQQDGYVTRPDGTDLGDTDTWTASAKLLVAPSDTFSATFALDYTKSDENGSPLV
                     FAAMTETATFPRVASLDAGCPGLTDGAVPPPGLPDQPAVPMIQDDRCANDLQARGPYA
                     NNGTLPLESKLESSGLSATLAWDFSDTLSLESITSFRNLDWGGVRDADNTPLPILHTI
                     YDVDSSQWSQELQLTLQRDALTGVVGFYYFAQESDDIVTVELNPPPPGIQRDSDNNAV
                     DNDSWAVFTQWTYDFTEQWSVTGGGRYTEETKRAFPDQFDFANPAAKQVPAQWYEDTF
                     SDFTPSASLAFRWSPQGMLYLSYAQGFKGGGWNSHFNAPLTPEQQAALHPFRPEEAES
                     IELGTKLDLAANKLRLNAALFSSDYDDMQLTYRGPAPTGVAPFVTNAGKTSIDGAELE
                     LTWIATSRLLIDASVGTLDATIDELAAIPFAVLPPDLLPGNALPFSPDHQAHLGLAYD
                     AGRGNVTITPRVDVSYTDTQFFDATNTREIAQLEDVTVVNASVFIQPSAANWRLTIGV
                     NNATDELYPIAGNSSLSTGSGYAEIAYARPREYFATINFDF"
     gene            1844..>2370
                     /locus_tag="VFL84_14605"
     CDS             1844..>2370
                     /locus_tag="VFL84_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7609909.1"
                     /translation="MRTQVAIIGAGPAGLLLGQLLHKAGIDAVVLERQTADYVLGRIR
                     AGVLEQVSVDLLDEAGVGERMHREGLVHSGFELLAGGARHRVDLEALSGGKHVVVYGQ
                     TELTRDLMEARAAAALPMVYEARDVAVHGFDRASPHVTYRKDGREQRLDCDFIAGCDG
                     YHGVCRASVPKSAIAT"
BASE COUNT          409 a          843 c          755 g          363 t
ORIGIN      
        1 atcctcgtga cgaccgtcga gccgggcgac gaattcggcg gcacgtttcg cggcggcttc
       61 ggcagcgaca gccttttgga cgcgttcgcg gcgttcgacg cgccgttctc ggacaacgtg
      121 aagtcgcgct tctcgatcgg aacgcggcag caggacggct acgtcacgcg ccccgacggt
      181 accgatcttg gcgacaccga cacctggacg gcgagcgcga agctgctcgt cgcgccgagc
      241 gacacgttca gcgcgacgtt cgcgctcgac tacacgaaat ccgacgagaa cggctcgccg
      301 ctcgtgttcg cggcgatgac cgaaacggcc acgtttccgc gcgtcgcgag cctcgacgcc
      361 ggctgcccgg gcctcacgga cggcgccgtc cctccgcccg gacttccgga tcagccggcc
      421 gtgccgatga ttcaagacga ccgctgtgcg aacgatcttc aggcgcgcgg gccgtacgcc
      481 aacaacggca cgcttccgct cgaaagcaag ctcgagagct cgggcctgtc cgcgacgctg
      541 gcttgggact tcagcgacac gctctcgctc gagtcgatca cgtccttccg caatctcgat
      601 tgggggggcg tccgcgacgc cgacaacacg ccgctcccca tcctgcatac gatctacgac
      661 gtcgactcgt cgcaatggag ccaggagctg caactgacgc tgcagcgcga cgcgctgaca
      721 ggcgtcgtcg gtttctacta cttcgcgcag gagtccgacg acatcgtgac ggtcgagctc
      781 aatccgccgc cgcccggcat ccagcgcgac agcgacaaca acgccgtcga caacgacagc
      841 tgggccgtgt tcacgcaatg gacgtacgac ttcaccgagc agtggagcgt gacgggcggc
      901 ggccgctaca cggaagagac gaagcgcgcg tttcccgatc agttcgattt cgcgaacccc
      961 gcagcgaagc aagtgcccgc gcagtggtac gaggacacgt tcagcgattt cacgccgtct
     1021 gcctcgctcg cgttccgctg gtcgccgcaa ggcatgctgt acctgagcta cgcgcagggc
     1081 ttcaaaggcg gcggctggaa cagccacttc aacgcgccgc tgacgcctga gcagcaggcg
     1141 gcgctccatc cgttccgccc cgaggaggcc gaatcgattg agctcggcac gaagctcgac
     1201 ctcgccgcca acaagctgcg cttgaacgcg gcgcttttca gcagcgacta cgacgacatg
     1261 cagctcacgt atcgcggccc tgctccgacc ggagtcgcgc cgttcgtcac gaacgccggc
     1321 aagacgagca tcgacggcgc cgagctcgag ctgacgtgga tcgcgacctc gcgtttgctg
     1381 atcgacgcga gcgtcgggac gttggacgcc acgatcgacg agctcgccgc cattccgttc
     1441 gccgtattgc ccccggactt gcttcccggc aacgcgctgc cgttctcgcc ggaccaccaa
     1501 gcccacctgg gtctcgccta cgacgccggc cgcggaaacg tcacgatcac gccgcgcgtc
     1561 gacgtgtcgt acaccgatac gcagttcttc gacgcgacga acacgcgcga gatcgcgcag
     1621 ctcgaagacg taaccgtcgt caacgcgtcg gtcttcattc agccttccgc ggccaattgg
     1681 cgcctcacga tcggcgtgaa caacgcgacc gacgagcttt acccgatcgc cggcaactcg
     1741 tcgttgtcga ccggctcggg ctacgccgaa atcgcctacg cccggccgcg cgagtacttc
     1801 gcgacgatca acttcgactt ctgagggcgg cgccgcgacg gacatgcgca cgcaggtggc
     1861 aatcatcggc gcgggccccg cgggcctgct gctcgggcag ctcttgcaca aagccggcat
     1921 cgacgccgtc gtgctcgagc ggcaaaccgc ggactacgtg ctcggccgca tccgggccgg
     1981 cgtgctcgag caagtcagcg tcgacctcct cgacgaagcc ggcgtcggcg agcgcatgca
     2041 tcgcgagggg ctcgtgcatt cgggcttcga gctgctcgcc ggcggcgcgc ggcatcgcgt
     2101 cgatctcgag gcgttgagcg gcggcaagca cgtcgtcgtc tacggccaga ccgagctcac
     2161 gcgcgacttg atggaagcgc gcgccgccgc cgcgctgccg atggtctatg aagcgcgcga
     2221 cgtcgccgtg cacggcttcg accgcgcaag cccgcatgtg acgtaccgca aagacggccg
     2281 cgagcagcgc ctcgactgcg acttcatcgc gggctgcgac ggctaccacg gcgtctgccg
     2341 cgcgagcgtg ccgaagagcg cgatcgcgac
//
LOCUS       DASSNN010000779         4408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1720562, whole genome shotgun sequence.
ACCESSION   DASSNN010000779 DASSNN010000000
VERSION     DASSNN010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720562"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFL84_14610"
     CDS             <1..459
                     /locus_tag="VFL84_14610"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET7609910.1"
                     /translation="AELTPLIENTNPVVLVVMLGGAFTAIELCKRFRFAHELDYVHVT
                     RYRGTTRGGAVSWRVRPRRALKGRTVLVVDDILDHGKTLRALDDELERIEVERRLCAV
                     LVVKRIAAADRPSVTVAGLAVDDVYVFGSGMDYRGYWRGLAGIYAVEERA"
     gene            456..1193
                     /locus_tag="VFL84_14615"
     CDS             456..1193
                     /locus_tag="VFL84_14615"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HET7609911.1"
                     /translation="MTPFALIVGSGFERLGFDVLARSPTKTPYGEPSSAVLTLALRGA
                     RVPCIARHGEQHHLAPHEINYRANLWALEQRGVRACIGINTVGAIDASLRPGELAVPD
                     QLIDYTYGRVSTFRAADEPVRHIEFDEPFSASLRERIAAAASACGFAVRAGTYGVTQG
                     PRLETAAEIERLARDGCGMVGMTAMPEAALARELGIDYAICAVAVNWAAGRSPDGAPI
                     FEQIAGFMDAGWRKIGAVLERLVGDAV"
     gene            1239..2252
                     /locus_tag="VFL84_14620"
     CDS             1239..2252
                     /locus_tag="VFL84_14620"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7609912.1"
                     /translation="METGRDAFGIRSLNRHTLEQIVSASSAGILVVDASHPELPIVYA
                     NAAYERLTGYALSELAGHPWAPLARAEDGDAVLAPLKTAIGRGAACRASIPDVRKDGT
                     SFSCDISVTPLHGPRGDLRYFLLSHEHATSIEQPASAPQPSFAEEQPPADAASAEIAL
                     LQRELGRARQKIATIDRIDQGTGLVRFAYFQETLRRDLAMARRDRRFVTILVFEIVEF
                     AVYRQTFGDKAADSCQRMIGAQIMRALRRAGDLCARYDDTTLVAAVVGQHASEVQPLA
                     ERIAESVGQLKLHNPRAKGSRYIETRAIVIGCPPGAHDDPGPLIERAIAEARGNSLAA
                     RVG"
     gene            complement(2254..3000)
                     /locus_tag="VFL84_14625"
     CDS             complement(2254..3000)
                     /locus_tag="VFL84_14625"
                     /inference="COORDINATES: protein motif:HMM:NF022425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HET7609913.1"
                     /translation="MRRILASVAASVACAAAAMAQAPPAADPEPIPRYEVEVIVFAHR
                     QFDPTEEHFDQTLNGFGDGAAALREAPVFDPSTFAPSGAPAPVFEPLAEVDPAAAERA
                     DALSVRPLRSEELKLVNEYRKIRAVSAYEPLAHFGWVQPGLPETDAVPVDLGSLGILN
                     PSGTVRVHLTRYLHVTLDLTYQASPSTTPAESSVDGLDEIVLAPQYRLTTTRRVISNE
                     LHYFDHPAFGVLVRVTPVQEKEAGGRRPAA"
     gene            complement(3031..>4408)
                     /locus_tag="VFL84_14630"
     CDS             complement(3031..>4408)
                     /locus_tag="VFL84_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HET7609914.1"
                     /translation="VLQNLESGQTDIVIGTHRLLQSGVKFGRLGLLIIDEEHRFGVKD
                     KERLKRLRAEVDMLTLTATPIPRTLNMTLGGLRDLSVIATPPAERLPVKTFVGEWSDR
                     QIREAVLREIRRGGQIYFVHNRVENIESVAEDLRKLLPEAEIRVGHGQMSERELELVM
                     LDFYHREFNVLVCTTIIESGIDIPTANTIIINRADRLGLAQLHQLRGRVGRSHHQAYA
                     YLLAPPRSSLTPDAIKRLEAIESLEELGSGFTLATHDLEIRGAGELLGEGQSGQIQAI
                     GFTLYNELLGRAVKALRSGQEPDLEDPFTHGPEIEIGLAALIPEDYMPDVHMRLVHYK
                     RIANCRSGLELDSLQIELIDRFGLLPPPLKTLFAITALKLLAEQIGIAKIQAGAESGS
                     ITFAERSRVDPARLLKLIESDSKRYRLDGPYKLRFSWTLENPERRVSALDKLLRRLGA
                     AEATAAAA"
BASE COUNT          673 a         1473 c         1542 g          720 t
ORIGIN      
        1 gccgagctga cgccgttgat cgagaacacg aatcccgtcg tgctcgtcgt catgctcggc
       61 ggcgcgttca cggcgatcga gctctgcaag cgcttccgct tcgcgcacga gctcgactac
      121 gtccacgtca cgcgctaccg cggcacgacg cgcggcggcg cggtctcttg gcgcgttcgg
      181 ccgaggaggg cgctgaaagg gcgaacggtg ctcgtcgtcg acgacatcct cgatcacggc
      241 aagacgctgc gcgcgctcga cgacgagctc gagcgtatcg aagtcgagcg gcggctctgc
      301 gccgtgctcg tggtgaagcg catcgcggct gccgatcggc cgagcgtcac ggtcgccggt
      361 ctcgccgtcg acgacgttta cgtgttcggc agcggcatgg actaccgcgg ctactggcgc
      421 gggcttgccg gcatctacgc cgtcgaggag cgcgcatgac gccgttcgcg ctgatcgtcg
      481 gcagcgggtt cgagcgcctc ggtttcgacg tgctcgcgcg aagcccgacg aagacgccgt
      541 acggcgagcc gagcagcgcc gtgctgacgc tcgcgctccg cggtgcgcgc gtgccctgca
      601 tcgctcggca cggcgagcag catcatctcg cgccgcacga gatcaactac cgagcgaatc
      661 tctgggcgct cgagcagcgc ggcgtgcgcg cgtgtatcgg gatcaatacc gtcggcgcga
      721 tcgacgcgtc gcttcgcccg ggcgagctcg ccgtgccgga tcagctcatc gactacacgt
      781 atgggcgcgt ctcgacgttc cgcgccgcgg acgagccggt gcgccacatc gagttcgacg
      841 agccgttctc ggcgagcttg cgcgagcgga tcgccgcggc ggcgtcggcc tgcggtttcg
      901 cggttcgagc cggcacttac ggcgtcacgc aggggccccg cctcgagacg gcggccgaga
      961 tcgagcgcct cgcgcgcgac ggctgcggga tggtgggcat gacggcgatg cccgaggcgg
     1021 cgctggcccg ggagctcgga atcgactacg cgatttgcgc cgtggcggtc aattgggcgg
     1081 ccgggcgttc acccgatggg gcgccgatct tcgagcagat cgcgggcttc atggatgccg
     1141 gctggcgaaa aataggcgcg gtcctcgaac ggctcgtcgg cgacgcagtg tgatgcgttt
     1201 catagacgct cgttgcgtgc gtcacagaac ataatcgcat ggaaaccggc agggacgcat
     1261 tcggcatcag atccctgaac cgccacacct tggagcagat cgtctccgca agctcggcgg
     1321 gcattctggt tgtcgacgcg agccatcccg agctgccgat cgtctacgcg aatgcggcgt
     1381 acgagcggtt gaccggctac gcgctcagcg agctcgcggg gcatccgtgg gcgccgctcg
     1441 cgcgcgccga ggacggcgac gcggtgctcg cgccgctgaa gaccgcgatt ggccgcggcg
     1501 ccgcctgccg cgcctcgatt cccgacgtcc gcaaagacgg cacgtcgttc agttgcgaca
     1561 tcagcgtcac gccgctgcac ggccctcgcg gcgacctgcg ctatttcttg ttgagtcatg
     1621 agcacgcgac gtcgatcgag cagccggcgt ccgcgcctca gccctctttc gcggaggagc
     1681 agccgcccgc ggacgctgcg agcgccgaga tcgcgttgct gcagcgcgag ctcggccgcg
     1741 cgcggcagaa gatcgcgacg atcgatcgga tcgatcaggg caccgggctc gtgcgcttcg
     1801 cgtatttcca ggagacgttg cggcgcgatc tcgcgatggc gcggcgcgac cggcgtttcg
     1861 tcacgatact cgtgttcgag atcgtcgagt tcgccgtcta tcggcaaacg ttcggcgaca
     1921 aggccgcgga ctcctgccag cgcatgatcg gcgcgcagat catgcgcgcg ctgcgccgcg
     1981 ccggagacct gtgcgctcga tacgacgaca cgacgctcgt cgcggcggtt gtgggtcagc
     2041 acgcgagcga ggtccagcca ttggcggagc gcatcgccga aagcgtgggg cagctcaagc
     2101 tgcacaatcc gcgcgcgaag ggcagccggt acatcgagac acgcgcgatc gtgatcgggt
     2161 gcccgccggg ggcccacgac gatcccggcc cgttgatcga gcgcgcgatc gccgaggcgc
     2221 gcggcaacag tctcgccgcc cgcgtcggct agcctaagcc gccggccgcc ggccgcccgc
     2281 ttccttctcc tgcacgggcg tcacacgcac gagcacgccg aaggcgggat gatcgaagta
     2341 gtgcagctcg ttgctgatca ctctgcgcgt ggtcgtcagc cgatactgcg gcgcaaggac
     2401 gatctcgtcg agcccatcga cgctgctctc tgccggcgtc gtgctcggcg acgcttggta
     2461 ggtcaggtcg agcgtaacgt gcaagtagcg tgtcaggtgc acgcgcaccg tgccgctcgg
     2521 attcaagatg ccgagcgacc cgagatcgac cggcaccgcg tcggtttcgg gcaggccggg
     2581 ctgcacccag ccgaagtgcg cgagcggctc gtaggccgag accgcgcgaa tcttgcggta
     2641 ctcgttgacg agcttcagct cctcggaacg caacggccgc acgctcagcg cgtcggctcg
     2701 ctcggccgcg gccgggtcga cctcggcgag cggctcgaag acgggcgccg gtgcgccact
     2761 cggcgcaaac gtggacgggt cgaagacggg cgcctcgcgc agtgccgcgg caccgtcgcc
     2821 gaaaccgttc agggtctggt cgaaatgctc ttcggtcggg tcgaactgcc ggtgcgcgaa
     2881 cacgatgacc tcgacctcgt agcgcggaat cggctccggg tcggccgcgg gcggcgcctg
     2941 tgccatggcg gcggccgcgc acgcgaccga tgctgcaacg gaggcgagaa tccttctcat
     3001 cggggcggag tatagcgcgt tgccgcggcg tcaggccgcg gcagccgtcg cttcggccgc
     3061 gcccaagcgc cgaagcagct tgtcgagcgc cgaaacgcgc ctttccgggt tctcgagcgt
     3121 ccacgagaac ctgagcttgt aaggcccgtc gagccggtag cgcttcgagt cgctctcgat
     3181 gagcttgagc agccgggccg ggtccacgcg cgagcgttct gcgaacgtga tgctgccgct
     3241 ttcggcgccg gcttgaattt tcgcgatgcc gatttgctcg gcgagcagct tcaacgccgt
     3301 gatcgcgaac agtgtcttca gcggcggcgg cagtagcccg aatcggtcga tgagctcgat
     3361 ctgcagggag tcgagctcga ggcccgaccg gcagttcgcg atgcgcttgt agtgcacgag
     3421 ccgcatgtgc acgtccggca tgtagtcctc ggggatcagc gcggcgagcc cgatctcgat
     3481 ctcggggccg tgcgtgaacg ggtcctcgag atcgggctcc tgacccgagc gaagcgcctt
     3541 gacggcgcgg ccgagcagct cgttgtagag cgtgaagccg atcgcctgaa tttggccgct
     3601 ctggccctcg ccgagcagct cgccggcgcc gcgaatctcg agatcgtgcg tcgcgagcgt
     3661 gaagcccgag ccgagctcct cgagcgactc gatcgcctcg aggcgcttga tcgcgtcggg
     3721 cgtcaaggag ctccgcggcg gcgcgagcaa gtacgcatac gcctggtggt gcgagcggcc
     3781 gacgcggccg cgcagctgat gcagctgcgc caagcccaag cgatcggcgc gattgatgat
     3841 gatggtgttc gccgtcggaa tgtcgatgcc gctctcgatg atcgtcgtgc agacgagcac
     3901 gttgaactcg cggtggtaga aatcgagcat gacgagctcg agctcgcgct ccgacatctg
     3961 cccgtgtccg acccggattt ccgcctcggg caagagcttg cgcagatcct cggcaaccga
     4021 ctcgatattc tcgacgcggt tgtgcacgaa atagatctgg ccgccgcggc ggatctcgcg
     4081 cagcaccgct tctctgatct gccggtcgct ccattcgccg acgaacgtct tgacgggcag
     4141 ccgctcggcc ggcggggtcg cgatgaccga cagatcgcgc aagccgccga gtgtcatgtt
     4201 cagcgtgcgc ggaatcggcg tcgccgtcag cgtcagcatg tcgacctcgg cgcgcaaccg
     4261 cttcaagcgc tccttgtcct tgacgccgaa gcggtgctcc tcgtcgatga tcagcaggcc
     4321 gaggcggccg aacttcacgc cgctctggag caggcggtgc gtgccgatca cgatgtccgt
     4381 ctgcccggat tcgaggttct gcagcacc
//
LOCUS       DASSNN010000780         3278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_706948, whole genome shotgun sequence.
ACCESSION   DASSNN010000780 DASSNN010000000
VERSION     DASSNN010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706948"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VFL84_14635"
     CDS             complement(<1..833)
                     /locus_tag="VFL84_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET7609915.1"
                     /translation="MFIAGVGQTAVNKDPQSRGRYLGAAAVKEALADAGIEPKTVGAL
                     YVGNMLSGILAQQQQLGGLIADYTGLAGIEAVTIEAACASGGAAVRMAYQAVAGGIHD
                     AVAVCGVERMTHVERDVATRALATAADWELEGVCGESFLSLNATLMRSYMAKHGVDAE
                     RFAPFAITAHRNALTNPNALLKKALDLPTYLESRVVSDPIRLFDVSPVCNGASAVILA
                     APHVARELPNRDSVRIAGSAVATAPLALARRSDPLELTAVSTSTQQAMKQAGVVHADI
                     DL"
     gene            980..1783
                     /gene="proC"
                     /locus_tag="VFL84_14640"
     CDS             980..1783
                     /gene="proC"
                     /locus_tag="VFL84_14640"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET7609916.1"
                     /translation="MSANERIVVVGFGNMGQALVRGWLAKGRGASGIRVVDPAASARA
                     AARELEIAVADTVGTADVVVLAVKPNQLADVLPHCRAAVRPACVFLSIAAGKTLAEIG
                     RGLGQDAAIVRAMPNTPAAIGRGITVLVANAAVSRAQRALCGDLLAAVGSVAWLDDER
                     HMDAVTAISGSGPAYVFLFIECLEQAAIELGLDAALAKHLALATVAGSGAYAESAREP
                     PAELRRRVTSPNGTTQAALDVLMAEPGQRELLARATRAAAERSRELAKV"
     gene            1787..2239
                     /locus_tag="VFL84_14645"
     CDS             1787..2239
                     /locus_tag="VFL84_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET7609917.1"
                     /translation="MTGYYYDEFEVGRVFKHAIRRTMTETDNVLFSTMTHNPAPLHID
                     HEYMRTQSEFGKPLMNSFFTLGLIVGISINDTTLGTTVANLGLEDVRFPAPLFVGDTV
                     HVETEVVAMRPSASRPRNGILTLEHRGYNQNDVLVAACRRNVLMLRSP"
     gene            complement(2241..>3278)
                     /locus_tag="VFL84_14650"
     CDS             complement(2241..>3278)
                     /locus_tag="VFL84_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609918.1"
                     /translation="MTAAFGTLSRLDHRFTAEIVHAGDSVSGERVDAIHGNIVSRSGN
                     RLTVKGAFAVDREHVIRVHRTVLVDVGAGTKVLKTGTPGAVLDPSALSVGQQIVAFGK
                     LTQPASDTMPALLDATNGRVRMQPTHLHGAVKTVVPGQLNLNLRGIDRLGIDAFDFAG
                     TGVTPAQDADPADYEVATGTLGLAGVAAGEPASVIGFVRPFGGAPPDFEGRAVIDHRD
                     LPAMLGIGWGVSGTTAPFSSMGAAGLVLDIHNPSIGARHHLVDGMRVVDLTTLPMAPT
                     LAPADGRAIYGIAVGADIKMFTSFAEFSTELASRLGGGRPAIALTAVGRYEAGSATLH
                     ANHIAVLFAPN"
BASE COUNT          496 a         1141 c         1142 g          499 t
ORIGIN      
        1 aggtcgatgt cggcgtgaac gacgcccgct tgcttcatcg cttgctgcgt cgaggtgctc
       61 accgccgtga gctcgagagg gtcggagcgg cgcgcgagcg cgagcggcgc cgttgcaacc
      121 gccgagccgg cgatgcgcac gctgtcgcgg tttggcagct cacgcgcaac gtgcggcgcg
      181 gccagaatca cggcgctcgc gccgttgcaa acgggcgaga cgtcgaacag gcggatcggg
      241 tcgctaacga cgcgcgactc gaggtaggtc ggaagatcga gcgctttctt cagcagcgca
      301 ttcggattcg tcagcgcgtt gcgatgcgcc gtgatcgcga acggcgcgaa gcgttccgcg
      361 tcgacgccgt gcttggccat gtacgagcgc atcagcgtcg cgttcagcga caagaacgac
      421 tcgccgcaca cgccttcgag ctcccagtcg gcggccgtcg cgagcgcgcg cgtcgctacg
      481 tcgcgctcga cgtgcgtcat gcgttcgacg ccgcagaccg cgacggcatc gtggatgccg
      541 ccggccacgg cttgataggc catgcgcacc gcggcgccgc ccgaggcgca cgcggcttcg
      601 atcgtgacgg cctcgatgcc ggcgagcccc gtgtagtcgg cgatcagccc gccgagttgt
      661 tgctgctggg ccaggatgcc ggaaagcatg ttgccgacgt acagcgcgcc gaccgtcttc
      721 ggctcgatgc cggcgtccgc gagtgcctcc ttcacggcgg ccgcgccgag ataccggccg
      781 cggctttgcg gatccttgtt gaccgccgtc tgcccaacgc cggcaatgaa cacttctctc
      841 aaactcgagc tccgggacgc tcttccaacg gcgcgcatca tggcaaaggg tcgccgcgct
      901 tgccaatcgc ggagctgtcg ggccgcggag cgcgttccgc atcggctatc atgcggatcg
      961 agcgagaaag gagcgcgcgt tgagcgccaa tgaacgcatc gtcgtcgtcg gcttcggcaa
     1021 catggggcag gccctcgtgc gcggttggct ggcgaagggc cgcggcgcaa gcggcatccg
     1081 agtcgtcgat cccgcggcgt ccgcgcgggc ggccgcgcgc gagctcgaga tcgcggtcgc
     1141 ggatacggtc ggcaccgccg acgtcgtcgt gctcgcggtc aagccgaatc agctcgccga
     1201 cgtgttgccc cattgccgtg cggccgtgcg cccggcgtgc gtgttcttgt cgatcgcggc
     1261 cggcaagacg ctagccgaga tcggcagggg attgggccaa gacgcggcca tcgtgcgtgc
     1321 gatgccgaac acgccggccg ccatcggccg agggatcacg gtcctcgtcg cgaacgccgc
     1381 cgtcagccgc gcgcaacgcg cgctctgcgg cgatctgctt gcggcggtcg gttccgtcgc
     1441 ttggctcgac gacgagcggc acatggacgc cgtgaccgca atctcgggca gcggcccggc
     1501 ctacgtgttc ttgttcatcg agtgcctgga gcaagcggca atcgagctcg ggctcgacgc
     1561 cgctctcgcg aagcacttgg cgctcgccac cgtcgccgga tccggcgcgt acgcggagag
     1621 cgcgcgcgaa ccccccgcgg agctccgccg tcgcgtcacg agcccgaacg gcacgacgca
     1681 agcggcgctc gacgtgttga tggcggagcc cggccagcgc gagctgctcg cgcgtgcgac
     1741 gcgcgcggcg gccgagcgca gccgcgagct cgcgaaggtg taaacgatga ccggctacta
     1801 ctacgacgag ttcgaggtcg gccgcgtgtt caagcacgcg atcaggcgca cgatgacgga
     1861 gaccgacaac gtgctcttca gcacgatgac gcacaatccg gcgccgctgc acatcgatca
     1921 cgagtacatg cgcacgcagt ccgagttcgg caagccgctg atgaacagct tcttcacgct
     1981 gggcctcatc gtcggcatct cgatcaacga cacgacgctc ggcacgacgg tcgcgaacct
     2041 cgggctcgag gacgtacgct ttccggcgcc gctgttcgtc ggcgacaccg tccacgtcga
     2101 gaccgaggtc gtcgcgatgc gcccgagcgc ctcacgcccg cgcaacggga tcttgacgct
     2161 cgagcaccgg ggctataacc agaacgacgt cctggtcgcg gcctgccgcc gcaacgttct
     2221 gatgctgcgc tcgccctgac tcagttcggc gcgaacagca ccgcgatgtg gttcgcgtgc
     2281 agcgtcgcgc tcccggcttc gtagcggccg acggccgtca gcgcgatggc gggtcgcccg
     2341 ccgccgagcc gcgacgcgag ctccgtgctg aactcggcga acgacgtgaa catcttgatg
     2401 tccgcgccga ctgcgatgcc gtagatcgcc cggccgtccg caggcgcgag cgtcggcgcc
     2461 atcggcagcg tcgtcaaatc cacgacgcgc atgccgtcga cgagatgatg ccgcgcaccg
     2521 atgctcggat tgtggatgtc gagcacgagc cctgccgcgc ccatgctcga gaacggcgcg
     2581 gtcgtccccg acacgcccca gccgatgccg agcatcgccg gcaagtcgcg gtggtcgatg
     2641 actgcacggc cctcgaagtc cggcggtgcg ccgccgaacg gccgcacgaa accaatgacg
     2701 ctcgccggct cacccgcggc gacgccggcg aggccgagcg tgcccgtggc gacctcgtag
     2761 tcggcgggat cggcgtcctg cgccggcgtg acgccggtgc cggcgaagtc gaacgcgtcg
     2821 atgccgagcc gatcgatgcc gcgcagattc aggttgagct gccccggcac cacggtcttg
     2881 acggcgccgt gcaggtgcgt cggctgcatg cgcacgcggc cattcgtcgc gtcgagcagg
     2941 gccggcatcg tgtcgctcgc gggctgcgtc aacttgccga atgcgacgat ctgctgaccg
     3001 accgacagcg cgcttgggtc gagcacggcg cccggcgttc ctgtcttcag caccttcgtg
     3061 cccgcgccga cgtcgacgag cacggtgcgg tgaacgcgaa tgacgtgctc gcggtcgact
     3121 gcgaacgcgc ccttcaccgt gaggcgattg ccgctgcgcg agacgatgtt gccgtggatc
     3181 gcgtcgacgc gctcgccgct gacgctgtcg ccggcgtgca cgatctcggc cgtgaacctg
     3241 tggtcgaggc gcgagagcgt gccgaacgcg gccgtcat
//
LOCUS       DASSNN010000781         5719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1001077, whole genome shotgun sequence.
ACCESSION   DASSNN010000781 DASSNN010000000
VERSION     DASSNN010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001077"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFL84_14655"
     CDS             complement(<1..180)
                     /locus_tag="VFL84_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609919.1"
                     /translation="MRYLFVAIIGLVLGVAGAGAALYYNPLAEKSAATPGATGRVLHY
                     SLPDQVLEFAIGEDAR"
     gene            complement(177..824)
                     /locus_tag="VFL84_14660"
     CDS             complement(177..824)
                     /locus_tag="VFL84_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609920.1"
                     /translation="MKIVFLLALLIGFAGTLAGAHFAPGLRHERLPSKTTVVANGGRA
                     EKFLIRLPADRIAATDGEAGGLRAMGADGVMMMPAQFVAEPLLIEHFKIRDAGGAVIG
                     IAARHWNGTGVEATTTWSVLIPSRGALVMSSRGEAKAALDSALRARGYSAGSDWAGTV
                     TLPMTGDGQGVVAAGTGEFERLLGTYTETWSVAAVDEDGKVSGTIELATITSRLE"
     gene            complement(927..2465)
                     /gene="gshA"
                     /locus_tag="VFL84_14665"
     CDS             complement(927..2465)
                     /gene="gshA"
                     /locus_tag="VFL84_14665"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HET7609921.1"
                     /translation="MKWLRDGGRAKLIRHGLRGVEKESLRVTADGRLSQRPHPRALGA
                     TLTHPYITTDYSEALPELVTPPQRTQWETLQFLCDLHAFIARRLDGELLWPASMPCEL
                     PADDEIPIARYGPSNLGRMKTVYRRGLGYRYGRAMQAIAGVHFNYSLPLDFWPEYEAF
                     AGRSESLRDFRSAELMGLVRNYRRCAWLVTYLNGASPALGRSFRPGGHELLVELDAAT
                     WYAPYATSLRMSDLGYRNKTQGRLSIRANSLAEYVAGMRDAVTTLEPRYEAIGVLVDG
                     EYRQLNANILQIENEYYSTIRPKPSKAVKSRPLVALAESGVDYVEVRTLDLSATDPVG
                     MNQKELRFVEALLIYCLLAESPPITAAEQAEIDARDLTVAREGRRPGLKLVDGGRERP
                     LRELGLELIAGIRDVAELLDDESQGYVAAVDAAAEALRDPDRTPSAALLAALRSERAS
                     FFEYTLALAHSHAAYFRDFALAPAREQALEETARLSLEEAEALPGKDPRPFADYLRDY
                     FSLT"
     gene            2661..2978
                     /locus_tag="VFL84_14670"
     CDS             2661..2978
                     /locus_tag="VFL84_14670"
                     /inference="COORDINATES: protein motif:HMM:NF022459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HET7609922.1"
                     /translation="MSVTLAAVLLFMAAAPAHAFRCGARIITRGDPAAKILQYCGEPA
                     AVTRRLKERPYVVHSGVVVHGALEEVVVEEWTYNLGPRQLMRVVRLEDGYVEDIKHLG
                     YGY"
     gene            complement(2953..3258)
                     /locus_tag="VFL84_14675"
     CDS             complement(2953..3258)
                     /locus_tag="VFL84_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609923.1"
                     /translation="MDDTVEVIALVLGDANERRWRAGQLGRGAQCLVRRPDGRVALQP
                     VASEEEFERTARTTGCFRRGKPIHDPYTHEVIGYEMEQVPLLRAATPEAVSNRTRGA"
     gene            3486..3893
                     /locus_tag="VFL84_14680"
     CDS             3486..3893
                     /locus_tag="VFL84_14680"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET7609924.1"
                     /translation="MLKLKRRIQDSANGPTTYVAASTTITGTITGKGAYVFCGSVEGD
                     CDIEGPLTLAAGARWRGTVKATDIVVAGVVEGDVVAKQRVEILGTARITGSLSGNSIA
                     VAEGAIIAGEIKVMNGAVPTTFQEKRVVELPSH"
     gene            complement(3885..4871)
                     /locus_tag="VFL84_14685"
     CDS             complement(3885..4871)
                     /locus_tag="VFL84_14685"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7609925.1"
                     /translation="MPHPVRAFDPPRGLTHAHVQSLLASWPWRRRWLRRRASGLLAAS
                     RAELVDCGAGVRLLGHFSAQAAEPRGLAVLLHGWEGSAEANYVVSVGGLLYDAGFSVF
                     RLNFRDHGDTFALNRDLFHSCRIDEVVGAFGAITRRYPAERTFVVGHSLGGNFALRAA
                     ARAPAAGLPLTKIVAVCPVLRPASTMRALEDGLWVYRHYFLRRWRRSLLAKAACFPEL
                     YDFGDLKRFRTLTATTEFFATRYASFPDLDSYLNGYAITGDALARLAVPAKIIAAADD
                     PVIPIGDLDDLAATPALSIQVFPRGGHCAFLEDYALRSWVDRAVLAEIEASV"
     gene            4994..>5719
                     /locus_tag="VFL84_14690"
     CDS             4994..>5719
                     /locus_tag="VFL84_14690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7609926.1"
                     /translation="MLATFLNSPLKRRFMTAVLLLAGSIIAALGVLFYLLVSGALSLA
                     AFAWSGLLCLVAGTAAGFWLIRRVVDRFSAPLRAVTASLQGGTSGELDPAGFGDTAEL
                     RELAAAVNAMRTNLRSSTISRDYLDRLLSSMGEALLITNADGKVERANAAAVELFKQD
                     EAALIGKSADELILSNDRRRAEGVASRPREGTVLRPDGTTVSVSYTVANVHDGDAVQS
                     KVYAAHNIDELKRVEQRIRYLART"
BASE COUNT          987 a         1919 c         1903 g          910 t
ORIGIN      
        1 ccgcgcgtcc tcgccgatcg cgaactcgag cacctggtcc ggcaacgaat agtgcagcac
       61 gcgccccgtg gcgcccggcg tcgccgccga cttctccgcc agcgggttgt agtaaagagc
      121 ggcacccgcg ccggcgacgc cgagaacgag accgatgatt gcgacgaata ggtatctcat
      181 tcgagccggc tcgtgatcgt cgcgagctct atcgtgccgc tgactttgcc atcctcgtcg
      241 acggcggcca cgctccaggt ttccgtatag gtgccgagca gcctttcgaa ctcgccggtg
      301 ccggccgcga cgacgccttg accgtcgccg gtcatcggca acgtgaccgt gccggcccag
      361 tcgctacccg cgctgtagcc gcgtgcgcgc agagccgagt cgagcgcggc tttggcctcg
      421 ccgcgagagc tcatgacgag cgcgccgcgg ctcgggatca aaaccgacca cgtcgtcgtc
      481 gcctcgacgc cggtgccgtt ccaatggcgc gcggcaatgc cgatcacggc gccgcccgca
      541 tcgcgaatct tgaaatgctc gatcagcagc ggctcggcga cgaactgcgc cggcatcatc
      601 atcacgccgt cggcgcccat ggcgcgcagc ccgcccgcct cgccgtcggt cgcggcgatg
      661 cggtcggccg gcaagcgaat caggaacttc tcggcccgcc cgccgttcgc gacgaccgtc
      721 gtcttcgacg gcagccgctc gtgcctgagt cccggcgcga aatgcgcgcc ggcgagcgtt
      781 ccggcgaagc cgatcagcag cgccaataaa aagactatct tcatgcccgt tccgaatggc
      841 cgaaggtgtc agtatccgcg ccggacggcc ggcgcgacgg ttaacggaat cacaaaaatg
      901 ggggcgggtc gattgcccgc cccggcctag gtcagcgaaa aatagtcccg taggtagtcc
      961 gcaaacgggc gcggatcctt gcccggcagc gcttccgcct cctcgagcga caggcgcgcg
     1021 gtttcctcga gtgcctgctc ccgggccggc gccagcgcga aatcgcggaa gtacgcggcg
     1081 tggctgtgcg caagcgcgag cgtgtactcg aagaagctgg cccgctcgct gcgcaaggcc
     1141 gcgagcagcg cggccgatgg cgtgcgatcg gggtcgcgca gcgcctcggc cgcggcgtcg
     1201 acggccgcga cgtagccttg gctctcgtcg tcgagcagct cggcgacgtc gcggatgccg
     1261 gcaatgagct cgagcccgag ctcacgaagc ggccgctcgc ggccgccgtc gacgagcttt
     1321 agacccggcc gccggccttc acgcgcgacg gtgagatccc gcgcgtcgat ctccgcctgc
     1381 tcagccgccg tgatcggcgg gctctcggcc agcagacagt agatgagcag agcctcgacg
     1441 aagcgcagct ccttctgatt catcccgacc ggatccgtcg cgctcaaatc gagcgttcgc
     1501 acctcgacgt agtcgacgcc gctttccgcg agcgcgacga gcggccgcga cttcaccgct
     1561 ttgctcggct tcggacggat cgtgctgtag tactcgttct cgatctgcag gatgttcgcg
     1621 ttgagctgcc ggtactcgcc gtcgacgagc acgccgatcg cttcgtaacg cggctcgagc
     1681 gtcgtgacgg cgtcgcgcat gccggcgacg tactcggcga gcgagttggc gcggatgctc
     1741 aagcgccctt gcgtcttgtt gcgataaccg agatcgctca tgcgcagtga agtcgcgtac
     1801 ggcgcatacc acgtcgccgc gtcgagctcg acgagaagct catggccgcc gggccggaac
     1861 gagcgcccga gcgccggcga agcgccgttc agataggtca cgagccacgc gcaccgccgg
     1921 tagttgcgca cgaggcccat gagctcggcc gagcggaaat cgcgcagcga ttccgaacgg
     1981 ccggcgaagg cctcgtactc gggccagaag tcgagaggca acgaatagtt gaaatgcacg
     2041 ccggcgatgg cctgcatcgc gcgtccgtag cgatagccca agccgcggcg atagacggtc
     2101 ttcatgcggc ccaaattcga ggggccgtag cgcgcgatcg gtatctcgtc gtcggcggga
     2161 agctcgcaag gcatgctggc cggccacagc aactcgccgt cgaggcgccg cgcgatgaac
     2221 gcgtgcaagt cgcacaggaa ctgcagcgtt tcccattggg ttcgctgcgg cggggtcacg
     2281 agctccggca gcgcctcgga atagtcggtc gttatgtaag gatgggtcag cgtcgcgccg
     2341 agcgcgcgag gatgcggccg ctgcgacagc ctgccgtcgg ccgtgacgcg caggctctcc
     2401 ttttctacgc cgcgcaagcc gtgccgaatg agcttcgcgc ggccgccgtc gcgcagccac
     2461 ttcatgcgtg tcgcgaacgg cccgcctcgg gcaggcaacg aagatgacgg gctcgggctc
     2521 gacacgctgc gaacaacttg aatgtgacgc cttcattata ggcgcgcgat tgccgtttga
     2581 gccgattcgg gttaggttcg agcgcagtcg gccggctcga agatttcgag gaggcttacc
     2641 gagcgatggc gtcgaagagc atgagcgtaa cgctcgccgc cgtgctgcta ttcatggccg
     2701 cggctccggc gcacgcgttt cgctgcgggg cacgcatcat cacgcgcggc gatcccgccg
     2761 cgaagatcct gcagtattgc ggcgagcccg ccgccgtcac gaggcggctc aaggagcggc
     2821 cgtatgtcgt gcactccggc gtcgtcgtgc acggagcgct cgaggaagtc gtcgtcgagg
     2881 aatggactta caacttagga ccgcgccagc tcatgcgggt cgtgcggctc gaggacggct
     2941 acgtcgagga tatcaagcac ctcgggtacg gttactgacg gcttcgggcg tcgccgctcg
     3001 cagcagcggc acttgctcca tctcgtagcc gatgacctcg tgcgtgtacg gatcatggat
     3061 cggcttgccg cggcggaagc agcccgtcgt gcgagccgtc ctctcgaact cctcttccga
     3121 cgccaccggc tgcagcgcga cgcggccgtc cggccggcgc acgagacatt gcgcgccgcg
     3181 gccgagctgc ccggctcgcc agcgccgctc gttcgcgtcc ccgagcacca gtgcgatgac
     3241 ctcaacggtg tcgtccatgg tcacttctcg ccgtccgccg caacaatgca gacgggcaag
     3301 caatcgccgt gccaatcgtt cgtttgctgt gcaaacaaac gcttacggct cggccgcgtg
     3361 agcgccgtca caactcacgt gcgacaaaat ttgccacttt gcgtccaatc gtcgctgcgt
     3421 gacctggttc ccgcgtggga cgcgcacgtt cggttacctt ggctgcctgc cgtggagaca
     3481 actcaatgct gaagctcaag cgaagaattc aagacagcgc caacggcccg acgacctatg
     3541 tcgcggcgtc gacgacgatc accggcacga tcaccggcaa gggtgcctat gtcttttgcg
     3601 gctcggtcga aggcgactgc gacatcgaag ggccgttgac gctcgccgcc ggcgcacggt
     3661 ggagaggcac ggtgaaagcc accgatatcg tcgtggccgg cgtcgtcgaa ggcgacgtcg
     3721 tcgcgaagca gcgtgtcgag atcttgggca cggcgcgcat caccggcagc ttgtcgggca
     3781 attcgatcgc ggtcgccgaa ggcgcgatca ttgccggcga gatcaaagtc atgaacgggg
     3841 ccgtgccgac gacattccag gaaaagcgcg tcgtcgagct tccgtcacac tgaggcttct
     3901 atctcggcca gcacggctcg gtcgacccag ctgcgcagcg cgtagtcctc gaggaaggcg
     3961 caatgcccgc cgcgcgggaa cacctgtatc gatagcgcgg gcgtcgccgc gagatcgtcg
     4021 agatcgccga tcgggatcac gggatcgtcc gcggccgcga tgatcttcgc cggcaccgcg
     4081 agtcgcgcga gcgcgtctcc cgtgatcgca tagccgttca agtacgagtc gagatccggg
     4141 aagctcgcgt agcgcgtcgc gaaaaactcc gtcgtcgcag tcaatgttcg aaaccgcttg
     4201 agatcgccga aatcgtacag ctcggggaag cacgcggcct tcgcgagcaa gctgcgccgc
     4261 cagcgccgca agaagtagtg gcggtatacc caaaggccgt cctcgagcgc gcgcatcgtg
     4321 ctcgccggcc gaagcacggg gcacacggcg acgatcttcg tcaaaggaag ccctgcggcc
     4381 ggagcgcgcg cggcggcgcg cagcgcgaaa ttgccgccga gcgagtggcc gacgacgaac
     4441 gtgcgctcgg ccgggtagcg ccgcgtgatc gcgccgaatg ccccgacgac ctcgtcgatc
     4501 cggcacgaat ggaacagatc gcgattcaac gcgaaggtgt cgccgtggtc gcggaagttg
     4561 agccggaata cggagaagcc ggcgtcgtag agcaggccgc cgaccgatac gacgtaattg
     4621 gcctccgcgc tgccttccca gccgtgcagc aagaccgcca gacctcgcgg ttcggcggcc
     4681 tgcgcgctga aatgaccgag caagcgcacg ccggcgccgc aatcgacgag ctcggcacgc
     4741 gacgcggcca gtaggcccga tgcgcggcgg cggagccagc gccttcgcca cggccaactc
     4801 gccagcagcg actggacgtg tgcgtgcgta aggccgcgcg gcggatcgaa tgctcgaacg
     4861 ggatgcggca agcacggcct cgtaatagac tgacggggac ttgcaaggac gagttggcct
     4921 acgcagtagc tccactcgcc ttctacgaat aacaataact aaatagtaga acgagtcgag
     4981 tccgctacta cccatgctcg cgacgttttt gaattcgccg ctgaagcgcc gtttcatgac
     5041 ggcagtcctg ctgcttgcag gatccatcat cgcggcgctc ggcgtgttgt tctatctgct
     5101 cgtgtcgggc gcgctgtcgc tcgcggcctt cgcttggagc gggctcttgt gcctcgtcgc
     5161 gggcacggcc gccggattct ggctcattcg gcgcgtcgtc gaccgtttct ccgcgccgtt
     5221 gcgtgccgtc accgcttcgc tgcaaggcgg cacgagcggc gagctcgacc ccgccggatt
     5281 cggcgacacg gcggagctgc gcgagctcgc ggccgccgtc aatgcgatgc gcacgaattt
     5341 gcgctcgagc acgatctcgc gcgactacct cgatcgcctg ctctcgagca tgggcgaggc
     5401 gctgctgatc acgaacgccg acggcaaggt cgagcgagcg aacgcggccg cggtcgagct
     5461 gttcaagcaa gacgaagcgg cactgatcgg caagagcgcc gacgagctga tcctctccaa
     5521 cgatcgccgc cgcgccgaag gcgtcgcgtc acggccgcgt gaaggcacgg tgctcagacc
     5581 cgacggcacg accgtcagcg tctcgtacac cgtggcaaac gtgcacgacg gcgatgcggt
     5641 gcagagcaag gtttacgcgg cccacaacat cgacgagctg aaacgcgtcg agcagcgcat
     5701 ccgctacctc gcgcgcacg
//
LOCUS       DASSNN010000782         3712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_373463, whole genome shotgun sequence.
ACCESSION   DASSNN010000782 DASSNN010000000
VERSION     DASSNN010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373463"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..585
                     /gene="secB"
                     /locus_tag="VFL84_14695"
     CDS             85..585
                     /gene="secB"
                     /locus_tag="VFL84_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET7609927.1"
                     /translation="MAEQPTPAPQKQLLLQKIYVKDLSFESPMAPLVFTQNVSPQTQL
                     NVRSSAQQVAPDTQEVTLTITVEAKDKDSTLFLAEVAQSGIFVIQGYSAEEQQILIGS
                     FCPNTLYPFAREAISDLVTKGGFPQLLLQPLNFDAIFAQAVQERATRAAAGGQPAPAA
                     AGGEKH"
     gene            605..1582
                     /locus_tag="VFL84_14700"
     CDS             605..1582
                     /locus_tag="VFL84_14700"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET7609928.1"
                     /translation="MVGAGSWGTALAVQFARAGHPAALWARDPGQAAAMQRERRNARY
                     LADTRFPDLLEPTADLAAAVTGADDVLVAVPSHALRETLTELAKLPLRRLAWATKGFE
                     LETGLLPYQVAGQLLPASLPTAVLSGPTFAHEVGAGLPTAMTIASADEDHARRLAERI
                     SSETFRAYTSNDVVGVEVGGATKNVYAIGAGICDGLGFGANTRIALIARGLAEMTRLG
                     EALGGRKETFMGLAGMGDLVLTCTDDHSRNRRFGLKLAAGATPAEALKAIGQVVEGYI
                     GARAVYRVAEKHKVPMPIVVELNKVVHERADPRAAVAALMRRPISSEMG"
     gene            complement(1563..2042)
                     /locus_tag="VFL84_14705"
     CDS             complement(1563..2042)
                     /locus_tag="VFL84_14705"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET7609929.1"
                     /translation="MFHVILYRPEIPPNTGNLIRLCANAGATLHLVHPLGFDLSDAQV
                     RRAGLDYHEMASVREHGDLAACLEAIAPARVFALTTRATRSVYDAAFRSGDAFLFGQE
                     TSGLPQELLDTFAPDMRLRIPMRAGNRSLNLSNAAAVTVYEAWRQLGFAGSTTPSRS"
     gene            2096..3049
                     /locus_tag="VFL84_14710"
     CDS             2096..3049
                     /locus_tag="VFL84_14710"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET7609930.1"
                     /translation="MSRLKLDFCGLELRSPIVLLSGCVGFGEEYTRIDGFNNAAVGAI
                     VLKGTTLAPRLGNPPHRVWETPAGMLNAIGLQNPGVDYVVKSILPTLDFKETRFIANV
                     CGSTIEEYAEVTRRFDDSAIDAMEINISCPNVKEGGVAFGNYPDMSARVVAACRAVTK
                     KPLITKLSPNQTDIRANARACIDAGTDAFAVINTLMGMAIDVETRQPIIGNIQGGLSG
                     PAIRPIALLKVHQVYQECRDRGIPIIGQGGIATAHDALEFVLAGATTVGLGTALFYDP
                     LAPIKVNEGLEEYLRAHRFTSITDLVGGIDPAKALNQAASG"
     gene            3076..>3712
                     /gene="lpxA"
                     /locus_tag="VFL84_14715"
     CDS             3076..>3712
                     /gene="lpxA"
                     /locus_tag="VFL84_14715"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET7609931.1"
                     /translation="MSIHPSVILSDRAKISADAEVGPYCVIEGEVEIGPGTVVESHAR
                     IGSRYGRVTIGANNRIQHGAVLGGPPQDLGYEGGAYTSLTIGSHNRIGEFVSINVGTA
                     KGGGVTRVGDHNFIMAYSHFGHDCQVADHVIIVNGSQFAGHVTIEHHALVSGLVAVTQ
                     FTRLGAYSFMAAGAFANKDIVPYAIAEGHWATPRAVNRVGLKRAGFDAEERR"
BASE COUNT          637 a         1278 c         1236 g          561 t
ORIGIN      
        1 cgacagatcc ggcgagctcg accgcttgct cgcgtcaccg gcaccttagt ttcattcacc
       61 aaaaccaaac gaactggacg aacgatggcc gaacaaccga cccctgcccc gcagaaacag
      121 cttctcctcc aaaagatcta cgtgaaggac ttgtcgttcg agtcgccgat ggcgccactc
      181 gtcttcacgc agaacgtctc gccgcagacg caactgaacg tgcgctcgag cgcgcagcaa
      241 gttgcgccgg atacgcagga ggtcacgttg acgatcaccg tcgaggccaa agacaaggat
      301 tcgaccttgt tcctcgcgga ggtcgcgcaa tcgggcatct tcgtgatcca gggctattcg
      361 gccgaggagc agcagattct gatcggcagc ttctgtccga acacgctcta tccgttcgcg
      421 cgcgaggcaa tctccgatct cgtcacgaag ggcggtttcc cgcagctgct gctgcaaccg
      481 ctgaacttcg acgcgatctt cgcgcaggcc gtgcaagagc gcgcgacgcg cgcggccgcc
      541 ggcggtcaac cggcgcctgc ggcagcgggc ggcgagaagc attagcacgg cgagccgcat
      601 cgcgatcgtc ggggccgggt cgtggggcac ggcgcttgcc gtgcagtttg cgcgcgcggg
      661 gcatccggcg gccctgtggg cgcgcgatcc gggccaggcc gccgcgatgc agcgcgagcg
      721 gcgcaacgcg cgctatcttg ccgacactcg ctttccggat ctgctcgagc ccaccgccga
      781 tctcgcggcg gccgtcacgg gcgccgacga cgtgctcgtc gccgtgccga gccacgcgct
      841 gcgcgagacg ctgacggagc tcgcgaagct gccgctgcgc cggctcgcgt gggcgacgaa
      901 gggcttcgag ctcgagacgg gcctcttgcc gtatcaagtc gccgggcagc tgttgccggc
      961 cagcttgccg acggccgtgc tgtctggacc gacgttcgcg cacgaggtcg gcgccgggtt
     1021 gccgacggcg atgacgatcg cgtcggccga cgaggaccat gcgcggcggc tcgccgagcg
     1081 catcagcagc gagacgtttc gcgcctacac gtcgaacgac gtcgtcggcg tcgaggtcgg
     1141 cggcgcgacg aagaacgtct acgcgatcgg cgccggcatc tgcgacggcc tcggcttcgg
     1201 cgcgaacacg cgcatcgcgc tgatcgcgcg cggcctcgcc gagatgacgc gcttgggcga
     1261 agcgctcggc ggccgcaagg aaacgttcat gggcttggcc ggcatgggcg acctcgtgct
     1321 gacctgcacc gacgatcact cgcgcaaccg ccgcttcggg ctcaagctcg cggcgggcgc
     1381 gacgccggcc gaggcgctca aggctatcgg ccaagtcgtc gagggctaca tcggcgcgcg
     1441 cgccgtctac cgcgtcgcgg aaaagcacaa agtgccgatg ccgatcgtcg tcgagctcaa
     1501 caaggtcgtg cacgagcgcg cggacccgcg cgctgccgtc gcggcgctga tgcgccggcc
     1561 gatcagctcc gagatggggt agtgctgccg gcgaaaccga gctgccgcca cgcttcgtag
     1621 acggtcacgg ccgccgcgtt cgacaggttc aggctgcggt tgccggcgcg catcgggatc
     1681 cgcaagcgca tgtccggcgc gaacgtatcg agcagctcct gcggcaaacc gctcgtctcc
     1741 tgcccgaaca agaacgcgtc gccggaccga aacgccgcgt cgtagacgga gcgcgtcgcg
     1801 cgcgtcgtca gcgcgaacac gcgcgccggc gcaatcgcct cgaggcatgc agcaagatcg
     1861 ccatgctcgc gcacgctcgc catctcgtga tagtcgaggc ccgcgcgccg aacctgggca
     1921 tcgctcaggt cgaagccgag cgggtgcacg aggtgcagcg tcgcgccggc gttcgcacac
     1981 aaccggatca ggttgcccgt gttcggcggg atctccggcc gatacagaat cacgtgaaac
     2041 atcgggcgca tcataggtgg cactccgccc gggcgtcgcg gataattgcg ccccgatgag
     2101 ccggctaaag ctcgatttct gcggtcttga gctccgctcg ccgatcgtgc tgttgtcggg
     2161 ctgcgtcggc ttcggcgagg agtacacgcg catcgacggt ttcaacaacg cggccgtcgg
     2221 cgcgatcgtg cttaagggca cgacgctggc gccgcggctc ggcaatccgc cgcaccgcgt
     2281 ctgggaaacg ccggccggca tgctgaacgc gatcggcctg cagaatccgg gcgtcgacta
     2341 cgtcgtcaaa tcgatcctgc cgacgctcga cttcaaggag acgcgcttca tcgccaacgt
     2401 ctgcggctcg acgatcgagg agtacgccga ggtcacgcgc cggttcgacg attccgcgat
     2461 cgacgcgatg gagatcaaca tctcgtgccc gaacgtcaag gaaggcggcg tcgcgttcgg
     2521 caactacccc gacatgtcgg cgcgcgtcgt tgccgcgtgc cgcgccgtga cgaagaagcc
     2581 gctgatcacg aagctctcgc cgaaccagac cgacattcgc gcgaacgcgc gcgcctgcat
     2641 cgacgccggg accgacgcgt tcgccgtcat caacacgctg atgggcatgg ccatcgacgt
     2701 cgagacacgt caaccgatca tcggcaacat ccagggcgga ctgtccggac cggcgattcg
     2761 gccgatcgcg ctgctcaagg tccatcaggt ctatcaagag tgccgggacc gcggcatccc
     2821 gatcatcggc caaggcggca tcgcgacggc gcacgacgcg ctcgagttcg tgctcgccgg
     2881 agcgacgacg gtcggcctcg gcaccgcgct cttctacgat ccgctcgcgc cgatcaaggt
     2941 caacgaaggg ctcgaggaat acctgcgcgc gcaccgcttc acgagcatca cggacctcgt
     3001 cggcggcatc gatcccgcga aagcgctgaa ccaggccgcc agcggctgac gcctcaccag
     3061 ctttaggcgc ccgcgttgag cattcacccg agcgtaatcc tgagcgaccg cgccaagatc
     3121 tccgccgatg cggaagttgg gccgtactgc gtcatcgagg gcgaggtcga gatcgggccc
     3181 ggcacggtcg tcgaatcgca cgcgcgcatc ggcagccgct acgggcgcgt cacgatcggc
     3241 gcgaacaacc gcattcagca cggcgccgtg ctcggcgggc cgccgcaaga cttgggttac
     3301 gagggcggcg cgtacacgtc gctcacgatc ggctcgcaca acaggatcgg cgagttcgtc
     3361 agcatcaacg tcggcaccgc gaaaggcggc ggcgtcacgc gcgtcggcga ccacaatttc
     3421 atcatggctt actcgcactt cggccacgat tgccaagtcg ccgatcacgt catcatcgtc
     3481 aacgggtcgc agttcgcggg ccacgtcacg atcgagcatc acgcgctcgt cagcggcctc
     3541 gtcgccgtca cgcagttcac gcggctcggc gcgtactcgt tcatggccgc cggcgcgttc
     3601 gcgaacaagg acatcgtgcc gtacgcgatc gccgagggtc attgggctac gccgcgcgcc
     3661 gtgaatcgcg tgggcttgaa gcgcgccggg ttcgacgccg aggagcgccg ca
//
LOCUS       DASSNN010000783         5639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1001229, whole genome shotgun sequence.
ACCESSION   DASSNN010000783 DASSNN010000000
VERSION     DASSNN010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001229"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFL84_14720"
     CDS             complement(<1..444)
                     /locus_tag="VFL84_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HET7609932.1"
                     /translation="MKSKWGAFAALAATVSPAFAGAQQIDSGSTAWMLTSTALVLLMT
                     LPGLALFYGGLVRTRNVLSILMQCFALCCVISLIWVVFGYSLVFGEGTGWIGGLSKLW
                     MGGVELDSVHPVAPAIPETVFSMFQLTFAIITPALIIGGFAERTRF"
     gene            complement(469..807)
                     /locus_tag="VFL84_14725"
     CDS             complement(469..807)
                     /locus_tag="VFL84_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET7609933.1"
                     /translation="MKLITAVVKPFRLDDVRNALAEVGVQGMTVTEVKGFGRQRGHTE
                     LYRGAEYVVDFLPKVKVEVAVADELVDRVIEAIIGAAKTGKVGDGKIFVTELLQVYRI
                     RTGETGDQAI"
     gene            960..1913
                     /gene="msrP"
                     /locus_tag="VFL84_14730"
     CDS             960..1913
                     /gene="msrP"
                     /locus_tag="VFL84_14730"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011048955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HET7609934.1"
                     /translation="MRKRFDKDIKPSEITPEGAYLNRRALLATAFAAGLVPSIMSEAA
                     TLPKSGDLFQDVKKWPDSTTEKVNSFQDITTYNNFYEFGTGKGDPAANAHTLKTSPWS
                     VQVSGEAAKTGTFTLEDILKPHALEERIYRHRCVEAWSMVIPWVGFPLADLLKRFEPT
                     SNAKYVQFVTLNDRTQMPGVRYPVLEWPYREGLTMAEAMHPLSFMAVGLYGKVLPNQN
                     GAPLRTVIPWKYGFKCCKSIVRIHFTDRQPQTSWNMAAPQEYGFYSNVNPQVDHPRWS
                     QASERRLGGGLFAARIPTQMFNGYADEVASLYTGLDLRKNY"
     gene            1935..2639
                     /locus_tag="VFL84_14735"
     CDS             1935..2639
                     /locus_tag="VFL84_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HET7609935.1"
                     /translation="MTQRTVVIVKIAIWAGCLLPLGLLVLRAFGYLGGLGANDVETVL
                     NVCGKTGLNLLLLTLCVTPIRRSTGINKLVVFRRLLGLFAFFYLSLHFLTYALLDLGL
                     LYPLLYGPTAAGVTPAQQLLADIAERPYITIGFTALVLLVPLAVTSTRGMQRRLGRNW
                     VKLHRLVYVVAVLGVIHYWWQIKSGRDAQPLPYAVLLAVLLGVRLRHWLVHSKAAVAP
                     SNQRVEPPQAEEKASH"
     gene            complement(2559..3155)
                     /locus_tag="VFL84_14740"
     CDS             complement(2559..3155)
                     /locus_tag="VFL84_14740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HET7609936.1"
                     /translation="MRKQAFLCLVLGAWAGGAAAETVYVTDSLRLGLHAAADTSDKPF
                     DNLISGTPVEVLERNSSFAHVRLADGREGWVKATFLVADKPAAARVLELEAERGKFEN
                     AAAAAKTSQAAAEQQLTDLRRELQATTGSSESIKETIERLERENTAYIERLEAYRHTL
                     PLVWVLPTVVVALVAGFLLGLWWLDALIRRRHGGFRVY"
     gene            complement(3223..5364)
                     /locus_tag="VFL84_14745"
     CDS             complement(3223..5364)
                     /locus_tag="VFL84_14745"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HET7609937.1"
                     /translation="MASSAQANTPAPAGYAPFAQLIKMLLPSARSVALYDTRPELVWC
                     SDGYERPDLRAMVDPERARDAAATRGSVETTKEGLPVFVSALRGASGQPLGSVVVELG
                     GGNSRSTPSMVVSMLRPVLDCLESQLDLERSTLAADRSAGLELLLQAERDSHQETSAL
                     QELLNHCAKELRCVTAALTVPDKNLEILWTADAASAESKLLDRTQKHLLAWARLNNRP
                     MVINRPGAGAAYKILSCPLHDPHGGVLGLVALFRSADAEDFEPRDVRILEFVGRKALA
                     ILDSEYDALTGLPNRLIFERRAQRALDRTAASVLYADIDELEAINQAFGLSAGDEVIQ
                     RVGALIQRAAGPDALVSRLAGDRFAVALPQDDASQGSAVASKILDAVGQLGYLNGTEA
                     MQVSVSIGTAHAPAGERLPHVLAAAELACKRAKADGGARVSIIDNLAALTPAAARQAI
                     AAADLRDALASNQFQLEAQPIVRLGTRGAVAGYELLVRLRNAAGELVSPDRFLEACAQ
                     YGLLPALDRWVLCAAVEALRPHAQSIASAPLFFAINVSAQSLQSRRYAAFALETLASA
                     GLPPRLFCFELKEPAAVADLAGADALIRELTSAGAKVALDDFGSGLSSIAHLKQLPVS
                     FLKIDGEFVRRIGTDRVAESIVSGIARAARMLGIGTIAEHVETAGVAERLRELDVTLG
                     QGFHFARPQPFAETIRVAVAAPAPAEVKTRA"
BASE COUNT          955 a         1901 c         1850 g          933 t
ORIGIN      
        1 gaagcgcgtg cgttccgcga acccgccgat gatcagcgcc ggcgtgatga tggcgaacgt
       61 cagctggaac atcgagaaca cggtctccgg gatcgccgga gccaccggat gtacgctgtc
      121 gagctcgacg ccacccatcc aaagcttcga aagaccgccg atccaaccgg tcccttcgcc
      181 gaacaccagc gaatagccga acaccaccca gatcagagag atcacgcagc acaacgcaaa
      241 gcactgcatc aggatcgaca gcacgttgcg cgtgcgcacg aggccgccgt aaaagagcgc
      301 gaggcccggc aaggtcatca gcaacaccaa cgccgtcgac gtcagcatcc acgcggtcga
      361 gccgctgtcg atctgctgcg ctcccgcgaa cgcgggactc acggtcgccg cgagcgcggc
      421 gaaagcaccc catttgcttt tcatttaacc cccccgggtt gttcttattt agatcgcttg
      481 atcgccggtc tcgccggtgc gaattcgata gacctgcaag agctccgtca cgaaaatctt
      541 gccgtcgccg accttgccgg tcttcgccgc accgatgatg gcttcgatga cgcggtcgac
      601 gagctcgtcc gccacggcga cctcaacctt tacttttggc aagaagtcga cgacgtattc
      661 cgcgccgcgg tagagctcgg tgtgcccgcg ctggcgtccg aaccctttga cttccgtgac
      721 ggtcataccc tgtacgccca cttcagccaa cgcgttacga acgtcatcga gtcgaaaagg
      781 tttgacgaca gccgttatca gcttcatgag tccccctatg aaattgcgac cggacgctta
      841 cgagaagtgt gccccgacga ggggcagccg ttgttgtgta ttgttattac aggggtattc
      901 tagccaagtc gctccactca aggcgacgct cttcgcatcg cgcttcaggc aggtgagaca
      961 tgcgtaaacg attcgacaaa gatatcaagc catccgagat cacgcccgag ggcgcttatc
     1021 tgaaccggcg cgcgctgctc gcaaccgcgt tcgcggcggg gctcgtgccg tcgatcatgt
     1081 ccgaagcggc gacgctgccg aagagcggcg acctgttcca agacgtcaag aagtggcctg
     1141 actcgacgac ggaaaaggtc aacagcttcc aggacatcac gacttacaac aatttctacg
     1201 agttcggcac gggcaagggc gacccggccg cgaacgccca cacgctgaag acgtcgccct
     1261 ggtcggtcca ggtcagcggc gaggccgcga agacgggcac gttcacgctc gaggacatcc
     1321 tgaagccgca tgcgctcgaa gagcgcatct accggcaccg ctgtgtcgag gcgtggtcga
     1381 tggtcattcc gtgggtcggc ttcccgctcg cggatctctt gaagcggttc gagccgacgt
     1441 cgaacgcgaa gtacgtgcag ttcgtgacgt tgaacgaccg cacgcagatg cccggcgtgc
     1501 gttatcccgt gctcgagtgg ccgtaccgcg aagggctcac gatggccgag gcgatgcacc
     1561 cgctctcgtt catggccgtc ggtctctacg gcaaggtcct gccgaatcag aacggcgcgc
     1621 cgttgcgcac ggtgatcccg tggaagtacg gcttcaagtg ctgcaagtcg atcgtgcgca
     1681 tccacttcac ggatcgccag ccgcagacga gctggaacat ggcggcgccg caagagtacg
     1741 gcttctactc gaacgtgaac ccgcaggtcg accacccgcg ctggagtcag gcgtcggagc
     1801 gccgcttggg cggcggactg ttcgcggcgc ggatcccgac gcagatgttc aacggttacg
     1861 ccgacgaggt cgcgagcctc tacacgggtc tcgatcttcg caagaattac tgacgcggtg
     1921 cggccgggcc caggatgacc cagcgcaccg tcgtcatcgt caagatcgcg atctgggcgg
     1981 gatgcctgct cccgctcggg ctgctcgtcc tcagggcgtt cggctacttg ggcgggctcg
     2041 gcgcgaacga cgtcgagacg gtgctcaacg tctgcggcaa gaccgggttg aacctgctgc
     2101 tgttgacgct ctgcgtcacg ccgattcgcc gctcgacggg catcaacaag ctcgtggtgt
     2161 tccgcaggct tctcggccta ttcgccttct tctatctgag cctgcacttc ctgacgtatg
     2221 cgctgctcga cctggggctc ttgtatccgc tgctctacgg gccgacggcg gccggcgtca
     2281 cgcctgcgca acagctgctc gccgacatcg cggaacgccc gtacatcacg atcgggttca
     2341 ccgcgctcgt gctgctggta ccgctcgccg tgacgtcgac gcgcggcatg cagcgccgcc
     2401 tcgggcgcaa ctgggtgaag ttgcatcggc tcgtctacgt cgtcgcggtg ctcggcgtca
     2461 tccactactg gtggcagatc aagtccggcc gcgacgcgca gccgctgccg tacgctgtct
     2521 tgctcgccgt attgctcggc gttcggctgc gccactggct agtacactcg aaagccgccg
     2581 tggcgccgtc gaatcaacgc gtcgagccac cacaggccga ggagaaagcc agccactaac
     2641 gcgacgacga ccgtcgggag cacccagacg agcggcagcg tgtggcggta agcctcgagc
     2701 cgctctatgt acgcggtgtt ttcgcgctcg agccgctcga tcgtctcctt gatcgactcc
     2761 gacgagccgg tcgttgcttg cagctcccgg cgtagatccg tgagctgctg ctccgcggcc
     2821 gcctgcgacg tcttcgccgc cgcggcggcg ttctcgaatt tgccgcgctc ggcttcgagc
     2881 tcgagcacgc gcgcggccgc cggcttgtcg gcgacgagga aggtcgcctt gacccagccc
     2941 tcgcggccat cggccaagcg cacgtgcgcg aagctcgagt tgcgctcgag gacctcgacg
     3001 ggcgtgccac tgatgaggtt gtcgaacggc ttgtcgctcg tatcggccgc ggcgtgcaag
     3061 ccgagccgca agctgtccgt gacgtagaca gtctccgccg ccgcgccgcc ggcccacgcg
     3121 ccgagcacga ggcagagaaa ggcctgctta cgcatccgtc gatccccgac ttgacagaat
     3181 ctgcgcggat tataggcggc gggacgcgcc cgggaatgtg atctacgccc gcgtcttgac
     3241 ttcggccggc gccggcgcgg ccacggccac ccgaatcgtc tcggcgaacg gctgcgggcg
     3301 cgcgaaatgg aagccttgcc cgagcgtgac gtcgagctcg cgcagccgct cggcaacgcc
     3361 ggccgtctcg acgtgctcgg cgatcgtccc gatgccgagc atgcgcgcgg cgcgtgctat
     3421 tcccgagacg atcgactcgg cgacgcgatc cgtgccgata cgccgcacga actcgccgtc
     3481 gatcttcaag aagctcacgg gcagctgctt cagatgagcg atcgagctca aacccgagcc
     3541 gaagtcgtcg agcgcgacct tggcgccggc actcgtcagc tcgcgaatca acgcatcggc
     3601 ccccgcaaga tcggcgaccg ccgccggctc cttgagctcg aagcagaaga ggcgcggcgg
     3661 gagcccggcc gaggccagcg tctcgagcgc gaacgccgcg tatctgcggc tctgcagcga
     3721 ttgcgccgag acgttgatcg cgaagaacag cggcgcggat gcgatcgact gcgcatgcgg
     3781 ccgcaacgcc tcgaccgcgg cgcagagcac ccagcggtcg agcgccggaa gcagtccgta
     3841 ctgcgcgcag gcctcgagaa atctgtccgg cgatacgagc tcgcccgccg cgttgcgcag
     3901 gcgcacgagc agctcgtagc cggctacggc gccgcgcgtc cccaagcgca cgatcggctg
     3961 cgcttcgagc tggaactgat tcgacgcgag cgcgtcgcgg aggtcggccg cggcgatcgc
     4021 ttgccgcgcg gccgccggcg tcagcgccgc gagattgtcg atgatcgaga cgcgcgcgcc
     4081 gccgtcggcc ttcgcgcgct tgcacgcgag ctctgccgcg gcgagcacgt gcggcagccg
     4141 ttcaccggcc ggcgcgtgcg ccgttccgat gctgacggac acctgcatcg cttccgtgcc
     4201 gttcaagtag ccgagctggc cgacggcgtc gaggatcttc gaggcgaccg cgctgccctg
     4261 agacgcgtca tcttgcggca gcgcgaccgc gaagcgatcg cccgcgagcc ggctgaccaa
     4321 cgcgtcgggg ccggcggcgc gctggatcaa cgcgccgacg cgctggatga cctcgtcgcc
     4381 ggcgctcaaa ccgaaagcct ggttgatcgc ctcgagctcg tcgatgtcgg cgtagagcac
     4441 cgacgccgcg gtgcggtcga gcgcgcgttg agcacggcgc tcgaagatga ggcggttggg
     4501 aagtcccgtc agcgcgtcgt actcgctgtc gaggatcgcg agcgccttgc ggcccacgaa
     4561 ctcgagaatg cggacgtcgc gcggctcgaa gtcttccgca tccgccgacc ggaacagcgc
     4621 gacgaggccg agcacgccgc catgcggatc gtgcagcggg caagacagga tcttgtacgc
     4681 cgcgccggct cccggccggt tgatcaccat ggggcgattg ttgagcctcg cccaggcgag
     4741 cagatgcttc tgcgtgcgat cgagcagctt cgactcggcg gacgccgcat cggccgtcca
     4801 caagatctcg aggttcttgt cgggcacggt cagcgcggcc gtcacgcagc gcagctcttt
     4861 cgcgcagtga ttcaggagct cctgcaacgc gctcgtttcc tggtgggaat cgcgctccgc
     4921 ttgcagcagc agctcgagac cggcgcttcg gtccgcggcc agagtgctgc gctcgagatc
     4981 gagctggctc tcgaggcagt cgagcaccgg gcgcagcatg ctgacgacca tcgatggagt
     5041 gctgcgcgag ttaccgccgc cgagctcgac gaccacggag ccgagcggct gcccggacgc
     5101 gccgcgcagc gccgacacga acacgggcag cccttccttc gtcgtctcta cgctgccgcg
     5161 agtcgcggcc gcgtcgcggg cgcgctccgg atcgaccatt gcgcgcaggt cggggcgctc
     5221 gtagccatcc gagcaccaga cgagctcggg gcgagtgtca tagagcgcga cgctgcgggc
     5281 gctcggcagc agcattttga tgagctgcgc gaagggcgcg taccctgccg gcgcgggggt
     5341 gttcgcttga gcgctgctgg ccacgcaata tgtccaatgg gcctgaggtt gccttggaca
     5401 cgatagtgaa ggaggccgcc gcgaaattcc ttgatggcgg tcgcaagtgt tgcgaatcga
     5461 ggaatttttg cctaccgccc cgacaggtag cgcggcgcct tacggggtgg cgccaccacc
     5521 ttcagcggct cggcgcgctc gaggccgccc acgagctcga catagagccg caagatcgtc
     5581 gcggcgtggc cggtggcctc gcgcggcgac atcttctgga cgctggcccg gacgcgcgt
//
LOCUS       DASSNN010000784         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1722167, whole genome shotgun sequence.
ACCESSION   DASSNN010000784 DASSNN010000000
VERSION     DASSNN010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722167"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFL84_14750"
     CDS             <1..598
                     /locus_tag="VFL84_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609938.1"
                     /translation="VLGTRMRVNHDKIAEIEIVWTTTGYWLFNADNYLKYSSSENWGP
                     VPADRRDSRGTLVAAANAYLDAFLEGKIDRVPWGFPCVRVEGGMYTGKDSPSDSCEVG
                     VPSGVNIANRHFVVDEVLGSVVAFCTFGAGGPNGGSGAADTHLFRVENGKLRYVHTIT
                     HLLMADFRRPNQQGQQDQQDQQGAQGQQSRPNRQGQQR"
     gene            complement(609..>2116)
                     /locus_tag="VFL84_14755"
     CDS             complement(609..>2116)
                     /locus_tag="VFL84_14755"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7609939.1"
                     /translation="GFCGTAGAASLATAARDGDVGAVRAQLEAGADVNQLESDGSSAL
                     LWAAYQSSTELVALLLANKADPNRANEFGVTPLLQASRNGDVETMRALLDGGAEIASA
                     MRDGETPLMAAARTGNADAVKLLLERGSDPNGVEALGGQTALMWAAAEGHLGIVDLLL
                     RAGADPNLKARTSELTKRSPRADFPSGGFTALMWAARDGREAMVRRLVEAGADVNLTN
                     GDGATAMMLAIVNDRFDMAATLLTLGADANDGSLWYAVEMRDAPTDWRARDGSRLRPD
                     HPNRLTALDLLERLLAAGADPNKPFTGQMHNASMCCDTKASGTPFFRAAVAADVDAMR
                     LMIAHGADLEWKPAPVEGAPPMPFGDTTGLTPLMVAINGGKGLLMAGGPGDIRESKVG
                     IWREPGDRDPANAVKLLLEAGANPNAVSLKGDTALHIAAHDGKLGPLRELVAGGADVD
                     ARNKDGKTALQLVETMKPRVLDPIAEMVGIFDDGAQPAETSAYLRKLLAVR"
BASE COUNT          305 a          750 c          738 g          323 t
ORIGIN      
        1 cgtgctcggc acgcgcatgc gcgtgaacca cgacaagatc gccgagatcg agatcgtctg
       61 gacgacgacg ggctactggc tcttcaacgc cgacaactac ttgaagtact cgtcgagcga
      121 gaactggggc ccggtcccgg ccgaccgccg cgactcgcgc ggcacgctcg tcgcggccgc
      181 gaacgcgtac ctcgacgcgt tcctcgaagg gaagatcgac cgcgtcccgt ggggatttcc
      241 gtgcgttcgc gtcgaaggag gcatgtacac gggcaaagac tcgccgtcgg acagctgcga
      301 ggtcggcgtc ccgagcggcg tcaacatcgc gaatcggcac ttcgtcgtcg acgaggtgct
      361 gggctcggtc gtcgcgttct gcacgttcgg cgcgggcggg ccgaacggcg gcagcggcgc
      421 ggccgacacg catctatttc gcgtcgagaa cggcaagctc cgctacgtgc atacgatcac
      481 gcatttgttg atggcggatt tccgccggcc gaaccagcag ggtcagcagg atcagcagga
      541 tcagcagggg gcacaaggcc agcagtcccg gccgaatcgg caaggccaac agcggtagcg
      601 ccgagtcgct agcgcaccgc gagcagcttg cgcaggtaag cggacgtctc ggcgggctgc
      661 gcgccgtcgt cgaagatgcc gaccatctcg gcgatcggat cgagcacgcg cggcttcatc
      721 gtctcgacga gttgcagagc cgtcttgccg tccttgttgc gcgcgtcgac gtcggcgccg
      781 ccggcgacga gctcgcgcag cggcccgagc ttgccgtcgt gcgcggcgat gtgcagcgcc
      841 gtgtcgccct tcaagctcac ggcgttcggg ttcgcgcccg cctcgagcaa gagcttgacg
      901 gcgttggccg ggtcgcggtc gccgggctcg cgccagatgc cgaccttgct ctcgcggatg
      961 tcgccgggcc cgccggccat caagagcccc ttgccgccgt tgatcgcgac catcagcggc
     1021 gtcagccccg tggtatcgcc gaacggcatc ggcggcgcgc cttcgacggg cgcgggcttc
     1081 cattcgagat cggcgccgtg cgcaatcatg agccgcatcg catcgacgtc ggccgccacc
     1141 gccgcgcgga aaaacggagt gcccgaggcc ttcgtgtcgc agcacatcga agcgttgtgc
     1201 atctggcccg tgaacggctt gttcggatcc gcgccggccg cgagcagccg ctcgaggagg
     1261 tcgagcgccg tcagccggtt cggatgatcg ggccggagcc gcgagccgtc gcgcgcgcgc
     1321 cagtcggtcg gcgcatcgcg catctcgacc gcgtaccaga gcgagccgtc gttcgcgtcg
     1381 gcgccgagcg tgagcagcgt cgccgccatg tcgaagcgat cgttgacgat cgcgagcatc
     1441 atcgccgtcg cgccgtcgcc gttcgtcagg ttcacgtcgg cgccggcctc gacgaggcgg
     1501 cgaaccatcg cttcccggcc gtcgcgcgcc gcccacatca gcgccgtgaa cccgccgctc
     1561 ggaaaatccg cgcgcgggct gcgcttcgtc aattcggacg tgcgtgcctt caggttcgga
     1621 tcggcgccgg cgcgcagcag caagtcgact atgcccaagt ggccttcggc cgccgcccac
     1681 atcagcgccg tctggccgcc gagcgcctcg acgccgttcg ggtccgagcc gcgctcgagc
     1741 aggagcttca cggcgtcggc gttgcccgtg cgcgcggccg ccatcaacgg cgtctcgccg
     1801 tcgcgcatgg cgctcgcgat ttcggcgccg ccgtcgagca gcgcgcgcat ggtctcgacg
     1861 tcgccgttgc ggctcgcttg cagcagcggc gtgacgccga attcgttggc ccggttcgga
     1921 tccgccttgt tcgcgagcaa cagcgcgacg agctccgtcg aggactgata cgcggcccac
     1981 aggagcgccg agctgccgtc gctctcgagc tgattgacgt cggcacccgc ctcgagctgc
     2041 gcgcgcacgg cgccgacgtc gccgtcgcgc gccgcggtgg cgaggctcgc cgcgcccgcg
     2101 gtgccgcaga agccga
//
LOCUS       DASSNN010000785         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1887757, whole genome shotgun sequence.
ACCESSION   DASSNN010000785 DASSNN010000000
VERSION     DASSNN010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887757"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VFL84_14760"
     CDS             <1..1200
                     /locus_tag="VFL84_14760"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7609940.1"
                     /translation="FDLVLLDIWMPDTDGISLLREWSEGGSTGTVVMMSGHGTVDTAV
                     EATRLGAVDFVEKPVSLAKLLRTVERALAVRRTKGERRGAAVHSSVPAGKSQPVRALR
                     EQLARVAQHNAHTLFTGEPGSGRELFARFLASQSAQARGPFVAVMGGDLTDENAQREL
                     LGDGTEPGAFERADGGILFIQELGEVGAQAQRILLSALEQGSYRVPGQTTDRPFTARI
                     LSSAFPGFERSDTLRPELLSHLSVVVIRVPPLREYPEDVPELLRHHVDLLVDAEGLPF
                     RRFSVAAQNRLRNYPWPRNVRELRNMVKRLLILGGPEEISLSEVEAQLATDTADREPL
                     VKQDLLAMPLREAREHFERAYLQQQLLLCGGKVGQLAKRVGMERTHLYRKLRSLGVDF
                     RQSLTDD"
     gene            complement(1209..1793)
                     /locus_tag="VFL84_14765"
     CDS             complement(1209..1793)
                     /locus_tag="VFL84_14765"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7609941.1"
                     /translation="MARRNRNSHRNQSAPGWVWMLFGLGLGLVVAIGVYLRAPSAARP
                     EAPAETLATAERPASRPASRTTSAQPERGAEAENRFEFYEILPQYEVVVGDDAPATGT
                     ASATANTRRRPAATPGSYLLQVGSYGAAADADRIQASLALLGFESHVQRATLDGNVFN
                     RVRIGPIVDLDAAKRTQRQLRAAGYDPLLMQVPK"
     gene            complement(1840..>2572)
                     /locus_tag="VFL84_14770"
     CDS             complement(1840..>2572)
                     /locus_tag="VFL84_14770"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET7609942.1"
                     /translation="VGAGTQRVGDELRSLFPAARIARLDRDSTSRKGELDAVLNDVET
                     GNVDILVGTQMLTKGHDFPRVTLVGVLNADQGLFGTDPRSHERLAQTILQVAGRAGRA
                     DLPGEVVIQTHHPGHPLLACLVDGDYTAFATLALTERREAAWPPFAHLALWHAEASRR
                     EGALAFLEQVRLAAGAERSSVAVLGPAPLAMERKSGRYRAQLLFRSVERAPLHRLVER
                     TLLAVRGSPAARRARWSVDIDPLEI"
BASE COUNT          373 a          873 c          928 g          398 t
ORIGIN      
        1 ttcgatctgg ttctgctcga catctggatg ccggacacgg acggaatctc gctgctgcgc
       61 gagtggtccg agggcggatc gaccggcacc gtcgtcatga tgtcgggtca cggcaccgtg
      121 gatacggccg tcgaggcgac gcggctcggc gccgtcgact tcgtcgagaa gcccgtctcg
      181 ctcgcaaaat tgctgcgcac cgtcgaacgc gcgctcgccg tccgccgaac gaaaggcgaa
      241 cgccgcggcg cggccgtgca cagctcggtt cccgcgggca agagccagcc ggtgcgtgcg
      301 ctgcgcgagc agcttgcgcg cgtcgcgcag cacaatgcgc acacgttgtt cacgggcgag
      361 cccggcagcg gccgcgagct gttcgcacgt tttctcgcga gccagagcgc gcaagcgcgc
      421 ggcccgttcg tcgccgtgat gggcggcgac ctgaccgatg agaacgcgca gcgcgagctg
      481 ctcggcgacg gcacggaacc gggcgcgttc gagcgcgccg acggcggcat ccttttcatc
      541 caggagctcg gcgaagtcgg cgctcaggcg cagcggattt tgttgagcgc gctcgagcag
      601 ggcagctatc gcgtgccggg gcagaccacg gatcggcctt tcaccgcgcg gatcctgagc
      661 agcgcgttcc cggggttcga gcgcagcgac acgctgcgtc ccgagctgct gtcgcacttg
      721 agcgtcgtcg tgatccgggt gccgccgctg cgcgagtatc ccgaggacgt tcccgagctg
      781 ctgcgccatc acgtcgactt gctcgtcgac gcggaggggc tgccgtttcg gcgcttcagc
      841 gtcgcggcgc agaacaggct gcgcaactac ccgtggccgc gcaacgtgcg cgagctgcgc
      901 aacatggtca agcgcctgtt gatcctcggc ggaccggaag agatctcgct ctcggaggtc
      961 gaagcgcagc tcgcgacgga caccgcggac cgcgagccgc tcgtcaagca ggatttgctc
     1021 gcgatgccgc tgcgcgaggc gcgcgagcac ttcgagcgcg cctatctgca acagcagctg
     1081 ctgctatgcg gcggcaaggt cggccagctc gccaagcgcg tcggcatgga gcgcacgcat
     1141 ctctatcgca agctgcgttc tctcggcgtc gattttcgcc agtcgttgac cgacgactga
     1201 gcttcggatt atttcgggac ctgcatcagc agcggatcgt agccggccgc gcgcagctgg
     1261 cgctgcgtgc gcttggcggc gtcgaggtcg acgatcggcc cgattcggac gcggttgaag
     1321 acgttgccgt cgagcgtggc gcgttgcacg tgcgactcga agccgagcag ggcgagactc
     1381 gcttgaatgc ggtctgcgtc ggcagctgcc ccgtacgagc cgacttgaag caagtagctg
     1441 ccaggcgtcg cggcgggtcg tcgccgcgta ttcgccgttg cgctcgccgt gcccgtcgct
     1501 ggagcgtcgt cgccgacgac gacctcgtac tgcggcagga tttcgtagaa ctcgaagcgg
     1561 ttctcggcct cggcgccgcg ctcgggctgt gccgacgtgg tgcgggacgc cggccgcgag
     1621 gccggccgtt cggcggttgc gagcgtctcg gccggtgcct caggccgcgc ggccgacggc
     1681 gcgcgcagat agacgccgat cgcaacgacg agccccagtc ccaacccgaa cagcatccaa
     1741 acccagccgg gcgcggattg attcctgtgc gagttgcgat tgcgtcgtgc caccagtggg
     1801 cgcccattct aaccgagcgc gcgccgcggc ggaggccggt cagatttcga gcggatcgat
     1861 gtcgacgctc cagcgcgcgc ggcgtgcggc cggcgaaccg cgcacggcga gcagcgtgcg
     1921 ctcgacgagc cgatgcagcg gggcgcgctc cacgctgcgg aacaacagtt gcgcccggta
     1981 gcgaccgctc ttgcgctcca tcgcgagcgg cgccgggccg agcacggcga ccgagctccg
     2041 ctccgcaccc gcggcgaggc gcacctgctc gaggaatgcg agggcgcctt cgcggcgcga
     2101 ggcctcggcg tgccacaacg cgaggtgcgc gaacggcggc cacgctgcct cgcggcgttc
     2161 ggtcagcgcg agcgtcgcga aggccgtgta atcgccgtcg acgaggcacg ccaagagcgg
     2221 gtgacccggg tgatgagtct ggatcacgac ctcgcccggc aagtcggcgc ggcctgcgcg
     2281 ccccgcgact tgcaggatcg tctgtgcgag ccgctcgtgg ctgcgtgggt cggtgccgaa
     2341 caagccctgg tccgcgttca gcacgccgac gagcgtgacg cgcggaaagt cgtggccctt
     2401 cgtcagcatt tgcgtgccga ccaggatgtc gacgttgccg gtctcgacgt cgttgagcac
     2461 ggcgtcgagc tcgccttttc ggctcgtgct gtcgcggtcg agccgcgcga tgcgcgcggc
     2521 cggaaacagc gagcgcagct cgtcgccgac acgctgcgtg ccggcgccga cg
//
LOCUS       DASSNN010000786         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1002000, whole genome shotgun sequence.
ACCESSION   DASSNN010000786 DASSNN010000000
VERSION     DASSNN010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002000"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VFL84_14775"
     CDS             complement(<1..310)
                     /locus_tag="VFL84_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HET7609943.1"
                     /translation="MKRRCSDERRWAARLAFAFVLPCLAVACASTSLSLPDTQQRIER
                     YIGSGDYDSEFGAVVARARAYLEKRAPQVTKPAIVLDIDETSLTNWPAYRANGWARIT
                     A"
     gene            complement(318..1244)
                     /locus_tag="VFL84_14780"
     CDS             complement(318..1244)
                     /locus_tag="VFL84_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609944.1"
                     /translation="MVRAAVLCAVLVISAPEAARAQQTDAGAELVGTWLLAGAERDVA
                     SGEPRRVAQSRGLLILDGAGNVFEFFTTPSGAPPAQPDARRVFADNGGFWGRYEAFPA
                     ESRIDFAAEDGVSPSVHGLKFSRRYEIAGDRLIVTTTNEPQAQRDVRLTWQRVPTVEN
                     LSPAYREVVGFWRHVEESRLNTATGAVDNVRRRAPSVIVYTPSGFVGVHFPTLNRTPF
                     AGETPTEEEAQAARNYLGYYGALGVYPGEVFHNILGGVSPTAGAILRRFAKVTGDELV
                     VTLPSGNPQSTTATIVRLRRLSGADDMLPRSR"
     gene            complement(1259..1888)
                     /locus_tag="VFL84_14785"
     CDS             complement(1259..1888)
                     /locus_tag="VFL84_14785"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HET7609945.1"
                     /translation="MTRLNLFSTPLLIATLPEADAVNAELKRIILTREAASEAAQRSI
                     PGRWQSSWDMHQWCGAPLKPVLELVRTIVEQLTVDRAGNRHQIAWRIRCRATINREGH
                     GDQFHTHPGALWSATYCVDDGGRDADASLGGELEVQDPRGVATVMYVPYLTFAGEDGA
                     ALGEAHRLPQAAGTALVYPSWLSHGVRPYRGARERISIGMTFGLANVWS"
BASE COUNT          317 a          731 c          710 g          366 t
ORIGIN      
        1 ctgccgtgat ccgtgcccag ccgttcgcgc ggtacgcggg ccagttcgtg agcgaggtct
       61 cgtcgatatc gagcacgatg gccggcttgg tcacctgcgg agcacgcttt tcgaggtacg
      121 cccgggcgcg cgccacgacc gcgccgaatt cgctgtcgta atcgcccgag ccgatgtagc
      181 gctcgatgcg ttgctgcgtg tcgggcaggc tgagcgaagt gctcgcgcac gcaaccgcga
      241 gacacggaag cacgaaagcg aacgcgagcc tcgccgccca tcgtcgctcg tcgctgcaac
      301 ggcgcttcat ctcgcgatca gcgcgagcgc ggcagcatgt cgtccgcgcc gctcagccgg
      361 cgcaagcgga cgatcgtggc ggtcgtcgat tgcgggttgc cgctcggcag cgtcacgacg
      421 agctcgtcgc cggtgacctt cgcgaagcgg cgcaggatcg cgccggcggt cggcgacacg
      481 ccgcccaaga tgttgtggaa cacctcgccc ggatacacgc cgagcgcgcc gtagtagccg
      541 agataattgc gcgcggcctg cgcttcttct tcggtcggcg tttcgccggc gaacggcgtg
      601 cggttcagcg tcgggaaatg cacgccgacg aagccgctcg gcgtgtagac gatgacgctc
      661 ggcgcgcgcc ggcgcacgtt gtcgaccgcg cccgtcgccg tgttcaagcg gctctcctcg
      721 acgtgccgcc agaagccgac gacctcgcgg tatgcggggc tcaagttttc gacggtcggc
      781 acgcgctgcc acgtcaagcg cacgtcgcgc tgcgcttgcg gctcgttcgt cgtcgtgacg
      841 atgagcctgt cgccggcgat ctcgtagcgc cgcgaaaact tcaggccgtg cacgctcggg
      901 ctcacgccgt cctcggccgc gaagtcgatt cgactctcgg cagggaaggc ttcgtagcgc
      961 ccccagaatc cgccgttgtc ggcgaagacg cgccgcgcat cgggctgcgc gggcggcgcg
     1021 ccgctcggcg tagtgaagaa ctcgaacacg ttgcccgcgc cgtcgaggat gagcaggccg
     1081 cgggattgcg ccacgcggcg cggctcgccg ctcgcaacgt cgcgttcggc gccggcgagc
     1141 agccaggtgc cgacgagctc ggcgcccgcg tcggtttgct gcgcccgcgc cgcctctggc
     1201 gccgagatca cgagcaccgc gcacaatacc gctgcgcgca ccataccggt cctcctcttc
     1261 aactccacac gttggccagg ccgaaggtca tgccgatcga aattcgctcg cgcgcgccgc
     1321 gatacgggcg aacgccgtgc gagagccagc tcgggtacac caatgccgtg ccggccgcct
     1381 gcggcagcct gtgcgcctcg ccgagcgcgg cgccgtcttc gccggcaaac gtcaagtacg
     1441 gcacatacat caccgttgca acgccacgcg gatcctgaac ctcgagctcg ccgccgagcg
     1501 acgcgtcggc gtcgcggccg ccatcgtcga cgcagtaagt cgcggaccag agggcgccgg
     1561 gatgagtgtg gaactgatcg ccgtgtcctt cgcgattgat cgtcgcgcgg cagcggattc
     1621 gccaagcgat ctgatgccgg ttgccggccc ggtcgacggt gagttgctcg acgatcgtgc
     1681 gcacgagctc gagcaccggc ttgagcggcg cgccacacca ttggtgcata tcccatgacg
     1741 attgccacct gccggggatc gagcgctgcg cggcttcgct cgcagcttcg cgcgtgagga
     1801 tgatgcgctt cagctccgcg ttgaccgcgt ctgcctcggg tagcgtcgcg atcaggagcg
     1861 gcgtcgagaa cagattcaga cgtgtcattt gcatcgcgtc gattcccgcg ggactccgtg
     1921 atccgccgac cgctccgcgg tgtgccatgc gacttctgct ccgcatcgta tcgtttctgg
     1981 gacagcgaat tcagctgctc ggcatcgtcg cattttcgcc ttgcgcgctt tcgagatgcc
     2041 gtgctagctt cggctctaac ttacggggag gcttggtcta caacaacact gtctgaattt
     2101 cgatgtgcgc ccgcgttcgc acgt
//
LOCUS       DASSNN010000787         3795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_374795, whole genome shotgun sequence.
ACCESSION   DASSNN010000787 DASSNN010000000
VERSION     DASSNN010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374795"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /gene="rpoA"
                     /locus_tag="VFL84_14790"
     CDS             <1..705
                     /gene="rpoA"
                     /locus_tag="VFL84_14790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET7609946.1"
                     /translation="PGPVTAGDIQLDHDVEVLNGDLVIANLTKAGELNMTLKVERGRG
                     YRPATQIAQFEEQTRPIGRLQLDASFSPIRRVTYTVERARVEQRTDLDKLIVDIETNG
                     TIDAEEAIRRAGLILKNQLDVFVDLQGEDEVGFGRTETQVDPILLRPVDELELTVRSA
                     NCLKAENINYIGDLVQRTEVELLRTPNLGKKSLTEIKEVLEQHGLALGMRLDNWPPPG
                     LRSEEPSTPPSSSMAF"
     gene            720..1118
                     /gene="rplQ"
                     /locus_tag="VFL84_14795"
     CDS             720..1118
                     /gene="rplQ"
                     /locus_tag="VFL84_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET7609947.1"
                     /translation="MRHRNSGRRLGRKSPHRRAMYRNLAASLLVHETVRTTLPKAKEL
                     RRVVEPLITMAKEDSVSHRRLAFDRLRDDKAVGVLFKELGPRFKSRPGGYLRILKMGF
                     RAGDSAPMAIVQLLDQPEKVAEPKEPRTTA"
     gene            complement(1136..1588)
                     /locus_tag="VFL84_14800"
     CDS             complement(1136..1588)
                     /locus_tag="VFL84_14800"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET7609948.1"
                     /translation="MPSPRPPTARGVVARLFVDSGAWIALRSRRDQHHAAAELAFGAA
                     VERRIPLVTTNLIVAEVFRLTLFRAGREPALRGLDRVDASPSVTVHFATGDDHGAARR
                     WLERLAPRPITYTDAVSFAVIDATKCTHVLGFDADFEAAGFEPWTPRA"
     gene            complement(1530..1811)
                     /locus_tag="VFL84_14805"
     CDS             complement(1530..1811)
                     /locus_tag="VFL84_14805"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET7609949.1"
                     /translation="MTKPLQVYLEQDDLDRLETFARERGWTKSQVIRTAIRALTRRAA
                     TDPVLELSGDIDALPPDLSAEFDRYLNETFVAEPTTTYRARRRRTTVRR"
     gene            complement(1898..2638)
                     /locus_tag="VFL84_14810"
     CDS             complement(1898..2638)
                     /locus_tag="VFL84_14810"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HET7609950.1"
                     /translation="MADGPRASPALTAEIAGAMASGAGRVLGSGYQASVHLYRTSVGD
                     VVVKLPHRGGPLGAIWRSLLRREHAVYERLHGVAGIPRSFGLVGDGLALEYVAGPSLR
                     EHDGELVDREAFFAKLKATVEAMHERGIAHGDLKRKNNIIVGAGEQPYIIDFGIAVRR
                     SARSASFNRWVFEQLEQMDLNAWVKLKYRRRIDLGTERDALSAEDAAIYKPHWIERLA
                     RAVRVPWQTITLRRPRQRWRARRNGDES"
     gene            complement(2654..3250)
                     /locus_tag="VFL84_14815"
     CDS             complement(2654..3250)
                     /locus_tag="VFL84_14815"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7609951.1"
                     /translation="MSHRTADLPPLTGIAHVIRELRYFEGARQGLAVVLVLLYTVTAE
                     PIPALAAVGLVIGLAGELFRLYASGFIVKNQELATDGAYRFVRHPLYTGNILLVIGFA
                     LASSRWWAIPLALFFFWFYYPTAIEYEDRKLRRIFGAAWETWSSHTPALLPRFGAAPP
                     AAPGNRRWSLAVSNSHGEVLFVAMALICAAWIAWEAFF"
     gene            complement(3264..>3795)
                     /locus_tag="VFL84_14820"
     CDS             complement(3264..>3795)
                     /locus_tag="VFL84_14820"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET7609952.1"
                     /translation="AALIASMEALQQRICAEVKPGVDFVVLHAFTHRQIASLLVEHEL
                     VTCSADEAVATGITHGFLPHGLGHLLGLQVHDAGGHLADETGKKREPPRPDPYLRLTR
                     TLQPGVVTTIEPGLYFIPSLLRVLLAKHESRLNRAAIERLVPFGGIRIEDNVEVTADG
                     HRNLTRDAFAAAATRH"
BASE COUNT          675 a         1270 c         1287 g          563 t
ORIGIN      
        1 ccgggcccgg tcacggccgg cgacattcag ctcgaccacg acgtcgaagt gctgaacggc
       61 gacctcgtga tcgcgaacct gacgaaggcc ggcgagctca acatgacgct caaggtcgag
      121 cgcggccgcg gctaccgccc ggcgacgcag atcgcgcagt tcgaggagca gacgcggccg
      181 atcggccgcc tgcagctcga cgcgtcattc agcccgattc gccgcgtgac gtataccgtc
      241 gagcgcgcgc gcgtcgagca gcgcacggat ctggacaagc tcatcgtcga catcgagacg
      301 aacggcacga tcgacgccga agaggcgatt cgccgcgccg gcctgatctt gaagaatcag
      361 ctcgacgtgt tcgtcgatct tcaaggcgag gacgaggtcg ggttcggccg caccgagacg
      421 caggtcgatc cgatcctgct gcgccccgtc gacgagctcg agctcaccgt gcgctcggcg
      481 aactgcttga aggccgagaa catcaactac atcggcgacc tcgtgcagcg cacggaagtc
      541 gagctgctcc ggacgccgaa cctcggcaag aagtcgctga ccgagatcaa ggaagtgctc
      601 gagcagcacg gcttggcgct cggcatgcgg ctcgacaact ggccgccgcc gggactgcgt
      661 agcgaggagc cgtccacgcc gccgtcctcg tcgatggcgt tctgatctag ggaaaatcga
      721 tgcgtcacag aaattcgggc cgtaggctcg gccgcaagtc cccgcaccgg cgcgcgatgt
      781 accgcaacct cgcggcgtcg ctgctcgtgc acgagaccgt gcgcacgaca ctgccgaagg
      841 ccaaggagct gcgccgcgtc gtcgagccgc tgatcacgat ggcgaaggaa gacagcgtga
      901 gccaccgccg gctcgcgttc gatcgcctgc gcgacgacaa ggccgtcggc gtgctgttca
      961 aggagctcgg tccgcggttc aagagccggc cgggcggtta tctgcggatc ctgaagatgg
     1021 gcttccgcgc cggcgactcc gcgccgatgg cgatcgtgca gctgctcgat caacccgaga
     1081 aggtcgccga gccgaaggaa ccgcgcacga cggcgtaagc cgtggagctc agagctcagg
     1141 cgcgcggcgt ccaaggctcg aagccggccg cctcgaagtc cgcatcgaaa ccgagcacat
     1201 gcgtgcactt cgtggcgtcg atgaccgcga agctcacggc atcggtgtac gtgatgggcc
     1261 gcggagcgag ccgttcgagc cagcggcgcg cagccccgtg atcgtcgccg gtcgcaaaat
     1321 gaaccgtgac gctcgggctc gcgtcaacac ggtcgagccc tcgtaacgcg ggttcgcgcc
     1381 ccgcacgaaa cagtgtcagc cgaaacactt cggcgacgat gaggttcgtc gtgacgagcg
     1441 ggatccgtcg ctcgaccgcc gcgccaaacg cgagctcggc cgccgcgtga tgttggtcgc
     1501 gccggcttcg cagcgcgatc cacgccccgc tatcgacgaa cagtcgtgcg acgacgccgc
     1561 gcgcggtagg tggtcgtggg ctcggcaacg aaagtctcgt tgagatagcg atcgaactcc
     1621 gcgctcaggt cgggcggcag agcgtcgatg tcgccgctga gctcgaggac cggatcggtc
     1681 gcagcacgcc gcgtcagcgc gcggatcgcc gtgcggatta cctgcgactt ggtccagccg
     1741 cgctcgcgcg cgaacgtttc caggcggtcg aggtcatcct gctcgagata cacctgcagc
     1801 ggttttgtca tgagaaaaga gcatatgttc tcatgaacga ttcgtcaatc gtctcatgat
     1861 cgcggaaagc ccttcgtcaa aggcgttggc agtgctctca ggattcatcg ccgttgcgtc
     1921 gcgcgcgcca gcgttggcgc ggccgccgca gcgtgatcgt ctgccacggc actcgcaccg
     1981 cgcgcgcgag ccgctcgatc cagtgcggct tatagatcgc cgcatcttcc gccgatagcg
     2041 catcgcgctc ggtccctaag tcgatgcgcc ggcggtactt gagcttgacc cacgcgttca
     2101 agtccatctg ctcgagctgc tcgaacaccc agcgattgaa cgacgcgctc cgcgcgctgc
     2161 gccgcaccgc gatgccgaag tcgatgatgt acggctgctc gccggcgccg acgatgatgt
     2221 tgttcttgcg cttcaggtcg ccgtgcgcga tgccgcgctc gtgcatcgcc tcgaccgtcg
     2281 ccttgagctt cgcgaagaaa gcctcgcggt cgacgagctc gccgtcgtgc tcgcgcagcg
     2341 acggtccggc cacgtactcg agcgccaagc cgtcgccgac gagaccgaac gagcgcggaa
     2401 tgcccgcgac gccatggagg cgctcgtaaa cggcgtgctc gcgccgcagc agcgaccgcc
     2461 atatcgcgcc gagcggtccg ccgcggtgcg gcagcttgac gacgacgtcg ccgacggaag
     2521 tgcggtagag gtgcaccgac gcttgatacc cgctgccgag cacgcggccc gcgccggaag
     2581 ccatggcgcc ggcgatctcg gccgtcaatg caggagacgc tcgggggccg tcagccacgg
     2641 ccgctcgtgt cggtcagaag aacgcttccc acgcgatcca cgcggcgcag atcagcgcca
     2701 tcgcgacgaa cagaacctcg ccgtgcgagt tgctgacggc gagcgaccag cgccgattgc
     2761 ccggcgcggc aggcggcgcg gcgccgaagc gcggcagcag cgccggcgtg tgcgaagacc
     2821 acgtctccca ggccgcgccg aagatgcggc gcagcttccg gtcctcgtat tcgatcgccg
     2881 tcgggtagta gaaccagaag aagaacaacg cgagcgggat cgcccaccag cgcgagctcg
     2941 cgagcgcgaa accgatgacg agcaagatgt tgcccgtgta cagcgggtgg cgcacgaagc
     3001 ggtacgcgcc atcggtcgcg agctcctgat ttttgacgat gaagccgctc gcatacaaac
     3061 gaaacagctc gccggcgaga ccgatgacca acccgaccgc cgcgagcgcc gggatcggct
     3121 cggccgtcac cgtgtagagc agcacgagca cgacggcgag cccttggcgc gcgccctcga
     3181 aataccgaag ctcgcgtatg acgtgcgcga ttccggtcag aggcggaagg tcggcggtgc
     3241 gatgagacat cggcgaggag ctcctagtga cgtgtcgcgg ccgccgcgaa cgcgtcgcgc
     3301 gtgaggttgc gatgaccgtc cgccgtgacc tcgacgttgt cctcgatgcg aatgccgccg
     3361 aacggcacga gccgctcgat cgcggcgcga ttgaggcgcg actcgtgctt cgcgagcagc
     3421 acgcgcagca acgacgggat gaagtagagg ccgggctcga tcgtcgtgac gacgccgggc
     3481 tgcagtgtgc gcgtcaaccg caagtacggg tcgggcctcg gcggctcgcg cttcttgccg
     3541 gtctcgtccg cgagatggcc ccccgcgtcg tgcacctgaa gaccgagcag gtggccgagg
     3601 ccgtgaggca gaaagccgtg cgtgatgccg gtcgcgacgg cctcgtcggc gctgcaggtc
     3661 acgagctcgt gctcgaccag caacgaggcg atctgccgat gcgtgaatgc atgtagcacg
     3721 acgaagtcga cgcccggctt tacttccgcg cagatgcgct gctggagcgc ctccatcgac
     3781 gcgattagcg ccgcg
//
LOCUS       DASSNN010000788         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_880311, whole genome shotgun sequence.
ACCESSION   DASSNN010000788 DASSNN010000000
VERSION     DASSNN010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880311"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VFL84_14825"
     CDS             <1..1243
                     /locus_tag="VFL84_14825"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7609953.1"
                     /translation="VLLAAGAKPDLTNRTGASPLYLAATNGDAAAIARLLDAGEDANA
                     VLTAEGETVLMLTAHTGNAEAVKLLLDRGADANAQQVRGQTALMWAAAEGHAAVVKLL
                     LARGADPALSSAASTKQEKRPPGGMTPLLFASRQGHLGAVRALLDGGAAVDQAGADNT
                     SPLLIAIVNGHYGVASLLLERGANPNIADSNGRTPLYAAIDLRNVQWSQAPAPELPQA
                     EHLAMITKLLDAGADPSVKMVGLVGHRGSFDMRWTDLKGGNAFSRAAWNGDIEVMRLL
                     LARGADPRVVTEKGETALLLLAGAGWPLGQGYIRSEGEIRAALDLLVEQLGMDVNAAT
                     TEGITPVICAIFKGDNFVVQYLVDHGARLDVKDAKGRGVIEWAQGVDANGGQPPRPQP
                     ETEKLVRELMAKQGITIARAD"
     gene            complement(1357..1737)
                     /locus_tag="VFL84_14830"
     CDS             complement(1357..1737)
                     /locus_tag="VFL84_14830"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609954.1"
                     /translation="MKLKITSAALAASVVLVAARPLAAHHSAAMFDETKVVELSGTVK
                     ALQWTNPHVWLQVMVGEKGSATEWSFEGGSPNSLSRQGWRSTTFKPGDVVTVRMRPMK
                     DGTAAGQFIGAKFAADGHTVGRWE"
     gene            complement(1754..2518)
                     /locus_tag="VFL84_14835"
     CDS             complement(1754..2518)
                     /locus_tag="VFL84_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609955.1"
                     /translation="MTVETARGQAPAALPDWSGVWAMQGPTVFDRSTVRPADGRTGQP
                     GVREFPPYNEEWEAIYRRNIELVREGNFPDPISACGIPHGFPRIMNVPDVYEFAVTRD
                     AVYILAENGPGIMRIYTDGRAYPPAEELWPTYTGASIGHWEGDTLVFTTISLKGSSGN
                     DVILDRTGLVLSDASRATTRMRKIDDATIEAQMTIEDDKALTRPWQVTKRYTRQPPGT
                     WAWDYSCGENNRNPVAPSGKTLTLGPDGKPLDRVTE"
BASE COUNT          462 a          959 c          940 g          477 t
ORIGIN      
        1 cgtgctgctc gcggccggcg cgaagcccga tctcacgaat cgtaccggtg cgagcccgct
       61 gtacctcgcc gcgactaacg gcgacgccgc cgcgatcgcg cgcctgctcg acgcaggcga
      121 agacgccaat gccgtgctga cggccgaggg cgagaccgtg ctgatgctca ccgcgcacac
      181 cggcaacgcc gaagccgtga agctattgct cgatcgcggc gccgacgcga acgcgcagca
      241 agtgcgcggc cagacggcgc tgatgtgggc ggcggccgaa gggcacgccg ccgtcgtgaa
      301 gctgctgctc gcgcgcggcg cggatcccgc gctgtcgtcc gcggcttcga cgaagcagga
      361 gaagcggccg ccgggcggca tgacgccgct attgttcgcg tcgcgccaag gccatctcgg
      421 cgccgtgcgc gcgctgctcg acggcggcgc cgcggtcgat caggcgggcg cggacaacac
      481 gagcccgctc ttgatcgcca tcgtcaacgg ccactacggc gtcgcgtcgc tgctgctcga
      541 gcgcggcgcg aatccgaaca tcgcggactc aaacggccga acgccgctct acgcggccat
      601 cgacttgcgc aacgtgcagt ggtcgcaggc gcccgcgccg gagctgccgc aggccgagca
      661 cctcgcgatg atcacgaagc tgctcgacgc cggggccgat ccttccgtca agatggtggg
      721 gctcgttggg caccgcggct cgttcgacat gcgctggacc gatttgaagg gcggcaacgc
      781 gttctcgcgc gcggcgtgga acggcgacat cgaggtcatg cgcctgctgc tcgcgcgcgg
      841 cgcggacccc agagtcgtca ccgagaaggg cgagacggcg ctgctgttgc tcgcgggcgc
      901 gggttggccg ctcggccaag gctacattcg gtccgagggc gagattagag cggcactgga
      961 cctgctcgtc gagcagctcg gcatggacgt caacgccgcg acgacggaag gcatcacgcc
     1021 ggtcatctgc gcaattttca agggcgataa cttcgtcgtg cagtatctcg tcgaccacgg
     1081 cgcgcgcctc gacgtcaagg acgcgaaggg ccgcggcgtc atcgagtggg cacaaggcgt
     1141 cgacgcgaac ggaggccagc ccccgcggcc gcagcccgag accgagaagc tcgtgcgcga
     1201 gctcatggcg aagcagggga tcacgatcgc gcgcgccgat taggcgcgcg gaaccgctgt
     1261 tgcagctcaa agtcgatgta acgcgccggc cgcgccacgg ctatgagact tttcgacgct
     1321 tcgcgatcag ttcccggacg gtcggcaccc accacgctac tcccagcggc cgacggtgtg
     1381 tccgtccgcg gcgaacttcg cgccgatgaa ctgacccgcg gccgtgccgt ctttcatcgg
     1441 ccgcattcgc accgtgacga cgtcgccggg cttgaacgtc gtgctgcgcc agccttggcg
     1501 cgacagcgaa ttcgggctgc cgccctcgaa gctccattcg gttgcggagc ctttttcgcc
     1561 gaccatcact tgcagccaga cgtgcggatt cgtccactgc agcgccttga ccgtgccgct
     1621 gagctcgacg actttcgtct cgtcgaacat cgcggccgag tgatgagccg cgagcggacg
     1681 cgcggcgacg agcacgacgc tggccgcgag cgccgcagac gtgattttca atttcatctc
     1741 gagcacctcg ccgttattcc gtgacgcgat cgagcggctt gccgtcgggg ccgagcgtca
     1801 gcgtcttgcc cgacggcgcc acgggattgc ggttgttctc gccgcacgag taatcccaag
     1861 cccacgttcc gggcggctgc ctcgtgtagc gcttcgtgac ttgccagggc cgcgtcagcg
     1921 ccttgtcgtc ctcgatcgtc atttgcgctt cgatcgtcgc atcgtcgatc ttgcgcattc
     1981 gcgtcgtcgc gcggctcgcg tcactcaaca cgagccccgt gcgatcgagg atcacgtcgt
     2041 tgccgctcga gcctttgaga ctgatcgtcg tgaacacgag cgtgtcgcct tcccaatgac
     2101 cgatcgaggc gccggtatag gtcggccata gctcctcggc cggcggatac gcgcgtccgt
     2161 ctgtatagat gcgcatgata cccgggccgt tctcggcaag gatgtagacg gcatctcgcg
     2221 tgacggcgaa ctcgtagacg tcgggcacgt tcatgatccg cgggaaacca tgcggaatac
     2281 cgcaagcgct gatcggatcc ggaaagttgc cctcgcgaac gagctcgatg ttgcgccggt
     2341 agatcgcttc ccactcttcg ttgtacggcg gaaactcgcg cacaccgggc tgccccgttc
     2401 ggccatcggc tggtcgaacc gtagagcggt cgaaaacagt gggcccttgc atcgcccaga
     2461 cgccgctcca atcgggaagc gctgctggcg cctggccgcg ggccgtttcg acggtcacgg
     2521 cggcgcctcc ggcgaaggcg acagccaacg cgccgagacg agcggtggat accagtcccc
     2581 tcatgcccac accccctgca aggtgccggt gttacggttt cggttcaacc gtaactccgg
     2641 cacttcggtt gaaccgaaac cgtaacaccg gcacctcgtc gaaaaaagga ggccgtcact
     2701 cgcgtgacgg gctcgggttt tctcgccggt ggactcgagc tattgcttga ggtcgagctt
     2761 gatgtcgacg gcgacgtctt gcgacaccca gctcgtgtcg ttccaacctt cgccgacgtt
     2821 gaagtcgaag cggttgat
//
LOCUS       DASSNN010000789         3995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_375404, whole genome shotgun sequence.
ACCESSION   DASSNN010000789 DASSNN010000000
VERSION     DASSNN010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375404"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..783
                     /locus_tag="VFL84_14840"
     CDS             130..783
                     /locus_tag="VFL84_14840"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET7609956.1"
                     /translation="MFSGLVKGVGRVASRTNTGADARFVIELGSLGPAPLAVGGSIAV
                     NGVCLTAIAVGHGGFTADVSAATLAVTTMGRLAAGARVNLEPPLKAGDPLDGHIVTGH
                     VDGVGEVISVETAGGSKRIVIAVPDALSRYIAPKGSIAVDGVSLTVNAVGATEFEVNI
                     IPHTQAATVIGEYARGTAVNIEVDLLARYLERLVRKDDRGGVDLAFLRKHGYARDHR"
     gene            797..1927
                     /gene="ribBA"
                     /locus_tag="VFL84_14845"
     CDS             797..1927
                     /gene="ribBA"
                     /locus_tag="VFL84_14845"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET7609957.1"
                     /translation="MNTTEEIVAAIRAGEMVVILDDEERENEGDLVMAATKVRAEDIN
                     FMAKYGRGLICLTLTRARCEQLRLPLMVSNTDVRQTTNFTISIEAAEGVTTGISAHDR
                     ARTIQAAVAASAQPSDLRQPGHIFPLMAQPGGVLTRAGHTEAGCDLARLAGLEPAAVI
                     VEILNDDGAMARRDDLVKFARRHELKIGTIADLIEYRLRTEESVEIIGETEVETEFGP
                     FRLVCFEDHVNRAVHLALARGKLDAKTPTLVRVHRQDTLSDVLGLHGPTLHRPLRDAL
                     RLISEADHGVAVILRLEETARDLMHSVQSLNVPPEPEVGAKQHRAMDFRMFGIGAQIV
                     RALGVRRMRVVGAPKQMHGLSGFGLEVVEYVSDAAERDDAHG"
     gene            1920..2399
                     /gene="ribE"
                     /locus_tag="VFL84_14850"
     CDS             1920..2399
                     /gene="ribE"
                     /locus_tag="VFL84_14850"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET7609958.1"
                     /translation="MDDRIKTLEGDFNARGLRFGIVASRFNDFIVDRLLDAAVATLLK
                     HGVAANDIQVVRVPGAFETPLAIKKMAAGRRYNALIALGCVIRGATPHFDHVAREASH
                     GIAEVSTNEEIPVGFGILTVDTIEQAIERAGTKAGNKGADAALAAIQMATLLRQMEQ"
     gene            2402..2848
                     /gene="nusB"
                     /locus_tag="VFL84_14855"
     CDS             2402..2848
                     /gene="nusB"
                     /locus_tag="VFL84_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011589448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET7609959.1"
                     /translation="MTQERGAGARHLARELLVKALYQWQMAGHSVAEISAQFAAHEDF
                     ERSDRDFFGQLLAVTIEDAPALDAIIARQAARGLEQLDAVARAILLLGLAELKFRDDV
                     PSKVVINEAVELAKRYGPADSFKFVNAVLDKSSRELRGAAKRGGET"
     gene            2848..3798
                     /gene="thiL"
                     /locus_tag="VFL84_14860"
     CDS             2848..3798
                     /gene="thiL"
                     /locus_tag="VFL84_14860"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016192778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET7609960.1"
                     /translation="MGEFEVIARYFTRASADRDITLGIGDDAAVLAVAGHVAVTVDTL
                     VAGVHFPDGMAPHLLGYRLLAVNLSDLAAMGARPRWGTLALTMPSADELWLDGFSRGL
                     FELADRYGVSLVGGNLSRGPLTLTMQLLGTVEPRNMLTRGGGHVGDDVYVTGTLGDSS
                     AGIMLILERSAAPQGSAAGALKERFYRPVPRVGAGLALGPLATAAIDVSDGLLADLGH
                     ICKASGCGAAIDIERVPLSAELLSLFPPQEALAHALGGGDDYELCFTAPPSRAEEIEA
                     ALEAAATPVRRIGQLAAGQTVVCRRDGEPFAPTVRGYRHF"
BASE COUNT          614 a         1343 c         1409 g          629 t
ORIGIN      
        1 gccggcgctc gcatcgttgg ccgacgcaac cgaattgacg ttcgacggcg tcagcatggt
       61 cggggccgac ttgcggatca ttgcgcgcgt cagtagggtg gctcgcgcct ccggccgcta
      121 ggagaacgag tgttctcggg attggtgaaa ggagtcggtc gagtcgcgtc gcgcacgaac
      181 accggcgcgg atgcgcggtt cgtgatcgag ctcggcagct tgggtccggc accgctcgcg
      241 gtcggcggca gcatcgccgt gaacggcgtc tgccttacgg cgatcgcggt cgggcacggc
      301 ggcttcacgg ccgatgtgtc ggccgcgact ctggccgtca cgacgatggg ccggctcgcg
      361 gcgggcgccc gcgtgaatct cgagccgcct ctcaaggccg gcgatccgct cgacgggcac
      421 atcgtgacgg gtcacgtcga cggcgtcggc gaggtcattt ccgtcgaaac cgccggcggc
      481 tcgaagcgca tcgtcatcgc ggttccggac gcgctctcaa ggtacatagc gccaaaggga
      541 tcgatcgccg tcgacggggt cagtctcacg gtcaatgccg tcggcgcgac ggaattcgag
      601 gtcaatatca tcccgcacac gcaagccgcg accgtgatcg gcgagtacgc gcgcggcacg
      661 gctgttaata ttgaggtcga cctgctggcg cggtatctcg agcgcctcgt tcgcaaagac
      721 gaccgcggcg gcgtggatct cgcctttttg aggaaacatg gctacgcacg cgaccaccga
      781 taaagcctgg tcggagatga acacgaccga ggagatcgtc gccgcgattc gcgcgggcga
      841 gatggtcgtc attctcgacg acgaggagcg cgagaacgag ggcgacctcg tcatggcggc
      901 gacgaaagtg cgcgccgaag acatcaactt catggcgaag tacggccgcg gcttgatctg
      961 cctcacgctg acgcgcgcgc gttgcgagca gctgcgcttg ccgctcatgg tcagcaatac
     1021 cgacgtgcgg cagacgacga acttcacgat ctcgatcgaa gcggcggagg gcgtgacgac
     1081 gggcatctcc gcgcacgacc gcgcgcgcac gattcaagcc gccgttgccg cgtcggcgca
     1141 gccgagcgat ctgcgccagc ccggccatat ctttccgttg atggcgcagc ccggcggcgt
     1201 gctgacgcgc gcgggccaca ccgaggccgg ctgcgatctc gcgcgcctcg ccggcctcga
     1261 gcccgcggcc gtcatcgtcg agatcctgaa cgacgacggc gcgatggcgc ggcgcgacga
     1321 tctcgtcaag ttcgcgcgcc gtcacgagct caagatcggc acgatcgcgg atctcatcga
     1381 gtatcgcctg cgaaccgagg agtccgtcga gatcatcggc gagaccgagg tcgagaccga
     1441 gttcggcccg ttccgtctcg tctgcttcga ggatcacgtc aaccgcgccg tgcacctcgc
     1501 gctcgcgcgc ggcaagctcg atgcgaagac gccgacgctc gtgcgcgtcc accggcagga
     1561 cacgttgagc gacgtgctcg gcctgcacgg tccgacgttg caccgcccgc tgcgcgacgc
     1621 gctgaggctg atcagcgagg cggatcacgg agtcgcggtc atcctgcggc tcgaagaaac
     1681 cgcgcgcgat ctcatgcact cggtgcagtc gctgaacgtg ccgcccgagc ccgaggtcgg
     1741 cgccaagcag catcgcgcca tggatttccg catgttcggc atcggcgcgc agatcgtgcg
     1801 tgcgctcggc gtgcgccgca tgcgcgtcgt cggcgcgccg aagcagatgc acggcctgtc
     1861 cggtttcggg ctcgaggtcg tcgagtacgt ctccgacgcc gcggaacggg atgacgcgca
     1921 tggatgaccg catcaagacg ctcgaaggcg atttcaacgc gcggggcctg cgcttcggca
     1981 tcgtcgcctc gaggttcaac gacttcatcg tcgatcgcct gctcgacgcc gccgtcgcca
     2041 cgctcttgaa gcacggcgtc gcggcgaacg acattcaggt cgttcgagtg ccgggcgcgt
     2101 tcgagacgcc gctcgcgatc aagaagatgg ccgcggggcg ccgctacaac gcgctgatcg
     2161 cattggggtg tgtgatccgc ggcgccacgc cgcacttcga ccacgtcgcg cgcgaggcga
     2221 gtcacggcat cgccgaggtc agcacgaacg aggaaattcc cgtcggcttc ggcatcttga
     2281 ccgtcgacac gatcgagcag gccatcgagc gcgcgggaac gaaagccggc aacaagggcg
     2341 ccgatgcggc gctcgccgct attcagatgg ccacgttgct gcgccagatg gagcagtagg
     2401 cttgacgcag gagcgcggtg cgggggcgcg gcacctggcc cgagagctgc tcgtcaaagc
     2461 gctctatcag tggcagatgg cgggccacag cgtcgccgag atctcggcgc aattcgccgc
     2521 gcacgaggac ttcgagcgca gcgaccgcga tttcttcggc cagctgctcg ccgtgacgat
     2581 cgaggatgcg cccgctctcg acgcgatcat cgcgcgccaa gcggcgcgcg gcctcgagca
     2641 gctcgacgcg gtcgcgcgag cgatcctgct gctcgggttg gccgagctca aatttcgcga
     2701 cgacgtgccg agcaaggtcg tcatcaacga ggccgtcgag ctcgcgaaac gctatggtcc
     2761 ggccgacagc ttcaagttcg tgaacgcggt tctcgacaag agctcgcgcg agctgcgcgg
     2821 agcggcgaag cgcgggggcg agacttgatg ggcgagttcg aggtcatcgc gcgctacttc
     2881 acgcgcgctt ccgccgatcg cgacatcacg ctcgggatcg gcgacgacgc ggccgttctc
     2941 gcggtcgccg ggcacgtggc ggtaaccgtc gacacgctcg tcgcgggcgt gcatttcccc
     3001 gacggcatgg ctccgcatct gctcggctac cgcttgctgg ccgtgaactt gagcgacctg
     3061 gccgcgatgg gcgctcggcc gcgctggggc acgctcgccc tgacgatgcc gtcggccgac
     3121 gagctctggc tcgacggttt cagccgcggc ttgttcgagc tcgcggaccg ctacggcgtg
     3181 agcctcgtcg gcggcaactt gagccgcggg ccgctgacgt tgacgatgca gctgctgggt
     3241 accgtcgagc cgagaaacat gctcacgcgc ggcggcggcc acgtcggcga cgacgtctac
     3301 gtgaccggga cgctcggcga cagctcggcg ggcatcatgc tgattctcga gcgcagcgcg
     3361 gcgccgcaag gctcggcagc cggggctctc aaggagcgct tctatcgtcc ggtgccgcgc
     3421 gtcggcgcgg gcttggcgct cgggccgctc gcgacggcgg ccatcgacgt gtcggacggg
     3481 ttgctcgccg acctcggcca catctgcaag gcgagcggct gcggcgccgc gatcgacatc
     3541 gagcgcgtgc cgttgtcggc cgagctcttg tcgttgtttc cgccgcagga agcgctcgcg
     3601 cacgcgctcg gcggcggcga cgactacgag ctgtgcttca ccgcgccgcc gtcgcgcgcc
     3661 gaggagatcg aagcggcgct cgaggccgcc gccacgcccg tgcgccgcat cggtcagctc
     3721 gccgcgggcc agaccgtcgt atgccggcgc gacggcgagc cgttcgctcc gacggttcgc
     3781 gggtataggc atttttgacc caagcggggc gcccgcgggt tgcgcgatgc aggagccatt
     3841 gccgagccgg ccgacgagcg tggggacgct gcgcgatccc gtccacttca tcgcattcgg
     3901 tttcggcgcc gggctcgcgc cgaaggcgcc cggcacgttc ggcagcgtcg tcggcgccgt
     3961 cgcggcctgg tggctgctcg agctcccgct cgccg
//
LOCUS       DASSNN010000790         4283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1723731, whole genome shotgun sequence.
ACCESSION   DASSNN010000790 DASSNN010000000
VERSION     DASSNN010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723731"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1273)
                     /locus_tag="VFL84_14865"
     CDS             complement(125..1273)
                     /locus_tag="VFL84_14865"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET7609961.1"
                     /translation="MLGDLIAAGRGHSSKSMRLMHTLAAQLTVALVASLAVASSAAQP
                     ANPAPRPFRVVRADPKLDAVVSSDARLELIGDRFGLTEGPVWVPDGGDGYLLFSDLIS
                     NVIYRWAPGEPISVHLDRAGYSGNELTKAGFQTRRGRMAVLLVGPNGLSLDAEGRLLY
                     LAANDRAVMRLERDGKRTVVADRYDDRRFNGPNDLAVHSNGAIYMTDSVWGLRDGLAN
                     PDSQLNFSGVYLIRGGKTTLLYSDADNPGGWPNGIALSPDERYLYMNTGVQNVLRYEL
                     APDGTLRNRTVFIAGEGSDGMKVDTLGNLYTTNGAGAGEVRITSPEGVRLGVLELPVP
                     AGEPQAQVCATNVAFGDRDSRTLYITACEHVYRIRMNVAGVHPAAARH"
     gene            complement(1258..2055)
                     /locus_tag="VFL84_14870"
     CDS             complement(1258..2055)
                     /locus_tag="VFL84_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609962.1"
                     /translation="MNRRLRWVLACGFGLAASTALLGTADAQQRRAAPAAPPASAKAG
                     APIDITGHWVSLITDDWVYRMITPAKGDVSYVPLNAEGRRVANAWDPARDEAAGEQCK
                     GYAAPAIMRLPSRVEITWQDDNTLKLDIDTGMQTRLFHFGQPEPQGARSWQGWSAASW
                     QLSGTTEQFYPGPTSLGQIKRAGSLKVDTSNLRAGYLRKNGVPFSENAFMTEYYNLIV
                     EDDGNQYLVIQTFVSDPRYLTNHFVRTMQFKREKDGSKRNPVPCSAT"
     gene            complement(2089..2484)
                     /locus_tag="VFL84_14875"
     CDS             complement(2089..2484)
                     /locus_tag="VFL84_14875"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609963.1"
                     /translation="MKWKLVIFAAAGTLLSLSSASAHHSFTATYDEAKTIQIEGELVQ
                     FMFRNPHAWVHVMAPDENGTMQRWGVEWGGAGALTGQGVTRDSLKPGDKVIITGNPGR
                     NPIDHRLRMRTLLRPSDGFGWGQRGETFD"
     gene            complement(2504..3634)
                     /locus_tag="VFL84_14880"
     CDS             complement(2504..3634)
                     /locus_tag="VFL84_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609964.1"
                     /translation="MTAASSSHALPRRFAAALMLSISLLALAAPQAHAQQQQRSAQQR
                     PVQRTGESAGDLHWWPVRKNIWMLVGAGTNIAASVGPDGVLLVNAGTADKSARVIDAI
                     KELQGQLNAFGFLDVTQPPRGGAETRSRFPVNTHPPPKPVRYIVNTSSLPHSIGGNEA
                     LAASGVTYTGGNVAGTIADSSEGAAVLAHENVLNRMVARDLPFGALPTETFFTAEYKV
                     STFFNDEGIQIVHVPNAATDGDSFVFFRGSDVIAAGDLVNMDTFPIIDVESGGSINGV
                     LAGLNAILKLAIPEFRTEGGTMIVPGHGRLADSADVGYYRDMLTIIRDQVQALIDEGL
                     SLEQVIERNPTFGYDGRFGSTTGPWTTNMFIEAVYTSLKKKS"
     gene            complement(3634..>4283)
                     /locus_tag="VFL84_14885"
     CDS             complement(3634..>4283)
                     /locus_tag="VFL84_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609965.1"
                     /translation="RPHSAAYIKRGPSNLRVWKEVDPASRQIIAFQTEWLRSTNTPIY
                     MDGRPHPPEYAAHTWGGFSTGTWEGDRLRVVTTHMKEDYYRRNGVPQSDTARLTEYLI
                     RRRFQNEDYLTWVIVAYDSVYLTEPLIRSTEYRLNLAQQVPPYPCTIVTEVVRPEGYV
                     PSWLPGKNDQIYSFSDFYGLPRELYFDGAETMYPEYHKKLERLGATAAKQAPGRD"
BASE COUNT          673 a         1400 c         1437 g          773 t
ORIGIN      
        1 gccgctcgtc gagcggaaag ccggtgccct tgttcagcgc gggattacga atcagcgccg
       61 agccgcgacc cgtgaccttc aggttgcgag gtgacgacct tctgtcgttg cggaccgcca
      121 tcgttcagtg cctcgccgcg gccgggtgaa ccccggcgac attcatgcgg atgcggtaca
      181 cgtgctcgca tgcggtgatg tagagcgtgc ggctgtcgcg atcgccgaac gcgacgttcg
      241 tggcgcaaac ctgtgcttgc ggctcgccgg ccggcacggg cagctcgagc acgccgagcc
      301 gcacgccttc cggcgaggtg atgcgcactt cgccggcgcc tgcgccgttc gtcgtgtaca
      361 ggttgccgag cgtgtcgacc ttcatgccgt cgctgccttc gccggcgatg aataccgtgc
      421 gattgcgcag cgtgccgtcg ggggcgagct cgtagcgcag cacgttttga acgcccgtat
      481 tcatatatag atagcgctcg tcgggcgaca gcgcgatgcc gttgggccag ccgcccggat
      541 tgtcggcgtc gctgtacagc agtgtagtct tgccgccgcg gatcagataa acgccgctga
      601 aattcagctg cgaatcggga tttgcgagcc cgtcgcgcaa gccccagaca ctgtcggtca
      661 tgtagatcgc gccgttcgag tgcacggcga gatcgttcgg gccgttgaat ctgcggtcgt
      721 cgtagcggtc ggcgacgacc gtccgcttgc cgtcgcgctc gagccgcatc acggcgcggt
      781 cgttcgcggc gagatagagc aagcgtcctt ccgcgtcgag cgacaaaccg ttcgggccga
      841 ccagcaggac ggccatgcgc ccgcgccgcg tctgaaagcc cgctttcgtg agctcgttgc
      901 cgctgtagcc ggcgcgatcg aggtgcacgc tgatcggctc gccgggcgcc cagcgataga
      961 tcacgttcga gatcaaatcg ctgaacaaga ggtatccgtc accgccgtcg ggcacccaga
     1021 ccgggccttc cgtgaggccg aagcggtcgc cgatgagctc gaggcgcgcg tcgctcgaga
     1081 cgaccgcgtc gagcttcggg tcggcgcgca cgacccgaaa cggccgcggc gccggattcg
     1141 ccggctgcgc cgccgacgac gcgacggcga gcgacgcaac caatgcgacc gtcaactgcg
     1201 ccgcgagcgt gtgcatgagc cgcatcgatt tcgaggagtg accgcgtccc gctgcgatca
     1261 ggtcgccgag cacggcacgg gattgcgctt gctgccgtct ttctcgcgtt tgaattgcat
     1321 cgtgcgcacg aagtgattcg tcaggtagcg cggatccgag acgaaggtct ggatgacgag
     1381 gtactgattg ccatcgtcct cgacgatgag gttgtagtac tccgtcatga acgcgttctc
     1441 gctgaaaggc acgccgttct tgcgcaggta ccccgcccgc agattcgacg tgtcgacttt
     1501 gaggctgccc gcgcgcttga tctggccgag cgacgttggg ccgggataga actgctcggt
     1561 cgtgcccgac agctgccacg acgcggcgga ccagccttgc cagctgcgcg cgccttgcgg
     1621 ttcgggttgg ccgaagtgga acaggcgcgt ttgcatcccg gtgtcgatgt cgagcttgag
     1681 cgtgttgtcg tcttgccacg tgatctcgac acggctcggc agccgcatga tcgcgggcgc
     1741 cgcgtatccc ttgcattgct cgcctgccgc ctcgtcgcgg gccggatccc acgcattggc
     1801 gacgcggcgg ccttccgcgt tcagcggaac gtacgacacg tcgcccttcg ccggcgtgat
     1861 catgcggtag acccagtcat ccgtgatcag cgagacccag tggcccgtga tgtcgatcgg
     1921 cgccccggct ttcgccgagg cgggaggcgc ggccggtgcg gcacggcgct gctgcgcgtc
     1981 ggccgtgccg agcaacgccg tgctcgcggc caagccgaag ccgcaagcga gcacccatcg
     2041 cagtctacgg ttcatgtcga tctccgccga ctcgagacgc gcgccgcatc aatcgaacgt
     2101 ctcgccgcgc tggccccagc cgaagccgtc ggacggccgc agcagcgtgc gcatgcgcag
     2161 ccggtgatcg atcggattgc ggcccggatt gcccgtgatg atgaccttgt cgcccggttt
     2221 cagcgagtcg cgcgtgacgc cttgacccgt cagcgcgccc gcgccgcccc attcgacgcc
     2281 ccagcgctgc atcgtgccgt tctcgtcggg cgccatgacg tgcacccacg cgtgcgggtt
     2341 gcggaacatg aactgcacga gctcgccttc gatctggatc gtcttggctt cgtcgtacgt
     2401 cgccgtgaac gagtgatgcg ccgaggcgct cgagagcgac agcaaggtgc cggcggctgc
     2461 gaagatgacc aatttccatt tcacgtgcat gacctccgaa tattcaagac ttcttcttca
     2521 agctcgtgta gacggcctcg atgaacatgt tcgtcgtcca cggtcccgtc gtcgagccga
     2581 agcgcccgtc gtagccgaac gtcggattgc gctcgatgac ttgctcgagc gagagaccct
     2641 cgtcgatcag cgcttggacc tgatcgcgaa tgatcgtcag catgtcgcgg tagtagccga
     2701 catcggccga gtccgcgagc ctgccgtggc cgggcacgat catcgtgccg ccttccgtgc
     2761 ggaactccgg gatcgcgagc ttcaagatcg cgttcaagcc cgcgagcacg ccgttgatgc
     2821 tgccgccgga ctcgacgtcg atgatcggaa acgtatccat gttgacgaga tcgcctgctg
     2881 cgatgacgtc ggagccgcgg aagaacacga agctgtcgcc gtcggtcgcc gcgttcggga
     2941 cgtggacgat ttgtatgccc tcgtcgttga agaacgtgct gaccttgtac tcggccgtga
     3001 aaaacgtctc ggtcggcagc gcgccgaacg gcaggtcgcg cgcaaccatg cgattcagca
     3061 cgttctcgtg agcgagcacc gcggcgcctt cggacgagtc ggcgatcgtg cctgcaacgt
     3121 tgccgcccgt ataggtaacg ccgctcgccg cgagcgcctc gttgccgccg atgctgtgcg
     3181 gcagcgagct cgtgttgacg atgtagcgga ccggcttcgg cggagggtga gtgttgacgg
     3241 gaaagcgcga gcgcgtctcg gccccgccgc gcggcggctg cgtgacatcg aggaagccaa
     3301 acgcgttcag ttgcccttgc agctccttga tcgcgtcgat gacgcgcgcg ctcttgtccg
     3361 cggtgccggc gttgacgagc agcacgccgt cgggcccgac cgacgcggcg atgttcgtgc
     3421 ccgcgccgac gagcatccag atgttcttgc gcaccggcca ccagtgcagg tcgccggcgc
     3481 tctcgccggt gcgctgcacg gggcgctgct gcgcggagcg ctgctgttgc tgcgcgtgcg
     3541 cttgcggcgc cgccaacgcg agcagcgaga tcgacaacat gagcgccgcg gcgaagcggc
     3601 gcggcagcgc gtgcgaagag gaagcggccg tcatcagtct cgtcccggag cctgctttgc
     3661 ggccgtcgcc ccgagacgct cgagcttctt gtgatactcc ggatacatcg tctcggcgcc
     3721 gtcgaagtag agctcgcgcg gcagcccgta gaaatcggag aagctgtaga tctgatcgtt
     3781 cttaccgggc agccagctcg gcacgtagcc ctcggggcgc acgacctcgg tgacgatcgt
     3841 gcaagggtag ggcggcactt gctgcgccaa gttcagccga tactcggtgc tgcggatcaa
     3901 cggctcggtg agatagacgg agtcgtacgc gacgatgacc cacgtcaggt aatcctcgtt
     3961 ctgaaagcgc cgccggatca gatactccgt gagccgtgcc gtgtcgctct gcggcacgcc
     4021 gttgcggcgg tagtagtcct ctttcatgtg cgtcgtcacg acgcgcaggc gatcgccttc
     4081 ccaggtgccc gtcgaaaagc cgccccacgt atgggccgcg tactcgggcg gatgcgggcg
     4141 cccatccatg taaataggcg tgttcgtcga gcgcagccac tcggtctgga acgcgatgat
     4201 ttgccgcgac gccggatcga cctctttcca cacgcgcaga ttcgacgggc cgcgcttgat
     4261 gtaggccgcc gagtgcggcc ggc
//
LOCUS       DASSNN010000791         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1343582, whole genome shotgun sequence.
ACCESSION   DASSNN010000791 DASSNN010000000
VERSION     DASSNN010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343582"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFL84_14890"
     CDS             complement(<1..298)
                     /locus_tag="VFL84_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609966.1"
                     /translation="MRITRFVRSALLTSAAALFVLTLVPAEAQRDTDRFVDAAKVLEL
                     FTTDDEHGIPADILQRARGIAVIPNLIRGGLLFGGRRGRGVLAIRSPNGEWSNPA"
     gene            complement(376..>2138)
                     /locus_tag="VFL84_14895"
     CDS             complement(376..>2138)
                     /locus_tag="VFL84_14895"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET7609967.1"
                     /translation="QWVGGHAGLVLGIAAGSFGLMVLASFFRTAQLSRGGGHVAQSLG
                     GTRVTGDGNDPLERRLVNVVEEMALASGLPVPEIYVLQQEAAINAFAAGRTGSDAAIA
                     VTRGALERLTRSELQGVIAHEFSHILNGDMRLNQQLIGLSFGILVLSLIGRWLLRSMR
                     YSRPRRGRGGGGIAAAVAIAVALLVIGWVGVLLSRLIKAGVSRQRERLADASAVQFTR
                     EPEGLAGALKKIGGYSAKIVSVDTEEVSHMLFEGRSSAFSGWFATHPPLLERIRALEP
                     GFDPRDLPTSVEPLPPPGTAEPAEGGAAGLVAAGPAGASPLERAGEIAAPAGRALHGA
                     VPDEVLHAARSREGSLLLVVALALSSDAATRRKQLAFVEQRLGTQRTELCKRLLGELE
                     RAPAELRLPVLELALPSLRQRPRGELAYVVELTTRLQELETAPSLFNFVLLRLVKAFF
                     RDVPGVDAAPRRADRALDPRAAVRTLLATVAAFGSERATDARAAYAAGIESVGWRMEP
                     QDPTFEPPAALRNLERLDSALAALAAIRPREKQRVLRGVLAAIRADAVTAAEERELFR
                     VIAVALDCPVPPDVAATVAK"
BASE COUNT          324 a          760 c          787 g          267 t
ORIGIN      
        1 acgccgggtt gctccattcg ccgttcggcg agcgaatcgc gagaacgccg cggccgcggc
       61 gcccgccgaa cagcagaccg ccgcggatca agttcgggat gacggctatt ccgcgcgcgc
      121 gctgcaagat atcggcggga attccgtgct cgtcgtccgt cgtaaagagc tcgagcacct
      181 tggcggcgtc gacgaaccgg tccgtgtcgc gctgcgcctc ggcgggcacg agcgtgagca
      241 cgaacagcgc ggcggccgac gtcaggagtg ccgagcgaac gaagcgggtg atgcgcatgg
      301 agccctcgtg gacgcgactc cgcgcatctt agcgaaatac ggctgaggtg ccagccgcca
      361 tttcggcggt tgccgctatt tggcgacagt ggcggcgacg tcgggtggca cggggcagtc
      421 gagcgcgacc gcgatcacgc gaaagagctc gcgctcctcg gccgcggtga cggcatcggc
      481 gcgaattgcg gccaagacgc cgcgcagcac gcgctgcttc tcgcgcgggc gaatcgccgc
      541 gagcgccgcg agcgccgaat cgagccgctc gaggttccgc agcgccgccg gcggctcgaa
      601 cgtcgggtct tggggctcca tgcgccagcc caccgattcg atgccggccg cataggccgc
      661 gcgggcgtcg gtcgctcgct cgctgccgaa agccgcgacc gtggcgagca gggtgcgcac
      721 ggcggcgcgc gggtcgagcg cgcggtcggc acgccgcggg gccgcatcga cgccgggcac
      781 gtcgcggaag aacgctttga ccaaccgcag cagcacgaag ttgaacaggc tcggtgcggt
      841 ctcgagctcc tgcaggcgcg tcgtgagctc gacgacatag gcgagctcgc cgcgcggccg
      901 ctggcgcaag gaaggcagcg cgagctcgag cacgggcaag cgaagctcgg ccggcgcccg
      961 ctcgagctcg ccgagcaacc gcttgcagag ctcggtgcgc tgcgtgccga gccgttgctc
     1021 gacgaaggcg agctgcttgc ggcgagtcgc cgcgtcgctc gacagtgcca gcgcaacgac
     1081 gaggagcagg ctcccttcgc gcgagcgcgc ggcatgcagc acctcgtccg gcaccgcgcc
     1141 gtgcagcgct cggccggccg gcgcggcgat ctcgcccgcg cgctccagag gcgacgcgcc
     1201 cgcggggccg gccgcgacga gcccggccgc gccgccttcg gccggctcgg cggtcccggg
     1261 cggcggcagc ggctcaacgg aggtcggcaa gtcgcgcggg tcgaagcccg gctcgagcgc
     1321 gcgaatgcgc tcgaggagcg gtgggtgcgt cgcaaaccag ccgctgaacg cgctcgagcg
     1381 gccttcgaac agcatgtgcg agacctcctc ggtgtcgacc gagacgatct tcgccgagta
     1441 gccgccgatc ttcttcaatg cgcccgcgag cccttcgggc tcgcgcgtga actgcacggc
     1501 cgaggcgtcg gccaatcgct cgcgctgccg cgacacgccg gccttgatga gccgcgagag
     1561 cagcacgccg acccagccga tgacgagcag cgcgacggcg atcgccacgg cggccgcgat
     1621 gccgccgccg cctcggccgc ggcgcgggcg cgagtagcgc atgctgcgca gcagccagcg
     1681 cccgatcagc gacaggacga gaatgccgaa cgacagaccg atcaattgct ggttcagccg
     1741 catgtcgccg ttcaagatgt ggctgaactc gtgcgcgatg acgccttgca gctcgctgcg
     1801 cgtgagccgc tcgagcgcgc cgcgcgtcac ggcgatcgcg gcgtcgctgc ccgtgcgtcc
     1861 cgcggcgaag gcgttgatcg ccgcttcctg ctgcaggacg tagatctccg gcaccggcaa
     1921 ccccgaggcg agcgccatct cctcgacgac gttgacgagg cggcgctcga gcgggtcgtt
     1981 gccgtcgccg gtgacgcgcg tgccgccgag cgactgcgcg acgtggccgc cgccgcgcga
     2041 gagctgcgcg gtccgaaaga agctcgccaa gaccatgagg ccgaacgatc ccgccgcgat
     2101 tccgagcacg agaccggcgt ggccgccgac ccattggc
//
LOCUS       DASSNN010000792         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_709561, whole genome shotgun sequence.
ACCESSION   DASSNN010000792 DASSNN010000000
VERSION     DASSNN010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709561"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /locus_tag="VFL84_14900"
     CDS             complement(<1..1745)
                     /locus_tag="VFL84_14900"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7609968.1"
                     /translation="MRKSLCLAIGLGISAGTVAQPSPPVEVIEIVGVTPLGGDLDTNR
                     IAANVQTASAAELREKNALDLTDFMKRSMGSVFVNDAQSNPLQPDVQYRGFVGSPLLG
                     LPQGLAVYQDGVRVNEPFGDTVSWALIPESAIKELYLVPGSNPLFGLNALGGAIAVRT
                     KDGFANDDTSAELTAGSFGRVSVQAETGGAFSDDLGYFLTVSLLEEDGWRDYSPTDAQ
                     QIFGKLTWRGDNSRLDVSLTRADTDLVGNGAAPEDLLEMDPEAIFTHPDHTENALTML
                     GVTAEQSLSPNVTLRGNIYVRASDIDTLNGDDSDFQECTATPGFICEDEDGTEEVALD
                     ENGLPIAADDDLEGGSVNRTDTEQDGKGFGLQADFTGDLAGRDNLFTIGVALDEGDIE
                     FGASTELGALDSTRQAIGGGVFVGEAFTGLESSISNTGFYLSNTFSLGERTALTVSGR
                     YNRTHVELEDQIEDDLDGDHTFERFNPAIGVTFGTEAVTFYAGYSEANRAPSPVELTC
                     ADEDDPCRLPNAFLADPPLEQVVARTLEAGFRGDLKGGSWHAGVFRTTNDDDILFISA
                     GALTNQGFFDNVGETR"
     gene            1818..>2261
                     /locus_tag="VFL84_14905"
     CDS             1818..>2261
                     /locus_tag="VFL84_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7609969.1"
                     /translation="MTIPATMTAMRLKGPGRALERDVVPVPQPSPTEVLLEVLACGVC
                     RTDLHVLDGELPNLRYPVTPGHEIVGRVAAVGPAVENVKVGERVGVPWLVRTCGQCEP
                     CRRGLENLCERAEFTGYTVPGGYAEYVTADARYCLHVPERYSDSAA"
BASE COUNT          351 a          723 c          753 g          434 t
ORIGIN      
        1 cgcgtctcgc cgacgttgtc gaaaaaccct tggttcgtga gcgcccccgc gctgatgaac
       61 aagatgtcgt cgtcgttcgt ggtgcggaaa acgcctgcgt gccagctgcc gcctttcaag
      121 tcgccgcgaa agccggcctc cagcgttcgt gcgacgactt gctcgagcgg cggatccgcg
      181 aggaaggcgt tcggcaaacg gcacgggtcg tcctcgtcgg cgcaggtcaa ttcgaccggt
      241 gaaggtgcgc ggttggcctc gctgtagccc gcgtagaacg tcacagcttc cgtgccgaag
      301 gtgacaccaa tggcgggatt gaagcgctcg aacgtgtggt cgccgtcgag gtcgtcttcg
      361 atttggtcct cgagctcgac gtgcgtgcgg ttatagcgcc cggacacagt gagcgccgtg
      421 cgctcgccga gcgagaatgt gttgctcagg tagaagccgg tgttcgatat ggacgattcg
      481 agccccgtga aggcctcacc gacgaacacg ccgccgccga tggcttgccg cgtggagtcg
      541 agcgccccga gctccgtgct cgcgccgaac tcgatgtcgc cttcgtcgag tgccacgccg
      601 atcgtgaaga ggttgtcgcg gccggcgaga tcgcccgtga aatcggcctg caacccgaac
      661 cccttgccat cttgctcggt gtcggtgcgg ttgacgctcc ctccctcgag gtcgtcgtcg
      721 gctgcgatcg gtaggccgtt ctcgtcgagc gcgacctcct ctgttccgtc ctcgtcctcg
      781 cagatgaagc ctggcgtggc cgtgcattct tggaagtccg agtcgtcgcc gttcagggtg
      841 tcgatgtcgc tcgctcttac atagatgttg ccgcgcagag tgacgttcgg cgacagcgat
      901 tgctcggcag tgacaccgag catcgtcaac gcattctcgg tatggtcggg atgcgtgaag
      961 attgcttcgg gatccatctc gagcaaatcc tcgggcgccg cgccgttgcc gaccagatcg
     1021 gtgtcggcac gcgtcaggct aacgtcgagg cgcgagttgt cgccacgcca cgtcagcttg
     1081 ccgaagatct gctgcgcgtc ggtcggcgaa taatcgcgcc aaccgtcctc ttcgaggagc
     1141 gacaccgtga ggaaatagcc taggtcgtcg ctgaaagcgc caccggtctc ggcctggacg
     1201 ctcacgcggc cgaacgatcc cgcggtcagc tccgcgctcg tgtcgtcgtt ggcgaagccg
     1261 tccttggttc gtaccgcgat cgcgccaccg agcgcgttca agccgaacag cgggttggat
     1321 cccggaacga gataaagctc tttgatcgcg ctttctggaa ttagcgccca gctcacggtg
     1381 tcgccgaacg gctcgttgac gcgtacgccg tcctggtaga ccgcgagccc ttgcggcagc
     1441 ccgagcagcg gcgagccgac gaagccgcgg tattggacgt ccggttgcag cgggttgctc
     1501 tgcgcgtcgt tgacgaacac gctgcccatg ctgcgtttca tgaagtctgt caaatcgagc
     1561 gcgtttttct cgcgcagctc ggccgcgctc gcggtctgga cgttcgccgc aatgcggttg
     1621 gtatcgagat cgccgccgag cggcgtgacg ccgacgatct cgatgacttc aacggggggt
     1681 gacggctgcg cgaccgtgcc cgccgaaatg ccgaggccga tcgccaaaca caatgacttc
     1741 cgcaaatgct gtcctctaga gtcgcgcggc attgtaaccg agttgactgg gtctttcggg
     1801 gcgccgacgc tgatctcatg acgattcccg cgacgatgac ggcgatgcga ttgaagggtc
     1861 cgggccgtgc gctcgagcgc gacgtcgtgc ccgtgccgca gccgtcgccg accgaagtgc
     1921 tgctcgaggt gctcgcgtgc ggcgtctgcc gcacggactt gcacgtgctc gacggcgagc
     1981 tgcccaactt gcgttatccc gtgacgccgg gccacgagat cgtcggccgc gtcgccgccg
     2041 tcggcccggc agtcgagaac gtgaaagttg gcgagcgcgt cggcgtgccg tggctcgtcc
     2101 gcacgtgcgg ccaatgcgag ccgtgccgcc gcgggctcga gaacctctgc gagcgggccg
     2161 agttcaccgg ttatacggtg cccggcggct acgccgagta cgtgacggcc gacgcgcgtt
     2221 attgcctgca tgtgccggag cgctattcgg attccgccgc c
//
LOCUS       DASSNN010000793         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_710095, whole genome shotgun sequence.
ACCESSION   DASSNN010000793 DASSNN010000000
VERSION     DASSNN010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710095"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..412
                     /locus_tag="VFL84_14910"
     CDS             209..412
                     /locus_tag="VFL84_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609970.1"
                     /translation="MPGTQPHTQDGFTQTAAEPRVRKDAGDEGPEHDDPTTLKFEGTV
                     AISDEDAGSDPYNRTGRFRRAVR"
     gene            complement(421..2037)
                     /locus_tag="VFL84_14915"
     CDS             complement(421..2037)
                     /locus_tag="VFL84_14915"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HET7609971.1"
                     /translation="MKKVNAAGRAGLLAAAGLILCGVANAQLREPIKLDAGSLGNSTE
                     SSPGVRVFKGIPFAAPPVGALRWREPQPVAKWNGVRDASKFGNVCLQPDGGARGNIAV
                     GPGSPPQSEDCLYLNVWTGAQSATERRPVMVYFFGGAFSEGAGSIPLYDGDALAKKGA
                     VVVTMNYRLGPFGFFAHPALTADSPHKSSGNYGLADMVASLNWVKSNIAAFGGDPGNV
                     TVFGQSAGAMAIGALTSSPVTKGLYQRAIGQSGSWMGLGPSAAFATRARAESAGKGLA
                     DAAGVTTVEQLRAMPAADVTAKLSGRGIGGGGMIVDGWIIPEDPSDVFAAGKQNPVDV
                     LVGSNRDDLSFGPPRPVTPEQFEQGAAPRWGALADEFLTLYPHATNEEATKSQADSAN
                     DGAFWHMRLFADNQKKLGRQAWLFYFAQNPPGPPGQPAVPAAHASELPYVFNNLGKAP
                     LFPDPSDPALAAASAPDRKVADQMSSYWVNFARTGNPNGPKLPTWQEHRVGESDRAII
                     LDADPSSEHLPAKARLELYDKLYAQMRAAR"
BASE COUNT          329 a          725 c          737 g          335 t
ORIGIN      
        1 atgacggagc tcatcaactc gttgtcgcgc tagggccgcg attgctcggt tcgcaagcga
       61 tccggcgacg aacggtagcg tgccgccgat gagcggcgcc gagccgaccg gcggtagccg
      121 cggaccgacg gccgaccttc aggctgcggc cgtctctgcc tagactgcgt tgcaacgggc
      181 agcgcgcgcg ctgcggcgga ggacggcgat gccaggcacg caaccccaca cgcaagacgg
      241 ctttacgcaa acggccgcgg agccgcgcgt tcgcaaggat gccggtgacg aaggcccaga
      301 gcacgacgac ccgacgacgc tgaagttcga gggcacggtc gcgatctcgg acgaggacgc
      361 gggcagcgac ccctacaatc ggaccggccg cttccgtcgc gccgtgcgct gagctagccg
      421 ctaacgcgcg gctcgcatct gcgcgtacag cttgtcgtag agctcgagcc gcgccttcgc
      481 cggcaaatgc tccgagctcg gatcggcgtc gagaatgatc gcgcgatcgc tctcgccgac
      541 cctgtgctct tgccaggtcg gtagcttagg accgttcgga ttgccggtgc gcgcgaagtt
      601 gacccagtac gacgacattt ggtccgcgac ctttcggtcc ggcgccgacg ccgccgcgag
      661 cgcaggatcg ctcgggtcgg gaaaaagcgg cgccttgccc aagttgttga acacgtacgg
      721 cagctcggac gcgtgtgccg cggggaccgc gggttgcccc ggcggtcccg gcgggttctg
      781 cgcgaaatag aacagccacg cctgccggcc cagcttcttc tgattgtccg cgaacagccg
      841 catgtgccag aacgcgccgt cgttggcgct atcggcttgc gacttggtcg cctcctcatt
      901 cgtcgcgtgc ggatagagcg taaggaactc gtccgccagc gcgccccagc gcggagccgc
      961 gccctgctcg aattgctcgg gcgtgacggg tcgcgggggg ccgaacgaca agtcgtctcg
     1021 gttcgagccg acgaggacgt cgacgggatt ctgcttgccc gcggcgaaca cgtcggacgg
     1081 atcctcggga atgatccagc cgtcgacgat catgcccccg ccgccgatgc cgcggccgct
     1141 gagcttcgcc gtgacgtcgg cggccggcat cgcgcgcagc tgctcgaccg tcgtgacgcc
     1201 ggccgcgtcg gcgaggcctt tgccggcgct ttccgcgcgc gcgcgcgtcg cgaacgcggc
     1261 cgaggggccg agtcccatcc acgagccgct ctggccgatg gctcgctggt acaggccttt
     1321 cgtcaccggt gacgacgtca gcgcgccgat cgccatcgcg ccggccgact ggccgaacac
     1381 ggtcacgttg ccgggatcgc cgccgaacgc ggcgatgttg ctcttgaccc agttgagcga
     1441 ggcgaccatg tcggcgaggc cgtaattgcc cgaagacttg tgcggcgagt cggccgtcaa
     1501 cgcgggatgc gcgaagaagc cgaacggccc gagccggtag ttcatcgtga cgacgacggc
     1561 ccccttcttc gcgagcgcgt cgccgtcgta gagcgggatc gagccggcgc cttccgaaaa
     1621 ggcgccgccg aagaaataga ccatgacggg ccggcgctcg gttgcggatt gcgcgccggt
     1681 ccagacgttc agataaagac agtcttcgga ctgcggcggc gagccgggcc cgaccgcgat
     1741 gtttccgcgc gcgccgccgt ccggctgaag gcagacgttg ccgaacttgc tcgcgtcgcg
     1801 cacgccgttc catttcgcga cgggctgcgg ctcgcgccaa cgcagcgcgc cgacgggcgg
     1861 ggccgcgaac gggatgcctt tgaaaacgcg gacgcccggc gacgactcgg tcgagttgcc
     1921 gagcgagccc gcgtcgagct tgatcggctc gcgaagctgc gcgtttgcga cgccgcaaag
     1981 gataagcccc gcggccgcca acaggccggc tcgcccggcc gcgttcactt tcttcattgt
     2041 tttttcgctg cctgtgcgcg ctcgtagcgc gaagtgacga tgtaattgcg caccgccgtg
     2101 ttgtcctcgt ggcacgcgta ctcgaa
//
LOCUS       DASSNN010000794         3677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1004759, whole genome shotgun sequence.
ACCESSION   DASSNN010000794 DASSNN010000000
VERSION     DASSNN010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004759"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /gene="glnD"
                     /locus_tag="VFL84_14920"
     CDS             complement(<1..1593)
                     /gene="glnD"
                     /locus_tag="VFL84_14920"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HET7609972.1"
                     /translation="MRNPEDTQPPLIDRAALEQALRGDVRAVKAFRDALKNANDQLAE
                     RFRRNEPVETLVADRAHVVDEVILASWRCFAENVLDSADLVAVGGYGRGELHPQSDID
                     LLVLIESASNKTDDAIGRFLTFLWDIGLEVGHSVRTLDDCEAQARGDVTVLTTLMETR
                     LLHGPGQLFAELPVRIAKDRMWDSVEFFHAKTEEQRTRHARYHDTAYNLEPNVKGSPG
                     GLRDIQMIGWLAQRHLGTRTLEELVPHKFLTPGQLRILTQGRAFLWRIRFGLHLLTGR
                     REDRLLFDHQVKLAQMLGYEDASFTLAVEQLMQRYYRTVMELSRLNEMLLQLFEQAIL
                     LDENAPPIPLGPRFQSRNGYLEVTDDQVFAREPSALLEIFLLLEQNLDLRGVNARTIA
                     LVKQHLWLIDEEFRQNPRHHRLFLDILRAPQGVTRTLKRMNTYGVLGLYIPAFGRVVG
                     RMQYDLFHAYTVDAHTLFVVENLRRFALPRFDKEFPSCSAAMQRLEKPEIAYLAGLFH
                     DIAKGRGGDHSELGAVDAESFCL"
     gene            complement(1597..2382)
                     /gene="map"
                     /locus_tag="VFL84_14925"
     CDS             complement(1597..2382)
                     /gene="map"
                     /locus_tag="VFL84_14925"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET7609973.1"
                     /translation="MITIKTPEEQEKMRVAGRLAAQVLDMIEPHVQAGVSTEELDRLC
                     HEYIVGEIGGIPAPLNYRGSGGAAPFPKSICTSVNHVVCHGIPSDRTLRNGDIVNIDV
                     TVIKDGYHGDTSRMFMVGAPSIQARRLTNVCFDAMWRGIRAIRPGGRLGDVGHAIQSF
                     VEEQRFSVVREYCGHGIGRGFHEDPQVLHYGRPGTGPELVPGMTITVEPMVNAGKRDV
                     KLLSDGWTVVTKDHSLSAQWEHTVLVTDVGYEVLTLSPAAQAA"
     gene            2718..3596
                     /gene="rpsB"
                     /locus_tag="VFL84_14930"
     CDS             2718..3596
                     /gene="rpsB"
                     /locus_tag="VFL84_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET7609974.1"
                     /translation="MADVTMRRMLEAGVHFGHQTRFWNPKMAEFIFGERNKIHIINLE
                     QTMPRYKQAAEFVRKLVADGGTLLFVGSKRAAREAIATEAARCGMPYVTQRWLGGMLT
                     NFKTIRQSIKRLKSLEEITEPGAVTTFTKKELLKLSREKEKLEKTLGGIKNMDALPTA
                     VFLIDVGHEEIAVREARKLGIPVVAVVDTNCSPDGIDYPIPGNDDAMRAIQLYAEGIA
                     DAVIEGKSSIPDVPTGDDEFVELDESGKPKVEAKPQRKAPPKKRARVTKVRSKGRARP
                     TDDAPAEGGDEEPEGE"
BASE COUNT          652 a         1156 c         1266 g          603 t
ORIGIN      
        1 gaggcagaag gattcggcgt cgacggcgcc gagctcggag tggtcgccgc cgcgcccttt
       61 agcgatgtcg tggaacaggc cggcgaggta cgcgatctcg ggcttctcga gccgctgcat
      121 ggccgcgctg cacgacggaa actctttgtc gaaccgcggc agcgcgaagc gccgcaagtt
      181 ctcgacgacg aacagcgtgt gggcgtcgac ggtatacgcg tgaaacaggt cgtactgcat
      241 gcggccgacg acgcggccga aggccgggat gtacaggccg agcacgccat aggtgttcat
      301 gcgcttcagc gtgcgcgtga cgccctgcgg cgcgcgcagg atgtcgagga acaaccggtg
      361 gtggcgaggg ttctgccgga actcctcgtc gatgagccac agatgctgct tgacgagcgc
      421 gatcgtgcgc gcgttgacgc cgcgcaggtc gaggttctgc tcgagcagca agaagatctc
      481 gagcagcgcc gacggctcgc gcgcgaagac ctggtcgtcc gtgacctcga ggtagccgtt
      541 gcggctctga aagcgcgggc cgagcggaat gggcggcgcg ttctcgtcga gcaggatcgc
      601 ttgctcgaag agctgcagca gcatctcgtt caagcggctc agctccatga cggtgcggta
      661 gtagcgctgc atgagctgct cgacggcgag cgtgaagctc gcatcctcgt agccgagcat
      721 ctgcgcgagc ttgacctgat ggtcgaacaa gaggcgatcc tcgcgccggc cggtcaggag
      781 atgcaggccg aagcggatgc gccagagaaa cgcgcgccct tgcgtcagga tgcggagctg
      841 cccgggcgtc aggaacttgt gcggcacgag ctcctcgagc gtgcgcgtgc ccaagtgccg
      901 ctgcgcgagc cagccgatca tctggatgtc acgtaagccg ccggggctgc ctttgacgtt
      961 cggctcgagg ttgtacgccg tgtcgtggta gcgcgcgtgg cgggtccgct gctcctcggt
     1021 cttagcgtgg aagaactcga cgctgtccca catgcggtcc ttcgcgatgc gaaccggcag
     1081 ctccgcgaac agctgccccg gcccgtgcag cagccgcgtt tccatgagcg tcgtcagcac
     1141 ggtcacgtcg ccgcgcgcct gcgcttcgca gtcgtcgagc gtgcgcacgc tgtggccgac
     1201 ctcgagcccg atgtcccaca gaaaggtcag gaatcggccg atcgcgtcgt cggtcttgtt
     1261 cgatgcgctc tcgatcagca cgagcaggtc gatgtccgat tgcgggtgca gctcgccgcg
     1321 gccgtaaccg ccgacggcga cgaggtcggc cgagtcgagc acgttctcgg cgaagcagcg
     1381 ccagctcgcg aggatgactt cgtcgacgac gtgcgcgcgg tcggccacga gcgtctcgac
     1441 gggctcgttg cgccgaaagc gctcggcgag ctgatcgttc gcgttcttca gcgcgtcgcg
     1501 gaacgccttc acggccctga cgtcgccgcg cagcgcttgc tcgagcgcgg cgcgatcgat
     1561 cagcggaggt tgcgtgtcct cgggatttct catgcgtcag gcggcttgcg cggcggggct
     1621 caacgtcagc acctcgtagc ccacgtccgt gacgagcacc gtgtgctccc actgcgcgga
     1681 cagcgagtgg tctttcgtga cgacggtcca gccgtccgac aagagcttca cgtcgcgctt
     1741 gccggcattg accatcggct cgaccgtgat cgtcatgccg ggcacgagct cggggcccgt
     1801 gccgggccgg ccgtagtgca gcacctgcgg gtcctcgtga aacccgcggc cgatgccgtg
     1861 cccgcagtat tcgcgcacga ccgagaagcg ctgctcctcg acgaagctct ggatcgcgtg
     1921 accgacgtcg cccaagcggc cgccgggccg gatcgcgcgg atgccgcgcc acatggcgtc
     1981 gaagcagacg ttcgtgagcc gccgcgcctg tatcgacggc gcgccgacca tgaacatgcg
     2041 gctcgtatcg ccgtgatagc cgtctttgat gacggtgacg tcgatgttga cgatgtcgcc
     2101 gttacgcagc gtgcgatcgc tcggaatgcc gtggcagacg acgtggttga ccgaagtgca
     2161 gatcgatttc gggaacggcg cggcgccgcc cgagccgcga tagttcagcg gcgcggggat
     2221 cccgccaatc tcgccgacga tgtactcgtg gcagaggcga tcgagctcct cggtggagac
     2281 gccggcctgg acgtgcggct cgatcatgtc gagcacctgc gcggccaaac gcccggccac
     2341 gcgcatcttc tcctgctctt caggcgtttt gatcgtaatc atggtcgggt ttggggtcgg
     2401 tgccggtggt acgggaaaag gggacggatc tattttccgc ctacgtacgt tggcttggcg
     2461 acagatctac gcgcggaaaa tagatccgtc cccttttccc gtaagccggt acctcctgtg
     2521 ggtcggcaaa cctgcgccgc gcaagcgaat tcacgttgtt tgccgcaagg ggcgtatggt
     2581 ataaagcgcg cgcctcggag gcaaaccacc gccggggtaa atacacacac gcgcctaaca
     2641 ctgcgggcgg ggtgcctcag gcttctgggg tcgttcgcag aaacgcgtgg aggaccaacc
     2701 caaacaagga gctttccatg gccgacgtca ccatgcgccg gatgctggaa gcgggtgtgc
     2761 acttcgggca ccaaacccgt ttctggaatc ccaaaatggc cgagttcatc ttcggcgagc
     2821 gcaacaagat ccacatcatc aatctcgagc agacgatgcc gcgctacaag caggcggccg
     2881 agttcgttcg caagctcgtc gcggacggcg gcacgctgct gttcgtcggc agcaagcgcg
     2941 ccgcgcgcga ggcgatcgcg accgaggccg cgcgctgcgg aatgccttat gtcacgcagc
     3001 gctggctcgg cggcatgctc acgaacttca agacgatccg ccagtcgatc aagcgcttga
     3061 agtcgctcga ggagatcacg gagcccggcg cggtcaccac cttcacgaag aaggagctgc
     3121 tgaagctgag ccgcgagaag gagaagctcg agaagacgct cggcggcatc aagaacatgg
     3181 acgcgctgcc gaccgcggtg ttcctgatcg acgtcggaca cgaggagatc gccgtgcgcg
     3241 aagcgcgcaa gctcggcatc cccgttgtgg ccgtggtcga cacgaactgc tcgcctgacg
     3301 gtatcgacta cccgattccc ggcaacgacg acgcgatgcg cgccattcag ctgtacgccg
     3361 aagggattgc ggacgccgtg atcgagggca agtcgtcgat cccggacgtg ccgaccggcg
     3421 acgacgagtt cgtcgagctc gacgagagcg gcaagccgaa ggtcgaggcg aagccgcagc
     3481 gcaaggcgcc gccgaagaag cgcgcgcgcg tcacgaaggt ccggtcgaag ggacgagcgc
     3541 gcccgaccga cgacgcgccg gccgaaggcg gcgacgaaga gccggaaggc gaatagtttt
     3601 taacgaattt gaggagaggg ttccggtatg gacatcaaac ccgccttggt caaagagctg
     3661 cgcgagcgca cgggcgc
//
LOCUS       DASSNN010000795         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_377122, whole genome shotgun sequence.
ACCESSION   DASSNN010000795 DASSNN010000000
VERSION     DASSNN010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377122"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VFL84_14935"
     CDS             <1..746
                     /locus_tag="VFL84_14935"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET7609975.1"
                     /translation="IRSTGCERRAPAATRVESDAALDMAAGLIARGSKIDAALIKSGF
                     RATSATSINVGGVSGDAAIREVLAGGFCDKVNNEAYTAIGAYVRGKELWVVLAERFEA
                     VAASDSSAVAARVLALVNDARKHERRCGRKKMSATHALTPSAALTRAAAAHAADMAKN
                     GFMGHRGSDGSEAGERVRRAGYVWRSVGENVAAGQPDAEAVVKAWLESPGHCTNIMGP
                     QFTQMGVAFALAPRSELRLLWAQELGAPQ"
     gene            complement(1123..>2380)
                     /locus_tag="VFL84_14940"
     CDS             complement(1123..>2380)
                     /locus_tag="VFL84_14940"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7609976.1"
                     /translation="CKGLEPIDDQLFEANGQQIQASGHGRCLAVEALVPGQPLHMREC
                     SDSRLQRWRFAAGRLSPAARPELCVALASARGEPWGTPRLVSPAYRRQAIALDKCAAE
                     ASTRQAFRWLAPNEVASSRADDARAGMPPELATALAAFGHEFDGAIAQQTAALYAPQP
                     RVYAQEEITVTKDLAYGPHERQHVDVYTRARRPAEPVPAIVVFHGGGLIGGNRGATAN
                     VAEYFASLGYVGVNGGYRLAPEAKWPEGARDVAGAVTYLRDHVAEYGGDPSRIFVAGI
                     STGALHAATYVFRPELLQAGAARAAGAILVSGPYTFDFDAAGRGELAYFGEDRARWPE
                     MVVTGNITRTDIPVLMTTAEWDNARYTKAYAVLFHELVVEHGVVPRYFQSLGHNHSSQ
                     LLSVGTTDRSVSATIVDFIERTAHSR"
BASE COUNT          357 a          786 c          855 g          382 t
ORIGIN      
        1 acatccgctc gacggggtgc gagcgccggg cgcctgccgc gacgcgcgtc gagagtgacg
       61 ccgcgctcga tatggcggcc gggctcatcg cgcgcggcag caagatcgac gccgcgctga
      121 tcaagtccgg ctttcgagcg acgagcgcga cgtcgatcaa cgtcggcggc gtctcgggcg
      181 atgcggcgat tcgcgaagtg ctggccggcg gtttctgcga caaggtcaac aacgaggcat
      241 acacggcgat cggagcgtac gttcggggca aggagctgtg ggtcgtgctc gcagagcgct
      301 tcgaggcggt agccgcaagc gattcaagcg ccgtcgccgc tcgagtgctc gcgctcgtca
      361 acgatgctcg aaagcacgag cgccgttgcg gccggaagaa gatgagcgcg actcacgcat
      421 tgacgccgtc ggcggcgctg acgcgagccg cggccgcgca tgccgccgac atggccaaga
      481 acggttttat gggtcatcgc ggctccgacg gcagcgaggc cggcgagcgt gtgcgccgtg
      541 ccggatacgt gtggcgctcg gtcggcgaaa acgtggcggc cggtcagccg gatgccgagg
      601 cggtcgtcaa ggcatggctc gaaagcccgg ggcattgcac gaacatcatg ggtccgcagt
      661 tcacgcagat gggcgtcgcg ttcgcgctcg cgccgcggag cgagctgcgc ctactgtggg
      721 cgcaggagct cggcgcaccg cagtagcgct cacgcggaac tacgtgtcac gtgaagtggt
      781 gggcccggta ggactcgaac ctacgaccaa gggattcact tcgcccgccg tttccggcgg
      841 aagtggacta tctcttcacc cgccgagttt caagtcggtt tgggtgcggg acgctcgcgc
      901 ctgtcattaa gagcactgaa gctctcaggt agtctctgca ccgtccggcg gtgtaccgcc
      961 ggcttggctc agggttgccg gcgcgcgtcg tgcgcgttgc ggttcccctg aattcatccc
     1021 gtccacttcg cggctcgtcg ccgcgaaggc accttttacg atgagtcccc tgctctaacc
     1081 aactgagcta cgggcccacg cgcgtatcgt acaccggcgg cttcagcggc tgtgcgcggt
     1141 gcgctcgatg aagtcgacga tcgtggcgga tacgctgcgg tccgtggttc cgacggacaa
     1201 gagctgcgac gagtggttgt ggcccaggct ctggaagtag cgcggcacga cgccgtgctc
     1261 gacgacgagc tcgtggaaga gcacggcata ggctttcgtg tagcgcgcat tgtcccattc
     1321 ggcggtcgtc atcagcaccg ggatgtcggt gcgagtgatg ttgcccgtga cgaccatctc
     1381 gggccagcgc gcgcggtctt cgccgaaata cgcgagctcg ccgcggccgg ccgcgtcgaa
     1441 gtcgaacgtg taggggcccg acacgaggat cgcgccggct gcgcgtgcgg cgccggcctg
     1501 cagcagctcg gggcgaaata catacgtcgc cgcgtgcagc gcgcccgtcg agatgccggc
     1561 gacgaatatc cggctcgggt cgccgccgta ttcggcgacg tggtcgcgca gatacgtgac
     1621 cgcgcccgcc acgtcgcggg caccctcggg ccatttcgcc tcgggggcca agcggtagcc
     1681 gccgttgacg ccgacgtagc cgaggctcgc gaagtactcg gccacgttgg cggtcgcgcc
     1741 gcgattgccg ccgatgagcc cgccgccatg aaacacgacg atcgcgggca ccggctccgc
     1801 gggccgacgc gcgcgcgtgt agacgtcgac gtgctggcgc tcgtgcggcc cgtaggccaa
     1861 gtccttcgtg accgtgattt cttcttgcgc gtagacgcgc ggctgcggcg cgtatagcgc
     1921 ggctgtctgc tgcgcgatcg cgccgtcgaa ctcgtggccg aacgcggcca atgccgtcgc
     1981 gagctcgggc ggcatgcccg cgcgcgcgtc gtcggcgcgg ctcgaggcca cttcgttcgg
     2041 agcaagccag cggaacgctt gccgcgtgct cgcttcggcg gcgcatttat cgagcgcgat
     2101 cgcctgccgg cgatacgccg gcgagacgag ccgcggcgtg ccccagggct cgccgcgcgc
     2161 ggacgcgagc gcgacgcaga gctcgggacg cgcggccggg ctcaagcgcc cggccgcgaa
     2221 tcgccagcgt tggagccgcg agtccgagca ctcgcgcatg tgtaaaggct gccccggcac
     2281 caacgcttcc accgcgaggc agcggccgtg cccgctggcc tggatctgct gtccgtttgc
     2341 ttcgaagagc tgatcgtcga tcggctcgag gcccttgcac
//
LOCUS       DASSNN010000796         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_377305, whole genome shotgun sequence.
ACCESSION   DASSNN010000796 DASSNN010000000
VERSION     DASSNN010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377305"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..694
                     /locus_tag="VFL84_14945"
     CDS             257..694
                     /locus_tag="VFL84_14945"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HET7609977.1"
                     /translation="MRLRPILLWLAAPLAGCAGTATTDDGRDRTAEVRDTDCLRTPLI
                     TNWEALDEHNLIVYESRRPYHVELARTCSGLDFATLIGFYDRGGDERICGYGLDRVIV
                     DRFIPEGCTVTAVDELTDEQAADLKRRAEEEKALARPRPRARR"
     gene            complement(609..1535)
                     /locus_tag="VFL84_14950"
     CDS             complement(609..1535)
                     /locus_tag="VFL84_14950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET7609978.1"
                     /translation="MSTTTERRRIARESSDAPPSGWWNVARRVKDDVAADNVSMIAAG
                     AAFFGLLALFPALAAAVALYGLVTDPSTVSAHLDLLSGFVPAEARMILDEQLQRITAS
                     ANTSLGIGAVVALLVATWSAAKGVKSLMTALDVVYHEREDRGFFKLNGTALLLTIAML
                     VVVPLSLAVVAVLPALIDRLPLPDLVATAARWLRWPLIAAVSVAAMTMLYRLGPAHTE
                     GPKPSLPGAIAATILWLIASALFSWYVSTFGSYNETYGSVAAMAVIMMWFWLSAFAVL
                     IGGEINAELAAAAARAPSPPRRDVSDRRPARR"
     gene            1679..2098
                     /locus_tag="VFL84_14955"
     CDS             1679..2098
                     /locus_tag="VFL84_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7609979.1"
                     /translation="MNIQDVMTPDVSFVGPETPILEIARKMRDGDIGATPVVENDRLA
                     GMVTDRDIVVRVIAEGGDVRTKTARDAMSPGILYCYVDDTVETVLENMGDQQIRRLPV
                     VDRDKRLVGVVSLGDLALSGKRKAAGEALQEISQPTH"
BASE COUNT          378 a          697 c          713 g          327 t
ORIGIN      
        1 cgtgcaattg gcgtctcgct cgtcggagtc gtcatcgtcg tcgtcgtcct gggctttgac
       61 ggcgccggca tgcagcgctg cggcgaccaa caaagaggca aacactagcc gataggtacg
      121 catggccgtc tcgtccttga gatctcgccc gaagcgcgtg caagactcgg gcccggcggc
      181 gccgtacgcc cgcagtgctt ggcacagccc ttgcactttt gtgggttcat ccccgagccg
      241 taacaaggag gttgcgatga gactgcgccc gatattgctc tggctggcgg cgccgctcgc
      301 cggctgtgcc gggaccgcga cgaccgatga cggacgagac cgcacggcgg aagtgcgcga
      361 taccgattgc ttgcggacac cgctgatcac caattgggaa gcgctcgacg agcacaatct
      421 gatcgtgtac gagtcgcggc ggccctatca cgtcgagctc gcgcgaacgt gctccggcct
      481 cgactttgcg acgctgatcg gcttctacga tcgcggcggc gacgagcgca tttgcggcta
      541 cggcctggac cgcgtgatcg tcgatcgttt cattcccgag ggctgcacgg tcacggccgt
      601 ggacgagctc accgacgagc aggccgccga tctgaaacgt cgcgccgagg aggagaaggc
      661 gctcgcgcgg ccgcggccgc gagctcggcg ttgatctcgc cgccgatcag cacggcgaat
      721 gccgagagcc agaaccacat catgatcacg gccatcgccg cgacggagcc gtacgtctcg
      781 ttgtagctgc cgaacgtcga gacgtaccac gagaacagcg ccgaggcgat cagccacaag
      841 atcgtcgctg cgatcgcgcc cggcagcgag ggcttcggcc cttcagtgtg tgccggcccg
      901 agcctgtaca gcatcgtcat ggccgccacg gagaccgctg cgatgagcgg ccaacgcagc
      961 cagcgcgccg cggtcgcgac gaggtctggc aacggcagcc gatctatcag agcgggcagc
     1021 accgcgacga cggcgagcga cagcggcacg acgacgagca tcgcgatcgt cagcagcagc
     1081 gccgtgccgt tcagcttgaa gaagccgcgg tcttcgcgct cgtgatagac gacgtcgagc
     1141 gcggtcatca gcgacttcac gcccttggcc gcgctccaag tcgcgaccaa cagcgcgacg
     1201 accgcgccta tgccgagcga agtgtttgcc gaagccgtga tccgctgaag ttgctcgtcg
     1261 agaatcatgc gtgcctcggc cggaacgaag ccggagagga gatccaagtg cgcgctgacc
     1321 gtcgacggat cggtgacgag gccgtacaga gcgacggccg cggcgagcgc gggaaagagc
     1381 gcgaggaggc cgaagaacgc ggcaccggcc gcgatcatgg agacgttgtc ggcggccacg
     1441 tcgtccttga cgcgccgcgc gacattccac cagccgctcg gcggcgcatc gctcgactcg
     1501 cgcgcgatgc ggcgacgttc cgttgtcgtg ctcattcggg tccttcctcg cgcgggggca
     1561 tttttgcaag gagtgtgcca gcgccagccg ggcaagcgcg cgggtggcat gccgcttgca
     1621 ctggcttcgg tcgctctgag tccaccacgt tccggccgtt ggccgaggga agcgtgccat
     1681 gaacattcaa gacgtcatga cgcccgacgt gagcttcgtc gggcctgaga ctccaatcct
     1741 ggaaatcgct cgcaaaatgc gcgacggcga tatcggtgcg acgcccgtcg tcgagaacga
     1801 ccggctcgcc ggcatggtga cggaccgcga catcgtcgtg cgcgtcatcg ccgagggcgg
     1861 cgacgttcgg accaagaccg cgcgcgacgc gatgtcgccg ggcattctct actgctacgt
     1921 cgacgacacg gtcgaaaccg tgctcgaaaa catgggcgat cagcagatcc gccgcctgcc
     1981 ggtcgtcgac cgcgacaagc gcctcgtcgg cgtcgtgtcg ctcggcgatc tcgcgctgtc
     2041 cggcaagagg aaagcggcgg gcgaggcgct gcaagagatt tctcaaccca cgcattgagc
     2101 ggcgacccgc ccaac
//
LOCUS       DASSNN010000797         3481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1005564, whole genome shotgun sequence.
ACCESSION   DASSNN010000797 DASSNN010000000
VERSION     DASSNN010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005564"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="VFL84_14960"
     CDS             <1..1362
                     /locus_tag="VFL84_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7609980.1"
                     /translation="GIVAPGDAAPLLRDERGLYRGAAALVVRPSSVDECAKVVRVCND
                     ARIGVVPQGGNTGYCGGATPFDGERRQILLSLARLNRVREVDDVGFTMTADAGVVLAR
                     AQDAARERGLKFPLSMGSEGSAQIGGALATNAGGVAVLRYGTMRDLVLGLEVVLPSGD
                     VLAELKGLRKDNTGYDSKALFLGAEGTLGVITAAVLKLFPEPRAVATAWLAVASVDAA
                     CRLLGRARRESGDQVVSAEYVSRRSLELVLRHVEGAREPLGSPAEHYVLLELASADDD
                     EALREKLTRVLATGLEAGEIVDGTLAESEAQRRALWLLRERVPEAERREGGSVKHDIS
                     VRIGRIPEFLERAEPALRGIGPHRLSIYGHIGDGNLHCNVLPPVGQSSEAFRAEKGAA
                     FSACVHDVCAALDGSFSAEHGVGILKVDELARYESPTALALMRALKAALDPHGIMNPG
                     KVL"
     gene            1359..1904
                     /locus_tag="VFL84_14965"
     CDS             1359..1904
                     /locus_tag="VFL84_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HET7609981.1"
                     /translation="MTFRTLVAHTQGGTPAAKIVARAGLDRGRRMTLEWSLTGDLKAL
                     AIPRRSASRRADRLWEHTCFEAFVAPASGTRYCELNFAPSTRWAAYELDGYRQGMRPL
                     ELATPPTVKVVTAANELRVTAGVELGGFADAPWPWRIGLTAVVEDRAGGRAFYALLHP
                     RAKPDFHDAAAFTVILDGSAR"
     gene            complement(1873..2964)
                     /locus_tag="VFL84_14970"
     CDS             complement(1873..2964)
                     /locus_tag="VFL84_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HET7609982.1"
                     /translation="MIQVYRYRVKNLAGLLHRQALAVNCVWNYCNDVQVSALTWNKRW
                     PSGFDLNVLTTGSSKELGLHSGAVNAVCEQYAKSRNQQKRPFLRYRGRRSLGWVPLKG
                     RDLKREGNAFRFAGNTFRVWYSRPLPHGKIKDGTNFAQDSRGRWYLNIAVEIPNVEPR
                     AIEREVGIDLGLKDLAVLSTGEKIENPKQLAKLADKLSQAQRANKKRLATNIHARIKN
                     ARNDFLHKASHRIVRDFDYIAVGNVNAASLAKTRLAKSVLDAGWSSFRNMLRYKAIAR
                     GAWYEEVNERFSTQVCSACGSMPEQRPKGIADLGIREWMCGDCGSVHDRDQNAALNIL
                     RVGHHSPVVGIPGLWPGEHVNEHSRRESQ"
     gene            3095..>3481
                     /locus_tag="VFL84_14975"
     CDS             3095..>3481
                     /locus_tag="VFL84_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HET7609983.1"
                     /translation="MKFGLDRLVASSALRKGLRDKRVAVLGHPASVTRDLTHALDALA
                     ALPDVKLTAAFGPQHGLRGDKQDNMVESQDFTDSEHRIPVFSLYGTVRRPTASMMSTF
                     DVLLVDLQDVGCRIYTFITTLRYVLEE"
BASE COUNT          547 a         1164 c         1157 g          613 t
ORIGIN      
        1 ggcatcgtgg cgccgggcga cgcggcaccg ctgctgcgcg acgagcgcgg tctctatcgc
       61 ggcgcggcgg cgctcgtcgt gcggcccagt tcggtcgacg aatgcgcgaa ggtggtgcgt
      121 gtgtgcaacg acgcgcgcat cggcgtcgtg ccccaaggcg gcaacaccgg ttactgcggc
      181 ggcgccactc cgttcgacgg cgagcgccgg cagatcctct tgtcgctcgc gcgtttgaac
      241 cgcgtgcgcg aggtcgacga cgtcggcttc acgatgacgg cggatgcggg cgtcgtgctc
      301 gcgcgggccc aagacgccgc gcgcgagcga ggcctcaagt tcccactcag catgggatcc
      361 gaaggctcgg cgcagatcgg cggcgcgctc gcgacgaacg caggcggcgt cgcggtgctg
      421 cgctacggca cgatgcgcga cctcgtgctc ggcctcgagg tcgtgctgcc gagcggcgac
      481 gtgctcgcgg agctcaaggg gctgcgcaag gacaacacgg gctacgattc gaaggcattg
      541 ttcctcggag ccgaaggcac gctcggcgtc atcacggcgg ccgtgttgaa gctcttcccg
      601 gagccgcgcg ccgtcgcgac ggcgtggctc gccgtcgcga gcgtcgacgc ggcgtgccgg
      661 ctgctcggcc gcgcgcggcg cgagagcggc gaccaggtcg tctcggcgga atacgtctcg
      721 cgccgctcgc tcgagctcgt gctgcgccac gtcgaaggcg cgcgcgagcc gctcggctcg
      781 ccggccgagc actacgtgct gctcgagctc gcgtcggccg acgacgacga ggcgctgcgc
      841 gagaagctca cgcgcgtgct cgcgaccggt ctcgaagcgg gcgagatcgt cgacggcacg
      901 ctggcggagt ccgaagcgca gcgccgcgcg ctgtggctgc tgcgcgagcg cgtgcccgag
      961 gcggagcgcc gcgagggcgg ctccgtcaag cacgacatct cggttcgcat cggccggatt
     1021 cccgaattcc tggagcgcgc ggagcccgcg cttcgcggca tcggtccgca tcggctgtcg
     1081 atctacggcc acatcggcga cggcaacttg cactgcaacg tgctgccgcc ggtggggcag
     1141 agcagcgaag cgtttcgtgc cgagaaaggc gccgcgttct cggcttgcgt gcacgacgtc
     1201 tgtgccgctc tcgacggcag cttcagcgcc gagcacggcg tcgggattct gaaagtcgac
     1261 gagctcgcgc gctacgaatc gcccaccgcg ctcgccttga tgcgagcgct gaaagcagcg
     1321 ctcgatccgc acggcatcat gaatcccgga aaggtgctgt gactttccgg acgttggtcg
     1381 cgcacacgca gggcggcacg ccggcggcga agatcgtcgc gcgtgccggg ctcgaccgcg
     1441 gccggcgcat gacgctcgaa tggtcgctga ccggcgatct gaaagcgctc gcgattccgc
     1501 gccgctcggc ctcgcgccgc gcggaccggc tctgggagca cacctgcttc gaggcgttcg
     1561 tcgcgcccgc atcgggcact cgctattgcg agctcaactt cgcgccgtcg acgcgctggg
     1621 ccgcctacga gctcgacggc taccggcaag gcatgcggcc tcttgagctc gcgacacctc
     1681 ctacagtgaa ggtcgtcacg gccgcgaacg agctgcgtgt gacggccggc gtagagctcg
     1741 gcggcttcgc cgacgcacca tggccttggc gcatcggcct gacggccgtc gtcgaggacc
     1801 gcgcgggcgg ccgcgcgttc tatgcgctgc tgcatccgcg cgccaagccc gacttccacg
     1861 atgccgccgc gttcactgtg attctcgacg ggagtgctcg ttgacatgct ccccgggcca
     1921 gaggccgggg attcccacaa ctggcgagtg atgccccact cggagaatgt tcaacgcggc
     1981 gttttggtca cgatcatgta cggaaccaca gtcgccacac atccactctc ttattccgag
     2041 gtccgcgata cctttcggcc tctgctcggg catggaaccg caagcagaac agacctgggt
     2101 actgaatcgc tcgttcactt cctcgtacca agctccgcgc gcaatcgctt tatagcgcag
     2161 catgtttcgg aaggacgacc agcccgcatc gaggacggac ttcgccagtc tggttttggc
     2221 gaggcttgcg gcgttcacgt ttccgaccgc aatgtagtcg aagtctcgca cgatgcgatg
     2281 cgaggctttg tgcaagaagt cgttccgcgc atttttgatg cgcgcgtgga tgttggtagc
     2341 gaggcgcttc ttattcgccc gctgcgcctg cgacagcttg tcggcaagct tagcgagttg
     2401 cttggggttc tcgatctttt ctccggtaga cagtaccgca agatccttca gtccaagatc
     2461 gatgccgact tcgcgctcta tcgcccgcgg ctcgacattg ggtatctcga ccgcgatgtt
     2521 cagataccag cgcccgcgcg aatcctgagc gaagttggtg ccgtctttga tcttgccgtg
     2581 cggcagcggt ctcgagtacc agacccggaa ggtattgcct gcgaatcgaa acgcgttccc
     2641 ttcgcgcttg aggtctctgc ctttcaacgg aacccagccg aggctgcggc gcccgcgata
     2701 gcgcaagaac ggccttttct gctggttgcg ggacttggcg tattgctcac agaccgcgtt
     2761 cacggcgccc gagtgaaggc cgagctcctt gctcgatccg gtcgtgagga cgttcagatc
     2821 gaagccgctc ggccatcgct tgttccaggt cagcgccgac acctgcacgt cgttgcagta
     2881 gttccagacg caattgactg caagcgcttg ccggtgaagc aggcccgcga gattcttgac
     2941 gcggtagcgg tagacctgaa tcattctcgg cactatcgca cggcgctcaa gcgatacgtc
     3001 aaggcgcagg gcgcgtccac ggctttccaa ccacccacaa cacggagcgg cgcttcctcc
     3061 ccgtcgtaac cgacggttcc gcgccgaaga ttcgatgaaa ttcggcttgg atcggctcgt
     3121 cgcttcgagc gcgctgcgca agggtttgcg cgacaagcgc gtcgcggtgc tcgggcaccc
     3181 cgcgtcagtg acgcgcgacc tcacgcacgc gctcgacgcg ctcgcggcgc tgcccgacgt
     3241 caagctcacg gcggccttcg gcccgcagca cggcctgcgc ggcgacaagc aagacaacat
     3301 ggtcgagtcg caagatttca cggactccga gcaccgcatc ccggtcttca gtctctacgg
     3361 cacggtgcgg aggccgacgg cttcgatgat gagcacgttc gacgtgctgc tcgtcgatct
     3421 gcaagacgtc ggttgccgca tctacacctt cattacgacg ctacgctacg tgctcgagga
     3481 a
//
LOCUS       DASSNN010000798         6202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_377646, whole genome shotgun sequence.
ACCESSION   DASSNN010000798 DASSNN010000000
VERSION     DASSNN010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377646"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFL84_14980"
     CDS             complement(<1..664)
                     /locus_tag="VFL84_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609984.1"
                     /translation="MKTALVLLVASLGLANASAAAGKRADLDGVWLFYGSQVPKDPGL
                     TPAGKEKLAQYDPLRDDDDTSCKPVTFTNIMHTPSPPIEVRQRDDHIEINYEFMDVHR
                     RVPLWGTLADAPYTVADHPHMGRSIGRYEGDELVIDTADVAAGYLDTLGFPGLPQSAE
                     MRTEERFIPAGDRLTVVVTHHDPVNYVQPMVMTYEFVRVDTEVMPWGCTVEGANYDER
                     LKS"
     gene            complement(661..1128)
                     /locus_tag="VFL84_14985"
     CDS             complement(661..1128)
                     /locus_tag="VFL84_14985"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7609985.1"
                     /translation="MKSRHRVFLFAGLTLLAALSAAAHHSVAVNFERGEPIEIRGTVK
                     EAHMRNPHSQYVVDVRAEDGTLTEWLVEWSDRNSLVRRGVDLDLIKPGDTVTFALWPS
                     QRLPNVGYFVQAVLADGSIYRDCGFVQYREAVAKSAKFRCEEGTRDNVQRPTQ"
     gene            1295..1828
                     /locus_tag="VFL84_14990"
     CDS             1295..1828
                     /locus_tag="VFL84_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609986.1"
                     /translation="MTRTIHAQIGLVIGAVALTAMAGVSAQAQEAQQAEGARRTLSEA
                     EKAQAELDRTPEDCVLLNRVDRNIAASDRQVVFFMKGGKNYLNVLDQSCPALKAGDYR
                     LVFHYRTASAKTTRLCDYDAFTVERQTSRVGCSLGPFIPITPEEAAALTAKPAAAPPA
                     SNAGGSGGNSGGDNRNR"
     gene            1973..2308
                     /locus_tag="VFL84_14995"
     CDS             1973..2308
                     /locus_tag="VFL84_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7609987.1"
                     /translation="MGKDAPEHISTRIISQRELIVDGAPFQWWAVGERPALVTVRSNV
                     FGSLTKFTDGDVAEYAASLAKELLAQHYARAGRSPAGKRLPTEATTGLNKPGWFEPGD
                     AVDFSTTVF"
     gene            complement(2313..3059)
                     /gene="modA"
                     /locus_tag="VFL84_15000"
     CDS             complement(2313..3059)
                     /gene="modA"
                     /locus_tag="VFL84_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014291959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7609988.1"
                     /translation="MRKRWLACVGVLIASWVSRQAVADEITIAVATNFTAAMRDLVQR
                     FELETKHHVLVSSGSTGNYYAQIKNGAPFEAFFAADAERPRLLEQEGVAVPGSRFRYA
                     VGRLVLWSPEPNYVDDQGRVLEKAGFRRLSIANPDLAPYGAAARETLTARGLWERLMP
                     RLAMGQDIGQAYAFVQTRNAELGFVALAQLRQPGKQIEGSYWIVPESLHRPIEQEAVL
                     LREVPAARMFLEFVKGAEAREIIRSYGYEP"
     gene            3228..3605
                     /locus_tag="VFL84_15005"
     CDS             3228..3605
                     /locus_tag="VFL84_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HET7609989.1"
                     /translation="MAEPKLSKLELKIMEALWAKGPSSVREIQESFPAKRRPAYTTVQ
                     TIVYRLEAKNALKRTKKIGNAHIFEAVVSKTAAHRKLIDDVLGLFGGRAQPLMAHLIE
                     SGQLTLADIEDARKALRDTAKKE"
     gene            3611..4864
                     /locus_tag="VFL84_15010"
     CDS             3611..4864
                     /locus_tag="VFL84_15010"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HET7609990.1"
                     /translation="MLNEIVNHVWQSTLVAAAIAALAALLRGDSAHSRYWLWWAASLK
                     FLVPFSLLAALGAALRDAGAPRVELADWPATLGALAEPMPEAAGWAALPAALAAIWAT
                     GFAAVAGAWTVRALKVRALLRSASPYAGVLPRGAGRLEARTSTALLEPALVGIARPVL
                     LLPHGIAEHLTRSQLDAVLEHELTHWRRRDNLTAAVHMVVEAVFWFYPLVWWIGARLV
                     EERERACDEAVVRAGHDGRAYAEGILNVCERYVASALKCAAGISGADLKRRVVEIARN
                     RVMSELPFQKKALLGAVALATLIVPIIFGAAAQDGADPIPILRIQPNYPEDALVAGRE
                     GSVDLEFTIAANGSVKDVVVVSSTAPEFEEPAVKALLRWRFLQTNTTCTGTDCRTIEK
                     VEPVERPGMRTVIHYRLSDARPETD"
     gene            complement(4874..6058)
                     /locus_tag="VFL84_15015"
                     /pseudo
     CDS             complement(4874..6058)
                     /locus_tag="VFL84_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018148277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
BASE COUNT         1022 a         2052 c         2105 g         1023 t
ORIGIN      
        1 ccgacttcag gcgctcgtcg tagttcgcgc cctcgaccgt gcagccccac ggcatgacct
       61 cggtgtcgac gcgcacgaac tcgtaggtca tgaccatcgg ctgcacgtag ttgaccgggt
      121 cgtggtgcgt gacgacgacc gtgaggcgat cgccggccgg gatgaagcgc tcctccgtgc
      181 gcatctcggc ggactgcggc aggccgggaa acccgagcgt gtcgaggtag ccggccgcga
      241 cgtcggcggt gtcgatcacg agctcgtcgc cttcgtaccg tccgatcgag cgccccatgt
      301 gcggatggtc cgcgaccgtg tacggcgcgt ccgcgagcgt gccccagagc ggcacgcgcc
      361 ggtgcacgtc catgaactcg tagttgatct cgatgtgatc gtcgcgctgg cggacttcga
      421 tcggcggcga gggcgtgtgc atgatgttcg tgaacgtcac gggcttgcag ctcgtgtcgt
      481 cgtcgtcgcg cagcgggtcg tattgcgcga gcttctcttt gccggcgggc gtgagacccg
      541 gatctttggg gacttgcgag ccgtagaaca accagacgcc gtcgaggtcg gcgcgtttgc
      601 ccgccgccgc cgacgcgttc gcgagcccca atgacgcgac gagcaagacg agcgcggtct
      661 tcattgcgtg ggccgttgca cgttgtcgcg cgtgccctct tcgcagcgga acttcgcgga
      721 cttcgccacg gcctcgcggt attggacgaa gccgcagtcg cggtagatcg agccgtccgc
      781 gagcacggct tgcacgaagt agccgacgtt cgggagccgc tggctcggcc acaaggcgaa
      841 cgtcaccgtg tcgccgggct tgatcaaatc gagatcgacg ccgcggcgga cgagcgagtt
      901 gcggtcggac cactcgacga gccactcggt gagggtcccg tcctcggcgc gcacgtcgac
      961 gacgtactgc gaatgaggat tgcgcatgtg cgcctctttg accgtgccgc ggatctcgat
     1021 cggctcgccg cgctcgaagt tcacggccac ggagtgatgc gcggccgcgg acagcgctgc
     1081 cagcaacgtc aggcccgcaa acaagaacac gcgatgtcgt gatttcacgg ccgctccaat
     1141 gcgctttgcc ctagcgtacg ccgactgacg caagtttggg gcgcggcaat cgattcgatc
     1201 gcgcaggcgg ccccgtcgcc ctgctcgttc ttgcttgccc cggggcggcg gggatataac
     1261 gagcctgcac ggggcgagaa ctaggggggg aactatgact cgtaccatcc atgcgcaaat
     1321 cgggctcgtg atcggcgcgg tcgcgctcac ggcgatggcc ggcgtgagcg cccaagctca
     1381 agaagcgcag caagcggaag gggcacggcg gacgctgagc gaggcagaaa aagctcaagc
     1441 agagctcgat cgcacgcccg aggactgcgt cttgttgaat cgcgtcgaca ggaacattgc
     1501 cgccagcgat cggcaggtgg tctttttcat gaagggcggc aagaactacc tcaacgttct
     1561 cgaccagagc tgtccggcgc tgaaggcagg ggattatcgg ctggtctttc actaccgcac
     1621 ggcgagcgcc aagaccactc ggctctgcga ctacgacgcg ttcaccgtgg agcggcagac
     1681 gagccgcgtc ggttgctcgc tcgggccgtt cattccgatc acgcccgaag aagcggcggc
     1741 gctgacggct aaaccggcgg ccgccccccc tgcgagcaac gcgggcggca gcggcggcaa
     1801 ttccggcggc gacaaccgca accggtagcg catcggcggg cgccgaggct catgccgaag
     1861 gcgtcgcatt gcgaagcacg agacgacata aagtcggctc gtgacctgct tcgcatgatt
     1921 gtttgcagga tacaagtata tttggcgccg taattccgca cggagagcca gagtgggcaa
     1981 ggacgcaccg gagcacatca gcacgcggat catttcgcag cgcgagctga tcgtcgacgg
     2041 cgcgccgttc caatggtggg ccgtcggcga gcgccccgcg ctcgtgaccg tgcgctctaa
     2101 cgtcttcggc agcctcacga aattcaccga cggagacgtc gcggaatacg ccgcgtcgct
     2161 cgccaaggag ctgctcgcgc agcactacgc gcgcgccggg cggtcgccgg cggggaagcg
     2221 attgccgacc gaagcaacca cgggcttgaa caagccgggc tggttcgagc ccggcgacgc
     2281 cgtcgacttc agcacgacgg tgttctaaag catcaaggtt cgtacccgta gctgcgaatg
     2341 atctcgcgag cttcggcgcc tttgacgaac tcgaggaaca tgcgcgcggc tgggacctca
     2401 cgcagcagca cggcttcttg ctcgatcggc cgatgaagcg attcgggcac gatccagtac
     2461 gacccttcga tttgcttgcc gggttggcgg agctgcgcaa gcgcgacgaa gccgagctcg
     2521 gcgttgcgtg tctgcacgaa cgcgtaggcc tgaccgatgt cttgacccat cgcgagccgg
     2581 ggcatgagcc gctcccagag accgcgggcg gtcagagtct cgcgcgcggc ggcgccgtag
     2641 ggcgcgaggt cggggttcgc gatcgatagt cgccgaaacc ctgccttctc caacactcgt
     2701 ccttgatcgt cgacgtagtt cggctcgggg ctccacagca cgagccggcc gaccgcgtac
     2761 cggaagcgcg agccgggaac ggcgacgcct tcctgctcga gcaggcgcgg gcgctcggca
     2821 tccgccgcga agaacgcctc gaacggtgcg ccgttcttga tttgcgcgta atagttgccc
     2881 gtcgaaccgg agctcacgag cacgtggtgc ttcgtctcga gctcgaagcg ttggacgagg
     2941 tccctcatcg ccgccgtgaa gttcgtggcg accgcgatcg tgatctcgtc cgcgaccgct
     3001 tggcgggaca cccatgatgc gatcaggacc ccaacgcatg cgagccagcg tttgcgcatc
     3061 gtcaacggat accgccaatg aattctgccg cgcaatatac aaccgaatat agcgctatcc
     3121 ccatgtgccc cgggcggtct cgccatagcc atgcgtttcg gcgcatctgc accttgccaa
     3181 accgacggcg ctgtagtagg ttctcctaca gatgtaggag gaaggctttg gccgaaccga
     3241 agctcagcaa gctcgagctc aagatcatgg aagccctgtg ggccaagggg ccgtcctcgg
     3301 tccgcgagat ccaggagagc tttcccgcga agcggcgccc cgcgtacacg accgtgcaaa
     3361 cgatcgtcta ccggctagag gcgaagaacg cgctgaagcg cacgaagaag atcggcaacg
     3421 cgcacatctt cgaggccgtc gtctcgaaga cggccgcgca ccgcaagctc atcgacgacg
     3481 tgctcggcct cttcggcggc cgtgcgcagc cgctaatggc gcatctgatc gaatcgggcc
     3541 agctcacgct cgcggacatc gaggacgcac gcaaggcgct gcgcgacacc gcgaagaagg
     3601 agtgatcgca atgctgaacg agatcgtgaa tcacgtctgg cagtcgacgt tggtcgcggc
     3661 cgcgatcgcg gcgctcgccg cgctgctgcg tggcgactcc gcgcactcgc gctactggct
     3721 ctggtgggcc gcgtcgctga agtttctcgt tccgttctcg ctgctcgcgg cactcggcgc
     3781 cgcgcttcgc gacgccggcg cgccgcgcgt cgagctcgcc gactggcccg cgacgcttgg
     3841 cgccctggca gagccgatgc ccgaggccgc cggctgggcc gcgctgccgg cggcgctcgc
     3901 cgcgatctgg gctaccgggt tcgcggcggt ggccggcgcg tggaccgtgc gcgctctcaa
     3961 ggtgcgcgcg ctgctgcgca gcgcctcgcc gtacgccggc gtgctgccgc gcggcgccgg
     4021 gcgcctcgaa gctcgtacgt cgaccgcgct gctcgagccc gcgctcgtcg gcatcgcgcg
     4081 gcccgtattg ctgttgccgc acggcatcgc ggagcacctg acgcgcagcc agctcgacgc
     4141 cgtgctcgag cacgagctca cgcactggcg ccgccgcgac aatctgacgg ccgcggtcca
     4201 catggtcgtc gaggccgtgt tctggtttta cccgctcgtc tggtggatcg gcgcgcggct
     4261 cgtcgaggag cgcgagcgcg cgtgcgacga ggccgtcgtg cgcgcgggcc acgacggccg
     4321 tgcttacgcc gagggaatac tgaacgtctg cgagcgctac gtcgcgtcgg cgctcaagtg
     4381 tgccgcggga atcagcggcg ccgacctcaa acgacgcgtc gtcgaaatag cgaggaaccg
     4441 tgtcatgagc gaattgccgt ttcagaagaa agcgctgctg ggagccgttg cgctcgccac
     4501 gttgatcgtg ccgatcatct tcggagctgc ggcgcaggac ggcgccgatc cgataccgat
     4561 ccttcgcatc cagccgaatt atccggaaga cgccctcgtc gcggggcgtg aaggatccgt
     4621 ggatctcgag ttcacgattg ccgcgaacgg ctcggtcaag gatgtcgtcg tcgtcagctc
     4681 gaccgcgccg gagttcgagg aacccgcggt caaggcgctg cttcgctggc gcttcttgca
     4741 gaccaacacg acgtgcaccg gaacggactg ccgaacgatc gagaaggtcg agcccgtcga
     4801 gcggcccggc atgcggaccg tcatccatta ccggctctcg gacgcaaggc cggagaccga
     4861 ctgagcggcg cgttcagcgc gaccgctccg ccgcgatcca tgcgtcgacg cggcgctcga
     4921 ggatcgcgag cggcagcgcg ccgccgccga gcacggtgtc gtgaaacctg cggatgtcga
     4981 accgatcgcc gagctctgtc ctcgcgcgct cgcgtagctc gaggatcttc agcatcccga
     5041 tcttgtacgc cgtcgcctgg cccggcgtga cgatgtagcg gcggatctcg gagcggacct
     5101 gcccctccga cgtcgggccg ttcgcgagga agtagtcgac ggcttgctgc tcggtccagc
     5161 ctttcgagtg caggcccgtg tcgacgacga gccggatcgc gcgccagatc tcggcgccga
     5221 gccgcccgaa gtcggagtag gggtcttgat acgcgcccat ttccttcgca agccgctcgg
     5281 catagagacc ccagccttcc gtgtacgcgg tgtagccggc ctgcgtgcgg aacatcggca
     5341 cgccggtcag ctcttgcgcg atcgagatct gcatgtggtg gcccgggatc ccttcgtgat
     5401 aggccacgct ctcgagctcg accttcggca tcgaattcat gtcgatcagg tgcacgtagt
     5461 acgttccggg acgcgagccg tcgggcgtgc cgggctggta gtgctgcgcg gcgcccggct
     5521 gctcgcggaa cgcctcgacg cgcttgacga ccaggccggc cttcggcagc agcccgaagt
     5581 actgcggcaa gcgcgactgg atcacggcgt agtagtcgcg cgacgtttgc aggtaacctt
     5641 cgcgcccttc gtcgctgttc gggaacagaa accgcggatt catgcgcagc gacgcgaaga
     5701 actcctgcag cgtgccgtcg aacccgactt gctgcttgat cgcgttcatc tcggcgtgga
     5761 tccgctcgac ctcggcaaga ccgagctcgt gaatctcgtc ggccgtcatc gtcgtcgtcg
     5821 tgctcgcggc gagccgctcg ttgtaatacg cgggcccgtc gggcagcttc caaacgcccg
     5881 tcgcgacctc gccgctgttc gcgcgatcgg ccgatgccca cgcgatgatg gcgtcgtacg
     5941 cgggcttgac gcgctcggtc aacgtggccg cggctgccgc gcgcagctcg tcggcgcgcg
     6001 cggatgagct ccgggcggca gccgcggccg cgttgaccga gcgcgtcaag cccgcgtacg
     6061 acgacatcat cgcgtgggca tcggccgatc gcgccaacag cggcgagatc gcgacgggcg
     6121 tttggaagct gcccgacggg ccggcgtact acaacgagcg gctcgccgcg agcacgacga
     6181 cgacgatgac ggccgacgag at
//
LOCUS       DASSNN010000799         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1726066, whole genome shotgun sequence.
ACCESSION   DASSNN010000799 DASSNN010000000
VERSION     DASSNN010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726066"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1233)
                     /locus_tag="VFL84_15020"
     CDS             complement(91..1233)
                     /locus_tag="VFL84_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008846406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7609991.1"
                     /translation="MVGGGEGAFIGAVHRAAAGLDQAIELVCGAFASDAERSRRSGAT
                     LGLAPERCYADFASMFAAEAQLPAAKRMEFVAIVTPNDLHLAVATAAFRHGFHVLSDK
                     PATRTLAECIELRDALKASGCLYGLTHPYASYPLVVEAAERVRAGQLGTVRKVLVEYT
                     QGWLAEPIERRGQKQAIWRLDPKRAGLSSCMGDIGVHAFHLAELVTNLRTTELCADLN
                     SIVAGRELDDDGAAFLRFDNGARGVLVASQICTGDENDLRLRVYGDAASLEWRQQEPN
                     SLWLKHAHKPVELIRSGGPGLGAAAHANSRLPSGHPEGYLEAFANVYRHFAAQVRARG
                     GNGNGGPAPRAVPGIAAALRGMVFIETVVAASKSPQKWHAFPNMTS"
     gene            1332..3014
                     /locus_tag="VFL84_15025"
     CDS             1332..3014
                     /locus_tag="VFL84_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007106336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET7609992.1"
                     /translation="MANENYDAIVVGSGISGGWAAKELTEKGLTVLLLERGHNIEHAK
                     DYVNAHKHPWEYPHRGGRTYAMEEAYPVLRRDYPLNEKNVAWWVDEKESPYTEVKRFD
                     WYRGYHVGGRSLLWGRQSYRWSDYDFEANARDGIAVDWPIRYAEIAPWYDYVERHAGI
                     SGSIEGLPQLPDGQFQPAMPLNCGEELVAGRVRGLFDGRRRIIPGRVANLTRPLPGRS
                     PCQYRNACSLGCPYGAYFSTQSSTLPAAMATGRLTLKPFAIATEILYDRDLKRATGVR
                     VLDAVSEQTTEYTARVVFVCASTLNSTWLLMRSATDVWPEGLGSSSGELGHNLMDHHF
                     RTGAEGFLVDEQLNGQYYYGSRPTGFYIPRYRNIGERREYLRGFGYQGSASRRNWQRA
                     VRELGIGGAFKDRAAEPGEWQIGATAFGEILPRHDNKISLDKTRTDKWGLPVLAIDCE
                     TGENERLMREDMKNDMAELLEAAGVRSVTTYDRGYAMGMGIHEMGTARMGNDPRTSVL
                     NRWNQVWDAPNVFVTDGSCMVSSSCVNPSLTYRALTARAVDYAVGELQRRNL"
BASE COUNT          520 a         1056 c         1101 g          502 t
ORIGIN      
        1 gccggccgaa acggcggatc gcgccgcggc tctagccgtg ccgcccgagc ggctctacgc
       61 cgaaccgcgc atcggctacg gctagcgcgc tcagctcgtc atgttcggga acgcgtgcca
      121 cttctgcggc gacttgctcg ccgcgacgac ggtctcgatg aacaccatgc cgcgcagcgc
      181 cgccgcgatg ccgggcacgg cgcgcggcgc agggccaccg ttgccgttgc cgccgcgcgc
      241 acgcacttgt gccgcgaagt ggcgatagac gttcgcgaac gcttcgagat agccctcggg
      301 gtggcccgac ggcaatcgcg agttcgcgtg cgccgccgcg cccaggccgg ggccgccgct
      361 cctgatcagc tcgacgggct tgtgcgcgtg ctttagccag agcgagttcg gctcctgctg
      421 ccgccactcg aggctcgccg cgtcgccgta gacgcgcaaa cgcaggtcgt tctcgtcgcc
      481 cgtgcagatc tggcttgcga cgagcacgcc gcgcgcgccg ttgtcgaagc gcaagaaagc
      541 ggcgccgtcg tcgtcgagct cgcggcccgc gacgatgctg ttcaaatccg cgcagagctc
      601 cgtggtgcgc aggttcgtga cgagctcggc gaggtggaac gcgtgcacgc cgatgtcgcc
      661 catgcagctg cttaggcccg cgcgcttcgg gtcgagccgc cagatcgctt gcttttgtcc
      721 gcgccgctcg atcggctccg cgagccagcc ctgcgtgtac tcgacgagaa ccttgcgcac
      781 ggtgccgagc tgcccggcgc gcacgcgctc ggcggcctcg accacgagcg gatacgaggc
      841 atagggatgc gtgaggccgt agagacagcc gctcgccttc agcgcgtcgc gcagctcgat
      901 gcattcggcg agcgttctcg tcgcgggctt gtcggagagc acgtgaaagc cgtgccggaa
      961 agcggcggtc gcgacggcga gatgcaggtc gttcggcgtg acgatcgcga cgaattccat
     1021 gcgtttcgcc gcgggcagct gcgcttcggc cgcaaacatc gaagcaaaat ccgcgtagca
     1081 gcgctcgggc gctagaccta gggtcgcgcc gctcctgcgg ctgcgctccg cgtcgctcgc
     1141 gaacgcgccg cagacgagct cgatcgcttg atcgagaccg gccgcggcgc gatggacggc
     1201 gccgatgaat gcgccttcgc cgccgccgac catgccgaat ctgacttttc tcacggggcg
     1261 aattctagcg taaggctcga tttcgctcgc cgatgcagta acatccgccc gtttacgcta
     1321 gaggcagcga agtggcgaac gagaattacg atgcaatcgt cgtgggttcc ggcatttcgg
     1381 gcggctgggc cgccaaggag ctcacggaaa agggcctgac ggtgctcttg ctcgagcgcg
     1441 gccacaacat cgagcacgcg aaggactacg tcaacgctca caagcatccg tgggaatatc
     1501 cgcatcgcgg cgggcgcacg tacgcgatgg aggaggcgta cccggtcctg cgccgcgact
     1561 atccgctcaa cgagaaaaac gtcgcgtggt gggtcgacga gaaggagtcg ccgtacacgg
     1621 aggtcaagcg cttcgattgg tatcgcggct accacgtcgg cggccgctcg ctgctgtggg
     1681 gccggcagag ctatcgctgg agcgactacg atttcgaggc caatgcgcgc gacggcatcg
     1741 ccgtcgactg gccgattcgt tacgccgaga tcgcgccttg gtacgactac gtcgagcgcc
     1801 acgccggcat ctcgggctcg atcgaaggcc tgccgcagct tcccgacggc cagtttcagc
     1861 cggcgatgcc gctcaattgc ggcgaggagc tcgtggccgg ccgcgtgcgc ggcttgttcg
     1921 acgggcgccg gcgcatcatt ccgggccgcg tcgcgaacct gacgcggccg ctgccgggcc
     1981 gctcgccgtg ccagtatcgc aacgcgtgct cgctcggctg cccgtacggc gcgtacttca
     2041 gcacgcaatc ctcgacgctg cccgccgcga tggcgacggg gcggctcacg ctgaaaccgt
     2101 tcgcgatcgc gaccgagatc ctctacgacc gcgacctaaa gcgcgcgacg ggcgtgcgcg
     2161 tgctcgacgc ggtgtcggag cagacgaccg aatacacggc acgcgtcgtg ttcgtgtgcg
     2221 cgtcgacgtt gaacagcacg tggctgctca tgcgctcggc aaccgacgtc tggcccgaag
     2281 gcttgggctc gagctcgggc gagctcggcc acaatctcat ggaccatcat ttccgcaccg
     2341 gcgccgaagg cttcttggtc gacgagcagt tgaacggcca gtactactac ggcagccggc
     2401 cgacgggctt ctacattccg cgctatcgaa acatcggcga gcggcgcgag tacctgcgcg
     2461 gattcggcta ccaaggcagt gcgagccgtc ggaactggca gcgcgccgtg cgcgagctcg
     2521 ggatcggcgg cgcgttcaag gaccgcgcgg cggagcccgg ggagtggcag atcggcgcga
     2581 ccgcgttcgg cgagattctg ccgcggcacg acaacaagat ctcgctcgac aagacccgca
     2641 cggacaagtg gggcttaccc gtgctcgcga tcgattgcga gaccggcgag aacgagcgcc
     2701 tcatgcgcga ggacatgaag aacgacatgg ccgagctgct ggaagccgcc ggcgtgcgca
     2761 gcgtcacgac gtacgaccga ggctacgcga tgggcatggg cattcacgag atgggcacgg
     2821 cacggatggg caacgatccg cggacgtcgg tgttgaatcg ctggaatcag gtctgggacg
     2881 cgccgaacgt gttcgtgacg gacggctcgt gcatggtgtc ctcgtcgtgc gtgaacccgt
     2941 cgctgaccta cagggcgctg acggcgcgcg ccgtcgacta cgccgttggc gagctccagc
     3001 ggcgcaacct ctgatcgatg gagaagcacg tggctgcaga tggcaaagac gaactcctgt
     3061 cgcggcgcga agcgattctg cgcgtcagcg cgctcctcgg cggcgtcacg ctgatcggcc
     3121 agagcgcgtg gttggccggc tgcacgacgg cgccgcgctc atcggcgaat ctcttcagc
//
LOCUS       DASSNN010000800         4017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1727836, whole genome shotgun sequence.
ACCESSION   DASSNN010000800 DASSNN010000000
VERSION     DASSNN010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727836"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..790
                     /locus_tag="VFL84_15030"
     CDS             29..790
                     /locus_tag="VFL84_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007236248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7609993.1"
                     /translation="MTLRFISLGSGSRGNASLVEFGSTVLMVDCGLPLKIVEERLQAA
                     DRQPSDIKAILVTHEHGDHAQGVAAFTKRYNTPVFMTCGTASALPAITRVRHLSPHRA
                     LTVGEIDVQPYPVPHDAREPCQFTFGAGGRRLGMLTDAGYVTPHMVERLSECDALALE
                     CNHDLESLQRGPYPESLKARVSSRFGHLNNHQTTELLHRLDATRVQWVLGLHLSERNN
                     SPEHVRATLKPALDNARYPLHLATQDLPTAWLAVE"
     gene            865..>4017
                     /gene="purL"
                     /locus_tag="VFL84_15035"
                     /gene_synonym="purI"
     CDS             865..>4017
                     /gene="purL"
                     /locus_tag="VFL84_15035"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET7609994.1"
                     /translation="MTMLQLAGPPAATAFRLEKLRAELHSLAPTVTDVASHFEHFVHL
                     ERPLTEREDSVLRALLDYGGGAAARTTGRQKLYVVPRLGTISPWASKATDIARICSLP
                     VLRIERGRVFELAVRSVLTDAERQRVAPLLHDRMTETLLDAAPKEEQLFAAHAPRPAR
                     TVDVLAGGRAALERANAELGLALSRDEIDYLAEQFAALKRNPTDVELMMFAQANSEHC
                     RHKIFNADWIIDGERAPKSLFAMIRNTHARSPHGVLSAYKDNAAVLTGAEARWFWPDP
                     ATGVYAYAQEPAHIAIKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKAGLC
                     GFTVSHLEVPGWRQPWERASPGKPDRISSPLAIMLEGPIGAASFNNEFGRPNLAGYFR
                     TALLEANGIWRGYHKPIMIAGGLGNVREANVAKAKPGPGAKLVVLGGPAMLIGLGGGA
                     ASSQGSGAGEAELDFASVQRGNAEMQRRAQEVIDACWALGEENPIAAIHDIGAGGLSN
                     AVPEIVEHSGCGAVIELRAIPNDEPGMSPLELWCNESQERYMLTIEARDVARFAAIAE
                     RERAPYAVIGELTESRELVVRDSSLGGTPVAMPMDVLFGKPPKMTRQAKRTPFKPPEW
                     RRQQLSLASAVDRVLAFPAVADKSFLIHIGDRTVGGLSSRDQLVGPWQVPVSDVAVTA
                     AGYEGYTGEAFAMGERTPVAIHDGPASARLAVAEAVLNIAAADVASLSDVRLSANWMA
                     AAGHADDDYALYAMVRAVGEELCPALGLAVPVGKDSLSMRTVWREDGRERAVTAPVSL
                     IVSAFAPVADVRRTLTPELDRERDSVLVFVDLAGGVQRLGGSALAQTFGQFGGAAPDV
                     DDPQRLKRWFAVQRELRADGLVLAYHDRSDGGLFTALVEMAFAANVGLDVELDAAASD
                     AVAYLFNEELGAVIQVPADGYDRVAQALAAQRLPHHAVARIAKHDDVVVRRAGAELYR
                     APRVELRRKWSELTYRMQALRDDPQCAREAYDAVLDAKDPGLSAKLTFPLPAERRAAA
                     RAGRPQVAVLREQGVNGQREM"
BASE COUNT          615 a         1393 c         1426 g          583 t
ORIGIN      
        1 cactgagcgc tagtcacgga acgcgcgcgt gacgttgcgc ttcatctcac ttggctcggg
       61 cagccgcggc aacgcgtcgc tcgtcgagtt cggctcgacg gtgctcatgg tcgactgcgg
      121 cttgccgctc aagatcgtcg aggagcggct gcaagcagcg gatcggcagc cgagcgacat
      181 caaggcgatt ctcgtcacgc acgagcatgg cgatcatgcg caaggtgtcg cggcgttcac
      241 gaaacgctac aacacaccgg tcttcatgac ttgcgggacc gcgagcgcat tgcccgcgat
      301 cacgcgcgtc cgtcacttga gcccacatcg cgcgctgacg gtcggcgaga tcgacgtgca
      361 gccgtacccg gtgccgcacg atgcgcgcga gccgtgccag ttcacgttcg gcgcgggcgg
      421 ccgccggctc ggaatgctga cggatgcggg ctacgtcacg ccgcacatgg tcgagcggct
      481 gagcgagtgc gacgcgctcg cgctcgagtg caatcacgac ctcgagtcgc tgcagcgcgg
      541 gccgtatccg gagtcgctga aggcgcgcgt atcgtcgcgc ttcggccact tgaacaatca
      601 tcagacgacg gagctcttac accggctcga cgcgacgcgc gtgcagtggg tgctcgggct
      661 gcacttgagc gagcgcaaca actcgcccga gcacgtccgc gcgacgctga agccggcgct
      721 cgacaacgcg cgctacccgc tgcatctcgc cacccaggat ctcccgactg cgtggctcgc
      781 cgtcgagtaa gcgaagtggc ggcttcgaag cgcgcgagag tagaatgctc gtgccgaaaa
      841 gctctcctca cgtgaagtga acccatgacg atgttgcagt tggccggccc gccggcagcg
      901 actgcgttcc gactcgaaaa gctccgtgcc gagctgcact cgctcgcccc gacggtcacc
      961 gacgtcgcaa gccatttcga gcacttcgtt cacctcgaac ggccgctgac cgagcgcgag
     1021 gatagcgtcc tacgggcgtt gctcgactac ggcggcggcg ccgccgcgcg gacgacgggc
     1081 cggcagaagc tctacgtggt cccgcggctc ggcacgatct cgccgtgggc gtcgaaagcc
     1141 accgacatcg cgaggatctg ctcgctgccg gtgcttagga tcgaacgcgg acgcgtgttc
     1201 gagctcgccg tgcgctccgt cctcacggac gcagagcggc aacgcgtcgc gccgctgctg
     1261 cacgaccgca tgacggagac gctgctcgat gcggcgccga aggaggagca gctgttcgcc
     1321 gcgcacgcgc cgcgtcccgc ccgcaccgtc gacgtgctcg cgggcggccg tgcagcgctc
     1381 gagcgcgcca acgccgagct cggcctcgcg ctgtcgcgcg acgagatcga ctacctcgcg
     1441 gagcaattcg cggcgctgaa gcgcaatccg accgacgtcg agctcatgat gttcgcgcag
     1501 gcgaactccg agcactgccg gcataagatc ttcaacgccg actggatcat cgacggagag
     1561 cgcgcgccga agagcctgtt cgcgatgatc cgcaacacgc acgcgcgctc gccgcacggc
     1621 gtgctgtcgg cgtacaagga caacgccgcg gtgctgaccg gcgccgaagc gcgctggttc
     1681 tggcccgatc cggctaccgg cgtgtacgcg tatgcacaag agccggcgca catcgcgatc
     1741 aaggtcgaga cgcacaatca tccgacggcg atctcgccgt tcccgggcgc ggcaacgggc
     1801 tcgggcggcg aaatccgcga cgagggcgcg acgggccgcg gcgccaagcc gaaggcgggc
     1861 ttgtgcggct tcacggtctc tcacctcgag gtgccgggct ggcggcaacc gtgggagcgc
     1921 gcgtcgccgg gcaagccgga ccgcatctcg tcgccgctcg cgatcatgct cgagggcccc
     1981 atcggcgcgg cgagcttcaa caacgagttc ggccggccga atctcgccgg ctacttccgc
     2041 acggcgttgc tcgaggcgaa cggcatttgg cgcggttatc acaagccgat catgatcgcc
     2101 gggggcctcg gcaacgtgcg cgaggcgaac gtcgctaagg ccaagcccgg tcccggcgcg
     2161 aagctcgtcg tgctcggcgg ccccgcgatg ctgatcgggc tcggcggcgg cgccgcgtcg
     2221 tcgcaaggca gcggcgccgg cgaggccgag ctcgacttcg cctcggtgca gcgcggcaac
     2281 gccgagatgc agcggcgcgc gcaagaggtc atcgacgcgt gctgggcgct cggcgaagag
     2341 aacccgatcg cggcgattca cgacatcggc gccggcgggc tttcgaatgc cgtgcccgag
     2401 atcgtcgagc atagcggctg cggcgccgtc atcgagctgc gcgcgatccc gaacgacgag
     2461 cccggcatga gcccgctcga gctctggtgc aacgagtcgc aagagcggta catgctcacc
     2521 atcgaagcgc gcgacgtggc gcgctttgcg gcgatcgccg agcgcgagcg cgcgccgtac
     2581 gccgtgatcg gcgagctcac ggaaagtcgc gagctcgtcg tgcgcgactc gtcgctcggc
     2641 ggcacgccgg tcgcgatgcc gatggacgtg ctgttcggca agccgccgaa gatgacgcgc
     2701 caggcgaagc gcacgccgtt caagccgccg gaatggcggc gccagcagtt gagcctcgcg
     2761 tctgccgtcg accgcgtgct cgcgttcccg gccgtcgcgg acaagtcgtt cctgatccac
     2821 atcggcgacc gcaccgtcgg gggcttgagc tcgcgtgacc agctcgtcgg tccatggcaa
     2881 gtacccgtca gcgacgtcgc cgtcacggcg gccggttacg aaggctacac cggcgaggcg
     2941 ttcgcgatgg gggagcgcac gccggtcgcg attcacgacg gtcccgcgtc ggcgcggctc
     3001 gcggtcgcag aggccgtgct gaacatcgcg gccgccgacg tcgcgtcgct gtcggacgtt
     3061 cgcctgtcgg cgaactggat ggcggccgcg ggccacgccg acgacgatta cgcgctgtat
     3121 gcgatggtgc gcgccgtcgg cgaggagctc tgcccggcgc tggggctcgc ggtccccgtc
     3181 ggtaaggact ccttatctat gcgcacggtc tggcgcgagg acggccggga gcgggccgtc
     3241 acggcgcccg tgtcgttgat cgtctcggcg ttcgcgcccg tcgccgacgt ccgccgcacg
     3301 ctgacgcccg agctcgatcg cgagcgcgac tccgtgctcg tgttcgtcga tctcgcgggc
     3361 ggagtgcagc gcctcggcgg ctcggcgctc gcgcaaacgt tcggccagtt cggcggcgcg
     3421 gcgcccgacg tcgacgatcc gcagaggctg aaacgctggt tcgccgtgca gcgcgagctg
     3481 cgcgcggacg gcctcgtgct cgcgtatcac gaccgctccg acgggggcct gttcacggcg
     3541 ctcgtcgaga tggcgttcgc ggcgaacgtc ggtctcgacg tcgagctcga cgcggccgcg
     3601 agcgacgccg tcgcgtatct cttcaacgag gagctcggcg ccgtgatcca ggtgccggcc
     3661 gatggctacg accgcgtcgc gcaggcgctc gcggcgcaac gcctgccgca tcacgccgtc
     3721 gcgcgcatcg cgaagcacga cgacgtcgtc gtgcggcggg caggtgcaga gctctatcgc
     3781 gcgccgcgcg tcgagctgcg ccgcaagtgg tccgagctga cgtacaggat gcaggcgctg
     3841 cgcgacgacc cgcagtgcgc gcgcgaggct tacgacgccg tgctcgacgc gaaagatccg
     3901 ggcttgagcg cgaagctgac gttcccgctg cccgcggagc ggcgcgcggc cgcgcgtgcc
     3961 ggcaggccgc aggtcgcggt gctgcgcgag cagggcgtca acggccagcg cgagatg
//
LOCUS       DASSNN010000801         4415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1007129, whole genome shotgun sequence.
ACCESSION   DASSNN010000801 DASSNN010000000
VERSION     DASSNN010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007129"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2475)
                     /locus_tag="VFL84_15040"
     CDS             complement(<1..2475)
                     /locus_tag="VFL84_15040"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7609995.1"
                     /translation="MIRAVIALLVAVAAGAAYADEHIADIRQGTNMSVALSPGGTTLV
                     VDLLERLWSLPATGGGAVPLTKEGERARNPRFSLDGGRVVYQQRSGTQWDLWLLDMAT
                     GEQRPLTTSAFDEREPDFTADGRAVVFASNRTGHDCLWSITLDGGVETQLTEEAGAAS
                     YPTVSEHGLVAYVLARDDEWSIRVLGLDGAIDVVHTSASRLSPPSWRPGGGVLVFGEQ
                     DSAETSRLQLLLLGEPRVLKSLSGSEDLFAARAAWRSGAEFLYTADGQLWRRGLATPT
                     REPVHLNAAALVETAAPPSALAPLDDAAERTAFGVNGLVRSPDGRRWAFTALGDVWVA
                     DRGEPRRLTDDPFVDLDPSFWPDGDSLVVASERTGQFELWRIDVREGRATQLTFGALQ
                     PRRPVVRPDGNAVAYLESAGLEPNAATVVKMLDLRRREDGAVATNVIGASALAWAEDG
                     RTLIVRARSADPVAGRDLRVDLGAAAPQPEETAAQAPPLVHWRAPAAPPDYVVEVGRL
                     FDGAGDTYRRHVDVHVRGGRIAAIVARGALPAPGPIVDARDATVIPGLIDLHAHQSAL
                     VGERLGRAWLAFGVTTVRELTPAAAEAVERAEAWASGRSPGPRLLVSPLHGTEASSSP
                     AVRAYPGIAQGLAHSLRRQARDIAIPPWELAALPARLRYDADAPTLELELSPGFVAYQ
                     DSVSRLIVSETTFVTGLAALAGMQGWPAPKPRRDAAYALFTPVEQAVWERPDALGAAI
                     PALERTIARLVRAGGRVGVGSDAPAVPYGLGLHLELALLSEAGIANDQVLRMATAEGA
                     LALGLEREVGTIEEGKLADFVVIDGDP"
     gene            complement(2472..2660)
                     /locus_tag="VFL84_15045"
     CDS             complement(2472..2660)
                     /locus_tag="VFL84_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HET7609996.1"
                     /translation="MIQFETIACPYCWEPIEIALDLSVESQQQVEDCSICCRPIVVRY
                     STADGELAALEVAAENRE"
     gene            complement(2662..3297)
                     /gene="pdxH"
                     /locus_tag="VFL84_15050"
     CDS             complement(2662..3297)
                     /gene="pdxH"
                     /locus_tag="VFL84_15050"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET7609997.1"
                     /translation="MGSLLIPPPEDPVRTASQWLGEAEALGRRNPNAMALASVDALGR
                     PSVRMVLLKELAPLGYAVFYTNYGSRKGTELLATGRAAAVLYWHELGRQVRFEGPVVR
                     SPEAESDAYFATRPTPSQINAWSSEQSRPLPDPAALEERAAQVASTKPAGSGLPRPDF
                     WGGFRLWLEAIELWVEGRDRFHERVRYERRLDARDAYTFETGRWNWQRLQP"
     gene            complement(3304..4149)
                     /gene="fghA"
                     /locus_tag="VFL84_15055"
     CDS             complement(3304..4149)
                     /gene="fghA"
                     /locus_tag="VFL84_15055"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HET7609998.1"
                     /translation="MTTPSIEQVSESRCFDGRQIVYKHASSACACTMRFAVYLPPAAE
                     RGAVPALYWLSGLTCTEENFSVKAGAQRYAAELGLALIIPDTSPRGVNIPGEDAQMDV
                     GTGAGFYVNATEPPWAAHYRMYDYIRDELPAVVNGNLPVDPARASISGHSMGGHGAII
                     LGFRNPQKYRSVSAFAPIASASKSAWGQHALTAYLGADQSRWSEYDAAAVIRAKPSRH
                     ELLVDQGTADPYLAQLLPNELKEACRASGQRLTYRERAGYDHGYYFVSTFIGDHLRFH
                     AAALR"
     gene            4231..>4415
                     /locus_tag="VFL84_15060"
     CDS             4231..>4415
                     /locus_tag="VFL84_15060"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET7609999.1"
                     /translation="MTLEELRKRLSEVDRELIGLVAARQKIVAEIGSHKIQNSVPTRD
                     YEREREVLKGASTQARA"
BASE COUNT          680 a         1538 c         1549 g          648 t
ORIGIN      
        1 cgggtcgccg tcgatgacga cgaagtcggc gagcttgcct tcctcgatcg taccaacctc
       61 gcgctcgagc ccaagcgcaa gcgcgccttc cgccgtggcc atgcgcagga cctgatcgtt
      121 ggcgatcccg gcctccgaaa gcagcgcgag ctcgagatgc aggccgagac cgtaaggcac
      181 ggccggcgcg tcggatccga cgccgacgcg gccgccggca cggacgagcc gcgcgatcgt
      241 ccgctcgagg gccggaatcg ccgcgccgag cgcgtcgggc cgctcccaga cggcttgctc
      301 gacgggggtg aacagcgcgt acgcggcgtc gcggcgcggc ttcggcgcgg gccaaccttg
      361 catcccggcg agcgccgcga ggccggtcac gaacgtggtc tcggaaacga tgagccggct
      421 cacgctgtcc tggtacgcca cgaagccggg ggaaagctcg agctcgagcg tcggcgcatc
      481 ggcgtcgtag cgaagacgcg ccggaagcgc tgcgagctcc cagggcggta tcgcgatgtc
      541 gcgggcttgc cggcgcaggc tatgtgccaa gccttgcgcg atgccggggt acgcgcgaac
      601 ggcggggctc gagctcgcct ccgtgccgtg gagcggcgaa acgagcaagc gcgggcccgg
      661 cgagcggccg ctcgcccagg cttcggcgcg ctcgacggct tccgcggccg cgggcgtcag
      721 ctcgcgcacc gtcgtgacgc cgaaggcgag ccaggcccgc ccgaggcgtt cgccgaccag
      781 cgcgctttga tgcgcgtgca gatcgatgag ccccgggatc acggtcgcgt cgcgtgcgtc
      841 gacgattggc cccggcgcgg gcagcgcgcc gcgcgcgacg atcgccgcga tgcggccgcc
      901 ccgcacgtgc acgtcgacgt gccggcgata cgtatcgcct gcgccgtcga acagccggcc
      961 gacctcgacg acgtagtcgg gcggcgcagc cggagcgcgc caatgaacga gcggcggcgc
     1021 ctgcgccgcc gtttcctcgg gctgcggcgc cgccgcgccg agatcgacgc ggaggtcgcg
     1081 gcctgcgacc gggtcggccg acctagcccg cacgatcaga gtgcggccgt cctcggccca
     1141 tgcgagcgcg ctggcgccga tgacgttggt cgcgacggcg ccatcctcgc ggcgtcgcaa
     1201 gtcgagcatc ttgacgaccg tcgcggcatt cggctcgagg cccgcgctct cgaggtacgc
     1261 cacggcgttg ccgtcgggcc gcacgacggg ccggcgcggc tgcagcgcac cgaacgtcag
     1321 ctgcgtggca cgcccctcgc ggacgtcgat tcgccacagc tcgaactgac ccgtgcgctc
     1381 gctcgcgacg accaacgagt ccccgtccgg ccagaagctc gggtcgaggt cgacgaaggg
     1441 atcgtccgtg agccggcgcg gctcgccgcg gtcggcgacc cacacatcgc cgagcgccgt
     1501 gaacgcccaa cgccggccgt cgggcgagcg aacgaggccg ttcacgccga atgccgtccg
     1561 ttcggccgca tcgtcgagcg gcgcgagagc gctcggcggt gcggcggtct cgacaagggc
     1621 ggcggcgttg agatgcacgg gctcgcgcgt cggcgtcgcg agtccgcgcc gccagagttg
     1681 cccgtcggcg gtatagagga attcggcgcc cgagcgccag gccgcgcgcg ccgcgaacag
     1741 gtcctcgctg ccgctcaacg acttcagcac gcgcggctcg ccgagaagca gaagctgtag
     1801 acgactcgtc tccgcggagt cttgctcgcc gaagaccagc acgccgccgc cgggccgcca
     1861 cgaaggcggc gacaaacggc ttgcgctcgt gtgaacgacg tcgattgcgc cgtcgagacc
     1921 gagcacgcgg atcgaccact cgtcgtcgcg ggcgagaacg tacgcgacga gcccgtgctc
     1981 cgacacggtc ggatacgatg cggcaccggc ttcctccgtc aattgcgtct cgacgccgcc
     2041 gtcgagcgtg atcgaccaaa ggcagtcgtg tcccgtgcga ttgctcgcga agacgacggc
     2101 gcgcccgtcg gccgtgaagt cgggctcgcg ttcgtcgaag gccgacgtcg tcagcggccg
     2161 ttgctcgccc gtggccatgt cgagcagcca gaggtcccac tgcgtgccgc tgcgctgttg
     2221 atagacgact cgcccgccat ccaagctgaa gcgcggattc cgtgcccgct cgccttcctt
     2281 ggtcaaaggg acggcgccgc cgcccgtcgc ggggaggctc cacagccgct ccaacaggtc
     2341 gacgacgagc gtcgtgccgc ccggcgacaa ggcgaccgac atgttcgtgc cctggcggat
     2401 gtcggcgatg tgctcgtcgg cgtaagcggc gcccgccgcg acggcgacca gaagcgcgat
     2461 gaccgcccgg atcactcgcg gttctccgcc gcgacctcga gcgcggcgag ctcgccgtcc
     2521 gccgtgctgt aacgaacgac gatcggtctg cagcagatgg agcagtcttc gacttgctgc
     2581 tgcgactcca ccgatagatc gagcgcgatc tcgatcggct cccagcaata aggacacgcg
     2641 atcgtctcga actgaatcac gctagggttg caggcgctgc caattccagc ggcccgtctc
     2701 gaaggtgtaa gcgtcgcgcg cgtcgagccg gcgctcgtag cggacgcgtt cgtggaagcg
     2761 atctcggccc tcgacccaga gctcgatcgc ctcgagccac aagcggaagc cgccccaaaa
     2821 atcgggccgg ggcaggccgc ttccggcagg cttcgtgctc gcgacttgcg cagcgcgttc
     2881 ctcgagcgcc gccgggtcgg gcagcggccg gctctgctcg ctgctccagg cgttgatttg
     2941 gctcggcgtc ggccgcgtcg cgaaatacgc gtcgctctcg gcctccggcg agcgaacgac
     3001 cgggccttcg aagcgcacct gcctgccgag ctcgtgccaa tagagcacgg ccgccgctct
     3061 gcccgtcgca aggagctcgg tgcccttgcg cgagccgtag ttcgtgtaga acacggcgta
     3121 accgagcggc gcgagctctt tcagcaagac catgcgcacc gacggccggc ctagcgcgtc
     3181 gacgctcgcg agcgccatcg cgttcgggtt gcgtcgcccc aaggcttcgg cttcgccgag
     3241 ccactgcgag gcagtgcgca cgggatcctc gggcggaggt atcagcagcg accccatggc
     3301 ggctcagcgc agcgccgccg catggaaccg caagtggtcc ccgatgaacg tgctgacgaa
     3361 gtagtagcca tggtcgtagc ccgcgcgctc gcgataagtc aggcgctgac ccgaggctcg
     3421 acacgcctct ttcagctcgt tcggcagcag ctgcgctaga tacggatcgg ccgttccctg
     3481 gtcgacgagc agctcgtgcc ggctcggctt ggcgcggatg acggcggccg cgtcgtactc
     3541 gctccaacga ctttggtcag cgcccaggta agcggtcaac gcgtgttggc cccacgcgga
     3601 cttgctggcc gaggcgatcg gcgcgaacgc cgagacggag cgatacttct gcgggttgcg
     3661 gaatcctaag atgatggcgc cgtggccgcc catcgagtgg ccgctgatcg acgcgcgcgc
     3721 cgggtcgacg ggcagattcc cgttgacgac tgcgggcaac tcgtcgcgga tgtagtcgta
     3781 catgcggtag tgcgctgccc acggcggctc ggtcgcgttc acgtagaagc cggcgccggt
     3841 gccgacgtcc atctgcgcgt cctcacccgg gatgttgacg ccgcgtgggc tcgtgtcggg
     3901 aatgatcagc gcgagcccga gctcggcggc atagcgctgc gcgccggcct tgacgctgaa
     3961 attttcttcc gtgcaggtga gccccgacag ccaatagagg gccgggaccg cgccgcgctc
     4021 cgcggccggc ggcaggtaca cggcgaagcg catcgtgcag gcgcacgccg acgaggcgtg
     4081 cttgtagacg atctggcggc cgtcgaaaca acgcgattcg ctgacctgtt cgatggacgg
     4141 tgtggtcatt cactttcctt cagtctgagc atcagtcggc ttgcgcttcc gaccccaggg
     4201 gcgttacaac cgcgcctcac agaggtaggc atgactctcg aagaattacg taaacgtctc
     4261 tccgaggtcg atcgcgagct gatcggtctc gtcgccgccc ggcagaagat cgtcgccgag
     4321 atcggatccc acaaaattca gaattcggtg ccgacgcgcg attatgagcg ggagcgcgag
     4381 gtgttgaaag gcgcctcgac ccaagcgcgt gcctt
//
LOCUS       DASSNN010000802         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1007735, whole genome shotgun sequence.
ACCESSION   DASSNN010000802 DASSNN010000000
VERSION     DASSNN010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007735"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VFL84_15065"
     CDS             <1..1137
                     /locus_tag="VFL84_15065"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HET7610000.1"
                     /translation="ERLRAAVADACRRWSFDSRRWVLTLSGGADSRSLLCLLRHRGIG
                     TVTWGVPESAEQDGNDAQVARQITRALAVPHRFFAIDSSADAPEVVLERFLAAGEGRV
                     ARISGYVDGFRIWKTLFDEGYDGVIRGDEAFGSLSVSTQQGVRCMASLTTLDDYFSKE
                     ECEGFELPPQHLPEWLLQGRGETLATWRDRLYQQFRVPTLLAGLTDLKTAYVEVGSPL
                     LASSVLACVRALPDDLRTEKSLWHELVGAQLPNLALATRVAIPSLTDALSDRRVLSLM
                     LDELSSERAGSVFAPLLRARCCSALRSALRGSGSARRENRGYPLLSRAVPGRLRAVVR
                     NWRSGRPAVEPLVLAFRAFVAARMQRILRSDAATAAGLKPAVNA"
     gene            1158..>2285
                     /locus_tag="VFL84_15070"
     CDS             1158..>2285
                     /locus_tag="VFL84_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610001.1"
                     /translation="MVRAILVAPRHPNRPHRLDAYCDLLNRKLAGDNIEPRAPTLVRR
                     DGLALALLNPNSAVHTHGASFGIGMLLGARDDWHVPRAPVPDGSFALLRVDHAYVELV
                     ADSVASRTLWYALTDDELIVSSSQRAIVTLLGSYEPNREVLPWMLSSGTLGPAGGWDA
                     RLKRVQPGERMLLDRARWRAQSTFEPVALVPDRRLSAAAHLERLGAAVDNACSRWSFD
                     PRKWVLTLSGGSDSRSLLCLMRDRGIGTVTWGLPNTGEQDGNDAQVAREVARSLNVPN
                     RFLALEPHGESPDVIVERFIAVGEGRVDRLSGYIDGFRVWKTLFDDGYHGVIRGDQAF
                     SKALVRSEYAARSGTNLATLADYFSAEELATFELPEQRIPAR"
BASE COUNT          322 a          789 c          801 g          373 t
ORIGIN      
        1 gagcggttgc gcgcggcggt cgcggacgcg tgcaggcgct ggtcgttcga ttcgcggaga
       61 tgggtgttga cgttgtccgg cggcgcggac agccgcagcc tgctgtgcct gctgcgccac
      121 cgcggcatcg gaaccgtgac ctggggagtg cccgaaagcg ccgagcagga cggcaacgat
      181 gcgcaagtag cgcggcagat cacgcgcgcg ctggccgtcc cgcaccggtt cttcgcgatc
      241 gactcgagcg ccgatgcgcc cgaggtagtg ctcgagcggt tcctcgcggc cggcgagggc
      301 cgagttgcgc gcatctcggg ctacgtcgac ggattccgca tctggaagac gctgttcgac
      361 gaaggctacg acggcgtcat ccgcggcgac gaagcgttcg gctccctgtc ggtcagcacg
      421 cagcaaggcg tgcgctgcat ggcgagcctg acgaccctcg acgactactt ctcgaaagag
      481 gaatgcgagg gcttcgagct tccgccgcag cacctgcccg agtggctgct gcaaggccgc
      541 ggcgaaacgc tcgcgacctg gcgcgatcgt ctataccagc agtttcgagt accgacgctg
      601 ctggccggcc tgaccgatct caaaaccgcc tatgtcgagg tcggcagccc gctgctcgcg
      661 agctccgtgc tcgcgtgcgt gcgcgcgttg cccgacgatc tgcgcaccga aaagagtctc
      721 tggcacgagc tcgtgggcgc ccagctgccg aacttggcgc tcgccacgcg cgtcgcgatc
      781 ccgtcgttga cggacgctct atccgaccgc cgcgtgctct cgctgatgct cgacgagctg
      841 tcctccgagc gcgccggaag cgtgttcgcg ccgcttctgc gcgcgcgctg ttgcagcgcg
      901 ttgcgctcgg cgttgcgcgg ctcgggttcc gcgcgccgtg aaaaccgcgg ctaccccttg
      961 ctgtcgcggg ccgtgcccgg tcggctgcgg gccgtcgtcc gcaactggcg gagcggccgc
     1021 ccggccgtgg agccgctcgt cctcgcgttt cgagcgttcg tcgccgcgcg catgcagcgg
     1081 attctgcgct cggacgccgc aactgcggcc gggctcaagc cggccgtcaa cgcctgagac
     1141 gacggcgcgg agcattcatg gttagagcca tcctcgtcgc gccgcgccat ccaaaccggc
     1201 cgcaccgcct cgacgcgtat tgcgacctgc tgaaccgcaa gctcgccggc gacaacatcg
     1261 agccgcgcgc gccgacgttg gttcgccggg acggcctcgc gctcgcgctg ctgaatccca
     1321 acagcgccgt gcacacgcac ggcgccagct tcgggatcgg catgttgctc ggcgcccgcg
     1381 acgactggca cgtgccgcgc gcgccggtgc cggacgggag cttcgcgctg ctgcgcgtcg
     1441 atcatgccta cgtcgagctc gttgccgaca gcgtggcgtc gcgaacgctc tggtacgcgc
     1501 tgacggacga cgagctgatc gtctcgagct cgcagcgcgc gatcgtgacg ctgctcggct
     1561 cgtacgagcc gaatcgtgaa gtcttgccgt ggatgctgtc gtcgggaacg ctcggcccgg
     1621 ccggcggctg ggacgcgcgt ctgaaacgcg tgcagccggg tgagcgcatg ttgctcgatc
     1681 gagcgcgctg gagagcgcag tcgacgttcg agccggtcgc gctcgtcccc gaccggcgac
     1741 tctcggcggc cgcgcacctc gaacgtctcg gcgcggcggt cgacaatgcg tgcagccgct
     1801 ggtctttcga tccgcgaaaa tgggtgctga cgctgtccgg cggctccgac agccgcagct
     1861 tgctgtgcct gatgcgcgac cgcggcatcg gaaccgtcac atggggcctg ccgaacacgg
     1921 gcgagcagga cggcaatgac gcgcaggtcg cgcgcgaggt cgcgcgctcg ttgaacgtgc
     1981 cgaatcgctt tctggcgctc gagccgcacg gggagtcgcc cgacgtcatc gtcgagcggt
     2041 tcatcgcggt aggcgaaggc cgcgtcgaca ggctttccgg atacatcgac ggctttcgcg
     2101 tttggaagac tctgttcgac gacggctacc acggcgtgat ccgcggcgac caagcgttca
     2161 gcaaggcgct cgtgcgctcc gagtatgccg cgcgctccgg gacgaacctg gcgacgctcg
     2221 ccgattattt ctcggccgag gagctcgcca cgttcgagct gccggagcaa cggatccccg
     2281 cgcgg
//
LOCUS       DASSNN010000803         4540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1348543, whole genome shotgun sequence.
ACCESSION   DASSNN010000803 DASSNN010000000
VERSION     DASSNN010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348543"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFL84_15075"
     CDS             complement(<1..858)
                     /locus_tag="VFL84_15075"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7610002.1"
                     /translation="MERAQAGSSALWIGDWLVEPTLARVSRAGEVQRVTPRAMAVLLC
                     LAEAKGTVVSRNDILDAVWPGLAVTPDALSQCLVELRRVFGDSPKQPGVIETIPKMGV
                     RLVAPVVQKRSPPAQRDGEPPARAEGERRSLAVLPFDNLSADPSNAYVAAGMQEEILT
                     RLSRLANRFRVISRTSVLRYAAERPSIREIAQELGADIILEGSVRSARDKLRVDVRLV
                     DAATDSVVWSDSYDGQRDDALSMQSAVSLDVARALRVELSAAEKASLDRALTNSTRAY
                     ELYAQANYLY"
     gene            1058..1465
                     /locus_tag="VFL84_15080"
     CDS             1058..1465
                     /locus_tag="VFL84_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610003.1"
                     /translation="MTAPACSCPKPANSRGRAFVLALLCAMVTLPPAFAGSLGNVTPD
                     RWQPHSDVGVLERVMLRVHWYDSLELLREAAVGNDMSGEGLHGFSVLRRNTATGEWVC
                     DVYVVKMRGALVDNDRTVTFGHEVLHCFGLRHQ"
     gene            complement(1512..1886)
                     /locus_tag="VFL84_15085"
     CDS             complement(1512..1886)
                     /locus_tag="VFL84_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7610004.1"
                     /translation="MKLHGVRYQVKDVGRAVDFYTRHLGFNVDRTHLPAFGQVSIGDF
                     KLILSGPGASGSRPMPDGHEQQPGGWNRVILEVDDLAARIDTLRKAGLTFRNRLEAGP
                     GGKQIQIEDPDGNPVELFEPAH"
     gene            2009..3172
                     /locus_tag="VFL84_15090"
     CDS             2009..3172
                     /locus_tag="VFL84_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HET7610005.1"
                     /translation="MRRFCSLFSQILNFIPRTDFQRRVRELNAERHARGVSCWQQFVA
                     MLFCQLGRAHSLREIEQGLKSCEGKLSHLGIEPVGRSSLSYMNEHRPWELYEKVFFDL
                     LGYCQSQGNWRHKFRFKNKLMSLDSTVIDLCVSMYDWAKFRRTKGAVKLHLLLDHDGY
                     LPAFAVITTGKVHDVRVARSLKLQPGTIIVDDRGYTDYELFGSWTDEGVYFVTRMRDN
                     AVYEVIEARPVPSCHPHIQSDQVIRLTGKNAEQKCPHQLRRIEVRDEEQPRTLVFLTN
                     NPQLSAATIAAIYKDRWSVELFFKAIKQNLKLKTFVGTSPNAVKIQIWTALIAMLILR
                     FLQLKSKFGWSLSNLVALLRMNLFTYRDLWAWLDEPFATPPDHSGPEQVPLAL"
     gene            complement(3312..3872)
                     /locus_tag="VFL84_15095"
     CDS             complement(3312..3872)
                     /locus_tag="VFL84_15095"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET7610006.1"
                     /translation="MTRAQCTALQTQELTDYWLGELDEVREQRVDEHVFACGACSDRL
                     RTLVDLGAAIRGAFVDGWLNIVLPEAFIRRVKEAGFQVREYDLAAGGSVNCTVTPDDD
                     FVVAHLRAPLRGVKRLDVLIDDDTSGKHRAADVAFDPSAGELVTVTSTAYLKTLKHSQ
                     QRVRLVAVDGVDERVVADYTFNHYPS"
     gene            complement(3869..4453)
                     /locus_tag="VFL84_15100"
     CDS             complement(3869..4453)
                     /locus_tag="VFL84_15100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7610007.1"
                     /translation="MGFGAYEADDDAALARRIIAAAPSRRDAAAEAELCRRLGPRIRL
                     YGRKHLRSEAAAADLMQDVLILLLDKLREGAVRDPSQVVSFVLGTARQTVVDWRRSGA
                     RRTRLLEKFPLDLQPSESSDGGPEPIDAERLRGCLGALPERERAVLVMTFYDDRPADA
                     VASELGLSAGNVRVIRHRGLERLRGCMAATEGAA"
BASE COUNT          788 a         1500 c         1458 g          794 t
ORIGIN      
        1 gtacaagtag ttcgcctggg cgtagagctc gtatgcgcgc gtcgagttgg tcaacgcgcg
       61 atcgaggctc gccttctcgg cggccgagag ctctacgcgc agagcgcgcg cgacgtccag
      121 cgagaccgcg gactgcatcg agagcgcgtc atcgcgctgc ccgtcgtaac tatcgctcca
      181 gacgacgctg tcggtcgccg catcgacgag ccgcacgtcg acgcgcagct tgtcgcgcgc
      241 gctgcgcacg ctgccctcga gaatgatgtc cgcgccgagc tcttgcgcga tctcgcggat
      301 cgagggccgt tccgccgcat agcgcagcac cgaggtgcgc gagatcacgc ggaagcgatt
      361 cgcgaggcgc gagagtctcg tcaggatttc ctcctgcatg cccgcggcga cgtacgcatt
      421 gctcgggtcc gcactcaagt tgtcgaacgg cagcacggcg aggctacgcc gctctccttc
      481 ggcgcgggcc ggcggctcgc cgtcgcgctg cgcgggcggc gagcgttttt gcacgacggg
      541 tgcgacgagc cgcacgccca tcttcggaat cgtttcgatg actcccggct gcttcgggct
      601 gtcgccgaag acgcggcgca gctcgacgag gcattgcgac agcgcgtccg gcgtcacggc
      661 caggcccggc catacggcgt cgaggatatc gttgcgcgag acgaccgttc ctttggcctc
      721 ggcgagacag agaagcaccg ccatcgcgcg cggcgtgacg cgctgaacct caccggcccg
      781 cgacacgcgc gcgagcgtgg gctcgacgag ccaatcgccg atccaaagag ccgagcttcc
      841 cgcttgcgcg cgctccattc ccactccgac ggcacgtttc cgagcttccc gcgggcgtga
      901 gtctagcgcc tgccggccga accgggctaa ccgctgctcg tgctccgctc gtgcgttgct
      961 cacgcgcccg tcggccgggc atcgcgctca gctccggatt ccgcgcggct cgatcattaa
     1021 aaaagagctc catcgacgga cttccatgga gcgccacatg accgcacccg cttgttcctg
     1081 ccccaagccc gccaacagcc gagggcgcgc cttcgtgctc gcgctgctat gcgcgatggt
     1141 cacgctaccc cctgccttcg ccggttcttt gggcaacgtg acgccggatc gctggcagcc
     1201 gcactcggac gtcggagttc tcgagcgcgt catgctgcgc gtgcactggt acgacagtct
     1261 cgagctgctg cgcgaggccg cggtcggaaa cgacatgagc ggcgaaggcc tgcacggctt
     1321 ctcggtcctt cgccgcaaca cggcgaccgg cgaatgggtc tgcgacgtct acgtcgtcaa
     1381 gatgcgcggc gcgctcgtcg acaacgaccg aaccgtcacg tttgggcacg aggtgctgca
     1441 ctgcttcggc ttgcgccacc aatgagccgc gcggcgggtc gcgccgcgaa gccggctgcg
     1501 cgaaccgctc gctaatgcgc cggctcgaac agctcgacgg gattgccgtc ggggtcctcg
     1561 atctggattt gcttgccgcc cgggcccgct tccaatcgat tgcgaaaggt caagcccgct
     1621 tttcggagcg tgtcgatacg agcggcgaga tcgtcgacct cgaggatcac gcggttccaa
     1681 ccgccgggct gctgctcgtg accgtcgggc atcggccgcg accccgaagc gcccggaccg
     1741 ctcaagatca gcttgaagtc gccgatcgag acctgtccga acgccggcag gtgcgttcgg
     1801 tccacgttga aaccgaggtg ccgtgtgtag aagtcgacgg cacgtccgac gtctttgacc
     1861 tgataacgga ctccatgcaa tttcatcact cttgtccaaa ttagctcggt gatttggagc
     1921 gatttttcac gggaaagacg ttgagcctcc tcaggtaagc tggattggca accaaaccga
     1981 tcctgcttta cagcaaagga gactcaacgt gaggcgattc tgtagcctgt tcagccagat
     2041 tctcaacttc attcctcgaa ccgacttcca acggcgggtc agggaactga acgcggagcg
     2101 acacgctcgt ggggtgagct gctggcagca gttcgtggcg atgctgttct gtcagctcgg
     2161 acgcgcccat agcctgcggg agatcgagca ggggctgaag tcctgcgaag gcaagctcag
     2221 ccatctgggg atcgagccgg tcgggcgctc gtcgctgtct tatatgaacg agcatcgtcc
     2281 ctgggagctg tacgagaagg tgtttttcga cttgctcggc tactgccaaa gtcaaggcaa
     2341 ctggcggcac aagttccgct tcaagaacaa gctgatgagc ctggactcca ccgtcatcga
     2401 cctgtgcgtg tcgatgtacg actgggcgaa gttccgccgc accaagggag cggtgaagct
     2461 gcatctgcta ctcgatcacg acggctacct gcccgccttt gccgtcatca ccaccggcaa
     2521 ggtgcatgac gtgcgggtgg cgcggagcct gaaattacag cctggcacga tcatcgtcga
     2581 tgaccgcggc tacaccgatt acgagctgtt tggcagctgg acggatgagg gggtctattt
     2641 cgtcacccgt atgagggaca acgcagtcta cgaggtcatc gaggcgcgcc ccgtcccgag
     2701 ctgccacccc cacatccaaa gcgaccaagt gattcggctc acagggaaga acgccgagca
     2761 gaaatgcccg catcagctgc gacggatcga agtccgggac gaagagcagc cgcgaacgct
     2821 ggttttcctc accaacaacc cccagctctc ggccgctacc atcgccgcca tctacaagga
     2881 tcgctggtcg gtggagttgt tcttcaaggc gattaagcaa aacctcaagc tcaaaacctt
     2941 cgtcggcact tcgccgaacg cggtcaagat ccagatctgg acagcgctca tcgccatgct
     3001 gatcctgcgt ttcttgcagc tgaagtcgaa gtttggctgg tcgctgtcga accttgttgc
     3061 gctgctgcgc atgaacttgt tcacgtatcg cgatctgtgg gcctggttag acgagccgtt
     3121 tgccacaccg ccggaccaca gcggccctga acaggttccg ctggctctct aattcggaca
     3181 gcatcaaggg tgggcttcaa cctcgatcgt ccgcagagtc cgtccaaata cgcaaggaag
     3241 acgatattcg tgctggtcaa ttcgagctaa tttggacagc agtgatctgt ccccttttag
     3301 cgcttttagc gctaagacgg gtagtgattg aacgtgtagt cggcgacgac gcgctcgtcg
     3361 acgccgtcca cggcgacgag ccgaacgcgt tgctgtgaat gcttcagcgt cttcaagtac
     3421 gccgtgctcg tcaccgtcac gagctcgccg gccgagggat cgaacgcgac gtcggccgcg
     3481 cgatgcttgc cgctcgtgtc gtcgtcgatc agcacgtcga gccgcttcac gccgcgcagc
     3541 ggcgcgcgca gatgcgcgac gacgaagtcg tcgtcgggcg tgacggtgca attcacgctg
     3601 ccgccggccg cgaggtcgta ctcgcgcacc tggaagcccg cctctttgac gcggcgaatg
     3661 aacgcctcgg gcagcacgat gttgagccag ccgtccacga acgcgccgcg gatcgcggcg
     3721 cccagatcga cgagcgtgcg caggcggtcg ctgcacgcgc cgcacgcgaa cacgtgctcg
     3781 tcgacgcgct gctcgcgcac ttcgtcgagc tcgccgagcc agtagtcggt gagctcctgc
     3841 gtctgcaaag cggtgcattg cgcgcgcgtc atgcggcacc ctcggttgcg gccatgcagc
     3901 cgcgcaatcg ctcgagaccg cgatggcgga tcacgcgcac gttgccggcg gagagcccga
     3961 gctcggaagc gacggcgtct gcgggccggt cgtcgtagaa cgtcatgacg agcacggcgc
     4021 gctcgcgctc gggcagcgcg ccgaggcagc cgcgcaggcg ctcggcgtcg atcggctccg
     4081 ggccgccgtc ggacgattcg gacggctgca gatcgagcgg aaatttctcg agcagccgcg
     4141 tgcgccgcgc gccgctgcgc cgccaatcga ccacggtctg gcgcgccgtg ccgagcacga
     4201 acgacacgac ctgcgacggg tcgcgcacgg ccccctcgcg cagcttgtcg agcagcagga
     4261 tcagcacgtc ctgcatcagg tccgcggccg cggcttcgct gcgcaggtgc ttccgcccat
     4321 agagccggat acgcgggccc aatctccggc agagctcggc ctcggcggcg gcgtcgcgcc
     4381 gcgacggcgc agcggcgatg atgcgccgcg cgagcgcggc gtcgtcgtcc gcttcatagg
     4441 ccccgaatcc catgcggaca ggatacgggc ggacagtagc cagcgaaaac atgcacggaa
     4501 gtgtcgcgag ccgcggcctc gttacaggcg gtgcccgtta
//
LOCUS       DASSNN010000804         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1729576, whole genome shotgun sequence.
ACCESSION   DASSNN010000804 DASSNN010000000
VERSION     DASSNN010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729576"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFL84_15105"
     CDS             complement(<1..579)
                     /locus_tag="VFL84_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610008.1"
                     /translation="MSTEPDRDPALAALWREHSTETPSPRVDAAILAAAHSAVANAPH
                     GRERSPGQRAWRWWVPLAAAAVVAVVVVGVKPPSQTIVDDAAQSASDMPAASAEKQSA
                     TPTPAAPPAAPPVAPLAMTSPRTEAAQPSIAPPPQVAPAAPDTSASRGRGKVDAERVQ
                     AFAAPPPARAAASIAAPAQPASPAGAAAGGSNS"
     gene            complement(569..1234)
                     /locus_tag="VFL84_15110"
     CDS             complement(569..1234)
                     /locus_tag="VFL84_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7610009.1"
                     /translation="MALVGGIGSGVAAATSATHTARMSDAPAEDDEKFMLAYARGEAA
                     AFDALYARHRNGVYRYLLRHCNNAGIADELFQDVWMNVIRSRATYAPTARFATWLYTL
                     AHHRIVDHWRSTGQAKLVSTDADETAGAEILALAGSRGDQPEVRAFTGQIRERLNAAL
                     AALPPAQRDAFLLQQESGLSLAEIAALTGVGIETVKSRLRYAAARLRAALIELREASA
                     DEH"
     gene            complement(1248..2027)
                     /locus_tag="VFL84_15115"
     CDS             complement(1248..2027)
                     /locus_tag="VFL84_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610010.1"
                     /translation="MTCAAAQPALAHWPARPLASVDVYDRTSGSVLAVYEKDGQRYVV
                     GTPGHEYEIRIRNRTGERILAVTSVDGVNVVSGETASPAQSGYVIDANGGVEIAGWRR
                     SLERTSAFYFTDLGDSYAARTGRPKDVGVIGVAVFRERPRANALSSLRDKLAANEDAR
                     RERGDVPVSPGPSAAAEGTTQARPLAAAPKLGTGFGRDETSYAERVRFERASESPVET
                     IAIQYDRRDNLIAMGVLPQPRYAQRTPDPFPAMQFVPAPPR"
     gene            2310..2504
                     /locus_tag="VFL84_15120"
     CDS             2310..2504
                     /locus_tag="VFL84_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HET7610011.1"
                     /translation="MRTNIVIDDRLMKEVLAATGLGTKREAVELGLRTLLRLYRQAEI
                     RRYRGKLPWTGDLEVMRLDT"
     gene            2501..2902
                     /locus_tag="VFL84_15125"
     CDS             2501..2902
                     /locus_tag="VFL84_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019092622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HET7610012.1"
                     /translation="MILVDSSVWIDYFRGAATPEADTLDALLGTEPLATGDLILTEVL
                     QGFAADADFARARKLLMSLTIVHLGGVGIAVKAAQNFRRLRARGVSVRKTIDTVIATY
                     CIENRLALLHSDRDFDPFVNHLGLQTVARLA"
BASE COUNT          446 a         1003 c          990 g          482 t
ORIGIN      
        1 tgagttcgaa ccccctgccg cggctccggc aggcgatgcc ggttgcgccg gcgccgcgat
       61 cgacgcagca gcacgcgcag gtggcggcgc cgcgaacgct tgcacacgct cggcgtcgac
      121 cttccccctt ccacgcgacg cgcttgtgtc cggcgcggca ggcgcaacct gcggcggtgg
      181 cgcgatgctc ggctgagcag cttcggttcg ggggctcgtc atagcgagcg gtgcaacagg
      241 tggcgcagcg ggtggcgcag cgggtgtcgg cgtggcactt tgcttctctg ccgacgccgc
      301 cggcatgtcg cttgccgact gcgccgcgtc atcgacgatc gtttgcgacg gcggcttgac
      361 gccgacgacg accaccgcga ccaccgcggc tgcggccagc ggcacccacc agcgccaggc
      421 ccgctgaccg ggcgatcgtt cgcgcccatg cggtgcattc gcgacggcgc tatgcgcggc
      481 cgcgagaatc gccgcgtcga cgcgcggaga cggcgtctcg gtcgagtgct cgcgccacag
      541 ggcggcgagc gccgggtcgc gatcgggttc agtgctcatc ggcggatgct tctcgcaatt
      601 cgatcaacgc ggcgcgcagg cgcgcggccg cgtaacggag gcggctcttg accgtctcga
      661 tccccacacc cgtcagtgcc gcgatttcgg cgagtgaaag cccgctttcc tgctgcaaca
      721 ggaatgcgtc gcgctgcgcc ggcggcagtg ccgcaagcgc cgcgttcagc cgctcgcgaa
      781 tctggccggt gaaggcgcgc acttccggct gatcgccacg cgatcccgcg agcgccagga
      841 tttccgcacc ggcggtctcg tccgcgtcgg tcgacacgag cttcgcctgt cccgtcgacc
      901 gccagtgatc gacgatgcgg tggtgcgcca gcgtgtacag ccacgtcgcg aagcgcgccg
      961 tcggcgcata agtcgcgcgc gaccggatca cgttcatcca gacgtcctgg aacaactcgt
     1021 ccgcgattcc ggcgttattg cagtggcgaa gcagatagcg atacacgcca ttgcggtggc
     1081 gcgcgtagag cgcgtcgaac gccgccgcct cgccgcgcgc gtaggcgagc atgaatttct
     1141 cgtcatcctc ggcgggcgca tcgctcatgc gcgcagtatg ggtagccgac gtcgccgccg
     1201 caacgccact gccaatgccg ccgaccaacg ccacgagcga gaacgcttca gcgcggcggg
     1261 gccggcacga attgcatggc aggaaacgga tcaggcgtcc gttgcgcgta gcgcggctgc
     1321 gggagcacgc ccatcgcgat caggttgtcg cgacggtcgt actggatcgc gatcgtctcg
     1381 acgggcgatt cgctcgcgcg ctcgaagcgc acgcgctccg cgtaggacgt ctcgtcgcga
     1441 ccgaagccgg tgccgagctt gggtgccgcc gcgagcggcc tcgcttgcgt ggtgccctcc
     1501 gcggctgcgg aaggaccggg cgacaccggc acgtcaccgc gctcgcgacg cgcatcttca
     1561 ttcgcggcga gcttgtcgcg caacgacgac agcgcattcg cgcgcggccg ttcgcgaaac
     1621 acggcgacgc cgatcacgcc gacgtccttc ggccggccgg tgcgtgccgc atagctgtca
     1681 cccagatcgg tgaagtagaa cgccgaggtc cgctcgagac tgcgccgcca gccggcgatt
     1741 tcgacgccac cgttcgcgtc gatgacatag cccgactgcg cgggcgacgc cgtctcgccg
     1801 ctcaccacgt tgacgccgtc gacgctcgtc acggcgagga tgcgctcgcc cgtccggttc
     1861 cggatgcgga tctcgtactc gtggccgggc gtgccgacga cgtagcgctg gccgtccttc
     1921 tcgtagaccg ccagcacgct gccgctggtg cggtcgtaga cgtcgacgct ggcgagcggg
     1981 cgcgccggcc agtgcgcaag cgccggttgc gcggcggcgc acgtcattgc tgcgagcaga
     2041 agcagccttg ccggccaccg accgcgcttg ccacgattgc ggcgtcccga tgcgttcatg
     2101 gagtcctccc cttcaccggt gcgatcgtcc ggtgctgcta gccatgaacg tgcgcgcggc
     2161 gcaaacgggg ttagccggga tcggccgccg agtttcggcc ggatggagga tcgcgaacgg
     2221 cgaccgatgg ctgcattctg ccaatcgatc ttgcccgcgg ccccggacag gagaataatg
     2281 tgtacgacta tcaaaattca tacacatcga tgcgcacgaa catcgtcatc gacgaccggc
     2341 tcatgaaaga agttctcgca gcgactgggc ttggcaccaa gcgcgaggcc gtcgaactgg
     2401 gccttcgcac gctgcttcgc ctctaccggc aggccgaaat ccgccgctac cgcgggaagc
     2461 tgccgtggac gggcgacctc gaagtcatgc gcctcgacac gtgatactcg tcgattcgag
     2521 cgtatggatc gactactttc gcggtgcggc tacgccggaa gccgacacgc tcgatgcgct
     2581 gctcgggact gagccgcttg cgaccgggga tctgatcctg accgaggtgc tgcaaggctt
     2641 cgcggccgat gctgatttcg cgcgggcgcg caagctgctc atgtcgctga cgatcgtaca
     2701 tctcggaggt gttggcatcg cggtcaaggc tgctcagaac tttcggcgat tgcgcgcgcg
     2761 cggcgtcagt gtgcgcaaaa cgatcgatac ggtcatcgcc acctactgca tcgaaaaccg
     2821 gctggcgctg ctccatagcg accgggactt cgatcccttc gtgaaccatc tcgggttgca
     2881 gacggttgcg cgtctcgcgt aggcgtcccg cttgattgct c
//
LOCUS       DASSNN010000805         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1732673, whole genome shotgun sequence.
ACCESSION   DASSNN010000805 DASSNN010000000
VERSION     DASSNN010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732673"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFL84_15130"
     CDS             complement(<1..797)
                     /locus_tag="VFL84_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610013.1"
                     /translation="MRQVLKKTAILAAASLLTTAAAAQQIANTGFKSVGRGWPLAADL
                     RDFETTGPAIPVSFADGAVVRGSWDQFIGSAHHGAAPKGVEPLPVDLFTSKDFYKDRE
                     LWKDPRYFRCNSPAGIEEAWGANTTATIGDNPPASAAWGYCDRDYPREAIVSPYPFKT
                     AQEHYEALLAETTKRGGAHQRPTPKQLEEWTGRYAHPGNTTDAQADWTGKNKSSNPQS
                     YWYRMRHSQVPTILSLLTPEYQTRMVQEAYHAGNTNAPQWPSQYCWP"
     gene            951..1409
                     /locus_tag="VFL84_15135"
     CDS             951..1409
                     /locus_tag="VFL84_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610014.1"
                     /translation="MKLPDDIRKQFARYGRRGGKARAARLHADGRRAIARRAASSRWI
                     RERFGAASFTELGLPGGDVVDTGLADLAAGTVSASSLAVSLAAPRLRREGVPVPIEAV
                     HPNPEDRLYDLLAATAGDLAHARYAACLEQIVSFADACPLARRQRASRAK"
     gene            1399..1932
                     /locus_tag="VFL84_15140"
     CDS             1399..1932
                     /locus_tag="VFL84_15140"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HET7610015.1"
                     /translation="MRNRLTRESLQSLMRELARSARPKQSYRVYLVGGGTAVLEGWRD
                     SSIDADLYAAQDDIFRDVQAIKERLRLNIELARPEDFVPPLEGTDTRHVFIETVGGVS
                     FYHYDPYAQLLSKIVRGFARDLTDARALVTSGMVDPRRFAELVAAIPDTVYSKYPRLS
                     PQAVHAAVEGFLASLPG"
     gene            complement(1970..>2678)
                     /locus_tag="VFL84_15145"
     CDS             complement(1970..>2678)
                     /locus_tag="VFL84_15145"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7610016.1"
                     /translation="ELLGGNWRPGQSAFGQIGEQTVAEAEIVGVIPDLKQGSVQDRVQ
                     PAVYLPHEQLTMRKMAIVVRSETDDPAALIPAIRRELAAMDSTIPSTFAVYSDVVAAS
                     LARDRLGAAGLGVFGLGSWILAAVGTYGLMSFSVNQRFNEIAVRSAFGAERGRLLKMF
                     VTRAAELAGIGIVLGVAGAVAMRQLVASQLYETSALDPWVLALVPLTMLAVTLLASYM
                     PARRASRIDVTAALREN"
BASE COUNT          434 a          904 c          884 g          456 t
ORIGIN      
        1 tccggccagc agtactgcga aggccactgc ggcgcattcg tgttgcccgc gtggtaagcc
       61 tcctggacca tgcgcgtctg atattcgggc gtcagcaacg acaggatcgt cggcacttgg
      121 ctgtgccgca tgcggtacca atacgactgc gggttcgagc tcttgttctt tcccgtccaa
      181 tccgcttgcg cgtcggtcgt gttgccggga tgcgcgtagc ggccggtcca ctcctcgagc
      241 tgcttaggcg taggccgctg gtgcgcgccg ccgcgcttcg tcgtctcggc gagcagcgcc
      301 tcgtagtgct cctgcgccgt cttgaatgga tacgggctca cgatcgcctc gcgcggatag
      361 tcgcggtcgc agtaacccca cgcggcggaa gccggcgggt tgtcgccgat cgtcgccgtc
      421 gtgttcgcgc cccacgcctc ttcgattccg gccgggctgt tgcagcggaa atagcgcgga
      481 tccttccaga gctcgcggtc cttgtagaag tccttcgacg tgaacaggtc aacgggcagc
      541 ggctcgacgc ccttcggcgc agcgccgtga tgagcggagc cgatgaactg gtcccacgag
      601 ccgcgcacga cggcgccgtc cgcgaagctg acggggatcg ccgggcccgt ggtctcgaaa
      661 tcgcgcaagt ccgcggcgag gggccagccg cggccgacgc tcttgaagcc ggtattggcg
      721 atttgctgcg ccgccgcggc cgtcgtcagc aggctggccg cagccaaaat cgcagtcttt
      781 ttaaggactt gacgcatcta atcccccagt cacttatgcg aatcttaatt tgaaaggcgc
      841 ctcaagggcg agttacgagg acttaagaat ccccgatcgc ctcaagccgc ggaagcaaaa
      901 acccgcggcg tggtttgtga tgcttacccc ctatggatat cctagcgaac atgaaactgc
      961 ccgacgacat ccgcaagcag tttgcccgct atggccgccg cggcggcaaa gcccgcgcgg
     1021 ctcgcttgca cgccgacggg cggcgcgcca ttgcacggcg cgcggcctcc tcgcgttgga
     1081 ttcgcgagcg cttcggcgcc gcgagcttca cggagctcgg gctgcccggc ggcgacgtgg
     1141 tcgacacggg gcttgccgac ctcgccgctg gaacggtcag tgcatcgagc ctcgccgtgt
     1201 cgctggcagc gccacggctg cggcgcgagg gcgtgccggt gccgatcgag gcggtccacc
     1261 cgaatccgga ggatcggctc tacgacctgc tggccgcgac agccggcgat cttgcgcacg
     1321 cacgctacgc cgcctgtctg gagcagatcg tctcgttcgc ggacgcgtgc cccctggctc
     1381 ggcgacagcg agcgtcgcgt gcgaaataga ctcacgcgcg aatccttgca gtccctgatg
     1441 cgcgagctcg cgcgttcggc gcggccgaag caaagctacc gtgtctactt ggtcggcggc
     1501 ggcacggccg tgctcgaggg ttggcgcgat tcgtcgatcg atgccgatct ctatgccgcg
     1561 caagacgaca tctttcgcga cgtgcaggcc atcaaagagc gtctgcgact aaacatcgag
     1621 ctcgcgaggc ccgaggactt cgtgccccca ctcgaaggta ccgacacgcg acatgtgttc
     1681 atcgagacgg tcggcggcgt cagcttctat cactacgatc cgtacgcgca gctcttgtcg
     1741 aagatcgtgc gcgggttcgc gcgcgacttg accgacgccc gggcgctcgt gacgagcggc
     1801 atggtcgatc cgcggcgctt cgccgagctc gtggcagcca tcccggatac ggtgtactcg
     1861 aagtatccgc ggctctcccc gcaagccgtg cacgcagccg tcgagggctt cctggcctcc
     1921 ctgcccggct aacgctcggt cgctcatcag cgagttggca atcgaaagtt cagttctcgc
     1981 gcagtgcggc ggtgacgtcg atgcgcgagg ctcgccgcgc cggcatgtac gacgcgagca
     2041 gcgtcaccgc gagcatcgtc agcggcacga gcgcgagcac ccacgggtcg agcgcgctcg
     2101 tctcgtagag ctggctcgcg acgagctggc gcatcgcgac cgcgccggct acacccaaga
     2161 cgatgccgat gcccgcgagc tccgctgcgc gtgtcacgaa catcttgagt agccggccgc
     2221 gctcggcgcc gaatgccgac cgcacggcga tctcgttgaa ccgctggttg accgagaacg
     2281 acatcaagcc gtaggtaccg accgcggcca atatccacga cccgaggccg aacacgccga
     2341 gcccggcggc accgagccgg tctcgcgcga gcgaagcggc gacgacgtcg gagtacacgg
     2401 caaaggtgct cggaatcgtc gaatccatcg ccgcgagctc gcggcgaatc gccggaatca
     2461 gcgcggcggg atcgtcggtt tccgagcgca cgacgatcgc catcttgcgc atggtcaatt
     2521 gctcgtgcgg cagataaacc gccggctgca cgcggtcctg aacgctgccc tgcttgaggt
     2581 cgggaatcac gccgacgatc tcggcctcgg cgacggtttg ctcgccgatc tgaccgaagg
     2641 cggactggcc gggacgccag ttgccgccga ggagctct
//
LOCUS       DASSNN010000806         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1734670, whole genome shotgun sequence.
ACCESSION   DASSNN010000806 DASSNN010000000
VERSION     DASSNN010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734670"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFL84_15150"
     CDS             <1..654
                     /locus_tag="VFL84_15150"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HET7610017.1"
                     /translation="AAPPDPFVGGISLGYLATKGNTDSTNANAKFDVTWDLDGPWKHD
                     WNALAINARTNGVTTAESYSAGYKARRDFSETSYLFFTGDWRQDRFSGYDRQVSEAVG
                     YGRRLIDSERHMLALEGGAGAKQSDLTTGEQLDEAIIRGALDYLFTISETSEFEQKLL
                     IEQGDENRYTESTSELKTRIVGNIALVLSYVVKSNSDVPVGIEQTDRFTSIALEYGF"
     gene            844..1890
                     /locus_tag="VFL84_15155"
     CDS             844..1890
                     /locus_tag="VFL84_15155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7610018.1"
                     /translation="MTKSSAVALALALGASACTDSTPQAPAETTPAAAAIPVVTRPAR
                     VEPMGIEIEAVGTTQANESVEVTSKASNTVTAIRFREGDEVERGAVLVEMDAAQARAA
                     LAEAEAALTRSKSAFDRSRDLQSRQALSVADLEQVEADLKADEARVAAARARLDDTVI
                     RAAFHGRTGFRHVSVGSFVSPGTLITTLDDTSIIKLDFTVPETYLFVLRRGLPVEASA
                     TGLPDRTFTGVITNMDSRVDPVTRSVTVRAELENPDGLLRQGMFMTVSLQGEVSPTLL
                     VPEEALVPERGKAYVFVVRDNVVERREVRTGKRKPGYVEIVDGVHEHEHVVVDGTQHV
                     RDGSKVEETHGGAS"
BASE COUNT          356 a          673 c          665 g          316 t
ORIGIN      
        1 gccgcgccgc ccgatccgtt cgtcggcggc atctcgctcg gctacctggc gacgaagggc
       61 aacaccgaca gcacgaacgc caacgcgaag ttcgacgtga cctgggatct cgacggtccg
      121 tggaagcacg actggaacgc gctcgcgatc aacgcgcgca cgaacggcgt cacgacggcc
      181 gaatcgtact cggccggcta caaggcgcgg cgcgacttct cggagacgag ctatctgttc
      241 ttcaccggcg attggcgcca ggaccggttc tcgggctacg acaggcaggt gtccgaagcc
      301 gtcggctatg gccgcaggct catcgacagc gagaggcaca tgctcgcgct cgaaggcggc
      361 gccggcgcca agcagtcgga tctgacgacg ggcgaacagc tcgacgaggc gatcatccgc
      421 ggcgcgctcg attatctatt cacgatcagc gagacgtcgg agttcgagca gaagctcctg
      481 atcgagcaag gcgacgagaa ccgctacacc gaatcgacgt ccgagctgaa gacgcgcatc
      541 gtcggcaaca tcgcgctcgt gctctcgtat gtcgtcaaga gcaattcgga cgtgccggtc
      601 ggcatcgagc agacggatcg gttcacgtcg atagcgctcg agtacggctt ctagaccgga
      661 gccagtccaa agttttcttg cgctcggcgc tcgcgcaggg ggtagcccga aggcgccgca
      721 gtctccatcg gaaccgtgtg atataaatcg attttccgcg acggttcgga cccatccctc
      781 atgtcatgca tggaacgcgt gcacccgcgc aatgtcggct cgcgccgtag gcggattgct
      841 gcgttgacca aatcgagcgc agtcgcgttg gcgctggcgc taggcgccag cgcgtgcacc
      901 gactccacgc cgcaggcccc cgccgagacc acgcccgcgg cggccgccat tcccgtcgtc
      961 acgcggcccg cacgtgtcga gccgatgggc atcgagatcg aagcggtcgg cacgacccag
     1021 gccaacgaat cggtcgaggt cacctcgaaa gcctcgaaca cggttaccgc gatccgcttc
     1081 cgcgaaggcg acgaggtcga gcgcggggcc gtgctcgtcg agatggacgc cgctcaggcg
     1141 cgagccgctc tcgccgaggc cgaagcggca ttgacgcgca gcaagagcgc gttcgatcgc
     1201 agccgcgatc tgcagtcgcg gcaagcgctc tcggtcgccg atctcgagca ggtagaggcg
     1261 gacttgaaag cagacgaagc gcgcgtcgcg gccgcgcgcg cgcggctcga cgacaccgtg
     1321 atccgcgccg ctttccatgg caggaccggt ttccgccatg tcagcgtcgg cagcttcgtg
     1381 agccccggca cgctgatcac gacgctcgac gatacgtcga tcatcaagct cgatttcacg
     1441 gtccccgaga cctatctgtt cgtgctgcgc cgcgggttgc ccgtcgaggc gtcggcgacg
     1501 gggttgccgg accgcacgtt cactggcgtc atcacgaaca tggattcgcg cgtcgacccc
     1561 gtcacgcgct ccgtcaccgt gcgcgccgag ctcgaaaacc ccgacggcct gctgcgccaa
     1621 ggcatgttca tgacggtctc gctgcagggc gaggtctccc ccacgctgct cgtgcccgaa
     1681 gaggcgctcg tcccggagcg cggtaaggcg tacgtgttcg tcgtgcgcga caacgtcgtc
     1741 gagcggcgcg aggtgcgcac gggcaagcgc aagccgggct acgtcgagat cgtcgacggc
     1801 gtacacgagc acgagcacgt cgtcgtcgac ggcacgcagc acgtccgcga cggcagcaag
     1861 gtcgaagaga cgcacggcgg cgcgagctga tcgatgcgga tctccgagtt gtcggtccgc
     1921 cggccggtct tcgcgaccgt gctcagcatg atgctcgtga tcttcgggct cgtgtcgctg
     1981 cagcgactgt ccgtccgcga gtatcccgat
//
LOCUS       DASSNN010000807         4237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1734885, whole genome shotgun sequence.
ACCESSION   DASSNN010000807 DASSNN010000000
VERSION     DASSNN010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734885"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2070)
                     /locus_tag="VFL84_15160"
     CDS             complement(<1..2070)
                     /locus_tag="VFL84_15160"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7610019.1"
                     /translation="MAATLALGTVLAAGLPLVAGADEDGGSAPARDLGTVTVIGRRPA
                     SLPTQIPTTIEGIDAREIGLRINATDAEDALKYFPSLVVRKRYIGDYDHAVLATRASG
                     TNNSARSLVYVDGILISNLLGNGAAFTPRWGLVTPEEIERVDVLYGPFSAAYPGNSVG
                     AVVDYVTRMPVELEIEAHVSSFGEDFSIYRSQGRYSGSQASASVGNRQGPWSWWLNFD
                     RLDSDAHPVSYANKLVSSGVADSSGTPVTGALAARNPREQDWLILGSTTATHTVQDHA
                     KAKVAYEISPHLIASYTLGWWDNDAQRSSETYLRDAAGNPVYSGDITVDGLRYTVAPA
                     DFAPTNGELRHLMQGLSLKTERQGVWNFDLAASSYEYDRDVVRSPTVALPAAAGGGAG
                     RIAAQDGTGWSTLAVRAARHPESPRGHVLDVGYQRDEYRLQTLVSNADDWLAGGPTAR
                     FSEFRGRTELESMYAQDTWAFAEPWRATFGARVEQWRASNGAIGDATTTIGLPERTEI
                     YVSPKLAIARQLTAEWSLKASLGRAIRFPTVAELYQGSIATNAVVDNDPNLRPERSWT
                     SELTGERTLGTGSIRLTAFFENTEDALYAQTNVTVTPSVTNIQNVDEIRTSGLEVAFR
                     SEPLLGDWLDVAASVTYAHSRIEENANFPASVGKWQPRVPDWRANAFATYRFGDSWSL
                     TAGGRYSG"
     gene            2238..3479
                     /locus_tag="VFL84_15165"
     CDS             2238..3479
                     /locus_tag="VFL84_15165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7610020.1"
                     /translation="MRLLFWLRIVAIAAQVAAVALANFVLAGPLPLRELGLAIGALAL
                     WNVLSYGPVHAARNVHDGEVVLHLAVDIVALTAVLYFTGGATNPFVSLYLVPISLAAT
                     SLPTRYAWLTVAICGAGYAFLWWDSVPLPPVHPRFGSEFDLHLAGMWVNFVIAAVLIV
                     LFVGRMAWQVRQRDRELAAMRENALLDQQVIELGTLAAGTAHELNTPLSTLAILASEL
                     DETTTDAAQKRQLRTMLEEIRVINERLNRIAGGVNAERSAGARHVELDAFLEELLAQW
                     AAAHREIELGVAFATQRNNVAVVVEATIEQAIRNVLDNAAHATLANGGRRIDVSIRVV
                     GTRLELAVKDQGAGLDPAVRDDIGLKAVSTKEHGLGIGMLLSRAALQRFGGRLELNEA
                     PRGGVEARIELPLDELLADAR"
     gene            3469..4020
                     /locus_tag="VFL84_15170"
     CDS             3469..4020
                     /locus_tag="VFL84_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7610021.1"
                     /translation="MPADARRGVLVVDDDARFAVTLAAALARRGYVAHVAHDAAAALA
                     TARAKEPDAAVVDLKLGADDGLALIEPLRAAHPDMRIVVLTGYASIATAVKAIKLGAD
                     DYLAKPVTASAVAAVLERGTRKPAEKRSAEPLETMSPRRLEWEHIQRVLAEHDGNISA
                     TARTLRMHRRTLQRKLLKRPTQG"
BASE COUNT          638 a         1473 c         1454 g          672 t
ORIGIN      
        1 gccgctgtag cggccgcccg ccgtcagcga ccagctgtcg ccgaagcggt aggtcgcgaa
       61 agcgttcgcg cgccaatcgg gcacgcgcgg ctgccacttg ccgacgctcg cgggaaaatt
      121 cgcgttctcc tcgatgcgcg agtgagcgta ggtcacgctc gcagccacgt cgagccagtc
      181 gccgagcaag ggctccgacc gaaacgcgac ctcgagaccg ctcgtcctga tctcgtcgac
      241 gttctggatg ttagtaacgc tcggcgtgac cgtgacgttc gtctgcgcgt acagggcatc
      301 ctcggtgttc tcgaagaacg cagtgagccg gatgctgccc gtgccgagcg tgcgctcgcc
      361 ggtcagctcg gacgtccacg acctctcggg gcgcagattc gggtcgttgt cgacgacggc
      421 gttcgtcgcg atagatcctt gatagagctc ggccacggtc ggaaagcgaa tcgcgcggcc
      481 gagcgacgcc ttgaggctcc actcggccgt gagctgcctc gcgatcgcga gcttcggcga
      541 cacgtagatc tccgtgcgct cgggcagccc gatcgtcgtc gtcgcatcgc cgatcgcgcc
      601 gttcgaggcg cgccattgct cgacgcgcgc gccgaacgtc gcgcgccacg gctctgcgaa
      661 cgcccacgtg tcttgcgcgt acatgctctc gagctccgta cgccctcgga actccgagaa
      721 ccgcgccgtc gggccgccgg cgagccagtc gtcggcgttc gagacgagcg tttgcaagcg
      781 gtactcgtcc cgctgatacc cgacgtcgag cacatggccg cgcggcgact ccgggtgccg
      841 cgctgcgcgc accgcaagcg tgctccagcc cgtgccgtct tgcgccgcga tgcggccggc
      901 gccgccgccg gccgcggcgg gcagcgcgac ggtcggcgag cgcacgacgt cgcggtcgta
      961 ttcgtacgag ctcgccgcga ggtcgaaatt ccagacgccc tggcgctcgg tcttcagcga
     1021 caagccctgc atgaggtggc gcagctcgcc gttcgtcggc gcgaaatcgg ccggcgcgac
     1081 ggtgtaacgc aagccgtcga cggtgatgtc tcccgagtac acggggttgc ccgccgcgtc
     1141 gcgcagatac gtttccgacg agcgctgcgc gtcgttgtcc caccagccga gcgtgtagct
     1201 cgcgatcagg tgcggcgaaa tctcgtaagc gaccttggct ttcgcatggt cctgaaccgt
     1261 gtgcgtcgcc gtcgtcgagc cgagtatcag ccaatcctgc tcgcgcggat ttcgcgccgc
     1321 caacgcgccg gtcacgggcg tgcctgagct atccgcgacg ccgctcgaca cgagcttgtt
     1381 cgcgtagctg accggatgcg cgtcgctgtc cagccgatcg aagttgagcc accaggacca
     1441 aggaccctgg cggttgccga cggaggcgct ggcttggctc cccgagtacc tgccttgcga
     1501 gcggtaaatc gagaagtctt cgccgaagct cgacacgtgc gcttcgatct cgagctcaac
     1561 gggcattcgc gtcacataat cgacgaccgc gccgaccgag ttgccggggt acgcggccga
     1621 gaacggcccg tagagaacgt cgacgcgctc gatctcctcg ggcgtcacga ggccccaccg
     1681 cggcgtgaac gcggcgccgt tgccgagcaa gttcgagatc aggatgccgt cgacgtagac
     1741 gagcgatcga gcgctgttgt tggtgcccga cgcgcgcgtc gcgagcaccg catggtcgta
     1801 atcgccgatg tagcgtttgc gcacgacgag gctcgggaaa tacttcagcg cgtcctcggc
     1861 gtcggtcgcg ttgatgcgca agccgatctc gcgcgcatcg atgccttcga tcgtcgtcgg
     1921 gatctgcgtc ggcaatgagg ccggccggcg gccgatcacg gtgacggtcc cgaggtcgcg
     1981 cgccggcgcg ctgccgccat cctcgtcggc gccggcgacg agcggcaacc ccgcggcgag
     2041 caccgtcccg agcgccaacg tcgctgccac tccgcgtctg tcgtcgaacc tcacacttga
     2101 aattgtaggt agcggcaaac ggcgcggcct gcgaccgatt gtcgcagttg gggggtgggc
     2161 tgtgtgcgaa aatacctagc gtgtcgcagc ccgtcaccaa gctctcgcta gtcgacagcg
     2221 ccggaccgag cggagccttg cggcttttgt tctggctgcg catcgtcgcg atcgccgcgc
     2281 aggtcgcggc cgtcgcgctc gcgaatttcg tgcttgccgg cccgctgccg ctgcgcgagc
     2341 tgggcctcgc catcggcgcg ctcgcgctct ggaacgtgct gagctacggc cccgttcacg
     2401 ctgcacgcaa cgtgcacgac ggtgaggtcg tgctgcacct cgccgtcgac atcgtcgccc
     2461 tgacggcggt gctgtatttc acgggcggcg ccacgaatcc gttcgtctcg ctgtacttgg
     2521 tgccgatctc gctcgcggcg acgtcgctgc cgacgcgcta tgcctggctc accgtcgcca
     2581 tctgcggcgc ggggtatgcg ttcctgtggt gggacagcgt gccgctgccg ccggtccatc
     2641 cgcgcttcgg cagcgagttc gacctgcatc tcgccggcat gtgggtcaac ttcgtgatcg
     2701 ccgcggtgct gatcgtgctg ttcgtcgggc gcatggcatg gcaagtgcgg cagcgcgacc
     2761 gcgagctcgc agcgatgcgc gagaacgcgc tgctcgatca gcaggtcatc gagctcggca
     2821 cgctcgcggc cggcacggcc catgagctga acacgccgct gtccacgctc gcgatactcg
     2881 cgagcgagct cgacgagacg acgacggacg cggcacagaa gcggcagttg cgcacgatgc
     2941 tcgaggagat ccgcgtcatc aacgagcgcc tcaacaggat cgcaggcggc gtcaatgccg
     3001 agcgcagcgc cggcgcgcgg cacgttgagc tcgacgcatt cctcgaggag ctgctcgcgc
     3061 agtgggcggc ggcgcaccgc gagatcgagc tcggcgtcgc cttcgccacg cagcgcaaca
     3121 acgtggccgt cgtcgtcgag gcgacaatcg agcaagcgat tcgcaacgtg ctcgacaacg
     3181 cggcgcacgc cacgctcgcg aacggcggcc gccgcatcga cgtctctatc agagtcgtcg
     3241 gcacgcggct cgagctcgcg gtgaaggatc aaggcgccgg gctcgatccc gccgtgcgcg
     3301 acgacatcgg cttgaaggca gtgtcgacga aagagcacgg cttggggata ggaatgttgc
     3361 tctcgcgggc cgcgctgcag cgtttcggcg gccgcctcga gctcaacgaa gcgccacgcg
     3421 gcggcgtcga ggcgcgcatc gagctgccgc tcgacgagct gctcgccgat gcccgctgac
     3481 gcgcggcgcg gcgtgctcgt cgtcgacgac gacgcgcggt tcgcggtcac gctcgcggcg
     3541 gcgctcgcgc gccgcggcta cgtggcgcac gtcgcgcacg acgcggcggc tgctctcgcg
     3601 acggcgcgcg cgaaggagcc ggacgcggcg gtcgtcgatc tgaagctcgg cgccgacgac
     3661 ggcctcgcgc tgattgagcc gctgcgcgcc gcgcatcctg acatgcgcat cgtcgtgctg
     3721 acgggttacg cgagcatcgc cacggccgtc aaagccatca agctcggagc cgacgactac
     3781 ctcgcgaaac ccgtgacggc cagcgccgtc gccgccgtcc tcgagcgcgg cacgcggaag
     3841 ccagcggaaa agcgctcggc cgagcccctc gagacgatgt cgccgcgccg gctcgagtgg
     3901 gagcacattc agcgcgtgct cgccgagcac gacggcaaca tctcggcgac ggcgcgtaca
     3961 ttgcgcatgc accggcgcac gctgcagcga aagctgctga aacggccgac ccagggctga
     4021 gccgcgcttt ggaacgcctg aacgcaaact ttccggcgct ccccgacccg acacgccggg
     4081 ctctggccac ggcgtgagaa gccggcgccg tcaggcgatt cgcggccggg cgtgccggat
     4141 ctgcagcaac gtgcgcccgg ccgggctcag cgtgtaagcc gtctcgccgt cgtatccgcc
     4201 gaccgtcgcg ttcagcaggt tgagcttgcg cagccgc
//
LOCUS       DASSNN010000808         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_720128, whole genome shotgun sequence.
ACCESSION   DASSNN010000808 DASSNN010000000
VERSION     DASSNN010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720128"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..534)
                     /locus_tag="VFL84_15175"
     CDS             complement(28..534)
                     /locus_tag="VFL84_15175"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET7610022.1"
                     /translation="MSEHTATPAALSAHVREEIDHWVAKFPEGRQRSAVIAALHAAQH
                     ENGGYLTPELMMAVAEYLKLPPIQVFEVATFYSMFETKPVGRHSISVCTNISCMLRGG
                     DDILAHVERRLGIKAGQSTPDGKFYLKVEEECLAACCGAPMMMIDHVYHENLTPKSVD
                     EILEAVKD"
     gene            complement(531..1784)
                     /locus_tag="VFL84_15180"
     CDS             complement(531..1784)
                     /locus_tag="VFL84_15180"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HET7610023.1"
                     /translation="MPEIQNFTLNFGPQHPAAHGVLRLVLELDGEVIQKADPHVGLLH
                     RATEKLAETKPFNQSIGYMDRLDYVSMMCNEHAYVLAIERLLGITPPIRAQYIRVMFD
                     EITRILNHLMWLGAHGLDIGAMAMFLYCFREREDLMDCYEAVSGTRMHATYYRPGGVR
                     LELPDSMPKYQPSPNRDAKELERINAARSGSLLDFIEDFTRRFPKNVDEYETLLTDNR
                     IWKQRTVNIGVVSPERAMQLGFSGPMLRGSGIAWDLRKKQPYEVYDKMDFDVPVGVNG
                     DCYDRYLVRVEEMRQANRIIKQCVDWLRANPGPINVDDHKIVPPRRIEMKDDMESLIH
                     HFKLFTEGYCVPEGESYAAIEGPKGELGVYLISDGANKPYRLKVRPPGFPHLASLNEM
                     VVGHMLADVVAVIGTQDIVFGEIDR"
     gene            complement(1777..>2181)
                     /locus_tag="VFL84_15185"
     CDS             complement(1777..>2181)
                     /locus_tag="VFL84_15185"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET7610024.1"
                     /translation="EGRFAVVYHLLSITHNHRLRLKVFCASDERPIVDSVYDVWNAAD
                     WFEREAFDLYGILFRGHPDLRRLLTDYGFIGHPFRKDFPLIGNVEMRYDPAKGRVVYE
                     PVSIVSRTLVPKVIRDDARYEPDLKVRDPGSA"
BASE COUNT          373 a          687 c          701 g          420 t
ORIGIN      
        1 tgattgaatt gtcgttcggc cacggcgcta gtccttcacg gcctcgagaa tctcgtcgac
       61 ggacttcggc gtcaggttct cgtggtaaac gtgatcgatc atcatcatcg gcgcgccgca
      121 gcacgccgcg aggcattctt cctcgacctt caagtagaac ttgccgtcgg gcgtgctctg
      181 ccccgccttg atgccgagcc gcctctcgac gtgcgcgagg atgtcgtcgc cgccgcgcag
      241 catgcacgag atgttcgtgc acaccgagat gctgtggcgg ccgaccggct tcgtctcgaa
      301 catcgagtag aacgtcgcga cctcgaatac ctggatcggc ggcagcttca agtattccgc
      361 gacggccatc atcagctccg gcgtgaggta gccgccgttc tcgtgctgcg cggcatgcag
      421 cgccgcgatc acggccgagc gctgccggcc ttccggaaac ttcgcgaccc agtggtcgat
      481 ttcctcgcgg acgtgcgccg atagcgcggc cggtgtcgcg gtgtgctcgc tcatcgatcg
      541 atctcgccga acacgatgtc ttgcgtgccg attacggcga cgacgtcggc cagcatgtgg
      601 ccgacgacca tctcgttcag cgacgcgaga tgcgggaacc cgggcggcct gaccttgagc
      661 cggtacggct tgttggcccc gtcggagatc aagtagacgc cgagctcgcc tttcggccct
      721 tcgattgccg cgtacgactc gccttcgggc acgcaatagc cttcggtgaa caatttgaag
      781 tggtggatca aggactccat gtcgtccttc atctcgatgc gccgcggcgg aacgatcttg
      841 tggtcgtcga cgttgatcgg accgggattc gcgcgcagcc aatcgacgca ctgcttgatg
      901 atgcggttcg cctgccgcat ttcctcgacg cgcacgagat agcggtcata acaatcgccg
      961 ttgacgccta cgggcacgtc gaagtccatc ttgtcgtaga cctcgtacgg ctgcttcttg
     1021 cgcagatccc acgcaatccc cgagccgcgc agcatcgggc ccgagaagcc gagctgcatc
     1081 gcgcgctccg gcgacacgac gccgatgttg acggtgcgct gcttccagat gcggttgtcc
     1141 gtcagcagag tctcgtactc gtcgacgttc ttcgggaacc gccgcgtgaa atcctcgatg
     1201 aaatcgagca gcgagccgct gcgcgccgcg ttgatgcgct cgagctcctt cgcgtcgcgg
     1261 ttcggcgagg gctggtactt cggcatgctg tccggcaact cgaggcgcac gccgcccggg
     1321 cgatagtacg tcgcgtgcat gcgcgtgccc gagacggcct cgtagcagtc catgaggtcc
     1381 tcgcgctcgc ggaagcagta caggaacatc gccatcgcgc cgatgtcgag gccgtgagcg
     1441 ccgagccaca tcaagtggtt caagatgcgc gtgatctcgt cgaacatgac gcggatgtac
     1501 tgcgcgcgta tcggcggcgt gatgccgagt aaacgttcga tcgcgagcac gtaagcgtgc
     1561 tcgttgcaca tcatcgagac gtagtcgagc cgatccatgt agccgatcga ctgattgaac
     1621 ggcttcgtct cggcgagctt ctcggtggcg cgatgcagca gcccgacgtg cgggtcggcc
     1681 ttctggatga cctcgccgtc gagctcgagc acgagcctca gcacgccgtg cgcggccggg
     1741 tgctgcgggc cgaaattcag tgtgaagttt tggatctcag gcactgccgg gatcccttac
     1801 tttgaggtcg ggctcgtagc gcgcgtcgtc gcgaatgacc ttcggcacga gcgtgcgcga
     1861 gacgatgctg acgggctcat agacgacgcg gcctttcgcc gggtcgtagc gcatctcgac
     1921 gttgccgatc agcgggaagt ccttgcggaa cgggtggccg atgaagccgt agtccgtgag
     1981 caagcggcgc aggtccgggt gcccgcggaa caagatgccg tacaggtcga acgcttcgcg
     2041 ctcgaaccaa tccgcggcgt tccagacgtc atagacggaa tcgacgatcg gccgctcgtc
     2101 gctcgcgcag aacaccttga gccgcaagcg gtgattgtgc gtgatcgaca gcaagtgata
     2161 gacgacggcg aagcggccct c
//
LOCUS       DASSNN010000809         5007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1735824, whole genome shotgun sequence.
ACCESSION   DASSNN010000809 DASSNN010000000
VERSION     DASSNN010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735824"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /gene="dctP"
                     /locus_tag="VFL84_15190"
     CDS             complement(<1..379)
                     /gene="dctP"
                     /locus_tag="VFL84_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016850027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET7610025.1"
                     /translation="MVTRRAVVQALAACATAGSARPAERQASLTAADVHPKDYPTVAA
                     VGWINEQLAAEFGGELTLRQYHSGQLGSEKDTLELARIGALAITRVHSSVLNNVVPAT
                     RILSLPYVFDSTEHMRRAFDGRFG"
     gene            complement(373..1224)
                     /locus_tag="VFL84_15195"
     CDS             complement(373..1224)
                     /locus_tag="VFL84_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HET7610026.1"
                     /translation="MLEKTGARAFGAEAELIAKRFVTARRTGEALLGFPGPLPPDLAA
                     AYACQDAAIELVPDTIAGWKVGLIGKEAAAIFEQDRLAGPIFSSSVRESTAGEPVGFP
                     VFDGGFAAVEAEFLLRLGRDAPEGQREWTRAQARELVADVHVGVETAGSPLAAINDLG
                     PTAIVADFGNNAGLIVGPRLRNWRTTAVEHWHCETFVDGTAVGRGHAGLPPGGPFESL
                     RFLLELCARRARPLKAGAWVSTGAITGVHQVVAGQTARVSFGDSGEVHCRAEPHRARV
                     AADAARW"
     gene            1339..2703
                     /locus_tag="VFL84_15200"
     CDS             1339..2703
                     /locus_tag="VFL84_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HET7610027.1"
                     /translation="MTAIVKTLAAGLAIACVALSAAAQGQRQTLQAAPLAFPGAVGWA
                     ATTPGGRGGRIVKVTTLANDGDGSLRAALALAEPRIIVFEVGGVIDLARQTLRITEPN
                     VTIAGQTAPSPGITLIQGGIDVLTHDVVLQHIRVRPGEAGQPKAADGGNWGEDAFSSQ
                     GGASDVIVDHCTFTWATDENLSASGPRFTGAGPDDWRRGTSHRITFSHNIIAEGLASS
                     THPKFEHSKGSLIHDNVTDILIYGNLYAHDVERSPLFKGGVHGTIVNNFIYDPGTRAV
                     HYNLQPLEWGDAPYQNGRMTAVGNVLRAGPSTHADLAFLMIGGAGDLDYHGRDNVAVD
                     RLGRPLPMFGRYTTSAARIIEHAEPLDWPAGLTAVPAVEVERWVLANVGARPWDRDAH
                     DTRLLADVTEGRGKIIDSEKEVGGYPRVPPTRRAFDPELWDLATMAPRSPAALDNSAK
                     APGT"
     gene            2730..3797
                     /locus_tag="VFL84_15205"
     CDS             2730..3797
                     /locus_tag="VFL84_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7610028.1"
                     /translation="MTKDKQARAPRTRHRARDKAKVTINDIARLANVSKKTVSRVIND
                     SPLVRDETRQKVKLIISEHGFAPDPQARALAFRRSFLIALIYDNPSPQYVVNMQRGIL
                     DTLEGTSFQLIVRPCDRSDPHFHASMRSFIEQQNLFGAILPPSVSEDQRLADILTAAG
                     CPYVRIASVVLDEPANMIKTHDSEGAAAAARHIATLGHTRIAHIRGPSTFRSSHERLR
                     GFEEGLAEFGLTLDPKLVLEGGYTFESGVACAGQLLERPPNARPTAVFAGNDEMAVGV
                     YQAARRAGFGVPEDLSIVGFDDTPMASRIWPPLTTVRLPIRDMGKAAAALLVHEHGDS
                     SSLVAFTPEIIVRQSTAPLRK"
     gene            3815..4657
                     /gene="kduI"
                     /locus_tag="VFL84_15210"
     CDS             3815..4657
                     /gene="kduI"
                     /locus_tag="VFL84_15210"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HET7610029.1"
                     /translation="MFTKTYQATHPDMMPDVRNEQLRERYLVAGLFAPDKIALNYSHN
                     ERFVIGGAAPVRGALELPAQAEPKAGVPFLERRELGLVNVGLGDGVVRADKEAVRLRP
                     RDGLYVPMGTARVAFESVDAAAPAKFYLVSTPAHARFELVKISIDAAVPLKRGALATS
                     NERTIYQYIVPQTCKSAQLLLGLTTLETGSVWNTMPPHLHDRRSEVYFYFGLGDNERV
                     FHFMGEPDKTRHIVVRNDEAVISPPWSIHMGSGTSSYSFIWAMGGENLDYTDMKVLDI
                     CQLQ"
     gene            4654..>5007
                     /locus_tag="VFL84_15215"
     CDS             4654..>5007
                     /locus_tag="VFL84_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003594832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7610030.1"
                     /translation="MTAGAAMFSLANRTALVTGANAGLGRAFAAALARAGADIVAVGR
                     KPPTETAADVAALGRRLEFVEADLDKARPADIVAKATGKFDGVDILVNNAGIIRRKDA
                     LELDEQDWDEVMNVNL"
BASE COUNT          817 a         1790 c         1678 g          722 t
ORIGIN      
        1 tgccgaacct gccgtcgaac gctcgccgca tgtgctcggt cgagtcgaac acgtacggca
       61 gcgacaggat ccgcgtggcc ggcacgacgt tgttcagcac ggagctgtgc acgcgcgtga
      121 tcgcgagcgc gccgatgcgc gcgagctcga gcgtgtcctt ctcgctgccg agctgccccg
      181 agtgatactg gcgcagcgtg agctctccgc cgaattcggc cgcgagctgc tcgttgatcc
      241 agccgacggc ggcgacggtc ggatagtcct tcggatgcac gtcggcggcc gtcagcgaag
      301 cctgacgctc cgccggccgc gcagagccgg ccgtcgcgca agccgcgagt gcctgcacga
      361 cggcgcgccg cgtcaccatc gcgcagcgtc cgcggcgacg cgcgctcgat gtggctcggc
      421 gcgacagtga acctcgccgg agtcgccgaa cgagacgcgc gccgtctggc ccgccacgac
      481 ctggtgcacg ccggtaatcg cgcccgtcga gacccaagcg cctgccttca gcgggcgcgc
      541 gcggcgagcg cagagctcga gcaggaagcg tagcgactcg aacgggccgc cgggcggcaa
      601 gccggcatgg ccgcggccga cggcggtgcc gtcgacgaac gtctcgcagt gccaatgctc
      661 gacggccgtc gtgcgccagt tgcgaagcct gggaccgacg atcagccctg cgttgttgcc
      721 gaagtcggcg acgatcgccg tggggccgag gtcgttgatc gccgcgagcg ggctgcccgc
      781 cgtctccacg ccgacgtgga cgtcggccac gagctcgcgc gcctgcgcgc gcgtccactc
      841 gcgctggcct tccggcgcgt cgcggccgag ccgcagcagg aattcggcct cgacggccgc
      901 gaagccgccg tcgaagaccg ggaaaccgac cggctcgccg gccgtcgact cgcgcacgct
      961 gctcgagaaa atcggtcccg cgaggcggtc ctgctcgaag atcgccgcgg cttccttgcc
     1021 gatcaaccct accttccagc cggcgatcgt gtcggggacg agctcgatcg cggcgtcctg
     1081 acatgcgtac gcagcggcga ggtcgggcgg cagcgggccg ggaaacccaa gcagcgcctc
     1141 gccggttcgg cgcgccgtga cgaagcgctt cgcaatcaac tccgcctccg cgccgaaggc
     1201 tcgagcgccg gtcttttcga gcacgaaggg gccctttcgt ccacgtccgt cgatgccggc
     1261 cagggtatgg gaaaccggtg tcattatcaa tatactggcc ttcctcggaa cgggcggaga
     1321 ccgatcgtgg cacacgccgt gacagctatc gtgaagacgc tggccgcggg cctcgccatc
     1381 gcctgcgtcg ctttatcggc ggccgctcaa ggtcaacgcc agaccctcca agcggcaccg
     1441 ctcgcctttc caggtgcggt cggctgggcg gccacgacgc cgggcgggcg cggcggccgc
     1501 atcgtcaagg tcacgacgct cgcgaacgac ggcgacggct cgctgcgcgc ggccctcgcc
     1561 ctcgccgagc cgcgcatcat cgtgttcgaa gtcggcggcg tcatcgatct tgcgcgccag
     1621 acgctgcgca tcacggagcc gaacgtcacg atcgcgggcc agacggcgcc ctcccccggc
     1681 atcacgctga ttcaaggcgg catcgacgtg ctcacccacg acgtcgtgct gcagcacatc
     1741 cgcgtccggc ccggcgaagc gggtcagccc aaagccgcgg acggcggcaa ctggggcgag
     1801 gacgcgttct cgagccaggg cggcgcgagc gacgtcatcg tcgatcactg caccttcacg
     1861 tgggcgaccg acgagaacct gtccgcgtcg gggccgcgct tcacgggcgc cgggccggac
     1921 gactggcggc gcggcacgtc gcaccgcatc acgttcagcc acaacatcat tgccgagggg
     1981 ctcgcgagct cgacgcaccc gaagttcgag cactcgaagg gctcgttgat ccacgacaac
     2041 gtcaccgaca tcctgatcta cggcaacctc tacgcgcacg acgtcgagcg cagcccattg
     2101 ttcaaaggcg gcgtccacgg cacgatcgtg aacaacttca tctacgatcc cggcactcgc
     2161 gccgtgcact acaacttgca accgctcgaa tggggcgacg cgccgtacca gaacggccgc
     2221 atgacggccg tcggcaacgt gctgcgcgcg gggccgtcga cgcacgccga tctcgcgttc
     2281 ctgatgatcg gcggggccgg cgacctcgac tatcacggcc gcgacaacgt ggccgtggac
     2341 cggctcggcc gcccgctgcc gatgttcggc cgctacacga cgagcgccgc gcgcatcatc
     2401 gagcacgccg agccgctcga ttggcccgcg gggcttacgg ccgtgccggc cgtcgaggtc
     2461 gaacgctggg tgctcgcgaa cgtcggcgcc cggccttggg accgcgacgc gcacgacact
     2521 cggctcttgg ccgacgtgac cgaaggccgc ggcaagatca tcgacagcga gaaagaggtg
     2581 ggcgggtatc cgcgcgtgcc gccgacccgc cgcgcgttcg accccgagct ctgggacctc
     2641 gcgaccatgg cgccgcggtc gcccgcggcc ctcgacaata gcgcgaaggc cccggggacc
     2701 tgagcgaaaa cgagaacgac gaggaccgag tgacgaagga caagcaggct cgcgccccgc
     2761 gcacgcggca tcgggcgcgc gacaaggcca aggtcacgat caacgacatc gcgcggctcg
     2821 cgaacgtttc gaagaagacc gtctcgcgcg tcatcaacga ttcgccgctc gttcgcgacg
     2881 agacgcgcca gaaggtcaag ctcatcatct ccgagcacgg cttcgcgccc gatccgcaag
     2941 cgcgcgcgct cgcgtttcgc cgctcgttcc tgatcgcgct gatctacgac aacccgagcc
     3001 cgcagtacgt cgtcaacatg cagcgcggca ttctcgacac gctcgagggc acgagctttc
     3061 agctgatcgt gcgcccgtgc gaccgcagcg atccgcactt tcacgcgagc atgcgctcgt
     3121 tcatcgagca gcagaatttg ttcggcgcca tcctgccgcc gtccgtgtcg gaggaccagc
     3181 ggctcgccga catcttgacc gcggcaggct gtccttacgt gcgcatcgcg tcggtcgtgc
     3241 tcgacgagcc ggccaacatg atcaagaccc acgacagcga gggagcggcc gcggccgcgc
     3301 gtcacatcgc gacgctcggc cacacgcgca tcgcgcacat ccggggcccg tcgacgttcc
     3361 gctcgtcgca cgagcggctg cgcggcttcg aggaagggct cgccgagttc ggcctcacgc
     3421 tcgatccgaa gctcgtgctc gaaggaggct atacgttcga atcgggcgtg gcctgcgcgg
     3481 gtcagctgct cgagcgcccg ccgaacgcgc ggccgaccgc cgtattcgcc ggcaacgacg
     3541 agatggcggt cggcgtctat caggccgcgc ggcgcgccgg gttcggcgtg cccgaggatc
     3601 tgtcgatcgt cggcttcgac gacacgccga tggcatcgcg aatctggccg ccgttgacga
     3661 cggtgcgcct gccgatccgc gacatgggca aggcggccgc ggcactgctc gttcatgagc
     3721 acggcgactc gtcgagcctc gtcgcgttca cgcccgagat catcgtccgc caatctacgg
     3781 cgccgctgcg gaagtaaccg gttcggagtc gcacgtgttc acgaagacct atcaagccac
     3841 gcacccggac atgatgcccg acgtccgcaa cgagcagctg cgcgagcgct atctcgtggc
     3901 gggattgttc gcgccggaca aaatcgcgct gaactactcg cacaacgaac gcttcgtgat
     3961 cggcggcgcg gcgcccgtcc gcggcgcgct cgagctcccc gcacaggccg agccgaaggc
     4021 gggcgtgccg tttctcgagc ggcgcgagct cgggctcgtc aacgtcggct tgggcgacgg
     4081 cgtcgttcgc gcggacaagg aagcggttcg gctgcgcccg cgtgacgggc tctacgtgcc
     4141 gatgggcacg gcgcgcgtcg cgttcgagtc cgtcgacgcc gccgcgcccg cgaagttcta
     4201 tctcgtctcg acgcccgcgc atgcgcgctt cgagctcgtg aagatctcga tcgacgcggc
     4261 ggtgccgctg aagcgcggcg cgctcgcgac gtcgaacgag cgcacgatct accaatacat
     4321 cgtgccgcag acgtgcaagt ccgcgcagct cttgctcggg ctcacgacgc tcgagaccgg
     4381 cagcgtctgg aacacgatgc cgccgcatct gcacgatcgc cgctccgagg tctatttcta
     4441 tttcgggctc ggcgacaacg agcgcgtgtt tcacttcatg ggcgagcccg acaagacgcg
     4501 gcacatcgtc gtccgcaacg acgaagccgt gatctcgccg ccgtggtcga tccacatggg
     4561 ctcgggcact tcgagctact cgttcatctg ggcgatgggc ggcgagaatc tcgactacac
     4621 cgacatgaaa gtgctcgaca tctgccagct ccaatgaccg ccggcgccgc gatgttcagc
     4681 ctcgcgaatc gcaccgcgct cgtgacgggc gcgaacgcgg ggctcggccg cgcgttcgca
     4741 gccgcgctcg cgcgcgccgg agccgacatc gtcgcggtcg gccgcaagcc gccgaccgag
     4801 acggccgcgg acgtcgcagc cttaggaagg cgcctcgagt tcgtcgaggc cgatctcgac
     4861 aaggcgcgcc ccgccgacat cgtcgcgaag gcgaccggga aattcgacgg cgtcgacatc
     4921 ctcgtcaaca acgccggcat catccgccgc aaagacgcgc tcgagctcga cgagcaggac
     4981 tgggatgagg tcatgaacgt caacttg
//
LOCUS       DASSNN010000810         5114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_721030, whole genome shotgun sequence.
ACCESSION   DASSNN010000810 DASSNN010000000
VERSION     DASSNN010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721030"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..4684
                     /gene="gltB"
                     /locus_tag="VFL84_15220"
     CDS             92..4684
                     /gene="gltB"
                     /locus_tag="VFL84_15220"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HET7610031.1"
                     /translation="MREPKSTGLPPAQGLYDPKFEHDSCGVGFVAHIKGKRSHKIIED
                     ADHLLCRMDHRGARGAEPNTGDGAGMLTALPHEFLAKAAKQAFGATLPAPGRFAAGIV
                     FLPQDAAERERCKSVVASICAEEGQVLVGWRAVPTNADAADLGAGAREAAPHIEQLFV
                     AAANGCEGDAFERKLYQLRKRASHRLRGDTSLREAKQFYVCSLSSKVLIYKGMLTPGQ
                     LLNFYPDLRDPDYTTHLAMVHSRFSTNTFPSWDRAQPNRFMSHNGEINTLRGNENWMR
                     ARQGVVKSELFGDSLKNLFPIAEPDCSDSGVFDNVLEFLLMTGRTLQESIMMMIPEAW
                     QKNELMPDNKRAFYEYHSCLMEPWDGPASIAFTDGKYIGAVLDRNGLRPSRYYLTHDD
                     RVIMASEVGVVPIDPANVKAKGRLEPGRMFLVDFERGRLVPDDELKNDFANRRPYGEW
                     LDANRIALRQVAAPPQEPVVDRASLVPRMQAFGYTIETMHFMLQPMVREHRDPVGSMG
                     NDSTLAVLSDKPRMLYDYFRQLFAQVTNPPIDSIREEVIMSLECYVGPELNLLETTPK
                     HAQRLRFRQPILSNEELDGIKNLSYRGWRTRTIDITYERKDAPGGLRGTIDRICADAE
                     RAIDDGYSMIVLSDRAVSRERLPVSALLACGAVHHHLVARAKRTRIGIVLETGEAREV
                     HHHCLLVGYGADGINPYLAYEALVQARNEGQLEPDIDIVGDYRQAVSKGMLKVMAKMG
                     ISTLQSYKGGQIFEALGLADEVIERCFHGTVSRLQGVNFAVLEQEALRRHTLAYPDDP
                     ADKLSSLPNPGEFHWRSGGERHAWDPQAIFSLQVAARRGDKNAYKQFAAHINEEARKT
                     GALRGLLDFATATKPLPLEQVEPASEIVKRFCTGAMSFGSLSTEAHETLAIAMNRIGG
                     KSNTGEGGEDPARFVPLPNGDSRRSAIKQVASGRFGVTIWYLANADELQIKMAQGAKP
                     GEGGELPGKKVDDNIARIRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANPSARV
                     SVKLVSEVGVGTIAAGVVKAHADHVLISGDNGGTGASPLTSIKHAGLPWELGLAETHQ
                     TLVMNDLRSRAVVQTDGGLKTGRDVVIAALLGAEEMGFSTAPLIAMGCIMMRKCHLNT
                     CPVGVATQDPVLRKKFAGQPEHVVNYLFMVAEEAREIMASLGVRRFVDLVGRTDLLQT
                     RAAINHWKADGLDLTPLLAPAVKPRPNTEVYCTKWQDHGLDLALDNKLIALARKSIQH
                     RKKTREELPISNINRTVGAMLSYEVVKKWGAEALPYDTVRFKFTGTAGQSFGAFLAKG
                     ITLELEGDSNDYVGKGLSGGRLIIYPPKNATFKAEENVLIGNVALYGATSGRAFFRGR
                     AAERFCVRNSGAKAVVEGVGDHGCEYMTGGRAVILGPTGRNFAAGMSGGIAYVWDPHK
                     DFAARCNTGTVDLDAVEEDEDIVELREMIELHREYTGSPVAAKILDDWPTSLRKFVKV
                     MPTDYKRVLAERKRHDEEMEQKLRDDETGTFVAQAR"
     gene            4716..>5114
                     /gene="gltD"
                     /locus_tag="VFL84_15225"
     CDS             4716..>5114
                     /gene="gltD"
                     /locus_tag="VFL84_15225"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661229.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HET7610032.1"
                     /translation="MGKPTGFMEYPKKSVPYRDARERIQDFREIFTQATEDHLHNQSG
                     RCMDCGVPFCQSDNGCPIDNLIPEWNDMVYQGRWREALDRLHKTNNFPEFTGRTCPAP
                     CEGACVLGITDPAVTIKNIENAIIDRGFAEG"
BASE COUNT          962 a         1710 c         1671 g          771 t
ORIGIN      
        1 gctacgacca cgaaactgca tcgacgcggc gcgcccaaac gggctcgcgg ccggtgcgtc
       61 gtgaacgctc ttgcggcatg aggacggcaa gatgcgtgaa ccaaagagca ccggcctccc
      121 gccggcacaa gggttgtacg accccaagtt cgagcacgac agctgcggcg tcggcttcgt
      181 tgcgcacatc aaaggcaagc gcagccacaa gatcatagag gacgcggatc acctcctctg
      241 ccgcatggac catcgcggcg cacgcggtgc cgagcccaac acgggcgacg gcgccggcat
      301 gctgacggcg ctgccgcacg agttcctcgc gaaggccgcg aagcaggcgt tcggcgccac
      361 gttgccggcg ccgggcagat tcgcggccgg catcgtgttc ctgccgcagg acgcggccga
      421 gcgcgagcgc tgcaagtccg tcgtcgcgtc gatctgcgcc gaagaaggcc aggttctcgt
      481 cggctggcgc gcggttccga cgaacgccga cgcggcggac ttgggtgcgg gcgcgcgcga
      541 ggccgccccg cacatcgagc agctcttcgt cgccgcggcg aacggctgcg agggcgacgc
      601 gttcgagcgc aagctctacc agctccgcaa gcgcgcgagc caccggttgc gcggcgacac
      661 gtcgctgcgc gaggcgaagc agttctacgt ctgcagcctc tcgagcaagg tgctcatcta
      721 caagggcatg ctcacgccgg ggcagttgct gaatttctat ccggatctac gcgatcccga
      781 ttacacgacg catctcgcga tggtccattc gcggttctcg acgaacacgt tcccgagctg
      841 ggaccgtgcg cagccgaatc gcttcatgtc gcacaacggc gagatcaaca cgctgcgcgg
      901 caacgagaac tggatgcgcg cgcgccaggg cgtcgtaaag agcgagctct tcggcgactc
      961 gctgaagaac ctgttcccga tcgccgagcc cgactgctcc gactcgggcg tcttcgacaa
     1021 cgtcctcgag ttcctgctga tgacgggccg cacgctccag gaatccatca tgatgatgat
     1081 tccggaggca tggcagaaga acgagctgat gcccgacaac aagcgggcgt tctacgagta
     1141 ccactcgtgc ctcatggagc cgtgggacgg cccggcctcg atcgcgttca cggacggcaa
     1201 gtacatcggc gccgtgctcg atcgcaacgg cttgcggccg agccgctatt acttgacgca
     1261 cgacgaccgc gtcatcatgg cgagcgaggt cggcgtcgtg ccgatcgatc cggccaacgt
     1321 caaggccaag ggccggctcg agccgggccg catgttcctc gtcgacttcg agcgcggccg
     1381 gctcgtgccc gatgacgagc tcaagaacga cttcgcgaat cgccgcccgt acggcgagtg
     1441 gctcgacgcg aatcgcattg cgttgcgtca ggtcgcggcg ccgccgcagg agcctgtggt
     1501 cgaccgagcg tcgctcgtgc cgcgcatgca ggcgttcggc tacacgatcg agacgatgca
     1561 cttcatgctg cagccgatgg tgcgcgagca tcgcgacccg gtcggctcga tgggcaacga
     1621 ctcgacgctc gcggtgctct ccgacaagcc gcgcatgctc tacgactatt tccgccagct
     1681 cttcgcgcag gtgacgaacc cgccgatcga ctcgatccgc gaggaagtca tcatgtcgct
     1741 cgagtgttac gtgggccccg agctgaactt gctcgagacc acgccgaagc acgcgcagcg
     1801 cctgcggttc cgccagccga tcttgagcaa cgaagagctc gacggcatca agaacttgag
     1861 ctaccgcggc tggcgcactc gtacgatcga catcacgtac gagcgcaagg acgcgccggg
     1921 cgggctgcgc ggcacgatcg accgcatttg cgccgatgcg gagcgcgcga tcgacgacgg
     1981 ctactcgatg atcgtgctgt cggaccgcgc ggtgagccgc gaacggctgc ccgtgagcgc
     2041 gctgctcgcc tgcggcgcag tgcatcatca cctcgtcgcg cgcgcgaagc gcacgcgtat
     2101 cggcatcgtc ctcgagaccg gcgaggcgcg cgaggtccat caccattgcc tgctcgtcgg
     2161 ctacggcgcc gacggcatca atccgtacct cgcgtacgaa gcgctcgtgc aggcgcgcaa
     2221 cgaaggccag ctcgagcccg acatcgacat cgtcggcgat taccggcaag ccgtgagcaa
     2281 aggcatgctc aaggtcatgg cgaagatggg catctcgacg ctgcagtcct acaagggcgg
     2341 gcagattttc gaggcgctcg ggctcgccga cgaggtcatc gagcgctgtt tccacggcac
     2401 ggtcagccgc ctgcagggcg tgaacttcgc cgtgctcgag caggaggccc tgcggcgcca
     2461 cacgctcgcg taccccgacg acccggccga caagctgtcg tcgttgccga accccggcga
     2521 gttccactgg cgcagcggcg gcgagcgcca cgcgtgggat ccgcaagcga tcttctcgct
     2581 ccaggtggcc gcacgccgcg gcgacaagaa cgcgtacaag cagttcgccg cgcacatcaa
     2641 cgaagaagcg cgcaagacgg gcgcgctgcg cggcctgctc gacttcgcta cggccacgaa
     2701 gccgttgccg ctcgagcagg tcgagccggc cagcgaaatc gtcaagcgtt tctgcacggg
     2761 cgcgatgagc ttcggctcgc tgtcgaccga ggcgcacgag acgctcgcga tcgcgatgaa
     2821 caggatcggc ggcaagagca acacgggcga aggcggcgag gatcccgcgc gcttcgtgcc
     2881 gctgccgaac ggcgactcgc gccggtcggc gatcaagcaa gtcgcctccg gccgcttcgg
     2941 cgtcacgatc tggtatctcg cgaacgccga cgaattgcag atcaagatgg cgcagggcgc
     3001 gaagcccggc gagggcggcg agctgcccgg caagaaggtc gacgacaaca tcgcccggat
     3061 tcgccactcg actcccggcg tcggcttgat cagcccgccg ccgcatcacg acatctattc
     3121 gatcgaggat ctcgcgcagc tcatacacga cttgaagaac gcgaacccgt cggcacgcgt
     3181 cagcgtcaag ctcgtgtcgg aggtcggcgt cggcacgatc gcggccggcg tcgtgaaagc
     3241 gcatgccgat cacgtgctga tctcgggcga caacggcggc acgggcgcct caccactcac
     3301 gagcatcaag cacgcgggct tgccgtggga gctcgggctc gccgagacgc atcagacgct
     3361 cgtcatgaac gatctgcgca gccgcgccgt ggtccagacc gacggcggct tgaagaccgg
     3421 ccgcgacgtc gtcatcgcag cgctgctcgg cgccgaggag atgggcttct cgacggcgcc
     3481 gttgatcgcc atggggtgca tcatgatgcg caagtgccac ttgaatactt gcccggtcgg
     3541 cgtcgcgacg caggaccccg tgctgcgcaa gaaattcgcg ggccaacccg agcacgtcgt
     3601 gaactacttg ttcatggtgg ccgaggaagc gcgcgagatc atggcgagcc taggtgttcg
     3661 ccgcttcgtc gatcttgtag gccgcaccga cctgctgcag acgcgcgccg ctatcaacca
     3721 ctggaaggcc gacggcctcg acctgactcc gttgctagcg ccggccgtca agccgcggcc
     3781 gaacacagag gtctattgca cgaagtggca agaccacggg ctcgacctcg cgctcgataa
     3841 caagctgatc gcgctcgccc gcaagtcgat ccagcaccgc aagaagacgc gcgaggagct
     3901 gccgatctcg aacatcaatc gcacggtcgg cgcaatgctg agctacgagg tcgtcaagaa
     3961 gtggggcgcc gaggcgctgc cttacgacac cgtgcgcttc aagtttacgg gcaccgccgg
     4021 ccagagcttc ggcgcattcc tcgcgaaggg catcacgctc gagctcgaag gcgacagcaa
     4081 cgactacgtc ggcaagggcc tctcgggcgg ccgcttgatc atctacccgc cgaagaacgc
     4141 gacgttcaag gccgaggaga acgtgctgat cggcaacgtc gcgctgtacg gcgccacgag
     4201 cgggcgcgcg ttcttccgcg gccgcgcggc cgagcgtttc tgcgtgcgca acagcggcgc
     4261 caaagccgtc gtcgaagggg tcggcgatca cggctgcgaa tacatgacgg gcggccgcgc
     4321 ggtgattctg ggtccgacgg gccgcaactt cgcggccggc atgtcgggcg gtatcgctta
     4381 cgtctgggac ccgcacaagg acttcgctgc tcgctgcaat acgggcaccg tcgatctcga
     4441 cgccgtcgag gaagacgagg acatcgtcga gctacgcgag atgatcgagc ttcatcgcga
     4501 gtacaccggc tcgccggtcg ccgcgaagat tctcgacgac tggccgactt cgctccgcaa
     4561 gttcgtcaaa gtcatgccga cggactacaa gcgcgtgctc gccgagcgca agcgccacga
     4621 cgaagagatg gagcagaagc tccgtgacga cgagacggga acgttcgtcg ctcaagctcg
     4681 ctaactaaaa cgacaacgga ccttagtcct cgcttatggg caagcccacc ggcttcatgg
     4741 agtatccgaa gaagagcgtg ccgtatcgcg acgctcgcga gcgcattcaa gacttccgcg
     4801 agatcttcac gcaggcgacg gaagaccatt tgcacaacca gagcgggcgc tgcatggact
     4861 gcggcgtgcc gttctgccaa tcggacaacg gctgcccgat cgacaacctg attcccgagt
     4921 ggaacgacat ggtctaccag ggccggtggc gcgaagcgct cgaccggctg cacaagacca
     4981 acaacttccc cgagttcacg ggccgcacat gcccggcccc ctgcgagggc gcctgcgtgc
     5041 tcggtatcac ggacccggcc gtcacgatca agaacatcga gaacgcgatc atcgaccgcg
     5101 gcttcgcgga aggc
//
LOCUS       DASSNN010000811         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1356897, whole genome shotgun sequence.
ACCESSION   DASSNN010000811 DASSNN010000000
VERSION     DASSNN010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356897"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFL84_15230"
     CDS             complement(<1..962)
                     /locus_tag="VFL84_15230"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HET7610033.1"
                     /translation="MATTTKLSCLSVLAMLVVAAPLRAQPPASSSARQMTDAARALLA
                     VLDGAQLDVVLQALDDEERSQWSKVPYSSHHRPGLRLGRLDREQMLRVHDLLRASLSS
                     QGYQKAAGVIRLDDINRAQQVARLAPNATAFQKAQAEGFGSGNYFVLFFGDPRRDARW
                     GWLLQGHHLALNFTVADGKTAFLPMFVGATPLAVAENVETGWSALAQEVTRGVELVTA
                     LTESQRKAAISSAAVPEDVLNGVGNKDRFTPPEGLRAADMTPEQRRLLRALVEEYVRN
                     ADFDAADEQLAAIDAAGWDKVAFTWRGAIGDLAEPFYYRVQGPR"
     gene            complement(1016..>2006)
                     /locus_tag="VFL84_15235"
     CDS             complement(1016..>2006)
                     /locus_tag="VFL84_15235"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7610034.1"
                     /translation="IAREHRELRFAFSRPGFVTFRLPDASGAEFALRSVFARTWGHSL
                     GKVGGADDVALARDAWRLVAERLPDEPVRHVHVWQRDQPLPGDEGFDAAAGALANEIG
                     ALLVGQAAESAAPAAVNEVAAAGELVVDCVLVARDEWWLGWHRAQAPETRWPGGVPPI
                     ALPPRLISRAYLKIVEALEWSELPLAAGDRCVEIGSSPGGSCLALLERGLNVTGIDPA
                     EMDAEVLAHPNFTHVRARAKDVKRSVFRDCRWLVMDANVAPSYTFDTLDGVLTKGGAR
                     PAGLVLTLKLTDPKLTEKLPALADRLRAYGYGRVRMRQLAFNRQEVCAVADRP"
BASE COUNT          294 a          702 c          696 g          314 t
ORIGIN      
        1 atgcgcgggc cttgcacgcg gtagtagaac ggctcggcga ggtcgccgat ggcgccgcgc
       61 cacgtgaatg cgaccttgtc ccatccggcc gcgtcgatcg ccgcgagctg ctcgtcggcc
      121 gcgtcgaagt cggcgttgcg gacgtactcc tcgacgagcg cgcgcagcaa gcgccgttgc
      181 tcgggcgtca tgtcggccgc gcgcaagcct tcgggcggcg tgaagcggtc cttgttgcct
      241 acgccgttca agacgtcctc gggcactgcg gctgaggaga tcgccgcttt ccgttgactc
      301 tcggtcagcg ccgtgacgag ttcaacgcct cttgtcactt cttgtgccaa cgcggaccag
      361 cccgtctcca cgttctcggc gacggcgagc ggcgtcgcgc cgacgaacat cggcagaaac
      421 gcggtcttgc cgtcggcgac ggtgaagttg agcgcgagat ggtggccttg cagcagccat
      481 ccccaccgcg catctcttcg cgggtcgccg aagaagagaa cgaagtaatt gccggagccg
      541 aagccctcgg cttgcgcctt ctgaaacgcc gtggcgttcg gcgcaaggcg cgcgacttgc
      601 tgcgcgcgat tgatgtcgtc gaggcgaatg acgccggcgg ctttctggta gccctggctc
      661 gacagcgacg cgcgcaacaa gtcgtgcacg cgcagcatct gctcgcggtc gagccttcct
      721 aagcgcaggc cgggccgatg gtgcgacgag tacggcactt tcgaccattg cgagcgctct
      781 tcgtcgtcga gcgcttgcag cacaacgtcg agctgcgctc cgtcgagcac ggcgagcaac
      841 gcccgcgcgg cgtccgtcat ctgccgcgcg ctcgaagatg cgggcggttg cgcccgaagc
      901 ggcgccgcca cgacgagcat cgcaagcacg ctcaggcacg agagctttgt tgtcgtcgcc
      961 atcgcttgcg ctccgctcga atcagctcgc cgccatctta ccgccggccc cgtcgtcacg
     1021 ggcgatcggc gacggcgcag acttcttgcc ggttgaatgc gagctggcgc atgcgcacgc
     1081 gaccgtaacc gtaggcgcgc agccggtcgg cgagcgcggg aagcttttcc gtgagcttcg
     1141 gatccgtgag cttcagcgtc agcacgagac ccgcgggccg cgctccgccc tttgtcagca
     1201 cgccgtcgag cgtgtcgaac gtgtagctcg gcgcgacgtt cgcgtccatg acgagccagc
     1261 ggcagtcgcg gaacacgctg cgcttgacgt ccttggctcg cgcccggacg tgcgtgaagt
     1321 tggggtgggc gagcacctcg gcgtccatct cggcggggtc gatgcccgtc acgttgagac
     1381 cgcgctcgag cagcgcgaga cacgacccgc ccggcgagct gccgatctcg acgcagcggt
     1441 cgccggccgc gagcggcagc tccgaccatt cgagcgcttc gacgatcttc aagtacgcgc
     1501 gcgagatcag gcgcggcggc agcgcgatcg gcggcacgcc gcccggccag cgcgtctcgg
     1561 gcgcttgcgc gcgatgccag ccgagccacc actcgtcgcg cgcgacgagc acgcagtcga
     1621 cgacgagctc gccggctgcg gccacttcgt tgacggccgc tggcgcggcg ctttccgctg
     1681 cttgcccgac gagcagcgcg ccgatctcgt tcgcgagcgc gccggcggcg gcgtcgaagc
     1741 cttcgtcgcc gggcagcggc tggtcgcgct gccagacgtg cacgtgccga acgggttcgt
     1801 ccggcagtcg ctccgcgacg agtcgccacg cgtcgcgtgc gagcgcgacg tcgtccgcgc
     1861 cgccgacttt gccgagcgaa tggccccagg tgcgcgcgaa cacgctgcgc agagcgaatt
     1921 cggcgccgct cgcgtccggg agccgaaacg tcacgaagcc cgggcgcgag aacgcgaagc
     1981 gaagctcgcg atgctcgcgc gcaatc
//
LOCUS       DASSNN010000812         6945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1738008, whole genome shotgun sequence.
ACCESSION   DASSNN010000812 DASSNN010000000
VERSION     DASSNN010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738008"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2164)
                     /locus_tag="VFL84_15240"
     CDS             complement(<1..2164)
                     /locus_tag="VFL84_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610035.1"
                     /translation="MCVGLGCAYAPLAAAQYQALHFDETALPPEQQQALNIVSYGSIF
                     ANIAVHNASIPIGFSPTPLISASAEFAGIAADNALKFTKAALNAPPNVTVSPNVAGNC
                     SYRFELPQATAEFQNFLGLWPKLGDDALLRSRDTPISFGVLGVPQILHAHTDVALSVF
                     GPTAGIANDGDGTAVDEEAPQTVLVPAGAHAIEWRADTLWYPVWDAVVPTALAAVMAA
                     MEFKFADNVAALTAQKSDALELASIKSNPGKLTAQLDHIDNALHLYERLDWRLFGADV
                     FFTVSEPVTSELIVERFFEPTKTVSRSRLQTLTVYDVLPPTITTSDAAPTKEATDIGG
                     ARTARYLPDLSSLITASDACGRPVTVTNDAPDFLPLGSTLVTWTVRDPGPSAPGVDHD
                     GDGVVDTQAFNSRTVTQLVTIKDTQAPIMVPPPGVVIESNASVDLANQALGQPLVVDL
                     ADLHPTVTRSSDSVDGIVPPDTRTVVTWSAADDGTPPNVTTAQQLVTVKTPGTNTAPT
                     ASPASADTLTSKAVPIVLTGFDADVLPLTAGPAGAGIRDPLQFKIESLPQHGELVAPL
                     YPFFIDDYRTDKVGGLIAYIESQPDAATLRQSYETAVATRNLENWLNVEFCDASPNQP
                     APVDFVFEPRYVQVTDAGEQYFFDNYLVCDPNPDEDWALRPRISRWSAAGEFLGHVRI
                     DDNGGGGIRPEEAAFRFSDDGFLYFVAKNTGGEPVVSVH"
     gene            complement(2408..4009)
                     /locus_tag="VFL84_15245"
     CDS             complement(2408..4009)
                     /locus_tag="VFL84_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610036.1"
                     /translation="MAESRNVTAAAAVPAAGAPSFTFSTRLSARGAFLVNAAFVAGML
                     VLSQLPLLDARPVVRASIVGAALVLLACSALLFGVLRRGQTVAFEVALRPQHYLQAFL
                     QGSLILYWGFYWREVYHAAPLIVAQLLFAYGFDSLLSWAHRRTFALGFGPFPIIFSLT
                     LFFWFKDEWFYWQFVMVAIGFLAKEFLRWQRDGRNTHIFNPSSFPLAVVSIALLMADS
                     TDMTWGFFVAQTEFYPPHMYLAIFLVGLPGQFLFGVAPMTFAAVTTTFVFSQIYYAAT
                     GSYYFVDAHIPIAVFIGMTLLFTDPATSPRTFVGRIAYGVLYGLTTVWLYDLLLDANK
                     PGFYDKLLQVPLLNLSVLLLDRLAAWPGLKAFDPSVWARDWAPRGRHLAYMSVYATAF
                     VAMSATGYLGDRHPGQWTPFWEQACAAEKRDACLNLYLLHDGYCTDGSAWSCNEIGIL
                     LAERYANRQNAAAAFRRACTQQFRAACDNAAALADGRPLRHDAPTAADYRFILRGSKG
                     PIDDVEPAKLYARACALGWPGTCES"
     gene            complement(4252..4476)
                     /locus_tag="VFL84_15250"
     CDS             complement(4252..4476)
                     /locus_tag="VFL84_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610037.1"
                     /translation="MATKELRELRHKYKAAYTTYMQCVQALSDASQAGVLPAAEVLKL
                     ENKALNELTELRQALLDALHAHTAKASGSS"
     gene            4883..5566
                     /locus_tag="VFL84_15255"
     CDS             4883..5566
                     /locus_tag="VFL84_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7610038.1"
                     /translation="MTNPQPTAGLAADAPDSELAARVAAGERAAFEALMRRHNRALFR
                     TARAILRDDAEAEDALQEAYLQAYRAIGGFRAEAKLGTWLARIVANEALMRVRKRARR
                     AEIVPLQAGVSETEINEIPDENMNETPERSAQRTEMRRLLEAQIDSLPDDYRVVFVLR
                     AVQEMSVEETSEALGIPPATVRTRLFRARSLLREGLAAKIDIACEEAFSFAGKRCDRI
                     VAHVLERMK"
     gene            5563..6135
                     /gene="folE"
                     /locus_tag="VFL84_15260"
     CDS             5563..6135
                     /gene="folE"
                     /locus_tag="VFL84_15260"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET7610039.1"
                     /translation="MTAVLKSVQLKDAADPVADHVRAIIEHLGEDTTRGGLAATPARY
                     AKALRYLAGGYSGDLESCIGSGLFPAEGESPVLMRDVRFYSLCEHHLLPFHGRIHVGY
                     IPSRRIIGLSKIPRIVDMFARRFQVQERLTEQVADALVQALEPKGVVVVSEAQHLCMA
                     MRGVQEHGATTTVRATRGVCEPLLAMLQRG"
     gene            6140..6325
                     /locus_tag="VFL84_15265"
     CDS             6140..6325
                     /locus_tag="VFL84_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HET7610040.1"
                     /translation="MRYRKFVCNVCGHVYDEALGDAAAGLAAGTRWEDIPEDWSCPEC
                     GATKADFTLLAAEAAVS"
     gene            6627..>6945
                     /locus_tag="VFL84_15270"
     CDS             6627..>6945
                     /locus_tag="VFL84_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610041.1"
                     /translation="MHFSWLWVFFGMFFWMLMMRPRRLWACQGRGFTDERRGFDDETR
                     GPLDRPSRRPRRADLESELARRDALIAKLEERVRVLERIATDDSARLRAEIDALRDPP
                     ASSG"
BASE COUNT         1200 a         2260 c         2377 g         1108 t
ORIGIN      
        1 ggtggacgga cacgaccggt tccccgccgg tattcttcgc gacgaaatac aaaaagccgt
       61 cgtcgctgaa gcgaaaggcc gcttcttcgg gccgtatgcc gccgccgccg ttgtcgtcga
      121 tgcgcacatg accgagaaac tcgcctgccg cgctccagcg cgagatgcgc gggcgcagcg
      181 cccagtcctc gtccgggttg gggtcgcaga ccaaatagtt atcgaagaaa tactgctcgc
      241 ctgcgtcggt aacttgcaca tagcgcggct cgaacacgaa gtcgacgggc gcaggttggt
      301 tcggactcgc gtcgcagaat tccacgttga gccagttctc taagttgcgc gtcgcgaccg
      361 cggtctcgta gctctggcgc agcgtcgccg cgtcgggctg tgactcgatg tatgcgatga
      421 gcccgccgac cttgtcagtg cggtaatcgt cgatgaagaa cggataaagc ggcgcgacga
      481 gctcgccatg ctggggcaac gattcgatct tgaactgcag cgggtcgcga atccccgcgc
      541 cagccggacc cgcggtcaaa ggcagcacgt cggcgtcgaa gccggtcagc acgatcggaa
      601 ccgccttcga cgtcagcgta tcggcggaag ccggcgacgc agtcggcgcc gtgttcgtgc
      661 ccggcgtctt gacggtcacg agctgctgcg ccgtcgtcac gttcggcggc gtgccgtcgt
      721 cggcggcgct ccaagtcacg acggtgcgcg tatcgggcgg cacgatcccg tcgacgctgt
      781 cgctcgaccg cgtgaccgtg gggtgcagat cggccagatc gacgacgagc ggctgtccga
      841 gcgcctgatt cgcgaggtcg accgatgcat tgctctcgat cacgactccg ggcggcggca
      901 ccatgatcgg cgcctgcgtg tcctttatcg tcacgagctg cgtcacggtg cgcgagttga
      961 acgcctgcgt gtcgacgacg ccgtcgccgt cgtgatcgac gcccggcgcc gatggaccgg
     1021 gatcgcgaac ggtccatgtc acgagcgtgg agccaagcgg tagaaaatcg ggcgcgtcgt
     1081 tcgtgaccgt cacggggcgg ccgcatgcgt cgctcgccgt gatcaggctg cttaggtcgg
     1141 gcagatagcg cgccgtgcga gcgccgccga tgtcggtcgc ttccttcgtc ggggcggcat
     1201 cgctcgtcgt tatcgtcggc ggcaggacgt catagaccgt gagcgtctgg agccgcgagc
     1261 gcgacactgt cttcgtgggc tcgaagaacc gctcgacgat gagctcgctc gtgacgggct
     1321 cggagaccgt gaagaacaca tcggcgccga agagccgcca atcgagccgc tcgtacaggt
     1381 gcagggcgtt gtcgatgtgg tcgagctgcg ccgtcagctt gccgggattg ctcttgatcg
     1441 acgcgagctc gagcgcgtcg cttttctgcg ctgtcaacgc cgcgacgttg tcggcgaact
     1501 tgaactccat ggcagccatg acagccgcga gcgccgtcgg caccacggcg tcccagacgg
     1561 gataccacag cgtatcggcg cgccattcga tggcgtgcgc gcctgccggc accagtacgg
     1621 tctgcggcgc ttcctcgtcg acggcagtgc cgtcgccgtc gttggcgatg cccgcggtcg
     1681 gaccgaacac gctgagcgcg acgtccgtat gcgcgtgcag aatctgcggc acgccgagga
     1741 cgccgaagct gatcggagtg tcgcgcgagc ggagcaacgc atcgtcgccg agcttgggcc
     1801 acaagccgag gaagttctgg aactcggccg tcgcttgcgg aagctcgaag cgatagctgc
     1861 aattcccggc gacgttcggc gacacggtca cattcggggg cgcgttgagc gccgccttcg
     1921 tgaacttcag cgcgttgtcg gcggcgatac ccgcgaactc cgcgctggcc gaaatgagcg
     1981 gtgtcggcga gaaaccgatt ggaatgctcg cgttgtggac cgcgatgttg gcgaagatgc
     2041 tgccgtacga aacgatgttg agcgcctgct gttgctcggg cggcagcgcg gtttcgtcga
     2101 aatgcagtgc ctgatattgc gcagccgcga gcggcgcgta ggcgcagccc aaccccacgc
     2161 acatggccct cacggcgcct gcaacgatgc ccgtcggaag acgggtctgc actggcttcg
     2221 acattcagca gcgccgcttt cgagcgcggc ccgcggcggg cgacgacatc tcgaggaatc
     2281 ccgcatcggg atgcgccatc cagggcgcag ctcggcgcta ttcgttgtta ttagttgccg
     2341 gagagtctac gacggtgcag cacgcagggt ccagaagact ttcgcgttat gtcccttcgc
     2401 ttggccgtca ggactcgcag gtgccgggcc agcccaacgc gcaagcgcgc gcgtagagct
     2461 tcgccggctc gacgtcgtcg atcggacctt tgctgccgcg caggatgaag cggtagtccg
     2521 ccgcggtcgg cgcgtcgtga cgcagcgggc gcccgtccgc gagcgcggcg gcattgtcgc
     2581 aggccgcccg gaattgctgc gtgcacgcgc gccggaacgc cgccgcggcg ttttgacgat
     2641 tggcgtagcg ctccgcgagc aagatgccga tctcgttgca cgaccaagcg gacccgtccg
     2701 tgcaatagcc gtcgtgcagg aggtacaggt tcaagcaagc gtcgcgcttc tcggccgcgc
     2761 acgcctgctc ccagaacggc gtccattgcc ccggatgccg gtcgccgaga taccccgtcg
     2821 cgctcatcgc gacgaacgcg gtcgcgtaga cgctcatgta ggcgagatgg cgcccgcgcg
     2881 gcgcccaatc gcgggcccac acggaaggat cgaacgcttt gagcccgggc cacgccgcca
     2941 atcgatcgag cagcaggacc gagaggttca gcagcggtac ttgcagcagc ttgtcgtaga
     3001 agccgggctt gtttgcgtcg agcagcaagt cgtacaacca aacggtcgtg agcccgtaaa
     3061 gcacgccgta ggcgatgcgt cccacgaacg tgcgcggcga cgtcgccggg tccgtgaaca
     3121 gcagcgtcat gccgatgaac acggcgatcg ggatatgcgc gtcgacgaag taatagctgc
     3181 ccgtcgccgc gtagtagatc tggctgaaga cgaacgtcgt cgtgacggca gcgaacgtca
     3241 tcggcgcgac gccgaacagg aattgcccgg gcagcccgac gaggaaaatt gcgaggtaca
     3301 tgtggggcgg atagaactcg gtctgcgcga cgaaaaagcc ccacgtcata tcggtgctat
     3361 cggccatcag cagcgcgatc gacactacgg ccagagggaa cgacgacgga ttgaagatgt
     3421 gcgtgttccg tccgtcgcgc tgccagcgca agaactcctt cgccaggaag ccgatcgcga
     3481 ccatcacgaa ctgccagtag aaccactcgt ccttgaacca gaagaacagc gtgaggctga
     3541 agatgatcgg gaacgggccg aagcccaaag cgaacgtgcg ccgatgcgcc cacgacaaga
     3601 gactgtcgaa gccgtacgcg aacagcagct gcgcgacgat caacggcgcc gcgtggtaca
     3661 cctcgcgcca gtagaagccc cagtagagga tcagcgagcc ctgtaggaag gcctgcaagt
     3721 aatgctgcgg ccgcagcgcg acttcgaacg cgacggtttg cccgcgccgc aagacgccga
     3781 acagcagcgc gctgcacgcg agcagcacga gcgcggcgcc gacgatcgag gcgcgcacga
     3841 cgggccgcgc gtcgagcagc ggaagctggc tcagcacgag catgccggcg acgaacgcgg
     3901 cgttgacgag gaaagcgccg cgcgccgaga gacgcgtgct gaaagtgaaa gacggcgcgc
     3961 ctgccgcggg aaccgcggct gccgccgtca cgttgcgcga ctccgccatc ggacgcaagc
     4021 cctcaagcga gcaccgtggc gaccacgagc agcgacacgc cgacgtaaaa cggactgcgg
     4081 gcggcgcgtg ctcgcggcgg cacgcgttcg acgaccacgg atgccatgct tcccccgaat
     4141 tgccttaggc gcagcgcggc gcgccggtca ttcttctcgc catcgtagcg agttcgacgc
     4201 gcttcggcac gggcgggctg caggatgcgc gcagtgccgc ctgcaggcgg ctcagctcga
     4261 tccagaggct ttcgcggtgt gcgcgtgcag cgcgtcgagc agcgcctgcc gcagctcggt
     4321 cagctcgttg agcgccttgt tctcgagctt cagcacttcc gcggccggca gcacgccggc
     4381 ctggctcgcg tcggataatg cctgtacgca ttgcatatag gtcgtatagg cggccttgta
     4441 cttatgcctc agctctcgaa gttccttcgt cgccatggtt tattctttat cgtccgcgcg
     4501 gcaggaccgg gcgccaccat atcgtcttgt gcctaagcgc tcaagcaaga agaaatctag
     4561 cgacgctaac caaaccgcag cgttctttgc ggctctggcc agtctcgccg cggattcacc
     4621 cagtgtcgcg gccaaggatc ccgccgcggt cgcgctgggc cgcaggggcg gcttgaaggg
     4681 cggcaaggct cgcgcggcgg cgctatcgag caagcgccgt atcgagatcg cgagaggcgc
     4741 cgcgagggcg cgctggaaag catccgcaag cgacgaatga cgctacagta cgagtagcaa
     4801 agccagggcg cgcgcgcagc tccgcggggc atctgcgcag gcccgggaac gcggccgaga
     4861 gtcgcgtcat ccaatgccac gtatgacaaa ccctcaaccg accgccggcc tcgcggccga
     4921 cgcccccgat tccgagcttg cggcgcgcgt cgcggcgggc gagcgcgcgg cattcgaggc
     4981 gctgatgcgc aggcacaacc gcgcgctgtt ccgcacggcc cgcgcgatcc tgcgcgacga
     5041 cgccgaagcc gaggacgcat tgcaggaggc gtatctgcag gcgtatcgag cgatcggcgg
     5101 cttccgggcc gaggcgaagc tcgggacctg gctcgcgcgc atcgtggcga acgaagcgct
     5161 gatgcgcgtg cgcaagcgcg cccggcgcgc cgagatcgtg ccgctgcagg ccggcgtatc
     5221 cgagacggag atcaacgaga tcccggacga gaacatgaac gagacgcccg agcgctcggc
     5281 gcagcgcacc gaaatgcgcc gcttgctcga agcgcagatc gattcgctgc ccgacgacta
     5341 ccgcgtggtg ttcgtgcttc gcgccgtgca ggagatgtcg gtcgaggaaa cttccgaggc
     5401 gctcggcatt ccgccggcca cggtacgcac gcgcctgttc cgcgcgcgca gtctgttgcg
     5461 cgagggactc gccgcgaaga tcgacatcgc ctgcgaagag gcattcagct tcgccggcaa
     5521 gcgctgcgat cgcatcgttg cccatgtctt ggagaggatg aagtgacagc cgtattgaag
     5581 agcgtgcagc tgaaagacgc tgctgacccc gtcgcggacc acgtgcgcgc gatcatcgag
     5641 catctcggcg aagacacgac acgcggcggc ctcgccgcaa cgccggcgcg ctacgcgaag
     5701 gcactgcgct accttgccgg cggctattcc ggcgacctcg aatcctgcat cggcagcggc
     5761 ctgttcccgg ccgaaggcga gagcccggtg ctgatgcgcg acgtccggtt ctattcgctc
     5821 tgcgagcacc atttgctgcc gttccacggc cgcattcacg tcggctacat cccgagccgc
     5881 cgcatcatcg ggctatccaa gatcccgcgc atcgtcgaca tgttcgcgcg gcgctttcag
     5941 gttcaggagc gcctcaccga gcaagtcgcc gatgcgctcg tgcaagcgct cgagccgaag
     6001 ggcgtcgtcg tcgtcagcga ggcgcagcac ctgtgcatgg cgatgcgcgg cgtgcaggag
     6061 cacggcgcga cgacgacggt gcgcgcgacg cgcggggttt gcgagccgct gctcgcgatg
     6121 ctgcaacggg gttgaagcga tgcgttaccg taaattcgtt tgcaacgttt gcggccacgt
     6181 ctacgacgag gcgctcggcg acgcggcagc gggtcttgcc gccggtacgc gctgggaaga
     6241 catccccgaa gactggtcat gccccgagtg cggcgcgacg aaggccgatt tcacgctcct
     6301 cgcggccgaa gccgcggttt cgtagccgct cgcgcggcgc gccgccacgc ggcgcgggtc
     6361 gtcgaatccg ctagcccgat gagcgattgc gccaaccact ggcgattttc gcctccgcgc
     6421 ctccgacccc gcggctcgcg ccgccgcgag cggcgctgtt cgatcaatga cttgcggcgt
     6481 tccggctcgc gtggcacgga agctgcttag cgctcggcga tcgggactag actgagcgcg
     6541 agccgcggca ccacgagagc ggctgtaatg agtcggcgtc ctgcggcgtt tgatcagcga
     6601 ccgggctcga atggaggggt aactcgatgc acttttcgtg gctgtgggtc ttcttcggga
     6661 tgttcttctg gatgctcatg atgcggcccc gccggctttg ggcatgtcag ggacgagggt
     6721 tcaccgacga gcgacgcggc ttcgacgacg agacgcgcgg cccgctcgac cggccttcgc
     6781 gccgcccgcg gcgcgcggat ctcgagagtg agctcgcacg tcgcgatgcg ttgatcgcga
     6841 agctcgagga gcgcgtgcgc gtgctcgagc gcatcgcgac ggacgattcc gcgcggttgc
     6901 gagcggagat cgacgcgcta cgcgacccgc ccgcgagctc cggct
//
LOCUS       DASSNN010000813         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1358285, whole genome shotgun sequence.
ACCESSION   DASSNN010000813 DASSNN010000000
VERSION     DASSNN010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358285"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..642
                     /locus_tag="VFL84_15275"
     CDS             109..642
                     /locus_tag="VFL84_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET7610042.1"
                     /translation="MEQLMVISAVGGEPTAVVQELTRVIMDCGGNIKESRIASFGSDV
                     AMLLLVSGNWHTVSRMERELTKFANSNGVTMQFKRTEPRRFGKELLPYAVDVVGLDQP
                     GVVHSLSGFFAARKVEIGEVSTRSYAATHTGAPMFSVQMAVNIPATVHISGLREEFMD
                     FCDQLNVDAILEPVKHS"
     gene            652..1152
                     /locus_tag="VFL84_15280"
     CDS             652..1152
                     /locus_tag="VFL84_15280"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET7610043.1"
                     /translation="MATPSVGKKIGSFNIATTASPKLTSKDLLGTPYVVYFYPKDDTP
                     GCTIEGKDFRDRHADFGKLGVRVIGVSRDSLASHEKFQGKYALPFELASDADETLCRQ
                     FDVIKDKNMYGKKVRGIERSTFLVDGDGVLRREWRKVKVDGHVAEVLAAAAELTAGTD
                     YPRKGR"
     gene            1197..2642
                     /locus_tag="VFL84_15285"
     CDS             1197..2642
                     /locus_tag="VFL84_15285"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET7610044.1"
                     /translation="MRRWLTFAAAALLIPVLAEAAELPDFGSPADAALSKSREAQIGR
                     GVMLQLRNAGVIVDDPFLEEYVSTIGSRLATHVNDGDFNFNFFVVQDDAINAFALPGG
                     YIGVNSGLLLASESEDELAGVLAHEISHVTQRHLARGALDNQRTSIVSLAAMLAALVL
                     GAASDMPSDAMTGIVAASQGLAAQRQINFTRANESEADRVGIELMSGAGFDPNGMSSF
                     FEKLSRRYGGTSDIVPEILRTHPVSTERVAEARDRVRQLPAATHTSSPQYALAKSRLI
                     VLSAARPEEAFGQFRDKADSNLPEDRYGLALASMRMSLYDNAEALFRGLVADYPSIMA
                     FRVGQAEAMAASGATQPALGLYADAIRLFPRNGPLTTSYAEALIAAGKPAEAHEVLLD
                     LLNNVPPTASQLRLIARAANAEGDVANAYFYMSYYYASIGNLPLAIGQVRMALETPNA
                     NDVDRARVRSRLDQLIEYLPDEQRHLANGPQ"
BASE COUNT          511 a          935 c          906 g          456 t
ORIGIN      
        1 ccttttcgat gcccgcgcgg ggccagtgcg ggcgcggttc gcgagcgttg cgcacccgac
       61 ggttcggggt acactgccgg atcgggttac gccgcgagat tctcttagat ggaacaactc
      121 atggtcatct ccgccgtcgg cggcgagcca accgctgtcg tgcaggagct gacgcgggtc
      181 atcatggatt gcggcggcaa catcaaggag agccggatcg catcgttcgg ctccgacgtc
      241 gcgatgctgc tgctcgtgtc gggcaattgg cacaccgtca gccgcatgga gcgagagctg
      301 acgaagttcg cgaacagcaa cggcgtcact atgcagttca aacgcacgga gccgcgccgc
      361 ttcggcaagg agcttctgcc ctacgccgtc gacgtcgtgg ggctcgacca gcccggcgtc
      421 gtgcacagcc tgagcggctt cttcgcggct cgcaaagtcg aaatcggcga agtctccacg
      481 cgcagctacg ccgcgacgca caccggcgcg ccgatgttct ccgtgcagat ggccgtcaac
      541 atcccggcga cggtccatat ctcgggattg cgcgaggagt tcatggactt ctgcgatcag
      601 ctcaacgtcg acgccatctt ggagccggtc aagcattctt gacttcaggc catggccaca
      661 ccgagcgtcg gcaagaaaat cggctccttc aacatcgcga cgaccgcgag cccgaagctc
      721 acgagcaaag atttgctcgg cacgccttat gtcgtgtact tctacccgaa ggacgacacg
      781 ccgggctgca ccatcgaagg caaggatttt cgcgaccggc acgccgactt cggcaagctc
      841 ggcgttcgcg tgatcggcgt gtcgcgcgat tcgctggcct cgcacgagaa gtttcaaggc
      901 aagtacgctc tgccgttcga gctcgcctcg gatgccgacg agacgctctg ccggcaattc
      961 gacgtgatca aggacaagaa catgtatggc aagaaggtgc gcggtatcga gcgcagcacc
     1021 ttcttagtcg acggcgacgg ggtcttgcgc cgggagtggc ggaaggtcaa ggtagacggg
     1081 cacgtagccg aggttctggc cgccgccgcg gagctcacgg ccggaacgga ctacccgcga
     1141 aaaggtcgat agacggcatg gacggacgcg gccggcaacc acgaagctgt tacgccatga
     1201 ggcgttggct gacgtttgcg gcagccgcgc tgctcatacc cgtgctcgcc gaggccgccg
     1261 agctgcccga tttcggcagc cccgccgatg ccgcgctcag caagagccgc gaagcgcaga
     1321 tcggccgcgg cgtgatgctg cagctgcgca acgccggcgt catcgtcgac gacccgttcc
     1381 tcgaggaata cgtgtcgacg atcggatcgc ggctcgcgac ccacgtcaac gacggcgact
     1441 tcaatttcaa tttcttcgtc gtgcaggacg acgccatcaa cgcgttcgcg ctgccgggcg
     1501 gctacatcgg cgtcaactcg ggcctgctgc tcgcgagcga atccgaggac gagctcgcag
     1561 gcgtgctcgc gcacgagatt tcacacgtca cgcagcggca cctcgcgcgc ggggcattgg
     1621 acaatcagcg cacgagcatc gtgtcgctgg ccgcgatgct cgcggcgctc gtgctcggcg
     1681 cggcgtcgga catgccgagc gacgcgatga cgggcatcgt cgcggcgtcg caagggctgg
     1741 ccgcccagcg tcagatcaac ttcacgcgag ccaacgagtc ggaggccgac cgcgtcggca
     1801 tcgagctgat gtcgggagcc ggcttcgacc cgaacggaat gtcgagcttc ttcgagaagc
     1861 tgagccggcg ttacggcgga acctcggaca tcgttcccga gatcctgcgc acgcacccgg
     1921 tctccaccga gcgtgtcgcg gaagcgcgcg accgcgtgcg ccagcttccc gcggcgacgc
     1981 acacgagcag cccgcagtac gcgttggcca aatcgcgcct gatcgtgctc agcgccgcga
     2041 ggcccgagga agcgttcggg cagttccgcg acaaagccga cagcaacttg cccgaagatc
     2101 gctatggcct cgcgctcgcc tcgatgcgta tgtcgttgta cgacaatgcc gaggcattgt
     2161 tccgcgggct cgtcgcggac tatccgagca tcatggcgtt tcgtgtcggc caggcagagg
     2221 caatggcggc gagcggcgcg acgcagccgg cgctcggctt gtacgcggac gcgattcgcc
     2281 tctttccgcg caacggcccg ctgacgacga gctacgccga ggctctgatc gcggccggca
     2341 agcccgccga ggcccacgag gtgctgctcg atctcctcaa caacgttccg ccgacggcgt
     2401 cgcagctgcg cctcatcgcg cgcgccgcga acgccgaggg cgacgtggcg aacgcgtact
     2461 tctacatgtc gtactactac gcctcgatcg gcaatctgcc gctcgcgatc ggccaagtgc
     2521 gcatggcgct cgagacgccg aacgccaacg acgtcgaccg cgcgcgcgtc cgctcgcgcc
     2581 tcgatcagct catcgaatac ctgcccgacg agcagcgcca cctcgcgaac ggcccgcaat
     2641 aagccttcgg acgacgttcg gttgcggcgg ccttaaatag gactaaaatg gtcccctatc
     2701 aaatcgaccg cgggaaatcg ccgtcatgct gcgcatgagc aaattgaccg attacgggac
     2761 catggtcctg gcccagctgg cggccagcga cgccagctgg accacggc
//
LOCUS       DASSNN010000814         3117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_724376, whole genome shotgun sequence.
ACCESSION   DASSNN010000814 DASSNN010000000
VERSION     DASSNN010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724376"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFL84_15290"
     CDS             complement(<1..241)
                     /locus_tag="VFL84_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET7610045.1"
                     /translation="MALQLGDVAPDFEAQTTAGPIRFHEWLGNHWAVLFSHPRDFTPV
                     CTTELGLTEKLRPEFERRGVKVIGLSVDGVEDHERW"
     gene            336..746
                     /locus_tag="VFL84_15295"
     CDS             336..746
                     /locus_tag="VFL84_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET7610046.1"
                     /translation="MAASDKPMTARQYRALADFRYELRRFLRYSEEVTRRHGLTPLKY
                     QLLLQVKGCGPATIGELAERLQAKHHGMVALVTRCAKAGLVVRGENPDDRRAVRVRLT
                     AKGERALEKLARLHRNELLAIQGRLPFPSFTGLE"
     gene            751..1365
                     /locus_tag="VFL84_15300"
     CDS             751..1365
                     /locus_tag="VFL84_15300"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET7610047.1"
                     /translation="MYRLPSLPYELEALAPVIGAETMRTHHGKHHARYVQVTNEIAGA
                     TQPPLEELVADARRRGDAKLFNNAAQAWNLAFFWESMAPAATSPTGELARAIDAAFGS
                     LAGLRQEFVAKGAAQFGSGWVWLVATNGGKLEVVTSHDADQPWLGALGAPLLVCDVWE
                     HAYYLDYKNERGRFLEAWFDRLANWAFAATQLTDHRKRYRYPPP"
     gene            complement(1557..>3117)
                     /locus_tag="VFL84_15305"
     CDS             complement(1557..>3117)
                     /locus_tag="VFL84_15305"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7610048.1"
                     /translation="ALGASRGRIGRQFFIESILLAAFGALAGLLLARLLLDAAAALLP
                     DPEVFFRTAMAPGAPRIAGAPGLTRIGAEMIGLDAATLLFTLSVTIVTAGLVGIVPAL
                     KASALRPIEALKGGGTAVGSRGRRDFGARATPLIAQIALSLVLLAGAGLMVRSAAALY
                     GTEIGVDAANLVTVSFNLPEASYGSGPDSDRGRAFYAQLMERVRALPGIDSAGLGSCA
                     PISGGCNGTRIEFIEPHRDGDGLIGVLWATPDYFATLGLALLEGRGFSDADRAGQPRV
                     VLINEAAARAYWPNESPLGKTVGVWQGGFQEGAEVIGVVADARYREIGAAAAPDVYLP
                     LAQSYRSGMYLFVRSRLPLQSLVNSLSGEVRALDPTLPLAGVKTMETRFGEAMWRTRV
                     GAWLLSAFAGLALLLTAIGIFGVTAQAAAQRTAEIGVRMALGADPRDLLRWMLRQAAL
                     VTAAGLALGVACALALTRLIGAMLYGVEVYDPLTFGAVAALLGLVVLAAAYVPAKRAT
                     RVDVLVALRSE"
BASE COUNT          552 a         1048 c         1052 g          465 t
ORIGIN      
        1 cccagcgctc gtgatcctcg acgccgtcta ccgacaggcc gatgactttg acgccgcgcc
       61 gttcgaactc tggccgcagc ttctccgtga gcccaagctc cgtcgtgcag acgggcgtga
      121 aatcgcgcgg atgagagaac agcacggccc aatgattgcc gagccactcg tgaaaacgaa
      181 ttgggcccgc cgtcgtctga gcctcgaagt ccggcgccac gtccccgagt tgcaatgcca
      241 tggtgtgctc ctgattcctg gatgggacga gtaccgtaac acctcacaat gtgatataaa
      301 aaaatccgtt cgcactatca atatgagcat tggctatggc ggcttcggac aagccgatga
      361 ccgcgcggca ataccgcgcg cttgcggatt ttcgctacga gctccggcgg ttcttgcgtt
      421 atagcgagga agtcacgcgg cgccatggct tgacgccgct gaagtatcag ttgctgctgc
      481 aagtgaaagg gtgcgggccg gccacgatcg gcgagctcgc ggagcgcttg caggcgaagc
      541 accacggcat ggtggcgctc gtcacgcgct gcgcgaaggc gggtttggtc gtgcgcggtg
      601 aaaacccgga cgacaggcgc gccgtgcgcg tgcggctcac cgccaaaggc gaacgcgcgc
      661 tcgagaagct ggcgcggctg caccgcaacg agctgctcgc cattcagggc cggctaccgt
      721 tcccgagttt cactggtctg gagtagacaa atgtatcggc ttccgtccct tccttatgag
      781 ctcgaagcgt tggccccggt catcggcgcc gagacgatgc gcacgcacca tggcaagcac
      841 catgcacgct acgtgcaggt cacgaacgag atcgcgggtg cgacgcagcc gccgctcgag
      901 gagctcgttg ccgacgcgcg ccgccgcggc gatgcgaagc tcttcaacaa cgccgcgcag
      961 gcttggaatc tcgcgttctt ctgggagtcg atggcgcccg cggcgacgtc gcccaccggc
     1021 gagctcgcgc gcgcaatcga cgccgccttc ggcagccttg cgggcttgcg gcaggaattc
     1081 gtggcgaaag gtgccgcgca gttcggctcc ggctgggtat ggctcgttgc gacgaacggc
     1141 ggcaagctcg aggtcgtgac ctcgcacgat gcggaccaac cgtggctcgg cgcgctcggc
     1201 gcgccgctgc tggtctgcga cgtctgggag cacgcgtact atctagacta caagaacgaa
     1261 cgcggccggt ttctcgaagc atggttcgac cggctcgcga actgggcttt cgcggcgact
     1321 cagctcacgg accaccggaa gcgctaccgc tatccgccgc cgtgacggcg atggctgctt
     1381 cggcggccag aaaatcgagc gtatctgtcg ttccgcccga ccgaggcgac ggccaaggac
     1441 acgctcgagt ggttccgctc gttgcccgag gaccccggac cgccgtgcag agaatcttgg
     1501 agcgcgaagc tcaggtcatc gcggcatgga gggcgcgggc ctaggacagc gccgaatcat
     1561 tcgcttctga gcgcgacgag cacgtccact cgcgtcgcgc gctttgcggg aacgtaagcc
     1621 gccgcgagca ccacgagccc gagcagcgcg gcgacggcac cgaaggtgag cggatcgtag
     1681 acctccacgc cgtagagcat cgccccgatc aggcgcgtca gcgcgagcgc gcacgcgacg
     1741 cccagtgcga gccccgccgc ggtaacgagc gccgcctgac gcagcatcca gcgcagcaag
     1801 tcgcgcggat cggcgccgag cgccatgcgc acgccgattt cggccgtgcg ctgcgccgcc
     1861 gcttgcgccg ttacgccgaa gataccgatt gccgtgagaa gcagcgcgag cccggcgaac
     1921 gcagacagca gccaggcgcc gacacgagtg cgccacatcg cctcgccgaa ccgcgtttcc
     1981 atggtcttga cgccggcgag cggcagggtt ggatcgagag cccgcacttc gcccgacaac
     2041 gagttgacga ggctctgcag cggcaggcgg ctgcgcacga acagatacat gcccgagcga
     2101 tacgactggg cgagcgggag gtacacgtcg ggagcggccg cggcgccgat ctcgcggtaa
     2161 cgggcatccg cgaccactcc gatgacttcg gcgccctcct ggaatcctcc ttgccacact
     2221 ccgaccgtct tgccgagcgg cgattcgttc ggccaataag cgcgcgcggc ggcctcgttg
     2281 atcagcacga ctcgcggttg ccctgcacgg tcagcgtcgc tgaagccgcg accttcgagc
     2341 agcgcgagcc cgagcgtcgc gaagtagtcg ggcgtagccc agagcacgcc gatgagaccg
     2401 tcgccgtcgc ggtgcggctc gatgaactcg atacgcgtgc cgttgcagcc acccgatatc
     2461 ggcgcgcaac tacccaggcc ggccgagtcg atgccgggta acgcgcgcac gcgttccatg
     2521 agctgcgcgt agaacgcgcg acctctgtcg gagtcgggtc cggagccgta gctcgcctcg
     2581 ggcaagttga agctcacggt aaccaagttt gcggcgtcga ctccgatctc cgtcccatag
     2641 agcgccgcgg cgctcctgac catgaggcct gcgccggcga gcagcacgag cgacagcgcg
     2701 atttgcgcga tcaacggcgt ggcgcgagcg ccgaagtcgc gacggcctcg cgagcccacg
     2761 gcggtgcccc cgcccttcag cgcctcgatc ggacgcaacg cggaggcttt cagagcaggc
     2821 acgatcccga ccagaccggc ggtcacgatc gtgacgctga gcgtgaacaa cagcgtagcc
     2881 gcgtcgaggc cgatcatttc ggcgccgatg cgagtgaggc ccggcgcgcc cgcgatccgt
     2941 ggcgcgcccg gcgccatcgc cgtgcggaag aacacctcgg gatcgggaag cagggccgcg
     3001 gcggcgtcca ataggagccg ggcgaggagc aatcccgcaa gcgctccgaa tgcggcgagc
     3061 aggatgcttt cgatgaagaa ctgccggccg atgcggccgc ggctcgcgcc gagcgcg
//
LOCUS       DASSNN010000815         9294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_724725, whole genome shotgun sequence.
ACCESSION   DASSNN010000815 DASSNN010000000
VERSION     DASSNN010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724725"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VFL84_15310"
     CDS             <1..814
                     /locus_tag="VFL84_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HET7610049.1"
                     /translation="LYSAAAGGGISIADLFAAGVVPAILLMISVMLTAYVIARRRGYA
                     TAQFPGMRTVAMSFVAALPGLLLVLVIFGGIRAGIFTAVESASVAVLYAVVVTALAYR
                     RLDFRQFKTACASAAHTAGIILFVISAAATFGWLLAYLEVPAQVVAGVRELADDATTI
                     LIAMNVVLLVLGTFMDMAPLIIICTPIFLPVATAFGVSPLQFGVMLILNAGIGLITPP
                     VGSVLFVGTAIGKISIAETMRTIWPFYLAGLVVLALVTLWPALSLWLPGVLR"
     gene            811..1482
                     /gene="eda"
                     /locus_tag="VFL84_15315"
     CDS             811..1482
                     /gene="eda"
                     /locus_tag="VFL84_15315"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET7610050.1"
                     /translation="MTAARSLTEMLRAQRIVAVVTIERATDALPLADALCAGGVRVVE
                     ITLRSDAARSAIEAIAKARTDVIVGAGTVRDARSLRAARDAGAQFVVSPGFTPALHAA
                     AAAAALPWLPGVATASEILLAQEAGHSLLKFFPAEAAGGPRALRAFAAVFPEVRFCPT
                     GGITAENLGGYLELANVRCVGGTWLAPPALVAAKQWQQIKELAAQAAALAGASAVPGD
                     RPVLS"
     gene            complement(1479..4217)
                     /locus_tag="VFL84_15320"
     CDS             complement(1479..4217)
                     /locus_tag="VFL84_15320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7610051.1"
                     /translation="MSRRAPESLPQRSIISAAVALGLAALPLVGAAQEVGDEIIVRGY
                     RSSLNASLSAKRESAGAVDVIVAEDIADFPDLNLAEALQRIPGVAISRAGGEGRQVTV
                     RGLGPTFTTTRINGMEALSTGGFTDALGGQNRTRGFDFNAFDSELFTRLAVRKTSSAE
                     IEEGGLGATLDLRTARPFDYDGFTIATSAQLGYNDRSEESDPRAGFQISNTFADDKFG
                     ALLSLSWSERNTLDEGSSTVRWSDTENFGSVNGVPLLPAAVATHEVNTAFHPRLPRTD
                     SYVQNTERFGVAGSLQFRPSEATSIDLDVLVSQADTTRDEAFIQAALNNNGFVTATNI
                     SNYSIRDAAIVSATLTNATLLSERRHDELAVDFDQTVLSFRHDFNDRLNVSALVGSAD
                     SEFDNPVQRYVILQKNGDFSYDMTGPDGAGFVWGPQTRDLNGWTVSNFRKREPFTLNE
                     LDVGELTFGFRFNDLLTLNAGVASKTYNLETSQAVMATETNQGITGAQPATLLYTYDA
                     GTLGSWAAPNQDAFDQQYGFYADSGAFTTSTTFTPALPNSFTVEEQTDSAFVQLDFAF
                     GDRVPIRGNVGVRSFETDQNSTGISNLATGTISADFQYSDTLPSLNMVFELTDQLLLR
                     FGYAEVINRPGMQSLRPVATVSVAGSNRSVNGNNPGLGPTLADTYDLSAEWYFAPESV
                     LALALFQKDIGSFVQTIVQSVPYTETGLPLQQAIDACNGSPSGYGPNCNENLPWNVTA
                     PGNSPGGDLKGYEISYQQPFSFFDGFARNFGIMANYTYVDSKIDYLGVVGGVTTVVRP
                     DESLTNLSKNMANLTFYFENEKLGARISLAERDDYLTSIPGRNGTYVERTEGTRNVDI
                     SANFNFNDNLKLTFEGLNLTDESENQRLDATILPANVVSYFHETGRQFYLGLRYTL"
     gene            complement(4442..6208)
                     /locus_tag="VFL84_15325"
     CDS             complement(4442..6208)
                     /locus_tag="VFL84_15325"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7610052.1"
                     /translation="MTNADAGTSRASRLAAALAAALLLAACTPPDTSLVPPPAVGWPT
                     NGGNWYNQRYSPLTAIDRGNVANLKGVWHTRLEGSGVGPQYSGEAQPLVVDGVIYVPT
                     GASDLFALDVATGEILWSYKAGLDPAIDVVCCGWSSRGVGHGNGRIFLGRLDGQLVAL
                     DAKTGAVAWSVQAERWQDGFSITSAPLYYDGLVITGFSGAELGVRGRVKAFHASDGEL
                     AWTFYTIPARGEPGNETWPQDNELWQHGGATVWHTPAVDPELGLIYFSTGNPGPDFNG
                     AVRAGDNLYTSSIMAVELETGRYRWHFQQVHHDIWDYDSPAPVVLFDLEYQGRMRKGL
                     AQPSKTGWVYILDRTDGTPLVGIDEKPVPQEPRQATAATQPYPRGDAFVPQHLDIAPE
                     GYPLVNGGKIFTPYWTDYIIAKPGILGGGNWPPAAYDPTTGYLYVCASDQASSFVAAE
                     LGTERPPAGEYFVGGAFGTNPLAPYGTFTALDMRTNKIVWQQHWADRCYSGATATAGG
                     LVFVGRNDGRLTALDSSDGTKLWEFQTGAGMNAPVSVFAHDGKQYVVAYSAGNMFAAS
                     AKGDNVWLFALDGTLGPVPPAR"
     gene            complement(6243..7079)
                     /locus_tag="VFL84_15330"
     CDS             complement(6243..7079)
                     /locus_tag="VFL84_15330"
                     /EC_number="4.1.2.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019822412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxycinnamoyl CoA hydratase/lyase"
                     /protein_id="HET7610053.1"
                     /translation="MARKKDPWGKLVRVDFDDGIAWVTLNRPDKRNAMSPSLNAEMRD
                     TVEELATDERCGVFVLTGAGDSFAAGMDLKEYFRETDGKPDMILKVRRTAEEWQWRRL
                     RDFPKPTIAMVNGWCFGGAFTPLVACDLAIAADEATFGLSEVNWGIIPAGNVTKATSE
                     TIGLRNALFYAMTGEPFKGKKAAEMGLVNESVPRKKLKARTTEIARMLLTKNQAVLRG
                     IKIAMKRMRYMDWDASADYLYAKMGEALFFGASDNRESAMKAFLDDKTFKPGLEHFKG
                     TK"
     gene            7218..>9294
                     /locus_tag="VFL84_15335"
     CDS             7218..>9294
                     /locus_tag="VFL84_15335"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HET7610054.1"
                     /translation="MAYELLGKNFVPADVHGKVTGKAKYAEDFRAEGMVFCKLLLSPM
                     PHARVRNVDTSAAEALPGVRGILRAADVTKVPPPGEPILTDEPLWIGQPILAVAADSE
                     QIAEDAIDLIKVDFEELPFTVDPLQSLFPGGPDARSDGNTVDNRLIGPPKLKQVKWQA
                     GDFTRDGEDKLPTGEPLVEWSYGDVEAGFKKAKLVLDETFVTAGMSHHSMEPRSAMAY
                     WENGKCFVYGSTQSQSFVVPGLANLIGITPDDLVYVAETCGGGFGSKGTEYPVMSIPA
                     HMSKKIGRPVMLRISRAEEYYFGYARTGFQGRIKLGFDERGRVTAADLYTVQENGATT
                     GFPDWPSSGETVSILYQPEAMRFRGMCVMTNTVPRSAQRGPGHNQTVAAVEPLIDKAA
                     KELGLDRLAIRRLNGPRMNAKLGENQGPVTSCYLQEAFDKAASLFNYEERRARSGRKN
                     GTKVTGIGVGQAFHPAGFAGFDGLVRITTDGKVHLHSGVGNLGTYSHTGTSRIAAEVL
                     KANWDNCVVERGDTRKGLPWNIGQFGSNTSFTMARTNFVAATDAVNKLKEIAAMDLGG
                     APADYDIGGERVFSKADPSKGLTYAQAAQRAVELGGKFDGHETPMDINPMTAAAAQSL
                     AGTGLVGVAKDNLPLTASPAAFVAGFAEIELDTETGAFSIIDYVAVADCGTVIHPMGL
                     ATQIKGGAVM"
BASE COUNT         1446 a         3030 c         3190 g         1628 t
ORIGIN      
        1 cctgtattcg gcagcggcgg gcggcgggat ttcgatcgcc gatctctttg ccgccggcgt
       61 ggtaccggcg atcttgctca tgatcagcgt gatgctgacc gcttatgtca tcgcgcggcg
      121 gcgcggctat gcgacggcac agttccccgg gatgcgcacc gtcgcgatga gcttcgtggc
      181 cgcgctgccg gggctcctgc tcgtgctcgt gatcttcggc ggcattcgcg ccggcatctt
      241 cacggccgtc gagagcgcga gcgtcgcggt gctgtatgcg gtcgtcgtga cggcgctcgc
      301 gtatcggcgc ctcgacttcc gccagttcaa gaccgcctgc gcgagcgccg cgcacaccgc
      361 gggcatcatc ctgttcgtga tcagcgccgc cgcgacgttc ggttggctgc tggcgtatct
      421 cgaggtgccg gcgcaagtcg tcgccggtgt tcgcgagctg gccgacgacg cgacgacgat
      481 cttgatcgcc atgaacgtcg tgctgctcgt gctcggcacg ttcatggata tggcgccgct
      541 gatcatcatc tgcacgccga tcttcttgcc cgtggcgacg gcattcggcg tgagcccgct
      601 gcaattcggc gtcatgctga tcttgaacgc aggtatcggc ctcatcacgc cgcccgtcgg
      661 ctcggtcctg ttcgtcggga ccgcgatcgg caagatcagc atcgcggaaa cgatgcggac
      721 gatctggccg ttctatctgg cgggcctggt cgttctcgcg ctggtcacgc tgtggcccgc
      781 gttgtcgttg tggctgccgg gcgtgctgcg atgaccgcgg cacggtcgct gaccgaaatg
      841 ctccgcgcgc agcgcatcgt cgccgtcgtc acgatcgagc gtgcgaccga cgcgttgccg
      901 ctcgccgacg cgctttgcgc gggcggcgtg cgcgtcgtcg aaatcacgtt gcgcagcgac
      961 gcggcgcgct cggccatcga agcgattgcc aaagcgcgca ccgacgtcat cgtcggcgcc
     1021 ggcaccgttc gcgacgcgcg cagcttgcgc gccgcgcgcg acgcgggcgc gcaattcgtc
     1081 gtgagccccg gattcacgcc cgcgctgcac gcggctgcgg ccgcggcggc cctgccgtgg
     1141 ctgccgggcg tcgcgacggc gtccgagatc ttgctcgcgc aggaggccgg gcattcgctg
     1201 ctgaagttct ttccggcgga agcggccggc ggcccgcgcg cgctgcgcgc attcgccgcc
     1261 gttttccccg aggtccgctt ctgcccgacg ggcggcatca cggccgagaa cctcggcggc
     1321 tacctcgagc tcgcgaacgt ccgctgcgtc ggcggcacgt ggctcgcgcc gccagcgctc
     1381 gtggccgcca agcagtggca gcaaatcaag gagctggccg ctcaggcggc cgccctcgcc
     1441 ggcgcttccg ccgtccctgg cgatcggccc gtcttgagct agagcgtgta gcgaaggccc
     1501 aggtagaact gcctgccggt ctcgtggaaa tacgagacga cgttcgcggg cagtatcgtc
     1561 gcatcgagcc gctggttctc cgactcgtcc gtgaggttga gcccctcgaa cgtgagcttc
     1621 aggttgtcgt tgaagttgaa gttcgcggag atgtcgacgt tcctcgtgcc ttcggtgcgc
     1681 tcgacgtacg tgccgttcct gcccgggatg ctcgtcaagt agtcgtcgcg ctccgcgagc
     1741 gagatgcgcg cgccgagctt ttcgttctcg aagtagaagg tcaggttcgc catgttcttc
     1801 gacaagttcg tcagcgactc gtcgggacga acaacagtcg tgacgccgcc gacgacgcct
     1861 aggtagtcga tcttcgagtc gacgtacgtg tagttcgcca tgatgccgaa attgcgcgcg
     1921 aatccgtcga agaacgaaaa cggctgctgg tacgagatct cgtacccttt gaggtccccg
     1981 ccggggctgt tgccgggggc ggtgacgttc cagggcagat tttcgttgca gttcggcccg
     2041 tagccggacg ggctgccgtt gcaagcgtcg atcgcttgct gcagcgggag acccgtctcc
     2101 gtgtacggca cgctttgcac gatcgtctgc acgaagctgc cgatgtcctt ctggaacagc
     2161 gcgagcgcga gcaccgactc cggcgcgaag taccactcgg cggacaggtc gtaggtatcg
     2221 gccagcgtcg gcccgagacc cgggttgttg ccgttcacgc tgcggttgct gccggccacc
     2281 gagaccgtcg cgacgggccg cagcgactgc atgccgggtc ggttgatgac ctcggcataa
     2341 ccgaagcgaa gcagcagctg gtccgtgagc tcgaacacca tgttcaacga cggcagcgtg
     2401 tcggaatact ggaagtcggc gctgatcgtg cccgtagcga ggttcgagat gcccgtcgag
     2461 ttctgatcgg tctcgaacga gcgcacgccg acgttgccgc ggatcggcac gcggtcgccg
     2521 aaggcgaagt cgagctgcac gaacgcgcta tcggtttgtt cctcgaccgt gaacgagttc
     2581 ggcagcgcgg gcgtgaaggt ggtgctcgtc gtgaacgcgc cgctgtcggc gtagaagccg
     2641 tactgctgat cgaacgcgtc ctggttcggc gccgcccacg agccgagcgt gcccgcgtcg
     2701 taggtgtaga gcagggtggc cggctgcgcg ccggtgatgc cctggttcgt ctcggtcgcc
     2761 atgaccgcct ggctcgtttc gaggttgtac gtcttgctcg ccacgccggc gttcagggtc
     2821 agcaaatcgt tgaagcggaa cccgaacgtc agctcgccga cgtcgagctc gttcagcgtg
     2881 aacggctcgc gcttgcggaa gttcgacacg gtccacccgt tcaggtcgcg cgtctggggc
     2941 ccccatacga agcccgctcc gtcggggccg gtcatgtcgt agctgaagtc gccgttcttc
     3001 tgcaggatca cgtagcgctg cacggggttg tcgaactcgg aatccgcgct gccgacgagc
     3061 gcgctcacgt tgagccggtc gttgaagtcg tggcggaacg acagcacggt ctggtcgaag
     3121 tcgacggcga gctcgtcgtg gcggcgctcg gaaagcagcg tcgcgttcgt cagcgtcgcg
     3181 gacacgatcg cggcgtcgcg aatcgaataa ttggagatgt tcgtcgcggt gacgaacccg
     3241 ttgttgttca gcgccgcctg gatgaacgcc tcgtcgcgcg tcgtgtcggc ctgcgagacg
     3301 agcacgtcga gatcgatcga tgtcgcctcg ctcggccgga actgcagcga gccggccacg
     3361 ccgaaacgct cggtgttctg cacgtaggaa tcggtacgcg gcaagcgcgg gtgaaaggcc
     3421 gtgttgacct cgtgcgtggc aacggctgcg gggagcaggg gaacaccgtt cacggagccg
     3481 aaattttcgg tgtccgacca gcggaccgtg ctgctgcctt cgtcgagcgt gttgcgctcg
     3541 ctccacgaca acgagagcag tgcgccgaac ttgtcgtcgg cgaacgtgtt gctgatctgg
     3601 aaaccggcgc gcggatcgct ttcctcggag cggtcgttgt aaccgagctg cgccgacgtt
     3661 gcgatcgtga agccgtcgta gtcgaacggc cgcgcggttc gcaagtcgag agtcgcgccg
     3721 aggccgcctt cctcgatctc ggccgacgac gtcttgcgga cggcgagccg cgtgaacagc
     3781 tccgagtcga acgcattgaa gtcgaagccg cgcgtgcggt tctgcccgcc gagcgcgtcc
     3841 gtgaagccgc ccgtcgacag cgcctccatg ccgttgatgc gcgtcgtcgt gaatgtgggc
     3901 ccgaggccgc gaacggtgac ctgccggcct tcaccgcccg cgcgcgagat cgcgacgccg
     3961 gggatgcgct gcagcgcttc cgcgaggttc agatcgggga aatcggcgat gtcctcggcg
     4021 acgatgacgt cgacggcgcc ggccgattcg cgcttcgcgc tcaacgatgc gttgaggctg
     4081 ctgcggtagc cgcgcacgat gatttcgtcg ccgacttcct gagcggcgcc gacgagcggc
     4141 aaagccgcga gccccaaggc gacagcggcg ctgatgatcg aacgctgggg caacgactcg
     4201 ggcgcccggc gggacatgac actgctcata cgtgatcccc cttgaactgg tgagcgacga
     4261 tgccgtccgg attggcgttg cggcggtgcg gacccgccga atgccaccgg tatcattttc
     4321 ctcggatgtc taccggtgtc atcgcgatac tactcccgcg ccgaggcgat gggcaagggg
     4381 gagccgaccg tggcagaggt ttgttgcgcc gcgcccacaa cgcgggagcg gaacgcttgc
     4441 ttcagcgtgc gggaggtacc gggccgagcg tgccgtcgag cgcgaacagc cagacgttgt
     4501 cgcccttcgc ggaggccgcg aacatgtttc cggccgagta cgcgacgacg tattgcttgc
     4561 cgtcgtgcgc gaacacgctg acgggcgcgt tcatgccggc gccggtctga aactcccaga
     4621 gcttcgtgcc gtcgctcgag tcgagcgccg tgagccggcc gtcgttgcgg ccgacgaaca
     4681 cgaggccgcc cgccgtcgcc gtcgcgccgc tatagcagcg gtcggcccag tgctgctgcc
     4741 agacgatctt gttcgtgcgc atgtcgagcg ccgtgaacgt gccgtaaggc gcgagcggat
     4801 tagtcccgaa agcgccgccg acgaagtact cgcctgccgg cggccgctcg gtgccgagct
     4861 ccgcggccac gaacgagctc gcctgatcgc tcgcgcacac gtagaggtag cccgtcgtcg
     4921 ggtcgtacgc ggccggcggc cagttgccgc cgcccaagat cccgggcttc gcgatgatgt
     4981 agtcggtcca atacggcgtg aagatcttgc cgccgttcac gagcggatag ccttcgggcg
     5041 cgatgtcgag atgctgcggc acgaacgcat cgccgcgcgg gtatggctgc gtcgcggccg
     5101 tcgcttggcg cggctcctgc ggcacgggct tctcgtcgat gccgacgagc ggcgtgccgt
     5161 ccgtgcgatc gaggatgtag acccagcccg tcttgctcgg ctgcgccagt cccttgcgca
     5221 ttcggccttg gtactcgagg tcgaacagca cgacgggggc ggggctgtcg tagtcccaga
     5281 tatcgtgatg gacttgctgg aagtgccagc ggtagcgtcc ggtctcgagc tcgacggcca
     5341 tgatcgacga cgtgtagagg ttgtcgccgg cgcgcacggc gccgttgaag tcggggcccg
     5401 ggttgcccgt cgagaagtag atgaggccga gctcgggatc gacagccggc gtgtgccaga
     5461 ccgtcgcgcc gccgtgctgc cagagctcgt tgtcctgcgg ccacgtctcg ttgccgggct
     5521 cgccgcgcgc gggaatcgta tagaacgtcc acgcgagctc gccgtcgctc gcgtggaacg
     5581 ccttcacgcg gccgcgcacg ccgagctcgg cacccgagaa gcccgtgatg acgagaccgt
     5641 cgtagtagag cggcgcgctc gtgatcgaga agccgtcttg ccagcgctcg gcttgcacgc
     5701 tccaggcgac ggcgcccgtt ttcgcgtcga gcgcgacgag ctggccgtcg agcctgccca
     5761 agaagattcg gccgttgccg tgcccgacgc cgcggctgct ccagccgcag caaacgacgt
     5821 cgatcgcggg atcgaggccc gccttgtacg accagaggat ctcgccggtc gcgacgtcga
     5881 gcgcgaagag atcgctggcg ccggtcggca cgtagatgac gccgtcgacg acgagcggct
     5941 gcgcctcgcc ggaatactgc gggcccacgc ccgagccttc gagccgcgtg tgccagacgc
     6001 ctttgagatt ggcgacgttg ccgcgatcga tcgcggtcag cggcgaatag cgttggttgt
     6061 accagttgcc gccgttcgtc ggccagccga cggccggcgg cgggacgagc gaggtgtcgg
     6121 gcggcgtgca cgccgcgagc agcagcgccg ccgcgagcgc cgcagcgagg cggctcgctc
     6181 gacttgtccc agcgtccgcg ttcgtcatcg ccagctcccc ccgaagccgg tgcatcgcga
     6241 ggttacttcg tgcctttgaa gtgctcgagc cccggcttga acgtcttgtc gtcgagaaac
     6301 gctttcatgg cgctctcgcg gttgtcgctt gcgccgaaga acagcgcttc gcccatcttc
     6361 gcgtacagat aatccgcgga cgcgtcccaa tccatgtagc gcatccgctt catcgcgatc
     6421 ttgatgccgc gcagcaccgc ctgattcttc gtgagcaaca tgcgcgcgat ctcggtcgtg
     6481 cgcgccttga gcttcttgcg gggtaccgac tcgttgacga gccccatctc ggctgctttc
     6541 ttgcccttga acggctcgcc cgtcattgca tagaagagcg cgttgcgcag cccgatcgtc
     6601 tcggacgtcg ccttcgtgac gttgccggcc gggatgatgc cccagttcac ctccgacagg
     6661 ccgaacgtcg cctcgtcggc cgcgatcgcg agatcgcacg cgacgagcgg cgtgaacgcg
     6721 ccgccgaagc accagccgtt gaccatggcg atcgtcggct tcgggaaatc gcgcaggcgg
     6781 cgccactgcc attcctcggc cgtgcgccgc accttcaaga tcatgtcggg tttgccgtcg
     6841 gtctcgcgga agtattcctt gagatccatt ccggccgcga acgagtcgcc ggcgcccgtc
     6901 agcacgaaga cgccgcagcg ttcgtcggtc gcgagctcct cgacggtgtc acgcatctct
     6961 gcgttgaggc tcgggctcat tgcgttgcgt ttgtcgggac ggttcaacgt tacccacgcg
     7021 atgccgtcgt cgaagtcgac gcgcacgagc ttgccccagg ggtccttctt gcgggccatg
     7081 cggaaatctc ctaaagaatc aatgctgcgg cttaaaattg ctgttgttgt ccggttgccg
     7141 ggtcgccgcc ttgggcttaa agtccacgag tcgccggacc ggcggttcgg cgcgcagttt
     7201 atcaaggagt tcgcgcgatg gcttacgagt tgttgggaaa gaacttcgtg ccggccgacg
     7261 tgcacggcaa ggtgaccggc aaggccaagt acgcggaaga cttccgcgcc gagggcatgg
     7321 tgttctgcaa gctgctgctg tcgccgatgc cgcacgcgcg cgtgcgcaac gtcgatacga
     7381 gcgcagccga ggcgctgccg ggcgtgcgcg gaatactgcg agcggcggac gtcacgaaag
     7441 tgccgccgcc cggtgagccg atcctgacgg acgagccgct gtggatcggc cagccgattc
     7501 tcgcggtcgc cgccgacagc gagcagatcg ccgaggacgc catcgatctc atcaaagtcg
     7561 acttcgagga gctgccgttc acggtcgatc cgctgcagag cctgttcccg ggcggccccg
     7621 atgcgcgcag cgacggcaac accgtcgaca accggctgat cggcccgccg aagctgaagc
     7681 aagtcaaatg gcaggccggc gatttcacgc gcgacggcga agacaaattg ccgacgggcg
     7741 agccgctcgt cgaatggtcg tacggcgacg tcgaagccgg cttcaagaaa gcgaagctcg
     7801 tgctcgacga aacgttcgtg acggccggca tgtcgcacca ttcgatggag ccgcgctcgg
     7861 cgatggcgta ctgggagaac ggcaagtgct tcgtgtacgg ctcgacgcag agccagagct
     7921 tcgtcgtgcc gggcctcgct aacctgatcg gcatcacgcc ggacgatctc gtctacgtcg
     7981 ccgagacgtg cggcggcggc ttcggctcga agggcaccga atatcccgtg atgtcgatcc
     8041 cggcgcacat gtcgaagaag atcggccggc ccgtcatgct gcgcatcagc cgcgccgagg
     8101 agtactactt cggctacgcg cgcacgggtt tccagggccg catcaagttg ggtttcgacg
     8161 agcgcggccg cgtgacggcc gcggatctct acaccgtgca ggagaacggc gcgacgacgg
     8221 gcttccccga ttggccgtcg tcgggcgaga ccgtctcgat cctctatcag ccggaagcca
     8281 tgcgcttccg cggcatgtgc gtcatgacga acaccgtgcc gcgctcggcg cagcgcggcc
     8341 cgggccacaa ccagaccgtc gcggccgtcg agccgttgat cgacaaagcc gcgaaggagc
     8401 ttgggctaga taggctcgcg atccgccgct tgaacggccc gcgcatgaac gcgaagctcg
     8461 gcgagaacca gggccccgtc acgagctgct atctgcaaga ggcgttcgac aaggcggcgt
     8521 ctctcttcaa ctatgaggag cgcagggcgc gcagcggccg caagaacggc acgaaggtca
     8581 ccggcatcgg cgtcggccag gcgtttcatc cggccgggtt cgccggcttc gacgggctcg
     8641 tgcgcatcac gacggacggc aaggtgcact tgcacagcgg tgtcggcaac ttgggcacgt
     8701 actcgcacac gggcacgtcg cgcatcgcgg ccgaagtgct gaaagcgaac tgggacaact
     8761 gcgtcgtcga gcgcggcgat acgcgcaagg gcttgccgtg gaacatcggc cagttcggca
     8821 gcaacacgtc gttcacgatg gcgcgcacga acttcgtcgc ggccaccgac gcggtcaaca
     8881 agctcaagga aatcgccgcg atggatttgg gcggcgcgcc cgccgattac gacatcggtg
     8941 gcgagcgcgt gttctcgaag gccgatccga gcaagggcct cacgtatgcg caggccgcgc
     9001 agcgcgccgt cgagctcggc ggcaagttcg acgggcacga gacgccgatg gacatcaacc
     9061 cgatgacggc ggccgcggcg caatcgctcg cgggcacggg cctcgtcggc gtcgcgaagg
     9121 acaacctgcc gctgacggcg tcgcccgcgg cgttcgtcgc cggcttcgcc gagatcgagc
     9181 tcgacaccga gacgggcgcg ttcagcatca tcgactacgt cgccgtcgcc gactgtggca
     9241 ccgtgatcca tccgatgggt ctcgcgacgc agatcaaggg cggtgccgtc atgg
//
LOCUS       DASSNN010000816         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1739730, whole genome shotgun sequence.
ACCESSION   DASSNN010000816 DASSNN010000000
VERSION     DASSNN010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739730"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..2062)
                     /locus_tag="VFL84_15340"
     CDS             complement(95..2062)
                     /locus_tag="VFL84_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HET7610055.1"
                     /translation="MQPNLPPQLTPRAIVLAIVLAMILAAANTYLGLFAGLTIASAIP
                     AAVISMAVLRVLGGGGILENNIVQTGGSAGASLASGVIFTIPALVILGFWDDFDFLWV
                     VAIAALGGMLGVLFSVPLRRSLIVDQGLTFPEGKAAAEVLKAGENPAQGVKILGGAAL
                     GGGALKLAAASGLGLVPDTAAVAGYFGRSIAYVGTNLSPALLGVGYIVGLNIGAVVFL
                     GGVLSWNVAIPLYGAFGLDGNPDLAGAVAGLSAEDAAGVLWSRQIRYLGVGAMLVGGL
                     WALFSLRTSLISGIKSGLAAARAGSQTIIAHTERDLPMKAVLLGIVLFTVPLLVLYQN
                     IVGSFAVSVPMTIIMIVAGFLFCSVSGYMAGLVGTSNNPVSGITIGTILFSAGVLLML
                     MGRGSETGPVAAIMIGAVVCCAACIAGDNLQDLKCGHIVGATPWRQQVMLAIGAFASA
                     LVLAPVLNLLARAYGIGVPTDAHPDPLLAPQATLMASVARGLFGGSLPWPTIGVGAAL
                     GAAIIAVDELLKAKRAGFRVPVLAAAVGIYLPLELSTPIFLGGLLAWLCERTLKAGGR
                     VTSPEEGERLGRRGMLFAAGVITGEALMGIVIAIPIVATGRADVLALPAPLRLGQSGL
                     GEWIGLAVLALLAYWLYRTATSVDGARPSAR"
BASE COUNT          409 a          754 c          741 g          263 t
ORIGIN      
        1 gcggctcgtg cggttctgct tcgccaagga gaacgcgacg ctcgacgccg cggcggagcg
       61 cctgcggcgg ctctgaggaa gcgacgcgag cgggtcagcg cgcgctcggg cgcgcgccgt
      121 cgacggacgt cgcggtgcga tagagccagt aggccagcag cgcaagcacc gcgagcccga
      181 tccattcgcc gaggcccgac tggcccaagc gaagcggagc gggcagcgcc agcacgtcgg
      241 cgcgtcccgt cgcgacgatc gggatcgcga tcacgattcc cattagcgct tcgcccgtga
      301 tgacgccggc cgcgaacagc attcccctgc ggccgagccg ctcgccctcc tcgggcgacg
      361 tgacgcggcc gccggccttc agcgtgcgtt cgcagagcca cgcgagcagg ccgcccaaga
      421 agatcggcgt cgaaagctcg agcggcaagt agatgcccac ggcggccgcg agcacgggca
      481 cgcggaaccc ggcacgcttc gctttgagca gctcgtcgac cgcgatgatc gcggcgccaa
      541 gagccgcgcc gacgccgatc gtcggccacg gcaacgagcc gccgaacaac ccgcgtgcca
      601 ccgacgccat caacgtcgcc tgcggcgcga gcagcggatc gggatgcgcg tcggtcggga
      661 cgccgatgcc gtaggcgcga gcgagcaagt tcaggacggg tgccaacacg agcgcgctcg
      721 cgaacgcgcc gattgcgagc atgacctgct ggcgccacgg cgtcgcgccg acgatgtggc
      781 cgcacttcaa gtcctgaaga ttgtcgccgg cgatgcatgc agcgcagcag acgaccgcgc
      841 cgatcatgat cgctgcgacg gggccggtct cggagccgcg ccccatcaac atcagcagca
      901 cgccggcgga aaacagaatc gtgccgatcg tgatgcccga caccggattg ttcgacgtgc
      961 cgacgagacc ggccatgtaa ccggacaccg agcagaacaa gaagccggcg acgatcatga
     1021 tgatcgtcat cggcacgctg accgcgaacg agcccacgat gttctgatag agcacgagca
     1081 gcggcaccgt gaacaacact atcccgagca acacggcctt catgggcaag tcgcgctcgg
     1141 tgtgcgcgat gatcgtctgc gagccggccc gcgcggcggc gaggccgctc ttgatgcccg
     1201 agatcagcga cgtgcgcagg ctgaacagcg cccaaagacc gccgacgagc atcgcgccga
     1261 cgccgagata gcgaatctgc cggctccaga ggacgccggc ggcatcttcg gcgctcaagc
     1321 ccgcgacggc gccggcgaga tcgggattgc cgtcgagccc gaacgcgcca tagagcggga
     1381 tcgcgacgtt ccaagagagc acgccgccga gaaacacgac ggcgccgatg ttgagcccga
     1441 cgatgtaacc gacgccgagc agcgccggcg aaagattcgt gccgacgtag gcaatgctcc
     1501 tgccgaagta gccggcaacg gcggcggtgt cgggaaccaa accgagcccg ctcgcggccg
     1561 cgagcttcaa cgcgccgccg ccgagtgccg cgccgccgag aatcttgacg ccttgcgcgg
     1621 ggttctcgcc cgccttcagt acctcggcgg ccgccttgcc ttccggaaac gtgagccctt
     1681 gatccacgat cagcgaccgg cgcagcggca ccgagaacaa aacgccaagc atcccgccga
     1741 gcgccgcgat cgcgacgacc cacaggaagt cgaagtcgtc ccagaaaccc aagatcacga
     1801 gcgcgggaat cgtgaagatc acgcccgacg cgagcgaggc gccggccgag ccgccggtct
     1861 gcacgatgtt gttctcgaga atcccgccgc cgccgagcac gcgcagcacg gccatcgaga
     1921 tcacggcggc gggaatcgcc gacgcgatcg tcagccccgc gaacagcccc aggtatgtgt
     1981 tcgcggccgc gagaatcatg gcgagcacga tcgcgagcac gatcgcacgc ggcgtcagtt
     2041 gcggcgggag gttgggttgc atgtcggtgt tctttgagcc ccggtgccgc attggagcca
     2101 agttcggggc cgacggcaag ctgtgcagcg tgtcgcggcc gcccgccgcg cgcagcgagt
     2161 cggctcg
//
LOCUS       DASSNN010000817         5523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1739856, whole genome shotgun sequence.
ACCESSION   DASSNN010000817 DASSNN010000000
VERSION     DASSNN010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739856"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3726)
                     /locus_tag="VFL84_15345"
     CDS             complement(<1..3726)
                     /locus_tag="VFL84_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7610056.1"
                     /translation="MADQNVAAAQGGRRPAVVDPYELRCFKFEDVLRHELALVAARRK
                     LLDTQRGDLEAAGPPVGANPADAAWQARLLGLSFSGGGIRSATFNLGVLQALAGRGVL
                     RYVDYLSTVSGGGYIGAWLTALCQRRFARTKDDAEAAGVETGSPLELSREGFKSFEEG
                     LAWSAGDTDERGPKREDRAIRFLREFSNYLTPKLGMFSGDTWALIAIYVRNALLNQLV
                     LLLALAGVLLVPRLLSLLLGRAEADTDAKFLLWMLAALVVHVFVCFNLGRNLASITAL
                     RGGEAEHAPHVIRRIGVPLLAIGISATSWLGYVGRWPSIVAAGVGAAVGLFTWLLASA
                     SARRHAGNAWSKVEKGFWTRMVWYSVPAGAAFGLLLHAVARALFADGVYSGFKLLAAP
                     PALALAILLSGVLHVGLAGKSFSAQYTEWLSRFGGFMLIGTIGWVAFNGLVFLGPLLV
                     AWLQGLWQAALSSGWILTTLVSVVFGKDVAASSGRWSRLFMAVAPYVFIAGLGVASAA
                     LLQAGLHEGAVRFGDAAAVESARSAVYSRLQAGGGDVIGVYGEWLAQTEREWSIVAAG
                     IAASIVLTWLVSLRVDVNVFSLNGMYANRLVRCYLGASVTDRRPNRFSGLSARDDLQL
                     KVEPRSIGTTHMLVHPLADGLRAGGDPQGQKPVVYPGPYHLINTAINLVAGGNLAYQE
                     RKAGAFLLSPLYCGFQLDSSSSEGPQGKNIDAFRRTSKFSSEPRQLTLGEAMATSGAA
                     ASPNMGYHSTPAFAFLMGVFNIRLGRWVGNPHPTEGNEKTWLKTGPKIALFHLLREVF
                     GLTDKASGHVYLSDGGHFENLGLYELVRRRCRYIIACDAGADPRYGFEDLGNAIRKCR
                     TDFGVEIDLDVSQIAATDALRYNGAPCAVGSIRYSEEQVGTILYIKASRRAGVPSDVM
                     HYANENAAFPHEATSDQFFSESQFESYRRLGHFLAGEILGASRARASSDLDMDLLFRD
                     LSERWYRPSLEIASNFGRLAEEVDELFERLRTSKELGFLSRQFYPEWRNLLGASVVDA
                     SVQTDDLLHVPDDEDQLRQAFYFCNSLIQLMESVYVALNLETNWRHPDLSGWLNVFNH
                     WAWSGMFRATWAVSAATYGGRFRAFCEQRLNLTLGEVEAKQVLAGKPTEAALAAAGLN
                     GHEQHQIADQYVSAGADVVVYRLELLVRHPIKKDPPLLTFGFGYAVMRGDELVMYRVQ
                     DHLRGMGLGRRGLVALVDARSGRELELGSRDVDGLLGF"
     gene            3884..>5523
                     /locus_tag="VFL84_15350"
     CDS             3884..>5523
                     /locus_tag="VFL84_15350"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7610057.1"
                     /translation="MDLDAGSSIRWLVVAAVASVATAAAWAQKPYTTWSTYGGGGHSS
                     QYSALDQIDRSNVAKLEVAWSFPAGERSFVFNPIVVDRVMYVLARDNEIVALDAGTGK
                     ELWARPHEGAVSARGINYWQNANGSDRRLLYLNAGMLTAIDARTGNIIESFGTKGRVD
                     LRDGLARDGWDVTNVRPLHTSNPGRVIGNLMVVSLPAQGAGYQSTPGDVQAYDVVTGK
                     LAWVFHSVPREGEFGYDTWPKEGHARAGGVHNWSELTVDEANGIVFVPFGTARYDFFG
                     GNRAGNNLYANSLVALDARTGKRLWHRQLVHHDLWDYDLPQAPKLLTIRDGGRTREVV
                     AQGTKHGFLFVFDRKTGEPVWPIEERRVPQSDVPGEHTSPTQPFPTKPAPFARQTFTE
                     ADINPYLPDAEKEVLRQKLRYGRNEGLFTPPSFQGSLGVPGHNGGANWASAAVDPVNG
                     ELYIVAKNLPVMLRVEGPTAEEPSARTINGPVVTPEQAAAALAAAKDAAAKGPVRYAV
                     PYDFLRSPTNGMGAMGPPWSEITAYDLNTGEVKWRKPDG"
BASE COUNT          968 a         1892 c         1872 g          791 t
ORIGIN      
        1 gaacccgagc agcccgtcga cgtcgcggct gccgagctcg agctctctgc cgctccgcgc
       61 gtcgacgagc gcgacgagcc cacgccgccc gaggcccatg ccgcgcagat ggtcctgcac
      121 gcgatacatg acgagctcgt cgccgcgcat gacggcgtag ccgaagccga acgtcaggag
      181 cggcgggtcc ttcttgatcg gatggcgcac gaggagctcg aggcgataca cgacgacgtc
      241 ggcgccggcg ctgacgtact gatcggcgat ttgatgctgc tcgtgcccgt tcaagcccgc
      301 ggccgcgagc gcggcctctg tcggctttcc cgcgagcacc tgcttcgctt cgacctcgcc
      361 gagcgtcagg ttcaaccgtt gctcgcagaa ggcgcggaag cgcccgccgt aagtcgccgc
      421 cgagacggcc caggtggcgc ggaacatccc ggaccacgcc cagtgattga acacgttgag
      481 ccagccgctc aagtcggggt gccgccagtt cgtttcgaga ttcagcgcga cgtagacgct
      541 ctccatgagc tggatcagcg aattgcagaa gtagaacgcc tgccgcagct gatcctcgtc
      601 gtcgggcacg tggagcaaat cgtccgtttg cacgctcgcg tcgacgacgc tcgcgccgag
      661 caggttgcgc cactcggggt aaaactgccg gctcaggaag ccgagctctt tcgaggtacg
      721 cagccgctcg aagagctcgt cgacttcctc ggccagccgg ccgaagttcg acgcgatctc
      781 caggctcggc cgataccagc gttcggagag atcgcggaac agcagatcca tgtcgaggtc
      841 gctcgaggcg cgagcgcggc tcgcaccgag tatctcgccg gcgaggaaat gcccgagccg
      901 ccgatagctc tcgaactgcg actccgagaa gaactgatcg gacgtcgctt cgtgcggaaa
      961 ggccgcgttc tcgttcgcgt agtgcatgac gtcggagggc acgcccgcgc gccgcgacgc
     1021 cttgatgtag agaatcgtcc cgacttgctc ctcggagtag cgaatcgacc cgaccgcgca
     1081 cggcgcgccg ttgtagcgca gcgcgtcggt agcggcgatc tgtgacacgt cgaggtcgat
     1141 ctcgacgccg aagtccgtgc ggcacttgcg gatcgcgttg ccgagatcct cgaatccgta
     1201 gcgcgggtcg gccccggcgt cgcacgcgat gatgtagcgg cagcgccggc gcacgagctc
     1261 gtacagcccc aagttctcga agtgcccgcc gtcggagaga tacacgtgac cgctcgcttt
     1321 gtcggtgagc ccgaagacct cgcgcaggag atggaacagc gcgatcttcg ggcccgtctt
     1381 cagccacgtc ttctcgttgc cctcggtcgg atgcggattg ccgacccaac gaccgagccg
     1441 gatgttgaag acccccatca ggaacgcgaa cgccggcgtg ctgtgatagc ccatgttcgg
     1501 gctcgcggcg gcacccgacg tcgccatggc ctcgccgagc gtgagctgcc gcggctccga
     1561 cgagaacttc gaggtgcgcc gaaacgcgtc gatgttcttg ccttgcggcc cttcgctgct
     1621 cgaggagtcg agctggaatc cgcagtagag cggtgacagc aagaaggcgc ccgccttgcg
     1681 ctcctgatac gcgagattgc cgccggcgac gaggttgatc gccgtgttga tgaggtgata
     1741 agggccggga tacacgaccg gcttctgacc ttgcggatcg ccgccggccc tgaggccgtc
     1801 cgccagcgga tgcacgagca tgtgggtcgt tccgatcgag cgcggctcga ccttgagctg
     1861 caaatcgtcg cgcgcggaga gccccgagaa ccggttcggc ctgcggtccg tgaccgaggc
     1921 gccgaggtaa cagcggacga gccggttcgc gtacatgccg ttcagcgaga agacgttgac
     1981 gtcgacgcgc agcgagacga gccaggtcag cacgatcgac gccgcgatcc ccgcggcgac
     2041 gatcgaccac tcgcgctcgg tctgcgcgag ccactcgccg tagacgccga tgacatcgcc
     2101 gccgcccgcc tgcagccgcg aatagacggc gctgcgcgca ctctcgacgg cggccgcgtc
     2161 gccgaagcgg acggcgccct cgtgcaggcc cgcttgcagc agcgctgcgg acgcgacgcc
     2221 gagcccggca atgaacacgt aaggcgcgac cgccatgaac agccgcgacc atcgtcccga
     2281 cgacgcggcg acgtctttgc cgaagacgac cgagaccagc gtcgtcagaa tccagcccga
     2341 ggacagcgcc gcctgccaga gcccttgcag ccacgccacg agcagcggcc ccaggaacac
     2401 gagcccgttg aacgcgaccc aaccgatcgt gccgatcagc atgaagccgc cgaaccggct
     2461 cagccactcc gtgtactgcg cgctgaagct cttgccggcc aggccgacgt gcagcacgcc
     2521 ggagagcaga atcgcgagcg cgagcgcggg cggcgccgcg aggagcttga agcctgagta
     2581 aaccccgtcg gcgaacagag cgcgcgcgac cgcgtgcagc aacaggccga aagcggcgcc
     2641 ggccgggacc gagtaccaga ccatgcgcgt ccaaaatccc ttttcgacct tgctccatgc
     2701 attgcccgcg tggcggcgcg ccgatgcgct cgccagcaac cacgtgaaga ggcccacggc
     2761 cgcaccgacg ccggccgcga cgatcgacgg ccagcggccg acgtagccga gccagctcgt
     2821 cgcggagata ccgatcgcga gcaacggcac gccgattcgc ctgatgacgt gcggcgcgtg
     2881 ctcggcctcg ccgccgcgca gcgccgtgat gctcgcgagg ttcctgccga ggttgaagca
     2941 gacgaagacg tgcacgacga gggccgcgag catccacagc aggaacttcg cgtcggtgtc
     3001 cgcttcggcg cgcccgagca gcagcgacag caggcgcggc accagcagca cgccggcgag
     3061 cgccaagagc agcacgagct ggttcaacaa cgcgttgcgc acgtagatcg cgatcaacgc
     3121 ccacgtgtcg cccgagaaca tgccgagctt cggcgtcagg tagttgctga actcgcgcag
     3181 gaagcggatc gcgcgatcct cgcgcttcgg cccgcgctcg tcggtgtcgc cggcggacca
     3241 cgccagccct tcctcgaacg acttgaaccc ttcgcgggag agctcgaggg gcgagccggt
     3301 ctcgacgccg gcggcctcgg cgtcgtcctt cgtgcgcgcg aatcgccgct gacacagcgc
     3361 cgtgagccaa gcgccgatgt agccgccgcc cgataccgtc gataggtagt cgacgtagcg
     3421 cagcacgccg cgcccggcga gggcttgcag cacgccgaga ttgaacgtgg ccgagcgaat
     3481 gccgcccccg gagaacgaca gcccgagcaa ccgcgcctgc caggccgcgt cagcggggtt
     3541 cgcgccgacc gggggcccgg cggcttcgag atcgccgcgc tgcgtgtcga gcagcttgcg
     3601 gcgggccgcg acgagcgcga gctcgtgccg cagcacgtcc tcgaacttga agcagcggag
     3661 ctcgtacggg tcgacgacag cgggacgacg gccgccttgc gcggctgcga cgttctgatc
     3721 ggccatcgaa gcacctcgtg cgcgatgtag ttgttcgttg gtgcagctgc tttatagcgc
     3781 aaaagctctc gccgggtctg tcaactgctt gacagatacg cggcgcgccg cggccgagaa
     3841 acccgggtag actttccgaa gacgatcatt ggggggctgc cgtatggacc ttgatgccgg
     3901 ctcatcgatc cgttggctcg tcgtcgccgc cgtcgcgagc gtcgcaacgg cggcggcctg
     3961 ggcgcaaaag ccgtacacga cctggagcac ctacggcggc ggcggacact cgtcgcagta
     4021 ctcggcgctc gatcagatcg acaggtcgaa cgtcgcgaag ctcgaagtcg cgtggagctt
     4081 ccccgccggc gagcgcagct tcgtgttcaa tccgatcgtc gtcgaccgcg tcatgtacgt
     4141 gctcgcgcgc gacaacgaga tcgtcgcgct cgacgcgggc acgggcaaag agctctgggc
     4201 gcggccccac gaaggcgcgg tctcggcgcg cggcatcaac tactggcaga acgcgaacgg
     4261 cagcgaccgg cgcctgctct acttgaacgc cggcatgttg acggcgatcg acgcgcgcac
     4321 cggcaacatc atcgagagct tcggcaccaa aggccgcgtc gacctgcgcg acggtctcgc
     4381 tcgcgacggc tgggacgtca cgaacgtgcg gccgctgcac acgagcaatc ccggccgcgt
     4441 catcggcaat ctcatggtcg tgtcgttgcc cgcgcagggc gcgggctatc aatcgacgcc
     4501 gggcgacgtg caggcgtacg acgtagttac cggcaagctc gcgtgggtct tccatagcgt
     4561 cccgcgcgag ggggaattcg gttacgacac gtggccgaaa gagggacacg cgagggccgg
     4621 cggcgtgcac aactggagcg agctcaccgt cgacgaagcg aacggcatcg tgttcgttcc
     4681 gttcggcacc gcgcgctacg actttttcgg cggcaatcgc gcgggcaaca acctctacgc
     4741 gaactcgctc gtcgcgctcg acgcgcgcac gggcaagcgg ctctggcatc ggcagctcgt
     4801 gcatcacgac ctctgggact acgacctgcc gcaagcgccg aagctgctga cgatccgcga
     4861 cggcgggcgc acgcgcgagg tcgtcgcgca aggcacgaag cacggtttcc tgttcgtgtt
     4921 cgaccggaag accggcgagc ccgtgtggcc gatcgaggag cgccgcgtgc cgcaatcgga
     4981 cgtgcccggc gagcacacgt cgccgacgca gccgttcccg acgaagccgg cgccgttcgc
     5041 gcgccagacc ttcaccgagg ccgacatcaa cccgtacttg ccggacgccg agaaggaagt
     5101 cttgcggcag aagctgcgct acggccgcaa cgaaggcctg ttcacgccgc cgagcttcca
     5161 gggctcgctc ggcgtgcccg gccacaacgg cggcgcgaac tgggcgagcg cggccgtcga
     5221 tcccgtgaat ggcgagctct acatcgtcgc gaagaatttg cccgtcatgc tgcgcgtcga
     5281 ggggccgacg gccgaggagc cgtcggcacg cacgatcaac ggtcccgtcg tgacgccgga
     5341 gcaggccgcc gcggcgctcg ccgctgcaaa ggatgcggcg gcgaagggtc cggttcgcta
     5401 cgccgtgccg tacgacttcc tgcgcagccc aacgaacggc atgggcgcga tgggcccgcc
     5461 gtggtcggag atcaccgcgt acgacttgaa caccggcgag gtcaaatggc gcaagcccga
     5521 tgg
//
LOCUS       DASSNN010000818         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1740476, whole genome shotgun sequence.
ACCESSION   DASSNN010000818 DASSNN010000000
VERSION     DASSNN010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740476"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VFL84_15355"
     CDS             <1..841
                     /locus_tag="VFL84_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610058.1"
                     /translation="LNGTPVQRPESFGDRRYLTDEEYAERVERLRGLNARYDDEIANN
                     KMGSGHWAEMGRPNRLTSLIVEPANGRLPPLTPEGERRSATMTSSWSSIPFDKVADFN
                     ALDRCITRGMPASMFPFMYNSGIEIMQAPGYVVIRLELVHETRVVPVDGRAPLAPEIV
                     QWLGESRGRFVGDTLVIETTNFNGETPMLIVGPGAKPIPTSAEMRIVERLTRSSEDTI
                     DYEIAVEDPVVLAEPWKAAFPLQRDDAYRMFEYACHEDNSAIRNFIETSRFERRQQAR
                     VTP"
     gene            838..1572
                     /locus_tag="VFL84_15360"
     CDS             838..1572
                     /locus_tag="VFL84_15360"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7610059.1"
                     /translation="MTKPADEYQRLLALIEDQKQRLKSLGAGREESMRALVDLRDELK
                     RVSRERDELRKQLARLDRMQTETIALPEDFRFPSAGSVPSLDELMSELGDIEEPGSGR
                     VEGHLHQRVESAAQSEEMISPEVVFPEKFAATAESAEQKAPVSRVLVLLDAERPIKYP
                     LFKETMTIGRAESADIQINNDFLSRLHARIVSTPDGAAIEDIESKNGIRVNTKLVTKR
                     QGLRHGDIVDLGRLRFRYIDAAYDAD"
     gene            complement(1595..>2099)
                     /locus_tag="VFL84_15365"
     CDS             complement(1595..>2099)
                     /locus_tag="VFL84_15365"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET7610060.1"
                     /translation="IYFTVGGAYYMNPSGVVSTVADQDIRSNGIMLSPDGRTLYVTNN
                     TSVLAFDVRGDGTTANRRDFGTLDGDDGGDGMAVDSEGRLYVTGNRGVHVLSPRGEHL
                     GLIPTPRRPIAVAFSGADKKLLYAPMSGAVGPDGKAWATPETIRNTAMTIYRLTMLSQ
                     GYKGRPK"
BASE COUNT          383 a          699 c          691 g          326 t
ORIGIN      
        1 gctgaacggc acgccggtcc agcgacccga gagcttcggt gaccggcgct acctcaccga
       61 cgaagagtac gccgagcgag tcgagcgcct gcgaggcctg aacgcgcgct acgacgacga
      121 gattgccaac aataaaatgg gcagcggcca ctgggccgag atgggtcggc cgaacagact
      181 gacgtcgctg atcgtggagc ccgcgaacgg ccgtctgccg ccgttgacgc cggaaggcga
      241 gcgccgctcg gccaccatga cgagcagctg gtcgagcatc ccgttcgaca aggtcgccga
      301 tttcaacgcg ctcgaccgct gcatcacgcg cggcatgccg gcctcgatgt ttccgttcat
      361 gtacaacagc ggcatcgaga tcatgcaggc gcccgggtac gtcgtgatac gcctcgagct
      421 cgtgcacgag acgcgcgtcg tgcccgtcga cggccgcgcg ccgctcgcgc ccgagatcgt
      481 gcaatggctc ggcgaatcgc gcggccggtt cgtgggcgac acgctcgtca tcgagaccac
      541 gaacttcaac ggcgagacgc cgatgctgat cgtcggcccc ggcgccaagc cgattccgac
      601 gagcgccgag atgcgcatcg tcgagcggct cacgcgctca agcgaagaca cgatcgatta
      661 cgagatcgcc gtcgaggacc ccgtcgtgct cgccgagccg tggaaagccg cgtttccgct
      721 gcagcgcgac gatgcgtatc gaatgttcga gtacgcctgc cacgaggaca attcggccat
      781 tcgcaatttc atcgagacgt cgcgcttcga gcggcgccag caagcgcgag taacaccatg
      841 acgaagccag ccgacgagta ccagcgtctt ctcgcgctga tcgaggatca aaagcagcgg
      901 ctgaagtcgc tcggcgcggg acgcgaggaa tcgatgcgcg cgctcgtgga tctgcgcgac
      961 gagctcaagc gcgtgagccg agagcgcgac gagctccgca agcagctggc aaggctcgac
     1021 cgcatgcaga ccgagacgat cgctctgccc gaagacttcc gtttcccgtc cgcagggtcg
     1081 gtgccgtcgc tcgacgagct catgagcgag ctcggcgaca tcgaggagcc cggcagtggc
     1141 cgcgtcgaag gtcacttgca ccagcgcgtc gagagcgcgg ctcagtccga ggagatgatc
     1201 tcgccagagg tcgtctttcc cgagaagttc gccgcgacgg ccgagtcggc ggagcagaaa
     1261 gcgcccgtct cgagggtact cgtgctgctc gacgccgagc ggcccatcaa gtacccgctc
     1321 ttcaaggaga cgatgacgat cggccgcgcc gagagcgccg acatccaaat caacaacgac
     1381 ttcctgagcc ggctgcacgc gcgcatcgtc tcgacgccgg atggtgccgc gatagaggac
     1441 atcgagagca agaacggcat ccgcgtcaat acgaagctcg tgacgaagcg ccaaggcctg
     1501 cggcacggcg acatcgtaga cttgggccgg ctgcgcttcc gttacatcga cgccgcctac
     1561 gacgcggatt gacggcgctt acgccgttcg ctccctactt cggccggcct ttgtatccct
     1621 gcgacagcat cgtcaggcga tagatcgtca tcgccgtgtt gcggatcgtc tccggcgttg
     1681 cccacgcttt gccgtcggga ccgacggcgc cgctcatcgg cgcgtagagc agcttcttgt
     1741 ccgcgcccga gaacgcgacg gcgatcggcc gccgcggcgt cgggatcaac cccaagtgct
     1801 cgccgcgcgg cgacagcacg tgcacgccgc gattgccggt gacgtagagg cgcccttccg
     1861 aatcgacggc catgccgtcg ccgccgtcgt cgccgtcgag cgtgccgaaa tcgcgccggt
     1921 tcgccgtcgt gccgtcgccg cgcacgtcga acgcgagcac gctcgtgttg ttcgtcacgt
     1981 acagcgtgcg gccgtcgggg gagagcatga tgccgttcga gcggatgtct tgatccgcga
     2041 cggtgctgac gacgccgctc ggattcatgt agtaggcgcc gccgacggtg aagtagatt
//
LOCUS       DASSNN010000819         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1361189, whole genome shotgun sequence.
ACCESSION   DASSNN010000819 DASSNN010000000
VERSION     DASSNN010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361189"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..458
                     /locus_tag="VFL84_15370"
     CDS             252..458
                     /locus_tag="VFL84_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610061.1"
                     /translation="MGDKNQGEGNRDADRRYRRGVSETVRKTTEEERADRARDLTPEE
                     QEAARQAEEQGKAKKRPASSEREI"
     gene            455..700
                     /locus_tag="VFL84_15375"
     CDS             455..700
                     /locus_tag="VFL84_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610062.1"
                     /translation="MSAARCERCGNEYDQSFTVLLANGDRRIFDCFECAIGALAPKCA
                     RCGVTIIGHGVQAYGAIYCCAHCARGEGVDRVRDRVS"
     gene            complement(716..>2489)
                     /locus_tag="VFL84_15380"
     CDS             complement(716..>2489)
                     /locus_tag="VFL84_15380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7610063.1"
                     /translation="EPAMRSKTKRTTHETVGAAADADAIRMRDPVGSGAVPSADEAPR
                     RAGSDAEARLEAEIALLRSCAEVLLASDGVGAAAEEVFVKLAPSFELDAFAAFVATGA
                     DHALTLEAVAGLTDTAISSTAIAGIVAELESAGRRTLVLDSLSRNPDPKARIFRDLGF
                     ASAVVGALVVDARMLGAVVLASRSKERFTADELMLVDTVVCYLAAASERRELARHRNT
                     AEHRKEEFLNVLAHELRNPLAPVRNALEIIRANDRTHSAVEHSAYAMIERQLQQIGRL
                     ADDLLDANQVVLGHVELAKQRVLLDAVVERAAEQSRPWIEAARHSLSVELPREAIALD
                     ADPKRLARALSNLINNAARRLDRGGRIVVSAESAEAAVTVFVRARGRALSSAALERVF
                     AEDEQSGNGHGETQGGVTAGMTLVKHLVAMHGGAVAAQTDADGLVEFAVRLPLVQARV
                     SAARTAARPVAGEPTEPLAPHRILIADDNADSAESMGMLLRLMGNDVRIASDGLEAVE
                     QAETFQPDIVLMDIGMPRLDGYEAARRIREQDWSRDTLLVAVTGWGPTDDSDDATAAG
                     FDCHFTKPLDPAELRKLVSGVRPH"
BASE COUNT          397 a          871 c          841 g          380 t
ORIGIN      
        1 cattgccgaa cagcccggcg gccgccgcga tcagcacggc tcccatcgcg atccaaccga
       61 cccgctcgaa cgcccagctg cggcgaaagc agcgcgtgcg ctcgaaacgt gacggagtgg
      121 acgccatgcg gcttgaaggt cgtcggcgca catgcgccgc ttggttttgc aagacacatg
      181 ccacgattcg gcgcccgctg ctggcaccct ccttgcatcg cggcaggcgt tcgtcgacat
      241 ggagaggacc aatgggcgac aagaaccaag gcgagggcaa tcgcgacgcc gaccggcgct
      301 accggcgcgg cgtgagcgag acagtgcgca agacgaccga ggaagaacgc gccgatcgtg
      361 cgcgcgacct gactcccgag gagcaagagg cggcgcggca agcggaggag caaggcaagg
      421 cgaaaaagcg tcccgcctcg tcggagcgag aaatttgagc gcagcgagat gcgagcgctg
      481 cggcaacgag tacgaccagt cgttcacggt cctgctcgcg aacggcgatc gccgcatttt
      541 cgattgcttc gagtgcgcga tcggcgcgct ggcgccgaag tgcgctcgtt gcggcgtcac
      601 gatcatcggc cacggcgtgc aggcgtacgg cgcgatctat tgctgcgcgc actgcgcgcg
      661 cggcgaaggc gtcgatcgcg ttcgcgaccg cgtgtcttga cggcgcgcgc gccctctagt
      721 gcgggcgcac tccactgacg agcttgcgca actccgccgg gtcgagcggc ttcgtgaagt
      781 ggcagtcgaa gcccgcggcc gtcgcgtcgt cgctgtcgtc ggtcggtccc caacccgtga
      841 ccgccacgag cagcgtgtcg cggctccaat cctgctcgcg gatcctgcgc gcggcttcgt
      901 aaccgtcgag ccgcggcatg ccgatgtcca tcagcacgat atccggttgg aacgtctccg
      961 cctgctcgac ggcctcgagt ccatcactcg ctatgcgcac gtcgtttccc atgagccgca
     1021 gcagcatccc catgctctcg gccgaatcgg cgttgtcgtc ggcgatcaat attcggtgcg
     1081 gcgccagcgg ctccgtgggc tctcccgcga ccggccgagc ggccgtgcgc gccgccgaaa
     1141 cgcgcgcctg gacgagcggc aggcgcaccg cgaactcaac cagcccgtcg gcgtcggtct
     1201 gcgccgcaac ggctccgccg tgcatcgcga cgagatgctt cacgagcgtc atcccggccg
     1261 tgacgccacc ctgcgtctcg ccgtgaccgt tgcccgactg ctcgtcctcg gcgaatacgc
     1321 gctcgagcgc cgcgctcgac aatgcgcggc cgcgcgcgcg gacgaacacg gtaaccgccg
     1381 cttcggccga ctccgccgag acgacgattc gtccgccgcg gtccaagcgc cgcgcagcgt
     1441 tgttgatcag gttcgacaaa gcccgcgcca agcgcttggg atcggcgtcg agcgcgatcg
     1501 cctcgcgcgg caactcgacc gagaggctgt ggcgcgccgc ctcgatccag gggcgcgatt
     1561 gctccgcggc gcgctcgacg acggcgtcga gcagcacgcg ctgcttcgcg agctcgacgt
     1621 ggccgagaac tacttgattc gcgtcgagaa gatcgtcggc aagcctgccg atctgttgca
     1681 gctgacgctc gatcatcgcg taagccgaat gctcgaccgc cgaatgcgtg cgatcgttgg
     1741 cgcgaatgat ttcgagcgcg ttacgtaccg gcgcgagcgg attgcgcagc tcgtgcgcga
     1801 gcacgttcag gaattcttcc ttgcggtgct ccgcggtgtt gcggtgccgc gcgagctcgc
     1861 ggcgctccga cgcggcggcc aggtagcaga cgacggtgtc tacgagcatc agctcgtcgg
     1921 cggtgaagcg ttccttcgag cgcgacgcca gcacgacggc tcccagcatg cgcgcgtcga
     1981 cgacgagcgc gccgactacc gcgctcgcga aaccgagatc gcgaaatatc cgcgctttcg
     2041 ggtccgggtt gcgcgacagc gagtcgagca ccagtgtgcg ccggcccgcg ctctcgagct
     2101 cggcgacgat acctgcaatg gccgtgctcg agatcgcggt gtccgtcaac cccgccaccg
     2161 cctcgagcgt cagcgcgtga tcggcgcctg tcgcgacgaa tgcggcgaaa gcgtcgagct
     2221 cgaacgacgg cgcaagcttg acgaaaactt cctccgcggc cgcgccgacg ccatcgctcg
     2281 ccaacagcac ttcggcgcag ctgcgcagca gcgcgatctc cgcctcgagg cgagcttcgg
     2341 cgtccgagcc cgcgcgacga ggcgcttcgt cggccgatgg caccgcaccc gagccgacgg
     2401 ggtcgcgcat tcgaattgcg tcggcgtcag cggcggcgcc gacggtctcg tgcgtcgtcc
     2461 gcttggtctt ggatcgcatc gccggctcg
//
LOCUS       DASSNN010000820         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1742926, whole genome shotgun sequence.
ACCESSION   DASSNN010000820 DASSNN010000000
VERSION     DASSNN010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742926"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VFL84_15385"
     CDS             complement(<1..838)
                     /locus_tag="VFL84_15385"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7610064.1"
                     /translation="MSARSTRAATVLSPLLVVGCIANAQTLTGEWPAYGGDLGHTRYA
                     PLDQINASNFGSLEIAWRFSVANMGPTPEMRLQSTPLVIDGVLYTTGGMRRAVTALDA
                     ATGEQLWVFGLNEGARGDQAPRQGSGRGLAFWQRGDDKRVIYVTPGYQLVALNADTGR
                     PVESFGENGIVDLKASLDQGGGWDRNQIGTNSPPTIAGDVIMVPAAHTPLAPPNQAKN
                     VVGYIRGFDAVTGRLLWTFHTVPRRGEPGYETWLNGSAETGGGNAGVWATISADPELG
                     LAY"
     gene            922..1401
                     /locus_tag="VFL84_15390"
     CDS             922..1401
                     /locus_tag="VFL84_15390"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7610065.1"
                     /translation="MRTAALFLGGLIVGAVLVQASDAQDRARRGLNHIGIGTKNYDAA
                     LAFYRDTLGAKEAFTIKNPDGTVRITYLQVSRDTFIELLPVADDQPAGILHVGIETDD
                     LAASVASLRARGITVAEPTVGVSKARVARITDPDGVNIEVMQMGPDSMQRQAIDAWR"
     gene            complement(1415..2254)
                     /locus_tag="VFL84_15395"
     CDS             complement(1415..2254)
                     /locus_tag="VFL84_15395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7610066.1"
                     /translation="MRARRMLGFLAAAALLVLLGHSASAATVDGLKLQSSSVGTGPTI
                     VFVHGWTCDSSSWTSQVPAFSKDHRVITLDLPGHGQSASPQDGKLSMDLFARAVEAVR
                     AEAGADRIVLVGHSMGAPVIRQYAHLYPQHVAGLVAVDGPLDIRVFPAELPPGFAASF
                     TGPEGRAAREGMIKSMFIAETPAALQDHILKMMLAAPEATAVGAMNATFDPANRWTDV
                     IQAPALTVYAGTANVPDPVATKELYPNHEATQLKGTGHFLMMEKPAEFNRLLAGFLDK
                     IDF"
BASE COUNT          419 a          839 c          812 g          387 t
ORIGIN      
        1 gatacgcgag cccgagctcg gggtcagccg agatcgtggc ccacacgccg gcgttgccgc
       61 cgcccgtttc ggcagagccg ttcaaccacg tctcgtagcc gggctcgcca cgccgcggca
      121 ccgtgtggaa cgtccacaag aggcggcccg tgacggcgtc gaagccgcgg atgtagccga
      181 cgacgttctt cgcctgattc ggcggcgcga gcggtgtgtg tgccgccggc accatgatga
      241 cgtcgcccgc gatcgtcggc ggcgagttcg tgccgatctg attccggtcc cagccgccgc
      301 cctgatcgag cgacgccttc agatcgacga tgccgttctc gccgaagctc tcgacgggcc
      361 ggcccgtgtc ggcgttcaac gcaacgagct gatagccggg cgtcacgtaa atgacgcgct
      421 tgtcgtcgcc gcgctgccag aatgcgagcc cgcgcccgga cccctgccgt ggcgcctggt
      481 cgccgcgcgc gccctcgttc aagccaaaca cccagagttg ctcgcccgtc gcggcgtcga
      541 gcgccgtgac ggcgcgccgc atgccgcccg tcgtatagag aacgccgtcg atgacgagcg
      601 gcgtgctctg caagcgcatc tcgggcgtcg gccccatgtt ggcgacgctg aaccgccacg
      661 cgatttcgag cgagccgaag ttgctcgcat tgatctgatc gagcggcgcg tagcgtgtgt
      721 ggccgagatc gccgccgtaa gccggccatt cgccggtcag cgtctgcgcg ttcgcgatgc
      781 agcccaccac gagcagcggc gacagaacag tggcggcgcg cgtcgagcgt gcactcatgt
      841 ttatccccct cgaatcgcgc tcatactagg ggcctgcgcg cgacggggtc aacgcgcgcc
      901 caacaacgaa ggagaattcc gatgagaact gcagcgttgt ttctcggcgg actgatcgtg
      961 ggcgcagtgc tcgtgcaagc gagcgacgcg caagaccgcg cgcggcgcgg actcaatcac
     1021 attgggatcg gaacgaagaa ttacgacgcc gcgctcgcgt tctaccgcga cacgctcggc
     1081 gcgaaagaag cgttcaccat caagaatccc gacggcacgg tgcgcatcac gtatctgcaa
     1141 gtctcgcgcg acacgttcat cgagctctta cccgtagcgg acgatcagcc cgcgggcatc
     1201 ctgcacgtcg gcatcgagac ggacgatctc gccgcgagcg tcgcgagctt gcgcgcacgc
     1261 ggcatcaccg tcgcggagcc gaccgttggg gtctcgaagg cgcgcgtcgc gcgcatcacc
     1321 gaccccgacg gcgtcaacat cgaggtcatg cagatggggc ccgactcgat gcagcgccag
     1381 gcgatcgacg cttggcgcta gacggcgagc gcgctcaaaa atcgatcttg tcgaggaacc
     1441 cggcaagcag gcggttgaac tcggcgggct tctccatcat gaggaagtga cccgtccctt
     1501 tcagctgcgt cgcttcatga ttcggataga gctctttcgt cgcgacgggg tccggaacgt
     1561 tcgccgtgcc cgcgtagacc gtcaaagccg gtgcttgaat gacgtccgtc cagcggttcg
     1621 ctggatcgaa cgtcgcgttc attgcgccga cggccgtcgc ttcgggggcg gccagcatca
     1681 ttttcaagat gtggtcttgc agcgcggccg gcgtctcggc gatgaacatc gacttgatca
     1741 tgccttcgcg tgccgcgcgg ccttcgggtc ccgtgaacga cgccgcaaag ccaggcggca
     1801 gctccgcagg aaatacgcgt atgtcgagcg gcccgtcgac ggcaacgaga cccgcaacgt
     1861 gctgcggata gaggtgcgcg tactgccgga tcaccggcgc tcccatgctg tggccgacga
     1921 gcacgatgcg gtcggcgccc gcttccgcac gtacggcctc gacggcgcgc gcgaacaggt
     1981 ccatcgacag cttgccgtct tgcggcgacg cgctctgacc gtggcccggc aagtcgagcg
     2041 tgatgacgcg gtggtccttc gagaacgcgg gcacctgcga cgtccaggac gagctgtcgc
     2101 acgtccagcc gtgcacgaac acgatcgtcg gtcccgttcc caccgatgac gactggagct
     2161 tgagtccatc gaccgtcgcg gccgaagccg agtggccgag cagcacgagc agagctgccg
     2221 ccgccaagaa gccgagcatc cttcgtgcac gcattgttca ttcccccggg tgagctcgcc
     2281 tagcctacac ccaaccgtca ctcgtggcgc agcgcctcca tcggggcgac gttcgaggcg
     2341 cgcagcgcgg gcagccagct cgcgacggcc acgacgagcg cgagcccgcc gacggccgaa
     2401 acgaatacga gcgggtcgta gcccgacaag ccgtgcaggc tcgcctgagc gagccgg
//
LOCUS       DASSNN010000821         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1746252, whole genome shotgun sequence.
ACCESSION   DASSNN010000821 DASSNN010000000
VERSION     DASSNN010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746252"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1046
                     /locus_tag="VFL84_15400"
     CDS             54..1046
                     /locus_tag="VFL84_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017322324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET7610067.1"
                     /translation="MKIFLVSKGSTGLDGLKRAERERPTPQAGQVLVRMRAASLNFRD
                     LAIVAGKYFRGPVERDTIPLSDGAGEVEGVGPGVTQFKAGERVVATFTQGHPPAALGL
                     PLDGTLAEYCVFGVDGLLKVPPHLSYEEAATLPCAGVTVWNALMHGPRVLKAGETVLS
                     LGTGGVAIFALQIAKMAGARSIITSSSDAKLERARALGADDVINYKTTPDWDKKVLEL
                     TDGQGAEHVIDTGGVGTLPKSYVAVGPGGVVSIIGVMTRPEGDLSPYPLMFKGAMARG
                     IFVGGREHFEALMKAVAVNEFKPVVDKTFDFDAAPEAFKYLKSAQHFGKVVIKI"
     gene            1208..>2904
                     /locus_tag="VFL84_15405"
     CDS             1208..>2904
                     /locus_tag="VFL84_15405"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7610068.1"
                     /translation="MNESTARDIAGYMGQLSAAVLACISLPSVAEAQSGTAEPTEEII
                     VTSSIIAQPRRQIGTAVSGIDFEEIELRGYTDLSDVLRTQTGIGVSNTGGQGKSTSVR
                     IRGEETYRTTLMIDGVKALDPSAPQVSPDFDSLLTTSDLQRVEVLRGPQGFMYGADAG
                     GVVNVITKRGADELGGRVGVEAGDYSTRKLDAAVSGGSDGGDYFLSVVDFETDGFNSR
                     TDDTVLRDDDGAENTTLHAKLGLNVADDVRLQLVARDIDAESQYDNCGFPTVHDCVGT
                     TKQTTYKLSADVSGDLFSNSFGYSDIDIAHDNFTAGVSAFATEGALSRFEYTGSYKPS
                     DALALVYGVDLQEEELTDTDGTLSRDQDGYYVEYQGAFDDAFFLALGARYDDNEDFGT
                     HTSSRLSVAYVQDLSSERSLKYRASAGTGFRAPSMYELAYNRGPFAFPPASLNPNLTE
                     ETSRGYDVGIEYNAANGLHFEATYFDQEIEDAIEFDLVGFSGYLQTQGTSTSSGVEVA
                     ADIPVGERWQLLANWTYNDTEDSAGVQRVRRPKNLGNLGVAYRTDDERLRLLANYRIS
                     "
BASE COUNT          525 a          953 c          954 g          472 t
ORIGIN      
        1 agatccgtcc cctttttgcg gctacagtcg agggctcgaa gcggagcagt cgcatgaaga
       61 ttttcctcgt ctccaaaggc tctacggggt tagacgggct caagcgcgcc gagcgcgagc
      121 ggcccacgcc gcaagccggc caggtgctcg tgcgcatgcg cgccgcgtcg ctgaatttcc
      181 gcgatctcgc gatcgtcgcc ggcaagtatt ttcgggggcc ggtcgagcgg gacacgattc
      241 cgctgtcgga tggcgccggc gaggtcgagg gagtcggacc cggagtcacg caattcaaag
      301 ccggcgaacg tgtcgtcgcg acgttcacgc aaggtcatcc gccggccgcg ctcggcctgc
      361 cgctcgacgg cacgctcgcc gaatactgcg tgttcggagt cgacgggttg ctgaaggtgc
      421 cgccgcacct gtcgtacgag gaagccgcga cgctaccgtg cgccggcgtg acggtctgga
      481 acgcgctgat gcacggcccg cgcgtcttga aggccggcga gacggtgctt tcgctcggca
      541 cgggcggcgt cgcgatcttc gcgctgcaga tcgcgaagat ggccggcgcg cgctcgatca
      601 tcacgtcgtc gagcgacgcg aagctcgagc gtgcacgcgc gctcggcgcc gatgacgtca
      661 tcaactacaa gacgacgccg gattgggaca agaaggtcct cgagctcacg gacggccagg
      721 gcgcggagca cgtcatcgat acgggcggcg tcggcacgct gccgaaatcc tacgtcgctg
      781 tcggcccggg cggcgtcgtg agcatcatcg gcgtcatgac gcgccccgag ggcgacctga
      841 gcccctatcc gctgatgttc aagggcgcga tggcgcgcgg catcttcgtc ggcggccgcg
      901 agcatttcga ggcgctcatg aaggccgtcg ccgtcaacga attcaagccc gtcgtggaca
      961 agaccttcga cttcgacgcg gcgccggagg ctttcaagta cctgaaatcc gcgcagcact
     1021 tcggcaaggt cgtcatcaag atctagggcc gcagctgctt tttcgccggc taacggttaa
     1081 ttgaacggtt attaacgcgg tccggcgccc ggattcggct aggctgcgtc ctcgcgttta
     1141 ggggcgtcgc gccgcaattc gcgcgcccga caaaaactac gatcaagcgc atcgatcgag
     1201 ggagtctatg aacgagtcca cggcaaggga tatcgcgggc tatatgggtc agctgtccgc
     1261 ggccgtattg gcctgtatct cactgccgtc cgtggcggaa gcgcagagcg gcacggccga
     1321 gccgaccgaa gagatcatcg tcacgtcgag catcatcgcg cagccgcgcc ggcagatcgg
     1381 cacggccgtt tccggcatcg acttcgagga gatcgagctg cgtggctaca ccgacctgtc
     1441 cgacgtgctg cgcacgcaga ccgggatcgg cgtcagcaac acgggcgggc aaggcaagtc
     1501 gacctcggtg cgcattcgcg gcgaggagac ctaccgcacg acgctgatga tcgacggcgt
     1561 caaagcgctc gacccgagcg cgccgcaagt ttctccggac ttcgacagcc tgctgacgac
     1621 gagcgatctg cagcgcgtcg aggtgctgcg cggcccgcag ggcttcatgt acggcgcgga
     1681 cgccggcggc gtcgtcaacg tcatcacgaa gcgcggcgcc gacgagctcg gcgggcgtgt
     1741 cggcgtcgag gccggcgact actcgacgcg caagctcgac gccgcggttt cgggcggcag
     1801 cgatggcggc gactacttcc tttcggtcgt cgatttcgag accgacggtt tcaactcgcg
     1861 caccgacgac acggtgctgc gcgacgacga cggcgccgag aacacgacgc tgcacgcgaa
     1921 gctcggcttg aacgtcgcgg acgacgtgcg cctgcagctc gtcgcgcgcg acatcgacgc
     1981 cgagtcgcaa tacgacaact gcggcttccc gaccgtgcac gactgcgtcg gcacgaccaa
     2041 gcagacgacg tacaagctct ccgccgacgt ctccggcgac ttgttctcga actcgttcgg
     2101 ctacagcgac atcgacatcg cgcacgacaa cttcaccgcg ggcgtttccg ccttcgccac
     2161 ggaaggcgcg ctttcgcgct tcgagtacac gggcagctac aagccatccg acgcgttggc
     2221 gctcgtctac ggcgtcgatc tgcaagaaga ggagctgacc gacaccgacg gcacgctgag
     2281 ccgcgaccaa gacggctact acgtcgaata tcaaggcgcc ttcgacgatg cgttcttcct
     2341 ggcgctcggc gcgcggtacg acgacaacga agattttggc acgcacacga gctcgcgctt
     2401 gagcgtcgcc tacgtgcaag acctgagctc ggagcgttcg ctgaagtatc gcgccagcgc
     2461 cggcaccggc ttccgcgcgc cgagcatgta cgagctcgct tacaaccgcg gcccgttcgc
     2521 gttccccccg gcgtcgctga atccgaacct gacggaggaa acgagccgcg gctacgacgt
     2581 cggcatcgag tacaacgccg ccaacgggct gcatttcgaa gcgacgtact tcgaccagga
     2641 gatcgaagac gcgatcgagt tcgatctcgt gggtttctcc ggctatttgc aaacgcaagg
     2701 cacgagcacg tcgagcggcg tcgaggtcgc ggccgatatt ccggtcggcg agcgctggca
     2761 gctgctcgcc aactggacgt acaacgacac cgaagattcc gccggtgtgc agcgcgtccg
     2821 ccgtcctaag aatctcggca acttgggcgt cgcgtaccga acggacgacg agcggctcag
     2881 gctgctcgcg aactaccgca tctc
//
LOCUS       DASSNN010000822         4002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_732161, whole genome shotgun sequence.
ACCESSION   DASSNN010000822 DASSNN010000000
VERSION     DASSNN010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732161"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2567)
                     /locus_tag="VFL84_15410"
     CDS             complement(<1..2567)
                     /locus_tag="VFL84_15410"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HET7610069.1"
                     /translation="MTTPRRWLISLVLLFAALPASAQDRVRVTYLFSDGQMPVTLAAY
                     KELLEQHPELRDRVELGFVTESVFDDVDAAKAERSDVLVLDVMNQQMVDRFNTEHDTN
                     LIERINERGSVIGVGEGLLPKESYLAQGVTWDDRARALWANSGLENQLGLLKYALSAA
                     GVPGLDVPDPTPALDFGYYYPGAPGLFATWREVDAWRRANGKAKPGAPRIAVGFYKST
                     YYTDDTATLDAVIAEIEKAGGEAIPVFGYPGGVTFERLLLDENGAARADVGLAFLFRF
                     TGPEAAESLSKLDFPVINLVTLYGRSAKEWRESRTGLSMFEGTFQVAVPELGGLVAPT
                     VVGSREKILDLETGLSVVTNRPIPSRVAMAVQRALRYAALRAKPNAEKHIALMYYNYP
                     PGKANIGASYLNVAESLANILARLRAEGYDVGSGEITGDALLAELTAKAYNVGSYAPG
                     ELEELVAAGGAIRVPVAQYREWLAALSPALRDKILADWGEPEKSELMVAGGGASASIV
                     VPLVRRGNVVLLPQPPRGWGEDADKLYHAKDLAPHHQYLAAYAWLRNAFRADAVVHLG
                     THGTLEWLDGRDVGQNEEDASDALIADLPDIYVYNVDVVGEGLIARRRGMATLVDHMV
                     PPFVGGGLYPELAELGERINDYDTNLHKNPELATAFAGQVRDLVLSLGIAKDLGLELP
                     DAASLTDPIVHQIQDYLVELKSQNIPYGLHAFGRVPDDAARASTVGAIVSADRSLLPN
                     DANVLAADIDARIVASGPRELDNLVRALSDGFVPVGAGGEPIRNPDSYPTGKNFYGID
                     PEKVPKRASWELGVKLADEMLAKHLEEHGRYPEKVSFVIWGDETMRHEGVLESQIFWL
                     "
     gene            complement(2564..2911)
                     /locus_tag="VFL84_15415"
     CDS             complement(2564..2911)
                     /locus_tag="VFL84_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HET7610070.1"
                     /translation="MSKFLKLADPDEQDEDPLAGVANLFDVSVVFIVGLMISLFSIYR
                     MRDLIDPDSEVTMVKTNAQGESEVIIKKGTEIEAYKVTGEQLAGDGERLGTAYRLANG
                     QIIYVPDNEAPPP"
     gene            complement(2914..3537)
                     /locus_tag="VFL84_15420"
     CDS             complement(2914..3537)
                     /locus_tag="VFL84_15420"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7610071.1"
                     /translation="MNLLGLLENGLFALGQVLRFPVMALLWICVLAVLFMAGSAVVEA
                     LARRRERNGFDVEAWLKQGRVLGADAARRAQLPAAMKRLLADVERGRAEARLANGGLE
                     HLVLASEERTRKSLSGSRLLVKVGPSLGLLGTLIPMGTALAALSTGNLDAMAGQMVVA
                     FTTTIIGLACGTAAYVILTIRQHWVTEAVREQRFLAERIAAELAQGA"
     gene            complement(3534..>4002)
                     /locus_tag="VFL84_15425"
     CDS             complement(3534..>4002)
                     /locus_tag="VFL84_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610072.1"
                     /translation="AAFVFARLGALDVTALGFCLAAAAVAALARPQWRLLPPLAAGIS
                     AAAWISILQAQGLPWLPAAAVAVFVTAAAVVLAALRRGFVTADLRDEALVIVAAFALL
                     LGLGPGVVEGWRSSVALQSEPLASAGPQIEPWLGALVIVTALLGGAYTIWKRR"
BASE COUNT          635 a         1385 c         1363 g          619 t
ORIGIN      
        1 agccagaaga tctgcgactc gagcacgccc tcgtggcgca tcgtctcgtc gccccagatc
       61 acgaacgaga ctttctcggg ataacggccg tgctcctcga ggtgcttcgc gagcatctcg
      121 tcggcgagct tgacgccgag ctcccacgac gcgcgcttcg gcaccttctc gggatcgatg
      181 ccgtagaagt tcttgccggt cgggtacgaa tcgggattgc ggatcggctc gccgccggcg
      241 ccaacgggca cgaagccgtc gctcaaggcc cgcacgaggt tgtcgagctc gcgcggcccc
      301 gacgcgacga tgcgcgcatc gatgtcggcg gcgagcacgt tcgcatcgtt cggcagcaag
      361 cttcggtcgg cgctgacgat cgcgccgacg gtgctcgcgc gcgccgcgtc gtcgggcacg
      421 cggccgaacg cgtgcaggcc gtacgggatg ttctggctct tgagctcgac caaatagtct
      481 tggatttggt ggacgatggg gtcggtcagg ctcgcggcat cgggcagctc gagccccaag
      541 tcttttgcga tgccgagcga cagcacgaga tcgcggactt ggcccgcgaa cgccgtcgcg
      601 agctcgggat tcttgtgcag attcgtgtcg tagtcgttga tgcgctcgcc gagctcggcg
      661 agctcggggt agaggccgcc gccgacgaac ggcggcacca tgtgatcgac gagcgtcgcc
      721 atgccgcggc ggcgcgcgat caagccctcg ccgacgacgt cgacgttgta gacgtagatg
      781 tccggcaggt ccgcgatcag cgcgtcggac gcgtcctcct cgttctgccc gacgtcgcgg
      841 ccgtcgagcc attcgagcgt gccgtgcgtg cccaaatgca cgacggcgtc ggcgcgaaac
      901 gcgttgcgca gccatgcgta cgcggcgaga tattgatggt gcggcgcgag atctttcgcg
      961 tgatagagct tgtcggcgtc ttcgccccaa ccgcgcggcg gctggggcag cagcacgacg
     1021 ttgccgcgcc gcacgagcgg cacgacgatg ctcgcgctcg cgccgccgcc ggccaccatg
     1081 agctcggatt tctcgggctc gccccagtcc gcgagaatct tgtcgcgcag agccggcgag
     1141 agcgcggcga gccactcgcg gtattgcgcg acgggaacgc ggatcgcgcc gcccgccgcg
     1201 acgagctcct cgagctcgcc gggcgcgtag ctgccgacgt tgtacgcctt cgccgtcagc
     1261 tcggcgagca aggcatcgcc cgtgatctcg ccggagccga cgtcgtagcc ctcggccctc
     1321 agccgcgcca ggatgttcgc gagcgactcc gcgacgttca gatagctcgc gccgatgttg
     1381 gctttgcccg gcggatagtt gtagtacatc agcgcgatgt gcttttcggc gttcggcttc
     1441 gcgcgcagcg ccgcatagcg cagcgcgcgc tgcacggcca tcgccacgcg cgacggaatg
     1501 ggcctgttcg tgacaaccga gagcccggtc tcgaggtcga ggatcttctc gcggctgccg
     1561 acgacggtcg gcgccacgag gccgccgagc tcgggcaccg cgacctggaa cgtgccctcg
     1621 aacatcgaga ggccggtgcg cgactcgcgc cattctttcg cgctgcggcc gtacagcgtc
     1681 acgagattga tgacggggaa gtcgagcttc gacagcgact cggccgcctc cggtcccgtg
     1741 aagcggaaca ggaacgcgag acccacgtcg gcgcgcgcgg cgccgttctc gtcgagcagc
     1801 aaccgctcga acgtgacgcc gcccgggtag ccgaacaccg ggatcgcctc gccgccggct
     1861 ttctcgatct cggcgatcac ggcgtcgagc gtggcggtat cgtccgtgta gtacgtcgac
     1921 ttgtagaacc cgaccgcgat gcgcggcgcg ccgggtttcg ccttgccgtt cgcgcgccgc
     1981 cacgcgtcga cctcccgcca ggtcgcgaag aggcccggcg cgccggggta gtaatagccg
     2041 aagtcgaggg ccggagtggg atcgggcacg tcgagacccg gcacgccggc cgccgacagc
     2101 gcgtacttga gcaagccgag ctggttctcg aggcccgaat tcgcccacaa cgctcgagcg
     2161 cgatcgtccc acgtcacgcc ttgcgcgagg tagctttcct tcggcagcag cccttcgccg
     2221 acgccgatca cgctgccgcg ctcgttgatg cgctcgatca ggttcgtgtc gtgctcggtg
     2281 ttgaaccgat cgaccatctg ctgattcatg acgtcgagca cgagcacgtc ggagcgctcg
     2341 gccttcgcgg catcgacgtc gtcgaagacg gattccgtga cgaacccgag ctcgacgcga
     2401 tcgcgcagct cggggtgctg ctcgagcagc tccttgtagg ccgcgagcgt cacgggcatc
     2461 tgcccgtcgg agaagagata ggtcacgcgc acgcgatcct gcgcgctcgc cggcaacgcc
     2521 gcgaagagca ggacgagcga gatgagccaa cgccgcggcg tcgtcacggc ggcggcgcct
     2581 cgttgtccgg cacgtagatg atctggccgt tcgcgaggcg gtacgccgtg ccgaggcgct
     2641 cgccgtcgcc cgcgagctgc tcgcccgtga ctttgtaggc ctcgatctcc gtgcccttct
     2701 tgatgatgac ttcgctctcg ccttgcgcgt tcgtcttgac catcgtgact tccgagtcgg
     2761 ggtcgatgag gtcgcgcatg cggtagatcg agaacaggct gatcatgagg ccgacgatga
     2821 acacgaccga cacgtcgaac aggttcgcga cgcccgcgag cgggtcttcg tcctgctcgt
     2881 cggggtcggc gagcttcaaa aacttgctca tcgtcaggct ccttgcgcga gctcggccgc
     2941 gatgcgctcg gccaggaaac gttgctcgcg cacggcctcc gtgacccagt gctgccggat
     3001 cgtcaggatc acgtacgccg cggtgccgca ggcgagaccg atgatcgtcg tcgtgaacgc
     3061 gacgaccatt tgcccggcca tcgcatcgag gtttccggtc gagagcgccg cgagcgccgt
     3121 gcccatcggg atcagagtgc cgagcagccc caagctcggg ccgaccttca cgagcaagcg
     3181 cgagccgctc aggctcttgc gcgtgcgctc ctcgctcgcg agaaccaagt gctcgaggcc
     3241 gccgttcgcg aggcgcgcct cggcgcggcc gcgctcgacg tcggcgagca ggcgcttcat
     3301 ggccgccggc aattgcgcgc gccgcgctgc gtcggcgccg agcacgcgcc cctgcttcag
     3361 ccacgcttcg acgtcgaacc cgttgcgctc gcgccgccgt gcgagcgcct cgacgacggc
     3421 gctgcccgcc atgaacagca cggccagcac gcaaatccag agcagcgcca tgacgggaaa
     3481 gcgcagcacc tgacccaagg cgaacaagcc gttttcgaga agcccaagca aattcatcgc
     3541 cgtttccaaa tcgtgtaggc gccgccgagc agcgcagtga ctatgacgag cgcgccgagc
     3601 catggttcga tctgcggacc ggccgaggcc aacggctcgc tttgcagcgc gaccgatgac
     3661 cgccaacctt cgacgacgcc gggacccagg ccgagcagca gcgcgaacgc cgcgacgatc
     3721 acgagcgcct cgtcgcgcag gtcggcggtc acgaatccgc gccgcaacgc cgccagaacg
     3781 acggccgctg ccgtcacgaa gaccgcgacc gcggcggccg gcagccatgg taggccctga
     3841 gcctgcaaga tcgaaatcca ggccgctgcc gagatgccgg ccgcgagcgg cggcagcagc
     3901 cgccattgcg gccgagcgag cgccgcgacg gccgcggccg cgaggcagaa gccgagcgcc
     3961 gtcacgtcga gcgcgccgag gcgcgcgaat acgaacgcgg cg
//
LOCUS       DASSNN010000823         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_732188, whole genome shotgun sequence.
ACCESSION   DASSNN010000823 DASSNN010000000
VERSION     DASSNN010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732188"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2045
                     /locus_tag="VFL84_15430"
     CDS             <1..>2045
                     /locus_tag="VFL84_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HET7610073.1"
                     /translation="ALAQAPIVNPGAPGAAPRALGPDEAIALARTAYTSADVRFLQAM
                     IPHHAQALEMALLVKDRTNRKELLDAAGRIDASQKDEIEFMQKWLAERNEPAMDPAAP
                     TADHAAHAGHAGGMKGMATPEQMAALAAANGPEFDRQFLTLMIKHHEGAVEMVADLTE
                     QPGSAYEPALFEFTRDVTNDQNAEIERMNALLVGLSTDPRAALKAGFADAGEAISNLT
                     KVVSLPKPPGFFDPANPANLPPKRLAALAAADQAGGAKPADSAAKAAEKKDEPSGGGG
                     GGNNNRDPRSPLLSFANTDLAFRGDVMVVGSYHGFNVYRLRGDRAPELLSSVVCPGGQ
                     GDVSIVGDLVIMSVEQTRGRVDCGLEGVEVDASPDRFRGLRIFDISDLTRPVQVGQVQ
                     TCRGSHTHSVVASDERRIVVYNSGTAAVREEEELAGCIGAVAGDTRTALFRIDVIEIP
                     VRDPKRARIVDRPAVFADPATGVMAGLWRGGDHGDETQDTRATDQCHDITVFPTLGLA
                     AGACAGNGIVFDIRDPAKPFRIDAVVDKGFAYWHSATWNNDGTKVVFTDEWGGGSRPR
                     CRAHDRVDWGADAVYDLVDGKLVYRSHFKLPAPQVDEENCVAHNGSIVPVPGRDIMVQ
                     SWYQGGISVWDFTDSAAPVEIAYFDRGPVDAEELVLAGYWSSYWYDGRIYATEIQRG"
BASE COUNT          330 a          708 c          711 g          296 t
ORIGIN      
        1 cgcgctcgcg caagcgccga tcgtgaatcc cggcgctccg ggcgccgcgc cgcgcgccct
       61 tggccccgac gaagcgatcg cgctcgcgcg tacggcctac acgagcgccg acgtgcggtt
      121 cctgcaagcg atgattccgc accacgcgca ggctctcgag atggccttgc tcgtgaagga
      181 ccgcacgaac cgcaaggagc tgctcgacgc ggcggggcgc atcgacgcgt cgcagaagga
      241 cgagatcgag ttcatgcaga agtggctcgc cgagcgcaac gagccggcga tggatcccgc
      301 ggcgcccacg gccgatcacg ccgcgcacgc cggccacgcg ggaggcatga agggcatggc
      361 gacgccggag cagatggccg cgctcgccgc ggcgaacggc cccgagttcg accgccagtt
      421 cctgacgctg atgatcaagc accacgaagg cgccgtggag atggtcgcgg acctgacgga
      481 gcagccgggc tcggcgtacg agccggcgct gttcgagttc acgcgcgacg tgacgaacga
      541 ccagaacgcc gagatcgagc gcatgaacgc gctgctcgtc gggctctcga ccgatccgcg
      601 cgccgcgttg aaagccgggt tcgccgacgc cggcgaggcg atctcgaatc taacgaaggt
      661 ggtctcgttg ccgaagccgc ccgggttctt cgatcccgcg aatccggcga acttgccgcc
      721 gaaacggctc gcggcgctcg cggccgcgga ccaagccggt ggcgcgaagc cggccgactc
      781 cgcggcgaag gcggccgaga agaaggacga gccttcgggc ggcggcggtg gcggcaacaa
      841 caaccgcgac ccgcgctcgc cactcttgtc gttcgcgaac acggatctcg cgtttcgcgg
      901 cgacgtcatg gtcgtcggca gctaccacgg cttcaacgtc tatcggctgc gcggcgatcg
      961 agcgcccgag ctcttgtcgt cggtcgtctg ccccggcggc caaggcgacg tctcgatcgt
     1021 cggcgacctc gtcatcatgt ccgtcgagca gacgcgcggc cgcgtcgact gcgggctcga
     1081 aggcgtcgag gtcgacgcga gcccggaccg cttccgcggc ttacgcatct tcgacatctc
     1141 ggatctcacg agacccgtgc aagtcggaca agtgcagacc tgccgcggct cgcacacgca
     1201 ctccgtcgtc gcgagcgacg agcggcgcat cgtcgtctac aactccggca ccgcagccgt
     1261 gcgcgaggaa gaagagctcg cgggctgcat cggcgccgtc gccggcgaca cgcgcacggc
     1321 gctgtttcgc atcgacgtca tcgagattcc cgtgcgggat ccgaagcgcg cgcgcatcgt
     1381 cgatcggccc gccgtgttcg cggatcccgc gaccggcgtc atggccggcc tctggcgggg
     1441 cggcgatcac ggcgacgaga cgcaagacac gcgcgcgacc gatcagtgtc acgacatcac
     1501 cgtgtttccg acgctgggct tggccgcggg cgcgtgcgcc ggcaacggca tcgtgttcga
     1561 tatccgcgac cccgcgaagc cgtttcgcat cgacgccgtc gtcgacaagg gcttcgcgta
     1621 ctggcattcg gcgacgtgga acaacgacgg cacgaaagtc gtgttcaccg acgagtgggg
     1681 cggcggctcg cggccgcgct gccgcgcgca cgaccgcgtc gactggggcg ccgacgccgt
     1741 ctacgacttg gtcgacggca agctcgtcta tcgcagccac ttcaagctgc cggcgccgca
     1801 agtcgacgag gagaactgcg tcgcgcacaa cggctcgatc gtgcccgtgc cggggcgcga
     1861 catcatggtt cagtcgtggt accagggcgg catttccgtc tgggatttca cggactccgc
     1921 cgcgcccgtc gagatcgcgt acttcgatcg cgggcccgtc gacgccgagg agctcgtgct
     1981 cgcgggttat tggtcgtcgt actggtacga cgggcgcatc tatgcgacgg agattcagcg
     2041 cgggc
//
LOCUS       DASSNN010000824         3087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1749484, whole genome shotgun sequence.
ACCESSION   DASSNN010000824 DASSNN010000000
VERSION     DASSNN010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749484"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..339
                     /gene="hfq"
                     /locus_tag="VFL84_15435"
     CDS             97..339
                     /gene="hfq"
                     /locus_tag="VFL84_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007022969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HET7610074.1"
                     /translation="MAKSQSLQDPFLNALRKEKVPVSIYLVNGIKLQGQIDSFDQFVV
                     LLRNSVSQMVYKHAISTVVPARNVRIPAMDEEEAED"
     gene            372..1655
                     /gene="hflX"
                     /locus_tag="VFL84_15440"
     CDS             372..1655
                     /gene="hflX"
                     /locus_tag="VFL84_15440"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018860596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HET7610075.1"
                     /translation="MLDRPRSGERALLLHIGLTRPCFDDELEEFRALAESAGARVVGE
                     IHARRDRPDPKFFVGSGKVEELAARAQETQAELILVNQPLRAGQERNLELALKVRVLD
                     RNGLILDIFAQRAATFEGKLQVELAQHEHLASRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLVSRRIRHLRDRLERVERQREVSRKERQRSDLAVVALVGYTNAGKTTLFNALA
                     GANLVAKDQLFATLDPTVRRLPLGRGTEVLLIDTVGFVRDLPHELVAAFRATLTETRE
                     ASLLLHVIDASDPHHEDRQRQVEDVLADIGAAEVPSLRVYNKVDRAPPDFVRDFDGAE
                     ITKLCVSAITGEGLAALKDAIRERVAGARVAGELRLKPEQSRLRARLFDWHAVRSETV
                     EDDGGYTLDVDLPAQRWRQLREEEGLSADSIRQKV"
     gene            1662..2777
                     /gene="hflK"
                     /locus_tag="VFL84_15445"
     CDS             1662..2777
                     /gene="hflK"
                     /locus_tag="VFL84_15445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HET7610076.1"
                     /translation="MAWDDGDKGNPWRSDKDKGPADLDAVVRDLQRKLSGIFRGGAGR
                     GGEQGVSSGLTASALVIVMLVWASFGFYRVDAAERGLEFRFGAFQGLTQPGWQWHWPW
                     PIEHVEVVNTGQTATRPYRGSMLTRDENIVDVELLIQFRRTDPQAFTFNMRNPEEALD
                     YATASAIREVIGRNLLDFILTDGRAEVARDAQDLLQATLDSYGAGITIYEVNLVNANF
                     PSAVEGSVQDSIRAREDRARRILEASGYSNDVLPRARGEAARRRQDAEAYRAQVVADA
                     EGEADRFMQILAAYQKAPAVTRERMYIETLEEILRNSTKVLVDTDGGNNLLYLPLDQL
                     VQRRPLPSPTEQPSSNASPPTTPNTTARPRERERETR"
     gene            2791..>3087
                     /locus_tag="VFL84_15450"
     CDS             2791..>3087
                     /locus_tag="VFL84_15450"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET7610077.1"
                     /translation="MGPRLNLLLILAVAAVILIRMSVFTVDAREYALKFQVGRIVRAD
                     YQPGLHFKIPFFENVARYPKTILNYDEDSEERFLTGEKKNVIVDYFVTWRIVDPA"
BASE COUNT          566 a          965 c         1042 g          514 t
ORIGIN      
        1 atcggagttc gaggttttca ggcagcacag tgggccgatg cgacacggtc cgcgctggtc
       61 tcgccgatac caacaagaac aaccaggaga gcaggtatgg ccaagagtca gtcgcttcag
      121 gacccgtttc tgaatgcgtt gcgcaaagag aaggtgcccg tctcgatcta tcttgtgaac
      181 ggcatcaagt tgcaagggca gatcgactcg ttcgatcagt tcgtggtgtt attgcgcaat
      241 agcgtcagcc agatggtcta caagcacgcg atttccacgg tagtgccggc gcgcaacgtg
      301 cgcatcccgg ccatggacga ggaagaggca gaggactaga tagatgccgt tccctggccc
      361 agggaggtaa gttgctcgat cggccgcgca gcggcgaacg cgccctgcta ttgcacatcg
      421 gcctgacccg accgtgtttc gacgacgaac tcgaggaatt ccgcgcactc gcggaatccg
      481 cgggcgcccg cgtcgtcggc gaaatccatg cacggcgtga tcgcccggat cccaagttct
      541 tcgtcggcag cggcaaggtc gaagagctcg ccgcgcgcgc gcaagagacg caagccgagc
      601 tgatcctcgt caaccagccg ctgcgtgcgg gacaggagcg caatctcgag ctcgctttga
      661 aggtgcgcgt tctcgaccgc aatggcctga ttctcgacat cttcgcccaa cgcgccgcga
      721 cgttcgaagg caagctgcaa gtcgagctcg ctcagcacga gcacctcgca tcgcggctcg
      781 tgcgcggctg gacgcacctc gagcggcaaa agggcggcat cgggctccgc ggtcccggcg
      841 agacgcagct cgaaacggac cggcggctcg tgagccggcg gatccgtcat ttgcgggacc
      901 ggctcgagcg tgtcgagcgc cagcgcgagg tcagccgcaa ggagcgccag cgctccgact
      961 tggccgtagt cgcgctggtc ggctacacga acgccggcaa gacgacgctg ttcaacgcgt
     1021 tggccggggc gaatctcgtc gccaaggatc aattgttcgc gacgctcgat ccgaccgtac
     1081 ggcgcctgcc gctcgggcgc ggcaccgagg tcctgctcat cgacaccgtc ggtttcgtcc
     1141 gcgacttgcc gcacgagctc gtagcggcgt tccgcgccac gctgacggag acgcgggagg
     1201 cgtcgctgct gctacatgtc atcgacgcga gcgatccgca tcacgaggac cggcagcgcc
     1261 aagtcgagga cgtcctggcg gacatcggtg ccgccgaggt tccgtccctt cgggtgtaca
     1321 ataaggtcga tcgggcgcca cctgatttcg ttcgggattt cgacggcgcc gagatcacca
     1381 agctctgtgt ctccgccatt accggagaag gtcttgcggc gctcaaggac gcgattcgcg
     1441 agcgagtggc cggggcgcgc gtcgcgggag agctgcgact caagccggag cagagtcggc
     1501 tccgcgcgcg gctgttcgat tggcacgccg tgcgctcgga gaccgtggaa gatgacggcg
     1561 gctatacgct ggacgtggat ctgccggcgc agcgttggcg gcagctgcga gaagaggaag
     1621 gcttgtcagc cgacagtatt cgacagaaag tgtgatgcac gatggcttgg gacgatggcg
     1681 acaaaggcaa tccgtggcgg tctgacaagg acaagggtcc tgcggatctc gacgcggtag
     1741 tgcgcgacct gcagcgcaag ctctccggta tttttcgcgg cggcgcgggc cgcggcggcg
     1801 agcagggcgt gagcagcggg ctcacggcga gcgcgttggt gatcgtgatg ctcgtctggg
     1861 cgagcttcgg tttctatcgc gtcgatgcgg ccgagcgcgg cctcgaattc cgcttcggcg
     1921 cgttccaggg gctcacgcag ccgggctggc agtggcattg gccatggccg atcgagcacg
     1981 tcgaggtcgt gaacacgggg cagacggcga cgcggcccta ccgcggcagc atgctgacgc
     2041 gcgacgagaa catcgtcgac gtcgagctgc tgatccaatt ccgccgcacg gacccgcagg
     2101 cgttcacgtt caacatgcgc aatcccgagg aggcgctcga ctatgccacc gcgagcgcca
     2161 ttcgcgaggt catcgggcgc aacctgctcg acttcatcct cacggacggc cgcgccgagg
     2221 tcgctcggga cgcgcaggat ctattgcagg cgacgctcga ctcctatggc gcgggcatca
     2281 cgatctacga agtcaacctc gtcaacgcca acttcccgag cgcggtcgag ggcagcgtgc
     2341 aggactcgat ccgagcgcgc gaagatcgcg cgcgaagaat tctcgaggcg agcgggtact
     2401 cgaacgacgt gctgccgcgg gcgcgcggcg aggcggcgcg gcgccgccag gacgccgagg
     2461 cgtaccgggc acaggtcgtc gccgatgccg agggcgaggc ggaccggttc atgcaaatcc
     2521 tcgcggctta tcaaaaggcg ccggcggtga cgcgcgagcg catgtacatc gagacgttgg
     2581 aggaaatact ccgcaactcg acgaaggtgc tcgtcgatac ggacggcggc aataatttgc
     2641 tgtacctgcc gctcgatcag ctcgtgcagc gccgtccgct gccgtcgccg accgagcaac
     2701 cgtcctcgaa cgcctcgccg ccgaccacgc cgaatacgac ggcgcggcct cgcgagcgcg
     2761 agcgcgagac gcgttagcga ggatttgaga atgggcccac gtttgaactt gctgctgatc
     2821 ctcgccgtcg ccgcggtcat cctcatccgc atgtccgtgt tcaccgtcga cgcgcgtgag
     2881 tacgcgttga agttccaagt cggccgcatc gtgcgtgcgg attatcaacc cggcttgcac
     2941 ttcaagattc cgtttttcga gaacgtcgcg cgctatccga agacgatctt gaattacgac
     3001 gaggacagcg aggagcgctt cctgacgggc gagaagaaga acgtcatcgt cgattacttc
     3061 gtgacgtggc gcatcgtcga cccggcc
//
LOCUS       DASSNN010000825         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1369771, whole genome shotgun sequence.
ACCESSION   DASSNN010000825 DASSNN010000000
VERSION     DASSNN010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369771"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..624)
                     /locus_tag="VFL84_15455"
     CDS             complement(46..624)
                     /locus_tag="VFL84_15455"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HET7610078.1"
                     /translation="MVPAAVTRSLLGYGVLAGPFYLALGVAQGLIRDGFSFARHPLSV
                     LANGPGGWLQTANFALSASMVLAAVVGIARAAPQHSRALRAALVVYALGMIVAAVFRA
                     DPMDGFPPGTPLGVPTTLSTSGVAHFAAGAVTFLAMAIACFAGARTMSKRGEHGMARF
                     SLLAGLVVFVAFFGGSAFASSVGGVAASGSPS"
     gene            complement(665..>2591)
                     /locus_tag="VFL84_15460"
     CDS             complement(665..>2591)
                     /locus_tag="VFL84_15460"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610079.1"
                     /translation="RRTDTPDGPMDLPEYVREYREELVLKPNRGYGGAGVVLGAAVEQ
                     REWEALLGEALAAQNDPHRHWVVQAAATLPVHLFPVLDELGRTHEEPFYAVMGFAPTD
                     HGLGIIARVSQKHVVNVAQRGGLAAVLVGYRPEDLKSSTRSPVQADLATQELRALVQR
                     LRDLDGVIHLLEWDEETYRPRDAAEDRASQLAVLGALRHDLLAGDRLGDLLAALATRE
                     DATPRERAELERLGRLRRTAVALPQSLVAAFAETRSHCLAAWEEARRTDDYATFLGPF
                     AQLLKLMRERAEALKLGDTLYDGLLDEHEPGMRRTRLDPLLAKTGARLRELVPKLAER
                     TRRYAELLPRGGYHQVRQRSFCADLLHDMGFDFARGRLDRSTHPFTMMTSDDDVRLTI
                     RSIADDPLHAIFATLHEGGHALYDQGLPRELHGTLLADAPSMGVHESQARLWENHVGR
                     SAAFWRHYFPKLQAAFPAKLGELDARSFQRAINVVAPGVNRVSADEATYNLHVLVRYE
                     LEIALLDGDLAVTDLPGAWDERYRRYLGLSAALPRDGCLQDVHWALGEFGYFPTYTIG
                     NLYAAQLVDAYDENHDFDAELASGNLGALRAWLAENVYARGAELPAETLIERITGRTL
                     DVEPFFRRLERRVAELE"
BASE COUNT          419 a          908 c          912 g          352 t
ORIGIN      
        1 cgcgataaaa acggcgtgag acgatcgcga gccacgccca gcctgtcacg acggcgaccc
       61 agacgcggca acgccgccga cgctgctcgc gaacgcgctg ccgccgaaaa aggccacgaa
      121 cacgacgaga cctgcgagca acgagaatcg cgccatgccg tgctcgcctc gtttcgacat
      181 cgtgcgcgcg cccgcgaagc aggcgatcgc cattgcgagg aacgtaaccg caccggcggc
      241 gaagtgcgcg acgcccgacg tcgacagcgt cgtcggcacg ccgagcggcg tgccgggagg
      301 aaagccgtcc atcggatcgg cgcgaaagac ggccgcgacg atcatgccga gcgcatagac
      361 gacgagcgcg gcgcgcagcg cgcgcgagtg ctgtggcgcc gcgcgcgcga tgccgacgac
      421 ggccgcgagc accatcgacg cgctgagcgc gaaattggcc gtctgcagcc agccgcccgg
      481 cccgttcgcg agcacgctca agggatgccg cgcgaagctg aacccgtcgc ggatgagtcc
      541 ctgcgccacg ccgagcgcga gatagaacgg cccggcgagc acgccgtagc cgagcagcga
      601 acgagtgacg gcggccggta ccatatgcgt agcctcgctg agagatcagt tcaattgcgc
      661 ggcgctactc gagctcggcg acgcgccgct cgaggcgcct gaagaacggc tcgacgtcga
      721 gcgtgcggcc cgtgatgcgc tcgatcagcg tttcggccgg cagctccgcg ccgcgcgcgt
      781 agacgttctc ggcgagccac gcgcgcagcg cgcccaagtt gccgctcgcg agctcggcat
      841 cgaagtcgtg attctcgtcg taggcgtcga cgagctgcgc cgcgtacagg ttgccgatcg
      901 tataggtcgg gaaataaccg aactcgccga gcgcccagtg cacgtcttgc aggcagccgt
      961 cgcgcggcag cgccgcgctt agacctaagt agcggcggta gcgctcgtcc caggcgcccg
     1021 gcagatccgt gacggcgagg tcgccgtcga gcaacgcaat ctcgagctcg tagcgtacga
     1081 gcacgtgcaa gttgtacgtc gcctcgtcgg ccgagactcg gttgacgccg ggcgcgacga
     1141 cgttgatggc gcgctgaaag ctgcgcgcgt cgagctcgcc cagtttcgcg gggaatgccg
     1201 cttgcagctt cgggaagtaa tgacgccaga acgccgcgct gcgcccgacg tgattctccc
     1261 agagccgcgc ctgcgactcg tgcacgccca tgctcggcgc gtccgcgagc aacgtgccgt
     1321 gcagctcgcg cggcagcccc tggtcgtaga gcgcgtggcc gccttcatgc agcgtcgcga
     1381 agatcgcgtg cagcgggtcg tccgcgatcg agcggatcgt gagccgcacg tcgtcgtcgc
     1441 tcgtcatcat cgtgaacggg tgcgtcgagc gatcgaggcg gccgcgcgcg aagtcgaagc
     1501 ccatgtcgtg gagcaaatcg gcgcagaagc tgcgttgccg cacttgatgg tagccgccgc
     1561 gcggcagcag ctcggcgtag cggcgcgtgc gctcggccag cttcggcacg agctcgcgca
     1621 agcgcgcgcc ggtcttcgcg agcagcggat cgagccgcgt gcgccgcatg ccgggctcgt
     1681 gctcgtcgag caggccgtcg tagagcgtgt cgccgagctt gagcgcttcg gcgcgctcgc
     1741 gcatgagctt cagcaattgc gcgaacggac ccaaaaaagt cgcgtagtcg tcggtgcggc
     1801 gcgcctcctc ccacgccgcg aggcaatgcg agcgcgtctc ggcgaacgcc gcgacgaggc
     1861 tttgcggcag cgcgacggcc gtgcgccgca accggccgag ccgctcgagc tcggcgcgct
     1921 cgcgcggcgt cgcgtcctcg cgcgtcgcga gcgccgcgag caagtcgccg agccggtctc
     1981 cggccaaaag atcgtgccgc aacgcgccga gcacggcgag ctgcgacgca cggtcctcgg
     2041 ccgcgtcgcg cgggcgatac gtttcctcgt cccattcgag caggtgaatg acgccgtcga
     2101 gatcgcgcag gcgctgcacg agcgcgcgca gctcctgcgt cgcgaggtcg gcctgcacgg
     2161 gcgagcgcgt cgagctcttc agatcctcgg ggcggtagcc gacgagcacg gcggcgagac
     2221 cgccgcgctg cgcgacgttg acgacgtgct tctgcgagac gcgcgcgatg atacccaagc
     2281 cgtgatccgt cggcgcgaag cccatcacgg cgtagaacgg ctcctcatgc gtgcggccga
     2341 gctcgtcgag cacggggaaa agatgcacgg gcagcgtcgc cgcggcctgc acgacccagt
     2401 gccggtgcgg atcgttttgc gcggccagcg cttccccgag cagcgcttcc cattccctct
     2461 gctcgacagc agcgcccaac acgacgccgg cgccgccgta gccacggttc ggcttcagca
     2521 cgagctcctc gcggtactcg cgcacgtact cgggcaggtc catcggcccg tccggcgtgt
     2581 cggtgcgccg c
//
LOCUS       DASSNN010000826         8568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1750619, whole genome shotgun sequence.
ACCESSION   DASSNN010000826 DASSNN010000000
VERSION     DASSNN010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8568
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750619"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VFL84_15465"
     CDS             <1..1013
                     /locus_tag="VFL84_15465"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HET7610080.1"
                     /translation="DAAAEREAIEAALALDAYFLPALLAKGSWFERFGTPAGAAATFA
                     NALRVAPPPSQVPAALQRELDHAREVVDAHANALDRHLALELGPLLTQLPAGTAARWR
                     EAASIVAGKSKPYTSQCNQLQVPRLPAIPFFDRAQFPFLTELETKTDVIRAELEAALA
                     SDRDKFTPYIQYAPGQPVNQWQELNHSLRWSALHLWRSGKPVEENLARCPETARALAA
                     LPLAGIEGLCPNAMFSALAPKSHIPPHNGETNARVVAHLPLIVPAGCRYRVGYDWCEW
                     RVGEALIFDDTIEHEAYNDSDELRVVLIFDLWNPLLTPAEREVVAALASAARTFAQVD
                     AR"
     gene            1118..4201
                     /locus_tag="VFL84_15470"
     CDS             1118..4201
                     /locus_tag="VFL84_15470"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7610081.1"
                     /translation="MRITNVLSMAIRRELALASLLLPALSSAQEQPNQAEQIVVTGTR
                     LANPNLVSTSPIQVVTSEEIEISGRNDVSDILQLLPQNFSNDLGQDLGNRTSGLTTAG
                     GVATADLRGLGPNRTLVLVNGRRLGNGSPYTVIQSPAPDLDQIPSRLIERVEVVTGGA
                     SAVYGSDAVAGVVNFITKRDFEGVEFEMQAGYNSHHNDNATAQALAADAGFDVPTGTV
                     SDGQTRNYNIIGGVNSGDGRGNITLFLGYQTQDGVRSDFRDFGAGQLFTDTDDNGVPT
                     GTVSMGGSSNSNFFQPKSGPNGEDPTAVFSVFGDQFVPFGTPGTTPPAFFNAQKDIYM
                     SRQFKRANAGLIGHYDLNDSVRPYVEFNFMNDKTHQEIAPSALFRQSNPLSDDGNYLI
                     NCSNPLLSAQQQSILCSPAEIAADAGDPGSALASVEIGRRNIEGGGRQSDYEHTNYRA
                     LAGLTGDFGKAWSYDGYGQYYYVQFYNSNNRYLNFERITNALQVQTGADGAPECISGP
                     PCVPYNIFSDGGVTQEALDYLYSSGTAYGTTTLRTAHLDFTADLGEYGIRIPSSSEGL
                     SLNVGYESREDAVDFLPDEASLSGLLSGFGGASVAIDESVDVDEYFAEVRVPLIQDKR
                     GVADLSLDFGARSSDYSTSGSVTTSKFEIQYAPVRSTRIRASFNNAIRAPSIVELFNP
                     NLVGQITIGEDPCAPTFDAAFNVIPAVNTLEECLRTVRPDQAAAFTAAYGNGGTTNQI
                     PQGTASQLSQLQGGNVNLRPEEADTYSVGVSFTPERIDRLNASIDYWNVDISDAIGTL
                     PAGVILNGCPNTGDPVFCTQLSRHPTTFSLQGASVEGGGYIIQTNQNIAASESSGIDV
                     QATYGLDFERRGSLLFALAGSYMLSNETTPYPGAHTYDCTGLFGLTCQTVNPEWRHVA
                     RATWRLPERLSATLSWRYIGAVKEDNNDPDPTLMNSAFAGFDPFNAEIGAQSYFDLAA
                     TLEVKQMELRAGINNITDKAPPLLGSEVVGGGSPNTYSTYDLFGRQLFFAVNVGF"
     gene            4217..4780
                     /locus_tag="VFL84_15475"
     CDS             4217..4780
                     /locus_tag="VFL84_15475"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET7610082.1"
                     /translation="MKLTAILVAGCFTGAALGAAAQTRGAAPSQDEGIVTAEAAPRAP
                     TDSASSRSIEFLLEALRGSLAEIRMGKLAAQQSYDQNVRAYGMKLETDHTAHAAEIER
                     LLTPLGVAIPTEPAGEAVAQLAALTRLSGKELDMAFVQMAVWTHTDAIEQYGAQTHAN
                     PDRAVHDFAARSLPMLREHLAQAEALR"
     gene            complement(4815..5879)
                     /locus_tag="VFL84_15480"
     CDS             complement(4815..5879)
                     /locus_tag="VFL84_15480"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7610083.1"
                     /translation="MHKVNSFFTLGVAAAAAAFASAALAGGEAGKLELRDVGARFVGY
                     TTKSAEGGSVDVLNPMFVQYLLPARRRHEHPVVFIHGGGGQGTDWLETPDGRDGWADY
                     FVAAGWDVYVVDRPGHGRSQSNGSCGNGQVRVGNSAIISRLSTSAPTVWPGGAPTPTN
                     DAVIGWTASSATAPYCGDALAAQTISALLDQIGPSVLVAHSAGGGSTFRVPDLNPDKV
                     VGIIAFEAAGSNPTQGGFNNSPALITTWKTEPALPAGFTAVDKNGCAMQGDRPSRLVH
                     YADLPIVLVSTEMGLMNKAAIECAASVWQQAGAKASAVYFPDRGLKGGGHFAMAQLDT
                     DKYAKIFLELAAQIESAAKK"
     gene            complement(5975..7945)
                     /locus_tag="VFL84_15485"
     CDS             complement(5975..7945)
                     /locus_tag="VFL84_15485"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET7610084.1"
                     /translation="MKRQGRGSWLRAASGLAAACWLSSLALAARADADEGDAAVREQF
                     RAAYAAAALGVETADDEALRAYVLYPYLRAARLDRALARAQGQWHEADDAAAEFLAQA
                     GDAPVALPLRRAWLSSLARRESWEAFLAQYETAATTPELECQRLNARIARQDTTGLAD
                     DIRARWLSDYRLPSACEAAFQWLRAQGGLPAELVARRVELLLDSGQASFARTIAARLP
                     AEAAAPLLERAQFIESPARMLDAFVRDDRKDVPAPVVLEAWSRLARNAPDEAHARYRD
                     LYGRMKREDARALAQPLAKGMAWDRRAQALDYFERVPNGTLDAVGREWWARAAMWAGK
                     WDEVRAAIAAMRPAEQADWPWLYWAARAAEEKGDKAAARSLYSAALPGDNYYSAMAAA
                     RLGERVVPRLEPIPVDAAQVESIAAVDAFRRVRELALLGLRELATNEWHYGYAVLPEA
                     QRLQAIHLAARWEIYDVAVALATSHGRFNDYTLLYPRPHLDEVAAAAKLTDVDVDLLY
                     GVLRQESLFRSDAASSAGALGLAQLTPATARETARRWALPAPNRADLFDPKISITLGA
                     ARLKELEQRFGSELPVALGAYNAGEAAAERWLPPRAVDSDVWIENIPYNETRAYVRRV
                     LWHGLVYRWLTTGQPQSTRDWLAKVASASTAP"
     gene            8109..>8568
                     /locus_tag="VFL84_15490"
     CDS             8109..>8568
                     /locus_tag="VFL84_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610085.1"
                     /translation="MFGPSTRAVALAAIGVLALPTIATAQGLERSRDIASSDVLSQIS
                     LFNYREHKRSELIFRATPVISTGEGSADIQYESGNANISAEVKRMPAPTLLGPYTTYV
                     LWALTPDGRASNEGVLTGAEGGSGSVETQHSASQFALIVTAEPHFAVSAPS"
BASE COUNT         1455 a         2979 c         2815 g         1319 t
ORIGIN      
        1 gcgatgcggc cgcggagcgc gaagccatcg aggccgcgct cgcgctcgat gcctacttcc
       61 tgcctgcatt gctcgcgaag ggcagctggt tcgagcgctt cggcacgccg gccggcgccg
      121 cggcgacgtt cgcgaacgcg ctgcgcgtcg cgccgccgcc gagccaagtt ccggccgcat
      181 tgcagcgcga gctcgaccac gctcgcgaag tcgtggatgc ccacgcgaac gctctcgacc
      241 ggcacctcgc tctcgagctc ggccccttgc tgacacagct gcccgccggc accgcagcgc
      301 gctggcgtga agcggcctcg atcgtcgccg gcaaatcgaa accgtacacc tcgcagtgca
      361 atcagctgca ggtgccgcgg ctgccggcca ttccgttttt cgaccgcgcg cagtttccgt
      421 tcctgaccga gctcgaaacg aagaccgacg tcatccgtgc agagctcgaa gcggcgctcg
      481 cttcggatcg cgataaattc acgccgtaca ttcaatacgc gcccggccag cccgtgaacc
      541 agtggcagga gctcaatcac tcgcttcgct ggagcgcgtt gcacctgtgg cgtagcggta
      601 agcccgtcga agaaaacctc gcgcgctgcc ccgaaacggc tcgcgcgctc gcggcgctgc
      661 cgctcgccgg catcgaaggg ctctgcccga acgcgatgtt ctcggcgctc gcgccgaagt
      721 cgcacatacc gccgcacaac ggcgagacga acgcgcgcgt cgtcgcgcac ttgccgctga
      781 tcgtgccggc cggttgccgc taccgcgtcg gctacgattg gtgcgaatgg cgagtcggcg
      841 aagcgctgat tttcgacgac acaatcgagc acgaggcgta caacgacagc gacgagctgc
      901 gcgtcgtgct gatcttcgat ctctggaacc cgttgctcac gcccgcggag cgtgaggtcg
      961 tcgcagcgct ggcgtcggcc gcccgcacgt tcgcacaagt cgacgcgcgc tagcctacaa
     1021 gtctctgttg cgtgatcgcc accggccgaa tgtggaaatt cgcgccgtgg tacatgactt
     1081 gcatgcgaat cgcaacacgc ccacgggggc tgcgaccatg cgaatcacta acgtcttgtc
     1141 gatggcgata cgtcgcgaat tggcgctcgc ctcactcctt ctgccggcgt tgtcctctgc
     1201 ccaggagcag ccgaaccagg ccgagcagat cgtcgtcacc ggcacccgac tggcgaaccc
     1261 gaacctcgtc agcacgagcc cgattcaagt cgtcacctcg gaagagatcg agatctcggg
     1321 ccggaacgac gtcagcgaca tcctccagtt gttgccgcaa aatttcagca acgacctggg
     1381 ccaagatttg ggaaacagga ccagcggttt gacgacggca ggcggcgtcg cgaccgccga
     1441 tctgcgcggg ctaggaccca accggacgct cgtgctcgtc aacggccggc ggctcggcaa
     1501 cggctcgccg tacacggtga tccagtctcc ggcgccggat ctggaccaga tcccttcccg
     1561 gctcatcgag cgcgtcgagg tcgtgaccgg cggcgcctcg gccgtctacg gctccgacgc
     1621 cgtggcgggc gtcgtcaact tcatcacgaa acgagatttc gagggcgtcg agttcgagat
     1681 gcaggccggc tacaactcgc accacaacga caacgcgacg gcgcaggcgc tggctgccga
     1741 cgccgggttc gacgtgccta cggggaccgt ttccgacggc caaacccgga attacaacat
     1801 catcggcggc gtcaacagcg gcgacggccg cggcaacatc acgctgttcc tcggttacca
     1861 gacccaggac ggcgtgaggt cggatttccg cgatttcggg gccggccagt tgttcacgga
     1921 caccgacgac aacggggtgc cgaccggcac cgtctcgatg ggcggatcga gcaattccaa
     1981 tttctttcag ccgaagagcg gtcccaacgg cgaggatccc actgcggttt tcagcgtctt
     2041 cggagaccag ttcgtgccgt tcggcacgcc aggcacgaca ccgccggcgt tcttcaatgc
     2101 tcaaaaagac atctacatgt cgcggcaatt caagcgcgcc aatgccgggc tgatcgggca
     2161 ctacgacctg aatgacagcg ttcggccgta cgtggaattc aatttcatga acgacaagac
     2221 gcatcaggag atcgcgccgt cggcactgtt cagacaaagc aacccgttgt cggacgacgg
     2281 caattacctc atcaactgca gcaatccgct gctgagcgcg cagcagcaaa gcattctgtg
     2341 ctcgccggcc gagatcgcgg ccgatgcggg cgacccggga tcggcgctgg cgagcgtcga
     2401 aatcggccgc cgcaacatcg agggcggcgg ccgccagtcc gactacgagc acacgaacta
     2461 tcgcgcgttg gctggcttga cgggcgattt cggcaaggcc tggagttacg acggctatgg
     2521 ccagtactac tacgtgcagt tctacaacag caacaaccgc tacctgaatt tcgagcgcat
     2581 cacgaacgcg ctgcaagtcc agaccggggc ggacggcgct cccgagtgca tcagcggccc
     2641 gccctgcgtt ccgtacaaca tcttttcgga cggaggcgtg acgcaagaag cgctcgacta
     2701 tctgtactcg agcggcacgg cttacggcac cacgacactg cgcacggcgc acctcgactt
     2761 cacggccgat ctcggcgagt acggcatccg cattccgtcg tcgagcgagg gcctctcgct
     2821 caacgtcggc tacgagagtc gcgaagacgc cgtcgatttc ttgcccgacg aggcctcgtt
     2881 gagcgggcta ctctctggat tcggcggcgc atcggtcgcg atcgacgagt ccgtcgacgt
     2941 cgacgaatac ttcgcggagg tgcgtgtccc gctgatccag gacaagcgcg gcgtcgcgga
     3001 cttgagcctc gacttcggcg cgcgcagctc cgactattcg acgagcggga gcgtgacgac
     3061 ctcgaagttc gagattcagt atgcgccggt gcgaagcacg cggattcgcg cgtcgttcaa
     3121 caacgcgatc cgcgccccga gcatcgtcga gctcttcaac ccgaacctcg tggggcagat
     3181 cacgatcggc gaggacccct gcgcgccgac gttcgatgcc gctttcaacg tgattccggc
     3241 cgtcaatacc ttggaggagt gtctgcgcac cgtgcggccg gatcaggccg cggcgttcac
     3301 ggcggcctac ggcaacggcg gcaccacgaa ccagataccc cagggaaccg cgagccagct
     3361 gtcgcagctg cagggtggaa acgtgaacct gcgccccgag gaggccgata cctacagcgt
     3421 cggcgtctcg ttcacgcccg agcgcatcga caggctcaat gccagcatcg attactggaa
     3481 cgtggatatc agcgatgcga tcggcacgct gcctgccggc gtcatcttga acggctgccc
     3541 caacaccggc gatccggtgt tctgcacaca gctgtctcgt caccccacga cgttctcgct
     3601 gcaaggcgcg agcgtcgaag gcggcggcta catcattcag accaatcaaa acatcgcggc
     3661 cagcgagtcc agcggaatcg acgtgcaagc aacctacggc ttggacttcg aacgccgcgg
     3721 ctcgctgctt ttcgcgctgg ccggctcata catgctcagc aacgaaacca cgccataccc
     3781 cggcgcgcac acgtacgact gcaccggctt gttcgggctt acttgccaga cggtgaatcc
     3841 cgaatggcgg cacgtcgccc gcgcgacctg gcggctgccg gaacgattgt cggccacgtt
     3901 gagctggcgc tacatcggcg cggtcaagga ggacaacaac gacccggacc cgactctgat
     3961 gaactccgcg ttcgccggtt tcgacccttt caacgcggag atcggcgcac aaagctattt
     4021 cgatctcgcg gcgacgctcg aagtgaaaca gatggagctc cgcgccggca tcaacaacat
     4081 cacggacaag gcaccgccgt tgctgggctc ggaggtcgtc ggcggcggct cgccaaacac
     4141 ctactcgacg tatgatttgt tcggccgcca gctgttcttc gccgtcaacg tcgggttctg
     4201 atcgagggag cgtcgaatga aactcacggc gattctggtc gcgggctgct tcaccggcgc
     4261 cgcgctcggc gccgcggccc agacgcgcgg cgcagcgcct tcgcaagacg aaggcatcgt
     4321 gacggccgag gcggcgccgc gagcgccaac ggacagcgca agctcgagga gcatcgagtt
     4381 tctgctcgag gcgctgcgcg ggagcctcgc cgagatccgg atgggcaagc tcgcggcgca
     4441 gcaaagctac gatcaaaacg tccgcgccta cgggatgaag ctcgagaccg atcacaccgc
     4501 gcatgcggcc gagatcgagc ggttgctcac gccgctcggc gtggcgattc cgaccgagcc
     4561 ggccggagag gccgtggcgc agctcgcggc gttgacgcgg ctgtcgggca aggagctcga
     4621 catggccttc gtgcagatgg cggtctggac gcacaccgac gcgatcgagc agtacggcgc
     4681 gcaaacgcac gcgaacccgg atcgcgccgt gcacgacttc gcggcgcgga gcttgccgat
     4741 gctgcgcgag cacctggccc aagccgaagc gctgcgctag ccgctacgcg cgctgcggcg
     4801 cgccgctcga ctccctattt cttcgccgcc gattcgattt gcgcggcgag ctcgaggaag
     4861 atcttcgcgt acttgtcggt gtcgagctgc gccatcgcga agtgaccgcc gcccttcagg
     4921 ccgcggtcgg ggaaatacac ggcgctcgcc ttcgcgccgg cttgctgcca gaccgaagcc
     4981 gcgcattcga tcgccgcttt gttcatgagg cccatctcgg tcgagacgag cacgatcggt
     5041 aaatccgcat agtgcacgag gcggctcggg cggtcgccct gcatcgcgca gccgttcttg
     5101 tcgacggccg tgaagcccgc gggaagcgcc ggctcggtct tccaggtcgt gatcaacgcg
     5161 gggctgttgt tgaagccccc ttgcgtcgga ttgctgccgg ccgcctcgaa cgcgatgatg
     5221 ccgacgacct tgtcggggtt cagatccggc acgcggaacg tgcttccgcc gcccgcggaa
     5281 tgcgctacga gcacggacgg gccgatctgg tcgagcagcg ccgagatcgt ttgcgccgcg
     5341 agcgcgtcgc cacagtacgg cgccgtcgcc gagctcgccg tccagccgat gacggcgtcg
     5401 ttcgtcggcg tcggcgcgcc gcccggccac accgtcggag ccgaagtcga aagccgcgag
     5461 atgatcgcgg aattgccgac gcggacctgg ccgttgccgc agcttccgtt gctctgcgag
     5521 cggccgtggc cgggccgatc gacgacatag acgtcccaac ccgcggcgac gaagtagtcg
     5581 gcccagccgt cgcgtccgtc cggcgtctcg agccaatccg tgccttggcc gccgccgccg
     5641 tggatgaaga cgaccggatg ctcgtgccgg cgccgcgcgg gcagcaggta ctgcacgaac
     5701 atcgggttca gcacgtcgac cgagccgccc tccgcgctct tcgtcgtgta gccgacgaac
     5761 ctcgcgccga cgtccctgag ctcgagcttg cccgcctcgc cgccggccag cgccgccgac
     5821 gcaaacgccg ccgccgccgc cgcgacgccg agggtgaaaa acgaattgac cttgtgcatt
     5881 cctgactccc gggtgacacc gatacgcgga acgacccgca agctgcggct ccggcgcatt
     5941 gtgctgatcg gcctccgcga aaagcaaccc gctctcaggg cgccgtcgag gcgctcgcga
     6001 ccttcgcgag ccagtcgcgc gtgctctgcg gctgccccgt cgtgagccac cggtagacga
     6061 gaccgtgcca gagcacgcgg cgcacgtacg cgcgcgtctc gttgtacgga atgttctcga
     6121 tccagacgtc gctgtcgacg gcgcgcggcg gcagccagcg ctcggccgcg gcctcgccgg
     6181 cgttgtaggc gccgagcgcg acgggcagct cggagccgaa gcgctgctcg agctctttca
     6241 gccgcgccgc gccgagcgtg atgctgatct tgggatcgaa gaggtcggcg cggttcggcg
     6301 ccggaagtgc ccagcggcgc gccgtctcgc gcgccgtggc cggcgtcagc tgtgccagcc
     6361 cgagcgcgcc ggcggaggaa gccgcgtcgc tgcggaacaa gctctcctgg cgcagcacgc
     6421 cgtatagcag gtccacgtcg acgtcggtca gcttcgcggc cgcggcgacc tcgtccaagt
     6481 gcggccgcgg gtagagcaac gtgtagtcgt tgaagcggcc gtggctcgtc gcgagcgcga
     6541 cggcgacgtc gtaaatctcc cagcgcgctg cgaggtgaat ggcctgcaag cgctgcgcct
     6601 ctgggagcac cgcgtacccg tagtgccact cgttcgtcgc gagctcgcgc aggccgagca
     6661 acgcgagctc gcgcacgcgc cgaaacgcgt cgacggcggc gatcgactcg acttgcgccg
     6721 cgtcgacggg gatcggctcg agccgcggca cgacgcgctc gccgagccgc gccgctgcca
     6781 tcgccgagta gtagttgtcg ccgggcagcg ccgcgctgta cagcgagcgc gccgcggcct
     6841 tgtcgccctt ctcctcggcc gcgcgcgcgg cccagtagag ccacggccag tcggcctgct
     6901 ccgccgggcg catcgcggcg atcgccgcgc gcacttcgtc ccacttgccg gcccacatcg
     6961 cggcgcgggc ccaccattcg cggcccacgg catcgagcgt gccgttcggc acgcgctcga
     7021 aataatcgag cgcctgggcg cgccggtccc aggccatgcc tttcgcgagc ggctgcgcga
     7081 gcgcgcgcgc gtcctcgcgc ttcatgcggc cgtagagatc gcggtagcgc gcgtgcgcct
     7141 cgtccggcgc attgcgcgcg agccgcgacc acgcctcgag cacgaccggc gccggcacgt
     7201 ctttgcggtc gtcgcgaacg aacgcgtcga gcatgcgcgc agggctctcg atgaattgcg
     7261 cgcgctcgag cagcggcgcg gccgcttcgg cggggaggcg cgccgcaatg gtccgcgcga
     7321 acgacgcttg gccgctgtcg agcaagagct ccacgcgccg cgcgacgagc tcggcgggca
     7381 gcccgccctg cgctcgcagc cattgaaacg ctgcctcgca cgcgctcggc aagcgatagt
     7441 cgctgagcca gcgcgcgcgg atgtcgtccg cgagaccggt agtgtcctga cgcgcgatgc
     7501 gcgcgttgag ccgctggcac tcgagctccg gcgtggtcgc cgccgtctca tattgcgcga
     7561 gaaacgcttc ccacgactcg cggcgggcga ggctcgagag ccacgcgcgg cgcagcggca
     7621 acgcgaccgg cgcgtcgccg gcctgcgcga gaaactcggc cgctgcgtcg tccgcttcgt
     7681 gccactgccc ttgcgcgcgc gcgagcgcgc gatcgagccg agccgcacgc aggtatggat
     7741 agagcacgta ggcgcgcagc gcctcgtcgt cggcggtctc gacgccgagc gccgcggccg
     7801 cgtaggccgc acggaactgc tcgcgaacgg cggcgtcgcc ttcgtcggcg tccgcccgag
     7861 ccgcgagcgc gagcgacgaa agccagcaag cggcggcgag cccgctcgcg gcgcgaagcc
     7921 aagatccacg tccctgtcgc ttcatgcgcg gaaggataca ccgtcgcgta tcgcgcgcat
     7981 taagtcacaa gcagcgcaga acgaggccgg gcgcggctcg aggccgatca aaaccgtgac
     8041 tggtgccaca attcgggctc cccggggcct tgcataattc cctaccacag ttgcgttagg
     8101 ggattgccat gttcggacct tcgacgcgtg cggtggcgtt ggccgcaatc ggggtgctcg
     8161 ccctgccgac gatcgcaacg gcacaaggac tcgagcgctc gcgcgacatc gcgagctccg
     8221 acgtgctgag ccagatctcg ctgttcaatt accgagagca caagagatcg gagctgatct
     8281 tccgcgccac gcccgtcatc tcgacgggcg aaggcagcgc cgacatccaa tacgagagcg
     8341 gcaacgcgaa catctcggcc gaggtcaagc gcatgccggc gccgacgctg ctcggcccgt
     8401 acacgaccta cgtgctgtgg gcgctgacgc ccgacggccg cgccagcaac gaaggcgtgc
     8461 tgacgggcgc cgagggcggc agcggctccg tcgaaacgca gcactcggcc tcgcagttcg
     8521 cgttgatcgt cacggccgag ccgcacttcg cggtcagcgc gccgagca
//
LOCUS       DASSNN010000827         3572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1751012, whole genome shotgun sequence.
ACCESSION   DASSNN010000827 DASSNN010000000
VERSION     DASSNN010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751012"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFL84_15495"
     CDS             complement(<1..345)
                     /locus_tag="VFL84_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC exporter domain-containing protein"
                     /protein_id="HET7610086.1"
                     /translation="MSQSIAGALLYLRVTTIKGWFAARLRRLKQPKYAVGAVIGVLYI
                     YSFFIRNVMQNGPRRPGTGPQAEALSFVPEIMALGLLVVIALNWIVPRGRAGLTFSEA
                     EISFLFPAPIKRS"
     gene            complement(345..1076)
                     /locus_tag="VFL84_15500"
     CDS             complement(345..1076)
                     /locus_tag="VFL84_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7610087.1"
                     /translation="MIEVTGLSKVFGTFTAVRDLSFTVRPGEVLGLVGPNGAGKTTTL
                     RAIAGIIPPTTGKVSLAGHDLLTAPAEAKRALAFFTDEPRLFDYLTVRQHLNFVARLY
                     AVADHDEVARPLLEELEIADRIDSLPAELSRGMKQKLAIACGLLHSPQVLMFDEPLTG
                     LDPLGIRRMKDSILKRAKAGAAIILSSHLLHLLEEVCSHVLILKRGEKLADGTLAEIR
                     ARFGDGDENANLEDVFIRATGGSER"
     gene            complement(1166..2029)
                     /locus_tag="VFL84_15505"
     CDS             complement(1166..2029)
                     /locus_tag="VFL84_15505"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET7610088.1"
                     /translation="MTIALGVDSLCWHMRLASGAITVERVLEQAASLEVPVVALNLHH
                     VRERSVPQLRELRGRARSLGLRLLAQGDFVGSPRRGDAVSAGVARIDAWVERAAALES
                     PSLRLVSGFYRAELAGQPALIEAERRYVGDVLARAAERAIAADVRLLLENHSDFTVDE
                     YERIVRDVGRDRMGVFLDLINPIVTFDDPMRAIERLAPLAQSGHVRDFVLRSIQQPDH
                     YHRRGFEVLYRYPGEGVAPIAALVTKLGSVVGDRPYDLVVEGLDSRAEVDDQVERLRP
                     ALAYLRRLPTE"
     gene            complement(2090..3550)
                     /locus_tag="VFL84_15510"
     CDS             complement(2090..3550)
                     /locus_tag="VFL84_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7610089.1"
                     /translation="MIRLSLSARLSLVFCALLLASSAAAAWLTVRANRLLEQELVQRL
                     SSDVAEHIADSAEFMDENGLRPDAVRILFDQLMSVNPSVEVYLLDGTGTIVGHAAPVG
                     HVRREQVAVEPIERLLAGAPLPILGDDPRSVGGRKVFDAARLTLAGRPWGYVYVILMG
                     ETRDVLAADVAAATVLRNTLVSLAAIAVVAVLLGLVAFRLITRPLRGLASAMQELDVD
                     RLAAAGAPAVADVRDERDEIGILSGAFGRMAQRIADQWRTLRRQDQERREIIANVSHD
                     LRTPLTSLHGFLETLAHKRDLSEAERERYLTTALAQSGKVSRLAHELFELARIEHDAS
                     SLEREPFSLGDLVHDVVQVLGVLAAAKEPRLTAQVPANLPQVVGSLRLIDRALTNLIG
                     NAIEHTPGGGAIVVTLRDLDRAVEVVVRDDGPGIPREVREALLSAPTGGSRAAGSGLG
                     LQIVRRILELHGSRIELDDAQGGTRIRFELPARALG"
BASE COUNT          562 a         1234 c         1263 g          513 t
ORIGIN      
        1 gctgcgcttg atcggcgccg ggaacaggaa cgagatctcg gcctcgctga aggtgaggcc
       61 cgcgcggccg cgcggcacga tccaattgag cgcgatcacg acgagcagcc cgagcgccat
      121 gatctccggc acgaacgaca gcgcctcggc ctgcgggccg gtgcccggcc ggcgcggccc
      181 gttctgcatg acgttccgaa tgaagaacga gtagatgtag aggacgccga tgacggcgcc
      241 gacggcgtac ttcggctgct tgagccttcg caaccgcgcc gcgaaccagc ccttgatcgt
      301 ggtgacgcga aggtacagca gcgcgcctgc gatcgactgg ctcatcagcg ctcgctgccg
      361 cccgtcgcgc ggatgaagac gtcctcgaga ttcgcgttct cgtcgccgtc gccgaagcgc
      421 gcgcggatct cggccagcgt gccgtccgcg agcttctcgc cgcgcttcaa gatcagcacg
      481 tgcgagcaga cctcctcgag caagtgcagc aggtgcgagc tcaggatgat cgcggcgccg
      541 gccttcgcgc gtttcaagat cgagtccttc atgcgccgaa tgccgagcgg gtcgaggccc
      601 gtcagcggct cgtcgaacat cagcacttgc ggcgaatgca gcaggccgca cgcgatcgcg
      661 agcttctgct tcatgccgcg cgagagctcg gccggcaggc tgtcgatgcg gtccgcgatc
      721 tcgagctcct cgagcagcgg ccgcgcgacc tcgtcgtgat ccgccacggc gtagagccgc
      781 gcgacgaagt tcaggtgctg gcgcacggtc aggtaatcga agagccgcgg ctcgtcggtg
      841 aagaacgcga gcgcgcgctt cgcctcggcc ggcgccgtga gcaggtcgtg gccggcgagg
      901 ctcaccttgc ccgtcgtcgg cggaatgatt ccggcgatcg cgcgcagcgt cgtcgtcttg
      961 cccgcgccgt taggaccgac gagcccgagc acctcgccgg ggcgaaccgt gaacgacagg
     1021 tcgcggacgg ccgtgaacgt gccgaagacc ttggacagtc ccgtgacttc gatcacctac
     1081 gctttccccc ggatgttgca tgcgcggcag ccgtcgcgag ctggccgcta ctctaagcgg
     1141 ggtcgtgttg ccggcgccag cccgatcact cggtcggcaa gcggcgaaga taggcgagcg
     1201 cgggccgaag gcgctcgacc tggtcgtcga cttcggcgcg gctgtcgagc ccttcgacga
     1261 cgaggtcgta cgggcgatcg ccgacgaccg acccgagctt cgtcacgagc gcggcgatcg
     1321 gcgcgacgcc ttcgcctgga tagcggtaca agacctcgaa gccgcggcga tggtagtggt
     1381 cgggctgctg aatcgagcgc agcacgaagt cgcgaacgtg gccgctctgc gcgagcggag
     1441 cgaggcgctc gatcgcgcgc atggggtcgt cgaacgtcac gatcgggttg atgagatcga
     1501 ggaacacgcc catgcggtca cggccgacgt cgcgcacgat gcgctcgtac tcgtcgaccg
     1561 tgaagtcgga gtgattctcg agcagcaagc gaacgtcggc ggcgattgcg cgttcggcgg
     1621 cgcgcgcgag aacgtcgccg acgtagcgcc gctccgcttc gatcaatgcg ggttggccgg
     1681 cgagctcggc ccgatagaag cccgatacga gccgcaacga cgggctctcg agcgccgccg
     1741 cgcgttcgac ccacgcgtcg attcgcgcga cgccggcgga gacggcgtcg ccgcggcgcg
     1801 gcgagccgac gaagtcgcct tgggccaaga gccgcaggcc gaggctccgc gcgcggcccc
     1861 gcagctcgcg aagctgcggc acggatcgtt cgcgcacatg gtgcagattc agcgcgacga
     1921 ccggcacttc aagcgacgcc gcctgctcga gcacgcgctc gaccgtgatc gcgcccgagg
     1981 cgagacgcat gtgccagcag aggctgtcga cgccgagcgc gatcgtcata ttcgccgacg
     2041 gctgcacgac gcatgccagc cgtgcgctcg gcggatgggc cgttgcgccc tatccgagag
     2101 ctcgcgccgg cagctcgaag cggattcgcg tgccgccttg cgcgtcgtcg agctcgatgc
     2161 ggctgccgtg cagctcgaga atgcgccgca cgatctgcag gccgaggccc gagcccgcgg
     2221 cgcgcgagcc gcccgtcggc gcggacaaca aagcctcgcg gacctcgcgt ggaatgccgg
     2281 ggccgtcgtc gcggacgacg acttcgacgg cgcggtcgag atcgcgcagc gtcacgacga
     2341 ttgcgccgcc gcccggcgtg tgctcgatcg cgttgccgat caggttcgtc agcgcgcggt
     2401 cgatcaagcg cagcgagccc acgacttgcg gcagattcgc cggaacctgt gccgtcagcc
     2461 gcggttcttt cgcggccgcg agcacgccga gcacttgcac gacgtcgtgg acgaggtcgc
     2521 cgagcgaaaa cggctcgcgc tcgagcgacg acgcgtcgtg ctcgatgcgc gcgagctcga
     2581 acagctcgtg cgcgagccgg ctgaccttcc cgctttgcgc gagcgccgtc gttagatacc
     2641 gttcgcgctc ggcctcgctc aagtcgcgct tgtgcgcgag cgtctcgagg aagccgtgca
     2701 gcgacgtgag tggagtgcgc aggtcgtgcg agacgttcgc gatgatctcg cgccgctcct
     2761 gatcctggcg ccgcagcgtg cgccattggt cggcgatgcg ctgcgccatg cggccgaacg
     2821 cgccgcttaa gattccgatc tcgtcgcgct cgtcgcgaac gtcggcgacc gccggggcgc
     2881 cggctgcggc aagccggtcg acgtcgagct cctgcatcgc gctcgcgagg ccgcgtagcg
     2941 ggcgagtgat caggcggaat gcgacgaggc ccaagagcac ggcgacgacg gcgatcgcgg
     3001 ccagcgagac cagcgtgttg cggagcacgg tggccgccgc gacgtcggcc gcgagcacgt
     3061 cgcgcgtctc gcccatcagg atcacgtaga catagcccca cggccggccc gcgagcgtaa
     3121 gacgcgcggc gtcgaagacc ttgcggccgc caacgctgcg cggatcgtcg cccaagatcg
     3181 gcagcggcgc gccggcgagc aggcgctcga tcggctcgac ggccacttgc tcgcgccgca
     3241 cgtggcctac cggcgcggcg tggccgacga tcgtgcccgt gccgtcgagc agatacacct
     3301 cgacgctcgg gttcacggac atgagctgat cgaacaggat ccgcaccgca tcgggccgca
     3361 gcccgttctc gtccatgaac tcggcgctat ccgcgatgtg ctccgcgacg tcgctcgaca
     3421 ggcgctgcac gagctcctgc tcgagcagcc ggttcgcgcg gaccgtgagc cacgcggccg
     3481 ccgcgctgct cgcaagcagc agcgcgcaga acacgagcga cagccgcgcg gagagactga
     3541 gccggatcac gttccgttcc cgtcggccga ca
//
LOCUS       DASSNN010000828         3792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1752419, whole genome shotgun sequence.
ACCESSION   DASSNN010000828 DASSNN010000000
VERSION     DASSNN010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752419"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFL84_15515"
     CDS             <1..221
                     /locus_tag="VFL84_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610090.1"
                     /translation="GYQTFYTNYEDGWAKKASAIFAPYERLPPDRPQSVPYAPFPEAF
                     VPPFHYRNPVSGRADHYKDPTWQDTQAP"
     gene            355..999
                     /locus_tag="VFL84_15520"
     CDS             355..999
                     /locus_tag="VFL84_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610091.1"
                     /translation="MSSEPSPDWQAPSGKRIALSVAVAIVIAAVVLVVAVLPAEYGID
                     PTGIGRLLGLDELKAPSTRTIEVKDVLGGNERVREVEIPAFNEPVPLPNPAVHQSEER
                     PIQTRTITLTLKAGQETEIKTVLRTNKVIVYTWHSEGGPAYCDLHGHDPAAGSEFFVR
                     YREDQEGATDVTGSLVAPFDGEHGWYWLNINEVPVTITLTVTGFFDDIVDYGIH"
     gene            1015..2625
                     /locus_tag="VFL84_15525"
     CDS             1015..2625
                     /locus_tag="VFL84_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005990096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HET7610092.1"
                     /translation="MWQQNYDPLGNFPLSTLVAALPIFVLLLLIGILRKPAWMSALAG
                     LGTALVVAIVAYGMPANLAVSASLMGAAFGLLPIGWIVYWAVVLYRVTLETGNFEIIK
                     DSIGGLTTDRRLQAMLIAFAFGAFVEGAAGFGTPVAVAAAMLTGLGFSPFFAAGICLL
                     ANTAPVAFGSIGIPVTTLANTTGLPEAELSAWVGRICAPISVFIPAYLVMVMGGLKGL
                     KGVLPAAIVVGVCFASVQLTVSNLIGPQLVDILAALTAIVGLVVLLKVWQPKDHFVLA
                     GEHAAVAVPKHYPLGRTLLAWSPYLLLVAFVLVWGYPDTKTILARASIPIPWPGLHNA
                     ITRMPPAVAAPSPYGAVYDFQWLVAAGTACAFATMASALVLRVSVAKYLGILGRVFKQ
                     LAFSLLTIAAVLGLAYVMNYSGAVVTLGLAFAATGALFPFFSSLVGWLGVFLTGSDTS
                     ANALFGNLQVVTANSLGFDPAVMAASNSAGGVMGKMISLTSLAVAVAATGMARSEEGK
                     LFRFTVKHSVFLASLLGIIATIYAYAYR"
     gene            complement(2651..>3792)
                     /locus_tag="VFL84_15530"
     CDS             complement(2651..>3792)
                     /locus_tag="VFL84_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7610093.1"
                     /translation="ASVSHELRTPLNAILGWAQVIAAGKVSDEDVKRGVETIVRNARA
                     QAQLIEDLMDMSRIASGKVRLDVRETDVPMVVQQAVETIRLSADSKAIRIRQIVDPSI
                     GPVSGDPNRLQQIVWNLLSNAVKFTPKAGNVDVIVQRVNSHVELTVRDSGVGIGQDFL
                     PHVFERFRQADSAPSRAYGGLGLGLSIVKHLVELHGGSVRAHSDGENRGATFVVTLPL
                     APVRSKEEWEHPTASKSLITKLENVDLEGVRVLVVEDERDSRELIKRVLEQYHADVRT
                     VGSAKEALEALVSSRPDVIISDIGMPDVDGYEFMRQVRRRTSELGGGVPAIALTAFAR
                     SEDRTRALMAGYQMHIAKPIEPHELVATVASFADWAHSHAHPHPE"
BASE COUNT          618 a         1269 c         1190 g          715 t
ORIGIN      
        1 acggctacca gacgttctat acgaactacg aggacggctg ggcgaagaag gcgtcggcga
       61 ttttcgcgcc ctacgagcgc ctgccgccgg accggccgca gtccgtgccg tatgcgccct
      121 ttcccgaagc gttcgtgccg ccgttccact atcgcaatcc cgtgtcgggc cgcgccgatc
      181 actacaagga tccgacgtgg caagacacgc aagcgccttg agaggggaca aatcataaaa
      241 ggggacagat ctatttttcg acgcccttgg cgcagcaaaa atagatccgt ccccggccgg
      301 tgcgttggcc ggtgtagact ggccgcgaaa accggccttt agggggaatt actggtgtcg
      361 tccgagccgt cgccggattg gcaagcgcca tccggcaaac gcatcgccct gagcgtcgcc
      421 gtcgcgatcg tcatcgcggc cgtggtgctc gtcgtggccg tgctgccggc cgagtacggc
      481 atcgatccga ccggcatcgg ccgcctgctc gggctcgacg agctcaaggc accctcgacg
      541 cgcacgatcg aggtcaagga cgtgctcggc ggcaacgagc gcgtgcgcga ggtcgagatc
      601 ccggcgttca acgagcccgt gccgctgccg aacccggccg tccatcaatc cgaggaacgg
      661 cccatccaaa cgcgcacgat cacgctgacg ctgaaggcgg gccaagagac cgagatcaag
      721 accgtgctgc gcacgaacaa ggtcatcgtc tacacgtggc actccgaggg cgggccggcc
      781 tattgcgact tgcacggcca cgacccggcc gccggcagcg agttcttcgt gcggtaccgc
      841 gaggaccagg agggcgcgac cgacgtgacc ggctcgctcg tcgcgccgtt cgacggcgag
      901 cacggttggt actggctcaa catcaacgag gtacccgtga cgattacgct caccgtcacg
      961 ggtttcttcg acgacatcgt cgactacggc attcactaac gagaacaaca ctgcatgtgg
     1021 caacagaact acgatcccct cggtaacttt ccactctcga cgctcgtggc tgcgctgccg
     1081 atattcgtgc tgctgctctt gatcggcatc ctgcgcaagc cggcgtggat gtcggcgctc
     1141 gcgggcttag gcactgcgct cgtcgtcgcg atcgtcgcgt acggcatgcc ggccaatctc
     1201 gccgtcagcg cgtcgttgat gggcgctgca ttcggcttgc tgccgatcgg ctggatcgtg
     1261 tactgggccg tcgtgctgta tcgcgtcacg ctcgagaccg gtaacttcga gatcatcaag
     1321 gactcgatcg gcggccttac cacggaccgc cgcctccagg cgatgctgat cgcgttcgcg
     1381 ttcggcgcgt tcgtcgaggg cgcggccggc ttcggcacgc cggtcgccgt cgcggccgcg
     1441 atgctgacgg ggctcggctt ctcgccgttc ttcgccgccg gcatttgcct gctcgcgaac
     1501 acggcgccgg tcgcgttcgg ctcgatcggc attcccgtca cgacgctcgc caatacgacc
     1561 ggcctacccg aggccgagct gtccgcctgg gtcggccgca tctgcgcgcc gatctcggtg
     1621 ttcattccgg cctacctcgt catggtcatg ggcgggctga aggggctcaa aggcgtgctg
     1681 ccggccgcga tcgtggtcgg cgtctgcttc gcgagcgtgc agctcacggt ctcgaacctg
     1741 atcggcccgc agctcgtcga catcttggcc gccttgacgg cgatcgtcgg cctcgtcgtg
     1801 ctgctcaagg tctggcagcc gaaggaccac ttcgtgctgg ccggcgagca cgccgccgtc
     1861 gcggtgccga agcactaccc gctcggccgg acgttgctgg cctggtcgcc ttacttgctg
     1921 ctggtcgcgt tcgttctggt ctggggctac ccggacacga aaacgatcct tgcgcgagcg
     1981 tcgattccca tcccgtggcc cggcctgcac aacgcgatca cgcgcatgcc gccggccgtc
     2041 gcggcgccgt caccgtacgg tgccgtctac gacttccaat ggctcgtggc cgcgggcacg
     2101 gcctgcgcgt ttgcgacgat ggcgtcggcg ctcgtgctgc gcgtctccgt ggccaaatac
     2161 ctcggcattc tcggccgcgt gttcaagcag ctcgcgttct cgctgctgac gatcgccgcc
     2221 gtgctcggcc tcgcgtacgt gatgaactac agcggcgcgg tcgtgacgct cgggctcgcg
     2281 tttgcagcga cgggcgcgct gttcccgttc ttcagctcgc tcgtcggttg gctcggcgtg
     2341 ttcctgacgg gcagcgacac gtcggcgaac gcactgttcg gcaatctaca ggtcgtcacg
     2401 gccaacagcc tcggcttcga cccggccgtc atggccgcat cgaactcggc gggcggcgtt
     2461 atgggcaaga tgatcagcct cacgagcctc gccgtcgccg tggcggcaac cggcatggcg
     2521 cggtcggagg aaggcaagct gttccgcttc accgtgaagc acagcgtgtt cctcgcctca
     2581 ctgctcggca tcatcgcgac gatctacgcg tacgcttacc gctgacgctc cgcagccgga
     2641 ggggacgata tcattcgggg tgagggtgag catgagagtg cgcccagtcc gcgaagcttg
     2701 ccacggtcgc caccagctcg tggggttcga tcggtttggc aatgtgcatc tgatagcccg
     2761 ccatcagtgc ccgagtgcga tcctctgatc gagcgaaagc ggtcagagcg atggccggca
     2821 ctcctccgcc gagctcggat gtacggcgcc tgacttgccg catgaactcg tacccgtcca
     2881 cgtcaggcat tccgatgtcg ctgatgatga catccgggcg tgacgagacg agagcttcga
     2941 gcgcttcctt cgcgctgccg acggtcctca cgtctgcgtg atactgctcc aagacgcgct
     3001 tgatgagctc gcgcgagtcg cgttcgtcct ccacgacgag cacgcgtaca ccctccaagt
     3061 cgacgttctc gagcttcgta atgaggctct tcgaggccgt agggtgttcc cactcctctt
     3121 tgctgcgcac gggagccagc ggcaaagtca cgacgaacgt tgcaccgcga ttctcgccgt
     3181 cgctgtgcgc ccgaacgctg ccgccgtgca actcgaccag gtgcttgacg atcgacaaac
     3241 cgagaccgag gccaccgtac gctcgcgagg gtgcggaatc agcttggcgg aaccgctcga
     3301 acacgtgcgg tagaaaatcc tggccaatac ccacgccgga gtctcggact gtgagctcga
     3361 cgtgcgaatt cactcgctga acgatcacgt cgacgttacc ggccttcggc gtaaatttca
     3421 cggcgttgga cagcaagttc cagacgatct gctgcaaccg gtttgggtcg ccggagaccg
     3481 gcccgatcga cggatccacg atctggcgaa ttctgattgc cttcgagtcc gcagataagc
     3541 gaatcgtctc gaccgcctgc tggacgacca tggggacatc ggtctcccgc acgtccaaac
     3601 gcaccttgcc ggaagcaatg cggctcatgt ccatcaagtc ttcgatcagc tgcgcttgcg
     3661 ctcgagcgtt tcgaacaatg gtctcgacgc cgcgcttcac atcctcgtcg ctgaccttgc
     3721 cggccgctat gacttgtgcc catcccagta ttgcgttgag cggcgtacgc agctcgtgag
     3781 aaacgctcgc aa
//
LOCUS       DASSNN010000829         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_736858, whole genome shotgun sequence.
ACCESSION   DASSNN010000829 DASSNN010000000
VERSION     DASSNN010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736858"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /gene="pssA"
                     /locus_tag="VFL84_15535"
     CDS             complement(<1..483)
                     /gene="pssA"
                     /locus_tag="VFL84_15535"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HET7610094.1"
                     /translation="MAKRFSMIREFHFADWFTLANAVSGMGAVLASMTYLETHDVLHV
                     YFACSAVLAALVFDVLDGRIARSRQTSSALGRELDSLADVISFGVAPAALAYACGMQG
                     FYDRVLLAYFVACGVSRLARYNVTADALSGGTGKVAYFEGTPIPTSILLVAMLAVAAY
                     R"
     gene            590..1186
                     /locus_tag="VFL84_15540"
     CDS             590..1186
                     /locus_tag="VFL84_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET7610095.1"
                     /translation="MAKRLEGKRIAFLAADYFEQIELEQPWNEIKDAGATVELLSVHQ
                     GSIQGMRHMDKGDTFEVDKLVSEADAEDYDGLVLPGGVANPDILRTNVPAVEFVRDFF
                     EQSKPVAAICHGPWTLVEADAVRGRTVTSWPSLKTDIRNAGGHWVDEEVHVEQGLVTS
                     RKPADLPAFCAKAIEEFAEGRHAGRRPGGARPSARPSA"
     gene            complement(1183..>2038)
                     /locus_tag="VFL84_15545"
     CDS             complement(1183..>2038)
                     /locus_tag="VFL84_15545"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7610096.1"
                     /translation="ALDYFTHVAPDSGEAALPDLWQNDTRVTTDGYLTDVFSERAAEY
                     VSRPRSKPFYLSLHYNAPHAPWEGPADRAIDHTDHGPGPMVSGGSLDAYAQMMRSMDE
                     GIGRVLAALRRARLERDTLVIFTSDNGGERYSFHWPFSFQKLYLFEGGIRVPAIVRWP
                     GVVPAGAVTEQAAITMDWTATILAAAGAQIDAARPLDGESLLPVLRGERAVYDRTLFW
                     RTRTRAAARIGRWKYVQEGDAEHLYDLGVDLGEKTDLKAREAAAFADAKSRYSAWAAG
                     MLPRPGSS"
BASE COUNT          362 a          696 c          687 g          293 t
ORIGIN      
        1 tcgataggcc gcgaccgcca gcatggcgac gagcaggatc gacgtcggga tgggcgtgcc
       61 ttcgaagtac gcgaccttgc ccgtgccgcc cgacagcgcg tccgccgtca cgttgtagcg
      121 cgcgagacgc gacacgccgc acgcgacgaa gtacgcgagc aacacgcgat cgtagaaccc
      181 ttgcatgccg cacgcgtacg cgagcgccgc cggcgcgact ccgaacgaga tcacgtccgc
      241 cagcgagtcg agctcgcggc cgagcgccga cgacgtctgc cgcgagcgcg cgatgcgtcc
      301 gtcgagcacg tcgaacacga gcgccgcgag cacggcgctg caggcgaaat agacgtgcag
      361 cacgtcgtgc gtttcgagat acgtcatgct cgcgagcacg gcgcccatcc ccgagaccgc
      421 gttcgcgagc gtgaaccaat ccgcgaaatg aaactcgcgg atcatcgaga atcgcttcgc
      481 catccgcaca cgctagcaca gcaccgcgaa tgccaggcac gcaacttgca gcctttcggc
      541 gcccgcggca ccacgccgca ccctcaacca cggacgatcg gagaccatca tggccaagcg
      601 actggaaggc aaacggattg catttctcgc agcggactac ttcgagcaaa tcgagctcga
      661 gcagccgtgg aacgaaatca aggacgccgg cgcaacggtc gagctgctct cggtgcacca
      721 aggctcgatt caaggcatgc gccacatgga caagggcgac actttcgaag tggacaagct
      781 cgtctcggaa gcggatgccg aggactacga cggcctcgtg ctgcccggcg gcgtcgcgaa
      841 cccggacatc ctgcgcacga atgtcccggc cgtcgagttc gtgcgcgact tcttcgagca
      901 gtcgaagccc gtcgccgcga tttgccacgg cccgtggacg ctcgtcgagg ccgacgccgt
      961 gcgcggccgc accgtgacgt cgtggccgag cttgaagacg gacatcagaa atgcgggcgg
     1021 ccattgggtg gacgaggaag tccacgtcga gcaagggctc gtgacgagcc gcaagccggc
     1081 cgatctaccg gcgttctgtg ccaaggcgat cgaagagttc gcggaaggcc ggcacgcggg
     1141 ccggcgcccg ggcggcgcac ggccgtcggc gcggccttcg gcttaagagc tcccgggccg
     1201 cggcagcatc ccggccgccc acgcggaata gcggctcttc gcatcggcga aggccgcggc
     1261 ctcgcgggcc ttcaagtccg tcttctcgcc gaggtcgacg ccgagatcgt agagatgctc
     1321 ggcgtcgcct tcctgcacgt atttccagcg gccgatgcga gcagccgcgc gcgttcgcgt
     1381 gcgccagaag agcgtgcggt cgtagacggc gcgctcgccg cgcagcacgg gcaacaggct
     1441 ttcgccgtcg agcggtcgcg ccgcatcgat ttgcgcgccc gccgcggcga ggatcgtggc
     1501 ggtccagtcc atcgtgatcg cggcttgctc ggtcaccgcg ccggccggca cgacacccgg
     1561 ccagcgcacg atcgcgggca cgcggatgcc gccttcgaag agatagagct tctggaacga
     1621 gaacggccaa tggaacgagt aacgctcgcc gccgttgtcg ctcgtgaaga tcacgagcgt
     1681 gtcgcgctcg agccgcgcgc ggcgcagcgc ggcgagcaca cggccgatgc cttcgtccat
     1741 gctgcgcatc atctgcgcgt acgcgtcgag cgacccgccg ctcaccatcg gtcccggccc
     1801 gtgatcggtg tgatcgatcg cgcggtccgc cggcccttcc catggcgcgt gcggcgcgtt
     1861 gtagtgcagg ctcagataaa aaggtttgct gcgggggcgc gaaacgtatt cggcggcgcg
     1921 ttcgctgaag acgtccgtca gataaccgtc cgtcgtgacg cgcgtgtcgt tctgccaaag
     1981 atccggcagc gccgcctcgc cggagtccgg cgcgacgtgc gtgaagtaat cgagcgcg
//
LOCUS       DASSNN010000830         3553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1372606, whole genome shotgun sequence.
ACCESSION   DASSNN010000830 DASSNN010000000
VERSION     DASSNN010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372606"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /gene="ampD"
                     /locus_tag="VFL84_15550"
     CDS             complement(<1..512)
                     /gene="ampD"
                     /locus_tag="VFL84_15550"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HET7610097.1"
                     /translation="MIVDRATGWLVGVRRVLSPNHDERPAGSSLSLIVVHGISLPPGE
                     FGNGWIDRFFCNDLPADAHPYFATIQHLTVSSHVLIARDGTPTQYVPFDRRAWHAGKS
                     EYCGQSACNDFSVGIELEGTDDLPYDAAQYRALAELIVALRRGYPTLRNAEVVGHSDI
                     APGRKTDPGPA"
     gene            608..1357
                     /locus_tag="VFL84_15555"
     CDS             608..1357
                     /locus_tag="VFL84_15555"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET7610098.1"
                     /translation="MADTPHRTPRLYVELELDGASVALDESEAHYLGHVLRLAPGRRL
                     VVFNGRGTEREASVDSLQRRGAVLALAAVRRPLPESPLDLTLVQALPKSDAMDLIVQK
                     ATELGVRSLWPVHTEFSVVKLDAERSERRLDHWRKIARSACEQCGRHTPPRIERPRQL
                     AAALDALPAATKRLALEPSAERSLGEQPPAHEGLVVAVGPEGGFGAADWRRLDAAQFT
                     RVSLGPRILRAETAALAACAIAQSRWGDLRG"
     gene            complement(1370..1840)
                     /locus_tag="VFL84_15560"
     CDS             complement(1370..1840)
                     /locus_tag="VFL84_15560"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7610099.1"
                     /translation="MSADPKELYSLLIPLRSERLLVPRMCVAEVIAFVDAARSRDDAA
                     PDWFLGAIEWNNRRLPVVSFDSQRGDERKARRARTRVVVFHAITQELKGGHYGILTQG
                     FPQLVRVNADVLGLDTEQPLPEGLPALCRARMIHEYPLIPDVERLEKMIAAIPL"
     gene            complement(1842..>3553)
                     /locus_tag="VFL84_15565"
     CDS             complement(1842..>3553)
                     /locus_tag="VFL84_15565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7610100.1"
                     /translation="HSIEFHLGELGGTVTRLREQLRKLEAETEAQILHRHQDDADVES
                     GFDPLELDRYSTIQQLSRALAESANDVASINELLHGLTNEADTLLTQQARVTSDLQTG
                     LMQTRMVPFQRHVSRLQRIVRQACADTGKQAELVVDGESNEIDRQVLESMLPPFEHLL
                     RNAIAHGIEKPAVRHKRGKPEAGKVLLKVRREGAEVIVEVADDGGGLDLAAIRRKAYE
                     KGLLAENQRITDEQAVELILQPGFSTASELTQAAGRGVGMDVVDNEIKKLGGSMRIET
                     RTGEGTRFLIRLPYTLAITHALIVNVGDETFALPLPTVEGITRLSRDKILKHLTEDEP
                     KLDYGGIVYRIQHLGSLVGAAPSALPEDENAVSLVLIRAGDNSTALLMDSLEGSREIV
                     VKTLGPHIASVPGVTGATILGDGRVIMILDPGTLVRAHRPLQPVAPAPARSSAAQLTA
                     LVVDDSITMRRVTQRLLERRGAKVFTARDGLDAITVLQEHTVDVILLDIEMPRMDGYQ
                     FATHVRNDPKQKGVPIIMITSRSGEKHRAKAIEIGVNDYLSKPYQESQLVAAIEALVG
                     RSL"
BASE COUNT          541 a         1215 c         1204 g          593 t
ORIGIN      
        1 gccggcccgg gatcggtctt gcggccgggg gcgatgtcgc tgtgcccgac cacctcggcg
       61 ttacgcagcg tcgggtagcc gcgccgcagc gccacgatca gctcggcgag cgcgcggtac
      121 tgcgccgcgt cgtacggcag atcgtccgtg ccctcgagct cgatcccgac cgaaaagtcg
      181 ttgcacgcgg actgtccgca atattccgac ttgccggcgt gccatgctcg gcggtcgaac
      241 ggcacatact gcgtcggcgt cccgtcgcgt gcgatcagca cgtgagacga gaccgtcaga
      301 tgctggatcg tcgcgaaata cggatgcgcg tcggccggca gatcgttgca gaagaagcgg
      361 tcgatccagc cgttgccgaa ctcgccgggc ggcaagctga tgccgtgcac gacgatgagg
      421 ctcaagctcg agccggcggg ccgctcatcg tggttcggcg acaagacgcg gcgcacgccg
      481 accagccacc ctgtcgcgcg atcgacgatc acgctggttc gagcaacttg ctaaagtgcg
      541 ccgcagacat gcgcgccaaa ctatagcagc ctcatcgcac gccgccgcat cgagctccaa
      601 gccccccatg gccgacacgc cgcaccgaac gccgcgcctc tatgtcgagc tcgagctcga
      661 cggcgcgagc gtcgccctcg acgagagcga ggcacattat ctcggtcacg tcctgcggct
      721 tgcgcctggg cgtcggctcg tcgtgttcaa cggccgcggc acggagcgcg aagcgagcgt
      781 cgactcgctg cagcgccgcg gtgccgtgct cgctctcgcg gccgtacgcc gtccgctgcc
      841 ggaatcgccg ctcgatctga cgctcgtgca agcgctgccg aaatccgacg cgatggacct
      901 catcgtgcag aaggcgaccg agctcggcgt gcgctcgctc tggccggtcc atacggaatt
      961 cagtgtcgtg aagctcgacg ccgaacgcag cgagcggcgc ctcgaccact ggcgcaaaat
     1021 cgcgcgaagc gcgtgcgagc aatgtggccg gcacactcca ccgcgcatcg agcggccgcg
     1081 gcagctcgcc gccgcgctcg acgcactgcc ggcggcgacg aagcgcctcg cgctcgagcc
     1141 ctcggcggag cggagtctcg gcgagcagcc gcccgcgcac gaagggttgg tcgtggccgt
     1201 cgggcccgag ggcggtttcg gcgcggcgga ttggcggcgg ctcgacgcgg cgcaattcac
     1261 gcgcgtaagc ctcggtccgc gcatcctgcg cgccgaaacg gcagcgctcg cggcctgtgc
     1321 gatcgcgcag tccaggtggg gcgacttgcg gggttgaacg gccgggtccc tagagcggga
     1381 tcgccgcgat catcttctcg agccgctcga cgtcggggat cagcggatac tcgtggatca
     1441 tccgagcacg gcacagcgcg ggcagacctt ccggcagcgg ctgctccgta tcgagcccga
     1501 gcacgtcggc gttgacgcgc acgagctgcg ggaacccttg agtcaagatc ccgtagtggc
     1561 cgcccttgag ctcttgcgtg atcgcgtgaa agacgacgac acgtgtgcgc gcgcgcctcg
     1621 ccttgcgctc gtcgccgcgt tggctgtcga acgacacgac gggcaggcgc cggttgttcc
     1681 actcgattgc gccgagaaac caatcgggcg ccgcgtcgtc gcggctccgc gccgcatcga
     1741 cgaacgcgat gacttccgcc acacacatgc gcggaacgag cagccgctcg ctccggagcg
     1801 gaatcagcaa gctgtacagc tctttgggat cggcgctcac gtcacaggct ccgaccgacc
     1861 agcgcctcga ttgcggcgac gagctggctc tcttgatacg gcttgctcag gtagtcgttg
     1921 acgccgatct cgatggcctt cgcgcggtgc ttctcgcccg agcgcgacgt gatcatgatg
     1981 atcgggacgc ctttctgctt cggatcgttg cgcacgtgtg tcgcgaattg atagccgtcc
     2041 atgcgcggca tctcgatgtc gagcaggatg acgtcgacgg tgtgctcttg cagcaccgtg
     2101 attgcatcga gaccgtcccg agccgtgaag accttcgcgc cgcgccgctc gagcagccgc
     2161 tgcgtcacgc gccgcatcgt gatcgaatcg tccacgacga gcgccgtcag ctgcgccgcg
     2221 ctgctccggg cgggcgcggg cgcgaccggc tgcagcggcc gatgcgcgcg cacgagcgtg
     2281 cccgggtcga ggatcatgat gactcggccg tcgcccaaga tcgtggcgcc cgtgacaccc
     2341 ggcacgctcg cgatgtgcgg accgagcgtc ttgacgacga tctcgcggct gccttcgagc
     2401 gaatccatca ggagcgcggt cgagttgtcg ccggcgcgaa tgagcacgag cgagacggcg
     2461 ttctcgtctt cgggcagcgc cgacggggcc gcgccgacga ggctgcccaa gtgctggatc
     2521 cgatagacga ttccgccgta gtcgagcttc ggctcgtcct cggtgaggtg cttcaggatc
     2581 ttgtcgcgcg agaggcgcgt gatgccctcc acggtcggca gcggaagcgc gaaggtctcg
     2641 tcgccgacgt tcacgatcaa cgcatgcgtg atcgcgagcg tgtacggcag gcggatcagg
     2701 aaccgcgtgc cttcgcccgt gcgcgtttcg atgcgcatcg agccgccgag cttcttgatc
     2761 tcgttgtcga cgacgtccat gccgacgcct cgaccggccg cctgcgtcag ctcgctggcc
     2821 gtgctgaagc ccggctgcaa gatcagctcg acggcctgct cgtcggtgat ccgctgattc
     2881 tcggcgagca gccccttctc gtaggctttg cgccggatcg cggcgaggtc caggccgccg
     2941 ccgtcgtcgg caacctcgac gatgacctcg gcgccttcgc ggcgaacctt gagcaggacc
     3001 ttgccggcct cgggcttgcc gcgcttgtgc cgcacggccg gcttctcgat gccgtgcgcg
     3061 atcgcattgc gcagcaggtg ctcgaacggc ggcagcatcg actcgagcac ttggcggtcg
     3121 atctcgttcg actctccgtc gacgacgagc tcggcttgct tgccggtgtc ggcgcaggcc
     3181 tgccgcacga tgcgctgcaa gcgcgacacg tgccgttgga acggcaccat gcgcgtctgc
     3241 atgaggcccg tctgcaaatc ggacgtcacg cgcgcctgct gcgtcaacag cgtgtcggcc
     3301 tcgttcgtga gaccgtgcag cagctcgttg atgctcgcca cgtcgttcgc cgattcggcg
     3361 agcgcgcgag agagctgctg gatcgtcgaa taacgatcga gctcgagcgg atcgaagccg
     3421 ctttcgacgt cggcatcgtc ctggtgccga tgcaggatct gcgcctcggt ctcggcttcg
     3481 agcttgcgca gctgctcgcg caggcgcgtc accgtgccgc cgagctcacc caagtggaat
     3541 tcgatcgagt gca
//
LOCUS       DASSNN010000831         3039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1753784, whole genome shotgun sequence.
ACCESSION   DASSNN010000831 DASSNN010000000
VERSION     DASSNN010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753784"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="VFL84_15570"
     CDS             complement(<1..1267)
                     /locus_tag="VFL84_15570"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7610101.1"
                     /translation="MNATRWNRREVLGGVLAAGALSGAPFRPGFAQQPAGTMSERREL
                     LIRNAYVLTMDAAVGDIAGGDVHLRGGAIAAVGRGLDAAGAEIVDGTGMLVLPGFVET
                     HWHIWTALLRSLAGDRQEHGYFPTSRTVGTFYSAEDMYAAGRLAAAEAINAGITFVHD
                     WCHNVRGPDYAEAALRALAETGIRARFSYGSPTAASNDATIDLKDLRRLSERWRDHAN
                     GGLLTLGLAGRGAASAATLQDYELAKELGLPISVHANNFESSAGGIQQLADRGLLAPG
                     MQVIHAVWCTPAEIRALAENRVNVSVSPYSELRIGFGMPIAAKLVAAGVTVGLSVDTT
                     TLSGNADMFAIMKAIQNIENGRAKNEFELTARRVLELATIDGARSMGIGDSVGSLTPG
                     KRADVIMVDTRALNLAVLTEPAHLLVEAAQ"
     gene            complement(1264..1980)
                     /locus_tag="VFL84_15575"
     CDS             complement(1264..1980)
                     /locus_tag="VFL84_15575"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7610102.1"
                     /translation="MSSVIDPSGKEAIRRREAAERPAGLGGVASLVRVLDESRLKPKA
                     KEQEVLDVATEYFLQHGYRGASINAMARSSGISKESIYRYFSSKQQLFEAVIGRELIE
                     YRRNLHRLDATLRSMDLRAALITVSEAILAIITTDRTMALRRLIFDEATRSPEVGQHY
                     YKIGAAQAYTVLESVLGTHAHESDFDVPTLARHFAAMLCWRMTLERQCAVRAQPGQEE
                     IAQLSAAVVDDFMKAFLKRK"
     gene            complement(2004..>3039)
                     /locus_tag="VFL84_15580"
     CDS             complement(2004..>3039)
                     /locus_tag="VFL84_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7610103.1"
                     /translation="NAYTRRSPVGVVGQIIPWNFPLAMAAWKLGPALAAGCTCILKPA
                     EQTPLTALRLGDLIIEAGFPPGVVNILTGYGENVGAALVTHPGVDKIAFTGSTLVGKA
                     INKAATDTLKRVSLELGGKSPVIVLPDADIETVVGGAAGAIFFNAGQVCAAGSRLYVH
                     RKIFDRVVEGVGGAAQAIKLGPGLDPTTQMGPLVSREQQERVMGYIKSGRDEGASVVA
                     GGDTPSHRGFYVKPTVMADVNRSMRVVQEEIFGPVLVAQRFDDLDEVAALANDTPYGL
                     SASIWSNDLTAVHRLVPKIKAGTVWVNTHGPVDANMPFGGYKHSGLGREHGRVGVEMY
                     TELKSVCMLV"
BASE COUNT          476 a         1029 c         1011 g          523 t
ORIGIN      
        1 gctgcgcggc ttcgacgagc agatgcgcgg gctccgtgag cacggcgaga ttcagcgcgc
       61 gcgtgtcgac catgatgacg tccgctcgct tgccgggcgt caacgagccg acgctgtcgc
      121 cgatgcccat cgaacgcgca ccgtcgatcg tcgcgagctc gagcacgcgc cgcgccgtga
      181 gctcgaactc gttcttggcg cggccgttct cgatgttctg gatcgccttc atgatcgcga
      241 acatgtcggc attgcccgaa agcgtcgtcg tatcgacgga caggccgacc gtgacgcccg
      301 cagcgacgag cttggcggcg atcggcatgc cgaagccgat gcgcagctcg gaatacggcg
      361 acacgctgac gttgacgcga ttttcggcca gcgcgcgaat ctccgcgggc gtgcaccaga
      421 ccgcgtgaat gacttgcatg ccgggagcga gcaagccgcg gtccgcgagc tgctgaatgc
      481 cgccggcgct gctctcgaag ttgttcgcgt gcaccgagat cggcaggccg agctccttcg
      541 cgagctcgta gtcttgcagc gtcgccgccg atgccgcgcc gcgccccgcg agcccgagcg
      601 tcaacaaccc gccgttcgcg tggtcgcgcc accgttccga cagccttctc aagtctttga
      661 gatcgatcgt cgcgtcgttg ctggccgcgg taggcgagcc gtaggaaaag cgcgcgcgta
      721 tgccggtctc cgcgagcgcg cgcaacgccg cctcggcata gtcggggccg cgcacgttgt
      781 ggcaccagtc gtgcacgaac gtgatgcccg cgttgatcgc ttcggccgcg gcgagtctgc
      841 ctgccgcgta catgtcctcg gcggaataga acgtgccgac ggtgcgcgac gtcgggaaat
      901 agccgtgctc ttggcgatcg ccggcgaggc tgcgcagcaa cgcggtccag atgtgccagt
      961 gcgtctcgac gaatcccggc agcacgagca tgccggtgcc gtcgacgatc tcggcgcccg
     1021 cggcgtcgag gccgcggccg accgctgcga tcgcgccgcc gcgaaggtgc acgtcgccgc
     1081 ccgcgatgtc gccaacggcc gcgtccatcg tcaggacgta ggcattacgg atcaggagct
     1141 ctctacgctc gctcatcgta cccgccggct gctgcgcaaa gcccggccgg aacggcgcac
     1201 cgctcaaggc gcccgcggcg agcacgccac cgagcacctc gcgccggttc caccgcgtgg
     1261 cgttcacttc cgcttgagga atgccttcat gaaatcgtcg acgacggcgg ccgacaattg
     1321 cgcgatctct tcctgcccgg gctgcgcgcg cacggcgcat tgccgctcga gcgtcatccg
     1381 ccagcacaac atcgcggcga agtgccgcgc gagcgtcgga acgtcgaagt cgctctcgtg
     1441 cgcatgtgtt ccaaggacgc tctcgaggac tgtgtacgct tgggcggctc cgatcttgta
     1501 gtagtgctgg cccacttccg gcgagcgcgt cgcctcgtcg aagatcagcc ggcgcagcgc
     1561 catcgtgcgg tccgtcgtga tgatcgcgag gatcgcctcg gacaccgtga tcaacgccgc
     1621 ccgcaagtcc atgctgcgca gcgtcgcgtc gagccggtgc agattgcgcc gatactcgat
     1681 gagctcgcgg ccgatgaccg cctcgaagag ctgctgcttg ctgctgaaat agcggtagat
     1741 cgattccttc gagatgccgg agctgcgcgc catcgcgttg atgctcgcgc cgcggtatcc
     1801 gtgctgcagg aagtactcag ttgcgacgtc gagcacctct tgctctttgg ccttcggctt
     1861 caggcggctt tcgtctagca ctcgcacgag gctcgcgacg ccgcccaaac cggcggggcg
     1921 ctccgccgcc tcgcggcggc gaatagcttc cttgccgctg ggatcgatta ctgaactcat
     1981 cgtgactcct gctccatcta ttcttagacg agcatgcaca cggacttcag ctccgtgtac
     2041 atctcgacgc cgacacggcc gtgctcgcgg ccgagcccgg aatgcttgta gccgccgaac
     2101 ggcatgttcg cgtcgacggg gccgtgcgtg ttgacccata ccgtgccggc tttgattttc
     2161 ggcaccagcc ggtgcacggc ggtgagatcg ttcgaccaga tgctcgcgct gagcccgtag
     2221 ggcgtgtcgt tggcgagcgc cgcaacctcg tcgaggtcgt cgaaacgctg cgctacgagc
     2281 acggggccga agatttcctc ctgcacgacg cgcatcgaac gattgacgtc cgccatgacg
     2341 gtcggcttca cgtagaagcc gcggtgcgac ggcgtatcgc cgccggcgac gacggaggcg
     2401 ccttcgtcgc ggccggactt gatgtagccc atgacgcgct cttgctgctc gcgcgacacg
     2461 agcgggccca tctgcgtcgt gggatcgagg cccggtccga gcttgatcgc ttgcgcggca
     2521 ccgccgacgc cttcgacgac gcgatcgaaa atcttacggt gcacgtacag gcgcgagccc
     2581 gcggcgcaca cttgccccgc gttgaagaag atcgcgccgg ccgcgccgcc gacgaccgtc
     2641 tcgatgtccg catcgggcag cacgatgacc ggggacttgc cgccgagctc gagagacacg
     2701 cgcttcaacg tgtccgtggc cgctttgttg atggctttgc cgacgagcgt cgagcccgtg
     2761 aacgcaatct tgtcgacgcc gggatgcgtc acgagcgcgg cgccgacgtt ctcgccgtag
     2821 ccggtgagaa tgttcacgac gccgggcgga aagccggcct cgatgatcag atcgccgagc
     2881 cgcagcgcgg tgagaggagt ctgctcggcg ggcttcagga tgcacgtgca gcccgcggcg
     2941 agtgcgggac cgagcttcca tgcggccatc gcgagcggga agttccacgg aatgatttgc
     3001 ccgacgacgc cgaccggcga acggcgtgtg tacgcgttc
//
LOCUS       DASSNN010000832         3804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1374036, whole genome shotgun sequence.
ACCESSION   DASSNN010000832 DASSNN010000000
VERSION     DASSNN010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374036"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VFL84_15585"
     CDS             <1..704
                     /locus_tag="VFL84_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610104.1"
                     /translation="VTVSGAVERNVDGFGQTVSVESTARVGGNVTGHVDSAGDLSVAD
                     GATVGGTVDEQLVEREQRRNHYETFGYYFGQIIRLAGAFLTGLALLWLFPALREASLP
                     NVVAVLRSTGIGLAAAVTLPVAALIVCVTIVGIPLGILTFVLGAIGLYFSKAVIAQII
                     GRAVLRNRPTPPHFAVTLFVGLVIVIVAINLPGIGGLANLLLTLTGFGVIVTLIFARL
                     NRAEPVPPSRALELR"
     gene            747..1748
                     /locus_tag="VFL84_15590"
     CDS             747..1748
                     /locus_tag="VFL84_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HET7610105.1"
                     /translation="MLRSIATVFALAAALSAAGESLNHPTGTQGVLLVDKLGGYVRFL
                     DAGTLRERSRLELPKNPHDFALSADHRLAYVPIYGPGIYGRNPEPGHELYVLDLAEQK
                     VASVIDLAPYRSPHGVQIDANGMLYLTAELDRKILVVDPKAGRIVDTIAHEGSGHWVA
                     VLPDGSKAYVANKNDKPFLSVLDLKARKQIGTVPMPRGTQGVTASPDGKRIIAMDLAR
                     PELAVIDTATDTVLERVALAGTTDPAYKAYYSPDGKWLLTMAGSSISIFDAANLLAPQ
                     RTLKVGASPMGIGFSADGKTALVANHGDGTVSAVDIASATVTKTFKAGTGIETLTYY"
     gene            complement(1755..2441)
                     /gene="lepB"
                     /locus_tag="VFL84_15595"
     CDS             complement(1755..2441)
                     /gene="lepB"
                     /locus_tag="VFL84_15595"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009573936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7610106.1"
                     /translation="MPPTDSKPSVTSSGTGAKPGRAKWWISELTSLAIVLAAVTAARS
                     SLADHYYVPSGSMQYTLMDGDRVVVNKMAYGFRIPLTKIDIFGSSTPTRGDIAVFDSP
                     EEGHVRLIKRIVAIGGDHVSVRNGQLSINGQPLGDRKVEHFGDREALLNLTSGGGDDY
                     EMDIPPGMVLAMGDNRGNSNDGRKFGLIDERELFGRAVAIYYRKADGALKCAKHLGCP
                     AGSGFTWLPL"
     gene            complement(2483..3727)
                     /locus_tag="VFL84_15600"
     CDS             complement(2483..3727)
                     /locus_tag="VFL84_15600"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET7610107.1"
                     /translation="MSRFHAVVSIVAAAVAGNAFAHHGIANFDLNKDISISGSVSKLA
                     FVNPHSWLYVSVVGADGKTTEWQCEMRAATVLRRSGWSEAMFTAGTAVTITGSPDRQQ
                     PNTCYLGTITFADGTSMDRYGQRQKSTAAVAADRPERLPNGDPNLNGDWAGEQRVMTD
                     RRGISGALVPISVADRLAPGAVPEGGRAFPGARGTPESLADNAIRAAWTRPSPVPLTE
                     AGRRAAEGFDPSSTDNPRLNCEPTNILFDWGFETFTNRITQDENSIRIQYGAPGIDRR
                     IDLNETAHPAGIEPSVTGHSIGHFENDVLIVDTVGFKPGVLSADGYTMHSGELHVVER
                     FSLDRSSGALALRREYTAEDPLYFTGQFRGADVAYVADLPYETPTCTDLSYKTGVGTP
                     ATADAAAKPPAAPSKPWWMFWK"
BASE COUNT          596 a         1288 c         1275 g          645 t
ORIGIN      
        1 aggtcaccgt cagcggcgcc gtcgagcgca acgtcgacgg gttcggccag acggtgagcg
       61 tcgagtcgac ggcacgcgtc ggcggcaacg tcacgggtca cgtcgacagc gccggcgact
      121 tgagcgtcgc agacggcgcc acggtcggcg gcaccgtcga cgagcagctc gtcgagcgcg
      181 agcagcgccg caatcactac gagacgttcg gctactactt cggccagatc atccggctcg
      241 ccggcgcgtt cctgaccggc ctggcattgc tgtggctctt ccccgctctg cgtgaagcgt
      301 cgctgccgaa cgtggttgcc gtcctgcgct cgaccggcat cgggctcgcg gccgcggtca
      361 cgctgcccgt cgccgcgctg atcgtgtgcg tgacgatcgt cggcattccg ctcggaatcc
      421 tgacgttcgt gctcggcgcg atcggcctct acttctcgaa ggccgtgatc gcgcagatca
      481 tcggtcgcgc cgtcctgcgc aaccggccga cgccgccgca ctttgccgtg acgctgttcg
      541 tcggtctcgt catcgtcatc gtggcgatca atttgcccgg gatcggcggg ctcgcgaacc
      601 tgcttctgac gctgaccggg ttcggcgtga tcgtgaccct gatcttcgcg cgcttgaacc
      661 gcgccgagcc cgtgccgccg tcccgagcgc ttgagctacg ataacgacag cgctgcgcgg
      721 cgtttcgccg cggccgggga gacacgatgc ttcgatcgat cgcaaccgtt ttcgcgctcg
      781 cggccgcgct gtccgccgcc ggcgagagcc tgaaccaccc gacgggcacg caaggcgtgc
      841 tgctcgtcga caagctcggc ggctacgtgc ggttcctcga cgccggcacg ctgcgcgagc
      901 gctcgcgcct cgagctgccg aagaatccgc acgacttcgc gttgtcggcc gaccatcggc
      961 tcgcgtacgt gccaatctac gggccgggca tctacggccg caatccggag ccgggccacg
     1021 agctctacgt gctcgacctg gccgagcaaa aagtcgcgag cgtcatcgac ctcgcgccgt
     1081 atcgctcacc gcacggcgtg caaatcgacg cgaacggcat gctgtacctg acggcggagc
     1141 tcgaccgcaa gatcctcgtc gtcgatccca aggccgggcg catcgtcgac acgatcgctc
     1201 acgagggctc gggccattgg gtcgcggtgc tgcccgacgg cagcaaagcc tacgtcgcga
     1261 acaagaacga caagccgttc ttgagcgtgc tcgatctcaa ggcacgcaag cagatcggaa
     1321 ccgtgccgat gccgcgcggc acgcaaggcg tgaccgcatc gccggacggc aagcgcatca
     1381 tcgccatgga cttggcgcgg ccggagctcg ccgtcatcga cacggccacg gatacggtgc
     1441 tcgagcgcgt cgcgcttgcc ggcacgacgg acccggccta caaggcgtac tactcgcccg
     1501 acggcaaatg gctcttgacg atggccggct cgtcgatctc gatcttcgac gccgcgaacc
     1561 tgctcgcgcc gcaacgcacg ctgaaagtcg gcgcgtcacc gatgggcatt gggttctcgg
     1621 cggacggcaa gacggcgctc gtcgcgaatc acggcgacgg caccgtgtcg gccgtcgaca
     1681 tcgcgtcggc gaccgtcacg aagacgttca aagccggcac cgggatcgag acgctgacct
     1741 actactgacc gcggttacag cggcagccag gtgaatcccg agccggccgg gcatcccaag
     1801 tgcttggcgc acttcaacgc gccgtccgct ttgcggtagt agatggcgac ggcgcggccg
     1861 aacagctcgc gctcgtcgat gaggccgaac ttgcggccgt cgttgctgtt gccgcggttg
     1921 tcgcccatcg cgagcaccat gcccggcgga atgtccatct cgtagtcgtc gccgccgccg
     1981 ctcgtcaggt tcaacaatgc ctcgcggtcg ccgaagtgct cgacctttct gtcgccgagc
     2041 ggctgaccgt tgatcgagag ttgcccgttg cgcaccgaga cgtggtcgcc gccgatcgcg
     2101 acgatgcgct tgatgagccg cacgtggccc tcctcgggcg agtcgaacac ggcgatgtcg
     2161 ccgcgcgtcg gcgtcgacga gccgaagatg tcgatcttcg tgagcgggat gcgaaaaccg
     2221 tacgccatct tgttgacgac gacgcggtcg ccgtccatca acgtgtattg catcgagccg
     2281 gacggcacgt agtagtgatc cgcgagcgac gagcgtgcgg cagtgacggc cgcgagcacg
     2341 atcgcgagcg acgtcagctc cgagatccac catttggcgc ggcccggctt cgcgccggta
     2401 ccggaagacg taacgctggg tttgctatct gtagggggca cggagactcc gactgtttaa
     2461 gttcgaacgg gttccggaag cgtcacttcc agaacatcca ccaaggtttc gacggggccg
     2521 cgggcggctt ggcggcggca tccgccgtcg cgggcgttcc gacgccggtc ttgtacgaga
     2581 gatcggtgca ggtcggcgtc tcgtacggca aatcggcgac gtaggcgaca tccgcgccgc
     2641 ggaattggcc cgtgaagtac agcggatctt cggccgtgta ctcgcgccgc agggcgagcg
     2701 cgccgctcga gcgatcgagc gagaagcgct cgacgacgtg cagctcgccg ctgtgcatcg
     2761 tatagccgtc ggccgacagc acgccgggct tgaagccgac cgtgtcgacg atcagcacgt
     2821 cgttctcgaa gtgcccgatc gagtggcccg taacgctcgg ctcgatgccg gccggatgcg
     2881 ccgtctcgtt cagatcgatc ctgcgatcga tcccgggcgc gccgtattga atgcggatcg
     2941 aattctcgtc ctgcgtgatg cggttcgtga acgtctcgaa gccccaatcg aacaggatgt
     3001 tggtcggctc gcagttgagg cgcggattgt ccgtcgacga cgggtcgaag ccttcggcgg
     3061 cgcggcgccc ggcttcggtc aacggaaccg ggctcggcct tgtccacgcc gctcgaatcg
     3121 cgttgtccgc cagcgactcg ggcgtgccgc gcgcgcccgg gaacgcgcgc ccgccttcgg
     3181 gcaccgcgcc gggcgcgagc cggtcggcga cgctgatcgg cacgagcgcg ccgctgatgc
     3241 cgcggcggtc cgtcatgacg cgctgctcgc cggcccagtc gccgttcaaa ttcggatcgc
     3301 cgttcggcag gcgctcgggg cgatccgccg cgacggccgc cgtggatttt tgccgttggc
     3361 cgtagcggtc catgctcgtg ccgtcggcga acgtgatcgt gcccaagtag cacgtgttcg
     3421 gctgctgccg gtctggcgag cccgtgatcg tgaccgcggt gccggccgtg aacatcgcct
     3481 cgctccaccc cgagcggcgc agcaccgtcg cggcgcgcat ctcgcactgc cattcggtcg
     3541 tcttgccgtc ggcgccgacg acgctgacgt agagccacga gtgcggattc acgaacgcga
     3601 gcttcgagac cgagccgctg atcgagatgt ccttgttgag atcgaaattc gcgatgccgt
     3661 ggtgcgcgaa cgcattcccg gctacggcgg cggctacgat cgacacgacg gcgtgaaagc
     3721 gagacatggc tactcttgcg gccagtgcag ctttgcactg tgacattgaa ggccctttct
     3781 cggcaaatcc ggcgcggaaa ccgg
//
LOCUS       DASSNN010000833         4614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1754318, whole genome shotgun sequence.
ACCESSION   DASSNN010000833 DASSNN010000000
VERSION     DASSNN010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754318"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /gene="leuD"
                     /locus_tag="VFL84_15605"
     CDS             <1..567
                     /gene="leuD"
                     /locus_tag="VFL84_15605"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET7610108.1"
                     /translation="PIKEIRSRTVVLPNVDVDTDQIIPARFLTTTTKQGLGKNLFNDW
                     RYGKDGRELPDFVLNRPENRGATVLVAGNNFGCGSSREHAPWALLDFGIRAVVSTEIA
                     DIFRSNSLKNGLLPVIVDASTHRWLLDNPGADVRIDLETTTLEANGKRVPFPIDRFSR
                     YCLMNGIDQLGFLLEQRDAIARYERAHA"
     gene            564..1634
                     /gene="leuB"
                     /locus_tag="VFL84_15610"
     CDS             564..1634
                     /gene="leuB"
                     /locus_tag="VFL84_15610"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET7610109.1"
                     /translation="MSSKIVVLAGDGVGPEVTAAAVRVLEQVSVRHSLKLEFEDALIG
                     GAAYDAAGLPLPDATVKACNASTAVLLGAVGGPKWADIPVQHRPEQGLLAIRKALGLF
                     ANLRPVTTTPELAAASPLKPEKLAGVDILVIRELTGGIYFGKRSRTADSAFDTCEYTA
                     AEIERVCRVAGKLAMARRRKVTSVDKANVLDTSRLWRDVATRVFKNEFPELTVEHLLV
                     DAAAMHLLSRPADFDVMVTENMFGDILTDEASMLAGSMGLLPSASLGDGGPGLYEPIH
                     GSAPDIAGQGVANPCGTILSAAMLLRHSVGAEAAAKAVEAAVAGALRSGARTRDIAGP
                     GARNWLGTAAFTDAVLKHLAAP"
     gene            1723..2028
                     /locus_tag="VFL84_15615"
     CDS             1723..2028
                     /locus_tag="VFL84_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HET7610110.1"
                     /translation="MKTNRRSARDTQRAEQGLYRAILSLRNEAECEQFFADLCTPAEL
                     EALVDRWAVVKYLQDGLPYRKIHELTGVSVTTIGRVARFLIAGNGGYKTALKRLSPL"
     gene            2050..2949
                     /gene="hisG"
                     /locus_tag="VFL84_15620"
     CDS             2050..2949
                     /gene="hisG"
                     /locus_tag="VFL84_15620"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013746535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET7610111.1"
                     /translation="MTTDSRIKIAVQKSGRLTEHSLELLVRCGLSYSRGKDQLICYGE
                     NMPVDVLLVRDDDIPRLVQEDICDLGIVGLNIIEERRLELKEAGETELFTEIGRLDFG
                     QCRLSFGLPAGVQYGGPKQLQGKRIATTYPRILRDYLKKHGVEAQIVAFSGAVEIAPS
                     LGKADLICDLVSSGATLRAHQLYEAATILESRAALIQTPKPLTGDKAEWLQRLLLRIQ
                     GVLQVKESKYIMLHAPRSALKAITALLPGSESPTIIPLEGRDDKVAVHAVCRENVFWE
                     TLEQLKKAGASSMLVLPVEKMLS"
     gene            2952..4262
                     /gene="hisD"
                     /locus_tag="VFL84_15625"
     CDS             2952..4262
                     /gene="hisD"
                     /locus_tag="VFL84_15625"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011150020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET7610112.1"
                     /translation="MRVVNWESSSAALQRKLLARPALALDASVAASVAAIVERVRREG
                     DAALLDLTAKLDGVRLQSLEVGAAEIEESGTRLRAEQRGAIEAAARNIETFHRPQLPA
                     PIAVDTAKGVRCERVSRPIDSVGLYVPAGSAPLPSTALMLGVPARLAGCGTRLLCSPP
                     QADGRVHAAVLYAARVTGVQRVFKLGGAQAIAALAYGTETVPKVDKIFGPGNTWVTEA
                     KAQVDRDPAGAARDYPAGPSEVLVIADASASAAFVASDLLSQAEHGADSQVLLLTTSA
                     ELAAAVVAEIEKQKALLTRRSIVDGALASSSAIVVADLATAFAIANRYAPEHLILQIE
                     KPREWLAQVRNAGSVFLGPWTPESVGDYCSGTNHVLPTYGFARRYSSLGVGDFLRSMT
                     VQELTVEGLENIGPIATTLAELEGLDAHASAVSLRLTAAVGARR"
     gene            4259..>4614
                     /locus_tag="VFL84_15630"
     CDS             4259..>4614
                     /locus_tag="VFL84_15630"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7610113.1"
                     /translation="MKSVLDLARPELRGIKPYVPGAYEPGCIRLNANESPWRAPGDGS
                     ERGLNVYPPPRPTVLQEKLAAYYGIEQGQILVTRGSSEAIDVLIRGFCAAGRDKILVC
                     PPTFDMYRLYAGIQNAG"
BASE COUNT          742 a         1615 c         1551 g          706 t
ORIGIN      
        1 ccgatcaaag aaattcgctc gcgcacggtc gtgctgccga acgtcgacgt cgacaccgac
       61 cagatcattc cggcgcggtt tctgacgacg acgacgaagc aaggtctcgg caagaatttg
      121 ttcaacgact ggcgctatgg caaggacggc cgcgagcttc ccgacttcgt gctgaacagg
      181 cccgagaacc gcggcgcgac cgtgctcgtg gccggcaaca atttcggctg cggctcgtcg
      241 cgcgagcacg cgccgtgggc gctgctcgac ttcggcattc gcgccgtcgt cagcaccgag
      301 atcgccgata tctttcgcag caactcgctc aagaacggcc tgctgcccgt catcgtcgac
      361 gcttcgactc atcgctggct gctcgacaat cccggcgcgg acgtgcgcat cgatctcgag
      421 acgacgacgc tcgaggcgaa cggcaagcgc gtgcccttcc cgatcgaccg cttctcgcgc
      481 tactgcctca tgaacggcat cgaccagctc ggcttcctgc tcgagcagcg cgatgcgatc
      541 gcgcgctacg agcgggcgca cgcgtgagca gcaagatcgt cgtccttgcc ggcgacggcg
      601 tcggccccga ggtcaccgcc gcggccgtgc gcgtgctcga gcaagtgtcg gtccggcatt
      661 cgctgaagct cgagttcgag gacgcgctga tcggcggcgc cgcctacgac gcggccggct
      721 tgccgctgcc cgatgcgacg gtcaaggcgt gcaatgcctc aacggccgtc ctgctcggcg
      781 ccgtcggcgg cccgaaatgg gccgacatcc ccgttcaaca tcggcccgag caaggcctgc
      841 tcgcgatccg caaggcgctc ggcctgttcg cgaacctgcg ccccgtcacg acgacacccg
      901 agctcgccgc ggcgtcgccg ctgaagcccg agaaactcgc gggcgtcgac atcctcgtga
      961 tccgcgagct gacgggcggc atctacttcg gcaagcgctc gcggaccgcc gattccgcgt
     1021 tcgacacgtg cgagtacacc gccgccgaga tcgagcgcgt ctgccgcgtc gcgggcaagc
     1081 tcgcgatggc gcggcggcgc aaggtcacct ccgtcgacaa ggccaacgtg ctcgacacct
     1141 cgcgcctctg gcgcgacgtc gcgacgcgcg tgttcaagaa tgagtttccg gagcttacgg
     1201 tcgagcacct gctcgtcgac gcggccgcga tgcacctgtt gagccgcccc gccgacttcg
     1261 acgtcatggt taccgagaac atgttcggcg acatcctgac cgacgaagcc tcgatgctgg
     1321 ccggctcgat gggcctgctg ccgtccgcgt cgctgggcga cggcggcccg ggcctgtacg
     1381 agccgatcca cggctcggcc cccgacatcg cgggccaagg cgtcgcgaac ccctgcggca
     1441 cgattctgag cgcggccatg ctgctgcggc acagcgtcgg cgccgaggcg gctgcgaaag
     1501 ccgtcgaggc ggccgtggcc ggggccctcc ggtcgggcgc ccgcacgcgc gacatcgcgg
     1561 ggcccggtgc gcggaattgg ctcggaacgg cggccttcac cgacgccgtc ctcaagcatc
     1621 tagcggctcc ttgacctcgg ccttgacccg cccacggacg ttgagctacc gtactagcgc
     1681 gctattacat tagcgcgtta tttcattgct acagggacgt ccatgaagac caatcgccga
     1741 tcggcccgag acacgcagcg cgcggagcaa gggctttacc gcgcgatctt gagcctgcgc
     1801 aacgaggcgg agtgcgagca attcttcgcc gacctctgca cgccggccga gctcgaggcc
     1861 ctcgtcgacc gttgggccgt cgtcaaatat ctgcaagacg gcctacccta ccgcaagatt
     1921 catgagctca cgggcgtctc ggtgacgacc atcgggcgcg tcgcgcgctt cttgatcgcc
     1981 ggcaacggcg gttacaaaac cgcgttgaag cgcctgtcgc cgctctagga cggagaacgc
     2041 gccatgcgca tgacgacgga ctcgcgaatc aagatcgcag tgcagaaatc cggccgcctg
     2101 accgagcact cgctcgagct gctcgtgcgc tgcggcttga gctattcgcg cggcaaggac
     2161 cagctgatct gctacggcga gaacatgccc gtcgacgtgc tgctcgtgcg cgacgacgac
     2221 atcccgcgcc tcgtgcaaga ggacatctgc gacttaggga ttgtcgggct caacatcatc
     2281 gaggagcggc ggctggagct caaagaggcc ggcgagacgg agctgttcac ggagatcggc
     2341 cggctcgatt tcggtcaatg ccgtctgtcg ttcggcctgc cggccggagt gcagtacggc
     2401 ggccccaagc agctgcaggg aaagcgcatc gcgacgacgt atccgcgcat tctgcgagac
     2461 tacttgaaga agcacggcgt cgaggcgcag atcgtcgcgt tctcgggcgc ggtcgagatc
     2521 gcgccgagcc tcggcaaagc ggacttaatc tgcgatctcg tctcgagcgg cgcgacgttg
     2581 cgcgcgcatc agctctacga ggccgcgacg atcctcgaga gccgcgcggc gctgatccag
     2641 acgccgaagc cgctcacggg cgacaaggcc gaatggctgc agcgcttgct gcttcgcatc
     2701 cagggcgtgc tccaggtcaa ggaaagcaag tacatcatgc tgcacgcgcc gcgctcggcg
     2761 ctgaaggcga ttacggctct gctgccgggc agcgagtcgc cgacgatcat tccgctcgaa
     2821 ggccgcgacg acaaggtcgc cgtgcacgcc gtgtgccgcg agaacgtctt ctgggagacg
     2881 ctcgagcagc tcaagaaggc cggcgcgagc tcgatgctcg tgctgcccgt cgagaagatg
     2941 ctgtcgtagc catgcgcgtc gtgaactggg aaagctcgag cgccgccctg cagcgcaagc
     3001 tgctcgcccg ccccgcgctc gctttggacg cgagcgtcgc ggcgagcgtg gccgcgatcg
     3061 tcgagcgcgt gcgccgcgag ggcgacgcgg ccctgctcga cctgacggcg aagctcgacg
     3121 gcgtccggct gcaatcgctc gaggtcggcg ccgccgagat cgaggagtcc ggcacgcgcc
     3181 tccgcgccga gcagcgcggc gcaatcgaag ccgcggcgcg caacatcgag acgtttcatc
     3241 gcccgcagct gccggcgccg atcgccgtcg acacggccaa gggcgtgcgc tgcgagcgcg
     3301 tgtcgcggcc gatcgacagc gtcgggctct acgtgccggc cggctccgcc ccgctgccct
     3361 cgacggcgct catgctcggc gttccggccc ggctcgccgg ctgtggcacg cgtttgctct
     3421 gcagcccgcc gcaagcggac ggccgcgtgc acgcggccgt gctctacgcc gcgcgcgtga
     3481 cgggcgtgca gcgcgtgttc aagctcggcg gcgcgcaagc gatcgccgcc ctcgcctacg
     3541 gcacggagac ggtgccgaag gtcgacaaga tattcggccc cggtaacacg tgggtcaccg
     3601 aggccaaggc acaagtcgac cgcgatccgg ccggcgccgc gcgcgactac cctgccggcc
     3661 cctccgaagt gctcgtgatc gccgacgcga gcgcgagcgc cgcgttcgtc gcgtccgact
     3721 tgctgtcgca ggccgagcac ggcgccgact ctcaagtgct gctgctcacg acgagcgccg
     3781 agctcgccgc ggcggtggtc gcggagatcg agaagcagaa agcgctgctg acgcgccgct
     3841 cgatcgtcga cggcgccctc gcgagctcga gcgcgatcgt ggtcgcggat ctcgctacgg
     3901 cgttcgcgat cgcgaatcgt tacgcacccg agcacctgat cttgcaaatc gaaaagccgc
     3961 gcgagtggtt ggcgcaggtc cggaatgccg gctccgtgtt cttgggaccg tggacgccgg
     4021 agtcggtcgg cgactactgc agcggcacca atcacgtgct gccgacttac ggcttcgcac
     4081 gccgctactc gagcctcggc gtcggcgact tcctgcgctc gatgaccgtg caagagctca
     4141 cggtcgaggg cttggagaac atcggcccca tcgcgacgac gctggccgag ctcgagggcc
     4201 tcgacgcgca cgcgtccgcg gtcagcctgc ggcttacggc cgcggtggga gcgcggcgat
     4261 gaagagcgtg ctcgacctcg cgcggcccga gctgcgcggc atcaagccgt acgtgcccgg
     4321 cgcgtacgag cccggctgca ttcgcctgaa cgcgaacgag tcgccgtggc gcgcgcccgg
     4381 cgacggctcc gagcgcggcc tcaacgtcta tccgccgccg cggcccacgg tgctgcagga
     4441 gaagctcgcc gcctattacg gcatcgagca gggccagatc ctcgtgacgc gcggcagcag
     4501 cgaggcgatc gacgtgctga tccgcgggtt ctgcgctgcg ggccgcgaca agattctcgt
     4561 ctgcccgccg acgttcgaca tgtatcgcct ctacgccggc attcagaacg ccgg
//
LOCUS       DASSNN010000834         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1754569, whole genome shotgun sequence.
ACCESSION   DASSNN010000834 DASSNN010000000
VERSION     DASSNN010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754569"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1632
                     /locus_tag="VFL84_15635"
     CDS             <1..1632
                     /locus_tag="VFL84_15635"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7610114.1"
                     /translation="FADNSDDVYVALQLSESMATASYRRVRDFLFAITGVSLLLAISG
                     SFGLAKTVTRPVHDLAEAARRMREGVYNEPINIRTGDEFGELAGSFNAMQQAIADRER
                     RIVHQAHHDSLSGLPNREAVVGLLRDAIERHDKLAVVSLGLDRVNGIVSSLGHRAGDE
                     VVKLAAAALRSRLGDTEVLGHLSAHEFVVGLPGRDAREAVEWIEFQADALRAGVRVSN
                     ANIALQATGGVACYPEHSEDAAELCRRASSARSAALALHQTAAVYRLGQDDRSLQQIK
                     IVGDFPRALRDNELRLYMQPKLDLTTGEIYGAEALVRWQHPELGLLYPDAFISAVEQA
                     GSIAHLTRWVLREAVGRCAAWRNQGVILGVAVNISVDDLTDEYLPYFLLELTQKHHLA
                     PHMLTLEVTESAIMHNVQKSLAVVNCIHELGFRIGVDDFGTGHSALAQLKRLPVDELK
                     IDKSFVTRAEDAKDDAILRATIDLAHQLGLTIVAEGVEDEATITRLAAMGCEHAQGYG
                     IAKPMPQEQLLSWLAQRRSGGRSSVIALNAAGNTA"
     gene            complement(1671..2489)
                     /gene="tsaA"
                     /locus_tag="VFL84_15640"
     CDS             complement(1671..2489)
                     /gene="tsaA"
                     /locus_tag="VFL84_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HET7610115.1"
                     /translation="MTEPQRRAASARQLVLEPIGYARTELATKVEAARQPRAGSGAVG
                     RIELLPARNFEHALSNLAEWQYIWVLFWFDRNEGWRPKVLPPRSRSGRKGVFATRSPH
                     RPNPLGLSAVRLERVEGLTLHVSDVDMLDGTPVLDIKPYVAYTDAIAEAGAGWLTSEQ
                     APRDPLPEFEVEWDGVAAEQAVWVEAKTGLPLRTRATATLALGPEPHPYRRIRRERDG
                     FTLCVKDWRLRFRVDGRRVHVAAVESGVRKSLLNGHGDADAELLLHREFFSRWR"
BASE COUNT          430 a          929 c          926 g          419 t
ORIGIN      
        1 ttcgccgaca acagcgacga cgtgtacgtc gcgctgcagc tgtcggagtc gatggccacg
       61 gcgtcgtacc gccgcgtccg tgatttcctg ttcgcgatca ccggcgtctc gctgctgctc
      121 gcgatcagcg gctcgttcgg gctcgcaaag acggtgacgc ggcccgtgca cgatctcgcc
      181 gaggccgcgc gccgcatgcg cgaaggcgtc tacaacgagc cgatcaacat ccgcacgggc
      241 gacgagttcg gcgagctcgc cggcagcttc aacgcgatgc agcaagcgat cgccgaccgc
      301 gagcggcgca tcgttcatca ggcgcatcac gattcgctgt ccggcttgcc gaaccgcgag
      361 gccgtcgtcg ggcttctgcg cgacgcgatc gagcgccatg acaagctcgc ggtcgtgagc
      421 ttggggctcg accgcgtcaa cggcatcgtg tcgtcgctcg gccaccgcgc cggcgacgag
      481 gtcgtcaagc tcgcggccgc cgcgctgcgc agccgcttgg gcgacaccga ggtgctgggc
      541 cacttgagcg cgcacgagtt cgtcgtaggg ctgcccggcc gcgacgcgcg cgaggccgtc
      601 gagtggatcg agttccaggc cgacgcgctg cgcgcgggcg tgcgcgtgtc gaacgccaac
      661 attgcgttgc aagccacggg cggagtcgcg tgctatcccg agcacagcga ggacgccgcc
      721 gagctttgcc gccgcgcgtc gagcgcgcgc agcgccgcgc tcgcgctcca ccagacggcg
      781 gccgtctacc gcctgggtca ggacgaccgc tcgctgcagc agatcaagat cgtcggcgac
      841 tttccgcgcg cgttgcgcga caacgagctg cgtctctaca tgcagccgaa gctcgacctc
      901 acgacgggcg agatctacgg cgccgaggcg ctcgtgcgct ggcagcatcc cgagctcggg
      961 ctcttgtacc ccgacgcgtt catctccgcc gtcgagcaag ccggcagcat cgcgcacctg
     1021 acgcgctggg tgctgcgcga ggccgtcggg cgctgcgcgg cgtggcgcaa ccagggcgtc
     1081 atcttgggcg tcgccgtcaa catctccgtc gacgacttga cggacgagta cctgccgtat
     1141 ttcttgctcg agctgacgca gaagcatcac cttgcgccgc acatgttgac gctcgaggtg
     1201 acggagagcg cgatcatgca caacgtgcag aagtcgctcg ccgtcgtgaa ctgcatccac
     1261 gagctcggct tccggatcgg cgtagacgat ttcggaacgg gccactcggc gctcgcgcag
     1321 ttgaagcgcc tgcccgtcga cgagctcaag atcgacaagt cgttcgtcac gcgcgccgag
     1381 gacgcgaagg acgacgcgat cctgcgcgcg acgatcgact tggcgcacca gctcgggctc
     1441 acgatcgtcg ccgaaggcgt cgaggacgag gcgacgatca cgcggctcgc ggcgatgggg
     1501 tgcgagcacg cgcagggcta cggcatcgcc aagccgatgc cgcaggaaca gctcctctcg
     1561 tggctcgcgc agcggcgcag cggcggccgc tcgagcgtga tcgcgctgaa tgccgccggc
     1621 aatacggcgt agtcgcgcga cgcgttggcc tctaggaaag catggcggcg tcagcgccag
     1681 cgcgagaaga actcgcgatg caatagaagc tctgcgtcgg catctccgtg cccgttcaac
     1741 agcgacttgc ggacgcccga ttcgacggcc gcgacgtgaa cacggcgccc gtcgacgcgg
     1801 aagcggagcc gccaatcctt gacgcacagc gtaaatccgt ctcgctcgcg gcgaatccgc
     1861 cgatacggat gcggctcggg cccgagcgcg agcgtcgcag tcgcgcgcgt gcgcagcggc
     1921 aagccggttt tcgcttcgac ccagaccgcc tgctcggcgg ccacgccgtc ccactccact
     1981 tcaaactcgg gtagcggatc gcgcggcgct tgctcgctcg tgagccagcc ggcgccggct
     2041 tccgcgatcg cgtcggtgta ggcgacgtac ggcttgatgt cgagcacggg cgtgccgtcg
     2101 agcatgtcga cgtcgctgac gtgcagcgtg aggccctcga cgcgctcgag ccgaaccgcg
     2161 gacaggccga gcggattcgg ccggtgcggc gagcgcgtcg cgaacacgcc cttgcgtccc
     2221 gagcggctgc gcggcggcag cactttcggc cgccaaccct cgttgcgatc gaaccagaac
     2281 agcacccaga tgtactgcca ttcggcgagg ttcgagagag cgtgctcgaa gttgcgcgcg
     2341 ggtagaagct cgatgcgccc gacggcgccg ctgccggcgc gcggctggcg cgcggcctcg
     2401 acctttgtcg caagctccgt gcgcgcgtag ccgatcggct cgagcacgag ctgtcgtgcg
     2461 gacgctgcgc gtcgttgcgg ctcggtcacg cgcacctcgt ggagttgcac acgcgtgaac
     2521 tttacatcac gccgtcaaac tgagggacgc atcactcggc cggcagcgcg gatcccctat
     2581 gctcgtaagc tcattccagc cggctcgtgt gcgtgatgac ggatcatgat tcgcgtcttc
     2641 agggcatcgc gccaatcgaa gcggatcgcg acggcgtaaa cgtcggcgag agccttttgc
     2701 cggc
//
LOCUS       DASSNN010000835         2699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1376027, whole genome shotgun sequence.
ACCESSION   DASSNN010000835 DASSNN010000000
VERSION     DASSNN010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376027"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFL84_15645"
     CDS             <1..622
                     /locus_tag="VFL84_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HET7610116.1"
                     /translation="NAEIKARVEQIRRQVEEATSDYDKEKLQERVAKLSGGVAVIKVG
                     AATEVEMKEKKARVEDALHATRAAVEEGVVPGGGVALIRALKGIANLEGANEDQNVGI
                     NILKRAIEEPLRQIVANAGTDPAVVLNAVVNGKGNYGFDAATGEYGDMVKLGIIDPTK
                     VTRLALQNAASVAGLLLTTEAMIAEAPKQEKEAVGGPHGHGMDGMM"
     gene            complement(683..1639)
                     /locus_tag="VFL84_15650"
     CDS             complement(683..1639)
                     /locus_tag="VFL84_15650"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7610117.1"
                     /translation="MTPRLQTQLTGSQEPRLEQDAAVMEDGARLPLRSWTPVAAAPHA
                     MIVAVHGLNDYSGGFERTGAFLAARGFAVYAFDQRGFGRAPQTGIWAGGDRMADDARE
                     VARLLRIRHPDVPVYALGESMGGAVLLDALKRHPRGWIDAVALSAPAVWGRSEMRRYQ
                     RMPLNLLAHSVRGLKLSGRITGRRPSDDPATLEWLREDPLVIRRARVDVLWGLADLMD
                     AVTLAPLKPSVPVLVLYGAHDQIVPPAPMCAWLTALPDSGEWQPALYPSGWHLLTRDR
                     DAARALGDLAAWFENPGSGLPSGADLNDPAARVCALAGETGT"
     gene            1840..>2699
                     /locus_tag="VFL84_15655"
     CDS             1840..>2699
                     /locus_tag="VFL84_15655"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamine synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HET7610118.1"
                     /translation="MILPRPVDLPAALEARFAHVSARLADLEVDLPPPAAKTAARVVV
                     VSDFVLGALLRHSQALSARLADDGPLDPQSIAARLELDAADEAAAMQRLRRTREIEMA
                     RIAWRDLAGLADLETTLADVSLLAECLIDAALRYAAASLEPRLGRPRNANGDELPLLV
                     LGMGKLGGAELNFSSDVDLVFLHPDGAVEGSLKGERETYYLRLGQLLIKLLDQRTADG
                     FAYRVDMRLRPFGTSGPLAIGLAAFEAYLMQHGRDWERYAYVKARLLTGVQFASDVFD
                     YVLTPFVYRR"
BASE COUNT          448 a          912 c          938 g          401 t
ORIGIN      
        1 gaacgccgag atcaaggcac gcgtcgaaca gatccgtcgc caggtcgaag aagccacttc
       61 ggattacgac aaggaaaagc tgcaggagcg cgttgccaag ctctccggcg gtgtcgccgt
      121 gatcaaggtc ggcgctgcga ccgaagtcga gatgaaggag aagaaggccc gcgtcgagga
      181 cgcgctgcac gcgacgcgcg cggccgtcga agagggcgtc gtgccgggcg gcggcgtggc
      241 gctgatccgc gcgctgaagg gcatcgcgaa cctcgagggc gcgaacgagg accagaacgt
      301 cggcatcaac atcttgaagc gggccatcga agagccgctg cgccagatcg tcgccaacgc
      361 gggcacggac ccggccgtgg tgctcaacgc cgtcgtgaac ggcaagggca actatggctt
      421 cgatgccgcc accggcgagt acggcgacat ggtcaagctc ggcatcatcg acccgaccaa
      481 ggtcacgcgt cttgcgttgc agaacgcggc gtccgtcgcg ggtctcttgc tgacgaccga
      541 ggcgatgatc gccgaggctc cgaagcagga gaaggaagcc gtcggcgggc cgcatggcca
      601 cggcatggac ggaatgatgt aagtcctttt cgagcacgca cgctcgaacc gaaagccccg
      661 cctcgcgcgg ggctttcctt ttctaggtcc ccgtctcgcc ggcgagcgcg cacacgcgcg
      721 ctgcggggtc attgagatcc gcgccggacg gcaagccgct gccgggattc tcgaaccacg
      781 cggcgagatc gccgagcgcg cgtgccgcat cgcggtcgcg cgtcagcaag tgccagccgc
      841 tcggatagag cgccggttgc cattcgcccg aatcgggcaa ggccgtaagc cacgcgcaca
      901 tcggcgcggg cggaacgatc tggtcgtgcg caccgtagag aacgagcacg gggaccgacg
      961 gcttcagcgg cgcgagtgtc acggcgtcca tgagatcggc gaggccccag agcacgtcga
     1021 cgcgcgctcg acggatcacg agcgggtctt cgcgaagcca ttcgagcgtc gcgggatcgt
     1081 cggacggcct ccgcccggtg atccgtcccg agagcttgag acctctcacg ctgtgcgcga
     1141 gcaggttcag cggcatgcgc tggtagcggc gcatctcgct gcggccccag acggccggcg
     1201 cgctcagggc aaccgcgtcg atccatcctc gcggatggcg cttcagcgca tcgagaagca
     1261 cggcgccgcc catgctttca cccaacgcgt agacgggaac gtcgggatgg cgaatgcgca
     1321 gcaagcgcgc gacctcgcgc gcatcgtcgg ccatccggtc gccgccggcc caaatgcccg
     1381 tctgcggcgc tcggccgaag ccgcgctggt cgaaggcgta gacggcgaag ccgcgcgcgg
     1441 cgagaaaggc gccggttcgt tcgaagccgc cggagtagtc gttgagcccg tgcacggcga
     1501 cgatcatggc gtgcggagcc gcggcgacgg gcgtccacga gcgcagcggc agacgcgcgc
     1561 cgtcctccat taccgcggcg tcttgctcta accgcggctc ttgcgagccg gtcagctgcg
     1621 tttgcaggcg cggcgtcacg catgccgcgt gcacggagca cgagagcgcc gcgagcgcga
     1681 ggcgcgcgag agctctgcct cgcgcgccgg acctccgtag cgaagacccc atgccgcgat
     1741 agtgcagacg tttcgtcgcg cgcagcaata ggccggcggc gctcgaagcg cgctcgcaag
     1801 cggtatcatg ctcgagtttt cggcgccttt gatcccacgg tgatactgcc acgccccgtt
     1861 gatctcccag cggcgctcga agcgcgcttc gcgcacgtct cggcacggct cgccgacttg
     1921 gaggtcgact tgccgccgcc cgcagccaag acggccgctc gcgtcgtcgt cgtcagcgac
     1981 ttcgttctcg gcgctctgct gcgtcactcg caggcactca gcgcgcgcct cgccgacgac
     2041 gggccgctcg acccgcagtc gatcgcggcg cggctcgagc tcgacgcggc agacgaagcg
     2101 gccgcgatgc aaaggctgcg gcgcacgcgc gagatcgaga tggcacggat tgcgtggcgc
     2161 gacctggccg gccttgccga tctcgaaacg acgctcgcgg acgtttcgtt gctcgcggag
     2221 tgcctcatcg acgccgcgct tcgctacgcc gccgcgagcc tcgagccgcg gctcgggcgc
     2281 ccgcgtaacg cgaacggcga cgagctgcca ttgctcgtgc tcggcatggg caagctcggc
     2341 ggcgccgagc tcaacttctc gtcggacgtc gacttggtgt tcttgcatcc ggacggagcg
     2401 gtcgaaggta gtctcaaagg cgagcgcgag acctactatc tgcgcctcgg gcagttactg
     2461 atcaaattgc tcgatcagcg caccgcggac ggcttcgcgt atcgcgtcga catgcggctg
     2521 cggccgttcg gcacgagtgg gccgctcgcg atcggcctag cggcattcga ggcatacctg
     2581 atgcagcacg gccgcgattg ggagcgctac gcctacgtca aggcgcggct gctcacgggc
     2641 gtgcaattcg cttcggacgt cttcgactac gtgctgacgc cgttcgtcta tcgccgcta
//
LOCUS       DASSNN010000836         4226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_745699, whole genome shotgun sequence.
ACCESSION   DASSNN010000836 DASSNN010000000
VERSION     DASSNN010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745699"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /gene="speA"
                     /locus_tag="VFL84_15660"
     CDS             complement(<1..972)
                     /gene="speA"
                     /locus_tag="VFL84_15660"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HET7610119.1"
                     /translation="MSTQPQTKPSPVEWQPSDAAELYRIPSWGDPFFFVNDKGHMAVR
                     SLDEHGTTMDIVDIVNELRRRGVQFPVLLRFQDVLRAQVRRINEAFRSAIAESNYGNV
                     YRGIYPIKVNQLHEVVDELLDAGRPYGMGLECGSKAELIACLPHIDGETLLVCNGVKD
                     RTMLSLMIAGQKLGQNIVPVMEKYSEFVDVKGLAFSTGYMSQLGVRVRLATRGAGRWA
                     ESSGTNSKFGLNVSELMRMVNELEASGQQSKLVLLHCHIGSQIADIQVLKEATKEITQ
                     IYAQLVKRGLALKYLDLGGGLGVNYDKGHLEEDAGINYALEEYANAVV"
     gene            complement(977..1948)
                     /locus_tag="VFL84_15665"
     CDS             complement(977..1948)
                     /locus_tag="VFL84_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HET7610120.1"
                     /translation="MGAISDFIRHHYRHFNGAALVDAADAYVKHLDAGGQMLVTLAGA
                     MSTAELGLSLAEMIRRGKVHAISCTGANLEEDIFNLVAHDHYVRIPNYRYLTPDQEEE
                     LLDQHLNRVTDTCIPEHEAIRRIEKAVLDEWQRADKAHQQFFPHEFMFKILRSGVLEE
                     HFQIDPKDSWMVAAAERDLPMFVPGWEDSTLGNIYAGHCIAGDIDNVHTVRSGIEYMM
                     SLADWYQATCRKGSIGFFQIGGGIAGDFPICVVPMLQQDLQLADTPRWGYFCQISDST
                     TSYGSYSGAVPNEKITWGKLSASTPKFIIESDATIVAPLIFAHVLGE"
     gene            complement(2035..3117)
                     /locus_tag="VFL84_15670"
     CDS             complement(2035..3117)
                     /locus_tag="VFL84_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HET7610121.1"
                     /translation="MTGDELSADHADTPKARGYRQPAEWAPHEATWLSWPHNRDSWPG
                     VFERAEPAMVELVRALAKCEPVYINVLDGDHERHVRKLLARVASSAALRFYRFPTNDA
                     WARDHGAIFVTRPSAKEPRLAVDFDYNAWGGKYPPFDLDREIGRQMAEALGVPRYAIP
                     NVVLEGGSIDVNGEGALLTTEQCLLNPNRNPTLTRADIERLLCDCLGVDEILWLGEGI
                     EGDDTDGHIDDLTRFVGPRTVVTVVEPNRADPNHAPLAANRRLLDTLSVGGGRLTVVE
                     LPMPEPQYLEEQRLPASYANFYVANGAVLVPTFGCPADEVACATLRDCFPGRRVVGVD
                     CRVLIAGLGALHCLTQQVPAAPAVAS"
     gene            3239..3871
                     /locus_tag="VFL84_15675"
     CDS             3239..3871
                     /locus_tag="VFL84_15675"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET7610122.1"
                     /translation="MTTPKPRRNVSAWVAALGATFAFAAAPLTAQTVSTGTLLVASPQ
                     LTDVNFARSVVLVLQNDENGAVGVVLNRPTNLVPATVFPELAEGIGAYDGRLFRGGPI
                     APTRVLYLVRGLAAATVTGPEIVDKVFLSIDDASLPDMVRLADGTDDLRLYAGHAAWV
                     PGQLQAEINAGGWQVLLATPELVFDEDPATLWTKLEGQGSSGSNVVAAAQ"
     gene            complement(3930..>4226)
                     /locus_tag="VFL84_15680"
     CDS             complement(3930..>4226)
                     /locus_tag="VFL84_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610123.1"
                     /translation="DGVQVIRGTPTAPITSPTLFLRIWQVNEDKHVRTRSTLFMVRPG
                     PGDYVIKELIPDMELDVKAALEKAVAIAKRGGVSVVYLNADLAKLPQVRIARAS"
BASE COUNT          684 a         1402 c         1422 g          718 t
ORIGIN      
        1 cacgaccgcg ttcgcgtatt cctccagcgc atagttgatg ccggcgtctt cctcgaggtg
       61 gcctttgtcg tagttcacgc cgaggccgcc gccgaggtcg aggtacttga gcgccagccc
      121 acgcttcacg agctgtgcgt agatctgcgt gatctctttc gtcgcttcct tcagcacttg
      181 gatgtccgcg atctggctgc cgatgtggca gtgcagcagc acgagcttcg attgctggcc
      241 gctcgcctcg agctcgttga ccatgcgcat gagctccgac acgttcaggc cgaacttcga
      301 gttcgtgccc gacgattccg cccaccgtcc cgcgccgcgc gtcgcgagcc gcacgcgcac
      361 gccgagctgc gacatgtagc ccgtcgagaa cgcgagacct ttgacgtcga cgaactccga
      421 gtacttctcc atgacgggca cgatgttctg cccgagcttc tggcccgcga tcatcagcga
      481 cagcatcgtg cggtccttga cgccgttgca gacgagcagc gtttcgccgt cgatgtgcgg
      541 caagcacgcg atcagctccg ctttggagcc gcactcgagg cccatgccgt acggccggcc
      601 cgcgtcgagc agctcgtcga cgacctcgtg cagctggttg accttgatgg ggtagatgcc
      661 gcgatagacg ttgccgtagt tcgattccgc gatcgcgctg cggaacgcct cgttgatgcg
      721 ccgcacctgc gcgcgcagca cgtcctggaa gcgcagcagc acgggaaact gcacgccgcg
      781 gcggcgcagc tcgttgacga tgtcgacgat gtccatcgtc gtgccgtgct cgtcgagcga
      841 gcgcacggcc atgtggccct tgtcgttgac gaaaaagaac gggtctcccc agctcggaat
      901 ccgatagagc tccgcagcgt ccgacggctg ccattcgacg ggcgacggtt tggtctgggg
      961 ctgtgtggac atctggttac tctccgagca cgtgagcgaa aatcaacggc gcgacgatcg
     1021 tcgcgtcgga ctcgatgatg aacttcgggg tcgaagccga gagcttgccc cacgtgatct
     1081 tctcgttcgg caccgcgccc gagtaggagc cgtagctcgt cgtcgagtcg ctgatctggc
     1141 agaaatatcc ccagcgcggc gtgtcggcga gctgtaggtc ttgctgcagc atcggcacga
     1201 cgcagatcgg gaaatcgccg gcgatgccgc cgccgatctg gaagaagccg atcgagcctt
     1261 tgcggcaggt ggcctgatac cagtcggcga ggctcatcat gtactcgatg ccgctgcgaa
     1321 cggtgtgcac gttgtcgatg tcgcccgcga tgcagtggcc ggcatagatg ttgccgagcg
     1381 tcgagtcctc ccagccgggc acgaacatcg gcaggtcgcg ctccgcggcc gcgaccatcc
     1441 agctgtcctt gggatcgatc tggaagtgct cctcgaggac gcccgagcgc aggatcttga
     1501 acatgaactc gtgcgggaag aattgctggt gcgccttgtc ggcacgctgc cactcgtcga
     1561 gcacggcttt ctcgatgcgg cgaatcgcct cgtgctcggg gatgcaggta tcggtgacgc
     1621 ggttcaagtg ctggtcgagc agctcttctt cctggtccgg cgtcaggtac cggtaattcg
     1681 gaatccgcac gtaatgatcg tgcgcgacga ggttgaagat gtcctcctcg agattcgcgc
     1741 ccgtgcacga gatcgcgtgg accttgccgc gccggatcat ctcggcgagc gacaggccga
     1801 gctcggccgt gctcatggcg ccggcgagcg tgacgagcat ctgcccgccc gcgtcgaggt
     1861 gcttgacgta ggcgtccgcg gcatcgacga gcgcggcgcc gttgaaatgc cgatagtggt
     1921 ggcgaatgaa gtccgagatc gcgcccatcg agatacctca ggcttcttca gcgcccgtcg
     1981 aaagcgtgaa gattaggaaa tcgggtctaa acccacaacc ccggccgcaa atcgtcacga
     2041 ggccaccgcc ggcgccgcgg gcacctgctg cgtgaggcag tgcagcgcgc cgagtcccgc
     2101 gatcaacacg cggcaatcga cgccgacgac gcggcgcccc ggaaaacaat cgcgcagcgt
     2161 cgcgcaggcg acctcgtccg ccgggcagcc gaacgtcggc acgagcacgg cgccgttcgc
     2221 gacatagaag ttcgcgtagc tcgccggcaa gcgctgctcc tcgaggtatt gaggctcggg
     2281 catcggcaac tcgacgacgg taaggcgccc gccgccgacg gacaaggtgt cgagcagccg
     2341 gcggttcgcc gcgagcggcg catgattcgg atcggcgcgg ttcggctcga cgacggtcac
     2401 gaccgtgcgg ggcccgacga agcgcgtgag gtcgtcgatg tggccgtcgg tgtcgtcgcc
     2461 ctcgatgccc tccccgagcc agaggatctc gtcgacgccg agacagtcgc agagcagccg
     2521 ctcgatgtcg gcgcgcgtca acgtggggtt gcggttcggg ttcagcaggc attgctccgt
     2581 cgtcagcagc gcgccctcgc cgttgacgtc gatcgagccg ccctcgagca cgacgttcgg
     2641 gatcgcgtag cgcggcacgc cgagcgcctc ggccatctgc cggccgatct cgcggtccaa
     2701 gtcgaacggc gggtacttgc cgccccaggc gttgtagtcg aagtcgacgg cgagccgcgg
     2761 ctccttcgcc gacggccgag tcacgaagat ggcgccgtgg tcgcgtgccc aggcgtcgtt
     2821 ggtcggaaaa cggtagaacc gcaaggccgc agacgaagcg acgcgcgcga gcagcttgcg
     2881 tacgtggcgc tcgtggtccc catccagcac gttgatatag acgggctcgc acttggcgag
     2941 cgcgcgcacg agctcgacca tggccggctc ggcgcgctcg aatacgccgg gccagctgtc
     3001 gcggttgtgc ggccacgaca gccacgtcgc ctcgtgcggc gcccattcgg cgggctggcg
     3061 gtaaccgcga gccttaggcg tatcggcgtg gtcggcggac aattcgtctc ccgtcatggc
     3121 gtcgagccgc ggcatgacga tcatgccggg gccacccaaa gggcgcgcat catacgccgt
     3181 cgatccgagg atcgatacac ccgtgggccg tggacacggt tatcatgccg cgatgacgat
     3241 gacgactccg aaaccacgtc gaaacgtaag cgcctgggtc gcggcgttgg gcgcgacgtt
     3301 cgcctttgcg gcggcgccgc tcacggctca gacggtcagc accggcacct tgctcgtcgc
     3361 gtcgccgcag ctgaccgacg tcaacttcgc gcgctcggtg gtgctcgtgt tgcagaacga
     3421 cgagaacggc gcggtcggcg tcgtgctgaa ccggcccacg aatctcgtgc cggcgaccgt
     3481 gttcccggag ctcgccgaag gcatcggcgc ctacgacggc cgcctcttcc gcggcggacc
     3541 gatcgcgccc acacgcgtgc tctacctcgt ccgcggcctc gcggcggcca ccgtgaccgg
     3601 ccccgagatc gtcgacaagg tgttcttgag catcgacgac gcgtcgctgc ccgacatggt
     3661 gcggctcgcc gacggcaccg acgaccttcg gctctacgcc ggtcatgcgg cgtgggttcc
     3721 gggacagctg caagccgaga tcaacgccgg cggctggcaa gtgctgctcg caacgcccga
     3781 gctcgtgttc gacgaggacc cggccaccct ctggaccaag ctcgaaggcc agggcagcag
     3841 cggcagcaac gtcgtggcag ccgcccagta ggcacgcccg gctcgcgccg cggccggtca
     3901 ggcggctcgt cggcgcccgg cgccggcgat caagaggcgc gcgcgatgcg gacctgggga
     3961 agcttggcga ggtcggcgtt caagtagacg acggacacgc cgccgcgctt cgcgatcgcg
     4021 acggccttct cgagcgcggc tttgacgtcg agctccatgt cggggatcag ctctttgatg
     4081 acgtagtcgc cgggcccggg acgaaccatg aacagcgtcg agcgcgtgcg cacgtgcttg
     4141 tcttcgttga cttgccagat gcgcaagaac agcgtcgggc tcgtgatcgg cgccgtcggc
     4201 gtcccccgaa tcacttgcac gccgtc
//
LOCUS       DASSNN010000837         4330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_745723, whole genome shotgun sequence.
ACCESSION   DASSNN010000837 DASSNN010000000
VERSION     DASSNN010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745723"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2140)
                     /locus_tag="VFL84_15685"
     CDS             complement(<1..2140)
                     /locus_tag="VFL84_15685"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HET7610124.1"
                     /translation="MALMRGIVRATTIAFIGVALLAAFSAGGFAFLVRKLPSYQDDIQ
                     AWVTAKLGLTLDFARLEGGWGWRGPEIAFREVSVRAAGDETPFLTARGASVGFNVLDL
                     GWRLATGREIAIDRLTFDGTEFTLVRNEQGAYRLQGAPAISGEQATVQVPPDIDVLVR
                     DSRVLYLDDSRSVAWAFQDVTASLRRDDEVLTLEAQALPPVEFASHIDIAAQAVVGDD
                     DSGAKFTGEWRMSADLDEVDLAVAAQLFPPSAVAPRAGRGDVAVWLELQGGAVKSGTV
                     ELALADVALQSTLGTVDSRFERIAFSGTWEQSDEALRLSLHDVAVTRSGRAWPEAATV
                     DIDVGRDRDGVNRVALRSSFLRLEDLTPFFAPLPESRVLESWFALAPRGDLRAVDVAL
                     TRNADARVEYSVAAQFQGLGVDRFEDAPGITGLTGQVRADSRAGRLELASEGAALDWP
                     ALFRSALSVPELKGIVVWRAGQDAVRVVSDDLLVATPAASLRTNLELTLPMDDSSARL
                     DLRTSVSEFDIAAAPRYLPANKMPATVVAWLDSALRGGRAKSAEVTFVGPVRAFPFDG
                     GEGEFRATLQVEDVVLAFVKDWPAAEELDGTVEFVNARFAARGSGRVLGNRTADVRVG
                     IGDLRAGDFTLQAETIGGLDQVLAFLNGAPLIAGYLGEDFARLEAPSGTAATKVDLAL
                     PLRDRDAYRLTAGLDIIDGELAYRGFGPQVS"
     gene            complement(2143..3615)
                     /gene="rng"
                     /locus_tag="VFL84_15690"
     CDS             complement(2143..3615)
                     /gene="rng"
                     /locus_tag="VFL84_15690"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HET7610125.1"
                     /translation="MKREEILINATSTEVRAALVDSGVLQEVQIERASRVGLVSNIYK
                     GKVSRVLPGMNAAFVELGLERTAFLHASDIAVNPHAEPGPAREVEPDIREFVREGQEL
                     LVQVLKEPLGTKGARLTTYIAIPSRYLVLTPNSLGVGVSSRIDDEAERERLRSTLEEI
                     KAEAPPAGYIIRTAAEGATREALQADVMFLHKLWQSIEQKAEKTPAGGLVYEDLPLPL
                     RVLRDSSSPDIARVRVDSEATFAKLKEFADHFVPRLSNLIELYTEPRPIFDVYGIDDE
                     IDRALHRKVPLKSGGHVVFDQTEAMTTIDVNTGGYVGHRNLEETIFRTNLEAAAAIAR
                     QLRLRNLGGIIIIDFIDMTEEEHRRQVLQTLTDRLAGDHAKSHVMSVSPLGLVEMTRK
                     RNRESLEHVLCQACPSCEGRGFVKTAETVCHEIFREILRQHRQFDFQELLVLARPQVI
                     ERLLDEDSAGIAELEEVTGKPIRLQSEALYSPEQFDVVLM"
     gene            complement(3615..4214)
                     /locus_tag="VFL84_15695"
     CDS             complement(3615..4214)
                     /locus_tag="VFL84_15695"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET7610126.1"
                     /translation="MKKDFVYLASASPRRSELLRQIGVRFTVRAAAIPEEQLPRETPE
                     AYVTRLATAKAESVWAVTVGDLRPVLGADTAVVLDGQVLGKPETEAEAAAMLERLSGR
                     THRVLTAVALRHAGGLETASSETEVRFRATTAAERLAYCRTGEPFDKAGGYGIQGHAA
                     VFVEEIKGSYSGVVGLPLFETAGLLTRCGVDFWLAARAA"
BASE COUNT          654 a         1531 c         1475 g          670 t
ORIGIN      
        1 ccgacacctg cgggccgaag ccgcggtacg cgagctcgcc gtcgatgatg tcgagccccg
       61 ccgtgagccg gtaagcgtcg cggtcgcgca gcggcagcgc caggtcgact ttcgtcgccg
      121 ccgtgccgct cggcgcctcg aggcgcgcga agtcctcgcc gagataaccc gcgatcaacg
      181 gcgcgccgtt caagaacgcg agcacttgat cgaggccgcc gatcgtctcg gcctgcagcg
      241 tgaagtcgcc ggcgcgcagg tcgccgatgc cgacgcgcac gtcggccgtg cggttgccga
      301 gcacgcggcc gctgccgcgc gccgcgaacc gcgcgttgac gaactcgacg gtgccgtcca
      361 gctcctcggc ggcgggccag tccttgacga aggcgagcac cacgtcctcg acctggagcg
      421 tcgcgcggaa ttcgccctcg ccgccgtcga acgggaacgc gcgcaccggc ccgacgaacg
      481 taacctccgc gctcttcgcg cggccgccgc gcagcgccga atcgagccac gccacgacgg
      541 tcgcaggcat cttgttcgcc ggcaagtagc gcggcgccgc cgcgatgtcg aactccgaca
      601 cgctcgtgcg cagatcgagc cgcgcggagc tgtcgtccat cggcagcgtc agctcgagat
      661 tcgtgcgcag cgaggcggcc ggcgtcgcaa cgagcaggtc gtcgctgacg accctgaccg
      721 cgtcctggcc ggcgcgccag acgacgatgc ccttgagctc gggcacgctg agcgcgctgc
      781 ggaacagcgc gggccaatcg agcgcggcgc cttcgctcgc gagctcgagc cttccggcgc
      841 gcgaatcggc gcggacctgc cccgtgaggc cggtgatgcc gggtgcgtcc tcgaagcggt
      901 cgacgccgag cccttggaac tgcgcggcca cggagtactc gacgcgcgca tcggcattgc
      961 gcgtcaacgc gacgtcgacg gcgcgcagat cgccgcgcgg cgccagagcg aaccacgact
     1021 cgagcacgcg ggactcgggc agcggcgcga agaacggcgt gaggtcttcg aggcgcagga
     1081 agctgctgcg cagcgcaacg cggttgacgc cgtcgcggtc gcggccaacg tcgatgtcga
     1141 cggtcgcggc ttccggccac gcgcggccgc tgcgcgtgac ggcgacgtcg tgcagcgaca
     1201 gccgcagcgc ttcgtcgctc tgctcccacg tgcccgagaa cgcgatacgc tcgaaccgcg
     1261 agtcgaccgt gccgagcgtg ctctgcagcg cgacgtccgc gagcgcgagc tcgacggtgc
     1321 cgctcttgac cgcgccgcct tgcaactcga gccatacggc cacgtcgccg cgcccggccc
     1381 gcggcgccac ggccgacggc gggaacagct gcgcggcgac ggcgaggtcg acctcgtcga
     1441 gatcggccga cattcgccat tcgcccgtga acttggcgcc cgaatcgtcg tcgccgacga
     1501 cggcttgcgc ggcaatgtcg atgtggctcg cgaactcgac cggcggcagc gcttgcgcct
     1561 cgagcgtcag cacctcgtcg tcccggcgca ggctcgccgt gacgtcctgg aacgcccacg
     1621 cgacgctgcg cgaatcgtcg agatagagca cgcggctgtc gcgcacgagc acgtcgatgt
     1681 cgggcggcac ttgcacggta gcctgctcgc cgctgatcgc gggcgcgcct tgcagccgat
     1741 aggcgccctg ctcgttgcga acgagcgtga actcggtgcc gtcgaacgtg agccggtcga
     1801 tggcgatctc ccgcccggtc gcgagccgcc agcccaagtc gagcacgttg aagccgacgc
     1861 tcgcgccgcg cgccgtcaag aacggcgtct cgtcgccggc cgcgcggacg ctcacctcgc
     1921 ggaacgcgat ctcggggccg cgccatcccc acccgccttc gaggcgcgcg aaatcgagcg
     1981 tgagcccgag cttcgccgtg acccaggctt gaatgtcgtc ctggtagctc ggcagcttgc
     2041 gcacgaggaa cgcgaacccg cccgcgctga acgcagcgag caaggcgacg ccgatgaaag
     2101 cgatcgtcgt tgcccgcacg attccgcgca tcaatgccat gactacatca gcaccacgtc
     2161 gaattgctcc ggcgaataga gcgcctcgct ctgcagccgg atcggcttac ccgtgacctc
     2221 ctcgagctcg gcgataccgg ccgagtcctc gtcgagaagg cgctcgatga cctgaggacg
     2281 cgcgagcacc agcagctcct gaaagtcgaa ttgccggtgc tgacgcagga tttcgcggaa
     2341 gatctcgtgg cacacggttt cggccgtttt cacgaagccg cggccctcac agctaggaca
     2401 ggcctggcac agcacgtgtt ccaggctttc ccggttccgc ttgcgcgtca tctcgaccaa
     2461 gccgagcggc gagacgctca tgacgtggct cttggcgtgg tcgccggcga gccggtccgt
     2521 gagcgtctgg agcacctgcc ggcgatgctc ttcctcggtc atgtcgatga aatcgatgat
     2581 gatgataccc cctaggttcc gcaggcgcag ttgccgcgcg atggccgcgg ccgcctcgag
     2641 gttggtccgg aagatcgtct cctcgaggtt gcggtggccc acgtagccgc ccgtattgac
     2701 gtcgatcgtc gtcatggcct cggtctggtc gaacacgacg tggccgcccg acttcagcgg
     2761 caccttgcga tgcagcgccc ggtcgatctc gtcgtcgatg ccgtagacgt cgaagatcgg
     2821 ccgcggctcc gtatagagct cgatcaggtt gctgagccgc ggcacgaagt ggtcggcgaa
     2881 ttccttgagc ttggcgaagg tcgcctcgga gtcgacccgc acgcgcgcga tgtcggggct
     2941 cgacgagtcg cgcagcacgc gcagcggcag cggcaagtcc tcgtagacga gcccgcccgc
     3001 cggcgtcttc tccgccttct gctcgatcga ctgccagagc ttgtgcagga acatgacgtc
     3061 ggcctgcagc gcctcgcgcg tcgcgccctc ggccgccgtg cgaatgatgt aaccggccgg
     3121 cggcgcctcg gctttgatct cctcgagcgt gctgcgcagc cgctcgcgct cggcttcgtc
     3181 gtcgatgcgc gacgacaccc ctacacctaa gctgttcggc gtcagcacga ggtagcgcga
     3241 cgggatcgcg atgtacgtcg tcaagcgcgc gcccttggtg ccgagcggct ccttcaagac
     3301 ctgcacgagc agctcttgcc cctcgcggac gaattcgcgg atgtcgggct cgacctcgcg
     3361 cgcgggaccg ggctcggcat gcgggttcac ggcgatgtcc gatgcgtgca ggaacgccgt
     3421 gcgctcgagc ccgagctcga cgaacgcggc gttcatgccg ggcagcacgc gcgacacctt
     3481 gcccttgtag atgttgctga cgaggccgac gcggctggcg cgctcgatct gcacctcttg
     3541 cagcacgccg ctatcgacga gcgcggccct gacttctgtc gatgtcgcgt tgatgaggat
     3601 ctcctcgcgc ttcatcaggc cgctcttgcc gcaagccaga aatcgacgcc gcagcgcgtg
     3661 agcaaaccgg ccgtctcgaa cagcggcagc ccgacgacgc cggaatagct tcccttgatc
     3721 tcttcgacga acacggcggc atgcccctga atgccgtagc cgcccgcctt gtcgaacggc
     3781 tcgccggttc gacagtacgc gagccgctcg gccgcggtcg tcgcgcgaaa tctcacttcg
     3841 gtctcgctcg aggcggtctc gaggccgccg gcgtggcgca gcgcgacggc ggtcaagact
     3901 cgatgcgtgc ggccggacaa gcgttcgagc atggcggcgg cctcggcttc ggtctcgggc
     3961 ttgccgagca cctggccgtc gagcacgacg gcggtatcgg cgccgagcac gggccgcaaa
     4021 tcgccgacgg tgactgccca gaccgactcg gctttggcgg tggcgaggcg cgtgacgtag
     4081 gcctctggcg tctcgcgcgg cagctgctcc tcggggattg ccgctgcccg cacggtgaaa
     4141 cgcacgccga tctggcgcag cagctcgctg cggcgcggcg acgcggacgc caggtagacg
     4201 aaatcctttt tcatttcaaa gaactatacg aggtgtgtgc ggcgccgcct agctcttgtt
     4261 cgggcggggc ggccgttgag ggcctccgcc cggggacgcc gcaagtacat ccctgtaggc
     4321 tccttgcgcg
//
LOCUS       DASSNN010000838         2468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1762831, whole genome shotgun sequence.
ACCESSION   DASSNN010000838 DASSNN010000000
VERSION     DASSNN010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762831"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="VFL84_15700"
     CDS             complement(<1..1364)
                     /locus_tag="VFL84_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7610127.1"
                     /translation="MSVVEVRVPELGDFSAVDVIDVHVKPGDRIAAEDPLVTLETDKA
                     TMDVPAPRGGTVVSVALKKGAKVSAGDLVLTLDADETVAPEPKPSTPAKPAASTPAKP
                     AASAAAKPAAPAPAPAPAPAAVAPSPGKRLAVIGAGPGGYTAAFRAADLGLTVTLVER
                     WPTLGGVCLNVGCIPSKALLHAARVIEEAHEMGERGLTFGKPKLDTEKLTAWKSGVVR
                     QLTNGLGALAKQRKVRVVRGTARFKSPNQLEVERDGGTELVDFDQCIIAAGSEPSVLH
                     GLPDDPRIIDSTGALELKALPKRMLVVGGGIIGLEMATVYDALGVKVSVVELTKTLIP
                     GCDRDLVKPLEKRVGGRYESIMLGTRVTKMEAKTSGITVHFEGDQAPAKPQTYGLVLV
                     AVGRSANGRKIAAEKAGVEVDERGLIAVDKQQRTNVPHIFAIGDIVGPPMLAHKATHE
                     GKVAA"
     gene            complement(1361..>2468)
                     /locus_tag="VFL84_15705"
     CDS             complement(1361..>2468)
                     /locus_tag="VFL84_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET7610128.1"
                     /translation="KVSAGDALLVLQPTGAGQPAAAGAAAPSASATPAEAASRAPAAP
                     AKMPAAARPAAPVRSSVGPTPTSFGEAHASPSVRKLARELGVELGRVRGSGAKGRVTA
                     DDVKAFVKEIMQGGGGAASALPKVAAVDFAKYGRVETEPLSRIQKISGPRLHASWVNV
                     PHVTQHDEADITELEEKRATLKEQAQERGIKLTPLAFIVRACALALAEMPLFKASLAQ
                     DGESLVMKHYTHIGFAADTPQGLVVPVIRDADKKDLYEIAKALTTLSERARAGKLQPD
                     DVQGGVFTISSLGGIGGRFFTPIINAPEVAILGVSRSTWQPVYRDLKFVPRLMLPLSL
                     SYDHRVIDGAKAVRFTTRLAEILASPGQLVEAIP"
BASE COUNT          339 a          877 c          848 g          404 t
ORIGIN      
        1 gccgcgacct tgccttcgtg agtcgctttg tgcgcgagca tcggcgggcc gacgatatcg
       61 ccgatcgcga agatatgcgg cacgttcgtg cgttgctgct tgtcgaccgc aatcaggccg
      121 cgctcgtcga cctcgacacc ggctttctcc gccgcgatct tgcgcccgtt cgcgctgcgc
      181 ccgaccgcga cgagcacgag cccgtacgtc tgcggctttg cgggcgcctg atcgccctcg
      241 aaatgcaccg tgatgccgct ggtcttcgcc tccatcttcg tgacgcgtgt cccgagcatg
      301 atggattcgt agcgcccgcc gacgcgcttc tcgagcggct tcacgaggtc gcggtcgcaa
      361 ccggggatca gcgtcttcgt gagctcgacg acggagacct tgacgccgag cgcgtcgtag
      421 acggtggcca tctcgaggcc gatgatgccg ccgccgacga cgagcatgcg tttcggcagc
      481 gccttgagct cgagcgcgcc cgtcgagtcg atgatgcgcg gatcgtcggg cagcccgtgc
      541 aacacgctcg gctcgctgcc cgcggcgatg atgcactgat cgaagtcgac gagctcggtg
      601 ccgccgtcgc gctcgacctc gagctgattc ggcgacttga agcgcgccgt gccgcgcacg
      661 acgcgcacct tgcgctgctt tgcgagcgcg ccgagcccgt tcgtgagctg gcgaacgacg
      721 ccgctcttcc aagccgtcag cttctcggtg tcgagcttcg gcttgccgaa cgtgaggccg
      781 cgctcgccca tctcgtgcgc ttcctcgatc acgcgcgcag catgcagcag cgccttcgac
      841 gggatgcagc cgacgttcaa gcagacgccg ccgagcgtag gccagcgctc gacgagcgtg
      901 acggtgagtc cgagatcggc ggcgcgaaac gcggccgtgt agccgccggg gcccgcgccg
      961 atcacggcga gccgcttgcc gggcgacggc gccacggcag ccggcgcagg cgcgggcgcg
     1021 ggcgcgggcg cggcaggctt tgcggctgcg ctcgccgcag gcttcgcggg tgtgctcgcg
     1081 gcaggcttcg cgggtgtgct cggcttcggt tcgggagcta ccgtctcgtc ggcgtcgagc
     1141 gtcagcacga gatcgcccgc ggagactttc gcgcctttct tcaacgcgac cgagaccacg
     1201 gtgccgccgc gcggcgccgg cacgtccatc gtcgccttgt cggtctcgag cgtgacgagc
     1261 ggatcctcgg ccgcgatccg gtcgccgggc ttgacgtgga cgtcgatgac gtcgaccgcg
     1321 gagaagtcgc cgagctccgg aacccgcacc tcgaccacgc tcacggaatc gcctcgacga
     1381 gctgaccggg gctcgcgagt atctcggcga gacgcgtcgt gaagcgcacg gccttggcgc
     1441 cgtcgatgac gcgatggtcg tacgacagcg acagcggcag catcaaccgc ggcacgaact
     1501 tcagatcgcg atagaccggc tgccacgtgc tgcgcgagac gccgagaatc gcgacttccg
     1561 gagcgttgat gatcggcgtg aagaaccgcc cgccgatgcc gcccaagctc gagatcgtga
     1621 atacgccgcc ctgaacgtcg tccggttgca gcttgcccgc gcgcgctcgc tcgctgagag
     1681 tcgtgagcgc tttcgcgatc tcgtagagat ccttcttgtc ggcatcgcga atcaccggca
     1741 cgacgagccc ctgcggcgta tcggccgcga agccgatgtg cgtgtagtgc ttcatgacga
     1801 ggctctcgcc gtcctgagcc agcgacgcct tgaacagcgg catctcggcg agcgcgagcg
     1861 cgcacgcgcg cacgatgaac gcgagcggcg tcagcttgat gccgcgctcc tgcgcttgct
     1921 ccttcagcgt ggcacgcttc tcctcgagct ccgtgatgtc ggcctcgtcg tgctgcgtga
     1981 cgtgcggcac gttgacccag ctcgcgtgga gccgcgggcc cgagatcttc tggatgcgcg
     2041 acagcggctc tgtctcgacg cggccgtact tcgcgaagtc gacggcggca accttgggca
     2101 acgcgctcgc ggcgccgccg ccgccttgca tgatctcttt gacgaacgcc ttgacgtcgt
     2161 cggccgtgac gcggcctttc gcgcccgagc cgcgaactcg gccgagctcg acgccgagct
     2221 cgcgcgcgag cttgcggacc gacgggctcg catgcgcctc gccgaacgac gtcggcgtcg
     2281 gccctacgct gctgcgcaca ggtgctgcgg gccgcgccgc ggcgggcatc ttggccggcg
     2341 cagcaggcgc acgcgacgcc gcttccgccg gcgtcgcgct cgccgacggt gccgccgcgc
     2401 cggccgcggc cggctgacct gcgccggtcg gctgcagcac gagcaacgca tcgcccgcag
     2461 acaccttg
//
LOCUS       DASSNN010000839         2983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1763787, whole genome shotgun sequence.
ACCESSION   DASSNN010000839 DASSNN010000000
VERSION     DASSNN010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763787"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..579
                     /locus_tag="VFL84_15710"
     CDS             109..579
                     /locus_tag="VFL84_15710"
                     /inference="COORDINATES: protein motif:HMM:NF018552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HET7610129.1"
                     /translation="MLVDSLLVPQCVYRPGTFTGLMTLYESNYIKLHQLASSMKWPAD
                     AVHSSSPRDNDLHAEIVRREPYTTTLRLTYWFEEAGVPAPDPDLVVRIYHDARLVEAV
                     SSGDTHRHMKLRELAKSGSAELDRRWRLNMLLNKWLDYLVEVGHSLDFEGRALS"
     gene            682..1722
                     /locus_tag="VFL84_15715"
     CDS             682..1722
                     /locus_tag="VFL84_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7610130.1"
                     /translation="MLSAIRERQQPGDELLKLYWNRADVKRELKALRRERHELLDKLK
                     EQEGAILRAQEQLEGLERLLTNPLAAANAMVYFQLRHLWRVGAQRLEQFGRELQTQRE
                     KRERAQLHDAEVAKRERRLEAVKEKLASIGEKSRTAAEEQARLEQALASMNGFVRLFR
                     SGGLKRQIAGVASGRKVLEEKLEELQALTEKIQGEPLPEPEELSLDSRRLINTAIIAL
                     AQHLVVHFSEHDLASLAKTSTERAVADMKFGDRRDCDRMVERIREKIEELKQQKTFAD
                     QVKRRTDLLLTEVKYRNETDSVPAIESVQLTPRMPAAQGDEVDLARRASDAPLHINVV
                     ADDYWDLLAVMR"
     gene            complement(1727..>2983)
                     /locus_tag="VFL84_15720"
     CDS             complement(1727..>2983)
                     /locus_tag="VFL84_15720"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HET7610131.1"
                     /translation="PAQIDGQLKQEDRITAVGEGSGELVDVIGWRLDDVVQKIRGPGG
                     SRVRLQVLPAGAAPGSPEKIIELVRDKIKLEEQAAKKELLQIPFDGKPYRIGVITVPS
                     FYQDFAARSRGDDDYTSTSRDVARLIEEFRAEGGIDGLVMDLRANGGGHLSEATELSG
                     LFIERGPIVQLRETRGTIQELPDPSPNAVYNGPLAVLVDRYSASASEIFAAAIQDYDR
                     GVIVGQQTFGKGSVQNLFPLDRLMRGTDNGQLTLTIGKYYRVTGESTQHRGVIPDIEL
                     PSMVDASTVGESTRDTALPWDRIQPTRFRADPALAGRIATLRAHQQVRAAEDPEFRYL
                     LSDIAAVKEIAAQKTISLNLNGRVAENKRLEEGRLARENARRTALGQEPLTSIDQLDD
                     TATSDAILLHQAARIVAEIAEGRPAQ"
BASE COUNT          523 a          956 c          999 g          505 t
ORIGIN      
        1 tgctggccgg catcgtgatc gggctcgtcg cgcccacgtt gcgacgcaag cggcgttccg
       61 attggtagcg ggttgcctga aaactgactt taggccactg tagcaaccat gctcgtcgac
      121 agcttactgg ttccacaatg cgtctaccgc cccggcacgt tcaccgggct catgacgctg
      181 tacgaaagca actacatcaa gctgcaccag ctggcgtcgt cgatgaaatg gcccgccgac
      241 gcggttcact cgtcttcgcc gcgagacaac gatctgcacg ccgagatcgt gcgccgcgag
      301 ccgtatacga cgacgctgcg cctcacgtac tggttcgaag aggcgggcgt gccggcgccg
      361 gatcccgacc tcgtcgtgcg catttaccac gatgcgcggc tcgtcgaagc cgtcagcagc
      421 ggcgacactc atcggcacat gaagctgcgc gagctcgcca agagcggcag cgccgagctc
      481 gatcggcgct ggcggctcaa catgctgctg aacaagtggc tggactacct ggttgaagtc
      541 ggtcacagcc tcgatttcga ggggcgcgct ctgagctgaa cgctcgggaa gcctcgttcc
      601 ttgctttgag tcacggttta gcgaggaggt cgcggatata tttcggggca tgccgtcgta
      661 ttgcaaaacg aaggagctct gatgctctcg gccattcgag agcggcagca accgggcgac
      721 gagctgctga agctctattg gaaccgggcg gacgtcaagc gtgagctcaa ggcgcttcgg
      781 cgcgagcgcc acgagctcct ggacaagctc aaagagcaag agggcgcgat tttgcgcgcc
      841 caggagcagc tcgaaggcct cgagcgcctc ctgacgaatc cgttggcggc cgccaacgcg
      901 atggtctact tccagcttcg ccacctttgg cgcgtcggcg cgcaacggct cgagcagttt
      961 gggcgcgagc tgcagacgca gcgcgagaaa cgcgaacgcg cgcagctcca cgacgcggag
     1021 gtggcgaaac gcgagcggcg gctcgaggcg gtcaaggaga agctcgccag catcggcgag
     1081 aagagccgca ccgcggccga ggagcaagcg cggctcgagc aagcgctcgc gagcatgaac
     1141 ggcttcgtgc gcctgtttcg ctcgggcggc ctcaaacggc agatcgccgg tgtcgcgagc
     1201 ggccgcaagg tgctcgagga aaagctcgag gagctgcagg ccctgaccga gaagatccaa
     1261 ggcgagccgt tgccggagcc ggaggagcta tcgctcgaca gccggcgcct gatcaacacg
     1321 gcgatcatcg cgctcgctca gcacctcgtg gtccatttct cggagcacga cttggcgtca
     1381 ctggcgaaaa cgtcgacgga acgcgccgtg gccgacatga agttcggcga tcgccgcgat
     1441 tgcgaccgca tggtcgagcg catccgcgaa aagatcgaag agctcaagca gcagaagact
     1501 ttcgcggacc aggtcaaacg gcggacggac ttgctgctca ccgaggtcaa gtaccgcaac
     1561 gagacggatt ccgtgccggc catcgaaagc gtgcagctga cgccacgcat gccggccgcg
     1621 caaggcgacg aggtcgacct cgcgaggcgc gccagcgacg cgccattgca catcaacgtc
     1681 gtcgccgacg actattggga tctgctcgcc gtaatgcgct gagccgctac tgcgccgggc
     1741 gtccttcggc gatctcggcg acgatccgcg ccgcttgatg cagcaggatt gcgtccgagg
     1801 tcgcggtatc gtcgagctga tcgatgctcg tcagcggctc ctgtccgagc gccgttcggc
     1861 gcgcgttctc gcgcgccagc cggccctcct cgaggcgctt gttctccgcg acgcggccgt
     1921 tcaaattcag cgagatggtc ttctgcgcgg cgatctcctt gacggcggcg atgtcgctca
     1981 gcagatagcg gaactcgggg tcttcggcgg cgcgtacctg ctggtgcgcg cgcaaggtgg
     2041 cgatccgacc cgcgagcgca gggtcggcgc ggaaacgcgt cggctgaatg cggtcccacg
     2101 gcaaagccgt gtcgcgtgtg ctctcgccta cggtggacgc atcgaccatc gacggcagct
     2161 cgatgtcggg aatgacgccg cgatgctgcg tgctttcacc cgtgacgcga tagtacttgc
     2221 cgatcgtcag cgtgagctga ccgttgtcgg tgccgcgcat gaggcggtcg agcgggaaca
     2281 gattctggac cgagcctttg ccgaacgtct gctggccgac gatgacgccg cggtcgtagt
     2341 cctgaatcgc ggccgcaaaa atctccgacg cgctggcgct atagcgatcg acgagcacgg
     2401 ccaagggacc gttgtagacg gcgttgggac tgggatcggg cagctcctga atcgtgccgc
     2461 gcgtctcgcg cagctgaacg atcgggccgc gctcgatgaa caggccggaa agctccgtgg
     2521 cctccgacag atggccgccg ccgttggcgc gcaggtccat gacgaggccg tcgatgccgc
     2581 cttcggctct gaattcctcg atgagccgcg cgacgtcgcg cgacgtgctc gtgtagtcgt
     2641 cgtcgccgcg gctgcgcgcc gcgaagtcct gatagaagct cggaacggtg atgacgccga
     2701 tccgatacgg cttgccgtcg aacgggatct gcagcagctc cttcttcgcc gcctgctcct
     2761 cgagcttgat cttgtcgcgc acgagctcga tgattttttc cggcgagccc ggtgccgcgc
     2821 cggcgggtag gacctgcagc cgaacgcgcg agccgccggg gccgcgaatc ttttgcacga
     2881 cgtcgtcgag acgccagccg atcacgtcga cgagctcgcc gctgccttcg ccgaccgccg
     2941 tgatgcggtc ctcttgcttc agctgcccgt cgatctgcgc ggg
//
LOCUS       DASSNN010000840         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_749406, whole genome shotgun sequence.
ACCESSION   DASSNN010000840 DASSNN010000000
VERSION     DASSNN010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749406"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /gene="aroA"
                     /locus_tag="VFL84_15725"
     CDS             <1..1064
                     /gene="aroA"
                     /locus_tag="VFL84_15725"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET7610132.1"
                     /translation="ALDVGNSGTAIRLLMGLLAGQSFDSTLTGDASLKQRPMERVAAP
                     LRQMGARITTSDGGRPPVAIKGGASLHGVDYELPMASAQVKSALLLAGLNASGATTVR
                     SPGPSRDHTERMLKSMGVRLTLAAEGAGHRVTLSGPVTLRGGEVLVPADFSSAAFFIV
                     AACLAARDGLTIENVGINPTRTGLLTILREMGARIELRNERSLGAEPVADLWVEQSEL
                     RGIVVDPSLVPLAIDEFPILFVAAAAAKGETVVSGADELRKKETDRIAVMARALRAVG
                     VEVEERPDGARIVGGRMRGGTVDSRGDHRIAMSFAVASLRASGPIDILSTAEVATSFP
                     SFLEVASAAGLDVRAEERA"
     gene            1061..1762
                     /gene="cmk"
                     /locus_tag="VFL84_15730"
     CDS             1061..1762
                     /gene="cmk"
                     /locus_tag="VFL84_15730"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015487405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET7610133.1"
                     /translation="MTRRAVPVVTIDGPSGSGKGTIGRLLAQKLGWHYLDSGALYRLV
                     ALAALRRHLDFRDAAALAALAANLDVRFTPAAAGDRVYLEGADVSSELRTERAGDAAS
                     KVAAIPEVRAALLKRQRDFAVPPGLVADGRDMGTVVFPDAVLKVVLTASAEARAMRRH
                     KQLKEKGIDVSLPDLSWDIAQRDARDANRTVAPFKPAPDAHVIDSTSLTPEEVVAHIL
                     NRLEDVGVVPERAKG"
     gene            1791..3515
                     /gene="rpsA"
                     /locus_tag="VFL84_15735"
     CDS             1791..3515
                     /gene="rpsA"
                     /locus_tag="VFL84_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018935518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET7610134.1"
                     /translation="MNPPEQDARRNAEGIMSESFAQLLEESFASQKIKTGAILTGVVV
                     GVNADMVIVNAGLKSEAVIPADQFFNERGELEVKVGDHVEVALDTVEDGFGETRLSRE
                     KAKRTRTWTRLEEAFQKGEIVKGTISGRVRGGFTVDIDLVRAFLPGSLVDVRPIRDPA
                     ALEGSTLEFKVIKLDQKRNNVVVSRRAVVEAEYSAERESLLKNLQEGTTLKGVVKNLT
                     DYGAFVDLGGIDGLLHITDMAWKRVKHPSEVVSVGQELEVKVLKFDRERNRVSLGLKQ
                     LGADPWADLARRYPPGTRLFGKVTNIADYGCFAEIEEGVEGLVHVSEMDWTNKNVNPH
                     KVVQIGDEVEVMVLDIDEERRRISLGLKQCKPNPWVEFGENYKKGDKVSGQIKSITDF
                     GVFIGLPGGIDGLVHLSDLSWDVPGEEAVRNYKKGEELETTVLAIDPERERISLGVKQ
                     LDKDPLSLFLGDHPKGSIVSGVVQELDAKLATVAVEGGILGHLRASEIARDRVEDART
                     ALKVGDKIEAKITGVDRKTRIISLSIKAKEAHEEQQALQNYKSESGRTTTGTTLGDLL
                     KEQMNPNE"
     gene            3538..3873
                     /locus_tag="VFL84_15740"
     CDS             3538..3873
                     /locus_tag="VFL84_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007963243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HET7610135.1"
                     /translation="MCRGRATRSVPMTKSELIESIARSQKHLPAKDVELAVKHILELM
                     SEALANGERIEIRGFGSFSLHYRPPRMGRNPKTGEAVALAGKNVPHFKPGKDLRERVN
                     ESRHLPVRD"
BASE COUNT          701 a         1247 c         1345 g          595 t
ORIGIN      
        1 ccgcgctcga tgtcggcaat tccggcaccg cgattcgcct gctgatggga ttgctcgcgg
       61 gccaatcgtt cgacagcacg ctgaccggcg acgcgtcgct gaagcagcgg ccgatggagc
      121 gcgtcgcggc gccgctgcgg cagatgggcg cgcgaatcac gacgtcggac ggcggccggc
      181 cgcccgtcgc gatcaagggc ggcgcctcgt tgcacggcgt cgactacgag ttgccgatgg
      241 cgagcgcgca ggtcaaatcg gcgctgctgc tcgcgggcct caatgcgagc ggagcgacga
      301 cggtgcgctc gccggggccg agccgcgacc atacggagcg aatgcttaag agcatgggcg
      361 tgcgcctcac gttggcggcc gaaggcgccg ggcatcgcgt cacgctttca ggaccggtca
      421 cgctgcgcgg cggcgaagtg ctcgtgcccg cggatttcag ctcggccgcg ttcttcatcg
      481 tcgcggcttg cctcgcggcg cgcgacgggc tcacgatcga gaacgtcggt atcaatccga
      541 cgcgcaccgg cctgctcacg atcctgcgcg agatgggcgc gcgcatcgag ctgcgcaacg
      601 agcgcagctt gggagcggag cccgtcgccg acctctgggt cgagcagagc gagctgcgcg
      661 gcatcgtcgt tgatccttcg ctcgtgccgc tcgcgatcga cgagtttccg atcctattcg
      721 tcgcggccgc ggcggcgaag ggcgagaccg tcgtcagcgg cgccgacgag ctgcgcaaga
      781 aagagaccga ccgcattgcc gtgatggccc gcgcgcttcg cgccgtcggc gtcgaagtcg
      841 aggaacggcc cgacggcgcg cgcatcgtcg gcgggcgcat gcgcggcggc accgtcgaca
      901 gccgcggcga tcaccgcatc gcgatgtcgt tcgcggtcgc gagcctgcgc gccagcggcc
      961 cgatcgacat cctgagcacg gccgaggtcg cgacgtcgtt cccgagcttc ctcgaggtcg
     1021 cgtccgccgc cggtctcgac gtgcgtgccg aggaacgcgc gtgacgcgcc gggcggttcc
     1081 cgtcgtcacg atcgacggcc cgagcgggtc gggcaagggc acgatcggcc ggctgctcgc
     1141 gcagaagctc ggctggcact atctcgacag cggcgcgctc tataggctcg tcgcgctcgc
     1201 ggcgttgcgc cggcacctcg attttcgcga cgcggcggcg ctggcggcgc tcgcggcgaa
     1261 cctggacgtc cgcttcacgc cggccgcggc cggcgaccgc gtatacctcg aaggggccga
     1321 cgtcagctcg gagctgcgca cggagcgggc cggcgacgcg gcctcgaaag tcgcggccat
     1381 cccggaggtc cgtgccgcgc tgctgaagcg ccagcgcgat ttcgccgtcc cgccgggcct
     1441 cgtcgcggac gggcgcgaca tgggcacggt cgtcttcccg gacgccgtac tcaaggtcgt
     1501 gctgacggcc agcgccgagg cgcgcgccat gaggcgtcat aagcagttga aagaaaaggg
     1561 gattgatgtt agcctgcccg acctttcctg ggacatagcg caacgggacg cgcgcgacgc
     1621 gaaccgcacg gtggcgcctt tcaagccggc accggatgcg cacgtcatcg actccacgag
     1681 tctgacgccc gaggaagtcg tggcccacat cttgaatagg ctcgaggacg tgggcgtcgt
     1741 accggaacga gccaaagggt aagcgggggc aggatgccgc cgttcgtcga atcaacccgc
     1801 cggagcagga tgccaggcgg aatgcggaag gaatcatgag cgaatcgttt gcacaactgc
     1861 tggaagagag tttcgcgagt cagaaaatca agaccggtgc cattctcacc ggtgtagtcg
     1921 tcggcgtgaa tgccgacatg gtgatcgtca atgccgggct gaagtccgag gcggtcatcc
     1981 cagccgatca gtttttcaac gagcgcggcg agctcgaagt caaagtcggc gaccacgtcg
     2041 aagtggcgct cgacaccgtc gaggacggct tcggcgagac gcgcttgtcg cgcgagaagg
     2101 ccaagcgcac gcgcacctgg actcgcctcg aggaggcgtt ccagaaaggc gaaatcgtca
     2161 agggcacgat cagcggccgc gttcgcggcg gcttcaccgt cgacatcgat ttggtccgag
     2221 cgttcttgcc gggctctctc gtcgacgtgc gcccgatccg cgaccccgcg gcgctcgaag
     2281 gctcgacgct cgagttcaag gtcatcaagc tcgatcagaa gcgcaacaac gtcgtcgtct
     2341 cgcgccgcgc ggtcgtcgag gccgagtaca gcgccgagcg tgagtcgctg ctcaagaacc
     2401 tccaggaagg cacgacgctg aagggcgtcg tcaagaacct caccgattac ggcgcgttcg
     2461 tcgacctggg cggcatcgac ggcctgctgc acatcacgga catggcgtgg aagcgcgtca
     2521 agcatccgtc cgaggtcgtc agcgtcggcc aagagctcga ggtcaaggtg ctgaagttcg
     2581 accgcgagcg caatcgcgtc tcgctgggct tgaagcagct cggcgccgat ccctgggccg
     2641 atctcgcgcg gcgttatccg ccgggcacgc ggctcttcgg caaggtcacg aacattgccg
     2701 actacggctg cttcgccgag atcgaggaag gcgtcgaagg cttggtccac gtgtccgaga
     2761 tggactggac caacaagaac gtcaacccgc acaaggtcgt gcaaatcggc gacgaggtcg
     2821 aggtcatggt cctcgacatc gacgaggagc gccgccgcat ctcgctcgga ctcaagcagt
     2881 gcaagccgaa cccgtgggtc gagttcggcg agaactacaa gaagggcgac aaggtttccg
     2941 gtcagatcaa gtcgatcacg gatttcggcg tcttcatcgg cttgccgggc ggcatcgacg
     3001 gcctcgtgca tctctcggat ctctcttggg acgtgcccgg cgaagaagcc gtgcgcaact
     3061 acaagaaggg cgaggagctc gagacgacgg tgctcgcgat cgaccccgag cgcgagcgca
     3121 tctcgctcgg cgtcaagcag ctcgacaagg atccgctgtc gctgttcctg ggcgaccacc
     3181 cgaagggcag catcgtgtcc ggcgtcgtgc aagagctcga cgcgaagctc gcgacggtcg
     3241 ctgtcgaggg cggcatcctg ggccatctgc gtgcgtccga gatcgcgcgt gatcgcgtcg
     3301 aggacgcgcg cacggcgctc aaggtcggcg acaagatcga ggcgaagatc acgggcgtcg
     3361 accgcaagac gcgcatcatc tcgctgtcga tcaaggccaa ggaggctcat gaggagcagc
     3421 aagcgttgca gaactacaag agcgagtccg gtcgcacgac gacgggcacc acgctcggcg
     3481 acttgctgaa agagcagatg aatccgaatg agtgagggct tcgcgcatta gcgcgaggtg
     3541 tgccgaggtc gagcaacaag gtccgtaccg atgacaaaat ccgagctgat cgagtcgatc
     3601 gcgcgcagtc agaagcattt gcctgccaag gacgttgagc tggccgtcaa gcacattctc
     3661 gagctcatga gcgaggcgct ggcgaacggc gaacgaatcg aaatccgcgg tttcggttcg
     3721 ttctctttgc actaccgccc gccgcggatg ggtcgcaacc ccaaaaccgg cgaggcggta
     3781 gcgctcgcgg gaaagaacgt cccgcacttc aaacccggca aggaccttcg cgagcgagtc
     3841 aacgagtcgc ggcacctgcc cgtgcgtgac tagcgcctac ggaggtgc
//
LOCUS       DASSNN010000841         4495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_1386391, whole genome shotgun sequence.
ACCESSION   DASSNN010000841 DASSNN010000000
VERSION     DASSNN010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386391"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFL84_15745"
     CDS             complement(<1..368)
                     /locus_tag="VFL84_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HET7610136.1"
                     /translation="MKRRLIRGAAFCACLGALAARAAEPIPVLILDGESGGPYHDWPR
                     VTAVLERVLGEVGMFQVSVATAPPEGGDFTKFAPNFAAFKAVVLNYDAPDERWPAALK
                     SSLERYVENGGGMVSVHAAD"
     gene            428..1267
                     /locus_tag="VFL84_15750"
     CDS             428..1267
                     /locus_tag="VFL84_15750"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7610137.1"
                     /translation="MQPPSLRRATLFAAAALLCASNALAQPPRSEESPAARPVPPSAT
                     PQSYTAELVAAGRTRFGASCGFCHGPDATGGSGGADLTRSEIVAADVRGDRIGPLVRT
                     GRAEAGMPAFPALAENDLAAIVAFIHEQKAKAEAEVGGRRSVEVADVLTGNARAGRRY
                     FESACASCHSATGDLAGIASRLEGLALLRRMLYPGGQAGGGRRPTATVTTQGGETIAG
                     EVAYRDEFTLAVIDGGGRYRSWPADRVRFTIDDPLEAHVAQLARYTDATMHDVLAYLH
                     TLR"
     gene            1340..2881
                     /locus_tag="VFL84_15755"
     CDS             1340..2881
                     /locus_tag="VFL84_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acido-empty-quinoprotein group A"
                     /protein_id="HET7610138.1"
                     /translation="MALAALVSAAHGQTGTVGGELDSFSLLRPPQDSWPTYHGDYSGR
                     RHSALTEITQDNVHTLALQWAFQTNQAQQIKATPILVDGIVYLTAPDNLWAIDARSGR
                     QIWRYQHPANDAFHIGHRGAAVHGDSVFLTTPDAHLLAFDRFSGKLKWNVEVADSSRG
                     FWSTNAPLVIGEHVIVGVSGDFDNLPGTLRAFDAATGAAQWTFYSTPPAGTPDSLAGG
                     ATGGQMWMTGTYDPALDLLYVGTGNPTPVLNGATRPGDNKWTCSIVALDPDTGKLKWG
                     FQVSPHDTHDWDASEVPVLVDAKLNGADRKLLLQASRNGYYVVLDRVTGENLLTTPFA
                     AVNWSLGVDGKGQPIPNPAKEPARDGRLVAPDELGATNYRSPSFDPATGLFIVSAADA
                     YGVYFFKPEHGEYGWAGADYGVHSRGELRALDFATGEVRWRHDLFGAAGSAGVLTTAS
                     GLTFTGDSANSALALRTSDGATLWHAGVGRVGNAPVTYELDGRQYVLIGGGSALYAFA
                     LPEPR"
     gene            2897..4348
                     /gene="xylB"
                     /locus_tag="VFL84_15760"
     CDS             2897..4348
                     /gene="xylB"
                     /locus_tag="VFL84_15760"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HET7610139.1"
                     /translation="MYLGVDLGTSAVKATLIDGEQSIVGSAAAPLEVSRPRPGWSEQD
                     PRDWLTAAERAIDALRAAHERELKAVRGIGLSGQMHGATLLDERDEPLRACMLWNDTR
                     AHAEAAALDGDARFRAVTGNIVFPGFTAPKVAWVRKHEPRVFERLRRVLLPKDYLRLW
                     LTGEHVSEMSDASGTSWLDTGARRWSHELLAATELEARQMPALIEGTAASGTLRRELA
                     ARWGMAGAVVVAGGAGDNAAAACGLGAVRDGEAFVSLGTSGVLFAATRAHSPNAASAV
                     HAFCHALPDTWHQMGVILSATDSLNWLANLTGTSAAELTASLGERLAAPGRVTFLPYL
                     GGERTPHNDANVRGVFVGLGHESDRRALAQAVVEGVAFALCDSCDALRAAGTHIARLK
                     AVGGGTRSRYWLQVLATALGVTVDVPAAGDFGAAFGAARLGLIAATGAKPGRVCLAPK
                     VAETIEPNAALAPAFAAARERYRALYPAIRGAR"
BASE COUNT          651 a         1607 c         1587 g          650 t
ORIGIN      
        1 ttgtccgcgg cgtgcacgct caccatgccg ccgccgttct cgacgtagcg ttcgagcgag
       61 gacttcaacg ccgccggcca gcgctcgtcg ggcgcgtcgt agttcagcac gacggccttg
      121 aacgctgcga aattgggcgc gaacttcgtg aagtcgccgc cctcgggcgg cgccgtcgct
      181 acgctgactt ggaacatgcc gacctcgccg agcacgcgct cgagcactgc cgtcacgcgc
      241 ggccaatcgt gatagggacc gccgctctcg ccgtccaaga tcaagacggg aatgggctcg
      301 gccgctcgcg cagcgagcgc gcctaagcat gcgcagaacg cggcgcccct gatcagccgg
      361 cgtttcatgg ctggtcatga tcgcacgagt tgacgctatt gaaagcgcca tgttaactct
      421 ccgcgccatg cagcccccat cgctccgccg cgccaccctc ttcgccgcgg ccgcgttgct
      481 ctgcgcctcg aacgcgctcg cacaaccgcc tcgcagcgaa gagagccccg ccgcgcggcc
      541 ggtcccgcct tcggcgacgc cgcagtcgta cacggccgag ctcgtcgcgg cgggccggac
      601 gcggttcggg gcaagctgcg ggttctgcca cggacccgac gcgacgggcg gctcgggcgg
      661 cgctgatttg acgcgctcgg agatcgtggc ggccgacgtg cgcggcgacc ggatcggacc
      721 gctcgtgcgc accgggcggg ccgaggccgg tatgccggcg tttccggcgc tcgcggaaaa
      781 cgatctcgcc gcgatcgtgg cgttcatcca cgagcagaag gcgaaggcgg aagccgaggt
      841 cggcggccgc cgctcggtcg aggtcgccga cgtgctgacg ggaaacgcca gggccgggcg
      901 gcgctatttc gagtcggcct gcgcgagctg tcactcggcg accggcgacc tcgccggcat
      961 tgcgtcacga ctggagggcc tcgcgctgct ccgacgaatg ctctatccgg gcggccaggc
     1021 cggcggaggg cgccgcccta ccgcgaccgt gacgacgcag ggcggcgaga cgatcgccgg
     1081 cgaagtcgcg taccgcgacg agttcacgct cgccgtgatc gacggcggcg gccgctaccg
     1141 ttcctggccg gcggatcgcg tgcgcttcac gatcgacgac ccgctcgagg cccacgtcgc
     1201 gcagctcgcg cgctacacgg acgcgacgat gcacgacgtc ctcgcctacc tgcacacgct
     1261 tcgttaggac accgccatgc agcatgcatc cgcgcccaga tcgcgagcga ctcttcgatg
     1321 cgggcgcgcg ctcgcgtgcg tggcgctcgc ggcgctcgtc agcgcggcgc acggccaaac
     1381 cgggacggtc ggcggcgagc tcgattcctt ctcgctgctc cgcccgccgc aagacagctg
     1441 gccgacctac cacggcgact actcgggccg gcgccacagt gcgctgacag agatcacgca
     1501 agacaacgtc cacacgctcg cgctgcagtg ggcgttccag acgaaccagg cgcagcagat
     1561 caaagcgacg ccgatcctcg tcgacggcat cgtctacttg acggccccgg acaatctctg
     1621 ggccatcgac gcgcgctcgg gacggcagat ctggcgctat cagcatcccg cgaacgacgc
     1681 attccacatc ggccatcgcg gcgccgccgt gcacggcgac tccgtattcc tgacgacgcc
     1741 cgacgcgcac ttgctcgcct tcgaccgctt cagcggcaag ctgaagtgga acgtcgaggt
     1801 cgcggactca tctcgcgggt tctggtcgac gaatgcgccg ctcgtgatcg gcgagcacgt
     1861 catcgtcgga gtctcgggcg acttcgacaa cctgccgggc acgttgcgcg cgttcgatgc
     1921 ggcgacgggt gcggcgcagt ggacgttcta cagcacgccg ccggccggca cgcccgactc
     1981 gctcgcgggc ggcgcgaccg gcgggcagat gtggatgacg ggcacgtacg accccgcgct
     2041 cgatctgctc tacgtcggca ccggcaatcc gacgcccgtg ctgaacggcg cgacgcggcc
     2101 cggcgacaac aagtggacgt gcagcatcgt cgcgctcgat cccgatacgg ggaagcttaa
     2161 gtggggcttt caggtgtcgc cgcacgacac gcacgactgg gacgcctccg aggtacccgt
     2221 gctcgtcgac gcgaagttga acggcgccga tcgcaagctg ctgctgcagg cctcgcgcaa
     2281 cggttactac gtcgtgctcg accgcgtgac cggcgagaat ctcctgacga caccgttcgc
     2341 ggccgtcaac tggtcgctcg gcgtcgacgg caagggccaa ccgatcccga atccggccaa
     2401 ggagcccgcg cgcgacgggc gcttggtcgc acccgacgag ctcggcgcga cgaactatcg
     2461 atctccgagc ttcgaccccg cgacgggctt gttcatcgtc agcgccgcgg acgcgtatgg
     2521 cgtctacttc ttcaagcccg agcacggcga atacggctgg gcgggcgccg actacggcgt
     2581 tcacagccgc ggcgagctgc gcgcgctcga cttcgccacg ggcgaggtgc gctggcgcca
     2641 cgatctcttc ggcgccgcgg gctcggcggg cgtgctgacg acggcgtccg gtctcacgtt
     2701 cacgggcgac tccgcgaata gcgcgctggc gcttcgcacg agcgacggcg cgacgctctg
     2761 gcacgccggc gtcgggcgcg tcggcaacgc gcccgtcacc tacgagctcg acgggcggca
     2821 gtacgtgctg atcggcggcg gcagcgcgct ttacgcgttc gcgctgcctg agccgcgcta
     2881 gccgcgcgcg ccgccgatgt acctcggcgt cgatctcggc acgtcggccg tcaaagcaac
     2941 gttgatcgac ggcgagcaga gcatcgtcgg ctccgcggcc gcgccgctcg aagtgtcgcg
     3001 gccgcggccg gggtggagcg agcaagatcc gcgcgattgg cttacggccg ccgagcgcgc
     3061 gatcgacgcc ttgcgcgcgg cacatgagcg cgagctcaag gccgtgcgcg gcatcgggct
     3121 ctcgggccag atgcacggcg cgacgctgct cgacgaacgc gacgagccgc ttagggcctg
     3181 catgctctgg aacgacacgc gcgcgcacgc cgaagccgcg gcgctcgacg gcgacgcacg
     3241 cttccgcgcc gtcacgggca acatcgtctt ccccggtttc acggccccga aagtcgcgtg
     3301 ggtgcgcaag cacgagccgc gcgtgttcga gcgcttgcgg cgcgtgctgc tgccgaagga
     3361 ctacctgcgc ctctggctca cgggcgagca cgtgtccgag atgtcggacg cgtcggggac
     3421 gagctggctc gacacgggcg cgcgccgctg gtcgcacgag ctgctcgcgg cgacggagct
     3481 cgaagcgcgg caaatgccgg cgctgataga agggaccgct gcgagcggca cgctgcgccg
     3541 cgagctcgcg gcgcgctggg gcatggccgg cgcggtcgtc gtcgccggcg gcgccggcga
     3601 caacgcggcc gcggcgtgcg gtctcggcgc ggtgcgcgac ggcgaagcgt tcgtctcgct
     3661 cggcacgtcg ggcgtgctgt ttgcggcgac gcgtgctcac tcaccgaacg ccgcgagcgc
     3721 cgtgcacgcg ttctgtcacg cgctgccgga cacctggcat cagatgggcg tgatcctctc
     3781 ggccaccgac tcgctgaact ggctcgcgaa tttgacgggc acgagcgcag cagagctcac
     3841 ggcatcgctc ggcgagcggc tcgcggcgcc gggccgcgtg acgttcctgc cgtacctggg
     3901 cggcgagcgc acgccgcaca acgacgcgaa cgtgcgcggc gtgttcgttg ggctcgggca
     3961 cgagtccgac cggcgcgcgc tcgcacaggc cgtcgtcgag ggcgtcgcgt tcgcgctctg
     4021 cgacagctgc gacgcgttgc gagcggcggg tacgcacatc gcgcggctca aggccgtcgg
     4081 cggcggcacc cgctcgcgct attggctgca agtgctcgcc acggcgctcg gcgtgacggt
     4141 cgacgtgccg gccgccggcg acttcggcgc ggcgttcggg gcggcgcggc tcggtctgat
     4201 cgcggcgacg ggcgcgaagc ccggtcgcgt gtgcctggcg ccgaaggtcg ccgagacaat
     4261 cgagccgaac gccgcgcttg cgccggcgtt cgcggccgcg cgcgagcgtt atcgagcgct
     4321 ctatcccgcg atccgcggcg cgcgctaagt ctcgcgcgac caacgcggtt ctctaccacc
     4381 cgcaccgact cggcgctcat acaaggtcaa gcgtatttct gcgctacatc gccggtttgt
     4441 gccctcgccg ggggcgcatc gagcttgcaa cgctaagcgg atgatgggcc gattt
//
LOCUS       DASSNN010000842         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1226
            k141_754908, whole genome shotgun sequence.
ACCESSION   DASSNN010000842 DASSNN010000000
VERSION     DASSNN010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963381
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:01:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,155
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,115
            CDSs (with protein)               :: 3,115
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754908"
                     /isolate="SMAG_U1226"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..730)
                     /gene="ccmB"
                     /locus_tag="VFL84_15765"
     CDS             complement(2..730)
                     /gene="ccmB"
                     /locus_tag="VFL84_15765"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HET7610140.1"
                     /translation="MSAPAPATFAEPQDADETQSVLAAFGWALARDVRIALRSKAELG
                     VQLLFYVIVVSLFPLAASPSRDVLGTLGPGVLWTAALLASLLSLPRLFTSDFADGTLE
                     QIALSPRPLTALVSGKIAAHWLTTGLPVALMAPVLGAQYALDSALLGMLSLSLLIGTP
                     ILSLLGAVGAALTLGARGGGSLLALLVLPLYVPVLIFGAGAVDSVRTGVAAGAHLSLL
                     GAGLLMAAVAAPLAAAAAVRIALD"
     gene            complement(727..1434)
                     /gene="ccmA"
                     /locus_tag="VFL84_15770"
     CDS             complement(727..1434)
                     /gene="ccmA"
                     /locus_tag="VFL84_15770"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HET7610141.1"
                     /translation="MPEPSGRRASPSPLTEQRDASLEGDRLCAQRGYATLFAEVSFAV
                     RDGSALVVTGRNGSGKTTLLRMLAGFTAPAAGEIRWRRSATRPFDARLRAAVAFNGHL
                     PALKDELTAEENLAQWISLGDRPAASASIRDALAAVALERRRSLPVRLLSQGQRRRIG
                     LARLHLSRRPLWILDEPLTALDSDGIDVLRNLLDAHLEAGGICVAASHQPLPVALERQ
                     QSLRLEGSGSAPMERDP"
     gene            complement(1452..2681)
                     /gene="ccmI"
                     /locus_tag="VFL84_15775"
     CDS             complement(1452..2681)
                     /gene="ccmI"
                     /locus_tag="VFL84_15775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HET7610142.1"
                     /translation="MPSAALFWLVAIALCAATVAALVWPLLRARGPRSDASEDSATTG
                     VYRDQKRQLDAELAAGAITADEHDVALRELSGRLAQELQSGEPAAAPASPARSSYVAA
                     LVVAAALPVTALALYAAFGNPEAMRVAGSADASAPMSHEQIESMVERLALRMKEKPDD
                     PTGWRLLARAYSAMGRYDDAVAAFTQAAIRGTEDAPLLADWADALAMKNQSLQGEPSR
                     LVARALALDPNHPKALALAASAAMDRHDYDAAIAEWKKLKGQFPQDSAEVKEIDAMIA
                     EAGAAKGGASATASGATDAAAAITGSVSLDPKLRSRTAASDTLFIYARSAQGPRMPLA
                     IVRTTAGEWPRTFRLDDSMAMTPATKLSSANEVVVEARVSKTGNATPSPGDLHGASAA
                     VKPGARDVLIVINDVVP"
BASE COUNT          444 a          972 c          970 g          383 t
ORIGIN      
        1 cctaatcgag cgcgatgcgc accgccgcgg cagcagcaag cggtgcggcg acggcggcca
       61 tcagcaatcc cgccccgagc agtgaaaggt gcgcgcctgc cgcgacgccc gtgcgcaccg
      121 aatcgacggc gcccgcgccg aaaatcagca ccgggacgta cagcggcaac accagcagcg
      181 ccaggaggct gccgccgccc cgtgcgccaa gcgtcagcgc cgcgccgact gcgccgagga
      241 gcgaaaggat cggcgtgccg atcagaagcg acagcgacag catgccgagc agcgccgaat
      301 cgagcgcgta ttgcgcgccg agcaccggcg ccatcagcgc gaccggcaat ccggtggtca
      361 gccagtgcgc cgctatttta ccggacacga gcgccgtgag cgggcgcgga gacagcgcga
      421 tctgctcgag cgtgccgtcg gcgaaatcgg acgtgaagag ccgcggcagc gacaacagcg
      481 acgctagcag cgcggccgtc cacagcacgc cggggccgag cgtgccgagc acgtcgcgtg
      541 agggggatgc ggccagcgga aaaagcgaca cgacgatcac gtagaacagc aactggacgc
      601 cgagttccgc cttcgaccgc agcgcgatgc gaacgtcgcg cgcgagtgcc cagccgaaag
      661 cggcgagcac cgattgcgtc tcgtcggcat cctgcggctc cgcgaacgtg gccggcgctg
      721 gcgcgctcac ggatcgcgct ccatcggcgc actgccactc ccctcgagcc gcagcgattg
      781 ctgccgttcg agcgcgacgg gcagcggctg atgcgaggcc gcgacgcaaa tgccgccggc
      841 ctcgagatgt gcatcgagca gattccgcag cacgtcgatt ccgtcgctgt cgagcgccgt
      901 cagcggctcg tcgaggatcc acagcggccg ccgcgaaaga tgcaggcgcg cgagcccgat
      961 gcgccgccgc tgtccctgcg acagcaggcg aaccggcagc gaccgtcggc gctcgagcgc
     1021 caccgccgcg agtgcgtcgc gaatcgaagc ggaggcggcc ggccggtcgc cgagcgagat
     1081 ccattgcgcg agattctcct cggccgtcaa ctcgtccttg agagcgggca gatggccatt
     1141 gaacgccacc gcggcgcgca atcgcgcgtc gaacggacgg gtggccgaac gccgccagcg
     1201 aatttcgccc gccgccggtg ccgtaaaccc ggccagcatg cgcagcagcg ttgtcttgcc
     1261 gctgccattg cgtcccgtca cgacgagcgc cgatccgtcg cgcacggcga acgaaacctc
     1321 ggcgaacagc gtggcgtagc cgcgttgtgc gcagagccga tcgccttcaa gcgatgcgtc
     1381 gcgctgctcc gtgagcggtg agggagacgc tcgacggccg ctcggctcgg gcacgcgcaa
     1441 ttgaaccgtc tttacggtac gacgtcgttg atgacgatca atacatcgcg ggcgccgggc
     1501 ttcacggccg cgctcgcgcc gtgcaggtcg cccggcgagg gggtggcgtt gccggttttc
     1561 gagacgcgcg cttcgacgac gacctcgttg gcgctcgaca gcttcgtcgc cggagtcatc
     1621 gccatcgaat cgtcgagccg gaacgtgcgc ggccattcgc ccgcggtggt gcgcacgatc
     1681 gcaagcggca tccgcggccc ctgcgccgag cgcgcataga tgaacagcgt gtcgctggcc
     1741 gccgtgcggc tgcgcagctt cggatcgagc gatacgctgc cggtgatcgc cgccgcggca
     1801 tcggtggcgc ccgaggctgt cgcggatgcg ccgcctttcg ccgcgccggc ctcggcgatc
     1861 atcgcatcga tctccttcac ttccgcgctg tcctgcggga actgtccctt gagctttttc
     1921 cactcggcga tcgccgcatc gtagtcgtgt cggtccatcg ctgcggacgc ggcgagcgcg
     1981 agcgccttcg gatggttcgg atcgagcgcc agcgcgcgcg cgacgagccg cgaaggctca
     2041 ccctgcagcg actgattttt catcgccagc gcgtccgccc aatcggcgag cagcggggcg
     2101 tcctcggttc cgcggatggc ggcctgcgtg aatgcggcca ccgcgtcgtc gtagcggccc
     2161 atggccgagt aggcgcgggc gagcagtcgc cagccggtcg gatcgtccgg cttttccttc
     2221 attcgcaagg cgaggcgctc gaccatcgat tcgatctgct catgcgacat cggcgcactc
     2281 gcatcggcac tgccggcgac gcgcatcgcc tccggatttc cgaaggcggc gtagagcgcc
     2341 agcgcggtga ccggcaaggc ggcggcgacg acgagcgctg cgacgtacga cgaccgcgcc
     2401 ggagacgcgg gtgcagccgc gggctcgccc gactgcagct cctgcgcgag gcgcccggaa
     2461 agctcgcgca gcgccacgtc atgttcgtcg gccgtgatcg cgccggccgc gagctcggca
     2521 tcgagctgcc gcttctggtc gcggtacacg ccggtcgttg ccgagtcctc ggacgcatcg
     2581 ctgcgcgggc cccgcgcccg cagcagcggc cacacgagcg ccgctacggt tgcagcgcag
     2641 agcgcgatgg cgacgagcca gaagagggca gcggatggca tgagtgggtt caaattatag
     2701 cgagcgctct cgtgtgttca ggctcgcgac gcccgccgcg ggcgctatca tgcgcgcatc
     2761 gcttcgaaa
//
